LOCUS       DBNGZW010000001         2395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1022680, whole genome shotgun sequence.
ACCESSION   DBNGZW010000001 DBNGZW010000000
VERSION     DBNGZW010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1022680"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="AC16JC_00005"
     CDS             complement(<1..977)
                     /locus_tag="AC16JC_00005"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5163106.1"
                     /translation="MKPRVVRPTQRGRIGLALAGGGPEGAVYEIGALRALEEAIEGLD
                     LNALYTYVGVSAGAFIASALANGITASQLARALVKHEPGEHPFAPETFFTPAYREFGR
                     RGLMLPRLLIDALLKFGRRPHDQRLLESLTRAADALPVGIFDNEPIRRYLEKIYSIKG
                     RTDDFRKLRHHLVVIATDLESGRAVRFGEPGQDHVPISRAVQASTAFPGLYPPVMIDN
                     RPCSDGVLLKTLHASVALSHGVGLLLCVNPIVPADTTAGVARGELRPGTLLRRGLPAV
                     LSQTFRTLVHSRLEIGLAAYKTRFPDADVLRFEPERDEYGMFFTRIFSFS"
     gene            complement(985..1413)
                     /locus_tag="AC16JC_00010"
     CDS             complement(985..1413)
                     /locus_tag="AC16JC_00010"
                     /inference="COORDINATES: protein motif:HMM:NF017415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HIR5163107.1"
                     /translation="MKKTKKNLEPRTIRRSAQQIWLAGLGAFALAGEEGGKLFGSLVK
                     KGEGVEKLNKSRLEKVFSRVERVGERVRDNAGRAISRIGNPFDTGMSAALHQIGVPTR
                     KEIVTLTKRVEELTRVVARSKAKAPKAVKHAQHPAQAATA"
     gene            complement(1410..1916)
                     /locus_tag="AC16JC_00015"
     CDS             complement(1410..1916)
                     /locus_tag="AC16JC_00015"
                     /inference="COORDINATES: protein motif:HMM:NF019494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HIR5163108.1"
                     /translation="MTRVITRYRGGSRKLYDTAESRYISLDEISNLIRRGDQVRVVDN
                     ATRTDVTGQVLSQVISEGERRGVSFLSAEALHEIIRSGERLLASGVRTLRREMDRLLA
                     PASEAKRRAGPPPGELDLLRTGLRQLEESLTQVGRGAGRRSPSRRPRGSPPRARQRTR
                     IRTEVEER"
     gene            2102..>2395
                     /locus_tag="AC16JC_00020"
     CDS             2102..>2395
                     /locus_tag="AC16JC_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF411 domain-containing protein"
                     /protein_id="HIR5163109.1"
                     /translation="MILSSAIRRLAAVAGGATAGLAGAALVPAPTSPLPTITVYKSPT
                     CGCCTKWIAHLRSAGFTVVAHDTDDLNGVQSAFGVPFRLGSCHTAKVGKYVIEG"
BASE COUNT          347 a          832 c          817 g          399 t
ORIGIN      
        1 gagaagctga agatgcgcgt gaagaacatg ccgtactcgt cccgttccgg ctcgaagcgc
       61 agcacgtcgg cgtcggggaa gcgcgtcttg taggcggcca acccgatctc gagccgcgag
      121 tgcaccagcg tgcggaacgt ctgcgacagc acggcaggaa gcccgcggcg caacagcgtc
      181 ccggggcgca gctcgccacg ggcgacgccc gccgtggtgt ccgccggtac gatcggattc
      241 acgcacagca gcagcccgac gccgtgactc agcgcgaccg atgcgtgcag cgttttcagg
      301 agcacgccgt cggagcacgg ccggttgtcg atcatcaccg gcggatagag acccgggaat
      361 gcggtactcg cctgcacggc gcgcgagatc ggcacgtggt cctgacccgg ctcgccgaat
      421 cggacggccc gtcccgactc gaggtccgtc gcgatgacga cgaggtggtg gcgcagcttg
      481 cggaagtcgt cggtgcgccc cttgatggag tagatcttct ccaggtagcg gcggatcggc
      541 tcgttgtcga agattcccac cggcagggca tcggccgcgc gcgtgagcga ttcgagcagc
      601 cgctgatcat gcggccgccg gccgaacttt agcagggcat cgatgaggag ccggggcaac
      661 atgaggcccc gccggccgaa ctcccggtag gccggcgtga agaacgtctc cggggcgaac
      721 ggatgctccc ccggttcgtg cttgacgagc gctcgcgcca gctggctggc cgtgatgccg
      781 ttggccaggg ccgaggcgat gaacgcgccg gcgctcacgc ccacgtaggt gtagagggcg
      841 ttcaggtcca gtccctcgat cgcctcctcc agagcgcgga gggcgccaat ctcgtagacg
      901 gcgccctccg gtcccccccc cgcgagtgcg agcccgatgc gcccgcgctg cgtaggacgg
      961 acgacccgcg gcttcacgga tcgctcaggc ggttgcggcc tgcgccgggt gctgcgcatg
     1021 cttgacggcc ttcggcgcct tggccttgga gcgggcgacc acgcgggtca gctcttctac
     1081 ccgcttggtc agggtcacaa tttccttccg cgtgggcacg ccgatctgat gcagcgcggc
     1141 gctcatcccg gtgtcgaacg gattgccgat ccggctgatg gcccggccgg cgttgtcccg
     1201 cacccgctcg cccacccgct ccacgcgcga gaacaccttc tccaggcgcg acttgttcag
     1261 tttctccact ccctcgccct ttttcaccag cgagccgaac agcttcccgc cctcttcgcc
     1321 cgccagggcg aaggccccca ggcccgcgag ccagatctgc tgcgcggacc ggcggatggt
     1381 gcggggctcc agattcttct tcgtcttctt catcgctcct ccacctcagt ccggattcgg
     1441 gtacgctgac gcgcgcgggg cggacttccc cgcgggcgcc gactggggga ccggcggcct
     1501 gcgccgcgcc ccacctgggt cagcgattct tcgagttggc gcaggccggt gcgcaggaga
     1561 tccagctccc ccggcggtgg ccccgcccgg cgcttcgctt cggatgccgg cgcgagcagg
     1621 cggtccattt cgcgccgcag ggtgcgaacc ccgctggcga gcaggcgctc gccgctgcgg
     1681 atgatctcgt gcagggcctc ggccgagaga aacgacacgc cgcgccgctc gccttccgag
     1741 atcacctgcg acaggacctg acccgtcacg tcggtccggg tcgcgttgtc caccacgcgg
     1801 acttggtcgc cgcgccggat caggttcgaa atctcgtcga gcgagatgta gcggctctcg
     1861 gccgtgtcgt agagcttgcg gctgccgccc cggtagcgtg tgatcactcg tgtcatcgcc
     1921 gcacccttgg cgtttcggtt ccgcagtgcg tcaggacgct cctgccgcac tgcgacaaat
     1981 cctaggtgtt gggggatgaa acggccttga atcggggatg agcgctttgg ggctgcttca
     2041 ccggaccgcg actacagact agtttgcggg aggccgttcc cgacttccga ggagcatgtt
     2101 catgattctg tcatccgcaa tacgacgtct ggccgctgtg gccggcgggg cgacggccgg
     2161 gttggccggc gccgcgctcg tgccggcccc gacgtccccg ctgcccacga tcaccgtgta
     2221 caagagtccc acctgcggct gctgcacgaa atggatcgcg cacctgcgct cggccgggtt
     2281 cacggtcgtc gcccacgaca ccgacgatct gaacggcgtg cagagcgcct tcggagtgcc
     2341 gttccggctc ggatcgtgcc acacggccaa ggtggggaag tacgtgatcg agggt
//
LOCUS       DBNGZW010000002         3522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_749986, whole genome shotgun sequence.
ACCESSION   DBNGZW010000002 DBNGZW010000000
VERSION     DBNGZW010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_749986"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="AC16JC_00025"
     CDS             <1..960
                     /locus_tag="AC16JC_00025"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HIR5163110.1"
                     /translation="RYFPELLEPLGAALPGRCVLDGEIVIVGTTGLDFEALLLRIHPA
                     ASRVKLLAEETPASFVAWDLLALGDEDLRGQPLAARRERLEKALAAADRPVHLSPATR
                     DRVLAEDWFRRFEGAGIDGVMAKRLDSPYRAGERSMIKVKHTRTADCVVAGFRWHKEG
                     KGKRVGSLLLGLYDDGGTLHHVGVVAAFTTAVREELVAELDPLREKALEGHPWRGWAE
                     AQEEGDAAAKGQRLPGATSRWNRGKDLSWEPLRPERVCEVAYDHMQGTRFRHAAQFVR
                     WRPDKRPEDCRYDQLEVTPAYELERVFGAGGGGRRKRGPRRRS"
     gene            complement(878..1981)
                     /gene="ligD"
                     /locus_tag="AC16JC_00030"
     CDS             complement(878..1981)
                     /gene="ligD"
                     /locus_tag="AC16JC_00030"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012935301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HIR5163111.1"
                     /translation="MGTERETVLEVAGREVTITNPDKVFFPRAGHTKLDLAKYYVSVA
                     EGALRGIAARPIVLKRYVNGADREPFFQKRAPAPHPEWIETVELRFPSGRTAREIVVR
                     DAGQLLWIVNLGCIDLNPHPVRADDLVHPDELRIDLDPTPGTSWDDVRRVTLLVRDVL
                     EERGLVGWPKTSGSRGMHVNVRIETRWSFDEVRRAALALAREVERRAPERATTRWWKE
                     ERHGVFLDYNQNAKDRTVASAWSVRPTADARVSMPLEWDEVGDCDPAAFTLATAPARM
                     AERGDAAAGIDAAAGSLDGLLGLSAEQERSGLGEAPWPPHYRKQAGEPPSVAPSRRKG
                     KGPPEAATISGAAHASVGRPRPRRRRAPARKPA"
     gene            2130..3359
                     /locus_tag="AC16JC_00035"
     CDS             2130..3359
                     /locus_tag="AC16JC_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163112.1"
                     /translation="MISLEEIAFNHDPASATADALNLRRNASDWVHVPEWRRGISVTP
                     EDSPAAYAIAQVAGHTVTIKASLSCTDPGITSAWIRAKDNVVSPPPPSGCLGWLVWLL
                     QVLAQALFGNVLGEVQARTVTFVNGQTGLVSFTLISTNLSKARVGSHTTEWRWQYRLP
                     GKKTKWKDLAITRHRIYTILDLPTAPWEQTPYQQSNVQLPWTAVLDYACQWALGAANA
                     VDAAGGVTRGVNDLGPGIVTYDCPGGGNSWYSAGGFDCTAFLERLKGGVGNGYYVNCS
                     DCATFVSTVANVLGCDLWQSRMGSGFFHLNPILGIGSSTWQPACHGIDGWADGFGYHE
                     VAWTGACGVGDNVFDACLQVDGDADPTSAPHTALLPVDMRFGNPGDLQYRDRLAVPAD
                     RGNCSPLPGTRQRRPVS"
BASE COUNT          543 a         1226 c         1211 g          542 t
ORIGIN      
        1 cgctatttcc ccgagctcct cgagccactg ggcgccgcgc tgcccgggcg ctgcgtgctg
       61 gacggcgaga tcgtcatcgt cggcacgacc gggctcgatt tcgaggcgtt gctgctccgc
      121 atccatccgg ctgcgtctcg cgtgaagctc ctggcggaag agacgccggc atccttcgtc
      181 gcctgggacc tgctggcgct gggcgacgag gaccttcggg gacagccgct cgccgcccgc
      241 cgcgagcgcc tcgagaaggc tctggcggcc gcggaccgtc ccgtacatct ctcacccgcc
      301 acgcgcgatc gcgtcctcgc cgaggactgg ttccgtcgct tcgaaggcgc aggcatcgac
      361 ggggtgatgg ccaagcgtct ggattcgcct taccgcgccg gcgagcgctc catgatcaag
      421 gtcaagcaca cgcgcacggc cgactgcgtc gtcgccggct tccgctggca caaggaggga
      481 aagggcaaga gggtcgggtc tctcctcctc ggcctctacg acgacggcgg cacgctgcac
      541 cacgtcggag tcgtcgcggc attcacgacc gcggtccgcg aagagctcgt cgccgagctc
      601 gatcctctgc gcgagaaagc gctggagggg cacccgtggc gcggctgggc cgaggcacag
      661 gaagaggggg acgcggccgc caagggacag cgcctgcccg gcgcgaccag ccggtggaat
      721 cgcggcaagg acctgagctg ggagccgctc aggccagagc gcgtgtgcga ggtcgcgtac
      781 gaccacatgc agggaactcg cttccggcac gccgcgcagt tcgttcgctg gcgtcccgac
      841 aagcggccag aagactgccg atacgatcag ctcgaggtca cgccggctta cgagctggag
      901 cgcgtcttcg gcgcgggcgg gggcggccga cggaagcgtg ggccgcgccg gagatcgtag
      961 ccgcttcggg cggtcccttt cccttccgtc gtgacggcgc gacgctaggc ggctcgccgg
     1021 cctgcttcct gtaatgcgga ggccagggcg cctcgccgag acccgatctt tcctgttcgg
     1081 cagacagccc gagcaacccg tcgagcgacc ccgcggcggc atcgattccc gccgccgcgt
     1141 ccccgcgttc agccatccgc gccggcgccg tggccagcgt gaaggcagcc ggatcgcagt
     1201 ccccgacctc gtcccattcg agcggcatgg acacacgcgc gtcggccgtc gggcgcacgg
     1261 accaggccga cgccaccgtg cggtccttgg cgttctggtt gtagtccagg aacacgccgt
     1321 ggcgctcttc cttccaccat ctcgtggtcg cgcgctcggg cgcgcgccgc tccacctcgc
     1381 gcgcgagcgc cagagccgcg cggcgcacct cgtcgaagct ccagcgcgtc tcgatccgga
     1441 cgttgacatg catgccgcgc gagcccgacg tcttgggcca gcccacgagg ccccgttcct
     1501 cgagcacgtc gcgcacgagc agcgtcacgc gccggacgtc gtcccagctc gttcccggag
     1561 tcggatccag atcgatccgc agctcgtccg ggtgcacgag gtcgtcggcg cgaacagggt
     1621 gcggattgag gtcgatgcac ccgaggttga cgatccacag aagctggccc gcgtcgcgga
     1681 cgacgatctc gcgcgcggta cggcccgacg gaaagcgcag ctccacggtc tcgatccact
     1741 cgggatgcgg tgcgggcgcc cgcttctgga agaacggctc gcgatccgcg ccgttcacgt
     1801 agcgcttgag cacgatcggc cgcgccgcga tcccgcgcag ggctccctct gcgaccgaga
     1861 cgtaatactt cgccaggtcc agcttggtgt ggcctgcccg ggggaagaag accttgtccg
     1921 ggtttgtgat cgtcacctcg cgaccggcga cctccaggac ggtctcccgc tccgtgccca
     1981 tgaagacagt ctggccgcat tcactctcgc cgcgcaacga caaaggtttg caggagcggg
     2041 acgcgcggcc ggcggtgata aggttcgcgc atggcgcggg tggcgacggc gctgtggctc
     2101 gtgctggcgg tcccgggcga ggttttccgg tgatctccct cgaggaaatc gcgttcaatc
     2161 acgatcccgc ctcggccacc gccgatgccc tcaacctgcg ccggaacgcc agcgactggg
     2221 tgcacgttcc cgagtggcgc cgcggaatca gcgtcactcc cgaggattcc ccggccgcgt
     2281 atgccatcgc ccaggtggcg ggccataccg tcacgatcaa ggcctcgttg agctgtaccg
     2341 atcccggcat cacgagcgcc tggatcaggg cgaaggacaa cgtcgtctct cctccgccgc
     2401 cgagcggatg tctgggctgg ctcgtgtggc tgcttcaagt cctggcgcag gccttgttcg
     2461 ggaacgtgct cggcgaggtg caggcgcgca ccgtcacctt cgtcaacggc cagaccggtc
     2521 tcgtttcgtt cacgctcatc agcacgaatc tctccaaagc gcgggtgggt tcgcacacga
     2581 ccgagtggcg ctggcagtac cgcctgccgg gcaagaagac gaagtggaag gacctcgcga
     2641 tcacgaggca ccggatctac accatcctcg acctgcccac cgccccgtgg gagcagacgc
     2701 cataccagca gtcgaacgtc cagctgccct ggacggccgt gctggactac gcgtgccagt
     2761 gggcgctggg cgccgcgaac gcggtcgacg ccgcgggagg cgtgacgcgg ggcgtaaacg
     2821 accttggacc cggaatcgtg acgtacgact gtcccggcgg tggcaactcc tggtacagcg
     2881 ccggagggtt cgactgcacc gcgtttctcg aacggctcaa gggaggcgtc ggcaacggtt
     2941 actacgtgaa ctgcagcgac tgcgcgacct tcgtctccac cgtcgccaac gtcctggggt
     3001 gcgacctgtg gcagtcccgc atgggctccg gcttcttcca cctcaatccc atcctgggga
     3061 tcgggagcag cacgtggcag ccggcctgcc acggcatcga cggctgggcg gacggcttcg
     3121 gctaccacga ggtggcttgg accggggcgt gtggcgtggg cgacaacgtg ttcgacgcct
     3181 gcctgcaggt cgacggagac gccgacccga cgtcggcccc gcacactgcc ctgctgccgg
     3241 tcgacatgag gttcggcaac cccggcgatc tgcagtatcg cgaccggctg gcggttccgg
     3301 cggaccgcgg caactgcagt ccgctcccgg gaacgcgaca gcgccgaccg gtgagctgag
     3361 agagggtgcc atggcgctac cacccgaact gaaggaatct cttcgcaggc gacacaagtt
     3421 cgtcgactgg cagggggctg gagagacaac cggcggtccg ccgcttcgcg gtctcgagtt
     3481 caagggagac gagctgcccg gatgggaacc cgtgaaggcg cg
//
LOCUS       DBNGZW010000003         4169 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_477285, whole genome shotgun sequence.
ACCESSION   DBNGZW010000003 DBNGZW010000000
VERSION     DBNGZW010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4169
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_477285"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..352
                     /locus_tag="AC16JC_00040"
     CDS             <1..352
                     /locus_tag="AC16JC_00040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5163113.1"
                     /translation="VSTFTLCTIPGVVDAVRGLRRVLRPDGRLIFFELGISPDPAVQR
                     WQKRLEPVHRWLFGGLRLTRDIPSLLQDGGFQIEQIEAGHLARFPKASSYCWWGTAIH
                     NAGSGWRHDVPSNN"
     gene            730..2187
                     /locus_tag="AC16JC_00045"
     CDS             730..2187
                     /locus_tag="AC16JC_00045"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020636552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR5163114.1"
                     /translation="MPRPPHLTRREFVRTGTAIAAAAVLDGPVIHVARRAAGAGPSFQ
                     RALPIPPVLAPVRRDATTDYYEIVQRAGRGLLLPGMPTTVWGYDGLWPGPTIRARRGR
                     TVVVRHTNALVVPTVVHLHGGETPPDSDGFPTDAVMPGATRTYTYPNSQPAATLWYHD
                     HAMDHTGRSIYMGLAGFYLLEDAAEAALGLPRGACDVPLLIQRRACDRGGELHYNPLR
                     QLGASGPIVTVNGAPTPHLEVAARKYRFRLLNGSNATPLRLSLSSGDPLVVIATDTGL
                     LPAPLSCSSVRLGMAERVEVVVDFSRLPPGSRLFLVDEAVEGDQRWVMRFDVIRAEHD
                     ESRLPERLGEPPDLRALDASRTRDFVFAGGPRGFPPQAHWTINGRDFDTGRPIATPRY
                     GDVEIWRFANHRRFGVLGMLHTVHIHLVRFLILDRNGRAPEPYERGWKDTVALEKGDV
                     VRVVMRFGGHKGRYLVHCHNLEHEDASMMARYDVV"
     gene            2195..2707
                     /locus_tag="AC16JC_00050"
     CDS             2195..2707
                     /locus_tag="AC16JC_00050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5163115.1"
                     /translation="MLERFVRGDRDAFEALYREYGAQVYAWVLRMVRDRGAAEDITME
                     AFWRIYKARARFDPGREFWPWARRIATNLALDNMRSRPPEVGLSDELAGTDATDPGVR
                     ADVVGHIRAALARLPVHLRAVALLALIEDRPYGEIGEALGLTPNAVKLRVFRAVRLLR
                     QDLASRGITP"
     gene            2704..2916
                     /locus_tag="AC16JC_00055"
     CDS             2704..2916
                     /locus_tag="AC16JC_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163116.1"
                     /translation="MTDPELTRLLKQAIPPLGVEPPRRDLWTEVRAGLDRGERRVNWL
                     DWVIATAAAAGFAALPELLPALLYLL"
     gene            2924..3337
                     /locus_tag="AC16JC_00060"
     CDS             2924..3337
                     /locus_tag="AC16JC_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163117.1"
                     /translation="MNAHRYLRAYLAGIAVPTMVLLVVLSGLTVARHLGRVPGPVERV
                     IVFPMAVVPNLWGAWNMLYAWLVLRRRIPIGVFGALLPMIVAPTAVVLGTAMGVELPR
                     FAVQALPVVVGVVTGAYYLLWKYAVRFLNEILEIA"
     gene            complement(3698..>4169)
                     /locus_tag="AC16JC_00065"
     CDS             complement(3698..>4169)
                     /locus_tag="AC16JC_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5163118.1"
                     /translation="ADLDPRLRPLSKQNFDLAGIDIVVNTHLHFDHCGGNHLFAGRPI
                     YVQRRELDDARNEDDYTIREWVEAPGVRYVPVDGALELLPGLRLVPAPGHTRGMQVVV
                     VETGGRPVVVGGDVAVWFGELDEPHTEGQLRVRALDPELVWLAHEHEPWRPRTV"
BASE COUNT          610 a         1390 c         1401 g          768 t
ORIGIN      
        1 cgtgagcacc ttcacgttgt gcacgattcc tggcgtagtc gatgccgttc gaggccttcg
       61 ccgcgtctta cgaccagacg gccggctcat cttctttgag ctcgggattt cgcctgaccc
      121 ggccgtacaa cggtggcaga aacgattgga gccagttcat cgctggttgt tcggaggttt
      181 gcgtttgaca cgcgacatcc cgtcgctgct ccaggatggc ggctttcaga ttgagcagat
      241 cgaggcgggg catctagccc gatttcccaa ggcgtcctcc tactgctggt ggggtaccgc
      301 gattcacaat gccggctctg gatggcgcca tgatgtgccg tctaacaact agaattcatg
      361 gaccgtgtta tggaccgtgt tagcgcctgt cttcgtttga ttttcgggag tcttctggct
      421 gatgttgttt gacgtaagtg cttgatttcc ctgcaggatg tcaaaaagcg accccctgtg
      481 gcccttgatg gggttcaggg gtcgcgggtt aaatcccgca ctaaagtact tccttcggtt
      541 gccgtcccga ctgaatgaag atcaagcctc gcatcgactt ccggtgtggg gctttttcct
      601 cgtctgccca tgcggtgagt tcggccgcca atgcggcctc cgggccaccc caggagcctc
      661 gaatgccggg accgggagcg acgttcggaa acgcgccccc gtcccgggca gtatctgggg
      721 gagagaccga tgcccagacc tccccacctc actcgccgcg agttcgttcg caccggtacg
      781 gctattgccg ccgccgcggt cctcgacggg cccgtgatac atgtcgcgcg ccgagccgcc
      841 ggcgcagggc cttcgttcca gcgcgcccta ccgattccgc cggtgctcgc gccggtgcgg
      901 cgcgacgcca cgaccgacta ctacgaaatc gtccagcgtg cggggcgcgg cctgctgctg
      961 cccggcatgc cgactaccgt gtggggttac gacgggcttt ggcccggacc cacgatccgg
     1021 gcgcgacgcg gccgcacggt cgtcgtccgg cacacgaacg cgcttgtggt gccgacggtc
     1081 gtgcacttgc acgggggcga aacgccgcct gattcagacg ggtttcccac cgatgcagtg
     1141 atgcccggtg cgacccgcac ctacacctat cccaacagcc agcccgccgc gacgctgtgg
     1201 tatcacgacc acgcgatgga ccacaccggc cgcagtatct atatgggcct ggcgggattc
     1261 tacctgctgg aggatgcggc tgaagcagca ctggggctgc cgcgcggcgc gtgcgacgtg
     1321 cccctcttga tccagcgccg cgcctgcgat cggggcggcg aactgcacta caatccgctc
     1381 cgccagctcg gcgcgagtgg cccgatcgtg accgtcaacg gcgcgcccac gccgcatctc
     1441 gaagtcgcgg cgcggaaata ccgctttcgc ctcctcaacg gctcgaacgc cacgcccttg
     1501 cggctcagcc tctcgagcgg ggacccgctg gtcgtcatcg ccaccgacac cgggttgctg
     1561 cccgcgccgc tgtcgtgctc gagcgttcgc ctcggcatgg ccgagcgcgt cgaagtcgtg
     1621 gtggacttct cccgcctgcc accgggcagc cggctctttc tggtggatga ggcggtcgaa
     1681 ggcgaccagc gctgggtcat gcggttcgac gtgattcgcg ccgaacacga cgagagccgt
     1741 ctacccgaac gtcttggcga accgcccgac ctgcgggcgc tggacgcctc gcggacccgc
     1801 gacttcgttt tcgcgggcgg gcctcggggc tttccgccgc aggcgcactg gacgatcaac
     1861 gggcgtgact tcgataccgg ccgccccatc gcgacgcctc gctacggcga cgtcgagatc
     1921 tggcgtttcg ccaaccaccg gcgtttcgga gtcctcggca tgctgcacac cgtgcacatc
     1981 cacctcgtgc ggttcctgat tctcgatcgc aacggccgtg cccccgagcc ctacgagcgt
     2041 gggtggaagg acaccgtcgc gctcgagaag ggtgacgtcg tgcgcgtggt gatgcgattc
     2101 ggcggacata agggccgcta tcttgtgcac tgccacaacc tcgagcacga ggatgcgagc
     2161 atgatggccc gatacgacgt ggtgtgatct ggggctgctc gagcggttcg tccggggcga
     2221 ccgcgacgcc ttcgaggcgc tgtatcgcga gtacggcgcg caggtgtacg cgtgggtcct
     2281 gcggatggtc cgcgatcgcg gcgcggcgga ggatatcacg atggaagcgt tctggcggat
     2341 ttataaggcg cgtgcgcggt tcgatccagg gcgcgagttc tggccgtggg cgcggcgcat
     2401 cgccacgaac ctcgcgctcg acaacatgcg ctcgcgaccg ccagaggtcg gcctcagcga
     2461 cgagctggcg ggcaccgacg ccactgatcc cggcgtgcgc gccgacgtgg tgggacacat
     2521 ccgggctgcc ctcgcgcggc tgccagtgca tttgcgcgcc gtcgcgctcc tcgcgctcat
     2581 cgaggatcgc ccctacgggg agatcggaga ggcgctcggc ctcacgccga acgcggtgaa
     2641 actgcgcgtg tttcgtgccg tgcgactgtt gcgccaggac cttgcgagca ggggaatcac
     2701 gccgtgaccg accccgagct gacccgcctg ctgaagcagg cgatcccccc gctcggcgtc
     2761 gagccgccgc gacgcgacct gtggaccgag gtgcgggccg gactggaccg cggcgagcgg
     2821 cgggtcaact ggctcgactg ggtaatcgcg acagcggccg cggcaggatt cgcggcgctg
     2881 cccgagttgt tacctgcgct gctgtacctg ctgtgaggtg aacatgaacg cgcatcgcta
     2941 tctccgcgcc tatctcgcgg gtatcgccgt cccgacaatg gttctcctcg tggtcctgag
     3001 cgggttgacg gtcgcccgcc acctgggacg tgtcccgggg ccggtggagc gcgtgattgt
     3061 ctttccgatg gcagtggtgc cgaatctatg gggtgcgtgg aacatgctct acgcatggct
     3121 ggtactgcga cgtcgcatcc ccatcggcgt attcggggcc ctgctcccga tgatcgtcgc
     3181 cccgacagcg gtggtgctgg gcaccgccat gggcgtcgag ttgccccgct tcgccgttca
     3241 ggcccttccg gtagtcgtgg gagtggtgac gggcgcctac tacctgctct ggaagtacgc
     3301 cgttcgcttc ctcaacgaaa tcctcgaaat cgcttgaggg gcgctcgatg ttcgggtcca
     3361 ccacacccac cgcgtccccg cttcactcaa ccacatacgg ctcaggttca agcggctgtg
     3421 ggacggcgac tactccggcg tcccatccga ctcgtccggc cggatctacg tcgtctggtc
     3481 ggatactcgc cccggagttt tccaaccgcg tctggcgcgg cttgatgtgc gcgctgcgcc
     3541 ccgtgctgtg cgttttgttg ttagtcctgc cggcttcagc ggctcgagac ggacactgga
     3601 gcgggacgat cgttccgtcg tcccaaagca cggcgagcgg ctggtatcgc ggtccagacc
     3661 ggaacccggg aggtccagag agcgccggcg tgatgcctta gacggtgcgg ggtcgccacg
     3721 gctcgtgctc gtgcgcgagc cagaccagct cggggtcgag cgcgcgcact cgcagctggc
     3781 cttcggtgtg cggctcgtcg agctcgccaa accacaccgc cacgtcgccg cccacgacga
     3841 ccgggcgccc gccagtctcg acgacgacga cctgcatgcc gcgggtgtgg cccggcgccg
     3901 ggacgagccg gagcccgggc agcagctcga gtgcgccgtc gaccggcacg taccgcacgc
     3961 cgggcgcctc gacccactcg cgaatggtgt agtcgtcctc gttgcgcgcg tcgtcgagtt
     4021 cccgacgctg gacgtagatc ggcctgccgg cgaagaggtg gttgccgccg cagtggtcga
     4081 agtgcaggtg tgtgttgacg acgatgtcga tgccggcgag gtcgaagttc tgcttgctca
     4141 gtggacggag gcggggatcg aggtcggcc
//
LOCUS       DBNGZW010000004        16875 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1840827, whole genome shotgun sequence.
ACCESSION   DBNGZW010000004 DBNGZW010000000
VERSION     DBNGZW010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 16875)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16875)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16875
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1840827"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="AC16JC_00070"
     CDS             <1..404
                     /locus_tag="AC16JC_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163119.1"
                     /translation="VSGAGFLLGSPVCVALILTNNLRLFAPLVFTTYFFYTWYNGPLA
                     AVIFDVVPAAIRASVMGAFLLFSHLAGDAIAPPLIGYLSDRLQAVWHLSADEALRRAM
                     LLLPTVGLAGGLVLLLALRTVGRDMKRERRS"
     gene            401..1399
                     /locus_tag="AC16JC_00075"
     CDS             401..1399
                     /locus_tag="AC16JC_00075"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR5163120.1"
                     /translation="MKIAVVFDTPHPGWEDADYKREVEAGTVEEAEYDVARALMQNDH
                     EVLMVGIVDDVRPLVDGLRAFEPALVFNCCEGFRGSAQYEYAVAAVLEMHGWRHTGST
                     PTGLLVARNKSMSKKILKHHGIRVPEFATFHAGDAFLRPSTLRFPLIVKPLLEDASVG
                     ISQASVVSDDESLAERVTFIHDKFRQAAIAEELVEGRELYVSLIGNDDPEVLPVVELH
                     FGKVPKAERRIVTYMAKWNDEYRERHHIRSGFARLSEAVRGKIRAVCETAFHALWLQD
                     YARMDLRLTHDHEVYVLEANPNPFIAYGHEFANAAEKAGMNYRAFIQRIVDEALRR"
     gene            1396..2421
                     /locus_tag="AC16JC_00080"
     CDS             1396..2421
                     /locus_tag="AC16JC_00080"
                     /inference="COORDINATES: protein motif:HMM:NF027219.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR5163121.1"
                     /translation="MSGSVWSAVRTLVRRREVPRPELRCPRCRAELEPFWYHCPNCGK
                     AQRWGDTQLQTGVECRYCRWMVSDSFSFCPWCGRNISDEQSSLEPLKKPKGFKYERRC
                     KRCHGGLQYPMQWCPWCGKVQRWKYERFQNDCPHCDRGVDDWMETCPWCGKDATGRDL
                     VRQALRRVRSLLIVSRIKDWDYRILLRPGVSGVSPAAPKVIEIDRRYVTGKRRRDEIS
                     WNMLTGLILHELGHSFLYHNWSWTRSGRFRRTFGEVRKAYRVADEHWVDFERRRVATT
                     LVDHVSRYAGTHPQEDFAETFRFYVTRRGRLRELFGEFGRKRKGVIVFEKFLVLHDYV
                     RSLRGWR"
     gene            2421..2699
                     /locus_tag="AC16JC_00085"
     CDS             2421..2699
                     /locus_tag="AC16JC_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002622594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HIR5163122.1"
                     /translation="MDPTDRSARLRAVAAARWKVAIALTGAMIVLYFGFVMLIAFDKP
                     LLGRLLVPGLSLGIVLGAGVIVCSWLLTWIYVRWANTRYDAELRALDE"
     gene            2692..4254
                     /locus_tag="AC16JC_00090"
     CDS             2692..4254
                     /locus_tag="AC16JC_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015126991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR5163123.1"
                     /translation="MSSLTAQAAPATSIGEPNGVAMTFFFLFIAATLGITYWAARRTR
                     TTEHFYAAGRTISPAQNGLALAGDYMSAASFLGIAGLVATTGFDGLIYSTGWLVGWPV
                     VLFLVAEPLRNLGKYTFADVVAARLSQTPVRLAAALGTLSTVALYLIAQMVGAGSLIR
                     LMFGLHYETAIVLVGAAMIVYVLFGGMLATTWVQIVKAGLLLSGAAILTVLVLSRFGM
                     NPAALFAAAAERYGDAVLAPGRLVAKPLDAIALGMALMFGTAGLPHILMRFYTVPDAK
                     AARKSVFYATGLIGAFYLMTFVLGFGAMVLVGPDAIRQVEPGGNMAAPLLAEFLGGRA
                     FLGFIAAVAFATILAVVAGLTLSGAAALSHDLWVSVVRRGRADTAEQLTVARVATVGL
                     GILAVLLGITFKGQNVAFMVGLAFAIAASANFPALVLSMFWRGYTTAGAVTSMVVGTV
                     STLVLIWLSPTVQVDVLKHPTAWFPLKNPALVTIPLSFAVGILASFIAARTHEAEAYG
                     RMERQKHLGGAA"
     gene            complement(4251..4715)
                     /locus_tag="AC16JC_00095"
     CDS             complement(4251..4715)
                     /locus_tag="AC16JC_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163124.1"
                     /translation="MKPVVAVALAVSVLLTTTTATAHAQRSHIGPHVGYNFDVDRALI
                     GAQLLLPLGRSVELYPSFDYYFVDAGSLVGFSGDLKFRVPTGGPSVLYVGGGINFLRA
                     SAGGVSDTDTGWDLPFGLESRLGMTHPYVEGRVLNHSGSSFQLVAGLNLTLF"
     gene            4962..5692
                     /locus_tag="AC16JC_00100"
                     /pseudo
     CDS             4962..5692
                     /locus_tag="AC16JC_00100"
                     /inference="COORDINATES: protein motif:HMM:NF016241.7"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
     gene            5704..6252
                     /locus_tag="AC16JC_00105"
     CDS             5704..6252
                     /locus_tag="AC16JC_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163125.1"
                     /translation="MRRLLRALPLVAAVPLAGQRPARPHVEVPTIAPRPEDVATLDGI
                     LQAFYDVISGPAGQPRQWGRDRTLYIPNVRFVSLSTDSTGAAHASVMDHQTYVDRTND
                     AFVRRGFFETEIHRTTHRFGNVVHVFSTYEMRERLDGPVIGRGINSVELFWDGTRWWI
                     AVAQWDDERPDNPIPPEYLPGS"
     gene            6249..7040
                     /locus_tag="AC16JC_00110"
     CDS             6249..7040
                     /locus_tag="AC16JC_00110"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5163126.1"
                     /translation="MILSLGERRRYYRAALRFARLARRVGAKRIAAGFRTRLKRDASF
                     VTEVDLAVERAVRAAIGRAFPRHGVFGEELPPSHPDAEFQWILDPIDGTTSLKNGIPF
                     YGTIIALHHRGRPLVGVIDLPALDRCYSAAAGLGAWRDGRRLRLRDGAPRDLPREVIS
                     VGDRTRFVQCRAGARFDRLMREHGQVRGYIDCIAHAFAAEGVIGAAVDYGVKLWDIAA
                     TQLLVEEAGGRYVCAYRSRTKDPVLYGIVCGKPAVVRWLMRRYFG"
     gene            complement(7037..8782)
                     /locus_tag="AC16JC_00115"
     CDS             complement(7037..8782)
                     /locus_tag="AC16JC_00115"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HIR5163127.1"
                     /translation="MGGRIAQPRGRAPRRSGGELAGEAPFSHRTRAAALESIQSQPVD
                     LLVIGGGITGAGIARDAALRGFRTALVDMGDFGAGTSSRSSRLIHGGLRYLEQYEFGL
                     VLEASRERRVLLHIAPHLVRPLRFLFPLYRGGRVPPWKLRAGMWLYDALASFRNTHRH
                     RWLSPRKALEAEPGLRDRDLQGAAVYWDAQTDDARLVVAALRGAAQSGALCANYTAIT
                     SFVRAEGKIIGAVLRDAISGEERIVRALSIVNATGPWVDRVRRLDDPNVKPLMRLSKG
                     AHAIVRRERLGLRDAITLASPIDGRVMFLLPWNDLAYIGTTETEEDVAPEAARATGDD
                     VTYLLRSANAVFPRARLAPADVIATWAGLRPLLAPERDIPAGRVSREHRIVESPSGLV
                     TIAGGKLTTFQVMGRDVVDVVARRLRQVDGRPLPRPPATEAMPLPGGEIAALDVMMQS
                     ARMKELPATTARHLVDSYGTESAAILNLVAKNRVLGGPLVTGRPEIRAEVVYAVEREM
                     AMRLSDVLIRRLHLFYEDPQHGAGVAATIAKKMAELLGWDSTREDDEIGEYLEEVRKS
                     REFLREVGRTSQVPR"
     gene            8977..10236
                     /locus_tag="AC16JC_00120"
     CDS             8977..10236
                     /locus_tag="AC16JC_00120"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR5163128.1"
                     /translation="MAFPNGRRVAIVAGCRTPFCKAGTVLKDARAVDLARHAARELIE
                     RTNLDPAKVDEVIFGQVVASPLVPNIAREVSLLPQFPKEVPSFSVNRACASSNQAITA
                     AHDQIAQGHADVILAGGAEALSAIPILASRRLSDILVSASKARTLGARLATFARIRPR
                     DLIPVAPAIAEPSTGQSMGQSAEKMAKENHIPRDAQDRWTLRSHQLAAKGTADGRLTA
                     EIAPWLPLRAGGEVVQADNGIRSDTTLEQMAKLKPVFDRRYGSVTAANSSPLTDGASA
                     VLLMAEDVARALGYEALAYIRSYAVAAVDPGWQLLQAPVWAVPKALERAGIQWKDLGL
                     IEIHEAFASQVLSNLQGFAEKGWEINEEIINVMGGSIAIGHPFGATGGRLFTTLSNEM
                     KRRDVQFGLISVCAQGGMASAVVLERR"
     gene            10236..12359
                     /gene="fadJ"
                     /locus_tag="AC16JC_00125"
     CDS             10236..12359
                     /gene="fadJ"
                     /locus_tag="AC16JC_00125"
                     /EC_number="1.1.1.35"
                     /EC_number="4.2.1.17"
                     /EC_number="5.1.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HIR5163129.1"
                     /translation="MAAITWEVKDGVAVVTLDCPGAPVNVISQVVKDEVYAMLLALER
                     DPQVRAVAFFSGKTDNFIAGADIEEFVRLKSAAEAEQLSRDGQAMLDRVAQFSKPIAV
                     GVHGACLGGGLEFAMACHYRVATDHPKTQLGLPEVQLGILPGASGCQRLPRLIGARAA
                     LDIILAGRSERAAKALRLGMVDELVPPSILKDVTIAAARRMADRGVMLRRRRGGFMGL
                     LLDGNPLGRLLVFRLARKQVLSQTKGNYPAPLAALEAVRHGLTHGMPAGLQREARLFG
                     ELAVGDVSRKLVQIFFATRDLKKDFGVAAAPPPAEVRRLGIIGSGFMGSGIAGTAVVQ
                     AGVDVRLKDSDLPRVAKGILAARAIVDERLTRRRISRYEHARLVALLSGGDSYAGFGG
                     AGLVIEAVFEELEVKRQVLRECEAVLPAGAVFATNTSTIPIGRIAEVARRPEQVIGMH
                     FFSPVARMPLLEVIPSGSTAPQTVSTAVGFGRRLGKTVIVVRDAPGFWVNRILGPYMN
                     EAAHLLLSGVGAEDIDRAMVRFGFPVGPITLLDEVGLDVAQHAGEVLEGAFGERLAPP
                     KALAALVKDGRLGRKAGKGFFTYRGGKKGPVDSGVYPLLGVQPNGGIPTADIVQRLTY
                     AMLNEAARAAAEGVVRTPRDGDIGAIYGFGFPPFRGGPLRYFDDQGADHVYADLSRLA
                     ERLGSRFEPCPAIVDLARARKRFYP"
     gene            12359..13291
                     /locus_tag="AC16JC_00130"
     CDS             12359..13291
                     /locus_tag="AC16JC_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR5163130.1"
                     /translation="MLRAGLLWLSEQPRISRFVRRNRLAGRLAGRFVAGETVDQALAA
                     LRDLNAAHISATVDLLGESVRRPEEARAARDTYLQLLDRIHAASADANVSVKLTQMGL
                     DIDEALCVETLRAILQRAREHGSFVRIDMEGSPYTQKTLDLFTRTVFPEFGQTAGVVL
                     QSYLRRTAADVEAMILLGARVRLCKGAYKEPATIAFPDKRDVDANYIACLERLLDRGS
                     YPAIATHDERIITHARAYARGRGVDPSRFEFQMLHGVRRDLQQELRRAGYRVRVYVPF
                     GSHWYPYLMRRLAERPANIGFMLGNIFRESLRRR"
     gene            13327..13611
                     /locus_tag="AC16JC_00135"
     CDS             13327..13611
                     /locus_tag="AC16JC_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163131.1"
                     /translation="MHQRPPAFSGSDGAAYSVDALVDDDPDPAGRFGAALLFIRFSEG
                     GERAIGHLETDYLAFGSTPEAARDPVLALTLQEVKAHLDRCIARKGVAGG"
     gene            13608..14552
                     /gene="rsgA"
                     /locus_tag="AC16JC_00140"
     CDS             13608..14552
                     /gene="rsgA"
                     /locus_tag="AC16JC_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HIR5163132.1"
                     /translation="MTTGVVLARAGSRFRVHTADGERIAVLRGRLKLPDDDRLVAGDV
                     VELALHGDGPATVERVFPRRSVLARRRATGGHGARRAQPIAANVDQVVVVAAARDPEP
                     SARMLDRFLVIGEANGLPAILVVDKVELDRSVIGLLQQRYAHAGYQVLSTSVKVPEGL
                     PALRDLLRGRESVFTGASGVGKSSLLNALEPGLKLRIGAISEKWRTGTHTTRAAELVP
                     LALGGYVVDTPGMREVGTWGIAPDELGACFPEFRPFLDGCRFDNCRHLAEPGCAVREA
                     ARTGQVDPDRLVSYERIYEEISVSSWSSARRRGSRPSA"
     gene            complement(14497..14850)
                     /locus_tag="AC16JC_00145"
     CDS             complement(14497..14850)
                     /locus_tag="AC16JC_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006285470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HIR5163133.1"
                     /translation="MSALILATETPVAFREAQSAVDRWVSQYKEGYFSPLTNLARLSE
                     EVGELAREINHRFGEKPKKAEEAEGDLAMELGDILFVVICLANSQGIDLDEAFAAVMR
                     KVATRDAERWTRRKR"
     gene            14837..16096
                     /locus_tag="AC16JC_00150"
     CDS             14837..16096
                     /locus_tag="AC16JC_00150"
                     /inference="COORDINATES: protein motif:HMM:NF018004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HIR5163134.1"
                     /translation="MSADTPLVTPFIGERYADGAALSRLIAPPYDIISQEARRRYAAT
                     DPHNIVHLILPEAAGDVNRYRAAAEHLAAWRAEGVFRADSAPSVYVVAQAFSTPAGER
                     LTRVGMFAAVSAEPYETRRIRPHEKTHRGPKVDRLDLLRATHANLESIFLLAPDHGGT
                     LADGLRAVAEGPRPPDASAELDGVQIRLWVQSGAEADRLSAAAAAPLYIADGHHRYET
                     AVAYAGENPAARRLLAFVVSARDPGLVVLPTHRVVYAADRDVSALAGAWQRFFEVRPV
                     PPPADRVRLLEEAGRGRVACIVATEGGQHDLLLTLRDDAPLDDIVELGRTPAVRGLDV
                     AIVERLVVGEILGAGKSTPTLTYTPDPTPALQVAHLGKALAAVLLNATKVEQVFAVAD
                     AGDVMPPKSTYFIPKVPSGLVLRRVDA"
     gene            16114..16380
                     /locus_tag="AC16JC_00155"
     CDS             16114..16380
                     /locus_tag="AC16JC_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163135.1"
                     /translation="MPGRPRERILANLETVYREAYDRAKAAGDERRMLDLDAAYQREQ
                     LLLEVLLDIRDALQAAPVPREAAPSQGVDPLAALDTIRRVTKLR"
     gene            16399..>16875
                     /locus_tag="AC16JC_00160"
     CDS             16399..>16875
                     /locus_tag="AC16JC_00160"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5163136.1"
                     /translation="MRHALPAAGLSVLLVAACGGPKADTVVVNGMVWTGLSSGEAQPG
                     AVAIRAGKILAVGDSADVARTVGSHTSVIDAHGGLVLPGFADGHTHFVQGGFQLASLD
                     LRDAATPEEFVRRIAAFAKTRQPGQWIQGGDWDHTLWRGQPLPRREWIDSVTPDNPV"
BASE COUNT         2420 a         5872 c         5898 g         2685 t
ORIGIN      
        1 cggtctccgg cgccggcttt ctgctgggct cacccgtctg cgtggcgctg atcctgacga
       61 acaatctccg gctgttcgcg ccgctcgtgt tcacgaccta tttcttctat acgtggtaca
      121 acggccccct cgctgcggtg atcttcgacg tcgtgccggc tgccatccgg gcgtcggtga
      181 tgggggcgtt cctgctgttc tcgcacctgg ccggcgacgc gatcgcgcca ccgctgatcg
      241 ggtacctgtc cgaccgcctc caggcggtgt ggcacctgtc ggccgatgaa gccctgcggc
      301 gggcgatgct gctgttgccg acggtgggcc tggcgggcgg gctcgtgctg ctcctcgcgc
      361 tgcgcaccgt ggggcgggac atgaagcggg agcgccggtc gtgaagatcg cggtcgtgtt
      421 cgacacgccc cacccggggt gggaggacgc cgactacaag cgcgaggtcg aagccgggac
      481 ggtcgaggaa gcggagtacg acgtggcgcg cgcgctgatg cagaacgacc acgaggtgct
      541 gatggtgggg atcgtggacg atgtgcggcc gctggtggac ggactgcgcg ccttcgagcc
      601 cgcgctcgtc ttcaactgct gcgaggggtt tcgcggcagc gcccagtacg aatacgccgt
      661 ggccgccgtg ctggagatgc acgggtggcg gcacaccggg tccacgccca cgggactgct
      721 cgtggcgcgc aacaagtcga tgagcaagaa gatcctcaag caccacggca tccgggtgcc
      781 ggaattcgcc acgttccacg cgggggatgc gttcctgcga ccctccacgc tgcgtttccc
      841 gctgatcgtg aagccgctgc tggaggacgc gtcggtgggg atctcgcagg cctccgtggt
      901 gagtgacgac gaaagcctcg ccgaacgggt gaccttcatc cacgacaagt tccggcaagc
      961 ggccatcgcc gaggagctgg tcgaagggcg cgagctgtac gtctcgctga tcggcaacga
     1021 cgacccggag gtcctgccgg tggtggagct gcacttcggc aaggtcccca aggcggagcg
     1081 gcgcatcgtc acctacatgg ccaagtggaa cgacgagtac cgcgagcgcc accatatccg
     1141 ctcggggttc gcccgtctct ccgaggcggt gcgcggcaag atccgcgcgg tgtgcgagac
     1201 ggcgtttcac gcgctgtggc tgcaggacta cgcccgtatg gacctgcggc tgacgcacga
     1261 ccacgaggtg tacgtgctgg aggcgaaccc caaccccttc atcgcgtacg gtcacgagtt
     1321 cgccaatgcg gcggagaagg cggggatgaa ttaccgcgcg ttcattcagc ggatcgtgga
     1381 cgaggcgcta cggcgatgag cggctccgtc tggtctgcgg tacggaccct ggtgcggcgc
     1441 cgggaggtgc cacggcccga gctgcgctgc ccgcgctgcc gcgccgagct cgagcctttc
     1501 tggtaccact gtcccaactg cggcaaggcg cagcggtggg gcgatacgca gctccagacc
     1561 ggggtcgagt gccggtactg ccgctggatg gtctcggaca gcttctcgtt ctgcccctgg
     1621 tgcggcagga acatcagcga cgagcaatcc agtctggagc cgctcaagaa gcccaaggga
     1681 ttcaagtacg agcgccgctg caagcgctgc cacggcggcc tccagtaccc gatgcagtgg
     1741 tgcccctggt gcggcaaggt gcagcgctgg aaatacgagc ggttccagaa cgactgtccg
     1801 cactgcgacc gcggcgtgga cgactggatg gagacgtgcc cgtggtgcgg caaggacgcg
     1861 acggggcgcg atctcgtgcg ccaggcgctg cgccgcgtgc gcagcctgct catcgtgtcg
     1921 cgcatcaagg actgggatta ccgtatcctg ctgcgcccgg gggtgtccgg cgtctccccg
     1981 gcggcaccca aggtgatcga aatcgaccgc cgttacgtga cggggaagcg ccggcgtgac
     2041 gagatctcgt ggaacatgct caccggcctg atcctgcacg agctggggca ctcgtttctc
     2101 taccacaact ggagctggac gcgctcgggg cggttccgcc gtaccttcgg cgaggtgcgc
     2161 aaggcctatc gcgtggcgga cgagcactgg gtggacttcg agcgccgccg tgtagcgact
     2221 acgctcgtcg atcacgtgag ccgttacgcg gggacgcacc cgcaggagga ttttgccgag
     2281 acgttccgct tctacgtgac gcggcgcggc cggctgcggg agctgttcgg cgagttcggt
     2341 cgcaagcgca aaggggtaat cgtcttcgag aagttcctgg tgctgcacga ttacgtgcgg
     2401 agcctgcgag gctggcggtg atcgacccga ccgatcggag tgcccggctt cgggccgtcg
     2461 cggcggcacg ctggaaggtc gcgatcgccc tcaccggcgc gatgatcgtg ctgtacttcg
     2521 gcttcgtcat gctgatcgcc ttcgacaagc cgttgctggg ccgcctgctc gtcccgggtc
     2581 tctcgctggg gatcgtgctc ggggcgggtg tcatcgtgtg ttcctggctg ctcacctgga
     2641 tctacgtgcg ctgggcgaac acgcgctacg acgccgagct gcgcgcgctc gatgagtagc
     2701 ctcacggcgc aggcggcccc cgccacgagc atcggcgagc cgaacggcgt cgccatgacg
     2761 ttcttcttcc tgttcatcgc ggccacgctc gggatcacct actgggccgc ccgccgcacg
     2821 cggacgacgg agcatttcta cgccgccggg cggaccatca gtcccgcaca gaacggcctc
     2881 gcgctcgccg gcgactacat gagcgcggcg tcgttcctgg gcatcgcggg cctcgtggcg
     2941 accaccggat tcgacggcct gatctactcg accgggtggc tcgtgggatg gccggtcgtg
     3001 ctgtttctgg ttgccgagcc gctgcgcaac ctcggcaagt acaccttcgc cgacgtcgtc
     3061 gccgcccggt tgtcgcagac cccggtccgc ctcgccgcgg cgctcggcac gttgtcgacc
     3121 gtcgcgctgt acctcatcgc ccagatggtc ggcgcgggca gtctcatccg gctgatgttc
     3181 ggcctccact acgagacggc catcgtgctg gtcggggcgg cgatgatcgt gtacgtgctg
     3241 ttcggcggca tgctcgcgac gacatgggtg cagatcgtca aggcggggct gctcctctcg
     3301 ggcgccgcga tactcaccgt gctcgtgctg tcgcgcttcg ggatgaatcc cgcggcgctg
     3361 ttcgccgcgg ccgccgagcg ctacggcgat gcggtgctgg cccccggccg gctcgtggcg
     3421 aagccgctcg acgcgatcgc gctcggcatg gcgctcatgt tcgggaccgc cggcctgccg
     3481 cacatcctga tgcggttcta cacggttccc gacgcgaaag cggcacggaa gtcggtattc
     3541 tacgcgaccg ggctgatcgg cgcgttctac ctgatgacgt tcgtgctcgg gttcggggcc
     3601 atggtgctcg tcggaccgga tgcgatccgt caggtggagc cggggggcaa catggcggcc
     3661 cccctgctcg ccgagttcct cggtggccgg gccttcctcg ggttcatcgc ggcggtggcc
     3721 ttcgccacca ttctcgccgt ggtggcgggg ctgacgctgt cgggcgccgc ggcgctgtca
     3781 cacgacctgt gggtgagcgt cgtgcgacgg ggtcgcgccg acaccgcgga gcagctcacg
     3841 gtggcgcgcg tggcgaccgt gggactgggc atcctcgccg tgctgctcgg catcacgttc
     3901 aagggtcaga acgtggcctt catggtcggg ttggcgttcg ccatcgccgc gagcgcgaat
     3961 ttcccggcgc tggtgctctc gatgttctgg cgagggtaca cgaccgccgg ggccgtgacg
     4021 agcatggtcg tgggcacggt atcgaccctg gtgctcatct ggctctcgcc caccgtccag
     4081 gtcgacgtgc tgaagcatcc gacggcctgg ttcccgctca agaacccggc gctcgtcacg
     4141 atcccgctgt ccttcgcggt cggcatcctg gcctcgttca tcgcggcgcg gacccacgag
     4201 gcagaagcct acggccgcat ggagcggcag aaacacctcg gcggcgcggc ctagaagagc
     4261 gtgagattga ggccggccac cagctggaac gacgaaccgc tgtgattgag cacccggccc
     4321 tccacgtagg gatgggtcat ccccagccgt gactcgagcc cgaacgggag atcccagccg
     4381 gtatcggtgt cggagacacc tccggccgac gcccgcagga agttgatgcc gcctccgacg
     4441 tacagcaccg acgggccgcc cgtcggcacc cggaacttga gatctccgct gaagccgacc
     4501 agcgagcccg cgtccacgaa gtagtagtcg aacgacgggt acagctccac cgaccgcccc
     4561 agcggcagca gcagctgcgc gccgatgagc gcccggtcga cgtcgaaatt gtatccgaca
     4621 tgcggcccga tgtgcgaccg ctgggcgtgc gccgtggccg tcgtggtggt gagcagcacg
     4681 ctcaccgcga gtgcaaccgc gacaacaggc ttcatgatcc cctcccgttc gagaatgtct
     4741 cggccgcgac gtaccctcgc cccagccgaa caactccacc acgaggaccc cccctgcgcc
     4801 gggaggttcc gccttgtgcc gtggatcacc ggcgggccga ccaccggctc cgggtcgcgc
     4861 atcacggcag gcccggtcgc gccggaccga catgtcccga gccggctcat gcgagcgcga
     4921 ggctattatt gcggaacact ttttcacccc cggagcttcg catgcgtgcc agcaatcctg
     4981 cgacctggtt ggtcctgtcg gcgctctcgc tcttcgtccg cccgacggcc gcccagactc
     5041 ccgtcgattc gttccggctc gcgttcgacc tcggatacgt gaacactgcg ggcaacaccg
     5101 ccgtgaccac gctgaacttc ggcgaacacc tgagctacct cacggggaag tggacgttcg
     5161 cgcacgggct gatcgccatc gaggggcggt cggagggagt ggaaacggcg gcacagtacc
     5221 ggacggatct tcgcgtcgat cgggcgctgg ccgcgaggct cggcgcctac gtactcgggg
     5281 gatacgagcg caacgtcttc gccgggatct cgcgccgcta ccaggggggc gctgggctca
     5341 cggccaagct cctggcgcaa ccgcgcgatc ggctggacgc ggaggccggc ctcgggctcg
     5401 tgcagcaacg caacacggcg cgcgtggacc agtcgttcgg cgcgggacgg gggccctgtc
     5461 ctacaagcac agcttcacgg agtcggcgct gctgcaggaa gtgctcgagc tgctgtcgaa
     5521 cctcaaggac tccaaggacc agctcctcaa ttccgaaacg tcgctcacgg cgccgctctc
     5581 caagcgcatc gccctgaagg ccgcgtacct gatccgctac gacaaccagc ccgagccggg
     5641 cttcaagaag tccgatcgcg tgttcacgac gggcatccag atcctcttct aggagactct
     5701 ccgatgcgtc gcctgctgcg cgccctgccg ctggtcgccg ccgttccgct ggccggccag
     5761 cggcccgccc gcccgcatgt ggaggtcccg acgatcgcgc cgcgtcccga ggacgtggcg
     5821 accctcgacg gcatcctcca ggcgttctac gacgtcatct cgggaccggc cggccagccg
     5881 cggcaatggg gccgcgaccg gacgctgtac atcccgaacg tgcgcttcgt gtcgctctcg
     5941 accgattcca cgggcgcggc gcatgcctcc gtgatggacc atcagaccta cgtggaccgg
     6001 actaacgacg cgttcgtgcg ccgcggtttc ttcgagaccg aaatccaccg caccacccac
     6061 cgcttcggca acgtcgtaca cgtgttcagc acgtacgaga tgcgcgaacg gctcgacgga
     6121 ccggtgatcg gccgcggcat caacagcgtc gagctgttct gggacgggac gcgctggtgg
     6181 atcgccgtgg cgcaatggga cgacgagcgg cccgacaacc cgattccccc ggagtacctg
     6241 cccggatcgt gatcctgtcg ctcggcgagc ggcggcgcta ctaccgggcc gcgctccgct
     6301 ttgcgcgcct ggcgcggcgg gtcggcgcga agcgcattgc ggcgggtttc cggacccgcc
     6361 tcaagcgcga cgccagtttc gtgacggagg tcgatctcgc ggtggagcgc gccgtgcggg
     6421 ccgccatcgg ccgagcgttc ccgcggcacg gcgtgttcgg cgaggagctg ccaccgagcc
     6481 atccggacgc cgagttccag tggatcctcg acccgatcga cggcaccacc agcctcaaga
     6541 acggcatccc gttctacggg accatcatcg cgctgcatca ccggggacgc ccgctcgtcg
     6601 gcgtgatcga cctgcccgcc ctggaccggt gctacagcgc cgcggcggga ctcggcgcgt
     6661 ggcgcgacgg acggcggctg cgcctgcgcg atggggcacc gcgcgacctg ccgcgcgaag
     6721 tgatctcggt cggggaccgg acgcgcttcg tgcaatgccg cgcgggcgcc aggttcgatc
     6781 gactgatgcg ggagcacggc caggtgcgtg ggtacatcga ctgcatcgcg cacgccttcg
     6841 ccgccgaagg agtgatcggc gccgccgtgg actacggggt gaagttgtgg gatatcgcgg
     6901 cgacgcagct gctggtcgag gaagcgggcg gccgttacgt gtgcgcctat cgctcgcgta
     6961 ccaaggatcc cgtgctctac ggtatcgtct gcggcaaacc cgcggtggtg cggtggctga
     7021 tgcgccggta cttcggctag cgcggaacct ggctggtgcg cccgacctcc cgcaggaact
     7081 cccgcgactt gcgcacctcc tccaggtatt cgccgatctc gtcgtcctcg cgggtggaat
     7141 cccaaccgag cagctcggcc atcttcttgg cgatggtggc cgccaccccg gcgccgtgtt
     7201 gcgggtcctc gtagaagagg tggagccggc ggatcaggac gtcgctcagg cgcatcgcca
     7261 tttcccgttc cacggcgtac acgacctcgg cccggatttc cggacggccg gtcaccaggg
     7321 gtccgcccaa gacccggttc ttggcgacga gattcaggat ggcggcggat tcggtgccgt
     7381 aactgtccac caggtggcgc gcggtcgtcg ccggcagctc cttcatgcgg gccgactgca
     7441 tcatcacgtc gagcgcggcg atctcgccgc cggggagcgg catcgcttcg gtcgctgggg
     7501 gacggggcag ggggcggccg tccacctgcc gcagccgccg ggccacgaca tccacgacgt
     7561 cgcggcccat gacctggaag gtcgtgagct tgccgccggc gatggtgacg aggccgctcg
     7621 gactttccac gatgcggtgc tcgcgggaca ctctcccggc ggggatgtca cgctcgggag
     7681 cgagcagcgg gcgcagcccg gcccaggtgg cgatgacgtc ggcgggggcg agacgcgcgc
     7741 gggggaagac ggcgttcgcg gagcgcagca ggtaggtcac atcgtccccg gtggcgcggg
     7801 ccgcctccgg cgcgacgtct tcctcggtct cggtcgtccc gatgtatgcg aggtcgttcc
     7861 aggggaggag gaacatcacc cggccgtcga tcggtgaggc gagggtgatc gcgtcgcgca
     7921 ggcccaggcg ctcgcgccgc acgatggcgt gagccccctt ggagagccgc atcagcggtt
     7981 tcacgttggg atcgtcgagc cgccgcaccc gatcgaccca gggaccggtg gcgttcacga
     8041 tgctcaacgc ccggacgatg cgttcctctc cggagatggc atcgcgcagc accgcgccga
     8101 tgatcttgcc ctcggcccgc acgaagctcg tgatcgcggt gtagttggcg cacagcgcgc
     8161 ccgactgggc cgcgccgcgc agcgcggcga cgacgagacg cgcgtcgtcc gtctgggcgt
     8221 cccaatacac ggccgcgccc tggaggtcac ggtcccgcag ccccggctcg gcctccagcg
     8281 ccttgcgcgg cgagagccag cgatggcggt gcgtgttgcg gaacgaggcc agcgcgtcgt
     8341 acagccacat gccggcccgc agcttccagg gcggcacccg cccgccgcgg tacaggggaa
     8401 agaggaatcg cagcgggcgg accaggtgcg gcgcgatgtg cagcagcacc cgccgctcgc
     8461 gcgaggcttc cagcacgagg ccgaactcgt attgctcgag gtagcgcagg ccgccgtgaa
     8521 tcagccgcga ggagcgggag ctcgtgccgg cgccgaagtc ccccatgtcg accagcgccg
     8581 tacggaaccc gcgcagcgcc gcgtcgcgcg cgatgcccgc gcccgtgatc ccaccaccga
     8641 tcacgaggag gtccaccggc tgcgactgga tcgactccag cgccgcggcg cgggtgcgat
     8701 gtgagaacgg cgcttcgccc gccaactccc ctccggatcg gcgcggtgcg cggccgcgcg
     8761 gttgcgcgat gcgaccgccc atcgcgtgcc ctgcgtcacg gggtcccggg gccggctcca
     8821 ctccacgcgg gctgaccccc ccgacatgcg ggcgtgcgcg tgaagaatgt aatcgcggcg
     8881 gaggacttcg gcgccgcacg gcgcgcgcat tcccctccgg tgcggggtca tggtaggttt
     8941 ctccgtcctt gcgtgggtgg gattggaggc ggtgcgatgg cgtttcccaa tggacggcgt
     9001 gtggcgatcg tggcggggtg ccggacgccg ttctgcaagg cggggacggt gctgaaggac
     9061 gcgcgcgccg tggatctcgc ccgccacgcg gcgcgagagc tgatcgagcg caccaatctc
     9121 gatcccgcga aggtcgacga ggtcatcttc gggcaggtcg tcgcttcacc gctggttccg
     9181 aacatcgcgc gcgaagtgag cctcctcccg cagttcccga aggaagtccc gtcgttctcg
     9241 gtcaaccgcg cgtgcgcgtc gtcgaaccag gctatcaccg ccgcccacga tcagatcgcc
     9301 caggggcacg ccgacgtcat tctggcgggc ggcgccgaag ccctctccgc catcccgatc
     9361 ctcgcctccc ggcgactctc cgacatcctc gtctccgcca gcaaggcccg cacgctgggc
     9421 gcgcggctcg ccacgttcgc gcggatccgc ccccgggacc tcatccccgt cgcgcccgcc
     9481 atcgccgaac cgtccaccgg ccagtcgatg ggtcagtcgg ccgagaagat ggcgaaggag
     9541 aatcacatcc cccgggacgc ccaggatcgc tggacgctgc gcagccacca gctggcggcg
     9601 aaggggacgg ccgacgggcg cctcaccgcg gagatcgcgc cgtggctgcc tctgcgagcc
     9661 ggcggcgaag tcgtgcaggc cgacaacggc atccgcagcg acacgacgct cgagcagatg
     9721 gccaagctga agccggtgtt cgaccgccgc tacggcagcg tcacggccgc caattcctcg
     9781 ccgcttaccg acggtgcctc ggcggtgctg ctcatggccg aggacgtggc gcgcgccctc
     9841 ggctatgagg cgctcgccta catccgctcc tacgccgtgg cggcggtgga tcccggctgg
     9901 cagctgctgc aggcgccggt ctgggccgtc cccaaggcgc tcgagcgagc ggggattcaa
     9961 tggaaggatc tcgggctgat cgagatccac gaggcgttcg cctcccaggt gctgtcgaac
    10021 ctgcagggat tcgccgagaa ggggtgggaa atcaacgagg agatcatcaa cgtgatgggc
    10081 ggctcgatcg cgatcggtca ccccttcggg gccaccggcg gacggctctt caccacgctc
    10141 tcgaatgaga tgaagcggcg cgacgtccag ttcggtctca tctccgtgtg cgcgcagggg
    10201 ggcatggcgt cggccgtcgt gctggagcgg cgctgatggc cgcgatcacc tgggaggtca
    10261 aggacggggt cgccgtcgtc acgctggact gtcccggcgc cccggtgaac gtcatctccc
    10321 aggtggtgaa ggacgaagtc tacgccatgc tgctcgccct ggagcgtgac ccccaggtgc
    10381 gcgccgtcgc gttcttctcc ggcaagaccg acaacttcat cgccggcgcc gacatcgagg
    10441 agttcgtccg gctcaagagc gccgccgagg cggagcagct gtcgcgtgac ggccaggcga
    10501 tgctcgaccg cgtggcgcag ttctccaagc cgatcgccgt cggcgttcac ggcgcgtgtc
    10561 tgggcggcgg actcgagttc gcgatggcgt gccactaccg cgtcgccacc gatcacccca
    10621 agacccagct cggtctgccc gaggtgcagc tggggattct ccccggcgcg tccgggtgcc
    10681 agcgcctgcc gcgactcatc ggcgcgcggg ccgcgctcga catcattctc gccggccgca
    10741 gcgagcgggc ggcgaaggcg ctccggctcg gaatggtgga cgagctcgtc ccgccgtcca
    10801 ttctcaagga cgtcaccatc gccgccgcgc ggcgcatggc cgaccggggc gtcatgctga
    10861 ggcgccggcg cggcgggttc atgggcctgc tgctcgacgg caatccgctg ggccgcctgc
    10921 tcgtgttccg tctggcgcgc aaacaggtct tgtcccagac gaagggcaac tacccggctc
    10981 cgctggccgc cctcgaggct gtacgtcacg gcctcaccca cggcatgccg gcggggctgc
    11041 agcgcgaggc ccggctcttc ggcgagctcg ccgtgggcga cgtgtcgcgc aagctggtgc
    11101 agatcttctt cgccacgcgg gatctgaaga aagacttcgg cgtggctgct gctccgccgc
    11161 cggcggaggt gcgccggctg gggatcatcg ggtccggctt catgggttcc ggcatcgcgg
    11221 ggacggcggt ggtccaggcg ggcgtcgacg tccgcctcaa ggactccgac ttgcctcgtg
    11281 tggcgaaggg gatcctggcc gcgcgcgcta tcgtggacga acggctcacc cggcgtcgca
    11341 tctcccgcta cgagcacgcc cgactggtcg cgttgctctc gggcggcgac agctacgcgg
    11401 gcttcggcgg cgcgggtctc gtgatcgaag cggtgttcga ggagctcgag gtgaagcggc
    11461 aggtgctgcg cgaatgcgag gcagtgctgc cggcgggagc cgtgttcgcg acgaacacct
    11521 cgaccatccc catcggccgg atcgccgagg tggcgcgccg ccccgagcaa gtcatcggca
    11581 tgcacttctt ctccccggtg gcgcggatgc cgctgctcga ggtgatcccc tcgggatcga
    11641 ccgcgccgca gacggtgagc accgccgtgg ggttcgggcg ccgcctgggc aagacggtga
    11701 tcgtcgtgcg cgatgcgccg ggcttttggg tgaaccgcat cctcggcccc tacatgaacg
    11761 aagccgcgca cctcctcctg tcgggggtcg gcgccgagga catcgaccgc gccatggtgc
    11821 gattcgggtt ccccgtcggg ccgatcacgc tgctcgacga ggtcggactg gacgtcgccc
    11881 agcacgcggg cgaggtgctg gagggcgcgt tcggcgagcg gctcgcaccg cccaaggcgc
    11941 tcgcggcgct ggtcaaggac gggcgcctcg gccgcaaggc cggcaagggg ttcttcacct
    12001 accggggggg gaagaagggg ccggtcgatt cgggcgtcta cccgctgctc ggcgttcagc
    12061 ccaacggcgg catcccgacg gccgacatcg tccagcggct cacctacgcc atgctcaacg
    12121 aagcggcccg cgcggccgcg gagggagtcg tgcgcacgcc gcgcgacggc gatatcggcg
    12181 cgatctacgg cttcggcttc ccgccgttcc gcggcggccc actgcgctac ttcgacgacc
    12241 agggcgccga tcacgtctac gccgatttgt cgcgtctcgc cgagcgcctc ggttcccggt
    12301 tcgagccctg cccggcgatc gtcgacctgg cgcgcgcccg caagcggttc tatccctgat
    12361 gctgcgcgcc ggtctgctct ggctgagcga gcaaccccgc atctcgcgtt tcgtccgccg
    12421 caaccggctc gccgggagac tcgcggggcg cttcgtggcg ggcgagacgg tggaccaggc
    12481 gctcgcggcg ctgcgcgacc tcaacgcggc ccacatctcg gccaccgtcg atctgctggg
    12541 ggaatcggtc cggcggcccg aagaggcgcg ggcggcgcgc gatacctacc tgcaactcct
    12601 cgaccgcatc cacgccgctt ccgccgacgc caacgtctct gtgaagctca cgcagatggg
    12661 attggatatc gacgaggcgc tgtgcgtgga gaccctgcgg gccattctgc agcgggcccg
    12721 ggagcacggg tccttcgttc gcatcgacat ggaagggtct ccgtacaccc agaagacgct
    12781 cgatctgttc acccgcaccg tctttccgga gttcggtcag acggccggcg tggtgctgca
    12841 gtcctatctc cgccgcacgg ccgccgacgt ggaggcgatg atcctgctgg gcgcgcgggt
    12901 ccggctctgc aagggcgcct acaaggaacc cgctacgatc gcgttccccg acaagcggga
    12961 tgtagacgcc aactacatcg cctgtctcga gcggctgctc gaccggggca gttatccggc
    13021 gatcgccacc cacgacgagc gcatcatcac gcacgcccgc gcctatgcgc gcggccgcgg
    13081 cgtcgatccg tcgcggttcg agttccagat gttgcacggc gtgcggcggg atctgcagca
    13141 ggagctgcgg cgcgcgggat atcgggtgcg cgtgtacgtc ccgttcggca gccactggta
    13201 cccgtacctg atgcgccggc tcgccgagcg gcccgccaac atcgggttca tgctcggcaa
    13261 catcttccgg gagtcgctac ggcggcgctg agcggcgacg acacgacgct gggcggctat
    13321 ctccgggtgc atcagcgtcc tccggccttc agcggctcgg acggagcggc gtactcggtc
    13381 gacgcgctcg tcgatgacga tcccgacccg gccggccggt tcggcgccgc actgctgttc
    13441 atccgctttt cggagggtgg tgagcgcgcc atcggccacc tggagacgga ctacctggca
    13501 ttcgggtcga cgccggaagc ggcgcgcgac cccgtgctcg cgctgaccct gcaggaggtc
    13561 aaggcgcatc tcgatcgctg catcgctcga aagggagtgg ccggcggatg accaccgggg
    13621 tcgtgctggc acgggcgggg agcaggttcc gggtgcacac ggcggatggc gagcgcatag
    13681 cggtgctgcg tggcaggctc aaactccccg acgacgaccg gctcgtcgcg ggcgacgtgg
    13741 tggagctcgc cctccacggc gacgggccgg cgacggtcga gcgggtattc ccgcgccgca
    13801 gcgtgctggc gcgccgccgt gcgacgggcg gccacggggc acgccgtgcg cagccgatcg
    13861 ccgccaacgt ggaccaggtg gtggtggtcg ccgcggcgcg cgatcccgag cccagcgcga
    13921 ggatgctgga tcgcttcctc gtcatcggcg aagcgaacgg cctgcccgcg attctcgtgg
    13981 tcgacaaggt ggaattggac cggtccgtga tcggcctgct gcagcagcgc tacgcccacg
    14041 ccggctatca agtcctgtcc acgtccgtga aggtgccgga agggctgccc gcgctgcgcg
    14101 atctgttgcg cgggcgggaa tcggtattca ccggcgcgtc cggggtgggg aagtcgagcc
    14161 tcttgaacgc tctcgagccc ggcctcaagc tgcgcatcgg agccatcagc gagaagtggc
    14221 gcaccggcac ccacacgacc cgggcggccg agctggtgcc gctggcactc ggcggatacg
    14281 tggtcgacac acccgggatg cgggaggtgg gcacgtgggg catcgcgccc gacgagttgg
    14341 gggcgtgctt ccctgagttc cgtccgttcc tggacgggtg ccgtttcgac aactgccgcc
    14401 acctggcgga gccgggctgc gcggtgcggg aggcggcgcg gaccggccag gtggaccccg
    14461 accggctggt gtcctacgag cggatctacg aggagatcag cgtttcctcc tggtccagcg
    14521 ctcggcgtcg cgggtcgcga ccttccgcat gaccgccgcg aacgcctcgt ccagatcgat
    14581 gccttgcgag ttcgcgagac agatcaccac gaacagaatg tcgccgagct ccatcgcgag
    14641 atcgccttcc gcttcctccg ccttcttggg cttttctccg aaccgatggt tgatctcgcg
    14701 ggccagctcg cccacctctt cgctcaagcg ggcgaggttc gtgagcgggc tgaagtagcc
    14761 ttccttatat tgactgaccc agcggtccac cgccgactgg gcttcgcgaa acgccacggg
    14821 agtctccgtc gccagaatga gtgccgacac gccgctcgtc acgccgttca tcggtgagcg
    14881 ctatgccgac ggggccgcgc tctcccgtct catcgcacct ccgtacgaca tcatctccca
    14941 ggaagcgcgc cggcggtacg cggccacgga cccgcacaat atcgtccatc tgattctccc
    15001 ggaagccgcg ggcgacgtga accgctaccg tgcggccgcc gagcacctgg ccgcgtggcg
    15061 cgcggagggc gtgttccgcg ccgattccgc tccgtccgtc tacgtcgtgg cccaggcctt
    15121 ctcgacgccc gccggcgagc gcctcacgcg cgtcggcatg ttcgccgccg tctcggcgga
    15181 gccgtacgag acgcgccgca tccgtcccca cgagaagacg caccgcggac cgaaggtgga
    15241 ccggctcgac ctgctgcgcg cgacccatgc caatctcgaa tccattttcc tgctcgcgcc
    15301 cgatcatggg gggacgttgg cggatgggct ccgggccgtg gcggaaggtc cgcgcccgcc
    15361 cgacgcctcg gcggagctgg acggcgtcca gatccgcctg tgggtccagt cgggcgccga
    15421 ggccgaccgc ctgtccgcgg cggcggcggc gccgctctat atcgccgacg gccaccatcg
    15481 ctacgagacg gcggttgcct acgccggcga gaacccggcg gcacgccggc tcctcgcctt
    15541 cgtcgtctcg gcacgcgacc ccggcctcgt cgtcctgccg acgcatcggg tggtctacgc
    15601 cgcggaccgc gacgtctccg cactcgcagg cgcctggcag cggttcttcg aagtccggcc
    15661 ggtcccgccg cccgcggacc gcgtccgtct cctcgaggag gccgggcgcg ggcgcgtcgc
    15721 ctgtatcgtc gccacggagg gggggcagca cgaccttctg ctgacgctgc gggacgacgc
    15781 accgctcgac gacatcgtgg agctcggccg caccccggcg gtgcgcggcc tggacgtcgc
    15841 gatcgtggag cggctcgtcg tcggcgagat cctgggggcg gggaagtcca cgccgaccct
    15901 cacctacacg cccgatccca ccccggcgct gcaggtcgcg catctgggga aggcgctcgc
    15961 cgccgtcctc ctcaatgcga cgaaggtgga gcaggtcttc gcggtcgccg acgcgggcga
    16021 tgtgatgccg ccgaaatcga cctatttcat cccgaaggtg ccgagcggct tggtgctccg
    16081 ccgcgtggac gcctgacggt atattccgct tccatgccgg gccgcccgcg cgaacgcatc
    16141 ctcgccaatc tcgaaacggt ctaccgcgaa gcctacgacc gcgcgaaagc cgcgggcgac
    16201 gagcgtcgca tgctcgatct cgacgctgct tatcagcgcg agcagctgct gctcgaagtc
    16261 ctgctcgaca tccgcgacgc cctccaggcc gcccccgttc cccgtgaagc cgccccctcc
    16321 cagggcgtcg atccgttggc ggcgctcgac accatccgcc gcgtcaccaa gctccgttga
    16381 ccaggagaat ttcccagcat gcgccacgcc ctccctgccg ccggcctctc cgtgctgctc
    16441 gtcgccgcct gtggcggccc caaggcagat accgtcgtgg tgaacggtat ggtctggacg
    16501 gggctgtcgt cgggggaggc acagccggga gccgtcgcga tccgcgcagg gaagattctc
    16561 gccgtgggcg attccgcgga cgtggcccgg accgtcgggt cgcacacctc ggtcatcgat
    16621 gcccacggcg gactggtcct gccggggttc gccgatgggc atacccattt cgtgcagggc
    16681 ggcttccagc tcgcctccct cgacctgcgt gatgccgcga cgcccgagga attcgtacgt
    16741 cgcatcgcgg cgttcgccaa aacgcgccag ccggggcaat ggatccaggg cggagactgg
    16801 gaccacacac tctggcgcgg gcagccgctg cctcgtcgtg agtggatcga ctccgtgacg
    16861 ccggacaacc ccgtc
//
LOCUS       DBNGZW010000005         2965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1090963, whole genome shotgun sequence.
ACCESSION   DBNGZW010000005 DBNGZW010000000
VERSION     DBNGZW010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1090963"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /locus_tag="AC16JC_00165"
     CDS             <1..770
                     /locus_tag="AC16JC_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163137.1"
                     /translation="GALSSSEPDTKTVSAGANDVDILQTATVTVAAQRPPATIAHTLL
                     TSGHDVVNQRTYITGSISPAPSALVTVAVLTHQSSSAAPPPLLSGGGMTSWEVVATVA
                     YNGTTPLDRLTIYRAMSPAPGSGPITILSSVTVSNCQWIVSQWTGVDLSGTNGSGAIA
                     QTASASGTAVTTLTAALGDFAGATNVAYGAFGVANNTAVIVPGVGFATIDQQPSGEST
                     LGDLFAEWAVLLPGVSATWPSKNAGALGVEIRASVGP"
     gene            832..1557
                     /locus_tag="AC16JC_00170"
     CDS             832..1557
                     /locus_tag="AC16JC_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007742760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HIR5163138.1"
                     /translation="MRGAALGTRVARVNAIDWTTTYAHLDEHGWAPLEGILPPDTCRD
                     IAGWYDDDTLFRSHVVMARHGFGRGEYKYFRYPLPGVIADLRTALYAGLAPHANRWND
                     ALGSDVRFPEQHTDFLERCHQAGQTRPTPLLLRYDAGDYNCLHQDLYGEHVFPFQVAF
                     LLSEPAQDFTGGEFVLVEQRPRMQSRVEVVPLRRGDGVIFAVHHRPVRGGRGVYRVNL
                     RHGVSRLRRGRRHTLGVIFHDAT"
     gene            1570..2040
                     /locus_tag="AC16JC_00175"
     CDS             1570..2040
                     /locus_tag="AC16JC_00175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5163139.1"
                     /translation="MKGAKVLQRLATAWEALLDSYAGLSEAELLEPGVTGAWSVRDII
                     AHVTWWEEEALTHLPLILTGGRPPRYSVTYGGIDAFNARMTDQRKSLPLNEVLRRRDD
                     VHRRLITYLKSVPEDELGSETRLRRRLRLDTYGHYPKHARAIRNWRDGRMALRA"
     gene            complement(2054..>2965)
                     /locus_tag="AC16JC_00180"
     CDS             complement(2054..>2965)
                     /locus_tag="AC16JC_00180"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPL family radical SAM protein"
                     /protein_id="HIR5163140.1"
                     /translation="VGCEFGCSYCYAQFTHRYVVERAHDAGRLSDAELLEFRGPHGWE
                     AFEQRIFVKQQILGALEADLRRYHRTAHSQPDGAAPIVIGTATDCYQPAERRFRITRA
                     VLGRLAACEGLSVGIITKSPLVVRDTDLLARLQQRNDLEVHISLTTLDVELIRALEVR
                     SPMPAVRLRALARLTAAGVNAGLLIAPVLPGITDDRQRLDALFAAARAAGARFVHASP
                     LRLYPAIRERFLPLLEARFPHLAARYRRAYAGRGSAPREYAAALSRRIRALQEKYGFA
                     IRGMQERYEKHIPAPQWSLALDAGKAR"
BASE COUNT          478 a         1004 c         1039 g          444 t
ORIGIN      
        1 gcggcgccct cagctcgagc gaacccgata cgaagacggt ttcggccggg gccaacgacg
       61 tcgacatcct gcaaacggca acggtcacgg tggccgcgca gcgtccgccc gcgacgatcg
      121 cgcacacatt gctcacgtcg ggccacgacg tcgtcaacca gaggacctac atcacggggt
      181 ccatcagccc cgcgcccagc gccctcgtga cggtcgcggt gctcacccat cagtccagct
      241 cggccgcgcc gcctcctctg ctgtcgggcg gagggatgac gagctgggag gtagtcgcca
      301 ccgtggccta caacggcacg accccgctcg accggctgac gatctaccgc gccatgagcc
      361 cggcgcccgg cagcgggccg atcacgatcc tgtcgagcgt cacggtctcc aactgtcaat
      421 ggatcgtgtc gcagtggacc ggcgtcgatc tgtcgggaac gaacggatcg ggtgcgatcg
      481 cccagacggc gtcggcgagc gggacggcgg tgacgaccct gacggccgcg ctcggcgact
      541 tcgcgggcgc caccaacgtc gcctacggcg cgttcggcgt cgcgaacaac accgcggtga
      601 tcgttccagg cgtgggcttc gccaccatcg atcagcagcc ttcgggagag agcaccctag
      661 gagacctgtt cgcggaatgg gccgtgctcc tgcccggagt gagcgcgacg tggcccagca
      721 agaacgcggg cgccctggga gtggagatca gggcgagtgt gggtccctga ggccggggac
      781 gatccggggc gccgctcagg catgtgcgcg agtttgacag caagaaccgg gatgcgcggg
      841 gcggcgctcg gcactagggt tgctcgtgtg aacgccatcg actggacgac gacctacgcc
      901 cacctcgacg agcacggttg ggcgccgctc gagggcatcc tgccgcccga cacgtgccgg
      961 gacatcgccg ggtggtatga cgacgacact ctgttccgca gccacgtggt catggcgcgg
     1021 cacgggttcg ggcggggcga gtacaagtac ttccgctatc cgctgccggg cgtcatcgcg
     1081 gacctgcgca ccgcgctgta cgccggcctt gccccgcacg cgaaccgctg gaacgacgcc
     1141 ctcggtagcg acgtgcgctt tcccgagcag cacaccgact tcctcgagcg gtgccaccag
     1201 gccggccaga cccggccgac gcccttgctg ctgcgctacg atgcggggga ctacaactgc
     1261 ctgcaccagg acctgtacgg ggagcacgtc ttccccttcc aggtggcgtt tcttctctcg
     1321 gagccggcgc aggacttcac cggcggggag ttcgtgctgg tcgagcagcg gccccggatg
     1381 cagtcgcggg tcgaggtagt gccgctgcgt cgtggtgacg gtgtgatctt cgcggtgcac
     1441 caccgcccgg tccggggcgg gcggggagtg taccgggtca atctgcggca cggggtcagc
     1501 cgcctgcggc gcggccgccg gcacaccctg ggcgtgatct tccacgacgc gacttgacgg
     1561 gacggacctg tgaagggagc gaaggtcctg caacggctcg ccacggcctg ggaggctttg
     1621 ctggactcct atgccggtct ctcggaagcg gaactgctgg agccgggagt caccggcgcc
     1681 tggtccgtcc gggacatcat cgcgcacgtg acctggtggg aagaagaggc gctgacgcat
     1741 ctgccgctga tactcaccgg cggacggcct ccacgctatt ccgtgacgta cggcggcatc
     1801 gacgcgttca acgcccgaat gaccgaccag aggaaaagcc tgccactcaa cgaggtgctg
     1861 cggcggcggg acgatgtcca ccgccgattg atcacctatc tcaagagcgt gcccgaggac
     1921 gagctcggga gcgaaacgcg cttacgccgc cgcctcaggc tcgacaccta cggtcactat
     1981 ccgaagcacg cgcgggcgat ccgcaattgg agggacggga gaatggccct gcgcgcttga
     2041 agtcttgggg gttctacctc gccttcccgg catccagagc cagactccac tggggagccg
     2101 ggatgtgctt ctcgtaccgc tcctgcattc cccgtatcgc gaacccgtac ttctcctgta
     2161 gggcgcggat ccttcgcgag agcgccgccg catactcgcg cggggccgac ccgcggcccg
     2221 cataggcgcg gcggtagcgc gccgcgagat gcgggaatct cgcctcgagc aacggcagga
     2281 agcgttcccg gatcgcaggg tagaggcgca gcgggctcgc gtgtacgaag cgggcgccgg
     2341 ccgcccgcgc ggccgcgaac agcgcgtcca gtcgctggcg gtcgtccgtg atgcccggga
     2401 gcaccggtgc gatcaggagt cccgcattca cgcccgcggc ggtcagcctg gccagggccc
     2461 ggagtcgcac ggcgggcatg ggagaacgga cctcgagcgc gcggatcagc tcgacgtcca
     2521 gggtcgtgag cgagatgtgg acttccagat cgttgcgctg ttggagacgg gccagcagat
     2581 cggtatcgcg cacgacgagc ggactcttcg tgatgatgcc gaccgacagt ccctcgcacg
     2641 cggccagccg tcccagtacg gcgcgcgtga tccgaaaccg ccgctcggcg ggttgatagc
     2701 agtcggtggc agtgccgatc acgatcgggg cggcgccgtc gggttgcgaa tgggcagtgc
     2761 gatgatagcg ccgcaggtcg gcctcgagcg cgccgagtat ctgttgcttg acgaagatgc
     2821 gctgctcgaa cgcctcccag ccgtgcggcc cgcggaactc gagcagctcg gcgtcgctca
     2881 gccgtcccgc gtcgtgcgcc cgctcgacca cgtagcggtg ggtgaactgc gcatagcagt
     2941 agctgcaacc gaactcgcac ccgac
//
LOCUS       DBNGZW010000006         7453 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_954746, whole genome shotgun sequence.
ACCESSION   DBNGZW010000006 DBNGZW010000000
VERSION     DBNGZW010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7453)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7453)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7453
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_954746"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..688
                     /locus_tag="AC16JC_00185"
     CDS             <1..688
                     /locus_tag="AC16JC_00185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR5163141.1"
                     /translation="DEPLALRRLRRLLRDEVDVKIVGTCGDVPAARTAIATSNPDLLL
                     LDVQLPGADGLSLLDGLPDGPRPAVIFVTAHDRYAVPAFGHEAVDYLLKPIDPERFRT
                     ALARARRRLARPERADDGAPLARLLVRERGRAFFVRVDEVDWFESAGNYVRVHLGRAT
                     HRVRLPLARLEARLDRRAFRRISRSHIVRLDGIRELQPWFHGDGLVLLSSGAKLRLSR
                     RYRDRLYETA"
     gene            complement(685..1416)
                     /locus_tag="AC16JC_00190"
     CDS             complement(685..1416)
                     /locus_tag="AC16JC_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163142.1"
                     /translation="MSRIALPVIVAALTGALGAGCGPDQATTPAFSNSQLPTFPSDAG
                     GFVSRIDNPYLAFAPGRVFHYRSETADGVETNVVEVTNQTRRILGITATVVHDQGYLD
                     GALVEDTFDWYAQDTLGNVWYLGEDSRQIENGVVVGTEGSWEAGVNGASAGLIMLAQP
                     KVGTQYQQEFAAGVAEDMAKVLSLSATAEVVYGAFTGCLKTEEWSPLEPGAREQKFYA
                     PGVGLVLELEPRGGGVRNELTAIEN"
     gene            1619..3148
                     /locus_tag="AC16JC_00195"
     CDS             1619..3148
                     /locus_tag="AC16JC_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015349235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR5163143.1"
                     /translation="MSLWATKSITALRAEAEAAGDRSLKRTLGPLNLVTLGIGAVIGA
                     GIFVLTGQAAALHAGPAVPISMALVGIACAFAGLCYAEMASAVPVAGSAYTYVYATMG
                     EFVAWIIGWDLVLEYAAGAAGVGVGWSGHFVSLLDQFGLHLPQALASSPTAWCTAAQV
                     GTMGAPGCAHAGLNWTGAIVNLPAVVIVALMSTVLVIGIRESARVNNVIVMLKVAIIL
                     LVVVVGLSHITPSNWKPFVPPNRGEWGTFGWSGVLRGAGLVFFAYIGFDAVSTTAQEA
                     KNPQKDMPIGILGSLAICTVLYMIVSAVLTGMVPYTQLNLPAPMAYAMEQIGAPWLVR
                     VSVDVGAVLGLGSVILVMLLGQSRVFFSMSRDGLLGRWAGKVHPRLRTPYLSTIYTGV
                     AVAVATGLLPLQLLGQLVNIGTMLAFVLVSAGVWILRHKRPDLERPFRTPLVPFVPIM
                     GILTCLVLMLTLPADTWIRLVVWLLIGFVIYFGYSRKHSKLQQQRRLEASSVGVKHAS
                     A"
     gene            3167..4189
                     /locus_tag="AC16JC_00200"
     CDS             3167..4189
                     /locus_tag="AC16JC_00200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR5163144.1"
                     /translation="MTSSAVPIPPERVSAARDIAAILEPGLKVALTTHVNADGDGVGS
                     ELGLWHLLRERGLRPVIANPTGIPDRFGFLLPPGADASDRAGREIARADVVIVVDISD
                     LGRLGDLATAVHDRRAPVACIDHHISPGTLPPVPRLVAPEATATAELVFDFAAALEWP
                     LSVDAARALYVGILTDTGGFRFSNTTPRALRVAAALLEHGVEPESIYEAVYASAPEGR
                     VRLLAEVLQTLVVEPERGLAWVTVPAGALERHSVTPDDLDGVVEHARSIAGVRLALLF
                     RQIANGRIKVSFRSMGSVDSAALAQGFGGGGHHKAAGASLAGSLADVQERVLDAARGY
                     LSGVGT"
     gene            complement(4206..6749)
                     /locus_tag="AC16JC_00205"
     CDS             complement(4206..6749)
                     /locus_tag="AC16JC_00205"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HIR5163145.1"
                     /translation="MLASTVLLTVAVTLQTQAVAVPSSAAASPGATRTSATRALRPPV
                     LDGRDDDEVWRLAQPITEFQEFDPDEGKPARFATVAKVAYDAHNFYAFVRACDPEPEK
                     IQRILARRDVRPPTDQLKIVIDSYHDLRSGYEFAVSPSGVKRDYAIYNDSQEDDAWDG
                     VWDVATAVDSLGWTAEFRIPLSQLRYASAPTHTFGFAVWRDIERFRERVSWPAYHRNQ
                     NTFASQLGEVTGIDGISSPRRLEAAPYVVTKNVSVPTGSGFDRDQRITAGADIKYGVT
                     SNLTLDGTVNPDFGQVEADPSVLNLSAFETFFREKRPFFIEGAGLLTFNVNCNAVNDC
                     GRENLFYSRRIGRAPQLSGLYGDATSPTASRIIGAAKVTGRSPTGLSLGLLDAVTQRQ
                     VGTGGRTIEPATNFAVARATQDFRDGATVLGVIATAVTRGNDQWSRDFLRSGALVGGV
                     DLRHRFRQKVYELDASVTGSRVTGSPTAIAATQTNSTHYYQRPDGVLGFDPTRTSLTG
                     HTEQVKFGKFSGDRLFFETSIQRVSPGYEINDLGFLRRADWQEQATWAQLHFNKPTRV
                     FQRLFWNFNEWNDWTTAGLPLEHAVNTNVHTELWNHWWLHAGGTLGQLGTTFSDRRSR
                     GGPAVRQDSYIAPWAGIEGDGRKVVVPFLWMNYWRGDEGRSTSLNLSPEIDLRVSSRW
                     TASVAMSVTRNTDNTQWTGNVTDTAGTHYSFAHLEQRTMSLQWRVNFTATPNLTVQLY
                     AEPFVSKGRYSNLRELTDPRAGSYDDRFKPFANTTVAQDFNVKQFRSNMVLRWEYKPG
                     STIFLVWSQGREDFEPGYGTRSFRGDFDELFHQHPDNTFLVKASHWFDW"
     gene            6918..>7453
                     /locus_tag="AC16JC_00210"
     CDS             6918..>7453
                     /locus_tag="AC16JC_00210"
                     /inference="COORDINATES: protein motif:HMM:NF014346.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5163146.1"
                     /translation="MSPARVVFLFATAAALELSPPAHAQGRFPPDSFKNLKVLSKSIS
                     QRALLDTMRGFAMALGVRCVYCHVGKEGQPLDSVNFAADDKRPKKVARVMMDMVMHIN
                     EEHLADVPDRPKPVVVVRCATCHRGIARPRLLDDDLALMLADSGLDATVARYRALRER
                     YYGRGSYDFGEMVLNQLAR"
BASE COUNT         1061 a         2519 c         2601 g         1272 t
ORIGIN      
        1 ggacgagccg ctcgccttgc ggcgcctgcg ccggctgctg cgcgacgagg tcgatgtgaa
       61 gatcgtgggg acctgcggcg acgtgccggc ggcgcggacc gcgatcgcga cgtcgaaccc
      121 cgatctgctc ctgctcgacg tgcagcttcc cggcgccgac gggttgtcgc tgctcgacgg
      181 gctgccggac gggccgcggc ccgcggtgat cttcgtgacg gcccacgacc gctacgcggt
      241 ccccgcgttc ggccacgagg cggtggatta tctcctcaaa ccgatcgatc ccgagcggtt
      301 tcggaccgcg ctggcgcgcg cccggcgacg gctggccagg ccggaacgcg cggacgacgg
      361 cgcgccgctc gcccggctgc tggtgcgcga gcgcggccgg gccttcttcg tgcgagtgga
      421 cgaggtggac tggttcgagt ccgccggcaa ctacgtccgg gtgcacctgg ggcgggccac
      481 gcaccgggtc cgcctgccgc tcgcccggct cgaagcccgc ctcgaccggc gcgcgttccg
      541 ccgcatcagc cggtcgcaca tcgtgcggct cgacgggatc cgcgagctgc agccgtggtt
      601 tcacggcgac gggctggtgc tcctgtcgag cggcgcgaag ctgcgtttga gccggcgcta
      661 ccgcgaccgc ctctacgaaa ccgcttagtt ctcgatcgcc gtcagctcgt ttctgacgcc
      721 gccgcccctg ggttcgagct ccagcaccag accgacgccc ggcgcgtaga acttctgctc
      781 acgagctccc ggctccagcg gtgaccattc ctcggtcttc agacatcccg taaaggctcc
      841 atacactact tcggccgtcg cggacaggga caataccttg gccatgtcct ccgcgacgcc
      901 cgcggcaaac tcctgctgat actgagtgcc gaccttgggc tgggcgagca tgatgaggcc
      961 cgccgaggca ccgttcacgc cggcttccca ggaaccctcg gtcccgacga cgacgccgtt
     1021 ctcgatctgc ctggaatcct cccccagata ccagacgttg cccagcgtgt cctgtgcgta
     1081 ccagtcgaac gtgtcctcca ccagcgcacc gtcgagatac ccctggtcgt gcaccaccgt
     1141 cgccgtgatg ccgaggatgc gcctggtctg gttggtgacc tcgaccacgt tcgtctcgac
     1201 gccgtccgcc gtttcgctcc ggtagtgaaa cacccgcccg ggcgcgaacg cgaggtacgg
     1261 gttgtcgatc cgggagacga agccgccggc gtcggacggg aacgtcggca gctggctgtt
     1321 cgagaaggcg ggtgtcgtgg cctggtcggg tccacagccg gcgccgagcg caccggtgag
     1381 agcggcgacg atgacgggca gagcgatgcg agacatgagg cacctccatt tatggttcaa
     1441 ggggaccgtg cccctgatat ctcgtgcgtg actgccgcac ggcgtgtcct ggggaagaca
     1501 ccgcccgctc cgcgaaggtc ccgcctggcc ttccaatcct cccgcccgcc ccgcaaccgg
     1561 ttcctcgcga cgtcgctcct ggcagatttc cggcaaccac aggccgggaa tccctgcgat
     1621 gtcgctctgg gccaccaagt ccatcaccgc gctgcgcgcc gaggctgagg cggcgggcga
     1681 ccgatcactc aagcggacgc tgggtccgct gaacctcgtg accctgggga tcggcgccgt
     1741 catcggcgcc gggatcttcg tgctgaccgg ccaggccgcc gcgctgcacg cggggccggc
     1801 cgtcccgatc tccatggcgc tggtcggcat cgcctgcgcc ttcgccggtt tgtgctacgc
     1861 ggagatggcc agcgccgtcc ccgttgcggg gagcgcgtac acctacgtct acgccacgat
     1921 gggcgagttc gtggcctgga tcatcggctg ggacctggtc ctcgagtacg cggcaggcgc
     1981 cgcgggggtg ggcgtgggct ggtccgggca cttcgtctcg ctgctcgacc agttcgggct
     2041 tcacctgccc caggcgctcg cgtcatcccc gacggcgtgg tgcaccgccg ctcaggtcgg
     2101 gacgatgggc gcgcccggat gcgcccatgc ggggctcaac tggacggggg ccatcgtgaa
     2161 tctcccggcc gtcgtcatcg tggccttgat gtccacggtg ctggtgatcg ggatccgcga
     2221 gtcggcgcgc gtcaacaacg tgatcgtgat gctcaaggtc gcgatcatcc tgctggtggt
     2281 ggtcgtgggc ctttcgcaca tcacgccgtc caactggaag ccgttcgtcc cgccgaaccg
     2341 gggcgagtgg ggcaccttcg ggtggtcggg cgtgctccgg ggggcggggc tcgtgttctt
     2401 cgcctacatc ggcttcgacg cggtgtccac caccgcgcag gaggcgaaga acccgcagaa
     2461 ggacatgccg atcggcatcc tcggatcgct cgcgatctgc acggtgctct acatgatcgt
     2521 ctcggcggtg ctcaccggca tggtgccgta cacccagctc aacctgccgg cgccgatggc
     2581 gtacgccatg gaacagatcg gcgcgccctg gctggtgcgc gtgtcggtcg acgtcggggc
     2641 cgtcctcggc ctgggctcgg tgatcctggt gatgctgctc ggacagtcgc gcgtattctt
     2701 ctcgatgtcg cgcgacggcc tgctcggccg ctgggcgggc aaagtgcatc ccaggctccg
     2761 gacgccgtac ctctcgacca tctacaccgg cgtcgcggtc gccgtcgcca ccgggctgct
     2821 gccgctgcag ctgctgggcc agctcgtcaa catcggcacg atgctcgcgt tcgtcctggt
     2881 gtccgccgga gtctggatcc tgcgccacaa gcgtccggat ctcgagcgcc cgttccggac
     2941 cccgctcgtg ccgttcgtcc cgatcatggg catcctcacc tgcctcgtcc tgatgctcac
     3001 gctgcccgcc gatacctgga tccgcctcgt ggtgtggctg ctcatcgggt tcgtgatcta
     3061 cttcgggtac agccggaagc actcgaaact gcaacaacag cgtcgactag aggcgtcgag
     3121 cgtaggcgtc aagcacgcct ctgcttgacg cctgttattc ttgtgcatga cctcctccgc
     3181 cgttccgatt ccccccgagc gcgtgtccgc ggcgcgtgac atcgccgcca tcctggagcc
     3241 cgggctgaag gttgccctta ccacccacgt caatgccgac ggcgacggcg tggggagcga
     3301 gctcgggctg tggcacctgt tgcgcgaacg cggcctccgc ccggtgatcg ccaacccgac
     3361 cggcattccc gaccgcttcg gattcctcct gcctcctggg gccgacgcga gcgaccgcgc
     3421 gggccgggag attgcccggg ccgatgtggt gatcgtggtc gacatctcgg acctgggacg
     3481 gctgggagat ctggcgaccg cggtgcacga ccggcgcgcg ccggtcgcct gcatcgacca
     3541 ccacatcagc ccggggacgc tcccgccggt gccacggctg gtcgcacccg aggcgaccgc
     3601 gacggcggag ctggtgttcg atttcgccgc ggcgctggag tggccgctgt cggtcgacgc
     3661 cgcgcgcgcg ctctacgtcg gcatcctcac cgacaccggc ggcttccggt tctcgaacac
     3721 gacaccccgc gccctgcgcg tggcggcggc cctgctggag catggtgtgg agccggaatc
     3781 catctacgag gcggtctatg cgagcgcgcc ggaaggacgg gtgcggctcc tggccgaggt
     3841 gctgcagacg ctcgtggtgg agccggagcg gggccttgcg tgggtgacgg tgccggccgg
     3901 cgcgctggag cggcacagcg tcacgcccga cgacctggac ggggtcgtgg agcacgcccg
     3961 gtcgattgcc ggcgtccgcc tggcgctgct gttccgccag atcgcgaacg ggcgcatcaa
     4021 ggtgtcgttc cgctcgatgg gcagtgtgga ttcggctgcc ctggcgcagg gcttcggtgg
     4081 gggcggccac cacaaggcgg ccggcgcttc cctcgcgggg tcgctcgccg acgtccagga
     4141 gcgcgtgctg gacgcggccc gaggctatct cagcggcgtg gggacctgag gcctgtcgct
     4201 ccgcactacc agtcgaacca gtgactggcc ttgacgagga acgtgttgtc ggggtgctgg
     4261 tggaacagct cgtcgaaatc gccccggaac gagcgggttc cgtaccccgg ctcgaagtcc
     4321 tcgcggccct ggctccagac caggaagatc gtggagccgg gcttgtactc ccagcgcagc
     4381 accatgttgg agcggaactg cttgacgttg aagtcctgcg ccaccgtcgt attggcgaag
     4441 ggcttgaagc ggtcgtcgta cgacccggca cgcgggtcgg tgagctcgcg cagattggaa
     4501 tagcgaccct tggagacgaa gggctccgca tagagctgca ccgtcaggtt cggcgtcgcg
     4561 gtgaagttga cgcgccactg gaggctcatg gtgcgctgtt ccaggtgggc gaaggaatag
     4621 tgcgttccgg cggtgtccgt gacgtttccc gtccactggg tgttgtcggt attgcgggtg
     4681 acgctcatgg cgaccgaggc cgtccagcgg gacgacacgc gcagatcgat ctcggggctg
     4741 aggttgagcg aggtcgagcg gccttcgtcg ccgcgccagt agttcatcca gaggaacggt
     4801 acgacaacct tgcggccgtc gccctcgatc ccggcccagg gggcgatata ggagtcctgg
     4861 cgaacggccg gcccgcctcg ggacctgcgg tccgagaagg tggtcccgag ctgccccagc
     4921 gtgccgccgg cgtggagcca ccagtgattc cacagctcgg tgtgcacgtt ggtattcacg
     4981 gcgtgctcca gcggcaggcc ggccgtggtc cagtcgttcc attcgttgaa gttccagaac
     5041 agccgctgga agacgcgcgt cggcttgttg aagtgcagct gggcccaggt ggcctgctcc
     5101 tgccagtcgg cgcggcgcag gaagccgagg tcgttgatct cgtagcccgg tgagacgcgc
     5161 tggatgctgg tttcgaagaa caggcgatcg ccgctgaact tgccgaattt cacctgctcg
     5221 gtgtggccgg tgagcgaggt gcgggtcggg tcgaacccca ggacgccgtc ggggcgctgg
     5281 taatagtgcg tgctgttcgt ctgggtcgcg gcgatcgcgg tgggcgagcc ggtgacgcga
     5341 ctgcccgtca ccgacgcatc gagctcgtac accttctggc ggaaccggtg tcgcagatcg
     5401 acgccaccca cgagggcgcc cgagcgcagg aagtccctgg accactggtc gttcccgcgg
     5461 gtcaccgccg tcgcgatcac gccgagcacg gtcgcgccgt cgcggaaatc ctgcgtcgcc
     5521 cgggcgacgg cgaaattggt cgccggctcg atggtgcgcc ctccggtgcc cacctgccgc
     5581 tgggtgacgg cgtcgagcag cccgagcgag aggccggtgg gcgagcgccc cgttaccttc
     5641 gcggcgccga tgatcctcga ggcggttggg gacgtcgcat ccccgtacag gccggacagc
     5701 tgtggggcgc ggccgatgcg ccgggaatag aacagattct cacggccgca gtcattcacc
     5761 gcgttgcagt tgacgttgaa ggtcaggagc cccgcgcctt cgatgaagaa cgggcgcttt
     5821 tcccggaaga acgtctcgaa cgcgctgagg ttcagcaccg acggatcggc ttccacctgg
     5881 ccgaagtcgg ggttgacggt gccgtcgagc gtcagattcg aggtcacgcc gtacttgatg
     5941 tcggcccccg cggtgatccg ctggtcgcgg tcgaacccgc tgccggtcgg cacggagacg
     6001 ttcttggtca cgacgtacgg tgccgcttcg agacgacgcg gcgaggaaat gccgtcgatc
     6061 ccggtcacct cgccgagctg cgaggcgaag gtgttctggt tgcgatggta cgccggccag
     6121 ctgacgcgct ccctgaatcg ctcgatgtcg cgccacacgg cgaatccgaa ggtgtgggtc
     6181 ggcgcgctcg cgtagcgcag ctgcgacaac gggatccgga actccgccgt ccagccgagg
     6241 gagtccaccg cggtcgctac gtcccacacg ccgtcccagg cgtcgtcctc ctgactgtcg
     6301 ttatagatgg cgtaatcccg cttcaccccg ctggggctca cggcgaactc gtagccggag
     6361 cggaggtcgt gataggagtc gatgacgatc ttcagctggt cggtgggcgg gcggacgtcg
     6421 cggcgggcca ggatccgctg gatcttctcc ggctcggggt cgcaggcgcg gacgaacgcg
     6481 tagaagttgt gcgcgtcgta cgcgaccttc gcgacggtgg cgaagcgcgc cggcttcccc
     6541 tcgtccgggt cgaattcctg gaactcggtg atcggctgcg ccagccgcca gacctcgtcg
     6601 tcgtcccgac cgtccagcac gggtgggcgc agcgcacggg tggcgctcgt cctggtggcg
     6661 ccgggactcg cggcggcgct cgagggcacc gcgacggcct gggtctgcag agtgacggcg
     6721 acggtcaaca gcacggtcga tgcgagcacg cgagcacccc gaggagacgg atcgaggagt
     6781 aagacagcat acggggcaaa agggttgtct gccttcagcc ggcgggtatt ctgactgatt
     6841 gcacacactc tggtcgggtg acccggccgg ccgggggata tgattgagcc cctacacctt
     6901 accaggcgag gccctcgatg tcgccagctc gcgtcgtgtt tctcttcgca accgctgccg
     6961 ccctggagct gagccccccc gcccacgccc aggggcgatt tccgcccgat tccttcaaga
     7021 acctcaaggt cctgtcgaag tccatctcac aacgcgcgct gctcgacacc atgcgggggt
     7081 tcgccatggc gctcggcgtg cgctgcgtct actgccacgt gggcaaggag gggcagccgc
     7141 tcgattcggt caacttcgct gccgacgaca agcgccccaa gaaagtggcc cgcgtgatga
     7201 tggacatggt gatgcacatc aacgaggagc acctggccga cgtgcccgac cggcccaaac
     7261 cggtcgtcgt ggtccgctgc gccacctgcc accgggggat cgcccgtccg cggctgctgg
     7321 acgacgacct ggcgctcatg ctggccgact cggggctcga cgcgacggtg gcgcgctatc
     7381 gcgccctgcg ggagcgctac tacggaaggg ggtcctacga tttcggcgag atggtcttga
     7441 accagctggc gcg
//
LOCUS       DBNGZW010000007         2330 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1022994, whole genome shotgun sequence.
ACCESSION   DBNGZW010000007 DBNGZW010000000
VERSION     DBNGZW010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2330)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1022994"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..870)
                     /gene="istB"
                     /locus_tag="AC16JC_00215"
                     /pseudo
     CDS             complement(274..870)
                     /gene="istB"
                     /locus_tag="AC16JC_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223846.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
     gene            complement(897..2183)
                     /gene="istA"
                     /locus_tag="AC16JC_00220"
     CDS             complement(897..2183)
                     /gene="istA"
                     /locus_tag="AC16JC_00220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HIR5163147.1"
                     /translation="MFVTGDSLSPAMTAGEERMETPGDVAVMLRLAELGWGSKRIAAE
                     LGCSRTTAKRYLRQGGWASCRGRGRPSALAGLEGWLAERFRRHRGNADVVRQELEREH
                     EVRVSLRTVERAVADMRRDLLAEARACVRFETPPGRQLQIDFGSTFVEIGEEVVRVHL
                     FVATLGYSRRGFVAAFRHERQTAWFEGLERAFRHFGGVPREVLLDNAKPLVVRHDAVT
                     REVVFNERLHAFARYWGFRPRACAPYRARTKGKDENGVGYVKKNAIAGHRFASWAALE
                     AHLVRWMREVADQRIHGTTGEPPIERFRREEAEALASLMGRPPFRQVREVVRRVQADG
                     SVDLDTNHYSVPWRLIGARVEVTAGVVRIFHARMEVARHDERRGRRERVLDPAHLVGV
                     IANDRDASVTSVAAPAEVGELLRPLAEYEEIAGGGW"
BASE COUNT          395 a          874 c          727 g          334 t
ORIGIN      
        1 ccaaccactt ccccgacggc accctccagg acctccaggg cctcggctac gatctgggtc
       61 tgaccggctt cgacgtcggc gagctgcgca gtctgctgga cgaggcaccc gcaacctgtc
      121 aagcgacact cagaactggc cccctggcga cactcagaac tggccctcct tggtcatgat
      181 gggctttgtc caggtagttc atgggagggt cccgcgcgga gcgcagcgcc tctcgcgcgg
      241 ctggcgtagc gaagcgcggg aggggacctg tggacccacc tgggcaaagc tgcggttgct
      301 ggtggatcat caagctgccg cgctcgtagc gcttcgaaac cagctggaag aacaggtgcg
      361 ccgcgtcggg cgcgaacggc aggtagccca actcgtcgac gatcaacagc ttcggcttgg
      421 cgtagaacgc caggcggtcc tccagccggc cctcggcatg cgccctggcc agtgtggtca
      481 gcagcgtggt cgccggagtg aacagcaccg agtagccgat cttgatcgcc tcgcggccga
      541 gcgccaccgc gcgatgcgtc ttgccgacgc cgggcggacc gagcaggagc agcgcatcac
      601 cgttggccac ccaccggcag gtcgccagat cgcgcacctg cttcgggtcg agcgagggct
      661 gggcgtcaaa ctcgaagccg tcgagcgttc tgacaaacgg gaacttggcg atgcccaagc
      721 ccatgctgat ccgccgttcc tcgcggtgcg ccacctcggc cgcgcacacc aaggagagtg
      781 ctcgcgcagg gagaaatcgc gccgcccggc ctcgtcgagc aggttgtcga gccggtcccg
      841 catcgccttc aggccgagcc gcgccagcat ctcgggcagg cggtcgagat cggcattcac
      901 cacccacctc cggcgatctc ctcgtactcg gccaacggcc gcaacagctc cccgacttcc
      961 gccggcgccg ccacggacgt caccgacgcg tcccggtcgt tggcgatgac gccgaccagg
     1021 tgggcggggt cgagaacgcg ctcacgccgg ccgcggcgct cgtcgtgacg ggcgacctcc
     1081 atcctggcgt ggaagatgcg caccacgcca gccgtcacct cgacccgggc gccgatcagc
     1141 cgccagggca cgctgtagtg gttggtgtcg agatcgaccg agccgtccgc ctgcacgcgc
     1201 cggaccacct cgcgcacctg ccggaacggc ggccggccca tgagcgacgc cagcgcctcg
     1261 gcttcctccc gccggaatcg ctcgatcggc ggctcgccgg tggtaccatg gatgcgctga
     1321 tcggcaacct cgcgcatcca tcggacaagg tgcgcctcca gcgccgccca agaggcgaac
     1381 ctgtggccgg cgatcgcatt cttcttcacg tagccgacgc cgttctcgtc cttgcccttg
     1441 gtccgtgccc gatacggcgc acaggcgcgc ggccggaacc cccaatagcg cgcaaaggcg
     1501 tgcagccgtt cgttgaacac gacctcgcgc gtcaccgcat cgtgacgaac gaccagcggc
     1561 ttggcattgt cgagcaggac ctcgcggggc actccaccga agtggcggaa cgcccgttcc
     1621 aggccctcga accaagcggt ctggcgctcg tggcggaacg ccgcaacgaa accgcggcgc
     1681 gagtagccca gcgtcgccac gaacagatgg acccgcacca cctcttcgcc gatctcgacg
     1741 aacgtcgagc cgaaatcgat ctgcagctgc cggcccggcg gcgtctcgaa gcgcacgcac
     1801 gcccgcgcct cggccagcag atcgcgtcgc atatccgcca ccgcccgctc gaccgtgcgc
     1861 agactgaccc ggacctcgtg ctcgcgctca agctcctgtc ggaccacgtc ggcattacct
     1921 cggtgccgac gaaaccgctc agccagccag ccctccaacc cggcaagcgc cgacggccga
     1981 ccccgacccc ggcacgacgc ccagccaccc tgccgcaggt accgcttcgc cgtcgtgcgg
     2041 ctacacccca attcggccgc gatccgcttc gatccccagc ccagctcggc cagccgcagc
     2101 atcaccgcca catcccccgg cgtctccatc cgctcctccc ccgccgtcat cgccggggaa
     2161 agagagtctc ctgtcacaaa catctcccct ccttctcgaa ggggggtcag ttttcatgtc
     2221 gcccgacacg aacgagcgtt ccggcgccag aagcggcggg caagcgcggg ggtcgatggg
     2281 attacggtga tggaatacga gcagaacctg gaagccaatc tccaggatct
//
LOCUS       DBNGZW010000008         1931 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1500410, whole genome shotgun sequence.
ACCESSION   DBNGZW010000008 DBNGZW010000000
VERSION     DBNGZW010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1931
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1500410"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="AC16JC_00225"
     CDS             complement(<1..356)
                     /locus_tag="AC16JC_00225"
                     /inference="COORDINATES: protein motif:HMM:NF013567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5163148.1"
                     /translation="MDDRVGVGLIGSQFISTIHAEALQSVADARVVAVMSPTAGHARD
                     LAAKYHIPNHCTALDQLLARDDIDMVVIGAPNVLHCDITLQAAQAGKHVVIEKPLCMN
                     LAEADRMIDGCRAAQV"
     gene            complement(431..859)
                     /locus_tag="AC16JC_00230"
     CDS             complement(431..859)
                     /locus_tag="AC16JC_00230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163149.1"
                     /translation="MRLTPVATTVESLAALPRPGYRPPQGRLAPTETTIFCIEGWFKD
                     VNTEADYDLHVVVAGLEDTTVTMIVEFPDARCSGACASGYAELYARARQALEDRLKTW
                     TTDTLRVRIMGVGFFDRPHGQWGGAPNSIELHPVLGIDFP"
     gene            complement(1155..>1931)
                     /locus_tag="AC16JC_00235"
     CDS             complement(1155..>1931)
                     /locus_tag="AC16JC_00235"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR5163150.1"
                     /translation="ERGDPERAARMLGAVAKLREEAGAAPLPLERESMDRVAESLRAT
                     LGAAAYAAAFKAGQVLNLGQALDQAEAMVGEPAGAARPAGTPVLRVSALGTLEIAVNG
                     EPLPASAWRGDARPRELLLYLLCHPAGRTRDQIGLACWPDASPAERKNSFHVTLHHLR
                     STLGRPDWIVFEEGRHRLNPRFVVQFDGRQFEAQVRAARAALARTRDAAPLVRALELY
                     RGDFLQDAPAGDWYLEYRDRWRRLYDEATVIREALSHSGA"
BASE COUNT          290 a          663 c          658 g          320 t
ORIGIN      
        1 ttgacctgcg ccgccctgca cccgtcgatc atgcgatccg cttcggccag attcatgcac
       61 aacggcttct cgatcacgac atgcttgccg gcctgcgccg cctggagcgt gatgtcacag
      121 tgcaggacgt tgggcgcgcc gatgacgacc atgtcgatgt cgtccctggc caggagctga
      181 tcgagggcgg tgcagtgatt ggggatgtgg tacttcgccg ccaagtccct ggcatgtccg
      241 gccgtcggtg acatgaccgc cacgacgcgt gcgtccgcga ccgactggag ggcctctgca
      301 tggatcgtcg agatgaattg cgacccgatg agcccgacgc ctaccctgtc atccatcgct
      361 gttgcctccc cgctctcggc gcgctgcacg gtgtgctgaa cggctgtctg acgactctcg
      421 actgcctggg ctagggaaag tcgatcccca gcaccggatg cagctcgata ctgttcggcg
      481 cccctcccca ttgcccgtgc ggccggtcga agaatcccac tcccatgatg cgcacgcgca
      541 gcgtgtcggt cgtccaggtc ttgaggcgat cttcgagcgc ctggcgcgcc cgggcgtaca
      601 gctcggcgta gccggaagca caggcacccg agcaacgggc gtccgggaac tccacgatca
      661 tggtcaccgt ggtatcctcg agtccggcaa cgacgacgtg caggtcgtag tcggcctcgg
      721 tgttgacgtc cttgaaccat ccctcgatgc agaagatggt cgtctccgtg ggcgcgaggc
      781 gcccttgcgg cggccggtag ccagggcggg ggagcgcggc gagcgattcg acggtcgtcg
      841 cgacaggggt cagtcgcacg cgacgcgcgt cgcggtccga cagggtcttg acctgccagc
      901 gctccgtgcc gcagtgcaag ccacagcgga ggggagccgg ccgtggctgc gccggaatcg
      961 ttacagaagg gcaggggctg cgctcggacg tcgagtctct gccggggtgc tgagcggcga
     1021 gcgagggaag agccgcaatg agcagcgcga ggacgagcgg atgcgctctc tgatgttcga
     1081 ctgcgccact ggatgcgttc tttcgatgat gatccatccc gattccttca ccggacactg
     1141 gggaccataa accgctacgc gcctgagtga ctgagcgcct ctctgatgac cgtcgcctca
     1201 tcgtatagcc ggcgccaccg atccctgtac tccaggtacc agtcgcccgc cggcgcgtcc
     1261 tgcaggaagt cgccgcgata cagctccagt gcgcgtacga gtggcgcggc gtcgcgggtt
     1321 cttgcgaggg ccgcccgcgc ggcccgcacc tgggcttcga attgccgtcc gtcgaactgc
     1381 accacgaaac gcgggttgag ccggtggcgc ccttcctcga acacgatcca atccggccgg
     1441 cccagcgtgc tccgcaggtg gtgcagggtg acgtgaaagc tgttcttgcg ctccgcgggt
     1501 gaagcgtcgg gccagcacgc gagcccgatc tggtcgcgcg tgcgtcctgc cggatgacac
     1561 agaaggtaga gcagcagctc gcgcggcctg gcgtcgcccc gccaggcgga ggcggggagt
     1621 ggctcgccgt tcaccgcaat ctcgagggta cccagtgcgc tcacccgcag caccggcgtg
     1681 ccggccgggc gagccgcccc cgcaggctcg cccaccatcg cctcggcctg atccagggcc
     1741 tggcccaggt tcagtacctg gcccgccttg aacgccgccg cgtatgccgc tgcgccgagc
     1801 gtggcgcgga gcgactcggc aacacggtcc atggactcgc gctcgagcgg cagtggggcg
     1861 gctccggctt cctcgcgcag cttcgccacg gcgccgagca tgcgcgcggc ccgctcggga
     1921 tcgccccgct c
//
LOCUS       DBNGZW010000009         2031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1636744, whole genome shotgun sequence.
ACCESSION   DBNGZW010000009 DBNGZW010000000
VERSION     DBNGZW010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1636744"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="AC16JC_00240"
     CDS             complement(<1..701)
                     /locus_tag="AC16JC_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D-related
                     protein"
                     /protein_id="HIR5163151.1"
                     /translation="MSKRTVELDVATPGVDARGEPVRVPLTMAEAPQPAEPDMGGEHL
                     LINLGPQHPATHGVLRLVVELDGETVVRVIPHLGYLHSGFEKLGEYRHYNQIIPLTDR
                     TDYLSPMANNVALALAAEKLMGVAITPRCTVLRVIACEMSRIISHMVWLGTTAIDLGA
                     FTPFLWAFQERERIYNLQEMWTGARLTTSLTRVGGMMADIPDGFVEGLRHFTKTYPKT
                     LNEIDRILTRNAIWAG"
     gene            complement(698..1318)
                     /locus_tag="AC16JC_00245"
     CDS             complement(698..1318)
                     /locus_tag="AC16JC_00245"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR5163152.1"
                     /translation="MNPTVDTLRATFGDAIGRAGEARDGLSVVVVAGARLLEVMRWLR
                     DTPGQTYDYFVDVTAVEYRDRERPMEVVYQVRSLERKLDLRVKVELDPQGELAVDSVT
                     PLWAGANWLEREVYDMFGIVFAGHPDLRRILMWETYAEGYPLRKDFPLRGRFSRAEQV
                     HQALNANPEAHYSLEELSIAEAYDEVPADMRERLRRGERGVVPESE"
     gene            complement(1315..1932)
                     /locus_tag="AC16JC_00250"
     CDS             complement(1315..1932)
                     /locus_tag="AC16JC_00250"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR5163153.1"
                     /translation="MKHVLETKEVAGSPNFHLTRFEKLVDWAVNQSRANSLWPMPFGT
                     ACCAIEFMATAASRYDLARFGMERQAFSPRQADVLICAGRLPFKLAPVIRRIFDQMPQ
                     PKWVISMGACASTGGIFDNYAMVQGIDTIVPVDVYVPGCPPRPEGLLYGILLLHKKIK
                     GESLFDAVPRRDEHPLDAHGLRLPPAEIDEISEPFGNSVHQFRSA"
BASE COUNT          314 a          690 c          703 g          324 t
ORIGIN      
        1 cccgcccaga tggcgttgcg cgtcaggatc cggtcgatct cgttcagcgt cttgggatag
       61 gtcttggtga agtggcgcag cccttcgacg aacccgtcgg ggatgtcggc catcatgccg
      121 ccgacccgcg tcaggctggt ggtgagccgg gccccggtcc acatctcctg caggttgtag
      181 atgcgctcgc gctcctggaa cgcccacagg aacggcgtga acgcgccgag gtcgatggcg
      241 gtcgtgccga gccataccat gtgcgagatg atgcggctca tctcgcacgc gatcacgcgc
      301 agcacggtgc agcgcggcgt gatcgcgacg cccatcagct tttccgccgc cagcgccagg
      361 gcgacgttgt tcgccatggg cgagaggtag tcggtgcggt cggtgagcgg gatgatctgg
      421 ttgtagtggc ggtactcgcc cagcttctcg aagcccgagt gcaggtaccc gaggtgcggg
      481 atcacgcgca ccacggtctc cccgtccagc tccaccacga gccgcagcac gccgtgggtg
      541 gcgggatgct ggggccccag gttgatcagc aggtgctctc cccccatgtc cggctcggcc
      601 ggctgcgggg cctcggccat ggtgagcggc acccgcaccg gctcgccccg ggcatccact
      661 cccggcgtgg cgacgtcgag ctcgaccgtg cgcttgctca ttcggactcc ggcaccacgc
      721 cgcgctcgcc gcggcgcagc cgctcgcgca tgtcggccgg cacctcgtcg taggcctcgg
      781 cgatcgacag ctcctcgagc gagtagtgcg cttcgggatt ggcgttcagc gcctgatgca
      841 cctgctcggc gcggctgaag cggccgcgca gcgggaaatc cttgcgcagc ggatagccct
      901 cggcgtaggt ctcccacatc aggatgcgcc gcaggtccgg gtgtccggcg aacacgatgc
      961 cgaacatgtc gtagacctcg cgctcgagcc agttcgcccc ggcccagagc ggcgtgaccg
     1021 agtccaccgc cagctcaccc tgcgggtcga gctccacctt cacgcgcagg tccagcttgc
     1081 gctccagcga ccgcacctgg tacacgacct ccatcgggcg ctcgcggtcg cgatactcga
     1141 cggcggtgac gtccacgaag tagtcgtacg tctggccggg cgtgtcgcgc agccagcgca
     1201 tcacctcgag cagccgcgcg cctgcgacga cgacgacgct gagaccgtcg cgcgcttcgc
     1261 cggcgcggcc gatcgcgtca ccgaaggtcg cccgcagcgt gtcgacggtc gggttcacgc
     1321 cgagcggaat tggtggacgg aattgccgaa gggctcggag atctcgtcga tctcggccgg
     1381 tggaaggcgc aggccgtgcg cgtccagcgg gtgctcgtcg cggcgcggca ccgcgtcgaa
     1441 cagcgattcc cccttgatct tcttgtggag cagcaggatg ccgtacagca ggccttcggg
     1501 acgcggcggg cagcccggca cgtacacgtc caccgggacg atcgtgtcga tcccctgcac
     1561 catcgcgtag ttatcgaaga tcccgccggt ggaggcgcag gcgcccatcg agatcaccca
     1621 tttgggctgc ggcatctggt cgaagatgcg gcggatcacc ggggcgagct tgaagggcag
     1681 ccggccggcg cagatcagca cgtcggcctg gcgcggcgag aacgcctgcc gctccatccc
     1741 gaagcgggcc aggtcgtagc gggaggccgc ggtcgccatg aactcgatgg cgcagcaggc
     1801 ggttccgaac ggcatcggcc acagcgagtt ggcgcgcgac tggttgacgg cccagtccac
     1861 cagcttctcg aagcgggtca ggtgaaagtt ggggctgccc gccacctctt tggtttccag
     1921 gacgtgtttc atattgaggc aacaccgtcg agcagaggcg tcgagcagaa acgtcgagca
     1981 acgacgtcaa gcgaggacgt acgacctcgc tcgacgggtt cgctcgacgc c
//
LOCUS       DBNGZW010000010         4125 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_750688, whole genome shotgun sequence.
ACCESSION   DBNGZW010000010 DBNGZW010000000
VERSION     DBNGZW010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4125
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_750688"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..458)
                     /locus_tag="AC16JC_00255"
     CDS             complement(<1..458)
                     /locus_tag="AC16JC_00255"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5163154.1"
                     /translation="MATPTKQSEMLTGADGGPLRVDVRRSGHAAATVVICHGFKGFKD
                     WGFFPVIAERLARAGFAVAAFNFSGSGVGEDGQSFSEVERFGRATYSHHLQDLDIVLG
                     ALAEGRLGFRPSRVGLLGHSMGGGIAVLRAAADPNVAALVTWAATARFGPA"
     gene            523..690
                     /locus_tag="AC16JC_00260"
     CDS             523..690
                     /locus_tag="AC16JC_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163155.1"
                     /translation="MQVELAAAFGAVGPTSYGLGDRTRVGVGSGPYWGPRKQWSESAS
                     YRLEVVFGQRG"
     gene            complement(700..1410)
                     /locus_tag="AC16JC_00265"
     CDS             complement(700..1410)
                     /locus_tag="AC16JC_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5163156.1"
                     /translation="MTSAIATVAGIGLALGLRHAFEPDHLAVVSTLATRQGSPLAAAR
                     LGLAWAVGHTASVGVVVLAIIALGIHFPASLWPVADLLVGALLIVLGGSVLYRYRRGG
                     WHLHAHSHGTGEGAHLHLHSHAASPAHGHPHPPADVRRSLAFGLLHGLAGSAAVLVLL
                     VAAAPTRGAQLAYFVAFGSGTAVGMLGVSLTLAGLVKAASHRGAGWARALHLGAAAAS
                     IVVGAVLAVTTARQLLFS"
     gene            1537..2091
                     /locus_tag="AC16JC_00270"
     CDS             1537..2091
                     /locus_tag="AC16JC_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5163157.1"
                     /translation="MQAPITDATTAAMDGEAADVALAQSGDGRAFERLYRSHVPRVHG
                     LVRRMLDSADADDVTQDVFVRAWEKLHTFRGEAAFWTWLHRLAVNVILGRRKTVGGER
                     RRFHDDAGMLDEVAGRHEAPEHGVDFEDALRRLPEGARQVFLLHDVEGYRHEEIAQLL
                     GIVPGTSKSQLHHARMALRRHLDR"
     gene            2125..2802
                     /locus_tag="AC16JC_00275"
     CDS             2125..2802
                     /locus_tag="AC16JC_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR5163158.1"
                     /translation="MSDNWTDRLSEYLDGELDSAQRAELETHLAGCDACRATLAELGR
                     VVDRARTMDERPPARDLWSGIAGRIGLSTDDLALRRARRRISFTVPQLIAAGLALIAL
                     SAGTARLVSNKASTPGTSATASASSRLSAVQAALWNKADSSADQEVDELRLALAEGQR
                     SGQLNPATVRKLEHSLAIIDSAIAEAKRALALDPQSPYLNHHLADTMRRKLDFLREAN
                     RIATART"
     gene            2817..3686
                     /locus_tag="AC16JC_00280"
     CDS             2817..3686
                     /locus_tag="AC16JC_00280"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR5163159.1"
                     /translation="MLFALVAAAGMMLNQDTDTTFAVRQGQRLDVSDFGGTVVIKSWR
                     QSSVRVRATHGSRDYVTVSAEGATVSISASSRHGPAMVDYEILAPAWMPISVSGTPSA
                     EVTIEGSEAEIAVETVEGAVTVVGGRGNIALKSVEGEVSLTGAKGHIELNSVDGSITV
                     KDCSGDITAATVDGEIGLLNIESADVDASTVDGSIEYDGTITNGGRYRLTSHDGDVSV
                     SMPERANATISVATFSGSFDSYLPVTLGPGQKTKHRFSFTLGSGGARVELESFDGSIR
                     LVRPGQSHEQDKE"
     gene            3692..>4125
                     /locus_tag="AC16JC_00285"
     CDS             3692..>4125
                     /locus_tag="AC16JC_00285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163160.1"
                     /translation="MRATVYQIPAMLAAALLAAAPAEAQDFHWSGRLAAGKRLEIRGV
                     NGSIRTVAATGDEIDVSARKTARRSDPDEVKIEVVPSDEGVTICAVYPTPSGARRDNT
                     CEPGDGWHSSTDNNDVTVDFMIKLPAGIAFSGETVNGEIDAEG"
BASE COUNT          716 a         1402 c         1446 g          561 t
ORIGIN      
        1 gcaggcccga accgtgccgt cgccgcccag gtgaccagcg ccgccacgtt gggatccgcc
       61 gcggcccgca acacggcgat tcccccgccc atgctgtgtc ccagcagtcc gacccgcgac
      121 gggcggaagc ccagtctccc ctccgccagt gcgccgagca cgatgtcgag atcctgcaga
      181 tgatgcgaat aggtcgcgcg gccgaagcgc tccacctcgc tgaacgactg cccgtcctcg
      241 ccgacccccg atccagagaa gttgaacgcc gcgacggcga atccggcccg ggcgaggcgc
      301 tccgcgatca ccgggaagaa cccccaatcc ttgaacccct tgaatccgtg acagatgacg
      361 acggtggcgg cggcgtgtcc ggatcgccgc acatccacgc gcagcggacc gccgtcggcg
      421 cccgtcagca tctcgctctg ttttgtgggt gtcgccaccg gccttcctta gtcctcactc
      481 gcccctacat tcgcctgcca ggaatctatc ggttggcgcg gcatgcaggt cgagctcgcg
      541 gccgccttcg gcgcggtcgg accgacctcc tacggtcttg gcgaccgcac ccgcgtgggc
      601 gtgggctccg gtccgtattg gggaccgcgg aagcaatggt cggagtctgc gagctaccgg
      661 ctggaagtgg tcttcggaca gcgcggctga accgcggcct taactgaata gcagctgccg
      721 agcggtcgtg accgcgagga ccgctcccac cacgatgctc gccgccgcgg ccccgagatg
      781 gagcgctcgc gcccagccgg cgccgcggtg cgacgccgcc ttcaccaacc ccgccagcgt
      841 gagcgacacc ccgagcatcc ccaccgccgt ccccgacccg aacgcgacga aataggcgag
      901 ttgggcgcct cgcgtcggcg ccgccgccac cagcaggacg aggaccgcag cactcccagc
      961 cagcccgtgt aacaggccga acgcgagcga gcggcgcacg tcggccggcg gatgcgggtg
     1021 tccgtgtgcg gggctggcag catggctgtg gagatgcagg tgtgcaccct ctcccgtacc
     1081 gtggctgtgc gcatgcagat gccagccgcc gcgccggtag cggtagagga ccgagccccc
     1141 gaggacgatc agcagcgcgc cgacgaggag atcggccacc ggccagagcg aagccgggaa
     1201 gtggataccc agggcgatga tcgccagtac cacgacgccg acactcgcgg tgtgacccac
     1261 ggcccaggcg aggccgagtc gcgccgccgc gagcggcgac ccttggcgcg tcgccagcgt
     1321 cgagacgacc gcgaggtggt cgggctcgaa cgcatgtcgc aggcccagcg ccagcccgat
     1381 gccggcgacg gtcgcgatgg cggaagtcat tgggccaatg tacgtcggaa ttctaaaggc
     1441 cgcgtgaagg cgcctggcgg cgggccggat cagattgcaa ccttggggcc ttccttgact
     1501 atccaagctc gtgaacggcg gaccgggacg aggggagtgc aagcaccgat taccgatgcg
     1561 accacggcgg cgatggacgg cgaagcggcg gacgtagcgc tcgcgcagag cggcgatggt
     1621 cgcgccttcg aacgcctgta ccgcagccac gtcccgcggg tccacggtct ggtgcggcgc
     1681 atgctggact cggccgacgc ggacgacgtg acccaggacg tcttcgtgcg ggcgtgggaa
     1741 aagctgcaca cgttccgcgg cgaggccgcg ttctggacct ggctgcaccg gctcgcggtg
     1801 aacgtgattc tgggccggcg caagaccgtg ggcggggagc gcagacgctt ccacgacgac
     1861 gccggcatgt tggacgaagt ggcgggacgg cacgaggcac cggaacacgg ggtggatttc
     1921 gaggacgcct tgcgtcgctt gcccgaaggg gcgcgccagg tctttctgct gcacgacgtg
     1981 gaggggtacc gccacgagga gatcgcgcag ctgctgggga tcgtcccggg gacctcgaaa
     2041 tcacagctgc accatgcgcg gatggcgttg cgcaggcacc tggatcgata gacgaagagc
     2101 acggagcagg agcaggagga catgatgagc gacaactgga cggatcgcct gtccgagtac
     2161 ctcgatggcg agctggatag cgcccagcgg gccgagctcg agacgcatct cgcgggctgc
     2221 gacgcgtgcc gggcgacgct cgccgaactg gggcgggtcg tcgatcgagc ccggaccatg
     2281 gacgagcgcc cgccggcgcg cgacctgtgg tcggggatcg ccgggcggat cggactctcg
     2341 accgacgatc tcgcgctgcg gcgtgcccgc cggcgcatca gcttcaccgt gccgcagctc
     2401 atcgcggccg gcctcgcctt gatcgccctg tccgccggca ccgcgcgtct ggtgtcgaac
     2461 aaggcgtcca cgccgggcac cagcgcgacc gcctccgctt cctcgcggct gtccgcggta
     2521 caggcggcgc tgtggaacaa ggcggactcg agcgccgacc aagaggtcga cgagctgcgg
     2581 ctcgcactgg ccgaaggaca gcgcagcggc cagctcaatc ccgcgacggt ccgcaagctg
     2641 gagcacagcc tcgcgatcat cgactccgcg atcgccgagg cgaagcgggc gctggcgctc
     2701 gatccgcaga gcccgtacct gaaccaccac ctcgccgaca ccatgcggcg caagctggat
     2761 ttcctgcggg aagcgaatcg catcgccacc gcacgtacat aagagaagga gcactcatgt
     2821 tgttcgcact tgtcgccgcc gcggggatga tgctgaacca ggacaccgac acgacgttcg
     2881 cggtgcgcca ggggcagcgc ctcgacgtca gcgacttcgg cggcacggtc gtgatcaaga
     2941 gctggcgtca gagctcggtc cgcgtgcgcg ccactcacgg cagccgggac tacgtgacgg
     3001 tgtccgccga aggggcgacc gtgagcatca gtgcctcgtc gcgccacggc cccgccatgg
     3061 tggattacga gattctcgca ccggcctgga tgccgatctc ggtgtccggc acgccgtcgg
     3121 ccgaggtgac gatcgaaggg tccgaggctg agatcgcggt tgaaaccgta gagggggcgg
     3181 tcaccgtcgt gggggggcgc gggaacatcg cgttgaagtc cgtcgagggg gaagtctcac
     3241 tcacgggcgc gaagggccat atcgagctca acagcgtgga cggaagcatc accgtgaagg
     3301 actgcagcgg cgacatcacg gcggcgacgg tggacgggga gatcggcctg ctgaacatcg
     3361 agtccgccga cgtggacgcc tccaccgtgg acggcagcat cgagtacgac gggacgatca
     3421 ccaacggcgg ccgctatcgt ctgacatcgc acgacggcga cgtgtccgta tcgatgccgg
     3481 aacgggccaa cgcgacgatc tcagtagcga ccttcagcgg cagtttcgat tcctatctcc
     3541 ccgtgacgct cggtcccggg caaaaaacga agcaccggtt cagcttcacc ctgggctcgg
     3601 gcggcgcccg ggtggagctc gaatcgttcg acggctcgat caggctggtg cggcccgggc
     3661 agtcccacga acaggacaag gagtgagcga catgcgcgcg acagtctatc agatcccggc
     3721 gatgctcgcc gccgcgctgc tggcggcggc accggcggag gcccaggatt tccactggtc
     3781 cggccggctc gccgccggca agcggctcga gatcaggggc gtgaacggca gcatccgcac
     3841 ggtggcggcg acgggcgacg agatcgacgt gagcgcccgc aagacggccc gccgctccga
     3901 tcccgacgag gtaaagatcg aggtcgttcc ctccgacgag ggagtcacga tctgcgccgt
     3961 gtaccccacg ccctccgggg cgcggcgcga caacacctgt gagccgggcg acgggtggca
     4021 ctcttctacc gacaacaacg acgtgaccgt cgacttcatg atcaagctcc ccgcgggcat
     4081 cgcgttcagc ggagagaccg tcaacggcga gatcgacgcc gaagg
//
LOCUS       DBNGZW010000011         2997 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1296054, whole genome shotgun sequence.
ACCESSION   DBNGZW010000011 DBNGZW010000000
VERSION     DBNGZW010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1296054"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..812
                     /locus_tag="AC16JC_00290"
     CDS             <1..812
                     /locus_tag="AC16JC_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR5163161.1"
                     /translation="SRQPADTPGANQYCSRFCCTATVHTSIQVAGLDPSIRQFHLYRD
                     MRTYGKFEPLYTESREAGSVFLKYAQEAPPAVSRAAGGRLTVTVHDLLTENAELAIPV
                     DLVVLVTGMVPRRNEALASVLKLPAGSDGFFNEIHPKLRPVETVVDGVLIAGACQGPK
                     TSSESVGSGLAAVTQSAAVLKKGFTELDPLVAIVHADACSACGACLTACPYDAISMGE
                     GGTRHIALISPTGCKGCGGCVPLCPENAIDLLGYTDDQVTSMIDSLVEVPA"
     gene            812..1057
                     /locus_tag="AC16JC_00295"
     CDS             812..1057
                     /locus_tag="AC16JC_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HIR5163162.1"
                     /translation="MSPTLRDVREIVREEPLMRPRILAALRDGPRTVPEIAEAIGAPA
                     SEVVIWVMGLRRYGWLAEVKGATTDSYFQYGPTGRAS"
     gene            1057..2259
                     /locus_tag="AC16JC_00300"
     CDS             1057..2259
                     /locus_tag="AC16JC_00300"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5163163.1"
                     /translation="MTASGLASTAITTVDLGLYPELQRYGATDVSACFSCGTCTASCP
                     LSQTDSTFPRRIIRYAQVGMKDALLTSKELWSCYQCGECAETCPTQADPSEFMAAARR
                     YAIASYDRTRIARTMSTRPLLGTAFAVLVAMFFALFMYSAHGPQSGESLALFTFIPEG
                     LIHDTGVIVMILVALAGLSGIASMARGIARREGVGWRSLVGSRAALARSARAAWSAVA
                     HESLAQDRFRRECETDAEPLPWYRRRWLLHAVTVWGFLGLFAATLLDYGLALIGIKAT
                     GTPVPIWYPVRLLGTLAGVLLLYGTTTLIVQRHLAANRSVKRSTSADWMLLSLLWITG
                     ATGFVLELALYLPSAPSWGYWIFLVHVVVAMELMLLAPFMKLAHAVYRPVALFFVALA
                     QSSAEGDA"
     gene            2355..2849
                     /locus_tag="AC16JC_00305"
     CDS             2355..2849
                     /locus_tag="AC16JC_00305"
                     /inference="COORDINATES: protein motif:HMM:NF013936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HIR5163164.1"
                     /translation="MTETLPRVAHEHHERLLQHVDRMPEIGDVILTGDAVEVRRVAHE
                     TSTFLTDTLVPHVDAAERSLYPELERMLQNRHSMGPMRREHGEVRRLVADFARLTQKL
                     DAGSIALGKRLALRRVLFQLYALLKIHLAEEEAYLRVVDHGVSSEVADLLAAALDHPG
                     FQSA"
BASE COUNT          419 a         1072 c         1024 g          482 t
ORIGIN      
        1 gcagtcgtca gccggccgac acgcccggcg ccaaccagta ctgctcgcgt ttctgttgca
       61 cggcgaccgt ccacacctcg atccaggtag ccgggctgga cccgtcgatc cgccagttcc
      121 acctctatcg cgacatgcgg acctacggca agttcgagcc gctctacacc gaatcgcgcg
      181 aggcgggatc ggtcttcctc aagtacgcgc aggaagcgcc gccggcagtg tcccgcgccg
      241 cgggcggccg gctgacggtc acggtccacg acctgcttac cgagaacgcc gagctcgcga
      301 tccccgtcga cctcgtggtc ctggtcacgg gcatggtgcc gcgccggaac gaggcgctgg
      361 caagcgtgct caagctcccc gcgggatcgg acgggttctt caacgagatc cacccgaagc
      421 tgcgcccggt cgagacggtc gtcgacgggg tgctcatcgc gggcgcctgc cagggcccga
      481 agacgtcgtc cgagagcgtc ggatcgggtc ttgcggcggt cacccagagt gccgccgtcc
      541 tcaagaaggg ttttaccgag ctcgacccgc tggtcgcgat cgtccacgcg gacgcctgca
      601 gcgcctgcgg cgcgtgtctg actgcgtgcc cgtacgacgc gatctcgatg ggcgaggggg
      661 ggacacgcca catcgcgctc atcagcccga ccggctgcaa gggttgtggc ggctgcgttc
      721 cgctctgccc ggagaacgcg atcgacctgc tcggctacac cgacgaccag gtcacttcga
      781 tgatcgacag cctggtggag gtgcccgcct gatgtccccg acgctgcgag acgttcgcga
      841 gatcgtgcgc gaggaaccgc tgatgcgccc ccgcatcctg gccgcgctgc gcgatggacc
      901 gcggacggtc ccggagatcg ccgaggcgat cggcgcgccg gcgtccgagg tcgtgatctg
      961 ggtcatgggc ctgcgccgct acggctggct ggccgaggtc aagggcgcga ctactgacag
     1021 ctacttccag tacgggccca ccgggcgagc ttcctgatga ccgcctccgg gctggcctcc
     1081 actgcgatca cgaccgtcga cctcggcctc taccccgagc tccagcgcta cggcgcgacg
     1141 gacgtcagcg cctgcttcag ctgcgggaca tgcaccgcct cgtgtccgct gtcgcagacc
     1201 gactccacct tcccgaggcg gatcatccgc tacgcccagg tcgggatgaa ggacgcgctg
     1261 ctcaccagca aggagctgtg gagctgctac cagtgcggcg agtgcgccga gacctgcccg
     1321 acccaggccg accccagcga attcatggcg gctgcccggc gctacgcgat cgccagctac
     1381 gaccggacgc ggatcgcccg gacgatgtcc acgcggccgc tgctcgggac ggcgttcgcc
     1441 gtgctcgtgg cgatgttctt cgcgctgttc atgtattcgg cgcacgggcc gcagagcggg
     1501 gagtcattgg cgctgttcac gttcatcccg gagggcttga tccacgacac gggggtgatc
     1561 gtgatgatcc tcgtcgcgct ggccgggctg agcggcatcg cctccatggc ccgcgggatc
     1621 gcccgccgcg agggcgtggg ctggcgcagc ctggtcggaa gccgcgcggc actcgcgcgc
     1681 tcggcgcgcg cggcgtggtc cgccgtggct cacgaatcgc tcgcccagga tcgcttccgc
     1741 agggagtgcg agaccgacgc cgagccgctc ccctggtatc ggcgccggtg gctgctccac
     1801 gcggtcacgg tgtgggggtt cctggggctg ttcgccgcga ccctgctcga ctacggcctg
     1861 gcactcatcg ggatcaaggc aaccggtacc ccggtgccca tctggtaccc ggtgcggctg
     1921 ctgggcacgc ttgccggcgt cctgctgctg tacgggacga ccacgctcat cgtccagcgc
     1981 cacctggccg ccaaccggtc ggtcaagcgt tcgacgagcg cggactggat gctgctctcg
     2041 ttgctctgga tcaccggcgc gaccggcttc gtcctcgagc tcgcgctgta cctgccatcg
     2101 gcaccgtcgt ggggctactg gatcttcctg gtccatgtgg tcgtggcgat ggagctcatg
     2161 ctgctcgctc cgttcatgaa gctggcccac gccgtctatc ggcccgtggc cttgttcttc
     2221 gtcgcactcg cgcagtcgtc cgcggagggt gacgcataag tggatccgtc gcgtggtacc
     2281 gggtgtcccg cccgagcttc gggacccgtc cagccggccg tgagcggccg gcgacaccgc
     2341 cgaggaggcg agccatgacc gaaaccctgc cgcgggtggc gcacgagcac cacgagcggc
     2401 tcctgcagca cgtcgaccga atgcccgaga tcggcgacgt gatcctgacc ggcgacgccg
     2461 tcgaggtgcg acgcgtggcc cacgagacca gcacgttcct gaccgacacg ctggtgccgc
     2521 acgtcgatgc cgccgagcgg tcgctctacc cggagctcga gcggatgctc cagaaccggc
     2581 actcgatggg gccgatgcgg cgcgagcacg gggaagttcg ccggctggtc gccgatttcg
     2641 cgcggctgac ccagaagctc gacgccggat cgatcgcact gggcaagcgg ttggcgctcc
     2701 gtcgggttct cttccagctg tacgccctgc tcaagatcca tctcgcggag gaggaggcct
     2761 acctgcgcgt cgtggaccac ggcgtgtcca gcgaggtcgc cgacctgctg gccgcggccc
     2821 tcgatcatcc ggggttccag tccgcctgac gcggctcagg cgcctggtcg ccgtgcccga
     2881 cgccgacgca ctatgctccg ggagcatatg agtgcgcgcg cctctcagcc gccgcccgat
     2941 tcagacccgg cgctcgcgga gccggctgcc ccggccacgc ccggtgctgg ccagccc
//
LOCUS       DBNGZW010000012         2587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1364238, whole genome shotgun sequence.
ACCESSION   DBNGZW010000012 DBNGZW010000000
VERSION     DBNGZW010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1364238"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..>2587
                     /locus_tag="AC16JC_00310"
     CDS             38..>2587
                     /locus_tag="AC16JC_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5163165.1"
                     /translation="MSGTPGSGPITVTFSKSQSNCQWIVSQWAGVESGGVNGAGAIGQ
                     VGSNGGDAVNGLAVALGVFANSGNVAYGVFGVNRNAPAISPGSGFAEIAEVPSGENTG
                     ADLEAEWATNRNSIAGDWSALNGGALGVEILAGGLPPPVVSVEVTPSQASVPRSGTVQ
                     LTATPRDADGNPLPGRPVTWESSDAAVATVDTAGLVTGVALGTATIIATSEGQSGTAT
                     ITVLPVPETPVDSVAIAPLEASTAIGETVQFTATPLDSAGHALPGKTVTWTSSDTGVA
                     SVTNTGLAAGVGAGSATITAMSEGKIASAAIVVAAVRVATVDVEPASVPLQVGATAQL
                     TATPRDALGNPLSGRVVTWTTSDVSIAFVDGSGLVTGAREGAATVSATSEGRSGSSAI
                     TVTAQPSCLDRAGPLLTLSGVQTNVFDDRELAADTRIDASTAQFLTGTSKAIRVGGGS
                     RSCLRGGEVIGQLPPSTDWVTMHGTYAIQVEGLPFFTLEGMREFNFGDGITLSDSSRN
                     WSIKGVYFKYMRDDCVQNDWLNSGTIDSSLFDGCSTGLSSRPYATTVDGSDNVIVVKN
                     SLFRLQYMDQGYEAPGHGGFFKWAETGPMVSLFNNVYRADSPSSLGGHTLGPPAGKLK
                     DCANNVMIWLGSGPFPEPLPACYTLLTGAAGLAYWDNAVATWKANHPSALADIGPPIV
                     SIVTPLEGASLEAIVTLTATAVDDRDVAGVQFRIDGRDIGSEVKAESLLTRFIMSWDS
                     RAEANGTHVITAVARDAAGKTTTSAGITVTVANGAASLSTVTASPDTLPAGDSVSTIT
                     VTVLDASGAPMSGIPVTLSATGSGNSIVQPGPSGADGVTTGTLSSTVAGDKT"
BASE COUNT          435 a          866 c          903 g          383 t
ORIGIN      
        1 cgccactccg cacaagcgcc tcacgatcta tcgggccatg agcggtaccc cgggcagcgg
       61 gccgatcacc gtcacgttct ccaagtcgca gtcgaactgt cagtggatcg tctcgcagtg
      121 ggcaggcgtg gagagcggcg gcgtgaacgg cgccggcgcc atagggcagg tcggatcgaa
      181 cggcggtgat gccgtgaacg gcctggcggt cgccctcggc gttttcgcca acagcggcaa
      241 cgtggcctat ggcgtattcg gcgtgaaccg gaatgcgccg gcgatctccc cggggagtgg
      301 cttcgcggag atcgccgagg tgccgtcggg cgagaacacg ggcgccgacc tcgaggcgga
      361 gtgggcgacc aaccgcaact cgatcgccgg cgactggagc gccctgaacg gtggagccct
      421 cggcgtcgag atcctggcag gcggcctgcc accaccggtg gtgtctgtgg aggtcacccc
      481 ctcgcaggcg agcgtgccgc gaagtgggac agtgcagctc acggcgacgc cgcgtgacgc
      541 ggacgggaat ccgctccccg gtcgcccagt gacgtgggag agcagtgacg cggccgtggc
      601 tacggtcgat accgccgggt tggtaacggg ggtcgccctc ggtacggcga ccatcatcgc
      661 aacgagcgaa gggcaaagcg ggacggcgac gattaccgtc ctgccggtcc cggagactcc
      721 ggtcgactcc gtcgcgatcg cgccgctcga ggcgagcacg gcgatcgggg agacggtcca
      781 gttcacggcc actccgctcg actcggcagg ccacgcgctc ccggggaaga cggtcacctg
      841 gacgagcagc gacacgggcg tcgcctcggt cacgaacacc ggcttggccg cgggcgtggg
      901 cgcgggctcc gccacgatca ccgccatgag cgaagggaag atcgccagcg cggcgatcgt
      961 ggtagcggcg gtgcgcgtgg ccacggtgga cgtggagccc gccagcgtcc cgctccaggt
     1021 cggggcaacc gcgcagctca cggccacccc cagagacgcg ctcggcaacc cgctgagcgg
     1081 ccgggtcgtg acctggacga caagcgatgt gagcatcgcg ttcgtcgatg gatccggtct
     1141 cgtcacggga gcgcgtgaag gcgctgcgac ggtcagcgcg acgagtgaag gacgcagcgg
     1201 cagctcggcg atcacggtga ccgctcagcc gtcctgtctc gaccgcgccg gtccgcttct
     1261 caccttgagc ggcgtccaga cgaatgtctt cgatgaccgg gagctggcgg cggatacgcg
     1321 catcgacgcg tccacggcgc agttcctcac cgggacgtcc aaggccatcc gggtgggcgg
     1381 cggctctcgc agctgtctcc ggggtgggga agtgatcggg caactcccgc cttccacgga
     1441 ctgggtgaca atgcacggca cctatgcgat tcaggtggag ggccttccgt tcttcacact
     1501 cgaggggatg cgcgaattca acttcgggga tgggatcacg ctctcggaca gctcccggaa
     1561 ctggtcgatc aagggcgtct acttcaagta catgcgggat gactgcgtcc agaacgactg
     1621 gttgaactcc ggcacgatcg actcctccct gttcgacggc tgttccactg gactgagctc
     1681 gaggccctat gcgacgacgg tggacggcag cgacaacgtg atcgtcgtca agaactcgct
     1741 gttccgtctg cagtacatgg accagggcta cgaggctccc ggtcacggcg gcttcttcaa
     1801 gtgggccgag acggggccga tggtctccct gttcaacaac gtgtaccggg cggacagccc
     1861 gagcagcctc ggcggccaca cgctcggtcc gcctgcgggc aagctgaagg actgcgcgaa
     1921 caacgtcatg atctggctgg ggagcggccc gtttccggaa cccctgcctg cctgctacac
     1981 gctgctgacc ggagcagccg gccttgccta ctgggacaat gccgtcgcca cctggaaggc
     2041 gaatcacccc tcagcgctgg ccgacatcgg gcccccgatc gtgtccatcg tcacgccgct
     2101 cgaaggcgcg tcgctcgaag cgatcgtcac tctcacggcg accgcggtgg acgaccggga
     2161 cgtggccgga gtccagttcc ggatcgacgg aagggacatc ggatcggagg tgaaggccga
     2221 gtcgctcctc accaggttca tcatgtcctg ggactcgagg gccgaggcga acggcacgca
     2281 cgtcatcacg gcggtggcgc gcgacgctgc cgggaagacg acgacctccg ccgggatcac
     2341 ggtgacggtc gccaacggcg ccgcgtccct ctccaccgtt acggcctccc cggatacgct
     2401 gcccgcaggc gacagcgtct cgacgatcac cgtcaccgtg ctggacgcct ctggagcccc
     2461 gatgagtggc ataccagtca cgttgtcggc cacggggtca ggcaactcca tcgtgcagcc
     2521 cgggccctcc ggggccgacg gcgtcaccac gggcacgctc agctccacgg tcgccggaga
     2581 caagacg
//
LOCUS       DBNGZW010000013        10905 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1501205, whole genome shotgun sequence.
ACCESSION   DBNGZW010000013 DBNGZW010000000
VERSION     DBNGZW010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 10905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10905
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1501205"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="AC16JC_00315"
     CDS             <1..890
                     /locus_tag="AC16JC_00315"
                     /inference="COORDINATES: protein motif:HMM:NF024260.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HIR5163166.1"
                     /translation="WCTETAPARELSPRIMPSAEHLVSYHIITEGRCYGGLAGAEPVE
                     LVAGDVIVFPHGDAHVLSSGPDGAAARAVLTTAPARYPFTSFFGAQEPRSVSIVCGFL
                     GCDRRPFNPLLAALPRQVHVRGASNAWLGSFVRQASEETRLGRPGAEGVLTRLAELMF
                     IEVLRRHLADLPAGHTGWLAGLRDELVGRALALIHGRAGHPWTLAELARETGASRTTL
                     TRRFALLVGQPPMQYLTQWRMQVAANQLSQGAAKVAAIGADVGYDSEAAFSRAFKKAT
                     GLAPGAWREARRPARLTNP"
     gene            1015..1554
                     /locus_tag="AC16JC_00320"
     CDS             1015..1554
                     /locus_tag="AC16JC_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5163167.1"
                     /translation="MSTKTIPAVRRPGDHGAAPISRRAEALAVRLEQGASALASLAST
                     LTEAEWQTRIPRDGRKVGVVVHHVGNMYPIEIEAALTLAAGNPVVGVTADVINEINAR
                     HAQEFDAVTKDEALEFLRRNSATAAAAIRALSDEQLDRAATISLNADAPLTCQFWLED
                     HPVRHSYHHLAKIRAALGR"
     gene            complement(1642..3009)
                     /locus_tag="AC16JC_00325"
     CDS             complement(1642..3009)
                     /locus_tag="AC16JC_00325"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020652255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase family protein"
                     /protein_id="HIR5163168.1"
                     /translation="MRPNSGVSTIAVLLVAIAPTRGGAQARDTLLSALEEPLRAGFAP
                     LVPAAPGHPVLLRPDRVFDATSEAAHTGWVVLVQGDSIAAVGSADAVRIPRGTETIAL
                     PGLTLLPGLIDAHSHLFLHPYNETLWNDQVLKEPEGYRVVEAVLHARNTLMQGFTTLR
                     DLGTEGAGFEDVSLRRAIVEGLIPGPRLQVATRAIVATGSYGPGPRGFRPDLDLPQGG
                     QAVSGVPQVLEAVREQAGRGADWIKVYADYGRGPGGAAVPTFSQEELDALVAEAHASG
                     RPVAAHASTPEGMRRAILAGVNTIEHGFGGTDSLFHLMAERGVAWLPTLTAVEAYAEY
                     FEGYVRGRDPTAGMRQAAQAFQLALKNGVTIGCGSDVGVFTHGANARELEWMVRDGMR
                     PAQALLAATAVDARILGMEHEVGRVAPGLKADLVAVAGDPTRDIAVVSRVRFVMKNGV
                     IYRAP"
     gene            complement(3042..5264)
                     /locus_tag="AC16JC_00330"
     CDS             complement(3042..5264)
                     /locus_tag="AC16JC_00330"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR5163169.1"
                     /translation="MHLSRRLLAVAFLGGLISTPLRAQSDSLRIARLTALGKLFGVVK
                     YFHPAFLDHDVPWDSAVVVAIDAVGAARSADDYRTAVANLLGVLGDPATRISAGNASE
                     VRSAGTTSPSQRWMVSGTDSTLVIAIPDFDNYVAAMQLLNSAAADVRRAPGLVFDLRS
                     PEPEELGNSSFVFANTIQGALATTAISAPAQRLRMHSGFVPQLGGTSGGYWSGSYTRD
                     GGVIRGSSNNRLRRIVFLVTPGTDIPEVAFALRNAGQGAIVAEGADAQLRAGSETYDV
                     QMGEGVTATVRLSEVVGPAGADTVVSHGAVGAGDAALDVAIAFAKRPVTAPLFPRVPS
                     AHVPAADDAYPGMRSPSAAYRILAAYRYWNTIHYFYPYKHLIGEDWADVLPRSIRMLE
                     AARDSLELGMALATMVTYTHDSHSGVIRNTLLSAYFGVLPAAVQLQYIGGQPVVIRVG
                     DDSATKASGIAAGDVVVKVDGEDVVARRARIARFVASSTPQALDNAIAQRLLRGTDSV
                     ATLIVRGRDNRDRALVLPRRQSFDRLMQYPRSGPVLQVLTGNVGYADLSLLTPAMVDS
                     MFELFKNTKGIIFDGRGYPQGTAWSIAPRLSAAPVPAAAFQRPLVMSPDSAAWTTYNF
                     IQSTPPGSKWVYPGKTILLVDERTISQAEHTGLFLEAANRTTIVGSPTMGANGDVTTV
                     ALVAGMYATFTGHDVRHADGRQLQRIGLQPDVVVRPTLAGIRAGRDEVLERALAILKN
                     "
     gene            5446..6228
                     /locus_tag="AC16JC_00335"
     CDS             5446..6228
                     /locus_tag="AC16JC_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163170.1"
                     /translation="MKPTIYAPILGLVALAACNSLNSTGYTAPPPPAYTTYETLGDSV
                     SIATKLDEFRAALGGALNAPNAPPAASGRREINWDGVAAALTNVDNFPADFFNVNSKR
                     GAVFATPGTGLRIDSTNFAAINAGLGAQFKFFSPKKTFMAVGSNQVDVDFKLVGTTTN
                     GLVNGFGVVFSDVDRLGSTTVELFDANDVLIAVLRAPNHSGAQLLSFTGAVFASPIIA
                     RARITSGDAALNGTILDLSAGGTADLVVMDDFVYGEPQPLPQ"
     gene            6264..6647
                     /locus_tag="AC16JC_00340"
     CDS             6264..6647
                     /locus_tag="AC16JC_00340"
                     /inference="COORDINATES: protein motif:HMM:NF012355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5163171.1"
                     /translation="MRYTTWMTAAALLALAACGGVTSPGGGGGGGGPVGDITVGNIFF
                     RSVHNGTQNPAVDTIAAGSSITWAWNAAGSHSIRSTGTPGIFRNSVVMSGASDTYTVT
                     FNTPGTYTYDCAVHGSAMTGRIVVQ"
     gene            6759..7898
                     /locus_tag="AC16JC_00345"
     CDS             6759..7898
                     /locus_tag="AC16JC_00345"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR5163172.1"
                     /translation="MPSALPQPPEFNTPALLEAFFERSQDGFFFMMLDEPIEWGPGVD
                     KDAALDYVFSHQRMTKVNPAMAQQFRARPAELIGLTPGEFFRHDLAAGRRGWRALFDA
                     GHTHSITNERRLDGTTMWVEGDYMCFYDATGRITGHFGIQRDVTDRTLAAKELEQSRA
                     ELRALAARLQTTREEERTRIAREIHDELGQALTALKLDLAWLGTKRPRGNSGALRLTD
                     KSITTRIDETMEVVRRIASELRPSVLDQLGLDAAIEYLVQETTKRTGIAVTLHAEEFP
                     KLPDDVASHAFRIIQEALTNVARHSKATRVDVTVRRLEAAIILGVEDNGVGFKPQSLS
                     GLRSLGLVGMRERALACGGALTIRGNPGEGTAIVVTIPVAAARAD"
     gene            7901..8533
                     /locus_tag="AC16JC_00350"
     CDS             7901..8533
                     /locus_tag="AC16JC_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012526107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5163173.1"
                     /translation="MIRILIADDHPIVRQGLRRIVEADAGLVISGEVGDAAALLSAVQ
                     TTPTDLVLLDVSMPGGSFLETLQNLRARHPAIRVLVLSVHPEDQWAVRALRAGASGYL
                     TKDHSPDQLLEAIRRVYRGGKYVSPALAEQLASQFGGGEQQSLHELLSDREFEVMRRL
                     GSGLTVSQIAGELKLSAKTVSTYRKRILDKMAVTTNADLVSYAARHGLIG"
     gene            complement(8564..9055)
                     /locus_tag="AC16JC_00355"
     CDS             complement(8564..9055)
                     /locus_tag="AC16JC_00355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163174.1"
                     /translation="MGQHRLPRPSKDSWWGYDELHIEDPDGNDSIVTAPDDAPSPTGR
                     PLSVTVVGWLFVAAGSVGLVYHATELRAPGPFDSEVIWVLLVRLAAIVGGAFVLRGAR
                     WASWLLVAWMAYHVVLSALHSWSEAAVHAVLLVGVAYLLLRPEASAYFRRPSRVTGRS
                     SDA"
     gene            complement(9737..10270)
                     /locus_tag="AC16JC_00360"
     CDS             complement(9737..10270)
                     /locus_tag="AC16JC_00360"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR5163175.1"
                     /translation="MPTPLSHAAVGYALGAWIQPGRPSARVCLAAAACAALPDIDAIG
                     WVLHIADTSTIAHRAITHSLTFALVSALLTTAFFFRRGLWMQYRTRIGITLAVASISH
                     SCLDALSTYSLGVEFLAPFSHHRFRFPWTPLGGLHASLAAQLAQETVVVLLPALLAAW
                     LGFRIRRRGISPNPAAA"
     gene            complement(10426..>10905)
                     /locus_tag="AC16JC_00365"
     CDS             complement(10426..>10905)
                     /locus_tag="AC16JC_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR5163176.1"
                     /translation="SIYKYFAVEPQILYVRKGTELIVPPCGPGASCPSTVDYRQDYVE
                     LPLLFTGTYPIAGRLRFAPTVFAGPALAFQTSCTFSSSGPIAVASCDSLYATEGASMR
                     HTDALLIFGGGFNVGPFTLLARHDLGLTKLVVQRSPQDVKSEAWLLSAGVSLRFGKR"
BASE COUNT         1874 a         3666 c         3677 g         1688 t
ORIGIN      
        1 cgtggtgcac ggagacggcg cccgcccggg agctctcgcc gcgcatcatg ccgtcggccg
       61 agcacctggt ctcctaccat atcatcaccg aagggcgctg ctacggaggg ctcgccggag
      121 cggaaccagt ggagctcgtc gccggcgatg tgatcgtctt cccccacggc gatgcccacg
      181 tgctgtccag tgggccggac ggcgcggccg ctcgggccgt cctgaccacc gcgccggccc
      241 gctatccctt cacgtccttc ttcggcgcgc aggaaccgcg gtccgtttcg atcgtctgcg
      301 gattcctggg gtgcgaccgc cgtccgttca acccgctgct ggcggcgctg ccgcgacagg
      361 tgcacgtgcg cggcgcgtcc aacgcctggc ttggcagctt cgtccgccag gcgagcgagg
      421 aaacccgcct cggccggccc ggggccgaag gcgtgctcac caggctggcc gaactgatgt
      481 tcatcgaggt gctgcgtcgc catctcgcgg acctgcccgc tggacacacc ggctggctcg
      541 ccggactgcg tgatgagctg gtcggccggg cgttggcgtt gatccatggc cgagccgggc
      601 atccctggac cctcgccgaa ctggctcgag aaacgggggc ttcccggacc accctcacca
      661 ggcgattcgc gctgctcgtc ggtcagccgc cgatgcaata tctgactcaa tggcggatgc
      721 aggtcgcggc caaccagctg tcgcagggcg cggccaaggt cgcggcgatc ggcgccgacg
      781 tgggttacga ctccgaggca gcgttcagcc gagcgttcaa gaaggcaacc ggtctcgccc
      841 ccggcgcctg gcgggaggcg cgtcgccctg cccgattgac caatccctga ctcgcacgcg
      901 tacgcaggag caggaagtcg ggacgcccag tcatttgccg aaggaccgtc cctggtgaga
      961 atgaggtccg cacgccgcga cggcgcgccc tgacccattc accgagaggt tgccatgtcc
     1021 accaagacca ttcccgccgt ccgccggccc ggcgatcacg gcgcggcgcc gattagccgg
     1081 cgtgccgagg ccctcgccgt ccggctcgag cagggggcca gcgcactggc cagtctcgcg
     1141 agcaccctga cggaggcgga gtggcagacg aggatccccc gagacgggcg caaggtcggc
     1201 gtcgtcgtgc atcacgtcgg gaacatgtac ccgatcgaga tcgaggcggc gctgaccctg
     1261 gccgcgggca atcctgtcgt gggcgtgacg gcggacgtca tcaacgagat caatgcgcgg
     1321 catgcccagg agttcgacgc cgtcacgaag gacgaggcgc tggagttcct ccggcggaac
     1381 agtgcgaccg cggcggcggc gatccgggcg ttgagcgacg aacaactcga tcgggccgcg
     1441 acaatatcgc tcaatgccga tgccccgctc acctgtcagt tctggctcga ggatcacccc
     1501 gtccggcaca gctatcacca tctcgccaag atccgggcgg cattggggcg atagttcgag
     1561 gcgggccgcc ggcgcggaac atcgtggttc tccgaccggg ggcgcgttag agggaggcgg
     1621 gagcctgtgg gtcgtgcgga gtcagggcgc ccgatagatc acgccgtttt tcatgacgaa
     1681 gcgcacccgg ctcaccaccg cgatgtcgcg cgtgggatcc ccggccaccg ccaccaggtc
     1741 ggccttcaac cccggggcca cgcgcccgac ttcgtgctcc atgccgagaa tcctggcatc
     1801 caccgcggtg gcggcgagca gggcctgggc cggccgcatg ccgtcgcgca ccatccactc
     1861 gagctcgcgc gcgttggcgc cgtgggtgaa cacgccgacg tcgctgccgc agccgatcgt
     1921 caccccgttc ttcaacgcga gctgaaaggc ctgggcggcc tgccgcatac cggcggtagg
     1981 gtcgcgcccg cgcacgtatc cctcgaagta ctcggcgtac gcctccaccg ctgtcaacgt
     2041 gggaagccag gccacgccgc gttccgccat caggtgaaac agcgagtcgg tgccgccgaa
     2101 cccgtgctcg atggtgttga cgcccgcgag gatggcccgc cgcatcccct cgggcgtgct
     2161 ggcgtgcgcg gccaccggcc gcccggaggc gtgggcttcc gccaccaggg catcgagctc
     2221 ctcctgactg aacgtgggaa cggcggcgcc gcccggtccc cggccgtaat ccgcgtacac
     2281 cttgatccag tcagcgcctc ggccggcctg ctcgcgcacg gcctcgagga cctgcggcac
     2341 gccgctcacc gcctggccgc cctgcggtag atcgaggtcc ggccggaagc cgcgcgggcc
     2401 ggggccgtag ctcccggtcg cgacgatggc gcgcgtggcc acctgcaacc gcggcccggg
     2461 gatgagcccc tcgacgatgg cccgccggag cgacacgtcc tcgaatccgg cgccttcggt
     2521 gcccaggtcc cgcagcgtcg taaacccctg catcagcgta ttgcgggcat ggaggacggc
     2581 ctcgaccacg cgatagcctt cgggctcctt gagcacctga tcgttccaca gcgtctcgtt
     2641 gtacggatgc agaaagagat gggagtgcgc gtcgatcagc ccgggcagga gcgtgaggcc
     2701 gggcaacgcg atggtctccg tgccgcgcgg gatgcgcacc gcgtccgccg acccgaccgc
     2761 ggcgatgctg tccccctgca ccagtaccac ccagcccgta tgggccgctt cactggtggc
     2821 atcgaacacg cggtccgggc gcagcagcac cgggtgtccg ggcgcggctg gcacgagcgg
     2881 ggcgaacccg gcgcgcagcg gttcctcgag cgccgagagc agcgtgtccc gtgcctgcgc
     2941 gccgcctcgt gtgggggcga tggcgaccag gagtacggca atcgtggaaa ccccgctgtt
     3001 cggacgcatg gatgaagctc cttgtccctg gcgtgatgct ctcagttctt gaggatcgcg
     3061 agcgcgcgct ccagcacttc gtcacggccg gcccggatcc ccgccagggt cggacggacc
     3121 acgacatccg gctgcaggcc gatccgctgc agctgccgcc cgtcagcatg gcgcacgtcg
     3181 tgacccgtga acgtggcgta catgccggcg acgagcgcca cggtcgtgac gtccccgttc
     3241 gcccccatcg tcggcgagcc cacgatcgtc gtcctgttcg cggcttccag gaacagcccg
     3301 gtgtgctccg cttggctgat ggtgcgctcg tccaccagca gaatggtctt cccgggatac
     3361 acccacttcg acccaggcgg cgtgctctga atgaagttgt acgtggtcca cgccgcggaa
     3421 tcgggactca tcaccagggg gcgctggaac gccgccgccg gcaccggggc ggcgctgagc
     3481 cgcggggcga tgctccacgc agttccctgc ggatatccac gaccgtcgaa gatgatgcct
     3541 ttcgtgttct tgaacagctc gaacatgctg tccaccatcg ctggggtgag gagcgagagg
     3601 tcggcgtacc cgacgttccc cgtgagcacc tgcaacacgg gtccgcttcg cggatattgc
     3661 atgagccggt cgaagctctg gcgccgtggg agcaccagcg cgcggtcgcg gttgtcgcgc
     3721 ccgcgcacga taagagttgc gacggagtcg gtgccacgca gcagccgctg cgcgatggcg
     3781 ttgtcgagcg cctgcggcgt cgaagacgcg acgaacctcg ctatcctcgc ccgccgcgcg
     3841 accacgtcct cgccgtccac cttgacgacc acgtcgcccg cggcgatgcc gctcgctttc
     3901 gtggccgagt cgtcaccgac acggatcacc accggttgac ccccgatgta ctggagctgc
     3961 accgcagccg gtagcacccc aaagtacgcg ctgagcagcg tgttgcggat caccccgctg
     4021 tgggaatcgt gcgtgtaggt caccatggtg gcgagcgcca tacccagctc gagcgagtcg
     4081 cgcgccgcct cgagcatccg gatgctgcgg ggcaggacgt cggcccagtc ctcgccgatc
     4141 aggtgcttgt agggatagaa gtagtggatg gtgttccagt agcgataggc ggcgaggatg
     4201 cggtacgctg cggacgggga gcgcatcccg gggtacgcgt cgtccgcggc cggcacgtgt
     4261 gccgatggca cccgggggaa gaggggggcc gtcaccggcc gtttcgcgaa ggcgatggcg
     4321 acgtccaagg ccgcgtcgcc tgcgccgacg gctccgtgag atacgaccgt gtcggcacca
     4381 gccggaccga cgacctcgct caggcggacg gtcgccgtga cgccctcacc catttgcacg
     4441 tcgtacgtct cggaacctgc gcgcagctgg gcgtccgccc cctcggcgac gatcgcgccc
     4501 tgtcccgcgt tgcgcagggc gaacgcgact tcggggatat ccgtgcccgg ggtgacgagg
     4561 aagacgatgc gccgcagacg gttgttcgag gaacctcgaa tcaccccgcc gtcccgtgtg
     4621 tacgagcccg accaataccc gcctgacgtg ccacccagct gcggcacgaa cccggaatgc
     4681 atgcgcaggc gctgtgcagg cgcgctgatc gccgtcgtgg cgagggcacc ttgaatggtg
     4741 ttggcgaaga cgaatgagga gttgcccaat tcctccggct cgggactccg aaggtcgaag
     4801 accagacccg gtgcgcgtcg aacgtcggcg gccgcgctgt tcaggagctg catcgcagcc
     4861 acgtaattgt cgaagtccgg tatcgcgatg accagcgtgc tgtccgttcc cgagaccatc
     4921 cagcgttgtg acggcgacgt ggtgcccgca ctcctcacct ccgaggcatt cccggcgcta
     4981 atccgagtcg cgggatcgcc gaggaccccg agcaggttgg ccaccgcggt gcgatagtcg
     5041 tcggcgctcc gggccgcacc caccgcgtcg atggccacga caacggcgga atcccagggg
     5101 acgtcgtgat cgagaaacgc gggatggaaa tacttgacca cgccgaacag cttgcccagc
     5161 gcggtaagtc gcgcgatccg gagcgagtcg ctctgcgcgc gcaggggcgt ggagatgagc
     5221 ccgccgagga atgcgacggc gagaagacgt cgagacaggt gcatgacgac ctcggtatcc
     5281 ctggaaatgg cctctgcccc tactccattc gtgtagtcca acgaccgaca cgccgcttcc
     5341 gaagtccatc acacggcatc gaggccgtgt ctgattctgc cggccccgcc cgaccgctag
     5401 ccttgcctgc gtcaacgatg gttaccactc acgacggaga acacgatgaa acccacaatc
     5461 tatgccccaa tcctgggcct ggtggctttg gcggcgtgca atagtctcaa ttccacgggc
     5521 tacaccgctc ccccaccgcc cgcctacacc acctacgaaa cgctgggcga cagcgtctcg
     5581 atcgcgacca agctggacga gtttcgggcc gcgctcggcg gcgccctcaa cgcgcccaac
     5641 gcgcctcccg ccgcgagcgg ccgacgcgag atcaactggg acggcgtcgc cgccgcgttg
     5701 accaacgtcg acaacttccc ggcggacttc ttcaacgtca actcgaagcg aggcgccgtg
     5761 ttcgcgactc ccggaaccgg tctccggatc gacagcacca acttcgccgc gatcaacgcc
     5821 ggcctgggcg cccagttcaa gttcttctcg cccaagaaga cgttcatggc ggtgggcagc
     5881 aaccaggtcg acgtcgattt caagctggtc ggcaccacga ccaacggact ggtcaacggc
     5941 ttcggcgtcg tcttctccga cgtggaccgg ctcggctcga cgacggtcga actgttcgac
     6001 gccaacgacg tccttatcgc cgtgctccgg gcccccaatc acagcggcgc gcagctgctg
     6061 tcgttcaccg gcgccgtgtt cgcttcgccg atcatcgccc gggcgcggat caccagcggc
     6121 gacgctgccc tgaacgggac gatcctcgat ctgagcgcgg gcggcaccgc ggatctcgtc
     6181 gtcatggacg atttcgtcta cggcgagccg cagccgctac cgcagtgatc cgatccactc
     6241 actcatcttg cacggagact gttatgcgat atacgacatg gatgacggca gccgccctcc
     6301 tcgccctggc ggcgtgcggc ggcgtgactt cacccggtgg gggtggcggg ggcggcggcc
     6361 ctgtcggcga catcacggtg gggaacatct tcttccggag tgtccacaac ggaacccaga
     6421 accccgccgt ggacacgatc gcggccggca gcagcatcac gtgggcctgg aacgccgcgg
     6481 gatcccacag cattcgctcg accggcacgc cggggatctt caggaacagc gtggtgatgt
     6541 ccggagctag cgacacatac accgtcacgt tcaacacgcc gggcacgtac acgtacgatt
     6601 gcgcggttca cggctcggcc atgaccggcc ggatcgtggt ccagtaggaa tgcagggacc
     6661 gttgattcac gcaccactct ggggtcccgg cgcgtccggg gccccaccct ccttccgtgc
     6721 tgggggcatc ctccccattt tccgggccag gagacctcat gccgtcagca ctgccgcaac
     6781 caccggagtt caatactccc gcgctcctgg aggcgttctt cgagcgctcc caggacgggt
     6841 tcttcttcat gatgctcgat gaaccgatcg agtgggggcc gggggtcgat aaggacgcgg
     6901 cgctggacta cgtcttttcc catcagcgca tgaccaaggt caatccagcg atggcgcagc
     6961 aattccgggc caggccggcg gagctgatcg gtctgacacc gggtgagttc ttccggcacg
     7021 acctcgcggc cggccgccgc ggctggcggg cactgttcga cgccggtcac acgcacagca
     7081 tcaccaacga gcggcgactc gatggaacga cgatgtgggt cgaaggcgac tacatgtgtt
     7141 tctacgatgc gaccggtcgg atcacggggc acttcggaat tcagcgggac gtcaccgacc
     7201 gcaccctcgc tgcaaaggaa ttggagcagt ctcgggccga gttgcgggcc ctcgcggccc
     7261 ggctccagac gacgcgtgag gaggagcgga cccgcatcgc gcgggagatt catgatgaat
     7321 tgggccaggc gctcaccgcc ttgaagctcg acctggcctg gctcgggacc aagcgacccc
     7381 ggggcaattc cggagccctc cggctgaccg acaagtcgat caccacccgg atcgacgaga
     7441 cgatggaggt cgtgcgccgg atcgccagcg agcttcggcc cagcgtgctc gaccagctgg
     7501 gcctggacgc cgccatcgag tacctggtgc aggaaacgac caagcggacc ggcatagcgg
     7561 tcaccctcca cgccgaggag ttccccaagc tccccgacga tgtggcgagc cacgccttcc
     7621 ggatcattca ggaggcgctg accaacgtcg cccgacattc caaggccacc cgggtggacg
     7681 tgaccgtgcg ccgcctggag gcggccatca tcctcggggt cgaggacaac ggcgtcgggt
     7741 tcaagccgca atcgctgagc gggcttcggt ccctgggatt ggtgggcatg cgggagcggg
     7801 cgttggcctg cgggggcgcc ttgacgattc gcggcaaccc gggcgagggc accgcaatcg
     7861 tggtcacgat tccggtcgcc gcagcgcgag ctgactgacc gtgattcgca tcctcatcgc
     7921 ggacgaccat ccgatcgtcc ggcagggact gcgtcgcatc gtggaggcgg acgcgggact
     7981 ggtgatttca ggagaggtgg gcgacgccgc ggcactgctg agcgcggtcc agaccacacc
     8041 caccgatctg gtgctgctcg acgtgtcgat gccgggtggc tcgtttctcg agacgctgca
     8101 gaacctgcgt gcccgccacc cggccatccg ggtcctggtc ttgagcgttc accccgaaga
     8161 ccagtgggcg gtgcgggcgc tcagggccgg ggcctcgggc tatctcacca aagaccactc
     8221 gccggatcag ctgctcgagg ccatccgccg ggtctaccgt ggcggcaaat acgtcagtcc
     8281 ggcgctggcg gaacagctgg cctcgcaatt cggtggcggc gagcagcaat ccctccatga
     8341 actgctgtcc gatcgcgagt tcgaagtcat gcgccggctg ggcagcggcc tcaccgtctc
     8401 ccagatcgcc ggcgaactga agctctcagc caagacggtg agcacttacc gtaagaggat
     8461 tctcgataag atggcggtca ccaccaatgc cgacctggtc agctatgccg ccaggcatgg
     8521 gctgatcggt tgacgcgccc ggcgcaggcg ctctgcttcg tgttcaggca tcgctcgagc
     8581 ggccggtgac gcggctcggg cgccggaaat acgccgaggc ctccgggcga agcagcagat
     8641 acgcgacccc caccagcagc acggcatgga cggcggcctc ggaccaggag tgcaacgcgc
     8701 tcagcacgac gtggtacgcc atccatgcca ccagaagcca ggacgcccag cgggccccac
     8761 gcaggacgaa cgctcccccg acgatcgcgg ccagccggac caggagaacc cagatcactt
     8821 cggagtcgaa cggacccggg gcgcgcagct cggtcgcgtg gtagacgagc ccgacgctcc
     8881 ccgcggcgac gaacagccag ccgacgacgg tgacggagag tgggcgcccg gtaggcgagg
     8941 gggcgtcgtc cggagcggtc acgatggagt cattgccgtc gggatcttca atgtggagct
     9001 cgtcgtatcc ccaccaggag tccttgctgg gacgagggag ccgatgctgg cccatcgatg
     9061 ccgtgccacc tgacgcattg cactgtagct gaagggtgcg gcagccgcaa tcaagaggga
     9121 ccccttccct ggactgtgac ctcgcccgcg agtcgtcagc acacgggtgt ctcccgtgag
     9181 acagccaccg ggcgaacctg ccgctgatgg cgattgtcga ccgaggctgg gtcagcttca
     9241 acgcgggcgc ttgacgctcg cttgatcgag gtaccgcatc atacctcatc gcaatgcacc
     9301 gcagggcgcc aattgcagcc acattaggcc aggaggacgg atgcgttcca taacactcgt
     9361 ggggctcgtt gggctcaccg ccgcgtgcag cggtgccgcg gtccaagaac cgcctgctgc
     9421 ggtggcgatc accatcgcgg cagtgggaac gctccgctac ggggacggag ctccagtcgt
     9481 gggcgcaaca atctacaagt atgccacatt gcttagcccc agcgaacaga gcacactctc
     9541 agatgccaac ggcaacttcg cgctcaccta tgtcgataga tgtattccag gcagctactc
     9601 cagcggagtg ggtatcacgc cgcccgcgac ccgaccgatt ggcgcagccg tatgcaccgg
     9661 cgagatcatg tgtatcaccg gaacccagcg cagggactgc accttctagc gcaggggtga
     9721 agggtccccg ggggtgtcag gccgccgcag gattgggcga gattccccgc cggcggatcc
     9781 tgaagccgag ccaagcggcg aggagagccg ggagaaggac aacgacagtt tcttgagcca
     9841 attgggccgc gagactcgcg tgcagaccgc ccagcggggt ccagggaaag cggaagcgat
     9901 ggtgggagaa cggtgcgagg aactcgacac ccagggagta cgtcgagagc gcgtcgagac
     9961 aggaatggga gatggatgca acggctagcg tgatgccaat ccgcgtgcgg tactgcatcc
    10021 agaggcctct tcggaagaag aaggcggtgg tgagtagcgc actgacgagt gcgaaagtga
    10081 gcgaatgcgt aatggcgcga tgggcgatgg tcgaggtatc agcgatgtga aggacccatc
    10141 cgatcgcatc gatgtctggc agcgccgcgc acgctgcggc tgcgaggcaa actcgagcgg
    10201 agggccttcc gggctggatc caggccccga gggcatagcc gacggcggca tgggagagag
    10261 gtgtgggcac ggtgtcctgc agcggcctaa cggattgcgc ttacgctgcg gggtggggcg
    10321 gcggtaatca agatagatcc cttccgtacc aagcggccgc ggcgccccag aagcactcat
    10381 tgggaagcca attgccgcac cgattactgc gatggcgacg gcgacttacc gcttgccgaa
    10441 tcgtagagaa actcccgcgc tcaagagcca agcctctgac ttcacatcct ggggtgagcg
    10501 ttgaacgacg agcttggtga gacccagatc gtggcgcgcg aggagcgtga aggggccgac
    10561 gttgaatcct ccaccgaaga tcaggagagc atctgtgtgc ctcatcgagg cgccttctgt
    10621 tgcatacagg ctgtcgcagc tcgccacggc aataggaccg gacgaactga aggtgcacga
    10681 agtctgaaac gcgagggccg gtccggcgaa gacggtaggg gcgaagcgga gccgaccggc
    10741 gattgggtag gtgcccgtga agagcagcgg gagctcaacg tagtcctgcc tgtagtccac
    10801 cgtggatggg caggacgccc cggggccgca gggcggcacg atgagctcgg ttcccttccg
    10861 gacgtacagg atctggggtt cgacggcgaa gtacttgtag atgct
//
LOCUS       DBNGZW010000014         4684 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1160799, whole genome shotgun sequence.
ACCESSION   DBNGZW010000014 DBNGZW010000000
VERSION     DBNGZW010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1160799"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="AC16JC_00370"
     CDS             <1..355
                     /locus_tag="AC16JC_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163177.1"
                     /translation="ATGLPGRPGTAARTSSMSPGDREVVMPKAVKWKPRLLKAARDAK
                     QRAMAAVKHADALMRAARQKAETEARRRKVRQAMRTAGRALKAAGKAALAAGAAAALA
                     AIVSERSQTKPKRRT"
     gene            complement(362..574)
                     /locus_tag="AC16JC_00375"
     CDS             complement(362..574)
                     /locus_tag="AC16JC_00375"
                     /inference="COORDINATES: protein motif:HMM:NF016234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CstA-like transporter-associated
                     (seleno)protein"
                     /protein_id="HIR5163178.1"
                     /translation="MPSRDGGRGTREGALPLPTRIARVIRRIIGAPDYGAYLEHCRLA
                     GHPPHLDERAYLNEFFASKGKGVRCC"
     gene            complement(564..2591)
                     /locus_tag="AC16JC_00380"
     CDS             complement(564..2591)
                     /locus_tag="AC16JC_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000854671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HIR5163179.1"
                     /translation="MPRLFRALIWVALSLLGAAAFAILALSRGETVSAAWVIVAAICT
                     YVVAYRFYARFIATRVFGLDARRATPAERLSNGRDFVPTNKWVLFGHHFAAIAGAGPL
                     VGPVLAAQFGFLPGTLWLVIGVVLGGAVQDFTILCCSVRRNGKSLGQMAREEINPLAG
                     STAMLAVLFIMIILLAVLALIVVNALKASPWGLFTIACTIPIALLMGWWMKSWRPGRI
                     GEASLVGAVLLIAALVGGGWVASHPIGTAFTQSGTALTWMMMGYGFAASVLPVWMLLA
                     PRDYLSTFLKIATIVVLAVAIMVILPPLRMPALTAFATLGEGPVFAGKFFPFVFITIA
                     CGAISGFHALVSSGTTPKMLSRETDARLIGYGGMLMESFVGVMAMIAACTLEPGVYFA
                     MNTTPAALAGAADVLRQAGFVVTPDQMQALAAQVGEQTLIARTGGAPSLAVGIAHIFS
                     GVSSALGGRAATALWYHFAIMFEALFILTTIDAGTRVGRFMLQEIVGHVWPRFGETSW
                     YPSIVASSAVVVAGWGYFLYQGVVDPLGGINSLWPLFGISNQLLAAVALCVGTTILIK
                     MGRARYSWVTLAPLAWIVTITMTAGWQKVFSADPKLGFLSHAASLAASTDPRASRLIF
                     NDHLNTFVALLFMSVVALLVVVSIREWWLVLSKRKPAAVSEAPYVESRYAV"
     gene            2649..3512
                     /locus_tag="AC16JC_00385"
     CDS             2649..3512
                     /locus_tag="AC16JC_00385"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5163180.1"
                     /translation="MSSSSSWSMAARQAPSPTGEAVTQERPDPQVAAERLGGRCALVT
                     GGATGLGRAICLELAQRGTAIGFNYVNLPGRDVSAQALLTETAIKAHGVAVYSEHCDV
                     RRPEEVEHFVTEARERLGGVHILINNAGVTHDGALWRLSTDSWQEVLDTNVTGAFNCI
                     RSVAGHMRAQRYGKIVNISSHQAFRPGFGIANYAASKAALIGLTKAAAVDLGSSNVNV
                     NAVAPGFVKTELLAQLPREILERAERESVLGRVAEPEDVARVVAFLCSDAARHITGQT
                     IVVDGGLTLAS"
     gene            3592..4215
                     /locus_tag="AC16JC_00390"
     CDS             3592..4215
                     /locus_tag="AC16JC_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163181.1"
                     /translation="MAVLYAATACGGKPAANQQPTPEPTRPLPLAGLASVKVPVLPLT
                     LLAFDDSLPWASGFADRRAALGIADSILGTLLQGRAPEVTWILPDELRRQARRSPTFA
                     ANPDQMGTAILRGAKVGDFLPDPLHSELRALVAIADARHALVPAQLVYRRTAGSVDGR
                     MGTAELTVVIVDARLGQVGFKTVARGDGTDPWTALTRAVKALTPGLP"
     gene            4263..>4684
                     /locus_tag="AC16JC_00395"
     CDS             4263..>4684
                     /locus_tag="AC16JC_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011464696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR5163182.1"
                     /translation="MQATLLVLDALGVKFDWDEEYGGMSAVDKAGTPLPNATLDSIRH
                     TKLALKGPLTTPLGGGYRSVNVALRQEFDLYANVRPTKTLVPGGRFEGIDLVIIRENT
                     EGMYVGLDHSFRVGDDPRAVAQATSVMTREGCRRICRFA"
BASE COUNT          787 a         1592 c         1681 g          624 t
ORIGIN      
        1 tgccaccggc ctccccgggc gtccggggac ggccgcacgg acgtcatcca tgtcacccgg
       61 ggatcgggag gtcgtcatgc cgaaagccgt gaaatggaag ccgcgcctgc tcaaggcggc
      121 gcgcgacgcc aagcagcgcg ccatggccgc ggtgaagcac gcggacgccc tgatgcgggc
      181 ggcccgtcag aaggccgaga ccgaagcccg ccgccgcaag gtgcggcagg cgatgcggac
      241 ggccggccgg gcgctgaagg cggccgggaa ggcggcgttg gccgccggag cggccgcggc
      301 gctggcggcg atcgtgtcgg agcgttccca gaccaagccg aagcgccgca cgtagccgcg
      361 gctagcagca gcggacgccc ttgcccttgg acgcgaagaa ctcgttcagg taggcacgct
      421 cgtcgaggtg cggcggatgc ccggccagcc ggcagtgctc gaggtaggcc ccgtagtccg
      481 gcgccccgat gatgcggcgt attacccgtg cgatccgggt ggggaggggt agggcccctt
      541 ccctcgtccc tcgtcccccg tccctagacg gcataccggc tctccacgta cggcgcttcg
      601 ctgaccgccg ccggcttccg cttcgacagc accagccacc attcgcggat cgacacgacg
      661 accagcagcg ccacgacgct catgaacagc agggccacga acgtgttcag gtggtcgttg
      721 aagatcaggc gggaggcccg cgggtcggtc gaggcggcga gcgacgcggc gtgcgagagg
      781 aagcccagct tggggtcggc tgagaacacc ttctgccacc ccgcggtcat ggtgatcgtg
      841 acgatccagg cgaggggggc gagcgtgacc caggagtagc gagcgcgtcc cattttgatg
      901 aggatcgtcg tccccacgca cagggcgacg gcggcgagca gctgattcga gatcccgaac
      961 agcggccaca gcgagttgat cccgcccagc gggtccacga cgccctgata gaggaagtac
     1021 ccccacccgg caaccaccac ggcgctcgac gccacgatgc tgggatacca cgacgtctcg
     1081 ccgaagcgcg gccagacgtg gcccacgatc tcctggagca tgaagcggcc gacacgcgtg
     1141 cccgcgtcga tcgtcgtgag gatgaacagc gcctcgaaca tgatggcgaa gtggtaccac
     1201 agcgcggtgg cggcccggcc ccccagcgcg ctcgagaccc ccgagaagat gtgcgcgatg
     1261 ccgaccgcga ggctgggggc gcctccggtg cgggcgatga gggtctgctc gcccacctgg
     1321 gcggccagcg cctgcatctg gtcgggggtg acgacgaacc ccgcttgccg caggacgtcg
     1381 gccgccccgg cgagcgctgc gggggtcgtg ttcatggcga agtacacgcc cggctcgagc
     1441 gtgcaggccg cgatcatcgc catgacgccg acgaacgatt ccatcagcat cccgccgtag
     1501 ccgatcagcc gggcatcggt ctcgcggctc agcatcttgg gagtggtgcc cgacgacacc
     1561 agagcgtgga accccgaaat ggcgccgcag gcgatggtga tgaacacgaa cgggaagaac
     1621 ttgccggcga ataccggccc ttcgcccagg gtcgcgaacg ccgtgagcgc cggcatgcgc
     1681 agcggcggca ggatgaccat gatcgccacc gcgaggacga cgatcgtcgc gatcttgagg
     1741 aacgtggaga ggtagtcccg cggcgccagc agcatccaca ccggcagcac cgacgcggcg
     1801 aatccgtaac ccatcatcat ccacgtgagc gccgttccac tctgcgtgaa cgcggtcccg
     1861 atcggatggc tcgccaccca cccgccgccc acgagcgcgg cgatgagcag cacggcgccc
     1921 acgagcgagg cttcgccgat gcgccccggc cgccagctct tcatccacca gcccatgagc
     1981 agcgcgatgg gaatggtgca ggcgatggtg aacaatcccc acgggctcgc cttgagagcg
     2041 ttgacgacga tcagggccag cacggcgagc aggatgatca tgatgaacag caccgcgagc
     2101 atcgccgtcg agcccgccag cggattgatc tcctcccggg ccatctggcc gagcgacttg
     2161 ccgttgcggc gcaccgagca gcagagaatg gtgaaatcct gcaccgcacc gcccagcacc
     2221 acgccgatca cgagccacag cgtgccgggc agaaacccga actgcgcggc gagcacgggg
     2281 ccgacgagcg ggccggcgcc ggcgatggcg gcgaagtgat gcccgaacag cacccacttg
     2341 ttggtgggca cgaagtcccg tccgttggac agccgctcgg cgggtgtggc gcggcgtgcg
     2401 tcgaggccga agacacgggt ggcgatgaac cgggcgtaga accggtaggc gacgacgtag
     2461 gtgcagatcg cggcgacgat cacccaggcg gcgctgaccg tctcaccgcg cgacagggcg
     2521 aggatcgcga aggcggcggc gcccagcagc gacagggcca cccagatcag tgcgcggaac
     2581 aggcgcggca tggccccaag ttacggcgcg gcttgcaggg cttcaaatcg ccccgtaggt
     2641 tcgacagctt gtcttcctcc tcgtcctggt ccatggccgc gcggcaggcg ccgtccccga
     2701 cgggggaggc ggtgacccag gaacgccccg atcctcaggt cgccgcggag cgcctcggcg
     2761 ggcgctgcgc gctcgtgacg ggcggcgcca ccgggctggg ccgcgccatc tgcctcgagc
     2821 tggcgcagcg cggcaccgcc atcgggttca actacgtcaa cctccccggc cgcgacgtct
     2881 ccgcacaggc gctgctgacc gaaacggcga tcaaggcgca cggcgtcgcc gtgtactccg
     2941 agcactgcga tgtgcgtcgc cccgaagaag tggagcactt cgtgaccgag gcgcgcgagc
     3001 ggctcggcgg ggtgcatatc ctcatcaaca acgccggcgt cacccacgat ggagcgctgt
     3061 ggcggctctc gacggattcg tggcaggagg tactcgacac caacgtcacc ggcgccttca
     3121 actgcatccg ttccgtggcg ggacacatgc gcgcccagcg ctacggcaag atcgtgaaca
     3181 tctcgagcca ccaggccttc cggccggggt tcgggatcgc caactacgcc gcgagcaagg
     3241 cggcgctgat cggcctgacc aaggccgccg cggtggacct cggctcgtcc aacgtgaacg
     3301 tgaacgccgt cgcgcccggc ttcgtgaaaa cggagctgct ggcgcagctg ccgcgggaga
     3361 ttctcgagcg cgccgagcgg gaatcggtgc tgggacgcgt ggccgagccc gaggacgtcg
     3421 cgcgcgtggt cgcgttcctg tgcagcgatg cggcgcgcca catcaccggc cagacgatcg
     3481 tcgtggacgg cggactcacc ctggcttctt gaaaacgcgg cgccgcggct ggatattgcg
     3541 tgcgtgacta atccattgaa aaacagaagc ttagtgcgtg gggagtgggt cgtggcggtg
     3601 ctgtacgccg cgacggcctg cggcggcaag cccgccgcaa atcagcagcc gacccccgag
     3661 ccgacgcgtc cgttgccgct cgcggggctg gcgagcgtga aggtccccgt gctgccgctc
     3721 accctgctcg cgttcgatga ttcattgccg tgggcgtcgg gcttcgccga ccggcgcgcg
     3781 gcgctcggga tcgccgacag catcctcggc acgctgctcc agggtcgagc gcccgaggtg
     3841 acgtggatcc tgcccgacga gctgcggcgc caggcgcgcc gctcgcccac gttcgctgcg
     3901 aaccccgacc agatggggac ggcgatcctg cggggcgcca aggtaggaga tttcctgccc
     3961 gatccgctgc acagcgagct ccgggcgctg gtggcgatcg ccgacgcgcg ccacgcgctc
     4021 gtgcccgcac agctggtcta ccggcggacg gccggttcgg tggatggccg gatggggaca
     4081 gccgagctga cagtggtcat cgtcgacgca cggctgggac aggtcgggtt caagaccgtg
     4141 gcgcgcggcg atgggaccga tccctggacg gcgctcacgc gggcggtgaa ggccctgacg
     4201 cccgggctgc cgtgacgcgc gaggccaccc tcatcgccgg cgacggcatc ggtccggaga
     4261 tcatgcaggc gacgttgctc gtgctcgatg cgctgggcgt gaagttcgat tgggacgagg
     4321 agtacggcgg gatgagcgcg gtggacaagg cggggacgcc gttgccgaac gccacgctcg
     4381 actcgatccg gcataccaag ctcgcgctca agggcccgct cacgacgccg ctggggggcg
     4441 ggtaccgttc ggtgaacgtc gcgctgcgcc aggagttcga tctctacgcc aacgtgcggc
     4501 cgacgaagac actcgtcccg ggcgggcgct tcgaggggat cgacctcgtg atcatccggg
     4561 agaacaccga agggatgtac gtggggctcg accacagctt ccgggtgggc gacgatcccc
     4621 gggcggtggc gcaggcgacg tcggtgatga cgcgcgaggg atgccggcgc atctgccgct
     4681 tcgc
//
LOCUS       DBNGZW010000015         3234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_547057, whole genome shotgun sequence.
ACCESSION   DBNGZW010000015 DBNGZW010000000
VERSION     DBNGZW010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3234
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_547057"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="AC16JC_00400"
     CDS             <1..658
                     /locus_tag="AC16JC_00400"
                     /inference="COORDINATES: protein motif:HMM:NF024839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR5163183.1"
                     /translation="RHLASVALARQPETCGQCHMGPDHSQIEIYDESKHGVLYRLQWP
                     TMRLDAAPERLTTTDMPVPTCATCHLSGLNGGATTHDVGQRLSWWLFADVSEHRPDWQ
                     RSRNAMAAVCLNCHTRPTVDSVYAQADAVVAATNTKVREAEALVAGLRQRQLLTSQPF
                     DEPIEFAVFDLWHYYGRTAKHGAFMGGPDFVQWHGNYELLLKTAELRDMAAALEHGRR
                     "
     gene            645..1490
                     /locus_tag="AC16JC_00405"
     CDS             645..1490
                     /locus_tag="AC16JC_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163184.1"
                     /translation="MVGARPRRPLALGFLVELFAIANLAFLAVDIFLAHTVNRFRAWP
                     EWIPIGFSSAAAPALALGLWWANRHGDEWRPARGGVAVGVLAIVVGIAGLLFHLESQF
                     FQSMSLKSLVYTAPLAAPLAYTGLGLLLLLNRMVRPESLEWDRWVALLAGAGFVGNFA
                     LALLDHAQNGFYYVSEWIPVAAGAYAVAFLGMVVLRPHDRALLLVTLATLAAQGVVGV
                     LGFAFHLYADLTGPGGGLRDDFLYGAPVFAPLLFTNLALLASLAVWDLWRTLPMDNPE
                     IPGDA"
     gene            1487..2872
                     /locus_tag="AC16JC_00410"
     CDS             1487..2872
                     /locus_tag="AC16JC_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5163185.1"
                     /translation="MTPPRPGTPSDPLRVAIVGAGPAGFFLAERLLGKEGITAEVDLY
                     ERLPTPYGLVRFGVAPDHEKIKNVTRSFDKIAARPGFRFFGNVDVGRDVTLDDLRRCY
                     HQVCFTTGAQTDRRMDIQGEDLARSHAATEFVAWYNGHPDYRDLTFDLSVERVAVVGV
                     GNVAVDVARMLCRTTEELAATDVADYALEALSCSRVREVYLLGRRGPLQAAFTNPEAK
                     ELGELEGADLRLLPEEVALDPLSQTELERGDDNAARKKIEILRDFSGRPPTGKPRLLT
                     VRFLVSPVELLGDDQGRVRAMRLVRNRLVEGKGGALRAEATDQFEELPADLVFRSVGY
                     RGVALPGLPFDERSGIVPNERGRVAPGVYVAGWIKRGPTGVIGTNKPDAAETAETMFA
                     DLERHALLSPGEPAAAAAEALVRRVRPAVVTYADWQRLDRYETERGKAVGRPRLKCTT
                     VGEMLEVLGRR"
     gene            complement(2894..>3234)
                     /locus_tag="AC16JC_00415"
     CDS             complement(2894..>3234)
                     /locus_tag="AC16JC_00415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015836971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163186.1"
                     /translation="GEFPPSWFARAKLCPDRHDPTLNFFGVNASQPLSVWRKKGWISA
                     QDPRGWFQWYCRYYMGRRCDDDARQIRRWRAVRRHVVQLRNACTPGDWSCRPRQRQAL
                     LHWAYDSRLI"
BASE COUNT          476 a         1107 c         1143 g          508 t
ORIGIN      
        1 ccggcacctc gcctctgtcg ccctcgcccg ccagcccgag acgtgcggcc agtgtcacat
       61 gggccccgac cactcccaga tcgagatcta cgacgaatcc aagcacggcg tgctgtaccg
      121 cctgcagtgg cccacgatgc gcctggatgc cgcacccgag cgtctcacga cgacggacat
      181 gccggtgccc acctgcgcca cctgtcacct gagcggcctg aacggggggg cgacgaccca
      241 tgacgtgggc cagcgactct cgtggtggct cttcgccgac gtgtccgagc atcggcccga
      301 ctggcagcgc tcccgcaacg cgatggccgc ggtgtgcctc aactgccaca cgcgcccgac
      361 ggtggacagc gtgtacgcac aggcggacgc cgtggtcgcc gccaccaaca cgaaggtccg
      421 ggaagccgaa gcgctcgtcg ccgggctgcg gcagcgtcag ctgctcacct cgcagccgtt
      481 cgacgagccg atcgagttcg cggtgttcga cctgtggcac tactacggcc gcacggcgaa
      541 acacggcgcg ttcatgggcg ggccggattt cgtccagtgg cacggcaact acgagctcct
      601 gctcaagacg gcggagctgc gggacatggc ggccgctctg gagcatggtc ggcgctaggc
      661 cgcggcgccc gctcgcgctc gggttcctcg tcgagctgtt cgccatcgcc aacctcgcct
      721 tcctggcggt ggacatcttc ctggcgcaca cggtcaaccg cttccgggcg tggcccgaat
      781 ggattcccat cgggttttcg agcgccgcgg cgccggcgct cgcgctggga ttgtggtggg
      841 cgaaccgtca cggcgacgaa tggcggccgg cacgcggcgg ggtcgcggtc ggcgtgctgg
      901 cgatcgtggt cggcatcgcc ggactgctgt ttcacctcga gagccagttc ttccagagca
      961 tgagcctcaa gagtctggtc tacacggcac ccctcgcggc gccgctggcc tataccgggc
     1021 tcggtctgct gctgctgctc aaccggatgg tgcggcccga gtcgctggag tgggaccgct
     1081 gggtggcgct gctggccggc gcgggcttcg tgggcaattt cgcgctggca ctgctcgatc
     1141 acgcgcagaa cggattctat tacgtgagcg agtggattcc cgtggcggcg ggcgcctatg
     1201 ccgtggcctt cctggggatg gtggtgctgc gcccgcacga ccgggcgctg ctgctcgtga
     1261 cgctggccac cctcgccgct cagggcgtgg tcggcgttct ggggttcgcc ttccatttgt
     1321 acgccgatct caccgggccc ggcggcggcc tcagagacga cttcctctat ggcgccccgg
     1381 tcttcgcccc gttgctgttc accaatctcg ccttgctcgc gagtctggcg gtgtgggatc
     1441 tgtggcggac gctcccgatg gacaacccgg agattccagg agacgcatga cccccccccg
     1501 tcccggaacc ccatccgacc cgctgcgcgt ggcgatcgtc ggcgccggcc ccgccggttt
     1561 ctttctcgcc gagcggctgc tcgggaagga ggggatcacc gccgaggtgg acctgtacga
     1621 gcgcctgccg acgccgtacg gtctggtacg attcggcgtc gcccccgacc acgaaaagat
     1681 caagaacgtc acccgctcgt tcgacaagat cgcggcgcga cccgggttcc gattcttcgg
     1741 caacgtggac gtggggcggg acgtcaccct cgacgacctg cggcgctgct accaccaggt
     1801 gtgcttcacc acgggcgctc agaccgatcg ccggatggac atccaggggg aggacctggc
     1861 gcgcagccac gccgcgacgg aattcgtggc gtggtacaac gggcatcccg attaccggga
     1921 cttgacgttc gacctgtccg tggaacgggt cgcggtggtc ggcgtcggca acgtcgccgt
     1981 ggacgtggcc cggatgctgt gccggaccac cgaggagctg gccgcgaccg acgtcgccga
     2041 ctacgcgctg gaagcgctga gctgctcgcg cgtgcgcgaa gtgtatctgc tcggccggcg
     2101 cgggccgctc caggccgcct tcaccaatcc cgaggccaaa gagctgggag agctcgaagg
     2161 ggcggacctc cgcctcctgc ccgaagaagt ggcgctcgat ccactgagcc agacggagct
     2221 ggagcgcggg gacgacaatg ccgccaggaa gaagatcgag atcctgcggg acttctccgg
     2281 acgcccaccg accggtaagc cgcgactgct gaccgtccgg tttctcgtgt caccggtcga
     2341 gctgctggga gacgaccagg gccgggtgcg ggcgatgcgg ctggtgcgga atcgcctggt
     2401 ggaggggaag ggcggcgcgt tgcgcgccga ggcgaccgac cagttcgagg aattgccggc
     2461 ggatctagtg ttccggtccg tgggctatcg cggcgtggcg ctgcccggat tgccgttcga
     2521 tgagcgatcc gggatcgtgc cgaacgaacg ggggcgcgtg gcgcccggcg tgtacgtggc
     2581 cggctggatc aagcgagggc ccaccggtgt gatcggcacc aacaagcccg acgccgccga
     2641 gaccgcggag acgatgttcg cggatctcga gcggcatgcg ctcctgtccc ccggcgagcc
     2701 ggcggccgcg gccgcggagg ccctggtgcg ccgcgttcgg cccgccgtgg tgacctacgc
     2761 ggactggcag cgcctcgacc gctacgaaac cgagcgcggc aaagcggtgg ggcggccgcg
     2821 gctcaagtgc acgaccgtcg gggagatgct cgaggtccta ggccggcgct gacgcgctcc
     2881 ggcctgcagg ggcctagatc agccgcgaat cgtaggccca gtggagcagc gcctgccgct
     2941 gtcgcgggcg acagctccaa tcgccgggcg tgcacgcgtt ccggagctgg accacgtgcc
     3001 ggcgtacggc gcgccagcga cggatctgcc gcgcgtcatc gtcgcaccgg cggcccatgt
     3061 agtagcgaca gtaccactgg aaccagccac gcggatcttg cgccgagatc cagcccttct
     3121 tgcgccacac cgacagcggt tgtgaggcgt tcacgccgaa gaaattcagc gtcgggtcgt
     3181 gacgatcggg acacagcttg gcccgggcga accagctcgg cgggaattcc ccgc
//
LOCUS       DBNGZW010000016         4654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_956323, whole genome shotgun sequence.
ACCESSION   DBNGZW010000016 DBNGZW010000000
VERSION     DBNGZW010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4654
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_956323"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1232)
                     /locus_tag="AC16JC_00420"
     CDS             complement(<1..1232)
                     /locus_tag="AC16JC_00420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR5163187.1"
                     /translation="MSRLRTLSAEYRELAQRLEQGGGPDKIRRQHDQGKLTARERLAG
                     LLDPGATWLEVGLLVASDRYDGQAPGAGVVTGVGRVHGRPVVVVANDATVKAGSWWPE
                     TITKILRAQEIAMRQRIPIVYLVDSAGVNLPYQGGVFPGQYGAARIFYYNSIMRRYLR
                     VPQIAAVMGPCIAGGAYLPALSDVIFMVEGTSFMGLGGPNLVKGATGQVVDAETLGGA
                     VTHTAVSAVAHYRAASDARCLEMIREYLERLPRNARPSPTGAAPPQGAAADLYDLLPH
                     DHRMSYDMRAVLDCLVDAGGFDEFQPDVGHEMLCGHARLGGRPVAIIANARGVIKGRK
                     GEPPRIGGIVYTESAEKAAYFIENADRERLPLVFVQDVSGFMVGTEAEQSGIIRAGAR
                     FVEAMATATVPKLVLTVN"
     gene            complement(1229..1660)
                     /locus_tag="AC16JC_00425"
     CDS             complement(1229..1660)
                     /locus_tag="AC16JC_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006694794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR5163188.1"
                     /translation="MSLAVDGRKRRPIRVLVAKPGLDGHDRGAKVVAAALRDAGMEVI
                     YTGLHQTPEMIAQAAIQEDVDVVGLSILSGAHMTLFPRVQSLLRQAGRTDVLLTGGGI
                     IPPEDMTALEGQGVGKLFGPGTPTGELVQYIEAWVADRLPR"
     gene            complement(1657..2769)
                     /locus_tag="AC16JC_00430"
     CDS             complement(1657..2769)
                     /locus_tag="AC16JC_00430"
                     /inference="COORDINATES: protein motif:HMM:NF037429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baeRF10 domain-containing protein"
                     /protein_id="HIR5163189.1"
                     /translation="MAALPFSPGKGTVNEVLDRLGRITPGGRQVVSCYLKLEPRDKAR
                     GKYLIKMKNRVKETAVGLAQRALNRSEREAVAADLDRVLRYLEEPGRLPQARGVALFA
                     CGALDLFEVIPLPQVQRSRLVVADAPATRELVALEQEFGTILVAACDRTGARFFTVTA
                     FGITERSGITSPASRTEKFHGERQVMRGAVPGSAGEHGHHGRIREEKHRHYARVAEEI
                     FRINVQTPLSGLVVAGIGVDAAALVPHLHTYLHDLLFGVVRLNPKTITPAEVREAALA
                     LREERERAWERAHAEAVKDGLGTGWAVNGIEATEKALARGQVRTLLVDGRDEDVRLDD
                     AVEEALAQRAQVDVLNDDRARRGVDGLAALLRFRRA"
     gene            complement(2769..4331)
                     /locus_tag="AC16JC_00435"
     CDS             complement(2769..4331)
                     /locus_tag="AC16JC_00435"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019851193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR5163190.1"
                     /translation="MSIERKPVYGARDLAGWDPAAQLGTPGAYPFTRGIHPTMYRGRL
                     WTMRQFAGFGTAADTNRRYKFLLARGQTGLSVAFDFPTLMGYDSDHPRAEGEVGKCGV
                     AISSLADMQTLFDGIPLDQVSTSMTINGPAAILFCFYVAAAERQGVPIAKLRGTVQND
                     ILKEYMAQHAWVYPVEPALKIIVDMFEWCAEHTPEWNTISISGYHIREAGATAAQELA
                     FTLANGFTYVERGVARGLNVDGFAPRLSFFWDIHNDFFEEIAKLRAARRIWARHMRER
                     YGAVSDRSLKLRFHSQTAGVTLTAQQPMNNIVRVAYQALAAVLGGTQSLHTNSLDETL
                     ALPTEQAVQVALRTQQILAHETGVPEVIDPLGGSYYLESLTDQLEREAEALFAEIQSI
                     GGVVSGIENGWFQRQIARSAAEFQRQVEEQRKVIVGVNDFLSEEDHPVEVLRITNDAE
                     DSQRRRMVELRAGRDQALVDRRLEALRQAAENDHNVIGPMLDCARVYCTLYEIRHALE
                     RVYGAYREPVFF"
     gene            complement(4328..>4654)
                     /locus_tag="AC16JC_00440"
     CDS             complement(4328..>4654)
                     /locus_tag="AC16JC_00440"
                     /inference="COORDINATES: protein motif:HMM:NF015279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163191.1"
                     /translation="PPPPPPTWEPPVLLTVATRGEGVTGLVEALERHRGYLEASGLLE
                     RRRRERLAERTRASVNRALAHWVWEQTAAETLLEEALDDMSAGKRSPYEVAAAIVDHA
                     RTGATR"
BASE COUNT          675 a         1702 c         1564 g          713 t
ORIGIN      
        1 tggttgacgg tgagcacgag cttggggacc gtcgcggtgg ccatcgcctc gacgaaccgg
       61 gcgccggcgc ggatgatgcc cgattgctcg gcctcggtgc cgaccatgaa ccccgacacg
      121 tcctggacga acaccagcgg cagccgctcg cggtcggcgt tctcgatgaa gtacgccgcc
      181 ttctccgcgc tctcggtgta gacgatgccg ccgatccgcg gcggctcacc cttcctgccc
      241 ttgatgacgc cgcgcgcgtt ggcgatgatc gcgaccggcc ggcccccgag gcgcgcgtgg
      301 ccgcacagca tctcgtgccc gacgtccggc tggaactcgt cgaaccctcc ggcatcgacg
      361 agacagtcga gcacggcgcg catgtcgtag ctcatgcgat gatcgtgcgg cagcaggtcg
      421 tagagatcgg ccgcggcgcc ttgggggggc gctgcacccg tcggcgacgg acgggcgttc
      481 cgcggcagcc gctcgaggta ttcgcggatc atctcgaggc agcgggcatc gctcgcggcg
      541 cggtagtgtg ccaccgcgct gacggcggtg tgggtgactg cgccgccgag cgtctcggcg
      601 tccaccacct gcccggtggc gcccttcacc aggttcggcc cgcccagtcc catgaacgac
      661 gtgccttcga ccatgaagat cacgtccgag agtgccggga ggtacgcacc gccggcgata
      721 cacggcccca tcacggcggc gatctgcggc acccgcaggt agcgccgcat gatggagttg
      781 taatagaaga tgcgggcggc gccgtactgg cccgggaaca cgccgccctg atagggcagg
      841 ttgacgcccg cggagtccac caggtagacg atggggatgc gctggcgcat cgcgatttcc
      901 tgcgcccgca ggatcttcgt gatcgtctcc ggccaccacg acccggcctt gacggtcgcg
      961 tcgttcgcaa cgacgacgac cggccggccg tgcacgcggc cgaccccggt gacgacgccc
     1021 gcccccggcg cctggccgtc gtagcggtcg gaggcgacga gcaggccgac ctcgagccac
     1081 gtggcgcccg gatcgagcag gcctgcgagc cgctcccgcg cggtgagctt cccctggtcg
     1141 tgctggcggc ggatcttgtc gggcccgcca ccctgctcca accgctgcgc gagctcacgg
     1201 tattccgccg acaacgtccg gagacggctc atcgcggcag gcgatccgcg acccatgcct
     1261 cgatgtattg gaccagctcg cccgtcggcg tccccggccc gaacagtttc cccaccccct
     1321 gcccttcgag cgccgtcatg tcctcaggag gaatgatgcc gccgccggtc agcagcacgt
     1381 cggtgcggcc ggcctgccgc agcagcgact gcacacgcgg gaacagcgtc atgtgcgcgc
     1441 cggagagtat ggagaggccg acgacgtcca cgtcctcctg gatcgccgcc tgggcgatca
     1501 tttcgggagt ctggtgcagg cccgtgtaga tcacctccat accggcatcg cgcagcgccg
     1561 cggctacgac cttcgcgccg cggtcgtgcc cgtccagtcc cggcttggcg accagcacac
     1621 ggatcgggcg ccgcttgcgg ccgtcgacgg cgaggctcat gcgcggcgga accggagcag
     1681 cgcggcgagg ccgtccacgc cccggcgcgc acggtcgtcg ttcagcacgt ccacctgggc
     1741 gcgttgcgcc agcgcctcct ccaccgcgtc gtcgagacgg acatcctcgt cccgcccgtc
     1801 caccagcagc gtccgcacct gaccgcgcgc cagcgccttc tccgtcgcct cgatcccgtt
     1861 gacggcccac cccgtcccca aaccgtcctt cacggcttcc gcgtgggcgc gctcccaggc
     1921 gcgctcgcgc tcctcgcgta acgccagggc cgcctctcgc acctccgccg gcgtgatggt
     1981 cttgggattg agccgcacga cgccgaacag caggtcgtgc aggtaggtgt ggaggtgcgg
     2041 caccagggcg gcggcatcca ccccgatccc cgccacgacg agtccggaca gcggcgtctg
     2101 gacattgatc cggaagatct cctcggcgac gcgcgcgtag tggcggtgct tttcctcccg
     2161 gatgcgcccg tggtgcccgt gctccccggc cgagccgggc acggcgccgc gcatgacctg
     2221 ccgctcgccg tggaatttct cggtgcggga cgccggggag gtgatccccg agcgctccgt
     2281 gatgccgaac gcggtcaccg tgaagaaccg ggcccccgtg cggtcgcagg cggcgacgag
     2341 aatcgtcccg aactcctgct ccagcgcgac cagctcccgc gtggccgggg cgtcggcgac
     2401 gaccaggcgc gagcgctgca cctgcgggag gggaatcacc tcgaacaggt ccagcgcgcc
     2461 gcaggcgaac agggcgaccc cgcgggcctg gggcagccgg ccgggttcct cgaggtaacg
     2521 aagcacgcga tccaggtcgg cggccaccgc ctcccgctcg ctgcggttga gcgcgcgttg
     2581 cgccagaccg acggcggtct ccttcacgcg gttcttcatc ttgatgaggt acttgccgcg
     2641 ggccttgtcg cgcggctcga gcttcaggta gcacgagacg acctgccggc cacccggcgt
     2701 gatcctgccg aggcgatcca gcacttcgtt gaccgtgccc ttccccgggg agaacggcag
     2761 cgccgccatc agaagaacac cggttcgcgg taggcgccgt acacgcgctc gagcgcgtgc
     2821 cggatctcgt acagcgtgca gtagacgcgg gcacaatcca gcatcggccc gatcacgttg
     2881 tggtcgttct cggcggcctg ccgcagggcc tcgagccggc ggtcgacgag cgcctgatcg
     2941 cgcccggccc gcagctcgac catgcgccgc cgctgcgagt cctctgcatc gttggtgatg
     3001 cgcaggacct cgacgggatg atcctcctcg gagaggaagt cgttcacccc gacgatgact
     3061 ttccgctgct cctccacctg gcgctggaat tccgccgcgg agcgggcgat ctggcgctgg
     3121 aaccagccgt tttcgatccc cgacacgacc ccgccgatcg actggatctc cgcgaacagc
     3181 gcctccgctt cccgctcgag ctgatcggtg agcgactcca gatagtagga accgcccagc
     3241 ggatcgatca cttccggcac gccggtctcg tgtgcgagga tctgctgcgt gcgcagcgcg
     3301 acctgcaccg cctgctcggt cggcagggcg agcgtctcat ccagcgaatt ggtgtgcaac
     3361 gactgcgtgc cgcccagcac cgccgccagt gcctggtaag cgacgcgcac gatgttgttc
     3421 atgggctgtt gcgccgtgag cgtcaccccc gcggtctggg aatggaaccg cagcttcagg
     3481 gagcggtcgc tcacggcgcc gtagcgttcc cgcatgtgac gcgcccagat gcgccgcgcc
     3541 gcgcgcagct tcgcgatttc ctcgaagaaa tcgttgtgaa tgtcccagaa gaacgacagg
     3601 cgcggcgcga agccgtcgac gttcagcccg cgcgccacgc cgcgctcgac gtaggtgaag
     3661 ccgttggcca gcgtgaacgc cagctcctgc gccgccgtcg cccccgcctc gcggatgtgg
     3721 tagcccgaga tcgagatggt gttccattcc ggcgtgtgct cggcgcacca ctcgaacatg
     3781 tccacgatga tcttcagcgc cggctccacc gggtacaccc aggcgtgctg cgccatgtac
     3841 tccttgagga tgtcgttctg caccgtgccg cgcagcttcg cgatcggcac gccctggcgc
     3901 tcggcggcgg ccacgtagaa acagaacaga atcgccgccg gcccgttgat ggtcatcgag
     3961 gtcgagacct ggtcgagcgg aatgccatcg aacagcgtct gcatgtccgc gaggctcgag
     4021 atcgcgacgc cgcacttccc cacctcgcct tcggcacggg gatgatcgga gtcgtagccc
     4081 attagcgtgg ggaaatcgaa ggccaccgac aggcccgtct ggccgcgcgc cagcaggaac
     4141 ttgtagcggc gattggtatc cgctgcggtc ccgaagccgg cgaactggcg catggtccac
     4201 agccggccgc ggtacatcgt gggatggatc ccgcgggtga acgggtacgc gccgggcgtg
     4261 cccagctgcg ccgcggggtc ccaccccgcg aggtcccggg ctccatagac cggcttccgc
     4321 tcgatgctca tcgcgtggcc cccgttctcg cgtgatccac gatggccgcg gccacctcgt
     4381 aaggactccg cttgcccgcc gacatgtcgt ccagcgcctc ctcgagcagg gtctccgcgg
     4441 cagtctgctc ccatacccag tgcgccaggg cgcggttcac cgacgcccgc gtccgctcgg
     4501 cgagtcgctc ccgccgccga cgctcgagca ggccgctcgc ttcaaggtac ccacggtgcc
     4561 gctccagtgc ctccaccagc ccggtcacgc cctcaccacg ggtggcgacc gtcagcagga
     4621 ccggcggctc ccacgtcggc gggggggggg gggg
//
LOCUS       DBNGZW010000017         3023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_956405, whole genome shotgun sequence.
ACCESSION   DBNGZW010000017 DBNGZW010000000
VERSION     DBNGZW010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3023
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_956405"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1467
                     /locus_tag="AC16JC_00445"
     CDS             <1..1467
                     /locus_tag="AC16JC_00445"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR5163192.1"
                     /translation="LIGFYDDNRITIDGATDLTFSDDTARRFEAYGWHVQHVADGNDL
                     AAIDHAIRHAQAATDRPSLIILRTHIAYGSPNKQDTAEAHGAPLGAAEVKLTKEALGW
                     PSLEPFVVPEQALTEWRRARQRGALLHADWQARWSDYRAAHPELAAELERRLAGRLPD
                     GWDSAPPAFAATDAQATRAASGKVLNAIAPRLPELIGGSADLTGSNLTHIKGAEDFAP
                     GLVTGRNFHFGVREHAMGAVLNGLAYHGGFIPFGGTFLIFSDYMRPAIRLAALAQLKV
                     IYVFTHDSIGLGEDGPTHQPIEQLAALRAIPNMTVIRPSDPTETVEAWRAAIRHERGP
                     VALVLTRQKVTVIDRSRHAAATGLHRGGYVLAEAPGGQPEVMLLATGSEVEIALGAFD
                     RLGAEGVRARVVSMPCLEYFALQPQAYRDEVLPPAVTRRIAIEAAAPQSWYRWVGERG
                     VIVGIDRFGASAPYQRIYREFGLTVENVVAQARSLIKV"
     gene            complement(1469..2530)
                     /locus_tag="AC16JC_00450"
     CDS             complement(1469..2530)
                     /locus_tag="AC16JC_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163193.1"
                     /translation="MRWIGVVVLASAACAPRSPSRDPEPTAVLTLQSQSSGTTALLQA
                     VSAPSDRVAWVSGHGAAVLRTVDGGATWERITVPGAAADSLQFRDVHAVNEAVAYLLA
                     AGPGNRSRIYKTTDGGGTWQLQFTNADSSAFYDCFAFWDASHGLVFSDAVAGHFVIRV
                     TTDGGAHWTPVPVDVLPPAQPGEGAFAASGTCVVTLGTNEAWVGTGAADTARVLRTPD
                     GGRSWDVAATPIAGGRTAGIAALAFRDSLHGIAMGGDVADADARTDNVAVTADGGRTW
                     RGGGRPGFSGAVYGAATVPGRPGWVVVVGPKGLAYSTDDGASWTNVDTLAYWSVGFGS
                     RRRGWAVGPTGRIVEIRMQ"
     gene            complement(2533..>3023)
                     /locus_tag="AC16JC_00455"
     CDS             complement(2533..>3023)
                     /locus_tag="AC16JC_00455"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HIR5163194.1"
                     /translation="LYTSLKRRSTLNTLVGAVPGALPVLGGWTAAGGALDAPGLALFW
                     TLFLWQLPHFLALAWMYREDYRSARLKMLSVEDTDGRRTARMALLYAVALLPVSLLPT
                     MLGVTGTLYFWGALALGLAYVGASVFMLVDVNRRAWWVFLASIIYLPALLTLAVLDKV
                     SV"
BASE COUNT          480 a         1136 c          996 g          411 t
ORIGIN      
        1 ctgatcggct tctacgacga caaccgcatc accatcgacg gcgccaccga cctcacgttt
       61 tccgacgaca cggcccggcg gttcgaagcc tacggctggc acgtgcagca cgtggctgac
      121 ggcaacgacc tcgccgccat agaccacgcc atccggcacg cgcaggccgc caccgaccgc
      181 ccctcgctga tcatcctgcg cacccacatc gcctacggca gccccaacaa gcaggacacc
      241 gccgaagcgc acggcgctcc gttgggcgcg gcggaagtga agctgacgaa ggaagccctc
      301 ggctggccgt ccctggagcc gttcgtggtt cccgagcagg cgctcaccga gtggcggcgc
      361 gcccggcagc ggggtgcgct gctgcacgcc gactggcagg ctcgctggtc cgactaccgc
      421 gccgcacacc ccgagctcgc ggcagagctc gagcgtcggc tcgcgggacg gcttcccgac
      481 ggctgggact ccgccccgcc ggcgttcgcc gcgaccgacg cccaggcgac ccgcgccgcc
      541 tccgggaagg tattgaacgc catcgccccg cggctccccg agctcatcgg cggatcggcg
      601 gacctcacgg gctccaacct gacgcacatc aagggggcgg aggacttcgc acccggcctc
      661 gtcaccgggc gcaactttca cttcggcgtg cgcgagcacg cgatgggcgc ggtgctgaac
      721 ggtctcgcgt atcacggtgg attcattccg ttcggcggga cgttcctcat cttctccgac
      781 tacatgcggc cggccattcg gctcgccgcg ctcgcgcagc tcaaggtgat ctacgtgttc
      841 acccacgact cgatcggcct gggagaagac ggcccgacgc accagccgat cgagcagctc
      901 gccgccctgc gcgcgatccc caacatgacg gtgatccgcc cctccgaccc gaccgaaacg
      961 gtggaggcgt ggcgggccgc gatccgccac gagcgcggtc cggtcgccct ggtgctcacg
     1021 cgccagaagg tgacggtgat cgatcggtcc aggcacgccg ccgccaccgg cctgcaccgt
     1081 ggcgggtacg tgctcgcgga ggcccccggc ggccagcccg aggtcatgct gctcgcgacc
     1141 ggatcggaag tggagatcgc cctcggcgcg ttcgaccgac tcggcgcgga aggggtccgc
     1201 gcgcgtgtgg tcagcatgcc gtgcctcgag tatttcgcgc ttcagccgca agcctaccgc
     1261 gatgaagtcc tcccccccgc ggtcacgcgg cgcatcgcca tcgaagccgc ggcgccgcag
     1321 tcatggtacc gctgggtcgg tgagcgcggg gtgatcgtgg ggatcgatcg gttcggcgcg
     1381 tcggcgccct atcaacggat ctacagagaa ttcggattga cggtcgagaa cgtggtagcg
     1441 caggcgaggt ctctgatcaa ggtctgagtc attgcatccg gatctccacg atcctccctg
     1501 tcggcccaac agcccaccct ctccgtcgcg agccgaagcc cacgctccag tacgccagcg
     1561 tgtccacatt cgtccagctc gcgccgtcgt cggtcgagta ggcgagcccc ttggggccaa
     1621 ccacgaccac ccaccccggg cgcccgggca cggttgcggc gccgtacacc gccccgctga
     1681 accccggccg gccacccccg cgccacgtcc ggccgccgtc cgccgtgacc gccacgttgt
     1741 cggtccgcgc atcggcgtca gcgacatccc cgcccatagc gatcccgtgc agagaatccc
     1801 tgaacgccag cgccgcgatc cccgctgtgc gacctccggc aatcggcgtt gccgcgacgt
     1861 cccaggagcg ccccccatcg ggcgtgcgca gcacgcgggc ggtgtcggcc gctccggtgc
     1921 ccacccacgc ttcgttcgtg ccgagcgtga cgacgcacgt gccgctcgcc gcgaacgcac
     1981 cctcccccgg ctgcgcagga ggcaatacat ccaccgggac gggcgtccaa tgcgccccgc
     2041 cgtccgtcgt cacgcggatc acgaagtgcc ccgccacggc gtcgctgaag acgaggccgt
     2101 gcgatgcgtc ccagaacgcg aagcagtcgt agaaggcgct cgagtcggcg ttcgtgaact
     2161 gcagctgcca ggtgccgccg ccgtccgtgg tcttgtagat gcgcgagcgg ttgccaggcc
     2221 ccgcggcgag gagataggcg acggcctcgt tcaccgcgtg cacgtcgcgg aactgcagcg
     2281 aatccgccgc tgcgcccggc acggtgatgc gctcccacgt tgcgccgccg tccacagtcc
     2341 gcagcaccgc ggcgccgtgg ccgctcaccc aggcgacacg atcgctcggc gcgctcactg
     2401 cctgcagcag cgccgtcgtc ccgctcgact gcgactggag cgtgaggacg gcggtcggct
     2461 ccggatcgcg gctcgggctc cgcggcgcgc atgccgcgct ggccaggacg accacaccga
     2521 tccaccgcat cgtcagacgc tgaccttgtc gagcaccgcg agcgtcagca acgcgggcag
     2581 atagatgatg ctggcgagga acacccacca cgcccgccgg ttcacgtcca ccagcatgaa
     2641 gacgctggcg ccgacgtacg cgagaccgag cgcgagcgcg ccccagaagt agagggtgcc
     2701 ggtgacgccg agcatggtgg ggagcaggct cacggggagc agcgccacgg catagagcag
     2761 ggccatccgg gctgtgcgcc gcccgtcggt gtcctctacc gacagcatct tgagccgtgc
     2821 gctgcggtag tcctcgcggt acatccaggc aagcgcgaga aaatggggga gctgccacag
     2881 gaacaacgtc cagaataacg cgagtccggg cgcgtcgagc gcaccccccg cggcggtcca
     2941 gccgccgagt accggaaggg cccccggaac ggcgcccacc agggtattca acgtgctccg
     3001 tcgcttgagc gacgtataga gcc
//
LOCUS       DBNGZW010000018         5287 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_69548, whole genome shotgun sequence.
ACCESSION   DBNGZW010000018 DBNGZW010000000
VERSION     DBNGZW010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5287)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_69548"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="AC16JC_00460"
     CDS             complement(<1..256)
                     /locus_tag="AC16JC_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163195.1"
                     /translation="MLELPALAVLLLSPQTPHGSRATDSTSVAPPTGMTARAVRAAQP
                     PAIDGRSSDAIWQGPPTISDFQEWSPTEGKSPRFPTEARIA"
     gene            444..647
                     /locus_tag="AC16JC_00465"
     CDS             444..647
                     /locus_tag="AC16JC_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5163196.1"
                     /translation="MNRLRVARAEKRLSQEQLAQLAGVTRQTIGAIENGQYYPSALLA
                     FVLARRLETPVTELFLLEEEEKL"
     gene            644..943
                     /locus_tag="AC16JC_00470"
     CDS             644..943
                     /locus_tag="AC16JC_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163197.1"
                     /translation="MNAGHVERDERTVAVENAGYRLSYHALSFGLLGLTAYRSFARGE
                     SAWDLLALVVLGGVLNAAYQGWRRVLYRRWVVMTLVTFAIAALVAAGMVIVRNAR"
     gene            1013..1891
                     /locus_tag="AC16JC_00475"
     CDS             1013..1891
                     /locus_tag="AC16JC_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015940443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5163198.1"
                     /translation="MHDLTLTVPAGLIFGLLGPNGAGKTTTIRLLLGLLEPSSGRARV
                     LGWDAQTQSEAIRTHTGALLEHTGLYERLSAEQNLEFYGRIYRLPRAKRRERIKDLLS
                     HLGLWDRRADPVAVWSRGMRQRLAIARAMLHRPRLLVLDEPTAGLDPIAAAALREELA
                     GLVAREGVTMFLTTHNLAEAESLCDRVAVLRNGRLLAAGTPREIRAGSGRPRVEIAGR
                     GFGSEILDRLRSRPEVKVVEVVEDGSTLAIELHPHADVAALVALIVSQGGAVEQVRRD
                     DPGIEEAFRSLVLEAP"
     gene            1888..2622
                     /locus_tag="AC16JC_00480"
     CDS             1888..2622
                     /locus_tag="AC16JC_00480"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5163199.1"
                     /translation="MTGDVWTMAWKEWQELFAAQGRFRGGAVNFSLLLGVFGIALPWQ
                     FGRAWVTSPMMLLYWSWAPLLLLSHVVVDAIAGERERHTLETLLASRLPDRAMLFGKM
                     AAAIAYGLAVTAGSLALGLVTVNLRDGNGGLVVYPPATLSGALVTTVLAATMIAAIGV
                     LVSLRAASVRQAGQIMGAAVLLVAFVPVVAVRALVGRWHAPGHPFSAAETLVLGVVIL
                     ATLALVDVALVAAAMLRFRRSRLALD"
     gene            complement(2628..4667)
                     /locus_tag="AC16JC_00485"
     CDS             complement(2628..4667)
                     /locus_tag="AC16JC_00485"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014681192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HIR5163200.1"
                     /translation="MAPTPRLLALSLAAALCAAAPLRSQVSVASPDGRNRVTVEIREG
                     RLYYSLARDGHALILPSLLGFAFRGAPPLRDSLRIADTTRQSHDEWWTQPWGEVARVH
                     DHHNELAVSVQETGAPNRRFILRVRAFDDGVGFRYEIPAQPGLGAFEISDELTEFAMA
                     DNARAWWIASNWPRKDRSEELFSSGPVSTLDSVQTPLTLELRDGHTVMVIHEANLVDY
                     ARMFLAGPRMEGRTLHAALAPWADGVKVRGHTPLVTPWRTIQVADRVTQLAPSLLGLN
                     LNPPNVLARADWIHPMKYVGIWWGMHIGAMTWSSGPKHGATTANAKRYIDFAAANGFG
                     GVLVEGWNTGWDGDWIANRDAFSFTTSYPDYDLAEVARYAKSKGVRLIVHNETSGGID
                     NYERQLDSAYALYRSLGLDAIKSGYVTDLTSEGHSHYSQYMVRHYRHVIEEAAKHGIM
                     LDVHEPMHDTGERRTYPNMMSREGARGQEYNAWSGDGGNPPEHETILFFTRLLDGPMD
                     FTPGIFDLLERSSGRAHGPEEPRVRTTLAKQLALYVVLYSPLQMAADLPENYANQPAF
                     QFIRDVAVDWDTTRVIDGRIGDYVVVARRERGGNTWYLGAITDEEGRTFDIPLSFLER
                     GRRYVAEIYADGPGANWLTNPFPVAISRRPVTAASRLHIVLAPGGGQAVRIRPAP"
     gene            4857..>5287
                     /locus_tag="AC16JC_00490"
     CDS             4857..>5287
                     /locus_tag="AC16JC_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5163201.1"
                     /translation="MPSFSLTVNGRRRTVTAPADMPLLWVIRDLLGLTGTKFGCGIGQ
                     CRACTVHLDGSATFSCRTPISAAAGHRVTTIEGLSPARLHPVQQAWIDEDVPQCGYCQ
                     PGQIMSAAALLAKNPKPTDAQIDEAMMGNICRCGTYNRIRRA"
BASE COUNT          759 a         1789 c         1862 g          877 t
ORIGIN      
        1 aggcgatcct cgcctcggtg ggaaagcgcg gcgacttccc ttccgtgggg gaccactcct
       61 ggaaatcgct gatggtcggg gggccttgcc agatcgcgtc gctgctgcgc ccgtcgatgg
      121 ccgggggctg cgcggcccgc accgcccggg cggtcatccc tgtgggcggc gcgacgctcg
      181 tgctgtcggt cgcgcggctc ccgtgtgggg tctgcggact cagcagcagg acggcaaggg
      241 ccggcaactc gagcattcag cctcggacgg cggagaacct cctctacggt ggcagggggc
      301 gtgggtttcg cgacccgcct cccctccgca tctctaagtc atctgacaag tatacagtac
      361 agtatgttat gtatacttga caatgtgtcc gtcataccat acaatacgtg aacgaatccg
      421 atctcggggg cggtttgcgg ttagtgaacc ggttgcgcgt cgcgcgtgct gagaagcggc
      481 tgtcgcaaga gcagctcgcc cagctggcgg gcgtcacgcg gcagacgatc ggcgcgatcg
      541 agaacgggca gtactacccg tcggcgctgc tggcattcgt gctggctcga cggctcgaga
      601 caccggtcac cgagctcttc ttgctggagg aggaggagaa gctatgaatg ccggacacgt
      661 cgagcgagac gagcggacgg tggcggtgga aaacgccggc taccgcctgt cctaccacgc
      721 gctgtcgttc ggtctgctgg gactcaccgc gtatcgctcc ttcgcgcgcg gcgaatcggc
      781 gtgggacctc ctggccctcg tggtgctcgg tggcgtgctg aacgcggcct atcaggggtg
      841 gcgtcgcgtg ctgtaccggc gctgggtcgt tatgacgctc gtgaccttcg cgattgccgc
      901 gttggtggct gccgggatgg tgatcgtgcg gaatgcccga tgagcatcgc cgccggcgcg
      961 ctgggcgatc gagaccgagg cgctgtgccg cgacttccgg ggccggcgag cagtgcacga
     1021 tctcacactc accgtccccg ccggcctcat cttcggactg ctcggaccca acggggccgg
     1081 caagaccacc accatccgcc tgttgctcgg tctgctggaa ccctcgagcg ggcgcgcccg
     1141 cgtgttgggt tgggacgcac agacgcagag cgaagcgatt cggacgcaca cgggcgcgct
     1201 gctcgaacac accggtctct acgagcgcct cagcgccgag cagaacctgg agttctacgg
     1261 ccggatctac cgcctgccgc gggcgaaacg acgcgagcgg atcaaggatc tgctcagcca
     1321 tctgggcctg tgggaccggc gcgccgaccc ggtcgcggtc tggagtcgcg ggatgaggca
     1381 gcgcctggcg atcgcccgcg cgatgctgca tcgcccccgg ctgctcgtgc tcgacgagcc
     1441 caccgccggt ctggacccga tcgccgccgc ggccctgcgg gaagagttgg cgggtctcgt
     1501 cgctcgagag ggcgtcacga tgttcctcac gacccacaac ctcgccgagg cggagagtct
     1561 gtgcgaccgc gtggccgtgc tccggaacgg gaggctgctg gcggccggga cgccgcgtga
     1621 gatccgggcg gggagcggcc gtccgcgcgt ggagatcgcg ggtcgcggtt tcggctcgga
     1681 gatcctcgac cggttgcgca gccggcccga ggtgaaggtg gtggaggttg tggaggatgg
     1741 ttccaccctg gcaatcgagt tgcatcccca tgcggacgtc gcggccctcg tcgccttgat
     1801 cgtcagtcag ggaggcgccg tggagcaagt gcgccgcgac gatcccggca tcgaggaggc
     1861 gttccgctcg ctggtattgg aggccccatg accggtgacg tatggacgat ggcgtggaag
     1921 gagtggcagg agctcttcgc ggcacagggg cgattccgcg gcggcgcggt caacttctcg
     1981 ctgctgctcg gagtcttcgg gatcgccctg ccctggcagt tcggtcgcgc atgggtgacc
     2041 tctcccatga tgctcctgta ctggagctgg gctccgttac tgctgctctc ccacgtggtc
     2101 gtggatgcca tcgccggtga gcgcgagcgc cacaccctcg agacgctgct cgccagccgc
     2161 ctgccggacc gggcgatgct cttcggcaag atggccgcgg ccattgcata tggcctcgcc
     2221 gtcaccgcgg gaagtctcgc gctgggcctg gtgacggtga acctgcgcga cgggaacggt
     2281 gggctggtgg tctacccgcc ggccactctg agcggcgcgc tggtcaccac ggtgcttgcc
     2341 gcgacgatga tcgcggccat cggtgtgctg gtgtcgctcc gtgccgccag cgtgcgccag
     2401 gcggggcaga tcatgggggc cgccgtgctg ctcgtcgcgt tcgttccggt ggtcgcggtc
     2461 cgtgccctcg tgggccggtg gcacgctccg gggcacccgt tcagcgcggc cgagacgctg
     2521 gtgctcggcg tagtcatcct ggctaccctg gcactggtcg acgtcgccct cgtcgccgcg
     2581 gccatgctgc gctttcgacg ctcgcggctg gcgctggact aggcgcccta cggcgccggc
     2641 cggatccgga ccgcctgccc tcccccgggg gccagcacga tgtgcaggcg tgacgccgcg
     2701 gtgaccggcc gccgcgagat cgcgacgggg aacggattgg tgagccagtt cgcccccggc
     2761 ccatcggcgt agatctcggc cacgtacctg cggccgcgct cgaggaacga gagcgggatg
     2821 tcgaaggtac gtccctcttc gtccgtgatg gcaccgaggt accaggtgtt gccgccccgc
     2881 tcccggcgcg cgaccaccac gtagtcgccg atccggccgt cgatcacccg ggtcgtgtcc
     2941 cagtccacgg cgacgtcacg gatgaactgg aacgcaggct ggttcgcgta gttctcgggg
     3001 aggtcggccg ccatctgcag cggcgagtag agcacgacgt acagcgccag ctgcttcgcc
     3061 agcgtggtgc gcacccgcgg ctcctcgggc ccgtgcgcgc gcccgctcga ccgctccagc
     3121 agatcgaaga tccccggcgt gaaatccatg ggaccgtcga gcagccgcgt gaagaagaga
     3181 atggtctcgt gctccggcgg attgccgccg tcgccgctcc aggcgttgta ctcctgcccc
     3241 cgcgcgccct cgcggctcat catgttgggg taggtgcggc gctcaccggt gtcgtgcatc
     3301 ggctcgtgca cgtccagcat gatgccgtgc ttcgcggcct cctcgatgac atggcggtag
     3361 tgccgcacca tgtactgcga gtagtgcgag tggccctcgc tcgtcaagtc ggtgacgtac
     3421 ccgctcttga tggcgtcgag gcccagcgag cggtacagcg cgtacgcgct gtccagctgg
     3481 cgctcgtagt tgtcgatccc acccgacgtc tcgttgtgta cgatgagccg cacgcccttc
     3541 gacttggcgt agcgggcgac ttccgcgaga tcgtagtcgg gataggacgt ggtgaacgaa
     3601 aacgcgtcac ggttggcgat ccagtcgccg tcccagccgg tgttccaccc ctcgaccagc
     3661 accccgccaa acccgttcgc cgccgcgaaa tcgatgtacc gtttcgcgtt ggccgtcgtc
     3721 gccccatgct tgggccccga gctccaggtc atggcgccga tgtgcatccc ccaccagatg
     3781 cccacgtact tcatcgggtg aatccagtcg gcgcgcgcca ggacgttcgg cggattgagg
     3841 ttgaggccga gcagcgaggg cgcgagctgc gtgacgcgat cggccacctg aatcgtgcgc
     3901 cacggcgtca cgagcggcgt atggccgcgc accttgacgc cgtcggccca gggcgccagg
     3961 gccgcatgca gggtgcggcc ctccatgcgt ggccccgcca ggaacatgcg cgcgtagtcc
     4021 acgaggttcg cctcgtggat gaccatcacc gtgtggccgt cccgcagctc cagcgtgagc
     4081 ggcgtctgca cgctgtccag ggtgctcacg ggccccgaag agaacagctc ctcggaccgg
     4141 tccttgcgcg gccagttgga cgcgatccac caggcgcgcg cgttgtccgc catggcgaac
     4201 tccgtcagct cgtcggagat ctcgaaggcc ccgaggccgg gctgcgcggg gatctcgtag
     4261 cggaacccca ccccgtcgtc gaaggcgcgg acccggagga tgaaccgccg gttgggagcc
     4321 cccgtttcct gcaccgagac cgccagctcg ttgtgatggt cgtggacgcg ggccacctca
     4381 ccccagggtt gggtccacca ttcgtcgtgc gactgccgcg tcgtgtcggc gatgcgcagg
     4441 ctgtcgcgca gcggcggtgc gccccggaag gcgaacccga gcagcgaggg aaggatcagc
     4501 gcgtggccgt cgcgcgcgag gctatagtac agccggccct cccggatctc gaccgtgacc
     4561 cggttgcggc cgtcgggcga ggcgacgctg acctgggacc ggagcggagc ggcggcgcag
     4621 agcgccgcgg cgaggctgag tgcgaggagg cgtggtgtgg gtgccatgga cctaatgtcg
     4681 ctcccttccg agacacggcg aaaggccacc cggggcgtcg cgtgcggtgg cacgccgccc
     4741 cgtttgcaca ggacgaccgc gccggcattc gcctttggcg tgagcgactg atggcccttg
     4801 acgggcggca gtttgcgacc gagcctatga tcagggaatc ctgccaagga gccgccatgc
     4861 cttccttcag cctcaccgtg aacggccggc gccgcacggt gaccgccccc gccgacatgc
     4921 cgttgctgtg ggtgatccgc gacctgctcg gcctcacggg caccaaattc ggctgcggca
     4981 tcggacagtg ccgcgcgtgc accgtgcacc tggacggcag cgccacgttc tcctgccgca
     5041 cgcccatttc cgcggccgcc ggccaccgcg tcacgaccat cgaagggctc tcaccggctc
     5101 gactgcatcc ggtgcagcag gcctggatcg acgaggacgt gccgcagtgc ggctactgcc
     5161 agccggggca gatcatgagc gccgccgctc tgctggccaa gaatcccaag cccaccgacg
     5221 cgcaaatcga cgaagcgatg atgggcaaca tctgccgctg cggtacgtac aaccgcatcc
     5281 ggcgcgc
//
LOCUS       DBNGZW010000019         3391 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_547485, whole genome shotgun sequence.
ACCESSION   DBNGZW010000019 DBNGZW010000000
VERSION     DBNGZW010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_547485"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="AC16JC_00495"
     CDS             <1..740
                     /locus_tag="AC16JC_00495"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017938616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR5163202.1"
                     /translation="TPNCLEVTMALYQPSIERLVPVSSTEAAELVKILENTFRSVNIG
                     LVNEMAIVCDKLGVDVWEVIDAAATKPFGFMKFTPGPGVGGHCIPLDPHYLAWKMRTL
                     NYRTRFIELAGEINAEMPEYWVARVADRLNDQGKAVRRSKVLVVGVAYKKDIDDIRES
                     PALDVIRLLEQRGAVVSYHDPHVARLHEDGVELTSVPLTAEALRGADCVVIVTDHSSL
                     DYGLIAREAKLVVDTRHALPEETRRPS"
     gene            737..2056
                     /locus_tag="AC16JC_00500"
     CDS             737..2056
                     /locus_tag="AC16JC_00500"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose dehydrogenase family protein"
                     /protein_id="HIR5163203.1"
                     /translation="MRLCVVGTGYVGLVVGACFAETGNDVLCADVDAKKIAGLKENRL
                     PIYEPGLEPLVLRNQRDGRLQFTTDVAAAIEASDVVFIAVGTPPDEDGSADLQHVLAV
                     ARTIGQHMNRPKVVATKSTVPVGTAELVRAEIAKHTKTAFHVCSNPEFLKEGAAVDDF
                     MKPDRVIVGVDHPESRRMFEELYGPFVRTGNPLIFMDIPSAEMTKYAANAMLATRITF
                     MNQVAQLCEAVGADVQLVRKGIGSDHRIGPAFLFPGPGYGGSCFPKDVKALVRTADGK
                     GVKLGVLQAVEDGNERQKRVLYEKLARHFDGKLEGRTIGVWGLAFKAETDDVRESPAL
                     VVVEALLAAGATVQAHDPAAIETARHVLGDRVRYVEHAYDAVAGADALVIVTEWREYR
                     NPDFARLKQALKRPLIVDGRNLYEGTRLAELGFTYDCIGRPLACASS"
     gene            2041..2976
                     /locus_tag="AC16JC_00505"
     CDS             2041..2976
                     /locus_tag="AC16JC_00505"
                     /EC_number="4.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048040 - UDP-glucuronate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HIR5163204.1"
                     /translation="MRILVTGAAGFLGSHLCDRLLADGHRVIGMDNYITGTPANLAHL
                     AHHAAFQFVRHDVTTFIEVDGPLDGVMHFASPASPVDYLELPIQTLKVGSLGTHKALG
                     LARAKRSRFLLASTSEVYGDPLVHPQPESYWGNVNPVGPRGVYDEAKRFAEAMTMAYH
                     RFHGIDTRIVRVFNTFGPRMRPHDGRVVSNFIMQALRGEPLTIYGDGSQTRSFCYVDD
                     LVDGIVRLFERGTSEPTNIGNPGEFTVRQLADRVLALTGSRSQVVQRPLPVDDPRVRQ
                     PDITRARQLLGWEPRISLDEGLTRTIAYFRGVLTT"
     gene            2973..>3391
                     /locus_tag="AC16JC_00510"
     CDS             2973..>3391
                     /locus_tag="AC16JC_00510"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5163205.1"
                     /translation="MSRLLVTGAAGFIGSHVCEALLDAGHEVAGIDNFDPFYARATKE
                     RNVASLRQRRGFLFSEGDVGRDPLPLEGVEAIIHLAAKPGVQTSVQQPAAFMEANVTA
                     TTRLLESAHQEGITRVVFGSSSSVYGESTPAPFPEDAR"
BASE COUNT          563 a         1109 c         1183 g          536 t
ORIGIN      
        1 tcacgccgaa ctgcctcgag gtgacgatgg cgttgtacca gccgtcgatc gagcggttgg
       61 ttccggtctc ctcgaccgag gcggccgagc tcgtgaagat cctcgagaac accttccgca
      121 gcgtgaacat cgggctcgtc aacgagatgg ccatcgtgtg cgacaagctc ggcgtcgacg
      181 tgtgggaggt gatcgatgcc gcggcgacca agccgttcgg gttcatgaaa ttcaccccgg
      241 ggccgggggt gggcgggcac tgcatcccgc tggatccgca ctatctcgcg tggaagatgc
      301 ggacgctgaa ctaccggacg cggttcatcg agctcgcggg cgaaatcaac gccgagatgc
      361 ccgagtactg ggtggcccgg gtggcggatc gcctcaacga tcagggcaag gcggtgcgcc
      421 gcagcaaggt gctggtggtg ggtgtcgcct acaagaagga catcgacgac atccgggaaa
      481 gtcccgcgct cgacgtgatt cgcctgctcg agcaacgggg agccgtcgtt tcgtatcacg
      541 atccgcacgt ggcacggctg cacgaagacg gggtggagct cacgtccgtg ccgctcaccg
      601 ccgaggcgct gcgcggggcc gactgcgtcg tgatcgtcac cgatcactcg tcgctcgact
      661 acgggctcat cgcgcgtgag gcgaagctcg tggtcgatac gcggcacgcc ctgcccgagg
      721 aaaccaggag gccgtcgtga gactttgcgt ggtggggacc ggctacgtgg ggctcgtcgt
      781 gggcgcctgc ttcgccgaga ccggaaacga cgtgttgtgc gcggatgtcg acgcgaaaaa
      841 gatcgccggc ttgaaggaga accggctgcc gatctacgag ccggggctcg agccgctcgt
      901 gctgcgcaat cagcgcgacg ggcggctgca gttcacgacc gatgtcgccg ccgcgatcga
      961 ggcgtcggat gtcgtgttca tcgccgtcgg cacgccgccc gatgaagacg gctcggcgga
     1021 tctccagcac gtcctggcgg tggcgcggac catcgggcag cacatgaacc ggcccaaggt
     1081 cgtcgccacg aagagcaccg tgccggtggg gaccgcggag ctggtgcggg cggaaatcgc
     1141 gaagcatacc aagaccgcgt tccacgtctg ctccaatccc gagttcctga aggaaggcgc
     1201 agccgtcgac gatttcatga agccggaccg cgtgatcgtc ggcgtggatc acccggagtc
     1261 ccgccgcatg ttcgaggagc tgtacggtcc cttcgtgcgc accggcaacc ccctcatctt
     1321 catggacatc ccgtcggcgg agatgaccaa gtacgccgcc aacgccatgc tcgcgacgcg
     1381 catcacgttc atgaaccagg tggcgcagct gtgcgaggcc gtgggcgccg acgtgcagct
     1441 ggtgcgcaag ggcatcggct cggatcaccg catcggcccg gccttcctgt tcccgggccc
     1501 cgggtacggc ggctcgtgct tccccaagga cgtcaaggcg ctcgtgcgga cggccgacgg
     1561 caagggggtc aagctcggcg tgctgcaggc ggtcgaagac ggcaacgaac ggcagaagcg
     1621 tgtgctgtac gagaagctgg cgcggcactt cgacggcaag ctcgagggcc gcaccatcgg
     1681 cgtgtggggt ctcgcgttca aggcggagac cgacgacgtg cgcgagagtc cggcgctggt
     1741 agtggtcgaa gcgctgcttg cggccggcgc cacggtgcag gcccacgacc cggccgcgat
     1801 cgagaccgca cggcatgtgc tcggagatcg ggtgcggtac gtggagcacg cctacgacgc
     1861 ggtcgccgga gcggacgcgc tggtgatcgt gacggagtgg cgggagtacc gcaacccgga
     1921 cttcgcgcgc ctgaagcagg cgctcaagcg ccccctcatc gtggatggcc ggaacctgta
     1981 cgaaggcacg cgtctcgccg agctcgggtt cacctacgac tgcatcggcc gcccgctcgc
     2041 atgcgcatcc tcgtaacggg ggcggcggga ttcctcgggt cgcacctgtg cgaccgtctg
     2101 ctcgcggatg gacaccgcgt catcgggatg gacaactaca tcaccgggac gcccgcgaac
     2161 ctggcgcacc tggcgcacca tgccgcgttc cagttcgtgc gccacgatgt gaccaccttc
     2221 atcgaagtgg acggcccgct ggacggcgtg atgcacttcg ccagcccggc gagccccgtg
     2281 gattacctgg agctgcccat ccagaccctg aaggtgggct ccctggggac gcacaaggcg
     2341 ttggggctgg cgcgggcgaa gcgctcgcgg tttctgctcg cgtccacgtc cgaggtatac
     2401 ggcgatccgc tcgtgcatcc gcagcccgag agctattggg gaaacgtcaa tccggtcgga
     2461 ccgcgcgggg tgtacgacga ggcgaagcgc ttcgccgaag cgatgaccat ggcataccat
     2521 cgctttcacg ggatcgacac gcggatcgtg cgcgttttca acacgttcgg gccgcggatg
     2581 cgcccgcatg acgggcgggt cgtctcgaac ttcatcatgc aggcgttgcg gggggagccg
     2641 ctcacgatct acggtgacgg atcccagacg cgctccttct gttacgtgga cgacctggtc
     2701 gacggcatcg tgcggctgtt cgagcggggc acctcggagc ccaccaacat cggaaacccc
     2761 ggggaattca ccgtgcgcca gctcgccgac cgcgtgctcg cgctcaccgg cagccggagt
     2821 caggtcgtgc aacggcccct gccggtagac gatccgcgcg tgcgccagcc cgacatcacc
     2881 cgcgcgcggc agctgttggg gtgggagccc aggatttccc tcgacgaggg cctgacccgg
     2941 acgatcgcct atttccgtgg agtcctgacc acatgagcag actgctggtc accggcgccg
     3001 ccggcttcat cggatcgcac gtgtgcgagg cactcctcga cgccgggcac gaggtggccg
     3061 gcatcgacaa cttcgatccg ttctacgccc gggcgacgaa ggagcgcaac gtcgccagcc
     3121 tgcggcagcg gcgggggttt ctgttctccg agggcgacgt gggtcgcgac ccgctgccgc
     3181 tggaaggggt cgaggcgatc atccacctgg ccgccaaacc cggcgtccag acgtcggtgc
     3241 agcagcccgc cgccttcatg gaagcgaacg tgacggccac cacccgtctg ctcgagtcgg
     3301 cccaccagga ggggatcacg cgggtggtct tcggatcttc ctcttcggtg tacggcgagt
     3361 ccacccccgc gccgttcccg gaagatgccc g
//
LOCUS       DBNGZW010000020         2065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_888563, whole genome shotgun sequence.
ACCESSION   DBNGZW010000020 DBNGZW010000000
VERSION     DBNGZW010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_888563"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1310
                     /locus_tag="AC16JC_00515"
     CDS             <1..1310
                     /locus_tag="AC16JC_00515"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF020892.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR5163206.1"
                     /translation="APQAWVDRMADTFAGFWRRLECSHDDWIRTTEPRHVRAVTALLQ
                     RIQSRRPDDLFEAEYEGLYCTGCEEFKQGSQIVDGRCIEHPTLDLVPRRERNHYFRLS
                     AYRDRVLQLITDGTFAVEPAIRRNEVLRLLEGGLQDISISRQRLPWGIPYPGDPQQTV
                     YVWFDALINYLTATGFPDPGYEERWPADLHVIGKGITRFHCVVWPAMLLAADVALPHR
                     VWAHGYVQWGGAKMSKTAGTVVNVDEAIGRHGPDALRYFLLREIGFENDGNFTWERFD
                     ARYTADLADTFGNLASRTLSMVQRYRGGVVPHDPTAFATPLEHDARHILAAYTGAMDA
                     LALDEGAAQLIALATRANRYVEETAPWKLAKEQRNAELDTVLANLARTVARLAGLALP
                     FMPEKAAQLWDALGTGRELGSLEPLDPSGWTVRKPEPLFPKEP"
     gene            1542..>2065
                     /locus_tag="AC16JC_00520"
     CDS             1542..>2065
                     /locus_tag="AC16JC_00520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163207.1"
                     /translation="MEVSHAVLKTLVGIGGVIVLLLLVLVGAALSRGVSITKGNMLER
                     ENRVLADEISRMRSRLAGLHDTLSTINQRAQELRILAGLPPTDSSVVEAGIGGPVGTW
                     SERDSLTASGALGAQALGAREDLDGLTRRANILVRSINEAYDSLSTHQARMAATPSIM
                     PTKGWLTSAFAAERV"
BASE COUNT          321 a          724 c          712 g          308 t
ORIGIN      
        1 tcgcgccgca ggcctgggtg gaccgcatgg ccgacacgtt cgccggcttc tggcgccgcc
       61 tggagtgcag ccacgacgat tggatccgca cgaccgagcc gcggcacgtc cgcgccgtga
      121 ccgcgctgct gcagcgcatt cagagccgcc ggcccgacga cctgttcgaa gccgagtacg
      181 aggggctgta ctgcaccggg tgcgaggagt tcaagcaggg gagtcagatc gtcgatggcc
      241 ggtgcatcga gcaccccacg ctcgacctgg tgccgaggcg cgagcgcaac cactacttcc
      301 ggttgagcgc ctatcgcgac cgcgtgctgc agctgatcac cgacgggacc ttcgccgtgg
      361 agcccgcgat ccgccgcaac gaggtgctgc ggctgctgga gggcggtctg caggacatct
      421 ccatctcgcg ccagcggctg ccatggggca tcccgtatcc cggcgacccg cagcagaccg
      481 tctacgtgtg gttcgacgcg ctgatcaact acctcaccgc gaccgggttc cccgacccgg
      541 gctacgagga gcgctggccg gccgatctcc acgtgatcgg caaggggatc acgcgcttcc
      601 actgcgtcgt ctggccggcg atgctccttg cggccgacgt cgcgctgccg caccgggtgt
      661 gggcgcacgg ctacgtgcag tggggcggcg cgaagatgag caagacggcc ggcacggtgg
      721 tgaatgtcga cgaggcgatc ggccgtcacg gacccgacgc gctgcgctac ttcctgctgc
      781 gcgagatcgg gttcgagaac gacggcaact tcacctggga gcgcttcgac gcgcgctaca
      841 ccgcggacct ggccgacacg ttcggcaatc tggcgtctcg caccctgtcg atggtgcaac
      901 gctaccgcgg cggcgtcgtg ccgcacgatc cgacggcgtt cgcgacgccg ctcgagcacg
      961 acgcccgcca catcctcgcc gcctacacgg gcgccatgga cgcgctcgcc ctcgacgagg
     1021 gggccgcgca gctcatcgcg ctcgccacgc gcgccaatcg ctacgtcgag gagaccgcgc
     1081 cctggaagct cgccaaggag cagcgcaacg cggagctgga caccgtgctg gcgaacctgg
     1141 cccgcaccgt cgcccgcctc gccgggctgg cgctgccctt catgccggag aaggcggccc
     1201 agctgtggga cgccctgggc accggccggg agctggggag cctcgagccc ctcgacccct
     1261 cgggctggac cgtcaggaag cccgagccgc tcttccccaa ggagccctga cgggctcggg
     1321 cctcaaaagc cctgacggga gcagggaaat tcgagcaaca cacggcccag gaccgtgtga
     1381 cctggctcac gttttgcgtt gttgacggcg gaggatcgcg gggtctatta ttgccttccc
     1441 taaaaccgac agaactagca actcggatac gtatgcgacg cagctctgat cgcaagtgga
     1501 cggtgatggt tgtgcctcac ggctcgggtt cgtcccgcgc cgtggaggta tcccacgccg
     1561 tcctcaagac cctcgtcggc atcgggggcg tgatcgtgct gctgttgctc gtgctggtgg
     1621 gcgccgcgct gtcgcggggc gtgagcatca ccaaggggaa catgctggag cgcgagaacc
     1681 gggtgctcgc cgacgagatc tcgcggatgc gctcgcgtct ggccggtctg cacgacacgc
     1741 tgtcgacgat caaccagcgg gcgcaggagc tgcgcatcct ggccggactc ccccccaccg
     1801 attccagcgt ggtggaagcg gggatcggcg gtccggtggg gacctggtcg gagcgcgaca
     1861 gcctgaccgc ctcgggggcc ctcggcgcgc aggcactcgg ggcgcgtgag gatctcgacg
     1921 gactgacccg gcgcgccaac atcctggtgc gctccatcaa cgaagcctac gactcgctct
     1981 ccacccatca ggcccggatg gccgccacgc cgtccatcat gccgaccaag gggtggctca
     2041 cgtcggcgtt cgcggccgag cgcgt
//
LOCUS       DBNGZW010000021         1308 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_820405, whole genome shotgun sequence.
ACCESSION   DBNGZW010000021 DBNGZW010000000
VERSION     DBNGZW010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_820405"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="AC16JC_00525"
     CDS             complement(<1..527)
                     /locus_tag="AC16JC_00525"
                     /inference="COORDINATES: protein motif:HMM:NF018184.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIR5163208.1"
                     /translation="MKLDLYARHKAEYIAPRSPAFLTIGAARYLTVAGRGDPNGPAFG
                     AAVGALYGIVYTIKMAKKAGGRDFRVSKLEGLWWGAPRGKLLIDSPVRTWRWKVMIRV
                     PTFVAPRDLLAARRTLAMLGRTLEARVRLETLREGRCVQMLHRGPYRAEHATITAMVA
                     FARGRGLRPRGRHHE"
     gene            complement(544..>1308)
                     /locus_tag="AC16JC_00530"
     CDS             complement(544..>1308)
                     /locus_tag="AC16JC_00530"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5163209.1"
                     /translation="DLPRRLLQRTAAPNAVGILEGSDPVLKHQYVVFSGHMDHIGTPP
                     DGEGCAARGADSICNGADDDGTGSVAVLELAEAFASAPIRTKRSLVFLTVSGEERGLW
                     GSAYFADHPTVPIDSIVADLNSDMVGRSDTLRDSIVAIGREHSDLGTTLDRVAGAHPE
                     LRMTPIGDRWPQEQLFFRSDHYNFARKGIPILFFTSGLHPDYHQVSDTPDRIDTEKEA
                     RFVRLEYYLGETVANAAQKPQWNPDSYQKIVQGGGH"
BASE COUNT          188 a          456 c          439 g          225 t
ORIGIN      
        1 atctcgtggt gccggccccg tgggcgcagt ccccggccgc gggcgaacgc caccatcgcg
       61 gtgatggtgg cgtgctccgc ccggtaggga ccgcggtgca gcatctgcac gcaccggccc
      121 tcgcgcagcg tttccaaccg tacccgggcc tccagggtgc ggcccagcat cgccagggtt
      181 cgccgggcag ccaggaggtc cctgggcgcg acgaatgtcg gcacccggat catcaccttc
      241 cagcgccagg tccgcacggg ggagtcgatc agcagcttgc cgcgcggagc accccaccag
      301 agcccttcca gtttcgacac cctgaaatcg cggccgccgg ccttcttggc catcttgatc
      361 gtatacacga tgccgtacaa cgccccgacg gcggcgccga acgctggtcc attggggtca
      421 ccgcgccccg cgacggtgag gtacctggcg gctccgatcg tcaagaaggc cggcgagcgg
      481 ggggcgatgt actcggcctt gtgccgagcg tagaggtcga gtttcaccac ctgcccccgg
      541 tgctcagtgc ccgcccccct ggacgatctt ctgatagctg tccggattcc actgcggctt
      601 ctgcgccgcg ttggctaccg tctcacccag gtagtactcc agccggacga accgcgcttc
      661 cttctccgtg tcgatccggt cgggcgtgtc gctgacctgg tggtaatccg ggtgcagtcc
      721 gctggtgaag aagaggatcg ggatgccctt cctcgcgaag ttgtagtgat ccgagcggaa
      781 gaagagctgt tcctgcggcc accggtcccc gatcggcgtc atgcggagct cgggatgcgc
      841 ccccgcgacg cggtcgagcg tcgtgcccag gtcggaatgc tcgcggccga tcgcgacgat
      901 cgagtcgcgg agtgtgtcgg agcggcccac catgtcgctg ttgagatccg ccacgatgct
      961 gtcgatcggc acggtggggt ggtcggcgaa atacgcgctc ccccagagtc cccgttcctc
     1021 tccgctgacg gtcagaaata cgagcgagcg tttggtgcgg atcggtgcgg aggcgaacgc
     1081 ctcggccagc tccagcaccg ccaccgagcc cgtgccgtcg tcgtcggcgc cgttgcagat
     1141 cgagtcggcg cctctggcgg cgcacccctc gccgtccggt ggcgttccga tatggtccat
     1201 gtggccgctg aacacgacgt actggtgctt cagcaccgga tcggagccct cgaggatccc
     1261 caccgcgttc ggcgcggccg tacgctgcag cagtctccgc ggcaggtc
//
LOCUS       DBNGZW010000022         2110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_615849, whole genome shotgun sequence.
ACCESSION   DBNGZW010000022 DBNGZW010000000
VERSION     DBNGZW010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_615849"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="AC16JC_00535"
     CDS             complement(<1..120)
                     /locus_tag="AC16JC_00535"
                     /inference="COORDINATES: protein motif:HMM:NF013499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HIR5163210.1"
                     /translation="MPETQRIEALGALAGQTVTVCGWVMTTRSSGKIAFVTLRD"
     gene            253..1236
                     /locus_tag="AC16JC_00540"
     CDS             253..1236
                     /locus_tag="AC16JC_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR5163211.1"
                     /translation="MAETLAAEPQPQWRASAGRSVRGAVAIGTTVVAVGTSDRAIVLL
                     DRASGQRLWRRTVRGTVAGGPLIAGSRVFAATQAVPDGRVYALDLRTGKAVWTVRTAG
                     VTAPLALSDSLVIAVTDAGDVLALEAATGDQHWKRALGRAARATPVPTADGIAIATMG
                     DSLFLLDATTGAVHAQIATPGTVLGTPACDGRRLVFGTTAGRVLAVSLPKLAVLWDRS
                     VEDAVFGAVALQSDTAVVLTGTGTLWSVPLDAPETARRVALAVPATAGPTPVVGGVLV
                     AGVEGDVLLVNAANGAVRWRMQRRAPIEEPPLVRDGQLFLVSGGGTVEVLR"
     gene            1233..1739
                     /locus_tag="AC16JC_00545"
     CDS             1233..1739
                     /locus_tag="AC16JC_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163212.1"
                     /translation="MTARILICAALVGPAAIAPCLVAQDSLAPAPRTSHNWTRPVVHY
                     GKWLSAASAVGFTLLAAHEHDTANRSWQRLLSLCRQDNAACQVGADGRYATYQAELLY
                     QETLYYDRRARHRMIVGQISVLTSAALFILDLHHGSGSPPNIPFHGLRLTAEPAGDGA
                     RMEVQIPF"
     gene            complement(1802..>2110)
                     /locus_tag="AC16JC_00550"
     CDS             complement(1802..>2110)
                     /locus_tag="AC16JC_00550"
                     /inference="COORDINATES: protein motif:HMM:NF040055.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR5163213.1"
                     /translation="ILLSDSSVPRLKAFTETTDGFRIAELDLHERGMGELAGARQSGG
                     LSLRFASFATDLDLLEAARQGAAEIIARDPALERPEHAAYKASVVARYERGFELFRVG
                     "
BASE COUNT          291 a          753 c          738 g          328 t
ORIGIN      
        1 gtcccgcagc gtcacgaacg cgatcttgcc cgaggagcgc gttgtcatca cccatccgca
       61 cacggtcacg gtctggccgg cgagcgcgcc gagcgcttcg atgcgttggg tttccggcat
      121 aggcgcgcca tactagtggc ggccgtgtgg gctgtcaacg cgatggcctg cgtgccggtc
      181 gtccgcttcg gtcccgactc ggatgcacgg ggatggccag tttacctggg ctcggcccgg
      241 cacgatgtct cggtggccga gaccctcgcg gccgagccgc aaccacaatg gcgtgccagc
      301 gccggccggt cggtgcgcgg cgccgttgcg atcggcacga ccgtggtggc ggtcggaacc
      361 tccgaccgcg ccatcgtgct gctcgaccgc gcgtcgggcc agcggctgtg gcggcgcacc
      421 gtccggggca ccgtcgcggg cggtccgttg atcgcgggtt cgcgggtgtt cgccgccacg
      481 caggcggtgc cggacggccg cgtgtacgcg ctcgatctgc ggaccggcaa ggcggtgtgg
      541 accgttcgga cggccggcgt cacggcgccg ctcgcgctct ccgattccct ggtcatcgcc
      601 gtcacggacg cgggagacgt cctggccctc gaggccgcga cgggcgacca gcactggaag
      661 cgcgcgctcg ggcgggcggc acgtgccacg ccggtgccga cggcggacgg tatcgccatc
      721 gccacgatgg gcgactccct gttcctgctc gacgcgacga ccggcgccgt ccatgcccag
      781 atcgcgacgc ccggcacggt cctcggcact ccggcctgcg acggtcggcg gctggtgttc
      841 gggaccaccg ccggtcgcgt gctcgccgtg tcgctcccca agctcgccgt gctgtgggac
      901 cgctcggtgg aagacgcggt gttcggcgcc gtcgcgctgc agtcggatac cgcggtggtc
      961 ctgacgggta ccggcacgct ctggtcggtg ccgctcgacg ctccggagac cgcacgccgc
     1021 gtggcgctgg ccgttccggc caccgccggc ccgacaccgg tcgtaggcgg cgtgctggtc
     1081 gccggggtcg aaggggacgt gctgctcgta aatgccgcga acggcgccgt ccgctggcgc
     1141 atgcaacgcc gcgcgccgat cgaggagccg ccactggtgc gcgacggtca gttgtttctc
     1201 gtctcgggag gcggaacggt ggaggtgctg cggtgacggc ccgcatcctc atctgtgcgg
     1261 cgctcgtggg cccagcggcg attgcgccct gtctcgtcgc gcaggattcg ctcgctcccg
     1321 cgccgcgcac gtcgcacaac tggacgcgac ccgtcgtcca ctacgggaag tggctcagcg
     1381 ccgcgtcggc ggtggggttc accctcctgg ccgcgcacga gcacgacacg gcgaaccgca
     1441 gctggcagcg gctgctcagc ctgtgccgac aggacaacgc cgcctgtcaa gtgggcgccg
     1501 acgggcgcta cgcgacatac caggccgagc tgctctatca ggagaccctc tactacgacc
     1561 gccgcgcccg gcaccgcatg atcgtcgggc agatctcggt gctcacttcc gccgcgctgt
     1621 tcatcctgga cctgcaccac ggcagcggca gtccgccgaa tattcccttc catggcctgc
     1681 ggctgacggc tgagccggcg ggagatgggg cgagaatgga agtgcagata ccattctaga
     1741 ggcgctatca gctatcggcc gtcagccaag ccgatagctg atagccgata gctgatagcc
     1801 atcatcccac acgaaacaac tcaaaccctc tctcataccg cgccaccacg ctcgccttgt
     1861 aagcggcgtg ctccggccgc tcgagcgcgg gatcccgggc gatgatctcc gcggcaccct
     1921 gccgcgccgc ttcgagcaga tcgagatcgg tggcgaaact cgcgaagcgc aggctcaggc
     1981 cgcccgactg ccgggccccc gcgagctcac ccatgccccg ctcgtgcagg tcgagctcgg
     2041 cgatccggaa tccgtccgtc gtctccgtaa acgccttgag tcgaggtacg ctggagtcgg
     2101 aaaggagaat
//
LOCUS       DBNGZW010000023         1919 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_2506, whole genome shotgun sequence.
ACCESSION   DBNGZW010000023 DBNGZW010000000
VERSION     DBNGZW010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1919)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1919)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1919
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_2506"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..315)
                     /locus_tag="AC16JC_00555"
     CDS             complement(<1..315)
                     /locus_tag="AC16JC_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163214.1"
                     /translation="MRALLSVANRDGIADMARELAALDVELHASPDTLAALAEAGVKA
                     QPADPAELSADSFDIVVANVEPFAPQVGGRAVPLDEAIAMIDVRTTALMSAAARSFAT
                     VAV"
     gene            411..851
                     /locus_tag="AC16JC_00560"
     CDS             411..851
                     /locus_tag="AC16JC_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163215.1"
                     /translation="MHLPVNQYLVPGVQFNAQNFPDIYVPIWVAAAVLLLLTVVLYNV
                     RVRQLHRHEPLRAMQEWLLWTGLITFGLILVGTIFQFYFLFILTFIVVGIGAFVWIRF
                     FHFPPYIAAYNEQLRRARFFSQSKYKAAESTVRSRRTRGARRRR"
     gene            882..1340
                     /locus_tag="AC16JC_00565"
     CDS             882..1340
                     /locus_tag="AC16JC_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012417.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5163216.1"
                     /translation="MEIRRFGPGHRRAEGPPGTKGVAGALLMSDQRGVVTEVAFGPYA
                     MITPHANETNTALFIVISGGGFVQVGEERARVNHGEAVLWPPGIPHGAYTDGTEMRAI
                     IVELAGSEEHRVVDSTAAESTKAPAERATGRLADRPRTRDEYDSSEGEPW"
     gene            1367..>1919
                     /locus_tag="AC16JC_00570"
     CDS             1367..>1919
                     /locus_tag="AC16JC_00570"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007873843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5163217.1"
                     /translation="MPLTTERFAGLAELFNEGGDPKWCWCQYFRKQGLDWTNSTTAGN
                     RSALQQLAARPDLAPGLVGYRDGRAVGWVSLAPREDYARLENSKVLARVDATPVWSIV
                     CFVVSKRARGAGVAAAMLQGAIAYAREHGATMLEAYPVAATRGRITASSAFHGVQSMF
                     EKAGFAVVETRQWNKTSPLRPIMR"
BASE COUNT          300 a          669 c          645 g          305 t
ORIGIN      
        1 aacggcgacc gtggcgaagc tgcgcgccgc agccgacatc aacgccgtcg tgcgcacatc
       61 gatcatggcg atcgcctcgt caagcggcac ggcacgcccg cccacctggg gcgcgaaggg
      121 ctcgacgttg gcgacgacga tgtcgaacga atcggccgat agctccgccg gatcagccgg
      181 ctgcgccttg acgcctgctt ctgccagggc ggcgagcgtg tccggtgagg cgtggagctc
      241 gacatctagg gctgccagtt cgcgcgccat atcggcgatg ccatcgcgat tggcgaccga
      301 gagcagggcc cgcacgtctc ctcctgtccc tctcggtggg cgcccgcggc tccatgggct
      361 cagagcgcgg cagggcgggc cgagtataga cgaggcgcta tcctgcggcc gtgcacctgc
      421 ctgtcaacca gtacctggtc cccggggtcc agttcaacgc ccagaacttc cctgatatct
      481 acgtcccgat ctgggtcgcg gcggcagtcc tgctcctgct gacggtcgtc ctgtacaacg
      541 tccgtgtccg ccagctgcac cgccacgagc cgctccgagc gatgcaggag tggctgctct
      601 ggaccggcct catcaccttt gggctgatcc tcgtgggcac gattttccag ttctacttcc
      661 tgttcatcct gacctttatc gtcgtcggca tcggagcctt cgtgtggatc cgcttctttc
      721 acttcccgcc gtatatcgcg gcgtacaacg agcagctccg ccgagcacgg ttcttcagcc
      781 agagcaagta caaggcggcc gagtcgacag ttcgctcgcg gcgaacacgc ggcgcgcgac
      841 ggcgccgctg atccccggcg ccgggccgga caggtagcgc gatggagatc cgtcgcttcg
      901 ggcccggcca tcgccgcgcg gagggaccgc cgggtaccaa gggagtcgcc ggcgcgctgc
      961 tcatgagcga ccagcgcgga gtggtgacgg aagtcgcctt cggtccgtac gcgatgatca
     1021 cgccgcacgc caacgagacc aacaccgcac tgttcatcgt catcagcggc ggtggcttcg
     1081 tgcaggtggg cgaagagcgg gcgcgcgtca accacggcga ggcggtgctc tggccgccgg
     1141 gcatccccca cggcgcctat accgacggga cggagatgcg cgcgatcatc gtcgagctgg
     1201 ccggcagcga ggagcaccgg gtcgtcgact ccactgcggc tgagtccacc aaggcgccgg
     1261 ccgagcgggc aacgggccgc ctcgccgacc ggccgcgcac gcgcgacgaa tacgactcat
     1321 cggagggcga gccctggtaa gcgcagccgg cacgaatgtc gaggtagtgc cgctgaccac
     1381 tgaacggttc gctggcctgg ccgagctgtt caatgagggt ggcgatccca agtggtgctg
     1441 gtgccagtac ttccgcaagc agggcctcga ctggaccaac tcgaccaccg ccggcaaccg
     1501 ctccgccctg cagcaactgg ccgcgcggcc cgacctcgcg ccgggactcg tcggctaccg
     1561 cgatggccgc gccgtgggct gggtgagcct cgccccgcgc gaggactacg ctcggctcga
     1621 gaactcgaaa gtgctcgccc gagtcgacgc gacgccagtc tggtcgatcg tctgtttcgt
     1681 ggtctcgaaa cgggcgcgcg gcgcgggtgt cgcggcggcc atgctccagg gtgcaatcgc
     1741 gtacgctcgg gagcacggcg cgaccatgct cgaggcttac ccggtggccg cgacgcgcgg
     1801 ccgtatcacc gcctcgtccg cgttccacgg cgttcaatcc atgttcgaaa aggccggctt
     1861 cgccgtcgtc gagacgcgcc aatggaacaa gacgtcgccg ctccgtccga tcatgcggc
//
LOCUS       DBNGZW010000024         5573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1571008, whole genome shotgun sequence.
ACCESSION   DBNGZW010000024 DBNGZW010000000
VERSION     DBNGZW010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5573
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1571008"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /gene="uvrB"
                     /locus_tag="AC16JC_00575"
     CDS             complement(<1..1462)
                     /gene="uvrB"
                     /locus_tag="AC16JC_00575"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HIR5163218.1"
                     /translation="MPFDLVSPFSPAGDQPEAIRSLSAGLVRGDRYQTLLGVTGSGKT
                     MTMAHVIAAHGRPTLVLSHNKTLAAQLYGELKTFFPRNAVEFFISYYDYYQPEAYVPQ
                     TDTYIAKDSSINDDIDALRLRATSSLLERDDVVIVASVSAIYGLGDPEAYRRLLLTVE
                     TGEDRGRDGILEDLVRINYQRNDVAFERGTFRVRGDTIEIFPAYDEQGVRIELWGDAV
                     ERITKIDPTTGNAIAQLNRAVVYPATHFVTERPTVERAVAAIRAELKERLAELRAQNK
                     LVEAQRLESRTLFDVDMLLEVGRCSGIENYSRHLTARQPGERPAVLLDYFPDDFLCVI
                     DESHQTLPQLGGMYEGDRSRKQTLVDYGFRLPSALDNRPLRFDEFMKLVPRMVFVSAT
                     PGDQELRLSEGIVVEQIIRPTGLVDPEVEIRPVKGQVDDLLHEIRERARATERVLVTT
                     LTKRMAEDLTDYLQQHGVRVRYLHSEIDAIERVEIIR"
     gene            complement(1505..3688)
                     /locus_tag="AC16JC_00580"
     CDS             complement(1505..3688)
                     /locus_tag="AC16JC_00580"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008728 - GTP diphosphokinase activity
                     [Evidence IEA];
                     GO_function: GO:0008893 - guanosine-3',5'-bis(diphosphate)
                     3'-diphosphatase activity [Evidence IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HIR5163219.1"
                     /translation="MTTAASSTPSLAPELAKALAPLRERLDLDLLSRAYRTSARAHSG
                     QKRKSGEDFLSHSVAVATILAEQQMDSTTIAAALLHDVVEDSDVSLEQIRKEFGAEVA
                     DLVDGLTKLSGLTFRSAEEAAAENYRKLLLSIAKDARVIIIKLADRLHNMRTLEHLSE
                     DKQRRIALETQEIYAPLAHRFGMAGVKAELEDLAFRFLEPEEYRALLKKIQARRAERD
                     QTIERLRAPLAEELKRAGLTDFEITGRPKNLWSIDKKMKRRDKPFEEIYDLLAMRVLV
                     NSVADCYHALGVIHHQWTPVQERIKDYIASPKSNGYQSLHTTVFGPGGQLYEIQIRTR
                     DMHRTAEYGIAAHWLFKEGKAPDELDRHLAWFRQLLELQQDAHSPEEFLEFLKVDLYQ
                     DEIFVFTPRGDVKRLPKDATPIDFAFAVHTEVGYRCHGARVNGRIAPLHRPLKNGDSV
                     EVLTSPQARPSRDWLAHVHTGRARHKIRQWIRSEEHTRSVALGKEILDREVRRRRLEP
                     PDADQLARAAATLSVGNAEQLEAVLGRGEVALGQVMRALYPDLPAEDLQAPKPTAFGR
                     VIQRLRLGRGIRIQGVDGLMVRYAQCCQPVPGDPVVGYVTQGRGISIHRSDCPNLLPL
                     SAEAERRVEIDWQESEGETFVVRLVIAGEDRRGLYADICEAVSQSGTNIRHAELSSRD
                     SAVYGYLLVEVENHTHLNKVLRAVRRVKGVTEIARRDVGPHQAQT"
     gene            complement(3715..4401)
                     /gene="radC"
                     /locus_tag="AC16JC_00585"
     CDS             complement(3715..4401)
                     /gene="radC"
                     /locus_tag="AC16JC_00585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009440817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HIR5163220.1"
                     /translation="MAAVASSTAGAPDRPRERLWRLGSHALTVQELLAILLGTGCAGR
                     SALEVAAALLARVDSSIVRLASRPAADVARIPGVGRAKAARVAAALELGRRLGAELTP
                     PPERIAGPADVHRRCAPRLRDLAVEEFHVLALGSQSQVLADLLITRGILNSSLVHPRE
                     VFRGAIAEAAAGIIVVHNHPSGDPTPSADDRAVTRQLVDAGRLLDLPVYDHVIIGAER
                     YVSFAEAGLL"
     gene            complement(4477..>5573)
                     /locus_tag="AC16JC_00590"
     CDS             complement(4477..>5573)
                     /locus_tag="AC16JC_00590"
                     /inference="COORDINATES: protein motif:HMM:NF019155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HIR5163221.1"
                     /translation="DRIARWMDRGGAEEGEVITHPWVTGAIGQAQKRVELQNFQARKR
                     LLEYDDVMNQQREVMYSLRLFALEGGEELKAEALRMIDAAVERLVDERTAVYKDPAEW
                     DRELLTTEMLQRYLLSVPDLTDPAKVRGRDDLVRAAQQAGHESFEGKLSQLRDADAML
                     RRDLEARLRRETDPVVRAAIEARLAEQTGLAEQALSQIMLSVIDEKWKDHLYDLDQLK
                     AGIQYRGWGQKDPLIEYKQDAFEMFKGLIDDVRSTFAERWLKLQIQIGPPGPGSTGPA
                     GGRPGPSGGPGSGLLGGPKPRRPPPMVAMKPEADGLVSGGTSTGPLPPSGRPTPVEAA
                     VANPYAGVGRNDPCPCGSGKKFKKCHGAAS"
BASE COUNT          817 a         1887 c         1957 g          912 t
ORIGIN      
        1 cgcggatgat ctccacgcgc tcgatcgcgt cgatctccga gtgcaggtag cgcacacgca
       61 cgccgtgctg ctggagatag tcggtcagat cctcggccat ccgcttggtg agcgtcgtga
      121 cgagcacgcg ctcggtggcg cgggcgcgct cccggatctc gtgcagcagg tcgtccacct
      181 gccccttcac cggccggatt tcgacctcgg ggtccacgag ccccgtggga cggatgatct
      241 gctcgactac gatgccctcg gagagccgca gctcctggtc acccggcgtg gcggacacga
      301 acaccatccg cggtaccagc ttcatgaact cgtcgaagcg cagcggacgg ttgtcgagcg
      361 cggagggcag gcggaacccg tagtccacca gggtctgctt gcgcgagcga tccccttcgt
      421 acatcccgcc gagctgcggc agcgtctggt gcgattcgtc gatgacacag aggaaatcgt
      481 cggggaaata gtcgagcagc accgccggtc gttcgccggg ctggcgcgcc gtgaggtggc
      541 gcgagtagtt ctcgatgccg ctgcagcgcc ccacctcgag cagcatgtcg acatcgaaca
      601 gcgtccggga ctccagccgt tgcgcctcga cgagcttgtt ctgcgcccgc agctccgcca
      661 gccgctcctt gagctcggcc cggatggccg cgaccgcgcg ctcgaccgtc ggccgctcgg
      721 tcacgaagtg cgtggccggg tagacgacgg cgcgattgag ctgggcgatc gcgttgccgg
      781 tggtcgggtc gatcttggtg atgcgctcga ccgcgtcgcc ccacagctcg atgcggacgc
      841 cctgctcgtc gtaggccggg aagatctcga tggtgtcgcc ccggacccga aaggtgccgc
      901 gctcgaaggc gacgtcgttg cgctgatagt tgatccggac gagatcctcg agaatcccgt
      961 cgcgtccccg gtcctccccg gtttcgacgg tgagcagcag ccggcggtac gcctccggat
     1021 cgcccaggcc gtagatcgcc gagacgctcg ccacgatgac gacatcgtcg cgctccagca
     1081 ggctcgaggt cgcccgcaga cggagtgcgt cgatgtcgtc gttgatggac gaatccttgg
     1141 cgatgtaggt gtcggtctgc gggacgtagg cttcgggctg atagtagtcg tagtacgaaa
     1201 tgaagaattc caccgcgttg cgggggaaga acgtcttgag ctctccgtac agctgcgcgg
     1261 ccagggtctt gttgtgggag aggacgagcg taggacggcc atgggcggcg atgacgtggg
     1321 ccatcgtcat cgtcttgccc gacccggtga ctccgagcag ggtctgatag cggtccccgc
     1381 gcaccagtcc ggcggacagg gaccggatgg cctcgggctg gtcgcccgcc gggctaaacg
     1441 gcgatacgag atcgaacggc atggcaggaa gctaacgacc gcgccaaggc cggcaggagt
     1501 gcggtcaggt ctgggcctgg tggggaccga cgtcccggcg cgcgatttcg gtcacgcctt
     1561 tcacccggcg cacggcgcgc aggaccttgt tcaggtgcgt gtggttctcc acctcgacga
     1621 gcagatagcc gtacacggcg ctgtcgcggg aggagagctc cgcgtggcgg atgttggtcc
     1681 ccgactggct cacggcctcg cagatgtccg cgtagagacc gcgccggtcc tcgcccgcaa
     1741 tcacgagccg caccacgaac gtctcacctt cggactcctg ccaatcgatc tcgacgcggc
     1801 gttcggcctc ggccgacagc gggagcaggt tgggacagtc gctgcggtgg atggagatcc
     1861 cccgcccctg ggtgacgtag cccaccaccg gatcgccggg tacgggttgg cagcactgcg
     1921 cgtagcggac catgaggcca tccacgccct ggatccggat gccgcggccg agacgcaggc
     1981 gctggatgac gcgaccgaac gccgtcggct tgggcgcctg caggtcctcg gccgggagat
     2041 cggggtagag cgcccgcatc acctgcccaa gcgccacttc accccgcccc agcacggctt
     2101 cgagctgctc ggcgtttccc accgagagcg tggcggccgc ccgcgcgagc tggtccgcat
     2161 cgggcggctc caggcgccgg cgccggacct cgcggtccag gatttccttg cccagcgcga
     2221 cgctgcgggt gtgctcctcg gaccggatcc actgacggat cttgtgccgc gcgcgaccgg
     2281 tgtgtacgtg cgccagccag tcgcgtgagg ggcgggcctg cggactcgtc agcacctcga
     2341 cggagtcgcc gttcttcagc gggcgatgca gcggcgcgat gcggccgttc acccgggcgc
     2401 cgtgacagcg gtagccgacc tcggtgtgca ccgcgaacgc gaagtcgatg ggcgtcgcgt
     2461 ccttgggcag ccgtttcacg tcgccgcgcg gcgtgaacac gaagatctcg tcctggtaga
     2521 ggtcgacctt cagaaactcg aggaactcct ccggcgaatg cgcatcctgc tgcagctcga
     2581 gcagctgccg gaaccaggcg aggtggcggt cgagctcatc cggggccttc ccctccttga
     2641 acagccagtg cgcggcgatg ccgtactcgg cggtgcggtg catgtcgcgc gtgcggatct
     2701 ggatctcgta gagctgcccg ccgggaccga acacggtggt gtggagcgac tgatagccgt
     2761 tggacttcgg gctcgcgatg tagtccttga tccgctcctg cacgggcgtc cactggtggt
     2821 ggatgacccc gagcgcgtgg tagcagtcgg cgaccgagtt gaccagcacc cgcatcgcca
     2881 gcagatcgta gatctcctcg aacggcttgt cccgccgctt catcttcttg tcgatggacc
     2941 agaggttctt cggccgcccg gtgatctcga agtcggtgag gccggcgcgc ttcagctcct
     3001 cggccagcgg cgcccgcagc cgctcgatcg tctggtcgcg ctccgcgcgg cgcgcctgga
     3061 tcttcttgag cagcgcccgg tactcctcgg gctcgaggaa gcgaaaggcg aggtcttcga
     3121 gctccgcctt cactcccgcc atgccgaagc ggtgggccag cggcgcgtag atctcctgcg
     3181 tttccagcgc aatgcgacgc tgcttgtcct cggagaggtg ctcgagcgtc cgcatgttgt
     3241 gcagccggtc ggccagcttg atgatgatga cccgggcatc cttcgcgatg gagaggagca
     3301 gcttgcggta gttctccgcc gccgcctcct cggcggagcg gaacgtcagc cccgacagct
     3361 tggtcaaccc gtccaccagg tcggcgacct cggccccgaa ctccttgcgg atctgctcga
     3421 gggagacgtc ggagtcctcc accacgtcgt gcaacagggc cgcggcgatg gtggtcgagt
     3481 ccatctgctg ctcggccaga atggtggcca ccgccacgct gtgcgacagg aaatcctcgc
     3541 cggacttgcg cttctggccg gaatgggccc gggcgctggt gcggtaggca cggctcagga
     3601 ggtccaggtc gaggcgctcg cgcaacggcg ccagcgcctt ggccagctcc ggggccaggg
     3661 acggggtgga cgatgctgcc gtagtcaccc gtggaggatg gcgaaccggc gggtttagag
     3721 caagccggct tccgcgaagc tcacgtagcg ctcggcaccg atgataacgt ggtcgtatac
     3781 cggaagatcc agcaatcgcc cggcatccac gagctggcgc gtgacggcgc ggtcgtcggc
     3841 cgagggggtg ggatcgccac tggggtggtt gtggaccacg atgatcccgg ctgcggcctc
     3901 ggcgatggcg ccccggaaca cctcccgcgg gtgcaccagg gagctgttga ggatcccgcg
     3961 cgtgatgagg aggtcggcga ggacctggct ctggctgccg agcgccagca cgtggaattc
     4021 ctcgacggcg agatcgcgga gccggggcgc gcagcgccga tggacgtccg cgggtccggc
     4081 gattcgctcc ggaggcggcg tcagctcggc gcccagccgg cgtcccagtt ccagtgcggc
     4141 cgcgacccgc gccgccttgg cgcgcccgac cccggggatc cgggccacgt ccgcggccgg
     4201 ccggctggcg agccgcacga tggaactgtc gacccgggcg agcagggcgg cggcgacctc
     4261 gagggcgctg cgaccggcgc atccggttcc caggagaatg gcgaggagct cctggacggt
     4321 gagcgcgtga ctgccgaggc gccagagccg ctcgcggggt cggtcgggcg cgcctgcggt
     4381 ggacgaggcg actgcggcca tgcgatcaac gtacggcggg gaggcgccgc ccgcggtatc
     4441 aaagaaacgc gggcgacgcc ttttgcgtgc cggcgctcac gacgccgcgc cgtggcactt
     4501 cttgaacttc ttgccgctgc cgcagggaca ggggtcgttg cgtccgacgc cggcgtacgg
     4561 attcgccacc gccgcctcga ccggagtcgg tcggccagac ggaggcaacg ggccggttga
     4621 agtgccgcca ctcacgagcc cgtcggcttc cggcttcatc gccaccatgg gaggtgggcg
     4681 gcgcggcttg ggcccgccga ggagtcccga accggggccc ccagaaggcc cggggcgacc
     4741 accagcgggc cccgtagagc ctggcccagg cggcccgatc tggatctgga gcttgagcca
     4801 gcgctcggcg aacgtgctgc gcacgtcgtc gatcaggccc ttgaacatct cgaacgcgtc
     4861 ctgcttgtat tcgatcagcg gatccttctg tccccagccg cgatactgga tgcccgcctt
     4921 gagctgatcg agatcgtaga ggtggtcctt ccacttctcg tcgatcaccg agagcatgat
     4981 ctgggacagg gcctgctcgg cgagccccgt ctgctccgcc agccgggcct cgatcgccgc
     5041 gcgcaccacc ggatcggttt cgcgccgcaa ccgcgcttcg aggtcgcgcc gcagcatcgc
     5101 atcggcgtcg cgcagctggg acagcttgcc ctcgaatgac tcgtgcccgg cctgctgggc
     5161 cgcgcgcacc aggtcgtccc ggccccggac cttcgcggga tcggtgaggt ccggcaccga
     5221 gagcagatag cgctgcaaca tctccgtggt gagcagctcg cggtcccact cggcgggatc
     5281 cttgtagacg gcggtgcgct cgtccaccag ccgctcgacc gccgcgtcga tcatccgcag
     5341 cgcttccgcc ttcagctctt ccccgccctc gagcgcgaac agccgcagcg agtacatgac
     5401 ctcacgctgc tggttcatga cgtcgtcgta ctcgagcagc cgcttccggg cctggaagtt
     5461 ctgcagctcg acccgctttt gcgcctggcc gatggcgccg gtgacccagg ggtgggtgat
     5521 cacctcgccc tcttcggcgc cgccgcggtc catccagcgc gcgatgcggt cgg
//
LOCUS       DBNGZW010000025         5254 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1638942, whole genome shotgun sequence.
ACCESSION   DBNGZW010000025 DBNGZW010000000
VERSION     DBNGZW010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5254)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5254)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5254
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1638942"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="AC16JC_00595"
     CDS             complement(<1..640)
                     /locus_tag="AC16JC_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163222.1"
                     /translation="MSDGIAAMRDVVVVGGGCYGTFYATQLARAKERGKAAFRTVVVV
                     DRDPTCRARRELPESADRRFVTSEWDAFFDRHLAAARPTAAGDPPDYVVPAPHMSHLM
                     FEWLVRRTRARCPDRSVDVVPVPDSLGTPYDRTGPDHTRYVSFADWICPTHCIEPVVC
                     PAIGGPRSWEMAAAVQGLAERLRASGASITGPVLFICRHHVYGVGTFAVDAVL"
     gene            complement(637..1338)
                     /locus_tag="AC16JC_00600"
     CDS             complement(637..1338)
                     /locus_tag="AC16JC_00600"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5163223.1"
                     /translation="MTGAGRRLGAAIAAGLARAGYDIAAHHHASADGAEATLGAVRAA
                     GRRGERFAADLRDPAAARALPGRVAETFGRLDVLVNCAAVMERREFAEITPDDWNDAL
                     ALNLSAALFTAQGAAPHLRRVRGKIVNLADVAAFAVWPAYLPLNVSKAGVVMLTRGLA
                     RVLAPEVTVNAVAPGAVLPPEDWPAEARDHLAATTPLRRLGSPEDVVRAVLFLLDSDY
                     ITGVVLPVDGGRLVR"
     gene            complement(1369..2097)
                     /locus_tag="AC16JC_00605"
     CDS             complement(1369..2097)
                     /locus_tag="AC16JC_00605"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5163224.1"
                     /translation="MTGELDGRVALITGVGRAGQIGHAVATGFGSAGARLVLADRSAE
                     ALDERVREFAAARIEAAAAPGDLTEPHAARAAVGLAEARFGGLDIVVNVAGGLTTYGP
                     FEGIDVTAFEREIAINLRTVFCVCQAAVPSLRRRGGGAIINFASIAVLHPQPNLAAYL
                     AAKGGVAALTRALARALRDDGAGIRVNALAPEAVRTSTNVQDMGSDAKLVEMADVVRT
                     VLWLASDAARAVTGQILPLLPGSH"
     gene            complement(2087..2386)
                     /locus_tag="AC16JC_00610"
                     /pseudo
     CDS             complement(2087..2386)
                     /locus_tag="AC16JC_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012787.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
     gene            complement(2402..2545)
                     /locus_tag="AC16JC_00615"
     CDS             complement(2402..2545)
                     /locus_tag="AC16JC_00615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR5163225.1"
                     /translation="MSDVLPVGGKAPDFQAQASDGNTYHLAELLRRTHVALVFYPGNN
                     TPG"
     gene            complement(2562..3425)
                     /locus_tag="AC16JC_00620"
     CDS             complement(2562..3425)
                     /locus_tag="AC16JC_00620"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR5163226.1"
                     /translation="MPGPRRRAPTAHVDLHCHSTASDGQTSPEVVVQRAAAVGLSALA
                     LTDHDTLDGMPGAVEAGHALGVRIVSGCEFSVQAPWGELHLLGYFLPAGDAALDQFLA
                     RMREARERRGTRMIEKLQHLGVEIDLEDLAFQAGGGAVGRPHVARALIDAGVVDGLEE
                     AFDRFLGRGRPAFVEKELPPLREVADLVHTGGGLVLAAHMGERAHEEQLRTFRQDGLD
                     GIEVRHPSHSPRVERRLTKLARQLDLAISGGSDWHGESSAGISHAPLGGMQVPLEWLE
                     ELECRKAKAYG"
     gene            complement(3385..3654)
                     /locus_tag="AC16JC_00625"
     CDS             complement(3385..3654)
                     /locus_tag="AC16JC_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163227.1"
                     /translation="MARRELTVDGQRWEVFPSGRPTVYGQDQYGLVFQLGTGTERKRR
                     FTRYAPTGVRRTDASLSELSERTLLELFHQSQPAWTSPESAYGAR"
     gene            complement(3657..3971)
                     /locus_tag="AC16JC_00630"
     CDS             complement(3657..3971)
                     /locus_tag="AC16JC_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HIR5163228.1"
                     /translation="MPKVLTAARVRVPAANEIDYLATLRELAQFADARGQRIWVFRNA
                     KDPRLFIEFSESMTEMSHRAQASRLPEETKLEKRLQSLVTYSPDAWELWSEVSLAAPA
                     ET"
     gene            complement(3974..>5254)
                     /locus_tag="AC16JC_00635"
     CDS             complement(3974..>5254)
                     /locus_tag="AC16JC_00635"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR5163229.1"
                     /translation="LGDTVKRDEPLFEISTDKVDAEIPAPTAGTLVEIKVQEGQTVPV
                     QTLVAVLDTEQGAVVASSAPAAAPAVPAAPKAAPVPASTPVAVSAPPVPRPSSPVPPA
                     SPVPPKGDGQETAEERLRRRSTPLVRKIATEHQLDISQIPGTGFAGRVTKQDILGFIE
                     HAPAAAPAAAAPTAPRAPLSAGAEVVHPGAEPWPGDRVEPWSKIRKITADHMIMSRRV
                     SAHVASFFEIDYTRVAQLRAKFKAAYAERGVSLTYLAFIAKACAANLRKHPIVNAAVS
                     SDSTIYRQDVNIGIAVALDWGLIVPVVKHADELSLLGLARAINDLGERARTKKLSPDD
                     IQRGTFTITNPGGFGPYAGIPIINQPQVAILGVGTIEKRPKVVTQDGTDAIAIRTMGM
                     LTMSYDHRVVDGADADKFLADVKATLQDFPEQEM"
BASE COUNT          758 a         1873 c         1866 g          757 t
ORIGIN      
        1 ccagcaccgc gtccacggcg aacgtcccca cgccgtacac gtggtggcgg cagatgaaca
       61 ggacgggccc ggtaatggac gcgccgctcg cccgcaaccg ttcggccagg ccctgaacag
      121 ccgccgccat ctcccacgac cggggcccgc cgatcgccgg gcagacaacg ggctcgatgc
      181 agtgtgtcgg gcagatccaa tcggcgaagg agacgtaacg cgtgtggtcg gggccggtac
      241 gatcgtaggg tgtcccgagc gagtccggga ccgggacgac gtccaccgag cgatccggac
      301 agcgcgcccg cgtgcggcgc accagccatt cgaacatgag gtgcgacatg tgcggggcgg
      361 gtacgacgta gtcgggcgga tccccggccg cggtgggtcg cgccgccgcg aggtgccggt
      421 cgaagaaggc gtcccactcg ctcgtgacga atcttcgatc ggccgactcg gggagctcgc
      481 ggcgcgcgcg gcaggtcgga tcacggtcca ccacgacgac cgtccgaaac gccgctttgc
      541 cgcgctcctt cgcccgggcg agctgggtgg cgtagaaggt cccgtaacag ccgcctccca
      601 cgaccacgac gtcccgcatc gccgcgatcc catcgctcat cgcaccaggc gacccccgtc
      661 caccgggagc accacccccg tgatgtagtc gctgtccagc aagaacagga cggcccgcac
      721 cacgtcctcg gggctgccga gccggcgcag cggcgtagtc gcggcgaggt ggtcccgcgc
      781 ctcggcgggc caatcctcgg ggggaagcac ggccccgggc gcgacggcat tcacggtcac
      841 ttccggagcc agcacgcgcg cgagcccgcg tgtcagcatg acgaccccgg ccttgctcac
      901 gttcagcggg aggtacgcgg gccacaccgc gaaggcggcc acgtcggcga ggttgacgat
      961 cttgccgcgc acgcgccgca ggtgcggcgc ggctccctgg gcggtgaaca gcgccgccga
     1021 gaggttgagc gccagcgcgt cgttccagtc gtccggggtg atctccgcga actcgcgtcg
     1081 ctccatgacg gccgcgcaat tgacgagcac gtcgagccgg ccgaacgtct cggcgacacg
     1141 cccgggcagc gcgcgcgcgg ccgcgggatc acggaggtcc gcggcgaacc gctcgccccg
     1201 gcgcccggcc gcgcgaacgg cgccgagggt cgcttccgct ccgtccgcgg acgcgtggtg
     1261 atgtgcggcg atgtcgtatc ccgcccgagc gagccccgcg gcgatcgcgg cgccgaggcg
     1321 acggccggcg ccggtcacga gcgcgactcg cccggtgggc tccaccgatc agtgcgaccc
     1381 ggggaggagg gggaggatct gccccgtcac cgcgcgggcg gcgtcggacg cgagccagag
     1441 cacggtacgc accacgtcgg ccatctccac cagtttcgca tccgacccca tgtcctgcac
     1501 gttggtggag gtgcggaccg cctccggggc gagcgcgttc acgcggatgc cggccccgtc
     1561 gtcccgcagc gcgcgggcga gcgcgcgggt cagcgccgcg acgccgccct tggcggccag
     1621 gtacgcggcc aggttgggct gggggtgcag caccgcgatg gacgcgaagt tgatgatcgc
     1681 cccgccgccg cggcgccgca gcgacgggac ggcggcctga cacacgcaga aaacggtacg
     1741 gagattgatc gcgatttcgc gctcgaaggc cgtgacgtct atcccctcga atggcccgta
     1801 cgtggtgagg ccaccggcga cgttgaccac gatgtcgagg ccgccgaaac gggcctcggc
     1861 gagccccacc gcggcgcgcg ccgcgtgcgg ctcggtcagg tcccccgggg cggcggcggc
     1921 ttcgatgcgg gcagccgcga actcccggac ccgctcatcc agggcttcgg cggagcggtc
     1981 ggcgaggacg aggcgggcgc ccgcggatcc gaagcccgtc gccacggcat ggccgatctg
     2041 ccctgcgcgc cccaccccgg tgatgagcgc gacccgcccg tcgagctcac ccgtcacggc
     2101 cgcccgactc caccgcggcg accacctcgt ccggaggcgg tgcgccgcgc tgggcgtacc
     2161 ggacggtgcc gtcccgcccc acgacgtaca ccgtgcgggc gatccccgtc ccgtcgtcct
     2221 tgagcgcgtg atacgcccgt gcgatggctc gatcgggatc ggacagcagc gggaagttga
     2281 atttcatctt ctccacgtac ttccggtggc tgtcgagact cgccggattc acgcccagag
     2341 gctggatccc ggctgcttcg aatctgctca tctcatcgcg cacggcggag agttggcggt
     2401 ttcacccggg cgtgttgttt cccggataga acaccagcgc cacgtgggtg cgtcggagca
     2461 attcggccag gtggtaggtg ttcccgtccg acgcctgcgc ctggaagtcc ggagccttgc
     2521 cgccgaccgg tagtacgtcg ctcatgaagc gtgtctccct ttcatccgta tgctttcgcc
     2581 ttgcgacact ccagctcctc cagccactcc agcggcacct gcatcccgcc caacggcgcg
     2641 tgggagatcc cggcgctcga ctccccgtgc caatccgagc cgccggagat cgccagatcg
     2701 agctggcgtg ccagcttggt gagccgccgt tccacccgcg gcgagtggct cgggtgacgc
     2761 acctcgatgc cgtccagccc gtcctgccgg aatgtgcgca gctgctcctc gtgagcgcgc
     2821 tcccccatgt gcgcggcgag caccagcccg cctcctgtgt gtaccaggtc cgccacctcg
     2881 cgcaggggcg gcagctcctt ctccacgaag gccgggcggc cgcggccgag aaagcgatcg
     2941 aaggcttcct ccagaccgtc caccacgccc gcgtcgatca gcgcgcgggc cacgtggggc
     3001 cgtccgaccg cgccgccgcc ggcttggaac gccaggtcct cgagatcgat ctcgacgccc
     3061 agatgctgca gcttctcgat catgcgggtg ccgcgccgct cccgcgcctc gcgcatccgg
     3121 gcgaggaact ggtcgagcgc ggcgtcgcct gcaggcagga agtacccgag caggtgcagc
     3181 tcgccccacg gggcctggac gctgaattcg cacccggaca cgatgcgcac acccagcgca
     3241 tggcccgcct ccacggcgcc tggcatgccg tcgagcgtat cgtgatcggt gagcgccagc
     3301 gcggacagcc cgacggcagc cgcccgttgc accacgacct cggggctcgt ctggccgtcc
     3361 gaggcggtgg aatggcaatg caggtcaacg tgcgccgtag gcgctctccg gcgaggtcca
     3421 ggcaggttgg gattggtgga acagctcgag cagcgtccgc tcgctcagct cgctcagcga
     3481 cgcgtcggtc cgccgcaccc cggtgggggc gtaccgcgtg aaccggcgct tgcgctcggt
     3541 gccggtgccg agctggaaca ccaggccgta ctggtcctga ccgtacacgg tggggcggcc
     3601 ggacgggaac acctcccagc gctggccatc cacggtgagc tcgcggcggg ccacgctcag
     3661 gtctccgccg gggccgcgag cgagacctcg ctccacagct cccacgcgtc gggggagtag
     3721 gtcacgaggc tctgcagccg cttctcgagc ttcgtttcct cgggcaggcg ggacgcctgc
     3781 gcacgatggg acatctccgt catgctctcc gaaaactcga tgaacaggcg cgggtccttg
     3841 gcgttgcgga acacccagat gcgctgcccc cgggcgtcgg cgaactgggc cagctcgcgc
     3901 agcgtcgcca gatagtcgat ctcgttggcg gcgggcacac ggacccgggc tgcggtgagg
     3961 accttcggca tggctacatc tcctgctcgg ggaagtcctg cagcgtggcc ttcacgtcgg
     4021 ccaggaactt gtcggcgtcc gcgccgtcca ccacccggtg gtcgtaggac atggtgagca
     4081 tgcccatggt gcggatggcg atcgcgtccg tgccgtcctg ggtcacgacc ttgggacgct
     4141 tctcgatcgt gccgacgccg aggattgcga cctgcggctg attgatgatg gggataccgg
     4201 cgtacggacc gaacccgccg ggattcgtga tcgtgaacgt gccgcgctgg atatcgtcgg
     4261 gagagagctt cttggtgcgc gcccgctcgc ccagatcgtt gatggcacgc gccaggccga
     4321 gcagcgacag ctcgtcggca tgcttgacga cgggaacgat gagtccccag tcgagtgcta
     4381 cggcgatccc gatgttcacg tcctggcggt agatcgtgct gtcgctcgag acggcggcat
     4441 tgacgatcgg atgcttgcgc agattggccg cgcaggcctt cgcgatgaac gcgaggtagg
     4501 tgaggctcac cccgcgctcg gcataggcgg ccttgaactt cgcccgcagc tgcgccaccc
     4561 gcgtgtagtc gatctcgaag aagctggcca cgtgcgccga cacgcggcgc gacatgatca
     4621 tgtgatccgc ggtgatcttg cggatcttcg accacggctc gacccggtcg cccggccagg
     4681 gctccgcccc aggatgcacc acttccgcac cggcggagag cggggcgcgg ggcgcggtcg
     4741 gagcggcagc cgcgggtgcg gccgcgggcg cgtgctcgat gaagccgagg atgtcctgct
     4801 tcgtcacgcg gcccgcgaag ccggtccccg gtatctgcga gatgtcgagc tggtgctcgg
     4861 tcgcgatctt gcggaccagc ggcgtcgagc gccgccgcag ccgctcctcg gcggtctcct
     4921 gaccgtcacc cttgggaggg acgggggacg cgggagggac gggggacgag ggacggggga
     4981 cggggggcgc ggatacggcc acaggcgtcg acgcggggac tggcgccgct ttcggagcgg
     5041 cggggaccgc gggtgcggcg gcgggcgcgg agctggccac gaccgccccc tgctccgtgt
     5101 ccagtaccgc cacgagcgtc tgcaccggaa ccgtctggcc ttcctgcacc ttgatctcga
     5161 ccagcgtccc cgcagtcggg gccggaatct cggcatccac cttgtcggtg gagatctcga
     5221 acaacggctc gtcccgcttc acggtgtcgc cgag
//
LOCUS       DBNGZW010000026         3041 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_343673, whole genome shotgun sequence.
ACCESSION   DBNGZW010000026 DBNGZW010000000
VERSION     DBNGZW010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_343673"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            16..1020
                     /locus_tag="AC16JC_00640"
     CDS             16..1020
                     /locus_tag="AC16JC_00640"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR5163230.1"
                     /translation="MRRVAVIGAGAWGTTLADLLARKGLAVTIWAFESEVVQGINRDH
                     ANAMFLPDAPLAPALRATGDITEAVRQAEVVVCAVPSHAARSVMTAAAAAVSGVAPLV
                     VSVSKGLEPEAQERLSCVLARVFGHATPVAVLSGPSFAREVYERQPTAVVAAAVDHDV
                     ARRVQALFSTGSFRVYSHTDVVGVELGGALKNVIALATGIAEGLGLGYNPRAALITRG
                     LAELTRLGVAMGADPLTFAGLAGLGDLVLTATGALSRNRTLGVELGQGRRLEELLASR
                     KTVAEGVTTARTAVSLGERHGVELPIAREVSAILFAGKAPRQAIADLMERAPKPEQWR
                     "
     gene            1017..1394
                     /locus_tag="AC16JC_00645"
     CDS             1017..1394
                     /locus_tag="AC16JC_00645"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5163231.1"
                     /translation="MSAPHPVQEFFSIGEVCQLTDLKPHVLRYWESQFRFLNPAKNRS
                     GNRVYQRREIELIMLVKHLLYTEKYTIEGARAKIEQYRRSGELKPEARRALASETVKD
                     LRAELKAVLALLDGATPPHGGRS"
     gene            1378..2079
                     /locus_tag="AC16JC_00650"
     CDS             1378..2079
                     /locus_tag="AC16JC_00650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5163232.1"
                     /translation="MADAPEPERVAVIVPAYQAAENVAAVVAGARAALPGAAVYVVDD
                     GSSDATGAVATAAGAVVLRHPRNRGKGAALAGGIARALSDGATVLTTLDADGQHPPDA
                     LPRLLAPVQRGEADLVLGARARGGSMPLARRCTNWLSATVATRIGKVAVPDAQTGLRA
                     FTGRLAQELQPAIVAYRRYDYEAAFLLAALRGGYGVRSVEVPTIYNRAESHFRGWGDG
                     WRVTRVFVRYLLGAS"
     gene            2076..2825
                     /gene="surE"
                     /locus_tag="AC16JC_00655"
     CDS             2076..2825
                     /gene="surE"
                     /locus_tag="AC16JC_00655"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HIR5163233.1"
                     /translation="MNILVSNDDGVLARGLTVLADAASSLGRVTVVAPDREQSATSHS
                     LTLHRPIRATRRSDGAYQVDGTPTDCVLLGLDALMPEKPDFVLSGVNHGPNMGEDVLY
                     SGTVAAAMEGLAAGIPSVAISYGSFDLEHLESHTPWLKRLLAKIVAVPEFPRDMLLNV
                     NLPAIPGDQVKGVKVTSLGSRVFHEEVARMKDPWGRDIYWIGGGHVTWSGGADSDFRA
                     VRDGCISVTPLHVDLTNYNLLEAVRGWSLGV"
     gene            2807..>3041
                     /locus_tag="AC16JC_00660"
     CDS             2807..>3041
                     /locus_tag="AC16JC_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR5163234.1"
                     /translation="MEPGRIGGAASPDAFGGYRGRLVEQLRDQGIRDLAVLRAFAETP
                     RHLFVPDAVRHRAYENAALPIGAGQTISQPFTQA"
BASE COUNT          417 a         1021 c         1155 g          448 t
ORIGIN      
        1 gcgccggcgg gcgaggtgcg gcgggtggcc gtcatcgggg ccggagcgtg gggcacgacg
       61 ctcgccgatt tgctcgcgcg caaggggttg gcggtcacga tctgggcgtt cgagtcggag
      121 gtggtgcagg ggatcaaccg cgatcacgcg aacgcgatgt tccttccgga cgcgccgctg
      181 gccccagccc tgcgggcgac cggcgacatc accgaagccg tgcggcaggc ggaggtggtg
      241 gtctgcgccg tgccgtcgca cgcggcgcgc agcgtgatga ccgcggccgc ggccgccgtg
      301 agcggggtgg cgccgctcgt cgtcagcgtg tccaaggggc tcgagccgga ggcgcaggag
      361 cggctgtcgt gcgtgctggc ccgggtgttc ggccacgcga cgcccgtggc cgtgctgtcc
      421 ggaccctcgt ttgcccggga agtgtacgag cgccagccga ccgccgtggt cgcggcggcg
      481 gttgaccacg acgtcgcccg gcgggtgcag gcgctctttt ccacgggtag cttccgggtg
      541 tactcgcaca ccgacgtcgt cggcgtggaa ctgggcggcg ccctgaagaa cgtgatcgcc
      601 ctggcgacgg ggatcgccga agggctcggc ctgggctaca acccgcgtgc cgccctcatc
      661 acccgagggc tggccgagct gacgcgtctg ggggtcgcca tgggagcgga tccgctcacc
      721 ttcgcgggtc tggcggggct cggtgatctg gtgctcaccg ccaccggggc gctctcgcgc
      781 aaccgcacgt tgggcgtgga gctggggcag ggacggcggc tcgaggagct gctggcgagc
      841 cgcaagacgg tggccgaggg ggtgacgacg gcgcgcacgg cggtgtcgct gggcgagcgg
      901 cacggcgtcg agctgccgat cgcgcgtgag gtcagcgcga ttctgttcgc gggcaaggcg
      961 ccgcgacagg cgatcgcgga tctgatggag cgtgccccca agccggagca gtggcggtga
     1021 gcgcgccgca tccggtgcag gagttctttt cgatcggcga ggtctgccag ctcaccgacc
     1081 tgaagccgca cgtgctgcgc tactgggaga gccagtttcg cttcctgaat cccgccaaga
     1141 accgctcggg gaatcgcgtg taccagcggc gcgagatcga gctcatcatg ctggtgaagc
     1201 atctgctcta caccgagaag tacacgatcg agggagcccg ggccaagatc gagcagtatc
     1261 gacgctccgg agagctcaag ccggaggcca ggcgggcgct ggcgtcggag accgtcaagg
     1321 acctgcgcgc cgagctgaag gccgtgctcg cgctgctcga cggcgcgacg ccgccccatg
     1381 gcggacgctc ctgagccgga gcgcgtcgcg gtcatcgtgc cggcctacca ggcggccgag
     1441 aacgtggcgg ccgtcgtggc cggtgcccgc gcggcgctgc cgggcgccgc ggtctacgtc
     1501 gtggacgacg gctcgagcga tgccaccggc gcggtcgcca cggccgccgg cgcggtggtg
     1561 ctgcggcatc cgcgcaaccg cggcaagggc gcggcactcg ccgggggcat cgcccgggcg
     1621 ctgtcggacg gcgccaccgt gctgacgacg ctggacgccg acgggcagca cccgcccgac
     1681 gcgttgccgc gcctgctggc gcccgttcag cgcggcgaag cggacctggt gctgggagcc
     1741 cgggcgcgcg gcgggagcat gccgctggca cggcgctgca ccaactggct gtcggcgacc
     1801 gtcgccacgc ggatcggcaa ggtcgcggtg ccggacgccc agacgggcct gcgcgcgttc
     1861 accgggcgcc tggcgcagga gctgcagccc gcgatcgtcg cctaccggcg ctacgattac
     1921 gaagcggcat tcctcctggc ggcgctgcgc ggcggctacg gcgtccgctc ggtcgaggtg
     1981 ccgacgatct acaaccgcgc ggagagccat tttcgtggct ggggcgacgg ctggcgcgta
     2041 acccgcgtgt tcgtgcggta tctcctcggg gcatcatgaa cattctcgtc tccaacgacg
     2101 acggtgtgct cgcgcggggg ctgacggtgc tcgccgatgc ggcgagctcg ctcggccggg
     2161 tcacggtcgt ggcgccggat cgtgagcaga gcgccacctc gcattcgctg acactgcacc
     2221 ggccgattcg ggccacacgg cgcagcgacg gcgcctacca ggtggacggc actccgaccg
     2281 actgcgtgct gctggggctc gacgcgctga tgccggagaa gccggacttc gtgctctccg
     2341 gcgtgaatca cggccccaac atgggcgagg acgtcctcta ttccggcacg gtcgcggccg
     2401 ccatggaagg gctggctgcc gggattccca gcgtggcgat ctcgtacggc agcttcgatc
     2461 tcgagcacct ggaaagtcac acgccctggc tcaagcgcct gctcgcgaag atcgtcgccg
     2521 tccccgagtt tccccgcgac atgctgctga acgtcaatct ccctgcgatt ccgggtgatc
     2581 aggtgaaggg cgtgaaggtc acgagcctcg gcagccgggt gttccacgag gaagtggcgc
     2641 gcatgaagga cccctggggc cgggacatct actggatcgg cggcgggcac gtgacctggt
     2701 cgggcggggc cgactccgat ttccgggcgg ttcgggacgg gtgcatctct gtgacgccgc
     2761 tccacgtgga tctcaccaac tacaatctcc tcgaggccgt gcgcggatgg agcctgggcg
     2821 tataggaggc gccgcgagcc ccgatgcgtt cgggggctac cgcgggcgcc tcgtggaaca
     2881 gctgcgcgac cagggaatcc gggacttggc ggtgctgcgc gccttcgcgg agacgccgcg
     2941 ccatctcttc gttccggacg ccgtgcgaca ccgcgcctac gagaacgcgg cacttcccat
     3001 tggggcgggg cagacgatct cccagccgtt cacccaggcc c
//
LOCUS       DBNGZW010000027         2931 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1502582, whole genome shotgun sequence.
ACCESSION   DBNGZW010000027 DBNGZW010000000
VERSION     DBNGZW010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2931)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1502582"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="AC16JC_00665"
     CDS             complement(<1..701)
                     /locus_tag="AC16JC_00665"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR5163235.1"
                     /translation="MTPVSQSPLDALLAKAKDRSALFGIVGLGYVGLPLAMELVRAGY
                     RVLGFDVSQRVVDNLNAGRSHVQDVPASEVAAAVKDKKFSATSDLSRLAEPDAISIAV
                     PTPLSKTKDPDVSYVIAATDSIKKTLRRGQLVILESTTYPGTTRELMLPALESTGLKV
                     GEDFFLAFSPERVDPGNPKYGTRNTPKVVGGITPNCLEVTMALYQPSIERLVPVSSTE
                     AAELVKILENTFRSV"
     gene            complement(698..1702)
                     /locus_tag="AC16JC_00670"
     CDS             complement(698..1702)
                     /locus_tag="AC16JC_00670"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5163236.1"
                     /translation="MSRLKLGVIGAGAWGRNHVRTVAGLSDAELAAVCDTQEKVRQTV
                     GRQYPSAFVTEDVDALLERVEAVVIAAPAKLHAELAQRAIGAGKPCLVEKPFALGVAD
                     AEAVAAAAAARKVPVLVGHLLVFHPAVDALRALISAGELGRVLYMYGLRVNLGQVRAD
                     ENALWSFGPHDVSVALHLLGEAPVRVTAQGSSYLQPGVEDVVFLHMEFASGTVAHVQL
                     SWLDPHKERKLTVVGDRKMVVFDDMEPREKLRIYDKGVTRPPDYASWGESLAIREGDI
                     FIPKIPNVEPLGAELAHFVRVARGAEAPRASAADGVRVIRVLEAATRSLAGGGTPITI
                     "
     gene            complement(1699..2814)
                     /locus_tag="AC16JC_00675"
     CDS             complement(1699..2814)
                     /locus_tag="AC16JC_00675"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015375876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR5163237.1"
                     /translation="MNVPLLDLVGQYRAIKDEVLPALQAVIEAQQFIMGPSVAQLEAK
                     VAELSHARHGVGCASGTDALLLPLKALNLKPGEEIITSPFTFFATAGAIHNAGGTPVF
                     VDIDPGTFNIDPAAVEAAVTPRTRAIMPVHLYGQMAEMERLLAIAQRRGLKVIEDAAQ
                     AIGARRSIDGVWRMAGELGWVTGYSFFPSKNLGAWGDAGMMVTSDDVAAERLRKLRLH
                     GGAKMYHHEEVGTNSRLDTLQAAVLLAKLPHLADWSSRRREHAAYYGAGLAGVGAVRA
                     PVVAPGNEHIFHQYTLRVARRDALQAHLKAKGIGSAVYYPIPLHRQACFASLGYQAGS
                     LPRSEQAAEEVLSLPIFPELTHAQLDYVIDAIREFYR"
BASE COUNT          475 a         1041 c          962 g          453 t
ORIGIN      
        1 ttcacgctgc ggaaggtgtt ctcgaggatc ttcacgagct cggccgcctc ggtcgaggag
       61 accggaacca accgctcgat cgacggctgg tacaacgcca tcgtcacctc gaggcagttc
      121 ggcgtgatgc cgcccaccac cttcggcgtg ttccgcgtgc cgtacttggg gttgccggga
      181 tccacccgct cgggcgagaa ggccaggaag aagtcctcgc ccaccttgag cccggtgctt
      241 tcgagcgccg ggagcatcag ctcgcgcgtc gtccccggat acgtggtgga ctcgaggatc
      301 acgagctggc cgcgccgcag cgtcttcttg atggaatcgg tcgccgcaat gacgtagctg
      361 acgtcgggat ccttggtctt ggagagcggc gtcggcaccg cgatggagat cgcgtccggc
      421 tcggccagcc gcgacaaatc gctcgtcgcc gagaacttct tgtccttcac ggcggcggcg
      481 acctcgctgg ccggcacgtc ctgcacgtgg gagcggcccg cgttcaggtt gtccaccacg
      541 cgctggctga cgtcgaaccc gagcacgcgg tagccggcgc ggaccagctc catcgccagc
      601 ggcaacccga cgtagcccag gcccacgata ccgaacagag cggagcggtc cttggccttg
      661 gccaggagcg cgtccagggg agattgtgag accggtgtca tatggtgatc ggggttcctc
      721 cgcccgcgag cgagcgcgtg gcagcctcca gcacacggat cacgcgcacg ccgtccgcgg
      781 cgctcgcgcg gggagcctcg gccccacgcg cgacgcgcac gaagtgcgcg agctcggcgc
      841 cgagcggctc cacgttggga atcttgggaa tgaagatgtc accctcgcga atggcgagac
      901 tctcgcccca ggacgcgtaa tcgggagggc gcgtcacgcc tttatcgtag atacgcagct
      961 tttcacgcgg ctccatgtcg tcgaagacca ccatcttgcg gtcacccacc acggtgagct
     1021 tgcgctcctt gtgcgggtcc agccatgaca gttggacgtg cgccacggtc ccgctggcga
     1081 actccatatg gagaaacacc acatcctcaa cgcccggctg cagatagctc gacccctgcg
     1141 cggtgacgcg aaccggcgcc tcccctagca aatggagggc cacggagaca tcgtgcggtc
     1201 cgaagctcca cagggcgttc tcatccgccc gcacctgccc caggttcacc cgcagcccgt
     1261 acatatagag cacccgcccc agctcccccg cactgatcag cgcccgcagc gcatccaccg
     1321 cggggtggaa cacgagcagg tgccccacca ggaccgggac cttccgggca gccgccgcgg
     1381 cggccaccgc ctcggcatcg gcgactccca gggcgaacgg cttctccacc aggcacggct
     1441 tccccgcccc gatcgcccgc tgggccagct ccgcgtgcag cttggcgggc gcggcgatga
     1501 cgaccgcctc gacccgctcc agcagggcgt ccacatcctc ggtcacgaag gcggacgggt
     1561 actggcgtcc gaccgtttga cgcactttct cctgcgtatc acacacggcc gcgagctcgg
     1621 catcggagag cccggccacc gtgcgcacat ggtttctgcc ccatgccccg gcgccgatga
     1681 cgccgagttt cagccggctc accggtagaa ctcgcggatc gcgtcgatca cataatcgag
     1741 ctgtgcgtgg gtgagctccg ggaagatggg aagcgacagg acctcctcgg ccgcctgctc
     1801 ggagcgcggc aggctgcccg cctggtaccc gagggacgcg aagcacgcct gccggtgcag
     1861 cgggatcggg taatagaccg ccgagccgat gcccttggcc ttgagatgag cctggagggc
     1921 gtcccgacgc gctacccgca gcgtgtattg atggaagatg tgctcgttcc ctggggccac
     1981 gaccggcgcc cggaccgcgc ccaccccggc gagtccggcc ccgtagtatg ccgcgtgctc
     2041 ccggcgccgg ctcgaccagt ccgccaggtg cggcagcttg gcgagcagca ccgcggcttg
     2101 cagcgtgtcg agccgcgagt tcgtccctac ttcctcgtga tggtacatct tggctccgcc
     2161 gtgcagccgc agcttgcgca accgctcggc cgccacgtcg tcggaggtca ccatcatgcc
     2221 ggcgtcgccc caggcgccca ggttcttgct cgggaagaac gagtagcccg tcacccaccc
     2281 cagctcgccg gccatgcgcc acacgccgtc gatcgagcgg cgcgcgccga tcgcctgagc
     2341 agcgtcctcg atcaccttca ggccgcgccg ctgggcgatc gccagcagcc gttccatctc
     2401 cgccatctgc ccgtagaggt ggaccggcat gatggcacgc gtccggggcg tgaccgccgc
     2461 ctcgaccgcc gccggatcga tgttgaacgt ccccgggtcg atgtccacga agaccggcgt
     2521 gccgccggcg ttgtggatcg cgcccgccgt cgcgaagaag gtgaacggcg aggtgatgat
     2581 ctcctctccg ggcttgaggt tgagcgcttt gagcggcagc agcagggcgt cggtgccgct
     2641 ggcacagccg acaccatggc gggcgtgcga cagctcggcc accttggcct cgagctgcgc
     2701 cacggacggt cccatgatga actgctgcgc ttcgatgacc gcctgaaggg cgggaaggac
     2761 ctcgtccttg atggcccgat actggccgac caggtccagc aacggcacgt tcacgtagac
     2821 tctatccgca aaagaggcaa caatttacac agagggggcg ggttcaggca atcgggcgca
     2881 gcacgccccc ggtttcctca taggaggtgc cgcaggaggc gcagcgggcg c
//
LOCUS       DBNGZW010000028         3199 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_480500, whole genome shotgun sequence.
ACCESSION   DBNGZW010000028 DBNGZW010000000
VERSION     DBNGZW010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3199)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3199)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3199
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_480500"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..375)
                     /locus_tag="AC16JC_00680"
     CDS             complement(<1..375)
                     /locus_tag="AC16JC_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163238.1"
                     /translation="MFLQVGRALPSSPWDLVLASSRETKFVLAVLVVFSVISGYLIIL
                     KWWQFRHMRRQADRFMREIEKANRLEEAYHTAMRLPPSPYNRLLREAVQFFSHLKPGL
                     LREPAGPTPTPTLTMMQLEALRM"
     gene            complement(377..1321)
                     /locus_tag="AC16JC_00685"
     CDS             complement(377..1321)
                     /locus_tag="AC16JC_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5163239.1"
                     /translation="MTRRVLVSGGAGFIGSHVVEAYLAAGDEVSVLDDLSTGKVENVP
                     AGAKLVRADIRSPEARALVAGGGFTILNHHAAQMDVRRSVEDPVFDAQVNVLGLLNLL
                     EGARAGGVGRVVFASSGGTVYGEGAPTPTTEPAAKLPASPYGTAKLTSEYYLATFARL
                     YGIEAVALRYANVYGPRQNPHGEAGVVAIFGKRLLKGEPITIYGSGEQTRDLVYVEDV
                     AAANLLAAERPLPALQSLDSRAFNIGTGIETSVNRLADLIVQATGRPGDVRRAPARPG
                     EILRSALAVAKAERELDWRPRVPLPDGLARTMRWIAEG"
     gene            complement(1431..2585)
                     /locus_tag="AC16JC_00690"
     CDS             complement(1431..2585)
                     /locus_tag="AC16JC_00690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR5163240.1"
                     /translation="MRRTTICRSSRIMAAVVVALPVAATLAAQDTTTVAPAAGSHVVA
                     RGETLWSISQQYFADPLLWPEIYRRNTAVIEDPHWIYPGEVLDLGGIAGAPVDTTQVV
                     GQGIADTTGQADTIGAIPEPPPEEPEGPGTIFDRRSSKQQQVQDVLRRYAHQIYRPVR
                     RGEFYSSGFLTEGEDLPWARVVGNTAKPSIPVLTDPGTAGQFAEIAIVPPSQASYHVG
                     DSLLVARLDRKIEPWGDVVVPQGVARVTSVQEKQVLGLVIMQFGRIQGGSLALPLEPF
                     KDPGSVRPASVEQGLEGHLIAMRDEHVLASVQQVLFIDKGRSDGVAMGDVFEAYRPAS
                     GEVGARSEEVRVQLLVVHTREHTASVIVIGMSNPALGSGMPVRLIKKMPS"
     gene            complement(2557..3036)
                     /locus_tag="AC16JC_00695"
     CDS             complement(2557..3036)
                     /locus_tag="AC16JC_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR5163241.1"
                     /translation="MSRSLNKVMLIGNLGADPEVRSTNNGSRVANLSLATSRQWTGQN
                     GEKQEKTEWHRVICWNNKGFQLADLAEKYMKKGDKIYVEGRVEYRTWEDKEKQTRYVT
                     EIIAREVILLSPRGAGSEGSGDFARAKATAKVAPEGKTESLDAFPEALDEEDDDLPF"
BASE COUNT          506 a         1129 c         1046 g          518 t
ORIGIN      
        1 catacgcaag gcctcgagct gcatcatcgt cagcgtcggc gtgggggtcg gaccggcggg
       61 ctcgcgcagc aggccgggct tcaggtgcga gaagaactgc acggcctcgc gcagcaggcg
      121 attgtacggc gacggcggca agcgcatcgc cgtgtgatac gcctcttcga ggcggttggc
      181 cttctcgatc tcccgcatga agcgatcagc ctgccggcgc atgtggcgga actgccacca
      241 tttcaggatg atcaggtacc cggagatgac cgagaacacc accagtaccg cgaggacgaa
      301 cttcgtctcg cggctcgagg ccagcaccag atcccagggc gaggacggca gcgcccggcc
      361 gacttgcagg aacaccctac ccctccgcga tccagcgcat ggttcgtgcg agcccgtccg
      421 gcagcggcac ccgggggcgc cagtccagct cgcgttccgc cttcgcgacc gccagggcgc
      481 tccgcagaat ctcgccgggg cgcgccggcg cccggcggac atcgcccgga cggccggtgg
      541 cctgcacgat caggtcggcg agacggttca ccgaggtctc gatgcccgtc ccgatgttga
      601 aggcccggct atcgagcgac tgcagcgccg gcagcgggcg ctcggccgca agcaggttgg
      661 ccgcagccac gtcctcgacg tacaccagat cgcgcgtctg ttccccgctc ccgtagatcg
      721 tgatcggctc gcccttgagg agccgcttgc cgaagatggc caccacgccg gcctccccgt
      781 gcggattctg ccgtggcccg tacacgttgg cgtagcggag cgccacggcc tcgatcccgt
      841 acagacgggc gaacgtcgcg agatagtact ccgaggtcag cttggcggtc ccataggggg
      901 aggccggcag tttggcggcg ggttcggtcg tcggagtcgg ggcgccctca ccgtacaccg
      961 tgccgccgga agaggcaaag acgacgcggc ccaccccgcc cgcccgtgcc ccctcgagca
     1021 ggttcaggag gccgagcacg ttgacctggg catcgaagac cggatcctcc acggaccgcc
     1081 gcacgtccat ctgcgcggcg tggtgattga ggatggtgaa cccgccgccg gcgacgaggg
     1141 cgcgggcttc cggcgaccgg atatcggcac gcaccagctt cgcgccggcc ggcacgtttt
     1201 ccacctttcc cgtggacagg tcgtcgagga cgctcacctc gtctccggcc gcgaggtatg
     1261 cctccacgac gtgcgacccg atgaacccgg cgccacccga gaccagcacc cgtcgtgtca
     1321 tgtgcgtgcg cttcctctcc tacctccggt tcggccggcc gccgcggagg cgacgatggt
     1381 gggatgatga tcggcggccc cggtcgagtg accggggccg gcaggcgagg tcaggaaggc
     1441 atcttcttga tgaggcgcac gggcataccg gagccaaggg cgggattcga catgccgatg
     1501 acgatcaccg aggcggtgtg ctcgcgcgtg tgcaccacca ggagctgcac ccggacttcc
     1561 tcggagcgcg ccccgacttc gccggacgcg ggccgatacg cctcgaacac gtcgcccatt
     1621 gcgacgccgt cggagcgtcc cttgtcgatg aagagcacct gctgaacgct cgccagcacg
     1681 tgctcgtcgc gcatcgcgat gagatgccct tcgagaccct gctcgaccga agccggccga
     1741 acggagccgg gatccttgaa gggttccagc ggcagcgcca ggctgccgcc ctggatgcgc
     1801 ccgaactgca tgatgaccag ccccagcacc tgcttctcct ggaccgacgt cacgcgggcg
     1861 acgccctgcg gcaccaccac atcgccccac ggctcgatct tgcgatccag ccgggcgacg
     1921 agcagcgaat cacccacgtg atacgacgcc tgcgacggcg gcacgatcgc gatctccgcg
     1981 aactgaccgg ccgtccccgg gtcggtgagc accgggatcg agggtttggc ggtattgccg
     2041 acgacgcgcg cccacgggag atcctctccc tcggtcagga accccgagga gtagaactcg
     2101 ccgcgccgca ccggccggta gatctggtgc gcgtagcgcc gcagcacatc ctgcacctgc
     2161 tgctgcttgc tcgagcgacg atcgaagatc gtgcccggcc cctccggctc ctcgggcggc
     2221 ggctcgggga tcgccccgat cgtgtcggcc tggcccgtgg tatcggcaat gccctggccc
     2281 accacctggg tggtatcgac cggcgcgccg gcgatgccgc ccagatcgag cacctcaccg
     2341 ggatagatcc agtgcgggtc ctcgatcacc gcggtgttgc ggcggtagat ctccggccac
     2401 aacagcgggt cggcgaagta ctgctgcgag atcgaccaga gagtttcacc ccgcgccacg
     2461 acatgacttc cggcggcggg ggcgaccgtg gtggtgtcct gggcagcgag cgtcgccgcc
     2521 accggcaggg cgaccaccac ggccgccatg atgcggctag aacggcagat cgtcgtcctc
     2581 ctcatccagc gcctccggga acgcgtcgag cgactcggtc ttgccttccg gcgccacctt
     2641 cgccgtcgcc ttggcgcgcg caaaatcccc tgacccctcg cttccggcgc cacggggcga
     2701 caacaggatc acttcacgcg cgatgatctc ggtgacgtaa cgggtctgct tctctttgtc
     2761 ttcccaggtg cggtactcga ctcgcccttc gacgtagatc ttgtcgccct tcttcatgta
     2821 cttctcggcg aggtcggcga gctggaagcc cttgttgttc caacagatca cccggtgcca
     2881 ctcggtcttc tcttgcttct cgccgttttg gccggtccac tgacggctcg tggccaggga
     2941 cagattggcc acacgggacc cgttgttggt gctacgcact tcgggatcgg cccccaggtt
     3001 gccgatcagc atgactttgt tcaggcttct gctcacggct gcctccggac gttgggcggg
     3061 cgggggccaa ggatgcccca gattatatga cccgcaagtc attctgtcaa tcaaagatac
     3121 gccacttgta ccatttctac gcagacggct cgactgccgg aatggcggcc cggaggatca
     3181 ccgcgtcctc caccggccg
//
LOCUS       DBNGZW010000029         1825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_889211, whole genome shotgun sequence.
ACCESSION   DBNGZW010000029 DBNGZW010000000
VERSION     DBNGZW010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1825
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_889211"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="AC16JC_00700"
     CDS             complement(<1..546)
                     /locus_tag="AC16JC_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5163242.1"
                     /translation="MDKHTDRRGFLRIAAGAGPAALAAACAWDGGDLIRPRLLSLSRL
                     NDWVGEKILFSPTRLAPTYDPALRAPSLPNYHISDSTPLLRDPASWRLTVDGLVEQPL
                     SLSLDDLHRMPRASYTVKHHCVEGWSAIASWAGVAVSEVVLRARPKPAARYILFRSFD
                     SDYSNGWDMASAMHPQTILAYA"
     gene            complement(539..1294)
                     /locus_tag="AC16JC_00705"
     CDS             complement(539..1294)
                     /locus_tag="AC16JC_00705"
                     /inference="COORDINATES: protein motif:HMM:NF013460.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR5163243.1"
                     /translation="MSAPTTPTPAPGPEPAPVPAPEATPAAPPPRTRGVRRHHWIVRL
                     THWATFVVLAGMIASGLQIYEAYARFGNRGTPSYPNPFEGARFDRAIRLGGWLAGGLN
                     WHFALMWPLVLFGLLYASYLVGSGEWRSLLFRPKDVPGAIEMTKYYLRLRKEHPPQGK
                     HNPLQKLAYTTVVLLGVLALLTGLAIYKPTQLSWLTALFGGFQAARYWHFWIVWIFVA
                     FSVTHVLLVFLVDPASLRAMLTGRYRGRFPSHG"
     gene            1404..>1825
                     /locus_tag="AC16JC_00710"
     CDS             1404..>1825
                     /locus_tag="AC16JC_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163244.1"
                     /translation="MRRVWLPLLALAACRPSPRPARRAAGGTAHPDSAGLSSLWRAPR
                     DSIHDRLLRVPRIVTAPSVVVFWLRSGDTLNRDSAQAASRDLDFYTEQVAQTLARNGI
                     MLVPTNADTVYVELADHQRRAIVLSGLDYPYGYLLIDPG"
BASE COUNT          280 a          615 c          667 g          263 t
ORIGIN      
        1 ggcgtacgcg agaatcgttt gcgggtgcat cgcgctcgcc atgtcccagc cgttggagta
       61 gtcggaatcg aagctgcgga acaggatgta gcgcgccgcc ggcttggggc gggcccgcag
      121 cacgacctcg ctcaccgcga caccggccca cgacgcgatc gcgctccacc cctccacgca
      181 atggtgcttc accgtgtaac tcgcgcgggg catgcggtgg agatcgtcga gcgacagcga
      241 gagcggctgc tcgaccaggc cgtccaccgt gagacgccag ctcgcgggat cgcgcagcag
      301 cggcgtcgag tcggagatgt ggtagttggg gaggctcggc gcgcggagcg cgggatcgta
      361 ggtcggcgcc agccgcgtcg gcgaaaagag aatcttctcg cccacccagt cgttgagccg
      421 gctgagcgag agcagtcgtg ggcggatcag gtcgccacca tcccaggcgc aggcggcggc
      481 cagcgcagcg gggccggcgc cggcggcgat gcgcaggaac ccacggcggt cggtgtgctt
      541 atccatgact cggaaacctc ccccggtagc gaccggtcag catcgcgcgc agactggcag
      601 ggtccaccag gaagaccagc agcacgtggg tgacgctgaa cgcgacgaag atccacacga
      661 tccagaagtg ccagtagcgc gccgcctgga agccgccgaa cagcgcggtg agccaggaga
      721 gctgcgtcgg cttgtagatc gccaggccgg tgagcagggc gagcacgccc agcagcacga
      781 cggtcgtgta ggcgagcttc tgcagcgggt tgtgcttccc ctggggcggg tgctccttgc
      841 gcagccgcag gtagtacttc gtcatctcga tcgccccggg gacgtccttc ggccggaaga
      901 gcagggaccg ccactcgccg ctgcccacga ggtaggaggc atagagcagg ccgaagagga
      961 cgaggggcca catcagggcg aagtgccagt tcaggcctcc ggcgagccag ccacccagcc
     1021 ggatcgcgcg gtcgaagcgg gcgccctcga acgggttggg gtagcttggg gtgccccggt
     1081 tcccgaaccg ggcgtaggcc tcgtagatct ggagcccgct cgcgatcatc ccggcgagaa
     1141 cgacaaatgt ggcccagtgg gtgaggcgga cgatccagtg gtgtcgccgg acgccccgtg
     1201 tccgcggtgg tggcgccgct ggagtggcct ccggagccgg tactggggct ggctcgggac
     1261 ccggggcggg ggtgggggtg gtcggtgcgc tcatggccgt caatgtacgc cttaatgcac
     1321 ccggcgcccg agtggttccc tgggccggcg cggtgggtcg gtggtcacac ggcaagaccc
     1381 ggacattcat tatcttcccc cccgtgagac gcgtctggct ccccctcctg gccctggcgg
     1441 cctgccgccc ctcgccccgc cccgcccggc gggccgccgg gggcacggcg caccccgaca
     1501 gcgcgggact gtcttccctg tggcgcgctc cgcgcgattc gatccacgac cgcctgctgc
     1561 gggtgccgcg catcgtgacc gcgcccagcg tggtggtgtt ctggctgcgc tcgggggaca
     1621 cgctgaaccg ggactccgcg caggccgcct cgcgcgacct cgacttctac accgagcagg
     1681 tggcccagac cctggcgcgc aacggcatca tgctcgtgcc gaccaacgcc gacacggtgt
     1741 acgtggagct ggccgatcac cagcggcgcg cgatcgtgct gtcggggctc gactatccct
     1801 acgggtatct gctgatcgat cccgg
//
LOCUS       DBNGZW010000030         2446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_3206, whole genome shotgun sequence.
ACCESSION   DBNGZW010000030 DBNGZW010000000
VERSION     DBNGZW010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_3206"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="AC16JC_00715"
     CDS             <1..843
                     /locus_tag="AC16JC_00715"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR5163245.1"
                     /translation="RGRHRDILSPLIRNLGALLVMGARPIEILRQYMAKAESPTGGRE
                     LNVHFNDLEKGYLGQISHLGDMVPVMAGITLTFKMRAEPRVGLVFVGDGATSTGAFHE
                     GLNFAAVQRCPLVVIGEYNHWAYSTPPEKQFAVQHLADKAKGYGIHAVTVDGNDVLAV
                     YAATKYAVERARRGKGVHFIEVKTYRRKGHAEHDDQSYQPKDEIERWARENDPVDRFV
                     KQVLDNDWAEEAELAEIDRAVKDEVDQATDACVNEPLPAPESALPGVYAHPAAAERLW
                     FRSL"
     gene            843..1820
                     /locus_tag="AC16JC_00720"
     CDS             843..1820
                     /locus_tag="AC16JC_00720"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR5163246.1"
                     /translation="MAEVTFLEALRQGLWEEMERDERVFMLGEDIGAYGGAFKVTEGF
                     QKRFGEARVIDTPISEAAIVGAAAGAAHMGMRPVAEMQFIDFISCAYDMLTNYVATAR
                     YRAGLATPIVVRGPCGGYVRGGPFHSQNPEAAFLHTPGLKIVYPATARDAKGLIKASI
                     RDEDPVLYLEHKWLYRRIKETLPDGEEVLTPIGQARLAREGKDVSVITYGATVWKALE
                     AADRLAQDGVSVEVLDLRTLLPLDDDAIVATVQKTGKVLVVHEDTRTGGLAGEITARI
                     NEQAFEWLDGPIRRVTAHDVPLPYAPTLEDFVLPQTDDIVNAARWLAKY"
     gene            1916..>2446
                     /locus_tag="AC16JC_00725"
     CDS             1916..>2446
                     /locus_tag="AC16JC_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018545504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR5163247.1"
                     /translation="MARIDVLMPQMGESIAEGTLSKWLKKLGDTVKRDEPLFEISTDK
                     VDAEIPAPSAGTLVEIKVQEGQTVPVQTLVAVLDTEQGAAVSSSAPAAAPAASAAPKA
                     APAPAAKPAAPAPAPVAAATAAAAAAPPVPRPTSPVPPASPVPPKGDGQETAEERLRR
                     RSTPLVRKIATEHQLDI"
BASE COUNT          418 a          816 c          866 g          346 t
ORIGIN      
        1 cgcggccgtc atcgcgatat cctctcgccc ctgatccgca acctcggcgc gctgctcgtg
       61 atgggcgcca ggccgatcga gatcctgcgc cagtacatgg ccaaggcgga gagccccacg
      121 ggcggacgtg agctgaacgt ccacttcaac gatctcgaga agggctatct ggggcagatc
      181 tcccacttgg gcgatatggt cccggtgatg gcggggatca cgctcacctt caagatgcgc
      241 gccgagccgc gcgtcggcct cgtgttcgtg ggcgacggcg cgacctcgac gggcgcgttt
      301 cacgaagggc tgaacttcgc cgcggtgcag cgctgccccc tcgtcgtcat cggggagtac
      361 aaccactggg catactccac gccacccgag aagcagttcg cggtgcagca cctcgcggac
      421 aaggcgaagg gctacgggat ccacgccgtc acggtggacg gcaacgacgt gctcgctgtg
      481 tacgcggcga cgaaatacgc ggtggagcgt gcgcgtcgcg gcaagggcgt gcacttcatc
      541 gaggtgaaga cctaccgccg caaggggcat gcggagcacg atgatcagtc ctatcagccg
      601 aaggacgaga tcgagcgctg ggcgcgggag aacgatcccg tcgaccggtt cgtgaagcag
      661 gtgctcgaca acgactgggc cgaggaggcc gagctggccg agatcgaccg tgcggtcaag
      721 gacgaggtcg accaggcgac ggacgcctgc gtcaacgagc cgttgccggc gcccgaatcg
      781 gcgctgcccg gcgtctacgc gcaccccgcg gccgccgagc ggctgtggtt ccggagtctg
      841 taatggccga ggtcacgttc ctcgaggctc tgcgccaggg actgtgggaa gagatggagc
      901 gcgacgagcg cgtgttcatg ctcggcgagg acatcggggc ctacggcggc gccttcaagg
      961 tgaccgaggg gttccagaag aggttcggcg aggcgcgcgt catcgatacg ccgatctcgg
     1021 aggccgcgat cgtgggtgcg gcggccgggg cggcgcacat ggggatgcgg ccggtcgcgg
     1081 agatgcagtt catcgacttc atctcctgcg cctacgacat gctgacgaac tacgtggcga
     1141 ccgcgcgcta tcgcgccggc ctcgccaccc cgatcgtcgt gcgcggcccc tgcggcgggt
     1201 atgtgcgcgg cggcccgttc cattcgcaga atcccgaggc ggcgtttctg cacacgcccg
     1261 gcctaaagat cgtctatccg gcgaccgcgc gcgacgcgaa gggcctcatc aaggcgtcga
     1321 tccgggacga ggatcccgtg ctctatctcg agcataagtg gctgtaccgc cgcatcaagg
     1381 agacgctgcc ggacggtgag gaagtgctca cgccgatcgg ccaggcgcgg ctcgcccggg
     1441 aggggaagga cgtgtcggtg atcacctacg gggcgaccgt ctggaaggcg ctcgaggcgg
     1501 ccgaccggct ggcgcaggac ggggtgtcgg tcgaagtgct cgacctgcgc acgctgttgc
     1561 ccctcgacga cgacgcgatc gtcgccacgg tgcagaagac ggggaaggtg ctggtcgtcc
     1621 acgaggatac ccggaccggc gggctggcgg gcgagatcac cgcgcgcatc aacgagcagg
     1681 cgttcgagtg gctcgacggg ccgatccggc gggtgacggc acacgacgtg ccgctgcctt
     1741 acgcgccgac gctggaagat ttcgtgctgc cgcagacgga cgacatcgtc aacgcggcaa
     1801 gatggctggc gaaatactag agtcgtcatc ggtcgacggt cgtcggtcga caaccggcgc
     1861 ccaggaccga cgcgacgacc gatgaccgac gaccgttgac gattccgagg gagctatggc
     1921 ccgcatagac gtcctcatgc cccaaatggg agagtccatc gccgaaggga ctctcagcaa
     1981 atggctcaag aagctcggcg acacggtgaa gcgcgacgag ccgctgttcg agatctcgac
     2041 cgacaaggtg gacgccgaga ttccggctcc ctccgcgggg acgctggtcg agatcaaggt
     2101 gcaggaaggc cagacggttc cggtgcagac gctcgtggcg gtgctggaca cggagcaggg
     2161 ggcggccgtc agcagctctg cgcccgccgc cgcacccgcg gcctccgccg ctccgaaagc
     2221 ggcgccagca cccgcggcga agcccgcggc tcccgcgccg gccccggtcg ccgctgccac
     2281 tgctgctgcc gcggccgcac cacccgtccc tcgtcccacg tcccccgtcc ctccggcgtc
     2341 ccccgtccct cccaagggcg acggtcagga gaccgccgag gagcgcctgc ggcgccgctc
     2401 gacgccgctg gtccgcaaga tcgcgaccga gcaccagctc gacatc
//
LOCUS       DBNGZW010000031         7776 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_957913, whole genome shotgun sequence.
ACCESSION   DBNGZW010000031 DBNGZW010000000
VERSION     DBNGZW010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7776)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7776)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7776
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_957913"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1710
                     /gene="groL"
                     /locus_tag="AC16JC_00730"
     CDS             73..1710
                     /gene="groL"
                     /locus_tag="AC16JC_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129558.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HIR5163248.1"
                     /translation="MAAKYLEFNTEARSRLKRGVDQLAEAVKVTLGPKGRNVVIDKKF
                     GNPTVTKDGVTVAKEIELEDEIENMGAQMVKEVATKTSDLAGDGTTTATVLAQAVYRE
                     GLKSVTAGVNPMSLKRGIDKAVEAVVEELKRISVPTKGRKEIAQVGSISANGDKEIGD
                     KIADAMDKVGKDGVITVEEAKGLETTLETVDGMQFDRGYLSPYFITDPEKMEAVLEDA
                     YILVHDKKISAMKDLLPVLEKVAQSGKPLLLIAEDVEGEALATLVVNKLRGTLKVCGV
                     KAPGFGDRRKEMLTDISVLASGKVISEELGFKLENTVLGDLGQAKRIVVDKDNTTIIG
                     GKGKQVDIQGRIAQIKSAIEKSTSDYDKEKLQERLAKLAGGVAVINVGAATETEMKEK
                     KARVEDALHATRAAVEEGIVPGGGVALLWCQKTLDKVRGADEDEKIGIDIVRRALEEP
                     IRMIAQNAGAEGSIIVGKVKESAKLNYGYNAQTDEYEDLVAAGVIDPTKVTRTALQNA
                     ASIAGLLLTTECVVVEKKEKEKAPPMPGGAGGMGGMY"
     gene            1832..3961
                     /locus_tag="AC16JC_00735"
     CDS             1832..3961
                     /locus_tag="AC16JC_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR5163249.1"
                     /translation="MVTSPGRAAPGAGGPAPERAALSRELSEFLIELSIALHKHAMYP
                     LGHPSLGPAVSRVVRRGEALLQDRPSLSLGVARNQLVIEGVATDPKHPVLAELAGRLH
                     RHHLGAVTFSRGIEPGEVADLLRTLAVEADRTGQPLGMGDPAKLSAWKHARLHPMTYE
                     RLELLDQAPAVEPDTAAEARSGDEATLRDARVRGAQLWIGLARAALAGQIGEDEAPPT
                     RPAVIAKAIDEHAGGEAYDQVIVGYLLQIAQELKAAGGQEALELRRRTSRLLSEMQPE
                     TLRRLIQMGGDTAQRRQFVLDASHGMAVDAVLEVVKAAADASQHAVSSSLMRMLSKLA
                     THAEQGTTQARSLADHELRDQVRSLLTGWELEDPNPAAYGAALQRLSQARPTMVAPTE
                     RMHAAEPERLVQMSLEVNAVGPHVYRACAELVDSGRLPLLIDLLEQAPGTAAAEELWN
                     RAVSPDTVRWLLQHEPADFVTVDRVLPRLAQQAAEPLLDALADSEVRATRRAILERLV
                     RLGPVLGQSITARLSDERWYVKRNLLTLLDLLPELPPGFSALQWLGDSDPRVRVEALK
                     LGLKSPAERDRVITRGLQDTEHRIVRLALLAAFQGVPADAVPQLVALVNNGTLPPDMR
                     VPAIRLLGAARSPQALETLLALADGGRTLLGRRRLPPKSRELLTALRAPASGWRTEPR
                     AMEVLLVAAVSSDAQIRAATDPAGAAV"
     gene            3958..5196
                     /locus_tag="AC16JC_00740"
     CDS             3958..5196
                     /locus_tag="AC16JC_00740"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR5163250.1"
                     /translation="MSEPAEFLGAFAQALAGMMLYAEGHPARARGIDAAYEELLDLQL
                     ETPRPLFTFLGDEVIFGRLPLREMRSWDWSMRLAEAGIQRLEFMERVSREDFEAFLED
                     VLARVTMTPIDTAEARQLRPTSIKFGAVGVRSGAGDLPQEEALTTATIAFSLGEEAET
                     IRWLHREVQEREALPLAEAEAVVRSLSVAMHGDQQMMLPLLQLKQFDQYTTTHSLNVA
                     VLSMALAEFLGMGAADVRAFGVAGLLHDLGKVRIPIDVLTKPGKLTPDERVLMNTHPA
                     EGARIILESEQELDLAAVVAYEHHIMLNGLGYPQVKFRRPCHEASNLIHVCDVYDALR
                     TRRPYREAWSVEQVLPYIEGRAGLEFDPAYVRAFIAMMRKWDSQVAVVSDERAPLPAS
                     AAAAETGSAGPEASPSSHPV"
     gene            5213..5596
                     /locus_tag="AC16JC_00745"
     CDS             5213..5596
                     /locus_tag="AC16JC_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163251.1"
                     /translation="MDKTSLLLAMCTLGATGLSAQRATVPRLEGKWEARTDDGPRLIM
                     VRADSSAQFGDQVARWRMVGDSVWFTLGDGVWNVYGLKVAGDKLTISGGDLEKPVTLR
                     RVGPPTPRPDTLKVPEPPPASARAW"
     gene            complement(5680..6849)
                     /locus_tag="AC16JC_00750"
     CDS             complement(5680..6849)
                     /locus_tag="AC16JC_00750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163252.1"
                     /translation="MSLALYLLDPDPAPVWAPFAGVRPLSELRAGAHLIRERWETFVG
                     AEADAVFALSHLAGFAEPGTPPVMPRRAVTGPAVIGSSTFAPQGVAPELPHVPVRLVS
                     HGITVGWGVPAGVVWEGPQPQAQAIEVPGVVLHGTFDLIPALLQLLPDDLRQMLGDSD
                     PVPAGSILIGDPAALELHEAQVEPGVVFDVSHGPVVVESGVEIRSGTRLEGPLWVGAN
                     ARVLGGDLRGSAVGPRCTVHGEVASCVFLGYANKSHDGFLGHSVVGRWVNCGAGTITS
                     NLKNTYGPVRLEIPGARVDTAQQYLGTLFGDHAKTAIGTLLGTGSVVGAGANVFDAVR
                     PPKYVAPFAWGGASRERMTREGFLRIVERVLPRRDVEVTDELRAVLGRIYDWSTK"
     gene            complement(6846..>7776)
                     /locus_tag="AC16JC_00755"
     CDS             complement(6846..>7776)
                     /locus_tag="AC16JC_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR5163253.1"
                     /translation="LPLAGDRPLLSQAVRRLEGLVPPDRVLILTGPFLVERVTELVPE
                     VPRAQVLAEPRSASTAPALVWGAHWIARRDPDAQMLSLHADWAIGDEAAFRTTAARAL
                     AVAREHDVLVTVGVKPTRPESAYGYIVPGRPLGRLARRVQRFVEKPTAARAAELRRRK
                     ALWNTGLFAWGVERFLAEASAHARELRAGLPALSSGDVAGFFAAVTPAPVDIAVLERS
                     KRMAVVAGTFVWDDIGSWDALFRIRTPDSQGNVAVGNITIGDDVRRCVLWSESEHLAV
                     SGITDLVVVRANGHTLVMPTGRADRLKTLVQRL"
BASE COUNT         1295 a         2625 c         2785 g         1071 t
ORIGIN      
        1 ggggaagaga tcattctgat caaggaagcc gacgtcatcg cgaagctcag ctaaccaggc
       61 cgggaggaat agatggctgc caagtatctg gagttcaaca ccgaggcccg ctcccgtctg
      121 aagcggggcg tggatcagct ggccgaggcg gtgaaggtga cgctcggtcc gaagggccgc
      181 aacgtagtca tcgacaagaa gttcgggaat cccacggtca ccaaggacgg cgtgaccgtc
      241 gccaaggaga tcgagctgga agacgagatc gagaacatgg gcgcccagat ggtgaaggaa
      301 gtggcgacca agacctcgga tctcgccggt gacggcacga ccaccgcgac ggtgctggcg
      361 caggccgtgt accgcgaagg gctgaagtcg gttacggcgg gcgtcaatcc gatgtcgctg
      421 aagcgcggca tcgacaaggc cgtcgaggcg gtggtggagg agctgaagcg gatctccgtt
      481 cccaccaagg gccgcaagga aatcgcccag gtcgggtcga tctcggccaa cggcgacaag
      541 gagatcggag acaagatcgc cgacgccatg gacaaggtcg gcaaggacgg cgtgatcacc
      601 gtcgaagagg cgaaaggcct cgagaccacg ctcgagaccg tggacggcat gcagttcgac
      661 cgcggttatc tctcgcccta cttcatcacc gatcccgaga agatggaagc cgtgctggag
      721 gatgcctaca tcctggtgca cgacaagaag atctcggcga tgaaggacct gctcccggtg
      781 ctggagaagg tggcgcagtc gggcaagccc ctgctgctga tcgccgagga cgtcgaggga
      841 gaggcgctcg ccacgctggt ggtcaacaag ctgcgtggca cgctgaaggt ctgcggtgtg
      901 aaggccccgg gcttcggtga ccgccgcaag gagatgctca ccgacatctc ggtgctcgcg
      961 tccggcaagg tgatttccga ggagctgggc ttcaagctcg agaacacggt gctgggcgac
     1021 ctcggccagg cgaagcggat cgtggtggac aaggacaaca ccaccattat cggcggcaag
     1081 ggcaagcagg tcgacatcca gggccggatc gcgcagatca agtccgccat cgagaagtcg
     1141 acgtcggact acgacaagga gaagctgcag gagcggctcg cgaagctcgc gggcggggtc
     1201 gccgtgatca acgtcggcgc ggccaccgag accgagatga aggagaagaa ggcccgggtg
     1261 gaggatgcgt tgcacgccac ccgcgccgcg gtcgaggagg gcatcgtgcc gggcggcggc
     1321 gtcgcgctgc tgtggtgcca gaagacgctc gacaaggtgc gcggggcgga cgaggacgag
     1381 aagatcggga tcgacatcgt ccggcgcgcg ctcgaggagc cgatccgcat gatcgcccag
     1441 aacgcgggcg cggaaggctc gatcatcgtc ggcaaggtga aggagtcggc caagctgaac
     1501 tacggctaca acgcccagac cgacgagtac gaggacctgg tggcggcggg cgtcatcgac
     1561 cccaccaagg tgacgcggac ggcgctgcag aacgcggcgt ccatcgccgg gctgctgctc
     1621 accaccgagt gcgtcgtggt ggagaagaag gagaaggaga aggccccccc gatgcccggt
     1681 ggcgccggcg gcatgggcgg gatgtactga taggctaggc gtacagacgt acagacgtac
     1741 aggcgtacag acgaggcacc tccggaagac tccgaggtgc ctcgtttttc gtctggacta
     1801 tccccgaatg cattgccatt tagtatgtta gatggtgacc tcgcccggac gagctgcacc
     1861 tggcgcagga ggccccgcgc cggaacgtgc cgcgctgtcg cgcgagctca gtgagttcct
     1921 catcgagctc tcgatcgccc tccacaagca cgccatgtac ccgctcgggc atccgtctct
     1981 ggggcccgcc gtgtcgcgcg tcgtccgtcg tggcgaggcg ctgctgcagg accgcccgtc
     2041 gctgtcgctc ggcgtggcgc gcaaccagct cgtgatcgaa ggcgtcgcga ccgatccgaa
     2101 gcacccggtg ctcgccgagc tcgctggccg actgcaccgc caccacctcg gcgccgtcac
     2161 cttcagccgc ggcatcgagc ctggcgaggt cgctgacctg ttgcgcaccc tggccgtgga
     2221 ggcggaccgc accggtcagc cgctcggcat gggcgatccg gcgaaactgt cggcctggaa
     2281 acacgcccgg ctccacccca tgacatacga gcggctcgag ctgctcgacc aggcgcccgc
     2341 agtcgagccc gatacggcgg ccgaggcccg ctcgggcgat gaagccacgt tgcgcgacgc
     2401 gcgggtccgt ggagcccaac tgtggatcgg cctggcccgg gcggcactgg cgggccagat
     2461 cggcgaggac gaagcgccgc cgacgcgacc cgccgtgatc gccaaggcga tcgacgagca
     2521 cgccggcggc gaggcctacg atcaggtgat cgtcggctac ctgctgcaga tcgcccagga
     2581 gctgaaggcg gcgggcgggc aggaggccct ggagctgcgg cggcgcacgt cgcggctgct
     2641 gtccgagatg cagcccgaaa cgctgcgccg cctgatccag atggggggag atacggcgca
     2701 gcggcggcag ttcgtcctcg atgcctcgca cggcatggcc gtggatgccg tgctcgaagt
     2761 cgtcaaagcg gcggccgatg cctcccagca cgccgtgtcc tcctccctga tgcgcatgct
     2821 gtccaagctc gcgacgcacg ccgagcaggg gaccacccag gcccggtcgc tcgcggacca
     2881 cgagctgcgc gatcaggtcc ggtcgctgct caccgggtgg gagctggagg accccaatcc
     2941 ggcggcatac ggcgcggcgc tgcagcggct gtcgcaggcg cggccgacca tggtggcacc
     3001 gacggagcgg atgcacgccg ccgagccgga gcggctcgtg cagatgagcc tcgaggtgaa
     3061 tgcggtgggc ccgcacgtct atcgggcctg cgctgagctg gtggacagcg gccgtctccc
     3121 gctgctgatc gatctgctgg agcaggcgcc cggcacggcg gcggcggagg agctctggaa
     3181 ccgggcggtg agcccggata ccgtgcgctg gttgctccaa catgagccgg cggacttcgt
     3241 cacggtcgat cgcgtgctgc cacggctcgc gcagcaggcc gccgagccgc tgctcgacgc
     3301 gctcgcggac tccgaggtgc gcgcgacccg ccgggcgatt ctcgagcgcc tggtgcggct
     3361 cgggcccgtt ctgggccaga gcattaccgc gcgcctgagc gacgagcggt ggtacgtcaa
     3421 gcgcaatctg ctgaccctgc tcgatctcct gcccgagctg ccgccggggt tctcggcgct
     3481 gcagtggctg ggcgactccg atccgcgcgt gcgcgtcgag gcgctgaaac tgggtctcaa
     3541 gtctccggcg gagcgcgacc gggtgatcac gcgcgggctc caagacacgg agcatcggat
     3601 cgtacggctc gcgttgctgg cagcgtttca aggggtgccg gcggacgcgg tgccgcagct
     3661 ggtcgccctg gtgaacaacg ggaccctgcc gcccgacatg cgggttccgg cgatccgcct
     3721 gctcggggca gcacggtcgc cgcaagccct ggagacgctg ctcgcgctgg ccgacggcgg
     3781 gcgcacgctg ctggggaggc gcaggctgcc gcccaaatca cgggagctgc tgacggcact
     3841 cagggcgccc gcgagcggct ggaggacgga gccccgcgcg atggaagtgc tgctggtcgc
     3901 ggcggtatcg agcgacgccc agatccgggc ggccactgat ccggccggag cggccgtgtg
     3961 agcgagcccg ctgagtttct cggcgccttc gcgcaagccc tggccggcat gatgctctac
     4021 gccgagggcc acccggcacg cgcgcgcggt atcgacgcag cgtatgaaga gctgctcgat
     4081 ctgcagctgg agacgccgcg ccccctgttc acgttcctcg gggacgaggt catcttcggg
     4141 cgcctgccgc tcagggagat gcgcagctgg gactggtcga tgcggctggc cgaggcgggg
     4201 atccagcgac tcgaattcat ggagcgcgta tcgcgcgaag acttcgaggc cttcctcgag
     4261 gacgtcctgg cacgcgtcac catgacgccg atcgacacgg cggaagcgcg tcaactgcgg
     4321 cccacctcca tcaagttcgg cgccgtgggc gttcgcagcg gggcggggga cctgccgcag
     4381 gaggaggcgt taacgaccgc caccatcgcc ttctccctgg gcgaggaagc ggagaccatc
     4441 cgctggctgc accgcgaggt gcaggagcgc gaggcccttc ccctggcgga ggcagaggcc
     4501 gtggtgcgct cgttgtcggt ggcgatgcac ggcgaccaac agatgatgct gcccctcctc
     4561 cagctcaaac agttcgatca gtacacgacg acgcactccc tgaacgtcgc ggtgctcagc
     4621 atggcgctcg ccgagttcct ggggatgggg gccgccgacg tgcgcgcgtt cggcgtggcg
     4681 ggattgctgc acgacctggg caaggtgcgg atcccgatcg acgtgctcac caaacccggc
     4741 aagcttacgc cggacgagcg cgtgttgatg aacacgcacc ccgccgaggg cgcccgcatc
     4801 attctcgaga gcgagcagga gctcgacctc gcggcggtgg tggcgtatga gcaccacatc
     4861 atgttgaacg gcctgggcta tccccaggtg aagttccggc ggccgtgcca cgaggcgagc
     4921 aacctgatcc acgtctgcga tgtctacgac gcgctgcgga ccaggcggcc ctatcgggag
     4981 gcgtggtcgg tggagcaggt attgccctac atcgaggggc gggctgggct cgaattcgat
     5041 ccggcctacg tgcgggcctt catcgcgatg atgcggaaat gggattcgca ggtcgccgtc
     5101 gtctccgacg agcgcgcccc gcttccggcg tccgcggccg ccgcggagac gggctccgcc
     5161 ggtcccgaag cgagcccctc gagccatcct gtttgattaa gttccgggca cgatggacaa
     5221 gaccagtctg ctgctcgcga tgtgtacttt gggcgctacc gggctgtccg cgcagcgggc
     5281 gaccgtacca cggctggagg gaaagtggga agcccgcacc gacgacggcc cgcgtctcat
     5341 catggtgcgc gccgacagct ccgcccagtt cggcgatcag gtggcgcgct ggcgtatggt
     5401 gggagacagc gtgtggttca cgctgggtga tggagtctgg aacgtctacg gcctgaaggt
     5461 cgcgggcgac aagctcacca tctcgggggg cgatctcgag aagccggtga cgctgcgccg
     5521 cgtgggacca cccacccccc ggcccgacac gctcaaggtc ccggagccgc cgcctgccag
     5581 cgcgcgggcg tggtagaggc agggagtggg gagtggcgac cagctgagcc gatcttcagt
     5641 gcgacccgtc aggacccact ccctacgccc cactccccgt catttcgtcg accagtcgta
     5701 gatccttccc agcaccgccc gcagctcatc cgtcacttcg acgtcgcgcc gcggcagcac
     5761 ccgctccacg atccgcagga acccctcgcg ggtcatgcgc tcgcgcgagg cgccgcccca
     5821 ggcgaacggg gcgacgtatt tgggcgggcg caccgcgtcg aacacgttgg cccccgcacc
     5881 gaccaccgag ccggtgccga ggagcgtgcc gatcgcggtc ttcgcgtggt ccccgaacag
     5941 ggtgccgagg tattgctgcg ccgtgtccac gcgggcaccg ggaatctcga gccggacggg
     6001 tccgtaggtg ttcttgagat tcgaggtgat ggtcccggcc ccgcagttga cccagcgtcc
     6061 cacgacgctg tgacccagga agccgtcgtg ggacttgttg gcgtagccca ggaacacgca
     6121 cgacgccacc tcgccgtgaa cggtgcagcg cggaccgacg gccgagccac ggagatcgcc
     6181 gccgagcacg cgcgcattgg cgccgaccca gagcgggccc tcgagacggg tccccgagcg
     6241 gatctccacg ccgctctcca ccaccaccgg gccgtgcgac acgtcgaaca ccacgcccgg
     6301 ctcgacctgc gcctcgtgca gctccagggc cgcgggatcg ccgatgagga tggaccccgc
     6361 gggcaccgga tcgctgtcgc ccagcatctg ccgcaggtcg tcggggagga gctgcagcag
     6421 cgccgggatg aggtcgaacg tcccgtgcag caccaccccc gggacctcga tggcctgcgc
     6481 ctgcggctgc ggcccctccc acacgacgcc cgccggtacc ccccacccga ccgtgatgcc
     6541 gtgcgacacg agccgcaccg gtacgtgggg cagctcgggc gcgacgccct gtggtgcgaa
     6601 ggtcgacgat ccgatcaccg ccggtccggt aacggcgcgc cgcggcatga ccggcggtgt
     6661 gccgggctcg gcgaaccccg ccagatggga cagcgcgaag acggcgtcgg cctcggcgcc
     6721 cacgaacgtc tcccaccgct cgcggatgag atgagcgccg gcgcgcagct ccgacagcgg
     6781 gcgcacgccg gcgaacggcg cccacaccgg cgcggggtcg gggtcgagca ggtagagagc
     6841 gaggctcata gccgctgcac cagcgtcttg agccggtcgg cgcggccggt cggcatcacc
     6901 agagtgtgcc cgttggcgcg taccaccacg aggtcggtga tcccgctgac cgcgagatgc
     6961 tcgctctccg accagagcac acaacgccgc acatcgtcgc cgatcgtgat gttgccgacc
     7021 gccacgttcc cctgggaatc gggcgtgcgg atgcggaaca gcgcatccca ggagccgatg
     7081 tcgtcccaga cgaaggtccc cgccacgacc gccatccgtt tcgaccgctc caggacggcg
     7141 atgtccaccg gggccggggt cacggccgcg aagaaccccg cgacgtcgcc ggagctcaag
     7201 gccggcagtc cggctcgcag ctcgcgcgca tgggcggagg cctccgccag aaaccgctcc
     7261 acgccccagg cgaacagtcc cgtgttccac agcgccttgc gccgccgcag ctcggcggcc
     7321 cgcgccgcgg tcggcttctc gacgaaccgc tgcacgcgtc gggcgagccg tccgaggggg
     7381 cgtcccggga cgatatagcc gtaggccgac tcggggcgcg tcggcttcac gcccaccgtc
     7441 accagcacat cgtgctcgcg cgccacggcg agcgcccgcg cggcggtggt gcggaacgcc
     7501 gcctcgtcgc cgatggccca gtcggcatgg agggagagca tctgcgcgtc cgggtcgcgc
     7561 cgggcgatcc agtgcgcgcc ccacaccagc gcgggggcgg tcgacgcgga ccggggctcc
     7621 gccagcacct gcgcccttgg cacttcgggc acgagttccg tcacccgctc cacgagaaag
     7681 gggccggtga ggatcagcac gcgatccggc ggcaccagcc cttcgagccg gcgcacggcc
     7741 tgagaaagga gcggacggtc gccggcgagc ggcagg
//
LOCUS       DBNGZW010000032         3025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_958148, whole genome shotgun sequence.
ACCESSION   DBNGZW010000032 DBNGZW010000000
VERSION     DBNGZW010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3025
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_958148"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="AC16JC_00760"
     CDS             complement(<1..728)
                     /locus_tag="AC16JC_00760"
                     /inference="COORDINATES: protein motif:HMM:NF015644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HIR5163254.1"
                     /translation="MTARPGVLRVRAPRPRRRRLLVLLLVIFLLVLFLPSLVTLAAEW
                     PWFTALGYARVFATRLVAKGVLGIGAGAFAFAFLYANLRFAQRGVAPDQVFLRFNEGT
                     AGLDVMGLLRRLVLPTALGVALLFGLGASAGWLGVLQFLHRTPFGVTDPVFGRDVGYY
                     VFTVPVVSSVLGFLTALTLIALLGSIALYVLRRDVVLAGRRVTVEPSARWHIAGLIAF
                     LFLLAALRVYFARIPGLLYSTTGPL"
     gene            complement(738..1910)
                     /locus_tag="AC16JC_00765"
     CDS             complement(738..1910)
                     /locus_tag="AC16JC_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011390788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR5163255.1"
                     /translation="MICQRHLFDIPEDVAYLNCAYMSPLLRRVTAAGQRAVALKAHPW
                     DITPADFFSEVDVARRRFAELLGDGAGEDDIAIVPAVSYGMAVACANLPLTRGQTVLL
                     LDEEFPSVILPWRERAREAGATAVLLPRPADDDWTRVVLEAIDERTAVAALPALHWTD
                     GALIDLPRVATRLREVGAALVVDATQSLGAMPFPLGAVRPDFLVAAAYKWLLGPYSLG
                     FLYVAPQHQHGRPLEYNWITQTGSEDFTALTNYAREPELQPGARRFDVGDTSNFALMP
                     MAIAALDQILEWGVPHIAETVAALTSRLVARAATLGLQAVPAARRAPHYVGLRFPGGV
                     PSELPQRLAAARVYASVRSRGALRVSPHVYNTEADIDRLIDAIAAATKTAVTGAEG"
     gene            complement(1907..>3025)
                     /locus_tag="AC16JC_00770"
     CDS             complement(1907..>3025)
                     /locus_tag="AC16JC_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163256.1"
                     /translation="GPVPLESSDSANLLVGSYPRYVSSGHLLYLVGNTLMALPFDPEH
                     LRVLGPPAPVIQELRRESWDAAGQYAIAADGTLLFARGADASKSVPVWVDHTGRVTDT
                     LPVPPGDYFNIFASNNGKRIEFSTYQHSGEQTASILDVDRGLTQTLHFAELVTFTSWW
                     PDGEHANLYLGPSAALERGITWRVPISGTGNRDSLLGPGWMVRDVSRDLRYLSVNRFG
                     DSAGVWLVSTDGKQRQFIDPSGWWSVFSPDSRWLAFPSNEGLKISAVPPTGSLQTVAP
                     ASADEPEWSPHGDELYYRDGKRWMAMAVSTRGGLTVGRPRLLFEGRYLNVWSKSYDVG
                     PDGRFLLLLGPPEETVGHLDVVTGFFAELRRLAPAGIK"
BASE COUNT          560 a         1021 c         1021 g          423 t
ORIGIN      
        1 agcggtcccg tcgtcgagta cagcagtccc gggatgcgcg cgaagtacac ccgcaacgcg
       61 gccagcagga agaggaaggc gatcaacccg gcgatgtgcc accgcgctga gggctccacc
      121 gtgacgcggc gccccgccag gacgacgtcc cggcgcagca cgtacagcgc gatgctcccg
      181 agcagcgcga tcagcgtcag cgccgtgagg aagccgagga cgctgctcac gaccggcacc
      241 gtgaacacgt agtagccgac atcgcggccg aagaccgggt ccgtgacgcc gaatggcgtc
      301 cggtgcagga actggagcac gccgagccag ccggccgagg cccccaggcc gaacagcagt
      361 gctacgccga gggcggtggg gagaaccagc cgacgcaaca gccccatcac gtcgaggccg
      421 gcggtcccct cgttgaagcg gaggaacacc tggtccggag cgacgccgcg ctgggcgaac
      481 cgcaggttgg catagaggaa ggcgaacgcg aacgcaccgg ccccgatacc gagcaccccc
      541 ttggccacga gccgggtcgc gaacacgcga gcgtagccga gggccgtgaa ccagggccac
      601 tctgcggcaa gggtgaccag actgggcaga aacaggacga ggaggaagat cacgagaagc
      661 aggaccagca ggcggcgacg gcgcggccgt ggagctctca cgcgcagtac gcccgggcgg
      721 gcagtcatgc gagtaagcta accctccgcg cccgtcacgg ctgtcttcgt agcggccgcg
      781 atcgcgtcga tcaagcggtc gatgtcggct tcggtgttgt agacatgggg agacacgcgc
      841 agcgcgccgc gactgcgcac gctcgcgtac actcgggctg ccgccagccg ttgcggcagc
      901 tcggacggca ccccgcccgg aaaccggagg cccacatagt ggggcgcgcg tcgcgcggcc
      961 ggcaccgcct ggagaccgag cgttgcggcg cgcgccacca gccggctggt cagcgcggcg
     1021 accgtctcgg caatgtgcgg caccccccat tccagaatct ggtccagcgc ggcgatcgcc
     1081 atgggcatca gcgcaaagtt cgaggtatcc ccgacgtcga agcgtcgcgc ccccggttgc
     1141 agctccggct cccgggcgta gttggtgagc gcggtgaagt cctccgagcc cgtctgcgtg
     1201 atccagttgt actcgagcgg cctgccatgc tggtgttgcg gggcgacgta gagaaacccc
     1261 aggctgtacg gcccgagcag ccatttgtag gctgccgcga cgagaaaatc gggccgcacc
     1321 gcgcccaggg gaaacggcat ggcgccgagt gactgggtcg cgtccaccac caacgcggcg
     1381 ccgacttcgc gcagccgtgt cgccacccgc gggagatcga tgagcgcgcc atcggtccag
     1441 tggagcgcgg ggagcgcggc gaccgcggtg cgctcgtcga tcgcctcgag gacgacccgg
     1501 gtccagtcgt cgtcggcggg ccgcggcagc agcaccgcgg tggcccccgc ctcccgtgcc
     1561 cgctcccgcc agggcaggat cacggaggga aactcctcgt ccagcagcag cacagtctgg
     1621 ccgcgcgtga gcggcaggtt ggcgcaggcg accgccatgc cgtagctcac cgccggcacg
     1681 atcgcgatgt cgtcctcgcc ggcgccgtcg ccgagcagct cggcaaagcg tcggcgcgcc
     1741 acatcgacct cggagaagaa atccgccggt gtgatgtccc aggggtgggc cttgagcgcc
     1801 acggctcgtt gtccggccgc agtgacccgg cgcaacaacg gcgacatgta ggcgcagttc
     1861 aggtacgcga cgtcctcggg gatgtcgaac aggtggcgct gacagatcac tttattccag
     1921 ccggggcgag ccgtcgcaat tcggcaaaga aaccggtcac cacgtcgagg tgtcctacgg
     1981 tttcctccgg tggcccgagg agcaggagga atcgaccatc cgggcccacg tcgtagctct
     2041 tcgaccacac attcaggtac cggccctcga atagtagccg tggtctccca accgtcagtc
     2101 caccccgagt cgacacggcc atggccatcc agcgcttgcc gtcgcgatag tacagctcgt
     2161 cgccatgggg tgaccactcc ggctcgtcgg cgctcgccgg agccactgtc tggaggctgc
     2221 cggtgggtgg aactgccgag atcttcaacc cctcgttgga tggaaaggcg agccagcggc
     2281 tgtcgggtga aaagaccgac caccagccac tcgggtcaat gaactgtcgc tgcttcccgt
     2341 ccgtagaaac cagccagact ccggcggagt caccgaatcg attgacgctc agataccgaa
     2401 gatccctgga gacgtccctg accatccagc cgggtccgag caggctgtcc cgattccccg
     2461 tcccgctgat cggcactcgc cacgtgatgc cccgctccag cgccgcactc ggccccaggt
     2521 agagatttgc atgctcccca tcgggccacc aggaggtgaa ggtcaccaac tcagcgaaat
     2581 gcagcgtttg agtcaggccc ctgtccacat cgaggatcga ggcggtctgt tctccgctgt
     2641 gctggtaggt agaaaactct atgcgcttac cgttatttga cgcaaagatg ttgaaatagt
     2701 cacccggtgg caccgggagg gtgtcggtga ctcgtcccgt gtggtcgacc cacacgggta
     2761 cgctcttcga cgcgtcggcg ccgcgggcga agagcagcgt gccgtcagcc gcgatggcat
     2821 actgtccagc ggcatcccag ctctcccgac gaagctcctg aatgaccggt gccggtgggc
     2881 ccagcacccg caagtgctcc gggtcgaagg gcagggccat gagggtattg ccaacgagat
     2941 aaagcagatg cccgctgctg acataccggg gatagctccc aaccagcaag ttggcggaat
     3001 cggacgattc caaggggacc ggacc
//
LOCUS       DBNGZW010000033         8046 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_139665, whole genome shotgun sequence.
ACCESSION   DBNGZW010000033 DBNGZW010000000
VERSION     DBNGZW010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8046
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_139665"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="AC16JC_00775"
     CDS             <1..525
                     /locus_tag="AC16JC_00775"
                     /inference="COORDINATES: protein motif:HMM:NF003995.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HIR5163257.1"
                     /translation="AELARRGGTTSAQVRKDLSFFGSFGKRGLGYLVPELSARIREIL
                     GLKRTHRVVLVGAGKMGGALLHYRGFRQHGFDVVAIYDKDPKKIGTRSDGLMVRDVRQ
                     LEADLKKEPPDIAILVTPAEAAQEVTDRLVRAGVKAILNFAPVSLAVPADVAVQSVNM
                     ALELETLSYALANR"
     gene            651..1883
                     /locus_tag="AC16JC_00780"
     CDS             651..1883
                     /locus_tag="AC16JC_00780"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF014344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016990 - arginine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0000050 - urea cycle [Evidence IEA];
                     GO_process: GO:0006527 - L-arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HIR5163258.1"
                     /translation="MPRVHVTSEIGPLRHVLVHTPGNELTAVTPGNREDYLYDDIIDL
                     ELAQREHRRLVAVLERFADVYEVRDLLTEIVARSDVRDFLVTRALEVVPSDALAKQLA
                     VLSAEDLVTLMIEGTHEDAGPIARALNEVGYALPPLPNLFFTRDVGIVIGEHAVIGSM
                     RYGVRWTEEMLIKALFRYHEKLENAGILYDGSEERRHTYTLEGGDVHPLRPDLLVLGF
                     SERSSPAALDQLCELAFEKCGVTDVIVVVLPNERTAIHLDMIFTQIDRESCVVYLPFF
                     VGPERLAVLHRRRRSKGVKEIPNFFAAMQAVDLPLEPVFCGGNARSVQEREQWSSACN
                     FFTIRPGVVITYARNEATLRELEHAGYRIVTAVNLLTGEEWLEEGDRGVITIEGSELV
                     RGGGGPRCMTLPLRRDDL"
     gene            2000..2863
                     /locus_tag="AC16JC_00785"
     CDS             2000..2863
                     /locus_tag="AC16JC_00785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5163259.1"
                     /translation="MPHSTFSVGHLRCHLLETGLQHLDGGAMFGVVPKPLWERRISPD
                     AKNRIPLALRCLLVEHDDGLVLVDTGIGDKDDAKFREIYGVDNLAADGRTRLAHALEE
                     LRFGPEDVRWVVNTHLHFDHAGGDTRRAPSGEVLPAFGRARYVVQRGDLEFARHPNER
                     TAGSYLAANFAGIDFRLLDGEVEVLPGIRCLPTPGHVPFHQSVLIESGGERACFLADL
                     VPTSAHLPLPWIMGYDVEPLVTLETRRWLYRRAEAEAWRLCFEHDPVVASGRLVRDGK
                     GFGLVDTVAAA"
     gene            3074..3610
                     /gene="infC"
                     /locus_tag="AC16JC_00790"
     CDS             3074..3610
                     /gene="infC"
                     /locus_tag="AC16JC_00790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HIR5163260.1"
                     /translation="MPAPESSVPSKQPRTRINRMIRISPVRVIADDGAQLGVLSIDEA
                     LAAAQERGLDLVEVAPMARPPVVKIMDYGKFKFEQAKAARAAKKKQHVIHLKEVKYRP
                     GIDEHDFAFKTRHAREFLESGNKVKVTMMYRGRQMAHIELGREVLDRVATELKDIGKI
                     EAEPRLEGRNMSMVLAPK"
     gene            3707..3892
                     /gene="rpmI"
                     /locus_tag="AC16JC_00795"
     CDS             3707..3892
                     /gene="rpmI"
                     /locus_tag="AC16JC_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005364784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HIR5163261.1"
                     /translation="MKSKRALRKRVRLTGSGRLRRHRAMKSHILTKKHPKRKRHLRKG
                     TLVAHADERRLKRLLSA"
     gene            3903..4256
                     /gene="rplT"
                     /locus_tag="AC16JC_00800"
     CDS             3903..4256
                     /gene="rplT"
                     /locus_tag="AC16JC_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011989604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HIR5163262.1"
                     /translation="MPRVKSNVARLKRKNKVMKLAKGYFGGRSKLWKAAKESVERAQK
                     YAYRDRRRKKGEFRRLWITRINAAARLHDLSYNRLMNGLRKAGVEINRKVLADLAVRD
                     PGAFEQLAEVAKKAL"
     gene            4259..5287
                     /gene="pheS"
                     /locus_tag="AC16JC_00805"
     CDS             4259..5287
                     /gene="pheS"
                     /locus_tag="AC16JC_00805"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015864754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR5163263.1"
                     /translation="MRDLLARLDGLRERLREIPQADERRLQELRNEILGRKNGALTDI
                     LKALPALDADTRKRVGGAANTLKREFEEAFEARAAALALADRALGDVDPTMPARAQWR
                     GGLHVVTQVVHEICDTFRELGFTRVVGPEIETERNNFYALNFPHDHPALDSQDSFYVT
                     DDLLLRTHTSPVQIRTMEAYRPPIRVVIPGQVYRRDAFDASHAPVFEQIEGLVVDEGI
                     SFVDLKATLSLFARRLFTADTVVRFHPSYFPFTEPSADMSVRCTVCGGSGCPTCKHSG
                     WMEILGSGMVHPAVLENCGIDPERYTGFAFGMGPARVAMLRYGIADIRLFFESDVRFL
                     AQFARGIR"
     gene            5284..7668
                     /gene="pheT"
                     /locus_tag="AC16JC_00810"
     CDS             5284..7668
                     /gene="pheT"
                     /locus_tag="AC16JC_00810"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR5163264.1"
                     /translation="MNLSRRWLEALLGRPLDAAQVEQILTAHCCPVEGIEPIHGDLAE
                     VLIARVLEVKAHPNADRLRLCLVDAGSTDGPLEVVCGAPNVEAGKHYPFAPVGANLPG
                     GVKLERRKIRGIASNGMLCSARELGLGQDHEGILELQTDAAPGTRFLDAVPIGDDRIL
                     LEVYANRPDLLGHKGVARELAAALGGTVKLPAFPGAPSQQSAYPPIRRSAQTGVVDGV
                     EVRLEDSEGAPRYMIAVIRGVRVGPSPAWLADRLTAIGQRSINNVVDATNYILFELNQ
                     PMHAFDLARLRGPAAVVRRARPNEKIVTLDGVERTLTPDMTAICDAERPTIVAGVMGS
                     AESEVAETTTDLVLECACFQPTRIRRTRRALGLSSESSYRYERGIDLQGMPDALRRAI
                     DLIVAVAGGTVREAPLDLWPEPQQPRSVFLRPERAERLLGVAVPRPELERLLASVGFV
                     VAPKDGRVAVQVPGWRPDVTAEVDLIEEIARLRGYDAFPDELRPYRPGTVPDAPSQQV
                     LHRLREQVARAGFLEARTSPLGPAEGADAVALANPLSAAESYLRANLLPGLVRRVEYN
                     WSVRERDIRLFEVGTVFRAGAPPEERLALAGVLTGARRPPHWADGGDAKQPDMDIWDL
                     KHHFELAVSEAAPGARVVPGTGEELWRAVAGTDGRQVGRAARLAADAPRWAGALFGFE
                     VEVDVAAERAVHFAPLPAQPPTLQDLSLVLPGGVTAEQVEGLMRQAGGVLLERLTVLD
                     EYRGPKFPAGTRGVTWRCVFRDPSRTLRDSEVDDVIRRALALLEGELGVRRRAG"
     gene            7646..7990
                     /locus_tag="AC16JC_00815"
     CDS             7646..7990
                     /locus_tag="AC16JC_00815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163265.1"
                     /translation="MYDAERADVLALEQLEAVLREVAEELATWRARALKAEGHRGGGG
                     GGGGGRGDGDGRGRAAELEAENRQLRTRVDAARNRVQELLARLAFLEEQSREAAAAGG
                     ANGGGSGRGATR"
BASE COUNT         1276 a         2634 c         2889 g         1247 t
ORIGIN      
        1 gccgagctcg cccgccgcgg cggcaccacg tcggcgcagg tccgcaagga cctctccttc
       61 ttcggctcgt tcggcaagcg ggggttgggg tacttggtgc cggagctctc ggcgcggatc
      121 cgcgagattc tcgggctcaa gcggacgcac cgggtggtgc tggtgggggc ggggaagatg
      181 gggggcgcgc tgctgcatta ccgcggcttc cgccagcacg gcttcgacgt ggtcgccatc
      241 tacgacaagg atccgaagaa gatcgggacg cgctccgacg ggctcatggt gcgcgacgtg
      301 cgccagctcg aagctgatct gaagaaggag cctcccgaca tcgccatcct cgtgacgccg
      361 gcggaggcgg cgcaggaagt caccgaccgg ctggttcgcg ccggggtgaa ggcgatcctc
      421 aacttcgctc cggtgtcgct cgccgtgccc gccgacgtcg cggtgcagag cgtgaacatg
      481 gcgctggagc tggaaacgct gagctacgcc ctggcgaacc gctaggcgat tgcggattgc
      541 gaattgagga ttgcggattg actgcgcccc ctttcctaag gtcagtcttg ctgctgccaa
      601 tccgcaatcc gaaatccgca atccgcaatc gcagttagat ttcccgccct atgccccgcg
      661 tccacgtcac ctccgagatc ggtccgctgc gtcatgtgct ggtgcacacg ccggggaacg
      721 agctcacggc ggtcacgccc gggaatcggg aagactacct gtacgacgac atcatcgacc
      781 tcgagctcgc gcagcgggag caccggcgcc tggtagcggt gctggagcgg ttcgccgacg
      841 tctacgaagt gcgggatctg ctgaccgaaa tcgtggcgcg ttccgatgtg cgcgacttcc
      901 tggtcacgcg ggcgctcgag gtcgtgccgt ccgacgcact cgccaagcag ctcgccgtgc
      961 tctcggccga ggacctggtc accctgatga tcgaggggac ccacgaggat gccggtccca
     1021 tcgcgcgggc gctcaacgaa gtcggctacg cgctgccgcc gctccccaac ctcttcttca
     1081 cccgcgacgt ggggatcgtc atcggggagc acgcggtgat cgggtcgatg cgctacggcg
     1141 tccggtggac cgaggagatg ctgatcaagg ccctgttccg ctaccacgag aaactggaga
     1201 acgccggcat cctctacgac ggctccgaag agcggcggca cacctacacc ctcgagggcg
     1261 gggacgtgca cccgctgcgc cccgacctgc tggtgctcgg gttcagcgaa cgctccagtc
     1321 cggctgcact cgatcagctg tgcgagctgg ccttcgagaa gtgcggcgtg accgacgtga
     1381 tcgtggtcgt gctgcccaac gagcgcacgg ccatccatct cgacatgatc ttcacccaga
     1441 tcgatcgcga gagctgcgtg gtctacctgc cgttcttcgt gggcccggag cggctcgccg
     1501 tgctgcaccg gcgtcgccgt tccaagggcg tcaaggagat tcccaacttc ttcgccgcca
     1561 tgcaggcggt ggacctgccg ctcgagcccg tgttctgcgg cggcaacgcg cggtcggtgc
     1621 aggagcgcga gcagtggtcc tccgcgtgca atttcttcac cattcggccc ggtgtggtca
     1681 tcacctacgc ccgaaacgaa gccaccctgc gcgagctgga gcacgccggg tatcgcatcg
     1741 tcaccgccgt gaacctgctg acgggcgagg aatggctgga agaaggcgac cggggcgtga
     1801 tcacgatcga gggcagcgag ctggtgcgcg gcgggggagg accgcgatgc atgacgctgc
     1861 cgctgcggag agacgatctg tgagtcaatt gcggattgtc gattgcggat tgcggattga
     1921 caggtcgagt ctcgctctcc cggagcacga gccacccatt ccaatccgca atccacaatc
     1981 cgcaatccgc aattagtcga tgccgcactc cacgttttcc gtcggtcacc tccgctgcca
     2041 cctcctcgaa accggcctcc agcacctgga cggcggggcg atgttcgggg tcgtgccgaa
     2101 gccgctgtgg gagcggcgta tctcccccga tgcaaagaac cggatccccc tggccctgcg
     2161 ctgtctgctc gtggagcacg acgacggcct ggtgctcgtg gacaccggca tcggcgacaa
     2221 ggacgacgcc aagttccggg agatctacgg cgtcgacaac ctcgccgcgg acggccgtac
     2281 gaggctggcg cacgcgctgg aggagctgcg gttcggcccc gaggacgtgc gctgggtcgt
     2341 caacacccac ctgcatttcg accatgcggg aggcgacacg cgccgggctc cgtcgggtga
     2401 ggtgctgccc gccttcgggc gggcgcgcta cgtggttcag cggggcgacc tggaattcgc
     2461 ccggcacccc aacgagcgca ccgccggcag ttacctggcc gcgaacttcg ccggcatcga
     2521 cttccggctg ctggacgggg aggtcgaggt gctgcccggg atccgctgtc tgcccacgcc
     2581 cggacacgtg ccgtttcacc agtccgtgct gatcgagagc ggcggcgagc gggcctgctt
     2641 cctcgccgac ctcgtgccga cgtcggccca tctgccgctc ccctggatca tgggctacga
     2701 cgtggagccc ctggtcaccc tggagaccag gcgctggctg taccggcggg ccgaagccga
     2761 ggcctggcgg ctctgcttcg agcacgaccc ggtggtggcg tcggggcggt tggtgcgtga
     2821 cgggaagggg ttcggccttg tggacaccgt ggccgcggca tagcttggtg gcgctgtgat
     2881 aagcgggcgc cgcgggcgcc ccacctccag gcctctccag ggtgatcgga gagcgctgcc
     2941 gggttccgag aagcggtcgt cggtcatctg tcctcggttg tcgacaaccg acgactgact
     3001 ccgacgaccc tgcaacgcgg attcccgagc gactggggtc cgcgttttca ttcatgagga
     3061 ggagaaccag cacctgccag cacctgaaag cagcgtaccg agcaaacagc cgcggactcg
     3121 gatcaaccgg atgatccgca tcagcccggt tcgggtgatc gcggatgacg gcgcacagtt
     3181 gggcgtcctg tccatcgatg aggcgctggc ggcggcgcag gaacgcgggt tggacctggt
     3241 ggaagtggct cccatggccc gaccgcccgt ggtcaagatc atggactacg gcaagttcaa
     3301 gttcgagcag gcgaaggccg cgcgtgcggc gaagaagaag cagcacgtga ttcatctgaa
     3361 ggaagtgaaa taccggcccg ggatcgacga gcacgatttc gcgttcaaga cgcggcacgc
     3421 acgcgagttc ctggagtccg ggaacaaggt caaggtgacg atgatgtatc gcggtcggca
     3481 gatggctcac atcgagctgg gccgcgaggt gctcgatcgg gtcgcgaccg agctcaagga
     3541 catcggcaag atcgaggcgg agccgcggct cgagggccgc aacatgtcga tggtgctggc
     3601 gccgaaataa cgggatcagg tgccagggat cgggtgccag cgctcgcgcg atccgacacc
     3661 tgacacccgg aacctgacac ctgaagaggt tcgtgccatg ccgaagatga agagcaagcg
     3721 cgccctgcgc aaacgggtgc gactcaccgg ctcggggcgg ctgcgccgcc accgggcgat
     3781 gaagagtcac atcctcacca agaagcatcc caagcggaag cgtcacctgc gcaaggggac
     3841 gctggtcgcg catgccgacg aacgccgcct gaagcggctc ctctcggcct aggagaccgc
     3901 acatgcctcg ggtcaagagc aacgtcgcgc ggttgaagcg caagaacaag gtcatgaagc
     3961 tcgccaaggg ctatttcggc gggcgcagca aactgtggaa ggccgccaag gagagcgtcg
     4021 agcgggccca gaagtacgcc taccgcgacc gccgccgcaa gaagggcgag ttccggcggc
     4081 tctggatcac gcgcatcaac gccgcggccc ggctgcacga cctgtcgtac aaccgcctca
     4141 tgaacggcct gcgcaaggcc ggtgtggaaa tcaaccgcaa ggtgctcgcc gatctcgcgg
     4201 tccgggatcc cggagcgttt gagcagctgg cggaggtcgc gaagaaggcc ctctgaccat
     4261 gcgcgacctc ctcgcgcgcc tcgacggcct gcgggagcgt ctgcgcgaga ttccccaggc
     4321 cgacgagcgc cggctccagg agctccgcaa cgagatcctg ggtcgcaaga acggcgccct
     4381 cacggacatc ctcaaagccc tcccggccct cgacgcggac acccgcaagc gcgtcggcgg
     4441 ggccgcgaat accctgaagc gcgaattcga ggaagcgttc gaggcgcgcg cggccgcgct
     4501 ggccctggcc gaccgggcgc tgggcgacgt cgatcccacg atgccggcac gcgcgcagtg
     4561 gcgcggcggt ctgcacgtcg tgacgcaggt ggtccacgag atctgcgaca ccttccgcga
     4621 gctggggttc acgcgggtcg tgggtccgga aatcgaaacc gagcgcaaca atttctacgc
     4681 gctcaacttc ccgcacgacc acccggcgct cgattcgcag gactcgttct acgtcaccga
     4741 cgacctgctg ctgcgcacgc atacctcgcc ggtgcagatc cgtaccatgg aagcctaccg
     4801 cccgccgatc cgcgtggtga tcccggggca ggtctatcgc cgggacgcgt tcgacgcgtc
     4861 ccatgcgccg gtgttcgagc agatcgaggg cctcgtggtg gatgagggca tctcgttcgt
     4921 ggacctgaag gcgaccctga gcctgttcgc gcggcgcttg ttcaccgcgg acacggtcgt
     4981 gcggttccac ccctcgtatt ttccgttcac cgagccatcg gccgacatga gcgtccggtg
     5041 taccgtgtgc ggcggctccg ggtgcccgac ctgcaagcac tcgggatgga tggagattct
     5101 gggcagcggg atggtgcatc ccgccgtgct cgagaactgc gggatcgacc cggagcgtta
     5161 caccggtttc gccttcggga tgggacccgc ccgcgtcgcg atgctgcgct acggcatcgc
     5221 cgacatccgc ctgttcttcg agagcgatgt ccggttcctg gcgcagttcg cccgggggat
     5281 ccggtgaacc tctcgcgtcg ctggctggag gcgctgctcg gccggccgct cgacgcggcc
     5341 caggtggagc agatcctcac cgcccattgc tgcccggtgg agggaatcga gccgattcac
     5401 ggggacctgg cggaagtcct gatcgcccgg gtgctcgagg tcaaggccca tcccaacgcc
     5461 gaccggctgc ggctctgcct cgtggacgcc ggcagcacgg acggaccgct cgaagtcgtg
     5521 tgcggggcgc ccaatgtcga ggcggggaag cattatccgt tcgcccccgt gggcgccaac
     5581 ctgccgggcg gcgtgaaact cgagcggcgc aagatccgcg gcatcgcctc gaacggcatg
     5641 ctgtgctcgg cgcgcgagct gggattgggg caggatcacg agggcattct cgagctccag
     5701 accgatgcgg cgccgggcac gcgcttcctc gatgccgtgc ccatcggcga cgaccggatc
     5761 ctgctcgagg tgtacgccaa ccgccccgat ctcctgggcc acaagggggt cgcgcgcgag
     5821 ctggccgcgg ctctgggcgg taccgtgaag ctcccagcat ttccgggcgc tccgagtcaa
     5881 caatccgcct atccgcccat ccgccgatcc gcccagacgg gcgtggtgga cggtgtggaa
     5941 gtccgtctcg aggactccga gggcgcgccg cgctacatga tcgccgtcat ccgcggcgtc
     6001 cgggtgggtc cgagcccggc ctggctggcg gatcggctca ccgcgatcgg acagcgctcc
     6061 atcaacaatg tggtggacgc gaccaactac atcctgttcg agctgaacca gccgatgcac
     6121 gcgttcgatc tcgctcggct gcgcgggccg gcggcggtcg tgcgccgggc tcgcccgaac
     6181 gagaagatcg tcacgctgga cggcgtggaa cgcaccctca cgcccgacat gacggccatc
     6241 tgtgacgcgg agcggccgac gatcgtggcc ggggtgatgg ggagcgccga atcggaggtg
     6301 gccgagacga ccaccgatct cgtgctcgaa tgcgcctgct tccagccgac gcgcatccgc
     6361 cgcacgcgcc gcgccctcgg gctctcgagc gagtcgagct accgctacga gcggggcatc
     6421 gatctgcagg gaatgcccga cgcgctgcgg cgcgccatcg acctcatcgt cgcggtcgcg
     6481 ggggggacgg tgcgggaagc tccgctcgat ctctggcccg agccgcaaca gccgcgcagc
     6541 gtcttcctgc gacccgaacg ggcggagcgg ctgctcgggg tcgcggtgcc gcgtcccgag
     6601 ctcgagcggc tgctcgcgag cgtgggcttc gtggtcgcac cgaaggacgg ccgcgtcgcc
     6661 gtacaggtcc ccgggtggcg gcccgatgtc acggccgagg tcgatctcat cgaggagatc
     6721 gcgcggctga ggggctacga cgcgtttccc gacgagctgc gcccctatcg acccgggacg
     6781 gttcccgacg cgccctcgca gcaggtgctg caccgcctgc gcgagcaggt ggcacgagcg
     6841 ggcttcctcg aggctcgcac ctctcccctc ggccccgcgg agggtgcgga tgccgtggcg
     6901 ctggcgaatc cgctctcggc ggcggagagc tacctgcgcg cgaacctgct cccggggctg
     6961 gtgcgccgtg tggaatacaa ctggagcgtg cgcgagcgcg atatccggct gttcgaggtg
     7021 ggaacggtgt tccgggccgg ggcccctccg gaggagcgtc tggctctggc gggggtgctg
     7081 accggggccc gccggccccc ccactgggcc gacggggggg atgcgaaaca gcctgatatg
     7141 gacatctggg acctcaagca ccactttgag ttagccgttt cggaggcggc ccccggggcc
     7201 cgggtggtac ccggtaccgg ggaggagctg tggcgggcgg tggcgggcac cgatgggcgc
     7261 caggttgggc gggcggcccg gttggcggcg gatgcgccca ggtgggccgg tgcgttgttc
     7321 ggcttcgagg tcgaggtgga cgtggcggcg gagcgtgcgg tgcatttcgc cccgctgccc
     7381 gcgcagccac cgacgctgca ggatctgtcg ctggtgctgc cgggtggcgt cacggccgag
     7441 caggtggagg ggctgatgcg ccaggccggc ggcgtcctgc tcgagcggct gacggtgctg
     7501 gacgagtacc gcgggcccaa atttccggcg ggtacgcgcg gggtgacgtg gcgctgcgtg
     7561 ttccgggatc cgtcgcgcac gttgcgcgac agtgaagtgg acgatgtgat ccggcgtgcg
     7621 ctggccctat tggagggcga actgggtgta cgacgcagag cgggctgacg tgctggcgtt
     7681 ggagcagctc gaggccgtgc tgcgcgaggt tgccgaggag ctggcgacct ggcgcgctcg
     7741 cgcgttgaag gcggaggggc accggggtgg gggaggtggc gggggtggcg ggcgcgggga
     7801 cggtgacgga cgtgggcgcg ccgccgaact ggaagcggag aaccggcagc tgcgcacccg
     7861 cgtggacgcg gcgcgcaacc gggtccagga gctgttggcg cgactggcgt tcctcgaaga
     7921 gcaatcgcgc gaagctgcgg ccgccggagg cgcgaacgga ggcggcagcg ggcgaggagc
     7981 cacgcggtga gcaccaagaa gcacgcggtg cgcgtggtga tcggcggcga agagtacacg
     8041 gtgcgc
//
LOCUS       DBNGZW010000034         3695 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_821438, whole genome shotgun sequence.
ACCESSION   DBNGZW010000034 DBNGZW010000000
VERSION     DBNGZW010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3695)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3695)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3695
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_821438"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..615)
                     /locus_tag="AC16JC_00820"
     CDS             complement(<1..615)
                     /locus_tag="AC16JC_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR5163266.1"
                     /translation="MTAATAPTDFRTCPRTNLKIHAAADSLIRANAVAATVALLIGGI
                     AAILVLLTRWQAVHLLPADWFYRILTIHGMSMLIFFIIFFEMAILYFASAVVLNSRVA
                     TPRLGWAAFVLMVIGAVMTEATMWTGHADVLFTSYVPLQATSWFYLGVILFAVGALVV
                     VFIFFGTLVVAKRERTYQGSVPLVTFGALTAAIIAFLTLAHGAAI"
     gene            complement(612..1184)
                     /locus_tag="AC16JC_00825"
     CDS             complement(612..1184)
                     /locus_tag="AC16JC_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5163267.1"
                     /translation="MHTGLDSPPRVWWLPLHGAERIWVTVAFAWCLVLFAMMPLWHLK
                     GQQNPSGIRHKVEPTRYYQRVQEFIKQYQVGEEAGIPVVAPPPGSDVYLLAQMWKWTP
                     ILKLQKGVEYTLHLSALDVNHGFNLNPVNINFQVVPGYDYGLKVVPNAAGDFRIVCDE
                     FCGIGHHTMVGKVIIVDSAAAPVAAPGGAP"
     gene            complement(1202..1372)
                     /locus_tag="AC16JC_00830"
     CDS             complement(1202..1372)
                     /locus_tag="AC16JC_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163268.1"
                     /translation="MAEQPETRRDDGDIPLIQRLLDRPFLLLAAGIVIMVLFYTAWGL
                     YEILSLKPAPLP"
     gene            complement(1414..1827)
                     /locus_tag="AC16JC_00835"
     CDS             complement(1414..1827)
                     /locus_tag="AC16JC_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RrF2 family transcriptional regulator"
                     /protein_id="HIR5163269.1"
                     /translation="MLSQTAEYALRAVLYVAEHGTDRLVQVTEMATALRIPRNYLSKI
                     VHALVREGVLLSTRGKSGGFRLAVPADRLYVVQVVAPFGSLGEERHCLLGRAQCSDRT
                     ACAAHTKWKLVADRVAEFFRETTIAELLQGAAVPA"
     gene            1889..3472
                     /locus_tag="AC16JC_00840"
     CDS             1889..3472
                     /locus_tag="AC16JC_00840"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR5163270.1"
                     /translation="MAPVAALTKIVRRGFARADTVANRLYGWRYNPLYHSGALVLMLF
                     VVLLVTGVYLLFVYHISEPYESVARITQQAWLGRWMRGVHRYASDAAVVAALFHALRM
                     LVQGRSWGPRTLAWISGLVLLASVFVCGWTGFVMVWDVQGQVMAQAGARIFDLLPIFG
                     EPISRAFTGERALPGAFFFLNLFAHVALPIALFILVWVHVSRLARPGLFPPRWLTWAV
                     VGALLALAVLWPVAMTPPADAFMLPGRAAYDWFYAFWLPLARAVSPVAVWGIGLAIVT
                     AGLAIPWLTRPRLSRRPPPSVVDEKLCTGCEQCYLDCPYEAIAMVDRADAVATGKSAT
                     VARVDPTLCVSCGLCAGSCAPMGVGPPGRTGRAQLTVVRDFIAAHRPGGGDVVVVACE
                     RGAGGATAAAAFDGALVYPVSCGGNLHTSVVEYLLRAGAGGVLIVSCPPRDCWNREGP
                     KWLEARLFHDREAELRERVDRRRVRLVHAAAGERAVVRAALAGFRADLTALEAATVES
                     DIDLDRLCELVEEGAEQRL"
     gene            3469..>3695
                     /locus_tag="AC16JC_00845"
     CDS             3469..>3695
                     /locus_tag="AC16JC_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163271.1"
                     /translation="MTRVLRGAIGTLGAAGVLALIRLGSFTPYTAERDVGAIVRLAWR
                     ARGERVNECRRRTPDELAKLPAHMRQEEVCEG"
BASE COUNT          547 a         1165 c         1376 g          607 t
ORIGIN      
        1 gatcgccgcg ccgtgggcga gcgtcaggaa ggcgataatc gcggccgtga gcgcgccgaa
       61 ggtgacgagc ggcaccgagc cctggtaggt gcgctcgcgt ttggccacga cgagcgtgcc
      121 gaagaagatg aacacgacga ccagggcccc caccgcgaac agaatgacgc cgaggtagaa
      181 ccaggaggtg gcctgcagcg ggacgtatga cgtgaacaag acgtcggcat ggcccgtcca
      241 catggtcgcc tcggtcatca cggcgccgat caccatcagg acgaaagcgg cccagccgag
      301 gcgtggcgtg gcgacgcggc tgttcagcac caccgcgctg gcgaagtaca gaatcgccat
      361 ttcgaagaag atgatgaaga aaatcagcat gctcatcccg tggatcgtca ggatgcggta
      421 gaaccagtcc gcggggagca ggtgcaccgc ctgccagcgg gtcagcagca cgaggatcgc
      481 ggcgatgccg ccgatcagca gcgcgaccgt ggcggccacg gcgttggccc ggatgagcga
      541 gtcggcggcc gcgtggatct tcaggttggt ccgcgggcag gtgcggaagt ccgtgggcgc
      601 ggtggcggcg gtcatggggc acctcctggc gccgcgaccg gcgccgcagc ggagtcgacg
      661 atgatgacct taccgaccat cgtgtggtgg ccgatgccgc agaactcgtc acagacgatg
      721 cggaagtccc cggcggcgtt ggggaccacc ttgaggccgt aatcgtagcc cgggacgacc
      781 tggaaattga tgttgacggg attgaggttg aagccatggt tcacgtcgag cgccgagagg
      841 tgcagcgtgt actcgacgcc cttctggagc ttcaggatcg gcgtccactt ccacatctgc
      901 gcgaggagat acacatcgct gccgggcggc ggggcgacga ccggaatgcc cgcctcttcg
      961 ccgacctggt actgtttgat gaactcctgc acccgctggt agtagcgcgt cggctcgacc
     1021 ttgtggcgga tgccggacgg gttctgctgg cccttcaggt gccagagcgg catcatggcg
     1081 aacagcacga ggcaccaggc gaacgcgacc gtcacccaga tgcgctcggc cccgtgcagc
     1141 gggagccacc agacgcgcgg cggagagtct aagccggtgt gcatgagtgg gtgtccttgg
     1201 atcagggaag gggggccggc ttgagcgaca ggatctcata gagaccccac gccgtgtaga
     1261 acaggaccat gatcacgatg cccgccgcga gcagcaggaa cggtcggtcg agcaggcgtt
     1321 ggatcagcgg gatgtcgccg tcgtcgcggc gggtctctgg ttgctctgcc atgatcggct
     1381 cctttccagg aaaaggccgt tcggcgtcgg cgctcaggcc ggcacggctg ctccctgcaa
     1441 cagctccgcg atcgtcgtct cgcgaaagaa ctccgcgacg cgatcggcga ccagcttcca
     1501 tttggtgtgt gcggcgcatg cggtccggtc gctgcactgc gcgcgtccca gcaggcagtg
     1561 gcgctcctcc cccaggctgc cgaacggcgc cactacctgc accacataca gccgatccgc
     1621 ggggaccgcc agtctgaatc cccccgactt gccgcgggtc gacaggagca cgccctcgcg
     1681 cacgagcgca tgcacgatct tggacaggta gtttctcggg atgcgcaggg ccgtagccat
     1741 ctcggtcacc tggacgagac gatccgtgcc atgctcggcg acgtacagca cggcgcgcaa
     1801 cgcgtactcg gcggtctggg agagcataat cttgtcttca tcatgataat atgatatttt
     1861 cacgccagca agcctaatca agggatccat ggccccggta gctgcgctga cgaagatcgt
     1921 gcgccgcggg ttcgcgcgcg cggacacggt cgcgaatcgc ctgtacggat ggcgctacaa
     1981 cccgctgtat cacagcgggg cgctcgtgct gatgctgttc gtggtgctgc tcgtcaccgg
     2041 cgtctacctg ctcttcgtct atcacatcag cgagccgtac gaatcggtcg cccgcattac
     2101 ccagcaggca tggctgggac gctggatgcg gggcgtgcac cggtacgcgt cggatgccgc
     2161 ggtcgtggcg gcgctgtttc acgcgttgcg catgctggtg caggggagaa gctgggggcc
     2221 gcgcaccctc gcctggatct cggggctcgt gctgctggcc tcggtgttcg tgtgcggctg
     2281 gacggggttc gtgatggtgt gggatgtcca gggtcaggtg atggcgcagg ccggggcgcg
     2341 catcttcgac ctgctgccga tcttcgggga gccgatcagc cgggcattca ccggcgagcg
     2401 ggccctcccg ggcgccttct tcttcttgaa cctgttcgcc cacgtggcgc tgccgatcgc
     2461 gctcttcatt ctcgtgtggg tgcacgtgtc acggctggcg cgcccgggtc tgttcccgcc
     2521 gcgctggctg acctgggcgg tcgtgggggc gctgttggca ctcgccgtcc tgtggccggt
     2581 ggcgatgacg ccgccggccg acgcgttcat gctgccgggc cgggcggcct acgactggtt
     2641 ctatgcgttc tggctgccgc tggcccgggc ggtgtcgccg gtcgccgtgt gggggatcgg
     2701 tcttgcgatc gtgacggccg gcctcgccat cccctggctg acgcgtcccc ggctgtcgcg
     2761 gcgtccgccg ccgtcggtgg tggatgaaaa gctgtgcacc ggttgcgagc agtgctacct
     2821 cgactgcccc tacgaggcga tcgcgatggt ggaccgcgcc gacgccgtgg ccaccggcaa
     2881 gtccgcgacg gtggcacggg tggaccccac gctgtgcgtg agttgcgggc tgtgcgcggg
     2941 gtcgtgcgcg ccgatggggg tgggtccgcc ggggcgtacc ggccgggcgc agctcacggt
     3001 ggtgcgcgac ttcatcgcgg cgcaccggcc cggagggggc gacgtcgtcg tggtggcgtg
     3061 tgagcgcggg gccggtgggg cgaccgccgc ggcggcgttc gacggcgcgc tggtctaccc
     3121 ggtgagctgc ggcggcaacc tgcacacgtc cgtggtggaa tacctgctgc gcgcgggcgc
     3181 gggcggcgtg ctgatcgtct cgtgtccgcc gcgcgactgc tggaaccgtg aggggccgaa
     3241 gtggctcgag gcccggctgt tccacgatcg ggaggcggag ctgcgcgagc gcgtggaccg
     3301 ccgccgcgtg cggctggtcc acgccgcggc gggcgagcgc gccgtggtgc gcgcggcgct
     3361 ggcggggttc cgggccgacc tcaccgccct cgaggcggcg accgtcgaat cggacatcga
     3421 tctcgaccgg ttgtgcgagc tggtggaaga aggcgcggag cagcggctat gacacgcgtg
     3481 ttgcgaggcg cgatcggcac gctgggcgcg gccggcgtcc tggcgctcat ccgcctgggg
     3541 tccttcacac cgtacacggc ggagcgcgac gtgggggcga tcgtgcggct cgcctggcgg
     3601 gcgaggggcg agcgggtgaa cgagtgccgc cggcgcacgc ccgatgagct ggccaagctg
     3661 ccggcgcaca tgcgccagga agaggtgtgc gaagg
//
LOCUS       DBNGZW010000035         3024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1094333, whole genome shotgun sequence.
ACCESSION   DBNGZW010000035 DBNGZW010000000
VERSION     DBNGZW010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3024
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1094333"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..635
                     /locus_tag="AC16JC_00850"
     CDS             <1..635
                     /locus_tag="AC16JC_00850"
                     /inference="COORDINATES: protein motif:HMM:NF014804.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5163272.1"
                     /translation="IQVEHPVTELVYGVDLVREQLRIAAGQPMRVAAGLLQPRGHAIE
                     CRITAEDPFAGFLPATGTVQYLRVPGGPGIRWDSGVESGNEVTLYYDPLLAKLIAWGE
                     NRAAALQRMERALRELVIVGIPTSQVFHLRVLADPEFRRGEIDITYLERAGARLLAEP
                     VSDPLVRPVAIVAALLAEAQRSGGAGGGPAVRGPSPQPAWLTEARREGLQ"
     gene            632..1921
                     /locus_tag="AC16JC_00855"
     CDS             632..1921
                     /locus_tag="AC16JC_00855"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HIR5163273.1"
                     /translation="MSTEVVAVSGIAAGGDGVGRLGDGRAVFLPRTAPGERVTLRAGS
                     LRMHRTFARGEAAEIVEPAAARVSPACPHYGADHCGGCQLQHLSYEAQLQAKRTIVGD
                     ALRRIGKLDIPDPEIRGAGEQWRYRTKISLAVRRRRGGHGEQEAPLVAGFHPYDRPDR
                     VFPLSDCRIADARLMSLWRQLQSHLGLLPEGATRLELRLDRDGTRHLVVESAGEPWAG
                     RTAERLRALIHEAGAGEVVCWWHPVDGAPRVIAGPATGFPATAFDQVNPLMDRAARRW
                     AVEGLGDVRGKVAWDLYGGTGDTALLLAEGGARVVSVDADEKAVAWARGRSDLAAFGD
                     GVRCIAGRVEDVFPGLPEPDVVVVNPPRSGLHWDVALRLTGDPVSRLAYLSRDPATLA
                     RDLQRLSVNYQVRAVQAFDLFPQTAQVETVVQLEAAR"
     gene            1918..2442
                     /locus_tag="AC16JC_00860"
     CDS             1918..2442
                     /locus_tag="AC16JC_00860"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HIR5163274.1"
                     /translation="MKYYIRIGARTVELEVDGGRVNVDGVWLEAHLAAIPGTPLYHLL
                     LAGESWTVAAQPLAGRGEGGGARWALGAVGERAEVEAVDERTRAIQALTGRKVGGPTG
                     GVVRAPMPGLVVRVEVLEGQQVGAGAPLVVVEAMKMENELRAQRAGRVVKVHVRAGTA
                     VEKGTPLVTLEPSS"
     gene            2505..2948
                     /gene="rplM"
                     /locus_tag="AC16JC_00865"
     CDS             2505..2948
                     /gene="rplM"
                     /locus_tag="AC16JC_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013638844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR5163275.1"
                     /translation="MKTFTLRKEDVTHQWFVVDAAGQPLGRLASRVAQILRGKHKPTF
                     TPHLNSGDAVIVINAERVKLTGRKLEQKRYFRHTGYMGHERFKPVKNVLATHPERVIE
                     QAVWGMLPKTTLSRQHVKQMLKVYAGPTHPHAAQQPQPLLLEGTK"
BASE COUNT          450 a          947 c         1193 g          434 t
ORIGIN      
        1 gcatccaggt cgagcatccc gtgacggagc tcgtctatgg cgtcgacctg gtgcgcgagc
       61 agctgcgcat cgccgcgggt cagccgatgc gggtggccgc cggcctgttg cagccccgcg
      121 gccacgcgat cgaatgtcgg atcacggccg aggatccgtt cgccgggttc ctgccggcga
      181 cgggcacggt gcagtacctg cgggtaccgg gcgggccggg cattcgctgg gacagcggcg
      241 tggaatcggg caacgaagtc acgctctact acgatcccct gctggccaag ctcatcgcct
      301 ggggcgagaa tcgcgcggcc gcactgcagc gcatggagcg tgcgctacgc gagctggtga
      361 tcgtggggat ccccacgtcg caggtgtttc acctgcgcgt gctggccgat cccgagttcc
      421 ggcgcggcga gatcgatatc acctacctcg agcgcgccgg cgcgcggctg ctcgccgagc
      481 ccgtgagcga tccgctggtg cggccggtcg ccatcgtcgc ggccctgctc gccgaggcgc
      541 agcggagcgg cggtgccggg ggaggaccag ccgtccgagg accgtctccc cagccggcct
      601 ggttgaccga ggcgcgccgg gaagggctgc agtgagcacc gaagtggtgg cggtctcggg
      661 gatcgctgcg ggcggggacg gagtggggcg gctgggtgac ggccgggcgg tgttcctgcc
      721 gcgcaccgct ccaggagaac gagtgacgtt gcgggcgggc agcctgcgca tgcaccgcac
      781 gttcgcgcgc ggcgaggccg ccgagatcgt cgaacctgcg gcggcccggg tctcgccggc
      841 ctgcccgcat tacggcgccg accattgcgg cggctgccag ctgcaacacc tgtcctacga
      901 ggcgcagctc caggcgaagc ggacgatcgt cggggacgcg ttgcggcgta tcggcaagct
      961 cgacattccg gatcccgaga tcaggggggc gggcgagcag tggcgctacc ggaccaagat
     1021 cagcctcgcc gtgcggcgca ggcgaggagg gcacggggag caggaggcgc cgctcgtcgc
     1081 cggcttccat ccgtacgacc ggccggaccg tgtgttcccg ctgtccgact gccgcatcgc
     1141 cgacgcgcgg ttgatgtccc tgtggcggca gttgcagtcc catctcggat tgctccccga
     1201 gggcgccacg cgcctggaac tgcggctcga ccgggacgga acgcggcacc tcgtggtgga
     1261 gtccgcgggt gagccatggg caggccgcac ggccgagcgg ctgcgcgcgc tgatccacga
     1321 agcaggggcc ggcgaggtcg tgtgctggtg gcacccggtg gatggggcac cgcgcgtgat
     1381 cgccgggccg gccacgggat ttcccgcgac ggcgttcgat caggtgaacc cgctgatgga
     1441 ccgtgccgcc cgccgctggg cggtggaggg gctcggcgac gtgcgtggca aggtggcgtg
     1501 ggacctctac ggcggcaccg gggacacggc gctgttgctg gccgagggtg gagcgcgggt
     1561 ggtcagtgtg gatgccgacg agaaggccgt ggcctgggcg cgtggcaggt ccgatctcgc
     1621 cgcgttcggc gatggagtgc gctgcatcgc cggcagagtc gaggacgtct ttcccgggct
     1681 tcccgagccg gacgtggtgg tggtaaaccc gccgcgatcc ggtctgcatt gggacgtcgc
     1741 cctgcggctg acgggcgatc ccgtgtcgcg cctcgcctac ctgtcgcgcg atccggcgac
     1801 gctcgcgcgc gatctgcaac ggctcagcgt gaactaccag gtgcgtgccg tgcaggcgtt
     1861 cgatctgttt ccgcagacgg cccaggtgga gacggtggtg cagctggagg ccgcgcggtg
     1921 aagtactaca tcaggatcgg agcccgcacg gtggagctgg aggtggacgg cggccgggtg
     1981 aacgtcgatg gcgtgtggct cgaagcccac ctggcggcca ttcccggcac gccgctctat
     2041 cacttgctgc tggccgggga gtcgtggacc gtcgccgcgc agcccctggc gggacggggt
     2101 gagggcggtg gggcgcgctg ggcattgggg gcggtggggg agcgggccga ggtggaggcc
     2161 gtggatgaac ggacgcgggc gatccaggcc ctcacgggcc gcaaggtggg cgggccgacg
     2221 gggggcgtcg ttcgggcgcc gatgccggga ttggtggtgc gcgtggaagt gctggagggc
     2281 cagcaggtcg gcgccggagc cccgctggtc gtggtggagg cgatgaagat ggaaaacgag
     2341 ctgcgggcgc agcgggccgg gcgcgtcgtc aaagtgcatg tccgggcggg aacggcggtg
     2401 gagaagggga cgccgctcgt gacgttggaa ccctcaagtt gacagggcct tagcgccccg
     2461 ggtatactgc cagtctgtca aaaatcggga cgacactggc agccatgaag acgttcacgt
     2521 tgcgcaaaga agacgtcacg caccaatggt tcgtggtgga cgccgccggg caaccgctcg
     2581 gccggctggc gagccgtgtc gcgcagatcc tgcgcggaaa acacaagccg acgttcactc
     2641 cgcacctgaa cagcggtgac gcagtcatcg tcatcaacgc cgagcgggtg aagctcacgg
     2701 gccggaagct ggaacagaag cgctacttcc ggcacaccgg gtacatgggt cacgagcggt
     2761 tcaagccggt caagaacgtg ctggccacgc atccggagcg ggtgatcgag caagcggtgt
     2821 gggggatgct tcccaagacc acgctgtcgc gccagcacgt gaagcagatg ctcaaggtgt
     2881 acgcgggacc gacgcaccca cacgcggcgc agcagccgca gccgctcctg ctcgagggga
     2941 cgaaatgact gagacgccgg agctgcgctg gcacgccgtc gggcgccgca aggagagtgt
     3001 ggcgcgggtg tacctgacgc cggg
//
LOCUS       DBNGZW010000036         4517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1435291, whole genome shotgun sequence.
ACCESSION   DBNGZW010000036 DBNGZW010000000
VERSION     DBNGZW010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4517
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1435291"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /gene="coaBC"
                     /locus_tag="AC16JC_00870"
     CDS             <1..954
                     /gene="coaBC"
                     /locus_tag="AC16JC_00870"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR5163276.1"
                     /translation="PDLVVVAPATAQLLARAAMGMADDLLTAILLARPGAVLAAPAMN
                     DAMFAHPGTRANLRALAARGWTFIGPETGALAEGPSERQGRMSEPETILAAVLRQLGA
                     DTRWAGRKVVVSAGPTREHLDPVRVLTNPSSGRMGWALAEAARDRGARVVLVAGPTTL
                     GPPPGVETVRVETTDAMQRAVQHALTGASALFMAAAPADYRPAKTDQRKRPRGRGRIT
                     LALEPTPDILASLKRPSGCLVVGFALETGDGLARAREKLKSKRLDYVVLNDALEPGAG
                     FEVTTNKVTLLGRSGAPLELPLLDKRDVAQRILDAVEHELT"
     gene            951..1772
                     /locus_tag="AC16JC_00875"
     CDS             951..1772
                     /locus_tag="AC16JC_00875"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR00758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR5163277.1"
                     /translation="MSDGRALARAYLEQQRLLGTDAVTLPPSAAGTAPEPSPDPGSTP
                     GVRPGAGDHPGRTPANAPAPAKAELPPAGISYDPPARDLFAADPLQQVTQLAQVAQLV
                     AECTKCRLCESRTHTVPGEGPDAARLVCVGEGPGRTEDETGRPFVGQAGELLTKILAA
                     IGLSRERVYICNVVKCRPPQNRTPQYDEIASCVPYLFRQLELLAPKVILAMGNTAAQT
                     LLNTKQSLGALRTKVHRFRGIPLIVTYHPAALLRNPNWKKPTWDDVRIAHRLLDD"
     gene            1735..3144
                     /gene="dnaB"
                     /locus_tag="AC16JC_00880"
     CDS             1735..3144
                     /gene="dnaB"
                     /locus_tag="AC16JC_00880"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013279247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HIR5163278.1"
                     /translation="MTSASPTVSSTTDLDVAGRRTPWSNDAEQAVLGAMLLDQDAALK
                     AAEFLDDTMFYKEAHRVLFRAMVSLTERGDVIDPVTLRDELGKRGDLERAGGMEYIAT
                     LIDVVPTAANVEYHTRIVRDKAVLRRLVEAATEIIQDVYTTRGDSSEVLDNAEQRIFQ
                     VAQSRRSEEFLRLKELIWPTMERIEQMQGRAGALTGVPSGFTDLDEMTAGFQRSDLVI
                     LAARPSMGKTAFALNVVQHAAIEHGVRVAIFSLEMSKDQLVQRLLCSEGLVDAQRLRR
                     GTLRDEDYPKLARAAGLLSSAQIWIDDSAGLTPLAMRSKARRLKAEHDIGLIVIDYLQ
                     LMQGPQNSENRQQEISYISRSLKALAKELDIPVLALSQLSRAPEQRGGEHRRPQLSDL
                     RESGAIEQDADVVCFIYRPEMYEPTDKDGNSVEGLAELHVGKQRNGPTGTVQLYFKKE
                     YTRFDNRTNRDAPPGVSVG"
     gene            3137..4495
                     /gene="radA"
                     /locus_tag="AC16JC_00885"
     CDS             3137..4495
                     /gene="radA"
                     /locus_tag="AC16JC_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009726114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR5163279.1"
                     /translation="MARASSLFRCTDCGAEQPKWAGRCDACGAWNTLVEEPLADRRSG
                     GRAVAGARAFDRPPVRLSALSRQELVRWRTGLGELDFVLGGGIVPSSVILVGGEPGIG
                     KSTLLLQAAAHLERAGVRTLYVSGEESPEQLRLRADRLDADAGAVHVLGETNLEAILH
                     HAGAIGAQAVFLDSIQTTYTEALEGAPGNVGQVRECAARLMRFAKEAGPAVFLVGHVT
                     KGGGIAGPRTLEHIVDTVLYFEGEATLDHRVLRAVKNRFGSVDEIGVFRMTATGLEPV
                     ADPAAAFLAGRHAGVSGSAVTALMEGTRPLLVEVQALASKPGFGTPQRVATGLDQRRL
                     AVLLAVLERRGGLPVGALDVFVNVTGGVRLLEPSTDLAVLAALTSSVHDRPVPADALF
                     LGEVGLGGEVRPVAGLERRLTEAQRQGFRRVFLSSRSRVAGGLEVVAIAHVGELARRL
                     AA"
BASE COUNT          712 a         1518 c         1607 g          680 t
ORIGIN      
        1 ccggatctgg tggtcgtggc ccccgcgacg gcgcagctgc tggcccgcgc ggcgatgggc
       61 atggccgacg atctgctgac cgcgattctg ctcgcgcgtc ccggcgccgt gctcgcggcc
      121 cccgccatga acgacgccat gttcgcccac ccgggcaccc gggccaatct gcgggcgctc
      181 gccgcgcggg gctggacctt catcgggccc gagacgggcg cgctcgccga aggcccgagc
      241 gagcggcagg gccggatgag cgagccggag acgatcctcg cggcggtgct gcgtcagctc
      301 ggggcggata cccgctgggc cgggcgcaag gtggtggtga gcgcgggccc cacgcgcgag
      361 catctcgatc cggtccgcgt gctcaccaat ccctcgagcg gacgcatggg gtgggcgctg
      421 gccgaggcgg cgcgcgaccg cggcgcgcga gtcgtgctcg tggcggggcc aacgacgctc
      481 ggcccgcccc cgggcgtgga aacggtgcgc gtcgaaacca cggacgcgat gcagcgcgcg
      541 gtgcagcacg cgctcaccgg cgcgtccgcg ctgttcatgg ccgcggcccc ggccgactac
      601 cgtcccgcga agacggacca gcgcaaacgc cctcgcggcc gtggacgaat cacgttagcg
      661 ttggagccga cgccggacat tctcgcgagc ctgaagcggc cgtccggttg cctggtggtc
      721 ggattcgcgc tcgagaccgg tgacgggctc gcgcgcgccc gggagaagct gaaatcgaag
      781 cggctggact acgtggttct gaacgacgcg ctggagcccg gtgcggggtt cgaggtgacc
      841 acgaataaag tcacgctgct cgggcggtcg ggagccccgc tggagctccc cctgctcgac
      901 aagcgcgacg tggcgcagcg aatcctcgac gcggtggagc acgagttgac gtgagcgacg
      961 ggcgggcact ggcgcgcgcc tatctggaac agcaacggct cctcggaacg gatgcggtga
     1021 cgctgccgcc gtccgcagcg ggaactgctc ccgaaccgtc tccggatccc ggttccactc
     1081 cgggcgtgcg accgggagcg ggtgaccatc cagggagaac accggcgaac gccccggccc
     1141 cggcgaaggc ggagctgcca cccgccggca tcagctacga tccgccggca cgggacctgt
     1201 tcgcggccga cccgctgcag caggtcacgc agctggcgca ggtggcgcag ctggtcgcgg
     1261 agtgcacgaa atgccgtctg tgcgagagcc gcacccacac cgtcccgggt gagggcccgg
     1321 acgccgcccg gctcgtctgc gtgggcgagg ggccgggccg gaccgaggac gagaccgggc
     1381 ggcccttcgt ggggcaggcc ggcgagctcc tcaccaagat cctcgccgcg attggcctgt
     1441 cgcgcgagcg ggtgtacatc tgtaacgtgg tgaagtgccg gccgccgcag aatcgcacgc
     1501 cgcagtacga tgaaatcgcg tcctgcgtgc cctatctgtt ccggcagctc gagctgctgg
     1561 cgcccaaggt gatcctggcc atggggaaca cggcggcgca gacgctgctc aacaccaagc
     1621 agtcgctcgg ggcgctgcgc accaaggtgc accggttccg gggtattccg ctgatcgtga
     1681 cctaccaccc ggccgcattg ctccgcaacc cgaattggaa gaagccgacc tgggatgacg
     1741 tccgcatcgc ccaccgtctc ctcgacgact gacctcgacg tcgccggccg gcgcacgccc
     1801 tggagcaacg atgccgagca ggccgtgctc ggcgcgatgc tgctggacca ggacgcggcg
     1861 ctcaaggcgg ccgagttcct cgacgatacg atgttctaca aggaggcgca ccgcgtcctg
     1921 ttccgggcga tggtgagcct caccgagcgc ggcgacgtga tcgatccggt cacgctgcgc
     1981 gacgagctgg gcaagcgggg cgatctcgag cgggccggcg gcatggagta catcgccacc
     2041 ctgatcgacg tcgtaccgac cgcggccaac gtcgagtatc acacgcgcat cgtccgcgac
     2101 aaagcggtac tgcgccggct ggtcgaggcg gcgaccgaga tcatccagga cgtctacacg
     2161 acacggggcg attcgagcga agtcctcgac aacgccgaac aacggatctt ccaagtagct
     2221 cagtcccgtc gctccgagga gttcctgcga ttgaaggaac tcatctggcc gaccatggag
     2281 cgaatcgagc aaatgcaggg acgagctggc gctctcacgg gagtgcccag cggcttcacc
     2341 gatctcgacg agatgactgc agggtttcag cgatccgacc tggtgatact cgcggcgcgc
     2401 ccttcgatgg gtaagacagc gtttgcgctc aacgtagtcc agcacgcggc aatcgaacac
     2461 ggcgttcgag tggcgatctt ttccctggaa atgtctaagg atcagcttgt tcaacgtctg
     2521 ctctgttcag aagggttggt cgatgcacaa cgactccgtc ggggcaccct gcgcgacgag
     2581 gattacccaa agctcgcccg ggccgccggc ctcctgagct cagctcaaat ctggatagac
     2641 gactccgctg ggcttacccc actcgcgatg cgttcgaaag cacggcgcct caaggccgag
     2701 cacgacattg gtctgatcgt catcgactat ctgcaactga tgcaaggccc acagaacagc
     2761 gagaacaggc agcaggaaat ctcctacatc tcgcgttccc ttaaggcgct cgcaaaagag
     2821 ctcgacatac ccgtgttggc actctcacag ctatcgcggg ctccagagca gcgaggaggt
     2881 gagcaccgca ggccacagct ctcggacctg cgggagtcgg gagcaatcga gcaagatgcc
     2941 gatgtggtgt gtttcattta ccgtccggag atgtacgagc cgaccgacaa ggatggcaac
     3001 agcgttgagg gtctggctga gctgcacgtg gggaagcaac gcaatggtcc taccggcact
     3061 gtccaactct acttcaagaa ggaatacacg cgcttcgaca accgcacgaa ccgcgatgcg
     3121 ccgcccggag tgagcgttgg ctagagcctc ctcgctgttc cgctgcaccg actgcggcgc
     3181 cgagcagccc aagtgggcgg ggcgctgcga tgcctgcggg gcgtggaata cgctggtgga
     3241 agagccgctg gcggacaggc ggtcaggcgg tcgggcggtc gctggggcgc gggcctttga
     3301 ccgaccgccc gtccgcctgt ccgccctgtc taggcaggag ctcgtccgct ggcggacggg
     3361 gttgggcgag ctcgatttcg ttctcggggg cgggatcgtc ccctcatcgg tgatcctggt
     3421 ggggggcgag ccggggatcg gcaagtccac gctgctgctg caggcggccg cccacctcga
     3481 gcgcgcgggg gtgcgcacgc tgtacgtgtc gggcgaggag tcgcccgagc agctgcggct
     3541 gcgcgccgac cggctcgatg ccgacgccgg cgccgttcac gtgctgggcg agaccaacct
     3601 cgaggcgatt ctgcaccatg ccggcgcgat cggcgcccag gcggtcttcc tcgattcgat
     3661 ccagacgacc tacacggagg cgctggaagg cgccccgggg aacgtgggcc aggttcgcga
     3721 gtgcgccgct cgcctgatgc gtttcgcgaa ggaggccgga ccggcggtgt ttctcgtggg
     3781 acacgtgacc aagggcggcg ggatcgcggg tccgcgcact ctcgagcaca tcgtggacac
     3841 cgtactctac ttcgagggtg aagcgacgct cgaccaccgg gtgctccggg cggtgaagaa
     3901 ccggttcggg tcggtggacg agatcggcgt gttccgcatg accgcgaccg ggctcgagcc
     3961 ggtggccgat cccgcggccg cgtttctcgc cgggcgccac gcgggagtct ccggttcggc
     4021 cgtcacggca ctgatggaag gcacgcgccc cctgctggtg gaggtgcagg cgctcgcctc
     4081 caagccgggc ttcgggacgc cgcagcgcgt ggccacggga ctcgatcagc ggcggctcgc
     4141 cgtactcctc gcggtgctgg agcgccgcgg gggcctgccg gtcggcgccc tcgacgtgtt
     4201 cgtcaacgtt acgggcggtg tccgcctgct cgagccatcc accgatctcg cggtgctcgc
     4261 cgcgctgacc tccagcgtcc acgaccggcc cgtgccggcc gacgcgctgt tcctgggcga
     4321 agtgggcctg ggcggggaag tgcgcccggt cgccgggctc gagcgccggc tgaccgaggc
     4381 ccagcgccag gggttccgcc gcgtcttcct gtcgtcgcgc tcccgcgtgg ccggcggact
     4441 cgaggtcgtg gcgatcgcgc atgtcggtga gctcgcccgc cggctcgccg cctgacgtcg
     4501 ccgtcgtgat tgtggcg
//
LOCUS       DBNGZW010000037         4725 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1435320, whole genome shotgun sequence.
ACCESSION   DBNGZW010000037 DBNGZW010000000
VERSION     DBNGZW010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4725)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4725)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4725
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1435320"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..308
                     /locus_tag="AC16JC_00890"
     CDS             <1..308
                     /locus_tag="AC16JC_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163280.1"
                     /translation="GTSLHTFSSEAPPARPARPGIPNTTALWRPEPRAFPKAAGLHRV
                     TWQPNTGRPAGFGGFGRRGPEEPTVTLPGEFTARLTVNGKSYTQTFSVRPDPRSLAE"
     gene            336..704
                     /locus_tag="AC16JC_00895"
     CDS             336..704
                     /locus_tag="AC16JC_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR5163281.1"
                     /translation="MCRDLRALPVILLAASVVPHPAAAQSPALFGELHWRQIGPPRAG
                     RARALSGVPSQPNVFYVGFDNGGVWRTTDYGSNWVPLFDDQPTGSIGAIAVAPSNPDV
                     IYVGSGAGIIRPDLATGDGV"
     gene            711..3611
                     /locus_tag="AC16JC_00900"
     CDS             711..3611
                     /locus_tag="AC16JC_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR5163282.1"
                     /translation="MDAGRTWTHLGLRETQMIANVAVDPTNPDRVFVAALGHPYGPNA
                     ERGIFRSTDGGRTFQKVLYTDEYTSGNDVVIDPGTPGVVYAALWQQQQQFREGADFGG
                     AGGGIFKSTDGGTTWSPLTEGLPDVIEANLAVAPSDPRIVYAMVAGIAAGARPSTTGT
                     VGFYKSVDGGAHWQLAADTTARPADPRPLARIGGGDLPTLAVDPKNPNIVYSSTIVLW
                     RTEDAGRTWSAVRGAPGGDDYQKTWINPNNPDILLTVADQGAVISANRGASWSNWYTQ
                     PTAAMYHVSTDNAFPYRVCGGQQDAGSACVDSRSMDGAITFHDWHPANIQEYGIAAPD
                     PRDPDLVYGSARTNVSLYNRRTGQTTNVGPGADARGAQYGRNVRTMPLIWSPVNPSVL
                     FYTSNAVWKTVDRGHSWTRISPDLTRQTWEVPSSAGKYASEVTVAPLGAITALSPSHG
                     DVTVIWAGTDDGNIQVTMDGGVRWVNVTPPAIKPWTRIFNIEAGHFDTRTAYAAANTL
                     RVDDNNPHFWRTHDGGKSWTEINTGIAAGAVANSIREDPRTPGLLYASTDTQVWVSFD
                     DGAHWRSLRLDMPAISVRDIQVKDDSSCLCTDLVAGTHGRGFWILDDVTPLRQMAQAQ
                     AAGDAYLFKPQAAVRVRFGTNDPTPWPPELPAGENPPPGGIIDYYLAADVSSPVTLEI
                     LDAAGALVRSYSSGDSVITPDPARDPAGYDKVCQADPRATYCSLPLYWPAPQLRLSTR
                     AGMHRFTWDLHFDPVGDRGPREGGATGAVPRRTYPAVNAPWAPPGAYTVRLTVNGKRF
                     TQPLTLRLDPRVRTPALGLRRLAALSREMYDGARVAYGAYVQARALAARLDSAGGTDA
                     AAFKAQVESLAPAPRAAPREAAAGGFFRGPAGPPTLETLSGTLLSAAMAMQNADVTPT
                     ASQVAACDSARARSRDLMGRWSTLKGAGLTALNAKRKAAGLPEIGLPEAPRP"
     gene            complement(3608..>4725)
                     /locus_tag="AC16JC_00905"
     CDS             complement(3608..>4725)
                     /locus_tag="AC16JC_00905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5163283.1"
                     /translation="KEEDYIRRNIAGQNSAQAKGRRARLARLPRLSAPPGEREAMSVR
                     FADAERGGDQVLVADRLRVAIADRTLVRDFSAVARRGDVIALVGPNGAGKSTLLATLF
                     GARAAAAGEARLGAGVVAEWFRQDLAQVPEGRSIYDCIADVRGRWTRGHIQNHLGAFG
                     FSGDEVQRSTSIISGGERARVALALLALRPANLLVLDEPNNDLDVESIEALEDGLTEY
                     EGTVILVSHDRAFLRELATRVWAFDGDRIQDYAGTFVEWEQQQAERTGANRAARAEPG
                     ASPVTRTEVRKAAAARRESQEARRAAQREADVREQAVHAAEARIGELERALADPALYD
                     GRAEGAREAGRLSAALAQAKRALDEALARWAEASDAL"
BASE COUNT          696 a         1824 c         1571 g          634 t
ORIGIN      
        1 aggggacgag cctgcacacg ttctcgagcg aagccccgcc cgcgcggccg gcgcggcccg
       61 ggatccccaa cacgaccgca ttgtggcggc ccgagccccg ggccttcccg aaggcggccg
      121 gcctgcatcg cgtgacctgg cagccgaaca ccggccggcc tgccgggttc ggagggtttg
      181 ggcgccgtgg acccgaagag ccgaccgtca ccctgccggg cgaattcacc gccaggctca
      241 ccgtcaacgg caagagctac acgcagacct tctcggtgcg tcccgacccg aggagcctgg
      301 cggaatagca ccgcacccac cgagccgagg atctcatgtg tcgtgacctt cgcgccctcc
      361 ccgtcatcct gctcgcggcc tcggtcgtcc cccatccggc ggccgcccaa tccccggcgc
      421 tcttcggcga gctgcactgg cgccagatcg gaccgccgcg cgccggccgc gcccgggccc
      481 tctcgggcgt gccgagccag cccaacgtct tctatgtggg attcgacaac ggcggcgtgt
      541 ggcgcacgac cgactacggc tccaactggg tgccgctgtt cgacgaccag ccgacgggct
      601 cgatcggcgc catcgccgtg gccccctcca accccgacgt catctacgtg gggagcggcg
      661 ccgggatcat ccggcccgac ctcgccaccg gcgatggcgt ctaaaagtcc atggacgccg
      721 gccggacgtg gacccatctg gggctgcgcg agacccagat gatcgccaac gtcgccgtcg
      781 atcccacgaa ccccgaccgg gtgttcgtcg ccgcgctggg ccatccctac ggccccaatg
      841 ccgagcgggg gatcttccgg tccaccgacg gcggccggac gttccagaag gtcctctata
      901 cggacgagta cacgagcggc aacgacgtcg tcatcgaccc gggcaccccg ggcgtcgtct
      961 acgccgcgct gtggcagcaa cagcagcagt tccgggaagg cgccgacttc ggcggggcgg
     1021 gcggcggcat cttcaagtcc accgatggag gcacgacgtg gagcccgctg actgagggcc
     1081 tccccgacgt catcgaagcc aacctcgccg tcgcgcccag cgatcctcgc atcgtgtacg
     1141 ccatggtagc cggcatcgcc gccggcgccc gccccagcac gacggggacc gttggcttct
     1201 acaagtccgt ggacggcggc gcgcactggc agctcgccgc cgataccacc gcacggcccg
     1261 cggacccgcg cccgctggcc cgtatcggcg gtggggatct gcccacgctc gcggtggacc
     1321 cgaagaaccc gaacatcgtg tacagctcca cgatcgtcct gtggcgcacc gaggatgccg
     1381 gccgcacctg gtcggcggtg cgcggcgccc ccggcggcga cgactaccag aagacctgga
     1441 tcaacccgaa caaccccgac attctcctca cggtcgccga ccagggcgcg gtgatttcgg
     1501 ccaatcgcgg cgcgtcgtgg agcaactggt acacgcagcc caccgccgcg atgtaccacg
     1561 tctcgaccga caacgccttt ccctaccgcg tctgcggcgg gcagcaggac gccgggtccg
     1621 cctgcgtgga cagccgctcg atggacggcg cgatcacctt ccacgactgg catccggcca
     1681 acatccagga gtacggcatc gccgcaccgg accccaggga cccggacctg gtgtacggca
     1741 gcgcccgcac caacgtctcg ttgtacaacc gcaggaccgg acagacgacc aacgtcggtc
     1801 ccggggcgga cgcccgcggg gcgcagtatg gccggaacgt ccgcaccatg ccgctcatct
     1861 ggtcgcccgt gaacccgagc gtgctcttct atacctcgaa cgccgtgtgg aagacggtcg
     1921 accgcggtca cagctggacg cggatcagcc cggacctgac gcgccaaacc tgggaggttc
     1981 cctcgagcgc cgggaagtac gccagcgagg tcaccgtggc tccgctgggc gcgatcaccg
     2041 cgctctcgcc gtcgcacggc gacgtgacgg tgatctgggc cggcaccgac gacggcaaca
     2101 tccaggtgac gatggacggc ggcgtacggt gggtcaacgt caccccgccc gcgatcaagc
     2161 cctggacccg catcttcaac atcgaggcgg ggcacttcga cacgcgcacc gcctacgccg
     2221 cggccaacac gctgcgggtg gacgacaaca acccgcactt ctggcgcacc cacgacggtg
     2281 ggaagagctg gaccgagatc aacaccggga tcgcggccgg cgccgtggcc aactcgatcc
     2341 gcgaggatcc gagaacgccc gggctgctct atgcctcgac cgatacgcag gtctgggtgt
     2401 cgttcgacga cggcgcgcac tggcgctcgc tgcggctcga catgcccgcg atttcggtgc
     2461 gcgacatcca ggtgaaggac gacagcagct gcctctgcac cgacctcgtg gcgggcacgc
     2521 acgggcgcgg cttctggatc ctggacgatg tgacgccgtt gcggcagatg gcgcaggctc
     2581 aggcggcggg cgacgcctac ctcttcaagc ctcaggccgc ggtccgcgtg cgcttcggca
     2641 cgaacgaccc cactccgtgg ccgcccgagc tgccggcggg cgagaatccg ccgccgggcg
     2701 ggatcatcga ctactacctc gcggccgatg tctcaagccc ggttaccctc gagatcctgg
     2761 acgcggccgg cgcactcgtc cgctcgtatt cgagcgggga ttcggtgatc acgcccgatc
     2821 cggcgcgcga cccggccggc tacgacaagg tctgtcaggc ggacccgcgc gcgacgtact
     2881 gctccctgcc cttgtactgg cccgccccgc agctgcgact ctcgacccgg gccggaatgc
     2941 accggttcac ctgggatctg cacttcgacc ccgtgggcga tcggggaccg cgagagggag
     3001 gcgccaccgg cgcggtgccg cgccgcacct atccggcggt gaacgccccg tgggcgccgc
     3061 ccggcgccta caccgtgcgc ctcacggtga acggcaaacg cttcacccag ccgctcacgc
     3121 tgcggctcga tccacgggtg cggacgccgg cactcggcct gcggcggctc gccgcgctgt
     3181 cgcgcgagat gtacgacggc gcgcgggtcg cgtacggtgc gtacgtgcag gcccgcgccc
     3241 tcgcggcgcg actcgacagc gcgggtggca cggacgccgc cgccttcaag gcgcaggtcg
     3301 agtccctggc acccgcgccg cgagccgcgc cccgcgaggc ggcggccggc ggcttcttcc
     3361 gggggcccgc cgggccgccg acgctggaaa cgctcagcgg aacgttgctg tccgccgcga
     3421 tggcgatgca gaacgccgac gtcacgccga cggcgagtca ggtggcggct tgtgattccg
     3481 cgcgcgcccg gtcgcgcgat ctcatgggcc gctggagcac gctcaagggc gccggactca
     3541 ccgccctgaa cgcaaagcgc aaggctgccg gcctgccgga gatcgggctc cccgaagccc
     3601 cgaggcccta gagcgcgtcg cttgcctccg cccagcgggc cagagcctcg tcgagggcgc
     3661 gcttcgcctg ggccagggcc gcgctcaaac ggcccgcctc gcgtgcgccc tccgcccggc
     3721 cgtcgtacag cgccgggtcg gcgagcgcgc gctccagttc gccgatccgt gcctcggccg
     3781 catgcaccgc ctgctcgcgg acgtcggcct cccgttgcgc cgcgcgccgc gcctcctggc
     3841 tctcgcgccg cgccgcggcg gccttccgca cctcggtccg cgtgaccggg ctggccccgg
     3901 gctccgcgcg cgcggctcga ttcgcgccgg tccgctcggc ctgctgctgt tcccactcca
     3961 cgaaagtccc ggcgtaatcc tggatccggt cgccgtcgaa ggcccacacg cgggtcgcca
     4021 gctcccgcaa gaacgcgcgg tcgtggctca ccaggatgac cgtgccctcg tactccgtga
     4081 gaccgtcctc cagcgcctcg atcgactcca catccaggtc gttgttgggt tcgtcgagca
     4141 cgaggaggtt cgccggacgc aacgcgagca gcgccagcgc cacccgagcg cgctccccgc
     4201 cggaaatgat cgaggtgctg cgctgcacct cgtctcccga aaacccgaag gcgccgaggt
     4261 gattctggat gtggcctcgc gtccagcggc cccgcacatc cgcaatgcaa tcgtagatcg
     4321 accgtccctc gggcacctgg gccaggtcct gccggaacca ctccgccacg actccggcgc
     4381 ccagccgcgc ctcccccgcc gcggccgcgc gcgcgccgaa cagggtggcg agcagggtgg
     4441 acttgcctgc cccgttcgga cccaccagcg cgatcacatc gccgcgccgg gccacggccg
     4501 agaagtcccg caccagggtc cggtccgcga tcgccacgcg caggcgatcc gccaccagga
     4561 cctggtctcc gccccgctcg gcgtcggcaa agcgcacgct catcgcctcg cgctctcccg
     4621 gcggcgcaga gagccgcggc agccgggcga gccgagcacg gcgccccttc gcctgggcgc
     4681 tgttttgccc tgcgatattg cgccggatgt agtcctcctc cttgg
//
LOCUS       DBNGZW010000038         8295 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_685484, whole genome shotgun sequence.
ACCESSION   DBNGZW010000038 DBNGZW010000000
VERSION     DBNGZW010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8295)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8295)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8295
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_685484"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            141..1292
                     /locus_tag="AC16JC_00910"
     CDS             141..1292
                     /locus_tag="AC16JC_00910"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HIR5163284.1"
                     /translation="MVLVTAATGPLAAQRVSRMPVEAANLVVQVLVTGGDDEEVGAGI
                     VVASSNRIVIATAAHVVRAAQQGGSIRVVFQFARNDTVAAKIEQLDRELDLAVLSLAR
                     DGRSSIQFAFDRGGDPGALKIGTLVVPLGCPERRCWEPPVSGDRVISASARRVRFESF
                     FVSPGSSGGALFTRQWEVVGLVTEKNTQDGVAVGMSEVADRLRKWGYTVQLRPASIPR
                     AGYPTRLSLVGLAPTSGALTQSGRWPSGRLALLLRGQERIGWHLTAIRLAPQDLKVTG
                     GMVGTSVSLRAGRLGIAPFGEVGVARVNGRFNAGSYFVQTASGTVAVPVWQTVTNDVI
                     GVGVGADVELLVAPHVAVSGVVGHWSFRTDTPLPSIPDVFVGAGLKLAF"
     gene            1334..3430
                     /locus_tag="AC16JC_00915"
     CDS             1334..3430
                     /locus_tag="AC16JC_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005085 - guanyl-nucleotide exchange
                     factor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HIR5163285.1"
                     /translation="MRAIKALLAGALLTTCTNELAPTGAIVVRLEKGAAWPDTLAVAE
                     IASLAVSITGPDQQVVTGVTLAWGSTDSSVVTVTDAAAALHGIVSSRRTGEATIVVRV
                     TQTGFEPVELRAPVVVRPRGADSLLSVGDVDTIGLALQRVDPAFLTGATVAWGSSDPS
                     VVGVSALAADSTRAVITGRFSGTAQVTATIQSPLGRSTFQLPVAVFPLEISELPAWAP
                     TVNRTNTAAFAVQVKDALGRIRPGVKVQWHSTNETAFTVDSTGTVLGKTRGGGELVAS
                     VGVAPFQVVEHRAPLQVTEKWKAVSAGGDHSCAIAALDGTGYCWGSNAQGQLGLGFDA
                     SALGQTSLPRAVATSHKFTELEAGAAHSCGREGFQTLLCWGARDRGALGDGVCIPGGM
                     GSTCFPSAESPVTVVSGGIVSGVQVRLDRLIVGGTFSCIVTVNGGSGSFASRDLRCWG
                     TADDFGRGISFSGSGFADSAVILQPGLSGDADITDVSTGGAHLCTRTDDIWWVRCMGI
                     NDHAQLGDGTVGNDPLPSWLPKDFTIVGGDPVNPGGDGYPTSGLSAGGSHTCALDAVG
                     VLCWGSNASGQLGSAVAGDAVYPTRAALPVQAVVLSAGGDHTCALVTGGDVWCWGSNS
                     HGQLGRGTIGGSSSVPAPVSGGLTFVSISAGGAHTCGVTADGSLYCWGNNSSGQLGDG
                     TQTDWGTPVRVVESPQ"
     gene            3612..4223
                     /locus_tag="AC16JC_00920"
     CDS             3612..4223
                     /locus_tag="AC16JC_00920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR5163286.1"
                     /translation="MIFVPILVAGRSRERCAGPACRAGNRRSPRGPVHRCDPWNNVEV
                     QARSVHSARQATLARIGGAQCPESAVINRVESRVAMKCRRGFTVIEVTVAIVVLTVGL
                     LGLVTTAALVTRMLARGQRTAMAATFAQSRLERLRSAACIPAQRADGSDTLSRGSTWV
                     AINRWAFSGSSSPTTFRVRIVTISKTDRGAIRVDTSESSIPCR"
     gene            4256..5101
                     /locus_tag="AC16JC_00925"
     CDS             4256..5101
                     /locus_tag="AC16JC_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIR5163287.1"
                     /translation="MTLVEILVALMMLGVVSGAVYRVLLNNQRIYQAQTQHIELEQNI
                     RAAVAVLPAVLREIDATDGDIVAMSSTALTIRSMRWLGFICMPPALGGAVVGNTMTLR
                     AQPFYGSRGLTIPTDSMFIYYDGNQATRNDDDWALARPTDVANGLCPDGQPGLVVTMS
                     WTLALPNLAGGIPPGSPVRGFETVTYQLYQPPGDSLWYVGLQPAGETIQPIIGPVLPG
                     GGLRFAYFDSTGTVTTIPGRVARIDVTVRGRTSQAVQNAAGAGKSVAVDSVVTSVALR
                     NNRRY"
     gene            5137..6291
                     /locus_tag="AC16JC_00930"
     CDS             5137..6291
                     /locus_tag="AC16JC_00930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HIR5163288.1"
                     /translation="MKRILRDERGMALAIAIFALIVVGALVAGAFFAGTQEQRVGVNS
                     RRVQQSLGVAEAGIAERLATWRPDSLNQRGIYPADSAPIVLTPAPFGTGSYGGVVYKL
                     TPNLFLLDVSGSDWASRAGRTQGGSGARQRVGLLTRLAPVQFDIQASLTTQGTVNLKG
                     TATVDGNDQNPTGWLDCGPLAEAKAGIRVEPGASIDSGGSASVSGDPNVFRDPTLTDS
                     TFTRFSGITYAELAAEATVKLPGGNYKAQPAILDGKCDTRDQLNWGDGQNPASACGSY
                     FPTVHIAGSATLNGVQGQGVLLVDGDLTVDGGFEWFGIAIVQGQFQTGGGGKTPAHFW
                     GAILARNADLSVQDMAGHATVNYSSCSIRKALQGTSKPSMLRSRGWIQLY"
     gene            6450..6908
                     /locus_tag="AC16JC_00935"
     CDS             6450..6908
                     /locus_tag="AC16JC_00935"
                     /inference="COORDINATES: protein motif:HMM:NF025734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HIR5163289.1"
                     /translation="MSRRLQQAAVVFVVVVAAAQFIRPKRANPPIDASRTIQASVGTA
                     SGLSAVLDRACSECHSNATVWPWYSNIAPASWLMAYGVKRGRAAVNYSEWAGYPPERQ
                     RELLVASCQDASTGKMPGSLYTLLYSETRLSPQDVETICAASREVAREKP"
     gene            6905..7387
                     /locus_tag="AC16JC_00940"
     CDS             6905..7387
                     /locus_tag="AC16JC_00940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR5163290.1"
                     /translation="MTTPTLETKVHDFLAQKRIAVAGVSRDKSHHPAGNLIYERLKRT
                     GHDVFAVNPNMPSFEGDRCYPDLQSIPGGVDGVVIITRPETTQQIVRDCGTAGVRRVW
                     MHQSMAKGSSVSPDAVEYCRQHDISVIAGACPMMFGPGVDFGHTCMRWIMKLTGGLPT
                     "
     gene            7384..7869
                     /locus_tag="AC16JC_00945"
     CDS             7384..7869
                     /locus_tag="AC16JC_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163291.1"
                     /translation="MTRRQVAWMLILIVDVGYIAWGAMAAASPDHLLRGPGGKPILAA
                     AYEGYSHASWSQLVGTSPLTAGYMLVLYRMYGIYCAVFGLLASAIAVTAFRRGEAWAW
                     WALLVGNTIALVSAMRMDWIVNAIGPFELTEYLGLALVWGALAVTAPFRAAGQPVRAG
                     G"
     gene            7909..8175
                     /locus_tag="AC16JC_00950"
     CDS             7909..8175
                     /locus_tag="AC16JC_00950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163292.1"
                     /translation="MKLEVISRHPRESRHPTPYDIQDAGLARGIDADEHVVLIPDVRV
                     DGMGQIARFGWPFQLSSTAAGSPIVTSRSREPPGRESWDVVERP"
     gene            complement(8182..>8295)
                     /locus_tag="AC16JC_00955"
     CDS             complement(8182..>8295)
                     /locus_tag="AC16JC_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008208458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HIR5163293.1"
                     /translation="VHFYECEGCGRLLQPRVGDCCVFCSYGSEKCPPKQIG"
BASE COUNT         1271 a         2773 c         2887 g         1364 t
ORIGIN      
        1 tcgatcccga gcaagctgca ggtcttctgg tcggcgcatc ccgtggcctc gggcggcgcc
       61 ggcctcgtct atctcgcgct gttcgttctg gtcgggctct attggaagag gggcgcacga
      121 tgagagcggt tgccggtgca atggttctgg tcactgccgc gactggtccg ctggcggccc
      181 aacgggtgtc gcgcatgccg gtggaggcgg ccaacctggt ggtccaggta ctggttaccg
      241 gaggggatga cgaggaggtc ggggccggga tcgtggtcgc gtcgtccaat cgcatcgtca
      301 tcgcgaccgc ggcccacgtg gtccgagcgg cccagcaggg cggaagcatc cgggtcgtgt
      361 tccagttcgc ccgcaacgac accgtcgcag cgaaaatcga acaactggac cgtgagctcg
      421 acctggcggt gctgagcctg gcgcgtgatg gacgctcttc cattcaattc gcgttcgacc
      481 ggggaggaga ccccggagcg ctcaagatcg gtaccctcgt ggtcccactg gggtgccccg
      541 agcggcgttg ttgggagcct ccggtgagcg gtgaccgcgt gatcagtgcg agcgctcgcc
      601 gagtgcgctt cgagtcgttc ttcgtgagcc cggggagttc ggggggcgcg ctgttcaccc
      661 ggcagtggga ggtggtgggg ctggtgaccg aaaagaacac ccaggacggc gtcgcggtcg
      721 gcatgagcga ggtggcggac cggctgagaa agtggggcta caccgtccag ctgcgtccgg
      781 cgagcatccc ccgggccggg taccccacac ggctcagtct ggtcgggttg gctccgacct
      841 cgggcgccct gacgcaatcg ggccggtggc ccagcgggcg actcgctctg ttgctccggg
      901 gtcaggagcg gatcgggtgg cacctgacgg ctatccgtct cgcgccccag gacctgaagg
      961 tgaccggagg catggtgggg accagcgtct cgctccgggc cggacgactc gggatcgcgc
     1021 cgttcgggga ggtcggggtc gcccgggtga acggacgctt caatgccggg agctacttcg
     1081 tccagaccgc gagtggcacc gtggccgtcc ccgtatggca gacggtgacc aacgatgtga
     1141 tcggtgtcgg cgtcggggcg gacgtggagc tcctggtcgc cccccacgtc gccgtctcgg
     1201 gcgtcgtggg acactggagc ttccgcaccg atacgccgct gccgtcgatt cccgacgtct
     1261 tcgtcggggc gggcctcaag ctcgcgttct aaccctgaga ccccgtccat tcatgacgag
     1321 tcgatcgccg cacgtgagag ctatcaaggc gctgctcgcc ggagcgctcc tgaccacctg
     1381 caccaacgaa ctggcgccca ccggcgcgat cgtggtgcgc ctcgagaagg gcgctgcctg
     1441 gcccgacacc ctggccgttg cggagatcgc ctcgctggcc gtctccatca cgggcccgga
     1501 ccagcaggta gtcaccggcg tgacattggc gtggggctcc accgattcct cggtggtgac
     1561 cgtgaccgac gcagcggcag ccctgcacgg catcgtgagc agccggcgta ccggggaggc
     1621 cacgatcgtg gtgcgggtca cccagaccgg gttcgagccg gtcgagctgc gggcgccggt
     1681 cgtcgtccgg ccacggggcg ccgattcgtt gctctccgtc ggcgacgtcg acacgatcgg
     1741 cctggcgctg cagcgcgtgg acccggcctt ccttacgggc gccaccgtgg cctggggctc
     1801 gagtgatcct tccgtcgtcg gggtctccgc cctggccgcg gattccaccc gggccgtcat
     1861 caccgggcgg ttcagcggca cggcgcaggt caccgcgacg atccagagcc cgctcggtcg
     1921 ctcgaccttc cagctgccgg tggccgtctt cccgctcgag atcagcgagc tgccggcatg
     1981 ggcgcccacg gtgaaccgga ccaacaccgc tgcgttcgcg gtgcaggtga aggacgcgct
     2041 cggcaggatc aggcccggag tcaaggtgca gtggcactcc accaacgaga cggcgttcac
     2101 ggtcgattcg accggtaccg tgctggggaa gacccgcggg ggtggcgagc tggtggccag
     2161 cgtcggagtc gcgccgtttc aggtcgtcga acatcgcgcg ccactccagg tgaccgaaaa
     2221 gtggaaggcc gtgagcgccg ggggcgacca tagctgcgcg atcgcggcgc tggacggcac
     2281 gggctactgc tggggcagca acgcccaggg tcagctgggg ctcggcttcg atgcgagcgc
     2341 gcttggccag acctccctgc cgcgcgccgt cgccacgtcg cacaagttca cggagctcga
     2401 ggccggcgcg gcgcacagct gcgggcgcga ggggttccag accctgctct gttggggtgc
     2461 gagggatcgc ggggcactgg gcgacggcgt gtgcatcccc ggtgggatgg gttccacctg
     2521 cttcccctcg gcggaatcgc ccgtgaccgt cgtgagcggt ggcatcgtga gcggcgtgca
     2581 ggtccgcctg gatcggctga tcgtcggcgg cacgttcagt tgtatcgtca ccgtcaacgg
     2641 cgggtcgggg agtttcgcca gccgagactt gcgctgttgg ggcacagcgg acgacttcgg
     2701 ccggggaata tccttctccg ggtccggatt tgccgactcg gcggtaatcc tgcagccggg
     2761 cctctcgggg gacgccgaca tcaccgacgt ctcgaccggc ggcgcccatc tctgcaccag
     2821 gaccgacgat atctggtggg tccggtgcat gggcatcaac gaccacgcgc agctcggtga
     2881 cgggaccgtg ggcaacgatc cgctcccttc ctggctcccg aaggacttca cgatcgtggg
     2941 cggggatccc gtcaatccgg gtggggatgg ctatcccacg tcgggcctca gtgccggcgg
     3001 cagccacacg tgcgcgctcg atgcggtcgg ggtgctgtgc tggggctcga acgcgagcgg
     3061 ccagctgggg tctgccgtgg cgggcgatgc ggtctatccc acccgcgcgg cgctcccggt
     3121 ccaggcggtt gtgctgtcgg ccggcggcga tcacacctgc gccctcgtga ccggtggcga
     3181 cgtgtggtgc tggggctcca actcgcacgg ccagctcggg cgcggcacca tcggcggaag
     3241 cagctcggtg ccggcgccgg tgagcggtgg gctgacgttc gtgtcgatca gtgccggtgg
     3301 cgcgcacacc tgcggcgtga cggccgacgg atcactgtac tgctggggga acaactccag
     3361 tggtcagctc ggcgatggca cgcagaccga ttgggggacg cccgttcggg tcgtggaatc
     3421 gccgcagtag agagtcgctg cgccgaccgg gactcgagcc tggaccggcg gaggaccggg
     3481 aggaatcagg cgcctaacgg ggtcgcttcc cgcggtatgg ataccgccgg tttcaccggt
     3541 tctcgcctgt gtttcgatga cacaaggacc ccacgtcttg tagcgtgagc gcgacacgac
     3601 gctcacggtc gatgatcttt gtgccgatcc tggttgccgg acgatcgcga gagcgctgtg
     3661 ccggtcccgc ttgccgtgcc ggaaatcgcc gctccccgcg aggaccggtc caccgctgcg
     3721 atccatggaa caatgtcgag gttcaggcac gaagtgtgca ttctgcccgt caggcgacgc
     3781 tggcgcggat cggcggcgcg cagtgcccgg aatccgcggt catcaaccgc gtggaaagtc
     3841 gtgtggcaat gaagtgccgg cgcggattca ccgtcatcga ggtcaccgtc gccatcgtcg
     3901 tgctgacggt ggggctgctc gggctcgtga cgacggcggc actggtgacg cggatgctcg
     3961 cgcgcggcca acgaacggcc atggccgcga ccttcgcgca gagccggctc gagcgcctgc
     4021 gatcggcagc atgcattccc gcccaacgcg cggacggcag cgatacgttg tcccgcggct
     4081 cgacctgggt ggccatcaac cgctgggcct tttccggctc ctccagcccg accacgttcc
     4141 gcgtacgcat cgtcaccata tccaagacgg accggggtgc cattcgggtc gacacctcgg
     4201 aaagctcgat cccgtgccgc tgacgccccc tcgcccggcc cacgcggatc gcggcatgac
     4261 gctggtcgag atcctggtcg ccctgatgat gctgggcgtc gtgtcgggcg ccgtctaccg
     4321 ggtgctcctg aacaaccagc ggatctacca ggcacagacg cagcacatcg aactggaaca
     4381 gaacatccgt gcggcggtcg ccgtactgcc ggccgtgctg cgggaaatcg atgccaccga
     4441 cggcgacatc gtggcgatgt cgtccaccgc gctcaccatc cggagtatgc gctggctggg
     4501 tttcatctgc atgccgcccg ccctgggtgg cgcggtcgtc ggcaacacca tgaccctgcg
     4561 ggcgcagccg ttctacggct cgcgtggcct cacgatcccc accgattcga tgttcatcta
     4621 ctacgacggc aaccaggcca cgcgcaatga cgatgattgg gcgctggccc gccccacgga
     4681 cgtggcgaac ggcctctgcc cagacggcca gccggggctc gtcgtcacca tgagctggac
     4741 gctcgcgctc cccaacctgg ccggagggat cccgcccggg tcgccggtgc gtggcttcga
     4801 gacggtgacc tatcagctgt accagccgcc gggcgacagc ctctggtacg tgggcctcca
     4861 acccgccggc gagacgatcc agccgatcat cgggccggtg ctcccaggcg gcggcctgcg
     4921 cttcgcatac ttcgactcta ccggcaccgt gacgacgatc cccgggcgcg tcgcgcgcat
     4981 cgacgtcacg gtccgtggac gcacgtccca ggcggtgcag aacgccgcgg gcgccggcaa
     5041 gtccgtggcc gtggacagtg tcgtgaccag cgtggcattg cggaacaacc gacgctactg
     5101 agcgcagcgg cgagcgggac gccgaggagc gggccgatga aacggatctt gcgggacgag
     5161 cgggggatgg ccctggcgat cgcgatcttc gcgctgatcg tcgttggcgc ccttgtcgcg
     5221 ggcgcgttct tcgccggcac ccaggaacaa cgcgtcggag tgaattccag acgtgtgcaa
     5281 cagtccctcg gcgtcgcgga ggcggggatc gctgaacgcc tcgccacctg gcgccccgat
     5341 tcgctcaacc agcggggcat ctaccccgcc gattccgcgc ccatcgttct gacgccggcg
     5401 ccattcggca ccggctccta cggcggcgtc gtgtacaagc tgaccccgaa cctgttcctg
     5461 ctcgatgtgt cgggctccga ctgggccagc cgcgccggcc gcacgcaggg tgggagcggc
     5521 gcgcgccagc gggtcgggct gctgacacgg ctcgcccccg tccagttcga catccaggca
     5581 tccctcacca ctcaggggac cgtgaatctg aaaggcacgg cgacggtgga cggcaacgac
     5641 cagaacccca cggggtggct cgactgcggc ccactggcgg aggcgaaagc cggtatccgc
     5701 gtagagcccg gagcctcgat cgattcgggt gggagcgcga gcgtctcggg cgacccaaat
     5761 gtgtttcgcg acccgaccct gaccgactcg acgttcacgc gcttctcggg catcacctac
     5821 gccgagctgg cggcggaggc gaccgtgaag ctcccgggcg gcaactacaa agcgcagccc
     5881 gccatcctcg acggcaagtg cgacacacgc gatcaattga attggggcga cggccagaac
     5941 ccggcctcgg cctgcgggtc gtactttccc accgtccaca tcgccggctc cgccacgctg
     6001 aacggcgttc agggtcaggg cgtcttgctg gtggacggcg acctcacggt ggacggtggc
     6061 tttgaatggt tcggcatcgc gatcgtccag gggcagttcc agaccggcgg cggcggcaag
     6121 acgccagcgc acttctgggg cgccattctg gctcggaacg ccgatctctc cgtgcaggac
     6181 atggccggcc acgcgaccgt gaactactcg agctgcagta ttcgcaaagc gctgcagggg
     6241 acgagcaagc cgagcatgct tcggtcgcgc ggctggatac agctgtactg agcggcaagg
     6301 ttcttccggc ctccctcttc acacgacttt catgtcgtct taacgaaacg cacgacattt
     6361 ccacgtcatt gtgcgagtcg tgccgcgcgg ccgcccccgc cgacggcccg gcgctgcctc
     6421 caggattccg cccactccca ggaggagcca tgagcagacg actgcagcag gccgccgttg
     6481 tcttcgtcgt cgtcgtcgcc gccgctcagt tcattcgccc caagcgcgcg aaccccccga
     6541 tcgatgccag ccgcacgatc caggcgagcg tggggacggc gagcggattg agcgccgtcc
     6601 tggatcgcgc gtgcagcgaa tgccattcga acgcgaccgt gtggccgtgg tacagcaaca
     6661 tcgcgcctgc gtcctggctc atggcgtacg gcgtgaagcg cgggcgcgcg gcggtcaact
     6721 actcggagtg ggccgggtat ccgccggagc ggcaacggga gctgctcgtc gcctcgtgcc
     6781 aggacgcatc gaccggcaag atgcccggga gcctctacac gctgctgtat tccgagacgc
     6841 ggctctcgcc ccaggacgtc gagacgatct gtgcggcgtc gcgcgaggtc gcaagggaga
     6901 agccatgacg acaccaaccc tcgagacgaa ggtccacgac tttctggccc agaagcgcat
     6961 cgcggtggct ggcgtgtcgc gcgacaagag ccaccacccg gccggcaatc tgatctatga
     7021 gcggttgaag cggaccgggc acgacgtctt tgcggtcaac ccgaacatgc cgagcttcga
     7081 aggcgatcgc tgctatcccg acctgcaatc gatcccgggc ggggtcgatg gcgtggtgat
     7141 catcacgcga cccgagacca cgcagcagat cgtgcgcgac tgcggcaccg ccggggtccg
     7201 ccgcgtgtgg atgcaccaat ccatggcgaa gggatcgagt gtctcgccgg acgcggtcga
     7261 gtactgccgg caacacgata tcagcgtgat cgccggtgcg tgcccgatga tgttcggccc
     7321 gggcgtcgac ttcggacaca cgtgcatgcg gtggatcatg aaactcaccg ggggactacc
     7381 cacatgacac ggcgccaggt tgcctggatg ctcatcctca tcgtcgacgt gggatacatc
     7441 gcgtggggcg cgatggcggc cgcatctccg gatcacctgc tcaggggacc cggtggcaag
     7501 ccgatcctgg ccgccgccta tgaaggctac tcccatgcct cctggtcgca gctcgtaggc
     7561 acgtctccgt tgacagccgg atacatgctc gtgctctaca ggatgtacgg catctattgc
     7621 gccgtgttcg ggctgttggc gagcgccatc gcggtcacgg ccttccgtcg cggtgaggca
     7681 tgggcgtggt gggcgctgct cgtcggcaac acgatcgcgc tcgtgtcggc catgcggatg
     7741 gactggatcg tgaacgccat cgggccgttc gagctgacgg aatacctcgg tctcgccctg
     7801 gtctggggag cgctcgccgt caccgctcca ttccgggcag cgggacagcc ggttcgggcg
     7861 ggcggctgac gcggctcgcg cgaccggcgc cgctgctccc aggaacccat gaagctcgag
     7921 gtcatctccc gacacccgcg cgagagccgg catccgacgc cgtacgacat ccaagacgcc
     7981 ggtctggccc gtggcataga cgccgacgag cacgtcgttc tgatcccgga tgtccgcgta
     8041 gacgggatgg gacagatagc gcgttttggc tggccgttcc agttgtcgtc caccgccgcg
     8101 ggatcaccga tcgtgaccag ccgttcgcgc gagccccctg gtcgtgaatc atgggatgtg
     8161 gttgaacggc cctgagaact cttatccaat ctgcttcggt ggacacttct cggacccgta
     8221 cgagcagaac acgcagcagt cgccgactct tggctggaga agccggccgc acccctcgca
     8281 ttcgtagaag tgcac
//
LOCUS       DBNGZW010000039         2024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_685521, whole genome shotgun sequence.
ACCESSION   DBNGZW010000039 DBNGZW010000000
VERSION     DBNGZW010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_685521"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="AC16JC_00960"
     CDS             complement(<1..279)
                     /locus_tag="AC16JC_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5163294.1"
                     /translation="MDLSGKLCVVSGATEGIGRAIAFALGRSRAAVAICARTASAVEQ
                     AVAELRAAGIDAIGRPCDVSDPAAVAAFAGFVDRERGDAAVLVNNAGVG"
     gene            complement(263..673)
                     /locus_tag="AC16JC_00965"
     CDS             complement(263..673)
                     /locus_tag="AC16JC_00965"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020157052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HIR5163295.1"
                     /translation="MTAGPIPLGATGTKSVVVTRDLTVAHYHPGLPEAYGTPMMIYLM
                     EVAAGEAIAPFLPEGWASVGVGVSVEHLAATPIGRTVTATATVTAVDERTVTFAVTAH
                     DGIEPIGRGTHVRAPIELARFNTRLAAKANGPLR"
     gene            complement(670..1017)
                     /locus_tag="AC16JC_00970"
     CDS             complement(670..1017)
                     /locus_tag="AC16JC_00970"
                     /inference="COORDINATES: protein motif:HMM:NF015893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HIR5163296.1"
                     /translation="MEVQIFGVRKSADTRAALRFFAERRIRTHFVDLNEREPSPRELE
                     RFARKFGVQALVDRGARRFAELGLGASQLSDARWLEKLAVEPLLLRLPLVRWQNEVTI
                     GDAAEVWKAWCAK"
     gene            complement(1017..1697)
                     /locus_tag="AC16JC_00975"
     CDS             complement(1017..1697)
                     /locus_tag="AC16JC_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163297.1"
                     /translation="MTDRRRFLESLALSGAALALGACASATTTGQTPAPAPVPPVTKD
                     GPWDNGWLDQLTAKHKQVFDISAYADGGGLFYAKNYFNAHRDAFGTEYPNVQAVLGIH
                     GPAYPIVFADPVWEKFHFGKRGKIKDPRTKTWAVRNVMWQPREGEEMYEYSVNALQAR
                     GGKFIFCNNVLRFLTRTLARETGVPFETMRAELIAGLLPNVTVVPAMVVALGMAQERG
                     CAYVYAGG"
     gene            complement(1694..>2024)
                     /locus_tag="AC16JC_00980"
     CDS             complement(1694..>2024)
                     /locus_tag="AC16JC_00980"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR5163298.1"
                     /translation="TSVEDLVRWDRALYPGELLDQATLRLATTPPILPADSTQYGFGW
                     FVDTYRNERRWRHTGETSGFRNAIQRYPDRRFTVIVLTNRNGGDPAALAERITDLLLF
                     STAPERP"
BASE COUNT          303 a          715 c          698 g          308 t
ORIGIN      
        1 cccgaccccg gcgttgttca cgagcactgc cgcgtccccg cgctcacggt ccacgaagcc
       61 ggcgaacgcg gcgaccgccg cgggatccga cacgtcgcag ggacggccga tcgcgtcgat
      121 gccggccgca cgcagctcgg ccacggcctg ctccaccgcg gacgccgttc gcgcgcagat
      181 cgcaacggcg gcgcgcgagc ggcccagcgc aaaggcgatg gcgcggccga tcccttcggt
      241 cgcaccgctg acgacacaga gtttaccgga gaggtccatt ggctttcgcc gccagtcgcg
      301 tgttgaatcg ggccagctcg atcggcgccc ggacatgggt cccgcggccg atcggctcga
      361 tgccgtcgtg cgccgtgacg gcgaaggtga cggtgcgctc gtccaccgcg gtgaccgtcg
      421 cggtcgcggt gacggtccgg ccgatcgggg tcgcggccag gtgctccacg ctgacgccga
      481 cgcctaccga cgcccacccc tcggggagga acggcgcaat cgcttccccg gcggcgactt
      541 ccatcaggta gatcatcatc ggcgtgccgt aggcttccgg caggccgggg tgatagtgcg
      601 cgacggtcag gtcgcgggtg accacgacgg atttcgtgcc cgtggcgccg agggggatcg
      661 gcccggcggt cacttggcac accacgcctt ccagacctcg gccgcgtccc cgatggtgac
      721 ctcgttctgc cagcgaacca gcggcagccg cagcagcagc ggctcgacgg cgagcttctc
      781 gagccagcgg gcgtcggaga gctgcgaggc gccaagtccc agctccgcga accgcctcgc
      841 gccgcggtct accagcgcct gaacgccgaa tttccgcgcg aagcgctcca gctcgcgcgg
      901 gctgggctcc cgctcgttca ggtccacgaa gtgcgtccgg atgcgacgct ccgcgaagaa
      961 gcgcagcgcc gcgcgcgtgt cggcgctctt cctgacgccg aagatctgga cctccatcag
     1021 ccgccggcgt acacgtaggc gcagccgcgc tcctgggcca tccccagcgc caccaccatc
     1081 gccgggacga cggtgacgtt gggcagcaat cccgcgatga gctcggcgcg catcgtctcg
     1141 aacggcacgc cggtctcgcg cgccagggtc cgcgtcagga accgcaacac gttgttgcag
     1201 aagatgaact tgccgccccg ggcctggagc gcgttcacgg agtactcgta catctcctcg
     1261 ccctcgcgcg gctgccacat gacgttgcgc accgcccacg tcttggtgcg cgggtccttg
     1321 atcttgccgc gtttgccgaa atgaaacttc tcccacaccg gatcggcaaa cacgatcggg
     1381 taggcgggtc cgtggatgcc cagcaccgcc tgcacgttcg ggtattccgt gccgaacgcg
     1441 tcgcggtgcg cattgaagta gttcttggcg tagaacagcc cgcccccgtc cgcataggcc
     1501 gagatgtcga agacctgttt gtgcttcgcc gtgagctgat cgagccagcc gttgtcccac
     1561 ggtccgtcct tcgtgacggg cgggacgggg gccggagccg gggtctggcc cgtggtcgtc
     1621 gcgctggcgc aggctcccag cgcgagggcg gccccgctca gggcgaggct ctcgaggaat
     1681 ctccgacggt cggtcatgga cgctccggtg cggttgagaa caggagaagg tcggtgatgc
     1741 gctcggcgag cgccgccgga tcaccgccgt tgcggttggt caggacgatc accgtgaaac
     1801 ggcgatcggg gtagcgctgg atcgcattgc ggaagccgct cgtctccccg gtgtgccgcc
     1861 accgccgctc gttgcggtaa gtatccacga accagccgaa gccgtactgg gtcgaatcgg
     1921 ccggcaggat gggcggagtg gtggccagcc gcagcgtggc ttgatccagg agctcgcccg
     1981 gatacagcgc ccgatcccac cgcaccaggt cctcgaccga cgtg
//
LOCUS       DBNGZW010000040         3141 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1572266, whole genome shotgun sequence.
ACCESSION   DBNGZW010000040 DBNGZW010000000
VERSION     DBNGZW010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1572266"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2399)
                     /locus_tag="AC16JC_00985"
     CDS             complement(<1..2399)
                     /locus_tag="AC16JC_00985"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5163299.1"
                     /translation="MEAVLQDIRYAVRQLARSPAFTAVAVLTLALGIGANTALFSVID
                     ALMLRHLPVDHPEELVTLTATRPGDADEAFSYAAYTLFREEAARFADVVAVTRTDRAS
                     IAVAGEPELVYRKSVSGNYFSALHVPAALGRTLLAEDDRSPGGQPVAVLNYAYWTRRF
                     GGDPTILGRTFTFKETAFTIVGVAPRGFFGETVGEAPDVWTPLTVQPGAPAWLWRGHS
                     TTWLRLLARRRPGVTLAQARAVLDPLFERVTDEIAGETSDPQFRQQVRESRLGVANGS
                     HGLSELRRRFSRPLGILMVVVGLVLLIACANLANLLLARAAARRREMGIRLAIGAGRL
                     RLVRQLLAESLVLAGVGGGLAVMLANWSGAILVALPSRGPAPIPLDVGPDARVLAFTA
                     AVALVTAIAFGLAPALRAARLDLLSAMKEPSGGGRSTARIPLGRALVIAQMALSVLLL
                     VAAALFIKSLGKIRSIETGFEPDRVLLVKLAPPAVRDSLPEAELRRLYGLLLERAKSV
                     PGVRAASLSFSGVFSRGTWGNQITVEGQTAPPGVVLRTLANAVSPGYFQVTGIPVLRG
                     RTFVETDDERAPRVAVVNQAFARRFFGGADPIGRRVGLVPAQDPMEIVGLVQDAKYAD
                     LREAPAPMLYVPYTQYPSQLRELEVRTAGDLVAVAAGLRRELAAVDPRLPVLDLLRMN
                     EQIDASIAPERLIAKLSSVFGLLALALASVGLYGVMSYVVAQRTAEVGIRIALGADAG
                     AVLRLVLRDMLPLVLGGIVIGVPAALVGGRFISSLLYGLSPRDPIAITTAIVILSTVA
                     VV"
     gene            complement(2476..>3141)
                     /locus_tag="AC16JC_00990"
     CDS             complement(2476..>3141)
                     /locus_tag="AC16JC_00990"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR5163300.1"
                     /translation="QVSWADPTAHPEIVGVVGDVRTSALDAEIHPMIYYVEAQAPTGE
                     LTLVVRHSGDAAALVSAVRGAVRELDRDVPLTDVASMSTRLTRSMSDRRYPMLLLSGF
                     AVLAVLLAAVGLYGLLSYAVSRRVQEIGVRMALGASRGTVLGMVMKNGVRLTLVGVAL
                     GAAGGVLAARALGRLLYGISPTDPVTFVAVAVLLVAVALLASYVPAARATRVDPVVAL
                     RTD"
BASE COUNT          534 a         1100 c         1089 g          418 t
ORIGIN      
        1 acgaccgcaa cggttgaaag aatcacgatg gcggtggtga tcgcgatggg atcacgtggg
       61 ctcaaaccgt aaagcaagct cgaaatgaag cgaccgccga cgagcgctgc gggtactccg
      121 atgacgatcc cgcccagcac cagggggagc atgtcgcgta acaccagccg gagcacggcg
      181 ccggcgtccg caccgagcgc tatccgaatg ccgacctccg ccgtgcgttg cgccacgaca
      241 tagctcatga ccccgtacag cccgaccgac gcgagcgcga gggcgagcag cccgaagacg
      301 ctcgagagct tcgcgatcag tcgctccggc gcgatcgagg cgtcgatctg ctcgttcatg
      361 cggagcaggt cgagcacggg cagcctcggg tccactgcgg caagctcgcg gcgcagcccg
      421 gccgcgacgg cgacgaggtc gcccgccgtc cggacctcca gttctctgag ctggctggga
      481 tactgggtgt acgggacgta cagcatggga gccggcgctt ccctcagatc cgcgtacttc
      541 gcgtcctgca cgagcccgac gatctccatc ggatcctgcg cgggtacgag gccgacacgc
      601 ctgccgatcg ggtcggctcc gccgaagaac cgccgcgcga aggcctggtt cacgaccgcg
      661 accctgggcg cccgctcgtc gtcggtctca acgaaagtgc gacctctgag caccggaatg
      721 cccgtgacct ggaagtatcc cgggctgacg gcgttggcga gggtccggag caccacgccg
      781 ggaggcgccg tttggccttc caccgtgatc tggttccccc aggtgcctcg cgagaagacg
      841 ccggagaagg acaggctggc cgcgcgaacg cccggcacag acttggcccg ctcgaggagg
      901 agcccgtaga gccggcggag ctccgcctcc ggcagggagt cgcgaactgc ggggggcgcg
      961 agcttgacga gcagcactcg atccggctcg aaccccgtct cgatgctgcg aatcttcccg
     1021 aggctcttga tgaagagcgc cgcggcgacc agcagcagca ccgagagcgc catctgcgcg
     1081 atcaccaggg cgcggccgag cggaatgcgc gccgtgcttc gtcctccgcc ggatggttcc
     1141 ttcatcgccg acaacaggtc gaggcgcgcc gctcgcagcg ccggcgcgag tccgaaggcg
     1201 atcgccgtga cgagagccac ggcggccgtg aaggcgagca cgcgagcgtc cggacccacg
     1261 tcgaggggaa tcggcgccgg cccgcgtgac gggagggcga ccaggatggc cccgctccag
     1321 ttcgccagca tcaccgccag ccccccgccg accccggcca ggaccagcga ttcggcgagc
     1381 agctgtcgca cgagtcgcag gcggcccgct ccgatcgcga gccgtatccc catctcgcgc
     1441 cgccgcgcag ccgcccgcgc cagcagcagg ttcgccaggt tggcgcaggc gatcaggagc
     1501 acgagcccga cgacgaccat gagaatcccg agcgggcggg agaagcgtcg ccgcagctcg
     1561 gaaagaccgt ggcttccgtt cgcgacgccg aggcggctct cgcggacctg ttgccgaaac
     1621 tgcgggtcgc tcgtctcgcc ggcgatctcg tccgtgacgc gctcgaagag cgggtcgagg
     1681 acggcgcgtg cctgcgcgag tgtgacccca ggacggcgcc gcgcgagcag ccggagccac
     1741 gtggtgctgt gtcccctcca cagccaggcg ggtgcgccgg gttgcacggt gagcggcgtc
     1801 catacgtccg gcgcctcgcc cacggtctcg ccgaagaatc cccgaggcgc caccccgacg
     1861 atcgtgaacg cggtctcctt gaacgtgaag gtgcgcccca ggatcgtcgg gtcgcctccg
     1921 aagcggcggg tccagtaagc gtagttgaga acggcgacgg gctggccgcc cggtgagcga
     1981 tcgtcctcgg cgaggagggt ccgtcccaaa gccgccggca cgtgcagcgc cgagaagtag
     2041 tttccggaga cggatttgcg atacaccagc tccggctcac ctgcgaccgc gatgctggct
     2101 cggtcggtgc gagtcacggc aaccacgtct gcgaaacgcg cggcctcttc ccggaacagg
     2161 gtgtaggcgg cgtaggagaa cgcttcgtcg gcgtcccccg gtctggtggc cgtcaacgta
     2221 accagctctt ccggatggtc gaccgggagg tgtcgcagca tcagggcgtc gatcacgctg
     2281 aagagcgccg tgttcgctcc gatgccgagc gcgagggtca gcacggcaac ggcggtgaac
     2341 gccgggctcc tggcgagctg gcggaccgcg tagcggatgt cttgcagcac ggcctccatt
     2401 gtgcctccag aagcaggtgt caggtgccgg gtgtcaggtg ccagaaacga tcccctgaca
     2461 cccgactcct ggcacctaat cagttcttag cgccaccacc ggatccaccc gcgtcgcccg
     2521 cgcagccggg acgtaactgg ccagcagcgc cacggccacg agcaggacgg ccaccgccac
     2581 gaaggtgacg ggatccgtcg ggctgatgcc gtagaggaga cggcccagcg ctcgtgcggc
     2641 cagcacgccg cccgcggcgc cgagcgccac gcctacgagc gtcaggcgta cgccgttctt
     2701 catgaccatg ccgaggacgg tgccacggct tgctccgagc gccatgcgca cgccgatctc
     2761 ctgaacgcgg cgcgagacgg cgtacgacag cagaccgtac agtccgacgg cggcgagcag
     2821 caccgccagc accgcgaacc ccgacagcag cagcatcggg tagcggcgat ccgacatcga
     2881 ccgcgtcagc cgggtggaca tcgacgcgac atccgtgagc ggcacgtcgc ggtcgagttc
     2941 gcggacggcg cctcgtacgg cggagaccag cgcggcggca tcgccgctgt ggcgcaccac
     3001 cagcgtcagc tccccggtcg gcgcctgcgc ctcgacgtag tagatcatgg ggtggatttc
     3061 cgcatcgagg gcgctggtgc gcacgtcgcc gaccacgccc acgatttccg gatgagccgt
     3121 cggatcagcc cagctcacct g
//
LOCUS       DBNGZW010000041         3151 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1503646, whole genome shotgun sequence.
ACCESSION   DBNGZW010000041 DBNGZW010000000
VERSION     DBNGZW010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1503646"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="AC16JC_00995"
     CDS             <1..275
                     /locus_tag="AC16JC_00995"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5163301.1"
                     /translation="DLDRKLVAPELALPTENADRETAERRLAESRAALLAAIESGDGL
                     ALGAIRHTHLRFGELDLYQWILFVAEHEARHTAQVREVARQLVPAS"
     gene            319..978
                     /locus_tag="AC16JC_01000"
     CDS             319..978
                     /locus_tag="AC16JC_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163302.1"
                     /translation="MIRRSLTTLSLLALSAAPLVAQGANDPTHKVEGGALPAGWMGRT
                     DKPTDKLTDAKFVAMGSGFHATSGPAAIYWNPKNVVTGPFTATTTVTQTKAPTHPEAY
                     GIFFMGKDLDTPQQNYAYFLVRGDGKYMVNHRAGAELHKIIPWTDNAAVNKADASGKA
                     TNKLTVDATKADSVRLLVNDKQVAALPMNHLGKTDGLVGLRVNHNLDVHIGDLTITPK
                     K"
     gene            982..2433
                     /locus_tag="AC16JC_01005"
     CDS             982..2433
                     /locus_tag="AC16JC_01005"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5163303.1"
                     /translation="MTSRGAPEQRSAARSAGAPSLDRALGLRDLVLFNLVAVLGLRWL
                     ATAAKAGPSDISLWLLAALFFFVPQGLVVTELSSRYPEEGGIYQWTKRALGETHGYVC
                     GWCYWINNVLYYPNLLISAAAMATYAIGRGDSPMASSWTYVLPATLSCLWLAAGLNIV
                     GVGTGKWLQNAGGIGTYVPGVMMVVLAIALVATGRPSANPITPHNVMPNLGTLQGLNL
                     WASIAFAFAGLELSSSMGGEVRDATRTLPRSVLVSAPLIAAAYVLGTWALLRIVPAGD
                     INIVSGLLQSISSGARALSPALWWLAPVAAAAYTLGNIGGVGAWLTAPARVAFTIGLD
                     RYFPPAFGRIHPTWKTPYVAILVQAALATAFLLVSVLGKGTTVERAYLIILDTQLLIY
                     FIPYVYLFISFLLLRRTDRSADTVRVPGGPLGARAIGVTGLVVTLLAMGIAMVPPDGE
                     TDPWLFELKVVGGALAFVALGGVVYWRGVRERR"
     gene            2590..>3151
                     /locus_tag="AC16JC_01010"
     CDS             2590..>3151
                     /locus_tag="AC16JC_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5163304.1"
                     /translation="MLLLLAALLQQAQPVAQSAPASPVVRIAIAPAKLVVAASDSIRL
                     TAVAYDVNGAPIEGAGLRFSGGAANAGIVDSTGWVIARGTGKITGAVMSIIPGYKPFI
                     HRFEVVVVPDAPSRIAIGALPATLVVGQRVHATAAVYSKADDVREGDGVRWSSSAPSI
                     VAVDDDGVVTATAPGRAAITATDAPAT"
BASE COUNT          475 a         1075 c         1087 g          514 t
ORIGIN      
        1 ctgacctcga ccgaaagctg gtggcgcccg agctcgccct gccgacggag aacgcggacc
       61 gcgagaccgc cgaacggcgg ctggccgaat cgcgcgcggc gctgctcgcc gcgatcgaga
      121 gcggggacgg gctcgcgctc ggcgcgatcc gtcatacgca cctgcggttc ggcgagctcg
      181 atctctacca gtggattctc ttcgtcgccg agcacgaggc gcggcacacc gcacaggtgc
      241 gcgaggtggc gcggcagctc gttccggcat cctgacgtca cgccgttcga accctcactc
      301 ttctggagac tcgccatcat gattcgtcgt tcgctcacca cgctctcgct cctcgcgctc
      361 tccgccgcac cgctcgtggc gcagggtgcc aacgatccca cgcacaaggt cgaaggcggc
      421 gcgctgcccg ccggctggat ggggcgcacc gacaagccga ccgacaagct gaccgacgcg
      481 aagttcgtcg cgatgggctc cggtttccac gcgacctccg gcccggcggc gatctactgg
      541 aacccgaaga acgtcgtgac gggcccgttc accgcgacga cgacggtgac gcagaccaag
      601 gcgccgacgc accccgaggc gtacggcatc ttcttcatgg gcaaggacct cgacacgccg
      661 cagcagaact acgcctactt cctcgtgcgc ggcgatggca agtacatggt caaccatcgg
      721 gcgggcgccg agctccacaa gatcatcccg tggacggaca acgcggccgt gaacaaggcg
      781 gacgccagcg gcaaggcgac gaacaagctc accgtggatg cgacgaaggc cgactcggtg
      841 cggctgctcg tgaacgacaa gcaggtcgcg gcgctgccga tgaaccatct cggcaagacc
      901 gacgggctcg tggggctgcg cgtgaaccac aacctggacg tgcacatcgg cgatctgacg
      961 atcacgccga agaagtaagc ggtgacctca cggggcgctc cggagcagcg gtcggccgcg
     1021 cgctccgcgg gcgccccttc gctcgatcgt gcgctcggac tccgcgacct ggtgctgttc
     1081 aacctggtcg cggtgctcgg gctgcggtgg ctcgccacgg cggccaaggc gggaccgagc
     1141 gacatctcgc tctggctact cgccgccctt ttcttcttcg tgccgcaggg gctcgtcgtc
     1201 accgagcttt cgtcgcggta tccggaagag ggagggatct accagtggac caagcgtgcg
     1261 ctcggcgaga cgcacggcta cgtctgcggc tggtgctact ggatcaacaa cgtcctgtac
     1321 tatcccaacc tgctgatctc ggcggcggcg atggcgacat atgccatcgg ccgtggtgac
     1381 agcccgatgg cgagcagctg gacgtacgtc ctccccgcga cgctctcgtg cctgtggctg
     1441 gcggcggggc tgaacatcgt gggcgtcggc acgggcaagt ggctgcagaa cgcgggggga
     1501 atcggcacgt acgtgccggg cgtgatgatg gtggtgctcg cgatcgccct ggtggcgacg
     1561 ggacgtccat cggcgaaccc gatcacaccg cacaacgtga tgccgaacct cggcacgctg
     1621 caggggctca acctgtgggc atcgatcgcc tttgcgttcg cggggctcga gctctcgagc
     1681 tcgatgggtg gcgaggtgcg tgatgcgaca cgaacgctgc cgcgctccgt cctcgtgtcg
     1741 gcgccgctga tcgccgcggc ctacgtgctg ggcacgtggg cactcctgcg gatcgtgccg
     1801 gcgggcgaca tcaacatcgt gtccggactg ttgcagagca tctcgagcgg cgcgcgggcg
     1861 ctgagcccag ccctgtggtg gctcgccccc gtcgccgcag cggcgtacac actgggaaac
     1921 atcggcgggg tcggcgcatg gctgacggcg ccggcacgcg tcgccttcac gatcgggctc
     1981 gatcgctact tcccgccggc cttcggccgc atccacccga cgtggaagac gccgtacgtg
     2041 gcgatcctcg ttcaggccgc gctcgcgacg gcgtttcttc tggtgtccgt gctggggaag
     2101 gggacgacgg tcgagcgggc gtatctgatc atcctcgaca cgcagctcct catctacttc
     2161 atcccgtacg tctacctgtt catctccttc ctgctcctgc gccgcaccga ccgctcggcg
     2221 gacacggtgc gcgtgcccgg cggcccgctc ggcgctcgcg cgatcggcgt gaccgggctg
     2281 gtggtcacgc tgctggcaat ggggatcgcc atggtaccac ccgacggcga gaccgatccg
     2341 tggctgttcg agctcaaggt cgtgggcggg gccctggcct tcgtggcgct gggtggcgtg
     2401 gtgtattgga gaggcgttcg agaacggcgg tgagtggata ctgcggtgag cggatactgc
     2461 ggtgaaggga tattgcggtg agctggtcca cgaacgcacg cttcacccgc tcgccacctc
     2521 acccgttcac cgcttcaccc gttcacggct tcacccgttc accgcttcac cccgtcaccg
     2581 ccgtttcaaa tgctcctcct cctcgccgcg ctgctgcagc aagcccaacc cgtggcgcag
     2641 agtgcacccg cgtcaccggt ggtgcgcatc gcgatcgcgc cggcgaagct cgtcgtcgcg
     2701 gcgagtgact cgattcggct gaccgccgtc gcgtacgatg tgaatggggc gccaatcgag
     2761 ggggccggcc tgcggttctc cggcggcgcg gcgaacgccg gcatcgtcga ttcgacggga
     2821 tgggtgatcg cgcgcggcac ggggaagatc acgggtgcgg tgatgtcgat catcccgggg
     2881 tacaagccgt tcatccaccg gttcgaggtc gtggtcgtcc ccgacgcacc gtcgcgcatc
     2941 gcgattggtg cgctgccggc gacgctggtg gtggggcagc gtgtgcacgc caccgccgcg
     3001 gtctactcga aagccgacga cgtgcgggag ggtgacggcg tgcgctggag ctcgagcgcg
     3061 ccgtcgatcg tcgcggtgga tgacgacggc gtcgtcaccg ccaccgcacc ggggcgcgcc
     3121 gcgatcacgg cgaccgacgc tcccgccacg g
//
LOCUS       DBNGZW010000042         3996 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1299106, whole genome shotgun sequence.
ACCESSION   DBNGZW010000042 DBNGZW010000000
VERSION     DBNGZW010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3996
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1299106"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..259
                     /locus_tag="AC16JC_01015"
     CDS             <1..259
                     /locus_tag="AC16JC_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163305.1"
                     /translation="AVGLGVTTSPGPAQLFHTTPTAEIMTRFPMALVPTFLVPLAFVL
                     HFVSLWQLLGRPWEGSATGALRRPSAHAAPLDASRVESGGS"
     gene            358..864
                     /locus_tag="AC16JC_01020"
     CDS             358..864
                     /locus_tag="AC16JC_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163306.1"
                     /translation="MLIVLRFFHIIAGIFWVGGALFAARFIAPSLKAAGPGAGPVLAE
                     LGKRRIPASMMGAALVNIASGIWLMVIDAGSAPGVWMRSGMGRTLGIGAALAIITVLL
                     GMTVAMPTTARMGRISAAAQKRGGPPTAEEAAELGRLQARSALIAMVASVLLFLAASA
                     MAVARYVP"
     gene            complement(1014..1562)
                     /locus_tag="AC16JC_01025"
     CDS             complement(1014..1562)
                     /locus_tag="AC16JC_01025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163307.1"
                     /translation="MMRRAAAIAAACLLALPPADGYLAATMERRLLIQIPAMLVLGAL
                     AGWRPAAQRPWRSGDLAALAFAVGAMLFWMIPRSLDLAARSRPADAALLLSVFAAGLA
                     LVRSLPRLSFVIPVALGLQATAMLMALGIVYLHFPGLICTAYTLAQQRVAGAGLVRAA
                     PVAWVLLWGWALRRLAVARSLP"
     gene            complement(1559..2167)
                     /locus_tag="AC16JC_01030"
     CDS             complement(1559..2167)
                     /locus_tag="AC16JC_01030"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR5163308.1"
                     /translation="MRRYLPAAAVLAGWITFAGWWTFGFRAFTTDSAALAAAGPLPRP
                     ASPLTFIDPAGDTTTLARYRGRYLLLTFMYLHCPGVCQLVTLRLQQARGELADAVPDR
                     VLFLSVSLDPERDDSAALEHHWRALGSGAGWVAGRLARPLDRRQLADLARLGVWVSRD
                     PSGRIDHSASTFLLDPAGTVVDVFRPEVPASALVAAVRRRLS"
     gene            complement(2183..3634)
                     /locus_tag="AC16JC_01035"
     CDS             complement(2183..3634)
                     /locus_tag="AC16JC_01035"
                     /inference="COORDINATES: protein motif:HMM:NF012343.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR5163309.1"
                     /translation="MTDSTAVAPLSAKRMAMVWAITVVVVFLVLVLLGLVMRLNQGAA
                     IAIPPDRFYAIMTLHGLGMAGTLFIGGVAAIWYRSLRYVRPSLTLLRWVYGLVLAGTV
                     GLIAATLLGRYGPGWYMLYPLPFIQMWPHWSTGLAVVSVLVLGVAWLLGQLDLLRAFA
                     ARYGVGRLLAWDYLTGASPADPLPPFILIATVSLLAGALTTVVGAVVLLLYLFQWFMP
                     ALHFDPLLLKNMMFLFGHTIVNITMYLGIALVYERLPEYTGRKWSTNRIVAIAWNATL
                     LFVLTAFFHHLYMDFAQPGAVQMIGQIASYLSAVPATAVTLFGAATQVYRADVRWRFA
                     PLAYYTGLVGWTVGGLAAVVDSTIAVNLFFHNTLWVPGHFHTYFLAGYVLMLLAAFHE
                     IAAPEAQRSAKAGLAMLLVGAYGFLVMFYVGGEQGVPRRYASYAAIPVEGLANTGRVL
                     ALVAAGFVVLVLLGLAAYGLSLLQGWRRSWTEA"
     gene            complement(3638..3781)
                     /locus_tag="AC16JC_01040"
     CDS             complement(3638..3781)
                     /locus_tag="AC16JC_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5163310.1"
                     /translation="MLGQTQAMPGYRNALTLTFPAPGTYRILCLEYCGLGHQTMFSSI
                     EAR"
     gene            complement(3782..>3996)
                     /locus_tag="AC16JC_01045"
     CDS             complement(3782..>3996)
                     /locus_tag="AC16JC_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5163311.1"
                     /translation="LPRTPYARSARESPAMTLTVDGVMWSWRVTGPDTSSGSAGPVIV
                     PVGKPVRFEVGALDVNHGFAVYRPDG"
BASE COUNT          690 a         1349 c         1439 g          518 t
ORIGIN      
        1 ggccgtcgga ctcggcgtga cgacgagtcc cgggccggcg cagctgttcc ataccacgcc
       61 caccgccgag atcatgacgc ggttcccgat ggccctggtg cccacgttcc tcgtgccgct
      121 ggccttcgtg ctccacttcg tgtcgctgtg gcagctgctg ggccgcccat gggagggctc
      181 ggcaacgggt gcactgcgac gcccctcggc acacgcagcg ccgctcgatg cctctcgtgt
      241 cgaatccggg ggcagctgat cgtcgccagg aagacgcgac tcaccgagac ccacggggta
      301 tcgggggacg agcggctatt taccttgcac gggccaccaa acacgggtga agcgaacatg
      361 ctgatcgtac tgcgattctt tcacatcatc gcgggaatct tctgggtcgg cggcgctctc
      421 tttgccgccc gattcatcgc gccttcgttg aaagcggcgg gtcccggtgc cggtccggtc
      481 ctggccgagc tgggcaagcg gcgcataccc gcgtccatga tgggtgcggc gctggtgaac
      541 atcgcttctg ggatctggct catggtgatc gatgccggga gcgcgcccgg cgtgtggatg
      601 cgctccggca tgggccggac gctcgggatc ggcgccgctc tggcgattat cacggtgctg
      661 ctgggcatga ccgtcgccat gcccacgacc gccaggatgg gccgcatttc ggccgcggct
      721 cagaaacggg gcggcccgcc aaccgctgaa gaggcggcgg agctggggcg gctgcaggcg
      781 cggtcggcgc tcatcgccat ggtcgcgtcc gtgctgctgt ttctggcggc gtcggcgatg
      841 gccgtggcgc gctacgtgcc gtagcgtcac agcatgagcg cgtctgccgt ttgggtacct
      901 ctggcccttg cccctcctcc tgtcgggctt cgtacctcac cccctgtccc cctctccgct
      961 taacgcggag agggggcaca tggccggagc gtccccccat cgggcggcgg cgactacggc
     1021 aatgatctcg cgactgcgag cctgcgcagc gcccaacccc agaggaggac ccaggcgacc
     1081 ggcgcagccc gcaccagccc cgccccggcg actcgctgct gcgcgagggt gtacgccgtg
     1141 cagatgagtc ccgggaagtg caggtagacg atgccgagcg ccatgagcat ggcggtcgcc
     1201 tgcagcccca gggccacggg aatgacgaac gacagccgcg gcaacgaccg cacgagcgcg
     1261 aggcccgcgg cgaacaccga caggagcagc gctgcgtcgg ccgggcggct ccgggcggcg
     1321 aggtccaggg atcggggaat catccagaac agcatcgccc cgacggcgaa cgccaacgcg
     1381 gcgaggtcgc cggagcgcca cggtctctgg gccgccggcc gccagccggc gagggcgccg
     1441 aggaccagca tagcgggaat ctggatcagc aggcggcgct ccatggtggc ggcgaggtag
     1501 ccgtcggcgg gcgggagcgc gagcaggcac gccgccgcga tggctgcggc gcggcgcatc
     1561 acgacagcct cctccgcacg gccgcgacca gtgcgctggc ggggacctcg gggcggaaca
     1621 catcgacgac ggtgccggcc gggtcgagga ggaaggtgga cgcggagtgg tcgatccggc
     1681 cgctcgggtc ccgcgacacc cagacgccca ggcgcgcgag gtcggccagc tgacgccggt
     1741 cgagcgggcg ggcgagtcga cccgcgaccc agcccgcccc ggagccgagg gcgcgccagt
     1801 gatgctcgag tgcggccgag tcgtcgcgct cggggtcgag gctcacgctc aggaacagca
     1861 cgcggtccgg gacggcgtcg gcgagctcgc ctcgtgcctg ctgcagccgc agggtcacga
     1921 gctggcagac gccggggcag tgcaggtaca tgaacgtgag cagcaggtat cggccccgat
     1981 agcgggccag cgtggtcgtg tcgccggcgg ggtcgatgaa ggtgagtggt gacgccggtc
     2041 gcggcagcgg ccccgcggcg gcgagggccg cgctgtcggt cgtgaacgcc cgaaacccga
     2101 acgtccacca gccggcgaac gtgatccagc cggccagcac cgccgcggcg ggaaggtagc
     2161 ggcgcatcct gctccccccg gctcaggcct ccgtccagct gcggcgccag ccctgcagga
     2221 gcgagaggcc ataggctgcc agacccagga ggaccagcac gacgaagccc gccgccacga
     2281 gcgccaggac cctgccggta ttggcgagcc cctcgacggg gatcgccgca taactggcat
     2341 agcgtcgcgg cacgccctgc tccccgccca cgtagaacat gaccagaaag ccgtaggccc
     2401 cgaccagcaa catcgccagg ccggccttcg ccgaccgttg cgcttcggga gccgcgatct
     2461 catggaacgc ggcgaggagc atcaggacgt atccggcgag gaagtaggtg tggaagtgcc
     2521 ccgggaccca gagcgtgttg tggaagaaga ggttcaccgc gatggtcgag tccacgactg
     2581 ccgcaagccc gccgaccgtc cagccgacga ggcccgtgta ataggcgagc ggcgcaaacc
     2641 gccagcgcac gtccgcccgg tacacctgcg tggcggcgcc gaacagcgtc accgccgtcg
     2701 ccggaacggc actgagatag gaggcgatct ggccgatcat ctgcacggca cccggctggg
     2761 cgaaatccat gtagaggtga tggaagaacg cggtcagcac gaagagcagc gtggcgttcc
     2821 acgcaatggc cacgatccgg ttggtcgacc acttgcgccc cgtgtactcg ggaagccgct
     2881 cgtacaccag cgcgatcccc aggtacatgg tgatgttgac gatcgtatga ccgaacagga
     2941 acatcatgtt cttcagcagc agcgggtcga agtggagcgc cggcatgaac cactggaaca
     3001 gatagagcaa caggacgacg gcgcccacca ccgtcgtcag cgccccggcg aggagcgaca
     3061 cggtggcgat gagaatgaac gggggaagcg ggtccgcggg tgacgcgccg gtgaggtagt
     3121 cccaggccag caggcggccc acgccgtagc gggcggcgaa cgcccgcaac agatcgagct
     3181 gaccgaggag ccacgccacg cccagcacca gaacggatac gaccgccagg cccgtggacc
     3241 aatggggcca catctggatg aagggcagcg gatacaacat gtaccagccc gggccgtagc
     3301 gtcccaggag ggtcgccgcg atcaggccca ccgtgccggc cagaaccagg ccgtagaccc
     3361 agcggaggag cgtgaggctc ggccggacgt agcgcagcga ccggtaccag atggccgcga
     3421 cgcctccgat gaacagcgtg ccggccattc ccaggccgtg cagcgtcatg atcgcgtaga
     3481 agcggtcggg agggatcgcg atcgccgcgc cctgattcag gcgcatcacc agcccgagga
     3541 gcaccagcac caggaagacc acgacgacgg tgatggccca gaccatcgcc atgcgcttgg
     3601 ccgaaagcgg tgcgactgcc gtggaatccg tcacgcgtca cctcgcttcg atcgaggaga
     3661 acatggtctg atgacccagc ccacagtact cgaggcacag gatgcggtag gtaccggggg
     3721 ccgggaaggt cagggtcagg gcgttgcggt agcccggcat ggcctgagtc tggccgagca
     3781 ttcacccatc gggccggtag acggcgaatc cgtggttcac gtcgagcgcg ccgacctcga
     3841 atcgtaccgg cttgccgacg ggaacgatca ccgggccggc cgagccggag gaggtgtccg
     3901 gtcccgtcac gcgccacgac cacatgacgc cgtcgaccgt gagcgtcatg gccggcgact
     3961 cgcgcgcgct gcgggcgtat ggcgttcggg ggagcg
//
LOCUS       DBNGZW010000043         2670 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_549322, whole genome shotgun sequence.
ACCESSION   DBNGZW010000043 DBNGZW010000000
VERSION     DBNGZW010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_549322"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1153)
                     /locus_tag="AC16JC_01050"
     CDS             complement(<1..1153)
                     /locus_tag="AC16JC_01050"
                     /EC_number="3.4.16.-"
                     /inference="COORDINATES: protein motif:HMM:NF012664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S10 family peptidase"
                     /protein_id="HIR5163312.1"
                     /translation="MRLGTIVLMGCALAAPARAQRPAAPPQPAAAAADRDTTAKAGPI
                     PKETSSVTEHTIRIGGQLVPYRASAATMLLTNDKDEPIGSLYYTAYTRSDARDPAQRP
                     LAFVYNGGPGSASAWLHMGAFGPRRIVTTDAAPTPPPPYQMVDNANSLIDVADLVFID
                     PVGTGFSKPVGKGTGKDFWGVDEDARSLAQFVSQYVSRNGRWSSPKYLIGESYGTTRS
                     AVLVNRLQRDGMTFNGMVLISSVLDFETLLFSPGHDLSYVLYLPSYAATAVYHKVIPA
                     PANLDAFLTEVRRYAMGDYADALAAGATLPADRKAAVAKHVAAYTGLSEDYLMKADLR
                     VNLRQFMAELQRSRGLVTGRLDSRFTGPLPDLLSETAPGDPQSSAVSGAF"
     gene            1277..2488
                     /locus_tag="AC16JC_01055"
     CDS             1277..2488
                     /locus_tag="AC16JC_01055"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BaiN/RdsA family NAD(P)/FAD-dependent
                     oxidoreductase"
                     /protein_id="HIR5163313.1"
                     /translation="MTRVAVIGGGAAGMMAAAFAAAGGAETLLLEHTPDGGRKILISG
                     GGRCNILPADLDETRFVTDSSPHTLRNIVRSWPLHEQRAFFERVLGQPLVEETESAKL
                     FPASQRARDVRDGLLAHAVARGARWVPSTTVSGLARAGDGWRIACKDERTLDADRVIV
                     ATGGLSVPKTGSDGRGLALLAELGHTIHEPYAALTPVQAEPAPFAALAGVSLTVSLVA
                     RDAERSARARGGFLFTHRGYSGPAVLDVSHVLVRSSGGARLIAQWTALDAEGWERVLR
                     GDGARGVVGVVRGEMPARLGDLLVRRAGIDPETKLSQLRRADRVRLVEGLARCELPWT
                     GHGGYAKAEVTGGGVSLAEIDPRTLESRKAPGLYVCGEVLDAFGPIGGYNFLWAWATG
                     RAAGLAAARGN"
     gene            2512..>2670
                     /locus_tag="AC16JC_01060"
     CDS             2512..>2670
                     /locus_tag="AC16JC_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5163314.1"
                     /translation="MPLLSCSDVAVEFGATTLLSDVSFTVAAGERWGIVGRNGAGKTT
                     LLRLIDGSL"
BASE COUNT          359 a          874 c         1048 g          389 t
ORIGIN      
        1 tgaaggcgcc gctcacggcc gacgactgag gatcgccggg ggcggtctcg ctcagcaggt
       61 cgggcagcgg cccggtgaag cgcgaatcga gccgcccggt gacgaggccc cggctgcgct
      121 gtagctccgc catgaactgc cgcaggttca cccgcaggtc cgccttcatc aggtagtcct
      181 cggagagccc ggtgtaggcc gccacatgtt tggcgaccgc cgccttgcgg tccgcgggca
      241 aggtcgcgcc cgcggccagc gcgtcggcat agtcccccat cgcgtagcgg cgcacttccg
      301 tgaggaacgc atcgaggttg gcgggcgccg ggatcacctt gtggtagacg gcggtggcgg
      361 cgtagctcgg cagatagagg acgtaggaca ggtcgtggcc gggcgagaac agcagggtct
      421 cgaagtccag caccgacgag atcagcacca tgccgttgaa cgtcatgccg tcgcgctgca
      481 gccggttcac gagaaccgcc gagcgggtcg tgccgtagct ctcgccgatg aggtacttgg
      541 gcgaggacca gcggccgttg cggctcacgt actgcgacac gaactgggcg agcgagcgcg
      601 catcctcgtc cacaccccag aagtccttcc cggtgccctt gccgacgggc ttggagaagc
      661 ccgtgccgac gggatcgatg aacaccaggt cggcgacgtc gatcaggctg ttggcgttgt
      721 ccaccatctg gtagggaggg ggcggcgtgg gcgccgcgtc ggtggtgacg atgcgccgcg
      781 ggccgaaggc gcccatgtgc agccacgccg aagccgagcc cggtccgccg ttgtacacga
      841 aggcgagggg ccgctgcgcg gggtcgcgcg cgtcgctgcg cgtgtaggcc gtgtagtaga
      901 ggctgccgat gggctcgtcc ttgtcgttcg tcaggagcat ggtggccgcg ctggcgcgat
      961 agggcacgag ctgaccgccg atgcggatgg tgtgctccgt gaccgaagac gtttccttcg
     1021 ggatggggcc ggccttcgcc gtcgtgtcgc ggtccgccgc ggcagccgcg ggctgggggg
     1081 gagcggcggg tcgttgggcg cgggccggag ccgcgagcgc gcatcccatc aggacgatcg
     1141 tgccgagacg catactgcct ccaacggtcg tgggtggggc gaagatgcgg cgcaacgcgc
     1201 gcacgcgcca gcaccgggcc gagggggcgc gcgatgcgcc atagtgaccg accccgccgc
     1261 gggctattct ccggcgatga cgcgcgtagc ggtgatcggg ggtggtgcgg cggggatgat
     1321 ggccgccgcc ttcgcggcgg ccggtggtgc agagacgctg ctgctcgagc acacgcccga
     1381 cggcggccgc aagatcctga tcagcggcgg cggccgctgc aatatcctcc ccgccgacct
     1441 cgacgagaca cggttcgtca ccgactcctc gccgcacacg ctgcgcaaca tcgtgcgctc
     1501 ctggccgttg cacgagcagc gcgcgttctt cgagcgggtg ctcgggcagc cgctggtcga
     1561 agagacggag tcggcgaagc tgttcccggc ctctcagcgg gcgcgcgacg tgcgcgacgg
     1621 actgctggcg cacgccgtgg cccggggcgc gcggtgggtg ccgtccacca ccgtctcggg
     1681 actggcgcgc gccggcgacg ggtggcgcat cgcctgtaag gacgaacgga cgctggatgc
     1741 ggaccgggtg atcgtggcga ccggcgggct ctcggtcccg aagaccggca gcgacgggcg
     1801 tggcctcgcg ctgctcgcag agctggggca cacgatccac gagccgtacg cggcgctcac
     1861 gccggtccag gccgaacccg cgccgttcgc cgcgctggcg ggcgtgtcgc tcacggtatc
     1921 gctcgtggcg cgcgatgcgg agcgcagcgc ccgggcgcgc gggggtttcc tgttcacgca
     1981 ccggggctac agcggaccgg cggtactgga cgtgtcgcac gtgctggtgc gctcgagcgg
     2041 cggcgcgcgt ctcatcgcgc agtggacggc gctcgacgcc gagggatggg agcgggtgct
     2101 gcgaggcgac ggcgcccgcg gggtggtggg cgtggtgcgg ggcgaaatgc ccgcgcggct
     2161 gggggatctg ctggtccgcc gggccggcat cgacccggag acgaagctat cgcagctgcg
     2221 gcgcgcggat cgcgtccggc tggtggaggg actcgcgcgg tgcgagctgc catggaccgg
     2281 tcacggcggc tatgcgaagg cggaggtgac gggcgggggc gtgagcctgg cggagatcga
     2341 cccccggacg ctggagagcc ggaaggcgcc cgggttgtat gtgtgcggcg aagtcctcga
     2401 cgcgttcggc ccgatcggcg gctacaactt cctgtgggcc tgggcgacgg gtcgcgcggc
     2461 ggggctggcc gcggcgcgcg ggaactgacc cgcctccccc atattccctc cgtgcctctc
     2521 ctgtcctgct ccgacgtcgc cgtcgaattc ggcgcgacga cgctcctcag tgacgtctcc
     2581 tttaccgtcg cggccggcga gcgctggggc atcgtgggcc ggaacggtgc cggcaagacg
     2641 accctgctcc ggttaatcga tgggagtctg
//
LOCUS       DBNGZW010000044         9912 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1640564, whole genome shotgun sequence.
ACCESSION   DBNGZW010000044 DBNGZW010000000
VERSION     DBNGZW010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9912)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1640564"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..1019)
                     /locus_tag="AC16JC_01065"
     CDS             complement(141..1019)
                     /locus_tag="AC16JC_01065"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase family protein"
                     /protein_id="HIR5163315.1"
                     /translation="MTGLTRRDLLAATAGAAVAALDSQSLPLAADPPVRAPADGRLKQ
                     SVCRWPFAGIPLPEFCRAARGIGLAAIDLLQPDEWAAARDAGLVCALGYPSKRRDFIA
                     TGFNDRANHALLVRELEETIPLAAKAGVPNVITMFGNRAGRSDDDGAAACIEGLRRVA
                     PVAEAHGVTVCVELLNSKVDHADYQGDHTAFGVRVVTGVGSPRVKLLYDIYHMQIMEG
                     DVIRTIRDHIAHIGHFHTGGVPGRHELDDTQELNYRAIAGAIADAGFAGYVAHEFEPT
                     REPLAALRDAVAVCRV"
     gene            complement(1089..1526)
                     /locus_tag="AC16JC_01070"
     CDS             complement(1089..1526)
                     /locus_tag="AC16JC_01070"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014853314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIR5163316.1"
                     /translation="MDDFLRASIAEAELGWSEGGIPIGSVLVHDGKILGRGHNRRVQQ
                     GSVVLHAEMDALERAGWQPASVYRASTLYTTLSPCAMCSGAILLYGIPRVVIGENRTF
                     LGEEKLLRSRGVTITIVQDARCRRLMADFIAAQPALWSEDIGR"
     gene            complement(1529..2275)
                     /locus_tag="AC16JC_01075"
     CDS             complement(1529..2275)
                     /locus_tag="AC16JC_01075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013284731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine fermentation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HIR5163317.1"
                     /translation="MSPLEKPRTRLLQAALNGSRRTEDHPTVPTTPAEVADAAAQCVK
                     AGARAIHVHVRDVRGRESVAPDDVARCVAALRAAIPGTPVGVSTGAWIIPEPELRHRT
                     IAAWTIQPDYASVNFHESGAEELATLLLSRGTGIEVGLPESAAAQRLVRSRLAPRCLR
                     VLIEPQAQELDAALRTVEEIEAVLDSAGVALARLLHGLDRTAWPLIGTAAARGYGTRI
                     GFEDILTLPDGSPAPTNAALVTAARPTFDW"
     gene            complement(2272..2934)
                     /locus_tag="AC16JC_01080"
     CDS             complement(2272..2934)
                     /locus_tag="AC16JC_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR5163318.1"
                     /translation="MISWECGAVLFDLDGVLVDSRRCIEAIWRLWAAGKGLDPEPFLR
                     IAHGRRTSETIRTVAPNLDVAAEVAVLDHLEEIETAGIHPAPGANQLVRSIPAAQWAI
                     VTSGSRQVASLRLGVVGLPIPEVFITAEAVRHGKPAPDGYLAAAARLGLEPAECVAVE
                     DSPPGIAAAREAGMRVIALLTTHQDRDLAQADIRLPALTHLGIAPRTRGRSAVALELS
                     PA"
     gene            complement(3026..3592)
                     /locus_tag="AC16JC_01085"
     CDS             complement(3026..3592)
                     /locus_tag="AC16JC_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR5163319.1"
                     /translation="MKRTLAITAAVLLCGAPSIARAQIGLKGGFSYGNVSNRGLLPGA
                     LHERNGFAVGLSLVSARSLLGFGIEGLYAQRGVVSNTAGESHKLDYIDVPAYLRVSLP
                     TPGIAPYAYAGPQVSFELHCTDACPSGRPKTTTAAVLGGGVRLGAQSAFSIEGRYVYG
                     LTDLKLNTVTSSDSYKTRSFLILAGIDF"
     gene            complement(3728..3904)
                     /locus_tag="AC16JC_01090"
     CDS             complement(3728..3904)
                     /locus_tag="AC16JC_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163320.1"
                     /translation="MSKSDEEQLAKLVKKNAAKAAQAKKTVQRRYDTTLPPETTDDDI
                     ERRDFFSEMKKRDF"
     gene            4169..4729
                     /locus_tag="AC16JC_01095"
     CDS             4169..4729
                     /locus_tag="AC16JC_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163321.1"
                     /translation="MTGLSQLVLPILLSAVFVFIVSSVIHMMLPWHKSDYPKVPNEDK
                     VMDALRPLGIPPGDYMLPRADSMQDMRSPAFIEKMKKGPVLMMTVLPSGQMGMGKNLV
                     QWFIYSVVVGIFGGYVAGRALPPGSSYLPVFRFVGVTAFCCYAVALWQFSIWYRRSWT
                     TTIQVTIDGLLYALLTAGTFGWLWPR"
     gene            complement(4737..5009)
                     /locus_tag="AC16JC_01100"
     CDS             complement(4737..5009)
                     /locus_tag="AC16JC_01100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019491887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 domain-containing protein"
                     /protein_id="HIR5163322.1"
                     /translation="MLVMIVEHFRGGDARPVYRRFRNRGRLAPEGLRYLASWVTEDFG
                     RCYQVMEYDDPALLRQWMAAWEDLVEFEVHPVRTSAAAAEFIAPRL"
     gene            complement(5062..5574)
                     /locus_tag="AC16JC_01105"
     CDS             complement(5062..5574)
                     /locus_tag="AC16JC_01105"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5163323.1"
                     /translation="MSFPDSFTTERLQAERLTADHLAELQRMHRDPAVMAHLGGVRDD
                     EGTAAYLARNLQHWTDYGFGLWILRELSGGDPVGRAVLRHLSVEGVDEVEVGYGFYQP
                     YWGRGLATEVTRACLTLGRRYLGLGSIVAVTSPANLASQHVLRKAGLACEREFLYEGT
                     ASLLFRTTDR"
     gene            complement(5576..5836)
                     /locus_tag="AC16JC_01110"
     CDS             complement(5576..5836)
                     /locus_tag="AC16JC_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163324.1"
                     /translation="MSCTIQKVDTAAGTIVAERLGRPDPNVGSRFDEIDVTVTRERGD
                     TTAIQITTASWTQFLQAAQRRRDPAASERAQVDGGRLLDVLH"
     gene            complement(5837..5986)
                     /locus_tag="AC16JC_01115"
     CDS             complement(5837..5986)
                     /locus_tag="AC16JC_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163325.1"
                     /translation="MPIRAPALAAIAWAAVPLHAQQGQQARLHSALVRHPPTRVLTWA
                     VDGLQ"
     gene            complement(6023..6439)
                     /locus_tag="AC16JC_01120"
     CDS             complement(6023..6439)
                     /locus_tag="AC16JC_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163326.1"
                     /translation="MSRAARLCAILLLAACRGSRSSGSAPLDTAAFTSVAPAGPLDST
                     LRIVITDQPRVYANGRLVSLVALDSLLGALKMIDGEVWFYRQEPDIHLAAQQNSLIDS
                     VFTAVRRHELPLTPSRMGDFGDLSDKRRRSPGAGGP"
     gene            complement(6450..6890)
                     /locus_tag="AC16JC_01125"
     CDS             complement(6450..6890)
                     /locus_tag="AC16JC_01125"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5163327.1"
                     /translation="MATRLVFSLFLLLGLRPAAVAGQGTTPEDSVRAVEMARGRALLH
                     ADTVALSRMLADEFVEISRLGAVRTRAVNIRDIASGELKLTSVRYDSLTVRIYGEVAL
                     LQGIADNAGTFRGFPFAGKIRYTRIFVRRDGRWQAVAMQQTSIQ"
     gene            complement(6907..7428)
                     /locus_tag="AC16JC_01130"
     CDS             complement(6907..7428)
                     /locus_tag="AC16JC_01130"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR5163328.1"
                     /translation="MGMVLRQVLAVAILPFTVTILVPAWIAGRYGIVPAPGASAAVLV
                     LQGGGLVVLAIGLVLFASSLRRFASEGEGTLAPWDPPRRLVVRGPYRFVRNPMISGVV
                     FVLAGEAMLLASRPHAIWAITFLGINLVYIPLLEEPMLRSRFGEAYREYCRHVPRIFP
                     RLRPWSPAGGGER"
     gene            complement(7473..8399)
                     /locus_tag="AC16JC_01135"
     CDS             complement(7473..8399)
                     /locus_tag="AC16JC_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007076180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR5163329.1"
                     /translation="MDILAAIGNTSLVRLRKVVPTDSADILVKLEWENPSGSVKDRMA
                     WAVIERAEADGRLRPGDTVVEYTGGSTGTSIALVCAAKGYRLHIVTSDAFSQEKRDHM
                     AALGAELTLVPSQGGRTTRQLILDMIEAARELSRQPHTYWTDQLNNRDSIAGYEPLGE
                     EIWSQTKGAVTAFVHSVGTAASLRGTATVLKRHQPAVEILAVEPAESSVLRGGQPGPH
                     KIEGIGIGYIPPLWDPALVNDILPVSTEDAKAMARRLAREEGLFAGTSSGANVIAAIQ
                     VAQRLGPGATVVTLLADSGLKYLSTDVYRRAP"
     gene            complement(8505..8849)
                     /locus_tag="AC16JC_01140"
     CDS             complement(8505..8849)
                     /locus_tag="AC16JC_01140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010959695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HIR5163330.1"
                     /translation="MRIRTKRIYESPVPADGRRILVDRLWPRGLTKTAARIDYWARAI
                     APSTQLRRWYGHDPAKWREFRRRYFAELDDNPAGLTDLRRHLSKGTVTLLYSSKEERL
                     NNATALREYLEA"
     gene            complement(8867..9298)
                     /locus_tag="AC16JC_01145"
     CDS             complement(8867..9298)
                     /locus_tag="AC16JC_01145"
                     /inference="COORDINATES: protein motif:HMM:NF020397.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HIR5163331.1"
                     /translation="MTVLEFLDQLPPARRHEVDRLRTLIRRHLPAGYEEVVSKRMLVY
                     QVPLARYPDTYNKQPLWYIALASEKSYLSLHLMSIYGDATQAERLKTSFKAAGKKLDM
                     GKACIHFKTADDLPLDAIGQVVASMPPDRWVAVAQAARRNH"
     gene            complement(9295..9534)
                     /locus_tag="AC16JC_01150"
     CDS             complement(9295..9534)
                     /locus_tag="AC16JC_01150"
                     /inference="COORDINATES: protein motif:HMM:NF018595.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HIR5163332.1"
                     /translation="MAASKRLQVYETPQITVTFDPNVCVHSGVCVRGLPAVFDVKRAR
                     WVRPELASAEAVAAQVARCPSGALQCRVKGGEAPA"
     gene            complement(9566..>9912)
                     /locus_tag="AC16JC_01155"
     CDS             complement(9566..>9912)
                     /locus_tag="AC16JC_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR5163333.1"
                     /translation="RSKRVEVRSIAPDANPYLAIYTIFKTGLEGPMEEGDESKRSRTR
                     FLSDNIYDAIRLYKASPFLKQMLGEEVHGKFADLKQAQADRCPKALGAEVKAAEIQFH
                     HEVTNQYLWSKF"
BASE COUNT         1543 a         3292 c         3454 g         1623 t
ORIGIN      
        1 gtcggcgaag cgtaccagaa agttctggtc gcgctcgctc ggcagcgggc ggacgttccc
       61 cgccccgccg aaacgacgcg acaggatctc ttccgcttcc gctggagaaa acgccggctg
      121 ccgaaacgtg atgccgaggg tcacacgcgg cagacggcga cggcgtcgcg cagcgcggcg
      181 agcggctcgc gcgtcggttc gaactcgtgc gccacgtatc cggcgaatcc ggcgtccgcg
      241 atcgcgccgg cgatcgcccg gtagttcagc tcctgcgtgt cgtccagctc gtggcggccg
      301 gggacgccgc cggtgtgaaa gtggccgatg tgggcgatgt ggtcgcgaat cgtccggatg
      361 acgtcccctt ccatgatctg catgtgatag atgtcgtaca ggagcttgac ccgcggcgag
      421 cccaccccgg tgacgacacg gacgccgaac gccgtgtgat caccctgata atccgcatgg
      481 tccaccttgc tgttcagcag ttcgacgcac acggtgaccc cgtgggcctc cgccaccggc
      541 gcgacgcggc gcagcccttc gatgcaggcg gcggcgccgt cgtcatcgct gcggccggcg
      601 cggttgccga acatcgtgat cacgttgggg acaccggcct tggccgcgag cggaatcgtt
      661 tcctccagct cgcgaacgag cagcgcgtgg ttggcgcggt cgttgaatcc cgtggcgatg
      721 aagtcccggc gtttggacgg atacccgagc gcgcacacga gccccgcgtc gcgggcggcc
      781 gcccactcgt cgggctggag cagatcgatg gccgcgagcc cgatgccccg ggccgcgcgg
      841 cagaattccg gcagcggaat gccggcaaag ggccagcggc agaccgattg tttcagcctc
      901 ccgtccgccg gagcccggac cggaggatcg gcggccaggg ggagcgattg actgtcgagg
      961 gcggcgacgg ccgctcccgc ggtggcggcc agcaggtctc gccgggtcag tcccgtcatc
     1021 gtgatctcca aggtgcggcg gtagtcgaaa cttgcaccgc cgctccgctt cccgaaagga
     1081 cgcgtgtgtc agcgcccgat gtcttcggac cagagcgcgg gctgggcggc gatgaagtcg
     1141 gccatgagac ggcgacagcg cgcatcctgg acgatcgtga tggtcacccc gcggctgcgc
     1201 agcagcttct cctcgcccaa aaacgtgcgg ttctcgccga tcaccacgcg tgggatgccg
     1261 tacagcagga tcgcgccgct gcacatcgcg cagggcgaga gcgtggtgta gagcgtgctc
     1321 gcgcggtaga cggacgccgg ctgccacccc gcgcgctcga gcgcgtccat ctcggcgtgc
     1381 aggacgacgc tgccctgctg gacgcgacgg ttgtgtccgc gacccagaat ctttccgtcg
     1441 tgcaccagca ccgagccgat gggaatgccg ccttcgctcc agcccagctc cgcctcggcg
     1501 atcgaggcac gcaggaagtc gtccatggtc accagtcgaa cgtgggacgg gccgccgtga
     1561 ccagcgcggc gttggtggga gcgggcgacc cgtcgggcag tgtcaggatg tcttcgaagc
     1621 cgatgcgggt gccatagccg cgcgccgccg ccgtgccgat cagcggccag gccgtgcggt
     1681 cgaggccatg cagcaagcgc gccagcgcga ccccggccga gtcgagcacc gcttcgattt
     1741 cctccactgt ccggagcgcc gcgtcgagct cctgggcttg cggctcgatc aggacgcgga
     1801 gacagcgtgg agcgaggcgg ctgcgcacca gacgctgtgc ggccgccgac tccggaagtc
     1861 ccacttcgat gcccgttccg cgggagagga gcagcgtggc cagctcttcg gcgccggact
     1921 cgtgaaagtt gacggaggcg tagtcgggct ggatggtcca ggccgcgatg gtgcgatggc
     1981 gcagttcggg ttcggggatg atccaggctc cggtgctcac ccccaccgga gttccgggga
     2041 tcgcggcccg caacgcagcg acgcaacgcg cgacgtcgtc gggtgcgacg ctctcgcgcc
     2101 cccggacatc gcggacatga acgtggatcg cacgagcacc cgccttcacg cattgcgcgg
     2161 cggcgtccgc cacctcggcc ggcgtcgtgg gcaccgtagg atggtcttcc gtccgtcggc
     2221 tcccgtttaa tgcggcctgg aggaggcggg tgcgtggctt ctcgagcggg ctcatgcggg
     2281 agacagctcg agggcgaccg cggaacgccc gcgcgtccgg ggcgcaatgc cgagatgggt
     2341 gagcgcgggc agccggatat ccgcctgggc caggtcccgg tcctggtgcg tggtgaggag
     2401 agcgatcacg cgcatcccgg cttcccgtgc cgccgcgatc cccggcggag agtcctcgac
     2461 cgccacgcac tcggcgggct ccagtcccag ccgggcggcg gcggcgaggt agccgtcggg
     2521 ggccggcttc ccgtgtcgca cggcctccgc cgtgatgaag acctccggga tcggcaatcc
     2581 gaccacgccg agccggagcg aggcgacttg gcggctgccg gaggtcacga tcgcccactg
     2641 cgcggccggg atcgacctga cgagctggtt cgcgccgggc gccgggtgaa tgccggcggt
     2701 ttcgatctcc tccaggtgat cgagcaccgc gacttcggcg gcgacatcga ggttgggagc
     2761 caccgtccgg atcgtctccg aagtccgccg tccgtgcgcg atgcgcagga agggttccgg
     2821 gtcgagcccc ttccccgccg cccagaggcg ccagatcgcc tcgatgcagc gccgcgagtc
     2881 gaccagcacg ccgtcgagat cgaacaggac ggccccacat tcccaggaga tcaccagggc
     2941 acccgtgtca agctgtccgg cgaaggtaag gcccgggacc ggccccttga agggccggcc
     3001 cggggtgtgt cgtaggggcc ggtcgctaga agtcgatacc ggccaggatg aggaaggacc
     3061 gggtcttgta gctgtccgag ctggtgaccg tgttgagctt gaggtcggtg agtccgtaca
     3121 catagcgccc ctcgatcgag aacgcgctct gggcgccgag gcgcacgcct ccgcccagga
     3181 cggcggccgt ggtcgtcttg gggcgtccac tcggacacgc gtcggtgcag tgcagctcga
     3241 aggagacctg cggaccggcg tacgcgtacg gagcgatccc cggtgtcggg agcgagacgc
     3301 gcaggtacgc aggcacatcg atgtagtcga gcttgtggga ttcaccggcc gtattgctga
     3361 ctacgccgcg ctgggcgtac agcccttcga taccgaatcc caacaggctg cgtgcgctca
     3421 cgagcgagag tccgaccgcg aacccgtttc gctcgtgcaa cgcgccgggc agcaggcccc
     3481 ggttggagac gttgccgtag gagaagccgc ccttgagtcc gatctgggcg cgtgcgatgc
     3541 tgggagcccc gcagagcagg acggcggcgg taatagcgag tgtgcgtttc atcgtcggtt
     3601 cctccgaacg aggagacgtt cagagctacc ccgacgagcg ggcgtgggtt gccgtggaac
     3661 aggtgcagct tgaaactgcc ggtcgcacat cagcacgcgg aggtgacccg cgtcaccggc
     3721 gacggctcta gaagtcccgc ttcttcatct ccgagaagaa atcgcgccgt tcgatgtcgt
     3781 cgtccgtcgt ctcaggcggc agcgtcgtat cgtagcggcg ctgcaccgtc ttcttcgcct
     3841 gcgccgcctt ggccgcgttc ttcttcacca gtttggccag ctgttcctcg tcgctcttgg
     3901 acaccccgca gctcctgtct ggaggattcg ccgggaaaga tacaaacatc gcctccagcc
     3961 tggtactcgg cccgggagag cgcacgggaa ggggcgttca agggacgtgc tcgcgccggc
     4021 cgatgcgcag tgtgaccgcg gcccgctgat ctcccgcagg aacgtgcgcc cgctcgcttg
     4081 acgccccggc ctcgcgcgcg gcagctttga tggttggcac ggggacccga gtgccggccg
     4141 atcgggccat ctctttccgg agaatcgtat gacaggactc agtcagctgg tgctgccgat
     4201 tctgctgtcg gcggtgttcg tgttcatcgt gagctccgtg atccacatga tgctgccgtg
     4261 gcacaagagc gattatccca aggtgccgaa cgaagacaag gtcatggatg ctctgcgacc
     4321 cctgggcatc cctccgggcg actacatgct tccccgcgcc gacagcatgc aggacatgcg
     4381 ctctccggcg tttatcgaga agatgaagaa ggggccggtc ctgatgatga ccgtgctgcc
     4441 gagcggtcag atggggatgg ggaagaatct ggtgcagtgg ttcatctatt cggtcgtcgt
     4501 cggcatcttc gggggctatg tggccgggcg cgccctgccg ccgggctctt cgtacctgcc
     4561 ggtgttccgt ttcgtcggcg tcacggcgtt ctgctgctac gccgtggccc tgtggcagtt
     4621 ctcgatctgg taccgtcgct cctggaccac tacgatccag gtgacgatag acgggctgct
     4681 ctacgccctc ctcaccgcag gcacgttcgg ttggctgtgg ccgcgctagc ggagggctag
     4741 agtcgagggg cgatgaattc ggctgctgcg gcggaggtcc gcaccgggtg gacttcgaac
     4801 tcgaccaggt cctcccacgc ggccatccac tgccgcagca gcgcggggtc gtcgtactcc
     4861 atgacctggt agcagcggcc gaagtcctcg gtgacccagc tggcgaggta gcggagcccc
     4921 tcgggtgcga ggcggccccg gttgcgaaag cggcgataca ccgggcgcgc gtccccgccc
     4981 cggaagtgct cgacgatcat gaccagcatt ggctactccc gggtcccgtg acgtcgtagt
     5041 ggaggactgt aagcggggcg atcatcggtc ggtggttcga aacagcagtg aggccgttcc
     5101 ctcgtacagg aattcacgct cgcaggcgag tccggccttt cgcagcacgt gttgcgacgc
     5161 caggttggcc gggctggtca cggcgacgat cgagccgagg cccaggtacc ggcgtcccag
     5221 cgtcaggcag gctctggtca cttcggtcgc gagtccccga ccccagtacg gctgatagaa
     5281 cccatagccg acttcgactt cgtcgacacc ctcgaccgag aggtgccgca gcaccgcacg
     5341 cccgaccggg tcgcccccgc tcagctcccg gaggatccac aagccgaagc catagtcggt
     5401 ccaatgctgc agattgcggg cgaggtacgc ggccgtcccc tcgtcgtcgc gcacaccgcc
     5461 gagatgggcc atcacggcgg gatcgcgatg catgcgttgc agctctgcca ggtgatcggc
     5521 ggtcagacgc tcggcctgga ggcgttcggt cgtgaaggaa tcgggaaagg acatgttagt
     5581 ggagcacgtc gagcagccgg cccccatcca cttgcgcccg ttccgaggct gcgggatcgc
     5641 ggcgccgttg cgccgcctgg aggaattgcg tccaggaggc cgtggtgatc tggatcgcgg
     5701 tcgtgtcgcc gcgctctcga gtgaccgtga cgtcgatctc gtcgaaccgg ctgcctacgt
     5761 tcgggtccgg acgtcccagc cgctccgcga cgatggtccc ggcggcggtg tcgaccttct
     5821 gaatcgtgca gctcatttac tgcagtccat cgactgccca ggtcagtacc ctggtcggcg
     5881 gatgtcgcac cagggcgctg tggagccgcg cctgctgtcc ctgctgcgcg tgcaacggca
     5941 cggcggccca ggcgatcgcg gccagggcgg gtgcccggat tggcatgtca acctcctgaa
     6001 tgccaccccg ccggcgtgca cattacggcc cccccgcccc gggagagcgg cgacgcttgt
     6061 cggacaggtc cccgaaatcg cccatgcggg aaggcgtgag cgggagctca tgccggcgca
     6121 ccgcggtgaa gacggagtca atgagcgagt tctgttgcgc cgccaggtgg atatcgggct
     6181 cctggcggta gaaccagact tccccgtcga tcatcttgag ggccccgagc aacgaatcca
     6241 acgcgacgag agagacgagc cgcccgttgg catagacgcg cggctgatcg gtgatgacga
     6301 tgcggagggt ggaatccaac ggcccggcgg gcgctactga cgtaaacgcg gcggtatcca
     6361 gcggtgcgct gccgctcgat cgactcccgc ggcatgccgc gaggagcaga atggcgcaga
     6421 ggcgtgcggc ccgggacacg tggcgatcgc tactggatgc tcgtctgttg catcgccacc
     6481 gcctgccagc gtccgtcgcg gcggacgaag atccgggtgt acctgatctt cccggcgaac
     6541 gggaagcccc ggaatgtgcc ggcgttgtcc gcgatgccct ggagcaacgc gacctctcca
     6601 tagatccgca cggtgagact gtcgtaccgc acggaggtca gcttcagctc cccgcttgcg
     6661 atatcgcgga tgttgacggc cctcgtgcgc acggccccga gtcgcgagat ctcgacgaac
     6721 tcgtcggcca gcatccggga cagcgcgacc gtgtccgcgt gcagcagcgc ccgcccgcgg
     6781 gccatctcga cggcgcgcac ggagtcctcc ggcgtcgtgc cctgcccggc caccgctgcg
     6841 gggcgcaacc cgaggagcaa gaacaggctg aaaacgagtc tcgtggccat gaggcgtttc
     6901 tcgaacttaa cgttctccgc cgcccgcggg cgaccacgga cggagacgcg gaaagattct
     6961 cggcacatgc cggcagtact cgcggtacgc ctcgccgaac cggctccgga gcatcggctc
     7021 ctcgagcagg gggatgtaga ccagattgat gcccagaaac gtgatcgccc agatcgcgtg
     7081 cggtcgcgag gcgagcagca tcgcctcacc ggcgaggacg aagacgacgc cggaaatcat
     7141 gggattccgg acgaaccggt agggtccgcg cacgaccagc cgccgcggcg ggtcccacgg
     7201 cgccagggtc ccctcgccct cggaggcgaa gcggcgcagg gaggaggcga agagaaccag
     7261 gccgatcgcc agcacgacga gtcccccgcc ctgcagcacg agtacggcgg ccgacgcgcc
     7321 gggtgccggg acgatgccat accgtccggc gatccaagcg gggacgagga tcgtcaccgt
     7381 gaacgggagg atcgcgaccg ccaggacctg ccggagcacc attcccatgg ttgcgacaaa
     7441 ctgctcctcg caacggccgc aagcaagaga gcctagggtg ctcgccggta cacgtcggtg
     7501 ctcaggtact tcagcccgga gtcggccagc agggtgacga ccgtggcacc cggccccagg
     7561 cgctgggcca cctgaatcgc cgcgatcacg ttcgctccgg aggacgtgcc cgcgaacagg
     7621 ccttcctccc gcgcgaggcg ccgggccatg gctttcgcgt cttcggtcga aaccggcagg
     7681 atatcattca cgagcgccgg atcccagagc ggggggatgt agccgatgcc gattccttcg
     7741 atcttgtgcg ggccgggctg tccgccccgc agcacggagg attcggcggg ctcgacggcc
     7801 aggatctcga cggccggctg gtgtcgcttg agcaccgtcg cggttccccg cagcgaggcg
     7861 gccgttccga cggaatgcac gaaggcggtg accgctccct tcgtttgact ccagatctcc
     7921 tcgcccaacg gttcgtagcc cgcgatgctg tcccggttgt tgagctggtc ggtccagtag
     7981 gtgtggggct gccggctcaa ctcgcgggcg gcttcgatca tgtccaggat cagctgcctg
     8041 gtggtgcggc ctccctggct cgggaccagc gtcagctccg cccccagcgc cgccatgtgg
     8101 tcccgcttct cctggctgaa cgcatcggac gtgacgatgt ggaggcggta acctttggcg
     8161 gcgcagacca gcgcgatcga agtccccgtg ctgccgcccg tgtactcgac gaccgtgtca
     8221 ccgggccgca gccggccgtc cgcctccgcg cgctcgatca cggcccacgc catccggtcc
     8281 ttgacgctgc cgctggggtt ctcccactcg agcttcacga ggatgtctgc gctgtcggtg
     8341 ggaacgactt tgcgcaggcg aaccagcgag gtgttgccaa tggcggcgag aatgtccacg
     8401 gtcggctccc ggctgggcgg aatgctccgg ttccggcggc agaccgttcg gaaccccgct
     8461 gcgctgaacg cgcagccggg gagcgaggtt cccgacttcg agcgctacgc ctccagatac
     8521 tcccgcagcg ctgtggcgtt gttcagtcgc tcctccttgg agctgtagag gagcgtaacc
     8581 gtgcccttgc tcaggtgccg gcgcaggtcc gtcagtccgg cggggttgtc gtcgagctcg
     8641 gcgaagtagc gtcgccgaaa ctccctccac ttggccgggt cgtggccgta ccagcgacgg
     8701 agttgcgtgg agggagcgat cgccctggcc cagtagtcga tccgcgcggc cgtcttggtg
     8761 aggccgcggg gccacagacg gtcgaccagg atgcgccggc cgtccgcggg gacgggtgac
     8821 tcataaatgc gcttggtgcg gattcgcatg gggctgacgc gaagggtcag tggttgcgac
     8881 gcgctgcctg tgcgacggcg acccagcgat ccgggggcat actggcgacg acctgcccga
     8941 tcgcgtccag tggcaggtca tccgccgtct tgaagtggat acaggccttg cccatgtcga
     9001 gcttcttccc ggcggccttg aagctggtct tgagccgctc ggcctgagtg gcgtccccat
     9061 agatgctcat caggtgcagg gacaggtagg acttctccga agcgagcgcg atgtaccaca
     9121 gcggctgctt gttgtaggtg tccgggtatc gcgcgagcgg cacctgatag accagcatcc
     9181 gcttgctgac gacctcctcg tacccggcag gcaggtgccg gcggatgagc gtgcgcaggc
     9241 ggtcgacctc gtggcggcgt gcgggcggca gctggtcgag aaactcgagg acggtcacgc
     9301 cggagcttcc ccgcccttca cgcggcactg cagcgctccg gaggggcaac gggctacctg
     9361 ggccgcgacc gcttccgccg acgccagctc gggccgcacc caacgggccc gcttgacgtc
     9421 gaatacggcc ggtaatccac gcacgcagac gcccgagtgc acgcacacgt tcggatcgaa
     9481 cgtgacggtg atctgtggag tctcgtagac ctgcaggcgt ttcgacgcgg ccacgtgagg
     9541 tcctcccccc ggagcggccc ctccgctaga acttggacca gaggtactgg ttcgtgactt
     9601 cgtggtggaa ctggatctcg gcggccttga cctccgcgcc gagcgccttg gggcaccggt
     9661 ccgcctgcgc ctgcttcaag tcggcgaact tgccgtggac ttcctcgccg agcatctgct
     9721 tgaggaaggg actggccttg tagaggcgaa tggcgtcgta gatgttgtcc gacaagaacc
     9781 gggtgcggct ccgcttgctc tcgtcgccct cttccatcgg tccctccagg ccggtcttga
     9841 agatcgtgta gatcgccagg tacgggttgg catcgggcgc gatcgaccgg acctccaccc
     9901 gcttggaacg tt
//
LOCUS       DBNGZW010000045        11452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1231641, whole genome shotgun sequence.
ACCESSION   DBNGZW010000045 DBNGZW010000000
VERSION     DBNGZW010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 11452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11452
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1231641"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..575
                     /locus_tag="AC16JC_01160"
     CDS             39..575
                     /locus_tag="AC16JC_01160"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5163334.1"
                     /translation="MKLDARTVALVLIGAPGLLAAQGGASPVADAFRTYETRSARKLV
                     AAIEAMPADKLGYKPTPAQMSVAAIAVHLAEGNDYFCSTISGTPAPKRDSVTASSSKE
                     ALVARLRETFDFCQTTLAKLDDSRLGEELPLFGGNKYTRASVILITTGDWEDHYSQLA
                     NYLRLNGLLPPTARPQGQ"
     gene            complement(614..1078)
                     /locus_tag="AC16JC_01165"
     CDS             complement(614..1078)
                     /locus_tag="AC16JC_01165"
                     /inference="COORDINATES: protein motif:HMM:NF042842.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6790 family protein"
                     /protein_id="HIR5163335.1"
                     /translation="MHLHFVYLLALLLLAATVHAAVLPERTTSRVAEIGVLYLLVGYC
                     GVPMLAVSLWVLIGPEHAAAGFGFTASGPLLAFFGWAYLGMSLLSLLALRYRGAFLVA
                     PVVVWAVYLGGAMAVHLRESHGRALEVFASHGLVAALLMVGLLASGAWKARA"
     gene            1208..1777
                     /locus_tag="AC16JC_01170"
     CDS             1208..1777
                     /locus_tag="AC16JC_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5163336.1"
                     /translation="MTRNATVHARPVPDRRVRRTRHRLKEALLRLIEERSYDRITVAD
                     ITHHADVGRSTFYSHFDSKEELLFDGFEAGLRALAAPGPSGGAGFCFSLPLLRHIGTQ
                     PRFALATLGRSANSAIQQTTTRILSDIVRLELERSSASSGAAREAEVHGIVGAFRGLV
                     TWWLTSGSRMSPEALDQVFQRLVSGIGRA"
     gene            1774..3423
                     /locus_tag="AC16JC_01175"
     CDS             1774..3423
                     /locus_tag="AC16JC_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011557853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR5163337.1"
                     /translation="MTRDTMLDLGRDAVRRHAWREAFTSLSAADQQSPLEPTDLEALA
                     SAAQLLGRDGESADLWERAHHGFLGRGDVERAARCAFWLAFGLFEHGETARAGGWLAR
                     ARRLLDDGRRDCVELGYLLLPLARQQVAAGDIAAAYDSFARAAEIGDRFGDRDLTTLG
                     RHGVGRALIRRGDIAVGVALLDEVMVGVTGGEVGPVVAGAVYCSVISACQDMFDLRRA
                     QEWTAALSRWCDSQPELVPYRGECQVRRSEILQIHGAWPDAVDEARQACERLSQPPEH
                     PSLGAAHYQLAELHRLRGEFAEAEAAYRAASRWRKPQPGLALLRLAQGQLEAAHAAIR
                     GALDEASGDAARARILAAFVEIALAASDLAGAEAAAHELAGIAGRLDARYLAALSDQT
                     SGAVLLAQRRPREALAALRRAWTAWGDLEAPYEAARVRVLVAQACRSQGDRDTAELEL
                     DAARAAFEQLGAAPALARLEALAGVEAPPAVSPLSGRELQVLRLVASGKTNRAIAEAL
                     GISEKTVARHVSNIFTKLGLSSRAAATAYAYQHDLVVARST"
     gene            complement(3458..6145)
                     /locus_tag="AC16JC_01180"
     CDS             complement(3458..6145)
                     /locus_tag="AC16JC_01180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR5163338.1"
                     /translation="MLAELLSDLRYRLRALLRRDALEQDLDQELRFHIEQQAEQYVRA
                     GDPPAEALRKARAAFGGVERIKDDARDARGLMLLDAVTHDLRYAARGLRLSPGFTLAV
                     VATLALGIGANAAVFDVLDLLLFRPPAYLHEPRSVHRVYLASTFRGQPFTSHTFEFRR
                     YLDLTSATRSFAQVAAFATFPAALGTGPDITEASVGTASAGFFAFFDARPVLGRFFDR
                     VEDSLPAGAPVAVLDYGYWQSHYGGRRDILGMPIQIGPATLTIIGVAPSGFIGVPDGR
                     RPVAWVPITAYAWMRRHDYYLNYNWGWLQIVVRTKPGVRLDAATQDLTAAYRRSWLAE
                     RAMDPSLAALDIAQPHAILGPTQLERGPEAGPGTRIGLWIGGVALVVLLVAAANVANL
                     LLARAFRRRREIAVRLALGVSRGRLLGHLLTESLLLAVLGGAAGLVLAWAGGAALTRI
                     FTTDLAPAPLTDTRTMLFCALLTLVVGVLTGLAPALPAWRGDLVSGLKAGAREGSYQR
                     SRLRALLVVVQGALSVALLVGAGLFVRSFSNVRALHLGYDVDPVLYVERNMRGVNLPE
                     DQQAALNHRLVLAAAELPGVVSATEAVSVPFYNFESRGLFVPGIDSVSRLGRFQLQAG
                     SPDFFRTLGTRILAGRGITAEDRRGAPPVIVVSEAMAQRLWPGRKALGQCVRVGADTA
                     PCRTVVGIVEDIKQRSLTEIGAATYYLPADQLDEGAYGLFVRVGGRAVDASATIRRRL
                     QQEMPGMSYVTIVPLTQILDGEQRPWRAGATMFLVLGGLALIVAAIGLYSVIAYNVAQ
                     RTQELGVRIALGARLRDVLWLVLGDGLRLALGGIAAGAVLALAGGRWIGTLLFAVSPR
                     DPTVFGTVTLVLALTALLASLIPALRAARVDPNLALRLD"
     gene            complement(6147..6479)
                     /locus_tag="AC16JC_01185"
     CDS             complement(6147..6479)
                     /locus_tag="AC16JC_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020713226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR5163339.1"
                     /translation="MTEDRLDLPQGTLDLLILKALSLGPQHGWAISERLHQVSRATLQ
                     VPQGSLYPALHRLERRGWIAATWGTSDNNRRAKYYALTSAGRKQLDAEARAWSKLTHA
                     VGLVMEMG"
     gene            6657..7787
                     /locus_tag="AC16JC_01190"
     CDS             6657..7787
                     /locus_tag="AC16JC_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin-containing monooxygenase"
                     /protein_id="HIR5163340.1"
                     /translation="MSSRHEIERIQTVVIGGGQAGLSVGYQLARRGLPFLILDANARV
                     GDSWRRRWDSLRLFTPARYDGLAGFPFPAKRDAFITKDEMADYLEAYAARFRLPVRNG
                     ARVDGLSRRGDRFLVTAGERRYEADQVVVAMANYQVPRVPEFASALDRGILQLHSREY
                     RNPSQLARGGVLVVGVGNSGADIALEAARTHATWLAGKEAGHVPFRIERWLARHFLVR
                     VVRFVGHRVLTRGTPIGRKLTPTLLHRAAPLVRVKPADLTAAGIVRVPRVVGVRDGRP
                     LLADDRTLDVATVIWCTGFHPGFSWIQLPVLGDDGLPRHERGVVVEQPGLYFVGLHFL
                     YAMSSDTVNGVGRDAEWIARAIAVRVRDARSVPAANPAAVAA"
     gene            complement(7808..8515)
                     /locus_tag="AC16JC_01195"
     CDS             complement(7808..8515)
                     /locus_tag="AC16JC_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HIR5163341.1"
                     /translation="MGFSSETLRFFRGLARRNEKPWFEAHRDDYEQHVRGPMRELVEE
                     MDVRLARFAPEIVGDPRRSIFRINRDIRFSRDKSPYKTHIGAWFYHRDATRKVGQDGE
                     GGSAGFYVQVAPGDSFTGGGIWMPPRAALARIRDAVAENPRAFFRIADHPALRRRLGG
                     LTREDALKRTPRGYPPDHPAATWLRLQSFTAGRTLSDTDALSPRLVTLLERQFLLMLP
                     LVRWLNGALGLRPASQR"
     gene            complement(8546..8977)
                     /locus_tag="AC16JC_01200"
     CDS             complement(8546..8977)
                     /locus_tag="AC16JC_01200"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR5163342.1"
                     /translation="MTDRAAIADRLHSLAIHLLRRVARSDQQSGVTGPRLSVLSVLVL
                     GGGPRPVGELAAAEHVRPPTMTRLVQAMEAEGLVTRTPAPEDARIVRIRATRAGERVL
                     AKARARRLSALRALLTDLAAADLAALQRAVAILESVVSKKP"
     gene            9100..9759
                     /locus_tag="AC16JC_01205"
     CDS             9100..9759
                     /locus_tag="AC16JC_01205"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR5163343.1"
                     /translation="MPTVVDLSVAIRDRSVPRVAAILREAPALAAARPAGNPSPLLLA
                     VYVNAPDILALLRPHVMLDACEAAAAGDVERLRTLLAGDAAQVYARSGDGWTPLHLAA
                     FFGRREAVELLLASGAPISAVSGGQERNQPLHAALAGACDRVIVRALVMAGADVNAVG
                     AMGVTPLHEAASRGNAELVRFLRAAGANVMATLDDGKTPAQLAAARGHAALAEELRAL
                     V"
     gene            complement(9776..10471)
                     /locus_tag="AC16JC_01210"
     CDS             complement(9776..10471)
                     /locus_tag="AC16JC_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011287834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR5163344.1"
                     /translation="MPPRHVEVHRSGRIGWLRAAVLGANDGIVSTASLILGVAAAEAS
                     PHQVLVAGVAGLVAGAMSMAAGEYVSVSSQADTERADLAREQHELASQPAHERAELIG
                     IYEARGLTRALAEQVTDQLMAHDALGAHAREELGIVDMHRARPIQAALASAATFAVGA
                     FLPLLAALLAPSGTMLPVVALSSLAFLAGLGAVSARTGGAPVATATLRVTFWGALAMA
                     LTAGAGALFGAAG"
     gene            complement(10494..10751)
                     /locus_tag="AC16JC_01215"
     CDS             complement(10494..10751)
                     /locus_tag="AC16JC_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163345.1"
                     /translation="MKATTTKRRARRPVRRRGISRIDQDSTRTHGWYVRAGFHRRRDG
                     MYVPRHSRFFADLSWGGKRRALQAAKEYLAKLTRPARRAAA"
     gene            complement(10842..>11452)
                     /locus_tag="AC16JC_01220"
     CDS             complement(10842..>11452)
                     /locus_tag="AC16JC_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR5163346.1"
                     /translation="RLTQGPVPAIYQPLNQVSLTFFLSHMTFLARTDAAPGEYARAVR
                     ATLRDIDPDLPPQALASLQDFIAGIRATPLFQTRILVVFSLLALSLAAVGIYGLLAYS
                     VMERTHEIGIRLALGAPIGRVMRLVLTRTLRLAGLGVVAGTTGALIATRALSKLLYET
                     SPTDPATFAAVTALLIAVALVASLVPARRASRVDPVRALRAE"
BASE COUNT         1705 a         3939 c         4233 g         1575 t
ORIGIN      
        1 aaggcgcaga atcaacccac cccgaaatgg agagcgtcat gaagctggat gcacgcaccg
       61 tcgccctggt cctgatcggt gcccccggat tgctggccgc ccagggcgga gcgagcccgg
      121 tggcggacgc gtttcgcacc tacgagaccc gctcggcccg gaagctcgtc gccgccatcg
      181 aggcgatgcc ggccgacaag ctcggctaca agcccacacc ggcgcagatg agcgtcgccg
      241 ccatcgcggt gcacctcgcg gagggcaacg actacttctg ctctaccatc agcgggacgc
      301 cggctccgaa gcgggactcg gtcaccgcct cgtcgtccaa ggaggcgctc gtggcacggc
      361 tgcgcgagac gttcgacttc tgccagacga cactcgccaa gctcgacgac tcgcggctcg
      421 gcgaggagct gccgttgttc ggcggcaaca agtacacgcg cgcgtccgtc atcctgatca
      481 ccaccggaga ctgggaagac cactacagcc agctcgcgaa ctacctgcgg ctcaacggtc
      541 tgctcccacc cacggcgcgg ccccaggggc agtaacggcg gcggtccggg cggcgcacgc
      601 gccgcccggc ccctcacgca cgggccttcc aggccccgct cgcgagcagc cctaccatca
      661 gcagcgccgc cacgagtccg tggcttgcga aaacctcgag cgcgcggccg tgcgattctc
      721 gcaggtgcac cgccatcgcc ccgccgaggt acaccgccca caccaccacc ggggcgacga
      781 gaaaggcgcc ccgatatcgc agcgccagga gcgacagcaa cgacattccc aggtaggccc
      841 agccgaagaa cgccagcagc ggtccgctcg cggtgaagcc gaatcccgcc gccgcgtgct
      901 ccggaccgat gagcacccag agactcaccg ccagcatcgg caccccgcag tacccgacga
      961 gcagatagag caccccgatc tcggcgacac gactggtcgt ccgctccggg aggaccgcgg
     1021 cgtggaccgt cgcggcgagc agcaggagcg ccaacaggta gacgaaatgc aggtgcacga
     1081 ggccctcgtc gttgctggac tgttgttcgg aaatggacta gtgtccgata cacgctggac
     1141 ggcgggccgc ccaaggtcaa ccagcaaggc ggcgccgggt gtcccgaatt ggacgcggag
     1201 cactcacatg accaggaacg caaccgtcca tgcgcggccg gtgcccgacc ggcgggtgcg
     1261 gcgcacgcgc catcggctga aggaagccct gctgcggctg atcgaggagc ggagctacga
     1321 tcgcatcacc gtcgcggaca tcacccacca tgccgacgtg ggacgatcca ccttctattc
     1381 gcatttcgat tcgaaggagg agttgctgtt cgacggcttc gaagcggggt tgcgcgcgtt
     1441 ggcagcgccc ggaccgtccg ggggggcggg gttttgcttc agcctgccgc tgctgcgcca
     1501 catcggcacg cagccgcgct tcgcactggc gacgctgggt cgcagcgcaa actccgccat
     1561 ccagcagacg accaccagga ttctgagcga catcgtccgg ctcgagctcg agcggtcgtc
     1621 cgcctcgtcc ggtgccgcac gtgaagcgga ggtgcacggg atcgtcggag cattccgcgg
     1681 tctcgtcacg tggtggctca cgagcggcag ccggatgagt ccggaagcgc tcgatcaggt
     1741 tttccagcga ctggtgtccg gtatcggtag ggcatgacgc gcgacacgat gctcgatctc
     1801 ggccgggatg ccgtccgccg gcatgcctgg cgcgaggcgt tcacgagtct gtcggccgcc
     1861 gatcaacagt cgccgctgga gcccacggac ctggaagcgc tggcgagcgc cgcccagctg
     1921 cttggccggg atggtgagag cgccgacctc tgggagcgcg cgcatcatgg tttcctgggt
     1981 cgcggagacg tggagcgggc ggcgcgctgc gcgttctggc tcgcctttgg cctcttcgag
     2041 cacggcgaga ccgcccgcgc cggcggctgg ctggcccggg cgcgccgcct gctcgacgac
     2101 ggccggcgcg actgcgtgga gctgggctac ctgctcctgc cgctcgcccg tcagcaggtc
     2161 gctgcgggcg acatcgccgc cgcctatgac agcttcgccc gggccgccga gatcggggac
     2221 cggttcggcg accgcgacct gacgacgctg ggccggcacg gtgtggggcg ggcgctgatc
     2281 cggcgcgggg acatcgccgt gggcgtggcg ttgctcgacg aggtgatggt tggtgtgacc
     2341 ggcggcgagg tggggccggt agtggcgggt gccgtctact gcagcgtgat ctccgcgtgc
     2401 caggacatgt tcgacctgcg gcgcgcgcag gagtggaccg cggcgctgtc gcgctggtgc
     2461 gactcgcaac cggagctggt cccctatcgc ggggaatgtc aggtgcgccg gtcggagatc
     2521 ctgcagatac acggggcgtg gcccgacgcc gtggacgagg cgcgacaggc gtgcgaacgg
     2581 ttgtcgcagc cgccggagca cccgtcgctg ggggcggcgc actatcagct ggcggagctg
     2641 caccggctgc gcggtgagtt cgccgaggcg gaggcggcct atcgcgccgc cagccggtgg
     2701 cgcaagccgc aacccggatt ggcgctgctg cggctggcgc agggccagct cgaagccgcc
     2761 catgcggcga ttcgcggggc cctggacgaa gcctcggggg atgcggcccg ggcgcgtatc
     2821 ctggccgcgt tcgtcgagat cgcgctggcg gcgagcgacc tcgccggcgc cgaggccgct
     2881 gcccacgagc tggccgggat tgccgggcgg ctcgacgcgc gctacctcgc ggcgctgtcg
     2941 gaccagacga gcggggcggt gctgctcgcg caacgccgtc cgcgtgaggc gctcgccgca
     3001 ctccgccggg cctggactgc ctggggtgac ctcgaggcgc cctacgaagc ggcgcgcgtt
     3061 cgcgtcctcg tcgcccaggc atgccggtca cagggggacc gggacacggc ggagctggag
     3121 ctggatgcgg cgcgcgcggc gttcgagcag cttggcgccg cgccggcgct tgcccgcctc
     3181 gaggcgctcg ccggtgtgga ggcgccgccc gcggtgagcc cgttgagcgg gcgcgagctg
     3241 caggtgctcc gtctggtggc gtccgggaag accaaccggg cgatcgccga ggccctcggc
     3301 atcagcgaga agaccgtcgc ccgccacgtc agcaacatct tcaccaagct gggcctgtcg
     3361 agccgggcgg cggcgacggc ctacgcatac cagcacgacc tggtcgtagc gcgctcgacg
     3421 taagagactc ccggtttcga ttccgtcgcg cgtgggccta gtccaggcgc aacgcgaggt
     3481 tcgggtccac ccgtgccgcg cgcagtgccg gaatgaggct cgccagcagg gccgtgaggg
     3541 ccagcacgag ggtgaccgtg ccgaataccg tcgggtcgcg cggcgagacc gcgaagagca
     3601 gcgtgccgat ccagcgcccg ccggccaggg ccagcacggc tccggccgcg atgccgccca
     3661 gtgcgagtcg cagcccgtcg cccaatacca gccacagcac atcgcgcagc cgcgcgccca
     3721 gggcgatccg cacgccgagc tcctgggtgc gttgggccac gttgtacgcg atgacgctgt
     3781 agaggccgat cgccgccacg atcagagcga gccccccgag caccagaaac atcgtggcac
     3841 cggcgcgcca gggacgctgc tcgccgtcca gaatctgcgt gaggggcacg atcgtcacgt
     3901 aggacattcc gggcatttcc tgctgcaggc ggcgacggat cgtcgcgctc gcgtccaccg
     3961 ctcggccgcc gacgcgcacg aacagcccgt acgcgccttc gtccagctga tccgcgggga
     4021 ggtagtaggt cgcggcgccg atttccgtca ggctccgctg cttgatatcc tcgacgatac
     4081 cgaccacggt ccggcacggg gccgtgtcgg cgccgacgcg cacgcactgc cccaatgcct
     4141 tccgcccggg ccagagtcgc tgcgccatgg cctcgctcac cacgattacc ggtggagcgc
     4201 cacgccggtc ctcggcggtg atcccgcgcc cggcgagaat ccgggtcccc agcgtccgga
     4261 agaaatcggg cgagcccgcc tgcagctgaa accgccccag ccgagacacc gaatcgatcc
     4321 ccggcacgaa gagcccgcgc gattcgaagt tgtagaacgg gacgctcacc gcctcggtcg
     4381 cgctcacgac tcccggaagc tcggccgccg cgagcacgag gcggtggttc agcgccgcct
     4441 gctggtcctc gggcaggttc acgccgcgca tgttccgctc cacatacagc accgggtcca
     4501 catcgtaccc caggtgcagc gcccgcacgt tggaaaagct ccggacgaac aacccggcgc
     4561 cgacgagcag cgcgaccgac agggcgccct gcacgacgac gagcagcgcg cgcaggcggg
     4621 agcgttgata gctgccttcg cgagcgccgg ctttgagtcc cgagaccaga tcgccgcgcc
     4681 aggcgggcag cgccggggcg aggccggtga ggacgcccac gacgagggtc aacagcgcgc
     4741 agaacagcat ggtgcgtgta tcggtcagtg gggcgggggc cagatccgtc gtgaagatcc
     4801 gcgtcagcgc cgcgccaccg gcccaggcga gcaccagacc cgccgcgccg ccaaggacgg
     4861 cgagcagcag gctctcggtg aggaggtggc cgagcaggcg gccccggctg acccccaacg
     4921 ccagacgcac ggcgatttcg cgccggcgcc ggaacgcgcg agcgagcagc aggttggcga
     4981 cgttcgccgc ggcgaccagc agcacgacca gcgccacgcc gccgatccag aggccgatgc
     5041 gtgtgcccgg gccggcttcg gggccgcgct ccagctgcgt gggaccgagg atcgcatgcg
     5101 gttgcgcgat gtccagcgcg gccaggctcg ggtccatggc gcgctcggcc agccagctcc
     5161 gccggtaggc ggcggtcagg tcctgggtcg cagcgtcgag gcgcacaccc ggtttcgtgc
     5221 gcaccacgat ctgcagccag ccccagttgt agttcaggta gtagtcgtgc cggcgcatcc
     5281 acgcgtaggc ggtgatgggc acccacgcca cgggccgccg cccatccggc accccgatga
     5341 agccgctggg cgccacgccg atgatcgtga gcgtcgcggg cccgatctgg atcggcatcc
     5401 ccaggatgtc ccgccgcccg ccgtagtgcg actgccagta cccgtagtcg agtaccgcca
     5461 ccggggcgcc ggccggcagg gaatcctcga cgcggtcgaa gaaacgcccc agcaccggtc
     5521 gggcgtcgaa gaaggcgaag aacccggcgc tcgcggtccc taccgacgcc tccgtgatgt
     5581 cgggcccggt gccgagcgcg gcgggaaagg tggcgaacgc ggcgacctgg gcgaacgagc
     5641 gcgtcgcgct cgtcaggtcg aggtaacgcc tgaattcgaa cgtgtgggag gtgaacggct
     5701 gcccgcggaa cgtggaggcg agatacacgc ggtgcacgga acggggctcg tgcagatatg
     5761 cgggagggcg gaacagcagc agatcgagca cgtcgaacac cgcggcgttc gccccgatgc
     5821 cgagcgccag cgtcgccacg accgcgagcg tgaatcccgg cgacaggcgc agcccccgcg
     5881 cggcataccg gagatcgtgg gtgacggcgt cgagcagcat cagaccgcga gcatcccggg
     5941 cgtcgtcctt gatccgctcc acgcctccga aggcggcgcg cgccttgcgg agggcctccg
     6001 cgggcggatc gcccgcgcgg acgtactgct ccgcctgctg ctcgatgtgg aatcgcagct
     6061 cctggtcgag gtcctgctcg agcgcatccc ggcgcagcag ggcgcgcagg cgatagcgca
     6121 ggtcgctcag cagctcggcg agcatctcat cccatctcca tgacgaggcc gacggcgtgc
     6181 gtgagcttgc tccaggctcg ggcttcggcg tcgagctgct tgcgaccggc ggacgtcagc
     6241 gcgtagtact tggcgcggcg gttgttgtcg gacgtccccc aggtggcggc gatccagccg
     6301 cgacgctcga gccggtgcag ggcggggtag agcgagcctt gcgggacctg caacgtggcg
     6361 cgcgagacct ggtgcagccg ctcggagatg gcccagccgt gttgcggtcc gagcgacagg
     6421 gccttgagga tcaacaggtc gagcgtgccc tggggcaggt cgagacggtc ttcggtcacc
     6481 ggtcttctcc cctttcgctt ctacaagaga gtacggctgt tcctgtagaa gtgcaagggg
     6541 gcgcccctac atagaactac ccatcgcggt gcaggggtgg ggctggatac ttcgtccgat
     6601 gcggccggcc ggggctcgga tgagattcac gctcgactca ttcaacgagg tacgcgatga
     6661 gcagccggca tgagatagag cggatccaga ccgtcgtcat cggtgggggc caggcgggac
     6721 tgtcggtggg ataccagctg gcgcggcgcg ggctgccgtt cctgatcctc gatgccaatg
     6781 cgcgggtcgg ggactcgtgg cgccgccgct gggactcgct tcgcctgttc acgccggcgc
     6841 ggtacgacgg cctggcggga ttcccgttcc cggcgaagcg tgacgcgttc attaccaagg
     6901 acgagatggc cgattatctc gaggcgtacg ccgcccgctt ccggttgccg gtgcgaaatg
     6961 gcgcccgggt ggatggcctg tcgcgccggg gggaccggtt cctcgtcacg gcgggcgagc
     7021 ggcgctacga ggccgaccag gtggtcgtcg ccatggcgaa ctaccaggtc ccgcgggtgc
     7081 ccgagttcgc gtcggcgctc gaccggggca tcctccagct gcattcacgc gagtaccgga
     7141 acccgtcaca gctggcgcgc ggcggcgtgc tcgtcgtcgg cgtggggaat tccggggccg
     7201 atatcgcgtt ggaggccgcc cggacccacg cgacctggct ggcggggaag gaggccggcc
     7261 acgtaccgtt tcgcatcgag cggtggctgg cccggcattt cctggtgcgg gtcgtgcggt
     7321 tcgtcggcca tcgggtgctg acccggggca cgccgatcgg tcgcaagctg acgcccacat
     7381 tgctgcaccg cgccgcaccg ctcgtacggg tcaagccggc ggacctcacc gccgccggca
     7441 tcgtccgtgt gccccgggtc gtcggggtgc gggacggccg cccgctgctc gcggatgatc
     7501 gcaccctcga tgtggccacc gtgatctggt gtacgggatt ccatcccggc ttctcgtgga
     7561 tccagcttcc cgtgctgggg gacgacgggc tgccgcggca tgaacgcggg gtcgtcgtcg
     7621 agcagccggg gctgtatttc gtcggcctgc atttcctcta tgcgatgtcg tcggacacgg
     7681 tgaacggggt ggggcgtgac gcggagtgga tcgcccgggc catcgcggtg cgcgtacgcg
     7741 atgcccgctc ggtgcccgcc gccaatccgg ccgccgtcgc cgcgtgaagg ggagacctgg
     7801 cggagcgtta ccgctgcgac gccggccgca gccccagcgc gccgttgagc cagcgcacca
     7861 gcgggagcat caggaggaac tgccgctcca gcagtgtgac caagcggggg ctcaacgcgt
     7921 ctgtgtcgct cagcgtgcgc ccggcggtga aggactggag ccgcagccac gtcgcggccg
     7981 ggtggtccgg tgggtatccg cgtggcgtcc gcttcagcgc gtcctcgcgg gtcaggccgc
     8041 cgagacggcg gcgcagcgcg gggtggtcgg cgatacggaa gaaggcccgg ggattctcgg
     8101 cgacggcgtc gcggatgcgg gcgagggcgg cacgaggcgg catccagatg ccgccgcccg
     8161 tgaacgagtc tccgggcgcg acctgcacgt agaatcccgc gctcccgccc tcgccatcct
     8221 ggcccacctt tcgggtggcg tcgcggtggt agaaccaggc cccgatgtgg gtcttgtagg
     8281 gcgatttgtc gcgcgagaac cggatgtcgc ggttgatgcg aaagatcgac cgcctggggt
     8341 cacccacgat ctcgggggcg aaccgcgcca ggcgcacgtc catctcctcc accagctccc
     8401 gcatcggccc gcggacatgc tgttcgtagt cgtcgcggtg cgcctcgaac cacggctttt
     8461 cgttgcgccg ggcgagaccg cggaagaacc gcagggtttc ggaggagaac cccatagcgg
     8521 aatctgccgc cgggaacggc ggccatcaag gcttcttgct caccaccgac tcgagaatcg
     8581 ccaccgcgcg ttgcagcgcc gcgaggtcgg ccgccgcaag gtcggtgagg agggcgcgga
     8641 gcgcggacag gcggcgggcg cgggccttgg cgagcacgcg ctcgccggcc ctggtggcgc
     8701 ggatccggac gatccgcgcg tcttccgggg caggggtccg ggtgacgagg ccctcggcct
     8761 ccatcgcttg gaccaggcgc gtcatcgtcg gaggccggac gtgctcggcg gccgccagct
     8821 cgcccacggg tcgtggcccg ccgcccagga ccagcaccga tagcaccgag agccgtggtc
     8881 cggtgacgcc ggactgctga tcgctccggg ccacgcgacg caggagatga atggccagcg
     8941 aatgcaggcg atcggcgatc gccgcacggt ccgtcatgag gtctccccct cgggtcttgc
     9001 gcgtccgtcg ctcggtcatt atttaggtac cctaactaaa ttatgaccgg cgtgagcgcc
     9061 gcgcaatccc ggcggcgcgc gtcggtagag gaggctcgca tgccgactgt cgtcgacctt
     9121 agtgtggcga tccgcgaccg gagtgtcccc cgggtggccg cgatccttcg cgaagccccg
     9181 gccctcgccg ccgcgcggcc ggcgggaaat cccagcccgc tgctgctcgc ggtgtacgtg
     9241 aatgcgccgg acatcctcgc attgctccgc ccgcacgtga tgctcgatgc ctgtgaggcg
     9301 gcggcggcgg gcgacgtgga gcggttgcgc acgctcctcg ccggcgacgc cgcccaggtg
     9361 tacgcccgca gcggcgacgg ctggacgccg ttgcacctcg ccgcgttctt cgggcggcgc
     9421 gaggcggtgg agctgctgct cgcgagcggg gccccgatct cggccgtgtc cggcggacag
     9481 gagcgcaatc agccgctcca tgcggcgctg gccggggcgt gcgaccgggt gatcgtgcgc
     9541 gccctagtca tggcgggcgc ggacgtcaac gcggtggggg cgatgggggt gacgcctctg
     9601 catgaggcgg cgtcccgcgg caatgcagag ctggtgcggt tcctgcgagc cgccggcgca
     9661 aacgtgatgg cgacgctcga cgacgggaag acaccggcac agctcgcggc ggcgcgcggc
     9721 catgccgcgc tggccgagga gctgcgggcg ctggtctagc cgcggatgcg ggtgctcatc
     9781 ccgccgcgcc gaagagcgcg ccggcgccgg cggtgagcgc catcgccagg gcgccccaga
     9841 acgtcacacg cagcgtggcc gtggccaccg gagcgccgcc ggtgcgggcc gagaccgcgc
     9901 cgagaccggc gaggaaggcc agggaggaga gggcgacgac cgggagcatg gtgccggagg
     9961 gcgcgagcag cgcggcgagc agcggcagga aggccccgac ggcgaaggtg gccgcggagg
    10021 cgagcgccgc ctggatcggc cgcgcccgat gcatgtcgac gatgcccagc tcctcgcgcg
    10081 catgcgcccc gagcgcgtcg tgggccatca gctggtcggt gacctgttcg gcgagcgccc
    10141 gggtgagccc acgggcttcg tagatcccga tcagctccgc ccgctcgtgc gccggctggc
    10201 tcgccagctc gtgctgctcg cgggcgagat cggcccgttc ggtgtcggcc tgggagctga
    10261 ccgagacgta ctcgcccgcc gccatcgaca tggcgccggc gacgagcccg gcgacacccg
    10321 cgacgagcac ctggtgagga gaagcctcgg cggccgcgac gccgaggatg aggctcgcgg
    10381 tggacacgat cccgtcgttc gccccgagta ctgccgcccg cagccagccg atgcgtccgg
    10441 aacgatggac ttcgacgtgg cggggaggca tgcgcggcgg cgcccgaccc cggctaggcg
    10501 gcggcgcgcc gtgccggccg agtcagcttg gccaggtact ccttggcggc ctggagcgcg
    10561 cggcgcttcc cgccccagga gaggtcggcg aagaaccggc tgtgccgcgg gacgtacatc
    10621 ccgtcccgcc gccgatggaa cccggcgcgg acataccagc cgtgggtacg ggtggagtcc
    10681 tggtcgatcc ggctgatgcc gcggcgtcgc accggccggc gggcccgacg tttcgtagta
    10741 gtcgctttca tgacaggctc ttgcggctga ggttaggcgt gaaaactgca ccgcgccttg
    10801 acctgtggct agcccgaggt gggggcaaga aaccttcaga ctcactcggc gcgcagcgcc
    10861 cgcacggggt ccacgcggga ggcccgccgc gccgggacca ggctcgccac gagggcgacg
    10921 gcgatgagca gggcggtcac ggcggcgaac gttgccgggt ccgtcggact ggtctcgtag
    10981 aggagcttcg acagggcgcg cgtcgcgatg agcgcgccgg tggtgcctgc cacgaccccg
    11041 agtccggcca ggcgtaacgt ccgggtcaac accaggcgca tcacccggcc gatcggggcg
    11101 ccgagggcca ggcggatgcc gatttcgtgg gtccgctcca tgacggagta ggccagcagg
    11161 ccgtagatgc cgaccgcggc cagcgacagc gccagcagtg agaagaccac gaggatgcgc
    11221 gtctggaaca gcggcgtggc ccgaatgccg gcgatgaagt cctgcagcga agccagggct
    11281 tgcggcggga ggtcggggtc gatgtcccgg agcgtcgcgc ggacggctcg cgcgtactcg
    11341 ccgggggcgg cgtcggtgcg ggcgaggaac gtcatgtggc tcaggaagaa cgtcagcgag
    11401 acctgattga gcggttggta gatcgccggc acggggccct gggtgagccg tt
//
LOCUS       DBNGZW010000046         2216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_822500, whole genome shotgun sequence.
ACCESSION   DBNGZW010000046 DBNGZW010000000
VERSION     DBNGZW010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_822500"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="AC16JC_01225"
     CDS             complement(<1..297)
                     /locus_tag="AC16JC_01225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HIR5163347.1"
                     /translation="MGVSVAAARSIAERFALEGEIVSVVRLAGGHINDSYRVELRAGD
                     AGRVYLLQRLNPRVFPHPDLVMENVSRVTRHLSSRLAAAAVADPERRALALVPTR"
     gene            complement(288..1190)
                     /locus_tag="AC16JC_01230"
     CDS             complement(288..1190)
                     /locus_tag="AC16JC_01230"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5163348.1"
                     /translation="MDLQLVILAAGLGSRYGRMKQLEGVGPGGETLMEYSLYDALRAG
                     FGEAVFVIRADMEEAFAEFATARFGNRIPWRPAIQRLEDVPSGVLVPPDRKKPWGTAH
                     AVLAAADCVHAPFAVLNADDFYDAPSFSALAGHLREHAGARPPSYAVVGFRLGDTVPE
                     SGGVNRGWCRADSSGWLESVEEVLRIEAAPGGFAGAGESGPVLLPADALVSMNLWGFT
                     PVVFDTLRRGFAEFFRSGEPARGEYLIPTAIRAALERRECRVRVLDPGSRWFGVTHPA
                     DRPVVAAALQQLVRDGRYPERLWE"
     gene            1256..1645
                     /locus_tag="AC16JC_01235"
     CDS             1256..1645
                     /locus_tag="AC16JC_01235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163349.1"
                     /translation="MPIAYTIDHAHRLVMAAGHGELTGDDIFGYQTEVWSRADIGGYD
                     ELMDMTDVQRIDLRSVDHIKQLAELSASMDPAAVQSRFAIVAPGDYAFGLGRMYESYR
                     RLEGRSTKRVGVFRSMAEARAFLELDD"
     gene            complement(1642..>2216)
                     /locus_tag="AC16JC_01240"
     CDS             complement(1642..>2216)
                     /locus_tag="AC16JC_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR5163350.1"
                     /translation="LDGIARSGVRSRVDVIVVSDHGMSPTTPDSVVALDEYLPLTLLT
                     RSVGGNPLYGLWPRPGMEDSVYRALHGRHPHLAVWRRSEIPARFHYRDNRRIAPILAL
                     VDPGWSLALQRSEVIQHPERFRGGAHGYDDTVSVMRAVFLGAGPAFREGYVAAPFRNI
                     HVYDLIARILGLTPAPNDGSADSTASLLRP"
BASE COUNT          362 a          797 c          758 g          299 t
ORIGIN      
        1 gcgagtcggc acgagcgcca gcgcgcggcg ctccgggtcg gcgacggccg ccgccgcaag
       61 acgggaggac aagtggcggg tcacccgcga cacgttctcc atcacgagat cggggtgcgg
      121 gaagacgcgc ggattcaggc gttgcagcag gtagacgcgg ccggcgtcac cggcgcggag
      181 ttccacccgg taggagtcgt tgatgtggcc gcccgcgagc cgaaccaccg agacgatctc
      241 tccctcgagc gcgaaccgct cggcgatcga ccgcgccgcg gcgacgctca ctcccacagc
      301 cgttccggat agcggccgtc ccgaaccagc tgctgcagcg ccgccgcgac caccggccgg
      361 tccgccggat gggtgactcc gaaccagcgg cttcccggat cgagcacccg gacccggcat
      421 tcccgtcgct cgagcgccgc ccggatggcg gtcgggatca ggtactcgcc gcgcgccggc
      481 tcgcccgagc ggaagaactc cgcgaatccg cggcgcagcg tgtcgaacac caccggcgtg
      541 aagccccaga ggttcatcga gacgagggcg tcggcgggca gcaggaccgg ccccgattcc
      601 cccgctcccg cgaagccgcc gggcgcggct tcgatccgga gcacctcttc cacgctctcg
      661 agccagccgc tggaatcggc ccggcaccac ccgcgattca ccccacccga ctccggcacg
      721 gtgtcgccca ggcggaagcc caccaccgca tagctcggcg gccgggctcc ggcgtgctcc
      781 cggagatgcc cggccagggc cgagaatgaa ggcgcatcgt agaagtcgtc ggcattgagt
      841 acggcgaacg gagcgtgcac acagtcggct gcagccagca ccgcgtgcgc ggtcccccac
      901 ggtttcttcc ggtccggcgg caccaggacc ccggacggga catcctcgag gcgttggatc
      961 gcgggacgcc agggaatccg attcccgaat cgggcggtcg cgaactcggc gaacgcctcc
     1021 tccatgtcgg cgcggatgac gaagacggct tcgccgaacc cggcccggag agcgtcatag
     1081 agggagtact ccatgagggt ctccccaccg gggcccaccc cctcgagctg cttcattcga
     1141 ccgtagcggc tccccaaccc ggccgcgaga atgacgagtt gtaggtccac tggtcggccg
     1201 tcccggctgg tgcttgtcgg tgcccgcctc gtgcgccagt ttgaaagtac cacgcatgcc
     1261 catcgcgtac accatcgacc acgcccaccg cctggtgatg gccgccggcc atggcgaact
     1321 caccggcgac gacatcttcg gctaccagac ggaagtctgg tcccgcgccg acatcggcgg
     1381 gtacgacgag ctgatggaca tgaccgacgt ccagcgcatc gacctgcgct cggtggacca
     1441 catcaagcag ctcgccgagc tgtcggcctc gatggacccc gcggccgtgc agtccaggtt
     1501 cgccatcgtg gcgccgggag attatgcgtt cgggctgggc cggatgtacg agagctaccg
     1561 ccgcctcgaa gggcgcagta cgaagcgggt cggcgtgttc cgctcgatgg ccgaggcgcg
     1621 agcgttcctg gagctcgacg actagggacg cagcagcgag gccgtggaat cggcgctgcc
     1681 gtcgttcggc gccggcgtga gcccgagaat cctcgcgatc agatcgtaga cgtggatgtt
     1741 gcggaacggc gccgcgacgt agccctcgcg gaacgccggc cccgcgccga ggaagaccgc
     1801 ccgcatcacg gataccgtgt cgtcgtagcc gtgtgccccg ccacgaaacc gctccggatg
     1861 ctggatcacc tccgagcgct gcagagccag cgaccacccg ggatccacta gggcgaggat
     1921 cggcgcgatg cgccggttgt cgcgatagtg gaaccgggcg gggatctcgg acctccgcca
     1981 cacggcgagg tgcggatgcc tgccgtgcag ggcccgatac acgctgtcct ccatccccgg
     2041 cctgggccag aggccgtata gcgggttccc tcccacgctc cgcgtgagca gcgtgagcgg
     2101 cagatactcg tccagcgcca cgacgctgtc cggcgtcgtc ggcgacatgc cgtggtcgga
     2161 gacgacgatg acgtcgaccc tggagcgcac gccgctgcgc gcgatgccgt cgagca
//
LOCUS       DBNGZW010000047         7185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_549710, whole genome shotgun sequence.
ACCESSION   DBNGZW010000047 DBNGZW010000000
VERSION     DBNGZW010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7185
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_549710"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1062)
                     /locus_tag="AC16JC_01245"
     CDS             complement(37..1062)
                     /locus_tag="AC16JC_01245"
                     /inference="COORDINATES: protein motif:HMM:NF021778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR5163351.1"
                     /translation="MPRIHPSLALALGAGFLLACSQDRPTSTLESLPPASGPLLASAS
                     NSCGGNTHPAGTIVDTVVTPPAWGIAVRDDGLAYFTEPYNGGVGITSTRTRAVDGFIA
                     TGSIPTGVAFSPDGSRAYVANQLDNNVSVINVATAQVIATISTGFDATFSVQVSLDGS
                     RLFIGTNGTTVYIVDTQTNQIIGSAQVGAAPNAMVVAPDNRIIYVSSFVGGTVTELDL
                     FTGAVLRTFFVGGTPQGMAITRRGDRLYVANEAGYLSEITLQTGAITATMPLAGGGFG
                     VGVTADDVEAWVTIPFGGAVQVFNLQNHRLSQAFSVGGEPRRVAFSQKGAIGAIANLA
                     GFITLVR"
     gene            complement(1351..1947)
                     /gene="msrA"
                     /locus_tag="AC16JC_01250"
     CDS             complement(1351..1947)
                     /gene="msrA"
                     /locus_tag="AC16JC_01250"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007353945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR5163352.1"
                     /translation="MNPTLLLVLLAGLGRPAVAPQTQRAVFAGGCFWGVDAVFKHVRG
                     VVQVVSGYAGGNAADPTYGLVSTGTSGYAESVEVTFDPAQVSYEQLLDVFFTVAHDPT
                     ELNRQGPDVGTQYRSAIFYVDSTQRRSALHSIAQLNAQHVYPAPIVTEVAALTAFHPA
                     EAYHQDYLAHHLTQPYIVYNDLPKLERLKEKFPQLYRQ"
     gene            complement(1973..2863)
                     /locus_tag="AC16JC_01255"
     CDS             complement(1973..2863)
                     /locus_tag="AC16JC_01255"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014433.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR5163353.1"
                     /translation="MTTRTHVFLGKGGVGKTTCAAATALAYADAGERSLAISTDPTPS
                     LSHIFELPGRQHDREVRDGLVLTELGLEEVRAMWDDRFGREVYAVFSAFVDIEYERFV
                     EFMTSVLPGLNEEFMVDHIRRLALQGAYRHIVWDTAPLGQTLALLGTPSLLAEHLRLA
                     PRVYSHLRTTGERRESVMAALQRWDELAQTCMAFLRDEVRFSMVTIAEALAVRQLEPV
                     FAELARHGLRVQRLIVNNVVQTVDSAFLRQKAEHQVPHLEFLREHYGHLRIVEIPLLP
                     YEVRGMDRLREVARVVGPDR"
     gene            complement(2898..3299)
                     /locus_tag="AC16JC_01260"
     CDS             complement(2898..3299)
                     /locus_tag="AC16JC_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163354.1"
                     /translation="MKTTWVLGLVALTGVASITTAATRTDASAAPDPRSAIFIRRGCN
                     ECHAISVFRIRAVHDVAPDLTYAYGDVVLRYGVDLERFLDNPSGVMRLVLGSHIRLSP
                     ADRDSIVRILVGMYAQRVASRARGDSSLRSE"
     gene            3427..4815
                     /locus_tag="AC16JC_01265"
     CDS             3427..4815
                     /locus_tag="AC16JC_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5163355.1"
                     /translation="MPTPIRILSALCIAATPAAGAQARIDQSKLQDEAVHLITEYVRI
                     NTTNPPGNEAAAMLFFARIFRDEGIVFDTASSAPGRGNIWARLKGGSAPALVLLHHMD
                     VVPADTHYWHADPFGGTVTSGDLMGRGTLDTKTLGIVQLEAFLALHRRHAPLSRDVIF
                     LATADEEAGGAFGAGWVVKHHPEAFAGAGFVLNEGGDGSVEDGRARFGIEVTQKVPLW
                     LRLVATDRPSHGSIPPIASSVNRLVRALFRIQTHDFAPRIVPAVDAYFKGLGPSAAPQ
                     WRDAYGDIARAVQDRNTLLRLQIDAPAMAALTRNTCSITMLAGSNKINVIPPEAQAQL
                     DCRLLPDQDRATFLAELAAVIDDPGIRIEQIMGFTPAVSPSDNPLYRAIADVTRKHFP
                     AASIVPQVSTGFTDSHFFRDLGLASYGYAPFLIPADQEGGVHGNDERISLENVRRGTV
                     MMLEIVEAMTRP"
     gene            5105..6841
                     /locus_tag="AC16JC_01270"
     CDS             5105..6841
                     /locus_tag="AC16JC_01270"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012692830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004467 - long-chain fatty acid-CoA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HIR5163356.1"
                     /translation="MTAPERIATPGTSRVWHAAYDAGVPPSLAYEPLTVPQLLEQAAA
                     RYPDRPAAVFLNCRLTYAELYEDVRRLATALAQLGVERDTRVAIQLPNIPQAVIAYYA
                     TLSLGAQAVMTNPLYVQREIEHQWTDADCRVAFVTDFLYERTIRSLRDRLPVRQYVVA
                     SIPEYLRFPLSVLAPLKLRRMRPPAFARVAPERGVHFFRSLVQRTPADPPRPEIGLDD
                     LAVLQYTGGTTGVAKGAMLTHRNLSSNVQQIRAWLPDLALGREVFLTALPLFHSFGLT
                     VCMNLPVYAGAVMVLVPNPRDIATVLAGLERGVTIFPAAPALFNAILNHPRLRPERLR
                     HIRYCVSGAAPLPVELLRRFEEATGGIIAEGFGLTETSPVTHVNPLKGKRKIGSIGIP
                     LPDTDAKIVDLADGRTAMPVGQEGELLVRGPQVMKGYWQRPDETALTIRDGWLHTGDI
                     ARVDEEGYTTIVGRKKEMIIVRGFKVFPDEVDGILARHPAVLESATIGIPDAQSGEHV
                     RAVVVLRPGHIATSEDLIAHCRTELAPFKVPKEIEFRAELPKSAAFKVLRRELRAEAI
                     AALTGASGAAAR"
     gene            complement(6810..>7185)
                     /locus_tag="AC16JC_01275"
     CDS             complement(6810..>7185)
                     /locus_tag="AC16JC_01275"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5163357.1"
                     /translation="LGRVHTQRGEYAEAERALREAVSLSPETAYATGALGYLYAVRGD
                     RAAAERIVEELEAHRKTGYVSPVAIGMVYAGLGMADEVFAWLERAYEERRGWLAYLKV
                     EPTLDGLRGDPRFTELLRRMRL"
BASE COUNT         1186 a         2470 c         2409 g         1120 t
ORIGIN      
        1 gtcccgggac ccgacttcaa gcgggagcga cgatgcttac cgcacgagcg taatgaaccc
       61 ggccagattt gcgatcgccc cgatcgcgcc cttctggctg aaggcgaccc ggcggggctc
      121 gccgcccacg ctgaacgcct ggctcagccg atgattctgg aggttgaaga cctgcaccgc
      181 tcctccaaag gggatggtga cccacgcctc cacgtcatcg gccgtcacgc ccactccgaa
      241 cccacccccc gcaagcggca tcgtggctgt gatggcgccg gtctgtaacg tgatctcgct
      301 caagtagccc gcttcgttcg ccacgtagag gcggtcgcct cgacgcgtaa tggccattcc
      361 ctgcggcgtc cccccgacaa agaaggtgcg caataccgca ccggtgaaca ggtcgagctc
      421 cgtaaccgtg ccgccgacga aactgctgac gtaaatgatg cggttgtcgg gtgccaccac
      481 catcgcgttc ggcgccgctc cgacttgcgc gctgccgatg atctggttgg tctgggtatc
      541 gacgatgtac acggttgtgc cgttggtgcc gatgaacagc cggcttccgt ccagcgacac
      601 ctgaacggaa aaggtcgcat cgaagcccgt ggagatggtg gcgatcacct gggccgtcgc
      661 cacgttgatc accgatacgt tgttgtcgag ctggttggcg acgtaggccc ggctcccgtc
      721 cggggagaac gccacgccgg tggggatcga ccccgtggcg atgaagccgt cgaccgcccg
      781 tgtcctggtg ctggtgatgc ccacgccccc gttgtacggc tcggtgaagt aggcgagccc
      841 gtcgtcccgc accgcgatcc cccaggcggg cggcgtcacg acggtgtcca cgatggttcc
      901 ggcggggtgg gtgttgccgc cacagctgtt cgaggccgac gcgagcagcg ggccgctcgc
      961 cggcggcaac gattcgagcg tactggtcgg gcggtcttgc gaacaggcga gcaggaagcc
     1021 cgcccccagg gcgagcgcga gagacggatg gattcgtggc acggtacctc cgggaggaaa
     1081 aggtccccaa cgtggtcgag aacgtcgcag gagcctcgcc tgacgaaccg gcggagcgcg
     1141 agctggaggt gtgactcgcg gtttcttctt tagaggcgct cgggataccc caccgaagct
     1201 ccttgcacgg gacggcccgt ggacaaacgg gaggaactgc gagcggacga aactggtgag
     1261 aggatcgagc cgcgtcaatc agcgaatggc tgacacgatc tcgtccgcgt gtaggacgga
     1321 gtcctacagc ggagtctccg aagctggcgg ctactgtcga tacaactgtg ggaacttctc
     1381 cttgagtcgt tcgagcttcg ggagatcgtt gtagacgatg tatggctgcg tcaggtggtg
     1441 ggccaggtag tcctgatggt acgcttcggc ggggtggaac gcggtcaggg ccgcgacctc
     1501 cgtcacgatc ggcgccggat acacgtgttg cgcgttcagt tgcgcgatgg agtgcaatgc
     1561 gctgcgacgc tgcgtgctgt cgacatagaa gatggccgat cggtactgcg tacccacgtc
     1621 gggcccctgg cggttcagct ccgtgggatc gtgggccacg gtgaagaaga cgtccaacaa
     1681 ctgctcgtag gagacttgcg ccgggtcgaa ggtgacctcc accgattcgg catagccgct
     1741 ggttcccgtg ctgaccagcc cgtatgtcgg gtcagccgcg ttgcctcccg cataccccga
     1801 gaccacctgc accaccccgc gcacgtgctt gaacacggca tccactcccc agaaacagcc
     1861 gccggcgaat acggctctct gggtctgggg agcgacggcc gggcggccga ggccggccag
     1921 caggacgagc agcaaggtgg ggttcatgga ggacacgtac actcttggaa cgctatcggt
     1981 ccggacccac tacccgggcc acctcccgca gccggtccat gccgcgcact tcataaggaa
     2041 gcagtgggat ttcgacgatg cggaggtggc catagtgttc ccgcaggaac tccagatgcg
     2101 ggacctggtg ctcggccttc tggcgcagga acgccgaatc caccgtctgg acgacgttgt
     2161 tcacaatcag gcgctgcacc cggagcccgt gccgcgccag ctccgcgaag acgggctcga
     2221 gttgccgcac ggcgagcgcc tcggcgatcg tcaccatcga gaagcgcacc tcgtcgcgca
     2281 ggaacgccat gcaggtctgc gccagctcgt cccaccgttg cagcgcggcc atcaccgact
     2341 cgcgccgctc ccccgtcgtg cgcaggtgcg agtacacgcg cggcgccagc cgcagatgct
     2401 cggcgagcaa ggacggcgtc ccgagcagcg ccagcgtctg ccccagggga gcggtgtccc
     2461 acacgatgtg gcgatacgcc ccctgcaagg cgaggcgccg gatgtggtcc accatgaact
     2521 cctcgttgag ccccggcagg accgacgtca tgaactcgac gaagcgctcg tactcgatgt
     2581 ccacgaacgc cgagaagacg gcgtacacct cccggccgaa ccggtcgtcc cacatcgccc
     2641 gcacttcctc gagacccagc tccgtgagca cgaggccgtc gcgcacctcg cggtcgtgtt
     2701 ggcgtcccgg gagctcgaag atgtgggaga gcgacggcgt gggatcggtg gagatggcga
     2761 ggctgcgctc gcccgcgtcg gcgtaggcga gggccgtcgc cgccgcacag gtggtcttgc
     2821 ccacgccccc cttgcccagg aagacgtggg tgcgcgtcgt cattccgact tgcccaggaa
     2881 gacgcgggtg cgcgttgtca ttccgagcga agcgaggaat ctccacgcgc gcgcgaagcc
     2941 acacgctgcg cgtacatccc cacgaggatc cgcacgatcg agtcgcggtc cgcggggctg
     3001 agtcggatgt gggaaccgag caccagccgc atgactccgc tgggattgtc gagaaaccgt
     3061 tcgaggtcga cgccgtagcg gagcaccacg tctccgtatg cataggtgag gtcgggcgcg
     3121 acatcgtgca cggcccggat ccggaatacg gagatcgcat gacactcgtt gcagccgcgc
     3181 cggatgaaga tcgccgaacg cggatcgggc gcggcggacg cgtccgtgcg agtcgccgcc
     3241 gtcgtgatcg aggccacacc cgtgagggcc accaggccca gtacccaggt cgtcttcata
     3301 gagcctcctc taccattgta gaggcggggg gcgtggaatc gaatgaaggt cggacgaacg
     3361 gttcttcagg ccggagcggg aagggtggtc cctggcgctc ccccgggttg gacggcacgt
     3421 taccggatgc ccactcccat tcgcatcttg tccgcgctgt gcatcgccgc gacccccgcg
     3481 gccggcgccc aggcgcggat cgatcagagt aagctgcagg acgaagcggt acacctgatc
     3541 acggaatacg tccgcatcaa caccacgaat ccgccgggca acgaggcggc ggcgatgctg
     3601 ttcttcgctc gcatctttcg tgacgaaggc atcgtgttcg acacagcgtc gtccgcaccg
     3661 ggccggggca acatctgggc gcgcctcaag ggcggctctg cacccgccct ggtgctgctg
     3721 caccacatgg acgtcgtgcc ggccgacacc cactactggc atgccgaccc cttcggcggc
     3781 acggtcacga gcggcgacct gatgggacgc ggcacgctcg acaccaagac cctgggcatc
     3841 gtacagctgg aagcgttcct ggcgctgcac cgccggcacg cgccgctcag ccgggacgtg
     3901 atctttctcg cgacggccga cgaggaagcg ggcggcgcgt tcggagccgg ctgggtggtg
     3961 aagcaccatc ccgaggcgtt cgccggtgcg ggcttcgtgc tgaacgaagg cggcgacggc
     4021 tccgtggagg atggccgcgc tcggttcggc atcgaggtga ctcagaaggt gccgctctgg
     4081 ctgcgactcg tcgcgaccga ccggcccagc cacggctcca ttcctcccat cgcctcgtcg
     4141 gtcaaccggc tggtccgggc gctgttccgc atccagaccc acgacttcgc gccgcgcatc
     4201 gttcccgcgg tggatgccta cttcaaggga cttgggccga gcgccgcgcc ccaatggcgc
     4261 gacgcatacg gcgacatcgc gcgggcggtg caggatcgca acacgctgct gcgactgcag
     4321 atcgacgcgc cggccatggc ggcgttgacg cgcaatacct gctccatcac catgctcgcg
     4381 ggcagcaaca aaatcaacgt gatccctccc gaggcgcagg cgcagctcga ttgccgcctg
     4441 ctcccggatc aggaccgcgc caccttcctg gcggagctcg ccgcggtgat cgacgacccc
     4501 ggcatccgga tcgagcagat catggggttc acgcccgccg tctcgcccag cgacaacccg
     4561 ctctaccggg cgatcgccga cgtgacccgg aagcatttcc ccgccgcctc catcgtcccc
     4621 caggtctcga ccggcttcac cgacagccac ttcttccggg acctggggct ggcgagctac
     4681 gggtacgcgc cgttcctcat ccccgccgac caggagggcg gcgtgcacgg caacgacgag
     4741 cggatctcgc tcgagaacgt gcgccggggg acggtcatga tgctggagat cgtggaggcg
     4801 atgactcggc cctaggaaag ctctgcgtac ctggtgcgac gcagggggtg ggggcgcccg
     4861 gacgcgttgg acgcgggggc gctcgacccg gaccagcctt ccggatctcg ttgccggcca
     4921 gcagcgaaaa tgtccgggcc gtgcggcccc cgcgtcctcg cggtccgggt gcccccaccc
     4981 cccacgtcgc acggaatgtg aagagctttc ctggggcccg tttgtttcac gccaatctca
     5041 ggaggtgttc agccccgacg tgctaagagg aaagcatccc ctgcatggag cgcccgatgg
     5101 ggtagtgacc gcaccagagc gaatcgcgac acccggtacc tcccgggtct ggcacgccgc
     5161 ctacgacgcc ggcgtcccac cctcgctcgc ctacgagccg ctgaccgtac cccagctgct
     5221 ggaacaggcg gcggcgcgat atccggaccg gcccgccgcg gtgttcttga actgccgact
     5281 cacctacgcc gagctgtacg aggacgttcg ccggctcgcc accgcgctcg cccagctcgg
     5341 cgtcgaacgc gacacccgcg tcgcgatcca gcttcccaac atcccgcagg cggtcatcgc
     5401 ctactacgcg acgctctcgc tcggtgccca ggcggtgatg acgaacccgc tctacgtgca
     5461 gcgggagatc gagcaccagt ggaccgacgc cgactgccgc gtggcgttcg tgaccgactt
     5521 cctctatgag cggaccatcc ggagcctgcg cgaccgcctg ccggtgcggc agtacgtggt
     5581 cgcctccatt ccggagtacc tgcggttccc gctctcggtc ctggcgccgc tcaagctgcg
     5641 ccgcatgcgg ccgccggcct tcgcacgggt cgcgccggag cgtggcgtgc acttcttccg
     5701 gtccctggtc caaaggacac cagccgatcc accgcgcccc gagatcggtc tggacgacct
     5761 cgccgtgctg caatacacgg gcggcacgac cggcgtggcc aaaggcgcca tgctgacgca
     5821 ccgcaacctg tcgagcaacg tgcagcaaat tcgtgcctgg ttgcccgatc tcgcgctggg
     5881 gcgcgaggtc tttctgacgg cgctgcccct gttccacagc ttcgggctga cggtctgcat
     5941 gaacctcccg gtgtacgcgg gcgccgtgat ggtgctggtc ccgaacccgc gcgacatcgc
     6001 gaccgtgctc gcgggcctgg agcgcggcgt caccatcttc cccgcggccc cggcgctgtt
     6061 caacgcgatc ctcaaccacc cgcggttgcg gcccgagcgt ctgcgccaca tccgctactg
     6121 cgtctccggc gcggcccccc tgccggtcga gctgctgcgg cgcttcgagg aggcgaccgg
     6181 agggatcatc gcggaggggt tcgggctcac ggagacgtcg cccgtcaccc acgtgaatcc
     6241 gctcaaggga aagcgcaaaa tcgggagcat cgggattccg ctccccgata ccgacgcgaa
     6301 gatcgtggat ttggcggacg gccgaaccgc catgccggtg gggcaggaag gggagctgct
     6361 cgtccgtggc ccgcaggtca tgaagggcta ctggcagcga cccgatgaga cggccctgac
     6421 gatccgggac ggctggctcc acactgggga catcgcccgg gtggacgagg agggctacac
     6481 caccatcgtc gggcgcaaga aggagatgat catcgtgcgg ggcttcaagg tgttccccga
     6541 cgaggtggac ggcatcctgg cacgacaccc ggcagtgctc gaatcggcga ccatcgggat
     6601 ccccgacgcc cagagcgggg agcacgtccg ggcggtcgtc gtcctgaggc cggggcacat
     6661 tgccacgagc gaggacctga ttgcgcactg ccgcaccgag ctcgcgccct tcaaagtgcc
     6721 caaagagatc gaattccgcg cggagcttcc caagagcgcg gcgttcaagg tgctgcggcg
     6781 cgagctgcgc gccgaggcca tcgccgcgct tacaggcgca tccggcgcag cagctcggtg
     6841 aagcgcgggt ctccccgcag gccgtcgagg gtgggctcga ccttgagata ggcgagccac
     6901 ccgcggcgtt cctcgtaggc gcgttccagc caggcgaata cctcgtcggc catgccgagc
     6961 ccggcgtaga ccatgccgat cgcgaccggg gacacgtacc cggtcttgcg gtgcgcttcg
     7021 agctcctcca cgatgcgctc cgccgccgcg cgatcgccgc gcacggcgta gaggtagccg
     7081 agggcccccg tggcatacgc ggtctcgggc gagagcgaca ccgcttcgcg cagcgcgcgc
     7141 tcggcctcgg cgtactcgcc gcgctgcgtg tgcacccgtc ccagc
//
LOCUS       DBNGZW010000048         2424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1436319, whole genome shotgun sequence.
ACCESSION   DBNGZW010000048 DBNGZW010000000
VERSION     DBNGZW010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1436319"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..520)
                     /locus_tag="AC16JC_01280"
     CDS             complement(<1..520)
                     /locus_tag="AC16JC_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163358.1"
                     /translation="MSYQRFLVIYGRFLAAMGAVIPAGVLLADRRWTQQWIEILALLA
                     ACIVLRGQTVRLSKFSYLNQTALVSLAGSLLVGLPATLLALSGGTLLADWVWHRKTVW
                     AAVVNYGREAAALTASYGIYAGILSISGVADPGLYVTTIPAAFFLALAYFLFSRLLFY
                     LSLLVRGKLEESE"
     gene            complement(517..1311)
                     /gene="trpA"
                     /locus_tag="AC16JC_01285"
     CDS             complement(517..1311)
                     /gene="trpA"
                     /locus_tag="AC16JC_01285"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019461374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR5163359.1"
                     /translation="MSERPIGRAFGAARAQGRAALIPYFTAGYPSTQVCRDALRAAER
                     SGADIVEVGIPFSDPLADGPTIQRSTHAALEQGMTVAGVLELVRTVGLGVPVVLMTYL
                     NPVLAYGVERFLTDAAAAGVAGLLCTDLPGGADPAFDRAVAASPLDLIRLAAPTTSDE
                     RLAGALAGGGGFVYFISRLGVTGARPDAPPDLERHIRRLRAATRLPVAVGFGIGTPAQ
                     AAQAARWADGVVVGSAVVEALGSGGLPGVERLLDGLVRALRRRGAA"
     gene            complement(1304..>2424)
                     /locus_tag="AC16JC_01290"
     CDS             complement(1304..>2424)
                     /locus_tag="AC16JC_01290"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR5163360.1"
                     /translation="DRREMQILVSLLLAYAAREASPDKVHQISGKLTEAGATHIMVEP
                     PIETDEHLADSERAKEAAKRTYARSVAVTREVINSLRLGRTANVKRVKRAVQAIVDQV
                     LNNESSLLGLTTLRDYDEYTFVHSVNVCIFAVALGRKLGLTKLQLYDLGMTALFHDVG
                     KSRVPLDVLNKEGGLSEDEWRILQAHPWLGVLTLFGLRGYGEIPYRGMIVAFEHHMKT
                     DLTGYPKSIRPRTPSIFSKIVAVADGFDAATSRRVYQTVPIQPDQVLKEMWENPRRGY
                     DPVVVKAFINLIGVYPVGTCVILDTYEVALVHSANPDTTFIHRPVVRIVATADGAIQR
                     PGVLADLAERDAQGNFPRTIIKVTDPQKYGINVSDYFV"
BASE COUNT          395 a          854 c          832 g          343 t
ORIGIN      
        1 gctcgctctc ctcgagcttg ccgcgcacca gcagactcag gtagaacagc agccggctga
       61 acaggaagta cgcgagcgcg agaaagaacg ccgccggaat cgtggtcacg tataggccgg
      121 gatccgcgac tccggagatc gagaggatcc cggcgtagat gccgtaggac gcggtcaggg
      181 cggccgcctc ccgtccgtag ttgaccaccg ccgcccagac cgtcttgcgg tgccacaccc
      241 agtccgccag cagggtgccg ccagacagcg cgagcagcgt cgccggaagg ccgaccagca
      301 gactgcctgc gaggctcacc agagcggtct gattgaggta ggagaacttg gataaccgca
      361 ccgtctggcc gcgcagcacg atgcaggccg cgagcagtgc cagaatctcg atccactgct
      421 gggtccagcg tcggtctgcg agcaacacgc cggccgggat cacggcgccc atcgccgcga
      481 ggaagcggcc gtagatcacg aggaagcgct ggtagctcat gccgccccac gccggcgcag
      541 cgcgcgcacc agcccatcca gcagccgctc cacgcccggc agccccccgc tccccagcgc
      601 ctccaccacc gcgctgccca ccacgacgcc gtccgcccag cgggccgcct gcgccgcctg
      661 cgccggggtg ccgataccga acccgaccgc caccggcagc cgcgtcgcgg cgcgcagccg
      721 gcgtatgtga cgctcgaggt cgggcggcgc atccggccgc gcgccggtca ccccgaggcg
      781 cgagatgaag tagacgaacc cgccgccgcc ggcgagggcg ccggccagcc gctcgtcgga
      841 cgtcgtggga gccgcgagcc ggatgagatc gagcggactc gccgccacgg cgcgatcgaa
      901 cgccggatcc gccccgcccg gcaagtccgt gcagagcaag ccggctaccc ccgcggccgc
      961 cgcgtccgtg aggaaccgtt ccacaccgta ggcgagcacc ggattgagat aggtcatcag
     1021 caccacgggg acgccgagcc ccaccgtgcg caccagctcg agcacccccg cgacggtcat
     1081 gccctgctcg agggccgcgt gcgtggagcg ctgaatggtg gggccgtccg cgagcggatc
     1141 gctgaacggg atgccgactt ctacgatgtc ggcgcccgat cgctcggccg cccgcagcgc
     1201 gtcgcgacag acctgggtcg agggatagcc ggcggtgaag taggggatca atgccgcccg
     1261 cccctgcgcg cgtgccgcgc cgaacgcccg gccgatcggc cgctcagaca aagtagtcgc
     1321 tgacgttgat gccgtacttc tggggatcgg tgactttgat gatggtgcgc gggaagttgc
     1381 cctgcgcgtc gcgctcggcc aggtcggcga gtacgccggg gcgctggatc gcgccgtcgg
     1441 ccgtcgccac gatgcgcacc accggccggt gaatgaaggt cgtgtccgga ttggccgagt
     1501 gcaccagcgc cacttcgtag gtgtcgagga tgacgcacgt gccgacgggg tagacgccga
     1561 tgaggttgat gaacgccttg accaccaccg ggtcgtagcc gcggcgcggg ttctcccaca
     1621 tctccttcaa cacctggtcg ggctggatcg gcacggtctg gtagacccgc cggctcgtgg
     1681 ccgcatcgaa gccgtccgcg accgccacga tcttcgagaa gatggacggg gtccgcgggc
     1741 ggatcgactt ggggtagccc gtgagatcgg tcttcatgtg gtgctcgaag gccacgatca
     1801 tgccccggta ggggatctcg ccgtagcccc gcaggccgaa cagggtgagc acgcccagcc
     1861 agggatgggc ctgcaggatg cgccactcgt cctcgctcag gccgccttcc ttgttgagca
     1921 cgtcgagcgg cacccgggac ttgcccacgt cgtgaaacag cgccgtcatg ccgaggtcgt
     1981 agagctgcag cttggtgagg ccgagcttgc gaccgagggc gacggcgaag atgcagacgt
     2041 tcacggaatg gacgaacgtg tactcgtcgt agtcgcgcag cgtcgtgaga ccgagcagcg
     2101 acgactcatt gttgagcacc tggtcgacga tcgcctgcac cgcccgcttg acgcgcttca
     2161 cgttcgcggt ccggccgagg cgcaggctgt tgatcacctc gcgcgtcacg gcgaccgacc
     2221 gggcgtaggt tcgcttggcc gcctctttcg cccgctccga atccgcgagg tgctcgtcgg
     2281 tctcgatcgg cggctcgacc atgatgtggg tcgctcccgc ctccgtcagc ttgccggaga
     2341 tctggtgcac cttgtcgggg ctcgcctccc gcgccgcgta cgcgagcaac agcgacacga
     2401 ggatctgcat ctcccggcga tcca
//
LOCUS       DBNGZW010000049         7055 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1095560, whole genome shotgun sequence.
ACCESSION   DBNGZW010000049 DBNGZW010000000
VERSION     DBNGZW010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7055)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7055)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7055
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1095560"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..1456
                     /gene="thiC"
                     /locus_tag="AC16JC_01295"
     CDS             128..1456
                     /gene="thiC"
                     /locus_tag="AC16JC_01295"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012236883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HIR5163361.1"
                     /translation="MTCNTQMYLARQGMITDQMKRVAEREELEPEVVRHEVARGRLVI
                     PANVHHLAKRLDPMGIGKVARVKINANIGNSAVTSDIAGELEKLHHAVHYGADTVMDL
                     STGGDIDAIRQAIVDASPVPIGTVPIYQAVTETGKVEDLSADDLLDMVEHQARQGVDY
                     MTIHAGVLREHVPHALGRVTGIVSRGGSLHAYWMTAHNAQNPFYTHFDRVLEIARTYD
                     VTLSLGDGLRPGSLADANDRAQFAELEVLGELTEKAWARDVQVMIEGPGHIPMHLIEV
                     NVTKQKAICHEAPFYTLGPLVTDIAPGYDHITSAIGAAMIGWYGADMLCYVTRKEHLG
                     LPNAEEVREGVIAYKIAAHAADIARGRHGARDRDDALSRARFAFDWSEQFRLSLDPDR
                     ARELHDEALPAEYFKSAEFCAMCGPQFCSLHITREIERHFGAQEPVVAER"
     gene            complement(1464..2555)
                     /locus_tag="AC16JC_01300"
     CDS             complement(1464..2555)
                     /locus_tag="AC16JC_01300"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5163362.1"
                     /translation="MRCFFVLLALLSVATLVRAQRPPRPPSLSVLETRAAQDSLDPDA
                     QFRLALGYARKKRWDDEGRALRGAIAINPRYTPAYVALAFLPFQRRPALAREVQQRKV
                     PPMWRDSLVQMSRLLHRAFLIDPLAELEPPDVDTQREGAALAYMNLALALRFGGRPLD
                     SVPSWVLWYRGLTDGRAGRHASAIENLQALLRRGEAIESDSVTPFPVSTNEYRYLLAV
                     LCDRAGQRAEALRYYQQTLAGDLGNYMAHARLARLYVGEQLWTDAVTEAERAVAANPE
                     DPATVRELGQILLAAGRLPEAEAAVREAREKNPRDLGTEYVLGVILVRSGRTAEARVA
                     LERFLALAPPTLYGPQIDDTRQRLAALSR"
     gene            2755..3942
                     /locus_tag="AC16JC_01305"
     CDS             2755..3942
                     /locus_tag="AC16JC_01305"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018052607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HIR5163363.1"
                     /translation="MLKQTPFHPRTAALMEGQAWRRWAGYVVASSYELNHEREYHSVR
                     SAAALFDVSPLYKYLIRGPDAARLLDRIVTRDVLKCKVGQVLYTPWCDARGKVIDDGT
                     ISRLEEEAFRMTAADPSFRWLHLNATGLDVAIEDVSERTAAVSLQGPNARAILELAGG
                     QPLTELRYFRLTTGKIRGVPVTISRTGYTGDLGYEVWIDAEHACAVWDALVEVGTGYG
                     ITPAGMLALDLARNEAGLLLIEVDYISAQHALIDSQTSTPYELSLGWTVALDKERFNG
                     QDALREEQALGPAWRFVGIEVDWPSLEQLYAEVGLPPKLPTTAWRASVPLYVGGVQVG
                     YATSGCCSPLLKRYIALAHLEAVYAEPGTAVEMEVTVEHRRKRAAAAVRTLPFFDPAR
                     KRA"
     gene            3944..5527
                     /locus_tag="AC16JC_01310"
     CDS             3944..5527
                     /locus_tag="AC16JC_01310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009470192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR5163364.1"
                     /translation="MPQMWDAIIIGGGHNGLVTAAYLARAGKRVLVLERRYRVGGAAV
                     SEEIYPGFKFSVFSYVVSLLRPEIIRDLDLPRHGLEILPLESTITPMDNGDYLGSWGD
                     PDKTRREIYRHSPRDAEAMVEFGRLMHYMAQAVKPILGMIPPDPTSLSPRDLLGLLRL
                     GRHFRNLGPQQFQALYKLMTMSSADYLDEWYETDVLKATKSASGIIGTFLGPRSPGTA
                     YVLLHHYMGEIDGSFRAWGFAKGGTGAISDAIAGAAHRFGAEIRTEAPVGRVLLKRGR
                     AVGVALENGDELRAKTVVSSLDPHRTFLELVGEAALPDDFLADIHRFKYRGSSGKVNL
                     ALDELPNFTCLPGVGPHLRGAISISPSIDYLEHAYDDAKYGEFSRRPYMDVIIPSMID
                     PHMAPPGKHVMSIFVQYAPYNVTGGWTDERREAFGDAVVETLSHYAPNLKRAILHRQV
                     ITPADIERITGLTEGNIFQGELALHQLFFLRPAPRWAKYRTPVRRLYQCGACTHPGGG
                     IMGASGRLAALQILGDTGR"
     gene            5524..>7055
                     /locus_tag="AC16JC_01315"
     CDS             5524..>7055
                     /locus_tag="AC16JC_01315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013739.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR5163365.1"
                     /translation="MTREVIVIGAGVNGLVAAAYLAKAGVPVLVLEARDTPGGLAGSE
                     EMAACDAGWLLPDIAQHLGLGAAGLELVVPEASVFSLLEDGGSLTLWSDPARTAQEIA
                     RFSPEDARRWPAFADYLARFARVLEAVYAVTPPQVPNTRTRDLSMLLGLARRLRRLGK
                     RDMVEFLRVTPMSVAEWLDDWFAGDALKGAVGAGGITRILQGPRSAGTAFVLLHHHVG
                     RPAGAVRAAHLVKGGPGALARALVQVAQGFGAEIRTSAPVREIVVRDGRAIGVALETG
                     EHLAARRVVSNADPRRTLCGLVPPACLDPEFVRAVHNVRFRGAVAMVRLVLGELPRFT
                     ARADDGALRGAITVAPSLDYLERAYDDAKYGGVSRHPFLEARIPTLLDPSLAPPGRHL
                     MTVQVQYAPYHLRDGGGVWDAAARERLADTVVAAFAQHAPNLRTAVLERHVMAPPDLE
                     ERFSLTEGHPYHGEMTLDQIFFMRPVAGWARYRTPIKGLFLCGAGTHPGGGLPGAPGR
                     NAA"
BASE COUNT         1076 a         2493 c         2429 g         1057 t
ORIGIN      
        1 agtcgaccat gcagggagcg gggagcacgg agcagtccgc agcggagcgc gccgggattt
       61 ccgcacgcca ccgtcctgga cagctccgtg ctccctgctc ccgtttcgtg ccaaggagcc
      121 aaatccgatg acctgcaaca cccagatgta cctcgcccgc cagggcatga tcacggacca
      181 gatgaagcgg gtggccgagc gcgaagagct ggagcccgag gtggtgcgcc acgaggtcgc
      241 ccgcggccgg ctcgtgattc ccgccaatgt ccatcacctc gccaagcggc tcgacccgat
      301 gggcatcggg aaggtggcgc gggtgaagat caacgccaac atcgggaact ccgccgtgac
      361 gtccgacatc gcgggggagc tcgagaagct gcatcacgcc gtccactacg gggccgatac
      421 cgtgatggac ctctccaccg gcggggacat cgacgcgatc cgccaggcga tcgtcgacgc
      481 gtcgcccgtg cccatcggca cggtgccgat ctaccaggcg gtgacggaga ccggcaaggt
      541 cgaggacctg tccgccgacg acctgctcga catggtcgag caccaggcgc ggcagggcgt
      601 cgactacatg acgatccatg ccggcgtgct gcgcgagcac gtgccccacg cgctcggtcg
      661 ggtgacgggg atcgtgtcgc ggggcggctc gctccacgcc tactggatga cggcgcacaa
      721 tgcgcagaac ccgttctaca cgcacttcga ccgggtgctg gaaatcgctc ggacgtacga
      781 cgtcacgctg tcgttgggcg acgggttgcg gccgggctcg ctcgccgacg ccaacgaccg
      841 cgcgcagttc gccgagctcg aggtgctggg cgagctgacc gaaaaggcgt gggcccggga
      901 cgtccaggtg atgatcgaag gcccggggca catcccgatg catctgatcg aggtgaacgt
      961 caccaagcag aaggcgatct gtcacgaggc ccccttctac accctggggc cgctggtgac
     1021 cgacatcgcc cccggctacg accacatcac gtccgccatc ggcgcggcga tgatcggctg
     1081 gtacggcgcg gacatgctgt gttacgtgac acgcaaggaa cacctcggac tccccaatgc
     1141 cgaggaggtc cgggagggcg tgatcgccta caagatcgcg gcgcacgcgg cggatatcgc
     1201 gcgcggccgc catggtgcgc gcgaccgcga cgacgcgctg tcgcgtgccc gcttcgcatt
     1261 cgactggagc gagcagttcc gcctgagcct cgatcccgac cgggcgcgtg agctgcacga
     1321 cgaggcgctg ccggccgagt acttcaagtc ggcggagttc tgcgccatgt gcgggccgca
     1381 gttctgttcg ctgcacatca cgcgggaaat cgagcggcac ttcggggccc aggagccggt
     1441 ggtggccgag cgatgaccgg ccgctagcgc gagagggcgg ccagacgctg tctggtgtcg
     1501 tcgatctgcg gcccgtacag cgtgggcggc gcgagggcca ggaaccgctc cagcgcgacc
     1561 cgcgcctccg cggtccgccc cgatcgcacg agtatcaccc cgaggacgta ttcggtcccg
     1621 aggtcgcggg ggttcttctc cctggcttcg cgcaccgccg cctcggcctc gggtagacgt
     1681 ccggcggcca gcaggatctg cccgagctca cgcaccgtcg ccgggtcctc cgggttcgcg
     1741 gcgacggcgc gctccgcctc cgtcacggcg tccgtccaca gctgttcgcc gacgtacagg
     1801 cgggccagac gggcgtgcgc catgtaattc cccagatccc ccgcgagcgt ctgctggtaa
     1861 tagcgcaagg cctcagcgcg ctgaccggcg cggtcacaca ggactgcgag caagtagcga
     1921 tactcattgg tcgagacggg aaacggggtg acggagtcac tctcgatcgc ctccccgcgc
     1981 cgcagcagcg cctgaaggtt ctcgatcgcg gaggcatgtc gccccgctcg accatcggtc
     2041 agtccccggt accacagcac ccaggaagga accgagtcca gtggccgtcc gccgaaccgt
     2101 aatgccaggg cgagattcat gtatgccagg gcggctccct cccgttgtgt atcgacgtcc
     2161 gggggctcca actccgcgag cggatcgatg agaaaggccc ggtgcaggag gcggctcatc
     2221 tgcaccaggg agtcacgcca catcggtggg accttccgct gctggacctc ccgagcgagg
     2281 gccgggcgcc gctggaaggg aaggaatgcg agcgccacgt acgccggcgt gtatcgagga
     2341 ttgatcgcga tggccccgcg cagcgcccgc ccctcatcgt cccaccgctt cttgcgggcg
     2401 tagccgagtg cgagtcgaaa ctgcgcgtcg gggtccagcg agtcctgcgc ggcacgcgtc
     2461 tcgagcaccg agagactcgg cggccggggc ggtcgttgcg cgcggacgag cgtcgcgacg
     2521 gacaggagtg cgaggagcac aaagaagcaa cgcatgtgcc ggtcaataat tacggtctac
     2581 gccagatctt gcacgatccc agccgactgg ttgggcgccc tcgcttgacg gcgtcccgct
     2641 caggcgttga tttgtcccgc ccgtggacgt taccgcttgc atgcccaccc ccgctgtcgc
     2701 ctcgcgcgct gtgccccccc tcgagccgct gcacgggccg acgggaggga cctcatgctg
     2761 aagcaaaccc cgttccaccc gcgcaccgcc gccctcatgg aggggcaggc gtggcgtcgc
     2821 tgggccgggt acgtggtcgc gagctcttac gagctgaatc acgagcgcga ataccactcg
     2881 gtgcgcagcg ccgccgcgct cttcgacgtg tcccccctgt acaagtacct gatccggggc
     2941 ccggatgcgg cccggctgct ggaccggatc gtgacgcgcg acgtcctcaa gtgcaaggtc
     3001 ggccaggtcc tctacacccc ctggtgcgac gcgcgtggca aggtgatcga cgacggtacc
     3061 atctcccgcc tcgaggagga ggcgttccgc atgacggccg cggacccgag cttccgctgg
     3121 ctgcatctca acgcgaccgg cctcgacgtc gccatcgaag acgtctccga gcgcaccgcg
     3181 gcggtgtcgc tccaggggcc caacgcccgg gccatcctcg agctggccgg cggccagccg
     3241 ctcacggagc tcaggtactt ccgcctcacc accgggaaga tccgcggcgt cccggtgacg
     3301 atctcccgca ccgggtacac cggcgacctg ggctacgagg tgtggatcga cgcggagcac
     3361 gcctgcgccg tgtgggacgc gctcgtcgaa gtggggacgg gctacggcat cacgccggcg
     3421 gggatgctcg cgctcgatct ggcgcggaac gaggcgggtc tcctgctcat cgaggtggac
     3481 tacatctcgg cccagcacgc cctgatcgac tcgcagacgt ccacgccgta cgagttgtcg
     3541 ctcggctgga ccgtggccct cgacaaggag cggttcaacg gacaggacgc cctccgggag
     3601 gagcaggctc tcggaccggc gtggcgattc gtgggcatcg aggtggattg gccgtcgctc
     3661 gagcaactct acgccgaggt cggattgccc cccaagctcc cgaccaccgc gtggcgcgcc
     3721 agtgtcccgc tgtacgtcgg cggcgtgcag gtggggtacg cgacgagcgg ctgctgttcg
     3781 ccgctgctca agcgctacat cgcgctggcc cacctcgagg cggtctacgc cgagccgggg
     3841 acggccgtgg agatggaggt caccgtcgag caccgtcgca agcgcgcggc ggcggcggtc
     3901 cgcacgctgc cgttcttcga tcccgcgcgc aagcgagcct gagatgcccc agatgtggga
     3961 cgcgatcatc atcggcggcg gccacaacgg gctcgtcacg gcggcgtatc tggcgcgcgc
     4021 cggcaagcgc gtgctcgtcc tcgagcgccg ctatcgcgtc ggcggggccg cggtgagcga
     4081 ggagatctac ccgggattca agttctcggt gttctcttac gtcgtcagcc tgctgcggcc
     4141 ggagatcatc cgcgacctcg atctgccccg gcacgggctc gagatcctgc cgctggagag
     4201 cacgatcacg ccgatggaca acggcgacta cctcggctcc tggggcgacc ccgacaagac
     4261 gcgccgcgag atctaccgcc actctccccg ggacgccgag gcgatggtcg agttcggtcg
     4321 gctgatgcac tacatggccc aggccgtcaa accgatcctc gggatgatcc cgcccgatcc
     4381 gacgtcgctc tcgccgcggg acctgctcgg cctgctgcga ctgggccgcc atttccggaa
     4441 cctgggccct cagcagtttc aggcactcta caagctgatg acgatgagct cggccgatta
     4501 cctggacgag tggtacgaga ccgacgttct caaggccacc aagtcggcca gcggcatcat
     4561 cgggacgttc ctgggccccc gctcgccggg cacggcctac gtcctgctgc atcactacat
     4621 gggggagatc gacggctcgt tccgggcctg ggggttcgcc aagggcggca ccggagccat
     4681 cagcgacgcg atcgccggcg ccgcgcaccg cttcggcgcg gagatccgca ccgaggcgcc
     4741 cgtcgggcgc gtgctcctga aacgcgggcg ggcggtgggc gtggcgctgg agaacggcga
     4801 cgagctgcgc gccaagaccg tcgtctccag tctcgatccg caccgcacct tcctggagct
     4861 cgtcggcgag gcggcgctgc ccgacgattt cctcgccgat atccaccggt tcaagtaccg
     4921 cgggtcttcg ggcaaggtga atctggcgct cgacgagctg cccaacttca cctgcctgcc
     4981 cggcgtggga ccacacctgc gcggcgccat ctccatcagc ccgagcatcg actacctgga
     5041 acatgcgtac gacgacgcca agtacggcga gttctcccgc cggccgtaca tggatgtcat
     5101 catcccgtcg atgatcgacc cgcacatggc gccgcccgga aaacacgtca tgtcgatctt
     5161 cgtgcagtac gcaccgtaca acgtgaccgg cgggtggacc gatgagcggc gcgaggcgtt
     5221 cggcgatgcc gtcgtggaga cgctgtcgca ttacgcgccg aacctgaagc gggccatcct
     5281 gcatcgtcag gtgatcacgc ccgccgacat cgagcgcatc accggcctca ccgaggggaa
     5341 catcttccag ggcgagctgg ctttgcatca gctcttcttt ctccgcccgg cgccgcgctg
     5401 ggccaagtac cgcacgcccg tgcgccggct gtatcagtgc ggcgcgtgca cgcacccggg
     5461 cgggggcatc atgggcgcgt ccgggcggct cgccgcgctc cagatcctcg gagacacggg
     5521 gcgatgacgc gtgaggtgat cgtgatcggc gccggcgtga acggactggt cgccgcggca
     5581 tacctcgcga aggccggcgt gcccgtcctc gtgctcgagg cgcgcgatac gccgggcggg
     5641 ctcgccggga gcgaggagat ggcggcgtgc gacgcgggct ggctccttcc cgacatcgcg
     5701 cagcacctgg ggctcggcgc cgcggggctc gagctcgtag tccccgaagc gagcgtgttc
     5761 agcctcctcg aagacggcgg ctccctcacg ctctggagcg atcccgcccg gaccgcgcag
     5821 gagatcgccc ggttctcccc cgaggacgcc aggcgctggc ccgcattcgc cgactatctc
     5881 gcccgcttcg ccagagtgct ggaggccgtc tatgccgtga ccccgccgca ggtcccgaac
     5941 acccggacga gggacctctc gatgctgctg ggactggccc ggcggctgcg ccggctgggg
     6001 aaacgcgaca tggtcgagtt cctgcgcgtg acaccgatgt cggtcgcgga atggctcgac
     6061 gattggttcg ccggtgacgc gctcaagggc gccgtcggcg cgggcgggat cacccgcatc
     6121 ctccagggtc cgcgctcggc cggcaccgcg ttcgtgctgt tgcaccatca cgtcggacgg
     6181 cccgcgggag cggtgcgtgc cgcgcatctg gtcaagggcg ggccgggcgc gctggcgcgg
     6241 gcgttggtgc aggtcgcgca gggattcggc gccgagatcc gcacgtccgc gccggtgcgt
     6301 gagatcgtgg tgcgcgatgg tcgggcgatc ggcgtcgcgc tcgagaccgg cgagcatctg
     6361 gcggcccgcc gcgtcgtctc caacgccgat ccccggcgca cgctgtgcgg gctcgtccca
     6421 ccggcgtgcc tcgaccccga attcgtgcgc gccgtccaca acgtgcggtt tcgcggcgcc
     6481 gtcgccatgg tccggctggt cctcggcgag ttgccccggt tcaccgcccg cgccgacgac
     6541 ggcgcgctgc gcggagcgat caccgtcgcc ccgagcctgg actacctgga gcgcgcctac
     6601 gacgatgcga agtacggggg cgtgtcgcgc caccccttcc tggaggcgcg catcccgaca
     6661 ctgctcgatc ccagcctcgc accccccgga cggcacctga tgaccgtgca ggtacagtac
     6721 gcaccctatc acctgcgcga cggggggggc gtgtgggacg ccgccgcccg cgagcgcctc
     6781 gccgacacgg tggtcgccgc gttcgcgcag cacgcgccga acctccgcac cgccgtgctg
     6841 gagcgccacg taatggcccc gcccgatctc gaggagcgct tttccctcac ggagggccac
     6901 ccctaccacg gtgagatgac cctcgatcag attttcttca tgcgccccgt ggccggctgg
     6961 gcccgctacc gcacgccgat caaggggctg ttcctgtgcg gcgccgggac gcacccggga
     7021 ggcggtctcc ccggcgcgcc gggacggaac gccgc
//
LOCUS       DBNGZW010000050         6969 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1573191, whole genome shotgun sequence.
ACCESSION   DBNGZW010000050 DBNGZW010000000
VERSION     DBNGZW010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6969)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6969)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6969
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1573191"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="AC16JC_01320"
     CDS             <1..1106
                     /locus_tag="AC16JC_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012844234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5163366.1"
                     /translation="AALARQLDVGAAKPYASLADLVADKDVDALWLTGPNYTRVQNME
                     EIVDSLQRGRGALAGIACEKPLARNVAEATRVVALVKGAELKTGYLENQLFAPQVERG
                     RALLWARGAALTGRPYLARAAEEHAGPHMPWFWRADLQGGGVLNDMMCHSALVVRHLL
                     TKPGEPLSTVEPVRVTGRIASLKWSRPEYAKRLTHAMGKDVDYTRHPAEDFASLTVEF
                     ETADGHLVLGEATTSWSFVGAGLRLSAELLGPEYSMSWNTLDAGLKLFFSREVKGKTG
                     EDLVEKQSAEMGLMPVVADEPAAYGYEAEDRHFVRVFLGKEPPLLTFDDGLEVVKLLM
                     TAYRSAEQGKTLDFPPPDLDGFVPAVAQGTWKP"
     gene            complement(1107..3185)
                     /locus_tag="AC16JC_01325"
     CDS             complement(1107..3185)
                     /locus_tag="AC16JC_01325"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5163367.1"
                     /translation="MTDSLLSPAVEDRWGAGPAEGADELDQLVYLSNLVGREPRLVQP
                     GGGNTSVKIGEVLLVKGSGTDLRTIDRRGFTRLSLARLATLRDMTAMGDAEMMRFMAG
                     CMTVQDGPVPSVETPLHSLLPHRVIVHTHDVATMSLTNVTDAVAERLTTELLGGAIAY
                     VPYARPGFPLAHALREQLDRIPAHAIGMVLAHHGLVVWAQDFRECYDRLVRTVRRIED
                     HLAAKRRDREVPGARSAAAPPREVRERFAEAVLPGVRGALGGKDGRVILHLDDAEPVL
                     GSLGAGRLRDLVGTGMATPEHILRAGRRPLWLELDWSAPPQRAAESVRRRIAEQRTEY
                     EAYHGRHAPAGTPPIADWAKVVLVPGLGMITAGRDQRGAATANLCYRAVLETIANAEA
                     IDHFTFLSEADVFEFEHWPLERRKVEEQAARDRASLLVPGQVVVVVGGASGIGRAAAR
                     RFVEEGAHVVVADLDQSAADRAAADIAQDFPGRARGLAADVRDDASLDRLFRETVLAF
                     GGLDCLFYTAGQPPRFAQLPDIHREDLQRQLDVHYLGAVMAIGRAAAVMRRQGSGGAI
                     VASVSKAALAPGREAVAYGGSKAALLQALRVAALELGGDGIRVNAINADQIETPLFRR
                     FVAERAASRGVTVEQQLEEYRKRNAMGVSLIPAEAVADVAVLLASERFRYTTGDILTV
                     DGGLPEAFPR"
     gene            3264..4142
                     /locus_tag="AC16JC_01330"
     CDS             3264..4142
                     /locus_tag="AC16JC_01330"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR5163368.1"
                     /translation="MTSLELRTARIEPLPGAKPRDDELDLFGLTHPGKVRQENQDHFL
                     LCTVHPLAVVHATSLPNLNRLPLRGERLATVLLVADGVGGTTAGGEASQLATEAVTRY
                     VTSTLRCYHAVGADDEQFLEALRAAALEADAAVRAEATAKSMATTLTLAIAVWPWLYV
                     MQVGHSRAYYFWDGALRQITRDQTMAQDLVDRGALPPERLAASPFSNVLSSAIGGPEP
                     TPEVSRLDIRRRGCVILLCSDGLTKHVTNAEIAEQLGAMQSAEQVCRALLDLALERGG
                     SDNITILVGRAPPSKA"
     gene            4148..5116
                     /locus_tag="AC16JC_01335"
     CDS             4148..5116
                     /locus_tag="AC16JC_01335"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR5163369.1"
                     /translation="MRGPLDAIRPIALDEVREARARIAGTIVRTPLVRLELGPDYPDI
                     RIKLENLQPINAYKLRGAANAVALLSEADRRRGVWTISAGNAGQGVAYAARAAGVPCT
                     VVAIETAPVAKLERMRALGATLVLVPFEVAWKALDERAFPGVAGTFIHPFDDHNFIAG
                     HATMGLEILEDAPDTAAVIAAIGGGGLITGVGSAIKALKPEVRIWGAEPATAAPAALS
                     FAKGSPQVFTQWTASFVDGAGGQSLFPRMWERMKPVVDGCIVVTLEQTKRAMRLLAEK
                     ARVITEGAGALPVAAAFSGTAGQGPIVAVISGGNIDLKKFSDLIGA"
     gene            5143..6822
                     /locus_tag="AC16JC_01340"
     CDS             5143..6822
                     /locus_tag="AC16JC_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007106336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HIR5163370.1"
                     /translation="MPGESYDAIVVGSGISGGWAAKELTEKGLRVLLLERGKNVEHVE
                     DYVNASKGPWEYPHRGGRTKALERAYPVLKRDYPLNEKNLDWWASDQDSPYTEVKRFD
                     WYRGYQVGGRSLTWGRQSYRWSDFDFEANARDGIAVDWPIRYKDLAPWYDHVERHAGI
                     SGSREGLPQLPDGQFQPPMALNCGESLIAGRLARLFDGSRHIIPGRTANLTESLPGRA
                     PCQYRNACWLGCPFGAYFSTQSSTLPAAMATGRLTLRPFAIVTEVLYDRDRKRASGVR
                     VLDAVTGQTTDYAARVVFLCASTLNSTWLLMRSATNVWPGGLGSSSGELGHNLMDHHF
                     RVGAQGTIEGLEDKYYRGRRPTGFYIPRYRNLFGDKREYLRGFGYQGGASRQGWSRAV
                     AELGVGGAFKDAMAQPGAWTIGATAFGEMLPNHANQVTLDRSRKDKWGLPVLAIDCAT
                     GENERLMRRDMMHDMAEMLEATGVKNVQTYDRESFPGMGIHEMGTARMGRDPKTSVLN
                     AHNQVWDALNVFVTDGSCMVSTACQNPSLTYMAITARAADFAVNEMKQRNL"
BASE COUNT         1091 a         2506 c         2416 g          956 t
ORIGIN      
        1 ccgccgccct cgcccgccag ctcgacgtcg gtgccgcgaa gccctacgcc tcgctcgccg
       61 atctcgtcgc cgacaaggac gtcgacgcgc tctggctcac cgggccgaac tacacacgcg
      121 tgcagaacat ggaggagatc gtcgattcgc tgcagcgcgg gcggggcgcg ctcgccggca
      181 tcgcgtgcga aaagccgctg gcgcgcaacg tcgccgaagc cacccgcgtc gtggccctcg
      241 tgaagggtgc cgagctgaag accggctatc tcgagaacca gctgttcgcc ccgcaggtgg
      301 agcgcggccg cgccctgctg tgggcccgcg gcgccgcgct gaccgggcgc ccatacctcg
      361 cacgcgccgc cgaagagcac gccggccctc acatgccgtg gttctggcgc gccgacctgc
      421 agggcggcgg ggtgctgaac gacatgatgt gccactcggc cctggtcgtc cgccacctgc
      481 tcaccaaacc cggagagccg ctctccaccg tcgagcccgt ccgcgtcacc ggccgcatcg
      541 cgagtctcaa gtggtccagg cccgagtacg cgaagcggct cacccacgcg atgggcaagg
      601 acgtggacta cacccggcac cccgcggaag acttcgccag cctcaccgtg gagttcgaga
      661 ccgccgacgg ccatctggtg ctgggcgagg cgacgacgtc ctggagcttc gtcggtgccg
      721 gcctgcgcct ctcggccgag ctgctgggac cggagtactc gatgtcgtgg aatacgctcg
      781 acgccggcct gaagctcttc ttcagtcgtg aggtgaaggg caagaccggc gaggacctgg
      841 tcgagaagca gagcgccgaa atgggcctca tgcccgtggt ggccgacgag cccgccgcct
      901 acggctacga agccgaagac cgccacttcg tgcgcgtgtt cctcggcaag gaaccgccgc
      961 tgctcacctt cgacgacggg ctcgaagtcg tcaagctgct gatgacggcc taccggagcg
     1021 ccgagcaggg aaagaccctg gacttccctc ccccggacct cgacggcttc gtccctgccg
     1081 tcgcgcaggg gacctggaag ccctagctag cgcgggaacg cctccggcaa tcccccgtcc
     1141 acggtgagaa tgtccccggt ggtataacgg aaccgctcgc tcgccagcaa cacggcgacg
     1201 tccgcgaccg cctcggccgg gatcagcgac acccccatcg cattgcgctt ccggtactcc
     1261 tcgagctgct gctcgaccgt gaccccccgg ctggcggcgc gctcggccac gaaccggcgg
     1321 aacagcggcg tctcgatctg gtcggcgttg atggcgttga cgcggatccc gtccccgccc
     1381 agctccaggg cggccacccg cagcgcctgc aacagggccg ccttgctgcc gccgtacgcc
     1441 accgcctcgc gacccggcgc caaggccgcc ttcgacaccg aagcgacgat ggccccgccc
     1501 gacccctgac gccgcatgac cgccgcggcc cgcccgatcg ccatcacggc gcccaagtaa
     1561 tgcacgtcga gctgccgctg cagatcttcg cggtggatgt cggggagctg ggcgaagcgc
     1621 ggcggctggc ccgccgtata gaagagacaa tccagtccgc cgaacgcgag gacggtctct
     1681 cgaaagagcc ggtcgaggct cgcgtcgtcg cggacgtccg ccgcgagtcc ccgggcgcga
     1741 ccggggaagt cctgcgcgat gtccgccgcg gcccggtcgg ccgccgactg atcgaggtcg
     1801 gccacgacga cgtgggcgcc ctcctcgacg aagcggcggg ccgcggccct cccgatcccg
     1861 ctcgcgccac ccaccacgac cacgacctgg cccggcacca gcagcgacgc gcgatcgcgg
     1921 gccgcctgct cctccacctt tcgtcgctcc agcggccagt gctcgaactc gaacacgtcg
     1981 gcttccgaaa gaaacgtgaa atggtcgatc gcctccgcgt tcgcgatggt ttcgagcacc
     2041 gcccggtagc agaggttggc cgtcgccgcc ccgcgctgat cgcgtccggc cgtgatcatg
     2101 cccagtccgg ggacgagcac gactttcgcc cagtcggcga tgggcggagt gcccgccggg
     2161 gcgtggcggc cgtgatacgc ctcgtattcc gtgcgctgct cggcgatgcg ccgccgcacc
     2221 gactcggccg cccgctgggg cggcgcggac cagtccagct cgagccacag cggccggcgg
     2281 ccggcgcgca gaatgtgctc cggggtcgcc atgccggtgc cgacgagatc ccgcaaccgc
     2341 ccggcgccga gcgaccccag caccggctcc gcgtcgtcga ggtgcaggat gacccgtccg
     2401 tctttcccgc cgagcgcccc gcgcacgccc ggcagcaccg cctccgcgaa ccgctcgcgc
     2461 acctcgcgcg gcggagccgc ggcgctccgg gcgccgggga cctcgcggtc gcggcgcttg
     2521 gccgcgaggt ggtcttcgat ccttcgcacg gtgcggacca gacggtcata acactcccgg
     2581 aagtcctggg cccacaccac caggccatga tgcgccagca ccatgccgat cgcgtgcgca
     2641 ggaatccggt ccagttgctc ccgcagcgcg tgggccagcg ggaacccggg ccgcgcgtac
     2701 ggcacgtagg cgatcgctcc cccgagcagc tccgtggtga gccgctcggc cacggcgtcc
     2761 gtcacgttcg tgagactcat ggtcgcgacg tcgtgggtgt gcacgatcac ccggtgcggc
     2821 aggagcgaat gcagcggggt ctcgacgctc gggaccgggc cgtcctggac cgtcatgcag
     2881 cccgccatga aacgcatcat ctccgcgtcc cccatcgccg tcatgtcccg cagcgtggcg
     2941 agtcgcgcga gcgagaggcg ggtgaatccg cggcgatcga tggtccggag atcggtgccg
     3001 cttcccttga ccagcagcac ctccccgatc ttcacggagg tattgccccc ccccggctgc
     3061 accagcctgg gttcccgtcc gacgaggttg gagagataga cgagctgatc gagctcgtcg
     3121 gcaccttcag ccgggccggc gccccagcga tcttcgacgg ccggggagag cagggagtcg
     3181 gtcatggggc cctaagatga cgccttgcgg ccgcccggag aagctggcat gctgagtcgg
     3241 gcagcatggg gaggaaccac gcgttgacca gtctcgaatt gcggaccgct cgtatcgagc
     3301 ccctgcccgg cgcgaagccg agggacgacg agctcgacct gttcggcctc acgcacccgg
     3361 gcaaggtgcg ccaggagaac caggatcatt tcctgctctg cacggtgcac ccgctggcgg
     3421 tggtccacgc cacgagcctg cccaacctga acaggctgcc gctgcgcggc gagcggctcg
     3481 cgacggtcct cctcgtggcc gatggggtgg gcgggaccac ggccggcggc gaggccagtc
     3541 agctcgccac ggaagcggtc acgcgctacg tcacctcgac gctgcgctgc taccacgcgg
     3601 tgggcgccga cgacgagcag ttcctcgagg cgctgcgcgc cgccgccctg gaggcggacg
     3661 ccgcggtgcg cgccgaagcg acggcgaaga gcatggcgac gacgctcacc ctggccatcg
     3721 cggtatggcc ctggctgtac gtgatgcagg tggggcacag ccgcgcctac tacttctggg
     3781 acggcgcgct gcggcagatc acgcgcgatc agacgatggc gcaggacctg gtggaccgcg
     3841 gggcgctgcc gcccgagcgc ctggccgcgt cgccgttcag caacgtgctg tcgagcgcca
     3901 tcggagggcc ggaaccgacg cccgaggtga gccggctcga catccggcgg cgcgggtgcg
     3961 tcatcctgct ctgcagcgac gggctgacga agcacgtcac gaatgcggag atcgccgagc
     4021 agctcggcgc catgcaatcg gccgagcagg tgtgtcgcgc cctgctggac ctggcgctgg
     4081 agcgcggcgg cagtgacaac atcacgatcc tggtgggccg ggcgccgccg agcaaggcgt
     4141 agcacggatg cgcgggccgc tcgacgccat ccgccccatc gcactcgatg aggtccggga
     4201 agcacgcgcg cgcatcgcgg gaaccatcgt gcgcacgccg ctggtgcgcc tcgagctggg
     4261 tccggactat cccgacatcc ggatcaagct cgagaacctg cagccgatca acgcctacaa
     4321 gctgcggggc gccgccaacg ccgtcgccct gctgtccgag gccgaccgcc ggcgcggggt
     4381 ctggaccatc agcgcgggga atgcgggtca gggcgtcgcc tacgcggcgc gcgcggcggg
     4441 tgttccctgc accgtggtcg cgatcgagac cgcaccggtc gcgaaactcg agcgcatgcg
     4501 ggcgctcggc gccacgctcg tcctcgtgcc gttcgaggtc gcgtggaagg cgctggacga
     4561 gcgcgcgttc ccgggagtcg cgggcacgtt catccacccg ttcgacgacc acaacttcat
     4621 cgccggccac gcgaccatgg gactggagat cctggaagac gcgcccgaca ccgccgcggt
     4681 catcgccgcc atcggcggcg gcgggctcat cacgggcgtc ggcagcgcga tcaaggcgct
     4741 caagccggag gttcgcattt ggggcgccga gccggcgacg gccgctcccg cggcgctctc
     4801 cttcgccaag ggatcgccgc aggtgttcac gcagtggacc gcgtcgttcg tggacggcgc
     4861 cgggggccag agcctgttcc cgcggatgtg ggaacgcatg aaaccggtgg tcgatggctg
     4921 catcgtagtg acgctggagc agacgaagcg ggccatgcgg ctgctggccg agaaggcccg
     4981 tgtcattacg gagggcgcgg gggccctgcc cgtcgccgcc gccttctccg gaaccgccgg
     5041 ccagggcccg atcgtggcgg tcatctccgg cggcaacatc gacctgaaga aattcagcga
     5101 cctgatcggc gcgtgagcgc cgcgtcacaa cgggagggga atgtgccagg cgagagctat
     5161 gacgccatcg tcgtcggttc cgggatctcg gggggctggg cggcgaagga gctcaccgaa
     5221 aaggggctcc gcgtgctgct gctcgagcgg ggcaagaacg tcgagcacgt cgaggactac
     5281 gtgaacgcgt ccaagggccc ctgggagtac ccccaccgcg gcgggcggac gaaggcgctg
     5341 gaacgcgcct acccggtgct caagcgggat tacccgctga atgagaagaa cctggactgg
     5401 tgggcctcgg atcaggactc gccctacacc gaggtgaagc ggttcgactg gtaccgcggc
     5461 taccaggtgg gcggccgctc cctcacgtgg ggacgccaga gctaccgctg gagcgacttc
     5521 gatttcgagg ccaacgcgcg ggacggcatc gccgtcgact ggccgatccg ctacaaggac
     5581 ctcgccccct ggtacgacca cgtcgagcgc cacgccggga tctcgggctc ccgcgagggg
     5641 ctgccgcagc tgcccgatgg tcagtttcag ccgcccatgg cgctcaactg cggcgagtcg
     5701 ctgatcgccg ggcggctcgc gcgcctgttc gacgggagtc gacacatcat cccgggacgg
     5761 accgcgaacc tcacggagtc cctccccggc cgcgcgccgt gccagtaccg caacgcctgc
     5821 tggctggggt gcccgttcgg ggcgtacttc agcacgcagt cctccaccct ccccgccgcc
     5881 atggccaccg gccggctgac cctccgcccg ttcgccatcg tcacggaggt gctctacgac
     5941 cgtgaccgca agcgcgcgtc cggcgtgcgc gtcctcgacg ccgtcaccgg gcagactacc
     6001 gactacgcag cgcgcgtcgt cttcctctgc gcctcgacgc tgaactcgac gtggctcctc
     6061 atgcgctccg cgacgaatgt gtggccgggg ggcctgggca gcagcagcgg cgagctgggc
     6121 cacaacctga tggaccacca cttccgcgtc ggtgcgcagg gcacgatcga gggactcgag
     6181 gacaagtact accgcggccg gcggcccacg gggttctaca tcccgcgcta tcgcaacctg
     6241 ttcggggaca agcgcgagta tctgcgcggg ttcgggtatc agggcggcgc gagccggcag
     6301 gggtggtccc gcgcggtcgc cgagctgggg gtgggcgggg cgttcaagga cgcgatggcg
     6361 caaccgggag cgtggacgat cggggccacg gcgttcggcg aaatgctgcc gaaccacgcg
     6421 aaccaggtca cgctcgaccg gtcccgaaag gacaaatggg ggctgcccgt gctggcgatc
     6481 gactgcgcca cgggggagaa cgagcgcctg atgcgccggg acatgatgca cgacatggcg
     6541 gagatgctcg aggccaccgg cgtcaagaac gtgcagacgt acgaccgcga gtcgttcccc
     6601 gggatgggca tccacgagat ggggaccgcg cgcatgggcc gggatccgaa gacctcggtc
     6661 ctcaacgcgc acaaccaggt atgggacgcg ctcaacgtat tcgtcaccga cggctcgtgc
     6721 atggtgtcca ccgcctgcca gaatccgtcg ctcacctaca tggcgatcac ggcgcgggcc
     6781 gcggattttg ccgtgaacga gatgaaacag cgcaacctct agaccgattt ccacggagtc
     6841 gaccatgcca tcggaaatga aaccggaccg actgatcgac cgccgcgagg cgatccggcg
     6901 cgtgagcgcc ctgctcggcg gcatcgccct ggtcggcgaa ccccacctcc ttgcgggcac
     6961 ccgccccac
//
LOCUS       DBNGZW010000051         4066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1366513, whole genome shotgun sequence.
ACCESSION   DBNGZW010000051 DBNGZW010000000
VERSION     DBNGZW010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1366513"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..406
                     /locus_tag="AC16JC_01345"
     CDS             <1..406
                     /locus_tag="AC16JC_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5163371.1"
                     /translation="LTQRSRPQLFSNALPPTVAASALAAVRCLEAQPERVRRLHENAR
                     YFREQLQALGFQPLPGETPIVPVILGETSKAIEMSDLLLGEGVFVTGFGYPVVPQGHA
                     RVRCQLSAAHTRDDLDFALRAFTRVGRRLKLI"
     gene            626..1264
                     /locus_tag="AC16JC_01350"
     CDS             626..1264
                     /locus_tag="AC16JC_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163372.1"
                     /translation="MKPLSRVALVALICAPLARPAAAQANGGNHSYKWYWGGQAGTLF
                     NRTNARKWHFDPLIGVHWMITRKRGALYVGGEEAFFTSDAPAAVTDFATGTGYNVVFS
                     KVRRIFAGLVTYPLPGHIEPMFGGGFGLNTVVDPRATSAAGQAAADDASNWANFFLLG
                     GVQINVGNLAVYGQYVLGTSANNKLLLQEQHSIQGGVRYALGKAREDVQSAH"
     gene            complement(1302..1886)
                     /locus_tag="AC16JC_01355"
     CDS             complement(1302..1886)
                     /locus_tag="AC16JC_01355"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008410082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIR5163373.1"
                     /translation="MIAPPPLGRAFYARPTSEVARRLLGHLLMSRIGNRIAVGRIVET
                     EAYVGPHDPACHAFGLRRTARTEALYDRPATAYVYFTYGMHWCLNVVTERVDYPAAVL
                     IRALQPLEGLEVMRQRRGGVPDRELCAGPARLCQALGITGIENRTSLIEGRVTILRGS
                     GRRPRIAVTPRIGVTQAADWPLRFVIAESPWASR"
     gene            complement(1883..3649)
                     /locus_tag="AC16JC_01360"
     CDS             complement(1883..3649)
                     /locus_tag="AC16JC_01360"
                     /inference="COORDINATES: protein motif:HMM:NF014045.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR5163374.1"
                     /translation="MIDFDNVTMGIRSDLQSELKRLLQSDIIRGKVAVQRAYADWRRY
                     PQYIVPLSESSIDLIFAPAYGSSKKNATDIRLAIDALELVFTRPEIGTFILLSGDSDF
                     SSLVLKLKEYGKYVIGVGIRESSSDLLIQNCDEYFSYNELAGLTKESDIEHVRRDPWE
                     LVIEAVKQMTAHNDVMRSDRLKQVMQEIDPSFDEKDVGFSRFSKFVQEAAHRGLIATT
                     RLENGQYEIALGQKAEGAAATPGPRGEAPAAAETPPPAPPAPPAPPAVPHRRGSLVGD
                     AFGLLKQALGRLGGEQEVTVEAVREAMNELAPEGTRRVDSRSMPKLLRQAHDADLIDL
                     SKDESGSYSVRVKPEEQAEPPRAPVARPQEEEAEEPEPPMPASSAPPPPYSQPTSPFG
                     GMPARSARFRRGSRGPRPAAPGAPRVGIVEVDPNFRPRVDPVSPRPAPPPQGGGEPSG
                     SGRDEDERPRRERGGRGRGGRGRGARGRNGEGGGEAGGGERSARGRRGGRGRGGRGGG
                     RSEERGPERGVQDRSGQDRGGQERGDRGEPYRQTPPTAFEAAPPPPAPERSAPIPPPA
                     PSQKDEGDSFWSRVKRGLIGGE"
BASE COUNT          595 a         1442 c         1371 g          658 t
ORIGIN      
        1 cctcacccag cgctccaggc cccagctgtt cagcaacgcc ctgcccccga ccgtggcggc
       61 cagcgcgctg gctgccgtcc gctgcctcga ggcccagccc gagcgtgtgc gccggctgca
      121 cgagaacgcc cggtacttcc gcgagcagct gcaggcgctg ggcttccagc cgctaccggg
      181 cgagacgccg atcgtgccgg tgattctcgg ggagacttcg aaggcgatcg agatgagcga
      241 cctgctgctc ggggagggtg tgttcgtgac cgggttcggc tacccggtgg tgccgcaggg
      301 gcacgcgcgg gtgcgatgcc agctctccgc ggcgcacacc cgtgacgacc tcgacttcgc
      361 gttgcgcgcg ttcacgagag tgggaaggcg cctcaagctg atttaagaca tcatcaagcc
      421 atctttaaag cacaagcagc agttatttgc gggatatctt cgggttttcg cggccgacct
      481 caagtagttt cacgttgcaa gcagataacc gacactaccg caatagacta gcttcgaacc
      541 caccgcttgc caaactgccg aggactccta ttttgggggt ccacccggca gccccgcgct
      601 gcccgaccat ccgtggagga gtccgatgaa gccgttgtcc cgtgtggcgc ttgtcgccct
      661 gatatgtgct cccctggccc ggccggccgc cgcacaggcg aacggaggaa atcattccta
      721 caagtggtac tggggcggcc aggccggcac gctcttcaac cggaccaacg cccggaagtg
      781 gcacttcgac cccctcatcg gggtgcactg gatgatcacc aggaagcgtg gcgcgctcta
      841 tgtcggtggg gaggaggcct tcttcacgag cgacgcgccg gccgccgtga ccgatttcgc
      901 gacgggcacc ggctacaacg tcgtgttcag taaggtccgg cgcatcttcg ccggcctcgt
      961 cacctacccc ttgcccggtc acatcgaacc gatgttcggg ggaggtttcg gcctcaatac
     1021 cgtcgtggac ccgagagcca cttcggctgc cggccaggcc gcggccgatg atgcgagtaa
     1081 ctgggccaat ttcttcctgt tggggggggt gcagatcaac gtcgggaacc tggccgtgta
     1141 cggccagtac gtcctcggga cctcggccaa caacaagctg ctgctacagg aacagcattc
     1201 gatccagggc ggcgtgcgct atgccctggg caaggcccgg gaagacgtcc agtctgcgca
     1261 ctgagggggc gaacggagcc gggtgacccc cggctcctgc ttcagcggga cgcccagggc
     1321 gattccgcaa tcacgaaccg caggggccag tccgccgcct gcgtgacccc gatgcggggg
     1381 gtgacggcga tgcggggccg ccggccactc ccccgcagga tcgtcaccct cccctcgatc
     1441 aacgacgtac ggttctcaat ccccgtgatg ccgagcgcct ggcaaagacg ggccgggccc
     1501 gcacacagct cccggtcggg cacgcctccc cggcgctgcc gcatcacctc gagtccctcc
     1561 agcggctgca gcgcgcgaat cagcaccgcg gcggggtaat cgacccgctc ggtgacgacg
     1621 ttcagacacc agtgcatgcc gtaggtgaaa tagacgtagg cggtggcggg gcggtcgtag
     1681 agtgcctcgg tccgggccgt gcgtcggagc ccgaacgcgt gacaggcggg atcatggggc
     1741 ccaacgtagg cctcggtctc gacgatccgg cccacggcga tccggttccc gatccggctc
     1801 atcaggaggt gccccaacag ccgacgcgcg acctcggacg tcggtcgcgc gtagaaggcc
     1861 ctgccgagcg gcggcggtgc gatcactcgc cgccgatgag tccccgcttc acccgtgacc
     1921 agaacgagtc tccctcgtcc ttctgggatg gagcgggcgg cggaatcggt gcagaccgct
     1981 ccggcgccgg cggcggtggc gcggcctcga aggccgttgg cggcgtttga cgatagggtt
     2041 cgcctcgatc gccccgctcc tgaccgcccc ggtcctggcc gctgcggtct tgaacgccgc
     2101 gctcaggccc gcgttcctcg ctgcgcccgc ccccccggcc cccccgtccc cggccgcccc
     2161 gacgcccacg cgccgaacgc tcgcccccgc ccgcctcccc accaccctcg ccattccgtc
     2221 cgcgagcccc acgaccacgc ccgccgcggc cgcgcccgcc gcgctcgcgt cgcggccgct
     2281 cgtcctcatc gcggcctgag ccgctgggct caccgccccc ctgtgggggg ggcgcgggcc
     2341 ggggcgacac aggatccacg cggggccgga aattggggtc tacctctacg atcccgaccc
     2401 gcggagcacc cggtgcagcg ggccgcggcc cgcgtgagcc gcgccggaat ctggcactcc
     2461 gggcgggcat ccccccgaac ggggacgtcg gctgggaata gggcggcggt ggcgcagacg
     2521 aggcgggcat cggcggctcg ggctcctccg cctcctcctc ctgggggcgc gccaccggag
     2581 cgcgcggcgg ctcggcctgc tcctccggct tcacccggac cgagtacgaa ccgctctcgt
     2641 ccttggagag atcgatcaga tcggcgtcgt gcgcctggcg caacagcttc ggcatgctgc
     2701 gcgagtccac gcgcctggtc ccctccggcg cgagctcatt catcgcttcc cgcactgcct
     2761 cgacagtcac ctcctgctct cctcccaatc gcccgagcgc ctgcttgagc aacccgaacg
     2821 cgtcgcccac cagcgagccg cgacggtgcg gcacggcggg cggagcgggc ggggcgggcg
     2881 gggcgggcgg tggagtctcg gcggccgccg gcgcctcgcc gcgtgggccg ggtgtcgcgg
     2941 cggcgccctc ggctttctgg cccagcgcga tctcgtattg gccgttctcg aggcgggtcg
     3001 tggcgatcag cccgcgatgg gcggcctcct ggacgaactt gctgaatcgc gagaatccga
     3061 cgtccttctc gtcgaacgag ggatcgatct cctgcatcac ctgcttcagc cggtcggagc
     3121 gcatcacgtc gttgtgcgcc gtcatctgtt tcaccgcctc gatcacgagc tcccacgggt
     3181 cgcggcgcac gtgctcgatg tcgctctcct tggtgaggcc ggcgagctcg ttgtacgaga
     3241 agtactcgtc gcagttctgg atcaggagat cgctcgaaga ctcccggatc ccgacgccga
     3301 tgacgtactt gccgtactcc ttgagcttca gcacgagcga ggagaaatcg ctgtcccccg
     3361 acaggaggat gaacgtgccg atttccggcc gggtgaagac cagctcgagc gcgtcgatgg
     3421 cgagccggat gtcggtcgcg ttcttcttgc tggagccgta ggccggcgcg aagatgagat
     3481 cgatggagga ttccgacagc ggaacgatgt actgcgggta gcggcgccag tcggcgtacg
     3541 cgcgctggac cgccaccttg ccgcggatga tgtccgactg cagcaaccgt ttcagctcgc
     3601 tctgcagatc cgagcggatg cccatcgtca cgttgtcgaa atcgatcatc agcgcggcgt
     3661 tgggggcgtg agtgggtggt tccttgaacg ctggtctggg aaggtgggcg tgtgcctgtg
     3721 tggccattgt atgggtgtgg tgccggggac cgccgccgcg cctcctgggc cgcgcttccc
     3781 gcccccgatc ggcggaaagg ctcctcgcgc tgcgactatc cctcgagatc ctgcgcggca
     3841 gccccatcgc gcaccgcgcg tctggcgagc tgtccgcttc gcacgcccca ccggtacgtc
     3901 tcgggcgacc tgggtcgggt ccgggcctgt cggcccggca cggcgaaccc agcgcatcct
     3961 gagcggcggc ggggtgagcc cggaaggggc gctgaggcgg ggcgaacccc cgtcctccgc
     4021 tcctgttccg gactccatcg ggaaccattg aaattaaaca acttgc
//
LOCUS       DBNGZW010000052         3322 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1164358, whole genome shotgun sequence.
ACCESSION   DBNGZW010000052 DBNGZW010000000
VERSION     DBNGZW010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1164358"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..767)
                     /locus_tag="AC16JC_01365"
     CDS             complement(54..767)
                     /locus_tag="AC16JC_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5163375.1"
                     /translation="MITGPIIAEIAALVGDPARATMMSALLDGRALTASELAFAAQIT
                     PQTASAHLAKLTEGGLLSVVRNGRHRHFRLASPTVVEMIDGIVAVALAKRPRYRPLSR
                     QARALSAARICYDHLAGRLSVDLTDSLVAREYVVPDGEAAEITPAGIRFFTEFGIELP
                     PLRTSRRHFCRLCFDWTERRPHLAGALGAATTKRFFDLGWMERMPRSHAVIVTPLGRR
                     GFRGTFGVDTSEPGDRRNR"
     gene            complement(793..1989)
                     /locus_tag="AC16JC_01370"
     CDS             complement(793..1989)
                     /locus_tag="AC16JC_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5163376.1"
                     /translation="MSPERWDEELPYEGLRVIDLSQGVAGPHCGMLFALYGADVIKVE
                     PPEGDWGRAIGKRHGDFSAYAIAFNRGKRSVALDLRRAEGREVAGRLLARADVVIENN
                     RPGVLARYELDYEHVQAVNRDVIYVSVTGFGQNGPNRDLPATDSILQAYSGLMAVNRD
                     AAGMPQRISVLVIDVVTGLYAHQAAATALYRRATRGGGRHVTVSLMDAIGAVQAGKMI
                     EYHLEGEHGEPGGVPVATFRTEDGYMTINARREPHFVALCQVLGLPELARDPRYATGA
                     ARLAHGAELMPLLRERVKTWPMRDLEAALKAADVLHSPVNDYRHYWDDAHVAATGAVA
                     WVEHPQVGRIPLHRIPGLPQPAPASSAARSPVTGEHTRDVLAEIGLDPAALAALEAAG
                     VVRAPA"
     gene            complement(1993..2841)
                     /locus_tag="AC16JC_01375"
     CDS             complement(1993..2841)
                     /locus_tag="AC16JC_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR5163377.1"
                     /translation="MPANVLLIDTPHPGVRRLTMNRPEKRNALNNELRGAILAALQEA
                     DADPDVKVSILRGAGLAFSAGYDLGANNAVDQPYYTAGGAGQWARHVVDGWFRIWDLA
                     KPVIAQVHGYCLAGGSELATSCDLVYVAEDARIGYPPTRLMSPPDNQFHPWLLGMRTA
                     MEMFLTGDAITGVEAAAKGFANRAFPAAELETAVLGIAERVALVPTELQQINKRSVHR
                     AMEITGLRAAIRSGTELQALAFTTEPSLAYRRQFKRDGGSVRDLLSQRDAPFQDYREQ
                     GKDQAD"
     gene            complement(2841..>3322)
                     /locus_tag="AC16JC_01380"
     CDS             complement(2841..>3322)
                     /locus_tag="AC16JC_01380"
                     /inference="COORDINATES: protein motif:HMM:NF024771.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HIR5163378.1"
                     /translation="EPHRIFWLEDPMPWYDERYALRRLKAEVDVPIAAGETEYTPFGL
                     RTMVTEGLVDYLIIDSTWAGGLTTWKKAAVMTELYQVPLAAHHDPQIHVHAVAASPTG
                     FILESFADPTRDPLWFELFRERPKIVDGFMTVPDAPGLGDRATTHRPSVPTTSTGGV"
BASE COUNT          490 a         1106 c         1189 g          537 t
ORIGIN      
        1 tcgacggtgg acgaagcatg gaccagcgcc cgttcgtacg gggctcggcc gatctagcgg
       61 ttcctgcggt cgcctggttc ggacgtgtcg acgccgaagg tcccccggaa gccacgccgt
      121 cccaaagggg tgacgatgac ggcgtggctg cgcggcatcc tctccatcca gccgagatcg
      181 aagaagcgct tggtggtcgc ggccccgagc gcacccgcga ggtgtgggcg tcgctcggtc
      241 caatcgaagc agagccggca aaagtgacgg cggctcgtac gcagtggggg aagctcgatc
      301 ccgaactcgg tgaagaagcg gatgccggcc ggcgtgatct cggcggcctc gccgtcgggc
      361 acgacatact cgcgggcgac gagggaatcg gtgaggtcaa cgctgaggcg accggccagg
      421 tggtcgtagc agattcgggc cgcactgagc gcgcgtgctt gacgggagag cgggcggtat
      481 cgcggtctct tggcaagagc gacggcgacg atcccgtcga tcatctcgac cacggtcggc
      541 gaggcaagcc ggaaatggcg gtgccgcccg ttacggacca ccgagagcag gcccccctcg
      601 gtcagcttcg cgagatgggc gctggcggtt tgaggcgtga tttgcgccgc gaaggcaagc
      661 tcgctcgccg tcagggcacg gccatcgaga agcgccgaca tcatggtagc tctcgccggg
      721 tctccgacga gcgccgcgat ctcggcgatg atggggcctg taatcattgt ccgcctatct
      781 tatgccgtcg gctcacgctg gggcgcgcac gacgcccgcc gcttcgagcg ctgcgagcgc
      841 ggccggatcg agcccgatct ccgccagcac gtcgcgcgtg tgctcgccgg tcacgggact
      901 gcgagctgcc gaggaggccg gggccggctg cgggagccct gggatgcggt ggaggggaat
      961 ccgccccacc tgcggatgct cgacccacgc gaccgcgccg gtggcggcca cgtgggcgtc
     1021 gtcccagtaa tgccggtagt cgttgaccgg cgaatggaga acgtcggcag ccttgagtgc
     1081 cgcctcgagg tcgcgcatcg gccacgtctt cacgcgctcc cgcaggagtg gcatcagctc
     1141 ggccccgtgc gcgaggcgcg cggcgcccgt cgcgtagcgc gggtcacggg cgagctcggg
     1201 cagcccgagc acctgacaga gagcgacgaa gtggggctcc cggcgggcgt tgatggtcat
     1261 gtaaccgtcc tctgttctga aggttgccac cggcacgcct cccggctccc cgtgctctcc
     1321 ctcgaggtgg tactcgatca tcttgccggc ctgtaccgcg ccgatcgcgt ccatcaggct
     1381 cacggtcacg tggcggccgc cgccccgcgt ggcccggcgg tagagcgccg tcgcggcggc
     1441 ctgatgcgcg tagaggccgg tgacgacgtc gatcacgagc acgctgatgc gctggggcat
     1501 gccggccgcg tcgcggttca cggccatcag gccggaatac gcctggagga tcgaatcagt
     1561 ggccggcagg tcgcggttcg gtccgttctg gccgaatcct gtcacggaga cgtagatcac
     1621 gtcacggttc accgcctgca cgtgctcgta gtccagctcg tagcgggcga gcacgcccgg
     1681 ccggttgttc tcgatcacca cgtcggcgcg agcgaggagc cgccccgcca cctcccggcc
     1741 ctccgcgcgc ctgagatcga gggccacgct gcgcttgccc cggttgaagg cgatggcgta
     1801 ggcgctgaag tctccgtggc gcttcccgat ggcgcgtccc cagtccccct ccgggggctc
     1861 caccttgatg acatcggcgc cgtagagcgc gaagagcatg ccgcagtgcg ggcccgccac
     1921 gccctgggac aggtcgatga ccctgagtcc ctcgtagggc agttcctcgt cccacctttc
     1981 ggggctcatg agctagtcgg cctggtcctt gccttgttcc cggtagtcct gaaacggcgc
     2041 gtctcgctgg ctgagcagat cgcgcacgct cccgccgtcc cgcttgaact ggcggcggta
     2101 cgcgaggcta ggctccgtcg tgaaggcgag cgcctgcagt tctgtgccgg atcggatcgc
     2161 cgcacgcagc cccgtgatct ccatcgcccg gtgcacggaa cgcttgttga tttgctgcag
     2221 ctcggtcggc acgagggcca cgcgctcggc gatgccgagc acggccgtct ccagctcggc
     2281 ggccgggaag gcgcgattgg cgaagccctt ggcggccgct tccacgccgg tgatggcgtc
     2341 acccgtcagg aacatctcca tggccgtccg catgccgagc agccagggat ggaactgatt
     2401 gtcgggaggg ctcatcaggc gcgtgggcgg atagccgatg cgcgcatcct cggcgacgta
     2461 gaccagatcg cagctcgtcg cgagctcgct gcccccggcc aggcaatacc catgaacctg
     2521 ggcgatgacc ggcttcgcga ggtcccagat cctgaaccag ccgtccacga catgccgcgc
     2581 ccactgtccg gcgccgccgg ccgtgtagta cggctggtcg acggcgttgt tcgcgccgag
     2641 atcgtagccc gcgctgaagg cgaggccggc gccgcgaagg atggagacct tcacgtcggg
     2701 gtcggcgtcg gcctcctgaa gggcggccag gatggcgccg cgcagctcgt tgttgagcgc
     2761 gttgcgcttc tccggacggt tcatcgtgag ccgccgcacg ccggggtgtg gcgtatcgat
     2821 caagagcacg ttcgcgggca tcacactcct cctgtggatg ttgtcggcac gctcgggcga
     2881 tgggtcgtcg cgcgatcccc gagtcccggg gcgtcgggca ccgtcatgaa cccgtcgaca
     2941 atcttcgggc gctcgcgaaa cagctcgaac cagagcggat cgcgcgtcgg atcggcgaag
     3001 gattcgagga tgaagccggt cggactggcg gccacggcgt ggacgtggat ctgggggtcg
     3061 tgatgggcgg cgagcggcac ctggtacagc tcggtcatga ccgcggcctt cttccaggtg
     3121 gtgaggccgc ccgcccacgt cgagtcgatg atcaggtagt cgacgagccc ttcggtcacc
     3181 atcgtgcgca ggccgaaggg cgtgtattcg gtctcgccgg ccgcgatggg gacgtcgacc
     3241 tccgccttca gacggcgcaa cgcgtagcgc tcgtcgtacc acggcattgg atcctcgagc
     3301 cagaagatgc ggtggggctc gc
//
LOCUS       DBNGZW010000053         1944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_549957, whole genome shotgun sequence.
ACCESSION   DBNGZW010000053 DBNGZW010000000
VERSION     DBNGZW010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1944
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_549957"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1561
                     /locus_tag="AC16JC_01385"
     CDS             <1..1561
                     /locus_tag="AC16JC_01385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163379.1"
                     /translation="RVRLTARGVADSVDLSGEFRGPLVYQNPSANADTVYGTWVVHLT
                     GDWREDPAVGAARRLTYFMEHGRSSKEDSEAEAEEAPSPYARLVERAQSDSSTLDTLV
                     ALRLAATDPKTRADIEAALFAARGETPGRLTSRLLKTETRIAPDLVLQEFRYPEEDSV
                     FGLEAARFLARELGPLALQRRRLTDREDLGAGVLSMLGAGYGFVREAGPVLQAAAQRA
                     DDPASRDLLWLAAYQADPARYRRSLEAGVDSVRGYGPIALAWVAGNGAPTLQSWGVEP
                     GELDSLRRFPGVDAAPDSLAAFLEQTTSAGARHLAPLRMRFESEGRDLGQELGRRFVS
                     DTTFRRREVIARYLERLSDTTAVPWLRGLLQKSARMRAVAYELLSPDTVRDTTLIADV
                     QRLLLGYVAGRAGLRDTAGKNVPAPWVHDERPDVRILASDGILPSAIEPWRRLFMVMP
                     MDSVQARVATDGLQMAWVVSPLRRIGGRFYVSVTLVPVGGPCLCGGGVQFVLERRQRE
                     WVAVDVERWIS"
     gene            complement(1636..>1944)
                     /locus_tag="AC16JC_01390"
     CDS             complement(1636..>1944)
                     /locus_tag="AC16JC_01390"
                     /inference="COORDINATES: protein motif:HMM:NF023459.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbK domain-containing protein"
                     /protein_id="HIR5163380.1"
                     /translation="GGELGFVKVRYKQPDGDRSRLLTLPVSGEPGRTPSPEFRFQTAV
                     AEFGLLLKGSAYRGVANFEEVIAAARSSLGADPDGYRAEFVKLAQAAQSLGLATTGSR
                     "
BASE COUNT          283 a          647 c          706 g          308 t
ORIGIN      
        1 gcgggtccga ctgacggctc ggggtgtggc agacagtgtc gacctttccg gtgagtttcg
       61 tgggccgctc gtgtatcaga atccgtcggc gaacgccgac accgtctatg ggacctgggt
      121 cgtgcacctg accggcgact ggcgggagga tccggctgtc ggcgccgccc gccggctcac
      181 ctatttcatg gagcacggcc gatcctccaa agaggattcc gaggctgagg ctgaagaagc
      241 gccatcgccc tatgcgaggc tggtggaacg cgcgcaatcg gactcgagca cgctcgacac
      301 gctcgtcgcg cttcgcctgg ctgcgaccga tccgaagacg cgggccgaca tcgaggcggc
      361 cctgttcgcg gcgcgaggag aaacgccggg acgactcacc agccggctgc tcaagacgga
      421 gacgcgcatc gcccccgacc tggtgctcca ggagttccga tatcccgagg aggactcggt
      481 gtttgggctg gaagcggccc ggtttctcgc gcgggagctg gggccgctcg cgctccagcg
      541 ccggcgcctg accgatcgag aggatctcgg cgccggcgtg ctgagcatgc tgggcgcggg
      601 ctatggattc gtacgcgagg cgggtccggt tctccaggcg gccgctcagc gcgccgacga
      661 tccggcgagc cgtgacctgc tgtggctcgc ggcgtaccag gccgatccgg cccgctaccg
      721 gcgatcactc gaggccggcg tggactcggt gcgaggctac ggtccgatcg ccctggcatg
      781 ggtcgcgggg aacggcgcac cgaccttgca gagctgggga gtcgagccgg gcgaactcga
      841 ttccctgcga cggttccccg gcgtggacgc cgccccagac tcgctcgcgg cgtttctcga
      901 acagaccacg agtgccggcg cgcgtcacct cgccccgctc cggatgcgct tcgagtccga
      961 gggccgcgat ctggggcagg agctcgggcg gcggttcgtg tccgacacca cgttccgccg
     1021 gcgcgaggtg atcgcccgct atctcgagcg cctgagcgat acgaccgcgg tgccgtggtt
     1081 gcggggactg ctgcagaagt ccgcgcgcat gcgcgcggtc gcctacgagc tgctttcgcc
     1141 ggacaccgtg cgggacacga cgctcatcgc ggacgtgcag cgcctgctgc tcggctacgt
     1201 cgccgggcgc gctgggctgc gggacactgc cggcaagaac gtcccggcac cctgggtgca
     1261 cgacgagcgc cccgacgtcc gcattctcgc gagcgacggg attctgccgt cggcgatcga
     1321 accatggcga cggctgttca tggttatgcc gatggactcg gtacaggcgc gggtcgcaac
     1381 cgatgggctc cagatggcgt gggtcgtgag cccgctgaga cggatcggag gccggttcta
     1441 cgtgtccgtg acgctcgtgc cggttggcgg tccgtgcctg tgcggaggcg gtgtacagtt
     1501 cgtgctcgag cggcgccagc gcgaatgggt cgcggttgac gtggaacgct ggatctcgta
     1561 gcccggggtc gttcgagcga aacgccgacc ctccggacac gcgcccggag ggccttcggt
     1621 agtatgggcg aaatctcacc gcgatccggt cgtggccagc ccgagcgatt gcgcggcctg
     1681 cgccagcttc acgaactcgg cgcgatagcc gtcgggatcg gcgccgagcg aacttcgagc
     1741 cgccgcgatc acctcttcga aattcgccac gccgcgatac gccgagccct tgagcagcag
     1801 accgaattcg gccactgccg tctggaaccg gaattcgggt gagggggtgc ggcccggctc
     1861 gcccgagacg ggaagggtga ggagccggct gcgatccccg tcgggctgct tgtagcgcac
     1921 cttgacgaag cccagctccc cgcc
//
LOCUS       DBNGZW010000054         6601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1640947, whole genome shotgun sequence.
ACCESSION   DBNGZW010000054 DBNGZW010000000
VERSION     DBNGZW010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1640947"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="AC16JC_01395"
     CDS             <1..1212
                     /locus_tag="AC16JC_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003297375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR5163381.1"
                     /translation="LAELRVLDCSRVLAGPWAGQILADLGADVIKVERPGTGDDTRHW
                     GPPYLRDQTGKETSEAAYFLCANRGKRSLTVDFATTDGRDIVRRLAAQSDVFLENHKV
                     GDLARHGLDFESLRALNPRLVYCSITGFGQSGPHAPRAGYDFAIQALGGLMSVTGERD
                     DRPGGGPQKVGVAVADVMAGLYAAIAVLAALMHRERSGLGQHIDLGLLDVQVATLANV
                     ALNYLVGGEVPRRWGNAHANIVPYQVLPTADRPIVLVVGNDEQFRKFCEVAGAPALAA
                     DARYATNEARVRHREALTRELERVLARRSAAAWLAALEPAGVPCAPINDVAEVFDDPQ
                     VRHRGLRVEVPHPLAGTVPLVANPIRLSEAPIRYDAPPPLLGQHTDEILRERLGFAPA
                     AIADLRRRGVV"
     gene            1272..1673
                     /locus_tag="AC16JC_01400"
     CDS             1272..1673
                     /locus_tag="AC16JC_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163382.1"
                     /translation="MSDGVPRQQAFLLNGFTQPPGHGSATVITYDRPARWQRALKALG
                     KWWGIALLSVFIPVAHFILVPSFFLFGLYQFSQRLGAVEVPADAHGKCPDCGAEQPLE
                     LASQWHVPQLVTCHQCHRGLRLTLPDMDTTA"
     gene            complement(1748..2035)
                     /locus_tag="AC16JC_01405"
     CDS             complement(1748..2035)
                     /locus_tag="AC16JC_01405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006567569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR5163383.1"
                     /translation="MNKAELIAALAMRTKMSKADSGRAVEALFDDKGIIAGELKKGAK
                     VQITGFGNFEARKRAARVGRNPKTGAEIQIKATVAPAFRAGKQLKMAVNKK"
     gene            2349..2954
                     /locus_tag="AC16JC_01410"
     CDS             2349..2954
                     /locus_tag="AC16JC_01410"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017471056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIR5163384.1"
                     /translation="MFHGTRPGWIEVIAGVMFAGKSEELIRRVRRAAIARKQVQVFKS
                     HLDARYAGLYSVTTHDGITVEAEPVDCSAQIVRAVRLDTEVVAVDEAQFLDEGIVDVS
                     GALADRGVRVILAGTDVDFRGLPFGPMPRLMCAAEVIDKLHAICVVCGGPATRNQRLV
                     DGQPAPWNSPTIMVGGRESYEARCRHCHRVPRADEGQTALL"
     gene            3048..3665
                     /gene="coaE"
                     /locus_tag="AC16JC_01415"
     CDS             3048..3665
                     /gene="coaE"
                     /locus_tag="AC16JC_01415"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019065291.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR5163385.1"
                     /translation="MLNVALTGNIAAGKSTVIALFRRWGATIIDADELVREVEAPGTP
                     VLAAIARRFGDEVVAPDGSLDRAALRAKVLADGAALAALNALVHPAVERRRQALLDAA
                     RERGDRIVVSDIPLLFEVLDPQRFDAVVLVDAPVAVRHGRLRDLRGLSLEDADRLMAA
                     QLPAERKRARSQYVIDNDGTLENLERRARAVYAELERRAAAKQQA"
     gene            3733..4125
                     /gene="gcvH"
                     /locus_tag="AC16JC_01420"
     CDS             3733..4125
                     /gene="gcvH"
                     /locus_tag="AC16JC_01420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009581899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR5163386.1"
                     /translation="MANIPQDLRYTPEHEYVKSTGDPKVVQIGITDYAQGELGDVVFV
                     ELPKPGASFTKMQVFGTIEAVKAVSELYCPVAGTVVEANGALDGDPALVNRDPYGAGW
                     MIKLKVQDPAEVATLLDAAAYEKHIGSK"
     gene            4273..>6601
                     /gene="gcvP"
                     /locus_tag="AC16JC_01425"
     CDS             4273..>6601
                     /gene="gcvP"
                     /locus_tag="AC16JC_01425"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HIR5163387.1"
                     /translation="MSNSADLVHPDIFRTRHIGPRPEEQREMLALLGYDSIDAFIDAV
                     VPQDIRLRRPLALPPGRDERQVLQALRRMANQNQPFRSYLGMGYHACFTPQVIQRNVL
                     ESPGWYTSYTPYHAEISQGRLEALLTFQTMVADLTGLEIANASLLDEATAAAEAMHVT
                     EAVAGAKAHPVYCIDEQCHPQTIAVVRTRAEARGIEIVVGDPESFQFGDGVIGALVQY
                     PGTDGRLRDFRALCERAHKSGALVTAATDLLALTLLVPPGEWGADMAVGNSQRFGVPM
                     GYGGPHAAFFATRDAHKRHLPGRIIGVSKDRAGRPALRMALQTREQHIRREKATSNIC
                     TAQVLLAVMAGMYAVYHGPEGLRRIAERVHAFTALLAKALQRMGYRLAHDAYFDTLCI
                     VVEPWALPRLQDAAWARRINVRALPPDRIVVALDETVTVDDLSDLIAVFSMNETLPYM
                     ADEAAAKVETTIPPSLTRRSTYLTHPVFHRYRSETEMLRYLKRLEARDLSLTSAMIPL
                     GSCTLKLNATTEMVPVSWREFNRLHPFAPLSQAQGYQMLFRQLENQLAEITGFAAVSL
                     QPNAGSQGEFTGLLVIRAYHRSRGDTKRVTCLIPQSAHGTNPASAAMAGMQVVVVKTD
                     AQGNIDLDDLRAKAKQHAATLAALMVTYPSTHGVFEATIREVCRIVHANGGQVYMDGA
                     NLNAQVGLCRPADIGADVCHLNLHKTFCIPHGGGGPGMGPIAVARHLAPFLPDHPVVP
                     LGHAQSCGTVSAAPWGSASILPISWAYISLMGQEGQ"
BASE COUNT         1068 a         2368 c         2148 g         1017 t
ORIGIN      
        1 ctggccgagc tgcgcgtgct cgactgttcc cgcgtcctcg ccggcccgtg ggccggccag
       61 atcctggcag acctgggcgc cgacgtcatc aaggtcgagc ggcccggcac gggcgacgat
      121 acgcgccact ggggcccgcc ctatctcaga gatcagaccg gcaaggagac gagcgaggcg
      181 gcgtacttcc tgtgcgccaa ccgcggcaaa cgctccctga ccgtggactt cgccaccacg
      241 gacgggcgcg acatcgtgcg gcgcctcgcg gcccaatccg atgtcttcct cgagaaccac
      301 aaagtgggcg acctggcccg ccacgggctg gacttcgaga gcctgcgcgc gttgaatccg
      361 cggctcgtct actgctcgat caccggcttc ggccagagcg gaccccacgc gccccgggcg
      421 ggctacgact tcgcgatcca ggcgctgggc ggactgatga gcgtcacggg ggagcgcgac
      481 gaccggccgg gcggcggccc gcagaaggtc ggcgtggccg tcgcggacgt catggccggg
      541 ctgtacgcgg cgatcgccgt cctagcggcg ctgatgcacc gggagcgctc gggactgggg
      601 cagcacatcg atctcggact gctcgacgtg caggtggcca cgctcgccaa cgtggcgctg
      661 aactacctgg tgggaggaga ggtgccgcga cgctggggca atgcgcacgc caacatcgtc
      721 ccctaccagg tgctgcccac cgccgatcga cccatcgtgc tggtggtcgg caacgacgag
      781 caattccgga agttctgcga agtggcggga gcgccggcgc tggccgccga cgcccgatac
      841 gccacgaacg aggcgcgcgt gcgccatcgc gaggcgctga cgcgcgagct ggagcgcgtg
      901 ctcgcgaggc gctccgccgc tgcctggctc gccgcgctcg aaccggcggg cgtgccgtgc
      961 gcgccgatca acgacgtcgc ggaggtgttc gacgatccgc aggtgcgcca ccgcggcctg
     1021 cgcgtcgagg tgccgcatcc gctcgccgga accgtgccgc tcgtggcgaa ccccatccgc
     1081 ctgtcggagg cgcccattcg ctacgacgca ccgccgccgc tgctgggcca gcacacggac
     1141 gaaatcctgc gcgagcgcct cggcttcgcg ccggcggcga tcgccgacct gcggcgccgc
     1201 ggcgtggtgt gaggacggaa ctgcgagccc gcgctgaggt aatggaaacg accacatccc
     1261 cggaaggcat cgtgtccgac ggtgtaccca gacaacaggc attcctgctc aacggattca
     1321 cccagccgcc cggtcacggc agcgccaccg tgatcacgta cgaccggccc gcgcgctggc
     1381 agcgggcgct caaggccctc gggaaatggt ggggcatcgc gctcctgtcg gtgttcattc
     1441 ccgtagccca tttcatcctc gtcccttcgt tcttcctgtt cggcctctac cagttctcgc
     1501 agcgactcgg cgcggtggag gtccccgccg acgcgcacgg caagtgcccg gactgcggcg
     1561 ccgaacagcc gctcgagctc gcgtcccagt ggcatgtacc gcagctcgtc acctgtcacc
     1621 agtgtcaccg cgggctgcgg ctgacgttgc ccgacatgga caccaccgcc tgaaacgctg
     1681 acgggccgcc cagcggggcg gcccgtatgc agcgaacgat caactgacga ccgacgacca
     1741 aaaccgacta cttcttgttc accgccatct tcagctgttt gccggcgcgg aacgccgggg
     1801 ccaccgtggc cttgatctga atctcggcgc cggtcttggg attgcgcccc acacgcgcgg
     1861 cgcgcttgcg ggcctcgaag ttgccgaacc ccgtgatctg caccttcgcc cccttcttca
     1921 actcaccggc gatgatgccc ttgtcgtcga acagggcctc caccgcccgg ccggaatcgg
     1981 ccttggacat cttcgtccgc atcgccaacg cggcgatcag ctcggctttg ttcacagcgc
     2041 gctcctcact ggaaggtcga gagagggaaa aacccttgca aatccgcccc tttctcatgg
     2101 aaggcccgga aaagccttga aatctcggca caatatgggg gactaccccg gaaatccgca
     2161 agacatgggc cctttcccgg ccccccctcc cattgcggat tggagattgc ggattgcgga
     2221 ttgggccgtg gtggattctg ccttcagaag cccgtcgacc aatccccaat ccaatcccca
     2281 atccccaatc cgcaatccgc aatggaacgc cttgctctcc gactccgccc cggtgagctt
     2341 gcagccccat gttccacggt acgcgccccg gctggatcga agtcatcgcc ggcgtcatgt
     2401 tcgccggcaa gagcgaggag ctgattcgcc gcgtccgacg cgccgccatc gcgcgcaagc
     2461 aagtccaggt gttcaagtcc catctcgacg cccgctacgc cggcctgtac tccgtgacga
     2521 cgcacgacgg catcaccgtc gaggcggagc cggtggactg ctccgcgcag atcgtccgcg
     2581 cggtccggct cgacaccgag gtggtcgcgg tggacgaagc gcagttcctg gacgagggca
     2641 tcgtggacgt ctcgggcgcg ctggccgacc gcggcgtgcg cgtgatcctc gcgggcaccg
     2701 acgtggactt ccggggcctg cccttcgggc cgatgccccg cctgatgtgc gccgccgagg
     2761 tgatcgacaa gctgcacgcc atctgcgtcg tgtgcggcgg tcccgcgacc cgcaaccagc
     2821 gcctggtgga cggccagccc gcgccctgga acagtcccac catcatggtc ggcggccgcg
     2881 agagctacga ggcgcgctgc cggcattgtc acagagtccc gagggcggat gaggggcaga
     2941 cagcgctcct ctgattgcgg attggggatt gcggattgcg gattgatcgt tcgctttgaa
     3001 atccgcaatc cacaatccgc aacccgcaat tccttccctg gtacccgatg ctgaatgtcg
     3061 ctctgaccgg caacatcgcc gccggaaaat ccaccgtcat cgcgctgttc cggcgctggg
     3121 gcgccacgat catcgacgcc gacgagctgg tgcgtgaggt ggaggctccc ggcacgccgg
     3181 tgctcgccgc catcgcgcgt cgcttcggcg acgaagtcgt ggcacccgac ggcagcctcg
     3241 accgggccgc cctgcgcgcc aaggtgctcg ccgacggcgc agcgctcgcg gcgctcaatg
     3301 cgctggtgca tcccgcggta gagcgacgtc gccaggcgtt gctggacgcc gcccgcgagc
     3361 gcggcgaccg catcgtggtg agcgatatcc cgttgctctt cgaggtgctc gacccgcagc
     3421 gcttcgacgc cgtggtcctg gtagacgcac ccgtagccgt gcggcacggc cggctgcgcg
     3481 atctgcgcgg gctcagcctc gaagacgccg accgcttgat ggccgcgcag ctgcccgcgg
     3541 agcgcaagcg cgcgcgcagc cagtacgtga tcgacaacga tggcacgctg gagaatctgg
     3601 agcggcgcgc ccgcgcggtg tacgccgagc tcgagcggcg cgccgcggcg aaacagcaag
     3661 cttgacatgg gtttccacgg tccctagctt cgcccgcgtt cccgccaacc accagtcgag
     3721 agtgaccccg gcatggccaa tatcccccag gaccttcgct acactcccga gcacgagtac
     3781 gtcaaatcca ccggcgatcc caaggttgtg cagatcggga tcaccgatta cgcccagggt
     3841 gagctgggcg acgtggtgtt cgtggagctg ccgaagcccg gtgcctcgtt caccaagatg
     3901 caggtgttcg gcacaatcga agccgtgaag gccgtgtccg agctgtattg cccggtcgcc
     3961 ggaaccgtgg tggaagcgaa cggggccctg gacggcgacc cggccctcgt gaaccgggat
     4021 ccttacgggg cggggtggat gatcaagctc aaggtgcagg accccgccga ggtcgccacg
     4081 ctgctcgatg ccgcggccta cgaaaagcac atcggcagca aatgaagcgg acagctatca
     4141 gctatcagct atcagctatc agctatcagc tatcagctat cagctatcag ctatcagcaa
     4201 accatgacta cgtcgcttgc tgacggctga tggctttgag tccctgaact ccgaaccccc
     4261 ggtcaccctc gcatgtcgaa ctccgcagat ctggtccacc ccgacatctt ccgcactcgt
     4321 cacatcgggc cccggcccga agagcagcgc gagatgctgg cgctgctggg ctacgactcg
     4381 atcgacgcct tcatcgacgc cgtcgtgccc caggacatcc ggctgcgccg cccgctcgcc
     4441 ctgcccccgg gacgcgacga gcgtcaggtg ctccaggcgt tgcgccgcat ggccaaccag
     4501 aaccagccgt tccgctccta cctcggcatg ggataccacg cctgcttcac gccgcaggtg
     4561 attcagcgca acgtgctgga gagccccggc tggtacacgt cctacacgcc ctaccatgcg
     4621 gagatctcgc agggccggct cgaggcgctg ctgacgttcc agacgatggt ggcggatctg
     4681 accggcctcg agatcgcgaa cgcctcgttg ctcgatgaag cgaccgccgc ggcggaagcg
     4741 atgcacgtga ccgaggcggt ggccggcgcc aaggcgcacc cggtctactg catcgacgag
     4801 caatgccacc cgcagaccat cgcggtggtg cgcacccggg cggaagcccg tggcatcgaa
     4861 atcgtggtgg gcgacccgga gtcgttccag ttcggcgacg gagtgatcgg ggcgctggtg
     4921 caatacccgg gcaccgacgg ccggctgcgg gactttcgcg ccctgtgcga gcgcgcccac
     4981 aaatcgggcg ccctggtcac cgcggccacc gacctgctgg cgctcacgct gctcgtgccg
     5041 cccggcgaat ggggcgcgga catggcagtg ggcaacagcc agcgcttcgg cgtgccgatg
     5101 ggctacggcg gcccccacgc ggcgttcttc gcgacgcgcg acgcccacaa gcgccacctc
     5161 cccggtcgca tcatcggcgt ctcgaaggac cgcgccgggc gtccggcgct gcggatggcg
     5221 ctgcagacgc gcgagcagca catccgccgc gagaaggcca cgagcaacat ctgcacggcg
     5281 caggtgctgc tcgcggtgat ggcgggcatg tacgccgtct accatggtcc cgaggggctg
     5341 cgccgcatcg ccgagcgcgt gcacgcgttc accgccctcc tggccaaggc gctgcagcgg
     5401 atgggctacc ggctggcgca cgacgcgtac ttcgacacgc tgtgcatcgt ggtcgagccg
     5461 tgggccctgc cgcggctgca ggacgccgcc tgggcacggc gcatcaacgt ccgcgccctg
     5521 ccgcccgacc gcatcgtcgt ggcgctcgac gagaccgtca cggtggacga cctctcggac
     5581 ctgatcgccg tgttctcgat gaacgagacg ctgccctaca tggccgacga ggcagccgcc
     5641 aaggtcgaga ccacgattcc cccaagcctg acgcgccgct cgacctacct gacgcatccg
     5701 gtcttccacc ggtaccggtc cgagacggag atgctgcgct acctgaagcg gctcgaagcc
     5761 cgcgacctct cgctcacctc ggcgatgatc ccgctcggct cgtgcaccct caagctcaac
     5821 gccacgaccg agatggtgcc cgtgtcgtgg cgcgagttca accggctgca tcccttcgcg
     5881 ccgctgtccc aggcccaggg gtaccagatg ctgttccgcc agctcgagaa ccagctggcg
     5941 gagatcaccg ggtttgccgc ggtgtcgctg cagcccaacg ccggctcgca gggcgagttc
     6001 accggcctgc tcgtgatccg cgcctaccat cgctcccgcg gcgacaccaa gcgtgtcacc
     6061 tgcctcattc cgcaatcggc gcacggcacc aatcccgcca gcgccgccat ggcggggatg
     6121 caggtcgtgg tggtgaagac cgacgcgcag ggcaacatcg atctcgacga tctgcgcgcc
     6181 aaggccaagc agcacgccgc cacgctggcg gcactgatgg tgacctatcc gtccacgcac
     6241 ggcgtattcg aggccacgat ccgcgaggtc tgcaggatcg tccacgcgaa cggcggacag
     6301 gtgtacatgg acggcgccaa cctgaacgcc caggtcgggc tgtgccggcc ggcggacatc
     6361 ggcgccgacg tatgccatct caacctgcac aagacgttct gtatcccgca cggcggcggc
     6421 ggtcccggga tgggcccgat cgcggtcgcg cggcacctgg cgccgttcct ccccgaccat
     6481 ccggttgtcc cgctcggcca tgcgcaatcc tgcggcaccg tttccgccgc gccgtggggc
     6541 agcgcgtcca tcctgccgat ctcctgggcc tacatctctc tgatgggaca ggaaggacag
     6601 g
//
LOCUS       DBNGZW010000055         6814 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1436505, whole genome shotgun sequence.
ACCESSION   DBNGZW010000055 DBNGZW010000000
VERSION     DBNGZW010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6814)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6814)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6814
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1436505"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1938)
                     /locus_tag="AC16JC_01430"
     CDS             complement(64..1938)
                     /locus_tag="AC16JC_01430"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_function: GO:0031177 - phosphopantetheine binding
                     [Evidence IEA];
                     GO_process: GO:0019184 - nonribosomal peptide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0044550 - secondary metabolite biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HIR5163388.1"
                     /translation="MSERVPSILAGPARTLPDARAHEVFEDQARARPDALAAVHGARQ
                     LTYGELNARANRLAFALLERGLAREGVVGVVTERNLDWMTAVLAIFKSGGAYLPIEPH
                     FPADRIARTLSRAGCRLVLAERGSTGMLDQALASLPEVEQLLIDTALGEGHAEVNPRI
                     AVTPDQLAYIYFTSGSTGEPKGAMCEHAGFLNHLCAKIDDLRIDDQTVVAQTAPQCFD
                     ISLWQLLSALLVGGRTLIVEQETILDARRFIDRIVEGGVNVLQIVPSYLEVLVSHLER
                     HPHELTQLRFVSVTGEALKKPLVERWFAVQPRIRLVNAYGLTETSDDTNHEIMDRPPP
                     GDFVPLGRPINNVRISVVDEHLSPVPLGEPGEIVFSGVCVGRGYINDPERTAQAFLPD
                     PHRAGERLYRSGDYGRLLPEGALEFLGRRDAQVKISGFRIEIGEIENRLLRLPGVREG
                     AVVVTERADKGKQLVAFYSGERLDPAVVRDRLAASLPTYMVPATVHWRETLPLTGNGK
                     IDKKTLTALAGALEVAEERREAPSTATEHRLAAAWAAVLGIPEQQIGRRDHFFELGGT
                     SLSALRLAIALDRALSFKDLSVHPILADQAELVDARGAPSAPVLVPSGQCATTGGGVQ
                     "
     gene            complement(1940..4372)
                     /locus_tag="AC16JC_01435"
     CDS             complement(1940..4372)
                     /locus_tag="AC16JC_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014173714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904091 - non-ribosomal peptide synthetase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Pls/PosA family non-ribosomal peptide
                     synthetase"
                     /protein_id="HIR5163389.1"
                     /translation="MTTVVVPGVEAVLADLLAGVLRVEAVSVDSHFFDDLGADSLVMA
                     HFCARVRKRGDLPPVSMREVYRHPTIRSLAAALADVAPRTATPPASARIEAPTPTRGG
                     EYILCGTLQALFLLAYSYVAVVAIVAGYAWVAAGSSGGAIYQRLVLANVAAFMVVCAV
                     PVAAKWLLVGRWTPRQIRLWSLAYVRFWIVKTLVRSSPAARLFIGTPLYTLYLRALGA
                     KIGRGVVIFSRRVPICTDLLTIGAGTVIRGEALFNGYRAVAGRIETGPVTLGRDVFVG
                     ERSVLDIHTAMGDGAQLGHASALHSGQTVPKGERWHGSPAQRTDVSYVRVPPARVGTL
                     RRAGYAVAALLVVLLLYLPLMQTGFTLAMRAASSLADVVAPNAGAGVAALLIEAVLLS
                     LVLFWGLALIGLALMVAVPRVLNRFLTPDRVYPLYGFHHAVHRAIARLGEAKFFTFLF
                     GDSPYIVRYLQQLGYQLSPVVQTGSNFGIEVMHANPSLCSVGSGTMVADGLIMMNAEV
                     SSTSFRVSRVAIGRNNFLGNDITYPPGGRTGDNVLLGTKVMVPLDGEMREGVGLLGAP
                     PFEIPRTVERDTRFDHLRSGEALRRGLAAKARYNLRSIGVFLFTRWMSFVLFATLYLA
                     ALELYDAVPHVVSAVLFALSIVTTTVYFVLVHRCLDALHPTRPVICSIYHPDFWAVER
                     LWKVHPIHLLHAFDGTPFKNVLWRLMGARIGRRVFDDGTHIPEPSLTAIGDESVLNYR
                     SKIQNHSQEDGTFKCDRTTIGAGCTVGVDALIHYGVTMGDGAVLGTDSFLMKGEDVPA
                     RTRWSGNPAREL"
     gene            complement(4369..5382)
                     /gene="sbnB"
                     /locus_tag="AC16JC_01440"
     CDS             complement(4369..5382)
                     /gene="sbnB"
                     /locus_tag="AC16JC_01440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008952961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0019290 - siderophore biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnB"
                     /protein_id="HIR5163390.1"
                     /translation="MDFALSIINGKTVFDIIRDHRADCIDVVRDAYLAHDRGQSVNPD
                     SYFLRFPEKPDCRIIALPAYVGDGFHVAGLKWIASYPGNVQRGFPRASAVLVLNSYDT
                     GYPFAILESSIISAARTAASAALAAYWLNGQSRRARALGIVGTGFIARYVYDFLVETG
                     WEIEEVRLHDTSPHEGEKFRDTACRRERHRSVTVVPDAAELLRASDLIVFTTVASAPH
                     IADAALFAHNPLVLHISLRDLAPEILLASHNVVDDVEHVMKANTSPHLALQQTGNTSF
                     VTGTLAEIMLGRRSVDRSRPVIFSPFGMGILDVALGKWVYDRAVAADRAQRLTDFFYE
                     TAR"
     gene            complement(5387..6460)
                     /gene="sbnA"
                     /locus_tag="AC16JC_01445"
     CDS             complement(5387..6460)
                     /gene="sbnA"
                     /locus_tag="AC16JC_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HIR5163391.1"
                     /translation="MNRSATTATFPGLSSSDWERGTGGEVLATLPAVRREPVPGASLV
                     ARLEALGKTLRPTPHVRLAVEGMNLVAKLEYANPIGSIKDRPAFWILKRAAERGAIGD
                     GTTVIESSSGNFASALAAFTRMVGLSFIPVIDPNISSTYESFLRRLCPTVVKVEDRDD
                     TGGFLKTRLQMVRRLRGSIAKAYWTNQYGNPDAMDAHYRLTAGEICADFDSLDYVFIG
                     VSTGGTIAGVSRRLKERFPNVRIIAVDTQGSVIFGDAPRKRHIPGVGSSIVPPLLKHA
                     IIDDVVLIPERETVAACQELLTTHGLFAGGSSGTAFAAVKRYAPRMAGGWGKRPTVLF
                     LCADRGTPYLDTVFDPAWATTLE"
BASE COUNT         1098 a         2318 c         2386 g         1012 t
ORIGIN      
        1 gcggcggctt gccgggctgc tggtccaggt tgagcgttgc ggtcgcgggc cctctctcgc
       61 tggtcattgg actccccctc ccgttgttgc gcactggccg ctcgggacga gcaccggggc
      121 cgatggggcg ccccgggcgt cgaccagctc ggcctgatcg gcgaggatcg ggtgcacgct
      181 caggtccttg aaggacagcg cgcggtccag ggcgatcgcc agcctcaggg cggagagcga
      241 cgtcccgccc agctcgaaga agtggtcccg ccggccgatc tgttgctcgg ggatgcccag
      301 cacggcggcc cacgccgccg ccagccggtg ctcggtcgcc gtgctcggtg cctcgcggcg
      361 ctcctctgcg acctcgagcg ctccggcgag tgccgtcagc gtctttttgt cgatcttccc
      421 gttgcccgtg agcggcagcg tttcccgcca gtgcaccgtg gccgggacca tgtacgtggg
      481 cagcgacgcg gcgagccggt cccgcaccac ggccgggtcc agccgttcgc cggagtagaa
      541 ggccaccagc tgcttgccct tgtcggcccg ctcggtgacc acgaccgcgc cttcgcggac
      601 gccgggcagg cgcagcagtc ggttctcgat ctcgccgatc tcgatccgga agcccgagat
      661 cttgacctgc gcgtcgcgcc ggccgaggaa ctcgagcgcg ccctccggga gcaagcggcc
      721 atagtcgccg ctccggtata gccgctcgcc ggcgcggtgc ggatcgggca ggaacgcttg
      781 cgcggtgcgc tcgggatcgt tgatgtaccc gcggccgacg cacaccccgg agaagacgat
      841 ctcaccgggc tcgcccagcg gcaccgggga caggtgctcg tccaccacgg agatgcgcac
      901 gttgttgatg ggccggccca gcggaacgaa gtccccgggc ggcgggcggt ccatgatctc
      961 gtggttggtg tcgtccgagg tctcggtcag cccgtacgcg ttcaccagcc tgatgcgggg
     1021 ctggacggcg aaccagcgct cgaccagcgg cttcttcaag gcctcgccgg tgacggacac
     1081 gaaccgcagc tgcgtcagct cgtgcgggtg ccgctccaga tgggacacga ggacctcgag
     1141 gtacgacggc acgatctgca gcacgttgac gccgccctcg acgatccggt cgatgaagcg
     1201 ccgcgcgtcg aggatggtct cctgctccac gatgagcgtc cgcccgccga ccagcagcgc
     1261 ggacagcaat tgccacagcg agatgtcgaa acactggggc gccgtctggg ccaccactgt
     1321 ctggtcgtcg atccgcaagt cgtcgatctt ggcgcagagg tggttcagga acccggcgtg
     1381 ctcgcacatc gcacccttgg gttcgccggt cgacccggac gtgaaataga tgtacgcgag
     1441 ctgatcgggc gtgacggcga tcctcggatt tacctcggca tggccttctc ccaatgccgt
     1501 gtcgatcagg agctgctcca cttcggggag ggacgcgagc gcctggtcga gcatgccagt
     1561 gctgccgcgc tcggcgagca cgagccggca gcccgcccgg ctgagcgttc gggcgatgcg
     1621 gtcggcaggg aaatgcggct cgatgggcag gtatgcgccg ccggacttga agatcgcgag
     1681 caccgcggtc atccagtcga ggttccgctc ggtcacgacg cccacgacac cctcgcgggc
     1741 cagcccgcgc tcgagcaggg cgaacgccag ccgattggcg cgggcgttga gctcgccgta
     1801 ggtgagctgt ctcgcgccgt gcaccgccgc gagcgcatcg ggccgcgcgc gcgcctggtc
     1861 ctcgaagacc tcgtgggccc gggcgtcggg aagcgtccgg gccggtccgg cgaggatcga
     1921 cggcacgcgc tcgctcatgt cacagctccc gcgccgggtt gccgctccac cgcgtgcggg
     1981 ccggaacatc ttcacctttc atgagaaacg agtcggtgcc gagcacggcg ccgtctccca
     2041 tcgtcacgcc gtaatggatc agggcgtcga cgccgaccgt gcagccggcg ccgatcgtgg
     2101 tgcggtcgca cttgaacgtg ccgtcctcct gcgagtggtt ctggatcttg ctgcggtagt
     2161 tgaggacgga ctcgtccccg atggccgtga gcgacggctc ggggatatgg gtgccgtcgt
     2221 cgaacacccg ccgcccgatg cgggcgccca tcagccgcca cagcacgttc ttgaacggcg
     2281 tgccgtcgaa cgcgtgcagc aggtgaatcg gatgcacctt ccaaaggcgc tcgaccgccc
     2341 agaaatcggg gtggtagatg gagcagatga ccgggcgcgt cgggtgcaac gcgtcgaggc
     2401 agcggtgcac caggacgaag tagaccgtgg tcgtcacgat gctgagcgcg aacaatacgg
     2461 cgctgaccac gtggggcacc gcatcgtaca gctcgagggc ggcgaggtag agcgtcgcga
     2521 acaggacgaa gctcatccac cgggtgaaga ggaagacgcc gatgctccgc aggttgtacc
     2581 gtgccttcgc ggccaggccg cggcgcagcg cctcccccga gcgcagatgg tcgaatcgcg
     2641 tgtcccgctc gacggtgcgc gggatctcga agggcggggc gccgagcagc ccgactcctt
     2701 cgcgcatctc gccgtcgagc ggcaccatca ccttcgtccc gagcaggacg ttgtctcccg
     2761 ttcggccgcc cggggggtag gtgatgtcgt tccccaggaa gttgttgcgc ccgatcgcga
     2821 cccgggacac gcggaacgac gtgctcgaga cctcggcatt catcatgatg agcccgtcgg
     2881 ccaccatcgt gccgctcccg acggagcaca gggacggatt cgcgtgcatc acctcgatgc
     2941 cgaaattcga cccggtctgc accaccgggg agagctggta cccgagctgc tgcagatagc
     3001 ggacgatgta ggggctgtcg ccgaagagaa acgtgaagaa cttcgcctcg cccagccgcg
     3061 cgatcgcccg gtggaccgcg tggtggaagc cgtacagcgg atagacccgg tccggcgtga
     3121 ggaaccggtt caacacgcgg ggcacggcga ccatgagcgc gaggccgatc agcgcgagac
     3181 cccagaacag caccagcgag aggaggaccg cttcgatgag gagcgccgct acgcccgccc
     3241 ccgcgttcgg tgccaccacg tcggccaggg acgacgccgc gcgcatcgcc agggtgaagc
     3301 ccgtctgcat gagtggcagg tacaggagca ggacgacgag caaggcggcg acggcatagc
     3361 cggcccggcg cagcgtgccg acccgggccg gcggcactct cacgtagctc acgtccgtac
     3421 gctgcgccgg agatccgtgc caccgctcac ccttcggcac cgtctggccg ctgtgcaacg
     3481 ccgacgcgtg gcccagctgc gccccgtcgc ccatcgcggt gtggatgtcg agcacgctcc
     3541 gctcgcccac gaacacgtcg cggcccaggg tcaccggtcc cgtctcgatc cgccccgcca
     3601 cggcccggta cccgttgaag agcgcttcgc cgcggatcac cgttcccgcc ccgatcgtga
     3661 ggagatcggt gcagatcggc acgcgccgcg agaagatcac gacgccccgt ccgatcttcg
     3721 cgcccagggc ccgcagatag agcgtgtaca gcggcgtgcc gatgaacagg cgcgcggccg
     3781 ggctcgaccg aaccagggtc ttgacgatcc agaagcggac gtaggcgagg ctccacaggc
     3841 ggatctgccg cggagtccac cggccgacga gcagccactt ggcggcgacc ggaacggcgc
     3901 aaacgaccat gaacgcggcg acgttggcca gcaccagccg ctggtagatc gcgccgccgc
     3961 tcgagcccgc cgcgacccac gcatagcccg ctacgatggc gaccacggcg acataggagt
     4021 acgccagcag gaacagggcc tgcagcgtcc cgcagaggat gtactccccc ccgcgggtgg
     4081 gcgtcggcgc ctcgatcctc gccgaggccg gcggcgtggc cgtcctgggc gccacgtcgg
     4141 cgagcgccgc cgccaggctg cggatcgtgg gatggcggta gacctcccgc atggacaccg
     4201 gcggcaggtc tccacgcttg cgtacccgcg cgcagaaatg ggccatgacg agcgagtcgg
     4261 cgccgaggtc gtcgaagaaa tggctgtcca ccgacaccgc ttcgacgcgc aggacgccgg
     4321 ccagcaggtc ggcgaggacg gcctcgacgc cgggcaccac caccgtcgtc atcgcgcggt
     4381 ctcgtagaag aaatcggtca ggcgctgggc ccggtccgcg gccacggccc ggtcgtacac
     4441 ccacttgccg agcgccacgt cgaggatccc catgccgaac ggggagaaaa tgacgggacg
     4501 gctgcgatcg accgatcggc gtcccagcat gatctcggcc agggtgccgg tcacgaagct
     4561 cgtgtttccg gtctgttgca gggcgaggtg gggcgaggtg ttcgccttca tgacgtgctc
     4621 gacgtcgtcg accacgttgt gggatgcgag caggatctcc ggcgcgaggt cgcgcagcga
     4681 aatgtgcagc accagcgggt tgtgcgcgaa gagggccgca tcggcgatgt gcggcgccga
     4741 ggcgaccgtc gtgaagacga tcaggtccga ggcccgcagc agctcggcgg cgtcggggac
     4801 gaccgtgacc gagcggtggc gctcgcggcg gcacgccgtg tctcgaaact tctcaccctc
     4861 gtgcggcgac gtgtcgtgga gccgtacctc ctcgatctcc caccccgtct cgaccaggaa
     4921 gtcgtacacg tagcgcgcga tgaacccggt gcccacgatg cccagcgcac gcgcccggcg
     4981 cgattgtccg ttgagccagt aggcggccag cgcggccgac gcggcggtcc gcgccgccga
     5041 gatgatcgag ctctcgagaa tggcgaaggg gtagccggtg tcgtagctgt tgagcacgag
     5101 cacggccgac gcgcgcggga agccgcgctg cacgttgccc ggataactgg cgatccactt
     5161 gagccccgcc acgtggaacc cgtcgcctac gtacgccggc agcgcgatga tccggcagtc
     5221 gggcttctcg ggaaaccgca ggaagtagct gtccgggttg acggactggc cgcggtcgtg
     5281 cgcgagatac gcgtcccgca cgacgtcgat gcagtccgcc cgatggtcgc ggatgatgtc
     5341 gaagacggtc ttgccgttga tgatcgacag cgcgaagtcc atggccttac tcgagcgtcg
     5401 tggcccaggc gggatcgaac acggtatcga ggtacggcgt cccgcggtcc gcgcacagga
     5461 acagcacggt gggccgcttg ccccaccccc cggccatccg cggcgcatag cgcttgaccg
     5521 ccgcgaacgc cgtcccgctc gagccgccgg cgaacagtcc gtgggtcgtg agcagctcct
     5581 ggcacgccgc cacggtctcc cgctcgggga tcaggacgac gtcgtcgatg atcgcgtgct
     5641 tcaggagcgg cgggacgatg ctggacccca cgccggggat atggcgtttg cgcggggcgt
     5701 ccccgaagat gacggagccc tgggtgtcca ccgcgatgat ccggacgttc gggaatcgtt
     5761 ccttcagccg gcgtgacacg ccggcgatcg ttccccccgt gctgacgccg atgaacacgt
     5821 agtccagcga gtcgaaatcg gcgcagatct cgccggccgt gagccggtag tgcgcgtcca
     5881 tggcgtccgg attgccgtac tggttcgtcc agtaggcctt cgcgatggag ccgcgcaggc
     5941 gccgcaccat ctgcagccgc gtcttgagga atccgccggt gtcgtcgcgg tcctcgacct
     6001 tcaccacggt ggggcagagg cgccgcagga acgactcgta ggtgctggag atgttggggt
     6061 cgatcaccgg gatgaagctg aggccgacca tgcgcgtgaa ggcggcgagg gcggaggcga
     6121 agttgccgga ggaggactcg atcacggtgg tcccgtcccc gatcgccccg cgctcggcgg
     6181 cccgcttcag gatccagaac gcggggcggt ccttgatgct gccgatcggg ttcgcatact
     6241 cgagcttggc gacgaggttc atgccctcca cggccagccg cacgtgcggg gtgggccgca
     6301 gcgtcttccc gagcgcttcc aggcgcgcga cgagactcgc ccccggcacg ggctcccggc
     6361 ggacggccgg aagcgtggcg agtacctcac cccctgtccc ccgctcccaa tcggaggagg
     6421 agagacccgg gaacgtcgcg gtagttgccg atcgattcat ttagaattcc actcccgtcg
     6481 aaaatccgat gaggacgaag gaaagcccat ctcaacgcgt ttgacacacg acgcccggtt
     6541 ttgatcccgg ccccaccgca gcacgcactc cgatagtcac gacatcgacg cgctgttcgg
     6601 tgaacggacg gaacgggccg acgtcgtacg agaggcacag ccggccttca accgggtggt
     6661 cgcgatccgc tcaggagatc gcagtagcag cacagcgtga tcgcgggggc gacgctgagc
     6721 tccaccccgc gctcatgcga gtcatcaact cgcgtgccgc gacagcgtcg cgtcgtgtat
     6781 cggtttacgt ggttgtggca gtgcacgtta tgag
//
LOCUS       DBNGZW010000056         3521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_550040, whole genome shotgun sequence.
ACCESSION   DBNGZW010000056 DBNGZW010000000
VERSION     DBNGZW010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_550040"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1346
                     /locus_tag="AC16JC_01450"
     CDS             <1..1346
                     /locus_tag="AC16JC_01450"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR5163392.1"
                     /translation="AVVYIVSEIRQRADTRRLPPGFDDFFPQFRNRPQIEQGSGSGFI
                     VSPDGFILTNNHVVAGASKVTVRLYDKREFDAHVVGTDPQTDIAVIKIDAAALPTVAF
                     GNSDSTRIGEWVLAIGNPLGEAFAFTVTAGIVSAKGRLLSALQESRYSIQDFIQTDAA
                     INPGNSGGPLVNVHGEVIGINSAIASETGYYAGYGFAIPINLVRTVMTQLVASGHVQR
                     TVMGIGIKQATPEDAEAVGLKQITGVVVESFTNDESPAKRAGLELGDVITELDGQPVE
                     YVAQLQQRVGFKKPGELVRVTVLRQGGVRKEISVRRVAQPADNATQVSSNDGAGNNGD
                     ELSGSTNRLGIAIEPLTQEDAAQDPRLLRVARSGGALVVTDVSPDGPAYRKLFGNGPS
                     NSPDLILQVNGTPVHTRTEFRTAIQSVRPGGIATLQVLRRFPDGWDTAVVRVRAP"
     gene            complement(1407..2048)
                     /locus_tag="AC16JC_01455"
     CDS             complement(1407..2048)
                     /locus_tag="AC16JC_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163393.1"
                     /translation="MRRSAFYALCLLAGALLFGVAGLLHPVLRGDGAAQLATIARTPQ
                     WRLIHWSLLFGLPLMLAGLAGFVEQHRESPGAAPARAGIVVATFAFGVWGLNILFMAG
                     AGSHLAATYMASEPGLASTHAVFLYDMLHPFGLAAERLATFALGLAFYLLGWAGWNGR
                     LVPRWLAWGAFGAGAACMVIALAVAETSVVLFYGQAAAVIWMALAGVLLLVRR"
     gene            2139..2636
                     /locus_tag="AC16JC_01460"
     CDS             2139..2636
                     /locus_tag="AC16JC_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163394.1"
                     /translation="MKLTRVLREGFWAGCIGAGAVAAWFFVVDLVNGQAFFTPAMLGS
                     AVFWGATGPAQVVIEYSRVVGYTMIHVSAFVVIGTIAAALMAEAEDSPPALYLAVVLA
                     CCFEVGFYVLVAILAQPLLGALAWWSVAIGNGIAASAMGYYLWRQHPAIALELKKHPL
                     GATQD"
     gene            complement(2602..3456)
                     /locus_tag="AC16JC_01465"
     CDS             complement(2602..3456)
                     /locus_tag="AC16JC_01465"
                     /inference="COORDINATES: protein motif:HMM:NF021214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR5163395.1"
                     /translation="MAPAAPLPEAWGHWDLHPSVVVGLVLLGGLYVFLGGLQAPRRRV
                     AAFAASLLVLLASLNGPLHDLSDEYLFSAHMVQHLVLTLLFPPLFLYGLPAALVRKVI
                     RPAWLQAAGAVLVRPVPAAAIFSAPIILWHLPPFYEAAMRHHGLHIVQHLVFLATAVV
                     MWWPVLSPVPELPRASYPGQMLYLFLLGIPMSIVGAMITLADAPLYPFYAAAPRVWGL
                     SPLQDQQIGGLIMWVPGGLVFWAAMTVVWFRWARREERGEVERRVPLEAYGLPAGASP
                     ALPPGDAS"
BASE COUNT          609 a         1223 c         1229 g          460 t
ORIGIN      
        1 cggcggtggt gtacatcgtc tccgagatcc ggcagcgtgc ggacacccgc cggctgccgc
       61 ccggattcga cgatttcttc ccgcagttcc ggaaccgtcc ccagatcgaa cagggctctg
      121 ggtccgggtt tatcgtgtcg cccgacggct tcattctcac caacaaccac gtcgtcgccg
      181 gcgcgagcaa ggtcacggtc cggctgtacg acaagcggga gttcgatgcg cacgtcgtgg
      241 ggactgatcc gcaaaccgat atcgccgtca tcaagatcga cgccgccgcg ctgccgaccg
      301 tggcgttcgg caactccgac tcgacccgca tcggcgagtg ggtgctggcg atcggcaatc
      361 cgctcggtga agccttcgcc ttcacggtga cggccgggat cgtgagcgcc aaaggccggc
      421 tgctgtcggc gctccaggag tcgcgctact cgatccagga tttcatccag accgacgcgg
      481 cgatcaaccc cggcaattcc ggcggtccgc tcgtcaacgt gcacggtgag gtgatcggca
      541 tcaattcggc catcgccagc gaaacgggct actacgcggg ctacgggttc gccatcccga
      601 tcaacctagt tcggacggtg atgacccagc tcgtggccag cggccacgtg cagcgcaccg
      661 tcatgggcat cgggatcaag caggccacgc cggaagacgc cgaggccgtc ggcttgaagc
      721 agatcaccgg cgtcgtggtg gagagcttca ccaacgacga gtcccccgcc aagcgcgccg
      781 ggctcgagct cggcgacgtg atcaccgagc tggacggcca gccggtcgaa tacgtcgcgc
      841 agctgcagca gcgcgtcggg ttcaagaagc caggcgaatt ggtccgggtg accgtgctgc
      901 ggcagggagg cgtccgcaag gagatctcgg tgcggcgcgt ggcccagccc gccgacaatg
      961 cgacgcaggt ctcctcgaac gacggcgccg ggaacaacgg cgacgagctc tcgggctcga
     1021 ccaaccggct ggggatcgcg atcgagcccc tgacccagga ggacgccgct caggacccga
     1081 ggctgctgcg ggtcgcccgc tcgggtgggg cgctcgtggt gaccgacgtt tcgccggacg
     1141 gtccggccta tcgcaagctg ttcggcaacg gcccgagcaa cagcccggac ctgatcctgc
     1201 aggtcaacgg cacgccggtc cacacgcgca ccgagttccg gacggccatc cagagcgtgc
     1261 gcccgggcgg catcgccact ctccaggtgc tgcgccgctt cccggacggc tgggacacgg
     1321 cggtcgtgcg cgtgcgggct ccctgagcct caggagcggc tgcgaaacgg cgggcgccgg
     1381 caccacatgc ggcgcccgcc gtttgcttat cgccggacca gcaacagcac gcccgccagc
     1441 gccatccaga tgaccgccgc cgcctggccg tagaacagga cgacggaggt ctccgccacc
     1501 gccagcgcga tgaccatgca ggcagcgcct gcgccgaacg caccccaggc cagccagcgg
     1561 gggaccaggc gtccgttcca gcccgcccac cccaggaggt agaaggccag ccccagcgcg
     1621 aaggtcgcga gccgttcggc ggcgaggccg aacgggtgca gcatgtcgta caggaagacc
     1681 gcgtgcgttg acgccaggcc cggctcggag gccatgtagg tggcggcgag gtgcgacccc
     1741 gcgcccgcca taaagaggat gttgagcccc cacacgccaa acgcgaacgt ggccacgaca
     1801 atccccgcac gggcgggcgc cgcgccgggc gattcgcggt gctgctcgac gaacccggcc
     1861 agtccggcga gcatcaacgg caggccgaac agcagggacc agtgtatcag acgccactgg
     1921 ggcgtgcgcg cgatggtggc cagctgcgcc gcgccatccc ctcgcagcac cggatgaagc
     1981 agtccggcga cgccgaacag cagcgcgccc gccaggaggc agagggcgta gaacgcggac
     2041 cgtcgcatgt cgaacctccc tggagtcgcc gcgcgctaat ctagcccgtg gtgggggtgc
     2101 ccgtatattg gctcgacgca ccgtcgttgg agggccgcat gaaactgacg cgagtgttgc
     2161 gcgaagggtt ctgggcggga tgcatcgggg cgggcgccgt ggctgcctgg ttcttcgtcg
     2221 tcgacctggt gaacggccag gcgttcttca cgcccgccat gctgggctcg gccgtcttct
     2281 ggggcgcgac cggcccggcc caggtcgtga tcgagtactc acgggtcgtg ggctacacca
     2341 tgattcacgt cagcgcgttc gtggtgatcg ggacgatcgc cgcggcgctg atggcggagg
     2401 ccgaggattc accgccggcg ctctacctgg cggtcgtcct cgcctgctgc ttcgaggtgg
     2461 ggttctacgt gctcgtcgcg atcctggctc agccgctgct gggcgcgctc gcctggtgga
     2521 gcgtggcgat cggcaacggg atcgcggcca gcgccatggg ctattacctc tggcggcagc
     2581 accccgccat cgcgctcgag ctcaagaagc atcccctggg ggcaacgcag gactagcgcc
     2641 ggcggggagc ccgtaggctt cgagcggtac ccgccgctcg acctcccccc gctcctcacg
     2701 ccgcgcccag cggaaccaga cgacggtcat cgccgcccag aacaccagcc cgcccggcac
     2761 ccacatgatc agcccgccga tttgctgatc ctgcagcggg gagaggcccc acacccgcgg
     2821 cgcggcggca tagaacggat acagcggcgc gtcggcgagg gtgatcatcg ctcccacgat
     2881 cgacatcgga atgccgagca ggaacaggta gagcatctgc cccggatacg aggcgcgcgg
     2941 cagctccggg accggtgaca gcaccggcca ccacatgacg acggcggtgg cgaggaacac
     3001 cagatgctgc acgatgtgca gcccgtggtg acgcatcgcg gcctcgtaga acggcggcag
     3061 gtgccagagg atgatcggcg ccgagaagat ggcggccgcc gggacggggc ggaccaggac
     3121 ggcgcccgcg gcctgcagcc aggcgggccg gatcaccttg cggaccagcg cggcgggcag
     3181 gccgtagaga aacagcggcg ggaacagcag cgtcagcacc aggtgctgca ccatgtgggc
     3241 gctgaagagg tactcgtccg acagatcgtg cagcgggccg ttgagcgacg ccagcaggac
     3301 caggagcgag gccgcgaacg ccgcgacccg gcgccgcggc gcctgtagcc cccccagaaa
     3361 cacgtagagc ccgcccagca ggacgagccc cacgacgacg ctcgggtgca gatcccaatg
     3421 cccccacgct tccgggaggg gggcggcggg cgccacgcgc actcaggact tcagggcgaa
     3481 caccccgaag aggaacagca gtcccagcat cacgaccgcg g
//
LOCUS       DBNGZW010000057         3800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_686287, whole genome shotgun sequence.
ACCESSION   DBNGZW010000057 DBNGZW010000000
VERSION     DBNGZW010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_686287"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1578)
                     /locus_tag="AC16JC_01470"
     CDS             complement(4..1578)
                     /locus_tag="AC16JC_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HIR5163396.1"
                     /translation="MTALSLPAAVPADRYRYRYIIAITVSLASVLELLDTSIVNVAIP
                     HMMGSLGATLDEIAWVSTGYIVANVIVLPITGWLSAYFGRQRYFATSIAVFTIASFLC
                     GNSHTLAELVLWRIVQGLGGGALLSTSQAILYEEFPREEYGTAMAIFGMGVMVGPTLG
                     PTLGGYITDAAGWPWIFYINIPFGILALALTLSFIKDSRHQERAERVDYVGLLLLAAG
                     IGALQTMLERGERLDWFDSKGVTALAIVSVFSLATFVWHELRTEHPVVDLRILKSRQL
                     AVGVLFAAVLGVCLYATVFVLPVYLQNLQGFTAEQTGFVILPGALASAFTMGVMGRLS
                     RKFDPRLSIVAGVGLFALSMWKHAHFTTDSGMSDFFWPLIFRGVGLGLIFVPLTNLAL
                     ADLPLSRIPNGTGLFNLTRQLGGSVGIALSATLVTRFANVHRADLIADVTLYSPATQE
                     RLAMLQHGLAAQGVPATLLHTKAVAILNAIVQKQALMLSFEQLFLLFGAAFLVSLPLL
                     LLMHGSRGGPGGAVAH"
     gene            complement(1586..2626)
                     /locus_tag="AC16JC_01475"
     CDS             complement(1586..2626)
                     /locus_tag="AC16JC_01475"
                     /inference="COORDINATES: protein motif:HMM:NF052759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR5163397.1"
                     /translation="MEPDVEQQRPSRAGPAKSTPAEERPAAPGGRRRIVFAMLGMALL
                     ALIAGGVRKLIWARSHEATDNAQVDGHILPVLPKVGGFVTTVRVEENQPVRAGDTLVV
                     IDPRDYQVRLQQADADLAVALATVSSRQRVGQADAAVRQAEAAAEKAHADLDRLTPLA
                     AQGIVSRQQLDAAQTAVRSADAALAAAQAALLGADARVASARAARDQAALQLSYTHIV
                     APNDGLVSKKTVEVGELVQPGQPLMSVVPLQAVWVTANLKETQIEDVTPGDRVDFTVD
                     AYPGVHFRGIVESLSPATGARFSLLPPDNATGNFTKVVQRIPVRIRPDGPVDTAHPLR
                     PGMSVDVTITTK"
     gene            complement(2673..>3800)
                     /locus_tag="AC16JC_01480"
     CDS             complement(2673..>3800)
                     /locus_tag="AC16JC_01480"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR5163398.1"
                     /translation="LPDLSATGSWVSQTVNPQAFGFRFPGLALPRILGPFDLYDGRLH
                     LSQALFDHAGSVRVSASRSLLASAQSDSGLATEAAAQLTALDYLRLVRAQAVVAARQA
                     DSALAAELVRLAQAQKDAEVGTAIDVTRAQTQLVASQGALVVARNERERAHIDLVRSL
                     GLPPGTPIEPTDSLAATLPVAELPAGRDSLVALALARRPDLQSEQARGEAARRTASAI
                     SAERLPRLDLAANYGAIGLAPLDAIATRQVAVQVTVPILDGFRRESRHAEQEAVVRGS
                     EVRADELRRQIAAEVDAAQLDLASAGEQQTIARERLALAQEELDQARERFKAGVAGNI
                     EVIQAQSSLVRARDTDIDARFAAAVARVALARAVGVARLLH"
BASE COUNT          591 a         1372 c         1305 g          532 t
ORIGIN      
        1 ccatcagtgc gccaccgcgc cgccggggcc cccgcgactc ccgtgcatca ggagcagcag
       61 cggcagcgag accaggaagg cggcaccgaa cagcaggaac agctgctcga acgagagcat
      121 cagggcctgc ttctgtacga tggcgttgag aatggccacc gccttggtgt gcagcagcgt
      181 cgccgggacg ccctgcgccg ccaggccgtg ttgcagcatg gcgagccgct cctgagtcgc
      241 cggagagtag agcgtcacat cggcaatgag gtccgcgcga tggacgttcg cgaaccgggt
      301 cacgagggtc gccgagagcg cgatccccac gctgcctccc agctggcgtg tcaggttgaa
      361 gagcccggtc ccgttcggga tccggctcag cggcagatcg gcgagggcga ggttggtcag
      421 cgggacgaaa atcaggccca ggcccacccc gcggaagatc aggggccaga agaagtcgga
      481 catgccgctg tcggtcgtga agtgcgcgtg cttccacatc gacagagcga acaacccgac
      541 gccggcgacg atcgacagcc tgggatcgaa tttccgcgac aggcggccca tcacccccat
      601 ggtgaaggcg ctcgccagtg cgcccggcag gatgacgaac ccggtctgct cggcggtgaa
      661 cccctggagg ttctgcaggt acacggggag cacgaatacc gtggcgtaca agcacacgcc
      721 gagcacggcg gcgaacagca ctcccacggc cagctgccgg ctcttgagaa tccgcaggtc
      781 caccacggga tgctcggtgc gcagctcgtg ccacacgaac gtcgcgaggg agaacacgct
      841 cacgatcgcc agcgccgtca cgcccttcga atcgaaccag tcgagtcgct ccccccgctc
      901 cagcatcgtc tgcagcgcgc cgatcccggc cgcgagcagc agcagaccca cgtagtccac
      961 ccgctcggcc cgctcctggt ggcgcgagtc cttgatgaag ctcagcgtca gcgccagcgc
     1021 gaggattccg aacgggatgt tgatgtagaa gatccagggc cagccggcgg cgtcggtgat
     1081 gtagccgccc agtgtcgggc cgagggtcgg gccgaccatg acgcccatcc cgaagatcgc
     1141 catcgcggtg ccgtactctt cgcgcgggaa ctcctcgtac aggatcgcct gcgatgtgga
     1201 cagcagcgct ccgcccccca gaccctggac gatgcgccac aggaccagct cggccagtgt
     1261 gtgggagttg ccgcacagga acgacgcgat cgtgaacacg gcgatggacg tggcgaagta
     1321 gcgctgccgg ccgaagtagg ccgacagcca gcccgtgatc ggcaggacga tgacgttcgc
     1381 cacgatgtag ccggtcgaga cccacgcgat ctcgtccagc gtcgccccga gcgagcccat
     1441 catgtggggg atcgccacat tgacgatgct ggtgtcgagc agctcgagca ccgacgccag
     1501 cgaaacggtg atcgcgatga tgtagcggta gcgataccgg tccgccggca cggcggcggg
     1561 cagcgacaac gcggtcatgc ccaggtcact tcgtcgtgat ggtgacgtcc acgctcatcc
     1621 ccggccggag cggatgcgcg gtgtccacgg gtccgtcggg acggatccgc accgggatgc
     1681 gctgcaccac cttcgtgaag ttcccggtgg cgttgtccgg cgggagcagg gagaagcgcg
     1741 cccccgtcgc gggcgacagg ctctcgacga tcccgcggaa gtggacgccc ggataggcgt
     1801 cgacggtgaa gtccacgcgg tcgcccgggg tcacgtcctc gatctgcgtc tccttgaggt
     1861 tcgccgtgac ccacacggcc tggagcggca ccacgctcat gagcggttgc cccggctgca
     1921 ccagctcgcc cacttccacc gttttcttgc tgaccagacc gtcgttcggc gcgacgatgt
     1981 gcgtatagga cagctgcagg gcggcctggt cgcgcgcggc ccgggcggaa gccacacggg
     2041 cgtcggcgcc caacagcgcc gcctgggccg ccgccagcgc ggcgtccgcc gagcgcaccg
     2101 ccgtctgggc ggcgtcgagc tgctgccggc tcacgatgcc ctgcgccgcg agcggcgtca
     2161 gccggtcgag atccgcgtgc gccttttcgg cggcggcttc ggcctgccgg acggcggcgt
     2221 cggcctgccc cacgcgctgg cgcgagctga cggtcgccag ggcgacggcc aggtcggcgt
     2281 ccgcctgctg cagccggacc tgatagtccc gggggtcgat cacgaccagg gtgtcgcccg
     2341 cccggaccgg ctggttttcc tcgactcgca ccgtggtcac gaagccgccg accttcggca
     2401 gcaccggaag gatgtggcca tccacctggg cgttgtcggt cgcctcgtgg gaccgcgccc
     2461 agatcagctt gcgcacgccg cccgcgatga gtgccagcag cgccataccc agcatggcga
     2521 acacgatccg gcgccgtcca ccgggagcgg ccggccgttc ctcggccggc gtgctcttcg
     2581 cggggccggc ccgggagggc cgctgctgct ctacgtcagg ttccatcgtc gtagccatga
     2641 tcgtcctctg gtctcggtag ctctgtttgc cgtcaatgga gcagccgggc gacgcccacg
     2701 gcgcgtgcca gcgccacgcg ggccaccgcg gccgcaaacc gtgcatcgat gtccgtatcc
     2761 cgggcccgca ccagggaaga ctgcgcctgg atcacttcga tgttccccgc gacccctgcc
     2821 ttgaagcgct cacgggcctg gtcgagttcc tcctgggcca gcgccaggcg ctcgcgcgcg
     2881 atcgtctgct gctcgccggc cgacgccagg tcgagctgcg ccgcatccac ctccgcggcg
     2941 atctgccgcc gcagctcgtc ggcccggacc tccgatcccc gcaccaccgc ttcctgctcc
     3001 gcgtggcggc tctcacggcg gaacccgtcc agaatcggca ccgtcacctg caccgccacc
     3061 tgccgcgtcg cgatcgcgtc gaggggcgcc agcccgatgg cgccgtagtt cgccgcaagg
     3121 tcgaggcgcg gcagccgctc ggccgaaatc gccgacgcgg tgcgacgcgc ggcctctccc
     3181 cgcgcctgtt ccgattggag gtcgggacgg cgtgccagcg ccagcgccac gagcgagtcg
     3241 cgcccggcgg gcagctccgc aaccgggagc gtcgccgcca gcgagtcggt cggctcgatc
     3301 ggcgtgccgg gaggcaggcc gagactccgg accaggtcga tgtgggcgcg ctcgcgctcg
     3361 ttccgtgcga ccaccagcgc gccctgcgag gcgacgagct gcgtctgcgc gcgggtcacg
     3421 tcgatcgccg tccccacctc ggcgtccttc tgcgcctgcg ccaatcgcac cagctccgcc
     3481 gccagcgcgg aatcggcctg ccgcgcggcg accaccgcct gcgcccgcac gagcctgagg
     3541 tagtcgagcg cggtcaactg agcggccgcc tccgtcgcca gcccgctgtc ggactgggcg
     3601 ctcgcgagca acgagcggct cgcactgacc cgcacgctgc cggcgtggtc gaacagcgcc
     3661 tgcgacagat gcagccgccc gtcgtacagg tcgaacggcc cgaggattct cggcagcgcg
     3721 agccccggaa acctgaaacc gaacgcctga ggattgactg tctggctgac ccagctcccg
     3781 gtggccgaga gatcgggcag
//
LOCUS       DBNGZW010000058         4881 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1300239, whole genome shotgun sequence.
ACCESSION   DBNGZW010000058 DBNGZW010000000
VERSION     DBNGZW010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4881
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1300239"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2062
                     /locus_tag="AC16JC_01485"
     CDS             <1..2062
                     /locus_tag="AC16JC_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020570433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR5163399.1"
                     /translation="IGESKMAWEPAWSPDGKQIAFEDNKVRIRVVDVESGRIRTVDAG
                     GVNIERGGMELAWSPDSRYLAYAKTYPNMFRRIVVWSRDSGSVTPLTDALADAAAPVW
                     DRNGRHLYFLASTNLALGSGWANTSSIAADPSYGVYLMVLRADDPTPFAPRSDEEGDT
                     TAAKQGSDTGAVQVRIDRAGIERRIVALPMPVRRYTDLFAGPKGTVFVAERVENQTGM
                     VLQKFSQADRKATEFVRGATRISVSPDGEKILFRSGTQWRVVGTAHTPDAGAGNVAVS
                     LRMYLDRAGEWRQIFDETWHYERDFFYDPGMHGNDWNAVRERYRPLVPWVRHRDDLSY
                     ILDLVNGELSVGHSFVFGGDMPVVDTSGVGVLGADLVTDAGRWKIARIYTFESWNPDL
                     VAPLDRPGLRIRVGQYIFGVDGRELTAADDPYRLLDGTAGRQTVLLVNDRPTMDGHWT
                     VTVEPAQNENQLRQRAWVEDNRRTVDSLSGGRLAYVWIPNTGGPGVVSFNRYFFAQQD
                     REGAVIDERFNGGGNLDDYMVDYMTRSLRAAITNEVPNGAPFRLPAGILGPKVLLINE
                     LAGSGGDYFPWAFRQQQAGPLVGARTWGGLVKSSVHYPLVDGGAVTAPDNAVFDPINN
                     KWIAENEGVPPDVEVLLDARSVAAGRDPQLERGVQEALRLLQARGTQRVTPPPFSRPS
                     RRPR"
     gene            2065..4533
                     /locus_tag="AC16JC_01490"
     CDS             2065..4533
                     /locus_tag="AC16JC_01490"
                     /inference="COORDINATES: protein motif:HMM:NF014850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HIR5163400.1"
                     /translation="MRILRIALLVAFALPELAAAQRQRLSMDPGWRFTLGDPAGAQQP
                     AFDNHQWRRLDLPHDWSVEGTPQQDAPSGGRGGYFPTGLGWYRKAFRLPATARGREVW
                     LEFDGVYMNSDVWINGAHLGKRPYGYSTFAYDITKHVVQGVNVVAVRVDNSAQPNSRW
                     YTGSGIYRHVWLTIVDPLHVGHWGVYVTTPRVDSSSADVVVRTHVENDNPAVRRTVLR
                     STVIDGSGHEVAHADTSISVGIGGSTDVEQHIVVPSPQLWSVERPTMYSLRTELLTAS
                     TARPPIDVLASPFGIRTIAYDTDKGFLLNGRQVKMRGVNLHHDAGALGAAVPERIWEM
                     RLERLKAMGANAIRTSHNPPAPEFLDLCDRLGLLVMAEAFDEWTIGKVPEGYHKYFAE
                     WSERDVTDFVHRDRNHPSIVLWSAGNEIGEQTTPDGVDVLRRLVGIFHREDPTRPVTT
                     GNDQIYADGHPATPAFLEAEDIVGYNYVDRWHERRELFAEQDRHDHPDWKMIGTESGS
                     LFQSLDERYSLGDDSTVVRPNYVSGMTQAERLWKWVTLHDYFAGNFMWTGIDYLGESF
                     WPFKGFGSGTMDITGQPKDAYYLYQSLWTDQPVLHLFPHWNWTGREGQVIPVVAYSNC
                     NSVELFLNGRSLGEKRIEFPAQGTSGGWNSYALPVVRSTTNDLHMSWDVPYEPGVLRA
                     VGKRRDGTVGCEAEVRTAGAPAAIRLLPDRDTITTGAGDVALVAFEIVDSAGTVVPTA
                     GNLVHVTVTGGTLVALDNADMRDHDPYRTDRRHAFNGRGLALLLGLQTGVMRVTASAD
                     GLRDGSVMVVVRAGKAAAVIQAAR"
     gene            complement(4732..4815)
                     /locus_tag="AC16JC_01495"
     tRNA            complement(4732..4815)
                     /locus_tag="AC16JC_01495"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4779..4781),aa:Ala,seq:cgc)
BASE COUNT          801 a         1650 c         1701 g          729 t
ORIGIN      
        1 gatcggcgag tcgaagatgg cgtgggagcc cgcctggtcg cccgacggga agcagatcgc
       61 cttcgaggac aacaaggtca ggatccgcgt ggtggacgtc gagtcggggc ggatccgcac
      121 tgtggatgcg ggcggcgtca acatcgagcg cggcgggatg gagctcgcct ggtcgcccga
      181 ctcccgctac ctcgcctacg ccaagacgta tcccaacatg ttccgccgca tcgtggtctg
      241 gtcgcgggac agcggcagcg tcaccccgct caccgacgcg ctggcggacg ccgccgctcc
      301 cgtatgggac cgcaacgggc ggcatctcta cttcctcgcc agcaccaacc tggcgctggg
      361 ctcggggtgg gccaacacca gctccatcgc ggccgacccg agctacggtg tctacctgat
      421 ggtgctgcgc gccgacgacc ccacgccctt cgcgccacgc agcgacgaag agggcgacac
      481 gacggcggcg aagcagggga gcgacacggg cgccgtgcag gtgcgcatcg accgcgcggg
      541 aatcgagcgc cgcatcgtcg ccctgccgat gccggtgcgc cggtacaccg acctgttcgc
      601 cgggcccaag ggcaccgtct tcgtggcgga gcgggtcgag aaccagaccg gcatggtgct
      661 gcagaagttc tcgcaggccg accgcaaggc gaccgagttc gtgcgcggcg ccacccggat
      721 ctccgtctcc ccggacggag agaagatcct gttccgctcc gggacgcagt ggcgcgtcgt
      781 ggggacggcg cacacgccgg acgccggcgc cggcaacgtc gccgtgtcgc tgcgcatgta
      841 cctcgaccgc gccggggaat ggcgccagat cttcgacgag acgtggcact acgagcggga
      901 cttcttctac gaccccggga tgcacggcaa cgactggaac gcggtacgcg agcgctaccg
      961 gccgctggtt ccctgggtgc ggcatcggga cgacctcagc tacatcctcg acctcgtgaa
     1021 cggcgagctg tcggtgggcc actcgttcgt attcggcggc gacatgcccg ttgtggatac
     1081 ttcgggcgtc ggggtgctgg gcgcggatct cgtgaccgac gccggccgct ggaagatcgc
     1141 gcggatctac accttcgaga gctggaaccc cgacctggtg gcgcccctgg accggcccgg
     1201 cctgcggata cgggtcgggc agtacatctt cggcgtggac gggcgcgagc tgaccgcggc
     1261 ggacgatccc tatcgcctgc tcgacgggac cgcggggcgt cagaccgtcc tgcttgtcaa
     1321 cgaccgcccc acgatggacg ggcactggac cgtgaccgtg gagccggcgc agaacgagaa
     1381 ccagctgcgc cagcgggcct gggtggagga caaccggcgc accgtggatt cgctgtcggg
     1441 cgggcgcctg gcctacgtct ggattcccaa caccggcgga cccggcgtgg tgtcgttcaa
     1501 ccgctacttc ttcgcccagc aggaccggga aggcgcggtg atcgacgagc gcttcaacgg
     1561 cggcggcaac ctcgacgact acatggtgga ctacatgacc cgctcgctgc gggcggccat
     1621 caccaacgag gtgccgaacg gcgcgccgtt ccggctgccg gccggcatcc tggggcccaa
     1681 ggtgctgctg atcaacgagc tggcgggctc ggggggcgac tacttcccgt gggcgttccg
     1741 ccagcagcag gcgggacccc tggtcggtgc gcgcacgtgg ggcgggctgg tcaagtcgtc
     1801 ggtacactac ccgctggtgg atggcggcgc cgtcacggcg cccgacaacg cggtattcga
     1861 ccccatcaac aacaagtgga tcgcggaaaa cgagggcgtc ccgcccgacg tcgaggtgct
     1921 gctcgacgcc cgcagcgtcg cggccgggcg ggacccgcag ctggagcgcg gcgtgcagga
     1981 ggcactgcgg ctgctgcaag cgcgggggac acagcgcgtg acaccgcccc cgttctcgcg
     2041 gccctcccgg cgtccgaggt aaccgtgcga atcctccgca tcgccctcct ggtggccttc
     2101 gcgctcccgg agctggccgc cgcgcagcgg caacgcctct ccatggaccc gggctggcgc
     2161 ttcaccctcg gcgatcccgc cggcgcccag caaccggcct tcgacaatca ccagtggcgc
     2221 cggctcgacc tgccgcatga ctggagcgtc gagggcacgc cgcagcagga cgccccgagc
     2281 ggcggacgcg ggggctactt cccgacgggc ctcggctggt accgcaaggc gttccggctc
     2341 ccggccaccg cgcgcggccg cgaggtgtgg ctcgagttcg acggcgtcta catgaacagc
     2401 gacgtgtgga tcaacggcgc gcatctcggg aagcgcccct atggctactc gaccttcgcg
     2461 tacgacatca caaagcacgt cgtccagggg gtcaacgtcg tcgccgtgcg cgtggacaac
     2521 tcggcgcagc ccaactcccg ctggtacacg gggagcggga tctaccgtca tgtctggctg
     2581 accatcgtgg acccgttgca cgtggggcac tggggcgtct acgtgacgac gccgcgggtg
     2641 gactcgtcca gcgccgacgt ggtcgtacgc acgcacgtcg agaacgataa cccggccgtg
     2701 cgtcggaccg tgctgcgctc gaccgtgatc gacggttccg ggcacgaggt ggcgcacgcg
     2761 gatacatcga tttccgttgg cattggcggg agtaccgacg tcgagcagca catcgtcgtc
     2821 ccgtcaccac agttgtggtc cgttgaacgg cccacgatgt actcgttgcg caccgagtta
     2881 ctgaccgcct cgaccgcccg accgcccatc gacgttctcg cctctccgtt cggcatccgc
     2941 acgatcgcct acgacacgga caaggggttt ctgctgaacg gccggcaggt caagatgcgg
     3001 ggcgtcaacc tgcaccacga cgcgggcgcg ctgggtgcgg cggtgcccga gcgtatctgg
     3061 gagatgcgcc tggagcgcct caaggcgatg ggcgcgaacg cgatccgcac ctcgcacaac
     3121 ccgccggcac ccgagttcct ggacctgtgc gaccgcctgg gattgctcgt catggccgaa
     3181 gcgttcgacg agtggaccat cggcaaggtg ccggagggtt accacaagta cttcgcggaa
     3241 tggtccgagc gcgacgtcac cgacttcgtc catcgcgacc gcaaccaccc ctcgatcgtg
     3301 ctttggagcg ccggcaacga gatcggggag caaaccacgc ccgacggcgt tgacgtgctg
     3361 cggcgactcg tcggcatctt ccatcgcgag gacccgacgc ggccggtcac caccggcaac
     3421 gaccagatct acgccgacgg gcatcccgcc acgccggcat ttctcgaggc cgaggacatc
     3481 gtcggctaca actacgtgga ccgctggcac gagcgccgcg aactgttcgc cgagcaggac
     3541 cggcacgatc accccgactg gaagatgatc ggtacggaga gcggctcgct gttccagtcg
     3601 ctggacgagc ggtactcgct gggcgacgac tccaccgtcg tgcgacccaa ctacgtcagc
     3661 gggatgacgc aggcggagcg gctctggaag tgggtcacgc tgcacgacta cttcgcgggc
     3721 aacttcatgt ggaccgggat cgactacctg ggcgaaagct tctggccctt caagggtttc
     3781 gggtccggca ccatggacat cacagggcag cccaaggacg cctactacct ctaccagagt
     3841 ctgtggaccg accagccggt gctgcacctc ttcccgcact ggaactggac cggtcgcgag
     3901 gggcaggtga ttccggtggt ggcctactcg aactgcaaca gcgtggagct gtttctcaac
     3961 gggcgctccc tgggcgagaa gcgcatcgag tttccggcgc aggggacatc gggtggctgg
     4021 aacagctatg ccttgccggt cgtccggtcc acgaccaacg acctgcacat gagttgggac
     4081 gtcccctacg agcccggggt cctgcgggcg gtcggcaagc ggcgggacgg cacagtgggc
     4141 tgcgaggcgg aggtgcgcac ggccggtgcg ccggccgcga tccgtctgct cccggaccgg
     4201 gacaccatca cgaccggcgc gggggacgtg gcgctcgtgg cgttcgagat cgtggattcg
     4261 gccggcacgg tggtgcccac ggcggggaac ctcgtgcacg tcaccgtgac cggcggcact
     4321 ctcgtggcgc tcgacaacgc tgacatgcgg gaccacgatc cgtaccggac ggatcgtcgg
     4381 catgcgttca acgggcgggg gctcgcgctg ctgctcggcc tgcagaccgg agtaatgcgc
     4441 gtgaccgcca gcgccgacgg cctgcgtgat gggagcgtga tggtcgtcgt gcgggcgggg
     4501 aaggcggccg cggtgatcca ggcagcacga tgacgccggt cagttcaggc ggggaatgaa
     4561 gtgttctcgg ccgccctgtc gcgggatcac gttcaggacg agtgccacga acgaaaaggg
     4621 ggccgtgggc attgacccac gaacccccag ggcgtggctc tagtgcacgc cggactctcg
     4681 caagatccgg atacaaatgt ggccagcatg tcttgcaggg tcaagccagg gtgcgggtgg
     4741 tgggactcga acccacatgg gcttgcgccc cggatcttgc gagaatcctg cgtctaccag
     4801 ttgcgccaca cccgcacctg ctcgacaatt gacgtaactg gagaccacga cgcagctcgg
     4861 caagacaccg tgccgctggc g
//
LOCUS       DBNGZW010000059         4217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_755685, whole genome shotgun sequence.
ACCESSION   DBNGZW010000059 DBNGZW010000000
VERSION     DBNGZW010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_755685"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /locus_tag="AC16JC_01500"
     CDS             <1..435
                     /locus_tag="AC16JC_01500"
                     /inference="COORDINATES: protein motif:HMM:NF019127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HIR5163401.1"
                     /translation="PRGAAAPAPAPVTIDTAMVFGTLGLRGTAGGNVAQVGVVLVPTP
                     VTLHGQPLPPALAAGSPVNIQAVSPDRFVATGDFAVTATRVAPLTSALATHGITVTSV
                     HNHLIAETPALYFVHFWADGAPANVLAGLRAALDSARGGPVR"
     gene            complement(524..1309)
                     /locus_tag="AC16JC_01505"
     CDS             complement(524..1309)
                     /locus_tag="AC16JC_01505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163402.1"
                     /translation="MRRAVLVCAVSLGLAWSGLRPPAAAAQRFQGMIRFTVHDENGRT
                     TEITQYAKPGKSSFMATEAGKPGGGLIVDSTAGTLTMVDGEHKTYSVMNLVAMQQMMQ
                     GLAGMARGMQGTPRGTADAAGGPKGTITATGRSEVVAGVTCQVYAYDGTNNGRHDTGE
                     VCLAKGAGLMAGGDLMGQLPGMMGMQRQSLQQRMQSWGPLGTMLAQGYGILKGTNYED
                     GKLKGTLEVTAFQRGAPPDAAFHAPAGFTEKSMGDLMGGARRP"
     gene            1363..3147
                     /locus_tag="AC16JC_01510"
     CDS             1363..3147
                     /locus_tag="AC16JC_01510"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5163403.1"
                     /translation="MNQTPLTPFPPTTELHAAVLLAAITLGLAALCGLLYRRYRKPYL
                     GWLAVAWFLYLLRVVAIVSFLASHQWSWLYWHQVLTGWTALALLWAALVFSWQLEWRG
                     RYGLLVLFPPAWSYIAIYRLDNFLLAAGPAVLFLSLVTLWTGWTFLRYYRRVRATGAA
                     LLSAALVLWGAHHLDYPFLRARGAWSPWGYYLDILFLFATGAGILMLVQDELRRGVAA
                     LSALSGDLQPRRDGNDGLGTLLQRPLALPTVRGSAMIALEGDRARCVAGAGACDGWAD
                     APLPSRAAEAAIAAIRSGRPAFVRDWPDPRPGSGGRRFPYAALLPVWRGGAATGALLL
                     VGDARDPFTALDEGFLRALGSQIGAALENADLYRRLEARTAELARLSTGIMEQQEAER
                     RRLSLELHDETAQVLAAVKLQLGLLAERVEPELAQHVAHTVELIDAGMRSVRRVTEQL
                     RPSLLDDLGLVPALRSLAESFAERAGIAVTLDAPAALPALGRETELALFRATQEALAN
                     VARHAAASSVRVLLADGFGRLTLEIADDGRGLPADFDLARIEQAGHLGLAGMRERIGA
                     LGGTAEITGAPGAGVRVRLEIPFTATGT"
     gene            3147..3815
                     /locus_tag="AC16JC_01515"
     CDS             3147..3815
                     /locus_tag="AC16JC_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5163404.1"
                     /translation="MTRPPSAPPIRVLVADDHTVVRQGIRRVLEEDPGLLVVAEAATG
                     GEVLERAARERPDVVLLDLTMPGATGLDVVRALRERVVDARVLILSVHDDREYVLESV
                     RAGAHGYLRKDSSPSEIRQAIRAVHGCDSFFSPPVARHLAAAVQQGDDQPGALEDLTS
                     RERDVLVRIARGLTNREAAGELGISVRTVEAHRDSLMRKLRIHTVAGLTRFAMEQGLL
                     GTRS"
     gene            3881..>4217
                     /locus_tag="AC16JC_01520"
     CDS             3881..>4217
                     /locus_tag="AC16JC_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017934976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5163405.1"
                     /translation="MPELPPAEATATRSTPTMPRRSGSGPAMQPRFDGGEERRRPVRF
                     SLEGKTAVVTGAGLGIGRSIAWVLAEHCAKVMVLDLDGGPAEEAAVGIRAAGGAAHAF
                     VCDVTDEAQV"
BASE COUNT          539 a         1555 c         1460 g          663 t
ORIGIN      
        1 ccccgcggtg cggccgcccc tgccccggcg ccggtgacca tcgacaccgc gatggtgttc
       61 ggaacccttg gcctgcgggg gacggccggc ggcaacgtcg cccaggtggg tgtcgtcctc
      121 gtgccgacgc cggtcacgct gcacgggcag ccgttgccgc ccgccctcgc cgcgggcagc
      181 cccgtcaaca tccaggcggt gtcgcccgac cggttcgtgg cgacgggaga tttcgccgtc
      241 acggcaaccc gcgtcgctcc gctcaccagc gcacttgcga cacacggcat cacggtgacg
      301 agcgtgcaca atcacctcat cgcggagacg ccggccctgt acttcgtcca cttctgggcg
      361 gacggtgctc cggcgaacgt gctggcggga ctcagagcgg cgctcgactc ggcgcgtggc
      421 ggtcccgtgc gctagcgcgg tcaccttcgt catcgcctga acgcacgacg ggccacgtcg
      481 ccatgagcga cgcggcccgg tccggttccg caactcccgc cgcctaagga cgccgcgcgc
      541 cgcccatcaa gtcgcccatc gacttctccg tgaaccccgc cggtgcgtgg aaggcggcgt
      601 cgggcggcgc cccgcgctgg aacgcggtca cctccaacgt gcccttgagc ttgccgtcct
      661 cgtaattcgt ccccttgagg atcccgtacc cttgcgccag catcgtcccg agaggccccc
      721 acgactgcat gcgctgctgc agtgactgac gctgcatccc catcatcccc ggcagctgcc
      781 ccatcaggtc gcccccggcc atcagcccgg cgcccttggc gagacaaacc tcacccgtat
      841 cgtgcctccc gttgttcgtc ccgtcatagg cgtagacctg gcaagtcacg ccggccacca
      901 cctccgagcg tcccgtggcc gtgatcgtcc ctttgggccc gccggcggcg tcggccgtgc
      961 cgcgcggtgt cccctgcatc cctcgggcca ttccggccaa cccctgcatc atctgctgca
     1021 tcgccaccag gttcatgacg gagtaggtct tgtgctcgcc gtcgaccatg gtcaacgtgc
     1081 cggcggtgga atccacgatc aggccaccgc ccggctttcc ggcttccgtc gccatgaagc
     1141 tcgacttccc gggcttggcg tattgcgtga tttccgtggt gcggccgttt tcatcgtgga
     1201 ccgtaaagcg gatcatcccc tgaaaccgct gcgccgctgc cgccgggggc cggagcccgc
     1261 tccacgcgag gcccagcgag acggcacaga caagtacagc acgtcgcatt cctcgccccc
     1321 tcagttggtg gtacgactcg aacgaatata caatcctgca tcgtgaacca gacgccgttg
     1381 acgcccttcc cgcccaccac cgagctgcac gccgcggtgt tgctggcggc gatcacgctc
     1441 ggcctggccg cgctctgcgg gctgctgtac cggcgctatc gcaagcccta cctgggatgg
     1501 ctcgccgtcg cctggttcct gtacctgctc cgcgtcgtgg ccatcgtgag cttcctggcc
     1561 tcacatcagt ggtcctggct ctactggcac caggtgctga ccggctggac ggcgctcgcg
     1621 ctgctctggg cggccctcgt cttctcctgg cagctcgaat ggcgcgggcg ctatggtctg
     1681 ctcgtgctgt ttccgcccgc ctggtcgtac atcgccatct atcgactcga caacttcctc
     1741 ctggccgccg gtcccgccgt cctgttcctc agtctcgtga cgctgtggac ggggtggacc
     1801 ttcctgcgct actaccgtcg cgtgcgggcg acgggagccg cgctgctctc cgccgccctg
     1861 gtcctctggg gcgcgcatca cctcgactac ccgttcctgc gggcgcgtgg ggcgtggagc
     1921 ccgtgggggt actacctcga tatcctcttt ctgttcgcca ccggagcggg aatcctgatg
     1981 ctggtgcagg acgagctgcg ccggggtgtg gcggccctgt ccgcactctc cggcgacttg
     2041 cagccgcgcc gcgacgggaa cgacgggctc ggcacgttgc tgcagcgccc gctcgccctg
     2101 cccacggtcc ggggcagcgc catgatcgcc ctggaaggcg atcgcgctcg ctgcgtcgcc
     2161 ggggcgggcg cgtgcgacgg gtgggcggac gcacccctgc ccagccgcgc cgccgaggcc
     2221 gccatcgcgg cgatccgcag tggacggccc gccttcgtgc gcgattggcc ggatccacgg
     2281 ccgggcagtg gaggccgccg cttcccctac gccgcgctcc tgccggtgtg gcgcggtggc
     2341 gccgccaccg gcgccttgct gctggtgggc gacgcgcgtg atccgttcac cgcgctggac
     2401 gaaggcttcc tgcgggcgct gggcagccag atcggggcgg cgctcgagaa cgcggacctg
     2461 taccggcgtc tcgaggcgcg caccgcggag ctggcccggc tgtccaccgg catcatggaa
     2521 cagcaggaag ccgagcggcg acggctgtcg ctcgagctgc acgacgaaac ggcgcaggtg
     2581 ctggccgcgg tgaaactcca gctggggttg ctcgccgagc gggtcgagcc ggaactggcg
     2641 caacacgtcg cgcacaccgt cgagctgatc gacgccggga tgcgcagcgt gcgacgggtg
     2701 acggagcagc tgcgtccctc gctgctcgac gacctcggcc tggtgccggc gctgcgctcg
     2761 ctcgccgagt cgttcgcgga gcgggcgggc atcgccgtca cgctcgatgc gccggcggcg
     2821 cttccggcgc tcggccggga gaccgagctg gcgctgttcc gggccaccca ggaggcgctc
     2881 gccaacgtcg cccggcacgc cgcggccagc agcgtccgtg tgctcctcgc cgacggcttc
     2941 ggacgtctga cgctggagat cgccgacgac ggccgcgggc tgcccgccga cttcgatctc
     3001 gcgcggatcg agcaggcagg ccatctggga ctggccggca tgcgcgagcg gatcggagcg
     3061 ctgggtggca ccgcggaaat caccggggcg cccggggccg gcgtgcgcgt ccggctcgag
     3121 atccccttca ccgccacggg tacctgatga ctcgaccgcc gagcgctcct cccatccggg
     3181 tcctggtggc ggacgatcac acggtggtgc gccaggggat tcgtcgcgtg ctcgaggagg
     3241 atccgggtct cctggtggtg gccgaggcgg ccacgggcgg agaagttctg gaacgggcgg
     3301 cacgcgagcg gcccgacgtc gtgctgctcg acctcacgat gcccggtgcc acggggctcg
     3361 acgtggtccg tgcgctgcgc gagcgcgtgg tggacgcccg cgtgctcatt ctcagcgtgc
     3421 acgacgaccg ggagtacgtg ctcgagagcg tgcgagccgg cgcgcacggg tacctgcgca
     3481 aggactcgag cccctcggaa atccggcagg cgatccgcgc cgtgcacggc tgcgactcct
     3541 tcttcagccc tcccgtggcc cggcacctgg cggcggcggt gcagcagggc gacgaccagc
     3601 cgggcgccct ggaggacctc accagccgcg agcgcgacgt gctggtccgc atcgcccgcg
     3661 ggctcaccaa ccgcgaagcg gccggtgagc tcgggatcag cgtccgcacc gtcgaggcgc
     3721 atcgcgacag tctgatgcgc aagctgcgca ttcacaccgt ggcgggcctc acgcggttcg
     3781 ccatggagca ggggctgctc ggcacccgat cgtgagccgc gcgcgtcgcg cgcgcgcaac
     3841 acccctcccg ctggacggat tctcaggcac atttccccgg atgcccgagc ttccgcccgc
     3901 cgaagccacc gccacccgtt cgacgccgac catgccgcgc cgctcgggga gcggaccggc
     3961 gatgcagccg cgcttcgatg gcggcgaaga gcgccgccga ccggtgcgct tcagcctcga
     4021 aggcaagacg gccgtggtga ccggtgccgg gctcgggatc ggtcgcagca tcgcatgggt
     4081 gctggccgag cactgtgcca aggtgatggt gctcgatctg gacggtggtc ccgcggagga
     4141 ggcggcggtc ggcatccgcg ctgcgggcgg cgcggcgcac gcgttcgtgt gcgacgtgac
     4201 cgacgaggcg caggtgc
//
LOCUS       DBNGZW010000060         2833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_618858, whole genome shotgun sequence.
ACCESSION   DBNGZW010000060 DBNGZW010000000
VERSION     DBNGZW010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_618858"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="AC16JC_01525"
     CDS             complement(<1..679)
                     /locus_tag="AC16JC_01525"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5163406.1"
                     /translation="MAHVGARRALAEAGITPVHYVATSFGAVMAAALASGVTHEALTT
                     ALLQPSHEKVAVLDLIAIAKGIFADHLLKPGPLRALIEQLVPVRAFEDLATPLTVTAT
                     DLDSGAAVLFGTGEGERRAGLHEALYASCALPLYFPPAVIERRRYADGGLRAVLPLEA
                     ARTIRSDLVVAVHVGAGFDEPEPTSPTPRFPPALVRAHGAAERIMMAAQVEHAIADWP
                     DLGPRLIV"
     gene            complement(845..1330)
                     /locus_tag="AC16JC_01530"
     CDS             complement(845..1330)
                     /locus_tag="AC16JC_01530"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR5163407.1"
                     /translation="MDGRHPSESEAVMAELMMPHHANLMGNVFGGVVLSLVDRVAAVC
                     AIRHSRHQCVTVSVDKVDFKEPIHVGELVTAYGRVNFAGHTSMEVGVKLVSENVLTGE
                     KRHTNSCYVTYVALDENGQPTPVPPIVPQTPEEKRRYERASQRRASRVMDRRYDEGRG
                     E"
     gene            1495..>2833
                     /locus_tag="AC16JC_01535"
     CDS             1495..>2833
                     /locus_tag="AC16JC_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HIR5163408.1"
                     /translation="MPPHSDALLGAQLPLLSLLPFAALLAGIAVIPLFAPRWWHPNRN
                     KAIVALLVSLPVLWQLGIHLGEPGRAVLADKLADYFAFIVVIGALFAITGGIHIQGSF
                     SGTPLVNTAMLGIGAVLANLLGTTGASVLLIRPLLRANRSRRHTAHIVVFFIFVVANC
                     GGLLTPIGDPPLLLGFLRGVPFEWTLRLWPQWLVAALTLLFVFNVWDQWALDREERER
                     PGSQLEQVLQHEPLRVSGASSVAALVGVLVTIITAGRAAAAGHPWAPGAREAVVALLA
                     GVAYLGSREHRQRNEFGFAPMIEVAVLFAGIFITMAPALELLNAWSQGARTVAGLRFG
                     VAHPWQFFWATGALSSVLDNAPTYLVFAASAAGLQGLPAQGPFIGALAQDPTLARLLA
                     AIATGAVFMGANTYIGNAPNFMVKAIAEENGVKMPSFFGYMAYSGGILLPIFVG"
BASE COUNT          409 a         1045 c          908 g          471 t
ORIGIN      
        1 ccacgatcag ccgcggcccc agatcgggcc aatcggcgat cgcgtgctcg acctgtgccg
       61 ccatcatgat ccgctcggcg gcgccgtggg cccgcaccag cgcaggcgga aagcggggcg
      121 tcggcgacgt cggctccggc tcgtcgaatc cggcccccac gtgcacggcg acgaccaggt
      181 cggaccggat cgttcgggcc gcctcgagcg gcaggacggc ccgaaggccg ccgtcggcat
      241 agcgccgccg ctcgatcact gccggcggaa agtagagcgg caacgcgcac gatgcgtaca
      301 gcgcctcgtg caatcccgcc cggcgctcgc cctctcccgt cccgaacagc accgctgcgc
      361 cggaatcgag gtccgtcgcg gtgaccgtga gcggcgtcgc caggtcctcg aacgccctga
      421 ccggtaccag ttgctcgatc aacgcccgca gcggaccggg cttgagcagg tgatcggcga
      481 atatgccctt cgcgatggcg atgagatcga ggaccgcgac cttctcatgg gagggctgga
      541 gcaacgcggt cgtcagcgct tcgtgggtca ccccgctcgc gagcgccgcc gccatgaccg
      601 ccccgaagga cgtggcgacg taatgcaccg gggtgatccc cgcctctgcc agggcgcgcc
      661 gggcgccgac gtgagccacg gacttggcac cgccgccccc gaagaccgcc accaccctca
      721 tccccgccgc cccccgccaa atgcggaatg aagaatgcgg aatgcggaat tgacggagct
      781 cagctgcgag cctcggactt ctccctcaaa ttccgcattc atcattctgc attccgcatt
      841 cgtttcactc cccccgcccc tcgtcatatc gcctgtccat caccctgctc gcccgccgct
      901 gcgacgcccg ctcatagcgc cgtttctcct ccggcgtctg cggaacgatc ggcggcacgg
      961 gcgtcggctg cccgttctca tccagcgcca cataggtcac gtagcacgag ttggtgtgcc
     1021 gcttctcacc cgtgagcacg ttctccgaga cgagcttcac ccccacctcc atcgaggtgt
     1081 ggcccgcgaa gttcacgcga ccgtacgccg tcaccagctc ccccacgtgg atcggctcct
     1141 tgaagtccac cttgtccacc gacaccgtga cgcactggtg gcgcgagtgt cggatggcgc
     1201 acaccgccgc gacccggtcc accagcgaca ggaccacgcc gccgaacacg ttccccatga
     1261 ggttggcgtg gtgcggcatc atcagctccg ccatcaccgc ctcggattca ctcgggtgtc
     1321 gtccgtccat cgatcgtcca ccggttcgcg ggaagctcgc cgatcgccgt atgtacggag
     1381 cggcgatcgc gggatgggaa taatgggggc agaagccgca atgcggaatg cgcgcgccgg
     1441 ccatatcttt cgcgcgcgac cggcccacgt caccgatccg cgatccagga cccgatgcct
     1501 cctcattccg acgccctgct cggcgcgcaa ctcccgcttc tcagcctgct ccctttcgcc
     1561 gcgctgctcg cggggatcgc ggtgatcccg ctcttcgccc cccgctggtg gcacccgaat
     1621 cgcaacaagg cgatcgtggc actgctggtc tcgctccccg tcctgtggca gctgggaatc
     1681 cacctgggcg agcccggccg cgcagtgctg gcggacaagc tcgccgacta cttcgcgttc
     1741 atcgtggtga tcggcgcgct gttcgccatc acgggcggca tccacatcca gggatcgttt
     1801 tccggaacgc cgctcgtgaa cacggcgatg ctgggtatcg gcgccgtgct cgcgaacctg
     1861 ctgggcacca cgggcgcctc ggtgctgctc atccggccgc tgctccgggc caaccggtcc
     1921 cggcggcaca cagcgcacat cgtggtgttc ttcatcttcg tggtggccaa ctgcggcggg
     1981 ttgctgactc ccatcggcga cccaccccta ttgctcggct tcctgagggg cgtccccttc
     2041 gagtggacgc tccggctgtg gccgcagtgg ctcgtcgcgg cgctgacact gctcttcgtc
     2101 ttcaacgtgt gggaccagtg ggcgctcgat cgtgaagagc gggagcggcc cgggtcgcag
     2161 ctcgagcagg tactgcagca cgagccgctg cgggtgagcg gcgcgagttc tgtggccgcc
     2221 ctcgtgggcg tgctcgtcac gatcatcacc gccggccgcg ccgccgcggc gggtcacccc
     2281 tgggcaccgg gcgcccggga ggccgtcgtc gcgctcctcg ccggcgtcgc gtacctgggg
     2341 tcgcgcgagc accggcagcg caacgaattc ggcttcgcgc cgatgatcga ggtcgcggtg
     2401 ctgttcgcgg ggatcttcat caccatggcc ccggcactgg agctgctgaa cgcctggagc
     2461 cagggcgcgc ggaccgtggc gggcctccgc ttcggggtcg cccacccatg gcagttcttc
     2521 tgggcgacag gcgcgctttc gagcgtgctc gacaacgctc ccacttatct cgtgttcgcg
     2581 gccagcgcgg ccgggctgca gggcctcccc gcgcagggcc cgttcatcgg cgcactggcg
     2641 caggacccga cgctcgcccg gctcctggcc gcgatcgcca ccggggcggt gttcatgggc
     2701 gcgaatacct acatcggcaa cgcgcccaac ttcatggtga aagccatcgc cgaggaaaac
     2761 ggcgtcaaga tgccgtcgtt cttcggctac atggcctaca gcggtgggat cctgctgccg
     2821 atcttcgtgg gac
//
LOCUS       DBNGZW010000061         3307 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_550746, whole genome shotgun sequence.
ACCESSION   DBNGZW010000061 DBNGZW010000000
VERSION     DBNGZW010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3307)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3307)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3307
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_550746"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..442)
                     /locus_tag="AC16JC_01540"
     CDS             complement(143..442)
                     /locus_tag="AC16JC_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163409.1"
                     /translation="MSVAVGRIRVTRTGDIRYDDTPVTLEALAPLLRDLRSAGGVVWY
                     YREQADAEPAPEAMAVVRLVIEHRLPISISTRPDFADVVLPDGSVAPRAGPHTLS"
     gene            complement(439..1101)
                     /locus_tag="AC16JC_01545"
     CDS             complement(439..1101)
                     /locus_tag="AC16JC_01545"
                     /inference="COORDINATES: protein motif:HMM:NF018446.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HIR5163410.1"
                     /translation="MSTNWTPGPQSDPPGEAVRLPETTRLEAFSDGVFAIAITLLVLE
                     IRVPNGDVVSQHGGLWPALGSLWPSYVGYTISFVTIGIMWANHHSIFQYVRHTDRRFL
                     LVNVLFLMGIGFLPFPTAVLAEHLPDAATRVPATMFYGATLVVIAILFNAVWWSGIWK
                     GRLLGRDIAHAGVQVITERYRLGPIGYLVALGAAPLSVWLSLGIHLALALFFTRAERP
                     GA"
     gene            complement(1067..1666)
                     /locus_tag="AC16JC_01550"
     CDS             complement(1067..1666)
                     /locus_tag="AC16JC_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007421695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HIR5163411.1"
                     /translation="MPRVDAAEPLRTIPSDPVAERLLRARTIIISGEINQQLAAAVSG
                     QLLALAAESDEDITVYLNSQGGHVEAGDTIHDLLRFVRPRIRMVGTGWVASIAALIYL
                     AVPRERRFCLPNTRFLLHQPAGGAGGSASDIEIEAREILRMRDRLNRIVARETGQPLE
                     RIEEDTRRNFWLDADAALRYGIVGHVVTHVDELDTRPAV"
     gene            1830..2474
                     /locus_tag="AC16JC_01555"
     CDS             1830..2474
                     /locus_tag="AC16JC_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163412.1"
                     /translation="MKLPTRELRVAVFALLGVGLVSAVVLGRGHEKRTATIPAGTALV
                     AALEREVSTERSRVGDNVELRTVNPIRLGAGVEIPAGVLVRGTVTEAKGGGRIAGAPQ
                     LGLRFTEIEVDGDRRTISAQPFHVSGKSDAGKSAVEIGGGAVAGAILGRVVGGKGGTA
                     KGAVVGAAIGTGVAVATEGGHIVLPAGQRLRVRLEGPVTVAYRPHESQEQKSGG"
     gene            2528..>3307
                     /locus_tag="AC16JC_01560"
     CDS             2528..>3307
                     /locus_tag="AC16JC_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020618419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5163413.1"
                     /translation="MPHLLRVALKQPPADAAAFPFSVPAVRSLGPLDLSPRMTFFVGE
                     NGSGKSTLLEGIAAAARLPTVGSSDLDRDTTLDAQRSLADALQLTWGKRTHRGFFLRA
                     EDFFGFQRRLSVERAEFERDLAALDGQYKDRSGYAKGLAALPVLRSLHEMERRYGRDP
                     DARSHGQAFLNLFQQRFVPGGLYLLDEPEAPLSPQNQLGLLAVLLEMVEQDAQFVIAT
                     HSPILLAFPGAVIYSFDQVPVAPLAYGDLDHVILTRDFLNDP"
BASE COUNT          516 a         1105 c         1214 g          472 t
ORIGIN      
        1 gaagtcagcc agcgggcccc cgcttcggtg agccacgtcc aacggcagtc cggtcccgga
       61 cgcgcccgaa agcaggtctg ggcccgggcg cccgccggcg acgcgagcac gagcagcgcc
      121 agcagaccac ccggcctcac gatcagctca atgtgtgcgg accggcgcgg ggcgcgacgc
      181 tcccgtcggg caggacgacg tcggcgaagt cggggcgagt ggatatcgaa atcggcaacc
      241 ggtgctcgat caccagtcga acgacggcca tcgcttccgg ggcaggctcg gcgtccgcct
      301 gctcgcgata gtaccacacg acgccacccg ccgatcgcag gtcccgcagc agcggtgcca
      361 gcgcctccag cgtcaccggc gtgtcgtcgt accggatgtc cccggtgcgg gtgacgcgga
      421 tccggccgac cgccacgctc acgccccggg ccgttcggcg cgggtgaaga acaatgcgag
      481 cgccaggtgg atcccgaggc tgagccagac cgaaagcggt gccgcgccga gggccacgag
      541 gtagccgatc gggccgagac ggtagcgttc ggtgatcacc tgaaccccgg cgtgagcgat
      601 gtcgcggccc aacaggcggc ctttccagat ccccgaccac cagacggcgt tgaacaggat
      661 ggcgatcacc accagcgtcg ccccgtagaa catcgtcgcg gggactcggg tcgcggcatc
      721 cggcaggtgc tcggccagca cggcggtggg gaaggggaga aagccgatcc ccatcaggaa
      781 caggacgttc accaggagga agcggcgatc ggtgtggcgc acgtattgga agatgctgtg
      841 gtgattggcc cacatgatgc cgatcgtgac gaagctgatc gtgtagccca cgtaggacgg
      901 ccagagggac ccgagcgcag gccacaaccc gccgtgttgc gatacgacat ccccgttcgg
      961 cacccggatc tcgagtacga gcagcgtgat cgcgatggcg aacacgccgt cggaaaacgc
     1021 ctcgagccgt gtggtctcgg ggagccggac ggcctcgccc ggcgggtcag actgcgggcc
     1081 gggtgtccag ttcgtcgaca tgggtcacga cgtggccgac gatgccgtag cgcagcgcgg
     1141 cgtccgcatc gagccagaag ttgcgccggg tgtcctcctc gatccgctcc agcggctggc
     1201 cggtctcgcg ggcgacaatg cggttcagcc ggtcgcgcat gcgcaggatt tcgcgcgctt
     1261 cgatctcgat gtcgctcgcc gagccgcctg cccctcccgc tggttgatgc agcaggaacc
     1321 gggtgttggg caggcagaag cgccgctccc gcggcaccgc gaggtagatc agggcggcga
     1381 tgctggcgac ccagccggtg cccaccatcc ggatgcgggg acgcacgaac cgcagcaggt
     1441 cgtggatcgt gtcgcccgct tcgacgtggc cgccctgcga gttcaggtag accgtgatgt
     1501 cctcgtccga ctcggccgcg agcgccagca gctggcccga gacggcggcg gcgagctgct
     1561 ggttgatctc gccgctgatg atgatggtgc gggcgcgcag caaccgctcc gcaacgggat
     1621 cgctgggaat ggtgcgcaac ggctcggcgg cgtcgacgcg gggcatgcgg gctccaggct
     1681 ggaggtctcc ttaacgatgc ccgggccacc ccgcggagcg caacggcacg gggcgaccag
     1741 gcgcaggggc cgtagcccgg gaacactctc catggccggc ggtaggagcg gtcgtcgcgg
     1801 cgatctccgt cgcgacgaag gaggcgacca tgaaactgcc aacgcgagaa ttgcgcgtcg
     1861 cggtattcgc gctgctggga gtcgggctcg tctcggccgt cgtgctgggc cggggacacg
     1921 agaagcggac ggcgaccatt ccggcgggaa cggcgctcgt cgccgcgctc gagcgggaag
     1981 tgtccaccga gcggagccgc gtgggcgaca acgtcgagct gcgcacggtc aatccgatcc
     2041 gtctcggtgc gggggtcgag atccccgccg gcgtgctggt ccgcggcacc gtgacggagg
     2101 cgaagggcgg cgggcggatc gccggcgctc cgcagctcgg gttgcgcttc accgagatcg
     2161 aagtcgacgg cgatcgccgg acgatttcgg cgcagccgtt ccacgtgtcc gggaaaagcg
     2221 acgccggcaa gagcgccgtc gagatcggcg gcggggcggt cgccggagcg atcctggggc
     2281 gcgtcgtcgg cgggaagggc ggaacggcga agggcgcggt ggtcggcgcg gcgatcggca
     2341 ccggtgtcgc ggtggccacg gagggcgggc acatcgtcct gccggccgga cagcgcctcc
     2401 gggtccggct cgaaggcccc gtgacggtgg cgtaccggcc gcacgagagc caggaacaga
     2461 aatcgggcgg ctgacggggt cgcgcggagg ttgacgcggg gcacggggcg ggcgatcact
     2521 aagcctcgtg ccccacttgt tgcgtgtcgc cctgaagcag ccgcccgccg acgcggccgc
     2581 gtttccgttc tccgtgccgg ccgttcggtc gctcgggccg ctcgacctgt ccccccggat
     2641 gacgttcttc gtgggagaga acgggtcggg caaatccacg ttgctcgagg ggatcgccgc
     2701 cgccgcgcgg cttcccacgg tcggctcgag cgatctcgat cgggatacga cgctcgacgc
     2761 acagcgcagc ctggcggatg cgctgcaact cacctgggga aagcgcacgc accgtgggtt
     2821 cttcctgcgt gccgaggatt tcttcggttt tcagcggcga ctatcggtgg agcgggccga
     2881 gttcgagcgg gacctggcgg ccctggacgg gcagtacaag gaccgatccg gatacgccaa
     2941 aggcctcgcg gcgcttccgg tcctgaggtc gctgcatgag atggagcggc gctacggtcg
     3001 cgatcccgac gcccgctccc acggccaggc cttcctcaat ctgtttcagc agcgtttcgt
     3061 gcccggaggc ctgtacctgc tggacgagcc cgaggcgccg ctctcgccgc agaaccagct
     3121 ggggctgctc gcggtcctgc tcgagatggt ggagcaggac gcacagttcg tgatcgcgac
     3181 gcactcgccg atcctgctcg cgtttccggg cgccgtcatc tacagcttcg accaggtgcc
     3241 ggtcgccccg ctcgcctacg gcgacctgga tcacgtgatc ctgacgcggg acttcctcaa
     3301 cgacccc
//
LOCUS       DBNGZW010000062         7233 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_209962, whole genome shotgun sequence.
ACCESSION   DBNGZW010000062 DBNGZW010000000
VERSION     DBNGZW010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7233)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7233)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7233
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_209962"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1125)
                     /locus_tag="AC16JC_01565"
     CDS             complement(<1..1125)
                     /locus_tag="AC16JC_01565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent metallopeptidase FtsH/Yme1/Tma
                     family protein"
                     /protein_id="HIR5163414.1"
                     /translation="MTNRVPPPPREFQWSRMLRTLSFWAFLIVGTVLLVQLAARTRQD
                     SVEIKYSEFTAQLDQGNVKTVEITERQRIKGDLKAPIQVGRQRPADHFTTLLPFESSD
                     SWVGTLREKGVEVSAKDMRPSFGVIFLNFLPYLLMLGLILFMVRQMQQGGNRAFAFGK
                     SKAKLLSGDTPKITFADVAGCDEAKQELEEIIDFLKDPQKFQRLGGRLPKGALLIGPP
                     GTGKTLLAKAVAGEAGRPFFSMSGSDFVEMFVGVGASRVRDLFEQGKTHAPCIIFIDE
                     IDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFESNDGVILLAATNRPDILDPALLRP
                     GRFDRQIVVDMPDVKGREQILRVHMKNVPLAADVETKVIAR"
     gene            complement(1122..1676)
                     /gene="hpt"
                     /locus_tag="AC16JC_01570"
     CDS             complement(1122..1676)
                     /gene="hpt"
                     /locus_tag="AC16JC_01570"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013669898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR5163415.1"
                     /translation="MLPTAETGHQTGGAKLARIVYEAEAIERRVTELGREITAAYPDG
                     DLLVLGLLKGSFIFLSDLVRRIDRPLQVDFLVAASYGTGTESSGLVRLLYDPETVLAG
                     RHILLVEDIVDTGRTLNRLVELLRERRPRSLEICALLHKHVAEQLVLDAKFVGFDAPR
                     AFLVGYGLDHAENFRHLPYIASLT"
     gene            complement(1660..2982)
                     /gene="tilS"
                     /locus_tag="AC16JC_01575"
     CDS             complement(1660..2982)
                     /gene="tilS"
                     /locus_tag="AC16JC_01575"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR5163416.1"
                     /translation="MLTLAERFSAHVSRRRLFPRSGTALVAVSGGPDSVALLDLLTSL
                     PGGPRLVVAHVDHGIQPGSDTVAAAVRRLAARYGLPCACGSLALGARTSETAARRARY
                     AWLEEVRSRVGAQWLVTAHHRDDQVETILLRTLRGSAPAGLAGMAARTRRGVVRPLLP
                     FTRDELAAHVRERGLATHDDPANRDPRHLRSWLRHAILPQLAERLGARVRDDLTRLGK
                     AAARDRRAWDSVLDVLPGLDLQSAADSCDVARSGLRAYDDALAVAVLRAAARRMGLVL
                     GPRRARRLLDLAQRPSGRRLELGAGWHAEVAFDRLRIYRPAAPGRAVAPRAARGVAHF
                     WDYAVKWGPARAPRRMERAAWRTWILGKGWEVRGARPGDVIEPLGGVGRRPLRRILME
                     ARVPRSERPRYPVVAQGETILWVPGICRSSAALPQPGTRAVRVDVTDR"
     gene            3003..3602
                     /locus_tag="AC16JC_01580"
     CDS             3003..3602
                     /locus_tag="AC16JC_01580"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR5163417.1"
                     /translation="MVGPDDTYQLLRNLTTPVVAITSEHGGQRNGMIIDSAIRASIVP
                     TIPRLAMFIHKFNDSHDLIFASGRFVLHMLRQDQFALIRRLGFVHRRERDKLAEVPHR
                     LGRLGAPVLDGCYAHFECRVVNAMDTGSSTCFLGDVVEVGFGAAAEPRGEVMTAAYFR
                     ANVPDEWRDEYLRLLKVAQTAGLELSRNIQPLVWRELKD"
     gene            complement(3671..>7233)
                     /locus_tag="AC16JC_01585"
     CDS             complement(3671..>7233)
                     /locus_tag="AC16JC_01585"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR5163418.1"
                     /translation="LLDADRQIIHRAVGLPEPIAGRRETPLSHSLCRTLVTLGRALAV
                     GDARADDRLREHPAVTELGIAACAASPLVTADGHVVGTLAVFDFTPRSWRPDEIALLD
                     QLAATVWAHIELLRARRELEDQAAVLRRASALRAAGRPGEPTPAVPLDATRSDVWTAL
                     LDLLPDPVYLKDADCRFTRINRAHAAVLGLDDPALAVGKSDADFFTPEHAAAALADEH
                     EVMASGRPIVAKIERVQDLGGRVRWFTTSRMPVRDAGGAVIGTLGCSHDITGVMEADR
                     EERRALSLHRATLESTADGILVVDREGRIASFNRKFVALWRLPDALLASRQDERVIEA
                     MVDQVRDADGFLHRVRELYADMEASSFDLIECTDGRVFERYSQPQRIGGEVVGRVWSF
                     RDISERRRAEQQNRLLAQTIQSTGDIVSITDLEGRFTFVNRAFLAAYGYTEAEVLGRT
                     TALIDSPRNPPGLQGEIREATRTGTWRGELWNRKKDGSDIPVELATSQIRDSEGRVVG
                     LVGVWNDITLRRRAEAVQQAVYRIAEAASASDGLEGLLRAVHGIVGELMPAANFFVAL
                     YDRTAGLLSFPYFVDQVDTADPPRPAARGLTEYVLRTGRPLLGTPELVAGLERRGEVD
                     LVGAPSVDWLGVPLKVASDVIGVLAVQSYTEGVRFGAGELEILELLSAQVALAIERTR
                     SAERVQASERRYRQLFEGNPAAMFVYDAETLRFLAVNEAAVRRYGWSREEFLTMTLTD
                     MRPEEEAAKLREILAQYRGGTARATGLRHQRRDETLLDVEVTYDDIDFAGRHARLAVV
                     EDVTERRRIEDRLRQAQRMEAVGQLAGGIAHDFNNLLTAIVGYATLLDRALPPADELR
                     EEVHEIIGAARRAGNLTQQLLAFSRKQVLRPTVLDVNVVVRDIERILHRIIGEHIALT
                     TALEPRLRPVRADASQLEQVIMNLAVNARDAMAGGGRITLATTNAVLDPELAREHPDV
                     RPGAWVRLTVSDTGEGMTPEVKAHLFEPFFTTKEVGKGTGLGLATVYGIVHQGGGFIT
                     VDSEPDRGTRFDIFLPAAEAPEQEATPAVAEPAATGSGTVLVVEDEAGVRRLAREVLS
                     RYGYRVLEAGDGNEALRVAGAHEGPIDLLLTDVVMPGMSGAELAERFHALRPEAGVLY
                     ASGYADEAVMRHGIPEDVPFLQKPFEPDELVRRVRELVRG"
BASE COUNT         1058 a         2643 c         2482 g         1050 t
ORIGIN      
        1 gcgcgcaatc accttggtct cgacgtcggc ggccagcggc acgttcttca tgtgcacgcg
       61 caggatctgc tcgcgcccct tcacgtccgg catgtccacg acgatctggc ggtcgaagcg
      121 acccgggcgc aacagcgccg ggtcgagtat atcggggcgg ttggtggcgg ccagcaggat
      181 gacgccgtcg ttcgactcga acccgtccat ctcgacgagc agctggttca gcgtctgctc
      241 gcgctcgtcg tggccgccgc ccagcccggc gccgcgatgg cgccccaccg cgtcgatctc
      301 gtcgatgaag atgatgcagg gcgcgtgcgt cttcccctgc tcgaacagat cgcgcacccg
      361 cgaggcgccc acgcccacga acatctcgac gaagtccgag cccgacatcg agaagaaggg
      421 ccggccagct tccccggcca ccgccttggc cagcagcgtc ttgccggtgc cgggcgggcc
      481 gatgagcagc gcgcccttgg gcagccggcc gccgagccgc tggaacttct gcgggtcctt
      541 caggaaatcg atgatctcct cgagctcctg cttcgcctcg tcgcatcccg ccacgtcggc
      601 gaaggtgatc ttcggcgtgt cacccgagag cagcttggcc ttggacttcc cgaacgcgaa
      661 tgcgcggttc cccccctgct gcatctggcg gaccatgaac aggatcagtc cgagcatcag
      721 caggtagggg agaaaattca ggaagatgac gccgaaggac ggccgcatgt ccttggcgct
      781 cacctccacg cccttctccc gcagcgtgcc gacccaggag tcgctcgatt cgaacggcag
      841 cagggtcgtg aagtgatcgg ccgggcgctg ccgccccacc tggatcgggg ccttcaggtc
      901 gcccttgatc cgttgccgct cggtgatctc caccgtcttc acgttgccct ggtccagctg
      961 cgccgtgaac tcggagtact tgatctcgac agagtcctgc cgcgtgcggg ccgcgagctg
     1021 cacgagcagc acggtgccca cgatgaggaa cgcccagaac gagagcgtcc gcagcatgcg
     1081 gctccattgg aattcccgcg gaggcggggg cacgcgattc gtcatgtcag actggcaata
     1141 taagggaggt gccggaaatt ctcagcatgg tcgagcccgt accccaccaa aaaggcccgc
     1201 ggggcgtcga atccgacgaa tttggcgtcc agcaccagtt gttccgccac gtgtttgtgc
     1261 agcagagcgc aaatctccag actgcggggc cggcgctccc gcaacagctc caccaggcgg
     1321 ttcagcgtcc ggcccgtgtc cacgatgtcc tccaccagca gaatgtgccg tccggcgagc
     1381 accgtttcgg gatcgtacaa cagccgcacc agaccgctcg actcggtccc cgtcccgtag
     1441 ctcgccgcca ccagaaaatc cacctgcagc gggcggtcga tccgccgcac caggtcgctc
     1501 agaaagatga agctcccttt caggaggccg agcaccagca ggtcgccgtc cgggtacgcc
     1561 gcggtgatct cgcgccccag ctcggtcacg cggcgctcga tcgcctctgc ttcgtacacg
     1621 atgcgggcca gtttggctcc gccggtctga tggccagtct cagcggtcgg taacatcaac
     1681 cctcacggcc cgggttcccg gctggggcaa cgccgcgctg ctgcggcaga tgccgggcac
     1741 ccagagaatg gtttcgccct gcgccaccac cggatagcgg ggacgctcgc tgcgcggtac
     1801 gcgcgcttcc atcagaatcc gccgcaaggg acggcggccc acgccgccca gcggctcgat
     1861 cacgtcgccg ggacgggcgc cgcgcacctc ccatcccttt cccagaatcc aggtgcgcca
     1921 ggcggctcgc tccatccgcc gcggggccct ggcgggtccc cacttcaccg cgtagtccca
     1981 gaagtgcgcg acaccccggg cggcgcgcgg cgcgaccgct cgccccgggg ccgccggccg
     2041 gtagatccgc aggcggtcga aggccacctc ggcgtgccag ccggctccca attccagccg
     2101 ccggccggag ggccgctgcg ccagatcgag gaggcgccgg gcgcggcgtg gcccgagcac
     2161 gagccccatg cgccgcgcgg ccgcgcgcag cacggcgacc gccagggcgt catcatacgc
     2221 ccgcagcccg gagcgggcaa cgtcgcacga gtccgccgct gattgcagat ccaggcccgg
     2281 caacacgtcg agcaccgagt cccatgcacg acgatcccga gccgccgcct tccccagccg
     2341 ggtcaggtcg tcgcgcacgc gggcgccgag gcgctccgcg agctgcggaa gaatggcatg
     2401 gcgcaaccag gagcgcaggt gtcgcggatc acggttggcg gggtcgtcgt gcgtcgcgag
     2461 cccacgctcc cggacgtgcg ccgcgagctc gtcacgcgtg aacgggagca gcgggcgcac
     2521 gacgccgcgg cgcgtgcgcg ccgccatccc cgcgagcccg gcgggcgcgc tgccgcggag
     2581 cgtccgcagc aggatcgtct ccacctggtc gtcacggtgg tgcgccgtga cgagccattg
     2641 ggcgccgacg cgcgacctga cttcctcgag ccaggcgtag cgggcgcggc gcgcggctgt
     2701 ctcgctggtc cgagcgccca gcgccagcga tccgcacgcg cagggcagcc cgtagcgcgc
     2761 ggccaaccgc cgcactgcgg ccgcgacggt gtcgctcccc ggctggatgc cgtggtccac
     2821 atgggccacg accagccgtg gtccgccggg gagggacgtg agcaggtcga gcagggcgac
     2881 cgagtcgggt ccccccgaca ccgccaccag ggcggtaccg gaccgcggga acaggcggcg
     2941 gcgcgagacg tgggcgctga agcgctcagc gagggtgagc atggggcctt accttactat
     3001 acatggtcgg acccgatgac acctaccagc tgctgcgcaa cctcaccacc ccggtcgtcg
     3061 cgatcacctc ggagcacggg ggccagcgca acgggatgat catcgacagc gcgattcgcg
     3121 cttcgatcgt ccccacgatc ccgcgcctcg cgatgttcat ccacaagttc aacgacagtc
     3181 acgacttgat cttcgcgagc gggaggttcg tgctgcacat gctgcgccag gaccagttcg
     3241 cgctgatccg ccggctcggc ttcgtgcacc gccgcgagcg cgacaagctg gcggaggtcc
     3301 ctcaccggtt gggacgtctc ggggcccccg tgctggacgg gtgttacgcc cacttcgagt
     3361 gccgcgtcgt gaacgccatg gatacgggca gctcgacgtg cttcctgggc gacgtggtgg
     3421 aggtgggatt cggcgccgcc gccgagccgc ggggcgaggt gatgaccgcc gcctacttcc
     3481 gcgccaacgt gccggacgag tggcgcgatg agtatttgcg gctgctgaag gtggcgcaaa
     3541 cggccgggct ggagctgtcg agaaacatcc agccactggt ctggcgggaa ctgaaagact
     3601 gaggggccag gtgtcaggtg ccgggtgcca gggtctcctc ccctgacccc tagcacctgg
     3661 cacccggcac ctagcctctc accagctccc gcacccgacg caccagctcg tccggctcga
     3721 acggtttctg caggaacggc acgtcctccg ggatgccgtg gcgcatcacg gcttcgtcgg
     3781 cataacccga cgcgtacagc accccggcct cggggcgcag ggcgtgaaag cgctccgcca
     3841 gctcggcgcc gctcatcccc ggcatgacga cgtcggtgag cagcaggtcg atcggtccct
     3901 cgtgcgcgcc cgcgacccgc agcgcttcgt tcccgtcgcc cgcctccagc acccggtagc
     3961 cgtaacggct gagcacctcg cgcgcgaggc ggcgcacgcc cgcttcgtcc tccactacca
     4021 acaccgtgcc actgcccgtc gctgccggtt ccgcgaccgc gggcgtggcc tcctgctcgg
     4081 gcgcttcggc ggccgggaga aagatgtcga accgggttcc gcgatcgggc tcggagtcga
     4141 ccgtgatgaa tccgccgccc tggtgcacga tgccatacac ggtggcgagg cccaggccgg
     4201 tgcccttccc cacctccttg gtcgtgaaga acggctcgaa caggtgcgcc ttcacctcgg
     4261 gcgtcattcc ctctccggta tcggagaccg tcagccgcac ccaggcaccc ggccggacat
     4321 cgggatgctc ccgcgcgagc tcggggtcga gcacggcatt ggtggtcgcg agggtgatcc
     4381 ggccgccccc ggccatcgcg tcgcgtgcat tcacggccag gttcatgatc acctgctcga
     4441 gctggctcgc gtcggcgcgg accggccgca gacggggctc gagtgcggtg gtgagcgcga
     4501 tgtgctcccc gatgatgcga tgcaggatgc gctcgatgtc gcgcacgacc acgttgacgt
     4561 cgagcacggt gggccggagc acctgcttgc ggctgaacgc cagcagctgc tgcgtcaggt
     4621 tgccggcgcg ccgcgccgcg ccgatgatct cgtggacctc ctcgcgcagc tcgtccgcgg
     4681 gcggcagcgc ccggtccagc agggtggcat agcccacgat ggccgtgagc agattgttga
     4741 agtcgtgggc gatgccgccg gcgagctgcc cgaccgcttc catcctctgt gcctgccgca
     4801 gccggtcctc gatgcgccgc cgctcggtca cgtcctcgac caccgcgagc cgcgcatgcc
     4861 gcccggcgaa gtcgatgtcg tcgtaggtga cctcgacgtc gagcagcgtc tcgtcgcgcc
     4921 gctggtggcg cagcccggtg gcccgggccg tgccgccgcg gtactgcgcc aggatctccc
     4981 gcagcttggc cgcctcttcc tccggccgca tgtcggtcag ggtcatcgtg aggaactcct
     5041 cgcgcgacca gccgtagcgg cgcacggccg cctcgttcac cgccaggaag cggagcgtct
     5101 cggcatcgta cacgaacatc gccgccggat tgccctcgaa cagctgccgg tagcggcgct
     5161 cgctcgcctg cacccgctcg gccgaccggg tgcgctcgat cgccagcgcg acctgcgccg
     5221 acaagagctc caggatctcg agctccccgg cgccgaagcg cacgccctcg gtgtagctct
     5281 gcaccgccag cacgccgatc acgtcgctcg cgaccttgag cggcacgccg agccagtcca
     5341 ccgagggcgc gcccaccagg tccacttcac cgcgccgctc cagcccagcc acgagctccg
     5401 gcgtgccgag cagcggccgc ccggtccgga gcacgtactc ggtgagtccc cgcgccgccg
     5461 gccggggcgg atcggccgtg tccacctggt ccacgaaata aggaaaggaa aggagcccgg
     5521 cggtccggtc gtagagcgcc acgaagaagt tggcggccgg catcagctcg cccacgatgc
     5581 cgtgcaccgc gcgcagcagg ccctcgagcc cgtcggacgc gctggcggct tcggcgatgc
     5641 ggtacaccgc ctgctgcacg gcctcggcgc ggcgccgcag ggtgatgtcg ttccagacgc
     5701 cgacgaggcc caccacccgg ccctcggaat cgcggatctg cgaggtggcc agctccacgg
     5761 ggatgtcgct gccgtccttc ttccggttcc acagctcccc gcgccaggtg ccggtccgcg
     5821 tggcctcgcg gatctcgccc tggaggcccg ggggattgcg cggtgagtcg atgagggcgg
     5881 tggtccggcc cagcacctcg gcctcggtgt agccgtacgc cgcgaggaag gcgcggttca
     5941 cgaacgtgaa ccggccctcg agatcggtga tgctcacgat gtcgccggtg ctctggatgg
     6001 tctgggccag gagccggttc tgctgctcgg cacggcggcg ctcgctgata tcgcggaagc
     6061 tccagacgcg ccccacgacc tcgccgccga tgcgctgcgg ctgggagtag cgctcgaaca
     6121 cccgtccgtc ggtgcactcg atgagatcga agctcgacgc ctccatgtcg gcatacagct
     6181 cgcgcacccg gtgcaggaag ccgtcggcat cccgcacctg atcgaccatg gcctcgatca
     6241 cccgctcgtc ctgccggctc gccaggaggg cgtccggcag ccgccacagc gccacgaact
     6301 tccggttaaa gctcgcgatg cgcccctcgc ggtccaccac gaggatgccg tccgccgtgg
     6361 actcgagggt cgcgcggtgc agcgacaggg cgcgccgctc ctcccggtcc gcttccatca
     6421 cgccggtgat gtcgtgcgag cagcccagcg tgccgatcac ggcaccgccc gcgtcgcgca
     6481 ccggcatgcg gctcgtcgtg aaccagcgca cccggccgcc caggtcctgc acccgctcga
     6541 tcttcgcgac gatcgggcgg ccgctcgcca tgacctcgtg ctcgtccgcc agcgcggccg
     6601 cggcgtgttc cggcgtgaag aagtccgcgt cgctcttccc cacggcgagc gccggatcgt
     6661 ccaggccgag caccgcggcg tgcgcgcggt tgatccgcgt gaagcggcag tcggcgtcct
     6721 tcaggtacac gggatcgggg agcaggtcga gcagcgccgt ccacacgtcg ctgcgtgtcg
     6781 cgtcgagcgg cacggcgggc gtgggttccc cgggccgccc ggcggcgcgg agcgccgagg
     6841 cgcggcgtaa cacggcggcc tggtcctcga gctcgcgacg cgcccgcagc agctcgatgt
     6901 gcgcccatac ggtcgcggcg agctgatcga gcaatgcgat ttcgtcgggg cgccagctgc
     6961 gcggcgtgaa gtcgaagacg gccagcgtgc cgacgacgtg gccgtcggcg gtgacgagcg
     7021 ggctcgcggc gcaggcggcg atgcccagct cggtcacggc gggatgctcg cgcaggcggt
     7081 cgtcggcacg ggcgtcgccc acggcgagcg cgcggccgag tgtcaccagc gtgcggcaga
     7141 gggagtggga cagcggcgtc tcgcgccgtc cggcgatcgg ctcggggagt ccgacggcgc
     7201 ggtggatgat ctggcggtcg gcgtcgagca ggc
//
LOCUS       DBNGZW010000063         3848 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1776329, whole genome shotgun sequence.
ACCESSION   DBNGZW010000063 DBNGZW010000000
VERSION     DBNGZW010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1776329"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..575
                     /locus_tag="AC16JC_01590"
     CDS             3..575
                     /locus_tag="AC16JC_01590"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR5163419.1"
                     /translation="MQSRASRATEARRLEVAAGQEPFAIVLGYSDSRVPAEIVFDQGL
                     GDLFVIRVAGNIVAASQVGSVEFAAARYGTRLVVVLGHSQCGAVLATLEELQQPRANQ
                     SPNLRSIVDRVRPSVEGLLATELRHDPAALLHQSVRANIRASADHLRHGSAVLERLIA
                     RDGLLVVGAEYSLETGSVCFFDGVPEEESA"
     gene            761..1276
                     /locus_tag="AC16JC_01595"
     CDS             761..1276
                     /locus_tag="AC16JC_01595"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR5163420.1"
                     /translation="MRNLLAVLFPALTLAFAGCAAMPPSTPAGGTDVVERNKALVREL
                     TEVVWNQRGLDRIPDFYGPDFVGDYRPYALRQGHEGIRASVADAWTAFPDYHEELLDL
                     IGEGDRVVVHLAISGTQRGQWGPLPPTGKRVQFEEIVILQIRDGKVRRQRGIVDNLNA
                     LRQLGVVPTPR"
     gene            1515..1784
                     /locus_tag="AC16JC_01600"
     CDS             1515..1784
                     /locus_tag="AC16JC_01600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019890153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163421.1"
                     /translation="MDEDLEAMTREQLAAEARRLRAGIRAHRDSTGHALCWHHPALWG
                     LLPEKTDPLPTVPTWPEFLRGCLQYRESLDKQLPAAPRSNAPYRP"
     gene            1916..2122
                     /locus_tag="AC16JC_01605"
     CDS             1916..2122
                     /locus_tag="AC16JC_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163422.1"
                     /translation="MRNRVLGVVGVLWGGLILLGRLAGCGAGDGAGAYGAGQAVGLLF
                     AVLLLVVGLYYLVRGGGSARPKSP"
     gene            2421..2813
                     /locus_tag="AC16JC_01610"
     CDS             2421..2813
                     /locus_tag="AC16JC_01610"
                     /inference="COORDINATES: protein motif:HMM:NF019932.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5163423.1"
                     /translation="MGIHAAAERFLRALDNLDWEPFRASWTSTPTVFFPFGDTPERVT
                     GQVAVEGRRRRFFEEARARRPGPPYLDLKPLDIQVERYGDAGLVTFTLQLTVGGRRLP
                     LQRRTLLFVRERDAWKLAHMHASGASQP"
     gene            2907..3335
                     /locus_tag="AC16JC_01615"
     CDS             2907..3335
                     /locus_tag="AC16JC_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010221778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LIC_13387 family protein"
                     /protein_id="HIR5163424.1"
                     /translation="MLSTLAPLLIAGSAAIVMLFGLLHLLYTFHGPKLLPRDHDLEAR
                     MQEVSPVITRETTMWECWVGFNASHSYGLILFGAVYGYLSLAHSAFLFRSMFLLSLGL
                     LLLGGYAFLAKRYWFRVPFRGILLATVLYVIALIIDWAQI"
     gene            3347..>3848
                     /locus_tag="AC16JC_01620"
     CDS             3347..>3848
                     /locus_tag="AC16JC_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163425.1"
                     /translation="MMELSDPRWTNLAGGRRLPFDPRPALRRLEDGVDVSGAWDELWE
                     GLHHQGDVGEASYACVPHLVRIHAARGVPDWNTYALVATIDLGRDRRHNPQVPSWLDA
                     EYVSAINHLAELVLREFPRATDQAAVRSMLAVLAIWKSARTHGRALVELSEDELLELD
                     AGLGGDL"
BASE COUNT          612 a         1217 c         1331 g          688 t
ORIGIN      
        1 aggtgcagag ccgcgcttcc cgcgcgaccg aggcccgccg gctcgaggtg gcggccgggc
       61 aggagccgtt cgccatcgtc ctgggctact ccgactcccg ggtgccggcg gagatcgtct
      121 tcgaccaggg gctgggcgac ctgttcgtga tccgcgtggc cggcaacatc gtggcggcct
      181 cacaggtcgg cagcgtggag ttcgcggcgg cgcggtacgg cacgcggctg gtcgtggtgc
      241 tcggccactc tcagtgcggc gcggtgctgg ccacgctgga ggagctgcag cagccgcggg
      301 ccaaccaatc cccgaacctg cgctcgatcg tggaccgcgt gcgtccctcg gtggagggcc
      361 tgctggccac cgagctgcgg cacgacccgg ccgcgctcct gcaccagtcc gtgcgggcca
      421 acatccgcgc ctccgccgat cacctgcggc acggctcggc ggtgctggag cggctgatcg
      481 cgcgcgacgg cctgctggtg gtgggcgcgg agtactcgct cgagacgggg agcgtgtgct
      541 tcttcgacgg ggtgccggag gaggagagtg cgtgaagagg gaccgggcat cggagcgcac
      601 gatgaagaca ttctcactcc actggcgcag cggggtcctg gaagaggaag cccagatcga
      661 gacgccgtac ctacggccac tccggccgca gatctggtga gcgagcagcg acttcgccac
      721 ggggcgccgg cgtggcgcca gattccagga ggaaggatcg atgcgcaacc ttctggccgt
      781 cctgtttcca gccctcacgc ttgccttcgc cggctgtgcc gccatgcctc ctagcacgcc
      841 ggcgggaggc accgacgtcg tcgagcgcaa caaggcactc gtgcgggagc tgaccgaggt
      901 cgtctggaac caacgcggcc ttgatcgtat cccggacttc tacgggcccg atttcgtcgg
      961 ggactatcgc ccatacgcgt tgcgccaggg acacgagggt atccgcgcca gtgtggcaga
     1021 cgcctggacg gctttccccg attaccacga agagctcctt gacctcatcg gcgagggtga
     1081 tcgtgtcgtc gtgcacttgg cgatctcggg aacgcaacgc ggtcagtggg ggcccctgcc
     1141 acccacggga aagcgcgtac agttcgaaga gatcgtcatt ctccagattc gtgacggcaa
     1201 ggtgcgccgg cagcgcggca tcgtcgacaa cctcaacgcg ctgaggcaac tcggcgtggt
     1261 gccgacgcca cgctagtgac cggataccca ctcgcgcgat gtccgccgaa caacaggctg
     1321 gagcgggccg gctcgtcgcc ggcggctcag cctgaccgtc atgccgattg actgcgaatc
     1381 gatgtcgcta gcgtctggcg agggcgggcg ttggccggcg caggccgagg aattgtcagc
     1441 gcaccgctca actcaaacgt tcggcggaca agacgtatca ccgaccgctc cccgaggcat
     1501 cgcaaggagc tatcatggac gaggacctcg aagcaatgac gcgcgagcag ctagccgccg
     1561 aggcgcggcg gctacgcgcg gggattcggg cgcaccggga cagcacggga cacgcgctct
     1621 gctggcatca cccggcactc tggggtctcc tccccgagaa gactgatccg ctgccgacgg
     1681 tgcccacgtg gccggagttt ctccggggct gtctgcagta ccgcgagtct ctggataagc
     1741 agctgcctgc tgcgcctcgt tcaaatgcac cgtatcggcc gtgaccgcgg ggcgacgagc
     1801 agcggatacg agagccgccg aacctctcat tggatcggac cgccggctcg cgtacgctcg
     1861 ccgcggccgc tcaacgcgac gtgagacggt ccttgcatga ttgaggaggt gcagagtgcg
     1921 taacagggtc ctcggcgtgg tcggcgtact ttggggcggg ctgattctct tggggcgtct
     1981 tgccggttgt ggtgccggtg atggagccgg ggcctacggg gccgggcagg cggttgggtt
     2041 gctgttcgcg gtattgctgc tggtggtggg gctgtactac ctcgtacgcg gcggcggctc
     2101 ggcgcgtccg aagtcaccgt gagtccgcgg ggcgtccggc tacggatcta ggcggtgggg
     2161 cgccgcgact cgatgcgtgg aatggcgcgg gccgccgaac ctcacgcttc agcggaccgc
     2221 agctcgcgct gctcgccccg gccgccgagc gcgcgcgtta ggcggaccca tcacgatgcc
     2281 aaaggaggtt tatgcgctac acaaagtcgc ggcgggcatt ttcatatatg gccgtcgtcg
     2341 tgctcctgcc aggcgcagcg gcatccgcgt tggcgcagac gtcggtgagc gcctcacagg
     2401 ccacgatatc ggaggatctc gtgggaattc acgcggcggc tgagcggttt ctgcgtgcgc
     2461 tcgacaatct cgactgggag ccattccggg cgagctggac gtcaacgccg acggttttct
     2521 tcccgtttgg cgatacgccg gagcgcgtta ccgggcaggt agcggtcgag gggcgccggc
     2581 ggcggttctt cgaggaagcg cgggcacgca gacccgggcc gccgtatcta gacctgaagc
     2641 cgctcgacat ccaggtcgag cgctacgggg atgcgggact cgtcacattc acgcttcagc
     2701 taaccgtcgg cgggcgccgg ttgcccctgc agcggcgtac gctcctgttc gtccgagagc
     2761 gcgacgcttg gaagctggcc catatgcatg cgtctggggc gagtcagcca taaggtgctg
     2821 gcgcgagctc gcataacacc gcgttgcggc ggatactctg caagcagcgc gccgctgaac
     2881 gcgaccgtta ggcctaggga ggtcgcatgt tgagcacact tgcaccattg ctcattgctg
     2941 gcagcgcagc gatcgtcatg ttgttcggcc tcctccacct tctgtatacc ttccacggcc
     3001 ccaagctgct gccacgcgac cacgatctag aggcgcgcat gcaggaggtg tcgcctgtga
     3061 tcacgcgcga aacaacaatg tgggaatgct gggtcggctt caatgccagt cacagctatg
     3121 gcctaatcct cttcggtgcg gtgtacggct acctgtcctt ggcgcacagt gccttcttgt
     3181 tccggtccat gtttcttctg tcgctcgggc tgttgctgct tggcgggtat gccttcttag
     3241 ccaaacgcta ctggttcagg gttccgtttc gcggcatcct tctggcaacc gtcctctatg
     3301 tcatcgcgct catcatcgac tgggcccaaa tctagttatc cggacagtga tggaactcag
     3361 cgatcctcgc tggactaacc tggccggcgg ccggcgcctg ccgtttgacc cgcggcctgc
     3421 cttacgccga cttgaagacg gggtggatgt gtcaggcgcg tgggacgaac tatgggaagg
     3481 gctccatcac caaggcgatg tcggggaggc atcctacgcc tgcgtaccgc atctggtgcg
     3541 gattcacgca gcccggggag ttccggattg gaacacctat gcgctcgtgg caacgatcga
     3601 tctgggtcgt gaccggcgcc acaatccgca agtcccgtcc tggctggacg cagagtacgt
     3661 cagtgcgatc aaccacctgg ccgaactggt cctgcgggaa ttcccgcgcg cgacggatca
     3721 agcggcagtt cggtctatgc tggcggtgct tgccatctgg aagagtgcga gaacgcacgg
     3781 gcgggctctg gtcgaactgt ctgaagacga gctgctcgaa ctggacgctg ggctgggcgg
     3841 cgatctga
//
LOCUS       DBNGZW010000064         4639 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1026428, whole genome shotgun sequence.
ACCESSION   DBNGZW010000064 DBNGZW010000000
VERSION     DBNGZW010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1026428"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="AC16JC_01625"
     CDS             <1..854
                     /locus_tag="AC16JC_01625"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR5163426.1"
                     /translation="PTAHRTGCAGARAVSVALAFESVSFDYAGRPALTNVSFAIAPGE
                     RVALLGRNGAGKSTLLRLVTGLIHPAIGTIRVGDWDAASRRPDELARRVGSLFQHADQ
                     QLFARTVADDVAFGPRVTGIPATETAERVRRALESLGLLEVAAEHPYDLPAPWRKLAA
                     LAGALALEPMLLVLDEPTAGLDRRSRARVGQALAARADGGTALLVVTHDLGFAAETLQ
                     RALVLEQGGLARDVTLGPFLAEAKGLEGLGLVAPPLARLSRALGLPGGPVREIEVASA
                     LWQRCSA"
     gene            883..2775
                     /locus_tag="AC16JC_01630"
     CDS             883..2775
                     /locus_tag="AC16JC_01630"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR5163427.1"
                     /translation="MVNPVVFATHLARAVDLFRDPAKKEDQKREFRILVDLLKESGVA
                     ARVVDDVLQVNGEAVTAPEFQPLVQRMVLHGVTEIMIPADAPVAHLFELLRALADQPG
                     SSLNIVSRLRASGASRVSVVLAALDEPEDVPPGRSEPEAPEEPEGPPETADMGTRGVL
                     RGEPVRDLKSTPIAGAEGVEQVEQNLAPPSVALPSPDAGTPHGDVFPHETEKQPELPS
                     THVEGVAPPPQESVPPLPANLPPRPPPPPPPPLTQAPTFRPSSAAIMPPPLSPQPTEA
                     VEPAPQSEIAREAASPTFALREALSKDTTDLLASLEHDPQAASVGETLTVLTRQVESA
                     MRQGKVEAAIQIVYSVVRAERTIEDAAVRRQYGIALRRMITRTMLDSLSKLVLVPHLE
                     DAAAMVLQRAGPDGMEVLLELLSTSNTVMERRGVFNALIQMKEGQDQLVHMLGHPQWF
                     VVRNVADLVGELGLEAAVPALAKQLDHPDERVRKQVALALAKIGTRGAAEPLRRVLRD
                     RSSEVRRQAALGVGGRKASALAMPLVVALEEEKDPEVVRELVLALGRIGSPDAVQALI
                     KIAQPSGRLFGRKPSALRVTAVEALRVAGTPTAIGTLQNLSNDSDKQVRASAREALSE
                     LSLKKS"
     gene            complement(2778..3848)
                     /locus_tag="AC16JC_01635"
     CDS             complement(2778..3848)
                     /locus_tag="AC16JC_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HIR5163428.1"
                     /translation="MTASSHDFLKRLLDSPGPSGFEAAPARVWRDEVKRFADELRPDV
                     HGNSVGTVNPKGKPRLMFAGHIDEIGLQITHIDEDGFLYFAGIGGWDSQVLVGQRVIV
                     VGPAGPVRGVIGKKPPHLMKKEDFDKVSQITDLWIDIGAASRDAALARVHVGDPAVLD
                     AASQEFPGGRIVSRSIDNRIGAYVVAEALRLLAQQRPPHAAVFAVATTREEIAYTGGG
                     ARTSAVGIDPQVALVVDVTHSTDWPGADKKQGGDHKLGGGPVFGRGSALNPVVFDFLV
                     QCAVEEGIPYSVQAAPRDTGTDADAIHNALRGIPTGLVSVPNRYMHSPNEMVALADVE
                     RAARLLAAFARKLGPDAGFIPT"
     gene            complement(3845..>4639)
                     /locus_tag="AC16JC_01640"
     CDS             complement(3845..>4639)
                     /locus_tag="AC16JC_01640"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR5163429.1"
                     /translation="SPIAPVGQPFVHTGSPVFSAPPVAADALVRAGFDVVSTANNHAW
                     DTGLTGVLETVKQLDRVGLPHTGTGRTLADAHRPAIVERKGWRVAFFAVTRAYNPAPR
                     RFYSHVGSRYIAYADSAWLYPAIRRIKASGAADFVVVSIHAGTELSDGPDDALRAFLE
                     GAVDAGADLCLGHHPHVLQPVEWYRGKPIAYSMGNFIFKRGNPLTAYSGVFTFMITPD
                     GFVMADVIPVRSGFQATFAARTAADTVRRRVRLVGALPIILFATLP"
BASE COUNT          683 a         1589 c         1677 g          690 t
ORIGIN      
        1 cccccaccgc tcaccgtacc ggatgcgctg gagcgcgtgc cgtgagtgtg gcgctcgcgt
       61 tcgaaagcgt ctcattcgac tacgccggac ggccggcgct taccaacgtc tccttcgcga
      121 ttgcgccggg tgagcgcgtg gcgttgctgg ggcgcaatgg cgcagggaag tcgacgttgc
      181 tgcgtctcgt cacggggctg atccaccccg cgatcggcac gatccgcgtg ggtgactggg
      241 acgctgcgtc ccggcgcccc gacgagctgg cccgccgggt aggctcgctg ttccagcacg
      301 ccgaccagca gctgttcgcc cgcacggtcg ccgacgacgt cgcgttcggg ccgcgcgtca
      361 cgggcatccc tgcgacggag acggccgagc gcgtgcgccg cgccctggag agcctcggac
      421 tgctcgaggt ggcggcggag catccgtacg atctgccggc gccctggcgc aaactcgccg
      481 cgctggccgg cgcgttggcg ctcgagccca tgctgctcgt actggacgag ccgacggccg
      541 ggctggaccg gcgatcgcgg gctcgcgtgg gccaggccct cgcggcgcga gccgacggcg
      601 gaacggcgct gctcgtggtc acccacgatc tgggcttcgc cgccgagacg cttcagcgcg
      661 ccctggtgct ggagcagggg gggttggcgc gcgacgtgac actgggccct tttcttgcgg
      721 aggcaaaggg gttggagggg ctgggactcg tggcgccgcc cctggcgcgg ctctcccgag
      781 ccttggggct gccgggcggg cccgtgcggg aaatagaggt cgcgagtgcc ctctggcagc
      841 gttgcagcgc ctagtacctt gtcctagatt cacttaccgc ccatggtcaa cccggtcgtt
      901 ttcgccacgc acctggcgcg cgctgtcgac ctgtttcggg atcccgccaa gaaggaagat
      961 cagaagcggg agttccggat tctcgtcgac ctgctgaagg aaagcggtgt cgccgcgcgc
     1021 gtcgtggacg acgtcctcca ggtaaacggt gaggcggtta cggcgccgga attccagccg
     1081 ttggtgcagc gcatggtgct gcacggggtt acggagatca tgattcccgc ggacgcgcct
     1141 gtggcgcacc tgttcgagtt gctgcgggcg ctggcggatc agccgggatc ctccttgaac
     1201 atcgtctccc ggttgcgcgc ttcgggcgcc agccgggtgt cggtcgtact ggccgcgctc
     1261 gatgagccag aggacgtgcc tcccgggcgg agcgagccgg aagcgcctga ggaaccggag
     1321 ggtccgccgg agaccgccga tatgggtacc cgcggggtgt tgcgaggcga gccggtgcgg
     1381 gatctcaaga gcaccccgat cgccggggcc gagggtgtgg agcaggtcga gcagaacctt
     1441 gcacccccat ccgtggcgct gccgagccct gatgccggca cgccgcacgg ggacgtcttc
     1501 ccgcacgaaa ctgagaaaca gccggagctt ccgtcgaccc acgtggaggg cgttgcaccg
     1561 ccgcctcagg agagcgtgcc gccactgccg gcgaacctcc cgcctcgtcc gccgccgccc
     1621 cctccgccgc cactgacaca ggcgccgacg ttccggcctt cgtccgcggc catcatgccg
     1681 cctcccttgt ccccgcagcc taccgaggcc gtcgagccgg cgccgcagtc cgagattgcg
     1741 cgcgaggcgg cgtcaccgac cttcgccctc cgtgaggccc tctccaagga cacgaccgac
     1801 ctgctcgcgt ccctggagca tgacccacag gccgccagcg tgggcgagac gctgacggtg
     1861 ctcacccgcc aggtcgagag cgccatgcgc caggggaaag tcgaggccgc gatccagatc
     1921 gtctacagcg tcgtgcgcgc cgagcggacg atagaggacg cggccgtgcg ccggcagtac
     1981 ggcatcgcgc tgcgccgcat gatcacgcgc accatgctgg acagcctcag caagctggtt
     2041 ctggtcccac acctcgagga cgcggcggcc atggtgctgc agcgcgccgg ccccgacggg
     2101 atggaggtgt tgctcgagct tctgtcgacg tcgaacacgg tcatggagcg gcgcggggtg
     2161 ttcaacgccc tcatccaaat gaaagagggg caggaccagc tcgtccacat gttgggccac
     2221 ccccagtggt tcgtggtgcg caacgtggcg gacctggtgg gcgagctggg attggaagcg
     2281 gccgtgcccg cgctcgccaa acagctcgat catcccgacg agcgggtccg caagcaggtg
     2341 gccctggcgc tcgccaagat cggcacgcgc ggggcggcgg agccgttgcg ccgcgtgctg
     2401 cgcgaccgtt cctcggaggt gcgccgtcag gccgcgctgg gcgtgggcgg ccggaaggcc
     2461 agcgcgctgg cgatgccgct ggtcgtggcg ctggaagaag agaaggaccc cgaggtggtg
     2521 cgtgagctgg tgctggcact gggtcgcatc ggcagccccg acgcggtgca ggcgctgatc
     2581 aagatcgctc agccctcggg caggctgttc ggccgcaagc cgtcggcgct gcgggtgacc
     2641 gcggtggaag cgctgcgggt ggcggggacg cccaccgcaa tcgggacgct ccagaacctc
     2701 tccaacgaca gcgacaaaca ggttcgtgcc tccgcccggg aggcgctcag cgagttgagt
     2761 ctgaagaagt cctgatccta ggtgggaatg aagcccgcgt cgggtcccag cttgcgcgcg
     2821 aacgcggcga gcaggcgggc cgcccgctcg acgtccgcca gcgccaccat ctcgttcggg
     2881 ctgtgcatgt accggttggg gaccgaaacg agaccggtgg ggatgccgcg cagcgcgttg
     2941 tggatcgcgt cggcgtcggt gccggtgtcg cgcggagccg cctgcacgct gtagggaatc
     3001 ccctcttcca cggcgcactg caccaggaaa tcgaagacca cagggttcag cgccgagccg
     3061 cggccgaaca ccggcccgcc cccgagcttg tggtcccctc cctgtttctt gtccgcgccg
     3121 ggccagtcgg tggagtgggt gacgtcgacc acgagcgcca cctgcgggtc gatgccgacc
     3181 gcgctggtgc gcgcgccccc gccggtgtac gcgatttcct cgcgcgtcgt cgccaccgcg
     3241 aacacggccg cgtggggcgg gcgctgctgc gcgagcaggc gcagcgcttc cgcgaccaca
     3301 taggcgccga tgcggttgtc gatgctgcgc gacacgatcc gcccaccggg gaactcctgg
     3361 ctggcggcgt ccagcaccgc cggatcgccc acgtgcactc gcgccagcgc cgcgtcgcgc
     3421 gaggcggcgc cgatgtcgat ccacaggtcg gtgatctgac tgaccttgtc gaaatcctcc
     3481 ttcttcatca ggtgcggcgg cttcttcccg atgacgccgc gcacggggcc cgccgggccg
     3541 accacgataa cccgctggcc caccaggacc tgggaatccc agccgccgat ccccgcgaaa
     3601 tagaggaacc cgtcctcgtc gatgtgcgtg atctgcaggc cgatctcgtc gatgtggccg
     3661 gcgaacatca ggcgcggctt gcccttcggg ttgacggtcc cgacggaatt gccgtgcacg
     3721 tcggggcgca gctcgtcggc gaaccgcttc acctcatccc gccagacgcg cgcgggggcg
     3781 gcttcgaacc ccgaggggcc gggcgaatcg aggagacgct tgagaaagtc gtgcgacgac
     3841 gcggtcatgg cagcgtggcg aagaggatga tcgggagcgc ccccaccagc cggacacgcc
     3901 ggcggacggt gtcggccgcg gtgcgggcgg cgaaggtggc ctggaatccg gagcgcacgg
     3961 ggatcacgtc cgccatcacg aatccgtccg gcgtgatcat gaaggtgaac acccccgagt
     4021 atgcggtcaa cgggttcccg cgcttgaaga tgaaattgcc catcgagtag gcgatcggct
     4081 tgccgcgata ccattccacc ggctgcagca cgtgcggatg gtgtccgagg cacaggtcgg
     4141 cgcccgcgtc caccgcgccc tccagaaacg cgcgcaatgc atcgtccggt ccatcggaga
     4201 gctcggtgcc cgcgtggatg ctcaccacca cgaagtcggc tgcgcccgat gccttgatcc
     4261 ggcggatggc gggatagagc caggcgctgt cggcgtaggc gatgtagcgc gagccgacgt
     4321 gcgagtagaa gcgccggggc gcggggttgt acgcgcgcgt caccgcgaag aaggcgaccc
     4381 gccagccctt gcgctccacg atcgccggcc ggtgcgcgtc ggccagcgtc cggccggtgc
     4441 cggtatgcgg cagcccgacg cgatcgagct gcttcaccgt ctcgaggacg ccggtgagcc
     4501 cggtgtccca ggcatggttg ttggcggtgc tgacgacgtc gaatccggcg cgcaccagcg
     4561 cgtcggcggc caccggaggg gcgctgaaca ccgggctccc cgtgtgcacg aagggctgcc
     4621 ccaccggcgc gatggggct
//
LOCUS       DBNGZW010000065         1966 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1641819, whole genome shotgun sequence.
ACCESSION   DBNGZW010000065 DBNGZW010000000
VERSION     DBNGZW010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1966
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1641819"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /gene="recA"
                     /locus_tag="AC16JC_01645"
     CDS             <1..680
                     /gene="recA"
                     /locus_tag="AC16JC_01645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013778360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR5163430.1"
                     /translation="DTGEQGLEITEILVRSGAVDIVVIDSVAALVPKAEIEGEMGDSH
                     VGLQARLMSQALRKLAGAINRSRTSVIFINQLREKIGIMFGNPETTTGGRALKFYSSV
                     RLDMRRIAPVKEREVVIGNHVRVKVVKNKVAPPFRQAEFDIMFDEGISRESLLVDIAA
                     EAGIVEKSGAWYTYGKERIGQGRENAKLYLKDHVEMAAEIEEKVKEHLGVKPVLSADE
                     EPASGEE"
     gene            684..1325
                     /locus_tag="AC16JC_01650"
     CDS             684..1325
                     /locus_tag="AC16JC_01650"
                     /inference="COORDINATES: protein motif:HMM:NF014668.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HIR5163431.1"
                     /translation="MTVTGLAPDPRRPGYCVVDVDRGRFASLPAEVVAELRLAIGREL
                     SRAELQRLQAAADEAAAARAALRALARRPHARGDLRRRLVLRQHPPDAVDRALERLAA
                     QGLLDDRQYAEHFAATRAARGRGPARLVTDLLRQGVDRRSAEAAVRAALSAEGIDPGR
                     AVRAVAERRAAQLGDLPAAVKRRRLRAYLARRGYAGRQAWAVVEQVVALYSSG"
     gene            1322..>1966
                     /locus_tag="AC16JC_01655"
     CDS             1322..>1966
                     /locus_tag="AC16JC_01655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007155537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase-related protein"
                     /protein_id="HIR5163432.1"
                     /translation="MKSAEIRNKFLEYFSASSRGHREVPSSSLVPADDPTLLFTNAGM
                     VQFKRVFQGLEHRDYARAVTCQKCVRAGGKHNDLEQVGHTARHQTFFEMLGNFSFGYY
                     FKREAIEFAWEWVTSERWLGIDPDRLYVTVHHSDDEARKLWREITKIPDSRIYGLGDK
                     DNFWQMADTGPAGPNTELNVDLRPASARRRGDVTQDEFVRLNEDGQMLEIWNLVF"
BASE COUNT          330 a          621 c          717 g          298 t
ORIGIN      
        1 ccgacacggg agagcaggga ctcgagatca ccgagattct ggtgcggtcc ggggccgtgg
       61 acatcgtcgt gatcgactcc gtggccgccc tcgtgccgaa agccgaaatc gagggcgaga
      121 tgggcgactc tcacgtgggt ctgcaggcgc ggctgatgag ccaggcgctg cgcaagctcg
      181 cgggagcgat caaccgcagt cgtacgtccg tgatcttcat caatcagctg cgcgagaaga
      241 tcggcatcat gttcggtaac ccggagacca ccaccggcgg gcgggcgctc aagttctatt
      301 cgtcggtccg gctcgatatg cggcgcattg ccccggtgaa ggagcgggag gtggtgatcg
      361 gcaatcacgt ccgcgtgaag gtggtgaaga acaaggtcgc tccgccgttc cgtcaggccg
      421 agttcgacat catgttcgac gaaggcatca gccgcgaatc gctgctcgtg gacatcgccg
      481 ccgaagccgg tatcgtggaa aagtccggcg cctggtacac ctacggcaag gagcggatcg
      541 gacagggccg ggagaatgcc aagctgtacc tcaaggacca tgtcgaaatg gcggccgaga
      601 tcgaagagaa ggtcaaggag catctcggcg tgaagccggt gctctcggcc gacgaggagc
      661 ccgccagcgg cgaggagtaa accgtgacgg tgaccggtct cgcccccgat ccgcgccgcc
      721 cgggctactg cgtggtggac gtcgatcggg ggcgattcgc gtctctgccg gcggaggtcg
      781 tcgcggagct gcggttggcc atcgggcggg agctctcccg ggcggagctc cagcgcctgc
      841 aggcggcggc cgacgaagcg gccgccgcgc gcgcggcgct ccgagccctg gcgcggcgtc
      901 cgcacgcgcg tggggatctg cggcgtcgcc tggtgctgcg acagcatccg ccggatgcgg
      961 tcgatcgagc gctggagcgt ctggcggcac agggactgct ggatgaccgg caatacgccg
     1021 agcacttcgc tgccactcgg gccgcacggg gccgggggcc ggcgcgcctg gtgaccgatc
     1081 tgctgcggca gggggtggat cggcgctcgg ccgaggccgc ggtgcgggcg gcgctgtcgg
     1141 ccgagggaat cgatcccggc cgcgcggtgc gggcggtcgc ggagcgccgg gcggcgcagc
     1201 tcggtgacct tcctgcggcg gtcaagcgac gccgcttacg ggcctatctc gcccggcgcg
     1261 gttacgccgg tcgtcaggcg tgggcggtgg tggagcaggt cgtggcgcta tattcctcag
     1321 gatgaagtct gccgaaatcc gcaacaagtt cctcgagtac ttctccgcct cctcccgggg
     1381 ccaccgggaa gtgcccagct ccagcctggt gccggccgac gacccgacgc tgctgttcac
     1441 caacgcgggg atggtgcagt tcaagcgcgt cttccagggt ctggagcatc gcgactacgc
     1501 ccgggcggtg acctgccaga agtgcgtgcg cgcaggcggc aagcacaacg acctggagca
     1561 ggtgggacac acggcccgcc accagacctt cttcgagatg ctcggcaact tctccttcgg
     1621 ctactacttc aagcgagaag cgatcgagtt cgcctgggaa tgggtcacga gcgagcgctg
     1681 gctggggatc gaccccgacc gcctgtacgt gacggtgcac cactccgacg acgaggcgcg
     1741 caagctgtgg cgggagatca cgaagatccc ggactcgcgc atctacggcc tcggcgacaa
     1801 ggacaacttc tggcagatgg cggatacggg gccggccggc cccaacaccg agctcaacgt
     1861 cgatctgcgt cctgcgtcgg cccgccgacg gggggacgtg acccaggacg agttcgtgcg
     1921 cctcaacgaa gacggacaga tgctggagat ctggaacctc gtgttc
//
LOCUS       DBNGZW010000066        12006 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_686798, whole genome shotgun sequence.
ACCESSION   DBNGZW010000066 DBNGZW010000000
VERSION     DBNGZW010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 12006)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12006)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12006
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_686798"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..935
                     /locus_tag="AC16JC_01660"
     CDS             78..935
                     /locus_tag="AC16JC_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163433.1"
                     /translation="MRVACLLLLAVFACKGKEPAPVPVITKIALRYHPPTGAVYRYVL
                     EQSSRFAPDTAAATDSSTLTSMQLVFQQHIGNRGAEGVLVTMTLDSSRVASPMLSPEA
                     ARTAAGQLRGLSVTGVVDDRLRFVRADFSSLGRLPIIVREQVELGIRAAALSFPDEAV
                     GPGDGWTIQTEVPFAQLASGSPLDVTSKITVRSITVTRGDTTVELGVETGLPDRPLEF
                     NFGGQTVSVALRGGISGSQVFSLTRGAVVTGTLGGIMHLTVTGGFFGTQGMVMRVEQR
                     ATTRLVETR"
     gene            940..2139
                     /locus_tag="AC16JC_01665"
     CDS             940..2139
                     /locus_tag="AC16JC_01665"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5163434.1"
                     /translation="MKLAHLADLHLGFRQFDRQTPRGANQREADVALAFARAVDDLLE
                     QRPDLVVFAGDIFHSVRPTNPAILFLFQQLQRIRVALPETPVILLAGTHDTPRTAETG
                     SILRLYEAVGAEVVLDTPRRLVFPKLDCAVLAVPHQALMSGERPALRPERGGPTRNVL
                     LTHFQYQALGEARGTMEYGGALLGPEDLAPAQWDYVALGHFHVAHEVFPNAWYSGALE
                     YISPNPWGQLQEEAERGRAGKGYLMVDLPGARVHFRRIALARQHIELEPIHGKDLTAR
                     QIDEKIAEAVRTAKPSIDDNVVRQLVWDVARATAHDLDHQAIRGYKARALHFQLDLRR
                     PEERYHVGVGVPARRQTLGETVRDFLSRRLLNADIPREEFVSSGVAYLERVSGGQDGT
                     GEGAAPA"
     gene            2139..4574
                     /locus_tag="AC16JC_01670"
     CDS             2139..4574
                     /locus_tag="AC16JC_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5163435.1"
                     /translation="MELKRLRLENFRQFAETEIEFGPGITGIVGPNGAGKTTLLEAIA
                     WAIYGVQAVRGDKESIRRHGAPGRSRVEVTLEFRLGAHEYVVTRNLYAAELRQDGQMV
                     ANSLKAVSEKLGRVLGMSHDEFFNTYFTGQKELAVMSSLGRTERAAFLSRVLGYERLE
                     LAQQLVREARNAIGSELKGLEAGLPDRGQLETERTQAAARLTAARKAAETARAAQKAA
                     EQGVKREEPRWKEWEARRDRVRSLESERGMGAHAVETARQEFQRLDRELADALKARTD
                     LERLATELVPLAERKAELVRLERLQEEEAARQADRRALAELARTAELRDRRIAELEGA
                     AAALGGVEAEARALAERLLAQERAVEEQRAEWARDRQDAETKRQQLRDQWRDVKEQRD
                     KIAELGPEGACPTCQRPLGAEHAAVLALLDRQLEDIAINGHFFKQRMEQLADPPETVR
                     AAEQARDAVREEARRASERAGKLRAQVEERTLALTRRAADARRASELEARLAARPTGY
                     DPARHDAVRAELARLEPLAREAAVLEARARLAEALVKEAELAEQQLSRREQQVKQLAA
                     AIAAEGFSEEKSRAAKERFDRAMGILREAELAAVEAGGALTRAEDQVAEVERRQSERA
                     GRERQIEVLKGRRRLHDELDRAYSDLRAELNAAMRPEIAELASGFLSDLTDGRYDAVD
                     LTEDYTLTALEDGVPKPVLSGGEEDLANLVLRLAISQMIAERAGQPLSLLVLDEVFGS
                     LDESRRQHVLGLLRRLGDRFPQVILITHIDQIREGLDRVIRVEYDGARGVSTARDDTA
                     TLAAGGLDAGVAA"
     gene            4555..5535
                     /locus_tag="AC16JC_01675"
     CDS             4555..5535
                     /locus_tag="AC16JC_01675"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5163436.1"
                     /translation="MRAWLPERRVDGPLPAREDDIEALNKLFAEAFTDRYRRDGLANV
                     RVPPLNPRVWTYALRDAAGGAMLWRDEDGKLVAFNVAHLCGAEGWMGPLAVRVDRQGA
                     GLGKTIVRAATEWLVDHGVTTLGLETMPRTFENIGFYAHQGFLPGHLTLTMTRELALQ
                     RRNTAAPLLASRLQPAARSESLGAAHSLADALAPGYDFTHEIELTAELGLGDTVLVAG
                     GDQLDAYALWHSASLAADRSSDELRVLKLAARHAGAFDAVLGAIEASAVHAGLRRVVI
                     RCQSRFERAFRTLVARGYQVRWTDLRMHLDGYPEPPLPEGGGVLFSNWEI"
     gene            5568..6797
                     /locus_tag="AC16JC_01680"
     CDS             5568..6797
                     /locus_tag="AC16JC_01680"
                     /inference="COORDINATES: protein motif:HMM:NF015557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsB/NhaD family transporter"
                     /protein_id="HIR5163437.1"
                     /translation="MIAGLVIFAATYVLIAVQRLPFIHLNRPAASLVGAVAMVVAGVL
                     TPQQAYAAIDLDVIVFLLGLMLIVGYLEVGKFFEWAADAVLRRAGTPPRLLAAAVVGG
                     GLLSAFFVNDTVCLVVTPVLLAALGTLRVRPTPYLLGLAMGANVGSVVSITGNPQNML
                     VGIWSGTSFGGFLARMLPVGAGGLVLTYAVLRRAFRDELARPFPEEVATAPVALDRPL
                     VTKGLAAFGVAVAGWLAGWSLPGVAIAAGALMVLAAQRDPGDAIERVDWDLLLFFASL
                     LVVMRGLEATGAVAWIDRHALALAQGASPWRDATVVSGVMVALSNLISNVPAVILWRG
                     TVPTLPDPAFVWRIVAMSATFAGNLLLIGSVANLIVAEKAEAAGIRIGFWEYARVGIP
                     VTLLTLAWGALVLVLGR"
     gene            complement(6846..7187)
                     /locus_tag="AC16JC_01685"
     CDS             complement(6846..7187)
                     /locus_tag="AC16JC_01685"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5163438.1"
                     /translation="MLTVKRWDESAGPLQLSTLRAALQQEGMTTAWWSEVPGTNVPQH
                     EHPFPESRWVLSGFLRVYVGDEIVELGPGDRLDLPPGTKHGFEVVGLSPAIYVTGSAQ
                     ATIGAVPDDRS"
     gene            complement(7232..7393)
                     /locus_tag="AC16JC_01690"
     CDS             complement(7232..7393)
                     /locus_tag="AC16JC_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163439.1"
                     /translation="MNLSLIGAVVMVAVWAALVFAVGVPSGWVHLLYVGAVLLFARRI
                     LLGAPRFLS"
     gene            complement(7748..8953)
                     /locus_tag="AC16JC_01695"
     CDS             complement(7748..8953)
                     /locus_tag="AC16JC_01695"
                     /inference="COORDINATES: protein motif:HMM:NF013832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:calcium antiporter"
                     /protein_id="HIR5163440.1"
                     /translation="MRVLKLLAPLAQAGGSALSALWTFPSILFSAFLVAWGAEAAQFL
                     MSQGLALAILAWLQTLPEFAVEAVIAWDAGRDPERAHLAIANLTGAIRLLLGLGWPMI
                     YFVHAFAGWKRGKGRLPAITLDREHAVEVVGLVPPLLYFVVILLKRSFGWIDAVVLIA
                     LYLAYLWVLLKHPPQEAERIGEAPRVAQWAYRQRGWRQKAAIFGLFAAGGALLYVTAH
                     PFLESMLAVAGTLGVSQFVLVQWVAPFLSEFPEKVSAFAWARRVTQAPMALMNLVSSN
                     INQWTVLAAMIPLVYGYSHLHHHGTWSSFHFDGPQQLEILLTLLQSGLAMLLLANMEF
                     DWLDASIIFVLWLAQFLVPHWRDEVAIAYGIWMVILVGQYAVGDKKLLAWRYFLESVR
                     EGGSGKGEG"
     gene            8993..9406
                     /locus_tag="AC16JC_01700"
     CDS             8993..9406
                     /locus_tag="AC16JC_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HIR5163441.1"
                     /translation="MPRCTVTRRVHFNAAHRLWNPRFSEEENARVFGVCNNPNFHGHN
                     YNLDVSVEGEIDPATGYVFDIHRLKTVAEECVVRHLDHKNLNLDVPWFRELNPTAENI
                     AVVCWRLLVPTVAPARLVRIRLWETERNYVDYQGE"
     gene            9409..10056
                     /gene="folE"
                     /locus_tag="AC16JC_01705"
     CDS             9409..10056
                     /gene="folE"
                     /locus_tag="AC16JC_01705"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016549492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR5163442.1"
                     /translation="MGRDQIKSASHHSFHEAHTEAGTGPEQGDFERLVHGMLVQLGED
                     PMRDGLRDTPARVAASLRWLTRGYQMSVEDVIGDAVFEEKHENMILVRDIEIYSLCEH
                     HLLPFFGRAHVAYLPNGRIVGLSKIPRVVEVFARRLQVQERLTDQVADALCRVLKPLG
                     VGVVIEAYHLCMMMRGVEKQNSKTITSALRGTFREDGMTRDEFLRLVHGGANWLP"
     gene            10083..10763
                     /locus_tag="AC16JC_01710"
     CDS             10083..10763
                     /locus_tag="AC16JC_01710"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5163443.1"
                     /translation="MALVTGASRGIGLAVADELRAAGAHTVRLARSLSDGAADRETTL
                     RCDVTRPEEVERAVHSIETDLGVPDIVVNNAGVFFVKRLDETTADMFAQSIATNLTGP
                     FLIAHAFVPAMVRRGHGHLVTIGSSSDRIAYPGNAAYAASKYGVRGLHEVIAAELAGT
                     GVHTTLVSPGAVDTEMWDPIDPDRTEGFRKRADMMRAEDVAEAVLFAVTREERVTVTE
                     IRMAPTRR"
     gene            10883..>12006
                     /locus_tag="AC16JC_01715"
     CDS             10883..>12006
                     /locus_tag="AC16JC_01715"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR5163444.1"
                     /translation="MGLIFLVGPRLGRAQQIPPLTDASGWGVHVLAIARAPDSSIWVG
                     TYGQGIFVLRRGAGALKDAGGEREWEHIRSSSDTAAHSISWDFVHAFGFGSHGEVWYG
                     TVGNGWGVSADGGRTWKNWEFRQLGPEWQYVVPNGIVTRGDTVYIATADGIKVTWDDG
                     ATWRVITDSAGATTARDSVLGRIQSQYVLAIVADTSPNMWISTLKGIGHSPDGGRTWH
                     EHFPPVPCAGAGCVNRARALSVDSGGRFWIGTEQGLYRFGPEFGGWGTADGQPCGKRA
                     SCPGGPLPVQAFTSRTLRPPGEQPRSPAPGKPRHTWFRRPIALADQPYIDQTYRFGST
                     MGGNFQAHQGVEFNNPDGTPVHAIGDGVVVYSGPAEAGANT"
BASE COUNT         1852 a         4009 c         4393 g         1752 t
ORIGIN      
        1 gaaggggcgc gcgagcgcgc cgcggtccag ccgctcaacc cgatcgccga agagccgtac
       61 tgatctcagg gagacccatg cgcgtcgcct gcctgctgct cctcgcggtg ttcgcctgca
      121 agggaaagga acccgcgccg gtccccgtga tcaccaagat cgcgctgcgc taccatccgc
      181 cgacgggcgc cgtgtaccgc tacgtgctgg agcagagctc ccgcttcgcg cccgacacgg
      241 ccgccgccac cgactcctcc accctcacgt cgatgcagct ggtgttccag cagcacatcg
      301 gaaaccgcgg cgccgagggt gtcctcgtga ccatgacgct ggattcgtcc agagtcgcca
      361 gtcccatgct gagcccggag gcggccagga cggcggcggg acagctacgc ggcctcagcg
      421 tgacgggtgt cgtggacgac cggctccggt tcgttcgcgc cgatttctcg tcgctcggcc
      481 gcctgcccat catcgtacgc gagcaggtgg agctcgggat tcgggcggcc gcgctgtcgt
      541 tccccgacga ggccgtcggc ccgggcgatg ggtggaccat ccagaccgag gtcccgttcg
      601 cccagctggc gagcggctcg ccgctcgacg tcaccagcaa gatcaccgtg cggagcatca
      661 ccgtgacgcg gggcgacacc acggtggagc tcggggtgga gacggggctc ccggaccgcc
      721 cgctggaatt caacttcgga ggacagacgg tctccgtcgc gctgcgcggc gggatcagcg
      781 gcagtcaggt gttctcgctc acgcgcggtg ccgtggtcac cggcacgctc ggcggcatca
      841 tgcacctgac cgtcacgggc gggttcttcg gcacgcaggg tatggtgatg cgggtggagc
      901 agcgcgcaac gacgcggctg gtggaaacgc gctgaaacga tgaagcttgc ccacctggcc
      961 gacctccatc tgggcttccg ccagttcgat cgccagacgc cccgcggtgc gaaccagcgc
     1021 gaggcggacg tggcgctggc gttcgcgcgg gcggtagacg acctgctgga gcagcggccc
     1081 gacttggtgg tgttcgcggg cgacatcttc cactcggtcc gccccaccaa ccccgcgatt
     1141 ctcttcctgt ttcagcagct gcagcgcatc cgcgtggcgc tgcccgagac gccggtcatc
     1201 ctccttgccg gcacgcacga cacccctcga acagccgaga ccgggagcat cctgagactg
     1261 tacgaagcgg tgggcgctga agtggtgctc gacacgccgc gacggctggt gttcccgaag
     1321 ctcgattgcg ccgtgcttgc cgtcccgcac caggccctga tgagcggcga gcggccggcg
     1381 ctccgccccg aacgcggtgg ccccacgcgc aacgtcctcc tcacccattt ccagtaccag
     1441 gccctgggcg aagcccgcgg cacgatggag tatggcgggg cgctcctcgg ccccgaggac
     1501 ctggcgcccg cgcaatggga ttacgtggcg ctgggccact ttcacgttgc ccacgaggtc
     1561 tttccgaacg cgtggtactc gggagcgttg gagtacatct cgcccaatcc gtgggggcag
     1621 ttgcaggagg aggccgaacg gggccgggcc ggcaaggggt acctgatggt ggacctgccg
     1681 ggagcgcggg tgcacttccg gcggattgcc ctggcgcggc agcacatcga gctggagccg
     1741 atccacggga aggacctcac cgccaggcag atcgacgaga agatcgcgga ggcggtacgc
     1801 acggcgaagc cgtcgatcga cgacaacgtc gtccgccagc tcgtgtggga cgtggcgcgg
     1861 gccaccgcgc acgacctcga ccatcaggcg atccgcggct acaaggcgcg cgcgctgcac
     1921 ttccagctcg acctgcggcg ccccgaggag cgctatcacg tcggcgtcgg cgtgcccgcg
     1981 cgccggcaga ccctgggcga gacggtgcgc gattttctct cgcgccgcct cctcaatgcc
     2041 gatatcccgc gcgaggagtt cgtgtcgagc ggcgtggcgt acctggagcg ggtgagcggc
     2101 gggcaggacg gcacggggga gggggcggcg cccgcctgat ggagctcaag cgcctgcggc
     2161 tcgagaactt ccggcagttc gccgagaccg agatcgagtt cggtcccggc atcaccggga
     2221 tcgtcggtcc caacggggcc gggaagacga cgctgctcga agcgatcgcc tgggcgatct
     2281 acggcgtgca ggcggtgcgg ggcgacaagg agtcgatccg gcggcacggg gcgccgggcc
     2341 ggtcgcgcgt ggaagtgacg ctcgagttcc ggctcggcgc ccacgagtac gtggtcaccc
     2401 gcaacctcta tgcggccgag ctgcggcagg acggccagat ggtggcgaac tcgctcaaag
     2461 cggtgtcgga gaagctgggg cgggtgctcg gcatgtcgca cgacgaattc ttcaacacgt
     2521 acttcacggg gcagaaggag ctggccgtga tgtcgtcgct ggggcgcacc gagcgcgccg
     2581 ctttcctgtc gcgcgtcctg ggctacgagc ggttggagct ggcgcagcag ctggtgcgcg
     2641 aggcgcgcaa tgccatcgga agcgagctga aggggctgga ggcgggcctg ccggaccgcg
     2701 gacagctcga aaccgaacgg acccaggcgg cggcccggct gacggccgcc cgcaaggccg
     2761 cggagacggc gcgtgccgcg cagaaggcgg ccgagcaggg agtgaagcgc gaggagcccc
     2821 gctggaagga atgggaggcg cgccgcgacc gggtccgctc gctcgagagc gagcggggca
     2881 tgggcgcgca cgcggtggag acggcccggc aggaatttca gcggctcgac cgggagctgg
     2941 ccgacgcgct gaaggcgcgg accgacctgg agcgcctggc caccgagctg gtgccgttgg
     3001 cggaacgcaa ggccgagctg gtccgcctgg agcggttgca ggaggaagag gcggcgcggc
     3061 aggccgatcg ccgggcgctg gccgagctgg cccggaccgc ggagctgcgg gaccggcgta
     3121 tcgcagagct cgagggtgcc gcggcggcgc tcgggggcgt ggaggccgag gcgcgcgcgc
     3181 tcgccgaacg gctcctggcg caggagcgtg cggtggagga gcagcgggcc gaatgggcgc
     3241 gcgaccgtca ggacgccgag acgaagcggc agcagctgcg cgatcaatgg cgcgatgtga
     3301 aggagcagcg cgacaagatc gccgagctgg ggcccgaggg ggcgtgcccg acgtgccagc
     3361 ggcccctcgg agccgaacat gcggccgtcc tggcgctgct ggatcgccag ctcgaggaca
     3421 tcgccatcaa cgggcatttc ttcaagcagc ggatggagca gctggcggac cccccggaga
     3481 cggtccgtgc cgcggagcag gcgcgcgatg cggtacgcga ggaggcccgg cgcgcgagcg
     3541 agcgggcagg gaagctgcgc gcccaggtcg aggagcggac gctggcgctg acacgccggg
     3601 cggcggatgc ccggcgcgcg tccgagctcg aggcgcggct ggcggcccgg cccacgggct
     3661 acgatccggc gcggcacgac gcggttcggg cggagctggc gcggctggag ccgctggcgc
     3721 gcgaggcggc ggtgctcgag gcccgcgcgc ggctcgccga agcgctggtg aaagaagccg
     3781 agctggcgga gcagcagctg tcgcgacgcg agcagcaggt gaagcagctc gcggcggcca
     3841 tcgccgcgga ggggttctcc gaggagaagt cccgggcggc caaggaacgc ttcgaccgtg
     3901 cgatggggat cctgcgggaa gcggagctgg cggcggtgga ggccgggggc gctctgacgc
     3961 gcgcggagga ccaggtggcc gaggtggagc ggcgccagag tgagcgggcg gggcgcgagc
     4021 ggcagatcga ggtgctcaag gggcggcgtc gcctgcacga cgagctcgat cgcgcctact
     4081 cggacctgcg ggcggagctg aacgccgcga tgcgtcccga gatcgccgag ctggcctcgg
     4141 ggttcctctc cgacctgacc gacgggcgct acgacgcggt ggacctgacc gaggactaca
     4201 cgctgaccgc cctcgaggac ggcgtgccga agccggtgct ctcggggggc gaggaggacc
     4261 tggccaatct cgtgctgcgg ctggcgatct cgcagatgat cgccgagcgg gccgggcagc
     4321 cgctgtcgct gctggtgctg gacgaagtgt tcggctcgct cgacgaatcg cgccgccagc
     4381 acgtgctcgg cctgttgcgc cgactcggcg accgcttccc ccaggtcatt ctcatcaccc
     4441 acatcgacca gatccgggag ggactggacc gcgtcatccg cgtggagtac gatggcgcgc
     4501 ggggcgtgag taccgcacgg gacgatacgg cgaccctggc tgcgggaggg ctggatgcgg
     4561 gcgtggctgc ctgaacggcg cgtggacggc ccgctgccgg cgcgcgagga cgacatcgag
     4621 gcgctgaaca agctgttcgc cgaggcgttc accgaccgct accggcgcga cgggttggcg
     4681 aacgtgcgcg tgccgccgct caacccccgg gtgtggacct acgcgctgcg cgatgcggcg
     4741 ggcggggcga tgctgtggcg tgacgaggac gggaagctcg tcgcgttcaa cgtggcgcat
     4801 ctgtgcggcg cggaagggtg gatggggccg ctggcggtgc gcgtggaccg ccagggcgcg
     4861 gggctcggca agacgatcgt gcgcgccgcg accgagtggc tggtcgacca cggcgtcacg
     4921 acgctcggcc tcgagaccat gccgcgcacg ttcgagaaca tcggcttcta cgcccaccag
     4981 gggttcctgc ccggccacct cacgctcacc atgacgcgcg agctcgcgct gcagcggcgc
     5041 aacaccgccg cgccgctcct ggcctcccgc ctgcaacccg ccgccaggag cgagtcgctg
     5101 ggcgcggcgc actcgctcgc cgacgcgctc gccccgggat acgacttcac gcacgagatc
     5161 gagctgacgg cggagctggg cctgggcgac acggtgctgg tcgcgggggg cgaccagctc
     5221 gacgcctatg cgttgtggca ttcggcctcg ctcgccgccg atcgatcttc cgacgagctg
     5281 cgcgtgctca agctcgcggc gcgccacgcg ggcgccttcg acgccgtgct cggcgcgatc
     5341 gaagccagcg ccgtgcacgc cgggctgcgc cgcgtcgtga tccgttgcca gagccgcttc
     5401 gaacgcgcgt tccgcaccct cgtcgcccgc ggctaccagg tccgctggac cgacctgcgc
     5461 atgcacctcg acggctatcc ggagccgccg ctgcccgagg gtggcggcgt gctgttctcg
     5521 aactgggaga tatgacctct ccccctgggg gactcggggg aggaggaatg atcgccggcc
     5581 tcgtcatttt cgccgcgact tacgtcctga tcgcggtcca gcgccttccg tttatccatc
     5641 tcaaccgccc ggccgcgagt ctcgtcgggg cggtggccat ggtcgtggcc ggcgtgctca
     5701 cgccgcagca ggcctacgcc gcgatcgacc tcgacgtcat cgtcttcctg ctcggcctga
     5761 tgctgatcgt cgggtacctc gaggtgggga agttcttcga atgggcggcg gacgcggtgc
     5821 tgcgccgggc cgggacgccg ccgcgccttc tggcggccgc cgttgtgggt gggggactgc
     5881 tctcggcgtt cttcgtgaac gacaccgtct gcctggtggt cacgccggtg ctgctcgccg
     5941 cgctcggcac gctgcgcgtg cgacccacgc cgtacctcct cgggctcgcg atgggcgcca
     6001 atgtcggatc ggtcgtctcg atcacgggga atccccagaa catgctggtc ggcatctgga
     6061 gcgggaccag cttcgggggg tttctggcgc ggatgctgcc ggtgggggca ggtggcctcg
     6121 tgctcaccta tgccgtgctg cgccgggcgt tccgcgacga gctcgcccgg ccgttccccg
     6181 aggaggtcgc gacggcgccc gtggcgctcg atcgaccgct cgtgacgaag gggctcgccg
     6241 cgttcggcgt cgcggtcgcc ggctggctcg ccggctggtc gctcccgggc gtggccatcg
     6301 ccgccggggc cttgatggtg ctcgcggcgc agcgcgatcc gggcgacgcg atcgagcgcg
     6361 tggactggga cctgctgctg ttcttcgcgt cgctgttggt cgtgatgcgg ggcctcgagg
     6421 ccaccggcgc ggtcgcctgg atcgaccgtc acgcgctggc gctggcacag ggcgcgtccc
     6481 cgtggcggga cgcgacggtg gtgagtgggg tgatggtcgc gctgtccaac ctgatctcca
     6541 acgtgcccgc ggtcattctc tggcgcggca cggtccccac gctgccggac ccggcgttcg
     6601 tgtggcgcat cgtcgccatg agcgcgacgt tcgccggcaa cctgctgctg atcggctcgg
     6661 tggcgaatct gatcgtggcg gagaaggccg aggccgccgg gattcgcatc gggttctggg
     6721 agtacgcccg ggtggggatc ccggtgacgc tgctgaccct ggcctgggga gccctcgtcc
     6781 tggtgctcgg ccgttagtcg cccctgaccc tgggcggcgt agtcgaatcg agccgctgca
     6841 ccaggctacg accggtcgtc ggggacggcc ccaattgtgg cttgcgcgga tcccgtcacg
     6901 tagatcgcag gcgacaatcc caccacctcg aacccgtgct tggtgccggg cggcagatcg
     6961 agccggtcgc cgggacccag ctccacgatc tcgtcgccga cgtatacgcg aagaaatccc
     7021 gacaacaccc agcgcgactc tggaaacgga tgctcgtgct gggggacgtt ggtcccgggg
     7081 acctccgacc accaggcggt cgtcatcccc tcctgctgca gggcggcccg cagcgtggaa
     7141 agctgcagcg gcccggcgct ttcgtcccag cgcttcaccg tgagcatgag gctggaacgc
     7201 tatgccgcgc tccgcccgga cgccagtttc gtcacgacag gaacctcggc gccccgagga
     7261 ggatccgccg ggcgaagagc aggacggccc ccacgtagag caggtgcacc cagccactcg
     7321 gcacccccac ggcgaagacg agtgcggccc aaacggccac catcacgaca gcgccgatca
     7381 gcgacaggtt catcggtcac acctcctgtg cggtgatagg agtcacccgg ccttcgacct
     7441 cacgggtttc tcatccactc ttccttgacg ttttcgcgcg cccttgtatc gtaggggcgg
     7501 ggacggggag ccacgttatt gagccaacag ctcgatcgtg gacccatata ctgagaccga
     7561 tgtcatgccc ccgcgagggg gaagacagac gccccggtga gggaccagcc attactttcg
     7621 ggcttcaata atcctctccg ttcctgctgt cctcgatcgg ccgggccgcg caagcggtgc
     7681 cggccgttcg tttcggcagc tggggaaggg ggaagcggga agagggaagg ggtcgcctgc
     7741 ggcgcatcta cccttccccc ttcccgcttc ccccttcccg tacactctcc agaaagtatc
     7801 gccacgccag cagcttcttg tccccgacgg cgtactgccc gacgaggatc accatccaga
     7861 tcccataggc gatcgccact tcgtcgcgcc agtgcggcac gaggaactgc gccagccata
     7921 gtacgaaaat gatactcgcg tcgagccagt cgaactccat gttggcgagc agcagcatgg
     7981 cgagcccgct ctgcagcagc gtgagcagga tctcgagctg ctgggggccg tcgaaatgga
     8041 aactggacca ggtcccgtgg tggtgcaggt gcgagtagcc gtacaccagc gggatcatcg
     8101 cggcgagcac ggtccactgg ttgatgttgc tcgagaccag gttcatcagg gccatggggg
     8161 cctgcgtgac gcggcgcgcc cacgcaaacg ccgacacctt ttcggggaac tccgaaagga
     8221 acggcgccac ccactgcacc agcacgaact ggctcacgcc gagcgtcccc gccaccgcga
     8281 gcatggactc gaggaagggg tgggcggtga cgtacagcag cgcgcccccc gcggcgaaca
     8341 acccgaagat ggccgccttc tggcgccagc cgcgctggcg gtacgcccac tgtgccacgc
     8401 gcggcgcctc cccgatgcgc tcggcctcct gcggcggatg cttcagcagg acccacaggt
     8461 aggcgaggta cagggcgatc agcacgacgg catcgatcca gccgaacgag cgcttgagca
     8521 gaatgacgac gaaatacagg aggggcggga ccagccccac cacttccacg gcgtgctcgc
     8581 gatccagcgt gatcgcgggc agccggccct tgccgcgttt ccagccggcg aaggcgtgca
     8641 cgaagtagat catcggccag ccgagtccca gcagcaggcg gatggcgccg gtgaggttgg
     8701 cgatcgcgag atgcgcgcgc tcggggtccc gtccggcatc ccaggcgatc accgcctcga
     8761 ccgcgaactc ggggagggtc tgcagccagg cgaggatcgc cagcgccaga ccctgggaca
     8821 tcaggaactg cgccgcttcg gcgccccacg ccacgaggaa cgccgagaac aggatggagg
     8881 gaaaggtcca cagcgccgac agcgccgagc ctcccgcctg ggcgagcggg gcgagtagct
     8941 ttaggacgcg cacgtgcgcc gaagataccc agagccggag tcgctacaag ccatgccgcg
     9001 ctgcaccgtg acccgtcgcg tgcatttcaa tgccgcgcac cgtctgtgga acccgcggtt
     9061 ctccgaggag gagaacgcgc gcgtcttcgg cgtgtgcaat aatccgaatt tccacggaca
     9121 caactacaac ctggacgtct cggtcgaggg tgagatcgac cccgcgaccg ggtacgtgtt
     9181 cgatatccac cggctgaaga ccgtcgctga agaatgcgtg gtccgccatt tggaccacaa
     9241 gaacctgaac ctcgacgtcc cctggttccg ggagctcaac ccgaccgccg agaacatcgc
     9301 cgtcgtgtgc tggcggctgc tggtgccaac cgtcgcgccc gcacggctgg tgcgcatccg
     9361 gctgtgggag accgagcgta actacgtcga ctaccagggg gaatgacgat gggacgcgat
     9421 cagatcaaat cggccagcca ccacagcttt cacgaggcgc ataccgaagc cggtacgggc
     9481 ccggagcagg gagatttcga gcggctggtg cacggtatgc tcgtccagct gggggaagac
     9541 cccatgcgtg acggattgcg cgatacgccg gcccgcgtgg ccgcctcgct gcgctggctc
     9601 acccgcggct atcagatgtc ggtcgaagac gtgatcggcg acgcggtgtt cgaggagaaa
     9661 cacgaaaaca tgattctggt gcgcgacatc gagatctact cgctgtgcga gcaccacctg
     9721 ctgccgttct tcggccgggc gcacgtggcc tacctcccca acggccgcat cgtggggctg
     9781 tccaagatcc cgcgcgtggt ggaggtgttc gcgcgccgcc tccaggtgca ggagcggctc
     9841 accgaccagg tggccgatgc gctgtgccgg gtgctgaagc cgctgggcgt gggcgtggtg
     9901 atcgaggcgt accacctctg catgatgatg cggggcgtgg agaagcagaa ctcgaaaacg
     9961 atcacctcgg cgctgcgcgg cacgttccgc gaggacggca tgacgcgcga cgagttcctg
    10021 cgcctggtgc acggcggggc caactggctg ccctgaccgc cgccgggccg ctgcggggca
    10081 aggtggccct cgtcacgggc gcctcgcgcg gcatcgggct cgccgtggcg gacgagctgc
    10141 gggccgccgg cgcgcacacg gtgcggctgg cgcgctccct ttccgatggc gcggccgacc
    10201 gcgaaaccac gctgcgctgc gacgtgacgc ggcccgagga agtggagcgg gcggtgcaca
    10261 gcatcgagac ggatctcggc gtcccggaca tcgtcgtgaa caatgccggg gtcttcttcg
    10321 tgaaacggct ggacgagacg acggcggaca tgttcgcgca gtcgatcgcc accaacctga
    10381 ccgggccgtt tctcatcgcg cacgcgttcg tcccggcgat ggtgcggcgc ggtcacgggc
    10441 atctggtgac catcgggtcc tcgtcggacc gcatcgccta tcccgggaac gccgcctacg
    10501 cggccagcaa gtatggcgtg cggggcctgc acgaggtgat cgccgccgag ctcgcgggca
    10561 cgggggtgca caccacgctc gtctccccgg gcgccgtgga tacggagatg tgggacccga
    10621 tcgatcccga tcggacggag ggcttccgca agcgcgccga catgatgcgc gcggaggacg
    10681 tggcggaagc ggtgctgttc gcggtgacga gggaggagcg ggtgacggtg acggagattc
    10741 gcatggcgcc gacgcggagg tgaaaccaat gcggattgcg gaatgccgaa tgcggaatgg
    10801 tggtggtgag cccgcgccga ctcgtgggga tcgatgtcgg cccggccgtt ccgcattccg
    10861 agttccgcat tccgcattcg cggtggggct gatcttcctg gtcgggccga ggctcggccg
    10921 ggcccagcag atcccgccgc tgaccgatgc atcggggtgg ggcgtgcatg tgttggccat
    10981 cgcccgggcg ccagactcat ccatttgggt ggggacgtac gggcagggaa tctttgtgct
    11041 gcggagggga gcgggggccc taaaggacgc cggtggggag cgggagtggg agcacatccg
    11101 gtcttcctcg gacactgccg cccattcgat ctcgtgggat ttcgtgcacg cgttcgggtt
    11161 cgggtcgcac ggcgaagtct ggtacgggac cgtcggcaac ggctggggcg tctcggccga
    11221 cggcggacgc acctggaaga actgggaatt ccgccagctg ggacccgaat ggcaatacgt
    11281 ggtgcccaac gggatcgtca cccgcggcga caccgtctac atcgccaccg cggacgggat
    11341 caaggtcacc tgggacgacg gcgcgacgtg gcgggtaatc acggactcgg cgggcgccac
    11401 gacggcgcgc gattcggtcc tcggccgcat tcagagtcag tacgtgctcg ccatcgtcgc
    11461 ggacacgagt ccgaacatgt ggatcagcac gctcaagggg atcgggcatt ccccggacgg
    11521 ggggcgtacg tggcacgagc acttcccgcc ggtgccgtgc gcgggcgcgg gatgcgtcaa
    11581 tcgcgctcgc gcgctgtcag tggactcagg cggcaggttc tggatcggga ccgagcaggg
    11641 gttgtaccga ttcgggccgg aattcggcgg gtgggggaca gccgacggac aaccgtgcgg
    11701 gaagcgcgcg tcgtgtccgg gcggaccgct gcccgtccag gccttcacct cccgcaccct
    11761 ccgcccaccg ggcgagcagc ctcggtcgcc ggcgccgggg aagccgcggc acacctggtt
    11821 tcgccgcccc atcgccctcg ccgaccagcc ctacatcgac cagacctacc gcttcggctc
    11881 gacgatgggc ggcaacttcc aggcacatca gggcgtcgag ttcaacaacc ccgacggcac
    11941 gcccgtgcac gccatcggcg acggcgtcgt cgtctactcg gggcctgccg aagcgggagc
    12001 gaacac
//
LOCUS       DBNGZW010000067         3337 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_891729, whole genome shotgun sequence.
ACCESSION   DBNGZW010000067 DBNGZW010000000
VERSION     DBNGZW010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_891729"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1346)
                     /locus_tag="AC16JC_01720"
     CDS             complement(<1..1346)
                     /locus_tag="AC16JC_01720"
                     /inference="COORDINATES: protein motif:HMM:NF019872.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HIR5163445.1"
                     /translation="MPLSHTLGFPPLGTARELKRATEGYWSGKVSRSDLLRTGAELRA
                     RHWRLQRAAGIDLPPSNDFSFYDRVLDTCALVGAVPPRYGWSGGDVDLDTYFAMARGV
                     QQKGRDVTAMEMTKWFDTNYHYIVPELARDQSFHLGSSKPVDEFAEALALGVRTTPVL
                     IGPLTFLLLAKAKGAAFDRLDLLDGMLPVYAEILQQLGKAGAEWVQVDEPCLGLDRTA
                     AELERYRAAYGFLAERSGSAKILLATYFAGLDQNAATALALPTHGLHVDLARAPGQLD
                     TLLERWPAGRVLSAGVVDGRNIWRTDLEAALSLLERATAKVGPDYLWVAPSCSLLHVP
                     IDLELETALDPEIRPWLAFATQKLGEVAVLTRALREGRGAVEEALADSRRAAASRRVS
                     PLTLNAVVRQRAASVQESDLRRPTPYADRRHRQLELPRYPTTTIGSFPQTADVRAAR"
     gene            complement(1462..2169)
                     /locus_tag="AC16JC_01725"
     CDS             complement(1462..2169)
                     /locus_tag="AC16JC_01725"
                     /inference="COORDINATES: protein motif:HMM:NF025679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HIR5163446.1"
                     /translation="MSHVFVRLTDAPAGITSAHAWISTVYLIGGDGTSRFTISTGPST
                     DYDLLALQGGVTTLLGDNTIPAGDYEQLRLVVDSAFVLLDGETDPRTLKVPSGMQTGI
                     KVEWNGPVHIQGGRTDLTVDFDVSRSFIITGPTPPRQVLFKPVLHGVVTDLSGSISGT
                     SSPATALGRVFAIVGTDTVATADADPTTGAYTIMFLPAATYTVLDSSTVSGHKPPTQS
                     VVVAASQNVTGVDFTIP"
     gene            complement(2336..2998)
                     /locus_tag="AC16JC_01730"
     CDS             complement(2336..2998)
                     /locus_tag="AC16JC_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163447.1"
                     /translation="MNRALTLLSLAALMVPGLAAQNDAAARLAGRVPPALVGAVTALV
                     DSARARGLPGTPLVDKALEGAVKGVPADRILPAVHTVLDQLDASAGALRGAGAAAPGD
                     QAIEAGAFAIAAGLTVSDVAAIAGAADKGHPTAVALQVAGALAALGVPRPETVGLVRA
                     EIRSGRPLGDLVSLPGQVQAAMAGGALPAAAAQGLERAAATHQAPRPSGQAKGPGNPH
                     KP"
     gene            complement(2995..>3337)
                     /locus_tag="AC16JC_01735"
     CDS             complement(2995..>3337)
                     /locus_tag="AC16JC_01735"
                     /inference="COORDINATES: protein motif:HMM:NF027875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HIR5163448.1"
                     /translation="PASVATPEEASLRETAAGPESVYVRFVLSAPHARHVALAGTFNR
                     WDPNATPLVRVGEEGTWTVTVPLTAGQHQYAFVVDGQRWVPDPAAPKVDDGFGRKNSV
                     VSVSATQGRVL"
BASE COUNT          532 a         1151 c         1157 g          497 t
ORIGIN      
        1 cgcgccgcgc gcacgtcggc agtttgggga aacgacccga tcgtggtcgt gggataacgc
       61 ggcagctcga gctgccggtg gcggcgatcg gcgtacggcg tcggccggcg cagatcactc
      121 tcctgcacgg aggccgcacg ttgccgcacg acggcgttca gggtgagcgg ggaaactcgg
      181 cgcgaagccg cggcacgccg gctgtccgcc agcgcctcct ccacggcacc acgtccctcg
      241 cggagcgcgc gggtgagcac cgctacctct cccagcttct gtgtagcgaa ggcgagccaa
      301 ggcctgatct ccggatcgag agccgtttcc agctccagat cgatcggcac gtgcagcagc
      361 gagcaggacg gggcgaccca caggtagtcg gggcccacct tcgcggtggc ccgctcgagt
      421 aacgatagtg ccgcctcgag atcggtgcgc cagatgttcc ggccatccac gacgcccgcg
      481 gacagcacac gccccgccgg ccaccgctcg agcaatgtgt ccaattgccc gggagcccgc
      541 gccaggtcga cgtgcaggcc gtgcgtcggc agggcgagtg cggtggcggc gttctggtcc
      601 agtccggcga agtaggtcgc caggaggatc ttagcggatc ctgagcgctc ggccagaaat
      661 ccgtacgccg cacggtagcg ctcgagctcg gccgccgtgc ggtcgagccc gaggcacggc
      721 tcgtccacct gcacccactc cgcgcccgcc ttccccagct gctgcagaat ctcggcatac
      781 accggcagca ttccgtcgag caggtcgagg cgatcgaacg cggcgccttt cgccttcgcg
      841 agcagcagga aggtcagcgg gccgatcagg accggcgtag tgcggacgcc cagggcgagt
      901 gcctccgcga actcgtcgac cggcttcgag gacccgaggt ggaacgactg atcgcgcgcc
      961 agctcgggga cgatgtagtg gtagttggtg tcgaaccact tcgtcatctc catcgccgtc
     1021 acgtcccggc ctttctgctg cactccccgc gccatcgcga agtaggtgtc gagatcgacg
     1081 tcgcctccgc tccagccgta tcgcggcggg acggccccga cgagtgcgca ggtgtcgagc
     1141 acccggtcgt agaaggagaa atcgttggag ggcgggaggt cgatccccgc cgcgcgctgc
     1201 agccgccagt gacgggcgcg cagctcggcg ccggtgcgca gcaggtcgga gcgggagact
     1261 ttgccgctcc agtagccttc ggtggcgcgc ttcagctcgc gcgccgtgcc gagcggcgga
     1321 aacccgagtg tatgtgagag gggcatggcg cggggaatct agcgaggaaa ccgccatctc
     1381 tccacaaacg acagggggcg gacgcttcgt gccgtccgcc ccctgtgcta cccttcgtcc
     1441 cggaatctca gccttcgagc atcacggaat cgtgaaatcc acccccgtga cgttctggct
     1501 tgcggcgacc accacggatt gcgtcggggg cttgtggcca cttacggtag aactgtcgag
     1561 aaccgtgtac gtggccgccg gcaggaacat gatcgtgtac gccccggtcg tcggatccgc
     1621 atccgctgtc gcgaccgtat ccgtcccgac gattgcgaac accctcccga gcgccgtcgc
     1681 agggctcgat gtgcccgaaa tcgatcccga gagatcggtc acgacgccgt ggagcacagg
     1741 cttgaatagc acctgacgcg gcggggtcgg cccggtgatg atgaagctcc gagaaacatc
     1801 gaagtcgacc gtgagatcgg tccgcccgcc ctggatgtgg acggggccat tccattcgac
     1861 cttgatcccg gtctgcatgc cgctcgggac cttgagggtt ctggggtccg tctcaccgtc
     1921 cagcagcaca aacgccgaat cgacgaccag gcgcagctgc tcgtagtctc cggctggaat
     1981 cgtgttatcg ccgagcagcg tagtgacgcc accctgcagg gccagcaggt cgtagtcggt
     2041 cgacggtccg gtgctgatcg tgaaacggga ggttccgtcc cccccgatga gatagactgt
     2101 cgaaatccac gcgtgcgcgg aagtgatacc ggcaggggcg tcagtcagcc gaacgaagac
     2161 atggctcatc tgaggtccgg cgccgccctc gcaggccaca aggccggctg ccaaggtcag
     2221 cgcgtagtac ttcgtgattc gcatggtttg ccgggctccc acgtaaaggg ttggtggctc
     2281 tcgttatgaa gagcgcccac ccaggggcca ggtaacgccc tgtgatggcg aaatcttagg
     2341 gcttgtgcgg gttgcccggc cctttggcct gaccactggg gcgcggagcc tgatgcgtcg
     2401 ccgcagcacg ctcgaggccc tgcgccgctg ccgccggcag ggcccctccg gccatggcgg
     2461 cctggacctg acccggaaga ctcaccaggt cgcccagcgg gcggccggac cgaatctccg
     2521 cccgcaccag accgacggtc tcggggcgag ggacgccgag cgcggccaac gccccggcga
     2581 cctgcagcgc gacggcggtt ggatgaccct tgtccgccgc gcccgcgatc gcagccacgt
     2641 ccgacacggt caggccggcg gcgatggcga acgcgccggc ctcgattgcc tggtcgccgg
     2701 gtgcggcggc gccggcccca cggagggcgc cggcggaagc gtcgagctgg tcgagcacgg
     2761 tgtgcaccgc cggcaggatg cgatccgccg gcacgccctt cactgctccc tcgagggcct
     2821 tgtccaccag gggagtaccg ggcagcccgc gtgcccgcgc cgaatccacc agagcggtga
     2881 ccgcaccgac cagggcgggc ggaacccgcc cggcgagccg cgcggccgca tcgttctgcg
     2941 cggccagtcc cgggaccatg agggccgcca ggctcagcaa cgtgagcgcg cgattcatag
     3001 cacccgtccc tgcgtggcgc tgacgctgac cacgctgttc ttgcggccga acccgtcgtc
     3061 cacctttggc gcggcgggat ccgggaccca gcgctggcca tccacgacaa acgcgtactg
     3121 atgctgtccg gcggtgaggg gaacggtgac ggtccaggtc ccctcctcgc cgacgcggac
     3181 cagcggcgtc gcgttcgggt cccaacggtt gaacgtgccg gcgagggcca cgtgccgggc
     3241 atgaggcgcg gacagcacga accgcacgta gacgctctcc ggaccggcgg ccgtctcgcg
     3301 gagggaggcc tcctccggtg ttgcgacgga cgccggc
//
LOCUS       DBNGZW010000068         8230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_756613, whole genome shotgun sequence.
ACCESSION   DBNGZW010000068 DBNGZW010000000
VERSION     DBNGZW010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8230
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_756613"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(280..1257)
                     /locus_tag="AC16JC_01740"
     CDS             complement(280..1257)
                     /locus_tag="AC16JC_01740"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR5163449.1"
                     /translation="MLLHLARSQFQAIRRAPETRDSVLVARQALGRRLFFDPRWSLDG
                     KVSCATCHPTSHYGADTGATSVGVLGRRTPRNVPTVLNATFQFAQHWRGDRRDLEDQA
                     LKALTGPQSGGHASLDDAERRLRALRDYATQFAAAFPRDTPTVSGHHFADAVAAYERT
                     LLTPSRFDAYLDGDATALSAAELAGLRRFLDIGCSGCHNGVGVGGGSFQRFGVVENYW
                     LATKSTHVDSGRFDVTHVDADRYVFKVPSLRNAAETGPYFHDGSVATLAEAIRVMARV
                     QLHRRLEDADVRTIAAFIRSLTGGLPSSFAPPQANRTSSSGKPAATKPD"
     gene            complement(1465..3018)
                     /gene="pdxR"
                     /locus_tag="AC16JC_01745"
     CDS             complement(1465..3018)
                     /gene="pdxR"
                     /locus_tag="AC16JC_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015248033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070279 - vitamin B6 binding [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:2000142 - regulation of DNA-templated
                     transcription initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MocR-like pyridoxine biosynthesis transcription
                     factor PdxR"
                     /protein_id="HIR5163450.1"
                     /translation="MSPILQVDMDPVLATPLFRQVYDGVRAAILSGRLAAGARLPSSR
                     ALAADLRVARNTVLQAFEQLRSEGYLATRRGGGTRVRSTMPDAALEPPTPTHARAQRR
                     TEGSTRSAQPLPRLSKRAALVMRSGSGFVRTSGSTSAPFELGISAVDAFPTRLWARFA
                     ARRWRQQVVGLGHADPAGEYPLRNAIADYLGAARRARCTADQVIVVHGAQQALHLIGQ
                     VLLDPDELVWIENPGYVGAWMAFQTAGARLIAVPVDQEGLDVAAGKRAARGARLAYVT
                     PSHQFPLGVLMSAPRRLELSAWARQVSAWIVEDDYDSEFRYGGRQLPCLQGLDTDTSQ
                     DGTSSRVIYVGTFSKTLAPSLRLGFLVVPDALIDAFRAMRTATDRHSPTTDQGVLADF
                     IGEGHYSRHIRQVRTLCAERQAALLAAAEAKLGGLLAVEPDAAGLHLVGRLPVGVDDR
                     RATALAAAEGVLVWPLSRFTIGTRRPSTPGALLLGYAAHSAAQIHQAAERLARALDRA
                     LKESAGAIS"
     gene            3105..4637
                     /locus_tag="AC16JC_01750"
     CDS             3105..4637
                     /locus_tag="AC16JC_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5163451.1"
                     /translation="MQPLHPGVAVSAGRREWASLAVMALPCLLYAMDGTVLNLAVPSL
                     SAALTPSSTELLWIVDIYGFFTASALVTMGTLGDRIGRRRLLLIGAAAFGIASVLAAF
                     ATSARMLIAARAILGLAGATLAPSTLSLVRNIFLDPQERSLAIGLWTASFSVGAAIGP
                     AIGGLLLQRFWWGSVFLVSVPVMGALLVLGPWLLPEYRDSRAGRMDFVSAALSLIGVL
                     ALIYGLKRAAVDGVGPLPGLWALAGMAACVLFGWRQRRVTEPLIDLGLFRAPAFNAAL
                     ATNALSLFTAFGSFLFTAQYLQSVLGLPPLAAGLWTVPSSGGSIVGSLLAPLIGRRVR
                     SRTVILGGLGLAVIGFGVLTQVQDAAGLPLVVTGSITLAVGVAAVVTVSTDIIVGAAP
                     PERAGAASAISETGAELGGALGIAILGSIGTAVYRRVVASALPFGLSETAADTVRQTV
                     GGALAEARRLPESLGAAVADVARAAFIDAFRTTAAVSAVIALSIAALAAALLRRERAD
                     LP"
     gene            4634..5077
                     /locus_tag="AC16JC_01755"
     CDS             4634..5077
                     /locus_tag="AC16JC_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007955998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HIR5163452.1"
                     /translation="MMLRWVVATAHLLALPLGLGAVWARSRALASVESPTGLGSAFAA
                     DTVWGLAAALWITTGLARAFGGLEKGTDYYLHDRVFYVKMGLFLVVLLLEMRPMITLL
                     RWRSTVRRGDSPDTTAAPALARISRIQAGLVIIMVFAATALARGW"
     gene            complement(5165..7585)
                     /locus_tag="AC16JC_01760"
     CDS             complement(5165..7585)
                     /locus_tag="AC16JC_01760"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5163453.1"
                     /translation="MNSVLLDFRYAVRGLRKTPGLALLAIICMGLGIASVTVMFSTAE
                     TFSFRPLPQVRDAGRVMHVWEAPADAPRGSDGMAPATYRAARALPEFSALAAQRGWSA
                     NISGIDVPERVGAALVSANFLRTIGRAPALGRDFTEADDEWGAGHVVILSHGLWQRRF
                     GGDTALVGRTVRINGEEYTVVGILPQDFTFPAGTELLAPLALSPESWAVRRGQGVFVL
                     GRLARGVSPSGAEAAVAALGARLAADFPATNAGWMMRAEPAEQYYGQGPRPFMMVLLA
                     AGAFVLLIACANVANLLFARATGRRRELAVRIAMGAPYARLVRQQLAESLLIGAAGGV
                     LGIFGTLGGLRAMATSVPVEVRAFIPGFAELRLDPRALGVAALAALAAGVLSGLVPAL
                     AAARVDVQGSLKEGARGDVGDARSRRLRSALVVVEVALSLLLLVGATQTMDTFRRLAL
                     TDPGFRSRDILTLSVTLPAADYPQDSAVVRLFQTLQDRITELPGVSGVGATTILPLSW
                     YESSAGIEVEGRPLRRREDAPVVGWRRVSPGYLETLAIPLVHGRAITAADRMGALPVA
                     VVSERAARLLWPGEDAVGKRFRPDTGQWIAVVGVVRDVRGNPLMGRDVGAAVYVPNRQ
                     RPARIMSFVVRTAGDPTALARPIQHAITALDSRLAAGDVTPMPRVVAAALSPQSATAR
                     TLVVTALVALLMACIGTYGVMAYTVSQRTRELGVRVALGATPAGVLRLVLGGALRLVG
                     IGIVIGLAGVVAMSRGLQAILVGTRATDPLTLSAVALALAAVTAASSWVPARRATRVD
                     PMEALRAE"
     gene            7716..>8230
                     /locus_tag="AC16JC_01765"
     CDS             7716..>8230
                     /locus_tag="AC16JC_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5163454.1"
                     /translation="MTVWYAALFTLIGSVLIVLVAWVVATRHPVAPAEQVGPLIYRVR
                     RVYFLVIVTIAVALLALTLPRTPYARSARESPAMTLTVDGVMWSWRVTGPDTSSRSAA
                     PVIVPVGKPVRFEVGALDVNHGFAVYRPDGGMLGQTQAMPGYRNVLTLTFPAPGTYRI
                     LCLEYCGLGHQT"
BASE COUNT         1212 a         2888 c         2856 g         1274 t
ORIGIN      
        1 ctcgtagtac ctcaccccct gtccccctct ccccctcgct gcgctcgagg caggctctgt
       61 cgggagaggg gaacgaagtg gaagaagacc ctgcatctag tgccccctct ccgcgtcaag
      121 cggagagggg gacaggggga gagggacaga ctgatccgcc gagcgaggct ccccgccagg
      181 ccgcacggtt catatttgac ccgtgtcccc accccgctca ctcgacacca cccgcgaagc
      241 cgacgccatt cgcttcgcgc gccggcgtgc aacgaagcct caatccggct ttgttgcggc
      301 gggtttccct gaactggacg tccggttcgc ttgaggcggg gcaaaggatg acggcagccc
      361 gcccgtcagg ctacggatga acgccgcgat ggtacgcacg tcggcgtcct cgaggcgacg
      421 gtggagttgc acgcgggcca tgacgcggat ggcttccgcc aaggtcgcca ctgagccgtc
      481 atggaagtac ggcccggtct cagccgcgtt gcgcaggctg ggcaccttga agacgtaacg
      541 gtccgcgtcc acgtgagtga cgtcgaagcg tccactgtcg acgtgggtgc tcttggtcgc
      601 aagccaataa ttctcgacga caccgaatcg ctggaaggag cctccgccca ccccgacgcc
      661 gttgtgacag ccgctgcaac cgatgtccag aaaccgtcgc aggccggcca gctcggcagc
      721 cgataacgcc gtggcatcgc cgtcgagata cgcatcgaac cgggacgggg tgagcaacgt
      781 ccgctcatat gcggccaccg cgtcggcgaa atgatgcccg ctgaccgtcg gtgtgtcccg
      841 ggggaatgcc gctgcgaact gcgtcgcgta gtcgcgcaac gcgcgcagac ggcgttcggc
      901 gtcgtcgagg gaggcgtgcc ctccgctctg gggacccgtg agggccttca acgcctgatc
      961 ttccaggtcg cgccggtcgc cgcgccagtg ctgcgcgaac tggaacgtcg cattgaggac
     1021 cgtcggcacg ttgcgcggtg tccgccgccc gagcacgccg accgaggtcg ctccagtgtc
     1081 ggctccgtaa tggctcgtcg ggtgacacgt ggcgcaactc accttcccgt ccagcgacca
     1141 gcgcgggtcg aagaacaggc gccggcccag tgcctggcga gccacgagca cggagtcccg
     1201 ggtctcgggc gccctccgga tggcctggaa ctggctgcgg gccagatgaa ggagcattgt
     1261 gtccgccggg gtttgggacg agcgtgagac cgcgccttcg acggaccacc cgcccgcacc
     1321 gatgacaccc gcccaaacga gccagactac cttgttcatt cgtcgccccc ccggacccta
     1381 cgccgatgga tatttgcccg gtgagattac tcccgcctgt gctacgacgc acctcattct
     1441 gacgaagccc gcacacggct tcggtcagga aatcgctcca gccgactcct tgagcgcacg
     1501 atccagggct cgcgccaggc gttcagccgc ctggtgaatc tgtgctgcgg aatgggccgc
     1561 ataaccgagc agcagggcgc ccggcgtcga gggccgccgc gtaccgattg taaaacggga
     1621 cagcggccac acgagaactc cctcggcagc ggccagtgcc gtggcacgcc ggtcatcgac
     1681 gcccaccggg agccggccca cgagatggag tccggctgcg tccggctcca ccgcgagcaa
     1741 cccgcccagt ttggcctcgg cggcggcgag taacgcggcc tggcgctcgg cgcagagcgt
     1801 ccgcacctgt cggatgtggc gggagtaatg cccctcgccg atgaaatccg ccaggacgcc
     1861 ctggtccgtg gtcggcgagt gacggtcggt tgcggttcgc atcgcccgga acgcatcgat
     1921 cagcgcgtca gggacgacca gaaacccgag ccgcaggctc ggggccagcg tcttgctgaa
     1981 ggtaccgaca tagatcaccc gggacgacgt gccgtcctgc gatgtatcgg tgtcgagccc
     2041 ctgcagacac ggcagctgcc tgccgccgta tcggaactct gagtcgtagt cgtcctcgac
     2101 gatccacgca gacacttggc gcgcccaggc cgagagctcg agccggcggg gggcgctcat
     2161 caacaccccg agcgggaact gatgcgaggg ggtcacgtag gccaggcgtg ccccacgcgc
     2221 cgcccgcttg ccggcagcga catccaggcc ctcctggtcc accggcaccg ctatgaggcg
     2281 ggccccagcg gtctggaaag ccatccacgc tcccacgtat ccgggattct cgatccacac
     2341 cagttcatcc gggtcgagca ggacctggcc gatcaggtgc agcgcttgtt gcgcaccatg
     2401 gaccacgatg acctggtcgg cagtgcagcg cgcacgacgg gccgctccca ggtagtccgc
     2461 aatggcgttg cgcagcgggt actcgccggc cggatccgcg tggccgagac cgaccacctg
     2521 ctggcgccag cgacgcgctg caaaccgggc ccacagacgg gtcgggaagg cgtcgacggc
     2581 cgagatcccc agctcgaacg gagccgatgt cgatccactg gtgcgcacga aaccgctgcc
     2641 actgcgcatc accagcgcgg ctcgtttcga gagccgcggc agaggttgcg ccgatcgggt
     2701 ggatccctcg gtgcggcgtt gtgcgcgcgc gtgcgtgggc gttggcggtt cgagtgccgc
     2761 atcgggcatc gtggaccgca cccgcgttcc gccaccccgc cgggtggcca ggtacccctc
     2821 gctgcggagc tgctcgaacg cttgcaggac ggtgttgcgc gcgactcgca aatccgccgc
     2881 gagcgcccgg ctggagggca gccgggcccc cgcggcgagc cgtccggaga ggatggctgc
     2941 ccgcacacca tcgtagacct gccggaacag cggcgtcgcg agcacgggat ccatgtccac
     3001 ctgcagtatc ggcgacacgg gacccggacg agcgatattt ggctccattg ttggttcgca
     3061 atttggcact tgtagtggat ccaagcaatc gccgattttg cgccatgcag cccttacatc
     3121 cgggagttgc ggtctcggcg ggtcggcgcg agtgggccag tctggcggtg atggccttgc
     3181 catgcctgct ctacgcgatg gacggtacgg tgctgaattt ggccgttcct tcgctcagcg
     3241 ccgcgctgac gccgtccagc accgagctgc tctggatcgt ggatatctac ggcttcttca
     3301 ccgcgagcgc tctcgtcacg atgggaacgc tcggggaccg gatcgggcgg cgccggctcc
     3361 tgctcatcgg ggcggcagcg ttcgggattg cgtccgttct ggccgcgttc gcgacgagcg
     3421 ccaggatgct gatcgcggcg cgcgcgatcc tcggactggc cggcgcgacc ctggccccgt
     3481 ccactctctc cctggtccgc aacatcttcc tcgacccgca ggaacggagc ctcgccatcg
     3541 gcctgtggac ggcgagcttc tcggtcggcg ccgcgatcgg tccggccatc ggcggcctgc
     3601 tgttgcagcg cttctggtgg gggtcggtct ttctcgtcag cgtgccggtc atgggtgcgt
     3661 tgctggtgct cggcccctgg ctgctcccgg aataccggga ttcccgcgcc gggcgcatgg
     3721 acttcgtgag cgcggcgttg tcgttgatcg gggtgctcgc gctgatctac ggcctcaaac
     3781 gagcggccgt ggacggggtg ggcccgctcc ctggcctgtg ggcgctggcc gggatggcgg
     3841 cctgcgtcct gttcgggtgg cgccagcgcc gagtcacgga gccgttgatc gatctcgggc
     3901 tgttccgcgc cccggccttc aacgcggcgt tggccacgaa tgccctcagc ttgttcaccg
     3961 cgttcggatc cttcctcttt accgctcagt accttcagtc ggtactcggc cttccgccgc
     4021 tcgcggcggg tctctggacc gtcccctcgt cgggcgggtc tatcgtcggc tctctgctcg
     4081 ctccgctgat cggacgccgc gttcgctccc ggacggtcat cctgggtggt ctgggactgg
     4141 cagtcatcgg ctttggcgtg ttaacgcagg tccaggacgc cgcggggctg cccctggtcg
     4201 tgacgggttc gatcaccctc gccgtgggtg tcgcagcggt ggtcaccgtg tccactgaca
     4261 ttattgtggg ggcggcgccg cccgagcggg cgggagccgc gtcggccatc tccgagaccg
     4321 gcgcggagct gggtggcgca ctcgggattg ccatcctcgg cagcatcggg acggccgtct
     4381 atcgccgagt ggtagcgtcc gcgcttccgt tcggcctctc ggaaacggca gcggacacgg
     4441 tgcggcagac agtcggcggc gcgctcgccg aggcccggcg gttgcccgaa tcgctgggtg
     4501 ccgcggtcgc cgatgtggcc cgtgcggcgt tcatcgacgc cttccgaacg acggccgcgg
     4561 tcagcgcggt gatcgcgctg tccattgccg cgttggcggc ggcgctgctg cgccgggaac
     4621 gtgcggattt gccatgatgc tgcgctgggt cgtcgcgacc gcccacctcc tcgcgctccc
     4681 gctgggcctg ggggccgtct gggcgcggag tcgcgcgttg gcctcggtcg aaagtcccac
     4741 gggccttggg agcgccttcg ccgccgacac ggtgtgggga ctggcggctg cgttgtggat
     4801 cacgacgggg ctggcacgtg cgttcggggg gctggagaag ggcaccgact actacctgca
     4861 cgatcgggtg ttctacgtga agatgggact ctttctggtc gttctgctcc tggagatgcg
     4921 gccgatgatc acgctgctac gctggcgcag caccgtccgg cgcggggaca gtccagatac
     4981 gacggctgcg ccggcactcg cccgcatcag tcgaattcaa gccgggctgg tcatcataat
     5041 ggtgtttgcg gcaaccgcgc tcgcgcgcgg ctggtgattc tcccttcggg ttccagcgtc
     5101 acggctgcgg gaagtggaag gacgacggga gcgggcggtg cgacttcgac atgagcgggt
     5161 gatgctattc ggccctcaag gcctccatcg gatccacccg cgtcgcccgg cgcgcgggga
     5221 cccagctgga agcggcggtg acagccgcga gcgcgagtgc caccgcggaa agcgtgagcg
     5281 gatcggtggc cctggtgccc accaggatcg cctggaggcc gcggctcatc gccaccacac
     5341 ccgcgaggcc gatcacgatg ccgatgccca cgagccgcag cgccccgccg aggaccagcc
     5401 ggagcacgcc ggccggagtg gcgccgaggg cgacacgcac gccgagctcc cgggtgcgct
     5461 gggagacggt gtaggccatc acaccgtagg tgccgatgca cgccatgagg agggcgacca
     5521 gcgccgtcac caccagggtg cgcgccgtcg cgctctgcgg ggacaacgcc gccgcgacca
     5581 cgcgcggcat cggggtgacg tcgccggcgg caagacggga atcgagcgcg gtgatggcgt
     5641 gctggatcgg ccgcgcgagc gcggtgggat cgcccgccgt gcgcacgacg aagctcatga
     5701 tgcgcgctgg gcgctgtcgg ttcggcacgt agacggccgc tcccacgtcc cggcccatca
     5761 ggggattgcc gcgcacgtcg cgcaccaccc ccacgaccgc gatccactgc ccggtgtcgg
     5821 gccggaatcg ttttccgacc gcgtcctccc ccggccagag cagccgggcg gcccgctcgc
     5881 tcacgaccgc cacgggcagg gcgcccatcc ggtccgccgc ggtgatggcg cgtccgtgca
     5941 ccagtgggat ggccagcgtc tcgagatagc ccggcgagac gcgccgccac cccacgacgg
     6001 gcgcatcctc gcgccgccgc agcggccggc cctcgacctc gattccggcg ctgctctcgt
     6061 accaggacaa tggcaggatc gtcgtcgcgc ccacgccgct gaccccgggc agctcggtga
     6121 tgcgatcctg aagcgtctgg aacagccgca cgaccgcgct gtcctgcgga tagtccgcgg
     6181 ccggaagggt gacgctcagg gtcaggatgt cccggctgcg gaaccccggg tcggtgagcg
     6241 ccaggcggcg gaaggtatcc atcgtctggg tcgcgcccac cagcagcagc agcgacaggg
     6301 cgacttccac cacgaccagc gcgctgcgca gtcgccggga gcgcgcgtcc ccgacatcgc
     6361 cccgcgcgcc ttccttgagt gatccctgga cgtccacgcg cgcggcggcg agtgcgggaa
     6421 cgagaccgga caacaccccg gcggcgagcg cggcgagcgc cgccacgccc agggcccgcg
     6481 gatccagtcg gagctcggcg aaacccggga tgaacgcgcg cacctcgacc gggacgctgg
     6541 tggccatcgc gcgcagtccc cccagcgtgc cgaagatgcc gagcacgcca ccggccgcgc
     6601 cgatcagcag gctctcggcc agttgctgac gcacgagccg cgcgtagggc gcgcccatcg
     6661 cgattctgac tgccaactcg cgccgccggc ccgtcgcacg ggcgaagagc aggttggcca
     6721 cattggcgca ggcgatgagc agcacgaacg cgccggcggc caggagcacc atcatgaagg
     6781 gccgcgggcc ctgcccgtag tactgctcgg caggctcggc gcgcatcatc caaccggcat
     6841 tcgtcgcagg gaagtcagcg gcgagacgcg cgcccagggc ggcgactgcc gcctccgcac
     6901 cggacggtga gacgccgcgg gcgagacgcc ccagcacgaa gacgccctgg ccgcgccgca
     6961 cggcccacga ctcgggagag agcgccaggg gcgcgagcag ctccgtgccg gcggggaacg
     7021 tgaagtcttg cggcaggatg ccgacgaccg tgtactcctc cccattgatc cgcacggtcc
     7081 gtcccaccag ggccgtgtcg ccgccgaagc gccgttgcca gagtccgtgg gagaggatga
     7141 ccacgtgacc cgcgccccac tcgtcgtccg cctcggtgaa atcccgcccc agcgccgggg
     7201 cgcgaccgat cgtacgcagg aagttcgcgg acaccagcgc tgcgccgacg cgctcgggga
     7261 cgtcgatgcc ggagatgttc gcgctccagc cgcgctgcgc ggcgagagcg ctgaactctg
     7321 gcagcgcacg cgcagcgcga tacgtcgcag gcgccatgcc gtcgctgccg cgcggcgcat
     7381 cggcgggagc ctcccacacg tgcatcacac ggcccgcgtc gcgcacctgg ggcagcggcc
     7441 ggaacgagaa tgtctcggcg gtgctgaaca tgaccgtcac cgacgcgatg cccagaccca
     7501 tgcagatgat ggcgaggagc gccagtccgg gcgttttccg aagcccccgg acggcgtagc
     7561 ggaagtccag caggacggag ttcatcgagt ccccagtacg tgcgcagcgg ctcggttgtg
     7621 tcgggggtcc ggcgtcttgc caccagcggg ttttcctgta ctctagcaag cggaaggtcc
     7681 gccctcctcg gtctcacttt ccccaggagc gttccgtgac tgtgtggtac gcggctctgt
     7741 tcaccctgat cggctcggtg ctcatcgtcc tcgtcgcctg ggtcgtcgcg acccgccatc
     7801 cggtggctcc ggccgagcag gtgggaccgc tgatctaccg cgtccggcgg gtctacttct
     7861 tggtgatcgt cacgatcgcc gtcgcactac tggcgctgac gctcccccga acgccgtacg
     7921 cccgcagcgc gcgcgagtcg ccggccatga cgctcacggt cgacggcgtc atgtggtcgt
     7981 ggcgcgtgac gggaccggac acctcatccc gatcggccgc cccggtgatc gttcccgtcg
     8041 gcaagccggt gcgcttcgag gtcggcgcgc tcgacgtgaa ccacggattc gccgtctacc
     8101 ggcccgatgg ggggatgctc ggccagaccc aggccatgcc aggctaccgc aacgtcctga
     8161 ccctgacctt cccggccccc ggcacctacc ggatcctgtg cctcgagtac tgtgggctgg
     8221 gtcatcagac
//
LOCUS       DBNGZW010000069         4653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1026598, whole genome shotgun sequence.
ACCESSION   DBNGZW010000069 DBNGZW010000000
VERSION     DBNGZW010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1026598"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="AC16JC_01770"
     CDS             complement(<1..1310)
                     /locus_tag="AC16JC_01770"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000225 -
                     N-acetylglucosaminylphosphatidylinositol deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006506 - GPI anchor biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HIR5163455.1"
                     /translation="MRRVLTALAGFALGAAGSGWAQQLAPAGTGGVAALANALEQLGS
                     TKRVLVIGAHPDDEDTQLLTWLSRGLGAQAAYLALNRGEGGQNLIGPELGPELGIIRS
                     EELLGARAVDGARQFFTRAYDFGFSKSADETFRFWSRDTVLRDVLDVIRRFRPQVIVS
                     IFSGTPRDGHGHHQVAGILARQAFDLVKDSSWGPVKFYRSTFFDTASTTLTIPTGGLD
                     PILGQSYYQIAMAGRSRHRSQDMGQLQRPGPAQTRLALLESRVGDAPGVFAGVDTVWH
                     GRERFRALIDSARARLNPYAPDGIAPFLARALSLLGPEDGEQRAILEGALATAANVVV
                     DGVADDGIVIPGERMQVEVSVWNAGPAPVPVDAIEFAAPAGWRIDQGEPATGTVAPNT
                     LLTRRFEVTVAPEAPRSQPYFLRRPQLGAMYDWSGVPAEVRGLP"
     gene            1590..3461
                     /locus_tag="AC16JC_01775"
     CDS             1590..3461
                     /locus_tag="AC16JC_01775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5163456.1"
                     /translation="MTTTAAEPKPKVSADEARAVAEAAREEQWAAPSFVRDLFLGKLR
                     MDLIHPYPLQDPQEAERARPFLEQLTRLLSQVDSDRIDREGEIPPAVVQGLKDIGAFG
                     IKIPREYGGLGLSQLSYMKAIELVSSVDGSITALLSAHQSIGVPQPLKMFGTDEQKKK
                     FFPRLAKGAVSAFALTEPDVGSDPAALETTAVLSPDGREWVLNGSKLWCTNGTIADLM
                     VVMARTPSKTVGGKEKRQITAFIVEANTPGVTVDHRCHFMGLKAIYNGVVSFTNVRVP
                     AENILWGEGKGLKLALITLNTGRLTLPISAVAAGKRCLEISRAWANERVQWGKKIGQH
                     DAVAQKIGTMVANTFAMEAVAELCGALADGGTSDIRLEAAIAKLYNSEAGWRIIDDTL
                     QVRGGRGYETADSLRDRGEKAVPVERIMRDFRINLIFEGSSEIMHLFIAREAVDKHLQ
                     VAGDVVMPGKSAGQRLAGLVRSALFYGWWYPSRWLGWSFWPKYGEFGELARHVRFVER
                     SARRLARGVFHAMMRFGPRLEQRQGVLFRLVDVGAELFAMAATCSRAARLLETDPTAG
                     AHAMRVADVFCRQARGRVKAKFQGLWRNEDVPTYKVAQEVLRGEHAWLERGMVDVKY"
     gene            complement(3565..3936)
                     /locus_tag="AC16JC_01780"
     CDS             complement(3565..3936)
                     /locus_tag="AC16JC_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5163457.1"
                     /translation="MMPFERLEAWRACHLLALATYRVTAGFPDTERYGLTVQMRRAAV
                     SAASNIAEGSAKRGARELRRYLDIALGSLAELCYQIRLSKDLAYISVDQFAELEAQRS
                     TAGKLTWGLHESVRRREALSG"
     gene            complement(4069..>4653)
                     /locus_tag="AC16JC_01785"
     CDS             complement(4069..>4653)
                     /locus_tag="AC16JC_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163458.1"
                     /translation="ASQGYVHCLLLGVTPEGVEIAANAAKAMGASFDVLVAAFIRLGD
                     NLAPVGALAENTTSAEMDPDFQPGMALVEKLSSAIEESRARVRRDLVLYRTNRPIKRL
                     DDRQVIIVDGPVFYPWKVLAAARAVQELGARRIAIATPVATTDAAIRVRARRFEFVCP
                     NVLEAVEQNTRLYGDAMGESPERLKSILIAHQAA"
BASE COUNT          765 a         1572 c         1587 g          729 t
ORIGIN      
        1 aagggcaggc cgcgaacctc ggcgggcacg ccgctccagt cgtacatcgc acccagctgc
       61 ggccggcgca ggaagtaggg ctgtgaccgc ggcgcctccg gtgccacggt cacttcgaat
      121 cgtcgcgtca acagggtgtt gggggcgacc gttcccgtgg ccggctcccc ctggtcgata
      181 cgccagcccg cgggcgccgc gaactcgatg gcgtccaccg ggacgggcgc ggggccggcg
      241 ttccagacgg acacctcgac ctgcatccgc tcacccggta tcacgatccc gtcgtcggcg
      301 acgccgtcca ccacgacgtt ggcggcggtg gcgagggcgc cttcgagaat cgcccgctgc
      361 tcgccgtcct cgggacccag gagcgacagc gcacgcgcca ggaacggcgc gatcccgtcg
      421 ggggcatagg gattgagccg cgcgcgcgcg gagtcgatca gcgcgcggaa ccgctcgcgg
      481 ccgtgccaga cggtgtcgac cccggcgaag acgcccggcg cgtcgcccac ccgcgactcg
      541 agcagggcga gccgcgtctg ggccggcccg ggccgctgca gctggcccat gtcctgggac
      601 cggtgccggc tgcggccggc catggcgatc tggtagtagg actggccgag gatgggatcg
      661 aggccaccgg tgggaatcgt cagcgtcgtg gacgccgtat cgaagaaggt ggagcggtag
      721 aacttgaccg gtccccagct cgagtccttc accagatcga aggcctgccg cgcgaggatt
      781 cccgccacct ggtggtggcc gtgcccgtcc cggggcgtgc ccgagaagat cgagacgatg
      841 acctggggcc ggaaccggcg gatcacgtcc agcacgtcgc gcaacaccgt atcgcgcgac
      901 cagaagcgga atgtttcgtc ggcggacttg gagaagccga agtcgtaggc gcgggtgaag
      961 aactgccgcg ctccgtcgac cgcgcgcgcg cccagcagct cctcggaccg gatgatgccc
     1021 agctccggcc ccagctcggg gccgatcagg ttctgcccgc cttccccgcg gttcagtgcg
     1081 aggtaggccg cctgggcgcc gaggccgcgg gacagccaag tgaggagctg ggtgtcctcg
     1141 tcgtcggggt gggcgccgat gaccagcacc cgcttggtgc tccccagctg ctccaacgcg
     1201 ttcgccaacg ccgcgacgcc gccggttccg gcgggggcga gttgctgagc ccatccggaa
     1261 ccggcggcgc cgagcgcgaa ccctgcgagg gcggtgagga cgcgtcgcac tactgggaaa
     1321 ggtacacggt gccacccttc ataacgaacc tgacccgttg gagttccgtg atgtccttga
     1381 gcgggtcgcc gccatccaca ccactctgat ccgctggcct tcgatcagca tgcccacgtt
     1441 ggcctgtgcc tgctcggatc gtccgtcgat caagcggccc gcttctatga agacgcgcgc
     1501 ggcaggccgc gaatctctcc gggaagaccg gggtgtcaag gtgacgcgcg tccccgactt
     1561 ttaggcgtcg ccccgctaca ttatttcgca tgactaccac cgctgccgaa cccaagccca
     1621 aagtcagcgc cgacgaggcg cgcgccgtcg ccgaggcggc gcgcgaggag cagtgggcgg
     1681 cgccgagctt cgtccgggac ctgttcctcg gcaagctgcg gatggacctg atccatccgt
     1741 atcccctgca ggacccgcag gaagccgagc gggcccggcc gttcctggag cagctcaccc
     1801 ggctcctgtc gcaggtggat tcggaccgca tcgaccgcga aggcgagatc ccgcccgccg
     1861 tggtccaggg cctgaaggac atcggcgcgt tcggcatcaa gatcccgcgc gagtacggcg
     1921 gtctggggct gtcgcagctc tcctacatga aggcgatcga gctggtgtcg tccgtggacg
     1981 gctcgatcac cgcgctgctc tcggcccacc agtcgatcgg cgtgccgcag cccctcaaga
     2041 tgttcggcac cgacgaacag aagaagaagt tcttcccgcg actcgcaaag ggggcggtgt
     2101 cggcgttcgc gctcaccgag cccgatgtcg gctccgatcc cgcggcgctc gagacgacgg
     2161 ccgtcctctc ccccgacggg cgcgagtggg tgctgaacgg ctcgaagctg tggtgcacca
     2221 acggcacgat cgccgacctg atggtggtga tggcgcgcac gccgtcgaaa accgtcggcg
     2281 gcaaggaaaa gcggcagatc acggcgttca tcgtcgaagc gaacaccccg ggcgtaacgg
     2341 tggaccatcg ctgccacttc atgggactga aggcgatcta caacggcgtg gtgtccttca
     2401 ccaacgtccg cgttcccgcc gagaacatcc tctggggtga gggcaaaggc ctgaagctcg
     2461 cgctcatcac cttgaacacg ggccgcctca cgctgcccat ttccgccgtc gccgccggca
     2521 agcgctgcct cgagatctcc cgcgcctggg cgaacgagcg cgtgcagtgg ggcaagaaga
     2581 tcggccagca cgacgccgtg gcgcagaaga tcggcaccat ggtcgcgaac acgttcgcga
     2641 tggaggcggt ggccgagctg tgcggcgcgc tggcggacgg cggcacgtcc gacatccggc
     2701 tcgaagcggc gatcgccaag ctgtacaact ccgaggccgg ttggcgcatc atcgacgata
     2761 cgctgcaggt ccggggcggc cgcggctacg agaccgccga cagcctgcgc gaccgcggcg
     2821 agaaggccgt cccggtcgag cgcatcatgc gcgacttccg catcaatctg atcttcgagg
     2881 ggtcctccga gatcatgcac ctgttcatcg cgcgggaggc ggtcgacaag cacctccagg
     2941 tggcgggcga cgtcgtgatg cccggcaagt ccgccggtca gcggctcgcc ggtctcgtcc
     3001 gttccgcgct gttctacggc tggtggtacc cgtcgcgctg gctcggctgg agtttctggc
     3061 ccaagtacgg ggaattcggc gagctggcca gacacgtgcg cttcgtcgag cgcagcgccc
     3121 ggcgcctggc acgcggcgtg ttccatgcga tgatgcgctt cggcccgagg ctcgagcagc
     3181 ggcagggcgt gctgttccgc ctggtggacg tgggcgccga gctgttcgcc atggcggcga
     3241 cctgctcgcg cgcggcgcgg cttctcgaga ccgaccccac cgccggggcg cacgccatgc
     3301 gcgtcgccga cgtgttctgc cgccaggcgc gcgggcgggt gaaggccaag ttccagggac
     3361 tgtggcgcaa cgaggacgtg ccgacctaca aggtcgcgca ggaagtgctg cgcggtgagc
     3421 atgcgtggct ggaacggggg atggtggatg tgaagtacta ggaaagttgg aaagtgggaa
     3481 agttggaaag ttggaaagtt ggaaggttgg aaggttggta ggtgggaagg cgggaaagtc
     3541 ggaagggtgg attggcaggt ggagctagcc tgacaacgct tctcgacgtc gaaccgactc
     3601 gtggagcccc caggtgagct tacccgcggt actccgttgg gcttcgagtt cggcgaattg
     3661 gtcaaccgag atataagcga gatctttcga caaccggatt tgatagcaca gttcggccag
     3721 ggagccaagg gcgatatcga ggtaccgtcg cagctctctg gcgccacgct tcgcgcttcc
     3781 ctcggcaata ttcgatgctg ccgacacggc ggcacgccgc atctgcactg tcaatccata
     3841 ccgttcggta tcagggaacc ccgcggtcac tcgatacgtc gcgagagcca acaggtgaca
     3901 agcccgccac gcctcgaggc gctcgaacgg catcatcggt tgatggtaac aaactcagaa
     3961 acagcgggat aaccaatccg acgcaacacc tacccaattc gcgcgcgccc tcccgtctac
     4021 ctcccgtcta ccttccgtcc ttcccacctt ccatccttcc atccttcctc acgccgcctg
     4081 gtgcgcgatc agaatgctct tcagccgctc cgggctctcc cccatcgcgt cgccgtagag
     4141 cctcgtgttc tgttccacag cctccagcac attggggcac acgaactcga agcgtcgcgc
     4201 ccggacccga atggccgcat cggtggtcgc gaccggggtg gcgatcgcga tgcgacgggc
     4261 gccgagctcc tgcaccgcgc gcgccgccgc cagcaccttc cagggataga agaccggccc
     4321 gtccacgatg atgacctgcc ggtcgtcgag gcgcttgatg ggccgattgg tgcggtacag
     4381 taccaggtcg cgccgcaccc gcgcccgcga ttcctcgatc gccgagctga gcttctcgac
     4441 gagggccatg ccgggctgga agtcggggtc catctcggcc gatgtcgtgt tttcggccag
     4501 cgcgcccacg ggcgccaggt tgtcgcccag acggatgaac gccgccacca gcacgtcgaa
     4561 gctcgcgccc atcgctttgg cggcgttggc cgcgatctcc actccctcgg gggtgacccc
     4621 cagcagcagg cagtggacgt atccctggga cgc
//
LOCUS       DBNGZW010000070         7429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_413455, whole genome shotgun sequence.
ACCESSION   DBNGZW010000070 DBNGZW010000000
VERSION     DBNGZW010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7429
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_413455"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..1567
                     /locus_tag="AC16JC_01790"
     CDS             134..1567
                     /locus_tag="AC16JC_01790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016863414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5163459.1"
                     /translation="MKTDLFINNEWRPAVSGKRFPVENPATEETIAEVALGDVADIDN
                     AVGAARACFDSDAWRGLSARRRGRMLTKAADLLEARLDEFARLETAHNGKTLFESKIE
                     LTMTVNTLRYYGGWADKITGDTLPVDGPFFCYTLREPVGVVGAIVPWNFPLNIASWKF
                     SPALAAGCTVVLKPASETPLTALAMAELILEAGFPPGAFNVVPGGGSTAGAALVRHPD
                     VDKISFTGSTKVGRQIMKMAADSNKRVTLELGGKSPNIVFADADIPAAVKGAQTGIFY
                     GKGEVCAAGSRLLVEESVHDQFVELLAAGAKKLTPGDPLDKNTRLGALVSRKQRETVL
                     SYIEKGVAEGAAVVAGGKAVQPNGKGYFVEATVFDGVTPDMTIAREEIFGPVLAVLTF
                     KGTDEAIALGNLSMYGLAAGIWTKDIQKAHRTARAIRAGTVWINSYNFYDSGAPFGGF
                     KASGFGRDLGRDALDGYLETKTVWVAL"
     gene            1616..2800
                     /locus_tag="AC16JC_01795"
     CDS             1616..2800
                     /locus_tag="AC16JC_01795"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005830725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR5163460.1"
                     /translation="MSREAYIYAAVRTPVGKHGGSLAAVRPDDLAAIPIKAVVERTKV
                     DPLAIDDVILGCANQAGEDNRNVARMALLLAGLPVEVPGQTVNRLCGSGLQAVASSAH
                     AIKAGEGDVFIAGGVESMTRAPYIMLKSGEVWNRRAPEIADSTVGWRFTNPRMKKEWT
                     ISLGETAEVVAERYGITRAEQDAFAVESQQRAERAQRECVFAEELVPVPLPDGTTFAK
                     DEYPRSGTTREGVARLKPAFKKDGTVTAASSSGINDGSAALLVGSADGRMGGSKPMAR
                     IVSAAVAGVDPSCMGLGPIPATRKALQRAGLTIDQIDLVELNEAFAAQAIACIRELEL
                     DPAKVNIYGGGIALGHPLGCTGAKILTTLLYALRRTDSRYGLVTMCIGVGQGIALIVE
                     RT"
     gene            2797..3543
                     /locus_tag="AC16JC_01800"
     CDS             2797..3543
                     /locus_tag="AC16JC_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5163461.1"
                     /translation="MSLVLVEVAAGIGTVTLNRPDKLNAFDRHACREAIGALQSLTAD
                     PGVRVLILTGAGRAFSAGADLNVLGEDGPALVAAGKDLALLLRAAPQPVIAAVNGAAA
                     GGGANLALACDYRIAAEGAAIGQVFARLGLGPDWGGSYFLPRLVGPSRALELVWSARM
                     VPAAEALALGLFDRVVPADRLLAEARALAAVWAGFPALALRRAKEVIYQSERASLVEM
                     LDAEITVQHELFATPEAQSRIGASLATRSR"
     gene            3547..4668
                     /locus_tag="AC16JC_01805"
     CDS             3547..4668
                     /locus_tag="AC16JC_01805"
                     /inference="COORDINATES: protein motif:HMM:NF016993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HIR5163462.1"
                     /translation="MVRKLNNFDDWIDYFRYWQDSIGLPPGALRDFKFDAKFGDQEVP
                     HIEFGHYKGSHKWPTVMHIPDQRIRDALLNLIVYQGDTEFASVEQQRNLLHNSPSDYD
                     LLSLQRVMTEEMRHGWQMSYLLCAHFGDEGKREAAKLLERRADEGERLLGSFNEIVDN
                     WLDFFTYTQFIDSDGKFQLKMLSVSAFDPLARSMGPMLKEESFHLGTGNNGLLRIVKA
                     GKMPPEVIQRFFNKWIPTAFDLFGTDNSSSAHWAYVWGLKGRYDEREQEIEPDKSKLN
                     ELSRGLYRQEIVKLVERLNLFITERERWLHVPDLKFNRRIGMYAHQKYTVEGEPISPE
                     RYPAYLESVLPRAEDIALVHDIEKEPDWIEPKKADSLLQ"
     gene            4665..5354
                     /locus_tag="AC16JC_01810"
     CDS             4665..5354
                     /locus_tag="AC16JC_01810"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR5163463.1"
                     /translation="MSRPLVIAHRGASAVEYENSLAAFRTAGRLGADGVELDIHASAD
                     GALIVHHDAWVGGVHIPSAPAEQVRAQRLPNGEPPPLLKEALAAIDPRLRVYVEIKTL
                     PAPYDARLIGALDAGPAPSGYAVHAFDHRILARLGRVRPGLPRGVLSASYPLRPLVPL
                     TDAGAVAFWQERSLVDLELAAMLHLAGAQLIVWTVDDPSEMRRLAGLGADGLCTNRPD
                     VGRRTLDALPV"
     gene            5351..6223
                     /locus_tag="AC16JC_01815"
     CDS             5351..6223
                     /locus_tag="AC16JC_01815"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006974901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5163464.1"
                     /translation="MTAPTAPAARVAVIGAGTMGHGIAYVAALAGCRVSLHDSAADAL
                     ADARHRIESLAAAGLQRGKITEADRAAVHERLRYCGDLDDAVMDADVVIEAVAERLNT
                     KQLLFAAVERQAPREALLATNTSSLAVGQIAAAVREPARLIGMHFFNPVHLMRLVEIV
                     RHGRTPADTVDRAIAFAHRLGKEPIVVQDSPGFASSRLGVVLGLEAMRMLEQGVASAE
                     DIDKAMELGYNHPMGPLKLTDLVGLDVRLAIAEYLHQQLRMPHYAPPQILKDKVARGE
                     LGKKTGKGFYTWSE"
     gene            6220..7074
                     /locus_tag="AC16JC_01820"
     CDS             6220..7074
                     /locus_tag="AC16JC_01820"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5163465.1"
                     /translation="MNRITDVHIHIQPWRDLKPKVQAVMRAGKEDHWDFLLSLMDDPK
                     TLLEVMDRAGVWRVGLVNYPSPDIMGFTDSTNDFAARYASANPERLLPYGGVHPRFTA
                     DPAAGVDRLLELGIRMLKIHPNHQAFPANAYTTGLDALAVIYRRCEERGLPVMVHTGT
                     SIFPGARSKWGNPLELDDVAIDFPDLRIVMAHGGRPLYMEEAFFILRRHRNVRLDVSG
                     IPPLKLLDYFPRLAEIGARVLWGTDWPSPGVRDLRTNIDQFLTLPLADELRRAILETN
                     ALALFPAS"
     gene            7158..>7429
                     /locus_tag="AC16JC_01825"
     CDS             7158..>7429
                     /locus_tag="AC16JC_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008745234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5163466.1"
                     /translation="MGASPLDAQAGRISYDTFSLANGLRVIASEDHSTPIVAVDVWYN
                     AGSRNERAGRSGFAHLFEHMMFQGSAHVKKAEHFQRVQRAGGTDNG"
BASE COUNT         1222 a         2529 c         2572 g         1106 t
ORIGIN      
        1 cgggcgccga ggtccccttc gagtccggcc tcgccatcga gcgggagctg cagcagcagc
       61 tgttccagag cgaggacgcc aaggagggca tcgccgccta caccgagaag cggatggcgg
      121 agttcaaggg ccggtgaaga ccgacctgtt catcaacaac gagtggcgcc cggccgtctc
      181 cggcaagcgt tttccggtgg agaatcccgc cacagaggag acgatcgccg aggtcgcgct
      241 gggggacgtc gccgacatcg acaacgccgt gggtgcggcg cgggcgtgct tcgattccga
      301 cgcctggcgc ggactctcgg cgcgcaggcg cggccgcatg ctcaccaagg cggccgacct
      361 gctcgaggcc cggctggacg agttcgcccg gctcgagacg gcgcacaacg gcaagacgct
      421 gttcgagtcg aagatcgagc tcaccatgac ggtcaatacg ctgcgctact acggcggctg
      481 ggccgacaag atcacgggcg acacgctgcc cgtggacggg ccgttcttct gctacacgct
      541 gcgcgagccc gtgggcgtgg tgggcgccat cgtcccgtgg aacttcccgc tcaacatcgc
      601 gagctggaag ttctcgccgg cgctcgcggc cgggtgcacc gtggtgttga agcccgcgtc
      661 ggagacgccc ctcacggcgc tggccatggc cgagctgatc ctcgaggcgg gcttcccgcc
      721 gggggccttc aacgtcgtcc cgggcggcgg atcgaccgcc ggagcggcgc tggtgcggca
      781 ccccgacgtg gacaagatct cgttcacggg atcgaccaag gtcggccgcc agatcatgaa
      841 aatggccgcc gactcgaaca agcgcgtcac gctcgagctg ggcggcaaga gcccgaacat
      901 cgtcttcgcc gacgcggaca tccccgcggc cgtcaagggg gcgcagaccg ggatcttcta
      961 cggcaagggt gaagtgtgcg ccgccggttc ccggctgctg gtggaagaga gcgtccacga
     1021 ccagttcgtc gagctgctgg ccgccggcgc caagaagctc acgcccggcg acccgctcga
     1081 caagaacacg cgcctgggcg cgctggtctc ccggaagcag cgggagacgg tgctctccta
     1141 catcgagaaa ggcgtcgcgg aaggcgccgc ggtcgtcgcc ggcggaaagg cggtccagcc
     1201 caacgggaag ggctacttcg tggaagccac cgtgttcgac ggcgtgacgc ccgatatgac
     1261 gatcgcccgg gaagagatct tcgggccggt gctcgccgtg ctcaccttca agggcacgga
     1321 cgaggcgatc gcgctgggca acctctcgat gtacggcctt gcggcgggca tctggacgaa
     1381 ggacatccag aaggcgcatc gcacggcccg cgccatccgc gccgggactg tgtggatcaa
     1441 cagctacaac ttctacgact ccggcgcgcc gttcggcgga ttcaaggcct ccggctttgg
     1501 acgggacctg ggacgcgatg cgctggacgg atatctggaa accaagaccg tttgggtggc
     1561 gctgtgaggg cggaagaggg cggacgaagg cggacacggg cggacaacga cgagcatgag
     1621 tagagaagcg tacatctacg cggcagtacg gacacctgtg ggaaaacacg ggggctcgct
     1681 cgcggccgta cgccccgatg acctcgcggc gatcccgatc aaagcggtgg tcgaacggac
     1741 caaggtggac ccgctcgcga tcgacgacgt gatcctgggg tgcgcgaatc aggcgggaga
     1801 agacaaccgc aacgtggcgc ggatggcgct gctcctcgcc gggctgcccg tcgaggtccc
     1861 gggccagacc gtcaaccggc tgtgcggctc cggactgcag gcggtggcca gctcggcgca
     1921 cgcgatcaag gcgggcgagg gcgacgtctt cattgcgggc ggcgtggaga gcatgacgcg
     1981 cgcgccttac atcatgctga agtccggcga ggtgtggaac cgccgcgcac ccgagatcgc
     2041 cgattcgacg gtcgggtggc ggttcacgaa cccgcgcatg aagaaagaat ggacaatctc
     2101 gctcggcgag acggcggagg tcgtggccga gcgctacggc atcacccgtg ccgagcagga
     2161 tgccttcgcc gtcgagagcc agcagcgggc cgagcgggcg cagcgcgaat gcgtgttcgc
     2221 ggaggagctc gtcccggtcc ccctccccga cggaacaacg ttcgccaagg acgagtaccc
     2281 gcgttccgga acgacccggg agggcgtagc gcgcctcaag cccgcgttca agaaagacgg
     2341 cacggtgacc gcggccagct cgagcggcat caacgacggg tcggcggcgc tgctggtcgg
     2401 atcggcggat ggacggatgg gcggatcgaa gcccatggcc aggatcgtga gcgcggcggt
     2461 cgccggcgtg gatccgagct gcatgggact cggccccatc cccgcaaccc gcaaagctct
     2521 gcagcgcgcc gggctgacga tcgatcagat cgatctggtc gagctgaacg aagcgttcgc
     2581 cgcgcaggcg atcgcgtgca tccgggagct cgagctcgat ccggcgaagg tgaacatcta
     2641 cggcggcggg atcgcgctgg gacaccccct cgggtgcacc ggcgcgaaga ttctcacgac
     2701 gctgctctac gccttgcgcc gtaccgattc ccgctacgga ttggtcacga tgtgcatcgg
     2761 ggtcggccag ggcatcgcgc tgatcgtgga gcgcacatga gtctcgttct ggtggaggtc
     2821 gccgcgggca tcgggacggt gacgctgaac cggcccgaca agctgaacgc gttcgaccgg
     2881 cacgcctgcc gcgaggcgat cggggcgctc cagtcgctga ccgccgaccc gggcgtgcgc
     2941 gtgctgattc tgaccggcgc cggccgcgcc ttctcggccg gggccgatct caacgtgctc
     3001 ggtgaggacg ggccggcgct cgtcgcggct gggaaggacc tcgccctgct gctgcgcgcg
     3061 gcaccgcagc cggtgatcgc ggcggtaaac ggtgcggctg cgggcggcgg cgccaatctc
     3121 gccctcgcct gcgactaccg gatcgcggcc gagggcgccg cgatcgggca ggtgttcgcc
     3181 aggctcggcc tcggtcccga ctggggcggg agctatttcc tgccgcgact ggtggggccg
     3241 tcgcgggctc tcgagctggt ctggagcgcc cgcatggtgc ctgcggcgga ggcgctcgcg
     3301 cttggcctgt tcgatcgcgt cgttcccgcc gaccgcctcc tggccgaagc gcgcgccctg
     3361 gccgcggtgt gggcgggttt tccggcgctc gcgctgcggc gcgccaagga agtgatctat
     3421 cagagcgagc gtgcgtcgct cgtcgagatg ctggacgcgg aaatcacggt gcaacacgag
     3481 ctgttcgcca cgcccgaggc gcagtcgcgc atcggtgcct cactcgccac gcggagccgc
     3541 tgagccatgg tacggaagct gaacaacttc gacgactgga tcgactattt ccgctactgg
     3601 caggactcga tcggactgcc gccgggggcc ctgcgcgact tcaagttcga cgccaagttc
     3661 ggcgaccagg aagtcccgca catcgagttc ggccactaca agggcagtca caagtggccc
     3721 acggtgatgc acatccccga tcagcgcatc cgcgacgcgc tgctgaacct gatcgtgtac
     3781 cagggcgaca ccgagttcgc ctcggtggag cagcaacgta acctgctgca caattcgccc
     3841 tcggactacg atctcctgtc gctgcaacgc gtgatgaccg aggagatgcg gcacggctgg
     3901 cagatgtcct acctgctgtg cgcgcatttc ggcgacgagg gcaagcgcga ggccgcgaag
     3961 ctgctggagc gccgcgccga cgagggcgaa cggctgctcg gcagcttcaa cgagatcgtg
     4021 gacaactggc tcgacttctt cacgtacacc cagttcatcg acagcgacgg caagttccag
     4081 ctgaagatgc tctcggtgag cgccttcgat ccgctggcgc gctcgatggg gccgatgctc
     4141 aaggaggagt ccttccacct gggcaccggg aacaacgggt tgctgcgcat cgtgaaagcc
     4201 ggcaaaatgc cgcccgaggt gatccagcgg ttcttcaaca agtggatccc gacggcattc
     4261 gatctgttcg gcaccgacaa ctcctcctcc gcgcactggg cctacgtgtg gggtctgaag
     4321 gggcgctacg acgagcgcga gcaggagatc gagcccgaca aatcgaagct gaacgagctc
     4381 tcccggggcc tgtaccggca ggagatcgtg aagctcgtgg agcggctgaa cctgttcatc
     4441 accgagcggg agcgctggct gcacgtcccc gacctgaagt tcaaccgccg catcggcatg
     4501 tacgcccacc agaagtacac ggtggagggc gagccgatca gcccggagcg ctacccggcg
     4561 tacctggaga gcgtgctccc gcgggccgaa gacatcgcgc tggtccatga catcgagaag
     4621 gagccggact ggatcgagcc gaagaaggcg gatagcctgc tccagtgagc aggccgctcg
     4681 tcatcgccca ccgcggcgcc tccgcggtcg agtacgagaa ttcgctcgcg gccttccgca
     4741 ccgcgggccg cctgggcgcg gacggcgtcg agttggacat ccacgccagc gccgacggcg
     4801 ccctcatcgt gcatcacgac gcgtgggtcg gcggggtgca catcccgagc gcccccgccg
     4861 agcaggtgcg cgcgcagcgc ctgcccaacg gtgagccgcc tcccctgctc aaggaggcgc
     4921 tggcggccat cgatccccgg ctgcgcgtgt acgtggagat caagacactg cccgcaccct
     4981 acgacgcgcg tctgatcggc gcgctggacg ccggccccgc tccgtcggga tatgcggtcc
     5041 acgccttcga tcaccgcatt ctggcgcggc tgggccgggt gcgccccggt ctgccgcgcg
     5101 gcgtgctgag cgcgagctac ccgctccggc cgctggtgcc gctcaccgat gcgggcgccg
     5161 tggcgttctg gcaggagcgc tcgctggtgg acctggagct cgcagcgatg ctccacctgg
     5221 cgggcgccca gctgatcgtg tggacggtgg acgatccgtc cgagatgcgc cgcctcgccg
     5281 gcctgggcgc cgacggtctc tgcaccaatc gtcccgacgt gggccggcgc accctggacg
     5341 cccttcccgt atgaccgcgc ccacggctcc cgcggcgcgc gtcgccgtga tcggcgcggg
     5401 cacgatgggg cacggcatcg cctacgtcgc ggcgctcgcc ggctgccgcg tctcgctgca
     5461 cgacagcgcg gccgacgcac tcgccgacgc acggcaccgc atcgaatcgc tggccgcggc
     5521 ggggctgcaa cgcggcaaga tcaccgaggc cgaccgcgcc gccgtgcacg agcggctgcg
     5581 ctactgcggc gatctcgacg acgcggtgat ggatgccgac gtcgtgatcg aggccgtggc
     5641 cgaacgcctc aacaccaaac agctgctgtt cgccgcggtg gagcgccagg cgcccaggga
     5701 ggcgctgctg gccaccaata cgtcctccct cgcggtgggg cagatcgccg cagcggtgcg
     5761 ggagcccgcc cggctcatcg ggatgcactt cttcaacccg gtgcacctta tgcggctggt
     5821 ggagatcgtg cgccacggcc ggacccccgc cgacacggtg gaccgcgcga tcgcgttcgc
     5881 acaccggctc gggaaggagc cgatcgtcgt gcaggactcg cccggtttcg cctcgagtcg
     5941 cctgggggtc gtgctcggtc tcgaggcgat gcgcatgctc gaacagggcg tggccagcgc
     6001 cgaggacatc gacaaggcga tggaattggg ctacaaccat ccgatgggac cgctgaaact
     6061 caccgatctc gtgggcctcg acgtgcggct cgccatcgcg gagtatctac accagcagct
     6121 gcgcatgccg cactacgccc cgccgcagat cctcaaggac aaggtggccc gaggcgagct
     6181 cggcaagaag acggggaaag ggttctacac ctggagcgag tgaaccgcat caccgatgtc
     6241 cacatccaca tccagccctg gcgcgacctg aagcccaagg tccaggcggt gatgcgggcc
     6301 ggcaaggaag accactggga cttcctgctg tcgctcatgg acgaccccaa gacgctgctc
     6361 gaggtcatgg acagggccgg cgtgtggcgg gtcggcctcg tgaactaccc gagccccgac
     6421 atcatggggt tcaccgattc caccaatgac ttcgccgccc ggtacgcgag cgcgaatccg
     6481 gagcgcctcc tgccctacgg cggcgtccac ccccgcttca ctgcggatcc cgccgccggc
     6541 gtggatcggc tgctcgagct ggggatccgg atgctcaaga ttcacccgaa ccatcaggcc
     6601 tttcccgcga acgcctacac cacggggctg gacgccctgg cggtgatcta ccggcgctgc
     6661 gaggagcggg gcctccccgt catggtccac accggcacct cgatcttccc cggcgcccgg
     6721 tcgaaatggg gcaaccccct ggaactggac gatgtcgcga tcgacttccc cgacctccgg
     6781 atcgtcatgg cccacggcgg gcggccgctg tacatggagg aggcgttctt cattctgcga
     6841 cgccatcgca atgtccggct cgatgtatcc ggtattccgc ccctcaaatt gctggactac
     6901 tttccccgcc tggcggagat cggcgcccgg gtgctctggg gcacggattg gcccagtccc
     6961 ggggtgaggg atctgcggac gaacatcgac cagttcctca cgctgccgtt ggccgacgag
     7021 ctgcgccggg ccattctcga gacgaacgcg ctggcgctgt ttcccgcgtc ctgacgccgt
     7081 acacgaccca tccgtccgga gtacgatgca tgcgtgtgtt cctcctggcc gcccttagcg
     7141 gtctcgctct cacggccatg ggcgcctccc cgctcgacgc ccaggccggt cgcatcagct
     7201 acgacacctt ctccctcgcc aacggcctcc gcgtgatcgc gtccgaggac cattccaccc
     7261 cgatcgtcgc cgtggacgtg tggtacaacg ccggctcccg caacgagcgg gcggggcgct
     7321 ccgggttcgc ccacctgttc gagcacatga tgttccaggg ctcggcccac gtgaagaagg
     7381 ccgagcactt ccagcgcgtc cagcgcgccg gcgggaccga caacggcag
//
LOCUS       DBNGZW010000071         2395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_210453, whole genome shotgun sequence.
ACCESSION   DBNGZW010000071 DBNGZW010000000
VERSION     DBNGZW010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_210453"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..839
                     /locus_tag="AC16JC_01830"
     CDS             <1..839
                     /locus_tag="AC16JC_01830"
                     /inference="COORDINATES: protein motif:HMM:NF015722.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR5163467.1"
                     /translation="VTILDGSLMLSYQWRAGDATHELRLVQVPGTAGEPFAFGAGSRQ
                     RPIAIRDFHIMATPVTQALWQHIMGSNPARRPDLRCPVENVSWYDVTGPDGFVERINA
                     SAALAAVSGVAPQARFRLPSETEWEYAARGGPHWRDGFRFSGSNDAAAVAWYGRRWSG
                     VHELMVRLFGSRRGWRLANRLRFGIPRPTRTHEVATKAPNQLGIYDMSGNVWEWCQDA
                     CTDELAAVPEDGTAWPGPSPERRLRGGCHHNWDLHCTVYWRYGIEPDAHDGCIGLRLA
                     LS"
     gene            complement(869..1249)
                     /locus_tag="AC16JC_01835"
     CDS             complement(869..1249)
                     /locus_tag="AC16JC_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163468.1"
                     /translation="MTNAPLPSGRPRSVTVIGWLFIAAGIVGLVYHATALETSGPLDY
                     EAVWVLFLRLLAVIGGAFMLRGADWARWLVLAWIAYHVILSAFHSWSETAVHAALLAG
                     VAYLLLRPEAGAYFRGAKRRPEQP"
     gene            1408..2184
                     /locus_tag="AC16JC_01840"
     CDS             1408..2184
                     /locus_tag="AC16JC_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163469.1"
                     /translation="MKPTIYAPILGLVALAACSSLDTPTQPQGPAYTTYETLGDSLSI
                     ATKLDEFRAALGGVLNAPNAPPAASGRREINWDGAPAALTNVDNFPADFFNTTSKRGA
                     VFVTPGTGLRIDSTNFAAINAGLGDQFKFFSPKKTFMAVGSNQLDVQFQVVGTTANGL
                     VSGFGVVFSDVDRNGSTTIELFDANNVRIALLKAPNHSGAQLLSFTGAVFASPIIARA
                     RITSGDAALNGTIVDVSAGGTADLVVMDDFVYGEPQPLPQ"
BASE COUNT          391 a          819 c          800 g          385 t
ORIGIN      
        1 cggtcactat tctagacgga tcgcttatgc tcagctacca gtggcgtgcc ggcgatgcaa
       61 cgcacgaatt gcgcctggtg caggtgcccg ggacggccgg agagcccttc gcgttcggcg
      121 cgggatcgcg acagcgtccg atcgccattc gagacttcca catcatggcg acgcccgtca
      181 cccaggcgct gtggcagcac atcatgggat ccaatccggc gcgccggccg gacctgcggt
      241 gtccggtaga gaacgtgtca tggtacgatg tcaccggacc ggacggcttt gtggagcgga
      301 tcaatgcgag cgcggcgctg gcggcggtgt caggcgtcgc tccacaggcg cgtttccgtc
      361 tcccgtccga gacggagtgg gagtacgccg cgcgaggcgg cccgcactgg cgcgacggct
      421 tccggttcag cggcagcaac gacgccgccg ccgtcgcctg gtacgggcgc cgctggtccg
      481 gtgtccacga gctcatggtg cgtctgttcg ggagccgtcg cggctggcgg ctcgccaacc
      541 ggctgcgatt cggcatcccg aggccgacgc gcacccacga agtcgccacc aaggccccga
      601 accagctggg gatctacgac atgtcgggca acgtgtggga gtggtgccag gacgcctgca
      661 cggatgagct ggcggctgtt ccggaggacg gaacggcgtg gcccgggccg agtcctgagc
      721 ggcgtttgcg tggcggctgt caccacaact gggatctgca ctgcacggtg tactggcgct
      781 atggcatcga gccggacgcg cacgatggct gtatcggcct gcggttggcg ctctcctgac
      841 tcctccggcg aaggtccccg ggcttctctc acggttgttc cggccgccgc ttcgctccgc
      901 ggaagtacgc cccggcctcc ggacgcagca gcaggtacgc cactcccgcc agtaatgccg
      961 cgtggacggc tgtctcggac caggagtgga atgcgctcag gatgacgtgg tacgcgatcc
     1021 acgccagcac cagccagcgc gcccagtcag ccccgcgaag catgaacgct ccaccgatca
     1081 ccgccagcag ccggaggaag agcacccaca ccgcctcata gtcgagcggg cccgacgtct
     1141 cgagcgcggt cgcatggtag acgagtccga cgataccggc cgcgatgaac agccagccga
     1201 tcacggtgac cgatcgcggc cgtccgctcg gcaggggggc gttggtcatg gtgcgtcagg
     1261 gatccatggt gatcgtagtc cagcgaccga cacgtccctt ccgatgtcca ccacacgaca
     1321 gcgatgctgt gtctgattct gccggcgcgg cctgaccact agccttgtct ccgtcagcga
     1381 tggaaaccac tcacgacgga gaagacgatg aaaccgacaa tctatgcccc aatcctgggc
     1441 ctggtggctt tggcggcgtg cagtagcctc gataccccca cgcagcctca gggccccgcg
     1501 tacaccacct acgagaccct gggcgatagt ctctcgatcg cgaccaagct ggacgagttc
     1561 cgggccgcgc tgggtggcgt cctcaacgcc cccaacgcgc ctcccgccgc cagcggccgg
     1621 cgcgagatca actgggacgg cgcccccgcg gcgttgacca acgtcgacaa cttcccggcg
     1681 gacttcttca acaccacctc gaagcggggc gccgtattcg tgacccccgg cacgggtctg
     1741 cggatcgaca gcaccaactt cgccgcgatc aacgccggcc tgggtgacca gttcaagttc
     1801 ttttcgccca agaagacctt catggcggtg ggcagcaacc agctcgatgt ccagttccag
     1861 gtggtcggca ctacggccaa cgggctggtc agcggcttcg gcgtcgtctt ctccgacgtg
     1921 gaccggaacg gctccacgac catcgaattg ttcgacgcga acaacgtccg gatcgccctg
     1981 ctcaaggccc ccaatcacag cggcgcgcag ctgttgtcgt tcaccggcgc ggtcttcgct
     2041 tcgccgatca tcgcccgggc gcgcatcacc agtggcgacg ccgccctgaa cgggacgatc
     2101 gtcgatgtga gcgcgggcgg caccgcggac ctcgtcgtca tggacgattt cgtctacggc
     2161 gagccgcagc cgctgccgca gtgactcggt ccactcactc atcttgcacg gaggcagtca
     2221 tgcgatacac gaaatggatg acgggcgccg ccctgctcgc cctggcggcg tgcggcggcg
     2281 tgacttcacc cggcgggggc ggcgggggcg gtggcggtgg cggcggcggt ggcggcggcg
     2341 gcccggtcgg cgatatcacg gtggggaaca tcttcttccg gagtgtccac aacgg
//
LOCUS       DBNGZW010000072         3694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_6583, whole genome shotgun sequence.
ACCESSION   DBNGZW010000072 DBNGZW010000000
VERSION     DBNGZW010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_6583"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2602)
                     /locus_tag="AC16JC_01845"
     CDS             complement(<1..2602)
                     /locus_tag="AC16JC_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR5163470.1"
                     /translation="MTSRRSFLARTGVGGALLVVPPWLRLPLAFPRSPNAPPRDLSES
                     LVSGLEWRLLGPFRGGRVDAVSGVPGRPNEFYFGHVNGGVWKTIDAGRVWEPVFDAQP
                     VASIGALSVAPSAPDVIYVGSGESTLRDSTGYGNGMYKSTDAGRTWTHIGLDDTHHIG
                     KIAVHPTNPDIVFVAAIGHYYGPSTDRGVFRSKDGGRTWQKVLYKNANVGAIEVVIEP
                     GNPQVLYAGLWNTRRPPWFVYPPTNGPGGGVYKSSDGGDSWTQLTQGLPPEGIGRTGI
                     AVAPSRPERLYAVVDCLLPEPGAPPPPPPTPGGPPQRDTTPRQGGFFRSDDGGATWRR
                     RSSDTALWGRGWYFEHVVVDPRDPDLVYVSNVSVSRSSDGGQTWVPLRGSPGGDDYHQ
                     AWISPDDPNTMIVASDQGCIITRNAKADDPREVTWSSWLNQPTAQLYHLSVDYRFPYW
                     VTGAQQDSGAVAVRSRGKFGQISIRDWEPIGAGGESGCTAGDPLHPGIVFGGTGSRWD
                     LEQNRMIPGTTAPTPTNKEPVRRDWTQPLVVSPADPHALYYADQFLFKSTDAARTWRQ
                     ISPDLTRPAPGVPPTLDAAAAADTDQNGQRGVIYTIAPSPLRASLVWVGTDDGVIQLT
                     QDDGKTWQDVTPAAIGPWSRVTTLEASHFDARTAYASVDRHQLEDFAPYIYRTRDLGR
                     TWQRISTGLQAGVYVHAVKEDPARKGLLFAGTERGVFLSLDDGDHWQPLQRNLPVTSM
                     RDLQVYGNDLIVATHGRGFWVIDDISPLRQIDAAVAADAWLFKPADAVLVDQGDDNGT
                     PFQKDEPQAENPADGAYLYYYLKTDAPGPVTLEILDAKGTSLHTFSSEAPPARPARPG
                     IPNTTALWRPE"
     gene            complement(2614..3567)
                     /locus_tag="AC16JC_01850"
     CDS             complement(2614..3567)
                     /locus_tag="AC16JC_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014020792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-disaccharide hydrolase"
                     /protein_id="HIR5163471.1"
                     /translation="MTTTERILCTLLGLGIGVRDLAAQQPVEPPVTGRWDITVAMPPG
                     GYPSWLEVQWSGDRTLVGRFVGRFGSSRPISRLEFTHDTLRFSIPPQWEQGDADLRFE
                     AALADDRLSGWMTDANGERRNWSARRAPTLRRATAPAWGRPDTLFSGANIARWTPSGA
                     NSHWQVVDRVLTNTAAGANLVTRDSFTDFTLHLEFRYPAGGNSGIYLRGRYEVQVEDT
                     PGAEPKNDGLGSIYGFLLPSENAAKQPGEWQSYDITLVGRLVTVVLNGRRIICEQPIP
                     GITGGALDSHEEAPGPIYLQGDHGPIEYRNIVLTPASRRRP"
BASE COUNT          505 a         1225 c         1374 g          590 t
ORIGIN      
        1 gctcgggccg ccacaatgcg gtcgtgttgg ggatcccggg ccgcgccggc cgcgcgggcg
       61 gggcttcgct cgagaacgtg tgcaggctcg tccccttggc gtcgaggatc tcgagcgtca
      121 ccgggcccgg cgcgtcggtc ttcaggtaat agtagaggta ggcgccgtcc gccgggttct
      181 ccgcctgtgg ctcgtccttc tggaacgggg tgccgttgtc gtcgccctga tcgacgagca
      241 cggcatcggc cggcttgaag agccaggcgt cggccgccac cgcggcgtcg atctgtcgca
      301 gcgggctgat gtcgtcgatg acccagaacc cgcggccgtg cgtcgccacg atcaggtcat
      361 tgccgtacac ctggagatcc cgcatcgacg taacggggag gttgcgctgc agcggctgcc
      421 agtgatcgcc gtcgtccagc gacaggaaca cgccgcgctc ggtgccggca aagagcagtc
      481 cctttcgggc cggatcttcc ttcaccgcgt ggacatagac gccggcctgc aggccggtgc
      541 tgatgcgctg ccaggtcctg cccaggtcgc gggtgcgata aatgtagggc gcgaagtcct
      601 cgagctggtg gcggtccacg ctcgcgtacg ccgtgcgcgc atcgaaatgc gaggcctcga
      661 gcgtcgtgac ccggctccac gggccgatcg cggcgggggt cacgtcctgc caggtcttgc
      721 cgtcgtcctg cgtgagctgg atgaccccgt cgtcggtgcc gacccatacc agcgaagcgc
      781 ggagtggcga cggcgcgatc gtgtagatca cgccgcgctg gccgttctgg tcggtatcgg
      841 cggccgcggc cgcgtcgagg gtgggtggga cgccgggagc ggggcgcgtc aggtccgggc
      901 tgatctgcct ccacgtgcgg gcggcgtcgg tcgacttgaa caggaactgg tcggcgtagt
      961 acagcgcgtg cgggtcggcc ggcgacacga cgagcggctg cgtccagtcg cgccgcaccg
     1021 gctccttgtt ggtcggtgtg ggcgccgtgg tgcccgggat catgcggttc tgctccaggt
     1081 cccagcggct gcccgttccc ccgaagacga tgccggggtg cagcggatcg cccgccgtac
     1141 agccgctctc gcctccggcg ccgatcggct cccagtcgcg tatcgagatc tgcccgaact
     1201 tgccgcgcga gcgcacggcc acggcgccgc tgtcctgctg cgcgccggtc acccagtagg
     1261 ggaagcggta gtccaccgag aggtggtaca gctgggccgt gggctggttg agccacgagc
     1321 tccaggtgac ctcgcgcgga tcatcggcct tcgcgttgcg ggtgatgatg catccctgat
     1381 cgctcgccac gatcatcgta ttcggatcgt cgggggagat ccacgcctgg tgatagtcgt
     1441 cgccgccggg tgagccgcgc agcgggaccc acgtctggcc gccgtcgctc gagcgcgaga
     1501 cggagacgtt ggagacgtac acgaggtcgg gatcgcgcgg gtcgacgacg acgtgctcga
     1561 agtaccaccc gcgtccccac agcgcggtat cggacgaccg gcgcctccat gtggcgccgc
     1621 cgtcgtcgga gcgaaagaac ccgccctggc gcggcgtggt gtcgcgctgc ggcgggccgc
     1681 ccggcgtggg cgggggcggt ggtggggcgc cgggttcggg gagcaggcag tccacgacgg
     1741 cgtacaggcg ctcgggccgg ctcggtgcca cggcgatgcc ggtgcgcccg atcccctccg
     1801 gcggcaggcc ctgggtcagc tgcgtccagg agtcgccacc gtcgctggac ttgtagacgc
     1861 cccctccggg cccgttcgtc ggcgggtaga cgaaccaggg cgggcggcgc gtgttccaca
     1921 ggcccgcgta cagcacctgc gggttgcccg gttcgatcac gacctcgatc gcgccgacgt
     1981 tcgcgttctt gtacagcacc ttctgccagg tccggccccc gtcctttgag cggaacacgc
     2041 cgcggtcggt gctcggcccg tagtagtggc cgatggcggc gacgaagacg atgtcggggt
     2101 tcgtcggatg gacggcgatc ttcccgatgt ggtgcgtgtc gtcgagaccg atgtgagtcc
     2161 aggtccggcc ggcgtcggtc gacttgtaca tgccgttgcc gtagccggtc gaatcgcgca
     2221 gcgtggactc gccgctcccc acgtagatca cgtccggcgc ggagggggcc acgctgagcg
     2281 cgccgatcga agcgacgggc tgtgcgtcga agaccggctc ccacacgcgg cccgcgtcga
     2341 tcgtcttcca cacgccgccg ttcacgtggc cgaagtagaa ctcgttgggc cggccgggca
     2401 cgccgctcac cgcgtcgact cggccgccgc ggaacggacc gagcaggcgc cactcgaggc
     2461 ccgagaccag actctcggac aggtcacggg gaggcgcgtt gggggatcgg gggaaggcga
     2521 gcggcaggcg cagccagggg ggaaccacga ggagggcacc gccgacgccg gtgcgggcca
     2581 gaaagcttcg tcgcgatgtc atgtgtggaa ggctcaggga cggcggcggc tggccggcgt
     2641 cagcacgatg ttgcggtact cgatgggccc gtgatctccc tggaggtaga tcgggcccgg
     2701 cgcctcctcg tggctgtcca gggcgccccc ggtgatcccg gggataggtt gctcgcagat
     2761 gatgcggcgt ccgttcaaga ccaccgtcac cagacgtccg acgagcgtga tgtcgtagga
     2821 ctgccactcg cccggctgct tcgcggcatt ctcgctgggc aggagaaatc cgtagatgga
     2881 cccgagaccg tcgttcttgg gctccgcgcc gggcgtatcc tcgacttgca cttcgtatcg
     2941 cccgcgcaga tagatcccgc tgttgccgcc cgcggggtag cggaattcga gatgcagcgt
     3001 gaagtccgtg aagctgtcgc gggtcacgag gttggcgccg gcggcggtat tggtgagcac
     3061 gcgatccacc acttgccaat ggctgttggc tccgctgggc gtccagcggg cgatgtttgc
     3121 gccgctgaag agcgtgtcgg gtcggcccca ggccggcgcc gtggcccggc gcagggtcgg
     3181 cgcccggcgg gcgctccagt tgcgccgctc gccgttggcg tcggtcatcc accccgaaag
     3241 cctgtcgtcc gccagcgcgg cctcgaagcg caggtccgcg tcgccctgct cccattgggg
     3301 cggaatcgag aatcgcagcg tgtcgtgcgt gaactcgagc cgggagatcg gccgcgagct
     3361 gccgaagcgg cccacgaagc ggcccacgag cgtccggtct ccggaccact gcacctcgag
     3421 ccaggaaggg tagcccccgg gcggcatcgc gacggtgatg tcccagcgcc cggtgacggg
     3481 gggctcgacg ggctgctgcg cggccaggtc gcgcacaccg atgccaagcc cgaggagggt
     3541 acacaggatc cgctcggtcg tcgtcatcgg tgacctctcg tgtcggggag cgctaggcgt
     3601 gtgaagccca ggcggtctcg ccgggcgtgt acggcagaca gggatcgaag cgcccgggcg
     3661 tttcctcgta acgcatcgcc tgggtgcagc cgat
//
LOCUS       DBNGZW010000073         5191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1642546, whole genome shotgun sequence.
ACCESSION   DBNGZW010000073 DBNGZW010000000
VERSION     DBNGZW010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5191
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1642546"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..240
                     /locus_tag="AC16JC_01855"
     CDS             <1..240
                     /locus_tag="AC16JC_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020013302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HIR5163472.1"
                     /translation="VNEPQVLLADEPTGNLDERATRGVFQLLRDINASGTAVVMATHD
                     LELVRSAPYRVIELQDGAVVYDSAVDEAAAGGEPA"
     gene            237..1094
                     /locus_tag="AC16JC_01860"
     CDS             237..1094
                     /locus_tag="AC16JC_01860"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR5163473.1"
                     /translation="MTLTVREALLAFRRAPLLSALSVTTIAFSLFVLGLFGLVAVNLR
                     QALRGVAERVEIVAYVLPGTQIETVTLAEQDIEAFPEVLSADYVPEDSALARARRELV
                     EFRDLLREMERNPLPSSIEVRLKPGYQDAAHVNAVAERLRSFNFVDDVRYGRDWVEKL
                     DRLRGLAAAVGVVVGAAFAVVAVIIIGTTIRMAVLQRSREIAIMRLVGATDGFIRRPF
                     LLQGAIKGLLGGLVAVGLSYAAYVLINRFLIASSFFTALQALAVVAFGTVIGLMGSLL
                     SVGRHLRRV"
     gene            1166..2335
                     /locus_tag="AC16JC_01865"
     CDS             1166..2335
                     /locus_tag="AC16JC_01865"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR5163474.1"
                     /translation="MTVLLDVAALSAPLLAQRPTLEQQMRDNQQRLEGIRRERNQAQQ
                     DLERLRAQVHSLADELTNLEHQKETTNRIVNELDRQILQLGGAIDRITVDLVLAQDAL
                     DEKRAVAERRLVDIYKRGPLYAYQVLFAAESFGDLLSRYKYLYLISRQDRQLANEMNR
                     LRDRIARERRGLVEARDALERRRSERSEELQHFVDLATQRQRRLRETRRDVERVQERL
                     TATDAEERRVANAIAALERARREAEARGAVPTTGAITSGSLGSLDWPVTGRVVYDFGP
                     AAGPDNTRITWHGIGIAADEGTAVKAVAPGTVALVGPLGTYLTTVIVDHGGGFYTIYA
                     SLNDAAVSKGDRVIRGQLLGHVGGGTTEQGPHLHFEIRGPGQIALDPLNWLKPKP"
     gene            2336..3277
                     /locus_tag="AC16JC_01870"
     CDS             2336..3277
                     /locus_tag="AC16JC_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR5163475.1"
                     /translation="MRQAETSSENVERERPARTYSLDGSARRCRSTLLLDTVLLLCAC
                     AAPTNRLEVAGTVEIRDINVAPLAAGRVVRLLKDEGDAVRRGDTIAVLEQPGLPALIG
                     QRQAQAQAAQRRTAEVRAAVADSTRAANDLSRAGPLREQGIVSAQYFDALTTAAAAAT
                     ARLQAVRAAASESRAAAAGLAATEAIRDELVLLAPADGVILTRYLEPGEAAGVGVPVV
                     SLGVVAQPWIRAYVGEPYIARVHLGQTVTIRADGLAAPVSGRIVDIATRAEFTPRAAL
                     TDRERADLVFAIKVGFDDPAGRLKAGLPVTLELPLTP"
     gene            3274..4008
                     /locus_tag="AC16JC_01875"
     CDS             3274..4008
                     /locus_tag="AC16JC_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005836383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5163476.1"
                     /translation="MSEVAVDIQQLTRRFGALTAVSGLTLQIPRGQVFGLLGPNGSGK
                     TTTIRMLCGLLDPSEGTASVAGIDIRTAPERVKMQIGYMSQRFGLYEDLTVAENLDFY
                     GGIYGLTGRARTTRTAEVVGFLGIGGRLEQLAGQLSGGWKQRLALGCALLHQPPVLFL
                     DEPTAGVDPAARRNFWQAIHDLARGGTTVLVTTHYMDEAERCDRLAMMSLGHLIALGT
                     PAEVAAQVGGRTLEDAFIILQERDER"
     gene            4005..5117
                     /locus_tag="AC16JC_01880"
     CDS             4005..5117
                     /locus_tag="AC16JC_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004099039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5163477.1"
                     /translation="MMSRFWPMFRKEFIQMKRDRLTLGIMIGIPVVQLLMFGYAIQTD
                     VRHIPTVVLDRSRTPESRDLIAAFENTGSFRIVDHVDGQPALDAAIERGRAQAGIIVP
                     PEYARDLGRGRTATVQVVIDASDPLSSQSALAAAAGVGQVRNLGALDAAAGRASIPID
                     VRVRPRYNPGLRSPNYIVPGLVGVILTLTLVLITAMAIVRERERGTLEQLIVTPITKT
                     ELMLGKIAPYIGVGIVQMTAVLTLGRFVFDVPLSGSLWLLYGVSLIFVIASLSFGLFI
                     STMVKNQQQAMQVSFFFVMPNILLSGFMFPRAAMPALFQWFSLALPLTHYLQVLRGIL
                     LKGVGLDAIWPQVLILCGFAVALISLAVRRFHKTLD"
BASE COUNT          767 a         1807 c         1807 g          810 t
ORIGIN      
        1 gtgaacgagc cgcaggtcct cctggcggac gagcccaccg gcaatctcga cgagcgcgcc
       61 acgcggggcg tgttccagct gctgcgcgac atcaatgcca gcggaacggc ggtggtcatg
      121 gcgacccacg atctggagct ggtgcgctcg gccccctacc gggtgatcga gctccaggat
      181 ggcgccgtgg tctacgactc cgccgtggac gaggcggcgg ccggaggcga acccgcgtga
      241 ccctgaccgt gcgcgaggcc ctgctcgcgt tccggcgcgc gccgctcctg tcggcgctct
      301 cggtcaccac gatcgcgttc tcgctgttcg tgctcgggct gttcgggctc gtcgccgtga
      361 acctccggca ggcgctgcgc ggcgtcgcgg agcgggtcga gatcgtcgcg tacgtcctcc
      421 ccggtacgca gatcgagacg gtcaccctgg ccgaacagga catcgaggcc tttcccgagg
      481 tgctgtccgc cgactacgtg cccgaggaca gcgcgctggc gcgcgcgcgc cgcgagctgg
      541 tggaattccg cgacctgttg cgcgagatgg agcgcaatcc gctcccctcc tccatcgagg
      601 tcaggctcaa gccggggtac caggacgcgg cccacgtgaa cgccgtcgcc gagcggctgc
      661 ggagcttcaa cttcgtggac gacgtgcgct acggtcgcga ctgggtggaa aagctggacc
      721 ggctgcgcgg gctcgccgcc gcggtcggcg tggtggtcgg cgcggcgttc gccgtggtcg
      781 ccgtgatcat catcggcacc accatccgca tggccgtgct gcagcgcagc cgcgagatcg
      841 cgatcatgcg gctcgtgggc gcgaccgacg ggttcatccg gcgcccgttc ctgcttcagg
      901 gcgcgattaa ggggctgctc ggcgggctag tcgccgtcgg gctgtcctac gccgcctatg
      961 tgctcatcaa ccggttcctg atcgcctctt ccttcttcac ggcgctgcag gcgctggccg
     1021 tcgtggcgtt cgggacggtc atcgggctga tggggagtct cctcagcgtc gggcgacatt
     1081 tgagaagagt atgagacgtc aagcgaaggc gtccagcaac ggcgtcgagc ggaggcgtgg
     1141 atacggtctt gctcgacgca tttgcttgac ggttctgttg gacgtagctg cattgtcggc
     1201 gccgctgctg gcccagcgcc ccaccctcga gcagcagatg cgcgacaacc agcagcgcct
     1261 ggagggcatc cgccgcgagc ggaaccaggc gcagcaggat ctggagcggc tgcgcgccca
     1321 ggtccacagc ctcgccgacg agctgacgaa cctcgagcat caaaaagaga cgacgaaccg
     1381 catcgtgaac gagctggacc ggcaaatcct ccagctgggc ggcgcgatcg accgcatcac
     1441 ggtggacctg gtgctggcgc aggacgcgct ggacgagaag cgcgccgtgg cggagcggcg
     1501 gctcgtcgac atctacaagc gcggcccgct gtacgcctac caggtgctgt tcgcagccga
     1561 gagcttcggc gatctcctgt cccggtacaa gtacctctac ctgatcagcc gccaggaccg
     1621 ccagctcgcc aacgagatga accggttgcg ggatcgcatc gcgcgggagc ggcgcggcct
     1681 ggtggaagcc cgcgacgccc tggagcgtcg ccgcagcgag cggtccgagg agctgcaaca
     1741 cttcgtcgat ctcgccacgc agcggcaacg ccggttgcgc gagacccgcc gcgacgtgga
     1801 gcgggtgcaa gagcggctca ccgccaccga cgccgaggaa cggcgtgtcg ccaacgccat
     1861 tgcggcgctc gagcgcgcgc gccgcgaggc cgaggcccgg ggcgccgtgc cgacgaccgg
     1921 cgcgatcacg agcggaagtc tgggctcgct cgactggccg gtgaccggcc gcgtggtgta
     1981 cgatttcggc cccgccgcgg ggcccgacaa cacgcgcatc acctggcatg gcatcggcat
     2041 cgccgcggac gagggcacgg ccgtgaaggc ggtcgcgccc gggacagtcg ccctggtcgg
     2101 gccactcggg acctacctca ccacggtgat cgtggatcac ggcgggggct tctatacgat
     2161 ctacgcctcg ctcaacgacg cggcggtctc caagggcgac cgcgtgatcc gggggcagct
     2221 gctcggtcac gtcggcggcg gcacgaccga gcagggaccg cacctgcact tcgagatccg
     2281 gggtccgggg cagatcgcgc tcgatccgct caactggctg aagccgaagc cctagatgcg
     2341 tcaagcagag acgtcgagcg agaacgtcga gcgagaacgt ccagcgcgga cctattcgct
     2401 cgacggctct gctcgacgat gtcgctcgac gctgttgctc gacactgtac ttctgttgtg
     2461 cgcctgtgcc gcccccacga accgcctgga ggtggccggc accgtcgaaa tccgcgacat
     2521 caacgtggct cccctcgctg ccggacgggt cgtacgcctc ctcaaggatg agggtgatgc
     2581 ggtgcggcgc ggcgacacca tcgcggtgct cgagcaaccc ggcctcccgg cgctcatcgg
     2641 ccagcggcaa gcccaggcgc aggccgctca gcggcgcacg gccgaggtcc gtgccgcggt
     2701 ggccgacagc acgcgagcgg cgaacgacct ctcccgcgcg ggcccgctgc gcgagcaggg
     2761 catcgtgtcg gcgcagtact tcgacgccct gacgaccgcc gccgccgcgg ccaccgctcg
     2821 gctgcaggcc gtgcgggcgg ccgcgagcga gtcgcgcgcc gccgccgccg gcctcgcggc
     2881 caccgaggcg atccgtgacg agctcgtgct gctcgcgccg gccgacggcg tcatcctcac
     2941 gcgctacctc gagcccggcg aggccgcggg tgtcggcgtg ccggtcgtgt cgctcggcgt
     3001 cgtggcgcaa ccctggatcc gcgcgtacgt cggcgagcca tacatcgcgc gggtgcatct
     3061 cggccagacg gtgacgattc gggccgacgg tctggcggcg cccgtcagcg gacgcatcgt
     3121 cgatatcgcg acgcgcgccg agttcacgcc gcgcgccgcc ctcaccgacc gcgagcgcgc
     3181 cgatctcgtg ttcgccatca aggtgggctt cgacgatccg gccggccgtc tcaaggcggg
     3241 cctgcccgtg acgctcgagc tgccgctcac gccgtgagcg aggtcgcagt cgacatccag
     3301 cagctgacgc gccgcttcgg cgctctcacc gccgtttccg gactgacgct ccagatcccc
     3361 cgcgggcaag tgttcggcct gcttggaccc aacggctcgg gcaagaccac gacgatccgc
     3421 atgctgtgcg ggctgctcga tccctccgaa gggaccgcct ccgttgcggg aatcgacatc
     3481 cgcacggcgc ccgagcgcgt gaagatgcag atcggctaca tgtcccagcg gttcgggctg
     3541 tacgaggatc tcaccgtcgc cgagaacctc gacttctacg gcgggatcta cggcctgacc
     3601 ggccgggcgc gcaccacgcg cacggcggag gtggtggggt tcctgggcat cggcggacga
     3661 ctcgagcagc tggcggggca gctgtcgggc ggctggaagc agcgcctggc cctgggctgc
     3721 gcgctgctgc accagcctcc cgtcctgttc ctcgacgagc cgacggccgg cgtggacccg
     3781 gccgcgcgac gcaacttctg gcaggccatc catgatctcg cgcgcggcgg caccaccgtc
     3841 ctggtgacga cgcactacat ggacgaggcc gaacgctgcg accggctcgc gatgatgagc
     3901 ctggggcacc tgatcgccct gggcacgccg gcggaggtcg cggcgcaggt cggcggccga
     3961 acgctcgagg acgccttcat catcctccag gagcgcgacg agcgatgatg tcccgcttct
     4021 ggccgatgtt ccgcaaggaa ttcatccaga tgaagcgcga ccgactcacg ctgggaatca
     4081 tgatcggcat cccggtggtg cagctgttga tgttcgggta cgcaatccag accgacgtac
     4141 ggcacatccc gaccgtggtg ctggaccgct cgcgcacccc cgagagtcgc gacctgatcg
     4201 cggcgttcga gaacaccggg agcttccgca tcgtggacca cgtggacggc cagccggcgc
     4261 tcgacgcggc gatcgaacgg gggcgggcgc aggccggcat catcgtcccc ccggagtacg
     4321 cccgcgatct cggccgcggc cgcaccgcga cggtgcaggt cgtgatcgac gcctccgacc
     4381 cgttgtcgtc acagtccgcg ctcgcagcgg cggcgggcgt cggccaggtg cgcaatctgg
     4441 gcgcgctcga cgccgccgcc ggccgcgcga gcattcctat cgacgtgcgc gtgcgaccgc
     4501 gctacaaccc cggcctgcgc agcccgaact acatcgtgcc cgggctggtg ggggtgatcc
     4561 tcacgctcac gctcgtgctc atcacggcga tggcgatcgt ccgggagcgc gagcgcggca
     4621 ccctggagca gctcatcgtc acgccgatca ccaagaccga gctgatgctg gggaagatcg
     4681 ccccttacat cggcgtgggc atcgtgcaga tgaccgcggt gctcacactg ggccgcttcg
     4741 tgttcgacgt gccgctctcg ggcagcctgt ggctgctcta tggcgtgtcg ctcatcttcg
     4801 tgatcgccag cctctccttc ggcctgttca tctccaccat ggtcaagaac cagcagcagg
     4861 ccatgcaggt cagcttcttc ttcgtgatgc cgaacatcct gctctccggt ttcatgtttc
     4921 cgcgcgcggc gatgccggcg ttgttccagt ggttcagcct ggcgctgcca ctcacgcact
     4981 accttcaggt gctgcgcggc attctgctca agggcgtggg cctggacgcg atctggccgc
     5041 aggtgctgat cctgtgtggt ttcgccgtgg cgttgatctc cctggccgta cggcgattcc
     5101 acaagacgct ggactgaacg aatcggggag tgggagtggg gagtggtacc tgacggtggg
     5161 gcatgggact gcgtctgctt ccgtcgagta c
//
LOCUS       DBNGZW010000074         6955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1302189, whole genome shotgun sequence.
ACCESSION   DBNGZW010000074 DBNGZW010000000
VERSION     DBNGZW010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1302189"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1225
                     /locus_tag="AC16JC_01885"
     CDS             <1..1225
                     /locus_tag="AC16JC_01885"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004766 - spermidine synthase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="spermidine synthase"
                     /protein_id="HIR5163478.1"
                     /translation="SSEQFDVIVSEPSNLWVAGVATLYTPEFFRIVRRRLSEDGVFCQ
                     WVQLYQLPVSVVAGIVRNLREVFPHVAVTSAGSYDLLVLGSARPLAPDTAWVTALLGG
                     RSTLGDAARGYLLLAKPVDYFDRQVLGEAGVARLVAGASLIHTDDRPQLEFVAARRFL
                     DGGSTGSILDSLAAIERPVENKDGLTSLRLARAMSARLGDAAGTGFLLAARSAQPDNL
                     EWRVALAAIGVSQGDSALADSVYPRLLERREDPRVLLLAGLLATARGQAVPARALFAR
                     TLTAGGDSARVYAALAQLDARDSLWTAAMQHVRASLHDVRNTFRSAFPRDLFSPTLAD
                     LAGRGPPAAVDSLLAEMVSVRPGWARLYELRAVVALRDGACDAAGEQFLVLRQFGIQR
                     GDDRQLIQQCRRGRI"
     gene            complement(1233..3074)
                     /locus_tag="AC16JC_01890"
     CDS             complement(1233..3074)
                     /locus_tag="AC16JC_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5163479.1"
                     /translation="MTDHDRSLTGRLREALGSSYTVENEIGRGGMGVVFQARDERLKR
                     QVAIKVLPPELAFREEVRLRFLREAETAARLTHPHIVPIHSVGEGPDGLVYFVMAFID
                     GEPLSARLKRRGRLPAEEGRRILMETADALGAAHSLGIIHRDVKPDNIMLEGSRGRVM
                     VTDFGIAKALSSSTGGGLTGTGVAIGTPHYMSPEQAAGDREIDGRSDLYSLGVVGYQM
                     LAGELPFQAPTVPGILIKQITEAAPYIADRVPEMPEDLASCVMRCLEKEPESRWPTAE
                     ALRRALESRTAAPYRPRRSAPGVSRRSPSGLRPMPPVASPAIPRSPSIPRRGRAPAKA
                     VEAPGGEPAIVSQTRGSFVSWASVSAGLLVLDLMNGGGLNWSYWVIGLWGAFGVLPRF
                     TKLWQAGYSWRDVLHRPLAADAADVRLGLAAPGKPGALAAPTTGEYGRAAEQIRQVQG
                     DYQAIMRVVARLPKSEQKLLPDVSATANALLKRAEELARTLHTLSAGVESGALDRLDA
                     RLADLRQQPDSTEQERQVGLLQRQHQALSDLLKRRQIIEQQLESCVLAMQTMRFDLLR
                     LRSAGVAAVLGDLTQATQQARALSRDVDHAIEAAGEIRQMLGESPPP"
     gene            3334..5799
                     /gene="lon"
                     /locus_tag="AC16JC_01895"
     CDS             3334..5799
                     /gene="lon"
                     /locus_tag="AC16JC_01895"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR5163480.1"
                     /translation="MTDRLTLPVLPLREVVLFPGVTTVIGVGRPGTLRAVEAASKTDS
                     RLVFAVSQRENVDHVTPAALYTMGTVARIAQLQRGLNGVQLLLQGERRSTAVHYTEGE
                     GYLSAVVRDAPDLPPLDPQDPAFVALYREVRERAAELGQKSGLPEEVVKQVLEGVTDP
                     GRLADLVAGHIELKPAERQSLLESLAVEERLRRVLVAVQRQIGVLDAQEDIKSQVQEE
                     LGERQREMFLREQMKAIRKELGEEDEDGEVEELRKRLEALELPEAARKEVERELSRLE
                     RTSRDSMEAQVIRTYLETVAELPWNTRAAESLDLKRAAAILEEDHYGLQDVKDQVLEF
                     LAVRQLRQRQGTGVESQTEEKKTEERNKGEPKADAESKGSILLFVGPPGVGKTSIAKS
                     IARAMGRPYVRISLGGARDEADIRGHRRTYVGAMPGRIIQGMKQAGAKNPVFLLDEVD
                     KLGISYQGDPAAALLEVLDPAQNDSFTDHYLGVPFDLSEVLFIATANFPQNIPGPLLD
                     RLETVDFAGYTEREKLEIAKRYLVPRQLTENGLAVEQLAITDDGLGEIVSSYTREAGV
                     RQLEREISKLGRKVARRIADGNAEKVVVDRKDVHDLLGRPKVHPEHKAGEDQVGVATG
                     MYYTPVGGDIMFVEASLMRGKGDLVLTGQLGDVMKESARAALTYAKSHADRLGIPEVG
                     LADTDIHIHVPAGAIPKDGPSAGVTMATALVSALSRRPARHDLAMTGEITLRGRVLPI
                     GGVKEKVLGAVRAGIRTIVLPKENEPDLEDLPEEVRQSLEVHAVEELGEVLSLALRGA
                     KFEAGQLVFDGPAPVEPRMVSKS"
     gene            complement(5811..6410)
                     /locus_tag="AC16JC_01900"
     CDS             complement(5811..6410)
                     /locus_tag="AC16JC_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5163481.1"
                     /translation="MPRKLSNDRALRIGVAAAEEFAKRGFAGARVERIAAAARVNKQL
                     VFYYFHSKRGLFQTVLRRGVNDLEAALATISGEGRTPLERLREVLAALFEFLERHPQL
                     VALLSHAERLDVAPFAAPIRRLVVLLAEGQGLGLVRDDLDPHVAAAQALVLMLGYLRL
                     EPIIAASAPPLATDEPALRERWRRSAVDLVVNGVRAPAA"
     gene            complement(6473..>6955)
                     /locus_tag="AC16JC_01905"
     CDS             complement(6473..>6955)
                     /locus_tag="AC16JC_01905"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5163482.1"
                     /translation="AAGLAVRWPFMLRVAHWPIVGALFDRLRGRAATAGILKALYAVP
                     SRVTAADIDQYYAPLAEPGFGRCLRGVLGQFRFDALKDRLGQVATPTLVVWGTADRLI
                     PAAVGQSMADQIPGALLVRFPDAGHAVPEESPQAFNQALIGFLEHGLSTPPANIAAAR
                     "
BASE COUNT         1113 a         2319 c         2406 g         1117 t
ORIGIN      
        1 ctcatccgag cagttcgacg tgatcgtatc cgagcccagc aatctgtggg tggccggggt
       61 cgcgaccctc tacactccgg aattcttccg catcgtccgt cgccggctca gcgaagacgg
      121 tgtcttctgc caatgggtgc agctgtacca actaccggtc tcggttgtcg ccggcatcgt
      181 gcgcaacctc cgggaggtgt tcccgcacgt cgcggtcacg tcggcgggct cctacgacct
      241 cctggtgctc ggctccgcga gaccgctcgc gcccgatacg gcttgggtga ccgccctgct
      301 cggcggccgg agtacgctcg gggacgcagc ccgcggctac ctcctgctcg caaagccggt
      361 cgactacttc gatcgccagg tgctcggtga ggcgggggtc gcgcgtctcg tcgccggcgc
      421 ctccctcatt cataccgacg accgcccgca gctcgagttc gtggccgcgc ggcggttcct
      481 ggacggcgga tcgacgggga gcatcctcga ttcgctggcc gcgatcgaac gtccggtcga
      541 gaacaaggac ggcctgacgt cgctgcgact ggcgcgggcc atgtccgcgc gcctcggcga
      601 tgccgccggc acggggttcc tgctcgccgc gcgctctgca cagcccgaca atctcgagtg
      661 gagggtcgcc ctggccgcca tcggtgtgtc ccagggcgat tcggcactcg cggattccgt
      721 ctatccccgg ctgctcgagc gacgggagga tccacgtgtc ctgctgttgg ctggactgct
      781 cgcgaccgcc cgcgggcagg ccgtaccggc gcgtgccttg ttcgcgcgca cactgaccgc
      841 cggcggcgac agcgcccgcg tgtacgcggc cctcgcgcag ctcgatgccc gcgacagtct
      901 ctggaccgcc gccatgcagc atgtgcgggc gtcgctgcac gacgtccgga acacgttccg
      961 gtcggcgttt ccgcgggacc tcttctctcc cacgctcgcg gatctagccg gccggggtcc
     1021 gcccgcggcc gtggacagtc tgctcgcgga gatggtctcg gttcgtccgg ggtgggccag
     1081 actctacgag ctgcgggcgg tggtggcgct gcgggacggt gcatgcgatg ccgctggaga
     1141 gcagttcctc gtcctgaggc aattcggcat tcagcggggt gacgatcggc agctgatcca
     1201 gcaatgccgc cgaggccgga tctaggcagt cctcaagggg gaggactctc gcccagcatt
     1261 tgtcggatct ctccggcggc ttcgatggcg tgatcgacgt cgcgtgacag cgcgcgcgct
     1321 tgctgcgtcg cttgcgtgag atccccgagg accgccgcca caccggccga ccgcagccgg
     1381 agcaggtcga agcgcatcgt ctgcatcgcg agtacgcacg actcgagctg ctgctcgatg
     1441 atctggcgtc gcttcagcag gtcgctcaat gcctggtgct gtcgctggag caatccgact
     1501 tgccgttcct gttcggtgga atccggctgt tgccgcaagt cggccagacg tgcgtcgagc
     1561 cgatcgagcg caccggactc cactccggcg ctcagcgtgt gcagcgtccg ggccagctcc
     1621 tccgcgcgct tgagcagcgc gttggcggtc gcggaaacgt cggggagcag cttctgctcg
     1681 gatttcggca ggcgcgccac cacccgcatg atcgcctggt agtcgccctg cacctgccgg
     1741 atctgctcgg ccgcgcggcc gtactcaccc gtggtcgggg cggccagcgc gcccggcttc
     1801 ccgggggccg ccaatccgag ccgcacgtcc gcggcgtcgg cggccagcgg ccggtgcagc
     1861 acgtcgcgcc aggagtagcc tgcctgccag agtttggtga accgcggcag cacgccgaac
     1921 gccccccaga ggccgatcac ccagtacgac cagttcagcc cgccgccgtt catcaggtcg
     1981 agcaccaaca aacccgcgct gacggaagcc caggacacga agctcccacg cgtctggctg
     2041 acgattgccg gctcgccccc cggcgcctcg accgctttgg cgggagcccg gccacggcgg
     2101 ggaatcgagg gactgcgcgg tatggcgggt gaagcgaccg gcggcatcgg tcgcagcccg
     2161 cttggacttc ggcgcgaaac gccgggtgcc gaacgccgcg gccgataggg cgcggcggtc
     2221 cgcgactcca gcgcgcggcg cagcgcctcc gcggtcggcc accggctttc cggctccttt
     2281 tcgaggcagc gcatcacgca cgacgccaga tcttcgggca tctccggcac gcggtcggcg
     2341 atgtaaggcg ccgcttcggt gatctgtttg atgagaatgc cggggaccgt cggtgcctgg
     2401 aacggcaact ctcccgccag catctggtag cccacgacgc cgaggctgta caggtcggag
     2461 cggccgtcga tctcacggtc tcccgctgct tgctcgggac tcatgtagtg cggcgtgccg
     2521 atggccacac ccgttcccgt cagccccccg cccgtggacg aggagagtgc cttggcgatg
     2581 ccgaaatcgg ttaccatgac ccggcctcga ctgccctcga gcatgatgtt gtcgggcttg
     2641 acgtcccggt ggatgatgcc gagcgaatgg gcggcgccga gcgcgtcggc tgtctccatg
     2701 agaatgcggc gcccctcttc ggcaggcagc cggccccggc gtttgaggcg cgccgagagt
     2761 ggctcgccgt cgatgaacgc catgacgaaa tagaccagcc cgtccggccc ctcgccgaca
     2821 ctgtgaatcg ggacgatatg gggatgcgtc agccgggcgg ccgtctccgc ttcgcgcagg
     2881 aaacgcagcc gcacctcttc ccgaaacgcg agctcgggcg gcaggacctt gatcgccacc
     2941 tgccgcttga ggcgctcgtc cctggcctgg aacaccacgc ccatgccacc gcggccgatc
     3001 tcgttctcga ccgtgtacga actccccagc gcctcccgaa gccgtccggt aagcgatcgg
     3061 tcgtgatcgg tcatctcggg tcgaatcgca tggcggacct agattagccc tctccgaagt
     3121 gccgtgcaac ctttcaacat actgagactt agcggatttc ggatgccatc ctgacggtcg
     3181 ccgcgggtct tcgacgagcg gatttggcac gccgtgccca tttggcggtc ggacgacgga
     3241 acgttccctc agggccgggt gttctcgctt ggcatggccg atgcgttgcg cgatcggtac
     3301 catggaccta tggcatctgg agcgatgaca cacatgactg atcgtttgac gttgccggtt
     3361 ctgccgctgc gggaagtggt gttgttccct ggcgtgacga ccgtgatcgg cgtcggccgc
     3421 ccggggacgc tacgtgccgt cgaggccgcg tccaagaccg acagccggct ggtgttcgcc
     3481 gtctcgcagc gggagaatgt cgatcacgtg acgcccgccg ccctctatac gatgggaacg
     3541 gtagcccgca tcgcgcagct gcagcgcggc ctgaacggcg tgcagttgct gctgcagggt
     3601 gagcgccgca gcacggcagt gcactatacg gagggcgaag gctatctgag cgccgtggtg
     3661 cgcgacgcac ccgatctacc gccgctcgat ccacaggacc cggcgttcgt ggccttgtac
     3721 cgtgaagtgc gggagcgtgc cgctgagctg ggacagaagt ccggccttcc cgaggaagtc
     3781 gtgaagcagg tgctcgaggg cgtgaccgac cccggccgcc tggcggacct ggtggccgga
     3841 catattgagc tcaagccggc cgagcggcag tcgttgctgg agagtctggc cgtcgaggag
     3901 cgtctgcgcc gggtgttggt ggccgtgcag cgccagatcg gtgtgctcga cgctcaagag
     3961 gacatcaagt cccaggtcca ggaggagctg ggcgagcgcc agcgcgagat gttcctgcgg
     4021 gagcaaatga aggcgatccg caaggagctc ggtgaagagg atgaggacgg ggaggtggag
     4081 gagctgcgca agcggctcga ggcattggag cttcccgaag ccgcccggaa ggaagtcgag
     4141 cgggagttgt cgcgtctgga gcgcaccagt cgggactcga tggaagccca ggtgatccgg
     4201 acatatctgg agaccgtggc cgagcttccg tggaatacgc gtgccgcaga gtcgctcgac
     4261 ctgaagcggg ccgccgcgat tctcgaagag gatcactacg gactgcagga cgtcaaggat
     4321 caggtcctgg aattcctggc ggtgcggcag ctgcggcagc ggcagggaac cggagtggag
     4381 tcgcagacgg aggagaagaa gacggaggag aggaacaagg gtgagccgaa ggcagatgcc
     4441 gaatccaagg gctcgatact cctcttcgtc gggcctcccg gggtgggcaa gacctcgatc
     4501 gccaagtcga tcgcccgggc gatgggtcgg ccgtacgtac gcatctcgct ggggggcgca
     4561 cgcgacgagg ccgacatacg cggacaccgg cgcacctacg ttggagcgat gcccgggcgc
     4621 atcattcagg gcatgaagca ggcaggggcc aagaacccgg tattcctgct cgacgaggtg
     4681 gacaaactgg ggatttccta ccagggcgat cccgccgcgg ccctgctcga ggtgctcgat
     4741 ccggcgcaga acgactcgtt caccgatcac tacctcgggg tgccgttcga cctgtcggaa
     4801 gtgctgttca tcgcgacggc caacttcccc cagaacatcc cgggtccgtt gctcgaccgg
     4861 ctcgagaccg tcgatttcgc gggctatacg gaacgtgaga agctcgagat cgccaagcgc
     4921 tacctcgtgc cgcgccagct caccgagaac gggctcgccg tcgagcagct cgcgatcacc
     4981 gacgacggcc tgggtgaaat cgtctccagc tacacgcgcg aggcgggtgt gcgccagctg
     5041 gagcgcgaga tcagcaagct gggccgcaaa gtggcacggc ggatcgccga tggaaacgcc
     5101 gagaaggtgg tggtcgaccg caaggacgtc catgacctgc tcgggcggcc caaggtccac
     5161 ccggagcaca aggccggcga ggatcaggtg ggtgtggcca ccgggatgta ctacaccccc
     5221 gtgggcggcg acatcatgtt cgtcgaggcg agtctcatgc ggggcaaggg cgacctcgtc
     5281 ctcacgggcc agctcggcga tgtcatgaag gagtcggcgc gggccgcgct tacctacgcc
     5341 aagtcgcacg ccgatcggct cgggattccg gaagtcgggc tcgccgacac ggacatccac
     5401 atccacgtcc cggcgggcgc cattccgaag gacggcccgt cggcgggggt caccatggcc
     5461 accgcgctcg tctcggcgct gtcccgccgg ccggcacgtc acgacctggc catgacgggt
     5521 gagatcacgc tgcgcggccg ggtgctcccc atcgggggcg tgaaggagaa agtgctcggt
     5581 gccgtccggg ccggcatccg caccatcgtg ctccccaagg agaacgagcc ggatctggag
     5641 gacctgcccg aagaggtgcg ccagtcgttg gaagtgcacg ccgtcgagga gctgggtgag
     5701 gtgctgtcgc tcgcgctgcg cggcgcgaag ttcgaggccg gtcaactggt gttcgacggg
     5761 ccggccccgg tcgagccgag gatggtctcg aagagctgac caacgcctcc tcaggcggcg
     5821 ggtgctcgta ccccgttcac gaccagatcc accgccgatc gtcgccaccg ttcccgcaat
     5881 gcgggttcgt cggtggcgag cggcggcgcg cttgcggcga tgatcggctc taggcgcagg
     5941 tacccgagca tcaggaccag cgcctgcgcc gccgcaacgt gggggtcgag gtcgtcgcgc
     6001 accaggccga gtccctgccc ttcggccagc aacactacca gccgacggat cggcgcggcg
     6061 aacggcgcca cgtcgagccg ctcggcgtgc gagagcagcg cgacgagctg ggggtggcgc
     6121 tccaggaact cgaacagggc cgcgaggacc tccctcagcc gctccaacgg tgttcggcct
     6181 tccccggaga tcgtcgccag cgccgcttcg agatcgttca cgccccgtcg caggacggtc
     6241 tgaaacagcc cccgcttgga gtggaagtag tagaacacca actgcttgtt gactcgggct
     6301 gcggcagcga tccgctccac ccgggcaccc gcaaagcccc gcttggcgaa ctcctccgcg
     6361 gccgcgacgc ctattcgcaa tgccctgtcg ttacttagct tacgtggcac ggccaatatt
     6421 ctcaaccgtt tggttgatga aatattacgc gcgcagaggg agttgcgcca ggtcaacgag
     6481 cggcagcgat attggccggc ggtgtggaga gaccgtgctc caggaacccg atcagggcct
     6541 ggttgaacgc ctgtgggctt tcctcgggca cagcatgtcc ggcgtcgggg aagcgcacca
     6601 gcagcgcacc ggggatctgg tccgccatgg actgacctac ggcggccgga atcagccgat
     6661 ccgcggtccc ccacacgacc agcgtgggtg tcgccacctg tcccagccga tccttgagtg
     6721 cgtcgaaccg gaactggccc agcacgcccc gtaggcaacg tccgaagccg ggctcagcga
     6781 gcggggcgta gtactggtcg atatctgcgg cggtgactcg cgacggaacc gcgtagaggg
     6841 ccttgaggat tcctgccgtc gcagcgcggc ctcgcagccg atcgaacagg gcccccacga
     6901 tgggccagtg tgccacccgc agcatgaagg gccaacgcac cgccagtccg gccgc
//
LOCUS       DBNGZW010000075         3567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_413973, whole genome shotgun sequence.
ACCESSION   DBNGZW010000075 DBNGZW010000000
VERSION     DBNGZW010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3567
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_413973"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1064
                     /locus_tag="AC16JC_01910"
     CDS             <1..1064
                     /locus_tag="AC16JC_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lyase family protein"
                     /protein_id="HIR5163483.1"
                     /translation="LLGGKRGEYKPVHPNDHVNMAQSTNDVIPTAIRLGALSLLPALL
                     AALDRLAAAFLAKGRAFDDILKSGRTHLQDATPIRLGQEFTAYGHTVQRNRDRIARAA
                     DDLRDLGIGGTAVGTGLNAEPAYPGFMVRHLRTITGLELREGTDRVQLMQSMGDACAL
                     SGALRTYAVDLGKIASDIRLLASGPRTGLAEIRLPAVQPGSSIMPGKVNPSVAEMVNM
                     VCFQAIGNDVTIATAAEAGQLELNVMMPLIAQNLFLTIGILSSASHVLAERCVEGIEA
                     DQAMAAHWLDRSPALVTALAPKIGYAAAAKLAKEAVEKNLSVRELLLQKGILKGKELD
                     EVLDLRAMTDIGVRGLGGG"
     gene            1091..1504
                     /locus_tag="AC16JC_01915"
     CDS             1091..1504
                     /locus_tag="AC16JC_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163484.1"
                     /translation="MSWWLAGLIAGVAAYFADYFMWGKVFTKGMDLFVTPPPAGQAVN
                     MGPMLGKSAVLSLIFGLLLAWLYRRFMLALWVSGGGPLAGMEFATVLWLPTIALSTIG
                     GGIWYDKARPLLNATFWSWLVRMNVAGIVVGLFVK"
     gene            1565..2731
                     /gene="moeB"
                     /locus_tag="AC16JC_01920"
     CDS             1565..2731
                     /gene="moeB"
                     /locus_tag="AC16JC_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HIR5163485.1"
                     /translation="MTARTPAPALPLSHEELIRYSRHLALPAIGLEGQERLKAARVLI
                     VGAGGLGSPAALYLAAAGVGTLGLVDWDVVDRSNLQRQILHGTGMVGRPKLDSARARI
                     ADLNPHIRLETISARLTSANALEILGGWDVVLDGSDNFPTRYLVNDACVLSGRPNVYG
                     AVLRFEGQVSVFDARRGPCYRCLYREPPPPELVPSCAEGGVLGVLPGIIGSLQALEAI
                     KLVTGVGVSLVGRLVLFDGLKLQFREIALTKDPDCAVCGPHPTVTRLIDYEAFCGTGD
                     EGRGTRDGWDIGPTQLQAERERNKNLVLLDVREPFEWDIAHIDGARLIPLGQLPARLR
                     ELDGHADIVAYCHHGMRSLQAVEVLRAAGFSKVRNLDGGIDAWAREVEPNLPRY"
     gene            complement(2753..2836)
                     /locus_tag="AC16JC_01925"
     tRNA            complement(2753..2836)
                     /locus_tag="AC16JC_01925"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2800..2802),aa:Leu,seq:cag)
     gene            2933..3385
                     /gene="smpB"
                     /locus_tag="AC16JC_01930"
     CDS             2933..3385
                     /gene="smpB"
                     /locus_tag="AC16JC_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019851894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR5163486.1"
                     /translation="MNKTSIARNPRARHDYHLLETWEAGLVLTGTEVKSLRDGKASLG
                     ESYAHVQRGEVWLDGLTISPYIHGSYNNPPPTRSRKLLLHKREIRKLIGATTQQGLTL
                     IPLELYFKDGVAKVAIALAKAKKLHDKREDLKRRAAQRELAREFRARR"
     gene            3385..>3567
                     /locus_tag="AC16JC_01935"
     CDS             3385..>3567
                     /locus_tag="AC16JC_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163487.1"
                     /translation="MLPALLLLLGAGGGESLRIATPRGEFSVPLSREHGVAVAAPLIA
                     NALGLAVVVEGARATVV"
BASE COUNT          562 a         1170 c         1241 g          594 t
ORIGIN      
        1 agctgctcgg cggcaaacgg ggcgaataca agccggtgca cccgaacgat cacgtcaaca
       61 tggcgcagtc caccaacgac gtgattccga cggcgatccg gctcggcgcc ctgagcctcc
      121 tgccggccct gctcgccgcc ctggatcggc tcgctgccgc gttcctggcc aagggcaggg
      181 cgttcgacga catcctcaaa tcggggcgca cgcatctcca ggacgcgacg ccgattcgcc
      241 tcggccagga attcacggcg tacgggcaca cggtccagcg caaccgcgac cgcatcgccc
      301 gcgccgccga cgatctgcgc gacctgggga tcggcggcac ggcggtgggc acgggcctga
      361 acgccgagcc cgcctatccc ggcttcatgg tgcggcacct gcggaccatt acgggcctcg
      421 aactgcggga gggaaccgac cgcgtgcagc tgatgcagtc catgggagac gcctgcgcgc
      481 tgtcgggcgc gctgcggacc tacgcggtgg atctcggcaa gatcgcgagc gacatccggc
      541 tgctcgcctc ggggccgcgg accgggctcg ccgagatccg gctgccggcc gtgcagcccg
      601 gatcgagcat catgcccggc aaggtgaatc cgtcggtcgc cgaaatggtg aacatggtct
      661 gtttccaggc gatcggaaac gacgtgacga tcgcgaccgc ggccgaagcg ggccagctcg
      721 agctgaatgt gatgatgccg ctgatcgctc agaacctgtt cctcaccatc gggattctct
      781 cgtccgccag ccacgtgctc gccgagcgct gcgtcgaggg catcgaggcg gaccaggcca
      841 tggccgcgca ctggctggac cgcagcccgg ccctcgtgac ggcgttggcg ccgaagatcg
      901 ggtacgcggc agcggcgaag ctcgccaagg aggccgtgga gaagaacctg tcggtgcgcg
      961 agctgctgct gcagaagggc atcctgaaag ggaaggagtt ggacgaggtg ctcgacctgc
     1021 gcgccatgac cgacatcggc gtgcgcggcc tcggaggcgg ttgagcgtac tccctaccat
     1081 ggaggtggcg atgagctggt ggctcgcggg gttgatcgcc ggtgtggccg cgtatttcgc
     1141 cgattatttc atgtggggca aggtattcac caagggcatg gacctgttcg tgacgccgcc
     1201 gccggcgggc caggccgtga acatggggcc gatgctgggg aagtcggcgg tgctgtcgct
     1261 gatcttcggt ctcctcctgg cctggctcta ccggcgcttc atgctggcgc tctgggtgag
     1321 cggtggcggc ccgctggcgg gaatggagtt cgcgacggtg ttgtggctgc cgacgatcgc
     1381 gctgtcgacc atcggcggcg gcatctggta cgacaaggct cgcccgctcc tcaacgccac
     1441 cttctggtcg tggctcgtgc gcatgaacgt ggcggggatc gtcgtcgggt tgttcgtgaa
     1501 gtaggcgtcg gagcgcgtcg cgccggcggt gcttgcgccg cggttatatt cgtgtcgtgg
     1561 tccgatgact gcccgcactc cggcgcccgc gctcccgctc tcgcacgaag agctgattcg
     1621 ctactcccgt cacctggcgt tgcccgcgat cgggctcgag ggacaggagc ggctcaaggc
     1681 cgcgcgcgtc ctcatcgtgg gcgcgggcgg cctcggctcc ccggcggccc tgtacctggc
     1741 tgcggccggc gtcggcaccc tgggactggt ggattgggat gtggtggacc gctcgaacct
     1801 gcagcgccag atcctccacg gcaccggaat ggtcggccgc cccaaactcg attcggcgcg
     1861 cgcccgcatc gccgacttga acccccacat ccgtctcgag acaatctccg cccggctcac
     1921 ctcggcgaac gccctcgaga tcctcggcgg ctgggatgtc gtgctcgacg gcagcgacaa
     1981 ttttcccacc cgctatctcg tcaacgacgc gtgcgtcctg tcgggacggc ccaacgtcta
     2041 cggcgccgtg ctgcgcttcg aaggccaggt ctcggtgttc gatgcgcggc gcggtccctg
     2101 ctatcggtgt ctgtatcgag agccgccccc gcccgagctc gtcccctcgt gcgccgaggg
     2161 cggcgtgctg ggtgtgctgc ccggcatcat cggcagcctc caggcgctcg aggccatcaa
     2221 gctggtgacg ggggtcggcg tctcgctcgt ggggcgattg gtgttgttcg acggtctcaa
     2281 gctccagttc cgtgaaatcg cgctcaccaa ggaccccgac tgcgcggtgt gcggtcccca
     2341 tccgacggtg acccgcctga ttgactacga agcgttctgc gggacggggg acgagggacg
     2401 agggacgagg gacgggtggg acatcggacc cacccagctc caggcggagc gtgagcgcaa
     2461 caagaatctc gtgctgctcg acgtgcgcga gccgttcgag tgggacatcg cgcatatcga
     2521 cggggcgcga ctcatcccgc tcgggcaatt gccggcgcgc ctgcgcgagc tcgacgggca
     2581 cgccgatatc gtcgcgtatt gtcatcacgg gatgcgatcg ctgcaggcgg tggaggtgct
     2641 gcgggcggcc ggattctcga aggtgcggaa tctggacggg gggatcgatg cctgggcgcg
     2701 ggaagtggag ccgaacctcc ctcgttacta gccgatagcc gatagccatc agtgccgaag
     2761 gggggactcg aacccccacg ggcttgcgcc cactggctcc tgaagccagc gcgtctacca
     2821 gttccaccac ttcggcgaat ggttcggaac ggcgaatata gaaggcggta taacttcaag
     2881 tcaatgttcg gcttgaccct ccgagacggg tctcctatct tggctcggtg acttgaacaa
     2941 gacctccatc gcgcgcaatc cgcgggcgcg tcacgactat cacctcctgg agacatggga
     3001 ggcgggcttg gtgctgacgg gtaccgaggt caagtcgttg cgtgacggga aggcgagcct
     3061 gggcgagtcc tacgcccacg tccagcgggg cgaagtctgg ctcgatggcc tgacgatctc
     3121 cccctatatc cacggcagct acaacaatcc accgccgacc cgatcccgca agctgttgct
     3181 gcacaagcgc gagattcgca agctcatcgg agccaccacg caacaaggtc tgaccctgat
     3241 ccccctggag ctgtacttca aggatggggt cgcgaaggtc gcgatcgccc tcgccaaggc
     3301 gaagaagctg cacgacaagc gcgaggatct caaacggcgc gccgcccagc gggagctggc
     3361 gcgcgagttc agggcacgcc gctgatgctc ccggcgctgc tgctcctgct cggggccgga
     3421 ggaggggaga gcctccgcat cgccacaccg cgcggcgagt tcagtgtgcc gctgagccgg
     3481 gagcacggcg tagcggtcgc tgcaccgctg atcgcgaacg ccttggggct cgccgtcgtg
     3541 gtggaaggcg cccgcgccac agtcgtg
//
LOCUS       DBNGZW010000076         2001 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_484886, whole genome shotgun sequence.
ACCESSION   DBNGZW010000076 DBNGZW010000000
VERSION     DBNGZW010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_484886"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="AC16JC_01940"
     CDS             <1..181
                     /locus_tag="AC16JC_01940"
                     /inference="COORDINATES: protein motif:HMM:NF027964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5163488.1"
                     /translation="ARGISAMMRTTGYSLSITGLMQADGRITARGVHTPDEAVPFEAY
                     VGELKRRGVEIREVV"
     gene            253..816
                     /locus_tag="AC16JC_01945"
     CDS             253..816
                     /locus_tag="AC16JC_01945"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR5163489.1"
                     /translation="MDFRALWERALPYHQFVQEAAGNHDLWTGMYRIARIPDWALAAA
                     RSTGTKHHLLVIAEDWCGDGTNTVPYLAKLADQSRSQEVRVLRRDEHPEVMDRYLTAG
                     TRAIPIVIVLDREFREVGHWGPRPEALQAWVREARKSVDRAKLYPQIRRWYARDKGES
                     TLREVVELLEKQPSSVVGNRSLQAVIG"
     gene            complement(851..>2001)
                     /locus_tag="AC16JC_01950"
     CDS             complement(851..>2001)
                     /locus_tag="AC16JC_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR5163490.1"
                     /translation="LSVRRLVPVLVTALVGSRLAAQTAREPTMWDYTVTPFGYYSAID
                     GWWLAGYGRVYSPIGYRERPEPNRAGVTLTGGASTKGSYLVELDAQAPALWDGWRVGL
                     TLDALRANRLGYFGIGNGSSYDQDSVTPTTPYFYRVSRSSKLARLTIQRRVAGPLRVL
                     VGGTLEHTTFRTLPGDGLFARDRASGAADTAPFDDAALRAGLVADSRDNETDPHAGIL
                     AEALIAGARHYSRTTVGIQGYVHPFERLVLAGRILGERMSGAPSISTMQVIESSGRPY
                     VGVGGYRSLRGYYDSRFIGPGKLLGGIEARYALLYAPTLFEVKVVGFYDVGRVFASGD
                     ALSWTTHGLHSGGGGELMVRFGRNTVLVGGAGFGNEGWQFLFGSTWSY"
BASE COUNT          323 a          687 c          694 g          297 t
ORIGIN      
        1 cgcgcgcggc atcagcgcga tgatgcggac caccgggtac tcactgtcca tcaccggact
       61 gatgcaggcg gacgggcgga tcacggcgcg gggcgtgcac acccccgatg aagccgtgcc
      121 cttcgaagcc tatgtcggcg agctgaagag acgcggcgtg gagatccggg aagtcgtctg
      181 attcgcggtc atgaagggtg cttcacactg ccttgtgaat gccttgtcgc ccgcgacgct
      241 tccgttcgcc gcatggactt tcgcgcgttg tgggagcggg cgctccctta ccaccagttc
      301 gtccaggaag ccgccggcaa ccacgatctg tggacgggga tgtaccgcat cgcccgcatt
      361 ccggactggg ccctggcggc cgctcgctcc accggaacga agcaccacct cctggtgatc
      421 gccgaggact ggtgcgggga cggcacgaac accgttccct atctggccaa gctcgcggac
      481 cagtcccgca gccaggaagt gcgggtgctg cgccgtgacg agcatccgga ggtgatggac
      541 cgctatctca cggcgggcac gcgcgcgatt cccatcgtga tcgtgctgga ccgcgagttc
      601 cgggaagtgg gacactgggg accgcgcccc gaggcgctgc aggcctgggt gcgggaagcc
      661 cgcaagagcg tcgaccgggc caagctctat ccgcagattc gccgctggta cgcgcgggac
      721 aagggcgagt cgacgctgcg ggaggtggtg gagctgctgg agaagcagcc gtcgtcggtg
      781 gtcggtaatc ggtcattaca ggccgtcatc gggtgaccga cgaccgatta ccacgtaccg
      841 ttgacgccgg tcagtagctc cacgtgctcc cgaacaggaa ctgccagcct tcattgccga
      901 acccggcgcc gccgacgagc accgtgttgc gcccgaagcg gaccatcagc tcgccaccgc
      961 cgcccgagtg caagccgtgt gtcgtccagc tcaacgcgtc gccggaggcg aacactcggc
     1021 ccacgtcgta gaaccccacc accttcactt cgaagagggt cggcgcgtac aggagggcgt
     1081 agcgcgcctc gatgccgccc agcagcttgc ccgggccgat gaaccgcgaa tcgtagtagc
     1141 cgcgcagcga gcgatatccg cccacgccca cgtacggccg ccccgacgac tcgatcacct
     1201 gcatggtcga gatgctgggc gcgccgctca tgcgctcgcc caggatccgg cccgccagca
     1261 ccaggcgctc gaagggatgg acgtacccct ggatcccgac cgtcgtgcgg ctgtagtgcc
     1321 gcgccccggc gatcagcgcc tcagcgagga tgcctgcatg cggatcggtt tcgttgtcgc
     1381 gcgaatcggc cacgaggccg gcgcgcagtg cggcgtcgtc gaatggggcg gtgtccgcgg
     1441 cgcccgaggc ccggtcgcgg gcgaacagcc cgtcgccggg gagggtgcgg aaggtggtat
     1501 gctcgagcgt tccccccacc aggacacgca gcggacccgc cacgcggcgc tggatcgtga
     1561 gccgggcgag cttcgagctg cgactcaccc ggtagaagta cggggtcgtc ggcgtgacgc
     1621 tgtcctggtc gtacgagctg ccgttgccga tgccgaaata ccccaggcgg ttggcgcgca
     1681 gcgcgtcgag ggtgagtccc acgcgccacc catcccacag cgccggcgcc tgggcgtcga
     1741 gctcgacgag gtagctgccc ttggtgctgg ccccgccggt gagcgtgacc ccggcccggt
     1801 tcggctcggg acgctcgcga tacccgatcg ggctgtacac gcgcccgtag ccggccagcc
     1861 accacccgtc gatggcgctg tagtagccga agggggtgac cgtgtaatcc cacatggtcg
     1921 gctcccgcgc cgtctgggcg gcaaggcggg agccgaccag cgcggtcacg agcactggca
     1981 ccaatcgtcg cacgctcaac t
//
LOCUS       DBNGZW010000077         2575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_211186, whole genome shotgun sequence.
ACCESSION   DBNGZW010000077 DBNGZW010000000
VERSION     DBNGZW010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2575
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_211186"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1815)
                     /locus_tag="AC16JC_01955"
     CDS             complement(<1..1815)
                     /locus_tag="AC16JC_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR5163491.1"
                     /translation="MRIQRLGVSDALRSLRSDAQGLSSAEAVRRMEEYGPNIVQEIRG
                     QSLVVRFLKQFTHFFALILWVAAALAFVVARNQPGGGMATLGYAILGVIFVNGAFSFW
                     QEYRAERAIAALRRLLPQQAKVLRDGKLTVIPAIQLVPGDVLLLEEGDVVPADCRLLE
                     GAGIQVNAAAITGESVPLSRTAAPSEHDDVVRATNVLLAGTSLVRGQGRALAFATGMH
                     TEFGKIAGLTQMVVEPPTPLQDEIARLSRLVAIISTSLGVVFFAIGQVLGLPFWGNLT
                     FAIGIIVANVPEGLLPTVTLALAAGARRMARRNALVRHLPSVEALGAVTVICTDKTGT
                     LTTNRMTVRRLYLSGRFLDDGPQVARAVQAGSLERRLFEAAQLCHDLKSVERDGRTQL
                     LGDPMEVALVESATAVVPDLVDGGRLDEIPFDGDRKRLAVLHRTGDGILLYVKGALET
                     ILPLCRNVVHEAGTRDLTPEAGERILAAQHSMADAGLRVLAFAYRPVAEPCERDALES
                     DLILSGLIGLEDPPRPEVPDAIRRCREAGIRVIMITGDHPSTAVAIGREIGLLTSAEP
                     LVYRGEDLRRVSDTQLQLALDAPEIVFARVNPDQKMRIV"
     gene            1949..>2575
                     /locus_tag="AC16JC_01960"
     CDS             1949..>2575
                     /locus_tag="AC16JC_01960"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5163492.1"
                     /translation="MTEKSLRNIFIVGTVLFLGVLVVMSRDSVSQLVSGRTPPLTPDV
                     AAGKQVWQARNCNDCHTIIGIGGYYAPDLTKVVERRDPVWLAGWLADPRAVNPATTMP
                     NQRLAAGDVTALVSFFQWVGGINTNDWPPKPRLSLGGVDTASGAVVFQQKGCSACHRI
                     AGKGPVGPGPDLTRIGATPYDGLPNTPEFLGKWLENPQAQKPATVMPRI"
BASE COUNT          415 a          895 c          875 g          390 t
ORIGIN      
        1 cacgatccgc atcttctggt cgggattcac ccgggcgaac acgatctcgg gggcatcgag
       61 tgcgagctgc agctgcgtat cgctcacccg ccgcagatcc tcgccgcggt acacgagcgg
      121 ctcggccgag gtgagcagtc cgatctcccg cccgatcgcc accgcggtgg acgggtggtc
      181 gccggtgatc atgatgaccc ggatccccgc ctcgcggcag cgccggatcg cgtcgggtac
      241 ttccgggcgc ggcggatcct ccagcccgat caggccgctc aggatcagat cggactcgag
      301 ggcgtcccgc tcgcagggtt ccgcgaccgg ccggtacgcg aaggcgagaa cccggagccc
      361 ggcgtcggcc atggagtgct gcgccgccag gatccgctcg cccgcctcgg gggtcagatc
      421 ccgcgtcccg gcctcgtgca cgacgttccg gcacaacggc aagatcgttt ccagggcgcc
      481 cttcacgtag agcaggatcc cgtcgccggt gcgatgcagc acggccaggc gcttgcggtc
      541 cccgtcgaag gggatttcgt cgagtcgtcc gccatccacg aggtccggaa ccacggcggt
      601 cgcactctcg accagcgcga cctccatcgg gtctccgagc agctgcgtcc ggccgtcacg
      661 ttcgacggac ttcagatcgt gacacagttg cgccgcttcg aacagccggc gctcgaggct
      721 ccccgcctgt accgctcgcg cgacctgcgg cccgtcatcg agaaaccgtc ccgacaggta
      781 gagccggcgc acggtcatgc ggttggtcgt cagggttccg gtcttgtcgg tgcagatgac
      841 cgtgaccgcg ccgagcgcct cgaccgacgg caggtgccgg accagggcgt tgcgccgggc
      901 catgcggcgc gcgccggccg cgagggcgag cgtcacggtc ggcagcaatc cttcggggac
      961 attcgccacg atgatgccga tcgcgaacgt caggtttccc cagaacggga gacccagcac
     1021 ctgaccgatg gcgaagaaga ccacgcccag ggaggtcgaa atgatggcga cgagccggct
     1081 caagcgcgcg atctcgtcct gcagcggggt ggggggctcc acgaccatct gggtcagccc
     1141 ggcgatcttg ccgaattccg tatgcatgcc ggtagcgaag gcgagcgcgc gcccctggcc
     1201 cctgacgagg gacgtgcccg cgagcaggac gttcgtcgcg cgaaccacgt cgtcgtgttc
     1261 gctcggggcg gcggtccgcg acaggggcac cgattccccg gtgatggccg ccgcgttgac
     1321 ctggatcccg gcgccctcga gcaggcggca gtccgccggg accacatcgc cttcttcgag
     1381 gagcagcacg tcgcccggca ccagctggat ggcgggaatg acggtgagtt tcccgtcccg
     1441 cagcaccttg gcctgctgcg gcagcagccg gcgcaacgcg gcgatggcgc gctcggcgcg
     1501 gtactcctgc cagaagctga aggcgccgtt gacgaagatc acgccgagaa tcgcgtagcc
     1561 gagcgtcgcc atgcccccgc ccggctgatt gcgcgccacc acgaaggcca gcgcggcggc
     1621 gacccacagg atcagggcga agaaatgcgt gaactgcttc aggaagcgga cgacgagtga
     1681 ctggccgcgg atctcctgca cgatattggg gccgtattcc tccatccgcc gcacggcctc
     1741 ggccgacgac aggccctgcg catcgctgcg caagctgcgc agcgcgtccg acacgccgag
     1801 gcgctggatt ctcacggtct ccaaatctac cgctcgccct tgccggcggt cctcgatgac
     1861 aaccccctca ccgcaagctc agcgtcggtt cacgtcccat cgaagacatt gagcggcggc
     1921 aggtactcgg ctcgagcggg agggggccat gaccgagaag tcgctccgca atatcttcat
     1981 cgtcggcacg gtgctgttcc tcggcgtgct cgtcgtcatg agccgggatt ccgtctccca
     2041 gctcgtatcg gggcgcacac cgccgctcac gccggacgtc gccgcgggca agcaggtatg
     2101 gcaggccagg aactgcaacg actgtcacac gatcatcggg atcggaggct attacgcgcc
     2161 cgatctcacg aaagtggtgg agcggcgcga tccggtctgg ctggccggct ggctcgccga
     2221 tccgcgagcc gtgaatcccg cgacgacgat gcccaaccag cggctcgccg cgggcgatgt
     2281 gactgcgctc gtgtcgttct tccagtgggt gggcggcatc aacacgaacg attggccccc
     2341 caagccacgt ctcagcctcg gcggcgtgga taccgcgagc ggtgcggtgg tcttccagca
     2401 gaaggggtgc tccgcctgcc atcggatcgc ggggaagggc cccgtcggac cgggtcccga
     2461 cctgacccgc atcggtgcta ctccctatga cgggctgccc aacacgccgg aatttctggg
     2521 gaagtggctg gagaacccgc aggcgcagaa gcctgctacg gtgatgccgc ggatc
//
LOCUS       DBNGZW010000078         2894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1167000, whole genome shotgun sequence.
ACCESSION   DBNGZW010000078 DBNGZW010000000
VERSION     DBNGZW010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1167000"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="AC16JC_01965"
     CDS             <1..633
                     /locus_tag="AC16JC_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163493.1"
                     /translation="LIGDVAAEPLLSDLLRAAPQHPFANLALGRLLLDRDDEAGLALV
                     GLAMERHPACRQPAYELLIAFYERQGRREEAERQRRRAWSHGDQLALADVERQGVTGS
                     DTFTAHGLGPEEARRIAEHLLAFPEIAEAYLVQKEVRHFTDMPLYVLGVRLRRPWYRF
                     QSARWTAAVLRRIATEIPFSGQSFVVPLGPETRRIEKKLKRIEGARLLPR"
     gene            complement(596..1513)
                     /locus_tag="AC16JC_01970"
     CDS             complement(596..1513)
                     /locus_tag="AC16JC_01970"
                     /EC_number="3.5.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000118 - histone deacetylase complex
                     [Evidence IEA];
                     GO_function: GO:0004407 - histone deacetylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR5163494.1"
                     /translation="MKAYYTDHFVLPLPAGHRFPMAKYGMLRARVAAAHLASLEVPAA
                     VTDADLERVHTPEYVRSVSSGTLDPAAQRAIGFPWSPDMVERSRRSAGATLAAARQAL
                     TDGVAVNLAGGTHHAFPDHGEGFCVFNDAAVAVRALRAEALARRVLIVDCDVHQGNGT
                     AAIFAGDERVFTLDLFAEKNYPFRKVSPDLAVPLPDGGDDALYLDRLTDVLPRAIAAS
                     RPDLAIYVSGADPHAGDRLGRLRLTKEGLEERDRTVLSMLLRQGVPVAVTMAGGYGRD
                     LQDTVDIHFATVRQAARFSAGAGARPRSV"
     gene            1603..2289
                     /locus_tag="AC16JC_01975"
     CDS             1603..2289
                     /locus_tag="AC16JC_01975"
                     /inference="COORDINATES: protein motif:HMM:NF016764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HIR5163495.1"
                     /translation="MGAALDFFNVLHEPSAVFARVKERPRVLVPWLIISVLLIGVGYL
                     GRPFQAVAMESLRASLPPEQAARMGAASGPSILRIAISAPLIAIVALALGAGMLWVGV
                     SLTGGAARYKTLMSVLAYSYVTYIIFSAVTTVVLMVKGIGAVTSFEDLRAPLGLDLLV
                     PGAGLFLGTILNGINPFSIWGVWLCATGISITQGTSRASATVVTVCTFAVALVLQAAP
                     LLLFGMATRR"
     gene            complement(2279..>2894)
                     /locus_tag="AC16JC_01980"
     CDS             complement(2279..>2894)
                     /locus_tag="AC16JC_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012258815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HIR5163496.1"
                     /translation="QARAIFEAACRVVARGKKAVVEVMIPLVAVVEELRRQAAIVRRV
                     AQEVFHAQGHAVPYLVGTMIELPRAALTADEIAREAEFFSFGTNDLTQTTFGLSRDDA
                     GRFLPFYVETGILKDDPFQVLDQDGVGRLVELGVKLGRGTRNDLKVGICGEHGGEPSS
                     VKFCHHAGLNYVSCSPFRVPIARLAAAQAALEEAGVINRTKATV"
BASE COUNT          428 a          942 c         1018 g          506 t
ORIGIN      
        1 ttgatcgggg atgttgccgc ggagccgctg ctgagcgact tgcttcgtgc ggcgccgcag
       61 caccccttcg cgaacctcgc cttgggtcgc ctgttgctcg atcgggacga cgaggcgggg
      121 cttgcgctgg tcggcctcgc catggaacgg catcccgcct gtcggcagcc ggcgtacgag
      181 ctgttgatcg ccttctacga gcgccagggc cgcagggagg aggcggagcg tcagcggcga
      241 cgggcctggt cgcacgggga tcagctggcg ctggccgacg tcgagcggca gggggtcacc
      301 ggctccgata cgttcacggc gcacgggcta gggcccgagg aggcgaggcg catcgccgag
      361 cacctgctgg cctttccgga aatcgcggag gcctatctgg tacaaaagga ggtgcgccac
      421 ttcaccgaca tgccgctgta cgtcctcggc gtgcggttgc gacgcccctg gtatcgtttc
      481 caatcggccc ggtggaccgc ggccgtcctg cggcgcatcg cgaccgagat cccgttctcc
      541 gggcagtcct tcgtcgtgcc gctgggtccg gagacgcggc ggatcgagaa gaagctcaaa
      601 cggatcgagg gcgcgcgcct gctcccgcgc tgaaccttgc ggcctgtcgc accgtcgcga
      661 aatggatgtc gaccgtatcc tgcaggtccc gcccgtatcc gcccgccatc gtgaccgcca
      721 cgggtactcc ctgacggagg agcatgctca gcacggtgcg gtcgcgctct tccaggcctt
      781 ctttggtgag tcgcaggcgg cccagccggt cgccggcatg cggatccgcc ccggacacgt
      841 agatcgcgag gtcggggcgg gacgcagcga tggcgcgggg caacacgtcc gtcaggcgat
      901 cgaggtagag cgcgtcgtcg ccgccgtcgg ggagcggcac ggcgagatcg ggggatactt
      961 tccggaacgg atagttcttc tcggcgaaca ggtcgagcgt gaacacgcgc tcgtctccgg
     1021 cgaagatggc ggcggtgccg tttccctgat gcacatcgca atccacgatc agaactcggc
     1081 gggcgagagc ttcggcccgc agggctcgca ccgccaccgc ggcgtcgttg aagacgcaga
     1141 agccctcgcc gtggtcgggg aaggcgtggt gcgtcccgcc ggcgaggttg accgcgacgc
     1201 cgtcggtgag ggcctggcga gcagcggcga gggtcgctcc cgcggagcgg cgcgaacgct
     1261 ccaccatgtc gggcgaccag gggaaaccga tggcgcgttg cgcggccgga tccagggtgc
     1321 cggacgacac gctcctgacg tactccggcg tgtgcacgcg ctcgaggtcg gcgtcggtga
     1381 ccgcggcggg gacttcgagc gacgccaggt gcgccgcggc cacccgggcg cgcagcatcc
     1441 cgtacttcgc catcgggaag cggtgaccgg cggggagcgg gagcacgaag tggtccgtgt
     1501 agtaggcttt cacttcgagg cgtatattgg ggctccaacg gcgggccggg aagcgtcggt
     1561 gatcgccggc ggccgactat caccccctgt ttggagggcc ggatgggcgc tgcgctggat
     1621 ttcttcaatg tgctgcacga accgtcggct gtatttgccc gcgtgaagga gcggccgcgc
     1681 gtgctggttc cgtggctcat catttccgtg ctgttgatag gagtgggtta tctcggtcgc
     1741 ccctttcagg ccgtagccat ggagtccctg cgggcctccc taccgccgga acaggctgca
     1801 cggatgggcg cagcgagtgg gccctcgatc ctccgaattg ctattagtgc accgctgatt
     1861 gccatagtcg ccttggcact cggtgccggg atgctctggg tgggcgtatc gcttacgggt
     1921 ggagcggctc gttacaagac tctgatgagc gtccttgcct actcgtatgt gacttacatc
     1981 attttctctg ccgttacaac ggtggtcctg atggtcaagg gcataggcgc cgttacctct
     2041 ttcgaagatc ttcgtgcgcc actaggcctc gacctacttg tgccaggagc gggcctgttc
     2101 ctcggcacga ttctgaatgg gatcaacccg ttttccatct ggggcgtgtg gctgtgcgcg
     2161 accggtatct cgattacaca gggaacgtcg cgtgcgagtg caacggtcgt gactgtgtgc
     2221 acattcgctg tcgcgctcgt cctgcaagcg gctccgctgc tgctgttcgg gatggcgact
     2281 agacggtagc cttggtgcgg ttgatgacgc ccgcctcctc gagggccgcc tgggccgcgg
     2341 cgaggcgggc gatcggcacc cggaacgggg agcacgagac gtagttgagc ccggcgtggt
     2401 ggcagaactt caccgacgac ggctcgcccc cgtgctcccc gcagatcccc accttcaggt
     2461 cgttgcgcgt gccgcgcccc agcttcacgc ccagctcgac cagccggccg accccgtcct
     2521 ggtccaggac ctggaacgga tcgtccttca ggatcccggt ctccacgtag aacggcagga
     2581 accgaccggc gtcgtcgcgg gagaggccga aggtcgtctg cgtgagatcg ttggtgccga
     2641 acgagaagaa ctccgcttcg cgggcgatct cgtcggcggt gagcgccgca cgcggcagct
     2701 cgatcatcgt gcccacgagg tacggtacgg cgtgcccctg ggcatgaaac acctcctgcg
     2761 ccacgcgacg cacgatcgcc gcctggcggc gcagctcctc gaccacggcg accagcggga
     2821 tcatcacctc gacgaccgct ttcttcccgc gcgcgacgac gcggcaggcc gcctcgaaga
     2881 tcgcccgggc ctgc
//
LOCUS       DBNGZW010000079         6140 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1848772, whole genome shotgun sequence.
ACCESSION   DBNGZW010000079 DBNGZW010000000
VERSION     DBNGZW010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6140)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6140)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6140
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1848772"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1059)
                     /locus_tag="AC16JC_01985"
     CDS             complement(34..1059)
                     /locus_tag="AC16JC_01985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163497.1"
                     /translation="MTDTGRPLSMYTTCAFCNAPLDGDGGPSGLGVGRRLAFDEWRAR
                     LWVICPKCARWNLTPLDDRLERIDALARVAHGGRLLASTEQVALLRWERYELVRVGKP
                     PRVELAGWRYGERLAARARDRAKVVVPLTLAAVGLGVVANVAVGGSFGFLIWNMGDLA
                     DRVYVGMVGRRSVVLTEPPVCDHCGTALELKAKHLQHARVVPDAHHDLGVLLSCPQCQ
                     REASLLTGPDALAALRQGLAYLNVRRSSRRKAGEAASVVDRVGGPDRLILDVARRELT
                     LRSLLPERQLALEMAVDERAEMVELERQWREAEELAEIADGTLSSDATIEEKLKQLKR
                     RGDQPSG"
     gene            complement(1063..2193)
                     /gene="thiO"
                     /locus_tag="AC16JC_01990"
     CDS             complement(1063..2193)
                     /gene="thiO"
                     /locus_tag="AC16JC_01990"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HIR5163498.1"
                     /translation="MPAGVSNTSPHVAVIGGGVIGAACARAAALRGLRVTLLAPGPDP
                     AAASGASAGMLAAQIEPTDEDLLPLAVRARDLYEDLADALKDTTGTDLGFLHTGIATL
                     AFDEEHAAALYERVAEQRQAGLRCDWLDAAELQERWPGIATDARGALLAPEDGSLDPA
                     ALCAALLADARRLGVRVLAESARRLAVAGGRVTGVATDRAAVPAEHVVIAAGAWSAQI
                     EGLPQPLPVEPIRGQMVATEWPTELPPIILYHGHGYLLPRGAEAVLGSTMESAGFDAR
                     TTADGIAEIRSGAERLCPAIGALPTRRAWAGLRPVTPDGRPILGADPAVAGLWYATGH
                     GRNGILLAGITGEIIGDLLATGQADMDIAALSIARFSAGQPN"
     gene            2239..2457
                     /locus_tag="AC16JC_01995"
     CDS             2239..2457
                     /locus_tag="AC16JC_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163499.1"
                     /translation="MFRSILGFAIFAILVWIGLKIIFGLLGGLMSIVGTVLWLAAIGF
                     IFYLVLRVVSPATANKVRDTIKGKRDTA"
     gene            complement(2488..3477)
                     /locus_tag="AC16JC_02000"
     CDS             complement(2488..3477)
                     /locus_tag="AC16JC_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015838609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSU2086 family protein"
                     /protein_id="HIR5163500.1"
                     /translation="MTEYREADFSRLRTVPIAQRRNKVDPTLLAHPPGADTSFAAFWR
                     SLPDILAARDLRAVADDIARARGRRAVVVMLGGHVIKVGLGPLLVTLLERGVVTHVAM
                     NGSAAIHDFELAAYGGTSEDVAAGLGDGTFGMAEETGRDMNAALAAGAAERWGAGEAL
                     ARALCRRTDLPGRAASVLVACAERHVPVTVHAALGAEIIHQHPTADGAVIGATSHRDF
                     KRLAGSLPDLHDGGVVLNLGSAVVMPEVFLKALSVARNLHGGRPTGFTACDCDMQRHY
                     RPRVNVVERPTQGAGGGRGVQLTGHHEILLPLLVWGVLARLDQTPTGSPSKGR"
     gene            complement(3474..4985)
                     /locus_tag="AC16JC_02005"
     CDS             complement(3474..4985)
                     /locus_tag="AC16JC_02005"
                     /inference="COORDINATES: protein motif:HMM:NF022750.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HIR5163501.1"
                     /translation="MIYVCVPVRDEAKTAGLVLWKVRQVFTAFQREYQLLVCEDASTD
                     GTGEVLARYTGVLPLTVVTHRERQGYARSVEELLRLALERTDRPKRDCAIVLPADFVY
                     SPEAMEEMVKRVESGADLVAAEQVEAAGDVPRALRWARRWTPRLLRVRGVRDSVSGFL
                     ALRLITLRQALRPEGGRPAPLLVTDGSCANAELLARLMPHARRAETIAVTGRYDLRQR
                     PSRVRPWATLMAAWRARPVIRAAGGATVALLLLAGRLDSQDSSTGSATATVTAAAAVA
                     TPARESVAFPVGERLRYSAKYGPFSVGEATMEVAGIDTVRGVETVRFRFHIQGGALWY
                     HLDQLLESWVGRADFRSRRYINDSEERGRKRHNSYEIFPDSGYYREEGVDTVKPTVPD
                     PLDDAAFLYWVRTVPLEVGKRYEFQRYFRPDRNPVIIVVEKRERISVAGRKFNAIVIR
                     PIIPKGRGIFAEQAQAHMWLSDDDRRLMLALQSNFSFGTVTLKLKEFSVPEHP"
     gene            complement(4998..6020)
                     /locus_tag="AC16JC_02010"
     CDS             complement(4998..6020)
                     /locus_tag="AC16JC_02010"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5163502.1"
                     /translation="MRVLHVDAGREWRGGQNQVRLLTREQSRDPTLEVLLATKRDGIL
                     AGRAAAAGVAVHGIPWGIGLDPRAWWRLRSLMRRFRPAIVHAHDSHALALVRAPVVIG
                     HRRVDFHVRPHSAWFRATAVIAVSAAVQRVLTNDGIPPDRITVIPDGIDPDEVRTAGA
                     QPLGIRARLGLAPSTPLAANVAALVDHKDHRTLVRAAAHARRARPDLHWVIAGEGDMR
                     HAVEREIAAHGLGDRVHLLGYIPEADALIREADVFVMSSKEEGMGSVILHALALERPV
                     VATAAGGIPEVVPAGCLVPVGDAVALGEKIVSVLTRPPSPVPLPQKFTSKSMAEATLA
                     LYRSLV"
BASE COUNT          899 a         2151 c         2225 g          865 t
ORIGIN      
        1 atagtgaggg gagggaggct ggggtaggcg atttcaacca gatggttgat cacctcggcg
       61 cttgagctgt ttcagcttct cctcgatcgt cgcatcgctg ctgagggtgc cgtccgcgat
      121 ctccgcgagc tcctcggctt cgcgccattg gcgctcgagc tcgaccattt ccgctcgctc
      181 gtccacggcc atctccagcg ccagctgccg ctcggggagc agcgagcgca gcgtcagctc
      241 gcggcgcgcc acgtcgagga tcaaccggtc ggggccaccg acccgatcga ctacgctcgc
      301 ggcctcgccc gctttgcggc ggctcgagcg gcgcacgttg aggtaggcga gtccctgccg
      361 cagggccgcc agcgcgtccg ggccggtgag cagcgacgct tcgcgctggc actgcgggca
      421 gctcagcagg acgcccaggt cgtggtgcgc gtcggggacc acgcgggcgt gctggaggtg
      481 tttcgctttc agctcgaggg ccgtaccgca gtggtcgcat accggcggct ccgtgagcac
      541 cacgctgcgg cgcccgacca tgcccacata cacccggtcg gcgaggtcgc ccatgttcca
      601 gatgagaaaa ccgaaggatc cgccgaccgc gacgttcgcc acgactccca gcccgactgc
      661 cgccagcgtc aacggcacga cgaccttggc acggtcgcgc gcgcgggccg caaggcgctc
      721 gccgtaacgc catccggcga gctcgacgcg cggcggtttg cccacccgga ccagctcata
      781 ccgctcccat cgcagcagcg cgacctgctc ggtgctggcg agcagccgcc cgccgtgggc
      841 gacacgtgcc agggcgtcga tgcgctccag ccgatcgtcc agcggcgtga ggttccagcg
      901 ggcgcacttg gggcagatga cccacagccg tgcgcgccat tcgtcaaacg ccaggcgacg
      961 gccgacgccg agcccggacg ggccgccgtc gccatcgagc ggtgcgttgc agaaggcgca
     1021 ggtggtatac atcgacagcg gtcgtccggt atcggtcatc ggtcaattcg gctgcccggc
     1081 cgagaatcgt gcgatggaaa gcgcggcgat atccatgtcg gcctggcccg tggcgaggag
     1141 atcgccgatg atctcgccgg tgattcccgc cagcagaata ccgttgcggc cgtgaccggt
     1201 ggcgtaccac agcccggcca ccgcggggtc cgcccccagg atcggacgcc cgtcgggggt
     1261 gacgggccgc agaccggccc aggcgcggcg cgtggggagc gcgccgatcg ccggacagag
     1321 ccgctcggcg ccgctccgga tctccgcaat gccgtccgcg gtcgtgcgcg cgtcgaaccc
     1381 ggcggattcc atggtgctcc cgaggaccgc ctccgcgccg cgtggcaaca ggtagccgtg
     1441 cccgtggtag aggatgatgg gggggagttc ggtgggccat tcggtggcga ccatctggcc
     1501 gcgaatcggc tccacgggaa gcggctgggg gagtccttcg atctgcgcgg accacgcccc
     1561 ggcggcgatc accacgtgct ccgccgggac ggcggcccgg tcggtggcga cgccggtcac
     1621 gcggccgccc gccaccgcga ggcggcgggc gctttccgcc agcacccgga cccccaggcg
     1681 gcgtgcgtcc gccagcaggg cggcgcacag ggccgcgggg tcgagggatc cgtcttccgg
     1741 cgcgagcagc gccccccgcg cgtcggtagc gatgccgggc cagcgctcct gcagctcggc
     1801 tgcatcgagc caatcgcacc gcagcccggc ctggcgctgc tcggcgacgc gctcgtacag
     1861 ggccgcagcg tgctcctcgt cgaaggcgag ggtcgcgatc ccggtgtgca ggaatccaag
     1921 gtccgttccg gtggtatctt tcagggcgtc cgcgaggtcc tcgtagaggt cgcgggcccg
     1981 tacggcgagc gggagcagat cctcgtcggt cggctcgatc tgggccgcga gcatgccggc
     2041 ggaagcgccg gaggcggcgg cggggtcggg gccgggcgcg aggagggtca cgcggagccc
     2101 gcgcagcgcg gcggcgcgcg cgcaggcggc cccgatgaca ccgccgccga tgacggcgac
     2161 gtgcggcgag gtgtttgaaa ctcccgcggg catcagcgcg taggctgcgg cgtcctgcgg
     2221 ggcaactcgg gaggagccat gttccggagc attctggggt tcgcgatctt cgccatcctc
     2281 gtctggatcg gcctgaagat catcttcggc ttgctgggcg gtctgatgag catcgtcggg
     2341 accgtgctct ggcttgccgc gatcggcttc attttctacc tcgtcctgcg ggtggtcagt
     2401 cccgccacgg ccaacaaggt ccgcgacacc atcaagggca agcgcgacac cgcctgatcc
     2461 tcccggcgcg gccggtcctt cacggggtca tcgtcctttg gacggtgacc ccgtcggcgt
     2521 ttgatccagc cgggcgagca cgccccacac gagcagcggc agcaggattt cgtgatgtcc
     2581 cgtgagctgg accccccgtc cgccaccggc cccctgggtg gggcgctcga ccacgtttac
     2641 ccgcggacgg tagtgacgtt gcatgtcgca gtcgcacgcg gtgaacccgg tgggccggcc
     2701 gccgtgcaga ttgcgagcga cggacagcgc cttgagaaac acctccggca tcacgacggc
     2761 gctgccgaga ttcagcacca cgccgccgtc gtggaggtcc ggcaggctgc ccgccagccg
     2821 cttgaagtcc cggtggctgg tagcgccgat gacggccccg tccgccgtgg gatgctggtg
     2881 gatgatctcc gcgccgagtg cggcgtggac ggtcaccggg acgtgccgtt cggcacaggc
     2941 cacgagcacg gaggcggcgc gtcccggcag atcggtgcgg cggcacaggg cgcgggcgag
     3001 cgcttcgccg gcgccccacc gctcagcggc tcccgcggcg agcgccgcgt tcatgtcgcg
     3061 cccggtttcc tccgccatgc cgaaggtgcc gtcgcccaga ccggcggcca cgtcctcgct
     3121 cgtaccccca taggcggcca gctcgaagtc gtggatcgcc gcgctgccgt tcatcgcgac
     3181 gtgcgtcacg accccgcgct cgagcagggt cacgagcagc ggtccgagtc ccactttgat
     3241 cacgtgcccg cccagcatga cgaccaccgc ccgacggccg cgcgcgcgcg cgatgtcgtc
     3301 cgcgaccgcc cggaggtcgc gggcggccag gatgtcggga agggagcgcc agaatgcggc
     3361 aaaactcgtg tcggcgccgg gtgggtgcgc cagcagggtg gggtcgacct tgttgcggcg
     3421 ctgcgcgatc gggaccgtcc tcaaccggct gaagtccgcc tcgcggtact cggtcatgga
     3481 tgctccggga cggagaactc cttgagcttc aaggtgaccg tgccgaacga gaaattggac
     3541 tgcagcgcca gcatgagccg ccggtcgtcg tcggagagcc acatgtgcgc ctgggcctgc
     3601 tcggcgaaga tgccgcgccc cttggggatg atcggccgga tgacgatggc gttgaacttc
     3661 cggccggcga cgctgatccg ctcccttttc tccacgacga tgatgacggg gttacggtcg
     3721 gggcggaaat agcgctggaa ctcgtagcgc ttcccgacct ccagcggcac ggtccgcacc
     3781 caatacagga aggcggcgtc gtccagggga tcgggaacgg tcggcttcac cgtgtccact
     3841 ccctcctccc ggtagtagcc ggaatccggg aagatctcgt agctgttgtg gcgcttgcgg
     3901 ccgcgctcct cggaatcgtt gatgtagcgc cgcgaccgga agtcggcgcg cccgacccac
     3961 gactccagca gctgatcgag gtggtaccac agcgcgccgc cctggatgtg gaagcggaac
     4021 cggacggtct ccacaccgcg gaccgtgtcg atgccggcca cctccatcgt cgcctccccg
     4081 acggagaagg ggccgtactt cgccgaatag cgcagccgct cgccgacggg gaaggcgacc
     4141 gattcgcgcg cgggcgtcgc gaccgcggcc gcagccgtca ccgtcgccgt cgcactccct
     4201 gtggacgagt cctgagagtc gagtcgcccc gcgagcagca gcagggccac cgtggcgccg
     4261 ccggcggcac gaatgacggg tcgcgcccgc caggcggcca tgagcgtcgc ccaggggcgc
     4321 acgcgcgatg gacgctgccg caggtcgtag cggcccgtga ccgcgatcgt ctcagcgcgc
     4381 cgggcgtgcg gcatgaggcg ggccagcagc tccgcgttgg cgcacgaccc gtccgtgacg
     4441 agcagcggcg ccggccggcc gccctccggt cgcaaggcct gccgcagggt gatgagccgc
     4501 agcgcgagga accccgagac gctgtcccgg accccgcgga cgcgcagcag ccggggcgtc
     4561 cagcgccgcg cccagcggag cgcgcgcggc acgtcgcccg ccgcctccac ctgctcggcg
     4621 gcgacgagat cggcgccgga ctccacgcgc ttcaccatct cctccatcgc ctcgggcgaa
     4681 tagacgaagt ccgccgggag cacgatggcg caatcacgct tgggacggtc ggtgcgctcg
     4741 agcgcgagcc gcagcagctc ttccacgctc cgggcatatc cctgccgctc gcgatgcgtc
     4801 acgaccgtca gcggcagcac gccggtgtag cgggccagga cctctcccgt gccgtcggtc
     4861 gaggcgtcct cgcacacgag cagctggtac tcgcgctgaa aggcggtgaa gacctggcgg
     4921 accttccaca agacgagccc ggcggtcttc gcctcgtcgc gcactggcac gcacacgtag
     4981 atcatggtgc ctgcaatcta gacgagcgag cgatacaggg ccaacgtcgc ttccgccatc
     5041 gacttagacg tgaacttctg gggaagaggg acgggggacg ggggacgggt gaggacggag
     5101 acgatcttct cccccaacgc gaccgcgtcg cccacgggaa cgagacatcc ggcaggcacc
     5161 acctcgggaa tgccgccggc cgcggtggcc acgaccggcc gctcgagcgc cagggcgtgg
     5221 aggatcacac tgcccatgcc ttcctccttg gaggacatca cgaacacgtc ggcctcacga
     5281 atcagggcgt cggcttcggg gatatacccc aggagatgga cacggtcgcc gagaccgtgg
     5341 gcggcgattt cgcgctccac ggcgtgacgc atgtcacctt cgcccgcgat gacccaatgg
     5401 agatcgggtc gcgccctgcg cgcgtgggcg gccgcgcgga ccagcgtacg gtgatccttg
     5461 tgatccacca gcgcggcgac gttcgcggcg agcggcgtgg agggcgcgag accgagacgg
     5521 gcgcggatcc cgagcggctg cgcgcctgca gtgcgcacct catcggggtc gatgccgtcg
     5581 gggatgacgg tgatgcggtc cggcgggatg ccgtcgttcg tgagcacgcg ctggaccgcc
     5641 gccgacacgg cgatgacggc ggtggcgcga aaccacgcgc tgtgggggcg aacgtgaaaa
     5701 tccacgcgac ggtggccgat gacgaccggc gcgcgcacca gcgccaacgc gtggctgtcg
     5761 tgggcgtgga cgatcgccgg gcggaagcgg cgcatcaggg agcgcagacg ccaccaggcg
     5821 cgcgggtcga ggccgatgcc ccacgggatg ccgtggacgg cgacgcctgc ggcggcggcg
     5881 cgtccggcca ggatgccgtc gcgcttcgtg gccagcagca cttcgagcgt ggggtctcgc
     5941 gattgctcgc gcgtcagcag ccgaacctgg ttctgcccgc cgcgccattc ccgccccgcg
     6001 tccacgtgca ggacgcgcac ggattactgc tgggcctcgg ccgcgacgct gcccaggacc
     6061 gtgcggagct cggccagggt gaacggtttg tggaggaagg ggcggcccag ggtctcgagg
     6121 aactgcagcg tgtcgccgcg
//
LOCUS       DBNGZW010000080         2357 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_211424, whole genome shotgun sequence.
ACCESSION   DBNGZW010000080 DBNGZW010000000
VERSION     DBNGZW010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_211424"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1383)
                     /locus_tag="AC16JC_02015"
     CDS             complement(<1..1383)
                     /locus_tag="AC16JC_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR5163503.1"
                     /translation="MIRLTLPPDLAPRERFGLEALVDLSRLLLVADEEADVTRLEITD
                     DPASLASLLQYGLPLDRGDGVVRVTRHALSGITDLAGAAVEQGSATEDRHGRVPSSEN
                     PLVRAGREREPVVQHWAAALAAAVQQAAGRRSVRLVTPWPEGRRWAAAITHDLDIVSG
                     WPLFTALRAVELARGGQWGRLTRSLGAAAAAIGRRPVQAGVQRILDTERAAGIHSTWF
                     VLCGTPTPATWLAGDITYSLESAAARRLLDRIRRDGHEIGLHGSFRSALDGTRMREER
                     ARLGRALGGSAPAGVRQHFLRMRPGPTQACARAAGFSYDATFGFPDRNGFRIGVADIL
                     PAWDSDATGSLDLVPLVWMDRALSKYRGIEDPGAWIGDGLELAAAARAARGLWVGLWH
                     PNLTPPLGFPGAPEAFHRLLQTLSADGSPPWFATLERLVSWRRARREVRAAHVTPDGR
                     VGIAATSGADW"
     gene            complement(1380..1847)
                     /locus_tag="AC16JC_02020"
     CDS             complement(1380..1847)
                     /locus_tag="AC16JC_02020"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR5163504.1"
                     /translation="MGRFLWRLRKKIFKRWARNAFFPGWRIRLLRWCGYTIGRDVYIA
                     DELLIAEELEDRGNLTLGDRVSIAPRVTLVLSSHPNDSRIRPFAPVKRAPIVIEADAW
                     LGAGVVVLPGVRIGRGAIVGANSVVTKDVPPLHVVAGLPAKTVRVLTPPPGWS"
     gene            complement(1850..>2357)
                     /locus_tag="AC16JC_02025"
     CDS             complement(1850..>2357)
                     /locus_tag="AC16JC_02025"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5163505.1"
                     /translation="ARLEERVQQGHGVPAPPRRYFLDALRGLQEAGLVDLYLARIPDG
                     RIAAGFVMFKGPRDWIYALSASDPALVRDYRPTHVLLWAGLQRATAAGVTLDLGRTAP
                     EQASLAEFKQRWGAEAVPLAYDYWPNAGGLSQARRDRGPLALAARIWSALPAPVARLG
                     SALYRYLG"
BASE COUNT          371 a          855 c          831 g          300 t
ORIGIN      
        1 ccagtcggcg ccgctcgtgg ccgcgattcc gacgcgcccg tcgggagtca cgtgtgcggc
       61 gcggacctcc cgtcgcgcgc ggcgccagct caccaggcgc tcgagcgtgg cgaaccacgg
      121 tggcgaacca tcggcggaca gggtttggag caagcggtga aacgcctccg gcgcccccgg
      181 aaagcccagc ggcggcgtga gattcgggtg ccacagcccc acccacagcc cgcgcgcggc
      241 ccgggctgcc gctgccagtt cgagtccatc accgatccag gcgcccggat cctcgatccc
      301 ccgatacttg gacaacgccc ggtccatcca taccagggga acgaggtcga gtgagccggt
      361 ggcatcggag tcccaggcgg ggaggatgtc ggctacgccg atgcggaaac cgttgcgatc
      421 gggaaagccg aaggtggcat cgtagctgaa ccccgccgct cgggcgcagg cctgggtcgg
      481 cccgggacgc atacgcagga agtgctgccg cacccctgcg ggcgcactgc cccccagcgc
      541 gcgcccgaga cgcgctcgct cttcccgcat ccgtgtgccg tccagcgcgc tgcggaagct
      601 gccgtgcagg ccgatctcgt gcccatcgcg gcgaatgcgg tcgagcaggc ggcgcgcggc
      661 cgcggattcg agcgagtacg tgatatcccc ggcgagccat gtggcgggcg tgggcgtacc
      721 gcacagcacg aaccacgtcg aatggatgcc cgcggcacgc tcggtgtcca gaatgcgctg
      781 cacgccggcc tgcaccggcc tgcgcccgat tgccgccgcc gcggcaccca gcgagcgggt
      841 gaggcgtccc cattgccctc cacgggccaa ctcgacggcg cgtagcgccg taaacagcgg
      901 ccaccccgac acgatgtcca ggtcgtgcgt gatcgcggcc gcccaccggc gtccctcggg
      961 ccaaggcgtc acgagccgga cggagcgacg ccccgccgcc tgttgaaccg ccgccgcaag
     1021 cgccgccgcc caatgctgca cgacgggctc gcgttcccgg cctgcgcgca ccagcggatt
     1081 ctccgaagac gggacgcggc cgtggcgatc ctcggtcgcg cttccctgct ccacggcggc
     1141 tcccgccagg tccgtaatcc cggagagggc gtgccgtgtg actctcacca ccccgtcgcc
     1201 ccggtccagg ggaaggccgt actgcagcag ggaggcgagg gacgcgggat catcggtgat
     1261 ctcgagccga gtgacgtcgg cttcctcgtc ggccacgagc aggaggcgcg acaagtccac
     1321 caacgcctcg agcccgaatc gttccctggg cgcgaggtcc ggcgggagcg tcaggcggat
     1381 cacgaccagc cgggcggcgg tgtcaggacg cgcaccgtct tcgcgggcag tcccgccact
     1441 acgtgcaacg gcgggacgtc cttcgtgacg acgctgttcg cgccgacgat cgctccgcgc
     1501 ccgatgcgga ctccgggcag caccacgacg cccgcgccga gccaggcgtc ggcctcgatc
     1561 acgatggggg cgcgtttcac gggcgcgaac ggccggatgc gcgagtcgtt gggatgggac
     1621 gagagcacca gcgtgacgcg gggcgcgatg ctcacccggt cgcccagggt caagttaccg
     1681 cgatcttcca gctcctcggc gatcagcagc tcgtcggcga tgtagacgtc gcggccgatg
     1741 gtgtagccgc accagcgcag cagccggatg cgccatcccg ggaaaaacgc gttgcgggcc
     1801 caccgcttga agatcttctt gcgcagccgc cacaggaatc gtcccacggt caccccaggt
     1861 agcgatagag cgccgagccg agacgcgcga cgggcgcggg taaggccgac caaatgcggg
     1921 ccgccagcgc cagggggccg cgatcgcggc gcgcctgcga gaggccgccc gcgttgggcc
     1981 agtaatcata tgccaggggg accgcctccg caccccaacg ctgtttgaac tcggcgaggg
     2041 acgcctgttc cggcgccgta cgcccgaggt cgagcgtcac gccggccgcg gtggcgcgtt
     2101 gcagcccggc ccagagcaga acgtgcgtag gacggtagtc ccgcaccagc gcggggtcgg
     2161 aagcgctcaa ggcgtagatc cagtcgcgcg gtcccttgaa catcacgaag cccgcggcaa
     2221 tgcgtccgtc cgggatgcgg gccagataga gatctacgag ccccgcctcc tgcaggccgc
     2281 ggagcgcatc gaggaagtat cgtcgcggcg gtgccggaac gccgtgtccc tgctgcacgc
     2341 gctcctcgag acgcgcc
//
LOCUS       DBNGZW010000081         7341 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_758317, whole genome shotgun sequence.
ACCESSION   DBNGZW010000081 DBNGZW010000000
VERSION     DBNGZW010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7341)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7341)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7341
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_758317"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="AC16JC_02030"
     CDS             <1..329
                     /locus_tag="AC16JC_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug ABC transporter permease"
                     /protein_id="HIR5163506.1"
                     /translation="LISFTTGLLAFWTTRATAIMELHGGIAMFLAGRIAPLALLPPAI
                     AGLAHVLWFHGMLAFPVEVLTGAAADRTTLLRGFAEQLVWVGLWGGGCWLAWARGVRR
                     YGAVGG"
     gene            347..1195
                     /locus_tag="AC16JC_02035"
     CDS             347..1195
                     /locus_tag="AC16JC_02035"
                     /inference="COORDINATES: protein motif:HMM:NF017946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5163507.1"
                     /translation="MRDRGRVPTGGRGKREGGRVARRWARVLGALVRLNVAEELQYRA
                     NFVASVLGTLFWIGTALLTLALFFRHTERLGGWEYWEVVVLLGVFNALTGVVEAVLRP
                     GIGQLAGEVKSGSLDLVLVRPVDPQLFVSFRQLDLWRVSDIVLGLALAGYALFRLGHT
                     PTLLQSVAFVVALAAAAVVVYAIWVALMSLAFWFVSVENLSVLFDAVYEGARYPVSAY
                     PGALRFVFVYLLPIAWTTTIPAAALTGRLGPAVGILAAAVGAAAFMLSRLLWRAALKR
                     YTGASG"
     gene            complement(1213..1995)
                     /locus_tag="AC16JC_02040"
     CDS             complement(1213..1995)
                     /locus_tag="AC16JC_02040"
                     /inference="COORDINATES: protein motif:HMM:NF025702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HIR5163508.1"
                     /translation="MTRPLSAAALLCAAALAACDKNAVQELPVAPLPGASIKFFNFGV
                     NAPQVNFYANETKMTAVLSGTGQELTTGVAYGSAGNSGLYSAITPGTYTLTGRIAAAT
                     DKDLPIDTVSASLADGKSYSFYLSGFYDTVNKKVDGFVAEDPLPATIDYSVAYVRLVH
                     AISNANALTLYARLTGDTVTAHWAAIGGAVSYKGAGAFMTIVPGVYDLGTRYTDSTTN
                     RITRTGVSLVGGRVYTIGARGDITVVSTTATNRPFLDNTANR"
     gene            complement(2004..3587)
                     /locus_tag="AC16JC_02045"
     CDS             complement(2004..3587)
                     /locus_tag="AC16JC_02045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HIR5163509.1"
                     /translation="MTTRRMALLVSALILTGGCRDWLDVNTNPNGPQSVTANLYLPPM
                     EHWMVTSPQFDGRFVGRYTQEWTLPVSQTSTTPTTWDRMGYDPTSDNGAQQWRDVYWS
                     LGQNLIDMNAKATAEQRWDLLGVGLVLKAWGWQVLTDLHGEIIVKEAFDESRTSFDYD
                     TQDYAYQEVRRLLDSAIVLLRRTDGAVDQAYLGVADHIYGGNRAKWLRFAHGMKALVL
                     NHFSNKATYQPDSVIAEVDSSFTGNGDDALLAYPATSTDFQDYNFWGRSRNNITSYRQ
                     TQFVVNLMNGTQFGGVVDPRMSRMLSPAPDGQYRGLDPNLGGYGGLPAAQQPNNFFGY
                     AGTGGLALPARFIFADKSKLPVMTYAELQFIRAEAALRKGDQATALAAYTNGISASIE
                     FVNARNLDAGQTPTQITATEKSAFLADTNIVPAVGNLTLSHVMCQKFIALWGWGHNEI
                     WMDMRRFHYTDLDPVSGVEVFRGFAAPTNLYPDNGGLVVQRIRPRYNSEYVWNQTGLS
                     AIGALATNYHTKLMWITEP"
     gene            complement(3590..6691)
                     /locus_tag="AC16JC_02050"
     CDS             complement(3590..6691)
                     /locus_tag="AC16JC_02050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018479459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HIR5163510.1"
                     /translation="MKKTVLLVLMSFLLVAEAAFAQQRVVTGRVTSEQGSPLSGVSVS
                     VKGTSVATSTNDDGQYSIRADSGQVLQFRFIGTALVERTVGTEDVIDVALRRVALSLE
                     AVVVTALGQTTTQRALGTSQQAVQGSEIAQTQRENFVNALQGRIAGVDVTSTSGVPGA
                     SSSITIRGVTSISGSNQPLIIVDGLPMDNRTLNTGVLASDQNSNTAFSNRSVDFTNRA
                     ADLNPDDIETLTVLKGPEAAALYGIDAANGAIVITTKRGQSGGGWLYSNSFRMEATRA
                     KPELQRVYGPTSVAPDGTIGSLFYFGTPYAPGTTFYDNVGGFLRTGFTQTHNLSFSGA
                     TPDGKINYRLSTALDRQSGVVPNSDYHRTNVTGHSQAQITPWLNTELSMAYAYDNNNQ
                     VFKGTCGAISTCASSLGPLIGLMLWPQTDNASVYLTPSGLRRRITGLADASAELDNPY
                     YNVNRNKVNATTNRMMVNLGLLITPFSWGSLKTNLGTDNYTNENLIVRDPQSALGVTS
                     NGVLDLANVVTRNIDAQTLLNFNPYNLTSTLSISGTLGHALSDHKSSTDAVVGTGFLD
                     PNFISINNTTNRNPLTTIEQRRLVSVFGKVVLDYRDYLYLTVTGRNDWTSTIPLGHNS
                     FFYPSVSASFVFSDAFPSLRRFVTGKIRAGYAEVGKDARPYAYRPSLQYKTTAYGGYG
                     YNFWGPNLNLRPEFARSLEGGIELGLLHDRLGIDATVYRKVTRDQIVNDIRGSYGTGF
                     ILFNMNGASTRARGLELTVRGTPIVKPAFSWDIVANWTSAGSIVTGLPRSLPESYVSD
                     TWLYGNVRNGDKPGLSTMSLTGTFYRRNNQGELLIDPTTGLPIRSPDFIDAGYDRQPD
                     FTIGITNTLRYKRFTLNFLVDIRKGGDVFDATDHFLTTHGLSMQTLDRDQPRVIKGVL
                     RDGKENTANPTVNTIVVVPSAQTGYYTGMSEELFIERDINWLRLRDVQLSYELPGGFL
                     GSRNASVFVKGTDLLLLTNYTGLDPIVNGNTAAVGGSSGVGIDFGNFPMPAGVNFGLR
                     LGY"
     gene            7057..>7341
                     /locus_tag="AC16JC_02055"
     CDS             7057..>7341
                     /locus_tag="AC16JC_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163511.1"
                     /translation="MKDTGLSRFLSVGTTLFVMVAVGCSLDSPASQLTDPARGGSSAC
                     PKRNALGKFQPADNVAAGFSINGNIVTYSFAAANENPAGGVPGLIKYCVYS"
BASE COUNT         1086 a         2309 c         2595 g         1351 t
ORIGIN      
        1 tcctcatcag cttcacgacg ggcctgctcg ccttctggac cacccgcgcc acggccatca
       61 tggagctgca cggcggcatc gcgatgttcc tcgcgggccg catcgccccg ctggcgctgc
      121 tgccgccggc gatcgcgggg ctggcgcacg tgctctggtt ccacggcatg ctcgccttcc
      181 ctgtggaagt cctgaccggc gcggcggcgg atcggacgac gctgctgcgc ggattcgccg
      241 agcagctcgt ctgggttggg ttgtgggggg gcgggtgttg gctcgcctgg gcgcgcgggg
      301 tacggcgcta tggcgcggtc ggcggatgaa cgacagggga cgggggatga gggacagggg
      361 acgggtcccg acggggggaa gggggaagcg ggaaggggga agggttgccc ggcgatgggc
      421 ccgcgtcctc ggtgccctcg tccgcctcaa tgtcgccgag gagctgcagt accgcgccaa
      481 tttcgtcgcg agcgtgctgg ggacgctgtt ctggatcgga accgccctgc tcacgctggc
      541 gctgttcttc cggcacaccg agcgcctggg gggctgggag tactgggaag tggtggtgct
      601 gctcggggtg ttcaacgcgc tgacgggcgt ggtcgaagcg gtgctgcgcc cggggatcgg
      661 ccagctggcg ggtgaagtga agagcggctc gctcgacctc gtgctggtgc gcccggtgga
      721 cccacaactg ttcgtgtcgt tccgccagct cgacctgtgg cgcgtgtcgg acatcgtgct
      781 ggggctggcg ctcgcgggct acgcgctgtt ccggctgggc cacacgccga cgctgctgca
      841 gtcggtcgcc ttcgtcgtcg cgctcgccgc ggcggcggtg gtggtctacg cgatctgggt
      901 ggcgctgatg agtctcgcct tctggttcgt ctcggtcgag aatctctcgg tgctgttcga
      961 tgcggtgtac gagggggcgc gctaccccgt ctcggcgtat cccggcgcgt tacgcttcgt
     1021 cttcgtctac ctgctgccga tcgcctggac caccacgatc ccggccgcgg cgctcaccgg
     1081 ccgcctcggc cctgcggtgg ggatcctggc cgcagccgtc ggtgcggcgg cgttcatgct
     1141 ctcccgcctg ctgtggcggg cggcgctcaa gcgctacacg ggcgcgagcg gctgagcgac
     1201 ggcgctcggc gcctagcggt tggccgtatt gtccaggaat ggccggttgg tcgccgtcgt
     1261 ggacacgacc gtgatgtcgc cccgtgcacc gatggtgtag acccgccccc ccacgagcga
     1321 cacgccggtt cgggtgatcc tgttcgtcgt cgaatcggtg tagcgggtgc ccaggtcgta
     1381 cacgccggga acgatggtca tgaacgcgcc cgctcccttg tacgacaccg cgccgccgat
     1441 ggcggcccag tgggcggtca ctgtgtctcc cgtgagccgg gcgtacagcg tcagcgcatt
     1501 cgcgttgctg atcgcgtgga ccaggcggac ataggcgacc gagtagtcga tcgtagccgg
     1561 cagcgggtcc tccgccacga acccgtccac cttcttgttc accgtatcgt agaagccgct
     1621 caaatagaac gagtaggatt tcccgtcggc gagcgaggcg ctgacggtgt cgatggggag
     1681 atccttgtca gttgccgccg cgatccgtcc agtgagggtg taggtgccgg gtgtgatggc
     1741 cgaatagagg ccgctgttcc ccgcgctgcc gtaggccaca ccggtggtca attcctgccc
     1801 cgtgccggag aggacggccg tcatcttggt ctcgttggcg tagaagttca cttgcggcgc
     1861 gttcacgccg aagttgaaga acttgatgct cgccccgggc aggggcgcca ccggaagttc
     1921 ctgcaccgca ttcttgtcgc acgcggcgag cgcggccgcg cacagcagcg ccgcggcgga
     1981 gaggggtcga gtcatggtcg ccttcacggt tcggtgatcc acatcagctt ggtgtgataa
     2041 ttcgtcgcca gggcgccgat ggcggacagc cccgtctgat tccaaacgta ctccgagttg
     2101 tagcgcggcc ggatgcgctg gacgaccagg ccgccgttgt cggggtagag gttggtcggc
     2161 gctgcgaaac cgcggaatac ctcgacgccg ctcacggggt cgagatcggt gtagtgaaag
     2221 cgacgcatgt ccatccagat ttcgttgtgt ccccagcccc acagcgcgat gaatttctgg
     2281 cacatgacgt gcgacagggt caggttgccg accgccggca cgatgttggt gtccgcgaga
     2341 aaggcgctct tctccgtcgc cgtgatctgc gtcggcgtct ggccggcgtc gaggttcctg
     2401 gcgttcacga actcgatcga agccgagatc ccgttggtgt acgccgccag cgccgtggcc
     2461 tggtctcctt tgcgcagcgc ggcctcggcc ctgatgaact gcagctccgc gtaggtcatc
     2521 accgggagct tggacttgtc ggcgaagatg aaccgggcgg gcagggccaa ccccccggtg
     2581 ccggcgtacc cgaagaagtt gttcggctgc tgcgcggcgg ggagtccccc gtacccgccg
     2641 aggttcggat ccagcccgcg gtactgcccg tcgggagcgg gcgagagcat gcggctcatc
     2701 cgcgggtcca ccacgccgcc gaactgcgtg ccgttcatca ggttcacgac gaactgggtc
     2761 tgccggtaac tggtgatgtt gttgcggctg cggccccaga agttgtagtc ctggaagtcg
     2821 gtgctggtgg cgggataggc gagcagcgcg tcgtcgccgt tgcccgtgaa cgacgaatcc
     2881 acttccgcga tcaccgaatc cggctgatac gtcgccttgt tggagaaatg gttcagcacc
     2941 agggccttca tgccgtgggc gaaccggagc cacttggccc ggttcccgcc gtagatgtga
     3001 tccgccacgc cgagataggc ctggtccacg gcgccgtcgg tgcgccgcag cagcacgatc
     3061 gcgctgtcga gcagccgccg gacttcctga taggcgtagt cctgcgtgtc gtagtcgaac
     3121 gacgtgcgcg actcgtcgaa ggcttccttg acgatgatct cgccgtggag gtcggtgagc
     3181 acctgccagc cccacgcctt gagcacgagc ccgaccccca gcaggtccca gcgctgctcg
     3241 gcggtcgctt tggcgttcat gtcgatcagg ttctggccca gcgaccaata gacgtcccgc
     3301 cactgctggg caccgttgtc gctggtcgga tcgtatccca tccggtccca ggtggtgggc
     3361 gtggtgctgg tctggctgac cggcaaggtc cactcctggg tgtagcggcc gacgaagcgg
     3421 ccgtcaaact ggggcgacgt caccatccag tgctccatcg gcggcaggta cagattcgcc
     3481 gtcaccgact gcgggccgtt cggattggtg ttgacgtcca gccagtcccg gcacccgccg
     3541 gtcaggatca gcgcgctcac caggagcgcc atgcgtcgcg tggtcatggt cagtatccca
     3601 gtcgcaggcc gaagttgacg ccggcgggca tggggaagtt gccgaagtcg atccccaccc
     3661 cgctcgatcc gcccactgcg gcggtgttgc cgttcacgat cgggtcgagc ccggtgtagt
     3721 tggtgagcag cagaaggtcc gtgcccttga cgaacacgct ggcgttgcgg gagccgagga
     3781 agccccccgg cagctcgtag ctcagctgca cgtcgcgcag gcgcagccag ttgatgtcgc
     3841 gctcgatgaa cagctcttcg ctcatgccgg tgtagtagcc cgtctgcgcg ctcggcacga
     3901 cgacgatggt attgacggtg ggattggcgg tgttctcctt gccgtcgcgc aacacgccct
     3961 tgatcacccg cggctggtcg cggtcgagcg tctgcatgct gaggccgtgc gtcgtgagga
     4021 aatggtccgt cgcgtcgaag acgtcgccgc ccttgcggat gtccaccagg aaattgaggg
     4081 tgaagcgctt gtagcggagc gtgttggtga tgccgatcgt gaaatcgggc tggcggtcgt
     4141 acccggcgtc gatgaagtcc ggcgagcgga tcggcaggcc ggtggtgggg tcgatcagca
     4201 gctcgccctg gttgttccgc cggtagaacg tccccgtaag cgacatggtg gagaggcccg
     4261 gcttgtcgcc gttgcgcacg ttgccgtaga gccaggtgtc ggacacgtac gactcgggca
     4321 gcgagcgggg caggccggtc acgatcgacc ccgcgctcgt ccagttggcc acgatgtccc
     4381 aggagaacgc gggtttcacg atcggcgtgc cgcgcaccgt cagctccagc ccgcgggcgc
     4441 gcgtggaagc gccgttcatg ttgaagagaa tgaacccggt gccgtaggaa ccgcggatgt
     4501 cgttgacgat ctggtcgcgg gtgaccttgc ggtacacggt cgcgtctatg ccgaggcggt
     4561 cgtgcagcag gcccagctcg atgcccccct cgagggaccg ggcgaactcg ggacgcaggt
     4621 tcaggttcgg accccagaag ttgtacccgt agccgccgta cgccgtcgtc ttgtactgga
     4681 gcgacggccg gtacgcgtac ggccgcgcgt ccttgccgac ctcggcgtag cccgcccgga
     4741 tcttgccggt cacgaagcga cgcagcgacg ggaaggcgtc ggagaacacg aagctggccg
     4801 agacggacgg atagaaaaag gagttgtgcc cgagcggaat ggtcgaggtc cagtcgttgc
     4861 ggcccgtgac cgtcaggtac aggtaatcgc ggtaatccag caccactttt ccgaacacgc
     4921 tcaccaggcg gcgctgctcg atcgtcgtca gcggattccg gttggtcgtg ttattgatcg
     4981 aaatgaagtt cggatcgagg aacccggtgc cgaccaccgc gtcggtcgag ctcttgtggt
     5041 cggagagggc gtgccccagc gtgccgctga tggagagggt ggaggtcagg ttgtacgggt
     5101 tgaaattcag cagcgtctgc gcgtcgatgt tccgcgtgac gacgttcgcc aggtcgagca
     5161 cgccgttcga cgtcacgccg agcgcgctct ggggatcgcg gacgatcagg ttctcgttgg
     5221 tgtagttgtc ggtgccgagg ttggtcttga ggctgcccca cgagaacggc gtgatcagga
     5281 ggccaaggtt caccatcatg cggttggtgg tggcgttgac cttgttcctg ttgacgttgt
     5341 aatacgggtt gtccagctcc gcggaggcat cggccaggcc cgtgatgcgg cgccgcaggc
     5401 cggagggcgt gagatagacg ctcgcgttgt cggtctgggg ccacagcatc aggccgatga
     5461 ggggccccag cgagctggcg cacgtgctga tcgcgccgca cgtgcccttg aacacctgat
     5521 tgttgttgtc gtaggcgtag gccatggaca gctcggtgtt cagccagggt gtgatctgcg
     5581 cctgggagtg ccccgtcacg ttggtgcggt ggtagtcgct gttcggcacc acgcccgact
     5641 gccggtccag cgctgtggac agccggtagt tgatcttccc gtccggcgtc gccccgctga
     5701 acgacaggtt gtgggtctgc gtgaatccgg tgcgcaggaa gccgccgacg ttgtcgtaga
     5761 acgtggtgcc cggggcgtac ggcgtgccga agtagaagag cgagccgatc gttccgtccg
     5821 gcgccacgct ggtggggccg tagacgcgct gcaactcggg cttggcgcgc gtcgcctcca
     5881 tgcggaagct gttgctgtac agccagccgc cgcccgactg cccccgcttg gtcgtgatga
     5941 cgatggcgcc gttcgccgca tcgatgccgt acagcgccgc cgcctccggt cccttcagca
     6001 ccgtcagggt ctcgatgtcg tcgggattga gatcggccgc gcggttggtg aagtccacgc
     6061 tgcggttgga gaaggcggtg ttggagttct ggtcggaggc gagcacgccg gtgttgagcg
     6121 tccgattgtc catcggcagc ccgtccacga tgatcagcgg ctggttgctg ccgctgatgg
     6181 acgtcacgcc gcggatggtg atggacgacg acgcccccgg cacccccgac gtactcgtca
     6241 catccacgcc ggcgatgcgg ccctgcagcg cgttgacgaa attctcccgc tgcgtctgcg
     6301 cgatctcgct gccctgcacc gcctgctgcg aggttcccag ggcccgctgc gtggtggtct
     6361 ggcccagcgc cgtcaccacc accgcttcga gactgagggc cacgcgccgc agcgccacgt
     6421 cgatcacgtc ttcggtgccg acggtccgct ccaccagcgc ggtcccgatg aaccggaact
     6481 gcagcacttg gccgctgtcg gcccggatgg agtactggcc gtcgtcgttg gtcgaggtcg
     6541 caacgctcgt ccccttgacg ctcaccgata ccccgctcag aggcgagcct tgctcgctcg
     6601 tgaccctgcc cgtgacgacg cgctgctgtg cgaacgccgc ctcggcaacg aggaggaaac
     6661 tcatcaacac caggagaacg gtctttttca tggtgacggc catggggttg ggtagacagc
     6721 agtcaggctc ggatgccccg cccgaacggg atgggaactg acattaccgc ccggtccggg
     6781 agaccgcaag cagggttccg ctcgtcgcgg cgcttcagac gactgtcagg cgccctcccg
     6841 cgcccggtgc gcggccgcca cgccagggtg caccgcccac ctgcgcggtc gtggcgtcgt
     6901 ggccacgccg ggcgtcaagg ttgccgggcg gcggaccata ccaccgcgac gtttcaccga
     6961 ttctgatcgg gcattgcgct tgcagcagat gggcgggggt gaacaggggg cgcgaggtgt
     7021 tgctccgacg cgacggctca aaaagaggag tgccaaatga aggataccgg gctctcgagg
     7081 ttcctctcgg tcggcacgac gctgttcgtg atggtggcgg tcggatgttc cctcgactcg
     7141 cctgccagtc aactgaccga tcccgcgcga ggtgggagca gcgcctgccc gaagcgcaac
     7201 gcgttgggaa aattccagcc ggccgacaat gtggcggccg gcttctcgat caacggcaac
     7261 atcgtcacct actcgttcgc ggcggcgaac gagaatccgg ccgggggagt acccggcctg
     7321 atcaaatact gcgtctattc g
//
LOCUS       DBNGZW010000082         7872 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1709363, whole genome shotgun sequence.
ACCESSION   DBNGZW010000082 DBNGZW010000000
VERSION     DBNGZW010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7872
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1709363"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            9..752
                     /gene="lptB"
                     /locus_tag="AC16JC_02060"
     CDS             9..752
                     /gene="lptB"
                     /locus_tag="AC16JC_02060"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HIR5163512.1"
                     /translation="MESRGLVKAYKGRRVVNDVSIQLQQGEIVGLLGPNGAGKTTTFY
                     MFTGLIRPEQGTILLDGHDITAEPMYRRARAGLGYLAQEPSVFRKLTVEQNVLAILET
                     LPIDAAERGVRLERLLDELGVKHLRRQKAFQLSGGERRRLEITRALVTEPKFLLLDEP
                     FAGVDPIAVHDIQTIVAGLRQRGIGVLITDHNVEQTLDIVDRAYIMFEGKVQVSGTVR
                     ELVFNDDVADRYLGPTLTARLRTRLGQAA"
     gene            749..2215
                     /gene="rpoN"
                     /locus_tag="AC16JC_02065"
     CDS             749..2215
                     /gene="rpoN"
                     /locus_tag="AC16JC_02065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HIR5163513.1"
                     /translation="MKPGLQQHTGMRQDLRINPRLFQAMDLLYMPLLDLQQHLKQELL
                     GNPFLEMVETEEEPEEAQSAAAEKEKIEKDKEAEDGPNWEDILLDDGAEAMAGRRDLS
                     EALEYTEPVSVEMRDLSDHLKDQVQMLDLTPRQQLLAEEFLGNIAEDGYLGATLEQII
                     DGANKLLADFAEQRNGDGEVALYTLSEAEEMLGIIQRLDPPGVGARDLRECLLLQLED
                     KKQTDTLAYRLVKEAWEDLKNHRWSDLGKRFGIEAGEVQRVADVLAKLDPKPGLQYAA
                     ANDTYIIPDLTVEKVGDSYHVTLNDGGLPRLRISKTYQDIARDRKKFQGENKDFINQR
                     LNSAHWMIQAIEQRRQTMLKVMNFIVDRQREFFEKGVEYLRPLTLREVADVINMHEST
                     VSRVTNEKYVQTPRGLLPLKFFFSSGLQTASGEDASARSIKAQIQKMVGDEDPRNPLT
                     DQQIVEMFGNKGVRIARRTVAKYRDQLNILPARMRRRL"
     gene            2212..3180
                     /locus_tag="AC16JC_02070"
     CDS             2212..3180
                     /locus_tag="AC16JC_02070"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5163514.1"
                     /translation="MTLAAGNGEAAARPDVSVLIPAKDEAENLNELVRLLQDALAPQP
                     YRCEVVVIDDGSRDATPEVLRRLAAQHEFLRVVTHRAQRGIADALRSGAEAAAGRVLV
                     FWPADLQYLPADIPALVAPVLADEADIATGAKQGHYDKRFVSAVYNRLCRWLFGVRVT
                     DLNSVKAYRREVMDGLPMRPDWHRFMVVIAAGQGFRLTERPVPLHPRRAGRSKFGIGR
                     IPVGVLDLISVWFQLRFGRKPMLFFGVTGALLVFLGIVVGVWEFVQRYVHQQGNRAVL
                     YLVMLLVLAGLILFAFGFVGELVAGQREEIRALHREIDQMREEQSS"
     gene            complement(3356..4783)
                     /locus_tag="AC16JC_02075"
     CDS             complement(3356..4783)
                     /locus_tag="AC16JC_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR5163515.1"
                     /translation="MAPLSLAARLYAALPWVSQRVRAILTSLAASNGSVGSADHLARI
                     AALRSRFQLARLLRRDGLPPVGELVAWTRVLYWLHQAEVDGSTLGHLVLVDGIDPATA
                     SRLVKRVLGITWTTARTRGFHSAFDRFVTECHRRHVARLARGRDAVVSRPGTRLRVGG
                     KPGCCSGCAPGQPEEPRAREIGARVPLAGQPFDVAVSRHGAVYVTCGHAAVVQRLDLE
                     AASPGRSIAVGSVPSCVTFSADGLRAYVTNQFSRTVGIIEVGRHRQIDEIPLPGDPVP
                     LLLDPDQNTVHVTTNQDALLAVDLTSHRVTRRLPLPATSHHLALHPDGRRLYVATRAA
                     GTVLEVDPEDYRVLRQFDLGGMPQGLVIVRSGARLLVANEGGMIQSVDLKAGRVAEQS
                     DLGGGAFGLAVSPGEDVLYAGLPASGQIRVLDVETLAHRGVIEVGGVPRSLAFDLHGR
                     WLIAVNEGGWVDLVRPVRGKGSAAA"
     gene            4894..6102
                     /locus_tag="AC16JC_02080"
     CDS             4894..6102
                     /locus_tag="AC16JC_02080"
                     /inference="COORDINATES: protein motif:HMM:NF014427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR5163516.1"
                     /translation="MRNVRQIAWLFLAVAGSACGNSTGPSGPKVAQFRVTPDTIRLVR
                     GDSVALTVTPLDANGNLLVGVAVAVSFTSADTGIAKVSEIGLVKSGGRLGDTKITVTG
                     GGVSQDVPASVLGIFGHTILAGSPFGTAATRLGASYVLRHTLNLVSRFNLPDTAVATS
                     FGIADNPTYVTFDSAGTTAYATAQFAQRVLVINAVTNTIVDSIPTTGDPDIVRVSPDG
                     HSIWVSTNVDSLYQFDRASKTILARYGLPLIPNGIAFAPTNDSLLYVSTLWAGAVVEI
                     NYKRKTFGRTFTPGGVTQAVVISPDGKELYVSNESLGEIEIYNLASGVALPVIKTDGP
                     AFDLELSPDGRLLWTSLSGAGVVRAYDRTSRALRYTFVTGGVPRRIAMTPATTEVVVA
                     NEAGWVDFIK"
     gene            6175..7362
                     /locus_tag="AC16JC_02085"
     CDS             6175..7362
                     /locus_tag="AC16JC_02085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5163517.1"
                     /translation="MRLLVLTHNYPRFSGDPAGAYVARLARAAADAGAEVQVVAPHTG
                     NTPEREIAERFSLHRFRYAPEGLEQVGYRGDSGRGLLHQPLTLALAPFYLGRFRRAAR
                     RAVAELSPSVVHAHWWFPGGWVAASLGPPYVVTSHGSDVRLFDRSGLWRRLGHRVLAG
                     AGAVTAASRFLAEDVERHAGPLPQPVAVSPMPVDADLFMVGRSVPKATPPRILYAGNL
                     VPSKGVDVLLHALASLRAQGIACRGKILGAGPAQPALQRLARELGLSADMEWSPFVPQ
                     QRMPAEYGASTVTVLPTRGQAEGLGLTLVEALLSGSAVVGTPAGGIPEVVQDNETGLL
                     ARDGDAQDLAAKIARLLQDPALRDRLTQAGQARVLDRYSPQAAARRFLALYDAIASRH
                     ATR"
     gene            7331..>7872
                     /locus_tag="AC16JC_02090"
     CDS             7331..>7872
                     /locus_tag="AC16JC_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163518.1"
                     /translation="MPLLAVTRHAEPPPGWDGLVAQYGTFYHDRRWLLALSRCFDYRL
                     HVLTVGPDSALTGGLALAETPAMLGRGRLVSFPFSFVAGPLATDADTRASLATAARDL
                     AVELRVRRLELKQLGIHHPPPEGFERSTRYSTYRVTTQGGEQAVWKRLHVTSTQQRIR
                     KGQKAGVTVVDGRSADDWLA"
BASE COUNT         1310 a         2763 c         2587 g         1212 t
ORIGIN      
        1 gcagccgcat cgagagccgt ggcctggtca aggcctacaa gggccggcgc gtggtcaacg
       61 acgtgagcat ccagctgcag cagggggaga tcgtcggcct gctgggcccc aacggggcgg
      121 gcaagaccac gacgttctac atgttcacgg ggctgatccg gcccgagcag ggcacgatcc
      181 tcctcgacgg acacgacatc acggccgagc cgatgtaccg ccgcgcgcgc gcggggctgg
      241 gctatctcgc ccaggagccc tccgtcttcc gcaagctcac cgtggagcag aacgtgctcg
      301 ccatcctcga gacgctgccg atcgacgccg ccgagcgggg cgtccggctc gagcgcctgc
      361 tcgacgagct gggcgtgaag cacctgcggc gccagaaggc ctttcagctc tccggcggcg
      421 agcggcgccg tctcgagatc acgcgcgcac tcgtcacgga gccgaagttc ctgttgctcg
      481 acgagccgtt cgccggggtg gatcccatcg ccgtgcacga catccagacg atcgtggcgg
      541 gcctacggca gcgcggcatc ggcgtgctca tcaccgatca caacgtggag cagacgctcg
      601 acatcgtgga tcgcgcctac atcatgttcg aagggaaagt gcaggtgtcg gggaccgtgc
      661 gcgagctggt gttcaacgac gacgtggccg accgctatct cggccccacc cttacggccc
      721 ggctccgtac ccggctgggg caagcggcat gaaacccggg ctgcagcagc acaccgggat
      781 gcgccaggac ctgcgcatca acccgcgcct gttccaggcc atggacctgc tgtacatgcc
      841 gctgctcgac ctgcaacagc acctgaagca ggagctgctc ggcaacccgt tcctcgagat
      901 ggtggagacc gaggaggagc ccgaagaagc ccagagcgcg gccgccgaga aggagaagat
      961 cgagaaggac aaggaagccg aggacgggcc gaactgggaa gacatcctgc tcgacgacgg
     1021 cgccgaagcc atggcgggac gccgcgatct ctccgaggcg ctcgagtaca ccgagccggt
     1081 ctcggtcgag atgcgcgatc tctccgacca cctgaaggat caggtccaga tgctggatct
     1141 gacgccgcgc cagcagctgc tcgccgagga gttcctcggc aacatcgcgg aagacggcta
     1201 cctgggcgcc acgctggagc agatcatcga cggcgccaac aagctgctcg cggacttcgc
     1261 cgagcagcgc aacggggacg gcgaggtcgc gttgtacacg ctgagcgaag ccgaagagat
     1321 gctgggcatc atccagcgcc tcgatccgcc aggcgtgggc gcccgcgacc tgcgcgagtg
     1381 cctgctgctc cagctcgagg acaagaagca gaccgatacg ctcgcctacc ggctggtcaa
     1441 ggaggcatgg gaagacctga agaaccaccg ctggagcgac ctgggcaagc gcttcggcat
     1501 cgaggcgggc gaagtgcagc gcgtcgccga cgtgctggcg aagctcgacc cgaaacccgg
     1561 cctgcagtac gcggcggcca acgacaccta tatcattccc gatctgaccg tcgagaaggt
     1621 cggcgacagc tatcatgtga cgctcaacga cggcggactg ccgcggctcc gcatctccaa
     1681 gacctatcag gacatcgccc gcgaccgcaa gaaattccag ggcgagaaca aggacttcat
     1741 caaccagcgg ctcaacagcg cccactggat gatccaggcg atcgagcagc ggcgccagac
     1801 catgctcaag gtgatgaact tcatcgtcga tcggcagcgg gagttcttcg agaagggggt
     1861 cgagtacctc cggcccctca cgctgcgcga ggtggcggac gtcatcaaca tgcacgagag
     1921 cacagtcagc cgggtgacca acgagaagta cgtccagacg ccgcgcgggc tgctgccgct
     1981 caagttcttc ttctcgagcg gcctccagac cgcctccggc gaggacgcca gcgcgcggtc
     2041 gatcaaggcg cagattcaga agatggtggg cgacgaggat ccgaggaacc cgctgaccga
     2101 ccagcagatc gtggagatgt tcggcaacaa gggcgtgcgc atcgcgcgcc gcaccgtggc
     2161 caagtaccgc gaccagctca acattctccc cgcccgcatg cggcggcggc tgtgaccctg
     2221 gccgcgggaa acggggaagc ggcggcccgg cccgacgtga gcgtcctcat tcccgccaag
     2281 gacgaggcgg agaacctcaa cgagctggtg cggctgctgc aggacgcgct cgcgccgcag
     2341 ccgtaccgct gcgaggtcgt cgtgatcgac gacggcagcc gcgacgccac ccccgaggtc
     2401 ctgcgccggc tcgccgccca gcacgaattc ctgcgggtcg tcacccatcg cgcccagcgc
     2461 ggcatcgccg acgcgctgcg cagcggcgcc gaggccgcgg cggggcgcgt cctcgtgttc
     2521 tggcccgccg atctccagta tctgcccgcc gacatccccg cgctcgtggc gcccgtgctg
     2581 gccgacgagg ccgacatcgc cacgggggcg aagcaggggc attacgataa gcggttcgtc
     2641 tccgccgtct acaaccggct gtgccgctgg ctcttcgggg tgcgggtgac cgacctcaac
     2701 tcggtgaagg cgtatcgccg cgaggtcatg gacggactgc cgatgcgccc cgattggcat
     2761 cgcttcatgg tcgtcatcgc ggcggggcag gggttccgcc tcaccgagcg cccggtgccg
     2821 ctgcacccgc ggcgcgcggg ccggtccaag ttcggcatcg gccggatccc cgtgggcgtg
     2881 ctcgatctca tttcggtgtg gttccagctg cggttcggac gcaagccgat gctgttcttc
     2941 ggcgtgaccg gggcgctgct cgtgtttctg ggcatcgtcg tgggggtgtg ggaattcgtc
     3001 cagcgctacg tccatcagca gggaaaccgg gcggtcctgt acctggtgat gctgctcgtg
     3061 ctcgccgggc tgattctgtt cgcgttcggg ttcgtgggag agctcgtggc ggggcaacgg
     3121 gaggagatcc gggcgctgca ccgcgagatc gaccagatgc gggaggagca atcgtcttga
     3181 cggaccgaac gcagcggcat cggtctctac cacccagccc gatcgccctg ctcgccgtcg
     3241 ccgtgctggc ctgcggtccc tccgaaccat cgagctgcac accgcactgc tgaacgaagt
     3301 gggcgatacg ctggcgaacg attcggggtg ggtcgacgtc atcaagccgt gaccgtcagg
     3361 cggccgccga acccttgcct cgaaccggcc gtacgaggtc gacccagccc ccttcgttga
     3421 cggcgatcaa ccaccggccg tggaggtcga acgccaggct gcggggaacg ccgcccacct
     3481 ctatcacccc gcgatgcgcc agcgtctcca cgtcgagcac ccggatctgc cctgaagccg
     3541 gcagcccggc gtacagcaca tcctcccccg gcgacacggc cagcccgaac gctccacccc
     3601 ccaggtccga ctgctcggcc acgcgcccgg ccttcaggtc gaccgattgg atcatgcctc
     3661 cctcgttcgc cacgaggagc cgtgcgccgc tccgcacgat gaccagcccc tggggcatgc
     3721 cccccaggtc gaactgccgc aacaccctgt agtcctcggg gtccacctcg aggaccgttc
     3781 ccgcagctcg cgtggccacg tacagccgcc tgccgtccgg atgcaacgca aggtggtgcg
     3841 acgtcgcagg aagcgggagg cgccgagtca cgcgatgcga ggtgaggtct accgcaagca
     3901 gggcatcctg atttgtcgtg acatgtacgg tgttctgatc cggatcgagc agcaacggga
     3961 ccgggtcacc cggaaggggg atctcgtcta tctggcggtg tcgccccacc tcgatgattc
     4021 ccaccgttct gctgaactga ttcgtcacat aggcacgcag gccgtcggcg ctgaacgtca
     4081 cgcagctggg gacgctcccg acggctatcg accgccccgg cgaggctgcc tccagatcga
     4141 gccgctgcac gacggcggcg tggccgcagg tcacgtagac tgcgccgtga cgcgaaaccg
     4201 cgacgtcgaa cggctgtccg gcgagcggca ctcgcgcccc gatctcgcgc gcacgtggtt
     4261 cctcgggttg cccgggtgcg cacccgctgc aacacccggg tttcccgccc acgcgcaggc
     4321 gagtgcccgg ccgcgacacg acggcatccc ggccccgcgc gaggcgcgcg acgtgccggc
     4381 ggtgacattc cgttacgaag cgatcgaagg ccgagtgaaa tccgcgcgtg cgcgccgtgg
     4441 tccaggtgat ccccaggacg cgtttcacca ggcgagacgc cgtggccgga tcgatgccat
     4501 ctaccaggac caggtgcccc agggtcgagc cgtcgacctc ggcttgatgc agccagtaca
     4561 gtacgcgcgt ccaggcgacc agctcaccca ccggcggcag accgtcacga cggagcaggc
     4621 gagccagctg gaagcggctg cgcagtgcgg caatccgtgc caggtggtcc gccgaaccga
     4681 cgctcccatt ggaggcggcg agtgaggtca gaatcgccct gactcgttgt gagacccagg
     4741 ggagcgccgc gtagagcctg gccgctaacg acaatggcgc catgatgtta cgtcgttgcc
     4801 gcacaatcgg tcatggcgct cttcccctgc aggaatcatg ctttggatgg cccttttcgc
     4861 cggctcagcc tcgagccgca ttcgggagat accatgcgaa acgtcaggca aattgcatgg
     4921 ctgtttctgg cggtcgccgg aagcgcatgc gggaacagca caggaccgtc gggccccaag
     4981 gtcgcacagt tccgcgtcac tcccgataca atccggctcg tccgcggaga cagcgtggcc
     5041 ctcacggtga ctcccctgga tgccaacggc aatctcctgg tgggagtcgc cgtcgccgtt
     5101 tcgttcacat ccgcggatac cgggatcgcc aaggtgtcgg agataggtct ggtcaagtcc
     5161 ggggggcggc tgggagacac caagatcacc gttacgggcg gcggggtcag tcaagacgtc
     5221 ccggcttcgg tcctgggcat tttcgggcac accatcctcg ctggtagtcc gttcggcact
     5281 gcggccacac ggctgggcgc ctcctacgtg ctgcggcaca ccctcaatct cgtgtcccgc
     5341 ttcaacctcc ccgacacggc ggtcgccacg tcgttcggga ttgccgacaa ccccacgtac
     5401 gtcacgttcg attccgccgg gaccaccgcc tacgccacgg cgcagttcgc ccagcgtgtc
     5461 ctggtcatca atgccgtcac gaataccatc gtggattcga tccccacgac cggggacccg
     5521 gacatcgtac gcgtgtcgcc cgacggccat tcgatctggg tgtccaccaa cgtcgactcg
     5581 ctctaccagt tcgatcgcgc gagcaagacc atcctggcgc gctacggtct tcccctcatt
     5641 ccgaacggca tcgccttcgc ccccacgaac gattcgctgt tgtacgtcag cacactgtgg
     5701 gccggcgcgg tggtcgaaat caactacaaa cgcaagacgt tcgggcgcac gttcacaccg
     5761 ggaggcgtca cccaggccgt cgtcatctcg cccgacggca aggagctgta tgtctcgaat
     5821 gagtcactgg gcgagatcga gatctacaac ctggcatccg gtgtggcgct gcccgtcatc
     5881 aagaccgacg gtccagcctt cgacctcgaa ttgagccccg atggccggct gctgtggacc
     5941 tcgctgtccg gtgccggcgt ggtccgcgcc tacgatcgga cttccagagc cctccgctac
     6001 accttcgtca ccggcggagt accgcgccgc atcgcgatga ccccggcgac caccgaggtg
     6061 gtcgtggcca acgaagcggg gtgggtcgac ttcatcaagt agcccgctcg cctcaccggc
     6121 ccctccccga cagcgccccg agtcgacggg cgctgtcgct tatattcagg gccgatgcgc
     6181 ttgctggtgc tcacgcacaa ttacccccgg ttctcgggcg acccggcggg cgcgtacgtc
     6241 gcccgcctgg cgcgagcggc cgccgacgca ggcgccgagg tccaggtcgt cgcgccccat
     6301 acggggaata cacccgagcg ggagatcgcg gagcggttct cactccatcg cttccggtac
     6361 gccccggagg gactcgagca ggtcgggtat cgcggggaca gcggacgagg actgctgcac
     6421 cagccgctca ccctcgcact ggcgccgttc tacctgggtc gctttcggcg agccgcgcgc
     6481 cgggccgtgg ccgagctgtc gccgtcggtc gtgcatgccc actggtggtt ccccggcggg
     6541 tgggttgcag ccagcctggg gccgccgtac gtcgtcacgt cgcacgggag cgacgtccgg
     6601 ctcttcgacc gcagtggtct gtggcgccgt ctcggacacc gtgtgctcgc cggggcaggg
     6661 gcggtgaccg ccgcctcgcg ctttctcgct gaagacgtcg agcgtcacgc cggtccgctg
     6721 ccgcaacccg tcgccgtcag cccgatgccc gtggacgccg acctctttat ggtcgggcgg
     6781 tccgttccca aggccacccc tccgcgcatc ctgtacgccg gcaatctggt tccatccaag
     6841 ggggtggacg tcctgctgca cgcactggcg tcgctgcgcg ctcagggcat cgcctgccgc
     6901 ggcaagatcc tcggtgccgg cccagcccag cccgctctgc agcggctcgc cagggagctg
     6961 gggctgagcg cggacatgga gtggtctccc ttcgtgccgc agcagcgtat gcccgccgaa
     7021 tacggagcca gcacggtcac cgtgctgccg acccgtggcc aagcggaggg actcgggctc
     7081 accctggtcg aagccctcct gtcgggatcc gcggtcgtcg ggactcccgc cggcggcatc
     7141 ccggaagtcg tccaggacaa cgagaccggc ctgctcgcca gggacggcga cgcacaggac
     7201 ctcgcggcaa agatcgctcg cctgcttcag gacccagcgc tgcgcgaccg actcacgcag
     7261 gcgggacagg cgcgcgtcct cgaccgatac tccccgcagg ctgccgcacg ccgctttctc
     7321 gctctctacg atgccattgc tagccgtcac gcgacacgct gagcccccgc ccgggtggga
     7381 cgggctcgtc gcgcagtacg gcacgttcta tcacgaccgg cgctggctgc tcgcactttc
     7441 ccgctgcttc gattaccggc tgcacgtcct gacggtggga ccggactccg cgctcacggg
     7501 cggcctggcc ctggccgaaa cgccagccat gctcggccgc ggccggctgg tgagctttcc
     7561 tttcagcttc gttgccggac cgctcgcgac cgatgccgac acgcgtgcgt cgctcgccac
     7621 ggcggcacgc gacctcgccg tcgagctgcg ggttcgccgc ctcgagctga aacagctcgg
     7681 gatccaccat ccgccacccg aaggattcga gcgcagcacc cgctactcga cctatcgcgt
     7741 gacgacgcag ggtggggaac aggccgtctg gaagcggctg cacgtcacga gcacgcagca
     7801 acgcatccgc aaaggtcaga aagccggtgt cacggtcgtc gacggccgct ccgcggacga
     7861 ctggctcgcg at
//
LOCUS       DBNGZW010000083         4776 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1576412, whole genome shotgun sequence.
ACCESSION   DBNGZW010000083 DBNGZW010000000
VERSION     DBNGZW010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1576412"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..2690
                     /locus_tag="AC16JC_02095"
     CDS             84..2690
                     /locus_tag="AC16JC_02095"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HIR5163519.1"
                     /translation="MTTISCFLLCLAAAAPVPSVTVDSPYIVAPQVRAMRAVQPLVVD
                     GRLDDPVWRTAERVSGFLQRDPLEGKAASESTVVYVAYDDAAIYIGARMYDAHPDSIV
                     GRLGRRDDYANSDRFNVFLDPYHDRRSGVYFGVDAAGTLYDGVLLNDDWDDDSWDGVW
                     DGHVQIDSLGWTAELRIPYSQLRFHAAPQYVWGVNFSRDIARRHERDFLVYTPKNGSG
                     FVSRFPELVGITAVTPPPRLEVLPYTRARAEFSPHAAGDPFHDGSAMSPDAGVDAKLG
                     IGSNLTLNATVNPDFGQVEVDPAVVNLSDVETFFQEKRPFFIEGSSIFNFGRGGSNNY
                     WGFNWGDPDFFYTRRIGRAPQGSLPAADYADVPSGSHILGALKLTGKMGSSWNIGALS
                     ALTSREFAQLDTGNARFRAEVEPLASYTVARVQKEFPDGRQGLGLMTTLSARRFDDPR
                     LRDEISSGATAVGVDGWTFLDRSKTWVLTGWFGASDVRGTAARITALQENPQRYMQRP
                     DARNFHVDPAATSLAGWGARVYLNKQKGNWSSNSAIGALSPGFDVNDLGFLWRSGVVN
                     AHVQAGYRWSQPGKVFRSANTGGALFRSWDWDGDVTWTGAFQYGNLTLLNYYQLGWNV
                     AYNPQTMNDRRTRGGPLSINRPGYQVDVYANSDGRKSLVVSLSAGTYQSVQDRSAFVG
                     ASLQLRPASNVSLSIGPQYSWERTPVQYVTQYADSTATLTFGNRYVFAGLVANELSAS
                     LRLNWTFTPKLSLQFYGQPLISAGAYSDFKALERPKSFAFARWNDGTSTFDSTTFTPP
                     DNDFNFKSLRGNAVLRWEYLPGSTLYLVWTQSRTDVEDTGAFRFGPSLSRLTRSRPDN
                     IFMVKFTYWWTP"
     gene            complement(2668..3378)
                     /locus_tag="AC16JC_02100"
     CDS             complement(2668..3378)
                     /locus_tag="AC16JC_02100"
                     /inference="COORDINATES: protein motif:HMM:NF021371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjF family protein"
                     /protein_id="HIR5163520.1"
                     /translation="MTRPFLRAHWRDLVLLTYAVPDDLALPLVPSGCDADRWDGRCHV
                     SLVALRMERVRIRGLAVPGLTAYPLVNLRLYVRHAGRAAVYFVQELVPSGLLAAAARR
                     LYGEPFRAGPIRTRIEERNGAYLAEYTFGLDQPRWRVAVQADSGAEVPDGGSFAHWVK
                     ERVRGCRPDRFGQLHIFDVAHPPWAIRPVTAVELQVDFAALYGAAWAPLSDVRPDSIV
                     SAVGSDVTVSAPTVSTSR"
     gene            complement(3375..4184)
                     /gene="thiD"
                     /locus_tag="AC16JC_02105"
     CDS             complement(3375..4184)
                     /gene="thiD"
                     /locus_tag="AC16JC_02105"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HIR5163521.1"
                     /translation="MRIALTIAGSDSGGGAGIQADLKTFHQFGVFGTSVVCAVTAQNT
                     IGVRAWEPVPVSLVTAQLDALAEDLPPAAVKSGMLGTADVVEAVADAITRLRFPNYVL
                     DPVMVASSGDRLLAPDAAAVIARRLVPLATLVTPNLDEARILTEQEVRTPEQMERAGQ
                     ILLRQGAQVVLVKGGHLDGDTIVDVLVSARGIHRFTHPRNESVATHGTGCTLSAAIAA
                     GLALDRRLARAVEDAVDFVQRAIAAAPGLGQGRGPLNHFVPAPPRPPAEGL"
     gene            4233..>4776
                     /locus_tag="AC16JC_02110"
     CDS             4233..>4776
                     /locus_tag="AC16JC_02110"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF046830.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR5163522.1"
                     /translation="MGGDEARGPGGARITSNLTSTIRDLKRRKARGRRGLAIVEGIRL
                     VEEAVTARLRITGAVAGPDLARSSRGTALRIALENHAVPVEEVPARTFSELADTETPQ
                     GIIAVVEPRHWSPDDVSLDGSPSAPPPVLLVIDGVQDPGNVGTLVRTAHALGARGSVA
                     LRGTADVWGPKALRAAMGATF"
BASE COUNT          724 a         1638 c         1693 g          721 t
ORIGIN      
        1 ccgaccgacg tccaagggtt cgatccgcgc gcgccgtcac gcgcctcgag tcgcgctgtc
       61 caaccctgca gccggtcggg cgtatgacga cgatctcgtg tttcctgttg tgtctcgcag
      121 cggcagctcc cgtcccgtcg gtgacggtcg attcccccta catcgtggca ccgcaggtcc
      181 gcgcgatgcg ggccgttcag ccgctggtcg tggacgggcg gctggacgat ccggtgtggc
      241 gcacggcgga gcgcgtgagc gggttcctgc agcgggaccc gctggaaggg aaggccgcat
      301 cggaaagcac ggtcgtctac gtcgcctacg acgacgcggc gatctacatc ggcgcgcgga
      361 tgtacgacgc ccatcccgat tcgatcgtgg ggcggctggg ccgccgcgac gactacgcca
      421 actcggaccg cttcaacgtc tttctcgatc cctatcacga ccgccgcagc ggcgtgtact
      481 tcggcgtgga cgcggccggg acgctgtacg acggggtgct gctgaacgac gactgggacg
      541 acgattcctg ggacggggtg tgggacgggc acgtccagat cgactcgctc ggctggacgg
      601 ctgagctgcg gataccctat tcccagctcc gcttccacgc ggcgccgcag tacgtgtggg
      661 gcgtgaactt cagccgcgac atcgcgcggc gccacgagcg cgacttcctg gtgtacacgc
      721 ccaagaacgg cagcggcttc gtgtcacggt tcccggagct cgtgggcatc acggccgtca
      781 cgccgccgcc gcgtctcgag gtcctgccct acacgcgggc ccgcgccgaa ttctcgccgc
      841 atgcggcggg ggatccgttc cacgacgggt cggcgatgtc gcccgatgcg ggggtggacg
      901 cgaagctcgg catcggctcg aacctgacgc tcaacgccac cgtcaatccc gatttcgggc
      961 aggtcgaggt cgacccggcg gtggtgaacc tgagcgacgt ggagacgttt ttccaggaaa
     1021 agcggccgtt cttcatcgaa ggctcgtcga tcttcaattt cgggcgcggc gggtcgaaca
     1081 actactgggg cttcaattgg ggcgacccgg atttcttcta tacgcggcgc atcggccgcg
     1141 ccccccaggg cagcctcccg gcggcggact acgccgacgt gccgtcgggc tcgcacattc
     1201 tcggcgcgct caagctcacc gggaagatgg gctcgagctg gaacatcggc gccctgtcgg
     1261 ccctcacgtc gcgcgagttc gcgcagctcg ataccgggaa cgcccggttc cgcgccgagg
     1321 tggagccgct ggcctcctat acggtggcgc gggtccagaa ggaatttccc gatggccggc
     1381 aggggctcgg cctgatgacc acgctctccg cacggcgctt cgatgatcca cgcctgcgtg
     1441 acgagatctc cagcggcgcg accgccgtcg gggtagacgg atggacgttc ctcgaccgca
     1501 gcaagacgtg ggtgctgacc ggctggttcg gcgcgtccga cgtgcggggg acggccgcgc
     1561 ggatcaccgc cctgcaggag aatccgcagc gctacatgca gcgccccgac gcccgcaact
     1621 tccacgtcga tcccgcggcg acctcgctcg ccggctgggg ggcgcgcgtc tatctcaaca
     1681 agcagaaggg caactggtcc tcgaattccg cgatcggcgc cctctcgccc gggttcgacg
     1741 tcaacgatct cggattcctg tggcgctccg gggtcgtcaa cgcgcacgtg caagccggct
     1801 accgctggag ccagccgggc aaggtgttcc gctcggccaa taccggcggc gccctgttcc
     1861 gcagctggga ctgggacggc gacgtcacct ggaccggagc gttccagtac ggcaacctca
     1921 cgctgctcaa ctactaccaa ttgggctgga atgtcgccta caacccgcag acgatgaacg
     1981 accgccgcac ccggggcggg ccgctgtcga tcaaccggcc cggttaccag gtggacgtct
     2041 acgccaacag cgacggccgg aagtcgctcg tggtgagcct gtcggccggt acctaccagt
     2101 cggtacagga ccgttccgcc tttgtcggcg ccagtctgca gctgcggccc gcgtcgaacg
     2161 tgtccctgag catcgggccg cagtactcct gggagcggac ccccgttcag tacgtcaccc
     2221 agtacgccga ttcgacggcg accctgacgt tcggcaaccg gtacgtcttc gccgggctgg
     2281 tcgccaacga gctgtccgct tcgctgcggc tcaactggac cttcacgccg aaactctcgc
     2341 tccagttcta cgggcagccg ctgatctcgg cgggcgcata ctcggacttc aaggcgctgg
     2401 agcgtcccaa gagcttcgcg ttcgcccgct ggaacgacgg cacttcgacc ttcgacagca
     2461 cgacgttcac gccgccggac aacgacttca atttcaagtc gctgcggggc aatgcggtgc
     2521 tgcggtggga gtacctgccg ggctcgacgc tctatctcgt ctggacgcag agccgcacgg
     2581 acgtggagga cacgggggcg ttccgcttcg gaccctcgct ctcgcgtctc acccgctcgc
     2641 ggccggacaa catcttcatg gtgaaattca cctactggtg gacaccgtag gggcggagac
     2701 cgtcacgtcc gagccgaccg cagagacgat ggagtcgggc cggacatcgc tgaggggcgc
     2761 ccacgcggcg ccgtacaacg ccgcgaagtc cacctgcagc tccactgccg tgacgggccg
     2821 gatcgcccag ggcgggtgcg cgacgtcgaa aatgtgcagc tgtccgaatc gatcgggacg
     2881 gcagccgcgt acccgctcct tcacccagtg ggcgaacgag ccgccgtcgg ggacctccgc
     2941 tccggaatcg gcctggacgg ccacgcgcca gcgcggctga tcgaggccga aggtgtactc
     3001 ggccagatac gcgccgttgc gctcctctat ccgggtccgg atcgggccgg cgcgaaacgg
     3061 ctccccgtag agccggcgcg ccgcggcggc cagcagcccg ctcgggacca gctcctgcac
     3121 gaagtacacg gccgctcgac ccgcatgccg tacgtagaga cgcaggttga ccagcgggta
     3181 cgcggtgagt ccgggtacgg cgagcccacg gatgcgcacg cgttccatcc ggagggcgac
     3241 gagactcacg tggcagcggc cgtcccagcg gtcggcgtcg cagcccgagg gaacgagcgg
     3301 cagtgcgagg tcgtcgggga cggcataggt cagcagcacc aggtcgcgcc agtgcgcacg
     3361 cagaaaggga cgcgtcacag tccttccgcc ggcgggcggg gcggcgccgg aacgaagtgg
     3421 ttcagcggcc cgcgtccctg gcccagcccg ggcgccgccg cgatggcgcg ttgcacgaaa
     3481 tcgacggcgt cttccacagc gcgggcgagc cggcggtcca gcgcgaggcc ggcggcgatc
     3541 gcggccgaga gcgtgcagcc ggtgccgtgc gtcgcgaccg actcgttgcg cgggtgggtg
     3601 aagcggtgga tgccgcgcgc gctcaccagg acgtccacga tcgtgtcgcc gtccaggtgt
     3661 cctcctttca cgagcaccac ctgcgctccc tgacggagca ggatctggcc ggcgcgctcc
     3721 atctgctccg gtgtccggac ctcctgctcg gtgaggatcc gcgcttcatc gaggttcggg
     3781 gtcaccagag tggccagtgg gacgaggcgg cgggcgatca cggctgcggc gtccggtgca
     3841 agcaggcggt cacccgagga cgccaccatc accgggtcga gcacatagtt ggggaagcgc
     3901 aggcgggtga tggcatcggc gaccgcctcg acgacatcgg ccgtgccgag catgcccgac
     3961 ttcaccgccg cgggtgggag gtcctcggcg agggcgtcga gctgtgcggt caccagggac
     4021 accgggaccg gctcccaggc gcgcacgccg atggtgttct gcgcggtcac ggcgcagacg
     4081 acgctggtgc cgaagacccc gaactgatga aaggtcttca ggtcggcctg aatgccggct
     4141 ccgccgccgg agtcggagcc ggcgatggtc agggcgattc gcatgagcgc ttggcgccgg
     4201 aggcctgcgg ggcgaactta ccagggtggg cggtgggagg cgacgaggct cggggaccgg
     4261 gaggggcgcg catcaccagt aatctgacat ccacgatccg cgatctcaaa cggcgcaagg
     4321 cgcgcgggcg gcgcggcctc gcgatcgtgg aaggtatccg gctggtggag gaggccgtca
     4381 cggcgcgcct gcgcatcacc ggggcggtgg cgggtcccga tctcgcgcga tcctcgcgcg
     4441 gcaccgccct gcgcatcgcg ctggagaacc acgcggttcc ggtggaggag gtgccggcgc
     4501 ggacgttctc cgagctggcg gacaccgaga cgccgcaggg gatcatcgcg gtggtggagc
     4561 cgcggcactg gtcgcccgac gacgtgtcgc tcgacggctc cccgtccgcc ccgccgcccg
     4621 tgctgctggt catcgacggt gtgcaggatc cggggaacgt cggcacgctc gtgcgcaccg
     4681 cccacgcgct gggcgcgcgc ggctcggtcg ccctgcgggg cacggcggac gtgtggggcc
     4741 cgaaggcgct gcgcgccgcg atgggcgcca cgttcc
//
LOCUS       DBNGZW010000084         5549 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1234795, whole genome shotgun sequence.
ACCESSION   DBNGZW010000084 DBNGZW010000000
VERSION     DBNGZW010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5549
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1234795"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="AC16JC_02115"
     CDS             complement(<1..565)
                     /locus_tag="AC16JC_02115"
                     /inference="COORDINATES: protein motif:HMM:NF015237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HIR5163523.1"
                     /translation="MTAPPGKLHRFSRLLRKLIPVAILAVILLVVTSVGFIEYSARPG
                     FCKSCHIMQPYYDSWATSSHRDVACIKCHYAPGIRAEAMGKLQAANQVVKYITGTYGV
                     KPWAEIEDAACLRSGCHSERKVEGLVDYKGVRFDHASHLGELRRGKQLRCTSCHSQIV
                     QGQHVAVTDATCFLCHFKDRPPGAPIAG"
     gene            complement(562..1815)
                     /locus_tag="AC16JC_02120"
     CDS             complement(562..1815)
                     /locus_tag="AC16JC_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HIR5163524.1"
                     /translation="MSRLPVLAAIAVAAVALPSRALHATPAAVAPAASQLDTTYGCVA
                     CHADKRAAFVQGVHAERGIRCHDCHGGDPSAFTLPAAHRGGFVGAPGKVATVALCGTC
                     HSDPNRMRQYGLPSGQVAEFRTSRHGRLLLVGHDLDAPTCTDCHDAHTILRPDDARST
                     VYPTNIPTTCARCHEDKRLMAKYRLATDQVEQYRLSAHGVALFRNQNFASPTCVGCHG
                     SHSALPPTVTEIANVCERCHILVGQAFDRGPHGRAAHSGKIAGCLGCHSNHGTERVPA
                     DRIAAACAKCHAAGTSAYNLGTQIQVRAVQATEELRVAQVAIAQLSLAGRQTGDARFR
                     YQTAVTAYEQIAQAQHSLDVEHLDDLARQVRSISRDLRGMAGVAEEQRWEHKLLLLPV
                     WFLALAALVLAWFALRHLEGASGRP"
     gene            complement(1812..3095)
                     /locus_tag="AC16JC_02125"
     CDS             complement(1812..3095)
                     /locus_tag="AC16JC_02125"
                     /inference="COORDINATES: protein motif:HMM:NF012263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008121 - quinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HIR5163525.1"
                     /translation="MASRFGRATAQWAAQFWTDLKASTDAGLLGILRFLGLLYGPIDA
                     SLPIDQAFRKALGYRLAPHVGWRHALGGITYLLFIMLVVTGVLLSVHYRPSAQEAYPS
                     LQHIVSNVTLGWLIRDLHVWSASLLVLAVLAHMARVFFDGAYKPPRETNWLVGVLLLF
                     LVVAFGASGYLLPWDQWAYWTVTEALNALAAVPLIGGPAVAVLRGDPIVSGATLSRFF
                     AMHVIVLPWITLALVAFHFTMVRRHGVAPPKHAPGDPGSGRVFFPSHLLRSFMVAVLT
                     CAIVVSAAALWPRPIADPANPGKLPEAVRSTWVVVDVSRALTHYLGAWGFVGFTLVGL
                     GLAFVPLFDRGPERDLKRRPAVAALGLLFFVGFVAAWIAGRQLRSAPPPETLRAATGE
                     SMPAPAAGAPLQHLGPSPTRLTPPVDTARADRRSP"
     gene            complement(3096..3578)
                     /locus_tag="AC16JC_02130"
     CDS             complement(3096..3578)
                     /locus_tag="AC16JC_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR5163526.1"
                     /translation="MGSAPDAEKTTGGGRESRTPQAGFNEYLLVAVIALTILTVAVGI
                     GFYLLPGDYLQIPELQPAARVVRESDFPVGGSRVVSWGPRIVLVVRSGEREYSALQGT
                     SPIDGCMLDWDAASLRVVSPCTYVVYDLHGNVVRGLTTVPLQRFAVFVRQGAVYVTEL
                     "
     gene            complement(3683..5299)
                     /locus_tag="AC16JC_02135"
     CDS             complement(3683..5299)
                     /locus_tag="AC16JC_02135"
                     /inference="COORDINATES: protein motif:HMM:NF024827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5163527.1"
                     /translation="MTPSRWLSTALAVIALGACTNEKIVFKSRDNFNSPADTLHGFLG
                     YFDVSTKQTTCGNCHVEKQAEWKGTMHASAWDHQSAAVPNPAAACRACHTVTQLGNAV
                     PDSVKAGYALVADSSYYDVQCENCHGPGFDHAQAPNLSNIPLARVSLSPDTLVSVTSP
                     TCAACHNRSGGPAPSFVEEWSQSGHARVVPEIAAEGFPASCVACHEGRGVLKAWGVKT
                     NYVERDQAVTPATGFAQTCAVCHDPHANNNEGQLRWPIDDPDPANNLCMKCHNRRTDP
                     SASSPRGPHAPQGAVLLGYAGWWPDVRDTVAATHGSVTSNPRLCAGCHVNQITVSGGG
                     LPVAFKSVGHLFSPDPCLDANGTPTAGDCARPGDAGYVSGSRAFTGCALTCHNQSVTQ
                     VENAMSSQRAVIGQLADALWKDVHRDTVMYDTLPGGRFGAFDAGDTGLLTQLTAAQAD
                     TAFNFKDGKISVAEGVFFNVRLIGEARYANGDRSLGVHNPLLSQTLLSISIDALKQRY
                     GLAVPPAVQAVVDQTLRRVAQVRASRVGSR"
BASE COUNT          942 a         1870 c         1889 g          848 t
ORIGIN      
        1 acccggcgat cggcgcgccc ggcgggcgat ccttgaagtg acagagaaaa cacgtggcgt
       61 cggtcacggc gacgtgttgc ccctgcacga tctgggagtg gcagctcgta cagcgcagct
      121 gcttgcctcg acgcagctct cccaggtgtg acgcgtggtc gaaccgcacc cccttgtagt
      181 cgacgagacc ttcgaccttc cgttccgaat ggcaccccga ccgcaggcag gccgcgtcct
      241 cgatctccgc ccacggcttc accccgtagg tgcccgtgat gtacttgacc acctggttgg
      301 ccgcctggag cttccccatc gcctcggccc ggatccccgg cgcgtagtga cacttgatgc
      361 aggccacatc gcggtgggag gaggtcgccc aggagtcgta gtagggctgc atgatgtggc
      421 aggatttgca gaacccgggt cgcgcgctgt attcgatgaa gcccacgctc gtcacgacca
      481 gcaggatgac ggccaggatg gcgaccggga tgagcttgcg cagcagccgc gagaaccggt
      541 gcagcttgcc ggggggagcc gtcatggccg cccgcttgct ccttcaaggt gccgcagggc
      601 gaaccaggcc agcaccaggg cggccagcgc gaggaaccac acgggcaaca gcagcaattt
      661 gtgctcccaa cgctgctcct cggccacgcc cgccatgccg cgcagatccc gggagatcga
      721 acgaacctgt cgcgcgagat cgtccaggtg ttcgacgtcg aggctgtgct gcgcctgcgc
      781 gatctgctcg tacgccgtca ccgccgtctg ataccggaac cgggcgtcgc ccgtctgccg
      841 tccggcgagc gacaactgtg cgatggcgac ctgcgccacc cgcagctcct cggtcgcctg
      901 gacggcacgc acctggatct gggtgccgag gttgtaggcg ctcgtgccgg ccgcgtgaca
      961 cttggcgcac gccgccgcga tccgatcggc cgggacgcgc tccgtcccgt ggttgctgtg
     1021 acatcccagg catcccgcga tcttgccgga atgggcggcg cgcccgtgtg ggccccggtc
     1081 gaaggcctgc ccgaccagga tgtggcaccg ttcgcacaca ttggcgatct cggtgaccgt
     1141 cggcggcagc gcggagtggg agccgtggca ccccacgcac gtcggcgacg cgaagttctg
     1201 attgcggaac agcgcgaccc cgtgtgcgct caggcgatat tgctcgacct ggtccgtcgc
     1261 cagccggtac ttcgccatga ggcgcttgtc ctcgtggcac cgggcgcagg tcgtcgggat
     1321 gttggtggga tagacggtgc tgcgggcgtc gtccggccgc aggatggtgt gcgcatcgtg
     1381 gcagtcggtg caggtcgggg cgtcgagatc gtgaccgacc aacaggagcc ggccgtgccg
     1441 gctggtgcga aactcggcga cctgaccgct cggcagcccg tactgtcgca tgcggttcgg
     1501 atccgaatgg caggtgccgc acagggcgac ggtcgccact ttgccggggg ctccgacgaa
     1561 gccgccgcga tgcgccgcgg gcagggtgaa ggcggacgga tcccccccgt gacaatcgtg
     1621 acagcggatg ccgcgctccg cgtggacgcc ctgaacgaag gccgcccgct tgtcggcgtg
     1681 acacgcgacg cacccgtacg tggtgtccag ctgcgatgcc gccggcgcca cagccgccgg
     1741 cgtagcgtgc agcgctcgtg aggggagcgc gacggcggcc accgcgatcg cggcgagcac
     1801 cggaagccgg ctcatggcga cctccgatcc gcgcgggcgg tatcgacggg cggggtgagc
     1861 cgggtggggc tcgggccgag gtgctgcagg ggagctcccg ccgccggcgc cggcatcgac
     1921 tccccggtgg ccgcccgcag cgtctccggc ggcggcgccg agcgcagctg ccgacctgcg
     1981 atccaggccg ccacgaagcc cacgaagaag agcaatccga gcgcggcgac cgccggccgc
     2041 cgcttgagat cccgctccgg accgcggtcg aacaggggca cgaacgccag tccgagcccc
     2101 acgagggtga acccgacgaa cccccaggct cccaggtaat gcgtcagggc ccgcgagaca
     2161 tccacgacca cccaggtgga gcgcacggcc tccggaagct tgcccgggtt cgcgggatcg
     2221 gcgatcgggc gcggccagag ggccgcggcc gaaaccacga tcgcgcacgt cagcaccgca
     2281 accatgaacg accgcagtag atgactggga aagaacacgc ggccgctacc cggatcgccc
     2341 ggggcgtgct tgggcggggc gacgccatgg cgccggacca tcgtgaaatg gaacgccacg
     2401 agcgccaggg tgatccacgg cagcacgatc acgtgcatgg cgaagaagcg gctcagggtc
     2461 gcccccgaga cgatcgggtc gccccgcagc acggcgacgg cggggccgcc gatcaggggg
     2521 accgccgcca gcgcattcag cgcctcggtc accgtccagt aggcccattg gtcccagggc
     2581 agcagatacc cggaggcacc gaaggccacg acgagaaaca gcagcagcac gccgacgagc
     2641 cagttggtct cccgtggggg cttgtacgcg ccgtcgaaga acacccgcgc catgtgggcg
     2701 aggacggcca gcacgagcag gctcgccgac cacacgtgca ggtcgcgaat caaccagccg
     2761 agggtgacgt tcgagacgat gtgctggagg ctcgggtagg cctcctgcgc cgacggccgg
     2821 tagtggaccg acagcaggac cccggtcacg acgagcatga tgaagagcag gtaggtgatc
     2881 ccgccgagcg cgtgccgcca tccgacgtgc ggcgccagcc ggtagcccag cgccttgcgg
     2941 aacgcctgat cgatcggcag gctggcgtcg atcggcccgt agagcaggcc caggaaacgc
     3001 aggatgccga gcagcccggc atcggtgctc gccttcaggt cggtccagaa ctgcgccgcc
     3061 cattgcgccg tcgcccgtcc gaatcggctc gccatctata gctccgtcac gtacacggcg
     3121 ccctgtcgca cgaacacggc gaaccgctgc aggggcaccg tcgtgaggcc gcgcaccacg
     3181 ttcccgtgca ggtcgtacac cacgtaggtg cacggcgaga cgacgcggag cgacgcggcg
     3241 tcccagtcga gcatgcagcc gtcgatcggc gacgtcccct ggagggcgga gtactcccgc
     3301 tcgccggagc gcaccacgag cacgatcctc ggcccccagc tcacgacccg gctcccgccc
     3361 acggggaagt cggactcgcg caccacccgc gccgccggtt gcagctcggg gatctgcaga
     3421 tagtcgcctg gcagcaggta gaacccgatg cctaccgcaa ccgtgaggat ggtgagggcg
     3481 atgacggcga ccagcaggta ttcgttgaat cccgcctgtg gtgtgcggga ctccctccca
     3541 ccgcccgtcg tcttctccgc atccggagct gatcccatcg gccctccaac ccgctgcttc
     3601 gcagcccaaa acgcggcccc ggtcaccgtg aaggtgtccg gggccaggaa atcatacgac
     3661 gcgactcaac cggcgtcgag gatcagcggc tgccgactcg ggatgcacgg acctgtgcca
     3721 cacggcgcag cgtctggtca accaccgcct ggaccgctgg aggaaccgcc aggccgtagc
     3781 gctgcttcaa cgcgtcaatg ctgattgaca gcagcgtctg ggacagcagc gggttgtgca
     3841 cgccgagcga gcggtcgccg ttggcgtagc gggcttcccc gatcagccgg acgttgaaga
     3901 acaccccctc cgcaacagag atcttgccgt ccttgaagtt gaacgcggtg tccgcttggg
     3961 ccgcggtcag ctgtgtcagg agaccggtat cgcccgcgtc gaacgccccg aaccttcctc
     4021 cggggagcgt gtcgtacatc accgtatcgc ggtggacgtc cttccagagg gcatcggcga
     4081 gctggccgat caccgcacgc tgcgacgaca tcgcgttttc cacctgcgtg acactctggt
     4141 tgtggcacgt cagggcgcag ccagtgaaag ctcgcgaccc cgacacgtag ccggcgtcgc
     4201 caggacgggc gcagtcgcct gcggtcggcg ttccgttggc gtcgagacag gggtctgggc
     4261 tgaagaggtg accgactgat ttgaacgcca ccgggaggcc gccgcccgac accgtgatct
     4321 ggttcacgtg acagccggca cacagccgag gattggacgt cacgctaccg tgcgttgcgg
     4381 cgaccgtatc ccggacgtca ggccaccatc cggcgtaccc cagcagcacc gccccctggg
     4441 gagcgtgcgg gcctcgcggc gaactggcgg acgggtcggt gcgccggttg tggcacttca
     4501 tgcagaggtt gttggccggg tctgggtcgt cgatcggcca gcgcagttga ccttcgttgt
     4561 tgttagcgtg cggatcatgg cacaccgcgc aggtctgggc gaaccctgtc gctggggtga
     4621 ccgcctgatc tctttccacg taattggttt tgacgcccca ggccttcagg acgccgcggc
     4681 cctcatgaca cgccacgcag ctggcgggga atccttcggc tgcgatctcc ggcacgaccc
     4741 tggcgtgccc ggactgcgac cactcctcga cgaacgaagg ggccggcccg cctgagcgat
     4801 tgtggcacgc cgcgcacgtg ggcgaagtga ccgaaaccaa cgtgtcagga gagaggctga
     4861 cacgcgccag ggggatgttc gacagatttg gggcctgggc gtgatcaaag cctggtccat
     4921 gacagttctc gcactgcacg tcgtagtagc tcgaatccgc caccagcgcg tagcccgctt
     4981 tcacagagtc gggcacggcg ttccccagct gtgtcacagt gtggcaggct ctgcaggctg
     5041 ctgcagggtt gggtactgcc gcggactggt ggtcccaggc agacgcgtgc atggtgccct
     5101 tccactcggc ctgcttctcc acgtggcagt tgccgcatgt cgtttgcttg gtactcacgt
     5161 cgaaatagcc gaggaagccg tggagagtgt cggcagggga attgaagttg tctcggcttt
     5221 tgaaaacgat cttctcattt gtgcaggccc cgagcgcgat gaccgccagc gcggtcgata
     5281 accaacggga aggtgtcatc ggtgcatcct ccggttcatt acgccagacg ccagggctac
     5341 tgcatgagaa agcgggacgt cgacatcaca ttcatcaaat caagctcttg caagcactta
     5401 ggtccgtcgg accaggcttc gactgacccc tgctacttag gtagatatac tcacggaggc
     5461 tgcatgaaga aattgtgaag gaataatgac gctcccatca gcgccttcag tggcgctggc
     5521 cggtccttca ccaacccttc aggtggtcc
//
LOCUS       DBNGZW010000085         5237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_144251, whole genome shotgun sequence.
ACCESSION   DBNGZW010000085 DBNGZW010000000
VERSION     DBNGZW010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5237
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_144251"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="AC16JC_02140"
     CDS             complement(<1..546)
                     /locus_tag="AC16JC_02140"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5163528.1"
                     /translation="MPHPLVTIVVPCRNEERYIVECLDSILACDYPSNRLEVLVVDGM
                     SDDGTRAVLADYAARNPLVRVVDNPRRITPVALNLAIRAARGEVIVRMDAHVVYPRNY
                     VSRLVAALDEFGADNVGAVLRTLPANQTAMGKAIAIGMSHPFGVGTSYFRIGTDQPRW
                     VDTIAFFCIHRSTFDRVGMFDE"
     gene            complement(550..1608)
                     /locus_tag="AC16JC_02145"
     CDS             complement(550..1608)
                     /locus_tag="AC16JC_02145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5163529.1"
                     /translation="MEAPLRVLMITAGWPQPGQPQTTHFVKRQAEFLRKAGVVVDVFP
                     FRGGKRAWNYARAWREVRPRLTSGRYDLVHAQFGQSGVLALPKHLPLVVTLRGSDILG
                     IVGPDGRYTLAGRISQAVARFALRRADAVVVVSEHMTQYFRTPAPVTVLPSGIDFELF
                     RIVPRDEARARLGWPLDKRCILFAGNPDQPRKRYGLAKAAVDVLNRTLPAELVVAWGV
                     PHTDIPLYMNACDAFVFTSMQEGSPNVVKEALACDLPVVSVPIGDTAERLRGIEGCEL
                     VRDECPEAIAAALERVLRRGRRVAGRVAVAPLAEEAITARLIDVYRGALARHAGRRAR
                     PAADPANESVAATALPER"
     gene            complement(1610..2908)
                     /locus_tag="AC16JC_02150"
     CDS             complement(1610..2908)
                     /locus_tag="AC16JC_02150"
                     /EC_number="6.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate--amine ligase"
                     /protein_id="HIR5163530.1"
                     /translation="MTDRALVLDGQTNQALACVRSLGRAGYDVFVAATRRRPLASWSR
                     FSRDRFLLEDETPQAFAALRRWASARGVTLVLPLTERSCVLCNLERSAWEGDGIIVGS
                     APPELLALAFDKARTFELAQQAGVAIPPTRVPASLADARTAAEEVGYPCVVKSRFSNA
                     WDGSRFLPDPGTSYVRQRGELDAAVASHRQSGHWPVIQGFVPGQGKGVFALFESGRPV
                     AWFAHERLRDVRPSGSGSSLRRSAALDPRLLQPAERLLAAMGWHGPAMVEFRDDGVHA
                     PALMEVNGRFWGSLQLAVSAGADFPRWWAAILRRQPVERRTSYATGVTVRWVWGDVKR
                     FLYILAGAPSGYPGRYPTILQGLRELLGRQPRGTRSEAWQPDDRWPVVGEWVQGIGDL
                     LGGRRRRGSAASAAVLEPPTAATTAPSGGAQALQAVDERK"
     gene            complement(2905..3576)
                     /locus_tag="AC16JC_02155"
     CDS             complement(2905..3576)
                     /locus_tag="AC16JC_02155"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR5163531.1"
                     /translation="MSGGVLITFSGLDGAGKSTLIEFLTTTLEGRSQPVTVLHLNDQV
                     GVYAFLRGVRNRLRGAREDALAPGAPDPKSQKLQRPALRGLRGALSRLRTALLWNKGL
                     RRLLYPLDLAIFACYRAYLEQARGRVLIMDRYFYDTLVDLTNGRLGPWTWLLERITPR
                     PTVPVFLDITPEESFRRKREFTIEYLSRRADAYRRVFDRVPNAVRLPNDDLERTKAAL
                     LKAIP"
     gene            complement(3755..5068)
                     /locus_tag="AC16JC_02160"
     CDS             complement(3755..5068)
                     /locus_tag="AC16JC_02160"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR5163532.1"
                     /translation="MMRRRSHQNLYGPPAALAGAVTPGQPVVLQAVPQTAGPSRLLRW
                     MLYGFIFSLPFDAPGRLPLELTTLTGAAFLAVTLLQPGLCYGRRPFVFWLFFAYQYAY
                     WIAFVFGGAQFTSDAVHSSVFYIQGLLLFLACFNLMRDPRIARHVLLTLAIAVTILAV
                     MTIFGIGKAYDTESGRQIVLGQNANRAARVLCAGLLILVGMVFGRPQPGIRPKWIAWP
                     VAAIIGLAMILGGSRGGLVALAVGLWMFSFAGNTLGIRIRNTLVALFAIGIAAWGAWQ
                     SPVMQQRILLASQGNMAKREIIFPAAIQMFKSKPIYGWGPGNQYALAVRLGLPPALHQ
                     TRDTHNLFLEVLTATGLLGAIPYCTALWLCLWAAWKARRGFEGILPLAQMMALGVGNL
                     TGNYIQFKLQWVILAYALASWVYLTPQPQRVVMPTAADLRRRRWG"
BASE COUNT          879 a         1817 c         1784 g          757 t
ORIGIN      
        1 ctcgtcgaac atccccacgc ggtcgaacgt cgagcggtgg atgcagaaga acgcgatcgt
       61 gtccacccag cgcggctgat cggtcccgat ccggaaatag gacgtgccca ccccgaacgg
      121 gtgcgacatg ccgatggcga tggccttgcc catggccgtc tggttcgcgg gcagcgtgcg
      181 caacacggcg cccacgttgt cggcgccgaa ctcgtccagg gcggccacca gccggctgac
      241 gtagttccgc gggtagacga cgtgcgcgtc catccggacg atcacctcgc cccgtgccgc
      301 ccggatcgcc aggttgagcg ccacgggcgt gatgcgtcgg ggattgtcca ccacccgcac
      361 cagtggattc cgggcggcgt agtccgccag cacggctctg gtcccgtcgt cgctcatccc
      421 gtcgaccacc aggacttcga gccggttgga cgggtagtcg caggcgagga tcgagtcgag
      481 gcactcgacg atgtagcgct cctcgttgcg gcagggaacg acgatggtga ccagcgggtg
      541 aggcaccgct cagcgctccg ggagggcggt ggccgcgacg gattcgttcg cggggtctgc
      601 ggccgggcgg gcgcgccggc cggcatggcg ggcgagcgcg ccgcgataga cgtcgatcag
      661 ccgggccgtg atcgcctctt cggcgagcgg ggccacggcg acccggcccg cgacccggcg
      721 gccgcggcgc agcacgcgct ccagcgctgc cgcaatggcc tcggggcact cgtcgcgaac
      781 cagctcacag ccctcgatgc cgcggagccg ctccgccgtg tcgccgatcg gcaccgagac
      841 caccgggaga tcgcacgcca gggcctcctt caccacgttg ggtgagcctt cctgcatgga
      901 ggtgaacacg aacgcatcgc acgcattcat gtagagcgga atgtccgtgt gcggcacacc
      961 ccaggccacc acgagctctg cgggcagcgt gcggttgagc acgtccacgg ccgccttcgc
     1021 gagtccgtac cgcttgcgcg gctggtcggg gttgcccgcg aagagaatgc agcgcttgtc
     1081 gagcggccac cccaggcggg cacgtgcctc gtcgcgcggc acgatgcgga acagttcgaa
     1141 gtcgatcccg gacgggagca cggtcaccgg ggcgggcgtc cggaaatatt gggtcatgtg
     1201 ctcggagacg acgaccacgg cgtcggcgcg ccgcagggcg aagcgcgcga cggcctgcga
     1261 gatccggccc gccagcgtgt agcggccatc cggtcccacg atgcccagga tgtcgctgcc
     1321 gcgcagggtg accaccagcg gcaggtgctt gggcagcgcc agcacgccac tctggccgaa
     1381 ctgtgcgtgc accagatcgt agcggccgga cgtgagccgc gggcgcacct ctcgccaggc
     1441 gcgcgcgtag ttccaggcgc gcttgccgcc gcgaaatggg aacacgtcca cgaccacccc
     1501 cgccttgcgc aggaactccg cctgtcgctt gacgaagtgg gtcgtctgtg gctgtcccgg
     1561 ttgcggccag ccggccgtga tcatcagcac gcgtaacggt gcttccattt catttccttt
     1621 catcgacggc ctggagcgcc tgcgctccgc cggacggtgc cgtggtggcc gcggtgggag
     1681 gctcgagcac cgcggcggaa gctgcgctac ctcggcggcg ccggccgccg agcaggtcgc
     1741 cgattccctg gacccattct ccgacaacgg gccagcgatc gtcgggttgc caggcctccg
     1801 agcgcgtgcc ccgcggctgc cgtcccagga gctcccgcag tccctgcagg atcgtgggat
     1861 atcgccccgg gtaccccgac ggcgcgcccg cgaggatgta gagaaaccgc ttcacgtccc
     1921 cccataccca gcgcaccgtc acaccggtgg catagctggt gcggcgctcc accggctgcc
     1981 ggcgcagaat cgcggcccac caccggggga agtccgcccc tgcggagacg gccagctgca
     2041 aggagcccca gaaccggccg ttgacttcca tcagggcggg cgcatgcacg ccgtcgtcac
     2101 ggaactcgac catcgccggg ccgtgccagc ccatcgccgc gagcaaccgc tcggcaggct
     2161 gcagcagccg tgggtcgagg gccgccgagc ggcggaggct gctgccggac cccgaagggc
     2221 ggacgtcgcg caaccgctca tgggcgaacc aggcgaccgg tcggcccgac tcgaacagag
     2281 cgaacacgcc cttgccctgt ccgggcacga agccctggat caccggccag tggccactct
     2341 gacggtggga ggccacggcg gcgtcgagct cgccccgctg gcgcacgtag ctcgtcccgg
     2401 gatcggggag gaatcgcgat ccgtcccagg cattgctgaa ccgcgatttc acgacgcagg
     2461 ggtagccgac ttcctcggcc gccgtgcgag catcggccag tgaggccggc actcgcgtcg
     2521 ggggaatcgc cacccccgcc tgctgggcca gctcgaaggt ccgggctttg tcgaacgcga
     2581 gcgccagcag ctccggcggc gcgctgccca cgatgatccc gtcaccctcc caggcggatc
     2641 gctccaggtt gcacaacacg caggagcgct cggtgagcgg caacaccagc gtcacgccgc
     2701 gggcgctcgc ccatcggcgc agcgccgcga acgcctgcgg cgtttcatcc tccagcagga
     2761 atcggtctcg ggaaaaacgc gaccaggacg ccagcggccg gcgccgcgtg gcggccacga
     2821 acacgtcgta gcctgcccgg cccagcgagc gcacgcatgc cagggcctgg ttcgtttgcc
     2881 cgtcgaggac cagcgcgcgg tcggtcacgg gatcgctttc agcagcgcgg ccttggtgcg
     2941 ctcgaggtcg tcgttcggca accggacggc gttcggcacc cggtcgaaca cgcgccggta
     3001 cgcgtcggcc cggcgactca ggtattcgat cgtgaactcg cgcttccgcc ggaatgattc
     3061 ctcgggggtg atatcgagaa acaccggcac ggtggggcgc ggcgtgatcc gctcgagcaa
     3121 ccaggtccag ggccccagtc ggccgttggt gaggtctacc aatgtgtcgt agaagtagcg
     3181 gtccatgatc agcacgcgcc ccctggcctg ctcgaggtag gcgcgatagc aggcgaagat
     3241 cgccaggtcc agcgggtaga gcagccgccg caaccccttg ttccagagca gcgcggtccg
     3301 cagccggctc agcgcgccgc gcagaccgcg cagggccggt cgctgcagct tctgcgactt
     3361 ggggtcgggg gctcccgggg cgagcgcgtc ctcgcgcgct ccccgcaggc gatttcgcac
     3421 cccccgcagg aacgcgtaca cgccgacctg atcgttgaga tgcagaacgg tgaccggctg
     3481 gctccggccc tccagcgtgg tggtcaagaa ctcgatgagc gtggacttgc ccgctccatc
     3541 cagaccagaa aacgtgatca gcacgccccc cgacatcctc cggttcgtcc ctccgcaaac
     3601 tcacagatat tgcctacgca ccccgcgtgc tagcaggacg acgcggcgtc ttgatgtaac
     3661 ctatttgcca acaagcggtt ataccgtgaa ccccgtgtgg gaatctgctg gcgaggactg
     3721 ttgtggcgtg gccgcaacag acggtaagtg tccgctaccc ccagcggcga cgtcgcaagt
     3781 ctgcggccgt cggcatcacc acccgttgcg gctgcggcgt gagatacacc cagctggcca
     3841 gggcgtacgc cagtatcacc cattgcagct tgaactgaat gtagttgccg gtcaggtttc
     3901 ccaccccgag cgccatcatt tgggccagcg gcaggattcc ttcgaacccg cgcctggctt
     3961 tccaggcggc ccagagacag agccacagcg cggtgcaata cgggatcgcc cccaggaggc
     4021 ccgtcgccgt cagcacctcg aggaacaggt tgtgagtatc gcgcgtctgg tgcaaggcgg
     4081 gcgggagacc cagtcgcacc gcgagcgcgt actgatttcc cggaccccat ccgtagatcg
     4141 gtttgctctt gaacatctgg atggcggcag ggaagatgat ctcgcgcttc gccatgtttc
     4201 cctggcttgc cagcaggatg cgttgttgca tcaccggcga ctgccatgcg ccccaggccg
     4261 cgatgccgat cgcgaacagg gccaccagcg tgttccgaat ccggatgccc aacgtgttcc
     4321 cggcgaacga gaacatccac aggccgactg ccagcgccac gagcccgccc cggcttccgc
     4381 ccaggatcat cgccagcccg atgatggcgg ccacgggcca ggcgatccac ttggggcgta
     4441 tcccgggctg ggggcgcccg aacaccatgc cgaccaagat caggaggcca gcgcacagca
     4501 cacgggcggc gcgattggcg ttctgcccga gcacgatctg ccgtcccgat tcggtgtcgt
     4561 acgccttccc gatgccgaag atggtcatga cggccaggat ggtcaccgcg atcgccagtg
     4621 tcagcagcac atggcgggcg atgcgcggat cgcgcatgag attgaaacag gcgaggaaga
     4681 ggaggagacc ttggatgtag aagacggatg agtgcaccgc atcagaggtg aactgcgcgc
     4741 caccgaacac gaacgctatc cagtaagcgt actggtaggc gaagaacagc cagaagacga
     4801 acggacgccg cccatagcac aaccccggct gcagcagggt gacggcgagg aacgccgcgc
     4861 cggtgagggt cgtgagctcg agcgggaggc ggccgggagc gtcgaacggc agcgagaaga
     4921 tgaacccgta gagcatccag cgaaggagtc ggctggggcc tgccgtctgt ggcacggcct
     4981 gcagcaccac gggctggcca ggggtgaccg ctccggccag tgcggccggc gggccgtaca
     5041 ggttctggtg actcctccgt cgcatcatgg gatctccgct cagtccctgc gccgcaaccc
     5101 gagatgctcc aattcggtca acagccgacc ggcagccacg tcgaccgcga gaccggtggt
     5161 gtccaggtgc accatgccaa cgcgcttgcc cgtcgtcagg ctgcccaaga tctcgcgata
     5221 cgcccggcga tagcgct
//
LOCUS       DBNGZW010000086         1955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_826021, whole genome shotgun sequence.
ACCESSION   DBNGZW010000086 DBNGZW010000000
VERSION     DBNGZW010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1955
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_826021"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            204..>1955
                     /locus_tag="AC16JC_02165"
     CDS             204..>1955
                     /locus_tag="AC16JC_02165"
                     /inference="COORDINATES: protein motif:HMM:NF020698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasin domain 3-containing protein"
                     /protein_id="HIR5163533.1"
                     /translation="MPILATLAILPLASCNDDRSLTQPPGRTRYTTPTGLVTVNPPQI
                     LVGAGDISECGTNNDDEATAQLLDVISGTVFTAGDNVMPNGTTSEFANCYDPTWGRHK
                     ARTKPAAGNHDYNTSGAAGYFAYFGAAAGEPGKGYYSYALGAWHIVVLNSNIATAAGS
                     AQDTWLKADLAAHPNQCTLAYYHHPLYSSASGDGSGGATLSAVRPFVNALYAAHADLV
                     MNGHRHIYERIAPMKPDGSADPALGLREFIVGTGGDGGGSPTNVFPLSEVRNGDTRGV
                     FKLYLYDDSYAWKFVPIAGKTFTDSGSAACHVSGGGGGGGVSPTTSTVLAVPASFTAG
                     SGFSTITVTAKDGNGNPVGGAGVTLATSGSGNTLTPTSGTTDGTGVFTSVLTSAVAEG
                     KIVSATIDGTPINQTASVTVTPSGGGGGGGGTITQTLLTSGSNRANQRVYTTASVSPT
                     PNTLITVAVRSRRSGGALTPTVSGGGMTAWTQVASVDYDVVTAPLARLVVFRAMSAAP
                     GSGPITITYSNSVSNADWVVSQWTGVDLSGANGSGAIGQTGSARGNAVSALSSALAPF
                     GDAGNVAFGVLAAATNGP"
BASE COUNT          337 a          727 c          597 g          294 t
ORIGIN      
        1 gtaggaatcg aacctaccga ggcccttgcg aaccccagct ggttttgaag accagtcagg
       61 ccaccaggcc cgatccgccc ccgtcggttt tcaccttctg ttcaccaaga gttccgcctg
      121 tcttcaactc gtcttgcgac acgggctggt agtgtatatg acgaccctcg gagtctctca
      181 tgggcccggt gtcacggaag ttagtgccaa ttctcgctac cctggctatc ctgccgctcg
      241 cctcctgcaa cgatgaccgt tcgctcacgc aaccccccgg aaggacccgg tacaccaccc
      301 ccaccggcct cgtgacggtg aacccgccgc agatcctcgt cggggcgggt gacatctcgg
      361 agtgcggcac caacaacgac gacgaagcga cggcgcagct gctcgacgtc atctccggga
      421 cggtgttcac ggcgggcgac aacgtcatgc ccaacggcac cacgtcggag ttcgccaact
      481 gttacgatcc gacctggggc cgccacaagg cgcgcaccaa gccggcggcc gggaatcacg
      541 actacaacac ctcgggcgcc gccggatact tcgcctactt cggcgccgcc gcgggcgagc
      601 ccggcaaagg gtactacagc tacgccctgg gcgcctggca catcgtcgtg ctgaacagca
      661 acatcgccac cgccgccgga tcggcccagg atacctggct caaagccgat ctcgctgctc
      721 acccgaacca gtgcacgctc gcctactatc accacccgct ctactcctcg gccagcggcg
      781 acggatccgg cggggcgacg ctgtccgccg tccgaccctt cgtcaacgcg ctgtacgccg
      841 ctcacgccga tctcgtcatg aacgggcacc ggcacatcta cgagcggatc gcgccgatga
      901 agcccgacgg cagcgcggac cccgcactcg gcctccgtga attcatcgtc gggacgggtg
      961 gcgacggcgg cggcagcccg accaacgttt tccccctgag cgaggtgcgc aacggcgata
     1021 cccgtggcgt gttcaagctg tacctgtacg acgacagcta cgcctggaaa ttcgttccca
     1081 tcgcgggcaa gacattcacc gattccggat ccgccgcgtg ccacgtctcc ggcggcggtg
     1141 gtggcggcgg cgtctccccc acgacctcga ccgtcctggc cgtgccggcc tcgttcactg
     1201 cgggcagcgg cttctccacg atcacggtga ccgcaaagga cggcaacggg aaccccgtcg
     1261 gcggtgccgg cgtgacgctg gcgacatcgg ggagcggcaa taccctcacc ccgacctccg
     1321 gaacgaccga cggcacgggg gtcttcacga gcgtcctgac ttccgccgtc gcggaaggaa
     1381 agatcgtctc ggcgacgatc gacggcacgc cgatcaatca aactgcttcg gtgaccgtaa
     1441 caccgtccgg cggcggcggg ggaggcggcg gcaccatcac gcagacgctc ctgacatcgg
     1501 gcagcaaccg cgcgaatcag cgcgtgtaca cgaccgcgtc cgtctccccg accccgaaca
     1561 cgctcatcac ggtcgccgtg cgcagtcgcc gcagcggcgg cgccctcacc ccgaccgtca
     1621 gcggcggtgg gatgaccgcc tggacccagg tcgcgagcgt ggactacgac gtggtcacag
     1681 caccgctggc gcgtctggtc gtgttccggg ctatgagcgc tgcacccgga agcggaccga
     1741 tcaccatcac ctactccaac agcgtctcca atgccgactg ggtcgtgtcg cagtggaccg
     1801 gcgtcgatct gagcggcgcc aacggctcgg gcgccatcgg gcagacgggc tccgcccgcg
     1861 gcaacgccgt ctccgcgctg agcagcgccc tcgccccgtt cggcgacgcc ggcaacgtcg
     1921 ccttcggcgt actcgccgca gccaccaatg ggccg
//
LOCUS       DBNGZW010000087         6392 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_485875, whole genome shotgun sequence.
ACCESSION   DBNGZW010000087 DBNGZW010000000
VERSION     DBNGZW010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_485875"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1539)
                     /locus_tag="AC16JC_02170"
     CDS             complement(<1..1539)
                     /locus_tag="AC16JC_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH36-type glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HIR5163534.1"
                     /translation="MFGRFGGRRRRKVEAPLRAELLSIERLEERAKALAASFTLARNP
                     RRKARPFFSRLDDNAGVLREAYRTCSDDVHRGAFVPPAAEWLLDNFHIIDGVIRDTRH
                     DLPQQYYLQLPKLASREMAGIPRIYAMALDLIRHTDGRLERQQLVRFVTAYQTVAPLT
                     IGELWAWPSVLKLALIESLRRLADETLQGRDARLNADRYLERIGDADERAPLASLPEV
                     LETAYVVRLLQRMREYGPLVSPVRAAVEERLAAQGMTAEDAIRTEYQTQAAAQVSFGN
                     GITSLRLCATLDWTQYFESVSLIEQVLQRDPAGVYGRMDFQSRDRYRQAVEELAEPTG
                     EAQLRVALRSVESARQAAEQQSSQDPTAHVGYHLIGSGRPDLEADVEYRPGLADRARR
                     FLFAHVTIFYLGSIGLVTGALLALAVAYVRAQGGSLWTQAGIAALLLLPASEFAIAFV
                     QRLVAHLVTPRRLPRLDFQAGVPEDARTMVVVPTLLTSVAGVAELLEHVEVLALGNVD
                     PRIHF"
     gene            1784..2536
                     /locus_tag="AC16JC_02175"
     CDS             1784..2536
                     /locus_tag="AC16JC_02175"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5163535.1"
                     /translation="MSVQGPTETIARPQAPAGETSRREPFENDHVLVIDSIYAPGGSV
                     PLHTHRSPHVVYVIEGGILETTGPEGSVETHEVRPGQTLWRAPQSHRTRNVGGTRVRI
                     VEVEVKQASRTPALWEKAPPIVTSAEHGWVPDPFDPRRSIAMLVGDPTKPGPYTLRYR
                     APAGYKIGLHLHPDEDEQLTVLSGSIHWSPGAAGTGAPEYTLTAGGFAPVRAGTPHRL
                     WTTEECVLQMHGIGPRTYVYLNPADDPRAATV"
     gene            complement(2618..3448)
                     /locus_tag="AC16JC_02180"
     CDS             complement(2618..3448)
                     /locus_tag="AC16JC_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163536.1"
                     /translation="MKTRELATKVLVTWVYLVAVQTMLGMLITSPTPVPENAGPWFLL
                     SNFLIALVLCLLAMRSDWGGARLAVAVSSIPAIIMLTNYLEGIVFLTGVTIDWPKEVL
                     RTCMVSVLTMPLWPLLFRKRGVAQHNFRPLAGRTVKERIWRLVLADLAYPVLYFLAGT
                     LVFPFVRDFYATQTIPAVGLVFTLQLLVRGPIFLGVCLLMTRMWGLARLPGAAAVGAA
                     FATLSGIAPLLIPSGVFPEPVRWAHFIEVTTSNFIFGLLVAWLWGPANRVRQMVSQAA
                     "
     gene            complement(3456..3968)
                     /locus_tag="AC16JC_02185"
     CDS             complement(3456..3968)
                     /locus_tag="AC16JC_02185"
                     /inference="COORDINATES: protein motif:HMM:NF015513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR5163537.1"
                     /translation="MGLLHDNPTSGYALRKVVALTPMSAFSDSPGAIYPALRRLRKAG
                     LIRGRLERSSGLRRRQVFQLTPAGIRELKQWLALPPSRDEVMRRLEELSLRFAFMDQV
                     MGEHASVQFLRAMRRELEAYVPSLHDYLAAAKSQMPLSARLALQSGVRGYETQLRWTR
                     EALAAYDKEK"
     gene            complement(4332..4730)
                     /locus_tag="AC16JC_02190"
     CDS             complement(4332..4730)
                     /locus_tag="AC16JC_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5163538.1"
                     /translation="MRTRILTTLAGMVLGAATTGWGQQPADTMAITPGLIALGDSIFH
                     GKVAGGLCFGCHGVDGKGTPGLAPDLTSGKWLHGDGSLASIIKTVEAGVPHPKTAPAP
                     MPPMGGSKLGPAEVRAVAAYVFSLSHPKAH"
     gene            complement(4763..6283)
                     /locus_tag="AC16JC_02195"
     CDS             complement(4763..6283)
                     /locus_tag="AC16JC_02195"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5163539.1"
                     /translation="MRTRHAAHLCGLLGLLGLLGLLGGRVGAQVVGVFPGTPTVGARI
                     FVDRGCVHCHSIWGNGGTLGPDFAVVGAGRSMQQLAGEFWNHTPRMIVTVRRQGVRWP
                     TFTESELADVISYIYYVKLFDPPGDADLGAQWFREKRCVECHAVGGTGGHVGPALDGY
                     ARYIAPIMLAEGMWNHGPAMQEAQTEHHIPIPTFLGREMADIQAYIRSASSLRDRAVV
                     LLQPPNPANGERLFSAKGCVRCHGPGGRGTRYAPDLRTATLRLSVSEIAGVLWDHSFR
                     MSAAMQARGFAFPRFQGTEMADVIAFLYYLRFNETGGDAREGAQVFAHKGCTTCHFLD
                     GRPATGPDLTRAAAVRTPLGLATAMWNHAPAMFDVAQSRQVEWPGFEGDEMRDLSAFL
                     HTLQPPAPARAASAANPPARRSAAALPPIDSALAAEGQALAAQRACAACHVLGASTMG
                     RVGPDLNTVFQRRSPEYVLHKLADPKFDNPKSLMPQFPLDEGPRRAILEYLRMLER"
BASE COUNT         1092 a         2146 c         2168 g          986 t
ORIGIN      
        1 gaagtgaatg cgggggtcga cgtttccgag cgccaggacc tccacgtgct ccagcagctc
       61 ggccaccccg gccacgctgg tgagcagggt gggtaccacg accatggtcc gcgcgtcttc
      121 gggcacgccg gcctggaagt cgaggcgggg caagcgccgc ggcgtgacga gatgcgcgac
      181 caagcgctgg acgaaggcga tggcgaactc gctggcgggt aacagcagca gcgccgcgat
      241 cccggcctgg gtccataggg agccaccctg cgcccggacg tatgccacgg ccagcgccag
      301 cagggcgccg gtgacgagtc cgatcgatcc caggtagaag atggtgacgt gagcgaacag
      361 gaagcgccgg gcgcggtcgg cgagtccagg gcggtactcc acgtcggctt cgaggtcagg
      421 tcgcccgctc ccgatcaggt gatagcccac gtgggcggtg gggtcctggg acgattgctg
      481 ctccgcggcc tggcgggcac tctccacgct gcgcagggcc acgcggagct gggcctcgcc
      541 ggtgggctcg gccagctcct ccaccgcctg ccgatagcgg tcccggctct ggaagtccat
      601 tctcccgtac acgccggccg gatcgcgctg cagcacctgt tcgatgaggc tgacgctttc
      661 gaagtactgg gtccagtcca gggtggcgca caaccgcagg ctggtgatgc cgttgccgaa
      721 ggagacctgg gcggccgcct gcgtctgata ctcggtgcgg atcgcatcct cggcggtcat
      781 gccttgggcg gcgaggcgtt cctcgacggc cgcgcggacg ggcgagacga ggggaccata
      841 ctcgcgcatc cgctgcagca gccgcacgac gtaggccgtc tccaggactt cggggaggga
      901 cgccaagggc gcccgctcgt cggcatcgcc gatccgctcg aggtagcggt ctgcgttgag
      961 acgcgcgtca cgaccctgga gcgtttcgtc cgccagccgc cgcagactct cgatgagggc
     1021 gagtttgagc acgctgggcc aggcccagag ctcgccgatg gtcaggggcg ccaccgtctg
     1081 gtaagccgtg acgaagcgca cgagctgctg tcgctccagg cgaccgtcgg tatggcggat
     1141 caggtcgagc gccatggcgt agatccgggg gatcccggcc atctcccggg aggcgagctt
     1201 gggcaactgc aggtagtact gctgcggcag gtcgtgccgg gtatcccgga tcacgccatc
     1261 gatgatgtgg aagttgtcga gcagccactc ggccgccggg gggacgaacg cgccacggtg
     1321 gacgtcgtcg gagcaggtac gatacgcctc gcgcagcacg ccggcgttgt catccaggcg
     1381 cgagaagaac gggcgggcct tgcggcgggg gttccgggcg agcgtgaagg atgcggcgag
     1441 ggccttggcc cgttcctcca atcgctcgat gctcaggagt tcggcgcgga ggggcgcttc
     1501 gaccttccgg cgacggcgac ccccgaagcg gccgaacaac ggcgttctcg ttgtgagacg
     1561 tggtccttcg gcgatagtcc ttggctccgt tccgggtgtc gaatcgaatg ataaccggtt
     1621 cacgggtggt gaatcatgac ttcctctggg aatggatggc gtgtagtctg aggcgcggcg
     1681 cgtcgggtga agagcatgtg aaagccaagc cttctcaccg ccggctcatc cctccttcac
     1741 ctgccctgcg ctaccgtgcg ctcatgacat ggtggaggac acgatgagcg ttcagggccc
     1801 gacggagacg attgcccgcc cccaggcgcc ggcaggtgag acgagccgga gagagccctt
     1861 cgagaatgac cacgtgctgg tcatcgacag catctacgcc cccggtggaa gtgtgccgct
     1921 gcacacgcac cgctcgcccc acgtggtgta cgtcatcgaa gggggtatcc tcgaaacgac
     1981 cggccccgag ggcagcgtcg aaactcatga ggtgcggccg gggcagacgc tctggcgggc
     2041 accgcagtcg catcgcacgc gcaacgtcgg aggcacgcgc gtacggatcg tggaagtcga
     2101 ggtcaagcag gcctctcgaa ccccggccct gtgggagaag gcgccgccca tcgtgacatc
     2161 cgccgaacat ggatgggtgc cggatccctt cgacccgcgg cgcagcatcg ccatgctcgt
     2221 cggcgatccg accaagcccg ggccgtacac gctgcgctat cgtgcgccgg ccggctacaa
     2281 gatcggactc cacctccacc ccgatgagga cgagcagctg acggtcctct cgggatccat
     2341 ccactggtcg ccgggcgcgg cgggcaccgg cgctccggag tacacgctga cggcaggagg
     2401 cttcgcgcca gtgcgcgcgg ggacgccgca tcggctctgg actaccgagg agtgtgtgct
     2461 ccagatgcac ggcatcgggc cacgcacata cgtctatctc aatccggcgg acgatccgcg
     2521 ggccgcaacg gtttgagccg ccggccacct gtggctccgg tgactgaacc tcgcgcgacc
     2581 cctcgcggca ccgttcagac caatcctctc cttgttttca ggcggcctga ctcaccatct
     2641 gcctcacccg atttgctggt ccccagagcc aggccacaag cagaccgaag atgaagttgg
     2701 acgtagtgac ctcaatgaag tgagcccagc gcaccggttc gggaaagacg ccggagggta
     2761 tgagcaaagg agcgatgccg ctgagagtgg caaaggctgc tccgaccgct gcagcgcccg
     2821 gcaatcgagc caggccccac atgcgcgtca tcagcaggca aacgccgaga aagatgggcc
     2881 cccgcaccag tagttgcagc gtaaacacca ggcccactgc cgggatcgtc tgagtggcgt
     2941 aaaaatcgcg gacgaaagga aagaccagcg ttcccgccag gaagtaaagg accggatagg
     3001 caagatcggc gagcacgagc cgccagatcc tctccttcac cgttctgccc gccagggggc
     3061 ggaagttgtg ttgcgccact ccccgcttac ggaaaagcaa cggccacaac ggcatggtga
     3121 ggacgcttac catgcacgtc cgcaggactt ccttgggcca gtcaatcgtc acgccggtaa
     3181 gaaagacgat cccttccagg tagttggtca gcattatgat ggctggaatg ctggacaccg
     3241 ccaccgccag gcgcgcgcct ccccaatccg accgcatcgc cagaaggcaa agcaccagcg
     3301 caatcaagaa gttgctgaga aggaaccaag gcccggcgtt ctcgggcacg ggggtaggac
     3361 tggtgatcag catccccagc atcgtttgaa cggcgaccag gtacacccag gtcacgagga
     3421 ctttcgtggc cagttctctc gtcttcatac gcgcctcact tctctttgtc gtacgcagct
     3481 agagcctcgc gagtccaccg gagctgggtc tcgtatcctc ggactccgct ttgcagcgcc
     3541 agtcgtgccg agaggggcat ctggctcttg gcggccgcca ggtagtcgtg gaggctggga
     3601 acataggcct ccaattctcg cctcatcgcc ctgaggaact gaaccgaggc gtgctcgccc
     3661 atcacctgat ccatgaatgc aaaccggaga gacagttcct caaggcggcg catgacctcg
     3721 tcacggctcg gagggagagc gagccactgc ttcagttcac gaatgcccgc cggggtcagc
     3781 tgaaacacct gtcgccggcg caagccggag cttctctcca ggcggccgcg gatgaggccg
     3841 gccttccgca ggcggcgcag tgcgggatag atcgctcctg ggctgtcgct gaaggcgctc
     3901 atgggagtga gcgcgacgac tttcctgagg gcgtaccccg aggtggggtt gtcgtgcagc
     3961 agtcccaaca gggcatatcc caggaggccg aattctggtt ttctcatagt acggaccata
     4021 tggtactacg agtactatcg aaagaacaag gccaggggcc gcctggctag ccgggacccg
     4081 ttcggtgcgg tggttgcgtg gcagttcgac cgggcaccgg ccagacaccc gggggatcgc
     4141 cccgcgcagc ggtgcccgta actccccgtg cgaagagcgc aaacgctctg gccaaacgct
     4201 ttggagggcg agccgccaac ggggttagct gcgcgcaccg gtccacccga acggttctct
     4261 cgacgccagc cgcgagggcg tagctcgggc cgcccacgct ccatccagtc agcgaggctc
     4321 ccccccccgt gctagtgcgc tttcgggtgg ctcaatgaga agacgtacgc ggccacggcc
     4381 cgcacctccg caggcccgag cttcgatccc cccatgggcg gcatcggcgc tggagcggtc
     4441 tttgggtggg gcacgccggc ttcgacggtc ttgatgatgg aggctaagct accatcgccg
     4501 tggagccatt tgccgctcgt caagtcgggc gcgaggccgg gggtgccctt cccgtccacg
     4561 ccgtggcagc cgaagcagag gccgccggcc accttcccat ggaagatgct gtcgcccagc
     4621 gcgatcaacc ccggcgtaat cgccatggtg tcggcgggct gctgccccca cccggtcgtg
     4681 gctgccccca ggaccatccc tgccagcgtg gtgagaattc gtgtgcgcat cgcggcccct
     4741 ttcctcatat aggacgacta agtcatcgct cgagcatgcg aaggtactcg aggatagcgc
     4801 ggcgcgggcc ctcgtccagc gggaactgag gcatcaggct cttggggttg tcgaacttcg
     4861 ggtccgcgag cttgtgcagc acgtactcgg gcgagcgccg ctggaacacc gtgttcaggt
     4921 cgggaccgac gcggcccatg gtggacgccc ccagcacgtg gcacgcggcg caggcgcgct
     4981 gggcagcgag ggcttgcccc tctgccgcca gcgccgaatc gatgggcggc agcgccgccg
     5041 ccgaccggcg tgccgggggg ttggcggcgg aggcggcacg ggccggcgcg ggcggttgca
     5101 gcgtgtggag gaacgccgac agatcgcgca tctcgtctcc ctcaaacccg ggccactcca
     5161 cctggcgcga ttgcgccacg tcgaacatcg ccggggcgtg gttccacatc gcggtcgcga
     5221 ggccgagcgg cgtgcgcacc gctgcggcgc gcgtcaggtc cggaccggtc gccggccggc
     5281 cgtcgaggaa gtggcaagtg gtgcacccct tgtgagcgaa cacctgggcg ccctcgcgcg
     5341 catccccacc ggtctcgttg aaccgcaggt agtagaggaa cgcgatcacg tcggccatct
     5401 cggtcccctg gaaccgcgga aaggcgaacc cgcgcgcctg catcgcggcc gacatccgga
     5461 acgagtgatc ccacagcacg ccggcgatct ccgacacgct gagccgcagc gtggcggtgc
     5521 gcagatcggg ggcgtagcgc gtcccccgcc cgcccgggcc gtggcagcgc acgcatccct
     5581 tagcgctgaa cagccgctcc ccgttcgccg gattcggcgg ctgcagcagc accacggcgc
     5641 ggtcgcgcag gctcgacgca ctacggatgt aagcctggat gtccgccatc tcacgcccca
     5701 ggaaggtagg gatcgggatg tggtgctccg tctgcgcctc ctgcatcgcc ggcccgtgat
     5761 tccacatccc ctcggcgagc atgatgggcg cgatgtagcg cgcatagccg tcgagcgccg
     5821 gcccgacatg cccgcccgtg ccgccgaccg cgtggcattc cacacagcgc ttttcgcgga
     5881 accactgcgc cccgagatcg gcgtctcccg gcggatcgaa cagcttcacg tagtagatgt
     5941 agctgatgac gtcggccagc tccgactcgg tgaacgtcgg ccagcgcacg ccctggcgcc
     6001 gcaccgtcac gatcatgcgg ggcgtgtggt tccagaactc gcccgcgagc tgctgcatgc
     6061 tgcgccccgc gccgacgacg gcgaagtccg gtcccagcgt gccgccgttt ccccagatgg
     6121 agtgacagtg gacgcagccg cgatccacga agatgcgagc gcccacggtc ggggtccccg
     6181 ggaatacgcc gaccacctgg gcgccgaccc ggccgccgag cagcccgagt agcccgagca
     6241 gcccgagcag cccgcacagg tgtgccgcgt gccgcgtgcg catctcaggg ctccgccgca
     6301 tgaggaagga ccggctctgg cagatagcct cccacgcgct ccgcgaccga gcggaccgat
     6361 tcctcccgtc cttctttgat ctcccagatc ga
//
LOCUS       DBNGZW010000088         2064 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_212028, whole genome shotgun sequence.
ACCESSION   DBNGZW010000088 DBNGZW010000000
VERSION     DBNGZW010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2064)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2064)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2064
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_212028"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1764
                     /locus_tag="AC16JC_02200"
     CDS             <1..1764
                     /locus_tag="AC16JC_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012409569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HIR5163540.1"
                     /translation="PDGNIALTGELDLSHGRDFVLGLAFGDTLHNALTTLFQSLGFPF
                     AEQAKRFVAQWARACRGIEPLAAPSGDGGRLYHVSHSLLLAHEDKTYPGATIASLSIP
                     WGEVKGDDDLGGYHLVWTRDMVSSAIGLLASGHTEQPLRALIYLAVAQRDDGGFYQNF
                     WIDGEPYWSGIQLDEVAFPIILARRLADAQALRDFDPYPLVLRAAGYLIRQGPATPQD
                     RWEENSGYSPSTLAACIAGLTCAAGFAAERGDQGTASFIQEYADFLECHVEDWTVTTD
                     GSLLSGIRRHFIRIHPMDADVPDADEDPNHGLLAVRNRAPGAPWQFPAKEVVDAGFLE
                     LVRYGIRKPGDPLVEDSLQVVDALLKVHTPFGPAWHRYNHDGYGQQENGGPYRGWGKG
                     RAWPLLTGERGHYELAAGRDPRPFIRAMEGFASATGLLPEQVWDEADRPEFFLYLGRP
                     TGSAMPLMWAHAEYVKLLRSARDGAVYDRIPSVAARYQVAGGRVARLLEVWKFGRRVA
                     AVRPGWTLRVQADAPFRLHWTSDEWQQPRDTPSGATAVGIHFVDLPVSAGQRAPIRFT
                     FYWIETGTWEGRDFTVAMRMA"
     gene            complement(1791..>2064)
                     /locus_tag="AC16JC_02205"
     CDS             complement(1791..>2064)
                     /locus_tag="AC16JC_02205"
                     /EC_number="2.3.-.-"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5163541.1"
                     /translation="VVEHDAAAEQFQVRLDGKTAVLQYRRRAGTILFVHTEVPAELQH
                     RGIADALAHAGLEFARAEHLAVVPLCPFVAAYIRRRPEYRLLVETR"
BASE COUNT          301 a          711 c          736 g          316 t
ORIGIN      
        1 ccggacggca acatcgcgct cacgggagag ctcgacctgt cgcacggccg cgacttcgtg
       61 ctggggctcg ccttcggcga cacgctccac aacgcactga cgaccttatt ccagtcgctc
      121 ggcttcccct tcgccgagca ggcgaagcgc ttcgtggcgc agtgggcccg cgcctgccgg
      181 ggtatcgagc cgctggcggc gccgtcgggt gacggggggc ggttgtatca cgtgagtcac
      241 agcctgctgc tggcccacga ggacaagacc tatcccggcg ccacgatcgc ctcgctctcg
      301 atcccttggg gcgaagtgaa aggcgacgac gacctcgggg gatatcacct cgtgtggacg
      361 cgcgacatgg tgagcagcgc gatcggactc ctcgcctccg gccacacgga acagccgctg
      421 cgcgcgctca tctatctcgc cgtcgcgcag cgcgacgacg gcgggttcta ccagaacttc
      481 tggatcgacg gcgagccgta ctggtcgggg atccagctgg acgaggtggc gtttccgatc
      541 atcctcgccc ggcgcctcgc ggacgcgcag gcgctgcggg acttcgatcc ctaccccctg
      601 gtgctgcggg ccgcgggcta cctgatccgc cagggacccg ccacgccgca ggaccggtgg
      661 gaggagaaca gcgggtattc accgtccacg ctggcggcgt gcatcgcggg acttacgtgc
      721 gccgcgggat tcgccgccga gcgcggcgat caggggacgg cgtcattcat ccaggagtac
      781 gcggactttc tcgagtgtca cgtggaggac tggacggtca ccaccgatgg ctcgctcctc
      841 tccgggatcc ggcgacattt catccggatc caccccatgg atgcggatgt ccccgacgca
      901 gacgaggacc cgaaccacgg tttgctggcg gtgcgcaacc gggcgccggg ggcgccctgg
      961 cagttccccg cgaaggaggt cgtcgacgcc ggatttctgg agctggtgcg ctacgggatt
     1021 cgcaagcccg gcgacccgct cgtcgaggac tccctgcagg tggtggatgc cctcctcaag
     1081 gtgcacacgc cgttcggccc tgcgtggcac cgctacaacc acgacggcta cggacagcag
     1141 gagaacggtg ggccgtaccg gggatggggg aagggacgcg cgtggccgct gctcacgggt
     1201 gagcgcgggc attacgagct cgcggcgggc cgcgatccgc gcccgttcat ccgcgcgatg
     1261 gaaggcttcg cgagcgccac cgggctgctc cccgagcagg tgtgggacga ggccgaccgt
     1321 ccggagttct tcctgtatct cggccggccc accggctccg ccatgccgct catgtgggcc
     1381 cacgccgagt acgtgaagct gctccgctcg gcgcgagatg gcgccgtgta cgaccgcatc
     1441 ccgtcggtcg cggcgcgcta ccaggtcgcc ggcgggcgcg tggcgcgact cctcgaggtg
     1501 tggaagttcg gcaggcgggt cgcggcggtg cgccccggct ggaccctccg ggtgcaggcg
     1561 gatgcgccgt tccggctcca ctggaccagc gacgagtggc agcagccgcg cgacacgccc
     1621 tcgggcgcca ccgccgtggg catccacttc gtggaccttc cggtgtccgc cggccagcgg
     1681 gcgccgatcc gtttcacgtt ctactggatc gagacgggga cctgggaagg ccgggacttc
     1741 acggtcgcca tgcgcatggc gtagcacgac gacgtctcct ctcggtatgc ctagcgcgtc
     1801 tccaccagga ggcgatactc gggacggcgt cggatgtacg cggcgacgaa ggggcacagc
     1861 gggacgacgg cgagatgctc tgcccgcgcg aattcgagac cggcgtgcgc cagcgcatcg
     1921 gcgatgcccc ggtgctggag ctccgccggg acctcggtgt gaacgaagag gatcgtcccg
     1981 gctcggcgcc ggtactgcag cacggcggtc ttgccgtcca gccggacctg aaactgctcg
     2041 gcggccgcgt cgtgctccac gacg
//
LOCUS       DBNGZW010000089         4871 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_826130, whole genome shotgun sequence.
ACCESSION   DBNGZW010000089 DBNGZW010000000
VERSION     DBNGZW010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4871)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4871)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4871
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_826130"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2131)
                     /locus_tag="AC16JC_02210"
     CDS             complement(<1..2131)
                     /locus_tag="AC16JC_02210"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013030646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HIR5163542.1"
                     /translation="MRLSLRFLVPLLLVMAAFAYAAVPLADRLTLRWFMRDMDSRSSL
                     IANSVHESLQDLVGTDNDARVLQLFTRITQDERIYAMGFCASPRAGVLATVTLPDVIH
                     CDSLAGFSDPEGHLLRSGQGPLLVSVRALPLKAAPEGRLVLVHDMSFVERRSAETRKY
                     LFYFFLGLGFIVSLITVVVAQLSWRGWVQGLRALLRGEGLFRPAEKSERAELRPIASD
                     LRALIHDLEAEHRSRDDQQMPWTPDTLRGILRRELRDQEVIVVSNREPYIHRRHGDAI
                     VVQRPASGLVTALEPVMRACSGTWIAHGSGSADREVVDDRDRVGVPRDNPAYQLRRVW
                     LTPEQEAGYYYGFANEGLWPLCHIAHVRPTFRTADWEQYVNVNHLFARAVVQESKSDD
                     PIVLVQDYHFALLPRMIKEALSAATIITFWHIPWPNPEAFGICPWREELLDGLLGSSI
                     LGFHTQFHCNNFVDTVDRLLEARVDRETFTVSYRGKLTAVKRYPISVEWPPAAELLDK
                     PVERCRLDVRERHRLPPDHAIGVGIDRLDYTKGIEERFRAVERLLELRPEWVGRFTFI
                     QVAAPTRASIEQYQDYAARVRTMAARSNARFPDAAAPPILRLVEHQEPVDVYEYYRAA
                     DLCMVSSLHDGMNLVAKEFVSARNDDRGVLILSQFTGAARELPEAIIVNPYDTEQCAT
                     ALHLALEMPVREQRARMRLMRGLVQEFN"
     gene            complement(2170..3453)
                     /locus_tag="AC16JC_02215"
     CDS             complement(2170..3453)
                     /locus_tag="AC16JC_02215"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007432756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HIR5163543.1"
                     /translation="MPDRLAHLSEVDPAIHDLIKREVARQEHGLELIASENFVSVAVL
                     EAMGTALTNKYAEGVPRKRYYGGCEVVDEVEQLAIDRAKALFGAAHANVQPHSGAQAN
                     MAAYLAVLKPGETLMGLALPHGGHLTHGSPVNFSGVLFRAVAYGVREDTGIIDYDEVR
                     ATALAERPQLIVAGGSAYARIIDFEALRAIADESGAKFLVDMSHFAGLVAGGVHPSPV
                     PHAQIVTTTTHKTLRGPRGGMILCTAELAKAVDKWVFPGTQGGPLEHIIAAKAVALGE
                     AARPDFRTYAAQVVRNAQALAQALLGRGYAIVSGGTDTHLMLVDLRPKALTGKDAEQL
                     LDRAGITVNKNTIPGDPQSPFVTSGIRLGTPAVTTRGFREPEMARVAELIDEVLTRKD
                     EATLARVKDAVRALTDAFPLYAGRQGEVGAASRGQ"
     gene            complement(3495..3932)
                     /locus_tag="AC16JC_02220"
     CDS             complement(3495..3932)
                     /locus_tag="AC16JC_02220"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HIR5163544.1"
                     /translation="MSEQVYVGADHAGFELKQKLVAELRRLGYEPVDVGPASLDPADD
                     FPDYARPVAEAVSHGAARRGVLTCGTGLGMAYVANRYPHVRAAVVWNPEVAELSRRHN
                     DANVLVLPSRFLKDDDSLDILRRWLATPFEGGRHQRRVEKIDK"
     gene            complement(3925..>4871)
                     /locus_tag="AC16JC_02225"
     CDS             complement(3925..>4871)
                     /locus_tag="AC16JC_02225"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009373861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Leu/Phe/Val dehydrogenase"
                     /protein_id="HIR5163545.1"
                     /translation="PALGGTRFWHYQDDLAALVDALRLARGMTYKAAVAGINLGGGKS
                     VIIGDNKITRREPIFRAHGRHVESLNGRYITAEDVGTSTADMEFIRAETEHVTGLLGK
                     SGDPSPVTAYGVYRGIKACAQVRWGSDSLTGKTIVVQGCGHVGYSLCKLLFDEGATLF
                     VADIDPQRLKAAVQDFKAVALSEDEVYRQAADIFAPCALGAVINDKTLPQLKVAIVAG
                     AANNQLAEDRHGEEIEARGILYAPDYVINGGGLINVNAELHGWSLERARNKAGEIYDT
                     ILRVFDIAKEEGIPSYQAADRLAEQRIAAVAGVRQNFV"
BASE COUNT          727 a         1731 c         1650 g          763 t
ORIGIN      
        1 cgttgaattc ctgcaccagg ccgcgcatga gccgcatgcg cgcgcgctgc tcgcgcaccg
       61 gcatctcgag ggcgaggtgc agcgccgtcg cgcactgctc ggtgtcgtag ggattgacga
      121 tgatcgcttc cggcagctcg cgcgcggcgc cggtgaactg gctgagaatg agcacgccgc
      181 ggtcgtcgtt gcgggcggac acgaactcct tggcgacgag gttcatgccg tcgtgcaggc
      241 tcgagaccat gcacaggtcg gccgcgcggt agtactcgta gacgtccacc ggctcctggt
      301 gctccaccag gcgcagaatc ggcggcgcgg cggcgtccgg gaagcgcgcg ttgctgcggg
      361 cggccatggt gcgcacgcga gccgcgtagt cctggtactg ctcgatgctg gcgcgcgtcg
      421 gcgcggccac ctggatgaac gtgaaccgcc ccacccactc cggccgcagc tccagcaggc
      481 gttccacggc gcggaaccgc tcctcgatgc ccttggtgta gtcgagacgg tcgatcccca
      541 cgccgatggc gtggtcgggg ggcagccggt gacgctcccg cacgtccagc cggcaccgct
      601 ccaccggctt gtccagcagc tcggccgcgg ggggccattc gaccgaaatc ggataccgct
      661 tcacggcggt cagcttgccg cggtaggaca cggtgaacgt ttcgcggtcc acccgcgcct
      721 ccagcagccg gtccaccgtg tccacgaagt tgttgcagtg aaactgcgtg tgaaagccga
      781 ggatgctgct gcccagcagg ccgtcgagca gctcctcgcg ccaggggcag atgccgaacg
      841 cctcggggtt gggccagggg atgtgccaga acgtgatgat cgtggcggcg gacagcgcct
      901 ccttgatcat ccgcggcaag agcgcgaagt gatagtcctg gaccagcacg atggggtcgt
      961 cggacttgga ctcctgcacc accgcccggg cgaacaggtg gttgacgttc acgtactgct
     1021 cccagtccgc ggtccggaac gtgggccgca cgtgggcgat gtggcacagc ggccagagcc
     1081 cttcgttggc gaatccgtag tagtagccgg cctcctgctc gggggtcagc cagacgcggc
     1141 gcagctgata ggccgggttg tctcgcggca cgccgacgcg gtcccggtcg tccaccacct
     1201 cgcggtcggc cgagcccgag ccgtgcgcga tccaggtccc cgagcaggcg cgcatcaccg
     1261 gctccagcgc ggtgacgaga ccgctcgccg gccgttgcac gacgatcgcg tcgccgtggc
     1321 gtctgtggat gtagggctcg cgattggaga cgacgatcac ctcctggtcc cgcagctccc
     1381 gccgcaggat cccgcgcagc gtgtccggcg tccagggcat ctgctggtcg tcgcgcgaac
     1441 gatgctccgc ttcgagatcg tggatcagcg cgcgcaggtc gctcgcgatg ggccgcagct
     1501 cggcgcgctc ggacttctcg gcgggccgga acagcccttc gccgcgcagc agcgcccgca
     1561 gcccctggac ccagccgcgc cagctcagct gcgccaccac aacggtaatg agcgaaacga
     1621 tgaagccgag cccgagaaag aaatagaaga ggtacttccg cgtctcggcg ctgcggcgct
     1681 cgacgaagct catgtcgtgc accagcacga gccggccctc gggcgcggcc ttcagcggca
     1741 gcgcgcgcac cgacaccagc agcggcccct gcccgctccg cagcaggtgc ccctccggat
     1801 ccgagaagcc ggcgagactg tcgcaatgga tcacgtcggg cagcgtcacg gtggcgagca
     1861 ccccggcccg cggcgaggcg cagaacccca tcgcgtagat gcgctcgtcc tgggtgatcc
     1921 gcgtgaacag ctgcagcacc cgggcgtcgt tgtcggtccc gacgagatcc tgcaacgact
     1981 cgtgtaccga gttggcgatc agggaagacc ggctgtccat gtctcgcatg aaccagcgca
     2041 gcgtcaaccg gtccgccagc ggcaccgccg cataggcgaa ggccgccatc accaggagca
     2101 gcggcaccag gaatcgcagg gagagacgca tcgagacctg cctacccctt ccccgacccg
     2161 acgttcacct cattgccccc ggctggccgc gcccacttct ccttgccttc cggcgtagag
     2221 cggaaatgcg tcggtcagcg cccgcaccgc gtccttcacc cgcgcgagcg tcgcctcgtc
     2281 cttgcgcgtc aggacctcgt cgatcagctc ggccacgcgc gccatctcgg gctcacggaa
     2341 gccgcgcgtc gtcacggccg gcgttcccag ccggatgccg ctcgtgacga aaggcgactg
     2401 cggatccccg gggatggtgt tcttgttcac cgtgatcccg gcgcggtcga gcagctgctc
     2461 ggcatccttc ccggtgagcg ccttgggacg caggtccacg agcatgaggt gcgtgtcggt
     2521 cccgcccgac acgatggcat agccgcgccc cagcagcgcc tgcgccagcg cctgtgcgtt
     2581 gcgtaccacc tgggccgcgt acgtcctgaa gtcgggacgg gcggcctcgc ccaacgccac
     2641 ggccttggcg gcgatgatgt gctcgagcgg cccgccctgc gttcccggaa acacccactt
     2701 gtccaccgcc ttggccagct cggcggtaca gagaatcatc ccgccgcgcg ggccgcgcag
     2761 cgtcttgtgg gtcgtggtgg tgacgatctg ggcgtggggc acgggcgagg gatgcacgcc
     2821 gcccgccacc agccccgcga agtgcgacat gtccaccagg aatttcgcgc cgctctcgtc
     2881 ggcgatcgcg cgcagcgcct cgaagtcgat gatgcgcgcg taggcgctcc cgcccgccac
     2941 gatcagctgc gggcgctcgg ccagcgccgt cgcgcgcacc tcgtcgtagt cgatgatccc
     3001 cgtgtcttcg cgcacgccgt aggccacggc gcgaaacagc acgccggaga agttcacggg
     3061 cgagccgtgg gtcaggtgcc cgccgtgcgg cagcgcgagt cccatcagcg tctcgcccgg
     3121 cttcagcacc gccaggtagg ccgccatgtt ggcctgggcg cccgagtggg gctggacgtt
     3181 ggcgtgcgcc gcgccgaaca gcgccttggc gcggtcgatc gcgagctgct ccacctcgtc
     3241 caccacctcg caaccgccgt agtagcgctt ccgcggcact ccctcggcgt acttgttcgt
     3301 gagggcggtg cccatcgcct cgagcaccgc gacggagacg aagttctcgc tggcgatcag
     3361 ctccagtccg tgctcctgac gcgcgacctc gcgcttgatc aggtcgtgaa tcgcagggtc
     3421 cacctcggag agatgcgcca gccgatcagg catgtgcatt cacccgatac ccgacacctg
     3481 atacctggta tctgtcattt atcgatcttc tccacacggc gctgatggcg gccgccctcg
     3541 aacggggtgg cgagccagcg gcgcaggatg tccaggctgt cgtcgtcctt gaggaaccgg
     3601 gacggcaaca ccagcacgtt cgcgtcgttg tgccggcgcg acaactcggc gacctcggga
     3661 ttccacacca ccgccgcccg gacatgcggg tagcggttgg cgacgtaggc catgccgagg
     3721 cccgtcccgc aggtcaggac gccgcgccgg gccgccccgt gactcacggc ctccgcgacg
     3781 ggccgggcgt agtccgggaa gtcgtcggcg ggatccaggg atgccgggcc cacgtcgacg
     3841 ggctcgtagc ccaggcggcg cagctccgcg accagcttct gcttgagctc gaagccggca
     3901 tgatcggcgc cgacgtagac ctgctcagac aaagttctgc ctgacgccgg ccacggcggc
     3961 gatgcgctgc tcggccagcc ggtcggcggc ctggtagctc gggatcccct cctccttcgc
     4021 gatgtcgaac acgcgcagga tggtgtcgta gatctcgccc gccttgttgc gcgcccgctc
     4081 gagcgaccag ccgtgcagct ccgcgttcac gttgatcaag ccgccgccgt tgatcacgta
     4141 atcgggagcg tagaggatcc ccctggcttc gatctcctct ccgtgccggt cctcggcgag
     4201 ctggttgttg gccgcgccgg ccacgatggc caccttgagc tgcggcagcg tcttatcgtt
     4261 gatcacggcg ccgagcgcgc agggcgcgaa gatgtcggcg gcctggcggt agacctcgtc
     4321 ctcggacagg gcgacggcct tgaagtcctg caccgcggcc ttcaggcgct ggggatcgat
     4381 gtcggcgacg aacagcgtgg ccccttcatc gaacagcagc ttgcagaggg agtagcccac
     4441 gtggccgcac ccctgcacca cgatcgtctt ccccgtgagg ctgtccgacc cccagcgcac
     4501 ctgggcgcag gccttgatgc cgcggtacac accgtaggcg gtcactggcg agggatcgcc
     4561 cgacttgccg aggagaccgg tgacgtgctc ggtctccgcc cggatgaact ccatgtccgc
     4621 ggtggaggtg cccacgtcct cggcggtgat gtagcggccg ttcaacgatt ccacgtgccg
     4681 cccgtgggcg cggaagatcg gctcgcggcg cgtgatcttg ttgtcgccga tgatcacgct
     4741 cttgccgccg ccgaggttga tccccgcgac cgccgccttg taggtcatgc cgcgcgccag
     4801 ccgcagcgcg tccaccagcg ccgcgagatc gtcctgatag tgccagaagc gcgtgccgcc
     4861 gagcgcgggc c
//
LOCUS       DBNGZW010000090         3228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_826221, whole genome shotgun sequence.
ACCESSION   DBNGZW010000090 DBNGZW010000000
VERSION     DBNGZW010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_826221"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..683)
                     /locus_tag="AC16JC_02230"
     CDS             complement(204..683)
                     /locus_tag="AC16JC_02230"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR5163546.1"
                     /translation="MKRRLKPDVSGGSVVSDINITPMVDVLLCLLILQMVIQPGLQKG
                     LDLQLPLSERTEMAPGTVARDQIVLRVTAGPAYALNQQAVPVADLERTLRDVFRDRPR
                     KVLFVDGAEDISYDEVIGVVDAARGAGLAVVGLVPRPDRRPDPAPAAGSPVFPASQF"
     gene            complement(680..1159)
                     /locus_tag="AC16JC_02235"
     CDS             complement(680..1159)
                     /locus_tag="AC16JC_02235"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ExbD/TolR family protein"
                     /protein_id="HIR5163547.1"
                     /translation="MAFSAKRTAGPSNLPVLRALGGEHTVAELNITPMIDVMLALLII
                     FMVVTPVLTEYAATLPVARHPVPEALDDAVTLAIDRNGTFLVGSDTIPAARLTERLAR
                     IYAARPGDHLLYLRADRGVPYRLVLDAVDAARAAGVRKVGAISFPVMSGDSGGVRSP"
     gene            complement(1159..1845)
                     /locus_tag="AC16JC_02240"
     CDS             complement(1159..1845)
                     /locus_tag="AC16JC_02240"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR5163548.1"
                     /translation="MTLVELVGRMGVIPWLVIALLAALNVLTLTVAAVKWHRLRALRR
                     STRAFAPAFALALEEDRIDDAVQLARAHGSSHMAKVLGDALERAAPLLDDPDLAPRAV
                     ESAERMVLRSQIRLATELKHRLSALATIGATAPFVGLLGTVVGITNSFIGIARSGGGG
                     IEAVAGGVGEALITTAVGLVVAIPALWLYNYFIARLEELFAELAYVSDELIDWLSLRR
                     AGVVMKYEMA"
     gene            complement(1842..2543)
                     /locus_tag="AC16JC_02245"
     CDS             complement(1842..2543)
                     /locus_tag="AC16JC_02245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR5163549.1"
                     /translation="MLSARPAAEGPPETVTYLDIPPPTVASSPRALLPPRPPVVRQGL
                     RIPAVLAQVAQPGKLAGFQELVTPARLAGIPPVDPAQETVSALDFSGRGVAGGVAGGQ
                     KPEEVPPPTDSTPLAPEGAAYTVAAVGEPPVLLNRAELPAIMQSLYPPLLRVAGLGGR
                     VRVEFVVDRTGRVDRRSVRTLESAHPLLSSATQTALEQFRFRPGSVSSGDQKRLVPVL
                     VAMTIEWTLRDGGSR"
     gene            2958..3140
                     /locus_tag="AC16JC_02250"
     CDS             2958..3140
                     /locus_tag="AC16JC_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163550.1"
                     /translation="MAEATRKNQERIIANEKRILQNQRRLLRILDNEQKIIRNQQAIL
                     KNQKKILGNQSKILAK"
BASE COUNT          566 a         1112 c         1091 g          459 t
ORIGIN      
        1 ccacacacgg gcgcgcggcg cgcgcagcac aatccgcttc tcgatacggt ccgtgctcga
       61 cgtctcctgg gatgttgcgg atttggcagt catgaggtgg tttcccctgt tgctgaggtc
      121 cgtgtgggct tccgaattat gcaacataca tgttgcatat cataccacgg gccggcccta
      181 catgcaaccc ccatgttgcg cgttcagaat tgggacgctg ggaagaccgg gctccccgcg
      241 gccggggccg gatccggacg ccgatcaggg cgcggcacca ggccgacgac cgccagaccg
      301 gcgccgcgcg cggcgtccac cacgccgatc acttcgtcgt acgagatgtc ctcggcccca
      361 tccacgaaga ggacctttcg gggccggtcg cggaacacat cccgcagcgt gcgctcgagg
      421 tccgcgaccg gcacggcctg ctggttgagc gcataggcgg gtcccgccgt cacgcgcagc
      481 acgatctgat cgcgggcgac cgtgccgggc gccatttccg tgcgctcgga cagcggcaac
      541 tggaggtcca gacccttctg cagaccgggc tggatcacca tctggagaat gagcaggcac
      601 agcagcacgt ccaccatcgg cgtgatgttg atgtccgaca cgacggaccc gccgctcaca
      661 tcgggtttca ggcgccgctt catggcgagc ggactccgcc gctgtcaccc gacatcaccg
      721 ggaagctgat ggcgccgacc ttccgcacgc ccgcggcgcg cgcggcgtct accgcgtcga
      781 gcacgaggcg atacggcacg ccccggtcgg cccgcaggta gagcagatgg tcgcccggac
      841 gggccgcgta gatccgcgcc agtcgctccg tcaggcgggc ggccgggatg gtgtcgctgc
      901 ccacgaggaa cgtcccgttg cggtcgatgg ccagcgtgac ggcatcgtcc agcgcctcgg
      961 gcacggggtg gcgcgccacc ggtagcgtgg cggcgtactc ggtgagcacg ggtgtcacga
     1021 ccatgaagat gatcagcagc gccagcatga cgtcgatcat cggggtgatg ttgagctcgg
     1081 cgacggtgtg ttccccaccg agggcgcgca gcaccggaag gttggacgga ccggcggtcc
     1141 gtttggccga gaatgccatt acgccatctc gtacttcatg acgaccccgg cccggcgcag
     1201 cgacagccag tcgatcagct cgtcggagac gtacgccagc tcggcgaaca gctcttccag
     1261 gcgggcgatg aagtagttgt agagccagag cgcggggatc gccaccacga ggcccaccgc
     1321 ggtcgtgatc agcgcctcac ccacgccgcc ggccacggcc tcgataccgc cgccgcccga
     1381 gcgtgcgatc ccgatgaacg agttggtgat cccgacgacc gtcccgagca ggcccacgaa
     1441 gggggcggtc gcaccgatgg tcgcgagcgc cgagaggcgg tgtttgagct cggtggccag
     1501 gcggatctga ctccgcagga ccatgcgctc ggcggattcg accgcacgcg gcgcgagatc
     1561 ggggtcgtcg agcaacggcg ccgcccgctc cagggcgtcg ccgaggacct tggccatgtg
     1621 ggacgagccg tgggcgcggg ccagctggac cgcgtcgtcg atccgatcct cttcgagcgc
     1681 cagggcgaat gccggcgcga acgcgcgcgt cgatcgcctc agggcccgca gacggtgcca
     1741 tttcacggcg gcgaccgtaa gcgtcagcac gttgagcgcc gccagcaggg cgatcaccag
     1801 ccatgggatc actcccatgc ggccgacaag ttcgaccagc gtcatctgga gcctccatcc
     1861 ctcagggtcc actcgatcgt catcgccacg aggaccggta ccaatcgctt ctggtcgccg
     1921 ctcgacacgc tgccggggcg gaagcgaaac tgctcgagcg cggtctgcgt cgccgagctg
     1981 agcagcggat gagccgattc gagggtgcgc accgagcgcc ggtccactcg accggtccgg
     2041 tccaccacga actcgacacg cacgcgcccg cccaggcccg ccacgcgcaa gagcggggga
     2101 tacaggctct gcatgatcgc agggagctcg gcccgattca ggagcaccgg cggctccccg
     2161 acggccgcca ccgtgtacgc cgctccttcc ggagcgagcg gcgtcgagtc ggtcggcggg
     2221 gggacctcct cgggcttctg gccgccggcc accccgcccg ccacccctcg ccccgagaag
     2281 tccagcgcgc tcaccgtttc ctgggccggg tccaccggcg ggatcccggc cagccgcgcc
     2341 ggcgtcacca gctcctggaa gccggcgagc ttcccgggct gcgcgacctg cgccaggacg
     2401 gcggggatgc gtagcccctg acgcaccacg ggaggccgag gcggcagcag cgcgcgcggt
     2461 gacgacgcga cggtcggagg cggaatgtcc agatacgtca cggtctcggg aggaccctcg
     2521 gcagccggtc gggcggaaag catgaacatc actgccagaa gaacggcaag atgcccggcc
     2581 gtcgttgccg ccccgggggg tagctcctct cgccaggacg aggcccgggg gcccgacgcg
     2641 aacagctgcc cgaagagccg aatgcgcacg catcgttggc tctccagcat cgccggcgct
     2701 gcttccacac cctgcatccg ccccccgatg tcatgtcaaa caaggggtta cgtcctggga
     2761 aatacctacc gctgtggcga caaaggcgag atgaatgcgc ggtgagggtt gtgtcattct
     2821 ggccgggcgc gggcgtacgc tccgagattc cctctcgtat tgccgaggtc cacggcgagg
     2881 tataccgtat cggcctgacc gggccgacgg gaacgcggcc tcacccgtga attccgaacc
     2941 acaacgagga gtgcgcaatg gcggaagcga cccgcaagaa ccaggagagg atcatcgcga
     3001 acgaaaagag gatcctgcag aaccaacgcc ggttgttgcg gatcctcgat aatgagcaga
     3061 agatcattcg gaaccagcag gctatcctga agaaccaaaa gaagatcctg ggcaatcaga
     3121 gcaagatcct cgccaaatag gcgccctgcg gctagcgcgc gtcgatgacg aaatccaccg
     3181 cgctcgcgta ggcgcgccaa cgcgcgatct cctcacggag cgcctctt
//
LOCUS       DBNGZW010000091         7579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_963423, whole genome shotgun sequence.
ACCESSION   DBNGZW010000091 DBNGZW010000000
VERSION     DBNGZW010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7579
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_963423"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..387)
                     /locus_tag="AC16JC_02255"
     CDS             complement(<1..387)
                     /locus_tag="AC16JC_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HIR5163551.1"
                     /translation="MSEFMLQQTQVSRVTEYYPRFLDRFGSIEALARARGSAVREAWE
                     GLGYYARARNLHRLAKEVSGAGGQVPDDPAELIRLPGVGRYTAGAVACFAYEKPVAAV
                     DTNVARVIKRVFFGDEGRGTRDGLRRR"
     gene            677..2038
                     /locus_tag="AC16JC_02260"
     CDS             677..2038
                     /locus_tag="AC16JC_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR5163552.1"
                     /translation="MRAPITGPMVAAVLAALPPAAFAQTSLPPDSAVRAILTERLPGQ
                     RGGGFVVGLLDADGARRVVNVGDPADGVFEIGSITKVFTTAVLADMVARGQVRLDDPV
                     ARLLPASVKVPSRGGRQITLLDLATQSSGLPRLPTNFAPQDSTNPYADYGEQQLYAFL
                     AGYERPRDPGATYEYSNLGMGLLGHALARRAGMSYERLVTRRVLAPLGMNETAITLSP
                     ALRARLVPGHGDTGQVVANWDLTALAGAGALRSTVRDMLTFLAANLDSSRGPLARPLH
                     ETHVSRHEAGSPNMTIGLAWHILARRGGGTIVWHNGGTGGYRSFIGFDPARGLGVVLL
                     TNSVIGADDIGFHLLDASLPLRPPPKPRNEVTVDSIVLGRYVGGYELVPAFHIVVTQE
                     GKALFIQATGQSKFPIFAESETDFFLKVVDAQLTFVRDSTGVVTGLVLHQNGRDTPGR
                     RLP"
     gene            2035..2835
                     /locus_tag="AC16JC_02265"
     CDS             2035..2835
                     /locus_tag="AC16JC_02265"
                     /inference="COORDINATES: protein motif:HMM:NF014460.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR5163553.1"
                     /translation="MRLSGVERAIDLRRYAGRWFLHAAGHAGRLFYLVMDMFRGLPEW
                     RTYAPRTAEQANLIGYGSLFIVILISGFAGGVTALQTGYQFTGAYPLYIAGGLIAETV
                     ILELGPVMTGLILAGRIGARYAAELGTMRVTEQIDALESLGRSPVTHLLLPRCLAGIL
                     VIPALVMISNATGFLVGWFAAKQSLGITNAEFTYGARYFFRSFDLWYSGIKAVCFAVA
                     VTLIPCYIGFNTKQGAEGVGRATTQAVVAASVTILLLDVVLAQVLLGK"
     gene            2843..3604
                     /locus_tag="AC16JC_02270"
     CDS             2843..3604
                     /locus_tag="AC16JC_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5163554.1"
                     /translation="MIELKHVSKRFFENVVLDDVSLEVRRGETLALLGPSGVGKSVLL
                     KHIIGLIRPDSGDVIVDGLNVAHLGRKELSQLRTRIGYVFQNGALFDSMDVFENIRLG
                     ITDETAYRDLKFAHHRVQECLKLVNLQPDVAKKMPAELSGGMRKRVGIARAIAGKPTY
                     LLYDEPTSGLDPVNSDVIDGLIERLQQELGVTSVVVSHDVRGSFKVADRIALLWQAKI
                     RAAGTAEEILASQDPAVQQFLERDLEMTLLQGGKS"
     gene            3605..4492
                     /locus_tag="AC16JC_02275"
     CDS             3605..4492
                     /locus_tag="AC16JC_02275"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR5163555.1"
                     /translation="MDLTYKQEVGVGALVLAGLTLFVVGLFWFSGRSIRHKGVYAHVV
                     FTNVAGLKEGDPVLVSGVKVGRVSKVSLERVGRVTVTLELSGEERPRTDASATISSLD
                     FFGAKFVDYIPGSKDDFLPATSVIRGTKSQELTDIASGVATRANELLGNATGLVSDQL
                     ADDIHNTLVATQRGMVALTEVTKGPLVTQSTNSLAALERVMARLDTLLGSANVKGAGL
                     RFDTLSANLQHLSGELSQSTRALNDLLGKVNRGEGTLGKLASDTLLYGNLNKTLDALT
                     KLMNDLRERPGRYLTVKVF"
     gene            4597..5529
                     /gene="rnz"
                     /locus_tag="AC16JC_02280"
     CDS             4597..5529
                     /gene="rnz"
                     /locus_tag="AC16JC_02280"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - RNA endonuclease activity
                     producing 5'-phosphomonoesters, hydrolytic mechanism
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HIR5163556.1"
                     /translation="MLSVTFLGTSAARPTIERNVSSLALMREGETLLFDCGEGTQRQM
                     MRYGVSFALDDIFFTHFHGDHFLGVIGLIRTLGLQGREEPLRLWGPRGAKQVLKSAMM
                     LGVERLPFDVQIEEVKAGDAIGDGGRGTRDGYTLRVFATEHGGGSVGYAMVENERYGR
                     FDPDKARAAGIPEGPLWGKLQRGESVVLADGRQLTADSVVGPRRPGRKVVITGDTRPC
                     ASVVDAATGADLLVHEATFGEEEKERSRETMHSTAREAAQVALAAQVRKLVLSHLSAR
                     YSITAEDLVKEAREVFVNVVAAKDGMTVDVPFAE"
     gene            complement(5550..5978)
                     /locus_tag="AC16JC_02285"
     CDS             complement(5550..5978)
                     /locus_tag="AC16JC_02285"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5163557.1"
                     /translation="MRPVTFTLTTTVPAPPEQVFAAITDPHRMALWLPSARAVEAPEG
                     ALQKGSRIRIVYDARDTEIEVVDMNPPQVFGWAERVGRRHWRTFFRLEFAGSSTKLTV
                     TQVWNPPSLLAWLKVKLRPSRNVPARLSTMVQNLRTVMAR"
     gene            6120..6503
                     /locus_tag="AC16JC_02290"
     CDS             6120..6503
                     /locus_tag="AC16JC_02290"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5163558.1"
                     /translation="MTQPEADVLSAERSWFDGLLAARVPQLEALLTSDFQIIDVSSGA
                     EADRAAVLGAIDAGQLRFEAIDVLESRTRRYGDTVLVTGRTRMRGQFQGRPFGAHSRY
                     THVFVRQEGRWRLASAQGTPIVTTP"
     gene            6666..6738
                     /locus_tag="AC16JC_02295"
     tRNA            6666..6738
                     /locus_tag="AC16JC_02295"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6699..6701,aa:Ala,seq:cgc)
     gene            6750..6822
                     /locus_tag="AC16JC_02300"
     tRNA            6750..6822
                     /locus_tag="AC16JC_02300"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6783..6785,aa:Gln,seq:ctg)
     gene            complement(6913..>7579)
                     /locus_tag="AC16JC_02305"
     CDS             complement(6913..>7579)
                     /locus_tag="AC16JC_02305"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR5163559.1"
                     /translation="HRGAPYLQRNRLAGWHPPREQDTRRPLIADATITALVAVAPRVH
                     PRLTLLILLLETTGRRLSSVLGLRWDDLDFARGVIRWRGVLDKKRRTQDAPMPTRAAR
                     ALAAERAAHPAIGGALLFPSRRDPGVPLSRHAAADWLKRAYRYAGLSRPPGGLWHPFR
                     RKWATERKHHPVRDLAEAGGWKDVQTLLTCYVQADLDTMREVVEQPQRRRASPGVSSL
                     LTS"
BASE COUNT         1211 a         2502 c         2622 g         1244 t
ORIGIN      
        1 tctccgtcgt aacccgtccc tcgtccctcg tccctcgtcc ccaaagaaga ctctctttat
       61 gacgcgcgcc acgttcgtat ccaccgccgc caccggcttc tcgtaggcga agcacgccac
      121 cgcaccggcc gtgtaccgcc ccacgcccgg gagcctgatc aattccgcgg ggtcatccgg
      181 cacctgtccc ccggcacctg acacctcttt tgccagccgg tgcaggttcc gcgcccgcgc
      241 gtagtatccc agtccctccc acgcttcgcg cacggccgac cccctcgccc gggccagcgc
      301 ctcgatgctc ccgaaccggt cgaggaagcg cggataatac tccgtcaccc ggctcacctg
      361 ggtctgctgc agcatgaact cgctcaccag cacgcggtag ggatcacgcg tgcggcgcca
      421 cggcaggtct cgaccgtgtc gctcgaacca ggagaggagg cgggctcgga accgccgcgt
      481 gagggcaggg cgcggcgtat gtgcgtggtg cgtggtgcgt ggtatgttcc ggccctccct
      541 gtggcgacgg caacgagtac gagtgtcgta accgcaactc caacgcggac cggcaaatac
      601 cacccaccac gcaccacgga cagctttcgg ggtgggggtg gacgtggcgg gcccgggtcc
      661 ccaagataca cccacgatga gagccccgat caccggtccg atggtcgccg cagtcctcgc
      721 cgcgctgccg ccggccgcgt tcgcccagac ctccctcccg cccgactcgg cggtccgggc
      781 gatcctgacc gagcgcctgc cgggtcagcg gggagggggc ttcgtcgtgg gcctgctcga
      841 tgccgacggt gcgcgccgcg tggtgaacgt cggcgaccct gccgacgggg tgttcgagat
      901 tggatcgatc accaaggtgt tcaccacggc cgtcctggcc gacatggtgg cgcgcggcca
      961 ggtgcggctc gacgatcccg tggcgcggct gctccccgcc tcggtgaagg tgccgagccg
     1021 cggtgggcgc cagatcacgc tgctcgacct cgccacccag tcgtcggggc tgccgcgcct
     1081 gcccacgaac ttcgcccccc aggactcgac caacccgtac gccgactacg gtgagcagca
     1141 gctgtacgcg ttcctggcgg gatacgagcg gccgcgcgac cccggggcga cctacgagta
     1201 ctcgaacctg gggatggggc tcctgggaca cgcgctcgcg cgccgggcgg ggatgtccta
     1261 cgagcggctc gtgacccggc gcgtgctcgc gccgctcggg atgaacgaga cggccatcac
     1321 gctgtcgccc gcgctgcggg cgcggctcgt tccgggccac ggcgacaccg gtcaggtcgt
     1381 ggcgaactgg gatctcacgg cgctggccgg cgccggcgcg ttgcgctcca cggtgcgcga
     1441 catgctcacg ttcctggccg cgaatctcga ctcgagtcgg gggcccctgg cccggccgct
     1501 gcacgaaacc cacgtctccc gccacgaggc cggaagtccc aacatgacga tcggcctcgc
     1561 ctggcacatt ctcgcgcgcc gggggggcgg taccatcgtg tggcacaacg gcggaacggg
     1621 gggctaccgg tcgttcatcg gcttcgatcc ggctagaggg ctcggggtgg tgctgctcac
     1681 caattcggtg atcggcgcgg acgacatcgg ctttcacctg ctcgacgcct cgctgccgct
     1741 gcgcccgccg ccgaaaccgc gcaatgaggt gacggtcgat tccatcgtcc tgggccgcta
     1801 cgtggggggg tacgagctgg tcccggcgtt tcacatcgtc gtcacgcagg aaggaaaggc
     1861 cctcttcatc caggcgacgg ggcagtccaa gttcccgatc ttcgccgagt cggagacgga
     1921 cttcttcctg aaggtggtgg acgcgcagct taccttcgtg cgtgattcca ccggggtggt
     1981 gacggggttg gtgttgcacc agaacgggcg tgacaccccg ggacggcgcc tgccgtgaga
     2041 ctctccggcg tcgagcgggc catcgacctg cggcgctacg cggggcgatg gttcctgcac
     2101 gccgccggcc acgccggccg gctcttctac ctggtgatgg acatgttccg cgggctgccg
     2161 gagtggcgca cctatgcgcc gcggaccgcc gagcaggcca acctgatcgg ttacggcagt
     2221 ctcttcatcg tgatcctgat ctccggtttc gcgggcggcg tgacggccct gcagaccggc
     2281 taccagttca ccggcgccta cccgctctac atcgcgggcg gcctgattgc ggagacggtg
     2341 atcctggaac tggggccggt gatgacgggg ctgatcctgg ccggtcgcat cggcgcgcgc
     2401 tacgccgcgg agctcggcac gatgcgcgtg accgagcaga tcgatgcgct cgagagcctg
     2461 ggccgctcgc cggtgacgca cctgctcctg ccgcgctgcc tggcggggat cctggtgatt
     2521 cccgcgctgg tgatgatctc caacgccacg ggcttcctgg tggggtggtt cgcggccaaa
     2581 caatcgctcg gcatcacgaa cgccgaattc acctacgggg cccggtattt cttccggtcg
     2641 ttcgatctgt ggtattccgg aatcaaggcg gtatgcttcg cggtcgcggt gaccctgatc
     2701 ccgtgctaca tcggattcaa caccaagcag ggcgcggaag gggtgggccg cgcgacgacc
     2761 caggccgtcg tggcggcctc ggtcacgatc ctgttgctcg atgtcgtcct cgcccaggtg
     2821 ctgctcggca aatgaacgga tcatgataga actcaagcac gtgtcgaagc gtttcttcga
     2881 gaacgtcgtg ctggacgacg tgagcctcga ggtgcgccgc ggcgagacgc tggcgttgct
     2941 cggcccctcg ggcgtaggca aaagcgtgct gttgaagcac atcatcgggc tgatccgccc
     3001 cgacagcggg gacgtcatcg tggacggcct caacgtggcg cacctgggtc gcaaggagct
     3061 gtcgcagctg cgcacccgca tcggctacgt gttccagaac ggcgcgctgt tcgactcgat
     3121 ggacgtgttc gagaacatcc gcctcggcat caccgacgag acggcctacc gcgacctgaa
     3181 gttcgcgcat caccgggtgc aggagtgcct gaagctcgtc aacctgcagc ccgacgtcgc
     3241 gaagaagatg ccggccgagc tctcgggcgg gatgcgcaag cgggtgggca tcgcgcgggc
     3301 catcgccggg aagccgacct atctgctgta cgacgagccg acttcgggcc tcgacccggt
     3361 gaactccgac gtgatcgacg gcctgatcga gcggctgcaa caggagctcg gcgtgaccag
     3421 cgtggtggta tcgcacgacg tgcgcggctc cttcaaggtg gccgaccgga tcgcgctgct
     3481 gtggcaggcc aagatccgcg ccgccggcac ggccgaggag attctggcct cgcaggaccc
     3541 ggcggtgcag caattcctcg agcgcgatct cgagatgacc ctgctccagg gcggaaagag
     3601 ctgaatggac ctcacctaca agcaggaagt cggtgtcggg gccctcgtgc tggccggcct
     3661 caccctcttc gtggtcgggc tgttctggtt cagcggccgc tcgatccgcc acaaaggcgt
     3721 gtacgcccat gtggtgttca ccaacgtggc gggcctcaag gaaggcgacc cggtgctcgt
     3781 ttcgggcgtc aaggtcgggc gcgtgtccaa ggtcagtctc gagcgggtcg ggcgggtcac
     3841 cgtcacgctc gagctgtcgg gggaggagcg tccgcggacc gatgcatccg ccaccatctc
     3901 ctcgctcgat ttcttcggtg ccaaattcgt ggactacatc cccggctcca aggacgattt
     3961 cctccccgcc accagcgtga tccgcgggac caaatcccag gagctgaccg acattgcgtc
     4021 gggggtggcc acccgggcca acgagctgct cggcaacgcc accggcctcg tctccgacca
     4081 gctggccgac gatatccaca acaccctggt ggccacgcaa cgcggcatgg tggcgctgac
     4141 cgaggtcacg aaggggccgc tcgtcaccca aagcacgaac tcgctcgccg ccctggagcg
     4201 cgtcatggcg cggctcgaca cgctgctcgg ctcggccaac gtcaagggtg cggggcttcg
     4261 cttcgacacg ctgtccgcca acctgcagca cttgtccggc gagctctccc aatccaccag
     4321 ggcactcaac gatctgctgg ggaaggtcaa ccgaggcgaa ggaacgctgg gcaagctcgc
     4381 gagcgacacg ttgctctacg gcaacctcaa caagaccctg gacgcgctga cgaagctgat
     4441 gaacgatctg agagaacggc cgggacggta cctgacggtg aaggtgttct aacggggagt
     4501 ggggagtggt cctcatcggt gagaaccgac gccacggcgc gcaacgacga gaaccactcc
     4561 ccgctcccca ctcccctgtc tctatatttg ccccccatgc tctccgtgac gtttctcggc
     4621 acgtccgccg cgcgcccgac catcgagcgc aacgtttctt ccctggcgct gatgcgcgaa
     4681 ggggagacgc tgctgttcga ttgcggcgag gggacccagc gccagatgat gcgctacggg
     4741 gtgagctttg cgctggacga catcttcttc acccatttcc acggcgatca tttcctgggc
     4801 gtgatcggac tgatccgcac gctcgggctg caggggcgag aggagccgtt gcggttgtgg
     4861 ggcccccggg gagcgaagca ggtgctcaag agcgccatga tgctcggcgt ggaacggctg
     4921 ccgttcgacg tgcagatcga ggaggtcaag gcaggggacg cgatcgggga tgggggacga
     4981 gggacgaggg acgggtacac acttcgcgta ttcgctacgg agcacggcgg cggatcggtg
     5041 ggctacgcga tggtggagaa cgagcgctac gggcgcttcg atcccgacaa agcgcgcgcc
     5101 gccgggatcc cggaaggccc gttatggggc aaactgcagc gtggtgagag cgtggtgttg
     5161 gctgatggcc gacagctgac tgctgatagc gtggtggggc cgcggcggcc ggggaggaaa
     5221 gtcgtgatca ccggcgacac ccggccctgt gcgtcggtgg tcgatgccgc gacgggagcg
     5281 gatctgctgg tacacgaggc cacgttcggg gaggaggaga aggagcggtc ccgggagacc
     5341 atgcactcca ccgcgcgcga ggcggcgcag gtcgctctgg cggcgcaggt ccgcaagctg
     5401 gtgctgagcc acctctcggc gcgctactcg atcaccgccg aggatctggt caaggaggcg
     5461 cgggaggtat tcgtgaacgt cgtcgccgcc aaggacggga tgaccgtgga tgtgcccttc
     5521 gcggagtgat gcagcatgag ggacctcgtt cagcgcgcca ttaccgtacg caggttctgc
     5581 accatcgtgc tcaatcgcgc cggcacattg cggctggggc ggagcttcac cttgagccat
     5641 gcgagcagcg acgggggatt ccagacctgg gtgacggtga gcttggtgga agagccggcg
     5701 aactccaggc ggaagaaggt gcgccagtgg cggcgaccga cgcgctcggc ccagccgaac
     5761 acctggggcg ggttcatgtc caccacttcg atctcggtgt cgcgagcgtc gtagacaatt
     5821 cggatgcggc tgcctttctg gagcgccccc tccggggctt cgacggcgcg ggccgagggt
     5881 agccagagcg ccatgcggtg cgggtcggtg atcgccgcga acacctgttc cgggggcgcg
     5941 ggaacggtgg tggtgagggt gaaggtgacg ggtcgcatga tgcccggaaa tttgcaccag
     6001 aaaccggacg ttggcgaccg gccgccgccg gaactcgcgt cgccggcgtg ttgttaggcc
     6061 tgagtggcca ccggagagtg gctctccacc agccgatgca cgccaaggac gtgagatccg
     6121 tgacacaacc cgaagctgat gtactgagcg ccgagcgcag ctggttcgac ggcctgctgg
     6181 cggcccgcgt gccgcagctc gaggcgctgt tgacgagcga tttccagatc atcgacgtgt
     6241 cgagcggggc ggaggcggat cgcgccgcgg tactgggcgc catcgatgcg ggccagctac
     6301 gcttcgaagc gatcgacgtc ctcgagtcgc gtacgcgtcg ctacggcgat acggtcctcg
     6361 tgaccgggcg cacccggatg cggggccagt ttcaggggcg gccgttcggg gcgcacagcc
     6421 ggtataccca tgtgttcgtg cggcaggaag ggcgctggcg actggcgtcg gcgcagggca
     6481 cgccgatagt cactactccc taggcccggg ggaacccgag agcgcgagag ccgggagcac
     6541 agagcagttc gtccaggtgc gatggaagtc cggctcgcac cgtcacgtac tgctccgtgc
     6601 tccctgctct ttgtcccttt agggttgccg tcccggagcg gggtatctaa gttcagcgtc
     6661 cgcatggggc tgtagctcag ctgggagagc gcctggatcg cacccaggag gtcaggggtt
     6721 cgagccccct cagctccata cataggaact gccccttggt gtaactggca acacgcctga
     6781 ctctggatca ggagagtcct ggttcgagcc caggaggggc aactcagaac gcacggtgat
     6841 gcttgggctt agcggcctga gcgtggccgt gcgctctgtg tttcgagggc ggtgtgtcag
     6901 ttactgtgtc agttacgacg tcagtaacga ggacactccg ggcgacgccc ggcggcgctg
     6961 cggctgctcc acgacctcgc gcatcgtgtc cagatcggcc tggacataac acgtgagcag
     7021 cgtctgcaca tccttccacc ccccggcctc ggcgagatcg cgcaccggat gatgcttgcg
     7081 ctcggtcgcc catttgcgcc ggaacggatg ccaaagtccc ccgggcggcc gacttaggcc
     7141 cgcgtagcga taggcccgct tcagccaatc ggcggccgca tgccggctca gcggcactcc
     7201 cggatcccgt ctcgagggaa agagcaacgc gcccccgatc gcgggatggg ctgcccgctc
     7261 ggccgcgagc gctcgtgctg cccgggtggg catcggggcg tcctgcgtac ggcgtttctt
     7321 gtccagtacg ccccgccaac gaatcacccc tcgggcgaaa tccaagtcgt cccaacgcaa
     7381 tccgagcaca ctcgaaagcc gacggcccgt cgtctccagc agtaagatca ggagagtgag
     7441 ccgcggatgg acccgcgggg ccacggccac gagcgccgtg atcgtcgcat cggcgatcag
     7501 cggccgccgg gtatcctgct cgcgcggcgg atgccaaccg gcgagccggt tgcgctggag
     7561 gtagggtgcc ccgcgatgc
//
LOCUS       DBNGZW010000092         2432 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1777909, whole genome shotgun sequence.
ACCESSION   DBNGZW010000092 DBNGZW010000000
VERSION     DBNGZW010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1777909"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="AC16JC_02310"
     CDS             complement(<1..378)
                     /locus_tag="AC16JC_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HIR5163560.1"
                     /translation="MPRKKRTSETFIRLKQAFHPESLMMTYGYNPEWSEGALKVPIFQ
                     TSTFVFKTAEEGKAFFEIAYGLRAKTEGEPAGLIYSRINNPDLEILEERLALWDGAES
                     CAVFNTGMAAIATTLMAYVRPGDV"
     gene            425..1675
                     /locus_tag="AC16JC_02315"
     CDS             425..1675
                     /locus_tag="AC16JC_02315"
                     /inference="COORDINATES: protein motif:HMM:NF023128.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5163561.1"
                     /translation="MSAPLAPLDRRRVLAWCFYDFANSAYSAVIIATVFSVYFARQIV
                     GNDTGHGDQVWGWAMSTSMAVVALTSPILGAVADRAGVRKRLLLVFTALCVLCVSLFG
                     TIRPGMVWWGFALAVLANIGFEGALVYYNAYLPDIAPAEHRGYVSGLGYGIGYAGSAL
                     GLVIALPLVKTGHYAATWLTVALFLVVFSIPLFRAMPRDVPSGIAARQAAIEGIVHFR
                     RILRDALGMPRLRRFLLALFFYIDGVETTIFFASIYAATTLHFTAGELILMFLAVQLA
                     ALAGAVLLARPIDRWGPKRVIDLSLLVWVGVAAATALTHGKAAFLAIAVIAGTQLGTV
                     QAASRALMASLIPRGKEAEMFGFYAVVGKSSAILGPIVFGTVSHALGGNQRAAVLAVG
                     AFFVLGLVLLQRVSRAATGGSPPA"
     gene            complement(1650..2078)
                     /locus_tag="AC16JC_02320"
     CDS             complement(1650..2078)
                     /locus_tag="AC16JC_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family peroxiredoxin"
                     /protein_id="HIR5163562.1"
                     /translation="MTISKASAVWEGKPKDGRGTYRAASGAFEAPYSFRTRFEGTPGS
                     TPEELIAAAHAACYSMALSADLEKAGTPATRIETEAACTIEAVNGVPTISTMALRVRG
                     TVAGVNQAAFLKAAEGTRDNCPVSRALKGNVRFTLEATLQ"
     gene            2197..>2432
                     /locus_tag="AC16JC_02325"
     CDS             2197..>2432
                     /locus_tag="AC16JC_02325"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5163563.1"
                     /translation="MRKKPETFRARELTPSLTVNDLKQSLAWYEGVLGFHVEDRWEAD
                     GALRGVALRAGTAKLMLTQDDWARGRERHKGEGVR"
BASE COUNT          324 a          841 c          819 g          448 t
ORIGIN      
        1 gacgtcgccg ggacgcacgt aggccatcaa tgtggtggcg atcgccgcca tccccgtgtt
       61 gaacacggcg caggattccg cgccgtccca caacgccagc cgttcctcca gaatctccag
      121 gtcgggattg ttgatgcggc tgtagatcaa cccggccggc tcgccctcgg tcttcgcccg
      181 gaggccgtag gcgatctcga agaacgcctt tccttcttcg gcggtcttga agacgaacgt
      241 ggacgtctgg aagatcggga ccttgagtgc gccctccgac cactccgggt tgtagccgta
      301 ggtcatcatc aggctttcgg gatgaaacgc ctgcttcagg cggatgaacg tctccgaagt
      361 gcgctttttc cgtggcatgg cgtgaagcta gcgggcggca ccggggcgcg ccattttgcg
      421 cgccgtgtcc gctcccctcg ctccgttgga ccgccgccgc gtgctcgcct ggtgcttcta
      481 cgacttcgcg aactcggcgt actcggccgt catcatcgcc acggtcttca gcgtctactt
      541 cgcccgccag atcgtgggga acgacaccgg gcacggcgat caggtctggg gctgggcgat
      601 gtccacctcg atggccgtgg tcgcgctcac ctctccgatc ctcggcgcgg tcgccgaccg
      661 ggccggcgtg cgcaagcgcc tgctgctggt cttcaccgcg ttgtgcgtgc tgtgcgtgtc
      721 gctgttcggc acgatccggc ccggcatggt ctggtggggc ttcgccctcg cggtgctggc
      781 caacatcggt ttcgagggtg cgctggtcta ctacaacgcc tatctccccg acatcgcccc
      841 ggcggagcac cgcggctacg tgtccgggtt ggggtacggc atcggctacg ccgggtcggc
      901 gctcgggctg gtcatcgccc tgccgctcgt caagacggga cactatgcgg cgacctggct
      961 gaccgtcgcc ctgttcctcg tcgtcttctc gatcccgctg tttcgcgcca tgccgcggga
     1021 tgtgccgagc ggcatcgcgg cccgacaggc ggcgatcgag ggaatcgtgc acttccgccg
     1081 catcctgcgg gatgccctcg ggatgccgcg gctgcggcgc ttcctgctcg cgctgttctt
     1141 ctacatcgac ggcgtcgaga cgaccatctt cttcgcgagc atctacgccg cgaccacgct
     1201 gcacttcacc gcgggcgagc tgattctgat gttcctggcc gtccagctcg ccgcgctggc
     1261 gggcgcggtc ctgctcgccc ggccgatcga ccgctggggg ccgaagcggg tgatcgacct
     1321 ctcgctgctc gtctgggtgg gggtggccgc cgcgacggcg ctcacccacg ggaaggcggc
     1381 gttcctggcc atcgcggtga tcgccggaac ccagctgggc accgtgcagg cggccagtcg
     1441 cgcactcatg gcgtcgctca ttcccagggg gaaggaggcc gagatgttcg gcttctacgc
     1501 cgtcgtcggc aagtcgtcgg cgatcctggg gccgatcgtc ttcggcaccg tgtcccacgc
     1561 gctgggcggc aatcagcgcg cggccgtgct cgccgtcggg gcgttcttcg tcctggggct
     1621 ggtgctgctg cagcgcgttt cccgcgcggc tactggaggg tcgcctccag cgtaaagcgg
     1681 acattcccct tcagggcccg cgacacggga cagttgtcgc gcgtcccctc cgcggccttg
     1741 agaaacgccg cctggttcac gccggcgacc gtgccgcgca cccgcagcgc catggtgctg
     1801 atcgtcggca ccccgtttac cgcctcgatg gtgcaggccg cctccgtctc gatgcgcgtg
     1861 gcgggggtcc ccgccttctc gaggtcggcg gacagagcca tcgagtaaca ggcggcatgc
     1921 gccgccgcga tcagctcctc gggcgtgctg cccggcgtcc cttcgaaacg ggtccggaag
     1981 gagtagggag cctcgaaggc gccgctcgct gcccggtacg tcccccgacc atccttcggt
     2041 ttcccctccc acactgcgct ggctttgctg atggtcatcc gcgtctccgg caagagtgtc
     2101 gccgcaatct ggcgagggac ggccccacac gctacgcttc aacaccattc gtcgtcggga
     2161 ggtgccatgg ctgctcctcg caaaccaaag cgccccgtgc gcaagaagcc tgagacgttt
     2221 cgtgcccgtg agctgacgcc gtcattgacc gtgaacgacc tcaaacagag ccttgcctgg
     2281 tacgaaggcg tgctcgggtt ccacgtagag gaccgatggg aagccgacgg cgcgctgcgg
     2341 ggggtcgcgc tgcgagccgg aaccgccaag ctgatgctga cgcaggacga ctgggcgaga
     2401 ggccgcgagc ggcacaaggg cgagggggtc cg
//
LOCUS       DBNGZW010000093         9574 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1849861, whole genome shotgun sequence.
ACCESSION   DBNGZW010000093 DBNGZW010000000
VERSION     DBNGZW010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 9574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9574
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1849861"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..343)
                     /locus_tag="AC16JC_02330"
     CDS             complement(77..343)
                     /locus_tag="AC16JC_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163564.1"
                     /translation="MSRAFINEDAGGPEPRYHLPARDDPGYDAAAAWALIEGANAGDS
                     YSAELATGYRWGEPALRPHVERILDRARAERNERLEQLAERFLR"
     gene            complement(359..628)
                     /locus_tag="AC16JC_02335"
     CDS             complement(359..628)
                     /locus_tag="AC16JC_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163565.1"
                     /translation="MPFHRCAIHKEDGSTLAASVDVMIEELERPGGNEWHGTISTTHL
                     VTLEAGHRYKLVLDDGRSGEFMVRRNTFAGGTNRAVAIQGMGPLR"
     gene            777..1277
                     /locus_tag="AC16JC_02340"
     CDS             777..1277
                     /locus_tag="AC16JC_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR5163566.1"
                     /translation="MTIDPAQAYRLLGLAPGASLEDVKTAYRDLAQVWHPDRFTDNAR
                     LREKAEVNLRRINEAYTVLRDYRAGVAAAAAVVAAAPAAHAAPHVATAGRQAPPLHFD
                     LRHSMFTGVVAMRHSIKVLRPSATYARRPRRRGLPKPWIRLGIAALLFVALAVAALWY
                     KGYLDF"
     gene            complement(1297..2442)
                     /locus_tag="AC16JC_02345"
     CDS             complement(1297..2442)
                     /locus_tag="AC16JC_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163567.1"
                     /translation="MVTSQEPRVVGSVVLYRLYDVGYEIDLERALDFLAATPEEREAL
                     RLKLERRQRAEAQAIRIKNPPVTVTLGSEQFEAAGRRYDASVSARIFDFGVVSLRMRC
                     GIPGALPWSAFAAFGDAAELSAAARPAFDRRIRELTERIAPAVARLQIAPVSEDYVVY
                     RITRLESPAGEALLASAVRELDVAPLLLNEDRALSDQARKELLPHWFSYYPDDLSIVT
                     WNTALVLDPAEGESDEQLILEFANAQLLELRYYDALLDAELPQMYDRIASARKGAGGL
                     LGRRYAPLLTALQTLVADSTEIVERTQNALKVTDDIFLARVYAAALEVFRERAWRTGI
                     DHKLDIIRQTYGMLNAESMAARNELLEIAVVVLILVEIVIAVVLGGH"
     gene            complement(2513..2638)
                     /locus_tag="AC16JC_02350"
     CDS             complement(2513..2638)
                     /locus_tag="AC16JC_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163568.1"
                     /translation="MDKTYRGVCLMVESHVHEHLGQMMAFARTSGVGPPCSRSGQ"
     gene            complement(2723..3826)
                     /locus_tag="AC16JC_02355"
     CDS             complement(2723..3826)
                     /locus_tag="AC16JC_02355"
                     /inference="COORDINATES: protein motif:HMM:NF046871.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecDF P1 head subdomain-containing protein"
                     /protein_id="HIR5163569.1"
                     /translation="MTGLLITGGLSVLPFQTALATRQTLEAPSRIHLVFEPRPVDGSA
                     VQSATLAAIVQVLERRLDDSGVRDASARDSAGRVIVEASGVRELGRLEGLLTRSGRLE
                     FRITDMHHRFRDVLPEIDRTMERPAAPEPPGAPPSADGISQLFGQGASGTTSGPLSAV
                     LFVGQLPGEFLVPEEQVSFAESLLALPQVVVPDGIELWWGVALASGGGHAYRALYALE
                     ARPILTCRDIRDATARRDLTSRHSVVVFHLTGHGATRFCDETGRHVHDYLAIGLDGRV
                     LSTPPIINDRICGEAGQIDLQNAPLADARDLASLMTSGPLPVPLALVDELVLDASSAD
                     RSRLALRTALAAFGVIGLALLLLYAGRRQAELK"
     gene            complement(4052..5785)
                     /locus_tag="AC16JC_02360"
     CDS             complement(4052..5785)
                     /locus_tag="AC16JC_02360"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013764415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5163570.1"
                     /translation="MRRSLLLWTLLLAAPRLDAQRAAVPGAGPRFLVRYPAARSAAPL
                     DGRLLLMLSTDSTAEPRMQISDISETQLIFGRDVDAWAGGRVAVVDGRAEGFPVARLG
                     EVPAGRYRVQALLNRYETYRRADGHVVQLPPDRGEGQQWNRKPGNLYSTPHWITFDPR
                     RSDAFPIDLDQEIPAIPDAPDTKYVRHIRMQSKLLSDFWGRPMFLGAHVLLPFGFDEH
                     PEARYPLVIDHGHFPETFEGWRETPPDPDLKPDSSERFRLAGYNRIQQQAAWQFYHDW
                     TGPGFPRVLLVQIQHATPYYDDSYAVNSANNGPYGDAIMRELIPEIERRFRGIGQGWA
                     RITYGGSTGGWEAMAVQLFYPDEFNGAFIACPEPIDFRQYTVVNIYADTNAYWLPSRW
                     KRVPRPGHRDWLGTVSVTLGQMNQLERVLASHGRSGGQWDIWQAVYGPAGADGYPRPI
                     WDKGTGTIDRAVAAYWREHYDLGALLRRDWATLGPKLRGKLHLYVGEADNYYLNDAVY
                     LVEDFLKRADPPADAEVDYEPRAEHCWNGDHTRPNALSRLRYPQMFIPRLIEQVRRNH
                     PAGADTLSWRY"
     gene            complement(5785..7119)
                     /locus_tag="AC16JC_02365"
     CDS             complement(5785..7119)
                     /locus_tag="AC16JC_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR5163571.1"
                     /translation="MSRKRISRRRFVGDVAAGAAFTIVPRHVLGRGFTAPSDKLNIAC
                     IGCGGKGRSDIDGVAAENIYALCDVDWKQALDAFQHYPRAKRYRDYREMLDRDGKSID
                     AVTVSIPDHSHAAAGLLAMKLGKHAFIQKPLARTLGEVRALEQAARSSKVATQMGNQG
                     HAHDDTRLIREWVEAGAIGTVREVHFWTNRPWWPQGIERPLEEFYVPDSLDWNLWLGP
                     APERPYNPAYAPFKWRGWWDFGTGSLGDMACHIMDAAFWTLDLGYPVRVVPESTPRFA
                     ETAPATSRITYDFPAKGSRPELQVVWQDGALYPPRPPEVAEDAAWPPDDGGGQLWLGS
                     SGKLVAGTYADAPTLLDAAKMAEITAHPVPQKYPRSKGVYEEWIAACKGGPRAGSAFD
                     TYSGPFTEMVLLGCLAVRLGRTLDVDPATGAVKNVTVPPELIQPVYRQGWHL"
     gene            complement(7174..8394)
                     /locus_tag="AC16JC_02370"
     CDS             complement(7174..8394)
                     /locus_tag="AC16JC_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017909363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5163572.1"
                     /translation="MNTRVKLSAMMFLQFFIWGAWYTTIAVYMTQHGMEKLTYWPYTV
                     NPIAAIAAPFFLGLVADRYFATEKVLGTLHLLGGAVMYAVPRFTGAPTLFILLLLVYN
                     LCYMPTLGLANSLAFHHIQAQEKQFPLIRVFGTIGWIIAGLFISYVLGPLTGVVAEGT
                     AWPVYTAATASVLLGLYSFTLPHTPPPAAGQRVSLRSILGLDALSQLGDRSFYVFIVA
                     SFLLCIPLAAYYNFTQLFLGNAGITKIAAVQTLGQVSETGFMLLMPLMFLRLGVKRML
                     MVGMAAWVLRYGLFSIAAPNAIIGLIVIGILLHGVCYDFFFVTGQIYVDKKSTPAVRG
                     QAQGFLVLVTYGAGMLIGAQVAGKVFNRFLGGATSLTLDQWKSFWLLPAGFAALVLVF
                     FAVMFRPKDVTAKT"
     gene            complement(8471..9097)
                     /locus_tag="AC16JC_02375"
     CDS             complement(8471..9097)
                     /locus_tag="AC16JC_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-disaccharide hydrolase"
                     /protein_id="HIR5163573.1"
                     /translation="MNILTAAEQAAGWRLLFDGRTTDGWRGYRRTTMPAGWQVVDGAL
                     TRTGEGGDILTTQKYANFELSLEWKVAPGGNSGVFYRASEDDEAVYWTAPEMQVLDDA
                     RHPDGQSRLTAAGADYGLYEAPAGVVKPAGEWNEARIIVNGRHVEHWLNGVRLLQYEL
                     WSPDWEVRVHNSKFAPHPRYGRNTEGYIGLQDHGDWVAYRNIKIRVLP"
     gene            9370..>9574
                     /locus_tag="AC16JC_02380"
     CDS             9370..>9574
                     /locus_tag="AC16JC_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005002176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HIR5163574.1"
                     /translation="MTYRILADLVALTHFAFVLFVIAGAQLLRHSRWWAVLHVPAFAW
                     GALIEFRGWVCPLTPLENALRARA"
BASE COUNT         1548 a         3336 c         3203 g         1487 t
ORIGIN      
        1 gcgcagacgc gcggctatcc gcctccctac tggacgctgc tcgagctggc ccgccaggaa
       61 gccgcccggc gctgactcag cgcaggaacc gctcggccag ctgctcgagc cgctcgttcc
      121 gctccgcgcg cgcccggtcc aggatccgct ccacgtgcgg ccgtagcgcc ggctcacccc
      181 accgataacc cgtcgcgagc tcggcgctgt aggagtcgcc ggcattggcc ccttcgatca
      241 gcgcccaggc ggccgcggca tcgtagcccg ggtcgtcgcg cgcgggcagg tgataccgcg
      301 gttccggacc accggcgtct tcgttgatga atgctcggga catgcggcgg gatgcggcct
      361 agcggagcgg ccccattccc tgaatggcca cggcgcgatt cgtgccgccc gcgaacgtgt
      421 tgcggcggac catgaattcg ccgctgcggc cgtcgtcgag cacgagcttg tagcggtgac
      481 ccgcttccag ggtgacgagg tgcgtggtgg agatcgtccc gtgccactcg ttcccgccgg
      541 gacgttccag ctcctcgatc atgacgtcga ccgacgcggc caatgtactg ccgtcttcct
      601 tgtgaatcgc gcagcggtga aagggcatgt agcaaagatt gcggcatcgg cgataccctg
      661 caacaacccc atcgccgact ggcgtcattg cccggaccgg cccatacttc aatccgaccg
      721 tcgccagcac acgactcccg gcacgagtct tgcgaccgag gccgccaccg gagcacatga
      781 ccatcgatcc cgcccaagcc taccgtctcc tgggactcgc ccccggcgcg tcgctcgaag
      841 atgtgaaaac cgcgtaccgg gacctcgctc aggtgtggca ccccgaccgg tttaccgaca
      901 acgctcgact gcgtgagaag gcggaagtga acctgcggcg catcaacgag gcgtacacgg
      961 tgctgcggga ctaccgggcg ggcgttgcgg cggctgccgc cgtcgtcgcc gccgcgccgg
     1021 cggcacacgc ggcgccgcac gtcgccaccg cgggacgcca ggccccgccg ctccacttcg
     1081 atctgcgcca ttcgatgttc accggcgtcg tcgcgatgcg ccactcgatc aaggtcctgc
     1141 ggccgagcgc cacctacgca cgcaggccgc gccggcgcgg cctccccaag ccgtggatcc
     1201 gactgggcat cgccgcgctg ctgttcgtgg cgctcgccgt cgcggcgctg tggtacaagg
     1261 ggtatttgga cttctgagcc ctcggggtgg gccggctcag tgcccgccca gcaccaccgc
     1321 gatgacgatt tcgaccagga tcagcaccac caccgcaatc tccagcagct cgttgcgcgc
     1381 cgccatcgac tcggcgttga gcatcccgta cgtctggcgg atgatgtcca gcttgtggtc
     1441 gatcccggtg cgccacgccc gctcgcggaa cacctccagc gccgccgcgt acacgcgggc
     1501 caggaagatg tcgtcggtga ccttgagcgc attctgggtc cgctccacga tctcggtcga
     1561 gtcggcgacc agcgtctgca gcgccgtgag cagcggcgca tagcggcggc ccagcaggcc
     1621 gcccgctccc ttccgcgcgc tcgcgatccg gtcgtacatc tgcggcagct ccgcgtcgag
     1681 cagcgcgtcg taatagcgca gctccaacag ctgcgcgttg gcgaactcga ggatcagctg
     1741 ctcgtcgctc tccccctcgg ccggatccag cacgagggcg gtgttccagg tgacgatcga
     1801 caggtcgtcc ggatagtagg agaaccagtg cggcaggagc tctttgcgcg cctggtcgga
     1861 gagcgcccgg tcctcgttca ggagcagcgg cgccacgtcg agctcgcgca cggcgctcgc
     1921 caggagcgcc tcgccggcgg ggctctccag gcgggtgatc cggtagacga cgtagtcctc
     1981 gctcacgggc gcgatctgca gccgcgcgac cgccggcgcg atgcgctcgg tgagctcccg
     2041 gatccggcgg tcgaacgcgg gcctggccgc agcggacagc tccgccgcgt cgccgaacgc
     2101 ggcgaacgcg gaccagggaa gcgctccggg gatgccgcac cgcatgcgga gcgagacgac
     2161 gccgaagtcg aagatgcgag ccgagacgga ggcgtcgtag cgacgcccgg cggcctcgaa
     2221 ctgctcgctg ccgagcgtga cggtgaccgg gggattcttg atccggatgg cctgcgcctc
     2281 ggcgcgctgc cgccgctcga gcttcaggcg cagcgcctcc cgctcttcgg gagtcgccgc
     2341 gaggaagtcg agggcgcgct cgagatcgat ctcgtagccc acgtcgtaca accggtacaa
     2401 caccacggaa ccgacgacgc gcggctcctg gctcgtcacc accggacggt caccgttgct
     2461 ccaggtcggt tacccagata ttgctctgcc gatcgtcgag ggtgaggttc cgctactgcc
     2521 cgctccgcga gcagggtggc ccgacgccgc tggtgcgcgc gaaggccatc atctgcccca
     2581 gatgctcgtg cacgtgactc tccaccatca gacagacgcc gcgataggtc ttgtccatcc
     2641 cgaagagagg gagcccggcg cggtcgcatg gccccaaggc ccgtgggcgt accttacccc
     2701 cgcctcgcgc actttgtcca cgtcacttca gctcggcctg cctgcggcct gcatacagga
     2761 gcagcagcgc gaggccgatc acaccgaagg cagcgagggc ggtgcgtagc gcgagacggc
     2821 tccgatcggc cgaggaggcg tcgagcacca gctcgtcgac gagcgccaat ggcacgggga
     2881 gcgggccgct cgtcatgagc gaggccaggt ccctcgcatc ggcgaggggg gcgttctgga
     2941 gatcgatctg cccagcctcg ccgcagatcc ggtcgttgat gatcggcggc gtgctcagca
     3001 cccggccatc gagaccgatc gccaggtaat cgtgcacgtg ccggccggtc tcgtcgcaga
     3061 acctggtagc gccgtggcca gtcagatgaa acacgacgac cgaatgccgg ctcgtcaggt
     3121 cgcggcgtgc tgtggcatcg cggatatccc ggcaggtgag aatcggccgg gcttccaggg
     3181 cgtacagggc gcgataggca tggccgcctc ccgaggcgag tgcgacgccc caccacagct
     3241 cgatgccgtc cgggacgacg acctggggaa gcgccagcag gctctccgcg aaggagacct
     3301 gctcttctgg aacgaggaac tcacccggca actggcccac gaacaacacc gcggaaagcg
     3361 ggccggaggt cgtgccgctc gccccctgcc cgaacaactg cgagatgccg tcggcggatg
     3421 gaggcgcccc cggaggctcc ggcgcagccg gccgttccat ggtgcgatcg atctcaggca
     3481 agacgtcgcg aaaccgatgg tgcatatcgg tgatgcggaa ctcgagcctc ccgctccgcg
     3541 tcagcagccc ctcgagccgg cccaactccc tcacacccga agcctccact atcacgcgtc
     3601 cggcggagtc tcgcgcagac gcgtcccgga ctccggagtc gtccaaccga cgttcgagga
     3661 cctgaacgat cgccgcgagg gttgccgact gcaccgcgct gccgtccacg gggcgcggct
     3721 cgaataccag gtgaatgcga ctcggcgctt ccagagtctg ccgcgtcgca agcgccgtct
     3781 ggaaaggaag gacgctcaag ccgccggtga tcagcaggcc ggtcaattct ctcagatgaa
     3841 tctgccgact cgtgtgcatc cgatgaccat cccctgacga cgaataccac atattcgcac
     3901 ccggacggac aattgccggt caactgtggg tcgccgaaac tgcgccgcag ggacgaacag
     3961 gaggatgctc cggctcgatc tcgaggcggt gacggattgc gagggtatcc gtccaggaag
     4021 gtcgggcggc cgaggggacg ccgcaaaccc gctagtatcg ccagctgagc gtatccgcgc
     4081 ccgccggatg gttgcggcga acctgctcga tgagccgcgg gatgaacatc tgcgggtagc
     4141 gcaaccggct caacgcattg ggccgtgtgt ggtcgccgtt ccagcagtgc tccgccctcg
     4201 gctcgtagtc cacttcggca tccgccgggg gatcggcgcg cttcaggaaa tcctcgacca
     4261 ggtacaccgc gtcgttcagg taatagttgt ccgcctcgcc cacgtagaga tgcagcttgc
     4321 cccggagctt cggccccagc gtcgcccagt cgcggcgcag cagggcgccc aggtcgtagt
     4381 gctcccgcca gtatgccgcg accgcgcgat cgatcgtacc agtccccttg tcccagatcg
     4441 gccggggata gccgtcggcc ccggccgggc cgtacaccgc ctgccagata tcccactgcc
     4501 ccccggaacg cccgtgggag gcgagcacgc gctcgagctg gttcatctgg ccgagcgtga
     4561 cgctcacggt gccgagccaa tcgcgatgcc ccggccgcgg cacccgcttc cagcggctcg
     4621 ggagccagta cgcgttcgta tccgcataga tgttcactac ggtgtactgc cggaagtcga
     4681 tgggttcggg gcacgcgatg aacgcgccgt tgaactcgtc cgggtagaag agctgcaccg
     4741 ccatcgcttc ccagccgccg gtcgagccgc cgtaggtgat ccgcgcccag ccctgcccga
     4801 ttccccggaa gcggcgttcg atctcgggga tgagctcccg catgatcgcg tcgccgtacg
     4861 gcccgttgtt cgcggagttc acggcataag aatcgtcgta gtacggcgtg gcatgctgga
     4921 tctgcaccag cagcacgcgc ggaaagcccg gtccggtcca gtcatgatag aactgccatg
     4981 ctgcctgttg ttgaatccgg ttgtatccgg ccaggcggaa ccgctccgac gaatccggtt
     5041 tcagatcggg gtcgggcggg gtttcgcgcc acccctcgaa ggtctcgggg aagtgcccgt
     5101 gatcgatgac gagcggatac cgcgcttcgg gatgctcgtc gaacccgaac ggcaacagca
     5161 cgtgcgcgcc caggaacatg ggccggcccc agaaatcgct cagcagcttc gactgcatgc
     5221 ggatgtggcg gacgtacttc gtgtccggcg catcgggaat cgcggggatc tcctggtcca
     5281 ggtcgatcgg gaaggcgtcg ctgcgccgcg gatcgaacgt gatccagtgc ggcgtgctgt
     5341 acaggttccc cggcttgcga ttccactgct ggccttcgcc gcggtccggg ggcagctgca
     5401 ccacgtgtcc atcggcgcgg cggtacgtct cgtaccggtt caacagcgcc tgtacccggt
     5461 agcgccccgc cgggacctcg cccagccgcg cgaccggaaa cccctcggcg cgcccgtcca
     5521 cgaccgccac gcgaccgccg gcccacgcat ccacgtcgcg tccgaagatc agctgcgtct
     5581 ccgagatgtc ggagatctgc atccggggtt cggccgtcga atccgtggaa agcatcagta
     5641 acagccggcc gtcgagcggc gcggcgctcc gcgccgccgg ataccgtacg agaaaccgcg
     5701 ggcccgcccc cgggacggcg gcccgctggg cgtcgagccg cggcgccgcg agcagcagcg
     5761 tccacaggag cagcgagcga cgcatcacag atgccagccc tgccggtaga ccggctggat
     5821 gagctcgggg ggcacggtca cgttcttcac ggctcccgtc gcgggatcga cgtccagcgt
     5881 ccgccccagt cggaccgcaa ggcagccgag caggaccatt tcggtgaacg ggccggagta
     5941 ggtgtcgaac gccgagccgg cgcgcgggcc gcccttgcag gcggcgatcc actcctcgta
     6001 cacgcccttg gagcgcgggt acttctgcgg tacgggatgg gccgtgattt ccgccatctt
     6061 cgccgcgtcg aggagcgtcg gggcgtcggc gtaggtcccg gctacgagct tcccgctcga
     6121 gccgagccac agctgcccgc cgccgtcgtc gggcggccag gcggcgtcct ccgccacctc
     6181 gggggggcgc ggcggataga gcgcgccgtc ctgccacacg acctgcagct cgggacgcga
     6241 ccccttggcc gggaagtcgt atgtgatgcg ggacgtcgcc ggcgcggtct ccgcaaagcg
     6301 cggcgtggat tccggcacga cgcgcacggg gtagccgaga tcgagcgtcc agaacgcggc
     6361 gtccatgatg tggcaggcca tgtcgcccag cgagcccgta ccgaagtccc accagccgcg
     6421 ccacttgaac ggcgcatacg ccgggttgta gggccgctcc ggcgcgggcc cgagccacag
     6481 gttccagtcg agcgaatcgg ggacgtagaa ctcctcgagc ggccgctcga tgccctgcgg
     6541 ccaccacggg cggttggtcc agaagtgcac ctcgcgcacc gtgccgatcg cgcccgcctc
     6601 cacccattcc cggatcaggc gcgtgtcgtc gtgggcgtgg ccctggttgc ccatctgggt
     6661 cgccactttc gagctgcggg ctgcctgctc cagcgcgcgc acctcgccca gcgtacgcgc
     6721 cagcggcttc tggatgaagg cgtgcttgcc gagcttcatc gccagcaggc cggcggcggc
     6781 gtgcgagtgg tcggggatcg agacggtgac ggcgtcgatg ctcttgccgt ccctgtcgag
     6841 catctcgcgg taatcgcggt aacgcttcgc cctgggataa tgctgaaacg cgtcgagcgc
     6901 ctgcttccag tccacgtcgc acagcgcgta gatgttttcc gcggcgaccc cgtcgatatc
     6961 gctgcggcct ttgccgccgc agccgatgca ggcgatgttg agcttgtccg agggcgccgt
     7021 gaagccgcgc cccaggacgt gccgcggcac gatggtgaac gcggctcccg ccgcgacgtc
     7081 gcccacgaat cgcctccgcg agatgcgctt cctagacatg ctttccccct ctgtacgccg
     7141 ttctcttgac gaatttctct cgacgcctgc tccctacgtc ttcgccgtca cgtctttcgg
     7201 ccggaacatc accgcgaaaa agaccaggac gagagcggcg aatcccgcgg gcaagagcca
     7261 gaagctcttc cactgatcga gggtaaggct ggtcgcgccg cccaggaacc ggttgaagac
     7321 cttgccggcc acctgcgccc cgatgagcat gccggcaccg taggtcacca ggaccaggaa
     7381 cccctgcgcc tggccccgca cggccggggt cgacttcttg tccacgtaga tctgtccggt
     7441 gacgaagaag aaatcgtagc agacgccgtg gagcagaatg ccgatgacga tgagaccgat
     7501 gatcgcgttc ggtgccgcga tggagaacag gccgtaccgc aacacccagg cggccatgcc
     7561 gaccatcagc atgcgcttga cgcccaggcg gagaaacatg agcggcatca gcagcatgaa
     7621 cccggtttcg gacacctgcc cgagcgtctg gaccgccgcg atcttcgtga tgccggcgtt
     7681 gcccaggaag agctgcgtga agttgtagta cgccgctagc gggatgcaca gcaggaacga
     7741 ggccacgatg aacacgtaga aggagcgatc cccgagctgc gacagggcgt cgaggccgag
     7801 gatgctgcgc agcgacaccc gctgcccagc ggccggcggc ggggtgtgag gcagggtgaa
     7861 gctatagaga ccaaggagga cgctggccgt ggcggcggta tagaccggcc acgccgtgcc
     7921 ctccgcaacg acccccgtca gggggcctaa cacgtagctg atgaacaacc ctgcgatgat
     7981 ccacccgatc gtgccgaaga cccggatcag cggaaactgt ttctcctggg cctggatgtg
     8041 atggaacgcc agactgttcg cgagcccgag ggtcggcatg tagcacaggt tgtagacgag
     8101 caacagcagg atgaacaagg tgggagcacc ggtaaaccgc ggcacggcgt acatcaccgc
     8161 gccgccaagc aggtgcaggg tgcccagcac cttctccgtc gcgaaatagc gatcggcaac
     8221 gagtcccagg aagaacggcg cagcgatggc cgcgatcggg ttcaccgtat agggccagta
     8281 ggtgagtttc tccatcccgt gttgggtcat gtacacggcg atggtcgtgt accaggctcc
     8341 ccatatgaag aactgaagga acatcatcgc cgacagcttc acacgagtgt tcatcgtggc
     8401 gtctccacgt ggaggtccgt gagcgcttgc agctgaggcc tgacagctga tggcggatgg
     8461 ctgacggctc ttacggcagc acccggatct tgatgttgcg gtaggcgacc caatcgccgt
     8521 gatcctgcag cccgatgtat ccctcggtgt tgcgcccgta acggggatgc ggtgcgaact
     8581 tgctgttgtg cacccgcacc tcccagtcgg gactccacag ctcgtactgc agcagccgca
     8641 cgccgttgag ccagtgctcc acgtgtcggc cgttcacgat gatgcgcgcc tcgttccact
     8701 cgcccgccgg cttcacgaca cccgccgggg cctcgtacag cccgtagtcc gcaccggcgg
     8761 ccgtgagccg cgactggccg tcgggatggc gcgcgtcgtc caggacctgc atctcgggcg
     8821 cggtccagta cacggcctcg tcgtcctcgc tcgcccggta gaacaccccg ctgttgcccc
     8881 cgggcgcgac cttccattcg agcgacagct cgaagttcgc gtacttctgg gtggtgagga
     8941 tgtcgccccc ctcgccggtg cgcgtcaggg cgccgtccac cacctgccag ccggccggca
     9001 tcgtggtccg cctgtagccg cgccagccgt cggtggtccg gccgtcgaac agcagccgcc
     9061 agccggccgc ctgctcggcg gcggtgagga tgttcacggt cgtggtcgct cgggggctcg
     9121 gggggggtac gttcggcacg gatcgggccg ccggtccgca ggcccacagc gccaacggca
     9181 gcgcggcgcg gcacagcgct cgacagagca tcgcggcatt cctttcgtga ggaactcgcc
     9241 aaaccggctc ggcagggcca agttacgccc ggactcgata ccccgccacg gccccggcac
     9301 gcaccgccgg gaccggagag acggggcgag cccgttccgc actcgtgacg gtacgacctg
     9361 gtgagatcga tgacctaccg tatcctcgcc gacctggtgg cgctcacgca cttcgcgttc
     9421 gtgctcttcg tcatcgcggg cgcgcagctg ctgcggcaca gccgctggtg ggccgtgctg
     9481 cacgtgccgg cgttcgcgtg gggcgcgctg atcgagttcc gcggctgggt ctgcccgctc
     9541 accccgctgg agaacgcgct gcgcgcccgc gccg
//
LOCUS       DBNGZW010000094         4024 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_74244, whole genome shotgun sequence.
ACCESSION   DBNGZW010000094 DBNGZW010000000
VERSION     DBNGZW010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_74244"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="AC16JC_02385"
     CDS             <1..197
                     /locus_tag="AC16JC_02385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163575.1"
                     /translation="PPMGLGVRMLLATGDRFFVYPTKLREYQARYRGSFLHGGVSPEE
                     VILPVALLTPRRAGRGGGVR"
     gene            198..2012
                     /locus_tag="AC16JC_02390"
     CDS             198..2012
                     /locus_tag="AC16JC_02390"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5163576.1"
                     /translation="MAEGRSARLLRLLRPYLPLFALTIGATIVASVLDGFTFVLLIPF
                     LRTLFHQPAIAADGGSAIQALLRKIAGPFLTTGEPAVALRNVVLVLLAALILKNACEY
                     LSAYWSVVIQEGVVRDLRVRLFRHLQTLPLAFFQRTRAGQTVARLITDADQVKTAVSA
                     ALASLIQNCVMIAVYLAVMLGLSVRLTLVALVFAPILLFVIRPLVRRLRRRSREVAAE
                     RGELTAHVSEMMNSVKLVRAYTAESYEAGRFQALASRFRKRVLRAQRFSSLTSPVSEV
                     FGGMMIVAILWYGTLLALQATRGLGPEELITFLIVALRLNSPIKSIANYPAIMAGAAA
                     AADRVFEVLDLPAEETDRPGETAARFAGQIEYHGVSFSYGEEAAVLRDVNFEVRRGEV
                     VAIVGPSGAGKTTLVDLLPRFYEPSSGVILFDGVPITRDTRSSLRGLMGIVSQETILL
                     NDTVLANVAYGKPDYTLEQVQAAARAANAAEFIEHLPEGYHTVLGERGTRLSGGQRQR
                     IAIARALLRDPPILILDEATSALDTESERLVQEAIDRLMRHRTVFVIAHRLTTVQHAD
                     QILVLAEGRIVERGTHDELLARDGLYRRLYHLQFGPAT"
     gene            2009..2875
                     /locus_tag="AC16JC_02395"
     CDS             2009..2875
                     /locus_tag="AC16JC_02395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR5163577.1"
                     /translation="MMLSGFTIVRNAIKLDFPIVPAIRSVLEVCDEVVVNVGKSEDET
                     RDLVAGVNDPRVRIIDSEWDFTRKNEMLSQETLKAMAACRGGWGIYIQADEVLHEDGA
                     RILRDRTREWDADARVEGLLVRYLHFYGGFETVATNRRWYRREVRCIRLGRDIRPYQG
                     AQGFRVGPEYRKIRARATDAVMFHYGWARPARAIREKLEISKIIYPWSRERSAQEQER
                     GYLEWLPLLKPFTGSHPRDAQEWIRERLVDPDRRLGPRRFRWEHLRFYLSDWIERLTG
                     ERLFEFRNYTIV"
     gene            complement(2889..3833)
                     /locus_tag="AC16JC_02400"
     CDS             complement(2889..3833)
                     /locus_tag="AC16JC_02400"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5163578.1"
                     /translation="MHIAVISHHRLPVKGYGGTQRVVVALVRGLVELGHRVTLIAAPG
                     TKLASIPVVEAHARDLHTDGPRLVPYVPRDADIVHAHFPVQSAPAPFPFLQTLHGNMR
                     PDQRIPLNTVFLSRDHAKRHGGTTFVHNGLVPSDFRFRRRKEAWDLFLGRLHSAKGYH
                     WAIEAAKRTGRRLIIAGGWRPNFSGAIKYVGEVNDGQKTALLARARCLWMPALWDEPF
                     GLTTIEALFSGTPVLGTRRGALPEIITPEVGALGDTLEELLDAAGQIHTRDPDCCRAR
                     AERLFSHMAMAKAYVSIYEAVLSTGKLPAGSPAPPAPA"
BASE COUNT          599 a         1362 c         1408 g          655 t
ORIGIN      
        1 tgccgccgat gggactgggc gtgcgcatgc tgctcgccac cggtgaccgc ttcttcgtgt
       61 accccacgaa gctccgcgag taccaggccc gctatcgcgg ctcgttcctg cacgggggcg
      121 tgagtcccga ggaagtgatc ctgccggtgg cgctgctcac gccgcgccgc gccggccggg
      181 gagggggagt ccgctagatg gccgaggggc gcagcgcacg ccttctccgg ctgctccgcc
      241 cgtacctccc gttgttcgcc ctcaccatcg gcgccaccat cgtcgcgtcg gtgctcgacg
      301 gcttcacgtt cgtcctgctg atcccgttct tgcgcacgct gttccaccaa cccgcgatcg
      361 cggccgacgg cggctccgcg atccaggcgc tgctgcgcaa gatcgcgggt ccgtttctca
      421 cgaccggcga gcccgcggtg gcgctccgca acgtggtcct ggtgctgctc gcggcgctga
      481 ttctcaagaa cgcctgcgag tacctgtccg cctactggtc ggtggtgatc caggaagggg
      541 tggtgcgtga cctgcgggtg cgcctgttcc gccacctcca gaccctgccg ctcgccttct
      601 tccagcgcac cagggccggc cagacggtgg cgcggctcat caccgacgcg gaccaggtca
      661 agaccgcggt cagcgcggcg ctcgcgtcgc tgatccagaa ctgcgtgatg atcgccgtct
      721 acctggccgt gatgctcggg ttgtccgtcc gtctcaccct cgtggcgctg gtcttcgcgc
      781 ccatcctgtt gttcgtcatc cgccccctgg tgcggcgact gcgccgtcgc tcacgcgagg
      841 tggcggccga gcggggcgag ctgacggcgc acgtgagcga gatgatgaac tcggtgaagc
      901 tggtccgggc ctacaccgcc gagtcgtacg aggcgggacg gttccaggcg ctggccagcc
      961 gcttccggaa gcgcgtactc cgcgcccagc gcttctccag cctcacgagc cccgtgagcg
     1021 aggtgttcgg cggcatgatg atcgtcgcca ttctctggta cggaacgctg ctggcgctgc
     1081 aggccacgcg cgggctgggg cccgaggagc tgatcacctt cctcatcgtg gcgctgcggc
     1141 tcaattcgcc gatcaagtcc atcgccaact acccggcgat catggccggc gcggcggcgg
     1201 cggccgaccg ggtgttcgaa gtgctggacc tccccgccga ggaaacggac cgtcccggtg
     1261 aaacggcggc gcgcttcgcg gggcagatcg agtaccacgg cgtgtccttt tcctacgggg
     1321 aggaggcggc cgtgctgcgc gacgtgaatt tcgaggtgcg acgcggcgaa gtcgtggcga
     1381 tcgtcggccc gtccggggcg gggaagacca cgctggtcga cctgctgccc cgcttctacg
     1441 agccgtcgag cggcgtgatc ctgttcgacg gcgtgccgat cacccgggac acgcgctcgt
     1501 cgttgcgcgg cctcatgggg atcgtgtcgc aggagacgat actgctgaac gacacggtgt
     1561 tggccaacgt cgcctacggg aagccggact acacgctgga gcaggtgcag gccgccgcgc
     1621 gggcggcgaa cgccgcggag ttcatcgagc acctccccga gggctatcac acggtgctgg
     1681 gcgagcgcgg cacgcggctg tcgggcgggc agcgccagcg catcgcgatc gcccgcgcgc
     1741 tgctgcgcga cccgccgatt ctcatcctcg acgaagcgac gagcgccctc gacaccgaat
     1801 cggagcgcct ggtgcaggag gcgatcgacc ggctgatgcg gcaccggacg gtgttcgtca
     1861 tcgcgcaccg gctcaccacc gtgcagcacg ccgaccagat cctcgtgctg gccgaaggcc
     1921 gcatcgtgga gcgcggcacc cacgacgagc tgctggcgcg cgacggcctg tatcgccggc
     1981 tctatcacct gcaattcggg cccgccacgt gatgctgtcc ggcttcacca tcgtccgtaa
     2041 cgcgatcaag ctcgatttcc ccattgtccc cgcgatccgc tcggtgctcg aggtgtgcga
     2101 cgaggtcgtg gtgaacgtgg gcaaatccga ggacgagacc cgggatctcg tggccggcgt
     2161 gaacgacccc cgggtgcgca tcatcgacag cgagtgggac ttcacccgca agaacgagat
     2221 gctgtcgcag gagaccttga aggcgatggc cgcctgccgc ggcggttggg ggatctacat
     2281 ccaggcggac gaggtgctgc acgaggacgg ggcgcgcatc ctgcgggacc ggacgcggga
     2341 atgggacgcc gatgcgcggg tggagggtct gctggtgcgc tatctccatt tctacggcgg
     2401 cttcgagacc gtggcgacca accgccgctg gtatcgccgc gaggtgcggt gcattcgcct
     2461 ggggcgcgac atccgcccct atcagggggc gcaggggttc cgggtcggcc ccgagtaccg
     2521 gaagatccgc gcccgcgcca ccgacgccgt gatgtttcac tatgggtggg cccgccccgc
     2581 gcgcgccatc cgcgagaagc tcgagatctc caagatcatc tatccctgga gccgtgagcg
     2641 ttcggcgcag gagcaagagc gcggctacct ggaatggctg cccctcctga aacccttcac
     2701 tggatcccat ccgcgggacg cgcaagagtg gattcgggag cggctggtgg atcccgatcg
     2761 gcgcctcggc ccgcgccggt ttcgctggga gcacctgcgg ttctacctgt cggactggat
     2821 cgaacggctg accggggaac gcctcttcga gttccgcaac tacacgatcg tctagaacgc
     2881 ggggccgatc acgcgggagc ggggggagcc ggggagccgg cggggagctt cccggtggag
     2941 agcacggcct cgtagatcga cacgtaggcc ttcgccatcg ccatatggga gaacaagcgc
     3001 tcggcccggg cacggcaaca gtcgggatcc ctggtgtgga tctgcccggc tgcgtcgagc
     3061 agctcttcca acgtgtcgcc gagcgcgccg acctcgggcg tgatgatctc ggggagggcg
     3121 ccgcgtcgcg tgcctaggac cggcgttccc gagaacagcg cttcgatcgt ggtgaggccg
     3181 aacggctcgt cccacagcgc cggcatccac agacagcggg cccgcgcgag gagggcggtc
     3241 ttctgaccgt cgttgacctc gcccacgtac ttgatcgccc ccgagaaatt ggggcgccac
     3301 ccaccggcga tgatcaagcg ccggccggtg cgcttcgcgg cttcgatggc ccagtgatac
     3361 cccttggcac tgtggaggcg ccccaggaag agatcccacg cctccttacg tcgccggaac
     3421 cggaaatcgg agggcacgag gccgttatgt acgaacgtcg ttccaccgtg gcgtttggcg
     3481 tgatcacgcg agagaaacac ggtattgaga ggaatccgct ggtcgggccg catgttgccg
     3541 tgcagcgttt gcaggaatgg gaacggcgcg ggcgccgatt gcacgggaaa gtgtgcgtgc
     3601 acgatgtcag catcccgcgg cacatagggg acgagcctag ggccgtcggt gtgcaggtcg
     3661 cgcgcatgcg cttcgacgac cgggatcgaa gcgagcttcg tgcccggtgc ggcgatcagc
     3721 gtcacgcgat ggccgagctc gacgagtccg cgcaccagcg cgaccacgac gcgctgcgtg
     3781 ccgccgtacc ccttcaccgg caggcggtgg tgcgagatca cggcgatgtg catcggggcc
     3841 acagtataac tagcttcgct ccatgcgcga ggccccgctc accgtcttgc agctgacctt
     3901 tcagggcgac ggcgccggct cgacgcaatc catcctcaac ctgagcgagc agctcgcgcg
     3961 gcgcggggcg cgcgtcctgg tcggctgccg ggccgagtcc ctgctgttcc ggtcggcgcg
     4021 cgcc
//
LOCUS       DBNGZW010000095         6934 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_415044, whole genome shotgun sequence.
ACCESSION   DBNGZW010000095 DBNGZW010000000
VERSION     DBNGZW010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6934)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6934)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6934
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_415044"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..515
                     /locus_tag="AC16JC_02405"
     CDS             <1..515
                     /locus_tag="AC16JC_02405"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR5163579.1"
                     /translation="AGPAKPLVDDVRREIQASSPDLPYPGIDPMPQLYADQLQPWRVG
                     SSLFALFGGLGVLLAAIGLYGVLSYVVSQRTQELGIRIALGAGRSSVLGLVLRQGLKV
                     TLVAVVLGAAGALAAGRAIASLLYGVSPHDPLVFGAVALLLTLVAVVASYLPAYRATR
                     VDPMVALRTE"
     gene            605..898
                     /locus_tag="AC16JC_02410"
     CDS             605..898
                     /locus_tag="AC16JC_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163580.1"
                     /translation="MVNLLHDVRTTGISLAALLSLGMSGGQTRCPARSARAAGPQPTT
                     PAPAHAPAPAPTLASSWMDYVDFRRGSGDALTNLGGPALAATRARPRHPTPDS"
     gene            924..1586
                     /locus_tag="AC16JC_02415"
     CDS             924..1586
                     /locus_tag="AC16JC_02415"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5163581.1"
                     /translation="MIALHNIEKAYPQGGGKVFVLRQVSLDIRPGEFVTVMGPSGAGK
                     STLLAILGMLDGEWTGEYRLFDHVVHALKPRDRVALHKQYVGFVFQQYHLLDDLTVAE
                     NLDIPLSYRDIKKGERQAIVADTLDRFGMVAKKDLYPRQLSGGQQQLVAVARAVIHKP
                     RILLADEPTGNLHSDQGREIMELFKRLNAAGTTIVQVTHSEVNAAYGNRIVQLKDGWI
                     VN"
     gene            1679..4111
                     /locus_tag="AC16JC_02420"
     CDS             1679..4111
                     /locus_tag="AC16JC_02420"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5163582.1"
                     /translation="MEALLQDIRHAIRSLRTHPGFTVVALLTLALGIGANTAIFSVVN
                     GVLLAPLPYREPERLAVVHHFYPSLQNLRASVSVPGFRDYRARTDIFSSAAVENDMAM
                     NLTGSGQPERVTVILVSGDFFPMFGVTPALGRALRPDEAEAGKNHVVVLSWGFWQRHF
                     GGDRTVAGRRLLLNNESYEVVGVMPASFKDFAQRTADLWTPITFQPADFADNRRTNEF
                     LTFIGRLAPGTSLEHAQAVMHTFATQLRNEYRDNYAPDWDLQVTALADEAARNVRGGL
                     LLLLGAVAFVLLIACANVANLQLARTAAKARDIAVRVALGASPRRLVRHLLTESVVLA
                     LAGGALGLLLAAWGVPALLSLVGTGLPPTAQVGLDLRVLGFALFVSLATGFLFGLAPA
                     LHMARTDLHESLKEGGRAVGQRSSLALRRGLVVTTVALALTLLVGAGLLIRSFARLVD
                     VNPGFQADHLLTFYVSLPDATYPNDTVRVAALQRITQAIEAVPGVVSAGGTSNIPFGG
                     NWSTASFNVEGYQPPANTPTPWGDMRTVTPGYLAAMRAPLIAGRQFTDADRAGAQPVC
                     IVDQEMVRRYWPNIDPIGKRITFNDLGDSSIRWIQVVGVVGHMAHEGLDADKRVQVYF
                     PLPMNPLPFLGFTVRTTGEPASAVGAIRAAVQSVDANLPLASVATMDDLIGQSLGPRR
                     FSLLLLAGFAVLALVLAAIGLYGVMSYTVTQRARELGVRVALGASTRDVVGLVLRQGV
                     RLVILGVGIGLGAALLLTRVMRTMLFDISATDPLTFVAIPLLLIGVALVASYLPARRA
                     TVVDPVVALRTE"
     gene            4195..6609
                     /locus_tag="AC16JC_02425"
     CDS             4195..6609
                     /locus_tag="AC16JC_02425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5163583.1"
                     /translation="METLLQDIRLALRQLARAPGFTVSAVLTLALGLGANTAIFSVVD
                     AVLLRPLTFQDPSRLVLVWERFAARDQATNVVNPANYLDWRDRATSFSDLAALGWSQQ
                     TFTGDVPELVQGRSVTPNFFRTLGLAPLLGRVFTPAEALPGGPPVIVLSYGLWRRRFG
                     GDSSVVGRPVPVAGGSAVVLGVMPPALRPMPWGSEEYWEPFRLNEGDRTRSGRYAMVV
                     GRLKPGVTVARAQAEMDVITRGLQQEYPNFDTGWTANVVALTDQVVGSARRALLVVLG
                     AVTLVLLIAAANVGNLVLVRAEARQRELTVRAALGASRLRLVRQWLVESALVSLAGGA
                     AGVMLARWGVDLLVALAPPYVPRLAEITVDARVLGVAALVSLAVGIGAGLPAALGVGA
                     HDLAAGLRGGSSRTTGDRVARRWRDGLVVAQVSLALVLLSGAGLLVRSLQRLSAIDPG
                     FEPAHVTSMAVALPQAKYGDGAHQTAFYQQLTDRIRTMPGVSEVGAISLVPLVPQNSA
                     TRFTIVGRPVPQPGEWTSADIRIVEPGYFAAMRIPVARGRTFTAADRADAPPVVVVNE
                     AMARQFWPGDDPLGARLQVSWADATAHPEIVGVVGDVRTSALDAEIRPMIYYVEAQAP
                     TGWLTLVVRHRGDVTALVSAVRGAVRDLDRDVPLTDVASLSTLLTRSMSDRRYPMLLL
                     SGFAVLAVLLAAVGLYGLLSYAVSRRNQEIGVRMALGASRGTVLGMVMKNGVRLTLAG
                     VALGAAGGVLAARALGRLLYDIGPTDPVTFVAVAVLLVAVALLASYVPAARATRVDPV
                     VALRTE"
     gene            6693..>6934
                     /locus_tag="AC16JC_02430"
     CDS             6693..>6934
                     /locus_tag="AC16JC_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020717055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163584.1"
                     /translation="MEALLQDIRYGIRRLARSPGFTGLAVLVLALGIGGTATAFGVVN
                     AVLLRPLGFPDPGALVRPYSLWRGSMSTVSAPDFAD"
BASE COUNT          920 a         2441 c         2420 g         1153 t
ORIGIN      
        1 ccgccggccc cgccaagcct ttggtggacg acgtccgccg ggagatccag gccagctccc
       61 cggacctacc gtatcccggc atcgatccca tgccccagct gtacgcggat cagctgcagc
      121 cctggcgggt cggctcgtct ctgttcgcgc tgttcggcgg gttgggcgtg ctgctcgcgg
      181 cgatcggcct gtacggcgtg ctgagctacg tcgtctccca gcgcacgcag gagctgggga
      241 tccgcatcgc cctgggggcc ggccggagca gcgtgctggg actcgtgctg cggcagggcc
      301 tgaaggtcac gctcgtcgcg gtcgtgttgg gcgcggccgg cgccctcgcc gcgggccggg
      361 cgatcgcctc gctgctgtac ggcgtgtcgc cgcacgatcc tctggtgttc ggcgcggtcg
      421 cgctgctgct gacgctcgtc gcggtcgtcg ccagttatct ccccgcgtac cgtgcgacgc
      481 gggtcgatcc gatggtggcg ttgagaacgg aataggtgcc aggtgccggg tgtcagatgt
      541 cagggccttt acctgacacc cggcacctga cacagggctt gtggaaccac tggaggggag
      601 cgatatggtc aaccttctcc acgacgttcg caccacaggg atctcgctgg ctgcgctgct
      661 gtcgctcggc atgagcggtg gacaaacgcg gtgccccgct cgctccgcgc gggccgcggg
      721 cccgcagccc acgacgcccg ccccggccca tgccccggct cccgcaccga ccctggcctc
      781 gtcctggatg gactacgtcg acttccgccg tggctcgggg gacgccctga cgaacctggg
      841 gggccccgcg ctggcggcga cgcgtgccag accccgacac ccgacgccgg actcctgaca
      901 tctgacatct gacaccggaa cccatgatcg cactgcacaa catcgaaaag gcgtatcccc
      961 agggcggcgg caaggtgttc gtgctgcgcc aggtctcgct cgacatccgt ccgggtgagt
     1021 tcgtgaccgt catggggccc tccggcgccg gcaagtccac cctgctcgcc atcctcggca
     1081 tgctcgacgg ggaatggacc ggcgagtacc gcctgttcga tcacgtcgtg cacgcgctca
     1141 agccgaggga tcgcgtcgca ctgcacaagc agtacgtggg cttcgtgttc cagcagtatc
     1201 acctgctcga cgatttgacg gtcgccgaga acctcgacat cccgctgtcg tatcgcgaca
     1261 tcaagaaggg cgagcgccag gcgatcgtcg ccgacacgct ggatcgcttc ggcatggtgg
     1321 cgaagaagga cctctacccg cggcagctct cgggcggcca gcagcagctg gtcgccgtgg
     1381 cacgggcggt gatccacaag ccccggattc tcctcgccga cgagccgacc ggcaacctcc
     1441 actccgacca gggccgcgag atcatggagc tgttcaagcg gctcaacgcc gccggcacga
     1501 cgatcgtgca ggtgacgcac tcggaagtca acgcggcgta cgggaaccgg atcgtgcagc
     1561 tgaaagacgg ctggatcgtg aactgaaagg tgccaggtac caggtatcag gtgtcggcgt
     1621 tgggggggcg cgccagacac ctgacacccg acacctggca cctctgggga ggcagcatat
     1681 ggaagccctg ctgcaagaca tccgccacgc catccgctcg ctgcgcaccc acccgggatt
     1741 caccgtggtc gcgctgctca cgctcgccct cgggattggg gcgaacaccg cgatcttctc
     1801 ggtggtgaac ggcgtgctgc tggcaccgtt gccataccgg gagccggagc ggttggcggt
     1861 cgtgcaccac ttctatccct cgctccagaa cctgcgggcg tccgtctcgg tgcccggctt
     1921 ccgcgactac cgggcgcgga ccgacatctt ttccagcgcc gccgtcgaaa acgacatggc
     1981 gatgaacctc accggatcgg gccagccgga gcgggtgacc gtgatcctgg tgtccggcga
     2041 cttcttcccg atgttcgggg tcacgccggc gttgggccgc gcgctccggc ccgacgaagc
     2101 ggaggcgggc aagaatcacg tcgtcgtgct gtcgtggggg ttctggcagc ggcacttcgg
     2161 cggcgaccgc accgtggccg gccgccggct gttgctcaac aacgaatcct acgaggtcgt
     2221 cggcgtcatg ccggcgagct tcaaggattt tgcccagcgc accgcagatc tgtggacgcc
     2281 gatcacgttc cagccggcgg acttcgccga caaccggcgg accaacgagt tcctcacctt
     2341 catcggtcgc ctggcgccgg gcacctcgct cgagcacgcg caggcggtga tgcacacctt
     2401 cgccacacag ctgcgcaacg agtaccggga caactacgcc cccgactggg acctgcaggt
     2461 caccgcgctg gccgacgagg cagcccgcaa cgtgcgcggc gggttgctgc tgctgctcgg
     2521 ggcggtggcc ttcgtgctcc tgatcgcgtg cgccaacgtc gccaacctgc agctggcccg
     2581 caccgccgcg aaggcgcggg acatcgccgt acgggtggcg ctcggggcga gtccgcgacg
     2641 gctggtgcgc cacctgctca ccgagagcgt cgtgctcgcc ctggcgggcg gggcgctggg
     2701 gctgctgctc gcggcgtggg gcgtgccggc gctgctgtca ctcgtcggca ccgggttgcc
     2761 gcccacggct caggtcggcc tggacctgcg ggtgctgggg ttcgcgctgt tcgtttcgct
     2821 cgccaccggg ttcctcttcg ggctcgctcc ggcgctgcac atggcccgga cggatctaca
     2881 cgagtcgctc aaggaagggg gacgggccgt cggccagcgc tcgagcctgg ccctgcggcg
     2941 cggcctcgtg gtaaccaccg tcgccctggc cctcacgctg ctcgtcggtg cggggctgct
     3001 gatccgcagc ttcgcccggc tggtagacgt gaaccccggc ttccaggccg atcacctgtt
     3061 gaccttctac gtatcgctgc cggatgcgac gtacccgaac gacacggtgc gggtcgcggc
     3121 gttgcagcgg atcacccagg ccatcgaggc cgttcccggc gtcgtgagcg ccgggggaac
     3181 gagcaatatc ccgttcggcg gcaactggtc caccgcgagc ttcaacgtcg agggctatca
     3241 gcctccggcc aacacgccca cgccgtgggg cgacatgcgc acggtcacgc ccgggtatct
     3301 cgccgcgatg cgggcaccac tcatcgccgg ccgccagttc accgacgccg accgcgccgg
     3361 cgcgcagccc gtctgcatcg tcgatcaaga gatggtgcga cgttactggc ccaacatcga
     3421 tcccatcggg aagcggatca ccttcaacga cctcggcgac tcgagcattc gatggatcca
     3481 ggtcgtgggc gtcgtggggc acatggccca cgaagggttg gacgccgaca agcgcgtcca
     3541 ggtctacttc cccctgccca tgaaccctct gcccttcctc ggcttcacgg tgcggacgac
     3601 gggtgaaccg gcgtccgcgg tgggcgcgat ccgcgccgcg gtgcagtcgg tggacgccaa
     3661 cctgcccctg gccagcgtgg cgacgatgga cgacctgatc gggcaatcgc tggggccgcg
     3721 gcgcttcagc ctgctgctgc tcgccgggtt cgcggtcctc gccctggtgc tggccgcgat
     3781 cggcctctat ggcgtcatga gctacacggt gacgcagcgt gcccgcgagc tgggcgtgcg
     3841 cgtcgcattg ggcgcgagca cccgcgacgt cgtgggactc gtgctccggc agggggtgcg
     3901 cctcgtgatc ctcggagtgg gaatcggcct cggggcggcg ctgctgctca cccgcgtgat
     3961 gcgcaccatg ctgttcgaca tcagcgccac cgatcccctc accttcgtgg ccattccgct
     4021 gctgctcatc ggcgtggcgc tggtcgcgag ctacctgccg gcgcgccggg cgacggtggt
     4081 ggacccggtg gtggcgttgc gaactgaata ggtgtcgggt gccaggtgtc gggtgtcagg
     4141 tggtcgtttc tggcacctga cacccggctc ctgacacctg cctctggggg cacaatggaa
     4201 accctgctcc aagacatccg cctcgcgctt cgccagctcg ccagggcccc gggcttcacc
     4261 gtcagcgccg tgctcaccct cgcgctcggg ctcggcgcga acaccgcgat cttctccgtg
     4321 gtcgacgccg tgttgctgcg tcccttgacg ttccaggacc cgagccggct ggtgctcgtg
     4381 tgggagcggt tcgcggcgcg cgaccaggcg accaacgtgg tcaatccggc caattatctc
     4441 gactggcgcg atcgggcgac cagcttctcc gacctggcgg cgctcggctg gtcgcagcag
     4501 acgtttacgg gagacgtccc cgagctcgtg cagggccgtt ccgtcacgcc gaacttcttc
     4561 cgcaccctcg ggctcgcccc gttgctcggc cgtgtcttca cgcccgccga ggcgctgccc
     4621 ggcggtccgc ccgtcatcgt gctgagctac gggctgtggc gccgccggtt cggcggtgac
     4681 tcgagcgtcg tcgggcggcc ggtgccggtg gccggcggca gcgccgtggt cctcggggtg
     4741 atgccgcccg cattgcgtcc catgccgtgg ggaagtgaag aatactggga accgttccgg
     4801 ctcaacgaag gcgaccgcac ccgcagtggg cggtacgcga tggtcgtggg acgactcaag
     4861 cccggcgtca cggtcgctcg cgcgcaggcg gaaatggacg tcatcacccg aggcctgcag
     4921 caggagtacc ccaacttcga taccggctgg accgcgaacg tcgtcgcgct gacggaccag
     4981 gtcgtaggtt cggcacggcg cgccctgctg gtcgtgctcg gcgccgtcac gctggtgctg
     5041 ctcatcgccg ccgccaacgt cgggaacctc gtgctcgtac gggcggaggc gcggcagcgg
     5101 gagctcacgg ttcgggcggc gctgggcgcg tcccgcctgc gcctcgtgcg gcaatggctc
     5161 gtggagagcg ctctggtgtc gctcgcggga ggcgcggccg gcgtgatgct ggcgcgctgg
     5221 ggtgtggatc tgctggtggc gctcgcgccg ccatacgtcc cgcgccttgc ggagatcacg
     5281 gtggatgcac gcgtgctcgg ggtggcggcc ctggtctcgc tggccgtcgg gatcggggcc
     5341 gggttgccgg cagcgctcgg cgtcggcgcc cacgacctgg ccgccggctt gcgcggcggg
     5401 agcagccgca ccacgggcga ccgcgtcgcg cggcgctggc gcgacggcct ggtcgtcgcc
     5461 caggtgtcgc tcgccctggt gctgctcagc ggcgcggggc tcctcgtacg ctcgctgcag
     5521 cggctgtcgg cgatcgaccc cggattcgag cccgcccacg tgacgtccat ggccgtcgcc
     5581 ctgccccagg cgaagtacgg ggacggtgcg catcagacgg ccttctacca gcagctcacc
     5641 gatcgcatcc ggacaatgcc gggggtgtcc gaagtcggcg ccatcagcct cgttcccctc
     5701 gtcccccaaa actctgcgac gcgcttcacc atcgtggggc gccctgtccc gcagccgggt
     5761 gagtggacca gtgccgacat ccgcatcgtg gagccgggct acttcgcggc catgcggatc
     5821 ccagtggcac gcggccggac cttcaccgcg gcggaccgcg ccgacgcccc cccggtcgtg
     5881 gtcgtgaacg aggcgatggc gcggcaattc tggccggggg acgatcccct cggcgcacgg
     5941 ctgcaggtga gctgggccga cgcgacggct catccggaaa tcgtgggcgt ggtcggcgac
     6001 gtgcgcacca gcgccctcga tgcggaaatc cgccccatga tctactacgt cgaggcgcag
     6061 gcgccgaccg gatggctgac gctggtggtg cgccaccgcg gggacgtcac cgcgctcgtt
     6121 tccgccgtgc gaggcgccgt ccgcgatctc gaccgcgacg tgccgctcac ggacgtcgcg
     6181 tcgctgtcca ccctgctcac gcggtcgatg tcggatcgcc gctacccgat gttgctcctg
     6241 tccgggttcg cggtgctggc ggtgctgctc gccgccgtcg gcctgtacgg tctgttgtcg
     6301 tacgccgtct cgcgccgcaa tcaggagatc ggcgtgcgca tggcgctcgg agcaagccgt
     6361 ggcaccgtcc tcggcatggt aatgaagaac ggcgtacgcc tgacgctcgc aggcgtggcg
     6421 ctcggcgccg cgggcggcgt gctggccgca cgagcgctgg gccgtctcct gtacgacatc
     6481 ggccccacgg atcccgtcac cttcgtggcg gtggccgtcc tgctcgtggc cgtggcgctg
     6541 ctggccagtt acgtcccggc cgcgcgggcg acgcgggtgg atccggtggt ggcgctgaga
     6601 actgaatagg tgtcgggtgg caggtgtcgg gtgtcagggg atcgtttctg gcacctgaca
     6661 cccggcacct gacacctgct tctggaggca caatggaagc cctgctgcaa gacatccgct
     6721 acggcatccg ccggctcgcc cggagtcccg gattcaccgg gctcgcagtg ctggtgctgg
     6781 cgctcggcat cggcggcacc gcgacggcct tcggcgtcgt gaacgccgtg ctgctgcggc
     6841 cgctcggctt tcccgatccc ggcgctctgg tgcgccccta cagcctgtgg cgcggcagta
     6901 tgtccaccgt ctccgcaccc gatttcgcgg actg
//
LOCUS       DBNGZW010000096         1639 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_486828, whole genome shotgun sequence.
ACCESSION   DBNGZW010000096 DBNGZW010000000
VERSION     DBNGZW010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1639)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1639)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1639
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_486828"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..882
                     /locus_tag="AC16JC_02435"
     CDS             <1..882
                     /locus_tag="AC16JC_02435"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5163585.1"
                     /translation="LPPFLFAGLRFVAAGALLGAGALAFGDHLPRRAVDWQRAAIAGV
                     LLLAGGNAFVVWAEQYTPSGVTSVFVVTVALWMAFFDSVIPGGSGSLGWRVVLGLFTG
                     FLGTALLVGANPREILAADLRGPIALTSASVFWSLGSIYAKRHRTEASPYMTAAIQML
                     AGGIVVTLVGTGLGEWTQWHMSGRGALAFGYLVVFGSIVGYSAYGYALRHASATMVGT
                     YAYVNPVIAVLLGWLLLDEHVTGRMLVAIGLILAAVVWIQFSHKTSTVVGPRSSGVTP
                     TTDDRRPRTSVRHTEAA"
     gene            879..>1639
                     /locus_tag="AC16JC_02440"
     CDS             879..>1639
                     /locus_tag="AC16JC_02440"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR5163586.1"
                     /translation="MTETTLDPQNWDELRTLGHRIMDDMFDYLRGVRERPVWQPFPEE
                     VKTRLAEPVPHRGRPADAVYEQFVRDVLPYPPGNIHPRFWGWVIGTGTPLAMLADLLA
                     SGMNPQSAGIQEGARYIEERVIAWLAELMGFPQGSSGLLVSGGSMANLVGLAVARQAK
                     AGFDVREAGMGDGAPALVVYASTEVHSSVRKAVELMGLGRRNLRLVPVDGEYRIDVQQ
                     LRAAIARDRAEGRRPICVVGNAGTVNTGATDDLRAL"
BASE COUNT          215 a          554 c          591 g          279 t
ORIGIN      
        1 ttgcccccgt tcctgttcgc gggactgcgg ttcgtcgccg ccggtgcact gctcggcgca
       61 ggcgcgctgg cgttcggcga tcacctcccg cgacgcgccg tggattggca gcgtgcggcc
      121 atcgcgggcg tcctgctgct cgcgggcgga aacgccttcg tggtctgggc cgagcagtat
      181 acgccctcgg gcgtgacgag cgtcttcgtg gtgaccgtcg cgctctggat ggcgttcttc
      241 gactcggtga tccccggcgg ctccgggtcc ctgggctggc gcgtcgtgct cggcttgttc
      301 accggcttcc tgggtaccgc cctgctcgtc ggcgccaatc cgcgcgaaat cctcgcggcg
      361 gatttgcgcg gcccgatcgc gttgacgagc gccagcgtct tctggtcgct cggctcgatc
      421 tacgccaagc ggcatcgtac ggaggcgagc ccgtacatga ccgcggcgat ccagatgctg
      481 gcgggcggta tcgtcgtgac gctcgtgggg acagggctgg gcgagtggac ccagtggcac
      541 atgagcgggc gcggggccct cgccttcggc tacctcgtcg tgttcggctc gatcgtggga
      601 tacagcgcct acggctacgc cctgcggcac gcctccgcga ccatggtggg gacctacgcg
      661 tacgtcaatc cggtgatcgc cgtgctgctg ggctggctgt tgctggacga gcacgtgacg
      721 gggcgcatgc tggtggcgat cggcttgatt ctcgccgcgg tggtctggat tcaattctct
      781 cataagacgt ccacggtagt cggtccgcgg tcgtcgggag tcacaccgac gaccgatgac
      841 cggcgaccga ggacatcggt acgccatacg gaggccgcgt gacggaaacg accctcgatc
      901 cccagaactg ggacgagctg cgcaccctcg gccatcgcat aatggacgac atgttcgact
      961 acctgcgcgg ggtgcgcgag cgccccgtct ggcagccgtt cccggaggag gtgaagaccc
     1021 gcctcgcgga gcccgtaccg catcgtggcc gcccggcgga cgccgtgtac gagcagttcg
     1081 tgcgcgacgt gcttccctat ccgcccggca acatccatcc gcgcttctgg gggtgggtga
     1141 tcggcaccgg gacgccgctc gcgatgctcg ccgacctgct cgcctcggga atgaacccgc
     1201 agtcggcggg catccaggag ggcgcgcgct acatcgagga acgcgtcatc gcctggctcg
     1261 ccgagctgat gggattcccc cagggctcga gcggcctgct cgtctcggga ggctcgatgg
     1321 cgaacctcgt gggcctggcg gtcgcgcgcc aggcgaaggc ggggttcgat gtccgcgagg
     1381 ccggcatggg tgacggcgca ccggccctgg tcgtctatgc ctccacggaa gtgcacagct
     1441 cggtccgcaa ggcggtcgag ctgatgggcc tgggacggcg caacctgcgg ctcgtgcccg
     1501 tggacggcga gtatcgcatc gatgtgcagc agctgcgcgc ggcgatcgcc cgcgaccgcg
     1561 ccgagggccg ccggccgatc tgcgtggtcg gcaacgccgg caccgtgaac accggggcga
     1621 ccgacgacct ccgcgcgct
//
LOCUS       DBNGZW010000097         6079 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1168826, whole genome shotgun sequence.
ACCESSION   DBNGZW010000097 DBNGZW010000000
VERSION     DBNGZW010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6079)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6079)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6079
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1168826"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1236
                     /locus_tag="AC16JC_02445"
     CDS             <1..1236
                     /locus_tag="AC16JC_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163587.1"
                     /translation="GRDELRGAVDSATADARRLDLVAAVNAAAQVVGAEPAMVREVQI
                     VSDLQRTALAAGRVTLPAGVRVQVLAARSAPANRGIASARVTAGAVSVSIDGATPGGA
                     VPVTLRVRNRDVGRALATTGATVTLPVGVTGPGWWVGEVALPPDELRSDDRRTVVWHA
                     TPPVRVTVAPGAGSFVAAALEVLRSAGRVAEGTDVTIGDVRGSRSSVVSPPADPALLG
                     QVNRELAARGMAWRFGPTGTPGLIAGAGLDGVPVTRRQRLEGSDTGAILATVNGEPWV
                     VASKGIVLLGSRLDTSWTALPTAPVFVPFVDALVNRYARGEAAVEERVGAVHVAFRTI
                     GADTVGATVWGPDPRESDLTPAEPALIRQSLGAEIVEDSDFGLAAFAGLHRADISGVL
                     LAFALLLALGELAAATLAR"
     gene            1236..4511
                     /gene="mfd"
                     /locus_tag="AC16JC_02450"
     CDS             1236..4511
                     /gene="mfd"
                     /locus_tag="AC16JC_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HIR5163588.1"
                     /translation="MSLEFLLDRLDEIPATRAIAERLPGAGGRVAVGGLPGSSPAVLV
                     AALARRLPQRLFVVVAPAPADAERWLADLQALCGGEDGVALYPQRESLGLEEPHVEIA
                     GERVETLQALLSGAARVVVTTARASAERTAVPAALARARLDLTPGAARDSTLSTVVKR
                     LIEMGLERVPLVVEVAQFSVRGGILDVYAYGMAAPGRVEWWADEIVSIRSFDLDTQRS
                     GEVLERVTVLPVRSEAGAVDGEEPVRSTLLELLPSDTVLVLDQEPSVEREVDRAWSEA
                     AHHVEVARRMGADAPPREQQLVAPSDWTAAVRRFGRFVPGVGAEGTDLRFPLAPPEAI
                     DRDLKRLRQVVASAPPTVILCDNEGQLERLEDLLGEHAGGATLVVGALDAGFVLPALR
                     VLTDHEIFRRARRLRRPRRYRTSTSAAASRPLAPGDFVVHLEHGIGVFRGLQTIPVGE
                     ATLEVAVVEYEGGDRLNVPLYRLDQLEPFRSPGDGDAPPPRLHRLGGTSWQRQRDKTR
                     AAIRVMTQELLALYARRQLAAGFAFPRDTQWQRELESAFLYEDTPDQVRATVDVKRDM
                     ERARPMDRLLVGDVGYGKTEVALRAAFKAVQAGKQVAVLVPTTILAEQHGRTFRERLA
                     DYPVRLEVLSRFRSTRESAAALARLVDGSLDVVIGTHRLLSPDVSFKDLGLVIVDEEH
                     RFGVKHKERLKALKLSVDVLTLTATPIPRTLHLSLAGLRDLTLLETPPKDRSPVLTFI
                     EPWDDGLLEEAMARELDRGGQVYFVHNRIETIDTIAARVGALAPRARLAVAHGQMKEA
                     ELDRVMARFVAGEVDVLVCTMIVESGLDVPNANTMIVDRADRFGLAQLYQLRGRVGRS
                     HRRASCYLLVPEPVDPAAEERLAVLEHHTDLGSGYRIALRDLELRGAGNLLGAEQSGQ
                     AQAVGFDTYLRWLEEEVRTLKEGGAERGPLPAPEVVFDEAAHLPDDYVADHASKLDVY
                     RRLARAVRPGEIPAVREELRDRFGPLPPEAERLLLVTELRALGARAGLETILVRGDEA
                     RLTFRRDARPRMAGLTAALDAVQFAADVRRTVPLSLRLRRLGGEAIGPGLVRALTAVS
                     ADL"
     gene            4531..6015
                     /locus_tag="AC16JC_02455"
     CDS             4531..6015
                     /locus_tag="AC16JC_02455"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5163589.1"
                     /translation="MRRLAFVAVGFALTGCTAIRDAFTAHPDAAARAAGQTLSVERLA
                     EIAARAKGFPLQPPNLSKLAGVYVDFTLFAMAMADGQSLSDTAIVAEAMWPAVSQLKF
                     SHFHDRISADRMPNDRQIDSLFAAGELRAFQHLLITVPQSAAPTVVQQKEAQANALWR
                     SLVATGGTNFEAVARRSSEDPGSKAAGGWLGVRPRGQFVAPFEDAAWQLSPGAMTGVV
                     RTPFGFHIIRRPPLAEIRDSFAQGARDALLMRFDSSYLANLSSTRHVRVSSGAGEAVR
                     SATQDLDAAAHSNKSLVSYDGGEFRMKDLVRWLYGIDPRYLGSIPQASDSAIGELLQR
                     LTERHLALRQADSAKVQLTDSEWVAIRTEYDSSLAILGSELRFTPAMLHDSATTKEGR
                     ERFMMARVNDYFDRLVGGQAQFFPVPPLLAQLLRERSKWSIDESGVQRAVERAVALRA
                     SADSLRPPTAGGPGGEVRPAPGPPPVPPVPDSVLRKAPVRRTVQ"
BASE COUNT          830 a         1980 c         2301 g          968 t
ORIGIN      
        1 gggcgcgacg agctgcgtgg cgccgtggac agcgccacag cggacgcgcg acgcctggac
       61 ctcgtggctg cggtgaacgc cgcagcccag gtcgtaggcg ccgaacccgc aatggtacgc
      121 gaagtccaga tcgtgagcga tctgcagcgc accgccctgg cggccggccg ggttacactc
      181 cctgccgggg tcagggtgca ggtgcttgcc gcccgttcgg cgccggccaa tcgaggcatt
      241 gcctcggcac gggtcacggc cggggcggtg tccgtcagta tcgacggcgc cactcccggc
      301 ggtgcagtgc cggtcacact gcgcgtgcgc aaccgcgatg tggggagggc actggcgacg
      361 accggcgcca cggtcacgct tcccgtgggt gtcacgggac ccggttggtg ggtgggggag
      421 gtcgcgctgc cgcccgatga gctgcgcagc gatgaccggc gcactgtcgt gtggcacgcc
      481 acgccgccgg tgcgggtgac ggtcgccccg ggcgccggct cgttcgtggc ggcggcgctc
      541 gaggtgctgc ggtcggcggg gcgggtcgcc gaagggacgg acgtaacgat cggggacgtt
      601 cgggggagcc ggtcctccgt ggtctcccct cctgccgatc cggcgttgct cggtcaggtg
      661 aatcgcgaac tcgcggcgcg aggtatggcg tggcgattcg ggccgacggg gacaccgggc
      721 ttgatcgcgg gagccggact ggacggcgtg cccgtcacac ggcgccaacg actcgaggga
      781 tccgacacag gggcgatcct ggccacagtg aatggtgagc cctgggtggt ggccagcaag
      841 ggaatcgtgt tgctgggaag tcgcctggac accagctgga cggctttgcc cacggcgccg
      901 gtcttcgtgc cgttcgtcga cgccctcgtg aaccgctacg cccgcggcga ggcggccgtg
      961 gaggagcgcg taggtgcggt ccacgtcgcc ttccggacca tcggcgccga taccgtgggg
     1021 gccacggtct gggggccgga tccacgggaa tccgatctca caccggccga accggcgctc
     1081 atccgacagt cgctgggtgc cgagatcgtc gaggacagcg atttcgggct ggccgcgttc
     1141 gccggactgc atcgcgccga catcagcggg gtgttgctgg cgtttgcgtt gctgctcgct
     1201 ctcggagagc tcgcggcggc gacactggcg cgctgatgtc gctcgaattc ctgctcgatc
     1261 gtctggatga gatccccgcg acccgcgcca ttgcggagcg cctgccgggt gcaggcggcc
     1321 gggttgcggt gggggggctt cccgggtcca gcccggccgt cctcgtcgct gcgctcgcgc
     1381 gacggttgcc gcaacgcctg ttcgtcgtag tggcgccggc cccggccgat gccgaacgct
     1441 ggctggccga cctgcaggcc ttgtgcggcg gggaagacgg cgttgcgctc tatccgcagc
     1501 gggagagcct gggcctcgag gagccgcacg tcgagatcgc gggcgagcga gtcgagaccc
     1561 tgcaggcgct cttgtcgggt gcggctcgtg tcgtggtgac gacggcacgg gcgagcgcgg
     1621 agcggacggc ggtgcctgct gcactggcgc gcgcccgtct ggatctcacc cccggagcag
     1681 cgcgggactc gacgctgtcg acggtggtaa agcggttgat tgagatgggg ctggaacggg
     1741 tgccgctggt ggtggaggtg gcccagttca gcgtgcgagg cgggatcctc gacgtctacg
     1801 cgtacgggat ggccgccccg gggcgggtcg agtggtgggc cgacgagatc gtttcgatcc
     1861 gatccttcga tctggatacg cagcggtcgg gagaggtgct ggagcgggtg acggtactgc
     1921 ccgtccgttc ggaagcgggc gcggtggacg gagaagagcc cgttcgctcg acgctgctcg
     1981 agctgcttcc gagcgatacg gtcctcgtgc tggaccagga gccgagcgtc gagcgcgaag
     2041 tggaccgggc gtggtcggaa gccgcgcatc acgtggaggt tgcccggcgg atgggcgcgg
     2101 atgctccacc acgggagcag cagctcgtcg ccccgtcgga ttggacggcg gccgtgcggc
     2161 ggttcgggcg gttcgtgccg ggagtcgggg ccgaaggcac ggacctgcgt ttcccgctgg
     2221 cacctcccga agcgatcgac cgagacctca agcggctgcg tcaggtcgtg gcgtctgcac
     2281 caccgaccgt catcctgtgc gacaacgagg gacagttgga gcgcctcgag gacctgctgg
     2341 gcgagcacgc cggcggggcg acgctcgtgg tgggggcgct ggacgcgggg ttcgtgctgc
     2401 cggcgctgcg ggtgctcacg gatcacgaga tcttccgtcg tgcccgccgt ctgcggcgtc
     2461 cgcggcgtta ccgcacgtcg acgtccgcgg ccgcgtcccg gccgctcgcg ccgggcgact
     2521 tcgtcgtcca tttggagcac ggcatcggag tttttcgcgg actgcagacc atccccgtgg
     2581 gcgaggccac gctcgaggta gccgtcgtcg aatacgaagg tggcgaccgg ctcaatgtgc
     2641 cgctgtaccg gctggaccag ctcgagccgt ttcgctcgcc gggagacggc gacgcgccgc
     2701 cgccgcgcct gcaccggctc ggcggtacca gctggcagcg gcagcgtgac aagacccggg
     2761 ccgcgatccg cgtgatgacg caggagctgc tcgcgctgta cgcgcgacgg cagctcgcgg
     2821 ccggattcgc cttcccccgg gacactcagt ggcagcgcga gctggagtcg gcgttcctgt
     2881 acgaggacac ccccgaccaa gtgcgggcga ccgtcgacgt gaagcgcgac atggagcgcg
     2941 cccggccgat ggaccggctc ctcgtgggtg acgtggggta cggcaagaca gaggtcgcgc
     3001 tgcgcgccgc cttcaaggcg gtccaggcgg gcaagcaagt cgccgtgctg gtgccgacga
     3061 cgattctggc ggagcagcac gggcggacgt tccgggagcg gctcgccgac tatcccgtgc
     3121 gactcgaggt gttatcgcgg ttccgttcta cccgcgagag cgcggcggcg ttggcgcggc
     3181 tggtggacgg atcgctcgac gtggtgatcg gcacgcaccg gttgctctcc ccggatgtgt
     3241 cgttcaagga tctcggcttg gtgatcgtgg atgaggagca ccgcttcggg gtgaagcaca
     3301 aggagcgcct gaaggctctg aaactgagcg tcgacgtgct gacgctgacg gcgacgccga
     3361 ttccccgcac gctgcacttg tcgctcgccg gactgcgtga cctgacgctg ctggaaacgc
     3421 cgcccaaaga tcgttcgccg gtgctcacgt tcatcgaacc gtgggacgac gggctgctcg
     3481 aggaggccat ggcccgggag ctcgatcgcg gcggccaggt gtacttcgtg cacaaccgca
     3541 tcgagacgat cgataccatc gcggcccggg tgggcgccct ggcgccgcgc gcgcgcctgg
     3601 ccgtggcgca cggccagatg aaggaagcgg aactggaccg cgtgatggcc cgcttcgtgg
     3661 cgggtgaggt ggatgtgctg gtgtgtacca tgatcgtcga gtcggggctc gacgtgccga
     3721 acgccaatac catgatcgtg gaccgggcgg accgcttcgg cctggcgcag ctctatcaat
     3781 tgcgcggccg cgtgggacgg agtcaccggc gggcttcctg ttatctcctc gtccccgagc
     3841 cggtcgatcc ggcggcggag gagcgcctcg cggtgctcga gcatcatacg gatctgggtt
     3901 cggggtaccg gatcgccctg cgcgacctgg agctgcgcgg ggcgggcaat ctgctgggcg
     3961 ccgaacaatc cggccaggcg caggcggtcg gcttcgatac ctatctgcga tggctcgagg
     4021 aagaagtgcg gacactcaag gaaggcggtg cggagcgcgg tccgctcccc gcccccgaag
     4081 tggtcttcga cgaagccgcg cacctgccgg atgactacgt cgccgatcat gcgtcgaagc
     4141 tggacgtgta ccggcgactg gcgcgcgcgg tgcggcctgg cgagattcca gcggttcgcg
     4201 aggagctgcg cgaccgtttc ggccctctgc ctcccgaggc ggagcgactg ctgctcgtga
     4261 cggaactccg cgcgctcggc gcccgggcgg gattggaaac gatcctggtg cggggcgacg
     4321 aggcgcggct cacgtttcgg cgggacgcac ggccccgcat ggccggcctc acggcggcgc
     4381 tcgacgcggt gcagttcgcc gccgacgttc gccggacagt tccgctctcg ctgcgactcc
     4441 ggcgcctggg gggcgaggcg atcgggcccg ggctggtgcg ggcactcacg gcggtatccg
     4501 ccgatctctg acctggatag gagtcctggc atgcgtcgac tggcgttcgt ggcggtgggg
     4561 ttcgcgttga ccggttgcac ggcgattcgg gacgcgttca cggcccatcc tgatgccgcg
     4621 gcgcgcgccg ccggccagac cctcagcgtg gagcgcctcg ccgagatcgc cgcgcgggcc
     4681 aaaggcttcc cgctacagcc gccgaacctg agcaagctgg ccggcgtgta cgtggacttc
     4741 acgctgttcg ccatggccat ggccgacggc cagtcgctca gcgacaccgc gatcgtcgcg
     4801 gaggcgatgt ggcccgccgt gtcacagctg aagttctccc attttcacga ccgcatcagc
     4861 gccgaccgga tgccgaacga ccggcagatc gattcgctgt tcgccgccgg ggagctgcgg
     4921 gccttccagc atctgctcat taccgttccc cagagcgccg ctccgaccgt ggtgcagcag
     4981 aaggaggccc aggccaacgc cctgtggcgc tccctcgtcg cgacgggggg aacgaatttc
     5041 gaggcggtgg cgcggcgcag ctcggaggat cccgggtcga aggcggcggg cggctggctc
     5101 ggcgtgcggc cgcgggggca gttcgtggcg ccgttcgaag acgccgcgtg gcagctgtca
     5161 cccggcgcga tgacgggcgt ggtacgcaca ccgttcggtt tccacatcat tcgtcgtcca
     5221 ccgctggcgg agattcgcga cagcttcgcg caaggcgccc gggacgcgtt gctgatgcgg
     5281 ttcgattcct cgtatctggc caatctctcg agcacacggc acgtgcgggt gtccagcggg
     5341 gcgggcgagg ccgtgcgatc cgccacgcag gacctggacg ccgcggccca cagcaacaag
     5401 tcgctggtca gctacgacgg cggcgagttc agaatgaagg acctggtgcg ctggttgtac
     5461 ggcatcgatc cccgctatct cggcagtatt ccacaggcga gcgattcggc gatcggggag
     5521 ctgctgcagc ggctcacgga gcggcatctg gcgttgcggc aagcggactc cgccaaggtg
     5581 cagctcaccg actcggagtg ggtggcgatc cggaccgagt acgattcctc gctcgccatc
     5641 ctgggctccg agctgcggtt cactcccgcc atgctgcacg attcggcgac gacgaaggaa
     5701 gggcgggagc gcttcatgat ggcgcgcgtg aacgactact tcgaccggct ggtgggtggg
     5761 caggcgcagt tcttccccgt cccaccgctg ctcgcgcagc tgctgcgcga gcgcagcaag
     5821 tggtcgatcg acgaatccgg cgtgcagcgg gcagtggagc gggcggttgc gctgcgggcc
     5881 agcgccgact cgctgcgtcc gcccacggcc ggtggtcccg gtggggaagt ccggccggct
     5941 cccggacctc cgcctgtccc gccggttccc gactcggtgt tgcgcaaggc gccggtgcgg
     6001 cgcacggtgc aatgatccga cccggccggc atgcgctcct cgcgctgttg ctcggagcga
     6061 ccgcggctcc cgttgcgtg
//
LOCUS       DBNGZW010000098         2464 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1644821, whole genome shotgun sequence.
ACCESSION   DBNGZW010000098 DBNGZW010000000
VERSION     DBNGZW010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2464)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2464)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2464
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1644821"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..403
                     /locus_tag="AC16JC_02460"
     CDS             <1..403
                     /locus_tag="AC16JC_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5163590.1"
                     /translation="AKLFAGALAAGAPAAARTHTRRAARAFGARLGREARRRAGTRAG
                     RARLLAQLQTVLREHGYEPCGGETASEAIRLRNCPFDALARDYRPLMCGMNQALLAGV
                     VRGLGLRGLEAAADPQPGMCCVVLRPLPRRS"
     gene            complement(451..1446)
                     /locus_tag="AC16JC_02465"
     CDS             complement(451..1446)
                     /locus_tag="AC16JC_02465"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR5163591.1"
                     /translation="MVARPLLFSAGLATLMGAACAWQRPAPSVVTTPMDDHLMGMSAA
                     SMAAPRIIPRAETQGTAGLPPSASTAAARLAASPRHAEWVKIPWGPGSTDSLMAWIVY
                     PVTSRPHTPVVVVVHEIFGLSTWVRGVADQVAADGFIAIAPDLVSRARGGPSTDELPS
                     DSAVKLIRGVSSAERNLGVAAAARYAMSQPSAAQRYGVIGFCWGGSTTWMHAINQGVT
                     GFSGAVPFYGLPYMNGPVPDADSLAKIRVPVMLMNGAQDARIGAAMPAVDSIMRALHK
                     DYVGTNYENAIHGFARAQDDPRTPRDSVVEAANLAALKDAWPRTTAFLRKHLGLD"
     gene            1624..2451
                     /locus_tag="AC16JC_02470"
     CDS             1624..2451
                     /locus_tag="AC16JC_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011510650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HIR5163592.1"
                     /translation="MATTPDPRALERSRIFRRLHESGCFVIPNPWDRGSAILLAQLGF
                     PALATTSSGFAWSLGRQDNHVSLEQALAHYRAIAEAVTVPVNADFEGGFAIAPAEVAV
                     NVAAAAATGIAGLSIEDSTGDAASPLFEFPLALERVRAARHAIDESGTGVLLTGRSEG
                     FVVGRPDLTETIRRLTAYAEAGADCLFAPGVRATADITAIVRAVAPKPVNVVVGGDYT
                     TVAEMAQLGVRRISVGGSLARTAWTGFLAAAREIAEQGTFSGLARAIPGADMNRFFA"
BASE COUNT          356 a          869 c          895 g          344 t
ORIGIN      
        1 cgcgaagctc ttcgccggcg cgctcgccgc cggcgcgccc gcggccgcgc gcacccacac
       61 ccgccgggcg gcgcgcgcgt tcggcgcccg cctcggtcgg gaagcccggc gtcgcgccgg
      121 cacccgagcc ggccgcgcgc ggctgctggc tcagctccag acagtgctgc gcgagcacgg
      181 ctacgagccc tgcggcgggg agaccgcgag cgaggcgatc cggctccgca actgtccctt
      241 cgacgcgctg gcgcgggact atcgcccgct gatgtgcggg atgaaccagg ccttgctggc
      301 tggcgtggtg cgggggctcg gactacgggg gctcgaggcc gcggcggatc ctcagcccgg
      361 aatgtgctgc gtcgtcctgc gaccgctgcc gcgacgcagc taaggatact aagaccaacg
      421 gcaagatcgg ctatctcgag cgcccggccc tcagtcgaga ccgaggtgct tcctgaggaa
      481 cgccgtggta cgcggccagg cgtccttgag cgccgcgagg ttcgccgctt cgaccaccga
      541 atcgcgcggg gtccggggat cgtcctgggc ccgggcgaag ccgtggatgg cgttctcgta
      601 gttggtgccg acgtagtcct tgtggagcgc ccgcatgatc gagtcgacgg cgggcatggc
      661 ggcgccgatg cgggcgtcct gcgcgccgtt catcagcatg accggcaccc ggatcttcgc
      721 cagcgagtcg gcgtcgggta cggggccgtt catgtacggc aggccgtaga acgggaccgc
      781 gccggagaaa ccggtcacgc cctggttgat ggcgtgcatc cacgtggtgg accctcccca
      841 gcagaagccg atcacaccgt agcgctgggc cgccgagggt tgggacatcg catagcgggc
      901 ggcggcggca acgccgaggt tgcgttccgc ggagctgacg ccgcgaatca gcttgaccgc
      961 cgagtcggac gggagctcgt cggtgctcgg accgccccgc gcgcgtgaga cgagatcggg
     1021 cgcaatcgcg atgaacccgt ccgccgccac ctgatcggcc acgccgcgca cccatgtcga
     1081 cagcccgaag atctcgtgta cgacgactac caccggtgta tgggggcgcg aggtgaccgg
     1141 gtacacgatc cacgccatca gcgagtcggt cgagccgggc ccccagggga tcttcaccca
     1201 ctccgcgtgc cgcgggctgg ccgcgagccg cgccgccgcc gtgctggcgc tggggggcag
     1261 tcccgcggtc ccctgcgtct cggcgcgtgg gatgatccgc ggcgcggcca tggaagcagc
     1321 cgacatcccc atgagatgat cgtccatggg cgtcgtgacc acggagggtg cgggccgttg
     1381 ccacgcacag gcggctccca taagggtggc cagacccgcg gagaagagga gagggcgtgc
     1441 aaccatggat cgctccggag cgtgttgatg tcgaatgaac acgctactct ccgcccacgc
     1501 cccgcggaag acgaggggtg acgccggcga acgtcaggaa gcggcaagga tggcggagcc
     1561 cgactgcgcg gaagactata gattggggca acgatcacga tacccgctgc gtgcgtccct
     1621 gacatggcca ctactcccga cccccgcgcc ctcgagcgca gccgcatctt ccgccggctg
     1681 cacgagtccg gctgcttcgt gatccccaac ccgtgggacc gtggtagcgc gatcctgctc
     1741 gcccagctcg gcttcccggc gctcgccacc accagctccg gattcgcctg gtcgctcggc
     1801 cggcaagaca accacgtatc tctggagcag gcgctggcgc actaccgcgc gatcgccgag
     1861 gcggtcacgg tgccggtcaa cgcggacttc gagggcggct tcgcgatcgc ccccgcggag
     1921 gtcgccgtca acgtcgccgc cgcggccgcc acggggatcg cgggtctctc gatcgaggac
     1981 tcgaccggcg acgcggcgag tccgctgttc gagttccccc tggccctcga acgggtgcgc
     2041 gcagcccggc acgccatcga cgagagcgga acgggcgtcc tgctcaccgg ccggtccgag
     2101 gggttcgtgg tgggccgacc ggacctcacc gagacgatcc ggcggctcac cgcctacgcc
     2161 gaagcgggcg ccgactgtct gttcgctccc ggggtgcgcg ccacggccga catcacggcg
     2221 atcgtgcggg cggttgcgcc caagccggtc aacgtcgtgg tgggcggcga ctacacgacc
     2281 gttgccgaga tggcgcagct gggcgtgcgc cgcatcagcg tcggcggctc gctggcccgg
     2341 acggcctgga ccgggtttct cgcggcggca cgggagatcg ccgagcaggg gacgttctcg
     2401 ggcctcgccc gtgccatccc cggggcggac atgaatcggt tcttcgcctg aaggcagcgg
     2461 cggc
//
LOCUS       DBNGZW010000099         3274 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_281108, whole genome shotgun sequence.
ACCESSION   DBNGZW010000099 DBNGZW010000000
VERSION     DBNGZW010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_281108"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..>3274)
                     /locus_tag="AC16JC_02475"
     CDS             complement(213..>3274)
                     /locus_tag="AC16JC_02475"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5163593.1"
                     /translation="TVAGHPDVARIGPEGGAEQIWTADADGSNAKELTKSGSNLYASP
                     EWTPDGNYIVASRTTGVLGSVYELWLYHKDGGSGAAMIKSGPPPAPGGSPFSGPGWNT
                     LGAAFGSDPRYVWVSRHRGGFGYDLDFPLWQVAIYDRQTGKTFTQTDLYGSAMRPIPS
                     PDGKWLVYATRYDTETGLRLRNLATGDEKWLAYPVTRDDQESRFTRDLEPGSAFTPDA
                     KALITSYGGKIWRIEIPSGTATAIPFTAHVEQRLGPLVHFVNHVDTGAVQARQIRDTR
                     LSPDGRRIVFSALDRLYVMDLAGGTPRRLTDDSVHEQAPAWSPDGQWVAYVTWTSAQG
                     GALMKVRADGKARTRLVADPAFFDSPVWSPDGQRVVAVKGPRQARINERFGPGYDLVW
                     VPAAGGAVTRIGPYDGRGRPQFSRNPDRIFYYAGSDGLVSVRWDGTDRQAYIKVNGYT
                     YPGPGAQPDPADEIIVAPDTDRVLALQGNNVYLVTLPQVGGETPAINVSTPASASFPV
                     KRLTRIGGDFIGWSADGKMAIWSIGASFFRWDPAVADSAEKAKAKTDSIRADSLKKAQ
                     ADSTKPKPDSAAKARVDSLAKLPADEGLRTDVVIRAARDVPRGTVVLRGARLITMKGE
                     EVIERGDIVVTDDRIVWVGPQGGGTVPAGARIIDVTGKTIIPGLVDVHAHPWATFGVH
                     QTQVWKYLANLAWGVTTVRDPQTATTDVLTYADQIETGELLGPRLYHTGPGVFWDENF
                     QNLDEARNALKRYSEFYHTHWIKQYMTGNRKQRQWVIMAAQELGLMPTTEGGLDFKMN
                     LTEMLDGYPGHEHSYPLMPLYQDAIGLAAQSGITYTPTLLVNYGGPFGEDYYFEHYNG
                     HDDPKMRRFVPHEVLDERFLRRQWFRDDQYVFNRIAAAAGAILRAGGNVGMGCHGEMD
                     GIGCHWELWGFAAGGLSPLEVLRIGTIDGAKGLGLDTDLGSLEPGKFADLIVLDGNPL
                     EDIHNTNAIHYVMKNGRLYEGATLNEVWPRQKALPTMWWWGEEPKTR"
BASE COUNT          462 a         1151 c         1098 g          563 t
ORIGIN      
        1 gaccgtgcgt gtgttcagcg ccggcgcccg ctcctgagcg ggaccgtcct gcggaaggat
       61 gccagctcgc ggttcttgac taaggacgga agactaacta cgtcgagcga aggcgtcaag
      121 cgagaacgtc gagcaaagac gtcgagcaaa ttcgtgcgct cttgcctgac gtctctgctt
      181 gacgcctctg cttgacgcct ctgcttgacg ccttacctgg tttttggctc ctctccccac
      241 caccacatcg tcggcagcgc cttctgccgc ggccacactt cgttgagcgt cgcgccctcg
      301 tacaggcgcc cgttcttcat cacgtagtgg atcgcgttgg tgttgtggat gtcctcgagc
      361 ggattgccgt cgagcacgat cagatccgcg aacttgccgg gctcgagtga ccccagatcg
      421 gtgtcgagtc ccagtccctt ggccccgtcg atggtcccga tgcgcagcac ttcgaggggc
      481 gacagcccgc ccgccgcgaa tccccacagc tcccagtggc agccgatgcc gtccatctcg
      541 ccgtggcagc ccatccccac gttcccgccg gcccgcagga tcgcccccgc cgcggcggcg
      601 atgcggttga acacgtattg atcgtcgcgg aaccactggc gccgcaggaa ccgctcgtcc
      661 aggacttcgt gcggcacgaa gcgccgcatc ttcggatcgt cgtgcccgtt gtaatgctcg
      721 aagtaatagt cctcgccgaa ggggcccccg tagttcacga gcagcgtcgg cgtgtaggtg
      781 atccccgact gggccgcgag tccgatcgcg tcctggtaca acggcatgag gggatagctg
      841 tgctcgtggc cgggatagcc gtccagcatc tccgtcagat tcatcttgaa gtcgagcccg
      901 ccctcggtcg tgggcatcag gcccagctcc tgcgccgcca tgatcaccca ctgccgctgt
      961 ttgcggttcc ccgtcatgta ctgcttgatc cagtgggtgt gatagaactc cgagtaccgc
     1021 ttcagcgcgt tgcgcgcctc gtcgaggttc tggaagttct cgtcccagaa cacgccgggg
     1081 ccggtgtgat agagccgtgg cccgagcagc tcgccggtct cgatctggtc ggcgtaggtc
     1141 aggacgtcgg tcgtggcggt ctgcggatcg cgcaccgtcg tgacgcccca cgcgaggttc
     1201 gccaggtact tccacacctg cgtctggtgg accccgaacg tcgcccaggg gtgcgcgtga
     1261 acgtccacga gcccggggat gatcgtcttg cccgtcacgt cgatgatgcg cgcgcccgcc
     1321 ggcacggtcc ccccaccctg ggggccgacc cacacgatgc ggtcgtcggt cacgacgatg
     1381 tcgccccgct cgatgacctc ctcgcccttc atcgtaatga ggcgggcgcc gcgcagtacc
     1441 accgtgccgc ggggcacgtc gcgcgccgcc cggatcacga cgtcggtgcg cagcccttcg
     1501 tcggcgggca gcttggcgag cgagtccacc cgcgccttcg ccgccgagtc cggcttcggc
     1561 ttggtggaat ccgcctgcgc cttcttgagc gagtcggccc ggatggagtc ggtcttcgcc
     1621 ttggccttct ccgccgagtc ggccacggcg ggatcccagc ggaagaagct cgcgccgatg
     1681 gaccagatcg ccatcttgcc gtcggcgctc cacccgatga agtcgcctcc gatccgggtc
     1741 aggcgcttga ccgggaacga cgccgaggcc ggagtcgaca cgttgatcgc cggcgtctcg
     1801 ccgcccacct ggggcagcgt cacgagatag acgttgttgc cctgcagggc gagcacgcgg
     1861 tcggtgtcgg gcgcgacgat gatctcgtcc gccggatcgg gctgggcgcc cgggccggga
     1921 taggtgtagc cgttcacctt gatatacgcc tgccggtcgg tgccgtccca gcgcaccgag
     1981 accagcccgt cgctcccggc gtagtagaag atccggtccg gattccgcga gaactgcggg
     2041 cgcccccgcc cgtcgtaggg gccgatccgc gtgaccgcgc cgcccgcggc gggtacccac
     2101 accagatcgt agcccgggcc gaagcgctcg ttgatgcgcg cctgacgcgg ccccttcacg
     2161 gccaccaccc gctggccgtc cggcgaccat accggcgagt cgaagaaggc cgggtcggcg
     2221 acgagccggg tccgcgcctt gccgtcggcg cgcaccttca tgagcgcgcc gccctgggcg
     2281 gaggtccacg tcacgtaggc cacccactgc ccgtcgggcg accaggcggg cgcctgctcg
     2341 tgcaccgaat cgtcggtgag ccgccgcggc gtccccccgg ccaggtccat cacgtacagc
     2401 cggtcgagcg cgctgaacac gatgcggcgc ccgtcgggtg agagccgggt gtcgcggatc
     2461 tggcgggcct ggacggcgcc ggtgtcgacg tggttgacga agtgcaccag cggcccgagg
     2521 cgctgctcca cgtgcgcggt gaacggaatg gcggtcgccg tgcccgaggg gatctcgatg
     2581 cgccagatct tgccgccgta ggaggtgatc agcgccttcg cgtccggcgt gaacgcgctg
     2641 ccgggctcca ggtcgcgggt gaaccgcgat tcctgatcgt cccgcgtcac cgggtaggcg
     2701 agccatttct cgtccccggt cgccaggttg cgcagccgca gtcccgtctc cgtgtcgtag
     2761 cgcgtcgcat agaccagcca ttttccgtcg ggcgacggga tcggccgcat cgcgctgccg
     2821 tagagatcgg tctgcgtaaa ggtcttgccc gtctggcggt cgtagatcgc aacctgccac
     2881 agcgggaagt cgagatcgta gccgaagccg ccacggtggc gggacaccca gacgtagcgc
     2941 ggatcggacc cgaaggcggc gccgagcgtg ttccagccgg ggcccgaaaa cgggctgcca
     3001 cccggcgccg gcggtggacc ggacttgatc atcgccgcgc ccgacccgcc gtccttgtgg
     3061 tacagccaca actcgtacac gctgcccagc acgccggtcg tgcgcgacgc gacgatgtag
     3121 ttgccgtcgg gcgtccactc gggggaggcg tagaggttgc tgcccgactt agtgagctcc
     3181 ttggcgttgc tcccgtcggc gtcggccgtc cagatctgct cggcgccgcc ttcgggtccg
     3241 atccgcgcta cgtctgggtg tcccgccacc gtgg
//
LOCUS       DBNGZW010000100         1706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1710299, whole genome shotgun sequence.
ACCESSION   DBNGZW010000100 DBNGZW010000000
VERSION     DBNGZW010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1710299"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1099
                     /locus_tag="AC16JC_02480"
     CDS             <1..1099
                     /locus_tag="AC16JC_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163594.1"
                     /translation="LQLAKGALVPSGVFEDSSAWTGSSGAVRLLEIQGGVVDGKYALV
                     SRVGVPEPRKPADGRHAITLSRLSDDEFRWDTTVDFAIGSIRPAEVARVLARLASSAE
                     GKTEREVRADLRTAAPRTAWALGQLFSLDSVAPTRLGDGSTAVTLVVGIHSDRLTRRY
                     AAFGDYVRRYVDPVRYRVVVSDRAGVPYFEAVARDQVMTVRLRTCNGGLVPLSGPIRP
                     LPDTLALLVDFKAKVKHLGVGFHGLRMDLVHVRHDEAENSWVVTAHHEPEWDFPLATA
                     RLLRAPLRRPFSGEGSLFRIGVRGDGTGQTVIVRQVRLFVQESAILRFLNSLSGAAFS
                     EFEDRVDREENAWLREVFAGMRADARAAMGT"
     gene            complement(1281..1353)
                     /locus_tag="AC16JC_02485"
     tRNA            complement(1281..1353)
                     /locus_tag="AC16JC_02485"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1318..1320),aa:Ala,seq:cgc)
BASE COUNT          277 a          554 c          615 g          260 t
ORIGIN      
        1 cctgcagttg gccaaaggcg cactggtgcc ttcgggcgtg ttcgaggaca gcagcgcgtg
       61 gactggaagc tctggggccg tccgcctgct cgagatccag ggcggagtgg tggacgggaa
      121 atacgcgctc gtgtcgcggg tgggtgtgcc cgaaccgcgg aagccggccg acggccgaca
      181 cgcgatcacg ctgagccgac tgtcggacga cgagttccgg tgggacacca ccgtggactt
      241 cgccatcggg agcatccggc ccgcggaggt agcgagagtg ctcgcccgtc tcgcctcgtc
      301 ggccgaaggg aagacggaac gcgaggtcag agccgatctg cggaccgccg caccgcggac
      361 ggcgtgggcg ctcgggcagc tgttctcgct cgactcggtc gcgccgaccc ggctcggcga
      421 tggaagcacc gccgtgacgc tggtggtcgg catccactcg gaccgactca cgcggcggta
      481 cgcggcattc ggcgattacg tgcgccggta cgtggatccg gtgcggtacc gcgtggtggt
      541 ctccgaccgc gctggcgtgc cctatttcga ggcagtcgcg cgcgaccagg tgatgacggt
      601 gcgactgcgc acctgcaacg gcgggctcgt gccgctctcc ggcccgatcc ggccgctccc
      661 ggacaccctc gcgctcctgg tggacttcaa ggcgaaggtg aagcacctcg gagtggggtt
      721 ccacgggctc cggatggacc tggtgcacgt gcggcacgac gaggcggaga attcctgggt
      781 cgtgacggcg caccacgagc cggagtggga tttcccgctg gcgacggccc gcctgcttcg
      841 cgcgccgctc cgccgtccgt tcagcggcga gggctcgctg ttccgcatcg gcgtgcgcgg
      901 ggacggaacg ggccagacgg tgatcgtccg gcaggtgcgg ttgttcgtgc aggagagcgc
      961 gatcctgcgc ttcctcaact ccctgtcggg ggcggcgttc agcgagttcg aggaccgcgt
     1021 ggaccgggaa gagaatgcgt ggctgcgcga ggtgttcgcg gggatgcggg cggatgcgcg
     1081 ggcggcgatg gggacctgaa cgaagaacgc cgcgatcatc ggatcgcggc gtcgatctct
     1141 cgcgcgaggg cgagagccgt tcggggttgc cacacacgcc gtcattggaa ctctccgtcc
     1201 taccgaacgg cggcgggcaa tgtatcgaac tgttggcgga tcaagcgcaa gcctcgcgcc
     1261 aaccaattca cgtaacgaaa tggagctgag ggggatcgaa cccctgacct ctgcagtgcg
     1321 attgcagcgc tctcccagct gagctacagc cccgcattgg cgacggctgt tggagaacag
     1381 gcctccgccc ccatcagcca gacggaaacg gccccgcaat cgagcggagc cgccgtcact
     1441 gtaacaaatc tagccggacg gcccgcgctg tcaagcagac gcgccagcac gtgtgcgcgt
     1501 aacgagggcg caccggcgtg ggagcaagcg gtcgcgtgac gccgttctgg ccacatgacg
     1561 cacccgatcg gatcgcctta cgtgcgcgac cagatctcgc agcgcgccac cctgctcaac
     1621 gagaagcggc tccagcccga cggggagtac gtcctctact ggatgcagtc cacccagcgg
     1681 ttcgaggaca actgggccct gcgcta
//
LOCUS       DBNGZW010000101         4516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1100174, whole genome shotgun sequence.
ACCESSION   DBNGZW010000101 DBNGZW010000000
VERSION     DBNGZW010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1100174"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2779
                     /locus_tag="AC16JC_02490"
     CDS             <1..2779
                     /locus_tag="AC16JC_02490"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5163595.1"
                     /translation="PSDQPIFVLAVQGENWALRDLTRLADEVVSRRIENVYGVGSVTL
                     VGGLKREIHVQLQPDRLQALGISPDLVTAALRRENGDVPAGRVEQGNAEQLVRVAGKI
                     RDPKDFASLIVTSRNGIPVRLGQIARIEDAQEEARDVAYVNGNRAVALEVRKTSGANT
                     VEATDHIKAVMAELEPTLPHGVRLAVVQDNSTWIRNSVDDVQKTLLEGAFLTVLIVFL
                     FLNSWRSTVITGLTLPVSVIAAFLAIYAFGFTINIMTLMALSLAIGILIDDAIVVREN
                     IVRHVERGEDHYTAARKGTAEIGFAVMATTMSVIAVFVPVAFMKGIVGRFFFPFGITV
                     AFAVLVSLFVSLTLDPMLSSLWYDPQAEGGARRGPIGRALERFNNSFVALGQRYRKVI
                     AWALDHRLATMGIATVAFFAAMSLFDLGLVGGQFMPTSDNEQTLLTIETPVGSSVDYT
                     AGKALELSHYLDSLPEVALTYATVGGAQQNESVNKGQIYVKLTGAHSRARSQQQLEAD
                     LRRVLPRFQGVAARVSQIGAAGGSQAPIQLNLQGPALVRLQGLSDSALAAIRTVPGLV
                     DLRSSLEGRKPEFSVDVSRDLAADVGLSVGGIGAALRPVLTGEQAGTWEDETGLAHDV
                     VVRLVPESRQTESDLARIPLPSSRIDARTGAPVMIPLGQVATIRSSGAPNEIKRLDLE
                     RVATIEGNYQGRPLTDVVKDVRARLATLDLPPGYRFDFGGEQKDFAETVGYMLESLTL
                     AIVFIYLILASQFGSFLQPLAIMLSLPLSLVGVMLGLMLTRGTFNIMSMIGVIMLMGL
                     VTKNAILLIDFANKARESGSDRREALIDAGELRLRPIVMTTLAMIFGMLPTALALGAG
                     AEFRAPMAHAVIGGLITSTLLTLVVVPVVYSYLDDFGAWVGAKVKRWTSAPEPAPVPV
                     ERSFTEPALHQ"
     gene            2858..3115
                     /locus_tag="AC16JC_02495"
     CDS             2858..3115
                     /locus_tag="AC16JC_02495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor-like protein"
                     /protein_id="HIR5163596.1"
                     /translation="MPEQLVGTVSHYFKGPSVAIVRLTTGPLAVGDRIHFHGHTTDFT
                     EQITSMEVNHQKVERANVGEEVAIQVTDRAREHDQVLQVTD"
     gene            3129..3692
                     /locus_tag="AC16JC_02500"
     CDS             3129..3692
                     /locus_tag="AC16JC_02500"
                     /inference="COORDINATES: protein motif:HMM:NF049836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN-like domain-containing (lipo)protein"
                     /protein_id="HIR5163597.1"
                     /translation="MQRVILAVVVGCACVGESGDTGRGPPARSALAVQDTTAAFAGTL
                     EPVRRPSSSMRVHAVVLQAVRAGPEPGFDRVVFEFAGPGLPGYQVQYATGPIQACGSG
                     AVVPLAGMDHLLVRFEPAQAHDTLGRVTAADRMLAPRLPQVLDVKLVCDFEGQVEWAV
                     GVAARRPFRVREDSQPPRVIVDVRHRE"
     gene            3685..>4516
                     /locus_tag="AC16JC_02505"
     CDS             3685..>4516
                     /locus_tag="AC16JC_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163598.1"
                     /translation="MSDRRAALWIAGVALAACRQPAPTPQPCPQASPARLARTLNVDS
                     LPSRYDSQFTAAAAEFRVPRGMLKGIGWVETRWQMVRGEEEFPGRPPAFGVMALRGTA
                     LERGAALAGVSVQVARTDPLANIRAAAALLAAAADSAGIDRTKAEAWEPIVGRFSGIA
                     APYARAAYTLDVDRTLAPQERLNPQVTCPPPPPPPPPPPPPPPPPPPEPKPVWRPSPN
                     YNTRPQDSAGTVHMVIIHTCESGYTGCWGWLVNPASQVSAHYVVDEDGTNRKSTRLNS
                     S"
BASE COUNT          674 a         1549 c         1601 g          692 t
ORIGIN      
        1 cccgtccgat cagccgatct tcgtgctggc ggtgcagggg gagaactggg cgctgcgcga
       61 cctcacccgg ctggccgatg aagtggtgag ccgccggatc gagaacgtct acggggtggg
      121 cagcgtgacg ctggtggggg gcctcaagcg ggagatccac gtgcagctgc agcccgatcg
      181 gctgcaggcg ctcggcatct cgcccgacct ggtgacggcg gcactgcgcc gcgagaacgg
      241 cgacgtgccg gcgggccgcg tggaacaggg gaacgccgag cagctcgtgc gggtggcggg
      301 gaagatccgc gacccgaagg atttcgcctc cctgatcgtc acgtcgcgca acggcatccc
      361 ggtgcgcctg gggcagatcg cccgcatcga ggacgcccag gaggaggcgc gcgacgtcgc
      421 ctacgtgaac ggcaatcgcg ccgtggccct cgaggtccgc aagacgtcgg gcgccaatac
      481 ggtcgaggcg accgaccaca tcaaggccgt gatggcggag ctcgagccca cgctgccgca
      541 cggcgtgcgc ctggccgtcg tccaggacaa ctcgacctgg atccgcaatt cggtggacga
      601 cgtgcagaag acgctgctcg agggcgcctt cctcacggtc ctgatcgtct tcctattcct
      661 caattcgtgg cgctcgacgg tgatcacggg gctcacgctc ccggtgtcgg tcatcgccgc
      721 gttcctggcg atctacgcct tcggcttcac gatcaacatc atgacgctga tggcgctgtc
      781 gttggccatc gggatcctga tcgacgacgc catcgtggtg cgggagaaca tcgtgcgcca
      841 cgtggagcgc ggggaggatc actacacggc cgcccgcaag ggcaccgccg agatcggctt
      901 cgccgtcatg gcgacgacga tgtcggtgat cgcggtattc gtcccggtcg cgttcatgaa
      961 aggcatcgtg gggcggttct tcttcccgtt cgggatcacg gtggccttcg ccgtgctcgt
     1021 gtcgctgttc gtgtcgctca cgctcgaccc gatgctctcg tcgctgtggt atgacccgca
     1081 ggcggagggt ggggcgcggc gcggaccgat cgggcgggcc ctggagcggt tcaacaactc
     1141 gttcgtagcc ctgggccagc gctaccggaa agtgatcgcc tgggcgctcg accaccggct
     1201 cgcgacgatg ggcatcgcga ccgtggcgtt cttcgcggcg atgagcctgt tcgacctcgg
     1261 tctcgtgggc gggcagttca tgcccacctc cgacaacgag cagacgctgc tgacgatcga
     1321 gacgccggtg ggctcctcgg tggactacac cgcgggcaag gcgctcgagc tctcacacta
     1381 cctcgacagc ctgcccgagg tggcgttgac ctacgcgacc gtgggcggcg cccagcagaa
     1441 cgaatccgtc aacaaggggc agatctacgt caagctgacg ggggcgcact ctcgagcgcg
     1501 gtcgcagcag cagctcgaag cggacctgcg ccgggtgctg ccgcgcttcc agggagtggc
     1561 ggcgcgagtg tcgcaaatcg gcgcggcggg agggagccag gcgccgatcc agctgaacct
     1621 ccagggaccc gcgctggttc ggctccaggg actgtccgac agcgccctgg cggcgattcg
     1681 caccgtcccc ggactcgtgg acctgcgctc cagtctcgaa ggccgcaagc ctgagttcag
     1741 cgtggacgtg agccgcgacc tggccgccga cgtcgggctg tcggtcggcg gcatcggcgc
     1801 agcgctgcgg ccggtgctga ccggcgagca ggcgggcacc tgggaggatg agacgggcct
     1861 ggcgcacgac gtggtggtcc gcctcgtccc ggagtcgcgc cagacggagt ccgacctggc
     1921 ccggatcccg ctcccgtcga gccggatcga tgcccgcacc ggggcgccgg tcatgatccc
     1981 gctcgggcag gtggccacga tccgctcgag cggcgcgccc aacgagatca agcggctcga
     2041 cctcgagcgc gtggccacga tcgaggggaa ctatcagggc cggccgctga ccgacgtggt
     2101 caaggacgtg cgggcgcggc tcgccacgct cgacctgccg ccggggtacc gcttcgattt
     2161 cggcggcgag cagaaggact tcgccgagac cgtgggctac atgctcgagt cgctcaccct
     2221 ggcgatcgtc ttcatctatc tgatcctggc gtcgcagttc ggcagcttcc tgcagccgct
     2281 cgcgatcatg ctgtcgctgc cgctgtcgct cgtgggcgtg atgctgggct tgatgctcac
     2341 gcgcggcacg ttcaacatca tgagcatgat cggcgtgatc atgctgatgg gactggtgac
     2401 gaagaacgcg atcctgctga tcgatttcgc caacaaggcg agggagtcgg ggagcgaccg
     2461 gcgcgaggcc ctgatcgatg cgggcgagct gcggctgcgc cccatcgtga tgacgacgct
     2521 ggcgatgatc ttcggcatgc tgccgacggc gctcgcactg ggcgcgggcg ccgagttccg
     2581 cgcgccgatg gcgcacgcgg tgatcggcgg gctgatcacc tcgacgctgc tcacgctggt
     2641 cgtggtgccg gtggtctatt cctacctcga cgactttggc gcctgggtgg gggcgaaggt
     2701 gaagcgctgg accagcgcgc ccgagccggc gccggtgccc gtggagcgga gcttcaccga
     2761 gccggcgctg caccagtaag ccctgtggtg gccaccccct ctcccaaccg ggaggggggc
     2821 ggcgtatcgt ggtcgaaggc cataccagga gcccatcatg ccggagcagc tcgtgggaac
     2881 ggtgtcgcac tacttcaagg gaccgagcgt cgccatcgtg cgcctgacca ccggtccgct
     2941 ggcggtgggt gaccggattc actttcacgg tcacaccacc gacttcaccg agcagatcac
     3001 ctcgatggag gtgaaccatc agaaggtcga gcgcgccaac gtgggggaag aggtcgccat
     3061 ccaggtgacc gaccgcgcgc gcgaacacga ccaggtgctg caggtgaccg actagccggg
     3121 caccgcgcat gcagcgggtg atcctcgccg tcgtcgtggg ctgcgcgtgt gtcggcgaat
     3181 cgggcgatac cggccgggga ccgccggccc ggagcgcgct cgcggtccag gacacgacgg
     3241 cggcgttcgc cggcacgctc gagccggttc gccgcccctc cagctccatg cgggtgcacg
     3301 ccgtcgtgct gcaggcggtg cgcgccggcc cggagccggg gttcgaccgt gtcgtcttcg
     3361 agttcgctgg ccccggcctc ccgggatatc aggtccaata cgcgaccggg ccgatccagg
     3421 cctgcggatc cggggccgtg gtcccgctcg ccggcatgga tcacctgctg gtgcgattcg
     3481 agcccgcgca ggcgcacgac acgctgggtc gcgtcacggc agccgaccgg atgctcgcgc
     3541 cccggctccc gcaggtgctc gacgtgaagc tggtgtgcga tttcgaggga caggtcgagt
     3601 gggcggtcgg cgtcgcggcg cgccggccgt ttcgcgtgcg cgaggattcg cagccgccac
     3661 gcgtgatcgt cgacgtgcgc caccgtgagt gaccggcgcg ccgccttgtg gatcgccggc
     3721 gtagccctgg cggcctgccg gcagcccgcc ccgacgccgc aaccctgtcc gcaggcgagc
     3781 ccggcgcgtc tcgcgcggac gctgaacgtc gactcactcc cgtcgcggta cgacagtcaa
     3841 ttcaccgcgg ccgctgccga gttccgcgtg ccacgcggca tgctcaaggg catcggctgg
     3901 gtcgagacac ggtggcagat ggtacgcggg gaagaggagt tcccgggccg gccgcccgcg
     3961 ttcggcgtca tggcgctgcg cggaacggcg ctcgagcggg gcgcggcgct cgccggggtg
     4021 agcgtgcagg tcgcgcgcac cgatccgctc gccaatatcc gggccgcagc cgcgctgctc
     4081 gccgccgccg cggactcggc cggcatcgac cgcaccaagg cggaggcgtg ggagccgatc
     4141 gtcggccgtt tcagcgggat tgccgcgccc tatgcccgag cggcgtacac gctggacgtg
     4201 gaccggaccc tcgcccctca ggaacgcctg aacccgcagg tcacctgtcc cccacctccg
     4261 ccaccccctc cgcctccacc acctccacca cctccacctc ctccagaacc caagccagtc
     4321 tggcgccctt cgcctaacta caacaccagg ccgcaggact cggccggcac cgtccacatg
     4381 gtgatcatcc acacctgcga gagcggctac accggctgct ggggctggct cgtgaacccg
     4441 gcgtcgcagg tcagcgcgca ctacgtggtg gacgaggacg gcacaaatcg gaagagcaca
     4501 cgtctgaact ccagtc
//
LOCUS       DBNGZW010000102         6100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1235919, whole genome shotgun sequence.
ACCESSION   DBNGZW010000102 DBNGZW010000000
VERSION     DBNGZW010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6100
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1235919"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="AC16JC_02510"
     CDS             complement(<1..727)
                     /locus_tag="AC16JC_02510"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR5163599.1"
                     /translation="MSFEYHEPTSLAEAVDLGARFGADASFLAGGTDLIIQMRRGKVT
                     PPHVISLHRVPGLDAIEVNGRVRLGALVTHRAIERCAAFRGPLRGLIEGAEVVGGHQV
                     RNVGTVGGNVVNASPAADVVPVLLALDATVTCVGIDGERTEALEEFLVGPGRTRRRPG
                     ELLAALAFAKPEGRAATAFLKAGRRRAMEISVVCVAARLELDSADGRCVAARIALGAV
                     APATWRAHAAERVLVGSVPPPEVL"
     gene            complement(724..1581)
                     /locus_tag="AC16JC_02515"
     CDS             complement(724..1581)
                     /locus_tag="AC16JC_02515"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5163600.1"
                     /translation="MTAVTSRVSGTAVASVTRAMRVLEAFADHPGGVALTPLSAELGY
                     GKASLSKIMATMEREGFVRRDGAGHFHLSWRLLALAFGHAQRVGVSGVCLPVLQALAD
                     ETDELVQLAVIERDHVLFVAKAEGPGHALRLLPLVGVVAPTHSTASGKVWLASLPDGE
                     ALQVMRRKGLARVTSRTITSRGRLLAELRRVRRAGYAITDGELAEEGRAIAAPIVYGG
                     RVVGAIAVSGPSFRLPLPRLQRLAPRVQRAAHELQALWPYEVTARDFGLGVRPPNGNG
                     QVRPVRRHA"
     gene            complement(1973..2563)
                     /locus_tag="AC16JC_02520"
     CDS             complement(1973..2563)
                     /locus_tag="AC16JC_02520"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5163601.1"
                     /translation="MTKDAFAQGVVVAALALLWASPGMAQTPGSPGPVPPAAQSRFPA
                     TSFLAPTKATWDSTRTLVLGIADSVPEDKYDFKPTPAVRTFREILVHLIGENYTFFSR
                     VSGENLGNNTRFDTLKSRAEILKALRESYDYGAGVWAGLTEEKALEMVAGRGGQPTQR
                     WAAILGVIQDNMNHYGNLVVYLRLNGIVPPRSASRN"
     gene            2729..5284
                     /locus_tag="AC16JC_02525"
     CDS             2729..5284
                     /locus_tag="AC16JC_02525"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HIR5163602.1"
                     /translation="MTSPPVLRETLWNVPFWAALGVAILAGSSDIGHAAEPPTGAAIL
                     QQLRSFATMGSVLYIAAHPDDENTQILTYLARGRGYRTAYLSLTRGDGGQNLLGPQLG
                     EALGVARTQELLAARRLDGGRQYFTRAKDFGYSKNAEETLNVWDRPAVLGDIVRVIRE
                     FRPDVIITRFSPKPAPTHGHHTASAVLAVEAFRLAADPAAFPEQLRELRPWQARRIVH
                     NVGQGVGAANPGGGMAIAGSPANVPDGPGVVKVEVGGRDPVSGESFAAIAARSRGMHR
                     TQGFGIGAPPVHEGSRIEPFVLLAGDAATTDLLDGVDTTWNRLPGGAEIARAIDEAIA
                     TFNAQDPTAAVPALLAIRRRLAALPADPVVSDKRAQLDRIILAGLGLEVDTVADRAEV
                     VPGERVKLRHTAVVRSRIAVRWTAVRYPSVHRAITKAIALPPNQLVVRDASQVVPAST
                     PPSQPYWLRTEGTVGLFDVDDPSLIGRPENPPAFPLEYVFEVDGQTLVIAGEPRPAAD
                     PAAPALRRRLEVIAPVFVRFIAGVQLFAPGAARPVTVDLTAARARAVGTVQLDAPAGW
                     VVTPASQPFRLAAPGAHARFTFTVTAPPQLATGPLGASVEINGRRFDQQRIEVRYDHL
                     PLQLLQPPARARAVSLELATRGRHVGYLPGAGDDVPAALEQMGYEVTSLTGADLTPER
                     LRGLDAVVIGIRAFNVRTDLAERLPGLFAYVEAGGTVVAQYNTLDGLREGWLAPFQLH
                     LSRDRVTDEHAPVTILAPEHPVLTAPNRITAADFEGWVQERGLYFPDRWDDRFTAILA
                     SGDTGEPPLKGGLLVARHGNGYVVYTSLAWFRQLPDAVPGAYRLFANLVSLGK"
     gene            5281..5433
                     /locus_tag="AC16JC_02530"
     CDS             5281..5433
                     /locus_tag="AC16JC_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163603.1"
                     /translation="MTPAPRPDEPSETEPPDVPGFRTWRGIYLFVFGWFVLVVILLAM
                     FTGIFA"
     gene            5430..>6100
                     /locus_tag="AC16JC_02535"
     CDS             5430..>6100
                     /locus_tag="AC16JC_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR5163604.1"
                     /translation="MNVADWVVLLGTIVGIAAYGAWRTRHVRSLNTYLRGNASTGWGT
                     IGLSVMATQASAITFLSLPGQGFESGIGFVQNYFGLPLALIVVCAVFLPMYRRLNVYT
                     AYEFLGQRFDEKTRLLGAGLFLLQRGLAAGVTIYAPAIIISTVLGWPLNLVIVFTGLL
                     VIVYTVTGGSEAVALTHKWQMAVISAGMVTAMVVLLLRLPDGLGFSGAAHVAGALGKL
                     QAVDFS"
BASE COUNT          842 a         2213 c         2137 g          908 t
ORIGIN      
        1 ggagcacctc cggcgggggc acgctcccca cgaggacccg ttcggccgcg tgcgcgcgcc
       61 acgtggccgg tgccaccgct cccagcgcga tgcgcgcggc cacacagcgc ccgtccgcgc
      121 tgtcgagctc gagccgcgcg gcgacgcaca cgaccgagat ttccatcgct cgacggcgcc
      181 ccgccttgag gaaagcggtc gcggcgcgcc cttccggctt ggcgaaggcc agggccgcga
      241 ggagctcgcc gggccggcgc ctggtgcgcc ccggccccac caggaactcc tcaagggctt
      301 ccgtgcgctc gccgtcgatc ccgacgcacg tgacggtggc atcgagggcg agaagcacgg
      361 gtacgacgtc ggcggcgggc gacgcgttga cgacgttgcc gccgaccgtt cccacgttcc
      421 gcacctggtg cccccccacg acctctgcgc cctcgatgag gccgcgcagt ggcccccgga
      481 acgccgcaca gcgctcgatg gcccggtggg tcaccagcgc gccaaggcgc acgcggccgt
      541 tgacttcaat cgcgtccagg ccgggcacgc gatgcaggct gatcacgtga ggcggggtca
      601 ccttgccgcg ccgcatctgg atgatgaggt cggtgccccc ggcgaggaag gacgcgtcgg
      661 cgccgaagcg ggccccgagg tcgaccgcct cggcgagcga ggtcggctca tggtactcga
      721 agctcacgcg tgcctccgca ccgggcggac ttgcccgttg ccgttgggag gccggacccc
      781 cagtccgaag tctcgggccg tcacctcata cggccagagc gcctgcagct cgtgagccgc
      841 ccgctggacc cgcggggcaa gccgttggag ccggggaagc gggagccgga acgacggacc
      901 ggagaccgcg atggcgccaa cgacccggcc tccgtagacg atcggggccg cgatggctcg
      961 cccctcctcc gccagctctc catcggtgat ggcgtagccg gcgcggcgca cccgacgcag
     1021 ctccgcgagg agacgcccgc gcgaggtgat cgtgcgcgac gtcacgcggg cgaggccctt
     1081 gcgacgcatc acctgcagcg cctcgccgtc gggcaggctc gcgagccaga ccttgccgct
     1141 cgccgtcgaa tgcgtcggcg cgaccacgcc caccagcggg aggaggcgta gggcgtggcc
     1201 cgggccctct gccttcgcca cgaagagcac gtggtcgcgt tcgatcacgg cgagctgaac
     1261 cagctcgtcg gtctcgtcgg cgagggcctg gaggacgggc aggcagacgc cggagacgcc
     1321 aacccgctgg gcgtggccga aggcgagtgc gaggaggcgc cagctgaggt ggaagtggcc
     1381 ggccccgtcc cggcggacaa acccctcgcg ctccatggtg gccatgatct tggacaggct
     1441 cgccttgccg tagccgagct ccgcgctcaa cggggtgagg gcaacgccgc cgggatggtc
     1501 cgcaaacgct tcgaggacgc gcatggcgcg cgtgacagac gcgaccgcgg taccggagac
     1561 ccgagaggtg acggcagtca tttaggaaac tgagtttcct ctctataaca cccggcgaat
     1621 cctgtcaagc gccgcaggac cggtcgaggc cgcctcgtga ccaatcgtga cgttcgccta
     1681 ggcgctcggt tcgcagatca gaagaattgg gggccgctcg cttaccgggc tggtcggtca
     1741 ggccggggga acaagccggg gctactgaac ttgacgggat gtcgacacca tcgtctcatg
     1801 tcgcctcaag acgtcggctc agcatatgcc ggaccgccgc gctggacgcc cgcgcgggga
     1861 cgagacactg ccggcgatgg acggcacggg gcgggtcggg tggtctagtg acggggatgc
     1921 cgctcgccca tggcctttgg cccaccatgg cggtgactcc acgcgacgcc cgtcaattgc
     1981 gcgaagcgct gcgcggcggc acgatcccgt tgagtcggag gtacacgacc aggttgccgt
     2041 agtggttcat gttgtcctgg atcactccca gaatggccgc ccagcgctgc gttggctggc
     2101 cgcccctccc ggccaccatt tccagagcct tttcttcggt gagcccagcc cagaccccgg
     2161 ctccatagtc gtagctttcc cggagcgcct tcaggatctc cgcgcggctc ttgagggtgt
     2221 cgaagcgggt gttattgcca aggttctcgc cggagacccg gctgaaaaac gtgtagttct
     2281 ccccgatcag atggacgagg atctcccgga acgttcgcac ggcgggagtc ggcttgaagt
     2341 cgtacttgtc ttcgggcacg ctgtcggcga ttccgagaac cagagtgcga gtgctgtccc
     2401 acgtggcctt ggtcggcgcc aagaagctcg tggccgggaa tcgggactgg gcggccggag
     2461 gcacgggccc cgggctgcct ggcgtctgcg ccatccccgg gctggcccac agcagcgcaa
     2521 gcgcggcaac aacgacaccc tgtgcgaacg cgtccttggt catgatgcct cctgttcggt
     2581 gacgaaacgc cggcggcacg agctgctccc gcgcgtgacg cgtggatccg gctgaacgta
     2641 gataacataa atgcgaccag gagtctccct cgatacggcg gccgccgcgc gccttccgtt
     2701 ggatgccgtg ctatcgtgtg agccgccgat gacgtctccc cctgtactgc gcgagaccct
     2761 ctggaacgtg ccgttctggg ccgccctcgg cgtggccatc ctggcgggct cctccgacat
     2821 cggccacgcc gccgagccac cgaccggggc cgcgatcctg cagcagctgc gcagcttcgc
     2881 gacgatgggc agcgtgctct acattgccgc ccatccagac gacgagaaca cgcaaatcct
     2941 cacctatctg gcgcgcggcc gcggataccg gacggcctat ctctcgctca cccgcggcga
     3001 cggcggacag aacctgctcg gcccccagct cggcgaggcg ctcggcgtgg cgcgcacgca
     3061 ggagctgctc gccgcgcgcc ggctggacgg cgggcggcag tacttcacgc gcgcgaagga
     3121 cttcgggtac tcgaagaacg ccgaggagac gctgaacgtc tgggaccggc cggccgtcct
     3181 cggcgacatc gtgcgcgtga tccgtgagtt ccgcccggac gtgatcatca cgcggttctc
     3241 cccgaagccc gcgcccacgc acgggcatca caccgcgtcg gccgtgctcg ccgtcgaagc
     3301 gttccggctc gccgccgacc ccgcggcgtt tcccgagcag ctccgcgagc tccggccctg
     3361 gcaagcgcgc cgcatcgtcc acaacgtcgg ccagggcgtc ggtgcggcga atccgggcgg
     3421 cggcatggcc atcgccggct cgcccgcgaa cgtgccggat gggccgggcg tggtgaaggt
     3481 cgaggtcggc ggccgggatc cggtatcggg cgagtcgttc gcggcgatcg cggcccgcag
     3541 ccgtggcatg cacaggacgc agggtttcgg catcggcgct ccgcccgtgc atgagggctc
     3601 gcggatcgag ccgttcgtgc tgctcgccgg cgacgcggcg acgaccgatc tcctcgacgg
     3661 cgtggacacg acgtggaacc gcctgccggg cggcgccgag atcgcgcgcg cgatcgacga
     3721 ggcgatcgcg acgttcaacg cccaggaccc gaccgcggcc gtgcccgcgc tgctcgccat
     3781 ccgccgccgg cttgccgcgc tccccgcgga tcccgtcgtg agcgacaagc gggcgcagct
     3841 tgatcggatc atcctggcgg gcctcggcct cgaggtcgac accgtggcgg atcgcgccga
     3901 ggtcgtgccg ggtgagcgcg taaagctgcg ccacacggcg gtcgtgcggt cgcgcatcgc
     3961 cgtgcgctgg acggcggtgc ggtacccgag tgtgcaccgc gcgatcacca aggcgatcgc
     4021 gctgcccccg aaccagctgg tcgtccgcga cgcctcccag gtcgtgcccg cgagcacgcc
     4081 gccgagccag ccgtactggc tgcgcacgga gggcacggtg gggctgttcg acgtggacga
     4141 tccgtcgctc atcggccgtc ccgagaaccc gcccgcgttc ccgctcgagt acgtgttcga
     4201 ggtcgacggg cagacgctgg tgatcgccgg cgagccgagg ccggccgcgg atccggccgc
     4261 gcccgcgctg cgtcggcgcc tggaggtcat cgcgccggtc ttcgtgcggt tcatcgcggg
     4321 cgtccagctg ttcgcgcccg gcgccgcgcg tccagtgacg gtggacctca ccgccgcccg
     4381 cgcgcgcgcc gtcggcaccg tgcagctcga cgcgccggcc ggctgggtcg tgacgccggc
     4441 ctcgcagccg tttcgcctcg ccgccccggg cgcgcacgcg cgcttcacct tcaccgtgac
     4501 cgcgccgccg cagctcgcga ccgggccgct cggggcgagc gtggagatca acggccggcg
     4561 cttcgaccag cagcgcatcg aggtgcgcta cgatcacctg ccgctgcagc tcctgcagcc
     4621 cccggcgcgc gcccgggcgg tgtcgctcga gctggcgact cgcggccgcc acgtcggcta
     4681 cctgccgggc gccggcgacg acgtgcccgc cgccctggag cagatgggct acgaggtcac
     4741 gtccctgacc ggcgccgatc tgacgccgga acggctgcgc ggcctcgacg cggtggtcat
     4801 cggcatccgc gccttcaacg tgaggacgga tctcgccgag cgcctgcccg ggctcttcgc
     4861 gtacgtcgag gccggcggca cggtcgtcgc gcagtacaac accctggacg gtctgcgcga
     4921 ggggtggctc gccccgttcc agctgcacct ctcgcgcgac cgtgtgaccg acgagcacgc
     4981 gccggtgacg atcctggcgc ccgagcatcc ggtgctgacc gcgccgaatc gcatcaccgc
     5041 ggcggacttc gagggctggg tacaggagcg cggtctctac ttccccgacc gctgggacga
     5101 ccgcttcacc gccatcctcg cctccggcga caccggcgag ccgccgctca agggcggcct
     5161 gctggtggcg cggcacggca acggctacgt cgtctacacc agtctggcgt ggtttcgcca
     5221 gctccccgac gccgtgcccg gcgcgtatcg gctgttcgcg aatctcgtct cgctgggcaa
     5281 atgacgcccg cgccgcgccc cgacgagccc tcggagaccg agccgcccga cgtccccggg
     5341 ttccgcacct ggcgcgggat ctacctgttc gtgttcgggt ggttcgtact cgtggtgatt
     5401 ctgttggcca tgttcacggg gatcttcgcg tgaacgtcgc cgactgggtc gtgctgctcg
     5461 gcaccatcgt gggcatcgcc gcgtacgggg cctggcgcac gcgccacgtg cggagcctca
     5521 acacctacct gcgcggcaac gcgtccaccg gctggggtac gatcggcctc agcgtgatgg
     5581 ccacgcaggc gagcgcgatc acgttcctct cgctcccggg ccagggcttc gagagcggca
     5641 tcggcttcgt gcagaactac ttcggcttgc cgctggcgct catcgtcgtg tgcgccgtgt
     5701 tcctgcccat gtaccggcgg ctcaacgtct acaccgccta cgagttcctc ggccagcggt
     5761 tcgacgagaa gacgcgcctg ctcggcgccg gcctcttcct gctgcagcgt gggctcgccg
     5821 ccggcgtgac gatctacgcg cccgcgatca tcatctcgac cgtcctcggc tggccgctca
     5881 acctggtcat cgtcttcacc gggctgctcg tgatcgtcta cacggtgacc ggcggcagcg
     5941 aagccgtcgc cctgacccac aagtggcaga tggcggtgat ctcggccggc atggtgacgg
     6001 ccatggtcgt gctgctgctc cggctgcccg acggcctcgg gttctccggc gccgcgcacg
     6061 tggccggcgc gctgggcaag ctgcaggcgg tggacttctc
//
LOCUS       DBNGZW010000103         3071 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1509091, whole genome shotgun sequence.
ACCESSION   DBNGZW010000103 DBNGZW010000000
VERSION     DBNGZW010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1509091"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1036)
                     /locus_tag="AC16JC_02540"
     CDS             complement(56..1036)
                     /locus_tag="AC16JC_02540"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HIR5163605.1"
                     /translation="MPTITYRDALNQALREEMQRDPAVFLMGEEVGLYQGAYKVSRGL
                     LEEFGAQRVVDTPIAELGFAGIGVGAAMGGLRPVIEFMTWNFALLALDQIVNSAAKML
                     YMSGGQLPMPIVFRGPNGAALQLASQHSQAWESWLAHIPGLKVVAPATPADAKGLLKS
                     AIRDNNPVVVLEGEMLYNTKGEVPEGELLVPIGRADVKRAGSDVTVICHSKTVAPALK
                     AAEQLAGQGVAAEVVDLRSLRPLDESAVLASVAKTHRAVIAEEGWPHSGIGAQVVDII
                     QRDAFDELDAPVLRVTQADAPMPYNKQLERLAKPTPDKIVAAARRVLYLD"
     gene            complement(1041..2042)
                     /gene="pdhA"
                     /locus_tag="AC16JC_02545"
     CDS             complement(1041..2042)
                     /gene="pdhA"
                     /locus_tag="AC16JC_02545"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIR5163606.1"
                     /translation="MTTATAQPQHQSPTDAALQRRMLREMLLIRRFEEKAAEAYALGK
                     IGGFCHLYIGQEAVAVGVSAALTPSDYVISSYREHGQALVRGISARAVMAELFGKATG
                     CSGGKGGSMHLFDAEKRFMGGHGIVGGHLPLATGLAFAIKYRGGDEVCVCFFGEAAVN
                     IGAFHEAVNMAAVWKLPVIFLGENNRYGMGTAFERVAAVTDVVEHACSYDIAGEVVNG
                     MDVLEVHQAAGRAIQRARKGGHPTLLEVRTYRFMGHSMSDPLHGVYRTKDEVEEQKRR
                     DPITQLSHQLLQAGAIDQAGIDALDAEVHAEVEDAITFADASPEPAPEELLRHVIVD"
     gene            complement(2039..2944)
                     /gene="lipA"
                     /locus_tag="AC16JC_02550"
     CDS             complement(2039..2944)
                     /gene="lipA"
                     /locus_tag="AC16JC_02550"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR5163607.1"
                     /translation="MAAPLIQIHPRSAIPAARKPPWLKVKAPGGTTYLSVQRTMRDLR
                     LHTVCEEARCPNIGECWEHRAATFMIMGDVCTRNCAYCAVAHGTPAPLDPEEPARLAA
                     AVEQMGLRHVVITSVDRDDLEHGGAEIFAACVTEIRRRLPDTTVEVLIPDFKGSEAAL
                     RLVLDAAPAILNHNLETIERLYRIARPGGRYARALELLRRARTIRSDGLTKSGLMCGL
                     GEEWDELLVAMRDLRAQDVDILTLGQYLRPSESHLPIARWYTPDEFAELRRIGLAMGF
                     RHVESGPLVRSSYHAWEQVAAVTTP"
BASE COUNT          466 a         1057 c         1061 g          487 t
ORIGIN      
        1 gcggccctcc tccatcgtgg gcgagagcgc ctccatcacg accttggcgg ccacgtcagt
       61 cgaggtagag gacccggcgg gcggccgcga cgatcttgtc gggcgtcggc ttggccaggc
      121 gctcgagctg cttgttgtag ggcatcggcg cgtccgcctg cgtgacccgc agcaccgggg
      181 catcgagctc gtcgaacgcg tcgcgctgga tgatgtccac cacctgcgcg ccgatgccgc
      241 tgtggggcca cccttcctcg gcgatgacgg cccggtgcgt cttcgcgacg gaggcgagga
      301 cggccgactc gtccagcggc cgcagcgagc ggagatccac cacctcggcg gcgacgccct
      361 ggcccgcgag ctgctccgcc gccttcaacg ccggggcgac ggtcttggag tggcagatca
      421 ccgtgacatc gctgccggcc cgcttcacgt ccgcccggcc gatcggcacc agcagctcgc
      481 cctcgggcac ctcgcccttg gtgttgtaga gcatctcgcc ctccagcacc accacggggt
      541 tgttgtcgcg gatcgcggac ttgagcaggc ccttcgcgtc cgcgggcgtc gcgggggcga
      601 ccaccttcaa ccccgggatg tgcgccagcc acgattccca ggcctgcgag tgctgggagg
      661 cgagctgcag cgcagcgccg ttgggaccgc gaaacacgat cggcatgggg agctggccgc
      721 ccgacatgta gagcatcttc gccgcggaat tcacgatctg gtccagggcc agcagcgcga
      781 agttccacgt catgaactcg atgacggggc gcaggccgcc catcgcggca ccgaccccga
      841 tgccggcgaa gccgagctcg gcgatcggcg tgtccaccac gcgctgcgcg ccgaactcct
      901 cgagcagacc ccgactcacc ttgtaggcac cctggtagag tccgacttcc tcgcccatga
      961 ggaagacggc gggatcacgc tgcatctcct cccgcagcgc ctggttcagg gcgtcccggt
     1021 acgtgatggt cggcatcggg tcagtccacg atcacgtggc gcagcagctc ttcgggggcc
     1081 ggctccgggc tcgcgtcggc gaacgtgatc gcatcctcga cctcggcgtg cacctcggcg
     1141 tcgagcgcgt cgatgccggc ctggtcgatc gccccggcct gcaacagctg gtgggagagc
     1201 tgcgtgatgg gatccctgcg cttctgctct tccacctcgt ccttggtgcg gtacacgccg
     1261 tgcagcgggt cggacatcga gtgacccatg aagcggtagg tgcggacttc gagcagggtg
     1321 gggtggccgc ctttgcgggc acgctgtatg gcgcgcccgg ccgcctgatg gacctccagc
     1381 acgtccatgc cgttcacgac ttcgccggcg atgtcgtaac tgcaggcatg ctccacgacg
     1441 tcggtcacgg cggccacgcg ctcgaaggcg gtccccatgc cgtagcggtt gttctcgccc
     1501 aggaagatca ccggcagctt ccacaccgcg gccatgttga ccgcctcatg gaaggcgccg
     1561 atgttcacgg ccgcctcgcc gaagaagcag acgcacacct cgtcgccgcc gcggtacttg
     1621 atggcgaagg cgagccccgt ggcgagcggc aggtgaccgc ccacgatgcc gtgccctccc
     1681 atgaagcgct tctccgcgtc gaacaggtgc atcgagccgc ccttgccgcc cgagcacccg
     1741 gtcgccttgc cgaacaactc ggccatcacg gcacgggcgg agatgccgcg gaccaacgcc
     1801 tggccgtgct cgcggtagga ggagatgacg tagtcggacg gggtgagcgc ggccgacacg
     1861 ccgaccgcga ccgcctcctg gccgatgtag aggtggcaga agccgccgat cttgccgagc
     1921 gcgtaggctt cggcggcttt ctcctcgaag cggcggatga gcagcatctc gcgcagcatg
     1981 cggcgctgca gcgccgcatc cgtcgggctc tggtgctgcg gctgggcggt ggcggtagtc
     2041 atggcgtcgt caccgccgcg acctgctccc aggcgtgata gctcgagcgc accaggggac
     2101 ccgactcgac gtgacggaac cccatggcga gaccgatccg ccggagctcc gcgaactcgt
     2161 ccggggtgta ccagcgcgcg atcggcagat ggctctcgga ggggcgcaag tactggccca
     2221 gcgtgagaat gtccacgtcc tgcgcgcgca ggtcccgcat cgccacgagc agctcgtccc
     2281 attcctcccc cagtccgcac atcaggcccg acttggtgag gccgtcggac cggatggtcc
     2341 tggcgcgccg caacagctcg agggcccgcg catagcgtcc gcccggacgg gcgatgcggt
     2401 acagccgctc gatcgtctcg agattgtgat tcaggatggc gggcgcggcg tccaggacga
     2461 gccgcagcgc cgcttcgctg cccttgaagt cgggaatgag cacctcgacg gtcgtatccg
     2521 gcaggcggcg gcggatctcg gtgacgcagg ccgcgaagat ctcggcgccg ccgtgctcga
     2581 ggtcgtcgcg gtccacactg gtgatgacga cgtgtcgcaa tcccatttgc tcgaccgcgg
     2641 cggccaaccg tgcgggctcc tccggatcga gcggcgccgg ggtgccgtgc gccacggcgc
     2701 aataggcgca gttgcgggtg cagacgtcgc ccatgatcat gaaggtggcc gcccggtgct
     2761 cccagcactc gccgatattg gggcagcgcg cttcttcaca cacggtgtgc agccgcaggt
     2821 cgcgcatcgt tcgctggacc gacaggtacg tcgtgccgcc gggagctttt accttgagcc
     2881 acggcggctt gcgagccgcg gggatggcgg agcgcggatg gatctgaatc agcggtgcag
     2941 ccatacgact ccggcgaccg ggagaaacat gaagatagtg agggcgggga gcctggggta
     3001 gacgaaattc aaccggatgg ttgattccat cgccgcttga gccgactgag cttctcttcg
     3061 atcgccgagt c
//
LOCUS       DBNGZW010000104         1960 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_622281, whole genome shotgun sequence.
ACCESSION   DBNGZW010000104 DBNGZW010000000
VERSION     DBNGZW010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_622281"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1340)
                     /gene="uvrC"
                     /locus_tag="AC16JC_02555"
     CDS             complement(<1..1340)
                     /gene="uvrC"
                     /locus_tag="AC16JC_02555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HIR5163608.1"
                     /translation="MDIPALIARKLAALPDQPGVYLWRNAAGEVLYVGKAKSLRNRVP
                     AYFAPDHQDTPERAALATQIADLETIIVPDEAQALLLENNLIKEHQPRFNIRLRDDKS
                     YPRIAVTVGEPFPRVLVVRRVQLEGARYFGPYTDVRTLRQTLGIIRRLFTVRSCQYDL
                     PRDAPNRPCLDYHIERCRAPCVGYQSEAEYRRMIDDVLLFLEGKTVEVRTQLRERMRQ
                     AAAEQDFERAAQLRDALQWLEQVERPQSVEQVGGEDADAIGLARDGDDACGVFLRIRG
                     GRLIAREHRFLERAGAEPEAGVLAAFLVRFYLPLEARARRVLLPFVPDDLEGLRALAP
                     GVEWLVPQRGTNAKLVELADQNARHLLESLLLESFDVEERAADPVYALGRDLGLASVP
                     RAFVCIDISTNQGRDTVGSLVWFEAGRPKKAEYRRYRIRGAQQDDFAAVHEVVTR"
     gene            complement(1322..>1960)
                     /locus_tag="AC16JC_02560"
     CDS             complement(1322..>1960)
                     /locus_tag="AC16JC_02560"
                     /inference="COORDINATES: protein motif:HMM:NF015013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HIR5163609.1"
                     /translation="IGAGPNDQLRARIAAGFRRITYFVVPASFLFAALGPVLVAALFQ
                     TGRFDAEDTALVGGVLAAYGIGLMGQATVKLFASGFYALRDTRTPVKIAAFSVMLSSA
                     LAYLLMRRFGPAGIALGSSLGAWVNVILHLRDLDRRIGTVMSGREWRSFATTLLSALA
                     ATAAGVAVAHLAAGLSPIPLAVVVVGIFGVGYFVCTLVLRHPDAARLWTSLR"
BASE COUNT          310 a          719 c          659 g          272 t
ORIGIN      
        1 cgggtcacga cctcgtggac ggccgcgaag tcgtcctgtt gcgctccccg gatgcgatag
       61 cgccggtact cggccttctt gggacgaccc gcctcgaacc acaccagcga gccaaccgtg
      121 tcgcgtccct ggttggtgga gatgtcgatg cacacgaacg cgcgcggcac gctggccagc
      181 ccaagatccc gccccagggc gtacaccggg tcggccgccc gctcttcgac atcgaacgat
      241 tcgagcagca ggctctccag cagatggcgc gcgttctggt cggccagctc gaccagcttc
      301 gcattggtgc cccgctgcgg cacgagccac tcgaccccgg gcgccagagc ccggagtccc
      361 tccagatcgt cggggacgaa cggcagcaac acccgacgcg cccgcgcctc gagcggcagg
      421 tagaaccgga cgaggaaggc cgccaggacg cccgcctccg gctcggcgcc ggcgcgctcc
      481 aggaaccggt gctcccgggc gatcaggcgg ccgccgcgga tccgcaggaa cacgccgcag
      541 gcatcgtccc cgtcgcgcgc cagcccgatt gcgtccgcgt cctcgccgcc gacctgctcc
      601 acgctctgcg gccgctcgac ctgctccagc cactgcagcg cgtcccgcag ctgcgccgcc
      661 cgctcgaagt cctgctccgc cgccgcctgc cgcatgcgct cccgcagctg ggtgcggact
      721 tcgaccgtct ttccctccag gaacaggagc acgtcgtcga tcatccgccg gtactcggct
      781 tcgctctggt agccgacgca cggcgcccgg cagcgctcga tgtggtagtc gagacagggc
      841 cggttgggcg cgtcgcgcgg caggtcgtac tgacagctcc gcacggtgaa caaccgtcgg
      901 atgatcccca gtgtctggcg caacgtccgc acgtcggtat acggtccgaa gtaccgcgcc
      961 ccctccagct ggacccgtcg caccaccagc acgcggggga acggctcgcc gaccgtgacc
     1021 gcgatgcgcg ggtaactctt gtcgtcccgc agccggatgt tgaagcgcgg ctggtgttcc
     1081 ttgatcaagt tgttctcgag cagcagcgcc tgcgcctcgt cggggacgat gatggtctcg
     1141 agatcggcga tctgcgtcgc gagcgcggcc cgctccgggg tgtcctggtg gtccggtgcg
     1201 aagtaggcgg ggacccggtt ccgcaggctc ttcgccttgc cgacgtaaag cacctcgccg
     1261 gcggcgtttc tccagagata cacgccgggc tggtccggca gcgcggcgag tttgcgggcg
     1321 atcagcgcag ggatgtccat aggcgggcag cgtcggggtg ccgcagtacc agggtgcaca
     1381 cgaagtatcc gacgccaaag atacctacca ccaccaccgc cagcgggatt ggggagagcc
     1441 ccgccgccag gtgcgctacg gccacccctg cggcggtcgc ggcgagggcc gacagcagcg
     1501 tcgtggcgaa ggatcgccac tcccgtccgc tcatcaccgt gccgatgcgg cgatcgaggt
     1561 cgcgcagatg caggattacg ttcacccagg cccccaggga cgagcccagc gcgattcccg
     1621 ccggcccgaa gcggcgcatg agcaggtagg cgagggcgct cgagagcatc accgagaagg
     1681 cggcgatctt caccggcgtg cgcgtatcgc gcagggcgta gaatcccgag gcgaacagct
     1741 tcaccgtcgc ctgccccatc aggccgatcc cgtacgccgc cagcacgccg cccacgagcg
     1801 ccgtgtcctc cgcatcgaac cgcccggtct ggaacagggc ggcgaccagc accggtccga
     1861 gcgcggcgaa caggaacgac gccggcacca cgaagtaggt gatgcgccgg aaccccgccg
     1921 cgatgcgggc acgcagctga tcgttgggac cggcgccgat
//
LOCUS       DBNGZW010000105         4185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_827510, whole genome shotgun sequence.
ACCESSION   DBNGZW010000105 DBNGZW010000000
VERSION     DBNGZW010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_827510"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..217)
                     /locus_tag="AC16JC_02565"
     CDS             complement(<1..217)
                     /locus_tag="AC16JC_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5163610.1"
                     /translation="MILVTGASEGLGRACARALLARTDSEVLITGRSVAKLHAAREAL
                     APSERERLRTLASDQSSRTDVDALVAFL"
     gene            complement(214..1206)
                     /locus_tag="AC16JC_02570"
     CDS             complement(214..1206)
                     /locus_tag="AC16JC_02570"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="HIR5163611.1"
                     /translation="MRARIAGAGHYLPGPPVTNEELIARHGLRVKSYFIRRSVGAETR
                     HFAADAQATSDLAVEAAVRAIEASGISKGDLRRIILATVTGDYPTPATACIVQRKLGL
                     KGLASIDVVGACAGFLQAFDLGARCVVTGEGPVLVIGADIRSRQLNYEDLRTVFLYGD
                     GAGAVVLTASEGDEGLHHSILTADGAGAEAVYVPAGGSREPVTEDALRCRRNTITMHN
                     GRLVAEAARDGFRQLASRLVAETHVPLADIDFFCLHQPNRFLVGQILEDLRVPEAKTW
                     INFPRYANTTSATVPIALSEAIAARRIPPGGWLCLGAVGAGFSGAIQLIRWGRS"
     gene            complement(1216..2565)
                     /locus_tag="AC16JC_02575"
     CDS             complement(1216..2565)
                     /locus_tag="AC16JC_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR5163612.1"
                     /translation="MSAGQHRFLAPMLASFGFERLQVWIDVGIPPERFSRSVCEGLVT
                     AVGHAEGRTVGLAWSDFRVNGAAFGRANSQRFAAFLRELDASPGESVPLVYLVNSAGV
                     SLMEGRQVFSPAFGLWPALLDYAERHPVLTAAVGKCLGLAPLLYGLGHYRVAVGGHTQ
                     INLTGPEVIALFFGQGIDFARDTAAERFHERTDLVHEVVPSLEAAFARFRSLLFPVAP
                     LHPPEALGPRSAALLASLLDAPPVELVPGWCRRVRVFLGTRGGRPLGLFLNPPERSDN
                     MITVRTLEKYAAGLDLFRALRVPIVSLVDSPGMDPRLDQSDANNFRKMLWVGEKVIRY
                     PYGAMGVVTGRCFGGATTLLFPKVFGGTTVIALAGSRIGAMHESIVERVLQHSPRLLE
                     QWHQVAARQDAQLADLVREGSLDAVVEPAALPGEIDRFLTYCALSRSGGATRLRHRA"
     gene            2896..3699
                     /locus_tag="AC16JC_02580"
     CDS             2896..3699
                     /locus_tag="AC16JC_02580"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011289634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR5163613.1"
                     /translation="MAAIILGLVEGATEFLPVSSTGHLILAGHALGFVGPRADAFEIV
                     IQLGAILAVVWLYRELLWRVVRGGVAAGESRRLVLALFVAFLPAAIVGLLTHHWITAH
                     LFTPAVVIAALIAGGVAILAIERWRPSPRIESVMQIGYTAALWIGIAQLCSLIPGVSR
                     SGATIMGALLVGVARPAAAEFSFLLAIPVMFAATGLELWENRDLLSGSDAAVFAIGFA
                     MAFASALVVVKWLVRFVSHRTFDVFAWYRIVFGLALLVLLATGRAWITK"
     gene            3696..>4185
                     /locus_tag="AC16JC_02585"
     CDS             3696..>4185
                     /locus_tag="AC16JC_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163614.1"
                     /translation="MRLLWLLLLLTACSRSAPPALAITHIAVVDVEHGSLLLDRTVVI
                     RGARIDRMDSAARISLPPGARTLDGSGRFLIPGLWDMHIHVDDPHTAAQLLGWGITGA
                     RMMGGPLEETLALRQRFRGDPLHGPRLLVVGLALHGPESFGTDTGMALVRTAAAGRRA
                     VDS"
BASE COUNT          621 a         1476 c         1459 g          629 t
ORIGIN      
        1 cgaggaaggc gacgagcgcg tccacgtcgg tgcggctgct ctggtcgctc gccagggtgc
       61 ggagacgctc ccgctcggac ggcgccaacg cctcccgcgc cgcatgcagc ttcgcgacgc
      121 tgcgcccggt gatcaggacc tcggaatccg tgcgggcgag caacgccctg gcgcatgccc
      181 ggcccagtcc ctcgctcgcc ccggtgacga ggatcatgac cgcccccagc ggatcagctg
      241 gatcgcgccg gagaatcccg cgccgaccgc acccagacag agccatccgc cgggcggaat
      301 ccggcgggcg gcgatggcct cggagagcgc gatcggcacc gtcgccgacg tcgtgttcgc
      361 gtagcggggg aagttgatcc acgtcttcgc ttcgggaacg cgcagatcct ccaggatctg
      421 tcccacgaga aagcggttcg gctgatgcag gcagaagaag tcgatgtcgg cgagcgggac
      481 gtgcgtctcg gcgaccaggc ggctcgcgag ctggcggaac ccgtcccggg ccgcctcggc
      541 taccaaccgt ccgttatgca tcgtgatcgt gttgcggcgg caacgcagcg cgtcctccgt
      601 caccggctcg cgcgagccgc cggccgggac atacaccgcc tccgccccgg cgccgtccgc
      661 cgtgaggatc gagtggtgga ggccctcgtc cccttcgctc gccgtcagga ccaccgcccc
      721 cgccccgtct ccatagagga agacggtgcg gagatcctcg tagttgagct ggcgcgagcg
      781 gatgtccgcc ccgatcacga ggaccggccc ctcgccggtc accacgcagc gtgcgccgag
      841 atcgaacgcc tgcagaaaac ccgcgcatgc ccccaccacg tcgatcgacg cgaggccctt
      901 gaggcccagc ttccgttgca cgatgcaggc ggtggcgggc gtcggataat cgccggtgac
      961 ggtggcgagg atgatccgcc gcaggtcgcc cttggaaatc ccgctggcct cgatcgcccg
     1021 caccgccgcc tcgacggcga ggtccgaggt ggcctgcgcg tcggcggcga agtgacgggt
     1081 ctcagccccg acgctgcgcc ggatgaagta ggatttcacc cgcaggccgt ggcgcgcgat
     1141 gagctcctcg ttggtgaccg gaggtcccgg gaggtagtgg ccggcgccgg cgatgcgggc
     1201 tctcatgcga ccctctcacg ctcggtgccg caggcgggtg gcgccgccgc tgcgggacag
     1261 ggcgcagtag gtcaggaagc gatcgatttc gccggggagc gccgccggct cgacgaccgc
     1321 gtcgagcgag ccttcgcgca ccagatccgc aagctgcgcg tcctggcgtg cggcgacctg
     1381 gtgccactgc tccagcagcc gcggactgtg ctgcagcacg cgctcgacga tgctctcgtg
     1441 catcgcgccg atccggctgc cggccagcgc gatcaccgtg gtgccgccga acaccttggg
     1501 aaacaggagc gtcgtggctc ccccgaagca gcgaccggtg acgacaccca tcgccccgta
     1561 ggggtagcga atcaccttct cgcccaccca cagcatcttc cggaagttgt tcgcgtcgct
     1621 ctggtcgagc cggggatcca tgcccggtga gtcgacgaga ctcacgatcg gcacccggag
     1681 cgcgcggaag agatcgagac cggcggcgta cttctcgagg gtgcgcaccg tgatcatgtt
     1741 gtcggagcgc tccggcgggt tgaggaacag gcccagcggg cgaccgcccc gcgtgccgag
     1801 aaacacgcgc acgcgccggc accaaccggg gaccagctcg accggcggcg catcgagcaa
     1861 cgacgccagc agcgccgcac tgcgcggccc cagcgcctcc ggcgggtgga gcggcgcgac
     1921 tggaaagagc aggctgcgga agcgcgcgaa cgccgcctcc agcgagggga cgacctcgtg
     1981 taccaggtcg gtccgttcgt ggaaccgctc ggcggcggtg tcccgggcga agtcgatccc
     2041 ctgcccgaag aagagcgcga tgacctcagg gccggtgagg ttgatctggg tgtggccgcc
     2101 gacggcgacg cggtaatggc ccaatccata cagcagcggg gcgaggccga ggcacttccc
     2161 cacggcggcc gtcagcaccg ggtgccgctc cgcataatcg agcaatgccg gccagaggcc
     2221 gaaggccggc gaaaagacct gccgcccctc catgaggctg acgccggccg agttcacgag
     2281 gtagacgagg ggaaccgact cccccggcga ggcatcgagc tcgcgcagaa aggccgcgaa
     2341 gcgctgcgag ttcgcccgcc cgaacgcggc accgttcacg cggaaatcgc tccaggcgag
     2401 ccccacggtg cgaccttcag catggcccac ggcggtcacc agcccttcac agaccgaccg
     2461 cgagaacctc tccgggggaa tgccgacgtc gatccacacc tgcaggcgct cgaatccgaa
     2521 cgacgccagc atgggggcga ggaaccggtg ttgcccggcc gacacggtga tccattggta
     2581 ctccggcggc gcgtggacgg cggacgtcct gcccgagtgc cggtgacttc cccgcacagc
     2641 caacggggaa aaccggaagc gctcccttct gccggcctgc tccatatgca ctccgcccca
     2701 gagttcactg ggacgatgcg ggggccgagt caagagactg tcaagtaagt cggacgcgac
     2761 gacggtcgcc gcacggttct gcaagacaat ttcagagagt ctgtggcgca ggtttagaaa
     2821 ggccgtgcag gtagacaggc cgcgagtgcc ggtgcaggtt tcctccgcca ccgaggagga
     2881 accctgcacg acatcatcgc cgcgatcatc ctcggcctcg tcgagggcgc gaccgagttt
     2941 ctccccgtgt cttccacggg gcatctgatc ctggcgggtc acgccctcgg cttcgtgggc
     3001 ccgcgcgccg acgcgttcga gatcgtcatc cagctcggcg ccatcctggc cgtcgtctgg
     3061 ctgtaccgcg agctgctgtg gcgcgtggtg cgcggcggcg tggccgccgg ggagtcacgc
     3121 cgtctggtgc tggccctgtt cgtcgcgttc ctgcccgcgg ccatcgtggg cctgctcacc
     3181 catcactgga tcaccgcgca tctgttcacg cccgcggtgg tcatcgcggc gttgatcgcg
     3241 ggcggcgtcg ccatcctcgc catcgagcgc tggcgcccct ctccgcggat cgagtcggtg
     3301 atgcagatcg gatacaccgc ggcgctgtgg atcgggatcg cgcagctgtg ctcgctcatc
     3361 cccggcgtgt cgcgctcggg agcgacgatc atgggggcgc tgctggtggg cgtcgcgcgg
     3421 ccggccgccg ccgagttctc ctttctgctc gccatcccgg tgatgttcgc ggccacgggc
     3481 ctcgagctgt gggagaaccg cgacctcctg tcggggtccg atgccgcggt cttcgcgata
     3541 gggttcgcca tggcgttcgc cagcgcgctg gtggtggtga aatggctcgt gcgcttcgtc
     3601 tcgcatcgta ccttcgacgt gttcgcctgg taccgcatcg tgttcgggct cgcgctgctg
     3661 gtgctgctcg ccacgggacg ggcgtggatc acgaagtgag gctcttgtgg ctcctcttgc
     3721 ttctcaccgc gtgcagccgg tcggcacctc cggcactcgc catcacgcac atcgccgtcg
     3781 tggacgtcga gcacggctcc ttgctgctcg accgcaccgt agtgatacgc ggcgctcgca
     3841 tcgaccggat ggacagcgcg gcgcggatca gcctgccgcc cggggcccgg acgctggacg
     3901 ggtccggtcg cttcctcatc cccgggctct gggacatgca catccacgtg gacgatccgc
     3961 acacggctgc gcagctcctg ggctggggca tcaccggcgc ccgcatgatg ggcggccccc
     4021 tggaggagac cctcgcgctg cggcagcgtt tccggggcga tccgctccac ggacctcgcc
     4081 tcctcgtggt cggcctggcg ctgcacgggc cggagtcgtt cgggaccgac accgggatgg
     4141 cgctggtcag gaccgcggcg gcgggacgcc gcgcagtcga ctcgc
//
LOCUS       DBNGZW010000106         4706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_895200, whole genome shotgun sequence.
ACCESSION   DBNGZW010000106 DBNGZW010000000
VERSION     DBNGZW010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4706
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_895200"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1476
                     /locus_tag="AC16JC_02590"
     CDS             <1..1476
                     /locus_tag="AC16JC_02590"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HIR5163615.1"
                     /translation="VQRGSVMDMPTYPCDPLSPGWAAVAGGRRLTQAEATTLEKIPVL
                     PIGYGDALPLLRTLTGPVAPESWRGSLPLTYHIGPGTTKVHLALSFEWRSRPLYDVIA
                     TIPGAVWPDQWVIYGNHHDAWVNGAEDPISGQAALGETARALATLLKTGWRPARTIVL
                     AAWDGEEWGLLGSTEWAEAHAGELRTKAVVYYNSDTNGAGWIGAAGSHALEQFITEVA
                     RDVTDPLTGKSALQASLDHRRAERTPRDTSRDTTFALGALGSGSDYTAFIDYLGLPSV
                     NLSYGGAAHSGIYHSIYDSYTFYERFLDTGYVYGVTEAQTMATAVLRMADAPLLPFEF
                     RAPTRTYRRYADEIARLAAKNDTTKGLDLAALRTALDRLDTAAGSWERERAAFDAVRG
                     RDAARRRAGLGAVNRLLASSEQALTDSAGLPRRPWFRHLIYAPGFYTGYGVKTMPGIR
                     EAVEQADATEAQAQAARVAAALTRYADVVHRAADALAAALR"
     gene            1553..2167
                     /locus_tag="AC16JC_02595"
     CDS             1553..2167
                     /locus_tag="AC16JC_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163616.1"
                     /translation="MSAPLPPRPSMPRSSFAIAMKDYTLIRPGIAITISAATLLAALI
                     IGVLLIRSLASASATVNRGATASEALHRLNAKYEVWRTMATGGDTTYRAQADSIRGSL
                     QTDLSTFSTSLANEQERSYVQQILEGLNNTDAPTTKNAREAMIVLLAHQDAALFAAAR
                     TSQQGVVFAAVLLALTILAAGMLIVPMAWLYVRYKRGATIEVKM"
     gene            2168..4039
                     /gene="nagB"
                     /locus_tag="AC16JC_02600"
     CDS             2168..4039
                     /gene="nagB"
                     /locus_tag="AC16JC_02600"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009579339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004342 - glucosamine-6-phosphate
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0006044 - N-acetylglucosamine metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HIR5163617.1"
                     /translation="MAGGLEGPRDRERLRTVVVADPDDLAALLADRIVAAIRRDTAAK
                     GRCVLGLATGSTPLGVYRELIRRHRAGEVEFSRVIAFNLDEYFPMSPDSPHSYRRYMW
                     ENFFAHVGMRPEQTHIPTGDTPRDGIAQACADYERSIVDAGGIDFQLLGIGQSGHVGF
                     NEPGSARESRTRLVMLDALTRKSAAADFFGEDNVPREAITMGVATILAAREIALVATG
                     EHKAVIVRRAVEGEPSPDVAATYLQGHPDATVYLDLAAAADLTRIRTPWVLGPVEWTP
                     ALTERAVVWLAERTDKAILKLTAADYTAHHLSPLLAQAGAAGPINGLVFNRLRDKIRG
                     RRKLPAGRRVLVFSPHPDDDVISMGGILRKLHENTNEITVAYMTSGNIAVFDHDVRRH
                     LDFVERAAAALEVDPAAVRRVSRAVLSQYERKTPGDVDLPVVQDLKRFIRESEAIAAL
                     ESVGLPRSAARFLNLPFYRTGEVRKHPIGADDVRIVRALLEEVRPDYVFVAGDLSDPH
                     GTHRMCKAAIEHALSETPELRPEVWLYRGAWQEWTIPEADLLVPLSQDELRAKILAIF
                     KHQSQKDTAPFPGAHDEREFWQRVEARNLETAAHADRLGLSEYFAMEAYRVERGSGD"
     gene            4387..>4706
                     /locus_tag="AC16JC_02605"
     CDS             4387..>4706
                     /locus_tag="AC16JC_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163618.1"
                     /translation="MSITVLLVLALAFAVVLALQAVGASQHQKAQAERVMRDYSAFAA
                     ARYASRSAGYLWYYAFTPAIKAIDHARGRDGRLPDPSHLKPPADTSQDSLSVHLLRRI
                     QTAF"
BASE COUNT          726 a         1696 c         1605 g          679 t
ORIGIN      
        1 gtgcagcgcg gcagcgtgat ggacatgccg acctacccgt gtgacccgtt gagccccggc
       61 tgggcggcgg tcgcgggcgg gcggcggctc acccaggcgg aggcgacgac gctcgagaag
      121 atccccgtgc tcccgatcgg ttacggggac gccctgccgc tgctgcgcac cctgaccgga
      181 cccgtggcac cggaatcgtg gcgcggctcc ctgccgctca cctaccacat cggccccggc
      241 acgacgaagg tgcatctcgc gctgagcttc gagtggcgca gccggccgct gtacgacgtc
      301 atcgccacca ttcccggcgc ggtctggccc gaccagtggg tgatctatgg caaccaccac
      361 gacgcctggg tgaacggggc cgaagacccg atttcggggc aggcggccct gggggagacg
      421 gcacgggcgc tggccacgct cctcaagacc gggtggcggc cggcccgcac catcgtgctc
      481 gccgcctggg acggagagga gtgggggctg ctcggctcca ccgagtgggc ggaggcgcac
      541 gccggcgagc tgcgcaccaa ggcggtcgtc tactacaact ccgacaccaa cggcgcgggg
      601 tggatcggcg ccgcgggctc ccacgcgctg gagcagttca tcaccgaggt cgcccgcgac
      661 gtcaccgacc cgctgaccgg caagagcgcg ctccaggcct cgctcgatca ccggcgggcg
      721 gagcggacgc cgcgcgacac gagccgcgac acgaccttcg cccttggtgc gctcggctcg
      781 ggctcggact acaccgcctt catcgactac ctcggcctcc cgtcggtgaa cctcagctac
      841 ggcggggcgg cgcacagcgg gatctaccac tcgatctacg acagctacac gttctacgag
      901 cgcttcctcg acacggggta cgtgtacggt gtgaccgagg cgcagaccat ggccacggcg
      961 gtcctccgca tggcggatgc gccgctgctt cccttcgagt tccgcgcccc gacgcgcacc
     1021 taccgccgct acgccgacga aatcgcgaga ctggcggcga agaacgacac caccaaggga
     1081 ctcgacctgg cggcgctgcg caccgccctc gaccgcctcg acacggcggc cggctcctgg
     1141 gagcgcgagc gtgcggcatt cgatgccgtc cggggcaggg acgccgccag gcgccgggct
     1201 ggcctggggg cggtgaaccg gttgctcgcc tcgagcgagc aggcgctgac cgattccgcg
     1261 gggctgccgc ggcggccgtg gttccggcat ctgatctacg cgccggggtt ctacaccggg
     1321 tacggggtga agaccatgcc gggcatccgc gaggcggtgg agcaggcgga cgcaacggag
     1381 gcgcaggcgc aagcggcgcg ggtggcggcg gcgctcacgc ggtatgccga cgtcgtgcac
     1441 cgcgcggcgg acgctctggc ggcggcactg cggtgaaacc gggctgtatt gacacccccc
     1501 gtccggccgg gtaccgtggg gcgaccctaa tcgcaggctt cagccgccgt ccatgagcgc
     1561 cccactcccg ccccgcccat ccatgccgcg gtcgtcgttc gcgatcgcga tgaaggacta
     1621 cactctcatc cggccgggta tcgccatcac gatcagtgcg gcgacgctgc tggcggcgct
     1681 gatcattggc gtgctgctga tccggtcgct cgccagcgcg tcggcgacgg tgaaccgggg
     1741 cgcgacggcc agcgaggcgc tgcaccgcct gaatgcgaag tatgaggtct ggcggaccat
     1801 ggcgacgggc ggcgacacga cctaccgcgc ccaggcggac agtatccgcg gctcgctgca
     1861 gacggatctg tcgacgttca gcacgtccct cgccaacgag caggagcgga gctacgtgca
     1921 gcagatcctg gaaggcctga acaacacgga cgctccgacc acgaagaacg cccgggaagc
     1981 gatgatcgtc ctgctcgccc accaggatgc cgcgctgttc gccgccgccc ggacctcgca
     2041 gcagggagtc gtattcgccg ccgtgctgct cgccctgacc atcctcgcgg ccggcatgct
     2101 gatcgtcccc atggcgtggc tgtacgtccg gtacaagcgc ggggcgacga tcgaagtgaa
     2161 gatgtagatg gccggcgggc tggaaggccc gcgggaccgg gaacggctgc gcaccgtcgt
     2221 cgtcgccgat cccgacgatc tggccgctct gctggcggac cggatcgtcg cggcgatccg
     2281 ccgcgacacg gccgccaagg ggcgctgcgt tctgggcctc gccacgggga gcacgccgct
     2341 gggcgtctac cgtgagctga tccgccggca ccgcgccggc gaagtggaat tctcccgcgt
     2401 catcgcgttc aatctcgacg agtacttccc gatgtcgccg gacagcccgc acagctaccg
     2461 gcgctacatg tgggagaact tcttcgcgca cgtcgggatg cggccggagc agacgcacat
     2521 ccccaccgga gacacgccgc gcgacgggat cgcccaggcc tgcgccgact acgagcgatc
     2581 catcgtcgat gccgggggca tcgactttca gctgctcggc atcggccaga gcgggcacgt
     2641 cggattcaac gagcccgggt cggcgcgcga gtcgcgcacg cggctcgtca tgctcgacgc
     2701 cctcacgcgc aagagcgccg cggcggattt cttcggcgaa gacaacgtgc cgcgcgaggc
     2761 gatcaccatg ggcgtcgcga ccatcctggc cgctcgcgag atcgcgctcg tcgccaccgg
     2821 cgagcacaag gcggtgatcg tgcgccgcgc cgtggagggc gagccttccc ccgacgtcgc
     2881 cgcgacctac ctgcagggtc atcccgacgc gacggtctac ctcgatctcg cggccgccgc
     2941 cgaccttacg cgcatccgga ccccctgggt gctggggccc gtggagtgga caccggcctt
     3001 gacggagcgg gccgtggtct ggctcgccga gcgtaccgac aaggcgattc tcaagctgac
     3061 ggccgcggac tacaccgcgc accacctgag cccgttgctc gcccaggccg gcgccgccgg
     3121 tccgatcaac ggcctggtct tcaaccggct gcgcgacaag atccgggggc ggcgcaagct
     3181 ccccgcgggg cggcgcgtgc tcgtcttctc gccccatccc gacgacgacg tgatctcgat
     3241 gggcgggatc ctgcgcaagc tgcacgagaa cacgaacgag atcaccgtcg cctacatgac
     3301 gagcggcaac atcgccgtgt tcgaccacga cgtgcggcgc cacctcgatt tcgtagagcg
     3361 ggcggccgcc gccctggagg tcgaccccgc cgccgtgcgg cgcgtgagcc gggcggtact
     3421 gtcccagtac gagcgcaaga ccccgggcga cgtggacctg ccggtcgtgc aggatctcaa
     3481 acgcttcatc cgggaatcgg aagcgatcgc ggcgctcgaa tcggtgggcc tgccgcgcag
     3541 cgccgcgcgg ttcctgaacc tgcccttcta ccggacgggc gaagtccgca aacaccccat
     3601 cggagccgac gacgtgcgta tcgtccgcgc gctgctcgag gaggtgcgtc ccgattacgt
     3661 gttcgtcgcg ggcgatctct ccgatcccca cggcacccac cggatgtgca aggcggccat
     3721 cgagcacgcc ctgagcgaga cgcccgagct ccgccccgag gtctggctgt accgcggcgc
     3781 ctggcaggaa tggacgattc ccgaagcgga cctcctggtg ccgctgtcgc aggacgagct
     3841 gcgcgccaag atcctcgcga tcttcaagca ccaatcccag aaggacacgg cgccgtttcc
     3901 cggcgcgcac gacgagcgcg agttctggca gcgcgtcgag gcgcgcaacc tcgagaccgc
     3961 ggcgcacgcc gatcgcctcg gcctctccga gtacttcgcc atggaggcgt accgggtgga
     4021 gcgcggctcg ggagactagc ccccacgcgg ctccggccca cccgcccgga aataccaaac
     4081 ccggcgctga aagctcgccg ggctggtcgg tcgggtgagg tggccgtcac acctcgatcc
     4141 acgacctgag agagaaccga tttatttacg tccggcctat cctaccgcgc cccgctctgt
     4201 tggttccagc tacgcgaggc gtgcggcctg tgacgcgact gcaatgtggc gcaagtgcgg
     4261 cgttgccggg tggagatggc gtgtgccccc tgtcagggtt cggtcaaccc accctccggc
     4321 ctgacacatt agaagttagc attcttcgcc atgcggctcc cgcgctcccc ccgactgccc
     4381 cgtacggtgt cgatcaccgt gctgctggtg ctcgcgctcg cgtttgcagt ggtactcgcg
     4441 ctgcaggcgg tgggcgcctc acagcatcag aaggcgcaag ccgagcgcgt gatgcgcgac
     4501 tactccgcgt tcgcggctgc gcggtatgct tcgcggtccg caggctatct ctggtactac
     4561 gccttcacgc cggcgatcaa ggcgatcgac catgcgcgcg gccgcgacgg ccgactcccc
     4621 gatccttcgc acctcaaacc accggcggac acgtcgcagg attccctctc ggtgcacctg
     4681 ctgcgccgca tccagacggc gttcga
//
LOCUS       DBNGZW010000107         2729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1578584, whole genome shotgun sequence.
ACCESSION   DBNGZW010000107 DBNGZW010000000
VERSION     DBNGZW010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2729
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1578584"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..612)
                     /locus_tag="AC16JC_02610"
     CDS             complement(52..612)
                     /locus_tag="AC16JC_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR5163619.1"
                     /translation="MKRIAFGILALLSVAGVGGLSAQNIRFGVGGGLVLPMGDYKDVD
                     KLGWLVGADATYWLTGDAVGIRLEGSYSQTKHKDFAGSPVDGNSKIIGGMADLVYAFG
                     TQADQIRPYVLGGVGFFNVKVTVPSASFDTSVTKVGFGGGAGIDYKVGTGSTRVFVEA
                     RYMSVSTEGGSTNFLPIRAGLRFCGK"
     gene            complement(639..896)
                     /locus_tag="AC16JC_02615"
     CDS             complement(639..896)
                     /locus_tag="AC16JC_02615"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF014838.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta/epsilon"
                     /protein_id="HIR5163620.1"
                     /translation="MRVTLISPDRSLFDGAASGVVAPAFDGFVGILPGHAPFLTLLGT
                     GTLTITNSGAASRFAVDGGILQVVEDVVRVVAERAEPSATT"
     gene            complement(896..2362)
                     /gene="atpD"
                     /locus_tag="AC16JC_02620"
     CDS             complement(896..2362)
                     /gene="atpD"
                     /locus_tag="AC16JC_02620"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011429328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR5163621.1"
                     /translation="MATKTQNVTKKSAGATKQNVGKVIQVIGPVLDVEFEPEHLHEIY
                     NALVVDQPGGSGAPQLRLVAEVQQHIGRNQVRAVAMTSTDGLVRGMPAVDTGGPITVP
                     VGKAALGRILNVLGDPVDEGPPIPADAERWPIHRETPKFVDLEPKTEVFETGIKVVDL
                     IAPFVKGGKIGLFGGAGVGKTVIIQELIHNVALGHGGRSVFCGVGERTREGNDLFLEM
                     KESGVIGSCALIYGQMNEPPGARLRVGLSGLTVAEYFRDVEGQDVLVFIDNIFRFTQA
                     GSEVSALLGRMPSAVGYQPTLATEMGELQERITSTRKGSITSVQAIYVPADDLTDPAP
                     ATAFSHLDATVVLDRAIVELGIYPAVNPLSSSSRILDAQYLGDRHYKVAVEVQRILQR
                     YKDLQDIIAILGMDELSEEDKILVGRARRVQRFLSQPFFVASQFTGREGKYVKLEDTI
                     ASFERVVAGEFDHLPEQAFYMVGGIEDVVEQAKQLAAA"
     gene            complement(2435..>2729)
                     /locus_tag="AC16JC_02625"
     CDS             complement(2435..>2729)
                     /locus_tag="AC16JC_02625"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR5163622.1"
                     /translation="VHTPTARPPDRPFFILRPSAAAILEQLLPLYVRNQVYRALVETA
                     AAEHGARRTAMKNATDSAGEMLDLLRRTFNRARQAQITQEIAEIVGGAEALQG"
BASE COUNT          464 a         1001 c          810 g          454 t
ORIGIN      
        1 cccccggcgg agcggagtcc gccggggggt ctggaacgcg tgaatcggcg tttacttgcc
       61 gcagaaccgg agaccggcgc gaatcggcag gaagttcgtc gagccacctt ccgtgctcac
      121 actcatatac ctggcctcaa caaagacacg cgtgctcccc gtgccgacct tgtagtcgat
      181 cccggccccg ccgccaaacc cgaccttcgt cacggacgtg tcgaaactcg ccgacgggac
      241 agtcactttc acgttaaaga aaccaacccc cccaagtaca tagggacgaa tctggtccgc
      301 ctgggtgccg aaggcataca cgagatcggc catcccgccg atgatcttcg agttgccgtc
      361 aacggggcta cccgcaaagt ccttgtgctt ggtttggctg taactgccct cgaggcgaat
      421 gccgaccgca tcccccgtca gccaatacgt cgcgtcagcg ccgacgagcc accccagttt
      481 gtccacgtcc ttgtaatcac ccatcggcag caccagacca ccgcccacgc cgaaccgaat
      541 gttctgcgcc gacagcccgc cgacgccggc cacgctcagc agcgccagaa taccaaatgc
      601 gatgcgcttc atgacacccc tccagggttt gatgtccatc aggtagtcgc cgagggctcc
      661 gcccgctcag ccaccactcg cacgacgtcc tcaaccacct gcagaattcc gccatcgacg
      721 gcaaaccggc tcgccgcgcc gctgttcgtg atcgtcaacg tccccgtccc gagcagcgtc
      781 aggaacggcg catgccccgg caggatgccc acgaacccgt cgaacgccgg cgccaccacg
      841 ccgctcgccg caccgtcgaa cagcgagcga tccggcgaga tcagcgtcac ccgcatcagg
      901 ccgccgccag ctgcttggcc tgctccacca cgtcctcgat cccccccacc atgtagaatg
      961 cctgctcggg gagatgatcg aactcgccgg cgaccacgcg ctcgaacgag gcgatcgtgt
     1021 cctcgagctt cacgtacttg ccttcgcggc cggtgaactg cgacgccacg aagaacggct
     1081 gcgacaggaa gcgctgcacc cgccgcgccc ggccgaccag gatcttgtct tcctccgaca
     1141 gctcgtccat gccgaggatc gcgatgatgt cctgcagatc cttgtagcgc tgcaggatac
     1201 gctgcacttc caccgccacc ttgtaatgcc gatcgcccag atactgggcg tcgagaatac
     1261 ggctcgacga cgacagcgga ttcaccgcgg gatagatgcc gagctcgacg atggcgcgat
     1321 ccagtaccac cgtcgcgtcg aggtgcgaaa acgcggtggc cggcgccggg tcggtgaggt
     1381 cgtcggccgg gacgtagatc gcctgcaccg acgtgatcga gcccttccgg gtcgaggtga
     1441 tgcgctcctg cagctcgccc atctcggtcg cgagtgtcgg ctggtacccc acggccgacg
     1501 gcatccggcc cagcagcgcc gacacctcgg agcccgcctg ggtgaagcgg aagatgttgt
     1561 cgatgaagac cagcacgtcc tggccctcga cgtcgcggaa gtattccgcc accgtcagcc
     1621 cgctcaggcc cacccgcagc cgggcgccgg gcggctcgtt catctgaccg tagatcagcg
     1681 cacaggagcc gatcacgccg ctctccttca tctcgaggaa caggtcgttc ccttcgcgcg
     1741 tgcgctcccc cactccgcag aagaccgagc ggccgccgtg ccccagcgcg acgttgtgga
     1801 tcagctcctg gatgatcacc gtcttgccga cgcccgcgcc gccgaacaag ccgatcttgc
     1861 cacccttcac gaacggcgcg atcagatcga ccaccttgat gccggtctcg aacacttcgg
     1921 tcttcggctc gagatccacg aacttcggcg tctcgcgatg gatcggccag cgctcggcgt
     1981 ccgccggaat cggcgggcct tcgtccaccg ggtcgcccag cacgttcagg atacgcccca
     2041 gcgccgcctt ccccaccggg accgtgatcg ggccgcccgt atcgacggcc ggcatgccgc
     2101 gcaccaagcc gtcggtcgag gtcatcgcca ccgcccgcac ctggttgcgg ccgatgtgct
     2161 gttgcacttc ggcgaccagg cgcagctgcg gggcgccgct gccgcccggc tggtccacca
     2221 ccagggcgtt gtagatctcg tgcaggtgct cgggctcgaa ctccacgtcg agcaccgggc
     2281 cgatgacctg gatcaccttg ccgacgttct gcttcgtcgc gcccgcggat ttcttcgtca
     2341 cgttctgcgt cttcgtcgcc atgttggttc cttgaggtgt cgggtgccgg gtgtcgggtg
     2401 ttaggcctga aacccgacac ccagcacctg acacctatcc ttgcagcgcc tccgccccgc
     2461 ccacgatttc cgcaatctcc tgcgtgatct gcgcctgccg cgcccgattg aaggtgcggc
     2521 gcaggaggtc gagcatctcc cccgccgaat ccgtggcgtt cttcatcgcg gtgcgccgcg
     2581 ccccgtgttc ggcggccgcc gtctccacca gcgcacggta cacctggttg cgcacgtaga
     2641 gcggcagcaa ctgctcgagg atcgcagcgg ccgacgggcg caaaatgaaa aatgggcggt
     2701 caggcggtcg ggcggtcggt gtgtgcacg
//
LOCUS       DBNGZW010000108         3187 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_281852, whole genome shotgun sequence.
ACCESSION   DBNGZW010000108 DBNGZW010000000
VERSION     DBNGZW010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_281852"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..274
                     /locus_tag="AC16JC_02630"
     CDS             <1..274
                     /locus_tag="AC16JC_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5163623.1"
                     /translation="GEEVHESTDQFFRVEEGTGEVSIDGRRTQVEGDDAIVIPAGARH
                     NIRNIGAEPLKLYTLYAPPQHEDGTVHRTKKDAERLEEHFAGRTTE"
     gene            complement(403..918)
                     /locus_tag="AC16JC_02635"
                     /pseudo
     CDS             complement(403..918)
                     /locus_tag="AC16JC_02635"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
     gene            complement(939..1250)
                     /locus_tag="AC16JC_02640"
     CDS             complement(939..1250)
                     /locus_tag="AC16JC_02640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163624.1"
                     /translation="MLLVREIMYCKPGKVRPMVEKFLAMNKLSAKVGMPQMRVMTDFC
                     AERYWTLVAEMEVESMADFEKMMQGGGQDAAAMKEMETLMKDYHDLVEYGRREVYKIE
                     G"
     gene            complement(1357..2316)
                     /locus_tag="AC16JC_02645"
     CDS             complement(1357..2316)
                     /locus_tag="AC16JC_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018091548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR5163625.1"
                     /translation="MLILSHRQVEELLDLDVLIEALAVAMGDLSAGRASVPRRNFAMA
                     GASGILAAMPAYLPTQNVLAAKLVLVFPGNASKGLETHQAVVAVFDPATGVPLALMDG
                     ASITAIRTAAGSALATRLCAREGAKVLAILGTGVQARSHAQAVPRVRNITEIVVAGRT
                     PEKVAAFAAEIGARAASTYAEAVVAADIVCCCTNATEPVVRREWLRPGAHVNSVGFTK
                     GPELDPRVFADALVVVESRTAAIGEFPNGAVDITAAVNQHILQLADIREVGELVQGRG
                     LSRTGEDQVTVYRSVGVAAQDAAAAGLVLEAARARGMGADARL"
     gene            complement(2303..>3187)
                     /locus_tag="AC16JC_02650"
     CDS             complement(2303..>3187)
                     /locus_tag="AC16JC_02650"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5163626.1"
                     /translation="DSLFALRAAIRSGARIGPDLVLAGSTLNGEQSAPFHRKVVTDSE
                     ARIAVHELAAAGVDFIKVHRATGREAFLAILQEARGLDLFVCGHVPLALSWIEASNLG
                     MRTIEHIQTIYENEQSDPHLVAAQFTAIADHLDGPYGDSIFAVLVRNHTFFDPTLIGY
                     EVSIDRAAPPVAALRRAAYARMQALAGRAARDGVRLLAGTDVLERHGDMLLTELERLV
                     AIGLTPQQALAAATTTAAEAMSRGSGRIEPGAPASFLIVDANPLSDIRNLRVLSAVVL
                     RGRLIEAGELEALRHADP"
BASE COUNT          499 a         1165 c         1037 g          486 t
ORIGIN      
        1 cggcgaagag gtgcacgaga gcacggacca gttcttccgt gtagaagaag ggacgggcga
       61 ggtctcgatc gacggacggc gaacccaggt cgagggtgac gatgccatcg tcattcccgc
      121 cggcgcgcgg cacaacatca ggaacatcgg agccgagccg ctgaagctgt acacgctgta
      181 cgctcccccc cagcacgagg acggcacggt gcatcgcacc aagaaggatg ccgaacggct
      241 ggaggagcac ttcgccggca ggacgaccga gtaggcccta tcccccggcc gacgacggac
      301 gggcggccgg gggttttcgc cgatcaactg ccgcatcgcg cggccgccgg cgccgtggcg
      361 cagagtgatc acggcgtcgc ccccgcgggc cgtcgtggtc gctgtcacgt tgtcggtcgt
      421 ccagccccag cgccgcgtca ggtaaccgaa gccgccgccc agggtgagtc ccgcgatgcc
      481 ggtcgcggac acgaacccga gcacggcagc gaggccatgc acctgcgtct cccggtccac
      541 gtcgccgagc agacagccgg cctgcgcccg ggcgatcctt ctctgtggat ccgcccacac
      601 gccgcgcatg agcgacatgt cgagcatcag cgcgccgtcg gccgtcgcca ggccggcaat
      661 gttgtgccct ccacccttga tgcagagcag caggtcgtgc gcccgtgcga agcggacgca
      721 ttcaatcacg tcgccggtcc cgagacagcg cacgaccagg gccggcctgc ggtcgatcat
      781 ggcgttccag accgtccgcg actcctcata gcccgcgtcg ccgggcgcgc agaccggacc
      841 acgcaggcgc gacctgagcc cgtcgagcgc gtcctgcttc aggtccgtct cgcggccgcc
      901 ggtcgtcctg gctttcatcg tttcgttcgc gtcgcccctc acccctcgat cttgtagacc
      961 tcgcgccgcc cgtattccac gagatcgtga tagtccttca tgagcgtctc catctccttc
     1021 atcgccgcgg cgtcctgccc gcccccctgc atcatctttt cgaagtcggc catgctctct
     1081 acttccatct cggcgacgag cgtccaatac cgctcggcac aaaagtcggt catcacccgc
     1141 atctgcggca tcccgacctt cgcgctgagc ttgttcatgg cgaggaactt ctccaccatg
     1201 gggcgcacct tgccgggctt gcagtacatg atctcgcgaa cgagcagcat acggtcctcc
     1261 tcgtcgttgg ccttcagggt cgtggctggg tgggctgcca ttcaggaagc gactaacctg
     1321 ccgtctgtcg cggcggccag caagggacgc gatcgactag aggcgcgcgt cggctcccat
     1381 ccccctcgca cgcgccgcct cgaggacaag cccggcggcc gcggcgtcct gcgccgccac
     1441 gccgaccgag cggtacacgg tgacctgatc ctcgccggtc cgcgataacc cccgtccctg
     1501 caccagctct ccgacctcac gtatgtccgc cagttgcagg atgtgctggt tgacggccgc
     1561 ggtgatatcc acggcgccat tgggaaactc gccgatggcg gcggttcgcg attcgaccac
     1621 caccagcgcg tcggcgaaga cccgcggatc gagctccgga cccttggtga atccgaccga
     1681 attcacgtgc gcccccggcc gcagccactc gcgtcggacg accggctcgg tcgcattcgt
     1741 acagcaacag acgatgtcgg ccgccaccac cgcctcggcg tacgtcgatg cggccctggc
     1801 gccgatctcc gccgcgaagg cggccacctt ctccggtgtt cgtccggcca ccacgatctc
     1861 ggtgatgttg cggacgcgcg ggaccgcctg cgcgtgcgat cgcgcctgca ccccggtccc
     1921 gagaatcgcg agcaccttcg ccccttcccg ggcgcacagt cgcgtcgcca gtgccgaacc
     1981 ggccgccgtg cggatcgcgg tgatcgatgc gccgtccatg agggccaggg ggacaccggt
     2041 cgcgggatcg aacaccgcga ccacggcctg gtgcgtctcg agccccttcg atgcgttgcc
     2101 cgggaatacc aggacgagct tggctgccag cacgttctgg gtggggagat acgccggcat
     2161 cgcggcgaga attcccgagg ccccggccat cgcgaagttc cggcggggca ccgaggctcg
     2221 acccgcgctg aggtccccca tcgccacggc gagcgcctcg atcagcacat ccagatcgag
     2281 cagctcttcc acctgtcggt gactaaggat cagcatgccg gagcgcctcc aactcgccgg
     2341 cctcgatcag ccggccgcgc agcacgaccg cggacaacac ccggaggttc cggatgtcgc
     2401 tcaggggatt cgcgtccacg atgaggaacg acgccggggc gccgggctcg atccgaccgc
     2461 tgccgcgcga catcgcctcg gccgccgtgg tggtcgcagc cgcgagcgcc tgctgcggcg
     2521 tgagcccgat cgccaccagt cgctccagct cggtgagcag catgtcgccg tgccgctcga
     2581 gcacatccgt acccgccagg agccgcaccc cgtcgcgcgc ggcccggccg gcgagtgcct
     2641 gcatgcgggc gtaggcggcc cgtcgcagcg ccgcgaccgg cggcgcggcc cggtcgatcg
     2701 agacctcgta cccgatcaga gtgggatcga agaacgtgtg gttgcgcacc agcacggcga
     2761 agatcgaatc gccgtacggc ccgtccaggt ggtcggcgat cgcggtgaac tgcgctgcca
     2821 cgaggtgcgg atcggactgc tcgttctcgt agatcgtctg gatgtgctcg atcgtccgca
     2881 tgccgaggtt cgacgcctcg atccagctca gcgcgagcgg cacgtgcccg cagacgaaaa
     2941 ggtccagtcc ccgcgcctct tgaagaatcg ccaggaacgc ctcgcggccg gtggcgcggt
     3001 gcaccttgat gaagtcgacg cccgccgccg cgagctcgtg caccgcgatg cgggcctcgg
     3061 agtcggtgac caccttccga tggaacgggg cggattgctc tccgttgagg gtggaccccg
     3121 ccagcaccag atcggggccg atccgtgcgc cggaccggat cgcggccctc agcgcgaaca
     3181 gcgagtc
//
LOCUS       DBNGZW010000109         3738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1305378, whole genome shotgun sequence.
ACCESSION   DBNGZW010000109 DBNGZW010000000
VERSION     DBNGZW010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3738
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1305378"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="AC16JC_02655"
     CDS             <1..263
                     /locus_tag="AC16JC_02655"
                     /inference="COORDINATES: protein motif:HMM:NF036531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HIR5163627.1"
                     /translation="GAEGAGWYYGYWEYGWWSPWDHRELRDDRVVYVATTLWPGTYTM
                     TYVARATTPGVFVRPPVYAEEMYNPGVNGRSDGGLFTVTAKP"
     gene            277..1134
                     /locus_tag="AC16JC_02660"
     CDS             277..1134
                     /locus_tag="AC16JC_02660"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015141275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5163628.1"
                     /translation="MRLIRALKAEDAPEFERFLGEYADSSMFLRSNARAGGLNYEGKP
                     LQADYVAAFAGGRIVAVAAHCWNGMVLLQAPHPEELGAVARAAVAHSGREVTGISGPA
                     DQVAAARTALGLDDRPAPKFGREELFALSLAELVVPAPLADGLWTSRPPRDDELDLVT
                     GWRVDFMVEALHRAVGAGLRDEAAAEVRLLHALKLDWLLLDAGRPVAFSAFNAALPDI
                     VQVGGVWTPPALRGRGYGRAVVAGSLLDARARGVTRAVLFAEREDAKRAYVGIGFRAV
                     GEYGLLLFD"
     gene            1246..1677
                     /locus_tag="AC16JC_02665"
     CDS             1246..1677
                     /locus_tag="AC16JC_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HIR5163629.1"
                     /translation="MSAGYSSRSLPDKLGIKPNTRIALLNAPRGYGVTLRPLPPGLRV
                     TGSLRGTFPFIQLFTSSRRDLERRFPALFRALAQDGALWISWPKKSSGVVTDLTEDVI
                     RAVALAGGLVDVKVCAVDAVWSGLKLVRRKRDRLRPVADGR"
     gene            1714..2325
                     /locus_tag="AC16JC_02670"
     CDS             1714..2325
                     /locus_tag="AC16JC_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163630.1"
                     /translation="MTESPKLPPSMASPQPLADRALDNLRYIRATMERAGSFTAVPGW
                     GMVAIGTSALAASFIAARQASFLDWLVAWLVEAMLALAIGGWTMVRKSRTANDPILTG
                     PGRRFGLSFLPPVAVGALLTVALYLGGDRRLIPAVWLLLYGTGVVTGGAFSVPVVPVM
                     GVGFLALGGAALFAAGAANWLLAAGFGGLHIVFGLWIARRYGG"
     gene            2318..2683
                     /locus_tag="AC16JC_02675"
     CDS             2318..2683
                     /locus_tag="AC16JC_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5163631.1"
                     /translation="MAKSNAIRHPAATSGAAASARGAARAAAELDRLIHERVRLGIVA
                     ALAGVERASFNELKSLLKVSDGNLSVHARKLEDADYIACTKSFEGRTPRTEYRLTATG
                     RRALERYLDHMEALIRATR"
     gene            2712..3338
                     /gene="uppS"
                     /locus_tag="AC16JC_02680"
     CDS             2712..3338
                     /gene="uppS"
                     /locus_tag="AC16JC_02680"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HIR5163632.1"
                     /translation="MTQSSLHVAIIMDGNGRWAERRGLDRTVGHRVGATAVRRVVEAA
                     PAQGVTALTLFAFSSDNWKRPAHEVSALMRLFARHLRTETARCMEEGVTLRVIGRRDR
                     LPGPLVEAIERSEAATTGGARLELRLAVDYSARHAIETGRLIPDVDLLIRTGGEQRLS
                     DFLLWECAYAEILFTQTLWPDFGAADLAAAVSWFHGRERRFGGLKESA"
     gene            3335..>3738
                     /locus_tag="AC16JC_02685"
     CDS             3335..>3738
                     /locus_tag="AC16JC_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163633.1"
                     /translation="MITDSMGAVSFGLFIVAVLGTLALGAGYVLALLLKRAVWARWIL
                     RIELAGLALYLVLFLLASFTSGTRELAVGEEKHICEVDCHLAYAVTGTRTARQWDGKT
                     AQGTFYVVTVRVRFDSGTIAPWRPRNVPLSPNG"
BASE COUNT          506 a         1269 c         1336 g          627 t
ORIGIN      
        1 ccggcgccga gggagcgggc tggtactacg ggtactggga gtacggctgg tggtcgccgt
       61 gggaccaccg ggagctgcgc gacgatcgcg tggtgtatgt cgccaccaca ctctggcccg
      121 ggacatacac catgacttac gtggcgcgcg ccacgacgcc cggcgtgttc gtgcggccgc
      181 ccgtgtacgc ggaggagatg tacaacccgg gggtgaatgg gcgcagcgac ggcgggctct
      241 tcacggtgac cgcgaagccg tgagccgagt gggttcgtgc ggttgatccg agcactcaag
      301 gcggaagacg cgccggaatt cgagcggttt ctcggggagt acgccgactc ctcgatgttc
      361 ttgcgctcca acgcgcgtgc gggggggctg aactacgagg gcaagccgct gcaggcggac
      421 tacgtggcgg cgttcgcggg cgggcggatt gtcgccgtcg ccgcgcactg ctggaacggg
      481 atggtgctgc tgcaggcgcc ccatccggag gagctggggg ccgtcgcacg cgcagcggtg
      541 gcccattccg ggcgggaggt cacggggatt tcggggccgg ccgatcaggt ggcggccgcg
      601 cgtaccgcgc tgggactcga cgaccgcccc gcccccaaat tcggtcgcga ggagctgttc
      661 gccctcagcc tcgccgaact cgtcgtgccc gccccgctgg ccgacggcct ttggacttcc
      721 cgtccgcccc gggacgacga gctcgacctc gtgacgggct ggcgcgtgga cttcatggtc
      781 gaggcgctgc atcgcgccgt gggcgcggga ctgcgcgacg aggccgcggc ggaggtgcgc
      841 ctgctgcatg cgctgaagct ggactggctc ctcctggacg ccggacggcc cgtcgccttc
      901 tccgcgttca acgcggcgct gcccgacatc gttcaggtcg gcggcgtgtg gacgccgccc
      961 gcgctgcgcg gccgcggcta cgggcgcgcc gtggtggcgg gctcgctgct cgacgcccgc
     1021 gcccgcggcg tcacgcgcgc cgtgctgttc gccgagcggg aagacgccaa gcgggcatat
     1081 gtggggattg ggttcagggc ggtgggcgaa tacggattgt tgctcttcga ctgaccaccg
     1141 gatccgcacc caacttgccc caccccgcgg gctatcagct atcggctatc ggctatcggt
     1201 tagaccgata gctgacagcc gacagccagt agcgcatgtt tcggcatgag cgccggctac
     1261 tcgtcacgga gcctcccgga caaactgggc atcaaaccga acacccgcat cgctctcctc
     1321 aatgcgccgc gcgggtacgg cgtcacgctc cgcccccttc cccccgggct gcgggtaacg
     1381 gggagtctgc gaggcacgtt cccgttcatc cagctcttca cgtcgtcccg ccgggatctc
     1441 gagcggcgct ttccagcgct gtttcgggcc ctggcccagg acggggcgct gtggatctcc
     1501 tggcccaaga agtcctcggg agtggtcacg gatctgaccg aggacgtcat ccgggccgtg
     1561 gcgctggccg gaggtctcgt ggacgtaaag gtttgcgccg tggacgcggt gtggtcaggt
     1621 ctcaagctgg tgcgccgcaa gcgggaccgg cttcggccgg tagccgatgg ccgctagccg
     1681 atagcacttt attgtacaaa gtacttgaca gaaatgaccg aatcccccaa attgcctccc
     1741 agcatggcgt cgccgcagcc gctcgccgat cgcgcactcg acaacctgcg ctacatccgg
     1801 gccacaatgg agcgcgcggg atcgttcacc gcggtgcccg gctggggcat ggtggcgatc
     1861 ggcacgagtg cgctggccgc gtcgttcatc gccgcgcgtc aggcgtcctt cctggactgg
     1921 ctcgtcgcct ggctggtgga agcgatgctg gcgctggcca tcggtggctg gacgatggtc
     1981 cggaagtccc ggacggcgaa cgatccgatc ctgacgggcc cggggcgtcg cttcggcttg
     2041 agcttcttgc ccccggtggc ggtgggcgcc ctgctgacgg tggcgctgta cctcgggggt
     2101 gatcggcgac tcatcccggc cgtgtggctg ttgttgtacg gcaccggggt cgtgacgggc
     2161 ggcgccttct cggtgccggt ggtgccggtg atgggcgtcg ggttcctggc gctcgggggg
     2221 gcggcgctgt tcgccgccgg tgcggcgaac tggctgctcg ccgcggggtt cggcggcctg
     2281 catatcgtct tcggactgtg gatcgcgcgg aggtacggtg gctaaatcga acgcaatccg
     2341 gcatcccgcc gccacgagcg gcgccgcggc ctcggcccgc ggcgcggccc gcgctgcagc
     2401 cgaactcgat cgcctgatcc acgagcgcgt ccgcctgggc atcgtggcgg cgctcgccgg
     2461 tgtggagcgg gccagcttca acgagctcaa gagtctgctc aaggtctccg acggcaacct
     2521 cagcgtgcat gcgcgcaagc tggaggacgc cgactacatc gcctgcacca agtcgttcga
     2581 gggccgcacg cctcgcaccg aataccgcct gaccgccacc ggccgccggg cgctggagcg
     2641 gtatctcgat cacatggaag ccctgattcg cgccacgcgc tgacgggagt tttttttcga
     2701 ggtcttactt tatgacacaa agttctctgc acgttgcgat catcatggat ggaaacggcc
     2761 gctgggccga gcggcgtggt ctcgaccgga ccgtgggcca ccgcgtcggc gccaccgccg
     2821 tgcgccgcgt cgtcgaggcg gcgccggcgc agggtgtcac cgcgctcacg ctgttcgcgt
     2881 tctcctccga caactggaag cgcccggcgc acgaagtgag cgccctgatg cggctcttcg
     2941 cccgccacct gcgcaccgaa acggcgcgtt gcatggaaga gggcgtgacg ctgcgcgtca
     3001 tcgggcggcg cgaccggttg cccggcccgc tggtcgaagc catcgagcgc agtgaagcgg
     3061 cgaccaccgg cggcgcacga ctcgaattgc ggctcgccgt ggattactcc gcccgccacg
     3121 ccatcgagac gggccggctc atcccggacg tggatctgct gatccgtacc ggcggcgagc
     3181 agcgcctctc cgatttcctg ctgtgggagt gcgcctacgc cgaaatcctg ttcacgcaga
     3241 cgctgtggcc cgacttcggc gccgcggacc tcgcggccgc cgtgtcctgg ttccacggcc
     3301 gggagcgccg cttcggcggc ctcaaggaga gcgcatgatc accgacagta tgggcgccgt
     3361 ctcgttcggc ctgttcatcg tcgccgtgct cggcacgctc gccctcggcg ccggctacgt
     3421 gctggcgctg ttgctgaagc gcgccgtatg ggcgcgctgg atcctgcgga tcgagctggc
     3481 cgggctggcg ctgtacctgg tgctgttcct cctcgcgtcc ttcaccagcg gcacccgcga
     3541 gctcgcggtc ggcgaggaga aacacatctg cgaagtggac tgccatctgg cctacgcggt
     3601 gaccggcacg cgcacggcgc gccaatggga cgggaagacg gcgcagggca cgttctacgt
     3661 ggtcaccgtg cgagtccgtt tcgactccgg cacgatcgcc ccctggcgcc cgcggaacgt
     3721 ccccctgtcc cccaacgg
//
LOCUS       DBNGZW010000110         5372 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_9983, whole genome shotgun sequence.
ACCESSION   DBNGZW010000110 DBNGZW010000000
VERSION     DBNGZW010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5372)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5372)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5372
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_9983"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="AC16JC_02690"
     CDS             <1..471
                     /locus_tag="AC16JC_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR5163634.1"
                     /translation="LPDVATVVVPVGGGGLISGVARGLAAAGSPARVVGVEPAGAPKL
                     TRSLEAGRPVTLERTASIADGLITLSVGELPFEVLQQHKERFAGVVTVTDDAIREAVF
                     FLHQRCGLMVEPSGAATTAALRAGLLEQYRRPPMVLVVSGGNVDPALLKDRRRS"
     gene            468..1934
                     /locus_tag="AC16JC_02695"
     CDS             468..1934
                     /locus_tag="AC16JC_02695"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR5163635.1"
                     /translation="MTASSPTSPPPTAAGTKILLADDDESLVQTLSYILKKSGYEVVT
                     SLGGENLLSLVAAERPQLLLLDIMMPKVDGLALLGLLKEDDRFRDLPVLMLSSMDPED
                     AAVRSLGLGASDFIAKPVRARELLARVEAHLRLGKAVAEARGEAKNRAAMVEILHEIT
                     DSLKPEEIYHILVRRVARVLSISKCSIVIAKAGDKMGTVVASFENPMLHNLEIEMARY
                     PEIERALASSSSVLVSDVATDPMYARERTRWQAERILVPTRSAVAIPFNMRDRQVGVF
                     FLRTTEADAALTPADARFAESVIEAAVAAIEKAYDFETAVLDRRRFEQLASTDALTGT
                     MNRRALSEALAAELDRARRYNLYLSILLADLDRFKQINDRRGHLAGDAVLRQVGELLR
                     HEARSVDLVARYGGEEFVVVMPETTLRGAGVFAERVRGKVAVRDFAPAGEEPLQLTVS
                     IGLAGFPNDRVTSADSFIALADQALYRAKNEGRNRVRE"
     gene            2201..3082
                     /locus_tag="AC16JC_02700"
                     /pseudo
     CDS             2201..3082
                     /locus_tag="AC16JC_02700"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
     gene            3092..4183
                     /locus_tag="AC16JC_02705"
     CDS             3092..4183
                     /locus_tag="AC16JC_02705"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR5163636.1"
                     /translation="MKRFPAFDPPEYLSWQPDPALVRAFRQTIEQQPERAALVARLDR
                     DGRRALYAGLVRTRLHDVTLKRWVRTGVISKAWLGTGEEAATVGPVHALERDRDIVAP
                     MIRNAGAVIEMGMPIAQALRAYLATADSPSGGRDLHLGDLAAGVLQPISHVGDVVPVV
                     AGIALTFKMKREPRVALTWVGDGSTKTAAAHEGTNFAAVQRLPAIFIVQNNQVALGTR
                     LEQHHLPGTFKDWAASYGMWGLEFDGNNVLDAWAATKLAAERCRAGQGPALLVAETFR
                     MGGHATHDEREARETFPAKLFAEWGKRDPIGLYEEYLKEDGWTDRELGDVEAEVTAAV
                     ERAEQEALASRGNMPTGEGALSGLFATQG"
     gene            4210..5334
                     /locus_tag="AC16JC_02710"
     CDS             4210..5334
                     /locus_tag="AC16JC_02710"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF048136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR5163637.1"
                     /translation="MQRRMVMAVLFGIACTTGGSVAGQAPAGMVAQEIAGHTLYLRPG
                     FKIGIFADNQRGVRNIVLGPHGAVYAAMQGAGRVLRLVDANGDGVADSVTPAATGLNG
                     PFGIAFRGDTMYVGETSQITRIAPGGAPRPIVTGLPTGGHSTRTVVFGPDGYLYLAVG
                     SSCNICDETDPRRAAVTRFNADGSGEHRFATGLRNSVGLAFSPTTGELWGTNNDRDNI
                     GGSNTSMTDDLPPEHLNIIKDGKFYGWPQCYLPGQHNPEYPSASCANVEPPAITFQAH
                     SAPLGITFYTGSAFPGYTGDAFLTYHGSWNRRIPTGAKVVHVKVQNGKPVSIEDFVTG
                     WQESDGSRWGRPVAVLAMPDGALLISDDTGGRIWRVTYGK"
BASE COUNT          840 a         1861 c         1895 g          776 t
ORIGIN      
        1 ctgcccgacg tcgcgaccgt ggtcgtcccg gtcggcggcg gcgggctcat ctccggtgtg
       61 gcgcgcggcc tcgcggccgc ggggagcccg gcgcgggtcg tcggggtcga gcccgccggc
      121 gcgcccaagc tcacccgctc gctcgaggcc ggccggcccg tcacgctcga gcgcacggcc
      181 agcatcgccg atggcctcat cacgctctcg gtcggcgagc tccccttcga ggtgctgcag
      241 cagcacaagg agcggttcgc gggggtcgtg accgtgaccg acgacgccat ccgggaggcg
      301 gtgttcttcc tgcaccagcg gtgcgggctg atggtggagc cctcgggggc cgctaccacc
      361 gccgcgttgc gggccgggct gctcgagcag taccgtcgcc cgccgatggt gctcgtggtg
      421 agcgggggca acgtggaccc cgcgttgctc aaggaccgcc gccgctcgtg acggcgtctt
      481 ccccgacgtc tccgccgccc accgccgcgg ggaccaagat cctgctcgcc gacgacgacg
      541 agtcgctggt ccaaacgctc tcctacatcc tcaagaagag cggctacgag gtcgtcacaa
      601 gcctcggggg cgagaacctg ctgtcgctcg tcgccgccga acggccccag ctgctgctgc
      661 tcgacatcat gatgcccaag gtggacgggc tggcgctgct gggtctcctc aaggaagacg
      721 accggttccg cgacctgccg gtgctcatgc tgtcgtccat ggatccggag gacgcggccg
      781 tgaggtcgct cggcctgggc gcctcggact tcatcgccaa gccggtgcgc gcgcgcgagc
      841 tgctggcgcg cgtcgaagcg cacctccggc tcggcaaggc ggtcgccgag gcgcgggggg
      901 aggcgaagaa ccgcgccgcc atggtcgaga tcctgcacga gatcacggat tcgctcaaac
      961 ccgaggagat ctaccacatt ctcgtgcggc gcgtggcccg ggtgctcagc atctcgaagt
     1021 gctcgatcgt gatcgccaag gcgggagaca agatgggaac ggtcgtcgcc tcgttcgaga
     1081 acccgatgct gcacaacctc gagatcgaga tggcgcgcta tccggagatc gagcgcgcgc
     1141 tggcgagcag cagctcggtg ctggtctcgg acgtcgccac cgacccgatg tacgcgcggg
     1201 agcgcacccg ctggcaggcg gagcgcatcc tcgtgcccac ccgctcggcg gtcgcgatcc
     1261 cgttcaacat gcgggaccgg caggtcgggg tgttcttcct gcgcaccacc gaggcggacg
     1321 ccgcgctgac gcccgcggac gcgcgctttg cggagtcggt catcgaggcc gcggtggccg
     1381 cgatcgagaa ggcgtacgac ttcgaaacgg cggtgctcga caggcgtcgc ttcgagcagc
     1441 tcgcgtccac cgacgcgctc accggcacga tgaatcgccg cgcgctcagc gaggccctcg
     1501 ccgccgagct ggaccgcgcc cgccgctaca acctctacct cagtatcctg ctggccgacc
     1561 tcgaccggtt caagcaaatc aacgaccggc gggggcatct ggcgggcgac gccgtgttgc
     1621 ggcaagttgg tgagctgctg cgccatgagg cccggtcggt ggacctcgtt gcgcgctacg
     1681 gcggcgagga gttcgtcgtc gtgatgcccg aaaccacgct gcgcggggcc ggtgtattcg
     1741 ccgagcgcgt ccgcggcaag gtcgccgtgc gcgacttcgc cccggcagga gaggagccgt
     1801 tgcagttgac ggtcagtatc gggctcgcgg gcttccccaa cgaccgggtc accagcgccg
     1861 acagcttcat cgcgctggcc gaccaggccc tctaccgcgc caagaacgaa ggccgcaatc
     1921 gggtacgtga atgagtcagc gccgctgtcc caaatgcaag accgtatact ccgactccgc
     1981 ccgcttctgc cccagggacg gctcgatgct ggtggacgtg cagggggccc ccgcgacccc
     2041 catccccacc acgcccaccc cgcagggccc gccgctgggg actccgcgga cgccggccgg
     2101 cgcggccacg gccgtgcgca cgccgcccat caagccgccg cccgccctgg atcgcgcgtc
     2161 gacactctcc aaccagatgc tcgacgcacg ctatcaggtg atgaagaagc tcggcgaggg
     2221 cggcatgtcc tacgtctacc tcgccaagga aatctcgacc ggccgggaag tggccatcaa
     2281 ggtgctctcc cccaagctcg cgagcgacaa gagctcggtc gagcggctgc ggcgggaagc
     2341 gggcctggcg atgcgcctcg accaccccaa cgtctgccgc atcctccggc tgggggaatc
     2401 cgaggacggt ctgatctacc tggtcatgcc cttcctccac ggcgagctgc tctccgaccg
     2461 cgaggtccgg gacggcccga tggacctggc ccacggcctg tccattctgc gccaggtgtg
     2521 cgccggcctg gatcacgccc attcgttgca gatcgtgcac cgcgatctga agcccgagaa
     2581 catcatgctc gtgcccaacg aggacggctc ggaccacgcg gtggtgatgg acttcgggct
     2641 ggccaaggag cggcgcgccg acccctcgat cgccaaactc accgccacgg gcatcatcct
     2701 cggcacgccg tagttcatga gcccggagca gatccgtggc aaggcgctcg atgcccgctc
     2761 cgacgtgtac gccctgggca tcgtggcgtt cgagatgttc accggcaagc tgccgttcca
     2821 ggggcgtaac gcccaggaga tgatgatcgc gcggctgcgc ggcagcccga cgccgatccg
     2881 caccctgcgg cccgagctcc ccgtccccct cgagaaggcg atcgccaagg cgctcgagac
     2941 caatcccgac aaccgctttc ccacgaccct cgccttcgcc gaagcgctga gcgaggcgga
     3001 gacgggcggg ggcggcgcgg cgggcggcgg aggtgggggt gggggtggac tgttcggcaa
     3061 gctgaaggag aagctgagat aaccggtcgc catgaagcgc tttcccgcct tcgatccgcc
     3121 cgaatacctc tcctggcagc ccgaccccgc tctcgttcgc gcgttccgac agacgatcga
     3181 gcagcagccg gagcgcgccg cgctggtcgc gcgcctcgac cgcgacggcc ggcgcgccct
     3241 ctatgcgggg ctggtgcgca cccggctcca cgacgtcacc ctcaaacgct gggtgcgcac
     3301 cggcgtcatc tcgaaggcgt ggctcggcac gggtgaggaa gcggcgaccg tcgggccggt
     3361 gcatgcgctg gagcgtgacc gcgatatcgt cgcgcccatg atccgcaacg ccggggcggt
     3421 gatcgagatg ggcatgccca tcgcccaggc gttgcgtgcc tacctcgcga ccgccgactc
     3481 gccttccggc gggcgcgatc tgcacctggg cgatctggcg gccggcgtgc tgcagccgat
     3541 ctcgcacgtg ggcgacgtgg ttcccgtggt cgcagggatc gccctgacct tcaagatgaa
     3601 acgcgagccc cgcgtggcgc tcacctgggt gggcgacggg tccaccaaga ccgccgcggc
     3661 gcacgagggt accaacttcg cggccgtgca gcggctgccc gcgatcttca tcgtgcagaa
     3721 caaccaggtg gcgctgggga cccgactcga gcagcaccac ctcccgggga cgttcaagga
     3781 ctgggcggcc tcctacggca tgtgggggct cgagttcgac ggcaataacg tgctcgatgc
     3841 ctgggcggcg acgaagctgg cggccgagcg ctgccgcgcg gggcagggac cggcgttgct
     3901 cgtggccgag acgttccgga tgggcggcca tgcgacccac gatgagcgcg aggcccgcga
     3961 gacgtttccc gcgaagctgt tcgccgagtg ggggaagcgg gatccgatcg ggctgtacga
     4021 ggaatatctg aaggaagacg gctggaccga ccgggagctg ggcgacgtgg aggccgaggt
     4081 caccgcggcg gtcgagcgtg cggagcagga ggcgctggcc agccggggga acatgccgac
     4141 cggcgaaggc gcattgtcgg ggctgttcgc gacgcagggg tgagcagcag gatcctcgag
     4201 gaggttcgta tgcagcgacg gatggtcatg gcggtgttgt tcgggatcgc ctgcacgacg
     4261 gggggaagcg tggcggggca ggccccggcc ggaatggtgg ctcaggagat cgcggggcac
     4321 acgttgtacc tgcggcccgg cttcaagatc gggatattcg ccgacaacca gcgcggcgtg
     4381 cgcaacatcg tgctcggccc acacggggcc gtgtacgcgg cgatgcaggg ggccggcagg
     4441 gtgctgcggc ttgtggacgc aaatggcgat ggcgtggccg attcggtgac tcctgcggcg
     4501 accggactca acggaccctt cggcatcgcc ttccgcggcg acacgatgta cgtcggcgaa
     4561 acgagccaga tcacgcgcat cgcccccggc ggcgcgccgc ggccgatcgt caccggactg
     4621 ccgacggggg gacattccac ccgcaccgtg gtgttcgggc cggacggcta cctctacctc
     4681 gcggtcggct cttcgtgcaa catctgcgat gagaccgatc cgcgccgcgc cgcggtgacc
     4741 cgcttcaacg ccgacggctc gggcgagcac cgcttcgcca ccgggttgcg caactccgtc
     4801 ggcctcgcct tctcaccgac gaccggcgag ctctggggca ccaacaacga ccgcgacaac
     4861 atcgggggtt cgaacacctc gatgaccgac gacctgcccc cggagcacct gaacatcatc
     4921 aaggacggca agttctacgg ctggccgcag tgctaccttc cggggcagca caaccccgag
     4981 tatcccagcg cgagctgcgc caacgtcgag ccgccggcca tcaccttcca ggcccactcg
     5041 gcgccgctgg ggatcacgtt ctatacgggc tccgccttcc ccggctacac cggcgacgcg
     5101 ttcctgacct accatggctc gtggaaccgc cgcatcccca ccggcgcgaa ggtcgtgcac
     5161 gtgaaggtac agaacgggaa accggtctcc atcgaggact tcgtcacggg gtggcaagag
     5221 agcgacgggt cccgctgggg acggcccgtg gccgtgctgg cgatgccgga cggggcgctg
     5281 ctcatctccg acgataccgg cggccggatc tggcgggtga cgtacgggaa gtgaggggga
     5341 ggacggtgga ggacggtgga ggagggttac gg
//
LOCUS       DBNGZW010000111         4213 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1169973, whole genome shotgun sequence.
ACCESSION   DBNGZW010000111 DBNGZW010000000
VERSION     DBNGZW010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4213)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4213)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4213
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1169973"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..372
                     /locus_tag="AC16JC_02715"
     CDS             <1..372
                     /locus_tag="AC16JC_02715"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5163638.1"
                     /translation="ARRTGIPCTVAAPASWPQIDEARSTALFRVLQEALTNVARHAQA
                     SSVRVRVAIEDGWLVLAVEDDGKGVANRQSTGPKSLGLVGMRERVAAFGGELRLASRE
                     PSGTILEARMPARPSVEWRQV"
     gene            369..998
                     /locus_tag="AC16JC_02720"
     CDS             369..998
                     /locus_tag="AC16JC_02720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014260863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5163639.1"
                     /translation="MIRLLIADDHPVVREGLRRVVESAAFQVVGEATTGDELLARAAQ
                     VEADVVLLDITMPGPGFVEIIERLRRERPRLRVLVLSVQPEDQFGLRVLQAGAAGYLS
                     KNQSPEQLVAAIRRVVSGGRYVSPALAEKLASVVSKGDNRRPHELLSAREYQVLCLIG
                     AGKSVKEVAAAMSLSPKTVSTYRTRVLEKTGLKSNAEIIRYTVENSLTS"
     gene            1159..1758
                     /locus_tag="AC16JC_02725"
     CDS             1159..1758
                     /locus_tag="AC16JC_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163640.1"
                     /translation="MSRLQSSLFPLLLVSVALGCTDRSMAPTADVDSHLPALGLSRAS
                     ADGREGFGFNGTASGFPTGAVFLTGGGAYDPATASNVIPTDTTFAHSGGGFRCTAAVA
                     QGPLSGCAEGEGVRWDTAQLLASTSFKCTATDAPKPAATGDKTVVLLADFYRAGDGID
                     ESFTAQMIVSENDLADNIPGIQNLWVQGVGCGTAVVNFN"
     gene            1835..2197
                     /locus_tag="AC16JC_02730"
     CDS             1835..2197
                     /locus_tag="AC16JC_02730"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5163641.1"
                     /translation="MSSIDRPLSGAALRFSLAEERARVHDADLLTRNGRNARTLVKEG
                     RLRVTLVMVAAGGRIAPHQTEGPITVHVLDGALRFRAAGKEHALSAGDLLVVDAGVEH
                     AVESESGGTFLLTVVQSG"
     gene            complement(2208..3494)
                     /locus_tag="AC16JC_02735"
     CDS             complement(2208..3494)
                     /locus_tag="AC16JC_02735"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR5163642.1"
                     /translation="MHRLIVALAIALLAAARAPLTAQSRASTLVAAARTQLSASRLDS
                     ADVLLRAALDSATQRADSVSVLVWRAVLWFLRGDESRTATAFYQALTLDSTLNVQGLD
                     RISPLLESLLQEERLAVAGTPPTYLSDLIDDRPQRLSGPPVRYPPDLLRRQVRGRALV
                     GLTVDTMGHAEPASIQILATPDSGFIEPLRRMLLASTFSPGRVRGRAVRTMIELAIDL
                     VPGTPPNATTLVTAARAQLAAHRTDSAFALLRDALDPDTRTTEGERVYALLVRGNAWR
                     AVGRDSLARTDYDSALAGYRRLTAQGVELAPVLKRLADSVRLVRRSPAATDPLGAPVA
                     GSAVDVQPALLSHPKIRYPPEMQSLRVGGTVTVEATVDTTGRVVPGSLKVSQSPNPGL
                     DAEAMRVVAGSHYKAARRGGHAVTTVIRQAITFTPY"
     gene            3681..>4213
                     /locus_tag="AC16JC_02740"
     CDS             3681..>4213
                     /locus_tag="AC16JC_02740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HIR5163643.1"
                     /translation="MDRAILYVNPLTFPIAVERVAQRSARFLDRPLAIAPGGTDRAII
                     WSASPEAALAGVTAGMPIYQALRRCPDLLLLPPRYGLYRRAAQALERILQPYVPVIEP
                     HTLGHAYGDLSGTRKLFGPATDVAVRIRREIWGRLGLPVTVGLAASKVVSEVAAHVLK
                     PEPVVDVRPGDEPAFLAP"
BASE COUNT          631 a         1404 c         1502 g          676 t
ORIGIN      
        1 gcccggcgca cggggattcc ctgcaccgtg gcggcgccgg ccagctggcc ccagatcgat
       61 gaggcccgtt ccacggctct cttccgcgtg ctgcaggagg ccctgacgaa cgtcgcccgc
      121 cacgcccagg cgtcgagcgt ccgggtgcgc gtcgccatcg aggatggttg gctcgtgctg
      181 gccgtcgaag acgacgggaa gggggtcgcg aatcgccaaa gcacgggacc gaaatcgctg
      241 gggctcgtcg gcatgcgcga gcgggtggcg gcattcggtg gtgaactgcg gttggcctcg
      301 cgtgagccgt cgggcacgat cctcgaagcc cgcatgccgg cccgtccctc ggtcgaatgg
      361 cggcaggtgt gatccggttg ctcatcgccg acgaccaccc cgtggtgcgc gagggcctgc
      421 gccgggtggt ggagtccgcg gccttccagg tcgtgggcga ggcgacgacc ggcgacgagc
      481 tgctggcgcg cgccgcccag gtcgaggcgg atgtcgtgct gctcgacatc accatgcccg
      541 gtccggggtt cgtcgagatc atcgaacgcc tgcgccgcga gcgtccgagg ctgcgcgtgc
      601 tggtgctgag cgtacagccc gaggatcagt tcggcctgcg ggtcctccag gcgggagcgg
      661 ccggctacct gagcaagaac cagtcgcccg agcagctcgt cgccgccatc cggcgcgtcg
      721 tgagcggagg gcgctacgtc agcccggcac tggccgagaa actcgcgtcg gtggtctcca
      781 agggcgataa tcgccgaccg cacgagctgc tgtcggcgcg ggagtatcag gtcttgtgcc
      841 tgatcggcgc cggcaagagt gtcaaggagg tcgccgccgc gatgtcgctc tcacccaaga
      901 cggtgagcac ctatcgcacg cgcgtgctgg aaaagaccgg tctcaagtcg aacgccgaga
      961 tcatccgcta caccgtcgag aattcgctga cgtcctgagg cgggtgtagg acgcccaccg
     1021 acacggtgtc ggcaacttcc cggccggcat gcgcgcgtgc ggccgattct gcgtccccac
     1081 ccccatcact atcctgagcc ttccgccgcg gcaccagcgc ggcgaccggc cgtcaccacg
     1141 cacagctcag gaggtcccgt gtctcgactg caatcttcgc ttttcccgct gctgctcgtc
     1201 tcggttgcac tggggtgtac cgaccgctcg atggcgccca cggcggacgt ggactcgcac
     1261 ctgcccgcgc tcggtctctc ccgggcgtcc gccgacggtc gcgaggggtt cgggttcaac
     1321 ggcacggcga gtgggttccc gaccggcgcc gtgttcctta ccggcggcgg ggcttacgac
     1381 ccggccacgg ccagtaatgt gattccgacg gatacaacgt tcgcgcattc aggtggcggg
     1441 ttccgctgca ccgcggccgt ggcgcagggc ccactctcgg gatgcgccga gggcgagggc
     1501 gtgcgatggg acacggctca gctcctggcc tccacgagct tcaagtgcac tgccaccgat
     1561 gctccgaagc cggcggcaac gggcgacaag acggtcgttt tgctggccga cttctaccgt
     1621 gccggcgacg gtatcgacga gtcgttcacc gcgcagatga tcgtgtcgga gaacgatctc
     1681 gcggacaaca tccccgggat tcagaacctc tgggtgcagg gcgtcggctg cgggaccgcc
     1741 gtggtcaatt tcaactgacc tgaaggcggg atggctcact cagcgggcgc ggcgaccact
     1801 ggcgcgcccg ccattttctc ttgacattcc cgtcatgtca tcgatcgatc gcccgttatc
     1861 gggcgcggca ttgcgtttct cactggccga agagcgggcg cgggtccatg acgcggatct
     1921 gctgacgcgc aacggccgca acgcgcgcac cctcgtgaag gaaggacggc tgcgcgtcac
     1981 gctcgtcatg gttgccgcgg gcggacgcat cgcgccgcac cagacggaag gtccgattac
     2041 ggttcatgtg ctggatggag cgctccggtt ccgggcggcc gggaaggaac acgcgctgag
     2101 cgccggcgac ctcctggtcg tggatgccgg cgtggagcac gcggtggagt cggagagtgg
     2161 gggaacgttc ctgctgacgg tcgtccagtc ggggtaaacg gagcccgcta gtacggcgtg
     2221 aaggtgatcg cctggcggat gaccgtagtg acagcgtgcc cgccgcgccg ggccgctttg
     2281 tagtgcgagc cggccacgac tcgcatcgcc tcggcatcga gcccgggatt tgggctctgc
     2341 gacaccttga gcgagcccgg cacgacgcgt cccgtggtgt ccaccgtcgc ttcaaccgtc
     2401 accgtgcccc cgacccgcag gctctgcatc tccggcgggt aacggatttt gggatgggac
     2461 aacagcgcgg gctggacatc gactgctgag cccgccaccg gagctccaag cggatccgtg
     2521 gcggcggggc tgcggcggac cagtcgcacg gaatcggcga gccgtttgag taccggggcg
     2581 agctccacac cctgggcagt caggcgccgg tatcctgcga gcgccgagtc gtagtcggta
     2641 cgagccagcg aatcccgtcc caccgcacgc caggcatttc cccgcacgag cagggcgtac
     2701 acgcgctcgc cttccgtggt ccgcgtgtcc gggtcgagcg catctcgcag cagggcgaag
     2761 gcgctgtcgg ttctgtgggc ggcgagctgg gcgcgagcgg ccgtcacgag cgtcgtggca
     2821 ttcggaggcg tgcccgggac gaggtcgatg gccagctcga tcatcgtgcg cacggcccgt
     2881 ccgcggacgc ggccggggga gaaggtcgac gcgaggagca tccggcgcag cggttcgata
     2941 aagcccgagt ccggcgtcgc gagaatctgg atggacgccg gttccgcgtg ccccatggtg
     3001 tcgacggtca gaccgaccag cgcgcgcccg cgtacctggc ggcgcagcag gtcgggcgga
     3061 taccggaccg gcggaccgga caggcgctgt ggcctgtcgt cgatcaggtc ggagagatag
     3121 gtgggagggg taccggcgac ggcgaggcgc tcttcctgga gcagggactc gaggagcggc
     3181 gagatgcggt ccagaccctg gacgttcaac gtcgagtcga gcgtcagtgc ctgatagaag
     3241 gcggtggcgg tccgggactc atcgccccgc aggaaccaca ggaccgcccg ccacaccaac
     3301 acggagacgc tgtcggcacg ctgggtggcg ctgtccagcg cggcgcgcaa caggacgtcc
     3361 gcgctgtcca gtcggctggc ggagagctgg gtccgggccg ctgcgacgag cgtgctggcg
     3421 cgactttgcg ccgtcagcgg agctcgggcg gctgcgagca atgcgatggc gagggcgacg
     3481 atgaggcggt gcatggcgat cgaggccccg atataacgcg ccgactcaag tccgccagcg
     3541 ggcggcgtcc tatacgatgc aattattgac actgtcgcgt cattcacaag acattatcta
     3601 gcatttgcaa caataaatcg gcttcttgac tttcggcttt tattcgggca cgttctctct
     3661 cccagagccc ggtgtgcccg atggaccgcg ccattctgta cgtcaatccg ctcacctttc
     3721 cgatcgcggt cgagcgtgtg gcgcagcgga gcgcgcggtt tctcgatcgg ccgctcgcga
     3781 tcgctcccgg ggggacggac cgcgcgatca tctggtcggc gtcgccggaa gcggccctcg
     3841 cgggcgtcac ggcgggcatg cccatctatc aggcgctgcg gcgctgcccc gatctgctgc
     3901 tcctgccgcc gcgctacggt ctctaccggc gcgccgccca ggccctcgag cgcatcctcc
     3961 agccgtacgt gccggtgatc gagccgcata cgctgggtca cgcctacggc gacctctccg
     4021 gcactcgcaa actgttcggc ccggcgaccg atgtcgccgt ccgcatccga cgcgagatct
     4081 gggggcgcct cggcctgccg gtgaccgtgg gcctcgccgc cagcaaggtg gtgagcgaag
     4141 tggcggcgca cgtgctcaag cccgaaccgg tggtggacgt gcggcccggc gacgagccgg
     4201 cgtttctcgc ccc
//
LOCUS       DBNGZW010000112         3044 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1578965, whole genome shotgun sequence.
ACCESSION   DBNGZW010000112 DBNGZW010000000
VERSION     DBNGZW010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3044)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3044)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3044
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1578965"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..881
                     /locus_tag="AC16JC_02745"
     CDS             <1..881
                     /locus_tag="AC16JC_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR5163644.1"
                     /translation="QLGLRYMRRGGYDVRRMPEVFSLLERVSQTSGGGRVPDWLSTHP
                     NPVNRAGAIERAIAALPADSLGTVVNHDAYLRQIDGIVFGDNPRDGFFHGTEFFHPDL
                     KFRFTFPQGWTTQNQRQAVLAVSAQQDAIEQVSMSDKATPEAAAQAFFSQQGVRGSAG
                     RGTIHGFAAAGGAFSALTQDSTPLAGRVAFVSYDGHVYQLLAYGPQARWSAYASAADQ
                     SISSFDRLSDPTLLAVQPWHVQLVNLTRVMSLDEFVQRNPGPATPTATALVNNIDPNG
                     RLGTGLVKRIVGQPLP"
     gene            878..1651
                     /locus_tag="AC16JC_02750"
     CDS             878..1651
                     /locus_tag="AC16JC_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010994651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5163645.1"
                     /translation="MSRIAGKTALVTGASAGIGLACAQRFAAMGAHLVLWARRSDRLD
                     AEAELIRRAHGVEVRTATVDVRDRSAVDRAAAALVATGAVPEVLVNNAGLAAGLDLLH
                     EGDPEDWDRMIDTNIKGLLYVTRAFLPHMVERGRGHVVNLGSLAGHQTYPRGNVYGAT
                     KYAVRALTESMALDLVGTPIRVSSVSPGLVETEFSVVRFAGDAERAKAVYKGMRPLTA
                     DEVAEVIGYVVDLPEHVNVLDVVLMPVAQRSTQIVHRES"
     gene            complement(1661..2224)
                     /gene="efp"
                     /locus_tag="AC16JC_02755"
     CDS             complement(1661..2224)
                     /gene="efp"
                     /locus_tag="AC16JC_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR5163646.1"
                     /translation="MPIAATNIRRGMAIMFEGEPCRVIEFHHHTPGNLRAMVHAKLRK
                     LKTGVTVEHRFRASDSVEEAHLETHELQYMYHDGHSYHFMNVQNYEQYELDEEALGDH
                     AQWMTDGMQVIAEYFEGRPIGVELPNEIVFEIVETAPVMRSATKTASTKPAKLSNGVS
                     VNVPEFIQTGEKVRVNPNTGEYLDRAK"
BASE COUNT          477 a         1009 c         1068 g          490 t
ORIGIN      
        1 accagctcgg cctgcgctac atgcgccgcg gcggctacga cgtccgccgc atgcccgagg
       61 tgttctcact gctcgaacgc gtcagccaga cgagcggcgg cggccgggtg ccggactggc
      121 tctccacaca ccccaacccg gtcaaccggg ccggggcgat cgagcgcgcg atcgccgcgc
      181 tgccagcgga ttcgctcggc accgtcgtga accacgacgc ctatctgcgc cagatcgacg
      241 gcatcgtgtt cggggacaac ccgcgggacg ggttcttcca tggcaccgag ttcttccacc
      301 ccgatctcaa gttccgcttc acgtttcccc agggatggac gacgcagaac cagcggcagg
      361 ccgtcctggc ggtgagcgcc cagcaggacg ccatcgagca ggtgtcgatg agcgacaagg
      421 cgacgcccga ggccgcggcc caggcgttct tctcgcagca gggcgtccgg ggctcggccg
      481 gacgcggcac gattcacggc ttcgcggcgg cgggtggcgc gttcagcgcc ctcacccagg
      541 acagcacgcc cctggccggc cgggtcgcct tcgtgtcgta cgacgggcac gtgtaccagc
      601 tgctcgccta cggcccccag gcccgctggt ccgcctatgc ctccgcggcc gaccagtcca
      661 tctccagttt cgaccgcttg agcgacccca cgctcctggc cgtgcagccg tggcacgtgc
      721 agctcgtcaa cctgacgcgg gtgatgtcgc tcgacgagtt cgtgcagcgc aacccggggc
      781 cggccacgcc taccgcgacc gccctggtga acaacatcga tccgaacggc cggctgggga
      841 ccggtctcgt caaacgcatc gtggggcaac cgctgccgtg agtcgcatcg ccgggaagac
      901 ggcgctggtc acgggcgcca gcgccgggat cggcctcgcc tgcgcgcagc ggttcgcggc
      961 gatgggcgcc cacctcgtgc tgtgggcgcg ccgcagcgac cggctcgacg ccgaagcgga
     1021 gctgatccgc cgcgcgcacg gcgtcgaggt gcggaccgcc acggtcgacg tgcgcgaccg
     1081 ctccgccgtg gaccgggcgg ccgccgcgct cgtggcaacg ggcgccgtcc ccgaggtcct
     1141 cgtcaacaac gccggcctcg cggccgggct cgacctgctc cacgagggcg accccgagga
     1201 ctgggaccgg atgatcgaca cgaacatcaa ggggttgttg tacgtgaccc gcgcgttcct
     1261 gccgcacatg gtcgagcgcg ggcggggaca cgtcgtgaac ctggggagtc tcgccggcca
     1321 tcagacctat cccaggggca acgtgtacgg ggcgacgaag tacgcggtgc gggccctcac
     1381 cgagagcatg gcgctggatc ttgtcggcac gccgatccgc gtgtcgagcg tttcccccgg
     1441 gctggtggaa acggagttct cggtcgtgcg cttcgccggc gatgcggagc gggcgaaggc
     1501 ggtctacaag ggaatgcggc cgctcacggc ggacgaggtg gccgaggtga tcggctacgt
     1561 ggtggacctg cccgagcacg tcaatgtgct cgatgtggtg ctgatgccgg tggcccagcg
     1621 cagcacccag atcgtccacc gggagagttg agccggcggg ctatttcgcc cggtcgagat
     1681 actcccccgt gttcggattc acgcgcactt tctcgcccgt ctggatgaac tcggggacgt
     1741 tcaccgacac tccgttggag agcttcgcgg gcttggtgga cgccgtcttg gtggcgctgc
     1801 gcatcaccgg cgcggtctcc acgatctcga acacgatctc gttcggcagc tcgaccccga
     1861 tcggccggcc ttcgaagtac tcggcgatga cctgcatgcc gtcggtcatc cactgggcgt
     1921 ggtcgccgag cgcttcctcg tcgagctcgt attgctcgta attctgcacg ttcatgaagt
     1981 ggtacgaatg cccgtcgtgg tacatgtact gcagctcgtg cgtctcgagg tgggcttcct
     2041 ccaccgagtc ggaggcgcgg aagcggtgtt cgacggtcac gccggtcttc agcttgcgca
     2101 gcttggcgtg caccatggcg cgcaggttgc ccggcgtatg gtgatggaat tcgatgacgc
     2161 ggcagggctc gccctcgaac atgatggcca tgccacgacg gatgttggtt gcggcgatcg
     2221 gcatacgcgg tcctgtggga tggagaatga gccggtgcag ccgccccctc gggcggcgcg
     2281 cgacgggcgg ggaatctaag cggagccccg ggcccgcggc acccggggaa ccccgcgcgg
     2341 ccacggcctc gtgacgacct gacccgggcc ggtgccggca aggtgcgctc ggccagatcc
     2401 cggaacaatt ggcgaccgcg gcaacgttct tcatgatgtc aggcgaggcg acagggcggg
     2461 accgggcgac cgttcatcgc caccgaacga agcgcacgcc cggtgggact tgacagtgat
     2521 gggtggcgca gtagaatgcg ccaagggagt cgcggcggcc ttcacctgag cctgtgtggc
     2581 ggaggggtta gaacggtcgg accagcgact cttttcgttt gtggtgtatg agcgacgtgg
     2641 agtgtcttgg gaagatgggg cagatcgaac tgacaagcac gagtcccgac gctttccccg
     2701 cccggcataa ccgcagggcc gggagggtgg agcatcctct ggcacgccat atgccggagc
     2761 gtggtacgac ggacgccggc acaggcaggg aaggcgcgac cacgtcgtag gcgcgctcgt
     2821 tcccccgtga gcgacttcat cgccccgcct gtgacgacga cagacggggc gatcgatttt
     2881 ttcagcagta tggagggacg gtaaacctgc cacccggagg aaaccatgga acgttcccgc
     2941 agtgagttcc gactgggtga cttcttcgtg gtgtcgaccg atggggacac ctggtgcgtg
     3001 agcaccgaaa tggcgcgcca catcgacagc tgtctcgatg ccga
//
LOCUS       DBNGZW010000113         3134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_622928, whole genome shotgun sequence.
ACCESSION   DBNGZW010000113 DBNGZW010000000
VERSION     DBNGZW010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_622928"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            145..1203
                     /locus_tag="AC16JC_02760"
     CDS             145..1203
                     /locus_tag="AC16JC_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012241871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5163647.1"
                     /translation="MASVTLLGLTKRFSSGAPALQDLTLEVADGEFLVLLGPSGCGKT
                     TALRCIAGLEEPTAGEIRIGPRDVTHLPPGDRDVAMVFQNYALYPHLTVRQNIAFPLE
                     MRRTPEAEVVRRVRQAADRLGLGDLLERRPGELSGGQRQRVALGRAIVRQPSVFLFDE
                     PLSNLDARLRVEMRAELLTLHRDLAATMIFVTHDQIEAMTMGQRIAVLHEGRLRQIGA
                     PADVYRAPTDAFVAAFVGSPGMNILAGAPEPERKRVVISGSLRFRMPANVFGPVQIGI
                     RPEHLSLGVEGEGVGDATVRMIEPLGSDTLIHLDAGGTRLVAKVPGIPLLAAGMGVGV
                     QVEPANVHLFDAGGARIG"
     gene            1200..2489
                     /locus_tag="AC16JC_02765"
     CDS             1200..2489
                     /locus_tag="AC16JC_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5163648.1"
                     /translation="MKYAFVFAAALAVDACGAAAPAGGRTELELANWADYREADLENR
                     VLAPFEWSHPGITVQQQSAGTGQAEYRERILTSIAAGHPPDVLLLDNIDVPAFTNRGV
                     LLDLAPYLPRLGIDLARYDPTVLDVFRRGSAVYALPKGYTPMVIMYNKDLFDRAGIPY
                     PTGDWKWDDFLRIAHALTRDTDGDGQVDQWGTAYDRRYFLWAAWLWSGGGDILCPGGW
                     RATGCLDSPASIEAIRWYTDWVTRDSIVPRAHNLRRSMGDNLRLFYSGKVAMLTAGHF
                     WIPNVRPYTADGRLRVGFAEIPHRTGAAPQTVIYASGLAVPATALHRKLSVQLAAYLA
                     DSLAQSIRAEAGLELPSLTAAAQALAIADTTEWEAVFLRASKHARIPWGARIEPWREV
                     EAALPDMLDRITLEHVDPAVAAHEMALRLDRLLAQDQ"
     gene            2486..>3134
                     /locus_tag="AC16JC_02770"
     CDS             2486..>3134
                     /locus_tag="AC16JC_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163649.1"
                     /translation="MKLGWRLAAALVAVAGGLTALGVGRAAARKAEWEAAARTARTAA
                     AYLAIVTPAPSPTGGASYDLVRLLVQARGLGRLPGWRGQVEVYHGTAPLVRATDPPLS
                     TALFTELREREATRRLDGVMVAPLKDRDDWDVVGAVAVRPAAPEPLLAGWNLVLLVVL
                     ALAGMLALDGGRRAALVCGAVAVLVGLVAQREAVQGRNDATARWMSQTGLLLEEAA"
BASE COUNT          438 a         1081 c         1148 g          467 t
ORIGIN      
        1 acggatctcc gagagcatgg gatgccggca tgcggccgtg acgctcgagc tggccgatga
       61 gcacgaggtc gagttctggc acgcgaagga gtgaggccgg cagggtttcc tccgtagcag
      121 tttaacccta ggttaccccg cgcgatggcc tccgtcaccc tcctcgggct caccaagcgg
      181 ttcagctccg gcgcaccagc gctgcaggac ctcacgctcg aggtggcgga cggggagttt
      241 ctcgtgctgc tggggccctc gggatgcggc aagaccacgg cgttgcgctg catcgcgggt
      301 ctcgaggagc ccacggcggg agagatccgc atcggcccgc gggacgtgac gcacctgccg
      361 ccgggcgatc gcgatgtcgc catggtcttc cagaactacg cgctctatcc gcacctcacc
      421 gtgcggcaga acattgcctt cccgctcgag atgcgccgca caccggaagc ggaggtcgtg
      481 cggcgcgtgc ggcaggcggc cgaccggctg ggtctgggcg acctgctgga gcggcgcccg
      541 ggagagctgt cgggggggca gcgccagcgg gtggcgctgg gacgcgccat cgtgcgccag
      601 ccgagcgtgt tcctgttcga cgagccgctc tccaatctcg acgcccggct gcgcgtcgag
      661 atgcgggcgg agctcctgac tctgcaccgc gacctggccg cgacgatgat cttcgtcacg
      721 cacgatcaga tcgaggcgat gaccatggga cagcgcatcg ccgtgctgca cgaggggcgg
      781 ctgcggcaga tcggcgcgcc cgccgacgtg taccgtgccc cgaccgacgc cttcgtcgcc
      841 gcgttcgtcg gcagccccgg catgaacatc ctggccggcg cgccggagcc agaacgcaag
      901 cgggtcgtga tcagcgggtc gctccggttc cgcatgccgg cgaacgtctt cgggcccgtc
      961 cagatcggga tcaggcccga gcacttgagc ctcggtgtcg agggggaggg cgtgggagac
     1021 gcaaccgtcc gcatgatcga gcccctgggg agcgacacgc tgattcacct cgatgccggc
     1081 ggcacgcgcc tcgtggcgaa ggtgccgggg attccgctcc tcgccgcggg catgggagtg
     1141 ggcgtgcagg tggagccggc caacgttcat ctcttcgatg ccggcggggc gcgcatcgga
     1201 tgaagtatgc gttcgtcttc gccgccgccc tggccgtgga tgcctgtggg gccgctgccc
     1261 cggcgggcgg gcgcaccgag ctggagctcg ccaactgggc cgactaccgg gaagcggatc
     1321 tcgagaaccg ggtactcgcc ccgttcgagt ggagccaccc cgggatcacg gtccagcagc
     1381 agagcgccgg cacggggcag gccgagtatc gcgagcgcat cctcacgtcg atcgcggcag
     1441 gccatccccc ggatgtcctt ctgctcgaca acatcgacgt cccggcgttc acgaaccgcg
     1501 gggtactgct cgatctcgca ccctacctcc cgcgcctggg catcgacctc gcgcgctacg
     1561 atccgaccgt gctcgacgtg ttccgccgcg gctccgcggt gtacgcgctg cccaaggggt
     1621 acacgccgat ggtcatcatg tacaacaagg acctgttcga ccgcgccggc atcccgtatc
     1681 cgaccggcga ctggaagtgg gacgacttcc tgcggatcgc gcacgcgctg acgcgcgaca
     1741 ccgacggcga cggacaggtg gaccagtggg gcaccgcgta tgaccggcgc tacttcctgt
     1801 gggccgcgtg gctgtggtcc gggggcggtg acatcctgtg ccccggcggc tggcgcgcga
     1861 cgggctgcct cgactcgccg gcgagcatcg aagcgatccg ctggtatacc gattgggtga
     1921 cccgcgacag tatcgtgccg cgcgcgcaca atctgcgtcg ctcgatgggg gacaacctgc
     1981 gcctgttcta ttcgggcaag gtcgcgatgc tgacggcggg gcacttctgg atccccaacg
     2041 tccggccgta tacggccgac ggacgcctgc gcgtgggatt cgcggaaatc ccgcaccgaa
     2101 caggcgcggc tccgcagacc gtgatctacg cgtcggggct cgccgttccc gcgacggcac
     2161 tgcatcgcaa actttcggtt cagctggccg cgtacctggc ggattcgctc gcccagtcga
     2221 tacgggccga agcaggcctc gagctgccca gtctcacggc cgcggcccag gcgctcgcga
     2281 tcgccgacac gacggagtgg gaagcggtct ttctgagggc cagcaagcac gcccgcatcc
     2341 cgtggggcgc gcgcatcgag ccctggcgtg aggtcgaggc ggcgctcccc gatatgctgg
     2401 atcgcatcac gctcgagcac gtggatccgg ccgtcgcggc gcacgagatg gcgctgcgac
     2461 tcgaccgcct gctggcgcag gaccagtgaa gctggggtgg cgtctcgcgg cggcgctggt
     2521 cgcggtcgcc ggcggcctga cggcactcgg cgtgggtcgc gcggccgccc ggaaggccga
     2581 gtgggaagcg gcggcccgga cggcccggac cgcggccgcg tatctcgcga tcgtgacgcc
     2641 cgccccgagc ccgaccggcg gcgcgagcta cgatctcgtg cggctgctgg tgcaggcgcg
     2701 tggactcggc cggctgccgg gatggcgcgg acaggtcgag gtgtatcacg ggaccgcgcc
     2761 gctggtgcgg gcgaccgatc cgccgttgtc caccgcgctg ttcaccgagc tgcgcgaacg
     2821 ggaagcgacc cggcgcctgg acggcgtcat ggtggccccg ttgaaagacc gggacgactg
     2881 ggacgtggtc ggcgccgtgg ccgtccgccc ggcggcgcct gagccgctgc tcgcgggctg
     2941 gaacctcgtg ctgctcgtcg tcctcgcgct cgccggcatg ctggcgctgg acggcggccg
     3001 tcgagccgcg ctcgtctgcg gtgccgtcgc ggtcctggtc ggactggtcg cccagcggga
     3061 agcggtgcag gggcgcaacg acgcgaccgc ccgctggatg agccagaccg gcctgctcct
     3121 cgaagaggcg gccg
//
LOCUS       DBNGZW010000114         2491 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_622964, whole genome shotgun sequence.
ACCESSION   DBNGZW010000114 DBNGZW010000000
VERSION     DBNGZW010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_622964"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="AC16JC_02775"
     CDS             <1..593
                     /locus_tag="AC16JC_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5163650.1"
                     /translation="VDLVADGAEGLRLASDGHYDVVVLDLRLPSMDGRDVLRTLRDRG
                     NAVPVLVLTAQDAVDFKVQALRMGADDYVTKPFALEELLARVEALGRRPKALAPPLLR
                     VGDLVLDSGSREVRRGGRPIELTPKEYAVLEYLVRHRGRVMPRTLITEYAWDYHFDPG
                     TNIVDVVITRLRKKIDQPREPKLIHTVRGVGYVVKE"
     gene            595..2067
                     /locus_tag="AC16JC_02780"
     CDS             595..2067
                     /locus_tag="AC16JC_02780"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5163651.1"
                     /translation="MGTLRGRLTAWYATALALALTGFTVALYLTRRHASYQELDRRIA
                     SEADLTAGILAGVSRAGGVVVTRDPNGRPTLVSELQATLDAVPDFLMITDSLGQPVYT
                     STNTNLIYEQFEQLRRLILAARGRRVQGTIRLIAGGPTFRYAVHPLVDVAPVGGAVLA
                     GARPESVELGLKPLLSTFLFTLPLGILAAYVLGWWLAFRAVQPVDRLTTEVREITDGR
                     TLHRRVPVPFVKDEVGRLAETLNQMLGRLERSFTALRRFTADASHELKTPLTVLRAGV
                     ERAITTPGMPQEALADLEETLQEINRMTELVDTLLTLARADEGTAPLHLEPVDLRALL
                     LDTEETAELLAEPAGVTVSVEAVEGPVVVQADPSRFRQLVMNLLSNAVKYTPQGGRVR
                     LWLERDDSRVRFGVSDTGIGIAPGDLPHIFHRFYRADTARTRTVDRPGVGLGLAICKW
                     IAEAHGGTIDVQSRPGRGTTFTVTLPAAPKPASDASPAKS"
     gene            2064..2276
                     /locus_tag="AC16JC_02785"
     CDS             2064..2276
                     /locus_tag="AC16JC_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163652.1"
                     /translation="MRCPALVILCCILPLRLLAQHDAGDALVLLAIVRYHQGDGAAGV
                     AAFRAACSPRCEGLDVAPIEMRPKTH"
BASE COUNT          358 a          886 c          860 g          387 t
ORIGIN      
        1 aggtggatct cgtggctgac ggcgcggaag gactccgcct cgcctccgac ggccattacg
       61 acgtcgtggt cctcgacttg cgcctgccgt ccatggatgg acgggacgtg ctgcggaccc
      121 tgcgcgaccg cggcaacgcc gtcccggtgc tggtgctcac ggcccaggac gcggtcgact
      181 tcaaggtcca ggcgctgcgg atgggggccg acgactacgt gaccaagccg ttcgcgctgg
      241 aggagctgct ggcccgggtg gaggcgctgg ggcgccggcc caaggcgctc gccccgccgc
      301 tgttgcgggt gggcgatctg gtgctcgact cgggcagccg ggaggtgcgg cgcggcggcc
      361 ggccgatcga gctgacgccc aaggaatacg ccgtgctgga atacctggtg cggcatcgcg
      421 ggcgcgtcat gccgcgcacg ctgatcaccg aatacgcctg ggactaccat ttcgaccccg
      481 ggacgaacat cgtggacgtc gtgatcacgc ggctgcgcaa gaagatcgat caaccacgcg
      541 aacccaaact gatccacacc gtccgcggtg tcggctacgt ggtgaaggag tgaggtgggg
      601 accctccgcg gccggctgac ggcctggtac gcgaccgccc tcgcgctcgc cctcaccggt
      661 ttcaccgttg cgctgtacct cacccgccgc cacgcgtctt accaggagct cgaccgccgc
      721 atcgcgtccg aagcggatct gacggcgggc atcctggccg gcgtgtcgcg cgccggcggt
      781 gtggtcgtga cgcgcgaccc caacggccgc ccgacgctcg tctccgagct gcaggcgacg
      841 ctcgacgccg tgcccgactt cctgatgatc accgattccc tcgggcagcc ggtgtacacc
      901 tcgacgaata cgaacctcat ctacgagcag ttcgagcagc tgcgccggct catcctcgcg
      961 gcgcgcggcc ggcgcgtgca ggggaccatc cggctgatcg ccggggggcc gacgttccgc
     1021 tatgccgtgc accccctggt ggacgtggct cccgtgggcg gtgccgtcct ggcgggggcc
     1081 cgtcccgaga gcgtggagct ggggctcaag ccgctgctct cgaccttcct cttcacgctg
     1141 ccgctcggaa tcctcgccgc ctacgtcctc ggctggtggc tcgcgtttcg cgcggtccag
     1201 cccgtggacc ggctcaccac cgaggtgcgc gagatcaccg acgggcgcac gctgcatcgc
     1261 cgggtccccg tgcccttcgt gaaggacgag gtcggacggc tcgccgagac gctcaaccag
     1321 atgctgggcc gcctggagcg aagtttcacg gcgttgcgtc gtttcaccgc cgatgcgagc
     1381 cacgagctga agacgcccct cacggtgttg cgcgccgggg tggagcgcgc catcacgacg
     1441 cccgggatgc cgcaggaggc cctggccgac ctggaggaga cactccagga gatcaaccgc
     1501 atgaccgagc tcgtggacac tctgctgacg ctggcccggg ccgacgaggg gacggccccc
     1561 ctgcatctgg agccggtgga cctgcgggcc ctgttgctgg acaccgaaga gacggcggag
     1621 ctgctggccg agcccgcggg tgttacggtg agcgtcgagg cggtggaagg cccggtggtc
     1681 gtgcaggcgg acccgagccg cttccgccag ctcgtgatga acctgctcag caacgcggtg
     1741 aaatacacgc cccagggcgg gcgggtgcgg ctgtggctgg agcgcgacga cagccgtgtc
     1801 cgcttcggcg tgtccgacac cgggatcggg atcgcacccg gcgacctgcc gcacatcttc
     1861 caccggttct atcgggccga taccgcccgc actcgcaccg tcgatcgccc cggtgtgggc
     1921 ctcggcctcg ccatctgcaa atggattgcc gaagcgcacg gcggcacgat cgacgtgcag
     1981 agccggccag gacggggcac gacattcacg gtgacgctcc ctgcggcccc gaagccggca
     2041 tccgacgcga gcccggccaa gtcatgaggt gtccggcgct cgtcattctc tgctgcattc
     2101 tcccgctacg actgctcgcc caacacgatg cgggagatgc gctggtcctg ctcgccatcg
     2161 tgcgctacca ccagggcgac ggcgcggccg gcgtggccgc cttccgcgcg gcctgctcgc
     2221 cacgctgcga aggactcgac gttgcgccca tcgagatgcg ccccaaaacg cactgatcat
     2281 cccccagtca tcgaatcgtc atgcgaccag cacgtccccg acacgggcgt tgcccatcgt
     2341 agtggggaac tcctgatgcg aggtcgtcat gttcgacacg ctgctcgaga gtcgtcacac
     2401 tccactggga ttgccgcgct ggggcgtggc cgtcgccgtg ggtctgcacg ccgctgtggt
     2461 cggcgcggcg ctccgtcgct cgccgccccg c
//
LOCUS       DBNGZW010000115         3200 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1851978, whole genome shotgun sequence.
ACCESSION   DBNGZW010000115 DBNGZW010000000
VERSION     DBNGZW010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3200)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3200)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3200
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1851978"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..423
                     /locus_tag="AC16JC_02790"
     CDS             <1..423
                     /locus_tag="AC16JC_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003873319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR5163653.1"
                     /translation="LQQQEAIRELSTPVLQVRERLLILPIIGVIDPHRARQLTEQLLR
                     AIRTNRAKVVVIDITGVAAMDANVANHLVQTVEAARLLGATVIVTGLSPEIAQTLVTI
                     GVDLSEMATVGDLQGGIEEAERLLGYKVVPLTELTTQA"
     gene            446..820
                     /locus_tag="AC16JC_02795"
     CDS             446..820
                     /locus_tag="AC16JC_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR5163654.1"
                     /translation="MEVPILRQGAYLIASIQSALTDADLSQLREALIAQVGRYRARGV
                     IVDVTALDVMDSFAVRTLRDVAHMARLRGSETVIVGIQPEVAFAMVQLGLTLKGVATA
                     LDLEEGLAFLTQRARERVDHVH"
     gene            810..1265
                     /locus_tag="AC16JC_02800"
     CDS             810..1265
                     /locus_tag="AC16JC_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012934908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HIR5163655.1"
                     /translation="MSTDTTMRLDILTDKDVVCARQMGRELAAQFGFSPGDQTVIAAA
                     ISEIARNILLYARRGAVSFQLVDDGLRSAIVVVAQDEGPGIPDLTRALSDGYSTSGGL
                     GLGLPGAKRLMDEFDIVTAPGRGTTVTMSKVKRPGSVSRESRNGQGDTR"
     gene            1345..1872
                     /locus_tag="AC16JC_02805"
     CDS             1345..1872
                     /locus_tag="AC16JC_02805"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HIR5163656.1"
                     /translation="MNGGALVAVVDGIGHGEEAAAAARVAVDTLDRHAGESLPALVRH
                     CHDDLRGTRGVVMSVAHFDATHDMMTWVGLGNVEGVLVAGDHVVGRPYTSLVTRGGFV
                     GSAAAGLPPVRPWVIPIQRSDILVLATDGLRSDFGDDVTPSHSPQHTATLLLERYGKK
                     TDDALVLVARYLGRR"
     gene            1879..>3200
                     /locus_tag="AC16JC_02810"
     CDS             1879..>3200
                     /locus_tag="AC16JC_02810"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR5163657.1"
                     /translation="MSSPMSNPEPESIESAVVAEIAPAATLRCLAVGGEAAPAGQLRR
                     WAGELGVALEWTPDVPHAVRRLAVERWDAVLAVFSERPDEELAWWVDTLRAANAAQHL
                     IAVADRPSMGLALRAERLGVTDLLTRPVRREDLHAAIRRITSAATEASSPLPLVQPHA
                     VGPFALVGESPAMLEIYKLLAMVSGTTATVLLQGESGTGKEVIARTIHLSGARAAKPF
                     VAVNCAAIPENLLESELFGHEKGAFTGAVARKIGRFQLATGGTIFLDEIADLSLTLQA
                     KILRAVQEREIERLGGGEPIPVDVRLIAATNQDLRKAMEGGRFREDLYYRLAVMVIQL
                     PRLADRGDDLLSLVSYFAKTIGERYGKKITSISHRVLGVLRNRPWPGNVRELRNVVER
                     MVVVARGDTLCAEHLPAELRADDRVPADRHRGLLTLAEAEARHITRVLS"
BASE COUNT          472 a         1045 c         1183 g          500 t
ORIGIN      
        1 ctgcagcagc aggaagcgat ccgggagttg tctaccccgg tgctccaggt gcgcgagcgg
       61 ctcctgatcc tgccgatcat cggcgtgatc gatccgcatc gcgcccggca gctcaccgag
      121 cagctgctgc gcgcgattcg caccaatcgc gcgaaggtgg tggtgatcga catcacgggc
      181 gtggccgcga tggacgccaa cgtcgccaat cacctcgtgc agacggtcga ggcggcccgg
      241 ctgctgggcg cgacggtcat cgtcaccggc ctgtcccccg aaatcgccca gacgctggtc
      301 acgatcgggg tcgacctgag cgagatggcg acggtcggcg acctgcaggg cggcatcgag
      361 gaggcggagc ggctgctcgg ctacaaggtg gtcccgttga cggagctcac gacgcaggcc
      421 tgatcccgta gcgccggagg gcgtggtgga agtcccgatc ctcaggcagg gcgcctatct
      481 gatcgcctcg attcaatccg cgttgacgga cgcggatcta tcgcagttgc gggaggccct
      541 gatcgcccaa gtcggtcgct accgcgcccg tggggtgatc gtcgacgtca ccgcgctcga
      601 cgtcatggac tcgttcgccg tgcgcacact gcgggacgtc gcccacatgg cgcggctgcg
      661 cggctccgaa acggtgatcg tggggattca gcccgaagtg gcgttcgcga tggtccagct
      721 cgggttgacc ctcaagggcg tcgcgacagc gctggatctg gaagaggggc tggcgtttct
      781 gacgcagcgc gcccgcgagc gagtggacca tgtccactga cacgacgatg cggctcgaca
      841 tcctcaccga caaggacgtc gtgtgcgcga ggcagatggg gcgggagctc gcggcccagt
      901 tcgggttctc tcccggtgat cagaccgtca tcgcggcggc catctcggag atcgcgagga
      961 acatcctgct ctacgcgcgg cggggggcgg tgtcgttcca gctcgtcgac gacggtctcc
     1021 ggtccgcgat cgtggtcgtc gcgcaggacg agggaccggg gattcccgac ctgacacgcg
     1081 cgctgtcgga cggctattcg acgtcgggag ggctggggct cggcctgccg ggggcgaagc
     1141 ggctgatgga cgagttcgac atcgtcactg cgccagggcg tggcacgacg gtgacgatga
     1201 gcaaggtgaa gcggccgggc tccgtgagcc gggaatcccg caatggtcag ggcgacactc
     1261 gctagcgagt gcctcgacgt cggggtcgct tcctcggtac tccccggtca gcgcgagtcc
     1321 ggggacctcc agttggtgcg cctcgtgaat gggggcgcgc tcgtcgccgt cgtggacggc
     1381 attggacacg gggaggaagc cgccgccgcg gcgcgggtcg cggtggatac gctcgaccgc
     1441 cacgccggcg aatcgcttcc ggcgctggtg cgccactgcc acgacgacct gcgcggcacc
     1501 cggggggtgg tcatgagcgt ggcgcacttc gacgcgacgc acgacatgat gacgtgggtg
     1561 gggctcggca acgtcgaagg cgtcctcgta gccggagacc acgtcgtggg tcgcccctac
     1621 acgagcctgg tgactcgcgg cggcttcgtg ggcagcgccg cggcgggcct gccgccggtc
     1681 cggccctggg tgattccgat tcagcggagc gacatcctgg tgctcgccac cgatggactc
     1741 cgttccgact tcggcgacga cgtcacgccg agtcattcgc cgcagcacac tgccacccta
     1801 ctgctcgagc ggtatgggaa aaagacggac gacgccctgg tgctggtcgc caggtacttg
     1861 ggaaggcggt gaggcccgat gtccagcccg atgtccaacc cggagcccga gagcatcgag
     1921 agcgcagtgg tggccgagat cgcgccggcg gctaccctgc gctgcctggc cgtgggcggt
     1981 gaggccgccc ctgcgggcca gctgcggcgc tgggccggtg agctgggcgt ggcgctcgag
     2041 tggacgccgg acgtgcccca tgccgtgcgc cggctcgccg tggagcggtg ggacgcggtg
     2101 ctcgccgtgt tcagcgagcg cccggacgag gagctggcgt ggtgggtgga cacgctgcgt
     2161 gccgcgaacg ccgcgcagca cctgatcgcc gtcgcggacc ggccgtcgat ggggctggcg
     2221 ctccgggcgg agcggctcgg ggtgacggac ttgctgaccc gtccggtacg gcgggaggac
     2281 ctccacgccg ccattcggcg catcacttcg gccgccacgg aggcgtcgtc cccgctgccc
     2341 ctggtgcagc cacacgccgt cgggccgttc gcgctggtcg gcgagagccc ggcgatgctc
     2401 gagatctaca agctcctggc catggtctcc gggaccacgg cgacggtgct gctccagggt
     2461 gagtccggca cggggaagga agtgatcgcc cggacgattc atctgagcgg tgcccgtgcc
     2521 gccaagccgt tcgtcgccgt gaattgcgcc gccatccccg agaatttgct ggagagcgag
     2581 ctcttcgggc atgagaaggg cgctttcacg ggggccgtcg cgcgcaagat cggacgcttc
     2641 cagctggcca cgggggggac gatcttcctc gatgagatcg cggatctgag cctcacgctc
     2701 caggccaaga tcttgcgcgc cgtccaggag cgggaaatcg agcgcctggg tggaggcgag
     2761 ccgattcccg tcgacgtacg gctcatcgcg gccacgaacc aggatttgcg caaggccatg
     2821 gaaggcggcc ggttccgcga ggacctctat taccggctcg ccgtgatggt cattcagctg
     2881 ccgcgcctgg cggaccgggg cgacgacctg ctgtcgctgg tgtcctactt cgcgaagacc
     2941 atcggcgagc ggtacggcaa gaagatcacg tccatctctc accgggtgct cggcgtgctg
     3001 cggaatcggc cctggccggg aaacgtgcgc gagctccgca acgtcgtcga gcgcatggtc
     3061 gtggtggccc ggggagacac gttgtgcgcg gagcatctcc ccgccgagtt gcgcgcggat
     3121 gaccgggtcc ccgcggaccg gcaccggggg ctgctcacgc tcgccgaagc ggaggcgcga
     3181 cacatcaccc gcgtgctgtc
//
LOCUS       DBNGZW010000116        10117 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1442273, whole genome shotgun sequence.
ACCESSION   DBNGZW010000116 DBNGZW010000000
VERSION     DBNGZW010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 10117)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10117)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10117
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1442273"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="AC16JC_02815"
     CDS             complement(<1..1176)
                     /locus_tag="AC16JC_02815"
                     /inference="COORDINATES: protein motif:HMM:NF015724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HIR5163658.1"
                     /translation="MKRCLLFVLAALLGAGTLVAQAPAKRRVAVLDFDYATVHQYVYD
                     LFGSDQDIGKGIADMLVTNLVRNGAYSVIERKQLDRVLQEQNFQQSGRADPSSAVQIA
                     KILGVDAIIIGSITQFGRDDKKLGVGGIGARVGGIGLGGLGKKSAKAVVQIDARIVST
                     TTAEILGVATGHGESKRSSVSMVGGLAIGGTGVGGGLDMGSTNFANTIIGEATRAAVD
                     SLTGQLVATATTIPENVVQIQALVADVSGGEVTINVGTGAGIRVGGEYNVVRPGREIR
                     DPATGRVLRRVTTAVGKLKITQADEGSATGTLSGGPARVGDCVGTCPANTGGGGAAPP
                     PAVEPLNQGNAPAPPSGGGSLPALYSRPVTGPFTWGMYGFKGTEHFRYNVQQRDGSEN
                     "
     gene            1266..1910
                     /gene="upp"
                     /locus_tag="AC16JC_02820"
     CDS             1266..1910
                     /gene="upp"
                     /locus_tag="AC16JC_02820"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013007822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HIR5163659.1"
                     /translation="MSATRFPNLTVLDHPLIRHKLGLLRDRRTTTKDFKQLVSEIAML
                     MAYEVTKDLPLEPVDIDTPLERMLGERVAGKKLVLVPILRAGLGMVEGIAQLIPSARV
                     GHIGIYREHDTLKPVEYYFKIPRDGTARDFFILDPMLATGGSAVDAVTTLKRAGAQRV
                     RFLCLVACPEGVQRMHAAHPDVPIYTAALDRQLNDKGYILPGLGDAGDRLFGTR"
     gene            2000..2527
                     /locus_tag="AC16JC_02825"
     CDS             2000..2527
                     /locus_tag="AC16JC_02825"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5163660.1"
                     /translation="MISRVRAGEPAAERALYDAHVDRVYRLAFRLAGDDELARDFTQE
                     TFIRAFDRIGTFRGESRLSTWLHAIATTVSLNGLRKVKRFRTREMALEAADGVAGGVR
                     SAEPDLKTRLREAIDALPDKYRTVVVMHDMEGYTHEEIAAALDMEVGTSKAQLSRARA
                     KLRESLADFAGEWVS"
     gene            2524..3162
                     /locus_tag="AC16JC_02830"
     CDS             2524..3162
                     /locus_tag="AC16JC_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163661.1"
                     /translation="MNDDRFEQQINEAVRDYHRPPETPHEEMWAAIEAERRRRRTAPR
                     VVVLRPFWRWGIGMAAVLVLGIAIGRRLQSGTGGTPASPAAASSRPGDGLAYQLAAAQ
                     VLTRAEIVLTSFRADARTGGRPDSQFLDQARDLLGSTRLLLDSPAGQDPRLKPLLEDL
                     EVILAQIAVLSASRDTADVEIINQGIDQRSVLTRLQTANPAGLAPGHAQGAI"
     gene            3165..4769
                     /locus_tag="AC16JC_02835"
     CDS             3165..4769
                     /locus_tag="AC16JC_02835"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR5163662.1"
                     /translation="MYALSYLLAVTISTQDPATAAALDRAQAALPKLEAQLQATNASL
                     SATHLEGLARLDALGAQLGDLSALTQLSVWGDLSPIAGLSVLADLSHVPLGVWDDQDP
                     ADSLYRAGQRLLNRGRYAEAADSFAQLIRRHPRSQYTADAYYWEAFALYKTGDAGNYR
                     AARRVLQQQKARYPRAATAGDADALYARVQGELARQGDPDAAAWLRTHAAGAVPPTPP
                     TGPTPPTLPTPPAPPNGGRQTPAGCPSDDDDDRAAALNALLQMDDESAVPILKQVLAR
                     RDPCSVVLRRKAVFILSQKRTPETEDILLGVVRGDPDPEVREGGVFWLSQVGSERAVS
                     ALDSILRSSPDPELQKKAVFALSQIHSPRAGQILRDYAQRSDAPGEVREQAIFWLGQQ
                     HGEENAAFLRDLYARLTDEDLKDKVIFSISQMHSDENTRWLLDLALNEREPVEMRKKA
                     LFWAGQTGADMAGLVALYGRTTNEEMKDQLIFVYSQRHEPEAVTKLIDIALHESNQEL
                     RKKAVFWLGQSRDPRARQALLDIINQ"
     gene            4766..5572
                     /locus_tag="AC16JC_02840"
     CDS             4766..5572
                     /locus_tag="AC16JC_02840"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR5163663.1"
                     /translation="MSPRTFLAAAALSIGAAATAPAQALAARIAAARDGDVRFMYSSR
                     PDVWGNGRNAIQWNCRGGNCRQQVDGNRSDVNDDDWRSTCDTGPVRLTLRVRGGRVTA
                     LHAVVGGAWRPRSDATDLGRVAAAEAARYLIALARQAGGDVGNQAVFAATLADSVTTW
                     PDLLRLARDPEVPAGTRRSAVFWGSQAAEEAATRGLDSLAGDGGVDREVREQAVFALS
                     QRPKDEGVPALIRIATTHTDPEIRRRAIFWLGQSNDPRALDLFETLLTIP"
     gene            5670..6209
                     /locus_tag="AC16JC_02845"
     CDS             5670..6209
                     /locus_tag="AC16JC_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR5163664.1"
                     /translation="MQVSTRRVYTGRILNLDVDTVRFPDGSEGELEMIRHPGAAAVVP
                     LASDPTGPDPVILMIRQYRYAAGGPLWEIPAGRLDPGEAPEACAHRELREEAGVTAGR
                     LDRLTTIWTTPGFTDEAIHLFLAQDLEAATAAREPDEFIEVVPRSLSEVLAGIRDGEI
                     CDGKTIAGILYVAGFRLGM"
     gene            6355..6984
                     /locus_tag="AC16JC_02850"
     CDS             6355..6984
                     /locus_tag="AC16JC_02850"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5163665.1"
                     /translation="MTTTRLDPSPRAVERQTLHELDDAQVVQRHLNGDPQAFGMLVDR
                     YQGRLLSFVNRTIGDRERAEDLVQEVFIRVFRHLHRFDQTKKFSTWIYTIASNLAKNE
                     LRNRSRNPLVFFQTIKRNWEADHRPLQFEDATARPDDLYRKRFLKDAVEQCVSQLPEH
                     HRVVFVLRELEGKSYEEIAEITGCNLGTVKSRLNRARNSFAQLIGPLVS"
     gene            7027..7524
                     /locus_tag="AC16JC_02855"
     CDS             7027..7524
                     /locus_tag="AC16JC_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR5163666.1"
                     /translation="MNCREFLARYTDFRDGLISAPRESRQFTRHLASCASCRRYDAAL
                     RHGVGALQDAPNHSPDEHFRHRLERRIAAERVRAGRPHIPTRPALAGLLLVAAAVTLV
                     VWEGARRHPVEPLAQSLPPVPFPKPVAQAGVPFVSFQDPRTGIATGNPYPYGTAFVEP
                     ASASR"
     gene            7571..8623
                     /gene="holA"
                     /locus_tag="AC16JC_02860"
     CDS             7571..8623
                     /gene="holA"
                     /locus_tag="AC16JC_02860"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HIR5163667.1"
                     /translation="MAPLSFDALSRALKRGEPEAVYYLHGDEAVLKDEAINALIERAV
                     PPESRDFNLDVRTAAELDVEALHLLLNTPPMLAPRRAVVLRGVEQLRKKSTARDALLA
                     YLEHPSPETLLVLVQTDTEPPEADLAARSTTVTVERLPPERAARWVVHAAGRVGLAVE
                     PAAADLLVESLGADLSALRQELDKLAVLVIGRPVTPTDVASLVGIRSGETLHDLIAAT
                     LSREPGRAARMVDGVLAQSGMSGVRIVTAVGSALVGTALARAELDRGTPQGRLADTVF
                     RHLLAARPFGLRSYKVEAAQWTAWAPLWDAADLRHALRLALVADQALKSSGISDDAGV
                     VRQLVLSLCVPALEAA"
     gene            8623..9456
                     /locus_tag="AC16JC_02865"
     CDS             8623..9456
                     /locus_tag="AC16JC_02865"
                     /inference="COORDINATES: protein motif:HMM:NF016895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HIR5163668.1"
                     /translation="MRARLTVPVVAALLGLASELPGQAPAQPQFEPRLQAAITLAQSG
                     RADSARALVRTLIASLTPQDPLLPQALYVQGGMLAPDANVAATSLQRVVVEYGTSAWA
                     DDALLRLAQLYEAQNDPASAVQSVERLRRDYPGSPLLARADFVGARAAFDLRDEARGC
                     DLLHDAQRGTGDDVEFRNQVAFYSARCAGTTAVAAPVATTTRVGRFAVQVLAGKSAPQ
                     VDEMLTRLKGLGYAAHVVRDSSGYLKVRVGPYVSRDAAQRAQTQLKTRLGGQPFVVEE
                     P"
     gene            9453..>10117
                     /locus_tag="AC16JC_02870"
     CDS             9453..>10117
                     /locus_tag="AC16JC_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013112299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MutS N-terminal domain-containing protein"
                     /protein_id="HIR5163669.1"
                     /translation="MSSSVTDTPLMQQYREIKARHPQTILFFRMGDFYEMFEDDAKLA
                     ARELGLTLTSRNNGGAAEVPLAGVPVKAATEYLRRLIAKGHRVAICEQVEDPRLAKGL
                     VRREVIETLSPGAVLADDWLERNRNNFLVALAERGDVVGLAALDLTTGEFALEVVPQE
                     ELGAALARYEAVELVVAAGGPVPRSAPESRMVTEREAWEFDSELAREDLVRTFGLVSL
                     DGLG"
BASE COUNT         1630 a         3509 c         3374 g         1604 t
ORIGIN      
        1 gttctcgctg ccgtcgcgct gctgcacgtt gtagcggaag tgctcggtcc ccttgaagcc
       61 gtacatcccc caagtgaacg ggcccgtcac cggcctgctg tagagcgccg gcaggctgcc
      121 gccccccgat ggtggggccg gtgcgttccc ctgattcaac ggctcgaccg cgggtggcgg
      181 cgcagcgcca ccacccccgg tattggccgg gcaggtcccc acgcagtcac cgacacgggc
      241 ggggccgccg ctgagcgtgc ccgtcgcgga tccctcgtcc gcctgggtga tcttgagctt
      301 cccgaccgcc gtggtaacgc gccgcaacac gcgtcccgtg gccggatcgc ggatctcacg
      361 gccggggcgg acgacgttgt actccccgcc cacccggatg cctgcgccgg tgccgacgtt
      421 gatcgtcacc tcgccgccgg acacgtccgc caccagcgcc tggatctgga cgacgttttc
      481 ggggatggtg gtagcggtgg ccacgagctg gcccgtcaga ctgtccaccg ccgcacgggt
      541 cgcttcaccg atgatggtat tggcgaagtt ggtgctgccc atgtcgagcc cgccgccgac
      601 ccccgtcccg ccgatcgcca atccgccgac catcgacacg ctggagcgct tggactcgcc
      661 gtgacccgtc gccacaccca ggatctcggc cgtcgtcgtg ctcacgattc gggcgtcgat
      721 ctgcacaacg gccttggcgg acttcttgcc gagcccgcca agcccgatgc cgccgacccg
      781 cgccccgata ccacccacgc cgagcttctt gtcgtcacgg ccgaactgcg tgatgctgcc
      841 gatgatgatg gcatcgacgc cgagaatctt cgcaatctgc acggccgagg atggatcggc
      901 gcgcccggac tgctggaagt tctgctcctg cagcacccgg tcgagctgct tgcgctcgat
      961 caccgagtag gcgccgttgc gcacgagatt cgtgaccagc atgtcggcga tccccttgcc
     1021 gatgtcctgg tcgctgccga acaggtcgta gacgtactgg tggacggtgg cgtaatcgaa
     1081 atccaacaca gccacccgcc gcttggcagg agcctgcgcg accagtgtcc cggcacccag
     1141 caacgcggcg aggacgaaca gcagacagcg cttcatgacg acaccccttc gaggatgaga
     1201 gcgcgaagcg gacccggtga ttctagcgcg caagacacaa gtttcaacta gttttcgtct
     1261 tcacaatgtc cgccacccgg ttccccaatc tgaccgtgct cgatcacccg ctgatccggc
     1321 acaagctcgg cctgttgcgc gaccgccgca cgacgaccaa ggacttcaag caactcgtca
     1381 gcgagatcgc gatgctgatg gcgtacgagg tcaccaaaga cctgccgctc gaacccgtgg
     1441 acatcgatac gccgctcgag cgtatgctgg gagagcgcgt cgccggcaag aagctggtgc
     1501 tcgtgcccat cctgcgcgcg ggcctcggca tggtcgaagg catcgcccaa ctcatcccgt
     1561 cggcccgcgt cggacacatc ggtatctacc gcgagcacga caccctgaag ccggtcgagt
     1621 actacttcaa gattccccgc gacggcaccg cccgcgattt cttcattctc gaccccatgc
     1681 tcgcgaccgg cggctccgcc gtcgacgccg tcaccaccct gaagcgggcc ggtgcgcagc
     1741 gcgtccgctt cctctgtctg gtcgcctgcc ccgaaggcgt ccagcgcatg cacgccgccc
     1801 accccgatgt gcccatctat acggccgcac tcgaccgcca gctcaacgac aagggctata
     1861 tcctccccgg ccttggagat gcaggcgaca ggctgttcgg gacgaggtga cgctccgctt
     1921 ccccgtcgca actgtttttc cccatcccgt gtcacaggat tgaacgactc ccgtacggag
     1981 cagcgtggac gaatctgaat tgatctcccg tgtccgcgcg ggagagccgg cggcggaacg
     2041 tgcgttgtac gacgcacatg tcgaccgcgt ctatcgcctc gcgttccgcc tcgccgggga
     2101 cgacgaactg gcccgggatt tcacgcagga aacgttcatc cgggcgttcg atcgcatcgg
     2161 cacgtttcgc ggcgagtccc ggctctcgac gtggctgcac gccatcgcga ccaccgtgtc
     2221 gttgaacggg cttcggaagg tgaagcggtt ccggacgcgg gagatggcgc ttgaggcggc
     2281 cgacggtgtg gccggcggcg tgcggtcggc cgagcccgat ctgaagacca ggctgcgcga
     2341 ggccatcgac gcgctgccgg acaagtacag gacggtggtt gtgatgcacg acatggaagg
     2401 ctacacgcac gaggagatcg cggccgcgct ggatatggaa gtcggcactt cgaaggccca
     2461 gttgtcacgg gcacgagcca aattgcgcga gtcgctggcg gattttgcgg gagaatgggt
     2521 gtcatgaacg acgatcggtt cgaacaacag atcaacgagg ccgtgcggga ctaccatcgg
     2581 ccgcccgaga cgccgcacga ggagatgtgg gccgcgatcg aggctgagcg ccggcggcgc
     2641 cgcaccgcgc cacgcgtcgt cgtgttgaga cccttctggc gttggggaat cggcatggct
     2701 gcggttctgg tgctcgggat cgcaatcggg cggcgcctcc agtccgggac gggcggaaca
     2761 ccggcatctc ccgcagccgc ttcgagccgg cccggcgacg gcctcgccta tcagctggcc
     2821 gccgcgcagg tcctcacccg agccgagatc gtgctcacga gcttccgcgc cgacgcacga
     2881 accggggggc gccccgactc gcagtttctc gaccaggccc gcgacctgct cgggtcgacc
     2941 cggctgctgc tcgactcgcc agcggggcag gatccgcgtc tcaaaccact gctcgaggac
     3001 ctcgaagtga tcctcgcgca gatcgcggtg ttgtccgcgt cgcgggatac cgccgacgtg
     3061 gagatcatca accagggaat cgaccaacgg agcgtgctca cccggctgca aaccgcgaac
     3121 cccgcgggcc tcgcaccggg gcatgcacaa ggagcgattt gacgatgtac gcgctgtcct
     3181 acctactggc cgtcacgatt tcgacccagg atccggcgac cgcggccgcg ttggaccgcg
     3241 cccaggcggc gctgcccaag ctggaggctc agctgcaagc gacgaacgcg tcgctgagcg
     3301 cgacccacct cgaaggcctc gcgagactcg atgcgctggg ggcccagctc ggcgaccttt
     3361 ccgccctcac gcagctgtcg gtttggggtg atctcagccc aatcgccggg ttgtccgtgc
     3421 tcgcggacct gtcgcacgtg ccgcttgggg tgtgggatga tcaggatccc gccgactcgc
     3481 tgtatcgcgc cgggcagcgg ctcctcaacc gtggtcgcta cgccgaggcc gcagactcct
     3541 ttgcgcagct gatcaggcgc cacccgcggt cccaatacac ggcggacgcc tactattggg
     3601 aggcatttgc gctgtacaag accggcgacg cgggcaacta ccgcgcggcg cgccgtgtgc
     3661 tgcaacaaca gaaggcccgc tatccgcgag ccgcgacggc cggcgacgcg gatgcgctct
     3721 acgcgcgggt gcagggcgaa ctcgcccgcc agggagaccc ggacgccgcg gcgtggctgc
     3781 ggacgcatgc ggccggcgcg gtaccgccca ccccgcccac cggcccgacg ccgcccacac
     3841 tcccgactcc acccgcgcca ccgaatggcg gcaggcagac accggcgggc tgtccgtccg
     3901 acgacgatga cgaccgggcg gctgcgctca acgccttgtt gcagatggac gacgagtccg
     3961 ccgtaccgat cctgaaacag gtcctggcac gccgcgatcc gtgcagcgtg gtgttgcggc
     4021 gcaaggcggt gttcatcctg tcccagaaac ggacgcccga gaccgaggac atcctcctcg
     4081 gcgtcgtccg cggcgacccg gatccggaag tccgtgaagg gggggtgttc tggctgtcgc
     4141 aggtggggag cgagcgggcc gtgagtgcgc tcgattcgat cctgcgctcc tctccggacc
     4201 cggagcttca gaagaaagcg gtgttcgccc tctcgcagat ccatagtccg cgagcgggtc
     4261 agattctgcg cgactatgct cagcgcagcg acgcccccgg agaagtccgc gagcaggcga
     4321 tcttctggct gggccagcag cacggcgagg agaacgccgc attcctgcgg gatctgtacg
     4381 cccgcctgac ggacgaggat ctgaaggaca aggtcatctt ttcgatcagc cagatgcact
     4441 ctgacgagaa cacccgctgg ctgctcgacc tggcgttgaa cgagcgtgag cccgtcgaaa
     4501 tgcgcaagaa ggcgctgttc tgggccgggc aaaccggtgc cgacatggcc ggcctcgtgg
     4561 ccctctatgg ccgcacgacg aatgaggaga tgaaggacca gctgatcttc gtctactcgc
     4621 agcgacacga gcccgaggcg gtgaccaaac tgatcgacat cgccctgcac gagagcaacc
     4681 aggagctcag gaagaaggcc gtgttctggc tgggccaatc tcgcgaccca cgcgcccgtc
     4741 aggcgctgct cgacatcatc aaccaatgag cccgcgcaca ttcctggcag cggccgccct
     4801 gtcgatcggg gcggccgcga cggccccggc gcaagccctc gccgcacgga tcgctgcagc
     4861 gcgcgacggc gacgtccggt tcatgtattc ctcccggccc gacgtctggg gcaatggccg
     4921 caacgccatc caatggaact gccggggtgg gaactgccga cagcaggtag acggcaaccg
     4981 ctccgacgtc aacgatgacg actggcgttc cacatgcgac acgggtcccg tgcggctgac
     5041 gctgcgcgtg cgcggcggac gtgtcaccgc cctccacgcg gtggtcggcg gtgcctggcg
     5101 cccccgttcc gatgccaccg acctgggccg cgtcgcggcg gctgaagcgg cacgctacct
     5161 catcgcgctg gcccgccagg ccgggggcga cgtcggcaac caggccgtct tcgccgccac
     5221 tctggccgac agcgtcacga cctggcccga cctgctgcga ctcgcccgcg accccgaggt
     5281 cccggctggg acgcgccgtt cggccgtctt ctggggatcg caggccgccg aggaagccgc
     5341 gacgcggggg ctcgactcgc tcgcgggcga cggcggcgtc gatcgcgaag tgcgcgaaca
     5401 ggcggtcttc gccctgtcgc agcgccccaa ggacgagggt gttcctgccc tgatccgcat
     5461 cgccacgacc cataccgatc ccgagatccg gcgtcgggcg atcttctggc tgggccagtc
     5521 gaacgaccct cgcgccctcg acctgttcga gaccctgctg accataccct gaccccgggc
     5581 gccgtaccga tacggtccga atgtgttccc ttgaaggcgg aggggacatt cggaccggta
     5641 tattgcgcgg tctcccatcc ggacgaccga tgcaagtgag cacgcggcgt gtgtacaccg
     5701 ggcggatcct gaacctggac gtcgatacgg ttcgatttcc ggacggctca gagggcgagc
     5761 tcgaaatgat ccgccatccg ggggcagccg cggtcgtacc gctggcgtcg gaccccacgg
     5821 ggcctgaccc ggtgatcctg atgatccgcc agtaccgtta tgccgcgggt ggccccttgt
     5881 gggagatccc cgccggacgg ctggacccgg gcgaagcgcc ggaagcatgc gcgcacaggg
     5941 aattgcgcga ggaggcggga gtcaccgccg gccggctcga ccggttgacg acgatctgga
     6001 ccacaccggg attcaccgac gaggctattc atctgtttct cgcacaggac ctcgaagccg
     6061 cgacagccgc ccgcgagccg gatgagttca tcgaggtggt cccgcgctcg ctgtccgagg
     6121 tgcttgcggg gatacgggac ggggagatct gcgacggcaa gacgatcgcg ggcatcctgt
     6181 acgtggcggg atttcgcctt gggatgtaat gacgcactgt ccccgggaga ggacgggaat
     6241 gagacctata gtcttgccta atgccttcct taatcggatc ttagggttgg gttggtacgc
     6301 ggtacggttc ctgcccggaa cgtcggggcg tacttggagg ggggggatat gccggtgact
     6361 acgaccaggc tggatccgtc gccacgagcg gttgagcgcc agacgctcca cgagctggac
     6421 gacgcgcaag tcgtccagcg gcacctcaat ggcgacccgc aggccttcgg gatgctggta
     6481 gaccgctacc agggccgttt gctgagcttc gtcaaccgca cgatcgggga ccgggaacgg
     6541 gcagaggacc tcgtccaaga ggtcttcatc cgcgtgtttc gtcacctcca tcggttcgac
     6601 cagaccaaga agttctcgac ctggatctac acgatcgcat cgaaccttgc gaagaacgag
     6661 ctgcgcaacc gcagccgtaa tccgctcgtg ttcttccaga cgatcaaacg gaattgggaa
     6721 gccgaccacc ggccgctgca gttcgaggat gcgacggccc gccccgacga cctgtaccgc
     6781 aagcggttcc tcaaggacgc cgtggaacag tgcgtgagtc agctgccgga gcatcaccgg
     6841 gtagtgttcg tgctgcgaga gctcgagggc aaaagctacg aggaaatcgc cgagatcacc
     6901 ggctgcaacc tcggcactgt gaagagccga cttaaccgcg cgcgtaacag ttttgcacag
     6961 ctgatcgggc cgctggtgtc ctgaggcggg acccccagcc gcgccgccgg gtacgattca
     7021 gccgtcatga actgccggga attcctcgcc cgctacacag acttccggga cgggctcatc
     7081 agcgcgcccc gggagtcgcg tcaattcacc cgtcacttgg cgagttgtgc gagttgccgg
     7141 cgctacgatg cggcactgcg tcatggtgtg ggtgcgctgc aggacgcccc gaatcactcg
     7201 cccgacgagc atttccggca ccgtctcgag cggcgcatcg ccgcggagcg ggtgcgggcc
     7261 gggcggccgc atatccccac ccgccccgcc ctagccggtt tgttgctcgt cgcggccgcc
     7321 gtaacgctcg tcgtctggga aggcgcgcgg cgacacccgg tggagccgct ggctcagtcg
     7381 ctccctccgg tgcccttccc gaaacccgtg gcgcaggcgg gcgtgccgtt cgtgtcgttt
     7441 caggatccgc ggaccggaat cgcgacgggc aacccctatc cgtacggcac ggcgttcgtc
     7501 gaaccggcga gcgcttcgcg ctgaccgcct cactttcttc ccctacgagg cggcggtacc
     7561 ttccgtcgct atggcgccgc tctccttcga cgcgctgtct cgcgcgctca agcgcggaga
     7621 gccggaggcc gtctactatc tccacggtga cgaggcggtc ctcaaggacg aggcgatcaa
     7681 cgccctgatc gaacgcgccg tcccacccga gtcgcgtgat ttcaatctgg acgtacggac
     7741 ggctgccgaa ctggacgtag aggcgctcca cctgctgctc aacacgcccc cgatgctcgc
     7801 gccacgccgg gccgtcgtcc tgcgtggggt cgagcagctc cgcaagaaat ccacggcgcg
     7861 cgacgcgctg ctcgcctacc tcgaacatcc gagccccgag acactcctcg tgctggtaca
     7921 gaccgacacc gagccccccg aggccgacct cgccgcgcgc tccaccacgg tgacggtgga
     7981 gcggcttcca cccgagcggg ccgcccgctg ggtggtccat gccgcggggc gggtgggcct
     8041 cgccgtcgag ccggccgccg ccgacctgct cgtggaatcg ctcggcgccg atctctcggc
     8101 actgcgccag gagctggaca agctcgcagt cctagtgatc ggccgcccgg tgaccccgac
     8161 ggacgtcgcc tcgctggtgg gcatccggag tggggagacg ctgcacgacc tcatcgccgc
     8221 gacgttgtcg cgggagccgg gccgtgcggc gcggatggtc gatggagtgc tggcgcagtc
     8281 tgggatgtcc ggcgtcagga tcgtcaccgc cgtgggctcg gccctggtgg gtacggcatt
     8341 ggcccgtgcc gagctcgatc gcggcacgcc ccaggggcgc ctggccgaca cggtgtttcg
     8401 tcacctgctc gccgcccgcc cgttcgggtt gcgcagctac aaggttgaag cggcccagtg
     8461 gactgcgtgg gcaccgttgt gggatgcggc cgatctgcgc cacgcgctgc gcctcgcgct
     8521 cgtcgccgat caggcgctga agagctccgg catctcggac gacgccggcg tcgtccgaca
     8581 actcgtgctc tcgctctgtg ttccagcgct ggaggccgct tgatgcgcgc gcgactgacc
     8641 gtccccgtgg tcgcggcgct cctgggcctc gcgtccgagc tgccgggaca agcgccggca
     8701 caacctcagt tcgagccgcg gctccaggcc gccatcaccc tggcgcagtc cggccgcgcc
     8761 gattcggccc gggcgctggt gcgcacgctg attgcttcgc tgaccccgca ggaccccctc
     8821 ctccctcagg ccctgtacgt gcagggcggg atgctcgccc ccgacgcgaa cgtggcggcg
     8881 acgagtttgc agcgcgtggt ggtggaatac ggcacctcgg cctgggcgga cgacgcgctc
     8941 ctgcgcctcg cacagctgta cgaggctcaa aacgatccgg cgtcggcagt ccagtcggtg
     9001 gaacgcttgc gccgcgacta tcccggctcg cccctgctcg cccgtgcgga tttcgtcggg
     9061 gcgcgtgcgg ccttcgacct ccgtgacgag gcacgcggct gcgacttgct gcacgatgcc
     9121 cagcgcggca ccggggacga cgtcgagttc cggaatcagg tcgcgttcta ctccgcacgg
     9181 tgcgcgggaa cgaccgccgt cgccgccccg gtggcgacta ctacccgcgt cggccgcttc
     9241 gccgtgcagg tcctcgccgg taagagcgcg cctcaagtgg acgagatgct cacacggctg
     9301 aagggcctgg gctatgcggc acacgtggta cgcgactcct cgggatacct gaaagtccgg
     9361 gtgggtccgt acgtctcgcg cgacgccgcg caacgcgccc agacgcagct caagacgcga
     9421 ctcggcggac agccgttcgt ggtggaggag ccgtgagctc gtcggtcacc gacaccccgc
     9481 tgatgcagca gtaccgggag atcaaagcac ggcaccccca gaccatcctg ttcttccgga
     9541 tgggcgactt ctacgaaatg ttcgaagacg acgcaaagct cgcggcccgg gagctggggc
     9601 tgacgctgac gtcgcgcaac aacggcggcg ccgcggaagt cccgctcgcg ggagtccccg
     9661 ttaaagccgc gaccgagtac ctgcgtcgcc tgatcgccaa aggccaccgc gtggccatct
     9721 gcgaacaagt cgaagatccg cgactcgcca agggactggt gcgccgcgaa gtgatcgaga
     9781 cgctgtcacc gggggcggtg ctcgccgacg actggctgga gcgcaaccgt aacaatttcc
     9841 tcgtggcgct cgccgagcgc ggcgacgtcg tcggcctcgc ggccctggac ctgacgacgg
     9901 gggagttcgc gctggaggtc gtgccacagg aggagctggg cgcggcgctg gcgcggtacg
     9961 aggcggtgga actggtggtg gccgccgggg gaccggtccc acgatccgcc ccggagtcaa
    10021 ggatggtgac cgagcgagag gcctgggaat tcgactccga gctcgcccgc gaggatctgg
    10081 tgcggacgtt cggactcgtc tccctcgacg gactggg
//
LOCUS       DBNGZW010000117         3123 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1510154, whole genome shotgun sequence.
ACCESSION   DBNGZW010000117 DBNGZW010000000
VERSION     DBNGZW010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3123)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3123)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3123
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1510154"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="AC16JC_02875"
     CDS             <1..254
                     /locus_tag="AC16JC_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163670.1"
                     /translation="RALIAEFAGPEHGVIDDETPLISSALVESIALLNVALWVEEQID
                     AAVEITAFDLASEWDTVARILDFVEKHRGHDRAGPRSSA"
     gene            251..1378
                     /locus_tag="AC16JC_02880"
     CDS             251..1378
                     /locus_tag="AC16JC_02880"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5163671.1"
                     /translation="MTYEIVRYEPAFRDQVAELQRHLWGDDASINAAYLDWKYHQNPY
                     LDEPLICLAVDAGRVVGMRGMFGALWEIGTPAEPVVVPCADDFVIAPEHRNRGLVAQI
                     MNATVDRVAAEGHRYAFSLSAGPVTMMASRATGWRGAGSMRAAQRKGVDGLLTRASAR
                     LRTLPLLWRYADPLARLQYRLQPPFARLDREARRAGGPLDAGVRVEPAPRVDAMADLV
                     RRLGHDGRLRHVRDERYLAWRYRNPLYEYRFLFRESGRLESYLVLRADRQDPARGVNI
                     VDWEAATPALREELLRAAVAWGSFPELTIWTATVPPDTLAGVSRAGFVPMGAGSRTRS
                     SHSILVCDARRPRPAPDMILGGRRLLDPNTWDMRMVYSMAG"
     gene            1800..2198
                     /locus_tag="AC16JC_02885"
     CDS             1800..2198
                     /locus_tag="AC16JC_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163672.1"
                     /translation="MNDEPESLGGHVARLALVVAALLVVVGAGWTWLTLSWAYSEGER
                     AGVLQKFSKKGWICKTYEGEVAQYVVGGLAPQIWYFSVRDAALAEQLFKAVGQNVQLH
                     YSEHRGIPTNCFGDTPYFAQSFAIIPTATR"
     gene            2336..2623
                     /locus_tag="AC16JC_02890"
     CDS             2336..2623
                     /locus_tag="AC16JC_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018076342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163673.1"
                     /translation="MRKGQRIAAAVAVGVLLWSGSALAQGKPTCTSQGKLQTAQKVVG
                     QVVKVDAGLDKLTVREDDGTVHEFQASKEALREFKAGDRIEAMLREAPKCP"
     gene            2729..>3123
                     /locus_tag="AC16JC_02895"
     CDS             2729..>3123
                     /locus_tag="AC16JC_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163674.1"
                     /translation="MPDRASRVGAAPILPDRSVAGDARPAWRLVRWRDAALACLAAVA
                     AALGGCATAAPAGPPPAPDYARPAAPGGPLAMLEATVRDASGPDASGFRLLERNEDGL
                     RWRLALVDSARSTLDLQYYVWAGDTSGLLL"
BASE COUNT          479 a         1035 c         1129 g          480 t
ORIGIN      
        1 tgagggcgct gatcgccgag ttcgccggcc cggagcacgg ggtgatcgac gacgagacgc
       61 ccctgatcag ctcggcgctg gtggaatcga tcgcgctgct caacgtggcc ctctgggtcg
      121 aagagcagat cgacgccgcg gtcgagatca ccgccttcga cctcgcctcg gagtgggata
      181 ccgtcgcccg gatcctggac ttcgtcgaga agcaccgcgg ccacgaccgc gcggggccac
      241 ggagctccgc gtgacgtacg agatcgtccg gtacgagccc gcgttccggg accaggtggc
      301 cgagctccag cgtcacctct ggggcgacga cgcctccatc aacgccgcgt acctcgactg
      361 gaagtatcac cagaacccgt acctcgacga gccgctcatc tgcctggccg tggacgccgg
      421 gcgggtggtc gggatgcgcg gcatgttcgg cgcgctctgg gagatcggca cgccggccga
      481 gcccgtcgtg gtgccctgcg cggacgactt cgtgatcgcg cccgagcatc gcaatcgcgg
      541 gctggtcgcc cagatcatga acgcgaccgt cgaccgtgtc gcggccgaag gtcaccgcta
      601 cgcgttcagc ctgagcgccg gcccggtcac catgatggcg tcgcgcgcca ccggctggcg
      661 gggcgccgga tcgatgcggg ccgcgcagcg gaaaggggtg gacggattgc tcacgcgggc
      721 ctccgcccga ctgcgcacgc tgccgctgct gtggcgctac gccgatccgc tcgcgcgcct
      781 gcagtatcgg ctgcagccgc cgttcgcccg tctggaccgc gaggcccggc gcgccggcgg
      841 cccgctcgat gccggcgtac gggtcgagcc ggcgccccgg gtcgacgcga tggcggacct
      901 cgtgcgccga ctcgggcacg atgggcggct ccgccacgtc cgggacgagc gctacctggc
      961 ctggcggtac cgaaatcccc tctacgagta tcggttcctg ttccgggagt ccggtcggct
     1021 ggagagctat ctggtcctgc gggcggaccg acaggatccg gcccgcggcg tcaacatcgt
     1081 ggactgggag gcggccacgc ccgcgcttcg cgaggagctg ctgcgcgcgg cggtggcctg
     1141 ggggagcttc cccgagctga ccatctggac cgcgacggtc ccgcccgaca cgctcgccgg
     1201 ggtctcccgc gccggattcg tgccgatggg cgccggctcg cggacgagat cttcgcacag
     1261 catcctggtc tgtgatgctc ggcgaccgcg gccggcgccg gacatgatcc tgggagggcg
     1321 gcgcctgctc gacccgaaca catgggatat gcggatggtc tactccatgg ccggatgagc
     1381 ggatgagcct ccgggtcaat cggaggggcc attggaccta ggccccatag tcggccctgt
     1441 gaaccggcta ccgtgtggcg cgggtcacac gcgcgacgag cagaggactc cgaagacgca
     1501 tcttgactgc ccccggttgt cgacgcaaga tcgcagtcga tgaatggcct ggctggcggg
     1561 tcgacgatgc ggtccggcca cggattgatg cgctcccgtt gtgcgctctc ggcgtatcca
     1621 ggcacgatcg cggcccggca ggctcggcct cgccgaggac ccgcaaacac tgcgatcctc
     1681 acgcccccca ggctctcgca tacgggacat acgatgggac atacgacggc gtggcgtcgg
     1741 catcggttct gagcccgtcg gcggtcgacc acacatggtg gggaacgagg gagcggagcg
     1801 tgaatgacga gccggaaagc ctaggcggac acgtcgcgcg acttgcactg gtagtcgcgg
     1861 cgttgctcgt cgtagtcggg gccgggtgga catggctcac cttgtcgtgg gcgtattccg
     1921 aaggtgagcg cgccggcgtg ttgcagaagt tctccaagaa gggctggatc tgcaagacct
     1981 acgagggtga agtcgcacaa tatgtcgtcg gaggcctcgc gccgcagata tggtatttca
     2041 gcgtgcgcga tgcggcgctg gccgagcagc tcttcaaggc cgtggggcag aatgtgcagc
     2101 tgcactacag tgaacatcgc ggcatcccca ccaattgctt cggcgatacg ccctacttcg
     2161 cccagagctt cgccatcatt ccgaccgcga cacgatagcc ttgggcaagg cggcaactgg
     2221 tgtggcggga gggccggcac cggatacggc gccggtaagg gcgtcctcat cggcaccggc
     2281 gtgggggccg cggcgggtgc catctacgac ttcacaaaga aatagggagg ggacgatgcg
     2341 caagggccag cggatcgcag cggccgtggc ggtgggagtg ctgctctgga gtggaagcgc
     2401 actggcgcag ggcaagccga cctgtacgtc ccagggaaag ctccagacgg cgcagaaggt
     2461 cgtggggcag gtcgtcaagg tggatgcggg cctcgataag ctgacggtac gagaggacga
     2521 cgggacggtg cacgagttcc aagcctcgaa ggaagcgctt cgggagttca aggcagggga
     2581 tcgcatcgag gcgatgctcc gcgaggcccc caagtgcccg taaggtaccg ggacgccggc
     2641 cctctgaccg attgacgaga ggagttgctc gagcgctgag gccctctgga tcgcgcgcgg
     2701 cccagagtct gctagactcc cgcgcgttat gccggatcgt gcttcccggg taggtgccgc
     2761 gccgattctg cctgaccgct cagttgctgg cgacgcgaga ccggcgtggc ggctcgtccg
     2821 ctggcgggat gccgcgctcg cctgtctcgc ggcggtcgcg gctgcgttgg gcggctgcgc
     2881 taccgcggcc cccgccggac caccgccggc ccccgactac gccaggccgg cggcaccggg
     2941 cgggccgctc gcgatgctcg aggccacggt gcgtgacgcg agcggaccgg acgcttccgg
     3001 gttccgactc ctcgagcgca acgaggacgg tctccgatgg cgcctcgccc tcgtggactc
     3061 cgcccgctcc acgctcgatc tgcagtatta cgtctgggcc ggcgacacga gcggactcct
     3121 gct
//
LOCUS       DBNGZW010000118         3422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1029368, whole genome shotgun sequence.
ACCESSION   DBNGZW010000118 DBNGZW010000000
VERSION     DBNGZW010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1029368"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1170
                     /locus_tag="AC16JC_02900"
     CDS             <1..1170
                     /locus_tag="AC16JC_02900"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR5163675.1"
                     /translation="TRDSVIGRIQSQYVLAIVADTSPNMWISTLKGIGHSPDAGRTWH
                     EHFPPAPCTGVGCVNRARALSLDSGGRFWIGTEQGLFRFGPEFGGWATPDGQPCGKRA
                     SCPGGPLPVRAFTSRTLRPPGEAPRSSPPEKPRHTWFRRPIALADQPYIDQTYRFGST
                     MGGNFQPHQGVEFNNPDGTPVHAIGDGVVVYSGPAEAGANTVAIRHDRPLKTDDGRRQ
                     YVFSVYYHNTKLVATVGQRVKAGDVISLVGNTGRATNDHLHLEVHVAPTTDSSKIVDA
                     AVRYPPFNTNPELWIDPLPGTGLVAGQVWDSHGEPVQQARIYGLVKPEPQETPFSFIE
                     TYGARNHSDPVYHEHFAIGDVPPGEYDLGVEIEGRKVYRRVRVTAGKLTWVEFHP"
     gene            1211..1834
                     /locus_tag="AC16JC_02905"
     CDS             1211..1834
                     /locus_tag="AC16JC_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163676.1"
                     /translation="MHIELTEMLRCPEDHREEFLVLSTSEMNGRMVMRGVVGCPVCRR
                     EFEIIEGIVDFTAAASGEHPGRTVRRTPAAPSPVALEPQSLQALLDLSGPGGYVILLG
                     SAARSAVGLAGLMGGVHFVGINAPPDVEELPVLSLVQSDRVIPLRGAMARAAVVGAEL
                     AGTPWVGEAVRVLLPGRRLVVESATAALPEQVVQLAAGQGLWVGEKR"
     gene            complement(1831..>3422)
                     /locus_tag="AC16JC_02910"
     CDS             complement(1831..>3422)
                     /locus_tag="AC16JC_02910"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR5163677.1"
                     /translation="YACLCARAADVPIYPTLTARQTEYILKDSGSVAVFCSTVEQVAK
                     VVEIRGNLPALRHVIVFDAGGKREGVVTLDQLEAKGRGVAAAHPHVREEALAVSPHAI
                     ATLIYTSGTTGDPKGVILTHNNIWSNVQGGLQALPLLPTDECLAMLPLSHVYERMVDY
                     TLLSAGVVINYAESFDKVAQNLAEVRPTIVLSVPRLYEKVYARVLENALSGSALKRRI
                     FLWAKRAGEAWATLVLDQQPIPAGLALKHRIADRLVFAKLRARTGGRIRYFISGSAPL
                     SPEIAKFFFSAGLPVLEGYGLTETSPVLTLNPYRRPRIGTVGVTFPDVQLKIAADGEI
                     LAKGPNVMQGYYNKPEATAEAIDAEGWFHTGDIGELDADGYLKITDRKKDLIKTAGGK
                     FIAPQPIENQAKLNKFVANAVLLGDKRKFSIILVVPNFETLEPWATAQGLTFTSRRQL
                     IALPAVQQKMDDEVGSTMRDLAHFERPKKIVLLEQDFTIESGELTPTLKVKRRVVEKN
                     YAPVIDAAYAERDSLAASVEG"
BASE COUNT          513 a         1167 c         1168 g          574 t
ORIGIN      
        1 acgcgcgatt cggtcatcgg tcgaattcag agccagtacg tgctcgccat cgtcgcggat
       61 acgagcccga acatgtggat cagcacgctc aaggggatcg gccattcccc cgacgccgga
      121 cgcacctggc acgaacattt cccgccggcg ccgtgcacag gcgtgggatg tgtcaatcgc
      181 gctcgagcgc tgtcattgga ctcgggcggc aggttctgga tcgggaccga gcaggggttg
      241 ttccgattcg ggccggaatt cggcggctgg gcgacgcctg acgggcaacc gtgcggcaag
      301 cgcgcgtcgt gtccgggcgg gccgctcccc gtccgggcct tcacttcccg caccctccgc
      361 ccaccgggcg aggcaccccg gtcgtcgccg ccggagaagc cgcgccacac ctggttccgc
      421 cgccccatcg cgctcgcgga ccagccctac atcgaccaga cgtaccgctt cggctcgacg
      481 atgggcggca acttccagcc gcaccagggc gtcgagttca acaaccccga cggcacgccg
      541 gtgcacgcca tcggcgacgg cgtcgtcgtc tactcggggc ctgccgaagc gggagcgaac
      601 accgtcgcaa tccgccatga ccgaccactg aaaaccgacg acggacgacg ccagtacgtc
      661 ttctccgtct actaccacaa cacgaagctt gtcgccacgg tcgggcagcg cgtgaaagcg
      721 ggcgacgtca tttccctggt gggcaatacg ggacgggcca cgaacgatca tctgcacctc
      781 gaggtgcacg tggcccccac caccgattcg agcaagatcg tggacgccgc cgttcgctac
      841 ccgccgttca acaccaaccc cgagctctgg atcgatccgt tgcccgggac gggcctggtc
      901 gccgggcagg tgtgggactc gcacggcgag cccgtccagc aggcgcggat ctacgggctc
      961 gtcaagcccg agccgcagga gacgccgttc tcgttcatcg agacctatgg ggcgcgcaat
     1021 cactccgatc cggtgtatca cgagcatttc gccatcggcg acgtgccgcc gggtgaatac
     1081 gacctcggcg tggaaatcga gggaagaaag gtctaccggc gggttcgtgt tacggcagga
     1141 aagctgacct gggtcgaatt ccatccctga gcgacccttc cctcttccct catccctctt
     1201 cccccgcctc atgcacatcg agctcaccga gatgctgcgc tgccccgagg atcatcgcga
     1261 ggagttcctc gtcctctcga cctcggagat gaacggccgc atggtgatgc gcggggtggt
     1321 ggggtgcccg gtgtgccggc gggaattcga gatcatcgag ggaatcgtgg atttcaccgc
     1381 ggccgcgagc ggcgagcatc cggggcgcac cgtgcggcgc acgccggcgg cgccttcccc
     1441 cgtcgccctc gaaccccagt cgctgcaggc gctgctggac ctcagtggac cgggcggcta
     1501 cgtgattctc ctgggctcgg cggcgcgctc cgccgtcggt ctcgcgggcc tgatgggcgg
     1561 ggtccatttc gtcggcatca acgcgccgcc cgacgtggag gagctcccgg tgttgagcct
     1621 ggtgcaatcc gaccgggtga tcccgctgcg cggcgccatg gcgcgtgcgg ccgtcgtcgg
     1681 cgcggagctg gccggaacgc cgtgggtcgg ggaagcggtc cgcgtgctgc tccccgggcg
     1741 gcggctggtg gtcgagtcgg cgacggcggc gctgcccgag caggtcgtgc aactggcggc
     1801 cgggcagggc ctgtgggtgg gggagaagcg ctagccctcg acgctcgccg ccagggaatc
     1861 gcgctccgcg taggccgcat cgatgaccgg tgcgtagttc ttctccacca cgcggcgctt
     1921 cactttgagg gtcggggtga gctcacccga ttcgattgtg aagtcctgct cgagcagcac
     1981 gatcttcttg gggcgctcga agtgggccag gtccctcatc gtgctcccca cttcatcgtc
     2041 catcttctgc tggacggccg ggagtgcgat gagctggcgg cgggacgtga acgtcaaccc
     2101 ctgcgccgtg gcccacggct ccagcgtctc gaagttcggc acgacgagga tgatcgagaa
     2161 cttgcgcttg tcgcccagca gcacggcgtt ggcgacgaac ttgttgagct tggcctggtt
     2221 ctcgatcggc tgcggggcga tgaatttccc ccccgccgtc ttgatgaggt ccttcttgcg
     2281 gtcggtgatc ttgaggtagc cgtcggcgtc cagctcgccg atatcgccgg tgtggaacca
     2341 gccttcggcg tcgatcgcct cggccgtcgc ctcgggcttg ttgtagtagc cctgcatgac
     2401 gttcggtccc ttggcgagga tctcgccgtc ggcggcgatc ttgagctgca cgtcggggaa
     2461 cgtcacgccc acggtgccga tgcgcggccg ccggtacggg ttgagcgtga gcacggggga
     2521 ggtctcggtg agcccgtagc cctccagcac cggcagcccg gcgctgaaga agaacttggc
     2581 gatctcggga gagagcgggg cgctgcccga gatgaagtag cggatccggc ccccggtgcg
     2641 ggcccgcagc ttcgcgaaca ccagccggtc ggcgatcctg tgcttgagcg cgagcccggc
     2701 gggaatcggc tgctggtcga gcaccagggt cgcccacgct tccccggcgc gcttcgccca
     2761 gaggaagatg cggcgcttca gggcgctccc cgagagcgcg ttctcgagca cccgcgcgta
     2821 caccttctcg tacagccgcg gcacgctgag cacgatggtc ggccgcactt ccgcgagatt
     2881 ctgggcaacc ttgtcgaacg actcggcgta gttgatcacc acgccggcgg acagcagcgt
     2941 gtagtccacc atccgctcgt agacgtgcga cagcgggagc atcgccaggc actcgtcggt
     3001 gggcagcagc ggcagggctt gcaggccccc ctgcacgttc gaccagatgt tgttgtgggt
     3061 gaggatcacg cccttgggat cgcccgtcgt cccggacgtg tagatcaacg tggcgatcgc
     3121 gtggggcgat acggcgagcg cctcctcgcg cacgtgcggg tgcgccgcgg cgacgccccg
     3181 ccccttggcc tccagctggt cgagcgtgac cactccctcc cgcttgccgc cggcgtcgaa
     3241 cacgatcacg tgccgcagcg cggggaggtt gccccggatc tccaccacct tcgctacctg
     3301 ctccacggtc gagcagaaca cggcgaccga tcccgagtcc ttcaggatgt actccgtctg
     3361 ccgcgcggtc agggtcgggt agatcgggac gtcggccgcc cgcgcgcaga ggcaggcgta
     3421 gt
//
LOCUS       DBNGZW010000119         4947 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_896199, whole genome shotgun sequence.
ACCESSION   DBNGZW010000119 DBNGZW010000000
VERSION     DBNGZW010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4947
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_896199"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="AC16JC_02915"
     CDS             <1..391
                     /locus_tag="AC16JC_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163678.1"
                     /translation="GVAYHPALRADLGRSDFADPGLRARIRDVRSQLLDRALTLAPAY
                     ERMMRALPFRAVSPARGSGALMLLFTDRYYRSCARLVGRPTWPGPMSTTKLIFLSACH
                     AIMKRWSARSTRRITENLARHARASSA"
     gene            494..1513
                     /locus_tag="AC16JC_02920"
     CDS             494..1513
                     /locus_tag="AC16JC_02920"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR5163679.1"
                     /translation="MKTRLSAAALMVLAIAACRSRAPEASVVLIDSARLAQFAPLPDS
                     MATPDNPITLAKADLGRMLFYETRLSRDHDLSCNSCHDLAQYGVDHAPVSVGAQGQLG
                     KRNAPTVYNAAGHIAQFWDGRARDVEEQAKGPILNPVEMAMPSDRALLLELAALPAYR
                     EAFRRAFPGEANPITYDNVGRAIGAFERQLVTPSRWDALLKGDRNALTAPEKQGFNDF
                     VEAGCVSCHSGAYLGGQMYQKAGLVTPWPDRSDPGRIAVTRKAADSMVFKVPSLRNIA
                     MTGPYFHNGATASLEDAVVMMGHYQLGRDLTATQVQSIVTYLRALTGTLPQRSIVPTP
                     PVPEH"
     gene            complement(1562..3595)
                     /locus_tag="AC16JC_02925"
     CDS             complement(1562..3595)
                     /locus_tag="AC16JC_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5163680.1"
                     /translation="MTRPAPQSLTRLLLLTALITTTAAAALAAQAPARALTAGDLVYR
                     LRAVADPQLSPDGNWVAYTVTTTDSAKNRRDADVWMTSWDGTQTLRMTATPERETTPR
                     WSPDGRYLAFLSGREDGHGNQVWLLDRRGGEAQRLTTIKGGVDAMAWSPDAKRVVLVA
                     TVDLDTTKADTTKKRPIVIDRYRYKQDVAGYLTTERSHLLLVDLATKRVDTLTAGSAD
                     DASPSWSPDGTRIAFVRTPPPEPGTGDESDVWVVEARRGAEPRALTAFAGPDNGPLAW
                     SPDGVWIAFFRTDDPKYYAYQLQKLAIVWADGSTPARILSAAFDRPVDAPTFTADGRS
                     VLVLADDDRVTGLTRVRVADGRVERVDQGKHVVLRYSAGALAGARVALLLSTPDRPAE
                     VFASEGAQVRQLSHQNDSLLATVRLSPTEDFVSHSADGTEVHGVLVRPAGTAAGTRLP
                     LILWIHGGPNGQDDYSFSQNRQLFAANGYAVLSVNYRGSDGRGAAFQRAIFADWGHHE
                     VEDLLGAVDEAVRSGIADPDRLGIGGWSYGGILTDYTIATTTRFKAAISGAGSALQLA
                     IYGTDQYITQYELELGPPWQARDLWIKLSYPFFSADRIKTPTLFMGGQADFNVPIIGG
                     EQMYQALRELGVPTQLVVYPGQYHGLTDPSFLKDRFDRWVAWFDRYVKGERTK"
     gene            3672..4532
                     /locus_tag="AC16JC_02930"
     CDS             3672..4532
                     /locus_tag="AC16JC_02930"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5163681.1"
                     /translation="MSDTHEISHVSDTAHWVAVYRAMETERPDAIFRDPYARRLAGER
                     GEAIVRALPRGRNWDWPMIVRTAVMDEILLRAIARDGVRTVVNLAAGLDARAYRLELP
                     HALRWIDVDLPAIQDSKRDALSGETPRCALEWVPADLADAAQRRGVLQRIAADQGPAL
                     AITEGLLIYLQPDQVAELARDLHDCPTLSWWLMDLASPRLLKLMARTWGKSVERGAPF
                     RFAPAEGTAFFAPAGWREAEFRSMWDESLRLKRTMPFAGFWNLVGKLYPKKTRQEMRR
                     MSGIVLMKRS"
     gene            complement(4650..>4947)
                     /locus_tag="AC16JC_02935"
     CDS             complement(4650..>4947)
                     /locus_tag="AC16JC_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163682.1"
                     /translation="GQRATSPADRNCANGTVLKRNTLRKDNAFFSWDVRISHSFPVGR
                     PGQALETIVEIFNVTGNDNFKDPAYGGLLFNFDGTIRSGLGDPRQLQVGARWVF"
BASE COUNT          733 a         1749 c         1688 g          777 t
ORIGIN      
        1 gggcgtggcc tatcaccctg ccctgcgtgc ggacctcggc cggtcggact tcgcagaccc
       61 ggggctgcgc gcgcgcatac gcgacgtccg gtcgcagctg ctcgaccgcg ccctcaccct
      121 cgctcccgcc tacgagcgga tgatgcgggc gctgccgttc cgcgcggtga gtccggcgcg
      181 cggctccggc gccctgatgc tgctcttcac cgaccgctac taccggtcgt gcgcccgcct
      241 cgtggggcgc ccgacctggc ccgggcccat gtccaccacg aagctgatct tcctgtcggc
      301 ctgccacgcg atcatgaagc gctggtccgc caggtccacc cgacgcatca ccgagaacct
      361 ggcgcgccac gcacgggcca gctccgccta gcccccggta cgcgaggggt cttccgcgcc
      421 gattcacggt cgtttcacgg cctcctcatc actttgggac tgatcgcaac gaatctccgc
      481 ggaggccacg aacatgaaga cccgtctctc ggcagcggcg ctgatggtgt tggccatcgc
      541 cgcgtgccga tcacgcgcac cagaggcgtc cgtcgtgctc atcgactcgg cccggctcgc
      601 gcagttcgcg ccgctgcccg actcgatggc gacgccggac aatccgatca cgctggccaa
      661 ggccgacctt ggccgcatgc tgttctacga gaccagactc tcgcgcgacc acgacctctc
      721 gtgcaacagc tgtcacgatc tcgcgcagta cggggtggat catgctccgg tgagcgtcgg
      781 ggcgcagggt cagctcggca agcgcaatgc gccgacggtc tacaatgccg ccgggcacat
      841 cgcgcagttc tgggacggcc gggcccggga cgtcgaggag caggccaagg ggccgatcct
      901 caacccggtc gaaatggcga tgccgagcga tcgcgcgctg ctgctcgagc tcgcggcgtt
      961 gcctgcctat cgtgaggcgt tccgccgtgc gttcccgggc gaggcgaacc cgatcaccta
     1021 cgacaacgtg ggccgggcga tcggcgcctt cgagcggcag ctcgtgacgc cgtcgcgctg
     1081 ggacgccctg ctgaaagggg accgcaacgc gctgacggcg cccgagaagc aggggttcaa
     1141 cgacttcgtc gaggccgggt gcgtgagctg ccacagcggc gcgtatctgg gcgggcagat
     1201 gtaccagaag gcgggactcg tgacaccctg gcccgatcgc tccgatccgg gccgcatcgc
     1261 cgtgacccgc aaggccgccg attcaatggt gttcaaggtg ccctcgctgc gcaacatcgc
     1321 gatgacgggg ccgtacttcc acaacggcgc caccgcctcg ctcgaagacg cggtggtgat
     1381 gatggggcac taccagctcg gccgcgacct gacggccacg caagtgcagt cgatcgtaac
     1441 gtacctgcgc gcgctgacgg ggacgctgcc gcagcgttcc atcgtcccga cgccacccgt
     1501 gcccgagcac tgatcgcgcg ctgggtgaca ccccagggcc cggccgcgcc gcggtcgggc
     1561 cctatttcgt ccgctccccc ttcacgtatc ggtcgaacca cgccacccag cgatcgaacc
     1621 gatccttgag aaacgacggg tcggtcaacc cgtggtattg accaggatac acgacgagct
     1681 gcgtcggcac gcccagctcc cgcagcgcct gatacatctg ctccccaccg atgatgggaa
     1741 cgttgaagtc ggcctgcccc cccatgaaca gcgtgggcgt cttgatccgg tccgcactga
     1801 agaacggata ggagagcttg atccacaggt cccgggcctg ccacggcggg cccagctcca
     1861 gctcatactg cgtgatgtac tgatcggtgc cgtagatcgc cagctgcaag gcgctgccgg
     1921 caccgctgat ggccgccttg aagcgggtcg tggtggcgat cgtgtagtcg gtgaggatcc
     1981 cgccgtaact ccagccgccg atgccgaggc gatccggatc ggcgattccg gaacgcaccg
     2041 cttcgtccac ggcacccagg agatcttcga cttcatggtg gccccagtcg gcgaagatcg
     2101 cccgctgaaa cgccgcccca cgcccgtcgc tgccccggta gttcaccgac agcacggcgt
     2161 agccgttggc cgcgaagagc tggcgattct ggctgaacga gtagtcgtcc tggccgttcg
     2221 gcccgccgtg gatccacagg atcaacggca gccgtgtccc ggccgccgtt cccgcgggac
     2281 gcacgagcac gccgtgcacc tcggtgccgt cggcgctgtg cgaaacgaaa tcctcggtag
     2341 gcgacaggcg caccgtggcg agcagagagt cgttctggtg cgacagctgg cgtacttgtg
     2401 ccccctcgct ggcgaagact tcagccggcc gatccggcgt gctgagcagc aacgcgaccc
     2461 gcgcaccggc cagggcgccc gccgagtaac gcagcaccac gtgtttcccc tgatccacgc
     2521 gctccacccg cccgtcggcg acgcgcacgc gggtgagccc ggtgacgcgg tcgtcgtcgg
     2581 cgaggaccag gaccgagcgg ccgtcggcgg tgaaggtggg cgcatcgacc ggccggtcga
     2641 acgcggcgga gaggatgcgc gccggtgtcg agccgtcggc ccacacgatc gcgagcttct
     2701 gcagctggta agcgtagtac ttcggatcgt cggtccggaa gaaggcgatc cacacgccgt
     2761 ccgggctcca ggcgaggggg ccgttatcgg gcccggcgaa cgcggtcagc gcccgcggct
     2821 ccgccccgcg ccgcgcctcc accacccaca cgtcggactc atcgccggtc cccggctcgg
     2881 ggggcggcgt gcgaacgaag gcgatgcggg ttccgtcagg ggaccagctg ggactggcat
     2941 cgtcggcgct gcccgccgtg agggtgtcta cccgcttcgt ggcgaggtcc accagcagca
     3001 agtggctgcg ctcggtcgtg aggtacccgg cgacgtcctg cttgtagcga tagcggtcga
     3061 tgacgatagg ccgtttcttc gtggtgtcgg ctttcgtcgt gtcgaggtcc accgtggcca
     3121 cgaggacgac ccgtttggcg tccggcgacc acgccatggc gtccacgccg cccttgatcg
     3181 tcgtcagccg ctgggcctcc ccgccgcgcc ggtcgagcag ccacacctga ttgccgtgcc
     3241 cgtcctcgcg tcccgacagg aaggcgaggt agcgtccatc gggactccag cgcggcgtcg
     3301 tctcgcgctc gggagtggcg gtcatgcgca gggtctgtgt cccgtcccag ctcgtcatcc
     3361 agacatcggc gtcgcggcgg ttcttcgccg aatccgtggt ggtgacggta taggcgaccc
     3421 aattgccgtc gggggagagc tgcgggtcgg ccacggcccg caggcgatac accaggtcgc
     3481 ccgccgtcag cgccctggcg ggggcctggg cggcgagcgc cgcagcggcg gtcgtggtga
     3541 tgagtgccgt gagcaggagc aaacgagtca gggactgcgg agcgggtcgc gtcattgggc
     3601 gatacgatat cgtcggccgt gcccgacggc tacgccttcg cggcgtgtcc tcccgatgct
     3661 aggtttgccg catgtccgac acccatgaga tctcgcacgt ctccgacacg gcccactggg
     3721 tcgcggtgta ccgcgccatg gagaccgagc gtcccgacgc catcttccgc gacccctacg
     3781 cccggcgcct cgcgggcgag cggggtgagg cgatcgtccg ggcgttgccg cgcggccgga
     3841 actgggactg gccgatgatc gtgcggaccg cggtgatgga cgagatcctg ctgcgtgcca
     3901 tcgcccgcga cggcgtgcgc acggtggtga atctggccgc ggggctcgac gcccgcgctt
     3961 accgcctcga gctgccccac gcgctgcgct ggatcgacgt ggacctcccg gcaatccagg
     4021 actccaagcg cgacgcgctg tcgggcgaaa cgccgcgctg cgcgctggag tgggtgcccg
     4081 cggacctggc cgatgccgcg cagcgccgcg gcgtgttgca gcggatcgcg gccgaccagg
     4141 gtccggcgct cgccatcacc gagggactgc tcatctatct gcagcccgac caggtcgcgg
     4201 agctcgcgcg cgatctgcac gactgcccca ccctctcctg gtggctcatg gacctcgcca
     4261 gcccgcggct gctcaagctg atggcgcgca cctgggggaa atccgtggaa cgcggcgcgc
     4321 cgttccgctt cgcgccggcc gaggggacgg ctttcttcgc gccggcgggg tggcgggaag
     4381 cggagttccg ctcgatgtgg gacgagtcgc tgcggctcaa gcgcaccatg ccattcgccg
     4441 gcttctggaa cctggtggga aagctgtacc cgaagaagac gcgccaggag atgcggcgga
     4501 tgtcggggat cgtcctgatg aagcgatcgt aggggacggt aggggacggg aggggttagg
     4561 cgatcccacc cacgtctccc gcaatccccc ttccatcccc tacctcccct ttcgtcccct
     4621 accgtcccct accgtccccc taccgccccc tagaacaccc accgcgcgcc gacctgcagc
     4681 tgccgcgggt cgcccagccc cgagcggatg gtcccgtcga agttgaacag caggccgccg
     4741 taggcggggt ccttgaagtt gtcgtttccg gtcacgttga aaatctccac gatcgtttcg
     4801 agcgcctggc ccgggcggcc gaccgggaac gagtgcgaga tccggacatc ccacgagaag
     4861 aatgcgttgt ccttgcgcag cgtgttgcgc ttcagcacgg tgccgttggc gcagttgcgg
     4921 tcggccgggc tggtggcccg ctggccg
//
LOCUS       DBNGZW010000120        10385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_896236, whole genome shotgun sequence.
ACCESSION   DBNGZW010000120 DBNGZW010000000
VERSION     DBNGZW010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 10385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10385
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_896236"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="AC16JC_02940"
     CDS             <1..363
                     /locus_tag="AC16JC_02940"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013423659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5163683.1"
                     /translation="NQIWANLVDNALDAVGTAGHVAVTARRQGDRVVVEVADDGPGIP
                     PEIRERIFEPFFSTKDVGKGTGLGLDIVRRTLERHDGEIDVESRPGRTVFRVKLPAQG
                     MRSSGRWSRSTSRIQIEE"
     gene            complement(365..2026)
                     /locus_tag="AC16JC_02945"
     CDS             complement(365..2026)
                     /locus_tag="AC16JC_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012407375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5163684.1"
                     /translation="MAKPVIMSVDDDPEVLGAVERDLRRHFRSDYRILAVSSGPQALE
                     TTQQLKARDAAVALFLVDERMPQMTGTQFLTEARRLYPDARKVLLTAYADTEAAITGI
                     NAVGLDHYLMKPWDPPEQHLYPVLDELLADWTAGFRVPFEGIRVAGSRWSPRSYEIKD
                     FLSRNQTPYRWIDLDADPATRELVETITPGLKQLPVVFFPDGTTLAAPTSRELAERIG
                     LQTQARQPFYDLIVVGGGPSGLAAAVYGASEGLRTILLEVGATGGQAGTSSQIENYLG
                     FPSGIAGADLARRATIQARRFGAEVVTPRGVAAIRVEDPYRIVVLDDGTELRCYALLL
                     ATGMIVRRLDVPGADALTGAGVYYGAAVAEATAHRGADVIVVGGANSAGQAALLFARY
                     ARKVTMLVRGPSLQASMSTYLIDRIAAAENIEVRTHTSVVAVCGTDRLESVVLQHAET
                     GERATLPATAMFVFIGSAPLSDMVAGLVERDELGFVITGPDLLHGGKRPKGWTLDRDP
                     FLLETSVPGIFASGDVRLGSTKRVASAVGEGSAAVGMIHKYLETV"
     gene            complement(2019..3626)
                     /locus_tag="AC16JC_02950"
     CDS             complement(2019..3626)
                     /locus_tag="AC16JC_02950"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5163685.1"
                     /translation="MTTSLGWLERISYSPGDDEETRRRKVQFTIASILVIPAGLVWGA
                     LYFAFGERTVAAIPAAYSVLTLLDLLVLSRLRRYELFRQTQQFLILILPFALQLALGG
                     FVGSSLVILWSFIAVLMALLFGGVRETVWWFVAYLTAVVAAALLQPRLAIDNPLPTQL
                     VLAFFVLNVGTVSSIAFVVLRSFVTDRRRLRELEVAYLNQEMMLRQSERLATLGTLAA
                     GVAHELNNPAAATRRAAEQLGDAFARLETAHLRLNAVTLTSAGRDLLRSIERQGREQA
                     VRPNGLDVLARSDEEAVVEEWLEARGIADPWQLAPALVGQGLDRPALTRLAATLQGEA
                     LDAALAWAASVFSVYTLLYEIGQGSTRVAEVVGALKSYSYLGEAPVQSVDLKEGLDNT
                     LVILRNKLKAGITVHRDYGADVPPVQAYGSELNQVWTNLLDNAADAMDGRGEITIRTR
                     RRDGWAVVEIEDNGPGIPAEIQPRIFDPFFTTKAPGKGTGLGLSTSYTIVTRKHAGEI
                     RVTSRPGFTCFTVKLPIEGPSTEAQHG"
     gene            complement(3793..4704)
                     /locus_tag="AC16JC_02955"
     CDS             complement(3793..4704)
                     /locus_tag="AC16JC_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5163686.1"
                     /translation="MRSTPLRATTMTQNALESHDWRPLTAALVSVTLWSSAFVGIRAA
                     TRTIPPGALALGRLTIGSVLLGLLLWRRGGLRPTRRELGLLLVAGLLWFGFYNVALNT
                     SERVVDAGTAAMVVNIAPLMIMALAALFLRERLTSRLLAGGAVAFGGVIVIGLATMSG
                     TATPWGVLLSVLATTASAGGVVAQKPVLTRLSALQVTWTCCAIGALCCLPYAPSLGRA
                     VHTASSVDLAWMVYLGVFPTSVAFTTWAYALARGTASRLAATAYLVPPITIGMSWLIL
                     GEVPVAVAILGGALCVAGVVVARRRPS"
     gene            complement(4697..5338)
                     /locus_tag="AC16JC_02960"
     CDS             complement(4697..5338)
                     /locus_tag="AC16JC_02960"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018613678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR5163687.1"
                     /translation="MNVGIIGSGPVAQSLGAGFLKHGYAVMLGTRDAAKLAQWRAQHA
                     GARIGSFADAAAFGEIVVLAVKGDVAGDALRLAGPTALAGKVVVDTTNPIAAAPPVNG
                     VLKFFTSLDDSLMERLQQASPDVRFVKAFNSVGNAKMVNPQYAAGRPTMFICGNDTAA
                     KRIVSGVLDQFGWETLDLGAVEAARAIEPLCILWCIPGFLHNEWTHAFKVLHA"
     gene            complement(5343..5993)
                     /locus_tag="AC16JC_02965"
     CDS             complement(5343..5993)
                     /locus_tag="AC16JC_02965"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013692837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0052851 - ferric-chelate reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HIR5163688.1"
                     /translation="MRIGILGSGLMGAKLGTIWTRAGHEVVFSYARSAQKLQRLAREA
                     RGKARAGTPGEAAREADALLLAVHWSRVDDVVRQAGDLSGKVIVTCSLPMTADDTDLV
                     IAHTSSGAEALAKRIAGARVVSAFGTVPSEVLFGVFEARRKATKPSLVYCGDDARSKR
                     IAAQLIRDVGFDPVDAGPLRIARYIEPFTLLIAQLAYEGDGGPELAYRFERFRRKG"
     gene            complement(6009..7004)
                     /locus_tag="AC16JC_02970"
     CDS             complement(6009..7004)
                     /locus_tag="AC16JC_02970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007789779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5163689.1"
                     /translation="MQKRTLGTSNLEVSAIGLGCMGMSFGYGPAADRREMIALIRAAV
                     EGGVTFFDTAEAYGPFTNEELVGDALAPFRGQVVIATKFGWDIDPVTGQHHGGVNSRP
                     EHIRTAVEGMLRRLKCECIDLLYQHRVDPNVPIEDVAGTVQDLIREGKAKHFGLSEAG
                     AQTIRRAHAVQPIAALQSEYSLWWREPEAEILPTVEALQIGFVPFSPLGKGFLTGSID
                     ATTTFDRSDFRNTVPRFAAENRMANQSMVDLVVRVAARKRATPAQIALAWLLARKPWI
                     VPIPGTTKRHRLDENLGATARDLTPDDLQEIESAAATITVHGERYAESSQRFIDR"
     gene            7117..7188
                     /locus_tag="AC16JC_02975"
     tRNA            7117..7188
                     /locus_tag="AC16JC_02975"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7149..7151,aa:Thr,seq:cgt)
     gene            complement(7195..7890)
                     /locus_tag="AC16JC_02980"
     CDS             complement(7195..7890)
                     /locus_tag="AC16JC_02980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163690.1"
                     /translation="MPWSILALACTSPSQQAMQGLFWAGPADGGPWLIYLKPGSDSSA
                     AYLTPHTLAVCDLRVFGGGEVSFRTSSTGDGAAYRFVGHWSASGLTGSMRQARTQPGT
                     LIDTPQLDLRRIAATLVSASESISGAYTEASPRRRSSVELVLVDAPGGLVRVLVDHDG
                     GAAGIRGVTGVRDGNVLRLSLQSPTRSETDAAHIRGDTLILHGSRLRLVKQSTLPALL
                     FGPNRKECDEGPR"
     gene            complement(8008..9345)
                     /gene="preA"
                     /locus_tag="AC16JC_02985"
     CDS             complement(8008..9345)
                     /gene="preA"
                     /locus_tag="AC16JC_02985"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013589009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HIR5163691.1"
                     /translation="MADLHTTFAGIESPNPFWLASGPPTNTYGQVARAFDLGWGGAVW
                     KTVGEPIINVFSRYGSVDLGSSRMMGFNNIELITDRPVADNLKEIAAVKRNYPRHAVI
                     VSLMVESKRGVWHDMVQRTQDTGADGIELNFGCPHGMSERGMGSAVGQVPDYTCQIVE
                     WVKEVAAIPVIVKLTPNVTDITYIARAAVKGGADALSLINTINSIVGVDLDRFAPLPD
                     VGGRSSHGGYCGPAVKPVALHLVSAVAGDPAVRVPISGIGGIATWRDAAEFVALGATT
                     LQVCTAVMHHGFRIIEDLVDGLTMWMDGAGHRTLDDVRGRALPNVVKWEDLDLNYHLV
                     AEIDQATCIGCRLCFVACEDGAHQAIRTLGTRDQRRGTGDGGRGTGGPVVEIIEEACV
                     GCNLCSEVCPVQGCITMRRIPNDCGIVSWKDYSSGRGKLAPRSAQFHTAVWGT"
     gene            complement(9356..>10385)
                     /locus_tag="AC16JC_02990"
     CDS             complement(9356..>10385)
                     /locus_tag="AC16JC_02990"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5163692.1"
                     /translation="PFGHSCARACPVEVLCEGACVLIQRDERPIKIALLQRHATDHVL
                     AHHLRLFEPGPPSGKRVAIVGAGPAGLSCARDLVRRGHAVTVFEAQDRPGGLNTFGIA
                     EYKMTPAVALAEVADILYLGIELRTGVMIGRDVSFDALLADYDAVFVGVGLGLTNRLG
                     IPGESLAGVVDALTFIAHLKTHPYRETRVGRRVVVIGAGNTAIDAVTQAKRLGASDAT
                     IVYRRGETDMPCFDYEYELAQRDGCVFRFHCTPRRILGVHHVEGLEVEHAGEIEVLPC
                     DMIITALGQAARDEFLLPSHPKVFVGGDYANGGAEIVNAAAEGVRAAAKIDEMLAEEG
                     GRGRKKTE"
BASE COUNT         1707 a         3597 c         3481 g         1600 t
ORIGIN      
        1 aatcagatct gggccaacct ggtggacaac gcgctcgatg ccgtcggcac ggccgggcac
       61 gtggcggtca cggcccggcg gcaaggggac cgggtggtcg tcgaggtcgc cgacgacggt
      121 cccggcattc cgcccgagat ccgcgagcga atcttcgagc cgttcttctc caccaaggac
      181 gtggggaagg gaaccgggct cggcctggat atcgtgcggc ggacgctgga gcggcacgac
      241 ggagagatcg acgtggaatc gcgcccgggc cgaacggtgt tccgggtcaa actcccggcc
      301 cagggcatgc ggtccagcgg gcgctggtcg cggtcgactt cgcgaattca gatcgaggag
      361 tagctcacac cgtttccaag tacttgtgga tcatccccac ggcggccgat ccctccccca
      421 ccgccgaagc cacgcgtttg gtcgagccga ggcggacgtc gccggaggcg aagatgccgg
      481 ggacgctcgt ctcgagcagg aacggatcgc ggtcgagggt ccaacccttg ggccgcttgc
      541 cgccgtgcag caggtccgga ccggtgatga cgaagccgag ctcgtcgcgc tccaccaggc
      601 cggccaccat gtccgacagc ggtgccgagc cgatgaagac gaacatcgcc gtcgccggga
      661 gggtggcgcg ctcacccgtc tcggcgtgct gcagcacgac cgattcgagc cgatcggtcc
      721 cgcagacggc gacgacggag gtgtgcgtcc ggacctcgat gttctccgcg gccgcgatgc
      781 ggtcgatgag ataggtcgac atggacgcct gcagcgacgg cccgcgcacg agcatggtga
      841 ctttccgggc gtagcgggcg aacagcagcg ccgcctgacc ggcggaattc gctcccccga
      901 ccacgatcac gtccgcgccg cgatgggcgg tcgcttcggc gaccgccgcg ccgtagtaca
      961 cgcctgcccc ggtgagcgcg tccgcccccg gcacgtcgag gcgccgcacg atcatcccgg
     1021 tcgcgagcag cagggcgtaa caccggagct cggtaccatc gtccagcacg acgatgcggt
     1081 acgggtcctc cacccggatc gcggccacgc ccctcggtgt caccacctcg gcgccgaagc
     1141 gccgggcctg gatggtggcg cgccgcgcca ggtcggcgcc ggcgatgccg ctcgggaagc
     1201 cgagatagtt ctcgatttgc gagctggtac cggcctgccc gcccgtcgcg ccgacttcga
     1261 gcaggatggt ccgcaatccc tcggacgcac cgtacacggc cgccgccaag ccgctcggac
     1321 cgccgccgac cacgatgagg tcgtagaacg gctgtcgcgc ctgggtctgc aaaccgatcc
     1381 gctcggccag ctcgcggctc gtcggggcgg ccagcgtcgt gccgtcggga aagaagacca
     1441 cgggaagctg cttgagcccc ggcgtgatcg tctcgaccag ctcgcgcgtc gccgggtcgg
     1501 cgtcgagatc gatccagcgg tacggggtct ggttccggga gaggaagtcc ttgatctcgt
     1561 agctccgcgg cgaccagcgg gacccggcga cccggatccc ttcgaacggc acgcggaagc
     1621 ccgcggtcca gtcggccagc agctcgtcca gcacgggata gagatgctgc tccggcggat
     1681 cccacggctt catcagatag tggtccaggc cgacggcgtt gatgcccgtg atcgccgcct
     1741 ccgtgtcggc gtaggccgtc agcagcacct tgcgggcatc cggatacaac cggcgcgcct
     1801 cggtgaggaa ctgggtgccg gtcatctgcg gcatgcgctc gtccacgaga aacaacgcga
     1861 cggccgcgtc ccgcgccttg agctgctgcg tggtctccag cgcctgggga cccgagctca
     1921 ccgcgaggat ccggtagtcg gaccggaaat gccggcgcag gtcgcgctcg accgcgccga
     1981 gcacctcggg gtcgtcgtcg acgctcatga tgacgggttt agccatgctg tgcctccgtg
     2041 gacggcccct cgatcggcag cttcacggtg aaacaggtga agccgggccg tgaggtaacg
     2101 cgaatctcgc ccgcgtgctt ccgggtaacg atagtgtagc tggtcgacag ccccaggccc
     2161 gtccccttcc cgggcgcctt ggtcgtgaag aacggatcga agatccgggg ctggatctcc
     2221 gccgggatgc ccggcccatt gtcctcgatc tcgacgaccg cccagccgtc gcggcggcgc
     2281 gtgcggatgg tgatctcgcc ccggccgtcc atcgcatcgg ccgcgttgtc gagcaggttg
     2341 gtccacacct gattcagctc gctgccgtac gcctgcaccg ggggaacgtc ggcgccgtag
     2401 tcgcggtgca ccgtgatgcc tgccttgagc ttgttgcgca ggatcaccag ggtgttgtcg
     2461 agaccttcct tcaggtccac cgactgcacc ggcgcctccc cgagatagga gtagctcttg
     2521 agcgcgccga cgacctcggc gacgcgcgtc gagccctgtc cgatctcgta caggagcgtg
     2581 tagacggaga acacgctggc ggcccaggcg agggcggcgt cgagcgcttc gccctggagg
     2641 gtggcggcga gccgtgtgag tgcgggccga tcgagcccct gaccgaccag tgcgggagcg
     2701 agctgccacg gatccgcgat gccgcgcgct tcgagccatt cctcgacgac tgcttcctcg
     2761 tcggaacgcg cgagcacatc cagaccgttc gggcgcacgg cctgctcgcg cccctgccgc
     2821 tcgatcgacc gcagcaggtc ccgtccggcg gaggtgagcg tcaccgcgtt caggcggagg
     2881 tgggcggtct cgagacgggc gaacgcatcg ccgagctgct cggcggcccg gcgggtagcg
     2941 gcagcggggt tgttcagctc gtgcgccacc cccgcggcga gcgtgcccag cgtggccagc
     3001 cgctccgact ggcgcagcat catctcctga ttgaggtacg ccacctcgag ctcccggagg
     3061 cgccgtcgat ccgtcacgaa ggagcgcagc acgacgaagg cgatcgacga gaccgtgccg
     3121 acgttgagca cgaagaaggc gaggacgagc tgggtcggca gggggttgtc gatcgccagg
     3181 cggggctgca acagcgcggc cgcgacgacg gccgtgagat acgcgacgaa ccaccagacg
     3241 gtctcccgca cgccgccgaa cagcagcgcc atcagcaccg caatgaacga ccacaggatc
     3301 accagactgg atccgacgaa gcctccgagg gcgagctgca gggcgaacgg cagtatcagg
     3361 atcaggaact gctgcgtttg ccggaacagc tcgtagcgcc gcaggcggga caacacgagg
     3421 aggtccagga gtgtcagaac cgagtatgca gccggtatcg cggcgacggt gcgctcaccg
     3481 aaggcgaaat agagcgcgcc ccagaccaat ccggccggga tcacgaggat ggaggcgatc
     3541 gtgaactgga ccttgcggcg cctggtctcc tcgtcgtcgc ccggagagta cgagatcctc
     3601 tccaaccacc cgagggacgt ggtcaccggc tgcaccgtca agcgctacgc ctccgctcgt
     3661 tcgaccaaca ccgttgaggc agcagagctc cctggcgagc cagccgcacc gcgtccaaga
     3721 taacggcaca cgcctgaaac gccctgccgc ggtgtgaaga cgggcggccg ggacgcagca
     3781 ggggtggggg actcagctcg gccgacggcg ggcgaccacg acgccggcca cgcacagggc
     3841 gccgccgagg atggcgaccg ccactggcac ctcaccgagg atcaaccagc tcatcccgat
     3901 cgtgatcggc ggcacgaggt aggccgtggc ggcgaggcgg ctcgccgtgc cgcgcgccag
     3961 ggcatacgcc cacgtcgtga aggcgacgga ggtcggaaac actccgaggt agaccatcca
     4021 ggccaggtcc accgacgacg cggtatggac ggcgcgcccc aacgacggtg cgtagggcag
     4081 gcagcacagc gcgccgatcg cgcagcaggt ccatgtgacc tgcagcgcgg acaaccgggt
     4141 gagcaccggt ttctgcgcca cgacaccgcc ggcggacgcg gtggtggcga ggacgctgag
     4201 taggacgccc cacggagtag cggtgcccga catcgtcgcc agaccgatga cgatcacgcc
     4261 gccgaaggcc actgcgcccc cggccagcaa ccgcgaggtc aggcgctccc gcaggaacag
     4321 cgccgccagc gccatgatca tgagcggcgc gatgttgacg accatggcgg cggttcctgc
     4381 atcgaccacc cgctccgacg tattgagcgc gacgttgtag aaaccgaacc acaacaggcc
     4441 ggcgaccagg agcagcccta gctcgcgccg cgtggggcgg agcccaccgc gtcgccacag
     4501 cagcaggccc agcagcacgc tcccgatggt gagtcgtccc agcgccaggg ccccgggcgg
     4561 gatcgtgcgc gtcgcggcgc gaatgccgac gaaggccgaa gaccacagcg tgaccgagac
     4621 gagcgcggcg gtgagcggcc gccagtcgtg cgactccaac gcgttctggg tcatggtcgt
     4681 ggcacgtaaa ggagtgctac gcatgcagca ccttgaacgc gtgcgtccac tcgttgtgca
     4741 ggaagcccgg gatgcaccac aggatgcaca gcggctcgat cgcgcgcgcc gcctccacgg
     4801 cccccaggtc cagggtctcc cagccgaact gatcgagtac cccgctcacg atccgtttcg
     4861 cggcggtgtc gttgccgcag atgaacatcg tggggcggcc ggccgcgtac tgcggattca
     4921 ccatcttcgc gttccccacg gagttgaacg ccttgacgaa gcgtacgtcg ggggacgcct
     4981 gctggagccg ttccatcagg gaatcatcga gcgaggtgaa gaacttgagc acaccattca
     5041 ccggcggggc ggcggcgatg gggttggtcg tatccaccac gaccttgccg gcgagcgcgg
     5101 ttgggccggc gaggcgcagc gcgtcgccgg cgacgtcgcc tttcacggcg aggacgacga
     5161 tctcgccgaa cgccgccgcg tccgcgaacg agccgatccg ggcgccggca tgctgggcac
     5221 gccactgggc gagcttcgcg gcgtcgcgcg tcccgagcat gacggcgtag ccgtgcttca
     5281 ggaaaccggc gccgagcgac tgcgcgacgg ggcccgatcc gatgatgccg acattcatgg
     5341 tgttatccct tcctcctgaa ccgctcgaag cggtacgcta attccggacc cccgtccccc
     5401 tcgtacgcga gctgtgcaat gagcagcgtg aacggctcga tgtaccgggc gatccgtaag
     5461 ggcccggcgt ccaccggatc gaaccccacg tcgcgaatca gctgagcggc aatgcgtttg
     5521 ctgcgcgcat catccccgca atacaccagg ctgggtttgg tggctttgcg ccgagcctcg
     5581 aacacaccaa acagcacttc gctcggcacc gtgccgaagg cggagacgac ccgagctccg
     5641 gcaatccgtt ttgcgagcgc ttccgcgccc gaggaggtgt gggcgatcac gaggtcggtg
     5701 tcgtccgcag tcatcgggag cgagcaggtg acgatgacct tgcccgacag atcgcccgct
     5761 tgccgcacca catcgtcgac tcgagaccag tgcaccgcca gcaggagcgc gtccgcctca
     5821 cgcgcggctt cgcccggagt gccagcgcgc gccttacccc tggcctcgcg cgcgagccgc
     5881 tgaagcttct gcgcgctgcg ggcgtagctg aataccacct cgtggccggc gcgcgtccag
     5941 atcgttccga gctttgcgcc catcaagccc gaccccagaa tgccgattcg catggttcct
     6001 ccactgcgtc agcggtcgat gaaccgctgc gagctttcgg cgtaccgctc gccgtgcacg
     6061 gtgatcgttg cggcggcact ctcgatctcc tggagatcgt ccggcgtgag gtcgcgtgcg
     6121 gttgcgccga ggttctcgtc cagccggtgc cgtttggtgg tgcccggaat cggaacgatc
     6181 cagggcttcc gggcaagcag ccaggcgagc gcgatttggg ccggcgtcgc gcgtttcctc
     6241 gccgcgactc tgacgaccag atccaccatg gactgattgg ccatccggtt ctccgccgcg
     6301 aagcgcggga cggtgttccg gaaatcggaa cggtcgaacg tcgtggtcgc gtcgatgctg
     6361 ccggtgagaa agcccttgcc cagtgggctg aaaggaacga aaccgatctg gagggcctcg
     6421 accgtcggca ggatctccgc ttccggctct ctccaccaga gcgagtattc gctctggagt
     6481 gcggcgatcg gttgaaccgc atgggcgcgt cggattgtct gcgcgccggc ctccgagagg
     6541 ccaaagtgct tcgccttgcc ctcccgaatc aggtcctgca ccgtcccggc gacgtcctcg
     6601 atcggcacgt tcgggtccac gcggtgctgg tagagcaggt cgatgcattc gcacttgagt
     6661 cgtcgcagca tgccctcgac cgcggtcctg atgtgctcgg gacggctgtt cacgccgcca
     6721 tggtgctgcc ccgtcaccgg atcgatgtcc cagccgaact tggtggcgat gaccacctgt
     6781 ccgcggaacg gcgccagggc atcgcccacc agctcctcgt tcgtgaacgg accgtaggcc
     6841 tcggcggtgt cgaagaacgt gacgccgccc tccacggccg cacgaatgag ggcgatcatc
     6901 tcccggcggt ctgccgccgg cccgtagccg aagctcatgc ccatgcagcc gagcccgatc
     6961 gccgagacct ccaggttact cgttccaagt gtgcgcttct gcatgttcct cctcgcgtct
     7021 tccggtcagc ggtgtacggg cccctcgccc agacagccat gggccgaagc cgtggagtac
     7081 cgcatcgagc ggtgcgccgc taatttaggg cctgccgcca ccgtagctca gtggtagagc
     7141 tctcgattcg taatcgagcg gtcgtcggtt caagtccgac cggtggctct tgtctcaccg
     7201 cggtccttcg tcacactcct tccgatttgg gccgaagagc agggcgggta gcgtggactg
     7261 cttcacgagc cgcaaccggc tgccgtgcag gatgagggtg tcgcctcgga tatgggcggc
     7321 gtcggtttcc gagcgcgtcg gcgactgcag agagagccgc aatacattgc cgtctcgaac
     7381 tccggtgaca ccgcggatgc cggcggctcc gccgtcgtgg tcgacgagaa ctctcaccaa
     7441 tccaccgggc gcatccacca ggaccagctc gacactactc cgcctgcggg gactcgcctc
     7501 ggtataggcc ccgctaatgg actcgctggc ggacaccagg gtcgctgcaa ttctccgcag
     7561 atcgagctgc ggtgtatcga tcaacgtccc cggttgggtc cgcgcttgcc tcatgctccc
     7621 tgtcagcccg gaagcggacc agtgtcccac gaatcggtag gccgctccgt cccccgtact
     7681 cgatgtgcga aaggagacct ctccgccgcc gaacaccctc aggtcacaaa cggcgagcgt
     7741 gtggggtgtc aggtaggccg cgctcgagtc cgatcccggc ttcagataga tcagccatgg
     7801 accgccgtcg gccggtccgg cccagaagag gccctgcatc gcctgttggg acggtgacgt
     7861 acacgccagg gcgagaatgg accacggcat acacaccagt gagtacgagg tggacctgtt
     7921 cccagccatg cgccgattca cctcgtcagg cggagtccga gccggcatcc gcggctacgg
     7981 cgggtgccgg cggtgtttac gacgcggtca cgtgccccac acggcagtgt ggaactgcgc
     8041 cgagcggggc gccagcttcc cccgtccgct cgagtagtcc ttccagctga cgatgccgca
     8101 gtcattcggg atgcgccgca tcgtgatgca cccttgcacg gggcacacct ccgagcagag
     8161 gttgcacccc acgcacgcct cctcgatgat ctcgaccacc gggccacccg tccctcgtcc
     8221 gccgtccccc gtccctcgcc tctgatctcg cgtcccgagg gttcggatcg cctgatgcgc
     8281 cccgtcctcg caggcgacga agcacaggcg gcagccgatg cacgtcgcct gatcgatctc
     8341 ggccacgagg tgatagttga ggtcgaggtc ctcccacttc accacgttgg gcagcgcccg
     8401 cccacgcacg tcatcgagcg tacgatggcc ggccccgtcc atccacatgg tgagcccgtc
     8461 caccaggtcc tcgatgatgc ggaacccatg gtgcatgacc gccgtgcaga cctgcagcgt
     8521 ggtggcgccg agggccacga actccgcggc atcgcgccag gtggcgatgc cgccgatccc
     8581 ggaaatgggg acgcgcacgg cggggtcacc ggcgacggcg gaaacgagat gcagcgcgac
     8641 cggcttcacc gccggcccgc agtagccgcc gtggctcgac ctgccgccga cgtccggtaa
     8701 gggagcgaag cggtccaggt cgacgccgac gatcgagttg atcgtattga tcagcgacag
     8761 cgcgtccgct ccacccttga ccgccgcccg ggcgatgtag gtgatgtcgg tgacgttggg
     8821 ggtgagcttg acgatcaccg ggatcgcggc cacttccttc acccactcca cgatctggca
     8881 ggtgtagtcg ggcacctgac ccaccgcact gcccatgccg cgctcgctca tgccgtgcgg
     8941 gcagccgaag ttcagctcga tgccgtcggc gccggtgtcc tgggtacgct ggaccatgtc
     9001 gtgccagact ccgcgcttcg actccaccat cagcgacacg atgacggcat ggcgggggta
     9061 gttccgcttg accgccgcga tctccttcag attgtcggcg acgggccggt cggtgatcag
     9121 ctcgatgttg ttgaacccca tcatccggga gctgcccaaa tccaccgagc cgtagcgtga
     9181 gaacacgttg atgatcggct cccccaccgt cttccagacg gcaccacccc agccgaggtc
     9241 gaaggcccgc gccacctggc cgtaggtgtt ggtgggcggt ccggaggcga gccagaaggg
     9301 gttggggctc tcgatcccgg cgaacgtcgt atgcagatct gccatgcctg cctccctatt
     9361 ccgtcttctt ccgccctctt ccgccttctt ccgcgagcat ctcgtcgatc ttcgccgcgg
     9421 cccgcacgcc ttccgccgcc gcgttgacga tctcggcgcc gccgttggcg tagtcgcctc
     9481 cgacgaacac cttgggatgc gacgggagca gaaactcgtc gcgtgcggcc tggccgagcg
     9541 cggtgatgat catgtcgcac ggcaacacct cgatctctcc cgcgtgctcc acttcgagcc
     9601 cctcgacatg gtgcacaccg aggatgcgtc gcggcgtaca gtggaagcgg aacacgcacc
     9661 cgtcccgttg cgccagctcg tactcgtagt cgaagcaggg catgtcggtt tcgccgcgac
     9721 ggtacacgat cgttgcgtcg gacgccccca ggcgcttcgc ctgggtcacc gcatcgatcg
     9781 ccgtgttgcc cgcgccgatc accaccacgc gccggcccac gcgcgtctcc cgatagggat
     9841 gagtcttcag gtgggcgatg aacgtcaggg catcgaccac gcccgccagc gattcgcccg
     9901 ggatcccgag ccgattcgtg agtcccaggc ctacacccac gaacaccgcg tcgtagtcgg
     9961 cgagcagcgc gtcgaacgag acgtcgcggc cgatcatgac gccggtgcgg agctcgatgc
    10021 ccaggtacag gatgtcggcc acctcggcca gggcgacggc cggcgtcatc ttgtattcgg
    10081 cgatcccgaa ggtgttgagc ccgccggggc ggtcctgcgc ctcgaacacc gtcacggcgt
    10141 gaccccggcg caccaggtcg cgcgcgcacg agagccccgc cgggccggcc ccaacgatcg
    10201 ccacccgctt gccgctgggc ggtcccggct cgaacagccg gaggtggtgc gccagcacgt
    10261 gatcggtggc gtgacgctgc agcagcgcga tcttgatcgg ccgctcatcc cgctgaatga
    10321 gcacgcaggc gccttcgcac aagacctcga cggggcaggc gcgggcacaa gagtgaccga
    10381 acgga
//
LOCUS       DBNGZW010000121         3662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1711250, whole genome shotgun sequence.
ACCESSION   DBNGZW010000121 DBNGZW010000000
VERSION     DBNGZW010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3662
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1711250"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..1086
                     /locus_tag="AC16JC_02995"
                     /pseudo
     CDS             115..1086
                     /locus_tag="AC16JC_02995"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            1083..2018
                     /locus_tag="AC16JC_03000"
     CDS             1083..2018
                     /locus_tag="AC16JC_03000"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5163693.1"
                     /translation="MTALSWLVLAAIVAWAIALVQWCRRRARRARRDDARRFFRHPTV
                     AASLAVLAVLVTVALCAPLVAPYRPSQQLDIIALQNRPPDWLHPLGTDLYGRDVWSRL
                     VYGARVSLGVGTLGMLVAVTLGGLVGATAGYFRGWVDGVLMRFVDVGLSVPRIFLVLG
                     AIAVWERLGIASLILLLGLTGWFATARIVRAEVLTLRERPFTDAARSLGAGSWRIIVR
                     HIIPNAAAPLIVSAALGIGNMMLLEAGLSFLGVGIQPPSPSWGNMIADGRDLLTSAPW
                     ISLFPGLAIALAVMALHAVADALRDALDPRTSRLS"
     gene            2026..3039
                     /locus_tag="AC16JC_03005"
     CDS             2026..3039
                     /locus_tag="AC16JC_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019121876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5163694.1"
                     /translation="MTEPLLRVRDLRTYFVTDRGGGTARAVDGVSLDLYPGETLGVVG
                     ESGCGKTVMSLSILRLVPEPPGHIRPGSLIEFEGHNLLTLPPRDLRAVRGNRIAMIFQ
                     EPMTSLNPVLTIGDQVAEAAIVHQHLSRGVARRRAIELLRQVGIPEPEERVDHYPHQL
                     SGGMRQRVMIAMALICRPQILIADEPTTALDVTIQAQILELLARLRQELGMAVLLITH
                     DLGVVAGTADRVVVMYAGQVVETAPTPELFAHPRHPYTEGLMESIPRLDRPRARLHSI
                     PGSVPAATAWPQGCRFHPRCPYAWDKCRTEEPPLLETGTDGHTARCWLVAEPARRTRG
                     PGG"
     gene            3036..>3662
                     /locus_tag="AC16JC_03010"
     CDS             3036..>3662
                     /locus_tag="AC16JC_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5163695.1"
                     /translation="MTAPTATPLVQVRDLVKHYPGERAWLGLGRPRSPVRAVDGVSFS
                     IPAGRTLGLVGESGSGKTTVARTLLRLTEPTSGTFLLDAIDVFALAPGPLRRLRRRLQ
                     IVFQDPFGSLNPRMTVRQTLREPLAIHDLAPRAERDGRVDALLGEVGLDPELADRYPH
                     ELSGGQRQRVGIARALSVEPAFLVCDEPVSALDVSVQAQVLNLLADLQQ"
BASE COUNT          420 a         1371 c         1250 g          621 t
ORIGIN      
        1 gtggattcgc gcgtgaccga cgtccagatc cgtccggact cctggtgggc cctggtccgg
       61 acctggcgca ttcctcccga caagctgaac gagcgggatc gcgtaggagg ctgagtgacg
      121 gcgtggctcc tgcggcggct cgctgccgga atcgccatcg tcttcgcggt cgtcacgctc
      181 acgttcgtcg cggtgcatct ggcgcccggc gacccgttcc tgcccgccga tggccgcccc
      241 atggacccgg cggcggtcgc gcggctgcgc acgacgtacg gcctcgaccg gccgttgctc
      301 gagcaatacg gccgctacct ggtggcactc ggccggggtg agctgggaca gtccttcagc
      361 agacggcgtc ccgtggcggc cgcgatcgga agcgccctcc ccaacaccgt cgtcctcgcc
      421 ggcgcggccc tgctcatcga tttcgcctgc ggcatcgcca tcggcgtctt tcaggcggca
      481 cgcgtgcggc gccgctcgga cgtcgcgctg acgaacctca cgctcttctt ctattcgctc
      541 ccgaccttct ggctcggcct ggtgctcctt ctggtgttcg gccagtggcc gcactggttg
      601 ccggtcggcg gcgcgatcga cccggtgctg cacgacgcgc tgcccctcgg cggccggctg
      661 ctggaccggc tgcggcatct cgcgttgccg gccctcacgc tcggtctcgt cggcgccggc
      721 ggcaccgcac gcttccagcg tgccgccctg ctcgaggcgc tggggcagga ctacgtccgc
      781 accgcacgcg ccaagggact ggccgagcgc accgtgctgg tccgtcacgc actgcgggac
      841 gcgctgctcc ccgtgatctc cctgctgggc ctggccctgc cgttcctgct caccggcgcg
      901 gtactggtct aaaccgtgtt cgcctggccc gggctcggca agctcgcggc cgacgcgatc
      961 ctggcccgcg actaccctct ggtgctcggg accgtgctgc tcgcgagcct cgcggtcgtc
     1021 gtgggcagtc tcgtcgccga tctgttgact gtcgccgccg accctcgcat ccggctgtcc
     1081 gggtgacggc cctcagctgg ctcgtcctgg ctgcgatcgt cgcgtgggcc atcgcgctgg
     1141 tgcagtggtg ccgtcgccgc gcgcgccggg cccgtcggga cgacgcacgc cgcttcttcc
     1201 gccatccgac cgtcgccgcg agtctcgcgg tgcttgccgt cctcgtgacc gtcgcgctgt
     1261 gcgctcccct cgtcgccccc taccggccgt cgcagcaact cgacatcatc gccctgcaga
     1321 accggccgcc cgactggctg catccgctcg gcaccgatct ctacggccgc gacgtgtgga
     1381 gtcgtctggt gtacggcgcc cgcgtgtcgt tgggcgtcgg gacgctgggc atgctggtcg
     1441 cggtgacgct gggcggcctg gtcggcgcga ccgccggcta cttccggggc tgggtggacg
     1501 gcgtgctgat gcgcttcgta gacgtggggc tctccgtgcc gcgcatcttt ctggtgctcg
     1561 gcgccatcgc agtctgggag cggctcggca tcgcctccct gatcctgctg ctcggtctca
     1621 ccggctggtt cgccaccgcc cgcatcgtgc gcgccgaggt cctgacgctg cgggagcggc
     1681 cattcaccga cgccgcacga tccctcggcg ccggatcatg gcgcatcatc gtccgtcaca
     1741 tcatccccaa cgccgccgct ccgctcatcg tgtccgccgc gctcggcatc ggcaacatga
     1801 tgctgttgga ggcgggactc tcgttccttg gcgtgggcat ccagcctcca agcccgagct
     1861 ggggcaacat gatcgcggac gggcgggatc tgctcacctc cgctccctgg atctcgctct
     1921 ttcccggctt ggcgatcgcc ctggccgtga tggcgctgca cgccgtagca gacgccctgc
     1981 gcgacgcgct cgaccctcgg acgtcgcggt tatcttaggg acgccatgac ggagcccctc
     2041 ctccgcgttc gcgacctgcg tacctacttc gtgaccgacc gcggcggcgg aaccgcgcgg
     2101 gcggtggacg gcgtctccct ggacctctat ccgggggaga cactcggcgt cgtcggcgaa
     2161 tcggggtgcg gcaagaccgt catgtcgctc tccatcctgc gcctcgtccc cgagccgccc
     2221 ggccacatcc gtcccggcag cctgatcgag ttcgaaggtc ataacctcct gacgctcccc
     2281 ccacgcgacc tgcgggccgt ccgcggcaac cgcatcgcca tgattttcca ggagccgatg
     2341 acctcgttga acccggtcct gacgatcggg gaccaggtcg ccgaagcggc gatcgtgcac
     2401 cagcacctgt cgcgcggcgt ggcccggcgc cgcgccatcg agctgttgcg gcaggtgggc
     2461 atccccgagc ccgaggagcg ggtcgatcac tacccgcacc agctctcggg tgggatgcgg
     2521 cagcgggtga tgatcgccat ggcgctgatc tgccgccccc agatcctcat cgccgacgag
     2581 ccgaccacgg cgctcgacgt caccatccag gcccagattc tggagctgct cgcgcgcctg
     2641 cggcaggagc tcggcatggc ggtgctgctc atcacccacg acctgggagt ggtcgcgggc
     2701 accgccgacc gcgtcgtcgt gatgtacgcc ggccaggtcg tggagaccgc gcccacccct
     2761 gagctgttcg cgcacccgcg gcacccctac accgagggcc tgatggagtc catcccgcgg
     2821 ctcgaccggc cgcgggcgcg gttgcacagc atcccgggct cggtgcccgc cgccacggcc
     2881 tggccgcagg gctgccggtt tcatccgcgc tgcccctacg cgtgggacaa atgccgcacc
     2941 gaggagccgc cgttgctcga gaccggaacc gacggccaca ccgcccgctg ctggctcgtg
     3001 gccgagccgg cgcgccgcac caggggtccc ggcggatgac cgcgccgacg gcgactccgc
     3061 tcgttcaggt gcgcgatctg gtgaagcact atccggggga gcgcgcctgg ctcggtctgg
     3121 gtcgtccgcg gtcgccggta cgggccgtgg acggcgtgag cttctcgatc cccgccggca
     3181 ggacgctggg cctcgtgggg gagtcgggct cggggaagac cacggtggcc cggacgctgc
     3241 tgcgcctcac ggagcccacc agcggcacgt ttctgctcga cgcaatcgac gtcttcgcgc
     3301 tcgcccccgg cccgctgcgg cgcctgcgac gccgtctcca gatcgtcttc caggacccgt
     3361 tcggctcgtt gaacccgcgc atgacggtgc ggcagacgct gcgcgagccg ctggccattc
     3421 acgatctcgc gcccagggcc gagcgtgacg ggcgggtgga tgcactgctc ggcgaggtgg
     3481 gtctcgatcc ggagctggcc gatcgctatc cgcatgagct gtccgggggc cagcgccagc
     3541 gggtgggcat cgcgcgggcc ctgtcggtcg agccggcgtt cctcgtgtgc gacgagccgg
     3601 tgtcggcgct cgacgtgtcg gtccaggcgc aggtgctcaa cctgctcgcc gatctgcagc
     3661 ag
//
LOCUS       DBNGZW010000122         4288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1170991, whole genome shotgun sequence.
ACCESSION   DBNGZW010000122 DBNGZW010000000
VERSION     DBNGZW010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1170991"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3803)
                     /gene="gltB"
                     /locus_tag="AC16JC_03015"
     CDS             complement(<1..3803)
                     /gene="gltB"
                     /locus_tag="AC16JC_03015"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF008730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HIR5163696.1"
                     /translation="MAQASGARSTTILRHALQALARVAHRGAVATDHSGDGAGVLTQI
                     PVALFQRDAARLQLRLAPGQPFAVAVVFLPGEPEARARVTDIIEAVLAGEGLPLLGWR
                     DVPVEPRALGAHARATCPVIRHALVGPPAGASGDELAWERALYVARRTIERRVAESGP
                     GLDAFFVCSFSCRTIVYKALLTGTDLARFYPDLRAPDFETAIALFHQRYSTNTTSSWP
                     LAQPFRLLAHNGEINTLWGNRNAMRAREPALASPVWGDAVERLKPVIWAEGSDSASLD
                     NALELLVRSGRDPVHALMMLVPEAHGAGARIDPALRGFYEFHECLMEPWDGPAALAFS
                     DGRRAGAALDRNGLRPCRYQITAGGLVVAGSEAGLVDLDPGQVVETGRVGPGELLVVD
                     TGRQTILRNAEAKREVARREPYARWVGRSVLRLHPATSQTDATSPDAAELATRQLAFG
                     WSFEDLRYVLEPMANGQDPVWSMGDDTPIPPLALRPPVVYAFFRQRFAQVTNPPIDSL
                     RETLVMSLRMHLGRRTSLLSPQPAGLRLVRNEHPILLEEEMATLRQVAGVQVVTVDAT
                     WRAARGPGQLRVALDRLCRTVGRAVHEGARIVMLSDRNVDRERAPIPMLLAVGAVHHH
                     LLQKGLRGRVGLVAEAGDAWDVHHVATLIGYGAEAVHPWLALQAVRAVAGDEGRQRFR
                     GTAEKGLLKVLSKMGISTLASYHGAQIFEALGLGAEVIERCFVGSVSLLGGLGFAEIA
                     EDVLARHHAAYGDAGSQELPDHGRVRYRRDGEDHGWSPPLVRALQGATFDAFVSQVAA
                     RPPAGPRDLLTLRRGRPVPLHEVEPAQAICRRFVSTAMSLGALSPEAHRVLAIGMNRM
                     GARSNSGEGGEDPDTYAPVPNGDRADNRIKQVASGRFGVTTQYLVRADELEIKIAQGS
                     KPGEGGQLPGHKVTALIARLRHSVPGVPLISPPPHHDIYSIEDLAQLIHDLKQVNPRA
                     RVGVKLVAEAGVGRVATGVAKAYADYVLVSGHNGGTGASPLSSIKHAGSPWELGLAET
                     QALLVENGLRHRVELRVDGGLRTGRDVVVAALLGAEAYGFGTAALVAMGCAMARQCHL
                     NTCPTGIATQDPKLRARFRGTPEQVVAYFTWVAEEVRRLLASMGARTLEEIVGRVELL
                     ERMDRPDLPRAQMLDLSGLLAPAGGRTSTRRRRAARNDRPGVVSLDETILPALERELD
                     AGRPFAGEYRIGNHHLTVGARIAGRIVERRGGGALAPDSIRLRFHGTAGQSFGAFAVT
                     GMCLEL"
     gene            4003..>4288
                     /locus_tag="AC16JC_03020"
     CDS             4003..>4288
                     /locus_tag="AC16JC_03020"
                     /inference="COORDINATES: protein motif:HMM:NF012354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HIR5163697.1"
                     /translation="MHLDTLRLYADLVRLRSYSRAAEQHQVSQSAASQAIRKLEADLG
                     ATLVDRSQRPLVLTPQGQAFHEACLGVLAAWEQARAAVSAAGPRIDGTVRL"
BASE COUNT          627 a         1558 c         1536 g          567 t
ORIGIN      
        1 agctcgaggc acatgccggt cacggcgaac gcgccgaaac tctggccggc ggtgccgtgg
       61 aaccgcagcc gaatcgagtc gggcgccagc gcgccgccac cgcgtcgctc gacgatccgg
      121 cccgcgatcc gcgcacccac cgtgagatga tgattcccga tgcggtactc gccggcaaag
      181 ggccggccag catcgagctc gcgctccagg gcggggagga tcgtttcgtc cagcgatacg
      241 acccccggcc ggtcgttccg cgcggcgcgg cgccggcgcg ttgacgtgcg accgccggca
      301 ggggcgagga gaccggagag atcgagcatc tgggcgcgcg gcagatcggg ccggtccatc
      361 cgctccagca gctccacccg gccgacgatc tcctccagcg tacgtgcgcc catggaggcg
      421 agcaggcgtc gcacctcctc cgccacccac gtgaagtagg cgaccacctg ctccggcgtg
      481 ccgcggaacc tggcccggag cttcgggtcc tgcgtcgcga tgcccgtcgg gcaggtgttg
      541 agatggcact gccgcgccat cgcgcacccc atggcgacca gcgcggcggt gccgaagccg
      601 tacgcctccg cgcccagcag cgcggccacg accacgtcgc gcccggtccg caggccgccg
      661 tccacccgca gctcgacgcg gtggcgcagc ccgttctcga ccagcagcgc ctgggtctcc
      721 gccagcccga gctcccacgg ggagccggcg tgcttgatgg acgagagcgg cgacgcgccg
      781 gtgccgccgt tgtggcccga gacgagcacg tagtcggcgt aggccttggc gacgcccgtc
      841 gccaccctgc cgacccccgc ctccgcaacg agcttcacgc ccaccctggc gcgcggattg
      901 acctgcttca ggtcatggat cagctgggcg aggtcctcga tcgagtagat gtcgtggtgc
      961 gggggcggcg agatcagcgg cacgccgggg accgagtggc gcagccgggc gatgagcgcc
     1021 gtgaccttgt gccccggcag ctggccgccc tcccccggct tcgagccctg cgcaatcttg
     1081 atctccagct cgtccgcccg caccagatac tgggtggtga cgccgaagcg gcccgaggcc
     1141 acctgcttga tccggttgtc ggcccggtcg ccgttgggaa cgggcgcgta ggtgtccgga
     1201 tcctcgccgc cctctccgga gttggaccgt gcgcccatcc ggttcatccc gatcgccagc
     1261 acgcgatggg cctcgggaga gagcgctccg agcgacatgg ccgtggacac gaagcggcgg
     1321 caaattgcct gggccggctc cacctcgtgc agcggcaccg gccggccacg ccggagggtg
     1381 agcaggtccc gcgggcccgc agggggccga gccgcgacct gggagacgaa ggcgtcgaag
     1441 gtcgccccct gtagcgcgcg caccagcggt ggggaccagc cgtggtcctc gccgtcccgg
     1501 cggtagcgga ctcgcccgtg gtcgggcagc tcctgcgatc ccgcatcccc gtaggccgcg
     1561 tggtgacgcg ccagcacgtc ttcggcgatc tcggcgaagc cgagaccccc gaggagggag
     1621 accgacccga cgaagcagcg ctcgatcacc tcggcgccga gacccagcgc ctcgaagatc
     1681 tgcgcgccat ggtaggatgc cagcgtcgag atccccatct tggacagcac cttgaggagt
     1741 cccttttccg cggtgccgcg gaatcgctgc cggccttcgt cccctgccac cgcccgcacc
     1801 gcctggagcg ccagccaggg gtgtaccgcc tccgcgccgt acccgatcag cgtcgccaca
     1861 tggtgcacgt cccaagcgtc ccccgcctcg gcgaccaggc cgacacgtcc gcggagcccc
     1921 ttctggagca ggtggtggtg caccgcgccc acggcgagca gcatgggaat gggcgcccgt
     1981 tcccggtcca cgttccggtc gctgagcatg acgatgcgcg cgccctcgtg cacggcgcgg
     2041 cccacggtgc ggcacaggcg atcgagcgct acgcgcagct ggcccgggcc acgggcggcg
     2101 cgccaggtgg cgtccacggt caccacctgg acgccggcga cctgacggag cgtggccatc
     2161 tcctcttcca gcaggatcgg gtgctcgttt cgcaccaggc gcaggcctgc cggctgcggg
     2221 gacagcagcg aggtacgccg tccgaggtgc atccgcagcg acatcacgag ggtctcgcgc
     2281 agcgaatcga tcggcgggtt ggtcacctgc gcgaagcgct gccggaagaa ggcgtagacg
     2341 accggggggc gcagtgccag cggggggatc ggcgtgtcgt cgcccatgct ccacaccgga
     2401 tcctgcccgt tcgccatcgg ctccaggacg tagcgcagat cctcgaagct ccagccgaag
     2461 gcgagctggc gggtggccag ttcggcggcg tcgggcgacg tggcgtcggt ctgagacgtc
     2521 gccggatgca gtcgcagcac gctgcgcccg acccagcgcg cgtagggctc gcgccgcgcc
     2581 acctcgcgct tcgcctcggc gttccggagg atcgtctggc gcccggtatc caccaccagc
     2641 aactcgcccg gccccacgcg acccgtctcc accacctggc caggatccag gtccaccagg
     2701 cccgcctcgg atccggcgac gaccaggccg ccggcggtga tctggtagcg gcagggtcgc
     2761 aggccgttgc ggtcgagagc ggcaccggcc cggcggccgt cgctgaacgc cagcgccgcg
     2821 ggcccgtccc agggctccat cagacactcg tggaattcat agaagccacg cagcgccgga
     2881 tcgatcctgg ctcccgcgcc gtgcgcctcg ggcacgagca tcatgagtgc gtggaccggg
     2941 tcgcgtcccg agcgcaccag cagctcgagc gcgttgtcga gactcgccga gtcgctcccc
     3001 tccgcccaga tcacgggctt cagccgctcg accgcatcgc cccagaccgg cgacgcgagt
     3061 gccggctcgc gcgcgcgcat cgcgttgcgg ttgccccaca gggtgttgat ctcgccgttg
     3121 tgcgccagga ggcggaacgg ctgggccagg ggccagctcg aggtggtgtt ggtcgagtac
     3181 cgctggtgaa acagggcgat cgcagtctcg aagtcgggcg cccggaggtc gggatagaaa
     3241 cgggccaggt cggtgcccgt caggagcgcc ttgtagacga tcgtgcggca ggagaacgag
     3301 cacacgaaga aggcgtccag gccgggacca ctctcggcca cgcgccgctc gatcgtacgg
     3361 cgtgcaacgt agagggcgcg ctcccacgca agctcgtcgc ccgaggcgcc ggccgggggc
     3421 cccaccagcg cgtgccggat gaccgggcag gtggcgcggg cgtgcgcgcc cagcgcgcgc
     3481 ggctccacgg gtacgtcgcg ccaccccagc aacgggaggc cctccccggc gaggaccgcc
     3541 tcgatgatgt cggtgacgcg agcccgggcc tcgggctctc cggggaggaa gacgacggcg
     3601 acggcaaacg gctggccggg cgcgagccgc aactgcagac gcgcggcgtc gcgctgaaac
     3661 aaagcgacgg ggatctgggt gaggacgccg gcgccgtcgc cggagtggtc ggtggcgact
     3721 gcgccgcgat gggccacccg ggcgagcgcc tgaagggcat ggcgcaagat cgtcgtggag
     3781 cgcgcccccg acgcctgcgc cacgaatccg acgccgcatg cgtcgcgctc gcgccaggga
     3841 tcggcacggc cgtcccccgg cctgccggac gccgcgtggc ggtggcgcgt cgtcaggacg
     3901 ctgtcttccc tggttccatg cccgcgacaa tagttgcacc attagcaaaa cacaagcggc
     3961 gacatcattg cattagaaaa tctaatttcg taccttgagg ccatgcacct ggatacgctg
     4021 cggctctacg ccgacctcgt gcgcctgcgg agctactcgc gggcggccga gcagcatcag
     4081 gtctcccagt cggcggcgag ccaggcgatc cgcaagctcg aagcggatct cggggcgacg
     4141 ctggtggatc gctcgcagcg gccgctggtc ctgaccccgc agggtcaggc gtttcacgag
     4201 gcctgcctcg gcgtgctggc ggcatgggag caggcgcgcg cggcggtttc ggcggccggg
     4261 ccgcgcatcg acggcaccgt tcggctgg
//
LOCUS       DBNGZW010000123         1271 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1579978, whole genome shotgun sequence.
ACCESSION   DBNGZW010000123 DBNGZW010000000
VERSION     DBNGZW010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1271)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1271)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1271
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1579978"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..144)
                     /locus_tag="AC16JC_03025"
     CDS             complement(<1..144)
                     /locus_tag="AC16JC_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012698622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HIR5163698.1"
                     /translation="MPSASIVVHVVPRAKTTEVAGRHGDAIRIRLAAPPVDGAANEEL
                     VRFL"
     gene            complement(173..754)
                     /locus_tag="AC16JC_03030"
     CDS             complement(173..754)
                     /locus_tag="AC16JC_03030"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR5163699.1"
                     /translation="MTFNVLLLIRYAVFAAFALAVLVALGSWLVRTRRLSPFGPVGRG
                     LRQLTDPLMRPVERRVVRAGGNPVQAGWWLVIGTAIIGILVIAIAQWLLTSWYSITGA
                     AHAGGRSMLVLAIGVVYDILFFAILVRVLASWLGRGRYTRWLRPVYWLTDWLVEPIRR
                     ILPTAGFIDFSPLVALLVLWLLRFFVVGVLLGG"
     gene            861..>1271
                     /locus_tag="AC16JC_03035"
     CDS             861..>1271
                     /locus_tag="AC16JC_03035"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5163700.1"
                     /translation="MRIRPFIVTVAAACAASCGTAREPVYIGVAGPFAQPRGTSMQLA
                     ARLAAKQLNDRGGIGGRQVRLVFADDSSQDGAALRAAQSFVDSDRVVAVVGHLSSGPT
                     LTALGVYAGAPNPIPLISPSASSPDLTGASKWMFR"
BASE COUNT          217 a          444 c          439 g          171 t
ORIGIN      
        1 gaggaagcgc accagctcct cgttggccgc tccgtccacc ggcggtgccg ccaggcggat
       61 gcggatcgcg tcgccgtggc gccctgcgac ctccgtggtt ttcgcccggg gtacgacgtg
      121 cacgacgatg ctggcactcg gcatcagacc cgacgaccga tgaccgaaga catcaaccgc
      181 cgagcaagac accaaccacg aagaaccgca gcagccacag caccagcagc gccaccagcg
      241 ggctgaagtc gatgaagccg gcggtgggga gtatccggcg gatcggctcg acgagccaat
      301 cggtgagcca gtagacggga cgcagccacc gcgtgtatcg gccgcgaccg agccaggagg
      361 cgaggacccg gacgaggatc gcgaagaaca ggatgtcgta gacgacgccg atcgccagga
      421 cgagcatgct gcgtcctccc gcgtgcgctg cgccggtgat ggaataccag gacgtgagca
      481 accactgggc gatggcgatg acgaggatgc cgatgattgc cgtcccgatc accagccacc
      541 agccggcctg caccgggttg ccgccggcgc gcaccacgcg gcgctcgacg ggccgcatca
      601 gcgggtcggt cagctggcgt aaccctcggc ccaccggccc gaagggagag agacgccggg
      661 tgcgcaccag ccagcttccc agcgccacga gcaccgccag ggcgaacgcc gcgaagacgg
      721 cgtagcggat cagcagcagg acgttgaagg tcatcgtact tggcagtggg tggagttata
      781 gatagccggg gatgggggca tgcgtcagcg tcctcgcgtc tggcgcatcc cggccgagtt
      841 gcccacatta gcggcccgtg atgcgcattc gccctttcat cgtgaccgtc gccgctgcct
      901 gcgcggcgtc ctgcggcacg gcccgcgagc cggtgtacat cggcgtggcc ggccccttcg
      961 cccagccgcg cgggacctcg atgcagctcg cggcccgcct cgcggcgaag cagctcaacg
     1021 accgcggcgg tatcggcggc cggcaggtgc gactcgtgtt cgccgacgac agcagtcagg
     1081 acggcgccgc gctgcgcgcc gcgcaatcgt tcgtggacag cgaccgcgtg gtcgcggtgg
     1141 tcggacatct gtcttcgggt cccacgctca ccgccctcgg cgtgtacgcc ggggcgccca
     1201 atccgattcc gctgatcagt ccgtccgcct ccagtcccga cctcaccgga gcctcgaagt
     1261 ggatgttccg g
//
LOCUS       DBNGZW010000124        10646 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1442840, whole genome shotgun sequence.
ACCESSION   DBNGZW010000124 DBNGZW010000000
VERSION     DBNGZW010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 10646)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10646)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10646
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1442840"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="AC16JC_03040"
     CDS             <1..641
                     /locus_tag="AC16JC_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR5163701.1"
                     /translation="DAIRPTARAAIASLHGLGARVLLLTVDSRPTAEAVARAIGIDEI
                     RAEVLPGDKATEVQRLERAGRRVAMVGDGVNDAPALAAASVGIAIGAGTDVAIETAGV
                     VLMKDDPSDVAAALTLARHVHRKIVQNLFWAAIYNVVAIPLAAGALYPSLGILLKPAW
                     AALAMSASTVTVTVNALLLPRLDSVTPSGFPSSGPRTGRERPSPVGLPRPRR"
     gene            complement(556..1701)
                     /locus_tag="AC16JC_03045"
     CDS             complement(556..1701)
                     /locus_tag="AC16JC_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009160504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HIR5163702.1"
                     /translation="MTFDMALVQRGARLRRTPYFAATQRAGARGYTVYNHMLFPICYG
                     DMVEEYWHLLRHVTLWDVAVERQVEITGPDAFHFTNLLTPRDLSRCAVGQGKYVVITA
                     ADGGIINDPVLMRLGENHFWLALADSDVLLWAKGVALNSGLKVEITEPDVSPLQVQGP
                     QSRQVIEALFGAGALQLPYYWFLQTDLDGIPVVVTRTGWSGEVGYEIYLRDGSRGEEL
                     WERVMAAGRPYDIRPTGPSDIRRIEAGILNWGADMTLANNPYEVGLQRLVDEGKTQDY
                     VGRDALRRIKAAGVQRRLVGLEIAGAPIAFNTTKWPVQRDGRPAGQVTSAIHSPRLQK
                     NIGYAMVPIDCASPGTGLTVSVADVGDRQATVVPFPFVDPRKEIPKG"
     gene            1821..4490
                     /locus_tag="AC16JC_03050"
     CDS             1821..4490
                     /locus_tag="AC16JC_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013220004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR5163703.1"
                     /translation="MTTRDRSAARGTAWHTRTVAEASRLLDVEPGAGLSSRRAAERLA
                     VHGPNALAAARRRGALRVLLAQFTDFMVLVLIAAAVVAGLLGEPQDIIAIVAIVVLNA
                     ALGFAQEYRAERAMAALTALAVPQAQVRRDGVVRSLPAPELVPGDVVLLEAGNLVPAD
                     LRLVEAVRLRVEEAVLTGESHPVDKTTAPLADPDLPLGDRSNMAYQGTLATSGRGVGI
                     VTATGMTTELGRVAGLLKDVEVARTPLQRRLAQLGARLTIAIVILCAVMFGFGLLRGE
                     DPGFMFLTALSLAIAGMPEALPAVVTISLALGARRMAKRNALVRRLPAVETLGSVTYI
                     CSDKTGTLTENRMRVEAYHTGDGRMPEPGGSAEREPWSLLLTALALCNDAIRGPDHDA
                     QGDPTEVALLLAAARAGREKAALEERYPRRGEIPFSPERARMTTLHHDGAEVLAFTKG
                     SPEQVLALCRDRLTAAGRVPLAREAVLEEAADMARDGLRVLAVAVRRLQGDPAALSSE
                     AIEAEETLLGLVGLLDPPRPEARDAVAQCQAAGIRVVMITGDHPATAQAIARSLGIAR
                     GADECMTGRELATVPAAELASRVAGVRVYARVAPEQKITIVQALQDRGEFVAMNGDGV
                     NDAPALRRADIGVAMGRSGTDAAREAAHMILLDDNFATIVGAVREGRRIYDNLRKFVK
                     YTLTTGVAEIWPLVLAPILGIPIPLLPIQILWVNLVTDGLPGLALAAERSEARVMARP
                     PRPPREGLFARGLWQHVVWVGFLMGSITLGTQLWGSQAGLHWRSMTFSVLALSQMGHV
                     LAIRSEDTSLFRLGLWSNPPLLGAVVVTLVLQLAILYVPGLNTLFGTQPLTGFELLVT
                     LALSSIVFVAVEIEKWVKRKGGLRVSQGDRRRG"
     gene            4459..5358
                     /locus_tag="AC16JC_03055"
     CDS             4459..5358
                     /locus_tag="AC16JC_03055"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5163704.1"
                     /translation="MFRKAIVVGVDGSSESLRAAAVARRIAEAVGGGVRLQVTHAVPE
                     ARLAKAVGPVPVYVSGLFARALAATRRDLAGRLRGVVPPAALETLDVRAGRAAQVLAD
                     SVARRRAGLVVLGGKRHGILARTVRGSTAHYLARVLDIPILVTGAGGRALGRVLVAVD
                     LSTAGGRTLAAGVGLARALGARVRVLHVVEPIRYAYVMLRAPDAKAVLERSRAALLRL
                     VARYGIAGTDTVVRRGAALGVIASEADEWRADLIVVGSQGKGFVDRLLIGSTTESLLA
                     RLPASLLIVPVAGRKSRRLRATR"
     gene            complement(5440..5580)
                     /locus_tag="AC16JC_03060"
     CDS             complement(5440..5580)
                     /locus_tag="AC16JC_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163705.1"
                     /translation="MGTLGRPLDRRSIGIGMIPRGKVGLIFASIGMTQTLAGAPIVDS
                     SI"
     gene            5669..6862
                     /locus_tag="AC16JC_03065"
     CDS             5669..6862
                     /locus_tag="AC16JC_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5163706.1"
                     /translation="MGLIASLCRASGAAIGCTLMLYAALDAQRPSEEPKRPSMEATRD
                     TNSGASYYLYGLSQLRSNPTRAAAAFYWATRLEPDMAEPWYGRWAALLLSEDPRVLGD
                     YLAGKPYIAKSRDIQQIDSLRYQAMIREPLLHWGLDQILLDEWLSRVSGGEVSLNMVV
                     RDYGPEMAGWWAYGQGQFLEALKQYAVAVKQNPKSYALRLARARSFLPLLQFDSAAAA
                     YAEVLTLQSGQEAERVVHVYDSKEIAYYTIGRIRETQRNVAAAREAYGQALVENLAFY
                     PAHAALARVALAVGDSAAAEHEYDQALQLHPDNPGAHYDYGVLLYALKRYEAAAEQLQ
                     LAVDEDPYYARPYFLLAYLRQYEGEDSVATALYRRFIQIAPASQAAQVKLARELLGEQ
                     AAPPR"
     gene            7102..8349
                     /locus_tag="AC16JC_03070"
     CDS             7102..8349
                     /locus_tag="AC16JC_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5163707.1"
                     /translation="MTNPEHDLAKLHIDRDAPPAVRRAFTRNLLLAAAAVALLGGGVL
                     FARRGGAVPVQVVIATAASGGGGAAGNASVTANGYVVARTRASVSSKVAGRLAYLGGS
                     EGSFVRKGDIIARLENADYRAAVAQAQAAVAAARADSIESTADRDQMEREARRLRTIH
                     AQNRELVAQQEVDQAESRAAQSAARAQATAARVEAALAGLRFAQANLDNTVIRAPFTG
                     TVLRKDAEVGEVVAPSVGGGLTRGAVVTMADLATLEVEVDVNEAYIARILGRQPARIT
                     LDAYPDTGFRGAVRQVVPTADRQRATVQVKVSITDRDPRILPEMGARVDFLEPDTAHA
                     PVAGATAPPRIRVPAAAVRDENGESVVWLVRQGRLERRAVQAGPVSGGFREIRSGLVG
                     GELLLAGGVETPRPGMRVKTATP"
     gene            8364..9053
                     /locus_tag="AC16JC_03075"
     CDS             8364..9053
                     /locus_tag="AC16JC_03075"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011301962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5163708.1"
                     /translation="MALAEIRDVHKSFQRDAQRIDVFTGLTLDIPEGSFTALMGPSGS
                     GKSTLLNLLAGLDRPTSGTVRVAGAQVSAMTPAQLATWRARHIGFVFQSFNLLPVLTA
                     YQNVELPLLLTRLSRKQRDERVRVALGVVGLDDRLDHYPRQLSGGQEQRVAIARAIVA
                     DPTLILLDEPTGQLDAKSSQEVLGLLGRLNDEFKKTIVIVTHDAHAAERAKTVLHLEK
                     GQLIEMAGASP"
     gene            9050..10192
                     /locus_tag="AC16JC_03080"
     CDS             9050..10192
                     /locus_tag="AC16JC_03080"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5163709.1"
                     /translation="MKYFPLVLANLGRHKLRTVLTIASVALALFLFASLRTVVTTLGA
                     AAQFGSARRLVVTNATGIVFPLPLAYANRLSTVSGVEGVSWANWFGGRYGDGKRFFAN
                     FAVDVESYLDLYPEIQLPPEQRAAFLQDRSSALVGRGLLNVFGWHLGQNVTLQGTIFP
                     GDWTFTIRGVYTPSDRAINDDMLLFHHDYFDERIGRAGIAGWYVVRINDPNRAAQIAK
                     TIDDQFRNSSSPTKTGTEQAFNASFATMWGNVRLLMGTIGIAVVFAILLVTANAMMMS
                     ARERTGEIAVLKTIGYPDRTLFGLVLLEAGVVTLTGALIGLGGAKLLYRATNFNAAGF
                     LPGFDVTRSTLVLGAAVALLLMLASGLVPAVRAARLPVIQALRHVE"
     gene            10189..>10646
                     /locus_tag="AC16JC_03085"
     CDS             10189..>10646
                     /locus_tag="AC16JC_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5163710.1"
                     /translation="MSIPLIYNVRSVLQRRVSTGLTALGIGLVVAVFIGTLALASGFR
                     AALVRTGSGGNVLVLRRGADSELASGLSRETASIIAASPHVATGPDGRPLVSPEVYVL
                     VPLPRPTDTTAVGNVVARGVSDQAWVVRTGVQIVAGHRPQSGRAEICVGRR"
BASE COUNT         1530 a         3641 c         3856 g         1619 t
ORIGIN      
        1 ccgacgcgat ccgcccgacg gcacgagcgg cgatcgcgtc gctgcatggc cttggcgctc
       61 gggtcttgct gctgacggtg gacagtcgcc ccacggcgga ggcggtcgcg cgcgccatcg
      121 ggatcgacga gatccgcgcc gaggtgctcc cgggcgacaa ggcaacggag gtgcagcgac
      181 tggagcgggc gggccggcgc gtggcgatgg tcggggacgg cgtgaacgac gcgcccgcgc
      241 tcgcggcggc ctcggtcggg atcgcgatcg gtgcggggac ggacgtcgcc atcgaaacgg
      301 cgggcgtcgt cctgatgaag gacgatccct cggacgtggc ggccgcgctc accctggccc
      361 gccacgtgca ccgcaagatc gtgcagaacc tcttctgggc ggcgatctac aacgtcgtgg
      421 ccattccgct ggcggcgggc gcgctgtacc cgtcgctcgg catcctgctc aagccggcat
      481 gggcggccct cgcgatgagc gcgagcacgg tgaccgtgac ggtcaacgcg ctgctgttgc
      541 cgcggctcga cagcgtcacc ccttcgggat ttccttcctc gggtccacga acgggaaggg
      601 aacgaccgtc gcctgtcggt ctcccacgtc cgcgacgctg accgtgagcc cggtcccggg
      661 gctggcgcag tcgatcggga ccatcgcgta gccgatgttc ttctggagcc gcggcgagtg
      721 gatggccgag gtgacctggc cggcgggacg gccgtcgcgc tgcaccggcc actttgtggt
      781 gttgaacgcg atgggcgcac cggcgatctc gaggccgacg agccggcgct gcacgcccgc
      841 cgctttgatg cgtcgcaatg cgtccctgcc gacgtagtcc tgcgtcttgc cctcatctac
      901 gaggcgctgg agaccgacct cgtacggatt gtttgccagc gtcatgtcgg cgccccagtt
      961 caggatcccg gcctcgatgc gccggatgtc ggagggtccg gtggggcgga tgtcgtacgg
     1021 tcgcccggcc gccatcacgc gctcccacag ctcctcgccc cggctgccgt cgcgcaggta
     1081 gatctcgtaa cccacctcgc ccgaccatcc ggtacgggtc acgacgacgg ggatgccgtc
     1141 gaggtcggtc tggaggaacc agtagtacgg cagctgcagc gcgccggcgc cgaacagggc
     1201 ctcgatcacc tggcgggact ggggaccctg gacttgcagc ggtgacacgt cgggctcggt
     1261 gatctcgacc ttcagtcctg agttgagcgc gacgcccttg gcccacagca acacgtcgct
     1321 gtcggccagc gcgagccaga agtgattctc gcccagccgc atcagcacgg gatcgttgat
     1381 gatcccgccg tcggcggcgg tgatgaccac atacttcccc tgaccgaccg cgcagcggct
     1441 caggtcgcgc ggggtgagca ggttggtgaa gtggaacgcg tcgggccccg tgatttcgac
     1501 ctgccgctcg accgcgacat cccacagcgt cacatgacgc agcaggtgcc agtactcctc
     1561 caccatgtcc ccgtaacaaa tggggaagag catgtggttg tagacggtgt acccgcgggc
     1621 gccggcccgc tgggtggcgg caaagtacgg cgtgcggcgc aagcgggcgc cccgctgtac
     1681 cagagccatg tcgaaggtca tcggcgttcc tcgatgcgag tgaatgctgg cgcaccagcg
     1741 caacggcggg aatatgccga gcgcagcggg agtgcgccgg gtgtccggta cggccgccgg
     1801 ggggctactc ttagggcaga atgaccacgc gggaccgcag cgccgcacgc ggcacggcct
     1861 ggcatacgcg cacggtcgcg gaggcgagcc ggctgctcga cgtggagccg ggcgccggat
     1921 tgtcttcccg ccgggcggcg gaacggctgg ccgtgcacgg cccgaatgcg ctcgcggccg
     1981 cgcgccggcg cggcgcgctg cgcgtgctgc tggcgcagtt caccgacttc atggtgctcg
     2041 tcctgatcgc cgccgccgtg gtcgccggcc tcctcggcga gccgcaggac atcatcgcga
     2101 tcgtcgccat cgtggtgctg aacgccgcct tgggcttcgc gcaggagtac cgggccgagc
     2161 gggccatggc tgcactcacg gcgctggcgg ttccgcaggc gcaggtgcgg cgcgacggcg
     2221 tggtgcgctc gctccccgcg cccgagctgg tgcctggcga cgtcgtcctg ctcgaagccg
     2281 gcaacctggt tccggcggat ctgcgactgg tcgaggcggt tcgcctgcga gtcgaggagg
     2341 cggtgctcac cggcgagtcc catccggtcg acaagacgac cgcgcccctg gccgacccgg
     2401 acttgccgct cggcgaccgc agcaacatgg cctaccaggg gacgctggcg acgtccggcc
     2461 gcggcgtcgg catcgtgacg gccaccggga tgacgacgga gctgggtcgc gtggccggcc
     2521 tgctgaagga tgtggaagtg gcccggacgc ccttgcagcg gcgtctcgcg cagcttggcg
     2581 cccggctcac gatcgcgatc gtgatcctgt gcgccgtcat gttcgggttc ggcctgctcc
     2641 gcggcgagga ccccggtttc atgtttctca ccgccctcag cctggcgatc gccggcatgc
     2701 cggaagccct ccccgcggtg gtcacgatct ccctggcgct gggtgcgcgc cgcatggcga
     2761 agcgcaacgc gctggtgcgg cggttgccgg cggtcgagac gctggggtcg gtcacctaca
     2821 tctgctccga caagacgggg acgctcaccg agaatcgcat gcgggtggag gcatatcata
     2881 cgggcgacgg acggatgccc gagccgggcg ggtcggcgga acgtgagccc tggtcgctgc
     2941 tactcaccgc gctggccttg tgcaacgacg cgatccgcgg cccggatcac gacgcccagg
     3001 gcgatccgac cgaggtggcg ctgctcctcg cggccgcacg agccgggcgc gagaaggcag
     3061 cgctggaaga gcggtatccg cgccgcgggg aaatcccgtt ttcccccgag cgcgcccgca
     3121 tgacgacgct gcaccacgac ggggccgagg ttctcgcgtt caccaagggg tcgccggagc
     3181 aggtgctcgc gctgtgccgc gaccgcctca cggccgcggg ccgcgtgccg ctcgcgcgcg
     3241 aggccgtcct ggaggaggcg gccgacatgg cgcgggacgg actgcgggtg ctcgccgtcg
     3301 ccgtgcgccg cctgcagggc gacccggccg cgctttcttc cgaggcgatc gaagcggagg
     3361 agacgttgct cggcctcgtc ggcctgctcg acccgccgcg gcccgaagcc cgggacgcgg
     3421 tcgcgcagtg ccaggccgcc gggatccgcg tggtgatgat cacgggcgac catcccgcga
     3481 cggcccaggc cattgcccgc tcgctcggca tcgcccgcgg cgcggacgag tgcatgaccg
     3541 gccgggagct cgcgacggta ccggccgcgg agctcgcgag tcgggtcgcg ggggtgcggg
     3601 tgtacgcccg ggtggcgccc gaacagaaga tcacgatcgt ccaggcgctg caggaccgcg
     3661 gcgaattcgt cgccatgaac ggggatggcg tgaacgacgc gccggcgctg cgccgggccg
     3721 acatcggggt cgcgatggga cgcagcggca ccgacgcggc gcgcgaagcg gcacacatga
     3781 ttctgctcga cgacaacttc gccacgatcg tcggcgccgt tcgcgagggc cgccgcatct
     3841 acgacaatct gcgcaagttc gtcaaataca ccctcaccac cggggtcgcc gagatctggc
     3901 ccctggtcct ggcgccgatc ctggggatcc cgattcccct gctgccgatt cagatcctct
     3961 gggtcaacct cgtgaccgac ggccttcccg gcctggcgct cgcggcggaa cggtccgagg
     4021 cgcgcgtgat ggcgcggcca ccgcggccgc cccgcgaggg cctcttcgcg cgcggcctgt
     4081 ggcagcacgt ggtgtgggtc gggtttctga tgggcagcat caccctgggc acgcagctct
     4141 gggggtccca agccggcctt cactggcgca gcatgacgtt ctcggtcctg gctctctcgc
     4201 agatgggcca cgtgctggcc atccgctcgg aggacacctc gctgttccgg ctggggctct
     4261 ggtcgaatcc tccgttgctc ggcgccgtcg tggtgacgct ggtgctgcag ctcgcgattc
     4321 tgtacgtgcc ggggctgaac acgctgttcg gcacacagcc gctcaccggg ttcgagctgc
     4381 tcgttacgct ggcgctttcc tcgatagtct tcgtggcggt ggagatcgag aagtgggtga
     4441 agcggaaagg gggacttcgt gtttcgcaag gcgatcgtcg tcggggttga cggctcgtcg
     4501 gaatcgctgc gcgccgcggc ggtggcgcgt cgcatcgccg aagcggtggg gggaggcgtc
     4561 cggctgcagg tcacgcacgc cgtgccggaa gcgagactgg cgaaggcggt gggcccggtc
     4621 cccgtgtacg tcagcgggct cttcgcgcgg gcgctggcgg cgacccgccg agacctcgcc
     4681 ggcaggctgc ggggcgtggt gccgccggcc gcgctcgaga cgctcgatgt ccgcgccggc
     4741 agggcggcgc aggtgctggc ggacagcgtg gcccggcgtc gcgccggcct ggtcgtgctg
     4801 ggcggcaagc ggcacggcat actggcccgc acggtgcgcg gcagcaccgc gcactacctc
     4861 gcgcgcgtac tcgacatccc gattctcgtg acgggcgctg ggggacgcgc cctcggacgc
     4921 gtgctcgtgg cggtcgacct ctcgacggcg ggcggaagga cgctcgccgc gggcgtgggg
     4981 ctcgcgcggg cgcttggcgc gcgggtgcgc gtgctgcacg tcgtggagcc gatccgctac
     5041 gcctacgtga tgttgcgcgc gccggatgcg aaggccgttc tcgagcggtc ccgcgcggcg
     5101 ctcctgcgcc tggtggcgcg gtacgggatt gcggggaccg acaccgtcgt gcgccggggt
     5161 gccgcgctcg gcgtgatcgc ctccgaagcc gatgagtggc gcgccgatct gatcgtggtc
     5221 gggtcgcagg ggaagggttt cgtagaccgt ctgttgatcg gcagcacaac ggaatcgctg
     5281 ctcgcacgcc tgccggcgtc gctgctcatc gtccccgtcg cggggaggaa atcgcggcgg
     5341 ctacgcgcga cgcggtgacc atccctcccc ccgggcgagg ctccacttca gggcggggtg
     5401 atcatggtcg tcacgataac catgatcacg attgccgagt tagatgctgg aatccacgat
     5461 cggcgcgccg gccagcgtct gcgtcatccc gatgctcgcg aagatcagtc cgaccttccc
     5521 gcgcgggatc atgccgatgc cgatcgagcg ccggtccagt ggccggccga gcgtacccat
     5581 ggagcacgcc tgcttgcggc ggccgactcg gcaagcaaga cgcgcggcgc tatgttggct
     5641 gggacgacga ttcgacgggg aggttgccat ggggctcatc gcgtcgttgt gtcgggcgag
     5701 cggcgcggca atcggttgca ccctgatgtt gtacgcggcg ctggatgccc agcggccgag
     5761 cgaagagccc aagcggccct ctatggaggc gacgcgcgac accaacagcg gcgcttccta
     5821 ttacctctac gggctctcgc aattgcgcag taatcccacg cgggccgccg ccgcgttcta
     5881 ctgggccacg cggctcgagc cggacatggc cgagccgtgg tacggacgct gggcggcact
     5941 gctgctctcc gaagacccgc gtgtcttggg cgactacctc gccgggaagc cgtacatcgc
     6001 caagtcgcgc gacatccagc agatcgattc gttgcgctac caggcgatga tccgtgagcc
     6061 gctgctgcac tggggactgg atcagatcct gctcgacgaa tggctgtcgc gcgtgagcgg
     6121 gggcgaagtg agcctcaaca tggtggtccg cgactacggg ccggagatgg cgggctggtg
     6181 ggcgtacggg cagggccagt tcctcgaggc gctgaagcag tacgccgtcg cggtgaagca
     6241 gaatccgaag tcctacgccc tgcgtctggc gcgcgcgcgg tcgttcctgc ctctcctcca
     6301 attcgacagc gccgcggcgg cctacgccga ggtgctcacg ctacagagcg gccaggaggc
     6361 agagcgcgta gtgcacgtct acgacagcaa ggagatcgcc tactacacga tcggccggat
     6421 tcgcgagacg cagcgcaacg tcgccgcggc gcgggaggcg tacgggcagg cgctcgtgga
     6481 aaacctggcg ttctacccgg cccacgccgc tctggcgcgc gtggcgcttg cagtggggga
     6541 cagcgcggca gcggagcacg agtacgacca ggccctgcag ctccacccgg acaatccggg
     6601 agctcactac gactacggcg tgctgttgta cgccctgaag cgttacgagg cagcggccga
     6661 gcagctccag ctggcggtag atgaggaccc ctattacgcc agaccgtact ttctcctcgc
     6721 ctacctgcgg caatacgaag gggaagactc ggtcgccacc gccttgtacc ggcgcttcat
     6781 ccagatcgcc cccgcctcgc aggccgccca ggtgaagttg gcgcgggaac tgctgggcga
     6841 gcaggccgcg ccaccgcgtt gagcatggca gtacgagtcc tcctgtatcg tgcccggcat
     6901 actgctcgac gacgtgtcga tccggcggcc gagaggtgag gtatccaagc cgccggtgct
     6961 gaccccgtcc tgggccggcg gaccatagca ggacgtgaat cgtcatagca ctcgtcgcgg
     7021 tggtcgcggg gccttccagc gggcactcgc gcggttagct tacgggcgcg cgcacccggt
     7081 cggccgaacg cccaaatccc catgaccaat cccgagcacg acctcgcaaa gctccacatc
     7141 gaccgcgatg cgccacccgc cgtgcggcgg gcattcaccc gcaatctcct gctggcggcc
     7201 gccgcggtcg cgctgctggg tggtggggta ctcttcgccc ggcgcggcgg ggcggtcccg
     7261 gtacaggtgg tgatcgcgac ggcggcgagc ggtggtggcg gcgccgcggg caacgccagc
     7321 gtgacggcga acggctacgt ggtcgcacga acgcgcgcct cggtctcctc caaggtcgcc
     7381 gggcggctcg cctacctcgg tgggagcgaa gggtcgttcg tgcgcaaagg cgacatcatc
     7441 gcccggctcg aaaacgcgga ttatcgggcc gcagtggccc aggcgcaggc ggcggtagcg
     7501 gcggcgcgag ccgattcgat cgaaagcacg gcggaccgcg accagatgga gcgagaggcc
     7561 cggcggctcc gcaccatcca tgcgcagaac cgggagctgg tggcgcaaca ggaagtcgac
     7621 caggccgaga gccgcgcggc gcagagcgcg gcgcgggcgc aagccaccgc ggcgcgggtc
     7681 gaagcggccc tcgccgggtt gcggttcgcg caggcgaatc tcgacaacac ggtgattcgc
     7741 gcgcccttca ccgggacggt gctgcgcaag gatgcggagg tcggggaggt cgtggcgccg
     7801 tcggtgggcg ggggcctcac gcgtggcgcc gtggtgacca tggccgatct cgccacgctc
     7861 gaagtcgagg tggacgtgaa cgaagcctac atcgcgcgga ttctcgggcg ccagccggcg
     7921 cggatcacgc tcgacgccta tcccgatacc ggtttccggg gcgcggtgcg ccaagtggtg
     7981 ccgacggccg atcgccagcg ggcgacggtg caggtcaagg tcagcatcac cgaccgcgat
     8041 ccccgcatcc ttccggaaat gggcgcgcgg gtcgactttc tcgagcccga cacggcccac
     8101 gcgcccgtcg ccggggccac ggcgcctccc cggatccgcg ttcccgcggc cgccgtgcgg
     8161 gacgaaaacg gcgagagcgt cgtctggctg gtgcggcagg gccggctcga gcggcgcgcc
     8221 gtgcaggccg gaccggtcag tggcggcttt cgcgagatcc gcagtggcct ggtcggtggg
     8281 gaacttctgc tggcgggcgg cgttgaaact ccccggcccg gcatgcgcgt caagacggcg
     8341 acaccctgac acgggacacc tccatggccc tcgccgaaat ccgcgacgtc cacaagtcct
     8401 tccaacgcga cgcacagcgc atcgacgtgt tcacggggct gacgctcgac atccccgaag
     8461 ggagcttcac cgccctgatg ggaccgtcgg gctccggcaa gtccacgctg ttgaatctgc
     8521 tcgccgggct cgaccgtccc accagcggaa cggtgcgcgt tgcgggcgcg caggtcagcg
     8581 cgatgacccc ggcccagctg gccacgtggc gcgcccgaca catcgggttc gtatttcagt
     8641 cgttcaacct gctgccggtg ctcaccgcgt atcagaacgt cgagctgccg ctcctgctca
     8701 cccgactctc gcgcaagcag cgcgacgagc gggtgcgcgt tgcgctcggc gtcgtgggcc
     8761 tggacgaccg cctggaccac tatccccggc agctgtcggg tggccaggag cagcgcgtcg
     8821 cgatcgcgcg cgccatcgtg gcggacccga ccctgatcct gctggacgag cccaccgggc
     8881 agctcgacgc gaagagctcg caggaagtgc tcggcctgct ggggcggctg aacgacgagt
     8941 tcaagaagac catcgtcatc gtcacacacg atgcgcacgc ggcggagcgg gccaagaccg
     9001 tgctgcacct cgagaagggg cagctgatcg agatggcggg agcgtcgcca tgaagtactt
     9061 cccgctggtg ctggcgaatc tcggccgcca caagctgcgc acggtgctca ccatcgccag
     9121 cgtcgccctg gcgctgttcc tgttcgcctc gctgcgcacc gtcgtcacca cgctgggcgc
     9181 ggcggcgcag ttcggcagcg cgcgccgcct cgtggtcacc aacgccaccg ggatcgtctt
     9241 cccgctgccg ctcgcgtacg cgaaccggct gagcaccgtc tccggggtgg agggggtgag
     9301 ctgggccaac tggttcggcg ggcggtacgg cgacggcaag cgattcttcg ccaacttcgc
     9361 ggtggacgtc gaatcctatc tcgacctgta tccggagatc cagctgccgc ccgagcagcg
     9421 cgcggcgttt ctccaggatc gctcctcggc gctcgtcggc cgggggctgc tcaacgtgtt
     9481 cggctggcac ctgggccaga acgtcacgct ccaggggacg atcttccccg gcgactggac
     9541 gttcacgatc cgcggcgtgt acacgccctc ggaccgcgcg atcaacgacg acatgctgct
     9601 gttccaccac gattacttcg acgagcggat cgggcgcgcg gggatcgccg gatggtacgt
     9661 cgtacgcatc aacgacccca accgggccgc gcagatcgcg aagaccatcg acgaccagtt
     9721 ccgcaattct agctccccca ccaagaccgg gaccgagcag gcgttcaacg ccagcttcgc
     9781 cacgatgtgg ggcaacgtac gactcctcat ggggacgatc ggcatcgcgg tggtgttcgc
     9841 catcctgctc gtcaccgcca atgccatgat gatgagcgcg cgcgagcgga ccggcgaaat
     9901 tgcggtgctg aagacgatcg gctaccccga ccgcacgctg ttcgggctgg tgctgctcga
     9961 ggcgggagtc gtgaccctca cgggagccct catcggcctg ggtggcgcga agctgctgta
    10021 ccgtgccacc aacttcaacg ccgccgggtt cctgcccggg ttcgacgtca ccaggagcac
    10081 gctcgttctg ggcgcagccg tggcactgct cctgatgctc gccagcgggc tcgtccccgc
    10141 cgtgcgcgcg gcccggctgc cggtcattca agccctgcgg cacgtcgaat gagcatcccg
    10201 ctcatctaca acgtccgcag cgtgctgcag cgccgggtct cgacggggtt gaccgcactg
    10261 ggcattggcc tggtggtcgc cgtgttcatc ggcaccctgg cgctggcgag cggcttccgg
    10321 gcggcgctgg tgcggacggg ttcgggcggc aacgtcctgg tgctgcggcg cggcgccgat
    10381 tccgagctgg cgagcggcct gtcgcgtgaa acggcgagca tcatcgcggc gtctccccac
    10441 gtcgccaccg ggccggacgg gcggccgttg gtgagccccg aggtgtacgt gctggtgccg
    10501 ctgccccggc ccacagacac gacggcggtg ggcaacgtcg tggcacgcgg ggtgagcgac
    10561 caggcgtggg tggtccggac cggcgtgcag atcgtcgcag gccaccggcc ccaatcgggg
    10621 cgcgccgaga tctgcgtcgg ccggcg
//
LOCUS       DBNGZW010000125         2250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1442881, whole genome shotgun sequence.
ACCESSION   DBNGZW010000125 DBNGZW010000000
VERSION     DBNGZW010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1442881"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..281
                     /locus_tag="AC16JC_03090"
     CDS             <1..281
                     /locus_tag="AC16JC_03090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163711.1"
                     /translation="TPVWLSVRDGVGHELPKGRLGPQGRVQFLWPFIDLNSGVFIATT
                     QEGRVTMSDAFKPWGGPKAWGWDVSNPTPQSASHSFCPQPAGDVAAGN"
     gene            350..667
                     /locus_tag="AC16JC_03095"
     CDS             350..667
                     /locus_tag="AC16JC_03095"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR5163712.1"
                     /translation="MADLSSLFQLAQQAQGRLSEIQTRLAQETVSVSSGGGMVQATAD
                     GRGQIRAIKIDPEAFKGGDVELLEDLVLAAVTQAQQRAAELYQAEVKKLTAGLPLPFQ
                     LPL"
     gene            699..1298
                     /gene="recR"
                     /locus_tag="AC16JC_03100"
     CDS             699..1298
                     /gene="recR"
                     /locus_tag="AC16JC_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020391097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR5163713.1"
                     /translation="MDELTAELAKLPGIGRKTAQRLAFHLLKEPPETARRLAEAIRRV
                     RDQVQVCTTCGNLGDDSPCAICRDPRRDATVLCVVEEAGDVSAIERAGRFRGLYHVLG
                     GRLSPLEGVGPEALQIDRLLERVSNGGAAGVQEVIVATNPSMEGEVTATYLQQVLKPS
                     GIRVTRIARGLPVGGDLEYADGVTIAQALAARRDMTETD"
     gene            1310..1795
                     /locus_tag="AC16JC_03105"
     CDS             1310..1795
                     /locus_tag="AC16JC_03105"
                     /inference="COORDINATES: protein motif:HMM:NF015232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HIR5163714.1"
                     /translation="MTGAASWSFNESDYRQIQQHLQAFLRDSNARCALLVDRTGQLVA
                     TVGEPPAFDPTAFASLTAADFSANDQLAKMIGEPEFASLVHQGEKESMYLADVARRVI
                     LVVLFDQRATLGLVKLKAKATVEALNHVFQIMFDRVGAGPGEAKGLLQGAEDEIDKLF
                     G"
     gene            1811..>2250
                     /locus_tag="AC16JC_03110"
     CDS             1811..>2250
                     /locus_tag="AC16JC_03110"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR5163715.1"
                     /translation="MSMINYASREINCKLVYYGPGLGGKTTNLEHIYGKVAPNTRGKM
                     ISLATETERTLFFDFLPVDLGTIRGFKTRFHLYTVPGQVYYNASRKLILKGVDGIVFV
                     ADSQVERMEANVESMQNLYDNMSEYGYDLTKIPFIIQYNKRDLP"
BASE COUNT          378 a          743 c          797 g          332 t
ORIGIN      
        1 acacgccggt gtggctgtcg gtgcgcgacg gcgtagggca cgagctgccc aagggccggc
       61 tgggaccgca gggccgagtg cagttcctct ggccgttcat cgatctcaat tccggcgtgt
      121 tcatcgccac cacgcaggaa ggccgcgtga cgatgagcga tgccttcaag ccgtggggcg
      181 ggcccaaggc gtggggctgg gatgtgtcga accccacccc ccaatccgcg tcgcattcct
      241 tctgcccgca gccggcgggg gacgtcgcgg cggggaactg aaggcgaggc ggcccggcgc
      301 gcagtcggtc agcttgcgac ccgattgcgc gccgggtaac ttttgaccca tggcggattt
      361 atccagtctg ttccagctgg cccagcaggc ccagggccgg ctgtccgaga tacaaacgag
      421 gctcgcgcag gagaccgtct cggtgtcgtc gggcggcggc atggtgcagg ccacggccga
      481 cgggcgcggc cagatccgcg ccatcaagat cgaccccgag gcgttcaagg ggggcgacgt
      541 cgagctgctg gaggacctgg tgctggcggc cgtgacccag gcgcagcagc gcgccgcgga
      601 gctgtaccag gcggaggtga agaagctcac cgccggactg ccgcttccgt tccagctgcc
      661 gctgtaagcc tcccccgtgt ccgcgcccag tgtccgcgat tgacgagttg accgcggagc
      721 tcgccaagct ccccggtatc gggcggaaaa cggcccagcg gctcgcgttc cacctgttga
      781 aggagccccc cgagaccgcc cgccggctgg cggaggcgat ccgccgcgtg cgcgaccagg
      841 tgcaggtctg cacaacgtgc ggcaacctgg gcgacgacag cccgtgcgcc atctgccgcg
      901 acccgcggcg ggacgcgacg gtgctgtgtg tcgtggagga ggcgggggac gtgagcgcga
      961 tcgagcgcgc cgggcgcttc cgcggcctct atcatgtgct gggcgggcgg ctgtcgccgc
     1021 tcgagggcgt gggccccgag gcgctgcaga tcgaccggct gctggagcgg gtgtccaacg
     1081 ggggcgccgc cggggtgcag gaggtgatcg tcgccaccaa tccctccatg gagggggagg
     1141 tcaccgccac ctacctgcag caggtgctga agccttcggg gatccgcgtc acgcgcatcg
     1201 cgcgaggact cccggtgggc ggcgatctgg agtacgccga cggggtcacc atcgcgcagg
     1261 cgctcgccgc ccgccgcgac atgaccgaga ccgactgagc gagggcgcga tgacgggtgc
     1321 cgcgagctgg tcgttcaacg agagcgacta ccgccagatc cagcagcacc tgcaggcgtt
     1381 cctgcgcgac tcgaacgccc gctgcgcgct gctggtggac cgcaccggcc agctggtcgc
     1441 cacggtgggg gagccgcccg ccttcgatcc caccgccttc gcctcgctca ccgccgcgga
     1501 tttttccgcc aacgaccagc tcgcgaagat gatcggcgag cccgagttcg cgtcgctcgt
     1561 gcaccagggc gagaaggaat cgatgtacct ggcggacgtg gcgcgccgcg tgatcctggt
     1621 ggtgctgttc gaccagcgcg ccaccctggg gctcgtgaag ctcaaggcga aggcgaccgt
     1681 cgaggcgttg aaccacgtct tccagatcat gttcgaccgc gtgggcgcgg gaccgggcga
     1741 ggccaagggg ctgctgcagg gggcggaaga cgaaattgac aaactcttcg ggtaggcggg
     1801 agacgcgtcc atgtcgatga tcaactacgc gtcccgcgag atcaactgca agctggtgta
     1861 ctacgggccg ggactgggcg gcaagacgac caatctggag cacatctacg ggaaggtcgc
     1921 gcccaacacc cgcggcaaga tgatctcgct cgccaccgag accgagcgca cgctgttctt
     1981 cgatttcctc ccggtggacc tggggacgat ccgcggcttc aagacgcgct tccacctcta
     2041 caccgtgccg gggcaggtgt actacaacgc gagccgcaag ctgatcctga agggcgtgga
     2101 cggcatcgtg ttcgtcgccg acagccaggt cgagcgcatg gaggccaacg tcgagtcgat
     2161 gcagaacctg tacgacaaca tgagcgaata cgggtacgac ctgaccaaga ttccgttcat
     2221 catccagtac aacaagcgcg acctgcccaa
//
LOCUS       DBNGZW010000126         9939 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_76469, whole genome shotgun sequence.
ACCESSION   DBNGZW010000126 DBNGZW010000000
VERSION     DBNGZW010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9939)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9939
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_76469"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /gene="nadC"
                     /locus_tag="AC16JC_03115"
     CDS             complement(<1..762)
                     /gene="nadC"
                     /locus_tag="AC16JC_03115"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR5163716.1"
                     /translation="MAEPFGREEVRRALAEDWATQDVTTALLGPAARRPATAEFVAEG
                     RFVVAGLVIVASVYEELDRDARIEALCGEGQWTSAGAVLARVRGAAGTLLAGERVALN
                     FLQRLSGVATVTRRAVDAVAGTGARITHTRKTTPGLRTLERYAVTAGGGVENRGSLAD
                     AVLWKDNHWVLLGAGGLAAALARAAPGTPVVVEVETDAQLDEALAAGVRHLLVDNQTP
                     ERVAAWVRRAGRGVTVHASGGITHQTARAYADAGAH"
     gene            complement(755..1708)
                     /gene="nadA"
                     /locus_tag="AC16JC_03120"
     CDS             complement(755..1708)
                     /gene="nadA"
                     /locus_tag="AC16JC_03120"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008482038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR5163717.1"
                     /translation="MTAAATDLIAEIRELKAERRAAILAHNYQRPEVQDVADLVADSL
                     RMARRATELEAPLLVICGVHFMAETAAIANPAKRVLIPDLDAGCSLADTIRPEDVRAW
                     RAAHPDGLVVTYVNSSAAVKAESDYCCTSGNAVAVVQALPPGVPVLFVPDFFLGNHVR
                     RVTGRDIEVWLGECHVHAALRPQAVAARRRELPDAEFLVHPECGCTTSAMYDAVADGD
                     VAAGRSHIVSTEQMMQRARTSPSRRFVVATETGVLHRLRRENPAKEFHALVESAECRF
                     MKRITLENLRDALRDLKYEVTVPPDVAARARRAIDRMLAIG"
     gene            complement(1992..2486)
                     /locus_tag="AC16JC_03125"
     CDS             complement(1992..2486)
                     /locus_tag="AC16JC_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163718.1"
                     /translation="MRFFTRTCGTATLLSGLVLFGFSTAAAQRPTHAQAPTPHVRSAP
                     RSAASPRASISEPKVTSFRGIASKLNTTPDALESAYQTARAANPKLTRGQFTSANVLA
                     QNLGTKDPSITTQAILDGLQSGKSIGKTLQGLGLSETEADKAQQEANSEIRRAGQQPR
                     DSTK"
     gene            2650..3495
                     /locus_tag="AC16JC_03130"
     CDS             2650..3495
                     /locus_tag="AC16JC_03130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163719.1"
                     /translation="MRAQLGSLALFASGVGASLSGQSAVGAGITTGTARFNSARSEQA
                     VTATVQVRPRPWLSLSAIPSVVRVSDTVAGQNVSHSGLGDLPLVVGADHAFAGPWSPV
                     VGVALVATLPVGNASCGLGSGEMGFGPDGGIGVAPGARVRLSASASRSLGGLGAQSAL
                     SAPRATVLRVESAYDVTARWTADLALGADVGSSDSMQALSRVIGAGASYPIAGALVLT
                     VDATRGLTTASPQWVFSVGLGTVFVGTSPVSPTSPLRRLKTGFMSGVSRGGGRGTGRT
                     VAGGC"
     gene            complement(3626..6691)
                     /locus_tag="AC16JC_03135"
     CDS             complement(3626..6691)
                     /locus_tag="AC16JC_03135"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR5163720.1"
                     /translation="MTSAAIQGRVVEPDGRPIAGAIVAIISVPNGQQWRVSTASSGVF
                     ALENVPVGGPYSIVVRALGFAAVRLAIPALALGQRYVADLVLQPAAIQLEPLTVAATA
                     APLINWGYTGPTQLISDSAVARFPNLAHSLANLALLSPYVSLRSGGAMSIGAQNPVLN
                     RFQIDGGASGEAYLGREPGGGIPAAAVPQVLPRTISMEAVQEFQVLVAPFDVRQGGFA
                     GGLVNVVTRSGTNTSHGSVFTFFENQSFAARPTARAGAEDFAIWQVGGWAAGPVVRDR
                     LQYFASADLHQRVVPDPGPLIADTAGGADTTRIGIRYASAVRFDSILRRTYGLDPGTL
                     GPSEGRIPTADLFGKVTAQLGTNSRVELSHHYAHGSRRGFVPRELGIYRLSSVAEEDA
                     STQSSTRLVWATVLAGRHANELQVSYLSLRDPCRPNADFPRIQVSADNGRLFAGPNMV
                     CPTNDVSQEVLELTDNLTITAGAHLITLGTHNELLHFRDPLLLESAGTWEFSSLDKLD
                     SAQASSYEIGLPGTDRRVVDFHVRQVGLYTQDRWTPVARLALTLGLRIDVPVLPDAAR
                     TNSTLKDSLGINTAQLPSGTPLWSPRLAAAYDLSGDGSMLVRGGIGLFSGSPPYRYLG
                     NAYRYDGLEERELKCDSPYVPPFSPMTRPTSCVIGGRQPVPRLSFFDPGLTFPQSLRL
                     SVGLDRRLPSGVVGTVEVLYTRAVHDWYFTDANLQPPVGAAFGEGGRPLYGTIGSVGH
                     PAYADSLYGPIIRVSNGRGGRTVSFSVQLQRQFRNGIAFSAAYAYSRAEDRMSLVNYA
                     AIPQVAGTPLDGTLEDRRLRRSSFDIPHRVSVSVSANLPYHTQLALIYTGSSGTPFTY
                     VINGDANADGMGDPPAVLRNDIVYVPRDSGDITLASGQSRTALDAFINANPCLRSQRG
                     RIMTRNSCRNGWLGYFNLRLAKTVSPSAGHRGAEFAADILNILNLLSSRWGRYRATAP
                     TDAPTVTLLKLVGYDAVNGRGVYKVTLAPNQVVDAASRWQVQLGVRYRF"
     gene            complement(6768..9365)
                     /locus_tag="AC16JC_03140"
     CDS             complement(6768..9365)
                     /locus_tag="AC16JC_03140"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR5163721.1"
                     /translation="MAAAIEFRILGPVSLRDGDGREAGGLLAQPRRLALLAYLAAATP
                     RGLQRRDVILTLFWPELDQEHARAALRQALRVIRGALGAVVVSRGDDEIGLDFGRVSC
                     DVVSFESALAAGRLDEALELYRGDLLVGFFIAHAPEFEEWMEKQRVRLRTVAAAGARR
                     RAEELQAAGDLVSAVTTYRRALELAPHDEILVRRTIELLDRLGDRAGAAQLYETFERR
                     LVADLGVEPAPETKALMAAVRGRQSASAPLEPAGPPIPQGHQAPATATAPLTPSPSRA
                     RSGRLAWPIGVAAAIGLAAVGVVAGARRHAAPDLRSNRVLIAPLDNRTGDTTLSALGQ
                     LAAEWIARSLAQTGLVEVADPASERLARGPAGGGGPTAPSPGAAEGARRLALESGSGL
                     AVWGDYYRRGDSVELEAQITDERRRTLLRSVGPVTASLADPRPAIAALAQRVTGALGT
                     ALDPKLRDWAGLASQPPSYEAYREFMAGVHAWYDQLKAREGLEHFYRAAALDSSFTLP
                     LVWAAWGHAFLRECAITDSLGRVLHARSPYAGRIDLLHVDLEVAICRGDQEAAYRLSH
                     ELWEAAPASEMWATVAARQALVMNRPREAIAILEGLHPDRGALRGHRPYYIWLTTAYH
                     RVGDYAHELAAARRARQQYPNDLTMLRRELSALAALGRTREVNEGLEELAAMAAHPLG
                     RTPAEVMLWTAAELQLHGSSMADSLLMQALRWQLSRPPEEQATEIGQLNLAQTFAFMG
                     RVQEAYPIAERLAREHPGNERYVGLLGVLAARRGERGTAEHSERALAEDRGPYDQVNR
                     TYWRACIAVQLGQRQQALDLLRQALTEGALGRTLVGYEPHGPPMDIDPFLAPLRQDSA
                     FVTLLKAKG"
     gene            9501..>9939
                     /locus_tag="AC16JC_03145"
     CDS             9501..>9939
                     /locus_tag="AC16JC_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163722.1"
                     /translation="MNARRKLTAAAALSALAVACNVETESVGPTLSVFDLQSEASSAP
                     LVLHLVAPQATDPAIDQFLADHYVWLDTAAHPNHKLFVLMPGTGQIPAAFQFVEREAA
                     RLGYAVIGLSYLNSGGFAKVCPLATDPAACYEDSRLEIVDGIER"
BASE COUNT         1557 a         3383 c         3462 g         1537 t
ORIGIN      
        1 gtgcgcgccg gcatccgcgt aggcgcgcgc agtctggtgg gtgatgccgc ccgaagcatg
       61 gaccgtcacg ccgcggccag cgcgccgcac ccaggccgcc acccgctcgg gtgtctgatt
      121 gtcgaccagg aggtggcgca cccccgcggc cagcgcctcg tcgagctggg cgtcggtctc
      181 cacttcgacc acgaccggtg taccgggcgc cgcccgcgcc agcgccgcag cgagtccccc
      241 cgccccgagc aatacccagt gattgtcctt ccagaggacc gcgtcggcga gcgagccacg
      301 attctcgact ccgccgccgg cggtcacggc gtagcgctcg agcgtccgca gaccgggggt
      361 ggtcttgcgg gtgtgcgtga tgcgggcgcc ggtgcccgcg accgcgtcca cggctcgccg
      421 cgtcaccgtg gcgacgcccg agagccgttg caggaagttg agcgcgacgc gctcgccggc
      481 gagcagggtg cccgcggcgc cccgcacccg ggccaggacg gcaccggcgg aggtccactg
      541 cccttccccg cacagcgctt cgatgcgcgc gtcacggtcg agctcctcgt agacgctcgc
      601 cacgatcacc agccccgcga cgacgaagcg gccttcggcg acgaactcgg cggtggccgg
      661 ccgtcgcgcc gccgggccga gcaacgcggt cgtcacgtct tgggtggccc agtcctccgc
      721 cagcgcgcgt cgcacctcct cgcggccgaa cggctcagcc aatcgccagc atccggtcaa
      781 tcgcgcgtcg ggcccgcgcg gccacgtcgg gcggcacggt gacctcgtac ttcaggtcgc
      841 gcaacgcatc gcgcagattc tcaagtgtga tccgcttcat gaagcggcac tccgccgact
      901 cgacgagcgc atggaattcc ttggcggggt tctcgcggcg cagccggtgc agcacgcccg
      961 tctccgtcgc caccacgaag cggcggctgg gagaggtgcg cgcccgctgc atcatctgct
     1021 cggtggagac gatgtgggag cggcccgcgg ccacgtcgcc gtccgccacg gcgtcgtaca
     1081 tcgcgctcgt ggtgcagccg cattccggat gcaccaggaa ttcggcgtcg ggcagctcgc
     1141 ggcgccgggc ggccacggcc tggggtctga gcgccgcgtg cacgtggcac tcgcccagcc
     1201 agacctcgat atcgcgcccg gtcacccggc gcacgtggtt cccgaggaag aaatccggca
     1261 cgaacagcac cggcacgccc gggggaagcg cctgcaccac cgccacggcg ttccccgagg
     1321 tgcagcagta gtccgactcg gccttcaccg ccgcgctgga attcacgtac gtcacgacga
     1381 gaccgtcggg gtgggcggcg cgccaggcgc gcacgtcctc cggccggatc gtgtcggcga
     1441 gcgagcaccc agcgtccagg tccggaatga gaacccgttt tgccggattc gcgattgcgg
     1501 ccgtctcggc catgaagtgc acgccgcaga tcacgagcag cggcgcttcc agctcggtgg
     1561 cgcgccgcgc catccggagc gagtccgcga ccaggtccgc aacgtcctgg acttccggcc
     1621 gctgataatt gtgggccagg atcgcggccc ggcgttcggc tttcagctcc cggatttccg
     1681 cgatcaggtc cgtggccgcg gcggtcacgg gttcgtcgag gtgcctctga tgcctttcga
     1741 ggacattgcc acgctcccat tggtggagga accgtcgctg aggggccgtt tgggcaacgc
     1801 ctcggtccac ggaagatgtg gaccgctccc agcggggggc gcccgaactc ttgcgccatc
     1861 tccatactaa tcagaatgag ccgcccgcgc agactccgga cgggctgcga cgctaaccag
     1921 ccccatggtg agcatcgaac gggccggccc gtatctggcc ggcccgtcag gcaggaaggg
     1981 gccgtgtccc gttacttcgt cgagtccctg ggctgttgcc cggcccgacg gatctcgctg
     2041 ttggcctcct gctgcgcctt atccgcctcg gtctcgctca aaccgagccc ctgcagcgtc
     2101 ttcccgatgc tcttgccgga ctgcagtcca tccaggatcg cctgggtcgt gatgctcggg
     2161 tccttcgtac ccaggttctg ggccaggacg ttggccgagg tgaactgccc gcgagtgagt
     2221 ttcgggtttg ccgcccgggc ggtttgatag gcgctctcca gcgcgtccgg agtcgtattc
     2281 agcttcgagg cgatcccgcg gaagctcgtg accttcggct cggaaatcga tgcccggggg
     2341 gacgcggctg accgcggcgc acttctcacg tgcggcgtcg gcgcctgggc gtgagtgggg
     2401 cgttgggccg cggccgtcga gaagccgaag agtacaagac ccgagaggag ggtcgccgtt
     2461 ccgcaggtgc gagtgaagaa cctcattgtg tcctccatgc tgtcggcatg agtgccgttt
     2521 cgtgaattcc taccccgacg agcctaggac ttccccaggc cgtcccgtgt cacggtagtg
     2581 acccagctca ccggaccccg cttttagagg agcgggtagc atcgtgcaac cccacgcacg
     2641 ggagtctcca tgcgcgctca gctcggttcc ctcgctctgt tcgcctcggg tgtcggcgca
     2701 tccctatcgg ggcagtcggc cgtcggcgcg ggcatcacga ccgggaccgc ccgcttcaac
     2761 agcgctcgat ccgaacaggc cgtcaccgcg accgtccagg tacgaccccg accctggctc
     2821 agcctgtcgg cgatcccgtc cgtggtacgc gtgtcggaca cggtcgcagg acagaacgtc
     2881 tcgcacagcg gtctggggga cctcccgctc gttgtcggag cggatcacgc gttcgcgggc
     2941 ccgtggtctc cggttgtcgg cgtcgcactg gttgccacac tgccggtggg caacgcgagc
     3001 tgcggcctgg ggagcgggga gatgggcttc ggtccggacg gcgggatcgg ggtggcgccg
     3061 ggagcacggg tgcggctgtc cgcgagtgcg agccgcagcc tcggcggact gggcgcccag
     3121 tcggcgctga gcgccccgcg tgccaccgtg ctccgggtcg aaagcgccta cgacgtcacc
     3181 gcccggtgga cggccgatct cgcgctgggc gcggacgtgg ggtcgtccga ctcgatgcag
     3241 gcgctgagcc gggtgatcgg agcgggagcc agctacccga ttgccggtgc cctcgtcctc
     3301 acggtcgacg ccacgcgcgg cctcacgacc gcgtcacccc agtgggtgtt ctccgtggga
     3361 ctcggcacgg tcttcgtcgg cacgtctccg gtgagcccca cgtccccgct gcggcgattg
     3421 aagacgggct tcatgagcgg cgtgagcagg ggaggcgggc gcggaacggg aaggacggtg
     3481 gcgggcggtt gttgagcggc ctcaggctcc gaagacgagc caagcgagac cacgaagtcg
     3541 catcccaacc acagctgggg gggttatgca aggctgagca gtaagccagt tcccccatgg
     3601 gcatcaggac cccgaggccc tgcaatcaga acctgtatcg cacccccaac tgcacctgcc
     3661 agcgcgacgc ggcatccacg acctggttcg gagcgagcgt caccttatag acgccccgtc
     3721 cgttcaccgc gtcgtaacct acgagcttca gcagggtcac agtgggcgca tcggtcggag
     3781 cggtcgctcg gtaccgcccc caccgggagc tcaacaagtt gaggatgttg aggatgtccg
     3841 ccgcgaactc cgcgccgcga tgcccggcag acgggctcac cgttttcgcc agccgaaggt
     3901 tgaagtaccc gagccacccg ttgcgacaac tgttccgcgt catgatccgg ccccgttggc
     3961 tgcgcaggca ggggtttgcg ttgatgaagg catcgagcgc cgtccggctc tgaccactgg
     4021 cgagggtgat gtcgcccgag tcgcgcggaa cgtatacgat atcgttcctg agaacggcgg
     4081 gtggatcgcc catcccgtca gcgttcgcgt cgccattgat gacataggtg aagggagtac
     4141 cggaacttcc cgtgtagatg agtgcgagct gcgtgtggta gggcaaattg gcgctgacgc
     4201 tgacagaaac gcggtgcgga atgtcgaagg acgaccgccg cagccggcga tcctccagtg
     4261 tgccgtcgag cggcgtgccg gcgacctgag ggatcgccgc gtagttgacc agcgacatcc
     4321 ggtcctcggc gcgtgagtac gcataggctg cgctgaaggc gatgccgttg cggaactgcc
     4381 gctgcaactg caccgagaac gacaccgtgc gcccaccgcg gccgttcgat acgcggatga
     4441 tgggtccgta gagggagtca gcgtaggcgg ggtggccgac ggagccgatc gtcccgtaga
     4501 ggggacggcc gccctcaccg aacgccgcgc cgaccggagg ctggaggttg gcatcggtga
     4561 agtaccagtc gtgaaccgcg cgggtgtaga ggacctcgac ggttccgacg acacccgacg
     4621 gcagccggcg gtcgagcccg actgagagcc gcaagctctg cggaaatgtg agacccggat
     4681 cgaagaagct gaggcgtggt acgggttgcc gccccccaat cacgcacgag gtcggccggg
     4741 tcatgggact gaagggcgga acgtagggac tatcgcactt caattccctc tcctcgaggc
     4801 cgtcatagcg ataggcgttg ccgaggtagc ggtatggcgg gctgccgctg aacaggccga
     4861 tcccaccccg gacgagcatc gacccgtcac cgctcaggtc gtacgccgcg gcgagacgag
     4921 gcgaccagag cggcgtgccg ctcggcagct gtgcggtgtt gattcccagg gagtccttga
     4981 gggtggagtt ggttctcgcc gcatcgggca acactggaac gtcaatgcgt agaccgaggg
     5041 tcagcgcgag ccgtgcgact ggcgtccaac ggtcttgcgt gtagagaccg acctggcgca
     5101 cgtggaagtc gacaacgcgg cgatcggtgc cgggaaggcc gatctcgtag ctggaggcct
     5161 gggccgagtc gagcttgtcg aggctggaga attcccaggt gccggcggat tcgaggagca
     5221 gcgggtcgcg gaaatggagc agctcgttgt gggtgccgag cgtgatcagg tgcgcccccg
     5281 cggtgatggt cagattgtcg gtgagctcga gcacctcttg cgacacgtca ttggtgggac
     5341 ataccatatt tggcccggcg aagagccgcc cgttatccgc ggacacctga attctgggaa
     5401 agtccgcatt cgggcggcag gggtcccgca ggctcagata gctcacctgc agctcgttgg
     5461 cgtgacggcc ggcgagcacg gtggcccaga ccagccgcgt tgaggactgc gtggaggcgt
     5521 cttcctccgc cacggacgac agccggtaga tccccaattc gcgtggcacg aagccgcgac
     5581 ggctaccgtg cgcgtaatgg tgagacaatt ccacccggct gttggtgccg agctgggcgg
     5641 tgaccttgcc gaacagatcc gcggtgggga tgcgcccttc ggacgggccc agcgtcccgg
     5701 ggtcgagccc gtaggttcgc ctgaggatgc tgtcgaaccg cacggcgctg gcgtagcgga
     5761 tcccgatgcg cgtcgtgtcg gcgccgcccg ccgtgtccgc gatgaggggg ccggggtccg
     5821 gaacaacgcg ttggtgcagg tccgcgctcg cgaagtactg cagccgatcg cgcacgacgg
     5881 gaccggccgc ccacccgccg acttgccaga tcgcaaaatc ctccgcgcct gctcgcgccg
     5941 tagggcgggc cgcgaagctt tggttctcga agaacgtgaa caccgagcca tgcgacgtgt
     6001 tggtgcccga tctcgtcacg acattgacca gcccacccgc aaagccgccc tggcggacgt
     6061 cgaacggcgc caccagcacc tggaattcct ggacggcttc catggaaatc gtgcgcggca
     6121 gcacttgcgg caccgccgca gcgggaatgc ctccgcccgg ctcccggccg aggtacgcct
     6181 cgccgctcgc gccgccgtcg atctgaaagc ggttcaacac cgggttctgc gccccgatgg
     6241 acatagcgcc ccccgaccgg agcgagacgt atgggctgag caacgccagg ttcgcgaggc
     6301 tgtgcgccag attcgggaac cgcgccacgg cagagtctga aatcagctgc gtgggaccgg
     6361 tgtaacccca gttgatgagt ggggcggccg tcgccgcgac tgtgaggggc tcgagctgga
     6421 tcgccgcggg ttggaggacg agatccgcga cgtatcgttg accgagcgcg agggccggga
     6481 tcgccagacg gaccgccgcg aaccccaacg cgcggaccac gatggaatag ggtccaccta
     6541 ccggcacgtt ctcgagcgca aacacgccgc tggacgctgt ggagacccgc cactgctggc
     6601 cgttcgggac gctgatgatc gctacgatcg cgccggcgat cgggcggccg tccggctcga
     6661 cgacgcgtcc ctggatcgca gccgatgtca caccttgggc tcggagtgaa gcagcggcca
     6721 ggagaaggct caagcggtga aatccgcgca gccgcagcac ggcgccgcta ccccttggcc
     6781 ttgagcagcg tcacgaatgc cgagtcctgg cgcaggggag cgagaaacgg gtcgatatcc
     6841 atcggcgggc cgtggggctc gtagcctacc agcgtgcgcc ccagggctcc ctcggtcaga
     6901 gcctgacgca ggaggtcgag cgcctgctgg cgctggccca gctgtactgc gatgcaggca
     6961 cgccagtaag tccgattgac ctggtcgtat ggtccacggt cctcggccag cgcccgttcg
     7021 ctgtgctcgg ccgtgccccg ttcgccgcgc cgggcggcca gcacgccgag gaggccaacg
     7081 taccgctcgt tgccaggatg ctcgcgcgcc agtcgctcgg cgatggggta cgcctcctgc
     7141 actcgcccca tgaaggcgaa cgtctgggcc aggttcaact ggccgatctc agtcgcctgc
     7201 tcctctggtg gccgactgag ctgccagcgg agggcctgca tgaggaggga atcggccatg
     7261 gaggaaccgt gcagctggag ctccgcggcc gtccagagca taacctccgc cggagtcctc
     7321 cccaacgggt gggcagccat ggctgccagc tcctcgagac cctcgttgac ctcccgggtc
     7381 cgaccgagcg ccgccagtgc cgacagctcc cgacggagca tggtgaggtc gttcggatac
     7441 tgctgccggg cacgacgggc ggccgcgagc tcgtgcgcgt agtccccgac gcggtggtag
     7501 gcggtcgtca gccagatata gtacgggcgg tgcccccgca aagcgccgcg gtcgggatgc
     7561 agcccctcca ggattgcgat cgcttcgcgc gggcgattca tcacgagcgc ctgccgggcg
     7621 gcgaccgtcg cccacatctc cgaggcggga gcggcttccc atagttcgtg cgacaggcgg
     7681 tatgcggctt cctgatcacc gcggcagatc gcgacttcga ggtccacgtg cagcaggtcg
     7741 atgcgtcccg catacgggga gcgtgcatgc agtacccggc cgagggagtc ggttatcgcg
     7801 cattcacgga gaaacgcgtg cccccacgcg gcccagacga gcgggagggt gaagctcgag
     7861 tcgagtgcag ccgcgcgata gaagtgctcc aacccctcgc gcgctttcag ttggtcgtac
     7921 cacgcatgca cgcccgccat gaattcacga taggcttcgt aactcggcgg ttggctggcg
     7981 agccctgccc aatcccggag cttggggtct agcgcggtcc ccagtgctcc tgttacccgc
     8041 tgcgccaggg cggcgatcgc gggccgcggg tcagccagac tcgccgtcac gggcccgaca
     8101 ctgcgcaaca gcgtccgacg tcgttcgtcg gtgatctgag cctccagctc cacactgtca
     8161 ccgcggcggt agtaatcgcc ccagacggcc aggcctgaac cgctctcgag tgcgagccga
     8221 cgcgcgccct ctgcggcacc cggagacggg gccgtgggtc caccgccgcc cgcgggtccc
     8281 cgcgccaggc gctcgcttgc gggatcggcg acttccacca ggcccgtctg cgccaggctc
     8341 cgggcgatcc actcggccgc gagttggccc agggccgaca gcgtggtgtc gccggtacgg
     8401 ttgtccaacg gtgcgatcag cacgcggttg gaacggaggt cgggagcggc gtgccggcgt
     8461 gccccggcca ccacgccaac agccgccagg ccgatcgcgg ccgcgacgcc gatgggccag
     8521 gcgaggcggc cggaacgagc tcgggatggg ctcggcgtga gcggtgcggt ggcggtcgcc
     8581 ggggcctgat gtccctgcgg tatcggcggg ccggccggct cgagcggcgc ggatgccgat
     8641 tgccgcccgc gcacggcggc catcagagcc ttcgtttccg gagccggctc cacgccgagg
     8701 tccgcgacga gccgtcgttc gaaggtctcg taaagctgcg ccgcgcccgc gcgatcgccg
     8761 agccgatcca ggagctcgat agtgcgacgc acgaggatct catcatgcgg cgccagttcc
     8821 agcgcacgac gataggtcgt tacggctgag actaggtccc ccgccgcttg cagctcttcg
     8881 gcccggcgtc gggcgccggc ggcggcgact gtgcgcagtc gcacccgctg cttctccatc
     8941 cattcctcga actcgggcgc gtgagcgatg aagaacccta cgagcagatc tccgcggtag
     9001 agctcgagcg cctcgtccaa ccgtccagca gccagcgcgc tctcgaagga gacgacgtcg
     9061 caggagacgc gcccgaagtc gaggccgatc tcgtcgtcgc cgcgactcac cacgacggcg
     9121 cccagggcgc cgcgaatcac gcgcagcgct tggcgcaacg cggcgcgcgc gtgctcctga
     9181 tcgagttccg gccagaacag ggtgaggatc acgtcgcggc gctggaggcc ccgcggtgtc
     9241 gccgccgcga ggtaagccag gagcgcgagc cgtcgcggct gcgcgaggag accgcccgcc
     9301 tcacgaccgt cgccgtccct gagggaaacg ggccccagta tccggaactc gattgcagca
     9361 gccatcgacc tggccttgtg tcgcgggcgc acacggctgt cacggttgcg tcacggagcc
     9421 gtgccagtcc cgcccgatac tccggatgga aacatggacc gggtttggcg gacggcaaat
     9481 catcttggca ggaggctacg atgaacgcaa ggcggaagct cacagcagct gcggcattgt
     9541 ctgcgctggc ggtagcatgc aatgtcgaga cggagtcggt tgggccgacc ctatcggtat
     9601 ttgacctaca atccgaggcg tcctcggcgc cgctcgtcct gcacctcgtc gcgccgcagg
     9661 ccacggaccc ggcgatcgac cagttcctcg ccgatcacta cgtctggctc gacactgcgg
     9721 cacatcccaa ccacaagctc ttcgtgctca tgcccggcac cggtcagatc cctgccgcct
     9781 tccagttcgt agaacgggag gcggcgcggc tcggctacgc cgtgatcggc ctgtcgtatc
     9841 tcaacagcgg cggcttcgcg aaggtgtgcc cgctcgcgac cgacccagcc gcctgctacg
     9901 aggacagccg gctcgaaatc gtcgacggca tcgagcgca
//
LOCUS       DBNGZW010000127         5843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_829622, whole genome shotgun sequence.
ACCESSION   DBNGZW010000127 DBNGZW010000000
VERSION     DBNGZW010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_829622"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..1324)
                     /locus_tag="AC16JC_03150"
     CDS             complement(143..1324)
                     /locus_tag="AC16JC_03150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163723.1"
                     /translation="MAIVVVSSYNVASFPEGGGHFWVYMQYVHGLQQAGCDVYWLERF
                     RRGKDPARDAQAMTTFFDRMQRFALEGKVLLYTQDDGPEGGESAIEFVGVPGGRPAAE
                     AILRRADLLLNFHYAIDPGLLGCARRTALVDIDPGLLQFWMSTGQLHVPPHDAYLTIG
                     ETVGTPAARFPDCGLSWIPFRPPVCLDLWPYTFDPRSNAFTTVAGWWSGQWVRGMVDG
                     HEIFYENTKRVSFLEFVELPGRTRQPLELALYLAKNDGADLALLDRHGWRVRRSQDVA
                     GSPEDYHGYVQRSRGEFSCAKPSCMKLQNAWISDRTLCYLASGKPAVVQHTGPSAYLP
                     DGEGLFRFTTVEEAAAALEAVNADYERQCRAAREIAVTWFDAKQVLSHILNATLPVAA
                     C"
     gene            complement(1336..2007)
                     /locus_tag="AC16JC_03155"
     CDS             complement(1336..2007)
                     /locus_tag="AC16JC_03155"
                     /inference="COORDINATES: protein motif:HMM:NF016668.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HIR5163724.1"
                     /translation="MKLLIVNADDFGLTRGTSRGIVEAHRRGIVTSTSLLVDAPGSAA
                     AAALAEDLPGLSVGLHLDLDGVAPEYVPAELERQLARFGELTGSPPTHLDTHHDSHGD
                     PAILRHVLAFARRHDLLLRGHSTVHVCSRFYGQWGGETHLEQISVAGLARVVSEGAGD
                     GITELICHPGHPDPELRSSYAAEREAEVGTLCDPGARAVLDTQGIRLIGFRDLPALVA
                     GAHRW"
     gene            complement(2004..3092)
                     /locus_tag="AC16JC_03160"
     CDS             complement(2004..3092)
                     /locus_tag="AC16JC_03160"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR5163725.1"
                     /translation="MGVLTTGDLGDVLEAERLPGAAELGAALQEVCSDADGAARILEL
                     RRLKTNVYRVRLEAGDGSARSLMLKSSNPALARHNQLVARRWLPAIGLADAGARLEGS
                     AADRHGQRIWLIYEDLGDARLDTAATDRSRVGAVVDLIAALHVRSAGHAVLPDCRAHG
                     GDLGAPYLCGNVRDAVRGLEQLCTPRVALTPEQESIRDRLLERLRALQHELPDRLLML
                     ELGGGPEVLLHGDLWTTNAFALAGPRPGEWRARLIDWDHAGVGWASYDLSTFLLRFPR
                     EERPWIVERYRAALGNAGAGWRLPGTRELNAVFETAEFARFANRAVWPSVELLVDGAT
                     WAFEELSMVAGWFDEWEPVLAEPGERPR"
     gene            complement(3080..4081)
                     /locus_tag="AC16JC_03165"
     CDS             complement(3080..4081)
                     /locus_tag="AC16JC_03165"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR5163726.1"
                     /translation="MSRAPIAVLHEGLEAHPAVQAWTGMWAAQGMPERVEVLRRLTST
                     EVYRLVGAGPAGKSVIAKRSPGVKASIEQIVYERVLPHLPVTAPHYYGSRPTDGEGRN
                     GGRWIFLEDVGSQRYSEETPTHLALAARWISRMHGAAAGLVAARFLPDGGPGRYLVHL
                     RAARDKIEQRLPGPELTRDDIAMLQGVVAVLNALELRWTRVEAMCLGLPPTVVHGDFR
                     PKNVYLRPNGTGLACYPIDWETAGWGVPAIDLTRIDVAGYWACAREWRPGLELDTVRR
                     LSSIGHVLRTVAAIDWETSTLRYDARRMFSRELASLEVLVRRLAEAAWNSGILEWVC"
     gene            complement(4078..>5843)
                     /locus_tag="AC16JC_03170"
     CDS             complement(4078..>5843)
                     /locus_tag="AC16JC_03170"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5163727.1"
                     /translation="VAGAYSDFTLYRRLLAQARPYWPHLGVLFALGLLTTPIALLVPL
                     PLKIVVDSVLGTRPLPGPLALWFPAAHSPETALAIAIGVLLTVTLLAQLQSLFNNWLR
                     ASVGEHIVLGFRSRLLEQSQRLSLSYHDSTGTADTAYRIQYDASALQYVAVDGLIPFV
                     SATITLVAMLSVTARLDWQLATVALAVAPILFALSRTFRRRMRGQSRAVKKLESSALH
                     VVQEVLGALRVVKAFNQEPRETERFMGRAREGVAARLRLALIEGRFGLWVGLTTASGT
                     AAVLYLGVGHVRAGVLTLGELLMVMSYLAQLYQPLSTISRKAASLQSYLASAERAFAL
                     LDEGPDVPERPDARALVRASGAVEFRDVCFGYDADRAVLRDVSFTVAPGTRIAVTGAT
                     GAGKSTLMSLLTRFYDPTSGAVLLDGVDLRDYRVADLRRQFAIVLQHPLLFSTTIAEN
                     IAYGRPGAPREAIEQAATAANAHDFISRLPRGYDTQVGELGQKMSGGERQRIALARAF
                     LRDAPVLILDEPTSSVDLKTEALILDALERLMTSRTVFLITHRPSTWTMCDVRLQIER
                     GRLVRREDPAVAAMAGKGEER"
BASE COUNT          885 a         2092 c         2062 g          804 t
ORIGIN      
        1 ggatcgtcga gccggccgtc cagggtaggg gctcggtcga tccgcgccgc cgataccacc
       61 tggggggccg cgccgccggt gtgacgcagc gacgtcgagt cgggcagggc gccctgcagc
      121 gcgacggccg cggcggcgag gatcagcacg cggccaccgg cagcgtcgcg ttgaggatgt
      181 gcgagagcac ctgtttcgcg tcgaaccagg tgacggcgat ctcccgggcc gcccggcact
      241 gccgctcgta gtcggcgttc acggcctcca gtgcggccgc cgcctcctcc accgtggtga
      301 accggaacag gccttcgccg tcggggaggt aggcgctcgg gccggtgtgc tgcaccacgg
      361 ccggtttgcc gctcgccagg tagcacagcg tgcggtccga gatccaggcg ttctggagct
      421 tcatgcagga cggtttggcg cagctgaact ccccgcgcga ccgctggacg tagccgtgat
      481 agtcttcagg gctccccgcg acgtcctgag agcgccgcac ccgccagccg tgtcgatcga
      541 gcagggccag gtcggcgccg tcgttcttgg cgagatacag ggccagctcg agcggctggc
      601 gcgtgcgccc cggcagctcc acgaattcga ggaacgacac gcgtttggtg ttctcgtaga
      661 agatctcgtg cccgtccacc atcccccgta cccactgtcc ggaccaccag cccgccaccg
      721 tggtgaacgc gttcgatcgc gggtcgaacg tgtacggcca gaggtccagg cagaccggcg
      781 gccggaaggg gatccaggag agaccgcaat cggggaagcg cgccgcgggc gttcccaccg
      841 tctccccgat cgtcaggtag gcgtcgtgcg gcggcacgtg cagctggccg gtgctcatcc
      901 agaactgcag cagccccgga tcgatgtcga cgagcgccgt gcgccgcgcg cagccgagca
      961 gtccggggtc gatggcgtaa tggaagttca gcagcaggtc ggcccgccgg aggatcgctt
     1021 cggcagcggg cctccctccg gggacgccca cgaactcgat tgcggactcg ccgccctccg
     1081 ggccgtcgtc ctgcgtgtac agcagcacct tgccctcgag cgcgaaccgc tgcatccggt
     1141 cgaagaacgt cgtcatcgcc tgcgcgtccc gcgcggggtc cttgccgcgc cggaaccgct
     1201 ccagccagta gacgtcacac ccggcctgct gcagaccgtg gacgtactgc atgtacaccc
     1261 agaaatgccc gcccccttcg gggaagctcg cgacgttgta cgaggacacg acgacgatgg
     1321 ccacgatgct ctcctctacc agcggtgcgc gccggccacg agggccggca ggtcgcgaaa
     1381 gccgatcagg cggatgccct gcgtatcgag gacggcgcgc gcgccggggt cgcacagggt
     1441 gccgacctcg gcctcgcgct cggcggcgta gctcgatcgc agctcggggt ccgggtgtcc
     1501 gggatggcag atcagctcgg tgatgccgtc gcccgcgccc tccgacacca cgcgcgcgag
     1561 cccggcgaca ctgatctgct ccaggtgggt ctcgccgccc cactggccgt agaaccgcga
     1621 gcacacgtgg accgtggaat gcccgcgcaa cagcaggtcg tgccgccggg cgaacgccag
     1681 gacgtggcgc aggatggcgg gatcgccgtg actgtcgtga tgggtgtcga gatgcgtcgg
     1741 gggggagccc gtgagctccc cgaaccgcgc cagctggcgc tcgagctccg ccggcacgta
     1801 ctcgggcgcg acgccgtcca ggtcgagatg caaccccacg ctcaggccgg gaaggtcctc
     1861 cgcgagcgcc gccgccgcgg cgctccccgg cgcgtccacc agcaggctcg tgctggtcac
     1921 gatgccgcgc cggtgcgcct ccacgatgcc ccggctggtg ccgcgcgtga ggccgaagtc
     1981 gtccgcattg acgatcagca gcttcatcgc ggccgctccc cgggctccgc cagcaccggc
     2041 tcccactcgt cgaaccaccc cgcaaccatc gacagctcct cgaacgccca cgtggcgccg
     2101 tccacgagca gctcgacgga gggccacacc gcgcgattcg cgaagcgggc gaattcggcc
     2161 gtctcgaaca cagcgttgag ctcccgtgtc ccgggcagcc gccatcccgc accggcgttc
     2221 cccagcgcgg cgcggtaccg ctcgacgatc catggccgct cttcccgcgg gaaacggagc
     2281 aggaacgtgg acaggtcgta gcttgcccac cccacgccgg cgtgatccca gtcgatgagc
     2341 cgggcgcgcc attcgccggg gcgggggccg gccagcgcga acgcgttggt cgtccagaga
     2401 tcgccgtgca gcagcacctc gggcccgccg cccagctcca gcatcaggag ccggtcgggc
     2461 agctcgtgct gcagcgcgcg aagacgctcc agcaggcgat cgcgtatcga ttcctgctcg
     2521 ggcgtcaacg cgacccgtgg cgtgcacagc tgctcgagac cgcgcaccgc gtctcgcaca
     2581 ttgccgcaca ggtagggcgc tccgaggtcg ccgccgtgcg cccggcagtc cggcagcacc
     2641 gcgtgtccgg cggaccggac atgcagcgcg gcgatcaggt ccaccaccgc gccgacgcgc
     2701 gagcggtccg tcgccgccgt gtcgagtcgt gcgtcgccca ggtcttcgta gatcagccag
     2761 attcgctggc cgtggcggtc ggccgcggag ccctccaacc gggcgcccgc gtccgccagg
     2821 ccgatcgccg gcaaccagcg gcgggccacg agctgattgt gacgcgccag cgcggggttg
     2881 gaggacttga gcatgagcga gcgggcgctc ccgtcccctg cctcgagccg cacgcgatag
     2941 acgttggtct tgagccgacg cagctcgagg atacgggcgg caccatcggc gtcgctgcag
     3001 acctcctgca acgccgcgcc gagctccgcc gccccgggga ggcgctccgc ctcgagcacg
     3061 tcgccaaggt cgccggtcgt cagcacaccc attccaggat tcccgagttc catgcggctt
     3121 cggccaggcg gcgcaccagc acctccaggc tcgcgagctc ccggctgaac atccggcgcg
     3181 cgtcgtaccg gagggtggag gtctcccagt cgatggcggc cacggtgcgg agcacgtgcc
     3241 cgatgctcga cagccgccgc accgtgtcga gctccagccc gggccgccac tcgcgcgcgc
     3301 acgcccaata gccggcgacg tcgatccggg tcaggtcgat ggcgggcacg ccccaaccgg
     3361 cggtctccca gtcgatcggg tagcaggcga ggccggttcc gttggggcgg aggtacacgt
     3421 tcttcggacg gaagtcgccg tgcaccacgg tcgggggcag ccccaggcac atggcttcga
     3481 cgcgggtcca acgcaattcc agggcgttca ggaccgcgac cactccctgc agcatggcga
     3541 tgtcgtcgcg cgtcagctcg gggccgggca gccgctgctc gatcttgtcc cgggccgcgc
     3601 gcaggtgtac gaggtagcgc cccggaccgc cgtccggcag gaagcgcgcg gcgacgaggc
     3661 cggccgccgc cccgtgcatc cgcgaaatcc atcgggcggc cagcgccaga tgggtcggcg
     3721 tctcctcgga gtagcgttgg gagccgacgt cctcgaggaa aatccagcga ccgccattgc
     3781 gcccttcgcc gtccgtgggc cggctgccgt agtagtgggg cgccgtgacg ggcaggtgcg
     3841 ggagcactcg ctcatacacg atctgctcga tggacgcctt cacgcccgga gagcgcttgg
     3901 cgatcacgct cttgccggcg gggccggcgc ccaccagccg gtacacttcc gtgctcgtga
     3961 gtcggcgcag cacctcgacg cgttcgggca tcccctgtgc ggcccacatg cccgtccatg
     4021 cctggaccgc cggatgggcc tccaggcctt cgtggagcac cgcaatcggc gcgcggctca
     4081 tcgttcttcc ccctttccgg ccatcgccgc gacggcggga tcttccctgc ggaccaaccg
     4141 cccgcgctcg atctgcagcc gcacgtcgca catcgtccag gtgctcggcc ggtgagtgat
     4201 caggaaaacc gtgcggctcg tcatcaggcg ctccagtgca tcgaggatca gtgcttcggt
     4261 cttgaggtcc accgagctgg tcggctcgtc gaggatcaac acgggcgcgt cgcgcaggaa
     4321 ggcgcgagcg agcgcaatcc gctggcgctc acctccggac attttctgcc ccagctcacc
     4381 gacttgggtg tcgtagccgc gcggcaaccg ggagatgaag tcgtgggcgt tggcggccgt
     4441 cgccgcctgc tcgatggcct cgcgcggggc gccgggccgg ccgtaggcga tgttctcggc
     4501 gatggtggtc gagaagagca ggggatgctg cagcacgatg gcgaactggc gccgcaggtc
     4561 ggcgacgcgg taatcgcgca ggtccacccc gtcgagcaac accgcaccgc tcgtggggtc
     4621 gtagaagcgg gtgagcaggc tcatcagcgt ggacttaccc gctccggtcg ctcccgtgac
     4681 cgcgatgcgg gtgcccggcg cgaccgtgaa cgagacgtcg cgcagcaccg cccggtcggc
     4741 gtcatacccg aagcagacgt cccggaattc gacggcgccg ctcgcgcgaa cgagcgcgcg
     4801 cgcatcgggg cgctccggga cgtccggccc ttcgtccagc agggcgaagg cgcgctcggc
     4861 gctcgccagg taggactgca ggctggccgc cttgcgactg atagtggaca gcggctggta
     4921 gagctgggcc aggtagctca tgaccatcag cagctcaccc agggtcagca cgccggcccg
     4981 cacgtggccg acccccaggt agagcacggc cgccgtgccg ctcgcggtgg tcaacccgac
     5041 ccacaacccg aagcgtccct cgatgagcgc cagccgcaga cgggccgcga cgccctcgcg
     5101 ggcgcgaccc atgaaacgct cggtctcgcg cggctcctgg ttgaacgcct tgacgacgcg
     5161 cagcgccccg aggacttcct gcacgacgtg cagggctgag ctctcgagct tcttgacggc
     5221 ccgggattgc ccccgcatgc ggcgccggaa agtccgcgag agcgcgaaca ggatgggcgc
     5281 gaccgccagc gcgaccgtcg cgagctgcca gtcgaggcgc gcggtcaccg agagcatcgc
     5341 gaccagcgtg atcgtcgccg agacgaacgg gatgagaccg tccacggcga cgtactgcag
     5401 cgccgaggcg tcgtactgga tccggtaggc agtgtcggcg gtgccggtcg agtcgtgata
     5461 ggagagcgac agccgctggc tctgctccag caggcgtgac cggaatccca gcacgatgtg
     5521 ctcgcccacg gaggcgcgca gccagttatt gaacagactc tgcagctggg ccagcagcgt
     5581 gacggtcagc agcacgccga tggcgatcgc cagtgccgtc tccggcgagt gcgcggccgg
     5641 gaaccacagg gccagggggc cgggcagggg ccgcgtgccg aggaccgagt ccaccacgat
     5701 cttcagtgga agcggcacga gcagggcgat gggggtggtg agaagcccga gcgcgaacag
     5761 caccccgaga tgcggccagt aggggcgcgc ctgggcgagc agccgccggt acagggtgaa
     5821 gtcgctgtag gctcccgcca ccg
//
LOCUS       DBNGZW010000128         3800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_353555, whole genome shotgun sequence.
ACCESSION   DBNGZW010000128 DBNGZW010000000
VERSION     DBNGZW010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3800
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_353555"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2893
                     /locus_tag="AC16JC_03175"
     CDS             <1..2893
                     /locus_tag="AC16JC_03175"
                     /inference="COORDINATES: protein motif:HMM:NF020698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
                     /protein_id="HIR5163728.1"
                     /translation="STACHASGGGGGGGGGVSATNSTVTASPTSFTAGTGSSTITVTA
                     KDGNGATISGASVTLTASGSGNTFTPASGTTNGSGVLTSTFTSSVAEVKTISATINGT
                     AITQTAAVTVNSSGGGGGGGNGTISHTLLTAAGSGANQSVYTTASIAPAANALVTVAV
                     RMRRSTGPLTPAISGGGMTSWTLVMTLDYGTIATPDSRLSVFRAMSASPGSGPISITF
                     SGGISNADWSVSQWSGVDQSGTNGSGAIGQVGSTAGDATTSLSKTLAAFASPNNVALG
                     VVAAKLAPPAVTPGSGFTEIAEATSGETTVLESEWGANLNTVSATLSGSTAAGLLAIE
                     LKAGSSGPTVSAANSTVSASTPITAGGAGSTITVTAKDGSGNPISGATVVLAATGSGN
                     TLTQPAGTTNASGVATGTLASTVAEVKTVSATINGTAITQQATVTVNPAAAAALAFTT
                     QPSNTQANASIAPAVVVDVRDQFGNHVNSSASVAMAIGTNPGSGTLAGTTPKGAINGV
                     ATFDDLAINNTGTGYTLVASSSGLTSATSAAFNITAPAPSATLSTVVAGTPSFTAGGS
                     SAITVTVKDGLGDPLSGISVTLSSSVVGDVVTQPAATTDGSGVASGSVAATSAGSRTV
                     TAVAGGITLTQKPSLTVTAAGPNAGTSTVSASPTSIIAGSGASTITVTVRDQYGNPVS
                     GSSVVLASTGTNVTVTQPSGSSGSNGVATGSVSSADAQTLSVSATAGAVAITQTASVT
                     VTNQPPPGITQTLLTSGHDPNNVSTFTTASIAPAANALVTVAVLTHQASSAAPSPTLS
                     GGGMTSWTVVASIAYNGATPLDRLTIYRAMSASPGSGPITIASSLTVSNCQWIVSQWT
                     GVDQSGTNGSGAIVQTASASGSAVTSLTAALAAFASPNNVAYGAFGAASATSVITAGS
                     GFTTIDQQPSGESTIGDLFAEWAVNLPAVTASWSSRNAGALGVEIKAAP"
     gene            complement(2897..3271)
                     /locus_tag="AC16JC_03180"
     CDS             complement(2897..3271)
                     /locus_tag="AC16JC_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR5163729.1"
                     /translation="MKTVATDHAPKAIGPYSQALVADGVVYTAGQVALDPQTGTLVGT
                     TIAEQTEQVLKNLSAVLAAAGSSLGQVVKTTVYLADMADFAAMNEVYARHFGGHRPAR
                     STVQAGGLPKGARLEIDAIAIQ"
     gene            complement(3280..>3800)
                     /locus_tag="AC16JC_03185"
     CDS             complement(3280..>3800)
                     /locus_tag="AC16JC_03185"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF013436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR5163730.1"
                     /translation="ADEEIEAVRAACAAEGVDSVRGDVWEKGGEGGREVAQAVLDLLD
                     QGKAAFKPLYDERRPIAEKIETIAKEIYGAGNVAYAPAAARALELLPRLGLGETPVCM
                     AKTQYSFSDDPTKLGAPAGYTLQVRDILPSAGAGFVVALAGDIMTMPGLSKSPAAERI
                     RLHPDGTIEGLF"
BASE COUNT          601 a         1409 c         1262 g          528 t
ORIGIN      
        1 gagcaccgcc tgtcatgcct ccggcggcgg tggcggcggt ggtggtggcg tctcggcgac
       61 gaactctacg gtcacggcct cgccgacctc gttcacggcc ggcaccggat cctcgacgat
      121 taccgtcacc gccaaggacg gcaacggtgc tacgatcagc ggcgccagcg taacgctgac
      181 cgcctcgggg agcggtaaca ccttcacccc ggcttcgggc accaccaacg gcagcggcgt
      241 gctcaccagt acgttcacct cctccgtggc cgaggtgaag accatttcgg ccaccatcaa
      301 cggcactgca atcacccaga ccgccgcggt gacggtgaat tcgtcgggtg gcggcggtgg
      361 aggcggcaac ggcaccatca gccacacgct cctgaccgcg gccggcagcg gcgccaatca
      421 gagcgtgtac accaccgcgt ccatcgcgcc ggcggccaac gccctggtga cggtcgccgt
      481 ccgcatgcgc cgcagcaccg gtcccctgac gccggcgatc tccggcggcg ggatgacgag
      541 ctggaccctg gtgatgacgc tcgattacgg aacgatcgcc acgcccgatt cgcgcctcag
      601 tgtgttccgg gcgatgagcg ccagccccgg cagcgggccg atcagcatca cattctccgg
      661 cggcatctcc aacgcggact ggagtgtgtc acagtggagc ggtgtcgatc agagcggcac
      721 gaacgggtcg ggggccatcg gtcaggtcgg ctcgacggcc ggcgatgcca cgacgtcgct
      781 ctccaagacg ctcgcggcct tcgccagtcc caacaacgtc gctctggggg tcgtggcggc
      841 gaagctcgct ccgcccgcgg tgacgccggg cagcggcttc accgaaatcg ccgaagcgac
      901 ctccggcgag accaccgtgc tcgagagcga atggggcgcc aacctcaaca cagtgtcggc
      961 caccctgagc ggctccaccg ccgccggtct gctggcgatc gagctgaagg cgggtagcag
     1021 tgggcccacg gtgtccgcag cgaactccac cgtctcggct tcgactccga tcacggccgg
     1081 cggtgccgga tccaccatca ctgtgaccgc gaaggacggc agcggcaatc cgatcagcgg
     1141 cgcgaccgtc gtgctggcgg ccaccggcag cggcaacacg ctcacccagc ccgcgggcac
     1201 cacgaacgcg agcggcgtgg cgacgggcac gctcgcctcg acggtcgccg aggtgaagac
     1261 cgtctcggcc accatcaacg gcacggcgat cacccagcag gcaaccgtca ccgtcaaccc
     1321 cgcggcggcc gcggccctcg ccttcaccac gcagccgagc aacacgcagg cgaacgcgtc
     1381 gatcgcgccg gccgtcgtgg tcgatgtccg cgaccagttc ggcaaccacg tcaactcctc
     1441 ggccagcgtc gcgatggcaa tcgggacgaa tccgggatcg gggacgctgg cgggcacgac
     1501 ccccaaggga gcgatcaacg gcgtcgcgac cttcgacgac ctggcgatca acaacaccgg
     1561 cacgggctac acgctcgtcg ccagctcgtc cggtctcacc agcgccacct cggccgcctt
     1621 caacatcacg gcgcccgccc cctcggccac gctctcgacg gtcgtcgccg ggactccgag
     1681 cttcacggcg ggcggcagct cggccatcac cgtgaccgtg aaggacggcc tcggggaccc
     1741 tctgagcggc atctccgtca cgctgtcgtc gtcggtggtg ggcgacgtcg tcacccagcc
     1801 ggccgccacg acggacggca gcggagtggc ctcgggaagc gtcgccgcca ccagcgccgg
     1861 ctcgaggacc gtcacggcgg tcgcgggcgg gatcacgctc acgcagaaac cctcgctgac
     1921 cgtcaccgcc gccggtccga acgccgggac ctcgaccgtg agcgccagcc ccacgagcat
     1981 catcgcgggc agcggcgcct ccacgatcac cgtgaccgtg cgcgatcagt acggtaaccc
     2041 ggtgagcggc tcgagcgtcg ttctggcgtc gaccggcacc aacgtcaccg tcacgcagcc
     2101 cagcggcagc agcggctcga acggggtggc cacgggaagc gtgagctccg ccgacgctca
     2161 gacgctctcc gtctccgcca cggccggcgc tgtggcgatc acccagaccg cctccgtgac
     2221 ggtgaccaat cagccgccgc ccggcatcac ccagacgctg ctgacctcgg gccacgaccc
     2281 gaacaacgtc tcgaccttca ccacggcgtc gatcgcaccc gccgcgaacg cgctcgtgac
     2341 ggtggcggtg ttgacccacc aggcgagctc ggcggcaccg agccccacgc tctcgggtgg
     2401 cggcatgacg agctggaccg tcgtggcgag catcgcctac aacggcgcca ccccgctcga
     2461 tcgcctgacc atctaccggg cgatgagcgc gtcgccgggc agcgggccga tcacgatcgc
     2521 ctcgagcctc acagtctcca actgccagtg gatcgtgtcg cagtggaccg gggtggatca
     2581 gagcggcacg aacggatcgg gcgccatcgt gcagacggcg tcggcgagcg gctctgccgt
     2641 gaccagtctg acggccgcgc tggcggcgtt cgcgagcccc aacaacgtgg cctacggggc
     2701 gttcggcgcc gccagcgcga cgtcggtcat cacggccggc agcggattca ccacgatcga
     2761 ccagcagccc tccggcgaga gcacgatagg cgatctcttc gcggagtggg cggtgaatct
     2821 gcccgcggtc accgcgagct ggtccagcag gaacgccggg gcgctggggg tggagatcaa
     2881 agcagcgccc tgagcgctac tgtatcgcga tcgcgtcgat ctccaggcgg gcgcccttgg
     2941 gcaatccacc ggcctggacg gtggatcggg ccggccgatg gccgccgaaa tgccgggcgt
     3001 agacctcgtt catcgccgcg aaatccgcca tgtcggcgag gtacacggtg gtcttcacga
     3061 cctgccccag ggaagacccc gccgccgcga gcacggcgga caggttcttg aggacctgct
     3121 cggtctgctc ggcgatggtg gtgccgacga gtgtccccgt ttgcgggtcg agcgccacct
     3181 gccccgccgt gtatacgacg ccgtcggcca cgagggcctg gctgtagggg ccgatcgcct
     3241 tcggcgcgtg gtcggtcgcg acggtcttga tggacttcat cagaacaggc cctcgatggt
     3301 gccgtcggga tggagccgga tccgctcggc agcgggactc ttgctcaacc ccggcatggt
     3361 catgatgtcg cccgccagcg ccacgacgaa tcccgcgccg gcggacggga ggatatcgcg
     3421 cacctgcagc gtgtagcccg ccggcgcgcc gagcttcgtc gggtcgtcgc tgaaggagta
     3481 ctgcgtcttc gccatgcaca ccggcgtctc tcccagtccc agcctgggga gcagctcgag
     3541 cgcgcgcgcc gccgccggcg cgtaggcgac gttccccgca ccgtagatct ccttcgcgat
     3601 ggtctcgatc ttctcggcga tggggcggcg ctcgtcgtac agcggcttga acgcggcctt
     3661 cccctgatcc agcagatcga ggaccgcctg cgccacttcc cggccgcctt cccctccctt
     3721 ttcccagaca tcgccgcgga ccgagtcgac gccctccgcc gcgcacgccg cccgcaccgc
     3781 ctcgatttcc tcgtcggcgt
//
LOCUS       DBNGZW010000129         4516 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1238577, whole genome shotgun sequence.
ACCESSION   DBNGZW010000129 DBNGZW010000000
VERSION     DBNGZW010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4516)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4516)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4516
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1238577"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="AC16JC_03190"
     CDS             complement(<1..621)
                     /locus_tag="AC16JC_03190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163731.1"
                     /translation="MNGREFWMRWRSDILRGVVIFAVVLGGGLFVRSLVHRGRAQLNG
                     LERAFDSDFFAADRQRAEPWTYVTPLASQRTLWLRNINGSINIEPAAAGSVEIRAERT
                     FRHSAADSVRIVTAESEKGITVCAVWPSANGAGACGPDGHYTSEPRLRGNDVAVVFTV
                     RLPRGVKVDASTINGDVEVRGASAPIGVGTVNGDVTVETSGGPVRAV"
     gene            complement(709..975)
                     /locus_tag="AC16JC_03195"
     CDS             complement(709..975)
                     /locus_tag="AC16JC_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163732.1"
                     /translation="MRILALSLGAVGSLGVVALGGRTIAADRTDHARRAPIAAPAIAA
                     PAIATSSAADRVWYGGTLAPIVVVADPPEHARAHAARDCPAPGS"
     gene            1098..1868
                     /locus_tag="AC16JC_03200"
     CDS             1098..1868
                     /locus_tag="AC16JC_03200"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5163733.1"
                     /translation="MPTLSRFRDLLIQLILRDLKSRYKQTFFGALWVFGRPLVELGAY
                     VLVFGAFLRAPSDGVPYPLFAFSGVVLWSFVSGAVSRGARAVQSERGLVAHAPFPKAT
                     LPLAAIAGALIDSALAAVMLGLLMVMQRVTPSPEAWLLLPVLLIVTALVTGLSLIGSA
                     LNVFYRDVGHLVDMGMRVWLLLTPVAYASSVVPSRFKALYDLNPLVSVFNFSRAALLG
                     RGDLDPLSLWYPTTVGVVLLAIGAWVFRVTEPYFAESV"
     gene            1872..3086
                     /locus_tag="AC16JC_03205"
     CDS             1872..3086
                     /locus_tag="AC16JC_03205"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5163734.1"
                     /translation="MAITVSGVWKRYRLRSRSLKELLSNVLPRRSTARPPRPKTMWAL
                     EDVSFEVKPSSTFALVGPNGSGKSTMLKLIAGMMNPTRGSIHITGRLSLLSFLGGSFH
                     GDLTGRENVFLEGALLGLSRGELKAKLDAIFAFAELERFIDVPIKFYSAGMALRLGFA
                     IASHLEPEILLIDEAFAVGDTAFRDRCLGRMLAFKAAGTTMVLVSHERYLVEQLCDTA
                     LLLDHGRVVAHGRPEEAFAAYERVIEAEEGSDADEREGEPARSPLDIESVEILGGEGA
                     TDPLFHSDAGLTLRIHLQARRDVAGASVGVQIAREWHVLHGTRCNRQGIDITARAGER
                     VTLELQYGSLTLARGSYALNVLIYEHRLAVEPVVVWKRAARFRVALPEKEGVGLIRLA
                     HEWRLAPDGRSR"
     gene            complement(3088..4089)
                     /locus_tag="AC16JC_03210"
     CDS             complement(3088..4089)
                     /locus_tag="AC16JC_03210"
                     /inference="COORDINATES: protein motif:HMM:NF026257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5163735.1"
                     /translation="MTTGGLLFDTLRFHGAPPPERLAAAWAGADVRGLSRLVALEGCA
                     IWAYRRLRQVGALDRMDPDLADWLAAQMTDQTARNLLIDSEADALAEVFRRIDVPAVF
                     MKGVARRLLADRFPLADARGTHDVDLIVPAERAWEVWHELRRLGYERTRLTKPPRPEH
                     HHLPALMCERRVGVEIHTTHAQGIPAAESWRRLHTGAAEVVRGGRRFSVPSATELFWN
                     GTAHALRVPDVAFVLGHLLDTAVIWASDTPLDWPEIVRRLDAKEIVDTVAAGSWLRAA
                     ADLAGVMPPPELEPRLGACDLTRALDLRLVVLRRVPVPAAWNKLLIWWSSERARRGS"
     gene            complement(4086..>4516)
                     /locus_tag="AC16JC_03215"
     CDS             complement(4086..>4516)
                     /locus_tag="AC16JC_03215"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HIR5163736.1"
                     /translation="GGLPLGWRLRAAAAVLIVPPLLHFISFARLASWLGGSSSGKVEP
                     GLDDAALAHWVDRLLRNLPGPWHRTCLKRSAVLYHLLRRAGRPVELCIGVRNEEPVSV
                     AAHAWLVRGGAPYLEARPEHAGRHTVIARFPEKRAAAARS"
BASE COUNT          671 a         1622 c         1526 g          697 t
ORIGIN      
        1 caccgcgcgc accggtcctc cggaagtctc gacggtgacg tcaccgttga ccgtgcccac
       61 cccgatgggt gccgaggcgc cgcgcacctc gacatcgccg ttgatcgtcg acgcgtccac
      121 cttcaccccg cggggcaacc ggacggtgaa gacgaccgcg acatcgttgc cccgcagcct
      181 gggttcggac gtgtagtggc cgtcggggcc gcaggctccg gcgccgttgg cgctgggcca
      241 cacggcgcac acggtgattc ccttctcgga ttcggccgtc acgatccgga ccgaatcggc
      301 tgcggaatgc cggaaggtgc gctccgccct gatctccaca ctgcccgccg cggccggctc
      361 gatgttgatc gagccgttga tgttccgcag ccacagggtg cgctgcgacg ccagcggcgt
      421 cacataggtc cacggctcgg cgcgctggcg atccgcagcg aagaagtcgg aatcgaacgc
      481 gcgctccaga ccgttcagct gggcgcgacc ccggtgcacc agcgagcgca cgaacagccc
      541 gccacccagg accaccgcga agatcacgac gccgcgcagg atgtcgctgc gccaccgcat
      601 ccagaattcc cggccgttca tacgaatccc cccgtggggt gtggctgaaa gaccgccggc
      661 cctacggggg gaaggtacaa ccggtttcgc cgagatgccg ccggccggtc agcttccggg
      721 agccgggcaa tcgcgtgcgg cgtgcgcccg tgcgtgctcg ggcgggtccg ccacgacgac
      781 gatgggggcg agcgtgccgc cataccacac gcgatcagcc gccgatgacg tggctatcgc
      841 cggcgccgcg atggccggcg ctgcgatggg cgcgcgtcgc gcatggtccg tccggtccgc
      901 cgcaatggtg cggcctccca atgccaccac gcctagactg ccgacggccc cgagcgataa
      961 tgccaggata cgcatacacc ctccttaact cctgagacgc cacgtccgac ggcgtggtca
     1021 cctgattgtg acgcacaaac accacagaaa gttgcagcgc tcgtgtccgg gccccgtcat
     1081 gcattagttt tgcatccgtg ccgaccctgt cgcgcttccg tgacctcctc attcagctca
     1141 tcctgcggga cctcaagtcg cgttacaagc agacgttctt cggcgctctg tgggtcttcg
     1201 gccgtcccct ggtggagctg ggcgcgtacg tcctggtgtt cggcgcgttc cttcgtgcac
     1261 cgtcggacgg cgtcccgtat ccgctcttcg ccttctccgg cgtggtgctg tggagcttcg
     1321 tctccggggc cgtttcccgc ggggcgcgcg cggtgcagag tgaaagaggc ctggtggccc
     1381 acgcaccgtt cccgaaagcg acgctccccc tcgccgccat cgcgggcgcc ctgatcgact
     1441 cggccctggc ggccgtcatg ctcggcctgc tgatggtgat gcaacgcgta acgccctccc
     1501 ccgaggcctg gctgctgtta ccggtgctgc tcatcgtcac ggcgctcgtg acgggcctgt
     1561 cgctgatcgg gtcagcgctg aacgtgttct accgcgacgt ggggcatctg gtggacatgg
     1621 ggatgcgcgt gtggctgttg ctgaccccgg tcgcctatgc ctcctcggtc gtcccgtccc
     1681 gcttcaaggc gttgtacgat ctcaacccgc tggtgtcggt gttcaacttc tcccgcgccg
     1741 ccctgctggg ccgcggcgat ctcgatcccc tgtcgctctg gtacccgacg acggtgggtg
     1801 tcgtcctcct cgcgatcggc gcgtgggtgt tccgtgtgac cgaaccgtac ttcgccgaat
     1861 cggtgtagcc cgtggccatc accgtctcgg gggtctggaa gcgctatcgg ttgcggagcc
     1921 ggtcgctgaa ggagctgttg tcgaacgtgc tcccgcggcg gagcacggca cggccgccgc
     1981 gccccaagac catgtgggcg ctcgaggacg tgtcgttcga ggtcaagccc agctccacct
     2041 tcgcgctcgt cggccccaac gggtcgggca agtccacgat gctgaagctg atcgcgggca
     2101 tgatgaaccc cacccggggc tcgatccata tcaccgggcg gctgtcgctg ctcagttttc
     2161 tcgggggatc cttccacggc gatctcaccg ggcgcgagaa cgtgttcctg gagggggcgc
     2221 tgctcgggct gtcccgcggc gagctgaagg ccaaactcga cgcgatcttc gccttcgccg
     2281 agctggagcg cttcatcgac gtgccgatca agttctattc ggccggcatg gccttgcggc
     2341 tggggttcgc catcgcctcg cacctcgagc ccgaaatcct gctcatcgac gaggccttcg
     2401 cggtgggcga caccgccttt cgcgaccgct gcctcgggcg gatgctcgcc ttcaaagccg
     2461 ccggtacgac catggtcctc gtctcgcacg agcggtacct ggtggagcag ctgtgtgaca
     2521 ccgcgctgct gctggatcac ggacgcgtcg tcgcacacgg tcgaccggaa gaggcgttcg
     2581 ccgcctatga acgggtgatc gaggcggaag agggaagcga cgccgacgaa cgggagggcg
     2641 agccggcccg ctcgcccctc gacatcgagt cggtggagat cctgggcggc gagggagcca
     2701 ccgatcccct gttccacagc gatgccggcc tcacgttgcg catccacttg caggcccgcc
     2761 gcgacgtcgc cggcgcctcc gtcggggtcc agatcgcccg ggagtggcac gtgctgcacg
     2821 ggacgcgctg caaccgtcag gggatcgaca tcacggcgcg cgccggcgaa cgagtgaccc
     2881 tcgagctcca gtacggttcc ctgacgctcg cgcgcggctc ctacgcgctc aacgtgttga
     2941 tctacgaaca ccgcctcgcg gtggagccgg tggtggtctg gaaacgcgcc gcccgattcc
     3001 gcgtcgcgct gccggagaag gagggggtgg gactgatccg gctcgcccac gagtggcgtc
     3061 ttgctcccga cggccggagc cgctaggtca ggaacctcgg cgcgcgcgct cgctgctcca
     3121 ccagatcagc agcttattcc acgccgccgg gacgggcacg cgccgcagga cgaccagccg
     3181 cagatcgagc gcccgcgtca gatcacaggc gccgagccgt ggctcgagct ccggcggcgg
     3241 catgacgccg gcgaggtcgg cggccgcccg gagccaggac cccgccgcca ccgtgtccac
     3301 gatttccttc gcatccagcc ggcgaacgat ctcgggccag tccagcggcg tgtcgctcgc
     3361 ccagatgacg gcggtgtcga gcaggtgccc caggacgaac gcgacgtccg gaacgcgcag
     3421 ggcatgggcg gtgccgttcc agaacagctc agtcgccgat gggacgctga agcgccggcc
     3481 gccccgcacg acctcggcgg cgcccgtgtg cagccgacgc cacgactccg cggctgggat
     3541 gccctgagcg tgcgtcgtgt gaatctcgac gccgacgcgc cgctcgcaca tcaacgcggg
     3601 caggtgatgg tgctcgggcc ggggcggctt ggtcaaccgg gtgcgctcgt agccgagacg
     3661 acgcagctcg tgccacacct cccacgcccg ctcggccggg acgatgaggt cgacgtcgtg
     3721 cgtgccgcgg gcgtcggcaa gcggaaaacg gtccgccagg agcctccggg cgacgccctt
     3781 catgaagacc gcgggcacgt cgatccgccg gaagacctcg gccaacgcgt ccgcctccga
     3841 gtcgatcagc aggttccggg cggtctggtc ggtcatctgc gcggcgagcc aatcggcgag
     3901 gtcgggatcc atccggtcca gcgcgccgac ctggcgcagc cggcggtagg cccagatcgc
     3961 acacccttcg agtgccacga gccgcgagag tccacggacg tcggcgccgg cccatgccgc
     4021 cgccagacgc tcgggcggcg gcgcaccgtg aaaccggagc gtatcgaaca gcagaccacc
     4081 ggtggtcacg aacgcgccgc ggccgctcgt ttctccggaa accgtgcgat caccgtgtgt
     4141 cggccggcgt gctcgggccg agcctcgagg tagggagcgc ccccgcgcac cagccaggca
     4201 tgcgccgcca ccgacaccgg ctcctcgttg cgcacgccga tgcacagctc cacggggcgc
     4261 ccggcccgcc gcagcaggtg atacaacacc gccgatcgct tgaggcaggt gcggtgccac
     4321 gggccgggaa gattgcggag caaccgatcc acccagtgcg ccagcgccgc gtcgtcgagg
     4381 ccgggctcga ccttcccgga ggagctcccg cccaaccagc tcgccagccg cgcgaacgag
     4441 atgaaatgca agagcggcgg cacgatcagc accgccgccg ccgcccgcag acgccagccc
     4501 agcggaaggc ctccac
//
LOCUS       DBNGZW010000130         4144 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_283967, whole genome shotgun sequence.
ACCESSION   DBNGZW010000130 DBNGZW010000000
VERSION     DBNGZW010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4144)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4144)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4144
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_283967"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..948)
                     /locus_tag="AC16JC_03220"
     CDS             complement(235..948)
                     /locus_tag="AC16JC_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163737.1"
                     /translation="MKLTGVVVAVLAGVPLPLMAQRSGDRAGGGLREVRDIGNAGVNL
                     VVAVPVGEFRQNVDVAGGLDVFGAFNLGRSGLALRLDGSYLIYGSDNTLVDQPYYPVS
                     INTTYSITSLGIGPQLTFGQGPVRLYGFGTVGFSYIWARSSYGVGGCGCDAFASGTDF
                     DDWTGALQGGGGLLLNLRSRHVPIALDIGARYLSNGDAWYVSPGDVVPQPNGDVVIYP
                     VRSKANLVMVHAGVSIGLR"
     gene            1087..1422
                     /locus_tag="AC16JC_03225"
     CDS             1087..1422
                     /locus_tag="AC16JC_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017655233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HIR5163738.1"
                     /translation="MAQITYDDFAKVDIRVGRIVSVDDFPQARKPAWRLRIDFGPLGE
                     KNSSAQITRYYDKDDLVGKLVLAVVNFPPRQIANFFSEVLTLGIELGPGDIVLVHPDR
                     DVPLGLPLR"
     gene            complement(1415..1963)
                     /gene="msrA"
                     /locus_tag="AC16JC_03230"
     CDS             complement(1415..1963)
                     /gene="msrA"
                     /locus_tag="AC16JC_03230"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR5163739.1"
                     /translation="MTTDRSESATLGGGCFWCLEAVFLDLKGVERVVSGYAGGSIPNP
                     SYEDVCTGRTGHAEVVQVTFDPAVLSYRDLLQVFFSIHDPTTLNRQGPDVGTQYRSAI
                     FYHGPEQERVAREVIADLERQGVWDSPIVTEVTPAPPFYPAEQYHQEYFRRNPGQGYC
                     RVVIAPKVAKFRKEHLARLKAT"
     gene            2035..3489
                     /locus_tag="AC16JC_03235"
     CDS             2035..3489
                     /locus_tag="AC16JC_03235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR5163740.1"
                     /translation="MLLLRFYDDGLAQASYLIGCQTTGEALIVDPNRDVEQYIAAAEA
                     EGLRIAHVTETHIHADFVSGSRELAQRTGARLYLSDEGGPDWRYGFASQAGAALLKDG
                     TRFQVGTVAIEALHTPGHTPEHLVFLVTDTPAAAAPLGVLSGDFVFVGDVGRPDLLER
                     AVNIRGTMEAGARQLFRSLQRFKQFPDYLQIWPGHGAGSACGKALGAMPQSTVGYERL
                     ASWAFAITEEEAFVTAVLEGQPEPPKYFAEMKRINKEGPRLLGGAGLGRPRRLPAARV
                     GALLAQGALVVDTRAAADFAAAHIPGTISIPLNRAFTGWAGWLIPYDRDFYLLIDHAR
                     EDAVETAARDLAMIGLDRLGGYLDCDDEMLETWRASGGTLESTPLLTPAQLHARLPGG
                     DVTVIDVRGRNEWEVGHLPGVANIPLGLLPDRLTEVPQQGMIVLQCESGSRSAIAASL
                     LQAQGRRAVANLEGGFNAWRAAGYPVVRESPVPA"
     gene            complement(3486..>4144)
                     /locus_tag="AC16JC_03240"
     CDS             complement(3486..>4144)
                     /locus_tag="AC16JC_03240"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR5163741.1"
                     /translation="LPHDHIILSAKVSGVPDLVAVYRKLAARCDYPLHLGLTEAGMGA
                     KGIVASSAALAILLQDGIGDTIRVSLTPRPGGDRTEEVHVAQQILQSLGLRSFTPQVT
                     ACPGCGRTTSTFFQEMAEQIQEYLRRQRPEWQRRYAGSEELRVAVMGCVVNGPGESKH
                     ADIGISLPGTFEEPKAPVYVDGQLRVTLKGDTIVPDFLKILEEYVDRRYGKPAPVGRA
                     "
BASE COUNT          676 a         1387 c         1448 g          633 t
ORIGIN      
        1 gcgcggccga cagcatgctg gccagcaccg ggcggcggat ggcggtttcg gagagtctca
       61 tagtgaccgg gaaagttact cggcgaaaac ggcgatggta ctgtttgtgt caggaaacgg
      121 tggggccggt ctgtcgctca gcaaacgggg ccaggaggcg ctacctcctg accccggtgt
      181 ctgacgacca ttgtgcggct gaacgcctgt ttgaacgttc ggtgactccc cgtactacct
      241 gaggccgatc gatacgcccg cgtggaccat caccagattc gccttgctgc ggacgggata
      301 gatcaccacg tccccgttgg gttgggggac cacgtctcca ggcgaaacgt accaggcgtc
      361 cccgttggac aggtagcgag ctccgatgtc gagcgcgatg ggcacgtggc ggctgcggag
      421 attgagcagc agcccgccgc cgccctgcaa ggctccggtc cagtcgtcga agtcggtgcc
      481 cgaggcgaag gcatcgcagc cgcatccgcc cacgccgtag gacgaccggg cccagatata
      541 ggagaagccg accgtgccga acccgtacag ccggaccggc ccctggccga acgtcagctg
      601 cgggccgatg ccgaggcttg tgatcgagta ggtcgtgttg atggacacgg gatagtacgg
      661 ttggtcgacg agagtgttgt cgctgccgta gatgaggtac gacccgtcga gtcgcagggc
      721 gagcccgctg cgccccagat tgaacgcccc gaagacgtcg agcccgcccg cgacgtccac
      781 gttctggcgg aactccccga ccggtacggc gacgaccagg ttgacgccgg cgttcccgat
      841 gtcgcggacc tcacgcagcc cgccgccggc cctgtccccg gagcgctgtg ccataagagg
      901 aaggggaacg cccgccagca ccgccacaac cacaccggtc agtttcatcg tcgcaacctg
      961 tgggagtcgc cggcgggaag ggcaagcgat atgccaatcg ctggaaggct agatttggca
     1021 ggtccttccc ggccggtgac ctccccggag gacagcgcgc ttcaaccgac acccggagga
     1081 cttcaggtgg ctcagatcac ctacgacgat ttcgccaagg tggacatccg ggtcggccgg
     1141 atcgtgtcgg tggacgactt tccccaggcg cgcaaacccg cctggaggct gcgcatcgac
     1201 ttcgggccgc tgggagagaa gaactccagc gcgcagatca ccaggtacta cgacaaggac
     1261 gacctggtgg ggaagctcgt cctggcggtg gtgaacttcc cgccgcggca gatcgccaat
     1321 ttcttttcgg aggtgctgac gctgggaatc gagctcgggc ccggggacat cgtcctcgtg
     1381 caccccgacc gcgacgtccc gctgggactg cccctcaggt agccttgagc cgggcgagat
     1441 gctccttgcg aaacttggcc accttgggag cgatcaccac gcggcagtac ccctgccccg
     1501 ggttgcggcg gaagtactcc tggtggtact gctccgccgg atagaacggc ggggctggag
     1561 tcacctcggt gacgatgggt gaatcccaca ccccctggcg ctcgaggtcg gcgatgacct
     1621 cacgggcgac gcgctcctgc tcgggcccgt ggtagaagat cgccgagcgg tattgcgtgc
     1681 cgacatcggg cccctggcga ttgagcgtcg tcgggtcgtg gatactgaag aagacctgga
     1741 gcaggtcccg gtaggagagc accgccgggt cgaaggtgac ctgcacgacc tcggcatggc
     1801 cggtgcggcc ggtgcagacg tcctcatagg aaggattggg aatcgagccg ccggcatagc
     1861 ccgacacgac ccgctccaca cccttcaggt ccaggaacac cgcctccaga caccagaagc
     1921 agcctccgcc cagcgtcgcc gattcgctcc gatcggtggt catacgcctc gaatgaacgc
     1981 gtgcgagcgg cggcgtgttc cgcgaaacgg ctcgcctagc tagcatacag ccccatgttg
     2041 ctgctgcgat tctacgatga cggcctcgca caggccagct atctcatcgg atgccagacg
     2101 accggtgagg cgctcatcgt ggatcccaac cgcgacgtcg agcagtacat cgcggcggcc
     2161 gaggcggagg gtctgcgcat cgcgcacgtc accgagaccc acatccacgc cgacttcgtc
     2221 tcggggagcc gcgagctggc gcagcgcacc ggggcacggc tgtacctctc cgacgagggg
     2281 ggccccgact ggcgctacgg gttcgcgagc caggccggag cggcgttgct gaaggacggc
     2341 acgcgcttcc aggtgggcac cgtcgcgatc gaggcgctgc acacccccgg ccacacgccg
     2401 gagcatctcg tctttctcgt gaccgacacc ccggccgccg cggctccgct cggcgtgctg
     2461 agcggcgact tcgtgttcgt gggcgacgtg ggccgtcccg atctgctcga gcgcgcggtg
     2521 aacatccggg gaacgatgga agcgggagcg cgccagctgt tccgctcgct gcagcgtttc
     2581 aagcagtttc ccgactacct gcagatctgg cccggacacg gggcgggttc ggcgtgcggc
     2641 aaggcgctgg gcgcgatgcc tcagagcacg gtgggctacg agcgactggc cagctgggcg
     2701 ttcgcgatca ccgaggaaga agcgttcgtc acggcggtgc tggaaggcca gccggaaccc
     2761 ccgaagtact tcgccgagat gaaacgcatc aacaaggaag gaccgcgcct gctggggggg
     2821 gccggtctgg ggaggccgcg acgtctgccg gcggcgcgcg tgggcgccct gctcgcgcag
     2881 ggcgcgctgg tggtggacac ccgggcggcg gccgacttcg ccgccgcgca cattcccggc
     2941 acgatcagca tcccgctcaa tcgcgcgttc acgggctggg ccggctggct catcccctac
     3001 gaccgcgatt tctatctgct catcgaccac gcccgcgagg acgcggtgga gacggcggcg
     3061 cgcgatctcg cgatgatcgg gctcgaccgt ctgggcggat acctcgactg cgacgacgag
     3121 atgctggaaa cgtggcgggc ctcgggcggg acgctcgaat ccacgcccct gctcacgccg
     3181 gcccagctgc acgcgcgcct ccccggcggg gacgtcaccg tgatcgacgt gcgggggcgc
     3241 aacgagtggg aggtcgggca cctgccggga gtcgcgaaca tccccctcgg cctgttgccc
     3301 gaccggctga ccgaggtgcc ccaacaggga atgatcgtgc tgcagtgcga gagcggatcc
     3361 cgctccgcga tcgccgcgag tctgcttcag gcgcagggcc gccgcgcggt cgccaacctc
     3421 gagggcgggt tcaacgcctg gcgagcggcg gggtatcccg tcgtgcggga gtcgccggtc
     3481 ccggcttagg cccgccccac cggcgccggt ttgccgtagc gccggtcgac gtactcctcg
     3541 aggatcttca ggaaatcggg gacgatcgtg tcgcccttga gcgtgacccg cagctgcccg
     3601 tccacataca cgggggcctt gggctcctcg aacgtgcccg ggagcgagat cccgatgtcg
     3661 gcatgcttgg actcgccggg gccgttcacc acgcatccca tcacggccac gcgcagctcc
     3721 tccgagccgg cgtagcgccg ctgccactcg gggcgctggc ggcgcaggta ctcctggatc
     3781 tgctccgcca tttcctggaa gaaggtggac gtggtgcgcc cgcaccccgg gcacgccgtg
     3841 acctgcgggg tgaagctgcg cagcccgagc gactggagga tctgctgggc gacgtgcacc
     3901 tcctcggtgc gatcgccgcc gggccgcggg gtgagcgaga cgcggatcgt gtcgccgatg
     3961 ccgtcctgca ggaggatcgc cagcgccgcg ctgctcgcga cgatcccctt ggcgcccatt
     4021 cccgcctcgg tgagaccgag atgcagcggg tagtcgcagc gggcggcgag cttgcggtac
     4081 accgcgacga ggtccggaac gcccgagacc ttggccgaga ggatgatgtg gtcgtgcggc
     4141 aggc
//
LOCUS       DBNGZW010000131         1664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1172274, whole genome shotgun sequence.
ACCESSION   DBNGZW010000131 DBNGZW010000000
VERSION     DBNGZW010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1664
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1172274"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..673)
                     /locus_tag="AC16JC_03245"
     CDS             complement(<1..673)
                     /locus_tag="AC16JC_03245"
                     /inference="COORDINATES: protein motif:HMM:NF015427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HIR5163742.1"
                     /translation="MEARLRQALARAEEVAHLLADPETARHPAKLKQLGREHARLQAI
                     RQAHDRLTRLQAELVQAREVLADQDPELSQLARHDIERLGPEIGRLDEQLADLLVPPD
                     PLNDRDAIVEIRAGTGGDEAALFAADLFRMYTRFCERRGWTVELLSLSEGVLGGLKEA
                     VFAARGPRAYCTLRYESGVHRVQRVPATEAQGRIHTSAATVAVLPEAEEVDVKIDDKD
                     LRVDVF"
     gene            complement(679..891)
                     /gene="rpmE"
                     /locus_tag="AC16JC_03250"
     CDS             complement(679..891)
                     /gene="rpmE"
                     /locus_tag="AC16JC_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012532141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR5163743.1"
                     /translation="MKPGIHPRYRRATVQCACGNTFVTRSTIAEIHVEICAQCHPYFT
                     GKQKLVDTAGRVERFRQKYRTEKAAS"
     gene            1283..>1664
                     /locus_tag="AC16JC_03255"
     CDS             1283..>1664
                     /locus_tag="AC16JC_03255"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR5163744.1"
                     /translation="MSVEATLQKVPLFSQLSPADLQRVLEVARERAYPKNSVILFEDD
                     PGDSLYVVKQGQVKVVLIGEDGREVILSVLGEGDFFGEMALIDDEPRSAHVIAMGDST
                     LLVLRREDFQGLLMQTPGIALAMLR"
BASE COUNT          253 a          575 c          558 g          278 t
ORIGIN      
        1 ggaacacgtc gacgcgcaga tccttgtcgt cgatcttcac gtccacttcc tccgcttcgg
       61 gcagcacggc gaccgtggcg gcggaggtat ggatgcggcc ctgtgcctcc gtggccggga
      121 cgcgctgcac gcgatgcacg cccgattcgt agcgcaacgt gcagtaggcg cgcgggccgc
      181 gggccgcgaa cacggcttcc ttgagcccgc ccagcacgcc ctcgctcagg ctgagcagct
      241 cgaccgtcca gccgcggcgc tcgcaaaagc gggtgtacat gcggaacagg tcggcggcga
      301 acagcgccgc ttcgtctccg ccggtgcccg cacggatctc cacgatcgcg tcgcggtcgt
      361 tgagcggatc gggggggacg agcagatcgg cgagctgctc gtccagccgg ccgatctcgg
      421 ggcccagccg ctcgatgtcg tggcgggcca actgcgacag ctccgggtcc tggtcggcga
      481 ggacttcacg agcctgcacc agctcggcct ggagacgcgt cagtcgatcg tgagcctgcc
      541 ggatggcctg cagacgggcg tgctcacgac cgagctgttt cagcttggcc ggatgccgcg
      601 cggtctcggg gtcggccagc aggtgggcga cctcctccgc ccgcgcgagc gcctggcgga
      661 gtcgggcctc catcgtgttc agctcgcagc cttctccgtg cggtatttct ggcggaagcg
      721 ctccacccgc cccgcggtat ccacgagctt ctgcttgccc gtgaagtagg ggtggcattg
      781 cgcacagatt tccacgtgga tttccgcgat cgtcgaccgc gtcacgaagg tattcccgca
      841 ggcgcactgc acggtggcgc ggcggtaccg gggatggatt ccaggtttca cgacgtcatt
      901 cctctgtgtc acagcgtatg aatcaccggc gcaccgggcg ccgcgcgaag catggaaata
      961 tagtcgccgc agcaacttgg gggcaaggcg ggggtcgcgt tcccacgcgt tcttgcattg
     1021 gccggacttg cggcccacat cgtcccgcgc ctccccgcgt cgcacccggc caaccccatt
     1081 gaggagtgaa tcgcggactc atctactgcg tccgagccgc tgcgccccag cccaccggcg
     1141 cgccggatgg cccgcagccc cgccggaact gcaatcccct cagcgagttg aatccaccac
     1201 cccagtgccg gggcgaattg acaccgaagc caccgcgcca taagcttgcc ccatcccaac
     1261 gccccctccg gggagttcgc gcgtgtccgt cgaagcgacg ctgcagaagg tgccgttgtt
     1321 cagccagctc agccccgccg acctgcagcg ggtgctcgaa gtcgcgcgcg agcgtgccta
     1381 ccccaagaac tccgtcatcc tgttcgaaga cgaccccggc gattcgctgt acgtggtcaa
     1441 gcaggggcaa gtcaaggtgg tgctgatcgg cgaggacggc cgcgaagtca tcctgtcggt
     1501 gctgggcgag ggcgacttct tcggcgagat ggctttgatc gacgacgagc cgcgctccgc
     1561 gcacgtcatc gcgatgggcg actcgacgct gctggtcctg cgacgtgagg atttccaggg
     1621 gttgctgatg cagaccccgg ggatcgcgct cgcgatgctg cgcg
//
LOCUS       DBNGZW010000132         6993 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_417502, whole genome shotgun sequence.
ACCESSION   DBNGZW010000132 DBNGZW010000000
VERSION     DBNGZW010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6993)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6993)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6993
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_417502"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(159..1295)
                     /locus_tag="AC16JC_03260"
     CDS             complement(159..1295)
                     /locus_tag="AC16JC_03260"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012692896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR5163745.1"
                     /translation="MVPAVAPVIARQRAAALVALGLLATALPGVAAAQGGPKLKVPPG
                     FAIDVFADKVGSVRFMAVDPAGTLLVSEPSAGRVLALPDKNGDGKADGVQTVVTGLDQ
                     PHGLAFRDGALYVAETSRVQRFAYDPAGMKATQPTLLARLPSGGGHWTRTVVFGPDGL
                     MYVSVGSSCNVCRESDKRRAAVLRFNADGSGEQVFASGIRNAVGLTFHPGTGALWATV
                     NERDWRGDDVPPDYVTEVREGTFHGWPDCMVVRGRVTADTAFAKGAACDKVTPPTVEI
                     QAHSAPIGLAFYTGTQFPEDYRGTLFVAYRGSWNRTLPTGYKIVRIPFRDGQAAGAPE
                     DFATGWLEGPSSWGRPVDLVVGRDGALYLSDQGAGRIYRITYRP"
     gene            complement(1271..2143)
                     /locus_tag="AC16JC_03265"
     CDS             complement(1271..2143)
                     /locus_tag="AC16JC_03265"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR5163746.1"
                     /translation="MRLPPDRFDYSPLPTREPWKLPRGARIAVWTIVNVEEWDIEKAM
                     ARQYLPTPQGVAVVPDVPNWAWHDYGMRVGFWRLLDALTKRKIRATTAINAHVVESYE
                     PVARAMLDAGWEFMAHGFVQGAMHLLPDQRAAIRQCVELLEKFTGRKPKGWLGPGLTE
                     TWETLDVLKAEGIEYVSDWVNDDQPYEIRTRSGPLVSVPYSIELNDIPMMVIQHHTSG
                     EWLQRAKDQFDRLYTEGAKNPRVMALAVHPYISGVAHRIKYFEAVYDYMRKKPGVWMT
                     TGEEIYEWYRQSRR"
     gene            2366..4948
                     /locus_tag="AC16JC_03270"
     CDS             2366..4948
                     /locus_tag="AC16JC_03270"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR5163747.1"
                     /translation="MRSGIRLILFATTAAAAVFGGPLSTEAATVHHVNRSDAGCGGHA
                     PCYASIQAAVSAAQAGDTVQIQPGTYVEQVSIVGKNAATWSEASRILIQADPAAPVGS
                     VILRGAVAQCEQGPAIRIQQSRFVTIRGLTITGAGGAGIALLGGGSQNVSIRIERNRI
                     AGNGGPECDGGITIAGGNAGTLILNNVIVANGRNGIATLDADGGPHTIVQNTIHGNGW
                     NGVSATRAHVLLLVNNAITGNGVQAGSTGGRAGVRRETATGLTATTIVLRSNLVCGNR
                     LGELAGPLLDGLDSGNLTPTGAEGPGVTASPGCAVPAVVYRDLAGADHGLGTLDDDPT
                     PAPGSPLIDRGLDPRTLLTPDLNPRFEADYFTEAVRPAAGAVGGAPRFDIGAVEARRD
                     AEPPAVAFLTPGVNAHLRGVAPVQARATDSGGGVASLSIRVGSQALTATLDPSPPAAA
                     ITATASWSTSALADGVQTLTVVATDQGQNTATATRTVIVDNTPPETQITGGPDGTSSS
                     PAATFTFAGSDNLTPASSLAFAWRMDDGAFSPFSPTASAMLSGLTPGAHTFEVKARDL
                     AGNEDPQPARRTFSVASGDVVVAITEPSAGATVPSGVALVRGTVANGGAGAGVAVNGV
                     VASVQGGVFAATVPVSAPSITITAVATTETGSSATDSVTVSVPDVGESAVSLRASPRM
                     GGTPLVTSFSLVGGPVPARVELDVDGDGSIDFSGPSLEGQTFTYPAAGLYFPVVRVID
                     GQGGASTARAVVHVLDRTDLETLLQPKWTALRDALGRGDVPGAVALFAGASREAYQQQ
                     LTALAGAGALPQVAADLGPITAVRVLDRAAEYELRAVQQGVTYSFYVLFVVDTDGVWR
                     LRVF"
     gene            4969..5538
                     /locus_tag="AC16JC_03275"
     CDS             4969..5538
                     /locus_tag="AC16JC_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163748.1"
                     /translation="MLLAAVGEGRAAAEDEEGTLRAPAGPYRGRVVDRQTGQPVPDAL
                     VVILWQRFDEIEGLRRLVAARETFTDSNGEFVHDVGALESKLPPRTLTPRLLVFRPGF
                     TSAPSRPQVSPPGVPARQFTGPGGVATVTAVSNYEDRAEAFNTFIAMLSNAQLFPPTG
                     LPGTEELIRFELQSLGVRPPTPVAPRGGR"
     gene            6131..>6993
                     /locus_tag="AC16JC_03280"
     CDS             6131..>6993
                     /locus_tag="AC16JC_03280"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase C/P1 nuclease family protein"
                     /protein_id="HIR5163749.1"
                     /translation="MMVSIAIAMVLLCLLPFDAEPYEIGTHEELTRASSKRSSIDEVL
                     KDDLGLVGGVRTAVRGRLLEDWLVQGAAREDAFPRFLNHFHNPLASDWAQAGLGSTVG
                     QSAIRWAQNAAQAVPSWSWSDTRRAYFEALTLESNTDRDGRLGATFEGIGRQTHLVQD
                     AASPGHARNDPHLLYNYETLIDDVLERQTSVFQGWMDVGADGVPDADWRSLDSNPLAP
                     IAIARLIDTDRYSGSNPAVTTEALIGLAEYTNANFFSEDRVFTESDLNPQARFPYPKR
                     SSVVEQAFDVTA"
BASE COUNT         1066 a         2417 c         2455 g         1055 t
ORIGIN      
        1 tgagcgcggc gatcgactgg aactggaagc ccatcgacgt gcgcgtgagg aagatgacca
       61 gcagcatggc ccagcggagg tttgctccgc gcgcggccag accacgggcg tgagggggcg
      121 gcgacagatc gatgcgggtc atcgatgggt tcgctcgctc agggccggta ggtgatgcga
      181 tagatgcggc ccgcgccctg gtccgacaga tagagggcgc cgtcgcgccc caccaccagg
      241 tcgaccggcc ggccccacga cgagggaccc tcgagccagc cggtggcgaa gtcctccggc
      301 gcgcccgcgg cctgcccgtc gcggaagggg atgcgcacga tcttgtagcc ggtgggcagc
      361 gtgcggttcc acgagccgcg gtaggccacg aagagggtgc cccggtagtc ctccggaaac
      421 tgcgtcccgg tgtagaacgc gagcccgatc ggcgcggagt gcgcctggat ctccacggtc
      481 ggcggcgtca ccttgtcgca cgccgcgccc ttcgcgaagg cggtgtccgc cgtcacgcgg
      541 ccgcgcacca ccatgcagtc gggccagccg tggaaggtgc cctcgcgcac ctcggtcacg
      601 tagtcgggcg gcacgtcgtc gccgcgccag tcgcgctcgt tcacggtcgc ccagagcgcg
      661 ccggtgcccg ggtggaaggt caggcccacc gcgttgcgta tcccggaggc gaagacctgc
      721 tcgcccgagc cgtccgcgtt gaaccgcagc accgccgcgc gccgcttgtc gctctcgcgg
      781 cagacattgc aggacgagcc caccgagacg tacatgagcc cgtcgggccc gaagaccacg
      841 gtgcgcgtcc agtgcccgcc gcccgagggc aggcgcgcca gcagggtggg ctgcgtggcc
      901 ttcatgccgg cgggatcgta ggcgaagcgc tgcacgcgag aggtctccgc gacgtagagc
      961 gcgccgtcgc ggaaggccag gccgtgcggc tggtcgagcc cggtcaccac cgtctgcacg
     1021 ccgtcggcct tgccgtcgcc gttcttgtcg ggcagggcca gcacgcggcc ggcggagggc
     1081 tcggacacca gcagcgtgcc cgcggggtcg acggccatga agcgcacgct gcccaccttg
     1141 tcggcgaaca cgtcgatggc gaagccgggt ggcaccttga gcttgggccc gccctgggcc
     1201 gcggccacgc cgggcagggc ggtggccagg aggcccaggg ccacgagcgc ggcggcccgc
     1261 tgccgtgcga tcaccggcgc gactgccggt accactcgta gatctcctcg ccggtggtca
     1321 tccacacgcc gggcttcttg cgcatgtagt cgtacaccgc ctcgaagtac ttgatacggt
     1381 gcgcgacgcc cgagatgtac gggtgcacgg ccagcgccat cacccgcggg ttcttcgccc
     1441 cctcggtgta gagccggtcg aactggtcct tcgcgcgctg cagccactcg ccggaggtgt
     1501 ggtgctggat caccatcatc gggatgtcgt tcagctcgat cgagtagggc accgagacga
     1561 gcggccccga ccgggtgcgg atctcgtagg gctgatcgtc gttgacccag tcggagacgt
     1621 actcgatgcc ctccgccttc aggacgtcga gcgtctccca cgtctcggtg aggccggggc
     1681 ccagccagcc cttgggcttc cggccggtga acttttccag cagctcgacg cactggcgga
     1741 tggcggcccg ctggtcgggc agaagatgca tcgcgccctg gacgaaccca tgagccatga
     1801 actcccagcc ggcgtccagc atcgcgcggg ccaccggctc gtaggactcg accacgtggg
     1861 cgttgatggc ggtggtggcg cggatcttcc gcttcgtcaa cgcgtccagc aggcgccaga
     1921 agccgacgcg catcccgtag tcgtgccagg cccagttggg cacgtcgggc acgaccgcca
     1981 ccccctgggg cgtgggcagg tactggcgcg ccatcgcctt ctcgatgtcc cactcctcga
     2041 cgttgacgat ggtccacacc gcgatgcggg ccccccgggg cagcttccag ggctcgcggg
     2101 tgggcaaggg cgagtagtcg aagcgatcgg ggggtagtct cacggggcct cctccctcgc
     2161 ggagtgtact cgactatacg ggaagcgcca ggggggagcc aggacggccg atgcggcctg
     2221 ccggtgccct ccggccgtgg cagtgatcga ggcggagtgc accgtcggcg gtgagacctc
     2281 cgccgggtcc agcgagcaag tccgcgccgg gccgggcccc gcgcattccg cgcaccgtgg
     2341 cacgagcgct gctctcgatc gggacatgcg ctccgggatc cggctgatcc tgttcgcgac
     2401 gaccgcggcg gctgccgtct tcggggggcc actctccacc gaggccgcca cggtccacca
     2461 cgtgaaccga tcggacgccg ggtgcggcgg acacgcgccg tgctacgcga gcatccaggc
     2521 cgcggtgagc gcggcccagg ccggcgacac cgtgcagatc cagcccggca cctatgtcga
     2581 gcaggtgagc atcgtgggca agaacgcggc gacatggagc gaggcgagcc ggatcctcat
     2641 ccaggccgac ccggccgcgc cggtcggcag cgtgatcctg cgcggggcgg tcgcgcaatg
     2701 cgagcagggg cccgcgatcc ggatccagca gtcgcgcttc gtgacgatcc gcgggctcac
     2761 catcaccggc gccggcggcg ccggcatcgc gctgctcggg ggcggcagcc agaacgtctc
     2821 cattcgcatc gagcgcaacc gcatcgcggg caacggcggg cccgagtgcg acggcgggat
     2881 caccatcgcc ggcggcaatg cgggcaccct catcctcaac aacgtcatcg tcgccaacgg
     2941 gcgcaacggg atcgccacgc tcgacgccga cggcggcccg cacacgatcg tgcagaacac
     3001 gatccacggc aacggctgga acggcgtgag cgcgacgcgc gcgcacgtgc tgctgctggt
     3061 gaacaacgcc atcaccggca acggcgtgca ggccggctcc acggggggac gcgccggggt
     3121 gcgacgggag acggccaccg ggctcaccgc gacgacgatc gtgctgcgga gcaatctcgt
     3181 ctgcggcaat cgcctgggcg agcttgcggg cccgctgctc gacggtctcg acagcggcaa
     3241 cctcaccccg accggtgccg agggccccgg ggtgaccgcc agcccggggt gtgcggtgcc
     3301 tgcggtcgtc taccgggatc tcgccggcgc cgaccacggg ctcggcaccc tcgacgacga
     3361 tccgacgccg gccccgggct ctccgctcat cgaccgtggc ctggacccgc gcaccctcct
     3421 gacgcccgat ctgaacccgc gcttcgaggc cgactacttc acggaagcgg tgcgccccgc
     3481 ggccggggcc gtgggcgggg cgccgcggtt cgacatcggc gcggtcgagg cgcgccgaga
     3541 cgcagagccg ccggccgttg ccttcctgac cccaggcgtg aatgcccatc tgcgcggcgt
     3601 cgcgcccgtc caggcgcggg cgacggatag cggagggggc gtggccagtc tcagcatccg
     3661 ggtgggcagc caggcattga ccgcgactct cgatccctcg ccacccgccg cggccatcac
     3721 cgccaccgcg agctggagca cgagtgcgct cgccgacgga gtacagacct tgaccgtcgt
     3781 ggcgaccgat caaggccaga acacggcgac cgctacccgg acggtcatcg tggacaacac
     3841 gcccccggag acgcagatca cgggcgggcc cgatgggacg agctcgagcc ccgcggccac
     3901 cttcacgttc gccgggtccg acaacctgac gccggcgtcg agcctcgcgt ttgcctggcg
     3961 gatggatgac ggagcgttct cgcccttttc tccgaccgcc tccgcgatgc tcagcggcct
     4021 cacgccgggg gcccacacct tcgaggtgaa ggcccgggac ctggccggca acgaggatcc
     4081 gcagccggcc cgccggacct tctcggtggc gtccggagac gtggtggtcg ccatcaccga
     4141 gccgagcgcc ggcgccaccg ttccttcggg ggtcgcgctc gtccgtggta cggtcgcgaa
     4201 tggtggggcc ggagccggcg tcgccgtcaa cggcgtggtt gcgagcgtgc aggggggcgt
     4261 cttcgcggct acggtaccgg tgtcggcgcc gtcgatcacg atcaccgcag tcgccacgac
     4321 cgagaccggg agctccgcca ccgacagcgt gaccgtgtcc gtccccgatg tcggcgagag
     4381 cgcggtgtcg ctgcgcgcga gtcctcggat gggaggaacg cccctggtga cgtccttctc
     4441 gctggtcggc gggccggtac cggcccgtgt cgagctggat gtcgacggtg acgggagcat
     4501 cgacttcagc ggaccctcgc tcgaggggca gacgttcacg tacccggccg ccgggctcta
     4561 cttccccgtg gttcgcgtga tcgatggcca gggcggcgcc tccaccgcgc gggccgtggt
     4621 acacgtgctc gatcgcaccg acctcgagac cctcctccag ccgaagtgga cggctctccg
     4681 cgacgccctc ggccgcggcg acgtgccggg cgcggttgcg ctattcgcag gggcgtcgcg
     4741 cgaggcctac cagcagcagc tcaccgccct cgccggtgcg ggtgcgctgc cgcaggtggc
     4801 ggccgatctc ggcccgatca cggcggtgcg cgtgctcgac cgggccgcgg agtacgagct
     4861 gcgggcggtc cagcagggcg tcacatattc cttctacgtt ctgttcgtgg tcgacacgga
     4921 cggggtatgg cgcctgcggg tgttctgagg ctcgcggcgg cactcgcaat gctgctcgcc
     4981 gcggtcggcg agggccgcgc ggccgcggaa gacgaggagg ggactctgcg cgcgccggcc
     5041 gggccctatc ggggccgcgt ggtggatcgg cagaccggcc agcccgtccc ggacgctctc
     5101 gtggtcatcc tgtggcagcg cttcgacgag atcgagggtc ttcgccgtct ggtcgccgcg
     5161 cgcgagacct tcaccgacag caacggagag ttcgtgcacg acgtcggagc cctggaaagc
     5221 aagctcccac cgcgcactct caccccccgg ctcctcgtct tccggcccgg gttcacctcg
     5281 gcgccgagtc gcccccaggt gtcgccgccc ggcgtgccgg cccgccagtt cacggggccc
     5341 ggcggggtgg cgacggtcac cgccgtatcg aactacgagg accgcgccga ggcgttcaac
     5401 acgttcatcg cgatgctgag caacgcccag ctctttccgc ccaccgggct ccccggcacc
     5461 gaggagctga tccgcttcga gttgcagagc ctgggggttc gacctcccac gcccgtggcc
     5521 ccacgaggtg gccgatgaac ggttgggttc agatggtggc ggggtgctct tggctgctgc
     5581 tggtgtgcgc cgcctcggcg gccgctcaga ggcctgaagg gtgggatcgg tatctggacc
     5641 gaccgcgcgg gccgtatcgg gggcaggtgc tcgacgcgga gaccaaggcg ccgcttacgg
     5701 gcgcggtcgt cgtggcgctg tggcgacggg atcgcgtgta ccccttccac atcacgaccg
     5761 aacactatgc ggtgagggaa acggtcaccg actctgaagg gcgcttcgta ttggaggtaa
     5821 aggacattga agagggagcg ccacgccgga cccggcgacc agtgttcttg atcttcctgc
     5881 ctggctatgg atcgtttcca cgaatgcaga ggtcccctac gggattccta ggtgatgttt
     5941 ttgagcggga caggaccgct gtcgagctgc cccgcttgga gggtcgggag gaccgtctcc
     6001 gaaacctaga caccgttagt ccgcatagtt tgtcggagag cccgcaccaa gaccttccac
     6061 ggctcttgcg agcagtggat gaagaacgct ccgcggtagg gctcaagcca tacccctcgc
     6121 cggagaagcg atgatggtct cgattgcaat cgccatggtt cttctttgtc tcttgccgtt
     6181 cgatgctgag ccgtatgaga tcggaaccca cgaggagctg actcgggcat ctagtaagcg
     6241 ttcaagcatc gatgaagttc tcaaagacga tcttggactt gtcggaggag tacgtacggc
     6301 cgtccgtggg aggcttctgg aagactggct cgttcagggg gcagcgcgag aggacgcctt
     6361 tcctcggttc ttgaaccatt ttcacaaccc ccttgcctcc gattgggcgc aggctgggct
     6421 gggtagcacc gtaggacagt cggcgatccg ttgggctcag aacgcggccc aggcggtccc
     6481 gagttggtca tggagcgata caagacgagc gtacttcgaa gccctcacct tagaaagcaa
     6541 caccgatcgg gacggaagac tcggcgcgac gttcgaaggt attggacggc agactcacct
     6601 cgttcaggac gccgcctcac caggtcatgc tcgaaacgac ccccacctcc tatacaacta
     6661 cgaaaccctc atcgatgacg tgctcgaacg tcagaccagt gtctttcaag gatggatgga
     6721 tgttggtgcg gatggcgtgc cggacgccga ctggcggtcg cttgacagca atccgctagc
     6781 ccccattgcg atcgcgcgtc tcatcgacac cgatcgctac tccggcagca acccagcagt
     6841 aactaccgaa gcgctgatcg gcctggcgga gtacacgaac gccaacttct tcagtgagga
     6901 tcgtgtcttc acggagagcg acctcaaccc gcaggcgcgc ttcccgtacc cgaagcgctc
     6961 cagcgtggtc gagcaggcct tcgacgtgac cgc
//
LOCUS       DBNGZW010000133         3944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1307680, whole genome shotgun sequence.
ACCESSION   DBNGZW010000133 DBNGZW010000000
VERSION     DBNGZW010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3944
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1307680"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..161
                     /locus_tag="AC16JC_03285"
     CDS             3..161
                     /locus_tag="AC16JC_03285"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR5163750.1"
                     /translation="MYEYRCTKCRKRFSESMPISQHGRKRPVCPKCGSRVVEPVFSAF
                     FAKTVRKS"
     gene            168..758
                     /locus_tag="AC16JC_03290"
     CDS             168..758
                     /locus_tag="AC16JC_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163751.1"
                     /translation="MFERLRDAVRAALDAATPPGNLRELARQMREAVVDAKVAVQEMR
                     AALARTEIELKVEAQRLSDAERRGRLAGEIQDQETVEVAERFAARHRERVAVLERKLA
                     AQREELSLAERDLAEMQGQLKSAERGGPAAPGGSGPMSSTEQAWRDLQAAGGARPGVD
                     LQDDLLKADMDRAALEQAADEQLDLLKQKLGKKKKE"
     gene            761..1756
                     /locus_tag="AC16JC_03295"
     CDS             761..1756
                     /locus_tag="AC16JC_03295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HIR5163752.1"
                     /translation="MLLTLVALLQQPSPAPADLTKRAHTYSIVAYDSATGDLGVAVQS
                     KFPNVGGIVPWARAGVGAVATQSLGNTAYGDDGLRLMGMGATAPEAMRIVMRSDQRPA
                     QRQVGMVDAHGNAVSWTGDSCFDWAGGRTLGADGRIVLGGKGAMIVGRNFAAQANIMV
                     SDQTVKNMADAFVRATGSLADRLMAALVAGQAGGGDKRGMESAALLVVRANGGYLGAN
                     DRYIDIRVYDDTNPIRELQRLYRLHQLYFFQSRPEDLIPITPDVVRQLEPILLSEPVN
                     QPQKWLDTPQGTANAKFLQALENFMYWENYDVRVRTDGKIDRVALEDILARRKKR"
     gene            1759..3123
                     /locus_tag="AC16JC_03300"
     CDS             1759..3123
                     /locus_tag="AC16JC_03300"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:1904680 - peptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HIR5163753.1"
                     /translation="MAAPSPVTVGTTGWFGHPRGLSTLFFTEMWERFSYYGMRGFLIL
                     YAVAPLANGGLGFDDRHGASIYKWYTSSVWLTPIVGGLIADRLLGQFRSVFLGGVIIA
                     LGHFSLAFKSLPSFYLGLTLIVVGTGLLKPNISTMVGSLYEPGDARRDAGFSIFYMGI
                     NIGAFLGIMGAGWLAQKIDWHIGFAAAGVGMTFGLIQYVLGRRRLQPALDRLATQRSR
                     AAAGEEARPATKGLWHELRHLTSVERKRIAVVFILFVFASLFWGAYEQAGSTLNLFAD
                     RYTNLDVFGYQIPSSWLQAVQPILVISLAPVLAWLWVRLGSREPSSPSKFALGLLAAG
                     LAFLLLVPAGAHAQSAPGVRVSPLWLVGAYFIEELGELCISPVGLSAVTKLAPVRIVS
                     LMMGVFFLSNWLGNLLAGWTAGFFSAMPLSQLFGAVAAVCLVSAVIMIVLIRPVKQLM
                     GGVH"
     gene            3200..>3944
                     /locus_tag="AC16JC_03305"
     CDS             3200..>3944
                     /locus_tag="AC16JC_03305"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5163754.1"
                     /translation="MAVVYLARDPRHDRRVAIKVLRPELAVALGPERFLREIRVAAHL
                     NHPHILPLHDSGEVGGFLYYVMPYVEGESLRGRLNREHQLPMDEAVRIARDVASALSY
                     AHEHGIVHRDIKPENVLLAGGGAEAVVTDFGIARAVSAAGKDDLTGAGVAIGTPAYMS
                     PEQASAATTIDGRSDIYSLGCLLYEMLVGHPPFSGGSQQEVLVRHTMDPVPTITAGRP
                     AVSVPLQRVVYRALAKSPADRYATAATFAE"
BASE COUNT          603 a         1269 c         1434 g          638 t
ORIGIN      
        1 cgatctacga atatcgctgc accaagtgcc gcaagcggtt cagcgagtcg atgccgatca
       61 gccagcacgg ccgcaagcgg cccgtctgcc ccaagtgcgg atcccgggtc gtggagccgg
      121 tgttttcggc tttcttcgcc aagaccgtcc ggaagagctg acccgcggtg ttcgagcgct
      181 tgcgggatgc cgtgcgggcc gcgctggacg cggcgacgcc gccggggaac ctgcgcgagc
      241 tggcccgcca gatgcgcgag gcggtggtgg acgccaaggt ggcggtgcag gagatgcggg
      301 cggcgctcgc gcgcaccgag atcgagctga aggtcgaggc gcagcggctg tccgatgcgg
      361 agcggcgcgg ccgcctggcc ggcgagatcc aggatcagga gaccgtggag gtcgccgagc
      421 gtttcgcggc gcgccatcgt gagcgcgtgg ccgtgctgga acgcaagctg gcggctcagc
      481 gcgaggagct gagtctcgcc gaacgcgatt tggcggagat gcaggggcag ctcaagtcgg
      541 ccgagcgagg cgggcccgcg gcccccgggg ggagcgggcc gatgagcagc acggagcagg
      601 cgtggcgcga cctccaggcg gcgggcgggg cacgacccgg cgtcgatctg caggatgatc
      661 tgctcaaggc cgacatggac cgggcggcgc tggagcaggc ggcggacgag caactggacc
      721 tgctgaagca gaaactcggg aagaagaaga aggagtaacg atgctcctga cgctcgtggc
      781 cctgctgcag cagccgagcc ccgcgccggc cgatctcacc aagcgcgcgc acacctacag
      841 catcgtggcc tacgattcgg ccaccggcga cctgggcgtg gcggtgcagt ccaagttccc
      901 gaacgtgggc ggcatcgtgc cgtgggcccg ggccggcgtg ggggcggtgg cgacgcagtc
      961 gctggggaac acggcgtacg gcgacgacgg gctgcgcctc atggggatgg gcgccacggc
     1021 gcccgaggcg atgcgcatcg tgatgcggag cgatcagcgg ccggcgcagc gccaggtcgg
     1081 gatggtggac gcgcacggca acgccgtgag ctggaccggc gactcctgct tcgactgggc
     1141 cggggggcgg acgctcggcg cggacggccg gatcgtgctg ggcgggaagg gcgcgatgat
     1201 cgtggggcgc aatttcgccg cccaggcgaa catcatggtc tccgaccaga cggtgaagaa
     1261 catggccgac gcgttcgtgc gggcgacggg ctcgctcgcc gaccggctga tggcggcgct
     1321 ggtggcgggg caggcgggcg gcggggacaa gcgcggcatg gagtcggcgg cgctgctcgt
     1381 ggtgcgggcc aatggcggct acctgggcgc caacgaccgc tacatcgaca tccgcgtgta
     1441 cgacgatacc aacccgatcc gcgagctgca gcggctgtac cggctgcacc agctgtactt
     1501 cttccagagc cgtcccgagg atctgatccc catcacgccc gacgtcgtgc ggcagctcga
     1561 gccgattctc ctgtccgagc cggtcaatca gccgcagaag tggctcgaca caccgcaggg
     1621 caccgccaac gcgaagttcc tgcaggcgct cgaaaacttc atgtactggg agaactacga
     1681 cgtgcgcgta cggaccgacg ggaagatcga ccgcgtggcg ctggaggata ttctggcgcg
     1741 ccggaagaag cgctgacgat ggccgcgccc tcgccggtca ccgtggggac caccgggtgg
     1801 ttcggtcatc cacgcggcct ctcgacgctc ttcttcacgg agatgtggga gcggttcagc
     1861 tactacggga tgcggggatt cctgatcctg tatgccgtcg ccccgctggc caacgggggg
     1921 ctcggcttcg acgaccggca cggcgccagc atctacaagt ggtacacgag ctccgtgtgg
     1981 ttgacgccga tcgtcggcgg actcatcgcg gatcgcctgc tgggccagtt tcggagcgtg
     2041 ttcctgggcg gcgtgatcat cgccctgggc cacttcagcc tcgcgttcaa gtcgctgccg
     2101 tcattctacc tcggactgac gttgatcgtg gtcgggaccg gcctcctgaa acccaacatc
     2161 agcacgatgg tcggctcgct gtatgaaccc ggggatgcgc ggcgcgacgc cggcttctcg
     2221 atcttctaca tgggcatcaa catcggcgcc ttcttgggca tcatgggtgc ggggtggctc
     2281 gcccaaaaga tcgactggca catcggcttt gcggctgccg gtgtggggat gacgttcggg
     2341 ctcatccaat acgtactcgg ccggaggcgc ctacagccgg cactcgaccg gttggccaca
     2401 caacgctccc gtgcagccgc tggcgaggaa gcgcgacccg cgaccaaagg gctatggcac
     2461 gaacttagac acctcacctc ggtcgagcgc aagcggattg cggtcgtgtt catcctgttc
     2521 gtcttcgcct ccctcttctg gggtgcgtac gagcaggcgg gatccacgct caacctgttc
     2581 gccgatcgct acacgaatct cgacgtcttc gggtatcaga ttccgtcttc ctggctccag
     2641 gcagtccagc cgatcctggt catcagcctc gcccccgttc ttgcgtggct ctgggtgcgt
     2701 ctcgggtcac gtgagccgtc gagtcccagc aaattcgctc tcggtctcct ggcagcgggc
     2761 ctcgccttcc tgttgctggt tcccgcaggc gcgcacgcgc agagcgcacc gggcgtgcgg
     2821 gtgagcccgt tgtggctcgt cggtgcctac ttcatcgagg agctgggtga gctgtgtatc
     2881 agcccagtgg gcctcagtgc ggtcaccaag ctcgccccgg tgcggatcgt cagtctgatg
     2941 atgggcgtct ttttcctgtc gaattggctg ggcaatctgc tggccggctg gacggccggg
     3001 ttcttcagcg cgatgccgct ctcgcagctg ttcggcgcgg tcgctgccgt gtgcctcgtg
     3061 tcggcggtga tcatgatcgt gctgatccgg ccggtaaaac agctgatggg gggagtgcat
     3121 tagcgatccc caggaggagc gcctccgcgc cgctcttgcc gagcggtata ccgtcgagcg
     3181 ggagctgggg cgtggaggca tggccgtcgt gtacctggcc cgggaccccc ggcacgatcg
     3241 gcgggtcgcc atcaaggtgc tgcgtcccga gctggcggtg gcgctcggcc ccgagcgctt
     3301 tctgcgcgag atccgcgtcg ccgcgcatct gaaccatccg cacatcctgc cgctgcacga
     3361 ctccggcgag gtcggagggt tcctctatta cgtgatgccc tacgtcgagg gcgagtcgtt
     3421 gcgcggccgg ctgaaccgcg agcaccagct cccgatggac gaagcggtgc gcatcgcgcg
     3481 cgacgtggcg agcgcgctga gctacgcgca cgagcacggc atcgtgcacc gggacatcaa
     3541 gccggagaac gtcctgctgg cgggcggggg agcggaagcg gtcgtcaccg acttcgggat
     3601 cgcccgggcg gtctcggcgg ccgggaagga cgacctgacc ggcgccgggg tcgcgatcgg
     3661 cacgccggcc tacatgagcc ccgagcaggc gagcgcggcg acgaccatcg atgggcgcag
     3721 cgacatctac agcctcgggt gtttgctcta cgagatgctg gtgggccacc cgccgttctc
     3781 cggcggatcg cagcaggagg tgctggtccg ccacacgatg gacccggtgc ccacgatcac
     3841 ggccgggcgg ccggcggtgt ccgtaccgct gcaacgtgtg gtgtatcgcg cgctcgccaa
     3901 atcgccggcc gaccgttacg ccaccgcggc gacgttcgcg gagg
//
LOCUS       DBNGZW010000134         8890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_77072, whole genome shotgun sequence.
ACCESSION   DBNGZW010000134 DBNGZW010000000
VERSION     DBNGZW010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8890
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_77072"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2726
                     /locus_tag="AC16JC_03310"
     CDS             <1..2726
                     /locus_tag="AC16JC_03310"
                     /inference="COORDINATES: protein motif:HMM:NF027232.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR5163755.1"
                     /translation="MRLARLPVVLILPLALAPPALRGQQPTGRRRAVPADTTKRDTTS
                     DSLSRFLGRFHYRALGPAAYSGRVTAIAVPRSAEPRPKTFYIGAAGGGVWKTTNGGVT
                     WSSVSSGLGSETIGDIAVAPSDSQIVWAGTGEKNSLRSQYWGDGVYKSTNGGKSWTKM
                     GLADSRAIGRIVIHPAGPDTVYVAALGHLWGPNSERGIYKTTDGGKTWSRILFVDDTT
                     GFVDLAMDPGDPNTLYAAAWHRLRWGGSHMEGVGKGSGIYKTTDGGKTWTRLTDSTRH
                     NGLPTDRMGRIGLALSAQNPKLVFAMIQVDRGVTDPAQGRFGGVFRSADGGATWTQVN
                     DLQAVPHYYYDAIFVDPTDSQHVFVATSPLLESKDGGRSFAPESLADVHVDHHALWID
                     PLDAAHRILGNDGGAYVTFDAGKAWWHMPLPIGQFYTVIVDSSLVPYQICGGLQDNGV
                     WCGPSATRDSSGIGDEDWYPVNGGDGMWVQIPPHDPWTVYSGWQFGNLSRLDLRTWQR
                     DDIQPESLDAGEESGYDYTWGWTTPILISSHDSGTVYLGGNRLFRLRQGGRDWEALGP
                     DMTRTPRRQPAPEVGHTSYHAIFSIAESPRTPAVLWTGSDDGLIWVSRDTARTWTNTT
                     TNFPRGAPTDCFVSTIVASAHEEGAAWVALDCHHRDDYRPHVWHTTDFGRTWTELVSG
                     LPPDRGSLTVLESPRNPRVLWVGTADGVYATVDGGAHWRRFGGTFPHVMVEALAMAWR
                     QHDLVVATHGRGVYVVGVGPLEEYSDSLLALPVQLFDVPPAYRFRWRDTRPSDGSASF
                     LAPNPPRGAVVGYYVRDLQEDGVKLVITSAAGDTVRTLTGSGIPGLQQVVWDLTSQRP
                     RPRGLGDPTSRDELRRVPPGNYVVHLTLGARKLERPIVVTELPPDRLGRIR"
     gene            2761..3303
                     /locus_tag="AC16JC_03315"
     CDS             2761..3303
                     /locus_tag="AC16JC_03315"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR5163756.1"
                     /translation="MRNVLSFGTAALLGLAACQKPPETPAQAQTRMDQEAAALRTQVQ
                     TLASSWERWTAAGQADSIAAIFVDQGYELPPNAPPVHGRDAIKAFQTQLASMGQSTIH
                     LSVDEVMANGPVGVARGAYEFELVPGPAAPAGTTALADTGKWVGALRQSDGQWRFTSM
                     MWNSNIPLPPPPAPTPSRRR"
     gene            3347..4096
                     /locus_tag="AC16JC_03320"
     CDS             3347..4096
                     /locus_tag="AC16JC_03320"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR5163757.1"
                     /translation="MAAALVGAVPRLGAQGSGVVTGRVTDAGSALGVNAAVVRPAGSA
                     RLALTDVHGEFRLADLPAGPLSIIVRAIGYRPIVVDVLLGAGESLRLANDVLALQPVP
                     IVLPGIDAPSDSTQRLALTRTGFYERRGRGFGVFADRNEIDRWNPHALSDILRHLNGV
                     QIKVNPDYGRTRPRIDLRPYLIEMRGCGNILFFLNGVSLGSSADSQFDLDFLAVPEDI
                     TAVEVYRGPSEIPLQFNSTNSLCGAVLIWSE"
     gene            4131..5369
                     /locus_tag="AC16JC_03325"
     CDS             4131..5369
                     /locus_tag="AC16JC_03325"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR5163758.1"
                     /translation="MSDPGDENLHDVADELTDLDRWRTETPGCAERIHLNNAGAALQP
                     RAVLEAVRAHLQRESEVGGYEAADEAAEAVQQSYEAVARLVGCAARNIAVVENATVGF
                     AQAVTAFDFRPGDVIVTSRADYVSNQLMYLSLARRLGVELRRAADLPEGGVDPESVRA
                     LLADGRVRLVAISWIPTNSGLIQAARAVGAVCAAAGVPYLIDACQAVGQLPVAVRDLE
                     CDYLSATARKFLRGPRGVGFLYVADRALARGDHPLLVDMRGADWTAADGYRLFDGARR
                     FESWESSCALVLGLGAAARYAETVGTIGHERARALAAILRERLAAIPGVRIMDRGSER
                     GAIVTIATDGADPEALKLRLRARGINTSVSSRDDAVLDMDEKGVRAVLRLSPHYYNTE
                     VEIDTAVAAVREETGRGAGV"
     gene            complement(5366..6088)
                     /locus_tag="AC16JC_03330"
     CDS             complement(5366..6088)
                     /locus_tag="AC16JC_03330"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AfsR/SARP family transcriptional regulator"
                     /protein_id="HIR5163759.1"
                     /translation="MEGLRLLTLGGLTIQAGDGLLTGATTRRHRVALLALVAVARNRG
                     LSREKIAAYLWPEKDAEHARHGLNQLVYAQRRHVEDGALFLGKKTLQLNPAIITADVW
                     EFEDALDSGAREAAVRLYAGPFLDGFFLRDAPEFDRWVEDQRRRLQRRCSDAIIGLAR
                     AATAAGDHVHALVWWRRAVELDPFDTDTVRHLVQASLIVGDRATALRDVQHHADLLRT
                     ELGLDPDPPLLQLIEQVRRASD"
     gene            6358..6936
                     /locus_tag="AC16JC_03335"
     CDS             6358..6936
                     /locus_tag="AC16JC_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163760.1"
                     /translation="MDGRFLHFPIVAACLGLTPAPLLAQTSAMPSAMSVSGRTTLLRP
                     GIPASDSELTAPVQARVVRGDALTGMRRFPAAEREYRAAADLAHREGHLPSFTLWHLA
                     SAYYYEGSPQRAAAVLEQLSAEAAQRGDLAVQALSLFNAAWLNGEGGRGRVAATEIAD
                     LSRLLRSPYMPATVRDHLNARLNPPSAVAVDH"
     gene            complement(6996..>8890)
                     /locus_tag="AC16JC_03340"
     CDS             complement(6996..>8890)
                     /locus_tag="AC16JC_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR5163761.1"
                     /translation="TRAPLESPGGAVSISGGERSVASATFRYAVRFSQRVGLAVSGDY
                     LGGHDWQFTDTTEQRLRQEAIAAGADPDTLRVGRRNPRLARAGGDLQLEWRPDTLTDI
                     RTTAGVAQAIDNIDLTSEVGAVQVQDWRYAFLQTRVRRGRLFGNAVYNTSDAGDTYVL
                     RTGTPLVDRSRTAALQGQYGSALGPIDVRYGADLRWTDPRTGGTIDGGYENHDRTLEL
                     GGYLHATAQVSARVALVGAARVDHHDRLNDLVLSPRAGIVYRVGSEHALRFTYNRAFT
                     SPDPSDLFADIVSDSLRGLPYAIRAGSIPQDGYSFERGGACGGICMRSPFAGGQYLPT
                     DATLLWPALVQILAADSIDLSGVPAPSAADVSTVLAAFRPGGAVIPVTLDAIQDVPAL
                     RRTITNTLELGYKGLTAGRVWLTVDVYRNQVTDPLGDRYDLTPSVFYDPATLAQYLSN
                     YLSPAEVAAAVPVAVSIPMGTITPREALYPTDVLFVRRQGGRYTLWGADIGANAALGH
                     GWSAAGTFSWVSSDSLAGVGRIGDLVLNAPRTKGSLSLGYQGGRWRWGATARAVSEFP
                     VASGVYNGRVAGYGLLDASVEYRLPGGRSSLTVDGQNLLDHRHQEIEGAPALGRLVVS
                     RLRVEF"
BASE COUNT         1343 a         3143 c         3108 g         1296 t
ORIGIN      
        1 ccatgcgcct cgcccggctg ccggtcgttc tcatcctgcc actcgcgctc gctccccctg
       61 cccttcgcgg gcagcagccg acgggtcgca ggcgggcggt tccggccgat actacgaagc
      121 gcgacacgac gtccgactcg ctcagccgct tcctcgggcg cttccactat cgcgcccttg
      181 gccccgcggc gtattcgggc cgggtgacgg cgatcgcggt gccgcgaagc gccgagccac
      241 gccccaagac gttctacatc ggcgcagcgg gcggcggcgt ctggaagacc acgaacggcg
      301 gtgtgacctg gtcgtcggtc tcctcgggac tgggcagcga gacgatcggc gatatcgccg
      361 tcgccccgtc cgacagccag atcgtgtggg ccggcaccgg cgagaagaac agcctgcgct
      421 cgcagtactg gggcgacggg gtctacaagt ccaccaacgg cggcaagagc tggacgaaga
      481 tggggctcgc cgacagccgc gcgatcggcc gcatcgtgat tcatccggcc ggtccggata
      541 cggtgtacgt ggccgccctg ggccacctgt ggggaccgaa ctccgagcgc ggcatctaca
      601 agaccaccga cggcggcaag acctggtcgc gcatcctgtt cgtggacgac acgacgggct
      661 tcgtggatct ggcgatggac ccgggcgacc cgaacacgct ctacgcggcg gcgtggcacc
      721 gcctgcgctg gggcggcagc cacatggaag gcgtcggcaa gggcagcggt atctacaaga
      781 cgaccgacgg cggcaagacc tggacccggc tcaccgactc gacccgccac aacggtctcc
      841 ccaccgatcg catggggcgc atcggcctcg cgctgtcggc gcagaacccg aagctcgtgt
      901 tcgcgatgat ccaggtggac cgcggcgtca ccgatcccgc gcaggggcgc ttcggcggcg
      961 tgttccgctc cgcggacggc ggcgcgacct ggacgcaggt caacgacctg caggcggtcc
     1021 cccactacta ctacgacgcg atcttcgtcg acccgaccga ttcccagcac gtattcgtgg
     1081 ccacgagccc gctgctcgag agcaaggacg gcgggcgcag cttcgcgccc gaatcgctcg
     1141 cggacgtgca cgtggatcac cacgcgctgt ggatcgaccc tctggacgcc gcgcatcgca
     1201 tcctggggaa cgacggcggc gcctacgtga cgttcgacgc cggcaaggcc tggtggcaca
     1261 tgccgctgcc catcgggcaa ttctacacgg tgatcgtgga cagctcgctc gttccctacc
     1321 agatctgcgg cgggttgcag gacaacggcg tgtggtgcgg gcccagcgcc acgcgcgact
     1381 cgagcggtat cggcgacgag gattggtatc ccgtgaacgg cggcgacggc atgtgggtgc
     1441 agatcccgcc gcacgatccc tggacggtgt attcgggctg gcagttcggc aacctgtcac
     1501 gcctcgactt gcgtacctgg cagcgcgacg acattcagcc cgagtcgctg gacgccggcg
     1561 aggagagcgg ctacgactac acctggggct ggaccacgcc gattctcatc tcctcccacg
     1621 actccggcac cgtctacctc ggcggcaacc ggctcttccg tttgcgccag ggcggccgcg
     1681 actgggaggc gctcggtccc gacatgacgc gcacgccccg ccgccagccg gcgcccgagg
     1741 tcggccacac gagctatcac gccatcttct cgatcgccga gagcccccgc acgcccgccg
     1801 tgctctggac cggcagcgac gacggcctga tctgggtgtc ccgcgacacc gctcggacgt
     1861 ggaccaacac cacgacgaac ttcccgcgtg gcgcgccgac cgactgtttc gtctccacca
     1921 tcgtggcctc ggcgcacgag gagggggcgg cctgggtcgc gctcgactgc catcaccgcg
     1981 acgactaccg gccgcacgtg tggcacacca ccgatttcgg gcggacctgg accgagctcg
     2041 tatcgggtct gcccccggat cgcggcagcc tgacagtgct cgagagtccg cgcaatccgc
     2101 gggtgttgtg ggtcgggacg gccgatggcg tgtacgcgac cgtggacggc ggagcgcact
     2161 ggcggcgatt cggcgggacg ttcccgcacg tgatggtcga ggcgctggcg atggcctggc
     2221 gccagcacga tctcgtcgtc gccacgcacg ggcgcggcgt ctacgtggtc ggagtcgggc
     2281 cgctggagga gtacagcgat tccctgctgg cgctgccggt ccagctgttc gacgtgccgc
     2341 ccgcctaccg gttccgctgg cgggacacgc gtcccagcga cggcagcgcc tcgttcctgg
     2401 cgcccaatcc gccgcgcggc gcggtcgtcg gctactacgt ccgggacctg caggaagacg
     2461 gggtcaagct ggtcatcacg tcggcagcgg gtgacacggt gcggaccctg accggctccg
     2521 ggatcccggg gctgcagcag gtcgtctggg atctcacgag tcagcggccg agaccgcgcg
     2581 ggctgggcga tccgacctca cgcgacgagc tgcgccgcgt gccgccgggc aactacgtcg
     2641 tgcacctcac gctcggcgcg cgcaagctcg agcggcctat tgtcgtcacc gaactgccgc
     2701 cggaccggct gggacggatc cggtagtcgt ggctggatca cactcaccaa gagggacgct
     2761 atgcgaaacg tactgagctt cggaacggcc gcgttgctgg gattggcagc gtgccagaag
     2821 ccgcccgaaa ccccggccca ggcgcaaacg cgcatggatc aggaggcggc ggcgttgaga
     2881 acgcaggtcc agacgctggc gagcagctgg gagcgctgga ccgccgccgg ccaggccgat
     2941 tcgatcgccg ccattttcgt ggatcaagga tacgagctgc cgccgaacgc gccgccagtt
     3001 cacgggcggg atgccatcaa ggcgttccag acccaactcg cctcgatggg ccagtcgacg
     3061 attcatctca gcgtggacga ggtgatggcc aacggcccgg tcggcgtggc ccgcggcgcg
     3121 tatgaattcg aactggtgcc cgggccggcc gcaccggccg ggacgaccgc gctcgcggac
     3181 accggcaagt gggtgggcgc cctgcgccag agcgacgggc aatggcgctt cacgtcgatg
     3241 atgtggaaca gcaatatccc gttgccgccg ccgcccgcac cgacgcccag tcgcagacgg
     3301 tagagatgtg acacgtcgag cggtctgcag cgcaggcatc gccctgatgg ccgcggcgct
     3361 cgtcggggcg gtgccacggc tcggtgcaca gggcagcggg gtcgtgacgg ggcgggtcac
     3421 ggacgcggga tcggcgctcg gggtgaacgc cgccgtcgtg agaccggcgg ggagcgcgcg
     3481 gctcgcgctc acggacgtac acggagagtt tcgcctggcc gacctccctg cgggcccgtt
     3541 gagcatcatc gtccgggcga tcgggtaccg tccgatcgtg gtggacgtcc tcctgggggc
     3601 gggcgagtcg ctccggctgg ccaacgatgt cctggcgctg cagcccgtgc cgatcgtgct
     3661 tcccggcatc gacgccccca gcgactccac ccagcgcctc gcgctcaccc gaacgggctt
     3721 ctatgagcgc cgcggccggg gattcggcgt cttcgccgac cgcaacgaga tcgaccgctg
     3781 gaacccgcat gccctgagcg acatcctgcg gcacctcaac ggcgtgcaga tcaaggtcaa
     3841 tcccgactac ggacgcacgc ggccgcggat cgacctgcgt ccctacctca tcgagatgcg
     3901 cggctgcggc aacatcctgt tcttcctcaa cggcgtgtcg ctggggagct cggcggactc
     3961 gcagttcgat ctggacttcc tggcggtgcc ggaggacatc acggcggtcg aagtgtaccg
     4021 cggaccttcg gagatacccc tgcaattcaa ctcgacgaac agcctctgcg gggcggtgct
     4081 catctggagc gagtgaccgt ccgcccccgg tcagcatgcg gtagtggcgc ttgagcgacc
     4141 ctggcgatga gaacctgcac gatgtggcgg atgaattgac cgacctcgac cgctggagga
     4201 ccgagacccc gggctgcgcc gagcgcatcc acctgaacaa cgccggcgcg gccctgcagc
     4261 cgcgggcggt gctcgaagcc gtccgcgcac acctgcaacg cgagagcgag gtcggaggtt
     4321 acgaggccgc cgacgaggcc gccgaggcgg tgcagcagag ctacgaggcg gtggcgcgcc
     4381 tcgtcggctg cgcggcccgc aacatcgcgg tcgtcgagaa cgcgaccgtc gggttcgcgc
     4441 aggccgtgac ggctttcgac ttccgtcccg gcgacgtcat cgtgaccagc cgggcggact
     4501 atgtctcgaa ccagctgatg tacctgtcgc tggcgcggcg cctcggcgtg gagctgcgcc
     4561 gcgcggcgga cctccccgaa ggcggcgtcg atccggagag cgtccgagcg ctgctggcgg
     4621 acggccgggt gcgtctcgtg gcgatcagct ggattccgac caattcggga ctgatccagg
     4681 cggcgcgcgc cgtcggtgcg gtgtgcgcgg ccgccggcgt gccctatctc atcgacgcat
     4741 gccaggcggt ggggcagctg ccggtcgccg tgcgtgacct ggagtgtgac tatctgtcgg
     4801 ccacggcgcg gaaattcctg cgcggccccc gcggcgtcgg cttcctctac gtcgccgacc
     4861 gtgcgctcgc gcgcggcgat catccgttgc tggtggacat gcgcggcgcc gattggaccg
     4921 ccgccgacgg ctaccgcctg ttcgatggcg cccggcggtt cgagagctgg gagtcctcat
     4981 gcgccctggt gctcggcctg ggggcggccg cgcgctacgc ggagaccgtg ggtaccatcg
     5041 gacacgagcg ggcgcgcgcc ctcgccgcca tactgcgcga gcgcctcgcg gcgatccccg
     5101 gcgtgcggat catggaccgg ggcagcgagc gcggggccat cgtcaccatc gccaccgatg
     5161 gcgcggaccc ggaggcgctc aagctgcggt tgcgggcgcg gggaatcaac accagcgtct
     5221 cgagtcgcga cgacgcggtg ctcgacatgg atgagaaagg cgtgcgcgcg gtgctgcgcc
     5281 tgtccccgca ctactacaat acggaagtcg agatcgatac ggccgtggct gccgtgcggg
     5341 aggagacggg cagaggcgcc ggcgtctagt cgctcgccct gcgcacctgt tcgatcagtt
     5401 gcagcagcgg tggatccgga tccaggccga gctcggtgcg aagcaggtcc gcatggtgct
     5461 ggacgtcgcg cagcgccgtg gcgcgatcgc cgacgatcag cgacgcctgg accaggtgcc
     5521 gcacggtgtc ggtgtcgaac ggatccagct ccaccgcccg gcgccaccag accagcgcgt
     5581 ggacatggtc gcccgccgcc gtcgcggctc gcgcgagccc gataatcgcg tcgctgcagc
     5641 gccgctgcag cctgcggcgc tggtcctcga cccatcggtc gaactccgga gcatcccgca
     5701 ggaagaagcc gtccaggaag ggtcccgcat acagtcggac ggccgcttcg cgggcgccgc
     5761 tatcgagcgc gtcctcgaat tcccagacgt cggcggtgat gatcgcgggg ttgagctgga
     5821 gcgttttctt cccgaggaag agcgccccgt cctcgacgtg gcgtcgctgc gcgtagacca
     5881 gctggttcaa cccgtggcgc gcgtgctccg catccttctc gggccacagg taggcggcga
     5941 tcttctcccg actcagcccg cggttacgcg cgacggcgac cagcgcgagc agcgcgacgc
     6001 gatgacgacg cgtggtcgcg ccggtcagca gcccgtcgcc ggcctgaatc gtcaagcctc
     6061 cgagggtgag cagacgaagc ccctccatcc cgatcactcc cgtcccgctc ctccggtaga
     6121 ccctcgcccg tcgccgggcc gcggggtcca atacaccatc cgctaggcaa tttggagtca
     6181 atcaacggtc gtctcttcgt ccggacggcg ctcctccccc gggagcagcg gctgattcgc
     6241 cggtgatccc cgggtgaccg ggggctgacc gggcgctcct accatggtgc tcgaatcgaa
     6301 tgtcgccaga cccggtggag taccgggaca cggtcgacga tcacccggag gtggagaatg
     6361 gacggccgat tcctgcattt cccgatcgta gcagcctgcc tgggcctcac ccccgcgccc
     6421 ctgctggcgc agacgagcgc catgccatcg gcgatgtccg tgtcagggcg gaccaccctc
     6481 ctgcgacccg gcatcccggc gtccgacagc gagctcaccg cgccggtgca ggcacgggtg
     6541 gttcgcggtg acgcgctgac cggcatgcga cgcttcccgg cggcggagcg ggagtatcgt
     6601 gccgcggccg acctcgcgca ccgcgagggt cacctgccga gcttcacgct gtggcatctc
     6661 gccagcgcct actactacga gggcagtccg cagcgcgccg ccgccgtgct cgagcagctg
     6721 tcggccgagg cggcgcagcg cggtgatctg gcggtgcagg cgctctccct gttcaacgcc
     6781 gcgtggctga acggcgaggg cggccggggg cgggtggcgg cgacggagat cgccgacctg
     6841 tccagactgc tgcgctcacc gtacatgccg gcgacggttc gcgatcacct caatgcccgg
     6901 ttgaatccac ccagcgccgt cgccgtcgac cactgaccgc ggttcccggg tcgtcggagc
     6961 gggttgcgcg tatcgcgcag cccgctccga ggccttcaga actccacccg cagccgcgac
     7021 accaccaggc gtcccagcgc cggcgccccc tcgatctcct ggtgacggtg atcgagcagg
     7081 ttctggccgt ccaccgtgag cgagctccgg cccccgggaa gacggtactc gacgctcgcg
     7141 tctagcaggc catagcccgc gacccggccg ttgtacacgc ccgaggccac ggggaactcc
     7201 gacacggccc gcgccgtcgc gccccaccgc cagcggccac cctggtatcc gagggacagg
     7261 gaccctttcg tgcgcggggc attcaacacg agatcgccga tccgccccac gccggccagg
     7321 gaatcgctcg acacccagga gaaggtgccg gccgccgacc atccgtgccc caacgccgcg
     7381 ttcgcaccga tgtctgcgcc ccagagggtg tagcgacccc cctgccggcg cacgaacagc
     7441 acgtcggtgg gatacagcgc ctcgcgcggc gtgatggtgc ccatggggat gctgaccgcc
     7501 accggaacgg cggcggcgac ctccgccggt gacaggtagt tcgacagata ttgcgccagc
     7561 gtcgcgggat cgtagaacac gctgggcgtg aggtcgtagc gatcacccaa cgggtccgtg
     7621 acctggttgc ggtagacgtc cacggtgagc cacacgcggc cggccgtgag tcccttgtat
     7681 cccagctcca gcgtgttcgt gatggtgcgg cgcagcgccg gcacgtcctg gatcgcgtcg
     7741 agcgtgaccg ggatgaccgc gccgccgggt ctgaacgccg ccagcaccgt ggacacatcc
     7801 gccgcggagg gcgccggcac gccggacaga tcgatgctat cggccgcgag gatctgcacc
     7861 agggcaggcc acaggagcgt ggcatcagtg gggaggtact ggcccccggc gaacggcgac
     7921 cgcatgcaga taccgccaca ggcccccccc cgctcgaacg agtagccgtc ctgcggaatc
     7981 gatcccgctc tgatggcata cggcaaaccg cggaggctgt cgctgacgat gtccgcgaac
     8041 agatcgctcg gatcgggcga ggtgaacgcc cggttgtagg taaagcgcag cgcatgctcg
     8101 ctgcccaccc ggtacacgat tccggcacga ggcgacagga cgagatcgtt cagccggtcg
     8161 tggtggtcca cgcgcgcggc cccgaccaac gcaacccggg cgctcacctg cgcggtggcg
     8221 tggaggtagc cgcccagctc gagcgtccga tcgtggttct cgtacccgcc gtcgatcgtg
     8281 ccgcccgtgc gcgggtcggt ccagcgcaga tcggcgccgt aacggacatc gatcggcccg
     8341 agcgcgctcc cgtattgtcc ctggagcgcg gccgtgcgcg accggtccac caacggggtg
     8401 ccggtgcgga gcacgtaggt gtcgcccgca tcgctcgtat tgtagacggc gttgccgaac
     8461 agccgcccgc ggcgcacgcg ggtttgaaga aacgcataac gccagtcctg cacctggacg
     8521 gccccgactt ccgaggtcag gtcgatgttg tcgatcgcct gggccacccc cgcggtcgtc
     8581 cggatgtccg tgagggtatc cggccgccac tccagctgca ggtcgccacc ggcgcgcgcc
     8641 aggcgcggat tgcgccggcc gacgcgcagc gtgtcggggt ccgcgccggc ggcgatcgct
     8701 tcctgccgca ggcgctgctc ggtcgtgtcc gtgaactgcc agtcgtgccc gccgaggtag
     8761 tctcccgaga cggcgagccc gacccgctga ctgaaccgga cggcgtagcg gaacgtcgcg
     8821 gaggcgaccg agcgctcccc gccgctgatc gacaccgccc cgccggggga ctcgagcggt
     8881 gcgcgcgtga
//
LOCUS       DBNGZW010000135         7216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_898441, whole genome shotgun sequence.
ACCESSION   DBNGZW010000135 DBNGZW010000000
VERSION     DBNGZW010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7216
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_898441"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..811
                     /locus_tag="AC16JC_03345"
     CDS             <1..811
                     /locus_tag="AC16JC_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR5163762.1"
                     /translation="REFLHKTQVRFEAGSVPRLDVLKAQVDLAQAENALLANQRDVAN
                     ARAALNRLLGRPLGAGIEAADKLAIPPDLSPLDSLVTIALARRPELLGMARQRAGAGA
                     ATALAQQYWLPDLDLSVEKNLSQGTPGTYTTGIGISVPLFFWNHQRGEVAESKHRERE
                     LDAAYLDLEAEVEEDVRTTYATAVTALQQAVYLRDQLLPEARRAYEIALTSYGLGGSS
                     TLDVLDARRTLLDAESQYAEALGAANDARADLERAIGAPLDEHSTGARHDH"
     gene            801..1922
                     /locus_tag="AC16JC_03350"
     CDS             801..1922
                     /locus_tag="AC16JC_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008435165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5163763.1"
                     /translation="MITDRRAWILAVLCAACGRGGAKVDADHGAAFGLTATQRARIHV
                     DPVSVADFHPTVVVSGTVAFDGDHSTQVLAPISGPVSRILVEPGARVAAGQPLAYVSS
                     PDFAAALAAYRKAQAAATNALRVAARDSALYANDALARQELEQAQTDAAGAVADRDAA
                     LDQIRAIGLDSITVNAIRQGQSIPIPQGVIRAPLPGTVVERLITPGQLLLAGQTPCFT
                     VARLGTMWVMGNVFEQDLPDVAVGDVADVQPTGGERVFHGRVDYVGALVDAETRATAV
                     RVVTENPGEGLKKDMYVRVAIHSRHARRGLLVPTSSVLRDDQNLPFVFLVKPDSSFER
                     RTVRLGSRVGERFEIASGLVAGDRVVTEGALFLQFAENQ"
     gene            1922..5044
                     /locus_tag="AC16JC_03355"
     CDS             1922..5044
                     /locus_tag="AC16JC_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5163764.1"
                     /translation="MDQAPTPPDELRTASFINRIVAVSLRQRFLVGLLTLLLIGVGVW
                     SFSRLPVDAYPDLSPTMVEVITQWPGHAAEEVERLITVPLEVEMNGLPRMRIIRSVSL
                     YGLSDVVMTFADGTDDYFARQQVFERLPDAELPDGVSPSVSPLFSPSGLVYRYVVESP
                     DRSPRELKIINDWVIGRQYKSVPGVADASLFGGETFQYQVLLDPTRLAGAGLSVADVE
                     NALAQNNGNAGGGFYSEGGQFYYIRGLGRLESLEDIGSVVLANRNGVPILVKDVGEVV
                     AGHAPRLGQFGFNRTDDAVEGVILMRKGEQAQTVLKGVAAKTRELNQSILPKDVKVHT
                     YYDRSDLIALTTHTVEGNLARGIILVLVVLVFFLYDVRSALIVGVTIPLSLLFAFLWL
                     DLRDIPANLLSIGAIDFGILVDSAVIMVENIHRQVALRYGTDYRLHEVIVDAAAEVDR
                     PIFYSVAVIVAGFLPIYVLSGPSGKLFKPTADTMIFALIGSLLVTLTLLPMLCAWLLR
                     RGIKERRNPVFERIKRWYERTLDWCLARPRATIATSGGLFALGLLLTPAIGAEFMPHL
                     DEGAVWVRATMPYTISLEESAKIVPQIRAILMSFPQVTDVADEHGRPDDGTDPTGFFN
                     AEFYVGLKPYPEWSGPIRTKAQLIAAIDQRLESFPGIIFNYTQPAEDAVDEAETGLKS
                     ALDVKVFGPDLDTLQTIGKQIKRVLQNVAGVSQLTLVEELGQPSLTVTPDRARIARYG
                     LNVADINNLIEAAVGGGAATQIVQGERLFDLVVRLEPQFRETPEAIGNIVVATPGGQH
                     IPLKELATIQVANGASFIYRQDNSRFIGVQYSVEGRDLAGTVQDAQRRVAAQVHAPPG
                     YRVVWGGEYQEYTASRSQLRVIVPLTLLLILLILFALYGNMKFPLIAVGGVVLSAPLG
                     GLLMLWITGTPFSVSSGIGFLALFGVSVQTAVVYVSYANELRKGGMDIANAAREAALL
                     RLRPIMMTAIVAALGLLPAAMAHGVGTDSQRPFAMVIVGGMMSELLISILLMPVLYAL
                     VARSGDRLEV"
     gene            5044..5652
                     /locus_tag="AC16JC_03360"
     CDS             5044..5652
                     /locus_tag="AC16JC_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163765.1"
                     /translation="MRNQVSVFVLLYVGAVRLGAQTSLHATVAARYTSTLVHDSIVTP
                     LDVRPDVAPALTAAVDLPLTAPWRLELLADLSTSTLRRHDAGGTAPITRVWTLGVSLG
                     LRRQLNPWLEGRAAVGGLKYLPAASIGLFSAGSGGVMPYGSFALDAAPAVLAQRRLAL
                     EVAADVHRFFTPALRNAGFTDARLVYRLSVGLRLDLRRGRAS"
     gene            5649..6293
                     /locus_tag="AC16JC_03365"
     CDS             5649..6293
                     /locus_tag="AC16JC_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163766.1"
                     /translation="MRCGRWVVLGTVLLAAACTDPLVPSRTAIYPFADAFGDVFHWPA
                     DRLPVHVYADPRGDLSALVGPAGDLWARQLLYGELKVVMVADSNRADVIVRWADSVPP
                     AATPDPGPPVQACGGVTQVTLDSTGQALAAPFETEISILAGTVFTPEQVAACVRRTTL
                     HELGHSFGLFREAADTLSAMRSPPLVAEPSAIDRNTIQVLYHTPVTLAPPPRRP"
     gene            6390..>7216
                     /locus_tag="AC16JC_03370"
     CDS             6390..>7216
                     /locus_tag="AC16JC_03370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HIR5163767.1"
                     /translation="MSSRRQEALDYHSQGRPGKIQVSPTKPFKNQRDLSLAYTPGVAE
                     PCLEIAKRPEDAYLYTTRGNLVAVVSNGTAVLGLGDIGALASKPVMEGKGILFKAFAD
                     IDVFDLEVGSTDPEDLIRFCQLLEPTVGGINLEDVRSPDCFYVEERLRETLSIPVFHD
                     DQHGTAIITGAALMNALELVEKDAAEVQVVFAGAGAAALATAEHYVRLGVQRAHIALC
                     DVHGPLHDGRTDLNPYQRRFARTLRARTLAELFRGADVFVGLSTGGIVSGDMIKDMA"
BASE COUNT         1101 a         2517 c         2396 g         1202 t
ORIGIN      
        1 acgggagttc ctgcacaaga cgcaggtccg ctttgaggcg ggctcggtgc cacgactgga
       61 cgtgctgaaa gcccaggtgg acctcgccca ggcggaaaac gccctgctcg ccaaccagcg
      121 cgacgtcgcc aatgccaggg ccgccctcaa ccggctgctg gggcgtcccc tgggcgccgg
      181 gatcgaggca gcggacaagc tcgcgattcc gccggatttg tcccccctcg attcgctcgt
      241 gaccatcgcg ctggcccgcc gacccgagct cctcgggatg gcgcgacagc gtgccggcgc
      301 cggtgccgcc accgccctgg cgcagcagta ctggctgccg gatctggacc tatcggtcga
      361 aaagaacctg agccagggga cgcccgggac ctacacgacg ggcatcggca tcagcgtgcc
      421 cctgttcttc tggaaccatc aacgcggtga agtggcggag tcgaagcatc gcgagcggga
      481 gctcgatgcc gcctacctcg atctcgaagc ggaggtcgag gaggacgtgc gcacgaccta
      541 cgccaccgcc gttacggcac tgcagcaggc ggtgtacctg cgggaccagt tgctccccga
      601 agcccggcgg gcctacgaga tcgccctgac cagctacggt ctcggcggct cgtcgaccct
      661 cgacgtgctc gacgcccgtc ggacgctgct cgacgccgaa agccagtacg ccgaagcgct
      721 gggcgcggcc aacgacgccc gcgccgatct ggagcgtgcg atcggcgccc ccctggacga
      781 gcattccacc ggagcccgcc atgatcactg accgccgcgc ctggattctc gccgtgctct
      841 gtgccgcctg cgggcggggc ggcgcaaagg tcgacgccga tcacggcgcc gcgttcggtc
      901 tcaccgccac gcagcgcgcc cggatccacg tggatcccgt cagcgtcgcg gatttccacc
      961 ccacggtcgt ggtcagcggc acggtggcct tcgacggcga ccacagcacc caggtgctcg
     1021 cgccgatctc cggaccggtg agccgcatcc tggtggagcc gggagcgcgg gtcgccgccg
     1081 ggcaaccgct ggcctatgtg tcctcgccag acttcgcggc cgcgctcgcc gcctatcgca
     1141 aggctcaagc ggcggccacc aacgcgctgc gcgtcgccgc ccgcgactcg gccctgtatg
     1201 cgaacgacgc cctggccagg caggagctgg agcaggcgca aaccgacgcg gcgggcgccg
     1261 tcgccgaccg cgatgcggcg ctcgatcaga tccgcgccat cggcctcgac tccatcaccg
     1321 tcaacgcgat tcgtcagggc caatcgatcc cgatcccgca gggcgtgatc cgggcgccgc
     1381 tccccggcac cgtggtggag cgcctcatca caccaggcca gctgctcctg gcgggccaga
     1441 ccccgtgctt caccgtggcg cggctcggga cgatgtgggt gatgggaaac gtattcgagc
     1501 aggatctgcc cgacgtggcc gtgggcgacg tcgccgacgt gcagccgacc ggcggagagc
     1561 gggtcttcca cgggcgggtg gactatgtgg gcgcgctggt ggacgcggaa acgcgcgcga
     1621 cagcggtgcg ggtagtgacc gagaacccgg gggaggggct gaaaaaggac atgtacgtcc
     1681 gtgtcgcgat tcactcgcgt cacgcgcgac ggggcctgct cgtccccacc tcgtccgtgc
     1741 tgcgcgacga tcagaacctg cccttcgtct tcctggtcaa gcccgacagc tcgttcgagc
     1801 gacgcaccgt gcgcctcggt tcgcgtgtgg gagaacggtt cgagatcgcg tccggcctcg
     1861 tcgcaggtga ccgcgtcgtc acggagggcg cgctgttcct gcagttcgcg gagaatcagt
     1921 aatggaccag gcgcccaccc cacccgacga gctccgcact gcgtcgttca tcaaccggat
     1981 cgtcgcggtc tcgctgcggc agcgttttct cgtcgggctc ctgaccctgc tgctgatcgg
     2041 tgtcggggtc tggtcgttct cgcggcttcc ggtggatgcg taccccgacc tctcgccgac
     2101 catggtggaa gtcatcaccc aatggccggg gcacgccgcc gaagaggtgg agcgcctgat
     2161 caccgtgccg ctggaagtcg agatgaacgg cctgccgcga atgcggatca tccgctccgt
     2221 ctcgctgtac ggtctctcgg acgtcgtgat gacgttcgcc gacggcaccg acgactattt
     2281 cgcccgccag caggtgttcg agcgactccc cgacgcggag cttcccgacg gcgtgtcccc
     2341 ctcggtgtct ccgctcttct cgccgtcggg gctggtgtac cgatacgtgg tggagagccc
     2401 cgaccgttcg cccagagagc tgaagatcat caacgactgg gtcatcggac ggcagtacaa
     2461 gtcggtcccg ggtgtggccg acgcatcact gttcggcgga gagaccttcc agtaccaggt
     2521 cctgctcgat ccgacgcgcc tcgccggcgc cggcctgtcg gtggcggacg tcgagaacgc
     2581 gctggcgcag aacaacggca atgccggcgg cggcttctat tccgaaggcg gccagttcta
     2641 ctacatccga ggactcggac ggctcgagag tctcgaggac atcggcagcg tcgtgctcgc
     2701 aaaccgcaac ggcgtgccca tcctcgtgaa ggacgtcggg gaggtggtcg cggggcatgc
     2761 cccccgcctc gggcaattcg gcttcaatcg gacggacgac gccgtcgagg gcgtcatcct
     2821 gatgcgcaag ggcgagcagg cgcagaccgt gctgaagggc gtcgccgcca agacccggga
     2881 gctcaaccaa tccatcctcc cgaaagacgt caaggtccac acctactacg accgcagtga
     2941 cctgatcgcc ctgaccacgc atacggtgga gggaaacctg gcgcgcggga tcatcctggt
     3001 cctcgtggtc ctggtcttct tcctctacga cgtccgctcg gcgctgatcg tcggggtgac
     3061 catccccctg tcattgctgt tcgcgtttct ctggctcgac ctacgcgaca tccccgcgaa
     3121 tctgttgtcc atcggagcga tcgacttcgg gatcctggtg gacagcgcgg tcatcatggt
     3181 cgagaacatc caccggcagg tcgccctgcg gtacggaacc gactaccgcc tgcatgaggt
     3241 catcgtcgac gccgcggccg aagtggaccg gcccatcttc tattccgtgg cggtgatcgt
     3301 ggccggcttc ctgccgatct acgtgctctc cgggccgtcg ggcaagctgt tcaagcccac
     3361 cgccgacacg atgatcttcg cactgatcgg ctcgctgctg gtcacgctga cgttgttgcc
     3421 gatgctctgc gcctggctgt tgcggcgcgg catcaaggag cgccgcaacc cggtgttcga
     3481 gcggattaag cgctggtacg agcggacgct cgactggtgc ctcgcgcggc cccgcgcgac
     3541 gatcgcgacg tccggcgggc tgttcgcgct cggcctgctg ctcaccccgg cgatcggtgc
     3601 ggagttcatg ccgcatctcg atgagggcgc cgtctgggtg cgcgccacga tgccctatac
     3661 gatctcgctc gaggagtcgg cgaagatcgt tccgcagatc cgcgccatcc tcatgtcgtt
     3721 cccccaggtg acggacgtgg cggacgagca cggtcgcccc gatgacggca ctgaccccac
     3781 cggcttcttc aacgccgagt tctacgtcgg cctgaagccg tatccggagt ggtccggccc
     3841 gatcagaacc aaggcgcagc tgatcgcggc catcgaccag cggctcgaat cgttcccggg
     3901 aatcatcttc aactacacgc aacccgccga ggacgcggtg gacgaggcgg agacggggtt
     3961 gaagagcgca ctcgacgtga aggtgttcgg gcccgacctc gataccctgc aaaccatcgg
     4021 caagcagatc aaacgcgtgc tccagaacgt ggcgggggtc agtcagctca cgctggtcga
     4081 agagctggga cagcccagtc tcaccgtcac ccccgaccgg gccaggatcg cgcgctacgg
     4141 gctcaacgtc gcagacatca acaacctcat cgaagcggcg gtcggcggcg gcgcggccac
     4201 acagatcgtc cagggagaac ggctgttcga tctggtggtg cggctcgagc cgcagtttcg
     4261 ggaaacaccc gaggcgatcg ggaacatcgt ggtggcgacc cccggcgggc agcatatccc
     4321 gctcaaggag ctggcgacca ttcaggtggc caacggcgcc tcctttatct atcgccagga
     4381 caactcccgc ttcatcggcg tgcagtactc ggtcgaagga cgcgacctcg cgggcacggt
     4441 acaggacgcc caacggcggg tggcggcgca ggtgcacgcg ccgccgggct accgcgtcgt
     4501 atggggagga gagtatcagg aatacacggc gtcacgcagc cagctgcggg tgatcgtgcc
     4561 cctcaccctg ctgctcatcc tgctgatcct gttcgcgctg tacggcaaca tgaagttccc
     4621 cctgatcgcg gtggggggcg tggtgctgtc cgctccgctg ggggggctcc tgatgttgtg
     4681 gatcacggga actcctttct cggtgtcctc cggcatcggc ttcctcgcgc tgttcggggt
     4741 ctcggtgcaa accgccgtgg tgtacgtttc gtacgccaat gagctgcgca agggcgggat
     4801 ggacatcgcc aacgccgccc gcgaggcggc cctgctccgt ctgcgtccga tcatgatgac
     4861 cgctatcgtc gccgccctgg ggctgctccc cgcggccatg gcgcatggcg tcggaaccga
     4921 ttctcagcgt ccgttcgcca tggtgatcgt cggcgggatg atgtcggagc tgttgatcag
     4981 catcctcctg atgcccgtcc tgtacgccct ggtcgcgcgc agcggggatc gcctggaggt
     5041 gtgatgagga accaggtttc ggtgttcgtg ctgctgtacg tcggcgcagt ccggctcggc
     5101 gcccagacgt cgctgcacgc caccgtcgcc gcacgctaca cgtcgaccct ggtgcacgat
     5161 tcgatcgtga cacccctcga cgtgcggccc gacgtcgcgc ccgcgctcac ggccgcagtg
     5221 gatctgccgc tcaccgctcc ctggaggctc gagctgctcg ccgatctctc gacgagcacc
     5281 ctgcggcgtc acgacgcggg cggtaccgcg cccatcaccc gggtgtggac gctcggcgtg
     5341 tcgctggggt tgcgccggca gctcaatccg tggctcgagg ggcgcgccgc ggtgggtggt
     5401 ttgaagtacc tgccggccgc ctcgatcggt cttttcagcg ccggcagcgg cggcgtgatg
     5461 ccgtacggct cgttcgcgct ggatgccgcg ccggccgtcc tcgcacagcg tcgcctggcg
     5521 ctcgaggtgg ccgccgacgt gcatcggttt ttcaccccgg cgctgcgcaa cgccggattc
     5581 accgacgccc ggctcgtgta tcgcctcagc gtcggcctgc gactggacct gcggcgcggg
     5641 agggcatcgt gaggtgtggg cgctgggtgg tgctgggtac cgtgctgctg gccgccgcct
     5701 gcaccgatcc cctggtaccg agccgcacgg cgatctatcc gttcgccgat gccttcggcg
     5761 acgtgttcca ttggcccgcc gaccggctcc ctgtgcacgt ctacgccgat ccccgcggcg
     5821 atctctccgc tctggtcggc ccggcgggcg atctctgggc gcgtcagctg ctctacggtg
     5881 agctcaaggt ggtcatggtg gcggactcga accgcgccga cgtgatcgtg cgctgggccg
     5941 acagtgtacc gcccgcggct acacccgatc cgggtccgcc ggtccaggcg tgcggcggcg
     6001 tcacacaagt caccctggac agcactggac aagcgcttgc cgcgccgttc gagaccgaga
     6061 tcagcattct tgccggcacc gtattcacgc ccgagcaggt ggcggcgtgc gttcgccgta
     6121 cgacgctgca cgagctcggg cattccttcg ggctgttccg ggaagcggcg gatacgctct
     6181 ccgccatgcg ctcgccgccg ctcgtcgccg agccgagcgc catcgatcgc aacacgatac
     6241 aggtgttata tcacacaccg gtaactctcg cgccgccgcc gcgcagaccg taagccccac
     6301 gtacgcctgg gaatacggcg cttcggcttg acacccccgc cccgtctgcc tagcataacc
     6361 ccttccccga aaaggacttc tggatctcga tgtccagccg ccgccaggag gccctcgact
     6421 atcactcgca gggccgccct gggaagatcc aggtctcgcc gaccaagccc ttcaagaacc
     6481 agcgcgacct gtccctcgcc tacacgccgg gggtggcgga gccgtgcctg gagatcgcca
     6541 agcgccccga ggacgcgtac ctgtatacga cccggggcaa cctcgtggcc gtggtctcga
     6601 acggcaccgc ggtgctcggc ctgggcgaca tcggggcgct cgcctcgaag ccggtcatgg
     6661 aaggcaaggg gatcctcttc aaggcgttcg ccgacatcga cgtgttcgac ctggaagtcg
     6721 ggtccaccga tcccgaagac ctgatccggt tctgccagct gctcgagccg accgtcggcg
     6781 gcatcaacct cgaagatgtg cgctccccgg attgcttcta cgtggaagag cggctgcggg
     6841 agacgctgtc cattcccgtg ttccacgacg accagcacgg caccgcgatc atcaccggcg
     6901 ccgcgctcat gaacgccctg gagctcgtcg agaaggacgc ggccgaggtg caggtggtct
     6961 tcgcgggcgc cggcgccgcc gccctcgcca ccgcggagca ttacgtccgc ctgggcgtgc
     7021 agcgcgcgca catcgcgctg tgcgacgtcc acgggccgct gcacgacggc cggaccgacc
     7081 tgaatcccta ccagcgccgc ttcgcccgga cgctgcgcgc ccggacgctg gccgagctgt
     7141 tccgcggcgc cgacgtgttc gtcggactgt cgacgggcgg gatcgtcagc ggcgacatga
     7201 tcaaggacat ggcgga
//
LOCUS       DBNGZW010000136         3210 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_625421, whole genome shotgun sequence.
ACCESSION   DBNGZW010000136 DBNGZW010000000
VERSION     DBNGZW010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_625421"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="AC16JC_03375"
     CDS             <1..806
                     /locus_tag="AC16JC_03375"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140905 - haloperoxidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HIR5163768.1"
                     /translation="GLALGKAVAAVFVARAGADGMRTAGGTPAQWQAFADSALARGEV
                     PWISQETPPRPPMLPNFGLVQTWLMTPTEVVTERPPAPPSTSSPQMQQEVAEVHRTVE
                     HLTQAQLAIAYKWNDGAGTYTPPGHWNDIAAEYVRDARLSEVRAARAFALLNMAMQDA
                     AVGCWGVKFLYFNPRPEQLDPGIKTVIGLPNFPSYPSGHSTFSSAAAVVLSYLFPAGA
                     PDFEAMKEEASISRLYGGIHYRSDIERGKEHGARIGGYAVRFAQQDGAN"
     gene            806..1396
                     /locus_tag="AC16JC_03380"
     CDS             806..1396
                     /locus_tag="AC16JC_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007071166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163769.1"
                     /translation="MASPAVATPPSHPHGVRSFFGKLNSRWHKRALQAFMIVVIAHWA
                     EHLVQAYQVYVLGWPLHQARGVLGQAFPWLVHSEVLHYGYALIMLVGIWTLLPGFVGR
                     SRTWWLAALVIQFWHHFEHALLQVQAITGRNLFGASVPTSILQLVIPRLELHLFYNTV
                     VFIPMMVAMYYHLFPSSPDAVRMQCSCALHSRPATT"
     gene            complement(1422..2465)
                     /locus_tag="AC16JC_03385"
     CDS             complement(1422..2465)
                     /locus_tag="AC16JC_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR5163770.1"
                     /translation="MLNVGLVGFGFAGKVFHAPVIGAVEGLRLTTVVQRSGAPDPRSA
                     DVAYVRSIDEMLTRSIDLVVIATPNPSHHPIAKQCLLAGCHVVIDKPFTTTVAEAEDL
                     VRVGTEQGRILSCYQNRRYVGDFVTLQALLGEGALGRVAIFESHFDRFRPALKPAAWR
                     ERPQPGSGLWFDLGAHLLDQALLLFGTPQAISADIRSERDGAAVDDAFDVTLHYPGMR
                     ALLRASMLAAAPGPTFAVHGTKGSFVKYGLDPQEAALKAGRTPAESDWDAEPSEMHGT
                     LTTPEGIRTIPTTPSSFKFFYENIRDAILGKADLAVTPAQALDVMHGLELAVVSSRTR
                     CARPWTASGQDGT"
     gene            complement(2501..>3210)
                     /locus_tag="AC16JC_03390"
     CDS             complement(2501..>3210)
                     /locus_tag="AC16JC_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007072116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GltB/FmdC/FwdC-like GXGXG domain-containing
                     protein"
                     /protein_id="HIR5163771.1"
                     /translation="AGRIVERRGGGALAPDSIRLRFHGTAGQSFGAFAVTGMCLELEG
                     EANDYVGKGLSGGTIAIRPFREAPYAAAGRRHVILGNTALYGATAGSLFASGGAGDRF
                     AVRNSGAVAVVEGVGSHCCEYMTGGVVVVLGETGPNFAAGMTNGTGYVLDAAGTLERR
                     TNLDSARVCSLDALDEELLAGLLREHATRTGSPLARALLTDWDRARTGFRKVAPREPI
                     VPVAPQTSRLAGAAGRV"
BASE COUNT          493 a         1086 c         1107 g          524 t
ORIGIN      
        1 cgggactggc gctcggcaag gccgtcgccg cggtgttcgt ggcccgtgcg ggtgccgatg
       61 gcatgcggac cgccggcggc accccagcgc agtggcaggc gttcgcggac agtgctctgg
      121 cgcgcggcga agtgccctgg atcagccagg aaacgccgcc ccgcccgccg atgctgccga
      181 atttcggcct ggtgcagaca tggctcatga ccccgacgga agtcgtgacc gagcggccac
      241 cggctccgcc gtccacctcg tccccccaga tgcagcagga agtcgccgag gtgcaccgga
      301 cggtcgagca cctcacgcaa gcgcagctgg cgatcgccta caaatggaac gacggcgcgg
      361 gcacctatac gccgccgggc cactggaacg acatcgcggc ggagtacgtt cgtgacgccc
      421 ggctgagcga ggtccgcgca gctcgcgcct ttgcgctgct caacatggcg atgcaggacg
      481 ccgcggtggg ctgctggggg gtcaagttcc tgtacttcaa cccccggccg gagcagctgg
      541 atccgggcat caagacggtg atcgggctgc cgaacttccc ctcgtatccg tcgggccact
      601 cgaccttctc gtctgctgca gcggtcgtat tatcgtactt gtttccggca ggcgctcccg
      661 acttcgaggc catgaaagag gaggcgagta tctcccggct ctatggcggg attcactatc
      721 gcagcgacat cgagcgcggc aaggagcacg gcgcccgcat cgggggctac gcggtgcgct
      781 ttgcccaaca ggacggagcg aactgatggc cagccccgcg gtcgcgaccc caccatctca
      841 tccccatggc gtccgctcgt tcttcgggaa gctcaacagt cggtggcaca agcgagcgct
      901 tcaggcgttc atgatcgtcg tcatcgccca ttgggccgag catctggtgc aggcctatca
      961 ggtgtacgta ctggggtggc cgctgcatca ggcacggggg gtcctgggtc aggcgtttcc
     1021 gtggctcgtg cactccgaag tgctgcacta cgggtatgcc ctgatcatgc tggtggggat
     1081 ctggacgttg ttgcccggct tcgtggggcg gtcgcgcaca tggtggctcg cggcgctcgt
     1141 gatccagttc tggcatcact tcgagcacgc gctgctccag gttcaggcca tcaccggccg
     1201 gaacttgttc ggggcgtccg ttcccacgag catcctgcag ctcgtgattc cgcggctcga
     1261 gctgcacctg ttctacaata ccgtggtctt catcccgatg atggtggcca tgtactacca
     1321 cctgttcccg agctcgcccg acgctgtgcg gatgcagtgc agctgcgccc tccactcgcg
     1381 cccagcgacg acctgaccac gccgcaccct gtacgcgtcc gtcacgtccc atcctggcct
     1441 gacgcggtcc acggccgggc gcagcgcgtc cggctgctga cgacggcgag ctccaacccg
     1501 tgcatgacat cgagtgcctg cgcgggcgtg accgccagat ccgccttccc gagtatggcg
     1561 tcgcggatgt tctcgtaaaa gaacttgaag ctcgacggag tggtcggaat ggtccggatg
     1621 ccctccgggg tggtcagtgt accgtgcatt tcggacggtt ccgcatccca gtccgattct
     1681 gcgggagtgc ggccggcttt cagcgccgct tcctgcggat cgagtccgta cttgacgaac
     1741 gagcccttcg tgccgtgcac ggcgaaggtc ggtcccggtg cggccgccag catggaggcc
     1801 cgcaggagcg cgcgcatccc cggatagtgc agcgtgacgt cgaacgcgtc gtctacggcg
     1861 gcgccgtcgc gctcgctgcg gatatcggcg gagatcgcct gtggagtgcc gaagaggagc
     1921 agagcctgat cgagcaggtg cgccccgagg tcgaaccaga ggccggagcc gggctgcggc
     1981 cgttcgcgcc acgcggccgg tttcagcgcg gggcggaaac ggtcaaaatg ggattcgaag
     2041 atcgcgacgc gaccgagcgc cccttccccc aggagtgcct gcagcgtcac gaaatctccg
     2101 acgtaccgtc ggttctggta gcatgacagg atgcgtcctt gctcggttcc gacccggacg
     2161 aggtcttcgg cttccgcaac ggtcgtggtg aacggcttgt cgatgacgac gtggcacccg
     2221 gccagcaggc actgcttcgc gatcggatga tgcgacggat tgggtgtggc gatgacgacc
     2281 aggtcgatgg atcgcgtgag catctcatcg atgctgcgga cgtatgcgac gtcggcagag
     2341 cgcgggtctg gtgcgccgct gcgctggacg acggtggtga gacggagacc ctcgaccgcg
     2401 ccgatgacgg gcgcgtggaa gaccttgccc gcgaagccga aaccgacgag gccgacgttc
     2461 agcatgccgc gcgcttcacc cggccgggac gctgacgact ctacaccctg cctgcggcgc
     2521 ccgcgagtcg ggaggtctgg ggagcgacgg ggacgatggg ttcgcgcggt gcgaccttgc
     2581 gaaatccggt gcgggcccgg tcccagtccg tgagcagggc gcgcgccagc ggactgccgg
     2641 tgcgggtcgc gtgctcgcgc aacaggccgg ccaggagctc ctcgtcgagc gcgtcgaggg
     2701 agcacacgcg tgcgctgtcg aggttggtgc gccgctcgag ggtccccgcc gcgtcgagga
     2761 cgtagccggt cccgttggtc atgccggccg cgaagttggg gccggtctca cccagcacca
     2821 ccaccacgcc gccggtcatg tactcgcagc agtggctccc caccccctcg accaccgcca
     2881 cggcgcccga gttgcgcacg gcgaaccggt cacccgcccc gccggaggcg aacaacgacc
     2941 ccgccgtggc gccgtagagg gccgtgttgc cgaggatgac gtggcgccgg cccgccgcgg
     3001 catacggcgc ctcgcggaaa ggacggatcg cgatcgtgcc gcccgagagg cccttcccga
     3061 cgtaatcgtt cgcctcgcct tctagctcga ggcacatgcc ggtcacggcg aacgcgccga
     3121 aactctggcc ggcggtgccg tggaaccgca gccgaatcga gtcgggcgcc agcgcgccgc
     3181 caccgcgtcg ctcgacgatc cggcccgcga
//
LOCUS       DBNGZW010000137         1781 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1308150, whole genome shotgun sequence.
ACCESSION   DBNGZW010000137 DBNGZW010000000
VERSION     DBNGZW010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1308150"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="AC16JC_03395"
     CDS             <1..1020
                     /locus_tag="AC16JC_03395"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5163772.1"
                     /translation="QLYAIGFDGKGARRLTTTAGTHQIDMSPDTRYYIDRWSNVGQPR
                     QVELWATGGKMLKRLEDNARVTQWLASHAYSAPELLTFTTSDSARIDISVVKPWPFDS
                     TRRYPMVFAIYGGPGSQQVYNSFSASAWTQWLAQQGYLVVGVNNRGTNNYGSRFMKTT
                     YKRLGYWEAHDFAETARYLASKTWADGARVAIVGTSYGGYSTVYTMEMYPDIFTVGVA
                     NSPVTDWRLYDTIYTERYMGLLSDNATGYDSSSAVTHAGQIKGHLLLIHSMMDDNVHP
                     QNTMQLLTALADARIDVDLRIYPPGHHGAAYNAQSFFLIQQATNDFLARHLKAAAGGA
                     TSSGR"
     gene            1127..>1781
                     /locus_tag="AC16JC_03400"
     CDS             1127..>1781
                     /locus_tag="AC16JC_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaD family glycine zipper/OmpA-like
                     lipoprotein"
                     /protein_id="HIR5163773.1"
                     /translation="MQINNRFASRTLPFAIVMAVGLNSCATHTQTGAVIGAAGGAVVG
                     GVIGKATGSTARGAIIGAAVGGAAGAIIGAQMDKQAKELAQNIPGATVERVGEGIAVT
                     FASGLLYDFDSDVVRAEARKNLRTLAESLDKYPGSDILIIVHTDQVGTATDNQGLSER
                     RAAAAANYLVSEGVSRTRVATRGMGETDPVASNDTEAGRAQNRRVEVAIYASEAYKAE
                     "
BASE COUNT          331 a          593 c          599 g          258 t
ORIGIN      
        1 cagctctatg cgatcgggtt cgacggcaag ggggcgcgcc ggctcacgac gaccgcgggc
       61 acgcaccaga tcgacatgtc gcccgacacc cggtactaca tcgaccggtg gtcgaacgtc
      121 ggccagcccc gacaggtcga actgtgggcg acgggcggga aaatgctcaa gaggctggaa
      181 gacaacgccc gggtcaccca gtggctcgcg tcccacgcct attcggcccc cgagctgctc
      241 accttcacga cgagcgactc ggcgcggatc gatatttcgg tcgtgaagcc gtggccgttc
      301 gactccacgc gccgctatcc catggtgttc gccatctacg gaggcccggg ctcgcaacag
      361 gtctacaact cgttcagtgc gagcgcctgg acgcagtggc tggcccagca gggctatctc
      421 gtcgtcggcg tgaacaaccg cggtaccaac aactacggca gcaggttcat gaagaccacg
      481 tacaagcgcc tcggctactg ggaggcccac gacttcgccg agaccgcccg ctatctcgca
      541 tcgaagacct gggccgacgg ggcgcgcgtc gccatcgtgg gcacgagcta cggcggttac
      601 agcaccgtgt acacgatgga gatgtacccg gacatcttca cggtcggcgt cgcgaattca
      661 cccgtcaccg actggcggct gtacgacacg atctacaccg agcgctacat ggggctcttg
      721 tccgacaacg ccacgggcta cgactcgagc tcggccgtca cgcacgcggg gcagatcaag
      781 ggccacctgc tgctgatcca ttccatgatg gacgacaacg ttcatccgca gaacaccatg
      841 caactgctca ccgcgctcgc cgacgcccgt atcgacgtcg acctgcgcat ctacccgccg
      901 gggcatcacg gggccgccta caatgcgcag agcttcttct tgatccagca ggcgaccaat
      961 gacttcctgg cgcggcatct gaaggccgcg gcgggcgggg ccacgagcag cggtcgatga
     1021 ggtgcgtggc ccgatcaccg ggccctgggt cctatcatcc gaacgagggg acgcgcggcg
     1081 gggggttgtg aggcggagat gagccagtcc cctacaggag gtgctcatgc agatcaacaa
     1141 tcgcttcgcg tcacgcacgc tgccattcgc catcgtgatg gccgtgggac tcaacagctg
     1201 cgcgacccac acgcaaaccg gtgcggtcat cggcgcggcg ggcggcgccg tcgtgggcgg
     1261 cgtgatcggg aaagccaccg gctccactgc gcgcggcgcc atcatcggcg cggcggtcgg
     1321 cggcgcggcg ggcgcgatca tcggcgcgca gatggataag caggccaagg agctggcgca
     1381 gaacatcccg ggcgccaccg tggagcgcgt cggggagggc atcgcggtga ccttcgcttc
     1441 agggctgctc tacgacttcg actccgacgt ggtccgggcg gaagcgagga agaacctgcg
     1501 gacgctggcg gagagcctcg acaagtatcc cggttcggac attcttatca tcgtccacac
     1561 cgaccaggtc ggcacggcca cggacaacca gggtctctcc gagcggcgcg ccgccgcagc
     1621 ggcgaattac ctcgtctcgg agggggtgag caggacccgc gtggcgacgc gcggcatggg
     1681 agagacggac ccggtcgcct ccaacgacac cgaggccggc cgggcacaga accgccgggt
     1741 ggaagtcgcg atttacgcga gcgaggcgta caaggcggag g
//
LOCUS       DBNGZW010000138         4651 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_77526, whole genome shotgun sequence.
ACCESSION   DBNGZW010000138 DBNGZW010000000
VERSION     DBNGZW010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4651)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4651)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4651
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_77526"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2235)
                     /gene="carB"
                     /locus_tag="AC16JC_03405"
     CDS             complement(<1..2235)
                     /gene="carB"
                     /locus_tag="AC16JC_03405"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR5163774.1"
                     /translation="MPKRTDLRRILLLGSGPIVIGQAAEFDYSGTQAVKALKEEGYEV
                     ILVNSNPATIMTDPELADRTYIEPVTPEWVRLVIERERPDALLPTMGGQTALNVATAL
                     AKNGTLAEFGVELIGASARAIQMAEDRAEFAAALRRIGLDAPRGRTVASLAEALEAVA
                     ATGYPAIIRPSFTLGGTGGGVAYNRAEFEELVEGALELSPVHSTLIERSVLGWKEYEL
                     EVMRDGRDNVVIVCSIENLDPMGVHTGDSITVAPAMTLTDREYQRMRDASIAIIREVG
                     VAAGGCNIQFAVNPADGEMLVIEMNPRVSRSSALASKATGFPIARIGAKLAVGYTLDE
                     LPNDITKTTPASFEPVLDYVVVKVPRFAFEKFPTADFRLTTQMKSVGEAMAIGRTFKE
                     AFQKGLRALEAGRPGWVVGQTLGDDRLTSDAPEDLRVALRTPTPERIFQVKRALAAGL
                     SVDAITQASGIDPWFLFQMDELLEAEHWFTGLAQVGAAELRRMKRMGFSDCQLATLRG
                     TTEEAVREQRWALGVRPAYKTVDTCAGEFPSSTPYLYSSYDDENESEAFGDRGIVILG
                     SGPNRIGQGVEFDYCCVRAGLAFRDLGFKTIMINSNPETVSTDFDISDKLYFEPLTFE
                     DVIEIVSLERPRGVVVQLGGQTPLRLTNALAAAGVPILGTSPDAIDTAEDRERFEQLA
                     RRLGVTQPPNGTARSVAEALAVADRIGYPVLIRPSYVLGGRAMEIVYDPGSLREFFEK
                     AARVA"
     gene            complement(2219..3169)
                     /locus_tag="AC16JC_03410"
     CDS             complement(2219..3169)
                     /locus_tag="AC16JC_03410"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5163775.1"
                     /translation="MKVLVTGADGFVGSWLVRRLVADGHAVTGTFRLEEDQRAKRYDA
                     ALRDRLSWVPLELADEESVRRAVEGDWDAVVHLAAVSWVTVAERDPGRAWNENAGGTA
                     RLARALADMKRAGDADPLLLVVSSVEVYGPGTEAPRRETDPVAPVSAYAASKVGAEVA
                     ALSEWRRTGLRVVVARPSPHTGHGQSERALVPRYAHRVLTARRARAPAIATGLLDGVR
                     DILHVADVVDAYMRLLGAGVPGEVYNVSSGNGVALKDLVVRLCALAGWQPILEVDAAD
                     VRPDAVPHLVGDGARLRALGWVPRRSLDETLQDVLDAQAD"
     gene            complement(3166..4209)
                     /gene="gmd"
                     /locus_tag="AC16JC_03415"
     CDS             complement(3166..4209)
                     /gene="gmd"
                     /locus_tag="AC16JC_03415"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019673 - GDP-mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HIR5163776.1"
                     /translation="MSPSARSPGRVRGGEKRGKPKPATKAEQPRRRALITGVTGQDGS
                     YLAELLLGKGYEVVGVVRRTSHDSYERIGHLLGRLQVVPADLLDQHSLTAVVRDAKPH
                     EVYNLAAQSFVPTSWTQPVLTGEFTALGVTRLLEAVRLAHPEARFYQASSSEMFGKAV
                     ETPQRESTPFYPRSPYGVAKCYGHWITVNYRESYGLYAVSGILFNHESPRRGLEFVTR
                     KVSHSVARIAKGLDRELRLGNLDASRDWGYAGDYVDAMWRMLQQPAPDDYVIGTGESH
                     TVRDLVAIAFEHVGLDWKKHVVVDRALVRPAEVDVLRADPSKARRKLDWRPTVGFAEL
                     VRMMVAADLERLE"
     gene            4225..4416
                     /gene="rpmB"
                     /locus_tag="AC16JC_03420"
     CDS             4225..4416
                     /gene="rpmB"
                     /locus_tag="AC16JC_03420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR5163777.1"
                     /translation="MARVCEICGKGPTTGHNVSHANNKTNRRWYPNLQRVRAVVDGRT
                     RRIRACTRCIKSNKVTKAA"
     gene            complement(4475..4639)
                     /locus_tag="AC16JC_03425"
     CDS             complement(4475..4639)
                     /locus_tag="AC16JC_03425"
                     /inference="COORDINATES: protein motif:HMM:NF013369.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL17 family ribosomal protein"
                     /protein_id="HIR5163778.1"
                     /translation="MVERRIKDREVTHRLFAELGKRFAARPGGYTRIVKLGHRPGDGA
                     DVARIELLSE"
BASE COUNT          706 a         1712 c         1553 g          680 t
ORIGIN      
        1 cgccacccgg gcggccttct cgaagaactc ccgcaacgac cccgggtcgt acacgatctc
       61 catcgcccgt ccgcccagga cgtaggaggg tcggatgagc accgggtagc cgatgcggtc
      121 ggcaaccgcg agcgcttccg cgaccgagcg cgccgtgccg ttgggtggct gcgtcacccc
      181 caggcggcgc gccagctgct cgaagcgctc ccgatcctcg gccgtatcga tcgcatcggg
      241 gctcgtcccc aggatgggca cgccggctgc agcgagggca ttggtgaggc gcaacggagt
      301 ctggcccccg agctggacga cgacgccgcg gggacgctcg aggctgacga tctcgatgac
      361 gtcctcgaac gtgagcggct cgaagtacag cttgtccgag atgtcgaagt cggtggagac
      421 cgtctcgggg ttcgagttga tcatgatcgt cttgaacccg aggtcgcgga acgcgagccc
      481 ggcgcgcaca cagcagtagt cgaactccac tccctgcccg atgcggttcg gcccgctccc
      541 gaggatcacg atgccccggt cgccgaacgc ctccgattcg ttctcgtcat cgtaggagga
      601 gtagaggtag ggcgtagacg acgggaactc ccccgcgcag gtgtccaccg tcttgtaggc
      661 gggccggacg cccagggccc agcgctgctc gcgcaccgcc tcctcggtgg tgccgcgcaa
      721 cgtcgccagc tggcaatcgg agaaacccat ccgcttcatg cgacgcaact ccgccgcgcc
      781 cacctgcgcc aatccggtga accagtgctc ggcctcgagc agctcgtcca tctggaacag
      841 gaaccagggg tcgatgccgg aggcctgcgt gatggcgtcc accgagagcc cggccgcgag
      901 cgcccgcttg acctggaaga ttcgctcggg cgtgggggtc cgcagtgcca cccgcaggtc
      961 ctccggcgcg tcgctcgtca accggtcgtc gcccagcgtc tgccccacca cccaccccgg
     1021 gcgtcccgcc tcgagcgcgc gcagcccctt ctggaacgct tccttgaagg tgcggccgat
     1081 ggccatcgcc tcgcccaccg acttcatctg cgtcgtcagc cggaagtcgg cggtcgggaa
     1141 cttctcgaac gcgaagcgcg gcaccttcac gacgacgtag tccagcacgg gctcgaagga
     1201 ggccggtgtg gtcttcgtga tgtcgttggg cagctcgtcg agcgtatacc cgacggcgag
     1261 cttcgccccg atgcgggcga tggggaatcc ggtggccttg gacgcgagcg ccgaggagcg
     1321 cgacacgcgg ggattcatct cgatcaccag catctcgccg tcggccgggt tcacggcgaa
     1381 ctggatgttg cagccgcccg cggccacgcc gacctcgcga atgatggcga tcgacgcatc
     1441 gcgcatccgc tgatactcgc ggtcggtgag cgtcatggcg ggcgccacgg tgatcgaatc
     1501 gccggtgtga acgcccatcg gatcgaggtt ctcgatcgag cagacgatga cgacgttgtc
     1561 gcggccgtcg cgcatcacct cgagctcgta ttccttccag cccagcacgg aacgctcgat
     1621 cagggtcgag tgcacgggcg acagctccag cgcgccctcg accagctcct cgaactccgc
     1681 ccggttgtac gcgacgccgc ccccggtgcc gcccagtgtg aacgacggcc ggatgatcgc
     1741 tggatacccc gtcgccgcca ccgcctcgag cgcctccgcc aacgacgcca ccgtgcggcc
     1801 gcgcggtgcg tccagcccga tgcgccgcag cgccgccgcg aactcggccc ggtcttccgc
     1861 catctggatc gcgcgcgccg aggcgccgat cagctcgacc ccgaactccg ccagcgtgcc
     1921 gttcttggcc agcgcggtgg cgacgttcaa cgccgtctgg ccgcccatgg tcggcagcag
     1981 cgcgtcgggg cgctcgcgct cgatcacgag acggacccac tccggcgtga ccggctcgat
     2041 gtaggtgcgg tcggccagct cgggatcggt catgatggtg gcggggttcg agttcacgag
     2101 aatgacctcg tacccttctt ccttgagcgc cttcaccgcc tgcgtgcccg aataatcgaa
     2161 ctcggcggcc tgaccgatca cgatcgggcc ggaccccagc agcaggatgc ggcgcaggtc
     2221 agtccgcttg ggcatcgagc acgtcctgca gcgtttcgtc cagagaccgg cgcggcaccc
     2281 aacccagcgc gcgcagcctg gcgccgtcgc ccacgagatg cggcacggcg tcggggcgga
     2341 cgtccgcggc gtccacctcg agaatcggct gccagcccgc gagcgcgcag agccgcacca
     2401 ccaggtcctt cagtgccacg ccgttgcccg atgacacgtt gtagacctcg ccgggaacgc
     2461 ccgcgcccag cagccgcatg taggcatcga ccacgtccgc cacgtgcagg atgtcgcgca
     2521 cgccgtccag cagaccggtg gcgatcgccg gggcgcgggc gcgccgcgcc gtgagcacgc
     2581 ggtgcgcgta gcgcggcacc agggcccgct cgctctggcc gtggcccgta tgcggcgagg
     2641 gccgggccac caccacccgc aaccccgtgc ggcgccactc cgacagcgcc gcgacttcgg
     2701 cgccgacctt gctcgccgcg taggccgaca cgggcgccac gggatccgtc tcgcgccgcg
     2761 gcgcctccgt gcccggcccg tacacctcga cgctcgagac cacgagcagg agcggatccg
     2821 cgtcccccgc ccgcttcata tcggcgagcg cgcgggcgag ccgggcggtc ccgcccgcgt
     2881 tctcgttcca ggcccgcccc ggatcgcgct ccgccacggt gacccagctg acggccgcga
     2941 gatggaccac ggcgtcccag tcgccttcga ccgcccgccg caccgactcc tcgtcggcca
     3001 gctcgagcgg cacccagctg agccggtcgc gcagcgcggc gtcgtagcgc ttcgcccgct
     3061 ggtcctcctc gagccggaag gtccccgtca ccgcatgccc gtcggcgacg agccggcgca
     3121 cgagccacga tccgacgaaa ccatcggctc cggtgacgag caccttcact cgagtcgctc
     3181 caggtcggcc gcgaccatca tgcgcaccag ctccgcgaac ccgaccgtgg ggcgccagtc
     3241 cagcttgcgc cgggccttcg acggatcggc gcgcagcacg tccacctcgg ccggccgcac
     3301 cagcgcgcgg tccaccacga catgcttctt ccagtccagc cccacgtgct cgaaggcgat
     3361 cgcgaccagg tcccgcaccg tatgcgactc gccggtcccg atgacgtagt cgtcgggcgc
     3421 cggctgctgc agcatgcgcc acatcgcgtc gacatagtct ccggcataac cccagtcgcg
     3481 gctcgcgtcc agattgccga gccgcagctc ccggtccagc cctttcgcga tgcgggccac
     3541 gctgtggctc accttgcggg tgacgaactc gaggccccgg cggggcgatt cgtggttgaa
     3601 caggatgcca ctcacggcgt acaggccgta ggactcccgg tagttcaccg tgatccagtg
     3661 cccgtagcac ttggccacgc catagggcga gcgggggtag aacggcgtgg attcgcgttg
     3721 cggcgtttcc accgccttgc cgaacatctc cgaggagctg gcctggtaga accgcgcctc
     3781 gggatgggcg aggcggacgg cctccagcaa ccgggtcacg cccagggcgg tgaactcgcc
     3841 cgtgaggacg ggctgcgtcc aggacgtcgg gacgaaactc tgggcggcca ggttgtagac
     3901 ctcgtgcggc ttggcgtcgc gaaccaccgc ggtgagggaa tgctgatcga gcagatcggc
     3961 cggcacgacc tggaggcggc cgagcaggtg tccgatgcgc tcgtaggagt cgtgcgaggt
     4021 ccggcggacc acgccgacga cctcgtaccc cttgccgagc agcagctccg ccagatagga
     4081 cccgtcctgg ccggtgaccc ccgtaatcag ggcgcgccgc cggggttgct ccgccttcgt
     4141 cgccggctta ggtttcccgc gcttttcccc cccgcggacg cggcccgggg agcgtgcaga
     4201 tgggctcatc tacgggacga ccctatggct cgagtatgcg agatttgcgg caaaggcccg
     4261 acgacgggcc acaacgtgag ccacgccaac aacaagacga accggcgctg gtatcctaac
     4321 ctgcagcgtg tgcgcgcggt ggtggacggc cgcacgcggc gtatccgcgc ctgcacccgg
     4381 tgcatcaagt cgaacaaggt gaccaaggcc gcttgaaaga agggggaccc atcgggcccc
     4441 ccttcgcgca ttcgcttccc tccgcgccag tcgctcactc actcagcaac tcgattcgcg
     4501 ccacgtccgc gccgtcgccc ggccggtggc cgagcttgac gatgcgcgtg tagccgccgg
     4561 ggcgggccgc gaagcgcttc cccagctcgg cgaagagccg gtgggtgacc tcgcgatcct
     4621 tgatgcggcg ctcgaccagg cggcgtgcgt g
//
LOCUS       DBNGZW010000139         2747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1444560, whole genome shotgun sequence.
ACCESSION   DBNGZW010000139 DBNGZW010000000
VERSION     DBNGZW010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1444560"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            128..847
                     /locus_tag="AC16JC_03430"
     CDS             128..847
                     /locus_tag="AC16JC_03430"
                     /inference="COORDINATES: protein motif:HMM:NF014671.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR5163779.1"
                     /translation="MTADSALVEGWPVWLEVNGEPVVTWMCTPDQLEELAAGWLHGEG
                     YIESVDDLLKLRPCATDLGFWADVRPEGLVRVKAENRRRVLASGCGAVTTLLADPHAV
                     PRAVVRGEPPTAEQLRTLFKAVFARGARYNETGGIHAAALTDAATLLFHAEDIGRHNA
                     VDKVIGAAVLARRPIAGLGLLVTGRISAELAFKASRAGLAWVATPSVPSTLALVIAQR
                     SGMVLVGRAVSGSPHVHRPDA"
     gene            844..1287
                     /locus_tag="AC16JC_03435"
     CDS             844..1287
                     /locus_tag="AC16JC_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR5163780.1"
                     /translation="MTSEALRPLLRDARTIAVVGLSPDPARPSHSVGRYLQGAGFRIV
                     PVNPHHTHVLGERCYPSLAAAARDHSLDVVDVFRRSAFAGAVVDEALALRPPPRLIWL
                     QQGVVDPAAAERARSAGVPFVMDRCLAVDHELLFFDADRHHLEAR"
     gene            1284..2099
                     /locus_tag="AC16JC_03440"
     CDS             1284..2099
                     /locus_tag="AC16JC_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163781.1"
                     /translation="MSRLTFALILTCAGQITAAAQERPSSPTLEFTIHGGVAGGHSLW
                     GISKQPICNTGVGGTCTGASYDTVRIARTVGSGITAGLSVAYYPGAHIGYYGEASFVG
                     LSLDDACSGIYFAGGSGSVHDLLCQAVAGSSTTLGAASFIGGITVRGAPGAGVSPYVV
                     LGGGLLVFSRSTLDMSGDVVTGGGVQDLSIVVDNSPHRNSPLLAVGAGLRMRAGQGYG
                     VRVAFEDLIARFQRLTGPVNAIGQGPVASKFYHHFVVHLGFDVVLEKSRGRRY"
     gene            2101..2700
                     /gene="mobA"
                     /locus_tag="AC16JC_03445"
     CDS             2101..2700
                     /gene="mobA"
                     /locus_tag="AC16JC_03445"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HIR5163782.1"
                     /translation="MRGAVLAGGAASRYGGSPKGLLAVGGRRILDRVVDAITAAVGAP
                     PLLVANDPDAGSWAPALRCIPDVRPGAGSLGGIYTAVCDAPGPVLCVAWDMPFVSAGL
                     LRTLVAGSGGFDAFLPESGGRRGLEPLCAVYGPACRAPIEGAMARGDLRAIAFHDAVR
                     VGTLSLDRVRSFGDPETLFFNVNRPEDMEQAEVLWRRRA"
BASE COUNT          352 a          964 c          996 g          435 t
ORIGIN      
        1 gccggcgcgt tgccgcccgg cgtgcagctc catgcgatcc gcgtccagga gggaccccat
       61 ctctactccg agtacttcgg cgagccctga acgcgaagtg cccgcccccg ggtcgcgccg
      121 cggacggatg accgcggact ccgccctcgt cgaagggtgg ccggtctggc tcgaggtgaa
      181 cggcgagcct gtcgtgacgt ggatgtgcac gccggaccag ctcgaagagc tggcggccgg
      241 ctggctgcac ggcgagggct acatcgaatc ggtggacgac ctgctcaagc tgcggccctg
      301 cgccaccgac ctgggcttct gggcggacgt gcggcccgag gggctggtcc gcgtgaaagc
      361 ggagaatcgc cggcgcgtgc tcgcgtccgg atgcggcgcc gtcacgacgc tgctcgccga
      421 tccgcacgcg gtgccgcgcg ccgtggtgcg cggcgagcca cccaccgccg agcagctgcg
      481 cacgctgttc aaggcggtgt tcgcccgcgg cgcgcgctac aacgagaccg gaggcatcca
      541 cgccgccgcg ctgaccgacg ccgccacgct gctgtttcac gccgaggaca tcgggcggca
      601 caacgcagtg gacaaggtga tcggcgcggc cgtgctggcg cgccgcccga tcgccgggct
      661 cgggctgctc gtgacggggc gcatctccgc ggagctggcg ttcaaggcgt cgcgcgcggg
      721 tctcgcctgg gtggcgacgc cgagcgtccc gtccacgctg gcgctggtga tcgcgcaacg
      781 ctcggggatg gtgctcgtgg gacgcgcggt gagcggatcg ccgcacgtcc accggcccga
      841 cgcgtgacct ccgaggcgct gcgccccctg ttgcgcgatg cccgcacgat cgcggtcgtg
      901 ggcctgtcgc ccgatccggc ccggcccagc cactcggtgg gccgctacct gcagggcgcg
      961 ggattccgca tcgtgccggt gaatccccac cacacgcatg tgctgggcga gcgctgctac
     1021 ccgtccctcg cggcggcggc gcgcgaccat tcgctcgacg tcgtcgatgt gttccggcgc
     1081 agcgcgttcg cgggcgccgt cgtggacgag gcgctggcgc tgcgaccccc accccggctc
     1141 atctggctgc agcagggcgt cgtcgatccc gccgcggcgg agcgggcacg gagcgcgggg
     1201 gtgccgttcg tcatggatcg ctgcctggcc gtggaccatg aactcctgtt cttcgacgcc
     1261 gaccgccatc acctggaggc gagatgagtc gattgacgtt cgctctgatt ctgacgtgtg
     1321 cgggacagat cacagccgcc gcccaggagc gcccctcgtc gcccacattg gagttcacga
     1381 ttcacggcgg cgtggccggc gggcactccc tgtgggggat ctccaaacaa cctatctgca
     1441 acacaggtgt cggcggaacg tgcaccggag cctcatacga cacggtgcgg atcgcccgca
     1501 ccgtgggaag tggcatcacg gccgggctct cggtcgccta ctacccggga gcgcacatcg
     1561 gctattacgg cgaggcctcc tttgtcggct tgtcactgga cgacgcctgt tccggcatct
     1621 atttcgcggg aggctcggga agcgtgcacg atctcctgtg tcaggcggtt gccgggtcct
     1681 cgaccaccct cggtgcggca tcctttatcg ggggcatcac ggtgcggggc gcgccgggcg
     1741 cgggcgtcag cccctacgtc gtcctcggcg gtggactgct cgtcttctcg cggagcacgc
     1801 tcgatatgtc gggagatgtg gtcacgggag gtggcgtgca ggacctttcc atcgtcgtgg
     1861 acaactcccc gcaccgcaat tcccccctgc tcgccgtggg agccggattg cggatgcgcg
     1921 ccggccaggg ctatggcgtg cgagtggcgt tcgaggacct gatcgcaagg ttccagcgcc
     1981 tcaccgggcc ggtgaacgcg atcggccagg gaccggtcgc gtcgaagttc taccaccact
     2041 tcgtcgtgca cctgggtttc gacgtcgtgc tcgagaagag ccgcggccgg cgctactagc
     2101 gtgcgcggcg ccgtgctcgc cggcggagcc gcgagccgct acggcggcag cccgaagggg
     2161 ttgctcgcgg tgggtggccg ccgcattctg gaccgcgtgg tggacgcgat cacggcggcc
     2221 gtgggcgctc ccccgctgct cgtcgcgaac gacccggacg ccggctcctg ggcaccggcc
     2281 ctgcgctgca tccccgacgt gcgcccgggc gcggggagcc tgggcggcat ctacaccgcc
     2341 gtgtgcgacg cacccggccc ggtgctgtgc gtcgcctggg acatgccgtt cgtgagcgca
     2401 ggcctgctcc ggaccctcgt ggccggcagc ggcgggttcg acgcctttct ccccgagagc
     2461 ggggggcggc gcggcctcga gccgttgtgc gcggtgtacg gccccgcctg ccgcgcgccg
     2521 atcgaggggg ccatggcccg cggcgacttg cgcgccatcg ccttccacga tgccgtgcgc
     2581 gtgggtacac tatcgctcga ccgggtccgc tcgttcggcg atcccgagac cctgttcttc
     2641 aacgtcaatc gcccagaaga catggagcag gcggaggtgt tgtggcggcg acgcgcataa
     2701 tctccatcat cgggaagaag gacgcgggca agacctcgct cgtggtg
//
LOCUS       DBNGZW010000140         3232 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1173154, whole genome shotgun sequence.
ACCESSION   DBNGZW010000140 DBNGZW010000000
VERSION     DBNGZW010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3232)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3232)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3232
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1173154"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..725)
                     /locus_tag="AC16JC_03450"
     CDS             complement(12..725)
                     /locus_tag="AC16JC_03450"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0140098 - catalytic activity, acting on
                     RNA [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HIR5163783.1"
                     /translation="MRLQRALARAGVTSRRKAEDLIRAGRVRVDGAVATIGTSVDPDR
                     QRITVDGTAVRPATGPATWLALNKPLGYVVSRGDPAGRPTVFDLVPVVRGLTYVGRLD
                     VMTSGLLLLTTDGAAAHRLMHPRFAVERTYMLRAHGRPAEEIRAALARPITIDGRRVA
                     VRRFRVRPVARSIELELTLAEGRQRIVRRLAEALGLKVEWLHRTRYGPVRLGDLAEGR
                     HRPLTRREIAAIEEPDGAA"
     gene            complement(731..1279)
                     /gene="scpB"
                     /locus_tag="AC16JC_03455"
     CDS             complement(731..1279)
                     /gene="scpB"
                     /locus_tag="AC16JC_03455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR5163784.1"
                     /translation="MSPLAKLLEAALFAASRPLGLDELATLDPEAGEAGTRSALAELR
                     DTFVAADHGVELVEIAEGWQFLTRPEYAEPIERAQFALRPRRLSPAALETLAIIAYRQ
                     PVGRVEVDEIRGVDSGAVIDKLLERGLIEITSRGEGLGRPLLYSTTPQFLEILGLKGL
                     EGLPRLDELSVSLRPPAPAQPE"
     gene            complement(1254..2006)
                     /locus_tag="AC16JC_03460"
     CDS             complement(1254..2006)
                     /locus_tag="AC16JC_03460"
                     /inference="COORDINATES: protein motif:HMM:NF014654.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR5163785.1"
                     /translation="MTAVPPPPSSAGAAAGGGFTVELEQFSGPLDLLLHLIREDEVDI
                     TDLPIARIADQFLAAINELGLNQAADYLEMAARLLRIKAQLLLPRRLGEDEWEDPRSE
                     LVRRLLEYQQIRELVDWLAGAAAIRAERFGRAFEQPGPDLPPAPLAIELNELLAAAER
                     VVALIPEPTLHRVVPRPLDVEGATARILALLAERDTLQWHDVVSERAGVVEVLSAFIA
                     LLELAKRSAVRLDQPEPFAPMVIRRESPREAA"
     gene            complement(2003..2638)
                     /locus_tag="AC16JC_03465"
     CDS             complement(2003..2638)
                     /locus_tag="AC16JC_03465"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR5163786.1"
                     /translation="MVAHEYAHAEAAYRQGDDTAYMLGRLTMNPLKHIDPFMTVLLPI
                     LLWVGSQGRYTFGAAKPVPVNPRKYRRYVRGDVIVSLAGIATNLALFAVFTGAFALVG
                     MLGRSLPPLAATMGLLQRMVVFGMWFNLILAFFNLIPIPPLDGSHAFYHILPPAAGAR
                     YRALGRFGFLPIMALTYFLPGLFQIILWPAGQLMQLGIAMVGGYRLPGVTQ"
     gene            2810..3064
                     /gene="rpsT"
                     /locus_tag="AC16JC_03470"
     CDS             2810..3064
                     /gene="rpsT"
                     /locus_tag="AC16JC_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013627025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HIR5163787.1"
                     /translation="MPRIKSAKKAMRQARRHTIVNRAQRSALRTALKRVRSAGTKQEA
                     ASAYAATVRLLDRAARKGLIHKNNAARNKSRLAKFVETKA"
BASE COUNT          529 a         1164 c         1107 g          432 t
ORIGIN      
        1 cgaccgtcgc gtcacgcagc tccatccggc tcctcgatgg ccgcgatttc gcggcgggtg
       61 agcggccggt gccgtccttc ggcgagatcc ccgagccgga ccggtccgta ccgcgtgcgg
      121 tgcaaccact ccaccttcag ccccagcgct tccgcgagcc gccgcacgat gcgctgccgc
      181 ccctcggcga gcgtcagctc cagctcgatg ctgcgcgcca ccggtcgcac ccggaagcgg
      241 cgcacggcga cgcgccggcc gtcgatggtg atcggccggg ccagcgccgc gcggatctcc
      301 tcggccggcc ggccgtgcgc gcggagcatg taggtccgct ccaccgcgaa ccgcgggtgc
      361 atcaggcggt gcgcggcggc gccatcggtc gtgagcagga gcaggccgga cgtcatcacg
      421 tccagccgcc ccacatacgt gagcccccgg accaccggga cgagatcgaa gaccgtggga
      481 cgaccggccg gatccccccg gctcacgaca tagccgagcg gcttgttcag cgcgagccac
      541 gtcgccggtc cggtcgcggg ccggaccgcc gtgccgtcca ccgtgatgcg ctggcggtcc
      601 ggatcgaccg atgtgccgat cgtggcgacc gccccgtcca cccggacgcg cccggcccgg
      661 atcaggtcct cggccttgcg ccgcgacgtg acgcccgcgc gcgccagcgc gcgctgcagg
      721 cgcatcgggg tcattcgggc tgggccggcg ccggcggacg caagctcacc gacagctcgt
      781 ccaggcgcgg gagcccctcc agtcccttca ggcccaggat ctcgagaaac tgcggcgtcg
      841 tggagtagag caacggccga cccaatccct cgccgcggct cgtaatctcg atcagccccc
      901 gctccaacag cttgtcgatg accgcgccgg aatccacgcc gcggatttca tcgacctcca
      961 cgcggcccac cggctggcga tacgcgatga tggccagcgt ttccagcgcc gccggactga
     1021 gccggcgcgg ccgcaacgcg aactgggccc gctcgatcgg ctcggcgtat tcggggcgcg
     1081 tgaggaactg ccatccttcc gcgatctcga ccagctcgac tccatgatcc gccgcgacga
     1141 acgtgtcgcg cagctcggcg agcgcggacc gggtccccgc ttcgcccgct tccgggtcca
     1201 gcgtggccag ctcgtccaga ccgagcgggc gcgacgcggc gaacagcgcc gcctcaagca
     1261 gcttcgcgag gggactcacg gcggatcacc atcggggcga acggctcggg ttggtcgagc
     1321 ctcacggcgg agcgcttggc cagctccagc aaggcgatga acgcggacag cacctcgacg
     1381 acgccggccc gctcgctcac gacgtcgtgc cactgcagcg tgtcacgctc ggcgagcaac
     1441 gccagaatgc gggccgtcgc gccttccacg tccagcggac gggggaccac acggtgcagg
     1501 gtcggctcgg gaatcagcgc gacgacgcgc tcggcagccg ccagcagctc gttgagctcg
     1561 atggcgagcg gcgccggcgg cagatccgga ccgggctgct cgaaggcgcg tccgaaccgt
     1621 tccgcccgta tcgcggccgc gccggcgagc cagtccacca gctcccggat ctgctggtat
     1681 tcgagcagcc gccgcaccag ctccgagcgg ggatcctccc actcgtcttc gcccagcctc
     1741 cgcggcagca gcagctgcgc cttgatgcgc agcagccgcg ccgccatctc gaggtagtcg
     1801 gccgcctgat tcaatcccag ctcgttgatc gccgccagga actgatcggc gatgcgggcg
     1861 atgggcaggt cggtgatgtc gacctcgtcc tcgcggatca ggtgcagcag caggtcgagc
     1921 gggcccgaga actgctcgag ctcgaccgtg aacccgccac ccgcagcggc tccggcggac
     1981 gacggcggcg gtggcaccgc ggtcactggg tcacccccgg caggcggtag ccgcccacca
     2041 tggcgatgcc gagctgcatc agctgaccgg cgggccacag gatgatctgg aacaggccgg
     2101 gcaggaagta ggtgagcgcc atgatcggga ggaagccgaa gcgccccaac gcccgatacc
     2161 gcgcaccggc ggccggcggc aggatgtgat agaacgcatg gctgccgtcg agcgggggga
     2221 tgggaatgag attgaagaac gccaggatca ggttgaacca catgccgaac acgaccatcc
     2281 gctgcaacag gcccatcgtg gcggccagcg gcggcaggct gcggcccagc atgccgacca
     2341 gcgcgaacgc tccggtgaac acggcgaaca gcgcgaggtt ggtcgcgatc ccggcgagcg
     2401 atacgatcac gtcgccgcgc acgtaccgcc ggtacttgcg cggattgacc ggcaccggct
     2461 tcgccgcgcc gaacgtgtac cgtccctgtg agcccaccca cagcaggatc ggcagcagca
     2521 cggtcatgaa ggggtcgatg tgtttcaggg ggttcatcgt cagccgcccg agcatgtagg
     2581 cggtgtcatc gccctgtcgg taggcagcct cggcgtgggc gtattcgtga gccaccatgg
     2641 agaacagcag taccggggcc cacagcagaa catcgagtga gatagcgggg ctcggccgtt
     2701 gtggtcggcc taagataccg ccggggccgt acccgggtca acgttgtagc gccttgacca
     2761 tgctcaactt accgtctaac ttgcgcgcct cgaacgggag attccaccag tgccgcgaat
     2821 caagtccgcc aagaaggcga tgcgccaggc gcgccgccat acgatcgtca accgcgcgca
     2881 gcgctccgcc ctgcggaccg ccctgaaacg ggtccggagc gcgggaacga agcaggaggc
     2941 cgcgagcgcg tatgcggcga ccgtccgcct gctcgatcgg gctgcgcgga agggactgat
     3001 ccacaagaac aacgcggcac gcaacaagtc ccggctggcg aagttcgtgg agacgaaggc
     3061 gtaaggcagt cctacagcgc agccagctcc gccccgcagc tctcgcagta ccactccgtc
     3121 ggcaaattca tcgtgccgca ctcgcggcac ttcagcgtcc ggaccggctc cggctcatcg
     3181 ttcacgggag ccggattcgc gggcgccggc cggttgttgg ccggctcgga tg
//
LOCUS       DBNGZW010000141         3187 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1444775, whole genome shotgun sequence.
ACCESSION   DBNGZW010000141 DBNGZW010000000
VERSION     DBNGZW010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3187)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3187)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3187
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1444775"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1595)
                     /locus_tag="AC16JC_03475"
     CDS             complement(<1..1595)
                     /locus_tag="AC16JC_03475"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5163788.1"
                     /translation="MVDVPGSQLAEALRDRYALERELGRGGMATVYLAHDVKHDRPVA
                     LKVLHPELASVLGPERFQREIRLAARLQHPHILTVLDSGETAGRLWFTMPFVEGESLR
                     DRLTRERQLPVDDALRIAREAAQGLQYAHEHGVMHRDINPENILLTRDGSTLVADFGI
                     ARAFGGGEARLTETGMAIGTPAYMSPEQASGDQTLDARTDVYSLGCVLFEMLAGEPPF
                     TGPTVQAIIAKRLTQPVPSVRQSRPTVPQAAERALRQALAPIPADRYATAGDLARALQ
                     TSATPRLSAPSQSGRRRLPVAALMLVLGFLIGLGVLFAWRRSHPGGGDGAGPKRIAVL
                     PFENLGDSTDAYFADGMTDEVRGKLSQLSGLAVIARSSSNEYRRSDKSPQEIARELGA
                     EYLLTATVRWEKTPGAPSRVRVSPELVQVEPGAAPTTRWQQGFDASLTDVFQVQGDIA
                     GKVATALDVALGDSARQQLEARPTANLAAYDAFLKGEAASQVMSVGDPASLRRAIGYY
                     EQAVALDSAFGSAWTQLARARSIL"
     gene            complement(1691..2557)
                     /locus_tag="AC16JC_03480"
     CDS             complement(1691..2557)
                     /locus_tag="AC16JC_03480"
                     /inference="COORDINATES: protein motif:HMM:NF015948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR5163789.1"
                     /translation="MVRTSDRRKGVTEALRLFEPAGIAGRRVVLKPNLNSADPAPAST
                     HPDTLGQLVTELYERGARTVTLGESSGPSGTRRVMEQYGVLDLAAALRFDVVNLDELP
                     DADWIPIGVGGTHWPEGYHLPRLVTEAEYLVSTCCLKTHQYGGVFTMSLKLAVGLTPR
                     PLRRSMHRSPDMRRMIAELNAGYRPQLVVLDGVSAFTDGGPSSGERKDGNVVIVGDDR
                     VAVDAVGLAVLKDLGANDAIMSRGIWAQEQLVRAVELNLGARGPGDIELVTGDAASRA
                     YATRLERILAAG"
     gene            2940..>3187
                     /locus_tag="AC16JC_03485"
     CDS             2940..>3187
                     /locus_tag="AC16JC_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HIR5163790.1"
                     /translation="MTAPNARERILPRLIEDELKESFLDYSMSVIVQRALPDVRDGLK
                     PVHRRILYAMNELGLLSGRPYKKSATVVGDVLGKYHPHG"
BASE COUNT          495 a         1112 c         1111 g          469 t
ORIGIN      
        1 aggatcgagc gcgcccgggc cagctgggtc cacgccgaac cgaacgccga gtcgagggcg
       61 accgcctgct cgtagtagcc gatggcgcgc cgcaggctgg cggggtcacc gacggacatg
      121 acctgcgagg cggcctcacc tttcaagaag gcgtcgtacg ccgccaggtt cgcagtgggc
      181 cgggcttcga gctgctggcg cgcgctgtcg cccagcgcca cgtcgagcgc ggtcgcgacc
      241 ttgcccgcga tgtcgccctg gacctgaaac acatcggtga gcgatgcgtc gaatccctgc
      301 tgccacctgg tcgtcggcgc cgcgcccggc tcgacctgga ccagctccgg gctcacccgg
      361 acgcggctcg gtgcacccgg ggtcttctcc cagcgcaccg tcgccgtgag caggtactcg
      421 gcgccgagct cccgcgcgat ctcctgcggc gacttgtcgc tgcgccggta ttcgttggag
      481 ctggaccggg cgatgaccgc gagcccggac agctgcgaga gcttcccccg cacttcgtcg
      541 gtcatgccgt cggcgaagta ggcgtcggtc gaatccccca ggttctcgaa cggcagcacc
      601 gcgatccgct tggggccggc gccgtcgccc ccacccgggt gcgagcgccg ccaggcgaag
      661 agcaccccca ggccgatcag gaagcccagc accagcatca gcgcggccac cggcagtcgt
      721 cgccgcccgc tttgtgaggg agccgacagc cggggggtcg cgctcgtctg cagcgcgcgc
      781 gccaggtcgc ccgcggtggc atagcggtcg gccgggatgg gcgcgagcgc ctgccgtagc
      841 gcccgctctg cggcctgagg taccgtcgga cgggactgcc gcacgctcgg caccggctgc
      901 gtgagccgtt tggcgatgat ggcttgcacg gtgggcccgg tgaacggcgg ctcgcccgcg
      961 agcatctcga acagcacaca gcccagcgag tagacgtcgg tgcgggcgtc gagggtctgg
     1021 tcgccgctcg cctgctcggg gctcatgtac gccggcgtgc cgatcgccat ccccgtctcg
     1081 gtcaggcgcg cttcaccgcc gccgaatgcc cgggcgatcc cgaaatccgc caccagcgtc
     1141 gagccgtccc gggtgagcag gatgttctcg gggttgatgt cgcgatgcat gacgccgtgc
     1201 tcgtgtgcgt actgcagccc ttgggcggcc tcgcgcgcga tccggagcgc gtcgtccacc
     1261 gggagctggc gctcccgggt cagccgatcc cgtagcgact cgccttccac gaacggcatg
     1321 gtgaaccaca gccggccggc tgtctcgccg gagtcgagca cggtcagaat gtgcgggtgc
     1381 tggagccggg cggcgagccg gatctcgcgc tggaagcgct cgggccccag gaccgatgcc
     1441 agctcgggat gcagcacctt cagggccacc ggccggtcgt gcttcacatc atgagcgaga
     1501 tagaccgtgg ccatcccgcc gcgcccgagc tcgcgctcca gcgcgtagcg gtcgcgcagc
     1561 gcctcagcga gctgggagcc cggaacgtcg accacggcgg cagtaccgtt gaatgaagat
     1621 gagagaatct gcggggaagg cgggcctgcc gccagcagca aatcggggcg agggccgcga
     1681 cccgctgcac tcagcccgcc gcgaggattc gctcgaggcg cgtggcgtac gcccggctcg
     1741 cggcgtcccc cgtcaccagc tcgatgtcgc cgggcccgcg cgcgccgagg ttcagctcca
     1801 ccgcccgcac gagctgctcc tgcgcccaga tcccgcggct catgatggcg tcgttggcgc
     1861 ccaagtcctt caagaccgcg agccccacgg cgtccaccgc tacgcgatcg tcacccacga
     1921 tcacgacgtt cccgtccttc cgctcgccgc tcgaggggcc accgtcggtg aaggcggaga
     1981 ccccgtcgag taccacgagt tgcggccggt agccggcatt gagctccgcg atcatgcgcc
     2041 gcatgtccgg cgagcgatgc atcgagcggc ggagcggcct gggggtgagc cccaccgcga
     2101 gcttgagcga catggtgaac acgccgccgt actggtgcgt cttgaggcag caggtggaga
     2161 ccaggtactc ggcctccgta acgagacgcg gcaggtggta cccttcgggc cagtgcgttc
     2221 ccccaacgcc gatgggtatc cagtccgcgt cgggcagttc gtcgaggttg acgacgtcga
     2281 agcgcagggc ggcggcgagg tcgaggacgc cgtactgctc catgacgcgc cgtgtcccgg
     2341 acggcccgct cgactcgccc agcgtcacgg tccgggcgcc ccgctcgtac agctcggtga
     2401 ccagctggcc gagcgtgtcc gggtgcgtcg aggccggcgc gggatcggcg ctgttgaggt
     2461 tcggtttgag caccactcgt ctgcccgcga tcccggcggg ctcgaacaac cggagggcct
     2521 cggtgacccc tttgcggcga tccgaggtac ggaccacgac gactcggctc cgccgcagcg
     2581 tcgaggggat acggagacca cgtgacaccc acgggaggag ggcgatccct gccgaagtcc
     2641 ggaggaaatc gcgacgcgga attatgcggg atcggcgaga cacgctggcc tcccgggcag
     2701 cgaggcgcga tgcgggcgct gcaatgccga acgctgccag cgtatcccat gagatctgaa
     2761 ttgcgaatga accgcggatg aaggccctgg gccggcggag cccgggaatg aaacagcccg
     2821 gtcgcgccgc ggcgttgcat tttgatatct aagtaattac tttccagcca cttacgacgc
     2881 atttccacca catctactat accagctgac ggctcaccgc tgaaagctga cggctcccaa
     2941 tgaccgcacc gaacgcgaga gaacgcatcc tgccgcgcct catcgaggac gagctgaagg
     3001 aatcgttcct cgactattcg atgagcgtca tcgtgcagcg cgcgctgccc gatgtgcgcg
     3061 acggcttgaa gccggtgcac cgccgcatcc tgtacgcgat gaacgagctg gggctcttgt
     3121 cggggcgccc ctacaagaag agcgcgacgg tggtgggcga cgtgctgggc aaataccacc
     3181 cgcacgg
//
LOCUS       DBNGZW010000142         6154 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_217386, whole genome shotgun sequence.
ACCESSION   DBNGZW010000142 DBNGZW010000000
VERSION     DBNGZW010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6154)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6154)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6154
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_217386"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /locus_tag="AC16JC_03490"
     CDS             complement(<1..1588)
                     /locus_tag="AC16JC_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5163791.1"
                     /translation="MNLSGLFIRRPVATTLVMLAILLFGVMGYRLLPVSDLPNVDFPT
                     ITVSAGLPGANPETMAAAVATPLEKQFATIAGIDAMTSSSSQGSTNITLQFNLSRNID
                     AAAQDVQAAISRVLRQLPRDMLPPSFRKANPADSPILFLAVSSTTLSLSALDEYAETF
                     LAQRMSTVDGVAQVSVFGSQKYAVRVQIDPQALVARGIGIDEVAAAVDAGNPNVPTGT
                     LWGPHRAYTVLADGQIQSAPEFRQLVVVYKDGAPVRLQDVARVLDDVQDNRTASWFDG
                     SRAIVLAVQRQPGTNTVAVASAVKRTVEALGPQLPASVRIQTLYDRSESINDSVNDVR
                     VTLLITLCLVVLVIFLFLRNLSATVIPSLALPLSIIGTFAAMFLLGYSLDNLSLMALT
                     LSVGFVVDDAIVMLENIVRHMEMGKRPLQAALDGSREIGFTILSMTLSLTAVFIPVLF
                     MGGIIGRLFHEFAVTIGIAILVSGFVSLTLTPMLSSRFLRPSAQAHHGRIYAVTENGF
                     KKMLQVYEKGLSWSIRHRRVT"
     gene            complement(1585..2571)
                     /locus_tag="AC16JC_03495"
     CDS             complement(1585..2571)
                     /locus_tag="AC16JC_03495"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5163792.1"
                     /translation="MEATGTVEPMRTVAVTSRVGGMLQRVRFAEGEEVGEGQVLFEID
                     PRPFAAALLQAQANMSRDVAQAENAVREAARYQALVKDKYVTEEDYQARQATADAMTA
                     TVRADSAALTVARLNLSYATIRAPITGRTGGLLVHEGNQVVANQATPLVTINQLRPIL
                     VRFAVPATDLPEVQRHSSKTLRVEARPAQDSTAFAGELSFVDNHVDSSTGTVLLKGRF
                     PNRQSRLWPGMFVAVTLVLDVQADALVIPEQALLNAQEGTFVFVVDPDGTAKQRPVTV
                     RRRADSLAVIDAGLEAGMLVVTDGQLRLTPDARVEIRSGPRTEADSPGEGRQ"
     gene            complement(2840..3697)
                     /locus_tag="AC16JC_03500"
     CDS             complement(2840..3697)
                     /locus_tag="AC16JC_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163793.1"
                     /translation="MRTGLLDLVRIGLAALLLHAPGRLEGQRNRMGMMRQADLIFVGT
                     VAAVGAASMVGVPVSQRTLSVTVDAVLEKSAAVRLGAGDTVTVEAAAPGVLAVGQQAT
                     FYTRAWLFGRGIAAQEVGHETMAARRSAAALVLLQDSVSRVRRQVSDSTLQARIRAAD
                     MIVVGRVETIRPASLAAIPGGRRRVTEHDPAWQEAIVVIETTIKGAAPADQRVVVRFP
                     GSLDVAWRTMPRYAVGQEGTFLLRRDRVSGTPTAMIAGRQVTAYAALSTQDVLTKQDA
                     ARVQALARP"
     gene            complement(3694..5145)
                     /locus_tag="AC16JC_03505"
     CDS             complement(3694..5145)
                     /locus_tag="AC16JC_03505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163794.1"
                     /translation="MRIAAVLLAVVVAAGCNGGGGGGSGGGGGGEVQPGTRVVNMIPA
                     SLSGEAAQDAEPFLAVLSGNFAIMAASAFTPNPAGPSSTTAPIFVTQDSGRTWALRAT
                     VPSDVTTADITEAFDGGGGDLYAGILSLSGDLALDELKTGDVFSLTPMSIQASRGNVD
                     QPFVRATTVGTADRVYIGMNDFDAPAGRTATVDVSQNGGSSYTTVRLESRGTADQDGP
                     SIRPGVSADRTVYAAYFGWRSFDGNVATSDVVVVRDDSGAAGSDKFRSLVDPGDGLPG
                     RIVAHNVLIPWSNAPTLGQERIGSTLALAVAPNHSEVVYVAWADRVGSGDIYTIHLRR
                     SIDRGATWSGDIRTVVNATNASLAVAENGTAGFLYQQLSGSGAASRWVTHLERSKDGF
                     VTTSDLVLATVPANTPSVDFLPYLGDYTGLLAVGGELRGVFSANNTPDSTSFPQGVVY
                     QRSVDFASHRLLSGGATVAVSIDPFYFSVPVLP"
     gene            complement(5202..>6154)
                     /locus_tag="AC16JC_03510"
     CDS             complement(5202..>6154)
                     /locus_tag="AC16JC_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163795.1"
                     /translation="YHEPIFDYGNVDDPLAPRSWDTYAPLGWYGTNYAGLRGRIGILS
                     EAYSHADFKTRIQVTHDFVVETLEFAAQHADEIRRLEREADRVTTLEGAGQRPRPALA
                     TAYRLAGRGEEPVVLEVMRPNPDSARGAETPRLLPTGELRTYRLPVNDRFTDSLTQPL
                     PAGYFLPPSAAGIVGLLQLHGVQVARLAESWTDSIEVLGITQLTWSPREFQGHHLLQV
                     TGQYTRESRTLPAGSFYVTTAQPLGRLVFALLEPGCYGLARWGIFDRWLGSEFGAYSG
                     LVYGSGRASEFPVWRALRSPRAAMRVPAQGDDRSPGDLTP"
BASE COUNT          982 a         2181 c         2105 g          886 t
ORIGIN      
        1 tggtcacgcg cctgtgacgg atcgaccagg agaggccctt ctcgtagacc tggagcatct
       61 tcttgaatcc gttttcggtc acggcgtaga tccggccatg atgcgcctgg gctgaggggc
      121 gcaggaaccg gctcgagagc atcggcgtga gcgtcagcga gacgaacccc gacacgagga
      181 tcgcgatccc gatggtcacc gcgaactcgt ggaacaggcg cccgatgatg ccgcccatga
      241 agagcaccgg gatgaacacc gccgtgagcg acagggtcat cgagaggatc gtgaagccga
      301 tctcccgcga cccgtcgagc gccgcctgca gcgggcgctt gcccatctcc atgtgccgga
      361 cgatgttctc gagcatcacg atggcatcgt ccaccacgaa ccccaccgac agcgtcagcg
      421 ccatcagcga caggttgtcg aggctgtagc ccagcaggaa catcgccgcg aacgtgccga
      481 tgatcgacag cggcagggcc aggctcggga tgaccgtcgc cgaaaggttg cgcaggaaca
      541 ggaagatgac gaggacgacg aggcagaggg tgatgagcag cgttacccgg acgtcgttca
      601 ccgagtcgtt gatcgactcc gagcgatcgt acagcgtctg gatacgcacc gacgccggga
      661 gctgggggcc gagcgcctcc accgtccgct tcaccgccga cgctaccgcc acggtgttgg
      721 tgcccggctg ccgctggacg gccagcacaa tggcgcgcga tccgtcgaac cagctcgcgg
      781 tccggttgtc ctgcacgtcg tccagcaccc gcgccacgtc ctgcagccgc accggcgcac
      841 cgtccttgta gacgaccacg agctgccgga actcgggcgc gctctggatc tggccgtcgg
      901 ccagcacggt gtaggcgcgg tgcggacccc acaaggtgcc ggtggggacg ttcggattgc
      961 ccgcgtccac cgcggccgcg acctcgtcga tgccgatacc gcgcgccacc agcgcctgcg
     1021 gatcgatctg cactcgcacg gcgtacttct gcgagccgaa caccgatacc tgggccacgc
     1081 cgtccacggt cgacatgcgc tgggcgagga acgtctcggc gtattcgtcc agcgccgaca
     1141 gggacaaggt cgtcgagctc accgcgagga acagaatcgg cgagtccgcg ggattcgcct
     1201 tgcggaacga gggggggagc atgtcccgcg gcagctggcg caggacccgc gagatggccg
     1261 cctgcacgtc ctgcgccgcc gcgtcgatgt tgcgggacag gttgaactgc agcgtgatgt
     1321 tcgtgctccc ctgcgagctc gacgacgtca tcgcgtcgat gccggcgatc gtcgcaaact
     1381 gcttctcgag cggcgtcgcg accgcggcgg ccatcgtttc cgggttggcc cccggcaggc
     1441 cggccgacac ggtgatcgtg gggaaatcca cgttcggcag atcgctcacc ggcagcaacc
     1501 ggtagcccat cacgccgaac agcagaatcg ccagcatcac gagcgtggtc gcgaccggcc
     1561 ggcgtatgaa cagccccgag aggttcactg gcgaccctcc cccggcgaat ccgcctcggt
     1621 ccgagggccg ctgcggatct cgacccgggc gtccggggtg agccgcagct gcccgtccgt
     1681 cacgacgagc atccccgcct cgagtccggc gtcgatcacc gccaggctgt ccgcccggcg
     1741 ccgcacggtg accggccgct gcttggcggt cccgtcgggg tcgaccacga acacaaacgt
     1801 tccctcctgc gcgttgagca gcgcctgctc cggaatcacc agcgcgtcgg cctggacatc
     1861 cagcaccagc gtcacggcga cgaacatgcc gggccacagc cggctttggc gattggggaa
     1921 ccggcccttc aacagcacgg tgccggtgct cgagtcgacg tgattgtcca cgaacgacag
     1981 ctcgcccgcg aacgccgtcg aatcctgcgc cggccgcgct tccacccgca gggtcttgct
     2041 gctgtgccgc tgcacctcgg ggaggtcggt cgccggcacg gcgaaccgca ccaggatcgg
     2101 ccgcagctgg ttgatggtga cgagcggcgt cgcctggttg gcgaccacct ggttgccctc
     2161 gtgtaccagc aggccgccgg tgcggccggt gatcggggcc cggatggtgg cgtaggagag
     2221 attcagccgg gccaccgtca gcgccgccga atccgcccgc accgtcgccg tcatggcgtc
     2281 ggccgtcgcc tgccgcgcct ggtaatcctc ctccgtgacg tacttgtcct tcacgagcgc
     2341 ctgatagcgt gcggcctccc gcaccgcgtt ctccgcctgc gcaacgtccc ggctcatgtt
     2401 ggcctgggcc tgcagcagcg ccgcggcgaa cggccgcgga tcgatttcga acaggacctg
     2461 cccctccccg acctcctcac cctcggcgaa gcgcacgcgc tgcagcatgc cgcccacacg
     2521 cgaggtcacc gcgacggtac gcatcggctc gaccgtgccg gtcgcctcga tctcttaggg
     2581 gacggcccgg cgctcgaccc gggccacgct caccggtatc ctgcctgggg gcccattcgg
     2641 gcgcgacgaa caggccgggg ccagcacggc cgggagggcg cagagcagag cagactggag
     2701 tcgggacacg ggcgtatgca cttccctgac acagggtgag tcgcgacggc gaccgtatgc
     2761 ttacgcacaa cccggccccc gcgtggaggc acgcgcgggc gcgggcagcg gataaccgct
     2821 ggaagatacc gggtttggct tacggccgcg ccagggcctg cacccgcgca gcgtcctgtt
     2881 tggtcaggac atcctgggtg ctgagcgcgg cataggcagt gacctgtcgg ccggcgatca
     2941 tcgccgtggg cgtgcccgag acccgatccc gccgcagcag gaacgtcccc tcctgcccca
     3001 cggcgtaccg gggcatggtg cgccaggcga cgtcaagcga ccccgggaaa cgcacgacga
     3061 cccgctggtc ggccggggcg gcgcccttga tcgtggtctc gatgaccacg atcgcctcct
     3121 gccaggcggg atcgtgctcc gtgacccggc gccgcccccc cgggatcgcg gcgagcgacg
     3181 cgggccggat ggtctcgacg cgtccgacga cgatcatgtc ggcggcgcgg atccgcgcct
     3241 gcaacgtcga gtcgctcacc tgacgccgca cccgcgacac ggaatcctgc agcagcacga
     3301 gcgccgccgc cgagcggcgc gccgccatcg tttcatggcc gacctcctgg gccgcgatac
     3361 cgcgcccgaa cagccacgcc cgagtataga acgtggcctg ctgccccacc gcgagcaccc
     3421 cgggagccgc cgcttcgacc gtcacggtgt cgcccgcccc cagtcgcacc gcggccgact
     3481 tctcaagaac cgcgtcgact gtgaccgaga gagtacgctg ggagacgggg acgccgacca
     3541 tcgaggcggc tccgacggcg gcgacggtcc cgacgaagat caggtcggcc tgacgcatca
     3601 tgcccatccg gttgcgctgc ccctccagcc gcccaggcgc gtggagcagc agcgccgcca
     3661 ggccgattcg caccagatcg agcaagcccg ttctcatggc agtaccggta cgctgaagta
     3721 gaacggatcg atggagacgg cgaccgtagc gcctccgctc agcagacggt ggctggcgaa
     3781 gtccacgctc cgttgataca ccacgccctg ggggaaggag gtggagtccg gcgtgttgtt
     3841 cgcggagaac accccgcgca gctccccgcc caccgccagc agcccggtgt agtcgcccaa
     3901 atagggaagg aaatcgacgc tcggcgtatt cgccggcacg gtggcgagca ccaggtcgct
     3961 ggtcgtgacg aagccgtcct ttgatcgctc gaggtgagtg acccaccgac tcgccgcgcc
     4021 gctgccgctg agctgctgat acaggaaccc cgcggtgccg ttctcggcca ccgcgaggct
     4081 ggcgttggtg gcattcacca cggtccgaat gtcgccgctc caggtagcgc cgcggtcgat
     4141 cgagcggcgc agatggatgg tgtagatgtc ccccgagccg acgcggtccg cccaggcgac
     4201 gtagacgacc tcgctgtgat tgggcgcgac ggcgagggcg agcgtggagc cgatccgctc
     4261 ctggccgagg gtgggggcat tggaccaggg gatcagcacg ttgtgcgcga cgatccggcc
     4321 cgggagcccg tcacccgggt ccaccaggct gcggaacttg tcgctgccgg cggcgccgga
     4381 atcgtcccgg accacgacca cgtcgctcgt cgccacgttc ccgtcgaagc tccgccaccc
     4441 gaagtacgcc gcgtacacgg tgcgatcggc ggagaccccg gggcggatcg acggaccgtc
     4501 ctggtcggcg gtgccgcggg attcgaggcg gacggtggtg tagctcgacc cgccgttctg
     4561 cgacacgtcc acggtggccg tgcgtcccgc cggcgcgtcg aaatcgttca tgccgatgta
     4621 gacgcggtcg gcggtgccga cggtggtggc acgtacgaac ggctggtcca cgttgccccg
     4681 ggatgcctga atactcatcg gcgtgagcga gaagacgtca cccgtcttca gctcgtcgag
     4741 cgccagatcg cccgacagcg acaggatgcc ggcgtagagg tcgccgccgc caccgtcgaa
     4801 ggcttcggtg atatcggccg tcgtcacgtc gctggggacg gtcgcccgca gcgcccaggt
     4861 ccggccgctg tcctgcgtga cgaagatggg agcggtggtg ctggatgggc cggcggggtt
     4921 gggcgtgaat gccgaggccg ccatgatcgc gaagttcccg gagagcaccg ccaggaacgg
     4981 ctcggcatcc tgagcggcct cgccactcaa cgacgccggg atcatgttca ccacccgggt
     5041 gccgggctgc acctctcctc ccccgccacc cccgctccct cctccacctc ccccgttgca
     5101 gcctgcggcg acgacgacgg cgaggagaac cgctgcgatc cgcatgatcc ggcctcatgg
     5161 gtgcgagcca gcgagcaagt tgcgccgccg tgtggcggtg ctcaaggggt caagtcacca
     5221 ggcgaccggt catcgccctg cgccggcacc cgcatcgcgg cgcgcggaga ccgcagcgcg
     5281 cgccacaccg ggaattcgct cgcccgaccg gagccgtaga cgagaccgga ataggctccg
     5341 aactccgacc ccagccagcg gtcgaaaatc ccccagcgcg ccaggccgta gcatccgggc
     5401 tcgagcaacg cgaacaccag ccgcccgagc ggctgagcgg tggtcacgta gaagctgccg
     5461 gccggcagcg tgcggctctc cctggtgtac tgtcccgtca cctggagcag gtggtgcccc
     5521 tggaactccc gggggctcca ggtgagctgg gtgatcccga ggacctcgat gctgtcggtc
     5581 caggactccg cgaggcgcgc cacctgtacg ccgtgcagct gcagcaaccc gacgatcccg
     5641 gcggccgacg gcggcaggaa atagcccgcc gggagcggct gggtgagcga atcggtgaag
     5701 cggtcgttga cgggaagccg gtaggtccgc agctcgcccg tcggcaggag gcgcggggtt
     5761 tcggctccac gcgccgagtc gggattgggg cgcatcactt cgagcaccac cggctcctct
     5821 ccacgacccg cgagtcgata agccgtggcg agcgccggcc gcggccgctg ccccgcgccc
     5881 tcgagcgtcg tcacgcgatc ggcctcccgc tccagacggc ggatctcgtc ggcgtgctgc
     5941 gccgcaaact ccagcgtttc cacgacgaag tcgtgcgtca cctggatccg cgtcttgaaa
     6001 tcggcgtggg aatacgcctc ggacaggatg ccgatccgcc cccgcagccc tgcgtagttg
     6061 gtcccgtacc agccgagtgg ggcgtaggtg tcccagctcc tgggcgcgag ggggtcgtcc
     6121 acgttgccgt aatcgaagat cggctcgtga tagc
//
LOCUS       DBNGZW010000143         2496 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1444923, whole genome shotgun sequence.
ACCESSION   DBNGZW010000143 DBNGZW010000000
VERSION     DBNGZW010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1444923"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..770)
                     /locus_tag="AC16JC_03515"
     CDS             complement(<1..770)
                     /locus_tag="AC16JC_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5163796.1"
                     /translation="MVDARSSYGEQIETARTALSRGDRPAAAAAFRSAIDVARSDAGL
                     QRELLTALVQLGKLEQELGRPGEAERLLTEALETGERKFGTQHVSLAPVLNELSRLHI
                     RQSDHALAEVVLQRLLRITRTRGEDHPDVATALAGLGLVMRALGDDDSAEQRYREALR
                     IREKSLPPQHMATVVTMEQLSETCAARGNLAEALALLQRALPTREAALGSEHATVRAL
                     RSRIAALELRAARAAAPSPERQPVAPSVARTPAPAPLPT"
     gene            1163..1447
                     /locus_tag="AC16JC_03520"
     CDS             1163..1447
                     /locus_tag="AC16JC_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="immunoglobulin domain-containing protein"
                     /protein_id="HIR5163797.1"
                     /translation="MDFLQFQDDGRTLTCRSASSPATPGTTWWWLEVTGETQRYAAFR
                     TEPTDTPENLRPRMIAHYAHLLAERARPATFRPRWSRPAAAAPAPANPAP"
     gene            1635..2126
                     /locus_tag="AC16JC_03525"
     CDS             1635..2126
                     /locus_tag="AC16JC_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163798.1"
                     /translation="MRRLVEACAVIVCLAGCSGASDTITDVDAPFGGVYRLRQVNEAS
                     LPLYFWSLWYPGRASGPNVQSSTLVAAELSVLSDGSFVWSTLLDEVATPSESLLPQYV
                     VWQVRRNAYGTWSYTPATGTVSLTGFDQSGAYVLVGSTASGVVTLSSTSTNTPNLTFV
                     LER"
     gene            complement(2152..>2496)
                     /gene="ligD"
                     /locus_tag="AC16JC_03530"
     CDS             complement(2152..>2496)
                     /gene="ligD"
                     /locus_tag="AC16JC_03530"
                     /inference="COORDINATES: protein motif:HMM:NF045432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase LigD"
                     /protein_id="HIR5163799.1"
                     /translation="GELLARVMLREVEDIATVTRQVTKRGDKVYLDYLQNRHGQTIVA
                     PFSVRPLPGATVSMPLRWDEVVDGLNPKDYTIRNAMDRMEALGQDPVAPVLSEIPDLG
                     EALGRLAALLTT"
BASE COUNT          367 a          878 c          841 g          410 t
ORIGIN      
        1 gtcggtagtg gcgccggggc aggggtccgt gccacggagg gtgcgaccgg ttgccgttcg
       61 ggcgaggggg cagcggccct cgcggcccgg agctcgagcg cggcgatgcg cgaccgcagc
      121 gcacgcacgg ttgcatgctc gctccccagc gccgcctcgc gcgtcgggag ggcgcgctgg
      181 agcagcgcga gcgcttcggc gagattgcca cgcgccgcac acgtctccga gagctgctcc
      241 atcgtgacca cggtcgccat gtgctgcggc gggagcgatt tctcgcgaat gcgaagcgct
      301 tccctgtagc gctgttcggc cgagtcgtcg tctcccagtg cgcgcatcac gaggccgagg
      361 cccgccagcg cggtggcgac gtcgggatga tcctcgcccc gggtccgggt gatccgcagc
      421 agccgctgga ggacgacctc ggcgagcgcg tgatccgact gccggatgtg cagccggctc
      481 aactcgttga gcaccggggc gagcgacacg tgctgggtgc cgaacttccg ctccccggtc
      541 tcgagcgcct cggtgagcag tcgctcggct tccccagggc gacccaactc ctgctcgagc
      601 ttcccgagct gcaccagtgc ggtgaggagc tcccgctgga gacccgcatc gcttcgcgcg
      661 acgtcgatcg ccgaacggaa tgcggcggcg gccgcgggtc ggtcgccgcg gctcagtgcc
      721 gttcgcgctg tctcgatctg ctccccgtac gacgatctcg cgtcaaccat ctgccacgtc
      781 ctcacgtagt gctgcttccg cggccctccg tcccatcggc gatcgacccg gcagaggtgt
      841 gaacccaccg ccgaccgcat tgggagacga gcggtgatgg gccagcgata cgcggacccg
      901 gggcggtggg gctcccctgc ggtcgagccc gtgttccgtc cacggtccgg gaggtgcctc
      961 gcgtccttgt tgggaggtgg accaccggct aacttcattg agagacgaga gtcgcatcgc
     1021 agcacgacat cagcctggag tcggcacgaa agtccgactc gagcgaacga tccaacgggc
     1081 ccggcactgt tccgggcctt tttcgttggt cgacgcgacc cgcgcctcga aatccccctc
     1141 gaattccccc tcaggagtgc cgatggactt ccttcaattc caggacgacg ggcgcacgct
     1201 cacctgccgc agcgcgtcgt cgccggccac gcccggcacc acgtggtggt ggctcgaggt
     1261 cacgggggag acgcagcggt acgccgcctt ccggaccgag ccgaccgaca cgcccgagaa
     1321 cctgcgcccg cgcatgatcg cgcattacgc gcatctgctg gccgaacgtg cgcgccctgc
     1381 gacgttccgt ccgcgctggt ctcgtcccgc cgcggcggcc ccggcgcccg cgaaccccgc
     1441 gccctgagcg gcggctcatc gcgcggcgga gggccctcgc cgcgcgtgag ccctgccggt
     1501 cggtctacgg catgaggtag ccgacctcgg tcacggcccc tggcgaaagg ccactcatcg
     1561 gagagaagat tgggctgccg aacgcccacc cgaggcgcac gcgcagtcgc agcgattccg
     1621 aacgagggcc agcgatgcga agactggtcg aagcatgcgc cgtcattgtc tgcctcgctg
     1681 gctgcagcgg ggcgagtgac acgatcaccg atgtcgatgc cccgttcggc ggcgtctaca
     1741 gacttcgcca ggtgaacgaa gcttcactcc cgctctactt ctggtccttg tggtacccgg
     1801 ggcgggcaag cggcccgaac gtgcagagca gcacgctggt cgcggccgaa ctttccgttc
     1861 tttcggacgg ctcgttcgtg tggtccacgc tgctcgacga agttgcgaca ccgtccgagt
     1921 cgctgctgcc tcaatacgtt gtctggcagg ttcgccgaaa tgcgtacgga acgtggtcgt
     1981 atacgcctgc gacggggact gtctccctga cggggttcga ccaatccggc gcttatgtcc
     2041 ttgtcggatc gacggcgagt ggcgtcgtga cgctttcctc cacgtccacg aacacgccga
     2101 atctgacgtt cgtgctcgag cggtagggcg ggagagaccg ccacggccgc atcaggtggt
     2161 cagtagcgcc gcgagccttc cgagcgcctc accaagatcg ggaatctcgc tcaacaccgg
     2221 ggccaccgga tcctggccga gcgcctccat tcggtccatc gcattgcgga tcgtgtagtc
     2281 cttcgggttc agcccgtcga ccacctcgtc ccaccgcagc ggcatcgaca ccgtggcgcc
     2341 gggaagggga cggacgctga acggcgcgac gatcgtctgg ccgtgccggt tctgcagata
     2401 gtcgagatac accttgtcgc cgcgcttcgt gacctggcgc gtgaccgtcg cgatgtcctc
     2461 cacctcgcgc agcatcacgc gcgcgagcag ctcgcc
//
LOCUS       DBNGZW010000144         3121 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1513165, whole genome shotgun sequence.
ACCESSION   DBNGZW010000144 DBNGZW010000000
VERSION     DBNGZW010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1513165"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="AC16JC_03535"
     CDS             complement(<1..445)
                     /locus_tag="AC16JC_03535"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5163800.1"
                     /translation="MTTACTADHCACVTGGELGVALSGGGARGLAQIGVLKVLERARV
                     PVSFVAGTSAGAVIGAVYATSETALQAEQRILEHLATTGIGFNTETFETLAKSNARRP
                     AGLLGLLGTLRRAARPGGSLIGGDAMRASLRRLLGEATFARARIPF"
     gene            complement(479..>3121)
                     /locus_tag="AC16JC_03540"
     CDS             complement(479..>3121)
                     /locus_tag="AC16JC_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011288003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5163801.1"
                     /translation="QDWYVRYQLTAVPGVSEVASLGGFVRQYQVEVDPERLRAFNIPV
                     MRVVQAIGTHNADVGARVIEMGGREYMIRGLGYLRTVDDIGNVAVGSTMSGTPIRVSD
                     VATVHIGPAPRRGAADLNGRGEVVAGIVIMRFGSDALKTIEAVKTRIAEIQRTLPAGV
                     RLVPVYDRSDLIHRAIGNLQRTLLEESLIVAAIAVLFLLHLRSALVAVVTLPLGILVS
                     FLVMRAIAVTANIMSLGGIAIAIGAMVDAAIVMIENMHKHLERAEGRPRWDVVLEATK
                     QVGPPLFFSLLIITVSFLPVFSLEQQEGRLFRPLAFTKTFAMAGAALLSVSLVPVLMG
                     YLVRGRIHPQEANPVNRWLMRVYRPVIGWVLRHPWRVLAGAGVVLAATVLPWSRLGSE
                     FMPPLDEGSLLFMPTTVPGVSIAQAQEIMRHQDSVLASFPEVAAVLGKVGRANTATDP
                     APLDMYETTIILQPRKRWRPGMTTERLVSAMDQAVRLPGVTNAWTMPIKNRIDMLATG
                     VRTPVGVKVYGPNLDTLQRLGERVERILQKVPGTRNAFAERNVSGYYVDVDIDRAQAA
                     RYGLNAGDLHDAIMATAGGMTAAITVEGRERYEVNVRYPRELRDDLDKLRAIVIPTMT
                     GIQIPLGQVAAVNVVQGPMAVKTDNAFPVTTVSVDIAGRDLGGYVREAQRVVAQELTL
                     PAGYRLAWSGQFEAMQRVREKLKLLVPLTLGLIFLLLYLNFHSVPHALIVMLALPFAL
                     VGGVWSIWLLGYNTSVAVWVGFIALAGVAAETGVIMLIYLDEAYHHRRARGQMNDPAG
                     VAAAVHEGAVERLRPKMMTVVAIMAGLAPILWGGGTGADVMKRIAAPMVGGMITSTVL
                     TLAVIPAIYYLWRRWQLGREPRGS"
BASE COUNT          517 a         1093 c         1043 g          468 t
ORIGIN      
        1 cgaaggggat ccgtgcgcgg gcaaacgtgg cttcaccgag caggcgacgc agcgaagccc
       61 gcatcgcgtc tcccccaatg aggctgcccc ccggtcgggc ggcacgccgc agggtgccca
      121 gcaagccgag cagaccggcc gggcggcgag cgttggactt cgcaagcgtt tcgaaggtct
      181 ccgtgttgaa cccgattccg gtggtcgcca ggtgctcgag aatgcgctgc tccgcctgca
      241 gcgccgtctc cgaggtcgcg tagaccgccc cgatgacggc cccggcactc gttcctgcga
      301 cgaaggagac ggggacgcgt gctcgctcga gcactttgag cacaccgatc tgagccagtc
      361 cccgagcgcc cccgcccgag agcgcgactc ccaactcccc gccggtgacg caggcgcaat
      421 ggtcagccgt gcaagccgtg gtcatagggc actcctttcg tcgcgctgcg cgaacccgtc
      481 aagaaccgcg gggctcccgc ccgagctgcc accggcgcca caggtagtag atcgccggaa
      541 tcaccgccag cgtgagcacg gtggacgtga tcattccgcc taccatcggc gcggcgatcc
      601 gcttcatcac gtcggcaccg gtgccgccgc cccacaggat cggcgcgagg ccggccatga
      661 tcgcgaccac ggtcatcatc ttcggccgca gccgctccac cgcgccttcg tgcaccgccg
      721 ccgcgacgcc tgccggatcg ttcatttgcc cgcgcgcgcg ccggtggtga tacgcctcgt
      781 ccaggtagat gagcatgatc accccggtct cggcggcgac gcccgccagc gcgatgaatc
      841 ccacccacac cgccacgctc gtgttgtagc ccagcagcca gatggaccag actcccccga
      901 ccagggcgaa cggcagtgcg agcatcacga tcagtgcatg gggcacgctg tggaagttga
      961 gatacaacaa cagaaagatg agcccgagcg tcaacggcac gaggagtttc agcttctcgc
     1021 gcacccgctg catcgcctcg aattgcccgc tccacgcgag gcgatagccg gccggtaggg
     1081 tcaattcctg cgcgacgacg cgctgcgcct cgcgcacata gccgccgagg tcgcggccgg
     1141 cgatgtccac ggacacggtc gtgacgggaa acgcgttgtc ggtcttcacc gccatcggtc
     1201 cctggacgac gttgacggcg gcgacctgcc ccagcgggat ctgaatcccg gtcatggtgg
     1261 ggatgacgat ggcgcggagc ttgtcgaggt cgtcgcgcag ctcccgtggg tagcggacgt
     1321 tgacctcgta gcgttcgcgc ccttcgacgg tgatggccgc ggtcatgccg ccggcggtcg
     1381 ccatgatggc gtcgtgcagg tcgccggcgt tgagcccgta gcgcgcggcc tgggcccggt
     1441 cgatgtccac gtccacgtag tatcccgaaa cgttgcgctc cgcgaaggcg ttgcgggtcc
     1501 cgggaacctt ctggagaatc cgttcgacgc gctcgccgag ccgttgcagc gtgtccaggt
     1561 tggggccata gaccttcacg ccgacgggtg tgcgcacgcc ggtggcgagc atgtcgatgc
     1621 gattcttgat cggcatcgtc catgcgttgg tcacgcccgg caggcgcacc gcctggtcca
     1681 tcgcgctcac gaggcgttcc gtggtcatgc ccggccgcca ccgcttccgg ggctgcagga
     1741 tgatcgtcgt ctcgtacatg tcgagcggcg ccggatccgt tgcggtgttg gcgcggccca
     1801 ccttgccgag cacggcggcc acctccggaa acgaagcgag gacgctgtcc tggtgccgca
     1861 tgatctcctg cgcctgggcg atggacacgc cggggacggt cgtcggcatg aagagcaacg
     1921 acccttcgtc gagcggaggc atgaactcgc tgccgagccg gctccagggg agcacggtcg
     1981 ccgccagcac cacgccggcg ccggccagga cgcgccaggg atggcgcaac acccacccga
     2041 tcaccgggcg atacacccgc atcagccagc gattgaccgg gttggcctcc tgcgggtgga
     2101 tcctgccgcg cacgaggtac cccatcagca ccggcaccag ggacaccgag agcagggccg
     2161 ccccggccat ggcgaacgtc ttggtgaagg cgagcggcct gaacagccgc ccctcctgtt
     2221 gctcgagcga gaacacgggc aggaacgaga cggtgatgat gagcaacgag aagaacagcg
     2281 gcggtccgac ctgcttcgtg gcctccagca cgacgtccca gcgcggacgg ccctcggcgc
     2341 gctccaggtg cttgtgcatg ttctcgatca tcacgatggc cgcgtccacc atcgctccga
     2401 tggcgatcgc gatcccgccc agactcatga tattggcggt cacggcgatg gcgcgcatca
     2461 cgagaaacga aaccaggatg ccgagcggca acgtgacgac cgccaccagc gccgagcgca
     2521 ggtgcagcag aaagagcacg gcgatggctg cgacgatgag cgactcctcg agcagtgtgc
     2581 gttgcaggtt gccgatcgcg cgatggatca ggtcggagcg gtcgtacacg gggacgaggc
     2641 gaactccggc cggcaacgtg cgctggatct cggcgatgcg cgtcttcacc gcctcgatgg
     2701 tcttgagcgc gtccgagccg aatcgcatga tcacgatgcc cgccacgacc tcgccgcggc
     2761 cgttcaggtc cgcggcgccg cggcgcgggg cgggcccgat gtgcacggtg gccacgtcgg
     2821 acacgcggat cggtgtgccg ctcatggtcg agccgaccgc gacgttcccg atgtcgtcca
     2881 ccgtccgcag gtagccgagc ccccggatca tgtattcgcg accgcccatc tcgatgaccc
     2941 gcgcacccac gtcggcgttg tgggtgccga tcgcctgcac gacgcgcatc accgggatgt
     3001 tgaacgcgcg caaccgctcg gggtccacct cgacctggta ctgccgcacg aatccgccga
     3061 ggctcgccac ctccgagacc cccggaacgg cggtcaactg gtagcggacg taccagtcct
     3121 g
//
LOCUS       DBNGZW010000145         2574 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1513197, whole genome shotgun sequence.
ACCESSION   DBNGZW010000145 DBNGZW010000000
VERSION     DBNGZW010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2574
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1513197"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="AC16JC_03545"
     CDS             complement(<1..815)
                     /locus_tag="AC16JC_03545"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5163802.1"
                     /translation="MVRRFGLLHATALNMTNMIGIGPFITIPLLMSALGGPQAMLGWL
                     VALVIVLCDSTVWSELGAALPGSGGSFGYLRHGYGEERVGRLMGFLFVWQFVLSGPLE
                     IASGYIGFSQYLGYIWKGITARQTMLVVTIVGLVNLALLYRRIKSIAMITVALWVGTI
                     VTVVAVIATGARHFDPKVAFDFPPGAFNFSLGFLMGLGAASRIGVYDYLGYYDVCYIG
                     DEVENPGRVIPRSILISTIAVALIYLAINLSIIGVVPWRDFVPAAVHPHSSFVV"
     gene            complement(827..2371)
                     /locus_tag="AC16JC_03550"
     CDS             complement(827..2371)
                     /locus_tag="AC16JC_03550"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012372859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR5163803.1"
                     /translation="MKHRVLVHVLRGLLGFVAVSAAAGQGALPRSTPERQGISSTAVL
                     AFVERADSEIDAMHSFMLLRHGRVVAEGWWSPYDAATPHMLYSLSKSFTATAVGLAIA
                     EGKLSLDDQVLKFFPEDAPAQPSGNLQAMRVRDLLRMNTGQQTEAALWPWSDQPDTAT
                     RDEAWTKTFLAHPVPFKPGTHFQYNSPATYMLSSIVQKVTGMTVLEYLGPRLFEPLGI
                     AHPVWSASPQGVTLGALGLLIRTEDIARFGQLYLQKGMWGGRQLIPASWVAAATALET
                     SNGSSPESDWDQGYGYQFWRSRHNSFRGDGAFGQYCLVLPDVDAVVVITSGVRDMQAV
                     MNLVWETLLPAMRRNALPENAAAVRRLTAKLAGLTLRTPAGSPTGPLAARTSRRWYQF
                     PENARGIQAVALDFAPDSLALVVRTAGGETRTPIGTGSWVKSRGGFTGGLDRLLSVPP
                     HPLIAASGAWTADDVFTVKLVLYETPYSATVTFRFDGDRVLLDEEYNVSFGPTKQAQL
                     IGQGAR"
     gene            complement(2385..>2574)
                     /locus_tag="AC16JC_03555"
     CDS             complement(2385..>2574)
                     /locus_tag="AC16JC_03555"
                     /inference="COORDINATES: protein motif:HMM:NF015153.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5163804.1"
                     /translation="GGVHCSVELPGFTLRYTTDGSEPTARSAVVRGPIPLHGTIRVAA
                     FNTTGRKGHTALLAGHAP"
BASE COUNT          446 a          864 c          878 g          386 t
ORIGIN      
        1 acgacgaagc tcgaatgcgg atgtaccgcc gccgggacga agtcccgcca cgggacgacg
       61 ccgatgatcg agagattgat cgcgaggtag atcagcgcga ccgcgatggt gctgatgaga
      121 atcgatcggg ggatcacccg gccgggattc tcgacttcgt ccccgatgta acagacatcg
      181 tagtagccga ggtaatcgta gacgccgatc cgcgacgcgg cgccgagacc catcagaaag
      241 cccagggaga agttgaacgc gcccggcgga aagtcgaagg cgaccttggg gtcgaagtgc
      301 cgggcgccgg tcgcgatgac cgcgacgacg gtgacgatcg ttcccaccca gagcgcgacg
      361 gtgatcatgg cgatcgactt gatccgccgg tagaggagcg cgaggttcac cagtccgacg
      421 atcgtcacga cgagcatcgt ctggcgcgcg gtgatcccct tccagatgta gcccaagtac
      481 tgcgagaagc cgatgtaccc ggaggcgatc tcgagcgggc cactgagcac gaactgccac
      541 acgaagagaa aacccatcag gcggccgacc cgctcctccc cgtagccgtg gcgcaggtac
      601 ccgaacgagc cgccggatcc gggcagcgcc gcccccagct cgctccacac ggtgctgtcg
      661 cacaggacga tcaccagggc cacgagccag ccgagcatcg cctgcgggcc gccgagcgcg
      721 gacatgagca gcgggatcgt gatgaacggt ccgatcccga tcatgttcgt catgttgagg
      781 gccgtggcgt gcagcaggcc gaaccggcgg accatcgagg gcgcgttcag cgggcgccct
      841 ggccgatcag ctgcgcctgt ttcgtggggc cgaaggacac gttgtactcc tcgtccagca
      901 gcacccggtc accgtcgaag cggaacgtca ccgtcgcgct gtacggcgtt tcgtagagca
      961 ccagcttgac ggtgaagacg tcatccgcgg tccacgcgcc gctggcggcg atcaggggat
     1021 gtggcggtac gctcagcaac cggtcgagac cgccggtgaa gccgccgcgg ctcttcaccc
     1081 aggagccggt cccgatcggg gtccgcgtct cgccgccggc ggtgcgcacc acgagcgcga
     1141 gggaatccgg ggcgaagtcc agcgccaccg cctggatgcc gcgggcattc tcggggaatt
     1201 ggtaccagcg ccgcgacgtc cgggcggcga gcggaccggt cgggctcccg gcgggagtcc
     1261 ggagcgtcaa tccggcgagc ttcgcggtga gccggcgcac ggcggcggca ttctccggca
     1321 gagcgttcct ccgcatcgcc ggcagcagcg tttcccacac aaggttcatc accgcttgca
     1381 tgtcgcgcac gccgctcgtg atcacgacca ccgcgtccac gtcggggagc accaggcagt
     1441 attgcccgaa cgcgccgtcg ccgcgaaacg agttgtgacg ggagcgccag aactgatagc
     1501 cgtaaccctg gtcccagtcg ctctccgggg aagagccatt cgaggtctcc agggcggtcg
     1561 ccgccgcgac ccagctagca gggatgagct gccgcccccc ccacattcct ttctggagat
     1621 acagctgtcc gaagcgggcg atgtcttccg tgcggatcag caggcccagg gctcccagcg
     1681 tgactccctg cgggctggcc gaccacaccg ggtgcgcaat ccccaggggc tcgaacagcc
     1741 gcggcccgag gtactccaac accgtcatac cggtcacttt ctggacgatg ctcgagagca
     1801 tgtaggtggc gggcgagttg tactggaagt gcgtgcccgg cttgaacggg acggggtggg
     1861 caagaaacgt cttcgtccac gcctcatccc gcgtcgctgt gtcgggctgg tccgaccacg
     1921 gccagagtgc cgcctccgtt tgctgacccg tgttcatgcg caacaggtcg cgcacccgca
     1981 tcgcctggag attgccggac ggctgggccg gtgcatcctc cgggaagaac ttcaggacct
     2041 ggtcgtcgag gctgagcttc ccttccgcaa tcgccagacc gacggctgtg gccgtgaagc
     2101 tcttgctgag cgagtacagc atgtgcggcg tcgccgcgtc gtacgggctc caccatcctt
     2161 ccgccacgac gcgcccgtgc cgcaacagca tgaagctgtg catcgcgtcg atctcgctgt
     2221 cggcccgctc gacgaaggcg aggacggcgg tcgatgaaat gccctgacgc tcgggagtgc
     2281 tgcgcggcaa ggctccctgg cctgccgccg ccgagacggc gacgaaccca agcagccccc
     2341 gcagcacgtg aaccagcacg cggtgtttca tggtcacgcc ccgatcacgg agcgtgtccg
     2401 gcgagcagcg cggtgtgacc ctttctgcct gtggtgttga acgcggcgac gcggatggtc
     2461 ccgtggagcg ggatcggacc gcggacgacg gcgctgcgcg cggtgggctc gctgccgtcg
     2521 gtcgtgtagc gcagcgtgaa gccgggcagc tccacgctgc agtggacccc cccg
//
LOCUS       DBNGZW010000146         4935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1713245, whole genome shotgun sequence.
ACCESSION   DBNGZW010000146 DBNGZW010000000
VERSION     DBNGZW010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1713245"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="AC16JC_03560"
     CDS             complement(<1..514)
                     /locus_tag="AC16JC_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020767972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR5163805.1"
                     /translation="MIDAITKTLGDKADYFLKFDKPKIARERLHLPGPDWVDRIFAPS
                     DRSPRVLVNLQRMYGTGRLAGTGYLSILPVDQGIEHSAGASFAKNPDYFDPENIVRLA
                     IEGGCNAVASTFGVLGVAARKYAHKIPFIVKINHNELLTYPNKFDQILFGTVQQAVDM
                     GAAAVGATIYF"
     gene            complement(570..1211)
                     /locus_tag="AC16JC_03565"
     CDS             complement(570..1211)
                     /locus_tag="AC16JC_03565"
                     /inference="COORDINATES: protein motif:HMM:NF015423.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HIR5163806.1"
                     /translation="MPFSVPPLIDLGAVLVGGLSGTLLAVRKQFDLVGCVSLAIVAGV
                     GGGVIRDVLLQQGPPAALQDSRYLLTAVVAAAVGFYRSGSVVRVGRILIAIDALYLAV
                     YTVVGALKALGAGLAPVPAVFLGIVTAVGGGILRDVLAGEAPTLFRTGELYATVSATG
                     SALFVALWESAMVSHAVAGIVAMIVMVVLRLLAVHYGWKAPLPPAGVPGAGLA"
     gene            1216..2328
                     /locus_tag="AC16JC_03570"
     CDS             1216..2328
                     /locus_tag="AC16JC_03570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR5163807.1"
                     /translation="MNVRRWRGGTEAGLFRGLMDRARTFLDGLIARSQVPGLQYLALD
                     RDRTLFEYAGGWADIRHRVPMTADTTMMAYSMSKTITAVAVLQLVAAHKVRLDDPIER
                     YHPTPYGPGITVRHLLAHLSGIPNPIPLRWVHAAAQHAGFDEHAALEAVLRAHPRAAA
                     APGAKYRYSNIGYWLLGSVVERASGQSFASYVAERGFAPLEIAPAALGYTIPDAGHHA
                     RGYLEKYSFMNLANRLLIGRQFIGDYEGAWLRIEPHYPNGPAFGGLVGTARGFGAFLR
                     DQLRPRSVLFDNPARELLYTPQRTRAGRVVPMGLGWHLGDLDGIPFFYKEGGGGGFHC
                     EMRVYRVNRIATVVMTSATGFDVKRCLTSLDRQFLG"
     gene            complement(2297..3961)
                     /locus_tag="AC16JC_03575"
     CDS             complement(2297..3961)
                     /locus_tag="AC16JC_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR5163808.1"
                     /translation="MGRSHRCIGFLLCLGACGPAGRPAGPAPAALAHAESTYAATLTL
                     KDRLDILGWRGRRDFFPALRARYLAARGELVRALAIDSLALADTVDRRALAVMRGAVA
                     DALTEETESTDGPAVVDPARCVYEPHAVTDPDSLQSRLYDCYGAAARDVVVDGERLDR
                     LTVLGLLGVTGDPARRRRLFLGLDRVWRSVDGTDDRTSPWRELLRRRAHEWGTGPTPF
                     ARRVSELGFIPDSVEHWLVRLLEAWRATLPDSQLEPWDLYYAMGAASRRLSPRVPRDS
                     LLAVNGRFYRDLGADPVILGVHFDLEPRAGKDPVAFTTFGARPGFTRGAWQPGEPWVF
                     ASYAVGGLDNLGELLHETGHAIHIAGIRTRPAFSDWPDADLFTEAIADLADGEAYDGR
                     WQARYLGDSAPPVENRRAAYFGVMMDACWSLFEARMQRDPGLDPNVVWTELTSRYLRV
                     VPYPELSWWAMRGQLIDVPGYLVNYALGAFIAADLRARIRQVHGAFTLGDSTWYDFVR
                     ERIYRFGLERSSRRVLEEFLGRSLSAEALLADLQRGLNRGIDGPVK"
     gene            4228..>4935
                     /locus_tag="AC16JC_03580"
     CDS             4228..>4935
                     /locus_tag="AC16JC_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163809.1"
                     /translation="MADRRLTPVPEVPRHQRRWTPPTLTAGSAMALRAPLRGAIYVVL
                     IGITAAGLIYSLQGVIARNQRIQVRLAEMEGAINLLNAREWEARTGVISSDHRNDVAE
                     SQEHMRSILAELRERTGSQPKDAPSRGSSPQVTNLQQVESDVNRYLVAVDSMFDLLAR
                     VDKRAEAQTLAEQTVDPAFENLRLSLQGARSYYATAGERAIRLANIASLVGVLIAMSL
                     IWVLLGRSARQRAETQTL"
BASE COUNT          804 a         1748 c         1695 g          688 t
ORIGIN      
        1 cgaagtaaat cgtggccccc accgccgcgg cgcccatgtc caccgcctgc tgcaccgtgc
       61 cgaacaggat ctggtcgaac ttgttgggat aggtgagcag ctcgttgtgg ttgatcttca
      121 cgatgaaggg gatcttgtgc gcgtacttgc gcgccgcgac gccgagcacg ccgaacgtcg
      181 aggcgacggc gttgcagcca ccctcgatcg ccagcctgac gatgttctcg ggatcgaaat
      241 agtccgggtt cttggcgaag ctcgcgccgg ccgagtgctc gatgccctgg tccacgggga
      301 ggatcgagag atagcccgtc ccggcgagtc gcccggtccc gtacatgcgc tgcagattca
      361 cgagtacccg cgggctccgg tcgctcggcg cgaagatccg gtccacccaa tccggcccgg
      421 ggaggtgcag gcgctcgcgg gcgattttcg gcttgtcgaa cttcaggaaa tagtcggcct
      481 tgtcgccgag cgtcttcgtg atggcgtcga tcataaatcc tctgaggtga acggctcgtg
      541 ccgccgcaag attgtatcgg ccctgcggct caagcaagcc ccgcgccggg tacgcccgcc
      601 gggggcaatg gcgccttcca cccgtaatgc acggcgagca gtcgcagcac caccatgacg
      661 atcatggcga cgatgccggc gacggcgtgc gacaccatgg ccgactccca cagggcgacg
      721 aacagcgcgc tccccgtggc gctcaccgtg gcgtagagct cgccggtccg gaacagcgtc
      781 ggggcctccc ccgccagcac gtcgcgcagg atgccgcccc cgacggcggt cacgatgccc
      841 aggaacaccg ccgggacggg ggcgaggccg gcgccgagcg ccttgagcgc gcccaccacc
      901 gtatacacgg cgaggtacag cgcatcgatc gcgatcagga tgcggccgac gcgaaccacg
      961 gaaccgctgc ggtagaaccc caccgccgcc gcgaccacgg ccgtcagcag gtaccgggag
     1021 tcctgcagcg cggcgggcgg tccctgctgc agcagcacgt cgcggatgac cccgccgccc
     1081 acgcccgcca cgatcgcaag cgagacgcac cccacgagat cgaattgctt gcggacggcg
     1141 agcagagtgc cgctcaagcc gcccaccagc acggcgccga gatcgatgag cggggggacg
     1201 gagaagggca tcgggatgaa tgttcgccgc tggcggggcg gcacggaagc cggtctattt
     1261 cgcggcctca tggatcgcgc gcgcacgttt ctcgacgggc tgatcgcccg ctcgcaggtt
     1321 cccggtctcc agtacctggc gctggatcga gaccggaccc tgttcgagta cgcgggcggc
     1381 tgggccgaca tccgccaccg ggtcccgatg acggcggaca cgaccatgat ggcctattcg
     1441 atgagcaaga cgatcacggc ggtcgcggtg ctccagctcg tcgcggccca caaggtcagg
     1501 ttggacgatc cgatcgagcg ctaccacccc acaccctacg gaccgggcat cacggtgcgg
     1561 cacctcctcg cccacctgtc cgggatcccc aatcccatcc ccctgcggtg ggtgcatgcg
     1621 gccgcgcagc acgcgggctt cgacgagcac gcggcactcg aggcggtgct gcgggcccat
     1681 ccgcgggcgg cggccgctcc gggagcgaaa taccgctact cgaacatcgg gtactggctg
     1741 ctgggaagcg tggtcgagcg ggcgagcggc caatcgttcg cctcgtacgt ggccgagcgg
     1801 ggcttcgcgc cgctcgagat cgcgccggcg gcgctggggt acaccatccc cgatgcgggg
     1861 caccacgccc gcggatacct cgagaagtac tcgttcatga atctcgcgaa ccgcctgctc
     1921 atcgggcgcc aattcatcgg ggactacgaa ggggcgtggc tgcggatcga gccgcactac
     1981 ccgaacggac ccgcgttcgg tggcctggtg gggaccgcac gggggttcgg cgcctttctg
     2041 cgggaccaac tccgcccgcg atccgtactg ttcgacaatc ccgcccgcga gctgctgtac
     2101 acgccgcagc gcacgcgcgc gggccgcgtc gtccccatgg ggctcggctg gcacctgggg
     2161 gacctggacg gcattccctt tttctacaag gaaggcggcg gtggcggatt ccactgcgag
     2221 atgcgggtgt accgcgtcaa ccggatcgcg accgtcgtca tgaccagcgc cacgggcttc
     2281 gatgtgaaac ggtgtctcac ttcactggac cgtcaattcc tcggttgagg ccccgctgca
     2341 gatccgcgag cagcgcctcg gccgatagcg accgaccgag aaactcctcc aacacccgcc
     2401 gcgacgagcg ctccaggccg aaccgataga tccgttcccg gacgaagtcg taccaggtgg
     2461 aatcgcccag ggtgaacgcg ccgtgcacct ggcggatccg ggcccgcaag tcggcggcga
     2521 tgaacgcgcc aagcgcgtag ttgacgagat acccggggac gtcgatcagc tggccgcgca
     2581 tcgcccacca cgacagctcg gggtatggca cgacccgcag ataccggctc gtcagctccg
     2641 tccacacgac attgggatcg aggccggggt cacgctgcat ccgggcttcg aacagcgacc
     2701 agcacgcgtc catcatcacg ccgaaatagg cggcgcgccg gttctcgacg ggcggcgccg
     2761 aatcgcccag ataccgggcc tgccagcggc cgtcgtacgc ctcgccgtcc gccaggtcgg
     2821 cgatcgcctc ggtgaacagg tcggcatcgg gccagtcgct gaacgccgga cgcgtacgga
     2881 tgccggcgat gtgaatggcg tgtcccgtct cgtgcaacaa ctcacccagg ttgtcgagcc
     2941 cgcccaccgc gtaggaggcg aagacccagg gctcgcccgg ctgccaggcc ccgcgggtga
     3001 agccggggcg cgcgccgaac gtggtgaagg ccacgggatc cttgccggcg cgcggctcga
     3061 ggtcgaagtg cacgccgagt atcaccggat cggccccgag gtcgcgatag aaccgtccgt
     3121 tcaccgccag gagcgagtcg cgcgggaccc ggggggacag ccgccggctc gcggctccca
     3181 tcgcgtagta gagatcccag ggctccagct gggagtcggg cagggtcgcc cgccaggcct
     3241 cgagcagccg caccagccag tgttccaccg aatcggggat gaagcccagc tcgctcacgc
     3301 gccgtgcgaa cggcgtcggg ccggtccccc attcgtgcgc ccgtcgccgc agcagctcgc
     3361 gccacgggct cgtgcgatcg tcggtgccgt ccacactgcg ccacacccgg tcgagcccga
     3421 ggaacagccg ccggcgcctg gccgggtcgc ccgtgacgcc gagcaggccg aggaccgtga
     3481 gccggtcgag ccgctcgccg tccaccacca cgtcgcgagc cgccgcgccg taacagtcgt
     3541 acagcctcga ctgcagcgaa tcggggtcgg tcacggcgtg cggctcgtag acacagcggg
     3601 cgggatccac gaccgccggc ccgtccgtcg attccgtctc ctcggtcagc gcatccgcca
     3661 ccgcaccccg catcaccgcc agggcgcggc ggtccacggt atccgccagc gccagggagt
     3721 cgatcgcgag cgcgcgcacc agctcgccgc gcgccgccag atagcgggcc cggagggccg
     3781 ggaagaaatc gcgccgccca cgccagccga gaatgtccag ccggtccttg agggtcagcg
     3841 tcgcggcgta tgtcgactcg gcgtgggcca gcgccgccgg cgccggcccc gcagggcggc
     3901 ctgccggacc gcaggctcca agacatagga ggaatccgat gcagcgatgg gagcgaccca
     3961 tttgccggaa tgtcggcgtc ggggcagccg cgcggaaccc tgtccattag ttttgcgccc
     4021 atgatgcgac tcgacgcctc cgctgcgccg acccgcaccg gcgtccagac ggtcggcgtg
     4081 ccggtgggcg gcggtgccgt gttcgaggtc cgccgccatg ggcgtcctgc gtgccaggtg
     4141 atgattgagg cgtcgccgcc acaggcaggg aatcgccgga cgccggtagc cgaacgcagg
     4201 cgaccgagcg atcattgagg cacctgcatg gccgaccgtc gactcacccc ggttcctgaa
     4261 gtcccgagac accagcggcg ctggacgcct ccgaccctca ccgccggctc cgccatggcg
     4321 ctgcgggcgc cgttgcgcgg cgccatttac gtcgtgctca tcggcatcac cgccgccggt
     4381 ctcatctatt cgctgcaggg cgtgatcgcc cggaaccagc gcatccaggt gcgcctggcc
     4441 gagatggaag gggcgatcaa cctgctcaat gcgcgggagt gggaagcgcg caccggcgtg
     4501 atcagctcgg atcaccgcaa cgacgtcgcg gagagccagg agcacatgcg ctcgatcctc
     4561 gccgagctgc gtgagcgcac cgggagccag ccgaaggacg cgccctccag gggctccagc
     4621 ccgcaggtga cgaacctgca gcaggtcgag tcggacgtga accggtacct ggtcgcggtc
     4681 gattcgatgt tcgacctgct ggctcgcgtc gacaagcggg ccgaggcgca gaccctggcg
     4741 gagcagaccg tggatccggc gttcgagaac ctgcgcctgt cgctgcaagg ggcgcggagc
     4801 tactacgcca ccgccgggga gcgggctatc cggctggcga acatcgcctc cctggtgggc
     4861 gtgctgatcg ccatgagcct gatctgggtc ctcctggggc gcagcgcccg gcagcgtgcc
     4921 gagacccaga cgctg
//
LOCUS       DBNGZW010000147         8700 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_78079, whole genome shotgun sequence.
ACCESSION   DBNGZW010000147 DBNGZW010000000
VERSION     DBNGZW010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8700)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8700)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8700
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_78079"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..948
                     /locus_tag="AC16JC_03585"
     CDS             <1..948
                     /locus_tag="AC16JC_03585"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR5163810.1"
                     /translation="LDGVIVYCNQRFADIVRTPLARVIGSALHRFVPPAGQATLAALL
                     EPASRGRTEGELVFRAGAGTSVPVSVSFAPLRLAPGAEVSGVIGVVVDATERKQQETL
                     RSRLIEQVMTAQDAERGRFARELHDETGQALTALLVGLRTIEEARTTAEAVELAQRLR
                     EVAARTLRDVGRLARGLHPSVLDDLGFAVAVTRHVQEFARVHGIAEETRIEGLDASPP
                     SPLVQTTVYRVLQEALTNVAKHAGARRVRVHLTCDGTAVALRVQDDGDGFEPGAAGRR
                     GLGLRSMQERAALLGGAVQVASAQGEGTTISARFPLRPS"
     gene            954..1607
                     /locus_tag="AC16JC_03590"
     CDS             954..1607
                     /locus_tag="AC16JC_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5163811.1"
                     /translation="MSSIRVLIADDHAVLRAGLRMLIEAQSDMIVVGEAADGPETVSR
                     ARALGSDVVLLDLSMPGPPATVTIERLVRLAPSPRILVLTMHDDPVYLRSALEAGASG
                     YIVKKAADVELLNAIRIVHRGGSVVNLTRPGESTGSDEVRRVRRDSPAAGEPKPLSRR
                     EVEVLRLLAQGHTNQEAADRLSVSVKTIETHRKRLSDKLGLRSRAELFRYAVESRLL"
     gene            1796..2449
                     /locus_tag="AC16JC_03595"
     CDS             1796..2449
                     /locus_tag="AC16JC_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR5163812.1"
                     /translation="MLIIDDNRLVREGLRVLLDEQTDLKVVATAEDRATGLRHVAEST
                     PHIVLVDAALDNGDSFEFVGLLRETAPEVKVIVMDLLPAPEDVIEFVKAGANGFIMKD
                     ATVQDLVSTIRSVALGADVVPPALTGTLLSHIAQQAAAVRQVPGASDAVRMTKREKEI
                     MNLIAEGLSNKEIAQHLNIATYTVKSHVHNILEKLALHSRLQIAAYTHRAGPLSAAT"
     gene            complement(2473..2832)
                     /locus_tag="AC16JC_03600"
     CDS             complement(2473..2832)
                     /locus_tag="AC16JC_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163813.1"
                     /translation="MVTKGQRRRVPGRWVYAYELNPPQPEPRFRKIRVLLRRAQAAAR
                     RGGRLWTGRIVVESNITHILVVTDDPGQVRHVDRAIEAELQHLNMQFALTGPARFTLP
                     RVTDGKRVPKRKRAPKS"
     gene            3074..3373
                     /locus_tag="AC16JC_03605"
     CDS             3074..3373
                     /locus_tag="AC16JC_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163814.1"
                     /translation="MSRSGCVWAYAYRITPPQPKHRLETIQALLDHEHGDALQEARRW
                     TGRLVLERRATRILIVSDSPDQERAVDHRLAGEVRRLQATLTRTVPLEIAADPVD"
     gene            complement(3458..3736)
                     /locus_tag="AC16JC_03610"
     CDS             complement(3458..3736)
                     /locus_tag="AC16JC_03610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163815.1"
                     /translation="MPCILAILILLTPRLAVALLWYFTHWFDGLFPSLLLPIVGFLFL
                     PTTLLWYSAVHHWFGGAWTTGPVVGLVVALLIDLSALGGHRRFTRRRS"
     gene            4123..5616
                     /locus_tag="AC16JC_03615"
     CDS             4123..5616
                     /locus_tag="AC16JC_03615"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR5163816.1"
                     /translation="MSRTSWFTRGRSLGLLLFFAGIDASGCARNPATGSNQLMLVSES
                     QEIAMGRQADTAVIATIGLYPDAVWQRYIQQFGARLAATSERPNLPWTFRVVDDPAVN
                     AFALPGGFVYVTRGLLTHLTSEAELASVVGHEIGHVTARHTAAEMSKQELIGLGLAVG
                     SMANSQVAKYAGTANQALGILYLKFSRDDESQADQLGLRYMRRANFDPRQMPEVFRML
                     DRLSAAEGGARLPTWLETHPSPANRVAAINSQIAALPQDFSGTSVNRDAYERRLEGLV
                     FGQNPRQGYFVGSQFSHPDLAFRVTFPDGWATNNGAQAVVAVSPQGDAAVELSQARAQ
                     NADAAARAFLSQQGITGGTAARTTQSGLSGVSAPFAAATTNGELRGRVTFVEYGGGVY
                     QLVGYATAARWPAYQAAAERTLGSFQRLTDPAALNAQPQHVSIVPISQRTTIDALLQQ
                     RASPVSAETLALINQVERQTPLDAGRLVKWVIGQPLPTSTSARATGQ"
     gene            5688..6311
                     /locus_tag="AC16JC_03620"
     CDS             5688..6311
                     /locus_tag="AC16JC_03620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163817.1"
                     /translation="MVAALSLATLVACKPTGQAAETTTSTKTPAARATRTLDAGRHFT
                     MATTETISSKTSKPGDSFTATVVADVTDDAGRVAIPAGSVVSGTITDVKSAPNPTSPG
                     TLTLAVNSVNVRGRSYPIEASIDSLDTERQGQGVTGGDVAKVGAGAAAGAILGRVIGK
                     NTKGAVIGGIMGGIVGAGIAVQGKDANIVLPAGTHVVVTLGTHLTVT"
     gene            6308..6529
                     /locus_tag="AC16JC_03625"
     CDS             6308..6529
                     /locus_tag="AC16JC_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163818.1"
                     /translation="MKPQVIAGIVIAGLGAYVLAKGLTYRSQSNVLKVGDVQVTAEEH
                     RLVPPWVGGVAVVGGLVLIGAGVRGRRGA"
     gene            6639..6887
                     /locus_tag="AC16JC_03630"
     CDS             6639..6887
                     /locus_tag="AC16JC_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163819.1"
                     /translation="MNLARRCARVIAALQAAGADEALVRFIQPIDLAIHAAQPAEVTD
                     DERPAHRAPRAAMPGGEPARPGAAPPFALGVARRTIGG"
     gene            7178..7363
                     /locus_tag="AC16JC_03635"
     CDS             7178..7363
                     /locus_tag="AC16JC_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163820.1"
                     /translation="MRPTRLIPPDSGPIEDLDAPAYTRALTPLPEIQRRLAMQAANRE
                     EESNRVFNREFLLRSQL"
     gene            7725..>8700
                     /locus_tag="AC16JC_03640"
     CDS             7725..>8700
                     /locus_tag="AC16JC_03640"
                     /inference="COORDINATES: protein motif:HMM:NF025104.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HIR5163821.1"
                     /translation="MTTGEWEIARALPGLRWTWPFALPARTRSSFVARALRVSDLVAG
                     LVALAGALVVVNLDALPHGIAAFLSLRITVDKFLLLLVVAVCWNRTFRWLGLYRRSHT
                     GAPREELARVATAVSLGALPSLLFPLFSRTGSFTLQTAALFWALSIPIVLGGRAVVRA
                     VGSSPSARRAREVLIVGSGPRAEALCRMLRGPEVAGASLVGFVDSKDRTRVRGGGDLP
                     PCLGSLGDLESLLMRRVVDEVLIALPIKSCYERIQEVIHTCERLGVQSTYLADIFEPS
                     LGRVRYEQPAFLTVRVVQDDFLLIIKRAIDLVGAALGLVVLAPLLLLIA"
BASE COUNT         1338 a         2925 c         3056 g         1381 t
ORIGIN      
        1 ttggatggcg tcatcgtcta ctgcaaccag cgtttcgccg acatcgtccg gacgccgctc
       61 gcgcgcgtca tcggctcggc gctgcaccgg ttcgtaccgc ccgccgggca ggcgacgctc
      121 gcggcgctgc tcgaaccggc gagccggggc cggacggagg gcgagctcgt gttccgcgcg
      181 ggcgcgggta cgtcggtgcc ggtgtcggtc agcttcgcgc cgttgcggtt ggcgccaggc
      241 gcggaggtca gcggggtgat cggcgtggtc gtggatgcca ccgagcggaa gcagcaggag
      301 acgctccgga gtcggctgat cgagcaggtg atgaccgccc aggacgcgga gcgggggcgg
      361 ttcgcccgtg agctgcatga cgagaccggg caggcgctga cggccctcct ggtggggctg
      421 cgcacgatcg aggaggcccg gaccacagcg gaagcggtcg aattggcgca acgactgagg
      481 gaggtcgccg cccgcaccct gcgggatgtg ggacgcctgg cccgggggct ccacccgagc
      541 gtcctcgacg atctggggtt cgccgtggcg gtgacccgcc acgtgcagga gttcgcccgg
      601 gtgcacggga tcgcggagga gacgcggatc gaggggctcg atgcgagccc cccgtcgccg
      661 ctggtgcaga ccaccgtgta ccgggtgctg caggaagcgc tgaccaacgt cgcgaagcac
      721 gccggtgcgc ggcgcgtgcg cgtacacctg acgtgcgacg ggacggcggt ggcgctgcgc
      781 gtgcaggatg atggcgacgg gttcgagccg ggcgccgccg gtcgccgagg cctcggtctc
      841 cgcagcatgc aggagcgggc ggcgctgctc ggcggcgcgg tgcaggtcgc ctcggcgcag
      901 ggggagggga ccacgatctc ggcccgcttt ccgttgcgac cctcgtagcg cgcatgtcga
      961 gcatccgcgt gctgatcgcc gacgaccacg cggtccttcg ggccggcctt cggatgctga
     1021 tcgaggccca gtccgacatg attgtcgtcg gcgaggccgc ggacggcccc gagacggtaa
     1081 gccgggctcg agccctgggg tccgacgtcg tgttgctgga cctctccatg cccggccccc
     1141 cggccaccgt gaccatcgag cggctggtcc gcctggcgcc gagcccccgg atcctcgtcc
     1201 tcaccatgca cgacgatccg gtctacctgc ggtccgcgct cgaggctggc gcatccggct
     1261 acatcgtcaa gaaggcggcg gacgtcgagt tgttgaacgc gatccgcatc gtccatcgcg
     1321 gcggcagcgt cgtgaacctg acgcgtcccg gggaatcaac gggcagcgac gaggtccggc
     1381 gcgtgcgccg cgactcgccg gccgccggcg aacccaagcc gttgagtcgc cgcgaggtcg
     1441 aggtgctccg gctgctggca caagggcata cgaatcagga ggcggcggac cggctgtccg
     1501 tgagcgtcaa gaccatcgaa acccaccgca agcgactgag cgacaaactc ggcctcagga
     1561 gccgcgcgga gctgttccgc tacgccgtcg agtcacgcct gctgtagtat cggggtttct
     1621 cccgacaccc tcttcgcggt tggcctgttt ccccgatagc cgccgaccct atcgtgcccc
     1681 acctttgcat ccggggtctc aggccttggg tgagcggcgt caccggctcc ggggttgctc
     1741 gcaggatcgc cccattaacc tgaacgcgga cacggctctg ggaaaacgca tccgggtgtt
     1801 gatcatcgac gacaatcgac tcgtccgcga agggctcagg gtcctgctcg acgaacagac
     1861 ggacctgaaa gtcgtggcga ccgcggaaga ccgggcgacc gggctgcgcc acgtggcgga
     1921 gtccacgccg cacatcgtgc tggtagacgc cgcgttggac aacggcgaca gtttcgagtt
     1981 cgtgggactg ctccgcgaga ccgcgccgga ggtcaaggtg atcgtgatgg atctgctgcc
     2041 ggcgcccgaa gacgtgatcg aattcgtcaa ggccggggcg aacggcttca tcatgaaaga
     2101 cgcgacggtc caggatctcg tgagcacgat tcgatcggtc gcgctggggg cggatgtggt
     2161 gccgccggcg ttgaccggca ccctgctgtc ccacatcgcg cagcaggcgg cggcggttcg
     2221 tcaggtgccg ggtgcgtcgg acgccgtgcg aatgacgaag cgtgagaagg agatcatgaa
     2281 cctgattgcc gagggcctga gcaacaagga gatcgcccag cacctcaaca tcgccaccta
     2341 cacagtcaag agccacgtgc acaacattct ggagaagctg gcgctgcaca gccggttgca
     2401 gatcgccgcc tacacccacc gggctgggcc cctctccgcc gcgacgtagc tcccctcggc
     2461 tgatcccgcc gttcagctct tcggagcgcg ctttctcttc ggaacacgct ttccgtccgt
     2521 cacgcgcggc agcgtaaacc gcgcgggccc agtgagggcg aattgcatgt tgagatgctg
     2581 cagctcggcc tcgatcgccc gatcaacgtg ccgtacctgg ccggggtcgt ccgtcacgac
     2641 aaggatgtgg gtgatgttgg actccaccac gatccggccg gtccacagcc gtcccccacg
     2701 gcgcgccgcc gcctgtgcgc ggcgcagcag cacccggatc ttcctgaagc gcggctcggg
     2761 ctgcggggga ttgagctcgt aggcgtagac ccagcgcccc ggcacccgcc gtcgctgccc
     2821 cttggtaacc ataagacctt cctctctcct cttcagccaa acaagctacc cagcgggcgc
     2881 acggcagcgc catcgctctt gtgggtgacg cccggcgcgc tcgatgtacg acgactcggc
     2941 gatccgtgcc gctgagtcgt tcctggaccc aaatggctag accgttccac ccgctgtagc
     3001 taagccgccg ttcattcgat ggggggatgt cggcgtgctg cgggccccgg caggtttggc
     3061 ttggagaact gccatgtcga gaagtggatg tgtgtgggcc tacgcctatc ggatcacccc
     3121 gccacagccg aagcaccgcc tggagacgat ccaagcgctc ctggaccacg aacatggcga
     3181 cgcgctgcaa gaggctcgcc gctggaccgg tcggctggtg ctggaacggc gggccacgcg
     3241 cattctgatc gtgagcgaca gccccgacca ggagcgcgcg gtcgaccacc ggttggcggg
     3301 tgaggtgcgg cggctgcagg cgacgctcac tcgcacggtg ccgttggaga ttgcggcgga
     3361 cccagtcgac tgacgggagg caccgttatc gccacgcgaa cggaccgcct catgggcgcg
     3421 gcggtccgcg ccgcgcgccg ccgccgccgg tcttcgttca ggaccgccgg cgcgtgaacc
     3481 tgcggtgccc accgagcgcg gagagatcga tcaggagcgc caccacgagg cccacgaccg
     3541 gcccggtcgt ccacgcgccg ccgaaccagt ggtgcaccgc cgaataccag agcagggtcg
     3601 tgggcagaaa gaggaagccc acgatgggca acagcagcga ggggaagagg ccgtcgaacc
     3661 aatgggtgaa ataccagagg agggcgaccg cgagccgcgg tgtcaggagg atgaggatgg
     3721 cgaggatgca gggcatggcg cgctcccgag tgctcagttg tccgatctgc tgccggcgtc
     3781 cgtgtacatc gcgatctcca cgcgccggtt ctgccgccgg tcgtcatccg agtcgttcgt
     3841 gacgatcggc tcggttgcgc cgcgaggatg atacacgacg tggtggagaa tgccccggag
     3901 tacggccgcc ccggtatcgc ccggacggat ggaaatggaa agagagtgga accccgttct
     3961 gccagtggtg tccgtccaag gagctaagtc gttgttcatc ccgctgagtg atgttgcgca
     4021 gcggcccgta cgtgcaggtt gtggttgagg atagccgtcg ttcccagccg gccagcgtca
     4081 tcgctggtcc ggcgcttgag acatcccggg agtactcgtc caatgtcacg aacatcttgg
     4141 ttcacccgcg gccggtccct cggcctgctg ttgttcttcg ccggcatcga cgcgagcggc
     4201 tgcgcccgca atccggctac cggctccaat cagctcatgc tggtcagcga gtctcaggag
     4261 atcgcgatgg gccgtcaggc cgacaccgcg gtcatcgcca ccatcggact ctatcccgat
     4321 gcggtctggc aacgctacat ccagcagttc ggtgcgcggc tcgccgcgac ttccgagcgc
     4381 cccaacctgc cgtggacgtt ccgcgtggtg gacgacccgg cggtgaacgc cttcgccctc
     4441 cccggcggtt tcgtctacgt gacgcgggga ctcctgaccc atctcacctc cgaggcggag
     4501 ctggcctccg tcgtcggtca cgagatcggc cacgtcacgg cccggcacac ggccgccgag
     4561 atgagcaagc aggagctcat cgggctgggt ctggccgtgg gcagcatggc gaattcccag
     4621 gtggcgaaat acgcgggcac cgcgaaccag gcgctcggga tcctgtatct caagttctcc
     4681 cgcgacgacg agagccaggc ggatcagctc ggcctgcgct acatgcgccg cgccaacttc
     4741 gatccgcgcc agatgcctga ggtgttccgc atgctcgacc ggctcagcgc ggccgagggc
     4801 ggcgcccgtc tccccacgtg gctcgagacg caccccagtc cggcgaaccg ggtcgccgcc
     4861 atcaactctc agatcgcggc gctgccgcag gatttctcgg ggacgagcgt caaccgcgac
     4921 gcttacgagc ggcggctgga gggcctggtg ttcgggcaga atccacgcca gggctacttc
     4981 gtgggtagcc agttctcgca tcccgatctg gcgttccggg tgacgttccc cgatggctgg
     5041 gccacgaaca acggggcgca ggcggtggtc gcggtcagcc cgcagggcga tgccgcggtc
     5101 gagctgtcgc aggcgcgggc gcagaatgcc gacgcggcgg cccgggcctt cctgtcgcaa
     5161 caggggatca cgggcggcac cgccgcgcgc acgacccaga gcgggctgtc cggcgtgagc
     5221 gcgccctttg cggccgccac tacgaacggc gagctccggg gcagggtcac cttcgtggag
     5281 tacggtggcg gcgtctatca actcgtgggc tacgctacgg ccgcgcgttg gccggcctac
     5341 caggcggcgg cggagcgcac gctcggaagc ttccagcgac tcaccgatcc cgcggcgctg
     5401 aacgcgcagc cgcaacacgt cagcatcgtg ccgatcagcc agcggaccac gatcgacgcg
     5461 ctcttgcagc aacgggcatc gcccgtgtcc gccgagacgc tcgccctcat caaccaggtc
     5521 gagcggcaga ccccgctcga cgcggggcgg ctcgtgaaat gggtcatcgg acaaccactt
     5581 ccgacatcga cctccgcgcg cgcgaccggt cagtgatcgc gcggcggaga cagcagattc
     5641 tcgcaggact ctggagatgc actatgaaac ccaattactg gaccagcgtg gtggcggcct
     5701 tgagccttgc cacccttgtc gcctgcaagc caaccgggca ggcggccgaa accacaacct
     5761 cgacgaaaac cccggcagcc cgggccaccc gcacgctcga cgccgggcgg cacttcacca
     5821 tggctacgac ggagactatc agctccaaga cttcgaagcc gggcgattcg ttcaccgcga
     5881 cggtcgtggc ggacgtcacc gatgacgcgg gccgggtggc catcccggca ggctccgtgg
     5941 tgtccgggac gatcaccgac gtcaagtccg cgccgaaccc gaccagcccg ggcacgctga
     6001 ccttggcggt gaacagcgtg aatgtccgcg gccggagcta tccgatcgag gccagcatcg
     6061 attcgctgga taccgagcgt caggggcagg gcgtcacagg gggcgacgtg gccaaggtgg
     6121 gcgcgggcgc ggccgcgggt gcgatcctgg gccgggtcat cggcaagaac accaaaggtg
     6181 cggtgatcgg cgggatcatg ggcggtatcg tcggcgccgg catcgcggtg cagggaaagg
     6241 acgcgaacat cgttctcccg gccgggaccc acgtggtggt cacgctgggg acccacctca
     6301 ccgtcacatg aagccccagg tcattgccgg catcgtgatc gccggtctcg gggcctacgt
     6361 gctggcgaaa gggctcacgt accgatccca gagcaacgtt ctgaaagtcg gcgacgtgca
     6421 ggtcaccgcc gaggaacata ggctggtgcc cccgtgggtc ggcggggtcg cggtcgtcgg
     6481 cggattggtc ctgatcggcg ccggcgttcg ggggcgtcgt ggagcgtaac acgtcgaggc
     6541 gggttccagc gaagcggagc gtggatgttc tcgcggcgac ggtcgcccgg acgctcggta
     6601 cttctcgtcg ggctgcagaa gttgcactgt gggggccgat gaacctggcc cgccggtgcg
     6661 cccgcgtgat tgccgcgctc caggcggccg gcgccgacga ggcgctggtg cggttcattc
     6721 agccgatcga cctcgcgatc catgccgctc agcccgctga agtcacggac gacgagcggc
     6781 cagcgcaccg cgctccccgt gcggccatgc ccggcggtga accggcccga cccggggcgg
     6841 cgccgccgtt cgccctcggg gtcgccagga ggacaattgg cggatgagat gcctgacttg
     6901 aggcccggcg ccgcggtgtg cgcgcggtgc ggcgcatacg gcgatcacct gccgtgtcac
     6961 gcggggcgcg tcggcgaccg ggagcggatc ctgtggtttt gcgcgcggtg cctcgatccc
     7021 caccacgaca cgcctgggct gtccggcggg tagtggttgc aggccgctgg agagaggtcc
     7081 cgatggtaac cgttctggtg ttgctgctgt tcagcctgtc gctgctgcag agtgaagacg
     7141 tctggcgcac cgacgtggag cgaccgggag gagtccgatg aggcccacac gactgatacc
     7201 gcccgatagc ggcccgatcg aagatctcga cgccccggcc tatacgcggg ccctgacgcc
     7261 gcttcccgag atccagcggc gcctggcgat gcaggcggcc aatcgggaag aagagagcaa
     7321 tcgcgtgttc aaccgggagt tcctgctccg cagtcagctg taggcccagc tgtaatcctt
     7381 cgctgaaccg cttttccgtg cactgaaagg cagcagggga gaaccgcgtt acccgggttc
     7441 ctgtgacgag ccccacgctc gccgtggtac acaacttctc cggcgcctgg cgcgggcccg
     7501 agcgtttcgc atcgcatcgg tcgagcgcgg accttcctcc acggatctcg gtagcacggg
     7561 catgtcgtcg atgcagtcgg tgcagcacgc ctgtggcgcg cccttcaccg acctccacca
     7621 gtcctctcgt cgtgatcctc ggaggccgat gcgctccacc ggcaatcgta cggacgaatc
     7681 cgtggcatga tggcgacgcg atcggttgcg tgcgcgaggt gcgcatgacg acgggtgagt
     7741 gggagatcgc ccgggcgctc cccggtttgc ggtggacctg gccgttcgcc ctccccgcgc
     7801 ggactcggtc gtcgttcgtc gcccgcgcgc tcagggtgag cgatctggtg gccgggctcg
     7861 tggcgctggc cggcgcgctc gtcgtggtca acctcgacgc cctgccacac ggcatcgcgg
     7921 cgtttctctc cctgcggatc acggtcgaca agttcctgct gctgctcgtg gtcgccgtat
     7981 gctggaatcg cacctttcgc tggctgggcc tgtaccgccg atcccacacc ggcgcccccc
     8041 gcgaggagct ggcgcgtgtg gcgaccgccg tgtcgctggg cgccttgccg tcgctgctgt
     8101 tccctctgtt cagcaggacg ggatcgttca cactccagac cgcggcgctg ttctgggcgc
     8161 tgtccatccc gatcgtactg ggcgggcggg ccgtcgttcg cgccgtcggg agctcaccgt
     8221 cggcgcggcg ggcccgtgaa gtgctgatcg tcggcagcgg ccccagagcc gaagcgctgt
     8281 gccggatgct gcgcggcccc gaagtggcgg gcgcgtcctt ggtcggcttc gtggactcga
     8341 aggaccgcac ccgcgtccga gggggaggcg acctgccgcc gtgccttggc tcgctcggcg
     8401 atctggaatc gctgctgatg cgccgggtgg tggacgaggt gctcatcgcc ctgccgatca
     8461 aatcgtgcta cgagcggatt caggaggtca tccacacctg cgagcgactg ggcgtccagt
     8521 ccacctacct cgcggacatc ttcgagccgt cactggggcg ggtgcggtac gagcagccgg
     8581 ccttcctcac ggtcagggtg gtgcaggacg acttcctgct gatcatcaag cgggccatcg
     8641 acctcgtggg ggcggcgctc ggcctcgtgg tgctggcccc gctgctcctc ctgatcgccg
//
LOCUS       DBNGZW010000148         2387 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1513362, whole genome shotgun sequence.
ACCESSION   DBNGZW010000148 DBNGZW010000000
VERSION     DBNGZW010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1513362"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..828
                     /locus_tag="AC16JC_03645"
     CDS             <1..828
                     /locus_tag="AC16JC_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0065002 - intracellular protein
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HIR5163822.1"
                     /translation="GVFVMWLAEQITERGLGNGQSLMIFFSIVERIGPETVRTFEALQ
                     AGSLRFGVLIVILAIMVLVVAGTVAVTVAARRIPIQIPRKVMGRGRIAEGRKTFIPLR
                     INSSGVMPIIFAQSLIIVPGTLATFSNVPWLKQFANYFSVTSPVYLVSLFLLIVFFAY
                     FYTSIIFNPVDLAENLKKQGGFIPGVKPGAATADYIDEVLSRITLPGALFLTVVALLP
                     IVIGNVLNMPFGFGGTALLIVVGVALDTVQQVQQHLLLRHYDGFMKEGRIKFRGRTR"
     gene            854..1417
                     /locus_tag="AC16JC_03650"
     CDS             854..1417
                     /locus_tag="AC16JC_03650"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014510789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HIR5163823.1"
                     /translation="MFILLLGAPGSGKGTQGAILAQRLGIPKIATGDIIRAAMKGGTP
                     LGVQAKTFYDAGKLVPDTLILELIKSELAKPEAHDGAIFDGFPRTAAQAELVEKSLTS
                     RGQRLAHVLLLDVPEEELVKRLHGRALQEGRADDTPEAIRTRLLTYHKDTAPLVAYYA
                     QRGVMHRVPGVGTVEEIAEEIKRILGR"
     gene            1414..2187
                     /gene="map"
                     /locus_tag="AC16JC_03655"
     CDS             1414..2187
                     /gene="map"
                     /locus_tag="AC16JC_03655"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013824042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HIR5163824.1"
                     /translation="MITLKSPREIETMAAAGRIVAETLALMGRTVKAGISTKELDRRA
                     EEFIRSHPGALPSFKGLYDFPATLCTSINQEVVHGIPSAKRVLKDGDLISVDVGVQLE
                     GLHADSAATFPVGEVTPDVRRLLQVTQEALAAGIAQAKAGNHVGDIGHAVQQVAEAAG
                     YSVVREMVGHGIGSSFHEEPQVPNFGKPKRGPRLVPGMTIAIEPMVNVGGAEIRTLSD
                     KWTVVTVDGSLSAHFEHTVAIGDNGAAPRILTQLSKNGH"
     gene            complement(2184..>2387)
                     /locus_tag="AC16JC_03660"
     CDS             complement(2184..>2387)
                     /locus_tag="AC16JC_03660"
                     /inference="COORDINATES: protein motif:HMM:NF016825.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclodeaminase/cyclohydrolase family protein"
                     /protein_id="HIR5163825.1"
                     /translation="VTLAREIGGIGNKNARSDALVGGGLARAALAGAVENVRVNVESL
                     GDPTPGAALLEEAQRLEREAVPS"
BASE COUNT          385 a          794 c          833 g          375 t
ORIGIN      
        1 ggcgtgttcg tcatgtggct ggccgagcag atcaccgagc gcggcctggg caacgggcag
       61 agcctgatga tcttcttctc gatcgtggag cgcatcgggc ccgaaaccgt ccgcaccttc
      121 gaggcgctgc aggcgggatc gctgcgcttc ggcgtgctga tcgtgatcct cgcgatcatg
      181 gtgctggtgg tggcgggcac ggtcgcggtg acggtggccg cgcggcgcat cccgatccag
      241 atcccccgca aggtgatggg tcgaggacgc atcgccgagg gacgcaagac gttcatcccg
      301 ctgcgcatca actcgtccgg cgtgatgccg atcatcttcg cccagtcgct catcatcgtc
      361 ccggggacgc tggcgacgtt cagcaacgtg ccgtggctga agcagttcgc gaactacttc
      421 tcggtgacct cgccggtgta cctggtgtcg ctgttcctgc tgatcgtgtt cttcgcctac
      481 ttctacacgt ccatcatctt caacccggtg gatctggcgg agaacctcaa gaagcagggc
      541 gggttcattc cgggagtgaa gccgggcgcc gcgaccgccg actacatcga cgaggtgctg
      601 tcgcgcatca cgctgccggg cgcgctgttc ctcaccgtgg tggcgctgct gccgatcgtc
      661 atcggcaacg tgctcaacat gccgttcggc ttcggcggca cggcgctgct gatcgtcgtc
      721 ggcgtggccc tcgatacggt gcagcaggtg cagcagcatc tgctgctgcg ccactacgac
      781 gggttcatga aggaaggccg catcaagttc cgcggccgca cccgctaagg cgggggtgcg
      841 ggggagggcg gcgatgttca tcctgctgct gggcgcgccc ggttcgggca aggggacgca
      901 gggcgcgatt ctggcgcaac gcctcggcat accgaagatc gccactggtg acatcatccg
      961 cgcagcgatg aagggcggga cgcccctggg cgtgcaggcc aagacgttct acgacgccgg
     1021 caagctcgtt cccgacaccc tgatcctcga gctgatcaag agcgagctgg cgaagcccga
     1081 ggcgcacgac ggggcgatct tcgacggctt tccccgtacg gcggcgcaag cggagctggt
     1141 ggaaaagagc ctcacgtcgc ggggccagcg gctcgcccac gtcctgctgc tcgacgtccc
     1201 ggaagaggag ctggtcaagc gcctgcacgg ccgcgcgctg caggaaggcc gtgccgacga
     1261 tacccccgag gcgatccgca cccggctgct cacctaccac aaggacaccg ccccgctcgt
     1321 agcctactat gcgcagcgcg gcgtgatgca ccgggtgccg ggcgtcggca cagtcgagga
     1381 gatcgccgag gaaatcaagc ggattctggg acgatgatca ccctcaagtc gccgcgcgag
     1441 atcgagacca tggcggccgc cggccgcatc gtcgcggaga cgctggccct catggggcgc
     1501 accgtgaagg ccggcatctc gacgaaagag ctggaccgcc gggccgagga gttcatccgc
     1561 agccaccccg gcgcgctgcc gtcgttcaag gggctgtacg acttccccgc cacactctgc
     1621 acctcgatca atcaggaagt ggtccacggc atcccgtccg ccaagcgcgt cctgaaggac
     1681 ggcgatctga tcagcgtgga cgtgggcgtg cagctcgagg ggctgcacgc cgattcggcg
     1741 gcgaccttcc ccgtgggcga ggtgacgccc gacgtccggc gtctgctgca ggtgacccag
     1801 gaggcgctcg cggcggggat cgcgcaggcg aaggcgggca accacgtggg cgacatcggg
     1861 catgcggtgc agcaggtggc ggaagcggcg ggctactcgg tggtgcggga gatggtgggg
     1921 cacggcatcg ggtcgagctt ccacgaggag ccgcaggtgc cgaacttcgg caagccgaag
     1981 cgggggccgc ggctcgtccc cgggatgacg atcgccatcg agccgatggt gaacgtgggc
     2041 ggggcggaga tccggacgct gtccgacaag tggaccgtgg tgacggtgga cggctccttg
     2101 tcggcacact tcgagcacac ggtggcgatc ggggacaacg gggcggcgcc gcggatcctc
     2161 acccagctgt cgaagaacgg gcactagctc ggcacagctt cccgctccaa ccgctgagcc
     2221 tcctccaaca acgccgcccc cggggtcgga tcgcccagcg attcgacgtt gacccgcacg
     2281 ttctccaccg ctcccgcgag cgcggcgcgg gccaatcctc ctcccaccag cgcgtccgag
     2341 cgcgcgttct tgttcccgat gccgccgatc tcccgcgcga gggtcac
//
LOCUS       DBNGZW010000149         2167 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1782029, whole genome shotgun sequence.
ACCESSION   DBNGZW010000149 DBNGZW010000000
VERSION     DBNGZW010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1782029"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(256..>2167)
                     /locus_tag="AC16JC_03665"
     CDS             complement(256..>2167)
                     /locus_tag="AC16JC_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163826.1"
                     /translation="AGEAGALAAADAERAHLAVAEAYAPQALPLRAASLVRRGRRRVV
                     MGIGAVALALVSAAVGFLYAPTFGGAPGYQQGTIYLASGGASSPSHRLRWPARRGRLA
                     SIEVVRGAQSRQPSPLFTRPVTAMNETHNKVFAIRGTDTAQVTFGRSDDVSPMWAPDG
                     GRFLFVRGWRASETRYQQNVFLADSSGRIERPITDTRWQDQSPAWSTAGTQIAFARDS
                     ASVWSIWVADGDGTNAWNVTEEMGLPRRDGRPSFAPDDRRLAVLYPDTGSSRGALYVV
                     DLADRLATPLRGTIVNAVEEPAVWSPDGRWLAYRARHGDARRLWVVSAEEPMVPALVA
                     ELPLDLSPHDWRAGKRRFVSKVELLPAAVTLRTSRGVRVIARVLTPDGEAPSSVLRWS
                     AEDTMVASVDDLGFVRGRHAGSTRLVASAGGVRADTAMVTVTAAPIDTLFDEDWTGGF
                     DTTRWIPFGFPHSVVVSGAPPDGRPAFFNNGDYNHGSGAVSVQLFEVGDEGLTLETEA
                     WLPFTGQHWQIWQIALADDPFASGPEISGGPLAIGVSGPEPTAPAAHWMCAEAVMPTA
                     PRVHAGHWVRIALVVRPDGWSECRVDGRRFSARQVPKGLLERPLAIVLRGHSVGTRIY
                     HGRVVVTRGLPY"
BASE COUNT          391 a          822 c          637 g          317 t
ORIGIN      
        1 tgaaccacgc tccgaatgtc aaccaccacc gtcacaccct cgacacaccg ataggggaac
       61 cggccccaac ctcccttcgc cttgtggcgg aaggccggat cgcggagtgg aatgcacgac
      121 gcgcggcggt caccatccgg cctcattgca tgcggagagt agcctgcgtc acacgccgtc
      181 acacgacacg caaaagagcc ggctctccgc cggctcctgt tgcacgccca cctcatctcg
      241 tcattgtccg tcgcgtcagt acggcaaccc cctggtcacc accacccttc cgtgatagat
      301 ccgcgtcccc acggaatgac cccggagcac gatcgccagc ggccgctcga gcaacccctt
      361 cggcacctgt ctcgccgaga acctccgccc gtccacccgg cactcgctcc agccgtcagg
      421 ccgcaccacg agcgcgatgc ggacccagtg tcccgcgtgc acccgcggtg cagtaggcat
      481 taccgcctca gcgcacatcc agtgagcagc gggggcggtg ggctccggcc ccgaaacccc
      541 aatcgccaac ggccccccac tgatctcagg gccactggcg aacgggtcgt cggccaacgc
      601 gatttgccag atctgccagt gctgccccgt gaacgggagc cacgcctcgg tctcgagggt
      661 caacccttcg tcacccacct cgaacagctg caccgatacc gcgccgctgc catggttgta
      721 gtccccgttg ttgaagaacg ccggccgccc atcgggcggc gccccgctca ccacaacgga
      781 gtgcggaaag ccgaacggga tccaacgcgt agtgtcgaat cctcccgtcc agtcctcgtc
      841 gaacagcgta tcgatcggcg ctgcggtgac ggtcaccatc gcggtgtccg cccggacccc
      901 acccgcactg gccaccaagc gggtcgagcc tgcatggcgg ccccgcacaa acccaagatc
      961 gtccacgctc gctaccatgg tgtcctcggc agaccagcgg aggaccgagc tgggagcctc
     1021 accgtctggg gtgaggaccc tggcgatgac tcgcacgcct cgactggtgc gcagcgtgac
     1081 ggcggcaggt agcagctcca ccttcgacac gaagcgccgc ttcccggccc gccaatcgtg
     1141 aggagataga tcaagcggga gctcggcgac aagcgcaggg accatcggct cctcagcaga
     1201 gaccacccac aatcgacgtg cgtctccgtg ccgcgcacga taggccagcc atcgcccgtc
     1261 cggcgaccac acggcgggtt cctcgacggc gttcacaatc gtaccgcgaa gcggtgtcgc
     1321 tagtcggtca gccagatcca cgacatacag agccccccgg ctcgagcccg tgtccgggta
     1381 cagtactgcg agtcgtcgat cgtcaggcgc gaaagacggc cgtccgtcgc gacgcgggag
     1441 tcccatttcc tccgtgacat tccaggcatt ggtgccgtcc ccgtccgcca cccagatgct
     1501 ccacacagaa gccgaatccc gcgcgaatgc gatctgcgtc ccggcagtag accatgccgg
     1561 agactgatcc tgccaacgcg tgtccgtgat cggacgctcg atgcgtcccg atgagtccgc
     1621 gaggaacaca ttttgctggt acctcgtctc actggccctc cagccgcgta caaacaggaa
     1681 acggccgcca tccggggccc acatcggaga aacatcgtca ctccggccga acgtgacctg
     1741 cgcggtgtcg gtgccccgga tcgcaaacac cttgttgtgg gtctcgttca tcgcggtgac
     1801 gggcctcgtg aacagcgggc tcggctgtct agattgggca ccgcggacta cctcgatgct
     1861 cgcaagtcga cctctcctcg cgggccagcg aagccggtga cttgggctcg aagcgccacc
     1921 gctcgcgaga tagatcgtgc cctgctggta accaggcgcg ccaccgaacg tcggcgcgta
     1981 caggaaccca acggccgccg aaacgagcgc tagagcgacg gccccgattc ccatcacgac
     2041 ccgccgccgg ccacgccgca ccaggctcgc cgcccgcagc ggcagcgcct gcggcgcata
     2101 cgcctcggcc accgccagat gcgcgcgctc ggcgtccgcc gccgctagtg ccccggcttc
     2161 gcccgcc
//
LOCUS       DBNGZW010000150         4866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1240492, whole genome shotgun sequence.
ACCESSION   DBNGZW010000150 DBNGZW010000000
VERSION     DBNGZW010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1240492"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="AC16JC_03670"
     CDS             <1..264
                     /locus_tag="AC16JC_03670"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014716.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR5163827.1"
                     /translation="AALPGLVDQLSRILDYVAQIAGVAASESAQPFVPGPAAIRFRPD
                     EVRPAPLAFGPDRMAPQFRDGFFLVPRLGAFDPGSANGAEEDG"
     gene            261..1625
                     /gene="gatA"
                     /locus_tag="AC16JC_03675"
     CDS             261..1625
                     /gene="gatA"
                     /locus_tag="AC16JC_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011606923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR5163828.1"
                     /translation="MTIAETVDRLERTAGLNALLTPIAEVRSALASQAATAPAEGPLA
                     GMPVAVKDNISTLEYTTSCGSRILEGYRSPFEATAITRLKAAGALIAGKTNCDEFAMG
                     SSTEHSAFGRTLHPLDPARVPGGSSGGSAALVAAGAVPAALGSETGGSVRQPAAFCGV
                     VGIKPTYGRVSRYGLVAFGSSLDQIGVCGRTVSDAGRVLAAISGRDPLDSTCEDRDPL
                     PLPAVPENLQGFVVGIPKEYFPPDLDPGVRRACDRLIRLLKDLGAAVRDVSLPHTAYA
                     IPTYYIVAPAEASSNLARYDGVRFGPRFDGAMDVRALYRSTRGRGFGAEVRRRILVGT
                     YVLSSGYYDAYYRRAQRMRALIAQDFRNVFDRGVDLLFTPSTPTPAFTAGEKLGDPVA
                     MYLADIFTVTANLAGRSALTLPIGRVNGLPVGGQLIGPPFLEDEMLEAAFTLERAVAA
                     EAEA"
     gene            1622..3061
                     /gene="gatB"
                     /locus_tag="AC16JC_03680"
     CDS             1622..3061
                     /gene="gatB"
                     /locus_tag="AC16JC_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR5163829.1"
                     /translation="MSAVDRWETVIGLETHVQLRTRSKMFCGCSSVFGAPPNINVCPV
                     CLGLPGALPTVNEQAIRLAVRAALALGCRVQPHSVFARKNYFYPDLPKGYQISQYEEP
                     LATDGALAFASPERGTVTATIVRLHVEEDAGKSLHDRVPGATAVDLNRSGVPLAEIVT
                     GPDFRSPQEARAYLLALKEVLQYADVSDCDLEKGSLRVDANLSVRPAGHPELGTKTEV
                     KNINSFAFVEKALGLERDRQVATLLAGGRIEQQTMLFDAKANAVRPQRSKEESKDYRY
                     FPEPDLPPLVVPDEWLLEERAILPELPAARRQRFATQYGLPAQDAIVLVQSREIADYF
                     EATVAAGAEPKPAANWVMGEVLADAKNRGGVLRVTPDRLAQLVALVKDGTVSNNAAKQ
                     VFAALADNGSGDPRTTAERLGLRLVADEDALTGWVDQVLAAHPAEVERYRKGETKLLS
                     FFVGQVMKASKGKADPTQVTRLLKERLGG"
     gene            complement(3039..3956)
                     /locus_tag="AC16JC_03685"
     CDS             complement(3039..3956)
                     /locus_tag="AC16JC_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013078.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HIR5163830.1"
                     /translation="MALVAIVVFPGTAHAWTPATHIYLGESVLANLGTLPTAVADLLR
                     AFPYDFLYGNIAADTSMAKKYAPVGRHCHAWHVGREIYELAHTDGLRAFGLGYLSHLA
                     ADTVAHNFYVPRQLVLTSSTAALGHSYWESRLETHLGDRYARTAKSVILQDHGVSDTH
                     LDAILSPTLFSIRTNRRLFRGMVHLTESQSWQWAFQIIAENSRWDLPGEDVERHMAVA
                     FEYVMEGLAESNAATHRLDPAGEKALGLAKQMRRRALQEGGRGDPGRVRATAEEHFGL
                     PRSSLAFWRDAKAAKPWREAEAISPPAAP"
     gene            complement(3980..4402)
                     /locus_tag="AC16JC_03690"
     CDS             complement(3980..4402)
                     /locus_tag="AC16JC_03690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163831.1"
                     /translation="MIPTWAAVLGALSLAVIALAALVSAAAVLAAAFGVRSFLSAVRH
                     TAEPVVNDVRALIGTIRDEADGLAETSRDLRQRIVHAADAAEARLADLGETLEVAERE
                     VSETAKDVAATARTVRRGVSVWRLGRALKRRSDKREDD"
     gene            complement(4399..>4866)
                     /locus_tag="AC16JC_03695"
     CDS             complement(4399..>4866)
                     /locus_tag="AC16JC_03695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163832.1"
                     /translation="AGVGLAAGIGLATGTSPLLVCALTGAALARWSSAHSRLALDIGA
                     TEPVAAAALWVAAGALIRGPVLVVALAAILLALVPLVRQRFLPRDAAGRTLGLAVALS
                     FTFTAAGPLGPSAGAVRTAAALALLLTGLPTLLRGAGPGGPLTSRHRNAEVSG"
BASE COUNT          710 a         1703 c         1766 g          687 t
ORIGIN      
        1 gcggcgctgc cgggcctggt ggaccagttg tcgcgcatcc tggattacgt ggcccagatc
       61 gcgggcgtcg ccgccagcga gtcggcgcaa ccgttcgtgc ccgggcccgc ggcgatccgg
      121 ttccggcccg acgaggtgcg gcccgcgccg ctcgcctttg gtcccgaccg gatggcgccg
      181 caattccgcg acggattctt cctggtgccg cgtctcggag cgttcgatcc gggcagcgcg
      241 aacggcgccg aggaggacgg gtgacgatcg cggaaacggt cgaccgcctg gaacggacgg
      301 cggggctgaa cgcgctgctc acaccgatcg ccgaagtgcg gagcgcgctc gcctcccagg
      361 ccgcgacggc gccggcggag gggccgttgg ccgggatgcc ggtcgccgtg aaggacaaca
      421 tctccacgct cgagtacacc accagctgcg gctcccgcat cctcgagggc taccgctcgc
      481 cgttcgaggc gaccgcgatc actcgcctga aggcggcggg cgcgctcatc gccggcaaga
      541 cgaattgcga cgagttcgcg atgggctcct cgaccgagca ttccgccttc gggcgcacgc
      601 tccatcccct cgatccggcg cgcgtgcccg gcggctcgtc gggcggctcg gcggcgctgg
      661 tcgccgccgg cgcggtcccg gccgcgctcg gctcggagac cggcgggtcg gtgcgccagc
      721 ccgccgcgtt ctgcggcgtc gtcgggatca agcccaccta cggccgggtg tcgcgctacg
      781 ggctggtggc gttcggcagc tcgctcgatc agatcggcgt gtgcgggcgg accgtgtcgg
      841 atgccggccg ggtgctcgcc gcgatcagcg gtcgggaccc gctcgactcc acctgtgagg
      901 accgcgaccc gctcccgctg cccgcggtgc cggagaacct gcaggggttc gtcgtcggca
      961 tcccgaagga gtacttcccc cccgatctcg atcccggcgt gcggcgcgcg tgcgaccggc
     1021 tcatccggct gctgaaagac ctcggggcgg ccgtacggga cgtgtcgctg ccgcacacgg
     1081 cgtatgccat acccacttac tacatcgtcg cgccggccga ggcctcgtcc aacctcgcgc
     1141 gctacgacgg cgtccgcttc gggccccgat tcgacggcgc gatggacgtg cgtgcgctgt
     1201 accgttcgac ccgtggccgg ggattcggcg ccgaggtccg ccgccgcatc ctggtgggca
     1261 cctatgtgct gtcgtccggc tactacgatg cctattaccg ccgggcgcag cggatgcgcg
     1321 cgctcatcgc gcaggacttc cgcaacgtct tcgatcgcgg ggtggacctg ctgttcacgc
     1381 cgtccacacc cacgccggcg ttcacggcgg gtgagaagct cggggatccg gtggcgatgt
     1441 atctggccga catcttcacg gtgaccgcga acctcgccgg ccggtcggcg ctgacgctgc
     1501 ccatcggccg cgtgaacggg ctgccggtgg gcggccagct catcgggccg ccctttctcg
     1561 aagacgagat gctggaagcg gccttcacgc tcgagcgggc cgtcgcggcg gaggcggagg
     1621 cgtgagcgcg gtggatcgct gggagaccgt gatcggtctc gagacgcacg tccagctgcg
     1681 cacccggtcg aagatgttct gcggctgctc cagcgtgttc ggggcgccgc ccaacatcaa
     1741 cgtgtgtccc gtctgcctcg ggctcccggg cgcgctcccg acggtcaacg agcaggcgat
     1801 ccggctcgcc gtgcgcgccg cgctggcgct cggctgccgc gtgcagccgc acagcgtctt
     1861 cgcccgcaag aactacttct atcccgacct gcccaagggc tatcagatct cgcaatacga
     1921 ggagcccctg gcgaccgacg gcgcgctcgc cttcgccagt cccgagcgcg gcacggtgac
     1981 ggccaccatc gtgcggctcc acgtcgagga agacgcgggc aagtcgctcc acgaccgcgt
     2041 acccggcgcg acggccgtag acctgaaccg ctcgggcgtg ccgctggccg agatcgtgac
     2101 cggcccggac ttccgctcgc cgcaggaggc ccgggcctat ctgctggcgc tgaaggaagt
     2161 gctccagtac gccgacgtgt ccgactgcga cctggagaag gggagcctgc gggtggacgc
     2221 gaacctgtcg gtgcgccccg ccggccatcc cgagctcggc accaagaccg aagtgaagaa
     2281 catcaactcg ttcgcctttg tcgaaaaggc gcttggcctg gagcgcgatc gccaggtcgc
     2341 gaccctcctg gcgggcgggc ggatcgaaca gcagacgatg ctgttcgacg ccaaggcgaa
     2401 cgccgtgcgg ccgcaacgct cgaaagagga gagcaaggac taccggtatt tccccgagcc
     2461 cgatctgccc ccgctcgtcg tgccggacga atggttgtta gaagagcggg ccatcctccc
     2521 ggagctgccc gcggcccggc ggcaacgctt cgccacgcag tacggattgc cggcgcagga
     2581 tgccatcgtg ctggtgcagt cgcgcgagat cgcggattac ttcgaggcga cggtggcggc
     2641 cggcgccgag cccaagcccg cggccaactg ggtcatgggg gaggtcctcg cggacgcgaa
     2701 gaaccgcggt ggcgtgctgc gcgtcacccc cgaccgcctg gctcagctgg tcgctctggt
     2761 caaggacggg acggtgtcga acaacgcagc gaaacaggta ttcgccgcgc tggcggacaa
     2821 cggcagcggc gatccccgca ctacggccga gcggctcggg ctacggctgg tggccgatga
     2881 ggacgccctg accggctggg tggatcaggt gctggccgcg caccccgcgg aagtcgagcg
     2941 ctaccgtaag ggggagacga agctgctgtc gttcttcgtg gggcaggtca tgaaggcgtc
     3001 gaagggaaag gccgacccga cgcaggtcac ccggctgctc aaggagcggc tggggggctg
     3061 atcgcctccg cttcgcgcca cggcttggcg gctttggcgt cccgccagaa cgcgagcgag
     3121 gagcgcggca ggccgaaatg ttcctcggcg gtcgcgcgca cccgcccggg gtcgccacgg
     3181 cccccttcct gcagcgcgcg gcggcgcatc tgcttcgcca gcccgagcgc cttctccccg
     3241 gccgggtcga ggcgatgggt cgccgcgttg gattcggcga ggccctccat cacgtattcg
     3301 aacgccaccg ccatgtggcg ctccacgtcc tcgcccggca gatcccaccg gctgttttcg
     3361 gcgatgatct gaaacgccca ctgccagctc tggctctcgg tcaggtgcac catgccccgg
     3421 aacagccggc ggttggtccg gatgctgaac agggtgggcg agaggatggc gtcgaggtgt
     3481 gtgtcgctca ccccatggtc ctggaggatc acggacttgg ccgtgcgtgc gtagcggtct
     3541 cccaggtggg tctcgagccg gctctcccag tagctgtgcc ccagcgccgc ggtactgctc
     3601 gtgaggacca gctggcgcgg gacgtagaag ttgtgggcca cggtgtcggc ggccagatgg
     3661 gacagatacc cgagaccgaa ggcgcggagt ccgtcggtgt gcgccagctc gtagatctcg
     3721 cggccgacgt gccacgcgtg gcaatgacgc cccacggggg cgtacttctt cgccatcgag
     3781 gtatccgcgg caatgttgcc gtagagaaaa tcgtagggaa aggcccgcag caggtcggcg
     3841 accgccgtgg gcagcgtgcc gagattggcg aggaccgact cgccgaggta gatgtgggtc
     3901 gccggggtcc aggcgtgggc ggtgccgggg aacaccacga tcgcgacgag tgccacgacg
     3961 aaaagcgcca gcgcgcgctt cagtcgtcct cccgcttgtc ggagcgccgc ttcagggcgc
     4021 ggccgagccg ccagaccgac acgccgcggc gcaccgttcg agccgtggca gccacgtcct
     4081 tggccgtctc gctgacttcg cgttcggcca cctccagtgt ctcccccagg tcggcgagcc
     4141 gcgcttcggc cgcgtcggcc gcgtgcacga tgcgctgacg gagatcgcgg gaggtctcgg
     4201 ctaacccgtc tgcctcgtcg cgaatcgtgc cgatgagggc gcggacgtcg ttgacgaccg
     4261 gctccgccgt atggcgcacg gcactcaaga aggagcgaac cccgaacgcg gcggccagca
     4321 cggcggccgc cgaaacgagc gcggccagcg cgatgaccgc aaggctcagg gccccgagga
     4381 cggcggccca cgtcgggatc atcctgaaac ctcggcgttc cggtggcgtg aagtcaacgg
     4441 gcccccgggg cccgccccac gcagcagcgt gggcagccca gtcaggagca gggcgagcgc
     4501 cgcggccgtc cggaccgccc cggcactcgg cccgagcggg ccggcggcgg tgaacgtgaa
     4561 gctcagagcg accgcgaggc cgagcgtacg tccggccgcg tcccgcggaa ggaaccgctg
     4621 ccgcacgagc ggcacgagcg cgagcaggat ggcggccagg gccacgacga gaacaggacc
     4681 acggatcagc gcgccggcgg cgacccacag cgccgcggcc gccacgggct cggtggcgcc
     4741 gatgtcgagc gcgagccggg agtgcgcgga cgaccagcgc gccagcgcgg cgccggtgag
     4801 cgcgcacacg aggagcggcg aggttccggt tgccaatccg atgccggcgg cgaggccgac
     4861 gccggc
//
LOCUS       DBNGZW010000151         4479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1105845, whole genome shotgun sequence.
ACCESSION   DBNGZW010000151 DBNGZW010000000
VERSION     DBNGZW010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4479
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1105845"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(17..2719)
                     /locus_tag="AC16JC_03700"
     CDS             complement(17..2719)
                     /locus_tag="AC16JC_03700"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5163833.1"
                     /translation="MATFFEQFRDFLAPRFQLEHELGSGGMGTVYRARDTALPRFVAV
                     KVLPPSKAGATAIARFLREARILARLDHPNIVKIIDVGCDSDPFIYYIMDYFDGGTLA
                     DRLTKGPLSPEEAATFGRHILDALRHAHSRGVIHRDIKPSNIFWGTGRGWVLADFGIA
                     KSSDEAGEPYTEPGQVAGTPGYIPPERQVRQDLPQGDLYSVGLVLYQALTGEHWNVET
                     EPEQTDWTGVPAQMRPVLRRALARAPEDRWPDAAAFQRALWRRRSDARSWARAGSLLA
                     ASIVVAGVIWFLLHPKVDLNIGRVETGAGVPTGLGDSLPALITRQLTGYPDFSVGGWR
                     ARTTVTGTATLSGSTLQLRLRFPGQPAATISTTSEQWRTAAGMLADSLLVRLYGSSRL
                     DFDLSVGVLPREPEGLRSFLVAEKAFARADWVSAYDGYHDASGVDRSCALCFWRHAEV
                     ARFLAYPPDTADATEYRARVNEFPLNYQTLIRAELVPLDWRLDSLYALAQRAPRFLFG
                     PFRWGDELLHRGPLVGQPRLGASVGLSRATEIQHDLAPAWEHLAWLWIAEGDRRRAEA
                     ALDTLSVLPPPDPRGAAMRDLLHVAYAWRFDPARAQAGTAAVLAAAALRADEPLDAGA
                     RYLNHFAAPEGAVWLGEQLEARGYRPISAMLAQAFGNLSAGRPGRAHQVLMRAVTQFG
                     DARLELLDDEIEAVRLMFDVDSLAAATSWVPVDTRLRDLSQRVPGSLRARAAWTRALL
                     ARRFGNAQPAVAADVPVPLKQLLAVYDVASRGRYTEALEQSRQLLGLGAEFQGDPFLH
                     TMLHLLRAEWRVRAGTPQLADQELLWYENSDPVGLPQDDPQSMEVDWAFGTLARWWRV
                     RLATSEATRCRLAREVARLWTGAEPSYAARADNARAIVRSCPAS"
     gene            complement(2791..3939)
                     /locus_tag="AC16JC_03705"
     CDS             complement(2791..3939)
                     /locus_tag="AC16JC_03705"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR5163834.1"
                     /translation="MRKLCALATFVALALGCGRDQLGPTPRFDANVVEVPDATGNPGI
                     TVMTYNVYYGTDPSPLLTAPLDQVPFVAAQVWGKVIQTDFPARAGALAALIARRQPHL
                     VGLQEAALYRIQSPGDAVIGGSVPATTVTYDFLALLVDSLAARGLTYVLAASDSTTDV
                     EVPVFTGVDGSGNPTFDDVRLTDRDAVLARADVEVTNPQHGKYQAHIPVSFGPVETGV
                     YEGWASVDATFQQRTYRFVSTHLEAQLAVPVQIAQAQELLGLLQSETRPTILVGDFNS
                     DVLGHVPEKATPSYGMITDAGFSDSWVRPPQDVLGATCCESDDLTNPEPALNQRVDFI
                     FTRYMPDLVAPIQHEVVGDRARDRTPGGLWPSDHAGVFQAFVIPALVP"
     gene            4067..4456
                     /locus_tag="AC16JC_03710"
     CDS             4067..4456
                     /locus_tag="AC16JC_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR5163835.1"
                     /translation="MVAPARSQLSRRERQIMDIIYRLGRATAAEVHDALPDRPSYSTI
                     RALLRVLEAKGHLRHVEDGPRYVYSPTVPRERARESALRQVVTTFFDGSTEAAVAALL
                     DISAARLSDTELNRLAGLIAQAKKEGR"
BASE COUNT          737 a         1615 c         1470 g          657 t
ORIGIN      
        1 agcaggctgg cgtcgatcat gaggccggac agctccgcac gatcgcccgc gcattgtcgg
       61 cccgggcggc gtaggacggt tccgcccccg tccacagtct cgccacctcc cgcgccagcc
      121 tgcaccgggt cgcttccgaa gtggccagcc gcacgcgcca ccatcgtgcc agtgttccga
      181 aggcccagtc cacttccatc gactgcggat cgtcttgcgg gaggcccact ggatcggagt
      241 tctcgtacca gagcagttcc tgatcggcaa gctgcggcgt gcccgcgcgc acccgccact
      301 cggcgcgcag cagatgcagc atggtgtgca ggaacgggtc gccctgaaat tcggccccga
      361 gccccaggag ctgccggctc tgctcgagcg cctcggtgta ccgcccgcgc gacgcgacat
      421 cgtacactgc gagcaattgc ttcagcggga ccggaacgtc cgcggccacg gccggctggg
      481 cattcccgaa gcgccgggcc aacaacgctc gcgtccacgc cgcccgcgcc cgaagactcc
      541 cggggacccg ctgcgagagg tcccgcagac gtgtatccac cggcacccag ctcgtcgccg
      601 ccgcgaggga atccacgtcg aacatgagtc gcactgcctc gatctcatcg tccagcagct
      661 cgagtctggc atcgccgaac tgggtgaccg ccctcatcag gacctggtgc gccctgccgg
      721 gtctgcctgc actgaggttc ccgaatgcct gggccagcat cgccgaaatc gggcggtagc
      781 cacgtgcctc cagttgttcc ccgagccaca cggcaccctc tggagcggcg aagtggttga
      841 ggtagcgcgc cccggcatcg aggggctcgt cagcgcgcag ggcggccgca gccaggacgg
      901 ccgcggtgcc ggcttgcgcc cgcgccgggt cgaaccgcca ggcataggcg acatgcagca
      961 ggtcccgcat cgcggctccc ctggggtccg gcggcggcag gacggacagc gtgtcgagcg
     1021 ccgcctcggc acgccgccgg tccccttccg cgatccacag ccaggcgagg tgctcccagg
     1081 cgggggcgag atcgtgctgg atctcggtcg ccctgctgag ccccaccgag gcgccgagcc
     1141 gtggctgccc gaccagcggc ccgcggtgca gcagctcatc gccccaccga aacgggccga
     1201 acaggaacct cggagcacgc tgggccagcg cgtacagcga gtcgagccgc caatccagcg
     1261 gcaccagctc ggcgcgaatc aacgtctggt agttcagcgg aaactcgttg acgcgcgcgc
     1321 gatactcggt cgcgtcggcg gtatccggcg ggtaggcgag aaaccgtgcc acctccgcgt
     1381 gacgccagaa gcacagggcg cagctgcgat ccactccgga ggcgtcgtga tacccgtcgt
     1441 acgccgacac ccagtccgcc cgggcgaacg ccttctccgc caccaggaac gaccgcagtc
     1501 cctccggctc ccgcggcagc acgccaaccg acagatcgaa atcgaggcgg ctcgacccgt
     1561 acagccgcac cagcaggctg tcggccaaca tccccgcagc ggtacgccac tgttcactcg
     1621 tggtcgagat cgttgccgcc ggttgcccgg gaaaccggag gcggagctgg agcgtggatc
     1681 ccgacagcgt cgccgtgccg gtcaccgtgg tccgggcgcg ccagccgccg acggagaagt
     1741 cggggtatcc cgtgagctgc cgggtgatga gtgccggcag cgagtccccg agccccgtcg
     1801 gcacccccgc acccgtctcc acccgcccga tgttcagatc cactttcggg tgcagcagaa
     1861 accagatcac gcccgccacg acgatcgatg cggccagcag cgatccggcc cgcgcccacg
     1921 accgcgcgtc cgaccgccgc cgccacagtg cccgttgaaa cgccgcggca tcgggccagc
     1981 ggtcctcggg agcccgggcg agggcacgtc gcagaacggg ccgcatctgc gccggcacgc
     2041 cggtccagtc ggtctgctcc ggctccgtct ccacgttcca gtgctcgccc gtcagcgcct
     2101 ggtacagtac cagtcccacc gagtacagat cgccctgcgg gagatcctgc cggacctgtc
     2161 gctccggcgg gatgtatccc ggcgtgccgg ctacctgccc gggctccgtg tatggctcac
     2221 ccgcttcgtc cgacgacttg gcgatcccga agtccgcgag cacccacccc cggccggttc
     2281 cccagaagat gttgctcggc ttgatgtccc ggtgaatcac tccgcgggag tgcgcatgac
     2341 gcagcgcatc caggatgtgc cggccgaacg tggccgcttc ttccggcgag agcgggccct
     2401 tcgtcaggcg gtcggcgagc gttccaccat cgaagtagtc catgatgtaa tagatgaatg
     2461 gatcggagtc gcagccgaca tcgatgatct tcacgatgtt cgggtgatcc agtctcgcca
     2521 ggatgcgggc ttcacgcaga aaccgggcga tcgccgtcgc gccggccttc gaggggggca
     2581 acaccttcac cgcgacgaat cgcggcagcg ccgtgtcgcg cgcccggtac acggtgccca
     2641 tgccgccgct gcccagctcg tgctccagct ggaaacgcgg tgccaggaag tcgcggaact
     2701 gctcgaagaa ggtggccatg gccggcggcc acgccaatgt agcattggaa gccgccggca
     2761 ccagttgacc atggaatcag ccgcaccgca tcacgggacc agcgccggaa tcacgaacgc
     2821 ctggaacact cccgcatgat cggaaggcca gagcccgccc ggcgtgcgat ctctcgcccg
     2881 atcccccacg acctcgtgct ggatcggagc cacgagatcc ggcatatacc gggtgaagat
     2941 gaagtcgacc cgctggttca acgccggctc gggattggtg aggtcgtcac tctcgcagca
     3001 ggtcgcgccc agcacgtcct gaggcggacg cacccagctg tcgctgaatc cggcgtcggt
     3061 gatcatcccg taggacggcg ttgccttctc gggaacgtga cccagcacgt ccgagttgaa
     3121 atcacctacg aggatggtcg gccgggtctc cgactggagc aggcccagga gctcctgggc
     3181 ttgcgcgatc tgcaccggca ccgccagctg cgcctcgaga tgggtcgaca cgaaccggta
     3241 ggtgcgctgc tggaacgtgg cgtccaccga tgcccagccc tcgtatactc ccgtctccac
     3301 cggaccgaag ctcaccggga tgtgggcctg gtacttcccg tgctgcgggt tggtgacctc
     3361 cacgtcggcg cgggccagca ccgcgtcccg gtccgtcaac cgcacgtcgt cgaacgtcgg
     3421 gttgccgctg ccgtccacgc cggtgaagac cggaacctcg acgtcggtgg tggagtcgga
     3481 ggccgcgagg acgtaggtca ggccgcgcgc ggcgagcgag tcgaccagca gggcgagaaa
     3541 gtcgtaggtg accgtcgtcg caggaaccga gccgccgatc acggcgtccc cgggcgactg
     3601 gatcctatac agcgcggcct cctgcagtcc cacgagatgc ggctggcgcc gcgcgatgag
     3661 cgccgccagc gcccccgccc gcgccgggaa atcggtctgg atcaccttcc cccagacctg
     3721 cgcggcgacg aacggcacct ggtcgagcgg cgccgtgagc agcgggctgg ggtcggtacc
     3781 gtaatagacg ttgtacgtca tgaccgtgat gccggggttg ccggtggcgt cgggtacctc
     3841 gacgacattg gcgtcgaagc gcggcgtggg tccgagttga tcgcgaccgc agccgagcgc
     3901 cagggcgacg aacgtggcga gcgcgcagag cttccgcatg actacccccc tgaaatgagt
     3961 gacccggggg aagttcgggc ctcggcgtca agaatcggtc aagccgggct tgctaagttt
     4021 tcagcactat gctagaattt tagcacttcg aacccgggag tcgtgcatgg tcgcccccgc
     4081 ccgctcgcag ctgtcgcggc gcgaacgtca gatcatggac atcatctacc ggctgggccg
     4141 cgccactgcc gccgaggtcc acgatgccct cccggaccgg cccagttact ccaccatccg
     4201 cgcgctgctg cgcgtgctcg aggccaaggg tcacctgcgc cacgtggagg acggcccccg
     4261 ctatgtgtac tcgcctaccg tgccccggga gcgcgcccgt gaatcggcgt tacgccaggt
     4321 ggtcaccaca ttcttcgacg ggtccaccga agccgccgta gcggcactgc tcgacatctc
     4381 cgccgcacgt ctctccgaca ccgagctgaa ccgactcgcc gggctcatcg cccaggccaa
     4441 gaaagaaggg cggtgaccca tgcccgactt cgcgctcga
//
LOCUS       DBNGZW010000152         4761 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_78679, whole genome shotgun sequence.
ACCESSION   DBNGZW010000152 DBNGZW010000000
VERSION     DBNGZW010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4761)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4761)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4761
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_78679"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..844)
                     /locus_tag="AC16JC_03715"
     CDS             complement(56..844)
                     /locus_tag="AC16JC_03715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163836.1"
                     /translation="MTTSLQQATVRLERLFSGVDTRPGVIARRLLGIPKPEDDRLAEL
                     LCIEHRARSKMDGSIGGSFVTTAWAAWEMMDLGFDALQGSLSRLISWVLTFLESPPPA
                     PAPTPLALPNGTVLTNEAGATFAAECLGLRVLLRARQDARPRVLRRLERVLSLWPTVH
                     DELAASALGVVAVSPVPYHDRLESCVERIGAAQHSDGTWGDANLFHMLESLLLAGIRP
                     ARDVITRAIPALLALQQDDGSFDSPAHEERALIGLRAIRWAGVG"
     gene            complement(1000..1335)
                     /locus_tag="AC16JC_03720"
     CDS             complement(1000..1335)
                     /locus_tag="AC16JC_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163837.1"
                     /translation="MTLGRLVLAWLPVAAWFCLAGWTANRLVGGGVVANAPVTTSVFV
                     ALAVEALLVTLFGSLWFDSLGHGGWWLLFGLVGILSTRVPTRPSFVSIVHAVVRYVGA
                     GALLAWRLG"
     gene            complement(1332..3053)
                     /locus_tag="AC16JC_03725"
     CDS             complement(1332..3053)
                     /locus_tag="AC16JC_03725"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047420 - N-acyl-D-amino-acid deacylase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl-D-amino-acid deacylase family protein"
                     /protein_id="HIR5163838.1"
                     /translation="MRRLPPLALAALALGVACNRPAGRGPRPMGGAAYDVLLQGGWVV
                     DGTGNPRWRGDVAIRGDRIAALGRLSDVAARETLDVSGMIVAPGFIDMMGQSEINVLI
                     DNRVLSKVTQGVTTEITGEGGSVAPLTDALVVPDSDAMRKYRYREDWRDFDGYFAQLA
                     RNGSTVNLATFVGATQVRLAVIGSEDRQPTAAELAHMAALVDTAMVQGALGVWSALEY
                     APASYSTTEELIALARAARRHGGIYASHMRNEGSRIDQALDEVFRIAREADIPAEISH
                     LKVAGRRSWGRMPAVLARIDSARRAGLDVTADQYPYIAAATSLDASLPTWAESGGLDS
                     LLAHLRDPATRARIHGEMLRPAGGIEGFYNEAGGGAGILITGVFEDSLRGLQGKRISE
                     IAAARNRDPIETIFDILLAEGPHRTDAVYFDMSEPDVQAAMKTWWVAVNTDFGGVAPD
                     GPLGGQSAHPRAYGTFPRILGRYVRDSGLMTLEFAVRKMTSLAAQRVGLVDRGLLRPG
                     MYADITVFDPATVADRATFDKPHQPSVGIEYVLVNGQTVLKRGELTAARPGRGLRGPG
                     YVPPGAR"
     gene            complement(3050..4411)
                     /locus_tag="AC16JC_03730"
     CDS             complement(3050..4411)
                     /locus_tag="AC16JC_03730"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5163839.1"
                     /translation="MRWWRAAVRVAATALAAACTSPGKGMIALEGATLIDGSGGPPKA
                     DALILIKDGTIRAVAQVNEIKVPRGAQRIDLVGKTIMPGLIDAHAEVERWALPRYLAW
                     GVTSVRAVHSVGDSGYAIKKDIDLGSVVGPRLYTAGPVIDGLAATLPGGTAVAADEEA
                     RQAVDQRAVAGADFIMAYTKITPELLRAIVDEATTLRLQVSARPGKMDALTAARSGVA
                     SLEQLAGVVSAAAGDPAPFLRANDLFFAGLTMEEASWAALDSGAVARLAAALAATRVA
                     IVPTLVEHETLARLDDASLVQRLGMADVPKEGDNPVRAVSALLQRAGWRAADIAAFRR
                     GRARQDQFVREFRAAGGLVAAGTDAANPLLVPGASLHEEMALLVAAGMTPMDAITSAT
                     RYGAQVLAADSLGMLSPGKVADLVVLNRNPSEDITATRDIAWVMVRGRQFRPDSLRTS
                     WSK"
     gene            complement(4408..>4761)
                     /locus_tag="AC16JC_03735"
     CDS             complement(4408..>4761)
                     /locus_tag="AC16JC_03735"
                     /inference="COORDINATES: protein motif:HMM:NF025887.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR5163840.1"
                     /translation="ARSAADWNRGDPDGFMGDYVKDSTVGYVSGGHVQYGWQPLYDHY
                     KATYFAPGKSRDSLSFVEIAVRALTTDLAYATARFELHRGDSLVASGPFTLILQKRGD
                     RWQILHDHTSADPKP"
BASE COUNT          728 a         1675 c         1698 g          660 t
ORIGIN      
        1 gagtgttgac cggaagttac acctgccccg gtgccgggaa cacgtcaggc ggccatcagc
       61 ccaccccggc ccagcggatc gcgcggagcc cgatcagcgc ccgctcctcg tgcgccgggc
      121 tgtcgaagct gccgtcgtct tgctgcagcg cgagcagcgc cgggatcgcc cgcgtgatca
      181 cgtcgcgcgc cgggcggatg ccggcgagca gcagcgattc gagcatgtgg aacaggttgg
      241 catcgcccca ggtgccgtcg gagtgctgcg ccgcgccgat gcgctcgaca cacgattcga
      301 ggcggtcgtg atagggcacc ggcgacacgg cgaccacccc gagcgcgctg gcggcgagct
      361 cgtcgtgcac ggtgggccag aggctcagaa cgcgctccag ccggcgcagc acccgcggcc
      421 gggcgtcctg gcgcgcccgg agcagcacgc gcaggccgag acactccgcc gcgaacgtgg
      481 caccggcttc gttcgtgagc accgtgccgt tgggcagggc gagcggcgtg ggcgccggag
      541 ccgggggagg cgattcgagg aaggtcagga cccaggagat gaggcgcgac aggctcccct
      601 gcagcgcatc gaagccgagg tccatcatct cccatgcggc ccaggcggtg gtgacgaatg
      661 acccaccgat cgagccgtcc atcttgctgc gggcacggtg ttcgatgcac agcagctcgg
      721 cgaggcgatc gtcctcgggt ttgggaatgc ccaggagccg ccgggcgatc acgccgggac
      781 gcgtgtccac accggaaaag agccgttcca ggcggacggt cgcctgttgc agggaagtcg
      841 tcatggagcc tccacttacg gaggtgtgat cgggaacttc cggtaaccgg gactgtgagc
      901 ggaggcgggc ggctcaaggg ctcgggcttg ccgcgcgatc tcaggtggct tgtagccccg
      961 tctcgaggaa aagttcccgt gcgaaatcgc tcccggcgct caccccaaac gccaggccag
     1021 gagcgcaccc gcgcccacat agcggactac cgcatggacg atggacacga acgacggccg
     1081 ggtcggcacc cgggtcgaca gaatgcccac caggccgaag agcagccacc atccgccgtg
     1141 ccccagactg tcgaaccaca gtgagccgaa cagtgtgacg agcagcgcct cgaccgccag
     1201 cgcgacgaac accgaagtcg tgacgggagc attggccacg acgcccccgc cgacgagccg
     1261 gttggcggtc caacccgcga ggcagaacca ggcggcgacc ggcagccagg cgagcaccaa
     1321 ccgcccgagg gtcatcgagc accgggcgga acgtatcccg gtccgcgcag gccgcgaccc
     1381 gggcgggcgg ctgtgagctc cccccgtttc aggacggtct gtccgttgac gagcacgtac
     1441 tcgatcccga ccgatggctg gtgcggcttg tcgaaggtcg cgcggtccgc cacggtcgcc
     1501 gggtcgaaca cggtgatgtc ggcgtacatg ccggggcgca gcaggccgcg atcgacgagg
     1561 ccgacccgct gcgctgcgag cgaggtcatc ttgcgcacgg cgaactcgag cgtcatgagc
     1621 cccgagtcgc gaacgtagcg gccgaggatg cgggggaagg tcccgtaggc gcgggggtgc
     1681 gccgattgcc cgccgagcgg gccgtccggc gccacgccgc cgaaatcggt gttgaccgcc
     1741 acccaccagg tcttcatcgc cgcctgcacg tcaggctcgc tcatgtcgaa gtagacggcg
     1801 tcggtgcggt gcgggccctc ggcgagcaga atgtcgaaga tcgtctcgat cgggtcgcgg
     1861 ttccgcgcgg ccgcgatctc gctgatgcgt tttccctgga gaccgcggag cgagtcttca
     1921 aagacgccgg tgatcaggat gccggctcca cctcccgcct cgttgtagaa cccttcgatg
     1981 cctcccgcgg ggcgcagcat ctccccgtgg atccgggcgc gggtggccgg gtcgcgcagg
     2041 tgcgccagca gcgagtcgag gccgccgctc tcggcccacg tcgggagact ggcgtcgagc
     2101 gacgtggccg cagcgatgta ggggtactgg tcggcggtga cgtcgagccc ggcccggcgg
     2161 gcggaatcga tccgcgccag gacggccggc atgcggcccc agctgcgccg tcccgcgacc
     2221 ttgagatggg agatctcggc ggggatgtcg gcctcgcgcg cgatccggaa cacctcgtcc
     2281 agggcctgat cgatccggct tccttcgttc cgcatatgcg aggcgtagat gccgccgtgc
     2341 cgccgcgcgg cccgcgccag ggcgatcagc tcctccgtcg tggaatagct cgcgggggcg
     2401 tattccagcg ccgaccagac gccgagcgcg ccctgcacca tggccgtgtc caccagcgcc
     2461 gccatgtgcg ccagctcggc agccgtcggc tggcgatctt cgctgccgat cacggcgagt
     2521 cgcacctgcg tggcgccgac gaacgtcgcg aggttgaccg tggagccgtt gcgggcgagc
     2581 tgggcgaagt agccgtcgaa gtcgcgccag tcctcccggt agcggtactt gcgcatggcg
     2641 tccgagtcgg gcaccaccag cgcatccgtg agcggtgcga ccgatccgcc ctcacccgtg
     2701 atttccgtcg tcacgccctg ggtgaccttg ctcagcaccc ggttgtcgat caggacgttg
     2761 atctcggatt ggcccatcat gtcgatgaac ccgggcgcga cgatcattcc ggacacgtcg
     2821 agggtctcgc gcgcggccac gtcgctgagt cggcccagcg ccgcgatgcg gtcgccgcga
     2881 atcgcgacgt cgccccgcca gcgaggattg ccggtgccgt ccaccaccca cccaccctgg
     2941 agcagcacgt cgtaggccgc gcctcccatg ggccgcgggc cgcgacccgc gggacgattg
     3001 caggcgaccc cgagcgccag ggcggccagg gccagggggg gcaggcgtct catttgctcc
     3061 aactggtcct cagggaatcg ggtcggaact ggcggccgcg caccatcacc caggcgatgt
     3121 cccgggtagc ggtgatgtcc tcactcggat tgcgattcag gaccacgagg tcggcgacct
     3181 tgcccgggga cagcatgccc agcgagtccg ccgccaggac ctgcgcgccg taccgggtag
     3241 cggaggtgat ggcgtccatc ggcgtcatgc cggctgccac cagcagcgcc atctcctcgt
     3301 gcagcgacgc gcccgggacg agcagcgggt tggcggcgtc ggtgccggcg gccacgagac
     3361 ccccggcggc gcgaaattcc cgcacgaact ggtcctgacg ggcgcgaccg cgacggaacg
     3421 cggcgatgtc ggcggcgcgc cacccggccc gttgcagcag ggcggaaacg gcccggaccg
     3481 gattgtcgcc ctccttcggc acgtcggcca tgccgaggcg ctgcaccaat gaggcgtcgt
     3541 cgagccgcgc gagtgtctcg tgctcgacca gcgtcgggac gatcgcgacg cgggtcgcgg
     3601 cgagcgcggc cgccagccgc gccacggcgc cggaatcgag cgccgcccag ctcgcctctt
     3661 ccatcgtcag gccggcgaag aagagatcgt tggcccgcag gaagggagcg ggatcgccgg
     3721 ccgcggcgga aacgacgccc gccagctgct ccagcgaggc cacgcccgag cgggccgcgg
     3781 tgagtgcgtc catcttgccg ggccgtgccg acacctggag gcggagcgtc gtggcctcgt
     3841 ccacgatcgc ccgcagcagc tcgggcgtga tcttggtgta ggccatgatg aaatcggcgc
     3901 ccgccacggc gcgttgatcc accgcctggc gcgcttcctc atcggcggcc accgcggtcc
     3961 cgcccggcag cgtggccgcc aaaccgtcga tcactggacc ggcggtgtac aggcgcggcc
     4021 cgaccaccga gcccaggtcg atgtccttct tgatggcgta accggaatcg cccaccgagt
     4081 ggacggcccg tacggacgtc acgccccagg cgaggtagcg gggcagggcc caccgctcca
     4141 cttcggcgtg tgcgtcgatg aggcccggca tgatcgtctt gccgaccaga tcgatgcgct
     4201 gcgccccgcg cggcaccttg atctcgttca cctgggccac ggcccggatg gtgccgtcct
     4261 tgatgaggat cagggcatcg gccttgggag gaccacccga gccgtcgatc agcgtcgccc
     4321 cctcgagggc gatcatgccc ttgccgggcg aggtgcaggc ggcggcgagc gccgtggccg
     4381 cgacgcggac cgccgcccgc caccacctca cggcttcggg tccgcggacg tgtgatcgtg
     4441 caggatctgc cagcggtcgc cgcgcttctg gaggatgagc gtgaacggcc cgctcgccac
     4501 cagcgagtcg ccccggtgca gctcgaagcg cgcggtcgcg taggccaggt ccgtggtcag
     4561 cgcccgcacc gcgatctcca cgaaggagag cgagtcccgg ctcttgccgg gtgcgaagta
     4621 ggtcgccttg tagtgatcgt acagcggctg ccagccgtac tggacgtggc cgccgctgac
     4681 gtagccgacc gtcgaatcct tcacatagtc gcccatgaag ccgtccggat caccgcgatt
     4741 ccaatccgcg gccgaccggg c
//
LOCUS       DBNGZW010000153         2381 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_832311, whole genome shotgun sequence.
ACCESSION   DBNGZW010000153 DBNGZW010000000
VERSION     DBNGZW010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2381)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2381)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2381
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_832311"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..872)
                     /locus_tag="AC16JC_03740"
                     /pseudo
     CDS             complement(79..872)
                     /locus_tag="AC16JC_03740"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(951..1115)
                     /locus_tag="AC16JC_03745"
     CDS             complement(951..1115)
                     /locus_tag="AC16JC_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163841.1"
                     /translation="MRSQTVFARGAVEQHRADRRAAGARIRANRETTLLGTVINRARV
                     WELYSGPNPV"
     gene            complement(1184..1516)
                     /locus_tag="AC16JC_03750"
     CDS             complement(1184..1516)
                     /locus_tag="AC16JC_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163842.1"
                     /translation="MIAATYARLLFVGLGLLTCATSASAECAWVQWTMVNGPRGVWQP
                     LSAFGTREQCIAHMAVASTDFAGKTVNTVAGSIDTGEAAYVDKRGKITFLEKCLPDTV
                     DPRGAKGK"
     gene            complement(1847..2044)
                     /locus_tag="AC16JC_03755"
     CDS             complement(1847..2044)
                     /locus_tag="AC16JC_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163843.1"
                     /translation="MSAPVDREQLRRELPDAQSAIQRAHDVTGLDALAASLQTLAEAP
                     YAFHGRLLQKGLAEMPRTIRR"
     gene            complement(2041..2226)
                     /locus_tag="AC16JC_03760"
     CDS             complement(2041..2226)
                     /locus_tag="AC16JC_03760"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5163844.1"
                     /translation="MALKLRRLRTKQQMSQAELAAKARISRGYLIRLENARQDPTLGT
                     LGRLAKALGVPITELLG"
BASE COUNT          360 a          839 c          774 g          408 t
ORIGIN      
        1 catcgctgcg gggtctcctt tcagtctgag gctctcccgg tggtcggcga ctacggtagg
       61 tggtgggtga acgacctgtc aatcgcttcc gcggccgcac gactggagat ttcgcgtgga
      121 ctcgccgggc gcaaatggcc cgggttgtcc tgttgggagc cggggcgcga ggagcatccg
      181 ggtgaggcta cgcctggtga ggcgcctaga attcggcgcc gttactgacg acgatgaagt
      241 ggtgcccccg gggtgactcg aacacccggc acgcagttta ggaacccacg gtgccggacg
      301 cgcttcgcct aacccttccg tcgcgttagc gcgaccaccc ttcggggcga tagggacttc
      361 cggagggacg ggaaacgctg cggccatccg ctgcacagcc gcctgcacat gcgcgtccgc
      421 gaggtgggag taccgcgtca ccagatcgag cgtggcccag ccgcccaact cctggagcgt
      481 ccgcagatcc gccccgctca tcgccatccg gctggcccag gtatgccgca acgtgtgcgg
      541 cgacacgccc tcgagcccag cccgctggca ggcgcgccga aagacactgc gaagcgacgg
      601 agcggctgcc cggtgcgcgt cgtgaacacg tgcccatgaa tcggggtcgc cccgtcgcgg
      661 gcccgggcct gggcggccgc cgcaaatccc tcgcgcaggc gcggcgtcag cggcacgtgg
      721 cgctccttgc tgctcttggc ggcctcggcc ttgagcgtga gccggccgcc ctcgcggtcc
      781 acgtcctgcc aggtgagcgc gagggcctcc gagaccgcgc gtggcccgca gtcgatgcag
      841 accatgacca gcaaccgata gtgcgcgggc acggcggcca ggagccgggc ctcctcctcg
      901 accgtcaaga agcgcagccg ccgctgtggt tcctggagcc gtcggatccc ctacaccggg
      961 ttcggcccgc tgtagagctc ccagacgcgg gcgcggttga tgacggtgcc gagcagggtc
     1021 gtttcccggt tcgcccggat gcgggcgcca gcggcccggc gatcggcgcg gtgctgctcc
     1081 accgccccgc gagcgaagac cgtctgtgag cgcattggaa ggcccctgtg tccagccgtc
     1141 cgagcgtgaa ctgcacgaat tgggcgattt cgacggtcgc ccctcacttt cccttcgccc
     1201 cacgtgggtc cacggtgtcg gggaggcact tctccaagaa cgtgatcttg cctcttttat
     1261 cgacatacgc ggcctcgccc gtgtcgatgc ttccggccac ggtattgacg gtcttgccag
     1321 caaagtcagt gctggctacc gccatgtgag cgatgcattg ttcacgcgtg ccgaatgccg
     1381 acagtggttg ccacactccc cgtggcccgt tgaccatcgt ccattgcacc cacgcgcatt
     1441 cggcggaggc cgaggtggcg caggtgagca ggccgagccc gacgaacagg aggcgcgcgt
     1501 aggtggcggc gatcatttgc tcctccgtga gtccgcggcg ccccgcctga gatgtcgtgc
     1561 gccccacaac gccgccgctg ctatcgcgag gctgacgccg tccgagatcg cgctctggac
     1621 gagactgaag cccggcgtgt gatatccgat tttcgctgcc cacagggtca cgacgaggcc
     1681 aagcaggcca taaaccatcc cgaggattcc tgcgcccgtc aacagccagt ctgccagcac
     1741 cacataccac gctcgacctt tcatccgttt tcggttcgtc acagcccgac gtcttcttac
     1801 gtcacaccgc gcccgagctt ggcgatcacc tgcaccaggc tccagatcag cgccgaatag
     1861 ttcggggcat ctccgccagg cccttctgca ggaggcggcc gtggaaggcg tacggggcct
     1921 cggctagcgt ctggagcgac gcggccaacg catccagccc ggtcacgtcg tgggcgcgct
     1981 gaatggcgct ctgggcgtcg gggagctccc gcctcagctg ctcgcgatcg accggggcgc
     2041 tcacccgagc agctccgtga tcggcacgcc gagggccttc gccaagcgcc ccagggtgcc
     2101 gagggtcgga tcctgccggg cgttttccaa acgtatgagg tagccccggc taatccttgc
     2161 cttcgcggcg agttcggcct ggctcatctg ttgcttcgtt cggagccgcc gcagtttcag
     2221 cgccatccgc tttaccggag gcatagtcac ctataagtga cctactgtca agctggccgt
     2281 gggacgcaca ggcttatcca ccgtcaccgc cgcccctcct gcgcctcagc tcgtgccatc
     2341 tcctcctcgg tcacacacag cgcgcccgga gccgctacct t
//
LOCUS       DBNGZW010000154        10331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_151506, whole genome shotgun sequence.
ACCESSION   DBNGZW010000154 DBNGZW010000000
VERSION     DBNGZW010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 10331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10331
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_151506"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2099)
                     /locus_tag="AC16JC_03765"
     CDS             complement(<1..2099)
                     /locus_tag="AC16JC_03765"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5163845.1"
                     /translation="MRYVRAALRRLAGIFAGHRADDELREELQAHLDMETAENIRRGA
                     TPDEARRQALLASGGLAQAAEAVRDRRGLPWIEHLAADVRYALRTLRHSPAFTIVVVI
                     TLALGIGANTAIFSVVRGVLLKPLPNRNGDRLLYLRQSSDGPGQANISFSVPEVGDLR
                     TGVPSLGGIAEYSPWALTLQGDDDAVRIDVGLVTGNYFDVMGLAPVLGRLTRPSDDGP
                     GVPPVMILTHAFWIKRFGGDSSVVGRQVKLEGQSVTVIGVLQPAPFFPDRIDALLNMV
                     VSPHHVSAMMIQNRSHRMTEVVARLAPGASVAQARTEVAAVYARMQHDHTDAYNPGSH
                     YRLAVIPFKEALGERARLTLWLLMGAAAFVMIISAANVANLTLMRGVRREHELVVRAA
                     LGAGVARLRRLLLVENLLLTLVGAVLGVVIALGGVRLLIALAARYSPRAGEIHLDGVV
                     LGFTLTLSVTLALLLSFIAALPQEGTFASWISAGVRRISGSLKKHRLQRGLVVAQVAV
                     SVVLLAGAGLLTRTMMRLSAVDTGLRAEEVLALEVPLLKLGGAPSDASDPAAWMRHLL
                     EADAAAKQEYERMRVAIRALPGVSEAGLGSSMPLATSMMSFEVKAEARALAVGEAAPH
                     AAVRTASPEYFRASGIPLLGGREFTSTDRAGSGLVVIVNKTFADKFFPGEDPIGKRIA
                     WTGELLKFSPISGDWRT"
     gene            complement(2096..2440)
                     /locus_tag="AC16JC_03770"
     CDS             complement(2096..2440)
                     /locus_tag="AC16JC_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR5163846.1"
                     /translation="MADKLDVQPGTLALMILKTLEVLGPLHGYGVARRIEETAKHRLT
                     LNYGTLYPALLKLEQEGFITAEWRQSENNRRAKFYALTAAGRRQLARETREWHQIAEL
                     IAAFLAPQREAP"
     gene            complement(2499..2885)
                     /locus_tag="AC16JC_03775"
     CDS             complement(2499..2885)
                     /locus_tag="AC16JC_03775"
                     /inference="COORDINATES: protein motif:HMM:NF018672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HIR5163847.1"
                     /translation="MILLMWLAALAAGLIHLLIFCMESLWWTTPQVRARFRQTPEQAA
                     TTRLLAFNQGFYNLFLGLGIFAGLALVPLGHPQAGLILVSWNCLFMIGAAIVLARSAP
                     RMWRGALIQGAAPLLYLALAAIQRAG"
     gene            complement(2882..3511)
                     /locus_tag="AC16JC_03780"
     CDS             complement(2882..3511)
                     /locus_tag="AC16JC_03780"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008316512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HIR5163848.1"
                     /translation="MTLGPSPETRFPLPGVTRTVFLRPFITRPNIIVGDYTYYDDPDG
                     PEQFERHVLYHFDFIGDRLIIGRFCCIAAGVRFIMNGGNHATTWLTTYPFPIFSQGWD
                     LPEPPTWPNKGDTTVGNDVWLGNSATVMPGITIGDGAVVATSAVVTKDVPPYAIVGGN
                     PATVLRYRFDPPTIARLLALRWWDWEPAKIARNVALLCGGDIEALERSE"
     gene            complement(3508..3954)
                     /locus_tag="AC16JC_03785"
     CDS             complement(3508..3954)
                     /locus_tag="AC16JC_03785"
                     /inference="COORDINATES: protein motif:HMM:NF019932.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR5163849.1"
                     /translation="MANSIDARTSADQILMLELGALDRWGKGDPGGFLELYAADITYF
                     DPLTATRIDGHQAMANYYLPWVGKINIVRYEMLNPQIVVEGNMALLTYNLVNYTRDAE
                     GLEAVGSCWNSTTVYQHRSGRWKAIHSHWSFTRHTAFQNMSPEASP"
     gene            4243..4542
                     /locus_tag="AC16JC_03790"
     CDS             4243..4542
                     /locus_tag="AC16JC_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010583643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR5163850.1"
                     /translation="MPTYEYKCPKGHLFEKFYPTITSKRTATCPTCGKKAERQLSGGG
                     GLVFKGSGFYITDYKRAGEKKESESKPADKPAEKQPEAKPESKPESKSKKDSPKK"
     gene            4539..6182
                     /gene="argS"
                     /locus_tag="AC16JC_03795"
     CDS             4539..6182
                     /gene="argS"
                     /locus_tag="AC16JC_03795"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019139835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HIR5163851.1"
                     /translation="MNAQDSLRAALVRAAASLGVPTTDVLLERPKDPSHGDVATNLAL
                     TLAKQLKQKPRDVAAKLVAALELPPGLVRKTEIAGPGFINFFLAAEQLHAILPTVIAA
                     GKDFGKSDVGHGAPVNVEFVSANPTGPLHVGHGRQAAIGDAIAALLEWTGWKVTREFY
                     YNDAGTQIANLALSVQARIAELRGRPAEIPEGGYHGAYIRDIAQAYVAAHPNDEDGAD
                     LEAIRRFAVDELRQEQDKDLQSFGVKFDVYFLESSLYADGAVDKTVKQLQSAGHTFEE
                     EGALWLRTSDFGDDKDRVMKKSADRGGDYTYFVPDVAYHVNKWRRGFTRAINVQGADH
                     HSTVTRVRVGLQALEMGITPGYPEYVLHQMVTVMKGGEEMKISKRAGSYVTVRDLVDE
                     VGADALRFFYLMRRSESHLVFDVDLAKSQTEENPVYYVQMAHARMSGIFRVAARAPET
                     ATPDGADLGALAEPDELDLMKTLAEWPAMVQGAAAALEPHRITGYLETLTRLAHGWYQ
                     KHRVLGQEEEAARLVLARAVRQVLANGLTLLGISAPDRM"
     gene            6186..7094
                     /locus_tag="AC16JC_03800"
     CDS             6186..7094
                     /locus_tag="AC16JC_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020675757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HIR5163852.1"
                     /translation="MTLLVVGSIALDSVATPFGQTADAPGGSAVFFAAAGCLLHPVQV
                     VGVIGSDYPLGVLKQLETRGVDLTGVEQVDGESFRWKAKYSYDLSSRETLDTRLGVFA
                     HFRPKIPAAFQQAKYVFLGNIDPELQLGVLDQVADPELVACDTMNYWINSKKERLLEL
                     LRHIDILMVNDTEARELSGDWNIYRAAHWILERGPKRVVIKQGEHGALLVDPETTFKV
                     PAYPLQEVFDPTGAGDAFAGGFMAYLASVKLLSTPALRRAMVHGAAMGSFSVEAFGIQ
                     GFDTVTLADVRARVRAFKELVHFELD"
     gene            7081..8112
                     /gene="purM"
                     /locus_tag="AC16JC_03805"
     CDS             7081..8112
                     /gene="purM"
                     /locus_tag="AC16JC_03805"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013163308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HIR5163853.1"
                     /translation="MSSTDRYASAGVHLDAAEQAKRRIQALVAQTRTPLSLGALGGFG
                     GMVRVPEGFTSPVLVMSTDGVGTKVLVAALAGVHDTVGEDLVNHCVNDILVHGAQPLA
                     FMDYLASGQLDPDVAEAVVQGISRGCVAHEMTLAGGETAELPDLYAPGHYDLAGTIVG
                     IVEEGAALHGDKVQPGDALVGYASSGLHTNGYTLARHIVFDQLHLGVHDRFPDTEGTV
                     AEVLLAVHCSYWKAVRPMLPRVHALAHITGGGIAGNLVRVLPAGCEAVVAAGSWAWPP
                     VFRVLKTAGDVSIPEMRRVFNLGIGMIAVVGRDDVDDTRRYAERAGVRTWIIGEVVPG
                     ERGVRFSER"
     gene            8275..9225
                     /locus_tag="AC16JC_03810"
     CDS             8275..9225
                     /locus_tag="AC16JC_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163854.1"
                     /translation="MQRKFVTLAVLSSLATIAPLHAQCTGSPAVVAVCNRAVDALKTF
                     HPAVGLVIAGGNPELGGGRAFNGFGHFFVSARVNAAKVTAPNPDTSAGPTSLKGVVPA
                     PIVEAGVGLYRGLPSGLLAVDGLFSATLVPTHVVDKLDVDSGATKLGSMALGLGYGVR
                     VGIVRGLFPIPSMSISVMRRSLPRVTYGRLAPTALSAGDAFEFDSDVHATTWRFVAGY
                     HLVAADVAVGLGFDRYTSHGRLRYYDNPPFNSVATVPFDPVNERQLIFADAAFHLAVF
                     TVGAELGWQTGEDQKLSTIYTDFDPSAGHLFGGVGLRFGF"
     gene            9248..10321
                     /gene="ribD"
                     /locus_tag="AC16JC_03815"
     CDS             9248..10321
                     /gene="ribD"
                     /locus_tag="AC16JC_03815"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR5163855.1"
                     /translation="MQRALELARRGWGRVFPNPLVGAVVLRDRMVVGDGWHAEFGGDH
                     AEVMALRAAGTRAGGSTLVVTLEPCAHHGKTPPCTDAILATGVRRVVAAVRDPDVAAR
                     GGLALLREAGLETATGVLTDAAVALNAPFFFVRENQTRPFVALKLATSIDGRIADVGG
                     RSQWISGPQARDWAHWLRAGFDAIAVGGTTAGRDDPQLTVRGGIVPRRAPVRIVFDRR
                     ANLSLTSKLARTAGDPPTWVVASEEAPVENVQGLEASGVRVFRPRSLGEGLSELRAAG
                     VESLLCEGGGALGVGLLADQLVDRLYWLQAPVWLGAGGVPAFPGVADAALPEAQRWIP
                     VERRPLGSDTLLVLDRRLCLPGS"
BASE COUNT         1664 a         3428 c         3620 g         1619 t
ORIGIN      
        1 atcgtacgcc agtcgccgct gatggggctg aacttgagca gctcaccggt ccaggcgatc
       61 cgcttcccga tcgggtcctc gccggggaag aacttgtcgg cgaaggtctt gttgacgatc
      121 accaccaacc ccgagccggc ccggtcggtg ctggtgaact cacgcccgcc gagcagcgga
      181 atccccgagg cgcggaaata ctcggggctc gccgtgcgga cggcggcgtg cggcgcagcc
      241 tcccccacgg cgagggctcg ggcctccgct ttgacctcga acgacatcat cgaggtggcg
      301 agcggcatgc tcgagcccag gcccgcttcg gagacgcccg ggagggcccg gatcgcgacc
      361 cgcatgcgct cgtactcttg cttggccgcg gcgtcggctt ccaggagatg tcgcatccat
      421 gcggcagggt ccgaggcatc gctcggcgcc cctcccagtt tgagcagcgg cacctccagg
      481 gcaagcactt cctcggcccg gaggccggtg tccaccgcgg acaaccgcat catggtgcgc
      541 gtgagcagtc cggcgcccgc gagcagcacg acggacacgg cgacctgggc cacgacgagg
      601 ccgcgctgca gccggtgctt cttgaggctc ccgctgatgc gccgcacccc ggcggagatc
      661 cacgaggcga aggtcccttc ctgcggcagc gccgcgatga acgagagcag cagggcgagc
      721 gtgaccgaaa gcgtgagggt gaagcccagg acgacgccgt cgagatgaat ctcgcccgcg
      781 cggggcgagt accgcgcggc cagcgcgatg agcagtcgca ccccgccgag ggcgatgacc
      841 acgccgagga cggcccccac gagcgtcagc aggagattct cgaccagcag caagcgccgc
      901 aggcgcgcca cgccggcgcc cagcgccgcg cgcaccacca gctcgtgctc ccggcgcacg
      961 ccgcgcatca gcgtcaggtt ggcgacgttg gcggcggaga tgatcatgac gaacgcggcg
     1021 gcacccatga gcagccagag cgtcagccgt gcgcgctcgc ccagggcttc cttgaaggga
     1081 atgaccgcga ggcggtaatg agagccggga ttgtacgcgt ccgtgtgatc gtgctgcatg
     1141 cgggcgtaga cggcggcgac ctcggtgcgg gcctgcgcca cgctggcgcc cggtgcgagc
     1201 cgggccacca cctcggtcat gcgatgcgag cggttctgga tcatcatcgc gctcacgtga
     1261 tgcgggctca ccaccatgtt gagcagcgca tcgatccggt cgggaaagaa cggcgcgggc
     1321 tggagcacgc cgatcaccgt caccgactgg ccctccagct tcacctgcct gccgacgaca
     1381 ctcgagtctc cgccaaagcg tttgatccag aaggcgtgcg tgaggatcat gacggggggc
     1441 acgccggggc cgtcgtcgct gggccgggtg agtctgccga gtaccggcgc gagtcccatg
     1501 acgtcgaagt agttcccggt cacgagtccc acgtcgatgc ggaccgcgtc gtcgtcaccc
     1561 tggagggtga gcgcccacgg ggagtactcc gcgatgccgc ccagggacgg cacgccggtc
     1621 cggagatcac cgacctccgg aacggagaag gagatgttcg cttgcccggg tccgtcggag
     1681 gactgccgca ggtagagcag ccgatcgccg ttgcggttgg gaagcggctt gagcagcacg
     1741 ccgcgaacga cgctgaagat cgcggtgttg gcgccgatcc cgagcgccag ggtgatcacc
     1801 accacgatgg tgaaggcggg actgtggcgc agcgtccgca gggcgtagcg gacgtcggcg
     1861 gccaggtgct cgatccaggg cagaccgcgc cgatcgcgca ccgcctctgc ggcctgggcg
     1921 aggccgccgg acgccagcag cgcctggcgg cgtgcctcgt cgggcgtagc ccctcgccgg
     1981 atgttctcgg cagtctccat gtcgagatgg gcctgcagct cttcgcgaag ctcgtcgtcg
     2041 gcgcggtgtc cggcgaagat gccggccagg cgccgcagcg cggcgcggac gtatctcatg
     2101 gggcctcccg ttgcggcgcg agaaaggcgg cgatcagctc ggcgatctgg tgccattcgc
     2161 gggtctcccg cgccagctgc ctgcgccccg cggcggtgag cgcgtagaac ttcgcgcgac
     2221 gattgttctc ggattggcgc cactccgccg tgatgaaccc ctcctgctcg agcttcagga
     2281 gggcggggta gagggtgccg tagttgaggg tgaggcggtg cttggcggtt tcctcgatgc
     2341 ggcgcgcgac gccgtagccg tgcagcggtc ccaggacctc gagggtcttg aggatcatca
     2401 gggcgagggt gccgggctgt acgtccagtt tgtccgccat ggtccgtcgt atgggtttcc
     2461 catacgacgg caagcggcgg gattagtttc acgacccctt agccggcccg ctgtatcgcg
     2521 gcgagcgcca gatagagcag gggagccgcg ccctgaatga gcgccccgcg ccacatgcgg
     2581 ggcgccgagc gcgccagaac gatcgcggcg ccgatcatga agaggcagtt ccagctgacg
     2641 aggatgagcc cggcctgcgg atgtcccagc gggaccaggg cgagcccagc gaagatgccg
     2701 aggccgagaa agagattgta gaacccctgg ttgaacgcga gcaacctggt cgttgcggcc
     2761 tgctccggcg tctgacggaa gcgcgcgcgg acctgcggcg tggtccacca caggctctcc
     2821 atgcagaaga tgagcaggtg aatcaggccg gcggctaacg ccgccagcca catcaggaga
     2881 atcattccga gcgctccagg gcctcgatgt cacccccgca gagcagggcg acgttccggg
     2941 cgatcttcgc gggttcccaa tcccaccagc gcagggcgag gaggcgggcg atcgtgggtg
     3001 ggtcgaaccg gtagcgcaga acggtggcgg gattgccgcc gacaatcgca tatggcggga
     3061 cgtctttggt cacgaccgcg ctggtcgcga ccacagcacc gtccccgatg gtgatgccgg
     3121 gcatgaccgt tgcggaattc ccgagccaga cgtcgttgcc gaccgtggtg tctcctttgt
     3181 tgggccacgt aggcggctcg ggcaggtccc agccctgtga gaagatcggg aacgggtacg
     3241 tcgtcaacca tgtagtggcg tggttgccgc cgttcatgat gaaccggaca cccgcagcga
     3301 tgcagcagaa gcgcccgatg atgagtcgat caccgatgaa atcgaagtga tacaggacgt
     3361 ggcgctcgaa ctgctccggg ccgtctggat catcgtagta ggtgtagtca ccgacgatga
     3421 tgttcggtcg agtgatgaac ggccggagaa agacggtgcg ggtgacgcca ggcagcggaa
     3481 accgggtttc aggggatggt ccgagcgtca tggagacgcc tcaggcgaca tgttctgaaa
     3541 cgcagtgtgg cgagtgaacg accagtgcga gtggatcgcc ttccatctac cactacggtg
     3601 ctgatatacc gtcgttgaat tccagcaact ccctactgcc tcaagacctt cggcatcccg
     3661 tgtgtaattc accaggttgt atgtcaggag cgccatgttg ccttcgacaa ctatctgcgg
     3721 gttgagcatc tcgtagcgga cgatgttgat cttgccgacc catggaaggt agtaattcgc
     3781 catcgcttga tgcccgtcaa tgcgcgtcgc cgtcagcggg tcgaagtagg tgatgtcggc
     3841 cgcatatagt tccaagaatc cgccgggatc gcctttgccc caccggtcga gagcgccgag
     3901 ctcgagcatg aggatctgat cggcactcgt tcgggcatcg attgaattgg ccacgcgatc
     3961 ctccgttgta tgcggcctga ctcggaattg gactgccaag ttcgaattag cgataagcct
     4021 cgccagcagt aacctgcact gcaatcgtct ccggctgagt aatgtccacc aagcctctcg
     4081 gtctcggaaa tgctgcagcg tcagcgagcg gcgtaagccc ggcaccgcgc agctcaacgc
     4141 gcggcccgag aaccctgcgc tttcaagctc taaccggcag cggcgccgcg gaacgcgaac
     4201 ctcaattgac cacctgcctc gtggtcgcta agttctttcc caatgccaac atacgagtac
     4261 aagtgcccca aggggcacct gttcgagaag ttctacccga cgatcaccag caagcggacc
     4321 gctacctgcc cgacctgcgg caagaaggcg gaacgccagt tgtcgggcgg cggcggattg
     4381 gtcttcaaag gctccggctt ctacatcacc gactacaagc gggccggtga aaagaaggaa
     4441 agcgagtcga agccggccga taagcccgcg gagaagcagc ccgaagccaa gccggaatcg
     4501 aagcccgagt cgaagtcgaa gaaggactct cccaagaagt gaacgcccag gactccctgc
     4561 gcgcggcgct cgtccgggcc gcggcgtccc tcggcgtgcc cacgaccgac gtcctgctcg
     4621 agcggcccaa ggacccgagc cacggcgacg tggccaccaa cctcgccctc accctcgcga
     4681 aacagctcaa gcagaagccg cgcgacgtgg ccgcgaaact ggtcgcggcg ctcgagctgc
     4741 ctcccggcct cgtgcgcaag acggagatcg cgggccccgg attcatcaac ttctttctcg
     4801 cggctgagca gctgcacgcg attctgccca ccgtgatcgc ggcggggaag gacttcggca
     4861 agagcgacgt ggggcacggc gccccggtga acgtggagtt cgtctcggcg aatccgacgg
     4921 gcccgctgca cgtcggccat ggccgccagg ccgcgatcgg cgacgcgatc gccgcgctgc
     4981 tcgaatggac cgggtggaag gtcacccgcg agttctacta caacgacgcc ggcacccaga
     5041 tcgcgaatct ggcactgtcg gtccaggcgc gcatcgcgga gctgcgcggc cgccctgccg
     5101 agatccccga gggtggttac cacggcgcat acatcaggga catcgcccag gcgtacgtcg
     5161 cggcgcaccc caacgacgag gacggcgcgg acctcgaagc gatccgccgc ttcgccgtgg
     5221 acgagctgcg ccaggagcag gacaaggacc tgcagtcgtt cggcgtgaag ttcgacgtct
     5281 atttcctcga atcgtcgctc tacgccgacg gagcggtcga caagaccgtg aaacagctcc
     5341 agtccgcggg gcacaccttc gaggaagagg gcgccctctg gctccgcacg tcggacttcg
     5401 gcgacgacaa ggaccgcgtg atgaagaagt cggcggacag gggcggagac tacacgtact
     5461 tcgtccccga cgtcgcttac catgtgaaca agtggcgccg cggcttcacc cgcgccatca
     5521 acgtgcaggg cgctgatcat cattcgaccg tgacgcgggt gcgcgtgggg ctccaggcgc
     5581 tggagatggg gatcacgccg ggctaccccg agtacgtgct gcaccagatg gtgaccgtga
     5641 tgaaaggcgg cgaggagatg aagatctcca agcgcgccgg cagttacgtc accgtgcggg
     5701 acctggtaga cgaggtcggg gccgatgcgc tgcgcttctt ctatttgatg cgtcgctcgg
     5761 aatctcacct cgtcttcgac gtggacctcg cgaagtccca gaccgaggag aatcccgtct
     5821 actatgtgca gatggcgcac gcgcggatga gcggcatctt tcgcgtcgcg gcccgggcgc
     5881 cggaaaccgc gacgccggac ggcgccgacc tgggcgcgct cgcggagccc gacgagctgg
     5941 atctcatgaa gacgctcgcc gaatggccgg cgatggttca gggcgcggcg gccgccctgg
     6001 agccgcaccg tatcaccggc tacctcgaga ccctgacgcg attggcccat ggctggtacc
     6061 agaagcaccg ggtgctggga caagaggaag aagccgcccg tctggtgctg gcccgcgcgg
     6121 tgcggcaggt gctcgccaac ggactcacgc tcttggggat cagcgccccg gaccggatgt
     6181 agcccatgac cctcctcgtc gtcggctcga tcgcgctcga ctcggtcgcc acccccttcg
     6241 gccagaccgc cgacgcgccg ggcggctccg ccgtcttctt cgccgccgcc ggctgcctgc
     6301 tgcacccggt gcaggtggtg ggcgtcatcg gctccgacta cccgctcggc gtgctcaagc
     6361 agctggagac gcgcggcgtg gatctcaccg gcgtggagca ggtcgacggc gaatcgttcc
     6421 gctggaaggc gaaatactcc tacgacctct ccagccgcga gacgctcgac acccgcctcg
     6481 gcgtgttcgc ccacttccgg cccaagatcc cggccgcgtt ccagcaggcg aagtacgtct
     6541 tcctcggcaa tatcgatccc gagctgcagc tgggcgtgct cgaccaggtg gccgatcccg
     6601 agctcgtggc gtgcgatacg atgaactact ggatcaactc gaagaaggag cggctgctgg
     6661 agctgctgcg ccacatcgac attctgatgg tgaacgacac cgaggcgcgc gagctctcgg
     6721 gtgactggaa catctaccgc gccgcccact ggatcctcga gcgcgggccc aagcgcgtgg
     6781 tgatcaagca gggggagcac ggcgcgctgc tggtggaccc cgagacgacg ttcaaggtgc
     6841 cggcgtaccc gttgcaggaa gtgttcgatc ccaccggtgc gggcgacgcg ttcgcgggcg
     6901 ggttcatggc ctacctggcg agcgtgaaac tcctctccac tccggcgcta cgccgcgcca
     6961 tggtccacgg cgccgcgatg ggcagctttt cggtggaagc cttcggcatc caggggttcg
     7021 atacggtcac gctggccgac gtccgtgccc gggtgcgcgc gttcaaggag ctggtgcact
     7081 ttgagctcga ctgaccgcta cgcttcggcc ggcgtccacc tcgatgccgc cgaacaggcc
     7141 aagcgccgca tccaggcgct ggtggcgcag acgcgcacgc cgctctccct cggggcgctg
     7201 ggcgggttcg gtggcatggt gcgcgtcccg gagggattca cgtccccggt gctcgtgatg
     7261 agcaccgacg gcgtgggcac caaggtcctg gtggccgcgc tcgccggcgt ccacgacacc
     7321 gtgggcgagg atctcgtgaa ccactgcgtg aacgacattc tggtccacgg cgcgcagccg
     7381 ctcgccttca tggactacct cgccagtggg cagctggacc cggacgtggc cgaagcggtg
     7441 gtgcagggga tcagccgcgg gtgcgtggcg cacgagatga ccctggccgg cggcgaaacc
     7501 gccgagctcc ccgatctcta cgccccggga cattacgatc tggccggcac gatcgtcggc
     7561 atcgtcgagg aaggggccgc gctgcacggc gacaaggtgc agccgggtga tgcgctcgtc
     7621 gggtatgctt cgagcggcct gcacaccaac ggctacacgc tggcgcgcca tatcgtattc
     7681 gaccagctgc acctgggcgt gcacgatcgg tttccggaca ccgaagggac cgtggccgag
     7741 gtgctgctcg ccgtgcactg cagctactgg aaggcggtgc ggcccatgct gccgcgtgtc
     7801 catgcccttg cccacatcac gggcggtggg atcgcgggca acctggtgcg cgtgctgccc
     7861 gccgggtgcg aggcggtggt ggccgccggg tcatgggcgt ggccgccggt gtttcgcgtg
     7921 ctcaagacgg cgggagacgt ctcgattccc gagatgcgcc gcgtcttcaa cctcggtatc
     7981 gggatgatcg ccgtggtagg gcgcgacgac gtcgacgaca cccgccgcta cgccgagcgc
     8041 gccggcgtgc gcacctggat catcggggag gtggtaccgg gagagcgcgg ggtgagattc
     8101 tcggagagat gaaacgggat ccgggacccg ggatccctga tccgtgtacc ggaccccgga
     8161 tcccggatcc cggatcccgg atcccggatc ccggaccccg gaccccggat cccggatccc
     8221 ggaccccggg cccctgaccc cgggcccctg accccggact cactggaggt gggagtgcaa
     8281 cgaaagttcg tcacactggc cgtcctgtcg tctctcgcca caattgcgcc gctccacgcc
     8341 cagtgcactg gaagcccggc ggtcgtggcg gtgtgcaacc gcgcggtcga cgccctgaag
     8401 acgtttcatc ccgcggtggg gctcgtcatc gccggcggca atccggagct ggggggaggc
     8461 cgcgccttca atggcttcgg gcacttcttc gtctcggccc gggtgaacgc ggcgaaggtc
     8521 acggccccga accccgacac ctcggcaggc ccgacctcgc tcaagggcgt cgtgccggcg
     8581 ccgatcgtcg aagccggtgt ggggctgtat cgcggtctcc ccagcggcct cctcgccgtg
     8641 gacgggctgt tctcggccac gctggtgccg acccatgtgg tggacaagct cgacgtggat
     8701 tcgggggcca cgaagctcgg cagcatggcg ctggggctgg gctacggcgt ccgggtggga
     8761 atcgtgcgcg ggctgttccc gatcccgtcc atgtccatca gcgtgatgcg ccgctcgctg
     8821 ccgcgggtca cctacggccg gctggcgccg acggcgctct ctgcgggcga cgccttcgaa
     8881 ttcgacagcg acgtccacgc caccacgtgg cgcttcgtgg cgggctatca cctcgtcgcg
     8941 gcggacgtgg cggtcggcct cggcttcgac cgctacacgt cgcacgggcg gctgcggtac
     9001 tacgacaacc cgccgttcaa cagcgttgcc accgtgccgt tcgatcccgt gaacgagcgg
     9061 cagctgatct tcgccgacgc cgccttccac ctcgccgtct tcacggtcgg cgccgagctc
     9121 ggctggcaga cgggtgagga tcagaagctg agcacgatct acaccgactt cgatccctcc
     9181 gcgggacatc tttttggcgg cgtcggcctc cgcttcgggt tctgacgaag cgctcgagcg
     9241 ctcggcgatg cagcgggcgc tcgagctggc ccgccgcggg tggggtcgcg tgttccccaa
     9301 tccgctggtg ggtgccgtcg tgttgcgcga ccgcatggtg gtgggcgacg gctggcacgc
     9361 cgaattcggc ggagaccacg ccgaggtgat ggcgttgcgt gccgccggaa cgcgggccgg
     9421 cggatccacg ctcgtggtga cgctcgagcc gtgcgcccat cacgggaaga cgcccccgtg
     9481 caccgacgcg atcctcgcga cgggagtgcg ccgggtcgtg gccgcggtgc gcgatcccga
     9541 cgtcgcggcg cggggcggcc tcgcgctgct gcgtgaagcg ggactcgaga cggcgacggg
     9601 agtactgacc gacgcggccg tggctctcaa cgcgccgttc ttcttcgtgc gcgagaacca
     9661 gacgcggccg ttcgtggcgc tgaagctcgc gacctcgatc gacgggcgca tcgccgacgt
     9721 cggcggccgc tcgcaatgga tctcggggcc gcaggcgcgc gactgggcgc actggctgcg
     9781 cgccgggttc gacgcgatcg cggtgggcgg caccaccgcc ggccgcgacg acccgcagct
     9841 caccgtgcgc ggcgggatcg tgccgcggcg cgcgccggtg cggatcgtgt tcgaccgccg
     9901 cgccaacctg agcctcacct cgaagctcgc gcggaccgcg ggcgatccgc cgacctgggt
     9961 cgtggcgtcg gaggaggcgc ccgtcgagaa cgtccagggc ctggaagcgt cgggcgtgcg
    10021 cgtgttccgg ccgcgctccc tcggcgaagg gctctccgag ctgcgcgccg cgggcgtcga
    10081 gtccctgttg tgcgaagggg gcggggcgct gggcgtcgga ctcctggccg accagctggt
    10141 ggatcggctc tactggctgc aggcgccggt gtggctcggg gcgggcgggg tgccggcgtt
    10201 tccgggcgtg gccgatgcgg cgctgcccga ggcgcagcgc tggattcccg tggagcgccg
    10261 tcccctgggc tccgatacat tactggtatt ggaccggcgg ctatgtttac cgggatcgta
    10321 ggtgcggtgg g
//
LOCUS       DBNGZW010000155         2583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1583967, whole genome shotgun sequence.
ACCESSION   DBNGZW010000155 DBNGZW010000000
VERSION     DBNGZW010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1583967"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="AC16JC_03820"
     CDS             complement(<1..472)
                     /locus_tag="AC16JC_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5163856.1"
                     /translation="MAGAERLLEPLLSLHDRVRAAVVDACARQAADELAAVAADETAG
                     GGDTIYAVDRVAEEVFLQGLGDLAREEPICVVAEGLPPDALGADGRWCLLVDPIDGTR
                     GLMYQKRSAWILTGVAPNRGSATRLRDIVLAVQTEIPLLKQHLCDQLWAIRGEGA"
     gene            complement(474..1529)
                     /gene="galT"
                     /locus_tag="AC16JC_03825"
     CDS             complement(474..1529)
                     /gene="galT"
                     /locus_tag="AC16JC_03825"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007914524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HIR5163857.1"
                     /translation="MVWEQRWHPLRREWVIVSSHRNERPWHGERVIEMPRALPPYAPD
                     CYLCPGNLRSSGRRNDRYDGVFVFDNDHPCVGPDAPTALATPPGIYRNGPAAGCARVV
                     CYTPRHDLALAQLAEAEVRGLLEALQAQYRELGARNEVGHVLVFENRGEVTGTSNPHP
                     HCQIYATSFVFRTIELEAEAQSAHLAQQGRLLFQDILAAEEADGRRLLVRRDTALSFV
                     PYFARYPYETFVAPRAAHPSLATLTGPELADLAAVLRENLIRLDNLWRTSFPYVMVLH
                     QAPTKGSYPGFHFHIQIHPPLRKPGLLKYLAGPEIGGGNFLNDAAPEEKAAELRAVSS
                     VHYTQERDGGRGTEDEG"
     gene            1552..>2583
                     /gene="xylA"
                     /locus_tag="AC16JC_03830"
     CDS             1552..>2583
                     /gene="xylA"
                     /locus_tag="AC16JC_03830"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HIR5163858.1"
                     /translation="MDRRVKQDAYTPRPEHRFTFGLWTVGNPGRDPFGEPVRPPLAPA
                     HIVRTLAELGAYGVNLHDEDLVPRDAGAAERARIVREFRQVLGETGMKVPMATTNLFS
                     DPAFRDGAFTSNDARVRAYALQKTMAAIDLAVELGATTYVCWGGREGVETNAAKDPRT
                     ALGWYREAINYLCEYVRSQGYHLRFALEAKPNEPRGDIFLPTTGHMLAFIATLDHPEM
                     VGVNPEVAHERMAGLDFCHAVAQALEAGKLFHIDLNGQQAGRFDQDLRFGSDDPKGAF
                     FLVKLLEDAGWPGMRHFDSHAYRTEDEEGVWDFAAGSMRTYMILKEKVAQFNADPEIQ
                     RLRKELGGRG"
BASE COUNT          418 a          889 c          882 g          394 t
ORIGIN      
        1 acgccccctc cccccgaatc gcccatagct ggtcgcacag atgctgcttc agcagcggga
       61 tctcggtctg gaccgccagc acgatgtccc gcaagcgggt cgccgaaccg cgattcggcg
      121 cgacgccggt gagaatccac gcgctccgtt tctgatacat gagaccgcgc gtgccgtcga
      181 tcgggtccac gagcaggcac caccggccgt ctgctcccag cgcgtcgggg ggaaggcctt
      241 cggcgaccac gcagatgggc tcctctcgcg ccaggtcgcc gaggccctgc aggaagactt
      301 cctcggcaac gcggtcgacg gcgtaaatcg tgtcaccgcc tccggcggtc tcgtctgccg
      361 ccacggccgc gagctcgtcg gcagcctgcc gcgcgcacgc atcgaccacc gcagcgcgta
      421 cgcggtcgtg cagggagagc agaggctcga gcaggcgttc cgcgccagcc atctcagccc
      481 tcgtcctccg tcccccgtcc cccgtcccgc tcttgtgtgt aatgcacgga cgagacggcc
      541 cggagctccg cggccttctc ctcgggggcg gcatcgttca agaagttgcc gccgccgatc
      601 tccggtccgg cgagatattt caggaggccg ggcttgcgca gcggaggatg aatctggatg
      661 tgaaagtgga accccggata cgatcccttg gtgggcgcct ggtgcaacac catcacgtac
      721 gggaacgacg tgcgccacag gttgtcgagc cgtatgaggt tctcccgcag tacggctgcg
      781 aggtcggcga gctcgggccc cgtgagcgta gccaggctgg ggtgtgccgc gcgcggcgcg
      841 acgaacgtct cgtaggggta gcgggcgaaa tacgggacga acgacagcgc cgtgtcgcgc
      901 cgcaccagca gtcgccggcc gtccgcctcc tccgccgcga ggatgtcctg gaacagcagc
      961 cgtccctgct gagccaggtg cgcgctttgc gcctcggcct cgagctcgat cgtcctgaac
     1021 acgaagctcg tcgcgtagat ctggcagtgc gggtgcgggt tactcgtgcc ggtgacttcg
     1081 cccctgttct cgaacaccag cacgtgcccc acctcgttcc gcgcacccag ctcgcgatac
     1141 tgcgcctgga gggcttccag cagaccgcgc acctcggctt ccgccagctg agccagcgcg
     1201 agatcgtgcc gcggcgtata gcagacgacg cgggcgcaac cggcagccgg cccgttgcgg
     1261 tagattcccg gtggcgtcgc gagcgcggtg ggcgcatccg gcccgacgca cgggtgatcg
     1321 ttgtcgaaca cgaagacgcc gtcgtagcga tcattgcggc gccccgagct gcgcaaattg
     1381 ccgggacaga ggtagcagtc cggcgcgtag ggcgggagcg cgcggggcat ctcgatcacg
     1441 cgctcgccgt gccagggccg ctcgttgcgg tgggacgaca cgatgaccca ctcgcggcgc
     1501 aggggatgcc agcgctgttc ccacaccatc gtggcgccga gtatcggacc tatggatcgc
     1561 agagtcaagc aggacgccta caccccccgc ccggagcacc gcttcacctt cgggctctgg
     1621 acggtcggga atcccggccg cgacccgttc ggcgagccgg tgcggccgcc gctcgccccg
     1681 gcccacattg tccgcacgct ggccgagctt ggcgcgtacg gggtcaatct ccacgacgaa
     1741 gacctcgtgc cgcgggatgc gggcgcggcg gaacgcgccc gcatcgtgcg ggagttccgg
     1801 caggtgctcg gggagacggg tatgaaggtg ccgatggcga cgaccaatct cttctccgac
     1861 ccggccttcc gcgacggtgc gttcacgagc aacgacgccc gagtccgcgc gtacgcgctg
     1921 cagaagacga tggccgccat cgacctggcc gtggagctcg gcgcgacgac ctatgtctgt
     1981 tggggtggac gggaaggcgt cgagacgaac gcggccaagg atccccgcac cgcgctcggc
     2041 tggtaccggg aggccatcaa ctacctgtgc gagtacgtcc ggtcgcaggg ctaccacctg
     2101 cggttcgcgc tcgaggccaa gccgaacgag ccgcgcggcg acatcttcct gccgaccacc
     2161 ggccacatgc tcgccttcat cgccacgctc gatcaccccg agatggtggg cgtcaacccc
     2221 gaggtcgcgc acgagcggat ggcggggctc gatttctgcc atgccgtggc gcaggcgctc
     2281 gaagccggca aactcttcca catcgacttg aacgggcagc aggcagggcg tttcgaccag
     2341 gatctgcgct tcggtagcga cgacccgaag ggtgcgttct ttctcgtcaa gctgctcgag
     2401 gacgccgggt ggcccgggat gcgccacttc gacagccatg cgtaccgcac ggaagacgag
     2461 gagggagtct gggacttcgc cgccgggagc atgcggacgt atatgatcct caaggaaaag
     2521 gtcgcgcagt tcaacgccga tcccgagatc cagagactcc ggaaagagct ggggggccgg
     2581 ggt
//
LOCUS       DBNGZW010000156         4059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_151662, whole genome shotgun sequence.
ACCESSION   DBNGZW010000156 DBNGZW010000000
VERSION     DBNGZW010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_151662"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..818)
                     /locus_tag="AC16JC_03835"
     CDS             complement(<1..818)
                     /locus_tag="AC16JC_03835"
                     /EC_number="7.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002626654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0006119 - oxidative phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HIR5163859.1"
                     /translation="MSLADALAGMLVAALNAYVLTGGADFGGGVWDLLASGARQRAQR
                     DLVAQAIGPVWEANHVWLILAVVLLFTCFPPAFARLAVGLNLPLTGALVGIVLRGSAF
                     AFRSYGGDRDAVQRRWGRVFEAASLVTPILLGTAVGTVASGVIGEGGRGKGEGFVAGY
                     VATWLAPFPLAVGVFTLCCFAFLAAVYLTLEAPEGPLREDFRRRALLSGVALGVAAGL
                     TLVVAPPGASLMSSRLVGSAWALPFHLGTGMVAVGALGALWTRRYRLARVAAAA"
     gene            complement(815..2134)
                     /locus_tag="AC16JC_03840"
     CDS             complement(815..2134)
                     /locus_tag="AC16JC_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HIR5163860.1"
                     /translation="MSDLAAARVQMGMSLAFHIVFAVVGIAMPVLMVVAERRWRRSGD
                     PIHLDLAKRWARGTAILFAVGAVSGTVLSFELGLLWPGFMRFAGAIIGMPFSLEGFAF
                     FTEAIFLGVYLYGWERVSPRAHQLAGVMVAVSGALSGVFVVIANAWMNSPAGFDLVDG
                     RATNVQPIVAMANAAVIPEVLHMTLAAYAATGFAVAGIHAWLILRGRRAPFHRHALAI
                     ALLVGAPAAILQPLSGHLAATHVARHQPAKLAAMEALFETRAGAPVTLGGWPDASAGR
                     THFGLEVPYVLSLLAFDNPRAVVRGLDAVPRDEWPPLPAVHLSFQLMVACGTYMALVG
                     LWTAWLARKKVVLSARRGFLWSLVVAAPLGFVAIEAGWMVTELGRQPWVIYGVLRTAD
                     AVTPMPGQMWRTGVFALIYVFLAVVVTVLLSRQIVRSPKHTGEYKAP"
     gene            complement(2139..2978)
                     /locus_tag="AC16JC_03845"
     CDS             complement(2139..2978)
                     /locus_tag="AC16JC_03845"
                     /inference="COORDINATES: protein motif:HMM:NF021505.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HIR5163861.1"
                     /translation="MPDIAHFHPQIVHFAIVLLLAGVVMRWISLTGKVPFAGPAAATA
                     LLCGAVAAWLAATSGTQAHGPVERIPGVAAAVQAHEEWGERARTIALIVGGLELLALV
                     LIRVKPGLRRWAEIASAVVGLMAGYAVFEAAEKGGDLVYTYAGGPGLRSGDTADVGRL
                     LAAGLYGQAMVDRARHDSAGAAALIDELARRSPGDTAVALVRVESLLRDRGDARGALA
                     ALAALHVPADNPRVALRAGLLAVDAYLAVGQPDSARAVLRGLIAQHPMFQRLKDRLTQ
                     LGG"
     gene            3026..>4059
                     /locus_tag="AC16JC_03850"
     CDS             3026..>4059
                     /locus_tag="AC16JC_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014615.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HIR5163862.1"
                     /translation="MTPTLRDLIADHRVHLFDGAMGTMLYAKGFFLNVCYDELCIKQP
                     QLVREVHEEYVRAGAEIIETNTFGATPLKLGAHGLADQTREINRAAAQLARAAAGDRA
                     SVVGAIGPLGVRLEPFGPTSRDEARDLFRRQVDGLLEGGVDGFVLETFSDLEEAHAAL
                     RAVRDASDLASVVQMTIGTDGLTAYGTAPEDFARQLTAWGADVIGVNCSVGPAGVLDA
                     IEHMAKVTDVPLSAQPNAGLPRDVGDRKIYLASPEYMASYARRLVEAGARFVGGCCGT
                     TPEHIRKMRDYLASVTPRGSGVVVSRNEVTAPAGVSPVPMAARSHWGKKLACGEFVTS
                     VEIVPPRGVDP"
BASE COUNT          676 a         1514 c         1388 g          481 t
ORIGIN      
        1 tgcgccgccg ctgcgacgcg cgccaaccgg taacgccgcg tccataacgc gccgagcgcc
       61 ccgaccgcga ccattccggt cccgaggtga aacggcagcg cccaagcgga gcccaccagc
      121 cgactactca tcagcgacgc gcccggcggc gcgacgacca gcgtcaggcc ggccgcgaca
      181 cccagggcca cgcccgacag cagcgcgcgc cgccggaagt cctcgcgcaa gggcccctcc
      241 ggcgcctcga gcgtcagata cacggccgcg aggaacgcga agcaacacag cgtgaacacc
      301 ccgaccgcga gcgggaacgg cgcgagccac gtcgcgacgt agcccgcaac gaacccttcc
      361 cccttccctc ttcccccttc cccgatgacg cccgaggcca cggttcccac agcggtgccg
      421 aggaggatcg gcgtgacgag gctcgccgcc tcgaacacgc gtccccagcg gcgctgcacg
      481 gcgtcccgat ctccgccgta actgcggaac gcgaatgccg agccgcgcag cacgatgccc
      541 acgagcgccc ccgtgagcgg caaattgagt cccacggcca gccgcgcgaa agccggcggg
      601 aagcaggtga acagcagcac cacggcgagg atcagccaga cgtggttcgc ctcccacacc
      661 gggccgatcg cctgcgccac caggtcgcgc tgcgcacgct ggcgcgcacc gctggccagc
      721 aggtcccaca cgccgccgcc gaagtccgcg ccgcccgtga gcacgtaggc gttgagcgcc
      781 gccaccagca ttcccgcgag cgcgtcggcg aggctcatgg cgccttgtac tcgcccgtgt
      841 gcttcgggct gcgcacgatc tgccgcgaca gcagcaccgt cacgacgacc gcgaggaaca
      901 cgtagatcag cgcgaacacc ccggtccgcc acatctgtcc cggcatcggc gtcaccgcgt
      961 ccgcggtgcg cagcacgccg tagatcaccc acggctggcg gcccagctcg gtgaccatcc
     1021 agccggcttc gatcgcgacg aagccgagcg gtgcggccac gacgagcgac cacaggaagc
     1081 cgcgtcgcgc ggagagaacg actttcttgc gcgccagcca cgccgtccac agtcccacca
     1141 gcgccatgta ggtgccgcag gcgaccatca gctggaacga gaggtgcacg gccggcaacg
     1201 gcggccattc atcccgcggt accgcatcga gcccgcggac caccgcccgc gggttgtcga
     1261 aggcgagcag cgagagcacg taggggactt cgagaccgaa gtgcgtgcgc ccggccgacg
     1321 catcgggcca gccgcccagg gtcaccggag cgccggcccg ggtctcgaac aacgcctcca
     1381 tggcggcgag cttggcaggc tgatgtcgcg cgacgtgggt cgccgccagg tggcccgaga
     1441 gcggttgcag gatggccgcg ggcgcgccca cgagcaacgc gatggccagc gcgtgccgat
     1501 gaaacggcgc gcggcgcccc cgcaggatca gccacgcgtg gattccggcc acggcgaatc
     1561 cggtggcggc gtatgccgcg agcgtcatgt gcagcacttc ggggatcacc gcggcgttcg
     1621 ccatcgccac gatcggctgg acgttcgtcg cccggccgtc cacgaggtcg aacccggcgg
     1681 gagagttcat ccaggcgttg gcgatgacga cgaagacacc ggacagggcg ccgctcactg
     1741 ccaccatcac gcccgcgagc tgatgtgcgc gcgggctcac ccgctcccac ccatagaggt
     1801 agacgcccag gaagatggct tcggtgaaga acgcgaagcc ttcgagcgag aacggcatcc
     1861 cgatgatcgc gcccgcgaac cgcatgaacc ccggccagag caaaccgagc tcgaacgaca
     1921 gcaccgtgcc cgacacggcg cccaccgcga agaggatcgc cgtgccccgc gcccatcgtt
     1981 tggcgagatc caggtggatg ggatcgcccg agcggcgcca gcggcgctcg gccacgacca
     2041 tgaggaccgg catcgcgatc cccaccaccg cgaagacgat gtggaacgcg agcgacattc
     2101 ccatctggac gcgcgcggcg gccagatcgg acatgcggct acccgccgag ctgggtgagg
     2161 cggtccttga ggcgctggaa catcgggtgc tgcgcgatga ggccgcggag caccgcgcgc
     2221 gccgagtcgg gctgcccgac ggccaggtag gcgtcgacgg ccagcaggcc cgcccgcagc
     2281 gcgacccgcg ggttgtctgc aggcacgtgg agcgccgcga gggcggcgag cgcgccccgg
     2341 gcgtcgccgc ggtcgcgcag cagcgattcc acgcgcacca gcgccaccgc cgtgtcgccc
     2401 ggggagcgcc gtgccagctc gtcgatcaac gccgccgcgc cggccgagtc gtgccgggcg
     2461 cgatcgacca tcgcctgtcc atagagtccc gccgccagca ggcgtccgac gtcggccgtg
     2521 tcgccggagc gcaggccggg accgccggcg tacgtgtaga ccagatctcc acctttttct
     2581 gctgcctcga acaccgcgta cccggccatc agccccacca cggccgacgc gatctccgcc
     2641 cagcgccgca gcccgggctt gacccgtatc agcaccagcg ccagcagctc caatcctccc
     2701 acgatgaggg cgatggtgcg cgcgcgctcg ccccattcct catgcgcctg gacggccgcc
     2761 gcgacgccgg gaatccgttc caccggccca tgcgcctgcg tccccgacgt ggctgccagc
     2821 caggcggcga ccgcgccgca gagcagtgcc gtcgccgcag ccggtcccgc gaagggaacc
     2881 ttgccggtga gcgagatcca gcgcatcacc acccccgcca gcagcaggac gatggcgaaa
     2941 tggacgatct gcggatggaa atgggcgatg tcgggcatcg gaggaatcta gggagaatcc
     3001 gagtccgtgc tagattgagg gctccatgac tccgacgctg cgtgacctga tcgccgacca
     3061 tcgcgtccac ctgttcgacg gcgcgatggg gacgatgctg tacgccaagg gcttctttct
     3121 caacgtctgc tacgacgagc tgtgcatcaa gcagccgcag ctggtgcgcg aggtgcacga
     3181 ggagtacgtg cgcgccggcg cggagatcat cgagaccaac accttcggcg ccacgcccct
     3241 gaagctcggg gcccacggcc tggcagacca gacccgggag atcaaccgcg ccgccgcaca
     3301 gctcgcccgg gcggccgcgg gcgaccgcgc cagcgtggtc ggcgcgatcg ggccgctcgg
     3361 cgtccggctc gagccgtttg gcccgacgtc gcgcgacgaa gcccgcgatc tgttccgccg
     3421 ccaggtggat gggctgctgg aaggcggggt ggacggcttc gtgctggaaa cgttcagcga
     3481 cctcgaggag gcgcacgccg cgctgcgcgc ggtgcgcgac gcgtccgatc tggcgtcggt
     3541 cgtgcagatg accatcggca ccgacgggct caccgcctac ggcacggcgc ccgaggattt
     3601 cgcccgccag ctcaccgcgt ggggcgcgga cgtgatcggc gtgaactgct ccgtcggtcc
     3661 ggccggcgtg ctcgacgcca tcgagcacat ggcgaaagtc accgacgtcc ccctgtcggc
     3721 gcagcccaac gccggtctgc cgcgcgacgt gggcgaccgc aagatctatc tcgcctcgcc
     3781 ggaatacatg gcgagctacg cgcgacgcct ggtcgaggcc ggcgcgcggt tcgtgggcgg
     3841 ctgctgcggc acgacccccg agcacatccg caagatgcgc gactacctgg cgagcgtgac
     3901 cccgcgcggc tcgggcgtcg tcgtgtcgcg caacgaggtg accgctccgg ccggcgtctc
     3961 accggtcccg atggccgcgc ggtcccactg ggggaagaag ctcgcgtgcg gcgaattcgt
     4021 gacgagcgtc gagatcgtcc cgcccagagg cgtggaccc
//
LOCUS       DBNGZW010000157         2216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_419643, whole genome shotgun sequence.
ACCESSION   DBNGZW010000157 DBNGZW010000000
VERSION     DBNGZW010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_419643"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="AC16JC_03855"
     CDS             complement(<1..425)
                     /locus_tag="AC16JC_03855"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR5163863.1"
                     /translation="MNVIPQAVKDGFVRLTGPMVRALVRGHVRPNTITTIGALVVIGS
                     AVAYGMSAIRLGGLLLLLSGLFDILDGQVARQGGQSSAFGAFYDSSLDRVGESALFAG
                     IALHFLRGGLPAAQVALGVMLAIAALSASLMVSYTRARA"
     gene            complement(474..1706)
                     /locus_tag="AC16JC_03860"
     CDS             complement(474..1706)
                     /locus_tag="AC16JC_03860"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ family protein"
                     /protein_id="HIR5163864.1"
                     /translation="MRAHGLWVLGIVACSGPPSPAARVRPGIEVLLTDSIHLVRGRRL
                     GLLTNQTGADRAGRRDADAVRAAGLELVALFSPEHGFRGQEDRPGLPDAVDSATGLPI
                     YSLYGGSRAAARAALDSIDVLLIDLQDIGARYYTYPATATALMRDAAARGKAVVVLDR
                     PDPIGGASMAGDVRPAVGDPDSQLVGFLPVPMRHGMTLGELLRLANDVLALRADLVVV
                     PAAGWRRDMAYDVTGLPWIRPSPNMPDLESAFHYPGTCLFEGTNLSVGRGTPWAFQVI
                     GAPWLDAPRLLQRLQGAGSTEQSALNGVEVTATEFTPHAPTDGKYDGVPLHGLRFRVT
                     NRGRYDPTRLAVALLAGLVALHPDSFQFHPSSFDRLAAGPELRLAVSAGRTPVQVWDG
                     WSAALDQFRRTRLKYLLY"
     gene            1695..>2216
                     /locus_tag="AC16JC_03865"
     CDS             1695..>2216
                     /locus_tag="AC16JC_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR5163865.1"
                     /translation="MRSHRWIVLALIVACRPAPAGPGPAAPPAPFSPVSLDSVLSSLS
                     ASQKVGQLIVPWLSGAYTALDDSAFLEAARWVDSLHVGGIIISVGPPLEIAAKLNALQ
                     TRSALPLLVSADLEWGAAMRVRGATGFPQIMATGATGDPNDAYTIGAAAADEGRAVGI
                     HVNFAPDADLNNNP"
BASE COUNT          362 a          818 c          745 g          291 t
ORIGIN      
        1 tcggcccgcg cgcgggtgta ggacaccatc agcgatgccg acagcgccgc gatcgccagc
       61 atcacgccca gcgccacctg cgccgcgggc aggccgccgc gcaggaagtg gagcgcgatc
      121 ccggcgaaca acgccgattc acccacgcgg tcgagcgagc tgtcgtagaa cgccccgaac
      181 gccgagctct ggccgccctg ccgcgccacc tgcccgtcca gaatgtcgaa caggcccgac
      241 aacaggagca gcaggccgcc gagccggatc gcgctcatcc cgtaggcgac ggccgagccg
      301 atcacgacca gggccccgat cgtcgtgatc gtgttgggcc gcacgtgccc gcgcaccagg
      361 gctcgcacca tcggcccggt caggcgcacg aagccgtcct tcacggcttg cgggatcacg
      421 ttcatagccg ggtgaaacta acagcccgcg gaacgcccgc aaagggcaaa acgctagtaa
      481 agcaagtact tgaggcgcgt gcgacgaaat tgatcgagcg ccgccgacca gccgtcccat
      541 acctgcaccg gcgtgcggcc ggcgctcacc gcaagacgca actcgggccc ggccgcgagc
      601 cggtcgaaac tcgacgggtg gaactggaaa ctgtccggat gcagcgccac gagaccggcg
      661 agcagggcca ccgccagtcg cgtcgggtcg tagcggccgc ggttggtgac gcggaatcgc
      721 aacccgtgca gtggcacccc gtcatacttc ccgtcggtgg gggcgtgcgg tgtgaattcg
      781 gtcgcggtaa cctccacgcc attcagagcc gactgctccg tgctccctgc tccctggagc
      841 cgttggagaa gccggggagc atcgagccac ggcgccccga tcacctggaa cgcccacggc
      901 gtcccccgcc ccaccgagag gttcgtgccc tcgaacagac acgtgcccgg gtagtgaaag
      961 gcactctcga ggtcgggcat gttgggcgag ggtcggatcc agggcagccc ggtgacatcg
     1021 taggccatgt cgcggcgcca tcccgcggcc gggaccacca ccaggtcggc acggagcgcg
     1081 agcacgtcat tggccaggcg cagcagctcg ccgagcgtca ttccatgccg catcggcacc
     1141 gggaggaaac cgaccaattg actgtcggga tcacccacgg ccgggcgcac gtcgcccgcc
     1201 atcgatgccc cgccgatggg gtcggggcga tcgagcacca cgacggcctt gccacgggcg
     1261 gcggcgtcgc gcatcagggc ggtcgccgtg gccggatagg tgtagtaccg ggcgccgatg
     1321 tcctgcagat cgatgagcag cacgtcgatc gaatcaagag ccgcgcgtgc ggccgcgcgc
     1381 gagcccccat acaggctgta gattgggaga ccggtcgcgg aatccacggc gtccggcagg
     1441 ccgggacgat cctcctggcc ccggaatccg tgctccgggc tgaacagcgc caccagctcc
     1501 aggccggcgg cgcgcacggc gtcggcgtct cggcggccgg cgcggtcggc gcccgtctgg
     1561 ttcgtgagca ggccgaggcg gcgcccccgg acgaggtgga tggaatcggt cagcagcacc
     1621 tcgatgcccg gccgcacccg cgcagcgggg gacggtggac cgctgcaggc gactatcccc
     1681 aacacccaga ggccatgcgc tcgcatcgtt ggatcgtcct cgcgctgatc gtggcgtgcc
     1741 gccccgcccc cgccggtccg ggaccggcag cgccgcccgc gccgttctct cccgtgtcgc
     1801 tggacagcgt cctgtccagc ctgtccgcca gtcagaaggt cggccagctc atcgtcccct
     1861 ggctctcggg cgcctacacc gccctcgacg actcggcgtt tctcgaagcc gcccgctggg
     1921 tcgactcgct gcacgtcggg gggatcatca tctccgtcgg ccccccgctc gagatcgccg
     1981 ccaagcttaa cgcgctgcag acccgctcgg cgctgcccct gctcgtctcg gccgacctcg
     2041 aatggggcgc cgccatgcga gtccggggcg cgaccgggtt tccccagatc atggccacgg
     2101 gcgccacagg cgatccgaac gacgcctata ccatcggcgc cgcggcggcg gacgaaggcc
     2161 gcgccgtcgg catccacgtg aatttcgctc ctgacgccga tctcaacaac aacccg
//
LOCUS       DBNGZW010000158         1865 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_832753, whole genome shotgun sequence.
ACCESSION   DBNGZW010000158 DBNGZW010000000
VERSION     DBNGZW010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_832753"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="AC16JC_03870"
     CDS             complement(<1..335)
                     /locus_tag="AC16JC_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163866.1"
                     /translation="MNDVLRERMLRKLETLSEEKVYQVLDYVEFLESKYAERPAGAAP
                     FQKVAETLEDTMRAGRVPVTIIKGTMDAVGKAGKFFEGLAAAGKAAVDEVRKKSGDGT
                     PQPPPSPPAP"
     gene            complement(332..1369)
                     /locus_tag="AC16JC_03875"
     CDS             complement(332..1369)
                     /locus_tag="AC16JC_03875"
                     /inference="COORDINATES: protein motif:HMM:NF018095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR5163867.1"
                     /translation="MQSIRTLCVLPVVACLAVPLPAQARVRVARAGEWFYQAAEGKRL
                     AQLEAGAVVQQGTVQGDWAAVTLDGWIFKTSIGPTSVSGFDLAVTKAPSENLRTAPAG
                     SVVARLITGFALDRVGEQDRWVHVRRQGWMRRTALQAVAESAPSAPVSTNPPGGGRPP
                     RTTPADTGSTAAPIDASRVQVTRGTTVYRAPDSAAASDARVASGAPLRVLGRAGEWTR
                     VQLDGWVRTADVQAAPAGVLVGVSAAELRAEPERYVGQVLRWTLEIIALRTADDLRPD
                     IPDGSAYLLARGPVPERSFVYVVVPESKRAVVDALSPLATVQAMVRVRTGRSRFVGNP
                     VVDLVSLEVQP"
     gene            1380..>1865
                     /locus_tag="AC16JC_03880"
     CDS             1380..>1865
                     /locus_tag="AC16JC_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496618.1"
                     /note="catalyzes the interconversion of 2-phosphoglycerate
                     and 3-phosphoglycerate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HIR5163868.1"
                     /translation="MNKRHGSRTPVALIVLDGWGFRPGREGNAIELGDTPVWHRLWER
                     APRTLLDASGLAVGLPEGQMGNSEVGHLNLGAGRVVPQDIVRISQAITSGEFFDLPPL
                     VELCESVRRGKGTLHLLSLIGTGGVHALDEHLLAAVELGRRAGVPVAIHAWLDGRDTP
                     PQ"
BASE COUNT          262 a          698 c          631 g          274 t
ORIGIN      
        1 ggcgccgggg gggacggcgg gggctgcggc gtgccgtccc ccgatttctt gcgcacctcg
       61 tccacggcgg ccttccccgc ggcggccagg ccctcgaaga acttccccgc cttgcccacc
      121 gcgtccatcg tccccttgat gatggtcacc ggcacccggc cggcccgcat cgtgtcctcg
      181 agcgtctcgg cgactttctg aaacggcgcg gcccccgcgg gccgctcggc gtacttggac
      241 tcgaggaact cgacgtagtc gagcacctgg tagaccttct cctcggacag cgtctccagc
      301 ttgcgcagca tccgctcgcg cagcacgtcg ttcatggctg tacctcgagg gaaacgagat
      361 ccaccaccgg attgccgacg aaccggctgc ggccggtgcg cacccgcacc atggcctgca
      421 cggtggcgag cggcgacagg gcgtccacca cggcgcgctt cgactctggc accacgacat
      481 acacgaagct ccgctcgggg accggcccgc gggcgagcag gtaggccgag ccgtcgggga
      541 tgtcggggcg cagatcgtcc gcagtccgca gcgcgatgat ctccagcgtc cagcgcagca
      601 cctggccaac ataacgctcg ggctccgccc gcagctcggc cgcgctcacc ccgaccagga
      661 ctcccgctgg cgccgcctgg acgtccgccg tcctgaccca gccgtcgagc tgcacgcgcg
      721 tccactcccc ggcgcgtccc agcaccctga gcggggcgcc cgatgcgacc ctcgcatcgc
      781 tcgcggccgc gctgtcgggc gcgcgataca cggtcgtgcc gcgcgtcacc tgcacgcgtg
      841 acgcgtcgat cggggcggca gttgacccgg tatcggcggg cgtcgtccgg ggcgggcgcc
      901 ccccacccgg tggattcgtc gagaccggcg cggacggcgc actctcggca acggcctgga
      961 gcgccgtgcg gcgcatccat ccctggcgcc gcacgtgtac ccagcgatcc tgctccccga
     1021 ccctgtcgag cgcgaatccc gtgatcagac gtgccacgac gctgccggcg ggggccgtcc
     1081 gcaggttctc cgagggagcc ttcgtcacgg cgaggtcgaa gccgctcacg ctggtggggc
     1141 cgatcgaggt cttgaagatc cagccgtcca gcgtgacggc cgcccagtcg ccctggacgg
     1201 tgccctgctg caccaccgcg ccggcctcga gctgcgcgag ccgcttgccc tcggctgcct
     1261 ggtagaacca ctcgccggcc ctggcgacgc ggacccgtgc ttgagcgggc agcgggaccg
     1321 cgaggcaggc aacaactggt aggacgcaca gcgtgcggat agattgcatc cccttcaata
     1381 tgaacaagag gcacgggtcg cgcaccccgg tcgcactgat cgttctcgac ggctggggct
     1441 tccgccccgg ccgcgaaggc aatgccatcg agctcgggga cacgccggtc tggcaccgcc
     1501 tgtgggagcg tgccccgcgc accctgctcg atgcttcggg cctcgccgtc ggcctcccgg
     1561 aaggacagat gggcaacagc gaggtcggcc atctgaatct gggcgccggc cgcgttgtcc
     1621 cccaggatat cgtgcggatc tcacaggcca tcacctccgg cgaattcttc gatttgccgc
     1681 cgctcgtgga gctgtgcgaa tcggtgcgac ggggcaaggg cacgctccat ctcctgtcgc
     1741 tcattggaac gggcggcgtg cacgcgctcg acgagcacct gctggcggcc gtggagctcg
     1801 gccggcgcgc cggagtcccc gtggcgattc acgcgtggct cgatggacgg gatacaccgc
     1861 cccaa
//
LOCUS       DBNGZW010000159        15031 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_151870, whole genome shotgun sequence.
ACCESSION   DBNGZW010000159 DBNGZW010000000
VERSION     DBNGZW010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 15031)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 15031)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15031
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_151870"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..983)
                     /locus_tag="AC16JC_03885"
     CDS             complement(153..983)
                     /locus_tag="AC16JC_03885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalate:formate antiporter"
                     /protein_id="HIR5163869.1"
                     /translation="MTEFLLDESAWPTTLTEAHRERLRSVRDGLRLAQELLGLAAGGS
                     FIAGEMDAYSDLDLVAVVAPSAWPGVLDRRGAMAARLGPLLAAFTGEHVGEPRLLICL
                     YGPPLVHVDLKFVHLEALAERVEDPVLLWDRTGDVRRRLDATPARYPQPNLQWIEDRF
                     WIWVHYATAKLARGELFEALNMIAYLRDRVLGPLALLEAAARPNGLRRIETLAPGRIP
                     ALRATLAGLDATEIRRALEAAIAGYRDLRTVLAPPGLVRSAAEPPAVAFLGEVAERIR
                     "
     gene            1053..3665
                     /locus_tag="AC16JC_03890"
     CDS             1053..3665
                     /locus_tag="AC16JC_03890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007843725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR5163870.1"
                     /translation="MPFAFRAWSILLILSGRAAAQGARPLSRDSTRPAAERARDLLAR
                     MTPEEKFWQLFMIPGDLDDSAHDYSHGIFGLQVGTSDTSPPAEAARADARRINAIQRY
                     FVERTRFGIPIIPFEEAVHGLRRPGGTMFPQAIALAATWDTALVGRVADAIAREARSR
                     GIRQVLSPVLNLARDVRWGRVEETYGEDPYLASAMGRAFVGFLERAGIVTTPKHFVAN
                     VGDGGRDSYPIDYDERLLAETYYPPFESAIRQAGARSVMTAYNSVDGAPATQNRRLLT
                     EVLKRRWGFPGFVISDAAATGGATVLHMTEPSTAAAAKDALEAGLDVIFQSAYEQYRP
                     YWDAFRNGTIPPAVIDSAVARVLRVKFSLGLFEHPYVNPDSARFWNGHTAHRALAREA
                     AQEGIVLLRNVHGLLPLSDTLRSLAVIGSDAVEARLGGYSGPGNRPIAMLDALRARLG
                     AQRVGFAPGPGRVSPDAVTVPADALSPGLNGEYFDNIALEGRPLFVHHDERIDFGWTL
                     SSPGPGLARDWYSVRWTGTLTVPAGGARHIGVAGSDGFRLFLDDSLLIDDWQKRSAGT
                     YLADVALAGGSAHALRLEFFETTGNAHVRLVWDAGVVDDWRAQVDSAVRLARRSEVAV
                     VVAGVEEGEFRDRAFLGLPGHQEELIREVAATGTPVVVVLVGGSAVTMPWLDRVDGVL
                     DAWYPGEAGGQAVADVLFGDVDPAGRLPVTFPIAEGQLPLVYDHKPTGRGDDYLDLTG
                     QPLFPFGFGLSYTAFAYSGLVIEPAAIRPGGSAVVRCRVKNVGGRAGDEVVQLYLRDE
                     LASVPRPVLQLAGFQRVRLAPGEEREITFRLGPEQLRLLGRDLRWVVEPGTFRVLLGG
                     SSKDIRLRGALRVQ"
     gene            complement(3791..4444)
                     /locus_tag="AC16JC_03895"
     CDS             complement(3791..4444)
                     /locus_tag="AC16JC_03895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013630782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5163871.1"
                     /translation="MRRPVIVLAILGSVLVSCKKRPEQAATPPATATSLNPASPQMKA
                     TAPTTYRARFETSAGIFTVEVTRAWAPLGADRFYNLVRNGFYDGGRFFRVLPGFVVQF
                     GLPGDPAVAAQWRPATIPDDPVVQHNTRGTLTFATGGPATRTTQVFINYGDNVQLDGM
                     GFSPLGRVVEGMDVVDRIYAGYGEQPDQGMIQMRGNAYLATQFPRLDSIAHATIVTP"
     gene            complement(4503..5369)
                     /locus_tag="AC16JC_03900"
     CDS             complement(4503..5369)
                     /locus_tag="AC16JC_03900"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007261454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR5163872.1"
                     /translation="MSRDSDYSRTINHHYGVADLPARILDRLRAAGKDPATVTRDDLA
                     MFDEFHTGGRESTRDLARLAGIRPGMRVLDVGSGIGGPARTLAAEFGAAVVGLDLTEE
                     FCRAATVLTELVGLADRVTFRHGDALALPFPDGRFDAVWSQNSIMNIPDHGRLFAEMH
                     RVLVPGGTLALEAVFAGPVEGVIFPTFWASAPELNFLMAPDAARALLGTVGFHEVLWE
                     DTTADTLDHARRRRPPPAGPEAAPALGRDTIVLEDVAGKIENAVRNLEGNRIATVRAV
                     LRRSGPSTRARC"
     gene            complement(5414..6058)
                     /locus_tag="AC16JC_03905"
     CDS             complement(5414..6058)
                     /locus_tag="AC16JC_03905"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5163873.1"
                     /translation="MSHVLALLTLLALNFGGWAVITVENLPDQVVVGQPMTLDFTVRQ
                     HGRTLLSNLNARVVAEGGGDRVSAAAQPGGSEGRYRATVTLPHAGAWTITIYSGFGNS
                     NVTLLPLMAVGPGRSVAALAAAERGRQLFVAKGCLTCHVHPEVPGSGVVKVGPDLTGR
                     RFTGDYLQRFLADPNIATTRGPGGFEMPNLNLAPREIAALAAFINGETAAQTGQ"
     gene            complement(6259..6570)
                     /locus_tag="AC16JC_03910"
     CDS             complement(6259..6570)
                     /locus_tag="AC16JC_03910"
                     /inference="COORDINATES: protein motif:HMM:NF015582.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HIR5163874.1"
                     /translation="MTTTPQSRHGPTMQLYIMVWIGLLCIVGAEVLLAYAHLRTGVLL
                     AGLLALALVEAAAGLLFFMHLKYERAKLAWWLIPVLIFVFLLLDHIWPDAFRLAALRA
                     P"
     gene            complement(6567..7184)
                     /locus_tag="AC16JC_03915"
     CDS             complement(6567..7184)
                     /locus_tag="AC16JC_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011687277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR5163875.1"
                     /translation="MAESVMTAHPAGEESPFGIQSKKLAIWLFIAADSATFGAILFAY
                     GFLRVGAADWSHPFAFFPSILNGIVMTVVLLTSSLTMLAAVAAAQRGDIPKTVRWMGI
                     TALLGATFAALHLREWLNMLHEGWSPSKNPLGGPAQFGATFFGITGLHLLHVTCGVLA
                     IVVIALLVRRGKLTAGHVETTGLYWHFVDLVWMFVFPLVYLMNAR"
     gene            complement(7201..7752)
                     /locus_tag="AC16JC_03920"
     CDS             complement(7201..7752)
                     /locus_tag="AC16JC_03920"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR5163876.1"
                     /translation="MSVQVDRQALTTPARTGVWIGIAAIVLMVAAFTGALVVRRTQSD
                     WIHFRLPPIIYVNTLVLLVSSATLERARGGAGAAPQGRWLSITLGLGALFVVGQVLAW
                     SNLLAQGLFLATSPSSAFFFVLTALHGVHLLGGVAALAYVLGKARHGPGSGAAGALDA
                     AALYWHFLAVLWLYLLLLLTLQL"
     gene            complement(7749..9530)
                     /locus_tag="AC16JC_03925"
     CDS             complement(7749..9530)
                     /locus_tag="AC16JC_03925"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR5163877.1"
                     /translation="MSHPATAAPAAAHAHGPPQSFLRKHVFSLDHKVIGKQYLGLGLV
                     AVCTGMVLSWLMRLHLANPDLALPLIGKLWPNGAPGGVMTPEFYLSLMTMHGTIMVFF
                     VLTTAPFAGFGNFALPIQIGAEDMAFPRFNMMSFWVTFLAFLVLVAAFFVPAGPPISG
                     WTAYAPLSAVGKDAGPGQGTGQLLWAISIAIFCVGQLLGSLNFIATTLDLRTKGMTLA
                     RLPYSTWAWFITAVIGLIAFSVLLPACILLILDHVAGTSFFIPAGLVISDTLQPHSGG
                     SPLLWQHLFWFFGHPEVYIAILPGFGIVSQILPVFARKPLLGPRVLIACMIAIGFLSY
                     MVWGHHMFLSGMNPFSATLFSVPTLVITIPATIITLLWIGTLYGGKLRFTTSMLFCIG
                     FVSVFVSGGISGFFLAQPSIDVYLHGTYFVVGHFHFVMGVAAMFGIFAGTYYWFPKMF
                     GRMMNETLGKIHFALTFVGVYAIFMPMHYLGLHGDVRRYSAFTDEYMKGSIPLHRFIT
                     VAALFTGAVQLIFLYNLVRSRFRGAPAPDNPWRATTLEWTTSSPPPHDNFGGHHPVVT
                     HGPNEYGAAGEADDHIMQTAPERRAGA"
     gene            complement(9527..10330)
                     /locus_tag="AC16JC_03930"
     CDS             complement(9527..10330)
                     /locus_tag="AC16JC_03930"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HIR5163878.1"
                     /translation="MGKLFAVLLAIITIVSTTIFIRHTWWMPPDYSLHGIRIDHQLAE
                     TMTASGILFVLAQLVLAGFVWQYGDRKEAPAVKRFPGGATPMVVLASILVGIEILTLT
                     LVGSKVWAGIYQAPPDPGSLHIDVQAEQFAFYFRYAGADGVFGGTHPELIDPASGNFF
                     GLDPANDTTARDDIVSGSLAIPVNQPIQLSMHAKDVGHAFYVPELRIQQDFVPGLVIP
                     LHFTATRTGKFELVCTQLCGLGHYNMKAYVEVMAPEQFAQWLKDQAAQQ"
     gene            complement(10347..11075)
                     /locus_tag="AC16JC_03935"
     CDS             complement(10347..11075)
                     /locus_tag="AC16JC_03935"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR5163879.1"
                     /translation="MNRHFAPAIGVLALAGLAAAATDAPAARPAAAGTISGTVTFTGT
                     PPKMRPIDMSKEPSCAKQHTTPVMTENVVTGPGSTVEYVVVYVSAGGQPGAAPTQAVR
                     YDQKGCQYVPHVAVLQTGQPLDIYNNDQTSHNIHPLAKVNPEWNKSQPPGTPPIHATY
                     DKPEFIAVKCNVHPWMHGYFAVLTTSHYAVTGNDGKFSLAGLPAGKYTVTAWHERFGT
                     QSQEVTVSEGGIATANFVFKALPY"
     gene            complement(11072..11920)
                     /locus_tag="AC16JC_03940"
     CDS             complement(11072..11920)
                     /locus_tag="AC16JC_03940"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5163880.1"
                     /translation="MRPQSRWIPLVSAWGAVLCLGGVTPLVAQATHIANVTGQAARGA
                     RLYQRYCVGCHGQDGDGNGENAMWIDPKPRDFTAAVFKCRSTPSGNLPTDQDLYDAVT
                     RGFVTTNMPPWVALTPQNRVDLVAYIKTFSPKWSGAPAPTPITIPTETPATIESILRG
                     RQTFQRLECWKCHGPAGHGDGPSASTLTDSKDNPIRPYNFSSGTRFKCGATNQDLYRI
                     FMTGLDGTPMPSFIDNLQPNEAWDLVHFLRTLQPVKSPEALTWQSWLQTHRGELKPIG
                     PEGGTQ"
     gene            complement(11936..13372)
                     /locus_tag="AC16JC_03945"
     CDS             complement(11936..13372)
                     /locus_tag="AC16JC_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163881.1"
                     /translation="MVERLTKGLVAAILLCTVGAAPGSGSPAAAPGTAIAFRTAEMAA
                     TYTAYTRSVARALHARAAIPAFARKYGLPCSACHTVWPELSAFGQRFKDNGYQLGNDR
                     DSPIWQSNAYWPVAIRTTPQWHAENTTHQVTDAVPAGKTVSQTGFDLSGIDFLFLGTL
                     YKNVTFGLVPTLDADGTTGVEAAFVRFDNLGNSPWANVKVGKFELDNLLSEKRFTWLS
                     NNGGFLYAYHYEPAGSVDSYAFGLGDNQIGAELSGHSVNSYTRYSVSLLTTDDGEAGL
                     PGGKSMDAMVSLSQAWAMGSGLGPTRIGLFGYLGRRPTAIETIGGEPIPGTGSANKSF
                     VRIGATAQVWLGNLELLPMVTHAQDDAALGGSTEKPAWNTALLEAHYVATPRLMVQGR
                     YELLRMSKQADPATPKSFGNADAFALGFRAYPFLFSRDGMAIHGEFALTNTTGMAPLS
                     GDGSGVDAVDPATKIRSLSFLLAFDFAF"
     gene            complement(13518..14018)
                     /locus_tag="AC16JC_03950"
     CDS             complement(13518..14018)
                     /locus_tag="AC16JC_03950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163882.1"
                     /translation="MTDERIAKLHALLSAYAERQAAAKPASRAGDSTAERRRRACADL
                     LRAVVRPVLERFMEELKKAGHDASTRDHTDRDDAYPSVALSFTPRSVAAAKSPEAVLA
                     SALMFKYDPRRGIVVQRDVKLSPAKGRVAPATGDRTGTIGVDAVSAEWVATKALSFVE
                     DVLKAN"
     gene            complement(14038..14616)
                     /locus_tag="AC16JC_03955"
     CDS             complement(14038..14616)
                     /locus_tag="AC16JC_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5163883.1"
                     /translation="MSAREFRALEPEARSRSGRPQPGEYAAYAHEEIAAIPEDDAVAA
                     LRSSRDRVMALFSALTDARVTGVRYAPDKWTIKDLLGHLSDDERIYAYRALRLARGDD
                     GVLAGFDQVRYADAAGSEARSLPDLLEEYRSVRTASITLFAGLGPEAWTRRGTLEGHD
                     VTVRGLAFHIVGHEGHHLRVLRDRYLPLVSPT"
     gene            14676..>15031
                     /locus_tag="AC16JC_03960"
     CDS             14676..>15031
                     /locus_tag="AC16JC_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5163884.1"
                     /translation="MKLDARTVALVLMGAPGLLAAQSAASPVADAFRTYEARSARKLV
                     AAIEAMPADKLGYKPTPAQMSVAAIALHLAEGNDYFCSTISGTPAPKRDSVTASSSKE
                     ALVARLRETFDFCQTTL"
BASE COUNT         2392 a         4975 c         5352 g         2312 t
ORIGIN      
        1 cggcgagcca tccggcggcg agaccgatca ccaggaacca gatgagattc gtgccctgca
       61 tgatccctcc tctgttggtg acgtggatca atctcccaac gtaccgtaca ccgtggaacc
      121 tcgcgaggac cccttcgcgg ggctttggga catcacctga tccgctcggc cacctccccg
      181 aggaacgcga cggcgggcgg ttccgcggcg gaccggacca ggccgggagg ggcgagcacg
      241 gtgcgcaggt cgcgataacc ggcgatggcc gcctcgaggg cgcgccggat ctcggtcgca
      301 tcgagtcccg cgagcgtcgc ccggagcgcg ggaattcgtc ccggcgcgag cgtctcgatg
      361 cggcgcaggc cgttcgggcg ggccgcggcc tcgagcagcg cgagcggtcc gagcacgcgg
      421 tcgcgcagat aggcgatcat gttcagcgcc tcgaacagct ccccccgcgc cagtttggcc
      481 gtcgcgtagt gaacccagat ccagaaacgg tcctcgatcc actgcagatt cggctgcggg
      541 tatcgcgccg gggtggcgtc gagccgccgt cgcacgtccc cggtccggtc ccacaacagc
      601 acggggtctt cgacccgttc cgcgagggcc tcgagatgca cgaacttcag atcgacgtgc
      661 accaagggcg gtccgtacag gcagatcagc agccgcggct cgccgacgtg ctccccggtg
      721 aacgccgcga gcagcggtcc caggcgcgcg gccatcgccc ctcgccggtc gagcacaccg
      781 ggccaggccg acggcgcgac gaccgccacc agatcgagat cggaatacgc gtccatctct
      841 cccgcgatga aggagccgcc ggccgcgagc cccagcagtt cctgcgcgag ccgcaatccg
      901 tcacgtacgc tgcgcagccg ctcccggtgc gcctccgtga gcgtcgtggg ccacgcggat
      961 tcgtcgagca ggaactccgt cattcgtcga agcctggcat gggggcaaaa catagctcgc
     1021 gcccggagcg gagcgcacgg tatcgttggg ccatgccctt cgcctttcgc gcctggtcga
     1081 tcctgctcat cctctcggga cgcgcggccg cgcagggggc gcgcccgctc tcccgcgatt
     1141 ccacccggcc cgccgcggaa cgcgcccggg acctgcttgc ccgcatgacg ccggaggaga
     1201 agttctggca gctgttcatg atccccggcg acctggacga ttcggcgcac gactactccc
     1261 acgggatctt cgggctgcag gtcggcacca gcgacacctc cccccccgcc gaagcggcac
     1321 gcgccgacgc ccggcgcatc aacgccatcc agcgctattt cgtcgagcgc acccgcttcg
     1381 gcatcccgat catcccgttc gaggaagccg tccatgggct gcggcgcccg ggcgggacga
     1441 tgttcccgca ggccatcgcc ctggccgcga catgggacac ggcgctcgtc ggccgcgtcg
     1501 ccgacgcgat tgctcgtgag gcgaggagtc gcgggatccg ccaggttctg tcgcccgtgt
     1561 tgaacctggc ccgtgacgtg cgctgggggc gggtagagga gacctacgga gaggatccct
     1621 acctcgcgtc cgccatggga cgagcgttcg tcgggttcct ggagcgggcg gggatcgtca
     1681 cgacccccaa gcacttcgtc gccaacgtcg gggacggagg tcgcgacagc tacccgatcg
     1741 actacgacga gcggctgctc gccgagacgt actatcctcc cttcgagtcg gcgatccggc
     1801 aggccggcgc ccgctcggtc atgacggcct acaactcggt ggacggcgcg cccgccaccc
     1861 agaaccgccg gctgttgacc gaggtcctga agcgccgctg gggattcccg ggattcgtga
     1921 tctccgacgc cgccgcgacc ggaggagcca cggtgctgca catgaccgag cccagcacgg
     1981 ccgcggccgc caaagacgcg ctcgaggccg ggctcgatgt gatcttccag tccgcctacg
     2041 agcagtaccg tccgtactgg gacgcgttcc ggaacggcac gatcccgccg gctgtgatcg
     2101 attcggcggt ggcgcgcgtg ctgcgcgtga aattctcgct gggactgttc gagcatccct
     2161 acgtgaaccc cgacagcgcg cggttctgga acggccacac cgcgcaccgc gcgctggcgc
     2221 gcgaggcggc gcaggaaggg atcgtgctgc tccgcaacgt gcatggcctg ttgccgctga
     2281 gcgacacgct ccgctcgctg gcggtgatcg ggtccgatgc ggtcgaagcc cggctgggcg
     2341 gctacagcgg gcccggcaac cgccccatcg cgatgctcga cgcgctccgc gcgagactcg
     2401 gcgcgcagcg ggtcggcttt gcaccgggcc cgggccgggt gagccctgac gcggtcacgg
     2461 tcccggcgga cgccctgagt ccgggactga acggggagta cttcgacaac atcgcgttgg
     2521 aggggcgccc cctgttcgtc catcacgacg agcggatcga cttcggatgg acgctttcat
     2581 cccccggacc gggtctggcc cgggactggt actcggtccg ctggaccggc acgctcaccg
     2641 tgccggcggg aggcgcccgg catatcggag tcgcgggcag cgacggcttt cggctcttcc
     2701 tggacgacag cctgctgatc gacgactggc agaagcggtc ggccgggacg tatctggccg
     2761 atgtcgcgct cgccgggggc agcgcgcacg cgctgcggct cgagttcttc gaaacgacgg
     2821 gcaacgccca cgtccggctg gtgtgggacg cgggcgtggt ggacgactgg cgcgcgcagg
     2881 tggacagcgc ggtgcgtctg gcacggcgga gcgaggtcgc ggtcgtcgtc gctggtgtcg
     2941 aagagggaga gttccgcgat cgggccttcc tcggtttgcc ggggcaccag gaggagctga
     3001 tccgggaggt cgccgccacc ggtacgcccg tcgtggtggt cctcgtcggc ggcagcgccg
     3061 tgacgatgcc gtggctcgac cgggtggacg gggtgctcga cgcctggtac ccgggtgagg
     3121 cgggagggca ggcggttgcc gacgtgctgt tcggggacgt cgatcccgcc ggacggctcc
     3181 ccgtcacctt tcccatcgcg gaaggtcagc tgccgctcgt ctacgatcac aagccgaccg
     3241 gccgggggga cgactatctc gatctcactg ggcagccgct ctttccgttc gggttcggac
     3301 tctcctacac ggcgttcgcc tactcggggc tcgtgatcga gccggctgcg atccggccgg
     3361 gggggagcgc ggtggtgcgg tgccgcgtga agaacgtcgg gggaagggcg ggcgacgaag
     3421 tggtgcagct ctatctccgc gacgagctgg cctcggtccc gcgcccggtg cttcagctcg
     3481 cggggtttca gcgggtacga ctcgctccgg gggaggagcg ggagatcacg ttccggctgg
     3541 gacccgagca gctgcggttg ctcggccgcg acctccggtg ggtggtggag ccggggacgt
     3601 ttcgcgtcct gctcggcggc tcctcgaagg acatccggtt gcggggcgcc ctcagggtgc
     3661 agtgaaggca gggagcgggc gggcggtcag tcccgccacc agatggcgca gcgccacctg
     3721 ccagacgagc ggctgcccgg ggagatcgtc gcggcgcgcc gtccggatct ccgcgcgcga
     3781 ccaacccttc ctacggtgta acgatcgtcg cgtgcgcgat ggaatcgagc cgcgggaact
     3841 gggtggccag gtacgcgttg ccgcgcatct ggatcattcc ctgatcgggt tgctcgccgt
     3901 agcccgcgta gatgcggtcc acgacgtcca tcccctccac cacgcgtccc aggggcgaga
     3961 atcccatgcc gtccagctgc acgttgtcgc catagttgat gaacacctgc gtcgtgcgcg
     4021 tggccggtcc cccggtcgcg aaggtgaggg tgccgcgggt gttgtgctgt accaccgggt
     4081 cgtcggggat cgtggccggg cgccactgcg ccgcgacggc cgggtcgccc ggcaggccga
     4141 actgcaccac gaacccgggc aggacgcgga agaaccgccc gccgtcgtag aacccgttcc
     4201 gcaccaggtt gtagaaccgg tcggcgccca gcggcgccca ggctcgggtc acctcgaccg
     4261 tgaaaatccc cgcactcgtc tcgaagcgtg cgcggtaggt ggtgggtgcc gtggccttca
     4321 tctgggggga ggccggattg agcgatgtgg cggttgccgg aggagtggcg gcctgctccg
     4381 ggcgtttctt gcaggaaacc aatacgctcc ccaggatggc gagcacgatc accgggcgtc
     4441 gcatgcgccg aaatcctcgc tgtcagggtg ggactgccgg tgttgccgat cggaaaatta
     4501 catcagcaac gggctcgggt ggacgggccg ctgcggcgca ggacggctcg gaccgtcgcg
     4561 atgcggttgc cctccaagtt gcgcaccgcg ttctcgatct tccccgcgac gtcctcgagc
     4621 acgatggtgt cacgtcccag ggccggggcc gcctcgggac ccgccggggg tgggcggcgg
     4681 cggcgggcgt gatcgagggt gtccgcggtg gtgtcctccc agagcacctc gtggaacccc
     4741 accgtcccca gcagcgcgcg ggccgcatcc ggggccatga ggaagttcag ctccggcgcc
     4801 gaggcccaga atgtcgggaa gatgacgccc tcgaccggac cggcgaacac ggcctcgagg
     4861 gcgagcgtgc cgcccggcac taggacgcgg tgcatctcgg cgaacaggcg gccgtggtcg
     4921 gggatgttca tgatgctgtt ctgcgaccac accgcgtcga accggccgtc cgggaacggc
     4981 agcgcgagcg cgtcgccgtg ccggaacgtc acccggtcgg cgaggcccac gagctcggtc
     5041 agaacggtgg cggcgcggca gaactcctcg gtgaggtcga gtcctacgac cgccgcgccg
     5101 aactcggccg cgagcgtgcg cgccggcccg ccaatcccgc tgccgacgtc cagcacccgc
     5161 atgccgggcc ggatgccggc gagacgggcc agatcgcgcg tggactcccg cccgccggtg
     5221 tgaaactcgt cgaacatcgc gagatcgtcg cgcgtgaccg tcgcggggtc cttgccggcg
     5281 gcgcggagcc ggtcgaggat ccgcgccggc aggtcggcga ctccgtaatg gtggttgatg
     5341 gtgcgcgagt agtcgctgtc ccggctcatc tggcacaagt tgccgggaca gcgacacggg
     5401 ttcaagcccg ggcttactgc cccgtctgcg ccgccgtctc cccgttgatg aacgcggcca
     5461 gcgcggcgat ctcccgcggc gcgaggttca gattgggcat ctcgaaccca cccggcccgc
     5521 gcgtggtggc gatgttcggg tccgccagga accgttgcag gtagtctccg gtgaatcgcc
     5581 gcccggtcag atccggcccc accttgacga ctccgctgcc cggcacctcc gggtggacgt
     5641 gacaggtgag gcaccccttc gccacgaaga gctgccggcc ccgctcggcc gcggcgagcg
     5701 ccgccaccga ccgcccggga cccaccgcca tcaacggcag cagggtgaca ttgctgttac
     5761 cgaatccgga gtagatcgtg atcgtccaag cgccggcgtg cggcagcgtg acggtcgcgc
     5821 ggtaccggcc ctccgaacca cctggttgcg cagcggcgct cacccggtcg ccgccgcctt
     5881 ccgcaaccac ccgggcgttc aggttggaga ggagcgtgcg cccgtgctgg cgcaccgtga
     5941 agtcgagcgt catgggttgc cccacgacga cctgatccgg caggttctcc accgtaatga
     6001 ccgcccagcc gccgaagttg agggcgagca gggtgagcag cgcgagtacg tgtgacatcc
     6061 gatcctccgt cgaggtttgg aggttggatg ccgccgcgcg actccgggtt tggagcctcc
     6121 gggagggctc agtcccggga gctcttgtcc cgtctgcgat aggccatgag ggcgatgccg
     6181 gcgaccacca gaaaggacac caccgtcagc aggaccatgc cgcccaggag ggaggccatg
     6241 ggatgatcca caaggctcct agggagcccg cagcgcggcc aaccggaacg cgtcgggcca
     6301 gatgtggtcc agtaggagga acacgaagat cagcaccggg atcaaccacc aggccagctt
     6361 ggcgcgctcg tacttcaggt gcatgaagaa caacaggccg gcggcggcct cgaccagtgc
     6421 cagggccagc aggcccgcga gcagcacgcc cgtgcgcaga tgggcgtagg ccagcagcac
     6481 ttcggcgcct acgatgcaga gcaggccgat ccataccatg atgtagagct gcatggtcgg
     6541 tccgtgccgg gattgcggag tcgtggtcat cgggcgttca tcaggtagac gagggggaac
     6601 acgaacatcc agaccaggtc cacgaagtgc cagtacaggc cggtggtctc cacgtggccc
     6661 gccgtcaact tgccgcggcg gacgagcagt gcgatcacca cgatggcgag cacgccgcag
     6721 gtcacgtgca gcaggtggag ccccgtgatc ccgaagaatg tggcgccgaa ctgggcgggg
     6781 ccgcccagcg gattcttgga cgggctccag ccctcgtgga gcatgttgag ccactcgcgc
     6841 aggtgcagcg cggcgaacgt cgcaccgagc agcgccgtga ttcccatcca gcgcacggtc
     6901 ttcgggatat ctccgcgctg ggcggcggcg acggcggcca gcatggtcag gctgctggtg
     6961 agcagcacga cggtcatcac gatgccgttc aggatgctgg gaaagaacgc gaacgggtgg
     7021 gaccagtcgg ccgctcccac gcggaggaat ccgtaggcga acaggatcgc cccgaaggta
     7081 gcggagtccg cggcgatgaa cagccagatc gcgagcttct tcgactggat cccgaagggg
     7141 gattcttccc ccgccgggtg cgcggtcatc accgattcgg ccacgttctc tccccgcggg
     7201 ttagagctgc agtgtcagga gcagcagcag atagagccac agcacggcca ggaagtgcca
     7261 gtacaacgct gccgcgtcga gcgcaccggc cgcgccgctc cccggcccgt gccgggcctt
     7321 ccccagcacg taggccagcg cggccacgcc gcccagcagg tggacgccgt ggagtgcggt
     7381 gagcacgaag aagaaggcgc tggacgggct ggtggcgaga aacaggccct gggcgaggag
     7441 gttgctccag gccagcacct gacccaccac gaagagcgca cccagcccca gcgtgatgga
     7501 cagccagcgg ccctggggcg cggcgccggc tcctccgcgg gcgcgctcca gcgtggcgct
     7561 gctcacgagc agcacgagcg tgttcacgta gatgatcggc ggcaggcgga agtggatcca
     7621 gtccgattgg gttcgccgga ccacgagcgc accggtgaat gcggcgacca tcaggacgat
     7681 ggctgcgatc ccgatccaga cgccggtgcg cgccggcgtc gtgagggcct gccggtcgac
     7741 ctggacgctc atgcgccggc ccgccgctcc ggcgccgtct gcatgatgtg gtcatccgcc
     7801 tcaccggccg cgccgtactc gttcgggcca tgggtgacca ccgggtggtg gccgccgaag
     7861 ttgtcgtgcg gcggaggcga ggaggtggtc cactcgagcg tggtggcccg ccacgggttg
     7921 tcgggggccg gggcgccacg gaagcggctg cgcaccaggt tgtagaggaa gatcagctgc
     7981 accgcccccg tgaacagggc ggcgaccgtg atgaagcggt gcagcgggat cgagcccttc
     8041 atgtactcgt cggtgaaggc cgagtagcgt cgcacgtcgc cgtgcaggcc gagatagtgc
     8101 atcggcatga agatggcgta cacgccgacg aacgtgaggg cgaagtggat cttgccgagc
     8161 gtctcgttca tcatgcggcc gaacattttc gggaaccagt agtaggtccc ggcgaagatc
     8221 ccgaacatgg cggcgacgcc catgacgaaa tggaagtggc ccaccacgaa gtaggtgccg
     8281 tgcaggtaca cgtcgatcga gggctgcgcc aggaagaacc cgctgatgcc gccgctgacg
     8341 aagaccgaga cgaagccgat gcagaacagc atcgacgtcg tgaagcgcag cttcccgccg
     8401 tacagcgtcc cgatccacag cagcgtgatg atcgtcgccg gaatcgtgat gaccagggtc
     8461 gggacggaga acagcgtcgc cgagaagggg ttcatgccgc tgaggaacat gtggtggccc
     8521 cacaccatgt agctcaggaa gccgatcgcg atcatgcagg cgatcaggac gcgggggccg
     8581 agcaggggct tgcgggcgaa gacgggcagg atctgcgaca cgatgccgaa gccgggcagg
     8641 atcgcgatgt agacctcggg atgcccgaag aaccagaaca gatgctgcca cagcagcggc
     8701 gagccgccgc tgtgcggctg cagcgtgtcc gaaatcacga ggccggccgg gatgaagaag
     8761 ctcgttcccg ctacgtgatc gaggatcagc aggatgcagg cgggcaacag gaccgagaac
     8821 gcgatcaggc cgatcaccgc ggtgatgaac caggcccagg tggagtacgg caggcgggcg
     8881 agcgtcatgc ctttggtgcg caggtcgagc gtcgtggcga tgaaattcag cgatccgagg
     8941 agctgtccca cgcagaagat cgcgatcgag atcgcccaca gcagctggcc cgtgccctga
     9001 cccgggccgg cgtccttgcc cacggcgctg agcggcgcgt aggcggtcca gccggaaatc
     9061 ggcggtccgg ccgggacgaa gaacgcggcc accagcacca ggaaggccag gaacgtcacc
     9121 cagaacgaca tcatgttgaa gcgggggaac gccatgtctt cggcgccgat ctggatcggc
     9181 agcgcgaaat tgccgaaccc ggcgaacggc gccgtcgtca gcacgaagaa caccatgatc
     9241 gtgccgtgca tcgtcatgag cgagaggtag aactcgggcg tcatgacgcc gccgggcgcc
     9301 ccgttgggcc acagcttgcc gatcaggggg agggcgagat cgggattggc caggtgcagc
     9361 cgcatcagcc acgacagcac catgccggtg cacaccgcga ccagaccgag accgagatac
     9421 tgctttccga tcaccttgtg gtcgaggctg aagacgtgct tgcggagaaa gctctgcggt
     9481 ggcccgtgcg cgtgcgcggc ggccggtgcg gcggtcgcgg ggtgactcat tgctgggccg
     9541 cctggtcttt gagccactgc gcgaattgtt cgggcgccat gacttccacg taggccttca
     9601 tgttgtagtg gcccaggccg cagagctgcg tgcacaccag ctcgaatttc cccgtgcggg
     9661 tcgccgtgaa atggagcggg atcacgagcc ccggcacgaa atcctgctgg atgcgcaatt
     9721 ccgggacgta gaaggcgtgc cccacatcct tggcgtgcat cgagagctgg atcggctggt
     9781 tgaccgggat ggcgagcgag cccgacacga tgtcgtcccg cgcggtggtg tcgttcgccg
     9841 ggtccaggcc gaagaagttg ccgctcgccg ggtcgatcag ctcggggtgc gtgccgccga
     9901 agactccgtc ggcgccggca tagcggaagt agaacgcgaa ctgctcggcc tggacgtcga
     9961 tgtgcagcga gccggggtcg ggcggcgcct ggtagatccc ggcccacacc ttggagccga
    10021 ccagcgtgag cgtcaggatc tcgatgccca cgaggatcga ggccagcacc accatgggcg
    10081 tggccccgcc cggaaaccgc ttgacggcgg gggcctcctt gcggtcgccg tactgccaca
    10141 cgaaccccgc cagcaccagc tgcgcgagca cgaacaggat gcccgacgca gtcatggttt
    10201 cggcgagctg atgatcgatg cggatcccgt gcagcgaata gtcgggcggc atccaccagg
    10261 tgtgccggat gaagatcgtg gtcgacacga tcgtgatgat cgcgagcagc acggcgaaca
    10321 gtttgcccac gcggtccgac ctccgctcag tacggcagcg ccttgaagac gaaattcgcg
    10381 gtggcgattc caccttcgct caccgtcacc tcctgactct gcgttccgaa ccgttcgtgc
    10441 caggccgtga cggtgtactt cccggccggc agtccggcga ggctgaactt cccgtcgttg
    10501 ccggtgaccg cgtagtgcga cgtcgtgagc acggcgaagt agccgtgcat ccaggggtgc
    10561 acgttgcact tcacggcgat gaattccggc ttgtcgtagg tcgcgtggat cggcggcgtc
    10621 ccgggcggct gggacttgtt ccactccggg ttgaccttgg cgagcgggtg aatgttgtga
    10681 gacgtctgat cgttgttgta gatgtcgagc ggctggcccg tctgcagcac cgccacgtgg
    10741 gggacgtact ggcatccctt ctggtcgtac cggacggcct gcgtgggcgc ggcgccgggt
    10801 tggccgccgg ccgagacgta caccaccacg tactccaccg tgctgccggg gccggtgacc
    10861 acgttctcgg tcatgaccgg cgtggtgtgc tgcttggcgc aggacggctc cttgctcatg
    10921 tcgatgggcc gcatcttcgg cggcgtgccg gtgaaggtca ccgtgccgct gatggtgccg
    10981 gcggcggcgg gccgcgccgc cggggcgtcg gtcgccgcgg cggcgagtcc ggcgagggcg
    11041 agcaccccga tggccggggc gaaatgtcga ttcattgcgt gcctccttcg gggccgatcg
    11101 gtttcaattc gccccgatgt gtttgcagcc agctctgcca ggtcaacgcc tccggcgatt
    11161 tgacgggctg cagcgtccgg aggaaatgca ccaggtccca ggcctcgttg ggctggaggt
    11221 tgtcgatgaa cgagggcatg ggggtgccgt ccaggccggt catgaagatg cgatacaggt
    11281 cctggttggt cgcgccgcac ttgaagcgcg tgccggacga gaaattgtac ggccggatcg
    11341 ggttgtcctt gctgtcggtc aaggtggacg ccgacggacc gtcgccgtgc ccggcggggc
    11401 cgtggcactt ccagcactcc agccgctgga aggtctgccg gccgcgcagg atgctctcga
    11461 tcgtcgcggg agtctcggtc gggatcgtga taggcgttgg ggcgggggcg ccgctccatt
    11521 tcggcgaaaa cgtcttgatg taggcgacca ggtcgacgcg gttctgcggc gtcagcgcca
    11581 cccagggggg catgttcgtc gtgacgaatc cgcgtgtcac ggcgtcgtag aggtcctggt
    11641 cggtcggcag gttgcccgac ggcgtggagc ggcacttgaa cacggcagcg gtgaagtccc
    11701 gcggcttggg atcgatccac atggcgttct ccccgttgcc gtcgccgtcc tggccgtggc
    11761 agccgacgca gtaccgctga tacagccgcg cgcccctcgc ggcctgaccc gtgacgttcg
    11821 cgatgtgcgt cgcctgcgcc acgagcggcg tcacgccgcc gaggcagagc acggcgcccc
    11881 aggccgacac gagaggaatc cagcgcgatt gcggtctcat gtggtggctc ctctctcaga
    11941 aggcgaagtc gaacgcgagc aggaaactga ggctgcggat cttcgtcgcg ggatccacgg
    12001 catcgacccc ggacccgtcg ccgctcagcg gtgccatccc ggtcgtgttc gtcagcgcga
    12061 actcgccgtg gatcgccatc ccgtcacggc tgaacaggaa gggataggcg cggaagccga
    12121 gtgcaaacgc gtcggcgttg ccgaacgact tgggcgtcgc ggggtcggcc tgcttcgaca
    12181 tgcgcagcag ctcgtagcgc ccctggacca tcaggcgggg cgtcgcgacg tagtgcgcct
    12241 cgagcagcgc cgtgttccac gcgggcttct ccgtgctgcc gcccagcgcg gcgtcgtcct
    12301 gcgcgtgggt gaccatcggg aggagctcca ggttgcccag ccagacctgc gccgtcgccc
    12361 cgattcgcac gaaggacttg ttggccgagc ctgtcccggg aatcggctcg ccgccgatcg
    12421 tctcgatggc cgtcgggcgg cggcccagat agccgaacag cccgatgcgc gtcggaccca
    12481 gcccgctgcc catcgcccac gcctggctca gcgagaccat ggcgtccatg gacttgcctc
    12541 ccggcaggcc cgcctcgccg tcgtcggtcg tgagcagcga cacgctgtag cgcgtgtagc
    12601 tgttgaccga gtggcccgac agctcggcgc cgatctgatt gtccccgagc ccgaaggcat
    12661 agctgtccac gctgccggcg ggctcatagt ggtaggcgta gaggaaaccg ccgttgttcg
    12721 acagccaggt gaaccgcttc tcggagagca ggttgtccag ctcgaacttg ccgactttca
    12781 cgttggccca cggcgagttg cccagattgt cgaagcgcac gaaggcggcc tcgactcccg
    12841 tggtgccgtc ggcgtccagc gtggggacga gcccgaacgt gacgttcttg tacagggtcc
    12901 cgaggaacag aaagtcgatg ccggagaggt cgaaccccgt ctggctgacc gtcttgcccg
    12961 cgggcaccgc gtcggtgacc tggtgggtcg tgttttcggc gtgccactgc ggcgtggtcc
    13021 ggatcgcgac cggccagtac gcgttgcttt gccagatcgg cgagtcgcgg tcgttgccga
    13081 gctggtagcc gttgtccttg aaccgttgcc cgaaagcgct cagctcgggc cacaccgtgt
    13141 ggcaggcgga gcagggcagg ccgtacttgc gcgcgaacgc cgggatggcg gcgcgggcgt
    13201 gcaacgcccg cgcgacggat cgcgtgtagg cggtgtaggt ggccgccatc tcggcggtgc
    13261 ggaacgcgat tgccgtgccg ggcgcggcgg cgggcgaccc gcttccaggg gcggcgccca
    13321 ccgtgcagag caggattgca gcgacgaggc ccttcgtgag ccgctcgacc ataccgcctc
    13381 ctgtgtagtg cggtacgcag tggggagtga cacgcctttc gatggctttc tccgcgagaa
    13441 cctcctcaag taactgccaa ttatcactct gtccggttcc tgtcaaggtt ccgagcgagg
    13501 ggtgggatct ggaggggtca gttcgccttg agcacgtcct ccacgaagct cagcgccttg
    13561 gtcgcgaccc actcggccga cacggcgtcc accccgatcg ttcccgtgcg gtcaccggtc
    13621 gccggcgcga cccggccctt ggcgggcgac agcttgacgt cgcgctgcac cacgatgccg
    13681 cggcgcggat cgtacttgaa catcagggcc gacgcgagca cggcctcggg actcttcgcg
    13741 gcggcaacgc tccggggagt gaaggacagc gccacactgg ggtaggcgtc gtcgcgatcg
    13801 gtgtgatccc gggtggaggc gtcgtgcccg gccttcttga gctcctccat gaaccgctcc
    13861 agcaccggtc ggacgaccgc gcgcaggagg tccgcacagg cgcgccgcct gcgctcggcg
    13921 gtgctgtccc cggccctgga agccggcttc gcggccgcct ggcgttccgc ataggccgag
    13981 agcagggcgt ggagcttcgc gatgcgctcg tcggtcatga aggggaagta taagaggtca
    14041 ggtcggagag acgaggggca gatagcgatc gcgcagcacg cgcaggtgat gcccttcgtg
    14101 gcccacgatg tgaaaggcca ggccgcgaac cgtcacgtcg tgtccctcca gggttccacg
    14161 gcgggtccac gcctcgggcc cgagcccggc gaacagcgtg atcgaggccg tccgcacgct
    14221 gcggtattcc tccagcaagt cgggcaacga gcgggcttcg ctcccggcag cgtcggcgta
    14281 gcggacctga tcgaaccctg ccagcacacc atcgtctccg cgagccagac ggagcgcccg
    14341 gtaggcgtag atccgctcgt cgtcgctgag atggcccaac agatccttga tcgtccattt
    14401 atccggtgcg tagcgcaccc cggtcactcg cgcatcggtg agcgcggaga acagagccat
    14461 cacccggtcc cgcgaggatc gcagggccgc gaccgcgtcg tcctcgggga tcgcggcgat
    14521 ctcctcgtgg gcgtaggcgg cgtattcccc cggctggggg cggccggacc ggctgcgcgc
    14581 ctcgggctcg agggcgcgaa attcccttgc gctcattaga atcaggatgc gcgacagaag
    14641 gcgcagaatc aacctacccc gaaatggaga gcgtcatgaa gctggatgca cgcacggtcg
    14701 ccctggtcct gatgggagct cccggattgc tcgccgccca gagcgcagcg agcccggtgg
    14761 cggacgcatt tcgcacctat gaagcgcgct cggcccggaa gctcgtcgcc gccatcgagg
    14821 cgatgcccgc cgacaagctc ggctacaagc ccacaccggc gcagatgagc gtcgccgcca
    14881 tcgcgttgca cctcgcggag ggcaacgact acttctgctc caccatcagc gggacgccgg
    14941 ctccgaagcg ggactcggtc accgcctcgt cgtccaagga ggcgctcgtg gcacggctgc
    15001 gcgagacgtt cgacttctgc cagacgacac t
//
LOCUS       DBNGZW010000160         6978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_901202, whole genome shotgun sequence.
ACCESSION   DBNGZW010000160 DBNGZW010000000
VERSION     DBNGZW010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6978
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_901202"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="AC16JC_03965"
     CDS             complement(<1..940)
                     /locus_tag="AC16JC_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR5163885.1"
                     /translation="MARTTPLDRIRNIGIMAHIDAGKTTTTERILYYTGRTYKLGEVH
                     EGTATMDWMEQEQERGITITSAATTTFWPRRGEQFRINIIDTPGHVDFTVEVERSLRV
                     LDGAVTVLDAVGGVEPQTETVWRQADRYGVPRIVFVNKMDRVGSDFDRCLKMVRERLG
                     AKAIPIQLPLGSGELFTGVIDLIREIEIVYDDETLGKKYVEGPVPAPLKDRVAQMRHE
                     MLEAVVETDGAILRKYLDGQALSEEEIRTVLRKATIGGHVVPVICGAAFKNKGVQQLL
                     DAVVDYLPAPVDVPPIQGHLPHHDATHVERPASDEAP"
     gene            complement(1067..1537)
                     /gene="rpsG"
                     /locus_tag="AC16JC_03970"
     CDS             complement(1067..1537)
                     /gene="rpsG"
                     /locus_tag="AC16JC_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HIR5163886.1"
                     /translation="MSRRKKAVKRPVPADPRYDSQTVSKFVNNLMERGKKSLAERVFY
                     SAMDLIEQRTGQPGVNVFKQALSNVKPALEVKSRRVGGATYQVPVEVRPERRTALGMR
                     WIIQYSRSRKEKDMSERLAAELILASKGEGESIKKKEDTHRMAEANRAFAHYRW"
     gene            complement(1534..1917)
                     /gene="rpsL"
                     /locus_tag="AC16JC_03975"
     CDS             complement(1534..1917)
                     /gene="rpsL"
                     /locus_tag="AC16JC_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006632197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HIR5163887.1"
                     /translation="MPTINQLIRHGRRQVAKKEKAPALRGNPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGFEVTAYIPGEGHNLQEHSIVMIRGGRVKDLPGVRYHIIRGTL
                     DAAGVNERKQSRSKYGAKRPKEAGK"
     gene            2076..2450
                     /locus_tag="AC16JC_03980"
     CDS             2076..2450
                     /locus_tag="AC16JC_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5163888.1"
                     /translation="MAAKTILLAEDDRVTREALAAQLRGAGFQVLTAVDAMQAGMIAQ
                     RSAPDAMVLDIQIPGGTGLETLKRMRASSRTVLIPVIAISGSATPEQVERARDLGAAD
                     FFPKPVDFERLREVLGRLLADP"
     gene            2584..4812
                     /locus_tag="AC16JC_03985"
     CDS             2584..4812
                     /locus_tag="AC16JC_03985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HIR5163889.1"
                     /translation="MATRIRPMPSLTPLLLAVLVPLSACTPGGPARPTTVPTARAGAD
                     TTKKDVPWKPWAEVTKDTRQLTGLFTAYVKRDNVYLDLKPGQFDKDFLLVTQLSRGLG
                     DFGFDGGTDMRSDLIRFHRTPTKVELVLVNPYLTASPGTPMARTVDYSFGASVAYAFP
                     IASIRDTTDEVLIDLAPFLVSDFADIGAFFSFVVQALRLPGGISLDRERSAFQDISLF
                     PTNLEADARLSFLASRPIGISTVPDYRYLPMTVHYSLVELPATPMRPRYADDRVGYFI
                     AAMMNFSRDTSESFFVRYVNRWRLEKKDPAAAVSDPVKPITYYLDRTIPEEWRPYVRA
                     GMLEWNKAFEAIGYRNAIQVLDAPDDTLWSAEDARYSTVRWMANFGATYAIGPSDVDP
                     RTGEILNADILIAASWIQAWQGEYHEYSGPEAMTRQVFAEDSLLRADPQGRLARACTY
                     AGGLTRYGTILRATLAANGAIPPGAPVPREYIGQALKELVMHEFGHTLGLRHNFRGTA
                     AIPAAELGNRSYTATHGTSASVMDYNPPAIAMDRSKQGDYYSTTIGTYDRWAIRYGYA
                     DMGGATPDAERPGLARVAGEAADPDHVYATDEDAGFAGFGADPNVTRYDQTADPLGWA
                     KDQVTLVNRLFDSLELRLVAPGDGYPRLRSGFLDLLFQRWYATLVTTKYLAGVYTARD
                     HRGDPQGRAPFTAVPAAKQRDALTFIASAGLGENSYRFRPELLNKLAPSRWWHWGQNP
                     FS"
     gene            4867..5286
                     /locus_tag="AC16JC_03990"
     CDS             4867..5286
                     /locus_tag="AC16JC_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163890.1"
                     /translation="MDLLLDPGVLARVRDNELRAPPGTAVVTIPDIFATMTNAIWAEA
                     GMGGSRPRNAGSIRRDLQRMYLTELTRMVVNPALGTPDDARTIARATLADLATRLSHA
                     LNAGGLDTYTRAHYADSRQRIAQALNAQVVVPAAQLR"
     gene            complement(5366..>6978)
                     /locus_tag="AC16JC_03995"
     CDS             complement(5366..>6978)
                     /locus_tag="AC16JC_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR5163891.1"
                     /translation="GSKSAGAAKPAGAARDTAAKTPAPVAIELDGFEQRAVALPPAAG
                     NYADLQAVSGKVVYRRPPRTGSSDQKTPLMTYDLKDRKEQTVIDDANAALVSADGKKV
                     LIRQARRWAILDLKPDQKLETPLRTAELAMLVDPPAEWKQIFADVWRFERDYFYDPNM
                     HGVAWSAMRTQYGLLLDGAVTRWDVNFVIGELISELNASHTYRGGGDLEAAPNRGVGL
                     LGVDWSLENGAYRIKRIIDGAPWDGEVRSPLTAPGVNVKAGDYVLAVNGMPLDTSQDP
                     WAAFDGLAGKDVRLTLNSRPSREGARDVVVTTLENESRLRYLAWVEANRLRVDEATQG
                     KVGYVYVPSTGLDGQTELLRQYLAQVGKQGLIVDERWNSGGQIPDRFIELLDRPALAY
                     YAVRDGRDWQWPQNAIIGPKVMLINGWSGSGGDAFPTFFREAKLGPLIGTRTWGGIIG
                     ISGVPTLIDDGNVTVPTFRQYDPQGKWFAEGHGVEPDIEVVDDQAQLAKGVDPQLERA
                     IQEVMKAVQAAQPLAPKRPAYEKRVAGN"
BASE COUNT         1035 a         2620 c         2151 g         1172 t
ORIGIN      
        1 acggcgcctc gtcggaggcg ggacgctcga cgtgcgtggc atcgtgatgc ggcaggtgcc
       61 cctggatcgg cggcacgtcc accggcgcgg gcaggtagtc gaccaccgcg tcgagcagct
      121 gctgcacgcc cttgttcttg aacgccgccc cgcagatcac cgggaccacg tgcccgccga
      181 tcgtcgcctt gcgcagcacg gtgcggatct cctcctccga cagggcctgc ccgtcgaggt
      241 acttgcggag gatcgcgccg tcggtctcca ccacggcttc gagcatctcg tgccgcatct
      301 gggcgaccct gtccttcagc ggcgcgggca ccggcccttc cacgtatttc ttgcccagcg
      361 tctcgtcgtc gtagacgatc tcgatctccc ggatcaggtc gatgacgccg gtgaacagct
      421 ccccggaccc cagcggcagc tggatcggga tcgccttcgc gccgagccgc tcgcgcacca
      481 tcttgaggca gcggtcgaag tcggagccca cgcggtccat cttgttgacg aacacgatgc
      541 gcggcacgcc gtaccggtcc gcctggcgcc acaccgtctc ggtctgcggc tccaccccgc
      601 ccaccgcatc gagcaccgtg acggcgccgt ccagcacccg caggctccgc tccacctcga
      661 ccgtgaaatc cacgtggccc ggcgtgtcga tgatgttgat gcggaactgc tcgccccgcc
      721 gcggccagaa cgtcgtggtg gcggcggagg tgatggtgat cccccgctcc tgctcctgct
      781 ccatccagtc catcgtggcc gtaccttcgt gcacctcacc cagcttgtag gtgcggcccg
      841 tgtaatagag gatgcgttcg gtcgtagtcg tcttcccggc atcgatgtgg gccatgatgc
      901 cgatgttgcg aatgcgatcg agcggtgtcg tgcgtgccat tgccgtcctt tatacagcaa
      961 aaacgcgggg cgaccgcgac gcccaaccct gggccggtct cgctccgcca tcatcgggag
     1021 gcccgagggc cccctcgacg cggtccgttg tgctgctgtc cctagcctac caccggtaat
     1081 gcgcgaacgc cctgttggcc tccgccatcc ggtgcgtgtc ttccttcttc ttgatcgact
     1141 cgccctcgcc cttcgaggcg agaatcagct ccgccgcgag ccgctccgac atgtccttct
     1201 ccttgcggga gcggctgtac tggatgatcc agcgcatccc cagcgcggtg cggcgctccg
     1261 gccggacttc caccggcacc tgataggtgg cgccgcccac gcggcgcgac ttcacttcca
     1321 gcgccggctt cacgttcgac agcgcctgct tgaacacgtt cacgcccggc tgcccggtgc
     1381 gctgctcgat caggtccatc gccgagtaga acacccgctc cgccagcgac ttcttgcccc
     1441 gctccatcag gttgttcacg aacttggaga cggtctggct gtcgtaacgc ggatcggccg
     1501 ggaccggccg cttcaccgct ttcttgcgcc gactcacttc ccggcctcct tgggccgctt
     1561 ggcaccgtac ttcgagcgcg actgcttgcg ctcgttcacc ccggccgcgt ccagcgtgcc
     1621 gcggatgatg tggtaacgca cccccggcag atccttcacg cggccacccc ggatcatcac
     1681 gatcgagtgc tcctgcaggt tgtgcccctc gcccgggata taggccgtca cctcgaaccc
     1741 gttggtcagc cgtacgcggg cgactttccg cagcgccgaa ttcggcttct tgggcgtcgt
     1801 cgtgtagacg cgcgtgcaca cgccgcgctt ctgcgggttg ccgcgcagcg ccggcgcctt
     1861 ctccttcttt gcgacctgcc ggcgcccgtg ccggatcaac tgattgatgg tcggcatacc
     1921 ccttgtgccc agcccccgaa aaaagttccg aaacagactg taaagctagc cggacgcttg
     1981 gagttaggtc aacccccacg agcgccaacc gccgcgggtc gcaccccgca acgggtgatt
     2041 acggacttgg cgcccacgcg ccagaattag ggccgatggc cgccaagacc attctgctgg
     2101 ccgaagacga ccgggtcacc cgggaagccc tcgcggccca gctgcgaggc gccgggtttc
     2161 aggtgctcac ggcggtggac gcgatgcagg ccggcatgat cgcgcagcgc agcgcgccgg
     2221 acgccatggt gctggacatc cagatccccg gcggaacagg gctcgaaacc ctgaaacgca
     2281 tgcgggcctc gtcaagaacg gtgctcatcc ccgtgatcgc gatcagcggg tcggcgacac
     2341 cggagcaggt ggagcgggca cgcgacctgg gcgccgccga tttcttcccc aagccggtcg
     2401 atttcgagcg tctgcgcgag gtgctgggcc gcctcctggc cgacccctga gaggtcctgg
     2461 agccttcccc aagcccccat ctgtccgcgt cacgcgagtt acgtaagcct gacgatcccc
     2521 tctcttaccg accccgggtg cgtcccctag aatcaggatt tccactcctc cggagccccg
     2581 cccatggcta ctcgcatccg accgatgccg tcgctcactc ccctgctgct tgccgttctc
     2641 gtgcccctct ccgcctgcac cccaggcggg ccggccagac ccacgaccgt ccccaccgca
     2701 cgcgccggag ccgacaccac caagaaagac gtcccctgga agccctgggc cgaggtcacc
     2761 aaggacaccc ggcagctgac cggcctgttc acggcctacg tgaagcggga caacgtctat
     2821 ctcgacctca agccgggcca gttcgacaag gatttcctcc tcgtgaccca gctgtcgcgg
     2881 gggctggggg acttcggatt cgacggcggc accgacatgc gctcggatct gatccgcttc
     2941 caccgcacac ccaccaaggt cgagctggtg ctcgtcaacc cctacctgac ggccagcccg
     3001 ggcaccccca tggcccggac cgtggactat tcgttcggcg cctcggtggc ctacgcgttt
     3061 ccgatcgcca gtatccggga tacgaccgat gaggtcctga tcgacctggc tccgttcctc
     3121 gtctccgact tcgccgatat cggcgccttc ttcagcttcg tcgtgcaggc cttgcgcctg
     3181 ccgggcggca tctcgctcga ccgggaacgc tcggccttcc aggacatcag cctcttcccc
     3241 accaacctcg aggccgacgc acgcctgagc ttcctcgcct cgcggccgat cggcatcagc
     3301 acggtccccg actaccgcta cctcccgatg accgtgcact actcgctggt cgagctgccg
     3361 gccacgccga tgcggccgcg ctacgccgac gaccgcgtcg ggtacttcat cgcggcgatg
     3421 atgaacttct cgcgcgatac gtccgaatcg ttcttcgtgc gctacgtcaa ccgctggcgc
     3481 ctggagaaga aggacccggc ggcggccgtg agcgaccccg tcaagccgat cacgtactac
     3541 ctcgaccgca cgatccccga ggagtggcgc ccctacgtcc gcgccgggat gctcgagtgg
     3601 aacaaggcgt tcgaggcgat cggctaccgc aacgccatcc aggtgctcga cgcccccgac
     3661 gatacgttgt ggagcgccga ggacgcgcgt tacagcaccg tccggtggat ggccaacttc
     3721 ggcgcgacct acgccatcgg tccttccgac gtggacccgc gcaccggcga gatcctgaac
     3781 gccgatatcc tcatcgcggc ctcgtggatc caggcgtggc agggggaata ccacgagtac
     3841 tcaggacccg aggccatgac ccggcaggtc ttcgccgaag actcgctgct ccgcgccgac
     3901 ccgcagggcc ggctggctcg ggcttgcacg tacgccggcg gtctcacccg ctacggcacc
     3961 attctgcgcg ccaccctggc ggcgaacggc gcgatcccgc ccggagcgcc ggtgccgcgg
     4021 gagtacatcg ggcaggcgct gaaagagctg gtcatgcacg agttcgggca tacgctcggg
     4081 ctgcggcaca acttccgtgg caccgctgcg attccggccg ccgagctggg caaccgatcc
     4141 tacacggcga cgcacggcac ctcggcgtcg gtgatggact acaacccgcc cgccatcgcg
     4201 atggaccgca gcaagcaggg cgactactac tccaccacca tcggcaccta cgatcgctgg
     4261 gcgatccgct acggctacgc cgacatgggc ggcgccacac cggacgccga gcgtcccggc
     4321 ctggcgcgcg tcgccggtga ggcggccgat cccgaccacg tgtatgcgac cgacgaggac
     4381 gccggcttcg cgggattcgg cgccgatccc aacgtcacgc gctacgacca gaccgccgat
     4441 cccctcggct gggccaagga ccaggtgacg ctggtcaacc ggctgttcga ctcgctcgag
     4501 ctccggctgg tggcacccgg cgacggctat ccccgtctgc gctccggctt cctcgacctg
     4561 ctgttccagc gctggtacgc gaccctcgtc accacgaagt acctggcggg cgtctacacc
     4621 gcccgcgacc accgcgggga tccccagggc cgcgcgccgt tcaccgccgt tccggcagcg
     4681 aagcagcgcg acgcgctcac gttcattgcc agcgccggtc tgggggaaaa cagctatcgg
     4741 ttccggcccg agctgctgaa caagctcgcc ccgtcgcgct ggtggcactg gggccagaac
     4801 ccgttctcct aggggcgcat cgatttcccg ctgcacgact gggcgctcgc gttccagggc
     4861 accctggtgg atctgctgct cgatcccgga gtcctggccc gggtccgcga caacgagctg
     4921 cgggcgccgc cgggcacggc cgtggtgacg attcccgaca tcttcgccac catgaccaac
     4981 gccatctggg ccgaggcggg gatgggcggg agccgtccgc gcaatgcagg atcgatccgg
     5041 cgcgacctgc agcggatgta cctgacggag ctgacgcgca tggtggtgaa tccggccctc
     5101 gggacacccg acgacgcgcg taccatcgcg cgggcgaccc tcgccgacct ggcgacccgg
     5161 ctctcgcacg cgctcaacgc gggcggactc gatacctaca cccgggcgca ctacgccgac
     5221 tcacgccaac ggatcgcgca ggcgctcaac gcccaggtgg tggtgccagc cgcgcagctt
     5281 cgctgacgca gaaggttggc ggaggatggt ggaagacggt gaagggcggt cgaggtcaac
     5341 ctccaccgtc ctcccccgtc tttctttagt ttcccgccac ccgcttctcg tacgccggcc
     5401 gcttcggcgc cagcggctgc gccgcctgca cggctttcat tacttcctgg atcgcgcgct
     5461 cgagctgggg atccaccccc ttcgcgagct gcgcctggtc gtccaccacc tcgatgtccg
     5521 gctccactcc gtgcccctcg gcgaaccact tcccctgcgg gtcatactgg cggaacgtcg
     5581 gcacggtcac gttgccgtcg tcgatcagcg tcggcacgcc gctgatgccg atgatcccgc
     5641 cccaggtgcg cgtgccgatc agcggcccga gcttcgcctc gcggaagaac gtcgggaacg
     5701 catcgccccc tgagccgctc cacccgttga tcagcatgac cttcggcccg atgatggcgt
     5761 tttgcggcca ctgccaatcc cgcccgtcgc gcaccgcgta ataggccagc gccggccggt
     5821 cgagcagctc gataaaacgg tcggggatct gtccgccgga gttccagcgc tcgtccacga
     5881 tcagcccctg cttgcccacc tgggccagat actggcgcag cagctcggtc tggccgtcga
     5941 gcccggtact cggcacgtac acgtagccca ccttgccctg cgtcgcctcg tccacccgaa
     6001 ggcggttcgc ctcgacccac gccaggtagc gcagccggct ttcgttctcg agcgtggtga
     6061 ccaccacgtc ccgcgcgcct tcccgcgacg gccggctgtt cagcgtgagt cgcacgtcct
     6121 tccccgcgag cccgtcgaac gccgcccacg gatcctgcga cgtgtcgagc ggcatgccgt
     6181 tcaccgccag gacgtaatcg ccggctttca cgttgacgcc cggcgcggtg agcggtgagc
     6241 ggacttcgcc gtcccacggc gcgccgtcga tgatccgctt gatccggtag gcgccgtttt
     6301 ccagcgacca gtccaccccc agcagcccca ccccacggtt gggggccgct tccagatcgc
     6361 cgccgccccg gtaggtgtgg gacgcgttca gctcggagat cagctcgccg atcacgaagt
     6421 tcacgtccca gcgcgtgacc gcgccgtcga gcaggagccc atactgggtc cgcatcgcgc
     6481 tccaggccac gccgtgcatg ttggggtcgt agaagtagtc ccgctcgaag cgccacacat
     6541 cggcgaagat ctgcttccac tccgccggcg gatcgaccag catcgcgagc tccgccgtgc
     6601 gcagcggcgt ctcgagcttc tggtccggct tgaggtcgag aatcgcccag cgccgcgcct
     6661 gccggatcag caccttcttg ccgtcggcgg acacgagcgc cgcgttggca tcgtcgatga
     6721 ccgtctgctc cttgcgatcc ttcaggtcat aggtcatgag cggcgtcttc tgatccgacg
     6781 acccggtccg cggcggccga cggtacacga ccttgccgga caccgcctgc agatcggcgt
     6841 agttcccggc cgccggcggc agggccaccg cccgttgctc gaacccgtcc agctcgatcg
     6901 ccaccggcgc cggggtcttt gccgccgtgt cccgcgccgc ccccgccggt ttcgccgcac
     6961 ccgcgctttt gctccccg
//
LOCUS       DBNGZW010000161         3733 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_970702, whole genome shotgun sequence.
ACCESSION   DBNGZW010000161 DBNGZW010000000
VERSION     DBNGZW010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3733)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_970702"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="AC16JC_04000"
     CDS             complement(<1..320)
                     /locus_tag="AC16JC_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163892.1"
                     /translation="MRRNGIVLLAVVATVATTSPAYGGGTGTRLSNYMASYSAHASIP
                     AWARKYNMNCSGCHYPAAPRLNATGIRFRWAGYRMPEEMGEKVDVSQVSNYLAVRGRM
                     RYEY"
     gene            complement(573..2024)
                     /locus_tag="AC16JC_04005"
     CDS             complement(573..2024)
                     /locus_tag="AC16JC_04005"
                     /inference="COORDINATES: protein motif:HMM:NF025016.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HIR5163893.1"
                     /translation="MPSPFRWLDERLDLRRAKRDLLDREVPARLTWWHTLGSATFTVF
                     LVQVITGTTLATYYSASPDHAYDSIQYISHQVASGTLLRGIHHWGASLMVVLIVAHMI
                     RVFAVGAYKYPREINWLLGVGLFLIVLGFSFTGYLLPWDQKAYWATAVGTNIAGTTPF
                     IGGALVALLRGGSELGAATLARFYAFHSLWLPLALTVVVALHLIVLIRQGIAARPRDL
                     EVGAPARTSDPKYPEYYREAYAATKRGGMRFWPDILGKDAIVSAAVVLLIVLMAVVLR
                     TPLELPADPTDTAYIPRPEWYFLPIYQLLKLVPGSMESLIAVGVPGVLVLVLLGLPFF
                     DRGSTRNLLHRPAALVSLALILGGAGFLIGAAGRGPRAQEEGVALTSLQRAGRALYQS
                     QQCAGCHRVAGQGGEEGPDLTTVGLRHSSGWLHSFLEAPSRFHPESRMPAFAPPALTH
                     QEIEELSQYLSSLRGRENVPPQYYDTFPPPRQP"
     gene            complement(2029..2559)
                     /locus_tag="AC16JC_04010"
     CDS             complement(2029..2559)
                     /locus_tag="AC16JC_04010"
                     /EC_number="7.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="QcrA and Rieske domain-containing protein"
                     /protein_id="HIR5163894.1"
                     /translation="MATGPQQTPPAGRRTFLRWATAVGGFFSVVLAGVPAVRAFFSPT
                     FKRAQPEKWIPLGPADQFDPETPTKVDFVDTGADAWIETRRLRSVWIYTEDGEHFTVY
                     NGRCTHLGCNYALDQTANVFRCPCHTGYFEIKTGKVLGGPPPRQLDQLPVKVENGVVY
                     AAYEDFRLGVPEKVRA"
     gene            complement(2587..>3733)
                     /locus_tag="AC16JC_04015"
     CDS             complement(2587..>3733)
                     /locus_tag="AC16JC_04015"
                     /inference="COORDINATES: protein motif:HMM:NF021235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5163895.1"
                     /translation="MTRETPQGTQTLSCQDCHIAPGGQWMAVRRATPEGCLACHGPGV
                     TQHLAAPDSLCQTCHLPLVRAATLQPADIAGFPAPPSHQAPNFVSRAGHGALAKGTTE
                     PVAASCTVCHAREFCVTCHVDAPEQPAIQALGADARSQAIAVRLAAPASHRASDFLSR
                     HGGMLREDARACATCHTRESCLACHAPSQRVSAALHSSGDGRGRGAQPVRHPPESHRE
                     NFAVRHPQAAATTPASCAGCHVRTDCLECHRPAAGSRPGYHPAGFLTLHPAAAYARQT
                     SCSDCHNVGSFCATCHAAAGLVSSGPLRSGYHDASRFFVAGHGQAARQSLESCTACHV
                     ERDCLTCHSATGGRRFNPHGPGFDANRLRRKNPEMCIACHGTDIPTR"
BASE COUNT          611 a         1256 c         1294 g          572 t
ORIGIN      
        1 ctgtactcgt agcgcatgcg gccacgcacc gcgaggtaat tcgatacctg gctgacgtcg
       61 accttctcgc ccatttcctc gggcatgcgg tagccggccc accggaacct gatgcccgtg
      121 gcgttcagcc ggggcgccgc ggggtagtgg cagccggagc agttcatgtt gtacttgcgg
      181 gcccacgcgg ggatgctcgc atgagcgctg taggacgcca tgtagttact gagccgcgtg
      241 ccggtgccgc cgccgtaagc gggactggtt gtggccaccg tggccacgac ggcaagcagt
      301 acaatgccgt tcctccgcat tagaccctcc ctctcgtgaa gtgcgcatcg aagccggaca
      361 gctctggtcc aacgcgggaa tacttcctgc tttttcgtga aggaatcatg agatacgcct
      421 gaggactatt caggcaggaa tcacggttct ttccttatct cgaccccgca catggatgcc
      481 atcgtgaacg ccgtccaaga aagggccccg cgagcagtcg cggggcccct ttcaatccgg
      541 cgtacgcgag gcaagcgccg atctgtcgcc gctcagggct gacgcggagg gggaaacgtg
      601 tcgtagtact gcggcggcac gttttcccgc ccccgcaacg agctgagata ctgcgacagc
      661 tcctcgatct cctggtgcgt gagcgcgggc ggcgcaaacg ccggcatgcg cgattccggg
      721 tggaaccggc tcggtgcttc gaggaagctg tgcaaccacc cggatgagtg gcggagcccg
      781 acggtcgtga gatccgggcc ctcctctccg ccctggcccg ccacgcggtg gcagcctgcg
      841 cactgctgcg actggtacag ggcacgcccc gcgcgctgca gcgaggtgag cgccacgcct
      901 tcctcctggg cacggggacc gcggcccgcc gcaccgatca ggaatccggc tcccccgagg
      961 atcagcgcca gcgaaacgag cgcggccggc cggtgcagca gattgcgcgt ggacccccgg
     1021 tcaaagaacg ggagccccag caggacgagt acgagtacgc ccgggacgcc caccgcaatc
     1081 aggctctcca tggaccccgg aaccagcttg agcagctgat agatcgggag gaagtaccat
     1141 tcgggtctcg gtatgtacgc ggtatcggtc ggatccgcgg gaagctccag cggcgtccgc
     1201 aatacgaccg ccatcagcac gatcaggagg acgaccgccg ccgacacgat ggcgtccttc
     1261 cccaggatgt ccggccagaa tctcatcccc ccgcgcttgg tggcggcgta ggcctcccgg
     1321 tagtactccg ggtacttggg gtcgctcgtg cgcgccgggg cgccgacctc caggtccctc
     1381 gggcgcgccg cgatgccctg gcggatcagg acgatcagat ggagggccac cacgaccgtg
     1441 agcgccagcg gcagccagag cgagtggaac gcgtagaagc gggccagcgt cgcggccccc
     1501 agctccgatc ctccgcgcaa cagcgcgacc agcgctccac cgatgaacgg cgtcgtcccc
     1561 gcgatgttgg taccgaccgc cgtcgcccag taggccttct ggtcccaggg cagcaagtag
     1621 cccgtgaaac tgaagcccag cacgatcagg aacagcccca cgccgagcag ccagttgatc
     1681 tcgcgcgggt acttgtacgc ccccaccgcg aacacacgga tcatgtgcgc cacgatgagc
     1741 acgaccatga ggctcgcgcc ccagtgatgg atgccacgga gcagggtgcc gctcgctacc
     1801 tggtgcgaga tgtactggat gctgtcgtag gcgtgatcgg gagacgccga gtaataggtc
     1861 gccagcgtcg tgccggtgat gacctgcacg agaaacacgg tgaacgtcgc gctccccagc
     1921 gtgtgccacc aggtcagccg ggcgggcacc tcgcggtcca ggaggtcgcg cttcgcccgt
     1981 cgcagatcga gccgttcgtc gagccaccgg aagggggagg gcatgggatc acgccctgac
     2041 tttctcggga acgcccaagc ggaagtcctc gtacgccgcg tacacgactc cgttctcgac
     2101 cttcacgggc agctggtcga gctgccgcgg cggtggaccg cccagcacct tcccggtctt
     2161 gatctcgaag tacccggtgt gacaggggca ccgaaacacg ttcgccgtct ggtcgagagc
     2221 gtagttgcac ccgagatgag tgcagcggcc gttatagacg gtgaagtgct ccccgtcctc
     2281 ggtatagatc cagacgctcc gcagccgccg cgtttcgatc caagcgtcgg ccccggtgtc
     2341 gacgaaatcg actttggtcg gtgtctccgg atcgaactga tcggccgggc ccagcgggat
     2401 ccacttctcc ggctgggccc gcttgaacgt gggagagaag aacgcgcgca cggccgggac
     2461 cccggcgagc accacggaga agaagccgcc gaccgccgtg gcccatctga gaaacgtgcg
     2521 ccgtccggcg ggtggtgtct gctgcggccc agtcgccatg ccctcacctc ctggtggtac
     2581 gacgcctcaa cgagtcggga tatccgtccc gtgacacgcg atgcacatct ccggattctt
     2641 gcgccgcagc cggttcgcgt cgaaccccgg accgtgcgga ttgaagcgtc gcccgccggt
     2701 ggccgaatgg caagtcaggc agtcgcgctc gacgtggcag gcggtgcagc tctcgagact
     2761 ctggcgtgcg gcctgcccgt ggcccgccac gaagaaacgg ctggcatcgt gatagccgct
     2821 ccgcaggggg ccactgctga cgaggccggc ggcggcgtga cacgtcgcac agaaactgcc
     2881 gacattgtgg cagtcgctgc aagaggtctg gcgcgcgtag gccgcagcgg ggtggagcgt
     2941 cagaaacccc gcggggtggt agccgggccg cgaccccgcg gcgggtcggt ggcactcgag
     3001 gcagtccgtg cggacgtggc accccgcgca ggatgccggc gtcgtggcgg ccgcctgagg
     3061 gtgccgcacg gcgaaattct cgcgatgcga ctcgggtggg tggcgtaccg gctgggcgcc
     3121 tcggcctcgc ccgtccccgc tcgaatggag tgccgccgag acccgttgcg atggggcgtg
     3181 acaggcgagg cagctttcac gcgtgtggca cgtcgcgcac gcgcgggcgt cttcccgcag
     3241 catcccgccg tgccgcgaca ggaagtctga tgcgcgatgc gaggccgggg cggcgaggcg
     3301 caccgcgatc gcctgcgacc gcgcatcggc gccgagcgcc tggatggccg gctgttccgg
     3361 cgcatcgacg tggcaggtga cgcagaactc ccgggcgtgg cagaccgtgc agctcgccgc
     3421 caccggctcg gtggttccct tggccagggc gccgtggccc gcgcggctca cgaagttcgg
     3481 cgcctgatgc gaaggcggcg cggggaatcc ggcgatatcc gcgggctgca gtgtagcggc
     3541 gcgcaccagc ggcagatggc acgtctggca caggctgtcg ggagccgcga ggtgttgcgt
     3601 cacgccgggt ccgtggcacg ccaggcatcc ctcgggagtg gcgcgacgca ccgccatcca
     3661 ctgacctccg ggggcgatgt ggcagtcctg gcacgacagg gtctgcgttc cctgcggcgt
     3721 ttcccgggtc ata
//
LOCUS       DBNGZW010000162         4858 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1714554, whole genome shotgun sequence.
ACCESSION   DBNGZW010000162 DBNGZW010000000
VERSION     DBNGZW010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4858)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4858)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4858
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1714554"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="AC16JC_04020"
     CDS             <1..285
                     /locus_tag="AC16JC_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HIR5163896.1"
                     /translation="GRGEDLNQALERAGRVADFMEFAELMCEDALQREESCGGHFREE
                     YQTPDGEAKRDDSRFSYVAAWEWKGVGTAPRPHKEPLAFEHVHLAQRSYK"
     gene            285..1061
                     /locus_tag="AC16JC_04025"
     CDS             285..1061
                     /locus_tag="AC16JC_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HIR5163897.1"
                     /translation="MRLVLHVWRQAGPEAPGKFVRYEATDVSPDMSFLEMLDVLNETL
                     VARKEEPVAFDHDCREGICGSCGLVINGRPHGPQLATTACQLHMRHFTDGEEVWVEPW
                     RARAFPVLKDLVVDRGAFDRIIQAGGFITARTGSAPEANAVPVPKRDADRAMDAAACI
                     GCGACVAACPNASAMLFVAAKVSHLGSLPQGQPERYERVVRMVATMDEAGFGACTNHG
                     ECMAACPKEISVDWIAQLNRDLMHATLRPPRAGKDWSGAA"
     gene            complement(1065..1994)
                     /gene="mdh"
                     /locus_tag="AC16JC_04030"
     CDS             complement(1065..1994)
                     /gene="mdh"
                     /locus_tag="AC16JC_04030"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR5163898.1"
                     /translation="MFQKITVVGAGNVGATAAQRVAEKNLARTVVLVDVIEGVPQGKG
                     LDQWESAPIEGFDTRVVGTNTYEPAEGSELFIVTAGIARKPGMSRDDLVRTNVEIVKQ
                     VAQQIKQVAPRAIVVMVSNPLDVMCWVAKEVTGFPRERVVGMAGVLDTARYRSFLAEA
                     LGVSVEDIQAMVLGGHGDTMVPLVSYTTVSGIPVSQLLDQATLEAIVTRTRNGGAEIV
                     NFLKTGSAYYAPSAAAVQMAEAIVLDKKRVLPCAAWLEGEYGLSGIYCGVPCKLGKGG
                     VEQILEVKLSDAERAALQRSANAVKETMAVVKL"
     gene            complement(2040..3071)
                     /gene="moaA"
                     /locus_tag="AC16JC_04035"
     CDS             complement(2040..3071)
                     /gene="moaA"
                     /locus_tag="AC16JC_04035"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013018538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HIR5163899.1"
                     /translation="MTTAPLDTLGRPLGSLRVSVTDRCNMRCSYCMPEAEYVWLPRDQ
                     ILTFEEIARLVRIFAGLGATKVRLTGGEPLLRHDLPALVRMIADEPRVRDLALTTNGV
                     LLARHAPELRAAGLRRVTVSLDTLRPDRMERFARSARHADVLAGIGAAQALGFARLKL
                     NAVVLRGFNDDELTDLIEFGRARNAEVRFIEYMDVGGATRWSLDRVVPQREILDRLAH
                     HYGAITPITGDDPRAPAEQFALPDGTTFGIIASTTAPFCRACDRSRLTADGQWLLCLY
                     AERGVDLRAPLRDGATDAEITALVRTTWLGRRDRGAEERLAVAERQPLYQVTGLRADP
                     HREMHTRGG"
     gene            complement(3068..3511)
                     /locus_tag="AC16JC_04040"
     CDS             complement(3068..3511)
                     /locus_tag="AC16JC_04040"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014448.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030366 - molybdopterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIR5163900.1"
                     /translation="MSAAHLTREPISPAALLADVAGPERGASCLFLGTVRDGPEDGGV
                     TGIEYSAYEAMAGAELERILAEAQRQWPLVRLAVRHRLGLVPTEDASIGIAAAAPHRA
                     DAFAACRYVIEEVKRRAPIWKKELRADGSVLWVDPQGRPAVVGPE"
     gene            complement(3516..3758)
                     /locus_tag="AC16JC_04045"
     CDS             complement(3516..3758)
                     /locus_tag="AC16JC_04045"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR5163901.1"
                     /translation="MIRLKFFARYAELLGREDLELPLPAPATVAEVVRQVRAGVPGGA
                     QLPERPLTAVNLRHVRLDATVRDGDEVAFLPPLAGG"
     gene            complement(3755..>4858)
                     /locus_tag="AC16JC_04050"
     CDS             complement(3755..>4858)
                     /locus_tag="AC16JC_04050"
                     /inference="COORDINATES: protein motif:HMM:NF017142.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HIR5163902.1"
                     /translation="TKLAETDGRIFGGLPYRKFVYFYLFRPAEAKAAVVEHGNSYVAI
                     VGAGAQAQPDDMVAQAAHEFFHAWNVKRIRPAEMWPYDYHAEQYTPLLWWSEGVTDYY
                     GDATNWRAGLWTDAQWLESVQSNIEQVQGAPEPWSEEDGSAATWINEVFVNSSQLYYP
                     KGSLTGLLLDISIRDATDNRHSLDEVTRNLYTRFYKQGKGFRTADLLALLTEAGLPDA
                     AGFYQKYVNGRDELPYEQILPKGGIAVQRHATSAPFLGVQTGGVANVVEAVVAGGAAE
                     AAGVQSGDTLLSVGDVKTPADQDWGPTFRDRYRGKAGTPLSIVVRRDGRPQTLTGTVI
                     ARTVNVFIVSKAASPDAKAARIFRGLATGTVGN"
BASE COUNT          690 a         1779 c         1666 g          723 t
ORIGIN      
        1 ggccggggtg aggacctcaa tcaggcgctg gaacgcgccg gccgcgtggc ggacttcatg
       61 gagttcgccg agctgatgtg cgaggacgcc ctgcagcgcg aggaatcctg cggcgggcat
      121 ttccgcgagg agtaccagac gcccgacggc gaggcgaagc gcgacgattc ccgcttctcc
      181 tacgtggccg cctgggaatg gaaaggcgtg gggacggccc cgcggccgca caaggagccg
      241 ctcgccttcg agcacgtcca cctggcccag cggagctaca agtgatgcgc ctcgtgctcc
      301 acgtctggcg gcaggccggc cccgaggccc cgggcaagtt cgtgcgctac gaggcgacgg
      361 acgtctcccc ggacatgtcg ttcctggaga tgctggacgt cctgaacgag acgctggtgg
      421 cgcgcaagga agagccggtg gcgttcgatc acgactgccg cgaggggatc tgcggctcct
      481 gcggcctggt gatcaacggc cggccgcacg gcccccaact cgccaccact gcctgccagc
      541 tgcacatgcg ccacttcacc gacggcgagg aggtctgggt cgagccgtgg cgcgcccgcg
      601 cgttcccggt gctcaaggac ctggtggtgg accgcggcgc gttcgaccgg atcatccagg
      661 cgggcgggtt catcacggcg cgcaccggta gcgcgccgga ggccaacgcc gtgccggtgc
      721 cgaagcgcga tgccgaccgc gccatggatg ccgcggcgtg catcggctgc ggggcgtgcg
      781 tcgccgcctg ccccaacgcc agcgccatgc tgttcgtggc cgcgaaggtg tcgcacctcg
      841 gcagcctgcc gcagggccag cccgagcgct acgagcgcgt ggtgaggatg gtggccacga
      901 tggacgaggc gggcttcgga gcgtgcacca accacggcga gtgcatggcg gcatgcccca
      961 aggagatctc ggtggactgg atcgcccagc tgaaccgcga cctcatgcac gcgacgctgc
     1021 ggccgccccg cgcgggcaag gactggagcg gggcggccta gcgcctagag cttcacgacc
     1081 gccatcgtct ccttcacggc attcgccgac ctctggagcg cggcccgctc ggcgtcggag
     1141 agcttcacct cgaggatctg ctccaccccg cccttcccca gtttgcacgg cacgccgcag
     1201 tagatccccg acaacccgta ctcgccctcg agccacgccg cgcacggcag gacccgcttc
     1261 ttgtcgagca cgatcgcctc ggccatctgt accgcggccg ccgagggggc gtagtaggcg
     1321 gagcccgtct tcaggaagtt cacgatctcg gcgccgccgt tgcgggtgcg cgtcacgatc
     1381 gcctcgagcg tcgcctgatc cagcagctgg ctcacgggga tccccgagac ggtggtgtag
     1441 gagacgagcg gcaccatggt gtcgccgtgg ccgcccagca ccatcgcctg gatgtcctcg
     1501 accgacacac ccagggcctc ggccaggaag ctgcggtagc gggcggtgtc gagcacgccg
     1561 gccatcccca ccacgcgctc gcgcgggaag ccggtgacct ccttcgccac ccagcacatc
     1621 acgtctagcg gattggagac catgacgacg atggcgcgtg gcgccacctg cttgatctgc
     1681 tgcgcgacct gcttgacgat ctcgacgttg gtgcgcacca ggtcgtcacg gctcatgccc
     1741 ggcttgcgcg cgatgcccgc cgtcacgatg aacagctcgc tgccctcggc gggctcgtag
     1801 gtgttcgtgc cgaccacgcg ggtgtcgaac ccctcgatcg gcgccgactc ccactgatcg
     1861 aggcccttac cctgcgggac gccctcgatc acgtccacca acaccaccgt gcgcgcgagg
     1921 ttcttctccg ccacgcgctg cgccgccgtg gcgcccacgt tgccggcccc caccaccgtg
     1981 atcttctgga acatgtgccg ctgccgtgcc tggaaagggt cggggaaagc taacccgcct
     2041 cagccgccgc gcgtatgcat ctcccggtgg gggtcggcgc gcaggccggt cacctggtac
     2101 agcggctgcc gctccgcgac ggccaggcgc tcctccgcgc cgcgatcgcg gcgtcccagc
     2161 caggtcgtcc gcaccagggc cgtgatctcg gcgtccgtgg ccccgtcacg cagcggcgca
     2221 cgcaggtcga cgccgcgctc ggcgtagagg cacagcagcc actgcccgtc cgccgtgagc
     2281 cggctgcgat cgcaggcgcg gcagaacggc gcggtggtcg aggcgatgat gccgaacgtc
     2341 gtgccgtcgg gcagcgcgaa ctgctcggcc ggcgcccggg gatcgtcgcc ggtgatcggc
     2401 gtgatggcgc cgtagtgatg cgccagccgg tccaggatct cccgctgcgg caccacccgg
     2461 tcgagcgacc agcgcgtcgc gccgccgacg tccatgtact cgatgaagcg cacttcggcg
     2521 ttgcgcgccc ggccgaactc gatcagatcg gtcagctcgt cgtcgttgaa tccgcgcagc
     2581 accaccgcgt tgagcttgag ccgcgcgaac cccagcgcct gcgccgcgcc gatgccggcg
     2641 agcacgtccg cgtgacgggc gctgcgggcg aagcgctcca tccggtcggg tcgcagcgta
     2701 tcgaggctga cggtgacgcg ccgcagcccc gcggcgcgca gctccggtgc atggcgcgcc
     2761 agcaggacgc cgttggtggt gagcgccagg tcgcgcaccc gcggctcgtc cgcgatcatc
     2821 cggaccagcg ccgggaggtc gtggcgcagc agcggctcgc cgccggtgag ccggaccttc
     2881 gtcgcgccca accccgcgaa gatccgcacc agtcgcgcga tctcctcgaa ggtgaggatc
     2941 tggtcgcgcg gcagccagac gtactcggcc tccggcatgc agtaactgca gcgcatgttg
     3001 cagcggtcgg tcaccgacac ccgcaggctg ccgagcgggc gtcccagcgt gtcgagcggg
     3061 gccgtcgtca ttcgggaccc accacggcgg gacgcccctg cgggtccacc cacagcacgg
     3121 agccgtcggc gcgcagctcc ttcttccaga tgggtgcgcg ccgcttcact tcctcgatta
     3181 cgtagcggca cgcggcgaag gcgtcggcgc ggtgcggcgc ggcggcggcg atgccgatgc
     3241 tcgcgtcctc cgttggcacg agcccgaggc ggtgccgcac ggcgaggcgg accaggggcc
     3301 attgccgctg cgcttcggcg agaatccgct ccagctcggc tcccgccatc gcttcgtacg
     3361 ccgagtattc gatgccggtc acgccgccgt cctccggccc gtcacgcacc gttccgagga
     3421 agagacagct cgcgccgcgc tcggggcccg ccacgtccgc cagcagggcc gctggggaga
     3481 tcggctctcg cgtcaggtga gccgcgctca tcgcgctacc ctcccgccag cggcggcagg
     3541 aacgcgacct cgtccccgtc gcgcaccgtg gcatcgagcc gcacgtgccg caggttcacg
     3601 gcggtgagcg gtcgctcggg cagctgcgca ccgcccggga ctcccgctcg gacctgccgc
     3661 accacttccg ccaccgtcgc gggcgccggc agcggcagct cgaggtcctc ccgccccagc
     3721 agctcggcgt agcgcgcgaa gaacttgagt cggatcaatt gcccaccgtc cccgtggcca
     3781 ggccgcgaaa gatacgcgcc gccttggcgt ccgggctggc cgccttggac acgatgaaca
     3841 cgttgaccgt ccgcgcgatc acggtgcccg tgagcgtctg cggtctcccg tcgcgccgca
     3901 ccacgatcga gaggggcgtc cccgccttgc cgcggtagcg gtcgcggaac gtcggccccc
     3961 aatcctggtc ggccggggtc ttcacatcgc ccaccgacag gagcgtatcc cccgactgca
     4021 ccccggccgc ttcggcggcg ccgccggcga cgaccgcctc gacgacattc gccacaccgc
     4081 ccgtctggac gccgaggaac ggcgcgctgg tcgcgtgccg ctggaccgcg atccccccct
     4141 tgggcaggat ctgctcgtag ggcagctcgt cccggccgtt gacgtatttc tggtagaagc
     4201 ccgcggcgtc ggggagcccg gcctcggtga gcaatgccag caggtccgcg gtgcggaatc
     4261 ccttcccctg cttgtagaac cgcgtgtaga ggttgcgggt cacttcgtcc aggctgtgcc
     4321 ggttgtcggt ggcgtcgcgg atcgagatat cgagcagcag acccgtgagc gatcccttcg
     4381 gatagtacag ctgggagctg ttgacgaaca cctcgttgat ccaggtggcg gcgctgccgt
     4441 cttcctcgct ccacggctcg ggcgcgccct gcacctgctc gatgttggac tgcacgctct
     4501 ccagccactg cgcgtcggtc cacaacccag cgcgccagtt ggtggcgtcg ccgtagtagt
     4561 ccgtcacgcc ctccgaccac cacaggagcg gcgtgtactg ctcggcgtga tagtcgtagg
     4621 gccacatctc cgccgggcgg atgcgcttga cgttccaggc gtgaaagaac tcgtgcgcgg
     4681 cttgcgccac catgtcgtcg ggctgcgcct gagcgccggc gcccacgatg gcgacgtacg
     4741 agttcccgtg ctccaccacg gctgccttcg cttccgcggg ccggaagaga tagaagtaga
     4801 cgaatttgcg atatggcagg cccccgaaga tcctgccgtc cgtctccgcc agcttcgt
//
LOCUS       DBNGZW010000163         1960 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1584531, whole genome shotgun sequence.
ACCESSION   DBNGZW010000163 DBNGZW010000000
VERSION     DBNGZW010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1584531"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1097
                     /locus_tag="AC16JC_04055"
     CDS             <1..1097
                     /locus_tag="AC16JC_04055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163903.1"
                     /translation="LRFGFLVFSRENAYGLDASRMTVAASSGGLEVTATGLVWAGGQE
                     RAPGKVVAHLRQDGDAVLWDVTAEMDQPIKAVTTVLRGVPRGRISSGGGEPFDPKDDE
                     VLFGYPFGGGDLFGGNVAWGMGTPLVVVQAGAQRFLALSSLDDRVRTKRFLFQPGEAG
                     YRVELVHEVEGWLDQPRVQVPTWRAGYATTLESAVAPHYAHLERAYRLPRWESRADVP
                     AWLRRTALVVTLHGMHYTGYVFNDYARMLEILQWIATRIPPERVLAFISAWDGRYYWD
                     YPSYRAADRLGGEAGLRRLVQQGQRLGFRFMPMFGANAANRRRPEYPGIADASVAKVD
                     GDRLDINWVDWDNDRHREGWLSYMNLGVDS"
     gene            1170..1559
                     /locus_tag="AC16JC_04060"
     CDS             1170..1559
                     /locus_tag="AC16JC_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163904.1"
                     /translation="MGGWTNNTRADMHEGTRKLVADLRARYPQVLACGEFLYDALLEF
                     IPLYHVHNPRAVPFARFFSHLSHPAPGRGSSGVHESGFSSWDAATLSLSRREGLIPTL
                     MVVDDTFTKYRDAMAAVIARAKEWGSG"
     gene            1556..>1960
                     /locus_tag="AC16JC_04065"
     CDS             1556..>1960
                     /locus_tag="AC16JC_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-L-arabinofuranosidase domain-containing
                     protein"
                     /protein_id="HIR5163905.1"
                     /translation="MRRREFLAATAAAALPLYRLGRISPLHAEGIVSPVRPFDLSAVR
                     LLPGPFLDAMAVNRRFLLAQDPDRLLHMFRLTAGLPSTAEPLGGWEAPDNELRGHYTG
                     HYLSALALMSASQGDSELKARGDRLVAELWKCQ"
BASE COUNT          289 a          671 c          684 g          316 t
ORIGIN      
        1 gactgcgctt cgggtttctc gtcttctcac gggagaacgc ctacggcctc gacgcgagcc
       61 gcatgacggt cgcagcctcg tcgggcggcc tcgaggtgac ggccacgggg ctcgtctggg
      121 caggcggtca ggagcgcgca ccgggaaagg tggtcgccca ccttcggcag gacggcgacg
      181 ccgtcctctg ggacgtcacc gccgagatgg accagccgat caaggcggtc acgaccgtgc
      241 tgcgcggcgt gccgcgcggc aggatctcga gcggcggcgg cgagccgttc gaccccaagg
      301 acgacgaggt cctgttcggc tatccgttcg gcggcggcga cctgttcggc ggtaacgtcg
      361 cgtggggcat ggggacgccg ctcgtcgtcg tccaggccgg cgcgcagcgg ttcctggcgc
      421 tctcctcgct cgacgatcgc gtgcgcacca agcggttcct gttccagccg ggagaggcag
      481 ggtaccgcgt cgagttggtc cacgaggtcg aggggtggct cgaccagcca cgagtgcagg
      541 tccccacctg gcgcgcgggt tacgccacga cgctcgagag cgcggtggca ccgcactacg
      601 cgcacctgga gcgggcgtac cgactgccgc gctgggagtc ccgggccgac gtgccggcct
      661 ggctgcggcg cacggccctg gtcgtcacgc tgcacggaat gcactacacg ggctacgtgt
      721 tcaacgacta tgcccggatg ctcgagatcc tccagtggat cgcgacgcga atcccgccgg
      781 agcgcgtgct cgccttcatc tcggcctggg acgggcgcta ctactgggac tacccctcgt
      841 accgcgccgc cgaccggctc ggcggcgaag cgggcctccg gcgactggtc cagcaaggcc
      901 agcggctcgg cttccggttc atgccgatgt tcggggcgaa cgcggccaac cgccgccggc
      961 ccgagtaccc gggaatcgcg gatgcgagcg tggccaaggt ggacggggac cggctcgaca
     1021 tcaactgggt ggactgggac aacgaccgcc atcgggaggg ctggctctcc tacatgaacc
     1081 tcggtgtgga ctcgtagcgc gcctggctca ccgcccgcat cgccgacgcc atcgagcgct
     1141 atggggtgga tgcctatttc ttggacattg tgggcgggtg gacgaacaac accagggcgg
     1201 acatgcacga ggggacacgc aagctcgtgg ccgacctgcg ggcgcggtac ccgcaggtgc
     1261 tggcgtgcgg tgagtttctc tacgacgcgt tgctcgagtt catcccgctc tatcatgtcc
     1321 ataacccaag ggccgtgcca ttcgcgcggt tcttctcaca tctttctcac cccgcgcccg
     1381 gacggggatc gagcggcgtg cacgaatcgg ggttcagctc ctgggacgcg gccacgctat
     1441 cattgtcccg gcgggaagga ctgatcccga ccctgatggt cgtggacgac accttcacca
     1501 agtatcggga cgcgatggcc gcggtgatcg cacgagccaa ggaatggggg tcgggatgag
     1561 acgccgcgag tttctcgccg ccacggcggc cgccgcactt cccctgtacc gcctcggccg
     1621 aatcagcccg ttgcatgccg aaggcattgt gtccccggtc cgcccgttcg atctctcggc
     1681 cgtccggctg ctcccgggtc ctttcctcga cgcgatggcg gtgaaccgcc gcttcctgct
     1741 ggcgcaggat cccgaccggc tgttgcacat gttccgtctc acggcgggtc tcccctccac
     1801 cgccgagccg ctgggcggat gggaagcgcc cgacaacgag ctgcgcggcc actacaccgg
     1861 ccactacctc tcggccctgg cgctgatgtc ggcgagccag ggcgacagcg agctcaaggc
     1921 tcgcggggac cggctcgtgg ccgagctgtg gaaatgccaa
//
LOCUS       DBNGZW010000164         1840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_627935, whole genome shotgun sequence.
ACCESSION   DBNGZW010000164 DBNGZW010000000
VERSION     DBNGZW010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_627935"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..812
                     /locus_tag="AC16JC_04070"
     CDS             81..812
                     /locus_tag="AC16JC_04070"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020714778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HIR5163906.1"
                     /translation="MQGTKPGFTQQDWSAYTEEEHGVWGLLYERRMASLRHTGSRLFL
                     EGAQWIGLRPDRVPDLADVNTRLAARTGWSAVPVTGFLPAREFFGCLAQRRFPTTVTV
                     RPRSQLDYLPEPDIFHDVFGHVPLHAHPVFADFLQRFGRAASAAVTDEQVEAMARLFW
                     FTVEFGLIREGTETRVYGSGLISSHGDAGNALGPDCDRRPFTLDGVLVQPFEIDHFQE
                     VLFTIDSFEQLFAAVAEAEHRLGLH"
     gene            863..>1840
                     /locus_tag="AC16JC_04075"
     CDS             863..>1840
                     /locus_tag="AC16JC_04075"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5163907.1"
                     /translation="MRAFAVAACGALLGVTALAAQQQWVGPQPPCDIKPGFFRINSVI
                     VDLQKAVRTPALRDRMLSQAQDVLMRTIRDDKQDKNPAAWYYLGRYYVEMKDGAGADT
                     AFDRVEALAPQCKADIAGYRGQLAEAATNAGLTLWHGGNADSGAVLLRQAYAVDPANP
                     KPLFQLGSLYAERNQLDSAAAILGRAAAASGSDPAYADAKREALLTVARLAQRNLQAN
                     PAVQKWQHSRYSRDSLTPYLAADSGVLARMQQSSASRRARGARLSPADQQAFNRDSTT
                     RADAVARGRALRETLQQQAAADSATAQAAFDPAVAAYRGLVAAYPTNVEA"
BASE COUNT          284 a          639 c          625 g          292 t
ORIGIN      
        1 gtaacccagc gatcccggac ggcccgtgag tcatctcacg ggccgttgtt tcttgtcccc
       61 gttcccctcg aggaggtccc atgcagggca cgaagccagg tttcacgcag caggattggt
      121 cggcctacac cgaggaggag cacggcgtgt ggggcctcct gtacgaacgt cgcatggcgt
      181 cgctgcggca cactggcagc cggttgtttc ttgagggggc ccagtggatc ggcctgcggc
      241 ccgaccgggt accggacctg gcggacgtca acacccgtct ggccgcccgc accggctggt
      301 ccgccgttcc ggtcacggga ttcctgccgg cgcgggagtt cttcggatgt ctggcgcagc
      361 ggcggtttcc cacgacggtc acagtgcgcc cccggagtca gctcgactac ctcccggagc
      421 cggacatctt ccacgacgtg ttcggccacg tcccgttgca cgcccacccc gtattcgccg
      481 atttcctcca acgcttcggg cgcgcggcga gcgccgccgt cacggacgag caggtcgagg
      541 cgatggcgcg gctgttttgg ttcacggtgg agttcggcct gattcgggaa ggcactgaga
      601 ccagagtcta cgggagcggc ctcatctcct ctcacggcga cgcgggcaac gccctggggc
      661 ccgactgcga ccggcgcccc ttcacgctgg atggggtgct cgtgcagccg ttcgagatcg
      721 accacttcca ggaggtgctc ttcacgatcg actcgttcga gcagttgttc gccgcggttg
      781 cggaggccga gcaccgtctc ggactgcatt gacaccccgg cgcggccggg cagattcgac
      841 caaaactcga gtggagcagg atatgcgtgc gttcgccgtc gccgcctgcg gggcgctgct
      901 gggcgtcacc gcccttgccg cgcaacagca atgggtgggg ccgcaacccc cgtgcgacat
      961 caagcccgga ttctttcgca tcaacagcgt catcgtggat ctgcagaagg ccgtccggac
     1021 cccggcgtta cgggatcgca tgctgtctca ggcacaggac gtactgatgc ggacgatccg
     1081 cgacgacaag caggacaaga acccggccgc ctggtactac ctggggcgct actacgtcga
     1141 gatgaaagac ggagcgggcg ccgacaccgc cttcgaccgc gtggaagcac tcgcgccgca
     1201 gtgcaaagcg gacattgccg ggtaccgcgg gcagctggcc gaagccgcca ccaatgcggg
     1261 actcacgctc tggcacggcg gcaatgcgga ctcgggggcg gttctcctgc gccaggccta
     1321 tgccgtggat ccggcgaacc cgaagccgct gttccagctc gggagtctgt acgcggagcg
     1381 taatcagctc gattccgcgg cggcgatcct gggccgcgcc gcggcggcgt cggggtcaga
     1441 cccggcctac gccgacgcga agcgggaggc gctgctcacg gtggcccggc tggcgcaacg
     1501 gaacctgcag gcgaatcccg ccgtccagaa atggcagcat tcccgctaca gccgcgattc
     1561 gctcactccc tacctggccg cggattctgg cgtcctggcc cggatgcagc aatcgtcggc
     1621 atcgcgccgc gcgcgggggg cacggctctc tcccgcggac caacaggcgt tcaaccgcga
     1681 ctcgactacg cgtgcggatg ccgtcgcgcg cgggcgcgcc ctgcgcgaga ccctgcagca
     1741 gcaggcggcg gccgacagcg cgacggccca ggcggcgttc gatccagccg tggccgccta
     1801 tcgtggcctc gtcgccgcct atcccaccaa tgtggaggcg
//
LOCUS       DBNGZW010000165        11309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1651011, whole genome shotgun sequence.
ACCESSION   DBNGZW010000165 DBNGZW010000000
VERSION     DBNGZW010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 11309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11309
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1651011"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            197..2404
                     /locus_tag="AC16JC_04080"
     CDS             197..2404
                     /locus_tag="AC16JC_04080"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5163908.1"
                     /translation="MERGLQGRYAIERELGQGGMALVFLARDLRHERKVALKVLRPEI
                     SAEIGAERFLREIKMAAGLTHPHILPVYDSGEADGLLFYVMPNMEGRSLRERLDRQRQ
                     MPLDEALRITREVAAALDYAHRHQVVHRDVKPENILLHEGAAMVADFGIGKALSGNGS
                     ITQTGMALGTPAYMSPEQAGGEADIDGRSDLYSLGCVLYEMLSGEPPFTGPTAQAVIA
                     KRFVAPIPKVRATRDVPEVVDDAITRALARTPVDRFPTAAEFAEALLPGRRTPPEAPK
                     GAPPTKAVAVIPLANMSADPENEYFSDGMTEEIINALAKVPGIQVASRTSSFAFKGKE
                     VDIRQIGDKLGVGSVLDGSVRKVGNRIRITVQLINVENGYHIWSETYDRQLEDVFAIQ
                     DEISRAIVEALKLRLGGEAGPLVVPTKNIEAYNIYLKARFFFNKFTEPGLRKALEFYQ
                     LALLQDPSFARAYAGIADVWCNLADDWVAPDDAYPRAKAAAERALQREPDLAEAITSV
                     GKVLCWHEWDFAGAERQLERAVRLSPNYAEAHYVLGTALPLVGRLSEAIEEIRRALVL
                     DPLSAHFSSWLARFMLYAGDYEGAIAQGHKTLELDEGSIRAPHYIGSAHLAQGDAETA
                     LQWYRRAQGLEKAVRSYDAMIVRALAALGRRDEAEAILARLDEESRQQYVRSEILAMG
                     YAAMGDLDHAFACLERAFQARSAGLIYLHLDPGYAPLRGDPRYADLVRRIGLK"
     gene            complement(2417..3733)
                     /locus_tag="AC16JC_04085"
     CDS             complement(2417..3733)
                     /locus_tag="AC16JC_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5163909.1"
                     /translation="MRVLLRGGAVLLLGAACRGPQAGAPPAPTPPPQADACVLSGDST
                     IPPPDSLTLALPGAVDPAHAPAPETEAERMIFRQVFETLVRLDCAGAPRSGLAQSWSA
                     SDSGVQWTFTLRAGARFQDGQAVTAPEVVASWMSSDTTLLEGATVAAPDDRTLVLRLR
                     HPSPAPPLWLAHPALAVRRTIPGRDWPVGTGPYTADTAAGGAVLRPFTGERRPTLLLP
                     RLGAGDARDWLDRGFDLVVTNDAAALGYAASRPELATRPLPWDRTYLLLAPGAAPPVT
                     TAWREGLARDAVRVEARASAAPAWWTAFPACIAPAPAGAAPVPPASSRLLVVYPRDDA
                     TARSIAERLVALEGAQAGTTARAAGLGPVELTGALAAGRTGQFVIPFPQGVLEPCRAV
                     HELVARAPWLTLASQIVPLIDTRRRVVWRRGAAAFTVDWDGTLRVR"
     gene            complement(3742..6543)
                     /locus_tag="AC16JC_04090"
     CDS             complement(3742..6543)
                     /locus_tag="AC16JC_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013926146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HIR5163910.1"
                     /translation="MSRRLPSLVLCLAVAVPAASLAAQQTADTAYARLVREYTTDSRF
                     LPASVATLPLSTAIPSPQRHFGTIAGAPGVMHHSGEIYGYFRALAKATPRVRVETVGK
                     TEQGRDIVLVVIADSTTLARTDAYRATLARLADPRTVPAASLDSVLAVAKPVYYLNGG
                     LHSTEMGSPEMLMELAYRLAASDEPAMRRIRDGVITLINPVSEPDGRDRQVDWYLHYT
                     KSHATPDDGFPRSSPFWGDYAFHDNNRDGLQMALALTRAIYKVYYDWHPVVMHDLHES
                     IALLYISTGTGPYNEHNDPVAVSEWQLFANNDITALDAQGLPGVWTWAFFDGWWPGYA
                     MWVANNHNAIGRFYETFGNAGANTYVRDLSDESYAGDSVTSRQWYRPWPPTKKVRWSA
                     RDNVNYMEAGVLASLSFTAENAERLLRNFWQKGTNSIARGRTEKPYAYLIPGFARQRD
                     PSRAAYLVNQLERQRIEIQRRTTGDSAGDFVILRDQPYGDLVANLLGTQHYPQSAKYP
                     PYDDIAWTLGDQYGVTVTGVNDTAVFHWAGLERVIDTVAAPGVVTGSSGSVYVVGYRG
                     QVDVVPGLYALRDLAPGVRAFAAETAFVAGSDTFPAGSVVIEGAHPEPMRIVAQRYGL
                     TIQRMTTAPTVARHLLDLPRVAIYHSWYSTQDAGWARYTFEQYGIPYTSIDKDDLRRG
                     GLRPRFDVILVPSFGGAVTQMVNGVDRKWGPLPYQQTKDTPNLGTPFSSPDITGGPGF
                     AGVAELQRFVDAGGTLITLGAATTLASETGIARPLSPHRTSTLFHPGSIVRARAVQRR
                     SPILYGYPDVTTVFRGNGPLFEVADRDSAFVVLRYGATKKDERDEGPMLGIPDTAPPP
                     PHAKPDSTKAAPSREEAYVVSGMVRNEREIVGEGAIFDVPVRSGRVIAFSFDPLHRFL
                     NHHEFPLVWNALANWNDRP"
     gene            complement(6619..9096)
                     /locus_tag="AC16JC_04095"
     CDS             complement(6619..9096)
                     /locus_tag="AC16JC_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR5163911.1"
                     /translation="MALEDLRYAVRGLKSRPGFTVAVVLTLALGIGANTAMFSVVDRL
                     LFRPPPLLRDPALVHRVYLARSYRGEEFTGDGVQYARYLDLTRDTRSFARTATFADRQ
                     LAIGEGTDSREMQVGVVSAGFFGFFDAPPVLGRYFTTTEDSPPNGAAVTVLGYGFWQT
                     RYGGRRDVLGQTLRIGATLYTVIGVAPRDFVGLWSGRPPAAYIPVTTYGGELAANLRL
                     RGETWWTTYHWTWASMLVQRKPGVTLEAANADLTTAYRKSYMTQDAADHGGMTPIALA
                     RPHAIAASVLSERGPNQSSETKVATWITGVALVVWLIACANVANLLLARALRRRREIA
                     VRLAMGVSRARLVSQLLTESLLLALLGGTAGVLVAQWGGAVLRAALLRDTTGSAVATD
                     PRTLLFAGAAALVAGFLTGLAPILQTRRADLTQDLKAGEREGAVHRSRTRATLLVLQG
                     ALSVVLLIGAGLFVRSLRHVQHVHLGYDADSLLVIDLQMRGMHLDSAQNVSLLRRLLA
                     ETQAIPGVSHASRQVTMPFWSSWNTDLHVAGIDSVDRLGEFELNAVSGDYFATMGTRL
                     LRGRGVTDQDVAGAPRVMVVSEAMAQALWPGREPLGECVRVGADTAPCTGVVGVAENI
                     KSRSLGHDPGLFYYMSSAQFNPEMGGLFVRVQGAGSRFKETVRRRLQPLMPGASYVTV
                     TPLADVLGRQTQSWELGATMFTLFGVLALALAAIGLYSVIAYNVAQRTHELGVRSALG
                     AQLGDLVRLVLTDGMRLALVGVVLGSVIAFIVGKWAQPLLFEQSARDPAVFAGVAGLL
                     LAVAALACFIPARRAGRVDPMRALRSE"
     gene            complement(9132..10514)
                     /locus_tag="AC16JC_04100"
     CDS             complement(9132..10514)
                     /locus_tag="AC16JC_04100"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR5163912.1"
                     /translation="MRKALVWVGIVLAGRTVPLQSQQPVGPMVDSIARQVLKASGAPS
                     ASVAVVQEGRLVYAQAYGSARVEPPVVARAEMRYSIGSISKQFCATAILLLQQEGKLS
                     LDDPVGKFLPDLTRARDVTIRQLLSHTSGYQDYWPQDYVPLFMLDSVTAQGILERWAR
                     KPLDFEPGTQWQYSNTNYVAAGLIVEKASGMPFFEFLRTRILAPLGLQSVVNIDLGRL
                     PQSDPTGYQRFALGPPRAAPKEGKGWLFAAGELAMTASDLAKWDITLMNESLLSPASY
                     RELETETLLKNSTATGYGLGVDVGMNAGHRVISHGGEVSGFTATNLVFPDEHAAVIVL
                     VNQDAVSTSGAIAQRIAGLLFATGDTLTASRTARARRIFEGLQHGTIDRTLFTADGNA
                     YFSAQAVADFASSLGPLGALQGFVQTAQQRRGGMVYRSYRASFTDRSLRVWTFELPDG
                     KLEQYQVAPT"
     gene            10576..>11309
                     /locus_tag="AC16JC_04105"
     CDS             10576..>11309
                     /locus_tag="AC16JC_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR5163913.1"
                     /translation="MTKLTVRLALGAALLAPNAAPAQQPAAGSPQTPAPIRAGVPDTS
                     PFRMLPLPPPDVYRAASGMPGPSYWQQRADYTIRASLDTATHTIRGEETIRYTNNSPD
                     TLRFVWLQLDMNAGSPDTRFTALANPFARTEPGFRAGATIERVAALHSASPRASPVAT
                     PLTWRVNSTMMRVDLDRPLPPHGTVSLAVAWHHQIPRFGRTGRQQFPQGWLYQVAQWY
                     PRLAVYDDVRGWNTDQYIGSSEFYLE"
BASE COUNT         1806 a         3977 c         3918 g         1608 t
ORIGIN      
        1 gccgctggcg caccgcgcga cgcacgccga gcccgaacac gacgagcggc acgagcacca
       61 ccggcgcggt tgcgatgagg atgcgcgtcc gaaaggtcat cggggtggaa acgatagcat
      121 tccttcatac cccccgctgg ccccagccca ggcgatgcgg catccttctc attctatggc
      181 ggaatttcaa gagcgtttgg agcgcggact acagggccgc tacgcgatcg agcgggagct
      241 gggtcagggc ggcatggccc tggtgtttct cgcccgcgac ctgcggcacg agcggaaggt
      301 cgccctgaag gtgctgcgac cggagatctc ggccgagatc ggagccgagc gcttcctgcg
      361 tgaaatcaag atggcggccg gactcaccca tccccacatc ctcccggtgt acgactccgg
      421 cgaagccgac ggcctgctct tctatgtgat gccgaacatg gagggacgct cgttgcggga
      481 gcggctcgac cggcagcggc aaatgccgct ggacgaagcg ctccggatca cgcgggaggt
      541 cgccgcggcg ctggactatg cccaccgcca ccaggtcgtt caccgggacg tcaaaccgga
      601 gaacatcctg ctgcacgagg gcgcggcgat ggtggccgac ttcggcatcg gcaaggcgct
      661 gagcggcaac ggatcgatca cgcagacggg gatggcgctg ggcactccgg cgtacatgag
      721 cccggagcag gcgggcggcg aagccgacat cgacggccgc agcgatctct acagcctcgg
      781 ttgcgtgctg tacgagatgc tgtcgggcga gccgccgttc accggcccga ccgcccaggc
      841 ggtgatcgcc aagcggttcg tcgcgcccat cccgaaagtg cgcgctaccc gcgacgttcc
      901 cgaggtggtg gacgacgcca tcacccgggc cctcgcgcgc acgcccgtgg accgcttccc
      961 cacggcggcc gagttcgcgg aagccctgct gccgggacgg cgcacgccgc cggaggcgcc
     1021 aaagggcgcc cctcccacca aggccgttgc cgtcattccg ctcgcgaaca tgagcgccga
     1081 tcccgagaac gagtacttca gcgacgggat gacggaagag atcatcaacg cgctcgcgaa
     1141 agtgccgggc atccaggtgg cgtcgcgcac ctcctccttc gccttcaagg ggaaggaagt
     1201 ggacatccgg cagatcggcg acaagctcgg ggtggggtcg gtgctcgatg gcagcgtgcg
     1261 gaaggtgggg aaccgcatcc gcatcaccgt ccagctgatc aatgtcgaga atggttacca
     1321 catctggtcg gagacctatg accgccagct cgaggacgtc ttcgcgatcc aggacgagat
     1381 ttcacgagcc atcgtcgagg cgttgaagct ccgcctcggc ggcgaagccg gcccgctcgt
     1441 cgtgccgacc aagaacatcg aggcgtacaa catctacctc aaggcgcgct tcttcttcaa
     1501 caagttcacc gagcccggcc ttcgcaaggc cctcgagttc taccagctcg ccctgcttca
     1561 ggacccctcc ttcgcccgtg catacgcggg catcgcggac gtctggtgca acctcgccga
     1621 cgactgggtg gcgccggacg acgcctatcc gcgagcgaag gcggcggccg agcgggcgct
     1681 gcagcgggag cccgaccttg ccgaggcgat tacctcggtc ggcaaggtcc tgtgctggca
     1741 cgagtgggac ttcgccgggg ccgagcgcca gctcgagcgg gcggtccggc tcagtccgaa
     1801 ctatgccgag gcccattacg tcctcggcac ggcgttgcct ctggtggggc gcctgagtga
     1861 ggcgatcgag gagatccgca gagcgctcgt gctcgatccg ctgtcggccc atttcagctc
     1921 ctggctcgcc cgcttcatgc tctacgcggg ggattacgag ggcgcgattg cgcaggggca
     1981 caagaccctg gaattggacg agggctcgat ccgtgcaccg cactacatcg gctcggccca
     2041 cctggcccag ggcgacgccg agaccgccct ccagtggtac cgccgtgcgc aggggttgga
     2101 gaaggccgtc cgctcctacg acgccatgat cgtgcgcgcc ctcgccgcac tgggtcgccg
     2161 ggacgaggcg gaggcgatcc tggcccggtt ggacgaggaa tcgcggcagc agtacgtgcg
     2221 cagtgagatc ctggcgatgg gctacgcggc gatgggcgac ctggaccacg ccttcgcttg
     2281 cctcgagcgc gcctttcagg cccgctcggc cggtctcatc tacctccatc tggacccggg
     2341 atacgcgccg ctgcgcggcg acccccgcta cgcggacctg gtgcggcgca tcggcctgaa
     2401 ataggagcgg cccccgtcac cggacgcgga gcgttccatc ccagtccacg gtgaacgccg
     2461 ccgcgccgcg gcgccatacc acccggcgcc gcgtgtcgat cagcggcacg atctgcgacg
     2521 cgagcgtgag ccacggcgcg cgcgccacca gttcgtgcac cgcacggcac ggctccagca
     2581 ctccctgcgg gaatggaatc acgaactgcc cggtccgacc cgcggcgagc gcccccgtca
     2641 actcgacggg tcccagcccc gccgcgcgtg cggtagtacc ggcctgagct ccttcgagcg
     2701 ccacgaggcg ctcggcgatg ctgcgcgccg tcgcgtcgtc gcgtggatac acgacgagca
     2761 agcgcgagct cgcaggagga acgggggccg cgcccgccgg tgcgggggcg atgcaggcgg
     2821 ggaacgccgt ccaccaggcc ggagctgctg aggcacgcgc ctcgactcgc acggcatcgc
     2881 gagccagacc ctcccgccac gcggtcgtaa ccgggggcgc cgcgcccggt gcgagcagca
     2941 ggtaggtacg atcccagggc agcggtcgcg tcgcgagctc ggggcggctc gccgcatagc
     3001 ccagcgccgc cgcatcgttc gtgacgacga ggtcgaagcc gcgatcgagc cagtcgcggg
     3061 catcgcccgc gcccagccgc ggcagcagca gcgtcggacg tcgctctcca gtgaacggcc
     3121 gaaggacggc tccgccggcg gcggtatccg ccgtgtacgg cccggtgccc accggccagt
     3181 cccgtccggg aatcgttcgc ctcacggcaa gcgcgggatg ggccagccag agcggcggcg
     3241 ccggcgaagg gtgcctgagc cgcagcacga gcgtacgatc gtcgggagcg gcgaccgtgg
     3301 cgccttcgag cagcgtcgtg tcgctgctca tccaggacgc gacgacctcg ggcgccgtca
     3361 cggcctggcc gtcctggaaa cgcgcgcccg cgcgcagcgt gaacgtccac tgcacgccgg
     3421 agtccgaggc cgaccacgac tgcgcgagac ccgagcgcgg ggcgcccgcg caatcgagcc
     3481 gcaccagcgt ctcgaacacc tgccggaaga tcattcgctc cgcttcggtc tcaggtgccg
     3541 gggcgtgggc cgggtcgacc gctcccggga gcgcgagcgt cagggagtcg ggggggggga
     3601 tcgtcgagtc acccgacagc acacaggcat ccgcctgcgg cggtggcgtc ggagccggcg
     3661 gcgcaccggc ctgcgggccc cggcaggcgg caccgagcag cagcacggcg ccgccccgga
     3721 gcaacacgcg catgcgacga ttcagggccg gtcgttccag ttcgccagtg cgttccacac
     3781 gagcggaaac tcgtgatggt tgaggaagcg gtgtaacggg tcgaacgaga atgcgatgac
     3841 ccgtccgctg cgcacgggca catcgaagat cgcgccctcg cccacgatct cgcgctcgtt
     3901 gcgcaccatc cccgaaacga cgtacgcctc ctcccgggac ggcgcggcct tggtactgtc
     3961 cggcttcgcg tgcggcggcg ggggcgccgt gtcggggatg ccgagcatcg gcccctcgtc
     4021 gcgctcgtct ttcttcgtgg cgccataccg aaggacgacg aacgcgctgt cgcggtcggc
     4081 cacctcgaac agcggcccgt taccgcggaa caccgtggtg acgtcgggat agccgtagag
     4141 aatcggcgag cggcgctgca ccgcccgcgc gcgcacgatc gagccgggat ggaacagcgt
     4201 gctcgtgcgg tggggcgaca ggggacgggc gatcccggtc tcggacgcga gcgtggtggc
     4261 cgcgcccagc gtgatgagcg tgcccccggc gtccacgaac cgctgcagct ccgccacgcc
     4321 cgcgaacccg ggcccacccg tgatatcggg cgaggagaag ggcgtgccga ggttgggggt
     4381 gtccttcgtc tgctggtagg gtaacggccc ccatttgcgg tcgacgccgt tgaccatctg
     4441 cgtcaccgcg ccgccgaagc tgggaaccag aatcacgtcg aagcgcgggc gcagcccccc
     4501 gcggcgcagg tcgtccttgt cgatcgaggt gtaggggatg ccgtactgct cgaaggtgta
     4561 gcgcgcccaa cccgcgtcct gcgtcgagta ccagctatgg tagatcgcca cgcgcggcag
     4621 atcgaggagg tggcgtgcca cggtcggcgc ggtggtcatg cgctggatcg tcagaccgta
     4681 gcgctgggcg acgatgcgca tgggttcggg gtgggcgccc tcgatcacga ccgatccggc
     4741 cggaaaggtg tccgacccgg cgacgaacgc cgtctcggcc gcgaacgccc tgactcccgg
     4801 cgccaggtcg cgcagcgcgt acagcccggg gacgacgtcg acctgtccgc ggtaaccgac
     4861 gacgtatacg gacccgctgc tccccgtcac cacccccggc gccgcgaccg tgtcgatcac
     4921 ccgctcgagc cctgcccagt ggaacacggc ggtatcgttg acgccggtga ccgtcacgcc
     4981 gtactggtcg ccgagcgtcc aggcgatgtc gtcgtacggc gggtacttgg ccgattgcgg
     5041 gtagtgctgg gtccccagca ggttggcgac gaggtcgccg tacggctggt cgcgcaggat
     5101 cacgaagtcg ccggcggaat cgccggtcgt gcggcgctgg atttcgatac gctggcgctc
     5161 gagctgattg acgagatagg cggcgcggga tggatcgcgc tggcgggcga agccggggat
     5221 gaggtaggcg tagggcttct cggtgcggcc gcgcgcgatg ctgttcgtcc ccttctgcca
     5281 gaagttgcgc agcaggcgct cggcgttctc ggcggtgaac gagagcgacg ccagcacgcc
     5341 cgcctccatg tagttcacgt tgtcccgcgc cgaccagcgg accttcttcg tgggcggcca
     5401 ggggcggtac cactgccgac tcgtgaccga gtccccggcg tagctctcgt ccgacaaatc
     5461 ccgcacgtag gtgttggcgc cggcgttgcc gaacgtctcg tagaagcggc cgatggcgtt
     5521 gtggttgttc gcgacccaca tcgcgtaccc cggccaccag ccgtcgaaga acgcccaggt
     5581 ccagaccccg gggagcccct gcgcgtcgag cgccgtgatg tcgttgttgg cgaacagctg
     5641 ccactcgctg accgccacgg gatcgttgtg ttcgttgtac gggccggtgc cggtggagat
     5701 gtagagcaac gcgatggact cgtgcaggtc gtgcatcacg acggggtgcc agtcgtagta
     5761 caccttatag atcgcgcggg tgagcgccag cgccatctgc agcccgtccc ggttgttgtc
     5821 gtggaaggcg tagtcgcccc agaacgggct ggagcggggg aagccgtcat ccggcgtcgc
     5881 gtgactcttg gtgtagtgca gataccagtc cacctggcgg tcgcggccgt cgggctcgct
     5941 caccgggttg atcagggtga tcaccccgtc gcggatgcga cgcatggccg gctcatcgct
     6001 cgcggcgagc cggtacgcca gctccatcag catctccggg ctccccatct cggtcgaatg
     6061 caagccgccg ttcaggtagt agaccggctt ggcgacggcg agcaccgaat cgagcgaggc
     6121 cgcgggcacc gtccgtgggt cggccagccg ggcgagcgtc gcccggtagg cgtcggtccg
     6181 cgccagcgtg gtagaatcgg cgatgacgac gagcacgatg tcgcgcccct gctcggtctt
     6241 ccccaccgtc tccacccgca cccgcggcgt ggccttcgcc agcgcgcgga agtagccgta
     6301 gatctcgccc gaatggtgca tgacccccgg ggcgccggcg atcgtgccga agtggcgctg
     6361 cggcgagggg atcgccgtcg agagcggcag cgtcgccacg gaggccggga gaaaccggga
     6421 gtcggtcgtg tattcgcgca ccagccgcgc atatgccgta tcggcggtct gctgggcggc
     6481 cagagacgcc gcgggaacgg ccacggcgag gcacaggacg agcgacggca gacggcggga
     6541 catcgaacct ccgagcaagc ggtgagtgcc gagcatccca ccgccaaagc tgcaggctca
     6601 gaggcggacg cgcaaaacct attccgaacg caaggcccgc atcgggtcca cgcggcccgc
     6661 ccggcgcgcg gggataaagc aggccagcgc ggcgaccgcc aggagcagcc ccgccacgcc
     6721 ggcgaacacc gccggatccc gggccgattg ctcgaacaag agcggctgcg cccacttgcc
     6781 tacgatgaat gcgatgacgc tgcccaggac gacgcccacc agagccagcc gcatgccgtc
     6841 ggtgaggacg aggcgcacca ggtcgcccag ctgggccccc agcgccgagc ggacgcccag
     6901 ctcgtgcgtg cgctgggcga cgttgtaggc gatcacgctg tacaggccga tcgccgcgag
     6961 cgccagcgcc agcaccccga atagggtgaa catcgtggcg cccagctccc acgattgtgt
     7021 ctgcctgccg agcacatcgg cgagcggtgt cacggtgacg tacgacgccc ccggcatgag
     7081 cggctgcagg cgccgtcgca ccgtctcctt gaaccgggag ccggcgccct ggacccgcac
     7141 gaagagcccg cccatctcgg gattgaattg cgccgacgac atgtaataga agaggccggg
     7201 gtcgtggccc aggctccggg atttgatgtt ctcggccacg ccgaccacgc ccgtacacgg
     7261 cgccgtgtcc gcgcccacgc gcacgcattc gcccagcggc tcgcggcccg gccacaatgc
     7321 ctgggccatc gcctcgctca cgaccatcac gcgcggcgcg ccggcaacgt cctggtcggt
     7381 gaccccccga ccgcgcagca ggcgcgtgcc catggtcgcg aagtagtctc cggagacggc
     7441 attcagctcg aactcgccga gtcggtccac ggagtcgatg ccggccacgt gcagatccgt
     7501 gttccagctg ctccagaacg gcatcgtgac ctggcgcgag gcgtggctga ccccgggaat
     7561 ggcctgggtc tccgcgagca gccgccgcag cagcgacaca ttctgcgcgc tgtcgagatg
     7621 catcccgcgc atctggagat cgatgaccag cagcgagtca gcgtcgtagc ccaggtgcac
     7681 gtgctgcacg tgccgcagcg agcgcacgaa cagcccggcg ccgatcagca gcaccaccga
     7741 gagcgccccc tgcagcacga gcagcgtggc ccgcgtccgg gagcggtgga cggcgccctc
     7801 gcgctccccc gctttcagat cctgcgtgag gtcggcgcgg cgcgtctgga gaatcggggc
     7861 gagccccgtc aggaagcccg caacgagcgc ggccgcgccg gcgaacagga gcgtgcgcgg
     7921 atcggttgcc acggcggacc ccgtggtgtc ccgcagcagc gcggcccgca gcacggctcc
     7981 gccccactgg gccaccagca cgccggccgt gccgccgagc agggccagca gcaggctctc
     8041 cgtgagcagc tggctcacga gccgggcgcg gctcacgccc atcgccaacc ggacggcgat
     8101 ctcgcgccgg cgccgcagcg cgcgcgccag cagcagattc gcgacattgg cgcaggcgat
     8161 caaccacacc accagggcga cgcccgtgat ccaggtcgcg accttggtct cgctcgactg
     8221 gttggggccg cgctccgaca gcaccgacgc cgcgatcgcg tgggggcgcg cgagggcgat
     8281 cggcgtcatc ccgccgtgat cggcggcgtc ctgcgtcatg tagctcttcc gataggcggt
     8341 ggtgaggtcg gcgttcgcgg cctcgagggt caccccgggc ttgcgctgca ccagcatgga
     8401 ggcccaggtc cagtggtagg tggtccacca ggtctcgccg cgcagacgga ggttcgcggc
     8461 cagctcgccg ccatacgtgg tgacggggat gtacgccgcc ggcggccgcc ccgaccacag
     8521 gcctacgaag tcgcgcgggg cgaccccgat cacggtgtag agcgtggcgc cgatgcgcag
     8581 cgtctggccg aggacgtccc ggcgcccacc gtagcgggtc tgccagaacc cgtagcccag
     8641 cacggtcacg gcggcgccgt tgggcggcga atcctccgtg gtcgtgaaat agcggcccag
     8701 cacgggcggg gcgtcgaaga agccgaagaa cccggcgctc acgaccccca cctgcatttc
     8761 gcgcgaatcg gtcccttccc cgatcgcgag ctgccgatcg gcgaacgtgg cggtgcgggc
     8821 gaacgaccgg gtgtccctgg tgagatcgag gtagcgcgca tattgcacgc cgtcgccggt
     8881 gaactcctcg ccccggtagg agcgggcgag atagacgcgg tgcacgagcg ccggatcacg
     8941 cagcagggga ggcggccgga acagcagccg gtccaccacg gagaacatcg ccgtgttggc
     9001 cccgatgccc agggcgagcg tcaggacgac ggcgacggtg aaccccggcc gggacttcag
     9061 gccgcggacg gcgtagcgga gatcctcgag ggccacgatt ctatgacgta ctgccccgga
     9121 agaaggttgc gctaggtggg agccacctga tactgctcca gcttcccgtc gggcagctcg
     9181 aacgtccaga cccgcagcga gcggtcggtg aagctcgcgc ggtaggaccg gtagaccatc
     9241 ccgccccgcc gctgctgggc cgtctggacg aacccctgaa gggcgcccag gggcccgagg
     9301 ctggacgcga aatcggcgac cgcctgggcg ctgaaatagg cgttgccgtc ggcggtgaag
     9361 agcgtgcggt cgatggtgcc atgctgcaac ccctcgaaaa tccgccgcgc ccgtgccgtc
     9421 cgtgatgcgg tcagggtgtc tccggtagcg aagagcagcc cggcgatgcg ctgggcgatg
     9481 gcgcccgagg tgctcaccgc gtcctgattg accagcacga tgacggcggc gtgctcgtcc
     9541 gggaacacca ggttcgtcgc cgtgaacccg gacacttccc caccgtggga gatcacgcga
     9601 tgtcccgcgt tcatccccac gtccacgccc agcccgtacc cggtcgcggt gctgttcttc
     9661 aacagggtct cggtctccag ctcgcggtag gacgcagggg acaacaggct ctcgttcatc
     9721 aatgtgatgt cccatttggc caggtcggac gccgtcattg ccagctcccc ggccgcgaac
     9781 agccagccct ttccttcttt cggcgccgca cgcggggggc ccaatgcgaa gcgctggtat
     9841 cccgtgggat ccgattgcgg caggcggccg aggtcgatgt tgaccacgct ctgaagcccg
     9901 agcggcgcca ggatgcgggt gcggagaaac tcgaagaacg gcatcccgct cgccttctcg
     9961 acgatcagcc ccgcggccac gtagttcgtg ttgctgtact gccactgggt cccgggctcg
    10021 aagtccagcg gcttcctggc ccagcgctcg aggatcccct gcgccgtcac cgagtcgagc
    10081 atgaagagcg gcacgtagtc ttggggccag tagtcctgat agcccgaggt atgcgacagc
    10141 agctggcgga tcgtgacgtc gcgcgcccgc gtcaggtcgg ggaggaactt cccgaccggg
    10201 tcgtcgaggg agagcttccc ctcctgctgg agcaacagga tcgcagtcgc gcagaactgc
    10261 ttgctgatgg acccgatgct gtagcgcatc tccgcacgcg cgaccacggg gggctcgaca
    10321 cgcgccgagc cgtacgcctg ggcgtagacc agccgcccct cctgcacaac ggccaccgac
    10381 gcactcggcg caccgctggc cttcagcacc tggcgggcga tcgagtccac catgggcccg
    10441 accggctgtt gcgactggag cggaacggtc cggccggcaa ggacgatccc tacccacacg
    10501 agtgccttcc tcataatcgg ctcctgatga agagagtccg tgccgctcga gcgaaagtct
    10561 cctatcttgc gacctatgac caaattgacc gttcgcctcg cgctgggcgc cgcgctcctc
    10621 gccccaaacg ccgctcccgc ccagcaaccc gccgccggct ctccgcaaac gcccgccccg
    10681 atccgagcgg gcgtcccgga tacgtccccg ttccgtatgc tgccgctgcc gccgcccgac
    10741 gtctaccggg cggcctccgg catgccggga ccctcatact ggcagcagcg ggccgattac
    10801 accatccgcg cctcgctcga taccgcgacc cacacgatcc ggggcgagga gacgatccgc
    10861 tacaccaaca actcgcccga cacgctgcgc ttcgtgtggc tgcagctcga catgaacgcc
    10921 ggctcgcccg acacgcgctt caccgccctc gcgaatccgt tcgcccgcac cgagcccggc
    10981 ttccgtgccg gagcgacgat cgagcgcgtg gcggcgctgc actcggccag cccccgcgct
    11041 tccccggtgg ccactcccct cacctggcgc gtcaacagca ccatgatgcg cgtcgatctc
    11101 gaccggccgc tgccgccgca cggcacggtg tcgctggccg tcgcctggca tcaccagatc
    11161 ccccgcttcg gccgcaccgg gcggcaacag ttcccgcagg gctggttgta tcaggtggcc
    11221 cagtggtatc cacgcctggc ggtgtacgac gacgtacgcg gctggaacac cgatcagtat
    11281 atcggctcga gcgagttcta cctcgagta
//
LOCUS       DBNGZW010000166         2219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1857703, whole genome shotgun sequence.
ACCESSION   DBNGZW010000166 DBNGZW010000000
VERSION     DBNGZW010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1857703"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="AC16JC_04110"
     CDS             <1..271
                     /locus_tag="AC16JC_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163914.1"
                     /translation="AYYYQGDPQRAAAVLEQLSAEAARRGDLAVQALALFNAAWLNGE
                     GGRGRVAATELADLNRLLRSPYMPDAVRDHLTARLAPTGAVAVTR"
     gene            complement(353..1138)
                     /locus_tag="AC16JC_04115"
     CDS             complement(353..1138)
                     /locus_tag="AC16JC_04115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5163915.1"
                     /translation="MRRFHLFEIEDQPWCPEFLRTAITDFLRALMEWFAPYAVAAPLL
                     ARALVQTGDTEILDLCSGGGGPWRDLLGRIPAAGGPAVRVRLCDLYPNRAAYARLGRL
                     SGGRIAAEPDPIDATAVPARLTGFRTLFTALHHFPPDAARAILADAVRQGRGIAVFEI
                     TRRSLLGLLGMLFLPVLALVLTPFIRPFRWDRLLWTYLVPVVPLVVWFEGTVSCLRTY
                     TPAELRRLVDGFDEYRWEIGTVRGPPLITLVTYLIGTPGGGRD"
     gene            complement(1148..>2219)
                     /locus_tag="AC16JC_04120"
     CDS             complement(1148..>2219)
                     /locus_tag="AC16JC_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163916.1"
                     /translation="IHCMSATRRVLLICGLALACSDSTAPARAPRTFRMGFSAIPPRA
                     DQTVTLQSLNLWTSGHADAAIMHISVPYLALLSGLTALQEVRGNEWGLANYYRAKGLR
                     LAVTVDVTNGLDRAKEDPVLDSLGRSITDTAIQRLYVEYVLALDSILHPDYLGLAAET
                     NLIRLAAPDSVYQAIVTMTNTVATRIQSQGSAAQLYVSVQVETAWGRFQGGGYAGIGQ
                     DLADFPFIDCLGLSSYPYLGGFADPESVPPDYYARLRTESARPVLVVEGGWTSTSLGA
                     LASSPAEQARYLVTQGRLLDSARAIAVFQLTFTDLDLSALNLPPGSGLPLFAHLGVVD
                     SALHAKPALAAWDEVFARPFAP"
BASE COUNT          384 a          764 c          793 g          278 t
ORIGIN      
        1 cgcctactac taccagggcg atccgcagcg tgccgccgca gtgctcgagc agttgtcggc
       61 cgaggcggcg cggcgcggtg acctggccgt gcaggcgctc gccctgttca acgccgcgtg
      121 gctgaacggc gaaggcggcc gcggccgggt ggctgcgacg gagctggccg acctcaaccg
      181 gctgctgcgc tctccgtaca tgccggacgc ggtccgcgac catctcaccg cgcgattggc
      241 cccgaccggt gcggtcgcgg tcactcgctg acgatcgccg gagtggaagg aggagcggat
      301 cgcgtgcggg gtcacgcgac ccgctccagc cgcacccgct gccgacagcc gctcagtcgc
      361 gcccccctcc cggcgtcccg atgaggtaag tcaccagggt gatgagcggc ggaccccgaa
      421 ccgtcccgat ctcccagcgg tactcgtcga acccgtccac cagccgccgt agctcggcgg
      481 gtgtgtaggt ccgcagacac gacacggtcc cctcgaacca cacgaccagc ggcaccaccg
      541 gcacgaggta ggtccagagc agccggtccc agcgaaacgg ccggatgaaa ggcgtcagca
      601 cgagggcgag cacgggcagg aacagcatgc cgagcagccc gagcagcgag cggcgggtga
      661 tctcgaacac ggcgatgccc cgcccctgac gcaccgcgtc ggcgaggatc gcgcgggcgg
      721 cgtcgggcgg gaaatggtgc agcgccgtga acagcgtgcg aaacccggtg aggcgcgcgg
      781 gcaccgcggt ggcgtcgatc gggtccggct cggccgcgat gcgaccgccg ctcagccggc
      841 cgagccgcgc gtaggccgcg cggttcgggt acagatcgca gaggcgcacg cggacggccg
      901 gcccgcccgc ggcggggatc cgcccgagca agtcgcgcca cggcccgccg ccccccgagc
      961 ataggtccag gatctccgtg tccccggtct gcacgagggc gcgcgccagc agcggcgccg
     1021 ccacggcgta gggcgcgaac cactccatca gcgcacgcag gaagtcggtg atcgcggtcc
     1081 ggaggaactc cgggcaccac ggctggtctt cgatctcgaa caggtgaaag cgtcgcaacg
     1141 aacgccctta tggagcgaac ggacgcgcga agacctcgtc ccaggcagcg agcgccggct
     1201 tcgcgtgcaa cgccgaatcc acgacgccca ggtgggcgaa gagcggcagt cccgaacccg
     1261 ggggaaggtt cagcgccgag aggtcgaggt ccgtgaaggt cagctggaag acggcgatcg
     1321 cgcgcgcgga atcgagcagc cggccctggg tcaccagata gcgcgcctgc tccgccgggc
     1381 tggaggcgag cgcgccgagc gaggtcgatg tccacccacc ctccaccacg agcaccgggc
     1441 gagcgctctc ggtccgcagg cgcgcgtagt agtcgggcgg cacgctctcc gggtcggcga
     1501 agccgccgag gtaaggatag gacgagagtc ccagacagtc gatgaaggga aagtccgcga
     1561 ggtcctggcc gatccccgcg taccccccgc cctggaagcg gccccacgcc gtctcgacct
     1621 gcacgctcac atacagttgc gcggccgatc cctgggactg gatcctcgtc gccaccgtgt
     1681 tggtcatcgt cacgatggcc tgatagaccg aatccggcgc cgcgagccgg atgagattgg
     1741 tttcggccgc gagtccgagg tagtcgggat gcagaatcga gtcgagcgca aggacgtact
     1801 cgacgtacag acgctgaatc gcggtgtcgg tgatgctccg gcccagcgag tcgagcaccg
     1861 ggtcttcttt ggcgcgatcg aggccgttgg tgacgtccac cgtgacggcg agccgcagac
     1921 ccttcgcccg atagtagttc gccagtcccc actcattgcc ccggacctcc tgcagcgcgg
     1981 tcaggcccga caacagcgcc aggtacggta ccgagatgtg catgatcgcc gcgtcggcgt
     2041 ggccggaagt ccacagattg agggattgca gcgtgacggt ctgatcggca cgcggcggaa
     2101 tcgcggagaa ccccatccgg aaggtgcggg gcgcgcgagc cggagcggtc gagtcgctgc
     2161 aagcgagggc gagcccgcaa atcagcagca cgcggcgtgt agcactcatg cagtgaata
//
LOCUS       DBNGZW010000167         4298 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_833423, whole genome shotgun sequence.
ACCESSION   DBNGZW010000167 DBNGZW010000000
VERSION     DBNGZW010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4298)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4298)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4298
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_833423"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="AC16JC_04125"
     CDS             complement(<1..286)
                     /locus_tag="AC16JC_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 4 glycosyl hydrolase"
                     /protein_id="HIR5163917.1"
                     /translation="MGLKVTVVGGGSTYTPELIEGFVNRAARLPIDQLVLLDIDLDRL
                     DVVGGLAQRMLDRLDWVGRLTLTGDRDSAIDGADFVLIQLRVGGQATRLVD"
     gene            394..1215
                     /locus_tag="AC16JC_04130"
     CDS             394..1215
                     /locus_tag="AC16JC_04130"
                     /inference="COORDINATES: protein motif:HMM:NF019322.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5163918.1"
                     /translation="MARFRLELGPIPLHHQVYLDLKAALDAAEWEPGDRLPTERDLAA
                     RYGCSLITIRRALGELVREGRLERTRGRGTFVLHPRIDRDFAGHLSFTEEMQTRGLDP
                     ETRLVAARPESAGEAVAAALDIELGSPTLYLERLRLADGQPLLLEQVHLPAERFPGLL
                     ASDLERGSLYDLLTEGYGVRVAKAREVLEPVLLRTREARLLGLRRGSPALLIEGIATS
                     ADGRPVEFGRTYVRGDRTRYYVERVVIRPHWQRASEVASQEEESNRAIAGQQLTR"
     gene            1235..2725
                     /locus_tag="AC16JC_04135"
     CDS             1235..2725
                     /locus_tag="AC16JC_04135"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5163919.1"
                     /translation="MERTVGRLGVLIAVAGLIFAACGSSTTSSAPSAVASAAPSAEAS
                     ASTAASGEASAAPSAITAGSTGKPGDVNVVWFCCLGTGDAPEQVTVEEQVAEAFNAAN
                     PGIHLSFQAYPYQGARDALSVQIGSGNGPDIVGPIGIGGANAFHGQWLDLQPLIDKNH
                     FDMSQFPSSTVSLYNVGGEGQVGIPYAIYPSVLFYKASLFKEAGLAEPPHQWNSTYTM
                     PDGSKVPWDYDTVRKLALLLTVDKNGKDATEAGFDPENIVQWGFEPQRDDMRQTGAYW
                     KAGSFVAADGKTVQMPDAWVAAWHYFYDAIWKDHTAVTGPQFVNTDFNPNGYPFFTGK
                     VAMSENYLWALYGVSDAGDDWNMAATPSYQGQTTAAFNADTFRILKGSKHPDEAFKVL
                     TYLLANQDLLKLYGGMPAVESKQDAFLQGLQADYKQTIDWKVAKEGVQFADVPNFESY
                     TPAYNQTLNLINTFYTKWQATPGLDIDKEVADMKTQMHAIWDQGGN"
     gene            2731..3678
                     /locus_tag="AC16JC_04140"
     CDS             2731..3678
                     /locus_tag="AC16JC_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5163920.1"
                     /translation="MIASGVARPRGRPRFSGLAWRRARWGYVFIAPWIIGFLAFTAFP
                     MVATLVFTFTNINLNQAEPLQFVGLRNYQTLVSDQRTWDSLAFTFKFALLALPVGVCL
                     PFVVALMLHSRHLRGSGAFRVLFFLPYVVPFVAGVFVWQGMLSTDTGWVNGLLKFIGI
                     SHPPDWLQDPAWIYPGLVIMGLWGIGGGMIIYLAGLKGIPSDLYEAAKIDGAGAFATL
                     RHITIPMMSPVIFFTIVLGVVDVLQYFLVPLVLKNGTGEPAGSTNFYNLYLYQNFFTF
                     QNMSYGATLAWLLFAISLALTLLLFRSARHWVYYAGEAR"
     gene            3682..>4298
                     /locus_tag="AC16JC_04145"
     CDS             3682..>4298
                     /locus_tag="AC16JC_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease family protein"
                     /protein_id="HIR5163921.1"
                     /translation="MAGVSAADLEDLTARPRLFGRRRAQPVGSRPPRERGDRTRSFVL
                     TLIAIVALALFLSPMLRSVVYAVKSTDQITQAGTPLWPADPVTFSYQGKELPLLLVPL
                     PDGSTRQLALFEPGRKSSTFVDPAAPDAAPITWQGSWRTLDQIWQFAPHWENFARVWD
                     LIDYPRLLFNTVAIAVISTIGTLLSCTLVAYGFARFKFPGRSVLFT"
BASE COUNT          669 a         1541 c         1397 g          691 t
ORIGIN      
        1 cgtcgaccag ccgggtcgcc tggccgccga cgcgcagctg gatcagcacg aaatcggcgc
       61 catcgatggc gctgtcgcgg tcgccggtca gggtcagccg tccgacccag tcgaggcggt
      121 cgagcatgcg ctgggccagt ccgccgacca cgtcgagccg gtcgagatcg atgtcgagga
      181 ggaccagctg gtcgatcggg agccgtgccg cgcggttgac gaagccctcg atgagctccg
      241 gcgtataggt gctgccgccg ccgacgacgg tgaccttgag gcccacggtc cactccagga
      301 ggggcttgac aggattgctt ataacgttat cagcaatcgc ggttcgcgca agccccggaa
      361 tagtcgaccc tcgaccgagg tccaagcccg ccgatggccc gcttccgact cgagctcggc
      421 cccatcccgc tccaccacca ggtctacctg gacctgaagg ctgccctcga cgcggccgag
      481 tgggagcccg gcgaccggct gcccacggag cgcgacctcg cggctcgcta cggctgcagc
      541 ctgatcacga tccggcgcgc gctcggcgag ctggtccgcg agggacggct ggagcggacc
      601 cggggtcgcg gcacgttcgt cctccatccc cggatcgacc gggacttcgc cggccacctg
      661 tcgttcaccg aggagatgca gacccgcggc ctggatccgg agacccggct ggtggccgcg
      721 cggcccgaat cggcgggcga ggcggtcgcg gcggcgctgg acatcgaact gggttcaccg
      781 accctctacc tggagcggct gcgcctcgcc gacggccagc cgctcctgct cgaacaggtt
      841 cacctgcccg cggagcggtt ccccgggctg ctcgcctcgg atctcgagcg cggctcgctg
      901 tacgacctcc tgaccgaggg gtacggcgtc cgcgtcgcga aggcgcgcga ggtgctcgag
      961 ccggtcctgc tgcggacgcg cgaggcccgg ctcctgggcc tccggcgggg cagtccggcg
     1021 ctcctgatcg aagggatcgc caccagcgcc gatggccgcc ccgtggagtt cgggcggacc
     1081 tacgtccgcg gcgatcggac ccggtactac gtggaacggg tggtcatccg cccccattgg
     1141 cagcgtgcga gcgaggtcgc gtcgcaagag gaggagagca accgtgcaat cgctggccag
     1201 cagctcaccc ggtagacgga atcggaggca agggatggaa cgcaccgtcg gacgcctcgg
     1261 cgtcctcatc gccgtagcgg ggctgatctt cgccgcgtgc ggctcgtcca cgaccagctc
     1321 ggcgcccagc gcggtagcgt cggccgcccc gagcgccgag gcctcggcat cgaccgccgc
     1381 gagcggcgag gcgtccgcgg cgccgagcgc gatcaccgcc ggctcgaccg gcaagcccgg
     1441 cgacgtcaac gtcgtctggt tctgctgcct cggcaccggc gacgcgccgg aacaggtcac
     1501 ggtggaggag caggtcgccg aggcgttcaa cgcggccaac ccgggcatcc acctgagctt
     1561 ccaggcctat ccgtatcagg gcgcccggga cgccctgtcg gtgcagatcg gctcgggcaa
     1621 tggacccgac atcgtcggcc cgatcgggat cggcggcgcc aacgccttcc acgggcagtg
     1681 gctcgacctg cagccgctca tcgacaagaa ccacttcgac atgagccagt tcccgtcctc
     1741 gacggtgagc ctgtacaacg tcggcggcga gggccaggtc gggatcccgt acgcgatcta
     1801 tccatcggtc ctgttctaca aggcgagcct gttcaaggag gccggcctcg ccgagccgcc
     1861 gcaccagtgg aacagcacct acaccatgcc cgacggatcc aaggttccgt gggactacga
     1921 caccgtccgc aagctcgcgc tcctgctgac cgtcgacaag aacgggaagg acgcgaccga
     1981 ggctgggttc gacccggaga acatcgtcca gtggggcttc gagccccagc gtgacgacat
     2041 gcgccagacc ggcgcctact ggaaggccgg ctcgttcgtc gccgcggacg gcaagaccgt
     2101 ccagatgccc gacgcgtggg tggccgcgtg gcattacttc tacgacgcga tctggaagga
     2161 ccacaccgcg gtcaccggtc cgcagttcgt caacaccgac ttcaacccca acggctaccc
     2221 gttcttcacc ggcaaggtgg ccatgagcga gaactacctc tgggcgctgt acggcgtgtc
     2281 cgatgcgggc gatgactgga acatggccgc gaccccgtcc taccagggcc agaccacggc
     2341 cgccttcaac gccgacacct tccgcatcct caagggcagc aagcaccccg acgaggcgtt
     2401 caaggtgctc acctacctgc tggccaacca ggacctgctc aagctgtacg gcggcatgcc
     2461 cgcggtcgag tccaagcagg atgcgttcct ccagggcctc caggcggact acaagcagac
     2521 gatcgactgg aaggtggcca aggaaggcgt ccagttcgcg gacgtgccca acttcgagtc
     2581 gtacaccccc gcctacaacc agaccctcaa cctgatcaac accttctaca cgaagtggca
     2641 ggcgacgccc ggcctggaca tcgacaagga ggtcgccgac atgaagaccc agatgcatgc
     2701 gatctgggac cagggcggca actgacctag atgatcgcct ccggcgtggc gcgcccgcgc
     2761 ggccggcccc ggttctccgg tctggcctgg cggcgcgccc gctggggcta cgtcttcatc
     2821 gccccgtgga tcatcggttt cctcgcgttc accgccttcc cgatggtggc cacgctggtc
     2881 ttcaccttca ccaacatcaa cctcaatcag gccgagccgc tccagttcgt cggcctgcgc
     2941 aactaccaga cgctggtgtc ggaccagcgg acctgggatt cactggcgtt caccttcaag
     3001 ttcgcgctcc tggcgctccc ggtcggcgtg tgcctcccgt tcgtggtcgc gttgatgctg
     3061 cactcgcgcc acctgcgcgg gtccggcgcg ttccgcgtcc tgttcttctt gccgtacgtg
     3121 gtgccgttcg tggccggcgt cttcgtctgg cagggcatgc tcagcaccga tacgggctgg
     3181 gtcaacgggc tgctcaagtt catcgggatc agccacccgc cggattggct ccaggacccc
     3241 gcctggatct accccgggct cgtgatcatg ggcctgtggg ggatcggcgg cgggatgatc
     3301 atctacctcg ccgggctcaa gggcatcccg tccgacctgt acgaggcggc caagatcgac
     3361 ggtgccggcg ccttcgcgac gctgcgccac atcacgatcc cgatgatgtc gccggtgatc
     3421 ttcttcacca tcgtcctcgg cgtcgtcgac gtcctccagt acttcctcgt cccgctggtc
     3481 ctcaagaacg gcaccggcga gcccgccggc tcgaccaact tctacaacct ctacctgtat
     3541 cagaacttct tcacgttcca gaacatgtcg tacggcgcca cgctggcctg gctgctgttc
     3601 gccatctcgc tcgcgctcac gctgctgctc ttccgcagcg ccaggcactg ggtctactac
     3661 gcgggcgagg cacgctgaac gatggccggc gtcagcgcgg cggatctcga ggacctgacc
     3721 gcccgcccgc ggctgttcgg ccggcggcgt gcgcagccgg tggggagccg gccgcctcgc
     3781 gagcgtggcg accggacgcg ctcgttcgtc ctgaccctga tcgcgatcgt tgccctggcc
     3841 ctgttcctgt cgcccatgct tcgctcggtc gtgtacgcgg tcaagagcac ggaccagatc
     3901 acgcaggccg gcacgccgct ctggccggcc gatccggtga cgttctcgta ccagggcaag
     3961 gaactgccgc tgctgctcgt gccgttgccg gatggcagca cccgccagct cgcgctgttc
     4021 gagccggggc gcaagtccag cacgttcgtc gacccggccg cgcccgatgc ggcgccgatc
     4081 acctggcagg gatcgtggcg gacgctcgac caaatctggc aattcgcccc gcactgggag
     4141 aacttcgcca gggtctggga cttgatcgac tacccgcgac tgctgttcaa cacggtcgcc
     4201 atcgcggtca tctcgacgat cgggacgctg ctgtcgtgca ccctcgtcgc ctacggcttc
     4261 gcccgcttca agttcccggg gcgaagcgtc ctgttcac
//
LOCUS       DBNGZW010000168         2341 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1107434, whole genome shotgun sequence.
ACCESSION   DBNGZW010000168 DBNGZW010000000
VERSION     DBNGZW010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1107434"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="AC16JC_04150"
     CDS             <1..1079
                     /locus_tag="AC16JC_04150"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR5163922.1"
                     /translation="GAVSFIDKAEPMERVVQEIAGALERRRLEQEVEALRQDLGADTP
                     LVGSSPAMQKLKDALARIAPIPSPVLITGQSGSGKEVVARTVHRMSGAKGPFLAINCA
                     ALPADLVESELFGHERGAFTGAVAMRKGAFESAAGGTLFLDEVGELPLAAQAKLLRVL
                     EQREVTRVGGTRTIAVDARVLAATNRDLAAEVAAARFREDLLYRLNVHVLAVPSLRDR
                     LSDVPELVHHLLTSICARFRMRPKPLTAAALECLMGYDWARNNVRELRNVLERMLIAT
                     DAPEMGEDDVPPEIREHGAARRVSSGAEPRTFHELKAEAERQIIVAALERHDWHVTRT
                     AEALGLADHASLLKIMRRHGISRG"
     gene            1351..>2341
                     /locus_tag="AC16JC_04155"
     CDS             1351..>2341
                     /locus_tag="AC16JC_04155"
                     /inference="COORDINATES: protein motif:HMM:NF021492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5163923.1"
                     /translation="MHSAVLSLVPLLLYHRALTHPNRRAAPLMEEESMITTLRFTPAW
                     LAAGLFVLAGCGDKSPKQASAKEPGAPESTVSVASESTPAPAEPVTVSTAPVSFETAE
                     AAFRERRYADAVRDFTVYTADRPGNAWGFYMLGLSSWKSGDLQTAEEALRQALTIDPA
                     HVKSYLNLSRVLLDAGQPDSAIAMLDTVLKIDDSLGEAYRLIGRAHDAKGERDEAVTA
                     YHEALVLDSTDVWAMNNLGHSLIVQGSYDEALRPLARAVELVPEVATFQNNLGIALER
                     TGHFTLAAEAYRAALAADSGFTKASVNLQRVETLKEDPNIPPVDLKSLAQEFVQ"
BASE COUNT          387 a          778 c          828 g          348 t
ORIGIN      
        1 tgggcgcggt gagcttcatc gacaaggccg agccgatgga gcgggtggtg caggagatcg
       61 ccggcgcgct cgagcgccgt cgcctcgagc aggaagtcga ggcactgcgg caggatctcg
      121 gggccgacac gccgctcgtg ggctcgagcc cggcgatgca gaagctcaag gacgcgttgg
      181 cgcgcatcgc gccgattccc tcgcccgtcc tgatcacggg gcagagcggg tcgggaaagg
      241 aagtcgtggc ccgcacggtg caccgcatga gcggcgcgaa ggggccgttc ctcgccatta
      301 actgcgcggc actgccggcg gacctggtgg agagcgagct gttcggccac gagcgcgggg
      361 ctttcactgg cgcggtcgcc atgcgcaagg gcgcgttcga atcggccgcg ggcggcacgc
      421 tgttcctgga cgaagtgggc gagctgccgc tcgcggccca ggccaagctg ctgcgtgtgc
      481 tggagcagcg cgaggtgacg cgcgtcgggg ggacgcggac gatcgcggtg gatgcgcggg
      541 tgctcgccgc caccaaccgc gatctcgcgg cggaggtcgc cgcggcacgg tttcgcgagg
      601 atctgctcta ccggctcaac gtccacgtgc tcgcggtgcc gagcctgcgc gaccggctgt
      661 ccgacgtgcc cgagctggtc catcacctgc tgacttcgat ctgcgccaga ttccgcatgc
      721 gcccgaagcc gctcacggcg gcggcgctcg aatgcctgat gggctacgac tgggcgcgga
      781 acaacgtacg ggagctgcgc aacgtgctgg agcggatgct catcgcgacg gacgctccgg
      841 agatggggga ggacgacgtc ccgccggaaa tccgcgagca cggtgcggcg cgccgggtga
      901 gcagcggcgc cgagccgcgc acgttccacg agctcaaggc cgaagcggag cggcagatca
      961 tcgtggcggc gctcgagcgg cacgactggc acgtcacgcg caccgccgag gcactcggcc
     1021 tcgccgacca cgcgagcctg ctgaagatca tgcggaggca cggaatcagc agagggtagg
     1081 agcgcggtac ggtcgggcgc ccgctcgttc tcgcgagtaa actcgcgact cggctgaact
     1141 atcgcttgag agcgactcgt cacttcacgt gacagatagc cgagtcgcgg ctctcagccg
     1201 cgaaaaacaa cgcgcgtcgc tctccgccat cctccacgtg tcctcccgga cacgacccac
     1261 cctcgtttga cgtgtccatt gggtcacaat cgcccctgcc cttgatttcg cccgctcgga
     1321 caaccgcctc cgctccaatc atttgcatcc atgcattcgg ccgtgctcag cctggtcccc
     1381 ctcctgcttt accacagggc gctgacacac cccaaccggc gcgcggcgcc cctgatggag
     1441 gaggaatcga tgatcaccac cctgcggttc accccggcct ggctcgcagc cggactgttc
     1501 gtgctggccg gctgcggcga caagtctccc aagcaggcga gcgcaaaaga gccgggcgct
     1561 ccggagtcca cggtgagcgt ggcatcggaa tcgacgcccg caccggccga gccggtaacc
     1621 gtgagcacgg cccccgtgtc gttcgagacc gcggaagcgg cgttccgcga gcggcgctac
     1681 gccgacgcgg tgcgcgattt cacggtgtat accgccgacc gtcctgggaa cgcgtggggc
     1741 ttctacatgc tcgggctgtc gtcctggaag tccggggatc tccagacggc cgaggaagcg
     1801 ttgcggcagg cgctgacgat cgacccggcg cacgtgaaga gctacctcaa cctgagccgg
     1861 gtgctgctcg atgcagggca gccggattcc gccatcgcga tgctcgacac cgtgctgaaa
     1921 atcgacgaca gtctgggtga agcctaccgg ctgatcgggc gggcgcacga tgcgaagggc
     1981 gagcgcgacg aggcggtgac cgcctatcac gaggcgctgg tgctcgattc gaccgacgtg
     2041 tgggcgatga acaacctcgg gcacagcctc atcgtgcagg gctcgtatga cgaggcgcta
     2101 cgcccgctgg cccgtgcagt cgagctggtc cccgaggtgg cgacgttcca gaacaacctc
     2161 ggcatcgcgc tggagcggac cgggcatttc acgctggcgg ccgaggcgta ccgcgcggcg
     2221 ctggcggcgg actcggggtt caccaaggct tcggtgaatc tgcagcgggt cgaaacgctc
     2281 aaagaggatc cgaacatccc gccggtggac ctgaagtcgc tggcgcagga gttcgtgcag
     2341 c
//
LOCUS       DBNGZW010000169         3955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_16042, whole genome shotgun sequence.
ACCESSION   DBNGZW010000169 DBNGZW010000000
VERSION     DBNGZW010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_16042"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..539)
                     /locus_tag="AC16JC_04160"
     CDS             complement(3..539)
                     /locus_tag="AC16JC_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HIR5163924.1"
                     /translation="MRGTVVKKGRRHAATGLYAHITWHTWRRQFAVYEPDVPVIIAAI
                     RAAAERTGFHIAGVAVLAEHVHVVVSYLPTANVSSFIREAKSESARRVRLARPDSRLR
                     WGRGYHAGSLSWSHVRAARCYVGRQRRRHPDRLPARVDFIPGSAARGPSGWTPGPCPG
                     VPGCLSPRVGSPRGHAPG"
     gene            complement(656..1816)
                     /locus_tag="AC16JC_04165"
     CDS             complement(656..1816)
                     /locus_tag="AC16JC_04165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HIR5163925.1"
                     /translation="MGALSHDLYQARTVLAERFGYPAFRAAQLRVLAPLLGGRHVLAV
                     LPTGAGKSLCYQVPALLASGLTIVVSPLISLMQDQVATLRRRGIPAAFLNSTLSAADQ
                     RTILDLALRGRARLFYCAPERLASLARLLREAGTRVSLLAVDEAHCIVEWGSTFRPTY
                     QRLGELRYSLREPQTLALTGSATPAARLEILRVLRIPRAEVVTTTFDRPNLRFEVVRV
                     KDDRDRFARLTEAVGQAAEQVIVYAPTRRLTELVTRALRFRGLVAAPYHAGLDSDIRK
                     PILGAFLKDKAPVIVATSAFGMGIDKPDVRLVLHWGPPRTLESYYQEAGRAGRDGRPA
                     RCRILWRPDDFRYGEVTPGMRTYVERRRCRRRMLLEHFGERFAACSGCDVCG"
     gene            complement(1880..2284)
                     /locus_tag="AC16JC_04170"
     CDS             complement(1880..2284)
                     /locus_tag="AC16JC_04170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012925684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR5163926.1"
                     /translation="MTDSAEQPAAPDESPKRLVVVGDRVLIVPEEGEERTNVGLYLPQ
                     TALDSRQVQGGRIVATGPGTPMTEPTSMDDEPWKIRGPDVKYLPMQAEVGDYALFFRK
                     AAVELTFEGKTYLVVPQAAILVLVQREVPEPE"
     gene            complement(2268..2702)
                     /locus_tag="AC16JC_04175"
     CDS             complement(2268..2702)
                     /locus_tag="AC16JC_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HIR5163927.1"
                     /translation="MAGLNTFMYDPRMVQPMREELTSIGFEELRTSEEVDRVLGTEKA
                     TTLVVVNSVCGCAARNARPAAALALQHPKRPARLLTVFAGQDADATARARGYFTGVAP
                     SSPQMGLLKDGKLVFMLERRNIEGRPAADIAADLVAAFDRFC"
     gene            complement(2749..>3955)
                     /locus_tag="AC16JC_04180"
     CDS             complement(2749..>3955)
                     /locus_tag="AC16JC_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0016600 - flotillin complex [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HIR5163928.1"
                     /translation="RVGGRMLTAAWLFGAVFGILVLAGLAAYFLFRDVPAGQIRLVNW
                     LQGASRIYRGPTKSKELPLLATSTTVSNRPVSLELDVTEQTADTDGAGRPRPIEVRVR
                     ATAIVGVGDSDAMIRLAANRFCPMSETDQTRTLADLLTSGTRESLTRLRHDALFFVGT
                     TAGEGGAAITEHPLAALTRDICASRLADLGLALRAFSIKAVHSEVAEARRREAAAEAK
                     AESLIASVQHDRRAREAQLEADRVISDKERELEQVRAQNSGLIAQATARQQEIKAKAD
                     AERVRIEAEAAKEALRGAQFGLALDEALRITKIAAAQAEGFRKVNDTIREGGDSYFRY
                     KLIEMLPQLTPAIAEALSKASSSGESLPASPSGKEVVTTGGNGPTRKRSRPPFRDRAE
                     GAAAPEPDA"
BASE COUNT          619 a         1419 c         1316 g          601 t
ORIGIN      
        1 gttcaccccg gggcgtggcc ccggggtgaa cccacccggg gactcaggca cccggggacc
       61 ccggggcacg gccccggggt ccacccactc ggacctcggg cagccgatcc cggaataaaa
      121 tccacacgag cggggagccg atccggatgt cgccttcgtt gacgccctac gtagcaccgt
      181 gccgctcgca cgtggctcca gctgagggag cccgcatggt agccgcggcc ccagcggagg
      241 cgagaatcgg ggcgtgcgag tctgacgcgc cgggccgatt ccgacttcgc ttcacgaatg
      301 aaactgctca cgttggccgt gggcaggtag ctgacgacca cgtggacgtg ctccgcaagc
      361 accgccacgc cggcgatgtg gaacccagtt ctctccgcgg cagcccgaat ggcggcgata
      421 atgaccggca cgtctggctc gtagacggcg aactgccgcc gccaggtatg ccaagtgatg
      481 tgcgcataca acccggtggc cgcatgtctc ctccccttct tcacgacggt tcctctcacg
      541 ggtgggttta cgccggggcc acgccccggc gttcagggaa cgccgcgccc accgcatgag
      601 tgggtgaaac ccgaggccgg cgggtggatt gacccagggg gtgcgcggag ccagcctacc
      661 cgcacacgtc acacccgctg cacgccgcga accgctcccc aaagtgctcc agcagcatgc
      721 gccggcggca gcggcggcgc tccacgtacg tacgcatccc cggcgtgacc tcgccgtaac
      781 ggaaatcatc cggccgccac aggatccggc agcgcgccgg ccgcccgtcc cggcccgccc
      841 gccccgcctc ctgatagtag gactcgagcg tgcgaggagg gccccaatgc aacaccagtc
      901 gtacgtcggg cttgtcgatc cccatcccga aggcgctggt cgccacgatc accggcgcct
      961 tgtctttgag gaacgccccg aggatcggct tgcggatgtc gctgtccagt ccggcgtggt
     1021 agggcgccgc gaccagcccg cgaaaccgca gcgcgcgcgt gacgagctcc gtgagcctcc
     1081 gggtcggggc atagacgatg acctgctccg ccgcctgtcc caccgcctcc gtcaggcgcg
     1141 cgaagcgatc gcggtcgtcc ttcacccgca cgacttcgaa gcgcaggttc ggccgatcga
     1201 acgtggtggt cacgacctcc gcccgcggga tgcggagcac acgaaggatt tcgagccggg
     1261 cggccggcgt cgcactgccg gtgagcgcca gggtctgggg ctcccgcagg gagtagcgca
     1321 gctcacccag acgctgatag gtgggacgga aggtgctccc ccattccacg atgcagtgcg
     1381 cctcgtcgac cgcgagcaac gacactcgag tcccggcctc tcggagcagc cgggcgagcg
     1441 aggcgagtcg ctccggggcg cagtaaaaca gccgggcccg tcccctcaac gcgagatcga
     1501 gaatggtccg ttgatcggcg gccgacaggg tgctgttgag aaaggcggcg gggatgccgc
     1561 gacgtcgcag tgtcgccacc tgatcctgca tcagcgagat caggggagag acgacgatcg
     1621 tcaacccgct cgccagcagg gccgggacct ggtagcacag cgatttgcct gcgccggtgg
     1681 gcagcacggc gaggacgtga cgtccaccca gcagcggcgc gagcacgcgc agctgcgccg
     1741 cgcggaacgc ggggtagccg aaacgttcgg ccaagaccgt cctggcctga tacaggtcgt
     1801 gcgagagggc gcccaccgct cccaaagaaa acgaccccgt ccaggaaacc ggggccgatt
     1861 tcacgcgagc gggtaccgat tattccggct cggggacctc gcgttgcacg aggacgagaa
     1921 tcgccgcctg cggcaccacc aggtaggtct tcccctcgaa cgtgagttcc accgccgcct
     1981 tgcggaagaa cagggcgtaa tcccccactt ccgcctgcat cggcaggtac ttcacatcgg
     2041 ggccgcggat cttccacggc tcgtcgtcca tgctggtggg ctcggtcatc ggcgtgcccg
     2101 gcccggtcgc cacgatccgg cccccctgca cctgccggga gtcgagcgcg gtctgcggca
     2161 gatagagccc gacgttggtg cgctcttcac cctcctccgg cacgatcagg acgcggtcgc
     2221 cgaccacgac cagccgcttg ggcgactcgt cgggcgcagc gggctgctca gcagaatcgg
     2281 tcaaacgccg ccaccaggtc cgcggcgata tcggccgcgg gccgcccctc gatgttccgc
     2341 cgctccagca tgaacacgag tttcccgtcc ttgagcagtc ccatctgggg agaggacggg
     2401 gccaccccgg tgaaatagcc gcgggcgcgg gcggtggcgt cggcatcctg gccggcgaac
     2461 acggtgagga ggcgcgcggg acgcttcgga tgctggagcg cgagcgccgc cgccggacgc
     2521 gcgttgcgcg ccgcgcaccc gcagaccgag ttgacgacga ctagcgtggt cgccttctcc
     2581 gtccccagca cgcggtccac ctcctcggag gtccgcagct cctcgaaccc gatgctggtc
     2641 agctcctcac gcatcggctg caccatgcgg gggtcgtaca tgaacgtatt gagaccagcc
     2701 atgtcatcct cgagtggaat cggctgatca actataacca gcgtggcgtc acgcgtcagg
     2761 ctcgggagcc gcggcgccct ccgcccggtc tctgaacggc gggcggcttc gcttgcgcgt
     2821 cggtccgttg cctccggtcg tcaccacctc cttgccggac ggcgacgccg gcagcgactc
     2881 tcccgatgag ctcgccttgg agagcgcttc ggcgatggca ggggtgagct gcggcagcat
     2941 ctcgatcagc ttgtagcgga agtagctgtc tcccccctcc cgaatggtgt cgttgacctt
     3001 gcggaatccc tcggcctgcg cggccgcgat cttggtgatg cgcagcgctt cgtccagggc
     3061 cagcccgaac tgggcacccc gcaacgcctc cttggcggcc tcggcctcga tccgcacgcg
     3121 ctccgcatcc gccttcgcct tgatctcctg ttgccgcgcc gtcgcctgag cgatcaaccc
     3181 cgagttctgc gcccgcacct gctcgagctc gcgctcctta tccgagatga cgcggtccgc
     3241 ttccagctgc gcctccctcg cgcggcggtc gtgctgcacc gaggcgatca gggattccgc
     3301 cttggcttcc gccgccgctt ctctgcgacg ggcctccgcg acctcggagt gcaccgcctt
     3361 gatgctgaac gcccgcaggg ccaggcccag gtcagccagc cgcgacgcgc agatgtcacg
     3421 cgtcaaggcc gcgagcgggt gctcggtgat cgccgcgccg ccttccccgg cggtcgtccc
     3481 gacgaagaac agcgcgtcgt gccgcaatcg ggtcaggctc tcgcgcgtgc cactcgtgag
     3541 caggtcggcc agggtgcggg tctgatcggt ctcggacatc gggcagaagc ggttggccgc
     3601 cagccggatc atggcgtcac tgtcgccgac tcccacgatg gcggtcgccc gcacgcgaac
     3661 ctcgatcggg cggggtctcc ccgctccgtc ggtatcggcg gtctgctcgg tgacgtccag
     3721 ctccaggctg accggtctgt tggagaccgt cgtggaggtc gcgagcaggg gcaactcttt
     3781 ggatttcgtg ggtccgcggt agatgcggct ggcaccctgc aaccagttca cgaggcggat
     3841 ctggcccgcc ggtacgtcgc ggaacaggaa gtaggcggcc aaccctgcca gcaccaggat
     3901 accgaacact gccccgaaca gccacgctgc cgtcagcata cgccctccaa ctcga
//
LOCUS       DBNGZW010000170         2664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_357935, whole genome shotgun sequence.
ACCESSION   DBNGZW010000170 DBNGZW010000000
VERSION     DBNGZW010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_357935"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1698
                     /locus_tag="AC16JC_04185"
     CDS             <1..1698
                     /locus_tag="AC16JC_04185"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5163929.1"
                     /translation="DEPAISPRGDRVAFLRGGQVWALPLDGSKPAAQLFFTRGANDGL
                     TWSPEGNRLAFVSRRGDHSFIGIFASDSEAIRYLAPSTTRDLDPHWSPDGVRIAFLRL
                     PGAGGSPESMLEVTPQPWAIWTADAATGGAREVWRSPNTLHGSRPTTAGGANLAWGAG
                     GDRLVFLADLDGWPHLYSIPANGGTPLLLTPGAFMVEHVAVTPDGHSIVYSANTGPDS
                     NDVDRRHLFTVAVDRPGPRPLTTGAGLEWTPVLTGDGQTVALISGEAKRPPVPAVLPA
                     GGGRLRLLGEDRIPADYPVAAMVVPRKVVFRAPDGTLIHGQLFRRVDLAGKHPGVVFV
                     HGGPPRQMLLGWHYMDYYSNAYAVNQYLASRGYVVFSVNYRLGIGYGHDFHHPPHAGA
                     SGASEYQDVLAGGRYLGGLAEVDARRIGIWGGSYGGFLTAMALARNSDVFATGVDLHG
                     VHDWASQARGRFSPPAWRYEQGDLDSARAVAWRSSPVSAIATWKSPVLLIQGDDDRNV
                     YFSEMVDLDDRLDAAGVPHEDLVIPDEIHGFLRHQSWVTADSATVAWFAKKLVIGDR"
     gene            complement(1763..>2664)
                     /locus_tag="AC16JC_04190"
     CDS             complement(1763..>2664)
                     /locus_tag="AC16JC_04190"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014501797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5163930.1"
                     /translation="PVTVLRDPTGRIVLTLERADISRLTATGWRPPLPITVKARDGKT
                     DLYGLLYRPTTFDSTRRYPIVDHIYPGPQTGSVGSRSFSAARGDAQALAELGFVVVEI
                     DAMGTPLRSKSFHAAYYGDMGDNGLPDQVAAMRELAQRYSWIDLDRAGIYGHSGGGYA
                     AADAMLRYPDFFKVGVSEAGNHDNREYEDDWGEKWQGLLVKDADGTTNYDNQANQLQG
                     EHLKGKLLIAYGTTDNNVPPDNSLLLVDELIKANKDFDLLALPNRRHGFGSEPYMIRR
                     RWDYFVRWLLDAEPPAGYRLRGK"
BASE COUNT          379 a          901 c          949 g          435 t
ORIGIN      
        1 gacgagcccg cgatctcgcc gcgcggcgac cgggtggcat tcctgcgcgg cggccaggtg
       61 tgggcgctgc cgctggacgg ctccaagccg gcggcgcagc tgttcttcac ccgcggcgcg
      121 aacgacggac tcacctggtc gccggagggg aaccggctcg ccttcgtgtc gcgccgcggc
      181 gaccacagct tcatcgggat cttcgcctcg gactcagagg cgatccggta tctcgcaccg
      241 tcgacgacgc gggatctcga tccgcactgg tccccggacg gcgtgcggat cgcattcctc
      301 cgtctgccgg gggcgggcgg gtcgccggag tcgatgctcg aggtgactcc gcagccgtgg
      361 gcgatctgga cggccgacgc tgccacggga ggcgcccgcg aagtctggcg cagcccgaac
      421 acgctgcacg ggtcgcggcc caccacggcg ggcggggcca atctcgcctg gggcgccggc
      481 ggcgaccgcc tcgtgtttct cgccgatctg gacggctggc cgcacctcta ttcgatcccg
      541 gcgaacgggg gaacgccgct gctgctgacg cccggcgcct tcatggtaga gcacgtcgcg
      601 gtgacgcccg acggccacag catcgtctac agcgccaata ccggaccgga cagcaacgac
      661 gtcgaccggc ggcacctctt caccgtcgcc gtggatcggc cggggccgag gccgctcact
      721 acgggggcgg gcctcgagtg gacgccggtc ctcaccggcg atggacagac cgtggcgctg
      781 atcagcggcg aggcgaagcg tccgccggtg cccgccgtct tgcccgcggg cggagggaga
      841 ctgcgcttgc tgggtgagga tcgcatcccg gcggattacc cggtggcggc gatggtcgta
      901 ccgcgcaagg tcgtgttccg cgcgccggac gggacgttga tccacgggca gttgttccgg
      961 cgggtggacc tcgccgggaa gcacccgggc gtcgtgttcg tgcacggcgg accgccgcgg
     1021 cagatgctgc tgggctggca ctacatggac tactactcga acgcctacgc ggtgaaccag
     1081 tatctggcga gtcgcggcta cgtcgtgttt tcggtgaact accgcctcgg gatcggctac
     1141 ggacacgact tccaccatcc gccgcacgcg ggcgcatcgg gcgcgagcga gtaccaggac
     1201 gtgctcgccg gcggccgcta tctcggcggg ctggcggaag tggacgcgcg ccggatcggg
     1261 atctggggcg gctcctacgg cggcttcctc accgccatgg cgctggcgcg caactccgac
     1321 gtgttcgcga ccggcgtcga cctgcacggg gtgcacgact gggcgtcgca ggcccgggga
     1381 cgattctcac cacccgcatg gcgctacgag cagggtgact tggacagcgc gcgggcggtg
     1441 gcgtggcggt cgtcgcccgt ctccgccatc gctacctgga agtcgcccgt gctcctcatc
     1501 cagggcgatg acgaccgcaa cgtctacttc tccgaaatgg tggacctcga tgaccggctc
     1561 gacgcagcgg gggtgcccca cgaggacctc gtgatccccg acgagatcca cggcttcctg
     1621 cggcaccagt cgtgggtcac ggcggattcg gcgacggtcg cctggtttgc gaaaaaactg
     1681 gtcatcggtg atcggtaatc ggtattcggt cctcggtagt cggtgagcga ccgacgaccg
     1741 atcaccgagg accgatcacg gttcacttcc cgcgcagccg gtatcccgcc ggcggctcgg
     1801 catcgagcag ccagcggacg aagtagtccc agcgccgccg gatcatgtac ggctcgctgc
     1861 cgaaaccgtg ccgccggttg gggagcgcca acagatcgaa gtccttgttc gccttgatca
     1921 gctcgtccac cagcagcagc gagttgtcgg gcgggacgtt gttgtcggtc gtgccgtagg
     1981 cgatcagcag ctttcccttc aggtgctcgc cctggagctg gttcgcctga ttgtcgtagt
     2041 tcgtagtgcc gtcggcgtcc ttcaccagga ggccctgcca cttctcgccc cagtcgtcct
     2101 cgtactcccg gttgtcgtgg ttaccggcct cggagacgcc caccttgaag aaatcggggt
     2161 agcgcagcat cgcgtcggcc gccgcgtagc cgccgcccga gtggccgtag atgcccgcgc
     2221 ggtcgagatc gatccaggaa tagcgctggg ccaactcgcg catcgcggcg acctgatcgg
     2281 ggagtccgtt gtctcccatg tcgccgtagt aggcggcgtg gaacgacttg gagcgcagcg
     2341 gcgtgcccat ggcgtcgatc tccaccacca cgaaccccag ctcggccagt gcctgagcgt
     2401 caccgcgggc cgctgagaag ctgcggctgc cgacgctccc cgtctgcggc cccggataga
     2461 tgtggtcgac gatcgggtac cgtcgcgttg agtcgaacgt cgtgggacgg tacagcaggc
     2521 cgtagaggtc ggtcttcccg tcgcgcgcct tcaccgtgat gggcagcggt ggccgccacc
     2581 ctgtggccgt gaggcgtgag atgtcggcgc gctcgagcgt gaggacgatg cggccggtcg
     2641 ggtcgcgcag cacggtgacc gggg
//
LOCUS       DBNGZW010000171         3086 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_971153, whole genome shotgun sequence.
ACCESSION   DBNGZW010000171 DBNGZW010000000
VERSION     DBNGZW010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_971153"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="AC16JC_04195"
     CDS             <1..320
                     /locus_tag="AC16JC_04195"
                     /inference="COORDINATES: protein motif:HMM:NF042466.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminomethyltransferase beta-barrel
                     domain-containing protein"
                     /protein_id="HIR5163931.1"
                     /translation="GYPEPMYVVDIRPDTREVVIGPGDALFGHAVTLTDVNWLAAPLM
                     AGDHVLVQCRYRARAVPGTIDSIRPDALALRLAEPVRAITAGQSGVLYDEAGRVLGGG
                     LIA"
     gene            448..1575
                     /locus_tag="AC16JC_04200"
     CDS             448..1575
                     /locus_tag="AC16JC_04200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163932.1"
                     /translation="MSVPRALRASVVVGIAFAAACSDSSHLGPGTLVDPTATAAALAS
                     FDSAFGSATLASFTSLSLFMAPTAALAPARRVLTATQPVPFAAGRSPEAALAGRLSGL
                     RDLASLTLNPQGPLIPDTLYGSIFTWDSAGAGYTRSQTTGGPGNGVRFILYAVNPLTG
                     AVSYPLTPVGQVDLLDESSGTSAQLHIIVAGNGGTPTYVDYTTTLTFGLGTLTATVTG
                     SVTNALQPPANKTLSFSAIASFSLTDISVHATYTLNNPAFTVVLDIVDANSQPTDTVD
                     VDLLISRPNEAVRFTAGLVTTGGVVDTVSAAITVNAQLYARMEGNATGVTFYDKNGAP
                     IQDTATQHDILVALDHIRHVAEGVLTFTAALFQPIINLLTG"
     gene            1670..2971
                     /locus_tag="AC16JC_04205"
     CDS             1670..2971
                     /locus_tag="AC16JC_04205"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR5163933.1"
                     /translation="MTSRRRFLRSMLSASAAVPVVPALTNDALTHIARTVGAVAGRPP
                     TALADDEDFWREIQNAFTLDRTLINLNNGGVSPSPRVVQEAMERYLAISNTAPVITMW
                     AWLEPEIESVRRRLAADFGCDPEEMAITRNASEALEIVQLGMTLQRGDEVLTTTQDYP
                     RMITTWKQRERRDGIVLKQITFPVPPPSTDDLVRRFAEAITPRTRVIHVCHITNLTGQ
                     IFPVRQIMQLARPRGIEVIVDGAHAYAHFPFTRDELDCDYYGTSLHKWLLAPHGTGFL
                     YVRKEKIEKIWPMMAAPPEMNANVRKFEEIGTHPAANHNAIAEALTFHQGIGAERKAA
                     RLRYLRDRWAKRLEQVPGVKLLTPADPKQSCGLASFTPGMLDVGKLTGYLWDKHRIIV
                     TAIAHPEFNCLRITPNVYTLVNEVDRFADVVAETMKKGIPA"
BASE COUNT          480 a         1120 c         1022 g          464 t
ORIGIN      
        1 gcggctaccc tgagcccatg tacgtcgtgg acatccgccc cgacacccgc gaggtcgtga
       61 tcggtccggg cgacgccctg ttcggccacg ccgtgaccct caccgacgtc aactggctcg
      121 ccgcgccgct catggccggt gaccacgtac tggtgcagtg ccgctaccgg gcgcgcgccg
      181 tccccgggac gatcgacagc atccggcccg atgcgctggc cctgcgcctg gccgagccgg
      241 tacgcgccat cacggcggga cagtccggtg tcctgtacga cgaggcggga cgggtgctgg
      301 gcggcggcct gatcgcctga actgcgcaca acaccaagcg ctcgtagcgg ttgcacaggt
      361 gcgccaggtc accgccatcg cccgcaccgg tcccgatact tggccggtcg cctgatgcac
      421 catccgtcca tcgtccggag gtcccccatg tccgttcccc gcgcactgcg ggcgtccgtc
      481 gttgtgggaa tcgcgttcgc cgccgcctgc tccgactcgt cgcatcttgg ccccgggacg
      541 ctggtcgatc ccaccgcgac ggcagccgcg ctcgcgtcct tcgacagcgc gttcgggtcg
      601 gccacgctgg cgagcttcac gtcgctgagc ctgttcatgg cgcctaccgc cgctctggcg
      661 cccgcgcgac gcgtgctcac ggccacgcag cccgtcccgt tcgccgccgg caggagcccc
      721 gaggccgccc tggcggggcg gctctccggg ctgcgcgacc tcgcgtcgct gacgctgaac
      781 ccgcagggcc cgctgattcc cgatacgctg tacggctcga tcttcacctg ggactccgcc
      841 ggcgccggct acacccggtc gcagaccacg ggcgggccgg gtaacggcgt gcggttcatc
      901 ctgtacgcgg tgaatcccct gaccggcgcg gtgtcgtatc cgctcacgcc cgtcggacag
      961 gtggacctgc tggacgagag cagcggcacg agcgcccagc tgcacatcat cgtggcgggg
     1021 aacggcggca ccccgaccta cgtcgactac acgacgacgc tcaccttcgg cctcggcacg
     1081 ctgacggcga cggtgacggg atcggtcacg aacgcgctgc agccgcccgc caacaagacg
     1141 ctgagcttca gcgccatcgc gtcgttctcg ctcaccgata tctcggtgca cgcgacgtac
     1201 acgctgaaca atccggcgtt cacggtggtg ctcgacatcg tcgacgcgaa ctcgcagccc
     1261 accgataccg tcgacgtgga cctgctgatt tcgcgaccca acgaagcggt ccgtttcacg
     1321 gcggggctcg tgaccacggg cggcgtggtc gataccgtca gcgcggcgat cacggtcaac
     1381 gcgcagctgt acgcccggat ggagggcaac gcgacggggg tcacgttcta cgacaagaac
     1441 ggcgcgccga tccaggatac cgcgacacag cacgacatcc tggtggcgct cgatcacatc
     1501 cggcacgtcg ccgagggcgt actgacgttc accgcggcgc tgttccagcc gatcatcaac
     1561 ttgctcaccg gctgagccgc cgtctcttcc cgacgccccc gccggcccct ggcccggcgg
     1621 gggcgtttgc ggtaaatccc attccactcc tctccaagga tctccggccg tgacgagccg
     1681 ccgtcgcttc ctccgctcga tgctgtccgc ctccgccgcc gttccggtcg ttcccgccct
     1741 gacgaacgac gccctgacgc acatcgcccg cacggtgggc gctgtggccg gccggccgcc
     1801 gaccgccctc gccgacgacg aggacttctg gcgcgagatc cagaacgcgt tcacgctgga
     1861 tcgcacgctc atcaacctga acaacggcgg cgtctccccc tccccacgcg tcgtccagga
     1921 ggcgatggag cgctacctcg ccatctccaa cacggcgccg gtcatcacca tgtgggcgtg
     1981 gctggagccc gagatcgaga gcgtacgtcg gcggctggcg gccgatttcg gctgcgatcc
     2041 cgaggagatg gcgatcaccc gcaacgcgtc cgaagcgctg gagatcgtcc agctcgggat
     2101 gacgttgcag cgcggcgacg aggtgctgac gaccacccag gactacccgc gcatgatcac
     2161 gacctggaaa cagcgcgagc gccgcgacgg gatcgtcctg aagcagatca ccttccccgt
     2221 gccgccgccc tcgaccgacg acctggtgcg gcgcttcgcg gaggcgatca cgccgcgcac
     2281 cagggtgatc cacgtctgcc acatcaccaa cctcacgggg cagatcttcc cggtgcgcca
     2341 gatcatgcag ctggcccggc cccggggcat cgaggtgatc gtggacggcg cccacgccta
     2401 cgcccacttc cccttcacgc gcgacgagct ggactgcgac tactacggca cgagcctgca
     2461 caagtggctg ctggcgccgc acgggaccgg cttcctgtac gtgcgcaagg agaagatcga
     2521 gaagatctgg ccgatgatgg cggcaccgcc ggagatgaac gcgaacgtcc ggaagttcga
     2581 ggagatcggg acgcacccgg cggcgaacca caacgcgatc gcggaggcgc tcacgttcca
     2641 ccagggcatc ggcgccgagc gcaaggcggc gcggctgcgc tacctgcggg accgctgggc
     2701 gaagcggctg gagcaggtgc cgggggtgaa gctcctcacg cccgccgatc cgaagcagtc
     2761 gtgcgggctg gcgtcgttca cgccggggat gctcgacgtg ggcaagctga ccggctacct
     2821 gtgggacaag caccgcatca tcgtgacggc gatcgcgcac cccgagttca actgcctgcg
     2881 catcaccccg aacgtgtata cgctggtgaa tgaggtcgat cggttcgccg atgtggtcgc
     2941 ggagacgatg aagaagggga ttcccgcgtg aagcggctat cggctatcgg ctgtcggctg
     3001 tcggccactg cgctgctgag cggggcactg ctcgtcgcgg cgtgtggcgg gggcagtagc
     3061 ggcgggagtc gcgccgcggg ggacac
//
LOCUS       DBNGZW010000172         1840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_79874, whole genome shotgun sequence.
ACCESSION   DBNGZW010000172 DBNGZW010000000
VERSION     DBNGZW010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1840
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_79874"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1333
                     /locus_tag="AC16JC_04210"
     CDS             <1..1333
                     /locus_tag="AC16JC_04210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR5163934.1"
                     /translation="WQDVTPKEIGAGTQVSMVDASPLDAATAYVAATRYKLDDFAPYA
                     YVTHDYGKTWRRITNGIRATHFVRVVRADPERQGLLYAGTEFGPYVSFDDGASWQSLR
                     QNLPVVPVHDLTIKDGDLIAATHGRSFWILDDLASLRQITPEVVAAERWLFTPGPAQR
                     GPGGGGFGAPRAVGRNPAAGARIDFYFRQAPDSATPVTLEIRDARDSLVRRFSTKPAP
                     EGDTLRVTAGLNRFVWDLRYPGAHRFRGMVLWAGDTRGPAAVPGTYTVRLAAGSWSAS
                     RTFLVREDPRVTVPVADLQRQFDFLVRIRDRVSAANDAVKQIRDLVQQVEGVTTRVKG
                     QAGAAPIRAQADSLKAHLQAVEREIYQVQNRSGEDPLNFPIRINNKLAALTGVVGSAD
                     APPTDASQAVFEALDAQLQTQLDRLHAIVTGEVPAFNRLVAEQNVPAVIVQ"
     gene            complement(1438..>1840)
                     /locus_tag="AC16JC_04215"
     CDS             complement(1438..>1840)
                     /locus_tag="AC16JC_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163935.1"
                     /translation="FNAFFGRVSGLIKERGLILSPLNVVLLSDGVPDVPPGTRLAAGE
                     NPYSRIDLSPLEYLSRSVTVRLLYASPSVGEDWKRLVRRKRVRLWTEEAQVMRGWHMQ
                     VRPDVPLEHQAALWQWMQDNVDFRVRNANLF"
BASE COUNT          280 a          628 c          661 g          271 t
ORIGIN      
        1 gtggcaggac gtgaccccga aggagatcgg cgccggcacg caggtcagca tggtggacgc
       61 ctcaccgctc gacgccgcca cggcgtacgt cgccgcgacg cgctacaagc tggacgattt
      121 tgcgccgtat gcctacgtga cgcacgacta cggcaagacc tggcgtcgca tcacgaacgg
      181 gatccgcgcc acgcacttcg tgcgggtcgt gcgggcggac cccgagcggc agggcctgct
      241 gtacgccggg accgagttcg gcccgtacgt ctctttcgac gacggcgcga gctggcagtc
      301 gctgcggcag aacctcccgg tcgtgcccgt gcacgatctg acgatcaagg acggcgatct
      361 catcgcggcg acccacggtc gctcgttctg gatcctcgac gacctcgcgt cgctgcggca
      421 gatcacgccc gaggtggtcg cggccgagcg ctggctgttc acgccggggc cggcgcaacg
      481 ggggccgggg ggcggtggct tcggggcgcc ccgcgcagtg gggagaaacc cggcggcggg
      541 tgcgcgcatc gatttctatt tccgccaggc gcccgacagc gccacgcccg tgacgctcga
      601 gatccgcgac gcgcgcgact cgctggtgcg gcgcttctcc acgaaaccgg cgcccgaagg
      661 agacaccctc cgggtcaccg cgggtctgaa ccgtttcgtg tgggatctcc ggtatcccgg
      721 ggcgcatcgc ttccggggga tggtcctgtg ggccggcgac acgcgcgggc cggcggcggt
      781 cccggggacc tacaccgtcc ggctcgcggc cggctcctgg agcgccagcc gcacgttcct
      841 ggtgcgggag gacccgcgcg tcacggtacc ggtcgcggac ctgcagcggc agttcgattt
      901 cctggtccgg atcagggacc gggtgtcggc ggccaacgac gccgtcaagc agatccggga
      961 tctggtgcag caggtcgagg gcgtgacgac gcgggtgaag gggcaggcgg gtgccgcgcc
     1021 gatccgggcg caggccgact ccctcaaggc gcacctccag gcggtggagc gcgagatcta
     1081 ccaggtgcag aatcgcagcg gagaggatcc gctgaatttc ccgatccgca tcaacaacaa
     1141 gctcgccgcg ctgacgggcg tcgtcgggtc ggccgacgcg ccgcccaccg atgccagcca
     1201 ggcggtgttc gaggcgctcg atgcccagct ccagacgcag ctggaccggc tgcacgcgat
     1261 cgtcacgggc gaggtgccgg cattcaatcg tctggtggcg gagcagaacg tgccggcggt
     1321 gattgtgcaa tgacggtggt gcaggagtaa cccgcggact catgggagtg gggagtcggg
     1381 agcggtcgga gtttgcgcca gcctgcgacc actccccgct ccccactccc tctgcttcta
     1441 gaacagattc gcgttccgca cgcggaagtc cacgttgtcc tgcatccatt gccacagggc
     1501 ggcctggtgc tcgagcggga catcgggccg gacctgcatg tgccagccgc gcatcacctg
     1561 ggcctcctcg gtccagaggc ggacgcgctt gcgacggacc agccgtttcc agtcctcgcc
     1621 caccgacggg ctcgcgtaca gcagccgcac ggtgacgctg cgtgacaggt actcgagcgg
     1681 gctcaggtcg atccgcgaat aggggttttc ccccgcggcc aaccgcgtgc cgggcggcac
     1741 gtcgggcacg ccgtcgctca gcagcaccac gttgagcggc gagaggatca gtccgcgctc
     1801 cttgatcagg cccgagaccc ggccgaagaa cgcgttgaag
//
LOCUS       DBNGZW010000173         6278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1242181, whole genome shotgun sequence.
ACCESSION   DBNGZW010000173 DBNGZW010000000
VERSION     DBNGZW010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6278
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1242181"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(150..767)
                     /locus_tag="AC16JC_04220"
     CDS             complement(150..767)
                     /locus_tag="AC16JC_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163936.1"
                     /translation="MTTRKVHLGGAAALVVALTAGGPVFANAGNSGDHYHFVSTGDFG
                     SADWSASDASGITFGWVTVSRRSADEAREGGASLSYDVARCDPSDNCVSEHGWGTLPE
                     GALTGNARSGLHLSANTAAIPGFFNSGAGGLVVVDWRRDGLVVEANSGSHTRRGEGYS
                     ERTRGTFISQSAQVSGAVLGVPIGPMASGTIGTRRSVEIDFYRER"
     gene            complement(835..1476)
                     /locus_tag="AC16JC_04225"
     CDS             complement(835..1476)
                     /locus_tag="AC16JC_04225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163937.1"
                     /translation="MNRTMIGLAGAAALAAGVIACRDAAVSPREATPAAPAFQLEGKG
                     PSHLHFVSNGDRGSADWGASDASGSVSGSLYVSRGNWADPPTTFLQYDLTRCDASHNC
                     TWEGGYGFIPNGDLTGSGAGGLHLETNTAGIPDFYNSGAGGVIVADWRRSSAWVETNK
                     GTYTIKTPAYTARNRESWVATSATVVGQMLGVVIGPGYAELGTNHAVTIDIER"
     gene            complement(1535..2788)
                     /locus_tag="AC16JC_04230"
     CDS             complement(1535..2788)
                     /locus_tag="AC16JC_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163938.1"
                     /translation="MNGRTRRLAGAVALAAGVAACGGGDAAVAPRAPAPPAPPAFAGA
                     ANQTENVHVVANGDYAYASGIATDESGGMTVFSLYVWRTGSRADPGAFISYDVGRCDA
                     VFNCVSVESGDGAIPRGDLTGSAAGGLHLETNTATNPDVVKDGPGGPIVLDWKKTGDR
                     MISYTGTQVHRTPGHTWRRQGTTTDHSAVASGEALGIAAAGGYAQLGTSHETTLEITR
                     ETGRLPLLDVAISPQSGGGPSGAPVAVGDASRVQTQSVSSWDVGSVDWSVADDGGTAG
                     SLWVRRRPQVGRTELMYNLVRCDASGTCLQQHGYGDIPDGDLTGSGAGGLHLRTNTAG
                     LPDFYNSGAGGVIEVDWKRTALWLDVLTGGATWRYPTYTERRRGRETEVSATASGHVF
                     GVPIPPGADASVGTDQTVRILREEP"
     gene            complement(2933..4405)
                     /locus_tag="AC16JC_04235"
     CDS             complement(2933..4405)
                     /locus_tag="AC16JC_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163939.1"
                     /translation="MKPKAEIPPAVARETSDPTLTATPSATTSITNAYPGPSAVPFLP
                     VTLLPTPTREYRERRKQRHATLATGTPRRMPIPPPAISGSRVVLWQWNPSLGQSTVRG
                     AFLPGQLLPGPRDARITIAGSPPVIPNVLGDLIAAPGSEAFDAIHTFAVVRQTLTMFQ
                     RALFPTSAPWQWNSRGNTDPIEVYPRAGETMNAYYSRKERALKFYYFTPPGIVAEGEA
                     SRVYACRSQDLVAHATGHAVLDGLKPSWILTQHPPQTGALHEAFADLTAIFLMLTTPE
                     QVAAIVTQTKANLHNKLFLDDLVEDMGLVLGRPVGLWNVEQELTLSQVGTEVHDLALV
                     FTGAIYDVLADIVTFELKANSDPAATVYAAAEYMCGLLIRAIKAAPDESAGFAEVVNQ
                     MLRVVELDEKPVQYRNFIRNRFVQREVVTSSTSLGADHAAGRELEAKPRDADDAVQIR
                     LACCGTLQQPQYAGLDTVAPEVEEFVKGLSGSTQSQGSRR"
     gene            complement(4526..5239)
                     /locus_tag="AC16JC_04240"
     CDS             complement(4526..5239)
                     /locus_tag="AC16JC_04240"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR5163940.1"
                     /translation="MTTKLTHKTPPTPPVGVLLVGDVRLVREALALWFRDQGPLRLIA
                     VAGHRDAALALAREHRPAPDLALIDMRMPDGAELAQALASACPALKLVGFGLSETEDN
                     VLQYAGAGFAGYVPREASLADLVDVLQGIARGEVRYSRRITTSLLHRLATVVLPNGGA
                     VQLTPREAEIARLIDDGLTNRAIARRLAIEVATVKNHVHHLLEKLKAGRRGEAVARLR
                     HPGHLPPPSAGGTVPDRRI"
     gene            5476..>6278
                     /locus_tag="AC16JC_04245"
     CDS             5476..>6278
                     /locus_tag="AC16JC_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5163941.1"
                     /translation="MIPTDAGLLFVTRSVRLFAYGSLSVILVFYLTSLGLSTTQTGLL
                     LTLTLVGDTAVSLWLTTRADRIGRRRMLVVGALLMAAAGVAFAGTRNFLFLIVAGTLG
                     VISPSGNEVGPFLPIEQAALSQVLPDHRRTHGFAWYSLAGSFATALGSLCGGVVSGAL
                     QHATVSPLGSYRAVVFAYAGLGVVLTLLFGRLSPAVEVRSATRAASGIGRSRGMILRL
                     SSLFALDAFAGGFVVQSFAAYWFYLRFGVDPATLGGIFLGANLFAGVSAL"
BASE COUNT          857 a         2298 c         2105 g         1018 t
ORIGIN      
        1 gacccgtccc ggcaggccgc cacgccgccc gccagcgccg ccgcccccgc cagccagatc
       61 atcgtactgc tcataggaac gtccttttcc gatgcgtcca acctccacca tcctccaccg
      121 tcctccaccg tcctcaacca acctccctct caccgctccc ggtagaagtc gatctctacc
      181 gagcgcctcg tcccgatcgt ccccgaggcc atgggaccga tcggcacccc cagcaccgct
      241 cccgagacct gggccgactg ggagatgaaa gtcccccgcg tgcgctccga gtagccctcg
      301 ccccggcgcg tgtgcgagcc ggagttcgct tccaccacca gcccgtccct gcgccagtcc
      361 acgaccacga gaccaccggc gccggagttg aagaacccgg ggatggcggc ggtgttggcg
      421 ctcagatgca gcccgctccg ggcattcccg gtcagggcgc cctcggggag ggtcccccac
      481 ccgtgctcgg acacgcagtt gtcggatgga tcgcaccgcg cgacgtcata ggacagcgac
      541 gctcccccct cccgcgcctc gtccgcgctg cggcgcgaga cggtgaccca gccgaacgtg
      601 atccccgacg cgtcgctcgc gctccaatcc gcggacccga agtcgcccgt ggagacgaag
      661 tggtagtggt cgccggaatt gcccgcattc gcgaacaccg gcccgccagc cgtgagggcg
      721 accaccagcg ccgccgcgcc gccaaggtgc accttgcgag tcgtcatcgt gctccttcct
      781 ttcctgacct ccaccaacct ccaccgtcct ccaccgtctt cccccgccct tcttctatct
      841 ctcgatgtcg atcgtcaccg cgtggttcgt ccccagctcg gcatagccgg ggccgatcac
      901 gacccccagc atctgcccga ccaccgtcgc cgacgtggcg acccacgact cccggttgcg
      961 cgccgtgtac gcgggggtct tgatcgtgta ggtgcccttg ttcgtctcca cccaggcgct
     1021 gctgcggcgc caatccgcca cgatcacgcc gcccgcgccg ctattgtaga aatcgggaat
     1081 cccggcggtg ttggtctcga ggtgcagccc gccggcacca ctcccggtga ggtccccgtt
     1141 ggggatgaag ccgtagccgc cctcccaggt gcagttgtgg gacgcatcgc accgcgtgag
     1201 gtcgtattgg agaaaggtcg tcggcgggtc ggcccagttc ccgcgcgaca cgtagagcga
     1261 gccggagacg ctgccgctcg cgtcgctcgc gccccaatcg gcggacccgc ggtcgccgtt
     1321 ggagacgaag tgaaggtgtg acggtccttt cccctcgagc tggaacgccg gcgcggccgg
     1381 cgtcgcctcg cggggagaca ccgcggcgtc cctgcaggct atcactccgg ccgccagcgc
     1441 cgcggcgccc gccagcccaa tcatcgtcct gttcatgcgc ccgtcctttc tccgacctcc
     1501 accatcctcc accgtcctcc accgactttt tttctcaggg ctcctctctc agaatcctca
     1561 cggtctggtc cgtcccgacc gaggcgtcgg cgccgggcgg gatcggcaca ccgaagacat
     1621 ggcccgacgc cgtggcggag acctctgtct ccctgccccg acgccgttcc gtatacgtcg
     1681 ggtaccgcca cgtggcgccg cccgtcagga cgtccagcca gagcgcggtc cttttccagt
     1741 cgacctcgat gaccccgccc gcaccggagt tgtagaagtc ggggagcccg gccgtgttgg
     1801 tgcgcagatg cagcccgccc gctccgctgc ccgtgagatc cccgtcgggg atgtcgccgt
     1861 agccgtgctg ctgcaggcag gtaccggacg catcgcaccg cacgaggttg tacatcagct
     1921 cggtccgccc gacctggggc cggcgtctca cccacaggga gccggccgtg ccgccgtcgt
     1981 ccgcgacgct ccagtccacg gatcctacgt cccagctgga gacggactgg gtctgcaccc
     2041 tcgaggcgtc tcccacggcg actggcgcgc cggacggccc gccgccggat tgcggcgaaa
     2101 tcgccacgtc cagaagcggc aggcgccccg tttcccgggt gatctcgagc gtggtctcgt
     2161 ggctcgtgcc gagctgggcg tagccgccgg cggccgcgat ccccagcgcc tcccccgacg
     2221 ccacggccga atggtccgtc gtcgtcccct gccggcgcca cgtgtgcccg ggcgtgcggt
     2281 gcacctgggt gccggtatag gagatcatcc ggtccccggt cttcttccag tcgagcacga
     2341 tcggaccgcc cgggccgtcc ttgaccacgt ccggattggt cgcggtgttg gtctcgaggt
     2401 gcagcccgcc cgcggcgctg ccggtgaggt cgccgcgggg gatcgcgccg tcgccggact
     2461 ccaccgagac gcagttgaag acagcatcgc agcgcccgac gtcgtaggag atgaatgctc
     2521 ccggatctgc gcgcgagccg gtgcgccaga cgtacagcga gaagacggtc ataccgcccg
     2581 actcgtccgt cgcgatcccg ctggcgtagg catagtcgcc gttggcgacg acgtggacgt
     2641 tctccgtctg gttcgccgcc ccggcgaatg cgggcggcgc gggcggcgcg ggcgcgcgcg
     2701 gggcgaccgc ggcgtctccc cccccgcacg ccgcgacgcc ggccgccagc gctaccgcgc
     2761 cggccagccg cctggtacga ccgttcatat gccgctcctt gtcggacgaa tgctgaagag
     2821 gagttcaagc tgcggtgggc ggcgtgaaac ggcgttgaag cggagaataa aggaggttga
     2881 ggaggaggac tgtggaggac ggtgacaccg tcctccaccg ccggtactgc tcctagcgcc
     2941 ggctcccctg cgactgcgtc gagccggaga gccccttgac gaactcctcc acctcgggcg
     3001 cgacggtgtc gagcccggcg tactgcggct gctgcagcgt gccgcaacag gccagacgaa
     3061 tctggaccgc gtcgtccgcg tcccgcggct tcgcctccag ctcccgcccg gcggcgtgat
     3121 cggcgccgag cgacgtcgac gatgtcacga cctcccgctg cacgaagcgg ttccggatga
     3181 aattgcggta ctgcaccggc ttctcgtcca gctccaccac ccgcagcatc tggttcacga
     3241 cctcggcgaa ccccgcggac tcgtccggcg ccgccttgat ggcccggatc agcaggccgc
     3301 acatgtactc ggccgccgcg tacaccgtcg ccgccggatc gctgttcgcc ttcagctcga
     3361 acgtcacgat gtccgccagc acgtcgtaga tcgcccccgt gaacaccagg gcgagatcgt
     3421 gcacctcggt ccccacctgg gagagcgtca gctcctgctc cacgttccac agccccaccg
     3481 gccgccccag caccagcccc atgtcctcga ccagatcatc gagaaacagc ttgttgtgga
     3541 gattggcctt cgtctgcgtc acgatcgccg ccacctgctc cggggtcgtc agcatcagga
     3601 agatcgccgt caggtccgcg aaggcttcgt gcagcgcgcc ggtctgcggc ggatgctgcg
     3661 tcaggatcca gctcggcttg aggccgtcca gcaccgcgtg acccgtcgcg tgcgccacca
     3721 ggtcctgcga gcggcaggcg tagacgcgcg acgcctcccc ctccgcgacg atgccgggcg
     3781 gcgtgaaata gtagaacttg agcgcccgct ccttgcggct gtagtaggcg ttcatcgtct
     3841 ccccggcccg cggatagacc tcgatcgggt cggtgttccc ccggctgttc cattgccacg
     3901 gcgccgaggt gggaaacagg gcgcgctgga acatggtcag cgtctgccgc accaccgcga
     3961 aggtgtggat cgcgtcgaac gcctccgacc ccggcgcggc aatcaggtcc cccaggacgt
     4021 tggggatcac cgggggcgac ccggcgatcg tgatgcgcgc gtcgcgcggg ccgggcagca
     4081 gctgccccgg gaggaacgcg ccgcgcacgg tgctctggcc caggctgggg ttccactgcc
     4141 acagcaccac ccgcgacccg cttatcgccg gcggcgggat cggcatgcgg cgcggcgtgc
     4201 cggtcgccag cgtggcgtgg cgctgcttgc ggcgctcccg gtactcgcgc gtcggggtgg
     4261 gcagcagcgt caccgggaga aacggcacgg cgctcgggcc gggatatgca ttcgtgatgg
     4321 acgtcgtggc ggacggcgtg gccgtcaggg tggggtcgga cgtctcgcga gcgaccgccg
     4381 gagggatttc ggcttttggc ttcacggtcg tctcctgtgc ttgtggagga tgaaggatcc
     4441 gcacgggcgt gcgggctgac acgtccgtgc agcctcgtca atctccacag cgaccggaat
     4501 ggactcaggg aacgatggcc tggatctaga tccttcgatc cggcacggtc ccaccggcgg
     4561 atggcggcgg gaggtggccg gggtgacgca gccgggcgac ggcttccccg cggcggccgg
     4621 ccttgagctt ctcgaggaga tggtggacat ggttcttcac cgtcgcgacc tcgatcgcca
     4681 ggcggcgcgc gatcgcgcgg ttggtgagcc cgtcgtcgat cagccgcgcg atctccgcct
     4741 cccgcggcgt gagctgcacc gcgccgccgt tgggcagcac cacggtcgcc agacggtgca
     4801 gcagcgacgt ggtgatgcgc cgcgagtagc gcacttctcc ccgggcgatc ccctggagca
     4861 cgtcgaccag atctgcgagc gacgcctctc gcggcacgta gccggcgaac cccgcccccg
     4921 cgtactggag cacgttgtcc tccgtctcgg acaggccgaa gcccacgagc ttgagcgcgg
     4981 ggcaggccga cgcgagcgcc tgggccagct ccgccccgtc gggcatgcgc atgtcgatca
     5041 gtgccaggtc gggggccggg cggtgctcgc gcgccagcgc gagggcggcg tcgcggtgcc
     5101 ccgccacggc gatgagccgc agcggcccct ggtcccggaa ccacagggcg agcgcttccc
     5161 ggaccagccg cacgtcgccc acgagcagca cccctaccgg cggcgttggc ggcgtcttgt
     5221 gcgtgagctt cgtcgtcatc cgtgggcgag tgggcgagtg ggcgagtggg cgaatcggtc
     5281 gccggaaacc caccccggcc gggagtcggg gggggctgcg cacggccagc actggttata
     5341 gcggcggagc gggaaaaagg tccgggacgg tggaggacgg tggaggacgg tggaggacgg
     5401 tggaggacgg tggaggctac cgtcgtagat tgaccgacgt gcacggccat cccgtattgc
     5461 gcgtgctgga gccgagtgat tcccactgac gccgggctgc tgttcgtcac gcgatcggtg
     5521 cggctgttcg cgtacgggtc gctctccgtc attctcgtct tctatctcac cagcctcggc
     5581 ctgagcacga cccaaacggg cctcctgctc accctcacgc tcgtcgggga taccgccgtc
     5641 tccctctggc tcacgacgcg cgccgaccgg atcggacggc gccgcatgct ggtcgtcggc
     5701 gccctgctga tggccgctgc cggtgtggcg ttcgccggca cgaggaactt cctcttcctc
     5761 atcgtcgccg ggacgctcgg ggtcatcagc cccagcggca acgaggtcgg accgttcctc
     5821 ccgatcgagc aggcggcgct gtcgcaggtg ctgcccgatc accggcggac ccatggcttt
     5881 gcctggtact ccctggccgg ctcgttcgct accgcgctcg gctcgctgtg cggtggtgtc
     5941 gtttccggcg cgctgcagca tgcgaccgtc tcgccgctgg ggagttaccg cgcagtggtc
     6001 ttcgcctacg cagggctggg ggtggtgctc accctcctgt tcggccgcct ctcgccggcg
     6061 gtggaagtgc ggtccgcgac cagagcggcc tccggcatcg gccggtcgcg cgggatgatt
     6121 ctcagactct ccagcctgtt cgccctcgat gcgttcgccg gcggcttcgt cgtgcagagc
     6181 ttcgcggcgt actggttcta cctccgtttc ggtgtggatc ctgcgacgct gggtggcatt
     6241 ttcctgggcg cgaacctctt cgccggcgta tcggccct
//
LOCUS       DBNGZW010000174         3075 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1585479, whole genome shotgun sequence.
ACCESSION   DBNGZW010000174 DBNGZW010000000
VERSION     DBNGZW010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1585479"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="AC16JC_04250"
     CDS             <1..512
                     /locus_tag="AC16JC_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007085212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR04190 family B12-binding domain/radical SAM
                     domain protein"
                     /protein_id="HIR5163942.1"
                     /translation="PGSPAFEQPERFGYRKLCRTFEDHRRALMGPSWKHILSFETDSM
                     TRDEIVDATYEASLRLAKAKLDFGIIDEVSYRKLVSGVAASRQLVSEIDAIQALPDAA
                     ERQARLAAVRQRVTDLARPEVRAKDWLKWPIRRRFARLHRLVRMLVGLFVVEIRQFLF
                     KRLRLVFSH"
     gene            complement(603..2195)
                     /locus_tag="AC16JC_04255"
     CDS             complement(603..2195)
                     /locus_tag="AC16JC_04255"
                     /inference="COORDINATES: protein motif:HMM:NF024827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5163943.1"
                     /translation="MKNARWLATALVVTALGACTNEKIVYQTREPFNPPPDGAFLGYF
                     DIGTKQTTCGNCHVGVQANWVSTKHSEAWVDLQASGHATGSCNACHSVSELGNDAGAP
                     AGYSVKQDSVYHDVQCESCHGAGFTHVQSPTAVNEPLASMKADTTGTNGCGACHTGNH
                     EPYVDQWIQSAHGSGEALSHAGANAACAPCHEGQAALVTKFGVTGNFAEKGSTELQRI
                     TCVVCHDPHGGPKEGQLRASIATPTTDNLCVTCHSRKGTPPWGVAAVGGTARGSHGAQ
                     GLLVIGQNVGWFPPNFAYDTTLIVSSHGTEANPRLCATCHVAQTTITDKLTGAFQFQS
                     VGHLFEAIPCLDATGKPVPGTCAITQRDFSACSNAACHIGGPATARGAYISVITNLNN
                     LLNQLWNDANANDTIDATDTGLLPAMVARGTAGDTAALNFGTNVTTTAKGALFNAALA
                     ATEDKAYFLSGTVYGRTFATHMASGNGVHNPFLLQALLAASIDAVRTEYALPAPPNYD
                     THIVGTTPASVRPRVASQEANR"
     gene            complement(2314..2943)
                     /locus_tag="AC16JC_04260"
     CDS             complement(2314..2943)
                     /locus_tag="AC16JC_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HIR5163944.1"
                     /translation="MSAASLAPTRLNLVRAVRRLERLSQGAAVLRRKREALVTELFRL
                     ARPAADARQQIAAAAQRAYPLLAGALALQGQAGVRAIGWPGREVSVDMEAGSVWGVVV
                     SRIESLPAIARTLGARGTAPAGTGPVAVQAATEFEKLTQLLLDAAPREMLLRRLGQAL
                     AQTSRQVHSLEHRLSPRLRGDIARVRQVLDEREREERLRLQTLTRTRRR"
BASE COUNT          419 a         1065 c         1083 g          508 t
ORIGIN      
        1 accccggcag cccggcgttc gagcagccgg agcggttcgg gtaccggaag ctgtgccgga
       61 ccttcgagga ccatcgccgc gcgctcatgg gcccgagctg gaaacacatc ctgagcttcg
      121 agaccgacag catgacccgg gacgagatcg tggacgcgac ctacgaggcg tcgttgcgcc
      181 tcgcgaaggc caagctcgat ttcgggatca tcgatgaggt gtcctatcgg aagctggtgt
      241 ccggcgtcgc cgcctcgcgt cagctcgtgt cggaaatcga cgcgatccag gcgctccccg
      301 acgcggccga gcggcaggcg cgtctcgccg cggtgcggca gcgcgtgacg gatctcgccc
      361 gtccggaggt ccgcgccaag gactggctca agtggccgat ccggcgccgc ttcgcgcgcc
      421 tgcaccggct cgtgcggatg ctggtcgggc tgttcgtcgt ggagatccgg cagttcctgt
      481 tcaaacggct gcgcctggtt ttctcgcact agcccgagca ccccgcggac gcaagaacga
      541 cgggccccgg tctctcgacc ggggcccgcg cttcgttcag accgtagcgg cgtgacgacg
      601 cctcagcggt tggcctcctg gctcgcgact ctgggccgga cactcgccgg cgtcgttccg
      661 acgatgtgcg tgtcgtagtt cggcggcgcc ggcagggcgt attccgtccg gacggcgtcg
      721 atcgacgccg ccaggagcgc ctggagcagg aacgggttgt gcacgccgtt gccggacgcc
      781 atgtgggtgg cgaaggtcct cccgtacacc gtgccgctca ggaagtaggc cttgtcctcc
      841 gtcgcggcga gcgcggcatt gaacagcgcg cccttggcgg tggtcgtgac gttggtgccg
      901 aagttgagcg cggcggtgtc gccggccgtg ccccgcgcca ccatcgccgg cagtaggccg
      961 gtatcggtcg cgtcgatggt gtcgttggcg ttggcatcgt tccacagctg gttcagcagg
     1021 ttgttcaggt tcgtgatgac gctgatgtag gccccccggg cggtcgccgg ccccccgatg
     1081 tggcaggctg cgttggagca ggcgctgaag tcgcgctgcg tgatggcaca cgtgccgggc
     1141 accggcttgc cggtggcgtc gaggcaggga atcgcctcga acaggtgacc gacgctctgg
     1201 aactggaacg cgcccgtgag cttgtcggtg atggtggtct gcgccacgtg gcaggtcgcg
     1261 cacagccgtg ggttcgcttc cgtcccgtgg ctgctcacga tcagggtcgt gtcgtaggcg
     1321 aagttcgggg ggaaccagcc gacgttctgg ccgatcacca gcagcccctg cgcgccgtgc
     1381 gagccacggg ccgtaccgcc gaccgccgcc acgccccacg gcggcgtgcc tttgcgggag
     1441 tgacaggtca cgcagaggtt gtcggtcgtc ggcgtggcaa tggacgcccg cagctggccc
     1501 tctttgggcc cgccgtgggg gtcgtggcac acgacgcaag tgatgcgctg caattccgtc
     1561 gagcccttct cggcgaagtt gccagtgacg ccgaacttcg tcaccagtgc ggcctggcct
     1621 tcgtgacagg gcgcgcacgc cgcgttcgct ccggcgtgac tcaacgcttc gccggaaccg
     1681 tgggccgact ggatccactg atcgacgtac ggctcgtggt tgccggtgtg gcacgcgccg
     1741 cagccgttcg tcccggtggt gtcggccttc atcgacgcga gcggctcgtt caccgcggtc
     1801 gggctctgga cgtgcgtgaa tcccgcaccg tggcagctct cgcactgcac gtcgtgatag
     1861 accgagtcct gcttgacgct gtagcccgcg ggggcgccgg cgtcgttgcc cagctcggac
     1921 acgctgtggc acgcgttgca ggagcccgtc gcgtgccccg acgcctggag atcgacccac
     1981 gcctcggagt gcttcgtcga cacccagttc gcctgcacgc ccacgtgaca gttcccacag
     2041 gtggtttgtt tcgtgccgat gtcgaagtag ccgaggaaag cgccgtcggg gggcggattg
     2101 aacggctcgc gtgtctgata gacgatcttt tcgttcgtac acgcgccgag cgcggtcacg
     2161 acgagcgcgg tggcgagcca acgggcgttc ttcataacta gggtcctttc cttcgagtgc
     2221 cacggctgca ggtccggcgt tcactaccta cgtacacata ctgcctgacg cgcatgaggg
     2281 cataatgaag cactactgac gatctcatgg acctcaccgc cggcgcgtgc gtgtgagcgt
     2341 ctgcagtcgc aagcgttctt cacgctcccg ctcgtcgagc acctgtcgca cccgcgcgat
     2401 gtctccccgc agccgcggcg acaggcggtg ttcgaggctg tgcacctgac gcgacgtctg
     2461 ggcaagcgcc tgccccagtc gccgcagcag catctcgcgc ggcgcggcgt cgagcagcag
     2521 ctgggtgagc ttctcgaact cggtggccgc ctggaccgcg accggccccg tgcccgccgg
     2581 ggcggtgcca cgggcaccca atgtccgggc gatggccggc agcgactcga tgcgcgacac
     2641 caccacgccc cacaccgatc ccgcctccat gtcgaccgac acctcccgac ccggccatcc
     2701 gatggctcgc actccggcct ggccctgcaa cgcgagcgcg cctgccagca acgggtaggc
     2761 ccgctgcgcc gcggcggcga tctgctgccg ggcgtcggcc gcgggccggg cgaggcggaa
     2821 cagctccgtg acgagggcct cgcgcttgcg gcgcaggacc gccgcgccct gcgacaggcg
     2881 ctccagccgc cggaccgcgc gcaccagatt gagccgcgtg ggagcgagac tcgcggcgct
     2941 catgcgctgc gctcggcggg atgccgggcc tcccaggtcg cgtcgctcag ccgcatgagg
     3001 tcctcgcgcg gcagcgtgtc gagcagccgc cagccggccg cgaacgtctc ggcgagcgcg
     3061 cgccgcgcgg cgccc
//
LOCUS       DBNGZW010000175         6219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1516214, whole genome shotgun sequence.
ACCESSION   DBNGZW010000175 DBNGZW010000000
VERSION     DBNGZW010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6219
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1516214"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="AC16JC_04265"
     CDS             complement(<1..462)
                     /locus_tag="AC16JC_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024211.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR5163945.1"
                     /translation="MLGFVERKWIYEQAVTGPWQRLRKATFAALHVILFVTQWITVNG
                     SPALRIDLPGRRLYAFGAIFTAADTVFVLLLLLFLAFALFFFTSLFGRLWCGFACPQT
                     VFLDTWIRPLEEWLEGDWVQRRKRDQAGWSLDRLGRKSLKWALFLAIALAVS"
     gene            complement(465..1031)
                     /locus_tag="AC16JC_04270"
     CDS             complement(465..1031)
                     /locus_tag="AC16JC_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5163946.1"
                     /translation="MSKERTRDQVLGHAAEADGIEEFDNRMPNWWLGLFYATIVWAVA
                     YGVHYHVIAHRSQVKQLAAEMAAADRRWPRQAAAAADTTLALTHEAVEAGEAIFKANC
                     VSCHGEDMRGKIGPNLLDTIWIHGGRPQDILRTITVGVPEKGMITWGPILGSEKVAQV
                     AAYVVEKNREATGGKDEEHEHDREEEHH"
     gene            complement(1028..1207)
                     /locus_tag="AC16JC_04275"
     CDS             complement(1028..1207)
                     /locus_tag="AC16JC_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CcoQ/FixQ family Cbb3-type cytochrome c oxidase
                     assembly chaperone"
                     /protein_id="HIR5163947.1"
                     /translation="MNPLLDAAAGSVTLGWVLGVTTLMFLGGFLFWVWWAWAGRNKAR
                     WDADSRLPFDDGGES"
     gene            complement(1204..1914)
                     /gene="ccoO"
                     /locus_tag="AC16JC_04280"
     CDS             complement(1204..1914)
                     /gene="ccoO"
                     /locus_tag="AC16JC_04280"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF011055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HIR5163948.1"
                     /translation="MNESGGRGYTALHRVLEGKGALFAILATVVILIGGMVEIVPMFS
                     ARTGPEMLAGVTPYSPLEVAGRDIYIREGCYTCHSQMIRPLRAEVLRYGEWTRAGEYA
                     YDHPFLLGSRRAGPDLQRVGGKYPDAWHFEHMRDPRSTSPGSIMPPYPWLYDRRYDPA
                     DIQASLRALRRVGVPYGEADIATAPAAMQQQAEAIVARLGQSGITTTTDREIVALIAY
                     LQRLGTDGRAAIEAGRSR"
     gene            complement(1911..3374)
                     /gene="ccoN"
                     /locus_tag="AC16JC_04285"
     CDS             complement(1911..3374)
                     /gene="ccoN"
                     /locus_tag="AC16JC_04285"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045277 - respiratory chain complex IV
                     [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit I"
                     /protein_id="HIR5163949.1"
                     /translation="MEYTGVRNGDEPRYDNQIVKMFVTATVVWAVVGMLVGVWIALEL
                     AWWPANLGLSFTTFGRLRPLHTNAVIFAFAGNVCFFGIYHSMQRLLKTRMYSDLLSRL
                     HFWDWQLVIVCAAITLPLGLTQSKEYAELIWPIDVLITVVWVIFAVNFFGTIAIRKTQ
                     HLYVAIWFYMSFVITIAVLHIVNSLAVPATWLRSYPVYAGLTDALVQWWYGHNAVGFF
                     LTTPFLGLMYYYLPRISGRPIFSYRLSIVHFWALIFLYIWAGPHHLLFSALPEWAQTL
                     GMVFSIMLLAPSWGGMLNGLLTLRGAWDRVRTDPILKFLVVAVSFYGMSTFEGPMMSI
                     RTVNSLSHNTNWTIGHVHSGALGWVGMISFGVLYWVIPRVWNRRLAHPDWPAIHFWLA
                     TIGIVLYTVSMWAAGLTEGLEWRAFDDAGQLRYPNFVDIVHQLAPFYWLRLAGGLLYF
                     TGAIMLAVNFFKTVTGRSPAPATAAPAAAGTEGGAAS"
     gene            3381..4043
                     /locus_tag="AC16JC_04290"
     CDS             3381..4043
                     /locus_tag="AC16JC_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5163950.1"
                     /translation="MVLLGAAFLTGLLGSVHCVAMCGAFAASCTRVPRGLPAWHAGRI
                     GTYALLGALAGAAGRLLPGPPWLPPAVAASLLLWFALALAGVVPEPRLIPRGLTNAAS
                     RAAQTPSPAAQAAFGVANGFLPCGLVYSALSIPVALADPVRGALAMIAFGTGTLPALS
                     LAAVGLRRLVMTSLWRRRVFALLVLVAGAWAIWTRATGAATAGHHHAPAAPPARQNPG
                     SD"
     gene            complement(4138..4992)
                     /locus_tag="AC16JC_04295"
     CDS             complement(4138..4992)
                     /locus_tag="AC16JC_04295"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5163951.1"
                     /translation="MPEGSAANAPRQKRIRWGRWSRLSLVGIVGVTMALPFLYMLTTS
                     LMDEFQVMRYPPAILPAHPKFDNYGAALTALPFWRFFVNSAFVSIVVVTGQVLTSAAA
                     AYAFARLRFPGRDRIFLLFLAVLMVPTVVLLIPRFLIVSTLGWVDSYAGLITTELVTV
                     WGIFLLRQFFQTLPRELEDAARLDGAGEWQIFWKVVLPLSRPALATLAVFGLVDQWKS
                     FLWPLVVTRSVRMQVVEVGIASFHGIYYTNWPYQMAAAVTAVVPLVVLFFIAQRYFVK
                     GIQLTGLK"
     gene            complement(4985..>6219)
                     /locus_tag="AC16JC_04300"
     CDS             complement(4985..>6219)
                     /locus_tag="AC16JC_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5163952.1"
                     /translation="AVQGRKDATTRWLSQTGLLLEEAAARFPVRVWPPLDRLARLAPE
                     SDLAVADSGPTDVRLVTVAGVPRAEIRVRLGFDRWVVLRDFAGDNDGGWLVLTLALAA
                     MGQLGALLLGWIERSRARPRALRETLAAWGFLAPATLHLVAFSFGPMLFALYLALHRW
                     SPIEPARPFVGLANFVTVVRDPLVWTSLRNTLLYSLHVPVAMAIALGMALLLRRHSRA
                     TRLVRTVFFLPYVSSAVAIALVWQWMYHPDFGLLNYFVGLVGIRPVDWLGNPRTALLA
                     VMLVSIWVQVGYQMVVFQAGLQGIPREYLDAARVDGANAWRRFWRVTFPLLRPVTLFV
                     LVTGVITSFQVFTLVYVLTDGGPLHATDVLVYRIYQLAWEFLQFGPASALALLLFALL
                     FVATWVQFRLLGKQVQYA"
BASE COUNT         1128 a         2154 c         2069 g          868 t
ORIGIN      
        1 cgacacggcg agcgcaatcg cgagaaacag cgcccacttg agactcttgc gccccagccg
       61 gtcgaggctc catcccgcct gatctcgctt ccgccgctgc acccagtcgc cctcgagcca
      121 ctcctccagc ggccggatcc aggtgtcgag gaacacggtc tgcgggcagg cgaatccgca
      181 ccacagccgc ccgaacaacg acgtgaagaa gaagagggcg aatgccagga acaggagcag
      241 caggagcacg aacaccgtgt cggcggcggt gaaaatggcg ccgaacgcat acaaccggcg
      301 gcccggcaga tcgatgcgca gcgcggggct gccgttcacg gtgatccact gggtcacgaa
      361 caggatgaca tgcagcgcgg cgaaggtcgc cttgcgcaac cgttgccacg gccccgtgac
      421 ggcctgctcg tagatccact tccgctcgac gaaccccagc atgctcagtg atgctcctcc
      481 tcacggtcgt gctcgtgctc ttcgtccttc cccccggtcg cctcgcggtt cttctccacg
      541 acgtaggcgg ccacctgggc gactttctcg gatccgagaa tcgggcccca ggtgatcatc
      601 cctttttccg ggaccccgac ggtgatggtg cgaaggatat cctgcggccg gccgccgtgg
      661 atccagatgg tatcgagcag attgggaccg atcttcccgc gcatgtcctc gccgtggcac
      721 gagacgcagt tggccttgaa gatcgcttcg cccgcctcca cggcctcgtg ggtcagcgcg
      781 agggtcgtgt cggcggccgc cgcggcctgt cgcggccagc gccggtcggc ggcggccatc
      841 tccgccgcga gctgtttgac ctgggagcgg tgcgcgatga cgtgataatg caccccgtac
      901 gccaccgccc agacgatggt cgcgtagaac agccccagcc accagttggg catccggttg
      961 tcgaactcct cgatcccgtc cgcctcggcg gcgtggccca ggacctgatc gcgtgtccgt
     1021 tccttgctca cgactctcct ccgtcgtcga atgggaggcg gctatccgca tcccagcgcg
     1081 ccttgttgcg gcccgcccag gcccaccaga cccagaacag aaagccgccg agaaacatca
     1141 gagtcgtcac gcccaggacc cagccgagcg tgacgctgcc cgcggcggca tcgaggagcg
     1201 gattcatcgg gaccgcccgg cttcgatcgc cgcccggccg tccgtgccca ggcgttgcag
     1261 ataggcgatc agcgccacga tctcccggtc ggtcgtggtg gtgatgccgg actgtccgag
     1321 tcgggcgacg atcgcctcgg cctgctgctg catcgcggcc ggcgcggtcg cgatgtccgc
     1381 ctcgccgtag ggcacgccca cccgccgcag cgcccgcagc gacgcctgga tgtcggccgg
     1441 gtcgtagcgc cgatcgtaga gccaggggta gggcggcatg atgctgcctg gcgacgtgct
     1501 gcgcggatcg cgcatgtgct cgaaatgcca ggcgtcggga tacttgccgc cgactcgctg
     1561 cagatcgggg ccggcgcggc gcgagccgag caggaaggga tggtcgtagg catactcgcc
     1621 cgcgcgggtc cactcgccgt agcgcagcac ttccgcgcgc agcgggcgga tcatctggga
     1681 gtggcaggta tagcaccctt cccggatgta gatatcgcga ccggcgacct cgagcggcga
     1741 atagggtgtc acacccgcca gcatctccgg gccggtgcgg gccgagaaca tcgggacgat
     1801 ttcgaccatg ccgccgatca ggatcaccac ggtcgcgagg atcgcgaaca acgcgccctt
     1861 cccctccagg acgcggtgca gcgcggtgta gccgcgcccg ccggactcgt tcatgacgcc
     1921 gctccccctt ccgttccggc agccgccgga gccgccgtcg ccggtgcggg actccgcccg
     1981 gtgacggtct tgaagaaatt caccgccagc atgatggcgc cggtgaagta gaggagtccg
     2041 cccgcgaggc gcagccagta gaacggcgcg agctggtgga cgatgtccac gaaattcgga
     2101 tagcggagct ggcccgcgtc gtcgaaggcg cgccattcca gtccctcggt gagtcccgcc
     2161 gcccacatcg acaccgtgta caggacgatg ccgatcgtcg cgagccagaa gtggatcgcg
     2221 ggccagtccg gatgcgccag acggcggttc cacacccggg gaatcaccca gtacagcaca
     2281 ccgaaggaga tcatccccac ccaccccagt gctcccgagt gcacgtggcc gatcgtccag
     2341 ttggtgttgt gcgagaggct gttcaccgtg cggatggaca tcatcggccc ctcgaacgtg
     2401 ctcatcccgt agaagctcac ggcgacgacg aggaacttga gaatgggatc ggtacggacc
     2461 cggtcccagg cgccgcgcag ggtcagcagg ccgttcagca tcccgcccca cgagggcgca
     2521 agcagcatga tgctgaagac cattcccagc gtctgcgccc actcgggcag cgccgagaag
     2581 aggaggtggt gcggtccggc ccagatgtag aggaagatca gcgcccagaa atgcacgatc
     2641 gacaggcgat aactgaagat cggccgcccg gagatgcgcg ggagatagta gtacatgagc
     2701 cccaggaacg gcgtggtgag aaagaatccc accgcgttgt gcccgtacca ccattgcacc
     2761 agcgcgtcgg tgagtcccgc gtacaccgga taggagcgca gccacgtcgc cgggacggcg
     2821 aggctgttca cgatgtgcag cacggcgatc gtgatcacga acgacatgta gaaccagatg
     2881 gcgacgtaca gatgctgcgt cttgcggatg gcgatcgtgc cgaagaagtt gacggcgaag
     2941 atcacccaga cgacggtgat caggacgtcg atcggccaga tcagctcggc atactccttg
     3001 gattgggtga ggccgagcgg cagggtgatc gcggcacaca cgatcacgag ctgccagtcc
     3061 cagaagtgga gccgcgagag cagatcgctg tacatccgtg tcttgaggag ccgctgcatc
     3121 gagtggtaga tgccgaagaa gcagacgttc cccgcgaagg cgaagatcac cgcgttggtg
     3181 tgcagcggcc gcaatcggcc gaaggtcgtg aatgacagcc ccagattcgc gggccaccac
     3241 gcgagctcca gcgcgatcca gacgccgacg agcatgccca cgaccgccca cacgacggtc
     3301 gcggtcacga acatcttgac gatctggttg tcgtagcgag gctcgtcccc gttcctcacc
     3361 ccggtatatt ccatgacgtc gtggtcctcc tcggtgcggc gtttctcacc ggcttgctcg
     3421 gcagcgtgca ttgtgtcgcc atgtgcggcg ccttcgccgc ttcctgcact cgcgtgccgc
     3481 gcggtctccc cgcatggcac gccggacgca tcgggacgta cgcactgctc ggcgccctcg
     3541 ccggcgccgc ggggcggctc ctcccgggac cgccctggct gccgcccgcg gtcgccgcct
     3601 cgctgctgct gtggttcgcc ctcgccctcg ccggcgtggt ccccgaaccg cgtctcatcc
     3661 cgcggggact cacgaacgcc gccagccgtg ccgcacaaac gccctcgccc gcggcccaag
     3721 ccgccttcgg ggtcgcgaac ggcttcctgc cctgcggtct cgtgtactcg gcgctcagca
     3781 tccccgtcgc gctcgccgat ccggtgcgcg gcgcgctcgc catgatcgcg ttcggcaccg
     3841 gcacgctccc cgccctcagc ctcgccgccg tcggcctgcg gcgcctggtg atgacgagcc
     3901 tgtggcgcag gcgcgtgttc gccctgctcg tcctggtcgc cggagcctgg gcgatctgga
     3961 cgcgcgcgac cggcgccgct accgccggcc accaccacgc gccggcagca ccgccggccc
     4021 gccagaatcc gggttcagat taggcggcac tcagtgaagg acagcgctgt ggctggatga
     4081 agaaaagttc acgctcgcgg gcaagccgcg ccccggcgag cagttacgac gggccggtta
     4141 cttcaggccg gtgagctgga tgcctttgac gaagtatcgc tgggcgatga agaacaacac
     4201 cacgagcggc acgactgccg tcaccgccgc ggccatttga tacggccagt tcgtgtaata
     4261 gatgccgtgg aacgaagcga tcccgacttc gaccacctgc atacgcaccg agcgcgtgac
     4321 gaccagcggc cagaggaagc tcttccactg gtccaccagg ccgaacaccg ccagggtggc
     4381 cagggcggga cgggacagcg gcagcacgac cttccagaag atctgccact ccccggcgcc
     4441 gtcgagccgg gccgcgtcct ccagctcccg cggcagggtc tggaagaact gccgcagcag
     4501 gaagatcccc cacacggtga ccagctccgt cgtgatcaag cccgcataac tgtccaccca
     4561 gccgagcgtg ctcacgatca gaaaccgggg aatgagcagc acgacggtgg ggaccatcag
     4621 gacggcgaga aacagcagaa aaatgcggtc acgccccggg aaccggaggc gggcgaacgc
     4681 ataggccgcg gcggcgctgg tgagcacctg ccccgttacg acgacgatcg atacgaaggc
     4741 ggagttcacg aagaagcgcc agaacggcaa cgccgtgagg gccgcgccgt agttgtcgaa
     4801 tttcggatgc gcgggcagga tggccggggg atagcgcatc acctggaact catccatcag
     4861 cgacgtggtg agcatgtaaa ggaagggcag cgccatcgtc accccgacga tgccgaccag
     4921 ggacaggcgc gaccagcggc cccagcggat cctcttctgg cgcggggcgt tcgctgcgga
     4981 tccctcaggc atactggacc tgcttcccca gcagccggaa ctgcacccag gtggcgacga
     5041 acagcagcgc gaacagcagc agcgccaggg cgctcgccgg cccgaactgc aggaattccc
     5101 acgccagctg gtagatgcga tagaccagga cgtcggtcgc gtgcagcggg ccgccgtcgg
     5161 tgagcacgta caccaacgtg aacacctgga acgacgtgat caccccggtg acgagcacga
     5221 acagcgtgac gggccggagc aggggaaacg tgacccgcca gaagcgacgc caggcgttgg
     5281 cgccgtccac tcgcgccgca tcgaggtact cccgcgggat gccctgcagc ccggcctgga
     5341 acacgaccat ctggtagccc acctgcaccc agatcgagac gagcatgacg gcgagcagcg
     5401 cggtgcgcgg gttgcccagc caatcgaccg gccggatgcc gaccagcccg acgaaatagt
     5461 tcagcaggcc gaagtccggg tggtacatcc actgccagac cagcgcgatc gccacggcgg
     5521 acgagacgta cggcaggaag aacaccgtgc gcaccaggcg ggtggcacgc gaatggcggc
     5581 gcagcaggag ggccatgccc agcgcgatgg ccatggcgac cggcacgtgc agcgaataga
     5641 gcagcgtgtt ccggagcgaa gtccacacga gcggatcgcg caccaccgtg acgaagttcg
     5701 cgagccccac gaaggggcgg gccggctcga tgggactcca gcggtgcaac gcgaggtaca
     5761 gcgcgaagag catcggaccg aacgagaacg ccacgagatg cagcgtcgcg ggggcgagga
     5821 acccccaggc ggccagcgtc tcgcgcagcg cccgcgggcg cgcccgtgac cgctcgatcc
     5881 atccgagcag cagcgccccg agctgcccca tggcggcgag cgcgagcgtg agcacgagcc
     5941 agccgccgtc attgtccccg gcgaagtccc gcagcaccac ccaccggtcg aagccgagtc
     6001 gcacgcggat ttccgcccgg ggcacgcccg cgacggtcac aaggcgcacg tcggtcggcc
     6061 cgctgtccgc tacggcgagg tccgactcgg gagcgaggcg cgccagccga tcgagcggag
     6121 gccagacccg cacgggaaac cgcgcggccg cctcctcgag caacaggcca gtctggctca
     6181 gccagcgggt ggtcgcgtcc ttccgcccct gcaccgctt
//
LOCUS       DBNGZW010000176         5531 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1585605, whole genome shotgun sequence.
ACCESSION   DBNGZW010000176 DBNGZW010000000
VERSION     DBNGZW010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5531)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5531)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5531
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1585605"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..430
                     /locus_tag="AC16JC_04305"
     CDS             <1..430
                     /locus_tag="AC16JC_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163953.1"
                     /translation="LFRSALAAVLPILQACGSAGSGGQTPNPYTGPITRAELQDVGAR
                     DAYEAVQRLRPRWLVVRSMRSFSIETQVVVFQDKLYLGNQDELRRIGLEGIYSIEYLD
                     GATAQATLPGLRDLHVEGAIVIHMSPPDGGGDGGGAGSAG"
     gene            complement(427..876)
                     /locus_tag="AC16JC_04310"
     CDS             complement(427..876)
                     /locus_tag="AC16JC_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163954.1"
                     /translation="MSPKSLLRIRTGIVILAGAWAAACVDLNVTPQAPTLWDAQLTPA
                     LGYPDLAGQGAAVSQADGTNVGLAISGALPGAQHAWGLRLGTCAVPSQQIGLDAAYPV
                     LVVDSAGGASVQTHLGTSLAPGKRYHIAVRLSASDTTRVACGDLTER"
     gene            complement(873..1589)
                     /locus_tag="AC16JC_04315"
     CDS             complement(873..1589)
                     /locus_tag="AC16JC_04315"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR5163955.1"
                     /translation="MLDGHVSDDVTGQRLTGAQVLLLDRYSHTVGFTVTDDSGYFQFS
                     RSQNGWYRFEVKAIGYQKALTPMLRWLADHSYAEVEVRLAPHSVLLAPLEVVALSPAS
                     SSPVLENVAQRRTQGFGTQITREDIERRHPATVTDMLIEVPGVYAARRGTGASGRSIY
                     MGRSLYGPGGGPCPVQIFLDGRLATRNAPGGDVMIDDIVSPLDVEAIEIFKGLSSIPP
                     EFLTPDARCGVIAIWTKRAQ"
     gene            1778..2398
                     /locus_tag="AC16JC_04320"
     CDS             1778..2398
                     /locus_tag="AC16JC_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163956.1"
                     /translation="MRTRALITWCVTSISLLLPGAGLQAQAARREGPNLRTTSYLGLG
                     YVASVPITPLGFSLLVVSPKVFRGAGVYGDVKLTTSSAGSDPYYLPGVSVADAEITYG
                     DLLYEQRSDWLTIDLAMVYAITGEFAVYAGAGYSREKHFRQYFDDTQSRGDLGFYWIS
                     DPAESGTRVNVLGGALVRLRHFLYFQMGVESAPRAVNLGLTVTVAR"
     gene            2837..>5531
                     /locus_tag="AC16JC_04325"
     CDS             2837..>5531
                     /locus_tag="AC16JC_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR5163957.1"
                     /translation="MRFTSGTIRLGALLAAALLAGAASTGRAQQGTIAGLVTDQLNNA
                     PVAGARILLGNTNRTAVTNTSGRYAFTGVPVGSYELRVVAVGFRAQLKVVPVEAGGSA
                     TADFALTRAVISLDEIVVNPVTGVQRARETGNAVTNIDLPKALENQTVSNFADALSGR
                     AAGVQVLQSGGTVGTGTRIRIRGQSSLSLSNEPVYYIDGVRVESGDNSLSVGTGGQAP
                     SRVNDINPEDIANVEIVKGPSASTLYGTQAANGVVRITTKHGLAGRARWRIYSEGGML
                     SDKNQYPTNYFSWGHLTPDTFPAARRIVQCTLVASQRTTGTSGSCAIDSLTSYNVLMD
                     PKQTPIGTGYRGQGGLQVQGGSDQVQYYVSGDYQDELGVYRLPGVEYARLTTASGGIA
                     PDYQVYRPNELRATSLRSNVHVQPMAALDFTGNLGILQSRGRLPQNDNNVTGFLPSGL
                     FGRGQAGSAGIWGFFLPGDVFQILVQQDITRITGSFAGNWRPTTWFTARGNVGVDYTG
                     RTDINFQLRGQGTNFSNFRQGRRSDNRFNISHYTGDIGGTAAFTLAPELNSKTSVGFQ
                     YLKDNFFGVLATGTILPPGGKAITGAAQRTATEQTTVAVTLGTYLEQTFGWRDRVFLT
                     AGVRNDRNSAFGSKERSVVYPKVQGSWVLSDESFYPRSLPISNLKLRFAYGASGQQPG
                     TTDAILFYAAQTATLLAGGTGTATDQPGIDLTAFGNTSLKPERSTEFETGFDAGLFHD
                     AARLEVTYYNKRTRDALINRTLPPSNGIAQNRFENIGSVQNRGLEAQLSVTKNVTPGI
                     GVDAALSFARNSNKLLSLGAGIPPVVNGNVREVPGYPLFGFWDRPITGFNDADGNGII
                     TANEVTVGPTPVFLGSSIPKTTITLNAGITLFRNKVRLGGQL"
BASE COUNT          922 a         1846 c         1885 g          878 t
ORIGIN      
        1 gctcttccga tctgcgttgg ccgccgtgct cccgatcctc caggcttgcg gatccgcggg
       61 atcagggggc cagaccccga acccctacac ggggccgatc acccgcgccg agctccagga
      121 cgtcggcgcg cgcgacgcct atgaggcggt gcagcgcctc cgtccccgct ggctcgtcgt
      181 gcgaagcatg cgcagtttct ccatcgaaac ccaggtggtg gtgttccagg acaagctgta
      241 tctcggcaac caggacgagc tgcggcggat cgggctcgag ggcatctatt ccatcgaata
      301 cctcgatggc gcgacggccc aggcgaccct gccggggctc cgggacctgc acgtcgaggg
      361 cgcaatcgtc atccacatga gccccccgga cggggggggg gatggtgggg gagcagggtc
      421 cgcgggctaa cgctccgtca gatcgccgca ggccacccgg gtggtgtccg aggcgctcag
      481 gcggaccgcg atatgatacc gctttcccgg cgcgaggctg gtgccgagat gcgtctggac
      541 cgaggcgccg ccggcggagt cgacgaccag cacgggatat gcggcgtcga ggccgatttg
      601 ctggcttggc actgcgcagg tgccgagccg cagtccccac gcatgctgtg cccccggcag
      661 cgcgccgctg atggcaagcc ccacattcgt tccgtcggct tgtgacacgg cggctccctg
      721 accggcgagg tccgggtagc ccagggccgg cgtgagctgc gcgtcccaca gggtgggcgc
      781 ctgcggggtg acgttgaggt cgacgcaggc cgccgcccac gcaccggcca ggatcacgat
      841 tccggttctg atccggagca gggatttcgg gctcactgcg cgcgtttcgt ccagatggcg
      901 atcacgccgc agcgcgcatc cggggtgagg aactccggcg ggatgctgct gagtcccttg
      961 aagatctcga tcgcctcgac atcgagcggt gacacgatgt catcgatcat gacgtcgcct
     1021 ccgggcgcgt tgcgggtggc gagccggccg tcgaggaata tctgcaccgg acaggggccg
     1081 ccgccggggc cgtagagcga ccggcccata tagatggatc ggccgctggc gccggtgccg
     1141 cgccgcgctg catacactcc cgggacctcg atcagcatgt ccgtgacggt cgcggggtgg
     1201 cgccgctcga tatcctcccg cgtgatctgt gtgccgaatc cctgcgtccg gcgctgcgcg
     1261 acgttctcca gcaccgggct gctcgacgcg ggggagagcg caacgacttc aaggggcgcc
     1321 agcagcaccg agtgcggcgc gagccggacc tctacttccg cgtacgagtg gtccgccagc
     1381 cagcgcagca tgggggtgag ggctttctgg tagcctatcg ccttgacctc gaaccgatac
     1441 cacccgtttt gggaccggga gaactggaag tagccgctat cgtcggtcac ggtgaacccg
     1501 accgtgtggc tatatcggtc gagcagcaga acttgggcgc cggtgaggcg ctgcccggtc
     1561 acgtcatccg acacgtgccc gtcgagcacc acctgagcac gtgcaggcgt ggcggtgcat
     1621 atcgccagaa ggacagccgg cacggcgaag gtagccacac ggcttcgggc atgcatattt
     1681 ggcccacccg ggatggctta agctcaggag aacgctggtt gccaagctta ggggttccgc
     1741 agagtctccg caagtccgcg aaggagggac gaggaggatg agaacccgtg cgctgatcac
     1801 gtggtgcgtg acgtccatca gcctcctgct tccgggcgcg ggcctgcagg cgcaggcggc
     1861 tcgccgcgag gggcccaacc tgcggaccac ctcctacctt gggctcggct atgtcgcgag
     1921 cgtacccatt acgcccctgg ggttttccct gctggtggtc tcaccgaagg tcttccgcgg
     1981 ggcgggggta tacggcgacg tcaagctcac gacctcctct gccggatccg atccctacta
     2041 cctcccgggc gtctccgtgg cggacgccga gatcacctac ggcgatctgc tctacgagca
     2101 gcggagcgac tggctcacca ttgatctggc catggtgtac gcgatcaccg gcgaatttgc
     2161 cgtgtacgct ggcgcggggt actcgaggga gaagcacttc cgccagtatt tcgacgatac
     2221 acagagtcgc ggtgacctgg ggttctactg gatctcggac ccggcagagt cggggacgcg
     2281 tgtaaacgtt ctgggggggg cgctcgttcg cctcaggcac ttcctctatt tccagatggg
     2341 ggtggagtca gccccgcgcg cggtgaacct cggccttacc gtgacggtcg cgcgttgagg
     2401 cagggggtcc ctcttgtggc ctcggcggat ttggtgccag aattaacctg atggcgccgc
     2461 cggctccccg tgcggcgccg tgccatcaca cgaccacacg acgccgtcgc gggctaccga
     2521 cactcacctc aagccgttgg gtggatcgca gtcccgagac catgatgatt cgatgaaacg
     2581 ggtctgaagg tgcaggcccc gggggtcgcc ggcctaacag gccgggccgt ccggcgttgg
     2641 cgttctcact cggtgaaagg aggacgacac atggggtttc gcgctgtccg gctgagttcg
     2701 gctctctgct gctacggctg ctcacattga cgggaggcgt gcgcccgggt accggcgtgc
     2761 gacctcccga ttccgcatcc gaagtgcgac agcttgagac acggagcact cgaaacgaac
     2821 tcgaggagaa acgaccatgc gatttaccag tggtaccatc aggcttggcg cgctgctggc
     2881 cgccgcgttg ctcgccggag cggcgagcac gggcagggcc cagcagggca ccatcgcggg
     2941 cctggtcacc gatcagctga acaacgctcc ggtggcgggc gcgcgcatcc tgctcggcaa
     3001 cacgaaccgc acggcggtca ccaacacgag cggccgctac gcgttcaccg gcgttcccgt
     3061 cggctcctac gagctgcggg tcgtggccgt gggctttcgg gcacagctga aggtcgtccc
     3121 ggtggaagcg ggcggctcgg ccaccgccga tttcgcgctg acccgagccg tgatctcgct
     3181 cgacgagatc gtggtgaacc cggtgaccgg cgtgcagcgt gcccgtgaaa cgggcaacgc
     3241 ggtcacgaac atcgatctgc ccaaggcgct cgagaaccag accgtctcga acttcgccga
     3301 tgcgttgtcc ggccgcgccg caggggtcca ggtgctgcag tcgggcggca ccgtgggcac
     3361 gggaacccgg atccgtatcc gggggcagag cagcctgtcg ctgtcgaacg agcccgtcta
     3421 ctacatcgac ggggtccgcg tcgagagcgg ggacaactcg ctgtcggtgg gcacgggcgg
     3481 ccaggcgccg tcgcgcgtca acgacatcaa tcccgaggac atcgccaacg tcgagatcgt
     3541 caagggcccg tcggccagta cgctgtacgg gacacaggcg gccaacggcg tcgtgcgcat
     3601 caccaccaag catggcctcg cggggcgtgc acgctggcgg atctacagcg agggcgggat
     3661 gctgagcgac aagaaccagt atcccacgaa ctacttcagc tgggggcatc tcacacccga
     3721 taccttcccg gccgcccggc gcatcgtgca gtgcacgctc gtggcctcgc agcggaccac
     3781 cggcacgtcc ggctcgtgcg ccatcgacag cctgacctcc tataacgtgc tcatggatcc
     3841 gaagcagacg cccatcggca ccggctatcg cgggcagggc ggcctgcagg tccagggcgg
     3901 cagcgaccag gtccagtact acgtctcggg cgactatcag gacgagctcg gcgtgtaccg
     3961 gctgcccggg gtggagtatg cccgtctcac gacggcgtcg ggcggcatcg caccggatta
     4021 ccaggtgtac cgtcccaacg agctgcgggc gacgagcctg cgctccaacg tccacgtgca
     4081 gccgatggcc gcactcgatt tcacgggcaa cctcggcatc ctgcagagcc gcgggcggct
     4141 gccgcagaac gacaacaacg tcaccgggtt cctgccgagc gggctgttcg gccgcgggca
     4201 ggcaggctcg gcgggcatct ggggcttttt cctccccggc gacgtcttcc agatcctggt
     4261 gcagcaggac atcacccgga tcacggggag cttcgcgggc aattggcgtc ccaccacgtg
     4321 gttcaccgcc cgcggcaatg tgggcgtgga ctacacgggg cgcaccgaca tcaacttcca
     4381 gctgcgcggc caggggacga atttctcgaa cttccgccag ggccgccgca gcgataaccg
     4441 gttcaacatc tcgcactaca ccggggacat cggcggcacg gccgcgttca cgctcgcccc
     4501 ggagctcaac tcgaagacgt cggtggggtt ccagtacctg aaggacaact tcttcggcgt
     4561 gctggccacc gggaccattc tgccgcccgg agggaaggcg atcaccggcg ccgcgcagcg
     4621 gacggcgacc gagcagacca ccgtcgcggt gacgctcggc acctacctcg agcagacgtt
     4681 cggctggcgc gaccgggtgt tcctcaccgc cggggtgcgc aacgaccgta acagcgcgtt
     4741 cggcagcaag gagcgctcgg tcgtgtatcc caaggtgcag gggtcgtggg tcttgtcgga
     4801 cgaatcgttc tacccccgca gcctgccgat cagcaacctg aagctgcggt tcgcctacgg
     4861 ggcgtcgggc cagcagccgg gcacgaccga tgcgatcctg ttctacgcgg cgcagaccgc
     4921 gacgctgctg gcgggcggaa cggggacggc gacggaccag ccggggatcg acctcaccgc
     4981 gttcggcaac acgtcgctga agccggagcg atccacggag ttcgagaccg gattcgatgc
     5041 cggcctgttc catgacgcgg cgcggctcga ggtcacctac tacaacaagc ggacgcgcga
     5101 cgccctcatc aaccggacgc tgccgccgtc caacggcatc gcccagaacc gcttcgagaa
     5161 catcggctcg gtacagaacc ggggcctcga agctcagctc agcgtgacga agaacgtgac
     5221 gcccggcatc ggcgtggatg cggcgctgag cttcgcccgc aatagcaaca aactgctgtc
     5281 cctcggtgcg ggcatccccc cggtcgtcaa cgggaatgtg cgggaagtac cggggtaccc
     5341 gttgttcggg ttctgggatc ggccgatcac cggcttcaac gacgccgacg gcaacggcat
     5401 catcacggcg aacgaagtca ccgtggggcc gacccccgtg ttcctcgggt cgtcgattcc
     5461 gaagaccacg atcaccctga acgccgggat caccctgttc cgcaacaagg tccgtctcgg
     5521 cgggcagctg g
//
LOCUS       DBNGZW010000177         2916 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_768013, whole genome shotgun sequence.
ACCESSION   DBNGZW010000177 DBNGZW010000000
VERSION     DBNGZW010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2916)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2916)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2916
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_768013"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..249)
                     /locus_tag="AC16JC_04330"
     CDS             complement(<1..249)
                     /locus_tag="AC16JC_04330"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR5163958.1"
                     /translation="MNHALALLALVLDVIVRAWRIQLAAWTAGGALALWPAVRLNLYG
                     EAASTLTPNRLGGEAARFAGLTQAGLRSVTALVAVGVEV"
     gene            complement(246..533)
                     /locus_tag="AC16JC_04335"
     CDS             complement(246..533)
                     /locus_tag="AC16JC_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012339.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR5163959.1"
                     /translation="MTVTFLPDRRSVVIRPGATVLQLSAATGDPLRTVCGGIGNCTAC
                     RVRVVEGEWPAGKTDRLRLGPLVEQGWRLACQFSPRQTVTLERPPYTLDGL"
     gene            complement(656..1294)
                     /locus_tag="AC16JC_04340"
     CDS             complement(656..1294)
                     /locus_tag="AC16JC_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019588787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5163960.1"
                     /translation="MKVVQIPNGVFAENCYLVIDEAAAECAVVDPGEEAGLILHKIEQ
                     EGVRAGGIWVTHAHLDHVMGVARVRAATGAPVWLHAADRPLYDRVSQQGVAFGLPAEQ
                     PPPPDASWSHGDVARVGRLTFSVRHTPGHSPGSVALVGEGVVFSGDVLFQGSIGRTDL
                     PGGDFATLIGSIERELLVLPDATIVYSGHGPQTTIGAERRSNPFLTGAVPIA"
     gene            complement(1291..2274)
                     /gene="trxB"
                     /locus_tag="AC16JC_04345"
     CDS             complement(1291..2274)
                     /gene="trxB"
                     /locus_tag="AC16JC_04345"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_391359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR5163961.1"
                     /translation="MPENGQQSYDVIIVGAGPAGLCAGMYAGRGMLKALTIERGAPGG
                     ELLNTDLVEDYIGFESIKGWDLAQKFTDHAKKFGAEIVTDTVESVRRAPDGWFDVTTA
                     RERHYRAPAVILTAGGTPVKLGVPGEREYAGKGVSYCAVCDGAFFKGEVLAVVGGGDA
                     AVEEADYLTRYASKVYIVHRRNRFRASKILQERAFANPKIEAVWNKRVVEIAGTERGT
                     DHLELEDTATGARSQLKVGGVFIFVGFKPNTGIVEGHVDHDAGGYFVTDERMMTSIPG
                     LFAAGDVRSQLTRQVTTAVGDATTAAIAVEKYLAQLREQGRLSASAGAIVT"
     gene            complement(2267..2626)
                     /locus_tag="AC16JC_04350"
     CDS             complement(2267..2626)
                     /locus_tag="AC16JC_04350"
                     /inference="COORDINATES: protein motif:HMM:NF015690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HIR5163962.1"
                     /translation="MASLLDPQARRRQLEQAQALFNDGEYWLAHEALETVWRSIINDG
                     ERRIWQGLIQAAAALLHRRRGNRHGTVVVGAAALEKLSGPQHPAVEFDTLRFRAALAR
                     ALAGEGDPPRLDFRSDA"
     gene            complement(2626..>2916)
                     /locus_tag="AC16JC_04355"
     CDS             complement(2626..>2916)
                     /locus_tag="AC16JC_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163963.1"
                     /translation="CPAIGGPRSWEMSEAVQALAERLRSRGESIAAPVLFVCRHHVFG
                     VGTFAVDAVLEGERIVGAAAAAPGTAQVLVGTVSACHGALNLLRIGPSPADG"
BASE COUNT          461 a         1089 c          937 g          429 t
ORIGIN      
        1 gacttccacg ccgaccgcga ccagcgccgt caccgagcgc aggccggcct gggtcagccc
       61 cgcgaaccgc gccgcttccc cacccagccg gttgggcgtc agggtggagg cggcttcacc
      121 gtagaggttg agccgcacgg cgggccagag ggccagcgcg ccgcccgccg tccaggccgc
      181 gagctggatg cgccaggcgc ggacaatgac gtcgagcacg agcgcgagca aggcgagggc
      241 gtggttcaca ggccgtccag cgtgtacggc ggccgctcga gcgtcaccgt ctgacgcggg
      301 ctgaactggc acgcgagccg ccacccctgc tccaccagcg gccccaaccg gagccgatcg
      361 gtcttgccgg cgggccattc tccttccacc acacggacgc ggcaggccgt gcagttgccg
      421 atgccgccgc acacggtccg cagcggatcg cccgtggccg ccgacagctg cagcacggtg
      481 gcccccggac ggatcaccac actccggcgg tcgggcagga aggtgacggt cacctcaatc
      541 agagcagtcg cccagcggat agatgaagcc gcagttggcg cacggattct tgcaccccca
      601 cggcttgggg cgcacctccg cgccgcaccg caggcacgtg ggccgcggcc gactcctagg
      661 cgatggggac ggcgccggtg aggaagggat tggaacggcg ctcggcgccg atggtggtct
      721 gtggtccatg cccgctgtac acgatggtcg catcgggcag cacgagcaac tcccgctcga
      781 tgctcccgat caacgtcgcg aagtcgccgc cgggaaggtc ggtgcgcccg atggagccct
      841 ggaacaacac atcgccgctg aagacgacac cctcacccac gagcgccacg ctcccgggcg
      901 aatgaccggg cgtgtgccgc acgctgaagg tgaggcggcc gacccgcgcg acgtcgccgt
      961 gtgaccagga ggcgtcggga ggcgggggtt gctcggccgg gaggccaaat gccaccccct
     1021 gctgcgacac acggtcatac agcggccggt cggctgcatg cagccacacc ggagcaccgg
     1081 tggcggcgcg cacccgtgcg acgcccatca cgtgatccag atgcgcgtgt gtgacccaga
     1141 tcccgcccgc ccgcacccct tcctgttcga tcttgtggag gatcaatccc gcctcttcac
     1201 cggggtccac gacggcgcac tcggccgccg cctcgtcgat caccaggtag cagttctcgg
     1261 cgaatacccc gttggggatc tggaccactt tcacgtgacg atggctcctg cggaagcgga
     1321 cagcctgccc tgctcccgca gctgcgccag atacttctcc actgcgatcg cggccgtggt
     1381 cgcgtccccg actgcggtgg tgacctgccg cgtcagctgg ctgcgcacgt cgcccgccgc
     1441 gaacaaccct gggatcgagg tcatcatgcg ctcgtcggtg acgaagtacc cgccggcgtc
     1501 gtggtcgacg tggccctcca cgattcccgt attcggtttg aacccgacga agatgaacac
     1561 cccgcccacc ttgagttggc tgcgggctcc cgtcgccgtg tcttcgagct ccagatggtc
     1621 ggtgccacgc tcggtccccg cgatctccac gacccgcttg ttccagaccg cctcgatctt
     1681 cggattcgcg aaggcccgtt cctgcaggat cttcgatgcc cggaaccggt tgcggcggtg
     1741 cacgatgtac accttcgaag cgtagcgtgt gaggtagtcc gcctcttcga ccgcggcgtc
     1801 gccgccgccc accaccgcca gcacctcgcc cttgaagaag gcgccgtcgc acacggcgca
     1861 gtaggaaact cccttgccgg cgtactcccg ctcccccggc acgccgagct tcaccggcgt
     1921 gccgccggcc gtgaggatca ccgcgggagc gcggtagtgc cgctcgcgcg cggtggtcac
     1981 atcaaaccac ccgtccggcg cacgccgcac gctctcgacg gtgtcggtga cgatctcggc
     2041 gccgaacttc ttggcgtggt ccgtgaactt ctgggccagg tcccacccct tgatgctctc
     2101 gaacccgatg taatcttcga ccagatcggt attcaggagc tcaccaccgg gagcgccccg
     2161 ctcgatggtc agggccttga gcatcccccg gccggcatac attcccgcgc acagccccgc
     2221 cggaccggcg cccacgatga tcacatcgta gctctgctgc ccgttctcag gcatcgctcc
     2281 gaaagtccaa tcgcggggga tctccctccc ccgccagggc acgcgccagg gcggcgcgaa
     2341 accgcaacgt atcgaactcc actgcggggt gctgcggccc ggacaacttc tcgagggccg
     2401 cggccccgac gacgacggtg ccgtgacggt tgccgcgccg ccggtgcagc aacgccgcgg
     2461 ccgcctggat caacccctgc cagatccgcc gctcaccgtc gtttattata gaacgccaga
     2521 cggtttccag ggcttcgtgg gcgagccagt attcgccgtc gttgaacagc gcctgggctt
     2581 gttccagctg gcgccggcga gcctgcggat ccagcaaaga agccatcagc cgtcagcagg
     2641 cgacggcccg atccgcagca gattcaacgc gccgtggcag gcgctcaccg tgcccaccag
     2701 gacctgggcc gttcccgggg ctgccgcggc tgcgccgacg atccgctccc cctccaggac
     2761 tgcgtccacg gcgaacgtcc ccacgccgaa cacgtggtgg cgacacacga acaggacggg
     2821 cgccgcgatg gactcacctc tcgaccgcaa tcgttcggcc agcgcctgca ccgcctcgga
     2881 catctcccac gaccggggcc cgccgatcgc cgggca
//
LOCUS       DBNGZW010000178         2861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1311691, whole genome shotgun sequence.
ACCESSION   DBNGZW010000178 DBNGZW010000000
VERSION     DBNGZW010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1311691"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /locus_tag="AC16JC_04360"
     CDS             complement(<1..1115)
                     /locus_tag="AC16JC_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163964.1"
                     /translation="MKPSSGQWMARALRGLGVIACVVAVWRGGPWGLVAVGGGFMLAY
                     GFRMARVVRTWLHEQAPVTPSDLAHTLDLLRRAHDATAAWAVGLDEQEVEAYGDPKPP
                     VPVRERGGAIVQLASVDGRSHVAREDEGTYVAVGDFPYGAGLLLTERDADPEVAQSVT
                     EELRRIVAAMRLSELEVPEGQFQLVAKQLAMIAAGAQTLEGVAKAGAELAQQLAQRGT
                     AVVILDPTTQAAQVLAVSTAADRRLAGLTLSSEAPAHRAMQTGLPVVTQSSEDIFGPG
                     VPERRRQERAGQAYPLMDGHFAIGALVLLGPAILPESPLADQVGQLVSELGPRLAAAR
                     AVHEAEQRAVVDPLTGLRNRREFERQIESFKAKQQERG"
     gene            complement(1112..1798)
                     /gene="larB"
                     /locus_tag="AC16JC_04365"
     CDS             complement(1112..1798)
                     /gene="larB"
                     /locus_tag="AC16JC_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007778226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HIR5163965.1"
                     /translation="MRSGDHGPPFDDLPFVTIDHLRPVTQGHQEVIFSQGKTVEQVVA
                     IAERLVLAGGTFLATRTEAAQRAALVGRFPEALVNDLGRTVYLPPAPEPPPTGRGTVL
                     VVTAGTADLAVAEEAAVTARALRNRVERLTDVGVAGIHRVLSQTSLLRSATVIIVVAG
                     MEGALPSVVGGLVKVPVIAVPTSVGYGASFGGLAALLGMLNSCAAGVTVVNIDNGFGA
                     AAAASRINHL"
     gene            complement(1795..2802)
                     /gene="dusB"
                     /locus_tag="AC16JC_04370"
     CDS             complement(1795..2802)
                     /gene="dusB"
                     /locus_tag="AC16JC_04370"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR5163966.1"
                     /translation="MKFPFQVPHDVALFLAPMAGVSEAPFRRIARAFGADVVVSEFIS
                     SVGVSLGIERVLAEMRFEDVERPIGIQLYGADPATMARAATLVTELFEPDYLDINFGC
                     PVKKVVRNNGGSGCLKDMGLVGGIIRAVREATPLPVTAKIRSGWDEAQRDPVTIALRC
                     QDAGAQALALHPRTRTQMYNGAADWDEIARVVEALDIPVIGNGDIRTPEDVLAMRRHT
                     DCAGIMVARGSFGNPWIFSQGRDLLEGRSMRPAPTAQERFAVALQHARLALRLQGDTR
                     KTALEFRKHLGWYTKGLPGSAELRQRLFAIESMHEAERLFASYLAEHPVADAAPVGAA
                     A"
BASE COUNT          438 a         1093 c          931 g          399 t
ORIGIN      
        1 ccccgctcct gctgcttcgc cttgaacgat tcgatctggc gctcgaactc gcgccggttc
       61 cgcaacccgg tcaacgggtc caccaccgcc cgctgctcgg cctcgtgcac ggcgcgggcg
      121 gccgccagcc gggggccgag ctcggacacg agctggccca cctgatcggc aagcggtgac
      181 tccggcagga tcgcgggacc cagcaggacc agggcgccga tcgcgaagtg gccgtccatc
      241 aggggatacg cctgaccggc gcgctcctgg cgccgtcgct cgggaacgcc cgggccgaag
      301 atgtcttcgc tcgactgcgt cacgaccgga agaccggtct gcatggcccg gtgcgccggt
      361 gcctcggacg agagcgtcag tccggccagc cggcggtcgg ccgccgtcga caccgccaac
      421 acctgcgccg cctgcgtggt gggatccagg atcacgacgg ccgtgccgcg ttgcgccagc
      481 tgctgcgcca gctcggcacc cgccttcgcg acgccttcca gcgtctgggc accggcggcg
      541 atcatggcga gctgtttggc gacgagctgg aactgtccct ccggcacctc gagctccgac
      601 agccgcatcg ccgccacgat gcggcgcagc tcctcggtga ccgactgggc cacctcgggg
      661 tcggcgtcgc gctcggtgag cagcaggccc gccccgtagg ggaaatcgcc caccgccacg
      721 taggtccctt cgtcttcccg cgccacgtgg gagcgcccgt ccacggatgc gagctgcacg
      781 atcgcgccgc cgcgctcgcg gacgggcact ggtggcttgg gatcaccgta ggcttccact
      841 tcctgctcgt cgaggcccac cgcccacgcc gcggtcgcgt cgtgcgcccg gcgcagcaga
      901 tcgagggtgt gcgccaggtc cgacggcgtc accggcgcct gctcgtgcag ccaggtccgc
      961 accacgcgcg ccatccggaa cccgtaggcg agcatgaatc cgccgcccac cgcgaccagt
     1021 ccccagggac cgccgcgcca caccgccacg acgcaggcga tcacgcccag cccgcgcaat
     1081 gcgcgcgcca tccattgccc gctcgagggc ttcacaggtg gttgatccga ctggcggccg
     1141 ccgccgcgcc gaacccgtta tcgatattga cgacggtcac gccggcggcg cacgaattca
     1201 gcatgcccag cagtgcggcc aggccgccga acgacgcgcc gtatccgacg ctcgtcggca
     1261 ccgcgatcac cggcaccttc acgagtccgc cgaccacgct cgggagcgcg ccttccattc
     1321 cggcgacgac gatgatcacc gtggcgctgc gcaggaggct cgtctgactc aagacccgat
     1381 ggatgccggc caccccgaca tcggtgagcc gctcgacacg gttccgcaac gcccgtgccg
     1441 tcaccgccgc ctcttcggcg actgcgaggt cggccgtccc cgcggtcacc acgagcaccg
     1501 tcccccgccc cgtcggcggc ggctccggcg ccggcggcag gtacacggtg cgccccaggt
     1561 cgttcaccag cgcctccgga aagcgcccga ccagagcggc gcgctgcgcc gcctcggtgc
     1621 gggtcgcaag gaacgtgcct cccgccagga cgagccgctc ggcaatggcg acgacctgct
     1681 cgacggtctt gccctgcgaa aaaatcacct cctgatggcc ctgcgttacc ggccggaggt
     1741 gatcgatggt gacgaatggc agatcatcga acggcggccc atgatccccc gatctcatgc
     1801 cgccgctccg accggcgccg cgtccgcgac cggatgctcc gccagatagg atgcgaacag
     1861 acgctccgcc tcgtgcatcg actcgatcgc gaagagccgc tgccgcagct ccgccgaccc
     1921 gggcagaccc ttggtgtacc accccaggtg tttgcggaac tcaagcgccg tcttgcgggt
     1981 gtctccctgg agccggagcg ccagccgcgc atgctgcagc gccacggcga agcgctcctg
     2041 cgcggtgggc gccgggcgca tcgagcggcc ctcgagcaga tcgcgcccct gggagaagat
     2101 ccacggattg ccgaacgacc cgcgcgcgac catgattccg gcgcagtcgg tgtggcgccg
     2161 catcgcaagc acgtcctcgg gcgtgcgaat gtcgccgttg ccgatcacgg ggatgtcgag
     2221 cgcctcgacc acacgggcga tctcgtccca atcggcggcg ccgttgtaca tctgggtccg
     2281 cgtccgggga tgcagcgcca gcgcctgcgc tccggcatcc tgacagcgca aggcgatggt
     2341 gaccggatcg cgctgcgctt cgtcccatcc gctccggatc ttcgccgtga ccggcaacgg
     2401 cgtcgcctcc cggacggcgc ggatgatccc gccgaccagt cccatatcct tgagacagcc
     2461 cgacccgccg ttgttacgca ccactttctt gaccggacaa ccgaagttga tgtccaggta
     2521 gtccggctcg aacagctcgg tgaccagcgt cgcggcgcgc gccatggtgg cggggtccgc
     2581 gccgtacagc tggatcccga tgggtcgctc gacgtcctcg aaccgcatct ccgccagcac
     2641 ccgctcgatc cccagcgaca ctccgaccga ggagatgaac tccgacacga ccacgtccgc
     2701 gccgaacgcc cgggcgatgc ggcggaacgg cgcctcgctc accccggcca tcggggcgag
     2761 aaagagcgcg acgtcgtgcg ggacctgaaa cgggaacttc atgcgcacca acgtaatagc
     2821 gcgttgcgcg gacagcaacc ggccattacg ttagcctccg a
//
LOCUS       DBNGZW010000179         1751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_902601, whole genome shotgun sequence.
ACCESSION   DBNGZW010000179 DBNGZW010000000
VERSION     DBNGZW010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1751
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_902601"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            236..>1751
                     /locus_tag="AC16JC_04375"
     CDS             236..>1751
                     /locus_tag="AC16JC_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR5163967.1"
                     /translation="MMNIELQHTNEAIISRYTDQPVRLPVALRRAIEERWDGAPVQLY
                     ALADLDQSLSLAETWVALGPSHLATARRSGEGWIIESVWRSRVEAVREAPGLSASTLT
                     VLGRPGDPALLVVRYTHRQRRAFENLRFVLEEAIAGRAVASPDADREYADGVARPVRD
                     AQALVAGQRVAVIRRLLGYLRPYRSRLALGMGAAAVITLVSLVPPYLAGYLIDRVVRP
                     AQQGSLPLARASLIAWVAVGAMALVYLVRQAAALVRLRLMAILGEWVARDLRDELYEH
                     IQRLSLAFFSRKKTGSLITRVSADTDRLWEFLAFGVVDVSLSLVMLAGLSAVLLSLDW
                     RLGLVMTLPVPLFCWLIYRHGESINRLFIRAWRKWSRVSDVLSDTIPGIRVVKAFNQE
                     DREIARFGERNEDVTAEFNRIHHLWTGFWPLLMLGVHTMKVLVWVFAVPRLLGAGAAL
                     GAGTFVSFLLYTTMFVAPIEVIGQMARTMNRATTSAHRVFEVLDTEPEVRDVPQA"
BASE COUNT          233 a          554 c          661 g          303 t
ORIGIN      
        1 tctcgagcat gtacgcgatg aataccaccc cactgcagct ccgcctgcgg gcgttgcccg
       61 gcttcgtgtg ccggtccgcg ccgctcgccg gagtcggtgg gcgtattgcg tccagcgacg
      121 ccagctcgag gagacgagca ccctagaagt ccgcgattcc attgcgtgat ccggccttcc
      181 gggtgctccc agagacccgg aaggcctttc tgttgggcat gaacagagga tcacgatgat
      241 gaacatcgag ctgcagcaca ccaacgaagc gatcatctcc cgttacaccg accagcccgt
      301 ccggttgccg gtggcgttgc ggcgcgccat cgaggagcgc tgggacggcg cgccggtgca
      361 gctctacgcc ctggcggatc tcgatcaatc gctgtccctg gcggagacct gggtggccct
      421 ggggccgagc cacctggcca cggcacggcg gagcggtgag ggctggatca tcgagagcgt
      481 ctggcgctcc cgtgtggaag cggtccgcga ggcgccgggt ctgtcggcct cgaccctcac
      541 ggtcctcggg cgtccgggcg atccggcgct gctcgtggtg cgctacacgc accgtcagcg
      601 gcgcgcgttc gagaacctgc gcttcgtgct cgaggaagcg atcgcgggac gggccgtcgc
      661 gtccccggat gcggaccggg agtacgccga cggggtcgca cgccccgtcc gcgacgccca
      721 ggccctggtg gccggccagc gggtggccgt gatccggcgg ctgctggggt acctccggcc
      781 ctaccggagc cggttggcgc tcggcatggg tgcggcggcg gtcatcacgc tggtgagtct
      841 cgtgccgccg tatctcgcgg ggtacctgat cgaccgcgtg gtccggccgg cgcagcaggg
      901 gagcctgccg ctcgcgcggg ccagtctcat cgcctgggtc gcggtggggg cgatggcgct
      961 ggtgtacctg gtgcgccagg cggcggcgct ggtgcggctg cgcctgatgg cgatcctggg
     1021 cgagtgggtg gcgcgcgacc tgcgcgacga gctgtacgag cacattcagc gcctgtcgct
     1081 ggcgttcttc tcacgcaaga agacggggag tctgatcacg cgggtgtcgg ccgacaccga
     1141 ccggctgtgg gagttcctgg cgttcggggt ggtggacgtg tcgctgtcgc tcgtaatgct
     1201 ggcggggctc tcggccgtac tgctgtcgct cgactggcgg ctgggactcg tgatgacgct
     1261 gccggtgccg ctgttctgct ggctgatcta ccggcacggg gagtcgatca accggctgtt
     1321 catccgggca tggcgcaagt ggtcgcgcgt gtcggacgtg ctgtcggaca cgattcccgg
     1381 catccgggtg gtgaaggcgt tcaaccagga ggaccgcgag atcgcccgct tcggcgagcg
     1441 caacgaggac gtgacggcgg agttcaaccg catccaccac ctgtggaccg gattctggcc
     1501 gttgctgatg ctcggggtgc acacgatgaa ggtgctggtg tgggtgttcg cggtaccgcg
     1561 gctgctcggg gcgggggcgg cgctcggagc gggaacgttc gtgtcgttcc tgctctacac
     1621 cacaatgttc gtggcgccga tcgaggtcat cggccagatg gcgcggacga tgaaccgggc
     1681 gacgacctcg gcgcaccgcg tattcgaggt gctggacacc gagcccgagg tgcgggatgt
     1741 accgcaggcg g
//
LOCUS       DBNGZW010000180         3436 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_16793, whole genome shotgun sequence.
ACCESSION   DBNGZW010000180 DBNGZW010000000
VERSION     DBNGZW010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3436)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3436)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3436
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_16793"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="AC16JC_04380"
     CDS             complement(<1..1271)
                     /locus_tag="AC16JC_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR5163968.1"
                     /translation="MPQHLKPYANVLETIGWTPLIRLQRVMAGIRTPVYAKAEQFNPG
                     GSVKDRIGLAMVEEAEREGRLKPGGVIVEATSGNTGVGLALTAAVKGYRCIFTMPDKM
                     SQEKARLLRALGAEVIITPTAVAPDHPDSYIMKGRAIAASLPNAIFADQHYNPVNPEA
                     HYRTTGPELWAQTEGRITHFVCAPGTGGTVSGTARFLKERNPNIRVIAGDPVGSIYRE
                     YAASHRKAEGAPYKVEGIGGDKIPTSLHFDLIDEWIVVSDRDAMQMTRRLAREEGLLC
                     GGSTGVNLVSALDVARRLDDPEACVVTVLNDSGERYLSKVYNDEWLRENQLLEAERRT
                     VADLVAQKDHGAPPLVSVGSQATVRQALNLMSTFNVSQLPVLDGADGVGSVSEQALMA
                     EALEDPAVLDQAVRDLMDPPFPVVDVDWPLDR"
     gene            complement(1255..1836)
                     /locus_tag="AC16JC_04385"
     CDS             complement(1255..1836)
                     /locus_tag="AC16JC_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR5163969.1"
                     /translation="MIREKGPVLLAFGVLACGGSGPAPSAGSAPAEEPPVAVNAEPAV
                     QYPPDLYDRRVEGDVVLRLFVDSSGRLSPESTRVSESSGFAALDSAAVRGASRLRYAP
                     ARRHGLPVATAFLQTVEFRHPGGGVAPAPPAAPAATPQATPSRRDTVRHQPVAPPDSV
                     RARRDSSARKPDSTPPPRPDSARTQPDSNASAP"
     gene            complement(1833..>3436)
                     /gene="mutS"
                     /locus_tag="AC16JC_04390"
     CDS             complement(1833..>3436)
                     /gene="mutS"
                     /locus_tag="AC16JC_04390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HIR5163970.1"
                     /translation="RGRDRVRGSLEGVRDLERLASRAAMGRATPRELGSLRDSMLLLP
                     EVAAALESVVGRERAALLEQAGDDLDPLADLAAELSRALVDRPPAQTADGDAIRPGFD
                     TELDELRGMRDGGKQYIAALQARERERTGIGSLKVGYNRVFGYYIEVTRSNLAQVPAE
                     YERRQTLAGAERYVTPELKEYEAKVLGAEEHIAVREAELLDALRRRVGEGVARVQRTA
                     AVLARLDVWAGLADVAERERYTRPEVTDEFALVLEESRHPVVERMMPREAFIPNDVRL
                     DEAGRLILLTGPNMAGKSTLLRQVGLCVLLAQIGAFVPARRAVIGAVDRLFTRVGASD
                     NLVRGQSTFMVEMSETSAILHGATVRSLVLLDEIGRGTSTYDGVAIAWAVSECLHNEI
                     GCKTIFATHYHELTQLTESLAHARNFNVAVREAGDEIVFLHRMEAGGADRSYGIHVGR
                     LAGLPAPVVARAADVLRLLESEHHVAGRPVPPPPDAAQLALFGGENPPHPLLEELARL
                     DVDALSPLEALTRLHEWQRQLQENG"
BASE COUNT          487 a         1243 c         1158 g          548 t
ORIGIN      
        1 cggtcgagcg gccagtccac gtcgaccacc ggaaaggggg gatccatcag atcgcgcacc
       61 gcctggtcca gaaccgccgg atcctcgagc gcctcggcca tcagcgcctg ctcgctcacc
      121 gagccgacgc cgtcggctcc gtcgagcacc gggagctgcg agacgttgaa tgtgctcatg
      181 agattcagcg cctgtcggac ggttgcctgg gaccccacgg agacgagcgg cggggcccca
      241 tggtccttct gggcgacgag gtccgccacc gttcgccgct cggcttccag cagctggttc
      301 tcccgtagcc actcgtcgtt gtagaccttc gagaggtacc gctcgccgct gtcgttcagc
      361 accgtcacca cgcacgcctc tgggtcgtcc aggcgccgtg cgacgtccag cgcgctcacc
      421 agattgaccc cggtcgagcc gccgcacaac agcccttcct ctcgcgccag ccggcgcgtc
      481 atctgcatcg cgtcccggtc gctcacgacg atccattcgt cgatcaagtc gaagtgcagc
      541 gaggtaggga tcttgtcgcc gccgatcccc tcgactttgt agggagcccc ttcagccttg
      601 cggtgactcg ccgcgtattc cctgtaaatc gagccgaccg ggtcgccggc gatcaccctg
      661 atgttcgggt ttcgctcctt aaggaaacgc gcggtgccgc tcactgtacc gccggttccc
      721 ggagcgcaca cgaagtgcgt gatcctgccc tcggtctgag cccacaactc cggtccggtc
      781 gtgcggtagt gggcctcggg gttgaccgga ttgtagtgct ggtcggcgaa gatcgcattg
      841 gggagcgagg cggcgatcgc ccggcctttc atgatgtagc tgtccgggtg atccggtgcg
      901 accgccgtgg gcgtgatgat cacttcggct cccagcgccc gcaacagccg ggctttctcc
      961 tgcgacatct tgtccggcat ggtgaagatg cagcggtagc ccttgaccgc cgccgtgagc
     1021 gccaggccca ctccggtgtt gcccgaggtg gcctcgacga tgacgccgcc cggcttgagc
     1081 cgaccctcgc gctcggcttc ctcgaccatg gcgagcccga tccggtcctt caccgagccg
     1141 ccgggattga actgctccgc cttggcgtag accggcgtac gaatgcccgc catcacccgc
     1201 tgcaggcgga tgagcggcgt ccagccgatc gtctcaagga cattggcgta gggcttaagg
     1261 tgctgaggca ttcgagtcgg gctgcgttct cgcgctgtcg ggccggggcg gcggtgtgct
     1321 gtccggtttc cgcgcgctcg aatcgcgccg agcccggacg ctgtccggcg gggccacggg
     1381 ctgatgccgg acggtatcgc ggcgcgaagg cgtggcctgc ggcgtggcgg cgggggcggc
     1441 aggtggggcg ggcgccacgc ctccgcccgg atggcggaat tcgacggtct gcaggaaggc
     1501 ggtggcgacg gggagcccgt gccggcgggc gggagcgtag cgcagccggg acgccccgcg
     1561 caccgcggcc gagtcgagcg ccgcgaaacc gctcgactcc gacacccgcg tcgattccgg
     1621 actcaggcgg ccgctcgaat cgacgaacag ccgcagcacc acgtcaccct cgactcggcg
     1681 gtcgtacaga tcgggcggat actggaccgc gggctcggca ttgacggcca ccggtggctc
     1741 ttccgcgggc gcactcccgg cggacggtgc cggtcccgac cccccgcagg cgaggacgcc
     1801 gaacgcgagc agcaccgggc ccttctcccg gatcatccgt tctcctgcag ctgacgctgc
     1861 cattcgtgca gccgcgtgag tgcctcaagt ggggacagtg cgtcgacgtc cagccgcgcg
     1921 agctcttcga gcaaggggtg cgggggattc tcgccgccga acagggcaag ctgcgcggca
     1981 tcgggcggcg gcggcacggg gcgtcccgcc acgtggtgct cggattcgag cagccgcagc
     2041 acgtccgccg cccgcgcgac caccggcgcg ggcaggccgg cgagccgccc cacgtggatc
     2101 ccgtaggatc ggtccgctcc acccgcctcc atccggtgca ggaagacgat ctcgtccccc
     2161 gcctcgcgca ccgccacgtt gaagttgcgg gcgtgcgcca gactctcggt cagctgggtg
     2221 agctcgtggt agtgggtggc gaagatcgtc ttgcagccga tctcgttgtg caggcactcg
     2281 ctcaccgccc acgcgatcgc cactccgtcg tatgtggacg tgccgcgccc gatctcgtcg
     2341 agcagcacga ggctgcggac agtggcgccg tgcaggatcg cgctcgtctc actcatctcc
     2401 accatgaagg tggactgccc ccgaaccagg ttgtcggacg cgcccacgcg cgtgaacagc
     2461 cggtccaccg cgccgatcac cgcccgtcgc gcggggacga aggccccgat ctgtgccagc
     2521 agcacgcaca accccacctg gcgcagcagc gtggattttc cggccatgtt gggaccggtg
     2581 agcaggatca gccgtcccgc ctcgtcgagc cggacgtcgt tgggaatgaa cgcctcgcgc
     2641 ggcatcatgc gctccaccac cggatggcgg ctctcttcca gcacgagcgc gaactcgtcg
     2701 gtgacctccg gacgggtgta tcgttcgcgc tccgccacgt cggcgagacc ggcccatacg
     2761 tcgaggcgcg ccagcacggc ggcggtgcgc tgcacccggg ccaccccctc gcccacgcgg
     2821 cgccgcaggg cgtcgagcag ctccgcctca cgcacggcga tatgctcctc ggcacccagc
     2881 accttggctt cgtactcctt gagctcgggg gtgacgtatc gctcggcccc ggcgagcgtc
     2941 tgtcgccgct cgtactccgc cggcacctgg gcgaggttgg agcgcgtcac ctcgatgtaa
     3001 taaccgaaga cacggttgta gccgaccttg agggacccga ttccggtccg ctcgcgctcg
     3061 cgcgcctgca gcgccgcgat gtactgcttg ccgccgtcgc gcatcccgcg cagctcgtcg
     3121 agctccgtgt cgaagcccgg tcggattgcg tcaccatcgg cggtttgcgc cggcggacgg
     3181 tccaccagcg cccgggataa ttcggcggcc aggtccgcca gcggatccag gtcgtccccc
     3241 gcctgctcga gcagggcggc tcgttcgcgg ccgacgacgc tttccagtgc agcggcaact
     3301 tccgggagca gcagcatcga atcccgcagg ctgcccagct ctcgcggcgt cgcccggccc
     3361 atggcggcac gagatgccag tcgctcgagg tcgcgcaccc cttcgagcga tccgcgcaca
     3421 cgatcccgcc cgcgca
//
LOCUS       DBNGZW010000181         2749 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1783893, whole genome shotgun sequence.
ACCESSION   DBNGZW010000181 DBNGZW010000000
VERSION     DBNGZW010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2749)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2749)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2749
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1783893"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1487)
                     /gene="pckA"
                     /locus_tag="AC16JC_04395"
     CDS             complement(<1..1487)
                     /gene="pckA"
                     /locus_tag="AC16JC_04395"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012567974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HIR5163971.1"
                     /translation="MLLGFAPERAVYWNLGPAALYEHAVRRGEGVLAEAGPFCAVTSP
                     HTGRSPGDKFVVREPAAADQVWWGKVNQPLEPESFDRLYADVMRHLNGQELFARDLFA
                     GADPAYRIPVRIVSPNAWHTLFVYNMFLRPTDADLAAFKPAWHVLHAPEFQADPATHG
                     TKSGTFIVLHFARRLVLIGGTRYAGEMKKSVFTVLNYLLPAQGVLPMHCSANIGEAGD
                     VALFFGLSGTGKTTLSADPSRGLIGDDEHGWSESGVFNFEGGCYAKVIRLSAEGEPEI
                     FATTQRFGTVLENVVVDPVARTLDLDSAAITENTRASYPIHFIPNHVPGGLGGHPSHI
                     VFLTCDAFGVMPPIARLTPAQAMYHFLSGYTAKVAGTERGVTEPKETFSACFGAPFLP
                     RHPNAYATMLGEKIARHGVQCWLVNTGWTGGGFGTGQRIRLSHTRAIVRAALGGKLDR
                     VPTVREPAFGLAVPTEVPGVPADVLLPRRTWADPAAYDAQAMRLA"
     gene            complement(1549..>2749)
                     /locus_tag="AC16JC_04400"
     CDS             complement(1549..>2749)
                     /locus_tag="AC16JC_04400"
                     /inference="COORDINATES: protein motif:HMM:NF007233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HIR5163972.1"
                     /translation="EPVMRAYGLERLRFGPEYLIPKPFDPRVLLWVAPAVAWAAVGSG
                     VAGRVIDVEEYRAELDARLGRARGVMRGLSQRVQHDPQRIVFPEGEDARILRAARVLA
                     DDGAARPILLGDVDVMRRQADNAGVTLEEIELLDPRRAANAETMAQELWRRRQRRGIT
                     LREARARIADPMYYGLLMLRQGLADAFVAGADMHYAEAIRPALEVIGLEPGRRHTSGI
                     YMLVFQQQTLFFADCTVNIDPDAETLAEIASATARFVWRLGIEPRVALLSCSNFGSVK
                     HPSVEKVRRAVQLLHEREPELQVDGEMQADTAVVERILARTYPFARLRTAANVLIFPN
                     LDAANISYKLLDRLGGAEVIGPMLVGMAQPVHVLQRGAEVSDIVNLAVLAALDAFEHR
                     RHARPTH"
BASE COUNT          431 a          927 c          982 g          409 t
ORIGIN      
        1 tccgccagcc gcatcgcctg ggcatcgtac gcggcggggt cggcccaggt gcggcgcggc
       61 aagagcacgt ccgcggggac gccggggacc tcggtcggga cggcgaggcc gaaggcgggc
      121 tctcgcacgg tcgggacccg gtccagtttt ccgccgagcg cggcgcggac gatcgcccgc
      181 gtgtgggaga ggcggatgcg ctgtcccgtc ccgaatccgc cgccggtcca tcccgtgttc
      241 acgagccagc actgcacgcc gtggcgggcg atcttctcgc ccagcatggt ggcgtacgcg
      301 ttggggtggc ggggcaggaa cggcgcgccg aagcaggcgg agaaggtctc cttcggttcg
      361 gtgaccccgc gctcggtccc ggcgaccttc gcggtgtagc ccgacaggaa gtggtacatc
      421 gcctgcgccg gggtgagtct cgcgatgggc ggcatgacgc cgaacgcgtc gcaggtgaga
      481 aacacgatgt gcgacggatg gccgccgagc ccgccgggga cgtggttcgg gatgaaatgg
      541 atcgggtagg acgcgcgtgt gttctcggtg atcgccgcgg aatccaggtc cagcgtccgg
      601 gccaccgggt ccaccacgac gttctcgagg accgtgccga agcgctgcgt cgtggcgaag
      661 atctccggtt ccccctcggc ggagaggcgg atgactttcg cgtagcaccc gccctcgaag
      721 ttgaagacgc cggactcgct ccacccgtgc tcgtcgtcgc cgatcagccc gcgcgaggga
      781 tcggcggaga gcgtcgtctt gccggtcccc gagagcccga agaacagcgc gacgtcgccc
      841 gcttcaccga tgttcgccga gcaatgcatc ggcagcacac cctgggccgg gagcagataa
      901 ttgagcaccg tgaagacgct cttcttcatt tccccggcgt accgggttcc gccgatcaac
      961 accaggcggc gagcgaaatg gaggacgatg aacgtgccgc tcttcgtgcc gtgggtggcg
     1021 gggtcggcct ggaactcggg agcgtgcagc acgtgccacg ccggcttgaa ggcggcgagg
     1081 tccgcgtcgg tgggccgcag gaacatgttg tacacgaaca gcgtgtgcca cgcgttgggc
     1141 gagacgatcc gcaccggaat acggtaggcg gggtcggccc cggcgaacag atcgcgtgcg
     1201 aacagctcct ggccgttcag gtggcgcatc acgtcggcgt acaggcggtc gaaggattcg
     1261 ggctcgagcg gctggttcac cttgccccac cacacctgat cggcggcggc gggctcccgc
     1321 acgacgaatt tgtcgcccgg gctgcggccg gtgtgggggc tggtgacggc gcagaacggg
     1381 cccgcctcgg ccagcactcc ttcaccgcgg cgtacggcgt gctcgtacag cgcggcgggg
     1441 ccgagattcc agtacaccgc ccgttcgggg gcgaagccca gaagcatcgc gccagaaggg
     1501 cgggcgccgc cggtgggcga tggggcaatg atctgcatga gagatccttt agtgcgtagg
     1561 ccgcgcgtgg cggcgatgtt cgaaggcgtc cagagcggcg agcaccgcca gattgacgat
     1621 atcgctcact tcggctcccc tttgcaagac gtgtaccggc tgggccatcc cgaccagcat
     1681 cggtccgatg acctcggcgc cgcccaaccg gtcgagcaac ttgtaggata tgttcgcagc
     1741 gtcgaggttg gggaatatca acacgttcgc ggcggtgcgc agccgggcaa aggggtaggt
     1801 cctggcgaga atgcgctcca ccacggccgt gtcggcctgc atctcgccat ccacctggag
     1861 ctcgggctcg cgctcgtgca gcaactggac ggcgcgccgc accttctcca ccgaggggtg
     1921 tttcacggac ccgaaattcg agcaggacag gagcgcgacg cgcggctcga tgccgagacg
     1981 ccagacgaac cgcgccgtgg cggacgcgat ttcggccagc gtctcggcgt cggggtcgat
     2041 attcacggta cagtcggcga aaaacagcgt ttgttgctgg aacaccagca tgtagatgcc
     2101 gctggtgtga cgccggcccg gttcgagccc gatcacctcg agcgccggcc ggatcgcttc
     2161 ggcgtaatgc atgtccgctc ccgcgacgaa ggcgtcggcc agcccctgcc gcagcatcag
     2221 caatccgtag tacatcggat ccgcgatgcg agcccgggcc tcgcgcaggg tgatgccgcg
     2281 gcgctgccgg cgccgccaca actcctgcgc catcgtctcg gcgttcgccg cgcgccgcgg
     2341 atccagcagc tcgatctcct cgagcgtgac acccgcattg tcggcctggc gccgcatcac
     2401 gtcgacgtct ccgagcagga tcgggcgggc ggcgccgtcg tcggccagca cccgcgccgc
     2461 gcgcagaatg cgcgcgtcct cgccttccgg aaacacgatg cgctgcgggt cgtgctgcac
     2521 gcgctgcgac aacccgcgca tcaccccacg cgcccgtccg agccgggcgt ccagctcggc
     2581 gcggtactcc tcgacgtcga tcacccggcc ggcgacgccg gagccgacgg ccgcccaggc
     2641 cacggccggg gcgacccaca ggaggacgcg cgggtcgaac ggcttgggaa tcaggtattc
     2701 ggggccgaac cgcagccgtt ccagtccgta ggcgcgcatc accggctcg
//
LOCUS       DBNGZW010000182         9460 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1375301, whole genome shotgun sequence.
ACCESSION   DBNGZW010000182 DBNGZW010000000
VERSION     DBNGZW010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 9460)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9460)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9460
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1375301"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..223)
                     /locus_tag="AC16JC_04405"
                     /pseudo
     CDS             complement(89..223)
                     /locus_tag="AC16JC_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014406299.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
     gene            complement(223..429)
                     /locus_tag="AC16JC_04410"
     CDS             complement(223..429)
                     /locus_tag="AC16JC_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012267.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HIR5163973.1"
                     /translation="MSPIAARAAEVAVVDLDRCDRCGLCLPLCPPEAIHLQLSDLVID
                     RAGCTGCHKCIAPCPVGALAMTPA"
     gene            556..1599
                     /locus_tag="AC16JC_04415"
     CDS             556..1599
                     /locus_tag="AC16JC_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR5163974.1"
                     /translation="MPIRWPKWKLGAFNPANEIAVDLGTANTLVYVRGEGIVLNEPSV
                     VAIEKATGKVKGIGLEAKRMLGRTPDGILAVRPLKDGVIADFDVTEKMLRYFLKTIID
                     RHVFRVRPKVIVCVPSGITEVERRAVRDSAQSAGAKEVYMVAEPMAAAIGVGLPVETP
                     TGNMVIDIGGGTTEIAVIALSGIVSDTSIRTAGDELDQAIVQFMRKNYNLLIGEPTAE
                     QIKIQIGSAAPVGEEREMEVKGRDLVSGIPKIVRVHSSAVREAVQEPIQQIVDAVRRA
                     LEITPPELASDIVDRGIVMTGGGALIRGLDQLLQQETGLPIHLDEDPMTCVVRGTGRI
                     LDDPEKYRNVLTT"
     gene            1605..2465
                     /gene="mreC"
                     /locus_tag="AC16JC_04420"
     CDS             1605..2465
                     /gene="mreC"
                     /locus_tag="AC16JC_04420"
                     /inference="COORDINATES: protein motif:HMM:NF016011.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR5163975.1"
                     /translation="MALDADRYATRGDTLAFLSSLALSLVAMSLPPTVRDSVASGLRR
                     SVLWPLLQLQEQSEALRTSHARFVEVSAERDSAVLAAAQVQQLRSENSQLRDLLGLRA
                     RLGTTFVPAEVLHQAALTDRFTLVISAGTRQGVRPLAAVVTSEGLLGFVSKAEPVTSV
                     VATWAHPDFRASGMAADGSVYGILSPRGSSGSGSWVLQLDGVPYRQRVPAGGVILTSG
                     LGGVLPRGIPVGTVVGEITGENDWERTYLVRPAVPPGSATHVMVLVQPPQGDLRSSFA
                     PDSAAGRRQP"
     gene            2462..2956
                     /gene="mreD"
                     /locus_tag="AC16JC_04425"
     CDS             2462..2956
                     /gene="mreD"
                     /locus_tag="AC16JC_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR5163976.1"
                     /translation="MRRDSVRFWGILALLVLLQFSIRSRLGNERVAPDFLLLALLIYT
                     IRARPGASAAVGFLVGLISDALTPASFGTGALAHTVVGYLSSYAKAVFFAENLLVNGG
                     LIFAGTWLRNLIVAVASGKFAGPQLEWELLVWSPLEALTTAAAGVLVLTLFRQWLAIR
                     VEES"
     gene            2953..4821
                     /gene="mrdA"
                     /locus_tag="AC16JC_04430"
     CDS             2953..4821
                     /gene="mrdA"
                     /locus_tag="AC16JC_04430"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR5163977.1"
                     /translation="MKRLTPHQLDAKARGARLLVWAAGAVFAVAYFRVQILDHHKYDV
                     QSEYNRLRLIPMPAPRGLITDRHGTVIAENVPGYTVTLLPAPEDSLRRRLQRIAPIVH
                     LDAAGIERVMQRFRRSRYQPAVVLTDAPFEVVSALEERRLTLPGVVIQPEPKRHYPDS
                     AMVAHLVGIVGEVTDQDLVTRRDSSLRLGSLIGKDGLERAYDDTLRGQDGERFVEVSA
                     RGYVVREAGAAQTLQPEPGATLRTTIDLDLQRYIARVFPPGMRGAIVALLPSTGEILA
                     LYSAPTYDPNAFVGGVSPEYWRALNQSDAHPLLDRAIQARYPPGSTWKLAIAAMALRR
                     GVATMGTRMSIPCRGGMQYGNRFFRCWSPGGHGSLTLEQAIAQSCDVYFYQLGLKLGL
                     TSLLEDANALGLRSRTGVDLPGEMAPQFPSGTEYYDRLYGPRRWTSAVSLNLAIGQGE
                     NSQTLLDMVRLYQMLASDGKSRTPYLVRPQTTGPTYDLGLSPEQLAGLRQAMINVVER
                     GTGQGARLAELKIAGKTGTAQSTQGINHGWFIGFAPADSPRVVVGVIGEYSGHGAALA
                     PLVARVIAHYLGADTTTARYLFTLPRDTAPRDTALLGAPPDSLPFIPDTTRAPSIP"
     gene            4802..6055
                     /gene="rodA"
                     /locus_tag="AC16JC_04435"
     CDS             4802..6055
                     /gene="rodA"
                     /locus_tag="AC16JC_04435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR5163978.1"
                     /translation="MLRRFRELDRPLLGVVAALAVYGLLILYSAGQTDVPTVVATIWL
                     RQLVWLVLGVTCGVLVFRISPRLLEWAAPYVYGTAIFVLLVTLVFGSGAGTAAGSRSW
                     ITLGGHRLGQPAELAKIAVILMLARWLAARREPPASLRDLVYPGAITLVPFGLVVLQP
                     DLGSAIVFIAILFAMLYWAGAKPGMLLLLVSPVIGLVLAFSTAVWGTWIVLLFALVLW
                     IRPYLWEGLTVMLANIVMGVVALPFWRHLAPYQQSRFLAFLNPEADPRATGWHIIQSK
                     IAIGSGGWFGKGFTHGSQKRLAFLPAQHTDFIFPIVGEELGFAGVLLALGLFAALLVI
                     LLRIARRATDPYSSFCVFGIAGMLFTHIVENVGMAVNLLPVTGIPLPFFSYGGSFLLT
                     CALAVGIVLRVAWESRQSGYADLSQ"
     gene            6131..6988
                     /gene="accD"
                     /locus_tag="AC16JC_04440"
     CDS             6131..6988
                     /gene="accD"
                     /locus_tag="AC16JC_04440"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HIR5163979.1"
                     /translation="MAWFKKERKPRTSERVRLEIPADAWEKCDECGHIDIKERFARAL
                     SVCPNCGAHRRIAAQEYIDLLVDEGSWHELDQSLRSADPLKFENYRERMQAAIKKAGP
                     FEAIRTGYGRLDGMAINLGVMDFAFMGGSMGSVVGEKVARLARRSIDRRVPLIIVSAS
                     GGARMQEGVLSLMQMAKASVVVAELGERGVPFVSILTNPTTGGVSASYAMQGDVILAE
                     PGAVVGFAGPRVIKQTIGQDLPEGFQTAEFLLEHGMVDAVVPRSELRETTAQLLRHMA
                     GRAPAEAGD"
     gene            7069..8205
                     /locus_tag="AC16JC_04445"
     CDS             7069..8205
                     /locus_tag="AC16JC_04445"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HIR5163980.1"
                     /translation="MNLLEAIGHPERQFPSIHVGGTNGKGSTCAFLTAALRARGFRVG
                     LYTSPHLVSVRERVVVNGEPMSEAAFAEWTALLEPHIERTEASFFEAMTAIGFADLAS
                     RDVDIAVIEVGLGGRLDSTNVLTPLASVVTKIALEHTDYLGDDLASIAREKAGIAKRG
                     VPFVTGEQDPVVRQVLIEEAQRLGAHPIVRATAGQTPEGLELGLRGPHQWTNAWVALG
                     TLNALPPPFGPVGPWMPASFAEAYVPGRFDVRGRWIFDVAHNPDGMAVLVAALREFDP
                     PRPLHALVGIRNDKDWPAMLAAIRPEVDRLLLTVAPSVPGHQRWAERDFSDGRLSEAS
                     VRFEPDFDRALAGIQEGARTVLVTGSFHTVGDAMARLPGFAPIG"
     gene            8215..>9460
                     /gene="hisS"
                     /locus_tag="AC16JC_04450"
     CDS             8215..>9460
                     /gene="hisS"
                     /locus_tag="AC16JC_04450"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008104876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HIR5163981.1"
                     /translation="MQGKALPGFRDFYPDDLALRTYIFSTWRSVATRYGFEEYDGPPL
                     EPLELYTQKSGEEIAAQLYAFRDKGDREVALRPEMTPTLARMVGARAQALRKPIRWFS
                     IPQLFRYERQQKGRLREHFQLNMDLIGEPGPLADAELIAAAIDVLRAFGLDQGDVQAR
                     VSDRRVLRTLLLGGGVSEAQLPAAYAAIDKSERDPRDALAARLQGAGLAPAAVEAVFA
                     VAQLRGVEAVDAALARVRDGEAAGESLRECARALRAMGLGDFVQVDLTIVRGLAYYTG
                     VVFELFDAGRSLRAICGGGRYDGLLEALGGADLPALGFGMGDVVLGELLKSRGAVPAG
                     SSRLDLFLVAVGGEDLATVLRLAHELRGRGLAVEYVLRLQAIRKQLELAASRGAPRAV
                     IIGPDERAAGQVVLRDLQRGTEE"
BASE COUNT         1352 a         3132 c         3456 g         1520 t
ORIGIN      
        1 ggaagatcac ccgcgtgatc ttgcgggccg cccacggcgc gcctgcgggg tccgcgaact
       61 cccgcagccc ggcgtcaccc actccctacg cgcagcgcac cggagcgccc acggcccgat
      121 cgcgctcgag caggacgacc tcgaggccgg cacccgccgc ctcgagcgcg gcgatcgagc
      181 cggcaggccc ggcccccacc accaggatgt cgatctcgcg catcaggccg gggtcatcgc
      241 cagcgctccc accgggcagg gcgcgatgca tttgtgacat ccggtacagc cggcgcggtc
      301 aatgaccagg tcgctgagct gcaggtggat agcttccgga ggacaaaggg gcaggcacag
      361 gccgcagcgg tcgcagcgat ccagatcgac gacggccacc tcggccgccc gtgcggcgat
      421 gggggacatg ggggtaggct agccgtccgc cggaggggcg tcaagcatca gccttgcttg
      481 gagttacgct tttcgttagg ttcatgcgcg cgcgacacac ccgcccttac acataaccgg
      541 aaccgatcgc cgaccgtgcc gattcgctgg ccgaaatgga agctcggggc gttcaacccc
      601 gcgaacgaga tcgccgtgga tctgggcacg gcgaacacgc tcgtgtacgt gcgtggggaa
      661 gggatcgtgc tgaacgagcc gtccgtcgtg gcgatcgaga aggcgacggg caaggtgaag
      721 ggcatcgggc tggaggcaaa gcggatgctg gggcgcacgc ccgacggcat tctcgccgtg
      781 cgcccgctga aggacggcgt gatcgccgac ttcgacgtca ccgagaagat gctgcggtac
      841 ttcctgaaga cgatcatcga ccgccacgtg ttccgcgtgc ggccgaaggt gatcgtctgc
      901 gtgccgagcg ggatcaccga ggtggagcgc cgcgcggtgc gcgactcggc gcagtcggcg
      961 ggcgcgaaag aggtgtacat ggtcgccgag ccgatggcgg cggctatcgg ggtggggctg
     1021 ccggtcgaga cgcccaccgg caacatggtg atcgacatcg gaggcgggac caccgagatc
     1081 gcggtgatcg cgctgtcggg gatcgtgtcg gatacgtcga tccggaccgc gggggacgag
     1141 ctggatcagg cgatcgtcca gttcatgcgg aagaactaca atctcctgat cggcgagccg
     1201 acggccgagc agatcaagat ccaaatcggc tcggcggccc ccgtgggcga ggagcgtgag
     1261 atggaggtca agggacggga cctcgtctcg ggcatcccga agatcgtgcg ggtccactcg
     1321 agcgcggtcc gcgaggcggt gcaggagccg atccagcaga tcgtggatgc cgtgcgtcgc
     1381 gccctcgaga tcacgccgcc ggagctcgcc agcgacatcg tggatcgcgg catcgtgatg
     1441 accggaggcg gggcgctgat ccgcgggctc gaccagctgc tgcagcagga aaccggattg
     1501 ccgattcacc tcgacgagga ccccatgacg tgcgtggtac gggggaccgg ccggatcctg
     1561 gacgatcccg agaagtatcg caacgtcctg acgacctagc tgccatggcg ctcgacgcgg
     1621 accggtacgc cacccgcggt gacacgctcg cgttcttgag cagcctcgcc ctctcgctcg
     1681 tcgccatgag cctgcccccg accgtgcggg attccgtcgc gagcggattg cggcgcagcg
     1741 tgctgtggcc gctcctccag ctgcaggagc agtccgaggc gctccggaca tcccacgctc
     1801 gattcgtcga ggtgagcgcc gagcgcgact cggcggtgct cgccgcggcg caggttcagc
     1861 agctgcgctc cgagaactcc cagctgcgcg atctcctggg gctgcgcgcc cgcctgggca
     1921 ccacgttcgt tccggcggag gtgctccatc aggccgcgct caccgaccgc ttcaccctgg
     1981 tcatctcggc cggcacacgg cagggggtgc gtccactcgc ggcggtggtg acgtccgagg
     2041 ggttgctcgg cttcgtgagc aaggccgagc ccgtgacgag cgtggtggcg acctgggcgc
     2101 acccggactt ccgggcgagc ggcatggcgg cggacggcag cgtgtacggc atcctctcgc
     2161 cccgcggaag ctcgggttcg ggaagctggg tgctccagct ggacggcgtg ccgtatcgcc
     2221 agcgggtgcc ggccgggggc gtgatcctca cgtccggatt gggcggcgtg ttgccgcggg
     2281 gcatcccggt cggcacggtc gtcggtgaga tcacgggcga aaacgactgg gagcggacct
     2341 acctggtccg gcccgccgtg ccgccgggct cggcaacgca cgtgatggtg ctggtccagc
     2401 ccccccaggg ggacctgcgc tcgtccttcg cgccggattc ggcggccgga cggcgccagc
     2461 catgaggcgc gacagcgtgc ggttctgggg aatcctggcg ctgctcgtac tgctccagtt
     2521 ctcgattcgc agccggctcg gcaacgaacg ggtggcgccc gatttcctgc tgctggccct
     2581 gctcatctac acgatccggg cccgccccgg cgcgagcgcg gcggtggggt tcctggtcgg
     2641 cctgatctcc gatgcgctca cgccggcgag cttcggcacc ggcgccctgg cccacacggt
     2701 cgtcggctac ctctcgtcct acgccaaggc cgtcttcttc gcggagaacc tgctcgtcaa
     2761 cggcgggctg atcttcgcgg ggacgtggct gcgcaatctg atcgtcgcgg tcgcctcggg
     2821 caagttcgcg ggcccgcagc tggagtggga gctgctggtg tggtcgccgc tggaagcgct
     2881 cacgacggcc gccgcgggag tgctcgtcct gacgttgttc cgccagtggc tggcgattcg
     2941 tgtggaggag tcatgaagcg cctgacgccc caccagctcg acgcgaaggc gcgcggcgcg
     3001 cggctcctgg tctgggcggc cggcgccgtg ttcgcggtcg cctacttccg cgtccagatc
     3061 ctggatcatc acaagtacga tgtgcaatcg gaatacaacc gcctgcggct gatcccaatg
     3121 cccgcgccgc gcggactgat caccgaccgc cacggcaccg tcatcgccga gaacgtgccc
     3181 gggtacacgg tcaccttgtt gcccgctccc gaagactcgc tgcgccgcag gctgcagcgc
     3241 atcgcgccga tcgttcatct cgacgccgcg gggatcgagc gggtgatgca gcggtttcgc
     3301 cggtcgcggt atcagcccgc cgtggtcctg accgatgcgc cgttcgaggt ggtttccgcg
     3361 ctcgaggagc gtcgcctcac cctccccggg gtggtgattc aacccgagcc gaagcgtcat
     3421 tacccggaca gcgcgatggt ggcccatctg gtgggcatcg tcggcgaggt gacggaccag
     3481 gacctcgtga cgcggcggga cagctcgctg cgcctgggct ccctgatcgg caaggacggt
     3541 ctcgagcggg cgtacgacga tacgctgcgc gggcaggatg gtgagcgatt cgtcgaggtg
     3601 agcgcccggg gatacgtcgt gcgggaagcc ggggcggcgc agacgctgca gcccgaaccc
     3661 ggggccacac tgcgcaccac gatcgacctc gacctgcagc gctacatcgc ccgggtgttc
     3721 ccgcccggga tgcgcggcgc gatcgtcgct ctgctgccgt ccaccgggga aatcctggcg
     3781 ctgtacagcg cgccgacgta tgaccccaac gcgttcgtgg gcggcgtgtc gcccgagtac
     3841 tggcgcgcgc tcaaccagag cgatgcgcat ccgctgctgg atcgcgcgat ccaggcccgg
     3901 tatccgccgg ggtccacctg gaagctggcc atcgccgcca tggccctgag gcgtggcgtc
     3961 gcgacgatgg gtacccggat gtcgatcccc tgccggggcg gcatgcagta cggcaatcgg
     4021 ttcttccgat gctggagccc cggcggccac ggcagcctga cgctggagca ggcgatcgcg
     4081 cagagctgcg atgtctactt ctaccagctg ggcctgaaac tgggcctcac gtcgctgctg
     4141 gaggacgcca acgcgttggg gttgcgctcg cgcaccggcg tggacttgcc gggcgagatg
     4201 gccccccagt tcccgagcgg caccgagtat tacgaccggt tgtacggccc gcgacgctgg
     4261 acctccgccg tctcgctcaa cctggcgatc ggccaggggg agaacagcca gacgctgctc
     4321 gacatggtgc ggctctacca gatgctggcg agcgacggga agtcccgcac gccgtatctc
     4381 gtgcgaccgc agaccaccgg gccgacctac gatctcggct tgagccccga acagctcgcc
     4441 ggcctgcgcc aggcgatgat caacgtggtg gagcgcggga ccgggcaggg cgcccgcctg
     4501 gccgagctca agatcgccgg caagaccggc accgcgcaga gcacgcaggg gatcaatcac
     4561 ggttggttca tcgggttcgc tccggcagac agcccgcggg tggtggtggg ggtcatcggg
     4621 gaatactccg gacacggagc cgcgttggcg cccctcgtgg cccgcgtgat cgcccattac
     4681 ctgggcgccg acactacgac ggcgcggtat ctattcacgc tcccgcgcga caccgcgccg
     4741 cgcgacaccg cgctgctcgg tgcccctccc gattcgctgc ccttcatccc cgacaccacg
     4801 cgtgctccgt cgattccgtg agctcgaccg cccgctgctg ggcgtggtgg cggcgctggc
     4861 cgtgtacggg ctgctcatcc tgtattcggc ggggcagacc gacgtcccga ccgtggtggc
     4921 gacgatctgg ctgcgccagc tcgtgtggct ggtgctgggg gtcacctgcg gggtcctggt
     4981 gttccggatc tccccacggc tgctcgaatg ggcggcgccg tacgtgtacg gcacggccat
     5041 cttcgtactc ctggtcacgc tggtgttcgg gtcgggcgcg gggaccgcgg cagggagccg
     5101 gtcgtggatc accctcgggg ggcatcggtt ggggcaaccg gccgagctcg ccaagatcgc
     5161 cgtcatcctg atgctggccc gctggctggc ggcgcggcgc gagccaccgg cgagcctgcg
     5221 ggatctggtg tatcccgggg cgatcacgct ggtgccgttc gggctcgtgg tgttgcagcc
     5281 ggatctcggc agcgcgatcg tgttcatcgc gattctcttc gccatgctgt actgggccgg
     5341 tgccaagccg gggatgctgt tgctcctcgt gtcgcccgtc atcggcctgg tgctggcctt
     5401 ttccaccgcg gtctggggga cctggatcgt cctgctgttc gccctcgtgc tgtggatccg
     5461 tccctatttg tgggaagggc tgacggtgat gctggcgaac atcgtcatgg gcgtggtggc
     5521 cctgccgttc tggcgtcacc tggcgccgta ccagcagagt cggttcctgg cgttcctcaa
     5581 ccccgaggcg gatccacgcg cgaccgggtg gcacatcatc cagtcgaaga tcgccatcgg
     5641 ctcggggggg tggttcggca aggggttcac gcacggctcg cagaagcgac tggcgttcct
     5701 tcccgcccag cacaccgact tcatcttccc catcgtcggg gaagagctcg gcttcgccgg
     5761 cgtgctgctg gcactgggtc tgttcgcggc gctgctggtc atcctgttgc gcatcgcgcg
     5821 gcgtgccacg gacccgtact cgagcttctg cgtcttcggg atcgccggga tgctgttcac
     5881 ccacattgtg gaaaacgtcg gcatggcggt gaacctgctg ccggtgacgg gcatcccgct
     5941 gccgttcttc tcgtacggcg ggtcgttcct gctcacctgc gcgctggcag tagggatcgt
     6001 cctccgggtg gcatgggagt cccgtcaatc cggctatgcc gacctctcgc aatgagttac
     6061 gactagaggc ctagcgcggg gcggaacggg cggcgagatt gtccgcttcc gcacccccac
     6121 gaatcccggc atggcatggt tcaaaaagga gcgtaaaccc cgcacctccg agcgcgtcag
     6181 gctggagatc ccggccgacg cgtgggagaa atgcgacgag tgcgggcata tcgacatcaa
     6241 ggagcgcttc gcgcgcgccc tgagcgtctg cccgaactgc ggggcccatc gccggatcgc
     6301 agcgcaggag tacatcgatc tgctggtgga cgagggctcc tggcacgagc tcgatcagag
     6361 tctccggtcc gccgacccgt tgaaattcga gaactatcgc gagcggatgc aggccgccat
     6421 caagaaggcc ggtccgttcg aagcgatccg gacgggctac gggcggctgg acggcatggc
     6481 catcaacctg ggcgtgatgg atttcgcctt catgggcggc tccatgggat cggtcgtggg
     6541 tgagaaagtc gcccggctgg cccgccggtc catcgaccgg cgggtgccgc tgatcatcgt
     6601 gtcggcctcc ggcggcgcgc ggatgcagga gggggtcctg tcgctgatgc agatggccaa
     6661 ggcgtccgtg gtggtggccg agctggggga gcgcggcgtc cccttcgtgt cgatcctgac
     6721 gaacccgacc acgggcggcg tgtcggcgtc gtacgcgatg cagggggacg tgatcctcgc
     6781 cgagccgggg gcggtggtgg gattcgcggg tccgcgcgtc atcaagcaga ccatcgggca
     6841 ggacctgccc gaggggttcc agaccgcgga gttcctgctg gagcacggca tggtcgatgc
     6901 cgtcgtgccg cggagcgagc tgcgggagac cacggcgcag ctgctgcgcc acatggccgg
     6961 ccgggcgccg gcggaggcgg gcgactgagt cgctggtcct tccaggacgc gtgccgctat
     7021 ctgttccctc gacaagccgg acgcatcaag tggagcctcg agccgacgct gaacctgctc
     7081 gaggccatcg gccatccgga gcggcagttc ccctccattc acgtcggcgg cacgaacggg
     7141 aagggttcca cgtgcgcgtt cctgaccgcg gcgctgcggg cgcggggatt ccgcgtgggg
     7201 ctctacacgt cgccccacct ggtctcggta cgagagcgcg tggtcgtcaa tggcgagccg
     7261 atgagcgagg cggccttcgc ggagtggacc gcgctgctcg aaccgcacat cgagcgcacc
     7321 gaggcgagtt tcttcgaggc gatgaccgcg atcggattcg ccgacctggc ctcgcgcgac
     7381 gtggacatcg cggtcatcga ggtggggttg ggaggccggc tcgacagcac caatgtgctc
     7441 accccgctgg cgagcgtggt gaccaagatc gcgctggagc ataccgacta cctcggtgac
     7501 gatctcgcga gcatcgcgcg cgagaaggcc ggcatcgcca agcgcggggt cccgttcgtc
     7561 accggcgagc aggaccccgt ggtacgtcag gtgctgatcg aggaagcgca gcggctcggc
     7621 gcgcatccga tcgtgcgggc gaccgcgggg cagacgccgg agggcctcga gctcgggctg
     7681 cggggcccgc atcaatggac caacgcctgg gtggcgctcg gcaccctcaa cgccctgcca
     7741 ccgcccttcg gcccggtcgg cccctggatg cccgccagtt tcgcggaggc atacgtcccg
     7801 ggtcgcttcg acgtgcgcgg tcgctggatc ttcgacgtgg cccacaaccc cgacgggatg
     7861 gcggtgctgg tcgcggcgct gcgggaattc gatcccccgc ggccgctgca tgccctggtg
     7921 gggatccgca acgacaagga ctggccggcc atgctcgcgg cgatccgtcc cgaagtggac
     7981 cggctgttgc tcacggtggc cccctcggtg ccgggtcatc agcggtgggc cgagcgggac
     8041 ttcagcgacg ggcgcctgtc cgaagcgtcg gtgcggtttg agcccgactt cgaccgcgct
     8101 ctcgccggca tccaggaggg cgcgcggacg gtgctggtga ccggatcgtt ccacacggtg
     8161 ggcgatgcga tggcgcgctt gcccgggttc gcgccgatcg gctaaattgt tgtgatgcaa
     8221 gggaaagcgc ttcccggctt tcgcgatttc taccccgatg atctggccct acgcacctac
     8281 atcttctcga cctggcggag cgtcgcgacc cgctacgggt tcgaggaata cgacggtccg
     8341 ccgctggaac cgttggagct ctacacgcag aagagcgggg aggagatcgc ggcgcagttg
     8401 tacgcgttcc gggacaaggg cgaccgggag gtggcactgc ggccggagat gacgccgacg
     8461 ctggcccgga tggtcggagc ccgcgcccag gcgttgcgca aaccgatccg ctggttctcg
     8521 atcccgcagc tgttccgcta cgagcggcag caaaaggggc ggctgcgcga gcatttccag
     8581 ctgaacatgg acctgatcgg cgagcccggg ccgctcgccg acgccgagct gatcgccgcc
     8641 gcgatcgacg tgctgcgggc cttcggactg gaccagggcg acgtgcaggc ccgggtgtcg
     8701 gaccgtcggg tgctgcggac gctgctgctg ggcgggggcg tgagcgaagc gcagctcccg
     8761 gccgcctacg ccgcgatcga caagagcgag cgcgacccgc gcgacgcact cgccgcccgg
     8821 ttgcagggtg cggggctcgc gccggcggcg gtcgaggcgg tcttcgcggt ggcccagctg
     8881 cggggggtgg aggcggtgga cgcggcgctg gcgcgcgtgc gggatggaga ggcggcgggc
     8941 gaatcgctgc gggagtgcgc gcgggccctg cgggcgatgg ggctgggcga cttcgtgcag
     9001 gtcgacctga cgatcgtgcg cggcctggcc tactacaccg gcgtcgtgtt cgagctgttc
     9061 gacgccggac gcagcctgcg ggcgatctgc ggcggtggcc gctacgacgg cctgctcgag
     9121 gcgctgggcg gagccgatct gccggcgctg ggattcggca tgggggacgt cgtgctgggc
     9181 gagctgctga agtcgcgtgg cgcggtcccc gccgggtcgt cccggctgga tctctttctc
     9241 gtcgccgtgg gcggcgagga tctggcgacg gtgctgcgtc tggcgcatga gctgcggggg
     9301 cgcggcctcg cggtcgagta cgtactgcgg ctgcaggcga tccgcaagca gctcgagctg
     9361 gcggcgtcgc gcggcgcgcc gcgagcggtg atcatcggac ccgacgagcg cgcggccggc
     9421 caggtcgtgc tgcgcgatct gcaacgcgga accgaagaac
//
LOCUS       DBNGZW010000183         3334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_289033, whole genome shotgun sequence.
ACCESSION   DBNGZW010000183 DBNGZW010000000
VERSION     DBNGZW010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3334
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_289033"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="AC16JC_04455"
     CDS             complement(<1..563)
                     /locus_tag="AC16JC_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5163982.1"
                     /translation="MSATRVPWTAATLFVVTVSTVFHRLPPSSTDLQSREILLATTTS
                     TRDAGLLDSLLPVFERETGYQVKVVAVGSGQALALGRRGDADVVLSHAPEAERALADS
                     GYLLRRRLVMHNDFLIVGPAADPAGLRGLTDAVAALRRLAAARALFVSRGDRSGTNLL
                     ELKLWRLAAIEPPAAGGEGGWYLDAGQG"
     gene            596..1066
                     /locus_tag="AC16JC_04460"
     CDS             596..1066
                     /locus_tag="AC16JC_04460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine phosphatase family protein"
                     /protein_id="HIR5163983.1"
                     /translation="MSDLLDAISGVPNASEPIPGLVTGGQPLSRHLTALKQAGCELVL
                     DSRDPMEPRPIAEPEDAAAAGLEYVCVPMGHTRGEDATLSKIREVLRANVGKRKMFYH
                     CASGNRVGASVIPYLMLDQGFGEEDAVNAALRMGMRNPELLEWALDYVKRNASK"
     gene            complement(1208..1600)
                     /locus_tag="AC16JC_04465"
     CDS             complement(1208..1600)
                     /locus_tag="AC16JC_04465"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5163984.1"
                     /translation="MSAIPAYRHLIDAFGEGWEQADVDRICSVFTPDAVFVETPFSQK
                     DIGQAAIRAYWQDLPLTQADVSFRSGEIFEVGPWFATEFRCTFRRRSSGEWVDARGAI
                     FCETKDGKISEMRMYWHRYAGGREEPKP"
     gene            1708..2088
                     /locus_tag="AC16JC_04470"
     CDS             1708..2088
                     /locus_tag="AC16JC_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5163985.1"
                     /translation="MLKRILASAAVLGLLALPLSAQATPQANAGNKVTLTGQVIDVNC
                     YTTQHMQGAAHKACATACANKGVALAILASDGTIYMPVSSKPGDPQNPRLMQFVEGKV
                     KVTGTHRSVSGLHTIEIETVEAAS"
     gene            2100..>3334
                     /locus_tag="AC16JC_04475"
     CDS             2100..>3334
                     /locus_tag="AC16JC_04475"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HIR5163986.1"
                     /translation="MGRALGAVLCLWAASACRSTAPSGLTLLFYNRSPAAVLGGRSWA
                     PDAGSSRVLAFDDRLHPVKSLGGAALAMPMAVSTLGNRLLVTEETGEGVVLDTAGEGA
                     LVREWPSPFPIALYAAAGSHIVAARSPYRVPSLTPEAPDAPLVRVLDTLGRPLEGLAA
                     IHVPATPFLTQITNAGAIAVDSGGAVYYAPLVRDEIVKFGASGERRWTTTRNLRPSAS
                     DPVYLPAQGGGHDLLIEQATVNVALALGPDGRLYALGAEDSAATQLRVDVLDTATGAI
                     LATRRLGANETAVAVDGGGRIATFDAAVLARGVPAARQPFAPGFALPDTAGDTVTLAR
                     FAGKVTLVDFWASWCDPCREEFPHMVALYDRLDRADFEIVAISDDVDRGSMLAFIREF
                     HPPFPVLVGGGRMKQQYHYR"
BASE COUNT          546 a         1140 c         1122 g          526 t
ORIGIN      
        1 ccctgcccgg cgtcgagata ccagccgcct tcccctcccg cagcgggagg ctcgatcgcg
       61 gcgagccgcc acagtttcag ctcgagcagg ttcgtgccgg agcggtctcc acgggatacg
      121 aagagcgccc gggccgccgc gaggcgccgt agcgcggcca ccgcatcggt cagcccgcgc
      181 agcccggccg gatcggccgc cggccccaca atgaggaagt cgttgtgcat caccagccgg
      241 cgccgcagca ggtaacccga gtcggcgagg gcgcgctcgg cttcgggagc gtgcgacagc
      301 accacgtccg cgtcgccgcg acgccccagg gcgagcgcct gcccactccc cacagcgacc
      361 accttcacct ggtatcccgt ctcccgctcg aacaccggca gcagggagtc gagcaacccg
      421 gcgtcgcgtg tcgaggtggt cgtggccaga aggatttccc tggactggag gtcggtggag
      481 gacggtggaa gacggtggaa gacggtggag accgtgacga cgaagagggt ggccgcggtc
      541 cacggcactc gggttgcgct catatattga agcaacctaa cccaccgatc acttcatgag
      601 cgacctgctc gacgccatct ccggcgttcc caacgcgagc gagccgattc ccgggctcgt
      661 caccggcggc caacccctgt cccgtcacct gacggccctg aagcaggcgg gctgcgagct
      721 ggtgctggac tcccgcgacc cgatggagcc gaggcctatt gccgagccgg aggacgccgc
      781 agcggcggga ctcgagtacg tgtgcgtccc catgggacat acgcggggcg aagatgcgac
      841 gctgtcgaag atccgcgagg tcctgcgggc caacgtcggc aaacggaaaa tgttctacca
      901 ctgcgccagc gggaaccggg tcggcgcttc ggtcatcccc tacctgatgc tcgatcaggg
      961 attcggcgaa gaggatgcgg tgaatgcggc gcttcggatg gggatgcgga atccagaact
     1021 gttagaatgg gctttggact acgtgaagcg aaatgccagt aagtagtcgg acagagagac
     1081 gttcagtgac tcaggcgcgt tgcggttctc ggtcaggagt cactcaggaa aggcctctcc
     1141 tgtcggctac ggactgacaa ccgctgtgcg cctgagcgtc tgagcgcctg tctgtccagc
     1201 tgtccgacta tggtttcggc tcctctctgc ctcccgcata tctgtgccag tacatccgca
     1261 tctcgctgat cttgccgtcc ttggtctcgc agaagatcgc cccccgggca tccacccatt
     1321 ccccgctgct gcgacggcgg aacgtgcagc gaaactcggt ggcgaaccag gggcccactt
     1381 cgaagatctc gcccgaacgg aacgacacgt cggcctgggt gagggggagg tcctgccagt
     1441 aggcgcggat ggccgcctgc ccgatgtcct tctggctgaa gggcgtttcc acgaacacgg
     1501 cgtcgggggt gaacaccgag cagatgcgat ctacgtcggc ctgctcccac ccctcgccga
     1561 atgcgtcgat caggtgccgg taggccggga tcgctgacat gggcgccaaa tataccgaaa
     1621 tccggaaccg tgggcgccgg cgtacgtaca acggagtgtc ccggtcggga cattccggtg
     1681 gaagactcca acgcggagga acgatccatg ctgaagcgca tcctggcctc ggccgccgta
     1741 ctcgggctgc tggccctgcc cctctcggcc caggcgacac cgcaggccaa cgccggcaac
     1801 aaggtcacgc tcaccggtca ggtgatcgac gtgaactgct acacgacgca acatatgcag
     1861 ggcgcggcgc acaaggcctg cgcgacggcc tgtgccaaca agggcgtggc gctggcgatc
     1921 ctcgcgagcg acggcacgat ctacatgccc gtgagctcca agccgggcga tccgcagaat
     1981 ccccgcctga tgcagttcgt cgagggcaag gtcaaggtga ccggcacgca ccgatcggtg
     2041 agcggcctgc acaccatcga gatcgagacg gtcgaagcgg caagctgacc cctcacgcga
     2101 tgggtcgcgc gctgggcgcc gtcctgtgct tatgggcggc gtcggcctgc cgctccacgg
     2161 cgcccagtgg cctcaccctg ctgttctaca atcgctcacc cgcggccgtt ctgggcggac
     2221 gctcgtgggc gcccgatgcg ggcagctccc gcgtgctcgc cttcgacgac cgactgcacc
     2281 ccgtcaagtc actcggcggt gccgccctcg ccatgcccat ggccgtttcg accctgggca
     2341 accgcctcct cgtgaccgag gagacaggtg agggtgtggt gctcgatacc gcgggagagg
     2401 gagcgctggt gcgcgagtgg cccagcccct ttccgatcgc actgtacgcg gccgcgggca
     2461 gccacatcgt cgccgcccga tctccctacc gtgtcccttc gctgaccccg gaggcgccgg
     2521 acgccccgct ggtcagggtg ctggacacgc tggggcggcc gctcgagggg ctcgccgcga
     2581 tccacgtccc cgcgacgccg tttctcaccc agatcacgaa cgccggtgcg atcgccgtcg
     2641 attccggcgg cgccgtctac tacgcaccgc tggtgcgcga cgaaatcgtg aagttcggcg
     2701 cgagcggcga gcggcgctgg accaccacgc ggaacttgcg cccgtcagcg tcggaccccg
     2761 tctacctgcc ggcgcaggga ggcgggcacg acctgctgat cgagcaggcg accgtgaacg
     2821 ttgctctggc actcgggccc gacgggcggc tgtatgcact cggcgcggaa gattccgcgg
     2881 cgacgcaact gcgcgtggat gtgctcgaca cggcgaccgg cgcgatcctg gccacgcgcc
     2941 ggctcggggc aaacgagacc gccgttgccg tggacggcgg aggccggatc gcgaccttcg
     3001 acgcggccgt cctggcgcgc ggcgttcccg ctgcccggca gccgttcgcc ccggggttcg
     3061 cgcttcccga caccgcgggc gacacggtga ctctggcccg attcgcaggg aaggtgacgc
     3121 tggtcgattt ctgggcctcc tggtgcgatc cttgccgcga ggagtttccg cacatggtcg
     3181 cgctatacga ccggctggat cgcgccgatt tcgagatcgt ggcgatctcg gacgacgtgg
     3241 atcgcggcag catgctcgcc ttcatccgcg agttccatcc tccgtttccg gtgctggtgg
     3301 gtggcggacg gatgaaacag caataccact accg
//
LOCUS       DBNGZW010000184         2324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1108609, whole genome shotgun sequence.
ACCESSION   DBNGZW010000184 DBNGZW010000000
VERSION     DBNGZW010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1108609"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..556)
                     /locus_tag="AC16JC_04480"
     CDS             complement(<1..556)
                     /locus_tag="AC16JC_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR5163987.1"
                     /translation="MATTTTTVSLERGAIGLPEVLFQSITHMAPAVATALSIGAATGL
                     AGGITPLAVVFALIACLFTAYSIGQLAAHLPSAGGMYTYVSRGLGSFFGWLMAWAFTL
                     AEPLVPPALYASFGLFGAALITTLTGFSADLLWLPLAVLCGLVVWWLVYRGIALSTRV
                     GIGLGLIEIGIFVLVSALLVINAGS"
     gene            774..>2324
                     /locus_tag="AC16JC_04485"
     CDS             774..>2324
                     /locus_tag="AC16JC_04485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="apiosidase-like domain-containing protein"
                     /protein_id="HIR5163988.1"
                     /translation="MGSFRLARRVGCVALAVALLATASGAWTGHDAASATVLTAWRAR
                     VGASGVNGMATIQPAVAGTGSLVLQLLRLPASVSVFVTVRKGTCASAGSLLAAPPAFR
                     SSSTGGGVRTIALTKAQVTAMKAAMTTTGKVAIRVRVGSTTRCGAFARLGQPTGFLTR
                     QGAELVLVGRSFHELGFNKYDLTQQFVVSPEGAWGDNGPNSVAAGELALQVLQKHGFR
                     VVRTSVSPYYPAWFEQAFFDRDPARQAQKRREYFAGFDAMLDACDRHGIRIVASLIWN
                     VGNVAGLGPNSMHEGIAVRTSPGRARVEDFVRAVVTRYRDRPTIAMWEIGNEFNLLAD
                     LQNPDPMRNYTSDELATFYWDIATIIRTIDTEHLITTGSSAPRPAAMHLLTAARAGAP
                     IDWTPDTPQQLTDYLRLINPAPIDVISIHYAEDGMTSFGGVMGSPANLRFFAEASLAL
                     DKPLFVGEIGYSPDVRRYDSPVGLGMLRATLPVLVELKLPLTLYWMFNDDRGDPGREK
                     ELDLRYGRT"
BASE COUNT          400 a          804 c          770 g          350 t
ORIGIN      
        1 gtgatccggc gttgatcacc agcagcgccg agacgaggac gaaaatcccg atctcgatca
       61 gtcccaggcc gatcccgacc cgcgtcgaca gggcgatccc gcggtagacc agccaccaca
      121 cgacgagccc gcagagcacc gccagcggca gccacaggag gtcggcgctg aaccccgtga
      181 gcgtcgtgat cagggccgcc ccgaacagcc cgaacgacgc gtagagcgcg ggcgggacga
      241 gcggctcggc cagggtgaac gcccaggcca tcaaccagcc gaagaacgaa ccgaggcctc
      301 ggctgacgta cgtgtacatg ccgccggcag aaggcaggtg ggccgccagc tggccgatcg
      361 aataggccgt gaacaggcag gcaatcagcg cgaacaccac cgcgaggggg gtgatcccgc
      421 cggccagacc ggtcgccgcc ccgatcgaca gggccgttgc gacggcgggg gccatgtggg
      481 tgatcgactg gaagagcact tcgggcagcc cgatcgctcc tcgctcgaga ctcaccgtcg
      541 tcgtggttgt ggccatgccg acctccgcat acgtcgggtc cccctccgcc tgggcagcgc
      601 ctcgcccgac cgtggctgga gtgtatgcaa acgaattcct gacgggaaga ggcctggcgg
      661 gcgtgactcg acatcccgcg gaccagccca ggaacgcgcg cggcggttca cgacgaacgg
      721 ctctggcgcg tgacgccccc gccgcccatc gtgcggcgat cgcggagtgg gcgatgggat
      781 ccttccgact ggcccggcgc gtcggttgcg tggcgctcgc cgtggcgttg ctggccacgg
      841 cgtcgggcgc ttggaccggg cacgatgccg cgtccgcaac ggtgttgacc gcctggcgtg
      901 cccgagtggg cgcctccggg gtcaacggca tggccacgat ccaacccgcg gtcgccggca
      961 ccggctcgct cgtcctgcag ctgctccggc tgccggcgtc ggtgtccgtg ttcgtgaccg
     1021 ttcgcaaggg aacgtgtgcg agtgctggtt cgcttctggc ggcgccgccc gcgttcagaa
     1081 gctcgagcac gggcggtggc gtccgcacga tcgcactgac gaaggcccag gtcaccgcca
     1141 tgaaggccgc gatgaccaca accggcaagg tcgccatccg cgtcagggtc ggatccacca
     1201 ccaggtgcgg cgccttcgcg cgactcgggc agccgacagg attcctgacc cggcagggtg
     1261 ccgagctcgt gttagtgggc cggtcgttcc acgagcttgg cttcaacaag tacgacctga
     1321 cccagcagtt cgtggtgtcg cccgaagggg cctggggcga taacggtccg aacagcgtgg
     1381 ccgccggcga gctggcgttg caggtgctcc agaagcacgg gtttcgggtc gtccgaacca
     1441 gcgtctcgcc ctactatccg gcctggttcg agcaggcctt cttcgaccgc gatcccgccc
     1501 gtcaggcgca gaagcgtcgc gagtactttg ccggcttcga cgccatgctc gacgcgtgcg
     1561 accgacacgg aatccgcatc gttgccagcc tgatctggaa cgtgggcaat gtcgccggcc
     1621 tcggcccgaa ctccatgcac gaggggatcg cagttcgcac ctcgcccggc cgtgcgcgag
     1681 tcgaggactt cgtacgagcg gtcgtgactc gttatcgcga tcgcccgacc atcgcgatgt
     1741 gggagatcgg caacgagttc aatctcctgg ccgatctgca gaaccccgac ccgatgcgga
     1801 actacacgtc ggacgagctg gcgacgttct actgggacat agcgaccatc atccggacta
     1861 tcgacacgga gcacctcatc accaccggca gttcggctcc acgtcccgca gccatgcacc
     1921 tgctgacagc ggcgcgcgca ggcgcaccga tcgactggac cccagacacg ccgcaacagc
     1981 tgaccgacta cctccggctg atcaatcccg ctccgattga cgtgatcagc atccattacg
     2041 ccgaggatgg catgacctcc ttcggcgggg tgatgggcag cccggccaac ctgcggttct
     2101 ttgccgaggc cagcctggca ctcgacaagc cgctcttcgt cggcgagatc ggctacagcc
     2161 ccgacgtccg acgctacgac tcgccggtcg gcctcggcat gttgagagcc accctacccg
     2221 ttctggtcga gctgaagctc ccgctcaccc tgtactggat gttcaacgat gaccgaggcg
     2281 acccggggag ggagaaggaa cttgacctcc ggtacggcag aacc
//
LOCUS       DBNGZW010000185         8037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_834467, whole genome shotgun sequence.
ACCESSION   DBNGZW010000185 DBNGZW010000000
VERSION     DBNGZW010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8037
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_834467"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..461
                     /locus_tag="AC16JC_04490"
     CDS             <1..461
                     /locus_tag="AC16JC_04490"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013835.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA-guanine transglycosylase"
                     /protein_id="HIR5163989.1"
                     /translation="PKPLMYRTLEDLEPRLPAPLRRYLMGVGFPEDLVAAVERGVDVF
                     DCVAPTRMGRNGTAFTPGGPLNIRNAAFRTDSGPLDATCDCETCTTYSRGYLRHLFAA
                     GELLGLRLLSLHNVRYLIRLAGEMRAAIRSGEFTPWAADWRLRYLHPEPA"
     gene            458..787
                     /gene="yajC"
                     /locus_tag="AC16JC_04495"
     CDS             458..787
                     /gene="yajC"
                     /locus_tag="AC16JC_04495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HIR5163990.1"
                     /translation="MTPPLHALLFAPSGQAGPGMSVLLIQVAAIFGIFYLFIIRPQRK
                     QRQEHEKLLASLARGDQVITSGGIIGEVVHLKDNEVTIRSGEAKLVVLRNNIATVRNR
                     EEAAKPA"
     gene            784..1332
                     /gene="def"
                     /locus_tag="AC16JC_04500"
     CDS             784..1332
                     /gene="def"
                     /locus_tag="AC16JC_04500"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007675039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR5163991.1"
                     /translation="MSLRPLHLLGSPVLRQRAEPVATVDAEVRQLVEDLFDVMYASKG
                     VGLAANQVGIARRVAVVDVGKDSPEPLVLVNPVIVSRAAATETEEEGCLSIPEIFGDV
                     ERPASIVVEALDRDGTPYRLEATGYQARAIQHEIDHLDGVLFLDHLSAVKRGLLLSKW
                     KRLRKGETGYLKEVTPEPAGEL"
     gene            1332..2282
                     /gene="fmt"
                     /locus_tag="AC16JC_04505"
     CDS             1332..2282
                     /gene="fmt"
                     /locus_tag="AC16JC_04505"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR5163992.1"
                     /translation="MVIVFFGTPEFAVPSLGALVGEGFDVRAVVTQPDRPQGRSRSTV
                     VAPPVKVAALDEGLDVLQPERPSDPEFLHQLRTLAPDVGVVVAYGHILRPELLAIPRR
                     GMVNVHPSLLPELRGAAPVEWAILNGDETTGVTIMQLDAGMDSGPILHQIPHHIEPGI
                     TGGELSAHLAEMGAQALIEALALWEQGGLKLVPQDHTRATHAPKLTRETGRIDWTQPA
                     LTAARRTWALDPKPGAWATLASPAGSGERGAVEVKVFGGHVVEREGSPGEVLQADTRL
                     LIACGEQALEVDEVQPAGKDRMPAADWIHGRGVSAGQRFA"
     gene            2279..2899
                     /locus_tag="AC16JC_04510"
     CDS             2279..2899
                     /locus_tag="AC16JC_04510"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009229 - thiamine diphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR5163993.1"
                     /translation="MRAPLPRVHAVTDERIARRADLEQTAARLAAAGENVAVHARGHA
                     LSGLEHYRLAIRLSAYPPIRLFVNDRLDVALAAGAAGVQLAADSLPVDAARRLGPQWW
                     IGRSVHSVDQARSAQAEGADYLLAGPVFETATHPGATPLGVAGLRAIVELGHPVIAIG
                     GIDPGRAPDLARIGVYGVAAIRALWDAPEPAAAGRAFLEALERERK"
     gene            2896..3099
                     /gene="thiS"
                     /locus_tag="AC16JC_04515"
     CDS             2896..3099
                     /gene="thiS"
                     /locus_tag="AC16JC_04515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HIR5163994.1"
                     /translation="MIEVVVNGERRAVPADATLLDLLGVLGLDPRIVVVEHNRAIVRR
                     PELAQRRLSPDDAIELVHFVGGG"
     gene            3102..3899
                     /locus_tag="AC16JC_04520"
     CDS             3102..3899
                     /locus_tag="AC16JC_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HIR5163995.1"
                     /translation="MTTAVELVKDAPFTIGGRTFQSRLMVGTGKYRSNEEMVRAIEAS
                     GAEIVTVAVRRVDLDRSRDASILHHLDPKRLFLLANTAGCFSAADAIRYARLAREAGF
                     NEFVKLEVIGDQETLLPDVAGLLEATTVLVREGFKVLAYSNEDLITALRLEEAGAAAV
                     MPLASPIGSGLGLLNPFSVRTIKRRLHVPVVVDAGVGTASDACLTMEQGVDGILMNTA
                     LAAAQDPVAMAHAMRLAVEAGRAAFLAGRMPRREVAMPSSPLEGMLD"
     gene            3895..5175
                     /locus_tag="AC16JC_04525"
     CDS             3895..5175
                     /locus_tag="AC16JC_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013362.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR5163996.1"
                     /translation="MKGPTAARRAALDILRAVGAGAPFSDARDRAVRALPERDRRLAH
                     ELSAGVLRWRRTLDDALDLRHTDPRLHDILRLGAYQLRRLERVPAHAAVSTSVELARE
                     TAGEPAARYVNQALRRLATAGSGERGAVASHPDWLLARWRQQFGAEEAGRLVEWNDRK
                     PSLVVQPARTGLTTLRSSFEAAGFGVQEAPFGAGFAISRTPTAPRAPLPQSLPGYSTG
                     AFIVQDPAAALFCRFAAVPPGARLYDACAAPGGKAVALALGGARVTAGEWRRLRLGRL
                     RETVGRTGVAVPIVAADLRGAPFRPGTWDAVLLDAPCSATGTMARHPDARWRLSAAAI
                     TGLARRQAALLEAAAGLVRAGGVLIYAACSLEPEENERQVEAFLERHPEFARSTVCES
                     VPATLLSAAGDFRSLPQRHGIDGAYAARLVRETR"
     gene            5172..5969
                     /locus_tag="AC16JC_04530"
     CDS             5172..5969
                     /locus_tag="AC16JC_04530"
                     /inference="COORDINATES: protein motif:HMM:NF015734.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HIR5163997.1"
                     /translation="MKLRRHMPPLKDWHFPRWRGLRRLPPITQDQALRWAFWLGVAAV
                     AGYLIAALLVFPAPIFPHHQQVPRVLGLPLDEARRVLTGSRLGTIDNGSATHPSAAVG
                     TVIWQDPPPGVEAPANLKVALVTSAGPPRVPVPDVIGLSAPLATRLIAAAGLTVSRVE
                     SVQAAAPTGQTMLTRPAAATALVPGSGVGLVVSRGAPTITVPDLLGLSPADARARLEA
                     AGLVIGGVTRQRTANVAPGTVVAQRPAAGTLAAPGTLVDVVVARSPE"
     gene            5966..6643
                     /gene="rpe"
                     /locus_tag="AC16JC_04535"
     CDS             5966..6643
                     /gene="rpe"
                     /locus_tag="AC16JC_04535"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019424873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR5163998.1"
                     /translation="MSEATGIRIAPSVLSADLARLAEQVRAVLDGGADWLHVDVMDGR
                     FVPNLTFGANMVAALRKLTDRPIDVHLMVVEPERYIDPMADAGASVCTFHPEATVHVQ
                     RHLAAIRQRGMLAGLALNPASPLALVEDVVTDLDLLLIMMVNPGFGGQRYLPGSTDKI
                     RRARALLRARRSPAFLEVDGGIGPETIAEAHEAGADTFVAGHAIFAGADPAREVRELR
                     RRCAVEV"
     gene            6643..7176
                     /locus_tag="AC16JC_04540"
     CDS             6643..7176
                     /locus_tag="AC16JC_04540"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR5163999.1"
                     /translation="MSRRRLMLLGLGFVAVLAGALVIRLMPEIALVGVGSRAPAFQAI
                     DLRTGRPARFADYRGQVVLLNIWATYCIPCRTEMPAIERLSHRLAETDFHVLAVSVDV
                     VDSTAVDAFVRQLGLTFDVWHDQPGITQRLYQTTGVPETFVIDRHGVIVKKAIGAIDW
                     DDPVNEMLIRRLLADAS"
     gene            7173..7706
                     /locus_tag="AC16JC_04545"
     CDS             7173..7706
                     /locus_tag="AC16JC_04545"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HIR5164000.1"
                     /translation="MNRRRLTLFAGLAMAAVFVAALVIRILPQIALVGVGSAAPEFRA
                     VDLHTGRPARLADYRGKVLLINIWATWCQPCRVEMPAMERLWSRFKGSDFRILAVSVD
                     TDDSSAVSTFARDLGLTFDILHDKSGEIQQAYQTTGVPESYIIDRHGVIVKKAIGAIE
                     WDAPVNDVLIRRLLDDR"
     gene            7696..>8037
                     /locus_tag="AC16JC_04550"
     CDS             7696..>8037
                     /locus_tag="AC16JC_04550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164001.1"
                     /translation="MTADVRRGPFRNWGLKLAALFLSLMLYVFVAAQEQVTQELPMKL
                     EVRVPPGRVLLDAPKELTVVLRGKITELLRLRLFRQVIPLQVPDTLSTAFWSSPVLPG
                     SVDLPKGADVQV"
BASE COUNT         1102 a         2840 c         2871 g         1224 t
ORIGIN      
        1 aacccaagcc cctgatgtac cgcacgctgg aagacctgga gccaaggctg ccggctcccc
       61 tgcgccgtta tcttatgggc gtgggatttc ccgaggatct ggtggcggcg gtcgagcgcg
      121 gcgtggatgt gttcgactgc gtggcgccga cgcggatggg acgcaacggc acggcgttca
      181 caccgggcgg cccgctgaac atccgcaacg cggcgttccg gaccgattcc gggccgctcg
      241 atgccacgtg cgactgcgag acgtgcacca cgtactcgcg gggatacctg cggcacctgt
      301 tcgccgccgg agagctgctg ggattgcgcc tgttgtcgct gcacaacgtc cgttacctga
      361 tccggctcgc cggcgagatg cgcgcggcga tccgctcggg cgagttcacc ccgtgggccg
      421 ccgactggcg cctccgctac ctccacccgg aacccgcatg acccccccac tccacgcgct
      481 gctcttcgcc ccgtcgggac aggccggccc cggcatgagc gtcctgctca tccaggtcgc
      541 cgccatcttc gggatcttct atctcttcat catccgcccg cagcgcaagc agcgccagga
      601 gcacgagaaa ctgctcgcct cgctcgcgag gggcgaccag gtgatcacct cgggtggtat
      661 catcggggag gtcgtgcacc tcaaggacaa cgaggtcacg atccgcagcg gggaagcgaa
      721 actggtcgtc ctgcgcaaca acatcgccac ggtgagaaac cgcgaagagg cggcgaaacc
      781 cgcgtgagcc tgcgcccgct ccacctgctc ggctctcccg tgttgcgcca gcgcgccgaa
      841 ccggtcgcga cggtggacgc cgaggtgcgc cagctggtgg aggacctttt cgacgtcatg
      901 tacgcctcca agggcgtggg gctggccgcc aaccaggtgg gcatcgcgcg ccgggtcgcc
      961 gtggtggacg tgggcaagga ttcgcccgag ccgctggtgc tcgtgaatcc ggtgatcgtc
     1021 tcccgcgccg cggcgacgga gaccgaggaa gaaggctgcc tgtccatccc cgagatcttc
     1081 ggcgacgtcg agcgcccggc ctccatcgtg gtcgaggcgc tcgatcgcga cggcacaccg
     1141 taccgcctcg aggccaccgg ctaccaggcc cgcgccatcc agcacgagat cgatcacctg
     1201 gacggtgtgc tgttcctgga ccacctctcg gccgtgaaac gggggttgct gctgtccaag
     1261 tggaagcgcc tccgcaaggg cgaaactggc tacctgaagg aggtgacgcc ggagcctgcc
     1321 ggcgagctct agtggtcatc gtcttcttcg ggacgcccga gttcgccgtc ccctcgctcg
     1381 gtgccctggt gggtgaaggg ttcgacgtcc gggccgtggt cacgcagccc gatcgtccgc
     1441 agggccgctc ccgctccacc gtggtcgcgc cccccgtcaa agtcgccgcg ctggacgaag
     1501 ggctcgacgt gctgcagccc gagcggccct ccgatccaga gttcctgcac cagctgcgaa
     1561 ccctggcccc agacgtgggc gtggtcgtgg cgtacggtca catcctccgc cccgagctgc
     1621 tggccatccc gcggcgcggc atggtgaacg tgcacccctc gctgcttccc gagctgcggg
     1681 gcgcggcgcc ggtcgaatgg gccatcctga acggcgacga aacgaccggc gtcacgatca
     1741 tgcagctcga tgcggggatg gactccggtc ccatcctgca ccagatccct caccacatcg
     1801 agcccggcat tacgggcggg gagctgtcgg cgcacctggc cgagatggga gcgcaggccc
     1861 tgatcgaagc gctcgccctg tgggagcagg gcggactcaa actcgtccca caggaccaca
     1921 cccgcgccac ccacgctccg aagctcaccc gggagacggg gcgcatcgat tggacgcagc
     1981 cggccctcac ggcggcgcgc cgcacgtggg cgctggaccc gaagcccggc gcctgggcga
     2041 ccctcgcttc gcctgcggga agcggggagc ggggagcggt cgaggtgaaa gtcttcggcg
     2101 gccacgtagt ggagcgcgag ggttccccgg gcgaagtgtt gcaggccgac acccggttgc
     2161 tgatcgcctg cggcgagcag gcgttggagg tggacgaggt ccagcccgcg ggtaaggatc
     2221 gcatgccggc ggccgactgg atccacggcc gaggggtgag cgccgggcag cgattcgcgt
     2281 gagggcgccc cttccccggg tgcacgcggt caccgacgag cgcatcgccc gtcgggccga
     2341 tctcgagcag accgccgccc ggctcgccgc tgccggtgag aacgtggcgg tgcatgcacg
     2401 cggacatgct ctctccggcc tcgagcatta tcgcctggcc atccgcctat ccgcctatcc
     2461 gccgatccgc ctgttcgtca acgaccgcct cgacgtggcc ctggccgcgg gagcggccgg
     2521 cgtacagctc gccgcggaca gcctcccggt cgatgccgcc cgcaggctgg ggccgcagtg
     2581 gtggatcggc cggtccgtgc actccgtgga tcaggcgcgg tccgcgcagg cggagggagc
     2641 cgactatctt ctggcagggc cggtgttcga gaccgccaca caccccgggg cgacgccgct
     2701 cggcgtcgca gggctgcgcg cgatcgtcga gctcggacac ccggtgatcg cgatcggagg
     2761 catcgacccc gggcgtgcgc ccgacctggc ccggatcggg gtatacggcg tcgcggcgat
     2821 ccgcgccctg tgggacgccc ccgagccggc cgccgcgggc cgcgcgtttc tggaagcgct
     2881 ggagcgggag aggaaatgat cgaagtcgtc gtcaacggcg aacgccgagc ggtgccggcc
     2941 gacgccacgc tgctcgacct gctcggggtc ctgggactcg acccccgcat cgtcgtggtg
     3001 gagcacaacc gggcgatcgt tcgacggccg gagctcgccc agcggcgcct gtcgccggac
     3061 gacgcgatcg agctggttca cttcgtggga ggcgggtgag catgacgacc gccgtggagc
     3121 tcgtgaagga cgcgccgttc acgatcggcg gacggacgtt ccagtcccgc ctcatggtcg
     3181 gcaccgggaa gtaccgcagc aacgaggaga tggtgcgggc gatcgaggcg agcggcgccg
     3241 agatcgtcac cgtcgccgtg cggcgggtgg acctggaccg gtcccgcgac gcgagcatcc
     3301 tccaccatct cgaccccaag cgcctcttcc tgctggccaa caccgccggc tgcttttccg
     3361 cggcggacgc gattcgctac gcgcggctcg cccgcgaggc gggcttcaac gagttcgtga
     3421 agctcgaggt gatcggcgat caggagaccc tgttgccgga cgtggcgggg ctgctcgagg
     3481 ccaccaccgt gctggtgcgc gagggattca aggtcctcgc ctacagcaac gaagacctga
     3541 tcaccgcgct gcgtctggag gaggccggcg cggcggcggt gatgccgctc gcttcgccga
     3601 tcggcagcgg cctcggcctg ctgaacccgt tcagcgtccg caccatcaag cgccggctcc
     3661 atgttcccgt cgtggtggac gcgggagtcg gcaccgcctc ggatgcctgc ctcaccatgg
     3721 agcagggcgt ggacggcatc ctcatgaaca ccgcgctggc cgccgcgcag gacccggtcg
     3781 ccatggcgca cgccatgcgc ctcgcggtcg aggcgggacg ggccgcgttt ctggcgggac
     3841 ggatgccgcg gcgcgaagtc gccatgccgt cgtcaccgct cgaggggatg ctggattaag
     3901 gggccgactg ccgcgcgccg cgcggctctc gacattctgc gcgccgtcgg tgccggggcg
     3961 ccgttctcgg acgcgcgcga tcgcgcggtg cgcgcgttgc cggaacgcga ccggcgcctg
     4021 gcccacgagc tgtcggccgg tgtgttgcgc tggcggcgca cgctcgacga cgccctcgat
     4081 ctccgccaca ccgatcctcg gttgcacgac atcctgcggc tgggtgccta ccagctgcgc
     4141 cgcctggagc gggtccccgc ccacgccgcc gtctcgacca gcgtggagct ggcccgcgag
     4201 acggcggggg agccggcggc gcgctacgtg aatcaggcgc tgcggagact cgcaacggcg
     4261 gggagcgggg agcggggagc ggtcgcttcg catcccgact ggctcctcgc ccgatggcgg
     4321 cagcagttcg gcgccgaaga agcaggccgc ctcgtcgagt ggaacgaccg gaagccgtcg
     4381 ctcgtcgtgc agccagccag gactggtctc acgactttga ggtccagctt cgaggctgcc
     4441 gggttcggag tccaggaagc gccctttggc gcgggattcg ccatctcccg aactccgacc
     4501 gctccccgtg ccccgctccc ccagagcctc ccggggtatt ccaccggcgc ctttatcgtt
     4561 caagatcctg cggccgccct cttctgccgc ttcgcagcgg tgccgcccgg tgcgagattg
     4621 tacgacgctt gcgccgcccc cgggggcaaa gcagtggcgc tcgccttggg cggagcgcgg
     4681 gttaccgcgg gggagtggcg tcgcctgcgg ttggggcgcc tgcgtgagac ggtaggacgg
     4741 accggagtag cggtcccgat cgtcgcagcg gatcttcgcg gcgcgccgtt tcggcccggg
     4801 acctgggacg cggtgctgct cgacgcgccg tgcagcgcga ccggcacgat ggcgcggcat
     4861 cccgacgcgc gctggcggct ctcggccgcg gcaatcaccg gcctcgcgcg gcgccaggcg
     4921 gcgctgctcg aggcggcggc cgggctggtg cgggcgggcg gcgtgctgat ctacgccgcg
     4981 tgctccctcg aaccggaaga gaacgaacga caggtggagg cgttcctgga gcgtcacccc
     5041 gagtttgcgc ggtccacggt gtgcgagtcg gtcccggcga cgctgctgag cgcggccggg
     5101 gacttccgct cgctccccca gcggcacggc attgacggcg cgtacgcggc gcgtctggtg
     5161 cgggagactc gatgaagctc cggcgtcaca tgccgccgct caaggactgg cacttccccc
     5221 ggtggcgcgg attgcgccgc ctgccgccca tcacacaaga ccaggcactg cgttgggcgt
     5281 tctggctcgg tgtggctgcg gtggccggct acctgatcgc ggcgctgctc gtgttccccg
     5341 caccgatctt cccgcaccat cagcaggtcc cgcgcgtact cggcctgccg ctcgacgagg
     5401 cgcggcgcgt cctcacgggc tcgcggctcg gcaccatcga caacgggtcc gcgacgcacc
     5461 cgtcagccgc cgtggggacc gtgatctggc aggatccgcc accgggggtc gaggcgcccg
     5521 ccaacctgaa ggtggcgctc gtgacgagcg ccggaccgcc gcgcgtcccg gtgccggacg
     5581 tcatcggact gagcgccccg ctcgccacgc ggttgatcgc ggcggcgggg ctcacggtga
     5641 gccgggtgga atcggtgcag gcggccgccc ccaccgggca gacgatgctc acgcgtcctg
     5701 ccgccgccac cgcgctcgtt ccgggtagcg gcgtcggcct cgtcgtcagt cgcggcgccc
     5761 ccacgatcac cgtccccgat ctgctgggac tctctcccgc ggacgcacgg gcgcggctcg
     5821 aggccgccgg actcgtcatc ggcggcgtga cgcggcagcg caccgccaac gtcgcgcccg
     5881 ggacggtggt ggcgcagcgg ccggccgccg ggacgctcgc cgcccccggc acgctggtgg
     5941 acgtggtggt cgcccggagc ccggaatgag cgaggccacg ggtatccgca tcgcgccgag
     6001 cgtgctgtcc gccgacctcg cgcggctcgc cgagcaggtg cgcgcggtgc tggacggcgg
     6061 cgccgactgg ctccacgtgg acgtcatgga tggacggttc gtcccgaatc tcacgttcgg
     6121 cgccaacatg gtggcggcgc tgcgcaagct caccgaccgg ccgatcgacg tgcacctcat
     6181 ggtggtcgag ccggagcgct acatcgaccc gatggcggac gcgggcgcgt cggtgtgtac
     6241 ctttcatccg gaggccacgg ttcacgtcca gcgtcacctc gcggcgatcc ggcagcgcgg
     6301 catgctcgcc gggctggccc tcaatcccgc gtcgccgctg gccctggtgg aggacgtcgt
     6361 gacggacctg gatctgctgc tcatcatgat ggtcaacccg gggttcggcg ggcagcgcta
     6421 cctgccgggg tccaccgaca agatccggcg cgcgcgcgcc ctgctgcggg cccgccggtc
     6481 gccggcgttt ctcgaggtgg acggcggcat cggccccgag accatcgcgg aggcccacga
     6541 ggccggggcc gacaccttcg tcgccggcca tgcgatcttc gccggcgccg acccggcccg
     6601 cgaagtgcgc gagctgcggc ggcgctgcgc ggtggaggtc tgatgagccg gcgccgtctg
     6661 atgctgctcg gcctggggtt cgtcgcggtg ctcgccgggg cgctcgtgat ccgcctgatg
     6721 cccgagatcg cgctcgtcgg cgtcggctcc cgggcccccg ccttccaggc gatcgacctc
     6781 cggaccggac gccccgcccg cttcgccgac taccgcggcc aggtcgtgtt gctcaacatc
     6841 tgggcgacgt actgcatccc ctgccgcacc gaaatgccgg cgatcgagcg gctgtcccac
     6901 cggctcgcgg agaccgactt ccatgtcctg gcggtgagcg tggatgtcgt cgattcgact
     6961 gcggtggacg ccttcgtccg ccagctgggc ctgacgttcg acgtatggca cgaccagccg
     7021 gggatcacgc agcggctgta ccagacgacc ggcgtccccg agacgttcgt gatcgaccgc
     7081 cacggcgtga tcgtgaagaa ggccatcggt gcgatcgact gggacgatcc ggtgaacgag
     7141 atgctgattc gccggctgct cgcggacgcc tcgtgaaccg gcgccggctg actctgttcg
     7201 ccgggctcgc gatggccgcg gtgttcgtgg ccgcgttggt gatccgcatt ctcccccaga
     7261 tcgcgctcgt cggcgtcgga tcggccgccc cggagttccg ggccgtagac ctgcataccg
     7321 gacgccctgc ccggctcgcc gactatcgcg ggaaggtgct gctgatcaac atctgggcga
     7381 cgtggtgcca gccgtgccgg gtcgaaatgc ccgcgatgga gcggttgtgg agtcgcttca
     7441 agggaagcga ctttcgcatc ctggcggtga gcgtcgatac cgacgattcg agcgccgtga
     7501 gcacgttcgc ccgggacctg gggctgacct tcgacatcct gcacgacaag tcgggggaga
     7561 tccagcaggc ctatcagacc accggcgtcc cggagagcta catcatcgat cgccacggcg
     7621 tcatcgtgaa gaaggcgatc ggtgccatcg aatgggatgc gcccgtcaac gacgtgctga
     7681 tccggcggct gctcgatgac cgctgacgtg cgccgcggcc cgttccggaa ctggggcctc
     7741 aagctggccg cgctgttcct gtccctgatg ctctacgtct tcgtggcggc gcaggagcag
     7801 gtcacgcagg agctgccgat gaagctcgag gtgcgcgtgc ctcccgggcg cgtgctgctc
     7861 gatgcaccga aggagctcac ggtggtgctg cgcggcaaga tcaccgagct cctgcggtta
     7921 cggctgtttc gtcaggtcat tccactgcag gtgccggata ccctgagcac cgccttctgg
     7981 agcagtccgg ttcttcccgg aagtgtcgac ctgcccaagg gcgcggatgt gcaggtg
//
LOCUS       DBNGZW010000186         5073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_768477, whole genome shotgun sequence.
ACCESSION   DBNGZW010000186 DBNGZW010000000
VERSION     DBNGZW010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5073
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_768477"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..668
                     /gene="nrfD"
                     /locus_tag="AC16JC_04555"
     CDS             <1..668
                     /gene="nrfD"
                     /locus_tag="AC16JC_04555"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR5164002.1"
                     /translation="FGSVKANPWWSSVLMPVVFLFSAIVSGIALVLIIYLVTTLFRSR
                     PVNMPCVDKLASFLFYAMIIDVSLEMLDFLHRLYESEESIGILSQMISSRLFLSLIVL
                     QIVIGTIVPLVLLGASSPALRRGGAARGTDELRRLIYLVAAVLVEIGIFSIRWNVVVG
                     GQLFSKSLRGLTVYKVEVLGAEGVAITFALLALPLVILWVLVKILPPWADAETRSAEA
                     TAH"
     gene            727..1377
                     /locus_tag="AC16JC_04560"
     CDS             727..1377
                     /locus_tag="AC16JC_04560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164003.1"
                     /translation="MRCAKWIGSWLTLGAVLAGCQPSQPAQRAGDSTAATGGLSRADY
                     MLLAAAKVALPPPGLAAGDLPDPSSREAQLVITYCGQCHGLPSPMAHSATDWPSVARR
                     MWLRMEMLPESLGIKTPTPSERGLILSYLLANSLKVSGAVLPAGKGRDEFAVVCNQCH
                     ALPDPRVHAADDWPAVYARMERNMERMQVKAPGGTVGTDILLYLQTVAGRRPAARQ"
     gene            1454..1939
                     /locus_tag="AC16JC_04565"
     CDS             1454..1939
                     /locus_tag="AC16JC_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR5164004.1"
                     /translation="MSAIIRKTPRDPMLDLLYNLERRMGRIFNEPVVFSDWSLPEMAS
                     TAWVPVVDVFEEPEHIRIVAEIPGVKPENFKISVEDNVLTISGTKEQVAEEKAEKVHR
                     YERTYGSFERSFTLPGTVDPASIKATYEHGVLSIMLPKLEKAKAREIKVDVAPAKALK
                     S"
     gene            complement(2018..3046)
                     /locus_tag="AC16JC_04570"
     CDS             complement(2018..3046)
                     /locus_tag="AC16JC_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013338968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Acg family FMN-binding oxidoreductase"
                     /protein_id="HIR5164005.1"
                     /translation="MPDILLEPYAEALDEFPITGTTQDKLRAAVRYAVRAPSSHNSQP
                     WRFQLVGDVLELRADRSRALPVVDPDDRELTISCGAALFHLCLALRHFGSAPQVERLP
                     NAADPDLLARVRVTGPWERTAEDVALFQAIPRRRTNRQPFEQRMLPGALGTALESAAQ
                     AEGAWLHVFRTPEARSAIAGLVAAGDRIQMADKRFRRELAAWLRPNWTDRRDGMPGFA
                     LGMGDVMAAAGPLIVRTFDVGRGRAAHDEELAEGSPLMALLGTPQDTPPHWLAAGEAL
                     AHLLLRAQVDGVSASYLNQPIEVPELRPRLQLVAGRPGYPQMLLRLGIGREAAETPRR
                     PVSDVLAG"
     gene            complement(3157..>5073)
                     /gene="acnA"
                     /locus_tag="AC16JC_04575"
     CDS             complement(3157..>5073)
                     /gene="acnA"
                     /locus_tag="AC16JC_04575"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015249709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR5164006.1"
                     /translation="GIATLPLADRATIGNMAPEYGATCGIFPVDAETLRYLELTGRAR
                     EQVALVEAYCKEQGLFQSADTPDAAYTDTLSLDLGSVEPSLAGPRRPQDRVSLAQVGK
                     TFLAALPTLIKPTSPIAAPPAANGGRWESEGGGTAVAVAAPPQRTHVSLKIEGEECRL
                     HHGSVVIAAITSCTNTSNPSVMIAAGLLAKKAVEKGLTSRPWVKTSLAPGSKVVTDYL
                     DQAGLTPYLEKLRFNLVGYGCTTCIGNSGPLPEAVSAAVTEGELVVAAVLSGNRNFEG
                     RIQQEVRANYLASPPLVVAYALAGRIDIDLQHDPLGTGKDGKPVFLRDVWPTTREVDE
                     VVRTSIKSAMFHKSYGEVFTGDERWRGLPVPAGDRFAWEPASTYIRRAPYFDGMPAAP
                     APIRDITAARVLALLGDSITTDHISPAGSIKKTSPAGQYLVGKGVQPIDFNSYGSRRG
                     NHEVMARGTFANVRLKNLLAPGTEGPVTRHLPDGKEMTIYDASVQYQADRTPLLVLAG
                     KEYGSGSSRDWAAKGPRLLGVRAVIAQSYERIHRSNLVGMGILPLQFLPDQGAQALGL
                     TGEEVFELKGLADVLAEGFPRGKELAVGAVKPDGSRVGFRVTVRIDTPQELQYYRHGG
                     ILEYVLRQLAGQRA"
BASE COUNT          772 a         1719 c         1770 g          812 t
ORIGIN      
        1 tcttcggatc ggtcaaggcg aacccctggt ggagcagcgt gctcatgccg gtggtgttcc
       61 tcttctccgc gatcgtgtcg gggatcgccc tggtgctgat catctacctg gtgaccacgc
      121 tgttccgctc gcgcccggtc aacatgccgt gcgtggacaa gctggccagt ttcctcttct
      181 acgcgatgat catcgacgtc tcgctggaga tgctggattt cctgcatcgc ctgtacgaat
      241 ccgaggagtc gatcggcatc ctctcgcaga tgatcagcag ccgcctgttc ctgagcctca
      301 tcgtgctcca gatcgtcatc gggaccatcg tcccgctcgt cctgctgggg gcgtcttcgc
      361 ccgcgctgcg gcgcggcggc gcggcccggg gaaccgacga gctgcggcgg ctgatctatc
      421 tcgtcgccgc cgtactcgtg gaaatcggga tcttcagcat ccggtggaac gtcgtggtcg
      481 gtggacagct tttctccaag agcctgcgcg gcctgacggt ctacaaggtg gaggtgctgg
      541 gagcggaagg tgtcgccatc acgttcgcgc tgctcgccct gccgctcgtg atcctctggg
      601 tgctggtcaa gatcctgcct ccctgggccg acgcggagac gcgtagcgcc gaagcaacgg
      661 cgcattagct ttcaccgccg tcgcaggcaa tatcagacct ggcacgtcgc actccccgga
      721 ggatccatgc gctgtgcgaa gtggattggc agctggctta ccctcggtgc cgtccttgca
      781 gggtgtcagc cctcccagcc tgcccagcgg gcgggcgaca gcacggccgc cacgggcggc
      841 ctcagccgcg cggactacat gctgctcgcc gccgccaagg tggcgctgcc cccgcccggc
      901 ctggccgccg gcgatctccc cgatccgtcg tcgcgtgagg cgcagctggt gatcacctac
      961 tgcgggcaat gccacgggtt gccctccccg atggcccaca gcgcgacgga ctggcccagc
     1021 gtggcccggc ggatgtggct gcgcatggag atgctgcccg agtcgctcgg gatcaagacg
     1081 ccgaccccgt ccgagcgcgg cctgattctg agctacctcc tggccaattc cctgaaggtc
     1141 agcggcgccg tgctgccggc gggcaaggga cgcgacgagt tcgccgtcgt gtgcaaccaa
     1201 tgccatgcgt tgcccgaccc gcgggtccat gccgccgacg attggccggc cgtgtacgcc
     1261 cggatggagc gcaacatgga gcggatgcag gtcaaggcgc cgggcggaac ggtcggtacc
     1321 gacatcctgc tgtacctgca aaccgtggcg gggaggcggc cggcggcgcg gcagtagccg
     1381 aatctcacgc tcgtttcaac ggcgattcac ggcgcggggc ctacgttgcg atcacggagg
     1441 gaggtgagac gcaatgtcgg cgattattcg caagaccccg cgtgatccga tgctcgatct
     1501 gctgtacaac ctcgagcggc ggatgggaag gatcttcaac gagccggtcg tgttcagcga
     1561 ctggtcgcta ccggagatgg ccagcacggc gtgggtgccg gtggtggacg tcttcgagga
     1621 gcccgagcac atccgcatcg tggccgagat ccccggcgtg aagccggaga atttcaagat
     1681 ttcggtggag gacaacgtcc tcaccatctc ggggaccaag gagcaggtgg ccgaggagaa
     1741 ggccgagaag gtgcaccgct atgagcggac ctacggctcg ttcgagcgca gcttcacgct
     1801 gccgggcacc gtcgatccgg cctcgatcaa ggcgacctat gagcacggcg tgctgagcat
     1861 catgctgccg aagctcgaga aggcgaaggc tcgcgagatc aaggtggacg tcgctccggc
     1921 aaaggccctg aagtcctgag cgccaaagcg gtaggaagcg aagcgacaac gccgggatcc
     1981 tttcggatcc cggcgttctt gctgccagca gcgaatgtca gccggcgagc acgtcggaca
     2041 cggggcggcg cggcgtttcc gcagcctccc ggccgatccc gagccggagc agcatctgcg
     2101 gatacccggg ccgtccggcg acgagctgca accgtggccg tagctcgggg acttcgatcg
     2161 gctggttcag ataggaggcg gacaccccgt ccacctgggc gcgcagcagc aggtgcgcca
     2221 gggcctcccc ggccgcgagc cagtgcggcg gcgtgtcttg cggcgtgccg agcaacgcca
     2281 tgagcggcga gccttcggcc agctcctcgt cgtgcgccgc ccgcccgcgg cccacgtcga
     2341 acgtccgcac gatgagcggc ccggcggcgg ccatcacgtc gcccatgccc agcgcgaagc
     2401 ccggcatgcc gtcgcgccgg tcggtccagt tcggccgcag ccaggccgcc agctcgcggc
     2461 ggaatcgctt gtccgccatc tggatccggt ctcctgccgc cacgaggccg gcgatcgcgc
     2521 tgcgtgcctc gggcgtccgg aacacgtgga gccacgcgcc ttcggcctgg gcggccgatt
     2581 cgagcgcggt cccgagcgcc cccggcagca tgcgctgctc aaacggctga cggttggtgc
     2641 ggcgccgcgg gatggcctgg aacagcgcga catcttccgc ggtccgttcc cacggcccgg
     2701 tcacccgcac gcgggccagc aggtccgggt cggccgcgtt cggcaggcgc tcgacctggg
     2761 gggcggagcc gaagtgccgc agcgcgaggc agaggtggaa cagggccgcg ccgcagctga
     2821 tggtcagctc ccggtcgtcg ggatcgacga cgggcagcgc gcgcgagcgg tcggcccgca
     2881 gctccagcac atcccccacc aactgaaagc gccacggctg gctgttgtga ctggaaggcg
     2941 cccgcacggc atagcgcacc gcggcgcgca gcttgtcctg cgtcgttccg gtgatcggga
     3001 actcgtccag cgcttccgca tagggctcga gcaggatatc gggcatggct gacgctccgc
     3061 agtggtccga atcgcaacct agaggtgcgg cgtgaagaat ccccaaagga agcctgaagg
     3121 ggaggctatc ccggtgggac gaatcatcag cttggtctag gcgcgctggc cggcgagctg
     3181 gcgcagcacg tactcgagga tccccccgtg ccggtagtac tgcaactcct gcggcgtgtc
     3241 gatgcggacc gtcacgcgga agccgacccg tgacccgtcg ggcttcacgg ccccgaccgc
     3301 gagctccttg ccgcgcggaa atccctcggc cagcacgtcg gccagcccct tcagctcgaa
     3361 cacttcttcg cccgtgagtc ccagcgcctg cgcgccctga tcgggcagga actgcagcgg
     3421 caggattccc atcccgacca ggttcgagcg gtgaatccgc tcgtagctct gggcgatcac
     3481 ggcccggacg ccgagcagcc gcgggccctt cgcggcccag tcgcgcgagg agccggagcc
     3541 gtattccttg cctgcgagca ccaggagcgg agtccggtcg gcctggtatt gcaccgacgc
     3601 gtcgtagatc gtcatctcct tcccgtcggg gaggtgccgc gtgacggggc cttccgttcc
     3661 cggcgcgagg agattcttga ggcggacgtt ggcaaacgtg ccgcgcgcca tcacctcgtg
     3721 attgccgcga cgcgagccgt aggaattgaa atcgatcggc tggacgccct tccccaccag
     3781 gtactggccg gcgggactcg tcttcttgat ggaaccggcg ggagagatgt gatcggtggt
     3841 gatcgagtcg cccagcaggg cgagcacgcg cgccgcggtg atgtcgcgga tcggcgcggg
     3901 ggcggccggc ataccgtcga agtacggtgc gcggcggatg taggtcgacg cgggctccca
     3961 cgcgaagcga tccccggcgg ggacgggcag cccgcgccag cgctcgtcac cggtgaacac
     4021 ctcgccgtag gacttgtgga acatcgccga cttgatcgag gtgcggacca cctcgtccac
     4081 ctcgcgcgtg gtgggccaga cgtcgcgcag gaagaccggc ttgccgtcct tgcccgtgcc
     4141 gagcgggtcg tgctgcagat cgatgtcgat ccggccggcg agcgcgtagg ccaccacgag
     4201 cggcggggag gcgaggtagt tcgcgcgcac ttcctgctgg atgcgccctt cgaagttacg
     4261 gttgcccgac agcaccgccg ccaccaccaa ctcgccctcg gtgacggccg ccgacacggc
     4321 ctcggggagc ggtccggaat tgccgatgca ggtggtgcag ccgtagccca cgagattgaa
     4381 gcggagcttc tcgaggtacg gcgtcagccc ggcctggtcg agatagtcgg tcaccacctt
     4441 ggagcccggc gcgaggctgg tcttcaccca cgggcggctg gtcagaccct tctcgaccgc
     4501 tttcttcgcg agcaggcccg cggcgatcat caccgacgga ttcgaggtgt tggtgcagct
     4561 cgtgatggcg gcgatgacga ccgacccgtg gtgcagccgg cactcctcgc cctcgatctt
     4621 cagcgacacg tgcgtgcgct ggggcggcgc cgcgaccgcg accgccgtgc cgcccccctc
     4681 gctctcccag cgtcctccgt tggcggcggg cggcgcggca atgggcgagg tcggcttaat
     4741 gagcgtcggc aacgccgcga gaaaggtctt gcccacctgg gcgaggctca cgcggtcctg
     4801 cggccggcgg ggcccggcga gactcggctc cacgctgccg aggtcgagtg acagggtgtc
     4861 ggtatatgcg gcgtccggcg tatccgccga ttgaaacagt ccctgctcct tgcagtaggc
     4921 ctcgaccagc gcgacctgct ccctggcacg gccggtgagc tccagatagc gcaatgtctc
     4981 ggcgtccacc gggaagatgc cgcaggtcgc gccgtactcc ggcgccatgt tgccgatcgt
     5041 ggcgcggtcg gcgagcggca gcgtggcgat ccc
//
LOCUS       DBNGZW010000187         2201 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_694235, whole genome shotgun sequence.
ACCESSION   DBNGZW010000187 DBNGZW010000000
VERSION     DBNGZW010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2201)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2201)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2201
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_694235"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="AC16JC_04580"
     CDS             <1..560
                     /locus_tag="AC16JC_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR5164007.1"
                     /translation="AALRPSPLREVPLDTISAEVPPYVEPSRSRQIDPGGLTVTGPAN
                     IDVPLIPAPGPIPGVPAVDVPLLGRPATPVEGPMVPEGEGSTGVLMASLVQDPPELLA
                     APVPPYPALLREARVEGQVLVQVVVDTTGRPEPGSLRVVESTNGGFNAAALGTIRGAL
                     FRPGRVWGRAVRVLVQVPVAFRLQP"
     gene            664..1341
                     /locus_tag="AC16JC_04585"
     CDS             664..1341
                     /locus_tag="AC16JC_04585"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR5164008.1"
                     /translation="MFDVLIESKKPSHKGRMAGTSLASLIFHTLVIGGAVYATLSAGP
                     ADDAIKVDTALVYIQPEQQKQPDQPPPPQLDVQFKGFQTVVAPTDIPTNIPPVNLQEH
                     FDPKDFSGVGVEGGVATGIVPSSDAVLSVDVVQEKPERLAGPLPVYPPLLSQAGIEGV
                     VMVQAIIDTMGRVEPNSLKIIQTANPGFNESAKQTVVKSLFRPARVYGKAVRVLIQIP
                     INYNLKH"
     gene            1383..2129
                     /locus_tag="AC16JC_04590"
     CDS             1383..2129
                     /locus_tag="AC16JC_04590"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR5164009.1"
                     /translation="MPDIIAMWSASTYFAKGIVVILVFMSVWSVSTFIMKWWQLRRST
                     RETVRFVPEFSRFLQEEHLDAAIALAEKTKKSHVARVLGEALAEVKPLLRDRATITAA
                     DINSAERAVERQMLIIVSELKRGLGILATIGATAPFVGLLGTTMGIVNAFTGMMASGG
                     GGSLGAVAGGISEALITTAIGLVAAIPAVWFYNYFTTKIDFISVEMTYTSKELIDYLI
                     RSVGSEFGRSIFTKEFQAQKATQSSGPISR"
BASE COUNT          361 a          705 c          758 g          377 t
ORIGIN      
        1 gcgcggcgct ccgtccctcg ccgctccgcg aagtcccgct cgacaccatc agtgccgagg
       61 tccctccgta cgtggagccc tcgaggtccc ggcagatcga cccggggggc ctgaccgtga
      121 cgggtcccgc caacatcgat gttccactga tcccggcgcc ggggccgatt cccggcgtgc
      181 ccgctgtcga tgtgccgctg ttgggtcgtc cggccacgcc cgtcgagggg cccatggtcc
      241 ccgaagggga ggggagcacg ggagtgttga tggcgtccct ggttcaggac ccgccggagc
      301 tcttggcggc gccggtgccg ccctatccgg cgctgctgcg ggaggcgaga gtggaagggc
      361 aggtcctggt ccaggtggtc gtagacacga ccggccggcc ggagccggga tcactgcggg
      421 tggtagaaag cacgaacggg gggttcaacg cagccgccct ggggacgatc cggggggcgc
      481 tgtttcggcc cggcagggtg tggggccggg ccgtgcgggt gctggtgcag gtgccggtgg
      541 cattccggtt gcagccctga gcgggtgact cacccggctg tcatcgtttt gtaatgatcc
      601 tcccactagc gggcaagccg gacccgatag tatctcggta gccatcgcga cggaggttgg
      661 accgtgttcg acgttctgat cgagtccaag aagcccagcc acaagggccg catggcgggg
      721 accagcctcg cctcgctgat cttccatacg ctggtcatcg gcggggccgt gtatgcgacc
      781 ctgtcggcgg gaccggcgga tgatgcgatc aaggtggata cggcgctggt gtacatccag
      841 cccgagcagc agaagcagcc cgaccagccg ccgccgccgc agctcgacgt ccagttcaag
      901 ggcttccaga ccgtcgtggc gccgaccgac atcccgacca acatcccgcc cgtgaatctg
      961 caggagcatt tcgatcccaa ggatttctcg ggcgtcggcg tcgagggcgg cgtcgccacc
     1021 ggtatcgtgc cgtcgagcga cgcggtgctc tccgtggacg tcgtgcagga gaagccggaa
     1081 cgcctggccg ggccgctgcc cgtctacccg ccgctcctgt cgcaggccgg gatcgaaggc
     1141 gtggtcatgg tgcaggcgat catcgacacc atgggacgcg tcgagccgaa ctcgctgaag
     1201 atcatccaga ccgccaaccc cggcttcaac gagtccgcca agcagacggt ggtgaagtcg
     1261 ctgttccgcc cggcgcgggt gtacggcaag gcagtgcgtg tcctgatcca gatcccgatc
     1321 aactacaacc tgaagcacta acccgctgca gcactcaccg acgcccggag gagccgcctc
     1381 ggatgcccga catcatcgca atgtggagcg catcgaccta tttcgcgaag gggatcgtgg
     1441 tgatcctcgt tttcatgtcg gtctggtcgg tgtcgacgtt catcatgaag tggtggcagc
     1501 tgcgccggtc cacgcgggag accgtccggt tcgttcccga gttctcgcgc ttcctgcagg
     1561 aagagcatct cgacgccgcc atcgcgctcg ccgagaagac caagaagagc cacgtggcgc
     1621 gtgtgctggg tgaggcgctc gccgaggtaa agccgctgct gcgcgaccgt gccacgatca
     1681 cggcggcgga catcaactcg gccgagcggg cggtggagcg gcagatgctg atcatcgtct
     1741 ccgagctgaa gcgcggcctc ggcattctcg cgacgatcgg cgcgacggcg ccgttcgtgg
     1801 gattgctggg cacgacgatg ggcatcgtga acgcgttcac cggcatgatg gcttcgggcg
     1861 gcggcggctc gctgggcgcc gtggccggcg gtatttccga ggcgctgatc acgacggcca
     1921 tcggcctcgt ggcggcgatt ccggcggtgt ggttctacaa ctacttcacc accaagatcg
     1981 acttcatctc cgtggagatg acgtacacgt cgaaggagct gatcgactat ctgatccgca
     2041 gtgtcgggtc ggagttcggc cggtcgatct tcaccaaaga attccaggcc cagaaggcga
     2101 cgcagtccag tggcccgatc agccgctagc gccaaggcgc cggtcaacgc caacatcaac
     2161 gtcacgccga tgatcgacgt gatgttggtg ttgctgatca t
//
LOCUS       DBNGZW010000188         3350 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_903069, whole genome shotgun sequence.
ACCESSION   DBNGZW010000188 DBNGZW010000000
VERSION     DBNGZW010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_903069"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="AC16JC_04595"
     CDS             complement(<1..258)
                     /locus_tag="AC16JC_04595"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR5164010.1"
                     /translation="MPLPMVDPTSTATALHKPSRLGRRSPNASGAMDMGRRIYISRPM
                     TRRLRRLAWAAAVATYLLIVLGAVVRITGSGLGCGEDWPVCQ"
     gene            158..1510
                     /locus_tag="AC16JC_04600"
     CDS             158..1510
                     /locus_tag="AC16JC_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5164011.1"
                     /translation="MSIAPDAFGERLPRRLGLWSAVAVLVGSTIGSGIFRTPAIIAER
                     VPAPLPMLGVWVLGGALALCGALTYAELAAMYPRSGGVYVYIREGFGRLPAFLFGWTE
                     LVLIRASALGAISTPFAEYLLRSLGFDPRLPQYAGTVHYIAAGAILVTATLNYVGVRW
                     SALILNLTTAAKFGALALLVVLAFLVGGGAFGHFAAAGGSLQPGLFGLALVSVLWAYD
                     GWGDLSFVGGEVRDPQRNLPRALVIGTCAVIGIYLLVNAAYLYLLPIDRMAQSPLVAA
                     DAAQLIIGRAGVGLVAVVVMIATFSTLLGSILTSPRIFFAMADDGLFFRAIARVHPRY
                     QTPSTAILLTAGLGVVFVLARTFEQLADQFVVAIFPFYALAAAAVFVLRRKQPERPRP
                     VRVWGYPVVPVLFVVAALIILGNALLEHPGPTGLAFIGILLGIPVYLIWLRRSRRTPA
                     "
     gene            complement(1566..>3350)
                     /locus_tag="AC16JC_04605"
     CDS             complement(1566..>3350)
                     /locus_tag="AC16JC_04605"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR5164012.1"
                     /translation="FQDNFTVYMQKHSLTFGASVEKYHSNNVFFPGAQSVYVYNSLAD
                     WYTDANDYLANPTRTVSPVTLRRFQVRYSNIPGQTEPVQPLDVLYYGVYAQDEWRPIP
                     NLTLTGGLRIDAPKFKNTAYDNAVADTMSFRDAGGATVHYNSGALPGVNLLFSPRLGF
                     NYDVHGMHQTQIRGGTGIFTGKPAYVWISNQVGNTGVLTGFIQQDNTTAFPFNPDPDH
                     YKPATVTGAPAASFQLALTDPDFKFPQVWRSNLAVDQQLPLGMTGTAELLYTKDINGI
                     AYINANLPAAQSAFTGVDARPRWTSNRIYSNVSDATVLTNQAVGHSYNVSFSVERSFN
                     SGLFTKLGFSHGVSQNTVDAGSIASGSWTGNAIALDPNNPALGFSQFSPANRFFAAVT
                     YSRDFFGIGPTSISVYFDTRNAGNASYVFAGDMNGDGVANNDLIYIPRTTSEMNFLPL
                     TVGSTTYSPVQQAVAWEAYINQDPYLSKHRGQYAERNGVFLPLVSRADLSFSQDVGRQ
                     IAGQANRLQFRLDILNVTNLIDRDWGVSQGLVTTRPLVSAGADATGAPQYRLATVGGQ
                     LISKSFTKNVSTLDVWRLQLGVRYLFNW"
BASE COUNT          474 a         1078 c         1175 g          623 t
ORIGIN      
        1 ttggcacacg ggccagtcct cgccgcaccc cagccccgag cccgtgatgc gcacgaccgc
       61 gccgagcacg atgagcaggt aggtggcgac ggcggcggcc caggcgagtc tgcggaggcg
      121 tcgtgtcata ggacgggaaa tgtatattct ccgacccatg tccatcgcgc ccgacgcatt
      181 cggtgagcgc ctgccaaggc ggctcggctt gtggagcgcc gtggcggtgc tcgtcgggtc
      241 gaccatcggg agcggcatct ttcgcacacc ggcgatcatc gccgaacggg tccccgcgcc
      301 gctgccgatg ctgggcgtct gggtgctggg cggcgcgctg gccctgtgcg gtgcgctcac
      361 ctacgccgag ctggcggcga tgtatccgcg ctcgggcggg gtgtacgtct acatcaggga
      421 aggcttcggt cgcctgccgg cgttcctgtt cgggtggact gagctcgtcc tgatccgtgc
      481 gtccgccctg ggcgccatct ctacgccgtt cgccgagtac ctgttgcgct ccctgggctt
      541 cgatcccagg ctgccccagt atgcgggcac ggtgcactac atcgcggccg gcgcgatcct
      601 cgtgaccgcg accctgaact acgtgggtgt gcgatggagt gcgctgatcc tgaacctgac
      661 cacggccgcg aaattcggcg cgctcgcgct gctcgttgtg ctggcgttcc tggtcggggg
      721 aggcgcattc ggacatttcg cggcggccgg cggctccctg caaccgggac tgttcggcct
      781 ggcgctggtc tcggtgctgt gggcctatga cggttggggc gacctgtcgt tcgtgggcgg
      841 agaagtccgg gatccgcagc gcaatttgcc gcgcgcgctc gtcatcggca cctgcgcggt
      901 gatcggcatc tacctgctgg tcaacgccgc ctacctctac ctgctgccga tcgaccgcat
      961 ggcgcaatcg ccgctggtgg cggcggatgc ggcccagctg atcatcggcc gggcgggcgt
     1021 gggactggtg gcggtcgtgg tgatgatcgc gacgttcagc acgctgctcg gctcgatcct
     1081 cacgtcgccg cggatcttct tcgccatggc cgacgacggc ctgttcttcc gggccatcgc
     1141 ccgggtgcac ccccgctacc agacaccttc gaccgcgatc ctgctcaccg cggggctcgg
     1201 cgtggtgttc gtgctggccc gtacgttcga acagctggcc gatcagttcg tcgtggcgat
     1261 cttccccttc tatgccctcg ccgcggccgc cgtgttcgtg ctgcgccgga agcagcccga
     1321 gcgcccgcgc ccggtgcgcg tgtgggggta tccggtggtc ccggtgctgt tcgtggtggc
     1381 ggcgctcatc atcctcggca acgcgctgct ggagcatccc ggtccgaccg ggctcgcgtt
     1441 catcggcatc ctgctcggca ttccggtcta cctgatctgg ctgcggcgga gtcgccgtac
     1501 cccggcctga aaacgaaacc gccccccgtt cggggggcgg tccgcgccgt tgcgcgacgt
     1561 cgcggctacc agttgaacag gtagcgcacg cccagctgca ggcgccagac gtcgagcgtg
     1621 gacacgttct tcgtgaacga cttggaaatc agctgcccac ccacggtcgc cagccggtac
     1681 tgcggggctc cggtggcgtc ggccccggcg gagaccagcg gccgggtcgt gacgagcccc
     1741 tgggagacgc cccaatcccg gtcaatcagg ttggtcacgt tcaggatgtc caggcggaac
     1801 tgcagccggt tggcctggcc cgcgatctgg cgcccgacgt cctgtgagaa gctcaggtcg
     1861 gcccgggaca ccagcggcag gaagacgccg ttccgctcgg cgtactggcc gcggtgcttg
     1921 ctcagatacg gatcctggtt gatgtacgcc tcccacgcga cggcctgctg cacgggcgag
     1981 taggtggtgc tcccaaccgt cagcgggagg aagttcatct cgctcgtggt gcgcgggata
     2041 tagatgaggt cgttgttggc tactccatcg ccgttcatgt ccccggcgaa gacatagctc
     2101 gcgttccccg cgttgcgcgt gtcgaagtac acgctgatgc tggtggggcc gatgccgaag
     2161 aagtcacggc tgtaggtcac cgccgcgaag aaccggttgg ccggggagaa ctgggagaac
     2221 ccgagcgccg ggttgttggg atcgagggcg atggcgtttc ccgtccacga ccccgacgcg
     2281 atggagcccg cgtcgaccgt gttctggctg acgccgtgcg agaagcccag cttcgtgaac
     2341 aggccgctgt tgaacgaccg ctccaccgag aacgagacgt tgtacgagtg gcccaccgcc
     2401 tggttcgtga gcacggtcgc gtcgctgacg ttcgagtaga tgcggttcga cgtccagcgc
     2461 ggccgcgcat cgacgccggt gaacgccgac tgggccgcgg ggaggttggc attgatgtac
     2521 gcgatgccgt tgatgtcctt cgtatacagg agctcggcgg tgcccgtcat gcccagggga
     2581 agctgctgat ccaccgcgag gttgctgcgc cacacctgcg ggaacttgaa gtccgggtcg
     2641 gtgagagcga gctggaagct cgccgccggc gcgcccgtga ccgtcgccgg cttgtagtga
     2701 tcgggatccg ggttgaacgg gaacgcggtc gtgttgtcct gctggatgaa gcccgtgagc
     2761 acgccggtgt tgccgacctg gttcgagatc cacacgtaag ccggcttccc ggtgaagata
     2821 cccgtgccgc cgcggatctg cgtctggtgc atcccgtgga catcgtaatt gaagccgagc
     2881 cgcggcgaaa acagcaggtt gacccccggc agggccccgc tgttgtagtg caccgtcgcg
     2941 ccgccggcat cgcggaacga catcgtgtcg gccaccgcgt tgtcgtaggc cgtgttcttg
     3001 aacttggggg cgtcgatccg caacccgccg gtcagggtca ggttcggaat cgggcgccac
     3061 tcgtcctgtg cgtagacgcc gtagtacagc acgtcgagcg gttgcacagg ctccgtctgg
     3121 ccggggatgt tcgaatagcg gacctggaac cggcgcagtg tgaccggcga gaccgtgcgg
     3181 gtcggattcg ccagatagtc gttggcgtcg gtgtaccagt cggccagcga gttgtacacg
     3241 tagacgctct gcgcgcccgg gaagaagacg ttgttggagt ggtacttctc cacgctcgcc
     3301 ccgaacgtga gcgagtgctt ctgcatgtag acggtgaagt tgtcctggaa
//
LOCUS       DBNGZW010000189         2399 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_17315, whole genome shotgun sequence.
ACCESSION   DBNGZW010000189 DBNGZW010000000
VERSION     DBNGZW010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_17315"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="AC16JC_04610"
     CDS             <1..207
                     /locus_tag="AC16JC_04610"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014386.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HIR5164013.1"
                     /translation="LILWGWAGSQYPFLVPPDLAIEDAAAPAATLRLVLIALALGAAV
                     LVPSLVYLFRIFKGGRQPSAVGHQ"
     gene            complement(201..350)
                     /locus_tag="AC16JC_04615"
     CDS             complement(201..350)
                     /locus_tag="AC16JC_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164014.1"
                     /translation="MRLLKTLVAFVVATLVGALGNEVLGPFGLVVGFIAGGVAAWWVV
                     RRLRY"
     gene            complement(347..622)
                     /locus_tag="AC16JC_04620"
     CDS             complement(347..622)
                     /locus_tag="AC16JC_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014235711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HIR5164015.1"
                     /translation="MTQTGQLVVHVVPRAKATEVVGRYGDAIRIRLAAPPVDGAANAE
                     LVRFLAERLDVPRSAVSIVRGVAARRKTVRVEGVATDAAVRVLLETP"
     gene            complement(657..1244)
                     /locus_tag="AC16JC_04625"
     CDS             complement(657..1244)
                     /locus_tag="AC16JC_04625"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR5164016.1"
                     /translation="MPGTMNFNVLLLIRYAMFAAFALAVLVALGSWLIRTRRLSPFGP
                     VGRAMRQLTDPLMRPVERRVVRAGGNPVQAGWWLVIGTAILGILVISVAQWLLASWYS
                     ITGAAHAGGRSMLLLAIGVVYDILFFAILVRVVASWLGRGRFTPWLRPFYWLTDWLVE
                     PIRRILPTAGAFDFSPLVALLVLWLLKQFVFQVLR"
     gene            1338..>2399
                     /locus_tag="AC16JC_04630"
     CDS             1338..>2399
                     /locus_tag="AC16JC_04630"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5164017.1"
                     /translation="MRIRSLIVTVAVACAASCDTAREPVYIGVAGPFAQARGSSMQLA
                     ARLAAKQINDRGGIGGRQLQLVFADDSGEDGAAVRAAQSFVDRDRVVAVVGHLSSGPT
                     LTALGVYAGAPDPIPLISPSASSPDLSGISRWMFRICPSDLAHGTQLARFARQRLGAA
                     RAAVIYTNDDYGRGVRRTFASEFQRLGGTVLEQDPYLVASGSPEPYVARLRRAGGVDV
                     LVLATQRVEAESVLREVRGAGLGWAVIGGDGLVGIETDGPLADGVRVSVSYLSDRTGE
                     RNDAFVTAYARAYGQRPDHRGAGAYDVIMLLAQAIGQVGTRRDAVRDYLASVGRARPA
                     YEGVTGRIAFDSAGDVTNKS"
BASE COUNT          369 a          843 c          844 g          343 t
ORIGIN      
        1 ctgatcctgt ggggctgggc gggcagccag tatccgttcc tggttccgcc cgatctcgcg
       61 atcgaggacg ccgcggcgcc cgcggccacg ctgcgcctcg tgctgatcgc tctggcactc
      121 ggtgcggcgg tactcgtgcc gtcgctcgtg tatctgttcc ggatcttcaa gggcggccgg
      181 cagccgtcag ccgtcggcca tcagtaacgc agacgtcgca ccacccacca cgccgcgacg
      241 ccaccggcga tgaagcctac gacgaggccg aacgggccga gcacctcgtt gccgagcgcg
      301 ccgacgagcg tagcgaccac gaacgccacc agtgtcttga gcagtctcac ggcgtttcca
      361 ggaggacccg cacggcggcg tccgtcgcca cgccctccac gcggactgtc ttccgccggg
      421 cggctacgcc gcgaacgatc gacacggcgc tgcgcggcac gtcgaggcgt tcggcgagga
      481 agcgcaccag ctccgcgttg gccgctccgt ccaccggcgg cgcggcgagc cgaatcctga
      541 tcgcgtcgcc gtaccggccc acgacctcgg tggccttggc gcgcggcacc acgtgcacga
      601 cgagctgccc ggtctgcgtc atgccggcga ccgatagccg atagccgata gcgttcttat
      661 cgcagcacct ggaagacgaa ttgcttcagc agccacaaca ccagcagcgc caccagcgga
      721 ctgaagtcga aggcgccggc ggtcgggagt atccggcgga tcggctcgac gagccaatcg
      781 gtgagccagt agaagggccg cagccacggt gtgaacctgc cgcggcccag ccacgaagcc
      841 acgacccgga cgaggatcgc gaagaacagg atgtcgtaga cgacgccgat ggccaggagg
      901 agcatgctgc gccctcccgc gtgcgccgcg ccggtgatgg aataccagga cgccaggagc
      961 cattgcgcga cggagatgac gaggatgccg aggatcgccg tcccgatgac gagccaccag
     1021 ccggcctgca ccgggttgcc gccggcccgc accacccgcc gctccaccgg ccgcatcagc
     1081 ggatccgtca gctgccgcat cgcgcggccc actggtccga agggagagag ccgccgggtg
     1141 cggatcagcc agcttcccag cgccacgagc accgccaggg cgaacgccgc gaacatggcg
     1201 tagcggatca acaggaggac gttgaagttc atcgtgcccg gcactgggtc cgttatagat
     1261 agcgggggac ggggtcatgc gtcagcgtcc tcgcggctgg cgtatcccgc ccgagttgcc
     1321 cacattagcg gcccgtgatg cgcattcgct ctctcatcgt gaccgtcgcc gtagcctgtg
     1381 cggcgtcctg cgacacggcc cgcgagccgg tgtacatcgg cgtggccggg cccttcgccc
     1441 aggcgcgcgg cagctcgatg cagctcgcgg cccgccttgc ggccaagcag atcaacgacc
     1501 gcggcggcat cggtggccgg cagctccagc tcgtgttcgc cgacgacagc ggtgaggacg
     1561 gcgccgccgt gcgcgccgcc cagtcgttcg tggaccgcga ccgcgtcgtg gccgtggtcg
     1621 gccatctgtc ttcggggccc acgctcaccg ccctcggcgt atacgcgggg gcgcccgacc
     1681 cgatccccct gatcagtccg tccgcttcca gtcccgacct cagcggaatc tcacgatgga
     1741 tgttccggat ctgccccagc gatctggcgc acggcacgca gctggcgcga tttgcccgcc
     1801 agcgtctcgg tgcggcgcgg gcggcggtga tctacacgaa cgacgactac gggcggggag
     1861 tgcggcggac cttcgcgagc gagttccagc gcctgggcgg gaccgtcctc gagcaggatc
     1921 cctatctcgt cgcgagcggg tcgcccgagc cctatgtggc gcggttgcgg cgggcgggcg
     1981 gcgtggacgt gctcgtgctc gcgacccagc gggtggaggc ggagtcggtg ctgcgggaag
     2041 ttcggggcgc cgggcttggc tgggcggtga tcgggggtga cgggctcgtc ggcatcgaga
     2101 ccgacggacc cctcgccgac ggagtgcggg tctcggtgtc gtacctctcc gaccggaccg
     2161 gtgagcgcaa cgacgcgttc gtgacggcct acgcccgagc ctacgggcaa cgccccgatc
     2221 atcgcggagc aggggcgtac gacgtgatca tgctgctggc ccaggccatc ggacaggtcg
     2281 gcacgcggcg cgatgcggtg cgtgattatc tggcgagcgt cggccgcgcg cgcccggcct
     2341 acgaaggggt cacgggacgt atcgccttcg attcagcggg cgacgtaacc aacaaatcc
//
LOCUS       DBNGZW010000190         3013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1177848, whole genome shotgun sequence.
ACCESSION   DBNGZW010000190 DBNGZW010000000
VERSION     DBNGZW010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1177848"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..433)
                     /locus_tag="AC16JC_04635"
     CDS             complement(<1..433)
                     /locus_tag="AC16JC_04635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5164018.1"
                     /translation="MSDTAQLEERAVELVRRAQAGEAAAREELLTCCHGTIRRWARVL
                     TEDADEAEDVAQEVLIRLALRLDRYAERSRFTTWLYQVTRNTAFGLRRRLTRRLRLIG
                     GMTEHMQEDHSPDPSAQTEASELRRIVERLFRELPLRQREVF"
     gene            560..1936
                     /locus_tag="AC16JC_04640"
     CDS             560..1936
                     /locus_tag="AC16JC_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HIR5164019.1"
                     /translation="MAFRTIIEPFRIHATQAIRHITEAEREAALQRVGFNLFGLHGDD
                     VLIDLLTDSGTGAMSSKQWAAMMDGDESYAGSRSFYRFQDVVRDITGLPEVIPTHQGR
                     AAERILFSVVAQPGHVVPNNTHFDTTRANIEYCGAEARDLVIPEGRQPATRHPFKGNM
                     DVAALDATIREVGREHVPLVMVTVTNNSGGGQPVSLANLKAVREVCDRHRVPLYLDAC
                     RFAENAFFIKLREPEYAAWSAKRIAQEMFALCDGCTMSAKKDGLANIGGFLAMKDKDV
                     AARCRNLLILTEGFPTYGGLAGYDLEAVATGLEEVLDEDYLTYRLRSTAYLAEKAEAA
                     GVPVVQPPGGHAVYLDAKSLLPHIPPSAYPAQSLAVALYRRGGVRGVEIGSVMFGKRH
                     PDGSETPAAMELVRLAIPRRTYTQSHVDYVGEVIAEVARRKQELRGYRIVEQGPWLRH
                     FTARFEPL"
     gene            complement(1939..2196)
                     /locus_tag="AC16JC_04645"
     CDS             complement(1939..2196)
                     /locus_tag="AC16JC_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164020.1"
                     /translation="MDNLPTKTAEAPPTQVEVIPTWRELGERFRDVMKDIGAEIGKTW
                     KKEGKELEADLQSRLLPALNRAKLEIDKLIKKLEERTKKSQ"
     gene            2356..2442
                     /locus_tag="AC16JC_04650"
     tRNA            2356..2442
                     /locus_tag="AC16JC_04650"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2390..2392,aa:Ser,seq:gga)
     gene            2547..2633
                     /locus_tag="AC16JC_04655"
     tRNA            2547..2633
                     /locus_tag="AC16JC_04655"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2581..2583,aa:Ser,seq:cga)
     gene            2702..>3013
                     /locus_tag="AC16JC_04660"
     CDS             2702..>3013
                     /locus_tag="AC16JC_04660"
                     /inference="COORDINATES: protein motif:HMM:NF039927.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HIR5164021.1"
                     /translation="MNANQPDPCPSCGAPLGGRAGCQAAFDELSAASWTSPGRGAMHN
                     MLVDVYAMQHPEEYGRSAKSYIRHLSALGCLLEHPGDARLYWARPEAGAAPVPPKPAL
                     LA"
BASE COUNT          497 a          991 c         1006 g          519 t
ORIGIN      
        1 agaagacctc ccgctgacgc agcggcaact cccggaacag ccgctccacg attcgccgca
       61 gctccgatgc ttcggtctgg gccgagggat ctggactgtg atcttcctgc atgtgctccg
      121 tcattccccc gatgagcctg agccgccggg tcaggcggcg ccgcaggccg aacgccgtgt
      181 tacgagtgac ctggtacagc caggtcgtga accgggaccg ctcggcgtag cgatcaaggc
      241 gcagcgctag ccggatcaac acctcctgcg ccacgtcctc ggcctcgtcg gcgtcctcgg
      301 tgaggacacg cgcccagcgc cggatcgtgc cgtggcagca cgtgagcagc tcttcacgcg
      361 cggccgcctc tccagcctgc gcgcgccgga cgagctcgac ggcccgctcc tccaactgcg
      421 ctgtatcgct caaccgcgct ccgaagtggc tgccctgaga cgctctcact gtgcgacgcg
      481 tttaccgggg cccggcacga gagataggtt actacggcca cgctggcccg cccatttccc
      541 ctcatgtccg agtgtcgcca tggcgtttcg caccatcatc gagccgttcc ggattcacgc
      601 gacgcaggcc attcgtcaca tcaccgaagc cgagcgggag gcggcgctgc aacgcgtcgg
      661 cttcaatctg ttcgggctgc acggcgacga cgtgctgatc gacctgctca ccgattcggg
      721 caccggcgcg atgtcgtcga aacagtgggc ggcgatgatg gacggtgacg aatcctatgc
      781 ggggagtcgc agcttctatc ggttccagga tgtcgtccgt gacattaccg ggctaccgga
      841 agtgattcct acgcatcagg gccgggcggc ggagcggatt ctgttctccg tggtggcgca
      901 gccgggacac gtcgtgccca ataacacgca tttcgatacc acccgcgcca acatcgagta
      961 ctgcggcgcc gaggcgcggg atctcgtgat tccggaaggg cggcagccgg ccacgcggca
     1021 tccgttcaag ggcaacatgg acgtcgccgc gctcgacgcc acgatccgtg aagtcgggcg
     1081 cgagcacgtg ccgctcgtca tggtgaccgt caccaacaac tccggcggtg gacagccggt
     1141 gagcctcgcc aatctgaaag ccgtgcgcga ggtgtgcgat cgccaccggg tcccgctgta
     1201 cctggacgcg tgccgcttcg ccgagaacgc gttcttcatc aagctgcgag agccggagta
     1261 tgccgcgtgg tcggccaagc ggatcgccca ggagatgttc gccctgtgcg acggctgcac
     1321 catgtcggcc aagaaggacg gactcgccaa catcgggggc ttcctcgcga tgaaggacaa
     1381 ggacgtggcg gcgcgctgcc gcaacctgct gatcctcacc gaggggttcc cgacctacgg
     1441 cgggctcgcc ggctacgatc tcgaggccgt ggcgacaggg ctcgaggaag tcctcgacga
     1501 ggactatctg acttaccggt tgcgctccac ggcgtacctg gctgagaaag cggaagcggc
     1561 cggcgtgccg gtggtacagc cgcccggcgg gcacgcggtc tacctggacg ccaagtcgct
     1621 gctgccgcac atccctccgt ccgcctatcc ggctcagtcg ctcgccgtcg ccctgtaccg
     1681 gcggggcggg gtgcgcgggg tcgagatcgg ctcggtgatg ttcggcaagc gccatcccga
     1741 cggcagcgag acgccggcgg cgatggaact ggtgcggctc gcgatcccgc ggcgcaccta
     1801 cacccagagt cacgtggact acgtgggtga ggtgatcgcg gaggtcgcgc gccgcaagca
     1861 ggagctgcgc ggatatcgaa tcgtggaaca ggggccctgg ctgcggcatt tcaccgcacg
     1921 cttcgagccc ctgtagagtt actgggactt cttcgtccgc tcctcgagct tcttgatcag
     1981 cttgtcgatc tcgagcttgg cgcggttcag ggccgggagc aaccgcgact gcaggtcggc
     2041 ctccagctcc ttgccttcct tcttccaggt cttcccgatc tccgcgccga tgtccttcat
     2101 gacgtcgcga aaccgctcgc ccagctcgcg ccaggtggga atcacctcga cctgggtcgg
     2161 gggcgcttcc gctgtcttgg tgggcagatt gtccatgggc cacctcctcg tgagagacac
     2221 attgctgccg gctcgctgaa accaccgtga ggcgcgcctc agagaggctt cagatggtac
     2281 gccggagggg ggacgaaggg ttcaggggcc ggccgcggtg gcgaaaccct tgtggcccgt
     2341 ttatttaaag ggccgggaca ggtggccgag cggctgaagg cacacgcctg gagagcgtgt
     2401 aaacgggtaa ccgtttcgtg ggttcgaatc ccaccctgtc cgtctgaata ctaggcgtgc
     2461 aggcgtgcag gtgtaaaggc gtacagcgat gctcgatgtg cactcctgaa cgccttaggt
     2521 ctgaacgcct gaacgcctag tcgtcaggac aggtgcgtga gcggttgaaa cggccggtct
     2581 cgaaaaccgg tgtgcccgta agggtaccga gggttcgaat ccctccctgt ccgttaaact
     2641 aggcgcgtag gcgcttagat gcccagtcgc ttagaaaaac ccgccgcatg aactccttgc
     2701 gatgaacgcg aatcagccgg acccgtgccc ctcgtgcggc gctccgctcg gcgggcgtgc
     2761 cggctgccag gcggcgttcg acgaattgag cgcggcgtcg tggaccagcc cggggcgcgg
     2821 ggccatgcac aacatgctgg tggacgtgta cgccatgcag cacccggagg agtacgggcg
     2881 ctccgcgaag tcctacattc ggcatctcag cgcgctgggg tgtctgctcg agcacccggg
     2941 cgacgcgcga ctgtactggg ccaggccgga ggcaggagcg gcacccgtcc ctcccaagcc
     3001 cgcgttgctc gcg
//
LOCUS       DBNGZW010000191         4438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1178024, whole genome shotgun sequence.
ACCESSION   DBNGZW010000191 DBNGZW010000000
VERSION     DBNGZW010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1178024"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="AC16JC_04665"
     CDS             complement(<1..446)
                     /locus_tag="AC16JC_04665"
                     /inference="COORDINATES: protein motif:HMM:NF040248.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5164022.1"
                     /translation="MQRMLARRALAGIAVVAGVVTLTFLLLHAAPGDPTERLIGPTAT
                     PAQVTAGRHALGLDRPLAAQYGAWLARFVRGDWGTSFATGRPVRSMIGAAWPATVRLV
                     VLSLLLSYGLGLVVGAVQAARHRRRLDTALSVASVTLVALPGYWLG"
     gene            504..1946
                     /locus_tag="AC16JC_04670"
     CDS             504..1946
                     /locus_tag="AC16JC_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5164023.1"
                     /translation="MMRTLCWLVVVGTGACGGAAGAAARRGATVLYAAGADLVSINPL
                     LTDHPLAKQVQRYVLLTTLVRYDSTLTPQPYLARHWDWSADRTTLTFRLFAGLPWDDG
                     GLTTARDVAWTLEAARDPATGYPRLTDLAAVTDVRAPDDTTVIVRFRTPQRGMPDVFT
                     DLAILPAHLLAHVPHAQLRQAAWNERPVGNGPFRFVLHEPHRRWVFTRNAAFPAALGG
                     PPRLERFIVVVVDEPMTKLAALTAGELDFAGINPAHAEFVRRNPDLTVLSYPQIFVYG
                     LVFNTRRPPFDDETIRRAAALAIDRNEIVQGFLFGFGEPAAGPVPPFVPGYVPVPPVP
                     FAPDSARRLLSGRHPEVVLLTVGSGEAALEQLLQARLAAVGFDVSIRQLELSAFHARV
                     YGPRHDFTAAVMGIPGDAGLGYLAVIGDISGVSAPADPVAAQRTLVARQAAAWLYVAR
                     GGAGMNRRVRSVTMDMRGDLVTVARWWTAP"
     gene            1943..2956
                     /locus_tag="AC16JC_04675"
     CDS             1943..2956
                     /locus_tag="AC16JC_04675"
                     /inference="COORDINATES: protein motif:HMM:NF012509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GHMP family kinase ATP-binding protein"
                     /protein_id="HIR5164024.1"
                     /translation="MRHRDLPTAFRATAPVRLDFAGGWTDVPPFSSRTGGVVVNAALA
                     LQVTAELQLGGSLVRLVSHDLAETVECTDSAGLVLDGRLNLLKAALRMFPVLGALTLE
                     TRSEVPPGSGLGSSGALDVALVACLVAARSERIDPRELAEQAWRLEAHEAGIPGGRQD
                     QLAAALGGFHRLSFRDPDVGIEPITLDPAFAAALERQLVLCYTGRSRLSGDTIARVMS
                     AYERGDRAVTGALEAMKDIALAMAEALREADLARVGTLLSQNWTRQQELDPGMRTDDM
                     ARLEGVMRDAGVLGGKAAGAGAGGCMFFLAPSGRAQPFEAAREAGATLLPVRWAWEGG
                     RAL"
     gene            2953..>4438
                     /locus_tag="AC16JC_04680"
     CDS             2953..>4438
                     /locus_tag="AC16JC_04680"
                     /inference="COORDINATES: protein motif:HMM:NF017263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III domain-containing protein"
                     /protein_id="HIR5164025.1"
                     /translation="MTAEQLATRRDAIVSSPDLAGLADHLGRRNAPVLERMPPVPEVK
                     ALLSVDGGRCPADGTALRFDPWNPDEHVCPACGRKQAGPRHHRTWARLQHLWLAERAV
                     ELAALAALTEHQPDAAARRAADLLRAYGERYFRYPNRDNVLGPSRLFFSTYLESIWIL
                     NYLSAALLLREAGRLDEATERSVHEVAEEAANLIGDFDEGFSNRQTWNDAALCAIAVW
                     FEDEALAQRAVQGETGLIAHLRGYRGDGMWYEGENYHLFALRGMLTGVGWAALAGVEF
                     WGEPELARALATALRAPALTALPDFTFPARKDARFGVSLAQPMYMELWETGLGRLKSQ
                     EQGAESTEQGADGSADQLVAWLHALYSSGPVRQELFESYLHDAPLQLLPAPCSRSQLS
                     WWSLLEMAPELPAASEAWAPPSVFLEGQGLAVLRTGGRYVSLECGASGGGHGHPDRLN
                     LTVHADGIHWLPDFGTGSYVTRDLFWYRSTLAHNAPRLDGVSQPP"
BASE COUNT          583 a         1555 c         1612 g          688 t
ORIGIN      
        1 aacccgagcc agtagccggg cagggcgacc agcgtaacgc tcgcgaccga aagggcggtg
       61 tccagtcggc ggcgatgccg cgccgcctgg acggcgccga ccacgagtcc cagcccgtag
      121 ctcagcagca aagacaggac caccagccgg accgtcgcgg gccaggccgc cccgatcatc
      181 gagcgcaccg gccggccggt ggcgaagctc gtcccccagt cgccgcggac gaagcgggcg
      241 agccaggcgc cgtactgcgc ggcgagcgga cgatcgagcc ccagggcgtg gcgccctgcg
      301 gtgacctgtg cgggagtggc ggtggggccg atcaggcgct cggtggggtc gccgggcgcg
      361 gcgtgcagca gcaggaaggt gagcgtgacg acccccgcca ccacggcgat cccggccagc
      421 gcgcggcggg cgagcatgcg ctgcatgcag caatctcgcg cggtcgggcg ggcgccgcta
      481 gaatcctgcg cgtgacgcgg cggatgatgc gaacgctatg ctggctcgtc gtcgtgggga
      541 cgggggcgtg cgggggcgcc gccggcgccg cggcgcgccg tggggctacc gtgctgtacg
      601 ccgccggcgc cgacctggtg agcatcaatc cgctgctcac ggaccatccg ctcgccaagc
      661 aggtacagcg ctacgtcctg ctcacgacgc tcgtccgcta cgattcgacg ctcacgccgc
      721 agccctacct cgcgcgacac tgggattggt cggccgatcg cacgaccctg accttcaggc
      781 tgttcgcggg gctcccctgg gacgacggtg gcctcacgac ggcgcgcgac gtcgcctgga
      841 cgctcgaggc ggcgcgcgac ccggcgaccg gctatccgcg cctcacggac ctcgcggccg
      901 tgacggatgt gcgcgcaccc gatgacacta ccgtcatcgt ccgctttcgc actccgcagc
      961 gggggatgcc cgacgtcttt accgatctgg cgattctgcc cgcccatctg ctcgcgcacg
     1021 tgcctcacgc gcagctgcgc caggcggcct ggaacgagcg cccggtggga aacgggccgt
     1081 ttcgcttcgt gctccacgag ccgcaccgcc gctgggtgtt cacgcgcaac gccgcgttcc
     1141 ccgccgcgct cggcggcccg ccccggctcg agcgtttcat cgtggtggtg gtggacgagc
     1201 cgatgaccaa gctcgccgcc ctgacggcgg gggagctcga tttcgccggc atcaacccgg
     1261 ctcacgcgga attcgtgcgt cgcaatcccg atctgaccgt gctgagctat ccgcagatct
     1321 tcgtgtatgg cctggtcttc aacacccggc gtcctccgtt cgacgacgag accattcgcc
     1381 gcgccgccgc cctcgccatc gaccgcaacg agatcgtgca gggattcctg ttcggattcg
     1441 gcgagcctgc cgccggcccg gtccccccgt tcgtgccggg ctacgtcccg gtgccgcccg
     1501 tgccgttcgc gcccgattcc gcccgccgcc tgctctcggg acgccatccg gaggtcgtgc
     1561 tgctgaccgt cggcagcggc gaggcggcgc tcgagcagct gctgcaggca cggctcgccg
     1621 cggtcgggtt cgacgtgtcc atccgccagc tcgagctgtc ggcctttcac gcgcgcgtgt
     1681 acggcccgcg gcacgatttc accgcggccg tgatggggat cccgggcgat gcggggctcg
     1741 gctacctcgc cgtgatcggc gacatttccg gggtctcggc accagcggat cccgtcgccg
     1801 cccagcgcac gctcgtcgcg cgtcaagccg cggcctggct ctacgtcgca cggggagggg
     1861 cagggatgaa tcgtcgcgtg cgcagcgtca cgatggacat gcgtggcgac ctggtgaccg
     1921 tggcccgctg gtggacggcg ccgtgagaca ccgcgatctc cccacggcgt tccgtgccac
     1981 cgctccggtg cgcctcgact tcgcaggcgg ctggaccgac gtgccgccat tttcctcccg
     2041 caccggaggc gtcgtggtca acgcggcgct cgccctccag gtcacggccg agctgcagct
     2101 cggcgggtcg ctcgtacggc tcgtgtcgca cgatctcgcc gagacggtgg aatgcaccga
     2161 ttcggccgga ctggtgctgg acggccggtt gaatctcctc aaggcggcgt tgcgcatgtt
     2221 cccggtgctg ggagcgctga cgctcgagac gcgctcggaa gtcccccccg gttccgggct
     2281 gggcagctcc ggcgcgctcg acgtggcgct cgtggcgtgt ctcgtcgccg cgcgctccga
     2341 gcggatcgat ccccgcgagc tggccgagca ggcttggcgg ctggaggcgc acgaggcggg
     2401 tatcccgggc ggacggcagg accagctggc ggccgcgctg ggcgggtttc accggttgag
     2461 ttttcgcgac cccgacgtcg gcatcgagcc gattacgctc gacccggcgt tcgccgccgc
     2521 cttggagcgg cagctggtat tgtgctacac cggccgctcg cgactctcgg gtgatactat
     2581 cgcgcgcgtg atgagcgcct acgaacgtgg cgaccgggcg gtcaccggcg cgctcgaggc
     2641 catgaaggac atcgccctgg cgatggcgga ggcgctgcgg gaggccgacc ttgccagggt
     2701 agggacgctg ctctcgcaga actggacacg acaacaggag ctggatccgg ggatgcgcac
     2761 cgacgacatg gcacgcctcg agggcgtcat gcgcgacgcg ggggtgctgg ggggcaaggc
     2821 ggcgggcgcg ggcgccggtg gctgcatgtt ctttctggcg ccgagtgggc gggcgcagcc
     2881 gttcgaggcg gcgcgggaag cgggcgcgac gctcctcccg gtgcggtggg cctgggaagg
     2941 aggacgcgca ttgtgacggc ggagcagctc gcgacgcgcc gtgacgcgat cgtgtcgtcc
     3001 cccgatctcg ccggactggc cgaccacctc ggccggcgca acgcgccggt gctcgagcgg
     3061 atgccgcccg tcccggaggt gaaagcgttg ctctcggtgg acggagggcg ctgcccggcc
     3121 gatgggaccg cgctgcgctt cgatccctgg aatcccgacg agcacgtctg tccggcgtgc
     3181 ggccggaaac aggcgggccc gcgccatcat cggacctggg ccaggttgca gcatctctgg
     3241 ctcgccgagc gtgcggtcga gctcgccgcg ctcgcggcac tcacggagca tcagccggat
     3301 gccgcggcgc gacgggccgc cgacctgctc cgcgcctacg gtgagcgcta cttccgctat
     3361 ccgaatcgcg acaacgtgct gggcccgtcc cggctgttct tctccacgta cctcgagtct
     3421 atctggatcc tgaactacct ctctgccgcc ctgctgctgc gcgaggcggg tcggctcgat
     3481 gaggcgacgg agcgatcggt gcacgaggta gcggaggagg ccgccaacct gatcggtgat
     3541 ttcgacgagg ggttctccaa tcggcaaacc tggaacgatg ccgctctgtg cgcgatcgcc
     3601 gtgtggttcg aggacgaggc cctggcgcag cgcgccgtcc agggggagac cgggctcatt
     3661 gcccacctgc gcgggtaccg cggcgacggg atgtggtacg agggcgagaa ctaccacctg
     3721 ttcgcgctgc ggggcatgct cacgggcgtg ggctgggcgg cgctcgccgg cgtggaattc
     3781 tggggtgagc cggagctcgc ccgggcgctc gccacggcgc tgcgggcgcc agcgctcacg
     3841 gcgctgcccg acttcacctt tcccgcgcgc aaggacgcgc gcttcggcgt gtccctggcg
     3901 caaccgatgt acatggagct gtgggagact ggcctgggac ggctgaagag ccaggagcag
     3961 ggagcagaga gcacggagca gggagccgac ggttccgcgg atcaactcgt tgcgtggttg
     4021 catgcgctgt attcgagcgg gccggtccgg caggagttgt tcgagtcgta ccttcacgac
     4081 gcacccctgc aactgctccc tgctccttgc tctcgcagtc agttgtcctg gtggtccctg
     4141 ctcgagatgg cgccggagct tcccgcggcc tccgaggcat gggcgccgcc cagtgtgttc
     4201 ctcgaggggc agggcctcgc cgtgctgcgc accggcggcc ggtacgtgag cctcgaatgc
     4261 ggcgcgtcgg gcggcggcca cggccatccc gatcggctca accttaccgt gcatgcggac
     4321 ggcatccatt ggcttcccga tttcggcacc ggctcctatg tgacgcgcga cctgttctgg
     4381 tatcggtcca cgctggcgca caacgcgccg cgtctggacg gtgtctccca gccgccgg
//
LOCUS       DBNGZW010000192         2928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_421412, whole genome shotgun sequence.
ACCESSION   DBNGZW010000192 DBNGZW010000000
VERSION     DBNGZW010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_421412"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="AC16JC_04685"
     CDS             <1..470
                     /locus_tag="AC16JC_04685"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR5164026.1"
                     /translation="GMPADVAMTALRQTQAEFHTDPDRVYLTGLSMGGHGTWYLAYRN
                     PGVFAAVVPICGWVLDVPQFRGSMPVVPGDSGAPIPALARRLRQVPIWIFHGEVDRVV
                     PVTGSREPAAALKDIGADVRYTEFLGLDHDSWDATYASRAFVDWLFAQRRHSR"
     gene            504..1310
                     /locus_tag="AC16JC_04690"
     CDS             504..1310
                     /locus_tag="AC16JC_04690"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015607014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 16 protein"
                     /protein_id="HIR5164027.1"
                     /translation="MGRAQAAALLGALLACAGSAPAPSIVWQDEFDGPAGATFDHAKW
                     TADTGGNGFGNQEREFYTTRAENVSLDGNGHLVITARVDSGNTCWYGPCLYSSARLKT
                     QGVFAHGYGRFEARIKIPRGQGLWPAWWLLGDNIETVGWPGSGEIDVMENIGREPGIV
                     HGTAHGPGYSGGGGIGRADTLSHGAYADDFHVFAVAWRPREIRWYVDGRLYHRLTPAD
                     LPTGANWVFDHPFFLLLNVAVGGGWPGDPNASTTFPQQMVVDYVRVYNEP"
     gene            1324..2163
                     /locus_tag="AC16JC_04695"
     CDS             1324..2163
                     /locus_tag="AC16JC_04695"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5164028.1"
                     /translation="MKRRTFIHTLGAAAAGGYLAPARRVWAGRLDRIGLKLYAVRKAM
                     AQDPEGTLAAIRAIGYTDVELLWSFDNFGRTPAQVAATLKHEGLRAPSAHVSPLILFV
                     GWERSLETAKLLGHEYLIVPDFGTDTTHSLDDWREWADRFNTAGAAARRAGLWLAFHN
                     EPGHMKPIDGTVPYDIFVDRLDPSVVRLQLDTGNMLIGGADPMRYLEKYRDRYWTFHL
                     KDALPDRSSDTGLGHGTFDFKRFLAAVPALDRKPCYVEHENAPDEMAAARSNFEYLHQ
                     LSF"
     gene            2163..>2928
                     /locus_tag="AC16JC_04700"
     CDS             2163..>2928
                     /locus_tag="AC16JC_04700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HIR5164029.1"
                     /translation="MRLDAVGVARYLRTVALLAIAALWMTARTAAQDRPRPELLVRID
                     DIGMNHSVNLALQQLAETGIPLSASVMFACPWYQEAVDILRNNPQISVGVHLVLNSEW
                     KSYRWGPVLGKEAVPSLVDSVGYFFSSSEQFLAHQYDLGEVERELSAQVERALRSGLK
                     IDYVDYHMGTAASTPQLLTVVERIADKYGLGISRYFGEVDHTMFDTPVAEKQSAFHAY
                     LDSLTPGPLNLVVIHVAQSTPEIRALVDMNNAAQNTA"
BASE COUNT          474 a          960 c          989 g          505 t
ORIGIN      
        1 tggggatgcc ggccgatgtc gcgatgacgg cgttgcggca gacccaggcc gagttccata
       61 ccgaccccga tcgcgtgtat ctgactggtc tctcgatggg cgggcacggg acctggtacc
      121 tcgcgtaccg caacccgggc gtctttgcgg ccgtggttcc gatttgcggg tgggtgttgg
      181 atgtcccgca gttccgtggg tcgatgcccg tcgtgccggg tgacagtgga gccccgatcc
      241 ccgcgttggc gcgacggctg cgccaggtcc cgatctggat cttccacggg gaagtcgacc
      301 gtgtcgtgcc cgtgacgggg tcgcgggagc cggccgccgc gctgaaggac atcggggccg
      361 acgtacgcta caccgagttc ctcggcctgg accacgattc ctgggatgcg acgtatgcgt
      421 ccagggcgtt cgtggactgg ctgttcgcgc agcgtcgaca cagccggtag tcagcgaggc
      481 gaatggtgac ttaccttagc cccatgggtc gcgctcaagc cgccgctctg ctcggcgccc
      541 tcctcgcttg tgccgggtcc gcgccggccc cgagcatcgt ctggcaggac gagttcgacg
      601 gaccagcggg cgccacgttt gaccacgcga aatggaccgc ggataccggc gggaacggct
      661 tcggcaacca ggagcgcgag ttctacacga cccgggccga gaacgtgtcg ttggacggca
      721 acggccacct cgtgatcacg gcgcgggtgg acagcggaaa cacgtgctgg tacgggccat
      781 gtctctactc gtcggcccgg ctcaagaccc agggcgtgtt cgcgcacggc tatggccgct
      841 tcgaagcccg catcaaaatc ccacggggcc aaggcttgtg gcccgcctgg tggctgctcg
      901 gcgacaacat cgaaacagtg ggatggcccg ggagcggcga aatcgacgtc atggagaaca
      961 tcggacgcga gccgggcatc gtgcacggca ccgcgcacgg tccgggctac tccggcggcg
     1021 gtggcatcgg gcgggcggac acgctgtccc acggcgcgta cgcggacgac tttcacgtgt
     1081 tcgcggtcgc ctggcgaccg cgggagatcc ggtggtacgt tgatgggcgg ctctatcacc
     1141 gcctgacgcc cgcggatctg cctactggag ccaactgggt gttcgatcat cccttcttcc
     1201 tgttgctcaa cgtcgcggtc ggcggcggct ggcccggcga tccaaacgct tcgacgacgt
     1261 tcccgcagca gatggtcgtg gactatgtcc gcgtctacaa cgagccctaa aggacagggg
     1321 accatgaaac gacgcacgtt tatccacacc ctcggcgcgg cagcggccgg gggatacctc
     1381 gcgccggcgc gccgcgtctg ggcggggcgg ctggatcgta tcgggctcaa gctgtatgcg
     1441 gtgcgcaaag cgatggcgca ggatcccgag ggcaccctgg ccgcgatccg cgccatcggc
     1501 tacaccgacg tggagctgct ctggtcgttc gacaacttcg gccggacccc cgcgcaggtc
     1561 gccgccacgc tcaagcacga gggactccgg gcgccgtcgg ctcatgtctc gcccttgatc
     1621 ctgttcgtcg ggtgggagcg cagcctcgag acggcgaagt tgctgggcca cgagtacctg
     1681 atcgtacccg atttcggtac ggacacgacg cactcgctgg acgactggcg ggaatgggcc
     1741 gatcgcttca acaccgcggg cgccgcagcg cggcgtgccg ggctctggct ggcgttccac
     1801 aacgagccgg gccacatgaa gccgatcgac ggtacagtgc cctacgacat cttcgtggac
     1861 cgtctcgatc catccgtggt tcggctgcag ctcgacaccg ggaacatgct gatcggcggc
     1921 gccgatccga tgcgctatct ggagaagtat cgcgatcggt attggacctt tcatctgaag
     1981 gacgcgctcc cagaccgctc gagcgacacc gggctcgggc acgggacgtt cgacttcaag
     2041 cgctttctcg ccgcggtgcc ggcgctcgat cggaagccgt gttatgtgga gcatgagaat
     2101 gcgccggatg agatggccgc cgcgcgcagc aactttgaat acctgcacca gttgagcttc
     2161 tgatgcgcct ggatgcggtt ggagtcgcca ggtacctccg aacggtcgcc ctcctcgcaa
     2221 tcgccgcgct ctggatgacg gcgcgcaccg ccgcccagga ccggccgcgc cccgagctgc
     2281 tcgttcgcat cgacgacatc ggcatgaacc actccgtgaa cctcgcgctc cagcagctcg
     2341 ccgagacggg catccccctc tccgcatccg tgatgttcgc gtgtccgtgg taccaggagg
     2401 ccgtggacat cctgcgaaac aacccgcaga tctccgtcgg ggtgcacctc gtgctcaact
     2461 ccgagtggaa gtcgtaccgc tgggggccgg tcttggggaa ggaggcggtg ccatcgctcg
     2521 tggacagcgt cggttacttc ttctcatcgt cggagcagtt tctggcgcac cagtatgacc
     2581 tgggcgaggt ggagcgagag ctgtcggcgc aggtcgagcg ggcgctgcgg tccggcctga
     2641 agatcgatta cgtcgattac cacatgggca cggccgcgtc gacgccgcag ctgctgaccg
     2701 tggtggagcg catcgcggac aagtatggcc tcggcatctc gcgctatttc ggcgaggtcg
     2761 atcacacgat gttcgacacg cccgtcgcgg agaagcagtc ggcgtttcac gcctacctcg
     2821 acagcctgac tcccggaccg ctcaacctgg tcgtgatcca cgtcgcgcaa tccactccgg
     2881 agatccgcgc cctggtcgac atgaacaacg cggcgcagaa cacggcaa
//
LOCUS       DBNGZW010000193         2567 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_421421, whole genome shotgun sequence.
ACCESSION   DBNGZW010000193 DBNGZW010000000
VERSION     DBNGZW010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2567)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2567)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2567
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_421421"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..234
                     /locus_tag="AC16JC_04705"
     CDS             <1..234
                     /locus_tag="AC16JC_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164030.1"
                     /translation="GEPGPADPLPVFWEDREIGRVSTVAWLPEGATLTSPGRWIGLGL
                     LRAEAGPGTPVHAGGVAARVVALPFALPRWDPA"
     gene            231..1223
                     /locus_tag="AC16JC_04710"
     CDS             231..1223
                     /locus_tag="AC16JC_04710"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5164031.1"
                     /translation="MTVLVTGGSGLVGSHVIEALRARGIAVRALTRHPAAPAVRALGA
                     EVTTGDVTDAAAWQRVAEGGLEAIVHAAAIVQRPHTPLADYEAVNVGGTRLAVETARA
                     TRARLVQVSSVSVYGGSSDYRPQAERRTEEYPFRPLAERDFYARTKRAAEELVQAAAR
                     RGDIVATAIRPCVIYGERDRLFIHRLIRAVRARVAPQIGPGTNHLACVYAGNVAAAIV
                     ADVRRTPIDAFRAYNITADAPPALTARQFLETIADACGVRVHFLRLPMPVARVAIGLW
                     SGPALARAALSFATGENPYPIDRARAELGWTPHVTTREALARTIRWVLANEKPG"
     gene            complement(1264..1431)
                     /locus_tag="AC16JC_04715"
     CDS             complement(1264..1431)
                     /locus_tag="AC16JC_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164032.1"
                     /translation="MKRLVFALALVAAVGACKKAEQQPPAAAADSSSMQMSDSSKMMS
                     DSSKMMDTTKH"
     gene            1540..>2567
                     /locus_tag="AC16JC_04720"
     CDS             1540..>2567
                     /locus_tag="AC16JC_04720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164033.1"
                     /translation="MSDRRTFLSRLAAAAAASGLRQHRATTAAPADPTIEARPSALYQ
                     QPDGRKNLLRITVSGLAAPAARATVTDRHGALVGAAGLLPSRDGTTLAGELWVPLSEP
                     ADFRIELEVGKRSVGRRRVRLVPPRRWTLYWLSTNHTDVGYTDLQERCLEVHRRNLDA
                     ALARLPMHPAYRWSAECALQVLSYVENRDQAAGDALLQAIRDGKIGFHALFANLLTGL
                     LDHETAARMVWPAGLLARERGLSYVTAQITDVPGQSLTFPMILAASGVRYLASGPNPE
                     RALPLLPRSEAEPHGLTGDWTAYPQLYWWEGPDGSRVLHWRSYHYGDALRFGFDRGAD
                     EMGHRLSD"
BASE COUNT          347 a          927 c          923 g          370 t
ORIGIN      
        1 ggggagcccg gcccggccga tccgttaccc gtgttctggg aggaccggga gatcgggcgc
       61 gtgtccacgg tggcgtggct gccggagggc gcgaccctga cctcaccggg tcgctggatc
      121 ggtctcgggc tgttgcgcgc ggaagccggc cccgggacgc cggtgcacgc cggaggtgtg
      181 gcggcgcgcg tggtcgcgct gcccttcgca ctgccgcgct gggatccggc gtgacggttc
      241 tggtcacggg cggcagcggc ctcgtgggca gtcacgtcat cgaggcgctg cgggcgcgcg
      301 ggatcgcggt gcgggcgctg acgaggcacc ccgcggcccc ggcggtgcgc gcgctgggag
      361 cggaggtgac cacgggagac gtgaccgacg cggcggcctg gcagcgcgtc gccgagggcg
      421 gactggaggc gatcgtacac gcggcggcga tcgtgcagcg accgcacacg cccctggccg
      481 actacgaggc ggtcaacgtc ggcggcaccc gcctggccgt ggagacggcg cgcgcgacgc
      541 gggcgcggct ggtccaggtg tcgtcggtct cggtgtatgg cggctcgtcg gattaccgtc
      601 cgcaggccga gcgccgcacc gaggagtacc cgtttcgccc gctcgccgag cgggatttct
      661 acgcccgtac caagcgggcg gccgaagagc tggtgcaggc ggcggcgcga cgcggcgaca
      721 tcgtggcgac cgccatccgc ccgtgcgtca tctacggcga gcgggaccgg ctcttcatcc
      781 accgcctgat acgggcggtc cgcgcccgcg tcgcccccca gatcggtccg ggcaccaatc
      841 atctcgcctg cgtctacgcg ggcaacgtcg ccgcggcgat cgtcgcggat gtgaggcgca
      901 cgccgatcga cgcattccgc gcctacaaca tcaccgccga tgcgccgccg gcgctcacgg
      961 cgcggcagtt cctggagacg atcgcggacg cctgcggggt gcgcgtgcac ttcctgcgcc
     1021 tgccgatgcc ggtggcgcgc gtggcgatcg gactgtggag cgggcccgcg ctggcccgcg
     1081 cggcgctgtc gttcgcgacc ggagagaatc cctatccgat cgaccgcgcg cgggccgagc
     1141 ttggatggac gccccacgtg acgacgcgcg aggcgctcgc gcgcaccatc cggtgggtcc
     1201 tcgcaaacga aaagcccggg tgattcccgg gcctcgctcc actcacgacg gacagcgagt
     1261 ggcttagtgc ttggtggtgt ccatcatctt gctggagtcc gacatcattt tggagctgtc
     1321 ggacatctgc atgctgctgc tgtcggccgc ggcggccggc ggctgctgct cggccttctt
     1381 gcacgcgccg acggcagcaa ctagtgccag cgcgaagacg agacgcttca tgctgtctcc
     1441 ttaaggtggt gagcggttga cgggcccaga cgggcgcaaa aactagtggc aggggcgctg
     1501 gcgtcaaggc cgccaccgtc ctaacctcca cgcgacccca tgagcgaccg ccgaacgttt
     1561 ctgagccgtc tcgccgccgc cgcggccgcc agcgggctgc gacagcatcg cgccaccacc
     1621 gctgcgccgg ccgacccgac catcgaagcg cggcccagcg ccctctacca gcaacccgat
     1681 ggacgcaaga acctcctccg gatcacggtg tcgggactgg ccgctcccgc cgcccgcgcg
     1741 acggtcaccg accgccacgg cgcgctggtc ggcgccgccg gcttgctccc gtcgcgggat
     1801 gggacgacgc tcgccggtga gctgtgggtg ccgctctccg agcccgccga cttccggatc
     1861 gagctcgagg tcggcaagcg ctccgtcggc cggcggcggg tgcggctggt tccgccgcgc
     1921 cgctggaccc tgtactggct gtccaccaac cacaccgacg tcgggtacac cgatttgcag
     1981 gaacgctgcc tggaagtcca ccgccgcaac ctggacgcgg ccctggcgcg cctgcccatg
     2041 catcccgcct atcgctggtc cgccgaatgc gccctgcagg tactctccta cgtggagaac
     2101 cgggaccagg cggccgggga cgccctgctc caggcgatcc gcgacggcaa gatcggcttc
     2161 cacgcgctgt tcgcgaacct cctgacggga ctgctcgacc acgaaaccgc ggcacgcatg
     2221 gtctggccgg ccgggctcct ggcgcgcgag cgcggcctgt cttacgtgac ggcacagatc
     2281 accgacgtgc ccggacagtc gttgacgttc cccatgattc tggctgcgag cggagtgcgc
     2341 tacctggcga gcggtccgaa ccccgagcgc gccctgccgc tgctgccccg gtccgaggcg
     2401 gagccgcatg gactcacggg tgactggacg gcgtaccccc agctctactg gtgggaagga
     2461 ccggacggca gccgcgtgct ccactggcgc agctaccact acggcgacgc gctccgcttc
     2521 ggcttcgatc gcggcgccga cgagatggga catcgcctgt cggactg
//
LOCUS       DBNGZW010000194         2360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1109229, whole genome shotgun sequence.
ACCESSION   DBNGZW010000194 DBNGZW010000000
VERSION     DBNGZW010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1109229"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..854
                     /locus_tag="AC16JC_04725"
     CDS             <1..854
                     /locus_tag="AC16JC_04725"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 interaction domain-containing protein"
                     /protein_id="HIR5164034.1"
                     /translation="ATVLLQGESGTGKEVIARTIHLSGARAAKHFVDVNCAAIPENLL
                     ESELFGHEKGAFTGAVARKIGRFQLATGGTIFLDEIADLSLTLQAKILRAVQEREIER
                     LGGGEPIPVDVRLIAATNQDLRQAMEGGRFREDLYYRLAVMVIQLPRLADRGDDLLSL
                     VSYFAKTIGERYGKKITSISHRVLGVLRNRPWPGNVRELRNVVERMVVVARGDTLCVE
                     HLPAELRADDRVPTDRHRGLLTLAEAEARHITRVLSQTGGQIGATAEILGIHRNTLTR
                     KIREYGL"
     gene            857..2122
                     /locus_tag="AC16JC_04730"
     CDS             857..2122
                     /locus_tag="AC16JC_04730"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5164035.1"
                     /translation="MEQALRLLPALEALAPLRALVLAVSRADEQTRWASAGHYLTVSK
                     HAVEAEELREHLDGLLRQVTGYLREMFEHAVAAIDAQGRGDTAAAIAALRRGGDLESR
                     IGRLKQAELWYTAALDLAVSLSDRGPELELLRELGGLCLALGRYHEAARHCQRSLALA
                     EGEFNEGAAAAACEALGNVHRAMGETVGARAWYERGLGSNGAAGSRGGWLLLRLGELA
                     RGRGDLVAAADLLDRARARMDAGADPAGMAQTLSAQGQVHIARGNLTAAFATYREALA
                     WARRASEPANLEVDIRLRLAELASDSGRWLEAEADLRQAEQAALTNGLPNRLVEVYTL
                     LGRLSGRAGEETGFVFFEQALELCRALELDARYVADVCVDYGQFHGDLGNLDASRAYL
                     ERAAETYRAIAQAAGVERVQAEMSRLGLV"
     gene            complement(2133..>2360)
                     /locus_tag="AC16JC_04735"
     CDS             complement(2133..>2360)
                     /locus_tag="AC16JC_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007936250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5164036.1"
                     /translation="LVEALVLCLGGGAIGVVLGTLGAVLLSAVAHWNTAISLYAILVA
                     FAFSASIGLFFGIWPARRAAALDPIVALRYE"
BASE COUNT          375 a          776 c          875 g          334 t
ORIGIN      
        1 cggcgacggt gctgctccag ggtgagtccg gcacggggaa ggaagtgatc gcccggacga
       61 ttcatctgag cggtgcccgc gccgccaagc acttcgtcga cgtgaattgc gccgccatcc
      121 ccgaaaatct gctggagagc gagctcttcg ggcatgagaa gggcgctttc acgggggccg
      181 tcgcgcgcaa gatcggacgc ttccaattgg ccacgggggg gacgatcttc ctcgatgaga
      241 tcgcggatct gagcctcacg ctccaggcca agattctgcg cgccgtccag gagcgggaaa
      301 tcgagcgtct gggcggagga gagccgatcc ccgtcgacgt acggctcatc gcggccacga
      361 accaggattt gcgccaggcc atggaaggcg gccggttccg cgaggatctc tactaccggc
      421 tcgcggtgat ggtcattcag ctgccgcgcc tggcggaccg gggcgacgac ctgctgtccc
      481 tggtgtccta cttcgcgaag accatcggcg aacggtacgg gaagaagatc acgtccatct
      541 ctcaccgggt gctcggcgtg ctgcggaatc ggccctggcc gggaaacgtg cgcgagctcc
      601 gcaacgtcgt cgagcgcatg gtcgtggtgg cccggggaga cacattgtgc gtggagcatc
      661 tccccgctga gctgcgcgcg gatgaccggg tccccacgga ccggcaccgg gggctgctca
      721 cgctcgccga agcggaggcg cgacatatca cccgcgtgct gtcgcagacg ggcgggcaga
      781 tcggcgcgac cgccgagatc ctcggcatcc accgcaatac gttgacgcgc aagatcaggg
      841 agtacggatt gtgaaggtgg aacaggccct gcgcctgctc cccgccctgg aggcgctcgc
      901 gccgctccgg gcgctggtcc tggcggtatc gcgggccgac gagcagaccc ggtgggcgag
      961 cgccgggcac tatctcaccg tgagcaagca cgcggtcgag gccgaggaac tgcgcgagca
     1021 cctcgacggc ctgctgcgcc aggtcaccgg gtacctgcgg gagatgttcg aacacgccgt
     1081 cgcggcgatc gatgcgcagg ggcgtgggga cacggcggcg gccatcgccg cattgcggcg
     1141 tggcggcgat ctggagtcgc gaatcggccg tctcaaacag gcggaactgt ggtacacggc
     1201 ggccctcgat ctggccgtct cgctatcgga ccgcggtccc gagctcgagc tgctacgcga
     1261 gctgggcggg ctgtgcctcg ccctcggtcg ctaccacgaa gcggcgcgcc actgccagcg
     1321 cagcctggcg ctcgccgagg gcgagttcaa tgagggcgcg gcggccgccg catgcgaagc
     1381 gctgggcaac gtccatcgcg caatgggaga gacggtgggc gcgcgggcct ggtacgagcg
     1441 gggtctgggg tccaacgggg ccgccgggag tcggggcggg tggttgctgc tgcgtctggg
     1501 cgaattggcc cggggacgcg gcgacctcgt ggcggcggcc gacctgctcg accgggcgcg
     1561 cgcacgcatg gatgcgggag cggatccggc cggcatggcg cagacgttga gcgcgcaggg
     1621 ccaggtgcac atcgcccggg gcaacctgac ggcggcgttc gccacgtatc gcgaagcgct
     1681 ggcatgggcg cgtcgtgcgt ctgagcccgc gaatctcgaa gtcgacattc ggctgcggct
     1741 ggcggagctg gcctcggatt cggggcgctg gctggaagcc gaggcggatc tgcggcaggc
     1801 ggaacaggcc gccctcacca acggcctgcc caaccgcctg gtggaggttt acacgctgct
     1861 cggccgtctg agcggccggg caggggagga aacggggttc gtgttcttcg agcaggcgct
     1921 ggagctctgt cgcgccctcg agctggatgc gcgctacgtg gccgacgtgt gcgtggacta
     1981 cggccagttc catggcgacc tcggcaacct cgacgcgtcg cgggcctacc tcgagcgggc
     2041 cgcggaaacc taccgggcga tcgcgcaggc cgccggcgtg gagcgcgtgc aggccgaaat
     2101 gtcacgactc ggcctcgtgt gagccgctcc cgctattcat agcgcagcgc gacgatcggg
     2161 tcgagcgccg ccgcccggcg ggcgggccag atgccgaaga acaggccaat gctcgcactg
     2221 aaggcgaagg cgacgagaat cgcatagagc gagatcgccg tgttccagtg cgccaccgcg
     2281 gagagcagca ccgccccgag ggtccccagc accaccccga tcgccccgcc ccccagacat
     2341 agcaccagcg cctcgaccag
//
LOCUS       DBNGZW010000195         2825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_154523, whole genome shotgun sequence.
ACCESSION   DBNGZW010000195 DBNGZW010000000
VERSION     DBNGZW010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2825
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_154523"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="AC16JC_04740"
     CDS             complement(<1..541)
                     /locus_tag="AC16JC_04740"
                     /inference="COORDINATES: protein motif:HMM:NF024792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HIR5164037.1"
                     /translation="MTVLVAVSMLALLGFLALAIDMGMLYVAHNDAQRAADAAALAGA
                     SAFMEQDAALAEPVARARAMDYALRNTFQNGPIDSAEVDIRVVPESALVGVRVQRESV
                     PTLFARVFGIRHVPIGAYAAAEAVQAGNARCLKPFAVPDMWYDENGDTNLDRVWDNGE
                     TWNFGDSPGDYYLPYSGPGG"
     gene            complement(602..1909)
                     /locus_tag="AC16JC_04745"
     CDS             complement(602..1909)
                     /locus_tag="AC16JC_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HIR5164038.1"
                     /translation="MQRNISGASGISALWRWTLAVVIVALALSAAPRAAAQRIISGAQ
                     QVVTVSAGASALLVYDDPVERFSIGETTIADAVAVSPREVLVTGKKLGTTTLLVWDKA
                     GTVKVYSVEVTADAPALERYLRTLFPGDSIGVSASSNTVTLSGHVRSAALDLQAVNIA
                     KATGAVIVDRLQAPPGRQVLLQVQFAEVNRTAAREMSSILSSVNPDRLNGVDTSMVES
                     VSDGLMRLFLGKANAQFNAVIRALRARGEFKSLAEPNLLTLPGQEASFLAGGEFPYPS
                     VQGGGQSGAITIVFKEFGVRLRFTPTLTEGGSIRLHVAPEVSSLDFANGLTLNGFQIP
                     SLLTRRAETDVELRDGQHLAIAGLLDNAWTKDVTRIPILGDIPVLGEFFKSSAAQQRR
                     TELLVVVTPVLVDATDKAPPLPTGDAGTWDWDRSLRRPGPTQH"
     gene            complement(1924..2748)
                     /gene="cpaB"
                     /locus_tag="AC16JC_04750"
     CDS             complement(1924..2748)
                     /gene="cpaB"
                     /locus_tag="AC16JC_04750"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HIR5164039.1"
                     /translation="MRQRRLWLILSLALLSGGSAAYLALAYLRDRTPRLMAAEAPRTQ
                     VAVAARDLPLGTIVQQADIHMIPWRGDAVPPGYVRDAAAIVGRGLITPVKANELLMDA
                     KLAPRGAGGGLAVTIPDGLRAVSVRVDDVIGVAGFVLPGTRVDVVLTLPPKNDRKETI
                     TQIVLQNVPVLAAGQVVQQDAQGKPITVSVLTVLVAPDDGEKLILASNEGKIQLALRN
                     SLDLAEIETPGARERGLLPGNRQGGAATGAVRAAQSDASGTVVEAYKGGVRTLIKF"
BASE COUNT          452 a         1004 c          926 g          443 t
ORIGIN      
        1 aaccgccggg accggaatag gggaggtaat agtcgcccgg cgagtcccca aagttccaag
       61 tctctccgtt gtcccacacg cgatccaggt tggtgtctcc gttctcgtcg taccacatgt
      121 ccggtaccgc gaacggcttc agacaccggg cgttcccggc ctgcaccgcc tccgccgccg
      181 cgtaggcgcc gatgggaacg tggcggatgc cgaagacccg ggcgaacagc gtgggaaccg
      241 attcgcgttg cacgcgcacg cccacgagtg cgctttcggg cacgacccgg atgtcgacct
      301 cggccgaatc gatcggccca ttctggaaag tgttccgcag ggcgtagtcc atggctcgag
      361 cccgcgcgac cggctcggcc agcgcggcgt cctgctccat gaacgcgctc gcgcccgcca
      421 aggcggcggc gtcggccgcg cgttgcgcat cgttgtgcgc gacgtagagc atccccatgt
      481 cgatcgccag ggcgaggaag cccaggagcg cgagcattga gaccgcgacc aggaccgtca
      541 cggctccacg ctccgaccgc gtaccacttg gcagcatgtg cacgaccgta tcccccatcg
      601 ctcagtgttg ggtgggccca ggccggcgga gcgaccggtc ccagtcccag gtgcccgcat
      661 caccagtggg gagcggcggc gccttgtcgg tggcgtcgac caacaccggc gtgaccacga
      721 cgagcagctc ggtgcgccgc tgttgtgcgg cgctcgactt gaagaactcg ccgagcaccg
      781 ggatgtcacc caggatcgga atccgcgtca cgtccttggt ccaggcgttg tcgagcaggc
      841 ccgcgatcgc caggtgctgg ccatcgcgca gctccacgtc cgtctccgcc cggcgcgtca
      901 acaggctcgg gatctggaag ccgttcaggg tcagcccgtt ggcgaagtcc agcgaagaga
      961 cttcgggcgc cacgtgcagg cggatgctgc cgccttcggt cagggtcggc gtgaaccgga
     1021 ggcgcacccc gaactccttg aagacgatcg tgatggcgcc cgactgcccg ccgccctgga
     1081 cgctcgggta ggggaattcg ccgccggcga ggaacgaggc ctcctgcccc ggcagggtca
     1141 gcaggttcgg ctcggcgagg ctcttgaatt ccccccgggc ccgcagcgcc cggatcaccg
     1201 cgttgaactg ggcgttcgcc ttgccaagga acagacgcat caacccgtcg gagacgctct
     1261 cgaccatcga cgtatcgacg ccgttgagcc gatcgggatt cacactcgac agaatggaag
     1321 acatttcccg cgccgccgtg cgattcacct cggcgaactg cacctggagc aggacctgcc
     1381 gccccggagg cgcctggagc cggtccacga tgacggcccc ggtcgccttg gcgatgttga
     1441 ccgcctgcag gtcgagcgcc gcgctccgca cgtggccgct caacgtgacc gtattcgagc
     1501 tcgccgacac gccgatcgaa tcgccgggga acagggtccg caggtagcgc tcgagggcgg
     1561 gcgcgtccgc ggtcacctcg acggaataga ccttgaccgt ccccgccttg tcccagacga
     1621 ggagggtcgt cgtccccagc ttcttgcccg tcaccaggac ttcccgtggc gacacggcga
     1681 ccgcgtcggc gatcgtcgtc tccccgatgg agaaccgctc caccggatca tcgtagacga
     1741 gcagcgccga tgccccggcc gagactgtga cgacctgctg tgcacccgag atgatccgct
     1801 gcgccgcggc gcgcggggcg gccgacaggg ccagcgccac gatcacgacg gcgagcgtcc
     1861 acctccacaa ggccgagata ccactggctc cgctgatatt gcgctgcacg attccgctcc
     1921 gttctagaac ttgatgaggg ttcgaacgcc gcccttgtac gcctcgacca ctgtgcccga
     1981 cgcgtcggac tgcgcggccc ggaccgcacc ggtggccgcc ccgccctgcc ggtttccggg
     2041 cagcaggccc cgctccctcg cccccggtgt ctcgatctcc gcgagatcga gcgaatttcg
     2101 gagcgcgagc tggatcttcc cttcgttcga cgccaggatc agcttctcac cgtcgtcggg
     2161 agcgacgagc acggtgagga ccgacacggt gatcggtttc ccctgggcgt cctgttgcac
     2221 cacctgtccc gccgcgagca ccgggacgtt ctggagcacg atctgcgtga tcgtctcctt
     2281 ccggtcgttc ttcggcggga gcgtcagcac cacatcgacc cgcgtgcctg ggagcacgaa
     2341 gccggcgact ccgatcacgt catcgacccg cacgctgacc gcgcgcaagc cgtcgggaat
     2401 ggtgaccgcc aggccgccgc cggcacccct aggggcgagt ttggcgtcca tcagcagctc
     2461 gttggccttc acgggcgtga tgagtccgcg gcccacgatc gcggcggcgt cgcgcacgta
     2521 cccaggcggc acggcatcac cccgccaggg aatcatgtgg atatcggcct gctgcacgat
     2581 ggtcccgagc ggcagatccc tggcggcgac ggccacctgg gtgcgcggcg cttcggccgc
     2641 catgagccgc ggcgtccggt cccgcaggta cgccagggcg agataggccg ctgagccacc
     2701 ggacagcagc gcaagcgaca ggatgagcca aagccggcgt tgtctcaacg cgacctcctc
     2761 cacgattcgt atgtggcgtt attcattgcg catcacgaac gtcgattgga gcgtgatgct
     2821 cgcct
//
LOCUS       DBNGZW010000196         2280 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1449168, whole genome shotgun sequence.
ACCESSION   DBNGZW010000196 DBNGZW010000000
VERSION     DBNGZW010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1449168"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="AC16JC_04755"
     CDS             complement(<1..352)
                     /locus_tag="AC16JC_04755"
                     /inference="COORDINATES: protein motif:HMM:NF013755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HIR5164040.1"
                     /translation="MTGYRYVDTPAALDEVIAAARAAPLAAVDTEAASFHRYVDRVYL
                     VQLSTRNATAIIDPLALRDLTSLGALLADPAVEKVFHDADYDLRILDRDYQFRARRLF
                     DTRIAAQLLGEPAIG"
     gene            complement(349..522)
                     /locus_tag="AC16JC_04760"
     CDS             complement(349..522)
                     /locus_tag="AC16JC_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164041.1"
                     /translation="MRVPTDYIRHAARAARKCAGDPLGGAPEPPDPEFYKTLAKALEE
                     IAAGLEQLGKGEE"
     gene            539..1348
                     /locus_tag="AC16JC_04765"
     CDS             539..1348
                     /locus_tag="AC16JC_04765"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5164042.1"
                     /translation="MVRPVTGIVRVTGAGARLAVRVRGEGPPLLFVHGFPLDHTMWTH
                     QLAALDGWRRIAPDLRGAGDSDVPSDDYAMGRYAEDLRAVCDGLDVAGVVCCGFSMGG
                     YVLFEFLRRHGSRVRGVVLCDTRPEPDTVEGQRARDENIALVSRGGVAALADRVVPKL
                     LGATTRAGRPELVEQVRGMALRQSAAGLVGALRAMRDRPDSTPLLSGITVPALVVHGA
                     EDEVAPPAIGRAMAARIPGAQYVEVEGAGHLAPLEQPERVTRALREFLGGL"
     gene            complement(1356..1634)
                     /gene="fdxA"
                     /locus_tag="AC16JC_04770"
     CDS             complement(1356..1634)
                     /gene="fdxA"
                     /locus_tag="AC16JC_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR5164043.1"
                     /translation="MPYIIAEPCINVKDRSCVDVCPVDCIYEAEDQLYIHPDECIDCG
                     ACEPECPVTAIFPEEDVPPQWKSYIAKNRDVFQSANPPGKPSPERGAK"
     gene            1857..2219
                     /locus_tag="AC16JC_04775"
     CDS             1857..2219
                     /locus_tag="AC16JC_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5164044.1"
                     /translation="MESGLKVFADRGFDLATLDDVAEAAGFTKGAIYRQFPSKSAFLL
                     GLFEQYAAVARAGAGARQASWFIPITLQFAAQAYRDPLLRRRLAVVLAEAPDGASPDG
                     QLLRALARVFPTTGGIVH"
BASE COUNT          320 a          709 c          857 g          394 t
ORIGIN      
        1 gcccgatcgc cggctcgccg aggagctggg cggcgatgcg ggtgtcgaac aggcgccgtg
       61 cccggaactg gtaatcgcga tccaggatac gcagatcata gtcggcgtcg tggaacacct
      121 tctccaccgc gggatcggcg agcagtgcgc ccagagaggt caggtcgcgg agggccaggg
      181 ggtcgatgat cgcggtggca ttgcgggtgg agagctggac gaggtagacg cggtcgacgt
      241 agcggtggaa gctcgccgcc tccgtgtcca cggccgcgag cggggcggcg cgggcggcgg
      301 cgatgacttc atcgagcgcg gccggcgtgt cgacgtagcg gtagccggtc actcttctcc
      361 tttaccgagc tgctccaggc cggcggcgat ctcctcgagc gccttggcga gtgtcttgta
      421 gaactccggg tccggcggct cgggagcgcc gcccagcgga tcgcccgcgc acttgcgtgc
      481 agccctggcg gcgtggcgga tataatcggt cggtacgcgc atgaggcaaa gatagcgtat
      541 ggttcgtccc gtgacgggaa tcgtgcgggt gacgggcgcc ggggcgaggc tcgccgttcg
      601 ggtgcgcggc gagggtcctc ccctgttgtt cgtgcacgga ttcccgctcg accatacgat
      661 gtggacccat cagctcgcgg cgctggacgg ttggcgccgc atcgctcccg acctgcgtgg
      721 ggccggggac tcggacgtcc cgtcggacga ttacgcgatg ggccggtatg cggaggatct
      781 ccgcgccgtg tgcgatgggc tggacgttgc cggcgtcgtg tgctgcgggt tctccatggg
      841 aggatacgtc ctcttcgaat tcctgcggcg ccacggctcg cgcgtgcggg gtgtggtgct
      901 gtgcgacacc aggcccgagc cggacacggt cgaggggcag cgagctcggg acgagaacat
      961 cgcgctcgtc tcgcgcggcg gggtcgcggc gctggccgat cgggtggtac cgaagctgtt
     1021 gggggcgacg acgcgggccg gccgtccgga gctcgtggaa caggtgcgcg gcatggcgct
     1081 gcggcagtcc gcggcaggat tggtcggagc gctgcgcgcg atgcgggacc gtccggattc
     1141 tactccgctg ctgagcggga tcaccgtacc ggcactggtc gtgcacgggg ccgaggatga
     1201 ggtggctcct ccggccatcg gccgcgcgat ggcagcgcgg atccccggtg cacagtacgt
     1261 cgaagtggag ggcgccggac acctggcgcc gctggagcag cccgagcggg tcacccgggc
     1321 gctgcgggaa ttcctcggag gattgtaggc cggggctact tcgcgccgcg ctccggcgat
     1381 ggcttgcccg gcgggttggc gctctgaaac acgtcgcggt tcttggcgat gtaggacttc
     1441 cactgcggcg ggacgtcttc ttcggggaag atcgccgtca ccggacactc cggctcgcac
     1501 gccccgcagt cgatgcactc gtcgggatgg atgtagagct ggtcctctgc ctcgtagatg
     1561 cagtccaccg gacagacatc cacacaggag cggtctttga cgttgatgca gggctcggcg
     1621 atgatatacg gcatcagcac gtctccggaa gtcgcggcgc agcggcctgg ttggccgcgt
     1681 tgagagccaa acctagaccg ggagcgagtc gcgtcaaggc gggcgctgag atactcactg
     1741 gtatccggag gctcaacctg gcggtcccgg gttgccattg tagatacccg ctagtatctt
     1801 agatacgtat gggttccgtc cgccagaggc gcaaggcaga aacccgcagt ctgctcgtgg
     1861 aatccggcct gaaggtattc gccgaccggg gcttcgatct cgccacattg gacgatgtcg
     1921 ccgaggcggc ggggttcacc aagggagcga tctaccggca gttcccctcc aagagcgcgt
     1981 ttctcctcgg tctcttcgag cagtatgcag cggtggctcg cgccggcgcg ggcgcgcggc
     2041 aggcgtcctg gttcatcccg atcacgctgc aattcgccgc ccaggcgtat cgggatccgc
     2101 tgctgcggcg ccggctcgcg gtcgtcctgg cagaggcgcc ggatggcgcc tcgccggatg
     2161 ggcagttgct gcgggccctg gcgcgcgtgt ttcccacgac gggcgggatc gtccattgag
     2221 cgcgggatac agcgcctctt cccgggccgc ccggctgtcg cccgtcgacg cgattcgtta
//
LOCUS       DBNGZW010000197         4394 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_154776, whole genome shotgun sequence.
ACCESSION   DBNGZW010000197 DBNGZW010000000
VERSION     DBNGZW010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4394
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_154776"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1843
                     /locus_tag="AC16JC_04780"
     CDS             <1..1843
                     /locus_tag="AC16JC_04780"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/OMP85 family outer membrane protein"
                     /protein_id="HIR5164045.1"
                     /translation="ARVSVEGNTHYADAEIVAHMATRPEGFWWWRKGEYDDEKLRADM
                     QERLPKFYGAHGFVDFQVLDDTLLVNDSSGKAELVLRVSEGEPYHVGTFEIVGNRRFS
                     TEDLRQLYPFKGEQRTGLLGLGGVARGPAVFSAARWDDATTGLYALYHNEGYIYTQVR
                     PDVIRRVGADRKPVVDLRWVINEGQPAIVNRVEIVGNDVTHERVIRDQIVLLPGDVFR
                     QDALLRSYQNISNLGFFNQPLPPPETPQANDQGDIDVVFHVSEKHTGNVNFGASVGQG
                     TGLGGFLGLEEPNLFGQGKRGRFQWQFGKNINDFDVSFTDPGIRESRISGTIDAHNTR
                     LRYNIADLGQVRRRGASVQFGFPAFHDRYARLFVSYGLDQQTVTGSASNVAFSQVFNC
                     RNCVRSTVGVSVLRDNRINLPFPTGGTMASVGLSHSGGVLGGDGNFQRLDLEGHWYAP
                     LGQLGKTSNPMPVVLGLSTRSGLVFGNSPFFDQLFSLGGTQYGIPLRGYDEFSITPQG
                     YDPLANTRGAAPTAFGKAYFLMTGEVGLRLSQMFYVNAFYDAGNLWARAVDWNPTRLF
                     RGAGIGLDVISPLGPLGLDWAYGFDKVDAAGRPAPGWKLHFKLGNFF"
     gene            1867..2424
                     /locus_tag="AC16JC_04785"
     CDS             1867..2424
                     /locus_tag="AC16JC_04785"
                     /inference="COORDINATES: protein motif:HMM:NF015871.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH/Skp family outer membrane protein"
                     /protein_id="HIR5164046.1"
                     /translation="MRRVVFVCLAVVGGVVPAAAQNPPAAARPGAQAPTGQRVAFINS
                     RTILDSTPGYAAAESTFYREFQGMRDEVQKLQQQLDSAVQAFDQASIALSPAAKDAKR
                     RDLQQMQDRMTRRSTSLDSLARARNQELLGPFQARINLIIQGIRAEGNYAFIFDGDAQ
                     GTGIVAADPALNLTSKVIGRLRQTP"
     gene            2439..3491
                     /gene="lpxD"
                     /locus_tag="AC16JC_04790"
     CDS             2439..3491
                     /gene="lpxD"
                     /locus_tag="AC16JC_04790"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HIR5164047.1"
                     /translation="MSAAPRRTLSAADVAALTGGRLIGSPDVTVAAIAPLERAGPGDL
                     SFLASARYVPYLHRSRAAIVLCAEAFAAEPSGPATRIIVPDPHAALVDVLGALYPPPA
                     WTPGVHATAVVGPGTTWIDPVAIGPHVVLGRGVRLGRNVRVGAQCVIGDGVTIGDDSE
                     LNPQVTCYAGTTIGRRALLHSGVRLGGDGFGFLPGRDGAPHRKIPHVGRCVIGDDVEI
                     GTNTCVDRGSVDDTVIDDGTKIDNLVHIAHNVHVGKRCLIMALAGIAGSCRIEDDVII
                     AGQVGLSDHVTIGRGARVLVQGGVIGDVPPGGTYWGTPARPHREVLRAQAALYRSRPR
                     SAGRKQPTEPGDGDAR"
     gene            3475..>4394
                     /gene="lpxC"
                     /locus_tag="AC16JC_04795"
     CDS             3475..>4394
                     /gene="lpxC"
                     /locus_tag="AC16JC_04795"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: protein motif:HMM:TIGR00325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0103117 - UDP-3-O-acyl-N-acetylglucosamine
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HIR5164048.1"
                     /translation="MATPAKRRGIAQETAVTGTGLHTGAATRATFRPGEAGRGVVFRR
                     TDLEGSPEVPARLSEVEAVERRTAIGHGAATIHTVEHLLAAVAAHEIDDLLIELTGPE
                     PPILDGSVGPYFAALAEAHPVEVDGEPTVLTVQAPFTVTDGDASYLVAPSKRLQLTVT
                     IEFPHPLIGRQVGSYDVSAENFARDLAPARTFGFTSEVSALQAKGLIKGASTASAIVL
                     DERGVVNGGQLRWPDEFVRHKAADILGDLALTGGRVRAHVVATKPSHAGNVALARALA
                     RAAKRSGVPAMDIGRIMDVLPHRYPFLLVDR"
BASE COUNT          671 a         1502 c         1553 g          668 t
ORIGIN      
        1 cgcacgcgtg agcgtcgaag gcaacacgca ctatgcggac gccgagatcg tcgcccacat
       61 ggccacccgt ccggaaggct tctggtggtg gcgcaagggg gaatacgacg acgagaagct
      121 gcgggccgac atgcaggagc ggctgcccaa gttctacggc gcgcacggct tcgtggactt
      181 tcaggtgctg gacgacacat tgctcgtgaa cgactcgagc ggcaaggccg agctcgtgct
      241 ccgggtgagc gaaggcgagc cgtatcacgt cggcacgttc gaaatcgtcg gcaaccggcg
      301 gttctccacc gaggatctgc ggcagctcta cccgttcaag ggggagcagc gtaccggcct
      361 gctggggctg ggcggggtgg cccgcggacc ggccgtgttc agcgccgccc gctgggacga
      421 cgcgacgacc gggctgtacg cgctgtatca caacgaggga tacatctaca cgcaggtgcg
      481 gcccgacgtg atccgccgcg tcggcgccga ccgcaagccg gtcgtggacc tgcgctgggt
      541 gatcaacgaa ggccagccgg ccatcgtcaa ccgggtcgag atcgtcggca acgacgtcac
      601 gcacgagcgc gtgattcggg atcagatcgt gctgctgccg ggcgacgtat tccgccagga
      661 cgcgctgttg cgctcctacc agaacatctc gaacctcgga ttcttcaatc agccgctgcc
      721 gccgccggag acgccgcagg cgaacgacca gggagatatc gacgtagtgt tccacgtgag
      781 cgagaagcac acggggaacg tcaacttcgg cgcgtccgtg gggcagggca ccgggctggg
      841 cgggttcctg ggtctggagg agcccaacct cttcgggcag ggcaagcgcg gccgtttcca
      901 gtggcagttc ggaaagaaca tcaacgactt cgacgtctcg ttcaccgatc cggggatccg
      961 cgagtcgcgc atctcgggga cgatcgacgc gcacaacacg cggttgcgct acaacatcgc
     1021 cgatctgggc caggtgcggc gccgcggggc cagtgtgcag ttcggcttcc cggccttcca
     1081 tgaccgctac gcccgcctgt ttgtctccta cggcctcgat cagcagaccg tgaccggctc
     1141 ggcgtccaac gtggccttca gccaggtgtt caactgccgc aactgcgtgc gctccacggt
     1201 gggcgtctcg gtgctgcgcg ataaccggat caacctgccg ttccccaccg gtgggaccat
     1261 ggcctcggtc ggcctgtcgc acagcggggg ggtgctgggc ggcgacggca acttccagcg
     1321 gctggacctc gaggggcatt ggtacgcgcc gctcggccag ctcgggaaga ccagcaaccc
     1381 gatgccggtg gtgctcgggt tgtcgactcg ctcgggtctc gtgttcggca actcgccgtt
     1441 cttcgatcag ctgttcagcc tgggcggcac gcaatacggc attccgctcc gcggctacga
     1501 cgagttctcg atcacgcccc agggatacga tccgctcgcg aacacgcggg gcgccgcccc
     1561 caccgccttc gggaaggctt acttcctcat gaccggggag gtgggtctgc gcctgtcgca
     1621 gatgttctat gtgaacgcgt tctacgacgc ggggaatctc tgggctcgcg ccgtcgactg
     1681 gaaccccacg cggttgttcc ggggcgcggg aatcggtctg gacgtcatct cgccgctcgg
     1741 cccgctggga ttggactggg cgtacggctt cgacaaggtc gacgcggccg gccggcccgc
     1801 cccggggtgg aaactgcact tcaaactcgg caacttcttc tgatgcccct ttcccacgga
     1861 gtggttatgc ggcgggtagt gttcgtgtgc ctggcggtcg tcggtggggt agtaccggcg
     1921 gcggctcaaa atcctcctgc ggcggcccga ccgggcgcgc aggccccgac gggacaacga
     1981 gtggccttca tcaactcccg gacgattctc gacagcacgc ccgggtacgc cgccgcggag
     2041 tcgacgttct accgcgagtt ccagggcatg cgcgacgagg tgcagaagct gcagcagcag
     2101 ctcgactcgg cggtgcaggc gttcgatcag gcctcgatcg ccctctcccc ggccgcgaag
     2161 gacgccaagc ggcgtgatct gcagcagatg caggaccgca tgacgcggcg ctccacgtcg
     2221 ctcgattcac tggcgcgcgc gcgcaaccag gagctgctcg ggccgttcca ggcgcggatc
     2281 aacctcatca tccaggggat ccgggccgaa gggaactacg ccttcatctt cgacggcgac
     2341 gcgcagggca ccggcatcgt cgcggccgac cccgcgctga acctgacgtc gaaggtcatc
     2401 ggtcggctgc ggcagactcc ctgaacggcg agggggcggt gagcgccgcg ccccgacgta
     2461 cgctgtcggc cgcggacgtc gcggcgctca cggggggccg cctcatcggc tccccggacg
     2521 tcacggtcgc cgcgatcgcg cccctggagc gggccggtcc cggcgacctc tcgtttcttg
     2581 ccagtgcccg ctatgtcccc taccttcacc ggtcgcgcgc cgccatcgtg ctctgtgccg
     2641 aagcgttcgc ggcggagccg tccggtcccg ccacccgcat catcgtaccg gatccgcatg
     2701 ccgcgttggt ggacgtgctc ggcgcgctct atccgccccc ggcctggacc cccggtgtgc
     2761 acgcgacggc ggtggtcggg ccggggacca cctggatcga tccggtggcg atcggccccc
     2821 acgtggtgct ggggcggggg gtccggctgg gacgcaacgt ccgggtcggt gcgcagtgcg
     2881 tcatcggcga cggcgtcacg atcggagacg actcggagtt gaatccgcag gtcacctgct
     2941 acgccggcac gacgatcggc cggcgggcgt tgctgcacag cggtgtgcgc ctcggcggcg
     3001 acggcttcgg cttcctgccc gggcgcgacg gggcgccgca tcgcaagatc ccccacgtgg
     3061 ggcgatgcgt gatcggcgac gacgtggaaa tcgggacgaa cacctgcgtg gaccggggca
     3121 gcgtggacga cacggtgatc gacgacggca ccaagatcga caacctcgtg cacatcgcgc
     3181 acaacgtgca cgtcggcaag cgatgcctga tcatggcgct cgccgggatc gcgggctcgt
     3241 gccgcatcga ggatgacgtc atcatcgcgg gccaggtcgg gctctccgac cacgtgacga
     3301 tcggccgggg cgcacgcgtc ctggtgcagg gcggcgtcat cggcgatgtg ccccccggcg
     3361 gcacgtattg ggggacgccg gcgcgaccgc atcgcgaggt gctgcgcgcc caggcggccc
     3421 tgtaccgctc ccgaccccgc agcgcagggc ggaaacagcc caccgagccc ggcgatggcg
     3481 acgcccgcta agcgtcgcgg catcgcccag gaaaccgcgg tcaccggcac cgggctgcac
     3541 accggcgcgg cgacgcgagc cacgttccgc ccgggcgagg ccggacgcgg tgtggtgttc
     3601 cgccgtacgg atctggaggg ctcgccggaa gtccccgccc ggttgtcgga agtcgaagct
     3661 gtcgagcggc gcacggccat cggccacggc gcggccacga tccacacggt ggagcacctg
     3721 ctcgccgcgg tggccgccca cgaaatcgac gacctcctca tcgagctgac cggtcccgag
     3781 ccgccgatcc tggatggaag cgtgggtccc tatttcgccg cgctcgccga ggcgcatccg
     3841 gtggaggtgg acggagagcc gacggtgctc accgtgcagg caccgttcac cgtgaccgat
     3901 ggcgacgcga gctatctcgt cgccccgagc aagcgcctgc agctgacggt gacgatcgag
     3961 ttcccgcacc cgctcatcgg acgccaggtg ggcagctacg acgtgtcggc ggaaaatttc
     4021 gcgcgcgatc tcgcgcccgc ccgcacgttc ggattcacga gcgaggtgtc ggccctgcag
     4081 gcgaagggcc tgatcaaggg cgcctcgacc gcgagcgcca tcgtgctgga cgagcggggc
     4141 gtggtgaacg gcgggcagct gcgctggccc gatgagttcg tgcgtcacaa ggcggccgac
     4201 atcctgggcg acctggcgct gaccggcggg cgggtacggg cccacgtcgt ggcgacgaag
     4261 cccagccacg ccggcaacgt ggcgctggcg cgggccctgg cccgcgcggc caagcgcagc
     4321 ggcgtgccgg cgatggacat cgggcgcatc atggatgtcc tgccgcaccg ctacccgttc
     4381 ctgctggtgg accg
//
LOCUS       DBNGZW010000198         3707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_972635, whole genome shotgun sequence.
ACCESSION   DBNGZW010000198 DBNGZW010000000
VERSION     DBNGZW010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3707
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_972635"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..434)
                     /locus_tag="AC16JC_04800"
     CDS             complement(<1..434)
                     /locus_tag="AC16JC_04800"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR5164049.1"
                     /translation="MDLNLLKQAAIFQDLDEGELARVAEVCREQKFTVGQYIFKEGEP
                     GNRLFIIADGEVRISRTIPGSGEEALAVLKPGANFGEMAIFDRSERSTDAIANTACTL
                     ITISRSDFELLLDFNRDIAYKVLWSCVRLLSSRLRVTNDNLRS"
     gene            470..1009
                     /locus_tag="AC16JC_04805"
     CDS             470..1009
                     /locus_tag="AC16JC_04805"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR5164050.1"
                     /translation="MKPLLGLADLLTLLRLPLAAAFPFAPRPLERLAIVALAVASDLA
                     DGLVARRTGGSRWGPWLDPVADKVFMASAFITLAGHGVLHPLEITGVLLRDIVAALSF
                     TGALILRRPVALPARAGGKAVTVCQVLTLIALIAGSPLTRSLAWATAAVSVYAIWDYG
                     RAATHRGAGDRAPADKREG"
     gene            1009..1419
                     /locus_tag="AC16JC_04810"
     CDS             1009..1419
                     /locus_tag="AC16JC_04810"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR5164051.1"
                     /translation="MAGNTANVSVRWRQQLVFEGGGAGRPPLVVDGDTQVATSPVETL
                     LLAAANCTGADVVEILVKMRVVLRTFDIDVEGTRREEYPRRFTAIHFRFRVAGEGVDE
                     AKMRRAVDLSLEKYCSVMASLASDIRVTYDVTVA"
     gene            1412..1876
                     /locus_tag="AC16JC_04815"
     CDS             1412..1876
                     /locus_tag="AC16JC_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164052.1"
                     /translation="MRSALAPLVLAALTHPLAGQQLTATELDAGLVATWARRTFSGVA
                     IGIARRPGGEGRLALAAAFGSLDGHAALRVEATAQFLVLPGAKSGISPYGGVGVAYLG
                     ARPYRGAGMLVLLVGVEAAPGRSRGWFGELGLGGGLRLRGGYRWRHLPPWWS"
     gene            complement(1928..2194)
                     /locus_tag="AC16JC_04820"
     CDS             complement(1928..2194)
                     /locus_tag="AC16JC_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164053.1"
                     /translation="MAKTREPRKTAIPTTFEQARDELFSHILRCGVLEAAPEHQKEWF
                     DDTMLYLADRYEDLTEPEIEQLRTLGERYCRPVVPRPSPVAVNG"
     gene            2291..2593
                     /locus_tag="AC16JC_04825"
     CDS             2291..2593
                     /locus_tag="AC16JC_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164054.1"
                     /translation="MHDQTYGPERARRLVALLRSVAARIQELDRDGRLLEAAPELQRL
                     LGNARSELFHYEVRVTYDTPEIAESRRIVEEAERHLGELGLDEPDDDQPWRSNDDG"
     gene            2590..3267
                     /locus_tag="AC16JC_04830"
     CDS             2590..3267
                     /locus_tag="AC16JC_04830"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR5164055.1"
                     /translation="MKRFYLLLGLVAVVGAVLIWQASRPSSGGAPASDEAAPVPVTAA
                     DSAFRGYVLGSDSAPVEIVEYADYECPVCAQFGAVQFPDIRQQLIETGRVRWRFRDFP
                     LVGVHRWSRLSALVVACASEQGKAWEAMDALFQRHAEWAQRPSDPTGLFADRVRGIGL
                     DMNGYRACMDANRYAGRIEASRQEGLARNVGGTPTFFVNGREYNYRRATSDEFLALVD
                     SLTRSRR"
     gene            3267..>3707
                     /locus_tag="AC16JC_04835"
     CDS             3267..>3707
                     /locus_tag="AC16JC_04835"
                     /inference="COORDINATES: protein motif:HMM:NF019499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HIR5164056.1"
                     /translation="MRHRQAIALLALVGFGIALYLWLYKMGYIGRLQCGTGSCEYVQT
                     SPYGDLWGVPVAFYGVLGYAAIFAVALTGLQPALLSRRWPTQLVAVLAAGGVLFSFYL
                     TYVELFVLHAICRWCLGSAVVITLIAAVAVPAALRAERGEGGGGR"
BASE COUNT          501 a         1175 c         1409 g          622 t
ORIGIN      
        1 gaccgcaggt tgtcgttcgt gacccggagg cgggaggaga gcaggcgcac gcacgaccac
       61 agcaccttgt aggcgatgtc gcgattgaag tccagcaaca gctcgaagtc cgagcgggag
      121 atcgtgatca gggtacaggc ggtgttggcg atcgcatcag tggaccgctc ggagcggtcg
      181 aagatcgcca tctccccgaa gttcgcgccg ggcttgagca cggccagcgc ctcttcgccc
      241 gagccgggga tggtccggga gatgcggacc tcgccatcgg cgatgatgaa cagccggttg
      301 cccggctcgc cttccttgaa gatgtactgg ccgacggtga acttctgctc gcgacacact
      361 tccgcgacgc gggcgagctc accctcgtcc agatcctgga agatcgcggc ctgcttcagc
      421 agattcaggt ccacgcggtg aaggtatcgg ccctgtgagg gtggcacaag tgaaaccgct
      481 cctcggattg gcggacctgc tgacgctgtt gcggctcccg ctcgcggcgg cgtttccgtt
      541 cgcgccccgg ccgctggaac ggctggcgat cgtggccctg gccgtggcga gcgacttggc
      601 cgacggcctc gtcgcccggc gcaccggcgg atcccgctgg gggccgtggc tcgacccggt
      661 cgccgacaag gtcttcatgg cgagcgcgtt catcaccttg gccgggcacg gcgtcctcca
      721 cccgctggag atcaccggcg tgctgctgcg ggacatcgtc gcggcgctct cgttcacggg
      781 cgcgctgatc ctgcgccggc cggtcgcgtt gccggcccgg gcggggggca aagccgtcac
      841 ggtctgccag gtgctcacgc tcatcgcgct gatcgcggga tcgccgttga cgcgctcgct
      901 ggcgtgggcc acggcggcgg tctcggtgta tgcgatctgg gattacgggc gcgcggcgac
      961 tcaccggggg gccggagaca gggcgccggc agacaagagg gaggggtagt ggcggggaat
     1021 accgcgaatg tgtcggtgcg ctggcgccag cagctcgtct tcgagggcgg cggggcaggc
     1081 cggccgcccc tggtggtcga tggcgacacg caggtcgcca cttcgccggt ggagacgctc
     1141 ctgctggcgg ccgcgaactg taccggcgcc gacgtggtcg agatcctggt aaagatgcgc
     1201 gtcgtgctgc gcaccttcga catcgacgtc gaggggacgc ggcgggagga gtatccccgc
     1261 cgcttcacgg cgatccactt ccgcttccgg gtagcagggg aaggcgtgga tgaggccaag
     1321 atgcgtcggg ccgtggacct gtcgctcgag aagtactgtt cggtgatggc gtcgctcgcg
     1381 tccgacatcc gcgttaccta cgacgtcacc gttgcgtagc gcgctcgcgc cgctggtgct
     1441 ggcggcgctg acgcacccgc tcgcgggtca acagctcacg gccaccgaac tggatgcggg
     1501 actggtcgcc acctgggcgc gacgcacgtt ctccggcgtc gcgatcggca tcgcgcggcg
     1561 tcccggcggg gagggccggc tggcgctggc ggcggcgttc ggctcgctgg acggccacgc
     1621 ggcgctgcgg gtcgaggcga cggcccagtt cctggtgctc ccgggcgcaa agagcggaat
     1681 ctccccctac gggggcgtcg gtgtggccta tctcggggcg cggccctacc ggggagcggg
     1741 gatgctggtg ctgctggtcg gtgtcgaagc agccccgggg cggtcgcggg ggtggttcgg
     1801 ggagctgggt ctgggtggcg gactaagact ccgtgggggg taccgctggc gccacctgcc
     1861 gccgtggtgg tcctgaacgc tacgggcgcc cggtggggcg cccggttggc cccgcgccgg
     1921 tctcggctca gccgttcact gcgacggggg agggccgggg taccaccggc cggcagtagc
     1981 gttcccccag ggtccggagc tgttcgatct ccggctcggt caggtcctca tagcgatcgg
     2041 caaggtacag catggtatcg tcgaaccact ccttctggtg ctcgggcgcg gcttccagca
     2101 cgccgcagcg aaggatgtgg gagaacaact cgtcacgagc ctgctcgaag gtggtcggga
     2161 tcgcagtctt tcggggctca cgggttttgg ccattcggag gggctcctct cgggatgtgg
     2221 gctgtcgggg gcgcaatcta atggggcccc cccttccagg caaacgggcg tggtataatc
     2281 agaggtgagc atgcacgatc agacctacgg cccggagcgc gcgcgtcgtc tcgtggcgtt
     2341 gctgcgcagc gtggcggcac ggattcagga gctcgaccgg gatggccgcc tgctcgaggc
     2401 ggcgcccgag ctgcagcgat tactgggcaa cgcgcggagc gagttgtttc attacgaagt
     2461 tcgcgtgacc tacgatacgc ccgagatcgc cgagagccgg cgcatcgtgg aggaagccga
     2521 gcggcacctc ggcgagctcg gtctggacga gccggacgac gaccaaccgt ggcggagcaa
     2581 tgacgacgga tgaagcgctt ttacctgctc ctgggtctgg tcgcggtggt gggcgctgtg
     2641 ctcatctggc aggcctcgcg gccgtccagc gggggcgccc ccgcctcgga cgaagccgcc
     2701 ccggtgcccg tcaccgcggc ggacagcgcg ttccgcgggt acgtcctcgg cagcgacagc
     2761 gcgccggtgg agatcgtgga gtacgcggat tacgagtgtc ccgtgtgcgc gcagttcggg
     2821 gccgtgcagt tcccggacat ccgtcagcag ctgatcgaga cggggcgcgt ccgctggcgc
     2881 ttccgtgact tcccgctcgt gggcgtccat cgctggagcc gcctgtcggc cctcgtggtg
     2941 gcgtgcgcga gcgagcaggg gaaggcgtgg gaagccatgg atgcgctgtt ccagcggcac
     3001 gccgagtggg cgcagcggcc ctccgatccc accggactgt tcgcggatcg ggtccgcggc
     3061 atcgggctcg acatgaacgg atatcgcgcc tgcatggacg cgaaccgcta cgcaggccgg
     3121 atcgaggcga gccgccagga aggtctggcg cgcaacgtgg gcggcacgcc gacgttcttc
     3181 gtgaacgggc gggagtacaa ttaccggcgc gccacctccg atgagttcct cgccctggtg
     3241 gacagtctca cccggtcgcg gcgctagtgc gtcaccggca ggcgatcgcc ctgctcgcgc
     3301 tggtcgggtt cggaatcgcg ttgtacctgt ggctctacaa gatgggttac atcgggcggc
     3361 tgcagtgcgg cacggggagc tgcgagtacg tccagaccag cccctacggc gacctgtggg
     3421 gcgtgccggt ggcgttctac ggggtcctgg ggtacgcggc gatcttcgcg gtggcgttga
     3481 cgggcctgca gcccgccctc ctgagccgcc gctggcccac gcagctcgtg gcggtgctcg
     3541 cggccggcgg tgtgttgttc agtttctatc tcacctacgt cgagctgttc gtgctgcacg
     3601 cgatctgccg ctggtgtttg ggcagcgcgg tagtcattac cctcatcgcg gcggtcgcgg
     3661 tgccggctgc gctacgggcg gagcggggag aggggggggg ggggagg
//
LOCUS       DBNGZW010000199         3066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1587287, whole genome shotgun sequence.
ACCESSION   DBNGZW010000199 DBNGZW010000000
VERSION     DBNGZW010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1587287"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..1457)
                     /locus_tag="AC16JC_04840"
     CDS             complement(123..1457)
                     /locus_tag="AC16JC_04840"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR5164057.1"
                     /translation="MEQERWLGELTTFLRIPSISTLPAHDADCRRAAEWVATDLRRLG
                     CRDVQLLGSETHPVVWGVGPDVPGAPTVLIYGHYDVQPPDPINEWTSPPFEPSLRDGR
                     LYARGAADDKGQVFCLLRAIETLGAPPVNFRFLIEGEEEFGSTVLFDLLRREPERTRA
                     DVALVADMSYLAPGWPAVYTSLRGMCYAELAVRTAKHDLHSGEYGGAAPNAHEELCRL
                     LVRLKGPNGRINIPGLYAAVQAPSARERAAWKRLPFREKDFLNRRVQAKALTGLKSHS
                     VLERLWALPTFEIHGITGGFTGAGAKTVIPAEAVAKVSLRLVPNQKMRTVERQLAAAV
                     KKLAPKYVTATVRFLHGGDPARVKIDHPAFALLDRAFHEVVGRGTVPARAGGSIPVVP
                     ALSREGAAVLLTGIGLPDDRLHAPDEKLDLQQLWDGIRVFGRFYQLMGERRA"
     gene            1524..2654
                     /locus_tag="AC16JC_04845"
     CDS             1524..2654
                     /locus_tag="AC16JC_04845"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5164058.1"
                     /translation="MRTTASFDLFPDAPSERALARARWLAREGRADDADAAYRDVLTL
                     HPDFKPCWAEYFELLRAQGRAAEALHLAEAAQAQFADSAFPLALLGAALLELGRYREA
                     LAALERAVETDPDLGLVWHELGLAAYRLGDRTRALAALDRAFALEPHGETLKLRGRVL
                     RDAGRYPAAEVAFEAAAQAAEHDEQRHAAEREILTTQRYAAYAPRKPEDLQAAERWFA
                     ETGAAVLAPRTGPVPPGDEALVQALGEMAEDSGWRFGQVVAVGPTLPIWNSLADLVDA
                     PLVARADFDPAAVPLVAALRPLPRETGWGELAAAVAAQGSGLVFTIEHPAETERDAVV
                     TDVTGVLTEAGIRASAIPVVARALADVQHPSSRLATRRLRFG"
     gene            2690..>3066
                     /locus_tag="AC16JC_04850"
     CDS             2690..>3066
                     /locus_tag="AC16JC_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HIR5164059.1"
                     /translation="MSGKAIAQNHFDRAADLIGLESYMRRILTAPFREIQVEIPVRMD
                     AGRIEVFTGYRIQHNGARGPCKGGIRYHPHADHDEVLGLATIMTWKTALMDIPFGGAK
                     GGVAVDPRTLSMLELERLTRRFT"
BASE COUNT          432 a         1083 c         1122 g          429 t
ORIGIN      
        1 gtcgccgcca ggatggcgac caggatcacc gtgaccagcc aggccgtctg ctgaggcatc
       61 ggacctccag gacgcaggat cgccgccgga ggtcctaatg tggagggcgc cgcggagacc
      121 ggctaggccc gacgttcccc catcagctga tagaagcggc cgaacacccg gatgccgtcc
      181 cagagctgct gcaggtcgag cttctcgtcg ggtgcatgca gccggtcgtc cggcaggccg
      241 ataccggtga gcagcacggc cgcgccctca cggctcagcg ccggcacgac cgggatcgat
      301 ccgccggcgc gcgcggggac ggtcccccgt cccaccacct cgtgaaacgc ccggtcgagc
      361 agcgcgaacg ccgggtggtc gatcttcacc cgggccggat cgcccccgtg gaggaaccgc
      421 accgtggcgg tgacgtactt cggggcgagt ttcttgacgg ccgcggcgag ctgccgctcc
      481 accgtgcgca tcttctggtt cggcacgagc cgcaggctca ccttcgcgac cgcttcggcc
      541 gggatcacgg ttttggcgcc ggcgccggtg aacccgccgg tgatgccgtg aatctcgaac
      601 gtcggcagcg cccagagacg ctcgaggacg gagtggctct tcaggccggt cagcgccttc
      661 gcctggacgc ggcggttgag gaagtctttc tcgcggaacg ggagccgttt ccaggccgcg
      721 cgctcgcggg cggagggcgc ctgcacggcg gcgtacagtc ctggaatgtt gatccggccg
      781 ttggggccct tgagacgcac tagcaggcgg cacagctcct cgtgggcgtt gggggcggcg
      841 ccgccgtact cgccggagtg cagatcgtgc ttggccgtcc gcaccgcgag ttcggcgtag
      901 cacatgccgc gcagcgaggt gtacacggcg ggccatcccg gcgcgaggta ggacatgtcc
      961 gccaccaggg cgacgtcggc ccgggtgcgc tccggctcac gtcgcagcag gtcgaacagg
     1021 accgtgctgc cgaattcctc ctctccttcg atcaggaagc ggaagttgac gggtggggcg
     1081 ccgagcgtct cgatggcccg gagcaggcag aacacctgac ccttgtcgtc ggcggcgccg
     1141 cgggcgtaga ggcggccgtc gcgcagcgag ggctcgaacg gcggcgacgt ccattcattg
     1201 atcggatcgg gcggctgcac gtcgtagtgg ccgtagatga gcaccgtggg tgcccccggc
     1261 acgtcggggc cgacgcccca gacgaccgga tgggtctcgc tgccgagcag ctggacgtcg
     1321 cggcagccga ggcggcgcag gtcggtggcg acccactcgg cggcgcgccg gcagtccgcg
     1381 tcgtgcgccg gcagcgtgga gatcgagggg atgcggagaa acgtcgtgag ctcgccgagc
     1441 catcgttcct gttccatgcg cgacaatcta gccgggaatg ggcgcggcta gccagcgcac
     1501 ggcccgcggg tagattagcg gccatgcgca cgacggcgtc gttcgacttg tttccggatg
     1561 cgccgagcga gcgcgcgctg gcccgcgcac gctggctggc gcgcgaggga cgtgccgacg
     1621 acgcggacgc ggcgtatcgc gacgtgctca cgctgcatcc cgacttcaag ccctgttggg
     1681 cggaatactt cgagctgctg cgcgcgcaag ggcgcgcggc ggaggcgttg catctcgccg
     1741 aagcggccca ggcccagttc gccgactccg cgttcccgct cgcgttgctg ggcgccgccc
     1801 tgctcgagct ggggcgatac cgggaggcgc tggcggcact ggagcgcgcc gtcgaaacgg
     1861 atcccgatct cgggctggtg tggcacgagc tggggctcgc ggcctaccgg ctgggcgacc
     1921 ggacccgcgc gctggcggcg ctcgatcggg ccttcgccct cgagccgcat ggggagacgc
     1981 tgaagctgcg cggccgagtg ctgcgcgacg ccgggcgcta cccggcggcc gaagtggcgt
     2041 tcgaagccgc cgcgcaggcg gccgagcacg acgagcagcg ccacgcggcg gaacgggaga
     2101 tcctcacgac ccagcgctac gccgcgtatg cgccgcgcaa acccgaggat ctccaggccg
     2161 ccgaacgctg gttcgcggag acgggcgcgg cggtcctggc accgcgcacc gggcccgtcc
     2221 ctccgggcga cgaggcgctg gtccaggcgc tgggtgagat ggccgaggac tccggctggc
     2281 gcttcggaca ggtcgtcgcc gtcggtccca cgctgccgat ctggaacagc ctcgccgacc
     2341 tggtcgacgc gccgctcgtg gcccgcgccg acttcgaccc cgctgccgtt cccctcgtgg
     2401 cggcgctgcg tcccctgccg cgcgagaccg ggtggggtga gctcgccgcc gcagtggcgg
     2461 cgcaggggag cggcctggtg ttcaccatcg agcatccagc cgagaccgag cgggatgccg
     2521 tcgtcaccga cgtgaccggg gtcctcaccg aagccgggat ccgcgcgagc gccattccgg
     2581 tcgtcgcgcg cgccctggcc gatgtccagc acccttcctc ccggctcgcg acccgccgcc
     2641 tgcgattcgg ctgagccgcc ccagcgtgac cgccgtcccc ccgcccgtga tgtcgggcaa
     2701 agccatcgcg cagaaccatt tcgaccgggc ggcggacctg atcggactcg agagctacat
     2761 gcgccgcatc ctgacggcgc cgttccgtga gatccaggtg gagatcccgg tgcgcatgga
     2821 cgccggccgg atcgaggtct tcaccgggta ccgcatccag cacaacggcg cccgcggccc
     2881 ctgcaagggc ggcatccgat accatcccca cgccgaccat gacgaagtgc tgggcctcgc
     2941 cacgatcatg acctggaaga cggcgctgat ggacatcccg ttcgggggcg ccaagggcgg
     3001 cgtcgcggtc gacccgcgca cgctgtcgat gctcgagctg gagcggctca cccggcgctt
     3061 cacgca
//
LOCUS       DBNGZW010000200         4621 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_290419, whole genome shotgun sequence.
ACCESSION   DBNGZW010000200 DBNGZW010000000
VERSION     DBNGZW010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4621)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4621)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4621
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_290419"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..656
                     /locus_tag="AC16JC_04855"
     CDS             33..656
                     /locus_tag="AC16JC_04855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164060.1"
                     /translation="MPNSRRMAMLPLALSLLVPRSAVAQDPRLERRLDSATFRSVSAV
                     IDSADRQGLPVEAMVQRALEGAAKHASSAQIVAAVRRLGQDLGTARDALGVASSQPEI
                     VAAASALRAGVRVTDLTRLRQRRTQDLTVALATLADLVTSGVPPDSAAAAVIALAASA
                     RDDQIADFRRAVERDIALGAPPAAAAAVRVDAAIQQAGRTPGPARRP"
     gene            675..1814
                     /locus_tag="AC16JC_04860"
     CDS             675..1814
                     /locus_tag="AC16JC_04860"
                     /inference="COORDINATES: protein motif:HMM:NF027875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HIR5164061.1"
                     /translation="MRFGRPAWAAAFVVAYTSAAPPPVAAQTGATMDLGVTNVRYDGF
                     LPSSAASASGELRLERARVVAWTRGTYLQFESGRHSMQASIAGSLFSGPWRGLRAELS
                     GHTGLSQYADFATYSHLLATPRVHFASERTGAWAGGTAGTTWLGGARRPASGVEAGAW
                     ARRLGITWLISAASTHVGDTVYTDFQGASHVRRGRVTFDGSFGARAWSRGGGHGVYGE
                     ASGSVALNPWLSVIVSGGRYPTDPTRGSVAGRYLGVSLRMAALPHHSSFAVPVRPAAP
                     RLQSAPLDPPAASIELRSCRCFGITLAIHGAGAAQVEIAGDFTNWEPLALSLTEGDVW
                     TAVLPLASGTYRFNVRLDGGEWIVPAGVTTLSDEFGGAVGLLRVP"
     gene            complement(1824..3032)
                     /locus_tag="AC16JC_04865"
     CDS             complement(1824..3032)
                     /locus_tag="AC16JC_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008523884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5164062.1"
                     /translation="MNARRVLAVLSAAELCAMAPWFSASAVAPALAREWHLTAFGTGG
                     LTVSVQLGFVAGALLSAILTLSDIWSARRLVAGASLGAAVATVGVALSSGPAWGLGFR
                     FLTGAALAGVYPPGMKIAAGWFREARGWAIGILVGALTLGSAVPHLVRWAVPVDMWRG
                     VVLVAAASALLGGLLVLLVPNDGPFAAPSPPFSWSAAPRILRDRAVLLANLGYLGHMW
                     ELYAMWTWISAFVAASETARLGGSGTPGTTAALVTFGVVGIGAVGCWLGGKYADRLGR
                     TVVTIAAMIVSGICCLAVGPLFARPLVLLVPLLLIWGVSVVADSAQFSTAVSELAPAD
                     YVGTALTMQTSMGFLLTTITISLLPVAASAMGWRWAMSVLAVGPAVGVWAMTRLRQRP
                     EAVRLAGGRR"
     gene            complement(3029..3781)
                     /locus_tag="AC16JC_04870"
     CDS             complement(3029..3781)
                     /locus_tag="AC16JC_04870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HIR5164063.1"
                     /translation="MRLEGLVRYCDELLDTKNIRDAPEAWNGLQVANRGEVSHIAAAV
                     DLCAATVRMAGEHGADFLIVHHGLFWGGPRPLVGPQLERVAGLLRQDIALYGSHLPLD
                     CHPELGNAAVMARELGVATRGVFGTWREQPFGIWGEVDLARKDLGDRLTMLLGTAPRA
                     MLFGPAVVRRVGIATGAGGSLIPQAAAAGLDTLITGEGNHHTYFDAEELRLNVYYGGH
                     YATETFGVKALAQHLGTKFALPWVFLDHPTGL"
     gene            complement(3958..4344)
                     /locus_tag="AC16JC_04875"
     CDS             complement(3958..4344)
                     /locus_tag="AC16JC_04875"
                     /inference="COORDINATES: protein motif:HMM:NF016408.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR5164064.1"
                     /translation="MAIFTEKSGSQGGEAGLSIIGSGMRVVGDITAEGVVKVEGTVVG
                     TVQAGRQVLVAKGGLVEGDVVTHEAIIGGEVRGSVQASERVELQTTSVVHGDISTRRL
                     LVQEGGEINGVLHMGEGAPEPATSAP"
     gene            complement(4347..>4621)
                     /locus_tag="AC16JC_04880"
     CDS             complement(4347..>4621)
                     /locus_tag="AC16JC_04880"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR5164065.1"
                     /translation="PIRASGGGTVAAAATNADYGMFVLLRHPGGYESMYGHASRLLVR
                     EGDSVVAGQVIALTGSSGRSTAPHLHFEIRREGQSLDPLTLVKEGN"
BASE COUNT          751 a         1655 c         1541 g          674 t
ORIGIN      
        1 agctccgtcg tgacggtggg aggatgaccg gtatgcccaa ctcccgcaga atggccatgc
       61 tgccgctcgc gctgagcctg ctggtgccgc gctccgcggt cgctcaggat ccacggctcg
      121 agcgacggct cgacagcgcc acgtttcgat cggtgagcgc ggtgatcgat tccgccgatc
      181 ggcaggggct tcccgtggag gccatggtgc agcgggcgct cgagggggcc gccaagcacg
      241 cctcctcagc acaaatcgtc gcggcggtcc gccggcttgg ccaggatctc ggcaccgccc
      301 gggacgcgct cggtgtggcg tcctcgcagc ccgagatcgt tgctgccgcg agcgcgttgc
      361 gggcgggtgt tcgcgtgacc gatttgacac ggctgcgcca gcgccggacc caggacctca
      421 ccgtggcgct cgcgacgctc gccgatctcg tcacgagcgg tgtgccgccc gattccgccg
      481 cagcggccgt gatcgccctc gccgcatcgg cacgggacga tcagatcgca gacttccgcc
      541 gggctgtcga gcgcgacatc gccctcggcg cccctccggc agcggcggcg gccgtgcgcg
      601 tggatgcggc cattcaacag gcagggagaa cgcccgggcc ggccaggaga ccgtgatcac
      661 atgatttcca gcgcgtgagg ttcggtcgcc ccgcgtgggc ggccgccttc gtcgtcgcct
      721 acacctccgc tgcgcctccc cccgtcgcgg cgcaaaccgg ggccaccatg gacctcggcg
      781 tcacgaacgt gcgctacgac ggcttcctgc cgagtagcgc cgcctccgct tccggcgagt
      841 tacgcctcga gcgggcccgc gtcgtcgcgt ggacgcgtgg tacatacctc cagttcgaga
      901 gtggacgtca cagcatgcag gcgagtatcg cgggctcgct cttcagcgga ccctggcgcg
      961 ggctgcgcgc cgagctgtcc ggccacaccg ggctgagcca atacgcggac ttcgcgacct
     1021 actctcatct cctcgccacg ccgcgtgtgc atttcgcgag cgagcgaacg ggagcctggg
     1081 cgggtggcac ggcgggcacc acctggctgg gcggagcccg ccgaccggcg agcggtgtcg
     1141 aagccggggc gtgggcgcgc cgcctcggaa tcacctggct gatcagcgcg gcgagcacgc
     1201 acgtgggcga cacggtctac accgacttcc agggagcctc gcacgtccgc agagggcggg
     1261 tcacgttcga tgggtccttc ggtgcgcggg cgtggagccg agggggtggg catggcgtgt
     1321 acggcgaggc gagtggcagc gtcgcactca acccgtggct gtcggtcatc gtgtcgggcg
     1381 gccggtatcc gaccgaccct acgcgcggca gcgtcgcggg ccgctatctg ggcgtctccc
     1441 tgcgcatggc cgccctgccc catcacagct ccttcgccgt gccggtccgg ccagccgcgc
     1501 cgcgcctcca gtccgctcct ctcgacccac cagccgcttc tatcgagctc cgttcctgcc
     1561 gctgcttcgg cattacactc gccatccacg gcgcaggcgc cgcccaggtc gagatcgcag
     1621 gcgacttcac gaactgggag ccgctcgcgc tctctttgac agagggagac gtgtggaccg
     1681 cagtactgcc gctcgccagc ggcacctacc gcttcaatgt ccggctcgac gggggtgagt
     1741 ggatcgttcc cgccggcgtg accaccttga gcgatgagtt cggcggtgcg gtggggctgc
     1801 tgcgggtgcc ctgatccctc cgttcaacgt ctcccgcccg ccagcctgac cgcctccggt
     1861 cgctgccgca atctcgtcat cgcccagaca ccgaccgccg gccccacggc gagcacggac
     1921 atcgcccagc gccagcccat ggcggacgcc gccacgggca ggagcgagat cgtgatcgtg
     1981 gtgaggagga accccatgct cgtctgcatc gtcagcgcgg tccccacgta gtccgcggga
     2041 gccagttcgc tcacggcggt cgagaattgc gccgagtcgg cgacgaccga gacgccccag
     2101 atcagcagca agggcacgag cagcacgagc ggcctggcga acagcggccc gaccgccagg
     2161 cagcagatgc cggagacaat catcgcggcg atcgtgacca cggttcgacc gagacgatcg
     2221 gcgtactttc caccgagcca gcacccgact gccccgatcc cgacgacacc gaacgtgacg
     2281 agcgcggccg tggtgcctgg ggtaccggat ccccccagac gcgccgtctc actcgcggcc
     2341 acgaacgcgg agatccaggt ccacatggca tacagctccc acatgtgccc caggtacccg
     2401 aggttggcca gcagtacggc gcggtcccgc aagatcctcg gcgcggcgga ccaggagaat
     2461 ggcggggacg gcgcggcgaa cggcccatcg ttcggcacga gcagaacgag gagcccaccc
     2521 aggagcgccg atgcggcggc gaccagtacg acgccgcgcc acatgtccac gggaacggcc
     2581 caccggacca gatgcggtac tgccgagccg agagtcaacg cgcccaccag aattccgatc
     2641 gcccacccgc gcgcttcccg gaaccagccg gcggcgattt tcatcccggg cgggtagacg
     2701 ccggcgagcg ctgcaccggt gaggaaccgg aaccccagac cccatgcggg gcccgacgag
     2761 agcgcgacac cgacggtggc gacggccgcg ccgagcgacg cgccggcgac gaggcgccgc
     2821 gccgaccaga tgtccgacag tgtcaggatc gcggacagga gcgcgcccgc cacgaagccg
     2881 agctgcaccg agacggtcag accgccggta ccgaacgcgg tgagatgcca ctcgcgcgcc
     2941 agggcgggcg ccacggccga tgcgctgaac catggcgcca tggcgcacag ctccgctgcc
     3001 gacaggaccg cgagtacgcg ccgggcgttc acagcccggt tggatggtcg aggaagaccc
     3061 agggaagagc gaacttggtg ccgaggtgct gggcgagtgc cttcacgccg aacgtctctg
     3121 tggcgtagtg ccctccataa tagacgttga ggcgcagctc ctcggcgtcg aaataggtgt
     3181 gatggttccc ctcgccggtg atcagcgtat cgaggccggc ggccgcggcc tgtgggatga
     3241 gagaaccccc ggcaccggtc gcgatcccga cccgccgcac gaccgcggga ccaaacagca
     3301 tcgccctggg agcggttccc agaagcatcg tgagccgatc acccagatct ttgcgagcca
     3361 ggtcgacctc accccaaatc ccgaacggtt gttcccgcca cgtgccgaac actccccggg
     3421 tcgcgacgcc gagttctcgc gccatgaccg cggcgttgcc gagctcgggg tgacagtcga
     3481 gcggcaggtg agagccatac agtgcgatgt cttgccgcag cagtcctgcg acgcgttcga
     3541 gctgtgggcc caccaggggt cgggggccac cccagaagag gccgtgatgg acgatcagga
     3601 agtccgcccc gtgctcgccg gccatgcgca cggtagcggc gcagagatcg acggcggcgg
     3661 cgatgtggct gacctcaccc ctgttcgcga cctgcaggcc gttccacgcc tcaggtgcat
     3721 cacggatgtt ctttgtatcc agcagttcgt cgcagtagcg gacgaggccc tcgaggcgca
     3781 cggtgagcag gggtcgagag tggtgttggg acgtggacag tattacaaca atatcaagac
     3841 atcattcata cagttggcaa tcgggctcag gtcgaaaaca cggtgtaaag cacgtaaaca
     3901 gttgatgttg aggaatccac atcagcgcgt agacaagctg tggacaacct ctcgcactta
     3961 cggagcagag gtcgccggct ctggggcccc ctcccccatg tgcagcacac cgttgatctc
     4021 tccgccctcc tggaccagca gccgccgggt cgagatgtcg ccatgcacga cgctggtggt
     4081 ctgcagctcc acgcgctcgg aagcctggac gctcccccgc acctcgcccc cgatgatcgc
     4141 ttcgtgggtc accacatccc cttccaccag gccgcccttc gccacgagca cctgccggcc
     4201 cgcctgcacc gtgccgacca cggtgccctc gaccttcacc acgccttccg cggtgatgtc
     4261 gccgaccacc cgcatgccgg agccgatgat cgacaggccg gcttcccctc cctggctccc
     4321 ggacttctcc gtgaaaatgg ccatcgtcag ttcccctcct tcacgagcgt gagcgggtcg
     4381 agcgactgac cctcccgccg gatttcgaag tgcaagtgcg gcgccgtgga ccggcccgaa
     4441 ctgccggtca gagctatgac ctgtccggcg accaccgagt cgccttcgcg caccagaagc
     4501 cgcgaggcgt gcccgtacat gctttcatat ccgcccgggt ggcgcaagag gacgaacatg
     4561 ccgtagtccg cgttggtggc ggccgccgcc actgtcccgc ccccggaagc acggatcggc
     4621 g
//
LOCUS       DBNGZW010000201         3840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1785024, whole genome shotgun sequence.
ACCESSION   DBNGZW010000201 DBNGZW010000000
VERSION     DBNGZW010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1785024"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1091)
                     /locus_tag="AC16JC_04885"
     CDS             complement(<1..1091)
                     /locus_tag="AC16JC_04885"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPL family radical SAM protein"
                     /protein_id="HIR5164066.1"
                     /translation="MAESVQVGLGLDGTQPVARAARLPVLDERQRGTKFVPLEPRSVL
                     NSPEQTGMDFWSLNPYVGCEFGCSYCYARFTHRYVVERAHDAGRLSDAELLEFRGAHG
                     WEAFEQRIFVKQQILGALDADLRRYHRIAQAHPGGAAPIVIGTATDSYQPAERRFRIT
                     RAVLGRLAACEGLSVGIITKSPLVVRDTDVLLRLQQRNDLEVHISLTTLDVGLIRALE
                     VRSPMPAVRLRALAKLTTAGVNAGLLIAPVLPGITDDRPRLDALFAAARAAGARFVHA
                     SPLRLYPAIRERFLPLLDARFPHLAARYRRAYAGRGAAPREYAAALSRRIRALQEKYG
                     FAIRGMQGRYEKHIPAPQWSLALDAGKAM"
     gene            complement(1371..2570)
                     /locus_tag="AC16JC_04890"
     CDS             complement(1371..2570)
                     /locus_tag="AC16JC_04890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164067.1"
                     /translation="MRLAAFALATLASVALLGGCSPISSPTNTSLFLIATIDTAVGAT
                     AQTSLGVDATGRVHVAYSQDGISNLRYATCAANCAESASWSHTTVDTTSNSGLFNSFS
                     AAAGALHIVYQVYSGQGKLRYATCSANCLLTASWQTVTIDSAGNTGHDGSLAVSPAGQ
                     LHVTYLESVAVPGGRWRLKYATCAAGCTDAASWSSAILDSAVFVDASSRALAVDGSGR
                     LHLVYQKTDSIGQTPVVYATCTASCANAANWVLTGLDTDPLDHAGPVVAVDALGVPSV
                     AYWGRLGGLATINYALCAGVCTTTIGWNILPIQSLESGTSDLDQHSLVLDAASRPQMS
                     FQHGGLSYAGCVSSCTAPGAWLEFLVDPGVGGVGSSVGGLSGGGVGIAYVSPTSVGGR
                     LKFAVSN"
     gene            complement(2602..>3840)
                     /locus_tag="AC16JC_04895"
     CDS             complement(2602..>3840)
                     /locus_tag="AC16JC_04895"
                     /inference="COORDINATES: protein motif:HMM:NF012544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5164068.1"
                     /translation="YGDLAAMFPNAGGQYVYLREAISPLFGYLYGWTLFIVIQTGTIA
                     AVAVAFARFTAVFWPSLTPDLFPGLGGTINFPQPIGPILVGLSPQRLFAILSIVVLTW
                     VNVRGVRTATIIQTTLTVTKTAALGALILLGLTIGRHPEALTANFGANFWATGGFAVP
                     LTAVGAAMVGSLFSMDAWNNVGFAGSELKNPRKDLPFAMAMGVLIVVALYLLANVAYL
                     SVLPGPAIAGAPQDRVGTAALQAILGNPGLYLMAGAIMISTFGCNNGLILSGARVYYA
                     MARDNLFFHSAGRLHPRYRTPAVALVVQALWTCLLCLSGTYNQLLNFVIFAALLFYML
                     TTVGLFALRVKRPTAERPVRAPGYPWLPALYVMATGLIAVSLLIERTTRTYSGLGLAL
                     VLLGVPVYYLWRSVVRSPAA"
BASE COUNT          661 a         1231 c         1386 g          562 t
ORIGIN      
        1 tacatcgcct tcccggcatc cagcgccagg ctccactgcg gcgccgggat gtgcttctcg
       61 tatcgtccct gcattccccg aatcgcaaac ccgtacttct cctgcaacgc gcggatccgt
      121 cgcgaaagcg ccgccgcata ctcccgcggg gccgccccgc ggcccgcata cgcgcggcgg
      181 tagcgggcgg cgaggtgcgg gaatctcgca tcgagcagcg gcagaaagcg ctcccggatg
      241 gcggggtaga ggcgcagcgg actggcgtgg acaaaccggg cccctgccgc ccgcgcggcc
      301 gcgaacagcg cgtcgagtcg cgggcggtcg tccgtgatgc ccgggagtac gggcgcgatc
      361 aggaggcccg cattcacccc cgcggtggtc agctttgcca gtgccctgag tcgtaccgcg
      421 ggcatcgggg atctcacctc gagcgcgcgg atcagtccga cgtccaaggt cgtgagcgag
      481 atatggactt ccaggtcgtt gcgctgctgg agccggagca gcacgtcggt atcgcgcacg
      541 acgagcgggc tcttcgtgat gatcccgacc gacagtccct cgcacgccgc caaccgcccc
      601 agcacggcgc gcgtgatccg gaatcgccgc tcggcgggtt gataggaatc ggtggccgtg
      661 ccgatgacga tcggggccgc gccgccggga tgcgcctggg ctatgcggtg atagcggcgc
      721 aagtcggcgt cgagcgcgcc gaggatttgt tgcttgacga agatgcgctg ctcgaacgct
      781 tcccagccgt gggccccgcg gaactcgagc agctcggcgt cgctcagtcg tccggcgtcg
      841 tgcgcccgct cgaccacgta gcggtgggtg aaccgcgcgt agcagtagct gcacccgaac
      901 tcgcagccga cgtacgggtt gagcgaccag aagtccatcc ccgtttgctc cggggaattg
      961 aggaccgacc ggggttcgag cgggacgaac ttcgtgccgc gctgtcgctc gtcgaggacc
     1021 ggcaagcgag ctgcgcgcgc gactggctgc gttccatcga gtcccagacc gacctggacg
     1081 gactcagcca cagcgtgacc agccgcagct cggacaggtg acgcagcccg attggggcgc
     1141 catcggagtg ctgcactcgg ggcagttgcc ggcgccgctg gactgaggct gaggctgcag
     1201 ggcgagcgag gggcggggtt ggatggtcat gggagggcct ccacagtttc ggtatttgtt
     1261 cgggtctcac gtcatgttat ccctccagaa cacagttggc tagtgctgtc taatccactc
     1321 ccgttcactg tcttacacgc atgcccgcgc gattcgcagg ctccggcggg ctagttcgat
     1381 acggcgaatt tgaggcggcc gcccacgctc gtgggggaga catacgcgat gccaacgccg
     1441 ccgccggaga ggccgccgac cgagctcccg acgcccccga cgcccggatc gaccaggaac
     1501 tcgagccacg cgccgggcgc ggtgcagctg gacacgcagc cggcgtagga aaggcctccg
     1561 tgctgaaagc tcatctgggg gcgcgaggcg gcgtcgagga ccagggagtg ctggtcgagg
     1621 tcggacgtgc cgctttcgag gctttggatc gggaggatgt tccatccgat cgttgtggtg
     1681 cacacaccgg cacacagggc gtagttgatc gtcgccaacc caccgagcct cccccagtac
     1741 gccacactcg ggacgccgag cgcatccaca gcgacgaccg gcccggcgtg atcgagcggg
     1801 tcggtatcca gtccggtgag gacccagttc gcggcgttcg cgcagctcgc ggtgcaggtc
     1861 gcgtagacca ccggggtctg tccgatggag tcggtcttct ggtagacgag atgcagccgt
     1921 ccgctgccgt ccacggcgag cgcgcgggaa gacgcgtcca cgaacaccgc gctgtccaga
     1981 atggcgctcg accagctggc ggcatcggtg cagccggcgg cgcacgtggc atacttcagg
     2041 cgccaccgtc ctccgggaac cgcgaccgac tcgaggtagg tcacgtgcag ctgaccggcc
     2101 gggctcaccg cgagggaacc gtcgtgtccg gtgttaccgg cgctgtcgat cgtgacggtc
     2161 tgccagctcg ccgtcaacag acagttggcc gagcaggtag cgtagcggag cttcccctgc
     2221 ccgctgtaca cctgatagac gatgtgcagc gcgccggccg cggcgctgaa cgaattaaag
     2281 aggccggagt tggacgtcgt gtcgacggtg gtatgcgacc agctcgcgct ctcggcgcag
     2341 ttcgcggcgc acgtcgcata acgcagattg ctgatcccgt cctgggaata ggcgacgtgg
     2401 acccggccgg tggcgtccac gccgagggag gtctgggccg tcgcgccaac ggccgtgtcg
     2461 atggtcgcga tcagaaacag ggacgtgttg gtggggctgc tgatcgggct gcagccaccc
     2521 agcagggcga cggacgcaag cgtggccagc gcgaacgcgg caagacgcat gaggggcctc
     2581 ccgagaccgg tttctggacg gctaggcggc gggcgagcgc accaccgacc gccacaggta
     2641 gtacaccggt accccgagga ggaccagggc gaggcccagg ccgctgtagg tgcgggtggt
     2701 gcgttcgatc agcagcgaca ccgcgatcag ccccgtggcc atcacgtaga gcgccggcag
     2761 ccagggatag ccgggagccc gcaccgggcg ctccgcggtg ggacgcttga cccgcaacgc
     2821 gaacaacccc acggtcgtga gcatgtagaa cagcagcgcg gcgaagatca cgaagttcag
     2881 gagctggttg taggtgcctg acaggcacag gaggcacgtc cacagcgcct gcacgacgag
     2941 cgccacggcc ggcgtcctgt agcgcgggtg caggcgtccg gcggaatgga agaacaggtt
     3001 gtcgcgggcc atggcgtagt agacgcgggc gccggagagg atcaagccgt tgttgcaccc
     3061 gaaggtcgag atcatgatcg cccccgccat caggtagagg ccggggttgc cgaggatcgc
     3121 ctggagggcg gcggtgccga cgcggtcctg cggggcgccg gcgatcgccg gtcccgggag
     3181 cacggagagg taggcgacat tggcgaggag atacagggcg accacgatca ggacgcccat
     3241 cgccatcgcg aacggcaggt ccttgcgcgg gttcttcagc tcgctaccgg cgaaccccac
     3301 gttgttccag gcgtccatcg agaacagcga gcccaccatg gcggcgccga ccgcggtgag
     3361 tggcacggcg aacccgccag tcgcccagaa gttggcgccg aaattcgccg tgagggcttc
     3421 gggatggcgt ccgatggtga gcccgagcag gatgagcgca cccagggcgg cggtcttggt
     3481 gacggtcagc gtcgtctgga tgatggtcgc ggtgcggacc ccccggacgt tgacccaggt
     3541 cagcaccacg atggacagga tcgcgaacag ccgttgcggc gacaggccga cgaggatcgg
     3601 gccgatcggc tgcgggaaat tgatggtgcc gcccaatccc ggaaacaaat ccggagtcag
     3661 gctgggccag aacacggcgg taaagcgggc gaaggcgacg gcgacggccg cgatcgtgcc
     3721 ggtctggatg acgatgaaca gcgtccagcc gtagaggtat ccgaacagcg gggagatcgc
     3781 ttcccgcaga tagacgtact ggcccccggc gttcgggaac atggcggcga gatcgccgta
//
LOCUS       DBNGZW010000202         3900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1653468, whole genome shotgun sequence.
ACCESSION   DBNGZW010000202 DBNGZW010000000
VERSION     DBNGZW010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1653468"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..322
                     /locus_tag="AC16JC_04900"
     CDS             <1..322
                     /locus_tag="AC16JC_04900"
                     /inference="COORDINATES: protein motif:HMM:NF012485.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="secretion system protein"
                     /protein_id="HIR5164069.1"
                     /translation="FQIPSLLTRRAETDVELRDGQHLAIAGLMDNSWTKDVTRIPILG
                     DIPVLGEFFRSSAAQQRRTELLVVVTPILVDATDKAPPLPTGDAGTWDWERGLRGRPG
                     PTQH"
     gene            382..1545
                     /locus_tag="AC16JC_04905"
     CDS             382..1545
                     /locus_tag="AC16JC_04905"
                     /inference="COORDINATES: protein motif:HMM:NF024792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HIR5164070.1"
                     /translation="MTVLVAVSMLALLGFLALAIDMGMLYVAHNDAQRAADAAALAGA
                     SAFMEQDAALAEPVARARAMDYARRNTFQNGPIDSTEVSIRVVPESSLVGVRVQRDSV
                     PTLFARVFGIRHVPIGAYAAAEAVQAGNARCLKPFAVPDMWYDANGDTNLDRVWDNGE
                     TWTYGDAPGDYYLPYSGPGGSPLETGYGSDFRNSTSTQADFGRQIKIKVTNPQDVSAP
                     SPGIFLPWRIPTDSSQGQCEKGGGGGQDAGASVYRRNICTCNTSAIGLNTPYQIETGN
                     MVGPTWQGVGELINEDPNAVWDDATHSVKGSSLGDGWMSSPRVVRIALFSPGQITKSG
                     MQTIEFNNFALMFIESQNSMKDPVMGRFLYYVSGNDAPGTTTGSLVKTLKLVK"
     gene            1566..2795
                     /locus_tag="AC16JC_04910"
     CDS             1566..2795
                     /locus_tag="AC16JC_04910"
                     /inference="COORDINATES: protein motif:HMM:NF013793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR5164071.1"
                     /translation="MRSALISTDPGLRDTVRRAAALNGHGLDFAVEVATPLSEMTEEQ
                     ISELRHSDPQLVILDFDHDPETAISFARFLTDGSPQRYIVGAGPQLAAEALLEAMRAG
                     VAEYLPKPLADAGVKTALDRAARRLGCAPTSREPGQLYTLFSPKGGAGTTSVATNLAI
                     VLQRLSGKRTLLVDLDFELGEIAVLLGMQPRFSFLDLVQNFHRIDAGLLSSFIEHHES
                     GVDFLSAPFHPEPADTPGRDQIRAILHYLKRQYDYVIVDTPKSFSLDTLAAFEEADRV
                     FLVTTVDVPSLRNLQRCLPLFERVCGKDHERLRLVVNRYHPDDVISLDDVHRTVGLQV
                     YWTLSNDYEAVSRAINSGNPVARNAKSRYARDLQALGADLVGVGASTNGGGRTSSVRK
                     IGDLLGRLRRQPEKVAS"
     gene            2792..>3900
                     /locus_tag="AC16JC_04915"
     CDS             2792..>3900
                     /locus_tag="AC16JC_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014323584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HIR5164072.1"
                     /translation="MTDTARRSTGAFPAPATDGKRRSLPWQAAPGQGEAGRPTAEPTA
                     VNMSQIKTRVHRRILERLNLSNLDKLDREEVVSAITKVVQDLVAQEQLPLNLGEREQL
                     VRQVVDEIFGLGPLEILMQDPEIADILVNTYDQVFVERHGKLVPTDVRFQDDRHLFQV
                     IDRIVSAVGRRIDDSSPMVDARLPDGSRVNAIIPPLAIDGPHLSIRKFKRDALSGEDL
                     LRVGSVTQPMLEVLSGIVRARLNVLISGGTGAGKTTLLNILSSFIPTDERIVTIEDSA
                     ELQLRQPHVVRLETRSANIEGQGAVPQRMLLVNALRMRPDRIVMGEVRGAEAVDILQA
                     MNTGHDGSLTTLHANSPRDALSRLETMISMASLNLP"
BASE COUNT          660 a         1313 c         1319 g          608 t
ORIGIN      
        1 cttccagatc ccgagcctgc tgacgcgccg ggcggaaacg gacgtggagc tgcgcgacgg
       61 ccagcacctg gcgatcgcgg gcctcatgga caactcctgg accaaggacg tgactcgcat
      121 cccgatcctg ggcgatatcc cggtgctcgg cgagttcttc cgctccagcg ccgcgcagca
      181 gcgacgcacg gagctgctgg tcgtggtcac gccgattctg gtcgacgcga ccgacaaggc
      241 gccgccgctc cccaccggtg atgcgggcac ctgggactgg gaacgggggc ttcgcggccg
      301 gcccgggccc acccaacact gagcatgggg gatacgatcg tgcacgtgct gccaagggtc
      361 gcgcggtcgg agcgtggagc cgtgacggtg ctggtcgcgg tctctatgct cgcgctcctg
      421 gggttcctcg ccctggcgat cgacatgggg atgctctacg tcgcgcacaa cgacgcgcaa
      481 cgcgcggccg atgcggccgc cctggcggga gccagcgcgt tcatggagca ggacgccgcg
      541 ctggccgagc ccgtggcgcg ggcgcgcgcc atggactacg cccgccggaa cacgttccag
      601 aacgggccga tcgattcgac cgaggtgagc atccgggtgg tgcccgaaag ctccctggtg
      661 ggcgtgcgcg tgcaacgcga ctcggtgccg acgctgttcg cccgggtctt cggcatccgc
      721 cacgttccca tcggcgccta tgcggcggcg gaagcggtgc aggccgggaa cgcccgctgc
      781 ctcaagccgt tcgccgtgcc cgacatgtgg tacgacgcga acggggacac caacctggat
      841 cgcgtgtggg acaacggtga gacctggact tacggcgatg cgcccggcga ctactacctg
      901 ccctactccg ggcccggcgg atcgccgctg gaaaccggct atggcagcga cttccgcaac
      961 tcgacgtcca cccaggcgga cttcgggcgg cagatcaaga tcaaggtgac gaacccgcag
     1021 gatgtctcgg cgccgtctcc cgggatcttc cttccctggc gcatcccgac ggattcgtcg
     1081 caggggcaat gcgagaaggg cggcggcggt ggccaggacg ccggcgcgtc ggtctatcgc
     1141 cgcaacatct gcacctgcaa cacctcggcg atcgggctca acacgccgta tcagatcgag
     1201 accggcaaca tggtcgggcc gacctggcag ggcgtgggcg agctgatcaa tgaggacccg
     1261 aacgccgtct gggacgatgc gacgcacagt gtgaaaggct cgtcgttggg cgacggctgg
     1321 atgagcagcc cgcgggtcgt ccgtatcgcc ctgttctcgc cgggacagat cacgaagtcg
     1381 gggatgcaga cgatcgaatt caacaatttc gcgctcatgt tcatcgaatc gcagaactcg
     1441 atgaaggacc ccgtgatggg gcgcttcctg tactacgtct cgggcaacga tgcacccggc
     1501 accaccacag ggtcgctcgt gaagaccctc aagctcgtga aatgacggaa gggaacggta
     1561 atagcgtgcg tagtgcactg atttcgacgg accccggcct gcgggacacg gtgcggcggg
     1621 cagcggccct caacggacac ggcctcgact tcgccgtgga ggttgcgacc ccgctcagcg
     1681 aaatgaccga ggagcagatc agcgagctgc ggcacagcga tccgcagctg gtgatcctgg
     1741 acttcgacca cgacccggag acggcgatca gtttcgcccg cttcctgacg gacgggagcc
     1801 cgcagcgcta catcgtgggc gccggtccgc aacttgcggc tgaggcgttg ctggaagcca
     1861 tgcgggccgg cgtcgccgag tatctgccga aaccgctcgc ggacgccggc gtgaagaccg
     1921 cgctcgatcg ggccgcgcgg cggctcggct gcgcgccgac cagccgtgag ccggggcagc
     1981 tctatacgct gttcagcccc aagggcggtg ccggcacgac gtcggtggcc accaacctgg
     2041 ccatcgtgct gcagcgcctg agcggcaagc ggaccctgct cgtcgatctc gacttcgagc
     2101 tcggggaaat cgccgtgctg ctcggcatgc agccgcgctt cagcttcctg gacctcgtgc
     2161 agaacttcca tcgcatcgat gcggggctgc tctcctcctt catcgagcat cacgagtcgg
     2221 gcgtggattt cctctccgcg ccgttccatc cggagccggc cgataccccc ggacgcgacc
     2281 agatccgcgc gatcctgcac tatctcaagc gccaatacga ctacgtcatc gtcgacacgc
     2341 cgaagtcgtt ctccctggac actctggcgg cgttcgagga ggcggaccgc gtgttcctcg
     2401 tgacgacggt agacgtcccg tccctgcgga atctccagcg ctgtctgccg ttgttcgagc
     2461 gggtgtgcgg gaaggatcac gagcgcctgc ggctcgtcgt aaaccgctac catcccgatg
     2521 atgtgatctc gctcgacgac gtccaccgca ccgtcgggct ccaggtgtac tggacgctga
     2581 gcaacgatta cgaagcggtg agccgggcga tcaactccgg taacccggtc gcgcggaacg
     2641 ccaagtcccg ctacgcgcgg gacctccagg cgctgggtgc ggacctggtc ggcgtcggcg
     2701 cgtccacgaa cggcggcggc cgcaccagca gcgtccggaa gatcggcgac ctgctcggcc
     2761 gtctccgccg ccagcccgaa aaggtggcgt catgaccgac accgcacggc gctccaccgg
     2821 agccttcccc gcccccgcaa ccgacgggaa gcggcggtcc ctgccgtggc aggcggcgcc
     2881 cggccaggga gaggcgggtc ggccgaccgc tgaaccgaca gccgtcaaca tgtcgcagat
     2941 caagacgcga gtgcatcgcc gcatcctcga gcggctgaac ctctccaacc tcgacaagct
     3001 cgaccgggaa gaagtcgtct ccgccatcac caaggtcgtg caggacctgg tcgctcagga
     3061 gcagctgccg ctgaacctcg gcgagcggga gcagctggtg cgccaggtgg tcgacgagat
     3121 cttcggcctc gggccgctcg agatcctcat gcaggatccg gagatcgcgg acatcctggt
     3181 caacacgtac gaccaggtgt tcgtggagcg gcacggcaag ctggtcccca ccgacgtccg
     3241 ctttcaggac gatcggcacc tgttccaggt gatcgaccgg atcgtgtcgg ccgtgggccg
     3301 ccggatcgac gactcgtccc cgatggtcga tgcgcggctg ccggatggct cgcgggtcaa
     3361 cgccatcatt ccgcccctgg cgatcgacgg gccgcacctc tcgatccgaa agttcaagcg
     3421 cgacgcattg tcgggtgagg atctgctccg ggtgggaagc gtgacgcaac cgatgctcga
     3481 agtcttgagc gggatcgtgc gcgcccggtt gaacgtcctc atttcgggcg ggaccggcgc
     3541 ggggaagacg acgctgctca acatcctctc gtcctttatc ccgacggacg agcggatcgt
     3601 gacgatcgag gattccgccg agctgcagct ccggcagccg catgtcgtgc ggctggagac
     3661 gaggtcggcc aacatcgaag gtcagggcgc tgtgccgcag cgcatgctgc tggtgaacgc
     3721 cctgcgcatg cggccggacc ggatcgtgat gggcgaggtg cgcggcgccg aggcggtcga
     3781 catactgcag gcgatgaaca cggggcacga cggatcgctc accacgctgc acgcgaactc
     3841 tccgcgcgac gccttgagcc ggctcgagac gatgatctcg atggcgagtc tcaatctccc
//
LOCUS       DBNGZW010000203         5594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_497209, whole genome shotgun sequence.
ACCESSION   DBNGZW010000203 DBNGZW010000000
VERSION     DBNGZW010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5594
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_497209"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..760
                     /locus_tag="AC16JC_04920"
     CDS             <1..760
                     /locus_tag="AC16JC_04920"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5164073.1"
                     /translation="TPAHAEVGLAVLSRGIAALIEKPLADSLEGAEALVRQATQRGIV
                     LQVGHVERFNRAVRAAAPYLAEPRFLQTERLAPFQPRGTDVAVILDLMIHDLDLILEL
                     VRSEVREVRATGVPVLTPHVDIANARVEFANGAVALVTASRISRERTRKLRIFQPTGY
                     FSLDLAQGSGHFYRLKPGWQGLGARRIEDIVEAVRLEAPEAEPLKLELESFLRAVRGE
                     SEVVVSGAAGAQALALAWRVAEAIQASPLATIKP"
     gene            757..1872
                     /gene="lpxB"
                     /locus_tag="AC16JC_04925"
     CDS             757..1872
                     /gene="lpxB"
                     /locus_tag="AC16JC_04925"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HIR5164074.1"
                     /translation="MSGGPRIYVSAGEPSGDAHAAPVIAALRRQAPGVTIEAFGGPQM
                     EAAGACVLDRMEAFSVVGFVEALEGIPRHYRLLRRTRNALRAGRYDVAVFVDYPGYHL
                     RAAAAAAAAGVPVLYYIAPQLWAWGASRVSRLAAAVRRLAVILPFEEPFFRERGVPTT
                     FVGHPLKDRPPLPSRMEARRALGLAPDRPTLGVFPGSRAQEVRRIWPVARDAARRVVA
                     ARPAVQVVVAGTPVATYPDAGTIRVATGDPALVFAAADAGLCKSGTTTLEAALADLPM
                     VITYRLHPVSNFIARRVLQVPWVGLVNLVAGELVCPELLQGAAQPQALADGLLPLLDA
                     DDPATRRQRVALARVREQLGPPGAAERVATLALELTR"
     gene            1869..2498
                     /locus_tag="AC16JC_04930"
     CDS             1869..2498
                     /locus_tag="AC16JC_04930"
                     /inference="COORDINATES: protein motif:HMM:NF015961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5164075.1"
                     /translation="MRLRWAEPLLTSVGFVLVGLLARTWRYRERGREYPQALSDAKRP
                     FVFALWHSRILPLLYLHRHEAMVLLISRHRDGGLLATLAERWGYRFVRGSTQRGGEVG
                     LLGVVRALQAGNVVAITPDGPRGPAERVKPGIVAAAQHAGVSVVPAMARPSRAWWLSS
                     WDRFCIPKPFATIEVTYGPALAIGAGKDGLRAGIDAVERGLNAIMAAPR"
     gene            2495..3562
                     /gene="lpxK"
                     /locus_tag="AC16JC_04935"
     CDS             2495..3562
                     /gene="lpxK"
                     /locus_tag="AC16JC_04935"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - lipid-A 4'-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HIR5164076.1"
                     /translation="MTGDRSARLVRRLWRRRSWRARAARAVLLPFSGLYAGIVSVRAA
                     AYRAGLARDHPLPLPAIAVGNLSVGGTGKTPLAAWIAAFCVRAGRRPGILLRGYGRDE
                     ALVHERLVPEAIVVADRDRQAGGAAAQAQGADILVLDDAFQLLAVRPDLNIAVLAAEQ
                     ERLARWTLPAGPWREPRRALGRADILVVTRKRAEAESADALAGRLAARWPAATVAQAR
                     LVVRSLVGMRSGTRVSLDGLRGQQVYAAAAIADPESFAVQIRSSGATVQLAAYQDHHE
                     YDSADVRRLVRLSATAGYLVVTEKDAVKLRALWPAEGPEPFVAELEWMWERNEGAVVA
                     ALSRVLAVPGHPKRDITNRNP"
     gene            3559..4863
                     /locus_tag="AC16JC_04940"
     CDS             3559..4863
                     /locus_tag="AC16JC_04940"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016481445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR5164077.1"
                     /translation="MTDTVSQTENVIRPDKDRFLSEENPFESMMERFDHAAKLLSLDP
                     GLYKVLRHPEKQIIVSVPVVRDNGEVEVYTGYRVLYNTSRGPAKGGIRFDLNVTLEEV
                     KALAAWMTWKCAVVNIPFGGAKGGVVCDPFTMSMAELERVTRRYTASIIEILGPDSDV
                     PAPDVNTNERVMAWIMDTYSMHHRHTVTAVVTGKPVEMGGSLGRREATGRGCMIVTRE
                     ALARLGLPLQQARVAVQGFGNVGSIAADLLSKAGATIIAVADKSGGLINAKGLDIPDV
                     VAWAREHRQLAGYPRADSISNEELLTTDCDVLLPAALENVITSKNAKKIKARVICEGA
                     NGPTTATADKILEEKGVLVIPDILANAGGVTVSYFEWVQDRGGYFWDEETVNRRLEAI
                     ITRSFKEVVTLADKHKVTMRVAAYMLAIDRVATMHRLRGMYA"
     gene            4863..5288
                     /locus_tag="AC16JC_04945"
     CDS             4863..5288
                     /locus_tag="AC16JC_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004036965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HIR5164078.1"
                     /translation="MRYHVVAVGRLRNVGLRATCDEYLKRLRRYAKVEEREVKDEARL
                     PEAIPGEARLVALSRRGETWSSTELARRTSGWDQDGRDVAFAIGGAAGLPPALLRRAE
                     RVWSLSPLTFPHELARVILLEQLYRAFTIRRGEPYHRGS"
     gene            5323..>5594
                     /locus_tag="AC16JC_04950"
     CDS             5323..>5594
                     /locus_tag="AC16JC_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013988.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMMECR1 domain-containing protein"
                     /protein_id="HIR5164079.1"
                     /translation="MARRALQATLEEQPEPGPELAALPAALRGPGRAFVTLRLDDALR
                     GCIGHLASGHPLADVVWRMTLLAATEDPRFPPVGRDELPRLRIEISV"
BASE COUNT          768 a         1881 c         2086 g          859 t
ORIGIN      
        1 gacacccgcg cacgccgagg tggggctggc ggtgctgtcg cgcggcatcg ccgccctgat
       61 cgaaaagccg ctcgccgatt cgctcgaagg ggccgaggcc ctggtgcggc aggccaccca
      121 acgcggcatc gtgctgcagg ttgggcatgt ggagcgcttc aaccgcgccg tccgggccgc
      181 cgcgccgtat ctggccgagc cgcgcttcct acagaccgag cggctggcgc cgttccagcc
      241 gcgcgggacc gacgtcgcgg tgatcctcga cctgatgatt cacgacctgg atctgatact
      301 cgagctggtg cggtccgagg tccgcgaggt gcgggcgacc ggcgtgccgg tgctcacgcc
      361 gcacgtcgac atcgccaacg cgcgcgtgga gttcgccaac ggggcggtgg cgctcgtgac
      421 cgccagccgg atctcccggg agcggacccg caagctccgc atcttccagc cgacggggta
      481 cttctcgctc gatctggccc agggcagcgg gcacttctac cggttgaaac cgggatggca
      541 ggggttgggg gcgcggcgca tcgaggacat cgtcgaagcg gtgcgcctcg aggctccgga
      601 agccgaacct ctcaagctgg agctcgagag cttcctccgg gcggtgcgcg gcgagagcga
      661 agtcgttgtg tcgggggctg ccggtgcgca ggcgctggcg ctggcatggc gcgtggccga
      721 agcgatccag gcgtcgcccc tcgccaccat caagccgtga gcggcggtcc ccggatctat
      781 gtctccgccg gggagccctc gggggacgcc cacgccgcgc cggtgatcgc cgcattgcgg
      841 cggcaggcgc ccggggtcac gatcgaggcg ttcggcgggc cgcagatgga ggcggcgggc
      901 gcctgcgtgc tggatcgtat ggaagcgttc tcggttgtcg gcttcgtcga ggccctggaa
      961 gggatccccc gccactatcg tttgctgcgg aggacccgga acgcgctccg tgcgggccgc
     1021 tacgacgtgg cggtgttcgt cgactatccg ggctatcacc tgcgtgccgc ggccgcggcg
     1081 gcggcggccg gcgtgccggt gctctactac atcgcgccgc aactctgggc ctggggcgcg
     1141 agccgcgtga gtcggctcgc ggcggccgtg cgccggctgg cggtgattct gccgttcgag
     1201 gagccgttct tccgggagcg gggcgtgccg acgacgttcg tgggccatcc cttgaaggac
     1261 cggccgccgt tgccgtcccg tatggaggcg cgccgagccc tcggcctggc tcccgatcgc
     1321 cccacgctgg gcgtcttccc cggaagccgc gcccaggagg tgcgtcgcat ctggcccgtg
     1381 gcgcgggacg ccgcacgccg agtggtcgcc gcccgtcccg ccgtgcaggt ggtggtggcg
     1441 ggcacgccgg tcgctaccta cccggacgcc ggaacgattc gcgtggcgac cggggacccc
     1501 gcgctcgtgt tcgccgcggc cgacgccggg ctgtgcaaat ccggcacgac gacgttggag
     1561 gcggcgctcg ccgatctccc gatggtgatc acgtatcggc tccatcccgt atcgaacttc
     1621 atcgcccggc gcgtgctgca ggttccctgg gtcggtctcg tgaacctggt cgcaggcgag
     1681 ctcgtgtgcc cggagctgtt gcagggcgct gcgcagcctc aggcgctggc ggatgggctg
     1741 ctgccgctgc tcgatgcgga cgatccggcc acgcggcggc agcgggttgc gctggcgcgc
     1801 gtgcgggaac aactcggccc gccgggcgcg gccgaacggg tcgcgaccct ggcgctggaa
     1861 ctcacgcggt gagactccgc tgggccgagc cgctgctcac gtcggttgga ttcgtgctcg
     1921 tcggactctt agcgcggacc tggcgctatc gcgagcgcgg ccgggagtac ccgcaggcgc
     1981 tttccgacgc caagcggccc ttcgtgttcg ctctgtggca cagccgcatc ctcccgctgc
     2041 tgtacctcca tcgtcacgag gcgatggtgc tgctcatcag ccgccaccgt gacggcggcc
     2101 tcctggccac actggcggag cggtggggat accgcttcgt gcgcgggtcc acgcagcgcg
     2161 ggggcgaagt cggcctgctg ggcgtcgtcc gggcgctgca ggcgggaaac gtcgtggcga
     2221 tcacgccgga tggcccgcgc ggtccggcgg aacgcgtgaa acccggcatc gtcgcggccg
     2281 cccagcatgc gggcgtgtcg gtcgtccccg ccatggcccg gccgtcgcgc gcatggtggc
     2341 tgtcgagctg ggaccggttc tgcatcccca agcccttcgc cacgatcgaa gtaacgtacg
     2401 gaccggcgct ggccatcgga gccggcaagg acgggctacg ggccggcatc gatgcggtgg
     2461 agcggggatt gaacgccatc atggctgcgc cccggtgacc ggcgaccgga gtgcgcggct
     2521 ggtccgccgg ctctggcgtc gccgcagctg gcgtgctcgg gcggcacgtg ccgtgctcct
     2581 gccgttctcc gggctgtatg ccggaatcgt atcggtgcgg gcggccgcct atcgcgccgg
     2641 actcgctcgc gatcacccgt tgccgcttcc cgccatcgcg gtgggaaacc tgtcggtggg
     2701 cggcacgggg aagaccccgc tcgcggcctg gatcgcggcc ttctgtgtgc gcgccgggcg
     2761 gcgtcccggt atcctgctcc ggggctacgg acgcgacgaa gccctggtgc acgagcggct
     2821 cgtcccggag gcgattgtgg tcgcggatcg ggatcggcaa gccggcggcg ccgcggcgca
     2881 ggcgcagggc gcagacatcc tggtcctcga tgacgcgttc cagttgctcg cggtgcgccc
     2941 cgatctcaac atcgccgtgc tcgcggccga gcaggagcgc ctggctcgct ggaccctgcc
     3001 ggcgggtccc tggcgtgagc cgcgcagggc actggggcgc gcggacatcc tggtcgtcac
     3061 gcggaagcgg gccgaggcgg agagtgccga cgcgctggcg ggacggctcg ccgctcgctg
     3121 gccggcggcg accgtcgccc aggcacggct ggtggttcgg tccctcgtgg ggatgcgctc
     3181 cggcacccgg gtgtcgctgg acgggctccg ggggcagcag gtgtacgcgg cggccgccat
     3241 cgccgacccg gagagcttcg cggtgcaaat ccgctcctcg ggcgccacgg tgcaactcgc
     3301 ggcgtaccaa gaccatcacg agtacgactc ggccgatgta cggcgcctcg tgcggctgtc
     3361 ggccacggcc ggttatcttg tggtcaccga aaaggacgcg gtgaagctgc gcgccctgtg
     3421 gccggccgag gggccggagc cattcgttgc ggagctcgag tggatgtggg agcggaatga
     3481 aggagccgtc gtcgccgcgc tgagccgcgt gctcgccgtt cccggccacc cgaagcgcga
     3541 tatcaccaac cggaacccat gaccgacacc gtttcccaga ccgaaaacgt gatccgaccc
     3601 gacaaggatc gtttcctctc cgaagagaac ccgttcgaat cgatgatgga acgattcgac
     3661 cacgccgcca agctcctcag cctggacccg gggctgtaca aggttctgcg gcatccggaa
     3721 aaacaaatca tcgtgtccgt gccggtggtg cgggacaacg gcgaggtcga ggtttacacg
     3781 ggctaccggg tgctctacaa cacctcgcgc ggtccggcga agggcgggat tcgcttcgac
     3841 ctcaacgtca cgctggagga ggtcaaggcc ctcgctgcct ggatgacgtg gaagtgtgcg
     3901 gtggtgaaca tcccgttcgg gggggccaag ggcggggtgg tgtgcgatcc gttcacgatg
     3961 tcgatggccg agctggagcg ggtcacgcga cgctacacgg cgagcatcat cgagattctg
     4021 ggacccgatt ccgacgtccc cgcgcccgac gtgaacacca acgagcgcgt catggcctgg
     4081 atcatggaca cctactccat gcaccatcgc cacacggtga cggcggtcgt gacggggaag
     4141 ccggtcgaaa tgggcgggtc gctggggcgg cgcgaggcga ccgggcgggg ctgcatgatc
     4201 gtgacccggg aggcgctggc ccgcctggga ctgccgctgc agcaggcgcg cgtggcggtg
     4261 caggggttcg ggaacgtggg ctcgatcgcg gccgatctgc tgtccaaggc gggcgcgacc
     4321 atcatcgccg tggctgacaa gtcgggcggt ctcatcaatg ccaaggggct cgacattccc
     4381 gacgtcgtgg cctgggcccg ggagcatcgg cagctggccg gctatccccg cgccgattcg
     4441 atctccaacg aagagctgct caccacggat tgcgatgtgt tgctgccggc ggcgctggag
     4501 aacgtcatca ccagcaagaa cgcgaagaag atcaaggcgc gggtcatctg tgagggcgcc
     4561 aacggcccga ccacggccac cgccgacaag atcctcgagg agaagggggt actcgtgatc
     4621 cccgacattc tcgccaacgc cggcggggtc acggtgagtt acttcgagtg ggtgcaggac
     4681 cgcggcgggt acttctggga cgaggaaacc gtcaaccgcc gcctcgaggc gatcatcacg
     4741 cgatccttca aggaagtcgt cacgctcgcc gacaagcaca aggtgaccat gcgcgtcgcg
     4801 gcgtacatgc tggcgatcga ccgggtcgcc acgatgcacc gactgcgcgg gatgtatgcc
     4861 tgatgcgcta tcacgtcgtc gcggtggggc gcctcaggaa tgtggggctg cgggcgacgt
     4921 gtgacgaata cctgaaacgg ctgcgccgct acgcgaaggt ggaggagcgg gaggtgaagg
     4981 acgaggcgcg tttgcccgag gcgatccccg gggaggcgcg cctcgtcgcg ctgtcgcgcc
     5041 gcggcgaaac gtggagctcg accgagctgg cccggcgcac gtcaggctgg gatcaggatg
     5101 gccgggacgt ggccttcgcc atcggtggcg ccgccggcct gccgccggcg ctgctgcggc
     5161 gcgcggagcg cgtctggagc ctgtcgccgc tcacgttccc ccacgagctg gcccgggtga
     5221 tcctgctcga gcagctgtac cgggccttca cgatccgccg cggggagccc taccatcgcg
     5281 gttcctgagc tgggggacga ggaacggcgg acgctgctgc gcctggcccg tagggcgctc
     5341 caggcgacgc tggaggagca gccggagcct ggccccgagc tggcggcgct cccggccgcc
     5401 ctccgcggcc cggggcgggc gttcgtgacg ttgcggctcg atgacgcatt gcggggctgc
     5461 atcggccacc tggcctccgg gcacccgctc gccgatgtgg tctggcgcat gacgctcctc
     5521 gcggccaccg aggatccccg gttcccgccc gttgggagag acgagctgcc ccggctgcgg
     5581 atcgagatct cggt
//
LOCUS       DBNGZW010000204         4097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_695297, whole genome shotgun sequence.
ACCESSION   DBNGZW010000204 DBNGZW010000000
VERSION     DBNGZW010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4097
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_695297"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1534)
                     /locus_tag="AC16JC_04955"
     CDS             complement(<1..1534)
                     /locus_tag="AC16JC_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006192635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit B"
                     /protein_id="HIR5164080.1"
                     /translation="MAKPKKTTPAEKHNGNGGYQADSIQVLKGLEAVRRRPAMYIGSV
                     SGRGLHHLVYEVVDNSVDEALAGYCTKIDATIHEDNSITVVDNGRGIPVDIHKTEKKP
                     GVEVALTVLHAGGKFDKSTYKVSGGLHGVGVSVVNALSKKLEVWVDRDGKRHHMAFER
                     GDTKKKLEVTGKVKGTGTTVWFLPDDGIFTETRFDFATLATRLRELAFLNKGLQITLT
                     DERKGSKKQETYLYKGGLVEFVKWLLGSRKPLHQKVISFEATREGVEVEGAIQYDDGY
                     NENTFSFVNNINTHEGGTHLTGFKAALTRTINEWAKKDGLLKKEDFTLTGDDIREGLT
                     AVLHVKVKDPQFEGQTKTKLGNSEVEGIVKSVVNEALGDFLDKNPSVARAVIAKAVSA
                     ARAREAARKARELVRRKNALDSGMLPGKLADCSLTDPSLCEVYLVEGDSAGGSAKQGR
                     DRKFQAILPIRGKLINVEKARLDKVLQNNEIRTMITAIGTGIGDGEGEGAFNLEKLRY
                     HKI"
     gene            complement(1546..1854)
                     /locus_tag="AC16JC_04960"
     CDS             complement(1546..1854)
                     /locus_tag="AC16JC_04960"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR5164081.1"
                     /translation="MTDSKKGRRAPVAVGEALQGFLTRSGLGSRIAQAQVIAEWATLV
                     GPQIAAVTTPESVAPDGTLFVRVATSAWRNELQLMMPDLMARVNAGRGAGRIKTIRWL
                     "
     gene            complement(1982..2365)
                     /locus_tag="AC16JC_04965"
     CDS             complement(1982..2365)
                     /locus_tag="AC16JC_04965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164082.1"
                     /translation="MTRWAARIAVLAAGVALSALACQTDRDRPGPPRLTITLAQDSVQ
                     SPDTLTGTLRAEDVDGVDSVWLSVDSAPPLGADGLLDQTFLATFRADVPGGHVLGDRV
                     SVQFTARDISGFVGVLDTFVVVKGP"
     gene            complement(2362..3144)
                     /locus_tag="AC16JC_04970"
     CDS             complement(2362..3144)
                     /locus_tag="AC16JC_04970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR5164083.1"
                     /translation="MNYQTLLFETRDGIAFVTVNRPDKLNALNDQVMAELADAAERIA
                     TTAEIRGALITGAGPKAFIAGADIGDLSRQGPFDGKARAMRGQAVLRRLETCGKPVIA
                     AVNGYALGGGCELAMACHLRIASDTAKFGQPEVRLGIAPGYGGTQRLPRLVGKGVALQ
                     LILTGEMIDAQEAYRIGLVNKVVPAADLLAESEKLLRGILAMAPLAVRLCLEAVDQGF
                     EMTLDEGLLLEANHFGLLAATNDMREGTQAFLEKRPPRFEGR"
     gene            3281..>4097
                     /gene="mtnA"
                     /locus_tag="AC16JC_04975"
     CDS             3281..>4097
                     /gene="mtnA"
                     /locus_tag="AC16JC_04975"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0033353 - L-methionine cycle [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HIR5164084.1"
                     /translation="MPVQAISWAPSGAARIVDQRALPDTLVHRDLESIDAMAEAIRSL
                     QVRGAPLIGIAAAMGLVAGLRDACGVSREEFLARVERGAALLASTRPTAVNLRWALER
                     MSTVARGTAGGGAELWERLHTEASAIWDEDRAMCQRIGEAGLPLIPEGANVLTHCNAG
                     ALATGGIGTALAPVYLAHDAGRRVHVFADETRPVLQGARLTAWELTAAHVPCTLIADG
                     VAASLMRAGRVNLVLVGADRIARNGDFANKIGTYPLAVLALHHGVPFYCCAPSS"
BASE COUNT          583 a         1421 c         1340 g          753 t
ORIGIN      
        1 tgatcttgtg gtagcgcagt ttttcgagat tgaacgcgcc ttcaccttcg ccatcgccga
       61 tgccggtgcc gatggcggtg atcatcgtcc ggatttcgtt gttctgaagc accttgtcca
      121 ggcgcgcctt ctctacattg atgagtttgc ctcgaatcgg gagaatggcc tgaaacttac
      181 gatcccggcc ttgcttggcc gagccgcctg cggagtcgcc ctcgacgagg tagacctcgc
      241 acaaactggg atcggtgagc gagcagtcgg cgagcttgcc ggggagcatg ccgctatcca
      301 gcgcgttctt gcgccgcacc agttcgcgcg ccttgcgtgc cgcctcgcgg gcgcgcgccg
      361 cgctcaccgc cttggcgatc accgcccggg cgacgctcgg gttcttgtcg agaaagtcgc
      421 ccagcgcctc gttcacgaca ctcttgacga tgccctcgac ttcggaattg ccgagcttcg
      481 tcttggtctg tccctcgaac tgcgggtctt tcaccttcac gtgcagcacg gcggtgaggc
      541 cctcgcggat gtcgtcgccc gtgagggtga agtcctcctt cttgaggagt ccgtccttct
      601 tcgcccactc gttgatggtg cgcgtgagcg ccgccttgaa ccccgtgagg tgcgtgccgc
      661 cctcgtgggt gttgatgttg ttgacgaacg agaaggtgtt ctcgttgtag ccgtcgtcgt
      721 actggatggc tccctcgacc tcgacgcctt cccgcgtggc ctcgaagctg atcaccttct
      781 ggtgcagcgg cttgcggctg ccgagcagcc acttcacgaa ctcgaccagg ccgcccttgt
      841 agaggtaggt ctcctgtttc ttcgagccct tccgctcgtc ggtgagcgtg atctgcaggc
      901 ccttgttcag gaacgccagc tcgcgcagcc gcgtggccag cgtcgcgaag tcgaagcggg
      961 tctcggtgaa gatgccgtcg tcgggcagga accacaccgt cgtccccgtg cccttcacct
     1021 tgccggtcac ctcgagcttc ttcttcgtgt cgccgcgctc gaacgccata tggtggcgct
     1081 tgccgtcgcg gtccacccac acctcgagct tcttcgacag ggcgttgacc accgagacgc
     1141 ccacgccgtg cagtccgcca gagaccttgt acgtgctctt gtcgaatttt ccgccggcgt
     1201 gcagcacggt gagggcgact tcgacgcccg gcttcttctc ggtcttgtgg atgtcgaccg
     1261 gaatgccgcg cccgttgtcc acgacggtga tcgaattgtc ctcgtggatg gtggcgtcga
     1321 tcttggtgca gtagccggcg agtgcttcgt ccacggagtt gtccaccacc tcgtacacca
     1381 gatggtgcaa cccccggccc gacaccgagc cgatgtacat cgcgggacgc cgccgcaccg
     1441 cctccaggcc cttcagcacc tggatcgaat cggcctggta gccgccgttg ccgttgtgct
     1501 tctcggcagg agtcgtcttc ttcggtttcg ccatggggta ccgtgctaga gccagcggat
     1561 cgtcttgatc cggcccgcgc cgcggcccgc gttcacgcgc gccatcaggt cgggcatcat
     1621 gagctgcagt tcgtttctcc atgcgctggt cgccacccgc acgaacagcg tgccgtccgg
     1681 tgcgacactt tccggcgtcg tcacggccgc gatttgcggc cccacgagcg tcgcccattc
     1741 ggcgatcacc tgggcctggg cgatgcggga cccgagcccg gagcgggtga gaaagccctg
     1801 gagggcctca cccacggcga ccggcgcgcg tcggcctttc ttggaatccg tcattctgga
     1861 ggggcttcga gtgtcctcgg aaagcccttc caaaataccc ggaaattgtt gtcgggtcaa
     1921 tacttagttg cgttggagcg aggccggatt tgatgcgctg acaagggctt ggcacccccc
     1981 ctcaggggcc tttcacgacc acgaaggtgt cgagcactcc cacgaagccc gagatgtccc
     2041 gcgccgtgaa ctgcaccgac acccggtcgc ccagcacgtg gccgccgggt acgtcggcgc
     2101 ggaacgtggc caggaaggtc tggtcgagca gcccgtccgc acccagcggc ggcgccgagt
     2161 cgaccgagag ccagaccgaa tcgaccccat ccacatcctc ggcccgcagc gtgccggtga
     2221 gcgtgtctgg agattgaacg gaatcctggg ccagcgtgat cgtcaggcgg ggtgggccgg
     2281 gtcggtcgcg gtcggtctga caggcgaggg cggacaacgc gacgccggcc gcgagcaccg
     2341 cgatccgggc agcccagcgc gtcatcgccc ctcgaatcgg gggggccgct tctccaggaa
     2401 cgcctgtgtc ccttccctca tatcgttcgt ggccgcgagc agcccaaagt ggttcgcctc
     2461 gagcaacagt ccctcgtcca gcgtcatttc gaagccctgg tccaccgctt ccaggcacag
     2521 ccggacggcg agcggggcca tcgcgaggat accccgcagc agcttctcgg attcggcgag
     2581 caggtccgcc gccggcacca ccttgttgac cagcccgatg cggtacgcct cctgggcgtc
     2641 gatcatctcc ccggtgagga tcagctgcaa ggcgacgccc ttgccgacga gccgcggcag
     2701 gcgctgggtg ccgccgtagc ctggggcgat cccgagcctg acctcgggct gtccgaactt
     2761 ggccgtgtcg ctggcgatcc gcaggtgaca cgccatggcc agctcgcacc cgccccccag
     2821 cgcgtaaccg ttgaccgcgg cgatcaccgg cttcccgcag gtctccagcc ggcgcagcac
     2881 ggcctgcccg cgcatcgcgc gggccttgcc gtcgaacggg ccctggcgcg acagatcccc
     2941 gatgtcggcg ccggcgatga acgccttcgg gccggcgccg gtgatgagcg ccccgcggat
     3001 ctcggccgtg gtggcgatcc gctcggccgc gtcggccagc tcggccatca cctggtcgtt
     3061 cagcgcgttg agcttgtcgg ggcggttgac ggtgacgaag gcgatcccgt cacgggtttc
     3121 gaacagcagg gtctggtagt tcatggggca atgctaattg cggattgcgg attgcggaat
     3181 gtggatttgc gcgggcgact cgggaccccc ggatcgtgcc tcgaactcgg ctccgccatt
     3241 ccgcaatccg caatcaccaa tccgcaatta gtatttgtcc atgcccgtcc aagcgatcag
     3301 ctgggccccc tccggcgccg cccgcattgt cgaccagcgc gcgctccccg acaccctcgt
     3361 ccaccgtgac ctcgagagca tcgatgcgat ggccgaggcg atccggtcgt tgcaggtgcg
     3421 cggggcgccg ctgatcggca tcgccgcggc gatggggctc gtggccggcc tgcgcgacgc
     3481 gtgcggcgtc tcgcgcgagg agtttctcgc tcgcgtggag cggggtgcgg cgctcctcgc
     3541 ctccacgcgt ccgaccgccg tcaacctccg ctgggcgctc gaacggatga gcacagtcgc
     3601 ccgcggcacg gccggcgggg gtgccgagct atgggagcgc ctccacaccg aagcgtccgc
     3661 gatctgggat gaagaccgcg ccatgtgcca acgcatcggt gaggcggggc tgccgctcat
     3721 tcccgagggc gcgaacgtcc tcacccactg caacgccggc gcgctggcga cgggcggcat
     3781 cggtaccgcg ctcgcgccgg tatacctggc gcatgacgcg ggacggcggg tgcacgtgtt
     3841 cgccgacgag acccggcccg tgttgcaggg cgcccggctg accgcctggg agctgacggc
     3901 ggcgcacgtg ccgtgcacgc tgatcgccga cggcgtggcg gcgagtctca tgcgggccgg
     3961 tcgcgtcaac ctggtcctgg tcggcgccga ccgcatcgcg agaaacgggg atttcgccaa
     4021 caagatcggc acctacccgc tcgccgtgct ggcgctccac cacggcgtcc cgttctactg
     4081 ctgcgcccct tcctcca
//
LOCUS       DBNGZW010000205         5780 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1785319, whole genome shotgun sequence.
ACCESSION   DBNGZW010000205 DBNGZW010000000
VERSION     DBNGZW010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5780)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1785319"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2011)
                     /locus_tag="AC16JC_04980"
     CDS             complement(<1..2011)
                     /locus_tag="AC16JC_04980"
                     /inference="COORDINATES: protein motif:HMM:NF037085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBG domain-containing protein"
                     /protein_id="HIR5164085.1"
                     /translation="MRRLQLLAVVVIASLFACNTDSRDLLAPSRPSGPMAAVGIDPAS
                     GATIETNQDDYLPGELVRVTGHGWASNETVHFDMTEDPDTHGDMTADVQADSAGAFDV
                     PFYDVQDHDLGVTFTLTATGLTSGSKATATFTDGNIKFTTSPVNLAPSALFDRFNGNG
                     TCSGTASASGQTLAYNTGVGLNTGQSIRVTVPTVAGQSYTGYANPNGLTITPASSASA
                     TVLCITGTVPSSQAQFVLNYVAAKQNQTISFDTPADRTFGGPPFPLSASASSGLAVSF
                     SSLTPVACSVAGSTVTILQAGSCTIRASQAGDDGYNPAPDEDRSFTIARAAVTATAGS
                     GSGTYTGAVQAPSACVVTGAFTGDLSCVNSPTTAGPDAGTTAISASVSGTGLSNYDIT
                     LVDGSYTIARASSSTAVTCPASVTYNGSAQAPCSAGVTSVGLHQSLVVSYEDNTNAGT
                     ATASASYAGDANHEGSSDSQTFTIERAPVTATAGSGSGTYDGSAQTPSPCAVTGAYTG
                     NLGCSNSPEAVGPGAGTTAISAVVNGTGLSNFAVTTVDGSYTIDKAPLTVAAEDKSKM
                     FDGAPYSPFTAAISGFVNGEDASVVSGTPGFAGPAVTAIYPGTYGITPTIGSLNAANY
                     QFASFINATLTIRAWTLSGFYQPVDMSINGTVWNTVKNGSTVPLKF"
     gene            complement(2195..3256)
                     /locus_tag="AC16JC_04985"
     CDS             complement(2195..3256)
                     /locus_tag="AC16JC_04985"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR5164086.1"
                     /translation="MDNLAHAMVGAALGRAVAGRKVRAAGWIGAVAANAPDWTEPFLG
                     FRPFRGSLGYYTLHRGITHSFVGAAVESAAITGVLWGLLVLLRRRIGPVSLLPLAALV
                     TVAVWSHIYMDWQGSYGLRPWLPWSGRWYYADWVAIVDPLFWVIPLVGLAWGAERHWM
                     ALLPVLVAAGFVLWAEFIWRSNVPAPWVRAVSAALVALVAVGWVRHWFGVAGRRAAAA
                     WSLAALAAYAGMQAVASGPAKAEVHREAVARFGPDATWAALTRVGAPFQWEPIYASHD
                     SVAGPGWVLPRNLDDARVRRVLQQTTEGRALNGFARFLAAEVDSTRPDVVVTLRDARY
                     ARTGAHGWGVLTVTADSPR"
     gene            3500..5566
                     /locus_tag="AC16JC_04990"
     CDS             3500..5566
                     /locus_tag="AC16JC_04990"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR5164087.1"
                     /translation="MPHAVFRVNAGKSHPICLPERCAVAAMPYLKLQLMSTGRILELH
                     DTVVLLGRDAAATIPFMGDDARVVSTRHAEVRPAGEGWQLADLGSRNGTFLNGRRVQG
                     EAPLTAGDEVRLGETGPRIGVLAVSQASIPAATLAELQPPQLTPTPERRGPLPPEARP
                     YGVTLIAIATGKRFEAQGTRIRIGRGKECEVRAVEGGEGIVSRVHAELTVGPGGGLSL
                     RDVGSKNGTFLNGDRIAEPVPVRLGDRITLGPGGPVLVVEGIGTGPMRAAVRPPMAPA
                     MGHNTVMGLINNALAQAREEKRRGGRGSTAFFKAVAEEVGRDSRRKFRWLTALVAVLV
                     LLLGGAVYGVYWLLSQQVEQTEVERRSAEDSARASAERLRHELEAARAAAAPAATVDS
                     LRAQLESAQVRTTELRAALERAQGALGSQLAQGESQRLASQRELERLRDQLNQAQRTA
                     PSSGTIDSLRRQMAVTEAQAASLDARMRAIRGTDFAAIAQQNQGAVGLITTRFGRNVY
                     NGTGFVIAPDGYMLTNWHVVTDSGYTRPDTMWVTMADQSTSRLADVIASSQERDIALI
                     KIRGYQGPYLASVDWGGTKSRQGEPAALIGYPAGSGFAHLRSAVVRTSMTAGIISRVT
                     EDVIQFDGMTIGGSSGSPVFNANGEVISIHRAGLPQAPGFALSVPMHHAVPLLPPEVK
                     QRLAIP"
BASE COUNT          921 a         1944 c         2024 g          891 t
ORIGIN      
        1 cgaacttcag cggtacggta gagccgttct tcacggtatt ccagaccgtg ccgttgatgg
       61 acatgtcgac cggctggtag aacccgctca acgtccaggc ccggatcgtc agcgtcgcgt
      121 tgatgaagct cgcaaactgg taatttgcgg cgttcaggga gccgatcgtg ggcgtgatgc
      181 cgtacgtgcc cggatagatt gcggtcaccg ccgggcccgc aaacccgggt gtgccgctca
      241 ccaccgacgc gtcttcaccg ttcacgaagc cgctgatggc ggcggtgaac ggcgagtacg
      301 gtgcgccgtc gaacatcttg gacttgtctt cggcggccac ggtcagcgga gccttgtcga
      361 tcgtgtacga gccgtcgacc gtggtaacgg cgaagttgct cagccccgtc ccgttgacca
      421 ccgcgctgat cgcggtcgta cccgcaccgg ggccgacggc ttccgggctg ttgctgcagc
      481 ccaggtttcc ggtgtacgcc cccgtcaccg cgcagggcga cggcgtctgc gcggacccgt
      541 cataggtccc cgacccgctg ccggccgtcg ccgttaccgg cgcgcgctcg atcgtgaagg
      601 tttgcgagtc gctcgagccc tcgtggttgg catccccggc atagctcgcg cttgccgtcg
      661 cggtgccggc gttggtgttg tcctcgtagc tcaccaccag gctctgatgc agtcccaccg
      721 atgtcacgcc ggccgagcag ggagcctgcg ccgagccgtt gtaggtcacg ctcgccgggc
      781 aggtcacggc tgtgctcgat gaggcccgtg caatggtgta cgacccatcg accagcgtga
      841 tgtcgtagtt gctcaatccg gttccgctca ccgatgcgct gatggccgtc gtccccgcat
      901 ccggtcctgc cgtggtcggg ctgttgacgc agctcaggtc tccggtgaac gcgcccgtca
      961 ccacgcaggc cgagggcgcc tgcacggctc cggtgtaggt tcccgagccg cttcccgccg
     1021 tcgccgtcac cgccgcgcgc gcgatcgtga acgaccgatc ttcgtcgggg gccgggttgt
     1081 aaccgtcgtc gcctgcctgg gaagcccgaa tggtgcagct cccggcttgc aggatcgtca
     1141 ccgtcgagcc cgcgacgctg catgccaccg gcgtcaggct gctgaaactc accgcgagcc
     1201 cggagctcgc cgaggcgctc agggggaacg gcggacctcc gaaggtccgg tccgccggcg
     1261 tatcgaagct gatggtctgg ttctgtttgg ccgcgacgta gttcagcacg aactgcgcct
     1321 gagaactcgg gacagtgccg gtaatgcaga ggacggtagc ggatgccgag gaggccggag
     1381 tgatcgtcag gccattggga ttcgcgtagc cggtgtaact ctggccggcc accgtcggca
     1441 cggtcactct gatggactgg ccggtgttca gcccgacccc cgtgttgtac gccagcgtct
     1501 gacccgaggc ggaggcggta ccggaacagg tgccgttgcc gttgaatcgg tcgaagagag
     1561 ccgatggggc caggttgacc ggcgacgtgg tgaacttgat gttgccgtcg gtgaaggtcg
     1621 ccgtcgcctt gcttcccgac gtcagtcccg tcgccgtcag ggtgaaggtc acgccgaggt
     1681 cgtgatcctg gacgtcgtag aacggcacgt cgaacgcgcc agcgctgtcg gcctggacgt
     1741 ccgcggtcat atcgccgtgt gtgtccggat cttcggtcat gtcgaagtgc acggtctcgt
     1801 tcgacgccca gccgtggccg gtgacacgga ccagctctcc cggcaggtaa tcatcctggt
     1861 tcgtctcgat cgtggcgccg ctcgccgggt cgatgccgac cgcggccatc gggccgctgg
     1921 gacgcgacgg cgcgaggaga tctcgcgagt cggtattgca tgcgaacaga gacgcgatca
     1981 ccaccaccgc cagcagctgc agcctgcgca tatgtgctcc acatcacggg aacgagtgcc
     2041 tccacctcga caagggggaa ggcaacgcgc gtgccgcgat gcggacctgg atgcgtctgc
     2101 acctgaccga gcgccggtgc gggagggctg cgcgagtgga gacgtactgc cacaggaact
     2161 gtggcggcgt ctccgtgacg agactcacat tcgatcagcg tggggaatcg gcggtgaccg
     2221 tcagcactcc ccacccgtgc gctccggtac gcgcataacg ggcgtcccgg agggtcacca
     2281 cgacgtcggg gcgagtggaa tcgacttcgg cggcgaggaa gcgcgcgaac ccgttcagcg
     2341 cccgcccttc cgtggtctgc tggagaacgc gccgcacgcg cgcgtcgtcg aggttgcgtg
     2401 gcaggaccca gccgggaccc gccacggagt cgtgcgaggc atagatcggt tcccactgga
     2461 acggcgcgcc gacgcgggtc aacgccgccc acgtcgcgtc cgggccgaat cgggcgacgg
     2521 cttcgcggtg cacctcggct ttcgcgggtc cgctggccac cgcctgcatc cccgcgtacg
     2581 cggcaagcgc ggcgagcgac catgcggcgg ccgcccgtcg tcccgccacg ccgaaccagt
     2641 ggcgcaccca gcccaccgcc acgagcgcga cgagcgcggc actcacggcg cgcacccatg
     2701 gggccggcac gttcgagcgc cagatgaatt ccgcccacag cacgaaaccc gcggcgacca
     2761 gcaccggcag cagcgccatc cagtgccgct cggcgcccca ggcgaggccg accagcggaa
     2821 tgacccagaa cagcggatcc acgatcgcca cccagtcggc gtagtaccag cggccgctcc
     2881 agggcagcca ggggcgcagc ccgtaggacc cctgccaatc catgtagatg tggctccaga
     2941 ccgccacggt gaccagggcg gcgagcggga ggagcgagac cggtccgatg cgccgccgca
     3001 acaggacgag gagtccccac agcacacccg tgatcgccgc cgactcgacg gcggctccaa
     3061 cgaaggaatg ggtgatcccg cgatggagcg tgtagtaccc gagcgatccc cgaaaggggc
     3121 ggaacccgag gaacggctcg gtccagtcgg gcgcgttggc ggcgacggcg ccgatccatc
     3181 cggcggcccg taccttgcgg cccgccacgg cgcggcccag ggcggctccc accatggcat
     3241 gggcgaggtt gtccacgggc tgggaagata gccccgtccg ccctagccgt ggcggggccg
     3301 gggtgtaggt taggcggcgt gtcggcctcg gtcccccaca cccgtcgcga gcggcgtcgc
     3361 acaccagagc gccgcgctct gatggaccgg cgctccggag tggatcggcg cggccgtgcc
     3421 gaacgtcggc gggcgaatgt cgcggttgcc gaggagcgcc gcggcgatgt ggaccgccgt
     3481 tcgggggtgg atcggcgcga tgccacacgc cgttttccgg gtgaacgccg ggaagagcca
     3541 cccgatctgc cttcctgagc gctgcgctgt agccgcaatg ccgtatctca aactccagct
     3601 gatgtcgacc ggtcgcatcc tcgaactgca cgacaccgtt gtgctgctgg gacgcgatgc
     3661 cgccgcgacg atccccttca tgggcgacga tgcccgcgtc gtctccaccc ggcacgccga
     3721 ggtgcgcccg gcgggggagg ggtggcagct ggcggacctc ggcagccgca acgggacctt
     3781 cctcaacggc cgccgagtcc aaggcgaggc accgctcacc gccggggacg aggtgcgcct
     3841 gggggaaacg gggccgcgta tcggcgtgct cgccgtgagt caggcgtcga tccccgcggc
     3901 gacgctcgcc gagctccagc cgccgcaact caccccgaca cccgaacggc gcgggccttt
     3961 gccgccggaa gcccgtccct atggggtgac cctgatcgcg atcgccacgg gaaagcgctt
     4021 cgaggcgcag ggcacgcgca tccggatcgg ccgcggcaag gagtgcgaag tccgcgcggt
     4081 cgagggcggc gagggaatcg tctcgcgggt gcacgccgag ctgacggtgg ggccgggcgg
     4141 cggactgtcg ctgcgggacg tcgggagcaa gaacgggacg tttctgaacg gcgatcgcat
     4201 tgccgagcct gtgccggttc ggctgggcga ccgcatcacg ctgggacccg gcggacccgt
     4261 cctggtcgtc gagggaatcg gcaccgggcc catgagggcg gcggtgcgac ccccgatggc
     4321 gccggcgatg gggcacaaca cggtcatggg gctgatcaac aacgccctgg cgcaggcgcg
     4381 tgaggagaag cgccgcggcg gacggggctc gacggccttc ttcaaggcgg tggcggagga
     4441 agtcggcagg gattcccggc gtaaattccg ctggctgacc gcgttggtcg ctgtgctggt
     4501 gctgctgctc ggcggggccg tgtacggggt ctattggctc ctgtcccagc aggtcgagca
     4561 gaccgaggtg gagcgtcgga gcgcggaaga ctcggcccgg gcgagcgccg agcgactgcg
     4621 ccacgagctg gaggcggccc gcgcggcggc ggcaccggcc gccacggtgg acagtctgcg
     4681 cgcccagctc gaatcggcgc aggtgcggac gaccgagctg cgcgcggcgc tcgagcgggc
     4741 gcaaggagca cttggctcac agctggcgca aggcgagtcc cagcgactcg cgtcgcaacg
     4801 cgagctggag cgcctgcgcg accagctgaa ccaggcgcaa cggaccgctc cttcgtcggg
     4861 cacgatcgac agcctgcgcc ggcagatggc agtgaccgag gcgcaggccg ccagtctgga
     4921 cgcccggatg cgagcgattc gcggcaccga cttcgcggcg atcgcgcagc agaaccaggg
     4981 agcggtcggg ctcatcacga cgcggttcgg caggaacgtc tacaacggga ccggcttcgt
     5041 catcgccccc gacgggtaca tgctcaccaa ctggcacgtc gtgaccgaca gcggctacac
     5101 gcggccggac accatgtggg tgacgatggc ggaccagtcg acctctcgcc tggccgacgt
     5161 catcgccagt tcccaggaac gcgacatcgc cctcatcaag attcgcggct accaggggcc
     5221 gtatctggcg agcgtcgatt ggggcggcac caagtcgcga cagggcgaac cggcggccct
     5281 gatcgggtat ccggcgggat cggggttcgc tcacctgcgc tctgcggtcg tccgcacatc
     5341 catgacggcg gggatcatct cccgggtcac cgaggatgtg atccagttcg acggcatgac
     5401 gatcggggga tcctccggca gccccgtctt caatgccaac ggtgaggtga tctcgattca
     5461 ccgcgccggc ctgccccagg cgcccgggtt tgcgctgtcg gtccccatgc accatgccgt
     5521 ccccctgctg ccgcccgagg tgaagcaacg gctcgctatc ccctaggaac tagctgttgc
     5581 agagtcggcc acttgcctgc aatccgccat aatcgctaaa acatgtaata tcatcatacc
     5641 gcatttcgtg tgacccatga cccagcacat ggttcagccc ccgccggtgt gtgcaagtca
     5701 tttatctatt gttgtttaga tagcagactt gtataatggg cggtctttca gtgtcaaaaa
     5761 acaagacggt tcgtgggaag
//
LOCUS       DBNGZW010000206         4319 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1179232, whole genome shotgun sequence.
ACCESSION   DBNGZW010000206 DBNGZW010000000
VERSION     DBNGZW010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4319)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4319)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4319
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1179232"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1328)
                     /locus_tag="AC16JC_04995"
     CDS             complement(<1..1328)
                     /locus_tag="AC16JC_04995"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5164088.1"
                     /translation="MTVEGLRASLADRYRIERELGAGGMATVYLAEDVKHHRKVAIKV
                     LRSELASVLGPERFLREIETTANLRHPHILPLYDSGEADGFLYYVMPLVEGESLRDRL
                     NRQKQLPIDEALAIAREVADALGYAHARGVVHRDIKPENILLEGGHAVVTDFGIARAV
                     SAAGAARLTETGITVGTPMYMSPEQAAGDADLDGRSDLYSLGCVLYEMLGGQPPFTGP
                     TAQAITHQHLVTEAAPVTNLRPTVPAEVAGALARTLAKNPADRFNPAAQFVQALSTAN
                     VAPSPRPQAGRRRFARLGVLAVGVVALGAVAWAGGRALRSTGPSGAIQRIAVLPMDNE
                     TGDSGQTYFADGMTREVIGVLTDAGVRVLGHRAVAAYRNTTQSASEIARALGVDAIVT
                     GAVLKAGDVVQVSAELTDPRTGESLWARTFSRPAPDVVTLQHEVALEIARG"
     gene            1524..3965
                     /locus_tag="AC16JC_05000"
     CDS             1524..3965
                     /locus_tag="AC16JC_05000"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5164089.1"
                     /translation="MPDLLRQQVRYAARGLTRSPIFALVAVLSVAIGSGATTAIVTLF
                     DTMLLRPPPGVGHAERLVTVGRTQDGRGFDNFSFPTFQAYREARSLSGLAALDIGPQA
                     VSLAGPAGGEPIHLSAASANLFDILEARPALGRFFVPDDDVVPGANPVVVLSHDFWKR
                     RFGADPSILGRPLVLNGTPFTVVGVAARGFRGPFALAPDVWVPLAATTHLGTPAGMFT
                     LRAGSWLMAIGRLAPSAGLGQARAELATIAARLAQDYPDAYDGQGVAVERATLFPGDI
                     GSAIGGFLSLLFAIAGLVLIIASTNVAGMLLARAAARRREIAVRLALGASRAQLVGQL
                     VMECLLLFGAAGVAGLVIAHWLVVGALALVPPLPMPLALDPHLDWRVLGFAFVVSLVT
                     GLLAGVVPALQSTRPDLVPALKAGASSGAGGPRLRLRSGLLVAQIALSMVLLMVAGLF
                     ARTLAHARAIDPGFEPRDVYVASLDLGLAKYDETQGRRVAGAILDQVRAVPGVRAAAL
                     AAMLPLGGGGLGLGNITVAGREPPDTRRGWDADWNVVTPGYFAALGIPLVRGRDFAPT
                     DRAGSGDVAILNEAFAAALFPGEDPVGRSFTNEGRSVTVIGVARNAKYRSVVEAQRNF
                     VYVPLSQQYLGRTNLLIKVSPGAMAPGAAVRGIVSGVDPALPILRQEALSEETDGALF
                     PQRIALLVSGGLGVVALLLALLGIYGVTAYGVVQRTREIGVRVALGAQRSHVVKLVLR
                     QGLVLAGIGVTAGALVALAATRLIRGLLYGVASTDVVALGGAAALLALAALVASWIPA
                     RRAARLDPVIALRSE"
     gene            complement(3994..>4319)
                     /locus_tag="AC16JC_05005"
     CDS             complement(3994..>4319)
                     /locus_tag="AC16JC_05005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164090.1"
                     /translation="PEGARYTGAALRAMLAVLLGDTADARSRVAALLHELPRAGPLEM
                     WTGSGLATALWQLGDRAGARAVLERMPRGAMTWALNLATLRQLDLTDPAQRRLYEENR
                     PPWAQ"
BASE COUNT          546 a         1549 c         1548 g          676 t
ORIGIN      
        1 ccccgggcga tctcgagcgc gacctcgtgc tgcagcgtca ccacgtcggg cgccggccgg
       61 gagaaggtcc gggcccagag gctctccccg gtgcgcgggt cggtcagctc cgccgacacc
      121 tgcacgacgt cccccgcctt gagcaccgcg cccgtgacga tcgcgtccac gccgagggca
      181 cgggcgatct cgctcgccga ctgcgtcgta ttgcggtacg cggctacggc acggtgcccg
      241 aggacacgca cgccggcatc ggtcagcacg ccgatcacct cgcgggtcat tccatccgcg
      301 aagtaggtct gcccggagtc gcccgtctcg ttgtccatcg gcaggacggc gatgcgctgg
      361 atcgcgccgc tgggaccggt gctccgcagc gcacgaccgc cggcccatgc aacggcgccc
      421 agggccacca cgcccaccgc gagaaccccc aaccgggcga acctgcgccg gccggcctgc
      481 gggcgcggtg acggcgcgac gttcgccgtc gagagcgcct gcacgaactg cgcggcgggg
      541 ttgaaccggt cggccgggtt cttggccagg gtgcgcgcca acgcgcccgc cacttcggcc
      601 ggcaccgtcg gccgcaggtt cgtgacggga gccgcctcgg tgaccaggtg ctggtgagtg
      661 attgcctgcg ccgtcggccc ggtgaagggc ggctgccccc ccagcatctc atacagcacg
      721 cagccgaggc tgtagagatc gctccgcccg tccaggtcgg catcgccggc ggcctgctcc
      781 ggactcatgt acatcggggt gccgacggtg atgccggtct cggtgagtcg cgcagcaccc
      841 gcggcgctca cggcccgggc gatgccgaag tcggtcacga cggcgtggcc accctcgagc
      901 aggatattct ccggcttgat gtcgcgatgc accacgcccc gggcgtgggc gtaccccaga
      961 gcgtccgcca cctcgcgcgc gatcgccagg gcctcgtcga tggggagctg cttctggcga
     1021 ttgaggcggt cgcgcagcga ctcgccctcg acgagcggca tgacgtaata gaggaagccg
     1081 tccgcctcgc ccgagtcgta cagcggcagg atgtggggat gccgcaggtt cgcggtggtc
     1141 tcgatctccc gcaggaagcg ctcgggaccc aggactgaag ccagctccga ccgcaacact
     1201 ttgatcgcca ccttgcggtg gtgtttcacg tcctcggcca gatagaccgt ggccatgccg
     1261 cccgcgccga gctcgcgctc gatgcgatag cggtcggcaa ggcttgccct gaggccctcc
     1321 accgtcattg gatcgtcacc ggagcctggt gggccgaagg ggcggttgtg aggcgggtgg
     1381 agaggtcgga cactcatggg aaagatggtc tacccgccgc actctggcga gcggcagaag
     1441 aagcagaacg tcttcatact tgggcgaaac cggctcccgt cggtggccgt aggtcccgga
     1501 ttctctccca gtcgaccgag cccatgccgg atctgttgag acagcaggtg cgctacgcgg
     1561 cccgcggcct cacgcggagc cccatcttcg ccctcgtcgc ggtgctctcg gtcgccatcg
     1621 gcagcggcgc gaccaccgcc atcgtcacgc tgttcgacac catgctactg cgcccgccgc
     1681 cgggcgtcgg ccacgccgag cgcttggtca ccgtgggacg cacgcaggat ggacgcggat
     1741 tcgacaactt ctcgtttccg acgttccagg cctatcggga ggccaggtcg ctcagcgggc
     1801 tcgcggcgct ggatatcggt ccccaggccg tcagcctcgc cgggccggcc ggcggcgagc
     1861 cgattcacct ctctgccgcg agcgccaacc tgttcgacat cctggaggcg cggccggccc
     1921 tgggacggtt cttcgtgccg gacgacgatg tcgtgcccgg cgcaaacccg gtcgtcgtcc
     1981 tgagccacga cttctggaag cggcgcttcg gggccgaccc gtcgatcctg ggccggcctc
     2041 tcgtgttgaa cgggacaccg ttcacggtgg tcggagtcgc cgcacgcggc ttccggggac
     2101 cgttcgcgct ggcgccggac gtctgggtcc cgctggcggc cacgacgcac ctggggacgc
     2161 cggcggggat gttcacgctg cgcgccgggt cgtggctcat ggccatcggc cggttggcac
     2221 cctccgccgg cctgggtcag gcgcgggccg aactggccac gatcgccgcg cgcctggcgc
     2281 aggactaccc cgacgcatac gacggacagg gcgtggccgt cgagcgagca acgctgtttc
     2341 cgggcgacat cggctcggcg atcggcgggt tcctaagcct gctgttcgcg atcgccggac
     2401 tggtgctgat catcgcgagc accaacgtcg cgggcatgct gctggcgcgc gctgccgcgc
     2461 ggcggcggga gatcgccgtg cggctcgccc tcggcgcctc acgcgcgcag ctcgtcggac
     2521 agctggtcat ggagtgtctg cttctcttcg gggctgcggg tgtcgccgga ctcgtgatcg
     2581 cacactggct cgtcgtgggc gccctcgcgc tggtgccccc tctcccgatg ccgctggcgc
     2641 tcgatcccca cctcgactgg cgggtgctcg gcttcgcctt cgtggtctcg ctcgtcaccg
     2701 gactgctcgc cggtgtcgtt cccgcgctcc agagcacccg cccggatctg gtgcccgccc
     2761 tcaaggccgg tgcctcgtcc ggtgccggcg gcccgcggct gcggctgcgg agcggtctcc
     2821 tcgtcgcgca gatcgcgctt tcgatggtgc tgctgatggt cgccgggctg ttcgcccgca
     2881 cgctcgcgca tgcccgcgcc atcgatcccg ggttcgagcc gcgcgacgtg tacgtcgcct
     2941 ccctcgacct cggattggcg aagtacgatg agacccaggg gcggcgtgtc gccggcgcca
     3001 ttctggatca ggtgcgtgcc gtgcccggag tgcgcgccgc cgcgctggcg gcgatgctcc
     3061 cgctgggcgg cggggggctc ggtctcggta acatcaccgt cgccgggcgc gagccccccg
     3121 acacacgcag gggatgggac gccgactgga acgtcgtgac ccccgggtat ttcgccgcgc
     3181 tcgggattcc attggtgcgc ggccgtgatt ttgccccgac ggaccgcgcc ggcagcggtg
     3241 acgtcgcgat cctgaacgag gccttcgccg cggcgctctt tccaggtgag gaccccgtcg
     3301 ggcgttcctt cacgaacgaa ggtcgcagcg tgacggtgat cggcgtggcg cggaacgcga
     3361 agtaccgcag cgtggtcgag gcgcaacgca acttcgtgta cgtcccgctg agccagcaat
     3421 acctcggtcg cacgaaccta ctgatcaagg tgtccccggg ggccatggcc ccgggcgccg
     3481 cggtgcgcgg catcgtctcg ggagtcgatc cggcgctccc gatcctgcgc caggaggcac
     3541 taagcgagga gacagacggc gctctgttcc cgcagcgcat cgccctgctc gtctccggcg
     3601 ggctcggcgt cgtggcgttg ctcctcgccc tgctcgggat ctacggggtg acggcgtacg
     3661 gggtcgtaca gcgaacccgg gagatcggcg tgcgggtggc cctgggcgcg cagcggtccc
     3721 acgtggtcaa gctcgtgctg cggcaggggc tggtgctggc gggcatcggc gtaacggcgg
     3781 gtgcgctcgt ggccctggcg gcgacccgcc tcatcagggg tctcctctat ggggtcgcgt
     3841 cgaccgacgt cgtcgcgctg ggtggggcgg cggcgctact cgccctcgcg gccctcgtgg
     3901 cgagctggat cccggcgcgg cgggcggcgc gcctcgaccc ggtcatcgcg ctgcggagcg
     3961 agtagcgggc ggcgggtgcg gtcgtgaggc gactcactgc gcccacggcg gccggttctc
     4021 ctcgtagaga cggcgctggg ccgggtctgt cagatcgagt tgccgcaacg ttgccaaatt
     4081 cagcgcccag gtcatggcgc cccggggcat acgctcgagg accgcgcggg cgcccgcccg
     4141 gtcgcccagc tgccacagcg ccgtcgcgag acccgagccg gtccacatct ccagcgggcc
     4201 ggcccgaggc agctcgtgca gcagcgccgc gacacggctg cgggcgtcgg ccgtatcccc
     4261 gagcagaacc gccagcatcg cccggagcgc ggcgcccgta taccgtgccc cttccggtg
//
LOCUS       DBNGZW010000207         1334 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1587833, whole genome shotgun sequence.
ACCESSION   DBNGZW010000207 DBNGZW010000000
VERSION     DBNGZW010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1334)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1587833"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="AC16JC_05010"
     CDS             <1..1126
                     /locus_tag="AC16JC_05010"
                     /inference="COORDINATES: protein motif:HMM:NF036531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HIR5164091.1"
                     /translation="LLQRAAQLSWEDRFLMAEMLARRRELTAARALLIPAWAQLKLEG
                     RRAVLPSDVSAKYFYFWSTIRPAARLLTATLAVDPQNPFVGPLVETVAQRGHAEQFDW
                     WWDTQDYASAVEALTRYDTYRRAAAERPVRVSRGAHVLFEKPSGGTFSDTTIALTGLL
                     ATRPDGKQALQVSLLAPGTGAPVFYYLTVREVPQGRPVRPSDAGIRVERWYERYDAPT
                     PITRAVEGDLVRVKLRVTIPALRNFIVVDDALPAGLEAVDISLRGAGLPPVAQQQNRE
                     ENPDEEEQQVAGAEGAGWYYGYWEYGWWSPWDHRELRDDRVVYVATTLWPGTYTMTYV
                     ARATTPGMFVRPPVYAEEMYNPGVNGRSDGGVFTVTAKGP"
BASE COUNT          206 a          438 c          482 g          208 t
ORIGIN      
        1 tctgctccag cgggccgccc agctctcatg ggaggaccgc ttcctcatgg cggagatgct
       61 ggcgcgacgc cgtgagctga cggccgcccg cgcgctgctc atcccggcgt gggcgcagct
      121 gaagctcgaa gggcgtcgcg ccgtcctgcc gtccgacgtc tcggccaagt acttctattt
      181 ctggtccacc atccggccgg cggcccggtt gctcacggcg acgctcgccg tcgatccgca
      241 gaaccccttc gtgggcccgc tggtcgagac cgtcgcccaa cgcggccacg ccgagcaatt
      301 cgactggtgg tgggacacgc aggactacgc ctcggccgtc gaggcgctca cccgctacga
      361 cacgtaccgc cgggcggccg ccgagcgacc ggtgcgggtg agccgcggtg cccacgtgct
      421 gttcgagaaa ccgtccggcg gcacgttcag cgacacgacg atcgcgctca ccggactgct
      481 cgccacccgg cccgacggca agcaggccct gcaggtctcg ctgctggcgc cgggaactgg
      541 tgcgccggtc ttctattacc tgacggtgcg cgaggtcccg cagggacgcc cggtgagacc
      601 ctcggacgcc gggatccggg tggaacgctg gtacgagcgc tacgatgcgc ccacacccat
      661 cacccgcgcc gtcgagggag acctggtgcg agtcaagctg cgcgtgacga tccccgcgct
      721 gcgcaacttc atcgtggtgg acgacgcgct gcccgccggc ctcgaggcgg tggacatctc
      781 gttgcggggc gcaggcctgc ccccggtggc gcagcagcag aaccgggagg aaaaccccga
      841 cgaggaggag cagcaggtgg ccggcgccga gggagcgggc tggtactacg ggtactggga
      901 gtacggctgg tggtcgccgt gggaccaccg ggagctgcgc gacgatcgcg tggtgtacgt
      961 cgccaccaca ctctggcccg ggacctacac catgacctac gtggcgcgcg ccacgacgcc
     1021 cggcatgttc gtgcggccgc ccgtgtacgc ggaggagatg tacaatccgg gggtgaacgg
     1081 gcgcagcgac ggcggcgtgt tcacggtgac ggccaagggg ccgtgagggg ccgatgtatc
     1141 gtcttttcca gggaggcaac gatgacactg gacatgctgg tggtctgggt tgtggttgga
     1201 ggggttgccg gtctgctggc agatgctctg atcaagaaca tcaaggtcgg aatcgtcgga
     1261 gccatcctca tcggaatact cggcgcattc atcggtggct ggctgttcag gcagttgggg
     1321 atacgcgtgg gaac
//
LOCUS       DBNGZW010000208         3033 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1717162, whole genome shotgun sequence.
ACCESSION   DBNGZW010000208 DBNGZW010000000
VERSION     DBNGZW010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1717162"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..713)
                     /locus_tag="AC16JC_05015"
     CDS             complement(<1..713)
                     /locus_tag="AC16JC_05015"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR5164092.1"
                     /translation="MPHLTRRGFTLVELLIALLLMGIVTAAFYGSLTTNQRAYQKQTQ
                     VMDLQQNMRAATAILPSELREIDATEGDISAMSATSITFRAHRWMGFICTPPVMPVVA
                     NPITMILRRDPFYGRTPINTATDVMFVRYEGDDGTRRDDGWAQAQPTAVLNGVCPDTK
                     PGLVVTMNLTLGALPNIPEGITSGAPVLGYETVTYGLYKPSGSVDWYIGLTTANGQQP
                     LIGPLLPNGLSLTYYDSTGA"
     gene            complement(701..1081)
                     /locus_tag="AC16JC_05020"
     CDS             complement(701..1081)
                     /locus_tag="AC16JC_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR5164093.1"
                     /translation="MTRHNQGGFTILEVMIALVILVVGMLGLVMTSALLSRMMGRSSH
                     AQAASTFAAQRMERMRPAACIAAQRVAGEDTLMRGGRWVAINQWSYTDLGGNAYRLQV
                     ITRWQTTNNRTRTDTLEMGVPCLT"
     gene            complement(1078..1524)
                     /locus_tag="AC16JC_05025"
     CDS             complement(1078..1524)
                     /locus_tag="AC16JC_05025"
                     /inference="COORDINATES: protein motif:HMM:NF023444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HIR5164094.1"
                     /translation="MGNRGFSFVEMLVVFIIVGIVVAMGMPRLRDSLEKSNVRSAKAG
                     MATLVAKARAAAVSRGCTATMSVTSGTVAISVCTVGGGGTQILGGVDSLAARYKVTMT
                     ASAPSMQFDPRGLSVNYSGMTVRFSNTSGSLSDSVIINPLGKVVRQ"
     gene            complement(1657..3003)
                     /locus_tag="AC16JC_05030"
     CDS             complement(1657..3003)
                     /locus_tag="AC16JC_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5164095.1"
                     /translation="MSRVLVIDDEPGLRQSLGLLLGDAGYSVAAEADGRRGLERALAE
                     PFDLVLCDVRMPQLSGIEFLRAYRDRGGTALVIMMSAYGGEDAALAAMKEGAYDYVPK
                     PFRPDEVVLALRKAEERERLRRTVAGLRAQLDSGPDARALVVASPAMQAAMDVVARVA
                     EHKTTVLITGESGTGKEVIARAIHRASPRAGAAFIGINCAAIPENLLESELFGHVRGA
                     FTGASSEKTGLFEQANGGTLLLDEIGDLPLGLQVKLLRVLQEGEVRRVGDTRDRPIDV
                     RVLTATERDLPADVRGGRFREDLFYRLHVVAIHLPPLRARREDVAPLARLFAARLGSR
                     FGFPVTLSEGAIAWLEAQPWPGNVRELENAIERAAVLSGKRELDPGDFDARPLAPPDT
                     AVAAGKVTLPDAVAEAERTAIRTALEASGGNRRAAAKRLGVSLRTLFYKIQRYGLE"
BASE COUNT          478 a         1063 c         1013 g          479 t
ORIGIN      
        1 gcgcccgtcg agtcgtagta ggtgagcgag agaccgttgg gcagcagcgg accgatgagc
       61 ggctgctggc cgttggccgt cgtgagaccg atgtaccagt ccacgctgcc gctcggcttg
      121 tacaggccgt aggtcaccgt ctcgtagccg agcaccggag cgccactggt gatcccttcg
      181 gggatattcg gcagcgcgcc cagggtcagg ttcatggtca ccaccagtcc cggcttggtg
      241 tcggggcaga caccgttgag caccgcggtc ggttgcgcct gcgcccagcc gtcgtcccgg
      301 cgcgtgccgt cgtctccctc gtagcgcacg aacatcacgt ccgtcgcggt attgatgggc
      361 gtgcgcccgt agaacgggtc gcggcggagg atcatcgtga tggggttggc caccaccggc
      421 atcaccggcg gcgtgcagat gaatcccatc cagcgatgcg ctcggaacgt gatcgacgtc
      481 gccgacatgg cgctgatatc gccctcggtg gcgtcgatct cccgcagctc cgacggcaga
      541 atcgcggtcg ccgcgcgcat gttctgctgc aggtccatca cctgcgtctg cttctggtag
      601 gcgcgctgat tggtcgtcag ggagccgtag aaggccgccg tgacgatgcc catgagcagg
      661 agcgcgatca gcagctcgac cagggtgaaa ccgcgccgcg tcaggtgagg catggaaccc
      721 ccatctccag cgtgtcggtc cgcgtgcgat tgttcgtcgt ctgccacctg gtgatgacct
      781 ggaggcggta cgcgttgccg ccgaggtcgg tatagctcca ctgattgatc gcgacccacc
      841 ggccgccgcg catcagcgtg tcttccccgg ccactcgctg ggccgcgata cacgccgccg
      901 gccgcatccg ctccatgcgc tgcgccgcga acgtcgaagc tgcctgggcg tggctcgacc
      961 ggcccatcat ccgcgacagc agcgcggagg tcatcaccag gccgagcatg cccacgacca
     1021 ggatgacgag agcgatcatc acctcgagga tggtgaatcc gccctggttg tgccgggtca
     1081 ttgccgcacc accttgccca acgggttgat gatcacggaa tcggagaggc tgccggaggt
     1141 gttgctgaat cgcacggtca tgcccgagta gttcacgctc agcccgcgag gatcgaactg
     1201 catcgagggg gcgctcgcgg tcatggtgac cttgtagcgg gccgccaggg agtcgacgcc
     1261 gcccaggatc tgggtgccgc cgccgcccac ggtgcagacg gagatggcca ccgtcccgga
     1321 ggtgacgctc atcgtggccg tgcatccgcg ggacaccgcg gcggcccggg ctttcgccac
     1381 cagggtcgcc atcccggcct tggcactgcg cacgttcgac ttctcgagcg agtcccgcag
     1441 tcgcggcatg cccatcgcga ccacgatccc gacgatgatg aagacgacca gcatctcgac
     1501 gaatgagaag ccacgattgc ccatggtgcg ttcctccatg cgggcggcac catacacact
     1561 ccgtgcctca gcgtaacaca ttgaaaacag ttgtcttacg gaatgcggtc gagtcccggc
     1621 gagtgcaaat acccgcgtcg cgtgcaaaat actgcactac tcgaggccgt agcgttggat
     1681 cttgtagaac agcgtgcgga gactcacgcc cagccgtttg gccgccgccc ggcggttccc
     1741 cccgctcgct tccagcgcgg tccgaatcgc cgtccgctcc gcctcggcca cggcgtcggg
     1801 cagggtcacc ttcccggccg caacggccgt gtcgggtggc gcgagcggcc gcgcgtcgaa
     1861 gtccccgggg tcgagctccc gcttgccgga caacacggcc gcgcgctcga tcgcgttctc
     1921 cagctcgcgc acgttcccgg gccacggctg cgcctcgagc caggcgatcg ccccctcgga
     1981 cagcgtgacg ggaaagccga agcgcgagcc cagccgtgcg gcgaagagcc gggccagggg
     2041 cgccacatcc tcgcgccggg cgcgcagcgg cggcagatgg atggcgacga cgtgcaaccg
     2101 gtagaacaga tcctcgcgga accgcccgcc gcggacgtct gcggggaggt cgcgctcggt
     2161 cgccgtcagc acgcgcacgt cgatcgggcg gtcgcgagtg tcccccacgc ggcggacttc
     2221 tccttcctgt aacacgcgca gcagtttcac ctggagaccg aggggaaggt caccgatctc
     2281 gtccagcaac agggtgccgc cgttggcctg ctcgaacaga ccggtcttct cgctcgacgc
     2341 cccggtgaac gcgccgcgca cgtggccgaa cagctccgat tcgagcaggt tctccggaat
     2401 cgccgcgcag ttgatcccga tgaatgccgc gcccgcgcgc gggctggccc ggtgaatggc
     2461 gcgggcgatg acttccttgc cggtgccgct ttcgcccgtg atcagcaccg tcgtcttgtg
     2521 ctcggcgacc cgcgccacca cgtccatggc ggcctgcatg gcgggactcg ccacgaccag
     2581 ggcacgggcg tccgggccgc tgtcgagctg cgcgcgcagc cccgcaaccg tgcgccgcag
     2641 gcgctcccgc tcctcggcct tgcgcagcgc caggaccacc tcgtcggggc ggaacggctt
     2701 ggggacgtag tcgtacgccc cttccttcat cgcggccaac gccgcatcct cgccgccgta
     2761 ggcactcatc atgatgacca gcgccgtgcc tccgcggtcg cgataggccc gcagaaactc
     2821 gatgccggag agctgcggca tccgcacgtc acacagcacc agatcgaacg gctcggcgag
     2881 cgcgcgctcg aggccgcggc gcccgtcggc ttcggccgcg acgctgtacc cggcgtcgcc
     2941 gagcagcaag cccagcgact gccgcagacc gggctcatcg tcgatgacca gaacgcggct
     3001 catatgaaag acattgcgga ttggggattg ggg
//
LOCUS       DBNGZW010000209         7402 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1035426, whole genome shotgun sequence.
ACCESSION   DBNGZW010000209 DBNGZW010000000
VERSION     DBNGZW010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7402)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7402)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7402
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1035426"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1309)
                     /locus_tag="AC16JC_05035"
     CDS             complement(<1..1309)
                     /locus_tag="AC16JC_05035"
                     /inference="COORDINATES: protein motif:HMM:NF020037.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR5164096.1"
                     /translation="MIPGTRVTRTVLVAAGGLLAVLVLAYLFLPVPRSLLPAGSWTLT
                     ALARTAGIALAWRAATDPVQRRGWAWLLAGSVVDFATQGFADIQLARGIVRPGMTAVD
                     IGYLAVYPCFFIAAWTLLAIQPRRRLTAEILLDSALLTLTAVAWAYDLFVEPLTGGVG
                     GREVLGSVAYATGGLALVWLIGVLLLQPNPIPRGGAELAVVGLLLYGIADLLFGTVSL
                     PRAEAPGTLLGFGWNVANLVLAAAGVVAAASPAGARPAAAGTAAVAPRLLAVLLGLAG
                     LVALHIHNMASGMVGTDDVLVPAVGGMIIAARVAYSLVADRRYEAALEREVERQTQTL
                     VDSLTTTASAERNLRQIMEASPDPIVLLNRDGQVLDYAPDRPAHGAAAGTRTAFDFVD
                     PDAVALLRAHLEAAFAGEVRRFEVPQRRPDGARVVSSVVYAPIR"
     gene            1393..2706
                     /locus_tag="AC16JC_05040"
     CDS             1393..2706
                     /locus_tag="AC16JC_05040"
                     /inference="COORDINATES: protein motif:HMM:NF012562.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HIR5164097.1"
                     /translation="MEDRLGWTRAVPDSLERWPEWRRFAAAVRRDGLDRVLICGMGGS
                     ALAPAVLARSFRTGGLDVLDSTDPAAVAAAERMHPLDRTLFLIVSKSGSTVETLAAYH
                     YFAARARPGQFVAVTDPGSALERLARERGFRAVFPHPADVGGRYAALTVVGMLPAALA
                     HIDGAALLERARATDVAAAQAFGRSLAAAAAAGRDKLALEPPAPVAALASWIEQLVAE
                     SSGKNGAGIVPLVGGSDGGGDDVHVVHEFSADPLELGREFLRWEYATWELCRGLGVNA
                     FDQPDVEQAKALARAELAAPAGSGPAVPHTLSLDELRRAARKGDYLAILAYLPPRPDL
                     EQALVRVRRAWGSALGVVTTQGFGPRYLHSTGQLHKGGPNTGLFLVITSDPAQDMEIP
                     TMQTTFGRRERAQAIGDVRALLARGRRVCYHHLRRPEDVAELERV"
     gene            2703..5333
                     /locus_tag="AC16JC_05045"
     CDS             2703..5333
                     /locus_tag="AC16JC_05045"
                     /inference="COORDINATES: protein motif:HMM:NF013256.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 N-terminal
                     domain-containing protein"
                     /protein_id="HIR5164098.1"
                     /translation="MITFHLIIHTHWDREWHLPGATYQARLVPMMDDLLQRLEADAGF
                     RSFLLDGQTVLLEDYLRARPERRETVQQLVRAGRLQVGPWYVLADEIVPAGESLVRNL
                     LLGAADAARLGGRLDVLYSPDAFGHPAWWPELARGAGCACGVLWRGLGGVPGQERDRY
                     RWRGPSGAEVRLWHLPPTGYEIGVELAADSEALPELWAWVRGELVGRAGGSQIPVFIG
                     ADHHEAPADVSRLREQLAELERPNAVRVSRLDEFFAAAAAEFDAAPVLTGSLRSAPGY
                     AWTLQGAQGTRAPLKRRAALLELWLARVAEPLAALAARQRAHDQRPVLELAWRALVQS
                     QFHDTIAGTTSDAVARATDQRLDAVHAYADEVIRSAAFALVGHDPDRTRERPDLQAPA
                     LVLWNPAPHARGGVVIADCTFFRRDVPVGPPGPRKPRAGPGAQAFSLRAARGHAVPLQ
                     VLDRRRGQERLDASRHYPDQDEVDVVRVAFRAPLLPGFGLATLSCGAPATSPSEDTVQ
                     VQARSLVNRFVVATLDRAGSLQIYDRRTGSRWLDLLRLESEADAGDAYSFCAAARDRL
                     VRSQGPIQVRRVAAGPLVAALEARWTLRAGGGRRGKVGVRLLVSLHADSPVVRCIFDV
                     DNGALHHRLRARVPMGSVEPALVGTQFGQEVRPLTPPGSARHALETPVATAPAHRFVA
                     VVSGGQGLAALAPGFFEYEHTPSGDIVLTLLRAIGALSRGDLPVRPGHAAWPTAIPEA
                     QCLGVSRVELALAPLAAGESIPACWESVFTPVRGLWIRDAISGGATPHRAGITLDGAG
                     LVFSALKPADGDPRDVVLRCYNPDDRPVAGAWRFAAPVSAAFRTRLDEGEPVPLVLED
                     GGRVVRFTAGPRDIVTVMVR"
     gene            5336..5986
                     /locus_tag="AC16JC_05050"
     CDS             5336..5986
                     /locus_tag="AC16JC_05050"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5164099.1"
                     /translation="MEEHHFTTSRTSRHYTLGPAAAAAREVWFLLHGYGQLAGRFLGA
                     FQSLDDGTRCLVAPEALSRFYLGETGGRHDKVGASWMTREDRLTDIADYVRYLDALHG
                     EILRGRGDAGGVRVLGFSQGTATAARWLALGAARADRLILYGGEMPPDLDLAAARERW
                     GRTELILAAGSHDSYITPKILARDEDRLRAHGIEYRVERFDGGHEVLPGVLRRIAE"
     gene            complement(6004..6195)
                     /locus_tag="AC16JC_05055"
     CDS             complement(6004..6195)
                     /locus_tag="AC16JC_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164100.1"
                     /translation="MQPPTASWGNMIASGRDTLVNAPWVAAAPGGMLVLVVIGCTLVG
                     DALRDVLSRQSSAISRQGL"
     gene            complement(6208..6864)
                     /locus_tag="AC16JC_05060"
     CDS             complement(6208..6864)
                     /locus_tag="AC16JC_05060"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5164101.1"
                     /translation="MNTALQDRRLTVGLTLLAGVAALALAAPIVTGGDPNAPGDIVAT
                     RFLGLLSRDVHGTVHLLGTDRFGRDVWTRLVYGARISLAVGFLSVFLSVAVGVAIGGL
                     SGWWRGWPSTLLLGCTDFALALPRVVLLLLLAALWQPSALLVVLLLAATGWMPIARLV
                     HGEVRALAVRPFVEGARSLGVRPLRVLWRHVLPNALTPVIVAAALGVGNAILLDAGLS
                     "
     gene            complement(6861..>7402)
                     /locus_tag="AC16JC_05065"
     CDS             complement(6861..>7402)
                     /locus_tag="AC16JC_05065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5164102.1"
                     /translation="LMLVTVFTYLLRWLPAFGAAGYDADYLGGWGRLLDGLRHLALPL
                     ATLTLIGIGAAARFVRGALLDALEQPFVVTARAKGLRDSAVMVHHALRHALIPVITLL
                     GLSLPALFSGAVFVEAVFAWPGVGRVLVDAVHARDYPVVMAATAISALLVVAGNLLAD
                     VAAGLVDPRLRAAEAAPPQ"
BASE COUNT         1042 a         2640 c         2746 g          974 t
ORIGIN      
        1 cgcggatcgg ggcatacacc accgagctca ccacgcgcgc cccgtcgggc cgccgttgcg
       61 gcacttcgaa gcggcgcacc tcccccgcga acgccgcctc gaggtgggcg cgcagcagcg
      121 cgaccgcgtc cgggtccacg aagtcgaacg cggtgcgggt accggcggcc gcgccgtgcg
      181 ccggtctgtc gggggcgtag tccaggacct ggccgtcccg gttcagcagc acgatcgggt
      241 ctggcgacgc ctccatgatc tggcggaggt tgcgctccgc cgacgccgta gtcgtcaacg
      301 aatcgacgag ggtctgcgtc tgccgctcga cttcgcgctc caacgcggcc tcgtaacgcc
      361 ggtcggccac cagggagtac gcgacgcggg cggcgatgat catcccgccg acggcgggga
      421 ccagcacgtc gtccgtcccc accatgccgg aggccatgtt gtggatgtgg agcgccacca
      481 gcccggcgag ccccaacagc acggccagca atcgtggcgc gacggcggcg gtacccgcgg
      541 ccgccggccg cgcccccgcg ggagacgcgg cggcgacgac tcccgccgcg gccagcacca
      601 ggttggccac gttccagccg aagccgagca gggtgcccgg ggcctccgcc cgaggcaggc
      661 tcacggtccc gaacagcaga tccgcgatgc cgtagagcag cagcccgacc accgcgagct
      721 ccgcgccgcc acgaggtatc ggattcggct gcagcagcag cacgccgatc aaccagacga
      781 gggcgagtcc gccggtggcg tacgcgacgc tgcccaggac ctcccgcccg ccgacgcccc
      841 cggtgagcgg ctccacgaac aggtcgtacg cccaggcgac ggcggtgagg gtgagcaacg
      901 cggagtcgag cagaatctcc gccgtgagcc gccggcgcgg ctggatggcg agcagggtcc
      961 aggcggcgat gaagaagcac gggtatacgg ccaggtatcc gatgtcgacg gcggtcatgc
     1021 ccgggcggac gatgccccgc gccagctgga tgtcggcgaa gccctgtgtg gcgaaatcga
     1081 cgacgctccc ggccagtaac cacgcccacc cgcgccgttg gaccggatcg gtggccgcgc
     1141 gccacgccag ggcgatcccc gccgtgcgcg ccagcgccgt cagcgtccag ctgcccgccg
     1201 gcagcaggct ccggggcaca gggaggaaga ggtaggccag gaccagaaca gccaacaggc
     1261 cgccggctgc cacgaggacg gtgcgggtga cgcgggtgcc tgggatcacg cgaatagcca
     1321 gaaagtagtg acgggcgcat ccgcaggcga gggcgcgcga cgcggcagtg cccgcgttag
     1381 cttagcgccg gcatggaaga tcgactcggt tggacccggg ccgtacccga ctcgctcgag
     1441 cgatggccgg aatggaggcg cttcgccgcc gcggtgcggc gcgacggcct cgaccgggtg
     1501 ctcatctgcg ggatgggcgg gtcggcgctc gctcctgcgg tgctcgcacg cagcttccgg
     1561 acgggggggc tggacgtgct ggacagcacc gaccccgcgg ccgtcgcggc ggcggagcgg
     1621 atgcacccgc tggaccgcac gctctttctg atcgtcagca agtcgggctc gacggtcgaa
     1681 accctcgctg cctatcacta cttcgccgcg cgggcgcgcc ccgggcagtt cgtggcggtc
     1741 accgatcccg gcagtgcgct cgagcggttg gcgcgcgagc ggggattccg ggccgtgttc
     1801 ccacaccccg cggacgtggg cggtcgctat gccgcgctca cggtggtggg aatgctgccg
     1861 gccgcactcg cgcacatcga tggagcggcg ctgctcgagc gcgcccgggc aaccgacgtc
     1921 gcggcggcgc aggcgttcgg gcgctcgctc gccgccgccg ccgccgcggg ccgcgacaag
     1981 ctggccctcg agccgccggc ccccgtcgcg gcgctggcga gctggatcga gcagctcgtc
     2041 gccgaaagct cggggaagaa cggggccgga atcgtcccgc tggtcggggg ctccgacggc
     2101 ggcggcgacg acgtccacgt ggtgcacgag ttttccgccg atcccctcga gctcggccgg
     2161 gagtttctgc gctgggagta cgccacctgg gagctgtgcc gcggcctcgg cgtcaacgcc
     2221 ttcgatcagc ccgacgtcga gcaagccaag gcgctggccc gcgccgagct ggccgcgccc
     2281 gcgggttcgg gcccggcggt gccgcacacc ctgagcctcg acgagctgcg ccgggccgca
     2341 cgcaagggtg actacctggc gatcctcgcc tacctgccgc cccgtcccga tctggagcag
     2401 gcgctcgtgc gtgtgcgccg ggcgtggggg agcgcgctcg gcgtggtgac cacgcagggt
     2461 ttcggaccgc gctacctcca ctccaccggc cagctgcaca agggcggacc gaacaccgga
     2521 ctgtttctcg tgatcacgtc ggaccccgcc caggacatgg aaattcccac gatgcagacg
     2581 accttcggcc ggcgcgagcg cgcgcaggcg atcggcgacg ttcgcgccct gctcgcgcgg
     2641 ggccgccgtg tctgctacca ccatctgcgc cggccggaag acgtggccga gctcgagcgg
     2701 gtgtgatcac cttccatctc atcatccata cgcactggga ccgtgagtgg caccttcccg
     2761 gcgctactta tcaggcgcgg ctcgttccca tgatggatga tctgctgcag cgtctcgagg
     2821 ccgatgcggg attccgcagc ttcctgctgg acggccagac cgtcctgctc gaggactacc
     2881 tgcgcgcccg gccggagcgg cgtgagacag tgcagcagct ggtgcgggcg ggacgcctgc
     2941 aggtgggacc atggtacgtg ctcgccgacg agatcgtgcc cgccggcgag tcgctcgtgc
     3001 gcaacctgct gctcggggca gccgacgccg cgcgattggg cgggcggctc gacgtgctct
     3061 attcgccgga cgccttcggg catcccgcct ggtggcccga gctggcccgc ggagcgggct
     3121 gcgcctgcgg cgtcctgtgg cgcgggttgg gtggtgtgcc cgggcaggag cgggaccgct
     3181 atcggtggcg cggcccgagc ggtgccgaag tccggttgtg gcacctcccg ccgacggggt
     3241 atgagatcgg cgtggagctg gcggcggact cggaggcgct gccggagctg tgggcctggg
     3301 tacgcggcga gctggtgggt cgagccgggg ggagccagat cccggtgttc atcggagccg
     3361 atcatcatga ggcgccggcc gatgtgtcgc gcctccgcga gcagctcgcc gagctggagc
     3421 ggcccaacgc ggtgcgcgtg tcgcggctcg acgaattctt cgccgcggcc gccgccgaat
     3481 tcgacgcggc acccgtcctg accgggtcgt tgcgctccgc tcccggatat gcctggacgc
     3541 tgcagggcgc gcagggcacc cgcgccccgc tcaagcggcg ggcggcgctg ctcgagttgt
     3601 ggctggcgcg cgtggccgaa cccctcgcgg cgctggcggc ccggcagcga gcgcacgatc
     3661 agcgaccggt gctcgagctg gcctggcgcg ccctggtgca gtcgcaattc cacgacacga
     3721 ttgccggcac gacgagcgac gcggtggcgc gcgcgaccga ccagcggctc gacgcggtgc
     3781 acgcgtacgc ggacgaggtg attcgatccg ccgcgttcgc gctcgtgggg cacgacccgg
     3841 accgcacccg cgagcggccg gatcttcagg cgcccgcgct cgtgctctgg aatccggcac
     3901 cgcacgcgcg gggaggcgtc gtgatcgccg actgcacgtt cttccgtcgc gacgttccgg
     3961 tcggaccgcc cggaccgcgc aagccgcgcg ccggtcccgg ggcgcaggcg ttctcgttgc
     4021 gcgccgcccg tggtcacgcc gttccgctcc aggtgctcga ccgccggcgc gggcaggagc
     4081 ggctcgacgc gagccggcac tacccggacc aggacgaggt ggacgtggtg cgggtcgcgt
     4141 tccgggcgcc gctcctcccg gggttcggcc tggccacgct gtcctgcggg gcgccggcca
     4201 cgtcgccgtc cgaagacacg gtgcaggtcc aggcgcgctc cctcgtcaac cgttttgtgg
     4261 tggcgacgct cgaccgggcc ggcagcctgc agatctatga tcgccgcacc ggctcgcgct
     4321 ggctcgatct gctgcgtctc gaaagcgagg ccgacgccgg ggatgcctac agcttttgcg
     4381 cggccgcgcg cgatcgcctc gtgcgcagcc aggggccgat ccaggtgcgg cgtgtcgcgg
     4441 cggggccgct ggtcgcggcg ctcgaggccc ggtggacgct gcgcgccggc ggcggacggc
     4501 gggggaaggt cggcgtgagg ctgctggtct cgctccacgc cgacagcccg gtcgtgcggt
     4561 gcatattcga cgtggacaac ggcgcgctgc atcaccggct gcgcgcccgg gtgccgatgg
     4621 gatcggtcga gccggccttg gtcggcacgc aattcggaca ggaagtgcgg ccgctcacac
     4681 ccccgggctc ggcacgccac gcgctcgaaa cgcccgtggc cacggcgccg gcgcatcgct
     4741 tcgtcgcggt cgtgagcggc gggcaggggc tggccgcgct cgctccggga ttcttcgagt
     4801 acgagcacac gccgtccggg gacatcgtcc tcaccctgct gcgcgccatc ggcgcgctct
     4861 cgcgcggcga cctgccggtt cgccccgggc atgcggcctg gcccacggcg attcccgaag
     4921 ctcagtgtct cggggtctcc cgggtcgagc tggcgttggc gccgctcgct gccggggaat
     4981 cgattccggc ctgctgggag agcgtcttca ctcccgttcg gggactctgg atccgcgatg
     5041 cgatcagcgg gggagccacg ccgcaccgcg cgggcatcac gctggacggc gcggggctcg
     5101 tattctccgc gctgaagccg gcggacggcg atccacggga cgtcgtgctc cgctgctaca
     5161 atcccgacga ccgaccggtc gctggcgcgt ggcgcttcgc ggcgcccgtg agcgccgcgt
     5221 ttcgcacccg gctcgacgag ggggaaccgg tgccgctcgt gctggaagac ggtggccgtg
     5281 tggtgcggtt caccgccgga ccgcgcgaca tcgtgaccgt gatggtgcgt tgaccgtgga
     5341 ggagcatcac ttcacgacgt cccggacctc ccgccactac acgctgggtc ccgccgctgc
     5401 ggcggcgcgc gaggtctggt tcctgctcca cggctacggg cagctggccg ggcggttcct
     5461 gggggcattc cagtctctgg atgacggcac ccggtgcctc gtcgcgcccg aggcgctgtc
     5521 gcggttttat ctcggcgaaa cgggcgggcg gcacgacaag gtgggcgcga gctggatgac
     5581 gcgcgaggac cgtctcaccg atatcgccga ttacgtgcgc tatctcgacg cactgcacgg
     5641 cgagatcctg cgcgggcgtg gagacgccgg cggagtgcgc gtgctcgggt tctctcaggg
     5701 caccgcgacg gcggcgcgct ggctggcgct gggggccgcg cgcgccgacc gattgatcct
     5761 ctacggcggc gagatgccgc ccgacctcga tcttgccgcg gcacgggagc gctggggacg
     5821 cacggagctg atcctcgcgg cgggcagtca cgattcctac atcaccccca agatcctggc
     5881 gcgcgacgag gatcgcctgc gcgcacacgg catcgaatac cgcgtggaac ggttcgatgg
     5941 cgggcacgag gtgctgcccg gggtgcttcg gcggattgcg gaatagtggt cggtggccgg
     6001 ttgtcagagc ccctgacgac tgatggctga cgactgacgg ctcagcacgt ctctcaacgc
     6061 atctcccacc agcgtacacc cgatcaccac cagtaccagc atcccacccg gcgccgccgc
     6121 aacccagggc gcgttgacca gggtgtcgcg tcccgaggcg atcatgttgc cccaggaggc
     6181 cgtgggcggc tgcacgccga gcccgagtta ggagagcccg gcatcgagca ggatcgcgtt
     6241 gccgaccccg agcgcggcgg cgacgatcac cggcgtcagg gcgttgggga gcacgtgacg
     6301 ccacagcacc cgcagcgggc gcacgcccag gctccgggcg ccctctacga aaggccgcac
     6361 cgccagggcg cgcacctcgc cgtgcacgag ccgggcgatc ggcatccatc cggtggcggc
     6421 cagcagcagc acgacgagca gcgcgctggg ttgccacagc gccgccagca gcagcagcag
     6481 gacgacgcgc ggcagcgcca gggcgaaatc ggtgcagccc aggagcagcg tgctcggcca
     6541 gccgcgccac cagcccgaga gcccgccgat cgccacgccg accgcgaccg acaagaagac
     6601 cgacaggaat cccaccgcga gggagatccg ggcgccgtac acgagccgcg tccacacgtc
     6661 gcggccgaag cggtcggtgc cgagcaggtg cacggtgccg tgcacgtcgc gcgacaggag
     6721 gccgaggaag cgggtcgcga cgatgtcgcc gggcgcgttg gggtcgccgc ccgtgacgat
     6781 gggcgccgcc agagccaggg cggccacacc ggcgagcagg gtgagtccga ccgtgaggcg
     6841 ccggtcctgc agggccgtgt tcactgggga ggcgccgcct cggcggcgcg caggcgcggg
     6901 tccacgagcc cggctgccac atccgcgagc aggttgccgg cgacgacgag cagggcgctg
     6961 atagcggtcg cagccatcac gacgggatag tcgcgcgcat gtaccgcatc gaccagcacc
     7021 cgtccgacgc ccggccaggc gaagaccgct tccacgaaga ccgcaccgga aaagagcgcc
     7081 ggcagggaga gccccagcag cgtgatcacg ggaatcaacg cgtgtctgag cgcgtggtgc
     7141 accatcacgg ccgagtcccg gagtcccttg gcccgtgcgg tgacgacgaa cggctgctcc
     7201 agcgcgtcga gcagggcgcc gcgcacgaag cgcgccgccg cgccgatgcc gatcagcgtc
     7261 agcgtcgcaa ggggcagcgc cagatgacgg agcccatcga gcaatcggcc ccagccgccg
     7321 aggtagtcgg cgtcgtaccc ggccgcgccg aacgccggca gccagcgcag caggtaggtg
     7381 aacaccgtca cgagcatcaa cc
//
LOCUS       DBNGZW010000210         1458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1110844, whole genome shotgun sequence.
ACCESSION   DBNGZW010000210 DBNGZW010000000
VERSION     DBNGZW010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1458
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1110844"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="AC16JC_05070"
     CDS             complement(<1..250)
                     /locus_tag="AC16JC_05070"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003008169.1"
                     /note="NADP-dependent; catalyzes the oxidative
                     decarboxylation of malate to form pyruvate; decarboxylates
                     oxaloacetate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HIR5164103.1"
                     /translation="MSSRRQEALDYHSQGRPGKIQVSPTKPFKNQRDLSLAYTPGVAE
                     PCLEIARRPEDVYLYTTRGNLVAVVSNGTAVLGLGDIGA"
     gene            complement(347..991)
                     /locus_tag="AC16JC_05075"
     CDS             complement(347..991)
                     /locus_tag="AC16JC_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164104.1"
                     /translation="MRGGRWAVLGTVLAAAGCTDPLVPSRSAIYPFADAFGDVFHWPA
                     ERLPVHIYADPRGDLSALVSRAVDLWARQLLYGELTVVMVADSNRADVIVRWADSVPP
                     AATPDPGPPVEACGGVTQGVLDGTGQAFAAPFETEISILTGTVFTPEQVAACVRRTTL
                     HELGHSFGLFQETADTLSAMRSPPLVAEPAAIDRRTVQVLYHTPATLAPPPRRP"
     gene            complement(988..>1458)
                     /locus_tag="AC16JC_05080"
     CDS             complement(988..>1458)
                     /locus_tag="AC16JC_05080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164105.1"
                     /translation="RPDVAPALTGAVDLPLADPWKLELLADLSTSTLRRHDAGDGTAP
                     ITRLWTLGVSVGLRRRLNPWLEGRAAVGGLKYLPAASIGLFSAGTGGVMPYGSVALDA
                     APAVLAQRRLALELAADVHRFLTPALRNAGFTDARLVYRLSAGVRLDLRRGRAS"
BASE COUNT          230 a          497 c          541 g          190 t
ORIGIN      
        1 gcgcgccgat gtcacccaga ccgagcaccg cggtgccgtt cgagaccacg gccacgaggt
       61 taccccgggt ggtatacagg tagacgtcct cggggcgcct ggcgatctcg aggcacggct
      121 ctgccacccc gggcgtgtag gcgagggaga ggtcgcgctg gttcttgaag ggcttggtcg
      181 gcgagacctg gatcttccca gggcggccct gcgagtgata atcgagggcc tcctggcggc
      241 ggctggacat cgagttccag aagtcctttc cagggaagga gttatgctag gtggacgggg
      301 cgggggtgtc aagccgaagc gccgtattcc caggcgcgcg tgggacttac ggtcggcgcg
      361 gcggcggcgc gagcgtcgcg ggcgtgtgat acagcacctg taccgtgcgg cggtcgatgg
      421 cggccggctc ggccacgagc ggcggcgagc gcatcgcgga gagcgtatcc gccgtctcct
      481 gaaacagccc gaaggaatgc ccgagctcgt gcagtgtcgt gcgccggacg catgccgcta
      541 cctgctcggg cgtgaacacg gtgcccgtga gaatgctgat ctcggtctcg aacggcgccg
      601 cgaacgcctg gccggtcccg tccagcacac cttgggtgac gccgccgcag gcttcgaccg
      661 gcggacccgg atcgggcgta gccgcgggcg gcacactgtc ggcccaacgc acgatcacgt
      721 cggcgcggtt cgagtccgcc accatgacca ccgtgagctc accgtagagc agctgccggg
      781 cccagagatc gaccgcccgg ctgaccagcg cggatagatc cccgcgcgga tcggcgtaga
      841 tgtgtacggg gagccgctcg gccggccaat ggaacacgtc gccgaaggca tcggcgaacg
      901 gatagatcgc cgagcgactc ggcaccaggg gatcggtgca gccggcggcc gccagcacgg
      961 tccccagcac cgcccagcgc ccccctctca cgatgccctc ccgcgccgca ggtccagtcg
     1021 cacgccggcg ctgaggcgat acacgagccg ggcgtcggtg aatccggcgt tgcgcagcgc
     1081 cggggtgaga aaccgatgca cgtcggcggc cagctcgagc gccaggcggc gctgtgcgag
     1141 gacggccggc gcggcatcca gcgcgaccga gccgtacggc atcacgccgc cggtgccggc
     1201 gctgaacagg ccgatagagg cggccggcaa gtacttcaag ccgcccacgg cggcacgccc
     1261 ttcgagccag ggattgagcc ggcggcgcag gcctaccgac acgccgagcg tccacagcct
     1321 ggtgatggga gcggtgccgt cgcccgcgtc gtgccgccgc agggtgctcg tcgagagatc
     1381 ggcgagcagc tcgagcttcc agggatccgc caggggcagg tccacggccc ccgtgagcgc
     1441 gggcgccacg tcgggtcg
//
LOCUS       DBNGZW010000211         2561 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_19364, whole genome shotgun sequence.
ACCESSION   DBNGZW010000211 DBNGZW010000000
VERSION     DBNGZW010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2561)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_19364"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..966
                     /locus_tag="AC16JC_05085"
     CDS             <1..966
                     /locus_tag="AC16JC_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR5164106.1"
                     /translation="EVMYEGAPDHPAKDRFWSICEQHGVTVFYTAPTAIRAFMKWGTD
                     LPAKHNLSPLRLLGTVGEPINPEAWVWYHQHIGGGRCPIVDTWWQTETGGIMITPLPG
                     WTTCKPGSATVAFPGIDAQLLDGEGKPVSVGGGYLAITSPWPGMLRTIWGDDERYRQV
                     YWSRWPGMYFPGDGAKRDEQGYFWILGRVDDVLNVAGHRIGTMEVESALVDHPAVAEA
                     AVVGRSHELKGQALAAFVTLKEGRTATDALREELKSHVAKKIGALARPDDILFSADLP
                     KTRSGKIMRRLLKDIAEGRALGDTTTLADPTVVARLKDQYEAQES"
     gene            complement(1021..1476)
                     /locus_tag="AC16JC_05090"
     CDS             complement(1021..1476)
                     /locus_tag="AC16JC_05090"
                     /inference="COORDINATES: protein motif:HMM:NF019499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HIR5164107.1"
                     /translation="MRHRQAIALLALIGFGIALYLWLYKMGYIGRLQCGTGSCEYVQT
                     SSYGDLWGVPVAFYGVVGYAVIFAIALAGLRPALLSRRWPTQLVAILAAGGWLFTLYL
                     TYVELFVLHAICRWCVSSAVVMTLIAGVAIPAAVKRERGVGSGERSPTV"
     gene            complement(1476..2150)
                     /locus_tag="AC16JC_05095"
     CDS             complement(1476..2150)
                     /locus_tag="AC16JC_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR5164108.1"
                     /translation="MKRFYLLLGLVAVVGGVLIWQASRPSSGGPPASEDAAPVRITAA
                     DSAFRGYVLGSDSAPVEIVEYADYECPVCAQFGAVQFPDIRQQLIESGRVRWRFRDFP
                     LPMHRWSRLSAMVVACASEQGKAWEAMDALFQGHGEWAQRSPDPTGLFRDRMRAIGLD
                     MQAYGACMDAKRYAGRIEASRQEGLARNVGGTPTFFVNGREYNYRRATSDEFLALVDS
                     LTGVRR"
     gene            complement(2147..2449)
                     /locus_tag="AC16JC_05100"
     CDS             complement(2147..2449)
                     /locus_tag="AC16JC_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164109.1"
                     /translation="MHDQTYGPERARRLVALLRSVASRIQELDRDGRMLEAAPELQRL
                     LGNARSELFHYEVRVTYDTPEIAESRRIVEEAERHLGDLGLDEPDDDQPWRKDDDG"
BASE COUNT          455 a          910 c          833 g          363 t
ORIGIN      
        1 gaagtgatgt acgagggcgc cccggaccat cccgccaagg accgtttctg gtcgatctgc
       61 gagcagcacg gcgtcaccgt gttctacacg gcgcccacgg cgatccgggc gttcatgaag
      121 tggggcaccg acctcccggc caaacacaac ctgtcacccc tgcggctgct cggtacggtg
      181 ggtgagccca tcaaccccga ggcctgggtg tggtaccacc agcacatcgg tggcgggcgc
      241 tgcccgatcg tggatacgtg gtggcagacc gagacgggcg ggatcatgat cacgccgctc
      301 cccggctgga ccacctgcaa accgggatcg gcgacggtcg cctttccagg catcgacgcc
      361 cagctgctgg acggcgaggg caagccggtg tcggtgggcg gcggctacct ggcgatcacg
      421 agcccgtggc ccgggatgct gcgcaccatc tggggcgacg acgagcgcta ccgccaggtg
      481 tactggagcc gctggcccgg gatgtatttc cccggggacg gggccaaacg cgacgagcag
      541 ggctacttct ggatcctggg ccgtgtggac gacgtgctga acgtcgccgg acaccgcatc
      601 ggtacgatgg aggtagagag cgccctggtg gatcaccctg cggtggccga agccgccgtg
      661 gtgggccgga gccacgagct gaaggggcag gccctcgccg ccttcgtgac gttgaaggaa
      721 gggcgcacgg cgaccgacgc gctgcgcgaa gagctgaagt cgcacgtggc gaagaagatc
      781 ggggccctgg cgcgaccgga cgacatcctg ttcagcgccg acctgcccaa gacgcgcagc
      841 ggcaagatca tgcggcggct gctcaaggat atcgcggaag gacgcgcgct cggcgacacg
      901 accaccctgg ccgacccgac ggtcgtcgcg cgactcaagg atcagtacga ggcacaggag
      961 agctgatcga aagacgggga gtggggagtg gtcgaagacg gcgcggcgtt gaagtccgcg
     1021 ctacaccgtc ggcgaccgct ccccgctccc cactccccgc tcccgtttca ccgccgccgg
     1081 gatcgccacc cccgcaatca gggtcatcac caccgcgctg ctcacgcacc agcgacagat
     1141 cgcgtgcagc acgaacagct ccacgtaggt gagatacagg gtgaacaacc agccgccggc
     1201 ggcgaggatc gcaacgagct gcgtgggcca gcggcggctc agcagggccg gccgcagtcc
     1261 cgccagcgct atcgcgaaga tcacggcgta ccccacgact ccatagaacg ccaccggcac
     1321 gccccacagg tcgccgtagg agctcgtctg gacgtactcg cagctccccg tgccgcactg
     1381 cagccgcccg atgtagccca tcttgtacag ccagaggtac agcgcgattc cgaacccgat
     1441 caacgcgagc agcgcgatcg cctgccggtg acgcactagc ggcgcacgcc ggtgagactg
     1501 tccaccaggg ccaggaactc gtcggaggtg gcgcgccggt agttgtactc ccgcccgttc
     1561 acgaagaacg tcggcgtgcc gcccacgttg cgcgcgaggc cctcctggcg actcgcctcg
     1621 atgcggccgg catagcgctt ggcgtccatg cacgccccat atgcctgcat gtcgagtccg
     1681 atggcccgca tccgatccct gaacaagccg gtcggatcgg gggaccgctg agcccattct
     1741 ccgtgcccct ggaacagcgc gtccatggcc tcccacgcct tcccctgctc gctcgcgcag
     1801 gcgaccacca tcgccgacag gcggctccag cggtgcatcg gaaggggaaa gtcacggaag
     1861 cgccagcgga cgcgccccga ttcgatcagc tgctgacgga tgtccgggaa ctgcaccgcc
     1921 ccgaactggg cgcacactgg acactcgtaa tccgcgtact ccacgatctc taccggcgcg
     1981 ctgtcgctgc cgaggacgta gccgcggaac gcgctgtccg ccgcggtgat gcgcaccggg
     2041 gcggcatcct ctgaagcagg cggtcccccc gacgacggcc gggaggcctg ccagatgagc
     2101 accccgccca ccaccgccac caggcccaac agcaggtaga accgcttcat ccgtcgtcgt
     2161 ccttccgcca aggttgatca tcgtccggct cgtccagacc caggtcgccg aggtgccgct
     2221 cggcttcctc cacgatgcgc cggctttcgg cgatttcggg cgtatcgtag gtcacacgaa
     2281 cctcgtaatg gaacagctcg ctccgcgcgt tgcccagcag acgctgcagc tcgggcgccg
     2341 cctcgagcat gcggccgtcc cggtccaact cctgtatccg tgacgccacg ctgcgcagca
     2401 gcgccacgag acgacgcgcg cgctcagggc cgtaagtctg gtcgtgcatg ctcacctcgc
     2461 attataccac gcccgtttgc ctggaaagga gggccccatt agattgcgcc cccgacagct
     2521 gcccatcccg aaaggagcgc ctccgaatgg ccaaaaaccg t
//
LOCUS       DBNGZW010000212         5047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_422769, whole genome shotgun sequence.
ACCESSION   DBNGZW010000212 DBNGZW010000000
VERSION     DBNGZW010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5047
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_422769"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..446
                     /locus_tag="AC16JC_05105"
     CDS             <1..446
                     /locus_tag="AC16JC_05105"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5164110.1"
                     /translation="LGVLRPGTLAIHCVQLDAGDIHLLKASRVAVAHCPRSNRAHAHG
                     SAPISALREAGVPVGIGTDSLVSVPDLDVWAEARVSWLGAEESLRILTLDGARALDWE
                     REIGSLEVGKAGDLAVFTHAPPQRLPPPPNALLTVIGGRVVHRVA"
     gene            587..1096
                     /locus_tag="AC16JC_05110"
     CDS             587..1096
                     /locus_tag="AC16JC_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR5164111.1"
                     /translation="MVPVRRALRLVIDGKAEIVEADKGEVMRSARLELPRPAVIRLTK
                     FVHVPRRFRRQVINTFLFARDEYRCQFCGRHQNQLRHRECLTRDHLIPLSRGGSNGWN
                     NVLTACSTCNTRKGNLLPEEVGMHPLRAPVEPHFVHLAWAVRRLTPTQSRYIRLFYGA
                     AVLRTLEEL"
     gene            complement(1141..1761)
                     /locus_tag="AC16JC_05115"
     CDS             complement(1141..1761)
                     /locus_tag="AC16JC_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164112.1"
                     /translation="MRRVVVVPGLLAVAMLRFAARDPGLHLTRDGPVARVQASGLLDD
                     GKFVELMQSGFPLRLHFRLELWKNRSGWFDRYITGVDWDAVARDDPLAEEFVLIRTGG
                     GVTRYNTTEDLAAALSVPYRVTLAARGSGRFYLVARLDVTTLNDTDLEELTRWLRGDV
                     TPAVSGRENLGQALAHGAQRALVRIAGLPTLTLEARSDVFPGEDSR"
     gene            complement(1764..3515)
                     /locus_tag="AC16JC_05120"
     CDS             complement(1764..3515)
                     /locus_tag="AC16JC_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR5164113.1"
                     /translation="MSPRLLVVTALGAALSFARPLVAQDTAIVIHPESAGLHLTPPEL
                     PHAVVTQAVQFYNAPTTTHLVGRVRLPAGNTWAGNVAIRNGPAFIAGTVDGTMLILNG
                     TVTLDSTARVTGDLIVIGGTLLGVREAVAGSVHLYAEPLPYRLVGDTLIHAPDLARRF
                     SLAARYVFGRGSSRSSLILATGGTYNRVEGLPIVAGPTVDLTLGRGVALLGSAIGVFR
                     TARSISGECCDLGYNTRLELRLGDRRPVSLALRAFDLVTPVEDWGLHSNEVGWEAFLV
                     RRDYRDYYRDNGVGVRLGWQAEAPLALGVELRRERQASLAARDPWTLSRTGRPWRPNP
                     PVDNGHYTTVSPGVTFDTRNDRSQPTSGWYLRATFDVISSHDVRPAPGMPSAVRDSTT
                     LLGAYRSTRAFLDFRRYNRLSAQGRLNLRLVLAGWTGGDPLPLQKRLSIGGPEPLPGY
                     DFRQGACSALVTDAAYAGTNVAICDRVVVVQAEYRGHVALRTRYNPETGEGEGVGYVT
                     RFLSGPDFVVFGDAGQAWLVGTGPSRVPSGRLPGLGSWQADFGLGVDWGGFGVYLAKA
                     VTAGQPVRLTARLEHRF"
     gene            complement(3512..4894)
                     /locus_tag="AC16JC_05125"
     CDS             complement(3512..4894)
                     /locus_tag="AC16JC_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5164114.1"
                     /translation="MSPRVLLVDDEHNIRRMVGALLQEEGYEIADAPNGAAALAAVRE
                     TPPDAILLDLMMPGGPDGMATLEELKRTLPDVPVVMMSGKASLADAVRATKLGAFQFL
                     EKPLTPEGLLVAVRSALELSRARAENRRLHEALGHADPLVGGSAAMDELRALIARIAP
                     TDARVLITGESGTGKELVASAIHRQSGRAAKPFVTVNSAAIPRDLVESELFGHEKGAF
                     TGAGERRLGRFELADGGTLFLDEVGDLGQEAQAKLLRVLETGVIERVGGERPVTVDVR
                     VIAATNKDLARATREGKFRDDLLFRLNVLPVHIPPLRDRTEDVAPLVRHFLARQAARL
                     GRSFTVDAGALQLLAAYRWPGNVRELGNLMERLAILAATDAITAEEVLRVLPPDAVSA
                     RVVGTAPPGADVSLAEALDRYERQLIAAALSAATGNVADAARRLSTDRANLYRRMRRL
                     GLEPPRHLAE"
     gene            complement(4891..4983)
                     /locus_tag="AC16JC_05130"
     CDS             complement(4891..4983)
                     /locus_tag="AC16JC_05130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164115.1"
                     /translation="MRQTVDAHHGTIAVAETEGGGATFTLVFPA"
BASE COUNT          725 a         1820 c         1789 g          713 t
ORIGIN      
        1 agctgggggt gctgcgcccc ggtaccctgg cgatccactg cgtccagctc gacgcggggg
       61 acatccacct gctcaaggcg tcgcgcgtgg ccgtcgcgca ctgtccccgc tccaaccgcg
      121 cccacgcgca cggctcggcg cccatttcgg cgttgcggga agcgggagta ccggtcggga
      181 tcggcaccga ctcgctggtg agcgtgcccg atctggacgt ctgggccgag gcacgggtat
      241 cgtggctcgg ggccgaggag tctctgcgca tcctgacact ggatggcgct cgggcgctgg
      301 attgggagcg ggagatcggc tccctggaag tcggcaaagc gggggacctg gcggtgttta
      361 ctcacgcccc gccgcaacgc ctgccgccgc cgcccaacgc cctcctgacc gtgatcggcg
      421 gccgcgtggt gcaccgcgtc gcttaacccc cggtcttgcc gaacgttaga agtccgagta
      481 ggatgttggg accgcccgtt ccaggcgagc gaggcggccg tttttgtgtc agacgaggta
      541 acccgtgcgt tgtctagcgc ttaacgcgtc gttcgagccc ctgaccatgg tgcccgtacg
      601 ccgcgccctg cggctcgtga tcgacggcaa ggccgagatc gtcgaggccg acaagggcga
      661 ggtgatgcgc agcgcacgcc tcgagctgcc ccggccggcc gtgatccgtc tcacgaagtt
      721 cgtgcacgtg ccgcggcgct tccgccgtca ggtgatcaac accttcctgt tcgcccgcga
      781 cgagtaccgt tgccagttct gcggtcgtca ccagaaccag ctgcggcacc gcgagtgcct
      841 cacccgcgac catctgatcc ccctgtcgcg cggcgggtcg aacggctgga acaacgtgct
      901 gacggcgtgc tcgacctgca acacgcgcaa gggcaacctc ctccccgagg aggtgggcat
      961 gcacccgctg cgcgcgccgg tggagccgca tttcgtgcat ctcgcctggg cggtgcgccg
     1021 cctcacaccc acgcagtccc gctacatccg gctgttctac ggcgcggcgg tgctgcggac
     1081 gctcgaggag ctgtgaccgg gcgggcgatc gacgcccgct acggcggctg agcccccccg
     1141 ctagcggctg tcctccccgg ggaatacgtc cgagcgcgct tccagggtca gcgtgggcaa
     1201 tccggcgatc cgcaccagcg cccgctgcgc cccatgcgcc agcgcctgcc ccaggttctc
     1261 ccggcccgac accgccggcg tgacatctcc ccgcagccag cgcgtcagct cttcgagatc
     1321 cgtgtcgttc aacgtcgtca cgtccagccg cgcgaccaga tagaagcggc cgctcccgcg
     1381 ggccgcgagc gtcacccggt acggcacgga caaggccgcg gcgaggtctt cggtcgtgtt
     1441 gtagcgggtc acgccgccgc cggtccggat gaggacgaat tcctccgcca acggatcgtc
     1501 ccgtgcgacg gcgtcccagt ccacgccggt gatgtagcgg tcgaaccatc cggagcggtt
     1561 cttccacagc tccagccgga agtgcagccg gagcgggaag ccggattgca tcagctccac
     1621 gaatttcccg tcatcgagca gccccgacgc ctgcacgcgg gcgaccgggc cgtcccgcgt
     1681 caagtggaga ccggggtccc gggcggcgaa ccgcagcatc gccacggcga ggaggccggg
     1741 cacgaccacg acgcgacgca cgattagaac cggtgctcca gccgtgcggt caggcgtacc
     1801 ggctgaccgg ccgtcaccgc cttggccagg tacacgccga acccgcccca gtccacgccg
     1861 agcccgaagt cggcctgcca gctgcccagt ccggggagcc ggccgctcgg cacgcggctc
     1921 ggccccgtgc ccaccagcca ggcctgtccg gcgtccccga agaccacgaa gtccggtccg
     1981 ctcaggaagc gggtgacgta gcccactccc tccccctcgc cggtctccgg gttgtaacgc
     2041 gtacggagtg cgacgtgccc gcgatattcc gcttgcacca cgacgacccg gtcgcagatg
     2101 gcgacgttgg tgccggcgta ggcggcgtcg gtcacgagcg cactgcacgc cccctggcgg
     2161 aagtcgtatc ccggcagggg ctccggtcct ccgatgctga ggcgcttctg cagcggcagc
     2221 ggatcgcccc cggtccatcc ggccagcacg aggcggaggt tcagccgccc ctgagcgctc
     2281 agccggttgt agcggcggaa gtcgaggaac gcccgcgtgg agcgatacgc cccgagcagc
     2341 gtggtcgaat cgcggacggc gctcggcatc ccgggcgccg gccggacgtc gtgactcgag
     2401 atgacgtcga acgtggcgcg gagataccac cccgacgtgg gctgggagcg gtcgttgcgc
     2461 gtgtcgaagg tcacgccggg ggacaccgtg gtgtagtgcc cgttgtcgac cggcggattg
     2521 ggccgccacg gcctcccggt gcgcgacagg gtccagggat cgcgggcggc gaggctcgcc
     2581 tggcgctcgc gccgcagctc cacgcccagc gcaaggggcg cctccgcctg ccagccgagc
     2641 cgcacgccga ccccgttgtc gcggtagtaa tcgcggtagt cccgccgcac gaggaacgcc
     2701 tcccacccca cctcgttgga gtgcagcccc cagtcctcga ccggagtgac gaggtcgaac
     2761 gcgcgcagcg ccagggaaac cggacgtcgg tcgccgaggc ggagctcgag tcgggtgttg
     2821 tagcccaggt cgcagcactc gccgctgatc gagcgtgccg tgcgaaacac cccgatcgcg
     2881 gacccgagca gcgccacgcc gcgacccagc gtgagatcca ccgtcggccc ggccacgatc
     2941 ggcaggccct cgactcggtt gtaggtcccg ccggtggcga ggatcaggct cgagcgagac
     3001 gagccgcgcc cgaagacgta acgcgccgcg agactgaagc ggcgggcgag gtcgggggca
     3061 tggatcagcg tgtcacccac cagccggtag ggcaacggct cggcgtagag gtgcacggaa
     3121 ccggccaccg cctcccggac gccgagcagc gtgccgccga tcacgatgag atcgccggtg
     3181 acgcgggcgg tggagtcgag ggtgacggtg ccgttgagga tcagcatcgt gccgtccact
     3241 gtgcccgcga tgaacgccgg gccgttgcgg atggcgacgt tgcccgccca ggtgttgccg
     3301 gccggcagac ggacgcggcc cacgagatgc gtcgtggtcg gggcgttgta gaactgcacc
     3361 gcctgcgtca cgacggcgtg cggcagctcg gggggagtga gatgcagtcc cgcgctctcg
     3421 gggtggatga cgatcgcggt atcctgcgcg acgagcggtc gggcgaagct gagcgcggcg
     3481 ccgagcgccg tgacgacgag gaggcgaggg ctcattccgc gaggtggcgc ggcggctcga
     3541 ggccgagccg gcgcatccgt cggtagaggt tggcgcggtc ggtggacagg cggcgcgcgg
     3601 cgtcggccac gttgccggtg gctgcggaga gcgcggccgc gatcagctgc cgctcgtagc
     3661 gatcgagcgc ctcggcgagc gaaacgtcgg cgcccggcgg cgcagtcccc acgaccctgg
     3721 cggagacggc gtcgggcggg aggacccgca gcacctcctc ggcggtgatc gcgtcggtcg
     3781 ccgcgaggat cgcgaggcgc tccatcaggt tcccgagctc ccgtacgttg cccggccagc
     3841 ggtacgccgc caggagctgc agcgcaccgg catccacggt gaaactgcgg ccgaggcgcg
     3901 ccgcctggcg agcgaggaag tgccggacca ggggcgcgac gtcctcggtg cggtcgcgca
     3961 gcggggggat gtgcaccggc agtacgttga gccggaacag caggtcgtcg cggaacttcc
     4021 cttcgcgtgt cgcgcgcgcc aggtccttgt tggtggcggc gatgacccgc acgtcgaccg
     4081 tgaccggccg ctcgccgccg acccgctcga tcactccggt ctcgagcacg cgcagcagtt
     4141 tcgcctgcgc ctcctgcccc aggtcgccca cctcgtcgag gaacagcgtc ccgccgtccg
     4201 ccaactcgaa ccgtcccaga cgccgctcac ccgcaccggt gaaagcgccc ttctcgtggc
     4261 cgaacagctc cgattcgacc aggtcgcggg gaatcgccgc gctgttcacg gtcacgaacg
     4321 gcttggcggc ccggccgctc tgacggtgaa tcgccgacgc caccagctcc ttgccggtgc
     4381 ccgattcccc ggtgatcagc acgcgggcgt cggtcggtgc gatgcgcgcg atcaacgcgc
     4441 gcagctcgtc catcgccgcg ctccccccta cgagcgggtc ggcgtgcccc agcgcttcgt
     4501 gcagccggcg gttctcggcc cgggcccgcg acagctccag cgcgctccgc acggcgacga
     4561 gcagcccctc gggggtgagc ggcttctcca ggaactggaa ggcgccgagc ttggtggcgc
     4621 gcacggcgtc ggcgagcgac gccttgccgc tcatcatcac gacggggacg tcggggagcg
     4681 tccgcttcag ctcttcgagc gtcgccatgc cgtccggtcc ccccggcatc atgagatcca
     4741 gcagaatcgc gtcgggcggg gtctcgcgca ctgcggccag cgccgcggca ccattcggcg
     4801 cgtcggcgat ctcgtacccc tcctcctgga gcagcgcccc gaccatgcgg cggatgttgt
     4861 gctcgtcgtc gaccagcagc acgcgcgggc tcatgcggga aacaccagcg tgaaggtggc
     4921 gccgccgccc tcggtttccg ccacggcgat cgttccgtga tgggcgtcca ccgtctggcg
     4981 caccagcgcc agaccgagtc ccgtcccgtc cggcttggtc gtcagatagg gctcgaatac
     5041 gcggccg
//
LOCUS       DBNGZW010000213         2978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1245221, whole genome shotgun sequence.
ACCESSION   DBNGZW010000213 DBNGZW010000000
VERSION     DBNGZW010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1245221"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..845
                     /locus_tag="AC16JC_05135"
     CDS             <1..845
                     /locus_tag="AC16JC_05135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR5164116.1"
                     /translation="GRLFLSHMGAGQLVVFDTRGAKVVRTLDGFPTVTGVLAVAVEHR
                     VYASVAGAHAVVVVDDSTLETVARVPGPEFPDGIAYAPPARRLFVSDESGRREFVLDA
                     TTNAVVARVGLGGEAGNSQYDPVSRCVIVAVQTEQQLAVIDPTTARVVRRITLAQAVR
                     RPHGIAIDAPHRLAFIAGETSATLGVLDLGTLRLRQVLPVGTDPDVLAFDPALGRLYV
                     AAESGVLAVFEERGGSLVQLGWYRAPHAHSVAVDPVTHRVFLPIANLDGRPVLRILLP
                     ADRR"
     gene            complement(932..1723)
                     /locus_tag="AC16JC_05140"
     CDS             complement(932..1723)
                     /locus_tag="AC16JC_05140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164117.1"
                     /translation="MNWSTLVAPKVSAGVAFGVLALGVGACRQERLVQGECRSLHGAD
                     ICAWDRMAGKAVVAFGATVPMRAIDSAPAEVPMAWPPVAAATIPLSPAAQAASGFDNI
                     TIFWEPHGHPPGPYLMPHFDFHFNTISAAELGAIDCADSTRPTQLPGSYELPDVDIPG
                     LGRLVGLCVPTMGMHSMPVAELHATTPFEKTMVVGYYRGQPIFIEPMVTRSALLARRT
                     FQLAVPQVPNGPANVRYPRQFMAEYDSTAQQYRFVFSGLGGGTTQ"
     gene            1852..>2978
                     /locus_tag="AC16JC_05145"
     CDS             1852..>2978
                     /locus_tag="AC16JC_05145"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HIR5164118.1"
                     /translation="MRAVALVLSTSLITLGLARVPRAVLDNGQARTPPMGWNSWNHFG
                     CDVSSQLIRETADAMVASGLREAGYRYVVIDDCWQVARDARGRLVADSGRFPGGIKPL
                     ADYVHSRGLKFGIYTDAGRRTCQGRPGTYGSEEIDARTFADWGVDYVKEDWCYAEGLD
                     APTQYAKFRDALAKAGRPMVLSICEWGSFNPWDWGPTTGNLWRTTTDIEDTWASMLSN
                     LDISGQHGAAARPGAWNDPDMLEVGNGGMTEDEYRAHLSLWAIMAAPLIAGNDIRSMS
                     AATRDMLTNSEVIAVDQDSLGVQGTLVSERTPELQVWMKPLKDGSRAVVLLNRSALQT
                     VISASWWRLRISGPARVRDLWSHADLGTFTDRFSATVPAHGV"
BASE COUNT          444 a         1017 c         1044 g          473 t
ORIGIN      
        1 tgggccgact cttcctctcg cacatggggg cgggacaact cgtggtgttc gacacgcgtg
       61 gcgcgaaagt cgttcggacc ctggacggct ttccgacagt caccggcgtc ctggccgtag
      121 cggtcgagca tcgcgtctat gcgtccgtcg ccggagcgca cgccgtcgtg gtggtggacg
      181 actcgacgtt ggagaccgtg gcgcgcgtgc cggggccgga attcccggac gggatcgcct
      241 atgcgccgcc agcgcggcgc ctgttcgttt cggacgagtc ggggcggcga gagttcgtgc
      301 tggacgcgac cacgaacgcc gtcgtggcgc gggtaggact gggtggggag gcaggcaaca
      361 gccagtacga ccctgtttca cgctgcgtga tcgtggcggt ccagaccgag cagcaactcg
      421 cggtgatcga tcccaccacg gcccgggtag tgcgccggat cacgctcgcc caggctgtcc
      481 ggcgtccgca cggcatcgcc attgacgcgc cgcaccgact ggcgttcatc gccggggaaa
      541 ccagcgcgac cctcggggtc ctggatctcg gcacgctgcg tctccgccag gtgctgccgg
      601 tcggtaccga ccccgacgtc ctggcgttcg atcccgcgct cggccggctc tacgtggccg
      661 ccgagtccgg ggtcctcgcc gttttcgagg agcggggtgg atcgctcgtc cagttgggct
      721 ggtatcgcgc gccgcacgcc cattcggtcg cggtcgatcc cgtgacgcac cgagtcttcc
      781 tgccgattgc caacctggat ggtcgtccgg ttctccggat cctgttgccc gccgataggc
      841 gctgaccgcg gcgccgtgca ttcgcctccc gcaccgaccg ggcgccaccg ggaaccacca
      901 gcgggcgcac ggacggtcgg gtcgccctgg cttactgcgt cgtgccccct cccaagccgg
      961 agaatacgaa gcggtactgc tgcgcggtgc tgtcgtactc cgccatgaat tgtctgggat
     1021 accgcacatt cgctgggccg ttcggaacct gcgggaccgc gagctggaag gtgcggcgcg
     1081 ccagcagggc ggagcgggtg accatcggct cgatgaagat cggctgcccg cggtagtacc
     1141 ctacgaccat cgtcttctcg aagggcgtcg tggcatgcag ctcggcgacg ggcatcgaat
     1201 gcatccccat ggtcggcacg cacaggccca cgagcctgcc gagacccggg atatccacat
     1261 ccgggagctc gtagctcccc gggagctggg tcggcctggt cgagtcggcg cagtcgatcg
     1321 cgcccagctc cgccgccgag atcgtattga aatggaaatc gaaatgcggc atgaggtagg
     1381 gacccggcgg atggccgtgg ggctcccaga agatggtgat gttgtcgaaa ccggacgccg
     1441 cctgcgctgc cggggagagg gggatggtcg cagccgccac gggcggccac gccatcggca
     1501 cctcggccgg ggcgctgtcg atcgcccgca tcggcacggt ggccccgaac gcgaccaccg
     1561 cctttccggc catgcgatcc caggcgcaga tgtccgcgcc gtggagcgac cggcattctc
     1621 cctgcacgag ccgttcctgc cggcacgcgc cgacacccag ggcgaggact ccgaacgcga
     1681 ctccggcgga taccttcggt gcgaccagcg tgctccagtt catgatcatt ccagtgtggg
     1741 gtggtagggg cggcgaaggt acgcgccaat cggtcccggt caagcgtcgt gctcgcggcg
     1801 cttcccctgc acacgttagt attgctgctc aacaccctcc gagagccgtc catgcgtgcc
     1861 gtcgcgctcg ttctttcgac ctccctgatc acgctcggtc tcgcgcgggt cccgcgggcc
     1921 gtactcgaca acggccaggc ccgcaccccg cccatgggct ggaacagctg gaaccacttc
     1981 ggctgcgacg tgagcagcca gctcatccgg gagacggccg acgccatggt ggcgagcggg
     2041 ctgcgcgagg cgggctaccg ctacgtcgtc atcgacgact gctggcaagt ggcgcgcgat
     2101 gcgcgcggcc ggctggtcgc cgattccggg cgcttccccg gcggcatcaa gcccctcgcc
     2161 gactatgtcc actcgcgtgg cctcaagttc gggatctaca ccgacgcggg tcgcaggacg
     2221 tgccagggcc ggcccgggac ttacggctcc gaggagatcg acgcccgcac gttcgcggac
     2281 tggggcgtgg actacgtgaa ggaggactgg tgctacgccg aggggctcga cgcccccacg
     2341 cagtacgcga agttccggga cgcgctcgcc aaggcggggc gccccatggt cctgagcatc
     2401 tgcgaatggg gatcgttcaa tccctgggat tgggggccca ccaccggcaa cctgtggcgc
     2461 accacgaccg acatcgagga cacgtgggcc agcatgctct cgaacctaga catctccggc
     2521 cagcacggcg ctgccgctcg tccgggggcg tggaacgacc cggacatgct cgaggtcggc
     2581 aacggcggga tgaccgagga tgaataccgc gcccacttga gcctctgggc gatcatggcc
     2641 gccccgctca tcgcggggaa cgacatccgg tcgatgtcgg cggcgacgcg ggacatgctc
     2701 acgaacagcg aggtcatcgc ggtggaccag gattcgctcg gcgtccaggg cacgctggtg
     2761 agcgagcgaa ctccggagct gcaggtgtgg atgaagccgc tcaaggacgg ctcccgggcc
     2821 gtcgtgctgc tcaaccgctc cgcgttgcag acggtgatct cggcgtcgtg gtggcggctg
     2881 cggatcagcg gcccggcgcg cgtgcgggat ctgtggtccc acgccgacct gggcaccttc
     2941 accgaccggt tcagcgcgac ggtgccggcc cacggcgt
//
LOCUS       DBNGZW010000214        11691 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_291806, whole genome shotgun sequence.
ACCESSION   DBNGZW010000214 DBNGZW010000000
VERSION     DBNGZW010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 11691)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11691)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11691
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_291806"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="AC16JC_05150"
     CDS             complement(<1..403)
                     /locus_tag="AC16JC_05150"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019512703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5164119.1"
                     /translation="MAVRDASFSVRRGEFFAVLGPSGCGKTTTLRMIAGFEEPTQGRI
                     LLEGRDVSRVPPYRRRVNTVFQQYALFPHMSVADNVAFGPRSRNLGDADVARRVREVL
                     AVVQLEDLAHRKPAQLSGGQQQRVALARALVN"
     gene            complement(512..2053)
                     /locus_tag="AC16JC_05155"
     CDS             complement(512..2053)
                     /locus_tag="AC16JC_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR5164120.1"
                     /translation="MIRFTHLTGSLQGTATSSEKRWLRVGTGHDCELRYDAAAEPKVA
                     PYHAAVVLKDGAYHVMDLDAPGGIRVNGKKVAKVELRSGDTIRFGATGGPEAQVEIVI
                     DASYDPAQDAAEITKVLQAGGRASATGQIFALTAQKIAEERAKAGGVRSRKTMDYVAT
                     AVQEVSEVVKKQTKKRWVKVLGVVSAAAVVVIGVMGTVIYVQHRQIARLLDAKGRFDV
                     QIQDIQRQMQAESDSTRLALLEQRYLDATSNAERTLADLARADRAKAVEVANAGDELD
                     REIRAILRQFDAETYAVPPIFKERLRYHIDLLLKSNVATREVYQRKVQYWPVISKNFR
                     ALELPDVMGYVAWTESRFDPNAHSRAGAVGMWQMMAPSARDLGLRVEGKVDERTDVDK
                     QTKAAARHLANLLAEFGEDAFMLAMASYNRGENGVRGVLHKIAATPGGFKRQNRDFWH
                     LYRMKLLPPETREYVPKILAAAVICSNPQRYGLEPATALAGTTPGKASPPPASRSSAP
                     SAARR"
     gene            complement(2131..3717)
                     /locus_tag="AC16JC_05160"
     CDS             complement(2131..3717)
                     /locus_tag="AC16JC_05160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164121.1"
                     /translation="MRRLAFLCGVLILPARVSGQHVPAKAMRWAHVTYLTTASAYIDA
                     GRAEGLRDNSRVEVVRGDSTIAVLAVTFLATHQAACDVSGSAASLAVGDSVRYTPAAA
                     EPDSAVAVTRRAPDGRASSRPRAGGVLRGRAGLYYLLIQAQDGSGARFTQPSGDLRLF
                     GGRLGGTGLGIAVDARGRRTVQTRADGFGTDAQAQARLYQASLSWQAPASPLRLTVGR
                     QYAPGIAPVGLVDGVSAQLDRPGWSGGLFAGSEPDPIDLALSGTVTQLGGYLQRHSRP
                     GSGARWGLSAGLSGSYLHSGTNREFLYLQGDYATRRLSLYAAQEVDYYRPWRRVAREH
                     ALSPTSTFASLVYRPSDVVRLTAGLDNRRNVRLYRDVINPETVFDDTYRRGAWAGLSA
                     RAGRFQVGVDARTSVGSTIGSAGSTTLSIGADRLTGWGLSLRSRSTRYSSAAAGRRGW
                     LQAFTVGMEPGGRGSVQVAGGWRSERSAVPGTTATVRWVSSDLDYTLARSWLLVVSAY
                     REQGGPAAQDLLYGGLSLRF"
     gene            complement(3720..4742)
                     /locus_tag="AC16JC_05165"
     CDS             complement(3720..4742)
                     /locus_tag="AC16JC_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164122.1"
                     /translation="MVTTRVLGLAGGLLLVVAGPARAQNRLVANPHGELSVSCNVCHS
                     PEGWTPAHVSAAFDHGKTAGLALVGAHAAVACRACHTTLDFRGTSRSCVSCHADPHRG
                     EVGNDCARCHTARNFLDRSGMARAHQLTRFPLQGTHLTVDCEACHAAPQGRLRFVTLA
                     TECSTCHLKDYRATRDPDHTAVGYTTDCQQCHAAITWLRTNFDHGSTGFALTGAHLRI
                     NCSRCHGVGQFQALPSACVSCHQTDYDATTDPNHVAAGFPTDCSGCHTTARWTGARFD
                     HDRTFFPIYSGAHQGRWTSCSECHINANDYRQFDCVHCHQNAHQGRGYTSQQCYSCHR
                     NGRGEG"
     gene            complement(4921..6993)
                     /locus_tag="AC16JC_05170"
     CDS             complement(4921..6993)
                     /locus_tag="AC16JC_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164123.1"
                     /translation="MRTGLEWGSLVGTLLLAAPAWSQQVGSGYKFGRVRSVAEGAVGL
                     DVGSADGVRPWTRLEIIRSGRAVAVLKVSSLSAHEATATITSRPVPLRVGDYVRFLFA
                     NAPVARDTAARLRARGAVAAPARSPVPIPTTASGAPPAPATAPAAPPPAAATAAAPPP
                     AAPAAGAPAPAPAPVGPAVPTAGAPLARITFVTTSSAYLSAGKADGLIEGARVDVVRA
                     GRTVGELKVSFVSTHQASCQIVAAADSVMVGDSARYLPAASTAAPPPAVAEPTQSAAR
                     PAPQRSARRSSTGALRGRIGMYYLTVAQRDSLGGHFAQPSGDLRLSGGGLGGSPLGLA
                     LDVRTRRSVRVLPGTRSSTTDQTRVYQAQVYWQAPGSPVRFTTGRQYAPGITSVGLLD
                     GAAVEINQSGWDYGLFGGLQPELIDLGFSGDISQLGGYVRRHNGAGSLTHWSLTAGAS
                     GSYVTWHTNREFLYLQASYLTRRFSFYGVQEVDYYRPWRRVQGEQAVSPTSSFANLQL
                     QVTGGLGLTAGFDNRRSVRLYHDVIDSTAAAVFDETFRRGVWTGLTVRTPRHFRASFD
                     VRTNHDATTGTANTFTLALAVERLTSLGIGLRTRSTRYTTGPRDGWLSSVSFGLEPWG
                     RGSVALTSGWRTEHDRTAAPALDIRWLSADMDVSLARSLFVVLSAYRETGGLEAHDLL
                     YAGMSYRF"
     gene            complement(6993..8690)
                     /locus_tag="AC16JC_05175"
     CDS             complement(6993..8690)
                     /locus_tag="AC16JC_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164124.1"
                     /translation="MTLRATILWGVLPFGTVRAASAQRPDTRNPHGRLEQPCATCHSA
                     AGWVPAKISASFDHGQSGFPHEGAHASVACKSCHTTLTFKGASRDCASCHKDPHQGEV
                     GSSCARCHTMRSFVDRTGMTRAHQLSRFPLTGAHVTADCESCHTRSTQGHLTFTSRST
                     ACNDCHLRDYQTAKSPDHAGGGFSQSCEQCHATVAWQSARFDHSRSGFPLTGAHLAVN
                     CAQCHGTGRYGALPTTCVSCHQTAYNNAANPNHPQAQFPTDCTACHTTVTWTGATFNH
                     NSTAFPLTGAHVQLTCDQCHGDGVYKGKPTSCVSCHQTAYNNATNPNHPQAQFPTDCT
                     ACHTTTTWTGATFNHSATAFPLTGAHLQATCDQCHGDGVYKGKSTACVSCHQTDFNNA
                     TNPNHVQSQFPTVCTACHSTTTWTGATFNHNATAFPLTGAHLQATCDQCHGDGVYKGK
                     STACVSCHQTDYNSTTDPNHAQAQFSTDCVACHATTVWTTATYTSHDAQYFPIYSGAH
                     AGRWTSCSTCHINSSDYRQFDCLSCHGKTQTDSHHSGVSGYTYDSQACYRCHPRGTH"
     gene            complement(8687..10645)
                     /locus_tag="AC16JC_05180"
     CDS             complement(8687..10645)
                     /locus_tag="AC16JC_05180"
                     /inference="COORDINATES: protein motif:HMM:NF025875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5164125.1"
                     /translation="MSALLLGAPLHAQISPGPLAKPHVQLEGALRCVNCHGGGSKEQM
                     TVRCLECHKEIASLRQQNRGYHARVQQQPCSSCHPDHAGRAFALIAWPERDVARFDHA
                     LAGWPLEGRHATITCADCHKAAFRTGPAAALSPQPSAAPGWVGLETTCTSCHRDVHRR
                     SLGQDCVSCHDTERWSPAPKFDHARSDYPLTGKHRDVRCASCHKPAAGDTVAVFKPVA
                     HRDCAACHTDPHRGRFAGPCSDCHVTTGFKVIDRGNFNHDRTRYPLRGEHVSVTCAKC
                     HDTPGLKGKNPPFATCTSCHADPHGGTATLAGAVVDCAACHTVAGFAVAGFTLEQHAK
                     TKYPLEGRHRQVACVSCHVKNPAGVPAGRLGTAGIQMHPAFARCLDCHRDDHGGQLGA
                     RADGGDCTACHSVAGWKPSTFTVAAHAKARFRLEGAHAEAGCDACHSTQRKGLAPFPR
                     TPTVGKAGVLFRLGELECVACHVDPHQGRFAERGAHPIRGGCLGCHDTRRYRPSTIDV
                     AAHGTYAFKLEGAHRAVPCVGCHADLKYLPAASTLIQARWTGSPLLFTTRTQGCVGCH
                     LSPHGDQFAGRQGGDKCESCHGADAFAPASRFDHDRDTKFPLRGGHANVACARCHATI
                     AGPGNTRNIRYRPVSTRCESCHGDGVRQ"
     gene            complement(10678..11559)
                     /locus_tag="AC16JC_05185"
     CDS             complement(10678..11559)
                     /locus_tag="AC16JC_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164126.1"
                     /translation="MSRSPIRASRFLLLTFVGLLVAICVVGLPYYTQPVAERVRSPFH
                     PWLKPAGYVGQSAGLLAIAIFIFLWLYPFRKKARWLAWTGSVGRWLDVHVLAALSLPL
                     LVAIHAAWRFEGLIGLGFWSMMVVWASGVVGRYLYTRIPRGKAGIELTLEEIGARRRQ
                     LLEKIATSTGLDLQRIEAALDTGRGPSGRSGVVRTLFQLLADDLARRRAARRFRRLYA
                     QSGLRRHNQDRQMFRWVLRLAKREMALTQQGRMLDATHALFRWWHVLHRPVAIAALLA
                     VLVHVTVVVALGATWLW"
BASE COUNT         1749 a         4056 c         4108 g         1778 t
ORIGIN      
        1 ggttcaccag ggcgcgggcc agcgccacgc gctgctgctg gccacccgac agctgggcgg
       61 gcttgcggtg cgccaggtcc tccagctgca ccacggcgag cacctcgcgg acccgccgtg
      121 ccacgtcggc gtcccccagg ttccggctgc gcggcccgaa agcgacgttg tccgccaccg
      181 acatatgggg aaacagagcg tactgctgga acacggtatt cacgcggcgg cggtacggtg
      241 ggacacgaga cacgtctcgg ccctcgagca ggatccgccc ctgcgtcggc tcctcgaatc
      301 cggcgatcat gcgcaacgtg gtcgtcttgc cgcatccgga aggcccgagc accgcaaaga
      361 actcaccgcg acggacgctg aagctcgcgt cgcgcacggc caggctgtca tcgaaccgct
      421 tggaaacgtg ctcgagcgcg acgacgtcct cggcggtggg cgccgtgacg ctcgccgcgc
      481 tgccgggctg gagctgagcc accgatgcga ctcagcggcg ggcggccgag ggagcgctcg
      541 agcgggaggc gggaggaggc gaggctttcc cgggcgtggt cccggcgagg gccgtggccg
      601 gctcgagccc gtagcgctgc gggttgctgc agatcactgc ggccgcgagg atcttgggaa
      661 cgtattccct cgtctccggg ggcaggagct tcatgcggta gagatgccag aagtcgcggt
      721 tctgccgttt gaagccgccc ggagtcgccg cgatcttgtg cagcacaccg cgcaccccgt
      781 tttccccccg gttgtagctc gccatcgcca gcatgaacgc gtcctccccg aactccgcga
      841 ggaggttcgc caggtgccgg gcggcggcct tcgtctgctt gtcgacgtcg gtgcgctcgt
      901 ccactttccc ttccacccgc agcccgagat cccgggcact cggcgccatc atctgccaca
      961 tcccgaccgc cccggcgcgg ctgtgcgcgt tggggtcgaa ccgcgactcc gtccaggcca
     1021 cataccccat cacgtcgggg agctcgagcg cccggaagtt cttcgagatc acgggccagt
     1081 actgtacctt gcgctggtac acctcgcgcg tggcgacatt cgatttgagc agcagatcga
     1141 tgtgatagcg cagccgctcc ttgaagatcg gcggcacggc ataggtttcg gcgtcgaatt
     1201 gccgcaggat ggcccggatc tcgcgatcca gctcgtcgcc cgcgttcgcc acctcgaccg
     1261 ctttcgcccg gtcggcgcgg gccagatcgg ccagtgtccg ctcggcgttg gaggtcgcgt
     1321 ccaggtaacg ctgctccagc aacgccagcc gggtcgagtc gctctccgcc tgcatctggc
     1381 gctggatgtc ctggatctgg acgtcgaagc gccccttggc atcgagtaac cgagcgatct
     1441 ggcgatgctg cacgtagatg accgtgccca tcaccccgat caccactacg gcggccgccg
     1501 aaacgacgcc gaggaccttc acccagcgct tcttggtttg cttcttgacg acttcggaga
     1561 cttcctgcac cgcggtggcg acgtagtcca tcgtcttgcg agaccgcacg ccgcccgctt
     1621 tcgcccgctc ttccgcgatc ttttgcgccg tcagcgcgaa gatctgtccc gtggccgacg
     1681 cccgaccccc ggcctgcagc accttggtga tttccgccgc gtcctgggcc gggtcgtagc
     1741 tcgcatcgat cacgatctcc acctgcgcct cgggaccgcc ggtcgcgccg aagcggatcg
     1801 tgtctcccga acgcagctcg accttggcga ctttcttgcc gttcacccgg atgccgcccg
     1861 gggcgtcgag atccatgacg tggtaggcgc cgtccttgag caccacggcc gcgtggtacg
     1921 gcgccacctt cggctcggcg gcggcatcgt agcgaagctc gcagtcgtga cccgtgccca
     1981 cgcgcagcca gcgcttctcg ctgctcgtgg ccgtgccctg gagcgagccc gtgaggtgtg
     2041 tgaagcggat catcggatgg cggaagatta ccggccgcgc cccggatgcg ctagttggac
     2101 tccgggcatg cggcggatcg accgtgaaga tcagaaccgc aagctgaggc ctccgtacaa
     2161 caggtcctgc gctgcgggcc cgccctgctc ccggtatgcc gacaccacga gcagccacga
     2221 gcgcgccagc gtgtagtcga gatcggacga cacccagcgc accgtcgcgg tcgtacccgg
     2281 gacggcgctt cgctcgctcc gccagccgcc ggcgacctgc acgctgccgc gtccgccggg
     2341 ctccatcccc accgtgaacg cctgcagcca gccccggcgt ccggcggcgg cactggagta
     2401 ccgcgtgctg cgcgagcgca ggctcaggcc ccaacccgtc agccggtcgg ccccgatcga
     2461 cagcgtggtg gaacccgccg atccgatcgt gctcccgacc gaggtgcgcg cgtcgacgcc
     2521 gacctggaac cggcccgctc gagccgacag tcccgcccag gcgccgcgcc ggtaggtgtc
     2581 gtcgaacacg gtctccggat ttatcacatc gcggtacagc cggacgttac gccggttgtc
     2641 gaggccggcc gtcaacctta ccacgtcgct cggtcggtac accaggctcg cgaaggtact
     2701 ggtcggcgac agggcgtgct cccgcgctac ccggcgccac ggccggtagt agtccacttc
     2761 ctgggccgca tacagggaga ggcgccgggt ggcatagtcc ccctggagat agaggaactc
     2821 ccggttcgtg ccgctgtgca ggtaggagcc ggaaagacca gccgacagtc cccaacgcgc
     2881 gccggagccg ggccggctat ggcgttggag ataaccaccc agttgagtga cggtgccgga
     2941 caacgcgaga tcgatcgggt cgggctcgct ccccgcgaac agtccgccgc tccagcccgg
     3001 ccggtcgagt tgcgccgaca ccccgtccac gaggcccaca ggcgcgatgc cgggagcgta
     3061 ttggcgcccc accgtgagtc gcaacggcga cgccggtgcc tgccagctca gcgacgcctg
     3121 gtacaggcgt gcctgcgcct gcgcgtcggt gccgaacccg tcggcccggg tctgcacggt
     3181 gcgacggccg cgcgcgtcca ccgcgatccc cagtccggtc ccccccagac ggcccccgaa
     3241 cagccgcagg tcgcccgacg gttgtgtgaa gcgggcaccg ctgccgtcct gcgcctggat
     3301 cagcaggtaa tagaggccgg cccggccgcg cagtaccccg ccggcgcgtg gccgcgacga
     3361 agcgcgcccg tccggagcgc gccgcgtcac cgcgacggcg gagtcgggct ccgccgcagc
     3421 gggcgtgtat cgcaccgagt cgcccacggc gagtgaggcg gcgctgccgc tgacgtcgca
     3481 cgcggcctgg tgggtagcca gaaatgtgac cgcgaggacg gcgatggtgc tgtcgccgcg
     3541 gaccacctcc acacggctgt tgtccctcag tccttccgcc cgtccggcgt cgatgtaggc
     3601 tgacgccgtg gtcaggtagg tcacgtgcgc ccaacgcatc gccttcgcag gcacgtgctg
     3661 gccgctcacg cgggccggca ggatcaagac gccgcacaga aacgcgaggc gacgcatgct
     3721 cagccttcgc cccgtccgtt ccggtgacag ctgtagcact gctggctggt atagccgcgc
     3781 ccctggtgcg cattctggtg gcagtgcacg caatcgaact gccgatagtc gttcgcgttg
     3841 atgtggcact cggagcacga cgtccagcga ccctgatgcg cccccgagta gatcgggaag
     3901 aacgtccggt cgtggtcgaa acgagccccg gtccaccgcg ccgtcgtatg gcacccgctg
     3961 cagtccgtgg ggaaccccgc ggccacgtgg ttgggatccg tcgtcgcgtc gtagtcggtc
     4021 tggtggcacg acacgcaggc ggatggcagg gcctggaact ggcccacacc gtggcagcgg
     4081 gagcaattga tccgcagatg ggcgccggtc agcgcgaacc ccgtcgagcc gtgatcgaag
     4141 ttcgtccgca accaggtgat cgccgcgtgg cactgctggc aatcggtcgt gtagccgacg
     4201 gcggtgtgat cgggatcccg ggtggcccgg tagtccttga ggtggcaggt cgagcactcc
     4261 gtggcgagcg tgacgaaccg caaccgcccc tgcggcgcgg cgtggcacgc ttcgcaatcc
     4321 accgtcaggt gcgttccctg gagcgggaac cgggtcagct ggtgcgcgcg ggccatcccc
     4381 gaccgatcca ggaagttgcg cgcggtgtgg cagcgcgcgc agtcgttccc tacctccccg
     4441 cgatggggat cggcgtggca ggaaacgcag ctccgggagg tcccgcggaa gtcgagggtc
     4501 gtgtggcagg cccggcatgc gacggccgcg tgggcgccga cgagggcgag cccggccgtc
     4561 ttgccgtggt cgaaagccgc gctgacgtgc gccggcgtcc atccctccgg gctgtggcac
     4621 acgttgcacg acacgctcag ctcgccgtgc gggttcgcga ccaagcgatt ctgggcccgg
     4681 gcaggaccgg cgacgaccag gagcagtccg cccgccagcc cgaggactcg ggttgtcacc
     4741 atggcgtggc ggctccgagg caagttccgt actgtcgcgg gagagcgctg caagggccct
     4801 cgcaccaatc ggttcggcat cgggcccagt accacgcggg ctggggcgct gggtcgaaac
     4861 gattcagctg gcgcgtcgcg caacacggcg ggtgccgccg atcttcatga agcacggcga
     4921 tcagaatcgg taactcatcc ccgcgtacaa cagatcgtgc gcctccaggc caccggtctc
     4981 gcggtacgcg gaaaggacga cgaacagcga gcgggcgagg ctgacatcca tgtcggcgga
     5041 aagccagcgg atgtcgagcg ccggcgcggc ggtgcggtcg tgctcggtgc gccagccgga
     5101 cgtcaacgcc acgctgcctc gcccccatgg ctccaggccg aacgaaaccg agctcaacca
     5161 cccatcccgg ggcccggtag tgtagcgcgt gctgcgcgtg cgcaggccga tgcccagcga
     5221 cgtcagtcgt tcgacggcga gcgccagcgt gaaggtgttc gccgtgccgg tcgtcgcatc
     5281 gtgattggtg cgcacgtcga acgacgcacg gaagtgtcgc ggggtacgca ccgtcagccc
     5341 ggtccacacg ccgcggcgga aggtctcgtc gaacaccgcg gccgccgtgc tgtcgatgac
     5401 gtcgtggtac agccggacgc tgcgccgatt gtcgaatccc gcggtcaggc ccagcccgcc
     5461 cgtgacctgc agctggagat tggcgaagct gctcgtcggg gaaaccgcct gttcaccctg
     5521 gacgcggcgc catgggcgat aatagtcgac ttcctgcact ccatagaagg agaaccgtcg
     5581 cgtcaggtag ctcgcctgca ggtacaggaa ctcccggttc gtatgccagg tcacgtagga
     5641 cccgctcgcg ccggccgtga gggaccagtg cgtgagcgaa ccggcgccat tgtgacgccg
     5701 cacgtacccg cccagctggg agatatcgcc cgagaagccg aggtcgatca gctcgggctg
     5761 gagaccgccg aacaggccgt agtcccagcc ggattggttg atctctaccg ccgcgccgtc
     5821 gagcagccct acggaagtga tccccggggc atactggcgg ccggtcgtga agcgcaccgg
     5881 agaccccggc gcttgccagt acacctgcgc ctgatagacg cgggtctgat ccgtcgtgct
     5941 cgacctcgtg ccgggcaaca cccgaaccga ccgccgcgtg cgcacgtcca gcgcgagccc
     6001 caggggcgat ccgccaagtc cgcctccgct caaccgcagg tcgccggagg gctgggcgaa
     6061 gtgcccgccg aggctgtctc gctgcgcgac ggtgaggtag tacatcccga ttcggccgcg
     6121 cagcgctccc gtgctcgagc ggcgcgcgga gcgctgcggc gccggacgcg ccgccgattg
     6181 ggtcggctcg gcaaccgccg gaggaggagc cgccgtgctc gccgccggca gatagcgtgc
     6241 cgagtcgcct accatcacgc tgtccgccgc cgctacgatc tggcacgagg cctggtgggt
     6301 ggaaacgaac gacaccttca gctccccgac cgtacggcca gcgcggacga cgtcgacgcg
     6361 tgccccctcg atcaacccat ccgccttccc cgcgctgagg taggccgagc tcgtggtcac
     6421 gaaggtgatg cgcgccaggg gtgcacccgc tgtcggcacg gcgggcccta ccggcgcggg
     6481 cgccggtgca ggcgctcctg ctgcgggcgc ggcaggcggt ggcgcggccg ctgtggccgc
     6541 tgcgggggga ggcgcggctg gagccgtcgc cggggcgggc ggcgcgccgg aagccgttgt
     6601 gggaatggga accggacttc gggccggggc ggcgaccgcg ccccgagctc gcagacgggc
     6661 ggccgtgtca cgggccacgg gtgcattggc aaacaggaac cggacgtaat cgcccactcg
     6721 cagcggcacc gggcggctcg tgatcgtggc cgtggcctcg tgcgccgaca gggacgagac
     6781 cttgagcacc gcgacggccc ggccgctgcg gatgatctcc aatcgcgtcc acggtcgcac
     6841 gccatcggcg ctgcccacgt cgagcccgac ggcgccctcg gccacggagc gcacgcgccc
     6901 gaacttgtag ccgctgccga cctgctggct ccacgccgga gcggccagca gcagcgtgcc
     6961 aacgagcgat ccccactcga gacccgtgcg catcagtggg ttcctctcgg atggcaacgg
     7021 tagcaggcct ggctgtcgta ggtgtagccg ctgacgccgc tgtggtgcga gtcggtctgc
     7081 gtcttcccgt ggcatgacag gcagtcgaac tgccggtaat cgctcgagtt gatgtgacac
     7141 gtcgagcaac tggtccaacg cccggcgtgc gcgcccgagt agatcgggaa gtactgggcg
     7201 tcgtggctgg tgtaggtcgc cgtcgtccag accgtcgtcg cgtggcacgc cacgcagtcg
     7261 gtcgagaact gcgcctgtgc gtggttcgga tccgtcgtgc tgttgtaatc ggtctgatgg
     7321 cacgacacgc atgccgtcga cttccccttg tacacgccgt ccccgtggca ctggtcgcac
     7381 gtcgcctgca gatgcgcgcc ggtcagcgga aacgccgtgg cgttgtggtt gaaggtggcg
     7441 ccggtccagg tggtggtgga gtggcacgcg gtgcatacgg tcgggaactg cgactgcacg
     7501 tggttcgggt tcgtggcatt gttgaagtcg gtctggtggc acgacacgca cgctgtggac
     7561 ttgcccttgt acacaccgtc cccgtggcac tggtcgcacg tcgcctgcag gtgcgcgccg
     7621 gtcagcggga atgcggtcgc gctgtggttg aaggtcgcgc ccgtccacgt ggtggtcgtg
     7681 tgacaggcgg tgcagtcggt cgggaactgc gcctgcgggt ggttcgggtt cgtagcgttg
     7741 ttgtaggcgg tctggtgaca cgacacgcac gacgtgggct tgcctttgta cacgccgtcc
     7801 ccgtggcact gatcgcaggt gagctgcacg tgcgcgccgg tgagcgggaa ggccgtgctg
     7861 ttgtggttga aggtcgcccc ggtccaggtg accgtggtgt ggcacgcggt gcagtcggtc
     7921 gggaactgcg cctgcggatg gttcggattc gcggcgttgt tgtaggcggt ctggtgacac
     7981 gacacgcacg tggtgggcag agcgccgtac cgtccggtcc cgtgacactg ggcgcagttg
     8041 acggcgaggt gcgcgcccgt cagcgggaac ccgctccggc tgtgatcgaa acgcgccgac
     8101 tgccaggcga ccgtcgcgtg gcactgctcg cagctctgcg agaagccccc gcccgcatgg
     8161 tccggcgact tcgcggtctg gtagtccctg aggtggcaat cgttgcaggc cgtggatcgg
     8221 ctcgtgaatg tcaggtggcc ctgtgtcgag cgcgtgtggc agctctcgca gtcggcggtt
     8281 acgtgcgcac cggtgagcgg gaatcggctg agctggtgcg cccgcgtcat gccggtgcgg
     8341 tcgacgaagc tgcgcatcgt gtgacagcgc gcgcaggagc tgccgacttc accctgatgc
     8401 gggtctttgt ggcagctggc gcaatcgcgg gaggcgccct tgaacgtcag cgtcgtgtga
     8461 cacgacttgc aggcgacgct ggcatgggcg ccctcgtgcg ggaagccgga ctgcccgtgg
     8521 tcgaaggacg cgctgatctt cgccggcacc cagccggcgg cgctgtggca ggtcgcgcag
     8581 ggctgctcga gccgcccgtg cggattcctc gtatcggggc gttgggcgct cgccgcccgg
     8641 accgtcccga aaggcagcac gccccagagg atcgtcgctc gcagggtcat tgacggactc
     8701 catcaccgtg gcaggactca cagcgggtgg aaacgggacg ataccggatg ttgcgggtgt
     8761 taccgggacc ggcgatcgtg gcgtgacacc tggcgcacgc cacgttggca tggccgccgc
     8821 gcagcggaaa cttcgtgtcg cgatcatggt cgaaccgcga cgccggtgcg aatgcgtctg
     8881 caccgtggca gctctcacat ttgtcgccgc cctgacggcc ggcgaactga tcgccgtgcg
     8941 ggctcaggtg gcaccccaca cagccctgcg tccgcgtggt gaacagcagc ggcgatccgg
     9001 tccagcgggc ctggatcagc gtggacgccg ccggcaggta cttcagatcc gcgtggcacc
     9061 ccacgcacgg caccgcgcgg tgggcgccct cgagcttgaa ggcgtaggtg ccgtgcgcgg
     9121 ccacgtcgat cgtggagggc cggtagcgcc gggtgtcgtg gcagccgagg cagccgcccc
     9181 ggatcgggtg agccccccgc tcggcgaagc gcccctggtg cgggtccacg tggcaagcca
     9241 cgcattccag ctccccgagc cggaacaaca cgcctgcctt gccgacggtc ggcgtccgag
     9301 ggaacggcgc gagtcccttg cgctgggtgc tgtgacaggc gtcgcacccg gcctcggcgt
     9361 gtgcgccctc gagccggaac cgcgccttcg cgtgagcggc gaccgtgaag gtgctgggct
     9421 tccagcccgc gaccgagtga caggcggtgc agtcgccgcc gtccgcccgg gcacccagct
     9481 ggccgccgtg gtcatcccgg tggcaatcga ggcagcgcgc gaacgccggg tgcatctgga
     9541 tgccggccgt gccgagccgc ccggccggca ctcccgccgg attcttcacg tggcacgaga
     9601 cgcaggccac ctgacgatgc cgtccctcga gcgggtactt cgtcttcgcg tgctgctcga
     9661 gcgtgaaccc ggcgaccgcg aatcccgcca ccgtgtggca cgcggcgcaa tccaccaccg
     9721 cgccggcgag ggtcgccgtc ccgccgtggg gatcggcgtg gcacgacgta caggtagcga
     9781 acggcgggtt cttgcccttg agacccggcg tgtcgtggca cttggcgcaa gtcacggaca
     9841 catgctcacc gcgcagcggg tagcgggtcc ggtcgtgatt gaagttgccc cggtcgatga
     9901 ccttgaaccc ggtggtcacg tggcagtcgc tgcaggggcc tgcgaaacgg ccgcggtgcg
     9961 ggtccgtgtg acaggcggcg cagtcgcggt gcgccaccgg cttgaagacc gcgaccgtgt
    10021 cgccggccgc cggcttgtgg cacgacgcgc aacgcacatc acggtgcttg ccggtcagcg
    10081 gatagtcgga tctcgcgtgg tcgaacttcg gcgccggcga ccagcgctcc gtgtcgtggc
    10141 acgagacgca atcctgtccc aacgagcgcc ggtgtacgtc gcggtggcac gacgtgcagg
    10201 tcgtttccag cccgacccac cccggcgccg ccgacggctg gggcgagagc gcggccgccg
    10261 gtccggtgcg gaacgccgcc ttgtggcagt cggcgcacgt gatcgtcgcg tggcgcccct
    10321 ccagcggcca tcccgcgagc gcgtggtcga accgcgcgac atcgcgctcc ggccaggcga
    10381 tcagggcgaa tgctcttcct gcatggtctg ggtgacagct cgagcagggc tgctgctgca
    10441 cccgggcgtg gtagccgcga ttctgctgcc gcagggaggc gatctccttg tggcactcga
    10501 ggcagcgtac cgtcatctgt tccttgctgc cgccgccgtg gcaattgacg cagcgcagcg
    10561 ccccttcgag ctgcacatgc ggcttggcga gcggtcccgg ggagatctgg gcgtgcaggg
    10621 gtgcgcccag caagagcgcc gacacccaga ccaatccgac ggatgtgccg acacccttca
    10681 ccacaaccac gtcgcgccga gcgccacgac gacggtcacg tggaccagga ccgccagcag
    10741 ggcggcgatg gcgaccggcc ggtgcagcac gtgccaccag cggaacaacg cgtgggtagc
    10801 gtccagcatc cggccttgct gggtcagcgc catctcccgc ttcgcaagcc gcaacaccca
    10861 ccggaacatc tgccgatcct ggttatggcg gcgcaggccg ctctgcgcgt acagccggcg
    10921 gaagcgccgc gccgcccggc ggcgcgccag atcatccgcc agcagctgga acagggtccg
    10981 cacgaccccg gaccgcccac tggggccccg cccggtgtcg agcgccgcct cgatgcgctg
    11041 cagatcgagt cccgtactcg tcgcgatctt ctcgagcagc tgcctgcgcc gcgcgccgat
    11101 ctcttcgagc gtcagctcga tcccggcctt gccgcgtggg atgcgcgtat acagataccg
    11161 gccaaccaca ccgctcgccc acaccaccat catggaccag aagcccagcc cgatcaggcc
    11221 ttcgaagcgc caggccgcat ggatcgcgac gagcagcggc agcgacagcg ccgcgagcac
    11281 gtgcacgtcc agccagcggc ccaccgatcc cgtccacgcg agccagcgag ccttcttgcg
    11341 aaacggatac agccacagaa agatgaagat cgcgatcgcc agcaacccgg cgctctgccc
    11401 cacataccct gccggcttga gccagggatg gaaggggctg cgtacccgct ccgccaccgg
    11461 ctgggtatag tagggcaggc cgaccacgca gatcgcgacg agcagtccga cgaaggtcag
    11521 cagcagaaaa cgggacgccc ggataggact ccgcgacacc tcgccactgg ggcgggacac
    11581 ccggggactc gaaatcgggg aaggtctcgg ggggacggcg cgcggacgtg gagcggcggc
    11641 gctcgactcg gatctgacgc tcactaggcc tccccggcgc tcgccgggta g
//
LOCUS       DBNGZW010000215         4361 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_422898, whole genome shotgun sequence.
ACCESSION   DBNGZW010000215 DBNGZW010000000
VERSION     DBNGZW010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4361)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4361)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4361
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_422898"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1677
                     /locus_tag="AC16JC_05190"
     CDS             <1..1677
                     /locus_tag="AC16JC_05190"
                     /inference="COORDINATES: protein motif:HMM:NF051835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HIR5164127.1"
                     /translation="TPPAVAIVEITPPTASLQPGAHAQLSVRARDSHDRPISDRRVSW
                     SSSDQTIATVTPQGSVTAVKVVRATITAQIETHRASAAVTVTAVPVANAALSPASLTL
                     SVGRSALLAVSLRDARGGALRNRDVRWATGNPAVATVSSTGSVTAVGAGTTTITATSE
                     GVTASATVTVSAPAVAVNPPPPPTQPAESQPPAAQPAPSPPTEGASRLLPRRGIAAGG
                     SHSCGITQEGAAVCWGSNASGQLGGASTGAAASAPVQVSPGTFTSLVAGTDHTCGLTQ
                     SGAAICWGLNTRGQLGSGHTAGSPAPGAVAGGHVFVALAAGARHTCGLTSDGTAWCWG
                     DNGTGQLGDGSTRGANLPRRVVGDTKFRALAAGSDHTCGLATDGRVFCWGDGFSGQLG
                     RGARETMSEPVPVDAGVKFTVLTAGGKHSCALATGGKAYCWGANVAGEIGDGSKSERD
                     RPVAVSGTRVFTMITAGPEHTCALTEAGDSFCWGRNRDGQLGDGSRVDRTAPVAVALD
                     RPLASLTAGGSHTCGVTRSQTVLCWGGNVRGQLGDGTTTARPSPAPVEAH"
     gene            1714..2406
                     /locus_tag="AC16JC_05195"
     CDS             1714..2406
                     /locus_tag="AC16JC_05195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164128.1"
                     /translation="MSTTKRIGRGLTLAGILAGAAAVAVPNALHAQGKIELTPFLGSY
                     YALTKMCTDCNKDGSDVTGHQVNAFAFGGRLSYWVSNTIGVEGAFTYAPSRAEERVGI
                     SGFGLAVSGKGRIMQASGRVLYRPQRTNLHFIVGAGIVSRGDTVWQAFKENAPDTVGA
                     KLTSVAGILGVGVRASVTPKFALNLAAEVNLYSFDPRLATVTSPAPNSANGGKLQADL
                     LVSVGIPITLSR"
     gene            2523..3020
                     /locus_tag="AC16JC_05200"
     CDS             2523..3020
                     /locus_tag="AC16JC_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164129.1"
                     /translation="MFRPVAVLVIAAALSAPPRVAGQRLAPAYLGADLPTTSDSRPGA
                     AGSRDGAMVLGGLAGGVVGFFAGGYTGAMAGGGNRVCGDDPCGLEEAIWGAAAGVSTF
                     IPLGVHLANGRRGRYGAELAASLVVGAVGLGLAYNSNTGVPLLLVPVGQIVTSMLIER
                     AAARH"
     gene            complement(3038..3111)
                     /locus_tag="AC16JC_05205"
     tRNA            complement(3038..3111)
                     /locus_tag="AC16JC_05205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3075..3077),aa:Arg,seq:tct)
     gene            complement(3228..3638)
                     /locus_tag="AC16JC_05210"
     CDS             complement(3228..3638)
                     /locus_tag="AC16JC_05210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164130.1"
                     /translation="MMTSRFPRAVAAVVLTTIAVLAACSDSTGPLAPFQPQISNVADN
                     FQFQATGVTNATWTYTYTWPNSGDSATVNQATTVTAGSATLIVFDKNGTQLYSQSLSA
                     NGTFGMLKGVAGSWTVRVVFTGYSGTVNFRVQKA"
     gene            complement(3883..>4361)
                     /locus_tag="AC16JC_05215"
     CDS             complement(3883..>4361)
                     /locus_tag="AC16JC_05215"
                     /inference="COORDINATES: protein motif:HMM:NF016668.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HIR5164131.1"
                     /translation="SAQVERALRSGLKIDYVDYHMGTAASTPQLLAVVERIADKYGLG
                     ISRYFGEVYHTMFDTPVAEKQSAFHAYLDSLTPGPLNLVVIHVAQSTPEIRALVDMNN
                     AAQNTAMGEPLMAQHRQAELDMLLQFARGPRKVSLVTYSGVIARLGRASMRRPASQ"
BASE COUNT          695 a         1472 c         1532 g          662 t
ORIGIN      
        1 acaccgcccg ccgtcgcgat cgtggagatc actccgccca cggcctcgct gcagcccggc
       61 gcccacgcgc agctttccgt gcgcgcgcgg gattcgcacg accggccgat cagcgaccgc
      121 agggtgagct ggagctcgag cgatcagacc atcgcgacgg ttacccccca gggctcggtc
      181 accgcggtca aggtggttcg ggcgaccatc acggcgcaga tcgaaaccca ccgcgcctcc
      241 gccgccgtca cggtgaccgc cgtgccggtg gcgaacgccg cgctgtcgcc cgcctccctg
      301 acgctgtccg tcggccgatc cgcgctgctc gcggtgagct tgcgcgacgc gcgcggcggc
      361 gccttgcgca accgggacgt gcggtgggcg acgggcaacc cggcggtggc caccgtgtcg
      421 tcgaccggat cggtcaccgc ggtcggcgcg gggacgacga ccatcaccgc gacgagcgaa
      481 ggcgtcaccg cgtccgcgac ggtcaccgtg tccgcgccgg ccgtcgccgt gaatccaccg
      541 cccccgccga cgcagccggc ggaatcgcag ccgccggcgg cgcagccggc tccctcgccc
      601 cccaccgagg gggcgtcgcg cctgctgcct cgccgcggca tcgcggcggg cggctcccac
      661 tcgtgcggga tcacccagga aggcgccgca gtgtgctggg gcagcaacgc cagcggccaa
      721 ttgggaggcg cgtccaccgg cgcggcggcg agcgcaccgg ttcaggtgag tccaggtacc
      781 ttcacctccc tcgtcgccgg caccgaccac acttgcggac tgacgcaaag cggcgcggcg
      841 atctgctggg gcctgaacac ccgggggcag ctcggcagcg gacacaccgc cgggtcgccc
      901 gctcccgggg ccgtggccgg cggtcacgtg ttcgtcgccc tcgcggccgg cgcccggcat
      961 acgtgcggcc tgacgtcgga cgggaccgcg tggtgctggg gcgacaacgg caccgggcag
     1021 ttgggggacg ggagcacccg gggcgccaac ctgccccgcc gcgtcgtggg cgacacgaag
     1081 ttcagggcgt tggcggccgg ttccgaccac acctgcggcc tcgcgaccga cggccgggtg
     1141 ttctgctggg gcgatggatt ctcgggtcag ctgggacgtg gcgcacgcga gacgatgtcg
     1201 gaacccgtcc cggtcgacgc cggcgtgaag ttcaccgtgc tgacggcggg cggtaagcac
     1261 tcgtgtgccc tcgccacagg gggaaaggcg tattgctggg gggcgaacgt cgcgggcgag
     1321 atcggcgacg gcagcaagag cgagcgcgac cgccccgtgg ccgtgtcggg cacacgggtg
     1381 ttcaccatga tcacggcggg cccggagcac acgtgcgcgt tgaccgaggc gggcgatagc
     1441 ttctgctggg ggcggaaccg ggacggccaa ctgggcgacg gatcgcgggt agaccggacg
     1501 gcccccgtcg cggtcgcgct ggaccgcccg ctcgcgtcgc tgaccgccgg gggctcccac
     1561 acctgcggcg tcacgcgttc ccaaaccgtc ctgtgctggg gcggcaacgt ccgcggtcag
     1621 ctcggcgacg gcacgaccac ggcccggccg agcccggcgc cggtcgaagc gcattagcgt
     1681 atgtcaccgt tcattccaca caggggagat accgtgtcaa cgaccaaacg gatcggcagg
     1741 gggctgacgc tcgcgggaat cctggcaggc gccgccgccg tcgcggtccc gaatgcgctg
     1801 cacgcgcagg ggaagatcga actgaccccg ttcctgggca gctactacgc cctcacgaag
     1861 atgtgcacgg actgcaacaa ggacggcagc gatgtaacgg gacaccaggt gaacgccttc
     1921 gcgttcggcg gacgtctgag ctactgggtg tccaacacca tcggggtcga aggcgccttc
     1981 acctacgcgc cgagtcgcgc ggaagaacgg gtgggcatct ccgggttcgg cctggcggtc
     2041 tctggcaagg ggcggatcat gcaggcgagc ggccgggtgc tctaccggcc acagcgtacc
     2101 aacctccact tcatcgtggg agccgggatc gtcagccgag gcgacacggt ctggcaggcc
     2161 ttcaaggaga atgcgcccga tacggtcggc gcgaagctca cgtcggtggc tggcatcctg
     2221 ggggtgggag tgcgcgcgtc ggtcacgccg aaattcgcgc tcaacctggc ggcagaggtc
     2281 aatctctact cctttgaccc gcggctcgcc acggtcactt cgccggcacc gaattcggcc
     2341 aacggtggga agctgcaggc ggatctgctg gtctcagtgg gtattccgat caccctgagt
     2401 cgctagcctg cttcacacgt tctcgcgtac gggccccggc acacgccggg gcccgtcgtg
     2461 cttgaagacg gcttgagggc ggcgccgaac ttctcctgca gacaccgatc gaggagaccg
     2521 ccgtgttccg ccccgtcgcc gtgctcgtga tcgccgccgc tctgtcggct ccgccgcgcg
     2581 tggctggcca gcgcctggcc cccgcatacc tcggcgcgga cctgcccaca acgtccgatt
     2641 cgagacctgg cgcggccgga tcgcgcgacg gcgccatggt gctgggcggt ctggcgggcg
     2701 gggtcgtcgg attcttcgcc gggggctaca ccggtgcgat ggccgggggg ggaaatcgag
     2761 tctgcggcga cgatccctgt gggctcgaag aggcaatctg gggcgccgcg gccggggtga
     2821 gcaccttcat tcccctcggc gtccacctcg cgaatggccg gcgcggccgc tacggagccg
     2881 agcttgcggc cagtctggtc gtgggagccg tcgggttggg actcgcctac aacagcaaca
     2941 ccggcgtccc cctgctcctc gtaccggtag gacagatcgt gacaagcatg ctgatcgagc
     3001 gcgcggccgc ccggcactag cggcggccat cggaggacgc gcccggcagg attcgaacct
     3061 gcgaccctcg gcttagaagg ccgatgctct gtccagctga gctacgggcg cccgttgccg
     3121 agaggtattg tagtgaagtg ggacgatgga tgccatgagt caaggagcga agggcaggcc
     3181 cggtatgaaa ccggacctgc ccttggggaa cgaacgctcc aacccttcta ggccttctgg
     3241 acccggaaat tcacggtacc gctatagccc gtgaacacca ccctgaccgt ccaacttccg
     3301 gctacgccct tgagcatccc gaaggtgcca ttggcgctca acgactggga gtacagctgc
     3361 gtgccgttct tgtcgaacac aatgagtgtg gccgagccag cggtgaccgt ggtcgcctgg
     3421 ttcacggtgg ctgagtcccc ggagttcggc caggtatagg tgtaggtcca cgtggcattc
     3481 gtaaccccag tggcctggaa ctggaaattg tcggccacat tgctgatctg aggctggaat
     3541 ggcgccagcg gaccggtgct gtcgctgcac gcggcgagaa ccgcaatggt cgtcagaacg
     3601 acagcagcga cagcgcgtgg aaatcgactc gtcatcatgc gtacaagctc ccgaggaact
     3661 caatggtgga tggaacagga tcgagaccgg gtgccttggg aagcaagctg gaggccagtt
     3721 cgggacgtgc ggcgaactca cacggccctt ttgagttacg cgcagatgtg tgatggatgg
     3781 ccttcgccgc gcctagaccg attccgcagt ccatgtctca tttcgataca gcagaaccaa
     3841 gagggtagtc gcgtcgccgg gagtgggcga ccttcgctcc cgctactgcg aggcaggccg
     3901 ccgcatgctc gcgcgtccca gccgggcaat gaccccggag taggtgacga gcgagacctt
     3961 tcgtgggccg cgcgcgaact gcaacagcat gtccagctcc gcctgccgat gctgggccat
     4021 caggggctca cccattgccg tgttctgcgc cgcgttgttc atgtcgacca gggcgcggat
     4081 ctccggagtg gattgcgcga cgtgaatcac caccaggttg agcggtccgg gagtcaggct
     4141 gtcgagatag gcgtgaaacg ccgactgctt ctccgcgacg ggcgtgtcga acatcgtgtg
     4201 atagacctcg ccgaaatagc gcgagatgcc gaggccgtac ttgtccgcaa tgcgctccac
     4261 cacggccagc agctgcggcg tcgacgcggc cgtgcccatg tggtaatcga cgtaatcgat
     4321 cttcaggccg gaccgcagcg cccgctcgac ctgcgccgac a
//
LOCUS       DBNGZW010000216         2543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1450966, whole genome shotgun sequence.
ACCESSION   DBNGZW010000216 DBNGZW010000000
VERSION     DBNGZW010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2543
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1450966"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2494
                     /locus_tag="AC16JC_05220"
     CDS             <1..2494
                     /locus_tag="AC16JC_05220"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011140762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR5164132.1"
                     /translation="LAEGLTDDQAHTLAREGKAVVWIDGGLHATEVLGAHQLMELVYQ
                     MLSRGDAETRRFLDDVILLAVHANPDGMELVSSWYLREPDTLKRATSTIPRLYQKYIG
                     HDDNRDFYMVTQPETENMARVMFCEWFPQIVYNHHQTGPVGTVLFAPPFRDPFNYNYD
                     PLIPLGVEMVGTAMHARLAAEGKPGATMRSGSSYSTWWNGGLRTTAYFHNMIGLLTET
                     IGNPTPVDIPFIPDRELPRGDLAYPVEPQRWHFRQSIDYSISMNRAVLDYASRYRETL
                     LYDIYLMGKHAIERGSHDSWTIHPQLIEAVKQAARDSARSDTTRPPQTGPAGSVPTKL
                     FALLHDPDRRDPRGYILPADQPDFPTATKFVNTLIKNGVTVLRATREFEVAGKRYPAE
                     SYVVKTAQAFRPHILDMFEPQDHPNDFQYPGGPPIPPYDNAGWTLAYQMGVQFDRVLD
                     GFDGPFARIEGFATPPPGALANADGAAGFLLDHRVNDVVILTNRLLAQQQAVYWLTRP
                     LAVDGKTYPAGTVYVPATPASLQLLRRGAAELGLRGDGIAAAPPGDALRLRPARIALW
                     DRYGGSIPSGWTRWLLERFEFPFAVVYPAALDTGKLAARFDVLVLPDGALGDSGATGG
                     GGGGVGAAPDSLRLPPEYRAWLGRLTVAKTVPQLRQFLDDGGTIIALGGSTGLARYLG
                     LPISNHLVQDGKPLPRTAFYVPGSVLQVRVDTVRPVAWGSAERVDVFFDESPVFDLSP
                     DAFGAGVRPVAWFDSARPLRSGWAWRQERLEHVVAVAEAAVGAGSGRLYLFGPEILNR
                     GQSHGTFRFFFNAIQLTGATAGGAAAASGEAH"
BASE COUNT          384 a          903 c          865 g          391 t
ORIGIN      
        1 cctggccgag gggctcacgg acgaccaggc ccacacgctg gcgcgcgagg gcaaggcggt
       61 ggtgtggatc gacggcggcc tgcacgccac cgaggtgctg ggcgcgcatc agctcatgga
      121 gctcgtctac cagatgctga gccggggcga cgcggagacc cggcgcttcc tggacgacgt
      181 gatcctgctc gcggtgcacg cgaatcccga cggcatggag ctcgtgtcct cgtggtacct
      241 gcgggagccc gacaccctga agcgcgcgac gtccaccatc ccccgcctgt atcagaagta
      301 catcgggcac gacgacaacc gcgacttcta catggtcacg caaccggaaa ccgagaacat
      361 ggcgcgcgtc atgttctgcg agtggtttcc ccagatcgtc tacaaccatc atcagacggg
      421 gccggtgggg acggtcttgt tcgcgccgcc gttccgcgac ccgttcaact acaactatga
      481 tcccctcatc ccgctcggcg tggagatggt cgggaccgcg atgcacgcgc gactcgccgc
      541 cgaggggaag ccgggcgcca ccatgcgctc ggggtcgagt tactcgacgt ggtggaacgg
      601 cgggctgcgc accaccgcgt acttccacaa catgatcggc ctgctgaccg agacgatcgg
      661 caaccccacc cccgtcgaca tcccgttcat cccggatcgc gagcttccca ggggggacct
      721 cgcgtacccg gtggagcccc agcgctggca cttccggcag tcgatcgact attcaatctc
      781 catgaaccgg gccgtgctcg actacgcgtc ccgctatcgt gagacgctgc tctacgacat
      841 ctacctcatg gggaagcatg cgatcgagcg cggcagccac gacagctgga cgatccaccc
      901 ccagctgatc gaggccgtca agcaggcggc tcgggacagc gcgcgcagcg atacaacgcg
      961 cccaccacag accggacctg ccggctccgt gccgacgaag ctgttcgcgc tgctgcatga
     1021 tcccgaccgg cgcgacccgc gaggctacat cctgcctgcc gatcagcccg atttcccgac
     1081 cgcgacgaag ttcgtgaata cgctgatcaa gaacggcgtc accgtgctcc gggcgacccg
     1141 tgagttcgag gtggcaggga agcgctaccc cgcggagtcg tacgtcgtca agaccgctca
     1201 ggcgttccgg ccgcacatcc tcgacatgtt cgagccgcag gatcacccca acgatttcca
     1261 gtatccgggt ggcccgccga tcccgcccta cgacaacgcc ggctggacgc tggcctatca
     1321 gatgggcgtg cagttcgacc gggtgctcga cgggttcgac gggccgttcg cacggatcga
     1381 gggcttcgcg acgcccccac ccggcgcgct cgcgaatgcc gacggggccg ccggcttcct
     1441 gctcgatcac cgggtgaacg acgtcgtgat cctcacgaac cgtctgctcg cgcagcagca
     1501 ggccgtctac tggctcacgc ggccgctcgc tgtcgatggg aagacgtacc cggccggcac
     1561 ggtctacgtc cccgccacgc ccgccagcct tcagctgctg cgacgaggag cggcggagct
     1621 cggcctgcgc ggcgacggca tcgccgctgc gccccccggg gacgccctgc gcctacgtcc
     1681 ggcgcgcatc gccctgtggg accggtacgg cggatcgatc ccgtcgggct ggacgcgctg
     1741 gctgctcgag cggttcgagt tcccgttcgc ggtagtgtac ccggccgcgc tcgataccgg
     1801 gaagctcgcg gcgcggttcg acgtgctggt gctgccggac ggcgcgctcg gcgacagcgg
     1861 cgcgacgggc ggtgggggag ggggcgtcgg tgcggcgccc gactcgctgc gccttccccc
     1921 ggagtaccgc gcctggctgg gccgcctgac ggtcgcgaag acggtgccgc agctccgaca
     1981 gttcctcgac gacggcggga cgatcatcgc gctgggcggc tccaccggcc tggcgcgcta
     2041 cctcggtctc cccatctcca atcacctcgt gcaggacggg aaaccgctgc cccgcacggc
     2101 gttctacgta ccgggttccg tgttgcaggt ccgtgtggac accgttcgcc cggttgcgtg
     2161 gggatccgcg gagcgcgtgg atgtcttctt cgacgagagc ccggtcttcg acctctcgcc
     2221 cgacgcgttc ggggccggcg tgcggccggt ggcctggttc gactcggccc ggccgctgcg
     2281 cagcggctgg gcctggagac aggagcggct ggagcacgtg gtcgccgtgg ccgaggcggc
     2341 ggtgggcgcg gggagcggta ggctgtacct gttcggcccc gagatcctga atcgcggcca
     2401 gtcccacggg acgttccggt tcttcttcaa cgcgatccag ctcacgggcg cgacggcggg
     2461 cggggcggcg gcggcgagcg gcgaagcgca ctgaagggcg cctggacgct tcaccccaga
     2521 atccgatcga tccccgccag ctc
//
LOCUS       DBNGZW010000217         1812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_631815, whole genome shotgun sequence.
ACCESSION   DBNGZW010000217 DBNGZW010000000
VERSION     DBNGZW010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1812
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_631815"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1294)
                     /locus_tag="AC16JC_05225"
     CDS             complement(<1..1294)
                     /locus_tag="AC16JC_05225"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR5164133.1"
                     /translation="MTGGRSIGRKQPLPEPVYQEAFPRDPDFPQLGVATDPARMLEVF
                     RAHLQPVVSRSYAIRDCVPFRFRCRQSGGRCVLQYTLRLEEVRTGRQWEQWVTGLLYA
                     RSGEAERLWQELRAGEPVREIPLGWLSFQPVDFVPELEMLVQVFPFDRKLPQLGPLLS
                     GAVDELEPQLLDRLRPAGRWQVTRSTVVPTRYRTELGAALKLTLHAQETSSGRNATLN
                     CYLKAFRNNHGAETFAFLRDYAERPERDSTGFDVVRPLAYLKERRTLALEEAVGATLQ
                     QHLLHGREPAVAAKAVARAVAAFNQHDATIPLHHTLDDQLADVQRAGTLVQWACPESR
                     GAVRQIVAALEGGLEEVSPAPIHRDLKPDHVFLSGGRVRFIDCDSVALGDPARDPAHL
                     FAHIVGGVGLEAMPFAHRAGAAAAFVLEYFAHVPSYWRR"
     gene            complement(1291..>1812)
                     /locus_tag="AC16JC_05230"
     CDS             complement(1291..>1812)
                     /locus_tag="AC16JC_05230"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5164134.1"
                     /translation="QIGVVLQSSILFGSSVKENIAYGNPEATEEQIEAAARAANADEF
                     IRELPQGYDTVIGERGATLSGGQQQRIAIARALIRDAPILILDEPMTGLDVESEAKVR
                     EALDRLMTGKTCIMMTHDLQSIADAHQVLVLEGGQLVAQGAHADLLATSARYRELYEM
                     DRDQAQAVEEPSV"
BASE COUNT          269 a          635 c          624 g          284 t
ORIGIN      
        1 ctcggcgcca gtagctgggc acgtgagcga agtactccag gacgaaggcc gcagcggccc
       61 cggcgcggtg ggcgaagggc atggcctcca gaccgacccc gcccacgata tgggcaaaca
      121 gatgcgcggg gtcgcgggcg ggatcgccca gggcgaccga atcgcaatcg atgaaccgga
      181 cacggccgcc ggagaggaac acgtggtcgg gcttcaagtc ccgatggatc ggtgcgggac
      241 tgacctcctc cagccccccc tcgagcgccg cgacgatctg tcggacggcc ccgcgactct
      301 cggggcaggc ccattgcacc agcgttcccg cccgttgcac gtcggcaagc tgatcgtcga
      361 gggtgtggtg cagcgggatc gtcgcgtcgt gctggttgaa cgcggctacc gcgcgggcga
      421 cggccttggc cgccaccgcg ggctcccggc cgtgcaggag gtgctgttgc agcgtcgcgc
      481 cgaccgcttc ctccagggca agggtgcgcc gctccttcag gtaggcgagc gggcgaacca
      541 cgtcgaaccc cgtcgagtcc ctctcgggcc gctcggcgta gtcccgcagg aaggcgaacg
      601 tctctgcgcc gtggttgttg cggaaggcct tcagatagca gttcagcgtg gcgttccgcc
      661 cgctgctcgt ctcctgggca tgcagcgtga gcttgagcgc agcccccaac tccgtgcgat
      721 accgcgtcgg gacgaccgtg ctccgcgtga cctgccaccg tcccgccggg cgcaggcggt
      781 cgaggagctg cggctcgagc tcgtccaccg ctccgctgag cagcggaccc agctgcggca
      841 gtttgcggtc gaaggggaag acctgcacca gcatctcgag ctccgggacg aagtccacgg
      901 gctggaagct caaccagccc agcggtatct cccggaccgg ctcgccggcg cgcagctcct
      961 gccacagtcg ttcggcctcg cccgagcggg cgtacaggag tcctgtcacc cactgttccc
     1021 actggcggcc ggtacggact tcctcgagcc gcagcgtgta ctgcagcaca cagcgtccgc
     1081 ccgactggcg gcagcggaac cggaacggca cgcagtccct gatcgcgtag ctcctgctca
     1141 ccacgggctg caggtgcgcg cggaacacct cgagcatccg cgccggatcg gtggcgacgc
     1201 cgagctgcgg aaagtcgggg tccctgggaa acgcttcctg gtagaccggc tccggcagcg
     1261 gctgcttgcg cccgatggat cgcccgccgg tcatacgctc ggctcctcga ccgcctgggc
     1321 ctgatcccga tccatctcgt acagctcgcg gtagcgggcg ctggtggcca ggaggtcggc
     1381 atgcgcgccc tgagcgacga gctggccgcc ctccaggacc agcacctgat gggcgtcggc
     1441 gatcgactgc aggtcgtggg tcatcatgat gcaggtcttt ccggtcatca gacgatccag
     1501 ggcctcgcgc accttggctt cactctccac gtcgaggccg gtcatcggct cgtccagaat
     1561 cagaatgggg gcgtcgcgga tcagcgcgcg ggcgatggcg atgcgttgct gctggccgcc
     1621 cgacagggtc gcgccccgct ccccgattac ggtatcgtag ccctggggca gctcgcggat
     1681 gaactcgtcg gcgttggcgg cacgggccgc cgcttcgatc tgctcctcgg tcgcctccgg
     1741 gttgccgtac gcgatgttct ccttcaccga ggagccgaac agaatcgagc tctgcagcac
     1801 gacgccgatc tg
//
LOCUS       DBNGZW010000218         2735 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1519777, whole genome shotgun sequence.
ACCESSION   DBNGZW010000218 DBNGZW010000000
VERSION     DBNGZW010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2735)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2735)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2735
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1519777"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="AC16JC_05235"
     CDS             <1..1425
                     /locus_tag="AC16JC_05235"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HIR5164135.1"
                     /translation="PSAVGSACCDAVNRGAVYADGKIVFNVLDGHTVAVDAVTGAELW
                     KTQIADLGQGETTPMAPLVVKNLVFVGPSGGEFGIHGWLKALDLNTGRVVWTAYNEGP
                     DSEVRVTHGTFKPFYDRGADLGLSSWPGDAWKRAGVPVWGWLSYDPQLDLLYFGTGNA
                     SPYNPEQRAGDNKWASSVLARRPADGSLVWAYQFTPHENWDYDANAEMILVDLDVGGR
                     SRKVLVHFDKNGFAYTIDRVTGEVLVAQPFTPVTWAKSVDLATGRPVLDSTKLTGASR
                     GNITGICPSLEGGKSPVSAASYSPRTGLFYVSTNNLCMDYQAAQVTRIAGTPYIGAAT
                     PYKPGPGGYMGAFIAWDARTGRKVWEIRERYPVWSGSVATAGDVVFYGTLDGWFKAAD
                     ARTGNPLWKFKVGSGVVGCPITYRGPDGRQYVAVYAGIGGDWFLLSGDVRSDDPADVR
                     APADFMPDIARHTSQGGIVWIFAL"
     gene            1407..1853
                     /locus_tag="AC16JC_05240"
     CDS             1407..1853
                     /locus_tag="AC16JC_05240"
                     /inference="COORDINATES: protein motif:HMM:NF024834.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5164136.1"
                     /translation="MDLRAVAGLGLLAAAGCRGASREAGQAPPSIRYEQHVAAGGVAP
                     VAATLANPFAGDRASVAEGERTFVAMNCDGCHGGGATGWVGPSLVDGRWRYGGADGAV
                     FHSIYYGRARGMPAYGGVLSPDAIWKIVTYLRAQPLPADVPTERWQ"
     gene            1850..2470
                     /locus_tag="AC16JC_05245"
     CDS             1850..2470
                     /locus_tag="AC16JC_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5164137.1"
                     /translation="MTTSGRYGFLTETYATERLKVLSVWSMFEDGDLAVRPHHTDARG
                     RSVLEHMVHQSLSEDLWFRTMLGIDVGAPPLPDTETRTAFLRSYGKRSAQRLTALRAK
                     PDEWWEEEVAFFDVRRSRAWVMVRRIAHTAHHRGQQTALLRMLGRSLHSTYGPTADTG
                     GLFQHGAATVYAYGDEDALLKGEATGGRKAALPGPGAAPVTERPAP"
     gene            2467..>2735
                     /locus_tag="AC16JC_05250"
     CDS             2467..>2735
                     /locus_tag="AC16JC_05250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164138.1"
                     /translation="MMNADAANLPALMCRRSWEADDLVRRWPPKFAHPNKKVVYDVSC
                     PSGTSHEGTVTFSRWTGGRLATAVPGERTAEVLGRPGFFDYARPAA"
BASE COUNT          426 a          916 c          985 g          408 t
ORIGIN      
        1 ccttccgcgg tcggcagtgc ctgctgcgac gcggtgaacc gcggcgccgt ctatgcggac
       61 gggaagatcg tcttcaacgt gctcgacggg cacacggtcg ccgtcgacgc cgtcaccggg
      121 gcggagctct ggaagacgca gatcgccgac ctcggccagg gcgaaaccac gccgatggcg
      181 ccgctcgtgg tgaagaacct ggtcttcgtc gggccctcgg gcggcgagtt cggcatccac
      241 ggctggctca aggcactgga cctgaatacg ggccgggtcg tctggaccgc ctacaacgaa
      301 gggccggaca gcgaggtgcg ggtcacacac ggtacgttca agccgttcta cgaccggggc
      361 gccgacctgg gactctcgag ctggccggga gacgcctgga agcgcgcggg cgtgccggtg
      421 tggggatggc tgtcctacga tccgcagctc gatctcctct atttcggcac cgggaacgct
      481 tcgccctaca accccgagca acgcgccggc gacaacaagt gggcctcgag cgtactggcg
      541 cggcgccccg ccgacggctc gctggtctgg gcgtaccagt tcacgccgca cgaaaattgg
      601 gactacgacg ccaacgccga aatgatcctc gtggacttgg acgtcggcgg ccggtcgaga
      661 aaggtcctgg tgcacttcga caagaacggt tttgcctaca ccatcgatcg tgtcaccggg
      721 gaggtgctgg tggcccagcc gttcaccccg gtgacctggg cgaagagcgt ggacctcgcc
      781 accggccggc ccgtgctcga ctcgaccaag ctcaccgggg cctcgcgcgg caacatcacg
      841 gggatctgcc ccagcctgga aggcggcaag agccctgtgt ccgccgcatc ctactccccg
      901 cgcaccgggc tgttctacgt ctcgaccaac aacctctgca tggactatca ggcggcgcag
      961 gtcacccgta tcgccggcac gccctacatc ggcgccgcca cgccctacaa gcccgggccc
     1021 ggcgggtaca tgggcgcgtt catcgcctgg gatgcgcgga cgggacgcaa ggtctgggag
     1081 atccgcgagc gctatccggt gtggagcggc agcgtggcga cggcgggtga cgtggtgttc
     1141 tacggcacgc tggatggatg gttcaaggcg gcggacgcgc gcaccgggaa tcccctctgg
     1201 aagttcaaag tcgggtccgg cgtcgtcggg tgtccgatca cctaccgcgg acccgacggg
     1261 aggcagtacg tcgcggtgta cgcggggatc gggggcgact ggtttctcct gtccggcgac
     1321 gtccgctccg acgatcccgc ggacgtgcgt gcgccggcgg acttcatgcc ggatatcgcc
     1381 cgccacacga gccagggcgg catcgtatgg atcttcgcgc tgtagcggga ctcggtctcc
     1441 tggccgcggc cggatgtcgc ggcgcctcgc gggaggccgg acaggctccg ccctccatcc
     1501 gatacgagca gcacgtggcc gccggcgggg tcgccccggt tgccgcgacc ctggcgaatc
     1561 cgttcgccgg tgatcgcgcg tcggtggcgg agggcgagcg gacgttcgtg gcgatgaact
     1621 gcgacggatg tcacggcggg ggtgcaaccg gctgggtggg cccgagtctc gtggatgggc
     1681 gctggcggta cggcggggcc gacggcgccg tgttccactc catctattac ggccgcgccc
     1741 gcggcatgcc ggcatacggt ggggtgctgt ctcccgacgc gatctggaag atcgtcacct
     1801 acctgcgcgc ccagccgctc cccgccgacg tgcccacgga gcgctggcaa tgacgacgtc
     1861 gggacgatac ggatttctga ccgagaccta cgcgaccgag cgactcaagg tgctgagcgt
     1921 atggagcatg ttcgaggacg gcgacctcgc cgtgcgtccg caccacaccg atgcgcgcgg
     1981 gcggagcgtg ctcgagcaca tggtgcacca gagtctgagc gaggacctgt ggttccggac
     2041 catgctgggc atcgacgtcg gggcgccgcc gttgcccgac acggagacac ggacggcgtt
     2101 cctgcggagt tacgggaagc ggtcggcgca gcgactcacc gcgctgcgcg cgaagcccga
     2161 cgagtggtgg gaggaggaag tcgccttctt cgatgtgcgc cgcagccgtg cctgggtgat
     2221 ggtgcggcgc atcgcccaca ccgcgcatca tcgcgggcaa cagacggcct tgctgcgcat
     2281 gctgggtcgc tcgctgcaca gcacgtacgg tcccactgcc gacacgggag gcctgttcca
     2341 gcacggcgcc gctaccgtgt acgcctacgg agacgaggac gcgctgctca aaggggaagc
     2401 gacgggcggc cgcaaggcgg cgctcccggg acccggcgcg gccccggtca cggagcggcc
     2461 cgcgccatga tgaatgcgga cgcggcgaac ctccccgccc tgatgtgtcg ccgatcctgg
     2521 gaagcggacg acctggtgcg tcgctggccg ccgaagttcg ctcacccgaa caagaaggtc
     2581 gtgtacgacg tgtcctgccc gtcggggacc agccacgagg ggaccgtcac gttctcgcgt
     2641 tggacgggag ggcggctggc gaccgcggtc ccaggggagc gcacagcaga ggtgctggga
     2701 cgacccggct tcttcgacta cgcccgaccg gcggc
//
LOCUS       DBNGZW010000219         3367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_771483, whole genome shotgun sequence.
ACCESSION   DBNGZW010000219 DBNGZW010000000
VERSION     DBNGZW010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_771483"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>3367
                     /gene="rpoC"
                     /locus_tag="AC16JC_05255"
     CDS             <1..>3367
                     /gene="rpoC"
                     /locus_tag="AC16JC_05255"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HIR5164139.1"
                     /translation="KADIGAPAVRELLNKLDVDKVADELRKAVATETSQHRKEQMLKR
                     LKIVDAFRNSGNAGEARNNPQWMILDVVPVIPPDLRPLVPLDGGRFATSDLNDLYRRV
                     INRNNRLQKLILHRAPEVILRNEKRMLQEAVDALFDNGRRSKAIRGRGKRPLKSLSDM
                     LKGKQGRFRQNLLGKRVDYSGRSVIVVGPELRLHQCGLPKAMAVELFKPFIIHKLVEK
                     GIAETVKRAKKIVEKESPEVYEILEEIIEDHPVLLNRAPTLHRLGIQAFEPVLVEGKA
                     IRIHPLVCAAFNADFDGDQMAVHVPLSFESQLEARLLMISSNNVLKPSDGRPVAEPSQ
                     DMVLGCYFLTKEPAEFEARLAKAPRVGSMAELEMGLAVKRLAFHSPVQYWYVPPVGAG
                     PAGWVATTAGRVIFNSILPDMLRKDLGFQNKAMRKRDLSELVFESYRRAGLAPTVQFL
                     DGLKEFGFRYATMGGVSIGVADLEIPSEKASLLHDAEARVERFQRAYSTGQITFGERY
                     NKVIDAWTHANNDIAEAMVATMRKSKGGFNPVFMMFDSGSRGSRDQIRQLAGMRGLMA
                     KPQKKLTGGIGEIIESPIKSNFREGLSVLEYFISTHGARKGLADTALKTADAGYLTRR
                     LVDVAQDVTVTEEDCGTILGLEMAALKEGEDIIEPLRERLVGNVALEEVTDPHELDEH
                     GHPKVLVEAGKPIDEDTAQQIEDAGIETVKIRSVLTCEARRGICRMCYGRNLATMGLV
                     DLGEAVGILAAQSIGEPGTQLTLRTFHIGGTAARIAEQTVRKTKVEGVIEYGERLVFV
                     ETPEGQKVVTSYEGELVLKAAAHGPKDGATGKLAVHSRFHVPLGATLMVEDGQKVARD
                     GVLFTWDPYTNPILTDVPGIVRFVDIMEDETVREELDEITGRRQRVIIEDREKKLHPH
                     IEVVQKKGDKEKRLRDFVIPEGAQLTVEDGQEVHAGLVLAKVSREAYKTRDITGGLPR
                     VAELFEARRPKDPATISEVDGTVRFGDIKRGKREIFVHPEEGEAQVYEVPAGKHLRVH
                     EGDRVRAGDRLTEGPVNPHDILRIKGPRAVQEYLLNEVQEVYRLQGVKINDKHIGVIV
                     RQMLQKVKVVDPGDTEFLEGENVDKLTFRESNQR"
BASE COUNT          625 a         1042 c         1241 g          459 t
ORIGIN      
        1 caaggcggac atcggcgcgc cggcggtgcg cgagctcttg aacaagctgg acgtggacaa
       61 ggtggcggac gaattgcgca aagcggtggc caccgagacc agccagcacc gcaaggagca
      121 gatgctcaag cggctcaaga tcgtggatgc gttccgcaac tccgggaacg ccggcgaggc
      181 acgcaacaac ccgcagtgga tgatcctgga cgtcgtgccg gtgattccgc ccgacctgcg
      241 gccgctggtg ccgctggacg gcgggcgctt cgccacgtcg gacctgaacg acctgtaccg
      301 ccgggtgatc aaccggaaca accggctgca gaagctgatc ctgcaccggg cgccggaagt
      361 catcctgcgc aacgagaagc gcatgctgca ggaggcggtg gacgcgctgt tcgacaacgg
      421 ccgccgctcc aaggcgattc gcgggcgcgg caagcggccg ctgaagtcgc tgtcggacat
      481 gctgaagggc aagcaggggc ggttccgcca gaacctgctg ggtaagcgcg tggactactc
      541 gggccgctcg gtgatcgtgg tgggcccgga gctgcggctg caccagtgcg ggctgcccaa
      601 ggcgatggcg gtggagctgt tcaagccgtt catcatccac aagctggtgg agaagggcat
      661 cgccgagacg gtgaagcggg cgaagaagat cgtggagaag gagtcgccgg aagtctacga
      721 gatcctcgag gagatcatcg aggaccatcc cgtgctcctg aaccgcgcgc cgacgctgca
      781 ccgcctgggc atccaggcgt tcgagccggt gctggtggag ggcaaggcga tccggatcca
      841 tccgctcgtc tgcgccgcgt tcaacgccga cttcgacggc gaccagatgg ccgtgcacgt
      901 gccgctctcg ttcgagagcc agctcgaggc gcggctgctg atgatctcgt cgaacaacgt
      961 gctcaagccg tccgacgggc ggccggtggc ggagcccagc caggatatgg tgctggggtg
     1021 ctacttcctc accaaggagc cggcggagtt cgaggcgcgg ctcgccaagg cgccgcgcgt
     1081 cggctcgatg gcggagctcg agatgggact cgccgtgaag cgcctggcgt tccacagccc
     1141 ggtgcagtac tggtacgtac ccccggtggg ggcagggccg gccggctggg tggcgaccac
     1201 ggcggggcgg gtgatcttca acagcatcct gcccgacatg ctgcgcaagg acctgggctt
     1261 ccagaacaag gcgatgcgca agcgcgacct ctcggagctg gtgttcgaga gctaccgtcg
     1321 cgcggggctc gcccccacgg tgcagttcct ggacggactg aaggagttcg ggttccgcta
     1381 cgccacgatg ggcggcgtgt cgatcggcgt cgcggacctc gagatcccga gcgagaaggc
     1441 gtcgctgttg cacgacgcgg aagcccgcgt cgagcggttc cagcgcgcct attcgacggg
     1501 ccagatcacc ttcggcgagc gctacaacaa ggtgatcgat gcctggacgc acgcgaataa
     1561 cgacatcgcc gaggcgatgg tcgcgacgat gcggaagtcg aagggcgggt tcaacccggt
     1621 cttcatgatg ttcgactcgg gcagccgtgg cagccgcgac caaatccgtc agctggccgg
     1681 gatgcgcggg ctgatggcca agccgcagaa gaagctcacc ggcggcatcg gcgaaatcat
     1741 cgagagcccg atcaagtcga acttccgcga agggctcagc gtgctcgagt acttcatctc
     1801 gacgcacggc gcgcgcaagg gtctggccga cacggcgctg aagacggccg acgccgggta
     1861 cctcacgcgg cgcctcgtgg acgtggcgca ggacgtcacg gtcaccgagg aggactgcgg
     1921 tacgatcctg ggcctcgaga tggccgcgct gaaggagggc gaggacatca tcgagccgct
     1981 gcgcgagcgg ctggtgggga acgtggcgct ggaagaggtc accgacccgc acgagctgga
     2041 cgagcacggc catcccaagg tgctggtgga ggccggcaag ccgatcgacg aggatacggc
     2101 gcagcagatc gaggacgccg gcatcgagac ggtgaagatc cgctcggtgc tgacgtgcga
     2161 ggcgcggcgc ggcatctgcc gcatgtgcta cggccgcaac ctcgcgacca tggggctcgt
     2221 ggacctgggc gaggcggtgg gcattctcgc cgcgcagtcc atcggcgagc cgggcacgca
     2281 gctcacgctg cggaccttcc acatcggcgg cacggcggcg cgcatcgccg agcagacggt
     2341 gcgcaagacc aaggtcgagg gcgtgatcga gtacggcgag cggctggtgt tcgtcgagac
     2401 gcccgaaggc cagaaggtcg tcaccagcta cgaaggcgag ctggtgctga aggccgccgc
     2461 gcacgggccg aaggacgggg ccacgggcaa gctcgccgtg cactcccggt tccacgtgcc
     2521 gctcggcgcc acgctgatgg tcgaagacgg ccagaaggtg gcccgcgacg gcgtgctgtt
     2581 cacctgggat ccgtacacga acccgatcct caccgacgtg ccgggcatcg tgcgcttcgt
     2641 ggacatcatg gaggacgaaa cggtccgcga ggagctggac gagatcaccg gccggcgcca
     2701 gcgggtcatc atcgaggacc gcgagaagaa gctccatccg cacatcgagg tcgtccagaa
     2761 gaagggcgac aaggagaagc ggctgcgcga cttcgtcatc cccgagggcg cccagctcac
     2821 ggtggaggac ggccaggagg tgcacgccgg tctggtgctg gccaaggtga gccgcgaggc
     2881 gtacaagacg cgcgacatca ccggcggcct gccgcgggtg gccgagctgt tcgaggcgcg
     2941 gcgtcccaag gacccggcca ccatttccga ggtggacggc acggtgcgct tcggcgacat
     3001 caagcgcggc aagcgcgaga tcttcgtcca ccccgaggag ggggaggcgc aggtctacga
     3061 ggtgccggcc gggaagcact tgcgggtgca cgagggcgac cgcgtgcgcg ccggcgaccg
     3121 gctcaccgag ggtccggtga acccgcacga catcctgcgg attaagggcc cgcgcgcggt
     3181 gcaggagtac ctgctgaacg aagtgcagga ggtgtaccgc ctgcagggcg tgaagatcaa
     3241 cgacaagcac atcggcgtga tcgtgcgcca gatgctgcag aaggtgaaag tcgtggatcc
     3301 gggcgacacc gagttcctgg agggcgagaa cgtggacaag ctcacgttcc gcgagtcgaa
     3361 ccagcgg
//
LOCUS       DBNGZW010000220         4089 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_632057, whole genome shotgun sequence.
ACCESSION   DBNGZW010000220 DBNGZW010000000
VERSION     DBNGZW010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4089)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_632057"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(269..763)
                     /locus_tag="AC16JC_05260"
     CDS             complement(269..763)
                     /locus_tag="AC16JC_05260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164140.1"
                     /translation="MPRSPSTRHPRATRLHASWNPPSGQDPGATITLTYAGQEYRREV
                     GPEDLVTQREAALLLGVSVMAVNKHVRARALQASVHGGVSVIRLSEVCRFARDRGLSP
                     EAVRPLPWLGQLVRQLAPPLARLDEASVWQPLTLQERVRAPRSAQRSRSAARPRRKKR
                     RATD"
     gene            complement(960..1042)
                     /locus_tag="AC16JC_05265"
     tRNA            complement(960..1042)
                     /locus_tag="AC16JC_05265"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1007..1009),aa:Leu,seq:tag)
     gene            complement(1667..3757)
                     /locus_tag="AC16JC_05270"
     CDS             complement(1667..3757)
                     /locus_tag="AC16JC_05270"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5164141.1"
                     /translation="MRVALVWSVIAGFAAPLAAQVPLGFRVADTAAQRALEARLLAVP
                     DTATPRRLTADLSARPHMSGTPAQAATRDYVIDRLRAAGLETWVKAYDVYLPHVDTVQ
                     AWLLPRRGAAPQRLSLAEPAVAGNPVTAGPQVPPFNGYTGDGDVTAEIVFVNYGLIED
                     YKTLDSLGVSVRGKIAIARYGRSFRGIKAREAERHGAVGLIMYSDPQDDGYVRGDVYP
                     KGPMRPAGGIQRGSILNMDGDPTTPGYASVPGARRVPEDSLRVPHIPVMPMGYGNAQR
                     LLAALGGPSVPGGWQGGLPFRYHVGPGQTMAHLKVRREHGSRAMHTIWDTFGMIRGAE
                     YPDEWIVVGGHRDSWSPGAADDISGTVTVLEAARAFAELARRGIRPARTLVFATWDAE
                     EWGLIGSTEFVEEMADSLRAHVVAYINEDDMTEGPSFGGGGSPSLKRLMREVTRAVPD
                     PVGPGSVYDVWLKRVKGDTTALSLGNVGGGSDFAGFYHHLGIPAAGVGFGGPSGVYHS
                     MYDSFDWMNRFGDPGWREHQAAARLVAVLLARLGNARVVPLDYVGWGGEMTQLVGQLD
                     SAIARRGWSVSTQGLKDALGRFTATALSFAAARDSALAVGADRPWARVNAALMQVERR
                     FTRPEGLASDRWFKSLQFASDVDNGYATLAFPTVSEAARYADAAMAERELADAVTRLE
                     ASRAALEEAATALR"
     gene            complement(3904..>4089)
                     /locus_tag="AC16JC_05275"
     CDS             complement(3904..>4089)
                     /locus_tag="AC16JC_05275"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5164142.1"
                     /translation="DPAALRLAIAFAGAGQILAGSDYPHRIGSIPAMLESLRGLDVPE
                     QDRAAMLGGNAAQLLGI"
BASE COUNT          606 a         1490 c         1383 g          610 t
ORIGIN      
        1 gcgcgggcat accatacgag ccccagcgaa agcagcacca gcgacgccgc ggcggtgagc
       61 ggccagtacg gtggcaggcg cgtgggccac ccgctcacgt ggtggctcgg cggctggggg
      121 tcagggcggt acggggcgcc atcatagggc tccggcgctt caggctccgt gccaccggga
      181 cgtcaggaag gccttgattc ccgtggggcg caaggcgctt cgccgtcttg aacgcaccaa
      241 ttgggtgcgg gagctgcggg gaaccgccct aatctgtcgc gcgccgcttc ttccggcgcg
      301 gacgtgcggc gctcctgctc cgctgcgcgg accgtggcgc gcgcacccgt tcctgcaacg
      361 tcagtggctg ccacacgctc gcttcgtcca ggcgggcgag cggcggcgcg agttgacgca
      421 ccagctgtcc cagccagggc agtggtcgca cggcctcggg gctcagcccg cggtcgcgcg
      481 cgaagcggca tacctcgctc agccgaatga ccgacacgcc accgtgcacc gacgcctgca
      541 acgcgcgcgc gcgcacgtgc ttgttgacgg ccatcacgct caccccgagg agcagcgcgg
      601 cctcccgttg ggtgaccaag tcctcgggtc cgacctcgcg ccggtactcc tgccccgcgt
      661 aggtgagcgt gatcgtggcg cccggatctt gtccggaggg tgggttccag ctggcgtgca
      721 accgggtcgc gcgcggatgt cgggtggagg gggaacgtgg catgagcggg ccgatatcac
      781 gtggtaaacg tcattggcga cgcgtcgggg tgccatggcg acgccaggtg ctccatttat
      841 cacctggtaa acacccgtcc gaccgttcga ggctgcgtac cacatcagcg gtgccgcggg
      901 cgtatcgatg aagtgcctgc tacgacagga gcgcccgccg gatcatcggt catcgcaggt
      961 gctcgggggg agactcgaac tcccacgccc ttgcgggcac tggctcctaa ggccagcgcg
     1021 tctgccgttc cgccacccga gcgctagggc ttctggattc gcctgcgcag cgtcgacacc
     1081 gcgagccgaa aggcgcgtac gtcattaggc ttgagccgct tcatcttctt caggaacgca
     1141 tcgaggtgcc ggatgtcgcg cacccgtttt gcacgctcgg ctctccacaa cgtctcgaac
     1201 ctgcggacct aacgccgcca ctcggctctc agcttctcag ctgcgtgttt gccagccatg
     1261 agcccccgtc ctgtatcact cacactaatc tatagaaacg gggagggtcc gcgatgcccc
     1321 gtcgagcact tcacctggga tccccgggca ggctccggca ccagcctgtt cggggaggtg
     1381 gatgtgtctt aaggcggcgt caccagcgtc tcttgtagtc gttcagggga tgggggacag
     1441 catgcgaggt ggcagttctg gccggcgcca tgtcgacatc gttccgggac ggcggtcacc
     1501 caggccgata ctactcgccc accgatgtca accgagctca acctgccgca agactcagta
     1561 ctgacggtcg cgccaccggc cgggcaacgg gaagaacgat cgcagtcgct cggcgatggc
     1621 atccttcccg acgcggccat ccgcaaccgc catcgtcaga tcgaggctac cggagcgcgg
     1681 tggccgcttc ctccagcgcc gcccgcgacg cctcgagccg ggtcaccgca tcggccagct
     1741 cccgctccgc catcgctgcg tcggcgtacc gcgccgcctc gctcacggtg gggaaggcga
     1801 gcgtcgcgta gccgttgtcc acatccgacg cgaactggag gctcttgaac caccggtcgc
     1861 tcgccagccc ctcgggacgc gtgaaccgtc gctccacctg catgagcgcc gcgttcacac
     1921 gtgcccacgg acggtcggcg ccgacggcga gggcgctgtc ccgcgcggct gcgaagctca
     1981 gggctgtcgc cgtgaaccgt cccagcgcgt ccttgagccc ctgcgtgctg acgctccacc
     2041 cgcgccgggc gatcgcgctg tcgagctgcc ccaccagctg cgtcatctca ccgccccagc
     2101 cgacgtaatc gagcggcacg acccgcgcgt tgccgagccg cgccagcagc accgcgacca
     2161 ggcgtgccgc cgcctggtgc tcgcgccacc caggatcgcc gaaccggttc atccagtcga
     2221 acgagtcgta catcgagtgg tacacgccgg acggcccgcc gaacccgacg ccggccgccg
     2281 ggatccccag gtggtgatag aacccggcga aatcggagcc gccgccgacg ttgccgaggc
     2341 tgagcgcggt ggtatcgccc ttcacgcgtt tcagccagac gtcgtacacg gagcccggcc
     2401 ccacgggatc cggcacggcc cgcgtcacct cgcgcatcag ccgcttgagc gagggcgagc
     2461 cgccgccgcc gaagctggga ccctccgtca tgtcgtcttc gttgatgtag gccaccacgt
     2521 gggcgcgcag gctgtccgcc atctcctcca cgaactccgt ggagccgatc aggccccatt
     2581 cctcggcgtc ccaggtggcg aacacgaggg tgcgggcggg ccggatgccc cggcgcgcca
     2641 gctccgcgaa ggcgcgggcc gcctcgagca ccgtcacggt gccgctgatg tcgtccgcgg
     2701 cccccgggct ccacgaatcg cgatgccccc ccacgacgat ccactcgtcc gggtactccg
     2761 cgccgcggat catgccgaac gtgtcccaga tcgtgtgcat cgcccggctg ccgtgctcac
     2821 gccggacctt caggtgcgcc atcgtctgcc ccggccccac gtgataacgg aagggcagac
     2881 cgccctgcca gccccccggc acgctcggcc ctcccagggc ggcgagcaac cgctgcgcat
     2941 tgccgtagcc catcggcatc acgggaatgt gcggcactcg cagggagtct tccggcaccc
     3001 ggcgcgcccc gggcaccgac gcgtaccccg gcgtcgtggg atcgccgtcc atattgagga
     3061 tgctgccgcg ctgaatcccg ccggccggcc gcatcggtcc cttggggtac acgtcaccgc
     3121 gcacgtaccc gtcgtcctgg ggatcggagt acatgatcag gccgacggcg ccatggcgct
     3181 cggcttcccg cgccttgatg ccgcggaacg agcggccgta gcgcgcgatg gcgatcttgc
     3241 cgcgcacgct cacgccgagc gaatcgaggg tcttgtagtc ctcgatcaac ccgtagttga
     3301 cgaatacgat ctcggccgtg acgtcgccgt cgccggtgta cccgttgaac ggcgggacct
     3361 ggggcccggc ggtgacggga ttgcccgcga ccgccggctc ggcgagggac agacgctggg
     3421 gtgccgcccc gcggcggggc agcagccacg cctggaccgt atcgacgtgg ggtaggtaca
     3481 cgtcgtacgc cttgacccag gtctcgagcc ccgcagcccg caggcggtcg atcacgtagt
     3541 cgcgcgtcgc ggcctgggcc ggggtccccg acatgtgcgg ccgggcggac aggtcggcgg
     3601 tgaggcgccg tggcgtcgcc gtgtcgggca cggcgagcag ccgggcttcg agcgcccgtt
     3661 gcgcggccgt gtcggcgacg cggaagccga gcggcacctg tgccgcgagc ggagcggcga
     3721 aaccggcgat cacggaccag accagtgcga ctctcacgag tcctccgaag gtgtgagaac
     3781 ggcgtgcgga gacgagacga gccccaacat aaccccgggg ctcccccgcg tcccctcgca
     3841 agggtgcatg ggtgcaccgc actcggtggc acggccgtcc gccgtcccca tcagcctccg
     3901 gcactagatc cccaggagtt gcgcggcgtt gcccccgagc atcgccgccc ggtcctgctc
     3961 cggcacgtcc agtccgcgca ggctctcgag catcgcggga atacttccga tgcgatgcgg
     4021 atagtcgctc ccggcgagga tctggcccgc gcccgcgaac gcgatggcca gccgcagcgc
     4081 ggccggatc
//
LOCUS       DBNGZW010000221         1402 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1655027, whole genome shotgun sequence.
ACCESSION   DBNGZW010000221 DBNGZW010000000
VERSION     DBNGZW010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1402)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1402)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1402
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1655027"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            480..>1402
                     /locus_tag="AC16JC_05280"
     CDS             480..>1402
                     /locus_tag="AC16JC_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-3-methylglutaryl CoA synthase"
                     /protein_id="HIR5164143.1"
                     /translation="MGDEVGILSFGAYIPRLRLQRKAIAAANSWFNPGLRGLAKGERA
                     MASWDEDAVTMAVEAARDCLAGFDHHAVASLYLASTTHPFDDRQNAGIAAGALHLRPA
                     LRSLDAGGSLRVATSTLATALAAAAGTEGPTLLAAGDHRLAKAASPQEMQYGDGAAAL
                     LIGWGRPIAQLVGAHSETVDFVPQFRAHDRAHDYAWEERWIRDEGYMKIVPRTIGALL
                     KATGTEASAVSAFCLPCTLSRVVPGVGKRVGIADGAVRDNLAAVCGDTGAAHPLVMLV
                     HALEGAKPGDLVVVAAFDSGCDALLFRVTDAV"
BASE COUNT          217 a          490 c          474 g          221 t
ORIGIN      
        1 cggagatggt gccgtcctcg ctggcgaaga tgaaacggtc cgggctcgtg ccctgcccat
       61 tgttcacgac gaagccggga cccccgttga acacgacgcc ggtcggtgtg ccctgctgac
      121 cgcccgcgcc aggaaccgtg aacgtattcg tgatggtgcc ggtcccgacg gaaaggggtc
      181 aaggcgatcc agcagcgctc cagcaggcag cctcggggtg aatcgctgtc aacttattag
      241 atagtctctg cgattcaccc gctaggcgaa tcgtggtcaa ctccggccgt gccacgcttc
      301 gggtaccacg ggaagctgag cacgtacccg aaggccaccg accctttgaa ccacggtaga
      361 gcgggaccgg agtcgtcccc cacgaaggcg gcgtcctctg ctaatctaac ggcgtgctgg
      421 gtggtggtga cagtcggcgt ctcgctcggg acgtagctca acccattcga ggaaaggtta
      481 tgggagacga agtcggaatc ctctccttcg gtgcctacat cccgcggctc cggttgcagc
      541 gcaaggccat cgccgcggcc aactcctggt tcaaccccgg gctccggggt ctggccaaag
      601 gcgagcgcgc gatggccagc tgggacgagg atgcggtgac catggcggtc gaggcggccc
      661 gcgactgcct cgccggcttc gaccaccacg cggtcgcctc gctgtatctc gcctccacca
      721 cccacccctt cgacgaccgg cagaatgcgg gcattgccgc tggcgcgctg cacctgcgtc
      781 cggccctgcg ctcgctcgat gccggcggca gcctgcgcgt cgcgacctcg acgctcgcca
      841 ccgccctcgc ggcggcggcc gggacagagg gcccgaccct cctcgccgcg ggcgaccacc
      901 ggcttgccaa ggcggccagc ccgcaggaga tgcagtacgg cgacggcgct gcggccctgc
      961 tgatcgggtg ggggcggccg atcgcccaac tcgtgggcgc gcacagcgag acggtcgact
     1021 tcgtgccaca gttccgtgcg cacgaccgcg cccacgacta tgcgtgggag gagcgctgga
     1081 tccgcgacga gggatacatg aagatcgttc cgcggaccat cggcgccctg ctcaaggcaa
     1141 ccggcacgga ggcatccgcg gtgagcgcct tctgcctgcc gtgcacgctc tcacgcgtcg
     1201 tcccgggggt cgggaagcgc gtgggcatcg cggacggcgc cgtgcgtgat aacctcgctg
     1261 ccgtgtgcgg cgataccggc gccgcacatc cactcgtgat gctcgtccac gcgctcgagg
     1321 gagccaagcc cggcgatctc gtcgtcgtgg ccgccttcga cagtggctgc gacgcgcttc
     1381 tgttccgtgt caccgacgcg gt
//
LOCUS       DBNGZW010000222         5972 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_423223, whole genome shotgun sequence.
ACCESSION   DBNGZW010000222 DBNGZW010000000
VERSION     DBNGZW010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5972)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5972)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5972
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_423223"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2810)
                     /locus_tag="AC16JC_05285"
     CDS             complement(<1..2810)
                     /locus_tag="AC16JC_05285"
                     /inference="COORDINATES: protein motif:HMM:NF017964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HIR5164144.1"
                     /translation="MRRVALLAVGAGADAAWELLGALPDVTPRRLAPDGWSEGLAQAD
                     LLWVHTEEEPPPMPREVVVPWVERGGRLLLTQRAAACVVALGLEADGPNDTAARVWRH
                     EDDELWFPDMRSFGAFPHVRGLAAFGSHPLFDGLAQGAFVWAPSEGERYVQTTYARGR
                     RPARGRVVACERSYIHVNADRVVGWEYEVGAGGVLCIGAFVVPGARDALLARQLRIVL
                     ANALAAQGIPHRSREAPAAAWPEPGRRAHEDPNLVLPDRVRLDGPLPGLDSPLHVASR
                     ALSDEAVTLAGRRVLIVGGEQRGVREIWAHPYRLLRDLTVTVGGEAPLIRDAQITPLL
                     IQRHLVSRARIVEETVTSALDHPIVLIEYRPEKVGRARGLRAPTELLLKWQVDLRRTW
                     PYPAGVGGDLRYRLAPHGATLVVTDGTGLPRAAFVVSGGACEWRLHPVEEQPVLSCEL
                     RTALEAPFRFAVLGGASRAELDGAVAALGRRGVAGLSAQRSRHEGQVQEHLVQLRSPE
                     PGLNSAFEWAKSRLDGCFVETPGVGRSLIAGYAPSRLGWGDGRPGYAWYFGRDACWTA
                     FALLAAGDYAGCRLVLRFLGSTQDVSGKVIHEYTTSGLAHYDAADATPLYLLLAGRYA
                     AWTADLAFLAERWPEIERAYRFCLETDTDGDGLIENTRVGHGWIEMGPLSGAHVTLYL
                     ASVWVAALAALEPVALALGHRALGQELAERAARARARIAKGFRLADGYALGLLADGTQ
                     QRSRTALTAVALLLDAVDPAPATAWLDAIAAPGFSTPWGVRLIPREDPHYSPAGYHSG
                     AVWPLYTGWVSLAEFACHRAEQGFTHLLANARLPFAHGQGSFAEVLDGDLGTPAGVCP
                     DQAWSAAMLISPVIEGMLGARPDALASRLTLAPHLPSGWRACEWRGLHVGHTTLDVRV
                     GTLPDRIQVRVRRTAGGHLAVTV"
     gene            complement(2812..4995)
                     /locus_tag="AC16JC_05290"
     CDS             complement(2812..4995)
                     /locus_tag="AC16JC_05290"
                     /inference="COORDINATES: protein motif:HMM:NF046269.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGH1-like glycoside hydrolase domain-containing
                     protein"
                     /protein_id="HIR5164145.1"
                     /translation="MTDALRLLARPDKWYLGAGDGLIWAPPFPRWLDTPGFWDEAHLF
                     QYAIAPGFTVSFVVDGAPLPARCRRRHWSPATLTLDHALGSLRARETRSAPGGALTTR
                     WDVRNPGARPVTLDVVAWTAVDGEELAAGGVTVRRGALSFVRGVVDRQKHRAAVRSVL
                     ALTPSAQSFAGYRSQGTAAALPPRFAVTPFWDRYQPSGRLRRELHLGGIDPRGVVFLG
                     VQRRFRIAGRGRVSFEGALALALEGGLPTSRRSTGDWRRYFAGVPQFHCSDPHLDHQW
                     WYRWYGLRLHGIAGGSAPSYRHPTVCEGIHYFHTPISYSAQCHLRELRWLPDPAWGRG
                     VLRTFLDHQRPDGSLPGRIPADHVEGPDFYHADWGGAMLALDAVHPDASFRREAYAGL
                     VRYADWLRRTRDRDASGMVDVVDQQETGQEYTSRYVAVDPVADRDEWANRIRLKGIDA
                     TVYGYRLFRALAHLAAEIEPAAADGWHTAADRTRQAVRGAMWDPELEMFSDVDPRLGH
                     RTGVKAAVCFYPYGTDLADHQHLAGLRRHLFNRREFWTAFPVPSASVDDPNFNPDAEW
                     KGKRHLCPWNGRVWPITNSHLVDALAHVVRAHRPEWAPRLGALLRRFARMMSDDGRPD
                     RPNAFEHYHPGSGRPSRYRGIDDYQHSWLIDLIVSNVLGVLPHGATGLTVHPLALGIA
                     AARVAGLSVAGHRVDITIAGPGYRVRVDGLRAGAGRVGEPLTVDF"
     gene            complement(4992..5465)
                     /locus_tag="AC16JC_05295"
     CDS             complement(4992..5465)
                     /locus_tag="AC16JC_05295"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR5164146.1"
                     /translation="MSEATSSPPRVGDPAPDFTLPSTAGADVTLSAFRGRQNVLLAFF
                     PLAFTSTCTEENCSFSEHLDAFDGTDTVVLPISVDSVATLKEYKAKYAMRQDLLSDFK
                     RDVSRRYGTLLDDKFFSSRAYFVIDRQGVLRWQHTEAKLGDRRDDAELLRVLRSL"
     gene            5609..>5972
                     /locus_tag="AC16JC_05300"
     CDS             5609..>5972
                     /locus_tag="AC16JC_05300"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HIR5164147.1"
                     /translation="MPHTLPPLPYDFAALEPHIDTQTMQIHHGKHHQAYVNNLNAALD
                     KHPEWHQKSLDELLRGINSVPEDIRTAVRNNGGGHHNHSLFWTSMAPRAGGEPSGKLA
                     DAIGTALGGFAKFKDSFTA"
BASE COUNT          952 a         2192 c         2077 g          751 t
ORIGIN      
        1 acggtcaccg cgagatgacc accggcggtc cgccgcacgc gcacctggat gcggtccggc
       61 agggtgccga cccggacgtc cagcgtcgtg tgccccacgt gcagaccgcg ccattcacac
      121 gcacgccacc ccgagggcag gtgcggcgcc agcgtcaggc ggctcgcgag ggcgtccggc
      181 ctcgcgccga gcattccctc gatcaccgga gagatgagca tcgccgcgga ccacgcctga
      241 tcgggacaga cgccggcggg cgtcccgaga tcgccgtcca gcacttcggc aaacgatccc
      301 tgtccgtgcg caaacggcag ccgggcgttc gccagcaggt gggtgaaccc ctgctccgcg
      361 cgatggcagg cgaactcggc gaggctgacc caccccgtgt agagcggcca gaccgcgccg
      421 ctgtggtagc cggcgggact gtagtggggg tcctcacgcg gaatcaggcg cacgccccag
      481 ggcgtggaga accccggcgc ggcgatggcg tcaagccacg cggtcgccgg tgcggggtcc
      541 accgcatcga gcagcagggc cacggccgtc agcgccgtgc gcgaccgctg ttgcgtgccg
      601 tcggccagca gacccagggc gtacccgtcg gccagccgga accctttcgc gatgcgggcg
      661 cgggcccgcg ccgcgcgctc cgccagctcc tggcccaggg cgcggtggcc gagtgccagc
      721 gcgacgggct cgagcgcggc gagcgcggcc acccagacgc tggcgaggta gagcgtcacg
      781 tgggcgcccg agagcggccc catctcgatc cacccgtgcc ccacccgcgt attctcgatc
      841 aggccgtcgc cgtcggtgtc ggtctcgaga cagaaccgat aggcgcgctc gatctcgggc
      901 caccgctcgg ccagaaatgc gaggtcggcg gtccatgccg catagcggcc tgccagcagc
      961 agatacagcg gcgtcgcatc ggccgcgtcg tagtgggcga gtccgctggt ggtgtattcg
     1021 tggatcactt tgccgctcac gtcctgggtg gagcccagaa accgcaacac gagccggcac
     1081 cccgcgtagt cccccgcagc gagcagggcg aacgcggtcc agcatgcgtc acgtccgaag
     1141 taccacgcat agccgggacg cccgtccccc caaccgaggc gggacggcgc gtacccggcg
     1201 atgagcgagc gtcccacgcc cggcgtctcc acgaagcagc cgtcgaggcg gctcttggcc
     1261 cattcgaacg cgctgttgag tcccggctcg ggcgagcgta gctggacgag atgctcctgc
     1321 acctggccct cgtgccgcga ccgctgcgcc gagagcccgg cgacgccgcg acgacccaat
     1381 gcggccaccg cgccatcgag ctcggcgcgg gacgcgccgc ccagcaccgc gaaccggaac
     1441 ggcgcctcga gcgccgtccg cagctcgcac gaaaggaccg gctgctcctc gaccggatgg
     1501 aggcgccatt cgcacgctcc gccgctcacc acgaacgccg cccgcggcaa tcccgtcccg
     1561 tcggtgacga ccagcgtggc gccgtgcggc gccaggcgat agcgcagatc cccgcccacg
     1621 cccgcggggt acggccaggt gcgccgcaag tccacctgcc acttgagcag cagctcggtc
     1681 ggcgcgcgca gtccgcgggc gcgccccacc ttctccggcc ggtactcgat cagcacgatc
     1741 gggtgatcca gtgcgctggt cacggtctcc tcgacgatgc gcgcccggct cacgagatgc
     1801 cgctggatca ggagcggggt gatctgtgcg tcgcggatga gcggggcttc accgcccacg
     1861 gtgaccgtga gatcgcgcaa caatcggtac gggtgcgccc agatctcccg gacgccgcgc
     1921 tgctcgccgc ccacgatgag gacgcgccgg ccggccagcg tgacggcctc gtcggaaagc
     1981 gcccgactcg cgacgtgcaa cggggaatcc agaccgggca gcgggccgtc gagccggaca
     2041 cggtcgggca gcaccagatt cggatcctcg tgcgcgcggc gcccgggctc cggccacgcc
     2101 gcggcggggg cctcacggct gcgatggggg atcccctgcg cggcgagcgc attcgcgagc
     2161 acgatgcgca gctgtcgcgc cagcagcgcg tcgcgcgcgc ccggcacgac gaacgcaccg
     2221 atgcacagca cgccgcccgc accgacctcg tattcccagc cgaccacccg atccgcgttg
     2281 acgtggatgt aggagcgctc gcacgccacc acgcgcccgc gcgcgggacg ccgaccgcgc
     2341 gcgtacgtcg tctgcacgta ccgctcccct tcgctcggcg cccacacgaa ggcgccttgc
     2401 gcgagcccgt cgaacaacgg gtgactgccg aacgcggcca gcccgcgcac gtgggggaac
     2461 gccccgaacg agcgcatgtc cgggaaccac agctcgtcgt cctcgtgtcg ccagacccgg
     2521 gccgccgtgt cgttggggcc gtcggcttcg agccccaggg cgaccacgca ggccgcggcg
     2581 cgctgcgtga gcagcaggcg gccgccccgc tccacccatg ggacgacgac ctcccggggc
     2641 atcggaggcg gctcctcctc ggtatgcacc cagagcagat cggcctgcgc gagcccctcc
     2701 gaccagccgt ccggcgcgag ccggcgcggc gtgacgtcgg gaagcgcgcc caggagctcc
     2761 caggccgcat ccgccccggc gcccaccgcg agcagcgcga cgcgacgcac gtcagaagtc
     2821 cacggtgagc ggctcgccca cccgtccggc gccggcgcgg agaccgtcca cgcgcacgcg
     2881 gtatcccggt ccggcaatgg tgatgtccac gcggtgaccg gccacggaca ggcccgccac
     2941 ccgcgccgca gcgataccga gcgccagggg atggacggtg agcccggtcg cgccgtgcgg
     3001 cagcacgccg agcacattgg acacgatcag atcgatgagc cacgaatgct gatagtcgtc
     3061 gatcccgcgg taccggctgg ggcggccgct ccccgggtga tagtgctcga acgcgttggg
     3121 ccggtcgggg cggccgtcat cggacatcat gcgcgcgaag cgccgcagca gggcacccag
     3181 gcgcggcgcc cattccggcc ggtgcgcccg gactacgtgc gccagggcat ccacgaggtg
     3241 cgaattggtg atcggccaga cacggccgtt ccacgggcag aggtggcgct tccctttcca
     3301 ctccgcatcg ggattgaagt tcgggtcgtc cacgctcgcc gagggcacgg ggaacgccgt
     3361 ccagaattcc cggcggttga acagatggcg gcgcagtccg gcgagatgct ggtggtccgc
     3421 gagatcggtc ccgtacggat agaagcacac cgcggccttc acgccggtgc gatggcccag
     3481 ccgcggatcc acgtcgctga acatctcgag ctccgggtcc cacatcgcgc cgcgcaccgc
     3541 ctgccgcgtg cggtcggccg ccgtgtgcca cccgtccgcg gctgccggct cgatctccgc
     3601 agcgagatgc gccagcgcgc ggaacaggcg gtaaccgtac accgtcgcgt cgatgccctt
     3661 caaccggatg cggttcgccc actcgtcgcg gtccgccacg ggatcgacgg ccacgtaccg
     3721 cgacgtgtat tcctggccgg tttcctgctg gtccaccacg tccaccatgc cgctcgcgtc
     3781 ccggtcgcgc gtgcggcgca gccagtcggc gtaccggacc agcccggcgt atgcctcacg
     3841 gcgaaacgac gcgtcggggt ggacggcatc gagcgccagc atggcgccgc cccagtcggc
     3901 gtggtagaaa tcgggcccct ccacgtggtc ggccgggatg cggcccggga gcgatccgtc
     3961 ggggcgctgg tgatcgagaa acgtacggag cacgcctcga ccccacgccg ggtccggtag
     4021 ccagcgcaac tcccgcaggt gacactgggc gctgtagctg atgggcgtgt ggaaatagtg
     4081 gatgccctcg cacacggtcg ggtgccggta gctcggcgcc gacccccccg cgatgccgtg
     4141 gaggcggagc ccgtaccagc gataccacca ctgatgatcg agatgggggt cgctgcagtg
     4201 aaactggggc acgcccgcga agtagcgacg ccaatcgccc gtgctgcggc gcgacgtcgg
     4261 cagtcctcct tcgagcgcca gggcgagcgc cccctcgaac gaaacgcggc cccgccctgc
     4321 gatccggaag cggcgctgca cgccgaggaa gacgacgccc cgcggatcga tgccccccag
     4381 atgcagctcg cggcgcaacc ggccggacgg ttggtaccgg tcccagaacg gcgtcaccgc
     4441 gaaccggggc ggcagggccg cggcagtccc ctgcgagcga tagccggcga acgactgtgc
     4501 cgagggcgtc agcgcgagga ccgatcgcac ggcggcacga tgcttctggc gatccacgac
     4561 gccccgcacg aacgacagcg ccccgcgccg caccgtgacg ccgccggccg cgagctcttc
     4621 gccgtccacg gcggtccacg cgacgacgtc gagcgtcacc ggccgcgcgc cggggttgcg
     4681 cacatcccac cgcgtggtca gggcgccgcc cggcgcgctg cgcgtctccc gggcgcgcaa
     4741 tgagccgagc gcatggtcga gtgtgagggt ggccggcgac cagtggcgtc gccggcagcg
     4801 ggccgggagc ggcgcgccgt ccacgacgaa gctcacggtg aatcccggcg cgatcgcata
     4861 ctggaacagg tgcgcctcgt cccagaaccc cggggtgtcg agccagcgcg gaaacggtgg
     4921 cgcccagatc agtccgtccc ccgccccgag gtaccacttg tcaggccggg cgaggagccg
     4981 aagggcgtcg gtcacagcga ccggaggacg cggagcagct cggcgtcgtc gcgccggtcg
     5041 ccgagcttgg cctcggtgtg ctgccagcgc agtacaccct gccggtcgat gacgaaatag
     5101 gcgcgactcg agaagaactt gtcgtccagg agcgtcccat agcggcggct gacgtcgcgc
     5161 ttgaaatcgc tcaacaggtc ctgccgcatg gcgtacttcg ccttgtactc cttgagggtg
     5221 gcgacggaat cgacgctgat cggcagcacc acggtgtcgg ttccgtcgaa cgcgtcgaga
     5281 tgctcactga aggagcagtt ctcctccgtg cacgtgctgg tgaacgcgag cgggaagaag
     5341 gcgagcagca cgttctggcg accgcgaaag gccgacaggg tcacgtccgc cccggcggtg
     5401 gagggcaggg tgaaatcggg agccggatca cccacgcgcg gagggctgga tgtggcttcg
     5461 gacactggtg agctctccca cacgaacggg tcggggtgag ggccgaaatg tcgtcgcggg
     5521 aactattcac cgcaacggcg gcgtttgatt tcccgaacag ccgtgaccat tttcccggtc
     5581 tcctctagat cccggagtag acagcaccat gccccatacc ctgcctcccc tgccgtacga
     5641 tttcgcggcc ctcgagcccc acatcgacac ccagacgatg cagatccacc acggcaagca
     5701 tcaccaggcc tacgtcaaca acctcaacgc cgcgctcgac aagcaccccg agtggcacca
     5761 gaagagcctg gacgagctgt tacgcggcat caacagcgtc cccgaagaca tccggacggc
     5821 ggtgcgcaac aacggcggcg gccatcacaa ccactcgctg ttctggacca gcatggcccc
     5881 cagggccggc ggcgagccga gtggcaagct ggccgacgcg atcggcacgg cgctgggcgg
     5941 cttcgccaag ttcaaggatt cgttcacggc cg
//
LOCUS       DBNGZW010000223         2029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1786372, whole genome shotgun sequence.
ACCESSION   DBNGZW010000223 DBNGZW010000000
VERSION     DBNGZW010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2029
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1786372"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="AC16JC_05305"
     CDS             complement(<1..529)
                     /locus_tag="AC16JC_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164148.1"
                     /translation="MLPAAATGTRGQWWRLVLGTIVLLPFAPGSLDLVAPTLAALLVA
                     SAAGSRRSRELAIAAVLAGLGLAAVIWSSSKADPLARALSTYSLLAAVAFAGGALLAP
                     AGVLRQAFRAVCWAGVGVGLLGLLVRGAGFWTELHWSALRQTSSAFRTIVPLRPDAYP
                     LLEPLVRLMSDAFPAF"
     gene            complement(529..753)
                     /gene="rpsR"
                     /locus_tag="AC16JC_05310"
     CDS             complement(529..753)
                     /gene="rpsR"
                     /locus_tag="AC16JC_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012566762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR5164149.1"
                     /translation="MRRPAKTCPVCESGVRVLDYKDDRTLGRFLTERGKILPSRLSGM
                     CARHQRQLATAIKRARQLALIPYIKSGSPA"
     gene            complement(750..1115)
                     /gene="rpsF"
                     /locus_tag="AC16JC_05315"
     CDS             complement(750..1115)
                     /gene="rpsF"
                     /locus_tag="AC16JC_05315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR5164150.1"
                     /translation="MTRRYETVYIFDSALEEQAINEKLERFHALLTKSGSGTVSGSNH
                     WGKRTLAYPIKKKETGYYVVTQFEATGDLLPEYERAVKLEESVLRYLIVLNEGEPPKP
                     MPTTPPVVAEELELEEEEA"
     gene            1276..1983
                     /locus_tag="AC16JC_05320"
     CDS             1276..1983
                     /locus_tag="AC16JC_05320"
                     /inference="COORDINATES: protein motif:HMM:NF025092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR5164151.1"
                     /translation="MRLTFLAVALWAIPIALGAQQDSARLTGTVTSLSGAPLSGVLLS
                     LPEEGWSHTTDSTGRFEHLTTRPGRRTLRVAVIGIAPQEYDVQLHAGRTTAITVLVDP
                     AAIELEPIVVIGEHEEGSLRIDGFYARKLRAVYGGLYFTHADIMRRAPDRLSTLLGAT
                     GLTMRCGLVGGGCVPVARCIVPVYLDGMRVPSRELDRIPPSMVAGVEVYRSSFDTPLE
                     YAIQGGSCGAILVWTGV"
BASE COUNT          311 a          685 c          720 g          313 t
ORIGIN      
        1 cgaacgccgg aaaggcgtcg ctcatgaggc ggaccagcgg ctcgagcagg gggtaggcgt
       61 ccggccgcag cgggacgatg gtccggaacg cgctcgacgt ctgacgcagc gcgctccagt
      121 gcagctcggt ccagaagccc gcgccccgca cgaggaggcc gagcagtccc acccccacgc
      181 cggcccagca cacggcgcgg aacgcctggc ggagcacccc ggccggcgcc aggagcgcgc
      241 cgcccgcgaa cgccacagcc gcgagcagcg agtacgtgct cagcgccctc gcgagcggat
      301 cggctttgga ggaggaccag atgacggcgg cgagtcccag ccccgcgagc accgcggcga
      361 tcgcaagctc acggctgcgc cggctccccg cagccgacgc gacgagcagg gcggcgagcg
      421 tcggggcgac gagatccagg ctgcccggcg cgaatggcag gaggacgatg gtccccagga
      481 cgagacgcca ccactggcct cgcgtccccg ttgccgccgc cggcagcatc aggccggcga
      541 gccgctcttg atgtacggga tcagggccag ctggcgcgcg cgcttgatgg ccgtcgcgag
      601 ctgccgttga tggcgcgcgc acatcccgga tagacgcgag ggcagaatct tgccgcgttc
      661 ggtcaggaac cggcccaggg tccggtcgtc cttgtagtcg aggacgcgga cgcccgactc
      721 gcacacgggg cacgtcttgg cggggcgtct catgcttcct cctcctcgag ctccagctcc
      781 tcggcgacca cgggcggcgt ggtgggcatc ggcttgggtg gctcgccctc gttcaggacg
      841 atcaggtagc gcaacaccga ctcttcgagc ttcaccgccc gctcgtactc gggcagcagg
      901 tcccccgtcg cctcgaactg ggtgacgacg tagtagccgg tttccttctt cttgatgggg
      961 taggcgaggg tgcgcttgcc ccagtggttg gaacccgaca cggtgccgct cccgctcttg
     1021 gtcaggaggg catggaaccg ctcgagcttc tcgttgatcg cctgttcttc cagagcacta
     1081 tcgaagatgt agacggtctc gtagcgacgc gtcatgaaca cctccctttg gtcgtggacg
     1141 acccggcact ggtgtggcgc cgagtagggg atatgtgagc ccgcaagcta gcggggaggg
     1201 tgcgggtcgg ccacccccgt ccttgcccca ccggtcgggg acgatagagt gtatgaagag
     1261 ggaccggagg tacccatgcg cctgactttc ctcgccgtcg ccctgtgggc gataccgatc
     1321 gcgctcggtg cacagcagga ttccgcccgc ctgacgggca cagtcacatc gctctccggg
     1381 gcgccacttt ccggggtgct gctctccctg ccggaggagg gctggtcgca caccaccgat
     1441 tcgaccggcc gattcgagca cctgaccacc aggcccgggc ggcgcacgct gcgggtggcg
     1501 gtgatcggga tcgcgccgca ggagtacgac gtccagctcc atgccgggcg cacgacggcc
     1561 atcaccgtgc tggtggatcc ggccgccatc gagctcgagc cgatcgtcgt gatcggggag
     1621 cacgaagaag ggtcattgag aatcgacggg ttctacgcgc gcaagctgcg cgctgtgtac
     1681 ggcgggctgt acttcacgca cgcggacatc atgcggcgtg ctccggacag gctcagcacc
     1741 ctgcttgggg cgacgggtct caccatgcgc tgcggcctgg tcggcggggg ttgcgtcccg
     1801 gtcgcccgat gcatcgtgcc ggtgtacctc gatggcatgc gagttcccag ccgcgagctg
     1861 gatcgcatcc cgccgagcat ggtggcaggc gtcgaggtgt atcgcagcag cttcgacacg
     1921 cctctcgagt acgcaatcca gggcggcagc tgcggcgcca tcctggtctg gacgggagtt
     1981 tagcggcagc gctggcgccc gccggtgggt gcgggctatg attcaacgc
//
LOCUS       DBNGZW010000224         3103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1862518, whole genome shotgun sequence.
ACCESSION   DBNGZW010000224 DBNGZW010000000
VERSION     DBNGZW010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3103
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1862518"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            222..722
                     /locus_tag="AC16JC_05325"
     CDS             222..722
                     /locus_tag="AC16JC_05325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164152.1"
                     /translation="MDNAAVLFLLRAIHIVGGVLWVGGVVLLTFFLLPATQALGPAAQ
                     PVMQFIMGRRKLSIYLMVLGILTTLAGLLLMARNVSNTNGAWARSPMGIGMSVGAVSA
                     ILALIVGLVVNMPAAKRLGPPRPGAAPLTDEQRAALMGRIAIGSRIVLVLLVITALVM
                     ATARYY"
     gene            917..1408
                     /locus_tag="AC16JC_05330"
     CDS             917..1408
                     /locus_tag="AC16JC_05330"
                     /inference="COORDINATES: protein motif:HMM:NF019430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR5164153.1"
                     /translation="MRKLALVVALVLGPALHAQAPGPGDGPGPNPGRGVRPSGPPPFA
                     QGPDFGRDLRPRGGPAGDIGSFLLAHTAELKLTDQQVTRLAAIARRAGERRQAAMRAM
                     DSLATRGMRRDSAGRSPGGPPPALRAEAERMRDQRHAELKEALGVLTPDQQATAWEIG
                     RRF"
     gene            1618..2076
                     /locus_tag="AC16JC_05335"
     CDS             1618..2076
                     /locus_tag="AC16JC_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR5164154.1"
                     /translation="MNHTLSDSAPGCAVRSGPDEGALVAVLGTAEELERRLEAALVAV
                     GLSVSKFDAMEQLIRAGEPMTLGCLAGQLHCVRSNVTQLIDRLEAEGLVKRGSCTEDR
                     RSVRAKVTPLGLERHAAGLEAIRSVQREVAQRLGPVQRAQLMELLSALAD"
     gene            2249..3082
                     /locus_tag="AC16JC_05340"
     CDS             2249..3082
                     /locus_tag="AC16JC_05340"
                     /inference="COORDINATES: protein motif:HMM:NF022750.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HIR5164155.1"
                     /translation="MSAPLRVSLQRVIALAFVASATIAPLRLSAQAPMLASSTPVTTV
                     SGRAAVPYGVGEELTYKATFGGIPAGTARMRVDGIEVVRGRPAYHVVFTIDGGVPFFR
                     VHDRYDSWIDVQTLASLRHRQQISEGRYKRTTTYEIFPDRGTYEEGDSVHASVAHPLD
                     DGSFIYAIRSARVRVGETRRDDRYFRPDRNPVVLTGLRQEAVRVGAGVFAATVVQPSI
                     KANGIFSENGEAQVWFSEDARRYPVLLKSRFAKFSLTLSLQSVVPGEAPVLPVVTAAA
                     P"
BASE COUNT          494 a         1066 c         1030 g          513 t
ORIGIN      
        1 gctcgggggt cggacgctcc cgaatctcca gcacctccgg cccgccaggg cgggtgatca
       61 cgacggcacg catgctcagc gtgtcgattg catgtcaggt ttcacgtcgc gtcgagctgc
      121 tggtacaccg gcacctggag actgagtata gtaccccgaa acggcggcgc cgaggcgctc
      181 ggtgcaggtc cgtcccaccg aaccaaccgg gagagcgaac gatggacaat gcggccgtgc
      241 tgttcctgct gcgggcgatt cacatcgtgg gcggcgtgct gtgggtcgga ggcgtggtgc
      301 tcctcacctt cttcctcctt ccagccacgc aggccctggg cccggcagca cagccggtga
      361 tgcagttcat catgggacgc cggaagctct ccatctatct gatggtgctg ggaatcctca
      421 ccacgctggc cgggctgctc ctcatggcgc gcaacgtgtc caacaccaat ggcgcatggg
      481 cacgttcgcc catggggatc ggcatgtccg tcggcgccgt ctcggccatt ctcgcgctga
      541 tcgtggggct ggtggtcaac atgccggcgg ccaaacggct cgggccaccc aggcccggcg
      601 ccgcgccgct cacggacgag cagcgtgcgg cgctcatggg tcgcatcgca atcggatcgc
      661 gcatcgtgct cgtgctgctc gtcatcacgg cgctcgtcat ggccaccgcg cgctactact
      721 gacactggtt tcgatccgcc gcccgcgcga cgcctggacc gagtcgcgcc gcctgcgacg
      781 aatcgcgcgg ctggatctcc atcacgtggc gcgagcggac ggcccattcc agaggaaggg
      841 cgatcggatc gtcgcgtgtc tcacaagatc ataggtgcaa cgactcagac gtccgccatc
      901 ttcccaggag accgatatgc ggaaactcgc gctcgtcgtc gcgctcgtgc tcgggccggc
      961 gctacacgct caggcaccag ggccaggaga cggccccggc ccgaatccgg gtcgcggcgt
     1021 ccgcccgtcc ggcccacctc ccttcgcgca gggaccggac ttcggccgcg acctgcgtcc
     1081 acggggcgga cccgcgggcg acatcggctc cttcctcctc gcacatacgg cggagctgaa
     1141 gctgaccgac cagcaggtga ccaggctggc agccatcgcg cgccgggcgg gagagcggcg
     1201 ccaggccgcg atgcgggcga tggactcgct ggccacgagg gggatgcgtc gcgacagcgc
     1261 cggccgcagc cccggcggcc cgccccccgc cctccgcgcc gaggccgagc gaatgcgcga
     1321 ccagcgccat gccgagctga aggaagcgct cggcgtcctg accccggatc agcaggcgac
     1381 ggcgtgggag atcgggagga ggttctgaga accgccactg cgagtatccg gcacgagtga
     1441 cgtagcgagt aaccggtatc ggtgggcgca attttcaacc ctgaacgctc cgtcgttccc
     1501 agcggctgac gggacttccg cgagcatcca gccggtactg ggtagccgct acgtctgtcg
     1561 tagcaagtac tgtcctcgca gttgcctgca gtatcttacc agcatatatt tcagtacatg
     1621 aaccacacgc tatccgactc cgctcccggt tgcgcggtcc gatccggtcc cgacgagggc
     1681 gccctggtcg ccgtgctcgg cacggccgag gagctcgagc gtcggctcga ggcggcgctg
     1741 gtggcggtcg gactctcggt gtcgaagttc gacgcgatgg agcagctcat ccgcgcgggg
     1801 gagccgatga cactgggctg tttggccggt cagctgcact gtgttcggtc caacgtcacg
     1861 cagctcatcg atcgactcga ggccgaaggg ctcgtgaagc ggggaagctg caccgaggac
     1921 cgtcggtccg tgcgtgcgaa agtcaccccg cttggtctcg agcgacacgc cgctgggctc
     1981 gaggcgatcc gatccgtgca gcgcgaggta gcgcagcggc tcggtccggt tcagcgcgcg
     2041 cagttgatgg agcttctgtc ggctctcgcc gactgacgaa ctttttattt gacccggtat
     2101 ttcatatatg aactttacag ccgccaaaac atccgacggt tgtaagcccc gagtaagtgc
     2161 cgatggcgat gcttcgcggg gttcaacgag tgacgctcga cggaaatccg ttccggccgc
     2221 agcgtcgtag aacgacccag gaaccaccat gtcagcacca ctgagagtct ccctgcagcg
     2281 cgtcatcgcg ctcgccttcg tcgcctcggc gaccatagct ccgctccgac tgtcggctca
     2341 ggcgcccatg ctggcgtcct cgacacccgt gacgaccgtc tccggccgag cggccgttcc
     2401 ctacggcgtg ggcgaggagc tcacttacaa ggccacgttc ggcggcatcc ccgcgggcac
     2461 ggcccgcatg cgcgtcgacg ggatcgaggt cgttcgcggc cgcccggcgt accacgtcgt
     2521 cttcaccatc gacggcggcg ttccattctt ccgcgtgcac gaccggtacg acagctggat
     2581 cgacgtgcag acactcgcat cgctgcggca tcgccagcag atcagcgagg gacgctacaa
     2641 gcggacgacg acgtacgaga tcttccccga ccgcggcacc tacgaggaag gcgactcggt
     2701 gcacgccagc gtcgcgcacc cgctcgacga cggctcgttc atctacgcca tccggagcgc
     2761 ccgcgtgcgc gtcggcgaga cccgccgcga cgaccgatat ttccgccccg atcggaaccc
     2821 ggtcgtgttg acggggctac gccaggaggc ggtgcgcgtc ggcgctggcg tcttcgccgc
     2881 caccgtggtg cagccgtcga tcaaggcgaa cggcatcttc tcggagaacg gcgaagcgca
     2941 ggtctggttc agcgaggacg cgcgtcgcta tccggtgctc ctcaagagca ggttcgcgaa
     3001 attctcgctc acattgtcgc tccagtcggt cgttccgggt gaggcgcccg tgctgccggt
     3061 ggtcacggcg gccgcgccgt gaggcgctgg cgtccgagca cgc
//
LOCUS       DBNGZW010000225         9547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1180948, whole genome shotgun sequence.
ACCESSION   DBNGZW010000225 DBNGZW010000000
VERSION     DBNGZW010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 9547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9547
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1180948"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..342
                     /locus_tag="AC16JC_05345"
     CDS             <1..342
                     /locus_tag="AC16JC_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CBM96 family carbohydrate-binding protein"
                     /protein_id="HIR5164156.1"
                     /translation="GSSHGSSTALAPLFQALYDFNAEVVVSGHDHDYERFAPQDANGT
                     ANQTRGIREFVVGTGGNSLNPFGTPEPNSEVRYSDTFGVIKFTLGSSGYTWEFIPVSG
                     SFRDSGSGTCH"
     gene            638..2560
                     /locus_tag="AC16JC_05350"
     CDS             638..2560
                     /locus_tag="AC16JC_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164157.1"
                     /translation="MPHDTPTTTLENLKKEAKRWLKALRADVAEAHARLARAFPNAPR
                     IPTLRDVQHALAREHGYSGWTELTHRLPLPLDASLLRRYERVAEALVTAYRTGEPGAM
                     RTVWDYFGHMRTWDAMQRYVRLDLGRREQPEDPDELSLDEARLLVARAQGMASWPALV
                     QFVASIPPGKTNLAAKPVELLKIDAQGQRHEAGRSHDWDEVIAMLRAERLPGLDAHGE
                     MTDEQLERLSHLDHLEGLHLSGSKGLTDAGLRALARLPRLRHLDLGGVPLTDRGLAVL
                     RELPALESLGLGWTRITDAGAVHLAACWQLRSVDLMGTATGDGAVRALAGKRHLVHFK
                     SGNGVTDAGLAVLREFPAFASWQGGAPTMALTDCDAGPTYLLLRGPFTDTGLGQLAGL
                     EGLFALNLDSDQLAVTGAGLDPLRDLPHLEWLGFDARDDSMPHIAALPRLRFLMCQDT
                     VAGDDGFTALSRSRTIETIWGRRCYNLRSRGFRALAGMPALRSLSVSCQNVDDAGLSA
                     LPRFPALEELMPMDVPDEGYRHIARCARLQALVLMYCRDTGDGATEHIATLPGLKRYF
                     ASYNLITDRTPELLSGMDTLEQVTFSACSRLTNAGVARLALLPRLQEVDLGGMPRVTR
                     EVAALFPPGVRVRYSM"
     gene            2631..3554
                     /locus_tag="AC16JC_05355"
     CDS             2631..3554
                     /locus_tag="AC16JC_05355"
                     /inference="COORDINATES: protein motif:HMM:NF025616.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HIR5164158.1"
                     /translation="MQRSIVTLISLVVLGACGDRARTANRSEEAQGLVLPTTAPAAER
                     DMYEAAAPEAGDRTQPDARPAPQQRMIARTAQIRAVVSDPVATVQALTTLVEAKGGFV
                     SDSRQWRSGEQMLASLTIRVPVRDLPATLDSIRHRAIRVENESTAGEDVTEQYADLRA
                     QLTNLRATETELRALLVTVRQRTQKAADILEVFNQLAAIRQQIDRTQSRITTLEQLTD
                     LATITVELVPDALAQPLTAGRWRPLAVVRAAFRTLLSTLRTLLDAVIWLLIYLVPVVL
                     VLAVPAIGVFIGIRAVRKRMGPRKGAVPDAG"
     gene            complement(3561..4745)
                     /locus_tag="AC16JC_05360"
     CDS             complement(3561..4745)
                     /locus_tag="AC16JC_05360"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR5164159.1"
                     /translation="MGKGTIAILTGGGDVPGLNPAIRAITIRALRDGYNVVGIRRGWA
                     GLVDYVPEAKVDNSESVQPLTEAIVNRAGRTGGTFLHTSRVRPSHLPRERLPAHLTGY
                     AEPVNDVTRDVLRNLEHLGVDVLIPIGGDDTLSYAKRLHDEGVHVVAIPKTMDNDVYG
                     TDYCIGFSTCVTRTIELTHSLRTSAGSHERFLVIEVFGRYAGFTALLPTMAGAADRCV
                     IPEHPVDVERLTELLVIDRNRNPSHYAVVLVSEGARLTSQEGMSFEGEETDMFGHRKL
                     GGIGDRVGVALKDSSPKYNNGRRIDVVNQRLGYLVRSGDPDALDSIVPMAFGNLALEL
                     VMRKEFGRLVSIHRGFYDSVPITSVTSEKKVVDVAKYYNTDRLRPIYDFDGAPLFIMT
                     SD"
     gene            4819..5421
                     /locus_tag="AC16JC_05365"
     CDS             4819..5421
                     /locus_tag="AC16JC_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5164160.1"
                     /translation="MVTGLPFYIREEDPPAKREILRAALKLFSTRGLVATSIRDIARE
                     SGYTNPALYKHFAGKDALALHLFETCHARVWHTCSAALAAGTTFDQKLEGYVGQWLEL
                     VDRHPEVIGFLSDSARVLWPRANPRVRRQTMIGLARTLALHASPSGGGRGAAMKIDAA
                     AACLEGTLAELARMIAVGVVEGPATRWKSALVALFKRLAV"
     gene            5484..6146
                     /locus_tag="AC16JC_05370"
     CDS             5484..6146
                     /locus_tag="AC16JC_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR5164161.1"
                     /translation="MFAIDHAATALLLKRRYPAVPITPLLLAVQAMEFAWVALNYLGV
                     ERTTTEPVVRSVADIHLAHIPYSHSVAIPVLAALLAWFVLARGLGRAAWGRAVGLGIV
                     SHLVLDLATHAHDIVLWPGHPQPALGLGLYDAAPLAAFVVELVFGIGCWWVYRGGRGL
                     LAVIVLGNLANISLFSAAIPGPEAYLAGRPQLIVTFILVQIVVTLALVGVFARRDLPR
                     GT"
     gene            complement(6143..7384)
                     /locus_tag="AC16JC_05375"
     CDS             complement(6143..7384)
                     /locus_tag="AC16JC_05375"
                     /inference="COORDINATES: protein motif:HMM:NF042530.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5164162.1"
                     /translation="MELVEHFFRHESGRLVAALTRIFGVENLALAEDVVQDAFLRALD
                     VWKTQGVPENPSAWLMAVAKRRALDVMRRQRTAREFAPEVGRQLDAESVVDEAFAAHA
                     IRDEQLRMMFSCCDPRLAEPAKLALILNILCGFGASEIASAFLTGRAGIEKRIARGRR
                     VLASAERLFDLEDAEFTARLSTVRQALYLLFNEGYHGAGPEAAVRAELCHEALRLTAL
                     LCEYPPAATPTTYALAALLSLDAARLPTRLDAAGELSALVDQDRSRWDARLIGQGLAL
                     LERSAAGQEVTAYHVEAAIAAAHASARSLEETDWRTIVSLYDRLMDIAPSPVVALNRA
                     IALSQRDGAARGLEALQAIGDRERLSGYPFYQAALGELELRRGNREAARTHFQTAQGC
                     ARNGAERRFLEKRIRIAEALG"
     gene            complement(7392..7979)
                     /locus_tag="AC16JC_05380"
     CDS             complement(7392..7979)
                     /locus_tag="AC16JC_05380"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF013989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR5164163.1"
                     /translation="MRRIMMFNHVAADGCFTTRDGRLDWVVQEPAIDQSAVDGMPGGD
                     TILFGRRTYDMFESYWPHALDDPTPAPHGTGSSANRAMAVWINAATKIVFSRTRQDVP
                     WQHSRLLRQFDPAEVAALKQQPGKDMLIFGSGTIVSLLAQHGLIDEFHFVVSPLFLAG
                     GRPLIGGLSQNMPLRLLEVKAYPSGNVSLRYAPGS"
     gene            complement(7976..8353)
                     /locus_tag="AC16JC_05385"
     CDS             complement(7976..8353)
                     /locus_tag="AC16JC_05385"
                     /inference="COORDINATES: protein motif:HMM:NF015735.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR5164164.1"
                     /translation="MSEFVYLFRASAADRSAALGTPEQAQKSLQAWMSWIRDLEANGH
                     LKSRGQPISPDGKVVRGSKQVVTDGPYVEAKDMVLGFIVIEARDLAQAVELSRGCPML
                     AGDGSVEIRPVATAMMKDLERST"
     gene            8584..8940
                     /locus_tag="AC16JC_05390"
     CDS             8584..8940
                     /locus_tag="AC16JC_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5164165.1"
                     /translation="MAKIRLSTYINFNGRAREALEFYHKVLGGTLDLRGSRLEVDGTA
                     IVATDGHPNYPPTVGDNLAIALSGGDADRLTRVFDSLAEGGRIKGPLTSQAAGERTGY
                     LLDRFGINWIVTIDRA"
     gene            complement(8956..9309)
                     /locus_tag="AC16JC_05395"
     CDS             complement(8956..9309)
                     /locus_tag="AC16JC_05395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007809277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5164166.1"
                     /translation="MSRSRGAAALQVTEAVPVFAALGDATRLGLLGRLSADGPLSITR
                     LSEGTGVTRQAITRHLYALGDAGLVRHARRGRERVWELDAKRLEKAKRCLDLIAAQWD
                     AAADRLKAFVEDPRP"
     gene            complement(9299..>9547)
                     /locus_tag="AC16JC_05400"
     CDS             complement(9299..>9547)
                     /locus_tag="AC16JC_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007874921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5164167.1"
                     /translation="RVEPQRYFSYRWHPYPSDPKRDYSTEPTTLVEFTLEEAEEGTAL
                     TIVESGFDRIPLSRRAEAFRMNDQGWAGQIQNLARYVS"
BASE COUNT         1457 a         3409 c         3195 g         1486 t
ORIGIN      
        1 ggctcgagcc acgggagctc caccgccctg gcgccgctct ttcaggcgct ctacgacttc
       61 aacgccgaag tagtcgtgag cggccacgac cacgactacg aacggttcgc cccacaggac
      121 gcgaacggga ccgccaacca gacccgcggc atccgggagt tcgtggtcgg caccggcggg
      181 aacagcctga accccttcgg aaccccggag cccaacagcg aagtgcggta cagcgacaca
      241 ttcggggtca tcaagttcac cctgggctcg agcggctaca cctgggaatt catcccggtg
      301 tccggcagct tccgggactc gggaagcggc acatgccact agggtcgacc cgccggtggg
      361 cgactcgctg cggggatcgt tcgtcacgct gccgacgctg ggtggcggcg tcatcccccg
      421 ctgcgcggca ccttcgccgc cggccggagg cacgcagcac cctgagatcg tagttgacaa
      481 ggtcaggcgc ccaccgcgag actaaagcag cgcgcgttgg ggcgcctcct gtccgcggca
      541 agggagatca ctcccaccca atcgggttcg cagcaccatg accgaacctt cttccggcct
      601 ggaactcagc ggacttcagg cgtacgggag gaaggtcatg ccgcacgaca cgcccacgac
      661 caccctggag aacctgaaga aggaagccaa gcgctggctc aaggcgctgc gcgccgacgt
      721 cgccgaggcg cacgcccgtc tcgcgcgcgc cttccccaat gccccccgga tcccgacgct
      781 gcgcgacgtg cagcatgcgc tcgctcgcga acatgggtac tccggctgga ccgagttgac
      841 gcaccgcctg ccccttccgc tggacgcgtc cctgctgcgg cgctacgagc gggtggccga
      901 ggcgctcgtc acggcctacc ggacgggtga gcccggtgcc atgcggaccg tgtgggacta
      961 cttcggccac atgcggacct gggatgcgat gcagcgctac gtgcgtctcg atctcggccg
     1021 ccgggaacag cccgaggatc cggacgagct ttcgctcgac gaggcccgcc tcctcgtcgc
     1081 ccgcgcccag gggatggcga gctggccggc gctggtgcaa ttcgtcgcgt cgattccgcc
     1141 ggggaagacc aacctcgcgg cgaagccggt ggagttgctg aagattgacg cgcaggggca
     1201 gcggcacgag gccggacgct cgcacgactg ggacgaggtc atcgcgatgc tgcgagccga
     1261 gcgcctcccc ggactggacg cgcacggcga gatgaccgac gagcagctcg agcgcctttc
     1321 gcacctcgac cacctcgaag gccttcacct gtcgggatcc aagggcctca ccgacgcagg
     1381 cctgcgagcg ctcgcccggc tgccgcggct gcgccacctc gacctgggcg gcgtgcccct
     1441 caccgaccgc ggcctcgcag tgttgcgcga gctgccggcg ctcgagtcgc tcgggctggg
     1501 gtggacgcgc atcaccgatg cgggagcggt ccatcttgcg gcgtgctggc agctccgctc
     1561 ggtcgacttg atggggaccg cgaccggcga cggcgccgtc cgcgcgctcg ccggcaagcg
     1621 ccacctcgtg catttcaagt cgggaaacgg cgtcaccgac gccggcctgg ccgtgctgcg
     1681 ggagttcccg gccttcgcga gctggcaggg tggcgcgccc accatggcgc tcaccgattg
     1741 cgacgcgggc ccgacctatc tcctgctgcg cggcccgttc acggacacgg gcctcgggca
     1801 gctggccggc ctcgagggcc tcttcgcact gaacctcgac agcgatcagc tcgcggtgac
     1861 cggcgccggg ctggaccccc tgagggatct gccgcatctc gagtggctag ggttcgacgc
     1921 cagggacgac tcgatgcccc acatcgccgc cctgccgcga ctccgcttcc tcatgtgcca
     1981 ggatacggtc gcaggcgacg acgggttcac cgccctcagc cgctcacgga cgatcgagac
     2041 catctgggga cgacgctgct acaacctgcg cagccgggga ttccgggcgc tggccgggat
     2101 gccggcgctg cggtcgctgt cggtgagctg ccagaacgtc gatgacgcgg ggttgtcggc
     2161 gctgccgcgg ttccccgccc tggaggagct gatgccgatg gatgtgccgg acgaggggta
     2221 tcgacacatc gctcgctgcg cacggctgca ggcgctggtg ctgatgtatt gccgcgacac
     2281 gggcgacggc gcgacggagc acatcgcgac gttgccgggc ctgaagcggt acttcgcgag
     2341 ctacaacctg atcaccgacc ggacgccgga gctcctgtcg gggatggaca cgctcgagca
     2401 ggtcaccttc tcggcgtgct cccgcttgac caacgcggga gtcgcccggc tggcgctcct
     2461 gccccggttg caggaggtgg atctgggagg catgccgcgg gtcacccgcg aggtcgctgc
     2521 gctgttccct ccgggcgtcc gggtgcgcta ctcgatgtga aaacggagcg gtagccaccg
     2581 caggtccgga gcagcagttt caacgacctg acccttctcc aggggacgcc atgcagcgat
     2641 ccatcgtcac tctgatttcc ctcgtcgtgc tcggtgcgtg cggtgatcga gcgcggacag
     2701 ccaaccggtc ggaagaggcc cagggactcg tcctaccgac gacggccccc gcggccgagc
     2761 gggacatgta cgaggcagct gcgccggagg ccggtgaccg gacgcagccc gacgctcgcc
     2821 cggccccaca acagcgcatg atcgcccgca cggcgcagat ccgggcggtg gtgtcggatc
     2881 cggtcgccac ggtgcaggcc ctcaccaccc tggtcgaagc gaagggcggt ttcgtctccg
     2941 actcgcggca gtggcgctcc ggggagcaga tgctggcgag cctgacgatt cgcgtgccgg
     3001 tccgcgacct ccccgccacg ctggattcga tccgccaccg cgccatccgg gtggagaatg
     3061 agagtacggc cggcgaggac gtgaccgagc agtacgccga cctgcgcgcc cagctgacga
     3121 atctccgggc gaccgagacc gagctgcgcg cgctgctcgt aacggtgcgc cagcgcaccc
     3181 agaaggccgc cgatatcctg gaggtgttca accagctcgc cgcgatccgc cagcagatcg
     3241 accggacgca atcgcggatc acgacgctcg agcagctcac ggacctggcg acgatcacag
     3301 tggagctcgt gcccgatgca ctcgcgcagc cgctcacggc cggccgctgg cggccgctgg
     3361 cggtggtgcg ggcggcgttt cgcacactcc tcagcaccct gagaacgctg ctcgacgcgg
     3421 tgatctggct gctgatctac ctggtaccgg tcgtgctggt cctggccgtg ccggcgatcg
     3481 gcgtgttcat cggcatccgg gcggtgcgca agcggatggg accccgcaaa ggcgcggtcc
     3541 cggacgccgg ctaggagccg tcagtcactc gtcatgatga acagcggcgc gccgtcgaag
     3601 tcgtagatcg gccgcagccg gtcggtgttg tagtacttcg cgacgtccac gactttcttc
     3661 tccgacgtca cgctggtgat cggcacgctg tcgtagaacc cgcggtggat gctgaccagg
     3721 cggccgaact ccttccgcat gaccagctcg agcgcgaggt tgccgaaggc catcggcacg
     3781 atggaatcga gcgcgtcggg atcgccggag cgcacgaggt accccagccg ctggttgacg
     3841 acgtcgatgc gccggccgtt gttgtacttc ggagacgagt ccttcagggc cacgcccacc
     3901 cggtcgccga tcccgcccag cttgcggtgc ccgaacatat ccgtctcttc accctcgaag
     3961 ctcatgccct cctgcgaggt caggcgcgcg ccctccgaca cgagcacgac cgcgtagtgg
     4021 ctcggattcc ggttgcggtc gatcacgagc agctccgtca ggcgctccac gtccactgga
     4081 tgctcgggga tgacgcagcg gtcggccgcc cccgccatcg tcggcagcag ggccgtgaag
     4141 ccggcatagc gcccgaacac ctcgatcacg aggaaccgct cgtgcgagcc cgccgacgtg
     4201 cgcagcgagt gcgtcaattc gatggtgcgc gtcacgcagg tactgaagcc gatgcagtag
     4261 tcggtgccgt agacgtcgtt gtccatggtc ttcgggatgg cgacgacgtg caccccctcg
     4321 tcgtgcaggc gcttggcgta gctcagggta tcgtcgccgc cgatggggat caggacgtcc
     4381 acgccgaggt gctcgaggtt ccggagcacg tcgcgggtga cgtcgttgac cggctcggcg
     4441 tagcccgtga gatgggccgg caggcgctcg cgcggcaggt ggctgggccg cacccgggag
     4501 gtgtggagga aggtgccgcc cgtccgaccc gcgcgattga cgatggcctc ggtcagcggc
     4561 tgcacgctct cgctgttgtc gaccttcgcc tcgggcacgt agtcgacgag cccggcccag
     4621 ccgcgccgga tgccgaccac gttgtagccg tcgcgcaggg cgcgaatcgt gatggcgcgg
     4681 atggccggat tgagccccgg gacgtccccg ccgccggtga gaatggcgat tgtacccttc
     4741 cccatggatg cgctcctttg cggcgcaaga taatcccccc gcttgacagc ggggttagca
     4801 gacgctaact acggtcctat ggtcaccggc ctgccgttct acatccgcga ggaggacccc
     4861 ccggcgaagc gggagatcct gcgcgccgcg ctcaagctgt tcagcacgcg cgggctcgtg
     4921 gccacgagca tccgcgacat cgcccgggaa agcggctaca ccaacccggc gctctacaag
     4981 cacttcgccg gcaaggacgc gttggcgctg catttgttcg agacctgcca tgcccgcgtc
     5041 tggcacacct gctcggcggc gctcgcggcc ggcaccacct tcgaccagaa actcgagggg
     5101 tacgtcggcc aatggctgga gctggtggat cggcatccgg aggtcatcgg attcctgtcc
     5161 gatagcgcgc gggtcttgtg gcccagggcc aacccccgcg tgcggcgaca gacgatgatc
     5221 ggtctcgccc gcacgctggc actgcacgcc tccccctccg gggggggacg cggcgccgcg
     5281 atgaagatcg atgccgccgc ggcgtgcctc gaaggcacgc tggccgaatt ggcccggatg
     5341 atcgccgtcg gcgtcgtgga aggcccggcc acacgctgga agagcgccct ggtggcgctg
     5401 ttcaagcgtc tcgccgtctg atttttttgt ccgcgaagtt agcgttcgct aactcccgac
     5461 ccgctgccca ggagagcccg accatgttcg ccatcgatca cgcggcaacc gccctgctcc
     5521 tcaagcgccg ctatccggcg gtgccgatca ccccgctgct gctggccgtc caggccatgg
     5581 agttcgcctg ggtcgcgctc aactacctcg gagtcgagcg caccacgacc gagcccgtgg
     5641 tgcgcagcgt cgccgacatt cacctggcgc acatccccta ctcccactcc gtcgccattc
     5701 ccgtgctcgc cgccctgctc gcctggttcg tgctcgcgcg ggggttggga cgcgcggcgt
     5761 ggggccgggc cgtcgggctc ggcatcgtct cacacctggt gctggatctc gccacgcacg
     5821 cgcacgacat cgtgctctgg ccgggccatc cgcaacccgc gttgggcctg ggtttgtacg
     5881 atgcagcccc gctcgcggcc ttcgtcgtcg agctcgtgtt cggcatcggc tgttggtggg
     5941 tctatcgcgg cggccgcggc ctgctcgccg tgatcgtcct ggggaacctc gcgaatatct
     6001 ccctgttctc cgccgccatc cccggtcccg aggcgtacct ggccgggcgg ccgcagctca
     6061 tcgtgacctt cattctcgtc cagatcgtcg tcacgctggc gctcgtgggc gtgttcgccc
     6121 ggcgcgatct ccccaggggg acctaaccga gcgcctccgc gatccgtatg cgtttctcga
     6181 gaaagcgccg ctccgcgccg ttgcgggcac agccctgcgc cgtctgaaag tgcgtccgtg
     6241 cggcctcccg gttgccgcgg cgcagctcca gctctcccag cgcggcttga tagaacggat
     6301 agccggacag tcgctcgcga tccccgatcg cctgcagcgc ctcgagcccc cgggcggctc
     6361 cgtcgcgctg gctcagggcg atggcgcgat tgagcgccac caccggcgat ggggcgatgt
     6421 ccatcaaccg gtcgtacagc gagacgatcg tgcgccagtc cgtttcctcg aggctgcgcg
     6481 cgctcgcatg cgcggcggcg atggccgcct cgacgtggta ggccgtcacc tcctgcccgg
     6541 cggcggagcg ctcgagcagg gcgagcccct gcccgatgag ccgggcgtcc cagcgcgagc
     6601 gatcctggtc cacgagggcg ctcagctcgc ccgccgcgtc gagccgcgtc ggcagccgcg
     6661 ccgcgtccag cgacaggagc gccgccaggg cgtacgtggt gggggtcgcc gccggtggat
     6721 actcgcacag cagcgccgtc agccgcaggg cttcatggca cagctccgcc cgcaccgccg
     6781 cctcgggacc cgcaccgtga tacccttcgt tgaacagcag gtacagcgcc tgccggacgg
     6841 tcgagagccg cgccgtgaat tcggcatcct ccagatcgaa cagccgctcg gcgcttgcga
     6901 gcacccgcct tccgcgcgcg atgcgcttct cgatgccggc acggcccgtg aggaacgcgc
     6961 tcgcgatctc gctcgcgccg aagccgcaga ggatgttgag gatgagggcg agcttcgcgg
     7021 gttccgccaa ccgcggatcg caacaggaga acatcatccg cagctgctcg tcccggatcg
     7081 cgtgcgccgc gaaggcctcg tccacgaccg actcggcgtc gagctggcgc ccgacctcgg
     7141 gcgcgaactc gcgcgcggtg cgctgccgcc gcatgacgtc gagcgcccgg cgcttcgcca
     7201 ccgccatgag ccaggccgac gggttctccg gaacaccctg cgtcttccag acgtccagcg
     7261 ctcgaaggaa cgcgtcctgc accacgtcct cggccagcgc caggttctcc accccgaaga
     7321 tccgcgtgag cgccgcgacc agacgccccg actcgtggcg gaagaaatgc tcgaccagct
     7381 ccacccggac ctcaactccc cggcgcgtag cgcaagctga cgttgcccga ggggtaggct
     7441 ttgacctcga gcagccggag cggcatgttc tgcgacaggc ccccgatcaa tggccggccg
     7501 cccgccagga acagcgggct gaccacgaaa tggaattcgt cgatcagccc gtgctgcgcc
     7561 agcagcgata cgatggtgcc gctcccgaag atcaacatgt ccttgcccgg ctgctgcttc
     7621 agggccgcga cctcggcagg gtcgaactgc cgcagcagcc gggagtgctg ccacggcacg
     7681 tcctgccgcg ttctcgaaaa gacgatcttg gtcgcggcgt tgatccagac ggccatggcg
     7741 cgattcgcgg acgacccggt cccatggggc gcgggggtcg ggtcgtcgag ggcgtgcggc
     7801 cagtaggact cgaacatgtc gtaggtccgg cgcccgaaca gaatcgtgtc gccacccggc
     7861 atgccgtcga cggccgactg atcgatcgcc ggttcctgca ccacccagtc gagccgcccg
     7921 tcgcgcgtcg tgaagcatcc gtcggcggcc acgtgattga acatcatgat acgtctcatg
     7981 tcgacctctc gagatccttc atcatggccg ttgccacggg acggatctcc accgagccgt
     8041 cgccggcgag catcgggcag ccgcgcgaca gctcgaccgc ctgcgccaag tcccgggcct
     8101 cgatgacgat gaaccccagg accatgtcct tggcctcgac gtacggaccg tcggtgacga
     8161 cctgcttgct gccccgcacc accttcccgt cgggggaaat cggctgcccc cggctcttca
     8221 ggtgaccgtt cgcttccaag tcccggatcc acgacatcca cgcctgcagg ctcttctggg
     8281 cctgctccgg cgttcccagg gccgcgctcc ggtccgccgc gctggcccga aacagataga
     8341 cgaactcgct catggtgccg cctcctggag tgtgacgtcc ttcgacctcg tcgacaatga
     8401 cgttcgaagg agcggccacc ggacattccg ggcgcccgga ccggctgagg cgtacccatg
     8461 cctccgggac cgcgtcgtcg gcatcgcatc cacggtcaca ttccttcagt tcgccccgtc
     8521 atggcggtaa cctcatggga gagacgattc gacccggtgg accgtgacga gaggagccct
     8581 gtgatggcca agatccggct gagcacctac atcaacttca atggccgcgc ccgcgaggcc
     8641 ctggaattct atcacaaggt actgggcgga acgctcgacc tgcgcgggtc acggctcgag
     8701 gtggacggaa cggcgatcgt ggcgacggac ggccacccga actacccccc taccgtaggc
     8761 gacaacctgg ccattgccct gagcggcggc gatgcggatc gcctcacccg ggtgttcgac
     8821 agcctcgctg aaggcggcag aatcaagggt cccctgacct cccaggcagc gggcgagcgg
     8881 acggggtatc tcctcgaccg attcggcatc aactggatcg tgacgatcga ccgggcctag
     8941 cgcccggcgt cgacgtcaag gccgtggatc ctccacgaaa gccttcaatc ggtcggccgc
     9001 cgcgtcccac tgcgcggcga tgagatcgag acatcgcttc gccttctcca ggcgcttggc
     9061 gtccagctcc cagacccgtt cccgcccgcg gcgggcatgg cgcacgagcc cggcatcccc
     9121 cagggcgtag agatgccggg tgatcgcctg ccgcgtcacg ccggtgccct cgctcaggcg
     9181 cgtgatcgag agcggcccgt cggccgacag gcgccccagc aggccgagcc gggtcgcgtc
     9241 acccagggcg gcgaacaccg gcacggcctc ggtcacctgc aacgccgctg cgccgcggct
     9301 acgagacata gcgcgccagg ttctggattt ggccggccca cccctgatcg ttcatccgga
     9361 acgcctcggc ccggcgggag agcgggatcc gatcgaaccc cgattcaacg atggtcaggg
     9421 cggtgccctc ctccgcctcc tcgagcgtga actcgaccag cgtggtcggc tcggtcgagt
     9481 agtctctctt ggggtcgctc gggtacgggt gccaacgata cgagaagtac cgttgcggct
     9541 cgacgcg
//
LOCUS       DBNGZW010000226         2159 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_224821, whole genome shotgun sequence.
ACCESSION   DBNGZW010000226 DBNGZW010000000
VERSION     DBNGZW010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_224821"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="AC16JC_05405"
     CDS             complement(<1..273)
                     /locus_tag="AC16JC_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164168.1"
                     /translation="MTDQEFDALVARLQRQSAAHPVIYRMRVLLLALLGYGYLLGAVL
                     FLGIGIALIIGAAVALRVAFFAGRIVGPLATQMSTVLRALWVRIPTP"
     gene            complement(419..1366)
                     /locus_tag="AC16JC_05410"
     CDS             complement(419..1366)
                     /locus_tag="AC16JC_05410"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5164169.1"
                     /translation="MKPPIMAFCVACAVGSSSAQAPAWQPSPGHTQVPIWPGVPPHAR
                     QAPGAEAVTTRMNDHLVAGRPWTYISNVSRPTLTVYSPPGSNTGAAVVVFPGGGYQIL
                     AIDLEGTEVCDWLTARGITCVLLKYRVPNAGPSWDQQCGCDLDTRSSMPLEDAQRTVG
                     LVRFHAAEWHIDPPKIGVLGFSAGGHLVAALSTRFDRRVYPAVDAADRESCRPDFAVV
                     LYPGHLYDPARSGLNADIHVSRNTPATFLLQAQNDSVDGVSNSLVYCTALKNAGVPVD
                     LHLYAEGGHAFGLRRTRSPITAWPRLVETWLQSIGMISP"
     gene            complement(1415..>2159)
                     /locus_tag="AC16JC_05415"
     CDS             complement(1415..>2159)
                     /locus_tag="AC16JC_05415"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR5164170.1"
                     /translation="LAQMAAHDLDVRGRDWALRQLATVHDSTAQAARRFVVLSEHLAE
                     LRQLALEAMGGDSSDATRAVLRAALRDPDGSVRGAALNALAALDQPGAAAAATRIYAS
                     DPSDAARQAALGVVARAQAPEALTLLVAASAADQPLGTRFTAISYLRRMHDPKALDAL
                     EQLTATTEDRNIRTSGLGALAANDSARATAVALRLIADPDPLFASSAVRVAARVGGAA
                     AKARLQQALAQEKRVFVRLAIQQALAPRQ"
BASE COUNT          336 a          774 c          743 g          306 t
ORIGIN      
        1 gggcgtcggg atgcggaccc agagggctct gagtaccgtg ctcatctggg tggcgagcgg
       61 tccgacgatc ctgccggcga agaacgccac ccgcaacgcg accgcggcgc cgatgatgag
      121 ggcgatcccg atgcccagga acaggaccgc gcccagcagg tagccgtacc ccaggagggc
      181 gaggagcagg acacgcatcc ggtagatcac cggatgtgcg gcggactgcc gctgcaatct
      241 cgcgacgagg gcgtcgaatt cttgatcggt catcgccggg ccctcgacac aatgtcgagc
      301 gcccgacaag cgatcgtcaa ttccttgaga ggacctcttc gaccgtgggg tcagcccccc
      361 ggcgtggggg cggcggctcg ggcgacgtac agccctcggc ccgagggctg ccggtctcct
      421 acggcgaaat catgccgatc gactgcagcc acgtttcgac caaccgcggc caggctgtaa
      481 tcggagacct cgttcgccgc aacccgaagg cgtgcccccc ctccgcgtac agatgcaggt
      541 ccacgggtac gccggcgttc ttcagcgccg tgcagtaaac gagtgagttg ctcacgccgt
      601 ccacgctgtc attctgggcc tgcagcaaga aggtcgccgg cgtgttgcgg ctcacatgaa
      661 tatccgcatt cagcccggac ctggcggggt catacagatg tccgggatac aacaccaccg
      721 cgaaatccgg acggcagctt tcccggtcgg cggcgtccac cgccggatac acccggcggt
      781 cgaaacgcgt gctcagggcg gccaccagat gcccacccgc tgagaatccg agcacgccga
      841 tcttgggtgg atcgatgtgc cactccgccg cgtgaaagcg caccagcccg accgtccgct
      901 gcgcgtcttc gagcggcatg gacgatctcg tatcgaggtc acaaccgcac tgctgatccc
      961 aggacggccc ggcattgggc acgcggtatt tcagcagcac gcacgtgatc cccctggcgg
     1021 tcagccagtc gcagacttcg gtgccttcga gatcgatggc caggatctga tagccgccgc
     1081 cgggaaacac gacgaccgcc gcgccggtat tgctccccgg tggcgaatag acggtgaggg
     1141 tgggccgcga gacgttgctg atgtacgtcc acggcctgcc ggcgaccagg tggtcgttca
     1201 tcctcgtcgt gacagcttcc gcccctgggg cttgccgcgc atggggcggc actccaggcc
     1261 agatcggcac ctgggtgtgt ccgggcgacg gctgccaggc cggcgcctgc gcgctcgagc
     1321 tccccacagc gcaagcgacg cagaacgcca tgatcggtgg cttcatcgtc gagcggcggg
     1381 cgcggctacc cgcccgaggc cgagaacacg ccgcctactg ccgcggcgcg agcgcctgct
     1441 ggatcgccaa ccgcacgaac acacgcttct cctgcgccag ggcctgctgc agccgcgcct
     1501 tggccgccgc accgccgacc cgcgccgcca cgcgcaccgc gctcgaggcg aacaggggat
     1561 cgggatcggc gatcaatcgc aaggccaccg cggtcgcgcg cgcgctgtcg ttggccgcga
     1621 gcgcgccgag ccccgacgtg cggatgttgc gatcctcggt ggtcgccgtc agctgctcga
     1681 gcgcgtccag ggccttgggg tcgtgcatcc gccgcagata cgagatggcc gtgaaccggg
     1741 tgccgagcgg ctgatccgct gcgctcgccg ccacgagcag ggtgagcgcc tccggcgcct
     1801 gcgcgcgcgc gacgactccc agggcggcct gtcgcgcggc gtcgctcgga tcgctggcat
     1861 agatgcgggt ggcggctgcc gccgccccgg gctggtcgag cgccgccagc gcgttcagcg
     1921 cggccccccg cacgctgccg tcgggatcgc gcagcgcggc gcgcagcacg gcccgggtgg
     1981 cgtcactcga atcgccgccc atcgcctcga gcgccagctg gcgcagctcg gccaggtgct
     2041 cgctcagcac gacgaagcgc cgcgccgcct gagccgtgga gtcatggacg gtcgcgagct
     2101 ggcgcagcgc ccaatcccgc ccgcgcacgt cgaggtcgtg cgcggccatc tgcgcgagc
//
LOCUS       DBNGZW010000227         4146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_157479, whole genome shotgun sequence.
ACCESSION   DBNGZW010000227 DBNGZW010000000
VERSION     DBNGZW010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_157479"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1225)
                     /locus_tag="AC16JC_05420"
     CDS             complement(<1..1225)
                     /locus_tag="AC16JC_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HIR5164171.1"
                     /translation="MGLDSLRAFIGAIDAAGDLRRVDRPVSVTLEITEVADRVMKSAG
                     GGPALLFERPTLAHGGASSYPVAVNLFGSERRMALALGVQRLDEIGGRIADMLNVKVP
                     EGLLAKLALLPRLAEMAKFPPRVIGDRAQCQEIVVRGEEVDLERFPVPVCWPQDGGPY
                     ITLPGVITRDPVSGTRNVGMYRIQVTGARTVAMHWQRHKVGAAHWRAMAERGETMPVA
                     IALGGDPASIYAASAPLPPSIDEYLFAGFLRRAPVHLVKALTSDLEVPAEAEIVLEGY
                     VDPREPLVLEGPFGDHTGFYSLADYYPQVHITAVTMRANPIWPHTIVGRPPMEDYWLG
                     HATERIFLPLLKLTLPEVVDYHMPAEGIFHNLVFVSIDKQYPGQAYKVMNGLWGQGLM
                     SLAKVIVVVDRDVNVR"
     gene            complement(1212..1955)
                     /locus_tag="AC16JC_05425"
     CDS             complement(1212..1955)
                     /locus_tag="AC16JC_05425"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019010576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HIR5164172.1"
                     /translation="MAAPLPSDVHSPLSPPVAGQTKRSYVRAMFTAIAPRYDLLNHLL
                     SLNVDRRWRRIAVARLGWEARPDGTYLDLCAGTMDLAAVLAGRPGFRGRVIGADFVVP
                     MLARGRGKSSRAAPVGADALALPFPAASFDGAMVAFGVRNLADLDAGLAEASRVLKPG
                     ARFVVLEFTTPRFAPLRAAYLFYFQRVLPLIGRAVSKHTDAYSYLPESVLAFPDPDAL
                     AARLRAAGFHDVRYELLTGGICAVHHGAR"
     gene            2008..2565
                     /locus_tag="AC16JC_05430"
     CDS             2008..2565
                     /locus_tag="AC16JC_05430"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5164173.1"
                     /translation="MEWAKDGHEAAFRELVRRYERPVFSLVYRMVRDRALAEDLAQET
                     FIKVLNGIRSYRPEFKFSSWIFKIANNAAIDHLRRRELDTLSLDGSPHAQTAEEMHAT
                     ALQIGGRQESPLDEVASRELGSQIELAIARLRPEYRACILLRHVEGHSYEEIAQILDL
                     PLGTVKTYIHRGRNELRVYLEAVHE"
     gene            2599..2847
                     /locus_tag="AC16JC_05435"
     CDS             2599..2847
                     /locus_tag="AC16JC_05435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164174.1"
                     /translation="MSEHSHNPHLTTDEIELWALGLLPAARAIHLAECGECLTTAEHE
                     RKLQRDLVQLPRFGPEYGFVERVMAKVRIPTPSGDIRP"
     gene            complement(2923..>4146)
                     /locus_tag="AC16JC_05440"
     CDS             complement(2923..>4146)
                     /locus_tag="AC16JC_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5164175.1"
                     /translation="ATEITQRKELEGQLRQAQKMEAVGQLAGGIAHDFNNVLTAVLGY
                     TRLLLKDATLPAVALGDVQEIEQAARRAASLTQQLLAFSRKQMLQPEILDVNEVVQGM
                     RSLLRRLIGEHITLVATPDPRPMLVRADQGQLQQVIINLSVNARDAMPAGGTLTIATG
                     AVELGPSFVAAHPGSQPGPHILLQVTDTGMGMDAETRGRVFEPFFTTKAVGKGSGLGL
                     ATVYGIVKQSGGYIDVSSEPTRGSVFTVYLARVMEGAPVAPEPAPAEPPRAGGETVLL
                     VEDEPGVRSLACRALRRFGYRVLEAGNGAEALKVARQSQDPIHLLLTDVVMPEMGGRE
                     LALMLKRERPATRVLYTSGYPDAAGVQEEVREAVVAYLPKPYTPEDLAKKVREVLAAP
                     GGGEPVAPGGGGHRQ"
BASE COUNT          652 a         1461 c         1426 g          607 t
ORIGIN      
        1 cgcgcacgtt cacgtcccgg tccaccacga cgatgacctt ggcgagggac atcaggccct
       61 gcccccacag gccgttcatc accttgtagg cctggccggg atactgcttg tcgatgctca
      121 cgaagacgag gttgtggaag atgccctcgg ccggcatgtg gtagtccacg acctcgggaa
      181 gcgtgagctt cagcagcggg aggaagatcc gctccgtggc gtggccgagc cagtaatcct
      241 ccatgggcgg ccgccccacg atggtgtgcg gccagatggg attggcgcgc atcgtgaccg
      301 cggtgatgtg gacctgcgga tagtagtcgg cgagcgagta gaacccggtg tgatcgccga
      361 acggcccctc gagcaccagc ggctcgcgcg ggtccacgta tccctcgagc acgatctccg
      421 cttcggccgg aacctcgaga tcgctcgtca gggccttgac caggtggacc ggcgcccggc
      481 gcaggaagcc ggcgaacaag tactcgtcga tcgacggcgg tagcggcgcg gaggcggcgt
      541 agatgctggc gggatccccg ccgagcgcga tcgccacggg catcgtctct ccccgctcgg
      601 ccatcgcgcg ccagtgcgcc gcgcccacct tgtgccgctg ccagtgcatc gccacggtac
      661 gagccccggt gacctggata cggtacatcc cgacgttacg cgtcccgctc accgggtcgc
      721 gggtgatcac gcccggcagg gtgatgtagg gcccgccgtc ctgcggccag cacaccggca
      781 ccgggaaccg ctccaagtcc acctcctcgc cgcgcaccac gatctcctgg cactgcgcac
      841 ggtcgccgat cacgcggggc gggaacttcg ccatttcggc gaggcgcggc agcagggcga
      901 gcttcgcgag cagtccctcg ggcaccttga cgttcagcat gtcggcgata cgcccgccga
      961 tctcgtcgag ccgctggacg cccaaggcga gcgccatgcg ccgctcggac ccgaacaggt
     1021 tcaccgcgac gggatacgag gacgccccac cgtgcgccag cgtgggccgt tcgaacagga
     1081 gggccgggcc gccgcccgcc gatttcatca cgcgatcggc gacctcggtg atctccaggg
     1141 tgaccgacac gggccggtcc acgcgccgca ggtcgcccgc ggcgtcgatc gcgccgatga
     1201 aggcgcggag gctatcgagc cccatggtgg accgcgcaaa tgccgccggt cagcagctca
     1261 tagcgaacgt cgtggaagcc cgccgcgcgg agccgcgcgg cgagcgcatc gggatcgggg
     1321 aacgcgagca cggactctgg caggtaggag taggcgtccg tatgtttcga caccgcgcga
     1381 ccgatgagcg gcaggacgcg ctgaaagtag aacaggtagg cggcccgcag gggagcgaag
     1441 cgcggcgtcg tgaattccag cacgacgaac cgtgcgcccg gcttgagcac ccggctcgct
     1501 tccgcgagtc ccgcgtccag gtcggcgagg ttgcgcaccc cgaaggcgac catcgccccg
     1561 tcgaacgacg cggcgggaaa cggcagggcc agcgcatcgg ctccgaccgg cgcggcgcgc
     1621 gacgacttgc cgcggccgcg ggcgagcatc ggcacgacga aatcggcgcc gatcacgcgg
     1681 ccgcggaacc cggggcggcc ggccagcacc gccgccagat ccatcgtgcc ggcgcacaga
     1741 tccaggtagg ttccgtccgg gcgggcttcc catcccaggc gcgcgaccgc gatccgtcgc
     1801 cagcggcggt ccacgttgag gctgaggaga tggttgagga gatcgtagcg cggcgcgatg
     1861 gccgtgaaca tggcgcgcac gtaggaccgt ttggtctggc cggcgacggg gggagagagg
     1921 ggcgaatgca catcggacgg aaggggcgcc gccataggcg cgaaagatag gccgggcaac
     1981 ctcaccggcg ccagcgacca gcaggtggtg gagtgggcga aggacgggca cgaggcggcg
     2041 tttcgcgagc tggtgcgccg ctacgagcgt cccgtcttct cgctcgtcta ccgcatggtc
     2101 cgggaccgcg cgctcgcgga ggatctggcg caggaaacgt tcatcaaggt gctgaacggg
     2161 atccgctcct atcggccgga gttcaagttc tcgtcctgga ttttcaagat cgccaacaac
     2221 gcggcgatcg atcatctgcg ccgtcgcgag ctcgacacgc tgtcgctgga cggctcaccc
     2281 catgcgcaga cggcggaaga gatgcacgcc accgccctgc agatcggcgg ccggcaggag
     2341 tccccgctcg acgaggtggc gtcgcgcgaa ctggggagtc agatcgagct ggccatcgcg
     2401 cggctgcgtc ccgagtatcg ggcctgcatc ttgctccgcc acgtcgaagg acacagctac
     2461 gaggagatcg cccagattct cgatctgccc ctcggcactg ttaagaccta catccaccgg
     2521 ggccgtaacg agttacgtgt gtacctcgaa gcagtgcacg aatgaaacca ctcgccgccg
     2581 agcgccgtag gggccgccat gtctgaacat tcccacaatc cgcacctcac cacggacgaa
     2641 atcgagctgt gggccctggg gctcttgccg gccgcgcggg ccattcacct cgccgagtgc
     2701 ggcgagtgcc tcaccaccgc ggagcacgag cgcaagctgc agcgcgatct ggtgcagctg
     2761 ccgcgtttcg gtccagagta cggcttcgtc gaacgggtga tggccaaggt gcggatcccg
     2821 actccgtcgg gcgacatcag gccctgaccc gcgcgacatt ccgagcgggc ggtggggggc
     2881 ggtcgatgcg accctccgcc gcccgttttg cgtccggcgc cctcactgcc tgtgtccgcc
     2941 gccccccggt gccaccggct cccctcctcc gggcgcggcc aggacctccc ggactttctt
     3001 cgccaggtcc tccggcgtgt atggcttcgg cagatacgcc accaccgcct cccgcacctc
     3061 ttcctgcaca ccggcggcgt ccggataacc cgaggtgtac agcacccgcg tcgcggggcg
     3121 ctcgcgtttc agcatcagcg ccaactcgcg gccacccatc tcgggcatca cgacgtcggt
     3181 gagcaggagg tggatcgggt cctgtgactg ccgggccacc ttcagcgctt ccgccccgtt
     3241 gccggcctcg aggacgcgat agccgaagcg ccgcagggcg cgacacgcca ggctgcgcac
     3301 gcccggctcg tcctccacga ggagcacggt ttcgcctccg gctcgcggcg gctcggccgg
     3361 cgccggctcc ggcgccacgg gcgcgccctc catgacgcgg gccaggtaca cggtgaagac
     3421 gctgcctcgc gtgggctcgc tcgagacgtc gatgtacccg ccgctctgct tcacgatgcc
     3481 gtacacggtc gcgagaccca agccgctccc cttgccgacc gccttggtgg tgaagaacgg
     3541 ttcgaacacc cggccgcggg tctccgcatc cattcccatc ccggtgtcgg tcacctgcag
     3601 caggatgtgc ggcccgggct gcgaacccgg atgggcagcg acgaagctcg ggcccagttc
     3661 cacggcgccg gtcgcgatcg tgagcgtgcc gcccgccggc atcgcatccc gggcgttcac
     3721 ggagagattg atgatgacct gctgcagctg gccctggtcg gcccgcacga gcatgggccg
     3781 cgggtcgggc gtggcgacga gggtgatgtg ctcgccgatg aggcgccgca acagggagcg
     3841 catcccctgc accacctcgt tgacgtcgag gatctccggc tggagcatct gcttccggct
     3901 gaacgccagc agctgctggg tgagcgacgc cgcccgccgg gcggcctgct cgatctcctg
     3961 gacgtcgccg agcgccaccg ccggtaacgt cgcgtccttg agcaggagcc gggtgtaacc
     4021 gagcaccgcc gtgagcacgt tgttgaagtc gtgcgcgatg ccgcccgcca gctggcccac
     4081 cgcctccatc ttctgagcct gccgcagctg gccctcgagc tccttgcgct gcgtgatctc
     4141 ggtggc
//
LOCUS       DBNGZW010000228         1446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_20396, whole genome shotgun sequence.
ACCESSION   DBNGZW010000228 DBNGZW010000000
VERSION     DBNGZW010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_20396"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="AC16JC_05445"
     CDS             <1..977
                     /locus_tag="AC16JC_05445"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5164176.1"
                     /translation="VEPDGATFARADSMARGIGSGDMKGGDVIILYALKALRAAGALK
                     GLGLTLIFTGDEEHVGEPRTAAREALIDAARRTEVALAFEGGNRSDATVARRGASSWS
                     LTVTGHQAHSAGVFGRGAGYGAIYELARILDAFREQLVGEEYLTFNVATVVGGTEVSY
                     DTVAISGTAASKLNIVPRLAIAQGDLRFISEEQKERTRAKMREIVAQHLPGTDATITF
                     EDGYPAMSPTPGNARILAVYDGASRALGYDSVTALDPGRRGAGDISFVAPFIDGLDGL
                     GALGSGSHTPDEQVNLAALPMQTARAALLLVRLGRRRGSEFARPAEGQ"
     gene            1077..>1446
                     /locus_tag="AC16JC_05450"
     CDS             1077..>1446
                     /locus_tag="AC16JC_05450"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR5164177.1"
                     /translation="MHCLCVGLALAWQAPVQDSGASVYGQIRTSAGQPVADAEIEAWG
                     TPLRTRADAEGRFDLRGLPSGTLSLSVTGIGFEAIERRFTLRRGQRLRWNAVIPVSKW
                     EARRAQQELAIAAAGGLDSMA"
BASE COUNT          218 a          470 c          550 g          208 t
ORIGIN      
        1 tggtcgagcc cgacggggcc accttcgcgc gggcggactc gatggcgcgc ggcatcggct
       61 cgggcgacat gaagggcggc gacgtgatca tcctgtacgc gctgaaggcg ctgcgcgcgg
      121 cgggcgctct caagggcctc ggtctcacgc tgatcttcac cggcgacgag gagcacgtcg
      181 gtgagccacg gacggccgcg cgggaggcgc tgatcgatgc ggcgcggcgc accgaggtcg
      241 ccctggcgtt cgagggcggc aaccgcagcg atgcgacggt cgcccggcgt ggcgcgagct
      301 cgtggagcct gacggtcacg gggcatcagg cacattccgc cggcgtgttc ggcaggggcg
      361 ccgggtacgg cgccatctac gagctggccc gcatcctgga cgcgtttcgc gagcagctcg
      421 tgggcgagga gtatctgacc ttcaacgtcg cgacggtcgt gggcggcacg gaggtcagct
      481 acgacaccgt cgccatcagc gggacggccg ccagcaagct caacatcgtg ccgcggctcg
      541 ccatcgcgca gggcgacctg cggttcatct ccgaggagca gaaggagcgt acgagggcga
      601 agatgcgcga aatcgtggcg cagcatctcc cggggaccga cgcgacgatc accttcgagg
      661 atggctaccc ggcgatgtcc cccacgccgg gcaacgcccg gatcctggcc gtctacgacg
      721 gggcaagccg ggcgctgggc tacgactcgg tcaccgcgct cgacccgggg cgccgcggcg
      781 cgggcgatat ctcgttcgtc gcaccgttca tcgacgggct cgacgggctc ggcgcgctgg
      841 gcagcgggtc gcacacgccc gacgaacagg tcaatctggc ggcgcttccg atgcagacgg
      901 cgcgggcggc gttgttgctg gtgcggctgg ggcggcgccg ggggagcgag ttcgcccggc
      961 cggccgaggg gcagtagagg cctgtcgccg gcaaggtgtg ttcccgcacg ttttgatcgc
     1021 gtctttcttt agcagggcga atcgccgcgc aaaagaggag cgacatggtg tctctagtgc
     1081 actgcctatg cgtgggtctg gccctcgcct ggcaggctcc cgtccaggac agcggcgcat
     1141 cggtctatgg ccagatccgc accagcgcgg gccagcccgt ggccgacgcg gagatcgagg
     1201 catggggaac cccgctcagg acccgagcgg acgccgaggg tcgcttcgat ctccggggcc
     1261 tgccgtccgg cacgctgtcc ttgtcggtga ctgggatcgg cttcgaggcc atcgagcgca
     1321 gattcacgct ccgccgaggg cagcgactca ggtggaacgc cgtgatcccg gtatcgaagt
     1381 gggaagcccg gcgggcacag caggagttgg cgatagcggc ggcgggcgga ctcgacagca
     1441 tggcca
//
LOCUS       DBNGZW010000229         3072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_906412, whole genome shotgun sequence.
ACCESSION   DBNGZW010000229 DBNGZW010000000
VERSION     DBNGZW010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3072
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_906412"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="AC16JC_05455"
     CDS             complement(<1..523)
                     /locus_tag="AC16JC_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164178.1"
                     /translation="MNPVPAALLLAFALAAGFAPWRRAGWTTPVRTLLCEAGALAGTV
                     VLGWLGGRLVHPTSQGYFWGRLLLLSAAYWYACAGGADLVRRVLDLVPVSESSTSRVA
                     KGGINVPAAELSRGLLIGVLERALALTLVLVNQFGALGLILAAKALARFRALDDRDFA
                     EYVLIGTLTSMLLA"
     gene            complement(520..1146)
                     /locus_tag="AC16JC_05460"
     CDS             complement(520..1146)
                     /locus_tag="AC16JC_05460"
                     /inference="COORDINATES: protein motif:HMM:NF027589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HIR5164179.1"
                     /translation="MTTQYVAFIADLAASRALPARSRAALQTAVRSAARDFNVRYRRH
                     LAARFAVTLGDELQGLFAGAAPVWEVSHALRLRFPAVDWIVACGRGALSTPLHRGVTA
                     PELDGPCFHAARHALEHAKRLGLVFAFAGFDPALTACASYYSALYRSWTPRQRAVATA
                     QRATRSARLDELARLLRVGPSAISHLRRRMAWPLVAAGDSMFQELLAR"
     gene            complement(1232..2569)
                     /locus_tag="AC16JC_05465"
     CDS             complement(1232..2569)
                     /locus_tag="AC16JC_05465"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR5164180.1"
                     /translation="MAPRSRGLMVALAAIMISTGLGAQGRLRGVAYDSLLGRPLAGAS
                     VWLRDLNRSTLTDSSGRFVLDSMPPGRHVFLLAHPDLDSAGLGIVAAAVTVATGTEVA
                     LAVPSIGTVWRRLCGRDSIGADSAIVFGAVRDADSHALLSGVNVTIVWPHLTLAALRR
                     AGVETRADSATTDSTGTYRVCGVATGLNVRAQARAGSFTTGIVQLMADERPVARRDFF
                     LSRMPAGDSGQGGSTLTGRIRSSSGRPVAEAVVVLEGVDSAVSDANGRFTLGRLPGGT
                     QWLRARAVGFAPRETGVDLPSRGIDTLDLELHAITQLDTIRVVATARARRVLREVDER
                     RKLGLGYVLGPDEIRRRVTLRSVLQGFPSVTVQGTMNSFTVLMHSGSISRAYCVADLR
                     IDGIPSTWDLLSSYEPKDIAAVEVYPRASTVPAEYQNLFSGCGMVLVWTTYLR"
     gene            complement(2579..>3072)
                     /locus_tag="AC16JC_05470"
     CDS             complement(2579..>3072)
                     /locus_tag="AC16JC_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164181.1"
                     /translation="AAELAAGDADARAAESLARDGRYAEASARLAAAITSWTNAQLAA
                     QGRPRREGRIAARNDLEQLAAEFAAAFSAKSLPRLRVVYPRMTEAQSQEWGQLFLQAR
                     DITMVLNVGNVNRLGPAEVEATLSGSYEFTEMRGGSAESRTVSWQATLRLTPMGWRII
                     SLR"
BASE COUNT          499 a         1087 c         1085 g          401 t
ORIGIN      
        1 gggccagcag catcgaagtg agcgtgccga tcagcacata ttcggcgaag tcccggtcgt
       61 ccagagcgcg aaaccgggcg agcgccttgg cggcgaggat gaggcccagc gccccgaact
      121 gattgaccag cacgagtgtg agagcgagcg cgcgctccag cacgccgatc agcagcccgc
      181 gcgacaattc cgctgccgga acgttgatgc cgcccttggc gacgcgggaa gtggacgatt
      241 cgctcaccgg caccaggtcc agcactctcc ggaccaggtc ggcgccgccg gcgcaggcgt
      301 accagtatgc ggcgctcagc agaagcaggc ggccccagaa atacccctgg ctcgtcggat
      361 gcacgagacg gccgccgagc catcccagca ccaccgtccc cgccaacgcg ccggcctcgc
      421 agaggagggt acgcaccggg gtggtccagc cggcccggcg ccacggggcg aatccggcgg
      481 ccagggcgaa ggccaggagc agagcggcgg gaacggggtt catcgtgcga gcaactcctg
      541 gaacatgcta tcccccgcgg caacgagggg ccaggccatg cggcgccgca ggtgggagat
      601 ggcgctgggg ccgacgcgca gcagccgcgc cagctcgtcc agccgggcgc tgcgcgtggc
      661 gcgctgtgcg gttgcgacgg cgcgctgccg cggggtccag ctccggtaga gcgcggagta
      721 gtagctggcg caggcggtca gcgccggatc gaaccccgcg aacgcgaaca ccaggccgag
      781 gcgcttcgcg tgctccagcg cgtgccgggc ggcgtggaag caggggccgt cgagctcggg
      841 agcggtgacg ccgcggtgca ggggggtgct gagggccccg cgtccgcacg ccacgatcca
      901 gtccacggcg gggaaccgga gccgcagcgc gtggctgacc tcccagaccg gcgcggcgcc
      961 cgcgaacagc ccctgcagct catcgcccag ggtgacggcg aagcgggcgg cgaggtgacg
     1021 gcggtagcgg acattgaagt cgcgcgccgc ggagcgcacc gccgtctgca gcgccgcccg
     1081 ggacctggcg ggcagcgcgc gcgaggccgc gaggtcggcg atgaacgcta cgtactgagt
     1141 tgtcatatca cgtcaaaaat gcaggtcatg acgtaatacg tcaagttcag tgttcttgac
     1201 ggtatacgtc aatcctgcag aatggcgctg ctcagcgcag gtacgtcgtc cacaccagca
     1261 ccatgccgca tccgctgaag aggttctggt actcggcggg caccgtggaa gcccgcggat
     1321 acacctcgac cgccgcgatg tccttgggct cgtacgacga gagcagatcc cacgtcgatg
     1381 ggatgccgtc gatgcggagg tcggcgacgc agtaggcgcg cgatatgctc ccgctgtgca
     1441 tgaggacggt gaaggagttc atcgtcccct gcaccgtgac ggaggggaag ccctgcaaga
     1501 cggatcgcag cgtcacgcgc cgccggatct cgtccggtcc cagcacatag cccaggccga
     1561 gcttgcgccg ctcgtccact tcccgcagca cgcgccgcgc gcgtgcggtc gccaccaccc
     1621 tgatggtgtc gagctgggtg atcgcgtgga gctccagatc cagcgtatcg atgccgcggc
     1681 tcggcagatc gacgcccgtt tccctcggag cgaagcccac cgcgcgggcc cgcaaccact
     1741 gggtcccgcc cggcaggcga cccagcgtga agcggccatt tgcatcactg accgccgagt
     1801 cgacaccctc gagcacgacg actgcctccg ccaccggacg cccggagctg gagcgtatcc
     1861 ggccggtcag cgtgctgccg ccctgaccgg agtccccggc cggcatccgg ctgaggaaga
     1921 aatcccgccg cgccacgggg cgctcgtccg ccatcagctg aacgatgccc gtggtgaacg
     1981 aaccggcccg cgcctgggca cgcacgttca gcccggttgc caccccgcac acccggtaag
     2041 tcccggtgct gtcggtcgtc gcactgtcgg cccgcgtctc cacgcccgcg cggcgaaggg
     2101 cggccagcgt gaggtgcggc cacacgatcg tcacgttcac gcccgataac aatgcgtggg
     2161 aatcggcgtc ccggaccgcg ccgaacacga tggcgctgtc ggcgccgatg ctgtcgcggc
     2221 cacataaccg gcgccacacg gttccgatgg agggaacggc gagcgcgacc tcggtcccgg
     2281 tcgcgacggt caccgctgcg gcgacgatcc ccaatccggc gctgtcgagg tcgggatggg
     2341 cgagcaggaa cacgtggcga cccgggggca tgctgtccag gacgaagcgg ccggacgagt
     2401 cggtgagggt gctccggttg aggtcacgga gccagaccga tgcgccggcg agcggccggc
     2461 cgagcaggct gtcgtaggcg acgccgcgca gccggccctg ggctccgagc ccagtcgaga
     2521 tcatgatcgc ggcgagggcc accatgagcc cccgtgacct cggcgccata cgcctccgtc
     2581 agcgcaacga gatgatccgc cagcccatgg gagtgagccg cagggtcgcc tgccagctca
     2641 ccgtccggct ctctgcgctg ccgcctcgca tctcggtgaa ctcataggaa cccgacagcg
     2701 tcgcctcgac ctccgccggg ccgagacgat tcacgttgcc gacgttgagc accatcgtga
     2761 tgtcgcgcgc ctgcaggaac agctgccccc attcctggga ttgcgcttcg gtcatccgcg
     2821 ggtacacgac gcgcagccgc ggcaagctct tggcgctgaa cgcggcggcg aactcggccg
     2881 ccagctgctc cagatcgttc cgcgccgcga tgcgtccttc gcgccgcggc cgtccctgcg
     2941 ccgcgagctg ggcgttggtc caggacgtga tggcagctgc gagccgggcg ctcgcctcgg
     3001 cgtaccgccc atcccgtgcc agggattccg cggcacgggc atcggcatcc cccgccgcga
     3061 gctcggcggc gg
//
LOCUS       DBNGZW010000230         1388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_632557, whole genome shotgun sequence.
ACCESSION   DBNGZW010000230 DBNGZW010000000
VERSION     DBNGZW010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1388
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_632557"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1197)
                     /locus_tag="AC16JC_05475"
     CDS             complement(<1..1197)
                     /locus_tag="AC16JC_05475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5164182.1"
                     /translation="MRPAQGACRGTGAFAALGVTPILATLVLATGCKKETPQVVYRAV
                     PVEQRDIVVSAQAAGAIQPDTTVEVKSKASGEVLQLNAETGQLVKRGAPLVRIDPRNA
                     RNTLAQAQADLDVAQAKLTNATSQKRRADELYQSQSTTEQEHEQALLDYADASAQVVR
                     SRVAVDNARIQLEDTDVRAPITGTIIAKTVERGTVIASATSNVSGGTTLLQMADLNLV
                     QVRTLVDETDIGKVQPGQQATITVDAYPNRPFDGTVLKIEPQAQTEQNVTVFPVLVRI
                     QNREGLLRPGMNTEVEIHIGSRESVPAVPNAALRTQQDVGSAAQVLGLAADEVQRQLA
                     AQAAQSASSAGATAGTGVGGGRDTLRSPPDSLRGLRRDSLRRLGPDSADWRGQAPAGT
                     GTGRRTR"
     gene            complement(1194..>1388)
                     /locus_tag="AC16JC_05480"
     CDS             complement(1194..>1388)
                     /locus_tag="AC16JC_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015766472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding protein"
                     /protein_id="HIR5164183.1"
                     /translation="ADEPTGNLDSATSEEIMGLFEALYREGQTILLVTHEADIAAHAR
                     RQVHLKDGRVERDFATGRTA"
BASE COUNT          161 a          496 c          488 g          243 t
ORIGIN      
        1 ccgagttcgc cggcccgtgc ccgtcccggc gggcgcctgg ccccgccagt cggcggaatc
       61 cgggcccagg cggcgcaggg agtcgcgtcg caggccgcgg agcgaatcgg gtgggctccg
      121 gagcgtatcg cggcccccgc cgacgcccgt ccccgccgtg gccccggcgc tgctcgcgct
      181 ctgtgccgcc tgggcggcga gctgccgctg cacctcgtcc gcggccagcc ccagtacctg
      241 cgcggcggag ccgacgtcct gctgggtccg gagcgcggca ttgggaaccg cgggcacgct
      301 ctcccggctg ccaatgtgga tctcgacctc cgtgttcatg ccggggcgga gcagtccctc
      361 ccggttctgg atccggacca gcaccggaaa cacggtcaca ttctgctcgg tctgcgcctg
      421 cggctcgatc ttgaggacgg tgccgtcgaa ggggcggttg ggataggcgt cgacggtgat
      481 ggtggcctgc tggccgggct gcaccttgcc gatgtcggtc tcgtccacca gcgtccggac
      541 ctggacgagg ttgaggtctg ccatctggag cagcgtggtc ccgccgctga cgttcgaggt
      601 agccgaggcg ataacggtgc cgcgctcgac ggtcttcgcg atgatcgtac ccgtaatggg
      661 cgcacggaca tcggtgtcct cgagctggat tcgcgcgttg tccacggcca cgcgcgagcg
      721 cacgacctgg gcgctcgcat cggcgtagtc gagcagtgcc tgttcgtgtt cctgctccgt
      781 ggtcgactgg gactggtaca gctcgtcggc ccgccgcttc tggctggtcg cgttggtgag
      841 cttcgcctgg gccacgtcca ggtccgcctg ggcctgcgcc agggtattgc gcgcattgcg
      901 cgggtcgatg cgcacgagcg gcgcgccccg cttgaccagc tgaccggttt ccgcgttgag
      961 ctgaagcacc tcgcccgatg cctttgattt cacttcgacc gtagtatccg gctggatcgc
     1021 gcccgcggcc tgggccgaca cgacgatgtc gcgctgctcg acgggcacgg cgcgatagac
     1081 cacctggggc gtctccttct tgcacccggt ggcaagcacc agggtcgcca ggatcggggt
     1141 cactccgagc gcagcgaacg cgcccgtccc gcggcaggct ccctgcgccg gcctcacgcg
     1201 gtcctcccgg tggcgaagtc gcgctccact cgcccatcct tcaggtgcac ctggcgccgc
     1261 gcgtgggccg cgatgtcggc ctcgtgggtc acgagcagga tggtctgccc ctcccgatac
     1321 agcgcctcga acagccccat gatctcctcg ctcgtggcgc tgtcgaggtt gcccgtcggc
     1381 tcgtcggc
//
LOCUS       DBNGZW010000231         3789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_696787, whole genome shotgun sequence.
ACCESSION   DBNGZW010000231 DBNGZW010000000
VERSION     DBNGZW010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3789
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_696787"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1040)
                     /locus_tag="AC16JC_05485"
     CDS             complement(<1..1040)
                     /locus_tag="AC16JC_05485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR5164184.1"
                     /translation="MLEAPDFLKSLTIVLAVAAVTTIVFQRLRQPVVLGYLMAGLIVG
                     PHLPIPLVADPAVVRTLSELGVILLMFSLGLEFSLRKLAAVAPTAGLTALLQSSFMMW
                     LGFLLGRLFGWTGLESLFTGAIIAISSTTIIAKVFAEQGVTGKLRELVVGILIVQDLI
                     AVLLMAILTGVASGSGLAAGPLIALVARLGTFLFGLIAVGLLLVPRAMRAVIRLNRPE
                     TTVIASIGLCFSMALLAQAFGYSVALGAFIAGSLIAESGEEKAVERLVEPVRDVFAAV
                     FFVSVGMLIEPPLIAAHWSAVLVLTAVVVLGNVVGVSIGAFLTGNGMRTAVQAGMSLA
                     QIGEFSFIIAGLG"
     gene            complement(1096..1719)
                     /locus_tag="AC16JC_05490"
     CDS             complement(1096..1719)
                     /locus_tag="AC16JC_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011309960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HIR5164185.1"
                     /translation="MSTPAADFPATLQIDYPDRDLDRVTSFFRPFTVIPIAILLTLVS
                     GPSSRTEASRYALESGGIVFLPTVLMLLFRGKYPRWWFDWNVALTKFGLRVTAYLALL
                     RDEYPSTDAEQAVHLDVPYPDAAHLSRGLPLVKWLLAIPHYVVLGVLSLAAVGAVLVA
                     WFAVLFTGRYPRGLFAFVEGVLRWWVRVLAYAFLLTTDRYPPFRLAA"
     gene            complement(1743..1910)
                     /locus_tag="AC16JC_05495"
     CDS             complement(1743..1910)
                     /locus_tag="AC16JC_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008333370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164186.1"
                     /translation="MEKIRIEQHTFAGGLWFAGWLFTLGFLHLSFWKGFLALVVWPYY
                     LGVAVSTILQR"
     gene            2101..>3789
                     /locus_tag="AC16JC_05500"
     CDS             2101..>3789
                     /locus_tag="AC16JC_05500"
                     /inference="COORDINATES: protein motif:HMM:NF027232.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR5164187.1"
                     /translation="MTQRLWAAVTTLAALTTPLAAQTSTQLDSTLLAGFRWRNIGPAN
                     MGGRVSSVAGIPSPSRTFFLAAAAGGIWKTTNAGTTFRPVFDDQRCISMGELAIAPSD
                     TMQVWAGTGEEDSRNTISPGCGIFKSTDGGLTWKPMGLEKTGAIGRIVVHPTDPNIVY
                     VAALGQPWAANPERGLYKTTDGGQTWKLVKFISDRAGFIDLVMDPTNPEVLFASSWQR
                     VRGPYFLNSGGPGSALWKSTDGGTTWTEVKGGGFPETTKGRIGLAIAPSDHSIMYALV
                     EADTAPNPKPDKTKAAQTRPSGLYRSADGGATWTKMNSNDVRPFYYSQVRVDPKDPNR
                     VYWSSTPVNFSNDGGKTVGNATQGIHVDHHAMWIDPADANHIIVGDDGGVSQTWDKGG
                     NWEFLNVITIGQFYIVSYDMGFPYRVCGGLQDNGSWCGPSRHRQGTIDNSDWITVGGG
                     DGFYTAQDPTDPNTIYSESQGGNMGRLNLATGERTALQKPGWRPTYQLFEDSIVIERP
                     DTTKPATGPQRTRLADLRRRQLADSAEQDLRWNWNTPFFLSAHSPTTLYAGASKV"
BASE COUNT          740 a         1304 c         1243 g          502 t
ORIGIN      
        1 cccagcccgg cgatgatgaa ggagaactcg ccgatctggg cgaggctcat cccggcctgc
       61 accgcggtgc gcatgccgtt gccggtcaga aacgcgccga tcgagacgcc caccacgttg
      121 cccagcacga ccacggcggt cagcaccagc accgccgacc agtgggcggc gatcagcggc
      181 ggttcgatga gcatgccgac ggacacgaag aacaccgccg cgaagacgtc gcgcacgggc
      241 tccaccagcc gctccaccgc cttctcctcc cccgattcgg cgatgagcga gccggcgatg
      301 aaggcgccga gggccacgga gtacccgaag gcctgggcga ggagcgccat cgagaagcag
      361 aggccgatgc tggcgatcac cgtcgtttcg gggcggttca ggcggatgac ggcgcgcatc
      421 gcccgcggca ccagcagcag gcccacggcg atcagaccga acaggaacgt ccccagccgg
      481 gcgaccaggg cgatcagcgg cccggcggcc aggccgcttc ccgaggccac gcccgtgagt
      541 atcgccatca gcaggacggc gatcaggtcc tgcacgatga ggatgccgac gacgagctcg
      601 cgcagcttcc ccgtgacccc ctgctccgcg aagaccttcg cgatgatcgt ggtgctcgag
      661 atcgcgatga tcgcgccggt gaacaggctc tcgagtcccg tccagccgaa caggcgaccc
      721 agcaggaacc ccagccacat catgaaactg gactgcagca gggcggtcag ccccgcggtc
      781 ggcgccacgg cggcgagctt gcgcagactg aactcgagcc cgagcgagaa catcagcagg
      841 atcacgccca gctcggagag cgtccggacg acggccgggt ccgccaccag cgggatcggc
      901 aggtggggcc cgacgatcag cccggccatg agatagccga ggaccaccgg ctggcgcaga
      961 cgctggaaca cgatcgtcgt gaccgcggcg acggccagga cgatagtcag ggatttcagg
     1021 aaatcggggg cctcgagcat cggccctcaa acaatcacgc gcgctccgga aacgccagcc
     1081 ctgttacgcg ggcggtcagg ccgcgagacg gaacggcggg tagcgatcgg tggtgagcag
     1141 aaacgcgtac gccaggaccc gcacccacca ccgcagcact ccttccacga acgcgaagag
     1201 cccgcgggga tagcggcccg tgaacaggac cgcgaaccat gccacgagga cagcccccac
     1261 cgccgcgagg ctgaggactc ccagcacgac gtaatgcggg atggccagca gccatttcac
     1321 cagcggcaac ccacgactga ggtgcgcggc gtcagggtaa gggacatcca ggtggacggc
     1381 ctgctccgcg tcggtcgacg ggtactcgtc gcgcagcagc gccagatacg ccgtgacgcg
     1441 caggccgaac ttcgtcaacg cgacattcca gtcgaaccac cagcgcgggt acttgccgcg
     1501 gaacagcagc atcagcaccg tcggcagaaa cacgatgccg ccggattcga gcgcatagcg
     1561 cgaggcttcc gtgcgcgacg aaggacccga caccagcgtc aggaggatgg cgatggggat
     1621 gaccgtgaag ggacggaaga acgaggtgac ccggtccaag tcccggtccg ggtagtcgat
     1681 ctggagcgtc gcgggaaagt ccgccgccgg cgtgctcatg gagaggtcct cgatcgtacg
     1741 ggtcagcgtt gcaggatcgt gctcaccgcc acgccgaggt agtagggcca cacgacgagt
     1801 gccagaaacc ctttccagaa ggatagatgc agaaagccga gcgtgaacag ccatccggca
     1861 aaccacagcc ctcccgcgaa cgtatgctgc tcgatccgga tcttctccat ccccactcct
     1921 ggaatcctcg gggcggacgg tagtctgcag ggggcagtgg gaacggcgag tgaagcgcag
     1981 gtgacgcttt ctttgccggc cgcgagaggc ccggggagcg cgggctcaac ggccggggtc
     2041 tggcgtgcgt acccgaagtg ctgcagcatt ccccgactca cccttttgcc cgaggccgtc
     2101 atgacccagc gcttgtgggc ggctgtcacc acgcttgccg cactcaccac acccctcgct
     2161 gcccagacct ccactcagct cgattcgacg cttctcgccg gcttccgctg gcgcaacatc
     2221 ggccccgcca acatgggcgg tcgtgtgtcg agcgtcgccg ggatcccgag cccgtcgcgg
     2281 acgttctttc tcgcggccgc cgccggcggc atctggaaga ccacgaacgc cggcacgacg
     2341 ttccggcccg tgttcgatga tcagcgctgc atctcgatgg gggagctggc gatcgcgccg
     2401 agcgatacga tgcaggtctg ggcgggcacc ggcgaggaag attcgcgcaa caccatctcc
     2461 ccgggatgtg gcatcttcaa gtccaccgac ggcggcctca cctggaagcc gatgggtctg
     2521 gagaagaccg gggccatcgg gcgcatcgtg gttcatccga ccgatcccaa catcgtctat
     2581 gtcgccgccc tgggacagcc gtgggcggcg aacccggagc gcgggctcta caagaccacc
     2641 gacggcggcc agacctggaa gctcgtgaag ttcatcagcg accgggccgg cttcatcgat
     2701 ctcgtgatgg atcccaccaa ccccgaagtg ctgttcgcct cgagctggca gcgggtgcgc
     2761 gggccgtatt tcctgaacag cggcggcccc ggcagcgccc tgtggaaatc caccgacggc
     2821 gggacgacct ggaccgaggt gaaaggcggc ggcttccccg aaacgacgaa aggccgcatc
     2881 ggcctggcca tcgccccctc cgaccacagc atcatgtacg cgctggtcga agccgacacc
     2941 gcccccaatc cgaaacccga caagaccaag gccgcgcaga cgcgcccctc gggtctgtac
     3001 cgttcggccg acggcggagc gacctggacg aagatgaatt cgaacgacgt ccggcccttc
     3061 tactactcgc aggtgcgcgt ggatccgaag gaccccaacc gcgtctactg gtcctccaca
     3121 ccggtcaatt tctccaatga cggcggcaag acggtcggca acgcgacgca gggcattcac
     3181 gtggaccacc acgccatgtg gatcgatccc gccgacgcga accacatcat cgtcggagac
     3241 gacggcggcg tctcccagac ctgggacaag ggcggcaact gggagttcct gaacgtcatc
     3301 accatcggcc agttctacat cgtgagttac gacatgggat tcccctaccg cgtgtgcggt
     3361 gggctgcagg acaacggatc ctggtgcggg ccgagccggc accgccaggg cacgatcgac
     3421 aacagcgact ggatcaccgt cggcggcggt gacgggttct atacggcgca ggacccgacc
     3481 gatccgaaca ccatctattc ggaatcgcag ggcggcaaca tgggacggct caacctggcg
     3541 accggcgagc gcacggcgct ccagaagccc ggctggcggc cgacctatca gctgtttgag
     3601 gactcgatcg tcatcgagcg ccccgataca acgaagcccg cgaccggccc gcagcgcacc
     3661 cggctggccg acctgcggcg ccggcagctc gccgactcgg ccgagcagga cctgcgctgg
     3721 aactggaaca cgccgttctt cctgtcggcg cacagcccca cgacgctgta tgcgggcgcg
     3781 agcaaggtg
//
LOCUS       DBNGZW010000232         5753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1377843, whole genome shotgun sequence.
ACCESSION   DBNGZW010000232 DBNGZW010000000
VERSION     DBNGZW010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5753
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1377843"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="AC16JC_05505"
     CDS             complement(<1..690)
                     /locus_tag="AC16JC_05505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5164188.1"
                     /translation="MAKTFLLTDEFPPIMTGISRLMGEIARRYPKGELLVSTGQHHDA
                     PETDGQFTGAVIDRLPISTKALHGFAGLMFWSRRVGGLARAHKPRFAWADSIRPAGYP
                     AKWAYERVGVKYGVLVHGGDLLKELHAIHHTPFTRRSGKALLKSAAAVVANSHWTREQ
                     AQKVLREVGLDPLAERVKVVPLGTDPERFRPGIDARDVRDRYRLNGGRWAITVARLET
                     YKGIDTAMRAVA"
     gene            complement(690..1439)
                     /locus_tag="AC16JC_05510"
     CDS             complement(690..1439)
                     /locus_tag="AC16JC_05510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5164189.1"
                     /translation="MPAPLTVVIPTLNEGDQIAACVRHLAWADEVIVADGGSADGTVR
                     AARAAGATVLEVPGVTIAAQRNAAIGRARNAWVFALDADERIGPDLEQELSGLAAGAA
                     RHEAYAVRRNNVYLGRRMRYAGWGDGWAVRFFKRERRFVEKRVHEGLEPVADVARLNG
                     ALEHTPYRDLSHHIRKLVQYAEWGALDLRDAGRRARATDLLVRPGWQAFRTYVLQLGV
                     LEGWRGVVLCGLAGVSVFLKYAHLWDLQRRG"
     gene            complement(1483..4506)
                     /locus_tag="AC16JC_05515"
     CDS             complement(1483..4506)
                     /locus_tag="AC16JC_05515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164190.1"
                     /translation="MAARAASLAAVVLAVASISQAQDTLPPSQGGYVHSLGLPEIYKP
                     YIGVGIGLSNADGHHLASQARLGVFRDIGNPVTELLGWDLEGYAGLRDVRIDGGARGL
                     LLTNLFRLGAGVDYSAHDGADFVLAYVASGRRGGVIGRGSELRFEWLPTRHSSVNLAV
                     NVPLGQPHRGRTRPDRVYVELHPDRPRPLPLALYPALGPVLDSLRNMAFWIDRLTVPK
                     LGVCQGDAAEAAAAVASPLKAHLADHTVTDDIRAYHRLLTRAFSIVLLGPTVSNGEVL
                     EHVVADTAKAILLNRVLFPYNRLIGQPKREDTTLEFGGNARGVFARWLVGKSLVPDDR
                     TDGAIYVFQRLLDIVEEVRADNRQAWGDSRLVWLPLQLALTPEQYDGTDKLDTLISHA
                     VGHPMTHGNRVWYVQNSRFQERLVQSISEASEYHIVWIHDFRGLNDSGRPDRLSLLVV
                     TDAYLKALTERVSRYDSTGILPAYMIFLDQHYFEKNRSRALLNVLQDPLGRTLDLPAG
                     DSLGDRLRAAQDRLRRTVDASRLLRVERAQYGELWLHRLVKVHINITNPADPSFRSRQ
                     ILPVIGMPDDAIRDHRKVVLYDVSEEDPYRGMAMYAGAGVGEHYAGVAWEDRAMMVQG
                     PAALALRDAARRLLEAQGIVGGEMPHVLRPHRWASDYWTWVRIEIDSLDRIGKVASRA
                     IELHNGTGFDVKEVSVGEATLFNLLSPGGVVKVPDSLWLNELLASLLLGDALRGVRVL
                     VIAPSLASAPAAAAQNMAAVHELLSRLVALHRVLGPEAARAGGLLRVGLYNPSIDVDD
                     LAGRVDALLRSLRADSGLRNLYQFDTASYAALEQVLGVLKGLRTPDTNGSRLQVHPKL
                     HFKGFLYVSREPWKYLISGPPMADGMRVYLEELIRQRREGVREGEEDMAQVLQQVGAK
                     VINPIIGRMPEVEWKHWAFLLAVGSANQDYRSMTMDGEATILVTNWTALYALSDFILL
                     TGLVDWVDDQAALDRGLPPPSGFKRWLACWIKMAL"
     gene            complement(4537..5283)
                     /locus_tag="AC16JC_05520"
     CDS             complement(4537..5283)
                     /locus_tag="AC16JC_05520"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR5164191.1"
                     /translation="MRPALSVHDATPDSIRIVTFNIKFAQHVDRAAELLQSAPALHPA
                     DIVTVQEMDEPGVRALAAALGMSYVYYPATVHPQTGRDFGNAVLSRWPITGDRKVILP
                     HLGRWERTERAAVGATILVGAIPIRVYSVHLGTPLEIGPGGKRDQVRAVLRDAVAYSH
                     VVIAGDMNGQSIGREFVAHGYRWPTEHEGPTSSFFSFDHIFLLGLGDRAHTGVTRDDL
                     QASDHRPVWATIGLGPGFPRSFTQSSGTGP"
     gene            complement(5414..>5753)
                     /locus_tag="AC16JC_05525"
     CDS             complement(5414..>5753)
                     /locus_tag="AC16JC_05525"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024914.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5164192.1"
                     /translation="VAALPSRIEGLSQSLLEAMALGLPVVASDAGGNADLIAAGRTGL
                     LVPPLEPAAWSTALDRLLGAPEPAAGMAAAGRALVRREFTIERTAERTETVYREALHR
                     RLHARPGASA"
BASE COUNT          958 a         1991 c         1909 g          895 t
ORIGIN      
        1 ggccacggcg cgcatcgccg tgtcgatccc cttgtaggtc tcgagccgtg ccacggtgat
       61 cgcccagcgc ccgccgttca agcggtaacg gtcccgaacg tcgcgggcgt cgatcccggg
      121 ccggaaccgc tccggatcgg tccccagggg cacgaccttc acccgctcgg ccaggggatc
      181 gagtcccacc tcgcgcagca ccttctgcgc ctgctcccgc gtccaatgac tgttggcgac
      241 caccgcggcc gccgatttca ggagcgcctt ccccgaacgt cgcgtgaacg gcgtgtgatg
      301 gatggcgtgc agctccttca ggaggtcgcc gccgtgtacc agcacgccgt atttcacgcc
      361 gacgcgctcg tacgcccact tggcgggata gccggccggg cggatcgagt cggcccaggc
      421 gaaccgcggc ttgtgggccc gcgcgaggcc gccgacccgg cgcgaccaga acatgaggcc
      481 agcgaagccg tgcagcgcct tggtggagat cggcaggcgg tcgatcaccg cgccggtgaa
      541 ctgtccgtcg gtctcgggcg catcgtggtg ctggccggtg gagacaagca gctccccttt
      601 cgggtagcgg cgggcgatct cgcccatcag gcgggagatg ccggtcatga tcggcggaaa
      661 ctcatcggtc agcaggaagg ttttggccat cacccgcgtc gttgaaggtc ccagaggtgg
      721 gcgtatttga gaaacacgct caccccggcg agcccacaca gcaccactcc ccgccacccc
      781 tcgagcaccc cgagctgcag cacgtaggtc cgaaacgcct gccagccggg tcgcaccagc
      841 aggtcggtcg cgcgcgcccg gcggccggcg tcgcggaggt cgagggcgcc ccactccgcg
      901 tattgcacca gcttgcggat gtggtgcgac aggtcccggt agggcgtgtg ctcgagcgcc
      961 ccgttcagcc gggccacgtc ggccaccggc tcgaggcctt catggacccg cttctccacg
     1021 aaccggcgct cgcgcttgaa gaagcggacc gcccatccat ccccccatcc cgcgtaacgc
     1081 atgcggcgcc ccaggtacac gttgttgcgc cgcaccgcgt acgcctcgtg ccgggccgcg
     1141 cccgccgcga gacccgacaa ctcctgctcg aggtcgggcc cgatgcgctc atcggcatcg
     1201 agcgcgaaca cccaggcgtt ccgtgcccgg ccgatagcgg cgttgcgctg cgccgcgatc
     1261 gtgacgcccg gcacctcgag caccgttgcc ccggccgctc tggcggcgcg aaccgtccca
     1321 tccgcggagc cgccgtcggc cacgatcact tcgtccgccc acgcgagatg ccgcacgcag
     1381 gcggcgatct ggtccccttc attgagggtc gggatgacga ccgtgagcgg ggccggcatg
     1441 tcagccagaa cactacacca gccggtccct tccctgcaat cgctacaggg ccatcttgat
     1501 ccagcaggcg agccaccgct tgaaaccgct cggcggggga aggccgcggt cgagggcggc
     1561 ttggtcgtcc acccagtcta ccagtcccgt cagcaggatg aaatccgaga gtgcatacaa
     1621 ggcggtccag ttagtcacaa ggatcgtcgc ttcgccgtcc atcgtcatgc tgcggtagtc
     1681 ctggttcgcc gagcccaccg cgaggaggaa cgcccagtgc ttccattcca cctccggcat
     1741 ccgaccgatg atcgggttga tgaccttcgc ccccacctgt tgcagcacct gagccatgtc
     1801 ctcttcgccc tcacggaccc cttcccgccg ctgccggatc aattcctcca ggtacacgcg
     1861 catcccgtcc gccatcggcg gcccgctgat gaggtatttc cagggttctc tggagacgta
     1921 gagaaacccc ttgaagtgca gcttgggatg aacctggagt cgcgagccgt tcgtgtccgg
     1981 ggttctgagg cccttcaaca ccccaagcac ctgctcgagg gcggcgtagg acgccgtatc
     2041 gaattggtag aggttccgca gtcccgaatc cgcccgaagg ctgcggagta gcgcgtcgac
     2101 tcggccggcg agatcgtcca cgtcgatgga cgggttgtac aggccgaccc gcaggagccc
     2161 gccggccctg gccgcctcgg gacccagcac gcggtgcaac gcgacgaggc gggacaggag
     2221 ctcgtgaacc gccgccatgt tctgcgcggc agcggcggga gcggaagcga gcgagggagc
     2281 gatgaccaac acgcgtacgc cgcgcagcgc atcacccagg agcagggatg cgagcagctc
     2341 attgagccag agtgagtcgg ggaccttcac gacaccgccc ggtgagagca ggttgaagag
     2401 cgtcgcctct cccacggaca cctctttcac atcaaacccg gtcccgttgt gcagttcgat
     2461 cgcgcgactc gcgaccttcc cgattcgatc caggctgtcg atctcgatcc gcacccacgt
     2521 ccaataatcc gacgcccagc gatgcggccg cagcacgtgc ggcatctcgc cgcccacgat
     2581 cccctgggcc tcgagcagtc gtcgcgccgc gtcacgcagt gccagggcag ccggaccctg
     2641 caccatcatg gcgcggtctt cccacgccac acccgcgtag tgctccccaa ctcctgcgcc
     2701 ggcgtacatc gccatgccgc gataggggtc ctcctcgctc acatcgtaca ggacgacctt
     2761 cctgtgatcg cggatcgcat cgtcgggcat accgatcacg ggcaggatct ggcgactgcg
     2821 gaacgagggg tcggccggat tggtaatgtt gatgtgcacc ttgacgagac ggtgcagcca
     2881 cagctcgccg tactgggccc gttcgacgcg cagcagccgc gatgcgtcca ccgtacgccg
     2941 cagtcggtcc tgagccgcac gcagcctgtc gcccaaggaa tccccggccg ggagatcgag
     3001 ggtgcgtccg agcgggtcct gcaggacgtt cagcagcgct cggctccggt tcttctcgaa
     3061 atagtgctga tccagaaaga tcatgtatgc cggcaagatc ccggtcgaat cgtagcgcga
     3121 tacgcgctcg gtcagcgcct tgagataggc gtccgtcacg acgagcagcg acagacgatc
     3181 cggccggccg gagtcgttca gccctctgaa gtcgtgaatc cacacgatgt ggtattccga
     3241 tgcctcgctg atggactgga ccagtcgttc ctgaaagcgc gagttttgga cgtaccacac
     3301 gcgattgccg tgggtcatcg gatggcccac cgcgtggctg atcagggtat cgagcttgtc
     3361 cgttccatcg tactgctccg gcgtgagggc gagctgcaac gggagccaca cgaggcgcga
     3421 gtccccccag gcctgccgat tatcggcgcg tacctcctcc acgatgtcga gcaaacgttg
     3481 gaagacgtat atcgccccgt cagtccgatc gtccggaacg agcgatttcc ccaccagcca
     3541 gcgtgcgaac accccccgtg cgtttccgcc gaactcgagg gtcgtgtcct cgcgcttcgg
     3601 ttgaccgatc aggcgattgt acgggaagag cacgcgattc agcaggatgg ctttggccgt
     3661 gtccgccacg acgtgctcca ggacttcgcc attgctcact gtcggaccga gcaggacgat
     3721 cgagaaagcg cgggtcagca gccggtggta ggcgcggatg tcgtccgtga cggtatggtc
     3781 cgccaggtgt gccttcaggg ggctggccac cgctgcagct gcctcggcgg cgtccccttg
     3841 gcatacgccg agcttcggga ccgtcagccg gtcgatccag aacgccatgt tccgaagcga
     3901 gtcaagaacg ggcccgagcg ccgggtacag cgccagaggg agcggacgcg ggcggtcggg
     3961 atggagctcg acatatacgc gatccggacg tgtccggccg cggtgcggct ggccgagcgg
     4021 aacgttgacg gccaagttca cggaactgtg gcgcgtcggc agccactcga agcgcagctc
     4081 gctgccgcgt ccgataacgc cgccgcgccg cccggaggcc acgtaggcca gcacaaagtc
     4141 tgcgccgtcg tgggcgctgt aatcgacgcc cgctcccagc cggaacagat ttgtcaggag
     4201 caggccgcgg gcgcctccgt cgatgcggac gtcgcgcaac ccggcatagc cctccagatc
     4261 ccaccccagt agctccgtga ccggattgcc gatgtcacgg aacacaccga gtcgcgcctg
     4321 tgaggcgaga tggtgaccgt cggcattcga gagaccgatc ccgacgccga tgtatggctt
     4381 gtagatctcg gggaggccga gggaatggac gtagccgccc tggctgggcg gcagggtgtc
     4441 ctgcgcctgg ctgattgaag caacagcgag cactactgca gcgagggatg ctgcgcgggc
     4501 cgccattcct cctccacccc gacttccagg aagaggctaa ggcccagtcc ctgaagattg
     4561 ggtgaaggaa cgcggaaagc cggggcccag cccgatggtc gcccaaacgg ggcgatggtc
     4621 gctggcctgg agatcgtcgc gagtcacccc ggtgtgggcg cggtcgccca atccgagcag
     4681 gaagatgtga tcgaaactga agaagctcga ggtgggcccc tcgtgctcgg tgggccaccg
     4741 gtacccgtgc gccacgaatt cgcggccgat gctctgcccg ttcatgtcgc cggcgatgac
     4801 cacgtgcgag taggccacgg cgtcgcgcag tacggcgcgc acctggtcgc gcttgccccc
     4861 cggtccgatc tcgagcggcg tgccgaggtg gacgctgtag acccggatcg ggatcgcccc
     4921 tacgaggatc gtcgcgccga ccgccgcgcg ctcggtccgc tcccagcggc cgaggtgcgg
     4981 caggatcact ttcctgtcgc cggtgatcgg ccagcgcgac agcaccgcgt taccgaaatc
     5041 cctgccggtc tgggggtgta cggtggcggg atagtacacg taggacatgc cgagcgcagc
     5101 ggccagggcc cgcacgcccg gctcatccat ctcctgcacc gtgacgatgt ccgcggggtg
     5161 gagcgccggc gcagactgga gcagctcggc ggcgcggtcg acgtgttggg cgaacttgat
     5221 gttgaacgtg acgatgcgaa tcgaatccgg cgtagcgtca tgaacgctca gcgccgggcg
     5281 caccggctcg acgcccccga cgtagcgtgg gccgtctggg ccggagtagt tgagcgccgg
     5341 gcgacagcca gtgccgccga cgagcgctgc gaaggcaaga ggtgggatcc gtcgaagcat
     5401 ccgcacgcgg aatttacgcg gatgccccag gccgagcatg aaggcggcgg tgcagcgcct
     5461 cccggtacac cgtctccgtc cgctccgccg tccgctcgat cgtgaactcc cgccgcacca
     5521 gggcgcgtcc cgccgccgcc atgcccgctg ccggctcggg ggcaccgagc aggcgatcga
     5581 gcgcagtcga ccacgcggcc ggctcgagcg gtggcacgag caagccggtg cgcccggcgg
     5641 cgatcaggtc ggcgttgccg cccgcatcgg acgccacgac gggcagcccc aacgccatgg
     5701 cctcgagcag gctctgcgac agtccttcga tgcgcgacgg cagcgccgct acg
//
LOCUS       DBNGZW010000233         2274 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1246442, whole genome shotgun sequence.
ACCESSION   DBNGZW010000233 DBNGZW010000000
VERSION     DBNGZW010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1246442"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="AC16JC_05530"
     CDS             complement(<1..616)
                     /locus_tag="AC16JC_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164193.1"
                     /translation="MLAAAALASAVAAASAEAQRPAGASRPVVGVEISRENIFDSTET
                     GRWYARAINALHMRTRAFVVRRELLIVPGMPFDSALAAETSRNLRSLGFFRVARVDSV
                     ASDSGLLLRVTTKDRWTTKGNAGARLTGGQTLLNLALAESNLLGTGTSLGFRFRNEPD
                     RTSLRLATVVPRALAGMVDVRAQYDRLSDGDVTAAALISPFRALG"
     gene            complement(691..2178)
                     /locus_tag="AC16JC_05535"
     CDS             complement(691..2178)
                     /locus_tag="AC16JC_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR5164194.1"
                     /translation="MQSYSSHDALSLAELVRTKQVTPRELVTASIERIESLNPRLNAV
                     VHRMFEAALKQADGPLPDGPFRGVPFMLKDLLSWYEGEPLTMGSRLFKGWIPPQDTEI
                     VTRYRRTGVIVVGKTNTPEFGLTPFTESEFLGPAHNPWDPTRTTGGSSGGSAAAVASG
                     MVPWAGGGDGGGSIRIPASCCGVFGLKPTRARTPAGPVAGEQWQGAAIEHCITRSVRD
                     SAAMLDAIAGPDTGAPYWAPPPARSYLEEVGVAPGRLRIAFSSAPLLGHKVHGECQAA
                     LSDAVRLLESLGHELVEAAPTVDRDAFNRAFLTVVCGEVHADLTEARQLAGRVATPDN
                     VEYTTWALNLLGGQLTAGDFAFAERQLRTTARGVGEFFTRFDAFLTPTVAMPPFIIGA
                     LQPPAHEQALLRVLGRLRAGSVLRMMGMLEQAAGQVFDFIPYAPLFNVTGQPAMSLPL
                     YWTPENLPVGVQVVGRFGDEATLFRLAGQVEAARPWKDRHPPIFA"
BASE COUNT          398 a          777 c          762 g          337 t
ORIGIN      
        1 caccgagcgc gcggaaaggg ctgatgagtg ccgccgcggt cacgtcccca tccgagaggc
       61 ggtcgtactg agcgcgcacg tccaccatgc cggccagcgc gcgcgggacc accgtcgcca
      121 gccggagcga cgtgcggtcc ggctcgttgc gaaaccggaa gcctaacgat gtcccggtcc
      181 ccagcagatt cgactcggcg agcgcgaggt tcagcagcgt ctgtccgccg gtcaggcgcg
      241 cgccagcatt ccctttggtc gtccaccggt ccttggtggt gacgcgcaga agtagtccgc
      301 tgtcgctggc gaccgaatcg acacgtgcca cccggaagaa accgagactt cgaaggttgc
      361 gcgaagtttc ggcggccagc gcggagtcga acggcatgcc cgggacaatg agcagttcgc
      421 ggcgcaccac gaacgcgcga gtccgcatgt gcagcgcatt gatggcgcgt gcgtaccagc
      481 gcccggtctc ggtcgagtcg aaaatgtttt cccggctgat ctcgacaccg accacgggac
      541 gcgaggcgcc ggccggacgc tgtgcctcgg cggatgccgc cgccacggcc gaggcgagcg
      601 ccgcggccgc gagcacggca cgccgtcgac aggtcttcag ctgcaacgtc actcgggaag
      661 gcgtgggatg acgaccgtgc gcgacgcccg tcaggcgaag atgggagggt gacggtcctt
      721 ccacggacgg gccgcctcca cctgacctgc cagacggaag agcgtcgctt cgtcgccgaa
      781 gcggcctacc acctgcacgc ctaccggcag gttctccggg gtccagtaca gcggcaggga
      841 catcgccggc tgcccggtga cgttgaagag cggcgcgtac ggaatgaagt cgaacacctg
      901 cccggcggcc tgttcgagca tccccatcat tcggagtacg ctacccgccc gcaaccggcc
      961 cagcacccgc aggagagcct gttcgtgcgc cggcggctgc aacgctccga tgatgaacgg
     1021 cggcatggct acggtcggcg tgagaaaggc gtcgaatctg gtaaagaact cgcccacgcc
     1081 gcgcgcggtg gtgcgcagct gccgctcggc gaacgcgaaa tcccccgccg taagctgacc
     1141 gccgaggaga ttgagcgccc acgtcgtgta ctcgacgttg tcgggcgtgg ccactcggcc
     1201 ggcaagctgc ctcgcctccg tcaggtcggc gtgcacttcg ccgcagacga cggtcaggaa
     1261 ggcgcgattg aacgcatccc gatccaccgt cggtgcggcc tcgacgagct catgtcccag
     1321 cgactcgagc agccgcacgg catcggagag cgcggcctga cactcgccgt gcaccttgtg
     1381 cccgagcaat ggagcgctgc tgaaggcgat acgcagtcga ccaggtgcaa cacccacctc
     1441 ctcgagatac gaccgcgcag gcggcggcgc ccagtacggc gcccccgtat ccggtccggc
     1501 gatggcgtcg agcatggcag cgctgtcccg caccgagcgc gtgatgcagt gttcgatggc
     1561 ggctccctgc cactgctctc cggccacggg cccagccggc gtgcgcgcgc gcgtcggttt
     1621 gagaccgaag acgccgcagc acgacgccgg aatgcgaatc gacccgccgc cgtcccctcc
     1681 gccggcccac ggcaccatcc cgctcgccac cgccgctgcc gatccgcctg acgatccacc
     1741 agtggtccga gttggatccc aagggttgtg ggccgggccg aggaactccg attcggtgaa
     1801 tggagtgagt ccgaattcgg gtgtattcgt cttcccgacc acaatcacgc ccgtacgacg
     1861 gtaacgtgtg acgatctccg tgtcctgcgg tggaatccac cccttgaaca accggctgcc
     1921 catcgtgagg ggctcaccct cgtaccagga cagcaggtcc ttgagcatga agggaacgcc
     1981 gcgaaacggt ccatcgggga gcggaccgtc agcctgtttc agggccgcct cgaacatgcg
     2041 atgaacaacg gcgttgagcc tggggttcag tgactcgatg cgctcaatgg acgccgtcac
     2101 gagctcgcgg ggcgtgacct gttttgtccg caccagctca gcgagggaga gggcgtcgtg
     2161 agaggagtag gattgcaatg gcagggaagt aagtagtccc ccaagctgac agcgcgacgg
     2221 cccgacgtcc acctgttgcg ctggcgcgcc gcgcggccat cgtccagact atcg
//
LOCUS       DBNGZW010000234         6909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_906922, whole genome shotgun sequence.
ACCESSION   DBNGZW010000234 DBNGZW010000000
VERSION     DBNGZW010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6909
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_906922"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="AC16JC_05540"
     CDS             complement(<1..853)
                     /locus_tag="AC16JC_05540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5164195.1"
                     /translation="MTAAFDPAGSLARELARLVGPRHVRVAASERLTYASDGLPTHRR
                     TPGLVVLPGTRDEVVAVIRTLARLEVPFVPRGAGTGLSGGALAAPDAVLLVLTRLNRI
                     LEVDARNRRAVVEPGVVNVRLSAAAAPHGLHYAPDPSSQTACTIGGNVAENAGGPHCL
                     KHGTTTQHILALEVVLADGSVVCFGSPGGEPWGPDLVDLFVGSEGNFGVATRINVRLT
                     PAPRAVRTLLADFPSLRNAGAAVSAVIASGVVPAALEMMDRSCVNAVEDSVYAAGYPR
                     DAAAVLLA"
     gene            complement(934..1827)
                     /locus_tag="AC16JC_05545"
     CDS             complement(934..1827)
                     /locus_tag="AC16JC_05545"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5164196.1"
                     /translation="MARASTGKSKSFFKAAPSGAFDQYLQDIQKLPLIKDPAEERRLA
                     RRVQRGDEKAAERLVTANLRFVISYVKKYQGHGLDLSELVAIGNEGLLKAVKKFDPDQ
                     GVKFISYAVWWVRQAVLKALAEQTRSVRIPLNQNSQLIRMSRTETYLSQELGREPTDD
                     EIALALEDTVENVRTARRMTAAELSLDAPVDRTDKDAATLGERFAGIEGGEIEERTDG
                     RLMREFIDRIFQKYLTPRERKILYLYYGLEEGSEAMTLEKIGALMGVTRERIRQIRER
                     AFEKLRGSPDGRALKGFWRAA"
     gene            complement(2097..3212)
                     /gene="aroB"
                     /locus_tag="AC16JC_05550"
     CDS             complement(2097..3212)
                     /gene="aroB"
                     /locus_tag="AC16JC_05550"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015738684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR5164197.1"
                     /translation="MSIRVPLRETRDASYDILIDRGILPRLPALLTEHCPAAAYAVIG
                     DSHVAKVYGERVTTSLRAAGMVAELLTFPAGEWNKTRDTWTALCDRMLAAHFGRDGAV
                     LAVGGGVTGDVAGFVAATYYRGVPWVQVPTSLLAMIDSSIGGKTGVDTPQGKNLLGAF
                     HQPRLVVADLDVLATLALPQLAAGMAEAIKHAAIADADYFALLERSPVPVTAEAATLE
                     RVVARSVEIKAEIVAADEREKGRRASLNFGHTVGHALEATSGYALLHGEAVAIGMVAE
                     ARLAEAIGVAEQGTAARIARALERQGLPVTIPEGASAESLLDAMRHDKKVRGGSLRFA
                     LPSRVGTLGPNAVGEWTVAPSDAQLRRSLEAGLVAQG"
     gene            complement(3209..3754)
                     /locus_tag="AC16JC_05555"
     CDS             complement(3209..3754)
                     /locus_tag="AC16JC_05555"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR5164198.1"
                     /translation="MKIYTKTGDAGETGLFGGERVPKDHRRVVAYGEVDELSAALGLA
                     RATDPPGFEDALLQAIQRSLFTIGAELATPDPARLAKALGGPPLGEGEVTALEQAIDR
                     LEAGLPPLKAFILPGGAPKGAAFHVARTVCRRAERAVVGLGRETPVSPAIRHYLNRLS
                     DLLFVLARAANARAGAGDLPW"
     gene            3733..3882
                     /locus_tag="AC16JC_05560"
                     /pseudo
     CDS             3733..3882
                     /locus_tag="AC16JC_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748839.1"
                     /note="catalyzes the oxidation of ferredoxin with NADP;
                     incomplete; partial in the middle of a contig; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
     gene            complement(3975..5078)
                     /locus_tag="AC16JC_05565"
     CDS             complement(3975..5078)
                     /locus_tag="AC16JC_05565"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:NF013939.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HIR5164199.1"
                     /translation="MAASLEELRREIASVDAALVELVASRLALAAEVGEAKRAAGLPV
                     RSFSTEAEVLERFQRLGAAAGLDERLAERIAHLLIGAAVRVQETSPGTGAARGLRILL
                     VGGAGKIGRWFAEFFAGQGHRVSTLDPAGPVAGFPAVSDLATGVHAAEAIIITTPLTV
                     GREVLDRVFGLAPDGLVADAFSLKSHVLDLLEGATGRGLRVASLHPLFGPGVRTLSER
                     IMAVCDCGNATAADEAAALFTDTALTITRLPVARHDEYMQYVLGLSHLVAILFFTTLA
                     RSGRSYEDLARVASTTFLKEARTAAEVSRENPGLYFEIQRLNVHSAELFALVRGCLGE
                     IEIAALADDPARFEQVMRDGRSFFPESLPAELG"
     gene            complement(5078..6595)
                     /gene="dacB"
                     /locus_tag="AC16JC_05570"
     CDS             complement(5078..6595)
                     /gene="dacB"
                     /locus_tag="AC16JC_05570"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine endopeptidase"
                     /protein_id="HIR5164200.1"
                     /translation="MRKPILVSLTISCALGAPLTAVAQTAARPGRGSLARRLDALLDR
                     RPFDRVTWGVSVVDERGRTLYERNADRLFVPASNTKLVVTSAATVLLPADYRVRTSVY
                     INGVLQDGILDGDLILYGRGDPTFSERCYGVDTLAPGACDSAFTAINAIADSIRAHGV
                     TRITGHIVGDGSYFEPQLVRPAWNVFDLNWWYAAPVSGLGFHDNSVDFLITPSDTVGR
                     PPTITWSPELSLFTFENRARTGPAGGGSSIGDNFFRAPGTMDIWAEGTAARGRKPWTE
                     SFALPDPNRYAARALAFALERAGITVEGGATSTTDSLAYRAARCCAPALVEYSGRPLA
                     DILFPILNSSQNWFAEMLLKILGREQGGTGSWDAGLEVERRFLVDSVGIDSSAFSLED
                     GSGLSAGNLVAPRAFTRLLSYMAAHPKRDPFLAAMPRAGQPGSLLRRFVGTPLEGAVV
                     AKTGSIDRVNTLSGYIDRPGGGRLVFSIEANAHAVSGRAMLAQIDSLVVAIGRAK"
     gene            complement(6561..>6909)
                     /locus_tag="AC16JC_05575"
     CDS             complement(6561..>6909)
                     /locus_tag="AC16JC_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5164201.1"
                     /translation="TRGAMREALASDFIRTARAKGLPELAVVTRHALRNALIPVITVL
                     ALDTGNYLTGSLLTESIFAWPGLGRYVLTAIEKRDLPAVQGAILFMSLVFVFVNLVAD
                     LLYAKADPRIAHD"
BASE COUNT         1081 a         2414 c         2393 g         1021 t
ORIGIN      
        1 cggccagcag cacggccgcc gcgtcgcgcg gatagccggc ggcgtagacc gagtcctcca
       61 ccgcgttgac gcaggaccga tccatcatct cgagggcggc gggcaccacg ccgctggcga
      121 tgacggcgga gaccgctgcg ccggcgttgc gcagcgacgg gaagtcggca agcagcgtgc
      181 ggaccgcgcg gggagcgggg gtgagccgca cgttgatccg ggtggccacg ccgaagttgc
      241 cctccgagcc gacgaacagg tccaccagat cgggacccca gggttcgccg ccgggactgc
      301 cgaagcacac gaccgagccg tcggcgagga cgacttccag cgccaggatg tgttgggtcg
      361 tggtgccgtg tttcaggcag tgcggacccc cggcgttctc cgccacgttc ccgccgatgg
      421 tgcaagcggt ctggctcgat ggatccgggg cgtaatgcag cccgtgcggc gccgcggcgg
      481 ccgagagccg gacgttgacc acacccggct ccaccaccgc gcgccggttg cgcgcgtcga
      541 cctcgagaat gcggttgaga cgggtgagca ccagcaggac cgcgtccggc gcggcgagcg
      601 ctccgcccga gagtcccgtg cccgccccgc ggggcacgaa cggcacctcg agccgggcga
      661 gcgtgcggat caccgcgacg acctcgtcgc gggtgccggg gaggacgacg agtccgggcg
      721 tgcgccggtg cgtgggaaga ccgtccgagg cgtaggtgag acgttcactc gccgcgacgc
      781 gcacgtggcg tggcccgacg agccgtgcca gctcccgggc cagggacccg gcggggtcga
      841 aggcggccgt catgagtcga atactatacg gtcaaagaaa gaggggccgg gaatgagttc
      901 ccgacccccc ggattcgctc accgtcccga tgatcaggcc gcgcgccaga accccttgag
      961 cgccctgccg tcgggcgacc cgcgcagctt ttcgaaggcc cgctcccgga tctggcggat
     1021 acgctcccgg gtgacgccca tgagcgcgcc gatcttctcc agcgtcatgg cttcgctgcc
     1081 ttcttcgaga ccgtagtaga ggtagaggat cttgcgttcg cggggcgtga ggtacttctg
     1141 gaagatgcgg tcgatgaact cgcgcatcag ccggccgtcg gttcgctcct cgatctcgcc
     1201 gccctcgatg cccgcgaacc gctcgcccag ggtggccgcg tccttgtcgg tgcggtccac
     1261 cggcgcatcg agcgacagct cggccgccgt catgcgacgc gcggtgcgca cgttctccac
     1321 cgtgtcctcg agcgccaggg cgatctcgtc gtcggtcggc tcgcgcccca gctcctggga
     1381 caggtaggtc tcggtgcggc tcatgcggat cagctgcgaa ttctggttga gcggaatgcg
     1441 gacgctgcgc gtctgctcgg cgagggcctt gaggacggcc tggcggaccc accacacggc
     1501 gtaggaaatg aacttcacgc cctgatcggg gtcgaacttc ttcacggcct tgagcaggcc
     1561 ttcgttgccg atcgccacga gctcggagag atcgagtccg tgcccctggt acttcttcac
     1621 gtaggagatc acgaaccgca gattcgccgt gaccagtcgc tccgccgcct tctcgtcgcc
     1681 acgctgcacg cgacgggcca accgccgttc ctcggcgggg tccttgatca gggggagctt
     1741 ctgaatgtcc tgcaggtact ggtcgaaggc accggacggt gcggctttga agaaggactt
     1801 ggactttcca gtcgacgcac gggccatgcg ctggactcca tagcagggca tacgcgttct
     1861 agccggagac ggcgcgactg accgcctaga gtgacctgaa agcgggcaca atataggaac
     1921 gcccgtcaac acgtgcaaat ctactaggtt ttccacatca caaattgtta ttacgcgcgc
     1981 gactgcgcga tttgtccact atccacatat ggtgtggata gtggcaccgg tgcaaactct
     2041 gtgggcgagt tgttgaaatc ccgtacagtg cccctaagtg ctgtgtccac agcggattat
     2101 ccctgagcca cgagccccgc ctcgagcgac cgacgcagct gcgcatcaga aggcgcgacg
     2161 gtccactccc cgacggcgtt cggtcccaac gtccccactc gcgacggcag cgcgaagcgc
     2221 agggagccgc cacggacttt cttgtcgtgt cgcatcgcgt ccaggagcga ttccgcactg
     2281 gccccctcgg ggatcgtcac cggcagtccc tgccgctcga gcgccctggc gatgcgagcg
     2341 gcggtgcctt gctccgctac gccaatcgcc tccgccagcc gcgcctccgc gaccatcccg
     2401 atcgccaccg cctccccgtg cagcaaagcg taaccgctcg tggcctccag ggcgtgccct
     2461 accgtgtggc cgaagttcaa cgaggcccgc cgacccttct cccgctcgtc cgccgccacg
     2521 atctccgcct tgatctcgac cgaccgtgcc accacccgct cgagcgtcgc cgcctcggcc
     2581 gtcaccggga cgggcgagcg ctccagcagg gcgaagtagt ccgcatcggc gatcgccgcg
     2641 tgcttgatcg cttccgccat cccggcggcg agctgcggca gcgcgagcgt cgccaggacg
     2701 tcgagatcgg ccacgacgag gcgcggctga tgaaacgcgc ccagcaggtt ctttccctgc
     2761 ggcgtgtcga cgccggtctt gccgccgatc gaggagtcga tcatggcgag caatgaggtg
     2821 gggacctgca cccaggggac cccgcggtag taggtggccg ccacgaatcc cgctacgtct
     2881 ccggtgacgc cgcctcccac ggcgagcacg gcgccgtcgc gaccgaaatg ggccgcgagc
     2941 atccggtcgc acagcgcggt ccaggtgtcg cgcgtcttgt tccattctcc ggcggggaag
     3001 gtgagcagct cggccaccat cccggcggcg cgcaacgacg tggtgacgcg ctcgccgtac
     3061 accttcgcca cgtgggaatc gccgatcacc gcgtacgcgg cggcgggaca gtgctcggtg
     3121 aggagggcgg gaagccgggg gaggatgccg cggtcgatca ggatgtcgta ggacgcgtcc
     3181 cgcgtttccc gcagcgggac gcggatgctc accacggcag atcgcccgca ccggcgcgcg
     3241 cgttggctgc gcgcgcgagc acgaacagga gatccgagag acggttcagg tagtgacgga
     3301 tggcggggga aaccggagtc tcccggccga gccccaccac ggctcgctcg gcgcgccggc
     3361 acacggtgcg cgcgacgtgg aaggcagccc ccttgggcgc accaccaggc agaatgaatg
     3421 ccttgagcgg cgggagaccg gcctcgagcc ggtcgatggc ctgctcgagc gcggtgacct
     3481 cgccctcgcc gagcggcggg ccgccgaggg ccttcgccag tcgcgccggg tcgggcgtcg
     3541 ccagctccgc gccgatggtg aacaggctgc gctggatcgc ctgcagcagg gcgtcttcga
     3601 aacccggcgg gtccgtggcc cgggcgaggc ccagggccgc gctcagctcg tctacctcac
     3661 cataggccac gacgcgacga tggtccttcg ggacgcgctc gccgccgaac aggcctgttt
     3721 cccctgcatc acctgtcttg gtgtagatct tcaaccttcc tccccgacct caaagatatc
     3781 accatcatcg gcgtcggccc gaccgggttg ttcggcgcgt tctacgccgg catgcggggc
     3841 gcctcctgcc gcctgatcga caacctcgac cgggtgggcg gacatcaatc ccggcaagaa
     3901 agtcgcgccc ggtcactcgg gcaacatggc attgttcggc cagactccgg actgacgggc
     3961 tgtcgcctgt cagctcaccc cagttcggcc ggcagcgatt cagggaagaa gctgcgaccg
     4021 tcccgcatca cctgctcgaa ccgtgccggg tcgtcggcca acgcggcaat ttcgatttcg
     4081 cccaggcagc cgcgcaccag ggcgaacagc tccgccgagt ggacgttcag ccgctggatc
     4141 tcgaaataca ggccggggtt ctcgcgagac acttcggcgg cggtccgtgc ctccttcagg
     4201 aacgtggtgc tcgcaacccg ggccaggtcc tcgtaggatc tgccgctccg cgccagggtc
     4261 gtgaagaaga gaatcgcgac gaggtggctc agtccaagga cgtactgcat gtactcgtcg
     4321 tgccgggcta ccgggagacg agtgatcgtc agggcggtgt cggtgaacaa ggcggccgct
     4381 tcatcggccg ccgtcgcgtt tccgcaatcg cacaccgcca tgatgcgctc ggagagtgtg
     4441 cgtacgccgg ggccgaacag cgggtgaagg ctggccaccc gcaggccgcg tcctgtggca
     4501 ccctcgagca ggtccagcac atggctcttc agactgaacg cgtcggccac cagtccatcg
     4561 ggcgccagcc cgaacacgcg gtcgagcacc tcgcgtccca cggtcagggg cgtggtgatg
     4621 ataatcgcct ccgcggcgtg aaccccggtg gcgagatcgg acactgcggg gaatcccgct
     4681 accggccccg cggggtcgag cgtgctcacg cgatggccct ggcccgcgaa gaattccgcg
     4741 aaccagcggc cgatcttgcc ggccccgccc acgagcagaa tgcgcaagcc gcgggcggct
     4801 cccgttcccg gactcgtctc ctgcacgcgc accgcggcgc cgatcagcag gtgggcgatg
     4861 cgctcggcga gccgctcgtc caggcccgcc gccgccccga ggcgctggaa cctctccagc
     4921 acctccgcct cggtcgagaa gctgcgcacc ggcagccccg ccgcgcgttt cgcttccccg
     4981 acctcggcgg cgagtgcgag ccgcgacgcg acgagttcga ccagggccgc gtccaccgac
     5041 gcaatttccc tgcgcagctc ctccaggctc gcggccacta cttcgccctc ccgatcgcca
     5101 ccaccagcga gtcgatctgc gccagcatcg cccggcccga gacggcgtgc gcgttcgcct
     5161 cgatgctgaa caccaggcgc ccgccgcccg gtcgatcgat gtagcccgac agcgtgttga
     5221 cccggtcgat gctgcccgtc ttcgcgacca ccgcgccctc gagcggcgtg cctacaaagc
     5281 ggcgcagcag cgagcctggc tggccggcgc ggggcatcgc cgcgaggaag gggtcccgct
     5341 tggggtgagc ggccatgtag ctcaggagcc gggtgaacgc ccgtggcgca accaggttgc
     5401 ccgccgagag cccggagccg tcctcgagcg agaaggcgct cgagtcgatg cccaccgagt
     5461 cgaccaggaa gcgacgctcc acctcgagcc cggcgtccca tgacccggtc cccccctgct
     5521 cccggcccag gatcttcagc agcatctcgg cgaaccagtt ctggctggaa ttgaggatcg
     5581 ggaacaggat gtcggcgagg gggcggccgc tgtactccac gagcgccggg gcgcagcagc
     5641 gcgccgcgcg gtaggcgagc gagtcggtgg tgctggtcgc gcccccttcg accgtgatcc
     5701 ccgcccgctc cagcgcgaag gccagggcgc gcgccgcgta gcggttgggg tcgggaagcg
     5761 cgaagctctc ggtccagggc ttccgtcccc gcgccgcggt gccctccgcc cagatgtcca
     5821 tcgtccccgg agcgcggaag aagttatcgc cgatcgagga gccccccccg gccggaccgg
     5881 tgcgggcccg gttctcgaag gtgaacaggc tcagctcggg cgaccaggtg atcgtcggcg
     5941 gccggcccac ggtatccgac ggcgtgatga ggaagtccac gctgttgtcg tggaagccga
     6001 gccccgagac gggcgccgcg taccaccagt tgaggtcgaa cacgttccag gcggggcgca
     6061 cgagctgcgg ctcgaagtag ctgccgtcgc ccacgatgtg cccggtgatg cgggttacgc
     6121 cgtgggcgcg gatcgagtcg gcgatggcgt tgattgcggt gaaggccgag tcgcaggcgc
     6181 cgggggcgag ggtgtcgacg ccgtaacagc gctcggagaa ggtcggatcg ccgcggccgt
     6241 acaggatcag gtcaccgtcg agtatcccat cctgcagcac cccgttgatg tacacgctgg
     6301 tccgcacgcg atagtcggcg ggaagcagca cggtggcggc cgaggtgacg acgagcttcg
     6361 tattgctcgc ggggacgaac agccggtcgg cgttgcgctc gtagagcgtc cgcccccgct
     6421 cgtccacgac cgacacgccc caggtgaccc ggtcgaaggg gcgccggtcg agcagcgcat
     6481 cgagccgccg ggcgagcgac ccgcggcccg gccgggcggc cgtctgggcg acggccgtga
     6541 ggggcgcccc cagggcgcag ctaatcgtga gcgatacgag gatcggcttt cgcatacagc
     6601 aggtccgcga cgaggttgac gaacacgaac acgagggaca tgaacaggat cgcgccctgg
     6661 acggcgggga ggtcgcgctt ctcgatggcc gtcagcacgt agcggccgag ccccggccag
     6721 gcgaagatcg actcggtgag caggctcccc gtcaggtagt tgccggtgtc gagcgcgagc
     6781 accgtgatga ccgggatgag ggcgttgcgc agcgcgtgcc gcgtcaccac cgcgagctcg
     6841 gggagtccct tggcgcgggc ggtccggatg aagtcgctcg ccagcgcctc ccgcatcgcg
     6901 ccgcgggtc
//
LOCUS       DBNGZW010000235        10771 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_225435, whole genome shotgun sequence.
ACCESSION   DBNGZW010000235 DBNGZW010000000
VERSION     DBNGZW010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10771)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10771
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_225435"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="AC16JC_05580"
     CDS             <1..522
                     /locus_tag="AC16JC_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020719897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5164202.1"
                     /translation="EQQPFGELERLVTQKLLDVVRRVAQDELENDDWLRLVARLGNRS
                     PEEMRVIVLEFLDTDVFHLSAEHCNAFLDPLIEAWDIDLETFALDIALNRTVVPGQAK
                     RFEFVEREAGQPSGAEPGLEEFLRDPSLSGSASVEEVEFLKRLKFTDRRPTGLYFYRE
                     LQNLRDPLHFVTA"
     gene            complement(512..2023)
                     /locus_tag="AC16JC_05585"
     CDS             complement(512..2023)
                     /locus_tag="AC16JC_05585"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR5164203.1"
                     /translation="MVSRAYWAIAAGLLTATSVLAQSVQAPQDPAAWSAALAKVSAAT
                     VTLEVQGACCGASGSGLLLTHTGLIATSAHVIAGAQRVRVRLSTGEVFDAVGMLSYTP
                     RLDLALILIPGIALPSAPLADSDSLVVGQRLLAVGVPLGEGTTATETVLRAVRTDDGV
                     RWLHMSVPVFPGAGGGPIVNEQGDVVGLVVVGAAAGGEPLDMAIPINDVRGAVGRLAG
                     RTPTPFAEMTYAGLASVPVGAPSPYEPPIGAAAGAPRPSSDFELDFRPLNGVVLYFEE
                     RYAGLHSVRDSTGYVVSSTPDGRPAVERHNNREWRDQGANAPIAAQQLRTTYVVGASN
                     RSASAFTLRARARAIPTVSGELHIESERYWYSSAEGVHEGQAAPGVAPREMLSAVLAA
                     LPDDLPPEVRVSVLDAEADSTLDAIVRFGGHASLVIPVPLDGRTCAADVPTRSVAVDV
                     VQATRRTGTETVTLVVLARRPHVILGGATAGMTARLTCAVLPAAGAVAASSMP"
     gene            complement(2105..3091)
                     /locus_tag="AC16JC_05590"
     CDS             complement(2105..3091)
                     /locus_tag="AC16JC_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR5164204.1"
                     /translation="MTHIRKNRAPTLVATVAVVGLLAAACGYHTSLASIQVSPDTSVA
                     ARASQQFTAEGRDARGALFAITPTWSVAASGGTISNTGLFTAGPTAGTFLNTVKATDG
                     NISGSASVVVTTPSPVLATITVTPAGPDSLPATTTRQFLAQGKDSAGNSFAFTPAWSI
                     VAGGGTITTAGLFTAGTVPGVFTNTVKASSGTVAGYATVTVASSAVSLATMQELVHFA
                     YDKSDLTADSRAALDAKVKVFQANPAMRILIVGHTDDRGTGAYNLALGTRRAEAVRDY
                     LVAQGVATSRIELDTRGETQPIASGSSEDAMAQNRRGAFRILVAADAPALKQ"
     gene            3275..3586
                     /locus_tag="AC16JC_05595"
     CDS             3275..3586
                     /locus_tag="AC16JC_05595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164205.1"
                     /translation="MTEPSAAREYLADRLDGARDLYLLALALGVRGDGVTLFGRMIRE
                     ARIHFIAVIEECRLAGLDTSAIAAMLGKRNEELVDSIRPEIWAHLERLIARRVRGRAT
                     G"
     gene            3626..3778
                     /locus_tag="AC16JC_05600"
     CDS             3626..3778
                     /locus_tag="AC16JC_05600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164206.1"
                     /translation="MPSILKRGPATTALLLLSCGGTTPIKTLLDDPQQFGGKTRAVGC
                     RGRGPK"
     gene            complement(3861..5525)
                     /locus_tag="AC16JC_05605"
     CDS             complement(3861..5525)
                     /locus_tag="AC16JC_05605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR5164207.1"
                     /translation="MRYAPSLLVLLLFVGLPRPAAAQCDGKTVSAIAIDPHDPSFLSF
                     PRPLRTLARAVGLEHTTTKADVIRRFLLLDVGRSCSATQVAESERILRLQPFLAEATV
                     RAVPDSVGGVRIDVETVDEIPTIFSLPVRSWRPVALRVGNGNVGGQALYVAARVEHGF
                     RYRTGVGLQLVSYQAFGRPYTLAFLAYRAPLGSTLTLALGHAFLTDLQRTAWHVGYSN
                     VNSYTSFLRPDSGVISLGVVRRFADIGSVQRIGFGRHVGFLGGLLTYQRVAPAAQTVV
                     ITDSGLVADTSASISGPFARYHNVRLNAVAGVRTLSFLEVRGFDALAAVQDVARGVQV
                     GVIVGQGNDTFLSTDLYAGLGSARYFAGLRVEAEARHDPGAHRWDSMVASGRLGWYWK
                     PSDAHVVIASDEFSGGWRLRIPFQLSLGALPGGVRGYHDSRVAGAVRNVARVEGRWPL
                     GQVTRYVGLGLASFADAGMIWPGDAPFGLQSGVKTGVGAGLLVAVPPQSKQLWRLDFA
                     APVSPDAHARWEVRLSAVWMGGFWSEPRDVAQARSGAAPSLIFTWP"
     gene            5940..6386
                     /locus_tag="AC16JC_05610"
     CDS             5940..6386
                     /locus_tag="AC16JC_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR5164208.1"
                     /translation="MLDLLYQLESRMGRSFGEPQRTFEWPVEAATTAWLPLVDVFEEP
                     DVIRLVAEVPGVRPEDVKISVDGNLLTIKGTKEQVAEEKAEKVHHYERSYGAFERTFR
                     LSASIDPNKIKATYDLGVLTITLPKAETAKPHLIKVEVTPEKALKA"
     gene            6478..7902
                     /locus_tag="AC16JC_05615"
     CDS             6478..7902
                     /locus_tag="AC16JC_05615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HIR5164209.1"
                     /translation="MNTRQVLAAAITAASAVGMPAHRPPVAAALRAPADTLREPPLLR
                     NRSRVPGVVEFELTAAPDKLQLLPGGPAVDAYAYNGTVPGPTIDVREGDSVVIHFHNA
                     LPDTSTVHWHGLHLPAGMDGSVLDPIPPGATHDYAFRLARGSAGTYWYHPHPDVHSTY
                     QVAMGLFGAFIVRAADDPLAARGTPEKLLILSDNRFDADGRVAFPDPRSIAAEVDLEN
                     GREGDVLFVNGQVSPTIPIRRGEVQRWRIVNASAARVYRLALPGRSLIKVGTDGGLFE
                     HPVEVPDVVLANSGRVELLVRGDSSTALQDLPHDRYMPQTRPADWDRTRTLLAVHVTG
                     RVAVRPVAIPPVLRPVPALDTTQVAARRVIVMSQGQLDSKRMDMHRIDIQARMNTTEI
                     WTIQNVVGMDHPFHLHGFQAQVLDRNGVPEPFPSWNDTVNVPKHSSVRIVVRFVDYPG
                     RWMYHCHILDHEDEGMMGVLELSN"
     gene            7914..8588
                     /locus_tag="AC16JC_05620"
     CDS             7914..8588
                     /locus_tag="AC16JC_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164210.1"
                     /translation="MKSGFALPALALVAAVGLLGARAARLGPEPRSALAGATPADTVA
                     RGRYLVISHDCGGCHGGGADPSAAGWLAGETTPDMQFLIGPCYLTPGAKPCFHTHPRN
                     LTPDNETGLGRFTERQIFNALRYGLRPEDTPDVDITATTPGMGNFPLHPRYLAVPMPW
                     NAWRYMPDEDLWAIAAYLKRGVKPVHNMVQESEGPPDFWASEYTVAKIGAFPVASFPT
                     KNEQMP"
     gene            complement(8869..>10771)
                     /locus_tag="AC16JC_05625"
     CDS             complement(8869..>10771)
                     /locus_tag="AC16JC_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164211.1"
                     /translation="ITRLTPGEISISRGITAVTQRYATTVQVSRRGNTLQFRAPPAEL
                     LSDIRLGALLLLPDCEGAIEPDDVFRLIEASSAFPGAFSPVLLSHQPSPGAGCGAGPP
                     ADTTALFSDGGLFDNNPIDLVAGIHADAVWKHRDRPDPNALLVFIDPEALRGRLALVE
                     KQRKREQPATGGISALVDLFAGAVPAARQYELQAFARLLARAPQVFRRDNIEVTNRRF
                     VVVGQQLGAFAAFLGQPFREYDFYVGVYDALTFMAREGCRTAQGVDSACVAGRLRTLV
                     ESRQLDLGEGELPRVMLRALYEREFLTDSASSSRNELLRAQPLPVQSPRPLLLRALLE
                     AHLALDARPPFDNDGCVGDAVTRLLCRDGFREIIGRVAADSGVRAILDTAERRPECAP
                     ENWVESPTRCDASQSLLAFVDDPERFMAGKIGLMLHQMWRVEQARRQAGEPAWTNVAT
                     LSEALYQSGIAYRYRRGPEPNTSSVPRGSGRGWIASLVPNYANVSFLNAAFEVGYRPT
                     WHVGRSVALGLTAAPLHVTGGAATSIDRVHFVIGPTLHWKRQSPVFSGLETGLEYFGN
                     WRPRPATNPEKTVWAVPLTAYLLADKLRIGFRVLPGNTSAVHGGKKVALTFGLADLNG
                     LVYWMLRKS"
BASE COUNT         1821 a         3625 c         3715 g         1610 t
ORIGIN      
        1 gagcagcagc cgttcggcga gctggaacga ctggtcacgc agaagctgct ggacgtggtc
       61 aggcgcgtgg cccaggacga gctcgaaaac gacgactggc tccgcctggt ggcgcggctc
      121 ggcaaccgca gccctgaaga gatgcgggtc atcgtactcg aatttctgga tacggatgtg
      181 tttcacctgt ccgccgagca ctgcaatgcg tttctggatc cgctgatcga agcctgggat
      241 atcgacctgg agacgttcgc gctggacatc gccctgaacc gcaccgtcgt gcccgggcag
      301 gcaaaacgat tcgagttcgt cgagcgggaa gccgggcagc cgtccggcgc cgagccggga
      361 ctcgaggagt ttctgcggga tccctcgctc agcggcagcg cgagcgtgga ggaagtcgag
      421 ttcctgaagc ggctgaagtt cacggaccgg cggccgacgg ggttgtactt ctatcgggag
      481 ctgcagaacc tgcgggaccc gctgcatttc gtcacggcat agacgacgct gctacggcgc
      541 ccgccgctgg cagcacggcg catgtcaggc gcgccgtcat gccggccgta gccccaccca
      601 gaatgacgtg cggacgccga gccaacacca cgagtgtgac cgtttccgtt cccgtgcggc
      661 gcgtggcctg gacgacgtcg accgcgacgc tccgcgtcgg gacatccgca gcgcacgtcc
      721 gcccgtcgag cgggacagga atcaccaggg acgcatgtcc gccgaaccgc acgatggcat
      781 ccagggtgct gtccgcctcg gcgtccagca ccgacacgcg gacttcaggc ggaaggtcgt
      841 cggggagggc cgccagaacc gcggagagca tctcccgtgg agcgaccccc ggcgcggcct
      901 gaccctcgtg aacgccttcc gcggagctgt accagtatcg ttcgctctcg atgtgcagct
      961 cccccgaaac ggtcgggatc gcccgggcgc gagccctcag ggtgaacgcc gaggccgacc
     1021 ggttggacgc gcctaccacg taggtcgtgc ggagctgctg cgcggcgatc ggcgcattcg
     1081 ctccctggtc ccgccattcc ctgttgttgt gccgctccac tgccggcctg ccgtccggcg
     1141 tcgaggacac gacgtagccg gtgctgtcgc ggacgctgtg caggccggca taccgctctt
     1201 cgaagtagag gacgacaccg ttcaagggac ggaaatccag ctcgaaatcg gagctgggcc
     1261 gcggcgcacc ggcggcagca ccgatcggcg gctcgtacgg tgacggcgct ccgacgggca
     1321 cggaggccag tcccgcatag gtcatctccg cgaacggcgt cggtgtcctc cccgccaggc
     1381 gccccaccgc accgcgcacg tcgttgatcg ggatggccat gtcgagcggt tcgccgccgg
     1441 cggctgcgcc gaccacgacg agccccacca cgtcgccctg ctcgttcacg atgggaccgc
     1501 caccggcgcc cgggaacacc gggacggaca tatggagcca gcgaacgccg tcgtcggtgc
     1561 gcaccgcacg aagcactgtc tccgtcgccg tcgtgccctc gccgaggggc actccgacgg
     1621 cgagcaaccg ctgccccacc accaggctgt cggagtcggc gagcggtgcg ctcggcaacg
     1681 cgatccccgg gatgaggatg agggccagat cgaggcgagg cgtataggac agcatcccca
     1741 ccgcgtcgaa cacttcaccg gtggagagcc gcacgcgcac ccgctgcgct cccgcgatga
     1801 cgtgcgcgct ggtcgcgatg agacccgtgt gggtcagcag caggccgctg ccgctcgctc
     1861 cgcagcaggc gccctgcacc tcgagggtca ccgtggccgc gctgactttg gcgagcgccg
     1921 cggaccatgc ggcggggtcc tggggggcct ggacgctctg cgcgagcacg ctcgtcgccg
     1981 tgaggagacc ggcggcgatc gcccaatacg ccctggatac catggagcgg aaggtcatgc
     2041 cgcaaggggc gggcaaaagc cgggcgcgat cttccgcgcc cggccttgtc ctgccggtct
     2101 tcacttattg cttcagggcc ggtgcgtctg cggccacgag aatccggaac gcgccacgcc
     2161 ggttctgcgc catggcatct tccgagctgc ccgacgcgat gggctgcgtc tcgccacggg
     2221 tgtcgagctc gatccggctc gtcgccacac cctgcgcgac caagtagtcc ctgaccgctt
     2281 cggcacgccg ggtgccgagc gcgaggttgt acgcgccggt gccgcggtcg tcggtgtgcc
     2341 ccacgatgag aatacgcatc gccgggttgg cctggaacac cttgaccttg gcgtcgagcg
     2401 cagcgcggga atccgcggtg agatccgatt tatcgtaggc gaagtgcacc agctcctgca
     2461 tcgtagcgag cgacacggcg gaggacgcca ccgtcacggt cgcgtacccg gcgacggtgc
     2521 cggagctcgc cttgaccgta ttggtgaaca ccccgggcac cgtgcccgcg gtgaacagcc
     2581 ccgccgtggt gatggtgccg ccaccggcga cgatggacca ggcgggcgtg aacgcgaagc
     2641 tgttgccggc ggagtccttg ccctgggcca ggaactgccg ggtcgtcgtg gccgggaggg
     2701 agtccgggcc cgctggagta acggtgatgg tcgccagcac gggcgaggga gtggttacga
     2761 ccaccgacgc gctgcctgag atgttgccgt ccgtggcctt gaccgtattg aggaaggtcc
     2821 cggccgtcgg acccgcggtg aagagccccg tgttgctgat ggtgccacca ctcgccgcga
     2881 cggaccaggt gggcgtgatc gcgaagaggg cgccgcgggc gtccctgccc tcggcggtga
     2941 actgctggct ggcgcgggcg gctacgctcg tatcgggtga gacctgaatg gacgccagac
     3001 tggtgtggta cccgcacgcc gccgccagta gcccgacgac cgccaccgtg gcaacgaggg
     3061 ttggtgcgcg gttcttcctg atgtgtgtca taggactgct cctaaccgat ttcgtgtgat
     3121 gaggaccttc tggtgcaacc gcctcccgga ctgctgcgga gaaaggcgcc tacgcagcct
     3181 accgcatgtg cgcgcggcga gctatccatc gtaggagtga cggcgaaatc tccatgctcg
     3241 gccgatgcgg ccgcaccatg acaggagtaa ggcgatgact gaaccttccg ctgcgcggga
     3301 atacctcgct gaccggctcg acggcgcccg ggacctctat ctgctcgccc tcgcgctggg
     3361 cgtacgcgga gacggcgtga ccctgttcgg ccgaatgatc cgcgaagccc ggatccattt
     3421 catcgccgtg atcgaggagt gccgtctggc ggggctcgac acgagcgcga tcgccgcaat
     3481 gctcgggaag cggaacgagg agctggtgga cagcatccgg cccgagatct gggcgcatct
     3541 ggagcggctc atcgcgcggc gggtgcgggg tcgcgcgaca gggtgaacgc tctggctcaa
     3601 ggacgacatt cagggagctt cgatcatgcc aagcatactg aaacgggggc ccgcgacgac
     3661 cgcgctcctg ttgctcagtt gtggcggaac cacgccgatc aagacgctgc tcgatgatcc
     3721 gcaacagttc ggcggcaaga cgagggcggt ggggtgccgc ggcagggggc caaagtaggg
     3781 ctcggatccc agagcgtggc cgtggtggtg gaacagcgcc gttacactcc ctaaacggag
     3841 tttgatcaag cacgtgagga tcacggccac gtgaagatga gggagggggc cgcgccggac
     3901 cgcgcctgtg ccacgtcccg cggctcgctc cagaatccac ccatccacac ggccgacaac
     3961 cggacctccc agcgcgcgtg cgcgtcgggg ctcacaggag ccgcgaaatc caggcgccag
     4021 agctgtttgg actgcggcgg gacggccacc agcagtcctg cacccacccc cgtcttcacc
     4081 ccgctctgca agccgaatgg tgcatcgccc ggccagatca tccccgcgtc ggcgaagctc
     4141 gcgagcccga gcccgacgta ccgggtgacc tgcccgagcg gccagcgacc ctcgacgcgc
     4201 gcgacgttcc gcaccgcgcc ggcgacgcgc gaatcgtggt acccccgtac cccgcccggc
     4261 agggctccaa gcgagagttg aaagggaatc cgcaggcgcc acccaccgga gaattcgtcg
     4321 ctggcgatga cgacatgagc gtcggagggc ttccagtacc atcccagccg accgctggcg
     4381 accatcgaat cccaccggtg cgcgcccgga tcgtggcgcg cctcagcttc gacgcgcagc
     4441 ccggcgaagt accgcgcgga acccagcccg gcgtagaggt cggtggagag aaaggtgtcg
     4501 ttcccctgcc ccacgatgac tccgacctga acgcctctgg ccacatcctg caccgccgcg
     4561 agcgcgtcga acccgcgcac ctccaggaac gagagggtgc gcacgccggc gacggcgttc
     4621 aggcgcacgt tgtggtagcg agcgaacggg ccgctgatcg acgcgctcgt gtcggcgacg
     4681 agtcccgagt cggtgatgac gaccgtctgg gccgccggcg cgacgcgttg atacgtcagc
     4741 agaccgccga ggaacccgac atgccgtccg aagccgatgc gctggacgct cccgatgtcc
     4801 gcgaagcggc gcaccacgcc gagcgaaatg actccgctat ccggccggag gaacgacgta
     4861 taggaattca cattgctgta accgacgtgc cacgcggttc gctgcaggtc ggtgaggaag
     4921 gcgtggccga gggcgagcgt cagagtgctg ccgagcggcg cgcggtaggc caggaacgcg
     4981 agcgtgtatg gtcggccgaa cgcctggtag gagacgagct gcaggcccac gccggtgcgg
     5041 tagcggaacc cgtgctccac tctggccgcg acgtatagcg cctggcctcc cacattgccg
     5101 ttgccgacgc ggagtgccac gggacgccag ctcctcacgg gcagcgagaa gatggtcggg
     5161 atctcgtcga cggtctcgac gtcgatgcgc acgccgccga cggagtccgg caccgcgcgg
     5221 accgtcgcct cggcgaggaa gggctggagg cggagaatgc gctccgattc cgccacctgg
     5281 gtggccgagc aggaccgccc gacatccagg agcagaaagc ggcgaattac gtccgctttc
     5341 gtggtcgtgt gctccaaacc aacggcgcgc gcgagcgtgc ggagcgggcg gggaaagctg
     5401 aggaacgacg ggtcatgagg gtcgatggcg atcgccgaga cggtcttgcc gtcacactgt
     5461 gccgcggcgg gcctcggtag ccccacgaac aggagcagca cgaggagaga cggagcgtag
     5521 cgcacgagat cacggagacc gataatggtg cgccggtcgc gacatcgcgg ttggcccgat
     5581 cgccaatcct gacccgatag cgctccgcga cggcgcgcgc gccacgagcg ccagcatgca
     5641 ttcgcttgcc tgtcgcgctc caacttttgc agcgagctag acgaattgat cgcaggtcta
     5701 tggtatccgc gaccggggcg cccagcaaac ttgcacctct ctcctatccc tctgttgtcc
     5761 tcgccccccc tgccccgctc accgtcatcg caactttact agattagcta agtggcatac
     5821 caacatggaa cacctgactt gacaaactta gtacattagt gtaacctgca gccagttgga
     5881 atgcccaagt agttcactgg aaggagcacg atatgtatag tcttaccaag agggacccga
     5941 tgctggacct gctgtaccag ctggagagcc ggatgggccg aagcttcggc gagccgcagc
     6001 gcacgttcga atggcccgtc gaagccgcga cgaccgcctg gttgccgttg gtggacgtct
     6061 tcgaagagcc ggatgtcatc cgcctggtgg cggaagtgcc cggggtcagg cccgaggacg
     6121 tgaagatctc ggtggacggc aacctgctga cgatcaaggg cacgaaggag caggtggcgg
     6181 aggagaaggc cgagaaggtg catcactatg agcggagcta tggcgccttc gagcggacgt
     6241 tccggctgtc ggcctcgatc gatcccaaca agatcaaggc gacctacgac ctcggcgtgc
     6301 tgacgatcac gctgccgaag gcggagacgg ccaagcccca tctgatcaag gtggaagtga
     6361 cgccggagaa ggcgttgaag gcctgacgtc acttgaagtg aatgagggcg gccctggttg
     6421 gccgcccttc tcacgcgcct ccctcacacg acgcagggta ccctttttgg acgcagcttg
     6481 aatacgcgac aggttctggc cgccgccatc accgctgcgt ccgcggtggg catgcccgcc
     6541 caccgtccac ccgtcgccgc ggcgctgcgc gcgccagccg ataccctgcg cgagccgccg
     6601 ctgctgcgca accgctcgcg cgttccgggc gtcgtcgaat tcgagctgac ggcggcgccg
     6661 gacaagctgc agctgctgcc cggcgggccg gcggtcgacg cctacgccta caacggcacc
     6721 gtgcccggcc cgacgatcga cgtgcgcgag ggcgacagcg tcgtcatcca cttccacaac
     6781 gcgctgcccg atacgtccac ggttcactgg cacgggctgc acctgccggc gggaatggac
     6841 ggcagcgtgc tcgatccgat cccccccggc gcaacgcatg actatgcctt ccgcctcgcg
     6901 cgcggctcgg ccggcaccta ctggtatcac ccccaccccg acgtgcactc gacttatcag
     6961 gtggccatgg gtctgttcgg cgccttcatc gtgcgcgcgg cggacgatcc cctcgccgcc
     7021 cgggggacac ctgaaaagtt gctgattctt tccgacaacc gcttcgatgc cgacgggcgg
     7081 gtcgcgttcc ccgacccgcg atcgatcgcc gcggaagtcg atctcgagaa tgggcgcgag
     7141 ggcgacgtcc tgttcgtcaa cggccaggtg tcgcccacga tcccgatccg acgaggtgaa
     7201 gtgcagcggt ggcgcatcgt gaacgcttcg gcggcgcggg tgtaccggct ggcgttgccg
     7261 gggcggtcgc tgatcaaggt cggcaccgac ggcggcctgt tcgaacaccc ggtcgaggtg
     7321 cccgacgtgg tgctcgcgaa cagtggccgg gtggagctgc tggtgcgagg cgacagctcg
     7381 accgcgctcc aggatctccc ccacgatcgg tacatgcccc agacgcgacc ggcggactgg
     7441 gaccgcacgc gcacactgct cgccgtccac gtgactggcc gagtcgcggt ccggcccgtc
     7501 gcgatcccgc ccgtgctgcg ccccgtcccc gccctggata cgacgcaagt cgccgcacgg
     7561 cgcgtgatcg tcatgtcgca agggcagctg gacagcaagc ggatggacat gcaccgcatc
     7621 gacatccagg cgcggatgaa cacgacggag atctggacca tccagaacgt ggtgggaatg
     7681 gaccatccgt ttcacctgca cgggttccag gcccaggtgc tcgaccgcaa cggcgtgccg
     7741 gagccgttcc cgagctggaa cgacaccgtc aacgtgccca agcactcgtc ggtgcgcatc
     7801 gtggtgcggt tcgtcgatta cccggggcgc tggatgtatc actgccacat cctggatcac
     7861 gaggacgaag gaatgatggg cgttctcgaa ctctccaact gaagggagcc gtcatgaagt
     7921 cagggtttgc attacccgcg ctcgcgctgg tcgcggcagt ggggctgctg ggcgcgcgcg
     7981 cggcgaggct cggtcccgag ccacgctccg ccctggcggg cgcgacgccg gccgataccg
     8041 tcgcccgcgg ccggtatctc gtcatctcac acgactgcgg cggctgccat ggcggcggcg
     8101 ccgatccctc ggccgccggc tggctggcgg gcgagacgac gcccgacatg cagttcctca
     8161 tcggcccctg ctatctcacg ccgggggcga agccgtgctt ccacacccat ccgcgcaacc
     8221 tgacgccgga caacgagacc ggtctgggcc gcttcaccga gcgccagatc ttcaacgcgc
     8281 tgcgctacgg gctgcgaccg gaggacacac cggacgtgga catcactgcc accacgccgg
     8341 gcatgggcaa tttcccgttg cacccacgct acctcgcggt gccgatgccg tggaatgcgt
     8401 ggcgctacat gccggacgag gatctgtggg ccatcgcggc ctacctgaag cggggcgtca
     8461 agccggtgca caacatggtc caggagagcg aggggccgcc ggatttctgg gcaagcgaat
     8521 acaccgtcgc gaagatcggc gcgtttcccg tcgcgtcgtt tccgacaaaa aacgagcaga
     8581 tgccctaggt cctctccccc tggccccgct ccgcggatag gtacctcacc ccggataggt
     8641 gcctcacccc caggcgccct ttccgcttga cgcggagagg gggaaccaaa tgcaaggatc
     8701 gtttgatctc ccgcgccgtt tccccctccc ccgaaaggga gtgggggaca gggggtgaga
     8761 tacaaatgag gatcgcttga tctcccacgt cgttgcccct ctcccgtaag ggagaggggg
     8821 acagggggtg agataccaag aagagaagaa gaaaggagag gggcagggtc acgacttgcg
     8881 cagcatccag tagaccaggc cgttcaggtc cgcgagcccg aaggtcagcg ccaccttctt
     8941 gccgccatgc acggcggagg tattgccggg gagcacccgg aagccgatgc gcagcttgtc
     9001 ggccagcagg taagcggtca gcggcaccgc ccacaccgtc ttctccgggt tcgtggccgg
     9061 gcggggacgc cagttcccga agtactcgag cccggtttcc agccccgaga agacggggct
     9121 ctggcgcttc cagtgcagcg tcggtccgat cacgaagtgg acccggtcga tgctcgtggc
     9181 ggctcctccc gtcacgtgca gcggcgcggc ggtgaggccg agggcgaccg accgccccac
     9241 gtgccaggtc ggccggtacc ccacctcgaa ggctgcgttc aagaagctca cgttggcgta
     9301 gttgggaacg agcgaggcga tccagccgcg acccgacccc cgcggcaccg acgacgtgtt
     9361 gggttccggg ccgcggcggt agcggtacgc gatgcccgac tggtacaggg cttcgctcag
     9421 ggtcgcgaca ttggtccacg ccggctcccc cgcctggcgc cgcgcctgct ccacgcgcca
     9481 catctgatgc agcatgagac cgatcttgcc cgccatgaag cgctccgggt cgtcgacgaa
     9541 ggccagcagg ctctgactgg cgtcgcagcg ggtcggagac tcgacccaat tctcgggcgc
     9601 gcattccggc cgccgctctg ccgtgtcgag gatggcgcgc acacccgagt cggcggccac
     9661 gcggccgatg atctcacgaa agccgtcccg gcacagcagc cgggtgaccg cgtccccgac
     9721 gcagccgtcg ttgtcgaacg gcggccgggc gtcgagcgcc aggtgtgctt ccaggagcgc
     9781 acggagcagg agaggacggg gggactgaac ggggagcggt tgggcacgga gcagctcgtt
     9841 gcggctcgaa gaggcggaat cggtgaggaa ctcccgctca tacagcgcgc gcagcatcac
     9901 gcgcggcaac tcgccctcgc ccaggtccag ttgccgtgat tccacgagcg tgcgcagccg
     9961 gccggccacg catgccgaat ccacgccctg ggccgtccgg cagccttcac gtgccatgaa
    10021 cgtgagcgcg tcgtacacgc cgacgtagaa gtcgtattcg cggaacggct gccccaggaa
    10081 cgcggcgaac gcacccagct gctggccgac cacgacgaag cggcgattgg tgacctcgat
    10141 gttgtcgcgc cggaatacct ggggagcgcg ggcgaggagt cgcgcgaagg cctgcagctc
    10201 gtactggcgg gcggcgggca ccgcgcccgc gaacaggtcc accagggcgg agatgcctcc
    10261 ggtggccggc tgctcgcgct tgcgctgctt ctcgaccagc gcgaggcggc cgcgcagcgc
    10321 ttcggggtcg atgaacacca gcagcgcgtt ggggtcgggg cgatcgcggt gcttccagac
    10381 cgcgtcggcg tggatccccg ccacgaggtc gatggggttg ttgtcgaaca ggccgccgtc
    10441 ggagaacagc gcggtggtgt ccgcaggggg gccggcgccg caccccgccc ccgggctcgg
    10501 ctggtggctc agcaagaccg gtgagaaggc gccggggaac gcggaagacg cttcgatcag
    10561 ccggaagacg tcgtccggct cgatggcacc ctcgcaatcg ggcaggagca gcagggcgcc
    10621 gagccggata tcgctcagca gctcggctgg tggcgcgcgg aattgcagcg tgttgccccg
    10681 gcgactcacc tgcaccgtcg tggcgtagcg ctgcgtcacc gcggtgatgc cgcgcgagat
    10741 cgagatctcc ccgggcgtga gcctggtgat g
//
LOCUS       DBNGZW010000236         3983 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_21133, whole genome shotgun sequence.
ACCESSION   DBNGZW010000236 DBNGZW010000000
VERSION     DBNGZW010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_21133"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="AC16JC_05630"
     CDS             complement(<1..865)
                     /locus_tag="AC16JC_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164212.1"
                     /translation="MRAVRSLTVAGFGLWLAACQPQARRLLLLDEASSQPAELEATAR
                     PWRDAGYHVEYRRYYPHLTRADLDRYRLVMVLGGRGANSTADALDVGDLSLLTEWTLR
                     GGVVVLGYPPNGEGGGGNAFDRWLMNRWLAWSGAGIAIGAVALRGGAPQTAPEVSPVL
                     TAGLRGTGYVPFPAGTNNALLVDDEAQVLARAVDGAPSQAPEQDGRDGRRRGVPVAAA
                     SRTGNGLVVVVSRSALGAVRQEDSLNRTGTRAFLVALARFTRRPAEWARIPPAGRRSP
                     LRLTGGPQPVIV"
     gene            complement(844..2415)
                     /locus_tag="AC16JC_05635"
     CDS             complement(844..2415)
                     /locus_tag="AC16JC_05635"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional serine/threonine-protein
                     kinase/formylglycine-generating enzyme family protein"
                     /protein_id="HIR5164213.1"
                     /translation="MTDPLFAQVATALGDRYTLVRELGQGGMGAVFLGRDVKLGREVA
                     IKVLPPTTRAYLGSDRFQREVQLAARLSHPHIVPLFEADEVDGFLYYVMGYVAGESLA
                     ERLTKQGQLPLEEAIRITTEVGDALQYAHAHGIIHRDIKPANILLSGGHALVTDFGIA
                     KSVAESEEGKTLTGTGVTVGTAAYMSPEQASGERRIDARSDVYALAAVLYEMLTGEPP
                     FTGATAQAIVARILVDAPRPVRTVRPNVPAHIEQALLVGLAKVPGDRPPSAKAFIDLL
                     SRPVTAPPAPRRWLRYAAGAAVLGATAMAVALWTRPRAPAATARPVGMALVPADTYPV
                     GGGRGGRPGHGVVLDAFYLDSTEVTVAAYQRYLDSTASAAPWSRRPPGTWPATGVLWS
                     EAQRYCTSRGGRLPTEDEWEAAARSRDGRRYPWGDRWERGRANAGSMTDTLQPVGAFP
                     LGRSPAGAEDMVGNAWEWTANDTVTPTGQVLHVIKGGAFNTAPENATATFRSALPDVR
                     PLLWNTGFRCAQSVR"
     gene            complement(2412..3263)
                     /locus_tag="AC16JC_05640"
     CDS             complement(2412..3263)
                     /locus_tag="AC16JC_05640"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5164214.1"
                     /translation="MPRRDLIDQVRKALGHRYEIVTAVGRGGNASIFGAFDKAGNRVA
                     VKVLHPELAASVAADRFLREIRYAGKLHHPHIAPLLDSGETDYLLWFVMPYVPGESLR
                     RALQRERRLAVDFAARAGCEILEALDHAHSKGLAHRDIKPDNIVLSPDGAVLVDFGIA
                     RAIAQSEGDRVTRSGFVVGTEEYMSPEQASGAEEVDGRTDLYSLGVVLFEAIAGRPPF
                     SAASAAAVIDMQQHHAPPSLQSLRPEVAQGLATVIGRALEKRPADRWQSAAEMRQALL
                     PYSLAAE"
     gene            3367..3885
                     /locus_tag="AC16JC_05645"
     CDS             3367..3885
                     /locus_tag="AC16JC_05645"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HIR5164215.1"
                     /translation="MSHLEAVRGDITEQAVDAIVNAANTALLGGGGVDGAIHRAAGPE
                     LLAECRGLGGCPTGEARITKGYRLPARFVIHTVGPVWAGGTGGEPELLRRCYASAMQL
                     AADHGVRSIAFPAISTGAYGYPIESAARIAVTAVREALALPTSVQTVRFVCFGDRDWN
                     VYLELLGHRRDC"
BASE COUNT          562 a         1376 c         1449 g          596 t
ORIGIN      
        1 gcacgatcac cggttgcgga cccccggtga gacgcagcgg ggagcgtcgg ccggcgggcg
       61 ggatccgggc ccactcggcg gggcggcgag tgaaacgggc gagggcaacg aggaatgcgc
      121 gcgtgcccgt gcgattcagc gagtcttcct ggcgaaccgc tcccagcgcc gaccgagaga
      181 cgacgaccac cagcccgttc ccggtgcggc tggccgccgc gacaggaaca ccgcggcgac
      241 gcccatcgcg gccgtcttgt tccggggcct gtgacggagc gccgtccacg gcgcgggcga
      301 ggacctgcgc ctcgtcgtcg acgagcagcg cgttgttggt gccggcgggg aacgggacat
      361 accctgtccc gcgcagcccg gcggtgagca ccggactcac ctccggtgcg gtctggggtg
      421 cgccgccccg cagtgcgacg gcgccgatcg cgatccctgc tccgctccac gccagccagc
      481 ggttcatgag ccagcgatcg aacgcgttgc cgcctccgcc ctctccattc gggggatacc
      541 caagcaccac gacgccaccc cgcagcgtcc actcggtgag gagggagagg tcgccgacat
      601 ccagggcgtc cgccgtggag ttcgccccgc gcccgccgag caccatcacg aggcgatatc
      661 ggtcgaggtc cgcgcgcgtg aggtgcggat agtatcggcg gtattccacg tggtagcccg
      721 cgtcgcgcca ggggcgggcg gtcgcctcga gctcagcggg ctggctcgag gcctcgtcga
      781 ggagcagcag gcggcgggcc tgcggctggc aggcagcgag ccacaggccg aagccggcca
      841 cggtcagcga acggactgcg cgcatcgaaa cccggtgttc cacaacagcg gtcggacgtc
      901 gggaagggcc gaccggaagg tggccgtggc attttcgggc gccgtattga acgctcctcc
      961 cttgatcacg tgcaggacct ggccggtggg ggttacggtg tcattggcgg tccactccca
     1021 ggcgttcccg accatgtcct ccgccccggc cggtgagcgg cccagcggaa acgcgcccac
     1081 cggctggagc gtgtccgtca tcgagccggc gttggcgcgt ccgcgctccc agcgatcgcc
     1141 ccacgggtac cggcggccat cgcggctgcg ggcggctgcc tcccattcgt cttcggtggg
     1201 caaccgcccg ccgcgcgacg tgcagtatcg ctgtgcttcg ctccacagaa ctccggtagc
     1261 cggccaggtc ccgggcggcc gcctgctcca cggcgcggcg gaggcggtcg agtcgagata
     1321 ccgctggtag gctgcgaccg tcacttcggt cgaatcgagg tagaaggcgt cgagcacgac
     1381 gccgtggccc gggcgaccgc cccgaccgcc gcccaccggg taggtgtccg cgggcaccag
     1441 cgccatccct acggggcggg ccgtcgccgc cggtgcacgg gggcgggtcc acagcgcgac
     1501 cgccatcgcc gtcgctccga gtacggcggc gccggccgca tagcgcagcc agcgcctcgg
     1561 ggcaggcggc gcggtgaccg gacgcgacag cagatcgatg aatgccttgg ccgacggtgg
     1621 ccggtcgccc gggaccttgg cgaggcccac gaggagcgcc tgctcgatgt gcgccgggac
     1681 gttcggccga accgtgcgca ccggacgggg ggcatccacc aggatgcggg cgacgatcgc
     1741 ctgggccgtg gccccggtga agggcggctc ccccgtgagc atctcgtaca gcaccgcggc
     1801 cagggcgtac acgtcgctgc gcgcgtcgat acgccgttcg ccgctcgcct gctccgggct
     1861 catgtaggcg gcggtgccca cggtcacgcc cgtgcccgtg agggtcttgc cctcttccga
     1921 ctcggccacg ctcttggcga tgccgaagtc ggtcaccagc gcgtgcccgc ccgagagcag
     1981 gatattggcc ggcttgatgt cgcggtggat gatgccgtgc gcgtgtgcgt actgcagcgc
     2041 gtcacccacc tcggtggtga tgcggatcgc ctcctcgagc ggcaactgtc cctgcttcgt
     2101 caggcgctcg gcgagtgact ctccggcgac gtaccccatc acgtagtaca ggaacccgtc
     2161 gacttcgtcg gcttcgaaca agggcacgat gtgcgggtgc gacagcctgg ccgcgagctg
     2221 cacttcgcgc tgaaagcggt cgctgccgag ataagcgcgc gtcgtgggtg gcagcacctt
     2281 gatggccact tcgcggccca gcttcacgtc gcggccgaga aacaccgcgc ccatcccgcc
     2341 ttggccgagc tcgcggacca gcgtgtagcg gtcgcccagt gccgtggcga cctgggcgaa
     2401 cagggggtcg gtcattccgc cgcgaggctg tacggcaaca acgcctgccg catctccgcg
     2461 gcgctctgcc agcggtccgc gggccgtttc tcgagggcgc gaccgatgac cgtggcgagc
     2521 ccctgcgcca cttccggtct caacgactgc agcgagggcg gggcgtgatg ctgctgcatg
     2581 tcgatcacgg ccgcggcgct ggcggcggag aacggcggcc ggccggcgat cgcctcgaac
     2641 aggacgactc ccagcgaata gaggtcggtg cgcccgtcca cctcctcggc gcctgaggcc
     2701 tgctcgggac tcatgtattc ttccgttccc acgacgaatc cgctgcgcgt cacgcggtcc
     2761 ccttcggatt gggcgatggc gcgggcgatc ccgaaatcca cgaggacggc tccatcgggc
     2821 gagagcacga tgttgtccgg tttgatgtcg cgatgggcca ggcccttcga gtgcgcgtgg
     2881 tccagcgctt cgagaatctc gcagccggcc cgggccgcga aatcgacggc gaggcggcgc
     2941 tcgcgctgca gtgcccggcg cagcgactcg ccgggcacgt acggcatcac gaaccagagc
     3001 aggtagtcgg tctccccgct gtccagcagc ggggcgatat gcgggtgatg gagcttgccg
     3061 gcgtagcgga tctcccgcag gaaccggtcg gcggcgaccg atgccgcgag ctcgggatgc
     3121 agcaccttga cggccacgcg attgccggct ttgtcgaacg ccccgaagat cgaggcgttg
     3181 ccgccgcgac cgacggccgt gacgatttcg taacggtgcc ccaacgcctt gcgaacttga
     3241 tcgatgaggt cccgacgcgg catcgcccct gcttggtacg aacgagcgta agatgcggtt
     3301 gccccgtgtc tcgcggaagg taggttgggg cgtggtacac gttctatccg ctgaacggaa
     3361 gggtccatga gtcacctcga ggcggtgcgc ggcgacatca ccgagcaggc ggtggacgcg
     3421 atcgtgaatg cggccaatac ggcgctgctg ggtggcggcg gcgtggacgg ggcgattcac
     3481 cgggccgcgg ggccggagct gctggcggaa tgccgcgggc tcggcgggtg tcccacgggt
     3541 gaggcccgga tcaccaaggg ctatcgcctg cccgcgcgat tcgtgattca taccgtcggc
     3601 ccggtgtggg ccggtggcac cggcggcgag ccggagctgc tgcgccgctg ctacgcgagt
     3661 gcgatgcagc tcgcggccga tcacggtgtc cgctccatcg cctttcccgc gatcagcacc
     3721 ggcgcgtacg gctaccccat cgagagcgcc gcgcgcatcg ccgtgacggc ggtgcgggaa
     3781 gcgctcgccc tcccgaccag cgtgcaaacc gtgcgcttcg tgtgcttcgg cgatcgggac
     3841 tggaacgtgt acctcgagct gctggggcac cggcgcgatt gctgaatgcg gtccggccgg
     3901 accactttcg ttcgtcatgg gcgcatttcc gcgcgagcgg cgcgggcggg cgccgtttgt
     3961 ccgggttgcg ttgaccggaa ctt
//
LOCUS       DBNGZW010000237         2572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_225632, whole genome shotgun sequence.
ACCESSION   DBNGZW010000237 DBNGZW010000000
VERSION     DBNGZW010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_225632"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="AC16JC_05650"
     CDS             complement(<1..661)
                     /locus_tag="AC16JC_05650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164216.1"
                     /translation="MRFTFPNRLLLASGLLFLIGAPLLSTKRAVAHTTDKCPDPSAAT
                     SAFALDYQGLETRSPVSSAFVLPGQTLEVAVEPSTSAESVAVHASAGLVSQAGTSEWR
                     WHAPAAPGSYTLRVVGSCQADSAVVHAFVMVPASDVHAGYLNGFRIGSYPRPPLRRQA
                     TYQPPAGFIEVTPQNQDVFITPHLQLKQFLTKQPGGYPKYVVLDPRLLLKLELIFKEV
                     HD"
     gene            complement(801..873)
                     /locus_tag="AC16JC_05655"
     tRNA            complement(801..873)
                     /locus_tag="AC16JC_05655"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(838..840),aa:Phe,seq:gaa)
     gene            complement(961..>2572)
                     /locus_tag="AC16JC_05660"
     CDS             complement(961..>2572)
                     /locus_tag="AC16JC_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HIR5164217.1"
                     /translation="RCLSLVLAAVLGGGTLVAQAPAKRRVAVLDFDYATVHQYVYDLF
                     GSDQDIGKGIADMLVTNLVRNGTYSVIERKQLDRVLQEQNFQQSGRADPSSAVQLAKI
                     LGVDAIIIGSITQFGRDDKKLGVGGIGARVGGIGLGGLGKKSAKAVVQIDARIVSTTT
                     AEILGVASGHGESKRSSVSMVGGLAIGGTGVGGGLDMGSTNFANTIIGEATRAAVDSL
                     TGQLVATSTTIPENVVQIQALVADVSGGEVTINVGTGAGVRVGGEYNVVRPGREIRDP
                     ATGRVLRRVTTAVGKLKITQADEGSATGTLSGGPARVGDCVGSCPANTGGGGAPPAVE
                     PLNQANAPAPPSGGGNLPALYSRPVTGPFTWGMYGFKGTEHFRYNVQQRDGSENKTGS
                     YTFDARPAGGGRFTLSVAGTLGSDSYSSTVTLGPNEGVPMMQLVALGPVGLALFNPVW
                     MMLLGGHQWEIGNGWQQTQNGKTTSFRVEAQCAQAGVSGARGVWRENQQVLMDMCVSP
                     NVGLPLAVTFNGDDGSSSTAMQLVEFRP"
BASE COUNT          432 a          903 c          811 g          426 t
ORIGIN      
        1 cgtcgtgcac ttctttgaag atcaattcga gcttcagcag caaccgagga tcgagcacga
       61 cgtacttggg atatcccccc ggctgtttcg tcaggaactg cttcagctgc agatggggcg
      121 tgatgaacac gtcctgattc tgcggcgtga cttcgatgaa gccggccggc ggctgatagg
      181 tcgcctgtcg gcggagcggt ggccgaggat agctgccgat gcggaagccg ttgaggtagc
      241 cggcgtgcac gtcgctggcg gggaccatga cgaacgcgtg tacgaccgct gaatccgcct
      301 ggcagctgcc cacgacccgc agcgtgtagc tccccggtgc ggcgggagcg tgccagcgcc
      361 actccgatgt ccccgcctgc gagacgaggc cggcggacgc atgcaccgcg acgctctcgg
      421 cggaggtcga gggttccacc gcaacctcga gcgtctgtcc ggggagcacg aatgcggagc
      481 tgacgggaga gcgggtctcc aggccttgat agtcgagcgc gaacgccgaa gtcgcggcgc
      541 tcgggtccgg gcacttatcc gtcgtgtggg cgacagcccg tttggtgctc aacaacggag
      601 ccccgatgag gaacaggagt ccactggcga ggagcagccg attcggaaac gtgaaacgca
      661 tagggcggag aaactaatgt ggccccttgg tcgcaccaat gacagaaacc ttcatcttct
      721 cgccccgaac cccgcggcac atcacaaatg aagaacgggt ctcttgtgca gagacccgtt
      781 gcagggaacc ctcacgccag tggccagggg cagaatcgaa ctgccgacac gcggattttc
      841 agtccgctgc tctaccaact gagctacctg gccgacagaa tggaaggggg attatagaac
      901 gaaggcgtcc tgccgccaac cgccccctcg caccacagtg agggggcggc gcagacctga
      961 ctacgggcgg aactccacca actgcatcgc cgtcgagctc gacccgtcgt cgccgttgaa
     1021 ggtgaccgcc agcggtaggc cgacgttcgg gctcacgcac atatccatca gcacctgctg
     1081 attctcgcgc cacaccccgc gcgcgccgct cacgcccgcc tgcgcacact gtgcctccac
     1141 tctgaacgag gtggtcttgc cgttctgggt ctgttgccac ccgttcccga tctcccactg
     1201 atgtccgccg agcaacatca tccacaccgg gttgaagagc gcgagcccca ccggccccag
     1261 cgccaccaac tgcatcatcg gcacgccttc attcgggccc agcgtgaccg tcgaggagta
     1321 gctgtcgctg ccgagcgtgc ccgcaaccga gagcgtgaag cggccgccgc ccgccggccg
     1381 cgcgtcgaag gtgtagctcc ccgtcttgtt ctcgctgccg tcgcgctgct gcacgttgta
     1441 gcggaagtgc tcggtcccct tgaacccgta catcccccag gtgaacggtc cggtcaccgg
     1501 cctgctgtag agtgcgggca gattcccgcc ccccgacggg ggggccggtg cgttcgcctg
     1561 attcagcggc tcgaccgcgg gcggtgcgcc gccacccccg gtattggccg ggcagctccc
     1621 cacgcagtct ccgacccggg cggggccgcc gctgagtgtg cccgtcgccg atccctcgtc
     1681 cgcctgggtg atcttgagct tcccgaccgc cgtcgtgaca cgccgcaaca cccgccccgt
     1741 ggccgggtcg cggatctcac ggccgggacg gacgacgttg tattccccac ccacgcggac
     1801 ccccgcgccg gtgccgacgt tgatcgtcac ttcgccgccg gacacgtccg ccaccagcgc
     1861 ctggatctgg acgacgttct cggggatagt ggtagaggtc gccacgagct ggcccgtcag
     1921 gctgtccacc gctgcgcggg tcgcttcacc gatgatggta ttggcgaagt tcgtgctgcc
     1981 catgtcgagc ccgccgccga cccccgtccc gccgatcgcc aatccgccga ccatggacac
     2041 actggagcgc ttggattcgc cgtgacccga cgcaacaccc aggatctcgg ccgtcgtcgt
     2101 gctcacgatg cgcgcgtcga tttgcaccac cgccttcgcg gatttcttgc cgagcccgcc
     2161 aagtccgatg ccgccgaccc gcgccccgat accgcccacg ccgagcttct tgtcgtcacg
     2221 gccgaattgc gtgatgctgc cgatgatgat cgcatcgacg ccgaggatct tcgccagctg
     2281 cacggccgag gacggatcgg cccgcccgga ctgctggaag ttctgctcct gcagcacccg
     2341 gtcgagctgc ttgcgctcga tcaccgagta ggtgccgttg cgcacgagat tcgtgaccag
     2401 catgtcggcg atccccttgc cgatgtcctg gtcgctgccg aacaggtcgt agacgtactg
     2461 gtgcacggtg gcgtaatcga aatccaacac agccacccgc cgcttggcgg gagcctgcgc
     2521 gacgagtgtc ccgccaccca gcacggcggc caggacgagc gacagacagc gc
//
LOCUS       DBNGZW010000238         5103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1452931, whole genome shotgun sequence.
ACCESSION   DBNGZW010000238 DBNGZW010000000
VERSION     DBNGZW010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5103
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1452931"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="AC16JC_05665"
     CDS             complement(<1..678)
                     /locus_tag="AC16JC_05665"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR5164218.1"
                     /translation="MEPVSVRSGPITLLLAVFFGGAGALTGQARWTVEPKTSLAWWQV
                     DPHYNHLWATTCPADHSWQPGEGRDPGQYVDYKTRPDLRDNGGHETRVPLFPRLQVNS
                     VCRQAVSGTILIEDTLHWKGVRGEVTLNPDSLFSGLAFRDDFARSHVLNTVDYPRIRF
                     VIDSLVDVKPGPGDTLNAIAVGSFSLHGVTSPMQAAVQAWRDPAGFRVRAAFDFDAQD
                     LTRKYGMS"
     gene            880..1263
                     /locus_tag="AC16JC_05670"
     CDS             880..1263
                     /locus_tag="AC16JC_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5164219.1"
                     /translation="MLLALSAAALSVSVLHAQASGVSTDPGVIKKGKSLWNTKGCMGC
                     HTIGKGRAAGPDLMGVLDRRSLDWVQRWLHDPPAMQQTDSTAKALVAEFNNTKMPNLQ
                     LSDEEVTALIAYVADQGSKMAAKSR"
     gene            1323..2675
                     /locus_tag="AC16JC_05675"
     CDS             1323..2675
                     /locus_tag="AC16JC_05675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164220.1"
                     /translation="MSRALLLAVMAATGASPAVAAGSSNAVPLFARQTGLTCAQCHVL
                     YGAPVPSFTFTGKKFRMNGYRMPWVSQKIEAGEPGAINGRRLDINLVPYLSFRYQSVF
                     AQQSKAPGAVDPGPISSNPTSRLAIFSGGAFGDNFGLWTEFYLTPPNDNPGGEWGIGN
                     FTYDEYDLRYVKTVGDNNTIGLGFSNQGVSEISGFGPWSPGGSLITTGGVAGWAHPNR
                     GQLFGYGWLRDQLFFSLGAQPGMDNLSWSKSSIIGNVAFAPFHEDQKEAWVNVEFSFG
                     NDNIPLLTNRSSVTNNTTWNTTDAVGGVSATHGGVPFSARDIKTTRRLTGELLYGFVD
                     HGPHSINLGASYNLNKDNYQNTATAEVNSVFGRIRYVYERTWGLDVQVGKQVSYKYTD
                     ATALAHTIPNKLAYNMYGTFQPAMNVLIALNYGRSQTLALDTPALTGWNWTLNIDYLF
                     "
     gene            2779..3294
                     /locus_tag="AC16JC_05680"
     CDS             2779..3294
                     /locus_tag="AC16JC_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164221.1"
                     /translation="MNDVKLVRIALAAVVAAGCFHPYGKVSEFESTKIPGSRFHTLTV
                     ISGETEHSSILIAARVRDSLQASTIVNVVNRSGRWVDEAGALREICPDSSQAGALTDS
                     SRAAIANDSSGILAAMAPVDGVVIVWWNRISLRDCRTQTTAYDATGAAQIGIDELTRQ
                     LLRYLKADRRP"
     gene            complement(3312..3506)
                     /gene="infA"
                     /locus_tag="AC16JC_05685"
     CDS             complement(3312..3506)
                     /gene="infA"
                     /locus_tag="AC16JC_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018197009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HIR5164222.1"
                     /translation="MMGTVTEVLPNTTFRVKLENGHEVLAYVSGKMRKNYIRILQGDR
                     VAVDLSPYDLTRGRITYRYK"
     gene            3689..3973
                     /locus_tag="AC16JC_05690"
     CDS             3689..3973
                     /locus_tag="AC16JC_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164223.1"
                     /translation="MDARELQRFADIQVDLVRPEQQGFILSGQGSDQAEYRLEVHFDM
                     PLDGRTRTVLGELLAQSEVSIWRGPAAAAMVAPPRRRTTRRHPRRRIPAD"
     gene            4023..>5103
                     /locus_tag="AC16JC_05695"
     CDS             4023..>5103
                     /locus_tag="AC16JC_05695"
                     /inference="COORDINATES: protein motif:HMM:NF015724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5164224.1"
                     /translation="MTTSWVRALVTAVACVAVAACAAGGARTASLSPEGVAALEAQRA
                     QHPTDPDLNLRVAKAYQAANRFADARAALATVLLQQPGNRVAQAYLGFTYEGLEQFDS
                     ARAVYGTLLAGRPSAEVSRLLNGRLAVLARKELHATARVALARESELSRTPPDPSTVA
                     VFPFRYTGQDSSLRPLERGLAALVVSDLSHVRSLRLVERERLQALLDEMQLSTSGRVD
                     PATGARSGHLVGAGQVVQGQFQEVPTAGVRLDAAMVRAADAGVAATGSGRDQLSLLFD
                     VEKSVVFQLLGKMGVPLSPAESLAISERPTRDLQAFLLYSRGLEQEDRGDFSAASASF
                     RAAAQRDPSFQAAAQQAQSSDAAQSA"
BASE COUNT          861 a         1642 c         1736 g          864 t
ORIGIN      
        1 ggacatgccg tatttcctgg tcaggtcctg cgcgtcgaaa tcgaacgcgg cgcgcacgcg
       61 gaaccccgcc ggatcccgcc aggcctgaac ggccgcctgc atcggcgacg tgacgccgtg
      121 cagcgagaac gaccctacgg cgatcgcgtt cagggtgtcg ccgggtccgg gcttcacgtc
      181 gaccaggctg tcgatcacga agcggattct gggatagtcc acggtattga gcacatggga
      241 tcgggcgaag tcgtcgcgga aggccaggcc cgagaagagc gagtcggggt tgagggtgac
      301 ctcgccacgg acgcctttcc agtgcagcgt atcctcgatc agaatcgtcc cgctgaccgc
      361 ctggcggcac acactgttga cctgcaggcg agggaagagc gggacgcggg tctcgtggcc
      421 gccgttgtcc cgcagatcgg ggcgcgtctt gtagtccaca tactgaccgg gatctcggcc
      481 ctctccgggc tgccaggaat ggtccgccgg gcacgtcgtg gcccagaggt gattgtagtg
      541 ggggtcgacc tgccaccagg cgaggctggt cttgggctcc actgtccagc gagcctggcc
      601 ggtcagggcg cctgcgcctc caaagaagac ggccagcagc aaggtgatag ggccgctgcg
      661 gacggaaacg ggctccatta gagcctcctt gggaaatctt tcacaagaca gaccccgacc
      721 gtcccaaacg tggcggctgc ggcgggaggt cgcaagcagg cccccgggct ggtgttgcct
      781 tgacagggtc gagattgaag cgtagtagat gatttcgcag cttttcggct tcattaccac
      841 ttggacggag ggggaatggc gaatcggtgt ggccgagcca tgctgttggc gctgtccgct
      901 gccgcgttgt cggtgagcgt cctacatgcg caggcatccg gggtttctac ggacccagga
      961 gtgatcaaga aggggaagtc cctgtggaac accaagggat gtatgggctg tcacacgatc
     1021 ggcaaaggtc gagccgccgg accggatctg atgggcgtgc tggaccggcg cagcctggac
     1081 tgggtgcagc gctggctaca cgatcccccc gccatgcagc agaccgactc gaccgcgaag
     1141 gcgctggtcg cggaattcaa caacacgaag atgcctaacc ttcaactcag tgatgaggag
     1201 gtgactgccc tgatcgctta cgtcgccgat caaggttcga agatggcggc gaagtcacgg
     1261 tgaatggagt gcgcatttga ccatcacaca cgtgtctggc aactcttgag ggagggaaca
     1321 tcatgagccg cgctttgctg ctcgcagtga tggccgcaac gggggcgtcc ccggcggtcg
     1381 cggctggtag ctcgaatgcg gttccactgt tcgcccggca gaccgggttg acatgcgccc
     1441 agtgtcacgt gctgtacggc gcgccggtcc ccagcttcac gtttacgggc aagaagttcc
     1501 gcatgaacgg ctatcggatg ccgtgggtca gtcagaagat cgaggccggg gaaccggggg
     1561 cgatcaatgg ccgccgcctg gacatcaacc tggttcccta tctgagcttc cggtatcaga
     1621 gcgtattcgc ccagcagtcg aaggctcccg gtgcggtgga tcccggtccg atcagctcga
     1681 acccgacgtc gcggctcgcc atcttctcgg gcggcgcgtt cggcgacaac ttcgggctgt
     1741 ggacggagtt ctatctcacg ccgccgaacg ataatccggg cggtgagtgg ggaatcggga
     1801 acttcaccta cgacgagtac gatctgcgct acgtcaagac cgtgggcgac aacaacacga
     1861 tcggtctggg cttctcgaat cagggtgtct cggaaatctc gggcttcggc ccgtggtcgc
     1921 ctggcggctc gctgatcacc accggtggcg tcgccggctg ggcgcatccg aaccgcggtc
     1981 agctgttcgg ctatgggtgg ttgcgtgatc agctgttctt cagcctcggt gcgcaaccgg
     2041 ggatggacaa tctcagctgg tcgaagagca gcatcatcgg gaacgtcgcc ttcgcgccgt
     2101 tccacgagga ccagaaagag gcgtgggtga acgtcgagtt cagcttcggg aacgacaaca
     2161 ttccgctgct caccaaccgc agcagcgtga ccaacaacac cacgtggaat acgaccgatg
     2221 ccgtgggcgg cgtgtcggcc acgcacggtg gcgtaccgtt ctcggcgcgg gacatcaaga
     2281 ccacgaggcg actcaccggc gagctgttgt acggcttcgt cgatcacggg ccgcacagca
     2341 tcaacctggg cgccagctac aacctcaaca aggacaatta ccagaacacg gcgaccgccg
     2401 aggtcaattc ggtgttcggc cggatccgct acgtgtatga gcgcacgtgg gggctggacg
     2461 tccaggtcgg caagcaggtc agctacaagt acaccgatgc aacggcgctc gcccatacga
     2521 tcccgaacaa gctggcgtac aacatgtacg ggacgttcca gccggcgatg aacgtgctga
     2581 tcgcgctgaa ctacgggcgc tcgcagacgc tggcgctcga cactccggcg ctcacggggt
     2641 ggaactggac gctgaacatc gactatctgt tctagtcgca acgcccgcag tccggggcac
     2701 ggagcacgac gcgccgcacg gcctggagcc gggcggcgcg tcttgctgct ccctccgggg
     2761 gcggcgatac ttcagggcat gaacgacgtg aagctggtcc gcatcgccct cgccgcggtg
     2821 gtcgccgccg ggtgctttca tccgtacggc aaggtgagcg aattcgagag caccaagatt
     2881 ccgggatcgc gcttccacac gctgacggtc atctccgggg aaaccgagca ttcctcgatt
     2941 ctcatcgcgg cgcgggtacg cgattccctg caagcgtcga cgatcgtcaa cgtggtcaac
     3001 cgttcgggcc gatgggtgga cgaggcgggg gcgctgcggg agatctgtcc ggactcctcc
     3061 caggcgggcg cgctgacgga ttcgagccgg gcggcgatcg cgaacgactc ctccgggatt
     3121 ctggcggcga tggccccggt ggacggcgtc gtcatcgtct ggtggaaccg catctcgctc
     3181 cgggattgcc gcacgcagac caccgcctat gacgccaccg gggcggccca gatcgggatt
     3241 gacgagctga cacggcagct gctgcgctac ctgaaggccg acagacggcc ctagcacccg
     3301 gcggccggcg tctacttgta ccggtatgtg atgcggccgc gggtgaggtc gtacggcgac
     3361 aggtcgaccg cgacgcggtc tccctggagg atacggatgt agtttttccg catcttgccg
     3421 ctcacgtagg ccagcacttc gtggccgttc tcgagcttca cgcggaaggt ggtattgggg
     3481 agcacttccg tcaccgtccc catcatctga atcgcgtcgc cggccacgga ccgcaacggc
     3541 tcctttcctt gtggttccgg cgcgggccgg taatcccgcg ccacctttca gctttccagt
     3601 cgtgcgaccg cccccaatct agcgtcgact cgcaaccttg acactccccg ctccacgcgt
     3661 atctcttgcc cgaacaggag gttgcggcat ggacgctcgg gaactgcagc gcttcgccga
     3721 catccaggtc gatctcgtgc gtccggaaca acaggggttt atcctctccg ggcaagggag
     3781 cgaccaggcc gagtaccggc tggaagtgca cttcgacatg ccgctggatg gccggacgcg
     3841 caccgtcctg ggggagttgt tggcccaatc cgaggtttcc atctggcggg gacccgcggc
     3901 cgcggcgatg gtggctcccc cgcgccgccg cacgacccgt cgacacccac gccggcggat
     3961 ccccgccgat tgaccacccg gagcacacgc ggcacgtggt ttgcttcagc caaggggagc
     4021 cgatgacgac ctcctgggtc cgcgcactgg tcacggccgt cgcgtgcgtg gccgttgccg
     4081 cgtgcgctgc cggtggcgct cgaacggcgt ccctcagccc tgaaggggtg gcggcgctgg
     4141 aggcccagcg cgcgcagcat ccaacagatc ccgacctgaa tctgcgggtc gccaaggcat
     4201 accaggcggc gaaccgtttc gccgatgcgc gcgccgcgtt ggccacggtc ctgttgcagc
     4261 aacccgggaa tcgggtcgcc caggcgtacc tcgggttcac gtacgaggga ctggagcagt
     4321 tcgactcggc gcgcgcggtc tatggcacgt tgctggcggg ccggccgagc gccgaggtga
     4381 gccgcctgct caacgggcgt ctcgcggtgc tcgcccgcaa ggagctgcac gccactgcgc
     4441 gggtggccct ggcgcgtgaa tccgagctgt cccgcacgcc ccccgatccg agcacggtcg
     4501 cggtgttccc attccggtac acggggcagg actcgtcgct ccgcccgctg gagcgtggcc
     4561 tggccgcgct ggtcgtcagc gatctctctc acgtgcgcag tctgcggctg gtggagcgcg
     4621 agcggctgca ggcgctgctc gacgaaatgc agctctccac gtcgggtcgc gtcgatcccg
     4681 cgacgggcgc ccgctccggg cacctggtgg gcgccgggca ggtcgtgcag ggacagtttc
     4741 aggaggtgcc cacggccggg gttcgcctcg acgccgcgat ggtgcgggcc gccgatgcgg
     4801 gcgtcgcagc gaccggctcg ggccgggacc agctcagcct gctgttcgat gtcgagaagt
     4861 cggtggtatt ccagctactc ggcaagatgg gcgttccgct cagccccgcc gagagcctgg
     4921 ccatcagcga gcgccccacg cgtgacctcc aggccttcct gctctacagc cgcgggctcg
     4981 agcaggagga ccggggcgac ttcagtgcgg cgagcgccag tttccgtgcg gcggcacagc
     5041 gcgaccccag cttccaggcg gcggcacagc aggcgcaatc gagcgacgcc gcacagtcgg
     5101 cag
//
LOCUS       DBNGZW010000239         5699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_424409, whole genome shotgun sequence.
ACCESSION   DBNGZW010000239 DBNGZW010000000
VERSION     DBNGZW010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5699
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_424409"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1162
                     /locus_tag="AC16JC_05700"
     CDS             137..1162
                     /locus_tag="AC16JC_05700"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR5164225.1"
                     /translation="MLRLPSFTYRQPKTLRDALAMKADAGPDGSYVAGGTDLYPNMKR
                     RQQEPRTVISLMGVPELGAVRRSGGQAIGGLEIGACITLSELTASPPVRLSAHHAIAE
                     AAELVSTPLLRNMGTLGGNLCLDTRCNYYDQSYEWRKAIDFCMKKDGAICWVAPSSPR
                     CWAVSSSDVAPVMVALGAEYVLLGPGGERVVPAARFYNNDGINYLTKQPDEILSAVHL
                     PAADGWDAVYHKLRRRGSFDFPVLGVAVWVKWEGGRGKGEGARVGDARIVLGGVASYP
                     QEVVEAGAALKGSALTDDAIAAAAEAAFQPSKPMDNTDFDLSWRKQMTRVYVTRALAD
                     LRTRRTG"
     gene            1252..2445
                     /locus_tag="AC16JC_05705"
     CDS             1252..2445
                     /locus_tag="AC16JC_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAF repeat-containing protein"
                     /protein_id="HIR5164226.1"
                     /translation="MRSVRQVSMVTVVLLVTACETPQTPTSSLPAVQSALLAVPDPSV
                     AYTAIDIGTLGGTFSGVAAINNRGEVAGSSTTSSGDLHGYFWSSGVMTDLGTLGGSLT
                     IPSAMNDLGQVVGFSMTANIESHCFMWTQGSMTDLGGRDCHPRRINNAGQVVGLTQTA
                     TGEYHAFLWADGAITDLGTLGGPFSEATGINDAGQVVGNSTTASGESHAFSWSHGVMT
                     DLGTLGGPSSAAAALNPINSQGQIVGASQVATGERHAFLWSNGVMTDLGTLGGAYGEA
                     VAINDAGQVAGASYTASEEDHSFVWSNGVMTDLGTLGGMYTQANALNNSGQVVGGSAL
                     AAGPGHAFLWVNGVMVDLGTLGGSFSSGETIDDAGQIVGISATPGDAQIHATLWRRGP
                     AQSHR"
     gene            complement(2450..4489)
                     /locus_tag="AC16JC_05710"
     CDS             complement(2450..4489)
                     /locus_tag="AC16JC_05710"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012680440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004563 - beta-N-acetylhexosaminidase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HIR5164227.1"
                     /translation="MRRPPFGLLVMSCLVAPLAAQQSVTLDLMPVPRSLALGEGRLRL
                     DSSFTVAFAPESDARLARAVRRAMERLARRTALTLSTIPVSDSTGGLVIAALAAGQPV
                     QTETEDESYTLHITPERATLGAATVVGVLRGLETLLQLVTADSVGFYLPAVHIEDAPR
                     FPWRGLLVDAGRHFEPVEVIERTLDGMAAVKLNVLHWHLSEDQGFRVQSLRYPLLQGK
                     GSDGLFYTQAQIREVVAYARDRGIRVVPEFDMPGHTSSWSLGYPRYASGPGPYAIQRT
                     WGVFDPTFDPTRESVYVFLDRFIGEMAALFPDPYWHIGGDEVNGVEWDANPRIRAFKR
                     LHNFAADAALQAYFNRRLSRILTAHGKQMVGWDEILNPDLPASAVVQSWRGVEYLPTT
                     VRAGHRAILSAPYYLDQIFTAQDHYRDPLPDSLGLAPEEAARVLGGEACMWGEHVGPE
                     TIESRIWPRLAAVAEKLWSQASVNDVADMYRRLAVTSLRLEELGLSHTLHTERMVRRA
                     IGNGADFQALLALLEVSQPVTFGQRHRLQVGMTQQTPLTRLVDAARPDPPIRWNSLAL
                     VQRFLADSGHTPAIADTLRATFTAWRALPAAVHAAAVRAPLARDGVPAAEALGRTADV
                     GLAALDRVQGRTAVTAGWQDSARAELDSASAPQGLLRLVVVDAVRRLVETAPFGK"
     gene            4568..>5699
                     /locus_tag="AC16JC_05715"
     CDS             4568..>5699
                     /locus_tag="AC16JC_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR5164228.1"
                     /translation="MHLTQADWLIVICSIGISFIPAILLARRAGSSTAEFFTSGRAAP
                     WWLIGVSMVATTFSTDTPNLVTNFVREKGVANNWQWWAFLLTGMATVFFYAKLWRRSG
                     VLTDLEFYELRYSGKPASLVRGFRAVYLGLFFNCVIMASVNLAAAKIANVLLGWPMGK
                     TLLICAVLNVAFAATSGLWGVLVTDFIQFGIAMTGSFAAAIYALKQPEVGGLAGLIAK
                     TDPKVLSLVPDFGDWGMTLSVLIIPITIQWWSVWYPGAEPGGGSYIAQRMLAARSEKD
                     AVGGTLLFNIAHYALRSWPWIIVALASILVYPQLSDIARTFPYVDPKLIGHDMAYPAM
                     LRFLPHGMLGLMVAGLLAAYVSTISTHLNWGTSYLVHDCYRRF"
BASE COUNT          917 a         1923 c         1962 g          897 t
ORIGIN      
        1 atacaaggtc caatagcgaa cgccaacgag cgcgtaatag tcctgtcatc caggtactat
       61 gccgcggcaa cacgccgtcg ggccgcgcca tcgcgcgggc tggccacgac cggggcgccg
      121 cattaaggtt tcggccatgc tgcggctgcc gtcgttcacg taccgtcaac cgaagaccct
      181 ccgggacgcg ctcgcgatga aagcggacgc gggcccggac gggtcgtacg tcgccggcgg
      241 gacggatctc taccccaata tgaagcggcg ccagcaggag ccgcgcacgg tgatctcgct
      301 catgggggtg ccggagctcg gggcggtcag gcggtcgggc ggtcaggcga tcggtgggct
      361 cgagatcggt gcatgcatca cgctcagcga actgaccgcc agtccgcctg tccgcctgtc
      421 cgcccatcat gccatcgcgg aggccgccga gctggtttcc actcccctgc tccgcaacat
      481 gggaacgctg ggcggcaatc tctgtctcga cacgcgctgc aactactacg accagtccta
      541 cgagtggcgc aaggcgatcg acttctgcat gaagaaggac ggtgcgatct gctgggtggc
      601 gccgtccagt ccgcgctgct gggcggtgag ctcgtcggac gtggcgccgg tgatggtggc
      661 gctcggcgcc gagtacgtgc tgctgggtcc ggggggcgag cgcgtcgtcc cggccgcgag
      721 gttctacaac aacgacggga tcaactacct cacgaagcag cccgacgaga tcctcagcgc
      781 cgtgcatctc cccgccgccg atggctggga cgcggtctat cacaagctgc gccggcgggg
      841 gagtttcgac ttcccggtcc tcggggtcgc cgtctgggtc aaatgggaag ggggaagagg
      901 gaagggggaa ggggcgcgcg tcggcgatgc gcggatcgtg ctgggcggcg tagcgagtta
      961 tccgcaggag gtcgtcgagg cgggggcggc gctgaagggc tcggcgctca ccgacgatgc
     1021 gatcgccgca gccgctgagg cggcgtttca gccctccaaa cccatggaca ataccgactt
     1081 cgatctctcc tggcggaaac agatgacccg ggtgtacgtc acgcgtgcgc tcgcggactt
     1141 gaggacgcgc cgcaccggct gatcgctgcc gctctgcttg acgtccaatt gacacgcacg
     1201 cggttgattc cctcccctga tcacgatcgg tccgatcacc cgggaggccc catgcgaagc
     1261 gtgcgacagg tatcgatggt cactgtggtg ctgctggtca cggcgtgcga aacgccccag
     1321 acaccgacga gttccctccc cgcggtgcaa tccgcattgc tggccgttcc cgacccgagc
     1381 gtcgcctaca cggcgatcga tatcggcacc ctgggaggga cgttcagcgg cgtcgccgcc
     1441 atcaacaacc gcggagaggt cgcgggttcc agcacgacct ccagcggtga cctgcacggg
     1501 tacttctgga gcagcggggt gatgaccgac ctcggcaccc tcggcggctc gctcaccatc
     1561 cccagcgcca tgaacgacct cggccaggtt gttgggttca gcatgaccgc caacatcgaa
     1621 tcccactgtt tcatgtggac gcaggggagc atgaccgacc tgggcgggag ggattgccac
     1681 cccaggcgca tcaacaacgc cgggcaggtc gtgggtctca cccagaccgc aaccggcgaa
     1741 tatcacgcgt ttctctgggc agacggagcg atcaccgacc tcggcacgct cggtggtcca
     1801 ttcagcgagg ccacgggcat caacgacgcc ggtcaggtcg tcggcaacag cacgacggcc
     1861 agcggcgagt cccacgcatt ctcttggtcg cacggcgtga tgaccgacct cggcaccctc
     1921 ggcgggccgt ccagcgccgc ggcggcactc aacccgatca acagccaggg ccagatcgtc
     1981 ggcgcgagcc aggtagcgac cggagaacgc catgcgttcc tgtggtcgaa cggggtgatg
     2041 accgatctcg gcacactcgg cggcgcgtac ggcgaggcgg tcgccatcaa cgacgcggga
     2101 caggtcgcgg gcgcgagcta cactgcgagc gaagaggacc actccttcgt gtggtccaac
     2161 ggcgtgatga cggacctcgg cacgctggga gggatgtata cgcaagcgaa tgccctgaac
     2221 aactccggtc aggtcgtcgg ggggagcgcg ttggccgctg gtcccggtca cgcgtttctc
     2281 tgggtgaacg gcgtgatggt cgatctcggc acgctgggcg gatcgttcag ctcgggcgag
     2341 acgatcgacg atgccggaca aatcgtcggt atcagcgcga cccctgggga tgcgcagatc
     2401 cacgcgacac tgtggcgccg cgggccggcc cagtcacacc gctgacgatc tacttgccga
     2461 acggggcggt ttccacgagc cggcggacag cgtccactac cacgaggcgc agcaacccct
     2521 gaggcgccga cgcgctgtcg agctccgctc gggccgaatc ctgccagccc gccgtcacgg
     2581 ccgtgcgtcc ctgcacgcgg tcgagcgccg ccagtcccac gtcggcagtc cgacccagcg
     2641 cctccgccgc ggggacgcca tcgcgcgcga gcggcgcgcg cactgcggcc gcgtgcacgg
     2701 cggcgggcaa cgcccgccac gccgtgaagg tcgcacgcag ggtgtcggcg atcgccggag
     2761 tatggccgct gtccgcgagg aaccgctgca ccagggcgag cgaattccac cgtatgggcg
     2821 ggtcgggacg ggccgcgtcg acgaggcggg tcaggggggt ctgctgcgtc atccccacct
     2881 gcaggcgatg acgctggccg aacgtgacgg gttgcgacac ctcgagcagc gccagcaacg
     2941 cctgaaagtc cgccccgttc ccgatcgccc gccgcaccat gcgctcggtg tgcagggtgt
     3001 gcgagaggcc cagttcctcg agacgcagac tcgtcacggc cagccggcgg tacatgtcgg
     3061 ccacgtcgtt caccgacgcc tgggaccaga gcttctccgc cacggcggcg agccggggcc
     3121 agatgcgcga ctcgatcgtc tcgggcccga cgtgctcgcc ccacatgcac gcctcgccgc
     3181 ccagcacgcg cgcggcctct tcgggggcca ggccgagcga gtccgggagc ggatcccggt
     3241 agtggtcctg agccgtgaag atctgatcga ggtagtacgg cgccgagaga atcgcacgat
     3301 ggcccgcgcg caccgtggtg gggaggtact ccacgccgcg ccatgactgc accacggcgc
     3361 tcgccgggag gtcggggttc aggatttcgt cccagccgac catctgtttg ccgtgcgccg
     3421 tgaggatgcg cgacaaccgc cggttgaagt aggcctgcag cgcggcgtcg gcggcgaaat
     3481 tgtggagccg cttgaacgcg cggatgcgcg ggttcgcgtc ccactccacg ccgttcacct
     3541 cgtccccccc gatgtgccaa taggggtccg ggaacagcgc cgccatttcc ccgatgaagc
     3601 ggtcgaggaa gacgtacacc gactcgcgag taggatcgaa ggtcgggtcg aacacgcccc
     3661 aagtccgctg gatggcgtag ggacccggtc cgctcgcgta gcgcgggtag cccaaggacc
     3721 aggaagaggt atggcccggc atgtcgaact cggggacgac gcggataccg cggtcgcggg
     3781 cgtaggcgac cacctcccgg atctgggcct gcgtgtaaaa cagcccgtcc gaccccttcc
     3841 cctgcaacag cgggtagcgc aggctctgca cgcgaaagcc ctggtcttcc gaaaggtgcc
     3901 agtgcagcac gttcagcttg acggcggcca tgccgtcgag cgtgcgctcg atcacctcga
     3961 cgggctcgaa atggcgcccg gcgtccacca gcagcccgcg ccagggaaag cgcggggcgt
     4021 cctcgatgtg gacggccggg aggtagaagc cgacgctgtc tgcagtgacg agctggagca
     4081 gcgtctcgag gccgcgcagc acgcccacca ccgtcgctgc gccgagcgtc gcgcgctcgg
     4141 gagtgatgtg cagcgtgtag gactcatcct cggtttccgt ctggaccggc tggcccgccg
     4201 cgagcgcggc gatcacgagc cctccggtcg agtcggacac ggggatggtc gagagcgtaa
     4261 gcgccgtgcg gcgcgccagg cgctccatcg cgcgcctcac ggctcgcgcg agccgggcgt
     4321 cgctctcggg cgcgaaggcg acggtgaagc tggaatcgag ccgcaggcgc ccttctccca
     4381 acgcgagcga cctggggacg ggcatcaggt cgagggtgac cgactgctgc gcggcgagcg
     4441 gggccacgag acacgacatc acgaggagac cgaagggtgg gcggcgcatg gggcgaatat
     4501 agggtccgtt gcggccgccc ggcgcgtccc tatcttagcc cggtcccttc cctggtgccg
     4561 atcgcccatg catctcacgc aggccgactg gctcatcgtc atctgctcga tcggcatctc
     4621 cttcatcccg gccatcctgc tggcccgccg ggcgggatcg agtaccgccg agtttttcac
     4681 ctcgggccgt gcggcaccgt ggtggctcat cggcgtgtcg atggtggcga ccacgttctc
     4741 gaccgacacg ccgaacctgg tcaccaactt cgtgcgcgag aaaggcgtcg ccaacaactg
     4801 gcagtggtgg gcgttcctgc tcaccggcat ggccacggtg ttcttctatg ccaagctgtg
     4861 gcgccgctct ggggtgctca ccgacctcga gttctacgag ctgcgctaca gcggcaagcc
     4921 ggcgtcgctg gtgcgcggct tccgcgccgt ctacctcggg ctgttcttca attgcgtcat
     4981 catggcgtcg gtgaacctcg cggccgccaa gatcgccaac gtgctgctcg gctggccgat
     5041 ggggaagacg ctgctcatct gcgccgtact gaatgtggcg ttcgcggcga catcgggact
     5101 gtggggcgtg ctggtcaccg acttcatcca gttcgggatc gccatgaccg gctccttcgc
     5161 cgcggcgatc tacgccctga agcagccgga ggtgggcggc cttgcggggc tcatcgcgaa
     5221 gaccgacccc aaggtcctga gcctggtccc tgatttcggc gactggggga tgacgctttc
     5281 cgtgctgatc atccccatca cgatccagtg gtggtcggtg tggtatccgg gagcggagcc
     5341 gggaggcggg agttacatcg cccagcgcat gctcgccgcc cggagcgaga aggacgccgt
     5401 gggcggcacc ctgctcttca acatcgccca ctacgcgctg cggtcgtggc cgtggatcat
     5461 cgtcgccctg gcgtcgattc tcgtctatcc gcagctctcc gacatcgcgc gcaccttccc
     5521 gtacgtggac ccgaagctca tcgggcacga catggcctac cccgcgatgc tccgcttcct
     5581 gccgcacggg atgctcggct tgatggtggc ggggctgctg gccgcgtacg tgtccacgat
     5641 ctcgacccac ctgaactggg gcacgtccta tctggtgcac gactgctacc ggcgattcc
//
LOCUS       DBNGZW010000240         4086 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_907803, whole genome shotgun sequence.
ACCESSION   DBNGZW010000240 DBNGZW010000000
VERSION     DBNGZW010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4086)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4086)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4086
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_907803"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..596)
                     /locus_tag="AC16JC_05720"
     CDS             complement(39..596)
                     /locus_tag="AC16JC_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164229.1"
                     /translation="MPYDLGRADARLKWAVTCLLLTFGLAYLFGGWMVALYAGFGPQR
                     IAATYAQPAEAPAPDTTMVVEHTMPMSEFDVADSTEVHFVDTKLLVQDTHVHVPMYGV
                     IAALLSLVVLGLGVSRRWGYALITLLFAAPWLDFAGMWLTKFVAPPFALLTAAGGWTM
                     GLGYSVVTLLALRRMWLPTKEGTAT"
     gene            complement(608..1687)
                     /locus_tag="AC16JC_05725"
     CDS             complement(608..1687)
                     /locus_tag="AC16JC_05725"
                     /inference="COORDINATES: protein motif:HMM:NF019268.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR5164230.1"
                     /translation="MRVTLGCLLACSVVRIAAAQDTTVTFEESHTVSVSGFAVGTFDW
                     DRNARANSFAGSKIALSLFRPWSDYLYLFGQLTTSLATDEVTGDPVTETEIDNLLVSW
                     SPPGATSWNFTFGRFDAPLGFERDDEPLNLIPTGSFTFELARPVKLTGALARYAVSPR
                     VTAAAYVANGWNVELDNNRGKTAGVRVDLFPAEGVAVGINALYGPEVAGTDAQQRTLF
                     AADATLQPAPRLIVGVEADYGRQLLGGGAAGTWTGVVATAFWRLQRSFGLTLRAETLH
                     DADGVATGTAQTLQSLTLSPWYFYREAQEGIFSSIEHTTFRLPAFSLRPALRIDHSDQ
                     PVFPTATGGLRDSNVRWIVELVYLF"
     gene            complement(1733..2083)
                     /locus_tag="AC16JC_05730"
     CDS             complement(1733..2083)
                     /locus_tag="AC16JC_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164231.1"
                     /translation="MRLTVRSGRRLFALAFACWVMAVPLTSFARDAALCRHRAMHQDM
                     SPRDSSGAPCWCSDMAGNATPIAAETPSLPADLVVMPAGPRSAGSIALPRAGSPPTSP
                     SYAPTPPPPNARNG"
     gene            2323..3990
                     /locus_tag="AC16JC_05735"
     CDS             2323..3990
                     /locus_tag="AC16JC_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164232.1"
                     /translation="MLAGGVLVTSLVLLSNCSAADKTGPPPVATQLAFKIDPSTSPAG
                     GVIPPVTVQARDASGKLVSGFSDSVTVTLAVNPTNGVLLGTRTVAAVGGVATFTNLSL
                     QTAGTGYRLQAASGGLTPTLSAKFDIMPGPATHLGFTAQPGTSVAGTAIAPAVKVTAL
                     DALENAATGFSGAITVAISSGTGASGAALSGITTANSSAGVATFSTLSIDKMGIGYRL
                     AASSASLSPVTSDSFDIVAGAAAQLVFITPPATTTAGTIIAPPIQVEVRDAVGNPVTG
                     FAGNVTVSITTGSGTSGATLAGTRTVLVSGGIATFSDLSINRSGTAYTIAARSSGLAG
                     ATSGPFDILPGPVAALAFSAQPQTSTAGATLPAVAVTAQDASGNIVPSFADPVTLSIS
                     ANPGNGTLHGSTTQAAVNGVATFADLSIDSVGIGYRLQASAGALSATSQAFSIRPGNA
                     SRLVFTVQPPGTTVAGAAIAPAVQVSQLDSLGNIVTAFTGAVTVALTAGKGTAGATLS
                     GTATVNAVAGVASFTTLSIDSVGTGYTLTATTDGLPDTVSAAFSIIP"
BASE COUNT          643 a         1392 c         1481 g          570 t
ORIGIN      
        1 gtgcagcagg gcgacgagcg cgcccacgag cagcttgttc atgtcgcggt tccttccttg
       61 gtcggcagcc acatacggcg cagcgcgagc agcgtgacca cggagtaacc cagtcccatg
      121 gtccaccccc cggccgccgt gagcagggcg aatggcgggg cgacgaactt ggtgagccac
      181 atccccgcga agtcgagcca gggcgcggcg aagagcaggg tgatcagggc gtagccccag
      241 cgccgggaga cgccaaggcc gagcacgacg agcgacagca gtgcggcgat cacgccgtac
      301 atcggcacgt gcacgtgggt gtcctgcacg agcagcttcg tatccacgaa gtggacctcc
      361 gtcgagtccg ccacgtcgaa ctcgctcatc ggcatcgtat gctcgacgac catcgtcgtg
      421 tccggggccg gggcctcggc cggctgcgcg tacgtggcgg cgatgcgctg cggaccgaag
      481 ccggcataca gcgcgaccat ccacccgccg aacagatagg cgaggccgaa ggtgagcagc
      541 aggcaggtga cggcccactt gaggcgcgcg tccgcgcggc cgaggtcgta gggcatgtcg
      601 actccgctca gaagaggtac accagctcca cgatccagcg gacgttcgag tcgcgcaggc
      661 ccccggtcgc agtcgggaag acgggctggt cggagtgatc gatccgcaac gcgggccgca
      721 gggagaacgc cggcagccgg aacgtcgtgt gctcgatact cgagaagatc ccctcctgtg
      781 cctcgcgata gaagtaccag ggagagaggg tgagcgactg gagcgtctgc gccgtgccgg
      841 tggccacacc gtcggcgtcg tggagcgtct ccgcccgcag cgtgagtccg aacgagcgct
      901 gcagccgcca gaacgcggtg gcgactacgc ccgtccaggt gccggcggcg ccgccgccca
      961 gcagctgccg gccgtagtcg gcttcgacgc ccacgatgag ccgcggagcg ggctggagcg
     1021 tcgcatcggc ggcgaacagg gtgcgttgct gggcgtcggt gccggccact tcgggaccgt
     1081 acagggcatt gatcccgacg gccacgccct cggcgggaaa caggtccacg cgcacgccgg
     1141 cggtcttccc gcggttgttg tccagctcga cgttccagcc gttggccacg taggcggccg
     1201 cggtgacccg cggggacacg gcgtagcggg cgagggcgcc ggtgagcttg acgggccgcg
     1261 ccagctcgaa ggtgaagctg ccggtgggaa tcagattcaa cggctcgtca tcacgctcga
     1321 agccgagcgg ggcgtcgaat cggccgaagg tgaagttcca cgatgtcgcg ccggggggcg
     1381 accagctgac gagcaggttg tcgatctcgg tctccgtcac gggatcgccc gttacctcgt
     1441 cggtggcgag gctcgtggtc agctggccga agagatacag gtagtccgac caggggcgga
     1501 acaggctgag cgcgatcttg gagcccgcga aactgttggc gcgggcgttg cgatcccaat
     1561 cgaacgtccc gacagcgaag ccgctgacgc tgacggtatg ggactcttcg aacgtgaccg
     1621 tggtgtcctg cgcggcagcg atgcggacga cgctgcacgc caggaggcac ccgagcgtga
     1681 cacgcatggg tctttcctcc aactacgcga attgtgagcg gacgacggcg attcagccgt
     1741 tgcgcgcgtt cggaggtgga ggggtcggag cgtagctggg ggaagtgggc ggcgatccgg
     1801 cgcgcggcag tgcgatcgac cccgcggacc gaggaccggc cggcatcacg acgaggtcgg
     1861 ccggcaggga gggcgtctcc gccgcaatgg gcgtcgcatt cccggccatg tcgctgcacc
     1921 agcagggcgc accggaggag tcccggggcg acatgtcctg gtgcatcgcg cgatggcggc
     1981 acagggccgc gtcccgggcg aacgacgtca gcggcaccgc catgacccag caggcgaaag
     2041 cgagggcgaa caggcggcga ccggaccgca cggtgagtcg catcgaggat ggatgttagg
     2101 aaggcgaggc gtatgcggca acccggcggt gtcaaattgt agtgcggatt gtggcgcgcc
     2161 ccccacacta cggagggaga gggcggcggg cgcacgggag ttcgcgcggc tgcactcggg
     2221 cacaaaacct gccgtccgtc ttatgccttg ctgcgtcatc cgtccgcgcg ccatcggctc
     2281 gctgccgccc tccgagtccc gccccggctc ggcctttcgg ccgtgctcgc cggcggggtg
     2341 ctcgtcacgt ctctcgtgct gttgtccaac tgcagcgcgg ccgacaagac cgggccgccg
     2401 ccggtcgcta cccagctggc cttcaagatc gatccctcca cctcgccggc cgggggggtc
     2461 atcccgccgg tgacggtcca ggcccgggat gcttccggga agctcgtcag cggcttctcc
     2521 gacagcgtga ccgtgacgct cgccgtgaac ccgacgaacg gcgtcctgct cggcaccagg
     2581 accgtggcgg cggtgggcgg cgtggccacg ttcaccaatc tgagtctcca gacggccggc
     2641 acgggctacc ggctgcaggc cgcctccggc ggcctgacgc cgacgctcag tgcgaagttc
     2701 gacatcatgc cgggaccggc gacccatctc gggttcacgg cccagccggg gacgtcggtg
     2761 gccgggacgg cgatcgctcc cgccgtgaaa gtgacggcgc tggatgccct ggagaatgcg
     2821 gcgacggggt tctcgggcgc catcaccgtg gcgatcagca gcggcaccgg cgcgagcggg
     2881 gccgcgcttt cggggatcac gacggcaaac tcgtccgcgg gcgtggcgac cttctccacg
     2941 ctcagcatcg acaagatggg aatcgggtac cggctcgccg cctcctccgc gagcctgtcc
     3001 ccggtgacga gcgattcctt cgacatcgtc gccggcgccg cggcccagct cgtcttcatc
     3061 acgccgccgg cgacgaccac ggccgggacg atcatcgcgc cgccgatcca ggtcgaggtg
     3121 cgcgacgccg tggggaaccc ggtcacgggg ttcgccggca acgtcacggt gtcgatcacg
     3181 acggggagcg gtacgagcgg ggccacgctg gccgggacga gaacggtgct ggtcagcggc
     3241 gggatcgcca ccttctccga tctgagcatc aaccgcagcg ggacggcgta cacgatcgcg
     3301 gcgcggtcga gcggcctggc cggcgccacc agcgggccgt tcgacattct ccccggtccg
     3361 gtggcggcgc tcgcgttctc ggcgcagccg cagacctcga cggcgggcgc gaccctgccc
     3421 gcggtcgcgg tcacggccca ggacgcgtcc ggaaacatcg tgccgtcttt tgccgatccg
     3481 gtgacgctgt ccatcagcgc caaccccggc aatggaacat tgcacggcag cacgacgcag
     3541 gcggccgtca acggtgtcgc gaccttcgcc gacctgagca tcgacagcgt cgggatcgga
     3601 taccggctcc aggcctccgc gggtgccctg agcgccacca gccaggcctt cagtatcaga
     3661 cccggcaacg cgtcccggct ggttttcacc gtgcagccgc ccggcacgac cgtggcgggc
     3721 gcggcgatcg cgccggccgt gcaggtgagc cagctggatt cactggggaa catcgtcacg
     3781 gccttcaccg gcgcggtgac ggtggcgctc acggccggga agggtaccgc cggcgcgacg
     3841 ctctccggca cggcgacggt gaacgcggtc gccggcgtgg cgtccttcac gacgctcagc
     3901 atcgactcgg tcggcaccgg ctataccctg acggcgacga cggacggtct ccccgacacc
     3961 gtcagcgccg ccttcagcat catcccctga gcggccgccc ggctcgagta cacggtgcag
     4021 ccgacagcgg tcacggcggg cgaggtgttc gctccgccga tcgcggtcac ggcgcgggat
     4081 gcgctc
//
LOCUS       DBNGZW010000241         3114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1182378, whole genome shotgun sequence.
ACCESSION   DBNGZW010000241 DBNGZW010000000
VERSION     DBNGZW010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3114
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1182378"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="AC16JC_05740"
     CDS             complement(<1..946)
                     /locus_tag="AC16JC_05740"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5164233.1"
                     /translation="MGILRGWQLRLRALFRRVTTERDLDEEIRLHLELEAEKHRALGL
                     APEAARRAALVSFGGVEVVKEAHRDGRGVRWLEDAIADARYALRTLRHNPALSGTAIL
                     TLALGIGANTAIFSAVNAVILRPLPFPHSEHLVMLSEDNPEKGWRRQVAAPANYLDWR
                     ERVHAFEDVAAYAPGGGTTLSGVDTPRRVRERSVTGNYFAILQVRPEAGRVFADAETW
                     SGGTPVAVVSHRLARDLFGGADRAVGRTVSLDGTLTEIVGVLPAEFTFAADSFDLWRP
                     MRWDPQLRSQDFFRRAHWLRVVARLKPGVTPRAADAEFQ"
     gene            complement(946..1278)
                     /locus_tag="AC16JC_05745"
     CDS             complement(946..1278)
                     /locus_tag="AC16JC_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR5164234.1"
                     /translation="MAPPSRDVLRGTLDLLILKTLTLEPMHGWGISQRIQQISRGVLE
                     VNQGSLYPALQRLELRGWIDSEWRTSDHNRRAKYYRLTASGRRALGAETDSWRRYVAA
                     VDWILETP"
     gene            1404..2162
                     /locus_tag="AC16JC_05750"
     CDS             1404..2162
                     /locus_tag="AC16JC_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164235.1"
                     /translation="MTGSAFAHGRFLIGVDDTDDLESRGTGFHARDLGRRLASAGLAT
                     VEGITRHQLLVDPRIRYTSHNSAACLGVRAAPDSMTALAAACREFLLTASAPGADAGL
                     CIVARGAADAGIQAFGQRAKRAVVERRDAEQLAGGALLEGLTGDGGGVIGALAAVGLR
                     ASGSDGRFLWLPGLREAAERRYRLADLETATGIEAVQTVDGTPIERNEALVDLGSWPR
                     AVLIGGRAVLLVERANHDEPDSWRAAPKELVKRY"
     gene            2191..2688
                     /locus_tag="AC16JC_05755"
     CDS             2191..2688
                     /locus_tag="AC16JC_05755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164236.1"
                     /translation="MLLLLFTGGAAVALHAVTRGHLHLPGHQGFFWMALMGFMRGATG
                     GRWSASIAGVGAGAAAALPVFNFHDPIGVAAYAISGIAFDVLMNLTPEIRYPSLRAGG
                     LAALAFATKPLLQAFATITLGWQTPVARQGLVYPLALHLAFGFAGGLVGTWLWTRVTK
                     SRKIS"
     gene            2685..>3114
                     /locus_tag="AC16JC_05760"
     CDS             2685..>3114
                     /locus_tag="AC16JC_05760"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR5164237.1"
                     /translation="MSRRVALLALAVGAAPATAQQEPGDAYELGPIVVTAPAAGVVST
                     MIVSEQDIARRGARTLDEALALLPGVAIHTGADGVPRIDVRGLRPRQVRVLVNGVPLN
                     STYDGQFDPTMIPTEDIAAITLTTAASSVLYGGGGEVGGVI"
BASE COUNT          449 a         1119 c         1087 g          459 t
ORIGIN      
        1 cctggaactc cgcgtcggcc gcccgcggag tcacgccggg tttcaggcgt gccaccaccc
       61 gcagccagtg ggcgcgccgg aagaagtcct gcgaccggag ctggggatcc cagcgcatgg
      121 gacgccacag gtcgaaggag tcggccgcga aggtgaactc cgcaggtagg acgcccacga
      181 tctcggtgag cgtgccgtcg agtgagacgg tgcggccgac ggcacggtcg gcgcccccga
      241 acaggtcccg ggccagacga tggctgacca cggcgacggg agttcccccg ctccaggtct
      301 cggcgtccgc gaacactcgg ccggcttccg gacgaacctg gaggatcgcg aagtagttgc
      361 cggtcaccga ccgctcgcgc acgcgtcgcg gcgtgtccac gccactcagc gtcgtgcctc
      421 cgccgggggc gtaggccgcc acgtcctcga aggcatgaac ccgctctctc caatcgagat
      481 agttggccgg ggcggcgacc tgccggcgcc accctttctc ggggttgtcc tcggagagca
      541 tgacgaggtg ctcggaatgc gggaacggca gcgggcgcag gatgaccgcg ttgaccgcgg
      601 agaagatggc ggtgttggcc ccgatgccga gcgccagcgt caggatggcg gtgccgctca
      661 gggccggatt gtgccgcagg gtccgcaggg catagcgcgc atcggcgatt gcatcctcca
      721 gccaacgcac gccgcgaccg tcacgatgcg cctccttcac cacctccacc ccgccgaagc
      781 tcacgagcgc cgcccggcgc gccgcctccg gcgcgagtcc caacgcgcgg tgcttctcgg
      841 cttcgagctc cagatgcagg cggatctcct catcgaggtc ccgttccgtc gtgacgcgcc
      901 ggaacagtgc gcgcagccgc agctgccacc cccgcaggat gcccatcagg gagtctccag
      961 gatccagtcg acggccgcca cgtaccggcg ccagctgtcg gtctccgccc cgagcgcgcg
     1021 ccggcccgac gccgtgagcc ggtagtactt ggcgcggcgg ttgtggtcgc tggtgcgcca
     1081 ctcgctgtcg atccagcctc tgagctcgag gcgctgcaac gcaggataga gggagccctg
     1141 gttcacctcg agcacgccgc gcgagatctg ctggatgcgc tggctgatcc cccagccgtg
     1201 catcggctcc agagtgaggg tcttgaggat caggagatcg agggtaccac gcaggacatc
     1261 gcgcgaggga ggagccatcg gtcgcttgtg tcggatttcg acacatagtg cgggctctcc
     1321 tgtcgaaagt caaggggagc gtgcccccga cattgcagcg caagtccctg gctgtagttt
     1381 cccccgggac ctggacggtg acgatgaccg gatcagcctt cgcgcatggc cggttcctga
     1441 tcggcgtgga cgacaccgac gacttggaga gtcgcggcac gggattccac gcacgggatc
     1501 tcggccgtcg gctcgcaagc gccggcctcg ccacggtgga agggatcacc cgccaccagc
     1561 tgctcgtcga cccccgcatc cgatacacct cccacaacag cgccgcctgc ctcggcgtgc
     1621 gcgcggcgcc cgattccatg accgccctcg cggcggcgtg ccgcgagttc ctgctcaccg
     1681 caagcgcccc cggtgcggac gccggactgt gcatcgtcgc ccgcggcgcc gccgacgccg
     1741 ggattcaggc attcggtcag cgcgccaagc gcgccgtggt ggagcgccgc gacgccgagc
     1801 agctcgccgg cggcgcgctg ctcgagggcc tcaccggaga cggcggcggc gtgatcggcg
     1861 cgctcgccgc ggtcggcctg cgcgcgagcg gcagcgacgg acggttcctc tggctcccgg
     1921 gcctgcggga ggcggctgag cgccgctacc gcctcgcgga cctggagacg gcgaccggta
     1981 tcgaagcggt gcagacagtg gacggcacgc cgatcgagcg caacgaggcc ctcgtcgatc
     2041 ttggttcctg gccgcgggcc gtgctgatcg gcggtcgcgc ggtcctcctc gtggaaaggg
     2101 cgaatcatga cgaacccgac tcctggcgag ccgctcccaa ggagctggtc aaacgctact
     2161 gaggccgtcg cgcgccgcga ctgggcgcgc gtgctgctgc tcctcttcac cgggggcgcg
     2221 gcggtcgcgc tgcacgccgt cacccggggc catctccacc tcccgggaca ccagggattc
     2281 ttctggatgg cgctgatggg tttcatgcgt ggcgccacgg gcgggagatg gagcgcctcc
     2341 atcgcgggcg tcggcgccgg cgcagccgcc gccctgcccg tcttcaattt ccatgatccg
     2401 atcggcgtgg cggcgtatgc gatctccggc atcgcgttcg acgtgctcat gaacctcacg
     2461 ccggagatcc gctacccgag tctccgcgcc ggggggctag ccgccctcgc cttcgccacg
     2521 aagccgttgc tccaggcctt cgcgacgatc acactgggat ggcagacccc cgtcgcacgg
     2581 cagggactcg tctacccgct cgcgctgcat ctcgccttcg gtttcgcggg cgggctggtc
     2641 ggcacctggc tctggactcg ggtcacgaag tcccggaaga tctcgtgagc cgccgcgtcg
     2701 cgctgctggc gctggcggtc ggggcagctc ccgcgaccgc ccagcaagag ccgggcgacg
     2761 cctacgagct ggggccgatc gtggtcacgg cgcccgccgc cggcgtcgtc tccacgatga
     2821 tcgtcagcga gcaggacatc gcccggcgcg gagcacgcac gctcgacgaa gcgctggccc
     2881 tgctccccgg tgtggcgatc cacaccggcg ctgacggcgt cccgcgcatc gacgtgcgcg
     2941 gcctgcgccc gcgacaggtg cgtgtgctcg tcaatggcgt tccgctcaac tccacctacg
     3001 acggccagtt cgaccccacg atgatcccga ccgaggacat cgcggcgatc accttgacga
     3061 ccgcagcgag ctcggtgctg tacggcggcg ggggagaggt cggcggcgtg atca
//
LOCUS       DBNGZW010000242         2291 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1522074, whole genome shotgun sequence.
ACCESSION   DBNGZW010000242 DBNGZW010000000
VERSION     DBNGZW010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2291)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2291)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2291
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1522074"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..693)
                     /locus_tag="AC16JC_05765"
     CDS             complement(<1..693)
                     /locus_tag="AC16JC_05765"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0031388 - organic acid phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HIR5164238.1"
                     /translation="MTQTILIAPAGFKGTLRPREVSEALAQGARQALPHAVVLSCPVA
                     DGGDGLLDAVLTPASLREHVRVTGPLGTPVEAELGWIDAETAIFESASACGLALLSPD
                     RYNATESTSRGVGELIVEANDRGAMTVVVGLGGSACVDGGVGAARGLGWTFSDVNGAT
                     LGEGGVALGSLADWEPGWPLKVRVLALADVRAPLVGPQGAAPVFAPQKGASPEAVAQL
                     AAGLDRLAGAMAG"
     gene            complement(1047..>2291)
                     /locus_tag="AC16JC_05770"
     CDS             complement(1047..>2291)
                     /locus_tag="AC16JC_05770"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR5164239.1"
                     /translation="DVGWQPDSATAFALRWTAQTPSDELTALMGMARARSVAEFEAAL
                     VHFRVPEQNVVYADGDGTIAYFLAGLVPVRDSGDGALPAPGWTGAGKWTRYLSAAELP
                     RAVNPPSGFVVTANNRIVGDAFPVPIRADYDLPERAERIWEMVRRDSGATAAAVAREQ
                     VDVVNVFLRRITSIAADALVAACTPPVGGPLLRACGEAGTRGRELAERLRRWDGAMAA
                     DRIEPTLVWSWYRELQALTYDDESPGFRPHAALHRWLLAGSSPWFDDVRTPEHEDLAA
                     LARRAMVGVLSRGDPRPWGEVHTHTEEHPLGEVPVIGRLAGFNVGPLHLGGGNNTVAV
                     CPSSEYQPPFTCTEGPSMRHVVDFGDVDGAGGFILPTGQSGNPRSPHYRDQTGRWLRG
                     ELWIVPVDVGRVRATDTLRLVP"
BASE COUNT          355 a          860 c          768 g          308 t
ORIGIN      
        1 tccggccatc gcccccgcca gccggtccaa tccggccgcg agctgcgcca ccgcctccgg
       61 agaggctcct ttctgagggg caaagacggg ggcggcgccc tgcgggccca cgagcggcgc
      121 gcgaacgtcg gccaatgcca ggacccgcac cttcaagggc catccgggct cccagtctgc
      181 cagcgaaccg agcgcgacgc caccttcccc gagcgtcgcg ccgttcacat cgctgaaggt
      241 ccaccccagc ccacgcgccg ccccgacccc cccatcgacg cacgccgatc cgcccagccc
      301 gaccacgacg gtcatcgccc cccggtcgtt cgcttccacg atcagctcgc ccacgccccg
      361 gctggtggat tccgtggcgt tgtaccggtc gggcgacagt aacgcgaggc cgcacgcgct
      421 ggcactttcg aaaatggcgg tctcggcatc gatccatccc agctccgcct ccaccggtgt
      481 cccgagcggc ccggtcacac ggacgtgctc gcgcaacgag gcgggcgtga gcaccgcgtc
      541 gagcagccca tcgccaccgt ccgcgaccgg gcaggagagg accacggcgt ggggcagcgc
      601 ctgacgggct ccctgcgcca acgcctcgga cacttcgcgg gggcgcagcg tccccttgaa
      661 gcccgcgggc gcgatgagaa tcgtctgggt catggtgggc atcaacggta ggggcgacag
      721 cgtgacggcg ccacacgcgt cgcaacctgc ggcgcggcgc ggaaaacgcg gcactcgcgc
      781 ggtcgtgggc aatttcgcaa ctcattgtta cacaatactt tacggtgaac cggtgagtgg
      841 caccggcctt gcgaagcgtc taggcgaagc cggtgcgttc gcgtcggaca cgggcgcccg
      901 atcgggcgat cggcgagccc ccagggggaa actccggggg gcgttgtttt cttggcgatc
      961 ggctgtcggc tatcggctat cagccgtcaa ctgctgcagc catgatagcc acagcgggat
     1021 agccgatagc tgatagccga tagctttcag ggcaccagcc gcagcgtatc cgtcgcgcgc
     1081 acccgcccca cgtccaccgg cacgatccac aactcgcccc gcagccagcg ccccgtctgg
     1141 tcgcggtaat gcggcgatcg cgggttgccc gattggcccg tggggaggat gaagcccccg
     1201 gcaccgtcca cgtcgccgaa atcgaccacg tgacgcatcg acgggccctc cgtgcaggtg
     1261 aacggtggct ggtactcgga ggacgggcag acggcgacgg tgttgttccc gccgcccaga
     1321 tgcagcggcc ccacattgaa cccggcgagc cgcccgatca ccgggacctc gcccagggga
     1381 tgctcctcgg tgtgggtgtg gacttcgccc cagggccgtg ggtcgcctcg tgacagaacg
     1441 ccgaccattg cgcgtcgcgc gagcgccgcc agatcctcgt gctcgggcgt acgcacgtcg
     1501 tcgaaccagg ggctcgatcc ggccagcagc cagcggtgca gcgccgcgtg gggacggaat
     1561 ccgggcgact cgtcgtcgta ggtgagcgcc tgcaattcgc ggtaccacga ccagaccagc
     1621 gtcggctcga tgcggtcggc cgccatggcg ccgtcccacc ggcgcagccg ctcggcaagc
     1681 tcacgccccc gcgttcccgc ctccccgcac gcccggagca acgggccacc gacgggcggc
     1741 gtgcaggcag ccacgagcgc gtcggccgct atcgacgtga tgcggcgcag aaacacgttg
     1801 accacatcca cctgctcgcg cgccacggcc gcggcggtcg caccactgtc gcgccgtacc
     1861 atctcccaga tccgctcggc acgctccgga aggtcgtagt cggcgcggat cgggaccggg
     1921 aacgcatcgc ccacaatccg gttgttggcg gtcaccacga acccgctcgg cgggttcacg
     1981 gcgcgcggca gctcggccgc cgagaggtag cgcgtccatt tccccgcccc ggtccacccg
     2041 ggcgccggca gggcgccgtc cccggagtcg cggacgggca cgaggccggc gagaaagtag
     2101 gcgatggtcc cgtcgccgtc ggcgtacacc acgttctgct ccggcacgcg gaaatgcacc
     2161 agcgccgcct cgaactcggc gacgctgcgc gcccgcgcca tccccattag ggccgtcagc
     2221 tcatcggacg gggtctgcgc ggtccagcgc agggcgaacg ccgttgcgga gtcgggctgc
     2281 caccccacgt c
//
LOCUS       DBNGZW010000243         2431 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1316491, whole genome shotgun sequence.
ACCESSION   DBNGZW010000243 DBNGZW010000000
VERSION     DBNGZW010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1316491"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            19..1311
                     /locus_tag="AC16JC_05775"
     CDS             19..1311
                     /locus_tag="AC16JC_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase family protein"
                     /protein_id="HIR5164240.1"
                     /translation="MARKKRTSETFIRLKQAFHPESLMMTYGYKPEWSEGALRVPIFQ
                     TSTFVFKTAEEGKAFFEIAYGLRAKTEGEPAGLIYSRINNPDLEILEERLALWDGAES
                     CAVFNTGMAAIATTLMAYVRPGDVVLYSTPLYGGTDHLLKHVLPGFGVTPVPFRASAC
                     AGPDDIAAGLERAQARGRVSLLMVETPANPTNALVDLAACAEFARRQGNGGPRPLVLV
                     DNTFLGPLWQQPLKHGADIVLYSATKYIGGHSDLLAGAALGSHAVLDPVRVLRTFFGT
                     LADPWTGWLLMRSLETLKLRMTSAMKNARYVADFLASHPKVERVYYLGHLQEGDPQYA
                     VYRRQCTAPGAMVSFDVRGGEPEAFRVLNALKLVKLAVSLGGTESLAEHPFSMTHADV
                     SPEAKREAGITERMIRLSVGIEHPDDTIADLEQALAAV"
     gene            1308..1583
                     /locus_tag="AC16JC_05780"
     CDS             1308..1583
                     /locus_tag="AC16JC_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002636169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR5164241.1"
                     /translation="MRDEGRGTRDGWVVYVLRCRDGSLYTGMTNDLDRRLAAHRKGKG
                     GAYTRSRLPVRLVYSERRRSRGAALSREAAIKRLPRSAKLGLLEHGR"
     gene            1580..2263
                     /locus_tag="AC16JC_05785"
     CDS             1580..2263
                     /locus_tag="AC16JC_05785"
                     /inference="COORDINATES: protein motif:HMM:NF014193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial respiratory chain
                     complex IV assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HIR5164242.1"
                     /translation="MRLRQGDLIGAVLALAVVALFVRLGLWQIARLRERQAQNAIIAA
                     RRELPPVELPMHHFQADALRDRRAHARGVYDYAREHVWGGRTFEGAPGVALLTPLRLS
                     DGSAVFVDRGFATSPDAAHVDQARYREGDTVEVSGLILPAPRDRRDVNPDVLRDSLPY
                     ALLPAVVQVDDSAAPHPPGLHRWPPPTLGSGSHLSYAIVWFSLALISLVGTGQLLWMR
                     AREGLPPLA"
BASE COUNT          357 a          821 c          829 g          424 t
ORIGIN      
        1 gcccgctagc ttcacgccat ggcacggaaa aagcgcacgt cggagacgtt catccgactg
       61 aagcaggcgt tccatcccga aagcctgatg atgacctacg gctacaagcc ggagtggtcg
      121 gagggcgcgc tcagggtccc gatattccag acctccacgt tcgtcttcaa gaccgccgaa
      181 gaaggcaagg ccttcttcga gatcgcctac ggcctccggg cgaagaccga gggcgagcca
      241 gcgggattga tctacagccg catcaacaac cccgacctgg agattctgga ggaacgcctg
      301 gcgctgtggg acggcgcgga gtcgtgcgcc gtgttcaata cggggatggc ggcgatcgcc
      361 accacgttga tggcctacgt gcgccccggc gatgtcgtgc tgtacagcac gccgctctac
      421 ggcggcaccg atcacctgtt gaagcacgtg ctgccgggat tcggcgtgac gcctgtgccg
      481 tttcgcgcct ccgcctgcgc cgggccggac gatatcgccg ccggcctcga gcgcgcccag
      541 gcgcgggggc gggtatcgct cctgatggtc gagacgcccg cgaatcccac caatgcgctc
      601 gtcgatctcg ccgcctgcgc ggagttcgcc cggcgccagg gaaacggcgg accgcggccg
      661 ctggtcctgg tggacaacac gttcctcggc cccctctggc agcagccgtt gaagcacggt
      721 gccgacatcg tgctctactc cgccaccaag tacatcgggg gccacagcga tctcctggcg
      781 ggcgccgccc tgggcagcca tgcggtgctc gatccggtgc gggtgctgcg caccttcttc
      841 gggaccctgg ccgacccatg gaccgggtgg ctgctgatgc gcagtctcga gaccctcaag
      901 ctgcggatga cgtcggcgat gaagaacgcg cgctacgtgg cggactttct cgcgtcgcac
      961 cccaaggtcg agcgggtcta ctacctcggt cacctgcagg agggcgatcc gcagtacgcg
     1021 gtctatcgcc ggcagtgcac ggcgcccggg gccatggtgt cgttcgacgt gcgcggcggc
     1081 gaacccgagg cattccgcgt cctcaacgcg ctgaaactgg tcaagctcgc ggtgagcctg
     1141 ggtgggaccg agtcgctggc cgagcacccg ttttcgatga cgcacgcgga cgtctcgccc
     1201 gaggcaaagc gcgaggcggg gatcaccgag cgcatgattc gcctgtcggt gggcatcgag
     1261 catccggacg atacgatcgc ggacctggag caggcactgg ccgctgtatg agggacgagg
     1321 gacgcgggac gagggacggg tgggtcgtct acgtgctgcg ctgccgggat ggatcgctct
     1381 acaccgggat gaccaacgat ctcgatcgcc gtctggccgc tcaccggaag gggaagggcg
     1441 gggcctacac gcgctcccgc ctcccggtgc gcctcgtcta ctcggagcgg cgccgtagcc
     1501 gcggtgcagc cctgtcgcgc gaggcggcga tcaaacggtt gccgcgctct gccaagctgg
     1561 ggctgctgga gcacgggcgg tgagactgcg gcagggcgac ctgatcggcg cggtcctcgc
     1621 cctcgccgtg gtcgcgctgt tcgtgcgact tggcttgtgg cagatcgcgc gattgcgtga
     1681 gcggcaggcg cagaatgcga tcatcgcggc gcgccgcgaa ctgccgccgg tcgagcttcc
     1741 tatgcaccac ttccaggcag atgcgttgcg cgaccgtcgc gcgcacgcga ggggggtcta
     1801 cgactacgcc cgcgagcacg tgtggggcgg tcggaccttc gagggtgcac cgggcgtcgc
     1861 actgctcacg ccgctgcggc tctctgatgg ctccgcggtc ttcgtcgatc gcggctttgc
     1921 gacctcgccc gacgcggcgc atgtagacca ggcacgttat cgcgagggcg acaccgtcga
     1981 ggtgtcggga ctgatcctgc cggcgccgcg cgatcgccgc gatgtgaatc ccgacgtgct
     2041 gcgggattcg ctgccgtacg ccctactgcc ggccgtggtg caggtggacg attccgctgc
     2101 tccgcatcct ccgggactgc accgctggcc tcccccgaca ctcggcagcg gatcccattt
     2161 gtcttacgcc atcgtgtggt tcagcctcgc tttgatctcc ttggtgggaa caggacagtt
     2221 gctgtggatg agggcgcgcg agggcctgcc accgctcgct tgacgtcgat tcttcgggcc
     2281 tccatcctta cgtcgctcgt ttcacacgcc gtcgttccca catcccggag ccagtccatg
     2341 cgccgagtgc tgaccgcgac ggttctcgcc gctgtccttc ctgctgttcg tcctcacgtt
     2401 tcactgccgg cgtcgcctcc ttcggcctgg a
//
LOCUS       DBNGZW010000244         2531 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_294367, whole genome shotgun sequence.
ACCESSION   DBNGZW010000244 DBNGZW010000000
VERSION     DBNGZW010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_294367"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..573)
                     /locus_tag="AC16JC_05790"
     CDS             complement(<1..573)
                     /locus_tag="AC16JC_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164243.1"
                     /translation="MILANVRARLRGSDCRLVLVALGRREPGARARAEHRLATEGPDG
                     LLDEPGLLEALLALRSLAVPSAALFAYVTVRHALLAADLDDRPLADYLAALLLEFGRG
                     DRASRPEPADDQTYHYLTEIVNDLAQDQGSSERRFLLQAHLGNYALWLAGLFPDFIAV
                     RRARRGGPDLPYYDALGRRGFGAAAEHSLAK"
     gene            572..2530
                     /locus_tag="AC16JC_05795"
     CDS             572..2530
                     /locus_tag="AC16JC_05795"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR5164244.1"
                     /translation="MHQASSTSTPPTPPSQADLLARLTALSRELAGAFRPATVIERLA
                     AAITELLRPDRLTIVAQDVDSNRMQIAYHHGGDRPHIEDPLLQLPLRRGPMVFTRHVL
                     EEAARHGVTVTQDAAACMGAPIQAVGRSIGAVTVGSRRAGAFGPLELALLTALVAQAA
                     IALENARLLQLLSSGKRQWELTVDAINQAICIVDEQGVVRRANRVFADLVHLPVTALP
                     GRPWLSLVPPAWGSVVSRALVEPGAHEIRTPERVYTVSAIRMEGADDEAAVLSFEDHT
                     EKRRLQDQLIQSEKMSAIGQLIAGIAHDLNNPLASVVGFADFLAESGDVPPALAEPLR
                     VIRQEAERAATIVKNLLSFARRQEGERLPQSLQPLLESVLALLKNQMMALKVETTLTI
                     EPGIPDIEMNPNQVTQVFVNLLNNAAQAIASTGKAGHITVTAKRWLDGVAVSVADDGP
                     GIPEELMPRVFEPFFTTKTEGEGTGLGLSICQGIVKEHGGRITLESSPGAGATFTVEL
                     LGGSRPPRPSQPVPAVTAGGALRVLVVDDEPHILHYMRATLESWGHTVEVASDGTEAV
                     ERALVTPFDVIICDLRMPRLGGREMYQNLERAHPHIARKVIFATGDTVRGDTLQFLET
                     LGRPFLHKPFTLAELRTVLGSVAAEAQQ"
BASE COUNT          400 a          891 c          890 g          350 t
ORIGIN      
        1 cttcgccagc gagtgctccg ccgcggcgcc gaacccccgg cgaccgaggg cgtcgtagta
       61 cggcaggtcc ggtccaccgc ggcgcgcgcg ccgcaccgcg atgaagtcgg gaaacagccc
      121 cgccagccac agggcgtagt tgcccagatg tgcctgcagc aggaagcgcc gctcggaaga
      181 gccctgatcc tgggccagat cgttcactat ttcggtcaga tagtggtagg tttggtcatc
      241 ggccggctcg ggccgggagg cacggtcccc ccgaccgaat tcgagcagca acgcggccag
      301 atagtcggcc aggggacggt cgtccagatc ggcggccagc agcgcgtggc gcaccgtcac
      361 gtaggcgaac aacgccgccg atgggacggc gagcgagcgc aacgccagca gggcctcgag
      421 cagccccggc tcgtccagca ggccatcggg gccttccgtg gcgagacggt gctccgcgcg
      481 ggcccgtgcc cccggttccc ggcgccccag ggcgaccaga accagtcggc aatccgaccc
      541 gcgcaggcgg gctcgaacgt tcgcgaggat catgcaccag gcctcatcga cttctacacc
      601 tccgacgcca ccgtctcagg ccgatctgct cgcccggctc accgcgctgt cgcgcgagct
      661 ggccggcgcc ttccggccgg ccacggtgat cgagcgactg gccgcggcga tcaccgagct
      721 gctcaggccg gaccgcctca cgatcgtcgc ccaggacgtg gactccaata gaatgcagat
      781 cgcgtaccac cacggtggtg accggccgca catcgaggac cccctgctgc agctgccgct
      841 gcggcgcggc ccgatggtgt tcacccgtca cgtgctggag gaggccgcgc gacacggcgt
      901 gacggtgacg caggatgccg cggcctgcat gggcgcgccg atccaggcgg tcggccgcag
      961 catcggcgcc gtgacggtcg gctcccggcg ggccggggcg ttcggacccc tggagctcgc
     1021 gctgctgacg gccctggtgg cgcaggcggc catcgcgctg gaaaacgccc gtctgctcca
     1081 gttgctctcc tcgggcaaac ggcagtggga gctgacggtc gacgccatca accaggcgat
     1141 ctgcatcgtg gacgagcagg gggtcgtgcg tcgcgccaac cgcgtgttcg ccgatctggt
     1201 gcacctcccg gtgacggcgc tgcccggccg cccctggctg agcctcgtcc cgccggcatg
     1261 gggttcggtg gtctcgcgcg cgctggtcga gcccggggcg cacgagatcc gcaccccgga
     1321 gcgggtgtac acggtgagcg cgatccggat ggagggcgcc gacgacgagg cggcggtgtt
     1381 gtcgttcgag gaccataccg agaagcggcg gctccaggat cagttgatac agtctgagaa
     1441 gatgtcggcg atcggtcaac tgatcgccgg tatcgcccat gacctgaaca accccctcgc
     1501 ctcggtggtg gggttcgccg acttcctggc cgagtcgggg gacgtgccgc cggcgctggc
     1561 cgagccattg cgcgtgatcc gccaggaggc ggagcgcgcc gcgacgatcg tgaagaacct
     1621 gctgtcgttc gcgcgccgcc aggaagggga acggctgccg cagtccctgc agccgctgct
     1681 cgagtcggtg ctggccctcc tgaagaacca gatgatggcg ctcaaggtcg agacgacgct
     1741 cacgatcgag ccgggcattc ccgacatcga gatgaacccg aatcaggtca cgcaggtgtt
     1801 cgtgaatctg ctgaacaatg ccgcgcaggc catcgcgagc acgggcaagg ccggccacat
     1861 cacggtgacg gccaaacgct ggctcgacgg cgtcgcggtc agcgtcgccg acgacggacc
     1921 cggcatcccg gaggagctga tgccgcgggt gttcgagcct ttcttcacca ccaagaccga
     1981 gggcgaaggg acgggactgg gcctctcgat ctgccagggc atcgtgaagg agcacggcgg
     2041 acggatcacg ctcgaatcga gtcccggcgc cggggcgacg ttcaccgtcg agctgctggg
     2101 cgggagccgg ccgccgcgcc cttcccaacc cgttcccgcg gtcaccgccg gcggcgccct
     2161 ccgcgtgctg gtggtggacg acgagccgca catcctgcac tacatgcgcg cgacgctcga
     2221 gagctggggc cacacggtcg aggtcgcgag cgacggaacg gaggccgtgg agcgggcgct
     2281 ggtgacgccg ttcgacgtca tcatctgcga cctgcgcatg ccgcgcctcg gcggacgcga
     2341 gatgtaccag aacctggagc gcgcccaccc gcacatcgcc cggaaggtca tcttcgccac
     2401 cggcgacacg gtccgcggcg acacgctgca gttcctcgag accctgggcc gccccttcct
     2461 ccacaagccg ttcaccctgg ccgagctccg cacggtcctg ggcagcgtcg cggccgaggc
     2521 ccagcagtaa t
//
LOCUS       DBNGZW010000245         3492 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_908080, whole genome shotgun sequence.
ACCESSION   DBNGZW010000245 DBNGZW010000000
VERSION     DBNGZW010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3492)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3492)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3492
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_908080"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2100)
                     /locus_tag="AC16JC_05800"
     CDS             complement(<1..2100)
                     /locus_tag="AC16JC_05800"
                     /inference="COORDINATES: protein motif:HMM:NF017930.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HIR5164245.1"
                     /translation="MTRAYLLSNGRYRVILTDAGGGYSAWQNVALTRWAADRSRDVGG
                     VRFYLRDLEREYCWSAGRNPIAGKPDQYQTARDGGSVAIVREECEIVSRLDVVVDPES
                     DCEYRILALTNRSRTERRLDVTSYLEVTLNDSAADAAHPAFSKLFVETEFSADAKALL
                     ARRRPRSPDDLPLWMGHGFWLEGGAPGDVEVETDRARFIGRGRSLADPVALDKGTRLS
                     GSVGPVLDPVFSLRRAVRLAPGGTARLVTLVAAGERRDDLARRLTAPPPRRIDAVLEA
                     ARRLDLPADPTRLATLTGALLFGAAPVVTPAELPKASPLTPGDLAALGVPANVPLVLA
                     AVRGDGDRAGLQSLSQVVRWWRQNGLAVTLLVLEDGASAGGAVDRDPANVVRSASEVA
                     PELLILAQRCARLSLDRLRDPAPTLGIVSNAEPPILDPHCAAILEATEELRAFNGFGG
                     FTADGSEYVIRLDRVDGRLRLPPRPWTNVVANETAGFIASETGAGYTWTVNSRENRLT
                     PWSNDPVLDPPGEALYLRDEDRDVCWSPTPGPLPDGGAYECRHGFGYTVWRHTSLDLE
                     QETTAFVPRHDAVKLVRVRVANRGSAARRLSLTYHARWVLGTTPEQDGRFVVTQHDAD
                     TGAILAVHPHRGPLAGRVAFACVAGPQGRGDTASHTADRAALLAAERSGAPLDGRTGA
                     GLDPCAAFRVPFTLAGGA"
     gene            complement(2108..>3492)
                     /locus_tag="AC16JC_05805"
     CDS             complement(2108..>3492)
                     /locus_tag="AC16JC_05805"
                     /inference="COORDINATES: protein motif:HMM:NF036578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-rhamnosidase-related protein"
                     /protein_id="HIR5164246.1"
                     /translation="DVTIDVPADTQLTDAIRASAGWILATRKGAALEPGTRAYDRSWI
                     RDGALMAAALLRFGYPDVVRDYIAWYAPHQYANGKVPCCVDARGADPVPEHDSNGEFI
                     YLVMEYWRHTGDRSLLERMWPHVVRAVAYLDSLRQTRRTPQYRTGDAQVFFGLLPPSI
                     SHEGYSAKPMHSYWDDFFALRGFKDAATMAAVLGHTAEATRFAAFRDEFETDLLRSLE
                     VAMTQHTIDYLPGAADLGDFDATSTTIALSPVGEENRLPQAALERTFERYWTNLQGRL
                     EDSAWDGYAGYEIRAAGAMLRLGWKARSLALLRAFLNDREPPAWNQWPEVVYRDRHAP
                     KFVGDVPHAWVAADFLRSASDLFAYERESDSALVLGAGLDSARLGGSGIRVAVHTWWG
                     PLRYSVRREGNTVRFRIEVGVYLPPGGLVVRAPLPGRRAVFVDAAEAVSDSAGGVVVR
                     RVPLDIVFQY"
BASE COUNT          529 a         1215 c         1263 g          485 t
ORIGIN      
        1 ggcgccgccg gcgagcgtga acgggacgcg aaacgcggcg caggggtcga gcccggcgcc
       61 ggtccggccg tcgagcggag cgccgctgcg ctctgccgcc aggagcgcgg cacgatccgc
      121 cgtgtgactt gcggtatcgc cgcggccctg gggacccgcg acgcacgcga acgcaacgcg
      181 tcccgccagc ggaccgcggt gggggtgcac ggcgagaatg gcgccggtgt cggcatcgtg
      241 ctgcgtgacg acgaagcgtc cgtcctgctc gggcgtcgtc cccagcaccc agcgcgcgtg
      301 ataggtgagg gagagtcggc gcgcggcaga gccacgattg gctacgcgca cccgcaccag
      361 tttcaccgcg tcgtgccgcg gcacgaacgc cgtggtctcc tgttcgaggt cgagactcgt
      421 gtgccgccag accgtgtagc cgaacccatg acggcactcg tacgcgccgc cgtcggggag
      481 cggcccgggc gtgggcgacc agcagacgtc gcggtcctcg tcgcggagat acagcgcctc
      541 gccaggggga tcgagcaccg ggtcgttgct ccacggcgtc agccggttct cgcggctgtt
      601 cacggtccag gtgtagccgg cgccggtctc actcgcgatg aaccccgccg tctcgttggc
      661 gacgacgttg gtccagggac gcggtgggag ccgcagccgg ccgtcgacgc gatcgagccg
      721 gatgacgtac tccgagccgt cggcggtgaa ccctccgaat ccgttgaacg cccgcagctc
      781 ctcggtcgcc tcgagtatgg cggcacagtg gggatcgaga atcggcggct ccgcattcga
      841 aacgatgccg agggtgggtg cgggatcgcg cagccggtcc agcgacaacc gagcgcaacg
      901 ttgcgccagg atcaacagct cgggcgcgac ctcggaggcg gagcggacga cgttcgcggg
      961 atcccgatcg accgcccccc ctgcactggc gccatcctcc aggacgagca gcgtcacggc
     1021 cagtccgttc tgccgccacc atcgcacgac ctggctgaga gactggagcc cggcccgatc
     1081 tccgtcaccc cgcacggctg ccagcacgag gggcacgttg gccggcacac cgagcgcggc
     1141 aaggtcgccc ggggtgagtg ggctggcctt cggcagttcc gccggggtca cgactggtgc
     1201 ggcgccgaac agcagcgcgc cggtgagagt ggccagccgg gtcgggtcgg cggggagatc
     1261 gagtcgtctg gcggcctcca gcaccgcgtc gattcggcgc ggaggtggag cggtcagtcg
     1321 gcgcgccagg tcgtcgcgcc gctcgcctgc ggccacgagc gtcacgagcc gggcggtgcc
     1381 tcccggagcg agccgcacgg cgcgccgcag ggagaacacg ggatcgagga ccgggccgac
     1441 actccccgac agtcgggttc ctttgtcgag cgccacgggg tcggccagag agcgtccccg
     1501 gccgatgaac cgcgcgcggt ctgtttcgac ctcgacatcg cccggcgcgc cgccctcgag
     1561 ccagaatcca tggcccatcc acaggggaag gtcgtcgggg ctgcgcggcc gccgtcgcgc
     1621 caggagcgcc ttggcatccg cggagaactc ggtttcgacg aacagcttgg agaaggcggg
     1681 atgcgcggcg tcggcggccg aatcgttgag cgtcacctcg agatagctcg tgacgtcgag
     1741 ccggcgttcg gtccgactgc ggttggtgag cgcgaggatg cggtattcgc agtccgactc
     1801 cggatcgacg accacgtcca gccgggatac gatctcacac tcctcccgta cgatggcgac
     1861 ggatcccccg tccctcgctg tttggtattg gtccggtttc cctgcgatcg ggttacgccc
     1921 cgccgaccaa cagtattcgc gctccaggtc gcgcaggtag aaccgcacgc cgcccacgtc
     1981 tcgcgaacga tccgccgccc atctcgtgag cgccacattc tgccaggccg aatagccgcc
     2041 gcccgcatcg gtcagaatca cacgatagcg gccgttcgag agcaggtacg cgcgcgtcac
     2101 gtgccggtca gtactggaag acgatgtcga gcgggacccg gcgcacgacg actccgcccg
     2161 ccgagtcgga caccgcttcg gcggcgtcga cgaagacggc tcggcgtccg ggcagcggcg
     2221 cgcgcacgac caacccgccg ggtggcaagt acacgcccac ctcgatacgg aaccggaccg
     2281 tattgccttc gcgccgcacc gagtagcgca gcggacccca ccaggtgtgc acggctacgc
     2341 ggatgccgct gccgcccagc cgggccgaat cgaggccggc gccgaggacg agcgcgctgt
     2401 ccgattcgcg ctcgtaggcg aacaggtcgc tggccgaccg caggaagtct gccgcgaccc
     2461 aggcgtgcgg cacgtcgccg acgaactttg gggcgtggcg gtcgcggtag accacctcgg
     2521 gccactggtt ccacgcggga ggctcgcggt cgttcaggaa cgcccgcagc agcgcgagcg
     2581 accgcgcctt ccagccgagg cgcagcatcg cgccggcggc gcggatctcg tagcccgcgt
     2641 agccgtccca ggcactgtcc tcgagcctgc cctgcaggtt ggtccagtag cgctcgaacg
     2701 tgcgctcgag cgcggcctgc gggagccggt tctcctcccc cacgggggaa agcgcgatgg
     2761 tggtggacgt ggcgtcgaag tcacccaggt cggcggcgcc gggcaggtag tcgatcgtgt
     2821 gctgcgtcat cgcgacctcc agcgagcgca gcaggtcggt ctcgaactcg tcccggaacg
     2881 ccgcgaatcg cgtcgcctcg gcggtgtgac cgagcaccgc ggccatggtc gcggcgtcct
     2941 tgaacccgcg cagcgcgaag aagtcgtccc agtaggagtg catcggcttc gcggaatagc
     3001 cttcgtggct gatcgacggc ggcaacaggc cgaagaacac ctgcgcatcg ccggtgcgat
     3061 actgcggcgt gcggcgcgtc tggcgcagcg aatcgaggta ggcgaccgcg cgcaccacat
     3121 ggggccacat gcgctcgagc agcgaccggt cgccggtgtg gcgccagtat tccatcacga
     3181 ggtagatgaa ttcgccgttc gagtcgtgct ccggcaccgg atcggcgccg cgcgcatcca
     3241 cgcagcaggg caccttgcca ttggcgtact ggtgcggggc gtaccaggcg atgtagtcgc
     3301 ggaccacgtc cgggtagccg aatcgcagca gtgccgccgc catcagggcc ccgtcgcgga
     3361 tccaggagcg gtcgtaggcg cgcgtgccgg gctcgagcgc cgcgcccttc cgggtcgcga
     3421 gaatccagcc ggcgctcgcc cggatggcgt cggtgagctg cgtgtcggcg ggcacgtcga
     3481 tcgtgacgtc gc
//
LOCUS       DBNGZW010000246        17165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1182686, whole genome shotgun sequence.
ACCESSION   DBNGZW010000246 DBNGZW010000000
VERSION     DBNGZW010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 17165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17165
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1182686"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(179..2056)
                     /locus_tag="AC16JC_05810"
     CDS             complement(179..2056)
                     /locus_tag="AC16JC_05810"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5164247.1"
                     /translation="MTQGLSSPEFVALEAALRGRYVLERKLGRGGMGVVFLARDVALD
                     RPVAIKLLPPALATPQQRERFLQEARTAARLAHPHIVPIHAVEQHDDLVFFVMGYIAG
                     MTLAERVRTQGPLDPAAATRMVQEVAWALAHAHQHGVVHRDIKPENILLERGTGRALV
                     TDFGIARLGAETPPTGSGRVLGTARFMSPEQAAGEVVDGRSDLYSLGVTAFYALTGRL
                     PFDAADAHGLMVQRETLPAPPVTSVRAGLPPALAAAIDRCLAKTPADRFTTAGDLADA
                     VAAVREGTAVPATLQRITREISSFAVDLAGYGTLAAVAIVSKFLFSNEFDFFGVRTVY
                     TIAIALVLVSLTALRGMSMFRLIREAVAEGWGERDLAAAIEREARAAGSTVRQPRPGR
                     LGATAIFLVGFAALVAYWLGPKEAGLSSLEGPLAWLVELVGLIAPVAWGRWYGSRLEA
                     PRDGKPGLFSRLVMRVKMKGFFVLARLGRRLRTPAVVAPADQHTEVLIADAARDILHA
                     LPDPDRRLVGVEQLLTRLEADAAVLRRRMGELDQAAAQVGAAASSRRDALAHDLEAAR
                     RTAAERLGTTVSAMESLRLDLLRLRAGIGSTDGLTEDLEAVRELAARVDAEAELEADT
                     K"
     gene            2161..4446
                     /locus_tag="AC16JC_05815"
     CDS             2161..4446
                     /locus_tag="AC16JC_05815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR5164248.1"
                     /translation="MDRLEGLAKLTGRERYVDDLPLDDFLWGATVRSPAPRGRITAVR
                     FGPGVDWSQFTLVDHRDLPGPNVIALIENDQPVLAAGSVRHVHEPVLLLAHPDRAALR
                     RAVAAVTIQVAPEPAALDFRLPPAPETIQYGSDNILKHLSISKGDVERALAAAPIVVE
                     GTYETGAQEHVYLEPQGMIAQVDGDVLVLRGSMQCPYYVLKALRTALARDDRHLRVVQ
                     TPTGGGFGGKEEYPSTIALHAALLALKSGRPVKMIYDRTEDMAATTKRHPALVRHRTG
                     VATDGRLLAQDIDVIMDGGAYVTLSPVVLSRGIIHAAGPYACDHVRIDGRAIFTNAVP
                     FGAFRGFGAPQTHFANERHMDRIAARLGLDPAALRRRNLLRDGQSTATGQVIQDGTDR
                     VALLDRALSLADYDAKRTAHAAHNAASATTRRGIGIATFHHGAGFTGSGEVALASRLH
                     VAGLPDGRVEVRSANIEMGQGTLTVFTALAAERLGLELSEVVIAEADTARVPNSGPTV
                     ASRTAMVVGHLVEQACDALRARVGLDAGATGQTVRDALRRWHERHPGEDLVGEAVYQR
                     PPGISWDETTYRGDAYATFGWGAYVAEVEVDLRTWQTRVIGFVALQEVGHVLNDCLAR
                     GQIQGGVAQAIGWALFEECRWHDGAMANAQLTNYLIPTSDDLPPIRVAFVENPYPLGA
                     QGAKGLGELPMDGPAAAILNAVAAATGADPCAIPLTPERLMKIADWGLRIADSKGEGT
                     VPGAVNPQAAIRNPQSRSPSL"
     gene            4458..4520
                     /locus_tag="AC16JC_05820"
                     /pseudo
     CDS             4458..4520
                     /locus_tag="AC16JC_05820"
                     /inference="COORDINATES: protein motif:HMM:NF012339.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            4590..4871
                     /locus_tag="AC16JC_05825"
     CDS             4590..4871
                     /locus_tag="AC16JC_05825"
                     /inference="COORDINATES: protein motif:HMM:NF013923.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR5164249.1"
                     /translation="MNSCLVPIYQVRGCRVETVESLDPAVLRPLLASGATQCGACTPG
                     VAMTACWIREHRELLASHSVRELLAGNLCRCTGYDGIVDGIRASLEARA"
     gene            4868..5683
                     /locus_tag="AC16JC_05830"
     CDS             4868..5683
                     /locus_tag="AC16JC_05830"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR5164250.1"
                     /translation="MKLLLPESRDQALALLTATPAAVPMAGGTDLLVHWPLRTAEHDR
                     TYLDLSPLRDLKGMRWEDGALVLGGLATYWDVIRDPRTAQELPLLVEAARQVGAVQIQ
                     ARGTWAGNIVNASPAADGVPVLMAYDAIVVLESVRGRAEVPLAELYTGYKQLRRAPDE
                     LVVAIRIPRRVYALQRFFKVGARRAQAIAKVGLAVTRSSAGWRVVAASMAPTIRRCPS
                     LERLLEEGADIRSPDDLLPVLAQDVSPIDDIRSSATYRRQVMARVLYDAVRGS"
     gene            5680..6627
                     /locus_tag="AC16JC_05835"
     CDS             5680..6627
                     /locus_tag="AC16JC_05835"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015206935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR/zinc-dependent alcohol dehydrogenase-like
                     family protein"
                     /protein_id="HIR5164251.1"
                     /translation="MKGVWLEDRRLRLRDDLPVPEPPAGEALVRVLAAGICNTDLELV
                     RGYYPYTGVPGHEFAGEVMSDGALAGKRVVGEINAVCGDCRACRAGRRSHCERRTVLG
                     IVNRHGAFAEYLTLPVENLHVIPDGVTTEVAVFTEPLAAALEIQEQVAIAPTMRVLVV
                     GDGKLGQLVAQTLALTGCDLVVSGRHEAKLDLLRRRGIATVTPGGIAAGAFDVAVECT
                     GNPEGFAVAQRALRPRGTLVMKSTYADKLTLDASAVVVHELTLIGSRCGPFPKALALL
                     AAGQVDVLPLVSARYPLDRALDAFEHAQRPGTLKVLLGR"
     gene            complement(6631..7824)
                     /locus_tag="AC16JC_05840"
     CDS             complement(6631..7824)
                     /locus_tag="AC16JC_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012700713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HIR5164252.1"
                     /translation="MSAHLVQWLSLVLRWAHVITGVSWIGTSFYFNWLNWRIAPLETP
                     EPGVGGELWSVHGGGFYRVVKYSVAPARLPRTLHWFKWEAYATWWSGLSLLVLLYYAG
                     AREYLLDPAVSSIGPMAAVMLGVGTLVVCWLVYDLLCRSPIAGRPAALAVLGLVLVTA
                     LAWGLGKVFSARAAYLHVGAALGTIMAANVFRVIIPAQRDMVAALSAGTAPDAARGRQ
                     GALRSLHNNYLTLPVLFVMVSNHYPATYGSGWGWAILGVLFVIGVLVRHWFNLRNAGR
                     RNAWLLPAAVAGLMILAIVTAPRPGGGTPAGSLGSVSFAEVRVVIARRCAPCHSTAPT
                     YPGITAAPAGVILDTPEQIRARAARIVERAVLQQTMPLGNVTGITAEERALLGRWVRA
                     GASLR"
     gene            complement(7828..8166)
                     /gene="uraH"
                     /locus_tag="AC16JC_05845"
     CDS             complement(7828..8166)
                     /gene="uraH"
                     /locus_tag="AC16JC_05845"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012794337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033971 - hydroxyisourate hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006144 - purine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HIR5164253.1"
                     /translation="MRLSTHVLDTARGRPAPGVAVTLSAQGPRGWAELARGTTDADGR
                     IPTLLPATAPAAAGTTFRLRFEVGEYFATRGEAAFYPYVEVVFTPHDDGHYHVPLLVS
                     PFGYSTYRGS"
     gene            complement(8171..8650)
                     /gene="uraD"
                     /locus_tag="AC16JC_05850"
     CDS             complement(8171..8650)
                     /gene="uraD"
                     /locus_tag="AC16JC_05850"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006972807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HIR5164254.1"
                     /translation="MAAYLNALPRSEAETELRRCCGSRRWVERMLAGLPYASDAALLE
                     AAEREWWTLARADWLEAFAAHPRIGERSGDAWSRGEQSSVDQAASDVRRALEQGNRAY
                     EARFGHVYLVCATGRGAADLLADLRRRLVADPEQELRTAAGEQAKITALRLEKLGGE"
     gene            complement(8686..9681)
                     /gene="alc"
                     /locus_tag="AC16JC_05855"
     CDS             complement(8686..9681)
                     /gene="alc"
                     /locus_tag="AC16JC_05855"
                     /EC_number="3.5.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004037 - allantoicase activity [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoicase"
                     /protein_id="HIR5164255.1"
                     /translation="MNGGLIDLAAARVGGRAVLANDEFFAPKSNLLNPGPPVFAAGKY
                     TSRGKWMDGWETRRRRTPGSDWCIVRLGVPGVVRAVTVDTRHFRGNHPEACSLDAATL
                     KGKDDATFKRRGGGAVAWATLLPRAPLAGDALNDFTVTGESRITHVRLTIYPDGGVAR
                     LRLWGEARPDWAALEKKTRPLDLAALRNGGAPLAASDRFFSDPLNLLMPGGPEDMSDG
                     WETRRRRGPGHDWVILRLGARGTIDAVRVDTTHFKGNFPESCRLEGCDLPGLEANAVP
                     DEGAPWRELVPRTQLKADAQHRFRAVGRSGPVTHVRFSIFPDGGVARLRLYGRRV"
     gene            9753..9977
                     /locus_tag="AC16JC_05860"
     CDS             9753..9977
                     /locus_tag="AC16JC_05860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164256.1"
                     /translation="MAEKSYSGPERRKAKRRKRADRRNVFDRRRWKERRRTWGRTSTE
                     RRVDKDRRSLVPRRGGDERRTGRDRRGSDE"
     gene            complement(9978..11348)
                     /gene="allB"
                     /locus_tag="AC16JC_05865"
     CDS             complement(9978..11348)
                     /gene="allB"
                     /locus_tag="AC16JC_05865"
                     /EC_number="3.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002632115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004038 - allantoinase activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0050897 - cobalt ion binding [Evidence
                     IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HIR5164257.1"
                     /translation="MKPDLVISSGRVVTPEGERPGAVVVAQGRIIAVVPPGEAPAAAE
                     THDVGAAALLPGLVDSHVHVNEPGRTEWEGFASATRAAAAGGVTTIVDMPLNSVPPTT
                     TVAALETKARAAAGQAMVDYAFWGGAVPENLDDLEPLSRAGVPGFKCFLVPSGVEEFS
                     HLTESDLVATMHRLAELDQVLLTHAELPGPISEAEASLTGADPRRYATWLAARPRGAE
                     LEAIDLLLLLSLETGCRVHIVHLACAAALDRIANARRAGARVTVETCPHYLSFAAEDI
                     PDGQTAFKCAPPVRERENRERLWHGLAHGAIDLVASDHSPAPPALKHLETGDFLAAWG
                     GIASLELSLAAIWTGARARGHGLPALARWLSQGPAHLAGLTRKGRIAPGYDADLVVFD
                     PGHGWTVDAERLHQRHPLTPYAGLALRGKVLRTFLRGICVYEEGRFPAPPGGTWLRRE
                     DRSGRA"
     gene            complement(11345..11857)
                     /locus_tag="AC16JC_05870"
     CDS             complement(11345..11857)
                     /locus_tag="AC16JC_05870"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5164258.1"
                     /translation="MPPYNTMLEETLEAWQGVREGLIAEVRNIPASRFDFRPTPQSRS
                     VAELVQHILEVAMMMTGELTRPDTNFHRAPWPKLLGLYARAARRARTKPMLLRLLTSQ
                     LKDGERRFRRAGELSLLQLITRFDGLPGTRLAWWNHGIAHEEYHRGQVALYARLMGIE
                     PALTRLIRGG"
     gene            complement(11927..13930)
                     /locus_tag="AC16JC_05875"
     CDS             complement(11927..13930)
                     /locus_tag="AC16JC_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5164259.1"
                     /translation="MMVEANGVLSPGEHAVLDAVCETLLPSISRADDPAGFFAAGARA
                     LGTADRAVRLIGSIPDPGDRDRLRLLLRTLDNPFVNLLFTGRFARFTAMQPPAREAVL
                     RGFAHSHIALRRAGFQALKRLVHVAHYCWPPSAGTRHPAWDANGYPGPLPQPAKCLEP
                     LPTLSVDRDTVLECDAVVVGSGAGGGVMAGLLAEAGMGVVVLEKGGNPGSRDFTQVEG
                     DMLSRYYLDGGLIMTQSGSLPILAGSCLGGGTAINYTTSFPLPPTTRAEWDRRSGLTL
                     FTSPRFQRSFDRVAIQVDVGTRYTTPGVRDQLLERGCKMLGWHVDLIPRNVTDCREGV
                     ECGYCGYGCRHGAKNSTARTYLAMAARHGAHLVPHCDVDRVLVATGRAGGVEGTVAGP
                     DEARHRLTVRAPIVVAACGSINTPALLRRSGIDNPAIGRGLRLHPASAIAGIFPQRIE
                     PWTGALQTRYSDQFADSGDGYGVKFETAPVHFALPASGFGWESAVRHREDIGRLANLS
                     IIGTLLRDRDPGHVAVGRDGRPRVHYELSHHDVANVRRGLLGAAQVLAAAGATELFSL
                     HTPPARVTTAQPGWLSTFGDAMDHRGYTKCRMSYITFHQMASCAMGADRRQSVVGETG
                     ESHDVAGLYVADASAFPTSSGVNPMITIMAIADHVARGIIERR"
     gene            14009..15757
                     /locus_tag="AC16JC_05880"
     CDS             14009..15757
                     /locus_tag="AC16JC_05880"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009099 - L-valine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HIR5164260.1"
                     /translation="MRAHLGVMKLTGGEIVARALADEGIRDAFGIPGTHNIELYDALA
                     ASGAVRPVLVTDEQSASFMADGYWRASGRLACVNLVPGAGLTHALSGIAEAFLDNVPL
                     LVLGCGIRRDFPKAYQLHDIDQLAVARPVTKAAYRIERGEDLYPEIRRACALARAGVP
                     GPVMVEVPVNLYLTRHDVAPESWGASPAAAPPGGTAAQLERVAAALNDPAARPLLYLG
                     LGAAGAGADLVTLAERLEAPVATTFQGKGVFPESHPLFLWNGFGAAAAPFARAVAERC
                     RGTLAIGCRFGEVGTGSYGLEPPRPLLHVDIDPAVPGRNYETAVAVAADAAAFVRALL
                     PRLAGRPRDEQLRAAIRDGHARVWRDWLAHGAADRVSPPRLLRALQEAFGAETVFTTD
                     SGNGTFLAMECLRLERPGRFLAPVDYSCMGYAVPAALGAKLARPECPVVALAGDGALL
                     MTGLELLTAAQLGIAVAVFVLRDRELAQIAQFQATALSRKTASELPDYDAGALAGALG
                     VEALRLARDPDIPGVLAAVREKVAAGRPVLVDVAIDYSHKTFFTAGVVKTNLLRFSWP
                     ERLRFIGRALSRKLLG"
     gene            complement(15754..16674)
                     /locus_tag="AC16JC_05885"
     CDS             complement(15754..16674)
                     /locus_tag="AC16JC_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013761.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR5164261.1"
                     /translation="MSIARSLLLRASRSPWLAEQFRRREFARRAVRRFMPGESLLDAL
                     DAAAELAKASLGTVLTQLGEQVRNAAEAEDVRDHYLNVFDQVRDRFLPAQVSVKLTHL
                     GLDVDRDACLRHVRTLAEKARGEGCMLWIDMEESRYVDATLDLYRRVREAGPGVGVCL
                     QAYLRRTPQDLAALLPLSPAIRLVKGAYNEPGNVALPRKKDVDAQYSTLAVQLLDAAA
                     AGHATTVFGTHDLPLIERIREQAAARKADGRYEVHMLYGIRSDAQRRLAGQGVKVRVL
                     ISYGSHWFAWYMRRLAERPANVWFVARSLF"
     gene            complement(16768..>17165)
                     /locus_tag="AC16JC_05890"
     CDS             complement(16768..>17165)
                     /locus_tag="AC16JC_05890"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR5164262.1"
                     /translation="TRVLEWDTLVPNGRVTASVAQGCVTIGGDVDWDHQRVAAEHAVS
                     PLLGVRAVDNQITVRPKWMTGELQTSVTAALRHRHDLHTQHVHVETVRGMVVLQGYVP
                     SLAERSAIERVAWQAPGVLGVVNELSIER"
BASE COUNT         2507 a         6088 c         6153 g         2417 t
ORIGIN      
        1 tcggtgaatc tgcagcgggt cgaaacgctc aaagaggatc cgaacatccc gccggtggac
       61 ctgaagtcgc tggcgcagga gttcgtgcag cagatcagtt cgtgggcgaa cgccggcgag
      121 taagcgaacg cggcgttgcc cctcgacggg cggtcccggc agtgcggggc cgcccgtttc
      181 acttggtgtc ggcttcgagc tcggcttccg cgtccacccg ggcggcgagt tcgcgcaccg
      241 cctcgagatc ctccgtcagt ccatcggtcg acccgatccc cgcccgcagg cgcagcaggt
      301 cgagccgcag gctctccatc gcgctcaccg tcgtgccgag acgctccgcc gcggtgcggc
      361 gtgcggcctc gagatcgtgg gccagagcgt cgcgccggga agatgcggca gcgcccacct
      421 gggccgccgc ctgatctagc tcgcccatgc ggcgccgcag cacggcggca tcggcctcga
      481 ggcgagtgag cagctgctcc acgccgacca ggcggcggtc ggggtcgggg agcgcgtgca
      541 ggatgtcgcg cgcggcgtcg gcgatgagga cttccgtgtg ttgatccgcc ggcgcgacca
      601 ccgccggcgt gcgcagtctc cggcccagcc gggccagcac gaagaagccc ttcattttga
      661 cccgcatgac gagccgactg aacaagcccg gcttcccgtc gcgcggcgcc tcgagccggc
      721 tgccgtacca gcggccccat gcgaccgggg cgatgaggcc tacgagctcc acgagccagg
      781 cgagcggtcc ctcgagcgag gacaggccag cctccttggg gcccagccag taggcgacca
      841 gcgccgcgaa tccgacgagg aagatcgctg tggcgcccag ccgtcccgga cggggctggc
      901 gcacggtgga tcccgcggca cgcgcctcgc gctcgatggc cgcggcgagg tcccgctctc
      961 cccacccttc ggcgacggct tcgcggatga gacggaacat cgacatgccg cgcagcgccg
     1021 tgaggctgac cagcacgagc gcaatggcga tcgtgtacac ggtgcgaacg ccaaagaaat
     1081 cgaattcgtt gctgaacagg aacttcgaga cgatcgcgac cgcggcaagc gtaccgtacc
     1141 ccgcgagatc cactgcgaag gacgagatct cccgcgtgat ccgctgcaac gtggccggga
     1201 ccgccgtgcc ctcccggacg gccgccacgg cgtcggcgag gtcgcccgcc gtcgtgaacc
     1261 ggtccgcggg agtcttggcg agacagcgat cgatcgccgc ggcgagggcg ggcggcaggc
     1321 cggcgcgcac ggaggtcacc gggggcgccg gcaacgtctc gcgctgcacc atcaggccat
     1381 gggcatcagc ggcgtcgaag gggagccgcc ccgtcagcgc gtagaacgcc gtcacgccga
     1441 gtgagtacag gtcgctccgc ccatccacca cctcgcccgc ggcctgctcg ggcgacatga
     1501 agcgtgccgt ccccaggaca cggcccgatc ccgtgggcgg tgtctccgct ccgagacgcg
     1561 cgatgccgaa gtcggtgacg agggcccggc cggtgccccg ctccagcagg atgttctccg
     1621 gtttgatgtc acgatgcacc acgccgtgct gatgcgcgtg ggcgagtgcc caggcgactt
     1681 cctgcaccat gcgggtcgcg gccgcgggat cgagtggtcc ctgggtgcgc acgcgctccg
     1741 ccagcgtcat gccggcgatg tagcccatga cgaagaacac gagatcgtcg tgctgctcga
     1801 cggcgtggat cggaacgatg tgggggtggg cgaggcgggc cgcggtgcgc gcctcctgca
     1861 ggaagcgctc gcgctgctgc ggcgtcgcca gcgccggggg aaggagcttg atggcgacgg
     1921 gccggtcgag cgccacgtcc cgtgccagga acaccacacc catcccgccg cgccccagct
     1981 tgcgctcgag gacgtagcgc ccacgcaagg cggcttcgag cgcgacgaac tccggggaag
     2041 acaagccctg ggtcatgacc ggcccaatgt agcgcatgag atggggtggg gacacgacgc
     2101 ccggggagaa gccggagttt catgggacgg atcgcatccc cggtcgtata ttcgcccccc
     2161 gtggaccgac tggaagggct cgccaaactc acgggccgcg aacgctacgt cgacgacctg
     2221 ccgctcgacg attttctctg gggtgcgacg gtgcgcagcc cggccccacg cggccggatc
     2281 acggccgtgc gatttggccc cggcgtggac tggtcgcagt tcaccctcgt ggaccatcgc
     2341 gatctccccg ggcccaacgt gatcgcgctg atcgagaacg atcagccggt gctggcggcg
     2401 gggagcgtgc gccacgtgca cgagccggta ctgctgctcg cccaccctga ccgggcggcg
     2461 ctgcgacggg cggtcgccgc tgtgaccatc caggtcgcgc ccgagcccgc cgcgctcgac
     2521 ttccgcctcc cgcccgcccc cgagacgatc cagtacggct ccgacaacat tctgaagcac
     2581 ctatcaatct ccaagggaga cgtggagcgc gccctcgccg cggcgccgat cgtggtggag
     2641 gggacctacg agaccggcgc gcaggagcac gtgtatctcg agccgcaggg gatgatcgcg
     2701 caggtcgatg gagatgtcct cgtgctgcgc ggctcgatgc agtgcccgta ctacgtcctc
     2761 aaggcgctgc gcacggccct cgcccgtgac gaccggcacc tgcgggtggt gcagaccccc
     2821 accgggggcg gcttcggcgg caaggaggag tatccctcga ccatcgccct ccacgccgcg
     2881 ctgctcgcgt tgaagagcgg caggccggtg aaaatgatct acgaccgcac cgaagacatg
     2941 gcggccacca ccaagcggca tcccgcgctg gtgcgccacc gcaccggcgt cgccacggat
     3001 ggtcgcctgc tcgcacagga catcgacgtg atcatggacg gtggcgccta cgtcacgctc
     3061 tcgccggtcg tgctgtcgcg cggcatcatc cacgccgccg ggccgtacgc gtgcgaccac
     3121 gtccgtatcg acggccgggc catattcacg aatgcggtgc cgttcggcgc gttccgcggc
     3181 ttcggcgcgc cgcagacgca cttcgccaac gagcggcaca tggaccggat cgcggcccgc
     3241 ctgggtctcg atcccgccgc gctgcggcgc cgcaacctgc tgcgcgatgg gcaatccacg
     3301 gcgacgggcc aggtaattca ggacgggacc gaccgcgtgg cgctgctcga tcgtgccctc
     3361 tcgctcgccg attacgacgc gaagcggacc gcccacgcgg cgcacaatgc cgccagcgcc
     3421 accacgcggc gcggcatcgg catcgccacg ttccaccatg gggcgggatt cacggggagc
     3481 ggcgaggtgg cgctggcctc gcggctgcac gtagcgggac tgcccgacgg gcgcgtcgag
     3541 gtgcgctccg ccaacatcga gatggggcag gggacgctca cggtgttcac ggcgctcgcg
     3601 gccgagcgac tggggctcga gctctccgag gtggtgatcg ccgaggccga cacggcacgt
     3661 gtgccgaaca gcggcccgac ggtcgcctcg cgtaccgcga tggtggtggg gcacttggtc
     3721 gagcaggcct gtgatgcgct gcgggcgcgc gtgggcctgg acgccggcgc cacgggacaa
     3781 accgtgcgtg acgcgctgcg ccggtggcac gagcgtcacc cgggcgagga cctcgttgga
     3841 gaggccgtgt accagcgtcc tccagggatt tcctgggacg agacgaccta tcggggcgac
     3901 gcctacgcca cgttcggctg gggcgcgtac gtcgccgaag tcgaggttga cctacggacc
     3961 tggcagaccc gggtgattgg cttcgtggcg ctgcaggaag tcgggcacgt gctgaacgac
     4021 tgcctggcgc gcgggcagat ccagggcggc gtggcgcagg cgatcggctg ggcgctgttc
     4081 gaggagtgcc gctggcacga cggcgcgatg gccaacgccc agctcaccaa ctacctcatt
     4141 cccacgagcg acgacctgcc gccgatccgc gtggcgttcg tcgagaatcc gtatccgctg
     4201 ggcgcccagg gggccaaggg gctcggtgag ctgcccatgg acggcccggc ggccgcgatc
     4261 ctgaacgccg tcgcggccgc caccggcgcg gacccgtgcg cgattccgct gacgccggag
     4321 cgactgatga aaattgcgga ttggggattg cggattgcgg attccaaagg tgagggcaca
     4381 gtcccaggag cggtcaatcc gcaagccgca atccgcaatc cgcaatctcg atcaccttca
     4441 ctctgaacgg ctccacgatc tctgtcgagg ccgatcccac cgaccgcctc ctcgacacgc
     4501 tgcgctatcg cctgggcctc accggcacca aggagggatg cggcgagggg gaatgcggcg
     4561 cctgcaccgt gtacctggat ggccttccgg tgaactcctg tctcgtgccg atctaccagg
     4621 tccgcgggtg ccgggtcgag accgtggagt cgctcgatcc cgcggtgttg cggccgctgc
     4681 tcgcgagtgg cgccacgcag tgcggcgcct gcaccccggg cgtcgcgatg acggcctgct
     4741 ggattcgcga gcaccgggag ctgctggcga gccattcggt ccgcgagctg ctcgccggca
     4801 atctgtgccg ctgcacgggc tacgacggca tcgtggacgg catccgggcg tccctcgagg
     4861 cgcgcgcgtg aagctgctgc tgccggagtc gcgcgaccag gcgctcgccc tgctcaccgc
     4921 gacgcccgct gcggtcccga tggccggcgg cacggatctg ctggtccact ggccgctgcg
     4981 caccgcggaa cacgaccgca cctatctgga tctctcgcca ctgcgcgatc tcaagggcat
     5041 gcgctgggag gacggagcgc tggtgctggg gggcctcgcc acctactggg acgtgatccg
     5101 cgatccgcgt accgcacagg agctgccgct gctcgtcgaa gcggcccgac aggtgggcgc
     5161 ggtgcagatt caggcgcgcg gcacctgggc cggcaacatc gtgaacgcgt cgccggcggc
     5221 ggatggcgtg ccggtgctca tggcgtacga cgcgatcgtg gtgctcgagt cggtgcgcgg
     5281 ccgggcggag gtgccgcttg cggagctcta cacgggctac aagcagctgc gccgggcgcc
     5341 ggacgagctg gtggtcgcga tccggatccc tcgccgggtc tacgcgctgc agcgcttctt
     5401 caaggtcggc gctcggcgcg cgcaggcgat cgccaaggtc ggtctggcgg tgacccgatc
     5461 gtcggcggga tggcgtgtgg tggccgcgag catggcgccg acgatccgtc gctgcccgtc
     5521 gctcgaacgc ctgctggagg aaggcgccga catccgttcg ccggacgatc tgcttcccgt
     5581 cctcgcccag gatgtgtcgc cgatcgacga catccgctcg agcgcgacat accggcggca
     5641 ggtgatggcg cgcgtgctgt acgatgcggt gcgcgggtca tgaagggggt ctggctcgag
     5701 gatcgccggc tgcggctgcg ggacgatctc cctgttccgg agcctcccgc cggcgaagcg
     5761 ctggtccgcg tgctggccgc cgggatctgc aacaccgacc tggagctggt ccgcggctac
     5821 tacccctaca ccggggtgcc ggggcacgag ttcgcgggcg aggtcatgtc ggacggcgcg
     5881 ctcgccggca agcgggtcgt gggcgagatc aacgcggtgt gtggtgactg ccgggcgtgc
     5941 cgggcgggcc ggcgcagcca ctgcgagcgg cgtacggtac tgggcatcgt caaccgtcac
     6001 ggcgccttcg cggaatacct gacattgccc gtcgagaacc tgcacgtgat ccccgacggt
     6061 gtcacgacgg aggtcgcggt gttcaccgag ccgctcgccg cggcattgga gatccaggag
     6121 caggtggcga tcgctcccac catgcgggtg ctggtcgtcg gagacgggaa gctgggccag
     6181 ctcgtggccc agaccctggc gctcaccgga tgcgatctcg tcgtatcggg ccgtcacgag
     6241 gccaagctcg atttgctgcg ccggcgcggg atcgcgacgg tgaccccggg cggcatcgcg
     6301 gctggggcat tcgacgtggc ggtcgagtgc accgggaatc ccgagggatt cgcagtggcg
     6361 cagcgcgcgc tgcggccacg cggcacgctg gtaatgaaga gcacctacgc cgacaagctc
     6421 acgctcgacg cctcggcggt cgtggtgcac gagctgacgc tgatcggctc gcgctgcggt
     6481 cccttcccga aggcgctcgc cctgctcgcc gcgggccagg tggacgtgtt acccctcgtc
     6541 tcagcgcgtt atcccttgga tcgggcgctc gacgcgttcg aacacgcgca gcgaccgggg
     6601 acactgaagg tcttgctcgg gagatgagac tcagcggagc gacgcgcccg cgcgtaccca
     6661 gcgcccgagc agcgcacgct cttcggcggt gattcccgtg acgttgccga gtggcatcgt
     6721 ctgctggagg acggcgcgtt cgacgatgcg tgcggcccgg gcccggatct gctcgggcgt
     6781 gtcgaggatc acgccggcgg gcgcggccgt gatgcctggg taggtgggcg ccgtggagtg
     6841 gcacggtgca cagcgccgcg cgatcacgac gcgcacttct gcgaaggaaa cggagcccag
     6901 agaccccgct ggagtgccac cgccgggacg tggcgccgtg acgatcgcca agatcatgag
     6961 gccggccacc gccgccggca ggagccaggc gttgcggcgt cctgcgttgc gcaggttgaa
     7021 ccagtggcgc accagcacgc cgatcacgaa caacacgccg agaatcgccc acccccagcc
     7081 gctgccatag gtcgccgggt agtggttgct caccatcacg aacagcaccg gcagggtaag
     7141 atagttgttg tgcagcgagc ggagagctcc ctggcggccg cgggccgcgt ccggcgcggt
     7201 ccccgcggac agcgcggcga ccatgtcgcg ctgcgccggg atgatcaccc ggaacacgtt
     7261 cgcggccatg atcgtgccca gtgccgcgcc gacgtggaga taggcggcgc gggcactgaa
     7321 gacctttccc agcccccacg cgagcgcggt cacgaggacg agtcccagca ccgccagggc
     7381 ggctggccgg cccgcgatcg gcgatcggca cagcagatcg tagacgagcc agcaaacgac
     7441 gagcgtcccg acgcccagca tcacggccgc catcggaccg atggacgaga cggcgggatc
     7501 gagcaggtac tcgcgcgctc cggcataata gagcagcacg aggagcgaga ggccgctcca
     7561 ccacgtggca taggcctccc acttgaacca gtgcagcgtc cgcggcagcc gcgcgggggc
     7621 gaccgagtac ttcactaccc gatagaaccc gccgccgtgc accgaccaca gctccccgcc
     7681 cacacccggc tctggcgttt cgagcggcgc gatgcgccag ttcagccagt tgaaatagaa
     7741 cgacgtgccg atccagctga ccccggtgat cacgtgcgcc cagcgcaaca cgaggctcag
     7801 ccactgcacc aggtgggcgc tcacccgtca actgccgcgg tacgtcgagt agccgaacgg
     7861 gctcacgagc aggggcacgt ggtagtgccc gtcgtcgtga ggggtgaata cgacctcgac
     7921 gtagggatag aacgcggcct caccgcgcgt cgcgaagtac tcccccacct cgaagcgcag
     7981 gcggaaggtg gtgccggccg ccgcgggagc ggtcgccggc agcagggtcg ggatccgccc
     8041 gtcggcgtcg gtcgtcccgc gcgccagctc ggcccagccc cgcgggccct gggccgagag
     8101 cgtcaccgcc accccggggg cggggcggcc gcgcgccgtg tccagcacgt gcgtcgatag
     8161 cctcatgcgg tcactcccct cccagtttct ccagtcgcag agccgtgatc ttcgcctgct
     8221 ccccggccgc ggtgcgcagc tcctgctccg ggtccgccac gagccggcgg cgcaggtccg
     8281 ccagcagatc cgcggcgcca cggcctgtcg cgcacacgag gtagacgtgg ccgaagcgcg
     8341 cctcgtaggc gcggttcccc tgctcgagcg cccggcggac gtccgaggcg gcctggtcca
     8401 cactactctg ctcgccgcgc gaccaggcgt caccgctgcg ctcgccgatg cgcggatgcg
     8461 ccgcgaacgc ctcgagccag tccgcccgcg ccagggtcca ccactcgcgc tccgccgcct
     8521 cgagcagcgc ggcgtcgctc gcgtacggga ggccggcgag catccgctcc acccagcgcc
     8581 gcgacccgca gcagcgccgc aactcggtct cggcctcgga gcggggaagg gcgttcaagt
     8641 acgccgccac ccttccccct tccctcttcc ccctttcccc gtctttcaga cgcgtctccc
     8701 atagaggcga aggcgcgcca cgcccccgtc cggaaagatg ctgaaccgca catgcgtcac
     8761 cggccccgaa cggccgacgg cgcggaagcg gtgctgcgcg tccgccttca gctgcgtgcg
     8821 cggcaccagc tcgcgccagg gggcgccctc gtccggcacg gcgttcgctt cgagtccggg
     8881 gagatcgcag ccctcgaggc ggcagctctc ggggaagttg cccttgaaat gggtggtgtc
     8941 cacccgcacc gcgtcgatcg tgccgcgggc gccgagtcgc aggatgaccc agtcgtgacc
     9001 gggcccacgc cgccggcgcg tctcccagcc gtcgctcatg tcctcggggc cccccggcat
     9061 gagcaggttg agcggatcgc tgaagaaccg gtcgctcgcg gccagcggtg cgccgccgtt
     9121 gcggagcgcc gcgagatcga ggggccgggt cttcttctcg agcgctgccc agtcgggccg
     9181 cgcctcgccc cacaggcgca gtcgcgcgac gccgccgtcg gggtagatgg tcagccgcac
     9241 gtgcgtgatg cgcgactcgc ccgtgaccgt gaaatcgttg agcgcgtcgc ccgcgagcgg
     9301 tgcgcgcggc aggagcgtgg cccacgccac agcgccgccg ccccggcgct tgaaggtcgc
     9361 gtcgtccttc cccttcagcg tcgccgcgtc gagcgagcag gcctctgggt gattgccccg
     9421 gaagtggcgc gtgtccaccg tgacggcgcg gaccacgccg gggacgccga gccgcacgat
     9481 gcaccagtcg gaaccgggcg tgcgtcgccg gcgggtctcc cacccgtcca tccacttgcc
     9541 gcggctcgtg tacttgcccg cggcaaatac ggggggcccg gggttcagca ggttggactt
     9601 gggcgcgaag aactcgtcgt tggcgagcac ggcgcgcccg ccgacgcggg ctgccgccag
     9661 atcgatcagt cctccattca cgaacacctc cctgactggc gcaaccggcc ctccaccgcg
     9721 catcttgagt cgtcacccga gggacgaacg gcatggcgga gaagtcctac agtggacccg
     9781 aacggcgcaa agcgaagcgg cgcaagcgcg ccgatcgccg gaacgtgttc gacaggcggc
     9841 gctggaagga gcggcgccgc acctggggcc ggacgtctac cgagcggcgc gtcgataagg
     9901 accggcgctc cctcgttccc cggcgaggcg gcgacgagcg gcgcaccggg cgcgatcgcc
     9961 gcggctccga cgagtagtca ggcccgcccc gaccggtcct cccggcgcag ccacgtcccg
    10021 cccggtggag cgggaaagcg gccctcctcg tacacgcaga tcccccgcag gaacgttcgc
    10081 agcaccttgc cacggagcgc cagacccgcg taaggcgtaa ggggatggcg ctggtgcagc
    10141 cgctcggcgt ccactgtcca cccgtgcccc gggtcgaaca ccaccagatc cgcgtcgtat
    10201 cccggcgcga tgcgaccctt gcgagtcagc ccggcgaggt gcgccggccc ctgtgacagc
    10261 caccgcgcca gcgccgggag gccgtgaccg cgcgcgcgcg ccccggtcca gatcgccgcc
    10321 agggacagct cgagcgacgc gattccgccc cacgccgcca ggaagtctcc cgtctcgagg
    10381 tgcttcagcg cgggcggtgc gggggagtgg tcgctcgcca cgagatcgat cgctccgtgc
    10441 gcgaggccgt gccacaaccg ctcccggttc tcgcgctccc gcaccggcgg tgcgcacttg
    10501 aaggcggtct gcccgtcggg aatatcctcg gcggcgaagc tgaggtaatg cgggcaggtt
    10561 tcgaccgtga cgcgcgcgcc ggcccggcgg gcgttggcga tccggtccag ggccgcggcg
    10621 cacgcgaggt gcacgatgtg cacccggcat cccgtttcca gcgacagcag caacagcagg
    10681 tcgatcgctt cgagctcggc gccgcggggc cgcgccgcga gccaggtggc gtagcgtcgc
    10741 gggtcggcac cggtcaggct cgcctccgcc tcggagatgg gcccgggcag ctcggcgtgc
    10801 gtcagcaaca cctggtcgag ctcggcgagc cggtgcatcg tggcgaccag atcgctctcg
    10861 gtcaggtggg agaactcctc cacccccgaa gggacgagaa agcatttgaa gcccggcacg
    10921 cccgcccgcg agagcggctc gaggtcgtcc aggttctccg gaacggcgcc cccccagaat
    10981 gcatagtcca ccatcgcctg acctgccgct gcgcgggcct tggtctcgag cgccgctacg
    11041 gtggtcgtgg ggggcacgct gttcagcggc atgtccacga tcgtcgtcac accgcccgcc
    11101 gcggcggcgc gcgtcgcact ggcgaacccc tcccactccg tgcggccggg ctcgttcacg
    11161 tgcacgtggg agtccaccag acccgggagg agtgcggcgg cccccacgtc gtgggtctcg
    11221 gccgcggctg gcgcctctcc gggcggcacg acggcgatga tgcggccctg cgctacgacc
    11281 accgcgccgg gccgctcgcc ttcgggcgtc acgacccggc cgctcgaaat gacgaggtcg
    11341 ggtttcaccc gccccggatc aggcgcgtga gcgccggctc gattcccatg agcctcgcgt
    11401 acagcgcgac ctggccacgg tggtactcct catgcgcgat gccgtggttc caccacgcga
    11461 gcctcgtacc cgggagtccg tcgaaccggg tgatcagctg cagcagcgac agctcacccg
    11521 cccggcggaa ccgccgctcg ccgtccttga gctgcgaggt gagcaaccgg agcagcatcg
    11581 gcttcgtcct ggcgcgccgg gccgccctgg catacagccc caacagcttc ggccacggcg
    11641 cgcggtggaa attcgtatcg ggccgcgtca gctccccggt catcatcatc gcgacctcga
    11701 gaatgtgctg caccagctcg gcgacgctgc gcgactgcgg ggtgggacgg aaatcgaagc
    11761 ggctcgcggg gatgttgcgc acctcggcga tcagtccctc gcgcaccccc tgccaggcct
    11821 cgagcgtttc ttcgagcatg gtgttgtacg gcggcatgcg ctcctcctcg atgctgaaag
    11881 atcaccgaag cagccgatag ccgatagctg atagccgata gccgctttag cgccgctcga
    11941 tgatcccccg cgccacgtgg tcggcgatcg ccatgatcgt gatcatcgga ttgacgccgc
    12001 tcgaggtggg gaaggcgctg gcgtcggcca cgtacagccc cgcgacatcg tgcgactcgc
    12061 ccgtttcacc caccaccgat tggcgccggt ccgcgcccat ggcgcagctc gccatctggt
    12121 ggaaggtgat gtaggacatg cggcacttgg tgtagccgcg gtggtccatg gcgtcgccga
    12181 aggtggacag ccacccgggc tgggcggtcg tgacccgggc cgggggggtg tgcagcgaga
    12241 acagctcggt ggctccggcc gcggccagga cctgggccgc gcccaggagg ccgcggcgca
    12301 cgttggccac gtcgtggtgc gacagctcgt agtgcacccg gggccgcccg tcgcggccga
    12361 ccgccacgtg ccctggatcg cggtcccgca gcagggtccc gatgatgctc aggttggcca
    12421 gtcggccgat gtcctcgcga tggcggacgg cgctctccca cccgaacccg ctggccggca
    12481 atgcgaaatg aaccggggcg gtttcgaact tgaccccgta cccgtcgccc gagtccgcga
    12541 actggtccga atagcgcgtc tgcagcgctc cggtccacgg ctcgatgcgc tgcgggaaga
    12601 tcccggcgat cgcgctggcc ggatggagcc gcagcccccg gccgatcgcg ggattgtcga
    12661 tgccggagcg tcgcagcaac gccggggtgt tgatcgatcc gcaggcggcg acgacgatgg
    12721 gcgcgcgcac ggtcagccga tggcgcgcct cgtcgggtcc ggcgaccgtg ccctcgacgc
    12781 ccccggcccg gccggtcgcg acgaggacgc ggtcgacgtc gcaatggggc acgaggtggg
    12841 cgccgtgccg cgccgccatg gcgaggtagg tgcgggcggt cgagttcttg gccccgtggc
    12901 ggcagccgta cccacaatac ccgcactcga cgccctcgcg gcaatcggtg acgttgcggg
    12961 gaatcaggtc cacgtgccag ccgagcatct tgcagccgcg ctccagcagc tggtcgcgca
    13021 ctcccggcgt ggtatagcgc gtgccgacgt cgacctggat cgcgacacga tcgaacgagc
    13081 gctggaagcg cggggaggtg aacagcgtga gtccggagcg gcggtcccac tccgcccgcg
    13141 tcgtgggcgg gagcgggaag ctggtcgtgt agttgatcgc ggtaccgccg ccgaggcagc
    13201 tgccggccag tatcgggagc gagccgctct gggtcatgat gaggccgccg tcgaggtagt
    13261 accgcgacag catgtcgcct tcgacctggg tgaagtcgcg cgagcccggg ttgcccccct
    13321 tctcgaggac cacgaccccc atgccggcct cggcgaggag gcccgccatc actccgccgc
    13381 ccgctcccga tcccacgacg accgcgtcgc actcgagcac ggtatcgcga tcgaccgaca
    13441 gggtcggcag cggctcgagg cacttcgcgg gttggggcag gggtccgggg tagccgttcg
    13501 cgtcccaggc cgggtggcgc gtgcccgcgc tcgggggcca gcagtagtgc gccacgtgca
    13561 ccaggcgttt gagggcctgg aaaccggcgc gccgcagcgc gatgtgcgag tgcgcgaagc
    13621 cgcgcagcac cgcctcgcgc gccgggggct gcatcgcagt gaagcgggcg aaccggccgg
    13681 tgaagagcag attcacgaac gggttgtcga gcgtccgcag gagcagccgc aggcggtcgc
    13741 ggtcacccgg atcggggatc gacccgatca gccgcacggc gcgatccgcc gtgccgagcg
    13801 ctcgggcgcc ggccgcgaag aaccctgccg gatcgtcggc gcgcgaaatc gacggcagca
    13861 gcgtctcgca caccgcatcg agcaccgcgt gctcccccgg cgacaggacg ccgttcgctt
    13921 ccaccatcat ggccctcctg tggcactaag cccggaataa gtcaccgcgc accttgaggc
    13981 tcgatggaat ccagttgaag agagcttcat gagggcgcat cttggcgtca tgaagctcac
    14041 gggtggagag atcgtggcgc gggcgctggc ggacgagggc attcgcgacg cgttcggcat
    14101 cccgggcacg cacaacatcg agctgtacga cgccctcgcg gcttccggcg ccgtccgccc
    14161 ggtgctcgtg accgacgagc agagcgcctc gttcatggcc gacggatact ggcgggcctc
    14221 ggggcggctc gcgtgcgtga acctcgtgcc gggggccggc ctgacgcacg ccctgtcggg
    14281 catcgcggag gcgttcctgg acaacgtgcc gctgctcgtc ctggggtgcg gcatccggcg
    14341 cgactttccg aaagcgtacc agctgcacga catcgatcag ctcgccgtgg cgcggcccgt
    14401 caccaaggcg gcctaccgta tcgagcgggg ggaggacctc tatcccgaga tccgtcgcgc
    14461 ctgcgcgttg gcgcgcgcgg gcgtgccggg cccggtgatg gtcgaggtcc ccgtgaacct
    14521 gtatctcacc cggcacgacg tggccccgga gagctggggc gcgtctccgg ccgcggcgcc
    14581 gcccggcggc accgcggcgc agctcgagcg cgtggcggcg gcgttgaacg atcctgccgc
    14641 acgcccgctg ctctacctgg gactcggggc cgccggcgct ggagccgacc tggtgacgct
    14701 ggccgagcgt ctcgaggccc cggtggccac gaccttccag gggaagggcg tcttccccga
    14761 atcgcaccca ctgttcctgt ggaacggctt cggcgccgcc gccgcgccct tcgcgcgtgc
    14821 ggtggcggag cggtgccggg ggacgctcgc gatcgggtgc cgcttcggcg aggtggggac
    14881 ggggagctat ggactcgagc cgccgcgtcc gctgctgcac gtggacatcg accccgccgt
    14941 cccgggccgc aactacgaga ccgccgtcgc ggtcgccgcc gatgcggcgg cgttcgtgcg
    15001 cgccctgctg ccgcggcttg ccggacgccc gcgggacgaa cagctccgtg cagcgatccg
    15061 cgatgggcac gcgcgggtgt ggcgtgattg gctggcgcac ggcgccgccg accgcgtgag
    15121 cccgccgcgc cttctgcggg cgctccagga ggcgttcgga gcggagacgg tcttcaccac
    15181 cgacagcggc aacggaacgt tcctcgcgat ggagtgcctg cgcctcgagc gtccgggccg
    15241 gttcctggcg ccggtggact actcgtgcat gggctacgcg gtacccgcag cgctcggggc
    15301 gaagctcgcg cggccggagt gtcccgtcgt ggcgctcgcg ggcgacgggg cgttgctcat
    15361 gacgggtctc gagctgctca ccgccgcgca gctgggcatc gccgtggcgg tcttcgtgct
    15421 gcgcgatcgg gagctggcac agatcgcgca gttccaggcg accgcgctct cccgcaagac
    15481 ggcgagcgag ctgcccgact acgacgcggg ggcgctcgcc ggtgcgctgg gcgtcgaggc
    15541 cctgcggctg gcgcgcgacc cggacatccc cggggtcctg gccgcggtgc gggagaaggt
    15601 cgcggccggc cggccggtgc tggtagacgt ggcgatcgac tactcgcaca aaaccttctt
    15661 cacggcgggc gtggtgaaga cgaacttgct gcggttctcc tggcccgagc gcctgcggtt
    15721 catcggccgg gcgctgagcc gcaagctgct cggctagaac aggctccgcg ccacgaacca
    15781 cacgttcgcc ggccgctcgg cgagccgccg catgtaccag gcgaaccagt gtgagccgta
    15841 gctgatcagc acccgcacct tcaccccctg gccggcgagc cggcgctgcg cgtcgctgcg
    15901 gatgccgtac agcatgtgca cctcgtagcg gccgtcggcc ttccgcgcag ccgcctgttc
    15961 ccggatgcgc tcgatcagcg ggaggtcgtg cgtgccgaag acggtggtcg cgtgaccggc
    16021 ggccgcggcg tccagcagct ggacggcgag cgtggaatac tgcgcatcca cgtccttctt
    16081 gcgcggcaac gccacgttgc ccggctcgtt gtaggcgccc tttacgaggc ggatcgcggg
    16141 cgagagcggg agcagggccg cgaggtcctg cggcgtgcgc cgcaggtacg cctggagaca
    16201 cacgccgacg ccgggacctg cttcccgcac gcggcggtag aggtcgagcg tggcgtcgac
    16261 atagcgggac tcctccatgt cgatccacag catgcagccc tctccccggg ctttctccgc
    16321 gagcgtccgc acgtggcgca ggcaggcgtc acggtcgacg tcgagaccga ggtgcgtcag
    16381 cttcaccgac acctgcgccg gaagaaaccg gtcgcgcacc tggtcgaaca cgttgaggta
    16441 gtggtcgcgc acgtcctcgg cctcggcggc gttgcgcacc tgctctccca gctgcgtcag
    16501 gacggttccc agcgacgcct tggcgagctc ggcggcggcg tcgagcgcgt ccagcaggct
    16561 ttcgcccggc atgaagcggc gtacggcgcg acgcgcgaac tcgcgccggc ggaactgctc
    16621 ggcgagccac gggctgcggg aggcgcgcag caggagtgaa cgggcgatgg acatgaagcg
    16681 gacaagatag tcgccgggct gccgaaacgc ccgccgggcc ccagcgaccc ggggcccggc
    16741 gagtgaccta ctccgtaccc gctttgctca gcgctcgatg gacagctcgt tcaccacgcc
    16801 cagcacaccg ggcgcctgcc acgcgacgcg ctcgatcgcg gaacgctcgg cgagggacgg
    16861 gacatagccc tgcagcacga ccatgccgcg taccgtttcg acgtgcacgt gctgcgtgtg
    16921 caggtcgtgg cggtgccgca acgcggccgt cacggaggtc tggagctcgc cggtcatcca
    16981 cttcgggcgc acggtgatct gattgtcgac ggcccggacg cccaggagcg gcgacaccgc
    17041 atgctcggcc gccacgcgct gatgatccca gtcgacgtcg ccaccgatcg tcacgcagcc
    17101 ttgcgcgacg gacgccgtca cgcggccgtt gggaaccagc gtgtcccact ccagcacgcg
    17161 tgtga
//
LOCUS       DBNGZW010000247        10565 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1719521, whole genome shotgun sequence.
ACCESSION   DBNGZW010000247 DBNGZW010000000
VERSION     DBNGZW010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 10565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10565
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1719521"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(387..1112)
                     /locus_tag="AC16JC_05895"
     CDS             complement(387..1112)
                     /locus_tag="AC16JC_05895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164263.1"
                     /translation="MFLTIVCCGTALLGVSPTSDTTLTLQGVVAVSGETPGGAGRAVM
                     VLTEPILIGSTQMWILPLGGDAARWRNWDSRYIEARGELGSQPPQGIRFTPVQIRDVE
                     PDSGSSGEVVSPSHHSNVRLAVLPHRFTAVPGDSTGGITPLIYYKITVQGQTNLTFEF
                     SNNEFICVSVRAPGERDPGWRNAWLVPQERPPYVIRVGPVFRVLIPIPADVVVRPGRY
                     TVQATVCGNPEFNLTTTIDVSPT"
     gene            1245..4217
                     /gene="fdhF"
                     /locus_tag="AC16JC_05900"
     CDS             1245..4217
                     /gene="fdhF"
                     /locus_tag="AC16JC_05900"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HIR5164264.1"
                     /translation="MMSITINGRPLSAAPEQTILDAARAAGIDIPTLCWYPTLPIVGN
                     CRICLVSVEGQPKLLPACATKVADGMVVTTESHAAVENRKAVLHFLLERYPGEHLRNG
                     DRADPRNEFEQYVVRYGVTPSPAAGRDLPLRSGDERPGDPMIRHDMTTCILCTRCVRA
                     CEDLQVVGVLDVAQRGEHAEIIVGADGDPDRAGCTWCGECVRVCPTGAIFEVMPRERF
                     RPEQVRRPETIVRAVCPYCGVGCQVDLHTADDKIVRVTSPDIELDTPNQGSTCVKGRF
                     GYDFPQHRDRLTRPLIRRGWQREADGRWVYRGAGGADRRAGPWRTIEGEGQQSKPGTP
                     PRKTGKSVRELPLLARLDMDIRDRVATPASWYEPFREASWEEAMELAAQELGRIKHER
                     GPDALACFSSAKCSNEENYLLMRLFRGAIGTNNVDHCTRLCHSTSVAAMQRALNTSAA
                     SGSMREVEHDSDVIFIAGANTTETHPVFGAAIKRAHERGATLIVADPRRTELAGRADI
                     HLQMLPGTDVALFSAMLNHILAAGLENREFIAGRMREFESVRTCVQPYTPERAAKITG
                     VSAALIRRAAELYARGPRTSTLWAMGLTQHANGTDLVTALLNLMLACGMIGRWGAAMM
                     PVRGQNNVQGASDVGAIPFVYTDYQSVTDPAVRERFARVWGLSPEALSLQKGLMVTEI
                     VKPESGVRGMYIMGENPIISDPDIAHAEHWFQGLEFLAVQDLFLTETARYADVVLPAT
                     SFAEKTGTFVNTERRIQLSHRALDPPGDARGDLEILIELSRRLGLATPFQSAEDVMRE
                     IAAVTPSWAGVTYARLEGAGLQYPVPTTEHPGTAFLFDRAFPTADGKGIFVPVEYTDP
                     VELPDDQYPFVMNTGRQLYHWHTGTMTRRASGLDAREPTAIVEIHPDDATSLGVTEGD
                     LVRLTSRRNSMVTAVRVSDRVAPGQVFVPFHFREAAANLLTNPVLDPYAKMAELKICA
                     VRIEAASGELSGVGTARS"
     gene            5588..9085
                     /locus_tag="AC16JC_05905"
     CDS             5588..9085
                     /locus_tag="AC16JC_05905"
                     /inference="COORDINATES: protein motif:HMM:NF023400.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HIR5164265.1"
                     /translation="MRDTRLTLQGFRIAPPVPGSLWWTRYGQVEFRPDSVLQPSTEYQ
                     LIFDTTFRAPDGLPLLRSDTVAVRTLGPAVLATWPVTAYLPAMFVTSRTRFRFVMSEP
                     TDPEAWADSLHMAPQGYYRPSHPCRSLTAVRFRAQRPRPLHGNDSLFREIVKDSLLLV
                     RAPPTLLTVVPDRDLPAGCAGTLNNRPFLTAGEFRMRSTRSIPTEPVPAELRYVVGVE
                     FLSTTPVSESELMRHVRISPPMPVRFVEGRSPNGQPATAWEVEAGFAPATGYRLSADS
                     ALRDAFGQRLAEAFNFSFQTAPAKPGLRYRSTHRTVASHAVPSVDVTSINVSDLRVCT
                     ARVPDSARTQLVGGGWWRWSGIDSTTSESTECTRLAVRASPRDSLHTTVEVPWKAGWR
                     SRPAGLYAVRISSPQTPGDSRGPAMVVFHVTDLAVHARMDRDQATVLVAQRSSGAPVP
                     GAAVTAKTCSGDLIATGTTDASGVAELAGMSAREAHARFDCEMSTWRTLEVATPDDYD
                     ATNVPLSDDPHALVGALPASLIIPDRLVYRRGDTIRLRAIVRPPSPEYIRWVVVGSDR
                     DGLERRPVLDTLIPLSHAGTVELVFVVGPRLAPEIYEAALLVSRAGRWRQLADAEFWV
                     TGDPPPRVYLALTPARRWGARGDSVRFAVEAWNRDGTPASGVVAVTWTGGRLNPYAVV
                     TVPAGFTTSEVSVPPGEDSPEATGGARELTLDAAGKGGFTLRLPQRAPSWPWRVAVRA
                     TMRAGGPTQDGRAAVDVYPAAFNLAISAHSEEDGAVSDADDSVDVVALRPDGTPVSGV
                     RIDGLLAGRKWSPAPQEGDSLPSAGATVPDTLDQCTATTTNRPARCVLRRFATGYAWT
                     SFRATDSQGRVVEIGRLVSYPRDYGWNLERSLERPALAVNRTRFVVGDTAVVRIGSDA
                     PGGIAWLTVAFADRVESRVVRLSGGVDTVRLPIQARHVPRMILGATIWYPERDGASLT
                     HATKTSEIDLEIRDPTEAMTVAMQLPDRAEAASMRRISITVDSGSAAGDPPEILVWAL
                     DERLAAVDSQRLPDIVGTLLRPPLARRVTVSSLEDPAPVAFEPVDPGSSLPPSAFDMG
                     PDVLRYGPWGERSRQLDDLPGPQLLGMTRAGPDGTATLDARLPEAAGRYLVIVVAVTS
                     SRLGSTQGLLTLDRLPYRR"
     gene            9121..9912
                     /locus_tag="AC16JC_05910"
     CDS             9121..9912
                     /locus_tag="AC16JC_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5164266.1"
                     /translation="MNLGISGRVALVCGSTRGIGRAVAHALAVEGVRVALNGRNETEV
                     QQEASNLARETGQMVLAAAGDVAVPEQAKGLVERIHHELGRLDVLFCNAAGPPPARFV
                     DQPPEAWQRALDLNLLSTVHLCRAAVPLMRKSQWGRIVCLTSIAAKQPLPGLILSSTA
                     RAGVLGFAKALADEVAHFGITVNVVCPGYIATERTKELAASRAEIERRSLEEVTKQTT
                     EAIPLGRMGRAEEVASAVAFLASEPASYITGTVLQVDGGFTRSIL"
     gene            9909..>10565
                     /locus_tag="AC16JC_05915"
     CDS             9909..>10565
                     /locus_tag="AC16JC_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR5164267.1"
                     /translation="MTSSADFPLDMSNVMQAIVKERRAPGLLVATVPKPRAGAGEVLI
                     AVRHAGVCGTDLHIADWDAWAQGRLNPPLVIGHEFAGEIVEVGTGVTELTRGQLVTAE
                     GHIICGHCLQCRTGNGHICKNTRIIGVDRDGAFAAFIAMPATNVLPLEGIPTEIGAIM
                     DPMGNAFHTVLTAEIPGSTVFIVGCGPIGCFAVGIARAAGAARVIASDVNPKRLSLAR
                     T"
BASE COUNT         1614 a         3745 c         3473 g         1733 t
ORIGIN      
        1 tcagagtagt gaattgccgt ttggtctaaa tccgtaagtc tttgtaaata aatatgttat
       61 gagcttacct cgcatggccc cgcgtttgcc ttacctcggt acggacgcat caccttcttc
      121 aggggccaca tgacaagtct gccactcatt ctcgacgacg agcctctcgg catcattctg
      181 cgcgccggcc agcggcggct tcgcccggcc aggatctggg cctactgctg gtgcgccgac
      241 gatacagatt cctgcgatca cgtccaccag ctgttgccgg tggatccgca ccccgcgggg
      301 cggtaactcc ggggtttctg agtcttctgg tcgacgcggg gccggcgaca tccgccggcc
      361 ccgcgtcgtt ttgtctggaa ggaggatcag gtcggcgaga cgtcgatggt ggtcgtcagg
      421 ttgaactccg gattcccgca taccgtggcc tgcaccgtat accggccggg ccgcacgacg
      481 acgtcggccg gaatggggat gagcacgcga aacaccggac cgacgcggat cacgtagggc
      541 ggccgctcct gcggcaccag ccacgcgttg cgccagcccg gatcgcgctc cccgggcgcg
      601 cgcaccgaca cgcagatgaa ctcgttgttc gagaactcga acgtcaggtt ggtctgtccc
      661 tggacggtga tcttgtagta gatcagcggc gtgatgccgc ccgtcgaatc gcccgggacc
      721 gcggtgaagc gatgcggcag gacggccagc ctcacgttgg agtgatgcga cgggctgacc
      781 acctcgccgc tcgatccgct gtcgggctcg acgtcacgga tctgtaccgg cgtgaaccgg
      841 atgccttgcg gcggctggct gcccagctca ccgcgggcct cgatataccg gctgtcccag
      901 ttgcgccacc tggccgcgtc gccgcccaac ggcaggatcc acatttgcgt gctgccgatc
      961 aggatgggtt ccgtcagcac catcaccgcg cgccccgcgc cgcccggcgt ctcgccgctg
     1021 accgccacga caccctggag ggtcagagtg gtgtcggaag tcgggctgac gccgagcagc
     1081 gcggtcccgc agcacacgat cgtcagaaac atgagcgcct cccgtggccg gtcgccccac
     1141 ccacccgccg cccaccctag tctatcgcag agctgcggcg gtggtcagcc tggcgcgacg
     1201 cacgcgcttg ccccacccag cgggaatccg tagtctacag aatcatgatg tcgatcacga
     1261 tcaacggacg cccgctctcc gccgcccccg aacagaccat tctcgatgcc gcccgggcgg
     1321 ccgggatcga catccccacg ctgtgctggt atcccaccct gccgatcgtc ggcaactgcc
     1381 gcatctgtct cgtctcggtg gagggccagc ccaagctgct ccccgcctgc gccaccaagg
     1441 tggctgacgg catggtcgtg accaccgaat cacacgccgc cgtggagaac cgcaaggcgg
     1501 tgctgcactt cctgctggag cgctacccgg gggagcatct gcgaaacggc gaccgggcgg
     1561 acccgcgcaa cgagttcgag cagtacgtgg tccggtacgg cgtcacgcct tcccccgccg
     1621 ccggtcgcga tctgccgctc cgctcgggcg acgagcggcc cggcgatccg atgatccggc
     1681 acgacatgac gacctgcatc ctgtgcaccc gttgcgtgcg ggcgtgcgag gacctccagg
     1741 tcgtcggggt gctcgacgtc gcgcagcgag gcgagcacgc cgagatcatc gtcggtgcgg
     1801 acggcgatcc ggaccgggcg gggtgcacct ggtgcggcga gtgcgtccgg gtgtgcccca
     1861 ccggggcgat cttcgaggtc atgccccggg agcggttccg tcccgagcag gtgcgccggc
     1921 ccgaaaccat cgtgcgcgcc gtctgcccct attgcggcgt cggctgccag gtggatctgc
     1981 acaccgccga cgacaagatc gtccgcgtca cttcccccga catcgagctc gacacgccga
     2041 accagggcag cacctgcgtg aagggccgct tcggctacga tttcccgcag catcgcgacc
     2101 ggctcacccg gccgctgatc cgccggggct ggcagcgtga ggcggacggc cgctgggtgt
     2161 atcgcggggc cgggggggcc gaccggcgcg ccgggccgtg gcgcaccatc gaaggcgagg
     2221 gccagcagtc caagcccggc acgcccccgc gcaagacggg gaagtcggtg cgcgagctgc
     2281 cgctgctggc acggctggac atggacatcc gcgaccgggt ggcgactccg gcctcctggt
     2341 acgagccgtt ccgcgaggcc agctgggaag aggcgatgga gctcgccgcg caggagctgg
     2401 gacgcatcaa gcacgagcgc ggccccgatg ccctggcctg cttctcctcg gccaagtgct
     2461 cgaacgagga gaattacctg ctgatgcggc tgttccgcgg tgcgatcggc acgaacaacg
     2521 tcgatcactg cacccggctg tgccactcca cgtcggtggc ggcgatgcag cgggcgctca
     2581 acacctcggc ggcctcggga tcgatgcgcg aggtggagca cgacagcgac gtgatcttca
     2641 tcgccggcgc gaacacgacg gagacccacc ccgtcttcgg agccgccatc aagcgcgcgc
     2701 acgagcgggg cgccacgttg atcgtcgcag acccgaggcg caccgagctc gccggtcgcg
     2761 ccgacatcca ccttcagatg ctcccgggga ccgacgtcgc cctgttctcg gcgatgttga
     2821 atcacatcct cgcggcggga ctcgagaatc gggagttcat cgccggccgg atgcgggaat
     2881 tcgagagcgt gcgcacctgc gtccagccct acacccccga gcgcgccgcc aagatcaccg
     2941 gcgtctccgc cgccctgatc cggcgcgccg ccgagctgta cgcccgcggg ccgcgcacca
     3001 gcaccctctg ggcgatgggc ctgacgcaac acgccaacgg cacagacctc gtcaccgcgc
     3061 tgctcaacct gatgctcgcc tgcggcatga tcggccgttg gggggcagcg atgatgccgg
     3121 tgcgcggcca gaacaacgtg cagggcgcgt ccgatgtcgg tgcgataccc ttcgtgtaca
     3181 ccgactatca gtccgtgacc gacccggccg tccgcgagcg gttcgcccgc gtctggggcc
     3241 tctcccccga ggcgctctcc ctccagaagg gcctcatggt cacggagatc gtgaagccgg
     3301 agagcggcgt ccgcggcatg tacatcatgg gggagaaccc gatcatctct gatcccgaca
     3361 tcgcccacgc ggagcactgg ttccaggggc tggagttcct ggccgtgcag gacctgttcc
     3421 tgaccgagac cgcccgctac gccgatgtcg tgcttcccgc cacgagtttc gctgagaaga
     3481 ccggcacgtt cgtgaatacg gagcgccgca tccagttgag ccaccgtgcc ctcgatccgc
     3541 ccggcgacgc ccgcggcgac ctcgagatcc tgatcgaact gtcgcgccgg ctcgggctgg
     3601 cgacgccgtt ccagtccgcg gaagacgtaa tgcgggagat cgcggccgtc accccatcgt
     3661 gggccggcgt gacctacgcc cggctcgagg gcgcgggtct gcagtacccg gtgccgacga
     3721 ccgagcatcc gggcacggcc ttcctcttcg accgcgcgtt ccccaccgcg gacggcaagg
     3781 ggatcttcgt ccccgtcgag tacaccgacc cggtggagct ccccgacgac cagtatccgt
     3841 tcgtcatgaa cacggggcgg caactgtacc actggcatac gggaacgatg acgcgccgcg
     3901 cctccgggtt ggacgcgcgc gagccgaccg cgatcgtcga gatccatccc gacgacgcca
     3961 cgtccctggg cgtcaccgag ggcgacctgg tgcgcctcac ctcgcgccgc aactcgatgg
     4021 tcacggcggt ccgcgtgtcg gaccgcgtcg caccggggca ggtcttcgtc cccttccact
     4081 tccgcgaggc cgccgcgaac ctgctgacca acccggtgct ggatccctat gcgaagatgg
     4141 ccgagctgaa gatctgcgcc gtgcgcatcg aggcggcgag cggtgagctg tcgggggtgg
     4201 gaacggcccg aagctgattg attggagtcc tcccttgaag ggctttagca cctggatgtt
     4261 gttggcgctt ctgacctcca ccgcgtcggg gcaggaaaag ccgcttcacg tggtggacat
     4321 ccggattccg tggggaccac cgcaggtcct tcgagtcgta ttcgatcaac cagtcgcgcc
     4381 cgacaggtgg tctcccggca gggtccctga gggcatccac atcgcgccgt cggtggccgg
     4441 acgctacgag tggccggatt cggtcacgtt gcgcttcacg ccgtccaagc cgttcgtgcc
     4501 gggcgtttat gccgtcaccc tggattcgac gttctctgga cgtgacgggg cacggctgga
     4561 acgctcactc acctatcgcc tgcagcctga ggcactgtgg acgagcgttg ccttacaacg
     4621 cccaggcacg tctccaaccg ctcccgtcga gctcgccttc aaccgggccg ccggcatcag
     4681 gggcacgtcg agcgaacgtc ctccgccggg attcaccctc accccagcag cggatggtcg
     4741 atggctgtgg gtcgattcgc ttcggcttcg gttcactccg gcccggccgt ttgtgaccgg
     4801 ggtgcggtat gcgatcgtca tcgataccgc gttccggggt ccctccggcg ccagggtgcc
     4861 cagtaacgtc gaattccgat tccccgatcc actctgggag acttggcacc accttcctga
     4921 gtcgcccaac gagccgttga tcgtcaagtt cagcgcggcg atcgcacccg gcgactctgc
     4981 gcaaggcctt ctgttgatcg acccgccagt cgacggagtc gttacgattc ccgactcggt
     5041 aacgctcgag ttcacgcccc gcacgggatt caggcccgcg gtgggctacg tcgcacgggt
     5101 ctcggaagga ctccaggccg cgagtggcgc acggctggca ggccctcgtg gcgctcagcc
     5161 gtcaggcccg gtattgattg gcgagttcgc cttctggcta cggcttctga catacccctc
     5221 cgatcttgta cggcccacgg agccgctcac cttccgcttc aaccaccggg tccaaccacc
     5281 atccgagggc ggaacgcagt tgccacgctg gtttcgcctc agtccgcccg tgcccggtca
     5341 cgtggagtgg cccgactcac agacccttcg cttcgtcccc gactccgcct tgatccctgg
     5401 aaggaagtac ctcgtcacgg tgggaaggga cctcgtgact cccgacggct ttcgtctgcc
     5461 cgactcgatc accgtgacag tcaggtcggg gatgcggctt ggaggcaccc gctactcggt
     5521 gtcccgatcg ggcccgattt cgataggagt atggcctggt ccgggcgtcg gccgcggggt
     5581 cgcactcatg cgtgacacgc ggttaacgct gcagggcttc cgtatcgctc cccccgttcc
     5641 agggtcgctg tggtggaccc gctatggcca ggtggaattc cggccggact ccgttctgca
     5701 gccgagcaca gagtaccaac tcatcttcga cacgacgttt cgggcgcccg atggcctgcc
     5761 gctgctcagg tccgacacag tcgcggttcg cacactcggg cccgcggtcc tcgccacgtg
     5821 gccggttaca gcgtacctcc ctgccatgtt cgtcacctcg aggacgcgct tccggttcgt
     5881 catgtcggag ccgaccgacc ccgaggcgtg ggccgattcc ctccatatgg ccccgcaggg
     5941 ctattatcgc cccagccatc cgtgccggag tctcaccgcc gtgcgatttc gggcccagcg
     6001 cccgcgtccg ttgcacggca acgactcgct cttcagggag attgtaaagg actccctgtt
     6061 gctggtacgc gcccccccca ccctcctcac ggtggtcccc gaccgtgatt tgcccgcggg
     6121 atgcgcaggt acgctgaaca acaggccgtt cctcaccgcg ggcgaattcc gcatgcgttc
     6181 cacccgctcc atcccgaccg aaccggtgcc ggccgaactc cgctatgtcg tcggtgtgga
     6241 gttcctctct accactcctg tgagtgagag cgagctgatg cgtcacgtcc gcatctcgcc
     6301 accgatgccg gttcgcttcg tcgagggacg gtcgccgaac gggcagcccg ccacggcctg
     6361 ggaggtcgag gccgggttcg cgcctgccac gggctaccgc ctgtccgcgg acagtgcgct
     6421 ccgagacgcc ttcggccagc ggctcgccga ggcattcaat ttctcgttcc agacagcgcc
     6481 ggcgaagcct gggctgagat accgcagcac tcaccgaacg gtcgcgtcgc acgccgtccc
     6541 gagcgtggac gtcacatcga tcaacgtgag cgatctccgc gtctgtaccg cacgcgtacc
     6601 ggactcggca cgtacgcaac tggtcggcgg cgggtggtgg aggtggagcg gaatcgactc
     6661 gaccacgagc gaatcgaccg aatgcactcg cctggccgtg cgtgcctcac cccgggacag
     6721 cctccatacc accgtcgagg ttccctggaa ggcgggatgg aggtcgcggc ccgccggact
     6781 gtacgccgtg cggatctcga gtccccagac gccgggcgat tcgcgcggcc cggcaatggt
     6841 ggtgtttcac gtcaccgatc tcgccgttca cgcgcgcatg gatcgcgacc aggcgactgt
     6901 tctggtcgcg cagcgctcga gcggcgcgcc ggtgcccgga gcggccgtca cggcgaagac
     6961 ctgctccggc gatctcatcg ccacgggaac gaccgacgcc tcgggcgtgg ccgagttggc
     7021 cggtatgagc gcgcgcgaag cacacgctcg cttcgattgt gagatgtcga cctggcgcac
     7081 cctggaggtg gcaacgccgg acgactatga cgcgacgaac gttccgttga gcgacgaccc
     7141 gcacgccctg gtcggtgcgc tcccggcctc actcatcatc ccggaccgcc tggtgtatcg
     7201 gcgcggggat acgatacgac tgcgagcgat cgtgcggccg ccgtcccctg aatacatccg
     7261 gtgggtcgtc gtcggcagcg accgcgacgg actggagcgg cgtccggtgc tcgataccct
     7321 catcccgctc tcgcacgccg ggacggtaga gctggtgttc gtggttggcc cgcgcctcgc
     7381 acccgagatc tatgaggcgg cactcctggt gagccgggca ggacggtggc gccagttggc
     7441 cgatgccgag ttctgggtca ccggcgaccc gcctcctcgg gtctacctcg ccctcactcc
     7501 tgcgcggcgc tggggcgcgc gcggcgactc ggtgcgcttt gccgtcgagg catggaatcg
     7561 cgacggcacg cccgcctccg gagtggtcgc ggtgacctgg accgggggcc gcctgaaccc
     7621 ctacgcggtc gtcacggtgc cagcgggctt taccacgtcc gaggtcagtg tgccgccagg
     7681 agaggattcg cccgaggcga cgggtggggc tcgcgagctg accctggacg cagccggcaa
     7741 gggaggtttc accttacggc tgccgcagcg cgcgccgtcg tggccctgga gagtggcggt
     7801 gcgggcgacc atgcgggcgg gcgggccgac acaggatggg cgcgccgcgg tggacgtcta
     7861 tccggccgct ttcaacctcg cgatcagcgc tcattccgag gaggacggcg ccgtcagcga
     7921 cgcagacgac tcagtggatg tcgtggcact gcgaccggac gggacaccgg tgtccggcgt
     7981 caggatcgac ggtctcctgg cgggacgcaa atggagcccg gctccccagg agggcgactc
     8041 tctgccctcc gccggcgcca cggtgccgga cacactcgac cagtgcaccg cgacgacgac
     8101 gaatcgtccg gcgcgctgcg tgttgcgccg gttcgccacc gggtatgcct ggacttcgtt
     8161 cagggcgacg gattcgcagg gacgggtcgt cgagatcgga cggctggtct cctaccctcg
     8221 cgactacggc tggaatctcg agcgtagcct cgaacggccc gccctcgccg taaaccgtac
     8281 ccgcttcgtc gtgggcgaca ccgccgtcgt caggatcgga agcgacgcgc ccggcgggat
     8341 cgcctggttg accgttgcgt tcgctgatcg cgtcgagagc cgtgtcgtgc ggctctccgg
     8401 aggcgtcgac accgtgcggt tgccgataca ggcgcgccac gtcccccgga tgatcctggg
     8461 ggcgactatc tggtaccctg aacgcgacgg cgcctccctc acccatgcga cgaagaccag
     8521 cgagatcgac ctggagatcc gtgatcccac cgaggcgatg acggtggcga tgcagctccc
     8581 cgatcgcgcc gaggccgcct cgatgcgcag gatctcgatt acagtggaca gcggttccgc
     8641 cgcaggagat ccaccggaga ttctggtgtg ggccctcgac gagcgcctcg cggccgtcga
     8701 cagccagcgc cttcccgata ttgtcggaac gctcctgcga ccgccgcttg cccggcgggt
     8761 gacggtttcc tcgctcgagg atcccgcccc ggtcgccttc gagcctgtag acccgggttc
     8821 gagcctaccc ccatctgcat ttgacatggg ccccgacgtc ttgagatacg gcccgtgggg
     8881 cgagcgcagc cggcaactgg acgatcttcc cggcccgcag ctgctgggca tgacgcgtgc
     8941 tggtcccgac ggcaccgcga cgctcgacgc gcggctgccc gaagcggcgg gacgctacct
     9001 ggtgatcgtc gtcgccgtga cgagctcgcg gctcggaagc acacagggcc tgctcacgct
     9061 ggaccgactc ccttaccgcc gatagctgac cacttccaca ccgcctagat tctctcctgc
     9121 atgaacctcg gcatctccgg tcgcgtcgcg ctcgtctgcg gcagcacccg cggcatcggg
     9181 cgcgccgtcg cgcacgccct ggccgttgag ggagtccgag tcgcgctcaa cggacgcaac
     9241 gaaacggaag ttcagcagga ggcgtcgaat ctcgcccgcg aaaccggcca gatggtactg
     9301 gccgccgcgg gcgatgtggc cgtccccgag caggccaagg gcctggtcga acgcatccac
     9361 cacgagcttg gacgtctcga cgttctgttc tgcaacgcgg ccggcccgcc ccccgcccgc
     9421 ttcgtagatc agccgcccga ggcgtggcag cgcgccctcg atctgaatct cctgtccacc
     9481 gtgcacctgt gccgggccgc tgtgccgctg atgcgcaagt cgcagtgggg ccggatcgtg
     9541 tgcctcacct cgatcgcggc gaaacagccg cttccgggcc tcatcctctc gagtaccgcc
     9601 cgggccggcg tgctcgggtt cgccaaagcg ctggccgacg aagtcgccca cttcgggatc
     9661 accgtgaacg tcgtctgccc gggctacatc gccacggagc gcaccaagga gctggccgca
     9721 tcccgcgcgg agatcgagcg gcgcagcctg gaggaggtga ccaagcagac taccgaggcg
     9781 attcccctgg gccgcatggg ccgggccgaa gaggtcgcga gcgcggtcgc gttcctcgcc
     9841 tccgaaccgg caagctacat taccggcacc gtgctccagg tggacggcgg gttcacgcgc
     9901 tcgatacttt gacctcctct gccgacttcc ccctcgacat gtccaacgtg atgcaggcga
     9961 tcgtcaagga gcgacgcgcc cccggcctcc tggtcgccac cgtgcccaag cctcgcgccg
    10021 gcgccggcga agtgctcatt gccgtgcggc acgccggcgt gtgcgggacc gacctgcaca
    10081 tcgccgattg ggacgcctgg gcccagggtc gcctcaaccc gccgctcgta atcggccatg
    10141 aattcgccgg cgagatcgtg gaggtgggaa ccggcgtcac cgaactgacg cgcggtcagc
    10201 tcgtgaccgc cgaggggcac atcatctgcg gccattgcct ccagtgccgc accggcaacg
    10261 gccacatctg caagaacacg cgcatcatcg gggtggaccg cgacggcgcg ttcgccgcat
    10321 tcatcgcgat gcccgcgacc aacgtcctgc cgctggaggg gatcccgacc gaaatcggcg
    10381 ccatcatgga cccgatgggc aacgcgttcc acacggtgtt gacggccgag atcccgggca
    10441 gcacggtgtt catcgtggga tgcggtccca tcggctgttt cgccgtgggc atcgcccggg
    10501 cggccggcgc cgcgcgcgtg atcgcctcgg acgtcaatcc caaacggctc tcgctggccc
    10561 gcacc
//
LOCUS       DBNGZW010000248         2441 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1787753, whole genome shotgun sequence.
ACCESSION   DBNGZW010000248 DBNGZW010000000
VERSION     DBNGZW010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1787753"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..902)
                     /locus_tag="AC16JC_05920"
     CDS             complement(<1..902)
                     /locus_tag="AC16JC_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015877525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR5164268.1"
                     /translation="MPHDVSLITTIAAGLGLALVMGFLATRVGVPPLVGYLVAGIILG
                     PVTPGFVADVELTRQLAELGVMLMMFGVGLHFSLDDLLAVRRIAIPGAVVQSVVATAL
                     GAATALAWGWSPGAGIVFGLSLSVASTVVLLRALEGQRLLRSVNGQIAVGWLVVQDLV
                     VVVVLVLLPALAGLLGGTAPAVTAGAQSTGTTLALTLGKVAAFVALMLVVGRRLFPKL
                     LWMVARIGSRELFTLAVISVAVGVAYASSRLFNVSFALGAFFAGMMMRESALSHRAAE
                     ESLPLRDAFAVLFFVSVGMLFDPS"
     gene            complement(906..1160)
                     /locus_tag="AC16JC_05925"
     CDS             complement(906..1160)
                     /locus_tag="AC16JC_05925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164269.1"
                     /translation="MSETIDRDAVTDARPPRWSRTRTIWALLALSLAPGVVAGAAREW
                     WHQLPAGARLSAYILSGILLVAACSLMLMQSEAKSGNTET"
     gene            complement(1309..1671)
                     /locus_tag="AC16JC_05930"
     CDS             complement(1309..1671)
                     /locus_tag="AC16JC_05930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164270.1"
                     /translation="MHRTRASWSALLLALLLGILPRLGAPVDARAGAATLGPAAGPPS
                     PDAYHLRAAASFRQALEWRSPTDRQLTTSGGALPTRPMLPILQGVQPGVLASHPAARH
                     AGAGCRFPYFPTGPPSRT"
     gene            1753..2190
                     /locus_tag="AC16JC_05935"
     CDS             1753..2190
                     /locus_tag="AC16JC_05935"
                     /inference="COORDINATES: protein motif:HMM:NF039479.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6524 family protein"
                     /protein_id="HIR5164271.1"
                     /translation="MAKAGIGPAGLLLRILGATLLVYATFNPTGHSFYHWALAPLVGL
                     GSASGGSAALKFLVGILLLIGWVVVLQATRRSIGLTGAGLALALCGGLIWLLVEQHVV
                     SPTGSAAIAHIVLLAVGLVLGVGMSWSLLSRRLTGQVDTDQVG"
     gene            complement(2178..>2441)
                     /locus_tag="AC16JC_05940"
     CDS             complement(2178..>2441)
                     /locus_tag="AC16JC_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5164272.1"
                     /translation="FDLFMLDTRLRDASALAALRAVGEHAPNTPILSHAAFLTVETRH
                     AARQRGAWDVVVRGGLNSMWSAMSNLLAVSASELHARPGLQPT"
BASE COUNT          401 a          842 c          890 g          308 t
ORIGIN      
        1 atggacgggt cgaacagcat gcccaccgag acgaagaaga gaaccgcgaa cgcgtcgcgg
       61 agcggaagcg actcctcggc cgcgcggtgg ctcagcgccg actcgcgcat catcatcccg
      121 gcgaagaagg cgcccagcgc gaaggagacg ttgaacagcc tggaggaggc gtaagccacg
      181 cccaccgcca cggagatcac cgccagggtg aacagctccc gcgagccgat gcgcgccacc
      241 atccagagca gcttcgggaa taatcggcgt cccacgacga gcatgagcgc cacgaatgct
      301 gcgaccttgc ccagcgtcag cgcgagcgtg gtgccggtgc tctgcgcccc cgccgtcacc
      361 gcgggcgccg tgccgccgag cagcccggcc aaagcgggca gcagcaccag caccaccacc
      421 acgaccagat cctgaaccac cagccagccc acggcgatct gcccattgac cgagcgcagg
      481 agccgttgcc cctcgagtgc gcgcagcagg acgacggtgc tcgcgacgga cagcgagaga
      541 ccgaagacga tgccggcccc gggactccag ccccacgcca gcgcggtagc cgcgcccagc
      601 gccgtggcga ccacgctctg caccacggcc ccgggaatgg cgatgcggcg caccgccagc
      661 aggtcgtcca gcgaaaaatg cagcccgacc ccgaacatca tcagcatgac gccaagctcg
      721 gccagctggc gggtcagctc gacgtcggcc acgaacccgg gggtgacagg gccgaggatg
      781 atcccggcca cgaggtagcc caccaggggc gggacgccga ctcgcgtggc caggaatccc
      841 atgaccaacg ccagccccag gcccgcagct atcgtggtga tgaggctgac gtcgtgtggc
      901 atgcgctatg tctccgtatt gccggacttc gcctcgctct gcatgagcat gagcgagcag
      961 gctgccacga gcaggatgcc cgacagaatg tacgccgaca gccgggcgcc tgcggggagc
     1021 tggtgccacc actcccgggc cgccccggcg acgaccccag gggcgaggct gagggcgaga
     1081 agggcccaga tggttcgcgt ccgcgaccag cgcggcggac gcgcgtccgt gacggcgtcg
     1141 cggtcgatcg tctccgacat gcgagcttct ccttgcgcgg ccgcgtccgc ggccgggggg
     1201 cgcagcgatg cgtcccgggc ctgcggcgcg tgagggccgc gcggcagttc gagctatggg
     1261 gagtaggaga gcgggccgac aacccggccg gcgccgctca ggcctgcatc aggtgcgcga
     1321 aggagggccg gtcgggaagt acgggaagcg gcatcccgct ccagcgtgac gcgcagcggg
     1381 gtgcgaggcg aggaccccag gctgcacccc ctgaaggatc gggagcatgg ggcgggtcgg
     1441 aagcgcgccg ccggaggtcg tgagctgtcg gtcggtcggc gagcgccact cgagcgcctg
     1501 gcggaagctg gcggcggctc ggaggtggta cgcatcgggc gacgggggtc cggccgcagg
     1561 tccgagtgtc gcagcgccgg ccctggcgtc cacgggcgcg cccaagcgcg gcaagatgcc
     1621 gagcaggagt gcgagcagca gagccgacca ggaggcgcgc gtgcgatgca tgccgccaac
     1681 ctaggcaggt aggcggagga ttggtaccct tgtgagacct ttcgatccga acctcacacc
     1741 acggagcgtg gcatggccaa ggcgggcatc ggtccggccg gacttctcct gcggatcctg
     1801 ggcgcgaccc tcctcgtgta tgccaccttc aacccgaccg gccactcctt ctaccactgg
     1861 gcgctggccc cgctggtggg tctgggctcg gcgtccggcg gctcggcggc gctcaagttt
     1921 ctcgtaggga tcctgctgct gatcggctgg gtggtggtcc tgcaggcgac gcgccgctcg
     1981 atcggcctca ccggtgcggg gttggcgctt gcgctgtgcg gcgggttgat ctggctcctg
     2041 gtcgagcagc acgtggtctc acccacgggc tcggcggcga tcgcgcacat cgtgctgctc
     2101 gccgtcgggc tcgtgctcgg cgtggggatg agctggtcgc tcctctcccg gcgcctaacc
     2161 ggtcaggtcg ataccgatca ggtaggctga agccccggcc gggcgtgcag ctccgacgcc
     2221 gagaccgcca gcaggttgct catcgccgac cacatggaat tgagcccgcc acggacgacc
     2281 acgtcccaag cgccccgctg gcgagcggcg tgccgcgtct cgaccgtcag gaaggcggcg
     2341 tgggacagga tcggcgtgtt gggcgcgtgc tcgccgacgg cccgcagcgc ggccagcgct
     2401 gaagcgtcgc gcagccgggt gtcgagcatg aagaggtcga a
//
LOCUS       DBNGZW010000249         4905 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1453867, whole genome shotgun sequence.
ACCESSION   DBNGZW010000249 DBNGZW010000000
VERSION     DBNGZW010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4905
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1453867"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="AC16JC_05945"
     CDS             complement(<1..914)
                     /locus_tag="AC16JC_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164273.1"
                     /translation="MRALLAADFERFRDGLPRDFPAYVREAYKVDLTARYLGQTLPHP
                     IGKGSGQLSLNVRQLEEDSAAGLAFVVLKTLIAQDAAGVRSMAAWAVHETKMKVERRM
                     VAGADRGWTVTWRGRGWDRSLEDYLALVRVGRDLTRAGALLVFPSVKYHLPRLGEPFV
                     ESEYRFTTAALADAWGEEPLLVEKDFSPTLAGDALAEDRAQILRWLRETPQLIRGVSP
                     IETRVSVKLMNARFEDDFQHDMMLAAAGADALVCFNRLFDVERGVAYGGTDLLERNLR
                     ILRSFSRLGGDLERSPFPALCGTGGIGSG"
     gene            complement(892..1770)
                     /locus_tag="AC16JC_05950"
     CDS             complement(892..1770)
                     /locus_tag="AC16JC_05950"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0071704 - obsolete organic substance
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HIR5164274.1"
                     /translation="MSLIARLEGGLIPAVPVPFQGSGVAAAAQQRYAVWMAGQAVAGV
                     AVWAHTGRGPHLTEAQRRSVLDVWRAALPDRVVVAGASTPEMAREARAGGADALLAFP
                     RATDPVGWHAALSAELPVIAFYLYGAAGGVAYDEGTLAAILALPGVVGVKVATLDSVM
                     TFQRIAALLRAAPGKLLITGEDRFLGYSILLGASAALVGMGAALTDLQCDLLRAHVAG
                     DAARFNRLLGILDRFAQATFVPPMEGYVRRMLWAAAADGVIPADACDDPWAPAEVPGE
                     RAAVIRAVHDARAAGR"
     gene            complement(1774..2547)
                     /locus_tag="AC16JC_05955"
     CDS             complement(1774..2547)
                     /locus_tag="AC16JC_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5164275.1"
                     /translation="MRIDVNAFLGAYPWSRLPGTSPEALLAAMDRVQIDTAWVTHLPG
                     VFWRDPTEGNPWLYETCRAYPRLRPVAAVHPGLAHWQDVLNEAVERGAAAVRCDPLYF
                     GLPPSGPEVRVLAAACGAANMPLLMAVRLEDGRQRHPNDVAGELPAAALRALIRCDQD
                     LRLVVTHADRGTVEEVHFGSTPEEARRISWDISWLWGPPEEHLAAVLESVGVERFVFG
                     TGQPLRLPENVAAKLDLLDLSAEQRASIESDNARALSAG"
     gene            complement(2547..3347)
                     /locus_tag="AC16JC_05960"
     CDS             complement(2547..3347)
                     /locus_tag="AC16JC_05960"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5164276.1"
                     /translation="MLIDVHAHFFPERSLRADWRAYNASRLRAGERIGIGIHVTSTLG
                     TFGRTSPTYFPSPADLVYGNDFSLALQREHAARVRCYACVNPNYTGHALAEIARCRTA
                     GMVGIKLAASRRASDPLLDPICHLAAELKLPILHHIWQGRRRDWPGQEASDGVELCQL
                     AARHQDVTFILAHVGGGGDWRHTLPAVRPHRNVYLDLSGSGVDGGMLEACLGAVGAAR
                     LLWGTDMTMETGWGKLRYLERQLSPADLELVRWKNATRIFPSGSFPTD"
     gene            complement(3379..4542)
                     /locus_tag="AC16JC_05965"
     CDS             complement(3379..4542)
                     /locus_tag="AC16JC_05965"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5164277.1"
                     /translation="MNDWRVPGFTSTGSKRPEALFGTGDDPGLPARLARSAGARVWDA
                     EGREYLDYVMALGAVALGYGHPDVNRAAADAIAAGVVGPLPPVLEEVLAEALAGRLPW
                     LERIRFLKTGAEAVAAAVRIARVVTGREEILGCGYHGWLDWCQGRAEGVPQGTRAVFA
                     ELPFNDPERAREMIRYRSDRLAAVVIEPVVVSEPSREWLEVVRAETTEVGAVLVYDEI
                     KTAFRLAIGGAAERYGIVPDLMVIGKACANGFPLAAVGGRADLMAAVNRTWISSTLAT
                     ESVALAAALATLEVFEREQVCAHLHQVGTELLHGLHRLQHHHRHVLTGVGGVAEMCFF
                     HYVNEDVSRAVAAGCARRGLLFKRTAYNFVSLAHGAADIAFTLGVLEEVLKSA"
     gene            complement(4499..>4905)
                     /locus_tag="AC16JC_05970"
     CDS             complement(4499..>4905)
                     /locus_tag="AC16JC_05970"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR5164278.1"
                     /translation="GLSNEALDRCHRIVTIPTSPAYASLNLAHAVVVMLYELALARGD
                     EERAFKTPRRPAEAATVEEIERLFGDAERALAAIDFFKIRDREHIMRTVREIVHRVPL
                     DQREAKLLRAMAIEVVKYRERLARTGIHVDRE"
BASE COUNT          780 a         1782 c         1691 g          652 t
ORIGIN      
        1 ccggagccga tgccccccgt gccgcacagt gcgggaaacg gggaccgctc gagatcgccc
       61 ccgagccggg agaagctgcg aaggatgcgc agattgcgct cgagcagatc ggtgccgccg
      121 taggccacgc cgcgctcgac gtcgaacagc cggttgaagc acaccagggc gtcggcgccg
      181 gcggccgcca gcatcatgtc gtgctgaaag tcgtcctcga agcgcgcgtt catcaacttc
      241 accgacacgc gggtctcgat gggggaaacg ccgcggatga gctggggcgt ctcccgcagc
      301 caccgcagga tctgcgcccg gtcttcggcg agcgcgtcgc ccgccagcgt cggcgaaaag
      361 tccttctcca cgagcagcgg ctcttcgccc caggcgtcgg ccagcgcggc ggtggtgaag
      421 cgatactccg attcgacgaa cggctccccg aggcgcggca ggtggtactt caccgagggg
      481 aagacgagca gtgccccggc tcgcgtgaga tcgcgtccca cccgcaccag cgccagatag
      541 tcctcgaggg agcggtccca gccacggccc ctccaggtga cggtccagcc gcgatcggcg
      601 ccggccacca tgcgacgctc gaccttcatc ttcgtctcgt gcacggccca ggccgccatc
      661 gagcggacgc ccgccgcgtc ctgcgcaatc agggtcttga gcaccacgaa cgccagtccg
      721 gccgccgagt cttcctcgag ctgccgcaca ttgagcgaca gctgccccga tcccttgccg
      781 atcggatggg gcaacgtctg ccccaggtag cgggcagtga gatcgacctt gtacgcctcc
      841 cgcacgtagg ccgggaagtc ccgcggcagg ccgtcgcgga agcgctcgaa gtcagcggcc
      901 agcagcgcgc gcatcgtgca ctgcccggat cacggccgca cgctccccgg gcacctcggc
      961 cggcgcccac gggtcgtcgc aggcatccgc gggaatgacg ccgtcggcgg cagcggccca
     1021 gagcatgcgc cgcacgtacc cttccatcgg cggcacgaac gtagcctgcg cgaaccggtc
     1081 caggatgccg agcagccgat tgaatcgggc agcgtcgccc gcgacatgcg cgcgcaacag
     1141 atcgcattgc agatccgtca acgccgctcc catcccgacc agcgcggcgc tcgcccccag
     1201 caggatcgag tacccgagga accggtcctc ccccgtgatg agcagtttgc cgggagccgc
     1261 gcgcagcaac gcggcgatgc gctggaacgt catgacggaa tccagggtgg ccaccttgac
     1321 gccgacgacg cccgggagcg cgagaatcgc cgccagcgtc ccctcgtcat aggccacgcc
     1381 gcccgctgcc ccgtataaat agaaggcgat gacgggcagc tccgcgctca acgcggcgtg
     1441 ccagccgacg gggtcggtcg cgcgggggaa ggcgagcagt gcgtcggcgc cgccggcccg
     1501 ggcttcgcgg gccatttcgg gcgtcgacgc gcccgccacg accacgcgat cgggcagggc
     1561 ggcgcgccag acgtcgagca cgctgcgccg ctgggcctcg gtgagatgcg gaccccgtcc
     1621 ggtatgggcc cacacggcga cgcccgcaac ggcctgcccc gccatccaca cggcgtacct
     1681 ctgctgggcg gcggcggcga cgcccgagcc ctggaacgga accgggacgg cggggatgag
     1741 cccgccctcg aggcgcgcga tcagcgacac ggctcagccc gccgacaggg cgcgcgcgtt
     1801 gtccgactcg atcgacgcgc gctgctccgc cgacaggtcg aggaggtcca gcttggccgc
     1861 gacattctcc gggaggcgca gcggctgccc cgtgccgaac acgaaccgct cgacgcccac
     1921 cgactcgagc acggccgcca ggtgttcctc gggtggaccc cacagccagc tgatgtccca
     1981 cgagatgcgc cgcgcctcct ccggtgtcga gccgaaatgc acttcttcca cggtgccccg
     2041 atcggcgtgc gtgacgacga gccgcaagtc ctggtcgcag cgaatcaggg cgcgcagcgc
     2101 cgccgccggc agctctccgg ccacgtcgtt ggggtgacgc tgccgtccgt cctccagtcg
     2161 gaccgccatg aggagcggca tgttcgcagc accgcaagcc gccgccagca cgcggacctc
     2221 gggcccggat ggcggcaaac cgaagtagag gggatcgcac cgcacggccg cggcgccccg
     2281 ctccaccgcc tcgttgagca catcctgcca gtgagccaga cccggatgca ccgctgcaac
     2341 gggccgcagt cgcgggtagg cgcggcaggt ctcgtacagc caggggttcc cctcggtcgg
     2401 atcacgccag aacacaccgg gaaggtgagt gacccacgcc gtgtcgatct gcacgcggtc
     2461 catcgccgcg aggagcgctt cgggggaagt acctgggagg cggctccagg gataggcgcc
     2521 gaggaaagcg ttgacgtcga tacgcatcag tcggtgggga agctgccgga cgggaagatg
     2581 cgcgttgcgt tcttccaccg gacgagctcg aggtcggccg gagagagctg tcgctcgagg
     2641 tagcggagct ttccccatcc cgtctccata gtcatgtcgg tgccccacag gagccgcgcg
     2701 gcgcccaccg ccccgaggca agcctccagc atgccgccgt cgacgccact cccggagagg
     2761 tcgagataga cgttgcggtg cgggcgcacg gccgggaggg tgtggcgcca gtcgccgccg
     2821 ccgcccacgt gggccaggat gaaggtgacg tcctgatgcc gtgcggcgag ctggcacagc
     2881 tcgaccccgt ccgaggcctc ctgcccgggc caatcgcggc ggcggccctg ccagatgtgg
     2941 tgcaggatcg gcagcttcag ctcggccgcg aggtggcaga tgggatcgag cagcgggtcg
     3001 ctggcccggc ggctggccgc gagcttgatg cccaccatgc ccgccgtgcg gcagcgggcg
     3061 atttcggcga gcgcatgccc ggtgtagttc gggttgacgc aggcataaca gcgcacgcgt
     3121 gcggcatgct cgcgctggag cgcgagcgag aagtcgttgc cgtacacgag gtcggccggg
     3181 gacggaaagt acgtcggcga ggtgcggccg aaggtgccga gcgtcgacgt gacgtggatc
     3241 ccgatgccga tgcgctcccc cgcccgcagc cggctggcgt tgtaggcgcg ccaatcggcg
     3301 cggaggctcc gctcgggaaa gaagtgggcg tggacgtcga tcagcatgca gaaatctgat
     3361 ccgggcggca ggaggacgct acgccgactt caagacctcc tccaggactc cgagggtgaa
     3421 cgcaatgtcc gcggcgccgt gggcgaggga cacgaagttg taggcggtgc gcttgaacag
     3481 cagtccccgt ctggcgcagc ccgccgccac cgcccgggag acgtcctcgt tcacgtagtg
     3541 gaagaagcac atctcggcca ctcccccgac gccggtgagg acatggcggt ggtggtgctg
     3601 caagcgatgc agtccatgca gcagctcggt gccgacctgg tgcaggtgcg cgcagacctg
     3661 ctcgcgctcg aacacctcca gcgtcgccag cgcggcggcg agggcgaccg attcggtggc
     3721 gagggtggag gagatccagg tgcgattcac cgccgccatc aggtcggcgc ggccgccgac
     3781 ggccgccagg ggaaagccgt tggcgcaggc cttcccgatc accatcaggt cgggcacgat
     3841 gccgtaacgc tcggcggccc cgccgatcgc cagccggaac gccgtcttga tctcgtcata
     3901 gacgagtacg gcgcccactt ccgttgtctc ggcccgcacc acctccagcc actcgcggct
     3961 cggctccgac acgacgacgg gctcgatcac caccgccgcc agccgatcgc tgcggtaacg
     4021 gatcatctcg cgtgcccgct cggggtcgtt gaacggcagc tccgcgaaca cggcgcgcgt
     4081 cccctgcggc accccctcgg cccggccctg gcaccagtcg agccagccgt ggtacccgca
     4141 gccgagaatc tcctcgcggc cggtgacgac gcgggcgatc cgcaccgccg ccgcgaccgc
     4201 ttccgcgccg gtcttgagga accggatgcg ctccagccag ggcaaccggc ccgccagcgc
     4261 ctcggccagc acttcctcca agaccggggg caacggcccg accacgccgg ccgcgatcgc
     4321 gtcggccgcc gcgcgattca cgtccgggtg cccgtacccc agcgccaccg ccccgagggc
     4381 catgacgtag tcgaggtatt cgcgcccctc cgcatcccat actcgagcgc cggccgagcg
     4441 cgccagccgt gcgggcaggc cggggtcgtc gccggtgccg aacagcgcct cgggacgctt
     4501 actcccggtc gacgtgaatc ccggtacgcg ccagtcgttc acgatacttc accacctcga
     4561 tggccatcgc gcgcagcagt ttcgcctcgc gctggtcgag cggtacgcgg tgcacgatct
     4621 cgcgcaccgt ccgcatgatg tgctcgcggt cgcggatctt gaagaagtcg atcgcggcga
     4681 gcgcccgttc ggcatctccg aacagacgct cgatctcctc taccgtggcc gcctccgccg
     4741 gccggcgcgg cgtcttgaac gcccgttcct catccccgcg ggccagcgcc agctcgtaca
     4801 gcatcacgac cacggcgtgc gccaggttca gcgaggcata ggcgggcgac gtgggaatgg
     4861 tcacgatgcg atgacagcgg tccagcgcct cgttcgacag tccct
//
LOCUS       DBNGZW010000250         2301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_634266, whole genome shotgun sequence.
ACCESSION   DBNGZW010000250 DBNGZW010000000
VERSION     DBNGZW010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2301
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_634266"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..452)
                     /locus_tag="AC16JC_05975"
     CDS             complement(<1..452)
                     /locus_tag="AC16JC_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164279.1"
                     /translation="MKRLGVIGTMVWDTIHSRDPTQRAVEEWGGIAYALAGLDAALPD
                     DWAIVPLIKVGRDLAPRASAFLRDVRRVVPGSRFIEVPEPNNRVTLHYETAERRCEQL
                     TGGVPGWTWVELGGLVRDLDALYVKFISGWGLGLDTAQLLRRGFARPI"
     gene            complement(449..1417)
                     /locus_tag="AC16JC_05980"
     CDS             complement(449..1417)
                     /locus_tag="AC16JC_05980"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR5164280.1"
                     /translation="MRYIVGIDVGGTNIVAGTVAEDGSEILGVLSEPTKAEDGPDAVV
                     GRIVDLARASMADAGRGRDVAGVGIGSPGPLDTKTGVVLLTPNLGWVNMPLRDRVAGA
                     LGLPGTLDNDANCAVFGEWWRGAARGAEHVVGLTIGTGIGGGIVLYGDIYRGASDIAG
                     EIGHMTIDSTGRRCKCGNYGCLEAYASGPAIAARAVEGIEAGADSTLPRLVGGDLRKV
                     TAQVVYDAAHEGDEFALEVVRDTAKFLGAGVANIINIFNPQVVVICGGVTLAGDKLFV
                     PLQSEVKRRAFKPAVDVCRILPGELPGTAGVWGAAAVFIKRTWGMK"
     gene            complement(1395..>2301)
                     /gene="waaF"
                     /locus_tag="AC16JC_05985"
     CDS             complement(1395..>2301)
                     /gene="waaF"
                     /locus_tag="AC16JC_05985"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HIR5164281.1"
                     /translation="VAAPLLETHPAVRRVLAYDKRKADRGFAGLRRLARQLRAERYAV
                     AYLPHRSLRSAALARLAGIPRRVGYTDGWRSLYTEARPRPRDGHEIDRLLALVEATPH
                     QGALPTLHLVASDVARAEERLRAAAVAPPLAALAPGSIWGSKRWPHFAALARRLAQRC
                     DVVLVGGADDAALADAIVAAANDEDAAHRVVSLCGRLSLRESAAVIREAAILVTNDSA
                     PLHFAQAVGTPTVAIFGPTIPAFGFGPRGPRDVVVGLDGLACRPCSPHGPAACPLGHH
                     RCLQAVSVDDVLRAMEETGAIHRRD"
BASE COUNT          337 a          884 c          763 g          317 t
ORIGIN      
        1 tagatcggcc gggcgaagcc gcggcgcagc agctgcgccg tgtcgagccc cagtccccag
       61 ccggagatga atttcacgta cagcgcatcg agatcgcgta ccaggccgcc cagctcgacc
      121 cacgtccaac ccgggacgcc gccggtgagc tgctcacaac gccgctccgc cgtttcgtaa
      181 tgcagggtga cgcgattgtt gggctccggc acttcgatga accgggaccc cgggacgacg
      241 cgccgcacgt cgcgcaggaa ggccgatgcc cgcggcgcca ggtcccgtcc caccttgatc
      301 agcggcacga tggcccagtc gtcggggagc gccgcatcca gcccggccag ggcgtaggcg
      361 attccccccc actcctcgac cgcacgctgc gtggggtcgc ggctgtggat ggtgtcccac
      421 accatcgtcc cgatgacccc gaggcgcttc acttcatgcc ccacgtccgc ttgatgaaga
      481 ctgccgcggc gccccagacg ccggccgtgc ccggcagctc gcccggcagg atgcggcaca
      541 cgtcgacggc cggtttgaac gcccgccgct tcacctcgct ctggagcggc acgaacagct
      601 tgtcgccggc cagagtcacc ccgccgcaga tcacgaccac ctggggattg aagatgttga
      661 tgatgttggc cacgcccgca ccgagaaact tggcggtgtc ccgcacgacc tcgagcgcga
      721 actcgtcgcc ttcgtgcgct gcgtcgtaca ccacctgcgc cgtgaccttt cgcaggtccc
      781 ctccgactag gcggggcagg gtgctgtcgg cccccgcttc gatcccctcc accgcgcggg
      841 ccgcgatcgc cggtcccgac gcgtacgctt cgaggcaacc gtaattgccg cacttgcagc
      901 ggcgcccggt ggagtcgatg gtcatgtggc cgatttcacc cgcgatgtcc gaagcgccgc
      961 ggtagatgtc gccgtacagc acgatcccgc cgccgatgcc ggtgccgatg gtcagcccga
     1021 cgacgtgctc cgctccgcgc gcggccccgc gccaccactc gccgaacacg gcgcagttgg
     1081 cgtcattgtc caacgtcccc ggcaggccga gtgcgcccgc cacgcggtcg cgcaacggca
     1141 tgttgaccca gccgagattc ggggtgagca acacgacgcc ggtcttcgtg tcgagcggcc
     1201 ccggcgagcc gatgccgacc ccggcgacat cccggccgcg ccctgcgtcg gccatcgatg
     1261 cgcgcgccag atcgacgatc cgcccgacca ccgcgtcggg cccgtcctcg gcctttgtgg
     1321 gctcggacag cacgcccagg atctcggacc cgtcctcggc caccgtgccc gccacgatgt
     1381 tggtgccgcc gacgtcaatc ccgacgatgt atcgcaccgg tttcctccat ggctcgcagg
     1441 acgtcatcca cgctcactgc ctgcagacag cgatggtggc ccagcgggca cgctgcgggc
     1501 ccgtgcggcg agcacgggcg gcaggcgagc ccgtcgagtc ccaccacgac gtcgcgtggc
     1561 ccccgggggc cgaacccgaa cgccggaatc gtcggcccga agatcgccac ggtcggcgtt
     1621 cccaccgcct gggcgaaatg cagcggcgcc gaatcattgg tcacgagaat cgcggcctcg
     1681 cggatcaccg ccgctgattc acgcagcgac aaccggccgc acaggcttac cacgcggtgt
     1741 gcggcgtcct catcgtttgc cgcggccacg atcgcgtcgg cgagcgccgc atcgtcggct
     1801 ccgcccacca gcacgacgtc gcaacgctgc gccagccgcc gcgccagcgc ggcgaagtgc
     1861 ggccatcgct tgctgcccca gatggagccg ggtgcgagcg ccgcaagggg cggcgcgacg
     1921 gccgccgccc ggagccgctc ctcagcccgg gccacatcgc tcgccacgag atgcagcgtc
     1981 ggcagggccc cctggtgcgg cgtcgcctcg accaacgcca gcagccggtc gatctcgtgt
     2041 ccgtcccgcg ggcggggccg cgcttcggtg tacagcgagc gccagccgtc ggtgtagccc
     2101 acgcggcgcg ggatgccggc gagccgcgcc aacgcggcgc tgcgcagcga ccggtgagga
     2161 aggtacgcca ccgcatagcg ttccgcacgc agctggcggg cgagccgccg cagtcccgcg
     2221 aatccgcggt cggccttccg cttgtcgtac gcgagcaccc gtcgcacggc cggatgcgtt
     2281 tcgagcagcg gtgcggcgac g
//
LOCUS       DBNGZW010000251         2356 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_159514, whole genome shotgun sequence.
ACCESSION   DBNGZW010000251 DBNGZW010000000
VERSION     DBNGZW010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2356)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2356)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2356
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_159514"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1182
                     /locus_tag="AC16JC_05990"
     CDS             <1..1182
                     /locus_tag="AC16JC_05990"
                     /EC_number="3.4.16.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S10 family peptidase"
                     /protein_id="HIR5164282.1"
                     /translation="TTDAAPTPPPPYQMVDNASSLIDVADLVFIDPIGTGFSKPVGKG
                     TGKDFWGVDEDARSLAQFVSQYVSRNGRWSSPKYLIGESYGTTRSAVLVNRLQRDGMT
                     FNGVVLISSVLDFETLLFSPGHDLSYVLYLPSYAATAVYHKVIPAPANLDAFLTEVRR
                     YAMGDYADALAAGATLPADRKAAVAKQVAAYTGLSEDYLMRADLRVNLRQFMAELQRS
                     RGLVTGRLDSRFTGPLPDLLSETAPGDPQSSAVSGAFTAATNAYLRGELKFDTQDRYL
                     MGGGVQWNWTRDGQRGWASTTYVGSDLTQALVANPHLRIEIENGYYDLATPFVATEYT
                     VSHLEGLGPDLRDNISLKYYDAGHMMYLHEPELAKLKQNIARFITGGGPAATTAGGGS
                     N"
     gene            1184..2224
                     /locus_tag="AC16JC_05995"
     CDS             1184..2224
                     /locus_tag="AC16JC_05995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HIR5164283.1"
                     /translation="MKVGLVGFAGSGKTTVFNTMTGLAVPTGFGGEVRLGTVKVPDER
                     IDTLSGIFKPKKTTYAEIVFCDVPGEHGAERKGLSRKALQQIRDQEVLCLVLRAFPNP
                     ALEGEPDPAGDLEAFHTECLLADLDLVMRRLDRAKKDKPDPLELTAFELMKATLEQEL
                     PLRSISAQALNRDYLKAYAFLTDRPLMIALNRSEQEATQPLPPALTARLTQLHAAGLV
                     LSASVEAEIAQMDPADRAAFLEDLGLKESALTRFIRTAHGLMDLISFFTVGPDEVRAW
                     PIRRGSTAKEAAGKIHSDLERGFIRAEVIPYAVFMQHGSEHAVKDAGLLQIHGKDYVV
                     ADGDILHIRFNV"
BASE COUNT          396 a          831 c          772 g          357 t
ORIGIN      
        1 accaccgacg cggcgcccac gccgccccct ccctaccaga tggtggataa cgccagcagc
       61 ctgatcgacg tcgccgacct ggtgttcatc gaccccatcg gcaccggctt ctccaagccc
      121 gtcggcaagg gcaccgggaa ggacttctgg ggcgtggacg aggatgcgcg ctcgctcgcc
      181 cagttcgtgt cacagtacgt gagccgtaac ggccgctggt cctcgcccaa gtacctcatc
      241 ggcgagagct acggcaccac ccgctcggcg gtgctcgtga accggctgca gcgcgacggc
      301 atgacgttca acggcgtggt gctgatctcg tcggtgctgg acttcgagac gctgctgttc
      361 tcgcccggcc acgacctgtc ctacgtcctc tatctgccga gctacgccgc gacggccgtg
      421 taccacaagg tgatcccggc gccggccaat ctcgacgcgt tcctcacgga agtgcgccgc
      481 tacgcgatgg gagactatgc cgacgcgctc gccgcgggcg cgaccctgcc cgcggaccgc
      541 aaggcggcgg tcgccaagca ggtagcggcc tacaccgggc tctccgagga ctacctcatg
      601 agggcggacc tgcgggtgaa cctgcggcag ttcatggcgg agctgcagcg tagccggggc
      661 ctcgtcaccg gacggctcga ttcgcgcttc accgggccgc tgcccgacct gctcagcgag
      721 accgccccgg gcgatcccca gtcgtcggcc gtgagcggcg ccttcaccgc ggcgaccaac
      781 gcctacctgc gcggcgagct caaattcgac acccaggatc gctacctgat gggcggcggc
      841 gtccaatgga actggacccg cgacggacaa cgcggctggg ccagcacgac ctacgtcggg
      901 tcggatctga cgcaggcgct cgtcgcgaac ccgcacctgc gcatcgagat cgagaacggg
      961 tactacgacc tcgcgacgcc gttcgtcgcc accgagtaca ccgtgagcca cctcgagggc
     1021 ctggggcccg acctgcgcga caatatctcg ctgaaatact acgacgccgg ccacatgatg
     1081 tacctccacg agccggagct cgcgaagctg aagcagaaca tcgcgcgctt catcacgggg
     1141 ggcggaccgg cggcgacgac ggccggcggg ggatccaact gacatgaagg tcggcctggt
     1201 cggcttcgcg ggttcgggca agacgacggt gttcaatacc atgaccggtc tcgccgtgcc
     1261 caccgggttc ggcggagaag tgcgcctcgg gacggtgaag gtcccggacg agcgcatcga
     1321 cacgctgagc gggatcttca agccgaagaa gacgacgtac gcggagatcg tcttctgcga
     1381 cgtccccggg gagcacggcg ccgagcgcaa ggggctctcg cgcaaggcgc tgcagcagat
     1441 tcgcgaccag gaggtcctgt gcctggtgct gcgcgccttc ccgaaccccg cgctggaggg
     1501 ggagcccgat cccgcaggcg acctggaggc gttccacacc gaatgcctgt tggccgacct
     1561 cgatctcgtg atgcgccgcc tcgatcgcgc gaagaaggac aagcccgatc cgctcgagct
     1621 gaccgcgttc gagctgatga aggcgacgct ggagcaggag ctccccctcc gctccatctc
     1681 ggcgcaggcg ctgaatcgcg actatctcaa ggcgtacgcg ttcctcaccg atcgcccgct
     1741 gatgatcgcg ctgaatcgca gcgagcagga agcgacgcag cccctgcccc cggccctgac
     1801 cgcgcggctc acccagctgc acgccgcggg actcgtgctg tcggccagcg tggaagcgga
     1861 gatcgcgcag atggaccccg ccgaccgtgc ggctttcctc gaggatctgg gactcaagga
     1921 gtcggcgctg acgcgcttca tccggaccgc gcacggcctg atggatctca tctccttttt
     1981 caccgtcggc ccggacgagg tgagggcctg gccgatccgc cgcggctcga ccgcgaagga
     2041 ggcagccggc aagatccact ccgacctgga gcgcggtttc atccgggcgg aggtgatccc
     2101 ctacgccgtc ttcatgcagc acggctccga gcatgcggtg aaagacgccg gcctgctgca
     2161 aatccatggg aaggactacg tggtggccga tggcgatatc ctgcacatcc ggttcaacgt
     2221 gtagccaggg gagtcgagcc gatccgagtc ctcgtcataa tcctcggcgc actggctgtc
     2281 gcattcctcg cgctggccat cccggcaccc gagcctccgc cccctccggc gccggagctc
     2341 ggcgccgcgt tcgagt
//
LOCUS       DBNGZW010000252         3824 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_22501, whole genome shotgun sequence.
ACCESSION   DBNGZW010000252 DBNGZW010000000
VERSION     DBNGZW010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3824)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3824)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3824
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_22501"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="AC16JC_06000"
     CDS             <1..618
                     /locus_tag="AC16JC_06000"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HIR5164284.1"
                     /translation="QFRPIAGVPMLLRALRPFLARRDVGRVVVTLPAGVAEKPPVWLQ
                     DLLGPRVSGVSGGATRADSVRAGLQGLGALPPVVLVHDVARPFVDGATISAVIEHARA
                     GVGAVAAVPMGDTVKDVADDGRVVRTVARIRLWRAQTPQGFPGAMLAEGYARWPADAP
                     PPTDDAEAVERAGFPVEVVAGSAFNLKVTTEDDWRLAEALAREVG"
     gene            615..1268
                     /locus_tag="AC16JC_06005"
     CDS             615..1268
                     /locus_tag="AC16JC_06005"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR5164285.1"
                     /translation="MTAVPFVTLADARAAVPRVKAHLRAGGLLAYPTETVYGLGSRPV
                     DPDLRTLAALKGRAAGKPFLLLVASREMAEAQGLAMNESARALARTFWPGPLTLVLPG
                     GSGRLPDLLRGPEGGIAVRWTSHPGMALLVAALGEPVTSTSANLPGAPPAPGADAILR
                     DFAPAVADGRLLVLDGGVLGNQPPSTVVDCTGPLARVVRSGAIPVARLRAAVGMLAP"
     gene            1265..1741
                     /locus_tag="AC16JC_06010"
     CDS             1265..1741
                     /locus_tag="AC16JC_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HIR5164286.1"
                     /translation="MKRVLFVCTGNICRSPLAEALLKRALAERGLDDVAVASAGTGAW
                     DGAPASEGAYLVALERGLDLSSHRARLLTREIVEDTELILTMARHHRARVHELGGEGR
                     VFVLGEFAGRTGEEAEVGDPFGGDLEVYRDTCAELETLIAAVADRLAGRARSEDRQ"
     gene            1725..2543
                     /locus_tag="AC16JC_06015"
     CDS             1725..2543
                     /locus_tag="AC16JC_06015"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase family protein"
                     /protein_id="HIR5164287.1"
                     /translation="MRIGSNTRLLAVLGDPIAHSLSPIMHNAALDALGVDAVYVALRV
                     SPGVFSDTFRALGTLGVAGNVTVPHKEAAERDVTRKTDLCARAGACNTYWVEQGALVG
                     DNTDVPAIAAELAELGVDGGHWLVVGTGGSARAAALAAADARAELSVHSRDARRASEF
                     AAWASDLGVKARVAKPPYAADVVVNATPLGLASSDPLPLDPEHMRGIRAALDLVYGPG
                     ETRWARELRSRSIATRDGRGVLVRQGALAWERFFPDRPAPVEIMRAAVERALRD"
     gene            2536..3285
                     /locus_tag="AC16JC_06020"
     CDS             2536..3285
                     /locus_tag="AC16JC_06020"
                     /inference="COORDINATES: protein motif:HMM:NF039185.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HIR5164288.1"
                     /translation="MTSALEQLLLPAECLVCHGLLGNAHADDLVCPVCRLRWRPVRPP
                     WCERCGQPEPHFGPCRLCREWPEALRAVRSAVWLDGGAREAVHALKYRGLPRIAVDLA
                     ESLVRLLPRPAPGSRLAPIPLGPARLKRRGYNQSELLARALGRRWGLVVSPQLLVRVR
                     DTPTQTALTPAARLANVAGAFALRIEDCGLRIGSRSQSAISNPQSPIVLVDDVFTTGA
                     TLAEAARVLGEAGFSSITAVTFGRASIPDFS"
     gene            3294..>3824
                     /locus_tag="AC16JC_06025"
     CDS             3294..>3824
                     /locus_tag="AC16JC_06025"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003867202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR5164289.1"
                     /translation="MAVRVGINGFGRIGRNVVRAALLSKQTDLEFVSVNDITDPKTLG
                     HLLKYDSVHGRFPGTVEVKGDALVVNGKEVRVTAIKEPEKLPWKELGVDLVLESTGRF
                     TDRDSAAKHLAAGARKVVISAPAKGEDITIVMGVNEKKYDPAAHHIISNASCTTNCLA
                     PLVKVLHENFTIRKGSM"
BASE COUNT          498 a         1270 c         1462 g          594 t
ORIGIN      
        1 cagttccggc ccatcgccgg cgtgccgatg ctgttgcggg cgctgcggcc gttcctggcg
       61 cggcgtgacg tggggcgcgt ggtggtgacg ttgcccgccg gcgtagccga gaagccgccc
      121 gtatggctgc aggatctgct gggcccgcgc gtcagtggag tgagcggcgg ggcgacgcgt
      181 gccgattcgg tgcgggccgg gctccaggga ctgggcgcgc tgccgcccgt cgtcctcgtg
      241 cacgatgtcg cgcgcccgtt cgtggacggc gcgacgatca gcgcggtgat cgagcacgcc
      301 cgggccggcg tcggcgcggt ggcggccgtt ccgatgggcg atacggtgaa ggacgtggcg
      361 gacgatgggc gggtggtgcg gacggtggcg cggattcgcc tgtggcgggc gcagacgccc
      421 caggggttcc ccggagcgat gctcgccgag gggtacgccc gctggccggc cgatgccccc
      481 ccacccaccg acgatgccga ggcggtggag cgggccggat tccccgtgga ggtcgttgcc
      541 gggtccgcct tcaacctgaa agtgacgacc gaggacgatt ggcgcctggc ggaagccctg
      601 gcgcgggagg tgggatgacc gccgtcccgt tcgtgacgct ggcggacgcc cgggcggcgg
      661 ttccgcgggt aaaagcacac ctgcgcgcgg ggggcctgct cgcgtatcct acggagacgg
      721 tgtacggttt gggatcccgg cccgttgacc ccgacctccg tacactggcg gcgctcaagg
      781 ggcgcgcggc cgggaagccg ttcctgctgc tggtggccag ccgcgagatg gccgaggcgc
      841 aggggctcgc catgaacgaa tccgcccgcg cgctggccag gacattctgg cccggaccgc
      901 tgacgctggt gctgccgggc ggtagtggac ggttgcccga cctgctgcgt gggccggaag
      961 gaggtatcgc cgtgcgctgg acatcgcatc ctggaatggc gctgctcgtg gccgcactgg
     1021 gtgagccggt gacgtccacg tcggcgaacc tgcccggcgc gccccccgcg cccggggcgg
     1081 atgccatcct gcgcgacttc gccccggccg tcgccgacgg gcggctgctg gtgctcgatg
     1141 ggggcgtgct gggcaatcag ccgccttcga cggtggtgga ctgcaccggc ccgctcgctc
     1201 gagtggtgcg gtccggggcc atcccggtcg cgcggctgcg cgcggcggtg gggatgctgg
     1261 cgccgtgaag cgcgtcctct tcgtctgcac cggaaacatc tgtcgcagcc cgctcgccga
     1321 ggcgctgctg aagcgggcgc tcgccgagcg gggactggac gacgtcgccg tggcgtcggc
     1381 cggcacgggg gcctgggacg gtgcgcctgc ctccgaaggg gcctatctgg tggccctgga
     1441 gcgcgggctc gatctctcga gtcatcgcgc gcgcctgctc acgcgcgaga tcgtggagga
     1501 cacggagctg atcctcacga tggcgcggca ccaccgcgcg cgggtccacg agctcggcgg
     1561 tgaggggcgg gtcttcgtgc tgggcgagtt cgcgggacgg accggggagg aggccgaggt
     1621 gggcgatccg ttcgggggag acctcgaggt gtatcgcgac acctgcgccg agctcgagac
     1681 gctcatcgcg gcggtggccg atcgcctcgc aggcagggca cggagtgagg atcggcagta
     1741 acacccggct gctcgccgtg ctgggcgatc ccatcgccca ttcgctgtct cccatcatgc
     1801 acaacgccgc cctcgacgcg ctgggagtgg acgcggtgta cgtcgccctg cgcgtttccc
     1861 ccggcgtctt ctccgatacg tttcgcgccc tcggcacgct cggcgtcgcc ggcaatgtca
     1921 cggtcccgca caaggaagcg gcggaacggg acgtgacgcg taaaacggac ctgtgcgccc
     1981 gggccggcgc atgcaatacc tactgggtgg aacagggcgc cctcgtcggc gacaacaccg
     2041 acgtccccgc catcgcggcc gagctggccg agctcggggt cgacggcggg cactggctgg
     2101 tggtgggtac gggcggctcg gcacgggccg cggcgctcgc cgcggccgat gcccgcgccg
     2161 agttgtcggt gcattcacgc gacgcccgca gagcgagcga gttcgccgcc tgggcgtccg
     2221 atctcggggt gaaagcgcgg gtggcgaagc ccccgtatgc ggccgatgtg gtcgtgaacg
     2281 ccaccccgct gggtctcgcc tccagcgacc cgctccccct cgacccggag catatgcgcg
     2341 gtattcgggc ggccctcgat ctggtgtacg gccccgggga aacccgctgg gcccgcgagc
     2401 tgcgcagccg ttccatcgcc acgcgcgacg ggcggggagt gctggtgcgc cagggggcgc
     2461 tcgcctggga gcggttcttt cccgaccggc cggcgccggt cgagatcatg cgcgcggcgg
     2521 tcgagcgcgc gctccgtgac tagcgcgctc gagcagctgc tcctcccggc cgagtgcctg
     2581 gtgtgtcacg ggctgctcgg gaacgctcac gccgacgacc tcgtctgtcc cgtgtgccgc
     2641 ctgcggtggc gtcccgttcg cccgccctgg tgcgagcgct gcggccaacc cgagccccat
     2701 ttcggtccct gccggctctg tcgcgaatgg ccggaggcgc tgcgcgccgt ccgctcggcc
     2761 gtctggctgg atggcggcgc ccgggaggcg gtgcatgcgt tgaagtatcg gggactgccg
     2821 cgcatcgcgg tcgacctggc cgagtcgctc gtccgcctgc tgccgcgccc ggccccggga
     2881 tcgcggcttg cgccgatccc gctcgggccc gcgcggttga agcggcgtgg ttacaatcag
     2941 agcgagctgc tggcgcgggc gctggggcgc cggtggggcc tggtggtgtc accacaactc
     3001 cttgtcagag tgcgtgatac gccgacgcag acggcgttga caccggcggc gcgcctggcg
     3061 aacgtggcgg gggcgtttgc attgcggatt gaggattgtg gattgcggat tggcagccga
     3121 agtcaatccg caatcagcaa tccgcaatcc ccaattgtct tggtagacga cgtgttcacc
     3181 accggcgcga ccctggctga ggcggcgcgg gtgctgggtg aggcgggttt ctcttcgatc
     3241 acggccgtga cgttcggccg cgcgagcatt ccggacttct cttaggagcg aacatggcgg
     3301 tgcgggtggg catcaacggc ttcgggcgca tcggccgcaa cgtggtgcgg gcggcgctct
     3361 tgtccaagca gaccgacctg gagttcgtga gcgtcaacga catcacggat cccaagaccc
     3421 tcgggcacct gctcaagtac gactcggtgc acgggcggtt cccgggaacg gtcgaggtga
     3481 agggggatgc gctcgtcgtg aacgggaagg aggtccgggt cacggcgatc aaggagccgg
     3541 agaagctccc ctggaaggag ctgggtgtgg atctcgtgct cgagagcacg gggcgattca
     3601 ccgatcgcga ctcggccgcc aagcacctcg ccgccggcgc caggaaggtg gtgatctccg
     3661 ctcccgcgaa gggcgaggac atcacgatcg tgatgggcgt gaacgagaag aagtatgatc
     3721 cggccgccca tcacatcatc tccaacgcct cctgcaccac caactgcctg gccccgctgg
     3781 tgaaggtgct gcacgagaac ttcaccatcc ggaagggctc gatg
//
LOCUS       DBNGZW010000253         8978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_840179, whole genome shotgun sequence.
ACCESSION   DBNGZW010000253 DBNGZW010000000
VERSION     DBNGZW010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8978
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_840179"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="AC16JC_06030"
     CDS             complement(<1..530)
                     /locus_tag="AC16JC_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164290.1"
                     /translation="MSNRTMVLLTLAGTLSSSDALAQRAPLRSTPTPRDIVARAVAGM
                     GGEQALRGVRALTTEFYGVTFALGQEETPGSPARATATVARIVADYPGGRRLSTLESR
                     GVQGQVNRVRRVIQSDIGMVETDGRPAAASPTLVRTEQASLRRAPERLLLAALDEPAA
                     LRPLAPKSWRGEPMDGV"
     gene            complement(559..1443)
                     /locus_tag="AC16JC_06035"
     CDS             complement(559..1443)
                     /locus_tag="AC16JC_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5164291.1"
                     /translation="MTRTALVLLAALASVPPLAAQGPPGTDIWVADLTLRDGRVALGT
                     PGNLTARAGYDNQPAFLPDGSGLLYTRIGADAQADVWRYAFATRSTRALITTPESEYS
                     PTPIPDGGISVVRVERDSTQRLWRFDADGTNPALLLERLKPVGYHAWTGDRTLALFVL
                     GTPNALVLSTASSERADTVARDIGRSPQSVPGRHAATFVQRVDSTESWLAEVDGETRA
                     VRRLVRLPRGADFHSWTPGGLVLTSDGATLYQWDSHAAGPWVPVADLSTLGLHEVTRL
                     AVSPGGDRLALVAREPAP"
     gene            1525..4080
                     /locus_tag="AC16JC_06040"
     CDS             1525..4080
                     /locus_tag="AC16JC_06040"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5164292.1"
                     /translation="MTGVEGLRGALVGRYDIEREVGHGAMSTVYLARDLRYDRRVAVK
                     VLSRELAAALGPERFLREIDILAHLNHPHILPLLDSGDAGGFLFYVMPFVEGESLRHR
                     LERETQLPIPDALAITREVALALDYAHRQGFIHRDVKPENILLSDGLALVADFGIARA
                     INQAADSDSLTGSGRSPGTPPYMSPEQASGGEVDSRCDIYALGCVLYELLAGQPPFPG
                     PTAQAVLARHLADPVPPLRTVRTTVPLGVERAVFKALAKVPADRFATAGEFAAALAAP
                     DDREGPNWRRSIAISSLVGAVAVIVWLASRNWPAGGAGAAVDTTFYAVFPFERDSGLA
                     SFNEDQLLQDAMSQWTGVGVVDRPRLSEALPRNHARLTVAEAAAAAERVGAGRYVLSQ
                     MSRVGDSLRIHSAVYATTARGPPIHEGTTKVGSNVGQDGPAFARIADHLLFGNAGPSA
                     QLDLPTGTASRPARQSFEHGLDSVYTWNLSAADSAFNRATRYDPEYAPALLWLALVRA
                     WMGTPLPTWQSAAERAAARQVRLSMRDHLISDALVALARGQPERACDTWSRVAAQAPD
                     DFAAWYGLANCLSNDRGVRRDRASPSGWRFRTSYYRATKAYQRAFLLLPYIHKALSPG
                     QYASVRRLLWTNGSELRTGYSPPPDTTTFAAYPTWQGDSLAFVPIPLGMLAGPDVVPA
                     GMRTAVQRERRLFHDIAIQWVSAFPQSAEALEALAISLELLGEPAALDTLRRARALAT
                     TPEARVRLAGSQVWMLVKFSAPSDTAGLRAARILADSVLQGAWVPSAKQSILLGSLAA
                     LTGRGRLAAELSRDPAFINEWEVPAAIQKTGGPLLAFAALEVPQTVSGTSNGR"
     gene            4131..4631
                     /locus_tag="AC16JC_06045"
     CDS             4131..4631
                     /locus_tag="AC16JC_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164293.1"
                     /translation="MQWLGRPAALGFPHYRFGSLESLAAHGNHLVDAEVAFLRGDTDA
                     VRQIFDDLKAARSSALAQDLTFDALYPEAWLLRSLGDAAGAIAWLDPTLATLSASTPE
                     KFADLANAGALVQAMALRAELADQVGDARTAARWARVVTILWSDADPFLQPVVRRMEQ
                     LSRGVL"
     gene            complement(6046..6678)
                     /locus_tag="AC16JC_06050"
     CDS             complement(6046..6678)
                     /locus_tag="AC16JC_06050"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HIR5164294.1"
                     /translation="MPPPLITITRQYASGGSDIAQGVAAALKWTLIDNEFVDSVARRA
                     GLPAAEVAQREERAPGLLERLARTLAIASHELFTGPAVSDAERDEATIHRLTERIIHE
                     AAALGRVVLVGRGAQAILAQRPDALHVYVVGSKPWRVRFAVERLGIEPAKAEKLVEET
                     DRQRDDYVKTYYGRARREAANYDLVVNAERLGLDGAAALVVAEARRRGWE"
     gene            complement(6783..8267)
                     /locus_tag="AC16JC_06055"
     CDS             complement(6783..8267)
                     /locus_tag="AC16JC_06055"
                     /EC_number="6.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015995 - chlorophyll biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase ATPase subunit I"
                     /protein_id="HIR5164295.1"
                     /translation="MSAAKPATLGELKQTEWASPARIHRTVKDELRENLICLLENGSP
                     LFPGILGYEETVVPQLVNAVLSRHNFILLGLRGQAKSRIVRQLVTLLDEEIPFLAGSE
                     VNDHPFAPISKYGRELVAERGDRTPIAWLPRDGRFVEKLATPDVTIADIIGDVDPIKA
                     ARGGHLLSDELTMHFGLLPRANRGIFAINELPDLAGKIQVGLFNIMQEGDVQLKGYPV
                     RLNLDVALVFTANPEDYTARGKIITPLKDRIGSEIMTHYPHTVELGATITEQEAWLTR
                     NGRPIRVPDFIAELVERIAFGARADKRVDKKSGVSQRLPISVLENVVSNAERRAIVTK
                     EKAVVPRVSDVYAALPAITGKLELEYEGELQGADTVAKDLIKRAAGDTFEARVGGANV
                     DDIVHWFDEGGALKIATDERSEALLKGFSVVPGLLDLVDRVGLAGKKDPAHMVAACEL
                     VLEGLVALRRISRSEDLGYVRARPEPRNPGYGKPGPGAGPNLFT"
     gene            complement(8277..>8978)
                     /locus_tag="AC16JC_06060"
     CDS             complement(8277..>8978)
                     /locus_tag="AC16JC_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164296.1"
                     /translation="RPTVELEVPLGRMTVERDQQPGSCRDWYCHTHWVWLHEATSGVL
                     WSSPDAPLVTLTDLFRGAWRRTITPDGTLFSWVLNNYWHTNYAARQGGDLVFRYRLSL
                     LAPGDAAEPVRRGWAACEPLYVGPPYTNVTPGPLVSRDSALSVPDRGVAVIGVKPADD
                     DEGAIVKLLDVRGTRRSVGMWPAAYAFQQARRVNLVEMNGAPLTVNADRSVNVEVAAW
                     GIAAARLFTPRETAG"
BASE COUNT         1418 a         2954 c         3088 g         1518 t
ORIGIN      
        1 accccgtcca tcggctcgcc ccgccatgac ttcggcgcga gcggccgcag cgcggccggc
       61 tcgtcgagcg cggccagcaa cagacgctcg ggcgcgcggc gcaggctggc ctgctccgtc
      121 cgcaccaggg tgggactggc ggccgccggc cggccgtcgg tttcgaccat cccgatgtcg
      181 ctctggatca cgcggcgtac gcggttgacc tgcccctgga cgccacggct ctccagcgta
      241 ctgaggcgtc gtccgcccgg gtagtcggcg acgatccgcg cgacagtggc ggtggcacgc
      301 gccggggagc ccggcgtttc ctcctggccc agcgcgaacg tcacgccgta gaactcggtc
      361 gtcagcgcgc ggacgccgcg caacgcctgc tcgccgccca tcccggcgac ggcccgggcg
      421 acgatgtcgc gcggcgttgg ggtggagcgg agcggggcgc gctgggcgag cgcgtcactg
      481 gaggagagcg tccctgcgag cgtgaggagg accatcgtgc ggttgctcat gtgaatcctc
      541 gtaccgatgg agggtcgatc agggcgcagg ctcccgggcc accagcgcca gccggtcgcc
      601 gccggggctg accgcgagcc gggtcacctc gtgcaggccg agggtcgaaa gatcggcgac
      661 gggcacccag ggtcctgccg cgtgcgaatc ccactgatac agcgtcgcgc cgtcgctcgt
      721 cagtaccagc ccgccgggtg tccaggagtg aaagtccgcg ccgcggggga gccgcaccag
      781 gcgccggact gcgcgggtct cgccgtccac ttccgccagc catgactccg tcgaatccac
      841 gcgctgtacg aaggtcgcgg cgtggcggcc ggggacggac tgcggtgagc ggccgatgtc
      901 ccgggccacc gtgtcagcgc gctcgctgct ggcggtcgag agcaccagcg cattgggagt
      961 gccgaggacg aacagggcga gggtgcggtc gccggtccag gcgtgatagc cgacgggttt
     1021 gagccgctcg agcagcagcg ccgggttggt gccgtcggcg tcgaagcgcc agaggcgttg
     1081 ggtcgagtcg cgctcgacgc ggacgaccga gatcccgccg tccgggatgg gcgtgggtga
     1141 gtattcgctt tcgggcgtgg tgatgagcgc gcgggtcgag cgcgtggcga aggcgtagcg
     1201 ccacacgtcc gcttgcgcgt ccgcgccgat gcgtgtgtac aggagccctg agccgtcggg
     1261 caggaacgcc ggctggttgt cgtagcccgc acgggcggtg aggttccccg gcgtcccgag
     1321 ggccacgcgg ccgtcgcgca gagtgagatc ggccacccag atatcggtac caggcggccc
     1381 ctgagcggcg agcggtggga ccgaggcgag cgcggcgagc agcaccagag cagtccgggt
     1441 catgggcggg aacgtacacg ggagaacgct ttggcgcgaa ggctctaacg gactccgggt
     1501 tttgcggcat cttgtccggg ggctatgacc ggagttgagg ggctccgggg cgcgctcgtt
     1561 ggccggtacg acatcgagcg cgaggtgggg cacggggcga tgtcgaccgt gtacctggcg
     1621 cgggacctgc gctacgaccg gcgcgtcgcg gtgaaggtgc tgagccgcga gctggccgcg
     1681 gcgctcggcc cggagcgctt tctgcgcgag atcgacatcc tcgcgcatct caaccatccg
     1741 cacattctgc cgctgctcga ctccggtgac gccggaggat ttctctttta cgtgatgccg
     1801 ttcgtcgagg gcgaatcgct gcgccaccgg ctggagcggg agacgcagct cccaatcccc
     1861 gatgcgctcg cgatcacgcg cgaggtggcg ctggcgctcg actacgcgca ccgtcagggg
     1921 ttcatccatc gtgacgtgaa gcccgagaac attctgctct ccgacggcct ggcgctggtg
     1981 gccgacttcg gcatcgcgcg cgcgatcaac caggccgcgg actcggacag cctcacgggg
     2041 agcgggcgct cgccggggac gccgccctat atgtcgccgg agcaggcgtc gggcggggag
     2101 gtggacagcc gctgcgacat ctatgcgctg gggtgcgtgc tgtacgagct cctggcgggg
     2161 cagccgccgt tcccgggtcc gacggcgcag gcggtgctgg cccggcatct ggccgatccg
     2221 gtgccgccgt tgcgcacggt gcggacgaca gtgccgctcg gggtggagcg ggcggtgttc
     2281 aaggcgctcg cgaaggtacc ggcggaccgg ttcgcgacgg cgggcgaatt cgccgcggcg
     2341 ctggcggcac ccgatgaccg ggaggggcca aactggcgtc gctcgatcgc gatctcgtcc
     2401 ttggtggggg ccgtagcggt gatcgtctgg ctcgcgagcc gaaactggcc cgcgggcggc
     2461 gcgggggcgg cggtggatac gaccttctac gcggtctttc cgttcgagcg cgactccggc
     2521 cttgcctcgt tcaacgagga tcagctactg caggacgcga tgtcgcagtg gaccggcgtc
     2581 ggcgtggtgg accggccccg cttgtcggaa gcgctaccac gcaatcacgc gcgactcacg
     2641 gtcgcggagg cggcagcggc tgccgagcgc gtaggggcgg ggcgctatgt cctgagtcag
     2701 atgtcgcggg tcggcgattc gctgcggatc cattcggcgg tatacgccac gaccgcgcga
     2761 gggcccccga ttcacgaggg caccaccaaa gtgggctcga acgtcgggca agatggtccg
     2821 gcgttcgccc ggatcgcaga tcatcttctg ttcggcaacg ccgggccaag cgctcaactg
     2881 gacctgccga cggggacggc atcacgaccc gcacgtcagt cgttcgagca cgggctggac
     2941 tccgtctaca cctggaacct gtcggcggca gactcggcgt tcaatcgcgc gacccgatac
     3001 gaccctgaat acgcgcctgc gttgttgtgg cttgcgttgg tacgagcatg gatgggcacg
     3061 ccgctcccca cgtggcagtc cgccgcagag cgtgcggctg cgcgtcaagt gcggctctcg
     3121 atgcgtgatc acctcatttc ggatgcactg gttgccttgg ccaggggaca acctgaacgc
     3181 gcttgcgata cgtggtcgcg cgtggcggcg caagcgccgg acgatttcgc agcatggtac
     3241 ggattggcca attgtctgag caacgatcgg ggcgtccgtc gggaccgggc aagtccgagt
     3301 gggtggcgct ttcgcaccag ttactatcgg gcgaccaagg cataccagcg ggcgttcctg
     3361 ctcctgccgt acatccacaa agcgctcagc cctggacagt atgcgtcggt gcgaaggtta
     3421 ctctggacga acggcagcga actgcgaacc ggatattctc cgccgccgga cacaacgacg
     3481 tttgccgcgt atcccacttg gcagggggac tcgttggcat ttgtgccgat tccgcttggt
     3541 atgttggccg gccctgatgt ggtgccagca gggatgagaa ctgctgtgca gcgcgaacgg
     3601 cgattgtttc atgacatcgc gattcagtgg gtgagtgcat ttcctcagag tgctgaggct
     3661 ctggaagcgc tcgcgatatc gctagagttg ttgggtgaac cggctgctct cgatacgctg
     3721 cgtcgggcac gggcgcttgc taccacaccc gaagcgcggg tccgtcttgc aggcagtcag
     3781 gtctggatgc tcgtgaagtt ctcggcgccg tccgatacgg ctggcttacg cgccgcccgg
     3841 atcctggcag actccgtact tcaaggcgct tgggttccgt ccgccaaaca gtcgatcctc
     3901 ttggggagtc tcgccgcact caccggccgt ggccggttag cggccgaact gagccgggat
     3961 cctgcgttca ttaacgagtg ggaggtacct gcggccatcc aaaagacggg gggcccgctt
     4021 ctagcctttg cggccctcga ggtcccacag acagtctctg ggacatcgaa cggcaggtaa
     4081 acgacgctat cgagaaagag ctcttcggcg ctgtacagca agacgagcgc atgcagtggc
     4141 ttgggcggcc ggcggcccta gggtttccgc attaccggtt cggttcgctc gagagccttg
     4201 cagcacacgg gaaccatctg gtcgacgccg aggtggcttt cctccggggc gataccgatg
     4261 cggtgcggca gatattcgat gacctcaagg cagcgcgtag ctcagcactg gcgcaggacc
     4321 tcacattcga tgccctctat ccggaggcgt ggctcctgcg gtcgctgggg gatgcagcgg
     4381 gggcgatcgc ctggctcgat cccacgttgg cgacgttgtc ggccagtacg cccgaaaagt
     4441 tcgccgacct ggccaacgcc ggcgcgctcg tgcaggcgat ggcactgcga gcggaactcg
     4501 ccgaccaagt tggggatgct cggacggcgg cacgctgggc gcgcgttgtc accatccttt
     4561 ggagcgatgc tgatcccttt ttgcagccag tcgtccgtcg aatggagcaa ttatcgcgag
     4621 gagtcctgtg agttgcagag tcgtctggct cgaacgggag ggagacgatc atgtccacgc
     4681 cgtctaaccg tatcggtgtc aagctggccg caaccgcctc agtcgcggtt ggtcttgcgt
     4741 actgcagagc tgctgcaaga agaaacaaac gccggagcct gcaacgaaga tcgccgcttg
     4801 gcgtttcgaa tgtcccgagc atggcgtggc tattcgaggc gttgatacgg ccggggtgac
     4861 cgtcgactcc atctttcaga ttccgctctc cgagccgata accaacattc ccgagtatca
     4921 cgactgtcag cgattcattg attccactgg gtacggctcc gtctatgcag tattcgccgc
     4981 atttcggctc gacctgattg ctggaggacc ccagccgacg cctctagcga cgatctacac
     5041 cccggatggt gagtatccga ctttgggaat caagccaggc ttcaactgcc ttgtgatgtc
     5101 caggacgggg aaggtgtgga aagcccaaat gatctggaga ggtcagggtg aggacaaggc
     5161 gaactgcgcc gccaagcctt tgactgggag ccaagagaca acgctcgatg ccaaaccaca
     5221 accggttggg aagccgtttg cgccgaacag ctttccgcca gcggctcgtt gggactggga
     5281 ctcactgaac gggaagcagt acatcgggat ccggtgtggg gcggcgtggt gcgaagtcgg
     5341 aactccaggc ttccaggcat cgaccccctt cgagggcccg gtgccgccgt ttgacccaat
     5401 cgctggaatc aacgtgcctg cggaggcgac tgagagagtc cagcgaatca aagggtggta
     5461 tgatgttcag cggttagcac tgtggccgaa cggctcaaac cagcaaccca gcactgtgcg
     5521 aggggttctg attccacatc cggccttgga tatgatcaac tggggagcgg atccgctgca
     5581 gatctacagg gaccggtggg tgcacgtggc atgggccatc gttgatggtg attatcccaa
     5641 gtggcacttc aagcgcggta ataacaagat ctccctgtgt tatggcacag cggactcgac
     5701 gagctgcaac attcccctgt caagccaccc ggagaactcg tcctccatgc cactgggcgc
     5761 ctgtcccacg gatcctgcag ggtctggctt gcgttggtgg tcgaggaccg aatccgccgc
     5821 cgacagcatg agctacgggt gtgttctgag aatgaatcac aaggcggacc tagacgagtg
     5881 gaggaagaca aacccgggcg tgctgttccg tatcccaggt accgctcgct ggaagttcct
     5941 tcccgacgat gagagcacgt gggtcagttg cccaacaggc tgttgcacga aacgctgaaa
     6001 tcgcctggct aaggcatcaa tagccaatag ggagggtggt gggtgctact cccaccccct
     6061 cctcctcgcc tccgccacca cgagcgccgc cgccccgtcc agccccagcc gctcggcgtt
     6121 caccaccaga tcgtaattcg ccgcctcccg gcgcgcccgc ccgtagtacg tcttgacgta
     6181 gtcgtcgcgc tggcgatcgg tctcctccac cagcttctcg gccttcgccg gctcgatccc
     6241 cagccgctcc actgcgaacc gcacccgcca cggtttcgat cccacgacgt acacgtgcag
     6301 tgcgtcgggc cgctgggcca gaatcgcctg cgcgccgcgt cccaccagca ccacccgccc
     6361 cagcgccgct gcttcgtgga tgatccgctc cgtgagccgg tggatcgtcg cctcgtcgcg
     6421 ctccgcgtcc gacaccgcgg gcccggtaaa caactcgtgc gacgcgatcg ccagcgtccg
     6481 cgccagccgc tccagcagcc ccggcgcccg ctcctcgcgc tgcgcgacct ccgcggcggg
     6541 tagccccgcg cgccgggcca cggagtccac gaattcgttg tcgatcagcg tccacttcag
     6601 cgctgcggcg acgccctggg cgatgtcgga gccgccggag gcatactggc gggtgatggt
     6661 gatgagcggg ggaggcattc gactgaaata gaactgcgct gtcggccatc ggctatcggc
     6721 tatcggctgt cggctatcag ctcagccgat agctgatagc cgatagctgg tagccccgcc
     6781 gtctacgtga agagattcgg ccccgctccc ggtcccggtt tcccatatcc ggggtttctc
     6841 ggctccgggc gcgcccgcac atagcccagg tcctccgagc gggagatgcg gcgcagcgcc
     6901 acgaggccct cgagcaccag ctcgcacgcg gcgaccatgt gcgccgggtc cttcttgccc
     6961 gccaggccga cccggtccac gaggtcgagc agccccggca cgacgctgaa tcccttgagc
     7021 aacgcctcgg agcgctcgtc ggtcgcgatc ttcagggcgc cgccctcgtc gaaccagtgc
     7081 acgatgtcgt ccacgttcgc cccgcccacc cgcgcctcga acgtatcgcc cgcggcgcgc
     7141 ttgatcaggt ccttggcgac ggtatcggcg ccctgcagct cgccctcgta ctccagctcc
     7201 agcttaccgg tgatcgccgg gagcgcggcg tacacgtcgg agacgcgcgg cacgacggcc
     7261 ttttccttcg tgacgatggc ccgccgctcg gcgttggaga cgacgttctc gagcacgctg
     7321 atcggcagcc gctgcgacac gccgctcttc ttgtccaccc gcttgtcggc gcgcgcgccg
     7381 aaggcgatgc gctccaccag ctccgcgatg aagtcgggca cgcggatcgg gcggccattg
     7441 cgcgtcagcc acgcttcctg ctcggtgatc gtggccccca gctccaccgt gtgggggtag
     7501 tgggtcatga tctccgagcc gatgcggtcc ttgagcggag tgatgatctt gccccgcgcg
     7561 gtgtaatcct cggggttcgc cgtgaacacg agcgccacgt cgaggttcag ccgcaccggg
     7621 tagcccttca gctggacgtc cccctcctgc atgatgttga acagccccac ctggatcttc
     7681 ccggccaggt cgggcagctc gttgatcgcg aagatgcccc ggttggcccg cggcagcagg
     7741 ccgaaatgca tggtcagctc gtcggagagc aggtgcccgc cgcgcgccgc cttgatcggg
     7801 tccacgtcgc cgatgatgtc ggcgatggtg acgtcgggag tcgcgagctt ctccacgaag
     7861 cgcccgtccc gcggcagcca cgcgatgggg gtgcggtcgc cccgctcggc caccagctca
     7921 cggccgtact tcgagatcgg cgcgaaggga tgatcgttca cctcgctgcc ggcgaggaac
     7981 gggatctcct cgtccagcag cgtcacgagc tgccgcacga tgcggctctt cgcctgcccg
     8041 cgcaggccca gcagaatgaa gttgtgccgc gacaggacgg cgttcacgag ctgcggcacc
     8101 acggtctcct cgtagcccag gatgcccgga aacaacggag acccgttctc cagcaggcag
     8161 atcaggttct cgcgcagctc gtccttgacg gtgcgatgga tgcgggccgg ggaggcccac
     8221 tcggtctgtt tcagctcacc cagggtggcc ggtttggcag cgctcatggg cggacgttaa
     8281 cccgccgtct ctcgcggtgt aaagagccgg gcggcggcga tgccccacgc cgccacctcc
     8341 acgttgacgc tgcgatcggc attcacggtg agcggcgcgc cgttcatctc caccaggttg
     8401 acgcgccgcg cctgctggaa ggcgtacgcg gcgggccaca tgccgacgct gcgtcgcgtg
     8461 ccgcgcacgt ccagcagctt gacgatcgcc ccctcgtcgt cgtcggccgg cttgacgccg
     8521 atcaccgcca caccccggtc gggaacggac agggccgaat cgcgggagac gagcgggccg
     8581 ggcgtgacgt tggtgtaggg cggaccgacg tacagcggct cgcacgccgc ccagccgcgc
     8641 cgcaccggct cggcagcgtc ccccggcgcg agcagcgaga ggcggtagcg gaacaccagg
     8701 tcgccgccct gccgggccgc atagttggtg tgccagtagt tgttcaggac ccaggagaac
     8761 agcgtgccgt ccggcgtgat ggtgcggcgc caggcgccgc gaaacaggtc ggtgagagtc
     8821 acgagcgggg cgtcgggcga gctccacagc acgccggacg ttgcttcgtg caaccacacc
     8881 cagtgggtgt ggcaatacca gtcgcggcag ctcccgggtt gttggtcgcg ctccacggtc
     8941 atccgcccca gcggcacctc cagctcgacc gtgggccg
//
LOCUS       DBNGZW010000254         1534 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_22619, whole genome shotgun sequence.
ACCESSION   DBNGZW010000254 DBNGZW010000000
VERSION     DBNGZW010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1534)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1534)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1534
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_22619"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            5..427
                     /locus_tag="AC16JC_06065"
     CDS             5..427
                     /locus_tag="AC16JC_06065"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007414895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR5164297.1"
                     /translation="MTGARRPARFTREFAVAPGDIDELGHVNNVVYLRWVQEIAVAHW
                     RAAAPAELQAEVVWVVLRHEIDYKRPAHTGEDVVGRTWVGEATGSRFERFTEFARAAN
                     GEVLAQARSVWCPLNAHTGRPRRIDPALHDYFSEPASP"
     gene            495..1394
                     /gene="argF"
                     /locus_tag="AC16JC_06070"
     CDS             495..1394
                     /gene="argF"
                     /locus_tag="AC16JC_06070"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012471179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR5164298.1"
                     /translation="MPKRDFLAVTDLTRDEIGRLFDLADRMKRRQYRETPLAGKTLAM
                     IFAKSSTRTRVSFEAGAFQLGGHALFLSSRDIQIGRGEPIPDTARVLSRYVDGIMIRT
                     FDHGEVEELARCATIPVINGLTDLSHPCQVLADLFTVREALGGWEGKRVAWIGDGNNM
                     ANSWLEAADVLGFELRLACPEGYEPNREKFERAARNARVTVTEVPEEAVEGAHVVNTD
                     VWASMGQETEAEARRNAFRGYTVDAELMKLADPGAIFLHCLPAHRGEEVSAAVIEGRQ
                     SRVWDEAENRLHVQKALLATLMG"
     gene            1405..>1534
                     /locus_tag="AC16JC_06075"
     CDS             1405..>1534
                     /locus_tag="AC16JC_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015452307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR5164299.1"
                     /translation="MSDTMPSTALKRTPLHDLHVALGAKMVPFAGFEMPVTYPAGIG"
BASE COUNT          251 a          517 c          538 g          228 t
ORIGIN      
        1 tcctgtgacc ggagcgcggc ggccggcgcg cttcacgcgt gagtttgcgg tcgccccggg
       61 cgacatcgac gagctgggcc acgtgaacaa cgtcgtctac ctgcgctggg tgcaggagat
      121 cgccgtggcc cactggcgtg ccgccgcgcc ggccgaactc caggccgagg tggtctgggt
      181 ggtgttgcgg cacgagatcg actacaagcg cccggcgcac accggcgagg acgtggtcgg
      241 acgcacctgg gtgggtgaag cgaccggctc ccgcttcgag cgcttcacgg aattcgcgcg
      301 cgccgcgaac ggggaagtcc tggcgcaggc ccgcagtgta tggtgcccgc tcaacgcgca
      361 caccggccgg ccacgccgca tcgatcccgc actgcacgac tacttctccg agcccgcctc
      421 gccttgacgg gcctcgtccc ccgaaggtaa agtgtgcaga cgcgaactgt cattttgacc
      481 tgccccggga agccatgccg aagcgcgact ttctagccgt tacggacctc acgcgggatg
      541 agatcggccg gctgttcgat ctggccgacc ggatgaagcg ccgccagtac cgcgagacgc
      601 cgctcgccgg caagacactg gcgatgatct tcgccaagtc gagcacgcgg acgcgggtct
      661 ctttcgaagc tggcgccttc cagctgggcg ggcacgcgct gttcctctcc tcgcgcgata
      721 tccagatcgg gcggggggag ccgatccccg acacggcccg ggtgctgtcg cgctacgtgg
      781 acggcatcat gatccgcacc ttcgatcacg gggaggtgga ggagctggcg cgctgcgcca
      841 cgatcccggt gatcaacgga ctgaccgatc tgtcgcaccc ctgccaggtt ctggccgacc
      901 tcttcacggt gcgggaggcg ctgggcggct gggagggcaa gcgggtggcc tggatcgggg
      961 acggcaacaa catggcgaac agctggctcg aagccgccga cgtcctcgga ttcgagctgc
     1021 ggctcgcctg tcccgaaggg tacgagccca atcgcgagaa gttcgagcgc gccgcccgga
     1081 acgcccgcgt caccgtgacg gaagtgcccg aggaggcggt cgaaggcgcg cacgtcgtga
     1141 acacggacgt ctgggcgtcg atgggacagg agacggaggc ggaggcccgc cgcaacgcgt
     1201 tccgcggcta caccgtggac gccgagctga tgaagctcgc cgatccgggc gccatcttcc
     1261 tgcactgtct gcccgcccat cgcggcgaag aagtctccgc cgcggtcatc gagggccggc
     1321 agtcgcgcgt gtgggacgag gcggagaacc ggctgcacgt tcagaaagca ctgctcgcca
     1381 ccctgatggg atgagaccgg aaccatgagc gatacgatgc cgtccaccgc gctcaagcgc
     1441 actccgctcc acgacctgca cgtggcgctg ggagcgaaga tggtgccctt cgccgggttc
     1501 gagatgcccg tgacctatcc ggccggcatc ggcg
//
LOCUS       DBNGZW010000255         3372 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_698361, whole genome shotgun sequence.
ACCESSION   DBNGZW010000255 DBNGZW010000000
VERSION     DBNGZW010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3372)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3372)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3372
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_698361"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /locus_tag="AC16JC_06080"
     CDS             complement(<1..583)
                     /locus_tag="AC16JC_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5164300.1"
                     /translation="MTDAQATPVEHIHTTREEAVGIITMDRPERFNAMDVVMAQDFRK
                     AGIQLARDDTIRCVVLRGVEGVFSSGADLKYVRARGDETDTGYLHPEGTGPRKSYGES
                     FKQILEYIHSTISEIKRAPKPFVAAVDGVAAAGGFGIAMACDLVMASERASFEWAYHK
                     TGLTGAESSTFFLPRLVGLRKAMELVLLNPRLSA"
     gene            complement(583..1320)
                     /locus_tag="AC16JC_06085"
     CDS             complement(583..1320)
                     /locus_tag="AC16JC_06085"
                     /inference="COORDINATES: protein motif:HMM:NF012760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016742 - hydroxymethyl-, formyl- and
                     related transferase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltransferase family protein"
                     /protein_id="HIR5164301.1"
                     /translation="MLSRSPRVAVLSTTRAPGLDYLLERRQLVAFIATDPANRDLVQV
                     TEGGVPAAVRDIRAFYATAGLPLRDLSLRRAFDHQTAVLLAEQRPDLIITCGYLHILT
                     APLLEAYPGRIFNVHDSDLPSYPGLHATRDAMIAGERSTRSVVHVVTAGVDAGPVLVR
                     SWPFPTHPLVGDARRWGAVDILRAYAYAQREWMMRSAWGPLMDAAIRCFADGAVPAGE
                     EAQPLARTLDPPEPAAMVAVARAAGAA"
     gene            complement(1314..2366)
                     /gene="hypE"
                     /locus_tag="AC16JC_06090"
     CDS             complement(1314..2366)
                     /gene="hypE"
                     /locus_tag="AC16JC_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015233565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HIR5164302.1"
                     /translation="MTATTTARGGDAVITLRHGAGGRAMRQLIGETLAAGYLQTPLPI
                     EGIGLASMDDGAALRVGDRWLVVTTDSHVVHPIFFPGGDIGRLAVAGTVNDLAMMGAT
                     TPLGLTCAIVMEEGFRRSDLERLQQSMQEACREAGVTILTGDTKVMGRGELDGIVINT
                     TGVALADAVVPDAGLRPGDRLILTGSIGDHGIALMVKRHGLAIEADLRSDVAPINGLI
                     AAALAARPGAVTAMKDPTRGGVASALHEMAEKSGVGIVIHEPAVPVSPVVRAAAEMLG
                     LDPFQIANEGKALLGVAPEAADAVLAALRAHPLGADAAIVGECNAERVGRVILDTGLG
                     RRLLVEVEGEPLPRIC"
     gene            complement(2363..>3372)
                     /gene="hypD"
                     /locus_tag="AC16JC_06095"
     CDS             complement(2363..>3372)
                     /gene="hypD"
                     /locus_tag="AC16JC_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HIR5164303.1"
                     /translation="MHVCGSHEQSIAKFGLRAVLPRDLNVIMGPGCPVCVTDVPEVDE
                     AIVLAQDGVRIATYGDMLKVPGSVHSLADAQARGARVDVVYSAAQAAELARDTTEDVV
                     FFASGFETTAVATAAVVLADPPPNFSVLSAHKYIPPVMEIVAEMPDTRVEGFLAAGHA
                     ATITGSRVFERFVERHKIPVVVAGFEPLDILGGLVKLVELVRDRTPRVENMFPRCVTP
                     EGNKVAQEALWRVFRTVGGNWRGIAHVPNGNLRLRDEFAAVDARKKYRIDVRRLWTRA
                     PSVLTQRCICGTIMAGTASPHDCPLFGKECVPDSPVGACMVSSEGTCKIWYQYGGLPD
                     L"
BASE COUNT          496 a         1186 c         1200 g          490 t
ORIGIN      
        1 tggccgagag ccgcgggttc aggagcacga gctccatggc cttgcgcagc cctacgaggc
       61 gcggcaggaa gaaggtcgaa ctctccgcac ccgtcagccc cgtcttgtga tacgcccact
      121 cgaacgaggc gcgctccgac gccatcacga ggtcgcacgc catggcgatg ccgaagccgc
      181 cggccgcggc cacgccgtcc acggcggcga cgaagggctt tggcgcccgc ttgatctccg
      241 agatcgtgct gtggatgtat tcgaggatct gcttgaagct ctcgccgtag ctcttccggg
      301 gaccggtccc ctcgggatgg aggtagccgg tgtcggtctc gtcgccgcgc gcgcggacgt
      361 acttgagatc ggcgccgctc gagaacacgc cctccacccc gcggagcacc acgcaccgga
      421 tggtgtcgtc gcgggcgagc tggatccccg ccttgcggaa atcctgcgcc atcacgacgt
      481 ccatcgcgtt gaagcgctcc ggtcggtcca tggtgatgat gccgaccgct tcctcacgcg
      541 tcgtgtggat gtgctccacc ggggttgcct gggcgtcggt catcacgcgg ctcctgccgc
      601 ccgggcgacg gcgaccatcg cggcaggctc tggagggtcg agggtgcggg ccaggggctg
      661 ggcttcctct ccggccggca ctgcgccgtc ggcgaaacag cggatcgctg cgtccatcag
      721 cgggccccag gccgagcgca tcatccactc gcgctgcgcg taggcgtacg ctcgcaggat
      781 gtccacggcg ccccagcggc gcgcgtctcc caccaggggg tgcgtcggga acggccagga
      841 gcgcaccagc accggcccgg cgtcgacgcc cgcggtcacg acgtggacga cggagcgcgt
      901 gctgcgctca ccggcgatca tcgcgtcgcg cgttgcgtgg aggccgggat aggagggcag
      961 atcgctgtcg tggacattga aaatgcggcc gggatacgcc tcgagcagcg gagcggtgag
     1021 gatgtgcagg tagccgcagg tgatgatgag gtccgggcgc tgctccgcga gcagcactgc
     1081 cgtctggtgg tcgaatgcgc gccgcaggga gagatcccgc agcgggagcc ccgccgtagc
     1141 gtagaacgcc cggatgtccc ggaccgccgc cggaacccct ccttccgtga cctgcacgag
     1201 gtcgcggtta gcgggatcgg tggcgatgaa cgccacgagc tgccgccgct ccagcaggta
     1261 atcgagcccc ggagcccggg tggtcgacag cacggcgacg cgcggcgagc ggctcagcat
     1321 atgcggggaa ggggttcgcc ttcgacctcg acgagcagcc gacgccccag gccggtgtcg
     1381 aggatgacgc gtcccacccg ctcggcgttg cactcaccca cgatcgcggc gtccgcaccg
     1441 agcgggtggg cccggagcgc cgccagcacc gcgtcggccg cctcgggcgc caccccgagc
     1501 agcgccttcc cctcgttcgc gatctggaag gggtcgagac cgagcatctc ggccgcggcc
     1561 cgcaccaccg gcgagacggg tacggcgggc tcgtggatca cgatgccgac cccgctcttc
     1621 tcggccatct cgtgcagcgc gctcgccacg ccgccgcggg tcgggtcctt catggcggtg
     1681 acggcgccgg ggcgcgcggc gagggcggcc gcgatgagac cgttgatcgg ggccacatcg
     1741 gagcgcagat ccgcctcgat cgccaagccg tgccgcttga ccatcagggc gatgccgtga
     1801 tcgccgatgg agccggtgag gatcaggcgg tcgccgggcc gcagtcccgc gtcggggacg
     1861 accgcatccg cgagcgccac gccggtcgtg ttgatcacga tcccgtccag ctcgccgcgt
     1921 cccatgacct tggtgtcgcc cgtgagaatg gtgaccccag cttcgcggca ggcctcctgc
     1981 atcgactgct gcagccgctc caggtcgctg cgccggaacc cttcctccat gacgatcgcg
     2041 caggtgagtc cgagcggcgt ggtggccccc atcatggcca ggtcgttgac ggtgccggcg
     2101 accgcgagcc gcccgatatc gccgcccggg aagaagatgg gatggaccac gtgtgaatcg
     2161 gtggtgacca ccagccagcg gtcgcccacg cgcaacgcgg cgccgtcgtc catcgacgcg
     2221 aggccgatcc cctcgatcgg caggggcgtc tgcaggtagc cggccgcgag cgtttcgccg
     2281 atcaactgcc gcatcgcgcg gccgccggcg ccgtggcgca gggtgatcac ggcgtcgccc
     2341 ccgcgggccg tcgtggtcgc tgtcataggt cgggaagtcc tccgtactgg taccagatct
     2401 tgcaggtgcc ttcgctcgac accatgcagg ccccgaccgg tgagtcgggc acgcactcct
     2461 tgccgaacaa cgggcagtcg tgcggcgagg cggtcccggc catgatggtg ccgcagatgc
     2521 agcgttgcgt cagcaccgag ggggcgcggg tccacaggcg gcgcacgtcg atgcggtact
     2581 tcttgcgggc atccactgcg gcgaactcgt cgcgcagccg caggttgccg ttggggacgt
     2641 gggcgatacc acgccagttg ccgcccaccg tccggaagac gcgccacagg gcctcctgag
     2701 cgaccttgtt gccctccggg gtcacgcagc gcgggaacat attctcgacc cgcggcgtgc
     2761 ggtcgcgcac cagctcgacc agcttcacga ggccgcccag gatgtcgagc ggctcgaacc
     2821 cggcgaccac caccgggatc ttgtgccgct ccacgaagcg ctcgaacacg cgcgagccgg
     2881 tgatcgtggc cgcatggccc gcggcgagaa acccctcgac gcgggtgtcc ggcatctcgg
     2941 ccacgatctc catgaccggc gggatgtact tgtgcgccga gagcacggag aagttggggg
     3001 gtggatcggc gagcaccacg gcggcggtgg cgacggcggt ggtctcgaac ccgctcgcga
     3061 agaagaccac atcctcggtg gtgtcgcggg ccagctcggc cgcctgcgcc gcgctgtaga
     3121 ccacgtccac ccgggcgccg cgggcctgcg cgtcggccag tgagtgtacg ctccccggca
     3181 ccttcagcat gtcgccgtag gtggcgatgc gcacgccgtc ctgggcgagc acgatcgcct
     3241 cgtccacctc gggaacgtcg gtcacgcaga cgggacaccc cggtcccatg atgacgttca
     3301 ggtcgcgcgg caggacggcg cgcagcccga actttgcgat cgactgctcg tggctgccgc
     3361 agacgtgcat ca
//
LOCUS       DBNGZW010000256         5473 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1522857, whole genome shotgun sequence.
ACCESSION   DBNGZW010000256 DBNGZW010000000
VERSION     DBNGZW010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5473)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1522857"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5473)
                     /locus_tag="AC16JC_06100"
     CDS             complement(<1..>5473)
                     /locus_tag="AC16JC_06100"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 94 protein"
                     /protein_id="HIR5164304.1"
                     /translation="RLVLTQRRLLEWETAASAAARAAGLSLRAGALLFLVEMAASPFI
                     ALILLVLIVTVRPSGLPVAGPLLAVWVAAPLVAYWLSRPVSLERHDLAPDDRRLLRLV
                     ARRTWRYFETFMGAQDHDLPADNFQETLVPVPTVAHRTSPTNIGMGLLAALAAHDLGF
                     IGTGELVQRLEATLSTMERLDRFEGHLLNWYDTTTLAPLAPRYVSTVDSGNLAAALLA
                     LAEGLQRLAGEPQAAARICGALADTAELARQSLKRLADTLAAPRNGTSGLAAAVSSIS
                     GALEDAEDAEGKLALAAALRPALSEAIARFEGETEPSPDRSEAIYWSQALATGLMASP
                     QDPVGFATRLETLARRAVAFADGMSFDFLYDWQRQIFATGYRLEDAQGSGRLDPSFYD
                     LLASEARLASFVAIAKGDVPDDHWFHLGRLLTSVDGAPTLLSWSASLFEYLMPLLVMK
                     SYPGTLLDQSCRMAVRRQIEYGKQQGVPWGISESAFNVVDHHGTYQYKAFGVPGLGLK
                     RGLGDELVVAPYATALAAMVDPERAAHNFRRLAREGLEGPYGFYEAIDYTHRKADDGE
                     DASEPRPHSSRGTVVRAFMAHHQGMSLVALANALLDSPMVQRLHADPRVKATALLLQE
                     RVPRHTPISRPRPAEETRVAAPAAAEAVRRFRSPHTRYPHAQFLSNGAYTAVVTNAGG
                     GASFCRGKVVTRYREDSACDPGSQFLYLRDVRSGSVWSAAYHPTDREPEEYLVTFHAE
                     RAVFRRRQGDIATQLDIAVSTEDDVEVRRLTVTNRSDRPRELEVTSYAEIVLASAAAD
                     LAHPAFSNLFVETEYLPESAALVCARRPRAPDDAGVWAVHVLSVEGRMQGPVEWETDR
                     CRFLGRGRSPEDPTALDGRALSGTTGAVLDPIVSLRQRIRLAPGGFVRLSFATGMAVS
                     REGALAIAHKYHDPSAAARTFALAFTQAQSTLRHLGISSDDAHLFERLASRVLYADAS
                     LRAGPEVFGRNVLGQPGLWAHGISGDLPILLVRVASGAAFPLAQQVLQAQEYWRLKGL
                     SADVVIVNEHPAGYVDDVHVQLAALLDTGPWGAWKHRPGGVYLLRGDHMSEDERSLLD
                     SVARVILDGARGELSSQLDLPYPEPQWRRELPPAPSRPVLQPDTPEIEAPELTFANGT
                     GGFADGGREYVVVLDGDQETPLPWVNVMANPGFGTVVSASGSAHTWADNSRENRLTPF
                     ANDPVTDPTTEALFVRDEDGGDVWSPTPGPTRRTSGSGRFVIRHAAGVSRFTHASHGI
                     LQELAVFVDAQDPVKFSLLTLTNRTRRRRRLSVFAYNEWRLGPPQPGEHLHVMTERDG
                     ETGAVLATNPYNQDFAGRVAFAHVGETARSASGDRLSFLGRNGTLARPAALRQRELSG
                     QFGAGLDPCAALQVEIALGPGESRRLVLILGQGRDHAHARALVRRHGSVDAADSALQT
                     VKRFWADTLETVQVRTPDDSFDLLMNRWLLYQDLSCRLWARTGFYQPSGAFGFRDQLQ
                     DVMALSLARPDVFREHLLRAAGRQFLEGDVQHWWHEPSGRGLRTRCSDDLLWLPYAVA
                     HYVDTTGDQGILDERVPFLEAPLLAAAETEAYGQPQVSTTTASLFDHCVRAIDKGLTA
                     GAHGLPLIGSGDWNDGMNRVGFQGRGESVWLGWFLHTVLRQFIPLCEQRDPGRATRYA
                     GETSRLAHLLERSWDGEWYRRGYYDDGAPLGSAQNDECKIDSIAQSWAVLSGAAPRRR
                     AEQAMDAVRTQLVRRGARVVLVLAPPFDRSAQDPGYIKGYPPGVRENGGQYTHAAVWT
                     VMAVARLGNGDEAVELFHLLNPINHTRSAADVERYKTEPY"
BASE COUNT          835 a         1882 c         1950 g          806 t
ORIGIN      
        1 cgtacggctc ggtcttgtac cgttcgacgt cggcggcgct ccgcgtatgg ttgatcgggt
       61 tcagcaggtg aaacagctct acggcctcgt cgccgtttcc gagccgggcg acggccatca
      121 cggtccacac cgcggcgtgg gtgtattgcc ccccgttctc ccgcacccct ggcgggtatc
      181 ctttgatgta accggggtcc tgcgccgacc gatcgaacgg gggcgccagt acgagaacga
      241 cccgtgcgcc gcgccgcacc agctgcgtgc ggacggcgtc catcgcctgc tcggcgcgcc
      301 ttctgggcgc ggcgccggac aggacagccc aggattgggc gatcgagtcg atcttgcact
      361 cgtcgttctg cgccgagccg agcggggctc cgtcgtcgta atatccccgc cggtaccact
      421 ccccgtccca ggaccgctcg agcaggtgcg ccagccggct ggtctcgccg gcgtaccgcg
      481 tcgctcgtcc cgggtcccgc tgctcgcaca gcgggatgaa ctgccgcaac accgtgtgca
      541 ggaaccaccc gagccagacg ctctcgcccc ggccctggaa cccgacccgg ttcatgccgt
      601 cgttccaatc gccgctgccg atcagcggaa gaccgtgcgc gccggcggtg aggcctttgt
      661 cgatcgcgcg cacacaatgg tcgaagagcg acgccgtagt ggtcgagacc tgaggttgtc
      721 catacgcctc cgtctcggcg gcggcgagga gcggcgcctc gaggaacgga acacgctcgt
      781 cgaggatgcc ctggtctccc gtcgtgtcca cgtagtgcgc caccgcgtag ggcagccaga
      841 gcagatcgtc cgagcagcgg gtgcgcaggc cgcggcccga cggctcgtgc caccagtgct
      901 gcacgtctcc ttcgaggaac tgccggccgg cggcgcggag gaggtgctcc cggaacacgt
      961 cgggccgagc cagcgagagg gccatgacgt cctgaagctg atcgcggaac ccgaaggcgc
     1021 cgctcggctg atagaagccg gtccgcgccc agagccggca gctcaagtcc tggtacagga
     1081 gccagcggtt catgagcaga tcgaacgaat cgtccggggt gcgcacctgg acggtctcga
     1141 gcgtgtcggc ccagaatcgc ttcaccgtct gtaatgcgct gtcagcggcg tccaccgagc
     1201 catggcgccg caccagcgca cgtgcgtgcg catgatcccg cccctgtccc aggatgagaa
     1261 cgaggcggcg actttccccc ggccccaggg cgatctcgac ctgcagggcc gcgcacggat
     1321 ccagtcccgc accgaactga cccgacaact cgcgctggcg gagcgcggcg ggccgcgcca
     1381 gcgtgccgtt ccgtcccagg aaggagagcc gatccccaga ggcggagcgc gccgtctcgc
     1441 cgacgtgcgc gaaggcgacc cggcccgcaa agtcctggtt gtaggggttc gtggccagga
     1501 cggcgcccgt ctccccgtcg cgctccgtca tcacgtggag gtgttcccct ggctgcggcg
     1561 gcccgaggcg ccattcgttg tatgcgaaga cgctcagtcg ccgccgccgc cgggtccggt
     1621 tggtgagggt gaggagcgaa aacttcacgg ggtcctgcgc gtccacaaag acggcgagct
     1681 cctggaggat cccgtgcgag gcatgcgtga agcgggaaac gccggctgcg tgccggatca
     1741 cgaaccggcc gctccccgat gtccgtcgcg tcggtccggg tgtcggggac cagacgtcac
     1801 ctccgtcctc gtccctcacg aacagcgcct cggtcgtcgg gtcggtcacg gggtcgttgg
     1861 cgaacggggt gaggcggttc tcgcggctgt tgtccgccca cgtatgagcc gagcccgatg
     1921 ccgagaccac cgtgccaaag ccggggttgg ccatgacgtt cacccacggg aggggggtct
     1981 cctggtcacc gtcgagcacg accacgtact cgcggccgcc gtcggcgaag ccacccgtcc
     2041 cgttggcgaa ggtgagctcg ggcgcttcga tctccggggt gtccggctgg agaaccgggc
     2101 gcgagggtgc cggtggcagc tcccgccgcc attgtggctc gggatacggc aagtccagct
     2161 ggctcgacag ctcgccgcgc gcgccgtcga ggatcacccg ggccacgctg tcgagcaggc
     2221 tccgctcatc ctcgctcatg tggtcgccgc gcagcaggta caccccaccg ggccggtgct
     2281 tccatgcccc ccagggtccg gtgtcgagca acgccgccag ctgcacgtgg acgtcgtcga
     2341 cgtagcccgc cggatgctcg ttcacgatca ccacgtccgc gctcagccct ttgaggcgcc
     2401 aatactcctg tgcctggagg acctgctgcg cgagggggaa ggcggcgcct gaggccacgc
     2461 gtaccaggag aatggggaga tcgcccgaga tcccgtgcgc ccacaacccc ggttgtccca
     2521 ggacgttgcg cccgaacacc tccggcccgg cgcgcagcga ggcgtcggcg tacagcacgc
     2581 gggaggcgag gcgctcgaag aggtgggcat cgtcgctcga gatgccaagg tgccgcagcg
     2641 tgctctgcgc ctgggtaaag gccagcgcga aggtacgcgc ggcggcactg ggatcgtgat
     2701 acttgtgggc gatggcgagc gcgccctccc ggctcaccgc catgcccgtc gcgaaggaga
     2761 ggcgcacgaa tccgccgggg gccaggcgaa tgcgctggcg caggctgacg atcggatcga
     2821 gcacggcccc ggtggtgccc gacaaggcac gtccgtcgag cgcggtcgga tcttcggggc
     2881 tgcggccccg gcccaggaac cgacagcggt cggtttccca ttccactggc ccctgcattc
     2941 gcccttccac gctcagtacg tgaaccgccc acacgccggc atcatcgggc gcgcgcggcc
     3001 gccgcgcaca gaccagcgcg gcgctctcgg gcaggtactc cgtctcgaca aagaggttac
     3061 tgaaggccgg gtgggccaga tccgcggcgg cagaggccag gacgatctcg gcgtagctcg
     3121 tgacctcgag ctcgcgcggc cggtcgctcc ggttggtcac ggtgaggcgc cggacttcga
     3181 cgtcgtcttc ggtggagacc gcgatgtcga gctgcgtcgc gatatcaccc tgacggcggc
     3241 ggaacacggc ccgctcggca tggaaggtga ccagatactc ttcgggctcc cggtcggtgg
     3301 gatgataggc tgcggaccag acggatccgc tgcgcacgtc ccgcaggtag aggaactggc
     3361 tccccgggtc gcaggccgag tcctcgcggt accgggtgac gaccttgccg cggcaaaaac
     3421 tggctccgcc tcccgcgttg gtgacgacgg cggtgtaggc gccgttcgac agaaactgcg
     3481 cgtgcgggta gcgcgtgtgg ggcgaacgga accgccgcac cgcctcggcg gcggccggcg
     3541 ccgccacccg cgtctcctcg gcgggccggg gccggctgat cggcgtgtgg cgcggcacgc
     3601 gctcctgcag cagcagcgcg gtggctttca ctcggggatc ggcgtggaga cgctgtacca
     3661 tcggggagtc gagcagggcg ttggccagcg ccaccagact catcccctga tgatgggcca
     3721 tgaaggcccg aacgacggtc ccgcgggagc tatggggtcg tggctcgctt gcgtcctcac
     3781 cgtcgtccgc cttgcggtgc gtgtagtcga tcgcttcata gaacccgtac gggccttcca
     3841 gcccttcccg ggcgaggcgc cggaagttgt gggcagcccg ctccgggtcg accatcgcgg
     3901 cgagcgcggt ggcgtagggg gccaccacca gctcgtcgcc caggcctcgc ttcagcccca
     3961 ggccgggaac gccgaacgcc ttgtactgat aggtcccgtg gtggtcgacc acattgaagg
     4021 ccgactccga gattccccag ggaaccccct gttgcttccc gtattcgatc tgccggcgca
     4081 cggccatgcg gcacgattgg tcgaggaggg tgcccgggta gctcttcatc acgaggagcg
     4141 gcatgaggta ttcgaacaga ctggcgctcc acgacagcag ggtgggcgcg ccgtccacgc
     4201 tggtcagcaa ccgtcccagg tggaaccagt gatcgtcggg gacatcgcct ttggcgatgg
     4261 cgacgaagct ggccaggcgg gcctcggacg ccaacagatc ataaaaggaa ggatcgagac
     4321 ggcctgagcc ttgcgcgtct tcgagacgat agccagtggc gaagatctgg cgctgccagt
     4381 cgtagaggaa gtcgaagctc atgccgtcgg cgaacgcaac ggcgcgccgc gccagcgttt
     4441 cgagccgcgt cgcgaacccg accgggtctt ggggcgacgc catcaacccc gtcgccagcg
     4501 cttgcgacca atagatcgcc tcggaccggt cgggggacgg ttctgtctcg ccctcgaacc
     4561 gtgcgatcgc ctcggagagc gccggacgca gtgcagcggc gagcgccagc ttcccctctg
     4621 cgtcctcggc atcctccaac gcgcccgaga tggacgagac ggcggcggcc aggccggacg
     4681 tgccgttccg gggggcggcg agcgtgtctg ccaggcgctt cagggactgc cgcgccagtt
     4741 ccgccgtgtc ggccagggcg ccgcagatcc gggctgccgc ctggggctcg ccggccagtc
     4801 gttgcaggcc ttccgccagg gccaggagcg cggcggcgag gtttccgctg tccacggtcg
     4861 agacataccg gggcgcgagc ggtgccagcg tcgtcgtgtc gtaccagttg agcagatgac
     4921 cctcgaaacg atcgagccgc tccatggtgg agagcgtcgc ctcgagccgc tgcaccagct
     4981 cgccggtgcc gatgaagccg aggtcgtgcg ccgccagcgc cgcgaggaga cccatcccga
     5041 tgttcgtggg tgacgtgcgg tgcgcgaccg tcgggaccgg taccagcgtc tcctggaagt
     5101 tgtcggcggg caggtcatgg tcctgggcgc ccatgaacgt ctcgaagtag cgccaggtcc
     5161 tgcgggcgac gagtcggagc aggcggcggt catccggagc aagatcgtga cgctcgagcg
     5221 acaccggccg gctgagccag tacgccacca gcggcgcggc cacccacacg gccagcagcg
     5281 ggcccgccac cgggaggccg ctcggccgga cggtgacgat gaggacgagc aggatcaggg
     5341 cgatgaaggg gctggccgcc atctccacga ggaagaggag cgcgccggcc cgcaggctga
     5401 gccccgctgc ccgggcagcg gccgacgccg cggtttccca ctcgaggagg cggcgctgcg
     5461 tcaggacgag gcg
//
LOCUS       DBNGZW010000257         1649 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_295136, whole genome shotgun sequence.
ACCESSION   DBNGZW010000257 DBNGZW010000000
VERSION     DBNGZW010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1649)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_295136"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="AC16JC_06105"
     CDS             complement(<1..557)
                     /locus_tag="AC16JC_06105"
                     /inference="COORDINATES: protein motif:HMM:NF012363.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HIR5164305.1"
                     /translation="MRSSWVQHSIAAAAVALSVSAGGPGVLLAAPKSPVVATDLGPVR
                     GVATGTMQEFRGIPYAAAPVGDLRWRPPQEHERWNGVRDATSFGPHCPQVATPYGTPS
                     TSEDCLFLNVFTSGQTNRGQPHLLPVMFWIHGGGLVVGESDDYDPSRLVAEDVIVVTI
                     NYRLGELGFLAHPAFAAESPSGAAG"
     gene            985..>1649
                     /locus_tag="AC16JC_06110"
     CDS             985..>1649
                     /locus_tag="AC16JC_06110"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HIR5164306.1"
                     /translation="MDVKAGDAAETDHAFAGAAHVVRLQTWVHRVTGVPMEPRTALAT
                     WDPASGRCTIRVQAGGLGRIQVGVAAALGVPESAVRATARDVGANFGTRNSCYPESVL
                     VAWAARRLGCPVKWCADRREAFLADYHGRDLGVEAELALAPDGRFLALRSSNTSNLGA
                     HAVHFGPLNKGMAIATTVYDVPVVSLRGRAVVTNTSPTTPYRSSGRPEVMFVIERLID
                     LAAR"
BASE COUNT          267 a          551 c          559 g          272 t
ORIGIN      
        1 ttacccgcgg cgccgctcgg tgactcggcc gcgaacgccg gatgggcgag gaagccgagc
       61 tcgccgagcc ggtagttgat ggtgacgacg atgacgtctt ccgccaccag cctgctcgga
      121 tcgtagtcgt cgctctcgcc gacgacgagt cctccgccgt ggatccagaa catcacggga
      181 agcaggtgag gctgaccccg gtttgtctgg cctgacgtaa acacgttcag gaacaggcaa
      241 tcctcgctcg tactcggtgt tccgtagggc gtagcgacct gagggcagtg agggccgaaa
      301 ctcgtcgcgt ctcgaacgcc gttccagcgc tcgtgctcct gcggcgggcg ccaccgaagg
      361 tctccgacgg gcgccgcggc gtacgggatt ccgcggaact cctgcattgt gccggtcgcg
      421 acacccctca ccgggcccaa atccgtggcc acgaccggcg atttgggtgc tgccagcagg
      481 acgcccggac cacccgcgga taccgacaat gctacagctg ctgctgcgat actgtgttgg
      541 acccagctcg accgcatatc ctcctcccgc cggagatata cgagggcgtg caggacacta
      601 tcccaggggg aatcgcgagt caagggcttc gtttcccgat gaagtatcta ctcaagcttg
      661 accgtctttg ctttgatggg cagccagacc gggctgtccg gatcaagggg gggccaatga
      721 tcaagctcag cgacgtgaag gttcggccgc agtatcggac gtgggcgcgg catcgcctga
      781 tcgtgttgga gtacgcgtgg gcacacgggc cagcggccgc agatccggtg ctcatcgccg
      841 aagctgtgcg gagtgccggc ggtcaatgat ccggtcgcgg aaaccccgcc cacgtctcgg
      901 gctccctacc ctaagcgtgg ggaagcagga acagcgccgg actcagatcc taggaactct
      961 ccagccctga cgtcgaacgt ctgcgtggac gtgaaggccg gcgacgcggc cgaaaccgat
     1021 cacgcgtttg caggcgccgc gcacgtggtc cggctccaga catgggttca tcgcgtcacc
     1081 ggcgtgccca tggagccgcg cacggcgttg gccacctggg atccggcgag cggtcgctgc
     1141 accatccgcg ttcaggccgg cggcctcgga cggatccagg tgggcgtcgc cgccgcgctc
     1201 ggtgtccccg agagcgcggt gcgcgcgacg gcgcgcgacg tcggcgcaaa ttttgggacg
     1261 cgcaactcct gctacccgga gtccgtgctg gtggcgtggg cggcgcggcg ccttggatgc
     1321 ccggtgaagt ggtgcgcgga ccggcgcgag gcgtttctcg ccgactatca cgggcgcgat
     1381 ctcggggtcg aggccgagct agcgctcgcc cccgacggac gattcctcgc gctgcgcagc
     1441 agcaacacga gcaatctcgg cgcgcacgcc gtgcatttcg gcccgctcaa caagggcatg
     1501 gcgatcgcca ccaccgtcta tgacgtgccc gtcgtgtcgt tgcgcggacg tgccgtggtc
     1561 accaacacgt cgcccacgac cccgtaccgc agctccggac gtcccgaggt catgttcgtg
     1621 atcgagcggt tgatcgacct cgccgcgcg
//
LOCUS       DBNGZW010000258         4101 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1379550, whole genome shotgun sequence.
ACCESSION   DBNGZW010000258 DBNGZW010000000
VERSION     DBNGZW010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1379550"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            79..381
                     /gene="rpsS"
                     /locus_tag="AC16JC_06115"
     CDS             79..381
                     /gene="rpsS"
                     /locus_tag="AC16JC_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013569719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HIR5164307.1"
                     /translation="MARSVKKGPYVEPSLLKKIQAMNDRNEKKVFKTWSRRSTITPDF
                     VGHTLAVHNGNKFVPVYVTENMVGHKLGEFSATRLFRGHTTKTAAEREAEAKEAQA"
     gene            385..723
                     /gene="rplV"
                     /locus_tag="AC16JC_06120"
                     /pseudo
     CDS             385..723
                     /gene="rplV"
                     /locus_tag="AC16JC_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003247572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            889..1548
                     /gene="rpsC"
                     /locus_tag="AC16JC_06125"
     CDS             889..1548
                     /gene="rpsC"
                     /locus_tag="AC16JC_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HIR5164308.1"
                     /translation="MGQKTHPYGFRLGVIKPWRSRYYARRNFPELLKEDDLIRRYLKT
                     RLSHAAIADVHIERRPGKVTVTVHTGRPGVVIGKRGAEVDKLRDELAQLTGKEVGINV
                     EEIKRPELDAQLVGDSVVHQLQQRISFRRAMKRAVQSAMRMGAQGIKIKCGGRLGGAE
                     IARVEGYHEGRVPLHTLRADVDYATSTAKTTYGTIGVKVWIFKGERTEELAGRTYSTG
                     V"
     gene            1551..1964
                     /gene="rplP"
                     /locus_tag="AC16JC_06130"
     CDS             1551..1964
                     /gene="rplP"
                     /locus_tag="AC16JC_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013290157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HIR5164309.1"
                     /translation="MLAPKRVKFRKRFKGNTNGLATRGNTVAFGHFGLMSLDPGWITN
                     RQIEAARVALTREMKRGGKVWIRLFPDKPITKKPAETRMGKGKGNPEGWVAVVKPGRV
                     MFEVEGIEESLARHALTLAAAKLPLRSKFVKREER"
     gene            1966..2178
                     /gene="rpmC"
                     /locus_tag="AC16JC_06135"
     CDS             1966..2178
                     /gene="rpmC"
                     /locus_tag="AC16JC_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HIR5164310.1"
                     /translation="MARQRPDDLRQLKTDELEQKLAVLREERFRLRFRAGTEAITNPL
                     QFRVMRRDIARIETILRERAVAGRQA"
     gene            2175..2423
                     /gene="rpsQ"
                     /locus_tag="AC16JC_06140"
     CDS             2175..2423
                     /gene="rpsQ"
                     /locus_tag="AC16JC_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016444130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HIR5164311.1"
                     /translation="MRTRRKTRTGVVTSDKRLKTVTVALVRRYAHGFYGKQVVRTKTV
                     AAHDEQGAKTGDTVRVEETRPLSKTKRWRVVEILERAK"
     gene            2439..2807
                     /gene="rplN"
                     /locus_tag="AC16JC_06145"
     CDS             2439..2807
                     /gene="rplN"
                     /locus_tag="AC16JC_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HIR5164312.1"
                     /translation="MVQQESILKIADNSGARRALVIRVLGGSKRRYAGLGDIVVVTIK
                     DAIPTGQIKKGEVAKAVIVRTAKETKRRDGSYIRFDENAAVLITDQGEPRATRIFGPV
                     GRELRDKKFMKIVSLAPEVI"
     gene            2809..3192
                     /gene="rplX"
                     /locus_tag="AC16JC_06150"
     CDS             2809..3192
                     /gene="rplX"
                     /locus_tag="AC16JC_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HIR5164313.1"
                     /translation="MKPLVYKKSRRVRGTEPVRHKMHFAKGDTVRVIRGDDKGKEGKV
                     LHVHPKMLRVTVEGVNIVKKHQRARTAEQESKIIERPAPIAASKVMLLDPKSGAPTRV
                     RRRRDKDGTVERIAVKSGQPIPRVR"
     gene            3195..3857
                     /gene="rplE"
                     /locus_tag="AC16JC_06155"
     CDS             3195..3857
                     /gene="rplE"
                     /locus_tag="AC16JC_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HIR5164314.1"
                     /translation="MAETKEKKTKPAKPAKGAPPAEAAEPQVELEPAPPARLRAHYEQ
                     RVRPKLQQQFGFSNPHQIPKLVKIVLNVGMGDAPKNPKSLETAAAELGAITGQRPVIT
                     RAKKAIANFKLRAGVPIGCAVTLRGPRMYEFLDRFISVTVPRMRDFRGLPTKSFDGRG
                     NYTLGIKEQMVFPEIDYDKVEQIHGMDITFVTNAGRDDTAMALLREMGMPFRGESPVA
                     VG"
     gene            3869..4054
                     /locus_tag="AC16JC_06160"
     CDS             3869..4054
                     /locus_tag="AC16JC_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR5164315.1"
                     /translation="MAKKCWIERVKRTPKFKVRAYQRCQRCGRSRATLRKFGLCRICF
                     RELALRGEIPGVRKASW"
BASE COUNT          807 a         1216 c         1557 g          521 t
ORIGIN      
        1 tttcggattg tggattgcgg atttgtcggc ggtgttcaat ccgcaatccg caatcgccaa
       61 tccgcaatgg agagtgaaat ggcacgcagc gtcaagaagg gtccgtacgt ggagccgtcg
      121 ctcctgaaga agatccaggc gatgaacgac cgcaacgaga agaaggtgtt caagacctgg
      181 tcgcggcgca gcacgatcac gcccgacttc gtgggccaca cgctggcggt gcacaacggc
      241 aacaagttcg tgccggtgta cgtgaccgag aacatggtgg ggcacaagct gggcgagttc
      301 tcggcgacgc ggctgttccg cgggcacacc acgaagacgg cggcggagcg cgaagccgaa
      361 gcgaaggagg cgcaggccta agccatggaa gcccgggcga ttcagcgcac ggtgcgccag
      421 tcggcgcgca agatgcgcct ggtgatcgat cagatccgtg ggcgcgcggt gccggaggcg
      481 tacgccatcc tgcggttttc gaagaagcac gcggcgcggc agatccacaa ggtgctcaag
      541 tcggcggtgg ctaatgcgga gcagcacgcg cagcggaaca acgtcgcgct ggacgtggac
      601 gggctccggg tgggctatgc ggtcgtgaac gaagggccga cgctcaagcg gttcacgatg
      661 ggcgcgatgg gtcgcggcat gccgatcctc aagcggacga gccacgtgga gattcacgtg
      721 agcgcgcccg atgtggtgac ggccccggcg ccgaaaccgg cggccaaacc gtcgggcaag
      781 tccaaggaga agccggcggc cgagaagccc gccgccaagg cgaaggccaa ggccgcgagc
      841 gcgaccaagg ccaaggccaa gcgcaagtcg aaggggagga gcaagtaagt gggccagaaa
      901 acacatccct acgggttccg gctgggggtc atcaagccgt ggcgctcgcg ctactacgcg
      961 cgccgcaatt tccccgagct cctgaaggaa gacgacctga tccgccgcta tctcaagacg
     1021 cggctcagcc acgcggcgat cgccgacgtg catatcgagc ggcgcccggg gaaggtgacg
     1081 gtcacggtgc acaccggccg gccgggcgtg gtgatcggca agcgcggggc ggaggtggac
     1141 aagctgcgcg atgagctggc gcagctcacc ggcaaggagg tggggatcaa cgtcgaggag
     1201 atcaagcgcc ccgagctgga cgcccagctg gtgggcgaca gcgtggtgca ccagctgcag
     1261 cagcgcatct cgttccgccg cgcgatgaag cgggcggtgc agagcgcgat gcggatgggg
     1321 gcgcagggga tcaagatcaa gtgcggcggg cgcctgggcg gcgcggagat cgcgcgcgtg
     1381 gagggctacc acgagggccg cgtgccgctg cacacgctgc gggccgacgt ggactacgcc
     1441 acgtcgaccg ccaagacgac gtacggcacg atcggcgtga aggtgtggat cttcaagggt
     1501 gagcggaccg aggagctggc gggccgcacc tactcgacgg gcgtctgacg atgctggccc
     1561 ccaagcgcgt caagttccgc aagcggttca agggcaacac caacgggctc gccacgcgcg
     1621 gcaataccgt cgcgttcggg cacttcgggc tgatgtcgct cgatccgggc tggatcacca
     1681 accgccagat cgaagcggcg cgcgtcgcgc tcacgcgcga gatgaagcgc ggcggcaagg
     1741 tgtggatcag gctgtttccc gacaagccga tcaccaagaa gccggccgag acccggatgg
     1801 ggaaggggaa gggcaacccg gaggggtggg tggcggtggt gaagccgggc cgggtgatgt
     1861 tcgaggtcga ggggatcgag gagtcgctgg cgcgccatgc gctcacgctg gccgcggcga
     1921 aactgcccct ccggtcgaag ttcgtgaagc gggaggagcg gtaaaatggc gcggcagcga
     1981 cccgacgacc tgcggcagct gaagaccgac gagctggagc agaagctcgc cgtgctgcgc
     2041 gaggagcggt tccgcctgcg gttccgggcg gggacggagg cgatcacgaa cccgctgcag
     2101 ttccgggtga tgcggcggga catcgcgcgc atcgagacca ttctgcgcga gcgcgcggtg
     2161 gcagggaggc aggcatgagg acccggcgga agacgcggac gggcgtggtg acgagcgaca
     2221 agcggctgaa gaccgtgacg gtggcgctgg tgcgccgcta cgcccacggc ttctacggca
     2281 agcaggtggt ccgcaccaag acggtggcgg cccacgacga gcagggggcc aagaccggag
     2341 acaccgtccg ggtggaggag acgcgcccgc tctcgaagac gaagcgctgg cgggtggtcg
     2401 agattctcga gcgggcgaag taagggccgg aggggaccgt ggtacagcag gaatcgatac
     2461 tcaagatcgc ggacaattcg ggcgcccgga gggcgctcgt catccgcgtg ctcggcgggt
     2521 ccaagcggcg ctacgcggga ctcggcgaca tcgtggtcgt cacgatcaag gacgccattc
     2581 ccacggggca gatcaagaag ggggaagtgg cgaaggcggt gatcgtgcgg acggccaagg
     2641 agaccaagcg ccgggacggc tcctacatcc ggttcgacga gaacgccgcc gtgctgatca
     2701 ccgatcaggg cgagccgcgg gcgacccgca tcttcggccc ggtcggccgg gagctgcgcg
     2761 acaagaagtt catgaagatc gtgtcgctgg cgccggaggt gatctgacat gaagccgctg
     2821 gtctacaaga aatcgcggcg cgtgcggggc accgagccgg tgcgccacaa gatgcacttc
     2881 gccaagggcg acaccgtccg ggtgatccgg ggcgacgaca aaggcaagga aggcaaggtc
     2941 ctgcacgtgc accccaagat gctgcgggtg acggtggagg gcgtgaacat cgtgaagaag
     3001 caccagcggg cgcggaccgc cgagcaggag agcaagatca tcgagcggcc cgccccgatc
     3061 gcggcatcga aggtgatgct gctcgatccc aagtcgggcg cgcccacgcg ggtgcgccgg
     3121 cggcgggaca aggacggcac ggtcgagcgg attgcggtca aatcgggaca gccgatcccg
     3181 cgggtgaggt gagcatggcg gagaccaagg aaaagaagac caaaccggca aagccggcga
     3241 agggcgcgcc gcccgcggag gcggccgagc cccaggtgga gctcgagccg gcgccgccgg
     3301 cgcggctgcg ggcgcactac gagcagcgcg tgcggccgaa gctgcagcag cagttcggat
     3361 tctcgaaccc gcaccagatc ccgaagctcg tgaagatcgt gctgaacgtc gggatgggcg
     3421 atgcgccgaa gaacccgaag tcgctggaga cggcggcggc ggagctgggc gcgatcaccg
     3481 ggcagcggcc cgtgatcacg cgcgccaaga aggcgatcgc gaacttcaaa ctgcgggcgg
     3541 gcgtgccgat cggctgcgcc gtgacgctgc ggggtccgag gatgtacgag ttcctcgacc
     3601 ggttcatctc ggtgaccgtg ccgcggatgc gcgacttccg ggggctgccg accaagagct
     3661 tcgacggccg gggcaattac acgctgggca tcaaggagca gatggtcttc cccgagatcg
     3721 actacgacaa ggtcgagcag atccacggga tggacatcac gttcgtcacg aacgccggcc
     3781 gcgacgacac ggccatggcg ctgttgcgcg agatggggat gccgttccgc ggcgaatccc
     3841 cggtcgcggt cggctaggag aggacggcat ggcgaaaaag tgttggatcg agcgagtcaa
     3901 gcgcaccccg aagttcaagg tgcgggcgta tcagcgctgt cagcgctgcg gccgcagccg
     3961 ggccacgctc cgcaagttcg ggctgtgtcg catctgtttc cgtgagctgg cgctgcgcgg
     4021 ggaaattccc ggcgtccgca aagccagctg gtaggcggga ggcgcaatga gcatgactga
     4081 tcccgtcgcc gatatgctca c
//
LOCUS       DBNGZW010000259         3352 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_909150, whole genome shotgun sequence.
ACCESSION   DBNGZW010000259 DBNGZW010000000
VERSION     DBNGZW010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_909150"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..303)
                     /locus_tag="AC16JC_06165"
     CDS             complement(<1..303)
                     /locus_tag="AC16JC_06165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164316.1"
                     /translation="MRILRSCAVLLFCAAPVVAQGPRPLNWDSIAAEAVRNLSAYLAI
                     NTSNPPGNELAAARYLQQLLARDGIEAQILDTAELGPGRANLYARLRSSGTKKAIAL"
     gene            complement(390..1589)
                     /locus_tag="AC16JC_06170"
     CDS             complement(390..1589)
                     /locus_tag="AC16JC_06170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5164317.1"
                     /translation="MPAALDRLSRRLLLDRLARLSSGRLVVEDGADSLPFGPGPEPAA
                     RLIVHDPRFYRAVVLGGHLGAVDAYLDGWWDTDDLTALVRVMVQNHDVLDSLETGWAR
                     LASPVRRAWHAWRRNSRGGSRRNIAAHYDLGNDFFAEFLDDTLTYSCGVFERPDASLR
                     EASLAKYERLCRKLELGPADHVLEIGSGWGGFALHAAGTRGCRVTTTTISREQHRLAV
                     ERVAAAGLAQRVTVLLQDYRDLTGRYDKLVSIEMIEAVGHAFYRTFFRKCAELLAPGG
                     RMALQAITIDPRFYHRARREVDFIKRYIFPGSNIPSVPVLREAAERKAGLALIHLEDI
                     TPHYAETLKRWRENFLARWDRLRALGFSEQFRRLWEFYFCYCEGGFRERFLGDVQMVF
                     VREPTPA"
     gene            complement(1592..2476)
                     /locus_tag="AC16JC_06175"
     CDS             complement(1592..2476)
                     /locus_tag="AC16JC_06175"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015446502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006636 - unsaturated fatty acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HIR5164318.1"
                     /translation="MTTLRAVFTKLRLWFDTAATPAGGNPAEHRVDWVRVLPFVAMHL
                     ACLAVIWVGASWAAVGVAVGLYTTRMFFVTAFYHRYFSHRTFETSRPAQFAFAVLGAT
                     CVQRGPLWWAAHHRKHHRHADTPDDVHSPRLHGFWWSHMGWITSPENFPTDLQQVPDL
                     AKYPELRFLDRFDVLVPLLLALVLFLIGGAQWLVWGFFISTVVLFHCSCFINSLAHQL
                     GRRRYPTPDDSKNSLILSLITFGEGWHNNHHWYPGAARQGFFWWEVDLTYYGLWLLAR
                     IRIIRNLHPVPARVLERA"
     gene            2603..>3352
                     /locus_tag="AC16JC_06180"
     CDS             2603..>3352
                     /locus_tag="AC16JC_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164319.1"
                     /translation="MSRAPLAAAFTIALAGALPAGLLAQADSGQASPSRGRPGYRALN
                     LGVHDVGLSIGNSRRWTGLRINFQDNAVERVDGINLTVWNAKHNETMELNGVALGLAG
                     PAGGTFNGVTVGVIGAVADRGFHGVTLAGLGAVSDGDTRGITVAGLGVVSNGDMTGIN
                     VSGLGTVANRSMRGLNLAGLGVVADGDLGGISVSGLGTVANGVLGGVSVGGLAVVGNR
                     GVWGIATGGLAVVSNEAVDGVSAAGLAVVGNG"
BASE COUNT          538 a         1094 c         1229 g          491 t
ORIGIN      
        1 gagcgcgatc gccttcttcg tgccgctgga gcgcagccgg gcgtagagat tggcccggcc
       61 agggcccagc tcggcggtat cgaggatctg ggcttcgatg ccgtcgcgcg cgagcagctg
      121 ctgcagatac cgggcggcgg cgagctcgtt gcccggcgga ttggacgtgt tgatcgccag
      181 ataggccgag aggttgcgga ccgcctcggc ggcgatggag tcccagttga gcgggcgcgg
      241 cccctgggcg acgacggggg cggcgcagaa cagcagcacg gcgcaggagc gcaggattcg
      301 catgatgacg gtgtggtgag gggtggcgtg aaactgcggc cgagggcgcg gacccgcaag
      361 ctcggccgca cccggcaggc gagccggaat caggcgggcg tcggctcccg cacgaacacc
      421 atctgcacgt cgcccaggaa gcgctcgcgg aatccgcctt cacaatagca gaagtagaac
      481 tcccacaggc gacggaactg ctcggagaag ccgagcgcgc gcagccggtc ccagcgggcg
      541 aggaagttct cgcgccagcg cttcagggtt tcggcgtagt gcggcgtgat gtcttcgagg
      601 tggatcagcg cgaggcccgc cttgcgctcg gcggcttcac gcaacaccgg gacggagggg
      661 atgttcgagc cggggaaaat gtagcgcttg atgaaatcca cttcccggcg cgcgcgatga
      721 tagaagcgcg gatcgatggt gatcgcctgc agcgccatgc ggccgcccgg cgcgagcagc
      781 tcggcgcact tgcggaagaa cgtgcggtag aacgcatggc ccaccgcctc gatcatctcg
      841 atcgaaacga gtttgtcgta tcggccggtc aggtcgcgat agtcctgcag cagcaccgtc
      901 acccgttgtg cgagaccggc cgccgccacg cgctcgacgg ccaggcggtg ctgctcccgg
      961 gagatcgtgg tggtggtgac gcggcagccg cgcgttccgg cggcgtgcag cgcgaaaccg
     1021 ccccatccgc tgccgatctc cagcacgtgg tccgcgggcc cgagctcgag cttgcggcac
     1081 agccgctcgt acttggccag agacgcctcg cgcaggctgg cgtcgggccg ctcgaacacg
     1141 ccgcacgagt acgtcagcgt gtcgtcgagg aattcggcga agaagtcgtt cccaaggtcg
     1201 tagtgcgcgg cgatgttgcg ccggctgccg ccgcgcgaat tgcgccgcca ggcatgccat
     1261 gcccgtcgca ccggcgaggc gagccgggcc caccccgtct cgagactgtc cagcacatcg
     1321 tggttctgca ccatcacccg caccagggcg gtcagatcgt cggtgtccca ccagccgtcc
     1381 aggtacgcgt cgacggctcc caggtgccct cccaggacga ctgcccggta aaagcgtgga
     1441 tcgtggacga tgaggcgggc cgccggctcg ggtccgggac cgaacggcag ggagtcggcg
     1501 ccatcctcca ccacgagacg gccggaggac agccgcgcca gccggtcgag cagcagcctg
     1561 cgggacagtc ggtcgagggc ggccggcacg ctcaggcgcg ctccaggacc ctggccggca
     1621 ccgggtggag gttccggatg atcctgatgc gggcgagcag ccacaacccg tagtaggtca
     1681 ggtccacctc ccaccagaag aatccctgtc gcgcggcgcc gggataccag tggtggttgt
     1741 tgtgccaccc ctcgccgaag gtgatgagcg agaggatcag gctgttcttg ctgtcgtcgg
     1801 gagtcgggta gcggcggcgc cccagctggt gagccagcga attgatgaag cagctgcagt
     1861 ggaacaacac caccgtactg atgaagaacc cccacaccag ccattgcgca ccgccgatca
     1921 ggaacaggac cagggcgagc agcagcggga cgaggacgtc gaagcggtcg aggaagcgga
     1981 gctccgggta cttcgccaga tccggtacct gctgcaggtc ggtggggaag ttctctggcg
     2041 aggtgatcca ccccatgtgg ctccaccaga agccatgcag gcggggactg tgcacgtcgt
     2101 ccggcgtgtc cgcatgccgg tggtgcttac ggtggtgcgc cgcccaccag agcggccccc
     2161 gctgcacgca cgtcgcgccc agcaccgcga aggcgaattg ggccgggcgt gaggtctcga
     2221 aggtccggtg ggagaagtac cgatggtaga atgccgtcac gaagaacatg cgcgtcgtgt
     2281 agagtccgac cgcgacgccc accgcggccc agctcgcccc cacccagatc accgccaggc
     2341 aggcgaggtg catggcgacg aacggcagca cacgcaccca gtccacccga tgctcggccg
     2401 gattccctcc ggccggcgtc gccgcggtgt cgaaccacag ccgcagcttg gtgaataccg
     2461 ctcgtagtgt ggtcatcgtg cgcccatgaa acgtcgtcgt ccgcgctctg gcttgcaaga
     2521 tgcacgccgt ccccccatgt atggaacccg agccgccgcg gtcccgtact ggccgcatcc
     2581 ttccctctgc gcgaggacgc cgatgagccg ggccccgctt gctgccgcgt ttaccatcgc
     2641 gctggccggt gcgctgccgg ccggcctcct cgcgcaggcc gactccggcc aggcctcccc
     2701 gtcacgtggc cgcccggggt atcgcgcgct gaatctcggc gtgcacgacg tggggttgag
     2761 catcgggaac tcgcgtcgct ggaccggtct gcggatcaac ttccaggaca acgccgtcga
     2821 acgggtggac gggatcaacc tcacggtctg gaacgcgaag cacaatgaaa cgatggagtt
     2881 gaacggggtg gccctggggc tcgcgggtcc cgccggtggg accttcaacg gcgtgaccgt
     2941 cggggtgatc ggggccgtgg cggaccgggg gttccatggg gtgacgctcg ccggcctggg
     3001 cgcggtctcc gacggcgaca cgcgcggcat cacggtcgcc gggttgggcg tggtctcgaa
     3061 cggcgacatg acggggatca acgtgtcggg cctcgggacc gtggctaacc gctcgatgcg
     3121 cggtctgaac ctggcgggcc tgggtgtcgt cgcggacggc gacctgggcg ggatcagcgt
     3181 ctcgggtctc ggcaccgtcg ccaacggtgt gctgggtggg gtgagcgtgg gcggcctcgc
     3241 cgtcgtgggg aaccgcggcg tgtgggggat cgccaccggc ggtctcgcgg tcgtgtccaa
     3301 cgaggccgtg gacggcgtga gtgccgcggg cctggccgtg gtggggaacg gg
//
LOCUS       DBNGZW010000260         8776 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_23290, whole genome shotgun sequence.
ACCESSION   DBNGZW010000260 DBNGZW010000000
VERSION     DBNGZW010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8776)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8776)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8776
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_23290"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="AC16JC_06185"
     CDS             <1..288
                     /locus_tag="AC16JC_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007220837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide synthetase C
                     domain-containing protein"
                     /protein_id="HIR5164320.1"
                     /translation="FETSSKDAVIIFKKPTRDGVHIEDVKTVDNEWLVTGTSGVVLIV
                     VGLGPTMKAAQKQVYNRIENIMIPNMYYRKDIGDRWFEDFDRLHTWGFLRD"
     gene            302..1579
                     /locus_tag="AC16JC_06190"
     CDS             302..1579
                     /locus_tag="AC16JC_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5164321.1"
                     /translation="MSGDALEHATLRRVSRRLIPFLFVVFIANYLDRVNVSFAALQMN
                     RDLGLSGAAYGLGAGIFFLGYVLFPVPSSLLLARVGARRWIAAITTAWGGIASAMMFV
                     RGPATFYLLRFLLGVVEAGFFPGVILYFTYWFPAAHRARAVARFMTAIPVAQIVGGPL
                     SGWLLGFHGRAGLAGWQWLFLLEGLPSLALGYLVLTYLTDRPEDATWLAPEARAWLTG
                     RLRAEHPEDAAPARPWRRLLATPALWHLSGVWILMVLVGYGQLLWLPQIIKGASGLSN
                     LAVGFLSAAPPIAAAVAMVWIAAHSDHTGERRRHVALPCLLTAAGFAATAAAPRAPLL
                     ATAALTLVAIGIAGSFGPFWGLATTSLSGPASAGGIALVSSIGNLGGFAGPYVVGLLK
                     DATGSFAAVLVVFSGLAAAAGLLALRFRPAGRT"
     gene            1617..2648
                     /locus_tag="AC16JC_06195"
     CDS             1617..2648
                     /locus_tag="AC16JC_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HIR5164322.1"
                     /translation="MISSPHESAVIAGAGPIGLACAISARRRGLDPLIIDAGALAESI
                     VRYPVGMTFFTTPERLEIGGHPLVCAGAKATREEALKYYRGVARAEGLRVRPYARLVG
                     AEHRDGCIACDIETRLGGESIGCDRLVLATGYFDHPNALDVPGEALPHVHHYFDEAHR
                     SFNQDVVVIGGKNSAVEAALQLFRAGARTTLVYRGAELSRSVKYWLAPDLLNRIKAGE
                     IAARFGAEVVEITALHVRIRRADGTVEDLPADRVYALTGFHPDFDLCTRIGISFDPAS
                     GRPALDPATLETSVPGVHLAGSVTAGRKISEIFIENGRFDGDKIFGDAAARAAAQSRY
                     AGIDRPAGE"
     gene            2752..4116
                     /locus_tag="AC16JC_06200"
     CDS             2752..4116
                     /locus_tag="AC16JC_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014437891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR5164323.1"
                     /translation="MMSATTRPTRRPTMPPCAHAPRPYTGPSRAEVLATRKRYANPAI
                     FTIYREPLMLVEGHRQYLFDETGRRYLDLFAGIVTVSCGHAHPKVTERVQQQVATLTH
                     ATTIYLHPHIGEFARRLAATLPSGLDVTYFVNSGSEANDLAVLLARLATGHNDVIAVR
                     NGYHGGSPAAMALTSHHTWKFPGQLNTGVHHTVCPDPYRSPFTGTPEQIATRSVEEMR
                     DLIRFSTPGKIAAFIAEPIQGVGGVTHGAPNYLGEAYALTREHGGLCIADEVQTGFGR
                     TGDHYWGFENWGVVPDIVTMAKGIGNGFPLAAVTTRREIAERLTQRIHFNTYGGNPVS
                     MAAGLAVLEVIEEEGLQRNAGEVGGRLKAGLRELAARHPLIGDVRGMGLMLGVELVKD
                     RGTKEPAREAAAAVFERLREQGILVGKGGLFGNVLRIKPPLCLTAADVDFALDVLDQV
                     LAEV"
     gene            complement(4113..5513)
                     /locus_tag="AC16JC_06205"
     CDS             complement(4113..5513)
                     /locus_tag="AC16JC_06205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5164324.1"
                     /translation="MTFLGRGPAIEQACYWLARRPERTVTALPASRAADVAIVGAGLA
                     GLWTALFLKRLDSACDVVVLEQGLAGHGASGRNAGMLLETIDHSHGLAIRHFGEVEAR
                     RLARLGEANLVELTAFLRERGIDCDYEPTGRLVVALTPGQVVACRHMVETAEGLGVES
                     YQLLDKEAVRRAVNSPLYLGGVRVSAGGVLDPVKLVDGLLRDAERMGVTVYERCHVMG
                     IESLGDGVRLRSTRGRVDARRVVLTTGAYTHRLVPQVARRMIPLYGYMLASEPLTAAQ
                     RDVIGWRERQGVTDGRRFFNYYRLTRDDRILWGTSEPVYFSRDGVAPDHDHSPAQYEI
                     LRASFRRHFPALAGLEWAYGWGGPIDVTARLTPFFGQALDGRLLYGLGFSGLGLGPTR
                     IAGRVLAHLALERPSDLLDLPLVRRPPALFPPGPLRPWAVRSVKRALRRVDAGAEPGL
                     LLRLLDRLGIGGGMSS"
     gene            complement(5510..6325)
                     /locus_tag="AC16JC_06210"
     CDS             complement(5510..6325)
                     /locus_tag="AC16JC_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014816231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5164325.1"
                     /translation="MSAGSRRRGWGGLLDATAALVLLYLFLPIFVIVLYSFNRPEGRF
                     NFVWKSFSLDAWRHPFAYPQLVDAMLLSLKVAAVATVAATIFGTMIALALCRYRFRGS
                     GAVGLLLVLPLTTPEIVLGSSLLSLFLAVNARMGFATIVIAHIMFSVSYVALTVRARL
                     RGFDWTLEEAALDLGATPWRAFTRVTLPLIAPGIVAAAMLCFALSLDDFVITLFNSGA
                     AVTYPLYVYGARRAAFPPQINVLATSILVLSLGLLGATLLWQQRRARAAVRQP"
     gene            complement(6322..7182)
                     /locus_tag="AC16JC_06215"
     CDS             complement(6322..7182)
                     /locus_tag="AC16JC_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009714699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5164326.1"
                     /translation="MTAPAGAAAPAPGRRRLAPYVLLAPGVLWLVVFFLVPILALART
                     SLGAGDAPWWDGYARAFTGYGSFLIRSFRFAFVATLIALLLGYPLAYVMAFRSGAGRL
                     RHLLLGLVILPFFTAFLVRTFAWKTMLNDGGPVVGLLGWLHLLPAEGRLLNTSWAVIG
                     GLTYNFLPFMILPIYVSLERIDRRLVEAAQDLYSGPGRSFRKVVFPLSLPGVFAGSLL
                     TFIPASGDFINAELLGGPNQSMIGTVIQNQFLQVHDYPLAAALSFVLMAILSIGVMVY
                     ARMVGTEDLA"
     gene            complement(7179..8276)
                     /locus_tag="AC16JC_06220"
     CDS             complement(7179..8276)
                     /locus_tag="AC16JC_06220"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR5164327.1"
                     /translation="MSAIGRRRFVSRLAGMAGAAALDGCGRPAPGRSARPLRISQWPL
                     YIDDRTIPDFQRATGITVQYTQDINDNNEFFAKIEEPLKRGQSVDRDIVVLTDWVAGK
                     MIRRGYLAPLDDARFPNKVNLADYVRDADFDPERRYTVPWFSGMVGLGYDRRRVGRDI
                     TSVEDLFDARFKGRVTMLTEMRDTLGLVMLGMGKDPASCTLDDAKRAAARIAQARDSG
                     QIRAFTGNDYADDLASGNVLIALAWSGDIQGLRADNPDLQWIAPREGAILFSDNMMIP
                     KTSDRQDLAMAWIDWCYDPVHSAQIVSGAPYISDVKGAGAELAKIAPALAGSELVNPP
                     PELVQRLHVFRTLSDDEEAQFNRVFQRAIGA"
     gene            complement(8273..>8776)
                     /locus_tag="AC16JC_06225"
     CDS             complement(8273..>8776)
                     /locus_tag="AC16JC_06225"
                     /inference="COORDINATES: protein motif:HMM:NF019998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HIR5164328.1"
                     /translation="GLSDRIAVMNAGRVEQIGTPQEIYHSPATVFVAGFIGGANLLPA
                     TVAEVRGGLASVDLATGDHVELPAGDWGARTGRRAVVMVRPERLRLDAAAVPRGTLAA
                     VVQSVAFQGPVLRCDLRARDGTELVARVGPEQAVPDLRPGQELRVSWDVDAARLLPPS
                     ESGDSGS"
BASE COUNT         1391 a         3114 c         3033 g         1238 t
ORIGIN      
        1 ttcgagacgt cgtcgaagga cgccgtgatc atcttcaaga agcccacgcg cgacggcgtc
       61 cacatcgagg acgtgaagac cgtcgacaac gaatggctcg tcaccgggac ctcgggcgtc
      121 gtgctgatcg tcgtgggcct gggccccacg atgaaagcgg cgcagaagca ggtctacaac
      181 cggatcgaga acatcatgat ccccaacatg tactaccgca aggacatcgg ggaccgctgg
      241 ttcgaggatt tcgaccggct gcacacctgg ggcttcctgc gcgactgacc gatccggccc
      301 catgagcggc gacgccctcg agcacgctac gctgcgccgc gtgagccggc ggctcatccc
      361 gtttctcttc gtcgtcttca tcgccaacta cctggaccgc gtgaatgtca gcttcgcggc
      421 gctccagatg aaccgggacc tggggctctc cggcgccgcc tacggcctcg gcgccgggat
      481 cttctttctc ggctacgttc tgttcccggt ccccagcagt ctgctgctcg cgcgtgtcgg
      541 cgcgcggcgc tggatcgcgg ccatcaccac cgcctggggc ggcattgcgt cagccatgat
      601 gttcgtccgg ggaccggcca ccttctatct gctccgcttc ctgctcgggg tcgtggaagc
      661 ggggttcttt cccggcgtca tcctgtactt cacctactgg tttcccgcgg cgcaccgcgc
      721 ccgagcggtc gcgcgtttca tgaccgcgat ccccgtggcc cagatcgtgg gcggaccctt
      781 gtcggggtgg ctgctggggt tccacggccg ggccggcctc gccggatggc agtggctctt
      841 cctgctcgag ggcctgccct cgctcgccct gggctacctg gtgctgacct acctcacgga
      901 ccggcccgag gatgccacct ggctggcgcc ggaggcccgc gcctggttga ccgggcggct
      961 gcgggccgaa caccccgaag acgcggcacc ggcgcgaccg tggcgccggc tgctcgcgac
     1021 gccggcgctg tggcacctgt cgggcgtgtg gattctgatg gtgctcgtcg ggtacggcca
     1081 gctgctctgg ctgccccaga tcatcaaggg ggcctcgggt ctcagcaatc tagcagtcgg
     1141 ttttctctcg gccgccccgc ccatcgcggc ggcggtcgcg atggtgtgga ttgccgccca
     1201 ctccgaccac accggtgagc ggcggcggca cgtggccctg ccctgcctcc tcaccgcggc
     1261 gggcttcgcg gcaacagcag ccgcaccgcg cgcaccgctc ctggccaccg ccgcgctgac
     1321 gctcgtggcc atcggaatcg cgggatcgtt cgggccgttc tggggtctcg cgacgaccag
     1381 tttgagcgga ccggcgagcg cgggtggcat cgccctcgtc agctcgatcg gcaacctcgg
     1441 cggcttcgcg ggaccctacg tcgtgggcct gctcaaggac gcgacgggga gcttcgcggc
     1501 ggtgctggtc gtattcagcg gactcgccgc cgctgccggg ctgctggccc tgcgattccg
     1561 cccggccggg cggacatagc agtccccgag catggcccgg ttagattccc gcgaccgtga
     1621 tctcctctcc ccatgaatcc gccgtgatcg ccggcgcggg ccccatcggt ctcgcctgcg
     1681 ccatctcggc gcgccgtcgc gggctcgatc cgctgatcat cgatgccggc gcgctggccg
     1741 agtccatcgt gcgctacccg gtcggcatga ccttcttcac gacgccggag cggctcgaga
     1801 tcggcggcca cccgttggtg tgcgccggcg ccaaggccac gcgcgaggag gcgctcaagt
     1861 actaccgggg cgtggcgcgc gcggaggggc tgcgggtacg cccctatgcg cggctggtcg
     1921 gcgccgagca cagggacgga tgcatcgcgt gcgacatcga gacgcgactc ggcggcgagt
     1981 cgatcggctg cgaccgtctg gtgctggcca cgggctactt cgatcacccc aatgccctcg
     2041 atgtgccggg agaggcgttg ccccacgtcc atcactattt cgacgaggcg caccgctcgt
     2101 tcaaccagga tgtcgtggtc atcgggggca agaactccgc ggtcgaagcg gcgctgcagc
     2161 tgttccgcgc cggggcgcgc acgacgctgg tctatcgcgg cgccgagctg tcgcgcagcg
     2221 tcaagtactg gctcgcgccc gacctcctca accggatcaa ggccggagaa atcgccgcgc
     2281 gcttcggggc cgaggtcgtg gagatcacgg cgctgcatgt ccgcatccgc cgcgccgacg
     2341 gcacggtcga ggacctcccc gccgatcggg tgtacgcgct gaccggcttc cacccggact
     2401 tcgacctctg cacgaggatc ggcatcagct tcgatcccgc cagcgggcgg cccgcgctcg
     2461 accccgctac gctggaaacc agcgtgccgg gcgtgcatct ggcggggagc gtcaccgccg
     2521 gacgcaagat ctcggagatc ttcatcgaga acgggcgctt cgacggcgac aagatcttcg
     2581 gcgacgcggc ggcccgggcc gcggcgcagt cgcgctacgc ggggatcgac cgcccggctg
     2641 gcgagtgaga gattgaagac acgggagcac agagcaggga gcaggaccct cccgtcgcag
     2701 ggaacgtact gctccccgct ccgtgctccg ccttccgaaa ggacgtgatc catgatgagc
     2761 gccacgaccc gccccacccg ccgccccacc atgccgccct gcgcgcacgc gccgcggccc
     2821 tacaccggtc cgtcacgcgc cgaggtgctc gcgacccgga agcgctatgc caatccggcg
     2881 atcttcacga tctaccgcga gccgctcatg ctggtcgaag gccaccggca gtacctgttc
     2941 gacgagaccg gacggcgcta cctcgacctg ttcgccggga tcgtgacggt cagctgcggt
     3001 catgcgcatc cgaaagtcac cgagcgcgtg cagcagcagg tggcgacgct gacccacgcg
     3061 acgacgatct acctccaccc ccatatcggc gagttcgcca ggcggctcgc cgccacgctg
     3121 ccctccggac tcgatgtgac ctatttcgtg aattcaggga gcgaagcgaa cgacctggcg
     3181 gtgctgctgg cccggctcgc caccggccac aacgacgtga tcgcggtgcg gaacggctac
     3241 catggcggct ccccggcggc catggcgctc acctcccacc atacctggaa gttcccgggc
     3301 cagctcaaca ccggcgtcca tcacaccgtc tgccccgacc cctaccgcag cccctttacg
     3361 gggacgcccg agcagatcgc cacgcgcagc gtggaggaaa tgcgggacct catccgcttc
     3421 tcgaccccgg gcaagatcgc ggcgttcatc gcggaaccga tccagggcgt gggcggggtg
     3481 acccacgggg cgcccaacta cctaggggag gcgtacgcgc tcacgcggga gcacggcggc
     3541 ctgtgcatcg ccgacgaggt gcaaaccggg ttcgggcgta cgggcgatca ctactgggga
     3601 ttcgagaact ggggcgtggt tccggacatc gtcacgatgg ccaaggggat cggcaacggg
     3661 ttcccgctgg cggccgtgac gacgcgccgg gagatcgccg agcgcctcac gcagcggatt
     3721 cacttcaaca cctacggcgg caatcccgtc tccatggccg ccgggctcgc ggtgctcgag
     3781 gtgatcgagg aagaagggtt gcagcgcaac gcgggcgagg tgggcggtcg cctgaaggcg
     3841 gggctgcgtg agctcgccgc ccggcatccg ctgatcggcg acgtacgcgg catggggctg
     3901 atgctgggcg tggagctggt gaaggatcgc ggcaccaagg agcccgcacg ggaggccgcg
     3961 gcggcggtgt tcgagcggct gcgcgagcag ggcatcctgg tcggcaaggg cgggctgttc
     4021 ggcaacgtgc tgcgcatcaa accgccgctc tgcctcacgg cggcggacgt ggatttcgcg
     4081 ctggatgtcc tggaccaggt cctggccgag gtctagctcg acatcccgcc cccgatcccc
     4141 agccggtcga gcagccgcag cagcaatccc ggctcggcgc cggcatccac gcggcgcagc
     4201 gcgcgtttca cggagcgcac ggcccagggc cgcagcggtc cggggggaaa cagggccggc
     4261 ggccgccgca ccaggggcag gtcgagcagg tcgctcgggc gctccagcgc gaggtgggcg
     4321 agcacgcggc ccgcgatgcg ggtgggcccc agtccgagcc cgctgaaccc caggccgtag
     4381 agcagcctcc cgtccagcgc ctgcccgaag aacggggtga gccgggcggt gacgtcgatc
     4441 gggccacccc agccgtaggc ccactccagg ccggcgagcg cagggaaatg gcgccggaag
     4501 ctcgcgcgca ggatctcgta ctgcgcgggt gagtggtcgt ggtccggcgc gacgccgtcg
     4561 cgggagaagt acaccggctc gctcgtgccc cacaggatcc gatcgtcgcg cgtcagccgg
     4621 tagtagttga agaatcggcg cccgtcggtc acgccctgcc gctcgcgcca gccgatgacg
     4681 tcccgctggg cggcggtcag cggctcgctg gccagcatgt agccgtacag cgggatcatg
     4741 cgccgcgcga cctgcggcac gagccggtgg gtgtaggcgc cggtggtgag cacgacccgg
     4801 cgggcgtcca cgcgcccgcg cgtgctgcgc aaccgcacgc cgtcgcccag cgactcgatg
     4861 cccatcacat ggcagcgctc gtagacggtc acccccatcc gctcggcatc gcgcagcagt
     4921 ccgtccacga gcttcaccgg gtcgagcacg ccgccggccg aaacgcggac gccgcccagg
     4981 tacagcggcg aattcaccgc ccggcgtacc gcctccttgt cgagcagctg ataggactcg
     5041 acgcccagcc cttcggcggt ctccaccatg tggcggcacg cgacgacctg ccccggcgtc
     5101 agggccacga ccagccgtcc ggtcggctcg tagtcgcagt cgatgccgcg ctcccggaga
     5161 aacgcggtca gctcaacgag gttcgcctca cccaggcgcg cgagccggcg cgcctcgact
     5221 tcgccgaaat gccggatcgc caggccgtgc gagtgatcga tggtctcgag caacatgcca
     5281 gcgttgcgcc cgctcgcccc gtggccggcc agcccctgct ccagcaccac gacgtcacag
     5341 gcggagtcga gccgcttgag aaacaacgcc gtccagagac ccgcgagtcc ggcgcccacg
     5401 atggcgacgt ccgccgcccg ggaggccggc agcgccgtga cggtgcgctc gggccggcgt
     5461 gcgagccagt agcacgcctg ctcgatcgcg gggccacgcc cgaggaacgt cacggttgtc
     5521 gcacggccgc tcgcgcgcga cgctgctgcc acagcagggt ggcgccgagg agcccgaggc
     5581 tcaggacgag gatcgaggtc gccaggacgt tgatctgggg aggaaaggcg gcgcgacggg
     5641 ccccgtagac gtacaacggg taggtgaccg cggcccccga gttgaacagc gtgatgacga
     5701 agtcgtcgag cgacagggcg aagcacagca tcgccgcggc gacgatgccc ggggcgatca
     5761 acggcagcgt cacccgggtg aatgcgcgcc acggcgtggc ccccaggtcc agggccgcct
     5821 cctccagcgt ccagtcgaag ccccgcagcc gcgcacgcac ggtcagcgcc acatagctca
     5881 ccgagaacat gatgtgggcg atcacgatcg tcgcgaagcc catccgggcg ttcacggcga
     5941 ggaacagcga cagcagcgac gacccgagta cgatctcggg ggtcgtgagc gggaggacga
     6001 gcaggaggcc gaccgcaccg gagccgcgaa accggtagcg gcacagggcg agcgcgatca
     6061 tcgtgccgaa gatggtggcg gcgaccgtcg ccactgccgc gaccttgaga ctcaggagca
     6121 tcgcgtccac gagctgcggg tacgcgaacg ggtgccgcca cgcgtccagc gagaagctct
     6181 tccagacgaa gttgaaccga ccttcggggc ggttgaagct gtacagcacg atgacgaaga
     6241 tcgggaggaa caggtacagc agcaccaggg cagcggtcgc gtcgagcagc ccgccccagc
     6301 cgcggcggcg gctcccggcg ctcacgcgag gtcctccgtc ccgaccatcc gggcgtatac
     6361 catgacgccg atggagagga tcgccatcag gacgaacgag agggcggccg ccagcgggta
     6421 gtcgtgcacc tgcagaaact ggttctggat gaccgtaccg atcatgctct gattggggcc
     6481 gcccagcagc tccgcgttga tgaagtcgcc cgaagcgggg atgaacgtca gcaggctgcc
     6541 cgcgaacact cccggcagcg acagcgggaa caccaccttg cggaaggagc gccccggtcc
     6601 gctgtacagg tcctgcgccg cttccaccag ccgccgatcg atccgctcca ggctcacgta
     6661 aatcgggagg atcatgaacg ggaggaaatt gtaggtcagg cccccgatca cggcccagct
     6721 cgtgttgagg aggcggcctt ccgccgggag caggtgcagc cacccgagca accccacgac
     6781 gggaccgccg tcgttgagca tcgtcttcca ggcgaaggtg cggaccagga acgcggtgaa
     6841 gaaggggaga atcaccaacc cgagcagcag gtgccggagc cttccggccc ccgagcggaa
     6901 cgccatcaca taggccagcg ggtacccgag caggagggcg atcagggtcg caacgaacgc
     6961 aaaccgaaac gaccggatca ggaagctgcc gtacccggtg aaggcccggg cgtagccgtc
     7021 ccaccagggc gcgtcacctg ctccgagcga cgtgcgtgcg agcgcgagga tcggaacgag
     7081 gaagaacacc accagccaga gcacacccgg agccagcagg acgtacggcg cgagccggcg
     7141 ccgtcccggc gcgggcgcag cggctcccgc cggcgcagtc aggcgccgat cgcgcgctga
     7201 aacacgcggt tgaattgcgc ctcctcgtcg tcggacaggg tccggaacac gtgcaggcgc
     7261 tgcaccagct ccggcggggg attgacgagc tcgctgccgg cgagcgcggg cgcgatcttc
     7321 gcgagctccg cgccggcgcc cttcacgtcg gagatatagg gcgcgccgga gacgatctgc
     7381 gccgagtgca ccggatcgta gcaccagtcg atccaggcca tggcgaggtc ctgccggtcc
     7441 gaggtcttcg ggatcatcat gttgtcggag aagaggatcg cgccctcccg cggcgcgatc
     7501 cactgcagat cgggattgtc cgcgcgcaag ccctgaatgt cgccggacca ggcgagggcg
     7561 atcaggacgt tcccgctggc gaggtcgtcc gcgtagtcgt tccccgtgaa cgcccggatc
     7621 tggccgctgt cgcgcgcctg cgcgatcctc gccgccgccc gcttcgcgtc gtcgagcgtg
     7681 cagctggccg ggtccttccc catccccagc atgaccagcc cgagcgtgtc ccgcatctcg
     7741 gtcagcatcg tcacccggcc cttgaaccgc gcgtcgaaga ggtcctcgac gctcgtgatg
     7801 tcccggccca cccgccggcg gtcgtacccg agcccgacca tccctgagaa ccaggggacg
     7861 gtgtagcggc gctccggatc gaagtccgcg tcgcgcacgt agtcggccag gttgaccttg
     7921 ttgggaaacc tggcgtcgtc gagcggcgcg aggtacccgc gccggatcat cttccccgcc
     7981 acccagtcgg tgagcaccac gatgtcgcgg tcgacgctct gtccccgctt cagcggctct
     8041 tcgatcttcg cgaagaactc attgttgtcg ttgatgtcct gggtgtactg taccgtgatc
     8101 cccgtggcgc gctggaaatc ggggatggtc cggtcgtcga tgtagagcgg ccactgggaa
     8161 atccgcagcg gacgggcgct ccgtccgggc gcgggcctgc cgcacccgtc gagcgccgcg
     8221 gcgccggcca tgcccgccag acgactcacg aaccgccggc ggccgatggc actcacgatc
     8281 cagagtctcc ggattccgag ggagggagca gccgggcggc atcgacatcc cagctgaccc
     8341 gcagctcctg ccctggccgc aggtcgggaa ccgcctgttc cggacccacg cgtgccacga
     8401 gctcggtgcc atcccgggcc cgcagatcgc agcgcagcac cggcccttga aacgcgacgg
     8461 actgaacgac cgccgcgagc gtgccgcggg gcactgcggc ggcatcgagc cgcagacgct
     8521 ccggccggac catcaccacg gcgcgcctac cggttcgcgc cccccagtca ccggcgggca
     8581 gctcgacgtg gtcgccggtg gccaggtcga cggacgccag cccgccgcgc acctcggcca
     8641 ccgtcgccgg caggaggttg gcgccgccga tgaagccggc gacgaacacg gtggcgggcg
     8701 aatggtagat ctcctgcggg gtcccgatct gctcgacccg tcccgcgttc atcacggcga
     8761 tgcggtcgga cagacc
//
LOCUS       DBNGZW010000261        19557 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1183953, whole genome shotgun sequence.
ACCESSION   DBNGZW010000261 DBNGZW010000000
VERSION     DBNGZW010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 19557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19557
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1183953"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            50..649
                     /locus_tag="AC16JC_06230"
     CDS             50..649
                     /locus_tag="AC16JC_06230"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR5164329.1"
                     /translation="MALALVLALAGAGGLCGQQRGAAAADRYDHIFRKYTKRYFGPAF
                     DWRHFKAQGLAESGLDVTARSRDGARGIMQLMPSTMEAIQSSAPDLVSIDVPELNIAA
                     GIRHDRALWNIWERVATPLDRRRFMFASYNAGSRTIARAQRVARRHRLDPAQWENIRR
                     IAWRVWRWHQVQTLRYLRKIETIFGLLDTEGKVPDGRRW"
     regulatory      962..1050
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1152..1820
                     /locus_tag="AC16JC_06235"
     CDS             1152..1820
                     /locus_tag="AC16JC_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0061097 - regulation of protein tyrosine
                     kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YveK family protein"
                     /protein_id="HIR5164330.1"
                     /translation="MGVSELHDHLRIRPTPPPGEPGRAPLYRRWLRTGSWVVPVMLAL
                     SVGTALIVTSRQTPTYSSAATVALVPGPSVADGSDVLRSLETLERRTVIATFAQIPLA
                     DRIQQAAAHDISLPGDSLAGYRTLAFVIPNTNLIRIEVEGPDPTRTAALANALLTTTA
                     AEAKAMYRVFELTALARAVRPTAPVAPNPKRAVALAAILGMFLGLAATYLLVRPPRPS
                     VTPT"
     gene            1829..3337
                     /locus_tag="AC16JC_06240"
     CDS             1829..3337
                     /locus_tag="AC16JC_06240"
                     /inference="COORDINATES: protein motif:HMM:NF016800.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008754 - O-antigen ligase activity
                     [Evidence IEA];
                     GO_function: GO:0008755 - O antigen polymerase activity
                     [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HIR5164331.1"
                     /translation="MDGTPGRPGIAVAALPEWSGRGVVIAGAMAGAVAVGYLAASAPE
                     LLHFQASALDVAVLVAVLAFGAVVIWRPVLALGALILVTWLNLSDILIRWHGAPSVLQ
                     ALALPLLVAAWFVQRQRGFHRPLPATLTISLATYVLVLWLSTAWARDTALGELRALEV
                     TKAFVIFALVLLLAASREHIRLGAWTLVAAATCVGALGVFQVVAGRYGQTFAGLARVE
                     HAQIYGRVFEPRIAGPVGDPNFFAQILIPAVPIALQLAVSTSRLRERVAAFACAAVVT
                     AAVVMTYSRGGALALGVVLALWLVGQGASARRLAAAGVALAVLVAVLPSDFARRLATF
                     RQFVPRSADVLRLDSSFEQRRVLATTAWLMFLDRPLTGVGAGNYTRYYRPYTEETGSV
                     VLEYEDFDAPHYPHNLYLEIAAETGLPGLIVFGTALATCFLYLRRARLAFRRDGDAAS
                     AALATGVQLALIGYLVSSLFLHGHFPRYLWLLFGLCAALYRVAPRAPLPEAP"
     gene            3334..4791
                     /locus_tag="AC16JC_06245"
     CDS             3334..4791
                     /locus_tag="AC16JC_06245"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR5164332.1"
                     /translation="MSQRDLGRRTAWGFIWAMFGTGAGKVLTLASIAVLARLLAPAEF
                     GLFAFALVYVSYLETLGDLGSGTALIYWPDRTGEVAQVTFALNILTCLAWFLLTWLLA
                     PLVADFFHSPAGAPILRALGATFVLKGLGNTHDALCKKELRFRARLVPEVGLAAVKGA
                     LTIALALGGFGVWSLVWGHLVATGARTALLWLVVPWRPAWRWPAQLVRPVLGYSVWIV
                     VVNVVAAIVHHVDYLVVGRMLGVTALGFYQMAYKVPETLVILLVWQTSMVLFPAFATA
                     RASGGSVADTYLTAFRYFSLLAVPMAVAVFVLAEPLVLALFGESWRPSIPILKALAIY
                     TGLRGLGTNAGDVVKAHGQPKLLAAVAILKALLLVPALVVAARWGASAVAATTAGTAG
                     LLLLLDFGLLRRVAGIRFRAVAAAFAPSLPPAAVLAVVLTGWVHAARDFGSLVVLAGG
                     AVLGLVGYLLAVRALSPDLYRRAVSSLRPQPAAVP"
     gene            4788..5948
                     /locus_tag="AC16JC_06250"
     CDS             4788..5948
                     /locus_tag="AC16JC_06250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5164333.1"
                     /translation="MSDRPLRPLVLLSHLATAGAERVTVSFVRRLRAMGIEATVCTLT
                     TSWDGLLAAELEASGVRRYDLGARRLADPRALLRLHRLLARERPDLVHAHGQDAAILA
                     AAARRLRPFPLVITRHVMEDAMASLRQRLRARAGWAALRRADAAVAVSRAVAGRLAEL
                     SGLPREAIHVITNGVELERFAADVASSRLELRAAFGAGPEDRLVLVPAALRPGKGHDV
                     LLDALPALRARAPGVRLLFAGGGDQEAALRARAGALGDGRGSCDAVLFLGPRSDIPDL
                     LAACDLVVLPSEAEALPTALIEAAASGRPAVATRVGGIPEVVADGVTGLLVRPNDPAA
                     LFEAMVALLLDGERARSYGIAARWLARQHFAIEKQVERTLTLWDSIRPRGHG"
     gene            5945..7114
                     /locus_tag="AC16JC_06255"
     CDS             5945..7114
                     /locus_tag="AC16JC_06255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5164334.1"
                     /translation="MRITWSVPAPGERLHGSRGDVVRARHFIEALRGEGHEVRLVERA
                     AAPTTAAIVSTYRRVVRRLIPRKPALALRDAGRWAEALAHGRRVAAAAREQGADVIVE
                     TQVHLAGSGVLAARLTGLPLLLDDCSPSSEERVLGAGFPQLVRRVFRSQFRAARHLVV
                     SSHALRARLSDEGVPADKLHVVPNGVDPDAYDHVDRDAVRARLGFAEACVIGFVGSFQ
                     PWHRVGLLVEALALLHEERAVRLLLLGDGPQRPAVLSAVRRLGLDRLVTAPGALAPEE
                     LPAHVAACDIGVVPASNDYGQPMKLMDYAAAGLPAVAPDLAPVREIVQDGVTGVLFPP
                     EDVRSLSLALARLVRNPALRRAMGARARRIAEDGSWRVRARQLVSLVSAPVVALA"
     gene            7127..7795
                     /locus_tag="AC16JC_06260"
     CDS             7127..7795
                     /locus_tag="AC16JC_06260"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR5164335.1"
                     /translation="MYLTATIDDAQPLAPTFANAAPAALSRLKRVLDVAVVGALLVLL
                     APLLVAIAIAIKLTSPGPVLFVQRRIGYGGTVFPMLKFRTMYVGADQAEPLLAARQRD
                     RTFLKIENDPRATSLGRWLRLTSLDELPQLLNVLRGEMSIVGPRPILLCDLAKYPRGH
                     EMRRFTAKPGITGLWQVSGRSRCTDRERIQLDLVYVDCWSLWLDLLILARTLPVVLAG
                     QGAY"
     gene            7798..10113
                     /locus_tag="AC16JC_06265"
     CDS             7798..10113
                     /locus_tag="AC16JC_06265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5164336.1"
                     /translation="MSRAFEQGTTTAVPAQPARGDVGAERARSAPAGPVPVRSAIAVL
                     LSRFPLVTETFILREIEELERQGQPVRLVPLMQERPAVLHREAQAWLPRALFTPFLSR
                     EILAANARAFARRPLRYLLLLGRILLGSLRSWNVVIRSVALFPKCVYLAERLEREGIR
                     HAHAHYATHPATAAFIISSLAPITFSFTVHAHDLFVAWRRPLLGAKVRRACFIRVISE
                     FNQSFLRALYPDCATKIRVIHMGIDPDAFQPLGPAPAAGGAAPGRLVCVGALQPYKGI
                     PVLIEACRLLATAGVRFECDVVGEGWLRSSLEESIARHGLRDHVRLRGALRQDEVAAL
                     LREAALVVLPSVVARDGQMEGIPVALMEAMAAARPVVASAISGIPELVDHGLNGLLVE
                     PGNAAALADAIATLLADPDRRRKMGGHGREKVLRAFRLDTVTQALRRELDKWTPPAGD
                     VPGLRDLAHAAGFAGHELGVRGVHGGRDSSVVEVMAADGLRTQELILKTHRTRALESR
                     VPAERARREYDVLVHLGHRFSAGKDSESKPSGKGFSLRTPRPLAIRMTDASLVMERCT
                     GDRLDDLIRRARRDPDGEHWTALEHAVHGTGQWVRRLHEVTRRADPPGVQRAWNVQID
                     AALRDAVASPAGEETRQRLEALRRHGPPQVDLVGCHGDLSPGNILASSSSVVVLDFEG
                     YHEGLPWEDVAYFLLHLELFFPFAWRAGRFALLRDAFLAGAGVSSDPVGLELCRTVAA
                     LRALAHVHVRSWRFWPHAALLRRVTLNGGRV"
     gene            10113..12161
                     /locus_tag="AC16JC_06270"
     CDS             10113..12161
                     /locus_tag="AC16JC_06270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5164337.1"
                     /translation="MLEVIFWVALLVVVYAYAGYGVVVYCLVRIARLLRPSVPHPVVA
                     AEAPEVTLVVPAWNEAAWIGAKIANCLELDYPRDRLKILVVTDGSTDDTAEIARRFAG
                     VEVLERRERVGKAAAINRAMEWVTSPIVVLSDANTMLNCEAVRELVRHYADASVGAVA
                     GEKRIRRRDSAAAAGEGFYWRYESRLKRLDSELWTVVGAAGELLSYRRELFTPLEEDA
                     VQDDFLLSLRIAARGYRVVYESRAYATEEASMTIAEELTRRIRIAAGGWQSMVRLRSL
                     LDPRRHPLLTFQYVSHRVLRWSVVPVLLLLLLPVNAVLATGSGPLAPLYRVLLAGQLA
                     FYAFALAGYLCDRRGLRLRALVPPYYLVVTNYAVLRGLGRFLAGRQPATWERARRAAL
                     LLLALGWASSLPAQSRDAAVHGQFTVDAVHFDNLFEASDGQPRQTLWGQRVQGRLSAP
                     LGARGVAIYGEGDGMRIPGFAPAYGAGLGVQRLGGVTTFDAKVSYLGNRPAAEIGSGL
                     GPANLLRFDGEGAYRPFSAVQLVGRGQARREQYTSQVMRNGTAFEFGPLVRFRAFGSG
                     FSPEAGAMFGQRDGRDPNLAYRERGYFVGASSLTFGYRLYLSARYRWRTRSYSSEDPL
                     LGNFGRVDRRQQVSVAVAVTLLPGLSWVTYYAHERGVSTRPGENFTTGFLSVGLATKF
                     "
     gene            12163..12972
                     /locus_tag="AC16JC_06275"
     CDS             12163..12972
                     /locus_tag="AC16JC_06275"
                     /inference="COORDINATES: protein motif:HMM:NF016908.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HIR5164338.1"
                     /translation="MRRAVKRLLAHSHPAYRWARRLSMFVRYGCGRPHEADFAGFRHF
                     GRRSGLFLDIGANSGESVLSFRLFNATTRILSIEPNRYHEPDLRFLKRWVRRFDYRLW
                     AAGDENGWATLYVPVYRKLPLTGEASFHREQALGNYWMHEQLGGNGGAKEGVRLREVP
                     VEVKRLDDLRLKPAFIKMDVQGFEAKVVAGLHDTISASRPVLLIERSGCDAALLARLA
                     DLGYTAFLYVPARDAFAPWRDEPAQNLFFIPGHTSIPMTAPPPLEQLEVVG"
     gene            12969..14423
                     /locus_tag="AC16JC_06280"
     CDS             12969..14423
                     /locus_tag="AC16JC_06280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164339.1"
                     /translation="MTGTTPRVVGSRVVVCLMGLVAVASQRAAAQELRVAVGEREIAR
                     VAVSETRGYPALPIDLLEPLGAKVVVGKDVVQVRLFGDTLRFYPGYAEFQIDVRTESL
                     QALTYLQDGTLYVAQWFFTYWLPNHYPWRLTYGDHVLRLTAGAVAASGGSDIAAARAV
                     RESPPAAPAARAARRAPPPADTARSEAVADSLDAERPVTRDRTYDPDEPLGEILGFID
                     ARVSGVYDSNIDHRTAATRSYGTVGRLGVGIQSSPVRPFLMARYDFALYRFANSDQWK
                     RTTHDVSADVAPTFGAVRLRLGAALRLGSWTEDRQPANQIVVRPQIEFRPTPIHLINL
                     YVMQSARRIDVGTTTLRDTFRLAGLGYYLWWHAGGFRVDARYESDKSEYVPSSYDGWT
                     GYAWTRIPFTESFRVTLESAYNRRRYAHSFVDPAHTIMRRDRRWTLTTSFTTLLPTPG
                     WEVSLVYTFEDGRSNSPSAVYRAHRVETMVRRRW"
     gene            14466..17732
                     /locus_tag="AC16JC_06285"
     CDS             14466..17732
                     /locus_tag="AC16JC_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPS10 domain-containing protein"
                     /protein_id="HIR5164340.1"
                     /translation="MRHRRPRPFAARVPVLTFLLVTSPAATLVAQRRAAPSSSGGGAA
                     AIDTARYAQLHFRHIGPEGNRVSSVAGVAGDPGTYYAGAASGGLWKSGDGGVHWDPVF
                     DDQPVSSIGALAVAPSDPNVVWAGTGEPFIRSNVSVGWGVYKSTDAGKHWTKMGLDST
                     GRISRIVVHPTNPDIVYVAALGHLYGPQPERGIYRTSDGGRTWERVLFVNDSTGASDL
                     VMDPNNPRILFAGFWQVELHTWIRSSGGTGSGIWTSRDGGTTWTRVTGSGLPTKPVGK
                     IGLAMTRANSNRIYALIETGDGVPAVNIAQPDTGRLFRSDDGGASWQLVSQDRQVAGR
                     THYYNRMAAAPDNDNEAYFLTASWAKTLDGGQTIIDPPNAQVPGGDHHDIWIDPTNGN
                     RMIVSHDGGVSITANRGRSWLRVQLPIAQVYHVTLDNRVPYYVYGNRQDGPSSRGPSN
                     SRTGGDIPRGMWGTVGGGESGWATPDPVDPDIVWSSASGFGSVGGIVSRYDVRTGIAR
                     STEIWPQATIGWPAGDLKYRFVWTFPLTISPHDHNTVYVGSQHVHVTSDGGVTWKEIS
                     PDLTRNDKSRQVPSGGLTPDNIGVEYAGVVFAIAESPLKRGLIWAGTNDGKVQLTRDG
                     GGHWADVTGNIKGLLDWGTISSIEPSRYDTGTAYIAVDGHQVNSRDPWIYRTTDYGAS
                     WTPITTGLPKSPLGYVHIVKEDPVRRGLLYAGAENGLYVSFDDGAHWQPLQNNLPHAP
                     VYGIAVQPQFHDLAVATYGRGFWILDDITPLRALTPAVTASDAYLFAPRSAYRFRGIE
                     GPYEASDDPVAGTNPPYGASLDYWARTAPRDSAKDSVTIRIVDAGGKVVRTITQLPAA
                     GVNRLWWDLRYEPTPEAKLRTSPLYAAWLAVKPEGTPAPGIGRFSLLAPPGRYTVRLT
                     AGGKSFEQPLDVLKDPNTGASDDDLRAQGVMMDAVLADLDSTVAMINRVELLRGQLAS
                     LRATLAGDSTAADVRARADTLDRALRAFEEDLFQVKVTGRGQDLLRWPMKLAEQLMYL
                     AQSLTSSDFAPTGPQRDVQQLLAGQVRESRTRLDRLMAGDVATFRQFLRSRNLPNLIP
                     "
     gene            18043..18813
                     /locus_tag="AC16JC_06290"
     CDS             18043..18813
                     /locus_tag="AC16JC_06290"
                     /inference="COORDINATES: protein motif:HMM:NF022750.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HIR5164341.1"
                     /translation="MSVIPVVLRRRIVAVAPLAGALAIGPASLRGQQAATVVPGVVVH
                     MDAAPGPDTVVFRRTITRGGVDSITGTRTVVSRVVPGQGGARLLEVEQRFPGGGGEIV
                     DTALADLGTLRAVAHRSHQPRRTMRFDFVDGAAEGSLSDSGAVRQELGGPIFDSNVIE
                     LVVAALPLDARFSAELPFFIYERGGRVPMAVAVRERARVAFPMLGEREAWIVTVGVPG
                     APATIWVDTATRAVLRTRYDITARAMSLTDDRVTPLHG"
     gene            complement(19027..>19557)
                     /locus_tag="AC16JC_06295"
     CDS             complement(19027..>19557)
                     /locus_tag="AC16JC_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164342.1"
                     /translation="EATRRAPGATVQPLLHMKGFLYISREAWARLISGPPMAYGLREY
                     LGERNKQLESGEGVGEQAMADAMQRIGATIINAVLDSLPDSARACPRPQHCPGLRWVF
                     YLQIGSPNQDYRSMAMDGEAATLVTSWTALYGMPDFVLLTGLAAWPETQAQLDRLLPP
                     PDSRLLAAAWWVRMSL"
BASE COUNT         2506 a         6951 c         7028 g         3072 t
ORIGIN      
        1 tcgtagcgat ggcgatcgtc attggcgggg cgctcaactg acgcggtgcg tggcgctggc
       61 gctcgtcctg gcgctggccg gcgccggggg cctgtgcggc cagcagcgcg gtgctgcggc
      121 ggccgaccgc tacgaccaca tcttccgcaa gtacaccaag cggtatttcg ggccggcatt
      181 cgactggcgg cacttcaagg cccaggggct ggccgagagc gggctcgacg tgaccgcgcg
      241 cagccgcgac ggggcgcgcg gcatcatgca gctcatgccg tccaccatgg aggcgatcca
      301 gtcgagcgcc cccgatctcg tctcgatcga cgtccccgag ctgaatatcg cggccggtat
      361 ccggcacgac cgcgcgctgt ggaacatctg ggagcgggtg gcgacgccgc tcgaccggcg
      421 ccgcttcatg ttcgcgagct acaacgcggg gagccgcacc atcgcgcggg cccagcgcgt
      481 ggcgcgccgc caccggctcg acccggcgca gtgggagaat atccggcgga tcgcctggcg
      541 cgtgtggcgc tggcaccagg tgcagacact gcgctacctg cggaagatcg agaccatctt
      601 cggcctgctg gacacggagg ggaaggtccc cgacgggcga cgctggtagc gagccgcccc
      661 aagtcggtgc acgcattcgc tcccgcagcc ccaaggtgat gctgcaaccc gcgcgcggaa
      721 cctcgacagg caggcacggg acctgcctat cgggcgagtg gtcgggggcg gaccggggct
      781 cgtggtggcg accccgcccg ccggccggga cgcgtgggcg aggccgcgct ggcggtgcgt
      841 gaccggtgaa acggtgcgct tcgtcacgta acctctcggg acccacgggc atcttcaccg
      901 caggttgcgg tgcaccatct ccgcggggtg gacagggtcc cgtggggttc aacgagcgaa
      961 tcgctgctca gagcaaaacc gtcgaaaggc ggtgacgcag agtcacgggg ctacagcggc
     1021 gccagccgcc acgccagccg ggccgccagc gagtcagact gctggtggcg cttttccgtt
     1081 ttcgaaggtc ccaccagtac cgtcagccca gtttccagta ccacgacaat gtcaggcaag
     1141 gaggttccca gatgggagtg tcggaactgc acgaccacct ccggatccgg cccacgcctc
     1201 cgccggggga acccggtcgc gcgccgctgt atcgccgctg gctgcgcacg gggtcctggg
     1261 tcgttccggt catgctggcc ctgtcggttg gcacggcgct gatcgtgacc tctcgtcaga
     1321 ccccgacgta cagcagcgcc gcgaccgtcg cgctggtgcc ggggcccagc gtcgccgacg
     1381 gctcggacgt gctgcgcagc ctcgagacgc tggagcgtcg caccgtcatc gccaccttcg
     1441 cgcaaatccc cctggccgac cggatccagc aggcggcggc gcacgacatc tcgctgccgg
     1501 gtgattcgct cgccggctat cgcacgctgg cgttcgtgat tcccaacacg aacctcatcc
     1561 gcatcgaagt cgagggaccc gacccgacgc ggaccgccgc gctcgcgaac gcgctgctga
     1621 ccacgacggc ggcggaggcg aaggcgatgt accgggtctt cgagctgacg gcgctggcgc
     1681 gcgccgtccg gccgacggcg ccggtggcgc ccaacccgaa gcgcgccgtc gccctggccg
     1741 ccatcctcgg catgttcctc ggtctcgcgg cgacgtacct gctcgtgcgc ccgccgcgtc
     1801 cgtcggtcac gccgacctga cgcgggcgat ggacgggaca ccgggtcgtc cggggatcgc
     1861 cgtcgccgcg ctccccgagt ggagcgggcg tggcgtcgtc atcgccggcg ccatggccgg
     1921 cgccgtggcc gtgggctacc tggcggccag cgcgccggag ttgctgcact tccaggcgtc
     1981 ggcgctcgac gtcgccgtgc tggtcgccgt cctggcgttc ggcgccgtgg tgatctggcg
     2041 gcccgtcctg gcgctggggg cgctgatcct ggtcacctgg ctcaacctgt ccgacatcct
     2101 gatccgctgg cacggcgccc cttcggtgct gcaggccctg gccttgccgc tgctcgtcgc
     2161 ggcgtggttc gtccagcggc agcggggctt ccaccggccg ctgccggcga ccctcaccat
     2221 ctcgctcgcg acctacgtgc tggtgctgtg gctctcgacc gcgtgggcgc gcgataccgc
     2281 gctgggagag ctgcgggcgc tcgaggtcac gaaggcgttc gtgatcttcg ccctcgtcct
     2341 cctgctggcg gcgtcgcggg agcacatccg gctgggcgcc tggacgctgg tggccgccgc
     2401 gacctgcgtc ggcgcactcg gggtctttca ggtggtcgcg ggccggtatg ggcagacctt
     2461 cgcgggcctc gcccgcgtcg agcacgcgca gatctacgga cgcgtgttcg agccgcgcat
     2521 cgccggtccc gtgggtgatc ccaacttctt cgcccagatc ttgattcccg cggtccccat
     2581 cgcgctgcag ctcgccgtca gcacgtcgcg cctgcgcgag agggtcgccg cgttcgcctg
     2641 cgcggcggtc gtgacggccg ccgtcgtgat gacgtactcg cgcggcggcg cgctcgcgct
     2701 cggagtggta ctggcgctgt ggctcgtggg gcagggggcg tcggcccgac gccttgcggc
     2761 ggccggcgtg gcgctcgccg tgctggtggc ggtcttgccg tccgacttcg cccgccgtct
     2821 cgccaccttc cggcagttcg tgccgcgcag cgccgatgtc ctgcgcctcg actcgtcgtt
     2881 cgagcagcgc cgcgtgctgg ccacgacggc gtggctcatg ttcctcgatc ggcccctgac
     2941 cggcgtgggg gcggggaact acacgcgcta ctaccgcccc tacaccgagg agaccggctc
     3001 cgtcgtcctg gagtacgagg acttcgacgc gccccattat ccgcacaacc tgtatctcga
     3061 gatcgccgcc gaaaccggcc tgccggggct gatcgtcttc ggcacagcgc tggcgacctg
     3121 tttcctctat ctgcgccgcg cgcggctggc cttccggcgc gacggcgatg cggcctccgc
     3181 ggccctggcg acgggcgtgc agctcgcgct gatcggctac ctcgtctcca gcctgttcct
     3241 ccacgggcac ttcccgcgct acctctggct gctcttcggc ctgtgcgccg cgctgtaccg
     3301 cgtggcgccg cgggccccgc tcccggaggc gccgtgagcc agcgcgacct cggccggcgc
     3361 acggcctggg gattcatctg ggcgatgttc ggcaccggag ccggcaaggt gctcacgctc
     3421 gcgagcatcg ccgtgctcgc gcggctgctg gccccggcgg agttcggcct gttcgccttc
     3481 gccctcgtgt acgtctcata cctggagacg ctcggcgacc tgggcagcgg caccgccctc
     3541 atctactggc ccgatcggac cggtgaggtc gcacaggtca cgttcgctct caacatcctg
     3601 acgtgcctcg cgtggttcct gctcacctgg ctgctcgcgc cgctcgtcgc cgacttcttc
     3661 cacagccccg cgggcgctcc gattctccgc gccctcggcg ccaccttcgt gctcaagggc
     3721 ctggggaaca cccacgacgc gctctgcaag aaggagctcc gcttccgggc gcgactggtc
     3781 cccgaggtcg ggctggcggc ggtcaagggc gcgctgacga tcgcgctcgc gctcggcggg
     3841 ttcggcgtgt ggagcctcgt ctggggccat ctcgtcgcga ccggcgcacg cacggcgctc
     3901 ctctggctcg tcgtcccctg gcgtcccgcg tggcgctggc ccgcgcagct ggtccgaccg
     3961 gtcctcggct acagcgtctg gatcgtcgtg gtgaacgtgg tggcggccat cgtccaccac
     4021 gtggactacc tcgtcgtggg gcgcatgctg ggggtgacgg cgctcggctt ctaccagatg
     4081 gcctacaagg tccccgagac gctcgtcatc ctgctcgtct ggcagacgag catggtgctg
     4141 ttcccggcat tcgccaccgc gcgcgcatcg ggcggcagcg tcgccgacac ctatctcacg
     4201 gcgttccgct acttctccct cctcgccgtg ccgatggcgg tcgccgtctt cgtcctggcc
     4261 gagccgctcg tcctcgcgct gttcggggag agctggcgcc cctccatccc gatcctcaag
     4321 gcgctggcca tctacacggg gctgcgcgga ctgggcacga acgccggaga cgtcgtgaag
     4381 gcgcacggtc agccgaagct cctggccgcg gtggccatcc tgaaggcgct gctcctcgtg
     4441 ccggcgctcg tcgtcgcggc gcgctgggga gcctccgccg tcgccgccac cacggcggga
     4501 acggcgggcc tgctcttgct gctcgacttc gggttgctgc gtcgcgtcgc cggcatccgc
     4561 ttccgcgcgg tcgccgccgc gttcgccccg agcctgccgc cggccgccgt gctcgccgtc
     4621 gtgctcacgg ggtgggtgca cgcggcgcgt gacttcggca gcctcgtcgt gctcgccggc
     4681 ggcgcggtgc tcgggctcgt cgggtacctg ctcgccgtgc gggcgttgag tccggacctc
     4741 tatcgccggg ccgtttcgag tctgcgtccg cagcccgccg cagtcccatg agcgaccgtc
     4801 ccctgcgccc cctcgtcctg ctcagccacc tcgccacggc cggcgccgag cgcgtgaccg
     4861 tgtcgttcgt gcgccggctg cgggcgatgg ggatcgaggc gacggtctgc accctgacga
     4921 cgtcgtggga cggcctgctc gcggccgagc tggaggcgag cggcgtacgg cgctacgacc
     4981 tcggggcccg gcggctcgcc gacccgcggg cgctgctgcg gctgcaccgg ctgctcgcgc
     5041 gggagcggcc cgacctggtg cacgcgcacg gccaggacgc cgcgatcctc gcggccgcgg
     5101 cccggcggct gcgccccttc ccgctcgtga tcacacgcca cgtgatggaa gatgcgatgg
     5161 cgagcctgcg ccagcggctt cgcgcccgcg ccggctgggc ggcgttgcgg cgcgcggacg
     5221 ccgccgtcgc cgtgtcgcgc gcggtggccg ggcggctggc cgagctgagt ggcttgccgc
     5281 gcgaggcgat ccacgtcatc acgaacggcg tcgagctgga gcgcttcgcc gcggacgtcg
     5341 cctcgagccg gctggagctg cgcgccgcct tcggcgccgg tccggaagac cgcctcgtgc
     5401 tggttcccgc ggcgctgcgg ccggggaagg ggcacgacgt cctgctcgat gcgctgccgg
     5461 cgttgcgggc gcgggcgccg ggagtgcggc tgctgttcgc gggcggaggc gaccaggagg
     5521 cggcgctgcg tgcgcgcgcg ggcgccctgg gcgacgggcg ggggtcgtgc gacgcggtgc
     5581 tgtttctggg gccgcgcagc gatatccccg acctgctggc ggcgtgtgat ctggtggtcc
     5641 tgccgtcgga ggcggaggcc ctgcccacgg cgctcatcga ggcggccgcg agcggtcgtc
     5701 ccgccgtcgc cacgcgcgtg ggcggcatac cggaagtcgt agcggacggc gtgaccggcc
     5761 tgctggtgcg tcccaatgat ccggcggcgc tgttcgaggc gatggtcgcc ctgctcctcg
     5821 acggcgagcg cgcccgctcg tacggcatcg ccgcccggtg gctcgcgcgg cagcacttcg
     5881 ccatcgagaa gcaggtggag cgtaccctga cgctgtggga ctcgattcgg ccgcggggcc
     5941 acggatgagg atcacctgga gcgtcccggc gccgggcgag cggctccacg ggagccgcgg
     6001 ggacgtggtg cgcgcgcgcc acttcatcga ggcgctgcgc ggcgaggggc acgaggtacg
     6061 gctggtggag cgcgcggcgg cgcccacgac cgccgcgatc gtttcgacct accgtcgggt
     6121 cgtgcgccgt ctgatcccgc ggaagcccgc gctcgccctg cgtgacgcgg gccgctgggc
     6181 cgaggcgctg gcgcacggcc ggcgcgtcgc ggccgcggca cgagagcagg gggcggacgt
     6241 catcgtcgag acgcaggtcc atctcgcggg ctccggggtc ctggccgcgc gactcaccgg
     6301 cctgccgctc ctgctcgacg actgctcccc gagctcggaa gagcgggtgc tcggcgccgg
     6361 cttcccgcag ctcgtccggc gcgtgtttcg ctcgcaattc cgcgccgccc gccatctggt
     6421 cgtctcctcg catgcgctcc gcgcgcgcct ctccgacgag ggcgtgccgg cggacaagct
     6481 ccacgtggtt ccgaacggcg tggacccgga cgcctacgac cacgtggacc gtgacgccgt
     6541 ccgcgcacgg ctcggtttcg ccgaagcgtg cgtgatcgga ttcgtcggtt cgtttcagcc
     6601 gtggcaccgg gtggggctcc tggtcgaggc gctggcgctg ctccacgagg agcgcgcggt
     6661 tcgactgctg cttctggggg acggcccgca gcggccggcc gtgctgtcgg ccgtgcggcg
     6721 gctcgggctc gatcgtctgg tcacggcacc gggcgccctc gcgccggagg agctgcccgc
     6781 ccacgtggcc gcgtgcgaca tcggcgtcgt gccggcgagc aacgactacg gccagccgat
     6841 gaagctgatg gactacgcgg cggccggcct ccccgcggtc gcccccgacc tcgcgccggt
     6901 gcgggagatc gtgcaggacg gcgtcaccgg ggtgctgttt ccgccggagg acgtccggtc
     6961 cctgtcactc gcgctggcgc gcctggtccg caatccggcg ttgcgccgcg ccatgggcgc
     7021 ccgcgcgcgc cgcatcgcgg aggatggcag ttggcgcgtg cgtgcccggc agctcgtttc
     7081 gctggtgagc gccccggtcg tggcccttgc ataaggaaag gacgtcatgt acctgactgc
     7141 aacgatagac gacgcacaac cgctcgcacc gacgttcgcc aacgcggcgc ccgccgcgct
     7201 gtcgcggctg aagcgcgtcc tcgacgtcgc cgtcgtgggc gccctgctgg tgctgctggc
     7261 gccgctgctc gtggccatcg cgatcgccat caagctgacg agccccggtc cggtgctgtt
     7321 cgtgcagcgg cgcatcggct acggcggcac cgtcttcccg atgctgaagt tccgcacgat
     7381 gtacgtcggg gcggaccaag ccgagccgct gctcgctgcg cggcagcgcg accggacgtt
     7441 tctgaagatc gagaacgacc cgcgggcgac ctcgctgggg cgctggctgc ggctcaccag
     7501 cctggacgag ctgccgcagc tcctcaacgt gctgcgcggc gagatgagca tcgtcgggcc
     7561 gcgcccgatc ctcctgtgcg atctcgcgaa atatccgcgt ggccacgaga tgcgccgctt
     7621 caccgcgaag cccggcatca ccggcctgtg gcaggtcagc ggccgcagcc gctgcaccga
     7681 tcgggagcgg atccagctcg acctggtcta cgtggattgc tggtcgctgt ggctcgacct
     7741 gctgattctc gcccgcacgc tgccggtcgt gctggccggc cagggcgcgt actgacggtg
     7801 agtcgcgcgt tcgagcaggg cacgacgacg gcagtccccg cgcagcccgc gcggggagac
     7861 gtgggagccg agcgcgcgcg ctcggcgccg gccggcccgg ttccggtgcg gagcgcgatc
     7921 gcggttctgc tgtcgcgctt ccccctcgtc accgagacgt tcatcctgcg ggagatcgag
     7981 gagctggaac gccaggggca gccggtgcgg ctcgtgccgc tcatgcagga gcggcccgcg
     8041 gtgctgcacc gggaggccca ggcgtggttg ccgcgcgcgc tgttcacgcc gttcctctcg
     8101 cgggagatcc tggcggccaa cgcgcgggcg ttcgcccggc ggccgcttcg ctatctcctg
     8161 ctgctgggtc gcatcctgct cggctcgctc cgcagctgga acgtggtgat ccgctcggtt
     8221 gccctgttcc cgaagtgcgt ctatctcgcc gaacgcctgg agcgcgaggg gatccggcac
     8281 gcgcacgccc actacgccac ccatccggcg actgcggcgt tcatcatttc gtcgctcgca
     8341 ccgatcacct tcagcttcac cgtgcacgcg cacgacctct tcgtggcctg gcgccggccc
     8401 ctgctcggcg ccaaggtgcg gcgcgcctgc ttcatccgcg tgatctcgga gttcaaccag
     8461 tccttcctgc gcgcgctcta tcccgactgc gcgaccaaga tccgcgtcat ccacatgggg
     8521 atagaccccg atgcattcca gccgctgggg cccgcgccgg cggcgggagg cgcggcgccc
     8581 gggcgcctgg tgtgcgtggg cgcgctgcag ccttacaagg gcatcccggt gctgatcgag
     8641 gcgtgccggc tgctcgccac cgccggggtc cggttcgagt gcgacgtggt gggggaggga
     8701 tggttgcggt cgtcgctcga ggagagcatc gcgcgccacg gcctccgcga tcacgtgcgc
     8761 ctgcgggggg ctctgcgcca ggacgaggtc gccgcgctgc tgcgggaggc cgcgctcgtc
     8821 gtgctcccca gcgtcgtggc ccgcgacggc cagatggaag gcatcccggt cgccctcatg
     8881 gaggcgatgg cggccgctcg gccggtggtc gcctcggcga tctcggggat tcccgagctc
     8941 gtggatcacg gcctcaacgg gctgctggtg gagcccggca acgccgccgc gctcgccgac
     9001 gcgatcgcca ccctgctcgc cgatccggat cggcgccgga aaatgggcgg ccatggtcgg
     9061 gagaaggtgc tgcgcgcgtt ccggctcgat accgtcaccc aggcgctgcg ccgggagctc
     9121 gacaagtgga ccccgcccgc gggcgacgtc cccgggctcc gagacctcgc ccatgcggcc
     9181 gggttcgccg ggcacgagct gggcgtgcgt ggcgtgcacg ggggacgcga ttccagcgtc
     9241 gtcgaggtga tggcggccga cggccttcgg acccaggagc tgatcctcaa gacgcaccgc
     9301 acgagggcgt tggagtcgcg cgtccccgcc gagcgggccc ggcgggagta cgatgtcctc
     9361 gtccacctgg gccaccgctt ctcggcgggg aaggactccg agtcgaagcc gagcggcaag
     9421 gggttctcgc tgcggacgcc ccgcccgctg gcgatccgca tgaccgacgc gtcgctcgtg
     9481 atggagcgct gcacgggaga ccggctcgac gacctgatcc gccgcgcgcg ccgtgatccg
     9541 gacggcgagc actggacggc gctcgagcac gccgttcacg ggacgggaca gtgggtgcgt
     9601 cggctccacg aggtcacccg gcgtgccgat ccgccgggcg tgcagcgggc gtggaacgtc
     9661 cagattgacg cggcgctgcg cgatgccgtg gcgtctcccg cgggggagga gacccggcag
     9721 cgcctcgagg cgctgcgccg gcacggtcca ccccaggtgg acctggtcgg gtgtcacggc
     9781 gacctctctc ccggcaacat cctggcatcg tcctcgtcgg tcgtcgtcct ggatttcgag
     9841 ggctatcacg aagggctgcc gtgggaggac gtcgcctact tcctgctgca cctcgagctg
     9901 ttcttcccgt tcgcgtggcg cgccgggcgg ttcgcgctcc tgcgggacgc ctttctcgcc
     9961 ggcgccggtg tgtcgtccga ccccgtgggg ctcgagctgt gccgcaccgt cgcggcgctg
    10021 cgggcgctgg cccacgtgca cgtgcgctct tggcgcttct ggccgcacgc cgccctgttg
    10081 cggcgcgtca ccttgaacgg gggacgcgtc tgatgctgga ggtcatcttc tgggtcgcgc
    10141 tgctcgtcgt cgtctacgcg tatgccggct acggcgtcgt cgtctactgt ctcgtccgca
    10201 tcgcgcgcct gctgcggccg tccgtccctc accccgtcgt cgccgccgag gcgcccgaag
    10261 tgacgctggt cgtccccgcc tggaacgagg ccgcctggat cggggcgaag atcgccaact
    10321 gtctggagct cgactatccg cgggaccggc tgaagatcct cgtcgtgacc gacggctcga
    10381 ccgacgatac ggcggagatc gcgcgccggt ttgctggcgt cgaagtgctc gagcggcgcg
    10441 agcgggtcgg gaaggcggcg gcgatcaacc gcgcgatgga gtgggtgacc agccccatcg
    10501 ttgtgctgtc ggatgccaac accatgctca actgcgaggc cgtgcgcgag ctcgtgcgcc
    10561 attacgccga cgcgtccgtc ggcgcggtgg ccggagagaa gcgcatccgg cgccgcgaca
    10621 gcgcggcggc tgcaggcgag ggtttctact ggcggtacga atcgcggctc aagcggctcg
    10681 attccgagtt gtggacggtg gtgggggcgg ccggggagct gctgtcgtac cggcgtgagc
    10741 tgttcacgcc gctcgaggag gacgccgtgc aggacgattt cctcctctca ctgagaatcg
    10801 ccgccagggg ctaccgcgtg gtctacgagt cccgcgcgta cgcgaccgag gaggcttcca
    10861 tgacgatcgc cgaggagttg acgcgccgga tccgcatagc ggccggcggg tggcagtcga
    10921 tggtgcggtt gcggtcgctg ctcgatccgc gacgccatcc cctgttgacc ttccagtacg
    10981 tctcgcaccg cgtgctgcgc tggagcgtcg ttcccgtgct gctgctcctc ctcctgccgg
    11041 tgaacgccgt gctcgcgacc ggctccggcc cgctggcgcc gctgtaccgc gtgctgctcg
    11101 cgggccagct ggcgttctac gcgttcgcgc tggccggata cctgtgcgac cggcgggggc
    11161 tccggctccg tgcgctggtg cctccctatt acctggtcgt cacgaactac gcggtgctgc
    11221 gcgggctggg tcgcttcctg gcgggccgcc agccggcgac gtgggagcgg gcgcggcggg
    11281 cggcgttgct gctcctcgcc ctcggctggg cgtcgtcatt gccggcgcaa tcgcgcgacg
    11341 ccgcggtgca cgggcagttc acggtggatg ccgtccactt cgacaacctc ttcgaggcct
    11401 cggacggaca gccgcggcag acactgtggg gtcaacgcgt gcagggtcgc ctgtcggcgc
    11461 cgctcggcgc gcggggagtc gcgatctacg gggaagggga cgggatgcgg atccccgggt
    11521 tcgcgccggc ctacggcgcc ggcctcgggg tgcagcgcct gggcggcgtc acgaccttcg
    11581 atgccaaggt gagttacctg ggcaaccggc cggcggcgga aatcggatcg ggactcggac
    11641 ccgccaacct gctgcgcttc gacggtgaag gggcctatcg cccgttcagt gccgtacagc
    11701 tcgtcggccg cggacaggcg cgccgggagc agtacacctc ccaggtcatg cggaacggga
    11761 cggccttcga gttcggcccc ctggtccggt tccgggcgtt cgggtccggt ttctcccccg
    11821 aggcgggggc gatgttcggc cagcgggacg gccgcgaccc caacctggcg tatcgggagc
    11881 ggggatactt cgtcggcgcc agctcgctca ccttcggcta ccggctctac ctgagcgccc
    11941 gctaccgctg gcgcaccagg agctactcct ccgaggatcc gctcctcggc aacttcgggc
    12001 gggtggaccg gcggcagcag gtctcggtgg ccgtcgcggt cacgctcctg cccggactgt
    12061 cgtgggtcac ctactacgcg cacgagcgcg gggtctccac ccgccctggc gaaaacttca
    12121 ccacgggatt tctcagtgtg ggtctcgcca cgaagttctg aggtgcggcg cgcggtcaag
    12181 cgcctgctcg cccacagcca tccggcctac cgctgggcgc gccggctctc catgttcgtc
    12241 cgctacggct gcgggcggcc gcacgaggcc gacttcgccg gcttccgcca cttcgggcgg
    12301 cgcagcggcc tgttcctgga catcggcgcc aattccggcg agtcggtcct gtcgttccgg
    12361 ctgttcaacg cgaccacccg gattctttcg atcgagccca accgctatca cgaacccgac
    12421 ctgcggttcc tcaagcgatg ggtgcgccgc ttcgactacc gcctctgggc cgcgggcgac
    12481 gaaaacggct gggccacgct ctatgtgccg gtgtaccgga agctgccgct cacgggcgag
    12541 gcgtcgttcc accgggagca ggcgctcggc aactactgga tgcacgagca gctcggcggc
    12601 aacggggggg cgaaggaggg cgtgcggctc cgcgaggtgc cggtcgaggt gaaacgactc
    12661 gacgacctgc ggctgaagcc ggcgttcatc aagatggacg tgcaggggtt cgaggcgaag
    12721 gtcgtggctg gcctgcacga caccatctcc gccagccggc ccgtcctcct gatcgagcgc
    12781 tccggctgcg acgcggcgct cctcgcccgg ctcgcggacc tgggctacac cgcgttcctg
    12841 tacgtgcccg cgcgggacgc gttcgcgccc tggcgcgacg agccggccca gaacctgttc
    12901 ttcattcccg gccatacctc catccccatg acggcgccgc cgccgctcga gcagctggag
    12961 gtggtggggt gaccggcacg acgccacggg tcgtgggatc ccgtgtcgtc gtgtgcctga
    13021 tgggtctggt ggccgtggcg tcgcagcgcg cggcggcgca ggagctgcgc gtggccgtcg
    13081 gcgagcggga aatcgcacgc gtcgcggtct ccgagacccg gggctacccg gccctcccca
    13141 tcgacctgct cgagcccctc ggcgcgaagg tcgtggtcgg caaggacgtc gtccaggttc
    13201 ggctcttcgg cgacactctg cggttctatc cggggtacgc cgagttccag atcgacgtgc
    13261 ggacggaatc gctccaggcc ctgacctatc tgcaggacgg gacgctgtac gtcgcccagt
    13321 ggttcttcac ctactggctc cccaaccatt acccctggcg gctcacctac ggcgaccacg
    13381 tgctccggct caccgcgggc gcagtggcgg cgagcggcgg atccgacatc gcggcggcga
    13441 gggccgtccg cgagtctcct cccgcggccc ccgcggcgcg ggcggcgaga cgcgcgccgc
    13501 caccggcgga cacggctcgg tccgaggcgg tggcggattc cctggacgcc gagcggccgg
    13561 tgacccggga ccggacgtat gatcccgacg agccgctggg ggagatcctg ggcttcatcg
    13621 atgcgcgggt gtcgggcgtg tacgacagca acatcgacca ccgtaccgcg gccacccgct
    13681 cctacgggac cgtgggccgt ctgggggtgg ggatccagag cagtccggtg cggccgttcc
    13741 tgatggcgcg ctacgatttc gcgctctatc ggttcgcgaa cagcgaccag tggaagcgca
    13801 ccacgcacga cgtgagtgcg gacgtcgcac cgaccttcgg cgcggtacgg ctgcggctcg
    13861 gcgcggccct gcgcctgggc tcgtggaccg aggaccgcca gccggcgaac cagatcgtgg
    13921 tgcggccgca gatcgagttt cgcccgacgc cgatccacct gatcaacctg tacgtgatgc
    13981 agagcgcccg gcgcatcgac gtcgggacga ccaccctcag ggacacgttt cgtctggccg
    14041 ggctgggata ctacctctgg tggcacgccg gcggcttccg ggtggacgcg cgctacgagt
    14101 cggacaagtc cgagtatgta ccgagcagct acgacggctg gacgggatat gcctggacgc
    14161 gcatcccgtt caccgaatcg ttccgtgtca cactggagtc ggcgtacaac cggcgccgct
    14221 atgcccacag cttcgtggat ccggcgcaca ccatcatgcg gcgggatcgg cggtggaccc
    14281 tcacgacgtc cttcacgacc ctgctcccga cccccggctg ggaggtgagc ctcgtgtaca
    14341 cgttcgaaga cggccgttcg aacagcccct ccgcggtgta ccgggcgcac cgggtcgaga
    14401 ccatggtgcg gcgccgctgg tgacggcatc tttgctccca cgacatccca tcgtgaggag
    14461 cagccgtgcg ccaccgccgt ccgcggccct tcgccgcccg tgttcctgtg ctcaccttcc
    14521 tgctcgtcac cagcccggcc gccacgctgg tggctcagcg gcgcgcggcg ccgtcatcga
    14581 gcgggggggg cgccgccgcg atcgacaccg cgcgttacgc gcagctgcac ttccgacaca
    14641 tcggcccgga aggcaaccgg gtcagctcgg tggcgggggt ggccggcgac cccggcactt
    14701 actacgccgg cgcggcatcg ggcggcctgt ggaagagcgg ggacggcggc gtccactggg
    14761 acccggtgtt cgacgaccag ccggtctcgt ccatcggcgc gctggccgta gcgccgtcgg
    14821 atcccaacgt ggtctgggcc gggacgggcg agccgttcat ccggtcgaac gtctcggtcg
    14881 gctggggcgt ctacaagtcc accgacgccg gcaagcactg gacgaagatg gggctcgaca
    14941 gcaccggacg catttcgcgc atcgtcgtcc accccaccaa tccggacatc gtctacgtcg
    15001 ccgcgctggg ccatctgtac gggccgcagc cggagcgcgg catctaccgt accagcgacg
    15061 gcggcaggac ctgggagcgc gtgctgttcg tgaacgacag caccggcgcg tcggacctgg
    15121 tgatggatcc caacaacccg cgcatcctct tcgccgggtt ctggcaggtc gagctgcaca
    15181 cctggatccg ctcgagcggc ggcacgggca gcggcatctg gacctcgcgc gacggcggca
    15241 cgacctggac gcgcgtcacg gggagcgggc tgcccaccaa gccggtgggg aagatcggcc
    15301 tcgccatgac acgggcgaac tcgaaccgga tctatgcgct gatcgagacc ggggacggcg
    15361 tgcccgccgt gaacatcgcc cagcccgata ccgggcggct gttccgctcc gacgacggcg
    15421 gcgcgtcgtg gcagctcgtc agccaggacc gccaggtcgc cggccgcacg cactactaca
    15481 accgcatggc ggcggcgccc gacaacgaca acgaggcgta cttcctcacg gcctcgtggg
    15541 ccaagacgct ggacggcggg cagacgatca tcgacccgcc caacgcccag gtgccgggcg
    15601 gcgaccacca cgacatctgg atcgatccga ccaacggcaa ccgcatgatc gtgagccacg
    15661 acggcggcgt ctcgatcacg gccaatcgcg gccgcagctg gctgcgtgtg cagctcccga
    15721 ttgcgcaggt gtaccacgtg acgctggaca accgcgtccc ctactacgtg tacgggaacc
    15781 ggcaggacgg cccgtcgtcg cgcggcccga gcaacagccg caccggcggc gacattcccc
    15841 gcggaatgtg gggcacggtg ggcggagggg agagcggctg ggcgaccccc gatcccgtag
    15901 atcccgacat cgtgtggtcc agcgcctcgg ggttcggcag cgtgggcggg atcgtgtcgc
    15961 gctacgacgt gcgtaccggg atcgcgcgca gcaccgagat ctggccccag gcgacgatcg
    16021 ggtggccggc cggggatctc aagtaccgct tcgtgtggac cttcccgctc accatttcgc
    16081 cgcacgacca caacaccgtg tacgtcggta gccagcacgt gcacgtcacg tccgacggcg
    16141 gcgtgacgtg gaaggagatc agtcccgatc tcacgcgcaa cgacaagtcc cggcaggtgc
    16201 cctcgggcgg gctcacgccg gacaacatcg gcgtcgagta cgcgggcgtg gtgttcgcca
    16261 tcgccgagtc gccgttgaag cgcgggctca tctgggcggg gaccaacgac ggcaaggtcc
    16321 agctcacccg cgacggcggc ggccactggg ccgacgtcac cggcaacatc aaggggctgc
    16381 tcgactgggg gacgatctcg agcatcgagc cctcgcgcta cgacaccggc acggcctaca
    16441 tcgcggtgga cggccaccag gtgaacagcc gcgatccgtg gatctacagg accaccgact
    16501 acggggcgag ctggaccccg atcaccaccg gcttgccgaa gagcccgctg ggctatgtgc
    16561 acatcgtgaa ggaggacccg gtgcgccgcg gcctgctcta tgccggcgcg gagaacggcc
    16621 tgtacgtctc gttcgacgac ggcgcgcact ggcagccgct gcagaacaat ctgccgcacg
    16681 cgccggtgta cggcatcgcg gtgcagccgc agttccacga cctggccgtg gccacttacg
    16741 ggcgcggctt ctggatcctg gacgacatca cgccgctgcg cgcgctcacg cccgcggtca
    16801 cggccagtga tgcgtacctg ttcgccccac gatccgccta tcgcttccgc ggaatcgagg
    16861 gaccctacga ggcgtccgac gacccggtgg ccggcaccaa tcctccctac ggcgcctcgc
    16921 tcgactactg ggcgcgcacc gcgccgcgcg acagcgcgaa ggacagcgtc acgatccgga
    16981 tcgtggatgc gggcggcaag gtcgtgcgga ccatcacgca gcttccggcc gcgggggtga
    17041 accggctgtg gtgggacctg cgctacgagc cgacgcccga ggcgaagctg cggaccagcc
    17101 cgctctacgc cgcctggctc gcggtgaagc ccgaagggac gccggcgccc ggcatcggcc
    17161 gcttctcgct gctggccccg ccggggcggt acaccgtgcg tctcacggcc ggcgggaagt
    17221 cgttcgagca accgctcgat gtcctcaagg atcccaacac gggcgcgtcg gacgatgatc
    17281 tgcgggcgca aggcgtgatg atggacgcgg tcctcgccga tctggacagc accgtagcga
    17341 tgatcaaccg cgtcgagctg ctgcgcgggc agctcgcgtc gttgcgcgcc acgctggccg
    17401 gggactcgac cgcggccgac gtacgggccc gggccgacac gctcgaccga gcgctgcggg
    17461 cgttcgagga ggacttgttc caggtcaagg tgacggggcg ggggcaggac ctgctgcgct
    17521 ggccgatgaa gctggccgag cagctcatgt atctcgcgca atcgctcacc agctccgact
    17581 tcgcccccac cggcccgcag cgcgacgtgc aacaactgct ggccgggcag gtgcgggaga
    17641 gccggacgcg gctcgatcgg ctgatggcgg gcgacgtcgc gacgtttcgg cagttcctgc
    17701 ggtcgcggaa cctgccaaac ctgattccct agggaggctc tgcgtaaggg agggcggtcc
    17761 ttcgtaagcg gcgcgacatc gggggtgggg gcaaggacgc gaggacgcgg gggccgcacg
    17821 gcccgggcct tatcgctcat ggctggcaat gagatgcgaa gcgtcggccc gggtcgagcg
    17881 cccccgcgtc taacgcgtcc tagccccgac ccccgacgtc gcgcggattt gggcagaggt
    17941 gcctgagagc cttctgaaca gctcctgaca ttcccacggt gcctgaggtc tcgcggtggg
    18001 gctagactgc gacgaccact tttccggtac tcgaggaccg acatgagtgt cattccagtc
    18061 gtattgcgcc ggcgcatcgt cgcggtcgcg ccgctcgccg gtgcgctcgc gatcggcccc
    18121 gcgtcgctgc gcgggcagca agcggcgacg gtcgtgccgg gcgtcgtcgt gcacatggat
    18181 gccgcccccg ggcccgacac ggtcgtgttc cgccggacga tcacgcgcgg cggagtcgac
    18241 tcgatcaccg ggacccgcac ggtcgtgtcg cgcgtcgtgc caggtcaggg cggcgctcgg
    18301 ctgctcgaag tcgaacagcg cttccccggc ggcgggggcg agatcgtgga taccgcgctg
    18361 gccgacctcg gcacgctgcg cgcagtggcc catcgctccc atcagccgag gcgcaccatg
    18421 cgcttcgatt tcgtggatgg tgctgccgag gggtccctga gcgacagcgg tgcggtgcgc
    18481 caggagctcg gcgggccgat cttcgattcc aacgtgatcg agctggtggt ggcggcgctg
    18541 ccgctcgacg cgcggttcag cgccgagctc cccttcttca tttatgagcg cgggggccgg
    18601 gtgccgatgg cggtggcggt gcgggagcgg gcgcgcgtgg cgttccccat gctgggggag
    18661 cgcgaggcgt ggatcgtgac ggtgggggtg cccggggcgc cggcgacgat ctgggtggat
    18721 accgcgacgc gcgccgtgct gcgcacccgt tacgacatca cggcgcgagc catgagcctc
    18781 accgatgacc gtgtcacgcc gctgcacggg tgacggcagg tcacgctggc tgactgctgg
    18841 atcgactgag gggacacgac ggctacttgg cggcatcgct ggctgggcgt tcgtcctgga
    18901 ttccgtctca aggacgcaaa cggttcttga tggtgcgtcg gcagctcatg ggaatccgcg
    18961 atcgggcaga gcgattggcc cgcgccgact cggcgtattt tcgggccgcg gtcccaacgt
    19021 cccacgctac aggctcatcc gaacccacca tgctgcggcc agtagccgac tatctggggg
    19081 cgggagcagc cgatcgagct gtgcttgcgt ctcgggccat gccgcgagtc cggtgaggag
    19141 cacgaagtcc ggcatcccgt acagcgccgt ccagctcgtc acgagggtcg ccgcctcgcc
    19201 gtccatcgcc atgctgcggt agtcctgatt gggcgacccg atctgcagat agaataccca
    19261 gcgcaatccg ggacagtgct gcggtcgcgg gcaggcccgc gcggagtcgg ggagactgtc
    19321 gagcacggcg ttgatgattg tcgcgcctat gcgctgcatc gcgtcggcca tggcttgctc
    19381 gccgacaccc tcgccgctct ccagctgctt gttccgttcc cccagatact cccgcagccc
    19441 gtacgccatc ggtggaccgc tgatcaatcg cgcccaggcc tcgcgcgaga tgtagagaaa
    19501 ccccttcatg tgcagcaacg gttgcactgt cgcgccgggc gcccgccgcg tggcttc
//
LOCUS       DBNGZW010000262         7287 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1379899, whole genome shotgun sequence.
ACCESSION   DBNGZW010000262 DBNGZW010000000
VERSION     DBNGZW010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7287)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7287)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7287
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1379899"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..585
                     /locus_tag="AC16JC_06300"
     CDS             <1..585
                     /locus_tag="AC16JC_06300"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5164343.1"
                     /translation="REALAPSERERLRTLASDQSSRTDVDALVAFLGDPRTDIEGAIL
                     GVGVNPMYDEGPRRLDRLSAATVEATIRTNCTHALLLTSVLLRRLRERGGGVLVWIGS
                     QGAAAGLPGAGVYCATKAFLSGLARAAHNEYSGRGVRVHLAHPGLMRTPRTAAVADRF
                     AAVHGIAVGDVDEIAGRLVTLFLSADAACVEVNL"
     gene            582..1361
                     /locus_tag="AC16JC_06305"
     CDS             582..1361
                     /locus_tag="AC16JC_06305"
                     /inference="COORDINATES: protein motif:HMM:NF012360.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HIR5164344.1"
                     /translation="MIALDLLWWLGWAWLAAWAALPLLLLPWLGPVGGFVAWAVLAPW
                     SALVGMAGMHRLLPRSEPGRFRLFADSGAVRWALKGWAPSAYLAVFQPVFFMSEGFQR
                     LVLRAFGARLGVGALVTSRTVIREPHHVQIGAGSLLGEFVHLAPAYQPRPKLLVVADI
                     VIGDAVLIGAHSVLAPGASVGHRSILEHAVSIGAHVVIGDDVRIGGGSTLYTGVRVGN
                     RAVIGKACVIPAGTVIPAGAVIPDGSVLPAPRAGRRERSSA"
     gene            1358..3001
                     /locus_tag="AC16JC_06310"
     CDS             1358..3001
                     /locus_tag="AC16JC_06310"
                     /inference="COORDINATES: protein motif:HMM:NF015291.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HIR5164345.1"
                     /translation="MIASLLFALSYRRFLAACRDPFGAQARVLRRILRQAAHTRIGEA
                     HEFSRIARLRDDREVIRTFQAAVPIRAHAAMREDLEAVYAGDWRRLCPSRPLFFAMTA
                     GSTGRYKYIPFTREFRHELGVGSRIFYGALEAGSPGLRGRKAQFLVGSAEGGRTPDGI
                     PQGFASGFNYRNLPHLVRDRFILPYWIFTLEDVEDRCYAAGRLLAGNRDLGALCAISP
                     VNLMNVRQALERNAERLFRDMEDGTLTLRGAAVVPGEYHGRPDPVLAEALRDAWMRDG
                     TLPNRLLFPSLELLVCWQGGNMSYYLDELDRHFGLSRHFEFPISASEGIFAIPHRRDR
                     AGGILAVTSHFLEFLPEDEASDSPEPLTADRLRPGAEYRLVVTNSGGLYRYDMEDIVR
                     VTSQYRATPVIEFVSKKDRQVSVSNERITELDVTVAMQAACRAAGRWFPEFLFVPCTD
                     RRYRVVIDGTAAGTEQDGFAAELEGQLRRAARGYDFEREDGLLEPLELIVTVPGELRD
                     WLERRQGGPRLANAQIKPQHLTNQFDAHTAFHAVATRAA"
     gene            2991..4016
                     /locus_tag="AC16JC_06315"
     CDS             2991..4016
                     /locus_tag="AC16JC_06315"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF020130.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="HIR5164346.1"
                     /translation="MQLSAPNVRIRDVESCDFGLLPRYDTATLLRAALPDRADDYLAS
                     AVSRKLLAELGVEQRYLTHLPGSPPDPARLTALDLACSAVTRLRARRAQELEHLDALI
                     FVSTSNPNPVNSQAALLAGALGLRASCLDLKAGCSGGVLGLMHAATLIHGGCERVLVV
                     MAENLSQLTPPDDPRMLLTVGDGAACVLVERHPGPGFLSMIHGSAPEWAGTMAIPEPF
                     PPRAPDVRYQFEFREAPRAAEFLRARWRSLFHDSLAAAGRSVDDLAHCFFHQTHAAQV
                     EGLRADLGLSPERLADVVRCHGNMGSPTFAVAMSRRFGRLRPGETYLMQAVGGGISWC
                     AIVAEHR"
     gene            4024..6300
                     /locus_tag="AC16JC_06320"
     CDS             4024..6300
                     /locus_tag="AC16JC_06320"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR5164347.1"
                     /translation="MNLARIIVRRRGWIALAWVAAAAALLPAARHAASVLDVSARVEG
                     SESAAVEQLLGGPLASAYARYVVLVIGGVPSPATPAGAAVLREITDSLLQARQIAAGG
                     IFTYLDQPDTLFLAPRGRGTFVLVGLAADSGAPDRLIPPLRALTQSLAPRLRERFPDL
                     TLRWTGETALNVDLRRTSSVDVGAAERRALPLTAVFLLIAFGAVAASAVPVVAGGLAI
                     ALALGAAVLLARVWPLSILLQSVVTMLGLGLGIDYALLTVSRFREGRDAGEGPELAAE
                     HAARHAGHTILLSATAVAIGFVVLVTVRLNEIRAIAVGGLVVVVTSALLAATLLPGVL
                     AWAGPKIDWGRLRRRVPGRASGQRWRRLGRLVTAHPWKALGLGALPLVLLAAQATRLR
                     TGLPRGDWLPPAMESARALEDLRAMGRSNVVQTIRVVLVLPQGSSVRRPEGWNALARM
                     ADSLGADPRVERIRSVVGIARSAGLGRALTFLPARIKQGLVSSDGRYALLELLPKESV
                     GAEEVVAYVRELRRRGAAASGVAGARLPVGGLPAFNTDYSDASAGHLPEVVVLVIAGT
                     LLTLFAGARSILIPVKAVLLNLLSVAAAFGALTLVFQEGHGLRLFGITEPLGAVFSSL
                     PVIVFCIVFGLSMDYEVFLVTRVMEARRSGMSDADAIVEALGTTGQVITSAAAIMLVV
                     FGAFTLGSFQFTKMLGFTLAVAVLLDATLVRMVVGPALLRLAGRWNWWPGRVVAVPGG
                     MMPSAGPGRHPRAPRDVS"
     gene            complement(6303..7115)
                     /locus_tag="AC16JC_06325"
     CDS             complement(6303..7115)
                     /locus_tag="AC16JC_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018129765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HIR5164348.1"
                     /translation="MPDAHPPREIAANVETLGVAKARMGALTVLVLAVLAGAFIALGA
                     LFFIVVVTDSGLGFGVTRLLGGLAFCLGLVLVLVAGAELFTGNNLLAMAWASGLIGSR
                     DVLRNWLLVYAGNVLGCLGTVVLVLWGGIAGLDAGAVGETAIHVAGAKAALSFEGAFA
                     RGILCNALVCLAVWLAMGGRSVADKIVAVLFPITAFVAIGFEHSIANWFFLPYGLALD
                     HQHAVPVAGVARNLVAVTAGNIAGGTLLVAGVYWVAYLRGGRTPAPGKPARS"
BASE COUNT          939 a         2529 c         2664 g         1155 t
ORIGIN      
        1 cgggaggcgt tggcgccgtc cgagcgggag cgtctccgca ccctggcgag cgaccagagc
       61 agccgcaccg acgtggacgc gctcgtcgcc ttcctcgggg atccccgcac cgacatcgag
      121 ggcgccatcc tgggcgtcgg cgtgaatccc atgtacgacg agggaccgag acggctcgac
      181 cgcctcagtg ccgccaccgt ggaagcgacg atccggacga actgcacgca tgccctgtta
      241 ctcacgtcgg tgctgctgcg gcggctgcgc gagcgtggcg gcggcgtgct ggtctggatc
      301 ggctcacagg gtgccgcggc gggactgccg ggggccggcg tgtactgcgc cacgaaagcg
      361 ttcctgtcgg gcctcgcgcg cgcggcgcac aacgaataca gcggtcgcgg cgttcgcgtc
      421 cacctcgcgc acccgggtct gatgcgcacg ccccggaccg ccgcggtcgc ggaccgcttc
      481 gccgcggtgc acggcatcgc ggtcggtgac gtggacgaaa tcgccggccg gctcgtcact
      541 ctgtttctct cggcagacgc cgcgtgcgtg gaggtgaacc tgtgatcgcc ctcgacctgt
      601 tgtggtggct ggggtgggcg tggctcgccg cgtgggccgc gttgccgctg ctgctcctgc
      661 cgtggttggg tcccgtcggc gggttcgtgg cgtgggccgt gctcgcaccc tggagcgcac
      721 tggtcggcat ggccggcatg caccgcctcc ttccccgcag cgagccggga cgcttccggc
      781 tcttcgcgga ctccggggcc gtccgctggg cgctcaaggg gtgggccccg tcggcgtacc
      841 tcgccgtgtt ccagcccgtc ttcttcatga gtgaggggtt ccagcgcctc gtgctgcgtg
      901 ccttcggggc gcggctcggg gtgggcgcgc tggtcacctc gcgcaccgtg atccgcgagc
      961 cacatcacgt ccagatcgga gcggggagcc tcctgggtga gttcgtgcat ctggcgcccg
     1021 cctaccagcc gcgtccgaag ctcctagtcg tcgcagacat cgtgatcggc gatgcagtgc
     1081 tgatcggcgc gcacagcgtg ctcgctcccg gtgcttcggt ggggcaccgc agcatcctgg
     1141 agcacgccgt gtcgatcggc gcccacgtcg tgatcggcga cgacgttcgc atcggcggcg
     1201 ggagcacgct ctacaccggg gtacgcgtcg ggaaccgggc cgtgatcggg aaggcgtgcg
     1261 tcatccccgc ggggacggtg atccccgcgg gggcggtgat cccggatggc tccgtgctgc
     1321 cggcgcctcg cgccgggcgg cgtgaacgga gcagcgcatg atcgcgagcc tgttgttcgc
     1381 cctgagctac cggcgctttc tcgccgcgtg ccgcgatccc ttcggggccc aggcgcgcgt
     1441 gctgcgccgc atcttgcggc aggcggcgca cacgcgcatc ggggaggcgc acgagttctc
     1501 ccgcatcgcc cggctgcggg acgaccggga ggtgatccgg accttccaag ccgcggtgcc
     1561 gatacgcgcg catgcggcta tgcgtgaaga cctggaggcc gtgtacgcgg gcgactggcg
     1621 tcgcctgtgc cccagccggc cgctcttctt cgcgatgacc gcgggctcca ccggccgcta
     1681 caagtacatc ccgttcaccc gcgaattccg ccacgagctc ggcgtggggt cgcggatctt
     1741 ctacggagcg ctcgaagcgg ggtccccagg cctccgcggc cgcaaggcgc agtttctcgt
     1801 cgggtcggcg gagggcggca ggacgcccga cggcatcccg caggggttcg cgtcggggtt
     1861 caattatcgg aacctccccc atctggtgcg cgaccggttc atcctgccgt actggatctt
     1921 caccctggag gatgtcgagg accggtgcta cgccgcgggg cggctcctcg ccgggaaccg
     1981 cgacctcggc gcgctgtgcg ccatctcgcc cgtgaacctg atgaacgtgc gccaggcgct
     2041 cgagcggaac gccgagcggc tcttccgcga catggaagac gggacgctca cgctgcgcgg
     2101 cgcagcggtg gtgccggggg agtatcacgg gcgtcccgac ccggtgcttg ccgaggcact
     2161 ccgggacgcg tggatgcggg acgggaccct gccgaatcga ttgctctttc cctcgctcga
     2221 gctgctggtc tgctggcagg gcggcaacat gagctattac ctggacgagc tcgaccggca
     2281 cttcggcctg agccgtcact tcgagttccc gatttcggcg agcgagggga tcttcgccat
     2341 tccgcaccgc cgcgaccgtg ccggcggtat cctcgccgtc acctcgcatt tcctcgagtt
     2401 ccttcccgag gacgaggcca gcgactcgcc cgaaccgctc accgcggatc ggctccggcc
     2461 gggggccgag taccggctgg tcgtgaccaa ctcgggcggc ctctatcgct acgacatgga
     2521 agacatcgta cgggtgacct cgcagtaccg ggcgacgccg gtgatcgagt tcgtctcgaa
     2581 gaaggaccgc caggtatcgg tgtcgaacga gcggatcacc gagctcgacg tgacggtcgc
     2641 gatgcaggcc gcctgccggg cggccggacg atggttcccg gagttcctgt tcgtgccgtg
     2701 caccgaccgg cgctatcgcg tggtgatcga cggcaccgcg gccggaacgg agcaggacgg
     2761 cttcgccgca gagctcgaag gccagctgcg tcgagccgcg cgcggctacg acttcgagcg
     2821 cgaggacggc ctgctcgagc cgctcgagct catcgtgacg gtgccggggg agctgcgcga
     2881 ctggctcgaa cggcgccagg gagggccccg gctcgccaac gcgcagatca agccgcagca
     2941 cctcacgaat cagttcgacg cgcatacggc ctttcacgcg gtggcgaccc gtgcagctta
     3001 gcgcgccgaa cgtccgcatc cgggacgtcg agtcctgcga cttcgggctc ctcccgcggt
     3061 acgacacggc gacgttgtta cgcgcggcgc ttccggaccg ggcagacgac tacctcgcgt
     3121 cggccgtatc gcggaagctc ctcgccgagc tgggcgtcga gcagcgctac ctcacgcacc
     3181 tgcccggatc tccgcccgat ccggctcggc tcaccgcgct cgacctcgcg tgctccgcgg
     3241 tgacgcggtt gcgggcccgg cgcgcccagg agctcgagca cctggacgcg ctgatcttcg
     3301 tgagcacgtc gaaccccaac ccggtgaaca gccaggcggc gctgctggcc ggggcgctcg
     3361 gcctccgggc gtcctgtctg gatctcaagg cggggtgcag cggcggcgtg ctgggcctca
     3421 tgcacgcggc gacgctgatt cacggtggct gcgagcgggt cctcgtcgtg atggcggaga
     3481 acctgtcgca actcaccccc cccgacgacc cgcgcatgct gctcacggtg ggtgatgggg
     3541 cggcgtgcgt gctggtcgag cggcacccgg gaccgggctt cctgagcatg atccacgggt
     3601 cggcgcccga gtgggcgggg acgatggcga ttcccgagcc gttcccgccg cgggcgcccg
     3661 atgtgcgcta ccagttcgag ttccgtgagg cgccgcgcgc ggccgaattc ctgcgcgcgc
     3721 gctggcgctc gctgtttcac gacagcctcg cggccgccgg tcgatccgtc gacgacctgg
     3781 cgcactgctt cttccatcaa acgcatgcgg cacaggtcga ggggctgcgg gccgatctcg
     3841 gtctctcacc ggagcgcctg gcggacgtag tgcgctgtca tgggaacatg gggtcgccga
     3901 cgtttgccgt ggcgatgtcg cgccgattcg gccggctgcg gccgggcgag acctacctga
     3961 tgcaggcggt gggcggcggc atcagctggt gcgcgatcgt cgccgagcat cggtgaggca
     4021 gccatgaacc tggcgcgcat catcgtccgc cggcgcggct ggatcgcgct ggcctgggtc
     4081 gcggcggccg cggcactgct cccggccgca cggcacgcgg cgagcgtgct cgacgtgagc
     4141 gcacgcgtgg agggaagcga atcggctgcg gtggaacagt tgctcggcgg cccgctggcg
     4201 tccgcctatg cccgctacgt ggtgctcgtg atcgggggcg tgccgtcgcc cgcgacgccc
     4261 gcgggggcgg ccgtcctgcg ggaaatcacc gactcgttgc tccaggcgcg acagatcgcc
     4321 gccggaggga tcttcaccta cctcgatcaa ccggacaccc tgttcctcgc accgcgcggg
     4381 cgcggcacgt tcgtgctcgt cggtctcgcc gcggacagcg gtgcaccgga ccggctgatc
     4441 ccgccgttgc gcgccttgac gcagtcgctc gcaccgcggc tgcgggagcg gtttcccgat
     4501 ctgacgctcc gctggaccgg cgagacggcg ctcaacgtgg acctgcgacg cacgagcagc
     4561 gtcgacgtcg gcgcggccga gcgccgggcg ctgccgctga ccgcggtgtt tctcctgatc
     4621 gccttcggtg cggtggcggc gagcgcggtt cccgtcgtcg ccggcgggct ggcgatcgcc
     4681 ctggcgctcg gggccgcggt gctcctcgcc cgcgtgtggc cgctctcgat tctgctgcag
     4741 agcgtcgtga ccatgctcgg cctcgggctg ggcatcgact acgcgctgct tacggtgagc
     4801 cgcttccgtg aagggcgcga cgcaggggag ggaccggagc tggcggcgga gcatgcggcg
     4861 cgacacgccg ggcacacgat tctgttgtcc gccaccgccg tcgccatcgg gttcgtcgtg
     4921 ctggtcaccg tccgtttgaa cgagatccgg gccatcgccg tgggagggct cgtcgtcgtg
     4981 gtgacatccg ccctgctcgc tgcgaccctc ctgccgggcg tgctcgcgtg ggcgggcccg
     5041 aagatcgact gggggcggct gcgccgccgc gttccggggc gcgcgtccgg tcagcgctgg
     5101 cgccgcctcg gccggctcgt cacggcacat ccgtggaagg cgctggggct gggcgcactg
     5161 ccgctcgttc tgctggcggc gcaggccacg cggttgcgta ccggtctccc gcgcggcgat
     5221 tggctcccac cggcgatgga gtcggcgcgc gcgctggagg acctgcgggc gatggggcgg
     5281 agcaacgtcg tccagacgat ccgcgtggtg ctggtattgc cccagggcag ctcggtgcgt
     5341 cgccccgaag gctggaacgc cctcgcccgg atggccgatt cgctcggggc ggacccccgg
     5401 gtcgaacgga tccgctcggt cgtgggtatc gcccgctcgg cgggactggg gcgcgccctc
     5461 accttccttc cggcccgcat caagcaggga ctcgtcagca gcgacgggcg ctacgcgctg
     5521 ctcgagttgt tgccgaagga gtccgtcggc gcggaagagg tggtggccta cgtgcgcgag
     5581 ctgcggcgcc gcggggccgc cgcgtcgggc gtcgcgggcg cccggttgcc cgtcggtggg
     5641 ctcccggcgt tcaataccga ctacagcgac gcatcggcgg gccacctccc ggaggtcgtg
     5701 gtgctggtga tcgccgggac gctgcttacg ctcttcgccg gcgcccgctc gatcctgatt
     5761 ccggtgaagg ccgtgctcct taatctcctc tcggtcgcgg cggcgttcgg agccctgacg
     5821 ctggttttcc aggaggggca cggcctgcgg ctgttcggga tcaccgagcc gctgggcgcg
     5881 gtcttctcgt cgttgccggt catcgtgttc tgtatcgtct tcgggctgag tatggactac
     5941 gaggtgtttc tggtgacgcg ggtgatggag gcgcggcgct cggggatgag cgacgccgat
     6001 gcgatcgtcg aggcgctcgg caccacgggc caggtcatca ccagcgccgc cgccatcatg
     6061 ctggtcgtgt tcggcgcgtt cacgctgggg tcatttcagt tcaccaagat gctcgggttc
     6121 actctcgccg tagcggtgct gctggacgcg acgctggtcc gcatggtggt cggacccgcg
     6181 ctgctccggc tcgcgggacg ctggaactgg tggccgggtc gcgtcgtggc ggtgccaggt
     6241 gggatgatgc cgtcagccgg gccggggcgt cacccgcgtg cgccgcgcga cgtgtcgtga
     6301 gcttacgatc gagccggctt tcccggcgcc ggggtgcgcc cccctcgcag gtacgccacc
     6361 cagtacaccc ccgcgacgag cagcgtcccg ccggcgatgt tgcccgccgt gaccgcgacg
     6421 aggttccggg cgactccggc gaccggaacg gcgtgctggt gatcgagcgc cagcccgtag
     6481 gggagaaaga accagttggc gatcgagtgc tcgaagccga tcgccacgaa ggcggtgatg
     6541 gggaagagga ccgccacgat cttgtcggcc acgctgcgcc cgcccatcgc gagccacacc
     6601 gcgagacaca cgagcgcatt gcacagaatg ccacgggcga acgcgccctc gaatgagagc
     6661 gccgccttgg cgccggcgac gtggatcgcc gtctcgccta cggcgcccgc gtcgagaccg
     6721 gcgatgcccc cccacaggac cagcaccacg gtgcccagac acccgagcac gttgccggcg
     6781 tagacaagga gccagttccg cagcacgtcg cggctgccga tcaatccgct cgcccacgcc
     6841 atggcgagga ggttgttccc ggtgaacagc tcggcgcccg cgaccagcac cagcacgagg
     6901 ccgaggcaga aggccaggcc gcccagcagc cgggtcacgc cgaagcccaa accggaatcg
     6961 gtcacgacga cgatgaagaa cagggcgccg agggcgatga aggcacccgc gaggacggcg
     7021 agcacgagta cagtcagggc gcccatccgc gctttcgcca cgccgagggt ctcgacgttg
     7081 gcggcgatct cgcggggcgg gtgggcgtcg ggcacggggc taaggtgtac cgcgggccgg
     7141 gtccgatcaa gatcaccccg cccacattcc cgtcctgggc catgctccgg gcgggcaggc
     7201 cgctcagttc gctgctgtga tcttggcgag cagccagccg tgcggatcga tcaggaccct
     7261 ggtgggctcg gaggccgacc agactcg
//
LOCUS       DBNGZW010000263         2302 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_160553, whole genome shotgun sequence.
ACCESSION   DBNGZW010000263 DBNGZW010000000
VERSION     DBNGZW010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_160553"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..580
                     /locus_tag="AC16JC_06330"
     CDS             <1..580
                     /locus_tag="AC16JC_06330"
                     /inference="COORDINATES: protein motif:HMM:NF020698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5164349.1"
                     /translation="SCTTGGGTASLITVTAKDAFGNVISGATVTLGSTGTSNNFTPAG
                     NTNSSGVATSTFSSTKAEPKTVSAAINGVTITQTASVTVDPGVATQLTITTTVSNAKA
                     NTSISPPVQVTALDQFGNVATGFTGQVTMAFGTDPSGGSAILTYTPANALLGVAVFSD
                     IQIDTPGNGYTLVASASGLSSSAPSNSFNITP"
     gene            631..>2302
                     /locus_tag="AC16JC_06335"
     CDS             631..>2302
                     /locus_tag="AC16JC_06335"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5164350.1"
                     /translation="MICPRCSVGEISEATQECLVCGFSRTGGVLVETSTEWNRVEDPL
                     LPALERQFAVEGVLRRGSTSRIYLARDQVTGRLCSLKVLPLGEDVPEGLIERFRKDAQ
                     RAATLDHPHLVSLLSFGATDEAIWYAMEYVQGRSLGDLLRSGDRMDLKACLRLVEQIA
                     SALEYGHRRGITHGNLKPANVLVDPEGWARVADLGVSAAFGPLPRRAPELAIEENYGH
                     TAPEQFGPEGRLSPAADQYALAVLAFHCLARRPPIVGDTLEEITRHHVEGPVPLIAES
                     RPDLPAYVSSALARAMSRAPADRFPGVLDFVTALSGVEVVQATEEKATVDTAAEAQPS
                     PPEPAPAASAESTVVPSTSAAPASTSEPEAERTPEPEPELVAAMSETAAATSTASPEP
                     AVAEPSAPGGTEPPGPPLLLVERPHVHHDEDDEDEDAKPKEKEPVLLWDDQGHDAAVA
                     AAVATAATAENTPANESRRALEKLWSQLRPRLQQLVTLSRQLAARWATLPRNLRYGSS
                     AGAAVLVLAVVLWGVAHAGRAERRPPTPINEPRPTGVLPPSQTASPSAP"
BASE COUNT          385 a          832 c          766 g          319 t
ORIGIN      
        1 gtcgtgtact acgggcggag gaacggcctc attgatcaca gtgaccgcca aggatgcgtt
       61 cggcaacgtc atttccggcg cgaccgtgac tctcggttcc acgggcacga gcaacaactt
      121 caccccagca gggaacacca attccagcgg cgtagcgacc tcaacgttca gctcgaccaa
      181 ggccgaaccc aagacggtgt cggccgcaat caatggagtg accatcacgc agactgcgtc
      241 cgtcacggtt gaccctggcg tggcgaccca gttgacgatc acgaccacgg tcagcaacgc
      301 gaaggcgaat acgagcattt cgccgcccgt gcaggtgacg gccctggacc aattcggcaa
      361 cgtggccacc ggcttcactg gacaggtgac gatggcgttc ggtacggacc ccagtggcgg
      421 tagtgcgata ttgacctaca ctcccgcaaa cgcgttgctg ggggttgcgg tgttcagcga
      481 tattcagatc gatacacccg gtaatgggta tactttggtg gcgagcgcga gcggcctcag
      541 cagcagcgcg cccagcaaca gtttcaacat cacgccgtaa ccgggagtga cgacttcggc
      601 cgtgctcact cgcatccttt accgcccacg atgatctgcc cccgctgttc ggtcggcgag
      661 atctccgaag ccacccagga atgcctggtc tgtggcttct cgcgcacggg tggcgtcctc
      721 gtcgagacga gcaccgaatg gaaccgcgtc gaggatccgc tcctccccgc cctggaacgc
      781 cagttcgcgg tcgaaggcgt gctgcgccgc ggctccacct cccgcatcta cctcgcacgc
      841 gaccaggtca ccggccgcct ctgctcgctc aaggtcctgc cgctcggtga ggacgtgccc
      901 gaaggactga tcgagcgctt ccgcaaggac gcgcagcgcg ccgccacgct cgatcacccg
      961 cacctcgtct ccctgctcag cttcggcgcc accgacgagg ccatctggta cgccatggag
     1021 tacgtgcagg gccgctcgct cggcgacctg ctgcgcagcg gcgatcgcat ggacctgaag
     1081 gcgtgcctgc ggctcgtcga gcagatcgcc agcgcgctcg agtacgggca ccgccgcggc
     1141 atcacccacg gcaacctgaa acccgccaac gtgctggtgg accccgaagg ctgggcgcgc
     1201 gtcgccgatc tcggcgtgag cgccgccttc ggcccgcttc cccggcgcgc gcccgaactg
     1261 gcgatcgagg agaactacgg gcacaccgcc ccggagcagt tcgggccgga aggccggctg
     1321 agccccgccg ccgaccagta cgcgctggcc gtgctcgcct tccattgtct ggcacggcgt
     1381 ccgccgatcg tgggggatac gctcgaggag atcacgcggc accatgtcga agggccggtg
     1441 ccgctgatcg ccgagtcgcg ccccgacctg ccggcgtacg tatcgagcgc gctggcgcgc
     1501 gccatgagtc gcgccccggc cgatcgcttc cccggagtgc tcgacttcgt caccgccttg
     1561 tcgggagtgg aggtagttca ggcgacggag gagaaggcga cggtggatac ggcggcggag
     1621 gcgcagccgt cgccgcccga gccggcgcca gccgccagcg ctgaatcgac cgtcgtcccc
     1681 tcgaccagcg ccgcacccgc gtccacgtcc gagccggagg ccgagcgcac gccggagcct
     1741 gagcccgagc tggtcgccgc catgagcgaa accgcggcgg cgacctcgac cgccagcccc
     1801 gagcctgcgg tcgccgaacc gtcggccccg ggcggcacag agccgccggg cccgccgctg
     1861 ctgctggtgg agcgccccca tgtccaccac gacgaagacg acgaggacga ggacgcgaag
     1921 ccgaaggaga aggagccggt gctgctctgg gacgaccagg gccacgacgc cgcggtcgcg
     1981 gcggcggtag cgacggcagc gacggcggag aacactcccg cgaacgaaag caggcgcgcg
     2041 ctggagaaac tgtggtcgca gctgcggccg cgactgcagc agctcgtgac gctgtcccgg
     2101 cagctggccg cccgctgggc gaccctgccg cgcaacctac gctacggctc atcggccggc
     2161 gccgccgtac tggtgctcgc cgtcgtgttg tggggcgtgg cgcatgcggg aagggccgag
     2221 aggcgccccc ccacccccat caacgagcca cggcccaccg gcgtgctgcc cccgtcgcag
     2281 accgcgagtc cgtccgcacc gg
//
LOCUS       DBNGZW010000264         3661 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_698898, whole genome shotgun sequence.
ACCESSION   DBNGZW010000264 DBNGZW010000000
VERSION     DBNGZW010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_698898"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="AC16JC_06340"
     CDS             complement(<1..814)
                     /locus_tag="AC16JC_06340"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5164351.1"
                     /translation="MTPARIVRTYLLITGLFNFAASLIWGVDTLFKLHAGLDIFQVML
                     TNAAFTFGMVVFEIPTGVVADTLGRRVSFILCVVTLLLSTLLYVATAWFHWGFWPFMG
                     TSVLLGLGYTFYTGAVDAWLVDALKATGFAQPMEGVFAKGQMMFGAAMLAGTLGGGLL
                     GQIDLAVPYLVRSATFLPLLGVAWFAMQEIGFTPRALEWRRVPVEMRRVFVEGLDYGL
                     HHRVVRPVMLASLVGMSFMIFGFYSWQRYFLDLLGRDLVWVSGLIAALLSLAM"
     gene            complement(811..1689)
                     /locus_tag="AC16JC_06345"
     CDS             complement(811..1689)
                     /locus_tag="AC16JC_06345"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5164352.1"
                     /translation="MSPRLRVVASAVLFSTGGAAIKLCDFGSWQLAASRALVAAVAML
                     VLIPEARSGWTWRSPVVGIAYAGAGLFFILANKLTTAASAIFIQATHPLFILVLAPVL
                     LHEHVSRRDLEFMVLLAVGMALLLSEPGRRFATAPNPRLGNMLAAGCAVSWALTVMGY
                     RWVARQARGGHGAVVAAAVSGNALVFLVALPPALPLVAGGAGDWLVVLYLGVFQLALA
                     YVFLARAVAQVRALEVALVGLVEPVLSSVWAWLVHGETPGPRVVLGGAVILAATGWHA
                     WREPPPNVVAAGAIEG"
     gene            complement(1686..2540)
                     /locus_tag="AC16JC_06350"
     CDS             complement(1686..2540)
                     /locus_tag="AC16JC_06350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017288944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR5164353.1"
                     /translation="MLALALFALASCSHRDRVVVGAKNFTESDLLAEIAAQQIERRTG
                     LSVERKLHLGGTFVCHEAILAGKIDVYVEYTGTAYTAVLKHAPGPSADSIARAAASEY
                     RSRWGLVWLPPFGFNNTFAVAVRGADAQREGLHAISDLARVAPRWKAGFGYEFLNRED
                     GFPGLAKVYGLRFAAPPTAMDLGLTYRALADGQIDVIAGNSTDGQLDALHLVALADDR
                     HYFPPYEAAPVVRGELLERHPAVGRALAELAGKIDDATMRRLNAQADVEHKDLRAIAH
                     DWLEQTLP"
     gene            complement(2639..3271)
                     /locus_tag="AC16JC_06355"
     CDS             complement(2639..3271)
                     /locus_tag="AC16JC_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015189152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5164354.1"
                     /translation="MSLLRFYLDNSREVLGLLGQHVLLVIVSTVIAVAIGLPLGILLT
                     RRPAWRRPVLGLANIFQTIPSLALFGFLIPLPLLGGIGARTAIVALVVYALLPIVRNT
                     YTGIAGVDPAAREAGRGMGMTDGELLRLVELPLAASVILAGIRVATVVGVGTATIAAA
                     IGAGGLGVYIFRGVAVVDDRLILAGALPAALLALAADGLLGLLERRFAWR"
     gene            complement(3268..>3661)
                     /locus_tag="AC16JC_06360"
     CDS             complement(3268..>3661)
                     /locus_tag="AC16JC_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017742367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5164355.1"
                     /translation="IDARVRELLALVGLAPAQFAGRRPHELSGGQRQRVGVARALAVD
                     PPLLLLDEPFGALDPITRAELQHEFKGLEGRLGKAMVFVTHDVREGLLLGTRIGLMQG
                     GRMVFLGSTEQFRQSAHPEVRAFLEAAA"
BASE COUNT          656 a         1369 c         1208 g          428 t
ORIGIN      
        1 tcatggcgag cgacagcagc gcggcgatca ggcccgagac ccagaccaga tcccggccga
       61 gcaggtcgag gaagtagcgc tgccagctgt agaagccgaa gatcatgaag ctcatgccca
      121 ccagcgacgc gagcatcacc gggcggacca cccggtggtg cagcccgtag tccagcccct
      181 ccacgaacac gcggcgcatc tcgacgggga cccgccgcca ttcgagcgcg cggggcgtga
      241 acccgatctc ctgcatggcg aaccaggcga cgccgagcag cggcaggaac gtggccgagc
      301 ggacgagata ggggacggcg aggtcgatct ggcccagcaa gcccccaccc agggtgcccg
      361 cgagcatcgc cgcgccgaac atcatctggc ccttggcgaa cacgccctcc atcggctggg
      421 cgaatccggt cgccttgagc gcgtccacca gccaggcgtc caccgctccc gtgtagaacg
      481 tgtacccgag gccgagcagt accgacgtac ccatgaacgg ccagaaaccc cagtggaacc
      541 acgcggtcgc gacgtagagc agcgtcgaaa ggagcagcgt gaccacgcag aggatgaacg
      601 acacccgccg ccccagcgta tccgcgacca cgccggtcgg gatctcgaac accaccatcc
      661 cgaacgtgaa cgccgcgttg gtcagcatca cctggaagat gtcgagcccg gcgtggagct
      721 tgaacagcgt gtccacgccc cagatcagcg aggccgcgaa gttgaacaac cccgtgatga
      781 ggaggtaggt gcggacgatg cgggcgggcg tcatccttcg atcgcccccg ccgccacgac
      841 gttcggcggc ggctcgcgcc acgcgtgcca cccggtggcg gcgaggatca ccgcgccgcc
      901 caggaccacg cgggggcccg gtgtctcacc gtgcaccagc cacgcccaca ccgacgacag
      961 caccggctcc accagaccga ccagcgcgac ctcgagcgcc cgcacctgcg cgactgcgcg
     1021 cgccagaaac acgtacgcga gcgcgagctg gaacacgccg agatagagca cgacgagcca
     1081 atcgcccgct cccccggcga cgagcggcag tgccggaggc aacgcgacca gaaacacgag
     1141 cgcgttcccg gaaaccgccg ccgccacgac cgccccatgc ccgccgcgcg cctgccgcgc
     1201 cacccaccgg tatcccatga cggtgagcgc ccagctcacc gcacagcccg ccgccagcat
     1261 gttcccgagc ctcgggttcg gggccgtcgc gaatcgccgc cccggctcgg agagcagcaa
     1321 cgccatcccc accgccagaa gcaccatgaa ctcgaggtcg cgccggctca cgtgctcgtg
     1381 cagcagcacc ggcgcgagga ccaggatgaa caacggatgc gtcgcctgga tgaagatggc
     1441 gctggccgcg gtggtgagct tgttcgccag aatgaagaag agacccgcgc cggcgtacgc
     1501 gatccccacc accggcgagc gccaggtcca gccgctccgc gcctcgggga tgagcaccag
     1561 catcgcgacc gccgcgacca gtgcccggct cgccgcgagc tgccaggatc cgaagtcgca
     1621 cagcttgatg gcggccccgc cggtcgagaa cagcaccgcc gaggcgacga cccgcagccg
     1681 cgggctcacg gcagcgtctg ctccagccag tcgtgcgcga tcgcacgcag atccttgtgc
     1741 tccacatccg cctgggcgtt gaggcgccgc atggtcgcgt cgtcgatctt gcccgccagc
     1801 tcggccagcg cgcgacccac ggccggatgc cgctcgagca actcgccgcg gaccaccggc
     1861 gcggcctcgt agggcgggaa gtagtggcgg tcgtccgcca gtgccacgag atgcagcgca
     1921 tcgagctggc cgtcggtcga attgccggcg atcacgtcga tctgcccgtc ggccaacgcc
     1981 cggtaggtga gtcccaggtc catcgccgtc ggcggggcgg cgaagcgcag gccgtagacc
     2041 ttcgccagac cgggaaagcc gtcctcccgg ttgaggaact cgtacccgaa cccggccttc
     2101 catcgcgggg cgacgcgcgc cagatccgat atcgcgtgca ggccttcgcg ctgcgcgtcg
     2161 gccccgcgca cggcgacggc gaaggtgttg ttgaagccga acgggggcag ccagacgagc
     2221 ccccagcggc tccgatactc gctcgcggcg gcgcgcgcga tactgtcggc ggacgggccg
     2281 ggggcatgct tcagcacggc cgtgtaggcg gtgcccgtgt attcgacgta gacgtcgatc
     2341 ttccctgcga gaatcgcctc gtgacacacg aacgtgccgc ccagatggag cttgcgctcc
     2401 acggagaggc cggtgcgccg ctcgatctgc tgcgccgcga tttcggccag gaggtcggac
     2461 tcggtgaagt tcttggcgcc caccacaacg cggtcgcggt gggaacagga agcgagggcg
     2521 aacagcgcga gcgcgagcac tgccgatccg tgccctgaag ggccggctcg gcccgaattg
     2581 gcgccctcca gggcgtgccc aaaccaccat ccgcccatcc gccgatccgc ccatccgcct
     2641 accgccacgc aaacctccgc tcgagaagtc ccaataaccc gtccgcggcc agcgccagca
     2701 gcgcggccgg cagggcgccg gccaggatca agcggtcgtc taccaccgcg accccgcgga
     2761 aaatgtagac acccagccct cccgcgccga tcgcggcggc gatcgtcgcg gtgcccacgc
     2821 ccacgacggt cgccacgcga atgccggcga gtatcacgct cgccgccagc ggcagctcca
     2881 ccagccgcag cagctcgccg tcggtcatcc ccatgccgcg ccccgcctcg cgcgccgccg
     2941 gatcgacgcc ggcgattccc gtgtacgtgt tgcgcacgat cgggagcagc gcgtacacca
     3001 ccagcgcgac gatcgcggtc cgcgcgccga ttcccccgag caacggcaac gggatgagga
     3061 acccgaacag cgcgaggctg ggaatcgtct gaaagatgtt ggcgagtccc agcaccggcc
     3121 tccgccaggc gggccgacgc gtgagcagga tgccgagcgg cagcccgatg gccacggcga
     3181 tgaccgtgga tacgatcacg agcaggacgt gctggcccag gagtcccagg acttcccggg
     3241 agttgtccag atagaaccgc agcaggctca tgcggcggcc tcgagaaacg cccgcacctc
     3301 cggatgcgcc gattgccgga actgttccgt gctcccgaga aacaccatcc ggcctccctg
     3361 catcagcccg atgcgcgtgc cgagcagcaa cccttcgcgt acgtcgtgtg tgacgaacac
     3421 catcgccttg ccgaggcgcc cctcgagtcc cttaaactcg tgctgcagct cggcgcgggt
     3481 gatcgggtcg agcgcgccga acggctcgtc gagcagcagc agcgggggat cgacggcgag
     3541 cgcccgggcc acgcccacgc gctgccgctg cccgcccgac agctcgtgcg gccgccggcc
     3601 ggcgaactgc gcgggggcga gccccaccag cgccaggagc tcgcgcaccc gcgcgtcaat
     3661 g
//
LOCUS       DBNGZW010000265         7035 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_775445, whole genome shotgun sequence.
ACCESSION   DBNGZW010000265 DBNGZW010000000
VERSION     DBNGZW010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7035
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_775445"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..696
                     /locus_tag="AC16JC_06365"
     CDS             172..696
                     /locus_tag="AC16JC_06365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164356.1"
                     /translation="MRTLIWILGVVLVPALAAQQDSILARDSAVRDRLQQEIERRFGA
                     VVQQQLDLTPDQADKLRATEERFRPRRRTIQRQQLLLRLGLQGQMRPGIAANPDSVRR
                     LMDGMQANRSELLRLDQEQDREMSGYLTPVQRARYQMLRERLVQRLQELRRERAGQGA
                     GRADLQRPRRRVRP"
     gene            792..1109
                     /locus_tag="AC16JC_06370"
     CDS             792..1109
                     /locus_tag="AC16JC_06370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164357.1"
                     /translation="MFFGIPEWAIGVGFIILAVSVAKWLTGGFTPSGRLRGRRLHPRE
                     IGPALEEMQRRLDAIEEGQQRLGAGEDVQGRLNEVEERLDFVERILAKQRESERVTPP
                     KPS"
     gene            1228..2454
                     /locus_tag="AC16JC_06375"
     CDS             1228..2454
                     /locus_tag="AC16JC_06375"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR5164358.1"
                     /translation="MTVPRRPRASRRRFEGNPRTPREWLLWLTDRWQDFARTPEPLLM
                     DAGAAGEHLVARIRLVVTALVFLIPVVDRFLRPAAREVRVGLIFAGVALLLALLAYRL
                     GRQRLYRPWIGFTTGVLDVTLVSLALVSFLFIGQPFTTLNSRVVYPIYLVAIGASALR
                     FDARICAVTGIVAMLEYLGVVVSTVASQPLLPAAADALAYGRFEWSDQISRLMLLGIA
                     TIMSTSIVLRTRRLQWLSARDRLTDVMHRGVFEDLLASEAARVRRAGGSLALLLVDVD
                     RFGAFNATYGQAAGDRTLRLVAAAIDRASRPRGYVARYGADEFAVLLPESGGDGVADL
                     AEAIRDSLAGAPTHSSRTSRPEPLTVSIGIASLPRDAVDEREVCARAEERLAQAKAAG
                     GDRVVSVTAMQGGGRR"
     gene            2483..2565
                     /locus_tag="AC16JC_06380"
     tRNA            2483..2565
                     /locus_tag="AC16JC_06380"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2516..2518,aa:Leu,seq:caa)
     gene            2763..3518
                     /locus_tag="AC16JC_06385"
     CDS             2763..3518
                     /locus_tag="AC16JC_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164359.1"
                     /translation="MALAAVGARTGEEPLVLVTGACADVFKAQVCTWAKTKGNTLVEA
                     GAVVPLASIENAPAVQPMAWPPVAAAVIDMPVPVRRESGFTQLTIYWEAGGHPPGAFM
                     TPHFDFHFYTVAASERAAMDCKDLSKPVALPVAYGLPDISLPPEMAQMMGVPALIGLC
                     VPQMGMHGLLQSEIERKDAFNGTMVIGYYKGKPIFIEPMLSKAMLMRKQSFDLPIPDV
                     PGLAGAHPTKFHAEYDAAQQAYRFTFSAFTPAT"
     gene            complement(3632..3895)
                     /locus_tag="AC16JC_06390"
     CDS             complement(3632..3895)
                     /locus_tag="AC16JC_06390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164360.1"
                     /translation="MALTARGWEAADAERAAMRIEALYERETEEWKVLLWQIAGGEGT
                     LFLVARRVNGPSVFDSPLEVRARDVASALNEIEADRSEPTGAR"
     gene            complement(4129..4656)
                     /locus_tag="AC16JC_06395"
     CDS             complement(4129..4656)
                     /locus_tag="AC16JC_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164361.1"
                     /translation="MRKLALTLIPLLLLACNQEPAAPALPEPSADLTSATSDWVYWEA
                     GPFSDVDFGPVSCLGDAHMYAFGGVSGRSRWITRPDGRVVEKWWTLTLWDTYHVVVAG
                     ETWWPVDHRARSGVTIYDENGNIVADHGNGAVFILQNEITGALLYYPAHWQFQTDANG
                     ELRVDNFLQGCWVRN"
     gene            5244..>7035
                     /locus_tag="AC16JC_06400"
     CDS             5244..>7035
                     /locus_tag="AC16JC_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VPS10 domain-containing protein"
                     /protein_id="HIR5164362.1"
                     /translation="MRPSLLVAVVLCSSAAAQTPTRLDTALYSGLRWREIGPFRGGRV
                     AAVTGDPVHPLVFYMGATGGGVWKTANAGLSWEPIADSALGSGSIGAIAVAPSDPNVI
                     YVGTGESTVRGNVSPGDGMYRSTDAGKSWSRIGLTDAGQTARIMVDPRDPDRVYVAVF
                     GHAFGPNPTRGVFRSSDGGKTWQKILFKSDSAGAVDVVLDPSNPRVLYAALWQAQRTP
                     WGFTSGGRWSGLFKSTDGGDTWTDLTRNKGLPGGVIGRIGVAVSPLRPERVWALVEAS
                     EGGLYRSDDAGATWTRTSDDHDLRQRAWYFSNVYADPANADAVYVLNTSLLKSVDGGK
                     EFRPIRGSHGDHHALWIDPHDATRMVNGNDGGATVSLDGGASWSTEANQPTAQFYHVI
                     ATTHRPYKVCGAQQDNSPVCIASRTDGGVIGEKDWYDVGGGESGWIAARADDPDVVFA
                     GSYGGYLSRLDVRTGQERNVNAWPDNPMGWAAADLQHRFQWTFPIVLAPTNPNVLYVG
                     SQVVLKSIDEGQHWEAISPDLTRNDKSRQQSSGGPITKDNTSVEYYGTVFSIAPSPKD
                     SALIWVGTDDGLVQLTRDGGKTWQDVTPKEI"
BASE COUNT         1114 a         2335 c         2461 g         1125 t
ORIGIN      
        1 cgaccactac gtgcgcgagg agctgcagga cctctcgacc gatcagctgc gtgaagtgct
       61 gggcagcctg gaccgcatct tgaccgaggc cacggtcgag ccggcggacg aagacctgaa
      121 cgacctgacg acggagcagc tccgggtgct gctgcgctca ttggaggggt aatgcgaacg
      181 ctcatctgga tactcggcgt cgtgctggtg ccggcgctcg cggcgcaaca ggactcgatt
      241 ctcgctcgcg actcggcggt gcgcgatcgg ttgcagcagg agatcgagcg acgcttcggc
      301 gcggtggtgc agcagcagct ggatctcacg ccggatcagg ccgacaagct gcgcgccacc
      361 gaggagcggt tccggccgcg gcgccggacc atccagcggc agcagctgtt gctgcgcctg
      421 gggctgcagg gccagatgcg gcccgggatc gccgccaatc ccgacagcgt gcgccgtctg
      481 atggacggga tgcaggcgaa tcggagcgag ctgctgcggc tcgaccagga gcaggatcgg
      541 gagatgtccg gctatctcac cccggtgcaa cgggcgcggt atcagatgct gcgggagcgg
      601 ctggtgcagc ggctgcagga gctccggcgc gagcgggcgg ggcagggcgc gggccgcgcg
      661 gacctgcagc gtccgcggag gcgggtgcgg ccctagacgg tatgttcctt ccggtgcaac
      721 gcgctcgata cggcggcggt gaaacatcac caccgtcgta ggtatattcc ccggctcgcg
      781 cgggaggagc ggtgttcttc ggcatacccg aatgggccat cggcgtaggg ttcatcatcc
      841 tggccgtctc cgtggcaaag tggctgacgg gcggattcac gccgtccggt cgtttgcgtg
      901 gtcgccgact gcatccgcgg gagatcgggc cggcgttgga ggagatgcag cgtcgtctcg
      961 atgcgatcga ggagggtcag cagcggctcg gcgcggggga ggacgtgcag ggccggctca
     1021 acgaagtcga agagcgcctg gactttgtgg agcgcatcct cgcgaagcag cgcgaatcgg
     1081 agcgggtgac tcccccaaag ccctcgtgaa cggcgggccg cacccggcat tgtgcggcac
     1141 atcacacatc cttgggcagt ctgcagcagc ggcggagacc gtgaaggtcg cctaacggtc
     1201 ccgctccctt ctatacttga tggacctgtg accgtaccac gtcgtccgcg cgcctcgcgg
     1261 cgccggttcg agggcaatcc gcgcacgccg cgggagtggc tgctgtggct cacggatcgc
     1321 tggcaggact tcgcgcgcac ccccgagccg ctgctgatgg atgccggggc ggcgggcgag
     1381 cacctggtgg cgcggatccg gctcgtcgtg accgccctcg tgtttctgat cccggtcgtg
     1441 gaccggttcc tgcggccggc ggcgcgcgag gtgcgggtcg gcctgatctt cgccggggtg
     1501 gccctgctcc tcgcgctcct cgcctatcgg ctgggacgac agcgactcta ccggccatgg
     1561 atcggcttca ccacgggggt gctcgacgtc acgctcgtga gtctcgctct cgtgagtttc
     1621 ctcttcatcg gccagccatt caccacgctc aatagccggg ttgtgtaccc gatctacctc
     1681 gtggccatcg gggcttcggc gctgcggttc gatgcgcgga tctgcgcggt caccggtatc
     1741 gtcgcgatgc tcgagtacct cggcgtcgtc gtgtccacgg tagcgtccca accgctcctg
     1801 ccggcggccg ccgacgcgct cgcctacgga cgattcgaat ggagtgacca gatctcgcgc
     1861 ctcatgctgc tcggcatcgc gaccatcatg agcacgagca tcgtgctgcg cacgcggcgg
     1921 ctgcaatggc tctcggcgcg cgaccggctg accgacgtga tgcatcgcgg cgtcttcgag
     1981 gacctgctcg cttcggaggc cgcgcgggtg cggcgggccg gtggttccct ggcgttgttg
     2041 ctggtggacg tggaccggtt cggcgcgttc aacgcgactt acggacaggc ggcgggggat
     2101 cggaccctgc gcctggtcgc ggccgcgatc gaccgggcga gccggccgcg cggttacgtg
     2161 gcgcgctacg gggccgacga attcgcggtg ctccttcccg agagcggcgg cgacggcgtc
     2221 gcggatctgg cggaggccat tcgagactcc ctggccggag ctcccaccca ttcgtcgcgg
     2281 acgagtcggc cggagccgct gacggtcagc atcgggatcg ccagtctgcc gcgggacgca
     2341 gtggacgagc gggaagtgtg tgcgcgcgcc gaggagcggc tggcccaggc gaaggcggcg
     2401 ggtggcgacc gcgtcgtgag tgtgaccgcg atgcagggcg ggggacgtcg ctagggcggt
     2461 tccgcgtcta tattgcggcg cagcccgggt ggcggaatgg cagacacagg ggactcaaaa
     2521 tcccccgagg gcaacctcat gcgggttcga ctcccgccct gggcatccaa tccacccaaa
     2581 atgtgttccc agcctcggca gccaagcgcg acattgcggt ggccctagcc ccttgtccgc
     2641 agggtgagta cagttcgctt ccgcacttcg tttgctctcg gcccggtccc gggcagtgct
     2701 tccagtcacg ccggagaacc cgcatgtcgc acagtagtgt cccaggcatt tctgccgtcc
     2761 tgttggcgct cgctgccgtg ggcgcccgca cgggcgaaga gcctctcgta ttggtgacgg
     2821 gagcatgcgc cgatgttttc aaggcgcagg tgtgcacgtg ggccaagacc aagggaaaca
     2881 ccttggtcga ggctggtgcg gtagtgccgc tggcaagtat cgagaacgcg ccggccgtcc
     2941 agccgatggc gtggccgccg gtcgctgccg cggtgatcga catgccggta cccgtccgcc
     3001 gggagagcgg cttcacgcaa ctcacgatct actgggaagc gggtggacac ccgccgggcg
     3061 cgttcatgac gccgcacttc gactttcact tctatacggt cgccgcctcc gagcgggcgg
     3121 cgatggactg caaggacctg agcaagcctg ttgcactccc ggtcgcatac ggcctccccg
     3181 acatctcgct gccacctgag atggcccaga tgatgggcgt gcccgcgctg atcggcctgt
     3241 gcgttccgca gatggggatg cacggactgt tgcagtcgga gatcgagcgc aaggacgcgt
     3301 tcaacggcac catggtgatc gggtactaca agggaaagcc gatcttcatc gagccgatgc
     3361 tctccaaggc catgctgatg cggaagcagt cgttcgactt gccgatcccg gacgtcccag
     3421 ggctcgccgg cgcgcatccg accaagttcc acgccgagta cgatgccgca cagcaggcgt
     3481 accggttcac cttctccgcg ttcacccccg ccacctgagc ggtaccgtgc ggcaagcgcc
     3541 gttggacggg taacggctga tcgctccccc acaccctcct ggaagaaccg cctggagggt
     3601 gtaggggacc tgtccggcga cgtcttggcg gtcagcgagc gcccgtcggc tccgaacgat
     3661 cagcttcgat ctcgttcagc gcgctggcga catcgcgggc tcgtacttcc agcggcgaat
     3721 caaagaccga cggtccgttt acgcggcggg cgaccaggaa cagggttccc tcgccgccgg
     3781 cgatctgcca tagcaggacc ttccactcct ccgtctcgcg ctcgtacagc gcttcgatgc
     3841 gcatggcggc ccgctcggcg tccgcggcct cccagccccg tgcggtgagc gccacgatga
     3901 gccggcggcg aagggaattg tccattcgga gcacaagatc cgttcggcgc gtcgtggcgt
     3961 caacggtgga ggatagcggg gagcgccggc atacgctcct ccgtccgtac ccgatatgtc
     4021 gccgggacga ttccagcaaa aacagtgcgc cctcggatcc gatctgcgtc agatccgaag
     4081 acaccaccgc ccggtgcgag accgggcggt gaatacgcgc gcagcagatc agttgcgaac
     4141 ccagcatccc tgcaggaagt tgtccactct cagctcgccg ttcgcgtcgg tctggaattg
     4201 ccaatgggct gggtagtaca gcaacgcgcc ggtgatttcg ttctggagaa tgaagaccgc
     4261 gccgttcccg tggtccgcca cgatgttccc gttctcgtcg tagatcgtga cgccgctgcg
     4321 tgcgcggtga tcgaccggcc accaggtctc gccggccacg acgacatgat acgtgtccca
     4381 cagcgtgagc gtccaccact tctcgacgac tcggccgtca gggcgtgtga tccagcggct
     4441 gcgaccggaa acgcctccga acgcgtacat gtgtgcgtcc cccaaacagg agacggggcc
     4501 gaaatccaca tctgagaacg gtccggcctc ccagtagacc cagtcggagg tcgccgaggt
     4561 cagatcggca gagggctccg gcagcgcggg cgcggccggc tcctggttgc aggcgaggag
     4621 cagcagcggg atgagagtaa gcgcgagctt tcgcatgacg gcctccaacg ctggacagtg
     4681 gaacggcgtt ggcggcgcgg ggtcccgtcg tcgacggacc gtggaggagg gacgcggttc
     4741 tcggcagcgg ggcggcgcgg cgccccgagt atctgctggg tcggcgtctc aggagttcat
     4801 aggtcggttc ctcacgggag aacgcggggt tcagtgtgcc gcgcagcggt caaccgccgg
     4861 tcaatcgcat cggtgggcgc atgagcgcgc cgtgaggttc tgttgaagac gttgcgtcgc
     4921 aggatttgcc ctccgcaaag agcgccatcg agcggcaggt caaggcataa cgacagggcg
     4981 ccccgcagat cttcctccgg ggcgcccagg tgtgaacggg gactgtgtgc ccgggcgcgc
     5041 ggttcgagct tccgtgcctt cgcaatccac cgtgacttcc aacgccaggc ttgcggcgtc
     5101 gtgaagtacg ggggagagga taaccgcccg cgatcaagaa cgcgtcaagt tccggtcacg
     5161 tccgggtccg cctcccgccc cgcgcggtga cctctattgc gtgatatcat tgcgcccgtc
     5221 ttcgaatccc acctggaggc gcaatgaggc cgtcgctgct ggtcgctgtc gtcctgtgtt
     5281 cgtccgccgc cgcccagact cccacccgac tcgacaccgc cctctattca gggctgcgct
     5341 ggcgcgagat cggcccgttc cgcggcggac gggtggccgc cgtgaccggc gacccggtgc
     5401 acccgctggt gttctacatg ggcgccacgg gcggcggcgt ctggaagacg gccaacgcgg
     5461 ggctcagctg ggagccgatc gccgattcgg cgctgggatc gggctcgatc ggcgcgatcg
     5521 ccgtcgcgcc gtccgacccg aacgtcatct acgtcgggac cggcgagtcc acggtgcgcg
     5581 gcaacgtctc tcccggcgac gggatgtacc gctccaccga cgcgggcaag agctggtcgc
     5641 gcatcgggct caccgacgcc ggccagaccg cccgcatcat ggtggacccg cgcgatcccg
     5701 accgtgtgta cgtcgccgtg ttcggccacg cgttcgggcc gaacccgacc cgcggcgtgt
     5761 tccgctcgag cgacggcggc aagacgtggc agaagatcct cttcaagtcg gacagcgcgg
     5821 gcgcggtgga cgtggtgctc gatccgtcca acccgcgcgt gctgtatgcc gcgctgtggc
     5881 aggcgcagcg caccccctgg ggcttcacca gcggcggccg ctggagcggt ctcttcaagt
     5941 ccaccgatgg cggcgacacc tggacggacc tcacccgcaa caagggactc cccggcggcg
     6001 tgatcggccg catcggcgtc gcggtttcac cgctgcggcc ggagcgggtc tgggcgctcg
     6061 tcgaggcgag cgagggaggg ctctatcgct ccgacgacgc gggtgcgacc tggacgcgga
     6121 ccagcgacga ccacgatctg cgacagcgcg cctggtattt cagcaacgtg tacgccgatc
     6181 cggcgaacgc tgatgcggtg tacgtgctca acacctccct gctcaagtcc gtggacggcg
     6241 gcaaggagtt ccggccgatc cgcggctcac acggcgacca tcacgccctg tggatcgatc
     6301 cgcacgacgc cacccggatg gtgaacggca acgacggcgg ggccacggtg agcctggacg
     6361 gcggcgccag ctggagcacc gaagccaacc agcccacggc gcagttctac cacgtgatcg
     6421 ccacgacgca ccgtccgtac aaggtgtgcg gcgcgcagca ggacaacagc ccggtgtgca
     6481 tcgccagccg gaccgacggc ggagtgatcg gcgagaagga ctggtacgac gtgggcgggg
     6541 gggagagcgg ctggatcgcc gcccgcgccg acgacccgga cgtcgtgttc gcgggcagct
     6601 acggcggcta cctcagccgc ctcgacgtgc gcaccggcca ggagcgcaac gtcaacgcgt
     6661 ggcccgacaa cccgatggga tgggcggcgg ccgacctgca gcaccgattc cagtggacct
     6721 tccccatcgt gctcgcgccc accaacccga acgtgctcta cgtcggctcc caggtcgtgc
     6781 tcaaatccat cgacgagggg cagcactggg aggcgatcag ccccgacctc acgcgcaacg
     6841 acaagtcgcg ccagcagtcc tcgggcggtc ccatcaccaa ggacaacacg agcgtcgagt
     6901 actacgggac ggtgttcagc atcgcgccgt cgcccaaaga ctcggcgctc atctgggtag
     6961 gcacggacga cgggctggtg cagctcaccc gcgacggcgg caagacgtgg caggacgtga
     7021 cgccgaagga gatca
//
LOCUS       DBNGZW010000266         5308 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1658306, whole genome shotgun sequence.
ACCESSION   DBNGZW010000266 DBNGZW010000000
VERSION     DBNGZW010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5308)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5308)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5308
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1658306"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="AC16JC_06405"
     CDS             complement(<1..688)
                     /locus_tag="AC16JC_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR5164363.1"
                     /translation="MTARDWAFAGALGVGAGSGLLYLARNRLRRTAQRAVLRFRTRLA
                     RYHLQQRRVARAALLDDPVVGVAVAEHAREQGVPQPRVWRLVASYVDEIVPYLNVLSY
                     YKLGYNAAKLVVNLLYKVSVDYQDEAALARIPKRDVVVYLMNHRSNADYVVVAYVLAR
                     GTHVSYAVGECARAWPLEYVFKSFGAYFIRRRFRQPLYHAVLERYVQLITTNGVTQGI
                     FPEGGLTRDGR"
     gene            complement(685..1734)
                     /locus_tag="AC16JC_06410"
     CDS             complement(685..1734)
                     /locus_tag="AC16JC_06410"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HIR5164364.1"
                     /translation="MTDDASLNLFQQQEANRRRTVALVIGFVLFFAWLGFGGDLILHL
                     ATTDAAPDAYHHSFPWFGLVLTAIGAGIARYGFATGPNKVLWSTGAREVTDPQTDKER
                     QLVNVVDEMAIAAGVPRPRIWLVPDADPNAFATGLDQLHSHMAVTQGLLDLCSRDELQ
                     AVVGHELGHVKNLDVRLMTTLAALVGAVALMHDGMGRLMRGGFRVGGGGGSRGGGGGG
                     KKGGLGQLAIVLLVLWVITWILAPIISQLLAMAVSRKREYLADAMGAQFTRNPMALAT
                     ALQKIENADAPTSSIKRGAAHLCIADPLGRRMNMREGGVANLLATHPPMAVRIARLKA
                     MAYQQLKAEGQFPAA"
     gene            complement(1731..2315)
                     /locus_tag="AC16JC_06415"
     CDS             complement(1731..2315)
                     /locus_tag="AC16JC_06415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR5164365.1"
                     /translation="MGLILLIVLAAVVLYGIAAYNGLINLKNQTLNAFKQIDVQLKRR
                     HDLIPNLVNAVKGAMDFERQTLEAVIQARNQAITAGANAGPGAMQQVAAAENQLTAAL
                     SRFNVVVEQYPQLKATGNVAQLQEELTSTENKISFARQMYNDTATQYNTKQQQFPTNL
                     FAGIAKASPAELWEITDEGERAVPQVDLSMKPKA"
     gene            complement(2350..2988)
                     /locus_tag="AC16JC_06420"
     CDS             complement(2350..2988)
                     /locus_tag="AC16JC_06420"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR5164366.1"
                     /translation="MTAATQRDPIHILREYRNLAPWNLHDLSMVVGAVLQASAVTPIN
                     AAARATPSQRTIRFYVAKGLVSPPEGRGTAATYTYRHFLQLLCIKLRQMEGATLAAII
                     REMQDQTGDVLERRAAQTLGAALPPPDRLSLRGGEGLPRGRSGRAINAWMARDVEREP
                     GGPSSWRRIPVARGVELHLDADHPLARLGPDEAGVAEMVRQALAKLLPQWPA"
     gene            3145..3570
                     /locus_tag="AC16JC_06425"
     CDS             3145..3570
                     /locus_tag="AC16JC_06425"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HIR5164367.1"
                     /translation="MRGFTLIELLVVLMILAVAASVAVPRLGSALDRIATDAAARDVT
                     TALATARAAAVQQGTRARLRIAADSLRIDREGAQGWEPYARWPGPSASGVSLQVSNPE
                     IVFGPLGLGWGLANTRVELRRGSQIETVTTSRLGRVKRG"
     gene            complement(3779..4831)
                     /locus_tag="AC16JC_06430"
     CDS             complement(3779..4831)
                     /locus_tag="AC16JC_06430"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR5164368.1"
                     /translation="MPNRPPPPSVALLIVDDEEPIRNALKRFLEGERFAVHTAASGQE
                     ALTALQQHDIALMLADIRMPGMSGIDLVPQALEVAPDLAVVMLSAVNDATTAALCMQR
                     GAMDYLTKPIELDDLARAVQRALRRRAALVESRHLEVQLKEEWARERAEAHRVTIATL
                     EALVNAMEARDPYLRGHSARVADLAATIAAHLRLSDDEVEQIRLAGRLHDIGKIGTRE
                     SVMNKQGPLTPDEYEHVKQHVIIGSQILSPLVHLGPVLGSVRSHHERWDGTGYPDGLR
                     GEEIPIGARIIGAAEIYDALCTSRPYQEKLPSPQAVRRMGDLAGTVLDPKVFAALAEV
                     VTRRRSLVFLEGAEGT"
     gene            complement(4863..>5308)
                     /locus_tag="AC16JC_06435"
     CDS             complement(4863..>5308)
                     /locus_tag="AC16JC_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HIR5164369.1"
                     /translation="QIEDYVAAGRADAGLLIHEGQLTFAGRGLHLWVDLGEWWHAETG
                     LPLPLGGNVARRDLGEPLLAAIARDLKASIEYGLAHRAAALAHAQSYSRGLDPAQTDR
                     FVGMYVNDFTVDYGERGRRAVEELLARGHRAGLLPAPVALQFIGA"
BASE COUNT          769 a         1767 c         1910 g          862 t
ORIGIN      
        1 ggcggccgtc gcgcgtgagt cccccttcgg ggaagatgcc ctgcgtgacg ccgttggtcg
       61 tgatgagctg gacgtagcgc tcgagcaccg cgtggtagag cggctggcgg aagcggcgcc
      121 ggatgaagta cgcgccgaac gacttgaaga cgtactcgag cggccacgcg cgcgcacatt
      181 ctcccacggc gtagctcacg tgggtaccgc gcgccagtac gtaggcgacc accacgtagt
      241 ccgcgttgga gcgatggttc atcaggtaca cgaccacgtc ccgcttgggg atgcgagcga
      301 gtgccgcctc gtcctggtag tccacgctca ccttgtagag caggttgacc accagcttcg
      361 ccgcgttgta cccgagcttg tagtaggaga gcacgttgag ataggggacg atctcgtcga
      421 cgtagctcgc gacgagccgc cacacccggg gctggggaac tccctgctcg cgcgcgtgct
      481 cggccacggc gacgcccacg acggggtcgt ccagcagggc ggcccgggcg acgcggcgct
      541 gctgcaggtg atagcgggcc aggcgcgtgc ggaaccgcag cacggcgcgc tgcgccgtac
      601 ggcggagtcg attgcgggcg agatagagga ggccgctgcc ggcgccgact cccaacgcgc
      661 ccgcgaaggc ccagtcccga gcggtcacgc ggcggggaat tgtccttcgg ctttgagttg
      721 ctggtaggcc atggccttga gtcgggcgat gcgcacggcc atcggcgggt gggtcgccag
      781 caggttggcc acgccgccct cgcgcatgtt catgcgccgg ccgagcggat cggcgatgca
      841 gaggtgcgcg gctccccgct tgatcgaact ggtgggggcg tccgcgtttt cgatcttctg
      901 gagcgccgta gcgagcgcca tcgggttgcg ggtgaactgc gcgcccatcg cgtcggcgag
      961 gtattcccgt ttccggctca cggccatcgc gaggagctgc gagatgatcg gcgccaggat
     1021 ccaggtgatg acccacagca cgagcagcac gatggccagc tggccgaggc cgcccttctt
     1081 gccgcccccc ccgccgccgc gagagccccc cccgccaccc acccggaagc cgccgcgcat
     1141 caggcgtccc atgccgtcgt gcatgagggc gaccgcgccc accagggcgg ccagggtggt
     1201 catgagtcgc acgtcgaggt tcttcacgtg tcccagctcg tggcccacca cggcctggag
     1261 ctcgtcccgc gagcacaggt cgagcaggcc ctgcgtgacg gccatatggg aatgcaactg
     1321 gtcgagcccg gtggcgaacg cgttcggatc ggcgtcgggg acgagccaga tgcggggacg
     1381 ggggacgccg gccgcgatcg ccatctcgtc cacgacgttg accagctggc gctccttgtc
     1441 ggtctgcggg tcggtcacct cccgcgcgcc ggtggaccag agcaccttgt tcggaccggt
     1501 cgcgaagccg tagcgggcga taccggcgcc gatggcggtg aggacgaggc cgaaccaggg
     1561 gaacgagtgg tgataagcat ccggggcggc atcggtggtg gccaggtgga ggatgagatc
     1621 accgccgaac ccgagccaag cgaagaacag cacgaagccg atcaccagcg cgacggtgcg
     1681 ccggcgattg gcctcctgct gctgaaacag gttgagcgaa gcgtcgtccg tcacgccttg
     1741 ggtttcatcg agagatcgac ttgcgggacg gcgcgctcgc cctcgtcggt gatctcccac
     1801 agctcggccg gcgacgcctt ggcgatgccg gcgaacaggt tggtggggaa ctgctgctgc
     1861 ttggtgttgt actgggtcgc cgtgtcgttg tacatctgcc gggcgaagct gatcttgttc
     1921 tcggtggacg tgagctcttc ctgcagctgc gccacgttac cggtcgcctt gagctgcgga
     1981 tactgctcca ccaccacgtt gaagcgcgac agcgcggcgg tcagctggtt ctcggccgcg
     2041 gcgacctgct gcatcgcccc gggtccggcg ttcgccccgg cggtaatcgc ctgattccgg
     2101 gcctggatca cggcctcgag ggtctggcgc tcgaagtcca tggcgccctt caccgcgttc
     2161 acgagattgg ggatcagatc gtgccggcgt ttcagctgca cgtcgatctg cttgaacgcg
     2221 ttgagcgttt ggttcttgag attgatcagt ccattgtagg cggcgatccc gtacagcacg
     2281 acggccgcca ggacgatcag gaggatcagc cccatggttg ctcctcgagg aaggtggggt
     2341 gagcgatgct tacgcgggcc actgcggcaa tagtttcgcc agcgcctgcc gcaccatttc
     2401 cgccacgccg gcttcgtccg ggccgaggcg ggcgagcggg tggtccgcgt cgagatgcag
     2461 ctccacgccc cgggctacgg ggatgcgccg ccagctgctg gggccgccgg gctcgcgctc
     2521 cacgtcccgc gccatccacg cattgatggc gcgcccggac cggccacgcg gcagtccctc
     2581 gccgccgcgc agcgacaggc gatcgggcgg cggcagcgcg gccccgagcg tctgggcggc
     2641 gcgtcgctcc agcacgtctc ccgtctgatc ctgcatctcg cgaatgatgg ctgcgagggt
     2701 cgctccctcc atctggcgca gcttgatgca cagcagctgg aggaagtgcc ggtaggtata
     2761 ggtcgcggcc gtgccgcggc cttcgggagg cgacacgagc cccttcgcca cgtagaaccg
     2821 gatggtccgc tggctcgggg tggcccgggc ggcggcgttg atcggggtca ccgcggacgc
     2881 ctgcagcacc gcccccacga ccatcgacag gtcgtggagg ttccaggggg cgagattgcg
     2941 atactccctc aggatgtgga tgggatcgcg ctgcgtcgcg gcggtcatcg gctcccagcc
     3001 cggaccttgc ttggacagcc aaacaatagc ggttcccggc tcggggggtt agcccgcgcg
     3061 cagcgccgcc acaccgggct ttttcgatcg tggcggcgtg aaacggatga cagtcggcgc
     3121 gccggagcgc tgtaccatcg ggccgtgcgc ggattcaccc tcatcgagct gctcgtggtc
     3181 ctcatgatcc tggcggtggc ggcctcggtg gccgtgccgc gcttgggatc ggccctggac
     3241 cgcatcgcca cggatgcggc agcgcgcgac gtcacgaccg ccctggccac ggcgcgagcg
     3301 gcagcggtgc agcagggaac gcgcgcccgc ctccggatcg ccgccgactc gctgcgaatc
     3361 gaccgggagg gcgcgcaggg ttgggagccc tacgctcgct ggccgggccc gtctgcttcg
     3421 ggcgtaagcc tccaggtgtc gaacccggag atcgtctttg ggccgctggg gttgggctgg
     3481 ggcctggcca atacccgggt cgagctgcgt cgcgggtccc agattgaaac ggtcacgacc
     3541 tcacgtctgg gacgtgtcaa gcggggctag agggggaacc gcgaatcggc tgcaacgttt
     3601 tcgagggagc gcgcatcaaa gggagtgacg cggccgccga agggtgcaac aaatcccgcg
     3661 gtggccgcgt atgatgggcg cctgacattc atagatccaa gtggtcgcgg ccgaggtgtg
     3721 acggccactt cgcccgaccc gccagcccgg ggtgcgcaag cccccgggtt tggtcttttc
     3781 aggtcccctc tgctccttcg agaaacacca gcgagcggcg ccgggtgacg acctcggcga
     3841 gggcggcgaa gaccttagga tcgagcacgg tccccgccag gtcccccatg cgccggaccg
     3901 cctgcgggga agggagcttc tcctggtagg gccggctggt gcacagggca tcgtagatct
     3961 ccgctgcgcc gatgatgcgg gccccgatcg ggatctcctc gccgcgcagg ccgtcggggt
     4021 atccggttcc gtcccagcgc tcgtgatggc tgcggacgct ccccaggacc ggtcccaggt
     4081 gcaccagcgg ggacaggatc tgcgagccga tgatcacgtg ctgtttgacg tgctcgtact
     4141 cgtccggcgt gagcggcccc tgcttgttca tcacactttc gcgcgtcccg atcttcccga
     4201 tgtcgtgcag tcgtccggcg agtcggatct gctcgacctc gtcgtcggac aggcgcaggt
     4261 gggcggcgat ggtggcggcg aggtcggcca cgcgggcgga atggccgcgg aggtaggggt
     4321 cccgcgcttc catcgcgttc accaacgcct cgagggtcgc aatcgtgaca cggtgcgcct
     4381 cggcgcgctc gcgcgcccac tcctccttca gctgcacttc gaggtgccgg ctctcgacca
     4441 gggccgcgcg ccggcgcagg gcgcgctgca cggcccgggc gagatcgtcg agctcgatcg
     4501 gcttggtgag gtagtccatc gcgccccgct gcatgcacag cgcggcggtg gtcgcgtcgt
     4561 tgacggccga gagcatgacg accgccagat cgggagcgac ctcgagcgcc tgcggcacga
     4621 ggtcgatacc gctcatgccg ggcatccgga tgtcggccag catgagggcg atgtcgtgtt
     4681 gttggagcgc ggtgagcgcc tcctggccgg aggccgccgt gtgcaccgcg aaccgctccc
     4741 cctcgaggaa ccgtttgagg gcgttccgaa tcggctcttc gtcgtccacg atcaacaggg
     4801 cgacggaggg gggaggcggg cgattcggca cgtgattcga tccctctggt gaggggtcga
     4861 agttacgcgc cgatgaactg taacgccacg ggagccggca acagtcccgc ccggtggccg
     4921 cgcgccagca actcctccac ggcccggcgt ccccgctcgc cgtaatccac ggtgaagtcg
     4981 ttcacgtaca tgcccacgaa ccggtcggtc tgggcgggat cgagcccccg gctgtagctc
     5041 tgcgcgtgcg ccagcgccgc ggcacggtgg gcgagcccgt attcgatgct ggctttcagg
     5101 tcgcgggcga tcgccgcgag cagcggctcg cccaggtcgc gtcgcgccac gttgcctccc
     5161 agcggcaggg gcaggccggt ctccgcatgc caccattctc ccaggtccac ccagaggtgc
     5221 agtccgcggc cggcgaaggt gagctggcct tcgtggatga gcaggccggc gtcggcccgg
     5281 cccgcggcga catagtcctc gatctggt
//
LOCUS       DBNGZW010000267         3378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_635475, whole genome shotgun sequence.
ACCESSION   DBNGZW010000267 DBNGZW010000000
VERSION     DBNGZW010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3378
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_635475"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2088
                     /locus_tag="AC16JC_06440"
     CDS             <1..2088
                     /locus_tag="AC16JC_06440"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5164370.1"
                     /translation="GQAVLGLAVLLSAYPAIRLAAQDSSPPQPPAPTPLAPVRFPPFR
                     ESLLPNAMTLLVVENHEQPVISVNLSFRAGELYDPAGKEGTAELVAQVLTKGTPTRTA
                     DQIAAQIEGAGGSLSASSDDDFLTVSVNVLSDRADLAFDLLGDVVRRATYPADELELA
                     RTQALSALALSLSQPANVADRFFRADIYGRHPYGRSPAAESYKAISRDDVKRFAANRI
                     RPGGALLVIAGDVTALRARALALKAFGGWTGAPAVVAPPPAPPVHSATAILLVHRPGS
                     VQGNIEIGNTTFGPTDPGYYAARVATQVLGGGADARLFLILREQKSWTYGAYAGLVRH
                     RGLGAWQATFEGRTDVVDSALVEMLHQIDRMRTETVPDSELANTKGFLIGSFPLTIET
                     PEQIAGVVANASLLGLGADYVRLYRERLAAVTPAQARAAAARTYHRAALSIIVVGDGE
                     KLYDRLKAVAPVHIVDVDGKPLKPEDLRAPTGAPTLDHAQIVTRTDSFQIVIQGTPNG
                     ALTSRTLVSADSIVYQENTVIAAGMVQQGTTVRFNPADLAVTRVDQTGRVQGQPAEIH
                     LTYGAGRVKGTSTTPQPGGSSATLQIDTTVAPGTYDDNALSFVLPALPLADGRSFARN
                     VFESGKGVTQVMQIKVSDGGSMTVPAGTFPVFRLDITGGQVPTAFYVTRDAPRRIVKI
                     ELVGAPFVFELVK"
     gene            2112..>3378
                     /locus_tag="AC16JC_06445"
     CDS             2112..>3378
                     /locus_tag="AC16JC_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR5164371.1"
                     /translation="MRSSWILLLPLLASCASASARPATQAPAPAPTPAAAAETTSAQR
                     PQYPSTYLRHPNPPVLIRNATIMTATGQEIAGGSILFRDGRIVSVGTTVEAPADAVVV
                     DGSGKYVTPGIIDDHSHLGVYAAPGTDAESDGNEATNPVTAEVWAEHSFWPQDPQIPL
                     AIAGGITTIQALPGSANLIGGRSAVLKLIPARTVQEMKFPGAPYGLKMACGENPKRVY
                     RTRGPSTRMGNMAGYRAAFIQAEGYRQRWDKWNRTHQGDPPQRDLKLESLAEVLRGNM
                     LVQNHCYRADEMAQMLDLAHEFGFTIRSFHHAVEAYKIADLLAREGTASSVWADWWGF
                     KEEAMDGIHENAALLQAAGARVVIHSDDPGGIQRLNQEAGKAMYAGNRAGVPVTRDQA
                     LRWITANPAWVLGIDSIVGTLEPGKMADVI"
BASE COUNT          547 a         1213 c         1135 g          483 t
ORIGIN      
        1 ggtcaggcgg tcctcggatt ggcggtgctg ctatccgcct atccggcaat ccgcctagcc
       61 gcccaggatt cttccccccc gcagccgccg gcgccgacgc cgctggcgcc ggtgcgattc
      121 ccgccgttcc gggagtcgct gctgcccaac gccatgaccc tgctcgtggt ggagaaccac
      181 gagcagccgg tcatctccgt caatctgagc ttccgcgccg gcgagctgta cgatcccgcc
      241 ggcaaggagg gcactgccga gctcgtcgcc caggtgctca ccaagggaac ccccacccgc
      301 accgccgacc agatcgcggc gcagatcgag ggtgccggtg gaagcctgag cgcatcctcg
      361 gacgacgact ttctcacggt gtcggtcaac gtgttgagcg accgtgccga cctggcgttc
      421 gacctgctgg gcgacgtagt acggcgcgcg acctaccccg ccgacgagct ggagctggcg
      481 cgcacgcagg ccctctcggc cctggcgctc tcgctgtcgc agcccgcgaa cgtggccgac
      541 cgtttcttcc gcgcggacat ctacgggcgt cacccctatg gccgcagccc ggcggcagaa
      601 tcctacaagg cgatctcccg ggacgacgtg aagcggttcg cggcgaaccg catccggccg
      661 ggcggcgccc tcctggtcat cgccggcgac gtcaccgcgc tccgcgcccg tgcgctcgcc
      721 ctcaaggcat tcggcggatg gaccggggcg ccggcggtcg tggcgccgcc gcccgctccc
      781 ccggtgcatt cggccaccgc catcctgctc gtgcaccggc cgggatccgt gcaaggcaac
      841 atcgagatcg gcaacaccac cttcgggccg accgaccccg ggtactatgc ggcgcgcgtc
      901 gccacccagg tgctcggcgg cggtgcggac gcccggctgt tcctcatcct gcgcgagcag
      961 aagagctgga cgtacggcgc gtacgccggc ctggtgcgcc accgcgggct cggcgcctgg
     1021 caggcgacgt tcgaaggccg taccgacgtc gtggactcgg cgctcgtgga aatgctgcac
     1081 cagatcgacc gcatgcgcac cgagacggtg cccgacagcg agctcgcgaa caccaagggg
     1141 ttcctcatcg gctcgtttcc gctcaccatc gagacgccgg aacagatcgc cggcgtggtg
     1201 gccaacgcca gcctcctggg gctgggtgcg gactacgtgc ggctgtaccg cgagcggctg
     1261 gccgcggtga cgcccgcgca ggcgcgcgcc gccgccgcac ggacctatca ccgcgcggca
     1321 ctcagcatca tcgtggtggg agacggtgag aaactctacg atcgcctcaa ggccgtcgcc
     1381 ccggtccaca tcgtggacgt ggacggaaag ccgctcaagc cggaggatct cagagcaccg
     1441 acgggagctc ccacgctcga ccacgcccag atcgtgacgc ggaccgacag cttccagatc
     1501 gtcatccagg gcacccccaa cggcgccctg acgtcccgga cgctcgtgtc cgccgactcg
     1561 atcgtttacc aggagaatac ggtcatcgcc gccgggatgg tgcaacaggg cacgacggtg
     1621 cgcttcaacc ccgcggacct ggcggtgacg cgggtcgacc agacggggcg cgtacaggga
     1681 cagcccgccg agattcacct cacctacggc gccggccggg tgaagggaac gtccacgacg
     1741 cctcaaccgg gcggcagctc cgccaccctg cagatcgata cgacggtagc gcccggcacc
     1801 tacgacgaca acgcgctcag cttcgtgctg ccggcgctgc cgctggcgga cggccggagc
     1861 ttcgcccgga acgtgttcga gtcgggcaaa ggcgtcacgc aggtcatgca gatcaaggtc
     1921 tccgacggcg gcagcatgac cgtgcccgcg ggcacgttcc cggtcttccg gctcgacatc
     1981 acgggtggac aggtgccgac ggccttctac gtgacgcggg acgcgccccg tcgcatcgtg
     2041 aagatcgagc tggtcggggc tccgttcgtg ttcgaactgg tgaaatagtc ctcgcgcggg
     2101 agattccttt catgcgctcc tcctggatcc tgttgctgcc gctcctggca tcgtgcgcca
     2161 gtgcgagcgc ccggcccgcc acccaggccc ctgccccggc cccgaccccg gcggccgcgg
     2221 cggagacgac cagcgcacaa cgtccccagt atccctcgac ctacctgcgc catcccaacc
     2281 cgcccgtcct gatccgcaac gcgacgatca tgaccgccac gggacaggaa atcgccggcg
     2341 gatcgatcct gttccgcgac gggcgcatcg tgagcgtcgg cacgacggtc gaagcgcccg
     2401 ccgatgcggt ggtggtggac ggcagcggca agtacgtgac gccgggcatc atcgacgacc
     2461 actcgcacct gggggtgtac gcggcgccgg ggaccgacgc cgagtccgac ggcaacgagg
     2521 cgaccaatcc ggtcaccgcc gaggtttggg ccgagcattc gttctggccg caggatccgc
     2581 agattccgct cgccatcgcg ggcggcatca ccacgatcca ggcgttaccc ggttcggcga
     2641 acctcatagg cgggcgcagc gccgtgctca agctgatccc cgcgcggacc gtgcaggaga
     2701 tgaagttccc cggcgcgccc tatggcctga agatggcgtg cggtgagaac cccaagcggg
     2761 tgtaccgcac gcgcgggccc tccacgcgga tgggtaacat ggcggggtac cgtgccgcat
     2821 tcatccaggc cgagggatac cgccagcgct gggacaagtg gaaccgcacc catcagggag
     2881 atccgccgca gcgcgacctg aagctcgagt cgctggccga ggtactgcgc ggcaacatgc
     2941 tggtgcaaaa ccactgctat cgggcagacg agatggcgca gatgctggat cttgcgcacg
     3001 aattcggctt cacgatccgc tcgttccacc atgcggtcga ggcgtacaag atcgccgatc
     3061 tcctggcccg cgaaggcacc gcctcctcgg tgtgggccga ctggtggggg ttcaaggaag
     3121 aggcgatgga cggcattcac gagaacgcgg cgctgctcca ggccgccggg gcccgggtgg
     3181 tgattcattc cgatgacccg ggcggcatcc agcggctcaa ccaggaggcg ggcaaggcga
     3241 tgtacgcggg caaccgcgcc ggcgtccccg tcacgcgcga ccaggcgttg cgctggatca
     3301 ccgcgaaccc cgcctgggtg ctggggatcg actcgatcgt cggcacgctc gagccgggga
     3361 agatggccga cgtcatcg
//
LOCUS       DBNGZW010000268         2548 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_976998, whole genome shotgun sequence.
ACCESSION   DBNGZW010000268 DBNGZW010000000
VERSION     DBNGZW010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2548)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_976998"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..464
                     /locus_tag="AC16JC_06450"
     CDS             <1..464
                     /locus_tag="AC16JC_06450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164372.1"
                     /translation="IACGPKSPCGSGTVRRDLVRFSQPYAEHWIVAHGDSLTLPDSTL
                     GDRFKLTDIILGTDTVSVARECLYRGTLVFAVPRAETLAVTWFGVPEHVTVFGWPADL
                     GPFAGLNASWWGRDSLRGAILFDERFVTQVRPGATAQFTAGRSRPTVPAAR"
     gene            493..894
                     /locus_tag="AC16JC_06455"
     CDS             493..894
                     /locus_tag="AC16JC_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164373.1"
                     /translation="MAAGTGSRVGVLGGLAGVVIGGFTWVVVYGLHLHAPLVALSGAA
                     AAAALWLVAATTYDRFSERRLALLGGLFIAVAAVDWLYIGLLMPRLPDLPANPAIGIS
                     RASLRIVQPILIAASVAGTALVLWDLFRRKP"
     gene            896..1381
                     /locus_tag="AC16JC_06460"
     CDS             896..1381
                     /locus_tag="AC16JC_06460"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5164374.1"
                     /translation="MALSSAERTAMIDRYERGPALLTATLAKIPKDALQWRPAPGRWS
                     AHEIIVHCADSETNGASRIRYLFAEAAPMIAGYDQDRWATALDYHQLPLEPALATVVA
                     VRAHTVAVLRRATEADWARTGTHTEVGRYGAESWLKVYAEHLEKHSRQLEGNLAAWSK
                     R"
     gene            1378..1779
                     /locus_tag="AC16JC_06465"
     CDS             1378..1779
                     /locus_tag="AC16JC_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002518567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HIR5164375.1"
                     /translation="MRGFLTRLVITAAGLWLAQAIIPGVRISGVGTLALAALLLGVVN
                     ALVRPVLFILTLPLTVVTLGLFLLVLNGISLALVAWLLPHFAVSSLWAATLGALVVGI
                     TSWFANGFVGSSGKLERYHHSGIEVPGRRVD"
     gene            1779..2147
                     /locus_tag="AC16JC_06470"
     CDS             1779..2147
                     /locus_tag="AC16JC_06470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011984723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HIR5164376.1"
                     /translation="MDRGFAVAGSLSALVAVAAGAFGAHGLRQRLPGDLLAVFETGAR
                     YQMYHALALLFVAWAVTHWPAAPVRAAGWLFIGGTAVFSGSLYLLALTGARWWGAVTP
                     LGGVLFLAGWLSLAIGIWRR"
     gene            2135..>2548
                     /locus_tag="AC16JC_06475"
     CDS             2135..>2548
                     /locus_tag="AC16JC_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164377.1"
                     /translation="MAALSRRAQLAFAAGFVLYACIVIPLGVRKGDDVQTEITQAHRI
                     LDGLPLYNTPQTQGVWWTPFALLVVLPAALLADWSLALAKACWGALGMVALAWSVYET
                     GRRWGWRPAIGALGVVLFPVHNNFHHLNIETLLLAL"
BASE COUNT          293 a          961 c          867 g          427 t
ORIGIN      
        1 tcatcgcctg cggtcccaag agtccgtgcg gctccgggac cgtccgccgc gacctggtgc
       61 gcttctccca gccctacgcc gagcactgga tcgtggcgca cggcgattcc ctcacccttc
      121 ccgattccac cctcggcgac cgcttcaagc tcaccgacat catcctcgga acggacacgg
      181 tgtcggtggc gcgggagtgc ctgtaccgcg gcacgctggt gttcgcggtg ccgcgcgccg
      241 agacgctcgc ggtgacctgg ttcggcgtcc ccgagcacgt gacggtgttc gggtggcccg
      301 ccgacctcgg tccgttcgcc ggactcaacg cctcgtggtg gggacgcgac agccttcgcg
      361 gggcgatcct gttcgacgag cggttcgtca cgcaggtccg tcccggcgcg acggcgcagt
      421 tcaccgccgg ccgcagccgc cccacggtcc cggccgcgcg ctagcccggc gccgcgatta
      481 tcttctcccg ccatggcggc gggaaccggt tcgcgcgtcg gagtcctggg cggcctcgcc
      541 ggagtcgtca tcggcggctt cacctgggtg gtggtgtacg ggctccacct ccacgccccg
      601 ctggtcgccc tgtcgggagc cgcggcggcc gccgccctgt ggctcgtcgc cgccacgacg
      661 tacgaccgtt tttccgagcg ccggctcgcg ctgctgggcg gcctgttcat cgcggtcgcc
      721 gccgtcgact ggctctacat tggcctgctg atgccgcggc tccccgatct gcccgccaat
      781 cccgccatcg gcatctcccg cgcctcgctc cgtatcgtcc agccgatcct gatcgccgcg
      841 tcggtggcgg gcaccgcgct ggtcctgtgg gatctcttcc gccggaagcc ctgacatggc
      901 actcagctca gccgaacgca ccgcgatgat cgaccgctac gagcggggcc ccgccctgct
      961 cacggccacc ctcgccaaga tccccaagga cgcgctgcag tggcgccccg cgcccggccg
     1021 atggtcggcg cacgagatca tcgtgcactg cgccgactcc gagacgaacg gggccagccg
     1081 catccgttac ctcttcgccg aagccgcacc gatgatcgcc ggctacgacc aggaccgctg
     1141 ggccacggcg ctggactacc accagctgcc gctcgagccg gcgctcgcca cggtcgtggc
     1201 ggttcgcgcg catacggtgg cggtgctgcg ccgcgccacc gaggccgact gggcgcggac
     1261 gggcacgcac accgaagtcg gccgctacgg cgccgagtcc tggctcaagg tctacgccga
     1321 gcatctcgaa aagcactcgc gccagctcga gggcaacctc gccgcctgga gcaagcggtg
     1381 agaggcttcc tgacgcgcct cgtgatcacg gcggccggcc tgtggctggc ccaggcgatc
     1441 atccccggcg tacggatctc cggggtcggc acgctggcgc tcgcggcgct gctgctcggc
     1501 gtggtgaacg ccctggtgcg gccggtgctg ttcatcctca ccctgccgct cacggtggtc
     1561 acgctcggcc tcttcctgct cgtcctgaac gggatctcgc tggccctcgt ggcctggctg
     1621 ctgccgcact tcgcggtgag cagcctgtgg gcggcgacgc tcggcgcgct cgtcgtcggg
     1681 atcacgagct ggttcgccaa cgggttcgtg ggcagctctg gcaagctcga gcgctatcac
     1741 catagcggta tcgaagtccc gggacgccgc gtcgattaat ggaccgcggg ttcgcggtag
     1801 ccggcagtct ctccgccctg gtcgcggtgg cggccggcgc gttcggcgca cacgggttgc
     1861 gccagcgcct cccgggcgac ctcctcgccg tgttcgagac cggggcgcgc taccagatgt
     1921 accacgcgct cgccctgctg ttcgtcgcct gggccgtgac gcattggccc gctgcaccgg
     1981 tgcgcgcggc cggctggctg ttcatcggcg gcaccgcggt gttctcgggc agcctctatc
     2041 tcctcgccct cacgggcgcg cgctggtggg gcgccgtcac gcccctcggc ggggtgttgt
     2101 tcctcgccgg ctggctgtcg ctggcgatcg ggatatggcg gcgctgagcc gccgggcgca
     2161 gctggcgttc gccgccggct tcgtcctcta cgcctgcatc gtgattccgc tcggcgtccg
     2221 caagggcgac gacgtccaga ccgagatcac ccaggcccac cgcatcctcg acggcctgcc
     2281 gctctacaac acgccgcaga cgcagggcgt gtggtggacg ccgttcgcgc tgctggtggt
     2341 gctgccggcg gcgctgctcg ccgactggag cctcgccctc gccaaagcgt gctggggcgc
     2401 gctcggcatg gtggcgctcg cctggagcgt gtacgaaacc gggcggcgct ggggctggcg
     2461 ccccgcgatc ggcgcactcg gtgtcgtgct gttccccgtc cacaacaatt tccaccacct
     2521 gaacatcgag accctgctgc tggcgctg
//
LOCUS       DBNGZW010000269         2895 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1318221, whole genome shotgun sequence.
ACCESSION   DBNGZW010000269 DBNGZW010000000
VERSION     DBNGZW010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2895)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2895)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2895
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1318221"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2293
                     /gene="pbpC"
                     /locus_tag="AC16JC_06480"
     CDS             <1..2293
                     /gene="pbpC"
                     /locus_tag="AC16JC_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007079695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HIR5164378.1"
                     /translation="AAAAFWLLPRVIPHQPLRARFGTSTAVYDADGALLRLTRAADDK
                     YRVWVPLDRISPLLVDATLLQEDRHFRRHPGVNPVALVRGAWQTYVVRQRREGGSTIT
                     MQLARLLYHIRSSTPGGKLVQIARAVQLELCYTKREILEAYLNLVPYGGNVEGAAAAS
                     LVYFGKDPSQLTLPEALTLAVLPQSPTRRAPTAGRQDAALREARNALYARWQAMHPRV
                     EGEADLMRLAVHMGSPAALPFRAPHLVDAVLADAGAPAEIHTTLDRQLQAVLEREVRA
                     YVAAQARIGVHNAAAVLVDARTMGVKALVGSADFFDAGIAGQVNGTAAKRSPGSTLKP
                     FIYALGLDQGVLHPMTVLKDAPSAFGAYSPENFDGRFVGPLTARDALIRSRNVPAVYV
                     ASQLSKPDLYDFLEMAGVSGLRSREYYGLALVLGGGEVTMEELVSLYGALASRGVVRP
                     VHYRADQTPGAGVQVLSPEASYVTLEMLKDNPRPDAPSAARQGRLSVYWKTGTSYGFR
                     DAWTIGIFGPYILAVWIGNFDGEGNPAFVGVQVAAPLFFRMVDAIEAQQPSLGEPIRR
                     FPANLTLVDVCAGSGDLPNADCPRTVPTWFIPGKSPIRISTLHQALLIDDRTGLRDCP
                     PYTPGPAHRVVYEMWGSDMLRLFQQAGLPRRTPPHFDPRCESHSIGPPGGSPPQITSP
                     LRGVVYSLRAKRIGQETITLRANTGAGVRAAYWFVGNAFLGQVPPGGSLAWKPEASGS
                     FVVRVVDDQGQADSRLVQLAVVP"
     gene            complement(2421..>2895)
                     /locus_tag="AC16JC_06485"
     CDS             complement(2421..>2895)
                     /locus_tag="AC16JC_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164379.1"
                     /translation="WVPWSLDRRHLFRLDGRRSFGRHWSASMAFEAMSGAPITPVDGV
                     LIVGFPDATGHGVTRDSGQGLGPAYIYGKENSARSSGTARLDLAARYAFTGPGHSRMT
                     LGFSVLNVGFGPVAPLRPKEAGFDPSDSGVLRGRVRYERLFNLPAVPSLTLRIEF"
BASE COUNT          451 a          990 c          982 g          472 t
ORIGIN      
        1 cgccgctgcc gcgttttggc tattgccgcg cgtcattcca caccaaccgc tgcgggcccg
       61 tttcgggacg tccacggcgg tgtacgacgc ggacggcgct ctgctccgcc tgacccgcgc
      121 cgccgacgac aagtaccgcg tctgggtgcc gctcgatcgg atatccccgt tgctcgtcga
      181 cgccacgctg ctccaggaag accgccattt ccgccggcat ccgggcgtga atccggtggc
      241 gttagtgcgg ggtgcgtggc agacgtacgt ggtgcggcag cggcgcgagg gcggctcgac
      301 gatcaccatg cagctcgccc gcctgctgta ccacatccgc tcctccaccc cgggcggcaa
      361 actggtgcaa atcgcccgcg cggtccagct ggagctctgc tacacgaagc gggagattct
      421 cgaagcgtat ctcaacctgg tgccctatgg cggcaacgtc gaaggggccg ccgccgcgag
      481 cctcgtctac ttcggcaagg atccgagcca gctgacgttg cccgaggccc tgacgctcgc
      541 cgtcctgccg cagagcccga cgcgccgggc cccgacggcg gggcggcagg acgcggcact
      601 gcgcgaagcg cggaatgctc tgtacgcgcg ctggcaggcg atgcatcccc gcgtcgaggg
      661 cgaagcggat ctgatgcggc tcgccgtcca tatgggatcg ccagccgcgc tgccgttccg
      721 cgcgcctcat ttggtggacg ccgtgctcgc tgacgccggc gcaccggcgg aaatccacac
      781 cacgctggac cggcagctgc aggctgtgct agagcgcgag gtccgcgcct acgtcgccgc
      841 ccaggcccga atcggcgtcc acaatgccgc cgccgtgctg gtggatgcgc ggaccatggg
      901 tgtgaaggcg ctggtggggt cggcggactt cttcgatgcc ggcatcgcgg ggcaggtgaa
      961 cggcaccgcc gccaagcgct cgcccggctc gaccctcaag ccgttcatct acgccctggg
     1021 tctcgatcag ggcgtgctgc acccgatgac cgtactcaag gacgcgccat ccgctttcgg
     1081 ggcctatagc cccgagaact tcgatggccg gttcgtgggc ccgctcacgg cccgggacgc
     1141 gctgatccgc agccgcaacg tgccggcggt gtatgtcgcc tcgcagctgt ccaaaccgga
     1201 tctctacgac ttcctggaga tggccggcgt atcagggctg cgatcgcgcg agtactacgg
     1261 gttggcactg gtgctgggcg gcggcgaggt caccatggag gagctggtct cgctgtacgg
     1321 cgcgctggcc agtcggggcg tcgtgcgacc agtgcactat cgcgccgacc agacgccggg
     1381 ggccggcgtc caggtgttga gccccgaggc gagctacgtg acactcgaga tgctcaagga
     1441 caacccacga cccgatgcgc cgtccgccgc gaggcagggc cggctctccg tgtactggaa
     1501 gaccggcacc tcctacgggt tccgcgatgc ctggaccatc gggatcttcg gtccctacat
     1561 cctggccgtg tggatcggga actttgacgg cgaggggaat cccgcgttcg tcggcgtgca
     1621 ggtggcggcc ccgctctttt tccggatggt ggacgcgatc gaggcccagc agccttcgtt
     1681 gggagagccg atccggaggt tccctgcgaa cctgacactc gtcgatgtct gtgccggctc
     1741 gggggatctg cccaacgccg actgcccccg gacggtgccg acctggttca ttcccggcaa
     1801 atccccgatt cgcatcagca cgctccacca ggcgctcctg atcgacgatc gcaccgggtt
     1861 gagggactgt ccgccctaca cgccggggcc cgcacatcgg gtggtctacg aaatgtgggg
     1921 ctccgacatg ctcaggctgt tccaacaggc ggggctgcca cgccgcaccc cgccgcactt
     1981 cgacccacgg tgcgaatcgc atagcatcgg gcctccgggc ggctccccac cgcagatcac
     2041 ctccccgctc aggggcgtcg tgtattcgct ccgtgccaaa cggattgggc aggagaccat
     2101 caccttgcgg gccaacaccg gcgcgggcgt ccgggcggcg tactggttcg tcgggaatgc
     2161 cttcctggga caggtaccgc cgggggggag cctcgcgtgg aagccggagg cgtcgggttc
     2221 gttcgtggtc cgggtggtgg acgaccaggg tcaggccgat tcccgtctgg ttcagctggc
     2281 ggtcgtgccg tagggtgtat gcgattcctg acggctcaca cgtccctccc agggacgctg
     2341 gacggcaaag cagtcgatct ggtccgaccc tcgcgggtgg ggtgcgggag gaacggcagc
     2401 actaacgcgc acgcccctgc tcagaactcg attcggagcg tcaggctcgg cacggcgggg
     2461 agattgaaca gtcgctcata gcgaacgcgc ccccgcagaa cgccgctgtc ggagggatca
     2521 aagcctgcct ctttggggcg cagcggagcc accgggccga atccgacgtt cagcacggag
     2581 aacccgagtg tcatgcgact gtgtccgggg cccgtgaacg cgtaccgcgc ggcaagatcg
     2641 agtcgagctg tacccgagct cctggcgctg ttctccttcc cgtagatata ggccggtcca
     2701 agaccctgac cagaatcgcg agtcacgccg tggccggtgg catcggggaa tcctacgatc
     2761 aacactccgt cgaccggagt gatcggcgcg ccgctcatcg cctcgaacgc catggaagct
     2821 gaccagtgtc ttccgaatga ccggcgcccg tccaggcgga acaggtgccg tcgatccaga
     2881 gaccacggaa cccaa
//
LOCUS       DBNGZW010000270         3575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_24018, whole genome shotgun sequence.
ACCESSION   DBNGZW010000270 DBNGZW010000000
VERSION     DBNGZW010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3575
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_24018"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..335)
                     /locus_tag="AC16JC_06490"
     CDS             complement(<1..335)
                     /locus_tag="AC16JC_06490"
                     /inference="COORDINATES: protein motif:HMM:NF014606.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HIR5164380.1"
                     /translation="MSKYLVVVLAVLGTFPAARAWSQGSAAAKPATAVVEAPLAPGDA
                     IQLAFWREPQLGGEFPVDENGTVVLPLLGARSVTRLPATQLRQTLMEEYAREIRNQDV
                     RITLLRRVR"
     gene            complement(332..2749)
                     /locus_tag="AC16JC_06495"
     CDS             complement(332..2749)
                     /locus_tag="AC16JC_06495"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GumC family protein"
                     /protein_id="HIR5164381.1"
                     /translation="MSSRLPTLRDAMPPLESRSGRRTGRSLRDTRGRGSEPELPLVGA
                     VLRRRRASILTATFLVLGAVAVLTFFWPRTYTSSTTFLVERPGGQPNSPALDVLERLG
                     SVGQIQTEINLLSSRSVVEPVVENLALHVTVLSDGMEWRPSEVFPEFEAGRESAAGTY
                     RLVRGPAQTYQVRTVPGDSVLAVASPDSLTRFGGIGLRLPESLNATDLAIRVAPFPTA
                     VAQTQGRIAAALVNRESDLIELTCKGSTPLTAQQVCDGISRSYIRLRTDLQRADASAT
                     ATFLRDQVGRLGDQLTAVEDSIAAYSRGHQVVALDERASQETQQDIQVQAQRDQLVAE
                     HDALARLIRGIDATPEGARKYRDLAGYPALLKNDAVTGLLASLTDLENRRVDLALRRT
                     EQNADLAAVDARITDIERQLRAIATSYEQSLALQISSLGETEDELSRRLAEIPGEQIR
                     TARLERERSGIEDVYKMLGTRLQEAEVAQAVQLPSVRVVDPASLPFQASWPRVPLNLG
                     LGLLMGLAAGVFLAVVRELRDSRLHERVEVERTAGLPILTMIPRVRRPGPVLPAFVAS
                     QSPSRRERRAERLPQSAQVVLEAFRALAVDMKFAGRRLNDADPITIAVTSATRGEGKT
                     LTACNLALTRASHGVRTLLIDADMRTNGVARFFRSPTPNYGLSDLLAGSADLSAVCTK
                     LRVSGRDTLGVIPAGTPTPHSAELLESPNMAALLEDARREFDLVVIDTPPLNAITDAA
                     PVAAMVDGVVLVVRGGVTDREALQLTLNRLARVNGRVLGVVFNDVRLPQAYVNRSGAA
                     FAEGRSW"
     gene            complement(2746..>3575)
                     /locus_tag="AC16JC_06500"
     CDS             complement(2746..>3575)
                     /locus_tag="AC16JC_06500"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HIR5164382.1"
                     /translation="GSLGDLESLLMRRVVDEVLIALPIKSCYARIQDVIHTCERLGVQ
                     STYLADIFEPSLGRVRYEQPAFLTVRVVQDDFRLIIKRAIDLVGAALGLVVLAPLLLL
                     IAGLIRLTSPGPVLFAQDRYGLHKRLFRMYKFRTMVSDAERLQPALEAQNEARGPVFK
                     IARDPRVTRVGRFLRRSSMDELPQLWNVLRGDMSLVGPRPLPVRDVGRFDEGWLMRRF
                     SMPPGLTCLWQINGRSEVDFARWVQLDLEYIDRWSLRLDCLILLRTIPAVLHGRGAQ"
BASE COUNT          549 a         1247 c         1264 g          515 t
ORIGIN      
        1 atgcggaccc ggcgcagcag cgtgatgcgc acgtcctggt ttcggatctc ccgcgcgtac
       61 tcttccatca gggtctgccg cagctgtgtc gcgggcagcc gggtcaccga gcgcgcgccg
      121 agcagcggca gcaccaccgt gccgttctcg tccaccggga actcaccgcc cagctgcggc
      181 tcgcgccaga acgccagctg aatggcgtcg cccggggcga ggggcgcctc gacgacggcc
      241 gttgccggct tcgccgccgc ggagccctga ctccatgccc tggccgcggg gaaggtcccc
      301 aggacggcga ggacgaccac caggtacttg ctcaccagga cctcccctcg gcgaacgcgg
      361 cgccggagcg attgacgtat gcctgcggca gtcgcacatc gttgaacacc accccgagca
      421 ctcggccgtt cacgcgggcg aggcggttca gggtcaattg cagggcttcg cgatcggtca
      481 ccccgccgcg caccacgagc acgacgccgt cgaccatggc ggcgactggc gccgcgtcgg
      541 tgatggcgtt gaggggtggc gtatcgatca cgaccagatc gaactcccgc ctggcgtcct
      601 cgagcagcgc cgccatgttc ggggattcga gcagctcggc cgaatgtggg gtgggggtgc
      661 cggcgggaat gacccccagc gtgtcccggc cgctcacccg gagtttcgtg cacacggccg
      721 agaggtccgc cgagccggcg agcagatcgc tcagcccgta attgggtgtg ggagaccgga
      781 agaagcgcgc gacaccgttc gtccgcatgt cggcatcgat gaggagcgtc cggacgccgt
      841 gactggcccg cgtcagcgcg aggttgcagg ccgtcagcgt cttgccttca ccccgcgtgg
      901 cgctcgtcac ggcgatggtg attggatcgg catcgttcag gcgccgaccg gcgaacttca
      961 tgtcgacggc gagcgcacgg aacgcctcca gcaccacctg ggcgctctgc ggcagccgtt
     1021 ccgcccggcg ctcccgtctg gatggcgact gggacgccac aaaggccggc agcacgggtc
     1081 cgggacggcg gaccctcggg atcatggtga gaatcgggag gccggcggtc cgctccacct
     1141 cgactcgctc gtgcaggcgc gaatcccgca gctcgcggac gacggccagg aacacgccgg
     1201 ccgccaaccc catcaggagg ccaagtccga ggttgagcgg caccctgggc cacgacgcct
     1261 ggaacggcag ggaggcgggg tcgacgacgc gcacgctcgg gagctggacc gcctgcgcga
     1321 cttccgcctc ctgcagccgc gtcccgagca ttttgtatac gtcctcgatg cccgaccgct
     1381 cgcgctccag acgggcggtc cggatctgct cgccggggat ctccgccagg cggcgactca
     1441 actcgtcctc cgtctcgccg agcgagctga tctggagcgc gagcgactgc tcgtagctcg
     1501 tcgcgatggc gcgcagctgc cgctcgatgt cggtgatccg cgcatcgacc gcggcgaggt
     1561 ccgcattctg ctccgtgcgc cgcaacgcga ggtccacccg ccggttctcg agatcggtga
     1621 ggctcgcgag cagcccggtg acggcatcgt tcttcaggag cgcgggatac ccggcgaggt
     1681 cgcggtactt ccgcgccccc tcgggtgtgg cgtcgatgcc gcggatcagg cgggcgaggg
     1741 cgtcgtgctc cgccacgagc tgatcgcgct gcgcctgcac ctggatgtcc tgttgcgtct
     1801 cctgcgaggc gcgctcgtcg agggcaacga cctgatgccc gcgggagtag gccgcgatgc
     1861 tgtcttccac ggcggtgagc tggtcgccca gccgacccac ctgatcccgc aggaacgtcg
     1921 cggtggccga ggcgtccgcg cgctgcaggt cggtccgcag gcggatgtag ctgcgcgaga
     1981 tgccgtcgca cacctgctgc gcggtgagcg gcgtggaccc cttgcaggtg agctcgatca
     2041 ggtccgattc acgattgacg agcgcggcgg cgatgcggcc ctgcgtctgg gccacggccg
     2101 tggggaacgg cgcgacgcgg atggccaggt cggtcgcgtt cagggattcc ggcagacgga
     2161 gcccgatccc tccgaaccgg gtgagggaat ccggggacgc cacggcgagg acgctgtcgc
     2221 cgggaacggt gcgtacctgg tatgtctgcg ccgggccgcg caccagccgg taggtcccgg
     2281 ccgccgactc gcgacccgcc tcgaactcgg ggaagacctc gctcgggcgc cactccatgc
     2341 cgtcggacag gaccgtcacg tggagcgcga gattctcgac gaccggctcc accacgctgc
     2401 ggctgctcag cagattgatc tcggtctgga tctggccgac ggagccaagt cgttccagca
     2461 cgtcgagcgc cggggagttg ggttgcccgc ccggccgctc gaccaggaac gtcgtggacg
     2521 aggtgtaggt ccggggccag aagaacgtca gcaccgccac ggcccccagg acgaggaacg
     2581 tagcggtcaa gatgctcgcg cgccgccgcc gcagcacggc gcctacgaga ggcagctccg
     2641 gctcggagcc cctcccccgg gtgtcgcgca gcgaccggcc ggtccgccgc ccggatcggc
     2701 tctcgagcgg cggcatcgcg tcgcgcaacg tgggaagccg actgctcatt gcgcccccct
     2761 gccgtggagc acggccggga tcgtcctgag gaggatcagg cagtcgagtc gcagggacca
     2821 gcggtcgatg tactcgaggt cgagctgcac ccagcgcgcg aagtccacct cgctgcgtcc
     2881 gttgatctgc cacaggcagg tgagccccgg gggcatgctg aagcggcgca tcagccatcc
     2941 ctcgtcgaac cggccgacat cccgcacggg gagtggacgt gggccgacca gggacatgtc
     3001 gcccctgagt acgttccaca gttgcggcag ctcgtccatc gagctgcgcc gcaggaagcg
     3061 cccgacgcgg gtgacgcgcg ggtcgcgggc gatcttgaag accgggcccc gggcctcgtt
     3121 ctgcgcttcc agggccggct gcaggcgctc cgcgtccgac accatcgtgc ggaacttgta
     3181 catccggaac agccgcttgt ggagcccgta gcgatcctgg gcgaacagca ccggacctgg
     3241 actcgtgagc cggatgagtc cggcgatcag gaggagcagc ggggccagca ccacgaggcc
     3301 gagcgccgcc cccacgaggt cgatggcccg cttgatgatc agccggaagt cgtcctgcac
     3361 caccctgacc gtgaggaagg ccggctgctc gtaccgcacc cgccccagcg acggctcgaa
     3421 gatgtccgcc aggtaggtgg actggacgcc cagtcgctca caggtgtgaa tgacgtcctg
     3481 aatccgcgcg tagcacgatt tgatcggcag ggcgatgagc acctcgtcca ccacccggcg
     3541 catcagcagc gattccagat cgccgagcga gccaa
//
LOCUS       DBNGZW010000271         1893 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1115338, whole genome shotgun sequence.
ACCESSION   DBNGZW010000271 DBNGZW010000000
VERSION     DBNGZW010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1115338"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="AC16JC_06505"
     CDS             complement(<1..297)
                     /locus_tag="AC16JC_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018189005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5164383.1"
                     /translation="MITKLNVTSVKVLDQEEALDFYVNKLGLEKGNDVRQGPFRWLTV
                     RVPGDTGTEIFLEEPGPPLHDDATAAQLRELITKGAMSSLVFNTDDVRGLYETLQ"
     gene            complement(339..791)
                     /locus_tag="AC16JC_06510"
     CDS             complement(339..791)
                     /locus_tag="AC16JC_06510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5164384.1"
                     /translation="MSRDVEDLNRRLLRARDAMDRSYAEPLDVRALAVIAHMSEAYFI
                     RSFRATFGETPHRYLQRRRVERSMFLLRETERSITDICVDVGFSSLGTFSRTFHEIVG
                     ETPSEYRLGHEPMPAPGCVQMAGTRPVAAPRAAARSGSFGEADAPAAP"
     gene            1321..1566
                     /locus_tag="AC16JC_06515"
     CDS             1321..1566
                     /locus_tag="AC16JC_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164385.1"
                     /translation="MTTDANGAYTAKVSAWTLEALADSSSSQLSVFVTPPAGMQITAV
                     TQASTVPVGPIAPNVPEWYFMLERDLNGPIDITLAPR"
     gene            complement(1601..1677)
                     /locus_tag="AC16JC_06520"
     tRNA            complement(1601..1677)
                     /locus_tag="AC16JC_06520"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1641..1643),aa:Glu,seq:ctc)
BASE COUNT          281 a          664 c          637 g          311 t
ORIGIN      
        1 ctgaagggtc tcgtacagcc cacggacgtc atccgtattg aagacgagcg agctcatcgc
       61 gcccttcgtg atcagctcgc gcagctgcgc ggcggtggcg tcgtcgtgca ggggcgggcc
      121 cggctcctcc aggaagatct cggtgccggt gtcgccgggg acgcgcacgg tgagccagcg
      181 gaagggaccc tgccgcacat cgttgccctt ctcgaggccc agcttgttga cgtagaagtc
      241 gagcgcctcc tcctgatcga gcaccttgac ggaggtgacg ttgagcttgg tgatcatcgt
      301 ttgctctcct ctgcgacgtg atgtgaccgg cgctgacgct agggcgccgc aggcgcgtcc
      361 gcttctccga aactgcccga tcgcgcagcc gcccgcggcg cggcgaccgg cctcgtgccg
      421 gccatttgga cgcagcctgg tgccggcatc ggctcgtggc ccaggcggta ctcggatgga
      481 gtctcgccga cgatctcgtg gaacgtgcgg ctgaaggtcc cgaggctgct gaagccgacg
      541 tcgacgcaga tgtcggtgat gctccgttcg gtctcgcgga gcaggaacat cgagcgctcg
      601 acgcgccggc gctgcaggta acggtgcgga gtctccccga acgtcgcgcg gaagctgcgg
      661 atgaagtacg cctcggacat gtgcgcgatc acggccagag cgcggacgtc gagcggctcg
      721 gcgtacgagc ggtccatcgc gtcgcgcgcg cgcagcaggc ggcggttgag gtcctcgacg
      781 tcccggctca ccccgtaaac gctaggccat cgccgcagga gcgccagcgg ataccgctcg
      841 gccgattgtt cttcccgtta cagagcggaa cgggcgaacg ggcgccggcg tcctcatgga
      901 caagagcact gttgccgccg ttggcggccg cgaggagcgc gttccatgtc aaagacacga
      961 cttgcggcgc tggccgtgac cctggccttc gccggtgccg cctgcgggag cgccacgtca
     1021 gcgtcgccgt cgccgtccag cgcggccacg ccaccctcga gcagcatccc ctcacccacc
     1081 gcgacgccgt cgagcgcgcc aactgcgctt gggacacggc cggcgtcgac ggctactccg
     1141 acgccaacat cctcgcctcg ccccacggag ccgacaccgg tcgtactcgc cgtcagcggt
     1201 cgcgtgtcgc tggcgtctga cggccgtccc gtcccgggtg tccgtctgcg cttcaacgcc
     1261 gccgccatca acggtggctg cccgaccgcg tgcccgaagg cacctggtcc cgacgtcgtc
     1321 gtgaccactg acgccaacgg tgcgtacacc gcgaaggtgt cggcatggac cctcgaggcg
     1381 ctcgcagatt cgtcctcatc gcagctcagc gttttcgtca cgccgcccgc cgggatgcag
     1441 atcaccgcgg tgacgcaggc gagcacggtg ccggtcggcc cgatcgcgcc gaacgtgccc
     1501 gagtggtact tcatgctcga gcgcgatctg aacggcccga tcgacatcac gctcgcgccg
     1561 cggtagcgcg gtgcggtcgt tgcgggttcg cgactggcgc tggtagcccg tagcggattc
     1621 gaaccgctga tctcagcctt gagagggctg cgtcctgggc ctctagacga acgggccggg
     1681 agaacaccat tgtaccggtg atgcccctgt ctggtcattc acacacgtga gcgtcgctgt
     1741 catcgcgccg ggagtccgcc gcatcccgat ccccctcccg atcccggccc tgaagatcat
     1801 caacgcgtac ctcgtcgagg gcgagcgcgg ctggtccctg gtcgattcgg gcatccatac
     1861 gcccgaggct gagaagacgc tccgcgatgc gct
//
LOCUS       DBNGZW010000272         4073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_365720, whole genome shotgun sequence.
ACCESSION   DBNGZW010000272 DBNGZW010000000
VERSION     DBNGZW010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_365720"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..837
                     /locus_tag="AC16JC_06525"
     CDS             <1..837
                     /locus_tag="AC16JC_06525"
                     /inference="COORDINATES: protein motif:HMM:NF016777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5164386.1"
                     /translation="AEGIDSVLLYPTIGICWEGHVTDPGLATAYTRAYNRWLVEFCQA
                     DRTRLHPVAHISLLDPEGAVEETVRARADGCVGVFLSPDVAARAGRHFDDPAFARFWA
                     TVQDLEMPIAFHVVVRDRQWFRPWQRYDPSDGLFGFAFLAIDVMAAFTQMLASGMFER
                     YPRLKCAVLEAGSNWIASWLDRLDHKHRVMAHRTPIRMAPSEYFYRQCLISADPDESL
                     TAQMVEHLGADYFIWASDYPHIDASMGVVRELRERLAPLSAEARRKVLGDNAVRFYKL
                     TG"
     gene            1431..2753
                     /locus_tag="AC16JC_06530"
     CDS             1431..2753
                     /locus_tag="AC16JC_06530"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5164387.1"
                     /translation="MTARTGPGARVTWRARVADPMMGAVLALGVTQITAWGTSYYCLG
                     VLAGPISHDTGWSRGFVFLGFTVALLAMGLVSGTVGRAIDRHGGRAVMTLGTVLVSAG
                     LFALAHVRSEPAYLAVWAFLGLGMRLCLYDAAFAALVQVAPSRGRTAISYLTLFGAFA
                     SSVFWVVGHALNEQVGWRQTLVLFALINLVVCLPLHWLGLAQRESPEAVAAPLGGAAA
                     SPDGPPLEGRARSVALVLFALIMSLNGFVFGVISVQLVPLLEAAGLATAAAVWVASTK
                     GVAQFGGRVVEILFARNLRAITVGRIAVGILPPSLLLLLFGTGSLPMVVAFTLLMGAS
                     QGVITIVRGAVPLALFGVKGYGAVLGVIAMPVLIVNAASPTAFAWIVDRWGWGTARVF
                     LLVGCSAAWLAMELMSRWYERQRGSVAGGTHQRAARAAGTPRERLRGG"
     gene            complement(2844..3071)
                     /locus_tag="AC16JC_06535"
     CDS             complement(2844..3071)
                     /locus_tag="AC16JC_06535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164388.1"
                     /translation="MDLASQRIRDKLRYGILPSCEPVMTWAGFGNGTLCDACDISIQP
                     KEVEYEVELENGQTLHLHVACVGLWQVLRTD"
     gene            complement(3732..>4073)
                     /locus_tag="AC16JC_06540"
     CDS             complement(3732..>4073)
                     /locus_tag="AC16JC_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164389.1"
                     /translation="VAGCAGDFEGNGSRGYVVLLRRDSDGHYVPHVFLARDRTFHVVE
                     LEWHATDDSAWVGPFCRAKPKAGTFQAPDFEGTGERVWIPVVGDLFTVGWWTYYWRPD
                     LQRFDAILTTD"
BASE COUNT          556 a         1452 c         1350 g          715 t
ORIGIN      
        1 gccgagggca tcgacagcgt cctcctgtat cccaccatcg gcatctgctg ggaggggcac
       61 gtcaccgacc ccggcctggc caccgcctac acgcgggcct acaaccgatg gctggtcgag
      121 ttctgccagg ccgatcgcac gcgcctgcac ccggtggcgc acatctccct cctcgacccc
      181 gagggcgcgg tggaagagac ggtccgggcg cgcgccgacg gctgcgtggg cgtcttcctc
      241 tcgccggacg tcgccgcgcg tgccggccgc cacttcgacg atcccgcatt cgcccgcttc
      301 tgggcgacgg tgcaggatct cgagatgccg atcgcgttcc acgtggtggt acgcgaccgc
      361 cagtggttcc gcccgtggca gcggtacgat ccctcggacg gcctgttcgg cttcgccttc
      421 ctcgccatcg acgtgatggc cgcgttcacg cagatgctcg cctccggcat gttcgagcgc
      481 tacccgcgtc tcaagtgcgc ggtcctggag gcggggtcga actggatcgc ctcctggctc
      541 gaccggctcg accacaagca tcgcgtgatg gcccaccgga cgccgatccg catggcgccc
      601 agcgagtact tctaccgcca gtgcctgatc tcggccgacc cggacgagtc cctgaccgcc
      661 cagatggtcg agcacctggg ggccgactac ttcatctggg cctcggacta tccccacatc
      721 gacgcatcga tgggcgtggt ccgcgagctg cgagagcgcc tcgcgccact ctccgccgag
      781 gcgcgccgga aggtgctggg cgacaacgcg gttcgcttct acaagctgac cgggtgacgc
      841 cgcaggccaa ggtgccggtg aatcccggcc atcttccgtt ggccgggacg ctcagtcgtt
      901 cgcaggagcc cgatcctctt gctcttgccg gaggcccgct gcacgtggca gcgagccgtt
      961 ggcggtgaga acggcggcca ccacgacgga acactgcggg ttgccgcacc cgcagcccag
     1021 ggcgtcgcag gcgatgacgg ccagggtgtc gttcatcgcg ctgtagcggg cgaaagtccg
     1081 ctcgatcttt cgacaacgcg cacacgcggc ggcggtccgg aaccaatgca ggtgcgcgaa
     1141 cgaccgcttc cgtagccgtg ctggcgctcg gcgcgatatc ccctgccatc gtcgcctcat
     1201 ctcgttcgag gtctcctatc gcggcggctg ctctccgggg cccgtgggcg ccgaggtccg
     1261 aggagcaagg actatgccgc gtcaggaagc tatggccgac gaatgcttgg gctcgaccga
     1321 cccgccaccg ctgtaaattg aacctggtaa gaactaccgg agcatcactc gtcacggtcg
     1381 gagtagatcc cgtgatagtc tcgcgccatg ccgccgcgcc cgctcgttcc gtgaccgcac
     1441 gcactggacc gggcgcgcgt gtcacctggc gggcgcgggt cgccgacccg atgatgggcg
     1501 cggtgctcgc gctcggcgtc actcagatca ccgcctgggg aacgagctac tactgtctcg
     1561 gcgtgctggc ggggccgatc agccacgaca ccgggtggag ccggggcttc gtcttcctcg
     1621 gcttcaccgt cgcgctgctc gccatggggc tcgtctcggg caccgtgggt cgtgccatcg
     1681 accgacacgg cgggcgcgcg gtcatgacgc tcggcaccgt cctcgtgtcc gcgggcctgt
     1741 tcgcgctcgc ccacgtccgc agcgagccgg cgtacctggc ggtctgggcg ttcctcggcc
     1801 tcggcatgcg gctgtgcctc tacgacgccg cgttcgccgc gctggtgcag gtcgctccgt
     1861 cgcgcgggcg cacggcgatc tcctacctca ccttgttcgg agcgttcgcc tcgtcggtgt
     1921 tctgggtggt cggccacgcc ctcaacgagc aggtcggctg gcgacagacc ctcgtcctgt
     1981 tcgccctcat caatctggtg gtgtgcttgc cgctgcactg gctcggcctc gcccagcgcg
     2041 agtctcccga ggcggtggcc gccccgttgg gcggtgccgc ggcgtccccg gacggccccc
     2101 cgctcgaggg gcgcgcccgc tcggtcgccc tcgtgctctt cgccctcatc atgtcgctca
     2161 acgggttcgt gttcggcgtc atctcggtgc agctcgtccc gctgctcgaa gccgcgggcc
     2221 tggcgacggc cgccgcggtc tgggtcgcct cgacgaaggg cgtcgcccag ttcgggggac
     2281 gggtggtcga gatcctgttc gcccgcaatc tgcgcgcgat caccgtgggc cgcatcgcgg
     2341 tcggaatcct gccgccgtcc ctgctcttgc tgctgttcgg caccgggagc ctgccgatgg
     2401 tcgtcgcgtt caccctgctc atgggcgcgt cccagggcgt catcaccatc gtccggggtg
     2461 cggtgcccct cgcgctgttc ggggtaaagg gatacggagc cgtgctgggc gtcatcgcca
     2521 tgccggtgct catcgtcaat gccgcctccc ccaccgcctt cgcgtggatc gtggaccgct
     2581 ggggctgggg gacggcgcgc gtgttccttc tggtgggctg ctcggccgcg tggctcgcca
     2641 tggagctgat gtcgcgctgg tacgagcggc agcgcgggtc cgtcgccggc gggacgcacc
     2701 aacgcgcggc gcgggcggcc ggcactccgc gggaacgtct ccgcggcggg tagaccgctc
     2761 cgtccaggta gagctcgccg accggcgagc gttggcgaag ggcccgacga acgtatgtcg
     2821 ccgagcgtag cctccggcaa gccctagtcg gtccgcaaca cctgccacag gcccacgcac
     2881 gcgacatgca agtggagcgt ctggccgttc tcgagctcga cctcgtactc cacttccttc
     2941 ggctggatgc ttatgtcgca cgcgtcgcac agagtcccgt tcccgaaccc cgcccaggtc
     3001 atgacgggct cgcaactggg caggatgccg taccggagct tgtcgcgaat gcgttgcgag
     3061 gcgaggtcca taggcgcacc ccgttccctt ctgcttgtct gtccccggta cttaccgcgg
     3121 tgaattgcat ggggcgagcc agtaatctcg cgaccacgaa atcggggctt tggtctgacg
     3181 gccacgcggc gatgcgacca atactgggtc atggtcgcct cagtggggcg ggaagctcgg
     3241 agactctatc tgcgcggcgg tgggctctga cgccccgggc aatgacgagc gtccgtcgat
     3301 ggaggctctt gcgcgagacg ctcggctgag gtactgtgcg cccgccgcca tcctggtctt
     3361 cgtcggccgt cgggacccgg gtgcgctggc tcggcctcct tcgctcctgg taaagcgagg
     3421 ttgcgtcggt ctccgcacca ccttcccgtg gatgttgtcg taccgccccg ctactcaagg
     3481 ggggttcccg ccatgctgac caacgacgag ctccggcaac gcgtgcagcg ctcgctcacg
     3541 agtggcgcac tgttccccgc gcctcaaacc gcgtgggcgt tgctacacga tctggcgagc
     3601 cgaatcggag gcggcatccg tccgtgacgc ctgacttctt gaccagttat cgtcgcggtg
     3661 cggcgtgcct tcgttgacga acacaagcgc ggcgcccccc ccactcctgc cgcaacctcg
     3721 cgactcccgg atcaatccgt cgtcaagatc gcatcaaatc gctggaggtc gggacgccag
     3781 tagtaggtcc accacccgac cgtgaacaga tcaccgacga ccggaatcca cactctctcc
     3841 ccggtccctt cgaaatccgg cgcctgaaag gtcccggcct tcggcttcgc tcggcaaaag
     3901 gggccaaccc aggccgagtc atcggtagcg tgccactcca gctccacgac gtggaatgtg
     3961 cggtcacggg ccagaaacac gtggggaacg tagtgcccat cgctgtcccg cctgagaaga
     4021 acgacgtagc ctcgcgagcc gttgccttcg aagtcgccgg cacatcccgc gac
//
LOCUS       DBNGZW010000273         4644 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_910283, whole genome shotgun sequence.
ACCESSION   DBNGZW010000273 DBNGZW010000000
VERSION     DBNGZW010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4644)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4644)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4644
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_910283"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1186)
                     /gene="hutI"
                     /locus_tag="AC16JC_06545"
     CDS             complement(<1..1186)
                     /gene="hutI"
                     /locus_tag="AC16JC_06545"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018086094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HIR5164390.1"
                     /translation="MVTCRGPGRARRGRELSELEVLRDAAVLIEGERIAWVGPRRDAP
                     RSGSGVEVVEVSGTLFPGFIDCHTHAVFGAPRLEDHERRALGESYQAIAAAGGGILQS
                     VRDVRARSATELERLARNRLLTLRDHGSTTIEVKSGYGLELESELKQLRIVHRLASEG
                     LQLVPTFLGAHEVPPEYRERRAEYVRLLVDEMLPAVIREDLARCCDVFCEPAVFSVDE
                     TRMILTAARRAGLALKLHADELQGAGGAELAAELGALSADHLASISDAGVRALAASET
                     VAVLLPGTMSFLGKARQAPARRLVEAGVAIALATDFNPGSSPTIGLPLVMSLAVSQLG
                     LRHTEAVLAATVNAAAALGLAADRGQIAPGFAADLVAADVSDWREIAYWVGANLVTAV
                     FTG"
     gene            complement(1213..2877)
                     /gene="hutU"
                     /locus_tag="AC16JC_06550"
     CDS             complement(1213..2877)
                     /gene="hutU"
                     /locus_tag="AC16JC_06550"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HIR5164391.1"
                     /translation="MSGARAVRAPRGSERSCRGWVQEAALRMLMNNLDAEVAERPDEL
                     VVYGGTGRAVRSWAAFDAIVRTLRTLGDDETLLVQSGKPVGVFATHPDAPRVLIANAN
                     LVGRWATWDVFRDLERRGLLMYGQMTAGSWIYIGTQGILQGTYETFGAVARRHFGGSL
                     AGRVVLTGGLGGMGGAQPLAATMNDGVCLAVEVDPTRVQRRLETRYLDKATASLEEAL
                     EWCEGARRSRSPLSIGLIGNCAEVVPELVRRGFVPDAVTDQTSAHDTLHGYVPAGLPL
                     AAALALRAADPAEYIRRAEQSIASHVRAMLALQRAGAVAFDYGNNIRTVAADAGVTDA
                     FEIPGFVPEYIRPLFCEGKGPFRWVALSGDPADLLRTDRLVLELFPDNQHLARWIRMA
                     QERVAFQGLPARICWLGQGERARFGVALNDLVARGELSAPIVIGRDHLDTGSVASPFR
                     ETEGMRDGSDAIADWPILNALLNTASGASWVSFHHGGGVGIGNSLHAGQVTVADGTAA
                     ARRRLERVLTNDPGIGVVRHADAGYPEAVATARREGLRLPMLEAGD"
     gene            complement(2874..4367)
                     /gene="hutH"
                     /locus_tag="AC16JC_06555"
     CDS             complement(2874..4367)
                     /gene="hutH"
                     /locus_tag="AC16JC_06555"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011522214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HIR5164392.1"
                     /translation="MAPTVTLDGHSLSIGDVVAVARHGARVAVAPDTRARVAHSRAGI
                     EAALARGETIYGVNTGFGKLAHVRIPPDQLRALQLNLIRSHASGVGDPLAPEAVRAMM
                     LLRANVLLMGTSGVRAALPDTLVAMLNAGIHPTVPSQGSVGASGDLAPLAHLALALIG
                     EGPDGARLAAAGIQPVTLEAKEGIAFVNGTQAQTGLAALLVYDAWVLWRTAHGAAAMS
                     LEAVRGTPVPFDARIHDVRPHPWQQRSAALLRALLADSEIRESHRHGDSRVQDAYSLR
                     CTPQVLGAVGEGVAFAERLVATELNAATDNPLVFGPDVLSGGNFHGQPIALALDVLGI
                     VLATLAGLAERRLERVVNPDLSSGLPAFLAHDPGLDSGFMTAQIAAAALVADSRVLAT
                     PASVQSVPTEGNQEDIVPMGMTAAWKAQRILANAVRVVAIELLAGAQGLEYLKPLRPG
                     RGVARLHAAVRELVPPLDADRPMADEIERVAAGVRAGAFDLVSESAL"
BASE COUNT          716 a         1725 c         1581 g          622 t
ORIGIN      
        1 cccctgtgaa gacggcggtt acgaggttcg ccccgaccca gtaggcgatc tcccgccaat
       61 cggacacgtc ggcggcgacg aggtccgcgg cgaagcccgg cgcgatctgt ccgcggtccg
      121 ccgccagccc cagcgcggcg gctgcgttca ccgtggcggc gagcaccgct tccgtatggc
      181 gcaggccgag ctgagagacg gcaaggctca tgacgagcgg cagaccgatc gtcggggagc
      241 tgccggggtt gaagtccgtc gccagcgcga tggccacgcc ggcctccacc agacgccgcg
      301 ccggggcctg gcgcgccttg ccgagaaagc tcatggtccc ggggagcagc acggcgaccg
      361 tctcgcttgc tgccagggcg cggaccccgg cgtcggagat gctcgccagg tggtcggccg
      421 agagcgcgcc gagctccgct gccagctcgg caccgccagc gccttgcagc tcgtccgcat
      481 ggagtttgag cgcgaggccg gcgcgccgcg ccgccgtcag gatcatccgg gtctcatcca
      541 cgctgaacac agcgggctcg cagaacacat cgcagcagcg cgccaggtct tcacggatca
      601 ccgcggggag catctcgtcg acgagcaggc gcacgtactc ggcgcggcgc tcccggtact
      661 cgggcggcac ctcgtgcgcg ccaaggaacg tcggcaccag ctggagacct tccgaggcga
      721 ggcgatggac gatgcggagc tgcttcagct cgctttcgag ctcgaggccg tagccggact
      781 tcacctcgat cgtcgtgctg ccgtggtccc gcagcgtcaa cagccggttg cgggccaggc
      841 gctcgagctc cgtcgccgaa cgcgcgcgca catcccgcac cgactggaga atgcccccac
      901 ccgccgcggc gatcgcctga taactctccc ccagcgcacg gcgctcgtga tcttccagac
      961 gcggcgcgcc gaaaacggcg tgtgtgtgac agtcgatgaa gcccggaaac agggtccccg
     1021 acacttccac cacttcgacc ccggagccgg accgcggcgc gtcgcggcgc gggcccaccc
     1081 acgcaatgcg ttccccctcg atcagcacgg cggcgtcgcg cagcacttcg agctccgaca
     1141 gctcgcgacc gcggcgggca cgtcccggcc cgcggcacgt gaccacggct cgcgcgccga
     1201 cgagcaacgt cctcagtcgc ccgcctccag catcggcagg cgcagccctt cgcgccgagc
     1261 ggtggcgacg gcttccgggt acccggcgtc cgcatggcgc accacaccga tgcccggatc
     1321 gttggtgagc acgcgctcca gccgccgccg cgccgcggcc gttccgtcgg cgaccgtcac
     1381 ctgcccggcg tgcagcgagt tgccgatgcc gaccccccca ccgtgatgga aggacaccca
     1441 cgacgccccg gaagcggtgt tgagcagggc gttgaggatc ggccagtcgg cgatcgcatc
     1501 ggacccgtcg cgcatgcctt ccgtctcgcg aaacggcgac gcgacgctgc ctgtgtccag
     1561 gtggtcgcgt ccgatgacga tcggcgccga cagctctccg cgcgccacca gatcgttcag
     1621 cgcgaccccg aagcgcgcgc gctccccctg ccccaaccag cagatccggg cggggagtcc
     1681 ctggaacgcc acgcgctcct gcgccatccg gatccagcgc gcgaggtgct ggttgtcggg
     1741 gaacaactcc agcacgagac ggtcggtgcg cagcagatcg gccgggtcgc ccgacagggc
     1801 cacccagcgg aacggcccct ttccctcgca gaacaacggc cggatgtact ccggaacgaa
     1861 ccccgggatc tcgaaggcgt cggtgacgcc ggcgtcggca gccaccgtcc ggatgttgtt
     1921 cccgtaatcg aaggccacgg cgccggcccg ctgcagcgcg agcatggccc gcacgtgcga
     1981 cgcgatactc tgctcggcgc ggcggatgta ctcggcggga tcggctgcgc gcaacgcgag
     2041 cgccgcggcg agcgggagtc cagccgggac gtagccgtgc agcgtgtcgt gcgccgacgt
     2101 ctgatcggtg accgcatcgg gaacgaatcc ccgacggacc agctcgggaa caacctccgc
     2161 gcagttcccg atcagcccga tcgacagggg cgagcgactg cgccgcgcac cctcacacca
     2221 ttcgagcgcc tcctccaatg acgccgtcgc cttgtccaga tagcgcgttt cgaggcggcg
     2281 ctgcacgcgt gtgggatcga cctccaccgc caggcacacg ccatcgttca tcgtcgcggc
     2341 gagcggctgc gcccctccca taccacccaa cccgcccgtc agcacgacgc gaccggcgag
     2401 actgccaccg aaatggcggc gcgccacagc gccgaacgtc tcgtaggtgc cctggaggat
     2461 tccctgcgtc ccgatgtaga tccatgaccc cgccgtcatc tggccgtaca tcagcaagcc
     2521 gcgtcgctcg aggtcacgga atacgtccca ggtcgcccac cggccgacga gattggcgtt
     2581 cgcgatcaac acccgcgggg catcgggatg cgtggcgaac acgcccaccg gctttcccga
     2641 ctggaccagc agcgtttcgt cgtcccccag cgtgcgcaac gtccgcacga tggcgtcgaa
     2701 ggccgcccag ctgcgcaccg cccgccccgt gccgccgtac acgaccagct cgtcggggcg
     2761 ctcggctacc tccgcgtcga ggttgttcat gagcatgcgg agggcggctt cctgcaccca
     2821 accgcggcag gaccgctcgc tgccccgtgg cgcccgtacc gcgcgcgcgc cgctcatagc
     2881 gcgctctcgg agacgagatc gaacgccccc gctcgaacgc ccgccgccac ccgctcgatc
     2941 tcgtccgcca tgggtcgatc ggcgtcgagc ggcggcacca gctcccgcac ggccgcgtgc
     3001 agccgcgcca caccgcgccc gggtcgcaac ggcttcaggt attccagacc ctgtgccccc
     3061 gccagcagct cgatcgcgac gacgcgcacc gcattggcga ggatgcgctg cgctttccat
     3121 gcggcggtca tccccatggg cacgatgtct tcctgattcc cttcggtggg caccgactgc
     3181 acactcgccg gcgtggcgag cacccgtgaa tccgcgacca gggccgccgc agcgatctgg
     3241 gccgtcatga atccggaatc gaggcccgga tcatgggcga gaaacgccgg caaacccgac
     3301 gacagatcgg ggttcaccac gcgctccagc cggcgctcgg ccaggcccgc gagcgtcgcg
     3361 agcacgatcc cgagtacgtc gagcgccagg gcgatcggct gtccgtggaa gttgccgccg
     3421 gagagcacgt cgggaccgaa caccagcggg ttgtcggtcg ccgcattcag ctccgtcgcg
     3481 acgagccgtt cggcgaaggc cactccttcg ccgacggcgc ccaggacctg aggggtgcag
     3541 cgcagcgagt aggcatcctg cacccgcgaa tcgccgtgac ggtgcgattc ccggatttcg
     3601 ctgtcggcga gcaaggcccg gaggagcgcg gccgagcgtt gttgccaggg gtgcgggcgc
     3661 acgtcgtgga tccgcgcgtc gaaggggacg ggcgtgccgc gtaccgcttc cagactcatc
     3721 gcggccgcgc cgtgcgcggt gcgccacagg acccacgcgt cgtacacgag caaggccgcc
     3781 aagccggtct gggcctgagt gccgttgacg aacgcgatgc cttccttcgc ctcgagcgtg
     3841 accggctgga tgcccgccgc cgcgagccgc gcgccgtccg gtccttcccc gatgagggcc
     3901 agcgcgaggt gggcaagcgg cgccaggtcg ccggaggcgc ccacgctccc ctgagacggg
     3961 accgtgggat ggatgcccgc gttgagcatc gccacgagcg tatcggggag cgccgcccgc
     4021 acgccgctcg tccccatgag gagcacgttg gcgcgcaaca gcatcatcgc gcgcaccgcc
     4081 tccggcgcca gcgggtcgcc caccccggaa gcgtgactgc gaatcagatt gagctgcagg
     4141 gcgcgcagtt ggtcgggggg gatgcgtacg tgggcgagtt tgccgaagcc ggtgttgacg
     4201 ccgtagatgg tctcgccgcg cgccagcgcc gcctcgatgc cggcacgcga gtgcgcgacg
     4261 cgcgcccggg tatcgggcgc cacagccacc cgggcgccgt gtcgcgcaac cgcgacgacg
     4321 tcgccgatgg acagggaatg accgtcaagc gtgaccgtgg gggccatcgg tggcaggaag
     4381 gtaggcgcgg gcggtgggga cgccaagtag cacgaaaggg ccggcgatgc gtgtcttccg
     4441 caccgccggc ccgcctcgct ttacgcgcgg cagccgcccc tatcgcgccc caggcacctc
     4501 gatgccggtc tcgactgcgg tgggcaggtt ggcgtgcacc gccagctccg gattccccgg
     4561 atcggtctgc agactccagt cgaagatgag atagtcacgt cccacatggg tgacccgcag
     4621 cccgccgtag tggagcgtgc tgcc
//
LOCUS       DBNGZW010000274         5406 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_161423, whole genome shotgun sequence.
ACCESSION   DBNGZW010000274 DBNGZW010000000
VERSION     DBNGZW010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_161423"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..773)
                     /locus_tag="AC16JC_06560"
     CDS             complement(<1..773)
                     /locus_tag="AC16JC_06560"
                     /inference="COORDINATES: protein motif:HMM:NF014896.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIR5164393.1"
                     /translation="MPRPADTLPDPAAAPGVVPRLRYPAAQRGDQVDDYHGTRVADPY
                     RWLEDVDAPDTRAWIDAQNALTLEFLAGVPEREAIRRRLTAIWDYPRYGTPFKKRGQY
                     FFFKNDGLQNQAVLYRQPSRDAEPSVLLDPNGLSPDGTVALGTLALSEDARWLAFATA
                     ASGSDWNDIHVREVATGADRPDLLRWVKFSEIAWTHDHAGFFYSRYPAPADDNPLLAV
                     NRFHRLYYHRLGTSQEEDRLVYERPDQPDWGIGARITEDG"
     gene            1004..2572
                     /locus_tag="AC16JC_06565"
     CDS             1004..2572
                     /locus_tag="AC16JC_06565"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR5164394.1"
                     /translation="MKREILISGTPRETRVAILEDDRLVELLVDRPDTRRTVGDIHLG
                     RVEAVLPGIQAAFVDIGAEKSAFLHASDLVEAEDEEDPAEASDDDEDVTEGNGSGGGR
                     GRSRRRVPNVSEELKRGEAKLVQVIKEPISTKGPRVTAQVSLAGRFLVYMPFASRVGV
                     SRKIEGREQRTKLREMVSKLVPKDEGGWIIRTVADDLTEDKCRREIDHLLGVWKKVQR
                     KSTSVRAPALLQRETTLTRGIVRDLFSDKVDSLLVDSRALYGEIEQYLKQIDPELLER
                     VRLYREETPLFDKYDIEAEIRGLFQQRVDLPTGGYLIIQPTEALTSIDVNTGRYTGKK
                     DPESTILRTNVEAAREVARQLRLRDIGGIVVVDFIDMETRGNRDKVLQELRTHLGRDR
                     ARTKAFAVSELGLVEMTRQRVRPSLLQSMTAACPTCSGTGRVFRPEVVVRRMERSLKR
                     AGADHKERQLAVRLHPEVALYLVEQEPNFLRQLEKQTGLELEVRDDPMMRLDEFRMMA
                     KPAGRDVTEQYAVA"
     gene            2653..2970
                     /gene="rplU"
                     /locus_tag="AC16JC_06570"
     CDS             2653..2970
                     /gene="rplU"
                     /locus_tag="AC16JC_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HIR5164395.1"
                     /translation="MSYAIFVSGGQQFRAEPGRTIQVPLLEAEPGATVTFDRVLLTSD
                     GKTVQAGQPVVRGAKVTAEVVRHGKGPKIKIYRFARRAGYRRHAGHRQPFTEVKIADV
                     TVG"
     gene            2978..3250
                     /gene="rpmA"
                     /locus_tag="AC16JC_06575"
     CDS             2978..3250
                     /gene="rpmA"
                     /locus_tag="AC16JC_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002738127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR5164396.1"
                     /translation="MAHKKGVGSSRNGRDSQPKYLGVKRYGGEFVTAGSIIVMQRGTK
                     FHPGTNVRRASNDSLFSVVDGHVKFEHKDKKRMQVSVYPRQGTVGG"
     gene            complement(3282..3656)
                     /locus_tag="AC16JC_06580"
     CDS             complement(3282..3656)
                     /locus_tag="AC16JC_06580"
                     /inference="COORDINATES: protein motif:HMM:NF017451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5164397.1"
                     /translation="MLPYERLTAWRVCYTLVLETYRATEGFPRHELYGITSQTRRAAF
                     SAAANIAEGSAKHGPKEFRRYLDISIGSLSELAFALRLARDLEILPKDDWLRLDELRR
                     RTGFLVWRLYRAMSKKGRQANA"
     gene            complement(3772..4980)
                     /locus_tag="AC16JC_06585"
     CDS             complement(3772..4980)
                     /locus_tag="AC16JC_06585"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5164398.1"
                     /translation="MIPFLLAQTIAITGGTVYTVSGPKIEHATVLIRDGRITAVGTSV
                     TVPAEATRIDATGKWITPGLIDGAGQMGLVEIGAVPGTREGRIQGDTIAASFNVAEGI
                     NPASQVIPVTRVEGITTTLAAPGGHLVSGQAVLIDLDGATIEQMLVKSPVGIVADLSE
                     NGKDEGGGSRASMVQRLRATFRDALEYERRRLDYRRAQIEPLSASEADLEGLLPVLHG
                     ELPLIAIANRRSDIQSALRLAREFKLRLILAGAQEGWEIGTEIAAAGVPVLVQPLDNI
                     PSYDALGVRYDNAALLARAGVKVVLLETDTHNSRNIRQEAGNAVANGLPWDQALRAIT
                     LSPAEVFGVADRYGSLEAGKVANVVVWSGDPFEFSTGVEHVYIRGREIPLTSRQRELF
                     ERYRRLPPGY"
     gene            complement(4977..>5406)
                     /locus_tag="AC16JC_06590"
     CDS             complement(4977..>5406)
                     /locus_tag="AC16JC_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004274152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5164399.1"
                     /translation="DWWGFKEEAMDGIHENAALLQAAGARVVIHSDDPGGIQRLNQEA
                     GKAMYAGNRGGVPVTRDQALRWITANPAWVLGIDSIVGTLEPGKMADVVVWSADPFSV
                     YARALQVYNDGWLVYDRNDPAHQPRTDFELGQVPAPGGVR"
BASE COUNT          888 a         1768 c         1906 g          844 t
ORIGIN      
        1 ccgtcctcgg tgatgcgagc gccgatgccc cagtcgggtt gatccgggcg ctcgtacacg
       61 aggcggtcct cttcctgcga cgtgccgagg cgatgatagt agaggcggtg aaaccggttc
      121 accgcgagca gcggattgtc gtcggcgggg gcggggtagc ggctgtagaa gaaccccgcg
      181 tgatcgtgcg tccaggcgat ctccgagaac ttcacccacc gcagcagatc gggacggtcc
      241 gcgccggtcg ccacctcccg gacgtgaatg tcgttccaat ccgagccgct ggcggcggtc
      301 gcaaaggcca gccagcgggc gtcctccgag agggcgagcg tcccgagcgc caccgtgccg
      361 tccggcgaca gaccgttggg gtcgagcagc acggacggct cggcgtcacg ggacggctgg
      421 cggtacagca cggcctgatt ctggaggccg tcgttcttga agaagaagta ctgcccgcgc
      481 ttcttgaacg gcgtaccgta gcggggataa tcccagatcg cggtgagccg gcggcggatg
      541 gcctcgcgct cggggacacc tgcgagaaat tcgagcgtga gggcgttttg cgcgtcgatc
      601 caggcccggg tgtcgggtgc atctacgtcc tcgagccagc ggtagggatc cgccacccgg
      661 gtgccatggt agtcgtccac ctggtcaccc cgctgcgccg cggggtagcg caatcgcggg
      721 acgacgcctg gagcggctgc cggatcgggt agcgtatcgg ctgggcgcgg catgggcggc
      781 cgtaaattag gccgcattga cgtaagtcac aaggcgactt acatttacca catgtaccgg
      841 acgtgggtct cggttgcccg tttgtgcgtg cgccccgggc gcgcggcgcg agcggagtcc
      901 ggtccgccgg cccgtcgcag aacgctcact ggcccaagcg attgttggaa catcatgagg
      961 atcccgcgtg gctgttggcc ccgcgggatc gagggtgcgt gctttgaagc gcgagatact
     1021 gatcagcggg accccgcggg agacccgcgt ggcgattctg gaggacgacc gccttgttga
     1081 actgctggtc gatcgtcccg acacccgccg cacggtcggc gacatccatt tgggccgtgt
     1141 cgaagcggta ctgcccggta ttcaggcggc gttcgtcgac ataggcgcgg agaaaagcgc
     1201 cttcctgcac gcctccgacc tcgtcgaagc cgaggacgag gaggatcccg cagaagcgtc
     1261 ggatgacgac gaggacgtca ccgagggcaa cggctccggg ggagggcgcg gccgctcgcg
     1321 gcggcgcgtg cccaacgtct ccgaggagct gaagcggggc gaggcgaagc tggtgcaggt
     1381 catcaaggag cccatcagca ccaagggccc ccgcgtcacc gcgcaggttt cgctcgccgg
     1441 ccggttcctc gtctacatgc cgtttgcctc ccgcgtcggt gtttcccgca agatcgaggg
     1501 ccgcgagcaa cgcaccaagt tgcgtgagat ggtgtcgaag ctcgtcccga aggacgaggg
     1561 cggatggatc atccgcaccg tcgccgacga tctcaccgag gacaagtgcc ggcgcgagat
     1621 cgaccacctg ctgggcgtct ggaagaaggt ccagcggaag tccacgtcgg tgcgggcgcc
     1681 cgcgctgctg cagcgggaga cgaccctcac ccgggggatc gtccgtgatc tcttcagcga
     1741 caaggtggat tcgctgctgg tggattcgcg tgccctgtac ggcgagatcg agcagtacct
     1801 gaagcaaatc gatccggagc tgctggagcg cgtccggctg taccgcgagg aaacgccgct
     1861 gttcgacaag tacgatatcg aagccgagat ccgcgggctg tttcagcagc gggtggacct
     1921 gcccaccggc ggatatctca tcatccagcc caccgaagcg ctcacctcga tcgacgtgaa
     1981 cacggggcgc tacaccggga aaaaggaccc ggagagcacg atcctgcgga ccaacgtgga
     2041 agcggcccgc gaggtggcgc ggcagctgcg gctgcgggac atcggcggca tcgtggtggt
     2101 ggacttcatc gacatggaga cccgcggcaa ccgcgacaag gtgctccagg agctgcgcac
     2161 ccacctggga cgcgaccggg cccgcaccaa ggccttcgcg gtgagcgagc tgggcctggt
     2221 ggaaatgacg cgccagcggg tgcggccgtc gctgctgcag tcgatgacgg ccgcctgtcc
     2281 gacctgctcg ggtaccgggc gggtcttccg gccggaggtc gtggtgcgcc gcatggagcg
     2341 ctccctgaag cgggccggtg ccgaccacaa ggagcgccag ctcgccgtcc ggctgcatcc
     2401 ggaagtggcg ctctacctcg tcgaacagga gcccaacttc ctgcggcagc tggaaaagca
     2461 gaccgggctc gagctcgagg tgcgggacga cccgatgatg cggctggacg agttccggat
     2521 gatggcgaag ccggcggggc gcgacgtcac ggagcagtat gcggtggcct gagaagggac
     2581 ctgggacggg ggacgaggga cgggtcggcg tcacttgacg gaactgcatg tccccgttag
     2641 agttcgcgcc ctatgagcta cgccattttc gtgtcgggcg ggcagcagtt ccgcgccgaa
     2701 cccggcagga cgattcaggt gccgctgctc gaggcggagc cgggcgccac cgtcaccttc
     2761 gaccgggtgc tgctgacgag cgacggcaag acagtgcagg ccggtcagcc cgtcgtgcgg
     2821 ggcgcgaagg tcacggcgga ggtcgttcgg cacggcaagg gcccgaagat caagatctac
     2881 aggtttgcgc ggcgcgcggg gtaccggcgg catgcgggac accgccagcc gttcaccgaa
     2941 gtgaagatcg ccgacgtgac ggtcggctga ggacaccatg gcgcacaaga agggcgtagg
     3001 ctcctcccgc aacggtcgcg attcgcagcc caagtatctg ggtgtgaagc gctacggcgg
     3061 cgagtttgtg accgcgggga gcatcatcgt gatgcagcgc ggcaccaagt tccatcccgg
     3121 gacgaacgtg cggcgcgcgt ccaacgattc gctcttcagc gtggtcgacg gccacgtgaa
     3181 gttcgagcac aaggataaga agcgcatgca ggtgagtgtg tatccgcggc aggggacggt
     3241 aggaggctga ggagacggga ggagtgacga gagggggggc gctaggcgtt tgcctggcgc
     3301 cccttcttgc tcatcgcccg atagagacgc cataccagga agccagtacg gcgccgcagc
     3361 tcatcgaggc gcagccaatc atccttcggc aggatttcga ggtcgcgggc cagtcgcaac
     3421 gcgaacgcga gctcggacag cgacccgatg ctgatgtcga gatatcgtcg aaactctttc
     3481 gggccatgct tcgccgatcc ttcggcgatg tttgccgcgg cggaaaaggc cgcacgtcgc
     3541 gtctgagagg tgatcccata gagctcatgt cgcgggaagc cttctgtcgc ccggtacgtc
     3601 tccagcacca gggtgtagca gactcgccag gccgtgagcc gttcgtaggg aagcacgccc
     3661 caatctcaca ctggttgtag ccatggaggg taccaatcga gcgctaccgc gagcattcca
     3721 gcgcccggcc taccgccccc cctaccgtcc cctgccgtcc cgtaccgccc cctagtatcc
     3781 aggtggcagt ctgcggtacc gctcgaacag ctcccgctgg cggctggtga gcggtatctc
     3841 ccgcccgcgg atgtagacgt gctccacgcc ggtcgagaac tcgaacgggt cccccgacca
     3901 cacgacgacg ttcgccacct tgccggcctc gagcgagccg tagcggtccg cgacgccgaa
     3961 gacctcggcc ggcgagaggg tgatggcccg gagcgcctga tcccacggca ggccgttggc
     4021 gaccgcgttg ccggcctcct gacggatatt gcgggagttg tgcgtgtcgg tctcgagcag
     4081 cacgaccttg acgccggccc gggccaggag cgcggcgttg tcgtaccgta cgcccagcgc
     4141 atcataggac ggaatgttgt ccagcggctg caccaggacc ggtacgccgg cggcggcgat
     4201 ctccgtcccg atctcccacc cttcctgcgc gccggcgagg atcaggcgga gcttgaactc
     4261 ccgcgcgagg cgcagagcgc tctggatgtc gctgcggcgg ttggcgatcg cgatgagcgg
     4321 cagctccccg tggagcacgg gcagcagtcc ctcgaggtcg gcttccgagg cggagagcgg
     4381 ctcgatctgg gcgcgacggt agtccaaacg gcgtcgctcg tactccagcg catcgcggaa
     4441 ggtggcgcgc agccgctgca ccatggacgc ccgcgagccg cctccctcgt ccttgccgtt
     4501 ctcggagagg tcggccacga tccccaccgg cgacttcacc agcatctgct cgatggtcgc
     4561 gccgtccagg tcgatcagga cggcctggcc gctcacgagg tggccgccgg gcgcggccag
     4621 cgtggtcgtg atcccctcca cgcgcgtgac ggggatgacc tgcgacgccg gattgatgcc
     4681 ctcggccacg ttgaacgagg cggcgatcgt gtcgccctgg atgcgcccct cacgcgtccc
     4741 gggcaccgcg ccgatctcca ccagccccat ctgcccggcg ccgtcgatga gcccgggtgt
     4801 gatccacttg ccggtggcgt cgatgcgggt ggcctcggcc ggtacggtca cgctcgtgcc
     4861 gaccgcggtg atccgcccgt cgcggatcag gaccgtggcg tgctcgatct tcggccccga
     4921 caccgtgtac acggtgccgc ccgtgatggc gatggtctgg gccagcagga acgggatcat
     4981 cggacacctc ccggcgccgg cacttggccg agttcgaaat cggtgcgcgg ctggtgcgcg
     5041 gggtcgttgc ggtcgtagac cagccagccg tcgttgtaga cctgcagcgc ccgggcgtac
     5101 accgagaagg ggtcggccga ccacacgacg acgtcggcca tcttccccgg ctcgagcgta
     5161 ccgacgatcg aatcgatccc cagcacccag gccgggttcg ccgtgatcca gcgcagcgcc
     5221 tggtcgcgcg tgacggggac gccgccacgg ttgccggcgt acatcgcctt gccggcctcc
     5281 tggttcagcc gctggatgcc gcccgggtcg tccgaatgga tcaccacccg cgcccccgcg
     5341 gcctggagca gcgccgcgtt ctcgtgaatg ccgtccatcg cctcttcctt gaacccccac
     5401 cagtcg
//
LOCUS       DBNGZW010000275         4948 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1115713, whole genome shotgun sequence.
ACCESSION   DBNGZW010000275 DBNGZW010000000
VERSION     DBNGZW010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4948)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4948)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4948
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1115713"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..885)
                     /locus_tag="AC16JC_06595"
     CDS             complement(<1..885)
                     /locus_tag="AC16JC_06595"
                     /inference="COORDINATES: protein motif:HMM:NF052757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5164400.1"
                     /translation="MGETLSRFCLGRPVYVALLLALPTVSGCVSHGSAGDSHAAVGDS
                     TGAVDPGAPRGATADVLIVHDSDLLSMGLASAAVERRAFHHEIRTTASVEFDLERVVD
                     QTVRVGGWLERVNAYPQDRVRAGDTLALVYSPDYLVAQSNLLQARRQADAARAAGDSA
                     DLRTAAALVESSRQRLRLLGASERAVTRLLETGVTEPALAVVAPLSGTVAESDATRGA
                     AVSPGDRLFRIADLSTVWLRVDLYERDLGYVHAGDRVTVSVNAYPGVDFHGRVTRIGG
                     VLDTATRTVRARAAVANPD"
     gene            complement(891..2138)
                     /locus_tag="AC16JC_06600"
     CDS             complement(891..2138)
                     /locus_tag="AC16JC_06600"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR5164401.1"
                     /translation="MALLRTKCLALLFLPLAAQPPAHAQELREPLTLERAQDLGADSN
                     PRVLRAAAAVLTTRGTARTLGALSDPEVWYDFEGEEGVPFGSQRFGLTQVLEFPGKRG
                     LQGRIGSDAVDMEAERLAMVRNQVRAAIAEPFYQVARNDAALRSFATLDTLFVRLLEI
                     TRTRYAAGRATYDDVLRVQIERTRVAGAALRARGDRAEQVIALDVMLGRPVDHAVDVS
                     GDVLRFDSLPGPRAEFVAGRLAQSHSLRIARTLTIQRARALALARKANLPDFTLDASL
                     QHDRAQVHATYFSGGIALTLPLWRTRQAGLMQEARGESEEAAIGYRETEARVAGEAAR
                     AYEAAAALADEVQLFENRLLPQIEAGLESAIQAYGLGQTEILDVIDLVRTLRETRLEH
                     LGLLADYLASRARLAYAGDLEQE"
     gene            complement(2257..3597)
                     /locus_tag="AC16JC_06605"
     CDS             complement(2257..3597)
                     /locus_tag="AC16JC_06605"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR5164402.1"
                     /translation="MNTAAAEADLARRDPDVPGLATVFDPDAFAAAVRRAAPHADVRR
                     GRILYLRYKPRALCRLSYRLDVGGEPVELDVRACRREDVGPLLSEADAEAVVPGPLGS
                     GRLLLEDHAVVITVFPNDLKLVTLRFLARADERRRVLQESLPDRPDLWDGELRRLGYR
                     PERRFVAELRADGNGKRTVLKAYTRKAYQRGKHNVGAFASGGPLRLARLLGFSDERRL
                     LAFEWLPGNTLLEHLAAPALDGEVVAGAGAALATLHDLQPYGLTAWTRQDEVAAVAGV
                     AAEIGFVSPPLARRADLLAQRIETVLASAPPLQLPMHGDFSAAQVLVAPHAGGRVAII
                     DLDWSCRGDPADDLGNLIAQAERQVLDGAHSPSWRESFITALLEGYRGATARPLPDRV
                     GLYTALSLFRRARHPFRTRESDWPLRTDVLLERAEAIVQESGRGGHREACGLGQ"
     gene            complement(3594..4574)
                     /locus_tag="AC16JC_06610"
     CDS             complement(3594..4574)
                     /locus_tag="AC16JC_06610"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR5164403.1"
                     /translation="MTANPTEIGQRQPVGSQLPDDPALPALAALREKGLAAIPTLGMD
                     GRPARLSMCGYSPGDRATVEARVWGPGGGHFAIKAYTSDPAPEAELYLAFAGAGLTAG
                     SGLRVPPLLAWDGNLKVLVMGWLEGRPLNEVIKDGQPERAGELASQWPRLAASLPFRL
                     GPPFGAARVLKEAGPFAAMLETGDAALGSTGRALIESLARAQPPEHTPRLVHGTLYVR
                     QFLDRGGDRGPGIIDWQRFGVGPLELDAGTFLATLWRRALLRPRYAPEASRVEAAFRA
                     GTAGLLDERSLAWHQAAALLRLAGRMLRRRPAAQAISLLARAAELAETSR"
     gene            complement(4571..>4948)
                     /locus_tag="AC16JC_06615"
     CDS             complement(4571..>4948)
                     /locus_tag="AC16JC_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164404.1"
                     /translation="GDMSPGQFLWTGERLALLDIDMFGYADPAYDVGHFQAQVERRCI
                     VDLTARPLANTWIACFEEAYRSAMPRVSPRNIAFYRGLTLVRKMYTVCRREPGRWQGP
                     VSLLAWRAQAALEEVVAAAERAR"
BASE COUNT          736 a         1766 c         1779 g          667 t
ORIGIN      
        1 atcggggttg gcgacggcgg cgcgggcccg caccgtccgg gttgcggtat cgagcacgcc
       61 gccgatccgg gtcacgcgcc cgtggaaatc gactcccggg taggcgttca ccgaaacggt
      121 gacgcggtcg cccgcgtgga cgtaaccgag atcccgctcg tacaggtcga cccgcagcca
      181 gactgtcgag aggtcggcga tgcggaatag gcggtcgccc gggctgaccg ccgccccgcg
      241 cgtcgcgtcg gattccgcga cggtgccgga cagcggggcg acgaccgcga gcgcgggttc
      301 ggtcacgccg gtctcgagca gccgggtcac cgctcgctcg ctcgcgccga gcaggcgcag
      361 ccgctgccgg ctcgactcga ccagcgccgc ggcggtgcgc aggtcggcgg agtcaccggc
      421 tgcccgggcg gcatcggcct ggcggcgggc ttgcagcaga ttggattggg cgacgaggta
      481 gtcggggctg tagacgagcg ccagcgtgtc gccggcgcgt acccggtcct gtggataggc
      541 gttcacgcgc tccagccatc ccccgacgcg gacggtctgg tcgaccactc gctcgagatc
      601 gaattcgacc gacgccgtcg tccggatctc gtggtggaac gcccgtcgct cgaccgcggc
      661 cgaggcgagc cccatggaga ggagatccga gtcgtggacg atgaggacat cggcggtcgc
      721 gccgcgcggt gcgccgggat cgaccgcgcc ggtcgaatcg cccaccgctg cgtgggagtc
      781 gccggcgctg ccgtgcgaca cgcatccgct gaccgtcggc agggccagca gcagcgccac
      841 gtaaaccggg cggccgaggc agaaccgcga cagagtctcg cccacggcgc ctactcctgc
      901 tccaggtcgc ccgcgtacgc gaggcgggca cggctggcga ggtagtcggc cagcaggccc
      961 agatgctcga ggcgggtttc ccgcagggta cgcaccagat cgatgacgtc cagaatctcg
     1021 gtctgcccga gaccgtaggc ctggatcgcg gattcgaggc ccgcttcgat ctggggcagg
     1081 agccggttct cgaacagctg gacttcgtca gcgagcgccg cagccgcctc gtaggcgcgc
     1141 gccgcctcgc ccgccacgcg ggcctccgtt tcccggtagc cgatggcggc ttcttcggac
     1201 tcgccgcggg cctcctgcat gaggcccgcc tggcgcgttc gccacaaggg aagggtcagc
     1261 gcaatgccgc cggagaagta cgtcgcatgg acctgggccc gatcgtgctg cagcgaggca
     1321 tcgagggtga agtcgggaag gttcgccttc cgggcgagcg ccagcgcccg cgcgcgctgg
     1381 atcgtgagcg tccgcgcgat gcgcagggaa tggctctggg cgaggcgccc ggccacgaac
     1441 tcggcgcggg gtccgggcag ggagtcgaac cgcagcacgt ctccggacac gtccaccgca
     1501 tggtccaccg gccgccccag catcacgtcc agggcgatca cctgctcggc ccgatcgccc
     1561 cgcgcgcgga gcgccgcccc ggcgacgcgg gtccgctcga tctgtacgcg gagcacgtcg
     1621 tcataggtcg cgcgacccgc ggcgtagcgg gtgcgcgtga tctcgagcag ccggacgaac
     1681 agggtgtcga gcgtggcgaa cgaacggagc gcggcgtcgt tgcgcgctac ctggtagaag
     1741 ggttcggcga tcgcggcacg gacctgattt cggaccatgg ccagacgctc cgcctccatg
     1801 tccaccgcgt cgctgccgat ccggccttgc aggccgcgct tgccggggaa ctccagcacc
     1861 tgcgtgagac caaaacgctg tgagccgaag ggcaccccct cttccccctc gaagtcgtac
     1921 cacacttccg ggtccgacag ggcgccgagc gttctcgcgg ttccgcgcgt cgtcagcacg
     1981 gcggcggcgg cgcgaagcac gcgggggttg ctgtcggctc ccaggtcctg ggcccgttcc
     2041 aaggtcaacg gttcccgcag ctcctgggcg tgcgcgggag gctgtgcggc gagcgggagg
     2101 aacaacaagg cgaggcactt cgtgcgtaac agcgccatca ctcatccctc gtgcgatatc
     2161 aggatccccg tacggcttgc aagataggcg ccagaaacga cctctgcttc gggcgtcagg
     2221 gggagaagcg agtggtctgg gcgaggagcg cgggcgctac tgtccgaggc cgcacgcctc
     2281 gcggtgcccg ccgcggcctg actcctgcac gatcgcctcg gctcgttcga gcagcacatc
     2341 ggtacgcaac ggccagtcgg actcgcgcgt gcggaacggg tggcgcgccc gccggaacag
     2401 gctgagcgcc gtatacaagc ccacgcggtc gggaaggggg cgcgcggtcg caccgcgata
     2461 gccctccagc agcgccgtga tgaaggactc gcgccagctc ggggagtgcg cgccgtccag
     2521 cacctggcgc tcggcttgcg cgatgaggtt gcccaggtcg tcggccggat ctccgcgaca
     2581 ggaccagtcg aggtcgatga tcgcgacccg cccgccggcg tgaggcgcga ccagcacctg
     2641 ggcggccgag aagtcgccgt gcatcggcag ctgcaggggc ggtgcgctcg cgagaacggt
     2701 ctcgattcgc tgcgccagca agtcggcccg gcgggccagc ggcggcgata cgaaaccgat
     2761 ctcggcggcc acaccggcca ccgcggccac ctcgtcctgg cgcgtccatg ccgtgagtcc
     2821 gtagggctgg aggtcgtgca acgtcgccag cgccgcgccg gccccagcca ccacttcgcc
     2881 gtcgagtgcg ggcgcggcga ggtgctccag cagcgtgttg cccgggagcc actcgaaggc
     2941 gagcaaccgg cgctcgtccg agaacccgag caagcgggcc agccgcagcg gcccacctga
     3001 agcgaacgcc ccgacgttgt gcttgccccg ttggtacgcc ttccgagtgt acgccttcag
     3061 cacggtccgc ttgccgttgc cgtccgcgcg cagctcggcc acgaagcgcc gttcgggccg
     3121 gtagccgaga cggcgcaact cgccatccca caggtcgggc cgatcgggca aggattcctg
     3181 cagcacgcga cgacgttcat ccgcccgcgc caggaaccgg agcgtgacga gcttgaggtc
     3241 gttggggaac accgtgatca ccacggcgtg gtcttcgagc agaaggcggc cgctgccgag
     3301 ggggcccggg acgacggctt ccgcgtcggc ctcggacagg aggggaccga catcctcccg
     3361 ccggcaggcg cgcacgtcga gctcgaccgg ttcgccgccc acgtccagcc gataactgag
     3421 ccggcacagg gcgcggggct tgtaccggag atagaggatc cggccgcggc gcacgtcggc
     3481 gtgcggggcg gcacggcgca ctgccgcggc gaacgcgtcg ggatcgaaaa ccgtggccag
     3541 gccgggtacg tcgggatcgc ggcgggccag gtcggcctca gccgcggcgg tgttcatcgg
     3601 gaggtctcgg ccagctccgc cgccctcgcg agtagcgaga tggcctgcgc agcaggccgc
     3661 cgtcgcaaca tgcgccccgc gaggcgcagc aacgctgccg cctggtgcca cgccagggag
     3721 cgctcgtcca gcagccccgc ggtgcccgct cggaaggcgg cttccactcg tgaggcctcg
     3781 ggcgcgtaac gcgggcgcag cagagcgcgg cgccacagcg tggcgaggaa ggtccccgca
     3841 tcgagctcca gcggtcccac gccgaaacgc tgccaatcga tgattccagg acctcggtcg
     3901 ccgccccggt cgaggaattg acggacgtac agcgtgccgt gcacgagccg aggagtgtgc
     3961 tccggcggtt gtgcccgcgc gagcgactcg atcagcgctc ttcccgtcga tcccagcgcg
     4021 gcgtcgccgg tctccagcat ggcggcgaac ggccccgcct ccttcaggac gcgggccgcc
     4081 ccgaacgggg gcccgagccg gaacggcagc gacgcggcga gccgaggcca ctgggatgcc
     4141 aactcgcccg cgcgctccgg ctgtccgtct ttgatgacct cgttgagcgg ccgcccctcc
     4201 agccacccca tcaccaggac cttgaggttt ccgtcccagg cgagcagcgg cgggacacgc
     4261 agtcccgagc ccgcggtgag gccggcgccg gcgaaggcca ggtacaactc cgcctccggc
     4321 gccggatcgc tcgtgtacgc cttgatggca aagtgccctc cacccggtcc ccacacccgg
     4381 gcctcgactg tggcccggtc ccccggcgag tagccgcaca ttgacagcct tgccgggcgg
     4441 ccgtccatcc cgagcgtggg gatggccgcc agccccttct cgcggagtgc cgcgagggcc
     4501 ggcagggccg ggtcatccgg cagttgcgac ccgacgggtt ggcgttgtcc gatttccgtc
     4561 gggttcgccg tcatcgtgcg cgctccgccg cggcgacaac ctcttccagc gccgcttggg
     4621 cgcgccaggc gagcaacgac accggtccct gccaacgacc aggctcgcgt cggcacaccg
     4681 tgtacatctt gcgtaccagc gtgagtccgc ggtagaacgc gatgttgcgc ggcgacacgc
     4741 gcggcatcgc cgagcggtac gcctcctcga agcaggcgat ccacgtgttc gccagtggtc
     4801 gcgccgtcaa gtccacgatg cagcggcgct ccacttgcgc ctggaagtgg ccgacgtcgt
     4861 aagccggatc ggcgtagccg aacatgtcga tatcgagcag cgccagccgc tcgccggtcc
     4921 acaggaactg cccgggcgac atgtcccc
//
LOCUS       DBNGZW010000276         8418 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_228988, whole genome shotgun sequence.
ACCESSION   DBNGZW010000276 DBNGZW010000000
VERSION     DBNGZW010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8418)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8418)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8418
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_228988"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="AC16JC_06620"
     CDS             complement(<1..1012)
                     /locus_tag="AC16JC_06620"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR5164405.1"
                     /translation="MTTRVVRTCWLGLLAVAAGCVETDRALQQPPSLDAQLRQSLQQW
                     GTVPINPPPAQDTALVSLGQALFFDKELSGNRDIACATCHSPGTAMGDGQSLAVGTGG
                     SGVGPTRVLGSGRSFVPRNAPTLLNATIAVPYLFWDGRVVGFAGGGQFETPAGAALPP
                     GLPNVLAAQAMFPVANRIEMRGAPGDTDVFGRPNELAQYGDSQWVEIWRAVMLRLMAI
                     PAYKVRFAAAFPGIPSAQLGFQHAATAIAAFETQALTRLDTPFDRYLRRDDHAMTAQE
                     KRGAVLFFGKATCSSCHNGPQLGAGSFVDVGVPQIGPGVGAEPPLDLGRGALPGNEFY
                     RFA"
     gene            complement(1026..2555)
                     /locus_tag="AC16JC_06625"
     CDS             complement(1026..2555)
                     /locus_tag="AC16JC_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164406.1"
                     /translation="MIPFGRCIVARRPAFLLLTLFLAPGTKAPAQIIPIRSVPLAQGD
                     QFLIFPTANLGMGGVSIALPDSLLDPFRNPAMGARVGAGRLFAAPTVYGISRETGGGR
                     TLPLALLGRAGPWFGGLSGAIQQVEYSGPMTSIAVPIGIDRVQAAILQSPVPIGDRSH
                     GNSYLQASLGRVLTGSGLSVGGGLFWSRLHAVSGVDLLYPGSQSLAQSGHQLDLRLGM
                     LKEWPGSRSLEALVLHDRYSMTDDVSYLDLFWDAGTQQFVQVPRVEHELDRLTTTGVH
                     LSYQRPLAASGWRIGWIATVNVDRQPRIAPDEIVTLPRDQGHSTAYNLGIGLSKIEGP
                     STFGLDAVYEPIWSTTWAVTSTPIMTALGDTIPAGGRTGDNRFRFSNVVLRLGVSQDM
                     PLAAVKRGVGLQLGLAVRSMHYRLVERDYLAGTSQPFRDSWVEWSPTWGLSLRFPQLE
                     LHYRGRVTNGTGRPSTFNIGFANPLAASPTSMLLPPGGTLGLAGVSMVTHQISLSLPL
                     R"
     gene            complement(2639..3340)
                     /locus_tag="AC16JC_06630"
     CDS             complement(2639..3340)
                     /locus_tag="AC16JC_06630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylesterase"
                     /protein_id="HIR5164407.1"
                     /translation="MRRIIVSAALAGLVACSGRPRDAVPAAADRTADAVPPVDERPVV
                     LCFGTSLTAGLGLAPDDAYPAQLQRRVDSAGLGFRVVNAGVSGETSAAGLRRIDWLLE
                     QPQQVAVLLLELGANNALRGQDLGAAKRDLQAIIDRTRARFRGVRIVVAGMAAPPNLG
                     TRYTREFRDMFVDLARANRAALIPFLLEGVAGRPELNQADGIHPTAEGERLVTDNVWK
                     VLEPVLRSVTRETGR"
     gene            3342..4037
                     /locus_tag="AC16JC_06635"
     CDS             3342..4037
                     /locus_tag="AC16JC_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009284316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5164408.1"
                     /translation="MIEAQDLEQTYQSGGRPLTVLRQINLAIAPGEFVAIMGPSGSGK
                     TTLLGLLAGLDRPTRGTIRLDGEDLGALSEDARARIRVEKVGFVFQTFQLMPTLTALE
                     NVLVPIELRGRAAGNGRAAEVRARQLLERVGLGDRVDHYPAQLSGGEQQRVGLARAFA
                     AEPRVLLADEPTGSLDAETGRAVIDLLVQLNRDAGTTLVLVTHDPALAARARRVIRIS
                     AGAIVSDTRTEPA"
     gene            4034..6577
                     /locus_tag="AC16JC_06640"
     CDS             4034..6577
                     /locus_tag="AC16JC_06640"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5164409.1"
                     /translation="MRAPFALVMAWRESRGSRRRLALYLGAVSLGVAALVAINAFRAD
                     VTASVHAQARTLLGADLALRSRSPFPDSVEAVFDSLRASGVPVARVTSFASMALAVRS
                     GRTRLVDVRAVAGGFPFYGTIATEPPGLWTTFRGRRAVLVDPAVLVYLEATPGDTVQI
                     GDTRFVIAGVVSDAPGDVSLWSAIGPRVYLPAEYLGETHLLQFGSLAQYRAYFQLPDA
                     AVQRFLNHFNKLFQRHLIGSETVSEQEEDLTRALGRLARYLGLVGLIALLLGGLGVAS
                     AVNVFVRDKLDGAALLRCLGARQRTVLAVYLLQAAALGLIGAGVGVMLGVLVQAALPA
                     MLGEFLPLDVAVTLQWSTMLTGLGIGVGTACLFALLPLLQLKEVSPLRVLRRELDAGS
                     VPRPARWWRLLVAAMMVAGLVALSRWQAPRPLVGYAFAAAVLVTTAALWLTAKGLIWA
                     TRRWFPHRASYVIRQGIANLFRPQNQTVAVMLAIGFGVFLITTLYVVQRSLVAQFALD
                     TQPDRPNLVFFDVQPDEKDSVAGILAARGVRPLDLAPIVPARIARVNGRPIDSLSSDS
                     LHRRSPWVLRREYRNTYRDTLVGSERLTAGTWWTATRPHGGLPRVSLEEDIASSLGVG
                     LGDRITWNVQGVLVETRVASFRQVSWARFQPNFFVVFEPGVLERAPQTFVLLARTTDA
                     ALRAELQRDVTLAYPGVSSLDLTLVQRTLDSLITKVTLAIRFMALFSIGSGLVILIGA
                     LTASRLQRAREAVLLKTLGASTRQVRGIFLTEYSTWGSLAALTGVVLAGAGGWAIMTR
                     LFELPFRLPVLPLLGVWLAVCGLVVAVGLAHSRTVVRGTPLAMWRTLSE"
     gene            complement(6578..7453)
                     /locus_tag="AC16JC_06645"
     CDS             complement(6578..7453)
                     /locus_tag="AC16JC_06645"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR5164410.1"
                     /translation="MTLARLLTVWLGALPAMGSQGAAQVAWRPASPLQGSLVMLGVRV
                     PATDSVRVVRGELAGEPLHFELIDGWYRAIGGVPIGARTHVRARVVIERSSGALDTLT
                     RRVPVRARRTPSERLRTDPAFVQPPESLAPRLQAERELVRDLRRGAHTVPRLWSEAFV
                     QPRPGPVTSVFGMRRVFNGRLRSRHLGADFSGRSGDSVLATNRGVVAYVGDLYFNGTT
                     IFLDHGAGLLTAYLHLSRVLVAAGDTVSGGQLIGFVGASGRVTGPHLHWFASYGDVTV
                     DPTDLLRVDLSASLP"
     gene            7677..8225
                     /locus_tag="AC16JC_06650"
     CDS             7677..8225
                     /locus_tag="AC16JC_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5164411.1"
                     /translation="MPFAPATLREHLRTLTAHGFVQRQGNRRGKRGRPEVVYALTRRG
                     DALFPHGEAAVLRDLVVQLAEDGHLQLLERFFARRIAAQRPAALARVRGLDGAARFSE
                     VAQILAEDGFMAQVEGSPTGPTLRICHCPIRDLVDVTQAPCRYEQALIAELLGHPLER
                     IEYLPEGRVSCLYREAVAVPQP"
BASE COUNT         1206 a         2977 c         2933 g         1302 t
ORIGIN      
        1 acgcgaagcg atagaactca ttgcccggca gcgcgccgcg tcccaggtcg agcggcggct
       61 cggcccccac gccgggcccg atctgcggca ctccgacgtc cacgaagctc cccgccccga
      121 gctgcggacc gttgtgacaa ctggagcagg tcgctttgcc gaagaagagg accgcgcccc
      181 gtttctcttg cgcggtcatc gcgtggtcgt cgcgccgcag gtaccgatcg aacggcgtgt
      241 cgagtctggt gagggcctgg gtctcgaacg ccgcgatggc ggtcgccgcg tgctggaacc
      301 cgagctgagc gctggggatt ccggggaacg ccgcggcgaa gcggaccttg taggcgggaa
      361 tcgccatcag acgcagcatc accgctcgcc agatctccac ccactggctg tcgccgtact
      421 gggcgagctc gttcggtctc ccgaacacgt cggtgtcacc gggcgctccg cgcatctcga
      481 tgcggttggc gacggggaac atcgcctgcg ccgcgagcac gttcggtaac ccgggcggca
      541 gcgccgcccc ggctggcgtc tcgaactgcc ctccccctgc gaagcccacg actcggccat
      601 cccagaagag gtacggcacg gcgatcgtcg cgttcaggag cgtcggcgcg ttgcggggca
      661 cgaatgaccg gccggacccg agcacgcggg tgggcccgac gccggacccg ccggtaccga
      721 ccgcgagcga ctgcccgtcg cccatcgcgg tacccgggct gtggcacgtc gcgcaggcga
      781 tatcgcggtt gccgctcaat tccttgtcga agaacagcgc ctgcccgagc gacaccagcg
      841 ccgtatcctg ggcgggcggt gggttgatcg gcacagtgcc ccactgctgc agcgactgcc
      901 gtagctgggc gtcgagcgat ggcggttgct ggagggcgcg atcggtctcg acgcacccgg
      961 cggcgacggc caacaacccg agccagcagg ttctcaccac gcgtgtcgtc atggtcggcc
     1021 tcccgtcagc gcagcggcag ggacagcgag atttgatgcg tgaccatgga gacgccggcc
     1081 aggccgagcg tcccacctgg aggcagcagc atgctggttg ggctcgccgc gagcggattg
     1141 gcgaagccga tgttgaaggt gctcggccgg ccggtgccgt tcgtgacgcg gccacggtag
     1201 tgcagctcga gctgcgggaa ccggaggctc aggccccagg tgggcgacca ttcgacccag
     1261 ctgtcgcgga acggttggct ggtgccggcc aggtagtcgc gctcgaccag ccggtagtgc
     1321 atcgagcgca cggcgaggcc caactgcagc cccacgcctc gcttcacggc ggccagcggc
     1381 atatcctggc tcacccccaa gcgcagcacg acgttcgaga accggaagcg gttgtcgccg
     1441 gtccgtcccc cagccggaat cgtgtcgccc agcgcggtca tgatcggcgt actcgtcacc
     1501 gcccaggtcg tgctccagat cggctcgtag acggcgtcga ggccgaacgt gctgggaccc
     1561 tcgatcttgg acaaccctat tcccaggttg tacgcggtgg agtgtccctg gtcgcgcggg
     1621 agcgtcacga tttcgtcggg cgcgatgcgc ggctgccggt ccacgttgac ggtcgcgatc
     1681 cagccgatgc gccagcccga cgccgcgagc ggccgctgat aggagaggtg cacgccggtc
     1741 gtggtgaggc gatccagctc gtgctccact ctcgggacct ggacgaactg ctgcgtcccc
     1801 gcgtcccaga acaggtcgag gtagctgacg tcgtcggtca tcgagtagcg gtcgtgcaga
     1861 accagggctt ccagcgaccg gctgcccggc cattctttca gcattcccag ccggagatcg
     1921 agctggtgcc ccgattgggc gaggctctgg ctgcccgggt agagcaggtc cacgccgctc
     1981 accgcgtgca gccgggacca gaagaggccg cccccgacgg agaggccgct tcccgtcagc
     2041 actctgccga gggaagcctg caggtaggaa ttaccgtgcg agcggtcacc gatcgggacg
     2101 ggggactgga ggatcgccgc ctggacgcga tcgatcccga tgggaaccgc gatcgacgtc
     2161 atcggtccgc tgtactcgac ctgctggatc gccccggaca acccgccgaa ccacgggccg
     2221 gcacgcccca ggagggcgag cggcagggtg cgcccgcctc cggtttcgcg ggaaatcccg
     2281 tacacggtgg gagcggcgaa caggcgtccc gcccccaccc tcgcacccat ggccgggttc
     2341 cggaacgggt cgagcagcga gtccggcagc gcgatggaca cgccgcccat ccccaggttg
     2401 gccgtcggga agatcaggaa ctgatcgccc tgggcgagcg ggacgctccg gatcggaatg
     2461 atctgcgcgg gggctttggt gccgggtgcg aggaacaggg tcagcaacag gaacgcggga
     2521 cgccgggcta cgatgcaccg accgaaaggg atcatctgac accccggctg gaggaggttg
     2581 agagtgaata cccgggcggg gctcgtcgcg tcacacagcg gaacccacct cgcgaaggtc
     2641 acctgcctgt ttcacgcgta acgctccgca gcaccggctc cagaaccttc cacacgttgt
     2701 ccgtcacgag gcgctcaccc tccgcagtcg ggtggatgcc atcggcctgg ttgagctcgg
     2761 gccgacccgc cactccttcg agcagaaacg gaatcaacgc ggcccggttg gcccgggcca
     2821 gatcgacgaa catgtcgcgg aattcgcgtg tgtagcgggt cccgagattc ggtggcgcgg
     2881 ccatcccggc gacgacgatg cgcacgccgc ggaatcgcgc ccgggtccga tcgatgatcg
     2941 cttggagatc ccgtttcgct gcgccgaggt cctgtccgcg cagcgcattg ttggcgccca
     3001 gctcgagcag cagcacggcc acctgttgcg gttgctccag gagccaatcg atgcgtcgca
     3061 gcccggcagc cgaggtctcg ccgctcacgc cggcgttcac gacccggaat ccgagtcccg
     3121 ccgaatcgac tcggcgctgc agctgcgccg ggtaggcgtc gtcgggcgcg aggcccagtc
     3181 ctgccgtgag gctcgtcccg aaacagagca cgaccgggcg ttcgtcgacc ggcggcaccg
     3241 cgtcggccgt gcggtcggcc gcggccggga ccgcgtcgcg ggggcgcccg ctgcaggcga
     3301 cgagtccggc cagcgccgcg ctcactatta ttcgccgcat gatgatcgaa gcccaggacc
     3361 tcgagcagac gtaccagagc ggcggccggc cgctcaccgt gctgcgccaa attaacctcg
     3421 ccatcgcccc gggagagttc gtggcgatca tgggaccgtc gggcagcggc aagacgaccc
     3481 tgctcggcct gctcgccggt ctcgatcgtc ccacgcgggg gaccatccgt ctcgacggcg
     3541 aggatctggg cgcgctgtcc gaggacgccc gggcccggat acgagtcgag aaagtcggct
     3601 tcgtgttcca gaccttccag ctgatgccca cgctgaccgc gctggaaaac gtcctcgtcc
     3661 cgatcgagct gcgcggcagg gccgcgggca acggccgcgc ggcggaggtg cgcgcccgtc
     3721 agttgctcga gcgcgtgggg ctcggcgacc gggtggatca ctaccccgcg cagctgtcgg
     3781 gcggcgagca gcagcgcgtg ggcctcgccc gcgcgttcgc cgccgagccc cgcgtgctgc
     3841 tggccgacga gcccacgggt agcctcgacg ccgaaaccgg ccgcgcggtg atcgacctcc
     3901 tggtgcagct caaccgcgac gcgggaacga ccctcgtcct ggtcacgcac gatccggcgc
     3961 tggctgcccg ggcgcgccgc gtgatccgga tctccgccgg agcgatcgtt tccgacactc
     4021 gcacggagcc cgcgtgagag cgccgttcgc gctcgtcatg gcctggcgcg agagccgcgg
     4081 cagccggcgg cgcttggcgc tctacctcgg tgccgtgagt ctcggcgtcg ccgcgctggt
     4141 ggccatcaac gcgttccggg ccgacgtcac ggcgtcggtg cacgcgcagg cccgcacgtt
     4201 gctcggcgcc gacctggcgc tccgctcgcg gtccccgttc ccggactcgg tcgaagccgt
     4261 gttcgactcg ctgcgcgcgt ccggcgtgcc ggtcgcccgg gtgacgagct tcgcctccat
     4321 ggcgctcgcc gttcgcagtg ggcgcacgcg gctggtcgac gtccgcgccg tcgcgggagg
     4381 ctttcccttc tatgggacga tcgccaccga gcccccggga ctctggacga cgttccgcgg
     4441 ccggcgcgcg gtgctcgtgg accccgccgt gctcgtctac ctggaggcta cgccagggga
     4501 tacggtgcag atcggcgaca cgcgattcgt catcgccggc gtcgtcagcg atgcgcccgg
     4561 cgacgtctcg ctctggtccg cgatcggccc gcgcgtctac ctccccgccg agtacctggg
     4621 cgagacccac ctgctccagt tcggcagcct ggctcagtac cgcgcgtact tccagctgcc
     4681 ggacgccgcg gttcaacgat tcctcaacca cttcaacaag ctgttccaac gccacctgat
     4741 cggcagcgag accgtgtcgg agcaggagga ggatctgacc cgggcactcg gccggctggc
     4801 ccggtatctc ggcctggtcg ggctgatcgc gctgttgctc ggcgggctcg gcgtcgccag
     4861 tgccgtgaac gtcttcgtgc gggacaagct cgatggcgcc gcgctgttgc gctgtctcgg
     4921 cgcccggcag cgcaccgtgc tcgcggtcta tctgctccag gccgctgccc tcggcctgat
     4981 cggagcgggc gtgggcgtca tgctgggtgt gctggtgcag gccgcgctgc ctgccatgct
     5041 cggtgagttc ctgcccctcg acgtggcggt cacgctgcaa tggtccacga tgctcacggg
     5101 actcgggatc ggcgtcggca ccgcgtgcct gttcgcgctg ctgccgctcc tgcaattgaa
     5161 ggaggtgagc ccgctgcgcg tgctgcgccg tgagctcgac gccgggtccg tacctcggcc
     5221 cgcgcgatgg tggcgcctgc tcgtggccgc gatgatggtc gccgggctcg tggcgctgtc
     5281 gcgctggcaa gcgccgcgcc cgctcgtcgg ctacgcgttc gccgcggccg tgctcgtcac
     5341 caccgccgca ctctggctca cggcgaaggg cctcatctgg gcgacgcgac gctggttccc
     5401 ccatcgggcg agctacgtga tccgtcaggg catcgcgaat ctgttccggc cccagaacca
     5461 gacggtcgcc gtgatgctcg ccatcggctt cggcgtcttc ctcatcacga cgctatacgt
     5521 cgtgcagcgc agcctcgtgg cccagttcgc gctcgacacg caacccgatc ggccgaatct
     5581 ggtgttcttc gatgtgcagc cggacgagaa ggacagcgtc gccgggatcc tcgcggcgcg
     5641 cggcgtgcgg ccgctcgacc tggcgccgat cgtcccggcc cgcatcgcac gcgtgaacgg
     5701 ccgtcccatc gattcgctgt cgtcggattc cctccatcgt cgcagcccct gggtgttgcg
     5761 gcgggaatac cgcaacacct accgcgacac gctggtgggc tcggagcgcc tgacggcggg
     5821 gacctggtgg accgccaccc ggccgcacgg ggggctgccc cgcgtctccc tcgaggagga
     5881 catcgcgtcg tcgctgggag tcggcctcgg cgaccgcatc acctggaacg tgcagggcgt
     5941 gttggtggag acgcgcgtcg cgagcttccg ccaggtgagc tgggcgcggt ttcagccgaa
     6001 cttcttcgtc gtcttcgagc ccggtgtgct ggagcgcgcc ccgcagacct tcgtcctgct
     6061 cgcccggact accgacgcgg ccctgcgcgc cgagctgcag cgcgatgtca ccctcgccta
     6121 tcccggcgtc tcgtccctcg atctcaccct cgtgcagcgg acgctcgatt ccctcatcac
     6181 caaggtcacc ctcgccatcc ggttcatggc gttgttctcg atcggcagcg ggctcgtgat
     6241 cctcatcggt gcgctcacgg catcgcggct ccagcgcgcc cgcgaggcgg tactcctgaa
     6301 aacgctcggg gccagcaccc ggcaggtgcg cggcatattc ctcaccgagt actccacctg
     6361 gggctcgctc gcggcattga ccggcgtcgt cctggccggt gcgggcggct gggcgatcat
     6421 gacgcggctg ttcgagctgc cgttccggct cccggtgttg ccgctgctcg gggtgtggct
     6481 ggcggtctgt gggctggtcg tcgcggtcgg gctggcgcac agccgcaccg tcgtgcgcgg
     6541 gacgccgctc gcgatgtggc gtacgctgag cgagtgatca gggcagcgac gcgctcaggt
     6601 cgacccgtag gagatcggtg ggatcgaccg tgacgtcgcc atacgaggcg aaccagtgca
     6661 ggtgcggacc ggtcacccgc cccgaagcgc cgacgaagcc gatgagctgg ccccccgaga
     6721 ccgtgtcacc cgccgctacc agtacccggc tgaggtgcag gtaggcggtg agcagacccg
     6781 cgccgtggtc gaggaagatg gtcgtgccgt tgaagtacaa atcgccgacg tacgccacga
     6841 cgccgcggtt ggtggcgagc accgaatcgc cgctgcgacc ggaaaaatcc gcccccaggt
     6901 gacggctgcg cagccgaccg ttgaacaccc gccgcatccc gaagacgctg gtgaccgggc
     6961 cgggccgtgg ctgcacgaat gcttcgctcc acagacgggg cacggtgtgg gccccgcgcc
     7021 ggagatcacg gaccagctcc cgttccgcct gcaatcgggg ggcgagcgac tccggcggct
     7081 gcacgaaggc cggatcggtc cgtaaccgct cgctcggcgt ccgccgcgcc cggacgggca
     7141 cgcggcgggt cagcgtgtcg agggcgccac tggaccgctc gatcaccacg cgtgcccgga
     7201 cgtgcgtgcg agcgccgatg ggcacgccgc cgatcgcgcg gtaccacccg tcgatgagct
     7261 cgaaatggag cggttcgccg gcgagctcgc cccgcacgac acgcacgctg tccgtcgccg
     7321 gcacgcggac accgagcatg acgagtgaac cctggagggg actcgccggc cgccacgcca
     7381 cctgcgccgc gccctgactc cccatcgccg ggagtgcgcc gagccagacg gtgagcagcc
     7441 tcgccagtgt catgcgcgtt agtactccat cgctctctga aggtcccttc agtcggactt
     7501 tcgtattcac ttagacgtcc ccatctaccg tgtttgccat ggaatcggcc agcgcaaaca
     7561 cgagcagagc ttctaccgcg ccgctgttcg ggcccggtct gggggaaacc cagcgggcca
     7621 tccttcttct attgaagcgg ctcggaccgg cgacccaggc agaggtgggg cgcgaggtgc
     7681 cgtttgcacc tgcgaccctg cgcgagcacc tccggacgct gacggcgcac gggttcgtgc
     7741 agcgtcaggg gaaccggcgc ggcaagcgag gccgacccga agtggtgtac gccctcaccc
     7801 ggcgcgggga tgcactgttt ccgcacggcg aagcggccgt gctgcgcgat ctcgtcgtgc
     7861 agctcgccga ggacggacac ctccagctgc tcgagcggtt cttcgcccgg cggatcgcgg
     7921 cgcaacgccc tgccgcgctg gcgcgtgtgc gcgggctcga cggcgccgcc cgattctccg
     7981 aggtggccca gattctcgcc gaagacgggt tcatggccca ggtggagggc tcgcccaccg
     8041 gacccaccct ccggatctgc cactgtccga tccgcgacct ggtcgacgtc acccaggcgc
     8101 cgtgccgtta cgagcaggcg ttgatcgccg agctgctcgg acatccgctc gagcgtatcg
     8161 agtacctccc cgaagggcgg gtctcgtgcc tgtaccgcga ggcggtcgcc gttccccaac
     8221 cttagatcgg gagtttcggg gatggcggcc gtagcgtcgc cgaccgtgag caagcgtcgc
     8281 acgcgtgacg cagcctcggg cgggcgggtc gcagacccga tgcgaatcac gacgacgatg
     8341 acgagccgga cacaggtatg cggattggtg atcatggtgg cgctcctcgc gccgcagcct
     8401 gggcgcgccc aggagcgc
//
LOCUS       DBNGZW010000277         1706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1721332, whole genome shotgun sequence.
ACCESSION   DBNGZW010000277 DBNGZW010000000
VERSION     DBNGZW010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1706
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1721332"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..465
                     /locus_tag="AC16JC_06655"
     CDS             106..465
                     /locus_tag="AC16JC_06655"
                     /inference="COORDINATES: protein motif:HMM:NF015690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HIR5164412.1"
                     /translation="MASPLDPQARRRQLEQARALFNDGEYWLAHEALETVWRSIIDEG
                     ERRVWQGLIQAAAALLHRRRGNRHGTVVVGAAALEKLSGPQHPAVEFDTLRFRAVLAR
                     ALAGEGDPPRLDFRNDA"
     gene            458..1441
                     /gene="trxB"
                     /locus_tag="AC16JC_06660"
     CDS             458..1441
                     /gene="trxB"
                     /locus_tag="AC16JC_06660"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003321872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR5164413.1"
                     /translation="MPESGQQSYDVIIVGAGPAGLCAAMYAGRGMLKALTIERGAPGG
                     ELLNTDLVEDYIGFESIKGWDLAQKFTDHAKKFGAEIVTDTVESVRRAPDGWFDLTTA
                     RERHYRAPAVILTAGGTPVKLGVPGEREYAGKGVSYCAVCDGAFFKGEVLAVVGGGDA
                     AVEEADYLTRYASKVYIVHRRDRFRASRILQERAFANPKIEAVWNKLVVEVAGTERGT
                     DHLELEDTVTGTRSQLKVGGVFIFIGFKPNTGIVEGHVDHDAGGYLITDERMMTSIPG
                     LFAAGDVRSQLTRQVTTAVGDATTAAIAVEKYLAQLREQGRLSASAGAIVT"
     gene            1438..>1706
                     /locus_tag="AC16JC_06665"
     CDS             1438..>1706
                     /locus_tag="AC16JC_06665"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5164414.1"
                     /translation="MKVVQIPNGVFAENCYLVIDEASAECAVVDPGEEAGLILHKIEQ
                     EGVRAVGIWVTHAHLDHVMGVARVRAATGAAVWLHAADRPLYDRVS"
BASE COUNT          277 a          515 c          649 g          265 t
ORIGIN      
        1 gcagccccgg gaacggccca cgtactcgtg ggcacggtga gcgcctgcca cggcgccctg
       61 aatctgctgc ggatcggtgc gtcgcctgcc ggcggctgac ggctgatggc ttcgccgctg
      121 gatccccagg cgcgccggcg tcagctggaa caggcccggg cgctgttcaa cgacggggaa
      181 tactggctcg cccatgaggc cctggaaacc gtctggcgtt ctataataga cgaaggtgag
      241 cgtcgggtgt ggcaggggtt gatccaggcg gccgcggcgc tgctgcatcg gcggcgcggc
      301 aaccggcacg gcaccgtcgt cgtcggagcc gcggccctgg agaagctgtc cggaccgcag
      361 cacccggcgg tggagttcga tacgttgcgg tttcgcgccg tcctggcgcg tgccctggcg
      421 ggggagggcg atcccccgcg attggacttt cgaaacgatg cctgagagcg ggcagcagag
      481 ctacgacgtg attatcgtgg gcgccggtcc ggcggggctg tgcgcggcga tgtatgccgg
      541 ccgggggatg ctcaaggccc tgaccatcga gcggggcgcc cccgggggtg agctcctgaa
      601 caccgatctg gtggaagact acatcgggtt cgagagcatc aaggggtggg atctggccca
      661 gaagttcacc gaccatgcca agaagttcgg cgccgagatc gtgaccgata ccgtggagag
      721 cgtgcggcgg gccccggatg ggtggttcga tttgacgacc gcgcgcgagc ggcactaccg
      781 tgccccggcc gtgatcctca cggccggggg taccccggtg aaactcggcg taccggggga
      841 gcgggaatac gcgggcaaag gggtttccta ttgcgcggtg tgcgacgggg ccttcttcaa
      901 gggcgaggta ctggcggtgg tgggcggcgg tgacgccgcg gtcgaagagg cggactacct
      961 cacccgctac gcgtccaaag tgtacatcgt gcaccgccgc gaccggttcc gcgcgtcgag
     1021 gattctgcag gagcgggcct tcgcgaatcc gaagatcgag gcggtctgga acaagctggt
     1081 ggtggaggtc gccgggaccg agcgcggcac cgaccatctg gagctcgagg acacggtgac
     1141 ggggacccgc agccagctca aggtgggcgg cgtgttcatc ttcatcgggt tcaagccgaa
     1201 cacgggaatc gtggaaggcc acgtcgacca cgacgccggc gggtacctca tcaccgacga
     1261 gcgcatgatg acctcgatcc cgggcctgtt cgcggcgggc gacgtgcgca gtcagctgac
     1321 gcgccaggtc acgaccgcgg tcggggacgc cacgacggcg gcgatcgcgg tggagaagta
     1381 tctggcgcag ctgcgggagc agggcaggct ctccgcttcc gctggagcca tcgtcacgtg
     1441 aaggtggtcc agatccccaa cggggtcttc gccgagaact gctacctggt gatcgacgag
     1501 gcgtcggccg agtgcgccgt cgtggacccc ggcgaagaag cgggattgat tctccacaag
     1561 atcgaacagg aaggggtgcg ggcggtcggg atctgggtca cgcacgcgca tctggatcac
     1621 gtgatggggg tcgcacgggt gcgcgccgcc accggtgctg cggtgtggct gcatgcagcc
     1681 gaccggccac tgtatgaccg tgtgtc
//
LOCUS       DBNGZW010000278         2654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_842465, whole genome shotgun sequence.
ACCESSION   DBNGZW010000278 DBNGZW010000000
VERSION     DBNGZW010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_842465"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="AC16JC_06670"
     CDS             <1..449
                     /locus_tag="AC16JC_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5164415.1"
                     /translation="WRLMVDGLVEQPLSLSLDDLHRMPRASYTVKHHCVEGWSAIASW
                     AGVPVSEIVLRARPKPAARYILFRSFDSDYSNGWDMASAMHPQTILAYAMNDNPLPPE
                     HGAPLRLYSPTKLGYKMTKYLMSMTFMDTRPGGYWEDQGYPWFGGI"
     gene            complement(520..2568)
                     /locus_tag="AC16JC_06675"
     CDS             complement(520..2568)
                     /locus_tag="AC16JC_06675"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5164416.1"
                     /translation="MPDASATRGFGLAGRIFFASALLVVAVLGITFGITSFQANRTAD
                     AAIQRALGATRTAVEDFLAARIHTLGGLSTVATDVPQYRERLLKHGGERADALDQCND
                     VRDLIGAAWVLVADNSGLLIARTDYPDQLEVDFSGAPLVANALSGETSSGAWLDDVRN
                     KLFIAVGTPLRASPEAAPQGALVATFEVNDSLAQAIHQATSSDVVFFALDTLNRPYVV
                     GSTLPRDEMAAALQAGVKPEELAQDSTGEAMTAMSGGEHLIGLAGAVRSPAGDLRGGF
                     VAFRSREAELASFRTLQRTILAALLLGIVLALASAFVLARQIARPVQQLARATRRVQD
                     GDYSVEIPVTAQDEIGVLSQAFRNLVEDLKEKAQLVEYMMHAATGSTTQPLASAPTAV
                     RQLAERPSGDSSGDLLRLGSIFAGRYEVKDVLGSGGMGVVYRAFDRELGEPVAIKTLR
                     PDTVAGDPVALERFKQEIRLARKIAHRNVVRTYDLGEVDGQYYLTMEYVEGTSLKALI
                     ESRGRLPAPVTLTIGKQLCRALEVAHDQGVIHRDIKPQNIVVDPNGFLKVMDFGIARL
                     ANPPKGKGLTQAGMAIGTPDYMSPEQLSGMELDPRSDLYSTGVVLFECLTGRLPFEAD
                     TPWSLIAKHIEDVPPSPRSLNAEVPVALEQVVLRAMAKDREQRYQTAVELHDALEAIG
                     "
BASE COUNT          422 a          894 c          920 g          418 t
ORIGIN      
        1 gctggcgtct catggtggac ggcctggtcg agcagccgct ctccctgtcg ctcgacgacc
       61 tccaccgcat gccccgcgcg agctacacgg tgaagcatca ctgtgtggaa gggtggagcg
      121 cgatcgcgtc gtgggcgggt gtcccggtga gcgagatcgt gctgcgggcc cgccccaagc
      181 cggcggcgcg ctacatcctg ttccgcagct tcgattccga ttactccaac ggctgggaca
      241 tggcgagcgc gatgcacccg cagacgatcc tcgcatacgc catgaacgac aatccgctgc
      301 cgcccgagca cggggcgccg ctgcggctct actccccgac gaagctcggc tacaagatga
      361 cgaagtactt gatgtcgatg accttcatgg acacgcggcc gggcgggtac tgggaggacc
      421 aggggtatcc gtggttcggg gggatctaga aggtgccagg ggtcaggtgt cgggtggcag
      481 gtcataaggt cctgataccc ggaacccgac acctgacacc tagcctatag cttccagcgc
      541 atcgtgcagc tccaccgccg tctgatagcg ctgttcccgg tccttggcca tcgcgcgtag
      601 caccacctgc tcgagcgcca cgggtacctc ggcgttgagc gaccgcggac tgggcgggac
      661 atcctcgata tgcttggcga tgagcgacca gggcgtgtcg gcctcgaacg gcagccggcc
      721 ggtgaggcat tcgaacagca cgacgcccgt cgagtagagg tcgctccggg gatcgagctc
      781 cattccggag agctgctcgg gagacatgta gtcgggcgtg ccgatggcca tgcccgcctg
      841 ggtgaggccc ttccctttcg gcggattggc gagccgcgcg atcccgaaat ccatcacctt
      901 gaggaagccg ttgggatcga ccacgatgtt ctgcggcttg atgtcgcggt ggatgacccc
      961 ctggtcgtgg gccacttcga gcgcccggca caactgcttg ccgatggtca gggtgaccgg
     1021 ggcgggcagc cggccgcggc tctcgatcag cgccttcagc gacgtcccct cgacgtattc
     1081 catcgtgagg tagtactggc cgtcgacctc gcccaggtcg taggtccgga ccacgttgcg
     1141 gtgcgcgatc ttgcgggcga ggcggatctc ctgcttgaag cgctcgaggg cgacgggatc
     1201 accggcgacg gtgtcgggcc gcagcgtctt gatggcgacc ggctcgccga gctcgcggtc
     1261 gaacgcgcga tacaccacgc ccatgccgcc cgagcccagg acatccttca cctcgtagcg
     1321 gccggcgaag atcgagccga gacgcagcaa gtcgccactc gaatcgcccg agggccgctc
     1381 ggccagctgg cggacggccg tcggcgccga ggcgagcggc tgcgtcgtcg agcccgtagc
     1441 cgcgtgcatc atgtactcca ccagctgcgc cttctccttc aggtcctcca ccaggttgcg
     1501 gaacgcctgc gacagcacgc cgatctcgtc ctgcgcggtc accgggatct cgaccgaata
     1561 gtcgccgtcc tgcacacgtc gggtggcgcg ggcgagctgc tgcaccgggc gggcgatctg
     1621 ccgggccagc acgaacgccg aggcgagcgc gagcacgatg ccgagcagga gggccgccag
     1681 gatcgtccgc tgcagcgtgc ggaacgacgc cagctcggcc tcgcgcgagc ggaacgcgac
     1741 gaagccgccg cgcaggtcgc cggcgggcga gcggacggcg ccggccagtc cgatcagatg
     1801 ctcaccgccg gacattgccg tcatcgcttc ccccgtcgag tcctgcgcca gctcctcggg
     1861 cttcacaccg gcctgcaagg ccgctgccat ttcgtcgcga ggcagcgtgc tcccgacgac
     1921 gtatgggcga ttcagcgtgt cgagcgcgaa gaacacgacg tcggagctcg tggcctggtg
     1981 aatcgcctgg gccaggctgt cgttcacctc gaacgttgcg acgagcgcgc cctgcggcgc
     2041 cgcttccggt gaagctcgca gcggagtccc cacggcgatg aacagcttgt tccggacgtc
     2101 gtcgagccac gcgcccgagg acgtctcccc ggacagggcg ttggcgacga ggggcgcgcc
     2161 ggagaagtcg acctcgagct ggtcggggta gtcggtccgg gcgatcagga ggcccgaatt
     2221 gtccgcgacc agcacccagg cggcgccgat caggtcccgg acgtcgttgc actgatcgag
     2281 cgcgtccgcc cgctcgccac cgtgcttcag cagccgctcg cggtactggg gcacgtcggt
     2341 ggccaccgta ctgagcccgc ccagggtgtg gatacgcgcc gcgagaaaat cctccacggc
     2401 ggtccgcgtg gcgcccaggg cgcgctgaat cgccgcgtcg gccgtgcggt tcgcctggaa
     2461 ggaggtgatg ccgaaggtga taccgagcac ggccaccacg agcaacgcgg aggcgaagaa
     2521 gatccttccg gcgagaccga agcccctagt agcgctcgcg tccggcattc gtggggtagt
     2581 ccttgccgaa cttgttcttg tgcggctgcc agcggaagcc gcgcgcgttg agggtcacca
     2641 ccaggtcgga aact
//
LOCUS       DBNGZW010000279         1487 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_503673, whole genome shotgun sequence.
ACCESSION   DBNGZW010000279 DBNGZW010000000
VERSION     DBNGZW010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1487)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1487)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1487
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_503673"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(204..821)
                     /locus_tag="AC16JC_06680"
     CDS             complement(204..821)
                     /locus_tag="AC16JC_06680"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5164417.1"
                     /translation="MPLAAVVLAGGRSARMGTPKALLDFRSEPFVVRILEAVEALDLK
                     TRVVVIGPPPDGTAVRAAIAAHDCTVVENPDVDGGPIASLRRALDALETVHPPAVLVW
                     PVDLPHIHVVTIERLVEVYRRRHPPVTLPTFAARRGHPVIWDQSLFAELRSSEAATRE
                     GARAVLHAHMDQAVLVPVDDPAVTDFINTPADYERLIREINRDAY"
     gene            948..>1487
                     /locus_tag="AC16JC_06685"
     CDS             948..>1487
                     /locus_tag="AC16JC_06685"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR5164418.1"
                     /translation="MAVRRIRLLGDPMLRQRCQLVAKPTSGAARLVADDLRDTLKWAK
                     EKYGMGRALAAPQIGAPVRMIVMDVDGARQRTTLVNPEITDVGSEDFEVWDDCFSFPE
                     LLVRVNRAYRITIQYTDLKGNPHSLECEGPLAELLQHEIDHLDGILALDRAVGLDPFA
                     FRSEWEKLHDKSERYASPHP"
BASE COUNT          254 a          472 c          541 g          220 t
ORIGIN      
        1 gcagggcggc gtgcaggatc tcgacgcgcc gccgctggcg cttggcgcgc acgtgggcgg
       61 agccccgagt cggggggggc gcgtgggtac cgggtgctgc agcggccatg ccggagcctc
      121 gtgaggtggt ccggcaagaa aattgaaccg caattcaaaa atttatcgtg aagtcagtat
      181 gaagagcggc agccttccac acctcagtag gcgtcccggt tgatctcgcg gatcaggcgc
      241 tcgtagtcgg ccggggtgtt gatgaagtcg gtcacggccg ggtcatccac cggcacgagg
      301 acggcctggt ccatgtgtgc gtggagtacc gcccgcgcgc cctcgcgcgt cgcggcctcg
      361 ctcgagcgca gctccgcgaa caggctctgg tcccagatca ccgggtggcc ccggcgggcg
      421 gcgaatgtcg gcagcgtgac gggagggtgc cgccggcgat agacctcgac cagccgctcg
      481 atcgtcacca cgtgaatgtg ggggagatcc accggccaga cgaggacggc gggcggatga
      541 acggtttcga gcgcgtcgag ggcgcgacgc agcgaggcga tggggccgcc gtccacatcg
      601 gggttctcga ccacggtgca gtcgtgggcc gcgatcgccg cccggaccgc tgtcccgtcc
      661 gggggtgggc cgatcacgac cacgcgggtc ttcaggtcga gggcctcgac cgcctcgagg
      721 atgcggacca cgaagggctc gctgcggaag tcgagcagcg ccttgggcgt gcccattcgg
      781 gcggaacggc cgcccgccag gaccacggcg gcgaggggca tgaggagagc tacgataggg
      841 tggccgcgga gcggcggcaa accgggaaat cgtatgtgat tgcagaacaa tggcttgcaa
      901 ttgcactgtt gcgtcgcggc cgcaaaacat cagtattagg tccccccatg gccgtacgac
      961 gcattcgcct gctgggcgac ccgatgctgc gccagcgctg ccaactggtc gccaaaccca
     1021 cgtccggggc cgcccggctc gtggcggacg acctgcgcga cacgctcaaa tgggccaagg
     1081 agaagtacgg gatgggtcgg gccctggccg cgccgcagat cggcgcgccg gttcgaatga
     1141 tcgtgatgga cgtggatggc gcacgacagc ggacgaccct cgtcaacccg gagattaccg
     1201 acgtgggctc cgaggacttc gaggtgtggg acgactgctt ctcgttcccg gagctgctgg
     1261 tccgggtgaa ccgggcctat cgcatcacca tccagtacac ggacctcaag gggaaccccc
     1321 actccctgga gtgcgagggt ccgctggccg agctgctgca acacgagatc gatcacctgg
     1381 acgggattct ggcgctggat cgcgccgtcg gtctcgaccc gttcgcgttc cgcagcgaat
     1441 gggagaagct ccacgacaag agcgagcgct acgccagccc gcacccg
//
LOCUS       DBNGZW010000280         2318 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_427036, whole genome shotgun sequence.
ACCESSION   DBNGZW010000280 DBNGZW010000000
VERSION     DBNGZW010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_427036"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1991)
                     /locus_tag="AC16JC_06690"
     CDS             complement(<1..1991)
                     /locus_tag="AC16JC_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011686631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HIR5164419.1"
                     /translation="MNTLTLILATTVAAQTPTGAAPSPPDVARWERQARNVTIIRDDW
                     GIAHVYGKSDADAVFGMEYAQAEDDFNRVETNYINAMGRLAEAEGAEAVWQDLRMKLF
                     IDPDLMKAAYRSSPGWLKQLMDAFADGLNFYLYKHPEVKPRVITRFEPWMALTFSEGS
                     IGGDIERVNLRELAAFYGGDSAGPARTGDDEDSPEPGGSNGIAIAPAITRDHHALLWI
                     NPHTSFYFRSELQMVSEEGLDAYGAVTWGQFFVYQGFNRTAGWMHTSSGVDNIDEFLE
                     TVTQRGGRWTYRRGSQELPVSTRTITVPYRTATGTARRTFTVYFTRHGPVVRRIGDRW
                     VSVSLMQNPVRALTQSYSRTKAKDYTEFRRIMELHTNSSNNTVFADAAGNIAYFHSNY
                     IPRRDTSFDWTQPVDGSNPATDYRGVLAVDETPNLVNPPVGWLYNSNNWPWSAAGPSS
                     RRRDDYPRYVETGSEETPRGYHALRVLSDGKDWTMASLTAAAFDSYLPSFERMLPPLL
                     KAYDQLPDSDSVRLRLADQIAALRGWDYRWSAASIPTSLAVFWGGSVSQLVARDARAA
                     GISPQTYVAQRASPAELLGALAAATDALTADFGTWKTPWGAINRFQRFNDDIAPRFDD
                     AQPSIPVPFTSALWGSLASFGARPYPNTKQWYGTSGNSFV"
     gene            2072..>2318
                     /locus_tag="AC16JC_06695"
     CDS             2072..>2318
                     /locus_tag="AC16JC_06695"
                     /inference="COORDINATES: protein motif:HMM:NF015944.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HIR5164420.1"
                     /translation="MQWALVVGAVVLAWVILTFNSLTRLRQLSANAWSDIDVQLKRRH
                     DLIPAVVSVVKGHAGYERETFEALATDRARAIAASGTA"
BASE COUNT          341 a          751 c          829 g          397 t
ORIGIN      
        1 acgaagctgt tgccgctcgt accgtaccac tgcttcgtgt tggggtaggg ccgggcgccg
       61 aaggaggcca gcgaccccca cagggccgac gtgaagggga cggggatgct gggctgcgcg
      121 tcgtcgaagc gcggcgcgat atcgtcgttg aaccgctgga accggttgat ggcgccccag
      181 ggcgtcttcc aggttccgaa atccgccgtc agggcgtccg tggcggccgc cagcgcgccg
      241 agcagctccg ccggcgacgc gcgctgggcg acgtacgtct gcggcgatat ccccgccgcc
      301 cgggcgtccc tggccacgag ctgcgagacg ctcccccccc agaacaccgc cagggaagtg
      361 ggaatggacg cggcgctcca gcgataatcc cagccgcgca gcgccgcgat ctggtccgcc
      421 aggcgtagcc ggaccgagtc gctgtccggc agctgatcat aggccttgag gagcggtggg
      481 agcatccgct cgaacgaggg caggtagctg tcgaaggccg cggcggtgag gctggccatc
      541 gtccagtcct tcccgtcgct gagcacgcgg agcgcgtggt agccgcgcgg cgtctcctcg
      601 ctgccggtct cgacgtagcg agggtagtcg tcgcgccggc ggctggaggg gcccgccgcc
      661 gaccagggcc agttgttgga gttgtagagc catccgaccg gcgggttcac gaggttcggc
      721 gtctcgtcca cggcgagcac gccgcggtag tcggtcgcgg ggttgctgcc gtccaccggc
      781 tgcgtccagt cgaacgacgt atcgcggcgc gggatgtagt tcgagtggaa gtacgcgatg
      841 ttccccgcgg catcggcaaa cacggtgttg ttggatgaat tggtgtgcag ctccatgatg
      901 cggcggaact cggtgtagtc cttcgccttg gtgcggctgt aggactgggt gagcgcccgc
      961 accgggttct gcatcaggct cacgctgacc cagcggtccc cgatccggcg gacgacgggc
     1021 ccgtgccgcg tgaaatagac ggtgaaggtc cggcgggcgg tgccggtggc cgtccgatac
     1081 ggcacggtga tcgtgcgcgt cgagaccggc agctcctggc tgccgcggcg gtacgtccag
     1141 cggccacccc gttgcgtcac cgtttccagg aactcgtcga tgttgtccac gccgctcgag
     1201 gtgtgcatcc agccggcggt gcggttgaac ccctgataga cgaagaactg cccccaggtc
     1261 accgcgccgt aggcgtcgag tccctcctcg ctcaccatct ggagctcgga gcgaaaataa
     1321 aacgacgtgt gcgggttgat ccacagaagg gcgtggtggt cgcgcgtgat cgcgggcgcg
     1381 atggcgatgc cgttggaacc gccgggctcg ggggagtcct cgtcgtcgcc ggtccgggcc
     1441 gggccggccg agtcgccacc gtagaacgcc gcgagctccc gcagattgac ccgttcgatg
     1501 tctcccccaa tgctgccctc gctgaacgtg agcgccatcc acggctcgaa gcgggtgatg
     1561 acccgtggtt tcacctcggg atgcttgtag agatagaagt tcaggccgtc ggcgaaggcg
     1621 tccatcagtt gcttcagcca ccccgggctc gagcggtacg ccgccttcat caggtcgggg
     1681 tcgatgaaca gcttcatgcg cagatcctgc cagacggcct ccgctccttc ggcctccgcc
     1741 agccgtccca tcgcattgat gtaattggtc tccacgcggt tgaagtcgtc ctcggcctgg
     1801 gcgtattcca tgccgaagac ggcgtcggcg tcggacttgc cgtacacgtg ggcgatgccc
     1861 cagtcgtcgc gaatgatggt gacgttgcgg gcctgccgct cccagcgggc gacgtccggg
     1921 ggggaaggcg ccgctcccgt tggggtctgc gcggccaccg tggtggcgag gatgagcgtg
     1981 agcgtgttca tggtgctcaa ctctatcgtc ccggcggcgc ggggtccatc ctatagtgtt
     2041 ccgcaccgcg acctttccgg agaatgctcg cgtgcaatgg gctctcgtcg tcggcgccgt
     2101 cgtcctcgcc tgggtgatcc tcacgttcaa cagtctgacg cgactgcggc agctgagcgc
     2161 caacgcgtgg tcggacatcg acgtccagct caagcgccgc cacgatctca ttccggcggt
     2221 ggtgtcggtg gtgaaggggc acgccggcta cgagcgggag acgttcgagg cgctcgcgac
     2281 agaccgcgcc agggccatcg cggcgagcgg cacggcag
//
LOCUS       DBNGZW010000281         3901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_637121, whole genome shotgun sequence.
ACCESSION   DBNGZW010000281 DBNGZW010000000
VERSION     DBNGZW010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3901
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_637121"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1147
                     /locus_tag="AC16JC_06700"
     CDS             26..1147
                     /locus_tag="AC16JC_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011418845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR5164421.1"
                     /translation="MALPRLRLFSFRGIPIRLDASWFAIAVLVTWSLAAQLFPAWHAG
                     LPRTTYWVMGIVGALGLFASIVVHELSHALIARRHDIPIEGITLFVFGGVAEMGAEPS
                     SPKAELNMAIAGPLTSIVIGAAALAASALGSGWPAAVVTVLAYLGVINLLLAAFNLVP
                     AFPLDGGRVLRALLWSRHGSVVRATHTASRVGTGFSVVLMVLGGLRVLAGDLLGGVWF
                     FLIGMFLRQAADASYQQVVVRRALEHEPVRRFMTTGPVSVRPDLTLAEFLDSYVYRYH
                     HKLFPVQDNGRLIGQISTEQLHQVPRPDWPSRTVGAVIVPLAEAVTVTPDTAAMQALG
                     RMRQTGRSRLLVVDDGRLAGILSARDLLDFLALKLELEP"
     gene            1144..1851
                     /locus_tag="AC16JC_06705"
     CDS             1144..1851
                     /locus_tag="AC16JC_06705"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR5164422.1"
                     /translation="MTTPFTGPLADHIAEPVHVPIGGDRRLPGTLALPPHPAGIVLFA
                     HGSGSSRLSPRNQVVADRLFAANVGWLLFDLLTETEAASRSAVFDIPLLAERLRQATT
                     WVAEYPATRDLAPGYFGASTGAAAALVAAAGNPRIHAVVSRGGRPDLAGAALRQVLAP
                     TLLIVGGADTGVLELNAQALRELGGVKEMIVVPHATHLFEEPGTLEAVAGHAADWFRR
                     YLQTAEVEHLASSGRAS"
     gene            1848..2126
                     /locus_tag="AC16JC_06710"
     CDS             1848..2126
                     /locus_tag="AC16JC_06710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164423.1"
                     /translation="MKSRMQRMKRTAARAARTTKRVAGKIAKRTRKVLKRVGKQARSR
                     ETRRQVARVLQGTGGVLETAGKAVVIAGAAAEVAAEELREGPTVSRPA"
     gene            complement(2104..2646)
                     /gene="hpt"
                     /locus_tag="AC16JC_06715"
     CDS             complement(2104..2646)
                     /gene="hpt"
                     /locus_tag="AC16JC_06715"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR5164424.1"
                     /translation="MRTSDAPPALISERRIAERVEELARQISADYAEVDELLLVGVLR
                     GAFIFLADLARRLDVPCSIDFMALSHYEHGSTPSAGVRLIMDLRVPIAGRHVLLVEDI
                     VDSGETLAYLRRMLEARRPASLKICTLTRKPDRLTSEVRLDYVGFDIPDAWVVGYGLD
                     YDNRFRALPYIGMLKPAAKP"
     gene            2729..3652
                     /locus_tag="AC16JC_06720"
     CDS             2729..3652
                     /locus_tag="AC16JC_06720"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5164425.1"
                     /translation="MSWKPIIVGVDGSPAAAAAAQVGSQIAATAGTECRLVHAARDNW
                     SMLAAAQVPQQAGAFRDTALAAARTQITTALADRVPAETLRGLVVREGWPPAVLDDVA
                     READASLVVLGAKHHTALGRWLGGSTVHNVARMLRAPLLVVGSPRAAIRRILVPLDAS
                     HAAQPTLDLAGRIADLFDAELRVLHVIEPLPILPEVPPPTNPADYERLLTEECEQKIW
                     PLVPADASRIIRHTAADAGIAAEATAWQADMVVVGSHGKGVVERMLVGSVTERLLGHL
                     PAPMLLVVPVARLAPTPHPVTTAKLRMATVA"
     gene            complement(3712..>3901)
                     /locus_tag="AC16JC_06725"
     CDS             complement(3712..>3901)
                     /locus_tag="AC16JC_06725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164426.1"
                     /translation="GKGIVERMLVGSVTERLLGHLPAPMLLVVPVPRLAPTPQPAITA
                     RYGWVVRIAQDGEQVSIT"
BASE COUNT          539 a         1346 c         1413 g          603 t
ORIGIN      
        1 ggggtaggct gggcacggag ggcatatggc gctccctcgc ctgcgtctgt tcagtttccg
       61 tggcatcccc attcggctcg atgcgagctg gttcgcgatc gcggtcctgg tgacctggtc
      121 gctcgccgcg cagctcttcc cggcgtggca cgccggcctg ccgcgcacga cctactgggt
      181 gatgggcatc gtcggcgcgc tggggctgtt cgcctccatc gtggtgcacg agctgtccca
      241 cgcgttgatc gcgcgccgcc atgacattcc catcgagggg atcacgctct tcgtcttcgg
      301 cggggtggcg gagatgggtg cggagccgtc gagcccgaag gcagagctga acatggcgat
      361 cgctgggccg ttgacgagca tcgtcatcgg cgctgcggcg ctcgccgcgt cggccctggg
      421 gagcggatgg cccgcggcgg tcgtgacggt gctcgcctac ctcggagtga tcaacctgct
      481 gctcgcagcg ttcaacctcg tccctgcgtt cccgttggac ggcggccgcg tgctgcgggc
      541 cctgctctgg agccgccacg ggagcgtcgt gcgagccact catacggcgt cgcgggtcgg
      601 gaccgggttc agtgtcgtac tgatggtgct gggcggcctc cgcgtgctcg cgggggatct
      661 gctcggcggc gtgtggttct tcctcatcgg catgttcctg cgacaggccg ccgacgcatc
      721 ctaccagcag gtggtggtgc gccgcgcgct cgaacacgag ccggtgcgcc ggttcatgac
      781 caccgggccg gtctccgtgc gcccggatct cacgctcgcc gagttcctgg actcctacgt
      841 ctatcgctac caccacaagc tctttcccgt ccaggacaac ggccgtctga tcgggcagat
      901 ctccaccgaa cagctgcacc aggtgccgcg cccggactgg ccgtcgcgca ccgtgggcgc
      961 cgtgatcgtt ccgctggccg aggccgtgac ggtcacgccc gacacggccg cgatgcaggc
     1021 gctggggcgc atgcgccaga ccggccgctc ccgactgctg gtcgtggacg acgggcggtt
     1081 ggccggcatc ctctcggcgc gtgatctcct ggacttcctc gcgctcaaac tggagctgga
     1141 gccatgacga caccgttcac cgggccgctc gccgaccaca tcgcggagcc cgtccacgtc
     1201 cccatcggcg gggatcgccg gctgccggga acgctcgccc tgccgccgca tcccgccggc
     1261 atcgtgctgt tcgcccacgg cagcggcagc tcgcggctgt cgccccgcaa tcaggtcgtc
     1321 gcggatcggc tgtttgcggc gaacgtcgga tggctgctgt tcgatctgct caccgagacg
     1381 gaggccgcat ctcgttcggc ggtgttcgac atcccgctgc tcgccgagcg gctgcgtcag
     1441 gcgacgacgt gggtagcgga gtatccggcc acccgcgacc tcgcccccgg ctatttcggg
     1501 gccagcaccg gcgcggcggc ggcgctcgtg gcggcggccg gcaatccgcg catccacgcg
     1561 gtcgtgtccc ggggcgggcg gcccgatctc gcgggcgcgg cgttgcgcca ggtcctcgcg
     1621 cccacgctgc tgatcgtggg gggcgccgat acgggcgtgc tcgagctgaa cgcgcaggcg
     1681 ctgcgcgagc tgggcggcgt gaaggagatg atcgtcgtgc cgcacgccac ccatctgttc
     1741 gaagagcccg gcacgctcga ggcggtggcg ggtcatgcgg cagactggtt ccgccgctac
     1801 ttgcagacgg ccgaggtcga gcacctcgcg tcgtcgggga gggcatcatg aagtcgcgta
     1861 tgcaacggat gaaacggact gccgcccggg ccgctcgcac gacgaagagg gtggccggga
     1921 agatcgccaa gcgcacgcgc aaggtgctga agcgggtcgg caagcaggcc cgctcccgcg
     1981 agacgcgccg ccaggtggcg cgcgtgctcc aggggaccgg tggggtgctg gagaccgccg
     2041 gcaaggcggt ggtgatcgcg ggagccgcgg cagaagtcgc cgccgaagag ctgcgcgagg
     2101 ggcctacggt ttcgcggccg gcttgagcat gccgatgtat gggagcgccc gaaaccgatt
     2161 gtcgtagtcc aggccgtagc cgacgaccca cgcgtccggg atgtcgaagc ccacgtaatc
     2221 gagccggacc tccgacgtca ggcggtccgg cttgcgcgtc agcgtgcaga tcttgagcga
     2281 ggcgggacgt cgggcttcca gcatgcgccg caggtaagcg agggtctccc ccgaatcgac
     2341 gatgtcctcc accaggagca cgtgccgtcc cgcgatcggt acgcgcaggt ccatgatcag
     2401 gcgcacgccg gcgctcggcg tcgaaccgtg ctcgtagtgc gacagggcca tgaagtcgat
     2461 gctacagggg acgtcgagcc ggcgggccag gtcggcgaga aagatgaacg ctccgcgcag
     2521 cacgccgacc aggagcaact cgtcgacctc cgcgtaatcg gcggagatct ggcgcgccag
     2581 ctcctcgacg cgctcggcga tgcggcgctc ggaaatgagc gccggaggcg catcggatgt
     2641 ccgcactcca aagcctcctt ctgccggtcc tcaccgttcc ttcatgaagg gtggggcaac
     2701 ttgcgcttga aagcaaactg gggggcatat gtcctggaag ccgatcatcg tgggggtgga
     2761 tggctcgccg gccgccgccg cagcggccca ggtgggatcg cagatcgcgg cgactgcggg
     2821 gacggagtgc cgtctggtgc acgcggcgcg tgacaactgg agcatgcttg ccgccgccca
     2881 agtcccgcag caggccggcg cgttccgcga cacggcactg gccgcggcgc ggacacagat
     2941 caccacggcc ctggccgacc gggtgccggc cgagaccctg cgcgggctcg tcgtgcgtga
     3001 gggatggccc ccggcggtgc tcgacgatgt cgcccgggaa gcggatgcga gcctggtcgt
     3061 gctcggcgcc aagcaccaca cggcgctcgg tcgctggttg ggcggcagca ccgtgcacaa
     3121 cgtcgcccgc atgctgcgcg ccccgttgct cgtcgtcggg tcgccccggg cggcgatccg
     3181 gcgaatcctc gtgccgctcg atgcgtccca cgctgcgcag cccacgctcg acctcgccgg
     3241 ccggatcgcg gatctgttcg atgccgaact gcgggtgctg cacgtcatcg agccgctgcc
     3301 gattctgccc gaggtgccgc cgccgaccaa tcccgcggac tacgagcggc tgctcaccga
     3361 ggagtgcgag cagaagatct ggccgctggt cccggcggac gcgagtcgca tcatccgcca
     3421 caccgccgcg gatgccggga tcgccgccga ggcgacggcg tggcaggccg acatggtggt
     3481 cgtgggctcg cacggcaagg gggtcgtgga gcggatgctc gtcggcagcg tcaccgagcg
     3541 gctgctcggt catctcccgg cgccgatgtt gctcgtcgtc ccggtcgcca gactggcgcc
     3601 gacgccgcac ccagtcacta cggcaaagtt gaggatggcc accgtcgcct aggacgggga
     3661 gtcgcgtcgt tgcagggggc ccggcggcaa cgccgggccc cctggcgttc atcaggtgat
     3721 ggagacctgc tcgccgtcct gcgcgatgcg caccacccaa ccgtagcgcg ccgtgatcgc
     3781 cggctgcggc gtcggcgcca gcctggggac cgggacgacg agcagcatcg gagccgggag
     3841 atggccgagc agccgttcgg tgacgctccc gacgagcatc cgctctacga tccccttgcc
     3901 g
//
LOCUS       DBNGZW010000282         8165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1186026, whole genome shotgun sequence.
ACCESSION   DBNGZW010000282 DBNGZW010000000
VERSION     DBNGZW010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8165
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1186026"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..534
                     /locus_tag="AC16JC_06730"
     CDS             <1..534
                     /locus_tag="AC16JC_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HIR5164427.1"
                     /translation="REVNAGHDGTWVAHPGLVPVARGVFDARMAASHQLQVTRADVRV
                     TAADLLAVPEGEITFGGLRTNIDVGIQYLEAWLRGTGCVPIYNLMEDAATAEISRTQV
                     WQWVTHGAKLADGRRITADLVRRTMTHELGAMRDQLGPTRYDSGKFDLASKLFDQMMT
                     GTDFPEFLTTVAYDYLD"
     gene            551..1849
                     /gene="aceA"
                     /locus_tag="AC16JC_06735"
     CDS             551..1849
                     /gene="aceA"
                     /locus_tag="AC16JC_06735"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019380121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HIR5164428.1"
                     /translation="MPKQQFITDLERHWATSPRWTGITRPYSAEDVWRLRGSLGITHT
                     LAEVGAEKLWRLLHEEPHIAALSAVSGGQAVQQVKAGLKAIYISGWQVAGDMNTSLET
                     YPDPSLYPADSVPRLVERINNALLRADELDHMNGRTEIDWIVPLVADAEAGFGGLLNA
                     YELMKHMIRKGAAGVHFEDQLASLKKCGHLGGKVLVPTSEFITKLVAARLAADVLDVP
                     TVLIARTDALSATILANDIDPYDREFVIGDRTPEGFVAVQPGIRQVIARALAYAPYAD
                     LLWFETSKPDMDEARAFAEAVHEKFPGKMMAYNCSPSFNWKRHLSDEQIAGFQRELGA
                     MGYKFQFVTLSAFHAMNYAMFELARGYKDRGMSAYSELQQREFAAEQDGYEAVRHQEF
                     VGVPYFDQITEIVTGGRSSSIAMKGSTEEEQFHEPARATS"
     gene            2402..2941
                     /locus_tag="AC16JC_06740"
     CDS             2402..2941
                     /locus_tag="AC16JC_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164429.1"
                     /translation="MVDIKHRPPLDAVLRTAELLLAARHHERAGRLREAIDCCMAAIA
                     EAAWCGAWAAQAVGLRRLGALHHHRNELTAARESWRTSVEVAMRAGETAIAAEALISI
                     GNVELALGNVPAARDAYREGLAALGDDHHALRARIERDLASLPNTDPRPQSEPARVTA
                     TGAWAERSASPPVPAPVPA"
     gene            complement(2880..4724)
                     /locus_tag="AC16JC_06745"
     CDS             complement(2880..4724)
                     /locus_tag="AC16JC_06745"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5164430.1"
                     /translation="MAEAKRRTISSEAWRDARALIWAHRRRLTLGLVLLVANRLTGLV
                     LPSSSKLVVDRVIGQQQPELLVWIAVAVGVATLVQAATSFALSQVLGVAAQRAITDMR
                     RSVQEYVLRLPTSYFDSTKTGVLISRVMNDAEGIRNLVGTGLVQLVGGLITAVIALGV
                     LFYLNWHLTVLSILVLGVFGGGLAYAFTKLRPLFRERSKINAEVTGRLAETLGGIRIV
                     KAYHAERAERLVFTKGVNKLFRNIAATMTGISAVGGFSALVIGVIGVIMILVGGHAVV
                     ARTMTLGDLVMYLLFTGMMAAPVIQIASIGTQITEAFAGIDRIREVRSQVTEDAGDAA
                     RQPLGEVRGAVEFEDVTFAYKPGVDVLKQVSFRAPAGSTTALVGSSGSGKSTLIGLVM
                     AFNRPRAGRVLVDGRDLATVPLRDYRTQLGVVLQDNFLFDGTIADNIRFARPHASSAE
                     IQAVARIAHCDQFIGEFEHGYDTVVGERGVKLSGGQRQRVAIARAILADPRILILDEA
                     TSSLDSESEALIQDGLRSLRRGRTTFVIAHRLSTIQSADQILVLEGGEIVERGSHAQL
                     LAAGGRYRQLYDKQYRFEVDRFINPGEDFTPEPEQVPVATPIAPPTRL"
     gene            4784..5053
                     /locus_tag="AC16JC_06750"
     CDS             4784..5053
                     /locus_tag="AC16JC_06750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164431.1"
                     /translation="MKRSTLITLIVVVAIAGLFFYMTTARASTTCQVCVSYGGRSNCA
                     SAVGPTEQDAREGAQTTACGPIAAGMDASIACGRVEPVSVQCSTR"
     gene            5055..5519
                     /locus_tag="AC16JC_06755"
     CDS             5055..5519
                     /locus_tag="AC16JC_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164432.1"
                     /translation="MTTFHLPRTTVPARITLTQGRPRGGTIFLGARVPQHDGPETPLE
                     MLNRAEPFFPFQPEEGEILLVAKGHMIELTVEHGPIADPDRLSAAKLLRLELVLANGA
                     TLTGQATVELPEHHSRPLDYLNANAEPFFTLTTDESTHYVNRAHVLYALPRD"
     gene            5523..7160
                     /locus_tag="AC16JC_06760"
     CDS             5523..7160
                     /locus_tag="AC16JC_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002620768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR5164433.1"
                     /translation="MTARLDPFVDVLLREQADQLYLLPDEPVTIVKDGKPRKVSKQPL
                     TDAHIYALLAEIAPSDSADKIDRQAETEFEYVADRGLVRFRIVPETGRLTAVISPTAR
                     PARSTPQPAPLGGVERRRSQGAAAPPKAPPPPPPPPRPSAIAAAPLIESPVAPPEPPP
                     RAALPGEFAGDQYRAAEARLVALLKQLVGSESSDLHLRVGEPPVFRTHGQIARQPGPP
                     LTSDELALMLLAIMPERNRGEWKENGDSDFAYEIPGLARFRVNAGRDRKGPIAVFRVI
                     PAKVVTVEELGLGKDLTQLCFLNKGLVLVSGPTGSGKSTTLCALIDFINRERTDHVVT
                     IEDPIEFVHENKRCLITQRQVHLHTESFKSALRAALREDPDIVLVGEMRDLETIAIAI
                     ETAETGHLVFGTLHTTTAASTVDRIIDQFPADRQAQIRVMLSESLRGVICQTLCRKIG
                     GGRVAAREVLLSTPAVSNLIREGKTYQIPSIIQTSRKAGMITLNDALLDLVEKRQIEP
                     DEAYLRSVEKAPMLQALRAKGHAVTVGAAPGERPATG"
     gene            complement(7150..7572)
                     /locus_tag="AC16JC_06765"
     CDS             complement(7150..7572)
                     /locus_tag="AC16JC_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164434.1"
                     /translation="MRRARLFVTGIGALILAGCPSATAPHQVGIGGGGGGGRILAFIA
                     QPVTSHVFPAFINTVQVEVRDSITGVRDTTATGGVTLALGSNPGVTSLSGTTTVSFVS
                     GVSTFTDLSINVVASGYTITAASSGLTTITSQSFDIIP"
     gene            7672..>8165
                     /locus_tag="AC16JC_06770"
     CDS             7672..>8165
                     /locus_tag="AC16JC_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017298922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5164435.1"
                     /translation="MALRTQAPEGDHPGSARAADPIPNYIGGAWVPSRATHALPLINP
                     ATGETLAQVPLAGKADVDAAVQAAQDAFPRWRATPPVVRARHLFTLKGLLETHFDELA
                     AIVTRENGKTLDESRGSIRRAIENVEHACGIPTLLMGDALEDVATGIDCEYVRQPMGV
                     FAAIT"
BASE COUNT         1390 a         2855 c         2730 g         1190 t
ORIGIN      
        1 cgggaggtca atgccgggca cgacggcacc tgggttgcgc atccagggct ggtgccggtg
       61 gccagaggcg tcttcgacgc caggatggcg gcctcccacc aactgcaggt gacgcgcgcc
      121 gacgtgcgcg tcaccgccgc ggacctgctc gccgtgccgg agggagagat caccttcggc
      181 gggctgcgga ccaacatcga cgtgggcatc cagtacctcg aagcctggct gcggggcacc
      241 ggatgcgtcc cgatctacaa tctgatggaa gacgcggcca cggcggagat ctcccgcacg
      301 caggtgtggc aatgggtgac gcacggcgcg aagcttgcgg acgggcggcg aatcacggcc
      361 gatctggtcc gccggacgat gacacacgag ctcggagcca tgcgcgacca gctgggcccg
      421 actcggtacg actcggggaa gttcgatctt gcgtcgaaac tcttcgacca gatgatgacg
      481 gggacggact tcccggaatt cctgacaact gtggcctatg actatctcga ctagccctcg
      541 gaggggacgc atgccgaagc aacagttcat caccgacctg gagcgccact gggccacgag
      601 cccgcgttgg accggaatca cccggcccta ctccgcggaa gacgtgtggc ggctcagggg
      661 atcgctcggc atcacccaca cgctcgcgga agtgggggcc gagaagctgt ggcgcctgtt
      721 gcacgaggag ccgcacatcg ccgccctgtc ggctgtgagc ggcgggcagg cggtgcagca
      781 ggtgaaggcc ggcctcaagg cgatctacat ctccggctgg caggtcgccg gagacatgaa
      841 cacctcgctc gagacctatc ccgacccgag cctctatccc gccgacagcg tgccgcggct
      901 ggtcgagcgc atcaacaacg ccctgctgcg ggccgacgag ctcgaccaca tgaacgggcg
      961 taccgagatc gactggatcg tgccgctggt ggccgatgcc gaagccggat ttggcgggct
     1021 gctcaacgcc tacgagctga tgaagcacat gatccggaag ggcgcggcgg gcgtgcactt
     1081 cgaggaccag ctcgcctcgc tcaagaagtg cgggcacctg ggcggcaagg tactggtgcc
     1141 cacgagcgag ttcatcacga agttggtggc ggcgcggctc gccgcggacg tgctggacgt
     1201 gccgaccgtg ctcatcgccc ggaccgatgc cctgagcgcc accatcctgg ccaacgacat
     1261 cgatccctac gaccgcgaat tcgtgatcgg cgaccgcacg ccggagggat tcgtagccgt
     1321 gcaacccggg atccggcagg tcatcgcgcg cgccctggcc tacgcgccct atgccgatct
     1381 cctgtggttc gagacgagca aacccgacat ggacgaagcc cgggcgtttg ccgaggcggt
     1441 gcacgagaaa ttccccggca agatgatggc ctacaactgc tcgccgtcct tcaactggaa
     1501 gcgccacctg tccgacgagc agatcgcggg gttccagcgc gagctcggag cgatgggata
     1561 caagttccaa ttcgtgaccc tgtcggcctt ccacgcgatg aactacgcca tgttcgagct
     1621 ggcccgcggc tacaaggacc gcggcatgtc cgcctactcc gagctgcagc agcgggaatt
     1681 cgccgcggag caggacggct acgaggccgt gcggcaccag gagttcgtgg gcgtgccgta
     1741 cttcgaccag atcaccgaga tcgtgacggg cggccgctct tcgtccatcg ccatgaaggg
     1801 atcgaccgag gaagaacagt tccacgagcc cgcgcgtgct acgagttgag gcaatgcaac
     1861 accgcggcgt gcccctgtgg tgtgcgacga cgacgctctt cggccgccgc gccggcggcg
     1921 tgccatcgtg gaccgggcta actcgcggtt ccgctgcatg ttacgatggg cgcacacgct
     1981 gggagcgcgt ggcccactag ttgctgaggg aatgggcagc gattttgggg aggatttctc
     2041 gccggcaaag gcggcgtctc caggcaccgc gccggcggtc gggtgccgtg cacggcaccc
     2101 gacacctccc tccgagtatc gggccgttcg gacatagggg cggcggggag cgacggatag
     2161 caggaccggg aaacgccctc tccgctcgcc tcgtccggcg ggcggcacca gcatagaggc
     2221 agcgcttcgg cgccacgcct caatcacgcg aagcgccggg gacccgaaat ccccggcgtt
     2281 ttgcgcacca ggggaggcat ctggcccgct gccctgtgcc ccccgccaca gactggcatc
     2341 ctgcttgcat gcgaacgtac gcacagttac aagggtttaa cgcccgactc cgcgaggtaa
     2401 cgtggtagac atcaaacacc gaccgccgct ggatgcggta ctccggaccg cggagctcct
     2461 cctggccgca cggcatcacg aacgggcagg ccggctgcgc gaggcgatcg attgttgcat
     2521 ggccgcgatc gcggaagccg cctggtgcgg agcgtgggcg gcacaggcag tcgggttacg
     2581 gcggctgggg gcgctgcacc accaccgcaa cgagctgacg gcggcccgcg agagctggcg
     2641 caccagcgtc gaggtggcga tgcgggccgg ggagacggcg atcgcggcgg aagcgctcat
     2701 ctccatcggc aacgtcgagc tcgcgctcgg gaacgtcccg gcggcgcgtg atgcctaccg
     2761 cgagggtctg gcggcgctgg gcgacgacca ccacgcgctc cgcgcccgga tcgaacgcga
     2821 cctggcgagc ctgcccaaca cggacccgcg gccccagagc gagccggcgc gcgtgaccgc
     2881 tacaggcgcg tgggcggagc gatcggcgtc gccaccggta cctgctccgg ttccggcgta
     2941 aagtcctcgc ccggattgat gaaccggtcg acctcgaagc gatactgctt gtcgtagagc
     3001 tggcgatacc gcccgccggc ggcgagcagc tgggcatgcg agccgcgctc cacgatctct
     3061 cccccctcga gcaccaggat ctgatccgca ctctggatgg tggagagccg gtgggcgatc
     3121 acgaacgtcg tccggccgcg ccgcaggctc ctgagcccat cctggatcag ggcctcgctc
     3181 tcggaatcga gactcgaggt ggcttcgtcg agaatcagga tgcgcgggtc ggccaggatg
     3241 gcgcgcgcga tcgccacccg ctggcgctgg cccccggaca atttcacgcc ccgctctccc
     3301 accaccgtgt cgtacccgtg ctcgaattcc ccgatgaact gatcgcagtg tgcgatgcgg
     3361 gcgacggcct ggatctcggc gctggacgca tgcggccggg cgaagcggat gttgtccgcg
     3421 atggtcccat cgaagaggaa attgtcctgt agcaccacac cgagctgcgt gcggtaatcc
     3481 cgcagcggca cggtggcgag gtcgcggccg tccaccagca cccgccccgc ccgcggccgg
     3541 ttgaaagcca tcaccagccc gatcagcgtg ctcttgccgg agcccgagga ccccaccagc
     3601 gccgtggtcg agccggcagg cgcgcggaag ctcacctgct tgagcacgtc caccccgggc
     3661 ttgtaggcga aggtcacgtc ctcgaattcg accgcgccgc ggacttcacc cagcggctgc
     3721 cgggcggcgt cgccggcgtc ctcggtgacc tgggatcgga cctcgcggat ccggtcgatc
     3781 cccgcgaacg cctcggtgat ctgcgtcccg atggaggcga tctgaatgac cggcgccgcc
     3841 atcatcccgg tgaacagcag gtacatcacc aggtcgccca gcgtcatcgt cctggccacc
     3901 accgcatgcc cgcccaccag gatcatgatc acgccgatca ccccgatcac cagggcggag
     3961 aacccgccca ccgcggagat cccggtcatg gtagcggcga tgttgcggaa cagcttgttc
     4021 acgcccttcg tgaacaccag gcgctccgcc cgctcggcgt gataggcctt cacgatgcgg
     4081 atcccgccca gggtctccgc caggcggccg gtgacctcgg cgttgatctt cgagcgctcc
     4141 cgaaacagcg gccgcagctt cgtaaacgca taggccaggc cgccgccgaa cacgccgagc
     4201 accaggatgg acagtaccgt cagatgccag ttcagataga acaggacgcc gagcgcgatg
     4261 acggccgtga tcaatccgcc caccagctgc accagccccg tcccgaccag gttccggatc
     4321 ccctcggcat cgttcatcac gcgcgagatc agaacgccgg tcttggtgga gtcgaagtag
     4381 ctggtcggca ggcgcagcac gtattcctgg acgctgcggc gcatgtcggt gatcgcccgc
     4441 tgggcggcca cgccgagcac ctgggagagg gcgaacgagg tcgccgcctg aaccagcgtg
     4501 gccacgccca ccgccacggc gatccacacg aggagctcgg gctgctgttg tccgatcacc
     4561 cggtcgacca ccagtttgga cgacgacggg aggacgagcc ccgtcagccg gttcgcgacc
     4621 agcaggacga ggcccagggt cagccgccgg cggtgggccc agatgagggc ccgcgcgtcg
     4681 cgccaggcct cggagctgat ggtgcgcctt ttcgcttccg ccataccgga tgttagcttg
     4741 ggtgcggacc gcccgtccac ccgctccacc acgcgaggcc ctcgtgaaac gttccacgct
     4801 gattacgttg atcgtcgtcg tcgccatcgc cggcctcttc ttctatatga cgacggctcg
     4861 cgcctccacc acctgccagg tctgcgtcag ttacggcggc cgcagcaatt gcgcgtccgc
     4921 cgtcggtccc accgaacagg acgcccggga aggcgcgcag acgacggcct gcggaccgat
     4981 cgccgccggc atggacgcgt ccatcgcctg cggccgcgtc gagcccgtct cggtgcagtg
     5041 cagcacccgc tgacatgacg acgttccatc tgccccggac gacggtgccc gcccggatca
     5101 cgctcaccca gggccggccg cgcggcggca cgatcttcct gggagcgcgg gtgccccagc
     5161 acgacggccc ggagacgccc ctggaaatgc tgaaccgcgc cgagccgttt ttcccgttcc
     5221 aacccgagga gggggaaatc ctcctcgtgg ccaaggggca catgatcgag ctcacggtcg
     5281 agcacggacc catcgccgat cccgatcgcc tgtcggcggc caaactgctg cggctggagc
     5341 tggtcctcgc caacggcgcc acgctcaccg gacaggccac ggtggagctg ccggagcacc
     5401 actcgcggcc cctcgactac ctgaacgcca acgcggagcc gttcttcacc ctcacgaccg
     5461 acgagagcac ccactacgtc aaccgggccc acgtgctgta cgccctcccc cgggactgag
     5521 cgatgaccgc gcgcctcgat cccttcgtcg acgtcctgct gcgcgagcag gccgaccagc
     5581 tgtatctcct ccccgacgag cccgtcacca tcgtcaagga cggcaagccg cggaaggtca
     5641 gcaaacagcc gctcaccgat gcccacatct acgcgctgct ggcggagatc gccccgtcgg
     5701 actcggcgga caagatcgac cggcaggcgg agacggagtt cgaatatgtg gccgaccgcg
     5761 gcctcgtgcg gttccgcatc gtcccggaaa cgggacggct caccgcggtg atctctccca
     5821 ctgcccgccc ggcgcggtcc acgccccagc cggccccgct gggcggcgtc gagcgacgcc
     5881 gctcccaggg cgccgcggcg ccgccgaaag cgccgccccc gcccccgccc ccgccgcgcc
     5941 cgtcggctat cgcggcggcg ccgttgatcg agagcccggt cgcgccaccg gagccaccac
     6001 cgcgagcggc gctgccgggg gaattcgccg gcgaccagta ccgcgccgcc gaagcgcgcc
     6061 tcgtcgccct cttgaagcag ctggtcggga gcgagtcgtc ggacttgcac ctgcgcgtcg
     6121 gggagccgcc ggtgttccgc acccacggcc agatcgcgcg gcaaccgggt cctccgctca
     6181 cgagcgacga gctggcgctg atgctgctgg cgatcatgcc ggagcgcaat cgcggcgagt
     6241 ggaaggagaa cggcgactcg gatttcgcct atgagatccc cggtctcgcc cggttccgcg
     6301 tcaacgcggg gcgcgaccgg aaggggccga tcgcggtgtt ccgcgtgatc ccggccaagg
     6361 tcgtgacggt cgaggagctc ggcctcggca aggacctgac gcagctctgc ttcctcaaca
     6421 agggactcgt gctggtgtcg ggacccacgg gatccgggaa gtcgacgacg ctgtgcgcgc
     6481 tcatcgattt catcaatcgc gagcgcaccg atcacgtcgt gacgatcgaa gaccccatcg
     6541 aattcgtgca cgagaacaag cgctgcctga tcacacagcg acaggtgcac ctccacaccg
     6601 agagcttcaa gtcggcgctg cgcgccgcac tacgcgaaga tccggacatc gtgctggtgg
     6661 gcgagatgcg cgacctcgag accatcgcca tcgcgatcga gaccgccgag acgggacatc
     6721 tcgtgttcgg gacgctgcac accacgacgg cggcgtcgac cgtggaccgg atcatcgatc
     6781 agtttcccgc cgatcggcag gcgcagatcc gcgtcatgct gtccgagtcc ctgcgggggg
     6841 tgatctgtca gaccctgtgc cggaagatcg gcgggggacg cgtggcggcg cgggaggtcc
     6901 tgctgtcgac cccggcggtg tcgaacctga tccgcgaggg caagacctac cagattccgt
     6961 ccatcatcca gaccagccgc aaggccggga tgatcaccct caacgatgcc ctgctcgacc
     7021 tggtggaaaa gcgccagatc gagcccgacg aggcctacct ccgttccgtg gagaaggcgc
     7081 ccatgctgca ggcgctcagg gcgaagggcc acgccgttac ggtcggcgcg gccccgggag
     7141 agcgtccggc tacgggatga tgtcgaagga ctggctggtg atggtggtga ggccggagga
     7201 tgcggccgta atggtgtagc cgctcgcgac gacgttgata ctcaggtccg tgaacgtcga
     7261 cacgccggag acgaacgaaa cggtcgtggt cccggaaagc gacgtgacgc ccgggttgga
     7321 gcccagggcg agtgtcacgc ctccggtggc cgtggtgtcc cgcacaccgg tgatgctgtc
     7381 cctgacttcc acctgcacgg tgttgatgaa ggcggggaag acgtgtgacg taacgggctg
     7441 cgcgatgaac gcgagaatgc ggccgccgcc gccaccgccg ccgataccga cctggtgtgg
     7501 cgcggtcgcc gacgggcacc ccgccaagat cagcgcaccg attcccgtca cgaacagtcg
     7561 tgcgcgtcgc atgagctccc tccccaagag gtgattcgcc ggtttgacaa gccccgccgg
     7621 cgagccctac cttccggctt cacaattagt taggcgctca ggaggttgtc aatggcgctg
     7681 cgcacacaag cccccgaagg cgaccacccc gggagcgccc gcgccgcgga tcccatcccc
     7741 aactacatcg gcggcgcctg ggtcccctcc agggccaccc atgcgctgcc gctgatcaat
     7801 ccggccaccg gtgaaacgct ggcccaggtg ccgctcgcgg gaaaggcgga cgtggatgcg
     7861 gccgtccagg cggcgcagga cgcgttcccc cgctggcgcg ccactcctcc ggtggtgcgg
     7921 gcccgtcacc ttttcaccct gaaggggctg ctcgagaccc acttcgacga gctggccgcg
     7981 atcgtgaccc gggaaaacgg caagacgctc gacgaatccc ggggctcgat ccgccgtgcc
     8041 atcgagaatg tcgagcatgc ctgcggcatt cccacgctcc tgatgggcga cgcactcgag
     8101 gacgtggcga ccggcatcga ttgcgagtac gtgcgccagc cgatgggcgt cttcgcggcg
     8161 atcac
//
LOCUS       DBNGZW010000283         5211 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_427131, whole genome shotgun sequence.
ACCESSION   DBNGZW010000283 DBNGZW010000000
VERSION     DBNGZW010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5211)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_427131"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..347)
                     /locus_tag="AC16JC_06775"
     CDS             complement(<1..347)
                     /locus_tag="AC16JC_06775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR5164436.1"
                     /translation="MKRRAVGRSRTLWSQPAKGGADALMLAYTVGDDRHWDARLLPWD
                     VLGSLGHVEGLRRSGLISASDYRVLRAGLRAGLPAARRGTLRIGPDQEDAHSAVEQWL
                     TRRVGRAGERLHTG"
     gene            complement(344..1423)
                     /locus_tag="AC16JC_06780"
     CDS             complement(344..1423)
                     /locus_tag="AC16JC_06780"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5164437.1"
                     /translation="MKPTSPTEIALLTELVAIPSVSGDEAAVAQRVEESARAWGLDVV
                     RSATGVKLEVRGKRPGSTLALVSHLDVVPPGEGWTRDPFTPELEDGRLYGRGSGDAKA
                     SVAAMLSAARDVATQGGPPSGRLLVLLGYGEETRHTSMPELVASAGPIDAAVVGEPTN
                     LDIAVAQRGLMMVDLVARGDQRHAGYADENGGRFVNAAQRLARDLISLGNVARERPHP
                     VLGHPTITPTMLEAGVGRNVTPPLARAVLDVRTTPAWSHTEMAAALRAALESEVVVTS
                     DRLVPCETPRDSRLLAAARRVRPEARTFGSPTCSDWVFLRDRDAIKCGPGTSRNSHVP
                     DEWVTVAEVSAAREFYARLVREYLA"
     gene            complement(1401..2342)
                     /gene="argB"
                     /locus_tag="AC16JC_06785"
     CDS             complement(1401..2342)
                     /gene="argB"
                     /locus_tag="AC16JC_06785"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR5164438.1"
                     /translation="MTAAIEPAKGIVGLKGALRYVRAYRDQVFVVKLGGEVLDDADVL
                     DGVAAQLALLASLGIRIVVVHGGGAQATALSRRLGLEPELVAGRRITDQRTLEVVTMV
                     YGGTLNVAVLSALRGHRVQAVGISGVDGDLLTAHRRPPVTVTDDDGNTREVDYGFVGD
                     IDRVDPHLLKTLLDARFVPVVAGLAGDEEGGVYNVNADTVAESLAVATGAQKLIFLTG
                     APGVLRDRNDPSTLVTFADPSDLEELMASGALAGGMRPKVEACIRAATKGVERTHIID
                     GRAPDALLLEVFTGVGCGTMIVGRKERAEQLEADLAD"
     gene            complement(2339..3355)
                     /locus_tag="AC16JC_06790"
     CDS             complement(2339..3355)
                     /locus_tag="AC16JC_06790"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HIR5164439.1"
                     /translation="MAGRDFLALEDWGRPALDDLLTLAARIKRGEITGGLERKVLAMV
                     FLDPSLRTRSSMETAMFLHCGHALVLEPGKGSWALETEPGVVMDGATVEHVTEAARVL
                     ARYADALAVRAFPKGTDWAVEREDRLIKDFARFSDKPVINLESSRRHPCQALADAMTL
                     RERLGETRGKKFVLAWAWHPKALPTAVPASAALAAAQLGMEVVIARPPGFDLDPDDQA
                     LIDKLARESGGSARLCDDLDAGLAGADAVYVKSWGSLQAFGHPEAERALRTPFREWRM
                     TAPRLRVTRGGRGIVMHCLPVRRNVEIDDAVLDGPNSVVVDEAENRLHAQRALLIEIM
                     GGKR"
     gene            complement(3352..4530)
                     /locus_tag="AC16JC_06795"
     CDS             complement(3352..4530)
                     /locus_tag="AC16JC_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR5164440.1"
                     /translation="MLKTMPTGANAPEHATDALFPVYARMPVRPVSGRGSWLIDGDGH
                     EWLDAYGGHAVASTGHCHPHVVEAIARQAGKLLFYSTAVALEVRERLASELVARCQVP
                     GARVFLCNSGAEANENALHLARRATGRSGVVAVEGGWHGRTVATLACTHGEKYQAWAR
                     AAGMPLARFVPFDDVAALERTVDETTAALIVEPVQGVQGARDCAPEFLRAARAACDRH
                     GAALIFDEIQCGVGRCGAFTAAETYGVTPDVITMAKGLASGLPIGAILVGPKLATGVK
                     TGDLGSTFGGGPVVCAAALATLEVIDRERLIANAIAIGEQLRRSALALGVSKVQGRGL
                     LLGLHLGRPAAEVQKALFVRRILTGTSADSQVLRLMPPLTLSAGEADRLLEALAEVLR
                     "
     gene            complement(4524..>5211)
                     /locus_tag="AC16JC_06800"
     CDS             complement(4524..>5211)
                     /locus_tag="AC16JC_06800"
                     /inference="COORDINATES: protein motif:HMM:NF046896.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Asd/ArgC dimerization domain-containing protein"
                     /protein_id="HIR5164441.1"
                     /translation="PHAAPALRSRFVYGLADVCGESLHGARAIAVPGCFATAAALALY
                     PLTRTDLAAASPPALYAITGSSGAGVGPKPTTHHPARAHNVFAYGVLGHRHDAEITEQ
                     WRNWTGGGSRPAPRLVPHAGPFVRGIYLTLHARLEHGGDVAAAYDSTYAGRPFVRVRK
                     APPELTHVVGTNDAQLHATASEDGRELQVSVAIDNLAKGAAGQAVQAMNLALGLEETA
                     GLRFGGIYPC"
BASE COUNT          706 a         1944 c         1848 g          713 t
ORIGIN      
        1 ccggtatgga gccgctcccc ggcccggccc acgcgcctgg tcaaccactg ctcgaccgcg
       61 ctgtgcgcgt cctcctgatc cggcccgatc cgcagcgtgc cgcgccgcgc cgcgggcagt
      121 cccgcccgca atccggcgcg cagcacacgg tagtcggacg cggaaatcag ccccgagcgc
      181 cgcagccctt cgacgtgccc caggctcccc agcacgtccc aggggaggag acgggcgtcc
      241 cagtgacggt cgtcgcccac cgtgtaggcg agcatcagtg cgtccgcccc accctttgcg
      301 ggctgggacc acagcgtacg ggaccggccg acggcgcggc gcttcacgcc aggtactccc
      361 gcacgaggcg cgcgtagaac tcccgcgcgg cgctgacctc ggcaacggtc acccattcat
      421 cgggaacgtg gctgttgcgg ctcgtgccgg ggccgcactt gatcgcgtcc cggtcccgca
      481 ggaataccca gtcggagcag gtgggactgc cgaaggtgcg cgcctcgggc cgcacgcgcc
      541 gcgcggcggc gagcagccgc gagtcccgcg gggtctcgca cggcacgagc cggtcggacg
      601 tgaccacgac ctcgctctcc agcgcggcgc gcagcgctgc agccatctcg gtatgcgacc
      661 aggcgggcgt cgtgcgtacg tcgagcaccg cacgggcgag cggcggcgtc acgttgcgtc
      721 ccacgccggc ctcgagcatg gtcggcgtga tggttgggtg gccgagcacc ggatggggcc
      781 gctcgcgcgc cacgttcccg aggctgatca ggtcgcgcgc caggcgctgc gccgcgttga
      841 cgaaccgccc gccgttttcg tcggcataac cggcgtggcg ctgatcgccg cgtgccacga
      901 ggtccaccat catcaggccg cgctgcgcca cggcgatatc gaggttcgtg ggctcgccca
      961 cgacggccgc gtcgatgggg cctgcggacg ccaccagctc cggcatcgag gtgtgacggg
     1021 tttcctcacc gtagcccagc agcacgagga ggcgcccact cggcgggccg ccctgggtgg
     1081 ccacgtcgcg tgcggcggag agcatggccg ctaccgaggc cttggcatcc cccgagccgc
     1141 gcccgtagag cctgccgtcc tccagttcgg gcgtgaaggg atcgcgcgtc cacccttcgc
     1201 cgggcggcac gacgtcgagg tgcgagacca gggcaagggt cgagccgggg cgcttgcccc
     1261 gcacttcgag cttcacgccg gtcgcgctcc gcacgacgtc gagcccccag gcgcgcgcgg
     1321 actcctcgac gcgctgcgcc acggccgcct cgtcgccact caccgacgga atggcgacga
     1381 gctcggtcag gagcgcgatc tcagtcggcg aggtcggctt caagctgctc cgccctttcc
     1441 ttgcggccca cgatcatggt cccgcacccc acgccggtga atacctcgag cagcagcgcg
     1501 tcgggcgcgc gtccgtcgat gatatgggtg cgctcgacgc ccttggtcgc ggcgcggatg
     1561 caggcctcga ccttggggcg catcccgccc gccagcgccc cgctcgccat cagctcctcc
     1621 agatcgctcg gatcggcgaa cgtcacgagg gtcgaggggt cgttccggtc gcgcagcacg
     1681 ccgggggcgc cggtgaggaa gatcagcttc tgcgcgccgg tcgcgaccgc gagggattcg
     1741 gcgacggtgt cggcattgac gttgtagacg ccgccctctt cgtcgcccgc gaggccggcc
     1801 acgaccggga cgaaccgggc gtcgagcagc gtcttcagga gatgcgggtc cacccggtcg
     1861 atgtcgccca cgaatccgta gtccacctcg cgcgtattcc cgtcgtcgtc ggtcacggtg
     1921 accggtgggc ggcggtgcgc ggtgaggaga tcgccgtcca caccggagat gcccaccgcc
     1981 tgcacgcgat ggccccgcag cgctgacagc acggcgacgt tgagcgtgcc gccgtacacc
     2041 atcgtgacca cctcgagggt gcgctggtcg gtgatgcgcc gcccggccac gagctcgggc
     2101 tccagcccga gccggcgcga caacgcggtc gcctgggccc cgccgccgtg caccaccacg
     2161 atgcggatgc cgagcgacgc cagcagcgcg agctgggccg ccacgccgtc caacacgtcg
     2221 gcgtcgtcca gcacctcgcc gccgagcttg accacgaaca cctggtcgcg gtaggcccgc
     2281 acgtagcgca gcgcgccctt caaacccacg atcccctttg ccggctcgat cgccgccgtc
     2341 atcgcttgcc tcccatgatc tcgatcagca acgcccgctg ggcgtggagg cggttctccg
     2401 cctcgtccac caccaccgaa ttgggcccgt cgagcaccgc gtcgtcgatc tcgacgttgc
     2461 ggcgcaccgg caggcagtgc atcacgatgc cgcggccgcc gcgcgtgacg cggagccggg
     2521 gcgccgtcat ccgccactcg cggaacggcg tgcgcagggc gcgctccgct tccgggtggc
     2581 cgaacgcctg cagcgacccc cagctcttca cgtagacggc gtccgcgccc gcgaggccgg
     2641 cgtccaggtc gtcgcacagc cgcgccgagc cgcccgattc gcgtgccagc ttgtcgatga
     2701 gcgcctggtc gtcggggtcg aggtcgaagc ccggcggccg ggcgatgacg acctccatac
     2761 cgagctgcgc ggcggcgagg gcggcgctcg ccggcacggc cgtgggcaat gccttgggat
     2821 gccacgccca ggccagcacg aacttcttgc cgcgcgtctc ccccagccgc tcgcgcagcg
     2881 tcatcgcatc ggccagcgcc tggcaggggt gacggcgcga ggactcgagg ttgatcaccg
     2941 gcttgtccga gaagcgcgcg aagtccttga tcaggcgatc ctcccgctcc accgcccagt
     3001 cggtgccctt ggggaaggcc cggaccgcga gggcgtcggc gtagcgcgcc agcacgcgcg
     3061 ccgcctcggt gacgtgctcc accgtggcgc cgtccatgac cacgcccggc tcggtctcga
     3121 gcgcccagct gcccttgccc ggctccagca cgagcgcgtg gccgcagtgg aggaacatgg
     3181 cggtctccat gctgctgcgc gtgcgcaggc tcgggtcgag aaagaccatc gccaggactt
     3241 tgcgctcgag cccgcccgtg atctcgccgc gcttgatgcg ggcggcgagg gtgaggagat
     3301 cgtcgagcgc cgggcgaccc cagtcctcga gcgcgaggaa atcgcggccg gctatcgcag
     3361 cacctccgcg agcgcttcca gcagtcggtc ggcttccccg gccgagaggg tgagcggggg
     3421 catgagccgc agcacctgcg agtcggcgga ggtcccggtg aggatccgcc gcacgaatag
     3481 ggccttctgc acctctgccg cgggacggcc gaggtgcagg ccgagcagca gtccccggcc
     3541 ctggaccttc gagacaccga gcgccagcgc gctgcgccgc agctgctccc cgatcgcgat
     3601 cgcgttggcg atcagcctct cgcggtcgat cacttcgagc gtggcgagcg ccgcggcgca
     3661 caccacgggg ccgccgccga aggtgctgcc caggtcgccc gtcttcaccc ccgtggctag
     3721 cttcgggccg accaggatgg cgccgatcgg gagccccgag gcgagtccct tggccatcgt
     3781 gatgacgtcc ggggtgacgc cataggtctc ggcggcggtg aaggcgccgc accggcccac
     3841 gccgcactgg atctcgtcga agatcagcgc cgcgccgtgg cgatcgcagg cggcgcgcgc
     3901 cgcgcgcagg aattccggcg cgcagtcccg cgccccctgc acgccctgca cgggctcgac
     3961 gatcagcgcc gcggtcgtct cgtccaccgt gcgctcgagc gccgcgacgt cgtcgaacgg
     4021 cacgaagcgc gcgagcggca tcccggcggc ccgcgcccag gcctggtact tctcgccgtg
     4081 ggtgcaggcg agcgtcgcca cggtgcggcc gtgccagccg ccttcgaccg cgacgacgcc
     4141 cgagcggccg gtcgcgcggc gtgccaggtg cagcgcgttc tcgttggctt cagcaccgga
     4201 attgcacagg aagaccctgg cacccggcac ctgacacctg gccaccagct ccgatgccag
     4261 ccgttcgcgc acctccaacg ccaccgccgt cgaatagaac agcagcttcc ccgcctggcg
     4321 cgcgatcgcc tcgacgacgt gggggtgaca gtggccggtc gacgccaccg cgtgcccgcc
     4381 gtaggcgtcg agccattcgt ggccgtcgcc gtcgatcagc caggagccac ggccgctcac
     4441 cggacgcacc ggcatccggg cgtagacggg gaacagcgcg tccgttgcgt gctccggtgc
     4501 gttggcgccg gtcggcatgg tcttcagcac ggatagatcc cgccgaaacg cagcccggcg
     4561 gtctcctcga gtcccagcgc aaggttcatc gcctgtacgg cctgtccggc cgctcccttc
     4621 gcgaggttgt cgatcgccac gctcacctgc agctcgcgcc cgtcttcgct cgccgtcgcg
     4681 tgcagttgcg cgtcgttggt gcctacgacg tgggtcagct cgggcggcgc cttgcggacg
     4741 cgcacgaacg gccgtcccgc ataggtggaa tcgtacgcgg cggccacgtc gccgccgtgc
     4801 tccagccgcg cgtgcagggt gaggtagatg ccgcgcacga acggcccggc gtgcgggacc
     4861 agacgcgggg ccggacggct cccgcccccc gtccaattgc gccactgctc ggtgatttcg
     4921 gcgtcgtggc ggtgacccag cacgccgtag gcgaacacgt tgtgggcgcg tgcgggatgg
     4981 tgtgtcgtgg gcttgggacc gaccccggcg cccgagctgc cggtgatggc gtacagggcc
     5041 gggggggagg cggccgcaag gtcggtgcgg gtgagcgggt acagcgcgag cgctgccgcg
     5101 gtggcgaagc acccgggcac cgcaatggcg cgggcgccgt gcagcgactc cccgcacacg
     5161 tcggccagtc cgtagacgaa gcgcgagcgc agggcaggcg ccgcatgggg t
//
LOCUS       DBNGZW010000284         7841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1594650, whole genome shotgun sequence.
ACCESSION   DBNGZW010000284 DBNGZW010000000
VERSION     DBNGZW010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7841
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1594650"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1094
                     /locus_tag="AC16JC_06805"
     CDS             24..1094
                     /locus_tag="AC16JC_06805"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR5164442.1"
                     /translation="MTGRNGLSCLVVDDEPRLRRVIAHLLEGDGFRVREAGTGVEALR
                     EMELDPAPLVISDLRMPEMDGITLLPHLLKRWPDTAVVMVSAVADVDTAVKCLKLGAL
                     DYVSKPFNLGEVRARVDQALQRRQLRLELQGYRAELEARVHAQERRIKELFLDRVEAL
                     AQALEEKDAYLRGHSLRVAEYAVQTARRLGFDEGTIDTVRLGARLHDLGKIGVSEEVL
                     HKPAKLTPEEYRHVMEHTVIGARVLGPLLRDHPGAIAIVRSHHERVDGKGLPDGLRGD
                     AIPPIVRIVSVADAFDAMTSERTYRHSLPVEEALDELRRQRGLQWDPTAVDGFLDAFP
                     DVHHLPIPTPDLSAGRAPQPVG"
     gene            1128..2552
                     /gene="glmM"
                     /locus_tag="AC16JC_06810"
     CDS             1128..2552
                     /gene="glmM"
                     /locus_tag="AC16JC_06810"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR03990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR5164443.1"
                     /translation="MPDTLMVGVSGIRGIVGKDLTPELVARYAAAFGAWARTGGARRP
                     RIVLGRDARTSGPMFMQAAAAGLMSVGCDVIDIGLTTTPSAQLAVEHHRAAGGIILTA
                     SHNPIEWNALKFVGPDGVFIDETAGAQVRALVAEGPIPRADHGNLGTVITDGEALKRH
                     LAAVVRLAGVDAARVRKRHFRVALDTCRGAGGAIMPALLERLGCEVAGINLETDGRFP
                     RPPEPIPEQLGALGAVVQRVQADIGLAVDPDVDRLAIVDEQGEPIGEDYTLAFAVRSV
                     LGGTRDGGRGKSKGAAGDTTPESRVVVCNLSTSLVVEDAARDCGALVVRTPVGEVHVA
                     RAMIERGAPIGGEGNGGVMYPALHVGRDAPVGAALILTLLARDGRRVSELVAASPRYA
                     IVKDKVPRSRTSGLTQVNAALRARFADATVDERDGLRLGWRDRWLHVRPSNTEPIIRL
                     IAEAPTAAEARQLVDVGRNLCAES"
     gene            2537..4369
                     /gene="glmS"
                     /locus_tag="AC16JC_06815"
     CDS             2537..4369
                     /gene="glmS"
                     /locus_tag="AC16JC_06815"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR5164444.1"
                     /translation="MCGIVGYTGRKQAAAIVLEGLKRLEYRGYDSAGIAVVGDGRLEV
                     VKAAGKISALEQALGSRLPAGRTALAHTRWATHGAPTDVNAHPHSDCGGTLAVVHNGI
                     IENATALRQALTGRGHVFRSETDTEVLAHLIEDVHGPGTSLGDAVVTALQQVQGTYGI
                     GVVSTREPDTIVAARRGSPLLVAIGSGEHFVASDAAAVLSYTRSVVYLDDGEVAIVRP
                     DDYRILDLAAVEKVKAVTRVEWDVATIERGGHAHFMLKEILEQPESLRNTLRGRLLEE
                     EGDVHLGGLNVSDEELQRINRIVFTGCGTSWHAGLIGEYMMEELARLPTEVEYASEFR
                     YRNPIVDDRTLVVGISQSGETADTLAALREAKRRGARTLGLVNVVGSTIAREVDGGIY
                     LHAGPEIGVASTKAFTSQIAALALLTLKMGRLRTLSILQGREVVRALARLPELVERTL
                     AKAREVEQQAERMIRATNVIYLGRGYNFPVALEGALKLKEISYIHAEGYPAAEMKHGP
                     IALIDDLMPVVFIAPRDAVYQKVLSNVEEVKARGGRVIAVVTDGDTGLSGLAEHAFSI
                     PETLDLLTPVLSVLPLQLLAYYIAVRRGCNVDQPRNLAKSVTVE"
     gene            4449..4919
                     /gene="dtd"
                     /locus_tag="AC16JC_06820"
     CDS             4449..4919
                     /gene="dtd"
                     /locus_tag="AC16JC_06820"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018705740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HIR5164445.1"
                     /translation="MGAGGQVRVVLQRVSRAEVRVEGRRIGGIGAGFVVLAGFAPTDT
                     DETLAWMAEKILSLRLFADAAGKMNRGLEELGGGGVLVVSQFTLYGDASKGRRPSFIA
                     AASPEVAIPLYERFVALLRERAARLGVAVATGEFGAMMDVDLVNDGPVTLILER"
     gene            4939..5889
                     /locus_tag="AC16JC_06825"
     CDS             4939..5889
                     /locus_tag="AC16JC_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008199192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5164446.1"
                     /translation="MNVAAALVLLIVALVIVVLLRWDRTFDAPLPAVRARTDSAVIAQ
                     GRYLVYGPAHCAYCHNTVETVAALDRGEQPPLAGGFVFRLPVGTFRAPNLTPDSATGI
                     GRLSDGQIARVLRFGVLPSGRAAVPFMAAQDLSDADLVAIVSFLRSQPPVRRAVPPHD
                     ITLLGKAVLAFVIKPIGPNGTPPVESPPPGPTVERGAYLVRVVAECTGCHTERSHIDG
                     SYTGPLLAGGTPIDAEGAPGLVAVPPNLTPDPGTGRIAAWSEDQFVARFRKGKLIPQS
                     PMPWGAFARMSDDDLRAIYRFLRTVTPVEHETGPSLRRSE"
     gene            5895..6485
                     /locus_tag="AC16JC_06830"
     CDS             5895..6485
                     /locus_tag="AC16JC_06830"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR5164447.1"
                     /translation="MQSPDSPIVLASASPRRRQLLEMLRIPFEVEPSGIDEVVHPGEA
                     PRDYVLRVAREKAGAVGARRPGALVLAADTKVVLDGEVFGKPADAAAAVGMLLRLQGR
                     THQVLTGVTVGRGGRLEQALDVTAVTFRPAEEAALSDYVATGEPLDKAGAYAVQGQGG
                     ALIARIEGDVFGVMGLPLRLVIDLLAQFGRPYRFTR"
     gene            complement(6467..>7841)
                     /locus_tag="AC16JC_06835"
     CDS             complement(6467..>7841)
                     /locus_tag="AC16JC_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR5164448.1"
                     /translation="VAFKVAGITPPARPSTPTPEASQTGGSRAMPQGTIVRQLPVSPS
                     SGGVKAIALETGPAAVGASHLKVKAESETERREKKLELLLEISKELSRQQELDQLLHK
                     VVDVTFQIMNVDRVSILLLDPRTKELVPKVSRSRTGDTNAAKLVPQSIARKAVEDRVA
                     ILSDNAAADDRFKGKSILIQSVRSAMCTPLLGSDQQALGIIYVDNQTAIHSFTDEDLE
                     FLIAFGNIVTTAVENSQLSERIRREAMVLSNFQRYFAPNVAAQIAQQEGAVTLGGDKR
                     PVVIFFSDIRGFTPMSENMNPDEVAHLLTEYFTEMVEIVFEHSGSLDKFMGDAIMALW
                     GAPIAHEDDADRAMQCAIDQLNALEKMNAKWKEQGRPPIKIGVGINFGEVFAGNFGSD
                     RRLEYTVIGDAVNTASRLCSVAGANEILIAEPFYKALKNPPAVEALEPMQVKGKTRPV
                     PVYRVKR"
BASE COUNT         1135 a         2617 c         2882 g         1207 t
ORIGIN      
        1 agcgaccctt acgttagccc gtgatgacgg gccgaaacgg gctttcctgc ctcgtggtgg
       61 atgacgaacc gcggctgcga cgcgtgatcg cccacctgct cgagggcgac gggttccgcg
      121 tgcgcgaagc cggcaccggg gtcgaggcgt tgcgggagat ggagctggac cccgcgcccc
      181 tcgtgatctc ggacctccgc atgccggaaa tggacggcat caccctgttg ccccacctcc
      241 tcaagcgctg gcccgacacc gcggtcgtga tggtgagcgc cgtcgccgat gtggacaccg
      301 ccgtcaaatg cctcaagctc ggcgcgctcg actacgtcag caagccgttc aacctggggg
      361 aggtacgcgc ccgggtggac caggcgctcc agcgccgcca gctgcggttg gagctgcagg
      421 gttaccgggc ggagctcgag gcacgcgtgc acgcgcagga gcggcgcatc aaggagctgt
      481 tcctcgaccg cgtcgaagcg ctcgcccagg cgctggagga gaaggacgcc tacctgcgcg
      541 gccactcgct gcgcgtcgcg gagtacgcgg tgcagacggc tcggcgactc gggttcgacg
      601 aggggacgat cgacaccgtc cggctgggtg cgcggctcca cgacctcgga aagatcggcg
      661 tgagcgagga ggtgctgcac aagccggcga agctcacgcc cgaggaatac cgccacgtca
      721 tggagcacac ggtgatcggc gcgcgcgtcc tcggaccact gctgcgcgac caccccggcg
      781 ccatcgcgat cgtgcggtcg catcacgagc gcgtggacgg caaggggttg ccggacggcc
      841 tgcggggcga cgcgatcccg ccgatcgtcc ggatcgtctc ggtggcggat gcgttcgatg
      901 cgatgacgag cgagcggacc taccggcatt cgctgccggt cgaggaggcg ctggacgagc
      961 tgcgtcggca acggggcctc cagtgggatc ccacggcggt cgacgggttc ctggacgcct
     1021 tccccgacgt tcaccacctc cccatcccga cgcccgacct ctccgccgga cgcgctcctc
     1081 agccagtcgg ttgacgcggc gttccgtcct cccgtaacgt tccggccatg cccgacaccc
     1141 tgatggtcgg cgtctccgga atccgcggca tcgtcggcaa ggatttgacg cccgagctcg
     1201 ttgcccgcta cgccgccgcc ttcggcgcgt gggcgcgcac ggggggcgcg cgccgtcccc
     1261 ggatcgtgct gggccgcgac gcgcggacct cgggccccat gttcatgcag gcggcggcgg
     1321 cgggactcat gtccgtcggc tgcgatgtga tcgacatcgg actcaccacc acgccctccg
     1381 cgcagctcgc ggtggagcat catcgcgccg cggggggcat catcctcacc gcgagtcaca
     1441 acccgatcga atggaacgcc ctcaagttcg tgggccccga cggcgtcttc atcgacgaga
     1501 ccgcgggggc gcaggtgcgt gccctggtcg ccgagggacc gattccccgc gccgaccacg
     1561 gcaacctcgg caccgtcatc accgacgggg aggcgttgaa gcggcatctg gccgcggtgg
     1621 tgcggctggc gggggtggac gccgcgcggg tgcgcaagcg ccacttccgg gtcgcgctcg
     1681 acacctgccg cggggcgggc ggcgccatca tgccggccct gctcgagcgg ctcggctgcg
     1741 aggtcgccgg catcaatctg gagaccgacg gccggttccc gcggcctccc gagcccattc
     1801 cggagcagct cggagcgctg ggggccgtgg tgcagcgggt gcaggcggac atcggcctgg
     1861 cggtggaccc cgacgtggac cgcctcgcca tcgtggacga acagggggag ccgatcgggg
     1921 aggactacac gctggcgttc gcggtccgct cggtgttggg agggacgagg gacgggggac
     1981 gggggaagag caaaggcgcc gccggtgaca cgacgcccga gtcgcgtgtc gtggtgtgca
     2041 acctctcgac cagtctggtc gtagaagacg cggcgcgcga ttgcggtgcc ctggtggttc
     2101 ggacgccggt gggggaagtc cacgtggcgc gggcgatgat cgagcgtggt gcgccgatcg
     2161 ggggagaggg caacggcggc gtgatgtacc cggcgctcca cgtgggacgc gacgcgccgg
     2221 tcggagccgc gttgatcctc acgctcctgg cgcgggacgg gcgccgcgtg agcgagctgg
     2281 tggccgcctc gccgcggtat gcgatcgtga aggacaaggt accgcggagc cggacgtcgg
     2341 gcctgaccca ggtcaatgct gccctgcgag cgcggtttgc ggatgccacg gtggacgagc
     2401 gcgacggcct gcggctgggc tggcgcgacc gctggctgca cgtgcggccc tcgaacaccg
     2461 agcccatcat ccggttgatc gcggaagcgc cgacggcggc ggaagcgcgg cagctggtgg
     2521 acgtgggacg aaacctatgt gcggaatcgt agggtacacc ggccggaagc aggccgccgc
     2581 catcgtcctg gaaggactga agcggctcga gtatcgcggg tacgactcgg ccggcatcgc
     2641 cgtcgtgggc gacggccgcc tcgaggtggt caaggcggcc gggaagattt ccgcgctgga
     2701 gcaggcgctg ggcagccggc tccccgccgg gcgcaccgcc ctggcgcaca cgcgctgggc
     2761 cacccacggg gcgcccaccg acgtgaacgc gcacccgcac tcggactgcg gcggcaccct
     2821 cgcggtcgtc cacaacggca tcatcgagaa cgccaccgcg ctgcggcaag cgctcacggg
     2881 gcggggccac gtatttcgct ccgagacgga caccgaggtg ctggcccacc tgatcgaaga
     2941 cgtgcacggg ccggggacct ccctgggcga tgccgtggtg accgcattgc agcaggtgca
     3001 gggcacctac gggatcgggg tggtgtcgac ccgggagccc gacacgatcg tcgcggcccg
     3061 gcgtggctcg ccgctgctcg tggccatcgg ctcgggcgag cacttcgtgg cttccgatgc
     3121 cgcagccgtg ctgtcgtaca cgcggtcggt cgtgtatctg gacgacggtg aggtcgccat
     3181 cgtgcggccc gacgactacc gcatcctcga tctcgccgcc gtcgagaagg tcaaggccgt
     3241 cacgcgggtg gagtgggacg tggccaccat cgagcgcggc ggacacgcgc acttcatgct
     3301 gaaggagatc ctcgagcagc ccgagagcct gcgcaatacc ctgcgcgggc ggctgctcga
     3361 agaggaaggc gacgtgcacc tcggggggct gaacgtctcc gacgaggagc tgcagcgcat
     3421 caaccgcatc gtgttcacgg gttgcgggac gtcgtggcac gccgggctga tcggcgaata
     3481 catgatggag gagctggcgc ggctgcccac cgaggtcgag tacgcctcgg agttccgcta
     3541 tcgcaatccg atcgtggacg accgcacgct ggtggtcggg atctcgcaat cgggcgagac
     3601 ggcggacacg ctggcggcgt tgcgtgaggc gaagcggcgc ggggcgcgca ccctgggtct
     3661 cgtcaacgtg gtgggcagta cgatcgcgcg ggaggtcgac gggggcatct acctgcacgc
     3721 cggtcccgag atcggcgtcg cctcgaccaa ggcgttcacc agccagatcg ccgcgctggc
     3781 gctgctcacg ctcaagatgg ggcggctgcg gacgctctcg attctccagg ggcgcgaggt
     3841 ggtccgcgcg ctggcgcggt tgccggagct ggtcgagcgc accctcgcca aggcgcggga
     3901 ggtcgagcag caggccgagc gcatgatccg cgcgacgaac gtgatctacc tgggccgcgg
     3961 ctacaacttc ccggtggcgc tcgagggggc gctgaagctc aaggagatct cctacatcca
     4021 cgccgagggg tatcccgccg ccgagatgaa gcacgggccg atcgccctga tcgacgacct
     4081 gatgccggtg gtgttcatcg cgccacgcga cgcggtctat cagaaggtgc tctcgaacgt
     4141 ggaagaggtg aaggcgcgcg gggggcgcgt gatcgcggtc gtcacggacg gcgatacggg
     4201 tctgtcgggc ctggccgaac acgcgttctc gatccccgaa accctcgacc tgctcacgcc
     4261 ggtgctctcg gtcctgccat tgcagctgct cgcctactac atcgccgtgc gccgcggctg
     4321 caacgtggac cagccgcgca acctcgcgaa gagcgtgacg gtcgagtgaa gcaaatgcgg
     4381 aaggcggagt gcggaatgcg gaatcgacga ccgccgttcc attccgcatt ccgcattcca
     4441 cattccgcat gggggcgggg ggccaggtga gggtagtgtt gcagcgggtc agccgcgccg
     4501 aagtgcgcgt ggagggccgg aggatcgggg ggatcggggc gggattcgtg gtgcttgcgg
     4561 ggtttgctcc caccgatacc gacgagacgc tcgcctggat ggccgagaag atcctgtcgc
     4621 tgcggctctt cgccgacgcg gcgggcaaga tgaatcgggg tctggaggag ctgggcggcg
     4681 gcggcgtcct ggtcgtttcc caattcacgc tgtacggcga cgcctccaag ggacggcggc
     4741 ccagcttcat cgcggccgcg tctcccgagg tggccatccc gctctacgag cgcttcgtgg
     4801 cgctgctccg cgagcgcgcc gcacggctcg gcgtcgcggt cgccaccggc gaattcggcg
     4861 ccatgatgga cgtggacctg gtgaacgacg ggccggtgac cctgatcctg gagcgttgac
     4921 atactgcgca agatcctgtt gaacgtcgcg gcggccctgg tgctcctcat cgtcgctctg
     4981 gtcatcgtgg tcctgctgcg ctgggatcgc accttcgacg cgccgctgcc ggccgtccgg
     5041 gcccgcaccg attcggcggt gatcgcccag gggcggtacc tggtctacgg gccggcgcac
     5101 tgcgcctatt gccacaacac ggtggaaacc gtcgccgccc tggaccgtgg cgagcagccg
     5161 ccgctggccg gcggcttcgt cttccgtctg cccgtcggga cgttccgcgc tccgaatctc
     5221 acgcccgatt ccgcgacggg aatcggccgc ctgagcgacg ggcagatcgc cagggtactt
     5281 cgcttcggcg tgctgcccag cggccgcgcc gctgtgccgt tcatggccgc gcaggacctg
     5341 agcgacgcgg acctcgtcgc catcgtgtcg ttcctccgct cgcagccgcc ggtgcggcgc
     5401 gccgtccctc cccacgacat caccctcctc ggcaaagccg tcctggcatt cgtgatcaaa
     5461 ccgatcggcc cgaatggcac gccgccggtg gagagtccgc cgcccggacc cacggtggag
     5521 cgtggggcgt acctggtgcg ggtcgtggcg gaatgcacgg gctgccacac ggagcggagc
     5581 cacatcgacg gctcgtatac cggaccgctg ctcgccggcg gaacgccgat cgatgcggag
     5641 ggggcgccgg ggctcgtggc ggtgccgccc aatctcacgc ccgatcccgg gaccggccgc
     5701 atcgccgcgt ggagcgagga ccagttcgtc gcacggttcc gaaaggggaa gctgatcccc
     5761 cagtcgccca tgccgtgggg cgcgttcgca cggatgagcg acgacgacct gcgggcgatc
     5821 taccgcttcc tgcgcaccgt cacccccgtc gagcacgaaa ccggaccgtc actgcggcgc
     5881 agcgagtgac cgaggtgcag tcgccggatt ccccgatcgt cctcgcgagc gcgagtccgc
     5941 ggcggcgcca gctgctcgag atgttgcgca tcccgttcga ggtggagccc tcggggatcg
     6001 acgaggtcgt gcacccgggc gaggcgccgc gcgactacgt cctgcgggtg gcgcgggaga
     6061 aggctggggc cgtcggggcc cggcggcccg gcgcgctcgt cctcgcggcg gacaccaaag
     6121 tcgtgctgga tggcgaggtc ttcggcaagc cggcggatgc ggctgcggcg gtggggatgc
     6181 tgctgcggct ccagggccgg acccatcagg tcctcaccgg ggtgacggtc ggccggggcg
     6241 gccggttgga gcaggcgctc gacgtcaccg ccgtcacctt ccgccccgcg gaggaggcgg
     6301 cgctgtccga ctacgtcgcc acgggtgagc cgctcgacaa ggcgggggcg tacgcggtgc
     6361 aggggcaggg cggcgccctg atcgcgcgca tcgaggggga cgtcttcggg gtgatgggac
     6421 tgccgctgcg gctggtgatc gacctgctgg cgcagttcgg gcggccctac cgtttcacgc
     6481 ggtagaccgg caccggcctc gtcttgccct ttacctgcat cggctccagc gcctcgacgg
     6541 ccggcgggtt cttcagcgcc ttgtagaacg gctcggcgat gaggatctcg ttggccccgg
     6601 cgaccgagca gagccggctg gcggtgttga cggcgtcgcc gatcacggtg tactcgaggc
     6661 gccggtctga gccgaagttg ccggcgaaca cttcgccgaa gttgatgccg acgccgatct
     6721 tgatcggcgg ccggccctgt tccttccact tggcgttcat cttctccagg gcgttgagct
     6781 ggtcgatggc gcactgcatg gcgcggtcgg cgtcgtcctc gtgggcgatc ggcgcgcccc
     6841 acagcgccat gatggcgtcg cccatgaact tgtcgagcga gcccgagtgc tcgaacacga
     6901 tctccaccat ttcggtgaag tactcggtca ggaggtgcgc cacctcgtcc ggattcatgt
     6961 tctccgacat cggcgtgaag ccgcggatgt cggagaagaa gatcaccacc ggccgcttgt
     7021 ccccgcccag ggtgacggcg ccttcctgct gggcgatctg ggcggctacg ttgggcgcga
     7081 aatagcgctg gaagttcgac agcaccatcg cttcgcggcg gatgcgctcg gagagctggg
     7141 agttctccac ggccgtggtg acgatgttgc cgaaggcgat caggaactcc aggtcctcgt
     7201 cggtgaacga gtggattgcc gtctggttgt cgacgtagat gatgccgagg gcctgctggt
     7261 cggacccgag cagcggcgtg cacatcgcgc tgcgcacgct ctggatgagg atcgacttgc
     7321 ccttgaagcg gtcgtcggcg gcggcgttgt cggagagaat cgcgacgcgg tcctccacgg
     7381 ctttccgcgc gatcgactgc gggacgagct tggcggcgtt ggtgtcgccg gtgcggctgc
     7441 gggagacctt ggggaccagc tccttggtcc gggggtcgag caacaggatc gacacccggt
     7501 ccacgttcat gatctggaac gtgacatcca ccaccttgtg cagcagctga tccagctcct
     7561 gctgacgcga cagctccttg gagatctcga gcagcagctc gagcttcttc tcgcgccgct
     7621 cggtctcgga ctcggctttc accttgagat gcgacgcgcc caccgccgcg gggccggttt
     7681 cgagggcgat cgccttcacc ccacccgacg agggactgac gggcagctgc cgcacgatcg
     7741 ttccctgcgg catggcgcgc gagccgccgg tctggctcgc ctcgggcgtc ggggtcgagg
     7801 gacgggcggg tggcgtgatg cccgcgacct tgaaggccac c
//
LOCUS       DBNGZW010000285         3048 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1381528, whole genome shotgun sequence.
ACCESSION   DBNGZW010000285 DBNGZW010000000
VERSION     DBNGZW010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1381528"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1723)
                     /locus_tag="AC16JC_06840"
     CDS             complement(<1..1723)
                     /locus_tag="AC16JC_06840"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HIR5164449.1"
                     /translation="MRPHRLLPALLLSAPALSAQGFDFYSRGPYRAGVPRPEAITGYR
                     AGEQHTMYAVMQRYLDTLLATAPDRSRAETWGYTTEHRPYRALIISDSANLARLDQIR
                     GAITELTDPRRTTLARANAIAAAEPIVVLFQYSVHGDEPAGFEAALQVAYQMLAGEDS
                     VTRTILKNVVLVLNPAANPDGHERFAAWYNSVAVGADNPWAFEQNEPWSITGRYNHYR
                     FDMNRDLVAQSQPEARIMMDGILRWHPQVFVDHHSTTQTFFFPPVAPAVNQNLPAQAP
                     HWFEVFGRGNAAAFDRYGWQYFVRGVFDFFYVGYWDEWTTFQGATGMTYETDGGPEFN
                     KRRDDGTITTFRDGIAHHFIASLATLETAARNRQARLQDYYAFRKSAMDEAAGDRMKR
                     IVLVPGTDARMAAHVAGLLLRNGIEVTRLTQPAAAAQAHSYMGGPAARRTFPAGSYVV
                     ELAQPQRRLAKALLEPNAVLERPFVQDQVDRFRRNRRRGEDADKDEYGFYDVTAWSLP
                     YTYNVEAYWTEDAAPLGGQPVTDTVLPAPAAPGAARSAYVFPNDNLGAARLALALEQE
                     GFNVAVAL"
     gene            1917..2462
                     /locus_tag="AC16JC_06845"
     CDS             1917..2462
                     /locus_tag="AC16JC_06845"
                     /inference="COORDINATES: protein motif:HMM:NF015513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR5164450.1"
                     /translation="MRHGFDWNGFAFGFGPRGFAMGMGPRHRRRRQWFESGDMKYVIL
                     KLLREKPRHGYEVMKELEEQLHGCYSPSPGTVYPTLQWLEDEGLVRARDQEGKKVYEI
                     TDAGITFLDEHKDVVTDIFDRVAETVEEALGGSMAEINQGVARVMKAVYRRGWRGGDE
                     ARHRIAEILARAAKEVEEVLP"
     gene            2459..2902
                     /locus_tag="AC16JC_06850"
     CDS             2459..2902
                     /locus_tag="AC16JC_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020510040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1707 SHOCT-like domain-containing protein"
                     /protein_id="HIR5164451.1"
                     /translation="MTTVSIRASDAEREQVTGILQHAVAEGRLTPEEGGERLAAASAA
                     RFRDELGRLVRDLPAIVETERPAPRRAPWIWMAARLVRGVVFGVLFAALLFWGIGLFL
                     PFWIFGFLVLAIVMGGRARRYRAMRWGGWGWYGRPGRFRPSSLPW"
BASE COUNT          470 a          974 c         1117 g          487 t
ORIGIN      
        1 ggagcgccac cgccacgttg aagccttcct gctcgagcgc gagcgcgagt cgcgcggcac
       61 cgaggttgtc gttgggaaac acgtaggcgg accgggcggc gccgggcgcc gcgggcgccg
      121 ggagcacggt atcggtcacc ggttggccgc ccaggggggc ggcgtcctcg gtccagtacg
      181 cctcgacgtt gtacgtgtag ggcagcgacc aggcggtgac gtcgtagaag ccgtactcgt
      241 ccttgtcggc gtcctcgccg cggcgacgat tgcggcggaa ccggtcgacc tgatcctgga
      301 cgaacggccg ctccagcacg gcgttgggct cgagcagcgc cttggcgagc cggcgctgcg
      361 gctgcgcgag ctccaccacg tagctccccg cggggaaggt gcgtcgggcg gccgggccgc
      421 ccatgtagga atgggcctgc gccgccgcgg ccggctgcgt gaggcgcgtc acctcgatcc
      481 cgttgcgcaa cagcagcccg gcgacgtgcg ccgccatgcg ggcgtccgtg ccgggcacca
      541 ggacgatccg tttcatgcga tcgcctgccg cctcgtccat cgccgacttg cggaatgcgt
      601 agtagtcctg cagccgcgcc tgccggttgc gcgccgccgt ctcgagcgtg gccagcgacg
      661 cgatgaagtg atgggcgatg ccgtcgcgaa acgtggtgat cgtgccgtcg tcgcgccgct
      721 tgttgaattc cgggccgcca tcggtctcgt aggtcatgcc ggtggcgccc tggaacgtcg
      781 tccactcgtc ccagtagccg acgtagaaga agtcgaacac gccgcgcacg aagtactgcc
      841 agccgtagcg gtcgaacgcc gccgcatttc cccggccgaa cacctcgaac cagtgcgggg
      901 cctgcgccgg gagattctga ttcaccgccg gcgccaccgg gggaaagaag aacgtctgcg
      961 tggtggagtg atgatccacg aacacctgcg gatgccagcg caggatgccg tccatcatga
     1021 tgcgcgcctc gggctggctc tgcgccacca ggtcgcggtt catgtcgaag cggtagtggt
     1081 tgtagcgccc cgtgatgctc cagggctcgt tctgctcgaa cgcccacggg ttgtcggcgc
     1141 cgacggccac cgagttgtac caggcggcga accgctcgtg cccgtccggg ttggcggccg
     1201 ggttgagcac cagcacgacg ttcttcagga tcgtgcgcgt caccgagtcc tcccccgcca
     1261 gcatctggta ggcgacctgc agcgccgcct cgaatcccgc cggctcgtcg ccgtgtaccg
     1321 agtactggaa cagcacgacg atcggctccg ccgcggcgat cgcgttggcg cgcgccaggg
     1381 tcgtcctgcg ggggtcggtg agctccgtga tcgcgccgcg gatctgatcc aggcgcgcca
     1441 ggttggcgga atcggaaatg atcagcgccc gatacgggcg gtgctccgtc gtgtagcccc
     1501 aggtctcggc gcgggagcgg tcgggagccg tggcgagcag cgtgtcgaga taccgctgca
     1561 tcacggcgta catcgtgtgc tgctcgcctg cccggtagcc cgtgatcgcc tccggtcgcg
     1621 gcaccccggc gcggtagggg ccgcgggagt agaagtcgaa gccctgggcg gaaagcgccg
     1681 gagcagaaag caggagagcg gggagcagtc gatgcggtcg catgggccgt cgcgcgtaag
     1741 ggtggctgga ttgggcggca gaatgtatcc ctggcggcgt atcgcgcaac cgcaagcatc
     1801 tgatggggtt ggaggtgcgg cttgacagtg gctgaaacac gatatagcgt ggcgacgtat
     1861 cttacgtaac atcgtgacgt agcatctcaa gacataacgc agaacaggag cgcaccatga
     1921 gacacggatt cgactggaac ggcttcgcct ttgggttcgg gccccggggc ttcgccatgg
     1981 gcatgggccc acgccaccgc cgtcgccgcc aatggttcga gtcgggcgac atgaagtacg
     2041 tcatcctcaa gctgctgcgg gaaaagccgc gccacggcta cgaagtgatg aaagagctcg
     2101 aagagcagct gcacggctgc tactccccct cgcccggcac cgtctatccc accctgcaat
     2161 ggctggaaga cgaagggctg gtgcgcgccc gtgaccagga aggcaagaag gtctacgaga
     2221 tcaccgacgc cgggatcacg ttcctggacg agcacaagga cgtcgtgacc gacatcttcg
     2281 accgcgtggc ggagacggtc gaggaggcgc tgggtggctc gatggcggag atcaatcagg
     2341 gggtggcccg cgtcatgaag gcggtgtatc ggcgcggctg gcgcggcggc gacgaggcgc
     2401 gccaccgcat cgccgagatt ctcgcgcggg ccgcaaagga ggtcgaggag gtactcccat
     2461 gaccacggta tcgatccgcg cttccgacgc ggaacgcgag caggtgaccg gcatccttca
     2521 gcatgccgtg gcggaaggac ggctgactcc cgaggaaggg ggcgagcgcc tcgcggcggc
     2581 gagcgccgcc cgcttccgcg acgagttggg acggctggtt cgtgacctgc cggcgatcgt
     2641 cgagacggag cgacccgccc cgcgccgtgc gccgtggatc tggatggcgg cgcggctggt
     2701 ccggggcgtc gtcttcggcg tcctgttcgc cgcgctcctg ttctggggca tcggactctt
     2761 cctgccgttc tggatcttcg ggttcctggt actggccatc gtgatgggtg gtcgggcgcg
     2821 gcgctatcgc gcgatgcgtt ggggcggctg gggctggtac ggcaggccgg ggcgattcag
     2881 gccgtcgagc ctgccgtggt agcgccgccc gcgatcgaaa cgcgggcatt gcgcaaagtg
     2941 tatcgcaccc cgccgcccgg catggtgccg cgcggcaagg cggcggggcc tgtggagatc
     3001 gtcgcattgg acgcgctcga tctccaggtg ggcgcagggg aattcttc
//
LOCUS       DBNGZW010000286         7918 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_700507, whole genome shotgun sequence.
ACCESSION   DBNGZW010000286 DBNGZW010000000
VERSION     DBNGZW010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7918)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7918)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7918
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_700507"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1891
                     /gene="hypF"
                     /locus_tag="AC16JC_06855"
     CDS             <1..1891
                     /gene="hypF"
                     /locus_tag="AC16JC_06855"
                     /EC_number="6.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013076453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016743 - carboxyl- or
                     carbamoyltransferase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HIR5164452.1"
                     /translation="FQYPYINCTDCGPRYSIILGLPYDRAATTMSAWAMDARCAAEYH
                     DPASRRFHAQPVACPECGPHYRLRGGVGGDTDAEVIAAAVARLRGGDILAVKGLGGYH
                     LACDARNRAAVAALRERKFRKEKPFALMARDLEVARRLVELSPAAQTLLTSPARPIVL
                     APARAELPGVAPDNRDLGVMLPYTPLHYLLFAAGAPEALVMTSANRSNEAIAYEDADA
                     FERLSGIADAFLVGERPIARRVEDSVVCDGAFGPATLRRGRGLAPSAVTVLPTTRPIL
                     ALGADLKNALTLVVDGQAFVSQHIGDLDDYSALEAFERTIADLLEMYRVDPALLVVAH
                     DAHPQYRSTTQAERLPAGARMSVQHHRAHVASVLAERAAWERQVLGVSFDGTGYGDDG
                     SIWGGEIFAGSLDEGFERVAHLRPAALVGGDAAARHPVQAAAGYLHQVEALPDLAAEP
                     FGFPARYGDVVRLLESGTRVFPTTSTGRLFDAAAALLGFTRAVTFEGQAAIWLEHLAR
                     SAGDVEPYPFPFSDGELDFRPLLHAVAQDRQRGRAAAEIARAFQAGVAHGVREACVVL
                     CAARRIGTVVVSGGVFQNDLLLRDLQPLLERESLDVWINHAVPANDGGISLGQAALAA
                     LRYPS"
     gene            1888..2736
                     /gene="era"
                     /locus_tag="AC16JC_06860"
     CDS             1888..2736
                     /gene="era"
                     /locus_tag="AC16JC_06860"
                     /inference="COORDINATES: protein motif:HMM:NF000908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HIR5164453.1"
                     /translation="MTRCGTVVLAGLPNAGKSSLLNALVKERLAIVSPKAQSTRAPVV
                     GLLTRGADQFIFTDPPGLLEPKYRLQEKMKEAALRTVADAEVIAYLHPLPEYPAPPFE
                     RVAGLDRSPAAPVVTVYTKCDLVPPAKRPGGEALVVSAVSGEGLEALLDRLAERLPPG
                     PFHYQPDELATQPLRFFAAEYVREAAFELLEEELPYSIACEIDEFRENERPAYIRAVV
                     YVERESQKGIVIGDKGRTIKAIGRAARERIESLMGGPVYLDLHVKVLENWRRKDPILR
                     RLGYDS"
     gene            2733..2903
                     /locus_tag="AC16JC_06865"
     CDS             2733..2903
                     /locus_tag="AC16JC_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR5164454.1"
                     /translation="MSLAAELLEILVCPKCKGPLEYRTGPAEELVCHTCRLIYAVEDD
                     IPIMLIDEAKSF"
     gene            2903..3898
                     /locus_tag="AC16JC_06870"
     CDS             2903..3898
                     /locus_tag="AC16JC_06870"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR5164455.1"
                     /translation="MRRSRGALACLILCAYPAVGLSAQNQPINAAALFLLLPISARSV
                     GMGQTAAALDGHGEDVFLNPAGVGSMTSSEFALHSARLAAGPATALTVFFPRRGIGVF
                     GAAVYLVDYGDQPIVDDQNVTLGRIGARNLAFLTTYATQLSDAVSLGVSYKLIEFRVD
                     CAGASGLCPSLSGEGVTHALDLGGQFSVGPDHALRVGVALRNLGFALQVNNEAQADPL
                     PTRLAFGAAYTVQLRPMGDGPASNRFDLQVAADVESPWRETGTPDVRVGMDLGYQQVV
                     RVRGGYAYRHEGLSGPSIGLGVATGVIGVDLAQMFLTHTDLIVSNPTFLSFRVLF"
     gene            3916..4692
                     /locus_tag="AC16JC_06875"
     CDS             3916..4692
                     /locus_tag="AC16JC_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164456.1"
                     /translation="MAVLVFLYATRAAGQDPDSTVVRARARLWLPPVASLIVPGSGQL
                     VKGRDRGMVYLAAEAFLLTRFLQLRHDGRRGAQRYRDLAFAVARRGFASARRDTTFEY
                     YEQMERYTASGEFNRNPDPNGTLIPEDDLTTYNGSRWLIAQQTYFPDPNNPPPPNSQP
                     YLDALTFYRNEAVGPDFRWSWRDASLEQQEYRATIRSSDDAFRSAQNQLGLLLANHLV
                     SAIDALISTRLSEGLRREASVETTWMGRLPGSRIQVTIAF"
     gene            4727..5449
                     /gene="rph"
                     /locus_tag="AC16JC_06880"
     CDS             4727..5449
                     /gene="rph"
                     /locus_tag="AC16JC_06880"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012181215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0009022 - tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HIR5164457.1"
                     /translation="MSPTSPPRPDGRRSTALRPLTLERRANPNAEGSCLVRLGGTVVL
                     CTASVEAGVPAWRKGSGLGWVTAEYAMLPRATQDRTPRERQGAGGRTHEIQRLIGRSL
                     RAALTGFAFGEHTIRIDCDVLQADGGTRTAAITGAAVALSDACDWLVARAGVANPFRG
                     LVAAVSVGIVAGEPRLDLCYAEDRDAEVDANVVVAEPDRLIEVQATAERGTFDRAGLD
                     GLLDLAREGTLRIFAAQHAALT"
     gene            5446..6495
                     /gene="cax"
                     /locus_tag="AC16JC_06885"
     CDS             5446..6495
                     /gene="cax"
                     /locus_tag="AC16JC_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014901755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015369 - calcium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006816 - calcium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/proton exchanger"
                     /protein_id="HIR5164458.1"
                     /translation="MTWLRWLLLALPVSWALAAFHGPALAVFVVSGLGVIPLAAIMGE
                     ATEALARRTGPTVGGLLNATFGNAAELILAIVALRVGLIDLVKASLTGSILGNLLLIL
                     GLSLVAGGAKRPSLTFNRTAAGMSGAMLLLAVVGLVMPALFASTHPAATQATTIHLSE
                     GVAVVLGGTYVLGLVFSLVTHRQLFGAGAPEHGGPDAAPRVGRAGAMLAGATLLVAVQ
                     SEILVHAVEQVVMHLGWSEFFLGLMVIPLVGNAAEHASAVVAARRGHTDLALTIALGS
                     STQVALFVAPLLVLAGVLVGQPMQLVFTGFEVVALGLASLIVTVIALDGESHWFEGAQ
                     LLAVYAILAIAAWFV"
     gene            6553..7545
                     /locus_tag="AC16JC_06890"
     CDS             6553..7545
                     /locus_tag="AC16JC_06890"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing response regulator"
                     /protein_id="HIR5164459.1"
                     /translation="MRTRPTILFFSATGRSAPEFVARWMQGSGKTLVPVHDPAELEQM
                     VLRALPSLVLVDADATGARGLELCRRLKMDPFTAIAPLVAVSGRHTTERVMEWFSAGA
                     DEVITPLLEPSEQSGRLDALLTRTARDVAVNPSTRLPGTTEIERDVRRRMENGERFAV
                     CYADLDHFKEYNDRYSYYDGDRVIYLVSRILHDVVKGMVERRGFVGHIGGDDFIFIIP
                     VPDIAEVCTEVLSVFDTLIPFQYNEQDRRAGYYFGKDRRGQLHRVPLMTLSIGIVTNE
                     HRRFAHPAQVSELATEMKSYAKTQPGSVFVVDRRHDSEPGALEPNARSSARSSQ"
     gene            7542..>7918
                     /locus_tag="AC16JC_06895"
     CDS             7542..>7918
                     /locus_tag="AC16JC_06895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HIR5164460.1"
                     /translation="MNVICTSCETVYRVDPAKVPASGVRARCAVCGNVFAVQPDGAGA
                     RSGSPLAPAPAAAPATRISVPIGPVFTPAPAPPPPTPPATFSPAAPPAPPAPPAPAVQ
                     ATPPPPSPVRPSTGPFARPPFVAP"
BASE COUNT         1099 a         2742 c         2761 g         1316 t
ORIGIN      
        1 cttccagtac ccgtacatca actgcaccga ttgtggcccg cgctactcca tcatcctcgg
       61 cctcccctac gatcgcgccg ccaccacgat gagcgcctgg gcgatggatg cccgctgcgc
      121 cgccgaatat cacgatccgg cgagccgtcg ctttcacgcc cagccggtgg cctgccctga
      181 atgcgggcca cactaccgtc tccgcggtgg ggtgggagga gacaccgatg ccgaagtgat
      241 cgccgcggcc gtcgcccggc tgcgcggcgg cgacatcctc gccgtgaaag ggctcggcgg
      301 ctaccacctg gcctgcgatg cgcgcaaccg cgccgcggtc gcggcgctgc gcgagcgcaa
      361 gttccgcaag gagaagccgt tcgccctgat ggcgcgcgac ctcgaggtcg cgcgccggct
      421 ggtcgagctg tcacccgccg cccagacgct gctcacgtcg cccgcacggc ccatcgtgct
      481 cgctcccgcc cgcgccgagc tgccgggcgt cgctcccgac aatcgcgatt tgggtgtcat
      541 gctcccctac accccgctgc actacctgct gttcgccgcc ggcgcgccgg aggcgctcgt
      601 catgacgagt gccaaccgct cgaacgaagc cattgcctac gaggacgccg acgccttcga
      661 gcggctctcg gggatcgcgg acgcgttcct cgtgggcgag cgacccatcg cgcggcgcgt
      721 cgaggattcc gtcgtgtgtg acggcgcctt cgggcctgcg acgctgcgtc gcggtcgagg
      781 gctggcgccc agcgcggtca cggtcctgcc cacgacccgc ccgatcctgg cgctgggggc
      841 cgatctcaag aacgccctca ccctggtcgt cgacggtcag gcgttcgtca gccagcacat
      901 cggggatctg gacgactact cggcgctcga agcgttcgag cgcaccatcg ccgatctgct
      961 cgagatgtat cgggtggacc cggcgctgct cgtggtggcg cacgacgccc atccccagta
     1021 ccgctcgacc acgcaggccg agcggctccc ggccggcgcg cgaatgtcgg tccagcacca
     1081 tcgggcgcac gttgcctccg tgctcgccga gcgcgccgcc tgggaacggc aggtactcgg
     1141 cgtgagcttc gacggcaccg ggtacggcga cgacgggtcg atctggggtg gggagatctt
     1201 cgcggggagt ctcgacgaag gcttcgagcg cgtcgcgcac ctgcgcccgg cggccctggt
     1261 cggtggcgac gcggccgccc ggcatcccgt gcaggcggcc gccgggtatc tccatcaggt
     1321 ggaggcgctg ccggatctcg ctgccgagcc gttcgggttt ccggcccgct acggcgatgt
     1381 ggtgcgactg ctggaatccg gcacgcgcgt atttcccacg acgtcgacgg ggcggctgtt
     1441 cgatgccgcc gccgccctgc tgggcttcac gcgcgccgtg acgttcgagg gccaggcggc
     1501 gatctggctc gagcacctcg cccggtcggc gggggatgtc gagccgtatc cgtttccgtt
     1561 cagcgatggt gagctcgact tccggcccct gctgcacgcg gtcgcgcagg accggcagcg
     1621 cggccgggcg gccgccgaga tcgcgcgcgc gtttcaggcg ggtgtggcgc atggggtacg
     1681 cgaggcatgc gtggtgctgt gcgctgcccg acgcatcggc acggtcgtag tgtcgggggg
     1741 cgtgttccag aacgacctgc tgctgcgcga tctccagccg ctgctcgagc gggaatcact
     1801 cgacgtgtgg atcaaccatg cggtgccggc caatgacggc gggatcagtc tggggcaggc
     1861 ggcgctcgcc gcgctgcggt acccgtcatg acgcgctgcg gcacggtggt cctggccggc
     1921 ctgcccaacg ccggcaagtc ctcgttgctc aacgcgctgg tcaaggagcg cctggcgatc
     1981 gtgtcgccca aggcgcagtc tacccgggcg cccgtcgtgg ggctgctgac gcgcggagcg
     2041 gaccagttca tcttcaccga tcccccggga ctgctcgagc cgaagtaccg gctgcaggag
     2101 aagatgaaag aagcggctct gcggacggta gcggacgcgg aggtcattgc gtacctgcac
     2161 ccgctgccgg aatatccagc gccgcccttc gaacgcgtcg cggggctcga ccggtcgccg
     2221 gccgccccgg tcgtcacggt gtataccaag tgcgatctcg tgccgccggc gaaacggccg
     2281 ggcggtgagg cattggtcgt gtcggcggtg tccggggaag ggctcgaggc gctgctcgac
     2341 cggctggccg agcgcctgcc gccggggcct tttcactatc agcccgacga gctggcgacg
     2401 cagccgctgc ggtttttcgc cgccgaatat gtccgggaag cggcgttcga gctgctcgag
     2461 gaagagctgc cctacagcat cgcctgcgag atcgacgagt tccgggagaa tgagcgcccg
     2521 gcgtatattc gggccgtggt gtacgtggag cgggagagcc agaagggaat cgtcatcggg
     2581 gacaagggac gtactattaa ggcgatcggt cgcgccgcgc gcgagcgcat cgagtccttg
     2641 atgggaggac cggtctacct ggacctgcac gtgaaggtgc tggagaactg gcgtcgcaag
     2701 gacccgattc tccggcgcct gggatacgac tcatgagcct cgccgccgag ctgctcgaga
     2761 tcctcgtctg ccccaagtgc aaggggccgc tggagtaccg caccggtccg gccgaggagt
     2821 tggtgtgtca cacctgccgg ctgatctacg cggtggaaga cgacattccc atcatgctga
     2881 tcgacgaggc gaagagcttc tgatgcgtcg gtcccggggc gccttggcgt gtctgattct
     2941 gtgcgcatac ccggccgtcg gtctgtctgc ccagaatcag ccgatcaacg cggccgcgct
     3001 gttcctgctc ctgcccattt ccgcccggtc ggtcgggatg gggcaaaccg ccgcggcact
     3061 cgatgggcac ggggaggacg tcttcctgaa tccggcgggc gtcggctcga tgacctcgag
     3121 cgagttcgcg ctgcatagtg cacggctggc cgccggtccc gcgacggcgc tgacggtgtt
     3181 cttcccccgg cgcggcatcg gcgtgttcgg cgcggccgtg tatctggtcg actacggcga
     3241 ccagcccatc gttgacgacc agaacgtcac cctgggccgc atcggtgccc ggaacctggc
     3301 gttcctgacg acgtatgcga cccagctcag cgatgccgtt tcgctcggcg tgagctacaa
     3361 gctgatcgaa ttccgggtgg actgcgcagg agcaagcgga ctgtgtccgt ccctgtcagg
     3421 ggagggcgtg acgcacgccc tcgacctcgg cggccagttc agcgtcgggc ccgatcatgc
     3481 gctgcgggtc ggcgtggcgc tgcgcaacct gggattcgcg ctccaggtga acaacgaggc
     3541 ccaggccgac ccgctgccga cgcggctggc cttcggcgcc gcgtataccg tccagctgcg
     3601 ccccatgggc gacggccccg ccagtaaccg cttcgatctg caggtggccg cggacgtcga
     3661 aagcccatgg cgcgaaacgg gcaccccgga cgtccgcgtc ggcatggacc tgggctatca
     3721 gcaggtcgtg cgggtgcgcg gcgggtatgc gtaccggcat gaaggactct cgggccccag
     3781 catcggtctg ggcgtggcga cgggggtgat cggtgtggac ctggcgcaaa tgtttctcac
     3841 acacaccgat ctgatcgtct ccaacccgac ctttctctcc ttccgggtgt tgttctgata
     3901 cgcgcgccct ggccggtggc cgttctggtg ttcctgtacg cgacccgcgc ggccgggcag
     3961 gacccggact ccacggtcgt ccgcgcccgc gcgcggctat ggttgccgcc cgtcgcctcg
     4021 ctgatcgtgc cgggctcggg tcagctggtg aaggggcggg accgggggat ggtgtacctg
     4081 gccgcggagg cgttcctcct cacgcgattc ctccagctca ggcacgacgg acgccgcggc
     4141 gcccagcggt atcgcgacct cgccttcgcg gtcgcgcgcc gtgggttcgc gtcggcccgg
     4201 cgcgacacca cgttcgagta ctacgaacag atggagcgct acacggcgag cggggaattc
     4261 aaccggaacc cggaccccaa cggcacgctg atcccggagg acgatctcac gacgtacaac
     4321 ggttcccggt ggctcatcgc acagcagact tatttcccgg atccgaacaa cccgcctccg
     4381 cccaactccc agccctacct cgacgccttg accttctacc gcaacgaggc ggtcggaccg
     4441 gatttccgct ggtcctggcg cgacgcctcg ctcgagcagc aggaataccg ggcgacgatc
     4501 cgctccagcg acgacgcctt tcgcagcgcc cagaaccagc tcggcctgct gctggccaac
     4561 cacctcgtca gcgcgatcga cgcattgatt tccacgcggc tctccgaggg cctccggcgg
     4621 gaggcgagcg tggagacgac gtggatgggg cgcctgccgg ggtcgcgcat tcaagtgacg
     4681 atcgccttct agcgcgcagc acgatccacc cacatcctca tctcccatgt cgccgacatc
     4741 accgcctcgc ccggacggac gtcgttcgac cgccctccgt ccgctcaccc tcgagcggcg
     4801 cgcgaatccg aacgcggagg ggtcctgtct cgtccgtctc ggcgggaccg tcgtgctgtg
     4861 cacggcgtcg gtcgaggccg gcgtccccgc gtggcgcaag ggatccggcc tggggtgggt
     4921 cacggccgaa tacgccatgc tgccgcgcgc gacccaggat cgcaccccac gggagcggca
     4981 gggggcgggc gggcgcacgc acgagattca gcggctgatc ggccggagcc tgcgcgccgc
     5041 gctcaccggc ttcgccttcg gcgagcatac catccggatc gactgcgacg tgctccaggc
     5101 cgacggcggc acgcggaccg ccgcgatcac cggggcagcc gtcgcgctgt cggacgcctg
     5161 cgattggctc gtcgcgaggg ccggcgtggc gaacccgttc cgcgggctcg tggcggccgt
     5221 gagcgtcggg atcgtcgccg gtgagccgcg cctcgacctg tgttatgccg aagaccggga
     5281 cgccgaggtg gacgccaacg tcgtggttgc cgagcccgat cgcctgatcg aggtccaggc
     5341 cacggcggag cggggcacgt tcgaccgcgc cggactggac gggctcctcg atctggcgcg
     5401 ggagggcacg ttgcgcatct tcgcggcgca acacgcggcg ctcacgtgac ctggctgcgc
     5461 tggttgctgc tggcgctccc ggtttcgtgg gctctggcgg cgttccacgg accggcactc
     5521 gcggtgttcg tcgtctcggg actgggagtg attccgctcg cggccatcat gggtgaggcg
     5581 accgaggcgc tcgcccgtcg caccggtccc acggtcggtg ggttgctcaa cgccaccttc
     5641 ggcaatgccg ccgagctcat cctggcgatc gtggcgttgc gggtggggct catcgacctc
     5701 gtcaaggcct cgctgaccgg gtcgattctg ggcaacctgc tgctcattct cggcctgtca
     5761 ctcgtagccg gcggcgcgaa acgtccatcc ctcaccttca atcggaccgc cgcgggcatg
     5821 agcggtgcga tgctgctgct ggcggtggtg ggcctggtca tgccggcgct gttcgcctcg
     5881 acacatccgg cggcgaccca ggcgacgacc attcacctct ccgagggcgt cgcggtcgtg
     5941 ctgggcggga cgtacgtgct gggcctggtg ttctccctgg tcacgcaccg ccagctgttc
     6001 ggggcggggg cgccggagca cggcgggccg gatgccgcgc cccgtgtggg ccgggcgggc
     6061 gcgatgttgg cgggagcgac gctactcgtc gccgtgcaga gcgaaatcct cgtccacgcc
     6121 gtggagcagg tggtgatgca cctcggctgg tccgagttct tcctggggct gatggtcata
     6181 ccgctcgtcg ggaacgccgc cgagcatgcg tccgccgtgg tcgcggcgcg ccggggacac
     6241 acggatctgg ccctcacgat tgcgctcggt tccagcacgc aggtcgcgct tttcgtggcc
     6301 cctctcctcg tgctcgccgg cgtgctggtc gggcaaccga tgcagctcgt gttcacgggg
     6361 ttcgaggtcg tcgccctggg cctggcctcc ctgatcgtga ccgtgattgc gctcgacggg
     6421 gaatcccact ggttcgaggg ggcgcagctg ctcgccgtat acgccattct ggccatcgcg
     6481 gcgtggttcg tttgacacgc gaggccgagt ggcgtaagtt taggcgcact cgacccttac
     6541 gcgaggtgcc ggttgcggac gcgccccacg attctcttct tttccgcgac tggccgttcc
     6601 gctccggagt tcgtggcgcg ctggatgcag ggcagcggga agacgctggt cccggttcac
     6661 gaccccgccg agctcgagca gatggtgctt cgcgccttgc cctccctggt gctcgtggac
     6721 gcggatgcga ccggcgcgcg ggggttggag ttgtgccggc ggctcaagat ggacccgttt
     6781 acggccattg cgccgctcgt ggcggtgagc gggcggcaca caaccgagcg ggtcatggag
     6841 tggttctccg ccggggcgga cgaggtgatc acgccgttgc tcgagccgag cgagcagtcg
     6901 ggccggctcg acgcgctgct cacccgcacg gcgcgggacg tcgccgtgaa tccctcgacc
     6961 cggttgcctg ggacgacgga aatcgagcgc gacgttcggc gccgcatgga gaatggcgaa
     7021 cggttcgccg tgtgctacgc cgatctcgat cacttcaagg aatacaacga ccgctacagc
     7081 tactacgacg gggatcgcgt catctacctc gtgtcacgga tcctgcacga cgtggtcaag
     7141 ggcatggtgg agcggcgggg gttcgtgggc cacatcgggg gagacgactt catcttcatc
     7201 atcccggtcc ccgacatcgc ggaggtctgc accgaagtgc tctcggtatt cgacacactg
     7261 atcccgtttc aatacaatga gcaggaccgg cgagcgggtt actacttcgg taaggaccga
     7321 cgcgggcaac tgcaccgcgt gccgttgatg acgttgtcga tcgggatcgt gaccaacgag
     7381 caccgccgct tcgctcatcc ggcgcaagtc agcgagctgg caacggaaat gaagagctac
     7441 gccaagaccc agcccgggtc ggtgttcgtg gtcgatcgtc gccatgattc cgagccggga
     7501 gccctggagc cgaacgcgag atcgagcgcg aggtcgtcgc agtgaacgtc atctgcacgt
     7561 cgtgcgaaac cgtttaccgt gtggatccgg ccaaggtccc ggcctccggc gtgcgtgcgc
     7621 gatgcgccgt gtgcggcaac gtcttcgccg tccagcccga tggcgccggc gccaggagcg
     7681 gcagccccct ggctccggct cccgcggctg ctcccgccac gcggatcagc gtcccgatcg
     7741 ggcccgtctt cactccggcc ccggcgcctc cgccacccac gccgcccgcg acgttctcgc
     7801 ctgctgcgcc accagcacca ccagcgccgc ccgcaccggc cgtgcaggca acgccgcccc
     7861 cgccgagccc tgtgcggccc agcaccgggc cgttcgcccg tccgccgttc gtcgcgcc
//
LOCUS       DBNGZW010000287         2660 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_912082, whole genome shotgun sequence.
ACCESSION   DBNGZW010000287 DBNGZW010000000
VERSION     DBNGZW010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2660)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2660)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2660
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_912082"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..1378)
                     /locus_tag="AC16JC_06900"
     CDS             complement(107..1378)
                     /locus_tag="AC16JC_06900"
                     /inference="COORDINATES: protein motif:HMM:NF025684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HIR5164461.1"
                     /translation="MTVHLAITLALLAQSGAAPTRARRPETPPVRVWLPDSTPSVGDP
                     AQVYVALRDTSHLVVLHVDPSGRVRVLFPRSPRESDLVPGGATFAVGGIEDGLTFRVP
                     GPGIGTVLAVRASARTPFRFDGLTTGEGWDYAHALLLQPTAGNPFAALLDIADRIANG
                     EAYNYDLTGYRTPGATAARRLQTDTVCFSCLAARHSGGGGSAEGYGEISNSAIAIDHS
                     YAAAPGGTVVDCSSATLVDSFCGVQDNSVSTTVTETSTYESTSYVTPIEEPFFFPFHR
                     FRRMRREPAPAPFPPAIALNLRRVPGRVVPPPARKPPRIILQQPTARSRPIAAPVAEA
                     PAAPAAVTGSRYIVRRPAPATPRADGSAAATAGGWTAAAPRETGTSATPRVAAPAPAP
                     AAAVARPAAQAARPMATPSRTGTRAYANPRD"
     gene            1440..>2660
                     /locus_tag="AC16JC_06905"
     CDS             1440..>2660
                     /locus_tag="AC16JC_06905"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR5164462.1"
                     /translation="MTRENYEALVQRLEGYARAHPDRYAVRVGLLAGLGYAYVWLVLV
                     LAAGLLAWLVRIEIRAEFRGAGIIRITVALALFVYTILRALWVRFEAPAGIVVGRHDV
                     PELMTLVDELVTAVQAPVFDQVVVSDDFNAAVSQQPRFGLVGGYRSYLIVGLPLAYGL
                     TAEEFRAVLAHEVGHLSRAHGRFGAWIYRVRVMWVRLTGQLQAQRTWGAWLFEWFLKR
                     WSPYFNAYSFVLARAQEYEADRFAARVGSAEALGRALARLEVAGSWIAQRFWGGVRRQ
                     LAHADEPPIGVIEEYSAAMRGAIPPDQSTRWLKQALARRTDATDTHPSLAERLRAMGV
                     ASDVATAATVAPPRAGSSAAERYFGESADRLATLLDAQWRIVVGPAWRAGHEAAARKT
                     SRLEELERRAAGGPL"
BASE COUNT          367 a          859 c         1037 g          397 t
ORIGIN      
        1 aagggggaag aggccccgca cccgtcccgc ttctcctttc tcttttcctc accctctggt
       61 tggcttgatg gatcgaccgg cagcggacgg agtgcgagca gcgcgctcag tcgcgcgggt
      121 tggcgtaggc cctggtgcct gtgcgcgagg gagtcgccat cggccgtgcg gcctgagcgg
      181 cgggtctggc aacggcggcg gctggggccg gagccggagc cgcgacgcgg ggtgtggcgc
      241 tggtgccggt ctcgcgcggc gctgccgcgg tccagccgcc ggccgtggcg gcggcagacc
      301 cgtccgcgcg cggtgtggcg ggagcggggc ggcggacgat gtagcgtgag ccggtcaccg
      361 ctgcgggagc cgccggcgcc tccgcaacgg gtgcggcgat cggccggctc cgggcggtgg
      421 gctgctgcaa gatgatgcgg ggaggcttgc gtgcaggggg cggcacgacc cgtcccggca
      481 cgcgacgcag gttcagggcg atcgccgggg ggaacggcgc aggggcgggc tcgcgccgca
      541 tgcgccggaa gcggtgaaac gggaagaaga acggttcctc gatcggcgtg acgtacgacg
      601 tcgactcgta ggtcgaggtc tcggtcaccg tcgtcgacac actgttgtcc tggacgccgc
      661 agaacgaatc caccagggtg gcgctcgagc aatccaccac ggtgcctccc ggcgccgcgg
      721 cgtagctgtg atcgatggcg atggcggagt tgctgatctc gccgtacccc tccgccgagc
      781 cgcccccgcc ggagtgccga gcggcgaggc agctgaagca caccgtatcc gtctggagcc
      841 gccgggccgc cgtcgcgccg ggcgtgcgat aaccggtgag atcgtagttg taggcttcgc
      901 cgttcgcgat ccggtcggca atgtccagca acgccgcgaa cgggttcccc gccgtcggct
      961 gcaacagcag ggcgtgcgca tagtcccagc cttcgcccgt cgtgagtccg tcgaaccgga
     1021 agggggtccg cgcgctcgcc cgcacggcga gcacggtccc gatcccgggc cccggaaccc
     1081 ggaacgtcag gccatcctcg atccccccga cggcgaaggt tgctccgccc ggcacgagat
     1141 cggactctct cgggctgcgc gggaacagca cgcggacgcg gcccgacggg tcgacgtgca
     1201 agacgacgag gtgactggtg tcgcgcagcg cgacgtagac ctgcgccggg tcgcccacgg
     1261 acggggtgga gtcggggagc cagacgcgaa cggggggcgt ctcggggcgg cgggcgcggg
     1321 tgggcgcggc cccggactgg gccagaagcg cgagtgtgat tgccaggtgg accgtcatga
     1381 gctctcgtcg tactgtaccc catccggtag aaaacggtca agggcaacgg gagccggaca
     1441 tgacgcgcga gaactacgag gcgctggtgc aacgtttgga agggtacgca cgggcccatc
     1501 cggatcgcta tgccgtgcgt gtcggtctgc tggccggcct cggctacgcg tacgtctggc
     1561 tggtgctggt gctcgccgca gggctactcg cgtggctcgt ccgaatcgag atccgggccg
     1621 aattccgcgg cgccggaatt attagaatca ccgtggcgct ggcgctgttc gtgtacacca
     1681 tcctgcgggc gctgtgggta cgattcgagg cgccggccgg gatcgtcgtc gggcgccacg
     1741 acgtcccgga gctgatgacc ctggtggacg agctggtcac ggcggtccag gccccggtgt
     1801 tcgatcaggt cgtggtgagc gacgacttca acgcggccgt ttcacagcag ccgcgcttcg
     1861 gcctggtggg cggataccgc agttacctga tcgtcgggct gccgctggcg tacgggttga
     1921 cggccgagga attccgcgcg gtgctggcgc acgaagtggg acatctctcc cgcgcccacg
     1981 gccggttcgg cgcctggatc taccgggtgc gggtgatgtg ggttcgcctg accgggcagc
     2041 ttcaggcgca gcgcacctgg ggcgcgtggc tcttcgagtg gttcctgaag cgctggagtc
     2101 cctacttcaa cgcctattcg ttcgtgctgg cccgggcgca ggaatacgag gccgaccggt
     2161 tcgcggcacg cgtggggagt gccgaagccc tgggtcgggc gctggcccgg ctcgaggtgg
     2221 ccggcagctg gatcgcgcag cggttttggg gaggcgtgcg ccggcagctc gcccatgccg
     2281 acgagccgcc gatcggcgtg atcgaggagt actcggcggc gatgcgagga gcgattccgc
     2341 cggatcagtc gacccgctgg ctcaagcagg cgctcgcccg gcggaccgat gcgaccgata
     2401 cacacccaag cctcgccgag cgattgcggg cgatgggtgt cgcgtcggac gtcgcgacgg
     2461 ctgcgacggt ggctccgcca cgggcaggct cgtccgcggc ggagcggtac ttcggcgaga
     2521 gcgccgaccg cctggcgact ctgctcgatg cgcagtggcg catcgtggtg ggccccgcat
     2581 ggcgggcggg gcacgaagcg gcggcccgca agaccagccg gctggaggag ctcgagcggc
     2641 gggcagcggg tggaccgctc
//
LOCUS       DBNGZW010000288         4331 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1790711, whole genome shotgun sequence.
ACCESSION   DBNGZW010000288 DBNGZW010000000
VERSION     DBNGZW010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1790711"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="AC16JC_06910"
     CDS             <1..868
                     /locus_tag="AC16JC_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011462685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5164463.1"
                     /translation="SQVLPDHRRTHGFAWYSLAGSFATALGSLCGGVVSGALQHAAVS
                     PLGSYRVVVFAYAGLGVALTLLFGRLSTSVEVRSAIRAASGIGRSRRVIFRLSSLFAL
                     DAFAGGFVVQSFAAYWFYLRFGVNPATLGGIFLGANLFAGVSALLAARLAGRFGLVRT
                     MVFTHLPSNVLLILVPLMPTLPLAIAVLLLRFSISQMDVPTRQSYVMAVVSPEERSAA
                     AGITGVARTTGAAIAPLFAGWLFARPSLISLPFFIAGTLKVVYDVLLYRGFVALRAPE
                     ESPSPPRSAASS"
     gene            complement(982..1290)
                     /locus_tag="AC16JC_06915"
     CDS             complement(982..1290)
                     /locus_tag="AC16JC_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014086419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1272 domain-containing protein"
                     /protein_id="HIR5164464.1"
                     /translation="MLELRPTCENCNTALPPDSRDARICSYECTFCAACVEHVLDNVC
                     PNCGGGFVPRPIRPARNWKGDNFLGKDPGSLTVRHRPVDVAAHQRFADGIRGIPPEQR
                     "
     gene            1545..2774
                     /locus_tag="AC16JC_06920"
     CDS             1545..2774
                     /locus_tag="AC16JC_06920"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase domain-containing protein"
                     /protein_id="HIR5164465.1"
                     /translation="MHVLLVDHDEADAVRVRDALWSCRYDVTHCGQMASGLAQLETVP
                     IDVMLAALHLPDAEGVEVVHRVRGVARHLPLVVMAGPDDDALAEAAVRSGAQDCLPKA
                     GLGGPLIERSLRYACERARLEAALEEAETRLRIIQDRVPATICDLTPDGTVTGLNAEF
                     QAITGWPPAEWIGRDFVELLHPEDRPAALELLRRTVRNDAPPALDVRMRARQGGYVIA
                     SFSAVPVVKQGRVSSVVGIARDVTELRQLETRQRDTSRTDHLTGLGNRRACEEAITRE
                     VARAMRENASVVFVLFDLDHFKVVNDTHGHHVGDAVLRAVAGVLREASRSSDLVGRWG
                     GEELLAILPRTELWAARPFAERVRAAVAALDGLPCRVTVSAGMAEWTRGQDVPTVLAR
                     ADARLYEAKRAGRNRVC"
     gene            2803..3972
                     /locus_tag="AC16JC_06925"
     CDS             2803..3972
                     /locus_tag="AC16JC_06925"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5164466.1"
                     /translation="MSDASPRCAACRWPLGPEDLFCPRCGTPTPLNVDPEATLVTPGT
                     AAAAARASDGTMAAPDGAAGSPPRPSGSTFTDRLRHVLGAEYELLSVIGQGGFARVYR
                     VRDRRLDRIVAVKVIRPDLIGTQEFVQSFRNEGVALAKVRHPGIVPIYDIRERGGLIY
                     YIMPLVEGTTLEVRMQRARLPPYESRRILCELADALAAAHRAKMVHLDIKPANVFLEG
                     DLQKVLLMDFGIARALTGKLEDAAGGAVVGTPEYMSPEQARGLRGIDHRSDIYSLGAL
                     GYRMLMGRPPFRGRDAADILAKHINEAPVPLRDINPTIQKDFADAIMRCLQKDPQDRF
                     PTAMELRGALESVTFFGARAEPTAGEGVSAVPRTLLALIAAAAFLLGLVVGQVLR"
     gene            complement(4028..>4331)
                     /locus_tag="AC16JC_06930"
     CDS             complement(4028..>4331)
                     /locus_tag="AC16JC_06930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164467.1"
                     /translation="AGKKYVLTGPEAVSYHDVAAALADATGRAVTYRPISMDEARTRL
                     AARGIEPDLIDAMLAIAAYQQAGGPTSVVSPTAERLLGRPPRTIRDFARDHARHFT"
BASE COUNT          570 a         1494 c         1582 g          685 t
ORIGIN      
        1 gtcacaggtg ctgcccgacc acaggcggac gcatggcttt gcctggtact ccctggccgg
       61 ctcgttcgct accgcgctcg gctcactgtg cggcggtgtc gtgtccggcg cgctgcagca
      121 tgcggccgtg tcgccgctcg ggagttatcg cgtggtggtc ttcgcctacg cagggctggg
      181 agtggcgctc accctgctgt tcggccgcct ctcgacgtcg gtggaagtgc ggtccgcgat
      241 ccgggcagcc tccggcatcg gccggtcacg ccgggtgatt ttcagactct ccagcctgtt
      301 cgctctcgat gcgttcgccg gcggcttcgt cgtgcagagt ttcgcggcgt actggttcta
      361 cctccggttc ggtgtgaacc ccgcgacgct ggggggcatc ttcctgggcg cgaacctctt
      421 cgccggcgta tcggccctgc tcgccgcccg gctggcgggg cgcttcggcc tggtgcggac
      481 catggtgttc acgcacctgc cgtccaacgt cctgctcatc ctcgtgccgc ttatgccgac
      541 tctgcccctc gccattgcgg tgctgctgct gcggttcagc atcagccaga tggacgtgcc
      601 gacgcggcag tcctacgtca tggccgtcgt gagccccgag gagcgctccg ccgcggccgg
      661 catcacgggc gtcgcgcgaa cgaccggcgc ggcgatcgcc ccgctcttcg ccgggtggct
      721 gttcgcccga ccttcgctca tctcccttcc cttcttcatc gcgggaacgc tcaaggtcgt
      781 ctacgatgtg ctgctctacc ggggattcgt ggcgctacgg gcaccggagg agtccccctc
      841 tccccctcgc tccgctgcct cgtcttagtt ccccctctcc cgattccttg gttccccctc
      901 tccctataga gcctgccccg agcgcagcga gggggagagg gggttagggg gtggggtacc
      961 cgagcgggcc aggcgcgacg actagcgctg ctcgggtgga atccccctga tcccgtcggc
     1021 gaagcgctga tgggcggcaa cgtccacggg ccggtgtctc accgtcaggc tcccggggtc
     1081 cttgccgagg aaattgtcgc ccttccagtt cctggcgggc cgaatgggcc gtggcacgaa
     1141 cccgccgccg caattcgggc acacattgtc caaaacgtgc tcgacgcacg cagcgcagaa
     1201 cgtgcattcg taggagcaga tccgggcgtc ccgggagtcg gggggcaacg cggtattgca
     1261 gttctcgcac gtgggacgaa gctcgagcat gcacaactct agcacgcgag tcccgcgtga
     1321 tgcgaccggc atcgtgagga atccttgcgg gttccgtggc gggcactgat ccgacccgtg
     1381 cgggtgaagc cggccggccg gtgctgggcc acgacgagcg ggggcgaccc gcatcggccc
     1441 tcctgcccat gttaaaatga agccatggcc ggaccggcgt gaccggtgcg ggagtcgcac
     1501 gactcgttgg gggccggcca cgatagccga tcggagaatg ccgggtgcat gtactgttgg
     1561 tggatcacga cgaagctgac gccgtgcggg tgcgcgatgc cttgtggtcg tgccggtacg
     1621 acgtcacgca ttgcgggcag atggcgagcg ggctggcaca gctcgagacc gtgccgatcg
     1681 acgtgatgct ggccgcgctc cacctcccgg acgcggaggg tgtggaagtg gtgcaccggg
     1741 tgcggggcgt cgcgcggcac ctgccgctgg tcgtcatggc gggaccggac gacgacgccc
     1801 tcgccgaggc ggcggtccgc agcggcgcgc aggattgcct gccgaaggcc ggcctcgggg
     1861 ggccgctgat cgagcggtcg ttgcgctacg cgtgcgagcg cgcgcgcctc gaggcggcgc
     1921 tcgaggaggc ggaaacgcgg ctgcgcatca tccaggaccg cgtgccggcg acgatctgtg
     1981 acctgacgcc ggacggcacc gtgacgggcc tcaacgccga gttccaggcg atcaccgggt
     2041 ggccgccagc cgagtggatc ggtcgggact tcgtcgagct gctgcacccc gaggaccggc
     2101 ccgcggcgct ggagctgctg cggcggaccg tgcgcaacga cgcgccgccg gcgctggacg
     2161 tccgcatgcg ggcccggcag ggcggctacg tcatcgccag cttcagcgcc gtccccgtcg
     2221 tgaagcaggg gcgggtcagc agcgtcgtcg ggatcgcgcg cgacgtcacg gagctccggc
     2281 agctcgagac gcggcagcgc gacacctcgc ggaccgacca cctcacggga ctcggcaacc
     2341 gtcgcgcgtg cgaggaggcc atcacgcgtg aggtcgcgcg cgcgatgcgg gagaacgcct
     2401 cggtagtgtt cgtgctgttc gacctggacc acttcaaggt ggtgaacgac acgcatggcc
     2461 accatgtcgg cgatgcggtg ctgcgcgccg tcgcgggggt cctccgggag gcgagccgct
     2521 ccagtgacct ggtgggacgg tggggcggcg aggagctgct ggcgatcctg ccgcgcaccg
     2581 aactgtgggc cgcccgcccc ttcgccgagc gcgtccgggc agccgtggcc gcgctcgatg
     2641 ggctgccctg ccgcgtgacc gtgtccgccg ggatggccga gtggacgcgc gggcaggacg
     2701 tgccgacggt gctcgcccgc gccgacgcgc ggctctacga ggcgaagcgc gccgggcgga
     2761 accgcgtctg ctgagcgcca ccgggcgcag agatttggtg tcatgagcga cgcgtctccg
     2821 cgctgcgcgg cctgccgctg gcccctgggc cccgaggacc tgttctgtcc ccggtgtggc
     2881 acgccgacgc ccctcaacgt ggatcccgaa gccacgctcg tgaccccggg gaccgctgcg
     2941 gcggcggccc gggcgagcga tggcaccatg gccgcgccgg acggcgcggc gggatcaccg
     3001 ccgcggccct ccgggagcac cttcaccgac cggctgcgcc acgtgctcgg cgccgagtac
     3061 gagctcctct cggtgatcgg ccagggcggc ttcgcccgcg tctaccgggt cagagaccgg
     3121 cggctcgacc gcatcgtggc cgtcaaggtc atccggcccg atctgatcgg gacgcaggag
     3181 ttcgtgcaga gcttccgcaa cgaaggcgtc gccctggcga aggtgcggca ccccgggatc
     3241 gtccccatct acgacatccg cgagcggggc gggctgatct actacatcat gccgctggtg
     3301 gaggggacga ccctcgaggt gcgcatgcag cgggcgcgcc tgccgcccta cgagtcgcgg
     3361 cgcattctct gcgagctggc cgacgccctg gcggcggccc accgggccaa aatggtccac
     3421 ctggacatca agccggccaa cgtgtttctg gagggagacc tccagaaggt gctgctgatg
     3481 gatttcggga tcgccagggc actcaccggg aagctggagg acgcggcggg cggggcggtc
     3541 gtggggacgc ccgagtacat gtcgccggag caggcacgcg gactccgggg catcgatcac
     3601 cgctcggaca tctactcgct gggcgccctc ggctatcgca tgctcatggg gcggccgccg
     3661 ttcagggggc gggacgccgc cgacattctc gcgaagcaca tcaatgaggc gccggtgccc
     3721 ctgcgcgaca tcaacccgac gattcagaag gatttcgcgg acgcgatcat gcgctgcctg
     3781 caaaaggatc ctcaggaccg gttcccgacc gcgatggagc tgcgcggcgc cctcgagtcg
     3841 gtgacgttct tcggcgcgcg ggcggaaccg acggccggcg agggcgtctc ggccgtgccc
     3901 cgcaccctcc tggccctcat cgccgccgcc gcgtttctcc tcgggttggt cgtcggtcag
     3961 gtgctccggt aacggagcgc gcccggatcc gcttcgtgcc gctccgggcg gcgggggtgc
     4021 gggaggatca ggtgaagtgc cgggcgtggt cccgggcgaa atcgcgaatc gtgcgcggcg
     4081 gccgccccag caaccgctcg gccgtcggcg agaccaccga cgtcgggccg cccgcttgct
     4141 ggtaggcggc gatggccagc atcgcgtcga tgaggtccgg ctcgatcccg cgcgccgcga
     4201 ggcgcgttcg tgcctcgtcc atggagatcg gccggtaggt caccgccctg cccgtggcgt
     4261 ccgccagggc ggcggcgacg tcgtggtagg agacggcctc gggaccggtc aggacgtatt
     4321 tcttgcccgc g
//
LOCUS       DBNGZW010000289         8462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_844028, whole genome shotgun sequence.
ACCESSION   DBNGZW010000289 DBNGZW010000000
VERSION     DBNGZW010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8462
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_844028"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1706)
                     /gene="dnaE"
                     /locus_tag="AC16JC_06935"
     CDS             complement(<1..1706)
                     /gene="dnaE"
                     /locus_tag="AC16JC_06935"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR5164468.1"
                     /translation="MAFVHLHTHSEYSLLDGANRIPDLLDRIVALGMDSLAITDHGNL
                     HGAWTFYEEAKARKVRAILGFEAYLAFGSRHAREKPADAPGPYSHLVLLAKNRAGYRN
                     LVKLSSIGFLEGFYRRPRIDREVLERHKEGLVCLAACLSGEIALYLRAGQYAAAKASA
                     EYFARLFGPDGFWLEVQNHGIAEEETVTAGMFRLSEELGIPVVATNDAHYLRKDDAEA
                     HDVLLAIGTGKDLDDPKRFRFFGSESYVKSEKEMKPLFPGRPEVLAETARVAELCEFD
                     FEKRYFLPQYPRPAEFATDNDLLVHLARAGARERYGDPLPPAVEERLAFELDVIQRTG
                     YAGYFLIVYDLIKAARDRGIPVGPGRGSAAGSLTAYALRITSVDPLRFDLLFERFLNP
                     ERISMPDIDLDFCFERRGEVIEYVRARYGRESVGQIITFGTMKARAAFRDVARTLRLE
                     IGEVDRTTKLIPAGPAAMWTLAEAAQKVPELRAITEQDERVRKVIDLGSRIEGLARHA
                     SVHAAGVVIAPGPLTDYVPVCLAPQDDEAIITQYDMVALEKVGMLKIDLLGLRTLTVI
                     HDT"
     gene            1792..3066
                     /locus_tag="AC16JC_06940"
     CDS             1792..3066
                     /locus_tag="AC16JC_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164469.1"
                     /translation="MRTAVRLLALALVLALVYKYAAGAGPELRAALALGVLVVAAELA
                     GRLALRLRLPRVSGFVAAGLVLRANWSINRMDELETLQFVADAALALFALRAGLAWRS
                     GGNGATSGLGRYLASSLVLPFALTAAVVYALHPWFPLTVHQPSRDALAVALVLGAFTA
                     VAAPALVWVTLTDGPAGPLGDVLLRLHALRDIGAVLLFAAALALARPLASAGALQPLA
                     FAPPLAALGAAALAGALLAALAGRFRRLLGTDPGIFTLAVAFGAALAARAGPAEVTLA
                     ALVAGIGLSRFDAETAAALRTRFDVHGAALAAGAFALFGARLDPSVLFEVWPWVVGLA
                     ILRTAGLYWGGRLAARGGALSGTDDLVRSGWLGLIGQAGVGLLLAAAGRRAFPEWGVS
                     FEGLAVALVALHAVVGPICLRRALARGPALTE"
     gene            3085..4971
                     /locus_tag="AC16JC_06945"
     CDS             3085..4971
                     /locus_tag="AC16JC_06945"
                     /inference="COORDINATES: protein motif:HMM:NF014427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5164470.1"
                     /translation="MKHRGMIALGVLGICACSGNDGVKVSVTSITVTPAVATVLVGWT
                     LRLDATPKDASGIPLGGQAVSWRSVNPSIATVDASGLVTGVAVGQTRIIASTGNLSDT
                     AIVDATQDITIGTGEYASLAPLATSGAITFPANASTTDSVLYMIVPQIATASEGAVAA
                     FNLTGGALPLAALVRGGTSATATDPGIRFHDFLRQQERNQYAALVVPSALRGEVAPAA
                     GIELAPPTVGSKRSFKVCGDLSCSTLPTVSATAMVVTGHLAIYVDDAAPANGLSQADL
                     DALGAEFNTKLYAVDTTAYGRESDIDGNSVVIVLMSNVVNQLVTANQCQNQGFVAGFF
                     FGADIDPHFASQFNHGEVFYSIVADSTGSLSCPHTVSQLKRLVPVTFVHEFQHMISYN
                     QHVLVRNGSPQATWLDEGMSHFAEELGGRSFLPNDQASFSTFLINDLLNAYLYLDSSG
                     TNFLAFPASIGTLAQRGAVWLFVRQLADQYAKSTSLTDVARFTRSLTQTSLTGGDGVE
                     NATGASFENLVERWALANYVSGLPGFTPPPELTYKSWDFRTTFASLNQQLPSRFPKPF
                     PLTPPLSAGSTVALSDTLRAGSPWYLLVGQAASEPGFTLQFTGPSGPLPAALLPRLSV
                     IRLR"
     gene            4976..5914
                     /locus_tag="AC16JC_06950"
     CDS             4976..5914
                     /locus_tag="AC16JC_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR5164471.1"
                     /translation="MRLRRLPGRTVCCAMALVTRLAAQSGGSVVPELAGSTRSAGLGG
                     AGVALIGDAGALFANPAGIAAVRHLAVEGSLEPYLAGSTYSAAALALHAGRLAVGFGA
                     QALDYGSEPLIVPDSAAGGRRGIPTGGSFTAADLLGTAGIVYRRGFLAFGGAVKYARM
                     QIGDWSADAWGGDVGVAIAVFDIAALGVSVQNLGGDFGASGQGARLPRRTRAGLTMNY
                     VDPQGTFRLLTTLEGQWPADAPARLATGVEGGVVTHGVGMVGRIGFVTRSPTSAASRW
                     SVGGGVELRRLHLDYAYQGYSVVGGGTHRFGIRWAT"
     gene            5902..7020
                     /locus_tag="AC16JC_06955"
     CDS             5902..7020
                     /locus_tag="AC16JC_06955"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HIR5164472.1"
                     /translation="MGDLVERRRPRWGRVLKRLAAWVALGVPTAIGVALLVSADARYL
                     ARAAVEEARILLKRRAIAALVADSAVAPEVRERLRLVLDARAFAADSLQLAVGETYTT
                     YVDVGRDTLLLVLSGASRDRLRPYTWTYPIVGVVPYKGFFSPRLARVAAEELEREHYD
                     TYLRPAGAFSTLGYFSDPLFSTALDRDTMELVATVIHEVAHNTLYLKSQTPFNESFAS
                     FVGYRGAEAFFRARGDTLDAQRAAARWGDERTLDVFWAELARRLDSAYTGARTPEAVE
                     AARGSLFGWAREQLTGPVGQSLETYDWRWFAQAPLNNAVVIAQRIYRQNLNLFDELYA
                     AAGGNVAETIRAVQVRVFTQSDGDPYRALYSRVGTPQD"
     gene            7028..7450
                     /locus_tag="AC16JC_06960"
     CDS             7028..7450
                     /locus_tag="AC16JC_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007457030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5164473.1"
                     /translation="MELTRGIDIQAPPDAVWAVWSDVERWPEWTASVSRIERLERGPL
                     AVGHRARVRQPKLPTAMWLVTKLEVGRGFEWVSSSPGAHVTGYHWIEPQGSGSRVRLG
                     IVFAGPIARLVGWVARSLSERYLELEANGLKARSEGRG"
     gene            complement(7451..>8462)
                     /locus_tag="AC16JC_06965"
     CDS             complement(7451..>8462)
                     /locus_tag="AC16JC_06965"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR5164474.1"
                     /translation="DHDEVLGLATIMTWKTALMDIPFGGAKGGVAVDPRALSTLELER
                     LTRRFTQRIAIVLGPYRDVPAPDVNTNAQVMAWILDEYSSRRGYTPAAVTGKPVSLGG
                     SLGRDEATGRGVMYVMMEFARDFGVPLKGGRVVIQGFGNVGGHLARLLVAEAGATVVA
                     VSDVDGGIVNEGGLDIPGLLAHTAQRRPVSTWKDGRAIGTDELWSITCDWLVPAALGG
                     AITREQNAKTIDARVVVEGANAPTTPTADLILSERGIPVIPDFLANAGGVTVSYYEWA
                     QNLQQYRWTHEKVNDELRATITRAYAAVRDLAQQRGVSFRTAAYAIALQRVAEAERLR
                     GN"
BASE COUNT         1147 a         2941 c         3006 g         1368 t
ORIGIN      
        1 gtgtcgtgga tgacggtgag ggtgcgcagc ccgagcaggt cgatcttgag catcccgacc
       61 ttctcgagcg ccaccatgtc gtactgcgtg atgatcgctt cgtcgtcctg cggggcgagg
      121 cacaccggca cgtagtcggt gagcgggccc ggggcgatca ccacgccggc ggcgtgcacc
      181 gatgcgtggc gtgccagccc ttcgatccgg ctgcccaggt cgatcacctt gcggacgcgc
      241 tcgtcctgct cggtgatggc gcgcagctcg ggaaccttct gcgccgcctc cgccagcgtc
      301 cacatcgccg ccggacccgc cgggatgagc ttcgtggtgc gatccacttc cccgatctcg
      361 aggcgcagcg tgcgcgccac gtcgcggaac gcggcgcggg ccttcatcgt cccgaaagtg
      421 atgatctgcc ccaccgattc gcgcccgtag cgcgcccgga catactcgat cacttcgccg
      481 cggcgctcga agcagaagtc gaggtcgatg tccggcatcg agatccgttc gggattgagg
      541 aaccgctcga acagcagatc gaagcggagc gggtcgacgc tggtgatgcg caacgcgtag
      601 gcggtgagcg agccggcggc cgagccgcgc cccggcccca ccgggatgcc gcggtcgcgg
      661 gccgccttga tgagatcgta gacgatcagg aagtacccgg cgtagccggt gcgctgaatc
      721 acgtccagct cgaaggccag ccgctcctcg accgccgggg gcagggggtc gccgtagcgc
      781 tcgcgcgccc ccgcgcgcgc gaggtgcacc aggagatcgt tgtcggtcgc gaactccgcc
      841 gggcggggat actgcggcag gaagtaccgc ttctcgaaat cgaactcgca cagctcggcc
      901 acccgcgccg tttcggccag cacctccggc cggcccggga acagcggctt catctccttt
      961 tcgctcttga cgtagctctc cgacccgaag aagcggaacc gcttggggtc gtccaggtcc
     1021 ttgcccgtgc cgatggcgag cagcacgtcg tgcgcctcgg cgtcgtcctt gcgcaggtag
     1081 tgggcgtcgt tggtggctac gaccgggatg cccagctcct cggaaaggcg gaacatgccg
     1141 gcggtgacgg tctcctcttc ggcgatgccg tggttctgca cctcgagcca gaagccgtcg
     1201 gggccgaaca ggcgcgcgaa gtactcggcg ctggccttcg cggccgcgta ctggcccgcg
     1261 cgcaggtaga gcgcgatctc cccggacagg cacgcggcca ggcagaccag tccctccttg
     1321 tgccgctcca gcacttcgcg gtcgatacgc ggccggcgat agaaaccctc gaggaagccg
     1381 atcgacgaca gcttgacgag gttgcgatac cccgcgcggt tcttggccag caggacgaga
     1441 tgggagtagg ggccgggggc gtcggcgggc ttctcgcggg cgtgccggct cccgaaggcc
     1501 aggtacgcct cgaaacccag gatcgcgcgg accttccggg ccttcgcttc ttcatagaag
     1561 gtccaggcgc cgtggaggtt gccgtggtcg gtgatggcga gggagtccat ccccagcgcc
     1621 acgatccggt ccagcaagtc cgggatgcgg ttggcgccgt cgaggagaga gtattcgctg
     1681 tgggtatgga gatgaacgaa ggccatcgcg ccgaccgcgg aatcaagcca agatacgggc
     1741 gccggctcgc attgtcaaaa cccgttgcgg cgtcgaaatt tcgcccactc catgcgcacc
     1801 gccgtccggc tgctcgccct cgcgctcgtc ctggcgctgg tctacaaata cgcggccggg
     1861 gcgggccccg agctccgggc cgcgctggcc ctgggagtgc tggtcgtggc tgccgagctc
     1921 gcgggccggc tcgcgctgcg tttgcggttg cctcgcgtgt cgggcttcgt cgcggccggc
     1981 ctggtgctgc gcgccaactg gtcgatcaat cgcatggacg agctggagac gctgcagttc
     2041 gtcgccgatg ccgcgctggc gctgttcgcg ctgcgcgccg gtctcgcgtg gcgcagcggc
     2101 ggcaacggcg ccacctccgg cctgggacgc tatctcgctt cttccctggt gctgccgttc
     2161 gccctcacgg ccgccgtcgt atacgcgctg catccctggt ttcccctcac cgtgcatcag
     2221 ccgtcccgcg acgcgctggc ggtggccctc gtactcggcg ccttcaccgc cgtggcggcg
     2281 ccggcgctcg tgtgggtgac cctgaccgac ggccccgccg ggcccctggg ggacgtgctg
     2341 ctgcggctcc acgccttgcg cgacatcggc gcggtgctgt tgttcgccgc ggccttggcg
     2401 ctggcgcggc cgctcgcgag cgcgggcgcg ctgcagcccc tcgcctttgc cccgccgctg
     2461 gccgcgctcg gcgccgcggc gctcgccggt gcgttgctcg cggccctggc gggacggttc
     2521 cgccggttac tgggcacgga ccccggaatc ttcacactgg ccgtggcgtt cggcgccgcc
     2581 ctggcggcgc gggccgggcc ggccgaagtg acgctggcgg cgctggtggc agggatcggg
     2641 ctgtcgcgct tcgacgccga gacggccgcg gcgctgcgga cccggttcga cgtccacggg
     2701 gctgcgctgg cggccggtgc cttcgcgctg ttcggggcgc gtctcgaccc gtcggtgctc
     2761 ttcgaggtct ggccctgggt ggtggggctc gcgatcctcc gcaccgccgg gctctactgg
     2821 ggaggtcgcc tggccgcacg cgggggagcg ctgtcgggga ccgacgacct ggtgcgctcg
     2881 ggatggctcg ggctcatcgg gcaggcggga gtaggtttgc tgctcgcggc cgcgggtcgt
     2941 cgggctttcc ccgaatgggg tgtatcattc gagggtctgg cggtggcgct cgtcgcgctg
     3001 cacgcagtgg tcggtccgat ctgcctgcgt cgcgcgctcg cgcgagggcc ggcgctcacg
     3061 gaatgagtca gcgggggagg taacgtgaag caccgcggaa tgatcgccct gggggtgctc
     3121 gggatctgcg cctgctcggg aaacgatggt gtgaaagtgt cggtcacctc gatcacggtc
     3181 accccggcag tggcgacagt gctggtggga tggacattgc ggctcgatgc gacgcccaag
     3241 gacgcgagcg ggataccact cggcggccag gccgtgagct ggcggagcgt caatccgtcc
     3301 atcgcgacgg tggacgcgag cgggctggtt acaggcgtgg ccgtggggca gacgaggatc
     3361 attgccagca ccggtaatct cagcgatacg gcgatcgtcg acgccaccca ggacatcaca
     3421 atcgggacgg gcgagtacgc gagcctggct cccctcgcga cctcgggcgc catcaccttc
     3481 cccgccaatg cctccaccac cgattcggtc ctgtacatga tcgtcccgca gattgctacg
     3541 gccagcgaag gtgcggtcgc ggcattcaac ctgaccgggg gcgccctgcc gctcgcggcg
     3601 ctcgtgaggg gcgggacgag cgcgacggcg accgacccgg gaatccgctt ccacgacttc
     3661 ctgcggcagc aggagcgcaa ccagtacgcc gccctggtgg tgccgtccgc cctcagaggg
     3721 gaggtcgccc ccgcggcggg catcgagctg gcgcccccga ccgtcgggag caaacggtcg
     3781 ttcaaggtct gcggtgacct gtcctgtagc acgttgccga cggtcagcgc caccgccatg
     3841 gtcgtgaccg gccatctggc catctacgtg gatgatgccg ccccggcaaa cggtcttagc
     3901 caggccgatc tcgatgcgct cggcgcggag ttcaatacga agctgtacgc cgtcgatacc
     3961 acagcctacg gtcgcgagtc ggacatcgac ggcaactccg tggtcatcgt gctcatgagc
     4021 aatgtcgtca accagctggt gacagcgaac caatgccaga atcagggatt cgttgccggc
     4081 ttcttcttcg gagctgacat cgatccgcac ttcgccagcc aattcaacca tggcgaggtg
     4141 ttctactcga tcgtggcgga ctccaccggc tcgctctcct gcccccacac cgtgagccag
     4201 ctcaagcggc tcgtcccggt gacgttcgtc cacgagttcc agcatatgat cagctataac
     4261 cagcacgtgc tggtccggaa cggctcgccg caggcgacgt ggctggacga ggggatgtcc
     4321 cacttcgccg aggagctggg tggccgcagc ttcctgccga acgaccaggc atccttctcc
     4381 accttcctca tcaacgacct gctcaacgcg tacctgtacc tcgactcgag cggcaccaat
     4441 ttcctcgcgt tccccgcctc catcgggact ctggcccagc ggggggcggt gtggcttttc
     4501 gtgcgccagc tggcggacca gtacgccaag agcacgtcgt tgactgacgt cgccagattc
     4561 acccggtcgc tcacgcagac gagcctgacc ggtggcgacg gcgtggagaa cgcgaccggc
     4621 gcgtctttcg agaacctggt cgagcggtgg gcgctggcca attacgtgtc gggcctgcca
     4681 gggttcaccc cccctcccga gttgacgtac aagtcctggg acttccgaac gaccttcgcg
     4741 tcgctcaatc agcagctgcc ctcacggttc ccgaagccgt tcccgttgac cccgccgctt
     4801 tcggcgggct ctaccgtcgc gctcagcgac acactgcggg ccggatcgcc gtggtatctc
     4861 ctcgtcgggc aagcggcgtc ggagcccggg ttcacgctgc aattcaccgg accgagcggc
     4921 ccactgccag cggcgttgct gccgcggctg agcgtgatcc ggctccggta gcgacgtgcg
     4981 gctccgccgt ctccccggcc ggacggtctg ctgcgcgatg gccctcgtca cgcgcctggc
     5041 cgcccaatcg ggcggcagcg tcgtccctga gctggccggg tcgacccgct cggcgggact
     5101 gggcggcgcc ggggtcgccc tgatcggcga tgcgggcgcc ctcttcgcca atccggcggg
     5161 gatcgccgcg gtccgtcacc tcgccgtaga aggctcgctc gagccctacc tcgccggctc
     5221 gacctacagc gccgccgcgc tggcgctgca cgccggtcgc ctcgccgtgg ggttcggcgc
     5281 gcaggcgctg gactacggtt ccgagcccct catcgtcccc gattccgccg ccggcgggcg
     5341 tcgcggtatc ccgaccggcg gcagcttcac cgccgccgat ctcctcggca ccgcgggcat
     5401 cgtgtaccgc cgcgggttcc tggcgttcgg cggggcggtg aagtacgccc gaatgcagat
     5461 cggcgactgg tcggcggatg cgtggggcgg cgacgtggga gtcgcgatcg ccgtgttcga
     5521 catcgcagcg ctcggcgtga gcgtgcaaaa cctcgggggc gacttcggcg cctcggggca
     5581 gggggcccgg ttgccgcgac gcacgcgcgc cggcctcacg atgaactatg tggaccccca
     5641 ggggaccttt cggctgctga cgaccctaga ggggcaatgg ccggcggacg ccccggcccg
     5701 gctcgcgacc ggcgtggaag gcggcgtggt cacgcacgga gtcggaatgg tcggacggat
     5761 cggcttcgtc acgcgctcgc ccaccagcgc ggcgtcgcgc tggagcgtgg gcggcggcgt
     5821 ggagctgcgc cggttgcacc tggattatgc ctatcagggg tactccgtcg tgggaggcgg
     5881 gacgcaccgc ttcgggatcc ggtgggcgac ctagtggaac ggcgccggcc ccgctggggg
     5941 cgcgtgctga agcggctcgc ggcgtgggtc gcgctgggtg tgccgacggc catcggggtg
     6001 gcgttgctcg tctcggccga cgcccgctac ctggcccggg ccgccgtcga agaagcgcgc
     6061 atcctgctca agcggcgggc gatcgccgcg ctcgtcgccg attcagcggt cgcccccgag
     6121 gtgcgcgagc ggctacgtct ggtcctcgac gcccgcgcct tcgcggccga ctcgctgcag
     6181 ctcgcggtcg gggagaccta cacgacctac gtggacgtcg ggcgggacac cttgctgctc
     6241 gtgctgtccg gcgcgtcgcg cgaccggctg cggccctaca cctggaccta cccgatcgtc
     6301 ggcgtcgttc cctacaaggg tttcttcagt ccccggctgg cccgcgtggc ggccgaggag
     6361 ctggagcgcg agcactacga cacctacctg cgtcccgccg gcgccttctc caccctcggg
     6421 tatttctccg atccgctgtt ctccacggcg ctggaccgcg acaccatgga gctcgtggcg
     6481 acggtgattc acgaggtggc gcacaacacc ctgtacctga agagccagac gccgttcaac
     6541 gagagtttcg cgagcttcgt gggctatcgc ggggcggagg cgttcttccg ggcgcggggc
     6601 gatacgctgg acgcccagcg ggccgcagcg cgctggggcg acgagcgcac gctcgacgtc
     6661 ttctgggcgg agctggcgcg gcggctcgat tcggcctaca ccggcgccag gaccccggaa
     6721 gcggtggagg cggcccgggg gtcgctgttc ggctgggcgc gcgagcagct cacggggccc
     6781 gtcggccaga gtctggagac ctacgactgg cgctggttcg cccaggcgcc gctcaacaac
     6841 gcggtggtga tcgcgcaacg catctaccgg cagaacctca acctgttcga cgagctctac
     6901 gcggccgccg gcggcaacgt ggcggagacg atccgggcgg tgcaggtgcg ggtgttcacg
     6961 cagtcggacg gggatcccta ccgggcgctc tactcgcggg tggggacgcc gcaggactga
     7021 tgccgtcatg gagctgaccc ggggcatcga catccaggcg ccccccgacg ccgtctgggc
     7081 cgtgtggagc gacgtcgagc gctggccgga gtggaccgcg agcgtctcgc gcatcgagcg
     7141 cctcgagcgg gggcctctgg ctgtcgggca ccgggcccgc gtgcgccagc cgaaactgcc
     7201 gaccgcgatg tggctggtga cgaagctcga agtgggccgt ggcttcgaat gggtgtcgtc
     7261 cagtcccggc gctcacgtga ccggctacca ctggatcgag ccccagggga gcggcagccg
     7321 ggtgaggctg ggaatcgtct ttgccggccc gatcgcgcgc ctggtcggct gggtggcgcg
     7381 gtcgctgtcg gagcgctacc tcgagctcga ggcgaacgga ctcaaagcgc gcagtgaggg
     7441 gcgggggtag tcagttaccc cgcagccgct ccgcctcggc tacccgctgc agcgcgatgg
     7501 cgtaggccgc ggtgcggaac gatactccac gctgctgcgc gagatcgcgc acggccgcat
     7561 acgccctggt gatcgtcgcg cgcagctcgt cgttgacctt ctcgtgcgtc cagcggtact
     7621 gctggaggtt ctgcgcccac tcgtagtagc tgacggtcac cccgccggca ttggcgagaa
     7681 agtccggaat cacggggatg ccgcgctcgg acaggatcag atcggccgtc ggcgtggtcg
     7741 gcgcattcgc cccctcgacc accacgcggg catcgatcgt cttcgcgttc tgctcccggg
     7801 tgatcgcacc gcccagcgcc gccggcacga gccagtcgca cgtgatggac cacaactcgt
     7861 cggtgccgat cgcccggccg tccttccagg tcgacaccgg gcgccgctgg gcggtatggg
     7921 cgaggaggcc cgggatgtcg aggccgccct cgttcacgat gccaccgtcc acgtcgctca
     7981 cggcgaccac cgtcgcgccg gcctcggcca cgagcaggcg ggccaggtgc ccgcccacat
     8041 tgccgaaccc ctggatcacc acccggccgc ccttgagcgg cactccgaag tcacgcgcga
     8101 attccatcat gacgtacatc acgccgcggc ccgtggcctc gtcgcgaccg agcgagccgc
     8161 cgagcgagac gggcttgccg gtcaccgcgg cgggcgtgta gccccggcgg ctcgagtact
     8221 cgtccaggat ccaggccatg acctgcgcgt tggtgttgac gtccggtgcc gggacgtcgc
     8281 ggtaagggcc cagcacgatg gcgatccgtt gcgtgaagcg ccgggtgagc cgctccagct
     8341 cgagcgtcga caacgcacgc ggatcgaccg cgacgccgcc cttggcgcct ccgaagggga
     8401 tgtccatcag cgcggtcttc caggtcatga tcgtggcgag gcccagcacc tcgtcatggt
     8461 cg
//
LOCUS       DBNGZW010000290         1797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1252355, whole genome shotgun sequence.
ACCESSION   DBNGZW010000290 DBNGZW010000000
VERSION     DBNGZW010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1797
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1252355"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            293..>1797
                     /locus_tag="AC16JC_06970"
     CDS             293..>1797
                     /locus_tag="AC16JC_06970"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5164475.1"
                     /translation="MTPDSPPPEVARPIRVLVADDAAAVRESSVAVLRLDGYDVTPCE
                     CADEALHLLERSRFDVVIVEEEMQRVDGLTVLRATRATSPNTRIIVTSGTPSVPAGEQ
                     AMRLGAWYYLPKPFTATQLHILVGLAAHPASLAVAPGPAAAARPIPLAPPAGPGVTPI
                     GRSPAFRSMIELAKRVARTDISVLLTGETGSGKEVLAQFIHQHSHRADRPMVSVNCSA
                     LPEALFESEVFGHRKGAFTGAVRDKPGLLEIAHNGTLFLDEVLEMPKPAQAKLLRVMQ
                     DGVVRRVGSESTDAIVNVRFIAATNGDPESAVRDGRLREDLYYRLNVVRLRVPSLRER
                     VEDIPLLANLFVSTHWRRNRPKNTPVPRLSERAIRALCEHRWRGNLRELQNVIEWATV
                     LLAPGQEVGPEDLHLVDRGRAAFTLMQCEDDLLDDPLPLARARFLAWFESRYLHWVIG
                     RAKGNIASAARLAGMDRATLYRMMNRRGLHRPKPPAWRASSMTVKHDEDPER"
BASE COUNT          277 a          641 c          613 g          266 t
ORIGIN      
        1 ggacaatctc gcctacaaac cgctcgaccc ggccacgttg ctgctgcggg cggggatggc
       61 gctgcgcgcc ggcgagcctc aggcgcggca ccaatggctg cgccgctacg tggagcggga
      121 tcaaccatga cgcgaatcgg ggcagccagc accgttccgc gacccacagt ggggcgcgcg
      181 cccgggtccc cgtccgcctg agcccgcgtg tatgtctcag cacgaaacga ggatgaccgc
      241 gccgccccac tccagttctg atctggcccg ctctgtgcct cgcactgggg cgatgacacc
      301 cgactcccca ccccccgagg tcgcccggcc gattcgcgtg ctcgtggcgg acgacgcagc
      361 ggccgtgcgc gaaagcagcg tcgcggtcct gcggctcgac ggctacgatg tcacgccgtg
      421 cgagtgcgcg gacgaggcgc tccacctgct ggagcgcagc cgcttcgatg tggtgatcgt
      481 cgaagaggag atgcagcgcg tcgacgggct gaccgtgctc cgcgcaacgc gggcgacgag
      541 ccccaacacc aggatcatcg tgacctcggg caccccgtcc gtgcccgccg gcgagcaggc
      601 gatgcgattg ggcgcctggt attacctgcc gaaaccgttc acggcgacgc agttgcacat
      661 cctggtcggt ctggccgctc atcccgccag cctggcggtc gcccccgggc cggccgccgc
      721 cgctcgcccg attcccctcg ccccacccgc cggtccgggc gtcacgccga ttggccgatc
      781 cccggccttc cgctcgatga tcgaattggc gaagcgcgtg gcccggaccg acatctcggt
      841 attgctcacg ggagaaaccg gcagcgggaa agaggtgctg gcccagttca tccaccagca
      901 cagccaccgt gcggaccggc cgatggtgtc ggtcaactgt tccgccctgc cggaagccct
      961 gttcgagtcg gaagtgttcg gacaccgcaa gggtgcgttc accggcgcgg tgcgggacaa
     1021 gcccgggctg ctcgagatcg cgcacaatgg cacgctgttc ctcgacgagg tgctcgagat
     1081 gcccaagccc gcccaggcga aactgctgcg ggtcatgcag gacggcgtgg tgcgccgggt
     1141 ggggagcgag tcgacggacg cgatcgtgaa cgtgcggttc atcgccgcca cgaacggcga
     1201 ccccgagagc gcggtgcggg acggccgtct gcgcgaggat ctctactacc gcctcaacgt
     1261 cgtccgcctg cgcgtgccgt cgctgcgtga gcgggtcgag gacatcccgc tgctcgccaa
     1321 cctgtttgta tcgacccact ggcgccgcaa tcgccccaag aacacgccgg tccccaggtt
     1381 gtcggagcgg gccatccgcg ccttgtgcga gcaccgctgg cggggcaacc tgcgcgagct
     1441 ccagaatgtg atcgaatggg cgacggtgtt gctggcgccc ggtcaggaag tcgggcctga
     1501 ggatctccat ctggtggacc gcggccgcgc cgcgttcacc ctgatgcagt gcgaggacga
     1561 tctgttggac gatccgctcc cgctggcgcg cgcgcgcttc ctcgcctggt tcgagtcgcg
     1621 atacctgcac tgggtcatcg gccgggcgaa gggcaacatc gccagcgccg cacgcctggc
     1681 cggcatggat cgcgcgacgc tgtaccggat gatgaaccgg cgcggcctgc acaggcccaa
     1741 acccccggcg tggcgcgcgt catccatgac cgtcaaacac gacgaggacc cggagcg
//
LOCUS       DBNGZW010000291         3842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_367848, whole genome shotgun sequence.
ACCESSION   DBNGZW010000291 DBNGZW010000000
VERSION     DBNGZW010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3842
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_367848"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1694
                     /locus_tag="AC16JC_06975"
     CDS             <1..1694
                     /locus_tag="AC16JC_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
                     /protein_id="HIR5164476.1"
                     /translation="VAIFSTLSIDKSATGYTLHASATGLTTASSTAFTITPGSATQLA
                     FTVQPTTTSAGGVITPAVQVTARDGFGNTATAFTGNVTIAIGANPGGGTLSGTTTVAA
                     IAGVATFSTLSIDQVGNGYTLTTAAGGLPGATSTPFDVTNTTLVFTVQPSTTTAGARI
                     TPAVQVTARNSLGQTITTFTGQVKVAIVPGTGTPGAILSGTTTVSAVAGVASFSTLSI
                     DKAGTGYRLSATATGFPSALSGTFTINVGAATKLGFIVPPSTTPAGATIIPAVQVAAQ
                     DAQGNTVKSFTGTVTMTISTNPSSGTLSGTTAVTLNAGVAAFSTLSINKAGVGYRLRA
                     TTSGLSNATSAPFDITPAAATQLIFTVQPISTTAGDVITPAVEVTAEDSMGNVVTGFT
                     GDVSVLITAGSGTTGATLSGTTTVTAVGGVAVFSDLSIDKAGTGYKLSATASGVAGKN
                     SASFQIVAGTPVRVNFSVQPATTTAGAIITPAVQVDVRDSLNNVVKTFTGEITVAIGV
                     NPGGGTLSGTTTLAAVAGVAVFSDLSIDLAATGYRLVATAAGLSSATSAAFSITP"
     gene            1788..2147
                     /locus_tag="AC16JC_06980"
     CDS             1788..2147
                     /locus_tag="AC16JC_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164477.1"
                     /translation="MPHQQWARLQTDVNVKLRRGAWYRVIKLGAVEAVLDVNRKPVSV
                     TRALLQIVPTPPSRWSVVPAPRNAHRFPTSWGERYGVCPACRGRSPLKGRPTSMRCTR
                     CNGLYEVAWNEPYLLSA"
     gene            2182..3411
                     /locus_tag="AC16JC_06985"
     CDS             2182..3411
                     /locus_tag="AC16JC_06985"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR5164478.1"
                     /translation="MTLSQPTPEPRRPSLVGGRATRPSIGFPVKVSRWRTFWQAPVPV
                     LITAGAGGEVLVARTRLVLTLLVLLLPLVDLLRRPGERGAAVYVGLGAALLGVALAAA
                     VLRLARKRVYTPPVGFATSLLDVTLVSAVLVMFLALGQPLSLVSNRVAFLVYFLAIAA
                     AALRFDPRICAVTGAGAMAEYAVVAFLARWLVGGAVEPVIWLDQVSRLALLGIAGLLS
                     TTLVVRTRELQWLSARDKLTGFLNRGIFDDLLNDASLRARRYGRPLSILMLDVDRFKQ
                     FNDTWGHAAGDEALKAVSAAIKQSVRQGEVVARYGGDEFVVLFPETRPETAVQRGEKI
                     RLRIPPIIAGGRGRQQTVPVTISVGVASLPADGVDGFEVLNQADMRLYEAKHAGRDAV
                     VGPAAQAEPPAAQAEAG"
     gene            complement(3408..>3842)
                     /locus_tag="AC16JC_06990"
     CDS             complement(3408..>3842)
                     /locus_tag="AC16JC_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164479.1"
                     /translation="HRLHEALDWGPDDGLLTFYIGKSAGAAVGALTFIRVDTPHGPLE
                     VAVGFRPDGTLHRVEVTKATVETKPWVLEAVRSGVLDRYAGVNAEAGDLGAVAKAPLV
                     PGGVEGYRGKVGQMAGYMLEQIDKGVQRAVVAYGWYYRPPNS"
BASE COUNT          559 a         1383 c         1329 g          571 t
ORIGIN      
        1 gagtcgccat cttctccacg ctcagcatcg acaagagcgc gacgggctac accctccatg
       61 ccagtgccac cggtctgacg acggcgagca gcaccgcgtt caccatcacc cccggctcgg
      121 cgacgcagct cgccttcacc gtgcagccga ctaccacgag cgctggaggg gtcatcaccc
      181 cggcggtcca ggtgaccgca cgagacggct tcgggaacac cgcgaccgcg ttcacgggca
      241 acgtgaccat cgcgatcggg gcgaatcccg ggggcggtac gctgtccggg acgacgaccg
      301 tggcggcgat cgccggggtc gccaccttct cgaccctcag catcgaccag gtcggcaacg
      361 ggtatacgct gacgactgcg gccggcggtc tgccgggcgc gacgagcacc ccgttcgacg
      421 tcacgaacac gacgctcgtc ttcaccgtgc agccttcgac gacgaccgcc ggcgcgagga
      481 tcacccccgc ggtccaggtg accgcccgca actcgctggg gcagaccatc acgacgttca
      541 ccgggcaggt gaaggtggcg atcgttcccg gaacgggcac gcccggggcc atcctgtccg
      601 gcacgaccac ggtgtccgcg gtcgcgggcg tcgcttcctt ctccaccctg agcatcgaca
      661 aggcgggcac cgggtaccgg ctgtcggcca cggcgaccgg cttcccgtcg gccctgagcg
      721 gtaccttcac gatcaacgtc ggcgccgcca ccaagctcgg cttcatcgtg ccgcccagca
      781 cgacgccggc gggggccacg atcatccccg ccgtccaggt cgccgcgcag gatgcgcagg
      841 gcaacacggt gaagagcttc accggcacgg tgacgatgac catcagcacg aacccctcga
      901 gcggcacgct gtcgggcacc accgcggtga cgctgaacgc cggtgtggcg gcgttctcga
      961 cgctcagtat caacaaggcc ggcgtcgggt accggctgcg ggcgacgacc agcggtttgt
     1021 cgaacgccac cagcgctccc ttcgacatca cgccggcggc cgcgacgcag ctgatcttca
     1081 ccgtgcagcc gatctcgacg acggccggag acgtcatcac gccggccgtc gaggtcaccg
     1141 ccgaagacag catggggaac gtcgtgacgg gcttcacggg cgacgtgtcg gtgttgatca
     1201 cggccgggag cggcaccacc ggcgcgacgc tctccggcac gacgacggtc acggcggtcg
     1261 gaggcgtcgc ggtattctcc gatctgagca tcgacaaagc ggggaccggt tacaaactgt
     1321 cggcgaccgc cagcggggta gccgggaaga acagcgcgtc cttccagatc gtggccggta
     1381 cgccggtccg ggtcaacttc agcgtgcagc cggcgacgac cacggcgggg gcgatcatca
     1441 caccggcggt gcaggtggac gtgcgcgact ccctgaacaa cgtggtgaag accttcaccg
     1501 gcgaaatcac cgtcgcgatc ggggtcaacc ccggtggggg caccctgtcc ggcacgacga
     1561 cgctggccgc cgtcgccggg gtcgccgtct tctcggacct gagcatcgac ctcgccgcca
     1621 cgggctatcg cctggtggcc accgccgccg gcctcagctc ggccacgagc gcggcgttct
     1681 cgatcacgcc ctgaggcgcg cgaggtcggc acctcgtccc gactcgtcac cgcttgacgc
     1741 gcgggcgtgg cgcgccaagc tttaggtcgc ttctccacga ggcgccgatg ccgcaccagc
     1801 aatgggccag actgcagacc gacgtcaacg tcaagttgcg ccggggagcg tggtaccggg
     1861 tcatcaagct gggggccgtg gaagcggtgc tggacgtgaa ccggaagccg gtcagcgtca
     1921 cccgcgcgct gctgcagatc gtcccgactc cccccagccg ctggagcgtg gtgccggcgc
     1981 cgcggaacgc gcaccggttt cccacgagct ggggagaacg ctatggcgtg tgccccgcct
     2041 gccggggacg ctcgccgctg aaaggtcgac ccaccagcat gcgctgcacc cggtgcaacg
     2101 ggctgtacga agtggcctgg aacgagccgt acctcctgag cgcctagcgc tccgccgctg
     2161 ttttcccgaa ttctccgacc gatgaccctg tcgcagccca cgcccgagcc gcgccgcccg
     2221 tccctggtgg gaggtcgcgc gacccgtccc tcgatcgggt ttcccgtcaa ggtcagccgc
     2281 tggcggacct tctggcaggc tcccgtgccg gtgctcatca ccgccggagc gggcggcgag
     2341 gtgctggtcg cgcgcacgcg actcgtgctc accctgctcg tcctgctgct tccgctggtc
     2401 gacctgctgc ggcggccggg ggagcggggg gcggcggtct acgtcggcct cggcgcggcc
     2461 ttgctcgggg tggcgctggc ggccgcggtc ctccgcctcg cgcggaagcg tgtgtatacg
     2521 ccgccggtcg gattcgccac cagcctgctc gacgtcaccc tggtgagcgc ggtgctcgtc
     2581 atgttcctgg cgctcgggca gccgctctcg ctcgtcagca accgggtcgc gttcctcgtg
     2641 tacttcctcg cgatcgccgc tgccgcgttg cggttcgatc cgcgcatctg cgcggtgacg
     2701 ggggcgggcg ccatggccga gtatgcggtg gtcgcgttcc tggcccggtg gctcgtgggc
     2761 ggcgcggtcg agcccgtgat ctggctcgat caggtgtcac ggctcgccct gctcggcatc
     2821 gccggcctgc tgagcaccac cctcgtggtg cggacgcgag agctgcaatg gctctcggcg
     2881 cgcgacaagc tcacggggtt cctcaaccgc ggcatcttcg acgatctgct gaacgatgcg
     2941 tccctgcgcg cgcgccgcta cgggcgcccg ctctcgatcc tgatgctcga cgtcgaccgg
     3001 ttcaagcagt tcaacgacac gtggggccac gcggcggggg acgaggccct gaaggccgtc
     3061 tccgccgcca tcaagcagtc ggtgcgccag ggagaagtcg tggcgcgcta cggcggcgac
     3121 gagttcgtcg tgctgtttcc cgaaacgcgt ccggagacgg ccgtgcagcg gggcgagaag
     3181 atccgcctgc gcatcccgcc catcatcgcc ggaggccgcg ggcggcagca gacggtcccc
     3241 gtgaccatca gcgtcggagt cgcgagtctg ccggccgacg gggtcgacgg cttcgaggtg
     3301 ctgaaccagg cggacatgcg gctgtacgag gcgaagcacg ccggccgcga cgcggtggtc
     3361 ggacccgccg cgcaggcgga gccgcccgcc gcccaggcgg aggccggtta actgttcggc
     3421 ggtcggtagt accagccgta ggcgacgacc gcgcgctgga cgcccttgtc gatctgttcc
     3481 agcatgtacc cggccatctg accgaccttc ccgcgatagc cttcgacgcc tcccggcacg
     3541 agtggcgctt tcgcgacggc gcccaggtca ccggcctccg cgttgacccc ggcataccga
     3601 tctagcacgc cggagcggac cgcctccagc acccagggct tggtttccac ggtggccttg
     3661 gtgacttcga cgcggtgcag ggtcccgtcg gggcggaacc cgacggccac ctcgagcggc
     3721 ccgtgcggcg tgtcgacccg gatgaacgtg agggcgccga ccgcagcgcc ggcgctcttg
     3781 ccgatgtaga aggtcagcag gccgtcatcg ggtccccagt cgagcgcctc gtgcaggcgg
     3841 tg
//
LOCUS       DBNGZW010000292         5960 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_163480, whole genome shotgun sequence.
ACCESSION   DBNGZW010000292 DBNGZW010000000
VERSION     DBNGZW010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5960)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5960)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5960
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_163480"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..706)
                     /locus_tag="AC16JC_06995"
     CDS             complement(86..706)
                     /locus_tag="AC16JC_06995"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR5164480.1"
                     /translation="MSERASRSLGLLVLLFGIGTAARAQGPATYLLSPHSRFEVATGK
                     RGFFSFVGHNHLIRARAVTGHVTYDPADPARSRVEIRVLAESLEVLTPPDTAEIRKVT
                     EAMRSDVLDVAHHPEIRFVSKAAEPIPGGIRLRGDLELVGVTREVSVDVHLEAGADTL
                     RATGTFGVAQTSFGIRPYSGGPGGLVRVADRVTFTFAAVALRSPAP"
     gene            761..2077
                     /locus_tag="AC16JC_07000"
     CDS             761..2077
                     /locus_tag="AC16JC_07000"
                     /inference="COORDINATES: protein motif:HMM:NF016196.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HIR5164481.1"
                     /translation="MTQTPTAPAAPDRVSGLSEAAARWSRPDDALRQTQLDAMKRRAT
                     GLVFLAAAVFIAARIVEASVPWVGYIRATAEASLVGGLADWFAVTALFRHPLGLPIPH
                     TAIVATRKDRLGRTLGNFVQTHFLARDVLAIRLRDLGVARRLATWLSEVENSRRIARA
                     VASGLAKTLESLADEDLRALVQAGVSDRIRAFRVAPALGRTLSLVVAGSRHQEILSEA
                     VRLAAQAISDNRELIREQVRAESPWWVPGVVDDKIYHRIVAAVERLLHEIGNNPSHPL
                     RAAFDTALRNFIDRLQHSADVIARAEAMKDEWLRDPTFARLSDQLWETTRRAILGYAT
                     RDAGATPGALERGIAAFGAAVLANETLLAEVDDAVVEVVASVVEGYRAEVADLIAQTV
                     SAWDPGQTSQRIELAIGRDLQFIRINGTLVGGLVGLVLYTIARLWR"
     gene            2105..3424
                     /gene="mgtE"
                     /locus_tag="AC16JC_07005"
     CDS             2105..3424
                     /gene="mgtE"
                     /locus_tag="AC16JC_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HIR5164482.1"
                     /translation="MLEDIYTESPAVQLWEEWPALSMEERVARFKQLPSHEADDFFLS
                     LEPLEQETLLVALPQGEQRLWLRLLPPDDAVDLIQETPAEERDRLLDLLDDTTRREVR
                     ALLAHAEDDAGGLMNPRFARIRPDMTVGEAFRYLRKQSPQVETIYYAYVLDGEQRLRG
                     VVSFRQLFQAPEDRSVADVMRTDLVTVPEHMDQEQVARLFQQHDLLALPVVNETGRMV
                     GIITFDDIADAIREEATEDIQKIGGVEALEAPYLTIDFVSLLKKRAGWLAALFLGEML
                     TATAMAHFEQEIAAAVVLALFVPLIISSGGNSGSQASTLVIRAIALGEVRLRDWWRVL
                     RRELGAGLGLGAILGSIGVLRILTWQAVGHTYGVHYFRIAATVGFSLVGVVLWGSVAG
                     SMLPFMLRKAGFDPASASAPLVATLVDVTGLVIYFSIASIVLRGTLL"
     gene            complement(3480..5066)
                     /locus_tag="AC16JC_07010"
     CDS             complement(3480..5066)
                     /locus_tag="AC16JC_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing radical SAM
                     protein"
                     /protein_id="HIR5164483.1"
                     /translation="MRKLRIGIIDLVTRGPTRALYARLMHANLASIMPQVVGTWCEAE
                     GHDVTLVCYTGLENLVDELPHDVDLVFIGAFSQAALLAYSLSNLFRSRGAVTVLGGPH
                     ARCYPEDAQKYFDYVLGFTDQGVIREVLRDCQAHRPEGLRLAASRQPTTLPGVRERWK
                     FIKPTLDKAPILKLVPMLASLGCPYTCSFCIDSVVAYQPFDFGEIADDLRFLLTQQKR
                     PRVGWHDPNFGVRFDEVMDAIESAAPPDSIDFVAESSLSLLSEAHLQRLQRNGFKAVL
                     PGIESWYDLGNKSKTGNRTGLDKVREVSEHVNMIMRYLPYVQTNFVLGLDGDAGVEPF
                     ELTKRFVDLSPGAFPGYSLLSAFGRAAPLNLDYQRAGRVLPFPFHFLNNNHAMNVRPK
                     NYAWPEFYDQVIGLTDYTFSWRAIANRFRATRTTIPRWMNVVRAISSEGFGRLAYYKE
                     VRRRLDSDRPFRRYFEQETNDLPQFFVERVRRDLGPLYQWLPVGALNHDSLAYLAAEE
                     AAGVPSGVTAVAGARRAVSA"
     gene            complement(5155..>5960)
                     /locus_tag="AC16JC_07015"
     CDS             complement(5155..>5960)
                     /locus_tag="AC16JC_07015"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5164484.1"
                     /translation="VSDQAWVVRNNVQIVEGHRPQSGRAEICVGRRIVGRFLHTGIGD
                     LLHFAGRDWAVVCHFSAGGSAFESEIWGENEQFMPVFRGEVFQSITFRLRDAAGYEDA
                     KRALEGDQRITVDAHRESDFYMQQSQLLSRILRILAMVITSIMAVGAIFGAVNTMYAS
                     VASRAPEIAVLLTLGYRPVSVLASFLVESAIIAAVGGLIGCLLSLPINGIVTSTTNWA
                     SFSEIAFSFRVTPLLLAVGMGFAVVMGVLGGFFPALRAARLPVIQALRA"
BASE COUNT          956 a         2019 c         2077 g          908 t
ORIGIN      
        1 gcggtggaat gaaggcaccg gcgcttactg gagcaacgta cggaacgccc tatgaagaaa
       61 cgcgttacgc gggatgaaga cagccctacg gcgcagggga gcggagcgcc accgccgcaa
      121 aggtgaacgt cacccggtcg gcgacccgga ccagaccgcc gggcccgccg ctatagggcc
      181 ggatcccgaa gctcgtctgg gctacgccga acgtcccagt cgcccgcagc gtgtcggccc
      241 ccgcctcgag gtgcacgtcc accgagactt cccgcgtcac gcccacgagt tccaggtccc
      301 cccgcagacg gatccccccg ggaatgggct cggcggcctt cgagacgaac cggatctcgg
      361 ggtgatgcgc cacatcgagc acgtcgctgc gcatcgcctc ggtcaccttc cgaatctccg
      421 cggtgtcggg cggcgtcagg acctccagac tctcggcgag gacgcggatc tcgacgcggg
      481 agcgggccgg atcggctgga tcgtaggtga cgtgcccggt gacggcgcgc gcccggatga
      541 ggtgattgtg ccccacgaac gaaaagaagc cccgcttgcc cgtggcgacc tcgaagcggc
      601 tgtggggaga gagcagatag gtcgcgggcc cctgggcccg cgcggccgtc ccgatgccga
      661 acagcaggac gagaagtccc aggctccgac tggcgcgttc gctcatggag tctcctaccc
      721 ccgggtcgat gggaggatgc cgcgctatct tggttctcac atgacgcaga cacccaccgc
      781 cccggcggca ccggaccgcg tctccggtct gtccgaggcc gccgcccgct ggtctcgtcc
      841 ggacgatgcg ctgcgtcaaa cgcagctcga cgcgatgaag cgtcgggcca ccggcctggt
      901 atttctcgcg gccgccgttt tcatcgccgc ccggatcgtg gaggcgagcg tgccctgggt
      961 aggatacatc cgggcgaccg cggaggcttc gctggtcggc ggcctcgccg actggttcgc
     1021 cgtgaccgcg ctgttccgcc atccgctcgg cctgccgatc ccccacaccg ctatcgtcgc
     1081 gacccgcaag gaccggctcg gccggacgct gggcaacttc gtccagaccc actttctggc
     1141 acgggacgtg ctcgccatcc ggctgcgcga tctgggcgtc gcgcgacggc tggcgacgtg
     1201 gctcagcgag gtcgagaaca gtcgccgcat cgcgcgggcg gtggcgagcg ggctcgccaa
     1261 gaccctggag agcctggcgg acgaggacct gcgcgcgctg gtccaggccg gggtgagcga
     1321 ccgcatccgc gccttccggg tcgcgcccgc cctgggccgc acgctgtcgc tcgtggtcgc
     1381 cggcagccgc catcaggaga tcctgagcga ggcggtgcgg ctcgcggcgc aggcgatctc
     1441 ggacaaccgc gagctgatcc gcgagcaggt ccgggccgag agtccgtggt gggtgccggg
     1501 agtggtggac gacaagatct accaccggat cgtcgcggcg gtcgagcggc tgctgcacga
     1561 gatcgggaac aatccctccc acccgctgcg tgccgcgttc gacacggcgc tgcggaattt
     1621 catcgaccgg ttgcagcact cggccgacgt gatcgcccgg gccgaggcga tgaaggacga
     1681 atggctgcgc gatcccacct tcgcccggct gtcggatcag ctgtgggaga cgacccggcg
     1741 ggcgattctg ggctatgcca cacgagacgc cggggcgacg ccgggagcgc tggagcgcgg
     1801 gatcgccgcc ttcggggccg ctgtgctcgc caacgagacg ctgctggcgg aagtggacga
     1861 cgcggtggtc gaggtcgtgg cttcggtggt ggaaggctac cgggccgagg tggcggatct
     1921 gatcgcgcag accgtttcgg cctgggatcc cgggcagacc tcccagcgta tcgagctggc
     1981 gatcggccgg gacctgcagt tcatccgcat caacggcacg ctcgtgggcg gcctggtcgg
     2041 tctcgtcctg tacaccatcg cgcgcctctg gcgctgacaa ccctcctcgc aactcgaggg
     2101 acgcatgctg gaagacatct acaccgagag ccccgccgtc cagctctggg aggagtggcc
     2161 cgcgctgtcg atggaggagc gcgtggcgcg cttcaagcag ttgccgagcc acgaggcgga
     2221 cgacttcttc ctgagcctgg agccgctgga gcaggagaca ctcctggtgg ccctgcccca
     2281 gggcgagcag cgactgtggc ttcggctgct cccgccggac gacgccgtcg acctgatcca
     2341 ggagacgccg gccgaggagc gcgaccggct gctcgatctg ctcgacgaca ccacgcggcg
     2401 cgaggtccgg gcgctgctgg cccacgccga ggacgacgcc ggcggcctga tgaacccgcg
     2461 cttcgcccgc atccggcccg acatgacggt cggggaggcg ttccgctatc tccgcaagca
     2521 atcgccccag gtcgagacga tctactacgc ctacgtgctg gacggcgagc agcggctgcg
     2581 cggcgtcgtc tccttccggc agctgttcca ggctcccgag gaccggagcg tcgccgacgt
     2641 gatgcgcacc gacctcgtca cggtgccgga gcacatggac caggagcagg tcgcgcggct
     2701 gttccagcag cacgacctgc tcgcgctccc ggtggtgaac gagacaggcc ggatggtcgg
     2761 catcatcacc ttcgacgaca tcgcggacgc catccgggaa gaggcgaccg aggacatcca
     2821 gaagatcggc ggcgtcgagg ccctggaggc cccctatctc accatcgact tcgtcagtct
     2881 cctgaagaaa cgggcgggat ggctcgcggc gctgttcctg ggcgaaatgc tgacggccac
     2941 cgcgatggcc cacttcgagc aggagatcgc agcggccgtg gtgctcgccc tgttcgtccc
     3001 gctcatcatc tcgagcggcg gcaactcggg ctcccaggcg tccacgctgg tgatccgggc
     3061 gatcgcgctg ggcgaggtcc ggctgcgcga ttggtggcgg gtgctgcggc gcgagctcgg
     3121 ggccgggctg gggctgggcg cgattctcgg gtcgatcggc gtgctgcgga tcctgacgtg
     3181 gcaggcggtg ggtcacacgt acggcgtgca ttacttccgc atcgccgcga ccgtcgggtt
     3241 cagtctggtc ggggtcgtgc tgtggggcag cgtcgcgggt tcaatgctgc cgttcatgct
     3301 ccgcaaggcc gggttcgacc cggcgagcgc ctccgcgcca ctcgtggcga cgctggtgga
     3361 cgtcaccggg ctggtgatct acttctcgat cgccagcatc gtcctgcggg ggaccctgct
     3421 ctaacccttc ccccttccct cttccccctt ccccggatga ggagggggag ggggaggtat
     3481 caggcggaga ccgcccgccg tgcgccggcg accgccgtca cccccgacgg cacaccggcc
     3541 gcctcctccg ccgcgagata ggcgagcgaa tcgtgattca gggctccgac cgggagccac
     3601 tgatacaacg gtcccaggtc gcggcgcaca cgctccacga agaactgggg caggtcgttc
     3661 gtttcctgct cgaagtaacg ccggaacggc cggtcgctgt ccaaacggcg ccgcacttcc
     3721 ttgtagtacg ccagccggcc gaacccctcc gacgaaatgg cgcgcaccac gttcatccag
     3781 cgcgggatcg tcgtccgcgt ggcgcggaac cggttggcga tcgcccgcca ggagaacgtg
     3841 tagtcggtca ggccgatcac ctggtcgtag aactccggcc acgcatagtt cttcggccgg
     3901 acgttcatcg catggttgtt gttcaggaag tggaagggga acggcagcac ccgtccggcg
     3961 cgctggtagt cgagattgag cggtgccgcc cggccgaagg ccgagagcag cgaatagccg
     4021 ggaaacgcgc ccggggacag gtccacgaac cgcttagtga gctcgaacgg ctccacccct
     4081 gcgtcgccgt ccagtccgag gacgaagttg gtctgcacgt agggcaggta ccgcatgatc
     4141 atgttgacgt gctcggagac ctcccggacc ttgtcgagtc ccgtgcggtt gcccgtcttc
     4201 gacttgttcc cgaggtcgta ccacgattcg atccccggca gcacggcctt gaagccgttg
     4261 cgctgcagcc gctggagatg ggcctccgac aggagcgaca ggctgctctc ggccacgaaa
     4321 tcgatgctgt cgggcggcgc ggcggactcg atggcgtcca tgacctcgtc gaaccggacg
     4381 ccgaagttcg gatcgtgcca cccgacgcgc ggccgcttct gctgtgtcag gaggaatcgc
     4441 aggtcgtcgg cgatctcgcc gaaatcgaac ggctgatacg ccaccacgct gtcgatgcag
     4501 aagctgcagg tgtacgggca gccgagactg gcgagcatcg gcacgagctt caggatcggc
     4561 gccttgtcga gcgtcggctt gatgaacttc cagcgctcgc gcacgcccgg cagagtggtc
     4621 ggctgacgcg acgcggccag ccgcaacccc tccggccggt gcgcctggca atcccgcagc
     4681 acctcgcgga tcacgccctg gtcggtgaag ccgagcacgt agtcgaagta cttctgggcg
     4741 tcttcgggat agcagcgggc gtgcggcccg ccgagcacgg tgaccgcccc gcgcgagcgg
     4801 aacaggttac tcaaggaata cgcaaggagg gcggcctggc tgaacgcccc gatgaagacc
     4861 agatcgacgt cgtggggcag ctcgtctacg aggttctcga gcccggtgta gcacaccagc
     4921 gtgacatcgt gtccctccgc ctcgcaccag gtccctacca cctggggcat gatgctggcg
     4981 aggttggcgt gcatgagtcg cgcgtacagc gcgcgtgtgg gtccccgggt gacgaggtcg
     5041 ataatgccga tgcggagctt gcgcacgggc tcgcttgtgg aaggaggtac agcaaaggtg
     5101 cccccgcggg cacgtccgcg ctagtccact cggcggaggg gtccgggacc ggggtcaggc
     5161 gcggagcgcc tggatcaccg gcagtctggc cgcgcgcagg gccgggaaga agccgcccag
     5221 cacacccatc acgacggcga atcccatacc cacggcgagc agcaacgggg tgacgcggaa
     5281 actgaacgcg atctcgctga agctcgccca gttcgtcgtg ctcgtgacga tgccgttgat
     5341 cggcagcgac aggagacacc cgatgaggcc gccgacggcg gcaatgatcg cggattcgac
     5401 gaggaagctc gcgagcacgc tcaccgggcg gtaccccagc gtcaacagca cggcgatctc
     5461 gggcgcgcgg gaggcgaccg aggcatacat cgtgttcacc gcaccgaaaa tcgcgccgac
     5521 cgccatgatg ctggtgatga ccatcgcgag gatccggagt atccggctga gcagctggga
     5581 ttgctgcatg tagaaatcgg attcgcggtg cgcgtccacc gtgatgcgct ggtcgccttc
     5641 gagggcacgc ttggcgtcct cgtacccggc ggcatcgcgc agccggaagg tgatggactg
     5701 gaacacctcg ccgcggaaca cgggcatgaa ctgctcgttc tcgccccaga tctcggactc
     5761 gaacgccgag ccccccgcac tgaagtggca caccaccgcc cagtcgcgtc cggcgaagtg
     5821 cagcaggtcg ccgatcccgg tgtggaggaa ccgccccacg atacgccggc cgacgcagat
     5881 ctcggcgcgc cccgattggg gccggtggcc ctcgacgatc tgcacgttgt tccggaccac
     5941 ccacgcctgg tcgctcaccc
//
LOCUS       DBNGZW010000293         3190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1661130, whole genome shotgun sequence.
ACCESSION   DBNGZW010000293 DBNGZW010000000
VERSION     DBNGZW010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1661130"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1266
                     /locus_tag="AC16JC_07020"
     CDS             <1..1266
                     /locus_tag="AC16JC_07020"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5164485.1"
                     /translation="DQHDSVTVALLAIGPEQISDSEAEPTEAELTRYYDAHKNLFKRP
                     AAAWLSFIAVPRRPAASDSAAALARADSLRTKAAASPAKFAEVAQRESADTGTGRQGG
                     DIGWIKRNQPNFDSLFLAGLRGLSVGQVSRPVRSSFGYHILRLDAAQGDSVHVRHILV
                     PIELVGAHRDSVEARADTLDRLVAERDSGWLLDSVALRLGIPVGHAPKLIEGDRMLLG
                     RYVIPDVSIWAFERKPGETSPVIEGEAAYYVFRLDSLIPAGVPRLAEVRQQVVIAARI
                     EKKKTLWAERARQIAATLAGTSDLVAAGAAHGLAVQRLGAFSRLRPPPILQLEPRVLG
                     TAFRLGVGQRSDVLIGENRAFILQPLARTTADSTAWVAQRDSQRESLMQAARQARVEA
                     YLAGLRQRAKVVDRRKDLFQQPQNATAGS"
     gene            complement(1286..1510)
                     /gene="tatA"
                     /locus_tag="AC16JC_07025"
     CDS             complement(1286..1510)
                     /gene="tatA"
                     /locus_tag="AC16JC_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR5164486.1"
                     /translation="MTHILILLVIALLVFGARRIPEIGSSLGQGIREFKKSLKEVASD
                     QPQPPLPPAPPTGTSSSLPPSGGEPKRLNQ"
     gene            complement(1534..1806)
                     /locus_tag="AC16JC_07030"
     CDS             complement(1534..1806)
                     /locus_tag="AC16JC_07030"
                     /inference="COORDINATES: protein motif:HMM:NF025572.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HIR5164487.1"
                     /translation="MAHGPRRPLFYLGVLASGFVLGGFLHLLLGRFLPAGAAKEFFTT
                     AVTPTFGPVHMDLLVLSIALGPVGLEVPLLSVLGVAIAYFVARSLF"
     gene            complement(1809..2798)
                     /locus_tag="AC16JC_07035"
     CDS             complement(1809..2798)
                     /locus_tag="AC16JC_07035"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012568.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR5164488.1"
                     /translation="MSEQAIRVSLSDIQRPVAGELDRVAAELRRIIAADFPIIADAND
                     HLLRMKGKLFRPTLLLLSHQAAGGGDSRVVTLAAVVELIHLATLVHDDSVDHSALRRG
                     MPTLNALFSHQVAVIMGDYLYSRAIIELVRVGDLDPLRVFARVTNEMTIGEMRQLEAH
                     DRMAFGEAEYDLLIRAKTASLLSGACEVGALTAPAPVREALARFGMLLGMAFQITDDL
                     LDYTQPEAVTGKPSGLDLKEHKVTLPLIAALQRLAPADRQIVVRLMDTSDPTDAQVAE
                     VIRLVDAGGGLDDARRRALDLAQRAEAELGALPPSPAREALRDSISYAVERRS"
     gene            complement(2800..>3190)
                     /locus_tag="AC16JC_07040"
     CDS             complement(2800..>3190)
                     /locus_tag="AC16JC_07040"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5164489.1"
                     /translation="IDENIEETDFQSHYDLGVAFKEMGLLDEAIAEFQKALRAPEGKL
                     RTSEALGVCFHDKGAFGVAESILRRALELPSKGDQERLGILYWLARALEEQGKPGEAR
                     DLYGRVFAVDIRFMDVGARVKALARAH"
BASE COUNT          513 a         1144 c         1077 g          456 t
ORIGIN      
        1 gaccagcacg actccgtgac cgtcgcgctg ctcgcgatcg ggccggagca gatctccgac
       61 tctgaggcgg agccgacgga ggcggagctg acccgctact acgacgcgca caagaacctg
      121 ttcaagcggc ccgcagccgc ctggctcagc ttcatcgccg tgccgcgccg tcccgccgcc
      181 agcgactcgg cggcggccct ggcccgcgcc gacagcctgc gcaccaaggc cgccgcctcg
      241 cccgcgaagt tcgccgaggt cgcccagcgc gaatcggccg acaccggcac cggacggcag
      301 ggcggagaca tcggctggat caagcgcaac cagccgaatt tcgactcgct gttcctcgcc
      361 ggtctgcgcg ggctctcggt cgggcaggtg tcccgcccgg tgcgttccag cttcggatat
      421 cacatcctcc ggctggatgc cgcccagggc gactcggtgc acgtgcggca catcctcgtt
      481 ccgatcgagc tggttggcgc ccatcgcgac tccgtggaag cgcgcgccga caccctggac
      541 cgcctggtcg cggaacggga cagtggctgg ttgctcgaca gtgtggcgct ccggctgggc
      601 atcccggtcg gccatgcacc caagctcatt gagggtgatc gcatgctgct gggccgctac
      661 gtgatccccg acgtcagcat ctgggcgttc gagcgcaaac cgggcgagac gagcccggtg
      721 atcgagggcg aggccgccta ctacgtcttc cggctcgatt ccctgatccc ggcgggcgtg
      781 ccccggctcg ccgaggtgcg ccagcaggtc gtgatcgcgg cgcgcatcga gaagaagaag
      841 acgctgtggg cggagcgtgc ccgccagatc gcggccacgc tcgcggggac gtccgatctg
      901 gtggcggccg gcgccgcgca tggcctggcg gtgcagcggc tcggggcgtt ctcccgcctg
      961 cgccccccgc cgatcctgca gctcgagcca cgggtgctcg gcacggcatt ccggctgggc
     1021 gtggggcagc gcagcgacgt gctcatcggc gagaatcgag cgttcatcct gcagccgctg
     1081 gcgcgcacga ccgccgactc gacggcgtgg gtcgcgcaac gggattccca acgggagagt
     1141 ctgatgcagg ccgcgcgcca ggcgcgcgtg gaggcgtatc tcgcggggtt gcggcagcgt
     1201 gcgaaggtcg tggatcgccg caaggatctc ttccagcagc ctcagaacgc gaccgccggc
     1261 agctagccgg cgggtcgccg gcgccctact ggttgaggcg cttcggctct cccccggacg
     1321 gcggcagcga cgacgacgtg ccggtcggcg gcgccggcgg caggggcggc tgcggctgat
     1381 ccgaggcgac ttccttcagg ctcttcttga actcccggat cccctgcccg agcgacgagc
     1441 caatctcggg aatgcgcctg gccccgaaga cgagcaaggc gatgaccagc agaatcagga
     1501 tgtgcgtcat cgagagatcc atatgccact cctctagaag agcgatcggg ccacgaagta
     1561 ggcgatcgcc acccccagca ccgacagcaa cggtacttcg agacccaccg gccccagtgc
     1621 gatcgacagc accagaaggt ccatatggac cgggccgaag gtcggcgtga ccgcggtcgt
     1681 gaagaactcc ttggcggcgc ccgccggcag aaaccgcccc agcaggagat gcagaaagcc
     1741 gcccagcacg aatcccgagg ccagcacgcc caggtaaaat aacggacggc ggggaccgtg
     1801 tgccacgctc agctccgccg ctcgacggcg tacgagatgc tgtcgcgcag cgcctcacgg
     1861 gccggggagg gcggcagcgc acccagctcc gcttcggccc gttgggccag gtcgagcgcg
     1921 cgccgacgcg cgtcgtcgag cccgccgccg gcatcgacca ggcggatcac ttccgccacc
     1981 tgggcgtcgg tcggatccga cgtatccatc agccggacga cgatctgccg gtccgccgga
     2041 gcgagccgct gcaacgcggc gatcaggggc agcgtcacct tgtgctcctt caggtccagc
     2101 ccggacggct tgccggtgac cgcctcgggc tgcgtgtagt cgagcaggtc gtcggtgatc
     2161 tggaacgcca tccccagcag catgccgaat cgggcgagcg cctcgcggac cggtgcgggc
     2221 gccgtcaacg caccgacctc gcaggcaccg gacaggaggg acgcggtctt cgcccggatg
     2281 agcaggtcgt actcggcctc gccgaaggcc atccgatcgt gcgcctcgag ctgccgcatc
     2341 tccccgatcg tcatctcgtt ggtcacgcgc gcgaacacgc gcagcggatc caggtccccg
     2401 acccgcacca gctcgatgat ggcgcgggag tagaggtagt cccccatgat gacggcgacc
     2461 tggtgggaga acagggcgtt cagcgtcggc ataccgcgcc gcagcgccga gtgatccacg
     2521 gagtcgtcgt ggacgagcgt ggcgagatga atcagctcga ccaccgccgc gagggtgacc
     2581 acccgggaat cgcccccgcc cgccgcctga tggctcagca ggagcagcgt cggccggaac
     2641 agcttcccct tcatccgcag gagatggtcg ttcgcgtcgg cgatgatcgg aaaatcggcg
     2701 gcgatgatgc gtcgcagctc cgccgccacg cggtcgagct ccccggcgac cggacgctgg
     2761 atgtccgaca acgacacccg gatggcctgt tcgctcacgc tagtgcgccc gggcgagcgc
     2821 tttcacgcgc gcccccacgt ccatgaaccg gatgtccacg gcgaacacac gcccgtacag
     2881 gtcacgcgct tcccccggct tcccctgctc ctcgagcgcc cgggcgagcc agtacaggat
     2941 cccgaggcgt tcctggtccc ccttggaagg cagctcgagc gcccgacgca ggatcgattc
     3001 tgcgacgccg aatgcgccct tgtcgtggaa acacacgccc agggcctccg acgtccgcag
     3061 cttcccctcc ggggcccgca acgccttctg gaactccgcg atcgcctcgt cgagcaggcc
     3121 catctccttg aacgccaccc cgagatcgta atgcgactga aaatcggttt cctcgatgtt
     3181 ctcgtcgatg
//
LOCUS       DBNGZW010000294         3034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1252697, whole genome shotgun sequence.
ACCESSION   DBNGZW010000294 DBNGZW010000000
VERSION     DBNGZW010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1252697"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..653
                     /locus_tag="AC16JC_07045"
     CDS             213..653
                     /locus_tag="AC16JC_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164490.1"
                     /translation="MTRTRLAGLAAALSVLAALGCRTRESPSTDARTAVAVPPEARDA
                     VLAEMRTMLGSLNGILVAATGSDSGAMRQAAAASGMAMAADPTLERYLPEQFLQLGTH
                     THLQFDSLAAAIGAAAPRDTAIARLARLTGNCVSCHAQYRLVLR"
     gene            complement(672..2204)
                     /locus_tag="AC16JC_07050"
     CDS             complement(672..2204)
                     /locus_tag="AC16JC_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164491.1"
                     /translation="MPGAHLVAQQPADPVIDTVIVVSHNVFDPPDGTPRVVARLGNAL
                     HVTTRPGVIRRLLLMGPGTPVDSARLIETERSLRELGVFRFVLLDTVRVDDRLALRVE
                     TGDGWSTSPQLNFASAAGSITWSAAMVERNFLGAATFVSLAYGHNPDRHSVDAQFASP
                     GLVLHHAPLLAAYSNLSDGRLGAWRYGRPFYRSDARWSLVTEGEAASHRVLEFQDGVL
                     SDSARRRTLALSLTGGVALYGTERGYVRLWASAGWRREDFAPESSTTSPYSGFGTLGV
                     GVDAGRVRYAVIEHLNGFGRREDVDLSSTLRLGLWAAPRAWGYPAGRAGVGPEVQGQV
                     GARWRGGLALLRGELHGVVGSAGLDSGLARGTLDVASLALRGHAMLLHLEGRALRRPK
                     FGEVFDPWSTQNGPRGFGAHAFTGTRLVYAMLEDRVVVADELWGLVGLGVAPSVEYGG
                     AWYAEREAARLGGDAGLALRLGSPRSSRGDVAELDVSWRWGDGFTGSPWALTFRKSFT
                     LK"
     gene            complement(2246..2803)
                     /locus_tag="AC16JC_07055"
     CDS             complement(2246..2803)
                     /locus_tag="AC16JC_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164492.1"
                     /translation="MTNLLDAFARRLCLTLLPWFSLSCDILPRKGDTLGEQQQAQWLI
                     AARLVVDTDPSLTLVRLRAAPLDSAHGGHHVLLARFEFNPSEGSGDEYALNIALDLGN
                     VRAREIGTPYPLGPAPAGIPAYGTVTCLCRPLKPDSMVGNLMLLHRGVGQLYGRIDAT
                     LFFTAWDDSSRHVSYRLRQRIDGMR"
BASE COUNT          486 a         1112 c          998 g          438 t
ORIGIN      
        1 ttgaaccgcc gaatggaggt tttgcagacc tctgccttac cacttggcta cggcgcccgg
       61 aggaggcata agctcgcagg ctgtttcgac tttctcaacc cctgcgcccc gaaatatgag
      121 gcggtcgtgc aaaaccctca ccggattcgt agcctcgtct tcatgacgac ccgcctaggg
      181 tgccctcacg cccatcgaaa ggaagcccgc caatgacccg gacaagactc gccggcctcg
      241 cggcagcgct cagcgtcctc gccgcgctgg ggtgtcgcac ccgtgaatcg ccgagtaccg
      301 atgcgcgcac ggcggtcgcc gtgccacccg aagcgcgcga cgccgtcctg gccgagatgc
      361 ggacgatgct cggctccctg aacggaattc tcgtcgcggc gaccggctcc gattcggggg
      421 ccatgcgcca ggcggccgcc gcgtcgggca tggcgatggc ggccgatccg acactcgaac
      481 ggtacctgcc ggagcaattc ttacagctcg gcacgcatac ccacttgcag ttcgacagcc
      541 tcgcggcggc cattggcgcc gccgccccgc gggacaccgc gatcgcgcgc ctcgcccgcc
      601 tcaccggcaa ctgcgtctca tgtcatgcgc agtaccgtct ggtgttgcgc tgaacggagc
      661 tacggagggt tctacttgag ggtgaagctc ttgcgaaagg tcaacgccca ggggctgccc
      721 gtgaacccgt ccccccagcg ccacgacacg tccagctccg ctacgtcgcc gcgcgacgac
      781 cgcggggacc cgagccgcag cgcgagcccg gcgtctcccc ccagccgtgc cgcctcgcgc
      841 tccgcatacc aggcgccgcc atactccacg gagggagcga caccgagacc gaccagtccc
      901 cacagttcgt ccgccaccac cacccgatcc tccagcatcg catacaccag tcgcgtgccg
      961 gtgaaggcgt gcgcgccgaa cccgcgcggc ccgttctggg tggaccacgg atcgaacact
     1021 tccccgaact tgggccgccg cagtgccctg ccctcgagat gcagcagcat ggcgtggccg
     1081 cgcagcgcga ggctcgcgac gtcgagcgtc ccccgggcga gcccggaatc gagtccggcc
     1141 gaccccacga cgccgtgcaa ttcgccccgc agcagtgcga gtcctccgcg ccagcgcgcc
     1201 cccacctgcc cctgcacctc gggccccacc ccggcgcgcc cggccgggta tccccacgcc
     1261 cgcggtgcgg cccacaggcc cagccgcagg gtggacgaca gatccacgtc ttcgcgtcgg
     1321 ccgaagccgt tcaggtgctc gatcacggcg taccgcacac ggcccgcgtc gacccccacg
     1381 cccagcgtgc cgaacccgga atacggggac gtggtcgacg actccggggc gaagtcctca
     1441 cggcgccacc cggcgctggc ccagagccgc acgtagccgc gctcggtgcc gtacagcgcc
     1501 acaccaccgg tgagcgacag cgcgagcgtg cgccgtcgcg cgctgtccga gagcacgccg
     1561 tcctggaact cgagcacccg gtggctcgcc gcttcccctt ccgtcacgag cgaccagcgc
     1621 gcgtcgctgc ggtagaaggg ccgtccgtag cgccacgccc ccagccggcc gtcggagagg
     1681 ttgctgtagg cggcgagcag cggcgcgtgg tgcagcacca gccccgggct cgcgaactgc
     1741 gcgtccaccg aatggcgatc ggggttgtgc ccgtaggcga gactcacgaa cgtcgccgcg
     1801 cccaggaagt tccgctccac catcgcggcg ctccaggtga tcgacccggc ggcgctcgcg
     1861 aagttgagct gcggcgaggt gctccatccg tctcccgtct cgacgcgcag cgccagccgg
     1921 tcgtccacgc gcaccgtgtc gagcagcacg aaacggaaca cccccagctc gcgcaagcta
     1981 cgttccgtct cgatcagccg ggcggaatcg accggcgtcc cgggccccat caacagcaag
     2041 cgccggatca cgcccgggcg ggtcgtaacg tgcagcgcgt tgccgagccg cgcgacgacc
     2101 cgcggcgtgc cgtcgggcgg atcgaacacg ttgtgcgaca ccacgatcac ggtatcgatc
     2161 accggatccg ccggttgttg ggcgaccaga tgcgcgcccg gcacgagtag tgccagcgcg
     2221 catgccagcc tccgtgctcc cgccctcacc gcatcccgtc gatgcgttgc cgcaaccggt
     2281 agctcacgtg gcggctcgag tcgtcccatg cggtgaagaa tagcgtcgcg tcgatgcgtc
     2341 catagagctg gccgacgccg cgatgcagga gcatgaggtt ccccaccatg gaatcgggtt
     2401 tgagcggccg gcacaggcag gtgacggtgc cgtaggcggg gatccccgcg ggcgccgggc
     2461 ccagcgggta cggcgtcccg atctcccggg cgcggacgtt tcccaggtcc agcgcgatgt
     2521 tgagcgcgta ttcgtcgccc gatccctcgc tcggattgaa ctcgaagcgt gccagcagta
     2581 cgtggtgccc gccgtgcgcg gaatcgagcg gcgccgcgcg cagccgcacg agggtgaggc
     2641 tcggatcggt gtcgacgacc agtcgggcgg cgatgagcca ctgggcctgt tgttgctcgc
     2701 ccagcgtgtc gcctttgcgg gggaggatgt cgcagctgag agaaaaccac ggcaacagcg
     2761 tcaagcagag acgtcgagcg aaggcgtcaa gcagattcgt caagcggact cgtcccgaca
     2821 cgcgcttttc cgtatgatgg ggaataggaa tgatgctgga ggacggtcgc ctgcagccca
     2881 tcgtacctgg tgagtcttcg cgccgatcca agtaagctaa tggtgcacca cgtcgccatc
     2941 gaagtcgcct ccgacgtcgt gcgcgtggct cagggcttcc gtggaccggg tgcgttctcc
     3001 cgtggtgagc agatggtgag ggcggcgctc tcgg
//
LOCUS       DBNGZW010000295         2436 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_638566, whole genome shotgun sequence.
ACCESSION   DBNGZW010000295 DBNGZW010000000
VERSION     DBNGZW010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2436)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_638566"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1922
                     /locus_tag="AC16JC_07060"
     CDS             <1..1922
                     /locus_tag="AC16JC_07060"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 94 protein"
                     /protein_id="HIR5164493.1"
                     /translation="TERDGETGAVLATNPYNQDFAGRVAFAHVSETARSASGDRLSFL
                     GRNGSLARPAALRQRELSGQFGAGLDPCAALQVEIALGPGESRRLVFILGQGRDRAHA
                     RELVRRHGSVDAADSALQAVQRFWSDTLETVQVRTPDDSFDLLMNRWLLYQVLSCRLW
                     ARTGFYQPGGAYGFRDQLQDVMPLALARPEVARAHLLRAAGRQFLEGDVQHWWHEPSG
                     RGLRSRCSDDLLWLPYAVAHYVDTTGDRDVLDASVHFLEAPILAAGETEAYGLPRVSA
                     TTASLFDHCVRAIDKGLTTGAHGLPLMGSGDWNDGMSRVGTQGRGESVWLGWFLHTVL
                     RQFIPLCEQRDPGRATRYAGEADRLAHMLERSWDGEWYRRGYYDDGTPLGSAQNDECK
                     IDSIAQSWAVLSGAAPRRRAEQAMDAVRTQLVRRGARVVLVLAPPFDRSAQDPGYIKG
                     YPPGVRENGGQYTHAAVWTVMAVARLGNGDEAVELFHLLNPINHTRSAADVERYKTEP
                     YVMAGDVYAHPAHAGRGGWTWYTGSAGWMYRAGLESILGLKRHGVSFELDPCIPAAWP
                     EYSITWRYGSSGYEILVANPDHRCRGIAQAELDGEAVDPAAIPLVDDGATHLVRVVLG
                     EQVRSASHAGAAART"
     gene            complement(1942..>2436)
                     /locus_tag="AC16JC_07065"
     CDS             complement(1942..>2436)
                     /locus_tag="AC16JC_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164494.1"
                     /translation="EAGEIVFDEDPPRPSSGVAPLGTVAGMPLVEVGVAGRKLRALFH
                     TGATLSCLREVDTRTLRCVGLARDCYPGQGEFTTVLRAVTLMLGDREVSLECGILPPA
                     IEQALRPVDVHGIVGTDLLRAFAVGWGPGFSELRLVALPFAAPVRLARFEVIHSSGER
                     RISP"
BASE COUNT          374 a          824 c          868 g          370 t
ORIGIN      
        1 tgacggagcg cgacggggag acgggcgccg tcctggccac gaacccctac aaccaggact
       61 ttgcgggtcg ggtcgccttt gcgcacgtca gcgagacggc gcgctccgcc tccggggatc
      121 ggctttcctt cctgggacgg aacggctcgc tggcgcggcc cgccgcgctc cgccagcgcg
      181 agctgtcggg tcagttcggt gccggactgg atccgtgcgc cgccctgcag gtcgagatcg
      241 ccctgggtcc gggggaaagt cgccgactcg ttttcatcct gggtcagggg cgggatcgtg
      301 cgcacgcccg tgagctggtg cgccgccatg gctcggtgga cgccgccgac agcgcattgc
      361 aggcggtgca gcgattctgg tccgacacgc tcgagaccgt ccaggtgcgc acgccggacg
      421 actcgttcga tctgctcatg aaccggtggc tcctgtacca ggtcctgagc tgccggctct
      481 gggccaggac cggcttctat cagccgggtg gtgcctatgg cttccgcgat cagctccagg
      541 acgtcatgcc actcgcgctg gcgcggcccg aggtggcgcg ggcgcacctc ctccgggccg
      601 ccggccggca gttcctggaa ggagacgtgc agcactggtg gcacgagccg tcgggccggg
      661 gattgcgcag tcgctgctcg gacgatctgc tctggctgcc gtacgccgtg gcgcattacg
      721 tggacacgac cggtgaccgc gacgtcctgg acgcgtccgt tcacttcctc gaggcgccga
      781 tcctcgccgc cggcgagacg gaggcgtacg gactgcctcg tgtctccgcc actacggcgt
      841 cactgttcga ccattgtgtg cgcgccatcg acaagggcct gaccacgggc gcccacgggc
      901 tgccgctgat gggcagcggt gactggaacg acggcatgag ccgggtcggg acccagggcc
      961 gcggcgagag cgtctggctc ggatggttcc tgcacacggt gctgcggcag ttcatcccgc
     1021 tgtgcgagca gcgggacccg ggacgagcga cgcgatacgc cggcgaggcc gatcggctgg
     1081 cgcacatgct cgagcgttcc tgggacgggg agtggtaccg gcgcggatat tacgacgacg
     1141 ggaccccgct cggctcggcc cagaacgacg agtgcaagat cgactcgatt gcccagtcct
     1201 gggctgtcct ctccggagca gcgccccgga ggcgcgccga gcaggcaatg gacgccgtcc
     1261 gcacgcagct ggtgcggcgc ggcgcacggg tcgtcctcgt actcgcgccc ccgttcgatc
     1321 ggtcggctca ggatcccggc tacatcaagg gatacccgcc aggggtgcgg gaaaatgggg
     1381 ggcaatacac ccatgcggcg gtgtggaccg tgatggccgt cgcccggctc ggaaacggcg
     1441 acgaggccgt ggagctgttt cacctcctga acccgatcaa ccatacgcgg agcgccgcgg
     1501 acgtggaacg gtacaaaacc gagccgtacg tcatggccgg agacgtttac gcccacccgg
     1561 cccacgccgg gcgtggggga tggacgtggt ataccggctc ggcaggctgg atgtatcgcg
     1621 cggggctgga gagcatcctc ggactcaagc gccacggcgt gagcttcgag ctggatccct
     1681 gcatccccgc ggcgtggccc gagtactcca tcacgtggcg ctacgggagc agcggctacg
     1741 agatcctcgt cgccaatccg gatcaccgct gccggggaat cgcgcaggcg gagttggacg
     1801 gggaggcggt ggatccggcc gccattcccc tggtggacga tggagcgacg cacctggtgc
     1861 gcgtggtgct gggggagcag gtgcgcagtg cctcgcacgc gggggcggcg gcgcgcacat
     1921 gagcgaatgc aggcttgccg actacggtga aatgcgtcgt tcgcctgatg agtgaatcac
     1981 ttcgaatcgg gcgaggcgaa cgggggctgc aaagggcagc gccaccaggc gcaactcgct
     2041 gaaaccgggt ccccatccca cggcgaacgc ccgcaacagg tccgtgccca cgatgccgtg
     2101 cacatccacc ggccgcagcg cctgctcgat ggccggcggc agtatcccac actcgaggct
     2161 gacctcccgg tctccgagca tcagcgtgac cgctcgcagc actgttgtga attccccctg
     2221 tcccgggtag cagtcccggg ccagtccgac acaacgcagc gtcctggtgt cgacctcgcg
     2281 gaggcacgac agcgtggcgc ccgtgtgaaa cagcgcccgc agcttcctgc cggccacccc
     2341 gacctcgacc aacggcatcc ccgcgacggt tcccagtggg gcaacgcccg agctggggcg
     2401 cggagggtct tcgtcaaaga cgatctcgcc ggcctc
//
LOCUS       DBNGZW010000296         4708 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_26777, whole genome shotgun sequence.
ACCESSION   DBNGZW010000296 DBNGZW010000000
VERSION     DBNGZW010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_26777"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..506)
                     /locus_tag="AC16JC_07070"
     CDS             complement(30..506)
                     /locus_tag="AC16JC_07070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164495.1"
                     /translation="MRDLTRRLSLAVLLALLLSAPAAAQSLRPSLGPQLGFASDNFDL
                     FVGGQLSYQVAPRFDLYPSVIIYFPDGPLNAWGLNFNVRYWPRLSTPNPGLYAGGGLG
                     YTHVSAFGVGDSRVGLNLLGGWEFHTPGLQPFVELRAILASDVNRVEFAGGLNFKL"
     gene            complement(555..1052)
                     /locus_tag="AC16JC_07075"
     CDS             complement(555..1052)
                     /locus_tag="AC16JC_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164496.1"
                     /translation="MITTPKTLALVVAGLALAGAAQAQQRTASGGSYTPIDPIRIGGH
                     AGYNFDVEQLALGAQATLPLTRMFEIYPSFDYYFVDVGTLWALNADLKIRPPTAQGAL
                     YVGGGMNYLHFSDAGFSNGDVNLNLLGGFELRRQPLHPYAEARLILGNGSAFQLVGGV
                     SFNLH"
     gene            complement(1181..2095)
                     /locus_tag="AC16JC_07080"
     CDS             complement(1181..2095)
                     /locus_tag="AC16JC_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164497.1"
                     /translation="MRTFPLALSGLTAVALIGTACSDSSTALNTDQTQVRAAYEGLTG
                     YTDDDFGDTGAVGTSAVSPAFDSYGVQDTAIAPPFWGRVRVVRGGPRPVVSLNIVVQG
                     DSAWLTRGVGFQGIFLADTSAEGTFNPTSKPLVEGVQQRAVFVRDAAALHGWRAVALT
                     LLDWQPTAADRRTVQVERVAVYRNGTLQFAVANPHSLIAVDTNVPQLSQGDTVRVEAH
                     VANTTGGSFSPATFAFLHVRHADPTGIRWRRIRMVDNGNGDFSLEWIVRFTGRDRFVV
                     DALDAATLQLGTADNYRANEWGIPFRVE"
     gene            complement(2222..2731)
                     /locus_tag="AC16JC_07085"
     CDS             complement(2222..2731)
                     /locus_tag="AC16JC_07085"
                     /inference="COORDINATES: protein motif:HMM:NF024183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR5164498.1"
                     /translation="MVELILGVLLAAGGLFFVLRPIFAPPPAGALETGGTPAWGDPED
                     DLSPRTVALRALKEIEFDRATGKLADGDYESLKGKYTAEALQALRQETPAKPQAETGR
                     RAAGTPPARASTARACPTHGPRPESDAEFCSECGRRLAAAPGFCPRCGTTLPPDARFC
                     ARCGGRVAA"
     gene            complement(2737..3327)
                     /locus_tag="AC16JC_07090"
     CDS             complement(2737..3327)
                     /locus_tag="AC16JC_07090"
                     /inference="COORDINATES: protein motif:HMM:NF015853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HIR5164499.1"
                     /translation="MSALDRRKFLSVLAVGLVSAHRSIAQQPGQDSLPTAGPSGKLWD
                     PNRAGPSMQPVTAEDNDAAIQAIEKQLKCTCGCGLDVYTCRTTDFTWPVSPKMHRRVI
                     EMSKAGLSGPGIIAQFVSENGLEILMAPPKRGFNLAGYFVPGALILAVGAVLLVVMRR
                     WVRSAPRSPALAAEAPPEASPAELERLRDALQHTDV"
     gene            complement(3324..>4708)
                     /locus_tag="AC16JC_07095"
     CDS             complement(3324..>4708)
                     /locus_tag="AC16JC_07095"
                     /inference="COORDINATES: protein motif:HMM:NF027651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HIR5164500.1"
                     /translation="LSVGICLGMWWAYVELGWGGYWAWDPVENASFLPWLTMTAFLHS
                     VMIQEKRGMLKKWNLALIIGSWLLSIFGTFITRSGVIASVHSFTQSSVGYFFGAFLVI
                     SAVASFWLYARRLPLLQAESTLESLVSREASFLFNNLLLVGIAFSVLWGTLFPIISEA
                     VTGTKITVKAPFFNQVNIPLGLALLAFTGIGPLIAWRRSSLPNLRRQFAVPGAACVFA
                     LLVMLVAGMRDFYPLMAITIGAFVFVTIVQEFVRGVSARHRQYQEAWPLAFGRLIARN
                     RRRYGGYIVHVGIVVLFIAFAGMAFKTEEQATLRPGESATLKSPYGWTYTFTHLGVSQ
                     FDALNRQVTAATVEVSRNGKRVGMMTTEKRQHVDAEGNPTFQPSTEVGIRSDLREDLY
                     IVLAGVVNGTEQAVYRFTINPLVWWVWYGGMIVALGGLIVMWPGGGPVSVRREQAGYT
                     VKLVAGEKPS"
BASE COUNT          830 a         1573 c         1626 g          679 t
ORIGIN      
        1 gagcggggcc cagaagggca cgtccgtcgc tacagcttga agttcaggcc gcccgcgaat
       61 tcgacgcggt tcacatcact ggccagtatc gcgcggagct cgacgaacgg ctggaggccg
      121 ggagtatgga actcccatcc gcccagcagg ttcaggccca ccctcgaatc gcctacaccg
      181 aaggcgctga cgtgggtgta gccgagaccg ccgcccgcgt acagcccggg attgggcgtg
      241 ctgagtcgcg gccaatacct cacattgaaa ttcagccccc acgcgttgag cggaccgtcg
      301 gggaagtaga tgatcacgga cgggtagagg tcgaaccgcg gagcgacctg gtagctgagc
      361 tgacctccca caaagaggtc gaagttgtcc gacgcgaacc cgagctgagg gccgagagaa
      421 ggccggaggc tttgcgccgc ggctggggcg gacaggagca gcgccaacag gacagccagc
      481 gacaaccgtc gggtcagatc tctcacgctc agactccttg tgcggaagtg gtacggcacc
      541 gtgctacggc tcggttagtg caggttgaag ctcacgccgc cgaccagctg gaacgcgctg
      601 ccgttcccca ggatcagccg cgcttcggcg tacggatgca gcggctgccg acggagctcg
      661 aacccgccca gcagattgag attcacgtcg ccgttgctga acccggcgtc gctgaaatgc
      721 aggtagttca tcccgccccc gacgtacagc gccccctggg cggtcggcgg gcggatcttg
      781 agatccgcgt tgagggccca gagcgtcccg acatccacga agtagtagtc gaacgacgga
      841 tagatctcga acatccgggt cagcggcagg gtggcctgcg cgccgagagc cagctgctcg
      901 acgtcgaagt tgtagccggc gtggccgccg atgcggatcg gatcgatggg agtatagctg
      961 ccgccgctcg cggtgcgctg ctgtgcctgc gcggcgccgg ccagcgccag gccggccacg
     1021 acgagcgcga gtgtcttggg cgtcgtgatc atgatcgctc ctcttgcagt gggaggaggg
     1081 tgttgggtcg cgaacaatta gtgaaggcgg acctggaaaa gcaagggcgg cgggcacgca
     1141 tcgcgtgtcc gccgcccact ttccgtcgca gatcgagcgg tcattcgacc cggaacggga
     1201 taccccactc gttggcacga taattgtctg cggtgccgag ctgcaacgtg gccgcgtcga
     1261 gcgcgtccac cacaaaccga tcacgacccg tgaagcgcac gatccattcg agtgagaagt
     1321 ccccgttgcc gttatcgacc atccggatgc ggcgccagcg aatgccggtg ggatccgcgt
     1381 gccgcacgtg caggaacgcg aacgtggcag gagagaacga tccgccggtc gtgttcgcga
     1441 cgtgcgcctc gacccggacc gtgtcgccct gggacagctg cggcacgttc gtgtcgaccg
     1501 cgatcagcga atgggggttg gcgaccgcga actggagggt gccgttgcgg tagaccgcca
     1561 cgcgctcgac ctgcacggtc cgccggtcgg cggccgtggg ctgccaatcg aggagcgtga
     1621 gcgccaccgc gcgccatccg tgcaaggccg ccgcatcgcg cacgaacacg gcccgctgct
     1681 gcacaccctc gacgagcggc ttgctcgtcg gattgaacgt gccttccgcc gacgtgtcgg
     1741 ccaggaagat gccctggaag ccgacgccgc gcgtcagcca ggcggaatcg ccctgcacta
     1801 cgatgttcag gctcaccacc gggcgcgggc cgccgcgcac cacgcggacg cggccccaga
     1861 atggcggcgc gatcgcggta tcctgcacgc cgtaactgtc gaacgcgggc gacacggccg
     1921 aggtgcccac ggcgcccgtg tcgccgaaat cgtcgtcggt gtatccggtg agcccctcgt
     1981 aggcggcccg cacctgggtc tgatcggtgt tgagcgcggt cgagctgtcg ctgcaggccg
     2041 tcccgatcaa cgcgacggcg gttaaccccg agagggcgag agggaacgtg cgcatcatgc
     2101 ggctcctgtg cgatgtgagt gcggctgctg cccgagaact gcagcaacag acgccggcca
     2161 ccggcgcgcg ttaaccgcat gaggtgcagc agatgccggt ggtgacgggc gtcaccaccg
     2221 ttcaggccgc gacgcgacct ccgcagcgcg cgcagaagcg cgcgtcgggc ggcagcgtcg
     2281 tgccgcagcg ggggcagaag cccggagccg cggccagccg gcgcccgcac tccgagcaga
     2341 actccgcgtc ggactccggg cgagggccgt gcgtggggca ggcccgagcg gtcgacgcgc
     2401 gtgccggcgg agtgccggcg gcgcggcgcc cggtctccgc ctggggcttc gcgggcgtct
     2461 cctgccgcaa cgcctggagc gcttcggccg tgtacttccc cttcagcgac tcgtagtcgc
     2521 catccgccag cttgccggtg gcgcgatcga actcgatctc cttcagcgcc cgcagggcga
     2581 cggtccgcgg cgagagatcg tcttccggat ctccccaagc gggcgtcccg cccgtctcga
     2641 gtgcgcccgc aggcggcgga gcgaagatcg ggcggagcac gaagaacaga ccgcccgccg
     2701 ccaggagaac gcccaggatc agctcgacca tggggttcat acgtcggtgt gctgcagggc
     2761 gtcgcgcagc cgctcgagct cggcggggct cgcctcaggg ggcgcttcgg cggcgagcgc
     2821 cgggctccgg ggtgcgctgc gcacccagcg acgcatgacc accaggagca ccgcgccgac
     2881 ggcgaggatg agcgccccgg gcacgaagta gcccgccagg ttgaagccgc gcttgggcgg
     2941 cgccatcagg atctcaagcc cgttttcgct cacgaattgc gcgatgatcc ccggccccga
     3001 caatcccgcc ttgctcatct cgatgacgcg gcggtgcatc ttggggctca ccggccaggt
     3061 gaagtccgtc gtgcggcagg tatacacgtc gaggccgcag ccgcaggtgc acttcagctg
     3121 cttttcgatc gcctggatgg cggcgtcgtt gtcctcggcc gtaacgggtt gcatcgaggg
     3181 accggcacga ttggggtccc acagcttccc ggatggaccg gcggtgggca gcgagtcctg
     3241 cccgggttgc tgcgcaatgg atcgatgggc ggagacgagc ccgacggcca gcacgctcag
     3301 gaatttccgg cgatcgagcg cgctcacgag ggcttctccc cggccacgag cttgacggta
     3361 tatcccgcct gctcccggcg caccgatacc gggccacccc ccggccacat gacgatgagg
     3421 ccgcccagcg cgacgatcat cccgccgtac cacacccacc agaccagggg attgatggtg
     3481 aagcggtaca cggcctgctc cgtcccgttc acgacacccg cgaggacgat gtagagatcc
     3541 tcgcgcagat cgctccggat gccgacctcg gtggacggct ggaaggtcgg attcccttcg
     3601 gcgtccacgt gctgccgttt ctcggtggtc atcatgccga cgcgcttgcc gttgcgcgac
     3661 acttcgaccg tcgccgcggt cacctggcgg ttcagggcgt cgaactgcga gacccccagg
     3721 tgagtgaagg tataggtcca gccgtagggg ctcttcaggg tcgccgactc acccggccgg
     3781 agcgtggcct gctcttcggt cttgaacgcc atgccggcaa acgcgatgaa caacaccacg
     3841 ataccgacgt gcacgatgta gccgccgtag cggcggcggt tgcgcgcgat cagacggccg
     3901 aaggccagcg gccacgcctc ctggtactga cggtggcggg cggagacgcc gcgcacgaac
     3961 tcctgcacga tcgtgacgaa gacgaaggcg ccgatggtga tcgccatgag cgggtagaag
     4021 tcgcgcatgc cggcgaccag catgaccagc agggcgaaca cgcatgccgc cccgggcacc
     4081 gcgaattgcc ggcgcagatt cggcagcgag gagcggcgcc aggcgatcag cggaccgatg
     4141 ccggtgaacg cgagcagggc gagccccagc gggatgttga cctgattgaa gaacggcgcc
     4201 ttgaccgtga tcttcgtgcc cgtcaccgct tcggagatga tgggaaacag cgtcccccac
     4261 agcaccgaga aggcgatgcc gacgagcagc aggttgttga agaggaagga ggcctcgcgg
     4321 ctcacgagcg actcgagcgt cgactccgcc tgcagcagcg gcaaccgccg cgcgtagagc
     4381 cagaagctcg ccacggcgga gatcaccagg aaggcgccga agaaataccc gacgctcgac
     4441 tgcgtgaagg aatgaaccga cgcgatcacg ccggagcgcg tgatgaaggt gccgaagatc
     4501 gacagcagcc aggacccgat gatgagcgcg agattccact tcttcagcat cccccgcttc
     4561 tcctgaatca tcacggagtg caggaacgcc gtcatcgtga gccaggggag gaacgaggcg
     4621 ttctccaccg ggtcccacgc ccagtagccg ccccacccga gctccacgta ggcccaccac
     4681 atgccgaggc agatccccac cgacagga
//
LOCUS       DBNGZW010000297         2379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1118490, whole genome shotgun sequence.
ACCESSION   DBNGZW010000297 DBNGZW010000000
VERSION     DBNGZW010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1118490"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="AC16JC_07100"
     CDS             complement(<1..708)
                     /locus_tag="AC16JC_07100"
                     /inference="COORDINATES: protein motif:HMM:NF024676.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5164501.1"
                     /translation="MTKTERWLNLIAFLLDHHYPVAREELLGQVDDYADDWKSGDDTR
                     RESARRKFERDKKELRDLGVVLETRPVGVEHTEQPVEGYLLRPRDFYLPYIDVRTRPL
                     RAGAKAYGLAAVTLEPEEVPVLRRAAERVARLGDTTLGAAAKSALRKLAFDRPELEVR
                     EGERMLGAPAPEGFDREFSTLLAALRARHAVACRYYSIGRDAETERVIEPYGIMLLWG
                     RWYCVGRARERNALRVFR"
     gene            1047..1604
                     /locus_tag="AC16JC_07105"
     CDS             1047..1604
                     /locus_tag="AC16JC_07105"
                     /inference="COORDINATES: protein motif:HMM:NF017496.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HIR5164502.1"
                     /translation="MPQIAHAWTPDEVRALPDDGRRYEVVAGELLVTPAPSFHHQDAV
                     LRLVLALRDYAERTGVGYALMSPADIEAEEGALVQPDVFVGPLPEGRRPESWADFSHL
                     LLAVEVLSPATARADRTVKRLLFQRAGVPEYWIVDLDARLVERWRPRDERPEVITELL
                     PWQPDPTKVALEIDLPAFFAQVLGD"
     gene            complement(1637..>2379)
                     /locus_tag="AC16JC_07110"
     CDS             complement(1637..>2379)
                     /locus_tag="AC16JC_07110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020710164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HIR5164503.1"
                     /translation="IHLIGELRQAKPDLYVNLTTGTWPSPFWLRHADSIWRGGEDHSF
                     AGTGSWRQQWITYRDAATYAGIVQKGPLFPLSSLMLHGLIFAKSAEHLSDDPGGDFDA
                     ELRSYFGTGTQLQEMYISHALLSDANWDVLAECASWSRRNASVLRDTHWVGGDPAQLE
                     PYGWAAWTQERAILTLRNPSDQPRTLSLDPGTAFELPDGAPRTFSARSPWRRDMGKMA
                     IRVAAGAGHDFALAPFEVLTLEMTPLGD"
BASE COUNT          354 a          810 c          831 g          384 t
ORIGIN      
        1 gcggaacacc cgcagcgcgt tgcgctcgcg cgcccgtccg acgcaatacc atcggcccca
       61 caacagcata atcccgtacg gctcgatgac gcgctccgtc tcggcgtcgc gtccgatcga
      121 gtagtaacgg cacgccaccg cgtggcgggc acgcagcgcc gcgagcaagg tcgagaactc
      181 gcggtcgaac ccttccggcg ccggggcgcc gagcatgcgc tcgccttcgc ggacctcgag
      241 ctccgggcgg tcgaaggcca gcttgcgcag cgccgacttg gccgcggcgc ccagcgtcgt
      301 gtcgcccagc cgggcgaccc gctcggcggc acggcgcagc accgggactt cttccggctc
      361 gagcgtcacc gcggcgaggc cgtacgcctt cgcgccggcg cgcagcgggc gggtgcgcac
      421 gtcgatgtag gggagataga aatcgcgggg gcggagcagg taaccctcga ccggctgctc
      481 ggtgtgctcg acgccgacgg gccgcgtctc cagcactacg cccaggtcgc gcagctcctt
      541 cttgtcgcgc tcgaacttgc ggcgtgccga ctcgcggcgc gtgtcgtcgc ccgacttcca
      601 gtcgtcggcg tagtcgtcca cctggccgag cagctcctcc cgggccaccg ggtagtggtg
      661 gtccaggagg aaggcgatga ggttgagcca gcgctcggtt ttggtcatcg gggcgaagcc
      721 aacttagggc tggtggggga tcgcgcgaaa gggtgaagcg gtggtcggtg gtcggtgtgc
      781 agtggccggt ggcgctcgaa tgggttgagt caaccatagg ggagcggtgt gacaggtggg
      841 gcgggggaga cgcgttcgcg tgtcacttca cgtgacagtt tagccgagtc gcggccttgg
      901 ccgcgagaac attcgagggc tcaccctctg gcccgtcata attcgctccc cctcgcaacg
      961 aaatgatccc acatcacccg cggggctcgc ctagcgtcgt gcgtgtccac ttgccaaact
     1021 ctcgccgagc cgagcagact atgcgtatgc cgcagatcgc tcacgcctgg acgccggacg
     1081 aggtgcgcgc ccttccggac gacggccgcc gctacgaagt ggtcgccggt gagctgctcg
     1141 tgaccccggc gccgagcttc catcaccagg acgccgttct tcgactggtg cttgctctga
     1201 gggactacgc cgaacgcacc ggcgtgggct acgcgctcat gtcgccggcg gatatcgagg
     1261 cagaggaagg ggcgctggtc cagcccgatg tgttcgtcgg cccgctcccc gaaggccggc
     1321 gtccggagag ctgggccgat ttcagtcatt tgctcctcgc cgtcgaggtg ctctcacccg
     1381 cgaccgcccg cgccgaccgc accgtcaagc ggctgctgtt ccagcgtgcc ggcgtgccgg
     1441 aatactggat cgtggatctg gatgcacggc tggtggagcg ctggcgtccc agggacgagc
     1501 gtccggaggt gatcacggag cttttgccct ggcaaccgga tcccacgaag gtagcattgg
     1561 aaatcgacct gccggcgttc ttcgcgcaag tactcggcga ctgagggggt cagggggaga
     1621 ggtacccgga cagccttcag tcgccgagcg gcgtcatctc gagcgtgagc acctcgaacg
     1681 gcgcgagcgc gaaatcgtgc ccggcgccgg ccgcgactcg gatcgccatc ttccccatgt
     1741 cccgccgcca cgggctccgc gccgagaacg tgcgcggcgc accgtccgga agctcgaacg
     1801 ccgtacccgg atcgagcgac agggtcctgg gctggtcgct gggattgcgc agcgtgagga
     1861 tcgcccgctc ctgtgtccac gcggcccagc cgtacggctc gagctgcgcc ggatccccac
     1921 ccacccagtg cgtgtcgcgc aggaccgacg cattgcgccg gctccagctc gcgcattccg
     1981 ccagcacgtc ccagttcgcg tcgctcagca gcgcgtggct gatgtacatc tcctggagct
     2041 gggtgccggt gccgaagtag ctccgcagct ccgcgtcgaa gtccccgccc gggtcgtcgc
     2101 tcaggtgctc ggcgctcttc gcgaagatca gcccgtgcag catgagcgag ctgagcggga
     2161 agagcggccc cttctgcacg atcccggcgt acgtggcggc gtcgcgatag gtgatccact
     2221 gctgccgcca gcttccggtg ccggcgaaac tgtggtcctc gccgccacgc cagatcgaat
     2281 cggcgtggcg cagccagaac ggcgacggcc aggtgccggt ggtgaggttc acatagaggt
     2341 cgggcttcgc ctggcgcagc tctccgatca ggtggatca
//
LOCUS       DBNGZW010000298         2434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_639319, whole genome shotgun sequence.
ACCESSION   DBNGZW010000298 DBNGZW010000000
VERSION     DBNGZW010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_639319"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="AC16JC_07115"
     CDS             complement(<1..320)
                     /locus_tag="AC16JC_07115"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR5164504.1"
                     /translation="MAEPPVRILSRYLVRQHFAPLAFALSALTALMLLNQIAKQIGNL
                     VGKGLPPGIIVEVFVLSVPFIVAMTLPMAVLVAVLHTMSRLVADSEVTAMTANGISVG
                     RIARP"
     gene            351..>2434
                     /locus_tag="AC16JC_07120"
     CDS             351..>2434
                     /locus_tag="AC16JC_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164505.1"
                     /translation="MRPPASLVFFLLTLLAPPARAQGPGADTARAPAFRLAYRVERLG
                     PVRVAALAPGGRLGARLPPALAAAAWETRVRAALPAGGLALAGAAADTGIGLSALARL
                     APGAQEPVEPRVFEAAGAPGVFGQFASLGMDLSLRFELKADQFRNLRCNAVERQQAIS
                     GCRAGFPAITPNPQYAIRSGGVVGQRLHVNVDFDSQREFDANNNLQVWYEGLEDEILR
                     RVEAGNVSFQIPASRFISAAIPANNFGVQAVSQIGPLEVRGIYAQQKGNVVRDRTYNI
                     GETTTEPIDRQFRDLDYEQGRFFFAIDPAAVPGFPALDILNIVPAALPDSLRVASLQV
                     YRVRAPNAGTNQNIGGIHAVACGPGARAVDCSIERAGPFDWELLVQGRDYYVDQSRTW
                     IALAGRLDPNDFLAVSYIPEGQSACGPASRCVGTFPVARNPDTTAVDTLRLVYDPRPG
                     VTAASPSFRFEIRAAYRVGGQDLARSSVALDLAVNQRQRPDGSSDTYLEILGLALAND
                     FTKLDQYNRLFPRDRDPGQGAPLRDYYVVFPHLQPFADTAKLVATERNDSLYRTPRLL
                     LAQQGPPSVFTLTLHGDASATSDRGTLSLASFQIREGSEKLYLGNRLLQRDQDYTIDY
                     TVGLVTFKNPDSLFTGSQAVVRAQFEERASFAVAPTSIYGMAATYDLGQLGQVHLTGL
                     FQKEQTIFTRPQL"
BASE COUNT          361 a          890 c          804 g          379 t
ORIGIN      
        1 ggccgcgcga tgcggccgac gctgatgccg ttcgccgtca tggccgtgac ctcgctgtcg
       61 gcgaccagcc ggctcatcgt gtgcagcacg gccaccagca cggccatcgg cagcgtcatc
      121 gccacgatga acggcacgct cagcacgaac acttccacga tgatgcccgg cggcagtccc
      181 ttcccgacga gattgccgat ctgcttggca atctggttga gcagcatcag cgcggtgagc
      241 gccgacagcg cgaaggcgag cggtgcgaag tgttgccgca ccaggtagcg ggagaggatg
      301 cgcacggggg gttcggccat tatattcgga actccaagcg gctacaaggg atgcggcctc
      361 ccgcctcgct cgtctttttc ctcctcacgc tgctcgctcc tccggctcgg gcgcagggcc
      421 ccggcgccga caccgcgcgc gcgcccgcgt tccggttggc gtatcgggtc gagcgtctcg
      481 ggcccgtgcg cgtggcggcg ttggcgcccg gcggacgcct cggggcgcgg ctgccgcccg
      541 ccctcgcggc cgccgcgtgg gagacccgag tccgcgccgc actgcccgcc ggcgggctcg
      601 cgctggccgg cgcggccgcg gacaccggga tcgggctctc cgcgctggcg cgtctcgcgc
      661 cgggggccca ggaacccgtc gagccccgcg tgttcgaggc cgcgggcgcc cccggcgttt
      721 tcgggcagtt cgcctcgctg gggatggatc tgagcctgcg cttcgagctg aaggccgacc
      781 agttccgcaa cctgcgctgc aacgcggtgg agcgtcagca ggccatctcg gggtgtcgcg
      841 ccggattccc cgccattacc cccaatccgc agtatgcgat ccgctccggc ggcgtggtcg
      901 ggcaacggct ccacgtcaac gtggacttcg atagccagcg cgagttcgac gccaacaaca
      961 atctgcaggt gtggtacgaa ggactcgagg acgagatcct gcggcgcgtc gaagccggca
     1021 acgtgtcctt ccagatcccc gcatcccgct tcatcagcgc ggccattccg gcgaacaatt
     1081 tcggcgtgca ggcggtgtcc cagattggcc cgctggaggt ccgcggcatt tacgcgcaac
     1141 agaagggcaa cgtggtccgg gaccggacct acaacatcgg ggagaccacc accgagccga
     1201 tcgaccgtca gttccgcgac ctcgattacg agcaggggcg cttcttcttc gcgatcgacc
     1261 cggcggccgt gccgggattt cccgcgctcg acattctcaa catcgttccc gcggcgctcc
     1321 ccgactcgct gcgcgtagcc agcctgcagg tgtatcgcgt ccgggcgccc aacgccggca
     1381 ccaaccagaa catcggcggc atccacgccg tcgcctgtgg tccgggggcc cgcgcggttg
     1441 actgctcgat cgagcgggcc ggcccgttcg attgggagct gctggtgcag gggagagact
     1501 actacgtgga ccagagcagg acatggatcg ccctcgccgg ccggctcgat cccaacgact
     1561 tcctggcggt ctcctacatc ccggaggggc agtccgcctg tggtccggcg agccgctgcg
     1621 tgggcacttt cccggtggcg cgcaatccgg acacgaccgc ggtggacacg ctgcgcctcg
     1681 tctacgaccc gcgccccggg gtcaccgcgg cgagtccttc gttccgcttc gagatccgcg
     1741 ccgcgtaccg ggtgggcggg caggacctgg cgcggagtag cgtggcgctc gatctcgccg
     1801 tcaatcagcg gcaacgcccc gacggctcca gcgacaccta cctcgaaatc ctgggcctgg
     1861 cactcgccaa cgacttcacc aagctcgatc agtacaaccg gctcttcccg cgcgaccgcg
     1921 atcccgggca gggagcgccg ctgcgggact actacgtcgt cttcccgcac ctgcagccgt
     1981 tcgccgacac ggcgaagctg gtcgccaccg agcgcaacga ctcgctgtat cgcacgccgc
     2041 gcctcctgct cgcccagcag gggccgccct cggtgttcac gctcacgctg cacggcgacg
     2101 cctcggcgac ttccgaccgc ggcaccctgt cgctcgcgag cttccagatc cgcgagggca
     2161 gtgagaagct ctacctcggc aaccggctgc tgcagcgcga ccaggactac accatcgact
     2221 acacggtcgg cctggtcacg ttcaagaacc cggactcgct cttcaccggg agtcaggcgg
     2281 tcgtgcgcgc ccagttcgaa gagcgcgcgt cgttcgcggt cgcgcccacg tcgatctacg
     2341 gcatggcggc cacctacgac ctgggtcagc tgggccaggt gcacctgacg gggctgttcc
     2401 agaaggagca gaccatcttc acccgcccgc agct
//
LOCUS       DBNGZW010000299         2701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1662221, whole genome shotgun sequence.
ACCESSION   DBNGZW010000299 DBNGZW010000000
VERSION     DBNGZW010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1662221"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(463..539)
                     /locus_tag="AC16JC_07125"
     tRNA            complement(463..539)
                     /locus_tag="AC16JC_07125"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(503..505),aa:Pro,seq:tgg)
     gene            816..2180
                     /locus_tag="AC16JC_07130"
     CDS             816..2180
                     /locus_tag="AC16JC_07130"
                     /inference="COORDINATES: protein motif:HMM:NF051835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005085 - guanyl-nucleotide exchange
                     factor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HIR5164506.1"
                     /translation="MRRFVTVLSTVALAGSLVASNVAAATPTREARANSLLAIQPAPA
                     VTAITASGGTGNDGHTCALTSAGTVLCWGSNAFGQLGDGSTVTRTIPVEVRGLSGPIV
                     AIAAGGLHTCALTSTGGIECWGINVRGQLGDGTGIDSASPVGVSGLASGVSAITSGAG
                     QVCALTSAGAVKCWGMNVAGRRTDTGDSLAPLDIPDLGNGVKAIAAGLSHTCAVTSGG
                     GVKCWGDGSWGQLGDGKTAEQGIPSSETPVNVAGLDSGVVAIAAGDAHTCALTVAGAV
                     KCWGANLANGAEDNSGVPIDVPGLDGDITSIATTYWHTCALTSLGAVECWGSNDWGQL
                     GDGTTHSSRVPVKVRTAAGNIAVAAGGYHTCVVTSTRQVKCWGANGSGQLGDGTTTDR
                     LTPVDVAGQTARLSTGLPATGTTPEVREAGDQPWAQVVLVGLVALLAFLLRPHRRPSP
                     PVRG"
     gene            complement(2283..>2701)
                     /locus_tag="AC16JC_07135"
     CDS             complement(2283..>2701)
                     /locus_tag="AC16JC_07135"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HIR5164507.1"
                     /translation="LALGVGTGRPPVPVGVSICADIEYPEVARLLALRGARLLAVPSA
                     DMEPFRAQQAANLASRAIENNVYVALANTVDRRRGVTFFGGSGIAGPDGSLVSAGYLR
                     PRLVTATLSDAAVTTSGGPGAYLRTRRPDTYRGLLA"
BASE COUNT          449 a          894 c          969 g          389 t
ORIGIN      
        1 accgctgatg ggcgtctcgc cggaccccca gcggacgaag atcgcttccg atgggctggc
       61 gtagaggtag ccctggctgg ccgtggatcg cgaccgcggc gacgtcgcgg gcgccgatca
      121 cgaacaagcg gacaggctcg ccgccacggc gatgatcgcg atggcgatgg accctcggcg
      181 cgtcggaacc cgcatttcgg ctgcctccca atcgcgtacg cggcagccgt gctgcataga
      241 caggcctgac cgaaccggca ggacctgggc gtttcagccg gcggtatgtg gcgagtcggg
      301 attgtcccgg gggcgcgtga tcgatgggaa gtgccggaaa gcccgaagtg cgacggggaa
      361 cgggagtcgt cgctgcggga gcgacccttg gcaggggagt ctcgcaaacc ccagtcgccg
      421 tcgccgtcgg cggcggcgtc gtcgtcgtcg tcgtcgacgg gatggtcggg gaggcgggat
      481 tcgaacccgc gatctcgtgc tcccaaagca cgtgcgttgc caggctacgc tactccccgc
      541 tgcgctcaaa gaacacacag gccgcttgag cgatacgcca agggtagcgc tcgcccacca
      601 agtacggagg cgcgaccgta cacctgtcat cgcggcacgg cgcagccgcg tttcactacc
      661 gatgacgaac gggctgaacg gcactggtga tgcgcagacc cggagcggat tctgatcagt
      721 cacgacaggc gccgacgccc ggttggcagt ggccgcccag cccgaatctc ggaccggccg
      781 aggcgaacgc gcgtaaggcg cccaaggagg gtggcatgcg ccgcttcgtg acagtcctct
      841 cgacagtcgc gctcgctggc tcgctcgtcg catcgaacgt cgcggccgct actcctaccc
      901 gagaggcgcg ggcaaatagc cttctcgcca tccagccggc gccggcggtc acagcgatca
      961 cggcgagcgg agggactggc aacgacggcc acacgtgtgc cctcacgtct gccgggacgg
     1021 ttctgtgctg gggcagcaac gcattcggac aactcggtga cggttccacg gtcaccagga
     1081 cgattccggt cgaagtgcgc gggctcagcg gcccaatcgt agccatcgcg gccggcggtc
     1141 ttcacacctg cgcgctcacc tccaccggcg gtatcgagtg ctggggcatc aacgtccgag
     1201 gccagctcgg cgacggcacg ggtatcgaca gcgcgtcccc agtcggcgtg tcggggcttg
     1261 cgagcggagt cagcgccatc acgtcagggg caggccaggt ttgcgcgctc acctcggccg
     1321 gtgccgtgaa atgctggggg atgaacgtgg cgggcagacg cacagacact ggcgacagtc
     1381 tcgcgccgct cgacatccca gacctcggaa atggggtcaa ggcaatcgcc gccgggctca
     1441 gccacacgtg cgccgtcacg tcgggcggag gggtcaagtg ctggggtgac ggctcttggg
     1501 gtcagctggg cgatggcaag accgccgagc agggcatccc gagcagtgag acgccggtca
     1561 acgttgccgg gctcgatagc ggcgtcgtcg cgatcgccgc aggcgatgcg cacacctgcg
     1621 cgctgacggt ggcaggcgcg gtcaagtgtt ggggtgcgaa ccttgcgaac ggcgcagagg
     1681 acaacagtgg cgttccgatc gacgtgccgg ggctcgatgg ggacatcacg agcatcgcga
     1741 cgacttactg gcacacgtgc gccctgacgt cactcggcgc agtggagtgc tgggggagta
     1801 atgattgggg gcagctcggg gatgggacga cacactcaag ccgagtccca gtgaaggtgc
     1861 ggaccgccgc gggcaatatc gcggtcgctg ccggcggcta tcacacgtgc gtagtcacct
     1921 cgacccgtca ggtcaagtgc tggggagcga atggctccgg ccagctcggc gacggcacga
     1981 cgaccgatcg attgacccct gtggacgtgg ccggtcagac ggcacgattg tcgacgggcc
     2041 tgccagccac gggaacgact ccggaggtgc gcgaagccgg cgaccagccg tgggcgcagg
     2101 tggtgctcgt cggccttgtc gcgctgctag cgttcctcct gagaccgcat cgccgacctt
     2161 ccccaccggt ccgaggctga ctgtaggggg ctcacacgct cgatcgcact gtggcatgga
     2221 ccgggctctt gcccgctgtg ctcacggaaa ccggcgcggc cgcggtgtcc cgatcgaggg
     2281 atctacgcca gcaacccgcg atacgtgtcc gggcgcctcg ttcgcagata ggcgcccggc
     2341 ccacccgacg tcgtcaccgc cgcgtcgctc agcgtggcgg tgacaaggcg cggccgaagg
     2401 tagccggccg acaccaggct gccatccgga ccggcgatcc cgctgccgcc gaagaaggtc
     2461 acgccgcgcc gacggtcgac ggtgttggca agcgcgacgt agacgttgtt ctcgatcgcc
     2521 cgcgaggcca ggttggccgc ctgctgcgcc cggaacggct ccatgtcggc tgacggcacg
     2581 gccaggagcc tggcgccgcg cagggccagc agccgggcca cctccgggta ctcgatgtcg
     2641 gcacagatcg acacgccgac gggaaccggc ggtcgcccgg tcccgacgcc cagggcaagg
     2701 t
//
LOCUS       DBNGZW010000300         2446 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_164694, whole genome shotgun sequence.
ACCESSION   DBNGZW010000300 DBNGZW010000000
VERSION     DBNGZW010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2446)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_164694"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..186
                     /locus_tag="AC16JC_07140"
     CDS             <1..186
                     /locus_tag="AC16JC_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018348045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBM96 family carbohydrate-binding protein"
                     /protein_id="HIR5164508.1"
                     /translation="FVVGTGGAERYGFDAPKPNSEVREGDTWGVLKLTLMPDAYRWQF
                     VPVAGKTFSDSGSGRCH"
     gene            complement(188..631)
                     /locus_tag="AC16JC_07145"
     CDS             complement(188..631)
                     /locus_tag="AC16JC_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-like domain-containing protein"
                     /protein_id="HIR5164509.1"
                     /translation="MPGRSLVTVLILLLAAGAGPARAQRRDSLPTAIATVFRTAYPAA
                     KILNVSRERRDGKVVYEIESQDGPTRRDLIYDLAGRNLETEEVIPADSVPAEVRAAVQ
                     RDVAGGTIVNAERVTRETVILYEVGVRRNGRTRYLTYDLAGKRVE"
     gene            811..1665
                     /locus_tag="AC16JC_07150"
     CDS             811..1665
                     /locus_tag="AC16JC_07150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164510.1"
                     /translation="MRRSRYGVLSVVPLVFVALPWGRRLQAQATAPYLRDRGPGVPMS
                     MLGSYVEKGHVLVYPFFEWYADRNLEYKPRELGYGTSGDDYRGRFRASEGIFYVGYGI
                     THDLAVEMEAAVITAELRPSLSDTSALKPARVHEAGLGDVEGQIRWRPQRETESRPEI
                     WTFFETVLPLQRTRHIIGTQDWEFSAGFGVTKGFHWGTMTFRASAEYTDRQVDAGEYA
                     VEYLRRLSPSWQIIGAVEGTQLDEISVITEAQWFFSRYARLRLNNAWGITPNATDIAP
                     EVGIMFSF"
     gene            complement(1675..1860)
                     /locus_tag="AC16JC_07155"
     CDS             complement(1675..1860)
                     /locus_tag="AC16JC_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164511.1"
                     /translation="MSWKKLLLDALKTALVAFVVLQLKEYYDAHQFDTAGTAADGTLI
                     GVGMFLVGVVQKVVKKS"
     gene            complement(1872..>2446)
                     /locus_tag="AC16JC_07160"
     CDS             complement(1872..>2446)
                     /locus_tag="AC16JC_07160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164512.1"
                     /translation="LSSDMGGGQLTVSAPAGSRWRMSGSAGYYDYRIRSLTAANSGDT
                     RSNRLAPGGLAYLSDFNLLDLLLAVDYSGIGERYPLRLVGDYVHNAGADDLNSGWGAD
                     VFVGRAAAQGDLRYRYGYGVTDADAVLAAFSHDNTTLGTNYEIHTLSLDAVPLGGLFL
                     NATLYHYRPHVAVPGRKYQNRLRLNAMVTF"
BASE COUNT          400 a          822 c          816 g          408 t
ORIGIN      
        1 ttcgtggtgg ggacgggggg cgcggagcga tacggattcg acgcgcccaa gccgaacagc
       61 gaggtgcgcg agggcgacac ctggggcgtc ctgaagctga cgttgatgcc ggacgcctac
      121 cggtggcagt tcgttcccgt cgcgggcaag accttcagcg attccggcag cggccgctgc
      181 cactgagcta ctcgacgcgc ttccccgcca gatcgtaggt cagatagcgc gtcctgccgt
      241 tgcgccggac ccccacttcg tacaggatca ccgtctcgcg cgtcacccgt tcggcgttga
      301 cgatcgtgcc gcccgcgacg tctcgctgca cggccgcgcg cacttccgcc ggcaccgagt
      361 cggcggggat gacctcttcg gtctcgagat tgcggcccgc cagatcgtag atcaagtcgc
      421 gtcgtgtcgg gccgtcctgg ctctcgatct catagacgac cttcccgtcc cggcgctccc
      481 ggctcacgtt caggatcttg gcggcggggt aggccgtgcg aaacacggtc gcgatcgccg
      541 tggggaggct gtcccggcgt tgggcccggg cgggtccggc gccggccgcg aggagcagga
      601 tgagaacggt gaccagcgag cgaccgggca cgacgacctc caggcgtgaa gggttcgaca
      661 gaaccgccag caagttccat gcgggagagg gccccccctt aaagatcggt cgccctgttg
      721 tcggggcgcc acactgcacc gttcgcgagg acgaagcgcg attgttatcg tagcgcgacc
      781 ccgatatcgc cccccggtca ggaggtcgac atgcgtcgca gccgttacgg agtcctgagc
      841 gtcgtgccgc tcgtcttcgt cgcactcccg tggggccgcc ggctccaggc ccaggcgacc
      901 gctccctacc tgcgcgacag gggtcccggt gtcccgatgt ccatgctcgg cagctatgtc
      961 gagaagggcc acgtgctcgt gtatccgttc ttcgagtggt acgccgaccg caacctcgag
     1021 tacaagccga gggagctcgg ctacgggacc tccggagacg actaccgcgg ccggttccgc
     1081 gcctccgagg ggatcttcta tgtcggttac gggatcacgc acgatctcgc ggtcgagatg
     1141 gaagccgccg tcatcacggc cgagctgcgg ccttccctga gcgatacctc cgccctcaag
     1201 cccgccagag tacacgaggc cggactgggc gatgtggagg ggcagatccg gtggcgcccg
     1261 cagcgcgaaa cggagtcgcg ccccgaaatc tggacctttt tcgaaaccgt cctgccgctg
     1321 cagcgcacgc ggcacatcat cggcacccag gactgggagt tcagcgccgg gttcggggtg
     1381 acgaagggct ttcactgggg cacgatgacg tttcgggcca gcgccgagta cacggatcgc
     1441 caggtggacg ccggcgagta tgcggtcgag tacctgcgcc ggctctcgcc gtcgtggcag
     1501 atcatcgggg cggtcgaagg gactcagctg gacgagatct cggtcatcac cgaggcacag
     1561 tggttcttca gtcgctacgc ccgcctcagg ctcaacaacg cctgggggat cacgccgaac
     1621 gcgaccgaca tcgcgcccga ggtcggcatc atgttctcgt tctgaggtag ccggctagga
     1681 cttcttcacg accttctgca cgacccccac cagaaacatc ccgacgccga tcaatgtgcc
     1741 gtcggccgca gtgcctgcgg tgtcgaactg gtgggcgtcg tagtactcct tgagctggag
     1801 gaccacgaag gcgactagcg ccgtcttcag cgcgtcgagc agcagcttct tccagctcat
     1861 cggagcctcg ctcagaaggt gaccatcgcg ttcagccgta gcctattctg atacttccgg
     1921 cccgggaccg caacatgcgg ccggtagtgg tacagcgtgg cgttcaggaa caatccgccg
     1981 agcgggacag cgtccagcga cagcgtgtgg atctcgtagt tcgtccccaa ggtcgtattg
     2041 tcatgagaga acgcggccag caccgcgtcg gcatcggtca ccccgtaccc gtagcgatag
     2101 cgcaggtcgc cctgggcggc ggcccggccg acgaagacgt ccgctcccca gccgctattg
     2161 aggtcgtccg cccccgcgtt gtggacgtaa tcgcccacca ggcgcagcgg gtagcgctcg
     2221 ccgattcccg agtaatcgac ggcaagcagc aggtcgagca ggttgaagtc ggagagatac
     2281 gccaggccgc ccggggccag ccggttgctg cgcgtgtcgc cgctgttggc cgccgtgaga
     2341 ctgcggatgc ggtagtcgta atagcccgcc gaccccgaca tcctccaccg cgaacccgcc
     2401 ggcgcgctga cggtgagctg gccgcccccc atgtcgctgc tgagat
//
LOCUS       DBNGZW010000301         1891 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1596822, whole genome shotgun sequence.
ACCESSION   DBNGZW010000301 DBNGZW010000000
VERSION     DBNGZW010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1891)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1891)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1891
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1596822"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..300)
                     /locus_tag="AC16JC_07165"
     CDS             complement(<1..300)
                     /locus_tag="AC16JC_07165"
                     /inference="COORDINATES: protein motif:HMM:NF015639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HIR5164513.1"
                     /translation="MSHPELPCYPCPYASSCCAYGVTLSDEEAARISEVHGADVLYRT
                     RWGEWRTRVRRGRCVFLRDNVCTIHAQDYYPAVCRGFPWTDAETGGPYEYDRTICP"
     gene            complement(297..>1891)
                     /locus_tag="AC16JC_07170"
     CDS             complement(297..>1891)
                     /locus_tag="AC16JC_07170"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR5164514.1"
                     /translation="NVGLRHWIGGVALACSSLTAPAPAVAQATGGSGQPAALVVLLVV
                     DQMIPDYFDRFGAQLTGGLGRLRRDGAFFTHGMQDHAITQTAPGHSTLLSGRPPSSTG
                     IITNDDGVGDARYPLIDAPGEPGASPRNFNGTALYDWMLARDSGTRVLAVSRKDRGAI
                     LPVGRARGDVYWFAGGRFTTSRYYADALPAWLRAFNDRHAVARLAGKRWELLLPPADY
                     PEPDSMPWERRGRDIGFPHVLPAAAAIDTGVLLYPWMDSLTLDLALEGVRQTGVGRRA
                     HPDLLVVSLSTTDKVGHEWGPDSREMHDHILRLDRWLGRFLDSLGTMVPARRTFFVLA
                     ADHGTQAVPEYNREVRHRPGGRQWLGSYVTEGERRFDARYRRSFGFRFEYGLLLADVD
                     ALHALGVGVDSLAEAWAADARRRPGVARVYTPKSLAAAPGSDRFAARWRRAIPPGTGW
                     LLAAVPSEGVIWSKTGVANHGTALDVDVRVPIIFAGPGIRPGRYERPVATEDIGPTLA
                     ALIGVRPTEPVAGHPIPEVVGR"
BASE COUNT          316 a          668 c          635 g          272 t
ORIGIN      
        1 gggacagatc gtccgatcat actcgtaggg accgccggtt tcggcatcgg tccaggggaa
       61 tccgcggcag accgccgggt agtagtcctg agcgtgtatg gtgcagacgt tgtccctgag
      121 gaagacgcaa cgcccgcgcc tcacccgtgt tctccattcc ccccagcgcg tccgatagag
      181 tacgtccgct ccatggactt cgctgatgcg agccgcctct tcgtcgctca gcgtgacgcc
      241 gtacgcgcag cacgatgagg cgtacggaca cggatagcag ggcagctcgg gatggctcat
      301 cgtccgacga cctcgggaat ggggtgaccg gcgaccggct ctgtcggccg cactccgatg
      361 agggcggcca aggtcggacc gatgtcctcc gtggcgaccg gccgttcgta ccgtccgggc
      421 cggatgcccg gcccggcgaa gatgatcggc acccggacat cgacatcgag cgcggtgccg
      481 tggttggcga cgccggtctt cgaccagatc accccttccg acggcacggc agcgagaagc
      541 cagccggtgc ccggtggaat cgcccggcgc cagcgggccg cgaatcgatc gctccccggc
      601 gcggccgcga gcgacttcgg agtgtagacc cgggcgacgc ccggcctccg tcgcgcatcc
      661 gctgcccacg cttcggccag actgtccacc cccacaccaa gcgcgtggag cgcatcgacg
      721 tcggccagca gcagtccgta ttcgaaccgg aagccgaagc ttcggcgata tcgggcgtcg
      781 aagcgtctct caccctcggt gacgtaggaa ccgagccact gtcgcccgcc ggggcgatgc
      841 cgcacttccc gattgtactc gggcactgcc tgcgtgccgt gatccgccgc cagcacgaag
      901 aaggtccgcc gggccggcac catggtgccg agggaatcga ggaagcgccc gagccagcga
      961 tccagccgga ggatgtgatc gtgcatctcg cgcgagtccg gcccccactc gtgaccgacc
     1021 ttgtcggtgg tggacaggga gacgaccagg agatcggggt gcgcccgccg tccgaccccc
     1081 gtctgccgta ccccctcgag cgcgaggtcc agcgtcagcg agtccatcca cgggtaaaga
     1141 agcacaccag tatcgatcgc cgcggccgcg ggcagcacgt gcggaaagcc gatgtcacgc
     1201 ccccgccgct cccacggcat cgagtcgggc tccggatagt cggcgggcgg aagcaggagc
     1261 tcccaccgct tccccgcgag gcgcgccacc gcgtgccggt cgttgaaggc gcggagccag
     1321 gccggaaggg cgtcggcgta atatcgactg gtcgtgaaac gcccgcccgc aaaccagtag
     1381 acatcgccgc gcgcgcggcc gaccggcagg atggcacccc gatcctttcg cgataccgcc
     1441 agcacgcgag tacccgagtc gcgcgccagc atccagtcgt agagcgcggt gccgttgaag
     1501 ttccggggcg aggcaccggg ttcgccggga gcatcgatga gcgggtatcg cgcgtcaccc
     1561 acgccatcgt cgttggtgat gatccccgtg ctcgacggcg gtcgtcccga caggagcgtc
     1621 gaatgacccg gcgcggtctg ggtgatcgca tgatcctgca tgccgtgcgt gaagaacgcg
     1681 ccgtcccgac gcagtcgtcc caggccgccg gtcagctgcg ctccaaacct gtcgaagtag
     1741 tcgggaatca tctggtcgac gaccagcagc acgaccagcg ccgccggctg gccgcttcca
     1801 cccgtcgcct gcgccactgc cggtgccgga gcggtcagcg atgagcacgc gagtgcaacg
     1861 ccgccgatcc agtgccgcag tccgacattc a
//
LOCUS       DBNGZW010000302         2563 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_369046, whole genome shotgun sequence.
ACCESSION   DBNGZW010000302 DBNGZW010000000
VERSION     DBNGZW010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_369046"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="AC16JC_07175"
     CDS             <1..243
                     /locus_tag="AC16JC_07175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164515.1"
                     /translation="TVADTLLGSLGRPPSALATRAETNDPEAHALFRQGQVLFNLRTA
                     RTLNQAIALFERAAARDPTYARAEASLAMALAVLPA"
     gene            268..1038
                     /locus_tag="AC16JC_07180"
     CDS             268..1038
                     /locus_tag="AC16JC_07180"
                     /inference="COORDINATES: protein motif:HMM:NF024579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5164516.1"
                     /translation="MTRAVAAAERAIAIDSTIAESYTALGYGYSLLGELSRAEESFRR
                     AVALDSTVATAWGWYGLLANRLGDFRAAHERIGRARRLEPASLIARQWEAQVYLLERN
                     FPAADSEASATMALDSTFMLAWQQRENVLLALGKVTQAVDLLERHVAALPAGRPEEAH
                     GMLAYAYARAGRGREARAVLETMRAQSGGRIPPTGATAAALEELGDHEAAVALLGEAI
                     GRHDVWLVQFPMAPRYDRLRKDPRAAAMLARLGTRRHD"
     gene            1031..>2563
                     /locus_tag="AC16JC_07185"
     CDS             1031..>2563
                     /locus_tag="AC16JC_07185"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5164517.1"
                     /translation="MTDGMLGLATALADRYRIERELGAGGMATVYLAHDLKHDRKVAV
                     KVLRPELAAVIGAERFLSEIKTTANLQHPHILPLFDSGAADTFLFYVMPFIEGESLRD
                     RLTREKQLPIGDAVRIATEVAGALDYAHRHNVIHRDIKPENVLLHDGRALVADFGIAL
                     AASKAGGTRMTETGMSLGTPTYMSPEQAMGEREITARSDVYALGCVLYELLTGDPPFT
                     GSTAQAIVARVLTETPRPITAQRHTIPPHVEAAVLTALEKLPADRFASAAEFAAALAS
                     PSYTRSMAGSRAAASSAAQWWRRAALGAATALVVCAALAVWALRRSGNAPATPVRHWN
                     IVLPDSAPLDFFAPSLYGEGQPALAIARDGTRIAYVVRYGGTTSLYYRQLDEAVSHPL
                     AGTEGASQPFFSPDGEWIGFFAGGELKKVAVAGGTPLSITRAEAPRGATWTTDGRVIF
                     SDFATPGISFVSASGGNRSQLPGPKGSSAGPAEGRPTLSWPSLLPGEQWVVGSSNERR
                     LVL"
BASE COUNT          390 a          897 c          882 g          394 t
ORIGIN      
        1 accgtggcgg acaccctgct cgggtcgttg ggccgtccgc cgtctgcttt ggcgacgaga
       61 gcggaaacca atgatcccga agcccacgcc ctgttccggc aggggcaggt gctgttcaac
      121 ctgcgcacgg cccggaccct gaaccaggcg attgccctgt tcgagcgcgc cgcggcgcgg
      181 gatccgacgt acgcccgcgc ggaagcctcg ttggcgatgg ccctggccgt gctccccgcg
      241 taagtgcagg acagcacaac accgatcgtg acccgagccg tcgccgcggc cgagcgcgcc
      301 atcgcgatag actcgaccat cgccgaatcg tacactgccc tgggctacgg ttactccctg
      361 ctgggcgagc tgagccgggc tgaggagagc ttccgccgcg ccgtggcgct cgactccacg
      421 gtggccacgg cgtggggctg gtacggacta ctcgccaatc ggctgggcga cttccgcgct
      481 gcgcacgagc ggattggacg ggcgcgccgg ctcgagcctg cttcgctgat cgccagacag
      541 tgggaagccc aggtctatct gttggagcgc aacttcccag ccgcggattc ggaggccagt
      601 gccaccatgg cgctcgattc gacgttcatg ctggcgtggc aacaacggga gaacgtcctg
      661 ctggccctgg ggaaggtcac gcaggccgtg gacctgctcg agcgccatgt cgcggcgctt
      721 cccgcaggtc gcccggagga ggcccacggc atgctcgcgt acgcctatgc ccgagcgggc
      781 cggggcaggg aggcgcgcgc cgtgctcgaa accatgcggg cgcaatcggg cggccggatt
      841 ccgcccacgg gagccaccgc cgcggcactc gaggagctgg gcgaccacga ggcggcggtg
      901 gcgctcctcg gcgaggcgat cggccggcat gacgtctggc tggtgcagtt tccgatggcc
      961 ccgcgatacg acaggctgcg gaaggacccg cgggccgctg cgatgctggc caggctcggg
     1021 acgaggcgac atgactgacg gcatgctggg ccttgccact gccctggccg accgctaccg
     1081 catcgagcgc gagctcggcg caggcggcat ggccaccgtc tacctcgccc acgacctgaa
     1141 gcacgaccgc aaggtggcgg tgaaagtgct gcgtcccgag ctcgcggccg tgatcggcgc
     1201 cgagcggttc ttgagtgaga tcaagaccac cgcgaacctc cagcacccgc acatcctccc
     1261 gctgttcgat tccggcgcgg ccgacacttt cctcttctat gtcatgccct tcatcgaggg
     1321 cgagtcgctg cgcgaccggc tgacccgcga gaagcagctc cccatcggcg acgcggtgcg
     1381 catcgccacc gaagtcgctg gcgccctcga ctacgcccac cgccacaacg tcatccaccg
     1441 cgatatcaag ccggagaacg tcctgctgca cgatggccgc gccctggtcg ctgatttcgg
     1501 catcgcgctg gcggcgagca aggcgggcgg cacgcggatg acggagacag ggatgtcgct
     1561 cggcaccccg acctacatga gtcccgagca ggcgatggga gagcgggaga ttaccgcccg
     1621 gagcgatgtg tatgcgctgg ggtgcgtgct gtacgagctg ctcacagggg acccgccgtt
     1681 caccggctct accgcgcagg ccatcgtggc gcgggtgctc accgaaacgc cgcggccgat
     1741 cacggcccag cgccatacca tcccacccca cgtggaagcg gcggtgctga ccgcgctcga
     1801 gaaactcccg gccgaccggt ttgcctcggc cgcggagttc gccgcggcgc tagcgagtcc
     1861 gagttatacg cggtcgatgg ccgggtctcg agcagcggcg tcatcggctg cccagtggtg
     1921 gcgccgcgcg gccctgggtg ccgcgaccgc cttggtggtg tgcgcggcgc tcgcggtgtg
     1981 ggcgctgcgc cgttcaggca acgcacccgc gacgccggtc cgtcattgga acatcgtgct
     2041 gcccgattcc gcaccgctcg atttcttcgc ccctagcctc tacggcgaag gacagcccgc
     2101 tctcgccatt gcccgggacg gcacccggat cgcctatgtc gtccgatacg gcggaacaac
     2161 cagtctgtat taccgccaac tggatgaggc cgtctcccac ccactcgcgg gcaccgaggg
     2221 cgcgtcccag ccattcttct cgccggacgg cgagtggatc gggttctttg ccggcggaga
     2281 gttgaagaag gtggcggtcg ccggcggtac cccgttgagc atcacgcgag ccgaggcgcc
     2341 gcgaggcgcg acctggacca ccgatggccg ggtcattttc tccgacttcg ccaccccggg
     2401 catctccttc gtgtccgctt cgggcgggaa ccggtcacaa ctgccggggc cgaaggggag
     2461 ttccgccgga ccagcagagg gccgccctac gctctcctgg ccctcgctgc ttcccgggga
     2521 gcaatgggtg gtaggatcgt cgaatgaacg acgcctggtg ctg
//
LOCUS       DBNGZW010000303         6489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_369066, whole genome shotgun sequence.
ACCESSION   DBNGZW010000303 DBNGZW010000000
VERSION     DBNGZW010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6489
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_369066"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3810
                     /locus_tag="AC16JC_07190"
     CDS             <1..3810
                     /locus_tag="AC16JC_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="beta strand repeat-containing protein"
                     /protein_id="HIR5164518.1"
                     /translation="SDPFDILIGSVTRIVFTQQPTTDTAGVSITPAVQITAQDGSGNT
                     VTGFTGTVTVSIVVNPGGGTLSGTKSLAAVAGVATFPGLSIDKAATGYRLQAAAGTLK
                     DTSQAFSILAGTATKLAFTQQPTNATAGLSISPSVKVAGQDAFGNTSTAFTGDVTVAI
                     GTNPSGGQLSGTLTLAAVSGVATFSNLSIDKAGTGYTLKATATGLTTATSTSFNITTG
                     AATQLAFTVQPVTTAAGATIPTVKVTARDASGNTVTAYTGNVTMAIGNDPGNGTLSGT
                     VVVTAVQGVATFSNLSINRSGTGYTLKASATGLTTAFSTPFDILPGAAAVLAFGPQPV
                     STTATTTLAPIKVTAQDAQGNTATGCTGNVTVAIAVNPGGGTLSGTKTVAAVAGVATF
                     STLSIDKAGTGYRLAATASGLTPATSAAFSITAGPAANLVFTVQPTTTEATATITPAV
                     KVAAQDVAGNTVTSFTGSVTIAIGTNPNSGILSGTKTVTAVAGVATYSDLSIDKLGTG
                     YTLRATSTGLTAATSTAFNIVAGTATQLAFTTQPVTTAAGATIPTVKVTARDGSGNTV
                     TTYTGNVTMAIGTDPGNGTLSGTLTVTAVQGVATFSNLSINRSGTGYTLKASATGLTT
                     AFSTPFDILPGAAAVLAFGPQPVSTTATTTLAPIKVTAQDALGNAATGFTGNVTVAIA
                     VNPGGGTLSGTKTVAAVAGVATFSTLSIDKVGTGYRLAATATGLTPDTSSAFSITAGT
                     AATLTFTVQPTQAGAGAIITPAVKVAALDAGGNTATGFAGNVTVAMGTNPSGGILSGT
                     KTVAAVAGVATFSDLSIDKLGTGYTLTAAATGLTSATSATFNIVVGTATQLAFTTQPT
                     NTTAGATMATVRVTARDAQGNTATGYTGNVTVAIGTNPGGGTLSGTKTVAAVAGVATF
                     SSLTIDKSGTGYTLTAAANNLAGATSSTFNITAAALSQLGFLVEPVNTVAGATITPAV
                     KVAGQDAFGNTVTSFTGSVTIAITPGSGTAGATLSGTKTVNASAGVATFSTLSIDKTG
                     ADYHLTASSGVLTPATSAIFAITAGTATHLTFEQQPTSTVAGVAIAPAVTVSARDALG
                     NPVKTFAGSVTVAIAAGTGAAGATLSGTKVVTASGPDGLATFSTLSINKAATGYRLVA
                     TATGLTADTSTAFTITAGAATKLAFTVQPVAALAGAVITPAVEVTAQDALGNTVTGFT
                     GSVAIAITSGTGTVGAVLGGTTPVVAVAGVATFSDLSINLAGTGYKLTATTSGLTSAT
                     SGAFPIN"
     gene            3918..5063
                     /locus_tag="AC16JC_07195"
     CDS             3918..5063
                     /locus_tag="AC16JC_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR5164519.1"
                     /translation="MPTATEPTAPPARGLEGIVANESSICYVYGEEGRLIYRGYDIND
                     LADHSTFEETAYLLLNGDLPTRDRLKDFAAQLKASQKLDKVVQRIIRDAPPNANPMDV
                     LRTAVSASVFVDPDRDNNGREAEYRKAVRLIAQLPTMVAMFHRLRNGEKPLSPRRGLS
                     LAANFLYLVSGKKPEREAEHAFDVALILHADHELNASTFAARVIAATLADVHGAVTGA
                     IAALAGPLHGGANTEVMKMLLEIQSPERAEAWIQNALAQRHKIMGFGHRVYRTEDPRA
                     AQLRRMAEAQGARAGQLQWAAIQRTIEDVVRREKGLYCNVDFYSASLYYVMGIPLDLY
                     TPIFAVSRVSGWCAHVLEQHADNRLIRPRAEYMGHMNRRWVAVRKRG"
     gene            5066..6271
                     /gene="icd"
                     /locus_tag="AC16JC_07200"
     CDS             5066..6271
                     /gene="icd"
                     /locus_tag="AC16JC_07200"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013706269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HIR5164520.1"
                     /translation="MSGTKIDYRDGRWVVPEHPIIPFIEGDGTGPDIWRASRRVFDAA
                     VRQTSGGRRQVEWHEVLAGEKAFKATGNWLPDETLEVIRAHRVAIKGPLTTPIGGGIR
                     SLNVALRQVLDLYACVRPVRWFKGVPAPVKDPGKLDIVIYRENVEDVYAGVEFPAESR
                     ESNELIALLERLGKKVRPGSAIGIKPMSKFGSQRLVAKAIRFALESGRTSVTLVHKGN
                     IMKFTEGAFRDWGYETAKTQFGDRTIAEADMGKGTGDAGRVVIKDRIADSMFQQLLLR
                     PAEYSILATPNLNGDYLSDACAAQVGGLGLAPGGNVGDGYAVFEATHGTAPKYAGLDK
                     INPGSVILSGAMMFAELGWVEVSRAIITGIEGAIGAGRVTYDLARQMPGATEVSTSTF
                     ADAIIEHLA"
     gene            6268..>6489
                     /locus_tag="AC16JC_07205"
     CDS             6268..>6489
                     /locus_tag="AC16JC_07205"
                     /inference="COORDINATES: protein motif:HMM:NF014808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family peptidase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5164521.1"
                     /translation="MNSTLTAQPFPEIDTPLLAVAVAAATTLPAALAPLDQATGGALG
                     RALANGDFKGKRDETLLAYGSGKARRVLLV"
BASE COUNT         1133 a         2240 c         2270 g          846 t
ORIGIN      
        1 agcgatccgt tcgacatcct gatcggctcg gtcacccgga tcgtattcac ccagcaaccg
       61 acgaccgata cggcgggagt gagtatcacc cccgccgtgc agatcacggc gcaggatggg
      121 tcggggaaca cggtgacggg tttcacgggc accgtgacgg tctcgatcgt ggtgaacccc
      181 ggcggcggaa cgctctcggg gacgaagagt ctcgcggcgg tcgccggggt cgccacgttc
      241 ccgggattga gcatcgacaa ggcagccacc ggctaccggc tgcaagccgc cgccgggacc
      301 ctgaaggaca cgagccaggc cttcagcatc ctggccggga ccgccacgaa gctcgccttc
      361 acgcagcagc cgaccaatgc gacggccggc ctgtcgatca gtccgtcggt gaaggtcgcg
      421 gggcaggacg cattcgggaa cacgtccacc gctttcacgg gtgacgtgac ggtggcgatc
      481 ggcacgaatc ccagcggcgg gcagctgtcc ggcaccctca cgctggccgc ggtcagcggg
      541 gtcgccacgt tctccaacct gagcatcgac aaagcgggga cgggctacac gctcaaggcc
      601 acagcgacgg ggctgacgac ggcgacgagc acgtcgttca acatcactac gggggcggcc
      661 acgcagctgg cctttaccgt gcaaccggtg acgaccgcgg cgggcgcaac gatcccgacc
      721 gtcaaggtga cggcacgcga cgcctccggc aacacggtga cggcatacac cggcaacgtg
      781 acgatggcga tcggcaacga cccgggcaac gggacgctgt ccggcaccgt ggtcgtgacc
      841 gcggtgcagg gtgtcgccac gttctcgaac ctcagcatca accggagcgg gaccgggtac
      901 accctcaagg cctccgccac cggtctgacg acggcgttca gcacaccgtt cgacatcctc
      961 cccggcgcgg cagcggtgct cgcgttcggc ccgcagccgg tgagcacgac ggcgaccacg
     1021 acgctcgcgc cgatcaaggt cacggcgcag gacgcccagg ggaacaccgc aaccggctgc
     1081 acgggcaacg tgacggtggc gatcgcggtg aaccccggcg gggggacgct gtcgggcacg
     1141 aagaccgtgg cggcggtggc cggcgtggcg acgttcagca cgctgagcat cgacaaggcg
     1201 gggacgggct accggctggc ggcgaccgcg agcggcctga ccccggctac gagcgcggct
     1261 ttcagcatca cggccgggcc ggccgcgaac ctcgtgttca ccgtgcagcc gaccactacc
     1321 gaggcgacgg cgaccatcac gccggcggtg aaggtcgcgg cacaggatgt cgcggggaat
     1381 acggtgacga gcttcaccgg cagcgtgacc atcgccatcg ggaccaaccc caacagcggg
     1441 atcctgtcag gcaccaagac cgtgaccgcc gtggcgggcg tggcgacgta cagcgatttg
     1501 agcatcgaca agctggggac gggctacacg ctgcgggcga cctccaccgg gctcacggcg
     1561 gccacgagca cggcgttcaa catcgtagcc ggtacggcga ctcagctcgc gttcacgacg
     1621 cagcccgtga ccaccgcggc gggtgcgacg atcccgaccg tcaaggtgac ggcacgcgac
     1681 gggtcgggca acacggtgac gacgtacacc ggcaacgtga cgatggcgat cgggaccgac
     1741 cccggcaacg ggacactgtc cggcaccctg acagtgactg cggtgcaggg cgtcgccacg
     1801 ttctcgaacc tcagcatcaa ccggagcggg accgggtaca ccctcaaggc ctccgccacc
     1861 ggtctgacga cggcgttcag cacaccgttc gatatcctcc ccggcgcggc agcggtgctc
     1921 gcgttcggcc cgcagccggt gagcacgacg gcgacgacga cgctcgcgcc gatcaaggtc
     1981 acggcacagg atgccctggg gaacgccgca accggcttca cgggcaacgt gacggtggcg
     2041 atcgcggtga accccggcgg gggaacgctg tccggcacga agaccgtggc ggcggtggcc
     2101 ggggtggcga cgttcagcac gctgagcatc gacaaggtcg ggacgggcta ccggctggcg
     2161 gcgaccgcga ccggcctgac cccggatacc agctcggcct tcagcatcac ggccgggacg
     2221 gccgcgaccc tcacgttcac cgtgcagccg acccaggccg gagcgggggc gatcatcaca
     2281 ccggccgtga aggtcgccgc gctggacgcc ggggggaaca cggcgacggg cttcgccggc
     2341 aacgtgacgg tcgccatggg gaccaatccc agcggcggaa tcctgtcggg caccaagacc
     2401 gtagccgccg tcgcgggcgt ggcgacgttc agcgacttga gcatcgacaa gctggggacg
     2461 ggctacacgc tcacagccgc tgcgacaggc ctcacgtcgg ccacgagcgc cacgttcaat
     2521 atcgtggtgg ggacggcgac ccagctcgcg ttcaccacgc agcccacgaa tacgaccgcg
     2581 ggggcgacga tggccacggt ccgggtgacc gcgcgtgacg cgcagggcaa cacggcgacc
     2641 ggctacaccg gaaacgtcac cgtggcgatc gggacgaatc ccggcggcgg aacgctgtcc
     2701 ggcacgaaga cggtggcggc cgtcgccggt gtggccacgt tctcgagctt gaccatcgac
     2761 aagagcggca cgggctacac gttgaccgcg gccgcgaaca acctggccgg cgccaccagc
     2821 tccacgttca acatcaccgc cgcggcgctg agccagctcg gcttcctcgt ggaacccgtg
     2881 aataccgtgg cgggggccac gatcacaccc gccgtgaagg tcgcggggca ggacgcgttc
     2941 gggaatacgg tgacgagctt cacgggcagc gtgaccatcg cgatcacgcc gggatcgggc
     3001 accgccggcg ccacgctgag cggcaccaag acggtgaacg cctcggccgg cgtcgccacg
     3061 ttctccaccc tcagcatcga caagaccggc gccgactatc acctgacggc cagctccggt
     3121 gtgctgacac cggccacgag cgcgatcttc gccatcaccg caggcacggc gacccatctc
     3181 acgttcgagc agcagcccac gtcgaccgtc gccggcgtgg cgatcgcgcc cgccgtcacc
     3241 gtgtcggccc gggatgcgtt ggggaacccg gtgaagacgt tcgcggggag cgtgacggtg
     3301 gccatcgcgg ccgggaccgg ggcggccggc gccacgctgt ccgggacgaa ggtcgtgacg
     3361 gcgtcgggtc ccgacggtct cgccacgttc agcaccctca gcatcaacaa ggcggcgacc
     3421 gggtatcgcc tggtggcgac ggcgaccggg ctgacggcgg acacgagcac ggccttcacc
     3481 atcacggccg gcgctgcaac gaaactcgcc ttcacggtcc agccggtcgc cgcgctcgcc
     3541 ggagcggtca tcacgccggc cgtggaagtg accgcgcagg acgcgctcgg caacacggtg
     3601 accggcttca ccggcagcgt cgcgattgcg atcaccagcg ggacgggcac ggtgggggcg
     3661 gtgctcggcg gcacgacgcc ggtggtcgcg gttgcggggg tcgcgacgtt ctcggatctc
     3721 agcatcaatc tggccggcac cggctacaaa ctcaccgcca cgacgagcgg gttgacgtcg
     3781 gccaccagcg gcgcgttccc catcaactaa aagcgcctcc ggccgcgtaa agcggcgctg
     3841 gcgcaacccg tggccctctt ggtaaactgc accgtctccg cggcttacct cgaaccaggg
     3901 acgggtcccg catcgccatg cccaccgcca ccgagccgac tgcgcccccc gcccgcgggc
     3961 tggaaggcat tgtcgccaac gaatcgtcca tctgctacgt gtacggcgag gaaggccggc
     4021 tcatctaccg cggctacgac atcaacgacc tggccgatca ttccaccttc gaagaaaccg
     4081 cctacctgct gctgaacggc gacctgccga ctcgcgaccg gctcaaggat ttcgcggccc
     4141 agctcaaggc ctcgcaaaag ctcgacaagg tcgtgcagcg catcatccgg gacgcgcccc
     4201 ccaacgccaa tccgatggac gtgctccgca cggccgtgag cgcgtccgtc ttcgtggacc
     4261 cggatcgcga caacaacggg cgcgaggccg agtaccgcaa ggcggtccgc ctgatcgccc
     4321 agctgcccac catggtggcg atgttccacc ggctgcggaa cggcgagaag ccgctgtcgc
     4381 cgcgccgcgg gctctcgctc gccgccaact tcctctacct cgtctcgggg aagaagccgg
     4441 agcgggaagc cgagcacgcc ttcgacgtcg ccctcatcct ccatgccgat cacgagctca
     4501 acgcgtccac gttcgccgcc cgggtcatcg cggcgacgct ggcggacgtg cacggcgcgg
     4561 tgaccggggc gatcgcggcg ctcgcgggcc cgctgcacgg cggggccaac accgaagtga
     4621 tgaagatgct gctggagatc cagagcccgg agcgcgccga ggcgtggatc cagaacgcgc
     4681 tggcgcagcg gcacaagatc atgggattcg ggcaccgggt gtatcgcacc gaagatccgc
     4741 gtgcggcgca gctgcgccgc atggcggagg cccagggggc ccgggccggg cagctgcagt
     4801 gggccgccat ccagcggacc atcgaggacg tggtgcgccg ggagaagggc ctgtattgca
     4861 acgtcgattt ctattccgcg tcgttgtact acgtcatggg catcccgctc gacctctaca
     4921 ctcccatctt cgcggtgagt cgagtgagcg gctggtgcgc gcacgtgctc gaacagcacg
     4981 cggacaaccg cctcattcgg ccgcgcgccg agtacatggg gcacatgaac cgccgctggg
     5041 ttgcggtgcg gaagcgaggg taagcatgag cggcacgaag atcgactatc gcgacgggcg
     5101 ctgggtggtg cccgagcacc ccatcatccc gttcatcgag ggcgacggca cgggacccga
     5161 catctggcgc gcgtcgcggc gggtgttcga cgccgccgtg cggcagacct cgggcggccg
     5221 ccgccaggtg gagtggcacg aggtgctggc gggcgaaaag gcgttcaagg ccacgggcaa
     5281 ctggctgccc gacgagacgc tcgaggtgat tcgcgcgcac cgcgtagcca tcaagggacc
     5341 gctcacgacg ccgatcggcg gcggcatccg ctcgctgaac gtcgccctgc gccaggtgct
     5401 cgatctgtac gcctgcgtgc gtccggtgcg ctggttcaag ggtgtgccgg cgccggtgaa
     5461 ggatccgggc aagctcgaca tcgtgatcta tcgcgagaac gtcgaggacg tctacgccgg
     5521 ggtggagttc ccggccgaga gcagggaatc caacgagctg atcgccttgc tggagcggct
     5581 ggggaagaag gtccggcccg gcagcgcgat cgggatcaag ccgatgtcca agttcggctc
     5641 gcagcggctc gtggccaagg cgatccgctt cgcactggag tcgggccgga cgtcggtgac
     5701 gctggtgcac aagggcaaca tcatgaagtt caccgagggc gcgttccgcg attggggtta
     5761 cgaaaccgcc aagacgcagt tcggggaccg cacgatcgcg gaagccgata tgggaaaagg
     5821 gacgggggac gcgggacgag tcgtcatcaa ggaccgcatc gccgactcca tgttccagca
     5881 gctgctgctg cggcccgcgg agtactccat cctcgccacg cccaatctga acggcgacta
     5941 cctctccgac gcctgcgccg cgcaggtggg cggactcggg ctggcgcccg ggggcaacgt
     6001 gggcgacggc tacgcggtgt tcgaggccac gcacggcacg gcgcccaagt acgccgggct
     6061 ggacaagatc aaccccggga gcgtgatcct ctccggtgcc atgatgttcg ccgagctcgg
     6121 ctgggtcgag gtgagccgcg ccatcatcac cgggatcgag ggcgcgatcg gcgcggggcg
     6181 ggtcacctac gacctggcgc ggcagatgcc gggcgcgacc gaagtctcca cctccacgtt
     6241 cgccgacgcg atcatcgagc acctcgcatg aacagcacgt tgacggcgca gccgttcccc
     6301 gagatcgata ccccgctgct ggcggtggcc gtggccgccg cgaccacgct tccggcggcg
     6361 ctcgcgccgc tcgatcaagc gacgggcggc gcgctgggcc gggcgctcgc caacggggac
     6421 ttcaagggaa agcgggacga gacgctgctc gcctacggca gcgggaaggc gcgacgcgtg
     6481 ctgctcgtg
//
LOCUS       DBNGZW010000304         5355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_639773, whole genome shotgun sequence.
ACCESSION   DBNGZW010000304 DBNGZW010000000
VERSION     DBNGZW010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5355
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_639773"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(12..212)
                     /locus_tag="AC16JC_07210"
     CDS             complement(12..212)
                     /locus_tag="AC16JC_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgaP family membrane protein"
                     /protein_id="HIR5164522.1"
                     /translation="MRIHYLIRRFAGVFVLASLALGYWVSPYWFLFTAFVGANLLQSS
                     FTRWCFLERLLQRAGCGQERVA"
     gene            complement(209..499)
                     /locus_tag="AC16JC_07215"
     CDS             complement(209..499)
                     /locus_tag="AC16JC_07215"
                     /inference="COORDINATES: protein motif:HMM:NF045581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG0541 family transporter-associated protein"
                     /protein_id="HIR5164523.1"
                     /translation="MKLVMILVDADRAPDVEAILELRGLPGYTEIPQVLGKGMTGRKL
                     GTRAFPGATTLYFTVIPTAQVAGLAEELRRLREARGSAEGLRAFTLDTEELL"
     gene            complement(496..3654)
                     /locus_tag="AC16JC_07220"
     CDS             complement(496..3654)
                     /locus_tag="AC16JC_07220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5164524.1"
                     /translation="MKTGPSGALAQRFLTSKITPLLIGASLLAGVGGLLTTPREEEPQ
                     IRVPMIDVAAGLPGASPREVERRIAEPLERAIWEIPGIDYVYSASYPDGALVTARFKV
                     GTDPEVALTRLYGKLLANAGSIPPGATPPLITLHGIDEVPILTLTLSGGSDAGDGYLL
                     RQQAAELAAELKRIPDVAELWVTGGAPREVTVTLDPQALGARGLSAAVVLQALGAANA
                     SAPAGMLVSADRSVPVRVGHLLRDADQVREVVVGVSGDRAVRLREVAQVTDGPGEPQQ
                     YVGFLEGGGAGTFQPATTIVIAKRGGTNAATIGERVLHRVDELRGRVLANDVRITVTR
                     NYGATASEKARELLFHILLATLGVVLLVWAALSWREAVVVLVAIPVTLALTLLVYRLY
                     GYTLNRITLFALVFAIGILVDDAIVVVENIARHLGLKEREPAEAAVVAVDEVGNPTIL
                     ATLTVIAAILPMAFVSGLMGPYMRPIPVGASVAMLFSLAVAFIVTPYLALRLVRSHAA
                     PTGQAAGAPQAAEPRLARWYRRLLESLLGDRRRRWRAYGIVGALLLVSMALVPLKLVT
                     VKMLPFDNKSEFQLIVDMPQGTTLERTAEVAQALALTVARDAAVRDVQTYVGVPAPFN
                     FNGLVRHYFLRRGPTIADVQVNLVPKDARAASHAVALRLRPAVDSVARAYGAAVKVAE
                     IPPGPPVQATLTAEIYGPDYAAQIAAAEQVRDVFLRTGGVVDVDWSVAAPHAEVRAAV
                     AQAEAARAGTNPRDVAQTIAAAMGGDGATVGLLHDDAAAEPVAIRLELPQAARGSVGD
                     LAGLPVATARGARRLGSLAALDSAPAPQPIYHKNLRPVVYVTGDVAGKLESPVYAMLA
                     MGDTLARTGGGLPIHWATSPTLTERPLLVWDGEWRITYEVFRDLGIAFAAVLALIYLL
                     VVAWFQSFKTQLVIMAPIPLTLVGILPAHALTGAFFTATSMIGMIALAGIIVRNSILL
                     ADFVELGLERGESLHDAVIASGLVRARPIVLTAAAVVIGGVVMVSDPIFQGLGIALIG
                     GAVVATALTLVAVPLLYFEAHR"
     gene            complement(3651..4676)
                     /locus_tag="AC16JC_07225"
     CDS             complement(3651..4676)
                     /locus_tag="AC16JC_07225"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5164525.1"
                     /translation="MTRRLVLPALALALVACGSGDAALPARARVAPPPTSRVRAESLA
                     TSVPTEGSVVARRRAEIATRLMARITSVTVDAGSPVHAGQVLIRLGVEDVAANRSRAT
                     AARDAAQAARDEAVRNAARMDTLLSRDAVARVQRDQARLALVQAEAQLAMAEASLQDV
                     ATTAGYATLAAPFAGVVASRSADPGDLATPGAPLVVVEDDGPRDAVFAVPADLAAGLR
                     PGSSIPVVDPDGRSATAPVRVIARAADPQTRTVEVRVTVPRDWPSGVAVTGLVPAGWH
                     QGIAVPASAVVRRGQLTGVRVLMGDAVVLRWVRLGRMVGPSAGAGPDSARVEVLSGLE
                     PGDLIVP"
     gene            complement(4673..>5355)
                     /locus_tag="AC16JC_07230"
     CDS             complement(4673..>5355)
                     /locus_tag="AC16JC_07230"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR5164526.1"
                     /translation="VAAAADADNALAALAAFLALPDSTTLLLADPLDAALPTDSLPAA
                     MRGSRGDLAALDQGVRAAELDVRRAWATNLPSIALFGNLAQHNRAGLANGGSGDWTVG
                     VGLQWRLFPGLAGAGAIVRARAERDVAQARRDAATRQARLEIAQSERLQAAAVRRMTV
                     ARDARTEALAALDQAQLRYRTGAATISELLDVQAALTDAALDLLGARHDVLVAGALLD
                     FATGVNDQ"
BASE COUNT          792 a         2002 c         1885 g          676 t
ORIGIN      
        1 tcacgagcgg ctcaggccac ccgctcctgc ccgcacccgg ctcgctgcag cagccgctcc
       61 aggaagcacc atcgggtgaa gctcgactgg agcagattcg ccccgacgaa cgcagtgaac
      121 aggaaccagt aagggctcac ccagtagccc aaggcgagcg aagccaggac gaacacgccg
      181 gcgaaccgcc gaatcaggta gtggatcctc acaggagctc ctcggtgtcg agtgtgaacg
      241 cacgcagccc ttcggccgaa ccgcgcgctt cgcgcagccg ccgcagctcc tcggccagcc
      301 cggcgacctg cgcggtggga atgacggtga aatagagcgt cgtggcccct gggaaggcgc
      361 gcgtgccgag cttccgcccg gtcatcccct tgcccaagac ctgggggatt tcggtgtagc
      421 cgggcaaccc gcgcagctcg aggatggctt cgacgtccgg cgcgcggtcg gcgtccacga
      481 ggatcatgac cagcttcatc ggtgcgcctc gaagtagagc agcggcacgg cgacgagcgt
      541 gagtgcggtc gccacgacgg cgccgccgat caacgcgatc cccagcccct ggaagatcgg
      601 atccgacacc atgaccacgc cgccgatcac caccgcggcc gcggtcagca cgatcggacg
      661 cgcccgtacg aggcccgagg cgatcaccgc gtcgtgcagc gactccccgc gttccagtcc
      721 cagctccacg aaatcggcca gcaggatgga gttccgcacg atgatgcccg ccagggcgat
      781 catgccgatc atcgaggtgg cggtgaagaa cgccccggtc agggcgtggg ccggcaggat
      841 gccgacgagc gtcagtggga tcggcgccat gatcacgagc tgcgtcttga acgattggaa
      901 ccacgcgacc acgagcagat agatgagtgc cagcactgcc gcgaaggcga tccccaggtc
      961 ccggaacact tcgtacgtga tgcgccattc cccgtcccac acgagcagcg gccgctccgt
     1021 gagcgtcggg gaagtggccc agtggatggg gagccccccg cccgtccgcg ccagcgtgtc
     1081 gcccatggcc agcatggcgt agaccgggct ctcgagcttc cccgccacgt cgccggtcac
     1141 gtagacgacc gggcgcaggt tcttgtggta gatcggctgc ggcgccggcg cgctgtccag
     1201 cgccgccagc gacccgagcc ggcgggcccc acgggcggtg gcgacgggaa gcccggccag
     1261 atcgccgacg gagccccgcg cggcctgcgg cagctcgagc cggatggcga ccggctccgc
     1321 tgccgcgtcg tcgtgcagca gcccgaccgt cgcgccgtcg ccgcccatcg cggcggcgat
     1381 cgtctgcgcc acgtctctcg ggttggtccc cgcccgcgcc gcctcggcct gcgccaccgc
     1441 ggcgcgcact tccgcatgcg gtgcggcgac ggaccaatcc acgtcgacga cgccgcccgt
     1501 ccgcagaaag acgtcgcgga cctgttccgc cgccgcgatc tgcgccgcgt agtcggggcc
     1561 gtagatctcg gccgtgagcg tcgcctgcac cggagggccg ggtgggatct ccgccacctt
     1621 caccgccgct ccgtaggccc gcgccacgga atcgaccgcc gggcgcaggc gcagcgcgac
     1681 ggcgtgactc gccgcgcgtg cgtccttggg cacgagattg acctgcacgt cggcgatggt
     1741 ggggccgcgc cgcaggaagt agtgccgtac caggccgttg aagttgaacg gcgccggcac
     1801 gccgacgtag gtctgcacgt cgcgcaccgc cgcgtcccgg gccaccgtga gcgccagcgc
     1861 ctgcgccacc tccgcggtac gctcgagtgt cgtgccctgg ggcatgtcca cgatcagctg
     1921 gaattcactc ttgttgtcga acggcagcat cttcacggtg acgagcttca ggggcaccag
     1981 cgccatcgac acgagcagca gcgccccgac gatcccatac gcacgccagc ggcggcgccg
     2041 gtcccccagg agcgattcga gcaatcgccg gtaccagcgc gccagacggg gctcggcggc
     2101 ctgcggtgcg cccgcggctt gacccgtggg cgccgcatga ctccggacga gtcgcagcgc
     2161 gaggtacggc gtcacgatga atgccacggc cagcgagaac agcatcgcca ccgaggcgcc
     2221 gaccgggatg ggccgcatgt agggtcccat caaccccgac acgaacgcca tcggcaggat
     2281 cgccgcgatc accgtgaggg tggcgagaat cgtcgggttg cccacttcgt ccaccgccac
     2341 caccgcggct tctgcgggct cgcgctcctt cagaccgagg tgacgggcga tattctcgac
     2401 cacgacgatc gcgtcgtcca ccaggatccc gatcgcgaag accagggcga acagggtgat
     2461 gcggttcagc gtgtagccgt acagccggta gacgagcagc gtcagggcga gcgtcaccgg
     2521 gatggcgacg agcaccacga ctgcctcccg ccagctgagc gcggcccaca cgagcagcac
     2581 gacgcccagc gtggcgagca ggatgtggaa cagcagctca cgtgccttct cactcgccgt
     2641 ggcgccgtag ttgcgcgtca cggtgatgcg cacgtcgttc gcgagcaccc ggccgcgcag
     2701 ctcgtcgacg cggtgcagca cgcgttcccc gatggtcgcc gcgttggtgc cgccgcgctt
     2761 ggcgatgacg atcgtggtgg cgggctggaa ggtccctgcc ccgccgccct ccaggaaccc
     2821 gacgtactgc tgcggctcgc ccggcccgtc ggtcacctgc gccacctcgc gcaaccggac
     2881 cgcccggtcg ccgctcaccc ccaccaccac ctcccgtacc tggtccgcgt cgcgcagcag
     2941 gtgccccacg cgcaccggca cgctgcgatc cgcgctgacc agcatcccgg ccggagcgga
     3001 cgcgttcgcg gcgcccaacg cctgcagcac gaccgcggcc gagagcccgc gtgcccccag
     3061 cgcctgggga tcgagcgtca ccgtcacttc gcgcggtgcg ccgccggtca cccacagctc
     3121 cgccacgtcg gggatccgct tcagctcggc cgcgagctcc gccgcctgct ggcgcagtag
     3181 atagccgtcg cccgcgtcgc tcccacccga cagcgtgagg gtaaggatcg gcacttcgtc
     3241 gatgccgtgg agcgtgatga gcggcggcgt ggcgcccggc gggatcgacc ccgcgttggc
     3301 gagcagcttc ccgtacagcc tggtcagggc gacctccgga tccgtcccga ctttgaagcg
     3361 cgcggtgact agcgccccgt ccgggtagga cgcgctgtac acgtagtcga tcccggggat
     3421 ctcccagatc gcccgctcca gcggctccgc gatgcggcgc tccacctcgc ggggcgatgc
     3481 gccgggcagt cccgcggcga catcgatcat cggcacgcgg atttgcggct cctcctcccg
     3541 cggcgtcgtc agcaggccgc cgacgcccgc cagcagcgag gcgccgatca ggagcggcgt
     3601 gatcttggag gtgaggaagc gctgtgccag cgcgcccgac ggaccggtct tcatgggacg
     3661 atcaggtctc caggctcgag accgctcagc acctcgaccc gcgccgagtc cggccccgcg
     3721 ccggccgacg gcccgaccat cctgccgaga cgcacccagc gcagcacgac ggcgtcgccc
     3781 atcagcacgc ggacgccggt cagctgcccg cgccgcacga ccgcgctcgc cggcaccgcg
     3841 atgccctggt gccatccggc ggggacgagc cccgtgaccg cgacgccgct gggccagtca
     3901 cgcggcacgg tcacgcgcac ctcgaccgtt cgagtctggg gatcggcggc ccgggcgatc
     3961 acgcggaccg gggcggtggc gctccgtcca tcggggtcga ccaccgggat cgaggacccc
     4021 ggccgcagac cggcggcgag atcggccggg acggcgaaca ccgcgtcgcg cggaccgtcg
     4081 tcctccacca cgaccagcgg agccccgggt gtcgcgagat cgcccgggtc ggcggagcgg
     4141 ctggcgacca cgccggcgaa cggcgccgcg agcgtggcgt agccggccgt ggtcgccacg
     4201 tcctgcaggg acgcttcggc catcgcgagc tgcgcctccg cctgcaccag ggcaaggcgc
     4261 gcctggtccc gctgcacccg cgccaccgcg tcgcgactca gcaacgtgtc catgcgggcc
     4321 gcgttccgga cggcttcgtc gcgggccgct tgcgccgcgt cgcgcgcggc cgtcgcccgg
     4381 ctccggttcg ccgccacgtc ctccacgccg aggcggatca gcacctggcc ggcatgcacc
     4441 ggcgaccctg catccacggt gacgctcgtg atccgcgcca tgaggcgggt ggcgatctcg
     4501 gcgcggcgac gggcgaccac cgatccctcg gtcggcacgc tcgtggcgag cgattcggcg
     4561 cgcaccctgc tggtcggcgg cggtgccact ctggcgcgcg cgggcagggc ggcgtccccg
     4621 ctgccgcacg ccacgagggc gagggcgagc gcgggcagga cgaggcggcg ggtcattgat
     4681 cgttcactcc ggtggcgaag tcgaggagag cgcccgcgac gaggacgtcg tggcgcgccc
     4741 cgagcagatc gagcgcggcg tcggtgagag ccgcctgaac gtcgagcagc tcggaaatcg
     4801 tggccgcccc ggtccggtag cgcagctgcg cctggtcgag cgccgcgagt gcctcggtac
     4861 gggcgtcccg ggcgaccgtc atgcggcgaa cggcggctgc ctgcagccgc tcgctttgcg
     4921 cgatctccag ccgcgcctgg cgcgtcgcgg catcgcggcg cgcctgggcg acgtcccgct
     4981 cggcacgcgc ccgcacgatc gcgccggcgc ccgcgagccc cgggaacaga cgccactgga
     5041 gtcccacgcc gacggtccag tccccgctcc ccccgttggc caagcctgcg cggttatgct
     5101 gcgcgaggtt cccgaacaga gcgatcgacg ggagattcgt ggcccaggcg cggcgcacgt
     5161 ccagctcggc ggcgcggacg ccctggtcga gggcggcgag gtcgccacga gatccgcgca
     5221 tggccgccgg cagggagtcg gtggggagag cggcgtcgag cggatcggcg agcaggaggg
     5281 tggtcgaatc gggtaacgcc aggaatgccg ccaacgccgc gagggcattg tccgcgtcgg
     5341 ccgcggccgc cacac
//
LOCUS       DBNGZW010000305         2261 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_429103, whole genome shotgun sequence.
ACCESSION   DBNGZW010000305 DBNGZW010000000
VERSION     DBNGZW010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_429103"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..934)
                     /locus_tag="AC16JC_07235"
     CDS             complement(<1..934)
                     /locus_tag="AC16JC_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164527.1"
                     /translation="MTGIEQARRSNPEAATAAPGAPTLQVLHELFERLHSEGIRYCHW
                     KSNEHLQASFTGATDVDVLFDRRAIVPLTRILGETNFKRFVVKPGRGYPGIEDYVGFD
                     TASGALTHLHVHYQLTLGEKFLKGHRLPWEDVYLDTRIWNGEFGLYTADPHVELVALL
                     VRAVMKLRTRDSLLEALGTPYFRGGLRRELRWLQQRVERRQLQDIGAQLVGEPAAQLL
                     PELAQHAQPSVRQLRAFGQRVRPSLDEYRLFATNDAVRQMATRELGVVWWKVRNWYHG
                     APTKSTRTLPHGGLTVAILGADGAGKSTLTAALAD"
     gene            complement(931..>2261)
                     /locus_tag="AC16JC_07240"
     CDS             complement(931..>2261)
                     /locus_tag="AC16JC_07240"
                     /inference="COORDINATES: protein motif:HMM:NF024832.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR5164528.1"
                     /translation="ELERRHIDTAYTFSFLFGLLLGAILWFGAPVAASFPRMPEVTPV
                     MQALSFVFPLNGLQTVGQALLSRNLHFSWLANLDVVSYGIGFGVVGIVAALLGWGVWA
                     LVAAQIGQSLGRTAVLLWKHPPRLRLIFDKPAFMDLAYFGGGFTMARVANYIAVNGDN
                     MIVGRFLGAAPLGYYGRAYSLMSAPAYAFGTVLDQVLFPAMAKVQDDLKRLAAAYRRG
                     VALIALMVLPASAAIIFLAPEVIRIALGPKWGPAVAPFQVLGLGMLFRTSYKMSDSIS
                     RSTGAVYRRAWRQLIYGGLVVLGAWIGTRWGIVGVSWGALAALTVNFFLMAALGQQVA
                     GLRWRDFWGAHRPAVLLTLVSFPIVWLTTTGLRHVNAPAVVVLAVAGIVLVAVCGVAT
                     WRLPRIFLGEDGQWMASTLRSFLQKMRGRSGGDRQKVSGAFETAPVEEAR"
BASE COUNT          407 a          766 c          759 g          329 t
ORIGIN      
        1 agtcggcgag cgccgcagtg agcgtggatt ttcccgcgcc gtcggccccg aggatcgcga
       61 cggtcagccc tccgtgcggc agcgtgcgcg tgctcttcgt cggcgcccca tggtaccagt
      121 tccgcacttt ccaccagacg acgcccagct cccgggtggc catctggcgc accgcgtcgt
      181 tcgtggcgaa gagacgatac tcgtcgagcg atggccgcac gcgctgcccg aatgcccgga
      241 gctggcgcac gctcggctgc gcgtgctgcg cgagctccgg cagtagctgc gccgccggtt
      301 cgcccaccag ctgagcgccg atgtcctgca gctggcgccg ctccacccgt tgctgcagcc
      361 agcgcagctc gcggcgcagt ccgccgcgga agtagggcgt cccgagggcc tcgagcaggc
      421 tgtcacgggt gcgcagcttc atgaccgccc gtaccagcag cgccaccagc tcgacgtgtg
      481 ggtcggcggt gtacaggccg aactccccgt tccagatacg ggtgtcgagg tacacgtcct
      541 cccaggggag ccgatggccc ttcaggaact tctctccgag ggtgagctgg tagtggacat
      601 ggaggtgggt gagggcgccg ctggccgtgt cgaacccgac gtaatcctcg atcccggggt
      661 acccgcggcc gggcttgacc acgaagcgct tgaagttcgt ctcgcccaga atgcgcgtga
      721 gcggcacgat ggcgcggcga tcgaacagca cgtccacatc cgtggccccg gtgaacgacg
      781 cctgcagatg ctcgttgctc ttccagtggc agtagcgaat gccctcgctg tggaggcgct
      841 cgaacagctc gtgcaggacc tggagggtcg gagcgcccgg agcggccgtg gcggcctccg
      901 ggttcgaccg tcgggcctgc tcgatcccgg tcaacgggct tcctccaccg gcgcggtctc
      961 gaaagccccg gagaccttct gacggtctcc gccggaccgt ccacgcatct tctggaggaa
     1021 cgagcgcaac gtgctcgcca tccattggcc gtcttcgccg aggaagatgc gcggcagacg
     1081 ccaggtcgcg acgccacaca ccgccacgag gacgatgcct gcgactgcca gtacgacgac
     1141 ggccggggca ttcacatgcc gcagtccggt cgttgtgagc catacgatcg gaaaggagac
     1201 gagcgtcagc aggacggccg gacggtgtgc gccccagaag tcacgccacc ggagtccggc
     1261 tacctgctgt ccgagggcgg ccatcaggaa gaaattcacc gtcaacgccg caagcgctcc
     1321 ccacgagacg ccgacgatgc cccagcgcgt tccgatccac gcaccgagca cgaccagccc
     1381 accgtagatg agctggcgcc aggcccggcg gtagacggct cccgtcgagc gggaaatcga
     1441 atcgctcatc ttgtagctgg tacgaaacag catccccaac ccgagcacct ggaacggggc
     1501 gacggcgggt ccccatttgg gtccaagcgc gatccggatg acttcaggcg ccagaaagat
     1561 gatggctgcg ctggccggaa gaaccatgag ggcgatcagc gcgacgcccc gccggtaggc
     1621 cgcggcgagg cgcttgaggt cgtcctgtac cttcgccatg gccggaaaca gcacctggtc
     1681 gagtaccgtg ccgaacgcgt acgccggcgc cgacatcagg gaatacgcgc ggccgtagta
     1741 gcccagcggt gcggcaccca gaaaccgtcc cacaatcatg ttgtcgccgt tgaccgcgat
     1801 gtagttggcc acgcgtgcca tcgtgaaacc gccgccgaag taggcgaggt ccatgaacgc
     1861 gggcttgtcg aagatcaggc ggagacgggg tgggtgcttc cagaggagca cggccgtccg
     1921 ccccagcgac tggccgattt gtgcggctac cagcgcccac accccccatc ccagaagcgc
     1981 ggccacgatt cccacgaccc cgaacccgat cccgtagctc acgacatcga gattcgcgag
     2041 ccacgagaaa tggaggttgc ggctcagcag cgcctggccg acggtctgca ggccgttgag
     2101 aggaaagacg aaggacaggg cctgcatcac cggcgtgact tcgggcatgc gcgggaagct
     2161 ggctgcgacc ggcgcgccga accagaggat cgcccccagc aatagaccga agagaaacga
     2221 gaacgtgtag gcggtatcga tgtggcggcg ctccagctcg g
//
LOCUS       DBNGZW010000306         2415 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_702370, whole genome shotgun sequence.
ACCESSION   DBNGZW010000306 DBNGZW010000000
VERSION     DBNGZW010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_702370"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1101
                     /locus_tag="AC16JC_07245"
     CDS             <1..1101
                     /locus_tag="AC16JC_07245"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5164529.1"
                     /translation="AIMGTAAIVTPPTGALLDRYSAKPIVVAGSLASALGYAGFAFVD
                     VPWQAFACSIVSGAGIGLAGTATRQLIFTLVNREQRGASVALARVAGNFGLGLGATVA
                     GFIVASAQQLSSFQALYVFDGITYAGFALIVLTAVSAPQAATVTATDARGQGFRAVAR
                     DRLFLTVIAANLVLLVAGHTFFSNILGPFAVAHTAAGPAALGIVFLANTAFVVIAQIP
                     AVRVVARMRRTQAFAAASALFAVALLAVLPATLIPSQLAASTLLVGVAIALAIGECFH
                     LVILGPLVADMAPAHLLGRYLAVLGLMFTASQALGPAIGGLLLQASPDAVWWGGATAV
                     VLAGAVLVRLGGRLPDPLSEASSRPAVAPAVQ"
     gene            1098..2066
                     /locus_tag="AC16JC_07250"
     CDS             1098..2066
                     /locus_tag="AC16JC_07250"
                     /inference="COORDINATES: protein motif:HMM:NF006094.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine dehydratase"
                     /protein_id="HIR5164530.1"
                     /translation="MSPVTKADVAAARARLGHRVRRTPVIRVEANQLTGSHPAFWLKL
                     ESLQCTGAFKARGALNTLLAAPAIPPRGVCAASGGNHGQAVAWAARLLGVPASVFVPV
                     TCPPINLQRLADSGARVTVIGEVFEESLASAEAFAAETGALFVHPFDQAPTVAGVGTV
                     AAEFSEQVPDLDTILIAVGGGGLLAGALAALEGTAARAVAVEPSSSRCLGAALEAGEP
                     VDVAVSGPAVDSLGVRRVGQIAFDAAIARQVQHIDVTDEAITAAQLSAWQELRIGLEA
                     GGATALAALLSGAYQPQPDEVVGAVGCGGNVDIAALLSVANGGAAR"
     gene            2083..>2415
                     /locus_tag="AC16JC_07255"
     CDS             2083..>2415
                     /locus_tag="AC16JC_07255"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5164531.1"
                     /translation="MSLIRNRDFQKLWTAESISVFGSQVSQLAIPLAAAIILKVEPFA
                     FAMLGTIEFLPFILFTLPAGAWVDRMPRRPILIIGDLGRAAVLSTIPLAYFFGSLTIW
                     QLYVVAFVT"
BASE COUNT          301 a          857 c          819 g          438 t
ORIGIN      
        1 gcgatcatgg ggacggccgc aatcgtcacg ccgccaacgg gggcgttgct cgaccgctac
       61 tccgcgaaac cgatcgttgt cgcaggaagc ctggcgagcg ccctggggta cgcgggcttc
      121 gccttcgtgg acgttccctg gcaggcgttc gcgtgctcga tcgtcagcgg agctggaatc
      181 ggcctggcag ggacggccac tcggcaactg atcttcacgc tggtcaaccg cgagcagcgc
      241 ggtgcatccg tcgcccttgc ccgcgtcgcc ggcaacttcg gtctcggtct cggcgcgacg
      301 gtcgccggct tcatcgtcgc atcggcccag cagctcagct cgttccaggc gctctacgtc
      361 ttcgacggca tcacgtacgc gggttttgcg ctgatcgtgc tcaccgccgt ctccgccccg
      421 caagccgcga ccgtcaccgc caccgacgcc cggggccagg gctttcgcgc cgtcgctcga
      481 gatcggctct tcctcacggt gatcgccgcc aatctggtgc tgctggtcgc cggccacacg
      541 ttcttctcca acatcctggg tccgtttgcg gtggcccaca cggccgctgg cccggcggcg
      601 ctcgggatcg tcttcctcgc caacacggcc tttgtcgtga tcgcgcagat cccggcggtg
      661 cgggtcgtgg cgcgaatgcg acgcacccag gcgttcgcgg cggcgagcgc gctgtttgcg
      721 gtcgccttgc tggcggtgct gccagcgacg ctcatcccct cgcagctcgc ggcgagcact
      781 ctcctcgtcg gcgttgcgat tgcccttgcc atcggtgagt gcttccacct tgtcattctc
      841 gggcccctcg tcgccgacat ggccccggcc catctgctcg gccgctatct ggcggtcctc
      901 ggtctgatgt tcaccgcgtc gcaggcactc ggtcccgcga tcgggggcct tctcctgcag
      961 gcctcgccag acgccgtctg gtggggtggc gcgacggcag tggtcctcgc cggagccgtc
     1021 ctcgtgcgac tcggcggtcg ccttcccgac ccgctcagcg aagcatcgtc acgtcctgcg
     1081 gtcgcaccgg cggttcagtg agtcccgtca cgaaggccga tgtcgctgcg gcacgggctc
     1141 gactgggtca tcgggtccgg cggacgcccg tcatccgcgt cgaggccaat cagctgaccg
     1201 gctcgcatcc ggccttctgg ctcaagctcg agtcattgca gtgcaccggg gcgttcaagg
     1261 cgcggggcgc attgaatacg ctactggccg ccccagcgat accccccaga ggtgtctgcg
     1321 cggccagcgg gggtaatcac gggcaggcgg tcgcgtgggc agctcgactg ctgggtgtcc
     1381 cggcgtccgt gttcgtgccg gtgacgtgcc ccccgatcaa cttgcagcgg ctggccgact
     1441 ccggcgcgag ggtcaccgtc atcggcgagg tcttcgagga aagcctcgcc agcgccgaag
     1501 cgttcgcggc cgagaccggc gcgctgttcg tgcacccttt cgatcaggcg ccaacggtcg
     1561 cgggtgtcgg gactgtagcc gcggaattca gcgaacaggt ccccgacctg gacacgatcc
     1621 tgatcgcggt cggcggcggc ggtctcctgg ctggtgcgct tgccgcactc gagggtaccg
     1681 ccgctcgtgc ggtggccgtc gagccgtcgt cgtcacggtg ccttggcgcc gcgctggagg
     1741 ctggcgagcc ggtcgacgtg gccgtgtcgg gccccgccgt cgacagtctc ggcgtgcgcc
     1801 gggtcgggca gatcgcgttc gacgctgcca tcgcacgcca ggtacagcac atcgacgtca
     1861 ccgacgaggc catcacggcc gcgcagctca gcgcctggca ggagctgcga atcggccttg
     1921 aggcgggtgg agcgacagcg ctggccgcac tgctcagcgg cgcctaccag ccgcagcccg
     1981 acgaagtggt cggcgcagtg ggctgtggcg ggaacgtcga catcgcggcg ctgctgtcgg
     2041 tggcaaacgg cggagccgca cggtgatgtg gggtagctgc tcatgagcct gatccgtaac
     2101 cgcgacttcc agaagctctg gacggccgag tcgatcagcg tcttcgggtc gcaggtcagc
     2161 cagctcgcca tccccctggc ggcggccatc atcctgaagg tcgagccgtt cgcgttcgcg
     2221 atgctcggca cgatcgagtt cctgccgttc atcctgttca cgctgccggc gggcgcctgg
     2281 gtcgatcgca tgcctcgcag gccgatcctg atcatcggcg acctcggcag ggcggccgtc
     2341 ctgtcgacga tcccgctcgc ctacttcttc ggcagcctca ccatctggca gctgtacgtc
     2401 gttgccttcg tcacc
//
LOCUS       DBNGZW010000307         8798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1597580, whole genome shotgun sequence.
ACCESSION   DBNGZW010000307 DBNGZW010000000
VERSION     DBNGZW010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1597580"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..823)
                     /locus_tag="AC16JC_07260"
     CDS             complement(167..823)
                     /locus_tag="AC16JC_07260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005664884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5164532.1"
                     /translation="MTHLATRRPVRRRAKPRWQRRKDARPEEIVAAALEEFVERGFAA
                     TRVEDVARRAGVTKGTVYLYFKNKEALFKAVVRETIVPVIAKGESIARAFQGSARELL
                     EQLVREYWRLVYETALAGIPKLMMAEAANFPELARFYYEEVVTRGHLLLAGVIERGIR
                     NGEFRRVDVRVAAKLALSPLMHATVAQRAFAHCMPEGFNVRKYLDTHIDLYLHGITHA
                     "
     gene            complement(932..1918)
                     /locus_tag="AC16JC_07265"
     CDS             complement(932..1918)
                     /locus_tag="AC16JC_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR5164533.1"
                     /translation="MKVIVPLAGKGTRLLPLTKRVPKPLVHVAGRPVMDYVMDTLAGF
                     DVEELLVITGHLKEDVERYIRRHYRIPARFVEQRTLDGTAGAINLARPWVDGPVLIIF
                     VDTLFDADLSLIRTADADGILWAKEVEDYQRFGVIVTDADGYMRRIVEKPDTPVSRLA
                     NIGLYYIRDWRTLFDGIAHVLGQAAGKGGEFYLTDAFQYMVDHGKRLLTARVGGWYDC
                     GKVDTLLETNEHLLRTGRGLVPPAAPGVVIREPVRIEPGVTLRDCTVGPNVTVEGGSV
                     IEGSTVAHAILGRQVVLRRARVERSVIGDEQVLEDRAIADSVMDGGEIAAAR"
     gene            complement(1915..2268)
                     /locus_tag="AC16JC_07270"
     CDS             complement(1915..2268)
                     /locus_tag="AC16JC_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5164534.1"
                     /translation="MSELPYRVEKPWGHEVIWARTDRYVGKILHVEAGQLLSLQYHER
                     KDESIYVLAGEIILRLQQGDTLIERRLGPGEAFHIHPKLVHQFEAVVTSDLLEASTPE
                     IDDVIRLKDRYGRVP"
     gene            complement(2265..2846)
                     /locus_tag="AC16JC_07275"
     CDS             complement(2265..2846)
                     /locus_tag="AC16JC_07275"
                     /inference="COORDINATES: protein motif:HMM:NF024247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5164535.1"
                     /translation="MAEDAGNRRTFFRDAFGRLAREVARRAADRVVARRWFRPPGAAP
                     EVAFLASCTRCGACVDVCPAHAIRRAPASAGLAAGTPILEPSIQACVVCVDIPCARAC
                     PTSALVVPPDGWDGIRLGVLSLDVDRCITFHGASCGVCARSCPVGERALAMDRGGRPV
                     LKPEGCVGCGVCVTACVTTPSSLSLALVPEELL"
     gene            complement(2848..5826)
                     /locus_tag="AC16JC_07280"
     CDS             complement(2848..5826)
                     /locus_tag="AC16JC_07280"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR5164536.1"
                     /translation="MLFFLALFALKDSLAVASGGASPRDSLAAPYWQQQVAYRITAAL
                     DEPSGVLTGHARIVYVNRSPDTLSDFYVHQYLNAFRPASRWSAVDSAEGRVRFQHLAD
                     PDYAFERITAASIMGADSRPDYPFAPDSTVAHWRLPRPLAPGDSLVAEIDWQARPSTL
                     PRRQGRRGRRFDFAQWYPKVVVYDAHGWEAHPLYPAGEFYGEFGTFDVTLDLAADQVI
                     GATGVPVCGDPGWERARALTAEPVDYQRDYYGAVPNDCPAAAPGRKAVRFRAEQVHHF
                     AFSLNPQYVYEQGRYGGVVVRVLYQPGDTASWGHGIAVRRTETALAWLDSLYGRFAWP
                     QMTNVHRIEGGGTEFPMMVMNGGPSLGLILHEVGHNYTMGVLANNEWREGWLDEGFTS
                     FQTAWYGETHGQGDEYPDLEAGILFDDLDRWSEPVSMVSERYRDFFVYNEMIYNKAQL
                     FYEQLRYVVGDDVMRRILRTWYERWQLKHVDEDKFRAVCQEVSGQDLGWLFGQWLHGT
                     PLIDYRLERVERRRLPDGRWSVTATIRRLGDGWMPVEIGDARRIYARATGQPEVERVE
                     FTADAKPGRLMLDPRARAHDWNMLNNAEPRGLFRDRARRQWRLDDAAHEAVRRDGLMV
                     GFLPVGWYNDAGGVTLGIRSRANYLGRYERHLFLGTTGTRSDAPQRSAFYGRITDPVA
                     QLMPRTESKIAGWSAEGRAGVAFSMDRSLRERWVDRADPHAGFDALWMATTNLAFLDP
                     RLWDDAGTVEAGPWFATSWPAGRTALHTRVGVRGGVVYRYPGPGVTSKDRYDMEGFLR
                     GTAELSARRPFVGGTRLGVRAFAGGYLAHSPPPLQRRIYLAGADPYETFTNPLLRSRG
                     ALFVRPDFHYQAPGDANLRGFAPELGGRWALSANLELSRSLLSRRRGALRSVALEAFG
                     DAGVVDSAAVPPASGRGAATTLWDAGIGLVSTHQVGDLGWTMRFELPLFVNRWEFAAD
                     SDAGDGRTAFRWSFSLAPSF"
     gene            5989..7197
                     /locus_tag="AC16JC_07285"
     CDS             5989..7197
                     /locus_tag="AC16JC_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164537.1"
                     /translation="MSRTHLLLACGLVLGAAACVDRPAPVATDDGTPGVAGPVAAPGA
                     VGPRPERLARLFAGALRSPAFRTYLKAQLDASPFPEHKIQLQRFLAAPDGRALRDLAE
                     QNGTTEADVQQEARAAIPLEVYLPVPAHRAAWSGNDNVLVATAILDREPPVAFSPDGR
                     RSILSPDTPPATPVIAVVPVETEFDASPDRTTCLDCSGGGAGGGTTSFIGLFMTKAHF
                     DGDYEGWLKGDPEYEIHVLGQSGSTDSLTDYQCAGEHAGGPYAFDQNSNDWTGSVMLF
                     SQQQLDSYKAAHPGQALRVLALEDDDTACKIIMNKDDLNRVLRFVDSVYSKSTGGRDT
                     TSSIGKIYKWAKVLRNIWTLIGSIIKTNDDLIGTAIEDDVVGTIYPGYGWIVKGSNNV
                     THGMLNLEMR"
     gene            7217..7819
                     /locus_tag="AC16JC_07290"
     CDS             7217..7819
                     /locus_tag="AC16JC_07290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164538.1"
                     /translation="MRFATTVVLVCLALLTGPAKAQFGLSSDVQLSIYGGVSRDTSAG
                     NLGQTFRPYRPILVTLRPEWTIGRMRVALGVSYGTPDIAEDGDPLALILHNTATLLEL
                     SPELSYRLVQLPRGSTIRLHAGPLISIWNLKADEGARTRSGALLAASTEFPLTSRLHG
                     VIRLSGAVSSRLFRQSDLPPEFELQGVRRASIGLGLRYGP"
     gene            complement(7888..8430)
                     /gene="atpH"
                     /locus_tag="AC16JC_07295"
     CDS             complement(7888..8430)
                     /gene="atpH"
                     /locus_tag="AC16JC_07295"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR5164539.1"
                     /translation="MKSATVARNYAEALLAAGEQLGQTELFGRLMDALAGAIQSDETI
                     AVVLDSPRVAKPVKSRLLAEALEGTAPVEFVRFLQAVVRRGRQGLLGEIAQQYQALVD
                     VKENRVHAGVTLATPAGDALQRAIVERLGRLLGKEVRAHFRPDPRILGGMVGRVGERV
                     YDGSLKRRLNLLRRKMLTGE"
     gene            complement(8427..>8798)
                     /gene="atpF"
                     /locus_tag="AC16JC_07300"
     CDS             complement(8427..>8798)
                     /gene="atpF"
                     /locus_tag="AC16JC_07300"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR5164540.1"
                     /translation="EARIAKQLGEAEKANAEAARLLEEHRAALAKARSEAQEILAGAK
                     SVAQKEREQLLAKTREEQDQLLERARREIEAEKEKAILALRREAVDLSIAAASKLVES
                     NLDSDTNRRLVLDYLTSLERH"
BASE COUNT         1383 a         3179 c         2920 g         1316 t
ORIGIN      
        1 cccccgcgcc tccgcggccc gcaggccggc cagggtcacg gccggtgcgc cgaccccggc
       61 gcggcccacc gcgtcggcga aggacagccg cagccccacg gcgggggtct gcgccgcact
      121 actcccggcg gccgcgatga gcgccgcgat ggcgatgagg aaggatctac gcatgcgtga
      181 taccatggag atagagatcg atgtgcgtgt cgagatactt gcggacgttg aaaccttccg
      241 gcatgcagtg ggcgaaggcg cgctgcgcta cggtggcgtg catcagcgga gacagggcga
      301 gcttggcggc gacgcgcacg tccacgcgcc ggaactcgcc gttcctgatg ccgcgctcga
      361 tcacgccggc cagcagcagg tgcccacgcg tcaccacctc ctcgtaatag aaccgggcca
      421 gctcggggaa gttcgcggcc tcggccatca tcagcttggg aatccccgcg agcgccgtct
      481 catacaccag gcgccagtac tcgcgcacca gctgctcgag cagctcgcgc gcgcttccct
      541 gaaacgcccg ggcgatggac tcgcccttgg cgatcacggg cacgatggtc tcgcgcacga
      601 ccgccttgaa cagggcttcc ttgttcttga agtagaggta caccgtgccc ttggtgacgc
      661 cggcgcggcg cgccacgtcc tcgacccggg tggcggcgaa gccgcgctcg acgaactctt
      721 cgagcgcggc ggccacgatc tcctcggggc gggcgtcctt gcgccgctgc cagcgcggct
      781 tggcgcggcg gcggacggga cggcgggtag cgaggtgtgt catggtgaaa cgcctcacgg
      841 gttacttact gaagggttag taagtaaacg gtcggcgccg agttcacaag ggaggctcag
      901 cttcgccatg taaggaaccg gtgaggcgga gtcagcgggc ggcggcgatc tccccgccgt
      961 ccatcacgct gtcggcgatg gcccggtctt cgagcacctg ctcgtcgccg atcaccgagc
     1021 gctcgacccg cgcccggcgc agcaccacct gccggccgag gatggcgtgg gccaccgtgc
     1081 tcccttcgat cacgctgccg ccctcgaccg tgacgttggg ccccaccgtg cagtcgcgca
     1141 gcgtcacccc cggctcgatc cgcaccggct cccggatcac gacgcccgga gccgccggcg
     1201 ggacgaggcc ccgcccggtc cgcagcaggt gctcgttcgt ctcgagcagc gtgtccacct
     1261 tgccgcagtc gtaccagccg ccgaccctcg cggtgagcag ccgcttcccg tggtccacca
     1321 tatactggaa cgcatcggtg aggtagaact cgcccccctt ccccgccgcc tgtcccagca
     1381 cgtgggcgat gccgtcgaac agcgtgcgcc agtcccggat gtagtacagc ccgatgttcg
     1441 cgaggcgcga caccggcgta tcgggcttct ccacgatgcg ccgcatgtag ccgtcggcat
     1501 cggtcacgat gacgccgaag cgctggtagt cctccacctc cttggcccac aggatcccgt
     1561 cggcgtcggc cgtacgaatc agcgacagat cggcgtcgaa tagcgtatcg acgaagatga
     1621 tcagcaccgg cccgtccacc cacggccgcg ccagattgat cgcgcccgcc gtgccgtcca
     1681 gcgtccgctg ctccacgaac cgcgcaggga tccggtagtg gcgccggatg tagcgctcga
     1741 cgtcctcctt caggtgcccc gtgatcacga gcagctcttc gacgtcgaac ccggcgaggg
     1801 tgtccatcac gtagtccatc accggccggc cggccacgtg caccagcggc ttgggcacac
     1861 gcttggtgag gggcagcaac cgcgtgccct tcccggcgag cgggacgatc accttcacgg
     1921 caccctcccg tagcgatcct tgaggcggat cacgtcgtcg atctccggcg tggaagcctc
     1981 gagcaggtcg ctcgtcacga ccgcctcgaa ttggtgaacc agcttgggat gaatgtggaa
     2041 cgcttcgccc ggtcccaggc ggcgctcgat cagcgtgtcg ccctgctgca gccgcaggat
     2101 gatttcgccg gccagcacgt agatggattc atccttgcgc tcgtgatact gcaggctcaa
     2161 cagctggccc gcttcgacgt gcaggatctt ccccacgtaa cggtcggtgc gcgcccagat
     2221 cacctcgtgg ccccagggct tctccacccg gtacgggagc tcgctcacag cagctcctcc
     2281 ggcacgagcg ccagggaaag cgacgacggc gtggtcacgc aggccgtcac gcacaccccg
     2341 catcccacgc acccctccgg cttcagcacg ggacgcccgc cgcggtccat cgccagcgcc
     2401 cgctcgccga ccgggcacga ccgcgcgcac acgccgcagc tggcgccgtg aaaggtgatg
     2461 caccggtcca cgtccaggct gagcacgccc agccggatac cgtcccatcc gtcgggcggc
     2521 accaccaggg cgctcgtggg acaggcccgg gcacagggga tgtccacgca taccacgcag
     2581 gcctggatcg acggctcgag aatcggcgtc cccgcggcca gccccgcact cgcgggcgcc
     2641 cgccggatcg cgtgcgcggg gcagacatcc acgcacgccc cgcaccgcgt gcacgacgcc
     2701 aggaacgcca cctcgggggc ggcgcccggc ggccggaacc agcgccgcgc cacgacacga
     2761 tcggcggccc ggcgcgccac ctcgcgcgcc aggcgcccga aggcgtcccg gaagaaggtg
     2821 cggcggttcc cggcgtcctc ggccatctca gaagctcggc gccagtgaga acgaccagcg
     2881 aaacgcggtc cggccgtcac cggcgtcgct atccgccgcg aactcccagc ggttgacgaa
     2941 caggggcagc tcgaaccgca tcgtccaccc caggtcgccg acctgatgtg tggacacgag
     3001 gccgattccg gcgtcccaca gggtcgtcgc cgcgccccgt ccggaggccg gaggaacggc
     3061 cgcggagtcc accacgccgg cgtcaccgaa tgcttcgagg gcgacgctgc gcagcgctcc
     3121 ccgccgccgg ctcagcaggc tgcggctcaa ctcgaggttg gcgctcagcg cccagcggcc
     3181 gcccagttcg ggcgcgaagc cccgcagatt ggcgtctccc ggagcctgat aatggaaatc
     3241 gggccggacg aacagggcac cacggctgcg cagcagcggg ttggtgaacg tttcgtaggg
     3301 gtcggcgccg gcgaggtaga tgcgccgctg cagcggcggg ggcgagtgcg ccaggtagcc
     3361 ccccgcgaag gcccgcacgc cgagccgcgt gccgcccaca aacgggcggc gcgccgagag
     3421 ctcggccgtc ccccggagga acccttccat gtcgtagcgg tccttcgagg tgacccccgg
     3481 accggggtag cggtagacga cgccgccccg gacgccgacg cgagtgtgca gcgccgtgcg
     3541 gccggccggc cagctcgtgg cgaaccaggg accggcctcc acggtgccgg catcgtccca
     3601 cagccggggg tcgaggaagg ccaggttcgt cgtggccatc cagagcgcat cgaatccggc
     3661 gtgcggatca gcccggtcca cccagcgctc gcgcaatgac cggtccatgg agaacgcgac
     3721 gccggctcga ccctctgccg accatcccgc gatcttcgat tcggtgcgcg gcatcagctg
     3781 cgcgacggga tcggtaatgc ggccgtagaa cgcgctgcgt tgcggcgcgt cgctgcgtgt
     3841 cccggtcgtg cccaggaaca ggtgccgctc gtaacgcccg aggtagttcg cccggctccg
     3901 gatgccgagc gtcacccccc ccgcatcgtt gtaccagccc accggcagga agccgaccat
     3961 gagcccgtcg cgccgcaccg cttcgtgcgc cgcatcgtcg agccgccact gtcgccgcgc
     4021 ccgatcccgg aacaggcctc gcggctctgc gttgttcagc atgttccagt cgtgcgcgcg
     4081 cgcccgcggg tcgagcatca gccggcccgg cttcgcgtcg gcggtgaact ccacgcgctc
     4141 tacctcgggc tggccggtgg cccgggcgta aatgcggcgc gcgtccccga tctcgaccgg
     4201 catccagccg tcgccgaggc gccggatcgt cgccgtgacg ctccacctcc cgtcaggcag
     4261 acggcggcgc tcgacgcgct ccagtcggta gtcgatcagc ggcgtcccgt gcaaccattg
     4321 cccgaagagc cacccgaggt cctgccccga gacctcctgg cacaccgccc ggaacttgtc
     4381 ctcgtccacg tgcttcagct gccagcgctc gtaccaggtg cgcaggatgc ggcgcatgac
     4441 gtcgtcgccc accacgtagc gcagctgttc gtagaacagc tgcgccttgt tgtagatcat
     4501 ctcgttgtag acgaagaagt cgcggtagcg ctcgctcacc atcgacaccg gctcgctcca
     4561 gcggtccaga tcgtcgaaca ggatgcccgc ctcgaggtcc ggatactcgt ctccctgacc
     4621 gtgcgtctcg ccgtaccagg ccgtctggaa gctggtgaac ccctcgtcca gccatccctc
     4681 gcgccactcg ttgttggcga ggacccccat cgtgtaattg tggccgacct cgtgcaggat
     4741 gagacccagc gacgggccgc cgttcatcac catcatgggg aattcggtgc cgccgccttc
     4801 gatgcggtgc acgttggtca tctggggcca ggcgaaccgc ccgtagagcg aatcgagcca
     4861 ggcgagcgct gtttcggtgc ggcgcaccgc gatcccgtgc ccccagctcg ccgtgtcccc
     4921 tggctggtac agcacgcgga ccacgacgcc gccgtagcgg ccctgctcgt agacgtactg
     4981 cgggttgagc gagaacgcga agtgatgcac ctgctcggcg cggaagcgca ccgccttgcg
     5041 gcccggcgcc gctgcgggac agtcgttggg cacggccccg tagtagtcgc gctggtagtc
     5101 caccggctcg gccgtgagcg cgcgggcccg ttcccagcca gggtcgccgc acaccggcac
     5161 gccggtcgcc ccgatcacct ggtccgcggc gagatcgagc gtcacgtcga acgtgccgaa
     5221 ctcgccgtag aactcccccg ccggatagag gggatgcgct tcccagccgt gggcgtcgta
     5281 caccacgacc ttgggatacc actgcgcgaa atcgaagcgc cggccgcggc ggccctggcg
     5341 gcgcggcagc gtcgagggac gcgcctgcca gtcgatctcc gccaccagcg aatcgccggg
     5401 ggccaggggt cgcgggaggc gccagtgcgc cacggtcgaa tcgggcgcga acgggtagtc
     5461 gggccgagag tccgcgccca tgatgctcgc cgcggtgatg cgctcgaacg cgtagtcggg
     5521 gtccgcgaga tgctggaagc gcacccgccc ttcggccgaa tccaccgcgc tccagcgcga
     5581 ggcgggccgg aaggcgttga ggtactggtg cacgtagaag tcggacagcg tatcgggcga
     5641 gcggttcacg tacacgatgc gggcgtgtcc cgtcagcacc cccgagggct catccagcgc
     5701 ggccgtgatg cggtaggcga cctgttgttg ccagtagggc gcggcgaggg agtccctggg
     5761 ggacgcgccg ccggaggcga ccgccaggga gtccttcagg gcgaagaggg ccaggaagaa
     5821 cagcatcgag cggggaagct acacacggcc gggcgggccg gggaagcgga acgcgtgttt
     5881 tcggcgcgta ccgggtgatt cggacgacac atcgacgcga ggcctcgcgc aggttgtggc
     5941 cccggcgccc gggtgggcgc cggggaaacc ctgagccagg agtgatcgat gtctcgcaca
     6001 caccttctcc tcgcctgcgg cctggtattg ggcgccgcag cctgcgtgga ccggcccgcg
     6061 cccgtcgcga ccgacgacgg gacgccgggc gtggcggggc ccgtcgccgc gcccggagcg
     6121 gtgggccccc ggcccgagcg gctcgcccgc ctgttcgcgg gggcgctgcg cagccccgcg
     6181 tttcgcacct acctgaaggc gcagctcgac gcgtcgccct tccccgagca caagatccag
     6241 ctgcagcgtt ttctcgccgc gccggacggc cgagcgctac gggacctcgc cgagcagaac
     6301 ggcaccaccg aggccgacgt gcagcaggaa gcccgggccg ccatcccgct cgaagtgtat
     6361 ctgccggttc cggcccatcg tgctgcgtgg agcggaaacg acaacgtgct ggtggccacg
     6421 gccatcctgg atcgcgagcc tccggtggcg ttctctccgg atggccgacg atcgatcctc
     6481 agccccgata caccgcccgc aacgcccgtc atcgcggtgg tgccggtgga gaccgagttc
     6541 gacgcctcac cggaccgcac gacttgcctg gactgttccg gcggtggggc agggggcggg
     6601 accaccagtt tcatcggctt gttcatgacg aaggcgcatt ttgacggaga ttatgagggt
     6661 tggttgaagg gcgaccccga gtatgagatc cacgtgctcg ggcaatcggg gagcaccgac
     6721 tcactgacgg actaccagtg cgccggggag catgcaggag ggccgtacgc cttcgatcag
     6781 aacagcaacg actggacggg cagcgtgatg ctgttcagcc agcagcaact cgacagctac
     6841 aaggcggcgc acccggggca ggcgctgcgc gttttggcac tcgaggatga cgacacggcg
     6901 tgcaagatca tcatgaacaa ggacgacctc aatcgtgtcc tgcggttcgt ggatagtgtc
     6961 tactcgaaga gcacaggcgg tcgtgacaca acgagcagca tcggcaagat ctacaagtgg
     7021 gccaaggtgc tccgcaacat ttggaccctg atcggctcga tcatcaagac gaacgacgat
     7081 ctgatcggca ccgcgatcga agacgacgtc gtaggcacca tctatccggg ctacggttgg
     7141 atagtaaagg gcagcaacaa cgtcacgcac ggaatgctca acctggagat gcgctgaaat
     7201 gaccggagaa tggcacatgc gattcgcgac gacggtggtc ttggtctgct tggccctcct
     7261 gactgggccg gcgaaggcgc aattcggcct atcgagtgac gtgcagcttt ccatctatgg
     7321 cggagtatcc cgcgacacgt cggcagggaa tctcgggcag acgtttcgac cctatcggcc
     7381 gatccttgtg acattgcgcc cggagtggac catcggacgg atgcgcgtcg ccctgggcgt
     7441 atcgtacgga accccagaca tcgccgagga cggcgacccg ttggctctga ttctccacaa
     7501 tacggccacg ctcctcgagt tgtcaccaga gctctcgtac cggctcgtgc aattgcctcg
     7561 cggatcgacg atccgtttac acgcgggccc cctgataagc atctggaatc tgaaagcgga
     7621 cgagggggcc cgtacccgat ccggtgcact tctggcagcc tcgaccgagt tccccctcac
     7681 ctcgaggctt catggagtga tccgcctgag cggtgcagtg tcatcgaggc tgttccgcca
     7741 atccgatctt cccccggaat tcgaactcca gggcgtgcgc cgcgcgagca tcggcctggg
     7801 cctgcggtac ggtccctgaa atgcgacggc ccggggctct gtcgccccgg gccgccgctc
     7861 gtcccgcgtc ccccctcccc cgtccctcta ctcccccgtc agcatcttcc tgcggaggag
     7921 attcagccgc cgcttgagcg agccgtcgta cacccgctcg ccgacgcgtc ccaccatgcc
     7981 gcccaggatg cgtggatcgg gacggaagtg cgcgcgcacc tccttgccca acagccgccc
     8041 cagccgctcc acgatggccc gctgcagtgc gtcgccggcg ggcgtggcga gggtcacccc
     8101 ggcgtgaacg cggttctcct tcacgtccac cagcgcctgg tactgctgcg cgatttcccc
     8161 cagcagcccc tggcggccgc gccgcaccac cgcctggaga aaccgcacga actccaccgg
     8221 cgccgtccct tccagcgcct ccgcgaggag acgcgacttc acaggcttgg ccacccgcgg
     8281 cgagtcgagc accacggcga tcgtctcgtc cgactggatg gcgccggcga gcgcgtccat
     8341 caaccgcccg aagagctccg tttggccgag ctgctcgccg gccgccagca gcgcctcggc
     8401 gtagttcctg gcgaccgtcg cgctcttcaa tggcgctcga gcgacgtcag gtaatccagc
     8461 accaggcggc ggttcgtgtc gctgtcgaga ttcgactcga ccagcttcga cgccgcggcg
     8521 atcgagagat cgaccgcctc gcgccgcagc gccagaatcg ccttctcctt ctcggcctcg
     8581 atctcgcgcc gcgcgcgctc cagcagctgg tcctgctcct cgcgcgtctt cgccagcagc
     8641 tgctcccgct ccttctgcgc cacggacttg gcgccggcga gaatctcctg cgcctcggac
     8701 cgcgccttgg cgagcgccgc gcggtgctcc tccagcaggc gggcggcctc cgcgttggcc
     8761 ttctccgcct cccccagctg cttcgcgatc cgcgcctc
//
LOCUS       DBNGZW010000308         1650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1663151, whole genome shotgun sequence.
ACCESSION   DBNGZW010000308 DBNGZW010000000
VERSION     DBNGZW010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1663151"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1206)
                     /locus_tag="AC16JC_07305"
     CDS             complement(<1..1206)
                     /locus_tag="AC16JC_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018246607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR5164541.1"
                     /translation="MARLLSVNVGLPRDIEWNGRIVHTGIWKDPARDRCKVGRLNLEG
                     DGQGDLAGHGGEQRAVFVYQIESYRHWQEQLGRTDFVHGQFGENFTVEGLADDAVGIG
                     DRYRIGSALFEVTQPRVTCYRVGIRMNEPRMPALLTGSGRPGFYFRVLQEGEVGAGDE
                     IVKEGEAAERMTVAEINALLYLPDHARDRLERALRIEALSHGWRSAFEALLRSHATGN
                     AGLAPAAAVHPAAPGFRPLRVTATDQESADVLSLTLQSPSGQPLPTALPGQYVVLRLQ
                     RAAGGPPLFRAYSLSGPPSTERYRISVKIEPNGAAGPYLREHVRVGDALDVSSPRGSF
                     ILESGERPVALLSAGIGATPVLAMLHALAAARSTRQVLWLHGARDGQHHPFGAEVRRL
                     TQALKQGRSY"
     gene            1362..1466
                     /locus_tag="AC16JC_07310"
                     /pseudo
     CDS             1362..1466
                     /locus_tag="AC16JC_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140731.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
     gene            1463..>1650
                     /locus_tag="AC16JC_07315"
     CDS             1463..>1650
                     /locus_tag="AC16JC_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020712684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5164542.1"
                     /translation="MNDQREREIRAALDQHWAASDANDFDTEHRIYHEDAVLEYPQSG
                     ERTRGRRNIQSQRASQPS"
BASE COUNT          278 a          607 c          522 g          243 t
ORIGIN      
        1 ataggagcgg ccctgcttga gcgcctgcgt gaggcggcgg acctcggcgc cgaacggatg
       61 gtgctgtccg tcgcgcgccc cgtgcagcca caggacctgt cgcgtcgagc gggccgcggc
      121 gagcgcgtga agcatcgcca gaaccggcgt cgccccgatc cccgcgctga gcagcgccac
      181 cggccgctct ccggactcca ggatgaagct cccgcgcggt gagctgacgt cgagcgcatc
      241 gcctacccgg acatgctccc gcaggtaggg tccggccgcc ccgttcggct cgatcttcac
      301 gctgatgcgg tagcgctccg tcgacggggg gcccgagagg gagtaggcgc gaaacagcgg
      361 cgggccgccg gctgcccgtt gaagacgcaa gaccacgtac tggccgggca gcgccgtcgg
      421 caacggctgg ccgctcgggc tctgcagcgt cagcgacagg acgtccgcgg actcctgatc
      481 ggtcgccgtc accctgagcg gccgaaatcc cggagcggcc ggatgcaccg ccgcggccgg
      541 ggcgagcccc gcatttccag tcgcgtggct ccgcagcaac gcctcgaacg ccgagcgcca
      601 tccgtgcgac agcgcctcga tccgcagcgc gcgctccagt cgatcgcgcg catgatcggg
      661 gaggtagagg agcgcgttga tctccgcgac cgtcatccgc tctgccgcct cgccctcctt
      721 cacgatctcg tcgccggcgc ccacctcgcc ttcctgcaga actcggaagt agaaccccgg
      781 ccggccactt cccgtcagca aggccggcat ccgcggctcg ttcatccgga tgcccacgcg
      841 atagcaggtc acacgcggct gcgtcacctc gaacagcgcg ctgccgatcc gatagcgatc
      901 gccgatgccc accgcgtcgt cggccagccc ctcgacggtg aagttctcgc cgaactgccc
      961 gtgcacgaag tcggtccggc caagttgctc ctgccagtgc cggtacgact cgatctggta
     1021 gacgaagacc gcccgctgct cacccccgtg cccggccagg tcgccttgcc cgtctccctc
     1081 gagattcagc cggccgacct tgcagcggtc acgcgcggga tccttccaga tcccggtgtg
     1141 cacgatgcgg ccattccact cgatatcgcg tggaagtccc acattcaccg acaggagccg
     1201 agccacgttc cgtgacgtac gccccgaacg cgacagtgtc aagatattgc tctgaccccc
     1261 gcacaatctc ggctcgactc tgcggcctag tgcgagcatt cttctgcacg gaggaggttg
     1321 ctatggttcc gacagccggc gctcaccagc tcgggccgcc ccatttccac tgggcgagat
     1381 ccggcgagta cgtcacccag ataaccgcga ttgggccgct tggactggaa tacctccgcg
     1441 cgacggacga cccgcgaacc agatgaacga ccaacgagag agggagatcc gcgcggcact
     1501 ggatcagcat tgggcggcct ccgatgccaa cgatttcgac acggagcacc gtatctatca
     1561 cgaggacgcg gtgctggaat acccgcagtc aggcgagcgc acccgcggtc gacgcaacat
     1621 ccagagccag cgcgccagtc agccgagcca
//
LOCUS       DBNGZW010000309         4185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_640367, whole genome shotgun sequence.
ACCESSION   DBNGZW010000309 DBNGZW010000000
VERSION     DBNGZW010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4185
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_640367"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            195..710
                     /locus_tag="AC16JC_07320"
     CDS             195..710
                     /locus_tag="AC16JC_07320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5164543.1"
                     /translation="MVITDAASFLDYLSRIRGRTLAVARVIPEDRLEWRPTEGAFSFG
                     DLLRHLAAIERYMFAENARLQPARYLGHGRELADGYAAVFAYVERMHEEAVAIFAALS
                     PADLQRRCVTPGGAEIPVWKWLRAMVEHEVHHRGQIYVYLAMLGLHGPPIYGLTSEEV
                     RARSRNVKPEG"
     gene            713..1531
                     /locus_tag="AC16JC_07325"
     CDS             713..1531
                     /locus_tag="AC16JC_07325"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5164544.1"
                     /translation="MVAAFGVVLSLLLPAPTAAQAPVVAGRLEAHVLYDSSYRRPRRV
                     WVYTPPGYDSTRAAPYPLMVAFDGDEYRDTMPLPLVLDTLLAARRAPAFVAVLVDDSS
                     GAVRIADLANSARMVDFLARQLLRWVRRGWHVTRDPHRVIVTGSSAGGLAAAFVAFQR
                     PDLFGNVWSQSGAFWRGAEASNTAPWEWLTQQVAAAPRKDVRFCLDVGELEDHATLGG
                     AGPNFRDATRRLRDALVAKGYDVTYTEVPGGNHAERWWRLRLPEGIVALSAAWH"
     gene            complement(1599..2117)
                     /locus_tag="AC16JC_07330"
     CDS             complement(1599..2117)
                     /locus_tag="AC16JC_07330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164545.1"
                     /translation="MSNRWISAALAGALLGLSTPSASQTTHAMGQSPAAGAAAHRDSL
                     PVEQLALVKRATARYRDVANAVADGYADINVVIPNMGRHYLKQALMDSTFDVEHPEIL
                     VYAPDSGGTLRLVAAEYAVPTNLSTKAPEGFRGSADQWFVNEQFNIWTLHAWVWKENP
                     AGMFYPTNPNVK"
     gene            complement(2244..2897)
                     /locus_tag="AC16JC_07335"
     CDS             complement(2244..2897)
                     /locus_tag="AC16JC_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164546.1"
                     /translation="MNRISFVSAALLLVGGGLAAQQSDPDRSVAGGGVFPAGWNVRTE
                     RNAPAAQVKFVAMGGGFHATMGPAAIMWRDADVATGNYHTVATFTQTKAPAHPEAYGL
                     VIGGQHLADSAQSYTYFLVRGQGEYSIRRRAGYASRPTAVVEWTANAAVHKADSAGAA
                     TNELAVLIKDGKASFTVNGTEVYSTDAANVDASGIVGYRVNHNLDVHLSAIGIHKIQ"
     gene            3013..3435
                     /locus_tag="AC16JC_07340"
     CDS             3013..3435
                     /locus_tag="AC16JC_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR5164547.1"
                     /translation="MTEQFDLRFRTIAQTALRIAAGLTYFSHGAQKLLGWFGGVGPNG
                     GAAELMSRFGAAGVIETVCGACLVLGLFTRPLALIASGEMAVAYFWVHTARSGDLWWW
                     HNRGELVMLYSFIWLLFAAWGPGPHSLDAWIRKGRSAT"
     gene            3537..3890
                     /locus_tag="AC16JC_07345"
     CDS             3537..3890
                     /locus_tag="AC16JC_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164548.1"
                     /translation="MADCAPWDGPAVTILLTTRPADSIAVAGPHVSISVWRSTTALAG
                     QTFAWPADQQMGAATRCTTDAACEPARTARVTFAPASSDTMLGGTVELTFAGADSILR
                     GTFRARWLHTRVMCG"
     gene            3917..>4185
                     /locus_tag="AC16JC_07350"
     CDS             3917..>4185
                     /locus_tag="AC16JC_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164549.1"
                     /translation="MTRYLLPALLLTTSSAAAQSAWRLVQTTDELTAATDTRLILRED
                     AWPSEPAKAADSFRGATLVIVCGHRLPSAGGRTLLLSADQPMQPFG"
BASE COUNT          593 a         1492 c         1460 g          640 t
ORIGIN      
        1 acggggcccc catcgtcgag cgcgccatgg ccgcggagca ggcgtcggac gccgaggtgg
       61 ctcccgccgt cgatccggcg ctggccgccc ggcgcgtggc acaggccgac atggaagcga
      121 ccatgccgga gtggctcaag cgcctcaccg aggggaagtg accttctccg gctcgtaggg
      181 accggcacgc tgacatggtg atcaccgacg ccgcgtcgtt tctcgactac ctgtcccgga
      241 tccgcggccg cacgctggcc gtcgcacgcg tcatcccgga ggatcggctc gagtggcgac
      301 cgaccgaagg cgcgttctcc ttcggcgacc tgctgcgcca cctcgccgcc atcgagcggt
      361 acatgttcgc cgaaaacgct cgactccagc ccgcgcgcta cctggggcac gggcgcgaac
      421 tggccgacgg gtacgcggcg gtattcgcgt atgtcgagcg gatgcacgag gaggcggtgg
      481 cgatcttcgc cgcgctctcc cccgctgacc tgcagcgccg ctgcgtgacc ccgggcggcg
      541 ccgagatccc cgtctggaag tggctccgcg ccatggtgga gcacgaggtc caccatcgcg
      601 ggcagatcta tgtctacctc gccatgctgg gcctccacgg tcctccgatc tacggcctca
      661 cgtcggagga agtgcgtgcc cgcagccgga acgtgaaacc ggagggctga ccgtggtcgc
      721 agcctttgga gtggtactct cgctgctgct gcccgcgccc accgcggcgc aggcgccggt
      781 ggtcgccggc cggctcgagg cgcacgtgct gtacgactcg tcgtaccggc gtccgcgccg
      841 cgtctgggtg tacaccccgc cggggtacga ctcgacccgc gccgcgccgt acccgctgat
      901 ggtggcgttc gacggcgacg aataccgcga cacgatgccg ctccccctgg tcctcgacac
      961 gctgctcgcc gcccggcgcg cgcccgcgtt cgtggccgtt ctggtggacg attcgagcgg
     1021 ggcggtgcgc atcgcggacc tcgccaattc cgcccggatg gtcgacttcc tggcgcgtca
     1081 gctgctgcgc tgggtgcgcc gggggtggca cgtcacacgc gatccgcacc gcgtgatcgt
     1141 gaccggctcg agcgccggcg ggctcgcggc cgccttcgtg gcgttccagc ggcccgatct
     1201 cttcggcaac gtgtggtcgc agagcggcgc gttctggcgc ggcgcggaag cgtcgaacac
     1261 ggctccctgg gagtggctga cgcagcaggt cgcggcggcg ccacgcaagg acgtccgctt
     1321 ctgcctggac gtcggcgagc tggaagacca cgcgacgctg ggcggagcgg ggcccaattt
     1381 ccgcgacgcg acccgccgcc tccgggacgc gctcgtggcg aaagggtacg acgtgacgta
     1441 cacggaggtg cccgggggaa atcacgcgga gcgctggtgg cgcttgcggc tccccgaggg
     1501 gatcgtggcg ctgagcgccg cctggcactg aagcagccgg agcgcaggag aaaagaaaac
     1561 ggggccggag agcggctccg gccccacacg cgatcagctc acttcacgtt cggattggtc
     1621 ggatagaaca tccccgccgg gttctctttc cagacccagg cgtgcagcgt ccagatattg
     1681 aactgttcgt tcacgaacca ctgatcggcc gagccgcgga acccctcggg cgccttcgtc
     1741 gagaggttcg tgggaacggc gtactccgcc gccaccagcc gcagcgtgcc gccggagtcg
     1801 ggcgcataga cgaggatctc cggatgctcc acgtcgaacg tggagtccat cagcgcctgc
     1861 ttcaggtagt ggcgtcccat gttggggatc accacgttga tgtccgcgta cccatccgcg
     1921 acggcgttgg cgacatcgcg atagcgcgcg gtggcccgct tcacgagcgc cagctgctcg
     1981 acgggcagcg aatcgcgatg agcggccgcc cctgcggcgg gcgattgccc catggcatgg
     2041 gtcgtctggg aggcgcttgg ggtggagagc ccgagcagcg cgccggccag ggcggcggag
     2101 atccaacggt tcgacatcgg tacactcctg gaatctggaa tatgggaaag ggatccagca
     2161 caccgggcgc acgaatgcga ccggcatcgg aagaagaacc gggccgcgcg cgagatggtt
     2221 cccgcgcgcg gccccgctgc agcctactgg atcttgtgaa tcccgatcgc cgagaggtgc
     2281 acgtcgaggt tgtggttcac gcggtagccg acgatgcccg acgcgtccac gttggcggcg
     2341 tcggtcgagt acacctcggt cccgttgacc gtgaagctcg ccttgccgtc cttgatcagg
     2401 accgcgagct cgttcgtcgc cgccccggcg ctgtccgcct tgtggaccgc ggcgttcgcc
     2461 gtccattcga cgacggcggt gggccggctg gcgtaaccgg cccggcgccg gatgctgtac
     2521 tcgccctgcc cgcgcaccag gaagtaggtg tacgactgcg ccgaatcggc cagatgctgg
     2581 ccgccgatga ccagcccgta cgcctcggga tgcgccgggg ccttggtctg cgtgaacgtc
     2641 gcgacggtgt ggtagttgcc cgtggccacg tcggcatcac gccacatgat ggccgcgggc
     2701 cccatcgtgg cgtggaagcc gccccccatg gccacgaact tcacctgagc cgcgggcgca
     2761 ttgcgctcgg tccggacgtt ccagcccgcc ggaaacaccc ccccgcccgc caccgagcgg
     2821 tcgggatcgc tctgctgtgc cgccaggccg ccgccgacga gcagcagggc ggcggacacg
     2881 aaggaaatcc ggttcatcgt gcgatacctc ttgagaaagg ggtcggacgg cgcgcccccc
     2941 gccttgcgtg gggggagccg ggcggacgat tatacccgag ccgcgaccac aagttaactc
     3001 cgggaggggt ccgtgaccga gcaattcgac ctccgattcc gcaccatcgc ccagaccgcg
     3061 ctccgcatcg ccgccggcct gacatatttc tcacacggcg cccagaagct gctggggtgg
     3121 ttcggaggcg tcggccccaa cggcggggcg gcggagctca tgagccggtt cggggcggcc
     3181 ggcgtcatcg aaacggtgtg cggcgcgtgc ctggtcctcg gcctgttcac ccggccactg
     3241 gcgttgatcg cctcgggaga aatggcggtc gcctatttct gggtgcacac ggcccggagc
     3301 ggcgacctct ggtggtggca caatcgcggc gagctcgtga tgctctactc gttcatctgg
     3361 ctgctcttcg cagcctgggg accggggccg cacagcctcg acgcctggat ccgcaaggga
     3421 cggagcgcca cctgagcgga cgccgcgccg gcctcgtctt cctcctggcg ccgctgggtg
     3481 cctgcggcgc tcccactccc gccgatccgc ccgcgggata cccgtacgcc tacgcgatgg
     3541 ccgattgcgc tccctgggac ggccccgcgg tgacgatcct gctgacgacc cggcccgccg
     3601 attcaatcgc cgtcgccggg ccccacgtca gtatctcggt atggcgatcc acgacggcgt
     3661 tggccggcca gacgttcgcc tggcccgccg accaacagat gggcgccgcc acgcgctgca
     3721 ctaccgacgc ggcgtgcgag ccggcgcgca cggcgcgggt gacgttcgca ccggcgtcat
     3781 ccgacaccat gctcggcggg accgtcgagc tgacgttcgc cggcgccgat tcgatcctgc
     3841 gtgggacgtt ccgggcgcgc tggctccaca cgcgcgtgat gtgcggctga gcgcatcctc
     3901 ctcacccccg gcgcccatga ccaggtacct gctgcccgct ctgctgctca ccacgtccag
     3961 cgccgccgcc cagtccgcgt ggcggctggt gcagaccacc gacgagctca ccgccgcgac
     4021 cgacacgcgg ctcatcctgc gggaggacgc ctggcccagc gagcccgcga aggcggcgga
     4081 ttccttccgc ggcgccacgc tcgtcatcgt gtgcgggcat cggttgccca gcgccggggg
     4141 tcgaaccctg ctgctctccg cggaccagcc gatgcagccg ttcgg
//
LOCUS       DBNGZW010000310         4981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_301327, whole genome shotgun sequence.
ACCESSION   DBNGZW010000310 DBNGZW010000000
VERSION     DBNGZW010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4981
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_301327"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..721
                     /locus_tag="AC16JC_07355"
     CDS             <1..721
                     /locus_tag="AC16JC_07355"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011239838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HIR5164550.1"
                     /translation="VVLVGKGITFDSGGISIKPAANMEEMKYDMSGAAAVLGTFETLG
                     RLRPALNVVGLISSTENLPSGTAVKPGDVIKSHFGKTIEIVNTDAEGRLVLCDALSYA
                     RRYKPAAVIDAATLTGAVVIALGNQASAVMGNDDALAAEVRDAGERAGERCWPLPLWD
                     EYRDLVKSDIADVKNSGGRGAGSIAGGWFLREFVEGYPWVHIDIAGTAYTDNEAPHQV
                     KGPTAFGVRLFSEFLLRRAGT"
     gene            721..1752
                     /locus_tag="AC16JC_07360"
     CDS             721..1752
                     /locus_tag="AC16JC_07360"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR5164551.1"
                     /translation="MTRSLLLAGALLVAGGRLAAQVDTGPPRPRTKADSARADSARTA
                     ADSAARDTIERYLPTFTRAIPAGPLPRGTRWRFDGDSLVLSDITTLSDLLARIPGVYV
                     ARGGWFGAAEVPVYGGQGPRSLEVYWDGVPYLPLGRDSLWLDPARIPLGPIERIDVQI
                     LPSTLRVYLVTARQSSSVPRTGVRISTGGNSIAQYRGGFAKRWKSGIGLSVNAEYRNI
                     DGIAGSSTTPFNTVDLWLKAEYVPSPRYGVGYQVLSSSWRRGQGPQVDAWRQRRQDGV
                     LRAFYATRADGLGERVGLSVMRTQSTQDTTVPDRSLFQAVLELSETRSRASLGFTART
                     QGSGNPLQL"
     gene            1774..2511
                     /locus_tag="AC16JC_07365"
     CDS             1774..2511
                     /locus_tag="AC16JC_07365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164552.1"
                     /translation="MSFLTLAADGRRSYYRDAGHGNRAHVSAGLALPLGFSVRAEAAR
                     VEDYRAPLVADSFQWATDYAAYLRWDRRRITLEAGQVRRDAFAPLGFAAGIAPVAELG
                     ARPRSTYLSAYAAVRPLSGVELSGWYFDPVQGGGDFEPPKHARASATFYSKFWRVYKS
                     GVFSLRGEVAVESWSRSTLGGRDDAGDPIPLGPASFVTTNIQLRIADFTAYWITRNYD
                     AMRASYVTGLGYPKRAQYYGVAWFFRN"
     gene            2652..3515
                     /locus_tag="AC16JC_07370"
     CDS             2652..3515
                     /locus_tag="AC16JC_07370"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HIR5164553.1"
                     /translation="MTGFGAAEGTVAEGRLRVEIRTVNHRYFNPQFKLPYDLNGLEGD
                     LRDRLRRLLERGHVAVSARWIEQPAAAGGVALDLDRARAVVRALRQLKKTLRLKGEPD
                     LAFVARHPDVLATTSDGTVARWADVQPIVERAAADVLAMREREGSALAADLLVRVDAL
                     EAGARTTEQRAPERLAAELARLRRSVAELASGVHVDEQRLALEIALLADRVDISEELV
                     RFRTHLAAVRAALGGGDGSVGKQFGFLAQELLREVNTMGSKANDAAITQAVIAMKGEL
                     ERFREQLENLE"
     gene            3512..4180
                     /gene="gmk"
                     /locus_tag="AC16JC_07375"
     CDS             3512..4180
                     /gene="gmk"
                     /locus_tag="AC16JC_07375"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR5164554.1"
                     /translation="MTPRVVILSAPSGGGKTTISRALLARRPDVGYSVSATTRPPRPG
                     EVDGESYHFVTRAEFDRLVAAGEFLEWAEYAGNRYGTLRREVQAVRSTGKHVLLDIEV
                     LGARQVQQECPPPDSISVFVLPPDPDTWIERLVGRETEAPDVLARRLQRATKEIQESV
                     DWEHVVINDDLDAVVAEVGAIIDEDGKRAHRPPIPRLVRLVEDLVRAAVRHQPRARTN
                     DLTE"
     gene            4195..4407
                     /locus_tag="AC16JC_07380"
     CDS             4195..4407
                     /locus_tag="AC16JC_07380"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0000428 - DNA-directed RNA polymerase
                     complex [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR5164555.1"
                     /translation="MRIITPQEVANQTQNKYLGVLVAAKFARFVNEFPRDRSVEFEQK
                     LTTRALGELVQGNLKYKLVRRRRQEA"
     gene            4407..>4981
                     /locus_tag="AC16JC_07385"
     CDS             4407..>4981
                     /locus_tag="AC16JC_07385"
                     /inference="COORDINATES: protein motif:HMM:NF014494.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HIR5164556.1"
                     /translation="MGVAGRHVVLGVSGGIACYKSCTLARRLAEAGVTVDVVMTAAAT
                     EFVRPLTFEALTGRPVLTSLWERDRALAHIGLAKHPDLVVVAPATAQLLARAAMGMAD
                     DLLTAILLARPGAVLAAPAMNDAMFAHPVTQANLRALASRGWMFVGPETGALAEGPSD
                     RQGRMSEPETILAAVLRHLGADSRWTGRKVVV"
BASE COUNT          723 a         1646 c         1873 g          739 t
ORIGIN      
        1 ggtcgtactg gtcgggaagg ggatcacctt cgactccggg gggatctcga tcaagccggc
       61 cgcgaacatg gaggagatga agtacgacat gtccggggca gcggccgtgc tggggacgtt
      121 cgagacgctc gggcggctgc gcccggcgct caacgtcgtg ggcctgatct cgagtaccga
      181 gaacctcccg tccggcaccg ccgtcaagcc cggggacgtg atcaagagcc acttcggcaa
      241 gacgatcgaa atcgtgaaca ccgacgccga ggggcggctg gtgctgtgcg acgccttgtc
      301 ctatgcgcgc cgctacaagc ccgcggcggt gatcgacgcc gccacgctga ccggagcggt
      361 ggtgatcgcg ctgggcaacc aggcgagcgc cgtgatgggg aacgacgacg cgctggcggc
      421 cgaagtccgc gatgccggcg agcgggccgg ggaacggtgc tggccgctgc cgctgtggga
      481 cgaataccgc gacctcgtga agtccgacat cgccgacgtc aagaactcgg gtgggcgcgg
      541 cgcggggtcg atcgccggcg gctggttcct gcgcgaattc gtcgagggct atccctgggt
      601 gcacatcgac atcgccggga ccgcctatac cgacaacgag gccccgcacc aggtgaaggg
      661 gccgaccgcg ttcggcgtgc ggttgttcag cgagttcctg ctgcggcgcg ccgggacctg
      721 atgacccgga gcctcctcct tgcgggggcg ctcctcgtcg cgggcggccg cctcgcggcc
      781 caggtggaca ccggcccgcc ccgtcccagg accaaagcgg actcggcccg cgccgattcg
      841 gcgcgcacgg cggcggacag cgccgcgcgc gatacgatag agcgctacct ccccacgttc
      901 acgcgcgcca tcccggccgg accgctgccg cgcgggacgc gctggcgctt cgacggcgac
      961 tccctcgtcc tgtccgacat cacgacgctg tccgatctgc tggcccggat tcccggcgtg
     1021 tacgtcgccc gtggcgggtg gttcggggcg gccgaggtac cggtgtacgg cggacagggc
     1081 ccacgctccc tcgaagtcta ctgggacggc gtcccctacc tgccgctggg gcgcgactcg
     1141 ctgtggctcg acccggcgcg cattccgctc ggaccgattg agcggatcga cgtgcagatc
     1201 ctgccgtcga cgctgcgcgt gtatctcgtg accgcgcggc agagctccag cgtgccgcgc
     1261 acgggcgtgc ggatcagcac gggggggaat tcgatcgcgc agtaccgcgg cggattcgcc
     1321 aagcgctgga agtccggcat tgggctctcg gtgaacgccg agtaccgcaa tatcgacggc
     1381 atcgccggca gctccacgac acccttcaac acggtggacc tgtggctcaa ggcggagtac
     1441 gtgccgtcgc cacggtacgg cgtcggctat caggtcctga gctcctcctg gcggcggggc
     1501 cagggtccgc aggtggacgc ctggcgccag cggcgccagg acggggtgct gcgcgccttc
     1561 tacgccacgc gtgcggacgg cctgggcgag cgagtgggcc tgagcgtgat gcggacgcag
     1621 agcacccagg acaccacggt cccggatcgc tccctgtttc aggcggtgct cgaactctcc
     1681 gagacgcgct cgcgggcatc gctgggcttc accgcgcgga cgcaaggttc cgggaatccg
     1741 ctgcagctgt aaggtcgtgt cgcgtggcag ccggtgtcgt tcctgacgct cgcggccgat
     1801 gggcgccgat cctactaccg ggatgccgga cacgggaatc gcgcgcacgt gtcggccggg
     1861 ctggccctgc cgctggggtt ctccgtgcgt gcggaggccg cgcgggtgga ggactaccgg
     1921 gcgccgctgg tggccgactc gttccagtgg gccaccgact acgccgcgta ccttcgctgg
     1981 gaccggcggc gcatcacgct cgaggccggt caggtgcggc gcgatgcgtt cgcgccgctc
     2041 gggttcgctg cggggatcgc ccccgtcgcg gagctgggcg cgcggccgcg cagcacctac
     2101 ctgtccgcgt acgccgcggt acgcccgctc tcgggcgtgg agctctccgg ctggtatttc
     2161 gatccggtgc aggggggcgg cgatttcgaa ccgcccaagc acgcgcgcgc ctcggccacg
     2221 ttctactcga agttctggcg cgtgtacaag agcggcgtgt tttccctccg gggtgaggtc
     2281 gcggtcgaaa gctggagccg cagcaccctg ggcgggcggg atgatgcggg cgatccgatc
     2341 ccgctggggc ccgcgtcgtt cgtcacgacg aatatccagc tgcgcatcgc ggatttcacg
     2401 gcctactgga tcacgcgcaa ttacgacgcg atgcgcgcca gctacgtgac gggactcggc
     2461 tatcccaagc gggcgcagta ctacggagtg gcgtggttct tcaggaacta gctatcggct
     2521 atcagctatc ggctatcagc ccgtcaacag ccatatcgtg tctcttggta gctgatagcc
     2581 gatagctgat agccggtact gattgaccta agtgcttcga gcggctaagc ttaaagtctg
     2641 tgccgcggag catgaccggg ttcggcgccg ccgaagggac ggtggcggaa ggacgcctgc
     2701 gggtggagat ccgcaccgtc aatcaccgct acttcaatcc ccagttcaag ctgccctacg
     2761 acctcaacgg gctcgagggc gacctgcgcg accggttgcg gcggctgctg gagcgcgggc
     2821 acgtcgcggt gagcgcccgc tggatcgagc agcccgccgc ggcgggcggt gtggcgctcg
     2881 acctggaccg tgcgcgggcg gtggtgcgcg cgctgcggca gctgaagaag acgctgcggc
     2941 tgaaagggga gccggacctc gccttcgtgg cgcggcaccc cgacgtgctg gcgacgacca
     3001 gcgatggcac ggtggcgcgc tgggccgacg tgcagccgat cgtggagcgg gcggcggcgg
     3061 acgtgctggc gatgcgcgag cgcgaaggaa gcgcgctggc ggccgacctg ctggtgcgcg
     3121 tggacgccct ggaggcgggc gcccgcacca ccgagcaacg ggcgcccgag cggctcgcgg
     3181 ccgagctggc gcggttgcgc cggagcgtcg ccgagctggc gagcggcgtg cacgtggacg
     3241 agcagcgcct cgcgctggag atcgccctgc tcgccgaccg ggtggacatc agcgaggagc
     3301 tggtgcggtt ccgcacccac ctcgcggcgg tgcgcgcggc cctcggcggg ggcgacggct
     3361 cggtcggcaa acagttcggg tttctcgcgc aggagctgct gcgcgaggtc aacacgatgg
     3421 ggtcgaaggc gaacgatgcc gcgatcacgc aggcggtgat cgcgatgaag ggcgagctgg
     3481 agcggttccg ggagcagctg gagaatctgg agtgacgccg cgggtcgtca tcctgtccgc
     3541 cccctcgggg ggcggcaaga cgacgatttc ccgggcgttg ctcgcgcggc gccccgacgt
     3601 gggctactcg gtgtcggcga cgacgcgccc gccgcgaccg ggggaggtgg acggcgagtc
     3661 gtaccacttc gtcacgcggg cggagttcga tcgcctggtc gcggccggcg aattcctgga
     3721 gtgggcggag tacgccggga accgctacgg cacgctgcgg cgcgaggtcc aggcggtgcg
     3781 gtcgacgggg aaacacgtgc tgctggacat cgaggtgctg ggcgcgcggc aggtgcagca
     3841 ggagtgcccg ccgcccgatt cgatcagcgt gttcgtgctc ccgccggacc cggacacctg
     3901 gatcgagcgc ctcgtcgggc gggagaccga ggctccggac gtgctggcgc gacggctgca
     3961 gcgcgcgacg aaggagattc aggagtcggt ggactgggag cacgtcgtga tcaacgacga
     4021 tctcgacgcc gtggtcgccg aggtgggggc catcatcgac gaagacggga agcgggcgca
     4081 ccgtccgccg attccgcgcc tggtgcggct ggtcgaggac ctggtgcgcg cggccgtgcg
     4141 gcatcagccg cgggcgagaa ccaacgactt aaccgagtag gggaagagga cccgatgcga
     4201 atcatcactc cgcaggaagt cgcgaatcag acccagaaca agtacctggg cgtcctggtg
     4261 gccgcgaagt tcgcgcgctt cgtcaacgag tttccccgcg atcgcagcgt ggagttcgag
     4321 cagaaactga cgacccgcgc gctgggcgag ctggtccagg ggaacctgaa gtacaagctg
     4381 gtgcggcggc ggcggcagga ggcctgatgg gcgtcgccgg ccgtcacgtc gtgcttggcg
     4441 tgtcgggcgg catcgcctgc tacaagagct gcacgctggc ccggcggctc gccgaggccg
     4501 gcgtgacggt ggacgtggtg atgaccgccg cggccaccga gttcgtccgt ccgctgacct
     4561 tcgaggcgct caccgggcgg ccggtcctca cctccctctg ggagcgggac cgcgcgctgg
     4621 cccacatcgg cctcgccaag catccggatc tggtggtcgt ggcccccgcg acggcgcagc
     4681 tgctggcccg cgcggcgatg ggcatggccg acgatctgct caccgcgatc ctgctggcgc
     4741 gtcccggcgc cgtgctcgca gcccccgcca tgaacgacgc catgttcgcc caccccgtga
     4801 cgcaggccaa tcttcgcgcc ctcgcctcga ggggctggat gttcgtcggt cccgagaccg
     4861 gggcgctggc cgagggcccg agcgaccggc agggacgaat gagcgagccg gaaacgatcc
     4921 tcgcggcggt gctgcgtcat ctcggcgcgg attcgcgctg gacgggacgc aaggtggtcg
     4981 t
//
LOCUS       DBNGZW010000311         3588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_702872, whole genome shotgun sequence.
ACCESSION   DBNGZW010000311 DBNGZW010000000
VERSION     DBNGZW010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_702872"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1360
                     /locus_tag="AC16JC_07390"
     CDS             <1..1360
                     /locus_tag="AC16JC_07390"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR5164557.1"
                     /translation="QWYPRLAVYDDVRGWNTDQYIGSSEFYLEYGDFDVTLTLPAGFT
                     VAATGVLRNPLEVLPAKIRARLAAAAAADTIVHIIPAEEVGTPGLLPAGAGPTRAWHF
                     TATNVRDFAWGTAPNFLWDATSWDGILMQALYPPTVADVWKTAADMNRHAVMIHSRWL
                     HYPWPTAISVEGPVGGMEYPMMTFDNARDEKELYYTIAHEHGHEWFPMMVGSQERLYP
                     WMDEGFNTFIDWFSFRDRYPADTTRIQTLEFGSMRAYQDFLRTYRGVETPIMESQDRT
                     PNGLMSGWNAYGKPAVALHFLREQVVDSNAFDDAFREYVRRWAFKHPQPADFFRTMNN
                     ALGEDLSWFWRGWFLRSDHLDQAVDSVVQLDTAGQVLTRVFLSSRREMVAPVELGVTA
                     GDGSTRTVKLPVETWLRGSRAVWALLTPGATKPVKIEIDPRGVYPDVDRSNNVWTSQP
                     GP"
     gene            1447..3063
                     /locus_tag="AC16JC_07395"
     CDS             1447..3063
                     /locus_tag="AC16JC_07395"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR5164558.1"
                     /translation="MRPSVLALLPLLAAGTVDAQSTLMWRDTLPGEVSGFFVSPLGNL
                     IVATPGAFSALDPTSGGLMWRVSLQSGHEITALVDTPFGVLETEDSIALLDLTTGEQR
                     WDESVLGSGRLYGYLAARSLLLCLMGEADSASLIAVDLGSGQLRWRHDHPFTAAPKHY
                     RTLVSQRKVFNQLAGEEPPLWVNDTTFILYVSADGPVLVDANTGAFLWQASALQGERP
                     PAARDAYPPMLQHDSLILVPYGKRLHAIRLRDGSAAWAQPAEFPSRIAQLELTPNGVL
                     VRGARREGDAPKDAPFIAFVNPATGTPVWPEMWKRGYSFLAAANHDDPPPNTSPFVPR
                     GNRVYFAWKGDLYALTLGDGEATKLGKLRFKGGEAPELVENRADGILFFSAQNMLLAD
                     TTGRSLIQRYFPAPPVGWLAHVAKVAVLVTLDVAQAALAQSQANRTGVPQTYYPVYSD
                     NPFLRARYAAAGVADDYSYVLTTDKDSTGQSRSVLIRVPKDRDATEGRVWLSEKHPPF
                     RVDPFTASVYVQTGPNEITAFRFPVVTGGP"
     gene            complement(3113..>3588)
                     /locus_tag="AC16JC_07400"
     CDS             complement(3113..>3588)
                     /locus_tag="AC16JC_07400"
                     /inference="COORDINATES: protein motif:HMM:NF020483.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1854 domain-containing protein"
                     /protein_id="HIR5164559.1"
                     /translation="STQPGTALRLESRADGQIWATRDGVERAVSVRRCFPWSEPGRFV
                     SLRDHEDEEFALVADAKVLDRGSRRVLEEALVEAGFVLQVTQVLEIEEEVEIRRWHVR
                     TRQGPRRFVTRLDDWPRALPGGGLLLRDVAGDLYHLGDPAGMDRESRELLWAFVD"
BASE COUNT          585 a         1270 c         1165 g          568 t
ORIGIN      
        1 ccagtggtat ccacgcctgg cggtgtacga cgacgtacgc ggctggaaca ccgatcagta
       61 tatcggctcg agcgagttct acctcgagta cggggacttc gacgtcaccc tcaccctgcc
      121 cgcgggcttc acggtggccg ccaccggcgt gctgcgcaac ccgctcgagg tgctgccggc
      181 gaagatccgc gcccgtctgg cggccgcggc tgccgccgac acgatcgtgc acattatccc
      241 ggccgaggaa gtcgggaccc cggggctgct tccggccggc gccggtccga cgcgcgcctg
      301 gcacttcacc gcgaccaacg tgcgcgactt cgcctggggc acggcgccga acttcctgtg
      361 ggacgccacc agctgggacg gcatcctgat gcaggcgctg tatcccccga ccgtcgcaga
      421 cgtgtggaag acggcggcgg acatgaaccg ccacgccgtg atgatccact cccgctggct
      481 gcactacccg tggcccaccg ccatcagcgt ggaaggtccg gtcggcggca tggaataccc
      541 catgatgacc ttcgacaacg cgcgggacga gaaggagctg tactacacca tcgcccacga
      601 gcacgggcac gaatggttcc ccatgatggt gggctcccag gagcggctct acccgtggat
      661 ggacgagggc ttcaacacgt tcatcgactg gttcagcttc cgcgaccgtt atcccgccga
      721 cacgacgcgc atccagacgc tggaattcgg ctcgatgcgc gcctaccagg acttcctgcg
      781 cacctatcgc ggcgtcgaga ccccgatcat ggagtcgcag gaccgcacgc ccaacgggct
      841 catgagcggc tggaacgcct acggcaagcc ggcggtggcg ctccatttcc tgcgcgagca
      901 ggtcgtggac tcgaacgcct tcgacgacgc gttccgggag tacgtgcgcc gctgggcgtt
      961 caagcacccc cagcccgccg acttcttccg cacgatgaac aatgccctgg gggaagatct
     1021 gtcgtggttc tggcgcggct ggttcctgcg ctccgaccat ctggaccagg cggtggattc
     1081 ggtcgtgcag ctggacaccg ccggtcaggt cctgacgcgc gtgtttctct ccagccgccg
     1141 cgagatggtg gccccggtcg agctgggtgt caccgccggt gatggctcca cgcggaccgt
     1201 gaagctgccg gtcgagacct ggttacgggg gtcgcgggcg gtatgggcac tgctcacgcc
     1261 cggggcgacg aagccggtca agatcgagat cgatccgcgc ggcgtgtacc cggacgtgga
     1321 ccggtccaac aacgtctgga catcgcagcc cgggccataa cgccgttgga cctgattggg
     1381 ggcactggca ggcacgaccg ggcgggatac gttcgttgag cagaatcctc gcgccgtgga
     1441 ggtcccatgc gaccgtccgt ccttgcgctg ctgccgctcc ttgccgccgg caccgtcgac
     1501 gcgcagagca ccctcatgtg gcgggacacc ttgcccgggg aagtctccgg gttcttcgtc
     1561 tccccgctcg gcaacctcat cgttgccacg cccggcgcgt tcagcgccct cgatccgacg
     1621 agcggcggac tcatgtggcg ggtgtccctg cagagcggcc atgagatcac tgcgctcgtc
     1681 gacaccccct tcggcgttct ggagaccgag gactcgatcg cgctgctcga tctcaccacg
     1741 ggtgagcaac gatgggacga gagcgtgctg ggatcgggcc ggctctacgg ctatctggcc
     1801 gccaggagcc tgttgctctg cctcatggga gaggccgact cggcgtcgct catcgcggta
     1861 gatctcgggt ccggccagct ccgctggcgg cacgaccatc cattcaccgc cgcgccaaag
     1921 cattaccgaa cactcgtcag ccagcggaaa gtgttcaacc aactggcggg cgaagagcca
     1981 ccgctctggg tgaacgacac caccttcatc ctgtacgtga gcgcggacgg accggtgctc
     2041 gtcgacgcca acaccggcgc attcctctgg caagcctcgg ccctgcaggg cgagcgaccg
     2101 cccgccgcgc gcgacgcata cccgccgatg ctgcagcacg attcgctcat cctggtgccc
     2161 tatgggaagc ggttgcatgc gatccggctg cgggacggca gcgcggcctg ggcccagcca
     2221 gcggagttcc ccagtcgaat cgcgcaactc gagctgaccc cgaacggcgt gctcgtgcgg
     2281 ggagcccgac gcgagggcga cgcgccgaaa gacgcaccgt tcatcgcgtt cgtgaacccg
     2341 gcaaccggca cgcccgtgtg gcccgagatg tggaagcggg gctacagctt cttggcggcg
     2401 gccaaccacg acgatccgcc gcccaacacg tctccgttcg ttccgcgtgg aaaccgcgtc
     2461 tatttcgcgt ggaagggaga cctctacgcc ctgacgctgg gcgacggcga ggccaccaaa
     2521 ctcggtaagc tacggttcaa gggtggagag gctccggagc tcgtcgagaa ccgggcggac
     2581 ggaatcctgt tcttctccgc gcagaatatg ctcctggcgg acaccacggg ccgctccctg
     2641 atccagcgat acttcccggc gccccccgtc ggatggctcg cccatgtggc caaggtggcg
     2701 gtgttggtca cgctcgatgt tgcccaggct gcgctcgcgc agtcgcaggc gaaccgtacc
     2761 ggtgtcccgc agacgtatta tcctgtgtac tccgacaacc cgttcctccg agcgcggtac
     2821 gcggccgccg gggtcgccga cgattactcc tacgtgctca cgaccgacaa ggactcgact
     2881 gggcagagcc gctcggtgct gatccgagtc cccaaggacc gagacgctac cgaaggacgc
     2941 gtgtggctga gcgagaagca tcccccattc cgggtggatc cgttcacggc gtcggtgtac
     3001 gtccagaccg ggccgaatga gatcacggcg ttccgctttc cggtcgtgac gggaggcccc
     3061 tgagtacggc gagggcggtg gccggcaggg cacgccgacc aaccgtccgc cgtcaatcca
     3121 cgaacgccca cagcagctcg cggctctccc ggtccatccc cgccggatcg ccgagatgat
     3181 acagatcgcc ggccacgtcg cggagcagca gccctccgcc cggcagggcg cgtggccaat
     3241 cgtcgagccg cgtcacgaac cgccgtggtc cctgccgggt gcgcacgtgc cagcgccgga
     3301 tctcgacttc ctcctcgatc tccagcacct gcgtcacctg caggacgaac ccggcctcga
     3361 ccagcgcctc ctccaacacc cggcgcgacc cgcgatcgag gaccttcgcg tcggcgacga
     3421 gggcgaattc ctcgtcctcg tgatcgcgca gcgacacgaa ccgcccgggc tcggaccagg
     3481 ggaaacaccg tctcacggac acggcacgct ccacgccgtc ccgcgtggcc cagatctggc
     3541 cgtcggcacg gctctccagc ctgagtgccg tgcccggttg ggtcgacg
//
LOCUS       DBNGZW010000312         1722 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1323510, whole genome shotgun sequence.
ACCESSION   DBNGZW010000312 DBNGZW010000000
VERSION     DBNGZW010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1722)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1722)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1722
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1323510"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="AC16JC_07405"
     CDS             complement(<1..483)
                     /locus_tag="AC16JC_07405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164560.1"
                     /translation="MRPALFSALIAVASPLSAQSLLFRSPNLSGTWVPDGGVVQFNFL
                     HRFYVSPPNGKSVTNFPTFTLAVGLPAHTAVGLHYATRSEIATTFTNEVEVFARWRHA
                     AGPVMLSLTPAYNTAAKSFDGEVGVDWSRGPVTLLAAARGMAHAYRVDTARLALAGGA
                     V"
     gene            complement(618..1121)
                     /locus_tag="AC16JC_07410"
     CDS             complement(618..1121)
                     /locus_tag="AC16JC_07410"
                     /EC_number="3.5.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF025793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004132 - dCMP deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006220 - pyrimidine nucleotide metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009972 - obsolete cytidine deamination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxycytidylate deaminase"
                     /protein_id="HIR5164561.1"
                     /translation="MSLPKDGVAANTHRLNLLPDDAGAPPVERIPLYEVYMRMAEELA
                     KRSTCLRLQVGTVVTDQLLENVLAIGYNGNAKGLANRCDSSVPGNCGCIHSEMNALVK
                     APGSVRDKVVFVSASPCVMCAKLMINSGVTHVFYRKAYRDPSGIEVLGQGGVTAVLYD
                     RWVKEWR"
     gene            complement(1108..>1722)
                     /locus_tag="AC16JC_07415"
     CDS             complement(1108..>1722)
                     /locus_tag="AC16JC_07415"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0043137 - DNA replication, removal of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HIR5164562.1"
                     /translation="YRSPGRYSALMATAAIPVLVHADESCLGNDRTKPSPGGNAALVE
                     APAGDSVARWDFYECSPQTTNNRMALAGAIAALEWIRRQWQHAQVTWVSDSEYLVKGM
                     REWLPAWKARGWKRKTGAIENLELWQKLDQAMAAHAVVWKWVRGHADHPKNEYANYLA
                     QRAAERQERSNGLVASGFDTWLAQQRARGKYQSYDPDREVHEPA"
BASE COUNT          269 a          572 c          593 g          288 t
ORIGIN      
        1 caccgcaccg ccggccaacg ccaggcgcgc cgtgtccacg cggtaggcat gcgccatgcc
       61 gcgcgcagcc gccagcaggg tcaccggccc acggctccag tcgaccccca cttcgccgtc
      121 gaagctcttg gccgcggtgt tgtatgcggg ggtgagcgac agcatcaccg gaccggccgc
      181 gtgccgccac cgggcgaaca cttcgacttc gttggtgaac gtcgtcgcga tttccgatcg
      241 cgtggcgtag tgcagcccca cggccgtgtg tgccgggagg ccgaccgcca gggtgaacgt
      301 cgggaagttg gtcaccgact tgccgttcgg cggtgagacg tagaaccgat gcaggaagtt
      361 gaattgcacg acgcccccgt ccggtaccca ggtgccgctc aggttgggcg agcggaagag
      421 cagggactgc gccgacagcg gggacgcgac cgcgatcagg gcgctgaaca gggcagggcg
      481 catatgaggc ctcccggggg ctggtgagag ccggtgctgg ttgcggattg gcgattgcgg
      541 attgcggatt ggatcgtatg agtcgagagc ctacaccgca aatccgcaat ccgcaatcca
      601 aaatccgcaa tcgtgagtca gcgccactcc ttcacccagc ggtcgtacag caccgccgtg
      661 acgccgccct ggcccagcac ctcgatgccg gacggatcgc ggtacgcctt tctatagaag
      721 acgtgcgtca cgccggaatt gatcatcagc ttggcgcaca tcacgcaggg cgacgcgctc
      781 acgaacacga ccttgtcgcg cacgctgccc ggcgccttga ccagggcgtt catctcggaa
      841 tggatgcagc cgcaattccc cgggaccgag ctgtcgcacc ggttggcgag ccccttggcg
      901 ttgccgttgt agccgatcgc gaggacgttt tccagcagct gatcggtgac gaccgtgccg
      961 acctgcaggc gcaggcaggt cgagcgtttg gccagctcct cggccatccg catgtacacc
     1021 tcgtagagcg ggatgcgctc caccggcggc gcaccggcgt cgtcgggcag gaggttgagc
     1081 cgatgagtgt tggcggcgac gccgtcctta ggcaggctca tggacttccc ggtcggggtc
     1141 gtaggactgg tacttgccgc gggcgcgctg ctgggcgagc caggtgtcga agccggacgc
     1201 gacgagtccg ttggagcgct cctgccgctc ggcggcacgc tgggccagat agttggcgta
     1261 ctcgttcttg gggtgatcgg cgtgcccgcg cacccacttc cagacgacgg cgtgggcggc
     1321 catggcctgg tccagcttct gccacaactc gagattctcg atcgcgcccg tcttccgctt
     1381 ccagccgcgc gccttccagg ctggcagcca ttcgcgcatg cccttcacca gatactccga
     1441 gtcggacacc caggtgacct gcgcgtgctg ccactggcgc cggatccatt cgagtgcggc
     1501 gatggcgccc gccagcgcca tgcggttgtt ggtcgtctgc ggcgagcact cgtagaagtc
     1561 ccagcgggcg acgctgtcgc ccgccggcgc ctccaccagc gccgcgttgc cgccgggact
     1621 gggcttggtg cggtcgttgc cgaggcagct ctcgtcggcg tggacgagga ccggaatcgc
     1681 ggccgtcgcc atcagggctg aatagcgacc gggagatcgg ta
//
LOCUS       DBNGZW010000313         4096 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_781304, whole genome shotgun sequence.
ACCESSION   DBNGZW010000313 DBNGZW010000000
VERSION     DBNGZW010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4096)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_781304"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            1..1159
                     /gene="aroC"
                     /locus_tag="AC16JC_07420"
                     /pseudo
     CDS             1..1159
                     /gene="aroC"
                     /locus_tag="AC16JC_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011905738.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
     gene            1156..1674
                     /locus_tag="AC16JC_07425"
     CDS             1156..1674
                     /locus_tag="AC16JC_07425"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR5164563.1"
                     /translation="MMRHVVLLGLPGAGKSTVGRLVAEAVRAPCQDIDAIVTRKEGRP
                     IAMIFAERGEAAFRELERREMEGALAGPPAVIIPGGGWAAQPGALDSARGRALLVYLK
                     TRPDTATARAEPDGNRPLLSGEDPPLRMQQLLRERERFYLEADAQVETDRKSAAKVAD
                     EVVELARRRAGW"
     gene            1735..>4096
                     /gene="topA"
                     /locus_tag="AC16JC_07430"
     CDS             1735..>4096
                     /gene="topA"
                     /locus_tag="AC16JC_07430"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR5164564.1"
                     /translation="MKQLPADSSPKPARRRRVAATAAAADVPPPKDTDLEHEGGRGGA
                     LVIVESPTKAKTIGKYLGRGYTVKATVGHVRDLPPRKLGVDVEHGFTPEYVTIKEKAK
                     TLAEIKKAAKGASRVLIATDPDREGEAIAWHVASQLGNGGTVHRVLFHEITKDAVSQA
                     IANPQDIDQKKVDAQQARRILDRLVGYKASPLLWKSIKSGLSAGRVQTVALRLICERE
                     EEIRRFVPQEYWTIEAELEKDGQAFQARLHKLDGRKPEIRTGAAAQEIVDAAAHLPFV
                     VTDVTRKEKRRNPPAPFITSTLQQEAAKQLGFSAKRTMRAAQALYEGIEVGEEGPVGL
                     ITYMRTDSVRVADSAIAQARDFIAANYDARYLPASPNVHAGKKSASRVQGAHEAIRPT
                     EVARRPEAMKQYLESDLFKVYQLIWRRFVASQMTPELYELTTVDFDLGRFLFRATGSV
                     PLFDGYRVLFHEGHEPEEAKSGDDFGPIPPLAKGDQPTLRQVVPSQHFTEPPPRYSQA
                     SLVKALEEKGIGRPSTYASIIGTLQDRWYATTKERRFSPTPLGEKVWKVMQRCFPEVF
                     EVGFTAQMETELDKVEEGDLGWAHVLGDFWGPFNEALASVDIPSILYDVHDLSKLHEE
                     RCPTCGSPLEVRSGRFGPFIACAKYPDDCRFTKPIGRDKVPDKPSNEICKECGAPMVI
                     KTGRFGEFLACTRFPKCKHTRPVPLGITCPKCGQGDIAERRTRKGRNFFGCTRYPDCD
                     YSVWDRPYATEICPSCGFAGMTKKETKDGRVTRKCLKCGFEDAGAEI"
BASE COUNT          706 a         1377 c         1472 g          541 t
ORIGIN      
        1 ggcgggggaa agccacggcc gcggactggt ggccgtgctg gaggggatgc cggccggcct
       61 gccgctggac gcggccgcgg tgaacgccga gctcaagcgg cggatggccg gctacgggcg
      121 cggcgcccgg atgaagatcg aggccgatca gatcgaatgg ctgtcgggcg tgcgcgccgg
      181 cgaaacgatc ggcgcaccca tcgccatgct gatctggaac cgcgattggg agcactggca
      241 ggacgtgatg gccccctagg ccggtccggg cgatgcgcca aggcgccggc aggtcacccg
      301 cccgcgcccg ggacatgccg acctgaccgg cgcgctgaag tacgatcgcg aggacgcgcg
      361 ggacatcctc gagcgcgcca gcgcgcggga gacggcggcg cgcacggcgt gcggcgccgt
      421 gggcaagcgg ctgctggggg agttcggcat cgaggtcgcg agtcacgtgg tggagctcgg
      481 gggggtcacg gcgacgccgc gctcgcccct gcccacgtcc ctcaacgccg ccgccgatgc
      541 gagcccggta cggtgtctcg acccggaggc cgagaaggag atgatcgcgc gcatcgacgc
      601 cgccaaggcg gccggtgata cattgggcgg tgtggtggaa gtcgtggcct tcggactccc
      661 cgtggggctc ggttcgcatg cgtcctggga tcgcaagctg gatggccgcc tggcacaggc
      721 gatcatgtcg atcccggcgg tgaagggcgt ggcgctgggc ctggggttcg aggcggcccg
      781 gcggcgcggc tcggaagtgc acgacgagat cctccccggg ttccaccgcg ccaccaatcg
      841 cgccggcggc accgagggag gcatgtccac cggcgagccg ctcgtgctgc acgccgcgat
      901 gaagccgatc tcgacgctca tgacgccgct ggccactgtc gatctggcgt cgggcacggc
      961 ggcgcgggcg caatcggaac ggtccgatgt aaccgcggtc cccgcgatgg gggtgatcgc
     1021 ggaggccatg acggccctgg tgctgacgga ggcgatgatc gagaagttcg gcggggactc
     1081 cctcggcgag atgcggcgca acgtggacgg ctaccttgcc cgggtcaggg cccgcacgcc
     1141 gcggggagcg gcctcatgat gcgccacgtg gtgctgctcg ggctgccggg agccggcaag
     1201 tcgaccgtgg gacgtctcgt cgccgaagcc gtgcgggcgc cgtgccagga catcgacgcc
     1261 atcgtgaccc gtaaggaggg ccggcccatc gcgatgatct tcgccgagcg cggcgaagcc
     1321 gcgttccgag agctcgagcg ccgggaaatg gaaggcgcgc tcgccggtcc gccggccgtg
     1381 atcatccccg gcggcggatg ggccgcgcag ccgggtgcgc tcgactcggc gcggggccgc
     1441 gcgttgctcg tctacctcaa gacgcgtccc gacacggcca cggcgcgcgc cgaaccggac
     1501 gggaaccgtc cgctgttgag tggggaggac cccccgctgc ggatgcagca gctgctccgc
     1561 gagcgcgagc ggttctatct cgaagcggat gcgcaagttg agaccgaccg gaagagcgcg
     1621 gcgaaagtcg ctgacgaggt ggtcgaactt gcgcggagga gagcaggatg gtaaagatgc
     1681 gtgcctctga aattgaggaa gggttgatca gtggctggta agaataagaa gaccgtgaag
     1741 cagcttcctg cagactcgag ccccaagccg gcgcgacgcc ggcgtgtcgc cgctacggcc
     1801 gcggcggccg acgttcctcc tcccaaggat acggacctgg agcacgaggg gggacgcggg
     1861 ggcgcgctcg tgatcgtcga gtcgcccacc aaggcgaaga cgatcggcaa gtacctcggt
     1921 cgcggctaca cggtgaaagc gaccgtgggg cacgtccgcg acctgccgcc ccgcaagctc
     1981 ggcgtggatg tggagcacgg cttcaccccc gagtacgtca cgatcaaaga gaaggccaag
     2041 accctggccg agatcaagaa ggccgccaag ggagcgagcc gggtgctgat cgcgaccgac
     2101 cccgaccgcg agggcgaggc gatcgcctgg cacgtggcct cccagctcgg caacggcggc
     2161 acggtccacc gggtgctgtt ccacgagatc accaaggacg ccgtgtcgca ggcgatcgcc
     2221 aatccgcagg acatcgacca gaagaaggtg gacgcgcagc aggcgcggcg cattctcgac
     2281 cgcctggtgg gctacaaggc gagcccgctc ctctggaaga gcatcaagtc cgggctctcc
     2341 gccggccgcg tccagacggt cgcgctgcgg ctcatctgcg agcgggaaga ggaaatccgc
     2401 cgcttcgtgc cgcaggaata ctggaccatc gaggcggaac tcgagaagga cggccaggcg
     2461 ttccaggccc ggctccacaa gctggacggc cgcaagccgg agatccggac cggagccgcc
     2521 gcgcaggaga tcgtcgacgc ggccgcgcac ctcccgttcg tcgtcaccga cgtcacgcgc
     2581 aaggagaagc ggcgcaatcc gccggctccc ttcatcacca gcacgctgca gcaggaagcc
     2641 gccaagcagc tcggcttctc ggccaagcgg accatgcggg ccgcgcaggc gctgtacgag
     2701 ggcatcgagg tcggggagga agggccggtc gggctcatca cctacatgcg caccgattcc
     2761 gtgcgcgtcg ccgacagcgc catcgcccag gcacgcgact tcatcgccgc caattacgac
     2821 gcccgctacc tgcccgccag ccccaacgtt catgccggca agaagagcgc gtcgcgggtg
     2881 cagggcgcgc acgaggccat ccgccccacc gaggtcgcgc ggcggccgga ggcgatgaag
     2941 caatacctcg agtccgatct cttcaaggtg taccagctca tctggcggcg gttcgtcgcg
     3001 tcgcagatga cgcccgagct gtacgagctc acgacggtgg acttcgatct gggccgcttc
     3061 ctgttccgcg ccaccggctc cgtgccgctg ttcgacgggt accgcgtcct ctttcacgag
     3121 ggccacgagc cggaagaggc caagagcggc gacgacttcg gtcccatccc gcccctcgcc
     3181 aagggcgacc aaccgacgct gcgccaggtc gtcccctcgc agcacttcac cgagccgccg
     3241 ccccgctaca gccaggccag cctggtcaag gcgctggagg agaaaggcat cggccggccg
     3301 tccacctacg ccagcatcat cggcacgctg caggaccgct ggtacgccac caccaaggag
     3361 cgccgcttct cgcccacgcc gctgggcgaa aaggtgtgga aggtgatgca gcgctgcttt
     3421 ccggaggtgt tcgaggtcgg gttcaccgcc cagatggaaa ccgagctcga caaggtggag
     3481 gaaggcgacc tcgggtgggc acacgtcctg ggcgacttct ggggtccgtt caacgaggcg
     3541 ctcgccagcg tggacatccc gtccatcctg tacgacgtcc acgatctgtc caagctccac
     3601 gaggagcgct gccccacctg cggcagcccg ctcgaggtgc gcagcgggcg cttcggaccg
     3661 ttcatcgcct gcgccaagta tccggacgac tgccgcttca ccaagccgat cgggcgcgac
     3721 aaggtcccgg acaagccgag caacgagatc tgcaaggaat gcggcgcccc gatggtgatc
     3781 aagacggggc gcttcgggga gttcctcgcc tgcacgcgct tccccaagtg caagcacacc
     3841 cggcccgttc ccctcgggat cacgtgtccc aaatgcggcc agggtgacat cgccgagcgc
     3901 cgcacccgca agggacggaa cttcttcggc tgcacgcgct acccggattg cgactactcc
     3961 gtgtgggacc gcccgtacgc gacggagatc tgcccgagct gcgggttcgc cggcatgacg
     4021 aagaaggaga ccaaggacgg gagagtcacg cgcaagtgcc tgaagtgcgg gttcgaggac
     4081 gccggagcgg agatcg
//
LOCUS       DBNGZW010000314         5408 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1663761, whole genome shotgun sequence.
ACCESSION   DBNGZW010000314 DBNGZW010000000
VERSION     DBNGZW010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5408)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5408)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5408
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1663761"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..962)
                     /locus_tag="AC16JC_07435"
     CDS             complement(<1..962)
                     /locus_tag="AC16JC_07435"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5164565.1"
                     /translation="MRVLITGSTGFIGGHLIRRLAGTPHELRCLVRDPGGRGARALAC
                     RGAEVVAGDVTDPDAVRRAVAGCDAVVHLANIYSFWERDPRVFDAVNVRGTGSVMDAA
                     LAAGVSRVVHLSTVEALESAPRDNRYARSRYAGEQRAWAAARRGLPLVVLHPGAVLGR
                     GDRKPTGRYIRDLARRRLPFALCTRTVLTLVHVRDVVEAIVRALESPAVVGKRYIVGV
                     HQVSIGELNRMVSEIAGVPPPDWRLPDVLVLPVAALLTGLARITGRPPAWGLSLDMAR
                     LLRRGFRYDGSAAARELGFCYTPIRDAVAEMLGVGADRGPGFGVR"
     gene            complement(1293..2915)
                     /locus_tag="AC16JC_07440"
     CDS             complement(1293..2915)
                     /locus_tag="AC16JC_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164566.1"
                     /translation="MRPLRIADWGLRIEQLFPIHNPQSAIPNGLQSAIRNRQSAILIA
                     LLALAAPLCGQTASPYVPLNYWGNPYVEHFIAAGKMVDPSPLTRPVRADDLVRALEAM
                     DSTRLGSGEWGVVRSLLHDLRRHEQGPSGRVDADLGVAAATHSLRDPLELFRGAPPRG
                     DTVSRGFLSGGLALTLALGPVVAVTHPLFDTRLKYDRDWFGRKDRAIAGRMAESYLSA
                     QWGFGELFFGRMDRNWGPSVVQGLLLSDDPYGLDHLGITLGTAAVQLQAIVTQLDSRT
                     DSLGRINRRYMVQHRLWLRPPGRWTAALWEGSVLSGADRDLEPWYLNILNLGFLEQLN
                     TGTNVNSFVGLDLERRGGVTAYAQLMLDDIQVDRKTLTDQKPTSWGLTVGAKGGVAGA
                     AWTLFYTQVANLTYRNEDNLQVPLYHGLGTGRNFADYDQATARLSVVGPGGVLLSPEV
                     TLLRQGQGDPRLAHPAPPDYPATASLFQGTVEHTVRLALGVHWSAGPVTLTADGGAHR
                     IVNEGHVKGSSETRFVGSVGLSWSLHRAGQIP"
     gene            complement(2912..4894)
                     /locus_tag="AC16JC_07445"
     CDS             complement(2912..4894)
                     /locus_tag="AC16JC_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5164567.1"
                     /translation="MRIRNWSSVLLLLLAAAGPVAAQVSQSGPLRKTDLIRLLTSGRP
                     PGNVALMVRRRCIAFTPSPRDRFDLAEAGADKEVLDAIDGCASRATRPGGSGVPRIVA
                     PARLRATAGSEVALTVRLLRGGVPLGGKSLQLRGATAIPGGAAQEPGAVTDARGTATF
                     RVLAGMQAGTYTLEVIAVGDMPAQATLQLVTVAPSLLADVLPPVVVVREGSRFPAVVR
                     VVVRGSGGVPVAALPVELRGLGARLGPLPPVNTDGTGTALLPIPAGALHSGGTIGVFA
                     RGTQLASITARIETPAISDDRTQFIAGTGQRGSVHTTLRQRLLLEVRDTAGAPVTGYA
                     VRFTPVNAALSDTETVTDSSGVARTTVTLGDRAGPVVITATAGRVSRTTNLFATAGPA
                     QTLVVEQGGRPVDSLVLATSVTATVRMVARDAWGNEAILLGQTATVKGTAAGLHNLRG
                     VATAPGTLVLEPRRNGRATLEVSAAGEGQRLSARVPLQVTLPEAPGGWVYGARAGGAA
                     FGYSFDGAPAVNGRPGFRTELMVGRDVAPGLRVLGGLGLGVVRAHTGSASLAVGLIQG
                     LVRGEYELTRNSTVVPVVTLGGGAYRLKSTDQSSIVYHTSLFWLVGAGIDYPLGNRVI
                     GGLRLERQQLYEVTSTYTNGSVGALTLLEVGVRVMP"
     gene            complement(5107..5286)
                     /locus_tag="AC16JC_07450"
     CDS             complement(5107..5286)
                     /locus_tag="AC16JC_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164568.1"
                     /translation="MCVSAPQAAPPGAAPRSPGLPRWLHWALGALAVAGAAVAVSRPT
                     RGVAERVCGVVLVPK"
BASE COUNT          809 a         2021 c         1902 g          676 t
ORIGIN      
        1 cggacaccga accccggtcc ccggtctgct cccaccccaa gcatctccgc caccgcatcc
       61 cgaatcggtg tgtagcagaa ccccagctcg cgcgccgccg cgctgccgtc gtagcggaag
      121 ccgcgccgca gcagccgcgc catgtcgagc gacagccccc acgcgggtgg ccggccggtg
      181 atgcgcgcca ggccggtgag cagcgcggcc acggggagca ccagcacgtc cggcaggcgc
      241 cagtcgggcg gcggcacgcc cgcgatctcg ctcaccatgc ggttcagctc gccgatcgac
      301 acctggtgca cgcccacgat gtagcgcttc cccaccaccg cggggctctc gagcgcccgc
      361 acgatcgcct ccaccacgtc gcgcacgtgc accagggtga gcacggtgcg ggtgcacagc
      421 gcgaacggca ggcgccgccg cgcgaggtcg cggatgtagc gccccgtggg tttgcggtcg
      481 ccgcgcccca gcaccgcccc gggatggagc accacgagcg gcaggccgcg ccgggccgcg
      541 gcccaggcgc gctgctcccc cgcgtacctg ctgcgcgcgt agcggttgtc gcgcggcgcg
      601 ctctccagcg cctcgaccgt ggagaggtgc accacccggg acacgcccgc ggcgagcgcc
      661 gcgtccatca cggaccccgt gccccggacg ttcaccgcgt cgaacacgcg cggatcgcgc
      721 tcccagaagg agtagatgtt ggcgagatgg acgaccgcgt cgcacccggc cacggcgcgc
      781 cgtacggcgt ccgggtcggt gacgtccccc gccacgacct ccgcgccgcg gcacgccagc
      841 gcgcgcgccc cccgcccccc gggatcgcgc acgagacagc gcaactcgtg cggcgtgccg
      901 gcgagacggc ggatgagatg gccgccgatg aagccggtgg atccggtgat gagaactcgc
      961 atctgctacc ttgagaagaa ggtgtcgggt gcgaggtgcc tggtgtcagg gcctcaccct
     1021 gaaccacctg acacccagca cccgatccct gatccctgcc gctatggaag cggaatcctg
     1081 tacgccagcc ccaccgcgac cgcgcgacga aaaagggaca tgagaccctc cttgggagtt
     1141 gcagccggaa tgtggcagga aggggttgaa acggcggtga atcagggagg aaagggcggt
     1201 caggcggtca ggaggacaaa ggcggacaag gggcgaacac gagcgggcac gagcgaaacc
     1261 gaccgcctga ccgcccgacc gcctgaccgc ccctagggga tctgccctgc gcgatgcagg
     1321 ctccagctca atccgacact ccccacgaac cgcgtctcgc tgctcccctt cacgtgcccc
     1381 tcattcacga tgcgatgcgc cccgccgtcg gccgtcagcg tcacgggccc ggcgctccag
     1441 tgcacgccga gggcgagccg cacggtgtgc tcgacggtgc cctggaacag cgacgcggtg
     1501 gcgggatagt cgggaggcgc cgggtgcgcc aggcggggat cgccctgacc ctggcgcagc
     1561 agcgtcacct cgggcgacag cagcacgccg cccgggccca cgacgctgag ccgtgcggtc
     1621 gcctgatcgt agtcggcgaa gttgcgtccg gtcccgaggc cgtggtacag cggcacctgc
     1681 aggttgtcct cgttgcggta ggtgagattc gccacctggg tatagaagag cgtccatgcc
     1741 gcacccgcca ccccgccctt ggcgcccacg gtcaatcccc agctggtggg cttctggtcg
     1801 gtgagcgtct tccggtccac ctggatgtcg tccagcatca gctgcgcgta ggccgtgacg
     1861 ccgccgcgcc gctcgagatc cagaccgacg aagctgttga cgttggtccc cgtgttcagc
     1921 tgctccagga acccgaggtt caggatgttg aggtaccagg gctcgaggtc gcggtcggcg
     1981 cccgagagca ccgacccttc ccacagcgcc gccgtccagc gcccgggcgg gcgcagccac
     2041 agccggtgct gcaccatgta gcgccggttg atccggccga gcgagtcggt gcgcgagtcg
     2101 agctgcgtga cgatcgcctg cagctggacc gccgcggtgc ccagcgtgat ccccaggtgg
     2161 tcgagcccgt atggatcgtc cgacagcaac agcccctgca ccacgctcgg cccccagttg
     2221 cggtccatgc gcccgaagaa gagctcgccg aagccccact gcgccgagag gtaggattcg
     2281 gccatccgcc cggcgatggc gcggtccttc cggccgaacc agtcgcggtc gtacttgagg
     2341 cgcgtatcga acagcgggtg cgtcaccgcg acgacgggcc cgagcgccag ggtgagggcc
     2401 aggccgcccg agaggaagcc gcgcgacacg gtatcgccgc gcggcggcgc gccgcggaac
     2461 agctccagcg gatcgcgcag gctgtgggtc gccgccgcca cgccgagatc cgcgtccacc
     2521 cgccccgacg gcccctgctc gtgtcgccgc aggtcatgga gcaacgatcg gaccactccc
     2581 cactccccgc tccccagtcg cgtgctgtcc atcgcctcga gcgcgcggac cagatcgtcg
     2641 gcgcggaccg gccgcgtgag cggcgaaggg tccaccatct ttcccgcggc gatgaagtgc
     2701 tcgacatagg gattccccca gtagttcagg gggacgtagg gagaggcggt ttggccgcag
     2761 agcggcgctg ccagggcgag gagcgcgatg aggattgcgg attgccgatt gcggattgcg
     2821 gattgaagac cattggggat tgcggattgt ggattgtgga ttggaaagag ctgttcaatc
     2881 cgcaatcccc aatccgcaat ccgcaatggt ctcacggcat caccctcact cccacctcca
     2941 gcagggtgag cgcgccgacg ctgccattgg tgtatgtgct ggtgacctcg tagagctgct
     3001 ggcgctcgag gcgcagcccg ccgatgacgc ggttcccgag cgggtagtcg atgccggcgc
     3061 cgacgagcca gaacaggctc gtgtggtaca cgatgctgga ctggtccgtg gacttgagac
     3121 ggtaggcgcc gccgcccagc gtcaccaccg ggacgacggt gctgttgcgc gtcagctcgt
     3181 actcgccccg cacgagaccc tggatcaggc ccacggcgag gctggcggac ccggtgtgcg
     3241 cgcgcaccac acccagcccg aggccgccca gcacgcgcaa ccccggcgcc acgtcccggc
     3301 ccaccatcag ctcggtgcgg aagccgggcc ggccgttcac ggcgggcgcg ccgtcgaagc
     3361 tgtagccgaa cgccgcgccg ccggcgcgcg caccgtacac ccagccgccc ggcgcctccg
     3421 gcagcgtcac ctgcagcggc acccgtgcgc tgagccgctg cccctcgccc gccgcactca
     3481 cctcgagcgt ggcgcgcccg ttgcgccgcg gctcgagcac cagcgtgccc ggcgccgtgg
     3541 cgacgccgcg caggttgtgc aggccggcgg cggtgccttt caccgtcgcg gtctggccca
     3601 ggaggatcgc ctcgttgccc caggcgtcgc gcgccaccat gcgcaccgtc gccgttaccg
     3661 atgtcgccag gacgagcgag tcgacggggc gcccgccctg ctccaccacc agcgtctgcg
     3721 ccggtcccgc cgtggcgaac aggttcgtgg tgcgcgacac gcggcccgcg gtcgccgtga
     3781 tgaccaccgg gccggcgcgg tcgcccaggg tcacggtggt gcgcgccacg ccggaggagt
     3841 cggtgaccgt ttcggtgtcg ctgagcgccg cgttgacggg cgtgaagcgg acggcgtagc
     3901 cggtcaccgg cgcgcccgca gtgtcgcgca cctcgagcag cagccgctgg cgcagtgtgg
     3961 tgtgcacgct gccgcgctgc cccgtccccg cgatgaactg ggtgcggtcg tccgagatcg
     4021 cgggggtttc gatgcgcgcg gtgatgctgg cgagctgcgt gccgcgcgcg aacacgccga
     4081 tcgtcccgcc cgagtgcagc gcgccggcgg ggatcggcag cagcgccgtc ccggtgccgt
     4141 ccgtattgac gggcggcagg gggccgagcc gggcgccgag gccgcgcagc tcgaccggca
     4201 gcgcggcgac gggcaccccg cccgacccgc gcaccaccac gcgcaccacc gccgggaagc
     4261 ggctgccttc ccgcaccacc acgaccggcg ggaggacgtc cgccagcaac gacggcgcca
     4321 cggtgacgag ctgcagcgtc gcctgggccg gcatgtcgcc caccgcgatg acctcgaggg
     4381 tgtaggtccc cgcctgcatc cccgcgagca cgcggaaggt cgccgtgccg cgcgcatcgg
     4441 tcaccgctcc cggttcctgc gccgccccgc cggggatggc ggtggcgcca cgcagctgca
     4501 gcgacttgcc gcccagcggc acgccgccgc gcaacagccg caccgtgagc gccacctccg
     4561 accccgccgt ggcccgcagc cgtgccggcg cgacgatgcg cggtacgccg ctgccgccgg
     4621 gacgcgtggc gcgcgacgcg cagccgtcga tggcgtcgag cacctccttg tcggcgcccg
     4681 cttcggccag atcgaagcgg tcccggggac tgggcgtgaa ggcgatgcag cggcggcgca
     4741 ccatcagggc gacgttgccg ggaggccggc cggacgtgag caggcgaatc aggtcggtct
     4801 tgcgcagcgg gccggactgg gagacctgcg cggccacggg cccggcggcg gcgagcagca
     4861 acagcaagac ggacgaccaa ttgcggattc tcatgaaagg ctcccgcctc acggtatcgc
     4921 ggctgcagcc gcgactcggc tttctatcgc tgacaacgac gcgcgtcacc tcgcgtgaca
     4981 gctcagccga gccgcgagct tactcgcgac tcgttcgctg gcacgagctt atgcacctcc
     5041 ggcgggccgc gcaatcggcc ggcggctgac gtgagcgccc tcaatccgtc tatccgccaa
     5101 tccgccttac ttcggcacga gcaccacgcc gcacacccgt tccgccacgc ctcgcgtcgg
     5161 acgcgagacc gcgaccgcgg cgccggcgac ggcgagcgca ccgagcgccc agtgcagcca
     5221 gcgggggagg cccggagacc gcggcgcggc gccgggcggc gccgcctgcg gcgcggacac
     5281 gcacatcgcc ccgctcccgc ccgcccgccc gcaccgagac gcgcacgccg cccggccacg
     5341 cggtgagcgc cagcagcagc agcagcggcg accaggtgag gggaagagag accttcaagt
     5401 accgtaga
//
LOCUS       DBNGZW010000315         4010 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_430003, whole genome shotgun sequence.
ACCESSION   DBNGZW010000315 DBNGZW010000000
VERSION     DBNGZW010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4010
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_430003"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2886
                     /locus_tag="AC16JC_07455"
     CDS             <1..2886
                     /locus_tag="AC16JC_07455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5164569.1"
                     /translation="AVNAIPGVVDLKSTSAFGVSVVNVYFRDDVDLYLARQLVSQRLL
                     DLQEDIPASVGRPTLAAATTGLGQVYQYVLRGEGRSPMELRTLEDWVVRPALRSVRGV
                     ADVQSWGGEVAQYQVTVDPQALRQYAVSLADVARALEENNENAGGGVIVRNDEEQLVR
                     GLGRLGGVDDIGDVMVTNREGVPIRVRDVASVTVGPEFRRGLVSRDGRGEVVAGIVLR
                     LVGENTARVIAAVERRVDQLNATLPPGVRIVPFYNRATLIASAIATVRDALLEGGVLI
                     VIVLLLFLGDVRSALIVTALLPLSALAAFLLMRRFGVTANLMSLGGLAIGLGMLVDGG
                     VVMVENVHRHLTEATGAAAPLEQVGTAAREVGRPIVFAIGIIVVVFLPLFTLQGVEGK
                     MFAPMAFTISFALLASLLLSLTVIPVLCALLLRRRQGARQSGATDRLRGTYRRLLERV
                     LRHRRAALAVAVSALAASLALVPFLGTEFIPSLNEGSLVVRVTMAPSISLDAAGAVAR
                     EVERNLRTFPEVTTAVSFVGRAEASGEPEPVSNAEIYVGLRPPSQWTTARDRDALITA
                     MRARLDRIPGTLVTFSQPIQIRQDELISGVKAPLAIRLFGDDLDTLAAKGAQIAGIVR
                     SIRGAVDVAAEAIGGQPQVRIRANRDALARYGLSTDAVQEVVRRAVGGEPSGQVFEGR
                     RRFDILLRFAPQARSDVAAISQTLLDAPGGQRVPLSAVADVSLTFGPPEIRHDEGQRR
                     LVVEADVEGRDMGSFVSEAGRRVRAAVTLPPGFRVEWGGQFELQERAMRRLAIVVPTA
                     LVLILSLLYGAFGSLGNALLVILNVPFGLIGGVASMFISRQYLSVPAAVGFIALFGVA
                     VLNGVVLVTYVDQLRGEGYSLEEAVRRGAELRLRPVLMTALVASLGLVPLLFAGGTGS
                     EVQRPLATVVVGGLLTSTVLTLFLLPALYPWFAAIRWRRRESRGTPV"
     gene            complement(2892..3857)
                     /locus_tag="AC16JC_07460"
     CDS             complement(2892..3857)
                     /locus_tag="AC16JC_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR5164570.1"
                     /translation="MKPTRELPNDAALPGLAAIRDRGPMATMPALGMDGPVEVWLVGY
                     SAGKRATLEARSGHRRVAIKVCATDPMPEVRLYAELGERGLARVGPAAAAGEVVRVPP
                     LLAWHPGLRLVATGWLEGCPLNQLVKEGRGERAGELAARWFRRVASLPSSLGSGYGPA
                     HFLDRAPGWVDTLVEADPVLGNMAVTVARALMCTPPPVGFPHLVHGTLYARHIIDMGD
                     GPGLIDWDAFGHGPLEFDAGTLLATIWRIQLSNPDAADAVARAERAFYAGTTGLLDAR
                     ALSWYRAAALLGAAHRLKARSKGNWKARALALLSEAGRSAVVAAA"
BASE COUNT          544 a         1380 c         1459 g          627 t
ORIGIN      
        1 gcggtcaacg ccattccggg cgtggtggac ctgaagtcca cctccgcctt cggggtctcg
       61 gtggtcaacg tgtacttcag ggatgacgtc gatctgtatc tcgcccgcca gctggtgagc
      121 cagcgactgc tcgatctgca ggaggacatt cccgcgagcg tgggccgacc cacgctggcg
      181 gcggccacga ccgggctggg acaggtctac cagtacgtgc tccggggcga gggccggagc
      241 ccgatggagc tgcgcacgct ggaggattgg gtcgtgcgcc ccgcgctacg cagcgtacgc
      301 ggcgtggccg acgtgcagtc ctggggcggc gaagtggcgc agtaccaggt gacggtggat
      361 ccccaggcgc tgcgtcaata cgccgtctcg ctggccgacg tggcccgggc gctggaggaa
      421 aacaacgaga acgcgggtgg gggggtcatc gtcagaaacg acgaggagca gctcgtccgg
      481 ggcctgggcc ggctgggggg ggtggacgac atcggggacg tgatggtgac gaatcgggag
      541 ggggtcccga tccgcgtccg cgacgtcgcc agcgtgacgg tgggacccga gttccgtcgg
      601 ggtctagtca gccgcgacgg gaggggcgag gtcgtcgcgg ggatcgtgct gcggctggtg
      661 ggggagaaca ccgcccgggt aatcgccgcg gtcgagcggc gggtggacca gctcaacgcc
      721 accctgccgc cgggggtgcg catcgtgccg ttctacaacc gtgccacgct gatcgcgagc
      781 gccatcgcga cggtgcgtga cgccctgctc gagggcgggg tgctgatcgt gatcgtcctg
      841 ctcctgttcc tgggcgacgt caggagcgca ctgatcgtga ccgcgctgct gcctctctcc
      901 gcgctcgcgg cgttcctgct catgcgccgg ttcggcgtca cggcgaacct catgtcgctc
      961 ggcggcctgg caatcggtct cgggatgctg gtcgacgggg gcgtggtgat ggtggagaac
     1021 gtacatcggc atctcacgga ggcgaccggc gcggccgcac cgctggagca ggtgggcacg
     1081 gccgcacggg aagtcgggcg gccgatcgtc ttcgccatcg gcatcatcgt cgtggtcttc
     1141 ctgccgctct tcaccctgca gggcgtcgaa ggaaagatgt tcgcgccgat ggcgttcacc
     1201 atcagcttcg cgctgctggc ctcgctgctg ctcagcctca ccgtcatccc ggtgctctgc
     1261 gccctcctgc tgcgacgacg gcagggcgcg cgccagtccg gggcaaccga ccggttgcgc
     1321 gggacctacc ggcggctgct cgagcgggtg ctgcgccatc gtcgcgcggc gctggcggtc
     1381 gcggtctcgg ccctcgccgc cagtctcgcc ctcgtgccct tcctcggcac ggagttcatc
     1441 ccctctctca acgagggctc gctcgtcgtc cgggtgacca tggcaccgtc catctccctc
     1501 gacgcggccg gcgccgtcgc ccgcgaagtc gagcgcaatc tgcggacgtt cccggaggtc
     1561 accacggccg tgtcgttcgt cggtcgggcc gaggcgagcg gcgagcccga gcccgtgagc
     1621 aacgcggaga tctacgtcgg gttgcggccg ccgtcgcaat ggacgacggc ccgggacagg
     1681 gatgcgctga tcacggcgat gcgggcgcgg ctggaccgca ttccgggcac gctcgtgacc
     1741 ttctcgcagc cgatccagat ccgccaggac gagctgatct cgggggtgaa ggcgcccctc
     1801 gcgatccggc tgttcgggga cgacctggac acgctggcgg ccaagggagc gcagatcgcc
     1861 gggatcgtgc ggagcatccg cggcgcggtc gacgtggctg ccgaggcgat cggcggtcag
     1921 ccccaggtcc ggatccgggc gaaccgggac gcgctggcgc gctacgggtt gagcaccgat
     1981 gccgtgcagg aagtggtgcg gcgcgcggtg ggtggcgagc cgagcggaca ggtgttcgag
     2041 gggcggcgac gcttcgacat cctgctgcgg ttcgctccgc aggcccgctc ggacgtggcg
     2101 gccatctcgc aaacgctgct cgacgctccc gggggacagc gggtgccgct gtcggcggtg
     2161 gccgatgtgt cgcttacttt cgggccgccc gagatccgac atgacgaggg ccaacggcgc
     2221 ctcgtggtgg aagcggacgt ggaggggcgc gacatgggga gcttcgtctc cgaagccggg
     2281 cggcgtgtgc gtgcggcggt caccctgccg ccggggtttc gcgtcgagtg gggcggccag
     2341 ttcgagctgc aggagcgggc gatgcgccga ctggcgatcg tcgttcccac cgcactggtg
     2401 ctgatcctgt ctctcctgta tggcgcattc gggtcgctcg ggaacgcgtt gctcgtgatc
     2461 ctgaacgtgc cgttcggcct gatcggcggc gtcgcgtcga tgttcatctc gcgccagtac
     2521 ctcagcgtgc cggcggcggt ggggttcatc gcgttgttcg gtgtcgctgt gctgaacggc
     2581 gtggtactgg tgacctacgt cgatcagttg cggggtgagg gatactccct cgaggaggcg
     2641 gttcggcggg gggccgagct gcggctgcga ccagtcctca tgacggcgct cgtcgccagc
     2701 ctggggctgg tgccgctgct gttcgccggc ggcaccggtt ccgaggtgca gcgaccgctc
     2761 gcgaccgtcg tggtgggcgg cctcctgacg tcgacggtgc tcacgctgtt cctgctgcct
     2821 gcgctctacc cgtggttcgc tgcgattcgg tggcgccgtc gggagagccg cggcacacca
     2881 gtctgaccca ttcaggctgc ggccaccacc gcggaacgcc cggcctcgga caacagcgcc
     2941 agcgcccgcg ccttccagtt gcctttggaa cgggccttca gccgatgcgc ggcaccgagc
     3001 agcgccgccg cccgatacca cgacagcgcg cgggcgtcca gcaggccggt cgtgcccgcg
     3061 taaaaggctc gctcggcccg ggcgaccgcg tctgccgcgt ccggattcga caactgaatg
     3121 cgccagatcg tcgcgagcaa cgtgccggcg tcgaattcca acggcccatg accgaaggca
     3181 tcccagtcga tcagaccggg gccgtctccc atgtcgatga tatgacgggc gtacagcgtc
     3241 ccgtgcacca ggtgcggaaa ccccaccggc ggcggcgtgc acatcagggc ccgcgccacc
     3301 gtcaccgcca tgttcccgag taccggatcg gcctcgacga gggtgtcgac ccagcccggc
     3361 gcccggtcca ggaagtgcgc cggcccatag ccggaaccga ggctcgacgg cagcgacgcg
     3421 acgcgccgaa accagcgcgc ggctagctca ccggctcgct ccccgcggcc ctccttcacc
     3481 agctgattca gcggacatcc ctccaaccac ccggtggcca ccaaccggag gccaggatgc
     3541 caggccagga gcgggggcac gcgcaccacc tcccctgcgg ccgcggccgg gccgaccctc
     3601 gccagcccgc gctcgcccaa ttccgcatac agccgtacct cgggcatcgg atcggtggcg
     3661 cagaccttga tcgccacccg ccggtgcccc gagcgcgctt cgagggtggc gcgcttgccc
     3721 gccgaatacc ccacgagcca cacctctacg gggccgtcca tgccgagggc aggcatcgtc
     3781 gccatcggcc cgcggtcacg gatcgccgcc agtccgggca gcgctgcgtc gttcggcagc
     3841 tcgcgtgtcg gcttcatccg gccgccgcag cgagacgtgc ggcctcggcg agcagcggtt
     3901 gcgccgcggc cacggcgtcg tcctccaaac gacgcagctt cttgtgcgcg agacgcaaca
     3961 acgtaacggc ctggtaccag gcgagcgccc gctcgttcag gagccccttc
//
LOCUS       DBNGZW010000316        10108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1042841, whole genome shotgun sequence.
ACCESSION   DBNGZW010000316 DBNGZW010000000
VERSION     DBNGZW010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 10108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10108
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1042841"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..472)
                     /locus_tag="AC16JC_07465"
     CDS             complement(125..472)
                     /locus_tag="AC16JC_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014910486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR5164571.1"
                     /translation="MTAAADCLFCKVARGEIPAGVVKRAGRLLAFRDINPQAPTHLLV
                     IPTEHVGSLDETGDGALLGELLLFARDVARDAGLAERGYRVVLNTNADGGQTVFHLHA
                     HLLGGRAMRWPPG"
     gene            complement(469..1656)
                     /gene="dnaJ"
                     /locus_tag="AC16JC_07470"
     CDS             complement(469..1656)
                     /gene="dnaJ"
                     /locus_tag="AC16JC_07470"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR5164572.1"
                     /translation="MSRRGAGDYYALLGVERDATEAEIKKAYRKLAMEFHPDRNPAPE
                     AEERFKQITEAYEVLRDPEQRAAYDRFGEAGLRGGAAGGPFGGFAHFDLSEALNIFMR
                     DFGGLGGFDVLFGGGERSRRDRRRGPDLKVSIRLTLSDVATGATKSLKLRTLDVCKTC
                     HGTGAKEGTKAATCGTCGGSGEVRRAAQSMFGQFVSVSPCPACAGEGTVIREPCPTCR
                     GDGRVKAEKTVQLDVPAGVADHHYLTVRGQGVPGPRNGPPGDLIAVLEILEDPRFERH
                     GDDLVFDLPVSFSQAALGSEAEIPTPYGTAPLKVQPGTQTGTIYRLRGKGLPRLGSGG
                     HGDLHVRIQVWTPTRLTPEQERAFHDLAQVEGEPPSSESLGRGFWNKLREAFGGSSGG
                     SGT"
     gene            complement(1653..2705)
                     /gene="hrcA"
                     /locus_tag="AC16JC_07475"
     CDS             complement(1653..2705)
                     /gene="hrcA"
                     /locus_tag="AC16JC_07475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HIR5164573.1"
                     /translation="MATPQLNDRERLVLGAVIETYVQTAEPAASRTIAKRFGLGLSAA
                     TIRNTMSDLEEKGFLYHPHTSAGRIPTDLAYRMYVDALMRLPAVTLDEAHQIREELAG
                     PRNAVDQILARAAQVLSVLTQELGMAAGPSLDEAVLERLELLAVSSERLLLVMTLKSG
                     AVRTIFVEVPSHMAAEAVVQVAVVLNERLAGLTLKEIRATLAARLRDATPSRESSELL
                     NIFVQEADELFDVPAAGTTGPFSGGVLLSSAQLLAGQPEFASTERLQGLLEVTERRDL
                     LREALAQRTSPGLTITIGSEHVDPKLAAFTLVTSSYRSGPLSGVIGVLGPTRMPYDKI
                     VALVDHTSRLVQELLQ"
     gene            complement(2736..3845)
                     /gene="hemW"
                     /locus_tag="AC16JC_07480"
     CDS             complement(2736..3845)
                     /gene="hemW"
                     /locus_tag="AC16JC_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR5164574.1"
                     /translation="MHHLYLHVPFCARRCSYCDFSIAVRRRIPAEEYIAAIRAEFERQ
                     EANGPLQTIYLGGGTPSLLPSDAVVTLLQSILGHTPPTDTIEVTLEANPEDITPVAAR
                     AWRSAGINRASLGAQSFDDRVLRWMHRSHDAARVSAAVETLRSAGIENISLDLIFALP
                     PELERDWRSDLDRALALEPAHLSLYGLTVEPRTPLARWVSRGATAAPDDERYAEEYLL
                     AHERLAEAGYAFYEVSNAARGGRRSRHNSAYWSGRPYLGLGPAAHSFDGRTRRWNIAA
                     WEAYRRAVADGGSPVESEETLTEEQRELERLYLNLRTADGLPSTVLHHPPQPVALLLA
                     RGWMVQDGGRLRCTPEGWLRLDSLVHALTGSAAIS"
     gene            complement(3894..5069)
                     /gene="dprA"
                     /locus_tag="AC16JC_07485"
     CDS             complement(3894..5069)
                     /gene="dprA"
                     /locus_tag="AC16JC_07485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR5164575.1"
                     /translation="MEAPLHLRARDDELAAYLSLTQVPGIGAARLRTLLAAFGSAGGA
                     IVAPAGALAALPGFGPAAAAAVRGARRRTGAEILEALQRIGATAILPGDPTYPPLLAE
                     IGEPPAVLYVWGDVALLARPAAALVGSRNHSAYGAEAARVLASGVARAGAVVVSGMAR
                     GLDAVAHEAALEVGGATVGVLGNGFGVVYPAANRVLYEKVVGQGCLVTELPPGERPRV
                     STFPRRNRIISGLAGVTVVIEAAPGSGALITADCALDQGRSVLAVPGPITSTTSVSCN
                     KLIQQGAQPALAPADVLEELGLAAPEGETPGRPADRPPVWPEDLSGIERSLCESLGAA
                     PAHVDTLVRATGLDSGDVLAALTELELRGLVAQKPGMIFELRIGDCGLRIGGGTNLH"
     gene            complement(5027..5998)
                     /gene="obgE"
                     /locus_tag="AC16JC_07490"
     CDS             complement(5027..5998)
                     /gene="obgE"
                     /locus_tag="AC16JC_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011362883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR5164576.1"
                     /translation="MTAGPGGAGACSFRREKFVPKGGPDGGDGGSGGSVLVRADANLS
                     TLLDYRYRTHWKAERGIHGKGKQMTGKTGADLVLLVPPGTEIHDADSGLLLGELLHAG
                     DTVLVAKGGRGGRGNARFATPTHQSPCEWEPGEFGEEKRLELVLKLIADVGLLGEPNA
                     GKSTLLSVVSAARPKIADYPFTTLEPYLGVVGLSDQRSFVMADIPGIIEEAHAGKGLG
                     TRFLQHVERTRLVAVLIPADSPDPQATYDLLRREAALYGADLAAKPHVVVITKADLLP
                     PGAAPPAPRTVDDAPVVVVSAVTGAGLPQLLETLWKRLCTSAPATTS"
     gene            complement(6016..6411)
                     /locus_tag="AC16JC_07495"
     CDS             complement(6016..6411)
                     /locus_tag="AC16JC_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004071806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5164577.1"
                     /translation="MTPEELRNRTRVFAGEIVRLVEGMPRSRSAEVIGNQLMRAGTSV
                     AANYRSACRARSLREFIAKMGIVEEEADETELWLDLAAEQRLGPPQRVEALRAEAGQL
                     VAIVVSSIRTARRSGRSNPQSAIRNPQCS"
     gene            complement(6457..7110)
                     /locus_tag="AC16JC_07500"
     CDS             complement(6457..7110)
                     /locus_tag="AC16JC_07500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR5164578.1"
                     /translation="MTQKSPPPASVAPTLDPRTIALVRVAMAVSRGDEHAIRERMRAA
                     RGAGVPVLWMEELLLQSFLNVGYPLALAAFAIWRELAGPEVDGGESLLHVEHGFWGER
                     GAQRCAEVYGRTYHKLRLNLRDLHPALESLVIVDAYGKILGRPGLDAARRELCTLAAI
                     TMLQAPRQLHAHLRGALNLGWSREDVDAVLVFAEQDLGAARALKVWELWADVRERKL"
     gene            complement(7115..8131)
                     /locus_tag="AC16JC_07505"
     CDS             complement(7115..8131)
                     /locus_tag="AC16JC_07505"
                     /inference="COORDINATES: protein motif:HMM:NF017864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HIR5164579.1"
                     /translation="MRVEERRGDLTETVHRVHVAVVDADGALVARAGDPGRVTFWRSA
                     AKPFQAMPLVADGAAERFDFSDAELALCCASHSSEPGQVEMVRALLEKIGCREDDLRC
                     GPHTPLAEHIAKDYQARGVRLTPVYSNCSGKHTGMLALARHHEWPTADYHRLAHPVQQ
                     RCREEVSRWTTLPADRIGTAVDGCGVVCYALPLKNMALAYANLGMGDGGRGTSTTGEK
                     GGASSPVPRPSSHVVAAMLRHPELIAGERRPCTEMMTAFPGRLITKVGAEGVYCALLV
                     REGLGVALKVEDGHGTSAVLAMARTLEELSLRQQPESVTRRPVLNTRGEVVGELRVFG
                     SLER"
     gene            8229..9248
                     /locus_tag="AC16JC_07510"
     CDS             8229..9248
                     /locus_tag="AC16JC_07510"
                     /inference="COORDINATES: protein motif:HMM:NF012515.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional heptose 7-phosphate kinase/heptose
                     1-phosphate adenyltransferase"
                     /protein_id="HIR5164580.1"
                     /translation="MELTRDRLEALLAGMGARRIVVLGDAMLDVYLAGEVDRISPEAP
                     VPVVTVRQRRVALGGAGNVAANVAAIGAEGRLVAPIGDDARGDSLRGELAQHRLGDAW
                     LVVVPERPTTSKTRVVARGQQVVRIDEEVDDPVPERSAQALITALERAMADADALLIE
                     DYNKGALTPAVIERAMAVARRRGVPVVVDPKFRHFFAYRGATVFKPNRRELEQAMGAA
                     LDLAHPDALPAVLDRLGVDNLLLTLGAEGMGLLTKDRQLTRIPALAREVYDVSGAGDT
                     VTAWVGTALAAGATMREAAEVATYAAGIEVGKAGVATVSPAEVVAAYDAAQDQVGRLR
                     RSGLL"
     gene            9385..9457
                     /locus_tag="AC16JC_07515"
     tRNA            9385..9457
                     /locus_tag="AC16JC_07515"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9418..9420,aa:Ile,seq:cat)
     gene            9567..9640
                     /locus_tag="AC16JC_07520"
     tRNA            9567..9640
                     /locus_tag="AC16JC_07520"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9601..9603,aa:Val,seq:cac)
     gene            9756..>10108
                     /locus_tag="AC16JC_07525"
     CDS             9756..>10108
                     /locus_tag="AC16JC_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164581.1"
                     /translation="MRRVLIAGHVYVEPTARGKLRALAGRGVELTVAVPQRWRDPILD
                     RTRETTWERQQALEVFPIVMRNVADPMQARFGARALAALVRDKRPELVQVEAEPETAL
                     ARQLVRLATRMRVPTV"
BASE COUNT         1577 a         3590 c         3366 g         1575 t
ORIGIN      
        1 cttcttcttg aagcgcttga gggcacgctc gaagctctcg tcgtcgtgga tgatgacttc
       61 gggcattgtc gttaactgtg gtagacgtgc caagttagcg gcccgggcgg agacaccgca
      121 aggcctaacc ggggggccac cgcatggccc gcccgcccag caggtgcgca tgcagatgaa
      181 agaccgtctg gccgccatcg gcgttggtat tcaacacgac ccgatagccc cgctcggcga
      241 gtcccgcgtc ccgcgcgaca tcccgggcga agagcagcag ctcgcccagc aatgccccgt
      301 ccccagtctc gtcgagtgag cccacgtgct cggtgggaat cacgagcaga tgcgtcggcg
      361 cctgcgggtt gatgtcacgg aacgccagca gtcgcccggc gcgcttcacc acgcccgcgg
      421 ggatctcacc ccgcgcgacc ttgcagaaga ggcaatccgc ggccgccgtc acgtgccgct
      481 ccctccgctg ctccccccga acgcctcgcg cagcttgttc cagaatccac ggccgaggct
      541 ctcggaggag ggcggctcgc cctctacctg ggcgaggtca tggaaggcgc gttcctgctc
      601 gggagtgagt cgcgtcggtg tccagacctg gatgcgtacg tgcagatcgc catggcctcc
      661 tgacccgagg cgcggcagac ccttgccgcg cagccggtag atcgtgccgg tctgcgttcc
      721 gggttgcact ttgagaggcg ccgtgccgta gggcgtcggg atttccgcct cggagccgag
      781 cgcggcctgg ctgaacgaga ccggcagatc gaacaccagg tcgtctccgt gccgctcgaa
      841 gcgcggatct tcgaggattt cgagcaccgc gatgaggtcg ccgggaggac cgttgcgggg
      901 tcctggcacg ccctgcccgc gaaccgtcag gtaatggtga tcggcgacgc cggccggtac
      961 gtcgagctgc accgtcttct ccgccttgac gcgtccgtcg ccgcggcagg tgggacacgg
     1021 ctcccgaatg acggtgccct cgccggcgca cgcgggacag ggcgacaccg acacgaactg
     1081 gccgaacatg gattgcgccg cccggcgcac ttccccgctg ccaccgcagg tcccgcacgt
     1141 cgccgccttg gttccctcct tggccccggt cccatgacac gtcttgcaca cgtccagcgt
     1201 gcgcaacttc agcgacttgg tggcgccggt ggcgacgtcg gagagggtga gccggatcga
     1261 gaccttgaga tcgggtccgc gtcggcggtc ccggcggctc cgctccccac cgccgaacag
     1321 gacatcgaat ccgcccagcc cgccgaagtc ccgcatgaag atattgagcg cttcggagag
     1381 atcgaagtgc gcgaagccgc cgaacgggcc gcccgcggct ccgccgcgca ggcccgcttc
     1441 gccgaagcgg tcgtaggcgg cgcgttgctc gggatcgcgc agcacttcgt aggcttcggt
     1501 gatctgcttg aaccgttcct ccgcctcggg cgccggattg cgatcgggat ggaactccat
     1561 cgccagcttg cgataggcct tcttgatctc ggcctcggtg gcgtcgcgct cgaccccgag
     1621 cagcgcgtaa tagtcgcccg ctccccgacg gctcattgca gcagctcctg cacaaggcga
     1681 ctcgtgtggt ctaccagcgc cacgattttg tcatagggca tgcgggtcgg gccgagcacg
     1741 ccgatcacgc cggacagcgg ccccgaacgg tacgacgatg tcaccagcgt gaacgccgcc
     1801 agcttcgggt cgacgtgctc gctgccgatc gtgatcgtca gccccggtga cgtgcgctgg
     1861 gcgagggcct cgcgcaacag atcgcgccgc tcggtcacct cgagcaagcc ctgcagtcgt
     1921 tcggtggagg cgaactcggg ctggccggcg aggagctgcg ccgacgacaa cagcacgccg
     1981 ccggagaagg ggcccgtggt cccggccgcc gggacgtcga acagctcatc ggcttcctgc
     2041 acgaagatgt tcagcagctc gctcgattcc cgactcgggg tcgcgtcgcg cagccgggcc
     2101 gccagcgtcg cgcggatctc cttcagcgtg aggccggcga ggcgctcgtt cagcaccacg
     2161 gcgacctgca ccaccgcctc cgccgccatg tgcgacggca cctctacgaa gatggtgcgc
     2221 accgcgccgc tcttgagcgt catcacgagc agcaggcgct cgctcgagac cgcgagcagc
     2281 tccagtcgct cgagcaccgc ctcgtcgagc gaagggccgg ccgccatgcc gagctcctgg
     2341 gtgagcacgc tgagcacctg cgcggcccgc gccaggatct gatccaccgc gttgcgcggt
     2401 ccggccagct cctcgcgaat ctggtgcgcc tcgtccagcg tgacggccgg cagacgcatc
     2461 agggcatcca cgtacatgcg gtaggcgaga tcggtcggaa tccggccggc ggaggtgtgg
     2521 gggtgataca gaaacccctt ctcctccaga tcgctcatcg tattgcggat ggtggcagcc
     2581 gagaggccga gcccgaaccg cttggcaatg gtccggctgg ccgccggttc ggcggtctgc
     2641 acgtacgtct caatcacggc gcccaggacg agacgctcgc ggtcgttcag ctgcggtgtg
     2701 gccaaagtgc ttccaacccc ttttgggcaa acaaattacg aaattgcggc cgacccggtc
     2761 aaggcgtgca ccagagaatc gaggcgaagc cagccttcgg gtgtgcaacg caggcgaccg
     2821 ccgtcctgca ccatccagcc cctggcgagc agcaatgcga caggttgtgg aggatggtgg
     2881 aggacggtgg agggcaagcc atccgctgtc ctcaggttca ggtacagccg ttcgagttcc
     2941 cgctgttctt cggtgagcgt ctcttccgac tcgaccggtg aacctccgtc cgccactgca
     3001 cgccggtagg cctcccacgc cgcgatgttc cagcgacgcg tgcggccgtc gaaggaatgc
     3061 gcggcgggtc ccaggccgag atagggcctg ccgctccagt acgcactgtt gtgccgcgag
     3121 cggcgacctc cgcgcgcggc gttggagact tcgtagaacg catacccggc ttcggccagc
     3181 cgctcgtgcg ccagcagata ctcctcggca tagcgctcgt cgtcgggcgc agcggtggcg
     3241 ccgcgcgaca cccagcgagc cagcggggtg cgcggttcga cggtcagacc gtagagggaa
     3301 aggtgagcgg gctccagcgc cagcgcccgg tcgagatcgc tgcgccagtc gcgctccaac
     3361 tcgggcggca gggcgaagat cagatcgagt gagatgttct cgatgccggc gctgcgcaac
     3421 gtctccacgg ccgcgctcac acgggcggcg tcgtgcgagc ggtgcatcca tcgcagcacg
     3481 cgatcgtcga acgattgagc gccaagggac gcgcggttga ttccggctga ccgccacgcc
     3541 cgggcggcga ccggtgtgat gtcttcgggg ttggcctcca gcgtcacctc gatggtgtcc
     3601 gtcggaggcg tgtggccgag gatggattgc agcagcgtga cgaccgcatc cgaagggagc
     3661 agcgacggcg tgccgccacc caggtatatc gtctgcagcg gcccgttcgc ctcttgccgc
     3721 tcgaattccg cccggatcgc cgcgatgtat tcctcggccg gaatccgccg ccgcaccgcg
     3781 atcgaaaagt cgcagtacga acagcgacgg gcacagaagg gcacgtggag gtagaggtgg
     3841 tgcacggggg aaacatagcc gattgcggat tgtggattgg ggattgcgga ttgtcagtgc
     3901 aggttcgtgc cgcccccaat ccgcaatcca caatcgccaa ttcgcaattc gaagatcatc
     3961 cccggcttct gcgccacgag accgcgcagc tcgagctcgg tgagcgcggc cagcacatcg
     4021 ccggaatcga gtccggtggc gcgcaccagg gtatccacgt gggcgggtgc ggcgccgagg
     4081 gattcacaca acgatcgctc gatgccgctc aggtcctcag gccacaccgg tgggcgatcg
     4141 gccgggcgcc ccggcgtctc gccttccggc gcggcgaggc ccagctcttc gagcacgtcc
     4201 gcgggcgcga gtgccggctg cgctccctgc tggatgagct tgttgcagct gaccgaggtc
     4261 gtgctcgtga tgggaccggg aaccgcgagc acactgcgtc cctgatcgag tgcgcaatcc
     4321 gccgtgatca gtgctccaga tcccggcgcc gcctcgatga ctaccgtcac acccgccaac
     4381 cccgaaatga tgcggttgcg gcgcgggaac gtgctgacgc gcggccgctc gcccggcggc
     4441 agctccgtca ccaggcaccc ctgccccacg accttctcgt acaggacgcg gttggccgcg
     4501 ggatacacca cgccgaaccc gttccccagc actcccaccg tcgcgccccc cacctcgagc
     4561 gccgcctcat gggcgaccgc atcgaggccc cgcgccatgc cgctcaccac caccgcgccg
     4621 gcgcgtgcca ctccggacgc cagcacacgc gccgcctcgg caccgtaggc agagtgattg
     4681 cgactgccga cgagggccgc ggcaggacgt gcgagtaacg cgacgtcacc ccacacatac
     4741 agcacggccg gcggctcgcc gatctccgcc agcagcggag ggtaggtcgg gtcacccggc
     4801 aggatggccg tcgcgccgat gcgctgcagc gcttcgagaa tctccgcgcc ggtccggcga
     4861 cgcgctccgc gcaccgccgc ggccgccgcc ggcccgaacc cggggagcgc cgccagtgca
     4921 ccggccggcg ccacgatcgc accgccggcg ctgccgaacg ccgccaacag cgtccgcaac
     4981 cgcgcggcac cgatccccgg cacctgggtc agcgacaggt acgccgctag ctcgtcgtcg
     5041 cgggcgcgga ggtgcagagg cgcttccaca gcgtctcgag aagttgggga agaccggcgc
     5101 cggtcacggc cgacacgacc accaccggcg cgtcgtccac cgtccgcggg gcggggggcg
     5161 cggcgcccgg cggcagcaga tccgccttcg tgatcacgac cacatggggt ttcgcggcga
     5221 ggtcggcgcc gtacagcgcc gcttcgcggc gcagcagatc gtaggtggcc tgggggtcgg
     5281 gactgtcggc ggggatcagc acggcgacga gacgcgtgcg ttccacgtgc tgcaggaatc
     5341 tcgtgccgag gcccttgccg gcgtgcgcct cttcgatgat ccccgggatg tccgccatga
     5401 cgaagctgcg ctgatcggac aggccgacca cgcccagata cggctcgagc gtcgtgaacg
     5461 gatagtcggc gatcttgggc cgcgccgccg acacgaccga cagcagcgtg ctcttgccgg
     5521 cgttcggctc gcccagcaat cccacgtcgg cgatgagctt caggaccagc tcgaggcgtt
     5581 tctcttcgcc gaactccccc ggctcccatt cgcacggcga ctggtgcgtc ggcgttgcga
     5641 agcgcgcgtt gccgcgtccc ccgcgtccgc cctttgccac gagcacggta tcgccggcgt
     5701 gcagcagctc acccagcagg agaccggagt cggcgtcgtg gatctccgtg ccgggcggca
     5761 ccaggagcac caggtcggcg cccgtcttgc cggtcatctg tttgcccttg ccgtggatgc
     5821 cgcgctcggc cttccagtgc gtgcggtagc ggtagtcgag cagcgtggac aggttcgcgt
     5881 cggcgcgcac caggacgctc ccgcccgacc caccgtcgcc gccgtcggga ccgcccttgg
     5941 gaacgaactt ctcgcggcgg aaggagcagg cgcccgcgcc gccggggccc gcggtcacgt
     6001 ggacgatcgc gcgatctatg aacattgcgg attgcggatt gcggattgcg gattggaccg
     6061 gccagatctg cgcgcagtcc gaatcgagga caccacgatg gcaactagct gccccgcctc
     6121 cgcccgcaat gcctcaacgc gttggggagg gccgagtcgc tgctcagcgg cgaggtcgag
     6181 ccacagctcc gtctcatcgg cttcttcttc gacgatgccc atcttggcga tgaactccct
     6241 cagagaacgc gctcggcatg ccgaccgata attcgccgcc acggacgtcc ctgcgcgcat
     6301 caactggttg ccaatcacct ccgcggatcg ggaacgcggc atgccttcca ccaggcgaac
     6361 gatttcgccg gcgaataccc tcgttcggtt tctgagctcc tctggcgtca cgacccctcc
     6421 ctgccaatcc gcaatccgca atctccaatc cgcaattcag agttttctct cccgcacgtc
     6481 cgcccacagc tcccagactt tcaacgcccg tgctgcaccc aggtcctgct cggcgaagac
     6541 cagcaccgca tctacatcct ccctcgacca gccgaggttc aaggccccgc gcaggtgcgc
     6601 gtgtagctgc cgcggcgcct gcagcatcgt gatggctgcc agggtgcaga gctctcgtcg
     6661 cgccgcatcg agccccggcc gacccagaat cttgccgtag gcgtccacga tcaccagcga
     6721 ctcgagcgcc gggtgcaggt cgcgcaggtt cagccgcagc ttgtgatagg tgcgtccgta
     6781 cacctcggcg caacgctgcg ctcctcgctc gccccagaat ccgtgctcca cgtgcagcag
     6841 cgactcgcct ccgtcgacct ccggccctgc cagctcgcgc cagatcgcga aggccgcgag
     6901 ggcgagcgga tacccgacgt tgaggaagct ctggagcagc agctcctcca tccacagcac
     6961 gggcacgccc gcgccgcggg ccgcccgcat gcgctcccgg atcgcgtgct cgtcgccacg
     7021 gctcaccgcc atggccacgc gcacgagcgc gatcgttcgc gggtcgagcg tcggggcaac
     7081 actagcgggc ggtggcgatt tctgtgtcat cgttctagcg ctcgagagag ccgaaaacgc
     7141 gcagctctcc gaccacctca ccccgcgtgt tgagcaccgg acgacgcgtg acggactcgg
     7201 gctgctggcg caggctcagc tcctcgagcg tgcgcgccat cgccagcacc gcggaagtcc
     7261 cgtggccatc ctccaccttg agggccacgc cgagcccttc gcgcaccagc aacgcgcagt
     7321 acacgccttc cgcccccacc ttcgtgatca gtcgtccggg aaacgccgtc atcatttcgg
     7381 tgcagggccg gcgctcaccg gcgatcagct cggggtggcg gagcatcgcc gcaacaacat
     7441 gggacgaggg acgagggacg ggggaggagg ccccaccctt ctctccggta gtactcgtcc
     7501 cccgtccccc gtcccccatc cctagattcg cgtaggccag cgccatgttc ttcaatggaa
     7561 gcgcgtagca tacgacaccg cacccgtcca ccgccgtccc gatccggtcg gcgggcaggg
     7621 tcgtccagcg gctcacctct tcgcggcagc gctgctgcac cgggtgcgcc aggcggtgat
     7681 agtccgccgt cggccactcg tgatggcgcg ccagcgccag catcccggtg tgcttccccg
     7741 agcagttcga gtacaccggc gtgaggcgca ccccacgcgc ctggtaatcc ttggcgatgt
     7801 gctcggccag cggcgtgtgc ggcccgcagc gtaggtcgtc ctcgcggcag ccgatctttt
     7861 cgagcagcgc ccggaccatc tccacctgcc ccggctcgct cgaatgggag gcacagcaca
     7921 gggccagctc ggcgtcgctg aaatcgaacc gctcggcagc gccgtcggcg accagcggca
     7981 tggcctggaa gggcttggcg gcggagcgcc agaaggtcac gcgcccgggg tcgcccgcgc
     8041 gcgccaccag cgcgccgtcg gcatcgacga cggcgacgtg aacacggtgg acggtttccg
     8101 tcagatcgcc gcggcgctcc tcgacgcgca tgcccggtcc cgggatggag acagcgccaa
     8161 tgataacagg tagattgccc gctcatgccg ccactcgtcc ctcgtcctcc gtcccccgtc
     8221 ccttcgtcgt ggaactgact cgcgatcgtc tggaggcgct gctcgccgga atgggcgcgc
     8281 gccgcatcgt cgtgctgggc gacgcgatgc tggacgtgta cctggccggc gaggtggacc
     8341 ggatttcgcc cgaggcgccg gtcccggtcg tcacggtgcg ccagcgccgg gtggcgctcg
     8401 gcggtgcggg caacgtcgcg gccaacgtgg cggcgatcgg cgccgagggc cggctggtag
     8461 cgccaatcgg tgatgacgcg cgcggcgact cgttacgcgg cgagcttgcc caacaccggc
     8521 tcggcgacgc ctggctcgtg gtggtgcccg aacggcctac gacgtccaag acccgtgtgg
     8581 tggcccgtgg ccagcaggtg gtccgcatcg acgaggaagt ggacgatccc gttccggagc
     8641 gctcggcaca ggcgctgatc acggcgctgg agcgcgccat ggccgatgcc gacgcactgc
     8701 tgatcgagga ctacaacaag ggcgccctca cgcccgcggt gatcgagcgg gcgatggccg
     8761 tcgcgcggcg gcgtggcgta cccgtggtgg tcgatcccaa gttccgccac ttcttcgcct
     8821 atcgcggggc caccgtgttc aagcccaacc gccgggagct cgagcaggcg atgggggccg
     8881 cgctcgacct ggcccacccc gatgccctcc ccgccgtgct cgaccggctc ggcgtggata
     8941 acctgcttct caccctcggc gccgagggga tgggattgct gaccaaggac cggcaactga
     9001 cccgcattcc ggcgctggcg cgcgaggtgt acgacgtttc gggcgcgggc gataccgtca
     9061 cggcctgggt gggcaccgca ctggccgcgg gagcgacgat gcgcgaggcg gccgaagtgg
     9121 ccacctatgc cgccggaatc gaggtcggca aggccggcgt ggccaccgtc agtccggcgg
     9181 aggtcgtggc ggcctacgat gcggcgcagg atcaggtggg gcggctgcgg agaagcggac
     9241 tcctgtgatt gcggattgac gattgcggat tgcggattgg gaatgtgcgt tctcggaccc
     9301 gtgggccccc atgggaacaa tccgcaatcc ccaatccgca atccacaatg ctcccctcga
     9361 gcgttatatt ttccccctcg tatcgggccc ttagctcagc ggttagagca gcggactcat
     9421 aatccgtcgg tcccaggttc aaatcctgga gggcccacgc taactgctag gcgttcaggc
     9481 gtggaggcgt acaggcgcct agtagtgcac cgccaagggt acttctgtac gcctgaacgc
     9541 cttgacgcct gtacgtcgag tagctcgggt ggttagctca gctggttaga gcgccggtct
     9601 cacataccgg aggtcacagg ttcgagtcct gtaccgccca tttccattgc ggattggcga
     9661 ttgtggattg cggattggat tcgcctcccc catttgtacg acacaaccca tccatgccaa
     9721 tccgcaatcc gcaatccaca atccgcaact agtgcatgcg ccgcgtcctc atcgccggac
     9781 acgtctacgt cgagcccacc gcccggggga agctgcgcgc gctcgccggc cgcggagtgg
     9841 agctcaccgt ggcggtaccg cagcgctggc gcgatcccat tctcgaccgc acgcgcgaga
     9901 ccacctggga gcgtcaacag gcgctcgagg tcttccccat cgtcatgcgc aacgtcgccg
     9961 accccatgca ggcgcggttc ggcgcccgcg cgctcgcggc cctggtgcgc gataaacggc
    10021 cggagctggt gcaggtcgag gccgagccgg aaaccgccct cgcgcgtcag ctcgtccgcc
    10081 tcgccacacg gatgcgcgtc ccgacggt
//
LOCUS       DBNGZW010000317         4630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1871821, whole genome shotgun sequence.
ACCESSION   DBNGZW010000317 DBNGZW010000000
VERSION     DBNGZW010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1871821"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2197
                     /gene="purL"
                     /locus_tag="AC16JC_07530"
     CDS             <1..2197
                     /gene="purL"
                     /locus_tag="AC16JC_07530"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIR5164582.1"
                     /translation="TGEEYARVEKLLGRTPTFAELGIFSALWSEHCSYKHSRPVLKTL
                     PTTGPQVVQGPGENAGVLRLPDGWAVAFKIESHNHPSAVEPYQGAATGVGGILRDVFT
                     MGARPVALLNSLRFGPLDEARNRYLCAGVVRGIGDYGNCVGVPTLGGEVDFAPGYSGN
                     PLVNAMCVGLLREQDLITAAAHGVGNVLLAVGASTGRDGIHGASFASEELTRESEQRR
                     PQVQVGDPFTEKLLLEASLELIASGHIVAIQDMGAAGLTSSSAEMAARGGVGVEIDLA
                     RVAAREPGMTPYEILLSESQERMLVVAKHDRVAQVQAITAKWELSATPIGRVTDDGMY
                     RCTWGDQVVVEIPGKPLVEDCPVYHPEAREDEATVHLRAERPAPHSPLPDITESLLTL
                     LDHPPLASKRWMFEQYDSTVRADTVIAPGGDAGVVRTRHADFGLAFTTACNARYVRLR
                     PYEGGKAAVAEAARNVACTGARPYGVTDCLNFGNPERPAVFFQFTEACRGIAEACRVL
                     ETPVTGGNVSFYNESPGGAVDPTPVIGMVGLLTRVEQAVRSHARVAGDRVFILGLTRD
                     ELGGSAYWEAVLGCRGGEPPQVDLAAERRLVSLLVAAAERGLVRSAHDCSDGGLAVAL
                     AEVAMGGPYHDGGFGLDLNLTAYGVPLAADALLFSETQARAVVTCAPERAAAIAALAA
                     EHGVPAFDSGAVGSAGGELQVRLRDGGIRMPVARLREVYFSAIPRRMGD"
     gene            2207..3604
                     /gene="purF"
                     /locus_tag="AC16JC_07535"
     CDS             2207..3604
                     /gene="purF"
                     /locus_tag="AC16JC_07535"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010942281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HIR5164583.1"
                     /translation="MCGIIGVSGVPDAARVAYLGLYSLQHRGQESAGLVAVDGEGVAR
                     SHRGMGLVADIFTEPVLAGLPGDVAIGHTRYSTSGSSILANAQPILARYREGPLALGH
                     NGNLTNAAQLRADLVDKGSIFQTSSDSEVLVHLIARSEAREIEDQLLDALERVEGAYS
                     LVVTIGRTLYAIVDPRGFRPLWLGKLGNGHVVASETCAFDLIGAAPLRELEPGEFLRI
                     SGPEVDRLPPLAVKPARRCIFELVYFSRPDSTIFGRSVDRVRRALGRELAREHPAPGA
                     DCVFSVPDSSNAMALGFADASGIKLEHGLIRNHYVGRTFINPTQAGRNAKVKIKFNPV
                     RDVLAGRRVVVVDDSLVRGTTSKGLVQMIRGAGAAEVHFRVASAPITGPCYYGIDTPT
                     KSELIASSHTIEEIRRHLGVDTLGYLSLDGMLRAAGGDSSGFCHACFSGQYPTPIPEQ
                     AAPDRQATPLVPVIV"
     gene            3627..>4630
                     /locus_tag="AC16JC_07540"
     CDS             3627..>4630
                     /locus_tag="AC16JC_07540"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR5164584.1"
                     /translation="MPSLERFVYSFGPGRADGSAAMRDVLGGKGAGLAEMANIGLPVP
                     PGFTISAKLCLRYLEDGTFPDDLREEVDRHLKQVEKVTERKLGDPVRPLLVSVRSGAA
                     VSMPGMMETILNLGLNDQTVEGLIAGSGNPRFAYDSYRRFVMMYGDVVFDLGKEPFDE
                     VLQAAKRRRKAARDIDLPPEDLKALVHEYKAIVRARCGVAFPDNPHTQLWGAIEAVFK
                     SWRTRRAIDYRRIHGIPDDLGTAVNIVTMVYGNLGEDCGTGVAFTRDCRTGERVLNGD
                     FLINAQGEDVVSGARTPDSIAKMKRGKFAKVYRELETIAEKLERHFKDVQDIEFTFEH
                     G"
BASE COUNT          693 a         1637 c         1579 g          721 t
ORIGIN      
        1 gacgggcgag gagtacgccc gcgtcgagaa gctgctcggc cgcactccga cgttcgccga
       61 gctggggatc ttctcggcgc tgtggagcga gcattgctcc tacaaacact cccggccggt
      121 gctcaagacc cttcccacca ccggtcctca ggtcgtccag gggcccggcg agaacgccgg
      181 ggtgctgcgc ctccccgacg gctgggcggt ggcgttcaag atcgagtccc ataatcatcc
      241 ctcggccgtc gagccgtatc agggggccgc aaccggcgtc ggcggcattc tgcgcgacgt
      301 gttcaccatg ggggcgaggc cggtcgcgct gctcaacagc ctgcgcttcg gtccgctgga
      361 cgaggcgcgc aaccggtatc tctgcgccgg tgtggtccgg ggcatcggcg actacggcaa
      421 ctgcgtcggc gttccgaccc tcgggggcga ggtggacttc gcccccggat actccggcaa
      481 cccgctggtg aacgcgatgt gcgtcggcct gctccgcgag caggacctga tcacggccgc
      541 ggcgcacggc gtgggcaacg tgctgctcgc cgtgggggcg agcaccgggc gggacgggat
      601 ccacggggcg tcgttcgcgt cagaagagct cacgcgggag agcgagcagc gccggccgca
      661 ggtccaggtg ggcgatccgt tcaccgagaa gctgttgctc gaggcgagcc tcgagctcat
      721 cgcctcggga cacatcgtcg cgatccagga catgggcgcc gcggggctca cgtcctcctc
      781 ggccgagatg gcggcccgcg gcggcgtcgg cgtcgagatc gacctcgctc gcgttgccgc
      841 ccgcgagccg gggatgacgc cctacgagat cctgctctcg gagtcgcagg aacgcatgct
      901 ggtcgtcgcg aagcacgacc gcgtggcgca ggtccaggcg atcacggcga agtgggagct
      961 ctccgcgacg cccatcggtc gcgtcaccga cgacgggatg taccgctgca cctggggcga
     1021 ccaggtggta gtcgagatcc cagggaagcc gctggtggag gattgcccgg tctatcaccc
     1081 ggaggcccgc gaagacgaag cgacggttca cctgcgcgcc gaacgtcccg ctccccactc
     1141 cccactcccc gatatcaccg agtcgctgct cacgctcctc gaccatccgc ccctcgcctc
     1201 gaagcgctgg atgttcgagc agtacgactc gaccgtccgg gcggacacgg tgatcgcgcc
     1261 aggcggcgac gcgggcgtcg tccgaacgcg tcacgccgac ttcgggctcg ctttcaccac
     1321 ggcctgcaac gcccgctatg tgcgcctgcg tccgtacgaa ggtggcaagg cggcggtcgc
     1381 cgaggccgcc cgcaacgtcg cgtgcaccgg tgcgcgcccg tacggcgtga ccgattgcct
     1441 gaattttggc aaccccgagc gaccggccgt cttcttccaa ttcacggagg catgccgcgg
     1501 catcgcggaa gcctgccgtg tgctggagac accggtcacg ggcggcaacg tttccttcta
     1561 taacgaaagc cccggcggcg ccgtggaccc gaccccggtg atcggcatgg tcggcctcct
     1621 gacgcgtgtc gaacaggcgg tgcggagtca cgcgcgggtg gccggcgacc gggtattcat
     1681 cctcggcctc acgcgcgacg agctcggtgg atccgcctat tgggaggcgg tgctcggctg
     1741 ccgcggcgga gaaccgccgc aagtggatct ggccgccgag cgccggctcg tctcgctgct
     1801 cgtggccgcg gccgagcgcg ggcttgtccg ctcggcgcac gattgctcgg acggcggcct
     1861 cgcggtggcg ctggccgaag tcgcgatggg cgggccgtat catgacggcg ggttcgggct
     1921 ggacctgaat ctcaccgcct acggcgtccc cctcgcagcc gacgcgctgc tgttcagcga
     1981 gacgcaggcg cgcgccgtcg tgacgtgcgc cccggagcgt gccgccgcca tcgcggccct
     2041 cgcggccgaa cacggtgttc cggcgttcga cagcggcgcg gtggggagcg ccggcggcga
     2101 gctgcaggtg cgcctgcgcg acggtggtat ccggatgccg gtcgcccggc ttcgggaggt
     2161 atatttctcc gcgatccccc gtcgcatggg ggactgagac gacactatgt gcggcatcat
     2221 cggcgtttcc ggcgttcccg acgcggcccg cgtggcctat ctcggactct acagcctgca
     2281 gcaccgcggc caggagtccg cggggctcgt ggccgtggac ggggagggtg tggcgcgctc
     2341 gcatcgcggc atggggctcg tggccgacat cttcaccgag ccggtactcg ccgggctgcc
     2401 gggcgacgtc gccatcggcc acacccgcta ctcgacgtcc ggcagctcga tcctcgccaa
     2461 tgcgcagccg atcctggcgc gttaccgcga ggggccactc gccctgggcc acaacggcaa
     2521 tctcaccaac gccgcccagc tccgggccga cctcgtagac aagggctcga tcttccagac
     2581 ctcctcggac agcgaagtgc tggtgcatct catcgcccgc tccgaggccc gcgagatcga
     2641 agaccagctg ctggacgcgc tggagcgcgt ggagggagcc tactcgctgg tcgtgaccat
     2701 cggacgcacg ctgtacgcca tcgtggatcc gcgcggcttc cggccgctgt ggctcgggaa
     2761 acttggcaac ggacacgtcg tcgcctcgga gacgtgcgcc ttcgatctca tcggcgccgc
     2821 tccgctgcgc gagctcgagc cgggtgagtt cctccgcatc tccggccccg aggtcgatcg
     2881 cctgccgccg ctggccgtca agccggcgcg gcgctgcatt ttcgagctgg tctacttctc
     2941 gcgtcccgac agcacgatct tcggccgctc ggtggaccgc gtgcggcgcg cgctggggcg
     3001 cgagctggca cgcgagcatc ccgccccggg cgccgactgc gtcttttccg tccccgactc
     3061 ctcgaacgcc atggccctcg gcttcgccga tgcatccggg atcaagctcg agcacgggct
     3121 catccgcaac cactacgtcg gccgcacgtt catcaatccg acgcaggcgg gccgcaacgc
     3181 caaggtgaag atcaaattca atccggtgcg cgacgtgctc gcgggcaggc gcgtcgtggt
     3241 ggtggacgat tccctggtgc gcggcaccac gagcaaagga ctggtacaga tgatccgcgg
     3301 agccggggcc gccgaagtgc acttccgggt cgcttcggcg cccatcacgg ggccgtgcta
     3361 ctacgggatc gacacgccga cgaagtccga gctcatcgcg tccagtcata ccatcgaaga
     3421 gatccggcgc cacctcggcg tggacacgct ggggtacctg tccctcgacg gcatgttgcg
     3481 ggcggcaggc ggtgactcct ccggattctg ccacgcctgt ttctccggcc aatacccgac
     3541 gccgatcccc gagcaggcgg ccccggaccg ccaggcgacg ccgctcgttc ccgtcatcgt
     3601 ctaatcaccc tcaccccaga ctcgccatgc cgtcgctcga gcgcttcgtc tactccttcg
     3661 gcccgggccg cgccgacggc tcggccgcca tgcgcgacgt cctgggcggc aagggcgccg
     3721 gtctggccga gatggccaac atcgggctgc ccgttccacc cggattcacg atctccgcca
     3781 agctctgcct gcgctatctc gaggatggga cgttccccga cgacctgcgc gaagaggtgg
     3841 accgtcacct caagcaggtg gaaaaggtca cggaacggaa gctgggcgat ccggtgcgcc
     3901 cgttgctcgt ctcggtccgt tccggcgccg ccgtgtcgat gccggggatg atggagacca
     3961 tcctgaacct ggggctgaac gaccagacgg tcgaggggct gatcgccggg tcgggcaatc
     4021 cccgcttcgc gtacgattcc taccgccgct tcgtgatgat gtacggcgac gtcgtgttcg
     4081 acctcggcaa ggagccgttc gacgaagtcc tccaggctgc gaaacgccgg cgcaaggccg
     4141 cccgcgacat cgacctgccc cccgaggacc tcaaggccct ggtgcacgag tacaaggcga
     4201 tcgtccgggc ccgctgcggg gtggccttcc ccgacaatcc ccacacccag ctctggggcg
     4261 ccatcgaggc cgtgttcaag agctggcgca cgcggcgcgc gatcgactac cggcgcatcc
     4321 acggcatccc cgacgacctg ggcaccgcgg tgaacatcgt gaccatggtc tacggcaacc
     4381 tgggcgagga ttgcggcacc ggcgtcgcat tcacccgcga ctgccgtacc ggggagcgcg
     4441 tgctcaacgg agacttcctc atcaacgcgc agggggaaga cgtcgtgtcc ggggcgcgca
     4501 cccccgattc gatcgccaag atgaaacgcg gcaagttcgc caaggtgtat cgcgagctcg
     4561 agacgatcgc ggagaagctc gagcgccact tcaaggacgt gcaggacatc gagttcacct
     4621 tcgagcacgg
//
LOCUS       DBNGZW010000318         5702 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_574600, whole genome shotgun sequence.
ACCESSION   DBNGZW010000318 DBNGZW010000000
VERSION     DBNGZW010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5702)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5702)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5702
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_574600"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..710)
                     /locus_tag="AC16JC_07545"
     CDS             complement(<1..710)
                     /locus_tag="AC16JC_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR5164585.1"
                     /translation="MTATEQQAIGQRLREHATLGGAPLQELEWLAANGDMRRYAAGEM
                     PIRQGQLAGMNGLMVVLTGHVVLYVNRGRGRRKLAEWHGGEVSGVLPYSRVTVAIGDG
                     VAESDSEIWLVDRDRFPEMIRECPEVTAILVHVMLDRARQFTSADLRDEKLVALGKLA
                     AGLAHELNNPASAMVRSAKALGDLLAQGEAAARALGALRLTESQLADLDAVQQTCLAA
                     PVRTWAPLERADREDALAA"
     gene            complement(721..993)
                     /locus_tag="AC16JC_07550"
     CDS             complement(721..993)
                     /locus_tag="AC16JC_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007718101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HIR5164586.1"
                     /translation="MCRNIKTLANFAPPATDEEIRASALQFVRKLSGTTHPSRANEAA
                     FHAAVEEVSATARRLIDSLTIHAPPRNREEEARKARERSARRFGTA"
     gene            1128..2549
                     /locus_tag="AC16JC_07555"
     CDS             1128..2549
                     /locus_tag="AC16JC_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR5164587.1"
                     /translation="MTEPAVSVAAPAGNTWWAVIRDAIRGVPHDYTAGSLNRAIVLLA
                     IPMVLEMAMESIFAVVDVFWVSHLGPDAVATVGLTESMLTLVYTAAMGLSIGVAAVVA
                     RRIGEKRPEAAAEAAVQGIAIGLVLAAVVATLGITLAPRLLSLMGATPAVTALGAGYT
                     RTMLGGSATVLLLFLINAIFRGAGDAAIAMRVLWLANAINIVLGPCLIFGLGPFPRLG
                     VTGAAVATTIGRGTGVLYQLYRLSRGDARVSVRQTHLALRPAVMTHLLRLSGSGTFQV
                     LVGTASYIGLVRIISTFGSAAVAGYTIAIRLVIFCLLPSWGLSNAAATLVGQSLGARN
                     PDRAEQAVWIAGRYNMVVLGVVGLVFVVLAGPIVSLFTQDPGAAPIAVLALRTMSYGF
                     VFYALGMVLTQAFNGAGDTWTPTWINLACFWLWEIPLAYVLARVLDLGPFGAFLAILL
                     GYSSLGLVSLVLFRRGRWKLRQV"
     gene            2584..3987
                     /locus_tag="AC16JC_07560"
     CDS             2584..3987
                     /locus_tag="AC16JC_07560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR5164588.1"
                     /translation="MTTPRQARALDRSIVEGPLPRAVWMLAWPTMVQNLIGGLQGMVD
                     HALVGHLVGYAGNAAIGVAIQIFIVVIVFVMSVFSGMGVLVARFAGSNEPEKVNRTVY
                     QAFLAAVALWGLVLAPLGWLLAPALLGVVHAAPAVRVEALPFLRIMFVGSLGSLFFFM
                     VSGALRAAGDPRTPLRLGIMLTVLNVVCNVALITGLGPLPRLGTAGAAVGTTVAGFVV
                     SGFAVYLLFSGSLPVLWHRGMDWRPDWGIIRALFRFGLPTGVQGVAMNVAGVLLLRFI
                     GSLAQSAQAQAAYAIGYTELFSFITWTSVGLLGAAATVAGQNLGAARPERAVRGVHIA
                     AGIGVALAATIGVLFLTIPRPLLGLFGMRDPGVVAVGTQLLAFLSVSGLFVSVALTYT
                     GGLQGTGDTRSPLYISIVSQIVVPLGLCTWWQATRGLHPVDVWTAILCGHIIRCALSV
                     LRFRQGKWRGISVDLGH"
     gene            4032..4388
                     /locus_tag="AC16JC_07565"
     CDS             4032..4388
                     /locus_tag="AC16JC_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020738162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5164589.1"
                     /translation="MVTSIARLDAVYSALGDGTRRAILTRLAAGDSTVGELAEPFAVS
                     RPAISKHLRVLERAGLVRRTRDGRVSRCRLDARPLAAAAGWIEYYRRFWDQSFDRLED
                     YLRDLQQRGKRHDRKN"
     gene            4372..4854
                     /locus_tag="AC16JC_07570"
     CDS             4372..4854
                     /locus_tag="AC16JC_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5164590.1"
                     /translation="MTARTKAARPPAQPQLVITRVLDAPRSLVFRVWTEPQHLVHWSC
                     PHGFTLTHCEGVLEPGGAWRSCMRSPEGGDLWLGGVYREVVVPERLVFTHAWDDANGR
                     RGRETIVIVTFTDEGGKTKMVFRQEGFASAEDRDGHAGGWNECFEKLAEHLKSGTVEG
                     "
     gene            4857..5471
                     /locus_tag="AC16JC_07575"
     CDS             4857..5471
                     /locus_tag="AC16JC_07575"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR5164591.1"
                     /translation="MGKIFVDVGMSLDGLIAGPNGRPGNPLGDGGMRIHDWMFGLASF
                     REHLQMPGGETGPDDDIVRATLARIGANVMGRRMFDEGEVGWPENAPFHTPVVVLTHK
                     PREPWVRPGGTTFYFVTDGFDRALEQARAAAGAGDVRISGGADTIRQALRAGVVEELH
                     LHLAPVLLGEGVRLFDGGRGLPEVEIMAVRPSLQVTHLSYRVVR"
     gene            5479..>5702
                     /locus_tag="AC16JC_07580"
     CDS             5479..>5702
                     /locus_tag="AC16JC_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016545743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR5164592.1"
                     /translation="MPPALDDTAGREIVATRVVDAPRDLVFRVWTEPEHIAHWWGPNG
                     FNTTIHEMDVRPGGVWRFVMRGPDGRDYQN"
BASE COUNT          747 a         2047 c         1929 g          979 t
ORIGIN      
        1 gcggccagcg cgtcctcgcg atccgctcgt tccagcgggg cccaggtgcg caccggcgcc
       61 gccaggcacg tctgctgcac ggcgtccagg tcggccagct gcgactccgt caggcgcagg
      121 gcgcccaggg cgcgtgccgc cgcttctccc tgggccagga gatcgcccag cgctttcgcg
      181 ctccgcacca tggccgatgc cggattattg agctcatggg ccagccccgc cgcgagcttt
      241 ccgagcgcca ccagcttctc gtcccgcaaa tcggccgagg tgaactggcg cgcgcgatcc
      301 agcatgacgt ggaccaggat ggccgtgacc tccgggcact cgcggatcat ctcggggaag
      361 cgatcgcggt ccacgagcca gatctcgctg tcggactcgg ccaccccgtc gccgatcgcc
      421 acggtgaccc gagagtacgg cagcacgccg ctcacctctc cgccgtgcca ctcggcgagc
      481 tttcgccggc cgcgaccccg gttcacatag agcaccacgt ggcccgtgag cacaaccatc
      541 aggccgttca taccggcgag ttgtccctgc cggatgggca tttcgcctgc ggcgtaccgt
      601 cgcatgtccc cattggcggc gagccattcc agctcctgca gcggggcgcc gccgagggtc
      661 gcgtgctcgc gcagccgctg cccgattgcc tgttgctccg tcgcggtcat atcgggcaca
      721 ctaggcggtc ccgaagcgcc gcgccgagcg ttcgcgggcc ttccgtgcct cttcctcgcg
      781 attgcggggg ggcgcatgga tcgtcagcga gtcgatcagc cgccgcgcgg tggcactgac
      841 ttcctctacg gcggcgtgga acgcggcctc gttggctcgc gaggggtgcg tagtcccgct
      901 gagcttgcgc acgaattgca gcgccgacgc acgaatctcc tcgtcggtcg ccggcggcgc
      961 gaaattggca agcgtcttga tgttccggca catgtcagag ttccaggatg gaaaccccga
     1021 caaatatacg gcctgattat cttcgggcgg attctccgcc ggtccaaccg cgatcgcttg
     1081 ggtccgcgaa cggcgcccgt acctacccct gcagagggtt gcagcgcatg accgaaccgg
     1141 ccgtgagcgt cgcggcacct gccggcaaca cctggtgggc ggtgatccgg gatgccattc
     1201 gcggcgttcc ccacgattac accgccggct ccctcaaccg cgcgatcgtg ctgctcgcca
     1261 tcccgatggt gctcgagatg gcgatggaat cgatcttcgc cgtggtagac gtgttctggg
     1321 tgtcccacct gggcccggac gccgtggcga cggtcggcct gacggaatcg atgctgaccc
     1381 tcgtgtacac cgccgcgatg ggactctcga tcggcgtcgc ggccgtggtg gcacgccgga
     1441 tcggggagaa gcggcccgag gccgccgcgg aggcggccgt ccaggggatc gccatcggac
     1501 tggtcctcgc cgccgtggtg gcgacgctcg gcatcaccct ggcaccacgg ctactgtccc
     1561 tcatgggcgc caccccggcc gtcaccgcgc tcggtgcggg ctacacgcgc acgatgctgg
     1621 gcggcagtgc cacggtgctg ctgctgttcc tcatcaacgc gatcttccgc ggggccggcg
     1681 acgcggccat cgcgatgcgc gtcctctggc tcgccaacgc gatcaacatc gtgctcggcc
     1741 cctgcctcat cttcgggctc ggcccgttcc cccggctcgg cgtcaccggg gccgcggtgg
     1801 cgaccacgat cggccgcggc acgggcgtcc tctatcagct ctaccggttg agccgcggcg
     1861 atgcgcgcgt gtccgtccgg cagacgcacc tcgccctgcg cccggcggtg atgacgcacc
     1921 tgctgcgcct gtcgggctcc ggcaccttcc aggtcctcgt cgggacggcg agctacatcg
     1981 gcctggtgcg catcatctcg acgttcggca gcgccgccgt cgcgggctac accatcgcga
     2041 tacggctcgt gatcttctgc ctgctgccgt cctggggcct gtcgaacgcc gcggccacgc
     2101 tcgtcgggca atcgctcggc gcgcgcaacc ccgaccgcgc cgagcaggcg gtgtggatcg
     2161 ccggccggta caacatggtg gtgctgggcg tcgtggggct ggtgttcgtc gtgctcgccg
     2221 gcccgatcgt cagcctcttc acgcaggatc ccggcgccgc tccgatcgcc gtcctcgcgc
     2281 tgcgcaccat gagctatgga ttcgtgttct acgcgctcgg catggtgctc acgcaggcgt
     2341 tcaacggcgc cggtgacacc tggacgccga cctggatcaa tctcgcctgc ttctggctgt
     2401 gggagatccc gctcgcctac gtgctcgcgc gcgttctcga cctcggtccc ttcggggcgt
     2461 tcctcgcgat cctcctcggg tactcctcgc tggggctcgt gagcctggtg ctgttccgcc
     2521 gcggccgctg gaagctgcgt caggtctgac gcgacggccg cttctgcccc tcgcacccga
     2581 tccatgacga ccccgcgcca ggccagggca ctcgaccgct ccatcgtcga aggaccgctg
     2641 ccccgcgccg tctggatgct cgcctggccc acgatggtgc aaaacctcat cggcggcctc
     2701 caggggatgg tggaccacgc cctggtcggc cacctcgtgg ggtatgccgg caacgccgcc
     2761 atcggcgtgg cgatccagat cttcatcgtg gtcatcgtgt tcgtcatgtc ggtgttcagc
     2821 gggatgggag tactggtcgc ccgcttcgcc gggtccaatg agcccgagaa ggtcaatcgc
     2881 accgtctatc aggcgttcct cgccgcggtg gccctgtggg ggctggttct ggcccccctg
     2941 ggctggctgc tcgcgccggc actgctcggc gtggtgcacg ccgcgcccgc ggtgcgcgtc
     3001 gaggctctgc ccttcctccg catcatgttc gtgggcagtc tcgggtcgct gttctttttc
     3061 atggtgagcg gagcgttgcg ggcggcaggc gatccgcgca ccccactgcg gctggggatc
     3121 atgctcaccg tgctgaacgt ggtgtgcaac gtcgccctga tcacgggcct cggccccctg
     3181 ccccggctgg gcaccgccgg cgcggccgtc ggcaccacgg tggccggatt tgtcgtgagc
     3241 ggattcgccg tctacctgct gttctccggc tcgctgcccg tgctgtggca tcgcggcatg
     3301 gactggcggc cagactgggg catcatccgg gcactgttcc gcttcgggtt gcccacgggc
     3361 gttcaggggg tcgcgatgaa cgtcgccggc gtgctgctgt tgcgcttcat cggctcgctc
     3421 gcccagagcg cacaggcgca ggcggcctat gcgatcggct acaccgagct cttctcattc
     3481 atcacgtgga cctcggtcgg gttgctgggc gcggccgcga cggtcgcggg acagaacctg
     3541 ggggcggccc gtccggaacg cgcggtgcgc ggcgtccaca tcgccgcggg gataggcgtc
     3601 gcgctcgccg ccacgatcgg cgtcctgttc ctcacgattc cccggcccct gctcgggttg
     3661 ttcgggatgc gcgacccggg cgtcgtcgcc gtggggaccc agctgctcgc cttcctgagc
     3721 gtgtccggcc tgttcgtgag cgtcgccctc acctacacgg gcgggctgca ggggacgggt
     3781 gacacgcgct cccccctgta catctcgatc gtgtcccaga tcgtggtgcc gctcggcctc
     3841 tgcacctggt ggcaggcgac ccgcggcctg cacccggtcg acgtgtggac agcgatcctg
     3901 tgcgggcaca tcatccgctg cgccctgagc gtgctgcgct tccgtcaggg gaagtggcgc
     3961 ggcatctcgg tcgacctggg ccactgatcc cgccttgaca gccggcgcgg gcgccgtata
     4021 ctggtaacca tatggttacc agtatagcgc gtctcgacgc cgtgtactcc gcgttgggcg
     4081 acggcacccg ccgggccatc ctgacccggc tcgccgccgg ggactccacg gtcggtgagc
     4141 tggccgagcc gttcgccgtc tcccgccccg ccatctccaa gcacctgcgg gtcctcgagc
     4201 gggcgggcct ggtgcggcgc acccgtgacg gccgcgtctc acgctgccgg ctcgacgccc
     4261 ggccgctcgc cgccgcggcc ggctggatcg agtactatcg ccgcttctgg gaccagagct
     4321 tcgatcggct ggaagactac ctgcgtgatc tgcaacagcg aggaaagcga catgaccgca
     4381 agaactaagg cggcgcgacc gcccgcccag ccgcagctcg tcatcacccg cgttctggac
     4441 gcgccgcgca gcctcgtctt ccgggtgtgg accgagccgc agcacctcgt ccactggtcc
     4501 tgcccccacg gcttcaccct gacccactgc gagggagtgc tcgaaccggg cggtgcgtgg
     4561 cggtcctgca tgcggtcccc tgaaggcggc gacctctggc tcggcggcgt gtaccgcgaa
     4621 gtcgtggtcc ccgagcggct cgtcttcacc cacgcctggg acgatgcgaa cggccgacgc
     4681 ggccgggaaa caatcgtgat cgtgacattc accgacgagg gcggaaagac gaagatggtt
     4741 ttccgccagg aaggcttcgc gtcggccgag gatcgtgacg gtcacgctgg cggctggaat
     4801 gaatgcttcg agaagctcgc cgagcacctg aagagcggaa cggtggaggg ttgacgatgg
     4861 gcaagatctt cgtcgacgtc ggcatgtccc tcgacggtct gatcgcgggc ccgaacgggc
     4921 gccccgggaa tcccctgggg gatggcggga tgcggatcca tgattggatg ttcggccttg
     4981 cgagcttccg cgagcatctc cagatgcccg gcggcgagac cggccccgac gacgacatcg
     5041 tccgggcgac cctggcgcgg atcggcgcca acgtcatggg ccggcgcatg ttcgacgagg
     5101 gcgaggtggg ctggcccgag aacgccccct ttcacacccc cgtcgtcgtc ctcacgcaca
     5161 aaccccgcga accatgggtc aggccgggcg gcaccacctt ctatttcgtg accgacggct
     5221 tcgaccgtgc gctggaacag gcgcgcgccg ccgccggcgc cggagacgtc cgcatttcgg
     5281 gcggcgccga cacgatccgg caggccctcc gggccggcgt cgtggaggag ctccacctgc
     5341 acctcgctcc cgtgctcctg ggcgagggcg tgcgcctgtt cgacggcggc cgcggcctgc
     5401 ccgaagtcga gatcatggcc gtccgtccct cgctccaggt gacgcacctg tcctaccgcg
     5461 tggtgcgata ggagaccgat gccccccgct ctcgacgaca ccgccggccg tgaaatcgtg
     5521 gccacgcgcg tggtcgatgc cccgcgcgac ctggtcttcc gtgtatggac cgagccggag
     5581 cacatcgcgc actggtgggg cccgaacgga ttcaacacca cgatccacga gatggacgtc
     5641 cggccgggcg gcgtctggcg cttcgtgatg cgcggacccg acggccgcga ttaccagaac
     5701 aa
//
LOCUS       DBNGZW010000319         4639 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1529737, whole genome shotgun sequence.
ACCESSION   DBNGZW010000319 DBNGZW010000000
VERSION     DBNGZW010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1529737"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..349)
                     /locus_tag="AC16JC_07585"
     CDS             complement(<1..349)
                     /locus_tag="AC16JC_07585"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR5164593.1"
                     /translation="MPDAGGGSSIEMWLSEHEDPGMDSALQYRVHEELRWESGLDPSG
                     ITVGVNARTVTLGGSVRTYPEKQAAELAACRVPRVQRVINNLCVNLAPSHAQPDDLLL
                     EEVTRVLEWDTLVP"
     gene            complement(492..1376)
                     /locus_tag="AC16JC_07590"
     CDS             complement(492..1376)
                     /locus_tag="AC16JC_07590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164594.1"
                     /translation="MNSRIRIVATAGLALVGALACQSKPSPQLQARIDSLQNAASERD
                     RLVQEMAQDARMISDVSAELAKVQVKGKLNVSSESPGQASRDSVVARVRYIATRLNDT
                     ESRLRSSERRIRGLTSVSDSLRNTLAATIANYDSVVSTQRTQLVAFATQIDSLKGENV
                     ALTAVNTALKDTVGDLTLRDNTVYYVIGTRDELKSRGLIDETGGSRFLFILWKSGKSI
                     QPARTLDPSAFTAIDKRQVTTIPLPDSSKTYQIASRQDLTALATPPDGDGKLHGSVQI
                     AAPAKFWSTSKYLIIVES"
     gene            complement(1555..2904)
                     /locus_tag="AC16JC_07595"
     CDS             complement(1555..2904)
                     /locus_tag="AC16JC_07595"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR5164595.1"
                     /translation="MLPPLLVLAILPASGASQARRSTELDASRRTAIVTAAARVAPGV
                     VSVSVLKRARSQPEAPFAFFFMPQDREVQGYGSGFIIGADGVIITNQHVTAGAQQIVV
                     TTRDGRDLPARLLGEDPQTDIAVVKVDATGLPTVPIGSSETLMIGEWVVAIGNPYAYL
                     LGNSEPSVTAGVVSAVGRNLLPSQDQAGVYVGMIQTDASINPGNSGGPLVNAAGEVVG
                     VNSNIFSNTGGSVGIGFAIPIERAIRVATELRRYGRVRRAWVGLDVSGSNELRGWKQA
                     GGLQVTTVAPGGPAAQAGMSAGDVLLGARGQRLRTFLDWDAVLLDAGPGDTLSVSCRC
                     GGRSRIIRLVVADLPTSSAEKVAVLGDVQVVTLTPAIRQERGVQVAQGVLIYDLGDDT
                     RRATGLAPGDVVFQVNRQRIASAAELRAAFAQAAGGQAITLWFERNGMIGRTSFYVQ"
     gene            complement(3004..4107)
                     /gene="solA"
                     /locus_tag="AC16JC_07600"
     CDS             complement(3004..4107)
                     /gene="solA"
                     /locus_tag="AC16JC_07600"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050131 - N-methyl-L-amino-acid oxidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HIR5164596.1"
                     /translation="MSFDIVIAGLGAVGSAAAYHLAGRGLRVLGLDRYHPPHALGSSH
                     GGSRVIRETAFEHPRYVPLARLAYENWRRLERDSGEALLHVTGVLFVGSLESPLVAGT
                     RASAREHGIPHELLEAGDIAHRFPALQRLDDAAGVFEPGGGWLDPERCVAASLRLAAE
                     RGVSLRFEEPVERWEPDGDGVVVTTPRDRYRARRLLLAAGPWMPELLGGAGVSLSVER
                     QVQHWFTAATAEAPRVPIFIRQVPEGVFYALPPAGEEIKVAVHHAGAATTPATVERHV
                     SPGEIDGVRALLERYAPALAGEHRRAAVCLYTNSPDGHFVIDRHPAHPAVVVASPCSG
                     FGFKFASAVGEILADLLQDRPSGVDVAPFRIGR"
     gene            complement(4247..4330)
                     /locus_tag="AC16JC_07605"
     tRNA            complement(4247..4330)
                     /locus_tag="AC16JC_07605"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4294..4296),aa:Val,seq:cac)
     gene            4417..>4639
                     /locus_tag="AC16JC_07610"
     CDS             4417..>4639
                     /locus_tag="AC16JC_07610"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR5164597.1"
                     /translation="MTPRESLIETSQPGSDERAVPSEQQRRALEAPIGPVLVLAGPGA
                     GKTFCLVERVRHLIEAHGARPARICAVTFT"
BASE COUNT          711 a         1640 c         1581 g          707 t
ORIGIN      
        1 tgggaaccag cgtgtcccac tccagcacgc gtgtgacctc ctccagcagc aggtcgtcgg
       61 gctgcgcgtg gctcggggcc agattgacac acaggttgtt gatcacccgc tgcacccgcg
      121 ggacccggca cgccgccagc tcggcggcct gcttctccgg gtacgtccgc accgagccgc
      181 ccagcgtaac ggtgcgcgcg ttgacgccca ccgtgatgcc cgagggatcg agcccactct
      241 cccagcgcag ctcctcgtgc acgcgatact ggagcgcgga atccatcccg ggatcttcat
      301 gctcgctgag ccacatctcg atcgaggaac cgccgcccgc gtctggcatg gtgtgcacct
      361 ccttccctat tcaactacgc ttcacgtgtg aacggggcgt gaagccgtca ggaaggaagc
      421 ttgttctccc cgaacgcacg aagccccggg cgcgaagccc ggggctcgtt ccggcgggga
      481 ctgctgccgg ctcagctttc gacgatgatc aggtacttcg atgtggacca gaacttggcc
      541 ggagccgcga tctgcacact gccgtgcagc ttgccgtcgc cgtcgggggg cgtggcgagc
      601 gcggtcaggt cctgccgcga cgcgatctgg taggtcttgg aggagtcggg cagcgggatc
      661 gtggtcacct gccgcttgtc gatcgccgtg aacgcgctgg gatcgagcgt ccgggcgggc
      721 tggatggact tccccgactt ccacaggatg aacaggaagc ggctgccgcc ggtctcgtcg
      781 atgaggccgc gggacttcag ctcgtccctc gtcccgatca cgtagtagac cgtgttgtca
      841 cgcaacgtca ggtcgcccac cgtgtccttc agggcggtgt tcacggccgt cagggccacg
      901 ttctcaccct tgagcgaatc gatctgcgtc gcgaacgcga ccagctgcgt ccgctgtgtg
      961 ctgacgaccg agtcgtagtt ggcgatggta gccgccagcg tgttacgcag gctgtcggag
     1021 accgacgtca acccgcggat gcggcgttcg ctcgaacgga gacgcgactc cgtgtcgttc
     1081 agccgcgtcg cgatgtagcg cacgcgcgcg acgacgctgt cccgggaggc ctgcccgggg
     1141 gactcgctcg acacgttgag cttgcccttc acctggacct tggccagctc cgcggagacg
     1201 tcggagatca tccgggcgtc ctgcgccatc tcctgcacca gccgatcgcg ctcgctggcc
     1261 gcgttctgca gcgaatcgat acgcgcctgc agctgcggcg agggcttcga ctggcacgcg
     1321 agcgcgccga ccagcgcgag accggcggtc gcgacaatgc gaatcctgct gttcatggga
     1381 catgtcctcc acaggggcac ttcggacggc cggaccggtg acgccccccg ccacctgtcc
     1441 gcgctgcgtg tgagcgatct caccaaccta acggtcaccc ggcgagcgac gccggatttc
     1501 atgaggcgcc aatatacccg gtaagttccc taacgtttcc ttcttggaca cccctcactg
     1561 cacgtagaac gacgtcctgc cgatcatccc gttgcgctcg aaccacagcg taatcgcctg
     1621 cccccccgcc gcttgggcga acgcggcgcg cagctcggcg gcggacgcga tgcgctgccg
     1681 gttcacctgg aacaccacgt ctcccggggc gagtcccgtg gcccggcgcg tgtcgtcccc
     1741 caggtcgtag atcagcaccc cctgcgccac ctggacgccg cgctcctggc ggatcgcggg
     1801 ggtgagcgtg accacctgga cgtcgccgag caccgcgacc ttctcggcgc tggacgtcgg
     1861 caggtcggcc accaccagcc ggatgatccg cgaccgtccg ccgcagcggc aggacacgga
     1921 caacgtgtca cccggaccgg cgtccagcaa taccgcgtcc caatcgagaa acgtgcggag
     1981 ccgctgcccg cgcgcgccga gcagcacgtc gccggccgac atccccgcct gggcggcggg
     2041 cccgcccggc gccaccgtgg tgacctgtaa cccgcccgcc tgcttccacc cacgcagctc
     2101 gttggacccc gacacgtcca gccccaccca ggcgcgccgc actctgccgt agcgtcgcag
     2161 ctcggtggct acgcggatcg cgcgctcgat gggtatcgcg aacccgatcc ccaccgagcc
     2221 gcccgtgttg gagaagatgt tgctgttgac gcccactact tcgcccgcgg cgttcacgag
     2281 cggccccccg gagttgccgg gattgatgga ggcatcggtc tggatcatcc cgacgtagac
     2341 gcccgcctgg tcctgggacg gtaacaggtt gcgtccgacc gcgctcacca ctccggccgt
     2401 gaccgacggc tcgctgttgc cgagcaggta cgcgtacggg ttgccgatcg ccaccaccca
     2461 ctcgccgatc atcagggtct cgctcgaccc gatgggcacc gtcggcaggc cggtcgcgtc
     2521 caccttcacg acggcgatgt cggtctgggg gtcctccccg agcaggcggg cggggaggtc
     2581 gcggccgtcg cgcgtcgtca cgacgatctg ctgcgcgccc gcggtgacgt gctggttcgt
     2641 gatgatgacg ccgtccgcgc cgatgatgaa ccccgagccg tatccctgta cctcgcggtc
     2701 ctggggcatg aagaagaagg cgaacggggc ctccggttgg gatcgtgccc gcttcagcac
     2761 gctcacgctc accactcccg gcgcgacccg ggccgccgcg gtgacgatgg cggtgcgccg
     2821 cgaggcgtcg agttcggtcg atctccgggc ctgcgacgcg ccgctggcgg ggaggatcgc
     2881 caggaccagg agcgggggaa gcatggtggc cttcatggca cgaccgtcga agctagcggg
     2941 aatcatgtga atcgtggatg aacccgagcg aatgatgccc caggggtccg ggccgtcgcg
     3001 ggctcaccgt ccgatccgga acggggcgac gtcgacgccc gacggccggt cctggaggag
     3061 atccgccagg atctccccca ccgcactcgc gaacttgaac ccgaagccgg agcaggggct
     3121 ggcgacgacc accgcggggt gcgccgggtg gcggtcgatc acgaaatgcc cgtcgggcga
     3181 gttcgtatag agacacaccg cggcgcgccg gtgctcgccg gccagggccg gcgcgtagcg
     3241 ctcgagcagc gcccgcacgc cgtcgatctc ccccggcgac acgtgacgtt cgaccgtggc
     3301 cggcgtcgtg gcggcgccgg cgtggtgcac tgcgaccttg atctcctcgc ccgccggggg
     3361 cagggcgtag aacacgcctt cggggacctg gcggatgaag atcgggactc gtggggcctc
     3421 cgccgtcgcc gccgtgaacc agtgctggac ctgccgctcg acgctgagcg atacgccggc
     3481 gccgccgagc agctcgggca tccagggtcc ggccgcgagg agcaaccggc gcgcccggta
     3541 gcgatcgcgg ggggtggtca ccacgacccc atcgccgtca ggctcccagc gctcgaccgg
     3601 ctcctcgaac cgcagtgaaa cgccccgttc ggccgccagg cggaggctcg ccgccacgca
     3661 gcgttcgggg tcgagccagc cgccgcccgg ctcgaacacg cccgccgcgt catcgaggcg
     3721 ctgcagggcg gggaagcgat gggcgatgtc gcctgcttcg agcagctcgt gcgggatgcc
     3781 gtgctcccgg gcgctcgccc gcgtcccggc gacgagcgga ctctcgagcg atccgacgaa
     3841 gagcacgccg gtgacgtgca acagcgcctc acccgaatcg cgctccagtc ggcgccagtt
     3901 ttcgtaggcc aggcgggcga gcgggacgta acgtgggtgc tcgaacgcgg tctcgcggat
     3961 gacgcgcgag ccgccgtgac tcgaccccag ggcgtgcggc gggtggtagc gatcgagccc
     4021 cagcacgcgc agtccgcggc cggcgaggtg gtaggcggcg gcgctgccga cggcgcccag
     4081 cccggcgatg acgatgtcga atgacacagc ggagcttacc ttccaagcga aaggccgaga
     4141 gcgtttgccc tcggccgttc ccgacgcagc gaagtgggct gcggagtcct cacaagtgca
     4201 cgaatgatgc tatgacgtcc ttccttggtc aagcagggta cggcggtgct ggcggcggga
     4261 ctcgaacccg caagggcttg cgcccagaga cgtgtgagga ctcagcgttt acccatttcg
     4321 ccacgccggc acctcatcgg ctgcgagcca gggtaatccc gagggccgtt gatgtgaggt
     4381 ctcacacgcg ccgtcgactg tgacgcgacg ataacgatga ctccccggga atccctcatc
     4441 gaaacatcgc agccgggatc cgatgagcgc gccgttccgt cggagcaaca acggcgcgcg
     4501 ttggaggcgc cgatcggacc ggtgctggtg ctcgccggcc ccggcgcggg gaagacattc
     4561 tgcctggtag aacgggtccg gcatctcatc gaagcgcacg gcgcacgtcc cgcccggatc
     4621 tgcgccgtca cgttcacca
//
LOCUS       DBNGZW010000320         2452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_915604, whole genome shotgun sequence.
ACCESSION   DBNGZW010000320 DBNGZW010000000
VERSION     DBNGZW010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_915604"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="AC16JC_07615"
     CDS             <1..454
                     /locus_tag="AC16JC_07615"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HIR5164598.1"
                     /translation="VTSTIATPAVQIPASHLDLLTRPICGVFTTMGADGQPQSSLVWV
                     DVDGEGLAGVNTTLERQKGRNILANPKTSLLVVDPDNTARFIQIRADAELVTDQALAL
                     EHLDALTRKYTSHPRYYGYIYPADQQARETRVICRLHARWITLDAIHA"
     gene            692..1600
                     /locus_tag="AC16JC_07620"
     CDS             692..1600
                     /locus_tag="AC16JC_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164599.1"
                     /translation="MPDAQRLGGVRIGPWIGPALCAGVALAWAWGHLAPNPEGPQEFA
                     PDWLPLIAAAIAAAAMLPHGGSPRWRRLQVALRWTGLLLMVWAANGLPFDVLTAAGLI
                     GHRTASGAIVLSSVYWPGLTTRALALGTVVVLGRTALGRPADAASTRPATWYAYAAFL
                     LALPYPVLRVHWALGGTLGLKSPGAAGEGWEPLLIAIPWVLAAILSLFLVSPPHWVSR
                     HLLLAAGWSATAVVAMIGPAAFWSFVSALASGSDTASGAIEFWVFGLFYGSWFLFAIA
                     AGAATRSYQLRSAAPPSAACATGVRA"
     gene            1864..>2452
                     /locus_tag="AC16JC_07625"
     CDS             1864..>2452
                     /locus_tag="AC16JC_07625"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR5164600.1"
                     /translation="MGDLAEASVPNAVASAGGTPGTTPPAPRLHWIDWLRVAAIAGVF
                     LYHSLRPFDATDWHVKNAETSAALGGVMAVFWSFGLAVLFLLSGAGARFALRRRTWRT
                     FLGERTTRLLVPFVAGTLLLSPVQGFIEATHKGEFAGSLVDYFGVWLGGLAGRLDEDL
                     LSPTFSGIGYHLWFLGFLFAMSVITLPLLEWLLGQS"
BASE COUNT          319 a          863 c          836 g          434 t
ORIGIN      
        1 agtgacgtcg acgatcgcga cgccggcggt gcagatcccg gcgtcgcacc tcgacctcct
       61 gacccgaccc atctgtgggg tgttcacgac gatgggagcc gacggtcagc cccagtcgag
      121 tctggtctgg gtggatgtcg acggcgaggg tctcgcgggg gtcaacacga cgctcgagcg
      181 ccagaagggc cggaacatcc tggccaatcc gaagacaagc ctcctcgttg tcgaccccga
      241 taacaccgct cgcttcatcc agatccgtgc cgacgccgaa ctcgtcaccg atcaagccct
      301 tgcgctcgaa cacctcgacg cgctgactcg gaagtacacg agtcatcccc gctactacgg
      361 ctacatctat cccgcggacc aacaagcgcg ggaaacacgg gtcatctgcc gcctccacgc
      421 ccgctggatc acgctcgacg caatccacgc ctgaggctcc gacagcagaa ggcgtcgcgg
      481 ctccggacat gcggccggcg tcactgcacg atccgcgtcg cacatgccaa cctgccccgg
      541 ctgcgccggc gcggggcacg aatctgacct cgtggagcga gcgcatcgga tcgcacggca
      601 cttttcggtg ccctggccct gagtggacac tttggcggca atgggtcggc ggctgcaaac
      661 ggaaggcggg gggctgtcac acccgcacac cgtgccggac gcacagagac tcgggggtgt
      721 caggatcggg ccgtggatcg gaccggccct gtgcgccggg gtagcgctgg cgtgggcctg
      781 gggacacctc gccccgaatc cggagggtcc ccaggagttc gctccggact ggctcccgct
      841 catcgccgcc gcgatcgccg cggcagcgat gttgccccat ggtggctcgc cgcggtggcg
      901 gcgcctgcag gtggcgctgc gctggaccgg tctgctgctg atggtctggg cggcgaacgg
      961 cctgccgttc gacgtgctca ccgcggcggg gctcatcggt caccgaacgg cgagcggcgc
     1021 gatcgtgctg tccagcgtct actggccggg gctgacgact cgggcgctgg ctctcggcac
     1081 cgtggttgtg ctcgggcgta ccgcccttgg acgcccagcc gacgcggcct ccacccggcc
     1141 ggccacctgg tacgcctacg ctgcgttcct gcttgccctg ccgtacccgg tcctcagggt
     1201 gcactgggcg ctcggcggaa ccctcgggct caagtcgccc ggcgcggctg gcgaaggctg
     1261 ggaaccattg ctgatcgcga tcccatgggt gctggccgcc atcctgtcgt tgttcctcgt
     1321 ttcgccgccg cactgggtct cgcgccactt gttgctggcc gcgggctggt ccgccaccgc
     1381 cgtcgtcgcc atgatcgggc cggcggcctt ctggtcgttc gtcagcgcgc tcgcctccgg
     1441 cagcgatacc gcctcgggtg cgattgagtt ctgggtcttc ggtctcttct acggcagctg
     1501 gttcctgttc gccatcgccg ccggggctgc cacccgctcg tatcagttgc gcagtgcagc
     1561 tcctccctcg gctgcctgcg caaccggcgt tcgggcgtaa cggcactggc ggagtgtccg
     1621 cctgggtgtg agattcggtc ctgccggtga cgtggcgacg ggaggaggtc gcccagatgc
     1681 atagcgtgtc ggctcaggca cgccgtccgg tcttggcgat gggccgcgac catgcgcgcg
     1741 cggcccgaag ccgggcggcg atgtgcctct ggctgcggcg gggctccacc gtagccttcg
     1801 gcccactggc cgcctctctc ggccggattt cgttggcacc gatacaccgt tcggaagcgc
     1861 gcgatgggcg acctggctga ggcgtccgtt ccaaacgcgg tggcgagcgc cggcggaacg
     1921 cccgggacca ccccgcccgc accacggctg cactggatcg actggctgcg cgtcgcagcg
     1981 atcgccggcg tgttcctgta tcacagcttg cgtcccttcg atgccaccga ctggcacgtg
     2041 aagaacgcgg agacgagcgc cgccctgggc ggcgtgatgg cggtcttctg gtcgttcgga
     2101 ctcgccgtgc tgttcctgct gtcgggcgcg ggagcccggt ttgccctccg ccggcgaacc
     2161 tggcggacgt tcctcggcga gcgaacgacg cggctcctcg tgccgttcgt ggccggcacc
     2221 ctgctcctct cgccggtgca ggggttcatc gaggcaaccc acaagggcga attcgccggg
     2281 tcgttggtcg actacttcgg agtatggctc ggtgggctgg ccggccgtct cgacgaggat
     2341 ctcctgtccc cgacgttctc agggatcggc taccacctgt ggttcctcgg cttcctgttc
     2401 gcgatgtccg tcatcaccct gcccctgctc gaatggctgc tcggacagag cg
//
LOCUS       DBNGZW010000321         4191 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_430270, whole genome shotgun sequence.
ACCESSION   DBNGZW010000321 DBNGZW010000000
VERSION     DBNGZW010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4191)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4191)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4191
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_430270"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            202..891
                     /locus_tag="AC16JC_07630"
     CDS             202..891
                     /locus_tag="AC16JC_07630"
                     /inference="COORDINATES: protein motif:HMM:NF040120.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HIR5164601.1"
                     /translation="MSAEPMSAVPPARRWPISSVVLIVANVVPLLGVLTHHWTVFAVV
                     LLYWCENVVVGVFNVLRMLWARPQDPVAQAGKIFLIPFFCVHYGGFAFGHGVFVFALF
                     GGDFKQGGLGFNPHIVLKAVRQFGLGWAVAALFASHGFSFLHNYLAGGEYRRAGLSQL
                     MTQPYARVMVLHVAIIAGAFLVQALGSAVPALALLIVLKTVIDLGAHTAERRRLGTLP
                     PDQAVLATTSS"
     gene            complement(861..3500)
                     /locus_tag="AC16JC_07635"
     CDS             complement(861..3500)
                     /locus_tag="AC16JC_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="coiled-coil domain-containing protein"
                     /protein_id="HIR5164602.1"
                     /translation="MAVSLNKFFGHQSDTADLPTELRAILSQMRQERAAFEALHTRAQ
                     ESAQQLNQIVQPLEETRRSVSEVQSRVKALERVVPVLATLDEQTEGVAKLQRRMETQL
                     GHSSDDAKRLRGEIQELRGQLEQALALKSELGAFLERAAELSKLRGDTDAALTQLREV
                     TQSYDRVRERQEELRRASESAAQRLNAFEERQQDAQGGVVAAESRVAGLERTLTHLTQ
                     VAADAAQSARQLGSLKSLTDAVSQKVTAIEQQREVVERTSAQMTRLLELDQELEERMR
                     AQEEGTRRLGAMEAKLAELQVAHEAVQSGSAQMQTQHEELRRGDEELRGRLATLREDV
                     ERTVKRFELENQGLDSVGQRIQDLRGGLTDLEGRVRSLDEASRRISEVQARTEGLRVQ
                     LDDVAEDVGELEQQAERVRAIEATAVRLQETVEGVAQRTTRLEQSKPVVEAVLHDVAS
                     LRGTHETVKDALEQLQTAEADLARAREGQAGTKAWLVGVTASVDALREELEAVEDLRP
                     TVEVVKTEAGRLAQSVGELEARRQLVEELNAKLADLAGREAQIEERAHTLLGRLNGAD
                     ERFLALAAHAEEAGRIERLVPAAVATVERVERRVADVDATLASVEARTQSVEHLADRT
                     SALGQELDRRQAALDKAAEHLAEASRLREQAANAAQQLEERSERLGSALGTAGSRLGE
                     ITTALDELDGRAGGLRLVQKRMSQFEERLTRWEVLEASLTRGLEQVVDRRSTLDALAA
                     DMQRLFDMAERTTKDVRAIADAKEEITVTRQTLERIVSLAGDVQEAAGTLDHRKRQIE
                     QAEERLGRVETLLADVQSNLELLHGQKAVVEQVSGTVASLELHAKRAEALIDTLREER
                     DVTDQVREALAQLRQEEVVAKTA"
     gene            complement(3846..>4191)
                     /locus_tag="AC16JC_07640"
     CDS             complement(3846..>4191)
                     /locus_tag="AC16JC_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164603.1"
                     /translation="MEGLMTWAGGSAGLDFHDLLPDLAAYVVAHPGLTLRGIMDAPPP
                     RVGTLDVGYDGFAVLCDMIHGVGGVAAVRQWLQSGTAADSVLGAAARLLHLRADQLDR
                     AWRRRVESAGRR"
BASE COUNT          545 a         1535 c         1389 g          722 t
ORIGIN      
        1 gctgcaggct gtcgaggaac gcgccgacct gggcgtgcgt ggacgtggct gcgaagttgg
       61 tgcgctcggg agtggttcgg ggcagctgct gggcggcgag tggagatgcc gccaacggca
      121 ggaagcagag ggtctggcgc agaagccacc gcatcgtcat cctcgtgggc cggaatccgg
      181 cgaatctacg tggaggcgac gttgtccgct gagcccatga gcgcagtccc accagcacgc
      241 cgctggccca tctcatccgt ggtgctcatc gtcgccaatg tcgtcccgct gctcggcgtc
      301 ctgacccacc actggaccgt gttcgcggtg gtgctgctct attggtgcga gaatgtggta
      361 gtgggcgtgt tcaacgtgtt acggatgttg tgggccaggc ctcaggaccc ggtggcgcag
      421 gcggggaaga tctttctcat tccgttcttc tgcgtgcact acggcggatt cgccttcggg
      481 cacggggtct tcgtgttcgc cctgttcggc ggcgatttca agcagggcgg gctcgggttc
      541 aacccgcaca tcgtactcaa ggcggtcagg cagttcgggc tcggctgggc cgtggcggcg
      601 ctgttcgcga gccacgggtt ctccttcctt cataactacc tggccggggg cgagtaccgc
      661 cgggcggggt tgagtcagct catgacgcaa ccctacgccc gggtgatggt gctccacgtc
      721 gctatcatcg ccggggcctt cctcgtccag gccctcggct ccgccgtgcc ggccctcgcg
      781 ctgctcatcg tcctcaagac cgtgatcgat cttggcgccc acacggccga acggcgcagg
      841 ctcggcacgc tgcctcccga tcaggccgtc ttagcgacga cctcctcctg acggagctgg
      901 gccagggcct cgcgcacctg gtcggtgacg tcccgctcct cgcgcagcgt atcgatcagc
      961 gcttcggccc gcttcgcgtg cagctccagc gacgccacgg ttccgctgac ctgctccacc
     1021 acggccttct ggccgtgcag cagctcgaga ttcgactgga cgtcggcgag cagcgtctcc
     1081 acccgcccga gccgttcctc ggcctgctcg atctgccgct tgcggtggtc gagcgtgccg
     1141 gcggcttcct gcacgtcccc ggcgagcgac acgatgcgct cgagggtctg gcgcgtgacg
     1201 gtgatctcct ccttggcatc cgcgatggca cgcacgtcct tggtcgtccg ctcggccata
     1261 tcgaagaggc gctgcatgtc cgctgccagc gcgtcgagcg tcgagcgccg atcgaccacc
     1321 tgctccagac cgcgggtgag ggacgcctcc agcacctccc acctggtcag ccgctcctcg
     1381 aactgcgaca tccgcttctg taccagccgg agtccgccgg cacgcccatc gagctcgtcc
     1441 agcgccgtcg tgatctcgcc cagccggctc cccgccgtcc cgagcgcact gcccaggcgc
     1501 tcgctccgtt cttcgagctg ctgcgccgcg ttggcggcct gctcccgcag gcgagacgcc
     1561 tccgccaggt gctcggcggc cttgtcgagc gccgcctgac ggcgatcgag ctcctgaccc
     1621 agcgcgctgg tgcgatccgc cagatgttcc acgctctgcg tgcgtgcctc gaccgacgcg
     1681 agcgtcgcat ccacgtccgc cacgcgccgc tcgacccgct ccaccgtcgc gaccgcggcc
     1741 gggaccagcc gctcgatgcg tcccgcttcc tcggcgtgcg ccgccagggc gaggaaccgc
     1801 tcgtcggccc cgttcaaccg gccgagcagc gtgtgggcac gctcctcgat ctgcgcctcg
     1861 cggccggcga ggtcggcgag cttcgcgttg agctcctcca ccagctgccg gcgcgcctcc
     1921 aactcgccta ccgactgggc cagtcggccc gcctcggtct tgaccacctc gaccgtggga
     1981 cgcaggtcct cgaccgcctc gagctcctcc cgcagggcgt ctaccgatgc ggtcacgccg
     2041 accagccatg ctttcgtacc ggcctggccc tcgcgggccc gtgcgaggtc ggcttcggcc
     2101 gtttgcagct gctcgagcgc gtccttgacg gtttcatgcg tgccccgcaa gctcgcgacg
     2161 tcgtgcagca ccgcctcgac caccggcttc gactgctcca ggcgcgtcgt gcgttgggcc
     2221 acgccctcca ccgtctcctg gagccggacg gcggtggcct cgatggcccg cacgcgctcg
     2281 gcctgctgct ccagctcccc gacgtcctcg gccacatcgt ccagctggac ccgcagcccc
     2341 tcggtccgcg cctgcacctc ggagatccgg cggctcgcct cgtccagcga ccgcacgcga
     2401 ccttccaggt ccgtcaggcc gccgcgcaga tcctggatgc gctgccccac ggagtcgagg
     2461 ccctggttct ccagctcgaa gcgtttcacg gtgcgctcca cgtcctcccg cagcgtggcg
     2521 agacggccgc gcagttcctc gtctccgcgc ctgagctcct cgtgctgcgt ctgcatctgc
     2581 gccgagcccg actggaccgc ctcatgggcg acctgcagct cggcgagctt cgcttccatc
     2641 gcgccgagac gcctggtgcc ctcttcctgg gcgcgcatcc gctcttcgag ctcctggtcg
     2701 agctcgagca gccgcgtcat ctgggcggag gtgcgctcca cgacttcgcg ctgctgctcg
     2761 atggccgtga ccttctggga gaccgcatcg gtgagggatt tcagggatcc gagctggcgc
     2821 gcactctgcg ccgcgtccgc ggccacctgc gtcaggtggg tgagggtacg ctccaggccg
     2881 gcgacgcgcg actcggcggc gaccacgccg ccctgagcgt cctgttgccg ttcctcgaac
     2941 gcgttgagcc gctgcgcggc cgactcgctc gcgcgccgca gctcctcctg gcgctcgcgc
     3001 acccggtcgt agctctgcgt cacttcgcgg agctgcgtca gggccgcgtc cgtgtcaccg
     3061 cgaagcttgc tcagctccgc ggcgcgctcc agaaaggcgc ccagctcgct cttgagcgcg
     3121 agcgcctgct cgagctgacc gcgcagctcc tggatctcgc cgcgcaaccg cttggcatcg
     3181 tcggaggagt gtcccagctg cgtctccatg cgccgctgca gcttcgcgac cccttcggtc
     3241 tgctcgtcga gtgtggccag cacggggacc acccgttcga gcgccttgac gcgcgattgc
     3301 acctcgctca ccgaccggcg cgtctcttcc agcggctgga cgatctgatt gagctgctgg
     3361 gcggattcct gcgcgcgggt atgcagcgcc tcgaaggcgg cgcgctcctg gcgcatctgc
     3421 gacagaatgg cgcgcagctc cgtgggcaga tcggcggtgt cggattgatg accgaagaac
     3481 ttgttgagcg agacggccat ggcgcgaatc cccgggcaat gggtcgttgc ggcggcgctg
     3541 ctgggagggc gctggtcagc accacgggcg ctggccggca ccgggtgagg caggaacgca
     3601 acggacatgc cgttccgtgg gaggagttgc tactgcttgg ggtgattgaa gttagacgat
     3661 tgggcggtgg cctgcgccgt ctcacgctct cgcctctcgc aggcgaaact gcaaaatacg
     3721 gcaaaaaccc taggggtggg cgtacctccg gatgtgctcc cgccagccct caacgttcat
     3781 tggccaggag cgggagaaca ccgtcgaaga cggccatggc ggcgtaggac tcagggggcg
     3841 ccagctcagc gacggccggc cgactcgacg cgacggcgcc aggccctgtc gagttgatcg
     3901 gctcgaaggt ggaggagccg cgcagccgcg cccaggaccg agtcggcagc cgtccccgac
     3961 tgcagccact ggcgcaccgc cgccactccg ccgacaccat ggatcatgtc gcacagcacc
     4021 gcgaagccgt cgtagccgac gtccagtgta ccgacgcgcg gcggcggcgc gtccatgatg
     4081 ccccggaggg tgagccccgg atgcgccacg acgtaggccg ccaggtcggg caggaggtca
     4141 tggaaatcga ggcctgccga gcccccggcc cacgtcatca gtccttccat c
//
LOCUS       DBNGZW010000322         9394 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_981355, whole genome shotgun sequence.
ACCESSION   DBNGZW010000322 DBNGZW010000000
VERSION     DBNGZW010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9394)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9394
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_981355"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1359)
                     /locus_tag="AC16JC_07645"
     CDS             complement(145..1359)
                     /locus_tag="AC16JC_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164604.1"
                     /translation="MSAATNAVRTELAQKARAFPVGSFRAIGLGATAVGLVLLLLSLR
                     GHADRVWAAYHVNFIFWLGLAEGGVIVAASQKLAKGHWSGLVIRFAEASVAFLPVAVV
                     LFAASWFGRGYIYPWVAEPRTDLGWWLQPVWFYVRDTAVLVLMAWLSWRFVRSDLAPD
                     LAELAGGTPVDPSPEARGLITRDAALAALAFAFGLSVIAFDTVMSLAHRWVSNLYGAF
                     YFMGSFLTALMWLAILSVRMRRRMNLGDLYSRRQQHDLAKLCFGFTVFWAYLMFAQFL
                     VIWYGNLPEETYFIFHRLWGAWRPVGVAVFLMVFLIPFIGLLGVRPKRSSAWLLGVAL
                     VSVAGVWLERFFEIIPSLNGGAPAAFGLPELGVLVFFGGLYVLAYSWFAARYPMISPR
                     LAADTLAREHGH"
     gene            complement(1356..1898)
                     /locus_tag="AC16JC_07650"
     CDS             complement(1356..1898)
                     /locus_tag="AC16JC_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5164605.1"
                     /translation="MHRRLLLIGVALLLTACDPDNVVHHVGWFATMRHQRSIRPYTMP
                     RGTVPGTVPITGPEPDSVGLAVADRLDNPRTRTAESLNRGKWVYETYCLICHGDAGRG
                     DGPISALAGGPFFGVRSLVTDTVARRTDGYLYGVLVHAPSMGRGLMPVYGDKIHGADR
                     WDVVNYVRSLQQQARLAGRL"
     gene            complement(1891..2436)
                     /locus_tag="AC16JC_07655"
     CDS             complement(1891..2436)
                     /locus_tag="AC16JC_07655"
                     /inference="COORDINATES: protein motif:HMM:NF023249.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HIR5164606.1"
                     /translation="MMPSVLRDIFHPPQTPGVLAVFGHLDATVDAIGQLKAGGHGDDL
                     TVYSPIPRHELEAVLDQPTSPVRMWTLIGGMTGCGIGAWLTLYMSYDWPVVVGGKPVG
                     SILPYVIIMFEMTVLFGALTTILGLVLNALLASRHQGAIAYDPRFTNDKFGIFVRCAP
                     DKAASLEGLLKGAGAEEVRRA"
     gene            complement(2433..3785)
                     /gene="nrfD"
                     /locus_tag="AC16JC_07660"
     CDS             complement(2433..3785)
                     /gene="nrfD"
                     /locus_tag="AC16JC_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR5164607.1"
                     /translation="MADTGTPASRVTYDFVTRAIARTLGNPGKGYYTLLAAAVSLLAV
                     GIVCLLFLLRYGLGLAGYSHPVYWAVFITCFVFWVGIAHSGTLISAILYLFRSGWRTA
                     VYRTAEAMTVFAVMTAGLFPLIHIGRQWYFYWLIPYPNERGLWPNFKSPLIWDEFAIG
                     TYFTVSTVFLIMGLIPDIAAVRDVATGWRKKLYAITGLGWRGTNEEWRHFTRAYLYLA
                     ALATPLVLSVHSVVSWDFAMSIVPGWHATLFAPYFVAGAIYSGVAMVITLLVPIRRLF
                     HLEEIITVHHFENLAKLCLFTGLIVSFSYCTENFVAWFGGNPDERAGFWFRAFGSFWW
                     ASWTMNICNGLLPWLLWFKKVRTNITGLFVISIFVNIGMWFERFVIIVVSLAGEPYSG
                     WTNGGYNPTWADWGIMAGSFGWFFMWFLLFVKNFPAVSISEVKEVLVPPQLAKGRVPR
                     "
     gene            complement(3789..6767)
                     /locus_tag="AC16JC_07665"
     CDS             complement(3789..6767)
                     /locus_tag="AC16JC_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR5164608.1"
                     /translation="MNRRRFLKVLGVTGGGAAVLSGCGIGPEPTEKLIPYVVPPEGQT
                     PGIATYYASTCRECAAGCGIQVKVREGRALKIEGNPASPVNHGRLCARGQAALQGLYN
                     PDRVTGPMRRNAAGQFEPVSWDDAITMLRSKVAEARDRGLVFVTGLESGSFGDLVDEW
                     TKEAGGRRVTCEPFGFEALREGNRLAFGDASVPAYDFAAAGYILSFGADFMETWLSPV
                     GFQNAFTHAHSFESGTSSGMAKFAYVGPRMPQTALAADEWLPVAPGTEGLLALAMAQV
                     IVRQRLAAVPADAARLSLPAPASVAQAVGLDAETIERLAREFVAAGAGLAVAGGMAAH
                     YPNGAEIVAAVNILNYVAGAVGKTVKFGADLAPGSGAAGSHRELSELTADMAAGNVAV
                     LLVHGANPVYALGGAFAQAWGKVPFKVSFSSYLDETAAGADLVLPDHHPLEQWSDSLP
                     RAGVYALQQPAMLPVFDGTMQTGDVLLRAAGKPGSFKTSLQGKWQALHQRLAGPRGPG
                     FDQWWTDALQRGGVYGDAPARSVRLAAAAAGVAAAAPSFTGDAVAVIFPHPALHDGRG
                     ANKPWLQELPDPVSKLTWHGWVEVHPDTAAKWQLASGDVLLVKSAAGGVNAPVWITPS
                     VRPGVVALPTGQGHTAYGRYAQNRSFKAFDLLDVAANDYGGRTHVVAVTVAKTTDHRR
                     LASTEGTGRQLPEDVVPVLSLERARGLHAGEHAIEAEGVPEYARAAEEGWAEAQHEKA
                     SLGNYAGDHPRWAMAIDVGKCTGCSACVTACYAENNVATVGEDLVVRGREMAWLRIER
                     YFRPTVDGASVSAVTSPMLCQQCGNAPCEPVCPVFAAYHTTDGLNGQIYNRCVGTRYC
                     SNNCPYKVRVFNWYNYAEPGGHWEAFAAPLNWQLNPDVTVREKGVMEKCTFCVQRIRG
                     AQNAARMEDRTVRDGDIVPACAQTCPSEAIVFGDLNDPRSRVAQLAEDPRGYHVLARL
                     NTKPGVTYLAAVRAGAGEEA"
     gene            complement(6799..7350)
                     /locus_tag="AC16JC_07670"
     CDS             complement(6799..7350)
                     /locus_tag="AC16JC_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5164609.1"
                     /translation="MSIFLVMGAVPLAAQGAVPAPGTAGYKAPVITDTATLAGPRQPI
                     FFRHDIHAGQDRIPCLYCHNTVTVSSEPGMPALRTCIGCHQIIGGSTPSHQAEIAKVR
                     KLWSDRKPPEWIRVYWVPGFVHSPHMRHIKVLGNTPDACANCHGAVNGTTQISQVKTL
                     KMGWCIQCHMERKLSRDCSVCHY"
     gene            complement(7484..7945)
                     /locus_tag="AC16JC_07675"
     CDS             complement(7484..7945)
                     /locus_tag="AC16JC_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164610.1"
                     /translation="MSRLPNGYWATNLYMSPRARPKSDAPARDLPKLAAGRFKRAREN
                     IRALRHVTEQIVYMGTEWKWVWMYEVGGRKLGFLHPMESGVSGTFVLNDAEEQELVLA
                     DGLPRSVKQAVRDGRLVEGVRWCWMDFPDFGTVDGFVGVIQLKHHLLSRPD"
     gene            complement(8071..9210)
                     /gene="menC"
                     /locus_tag="AC16JC_07680"
     CDS             complement(8071..9210)
                     /gene="menC"
                     /locus_tag="AC16JC_07680"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HIR5164611.1"
                     /translation="MLVSRAELFELTLPLVEPFIISGGAIRERRSLIVALHDATGHTG
                     YGESPPFELPFYSEETLAGAGDLIARVLLPRVVGREFADPGEIDAVLRVGVRGNPFAR
                     AGIETAAWDLEADRRGTGLAALLGERLETSRMPAIPCGVALGIPEDRRPATLARWVGA
                     ALELGYRRVKIKVMPGWDEGAVRAARDAMAGVDLPLTVDANGAYEWPGHERDLRRLDE
                     TGLLYIEQPLAPEELVGHAQLGQTLRTPICLDETLKSASHARQIVELAGPRVWNIKVH
                     RVGGLTEVCRIYRIAESYGAELWAGTMPESGIGSQAPLAVAALPRCVYPSDLEPSARW
                     YGPDQDVIELTMGRDGRMAVPEQSGRGLLDAARFGAVTRSLMVLP"
     gene            complement(9191..>9394)
                     /locus_tag="AC16JC_07685"
     CDS             complement(9191..>9394)
                     /locus_tag="AC16JC_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164612.1"
                     /translation="LHTFFQLPLDEYSARSGSRTQRALHQLVFHPQSGLLAELWRLEQ
                     QGAIHRRGGELHFLDLPHARLKG"
BASE COUNT         1599 a         3279 c         3189 g         1327 t
ORIGIN      
        1 aggaccaccc gatgacgatc accgccggga tcgcggcggt ggtgaggaac cgagctcacc
       61 tgaggggcac gcctccgcgg gagcccaggg tcccggaatt caacaccgtg tccggcggtt
      121 cgcgccgggc gccgcggccg cgaactagtg tccgtgctcc cgggccaggg tgtccgccgc
      181 caggcggggg gagatcatcg ggtagcgcgc ggcgaaccag ctgtacgcca ggacgtacag
      241 gccgccgaag aagaccagga cgccgagctc cggcagcccg aacgcggccg gggccccgcc
      301 gttcagcgac ggaatgatct cgaagaaccg ctccagccag acgccagcga cgctcaccag
      361 ggcgacgccg agcagccacg cggaggaccg tttgggccgc acgccgagca ggccgatgaa
      421 cgggatcagg aagaccatga ggaacacggc gacgccgacg ggccgccacg ccccccacag
      481 ccggtgaaag atgaagtacg tctcttcggg gaggttgccg taccagatca ccaggaactg
      541 ggcgaacatg aggtaggccc agaacacggt gaagccgaag cacagcttgg ccaggtcgtg
      601 ctgctgccgg cgggagtaga ggtcgcccag gttcatccgc cggcgcatcc ggacgctcag
      661 gatcgcgagc cacatcaggg cggtcaggaa gctccccatg aaatagaacg cgccgtacag
      721 gttgctcacc cagcggtgcg cgagcgacat gacggtatcg aacgcgatga cgctcaggcc
      781 gaacgcgaag gcgagggccg ccagggcggc gtcgcgcgtg atcaggccac gcgcttccgg
      841 agacgggtcc accggcgtgc cgccggcgag ctcggccaga tcgggagcca gatcggaacg
      901 tacgaatcgc cacgacagcc aggccatgag gacgagcacg gcagtgtcgc gcacgtagaa
      961 ccacaccggc tgcagccacc agccgaggtc ggtgcggggc tcggcgaccc aggggtagat
     1021 gtaaccgcgc ccgaaccagg aggcggcgaa cagcacgact gcgacgggga gaaaagcgac
     1081 cgaggcttcc gcgaaccgga tcacgagccc cgaccagtgc cccttggcga gcttctgcga
     1141 cgccgcgacg atgacgccgc cctcggcaag gccgagccag aagatgaaat tgacgtggta
     1201 ggcggcccag acccggtcgg cgtgaccccg gagactcagc agcagcagga ccaggccgac
     1261 cgcggtggca cccagaccga tcgcgcggaa cgatcccacg ggaaacgcgc gcgccttctg
     1321 cgccagctcg gtgcgcacgg cgttggtggc ggcgctcaca ggcggcccgc cagacgggcc
     1381 tgctgctgca gggaccgcac gtagttgacg acgtcccagc ggtcggcacc gtggatcttg
     1441 tcgccataga ccggcatcag gccgcgcccc atgctggggg catggaccag cacgccgtac
     1501 aggtacccgt cggtgcgccg ggccacggtg tcggtgacga gcgagcgcac accgaagaag
     1561 gggccgcccg ccaacgccga gatcggcccg tcacccctcc ccgcgtcgcc gtgacagatg
     1621 agacagtagg tctcgtacac ccatttgccg cggttgaggg attcggccgt gcgcgtgcgg
     1681 gggttgtcca gccggtccgc gaccgcgagg ccgaccgagt cgggctcggg gccggtgatg
     1741 ggaaccgttc cggggacggt gccgcgcggc atggtatacg gtcggatgct gcgctggtgg
     1801 cgcatggtgg cgaaccagcc tacgtgatgc accacgttgt ccggatcgca cgccgtcagc
     1861 agcagcgcca cgccgatgag gaggagccgc ctatgcacgg cggacctcct cggccccggc
     1921 gcccttcagc aggccttcca gcgaggcggc cttgtccggc gcgcagcgca cgaagatccc
     1981 gaacttgtcg ttggtgaacc gcgggtcgta ggcgatggcc ccctgatgcc gggacgcgag
     2041 cagcgcgttc aggacgagcc ccaggatcgt ggtgagggcg ccgaacagca cggtcatctc
     2101 gaacatgatg atgacgtagg ggagaatcga gcccacgggt ttgccgccca ccacgacggg
     2161 ccagtcatag gacatgtaca gcgtcagcca ggcgccgatg ccgcatccgg tcatgccgcc
     2221 gatgagcgtc cacatgcgga ccgggctggt gggttgatcg agcaccgcct cgagctcgtg
     2281 gcgcggaatc ggcgagtaca cggtgagatc gtcgccgtgg ccgcccgcct tcagctgccc
     2341 gatcgcgtcg accgtggcat cgaggtgacc gaagaccgcc agcacgcccg gcgtctgcgg
     2401 aggatggaag atgtcccgga gcacgctggg catcatcgtg gcaccctccc cttggcgagc
     2461 tggggcggca ccagcacttc cttcacttcc gaaatcgaca ccgccgggaa gttcttcacg
     2521 aacaggagga accacatgaa gaaccagccg aagctcccgg ccatgatccc ccagtcggcc
     2581 caggtcgggt tatagccgcc gttggtccag cccgagtagg gctcgccggc gagcgacacc
     2641 acgatgatca cgaagcgctc gaaccacatc ccgatgttca cgaagatcga gatgacgaac
     2701 agtccggtga tgttggtgcg caccttcttg aaccacagca gccacggcag caggccgttg
     2761 cagatattca tggtccagct ggcccaccag aacgagccga aggcgcggaa ccagaagccg
     2821 gcccgctcgt cgggattgcc gccgaaccag gcgacgaagt tctcggtgca gtaggagaag
     2881 ctgacgatca ggccggtgaa caggcacagc ttcgccagat tctcgaagtg gtgcaccgtg
     2941 atgatttcct cgaggtggaa cagccgccgg atcggcacga gcagcgtgat gaccatcgct
     3001 acgcccgagt agatggcccc ggccacgaag tacggcgcga acagcgtcgc gtgccagccg
     3061 gggacgatgg acatcgcgaa gtcccacgac accacgctgt gcaccgacag cacgagcggc
     3121 gtcgcgagcg ccgcgaggta caggtacgcc cgcgtgaagt gccgccactc ctcgttggtg
     3181 ccgcgccacc ccaatcccgt gatcgcgtac agcttcttgc gccagccggt ggccacgtcc
     3241 cgcaccgccg cgatgtcggg gatcagcccc atgatcagga acacggtgga caccgtgaag
     3301 tacgtcccga tcgcgaactc gtcccagatg agcggtgact tgaagttggg ccacagcccc
     3361 cgctcgttcg gatacggaat caaccagtag aagtaccact gccgcccgat gtggatgagc
     3421 ggaaacaggc ccgccgtcat cacggcgaac accgtcatcg cctcggcggt gcggtatacc
     3481 gccgtgcgcc agccggaccg gaacaggtac aggatcgcgg agatcagggt gcccgaatgc
     3541 gcgatgccga cccagaacac gaagcacgtg atgaagaccg cccagtacac cgggtgggag
     3601 tagcccgcga gcccaagtcc gtagcggagc aggaagagca ggcagacgat gccgaccgcc
     3661 agcaacgaga ccgccgcggc gagcagcgtg tagtaaccct tccccggatt acccagggtc
     3721 cgcgcgatgg cgcgcgtgac gaaatcatag gtcacgcggg aggcgggcgt gccggtgtcg
     3781 gccatcgctt acgcctcctc gcccgcgccg gcgcgcaccg cagcgagata cgtgacgccg
     3841 ggcttcgtat tcagtcgcgc cagcacgtgg tagccgcgcg gatcctcggc caactgggcg
     3901 acgcgcgagc ggggatcgtt caggtcgccg aaaacgatgg cctccgaggg acaggtctgc
     3961 gcgcaggccg gcacgatgtc gccgtcacgc accgtgcggt cctccatccg cgccgcgttc
     4021 tgggcgccgc ggatgcgctg gacgcagaag gtgcatttct ccatcacgcc cttctcgcgc
     4081 accgtcacgt cgggattgag ctgccagttc agcggcgcgg cgaacgcctc ccagtggccg
     4141 cccggctcgg catagttgta ccagttgaac acgcggacct tgtaggggca gttgttggag
     4201 caatagcgcg tcccgacgca gcggttgtag atctggccgt tcagcccgtc ggtcgtgtgg
     4261 taggcggcga acacggggca gaccggctcg cacggcgcgt tgccgcattg ctggcacagc
     4321 atcggcgagg tcaccgcgga gaccgacgcc ccgtcgaccg tggggcggaa gtaccgctcg
     4381 atccgcagcc aggccatctc gcggccgcgc accacgaggt cctcgcccac cgtggcgacg
     4441 ttgttctcgg cgtaacaggc cgtcacgcac gccgagcacc cggtgcattt gcccacgtcg
     4501 atcgccatcg cccagcgggg gtggtcgccc gcgtagttgc cgagcgacgc cttctcgtgc
     4561 tgcgcctcgg cccatccctc ctcggcggcc cgggcgtatt ccggcactcc ctccgcttcg
     4621 atcgcgtgct cgccggcgtg caggccgcgc gcccgctcga gcgacagcac cggtaccacg
     4681 tcctcaggca gctggcgccc ggtaccctcg gtggacgcaa gccggcggtg gtcggtggtc
     4741 ttggcgaccg tcaccgcgac gacatgcgtg cggccgccgt agtcattcgc tgcgacatcg
     4801 agcaggtcga acgccttgaa cgagcggttc tgggcatagc ggccgtaggc cgtgtgcccc
     4861 tgccccgtcg gcagcgccac gacgcccgga cgcaccgatg gcgtgatcca caccggtgcg
     4921 ttcacgccgc cggcggccga tttcacgagc agcacgtccc cgctggccag ctgccacttc
     4981 gccgcggtgt cggggtggac ctccacccaa ccgtgccagg tgagcttgga gaccggatcg
     5041 ggcagctcct gcagccacgg cttgttggcg ccgcggccgt cgtgcagcgc cggatgcggg
     5101 aagatcacgg ccacggcgtc gcccgtgaac gacggcgcgg cagcggccac cccggcggcg
     5161 gccgccgcga gccgcacgga gcgggcgggg gcgtcaccgt acacgccgcc gcgctgcagg
     5221 gcgtcggtcc accactggtc gaagcccggc ccacgcggcc ccgccagacg ctgatggagc
     5281 gcctgccatt tgccctgcag ggaggtcttg aaggagccgg gcttccccgc cgcgcgcagc
     5341 agcacatcgc ccgtctgcat cgtgccgtcg aacaccggca gcatcgccgg ctgctgtagc
     5401 gcgtacacgc ccgcgcgcgg aaggctgtcg ctccattgct cgagcggatg gtgatcgggg
     5461 aggacgagat cggcgccggc ggcggtctcg tcgagatacg acgaaaagct caccttgaag
     5521 ggcaccttcc cccacgcctg ggcgaacgcc ccgcccagcg catagaccgg gttggcgccg
     5581 tgcaccagca gcaccgcgac gtttcccgcg gccatatccg cggtgagctc ggacagctcg
     5641 cgatggctgc cggccgcgcc gctccccggc gccaggtctg ccccgaactt cacggtcttg
     5701 cctaccgctc cggcgacgta gttcaggatg ttgaccgccg ccacgatctc ggcgccgttg
     5761 ggatagtgag ccgccatgcc accggccacc gccaagcctg cgccggcggc gacgaactcg
     5821 cgcgccagcc gctcgatcgt ctccgcgtcg aggcccaccg cctgcgccac cgacgccggc
     5881 gcgggcagcg acagccgggc ggcgtcggcc ggaacggcgg ccagccgctg gcgcacgatc
     5941 acctgggcca tggcgagcgc caggagccct tcggtccccg gggccacggg cagccactcg
     6001 tccgcggcga gcgcggtctg cggcatgcgc ggacccacgt aagcgaactt cgccatgccg
     6061 gacgacgtgc cggactcgaa gctgtgggcg tgcgtgaagg cgttctggaa gccgactggc
     6121 gagagccagg tctccatgaa gtcggcgccg aaggagagga tgtaccccgc cgcggcgaag
     6181 tcgtaggccg gaacgctcgc gtcgccgaac gccagccggt tgccctcgcg cagcgcctcg
     6241 aacccgaacg gctcgcaggt gacgcgccgc ccgcccgcct ctttggtcca ttcgtccacc
     6301 agatccccga agctgcccga ctcgagaccg gtcacgaaga cgagcccccg gtcccgcgcc
     6361 tcggccacct tggaccgcag catcgtgatc gcatcatccc agctgaccgg ctcgaattga
     6421 ccggcggcgt tgcgccgcat cgggccggtg acgcgatcgg gattgtagag cccctgcagc
     6481 gccgcctgac cccgcgcgca cagccgcccg tggttcaccg gcgaggcggg gttcccctcg
     6541 atcttgaggg cgcgcccttc gcgcaccttc acctgaatgc cgcacccggc cgcgcattcg
     6601 cggcaggtgc tggcgtagta ggtcgcgatg ccgggagtct ggccttcggg cggcacgaca
     6661 tagggaatga gcttctcggt cggctcggga ccgatcccgc agcccgacag caccgcggcg
     6721 ccgccgcccg tcaccccgag gaccttcagg aaccgtcgcc ggttcatccc gtcgtgcata
     6781 tcgtctccga cgccccggct aatagtgaca caccgagcag tctcggctga gtttgcgttc
     6841 catgtgacac tggatgcacc agcccatctt cagcgtcttc acctgggaga tctgggtcgt
     6901 cccgttcacc gcgccgtggc aattggcgca ggcgtccggc gtgttgccca acaccttgat
     6961 gtgccgcatg tggggggagt gcacgaagcc gggaacccag tacacgcgga tccactccgg
     7021 gggtttccgg tcgctccaca gcttgcgcac tttggcgatc tcggcctgat gcgagggcgt
     7081 ggacccgccg atgatctgat ggcacccgat gcaggtgcgc agcgccggca tcccgggctc
     7141 gctcgagacc gtcacggtgt tgtggcaata gaggcagggg atgcgatcct gcccggcgtg
     7201 aatgtcgtga cggaagaaga tcggctgtcg ggggcccgcg agcgtcgcag tgtcggtgat
     7261 gacgggggct ttgtagcctg cagtgccggg tgccggaaca gcgccttgtg ccgccagagg
     7321 gacggctccc atgaccagga aaatcgacac cagaaatccg gtcgcggctc tgccccctct
     7381 ggtgatcacc aaagcgctgg cctcgtatgg caaaggctta gtacagcagt ggatgcgggc
     7441 gcggggaatg tagtgcggcc gccgaacgcc gcacaagtcc cgctcagtcg ggccgcgaca
     7501 acagatggtg cttgagctga atgacgccga cgaatccgtc gaccgtaccg aagtcgggga
     7561 agtccatcca gcaccagcgc acgccttcca ccagccggcc gtcgcgtacc gcctgcttca
     7621 cggaccgcgg caagccgtcg gccaggacca gctcctgctc ctcggcgtcg ttgagcacga
     7681 aggtgccgct cacgcccgat tccatgggat gcaggaatcc cagcttgcgg ccgccgacct
     7741 cgtacatcca cacccacttc cactccgtac ccatgtagac gatctgctcc gtcacgtggc
     7801 gcagcgcacg aatgttctcg cgcgcccgct tgaaccgccc ggcggcgagt ttggggagat
     7861 cacgcgccgg cgcgtcggac ttgggtcggg cccgcggact catgtagagg ttggtcgccc
     7921 agtatccatt cggtaggcgg gacatcagcg ctcacgggtt ggggatcagg gttgactgca
     7981 gggcacagga agttactggt gcggtcaaca cgtcgtcgta tgtctctttg tgtcaagcac
     8041 ttggaccggc gaggcgagac cgcgagccgc tcagggcagg accatcaggg atcgggtcac
     8101 cgccccgaag cgcgcagcat cgagcagccc acggcccgat tgctccggca ccgccatgcg
     8161 cccgtcgcga cccatggtca gctcgatcac gtcctggtcg ggcccgtacc accgcgcgct
     8221 cggctccagg tccgagggat acacgcaacg cggcagtgcc gcgacggcca agggcgcctg
     8281 ggacccgatg ccggattcgg gcatcgtccc ggcccacagc tccgccccgt acgactccgc
     8341 gatccggtag atgcggcaaa cttcggtgag tccgcccacc cggtgcactt tgatgttcca
     8401 cacgcgcggc cccgcgagct ccacgatctg acgcgcgtgg ctcgcgctct tgagggtctc
     8461 gtcgagacag atcggcgtgc ggagcgtttg ccccagttgg gcgtgtccca cgagctcttc
     8521 cggtgccagc ggctgctcga tgtacagcag gccggtctcg tccagcctcc gcaggtctcg
     8581 ctcatgcccg ggccattcgt aggcgccgtt ggcgtccacc gtcagcggca ggtccacgcc
     8641 cgccatcgca tcccgtgcgg cgcgcaccgc tccctcgtcc cagcccggca tcaccttgat
     8701 tttcacacgc cggtagccta gctcgagcgc ggcaccgacc cagcgcgcca gtgtcgcggg
     8761 gcggcgatcc tccggaatcc caagcgcgac gccgcagggg atggccggca tccgcgacgt
     8821 ctccagccgc tccccgagca gcgcggcgag ccccgtgccg cgccggtccg cctcgagatc
     8881 ccacgctgcc gtctcgattc ccgcccgggc gaacgggttg ccgcgcaccc ccacgcgcag
     8941 caccgcatcg atctccccgg ggtccgcgaa ttcccggccc acgactcgcg gcagcagcac
     9001 ccgggcgatg agatcgccgg cgccggccag cgtctcctcc gaatagaacg gcagctcgaa
     9061 cggcggcgac tctccgtagc ccgtgtgtcc ggtggcgtcg tgcagcgcga cgatgagcga
     9121 ccgtcgctcc cggatcgcgc cgcccgagat gatgaacggc tcgaccaggg gcagcgtgag
     9181 ctcgaacagc tcagcccttg agacgagcat gcggcagatc gaggaagtgc agctcgccgc
     9241 cccgccggtg gatcgcgccc tgctgctcga ggcgccacag ctcggcgagg agaccggatt
     9301 gcgggtgaaa cacgagctgg tgcagcgcgc gctgggtgcg cgatccggac cgggccgagt
     9361 actcgtccag cgggagctgg aagaaggtgt gcag
//
LOCUS       DBNGZW010000323         6190 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1529984, whole genome shotgun sequence.
ACCESSION   DBNGZW010000323 DBNGZW010000000
VERSION     DBNGZW010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6190)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1529984"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(258..503)
                     /locus_tag="AC16JC_07690"
     CDS             complement(258..503)
                     /locus_tag="AC16JC_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013143.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HIR5164613.1"
                     /translation="MGIDVRRLDLVYLLAFALTVALGLRSLGVLLMGSLIIIPATTAQ
                     YVARSLGSMLSVTVVISVASSSSGRSALRCGTLRPVR"
     gene            complement(607..936)
                     /locus_tag="AC16JC_07695"
     CDS             complement(607..936)
                     /locus_tag="AC16JC_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011297346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR5164614.1"
                     /translation="MPTFIMLTRVSPEALRSPRGVEVLERVAMTAVRAECPQVEWLES
                     YALLGPYDYLDIFRAKDIETATKVATLVRIGGHAHTEVWPATEWTRYKEMVREMPDFG
                     AVISVTG"
     gene            complement(963..1295)
                     /locus_tag="AC16JC_07700"
     CDS             complement(963..1295)
                     /locus_tag="AC16JC_07700"
                     /inference="COORDINATES: protein motif:HMM:NF014534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043024 - ribosomal small subunit binding
                     [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HIR5164615.1"
                     /translation="MQLPLQVAARGVALGDETETLIRQYAEKLDHYYDRIMGIRVMVE
                     VPQRQMGEPIMHNVRIDMTVPGGEVVVKRQEHPELHTAIQRAFEAARRRLQDYARRQR
                     GEVKARAE"
     gene            complement(1306..2721)
                     /locus_tag="AC16JC_07705"
     CDS             complement(1306..2721)
                     /locus_tag="AC16JC_07705"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011555388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HIR5164616.1"
                     /translation="MPAVLQELAHEIPVTAQPGMRVPARVYADDELWEQIGGDRSLVQ
                     LKNVATLPGVTGAVYGMPDMHEGYGFPVGGVAAMRLSDGVISPGGVGYDINCGVRLLA
                     SGLRAERIRAKLPDLVHEISRSVPSGAGRGGRLSFSDAELDRVFVEGCRYLAERALAE
                     PDDLEVIEAGGCLPGADPARVSARARQRGQDQLGTLGSGNHFLEVQVVDEVFEPHAAE
                     ALGLAPGQVTVLVHTGSRGLGHQVCTDYVRLLDEAMPRYGIVLPDRELACAPFESPEG
                     KRYFGAMCAAANFAWSNRQVITQRLREAFRRFFGAAGALRIVYDVAHNMAKVEEHGGE
                     RLCVHRKGATRAFGPSHPETPERYRSLGQPVFIPGSMGTGSFVLVGTDEAMTLSFGSC
                     CHGAGRRMSRHAAKRTQPGHEVRRELEARGIVVACPSSGELAEEAPFAYKDVERVVNV
                     VHQAGLARKVVRLRPLGVIKG"
     gene            complement(2729..3139)
                     /locus_tag="AC16JC_07710"
     CDS             complement(2729..3139)
                     /locus_tag="AC16JC_07710"
                     /inference="COORDINATES: protein motif:HMM:NF014058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0072669 - tRNA-splicing ligase complex
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006388 - tRNA splicing, via
                     endonucleolytic cleavage and ligation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HIR5164617.1"
                     /translation="MKTSVELVEHPGEVRLRLRAARLGDLAGVAGRALAEVELGHPPG
                     PARGAWREITIHGRDRDAVLVHWLNELIYLAETDRWVGVEFAADRATETELRMRARGI
                     PVDETPSRVKAATFHGLRIAPVADGFEADLVLDV"
     gene            3261..3728
                     /locus_tag="AC16JC_07715"
     CDS             3261..3728
                     /locus_tag="AC16JC_07715"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5164618.1"
                     /translation="MNSHAATLPIYQVMTTTPVTVHPMTTVSELLALFDRYDFNAFPV
                     VAADGTLRGIVSKLDVLRLLHPDDQFRLPDPKVIGATRVVDIMRPGILTVEAEDPVVV
                     AADLMVATGRRSLPVVRRGSGWPALVGIVSRGDLLRGLRFELAEEVPARAVTA"
     gene            complement(3652..>6190)
                     /gene="mgtA"
                     /locus_tag="AC16JC_07720"
     CDS             complement(3652..>6190)
                     /gene="mgtA"
                     /locus_tag="AC16JC_07720"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015436610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015444 - P-type magnesium transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HIR5164619.1"
                     /translation="LTGSAAVQRLSVAGPNLLRIHHEPTFLPLLLSRFRNPLVILLVA
                     ASVIAALTGDVTSFVIIVLAVVLSVTLDTIQEHRAGQAAERLRQSVAMRTIAVRDGQP
                     TEVPAGDIVPGDVVLLSAGDLVPADGRVLKARDCYVNQALLTGEPYPAEKAAREEGEP
                     DTQMADARGALFMGTSVVSGTATMLVCHTGRQTALGEIGESLQTHPPPSAFELGTRGF
                     GLLILRLVVLMVLFVILVNMARHRPWLESFLFAVALAVGLTPELLPMVVSVTLSRGAL
                     RMAAKKVIVKRLAAIHDLGSMTVLCTDKTGTLTEAHIRLERHLDPLGRDSTRLLELAY
                     LNSHFQAGLKTPLDEAILRHTEVDARGWEKVDELPFDFERRRVSVLVAREGGPPLLLV
                     KGALEDIIRLSARYEADGPADLRALDDAARRAVTARFEALSREGFRVLGVAWKPAEAA
                     GHRIVSADETELVFAGFAAFEDPPKASAAVALSDLARRGVAVKVLTGDNEIVTQHLCR
                     ELGVPVAAVLTGAEIDHLGDHALEARVGTVTLFCRVTPPQKNRVILALKRRGQVVGYV
                     GDGINDAPSLRSADVGISVDGGVDVAKEAADLIMLEQDLGVLHDGVVEGRRTFGNIMK
                     YIMMGTSSNFGNMFSMAGASLVLPFLPMLPTQILLNNLLYDVSEVPIPMDEVDDEYVE
                     RPQHWDMRFIRNFMLAVGPVSSLFDFLTFFVMLRVFRAGEELFQTGWFIESMATQVLV
                     IFIIRTRGLPWRSRPSRLLTLTSLGVVAAAIALPLTPLGGRLGFVRPPLTFFLILAAM
                     VATYLLGVEAVKRWFYRRFAGDARRSPPAPALPPPARTAGRAAGRRG"
BASE COUNT         1040 a         2242 c         1964 g          944 t
ORIGIN      
        1 cacacggcac gtcggggcgc tggtccgacg cggatgaggc tcgcgatggc ctcaccactc
       61 caggcgaggt gatcactcgg atcgacggtt tcctctccgt tgccggtcgg gaagcctccc
      121 cgtacccacc tgaagaataa tctcgaactt acgcccggcc cctgaagccg gcgtgaatcc
      181 aacgtgcagg tttcccagtc aggcgcgtcg acgtaccgcc aaaccaagca gaaagaagcc
      241 ggcagcgctc gcgatgatca gcggaccggt cgcaaggtgc cacagcgaag cgccgagcgt
      301 cccgaggagc tggaagccac ggaaatgacg accgtcacgc tgagcatgct tcccaggctg
      361 cgggccacgt actgcgccgt cgtggctgga atgatgatga gcgaccccat gagcagtacg
      421 cccagggagc gcagcccgag cgccaccgtg agcgcgaaag cgagcagata cacgaggtcg
      481 agccgccgca catcgattcc caccgtgcgc gcgttccgcc ctgcggcacg acgaaactca
      541 caccggcgag ggcgggcgtg ccgccgatcc ggacgccgag gccttcgacc tcgagcgcgt
      601 tcggcgtcat cccgtcacgc tgatcaccgc cccgaaatcg ggcatctccc gcaccatctc
      661 cttgtagcgc gtccactcgg tggccggcca cacctcggtg tgcgcatggc cgccgatccg
      721 gaccagcgtc gcgaccttcg tcgccgtctc gatgtccttg gcgcggaaga tgtcgaggta
      781 gtcgtacggc ccgagcaacg cgtagctctc cagccactcc acctgcgggc actcggcccg
      841 cacggcggtc atcgcgacgc gctccagcac ctccactccc cgcggcgaac gcaacgcctc
      901 gggcgacact ctggtcagca tgatgaacgt cggcatggga tcctctctgt tccctatttt
      961 ctctattctg cccgcgcctt gacctcgccc cgctggcgtc gggcgtagtc ctgcaggcgg
     1021 cgccgggcag cttcgaatgc ccgctggatc gcagtgtgca gctccggatg ctcctggcgc
     1081 ttcaccacga cctccccgcc ggggaccgtc atgtcgatgc ggacgttgtg catgatcggc
     1141 tcgcccatct ggcgctgagg gacctccacc atgacgcgaa tgcccatgat gcgatcgtag
     1201 taatggtcga gcttctccgc gtactggcgg atcagcgtct ccgtctcgtc ccccaatgcg
     1261 acgccgcgag cggcgacctg caatggcagt tgcatggctt cctcctcagc ctttgatgac
     1321 gccgagcggg cgcaaccgca cgaccttccg cgccaggccc gcctgatgca caacgttcac
     1381 gacccgctcc acgtccttgt aggcgaacgg cgcctcctcg gcgagctcgc cactcgacgg
     1441 gcaggccacg acgatccccc gcgcctcgag ctcgcgccgc acctcgtgcc cgggctgcgt
     1501 ccgcttggcg gcgtgccgac tcatgcggcg ccccgcgccg tgacagcagc tcccgaagct
     1561 cagcgtcatc gcctcgtccg tcccgacgag cacgaaggag ccggtcccca tgctccccgg
     1621 gatgaacacc ggctgcccca gagagcgata gcgctccggc gtttcggggt gcgatggtcc
     1681 gaatgctcgc gtcgcgccct tgcgatgcac gcacagccgc tcgccgccgt gctcctccac
     1741 cttcgccata ttgtgggcga cgtcgtacac gatccgcaac gcgcccgcgg ctccaaagaa
     1801 gcgccggaac gcctcgcgca gccgctgggt gatcacctgc cggttcgacc acgcgaagtt
     1861 cgccgccgcg cacatcgcgc cgaaatagcg cttcccctcg ggcgactcga acggcgcaca
     1921 tgccagctcg cggtccggca gcacgatgcc gtagcgcggc atcgcctcgt ccaggaggcg
     1981 cacgtagtcg gtgcacacct gatgacccaa gccgcgggac ccggtgtgga ccagcaccgt
     2041 gacctgcccc ggcgcgagac ccagcgcctc cgccgcgtgc ggctcgaaga cctcgtccac
     2101 cacctgcact tcgagaaagt ggtttcctga cccgagggtc cccagctgat cctgaccgcg
     2161 ctgccgggcc cgggccgata ccctcgcagg gtcggcgccc ggcagacagc cacccgcctc
     2221 gatcacctcg aggtcgtccg gttccgccag ggcacgctcc gcgagatacc ggcatccctc
     2281 tacgaagacg cgatccagct cggcgtcgct gaaggacaat cgtccgcccc ggccggcccc
     2341 cgacggcacg ctgcgcgaga tctcgtgcac cagatcgggc agcttggcgc gaatgcgctc
     2401 cgcgcgcagt cccgatgcga gcagccggac gccgcaattg atgtcgtaac ccacgccacc
     2461 cggagagatg acgccgtcgg acagccgcat cgccgcgact cccccaaccg ggaagccgta
     2521 gccctcgtgc atgtcgggca tcccatatac ggcgccggtc acgcccggca gcgtggcgac
     2581 gttcttgagc tgcaccagcg agcgatcgcc gccgatctgc tcccagagct cgtcgtcagc
     2641 atacacgcgc gccgggacgc gcatccccgg ctgcgccgtc accgggatct cgtgagccag
     2701 ctcctgcagt actgccggca tggcatcctc agacatcgag aaccaagtcc gcttcgaatc
     2761 catcggccac gggggcgatc cgcaggccgt ggaacgtcgc ggccttcacc cgcgagggcg
     2821 tctcgtccac ggggatccct cgcgcccgca tccgcaattc cgtctccgtc gcccggtccg
     2881 ccgcgaactc tacccccacc cagcgatccg tctcggccag gtaaatcagc tcgttgagcc
     2941 agtgcaccag caccgcatcg cgatcgcggc cgtggatggt gatctcccgc cacgcacctc
     3001 gcgcaggtcc cggcggatgg cccaactcca cctccgcgag cgcccgaccc gcgacgccgg
     3061 cgagatcgcc cagcctggcg gctcggagac ggagccgtac ctcgcccggg tgctcgacga
     3121 gctcgaccga agtcttcatt tccacttgta tgctgccacg gcatcgatga aacggccttg
     3181 agcggacaat gtttcccgct tcacgaaggc ttcatcgcgg gacgtgcagc attcagcatc
     3241 cggctgcctc ggaggccccg atgaacagtc acgctgcaac gttgcccatc tatcaggtca
     3301 tgaccaccac cccggtcacg gtgcatccca tgacgaccgt gtcggagctg ctggcgctgt
     3361 tcgaccgcta cgacttcaac gcgttcccgg tggttgccgc ggacggtacg ctgcgcggca
     3421 tcgtgtcgaa gctcgacgtg ctccggctgc tgcatccgga cgaccagttc cgcctcccgg
     3481 acccgaaggt gatcggcgcg acgcgcgtcg tcgacatcat gcggccgggg atcctcaccg
     3541 tcgaagccga ggatccggtc gtcgtcgcgg ccgacctcat ggtggccacc gggcggcgca
     3601 gcctccccgt ggtgcggcgc ggcagcgggt ggcctgcgct ggtggggatc gtcagccgcg
     3661 gcgacctgct gcgcggcctg cggttcgagc tggcggagga agtgccggcg cgggcggtga
     3721 ccgcctagca tcgccggcga agcgacggta gaaccagcgc ttcacggcct cgaccccgag
     3781 aagatacgtc gcgaccatcg ccgcgaggat caggaaaaag gtgagcggcg gccgcacgaa
     3841 tccgagccgg ccgccgagcg gcgtcagcgg tagggcgatc gccgctgcca cgacgccgag
     3901 cgaggtcagc gtgagcagtc tgctgggacg gctgcgccac ggcaggccac gcgttctgat
     3961 gatgaagatc accaggacct gggtggccat cgactcgatg aaccaccctg tttgaaacag
     4021 ctcctcgccc gcccggaaca cgcgcagcat cacgaagaac gtcaggaaat cgaacagcga
     4081 gctgaccggt ccgacggcga gcatgaaatt ccggatgaac cgcatgtccc agtgctgcgg
     4141 gcgctcgacg tactcgtcgt ccacctcgtc catggggatc gggacttcgg atacatcgta
     4201 tagcaggttg ttcagcagga tttgcgtcgg gagcatcggc aggaacggca gcacgagcga
     4261 ggctcctgcc atgctgaaca tgttcccgaa attcgagctc gtccccatca tgatgtactt
     4321 catgatattg ccgaaggtgc ggcggccctc gacgaccccg tcgtgcagca cgcccaggtc
     4381 ctgctcgagc atgatgaggt ccgctgcctc cttggccaca tccacgcccc catccaccga
     4441 aatccccacg tccgcggagc gcagggacgg ggcatcgttg atcccgtcgc cgacgtaccc
     4501 gaccacctgg ccgcggcgct tgagcgcgag gatgacgcga ttcttctgcg gtggagtgac
     4561 gcgacagaac agcgtcaccg tgccgactcg cgcctcgagt gcgtgatcgc ccaggtggtc
     4621 gatctcggcg ccggtgagca ccgccgcgac ggggacgccg agctcccggc acaggtgctg
     4681 ggtgacgatc tcgttgtctc ccgtcagcac cttgacggcg acgccgcgac gcgccaggtc
     4741 gctgagcgcc acggcggcgc tggccttcgg cgggtcctcg aacgccgcga agcccgcgaa
     4801 cacgagctct gtttcatccg ccgagacaat ccgatggccg gccgcctccg ccggtttcca
     4861 ggcgaccccc aggacgcgaa agccctcacg actcagcgcc tcgaagcgcg cggtcacagc
     4921 ccgtcgagcc gcgtcatcca gcgcacggag gtccgctgga ccgtccgcct cgtaccgggc
     4981 cgacaagcgg atgatgtcct ccagggcgcc cttgaccagg agcagcggcg ggccgccttc
     5041 ccgggcgacc aggaccgaga cccggcgccg ctcgaaatcg aacggcagct cgtccacctt
     5101 ttcccatccg cgggcgtcga cctccgtgtg gcgcaggatc gcttcgtcga gcggcgtctt
     5161 gagcccggcc tggaaatggc tgttcaggta ggcgagctcg agcagccggg tgctgtcgcg
     5221 gcccaatgga tccaggtgcc gctccagacg gatgtgcgct tcggtcagcg tcccggtctt
     5281 gtcggtgcac aggacggtca tgctgccaag gtcgtgaatg gcggcgagcc gcttcacgat
     5341 gaccttcttc gcggccatcc ggagagcgcc ccgcgacagc gtgacggaga cgaccatcgg
     5401 caacaactcg ggcgtgagcc ccacggcgag cgcgacggcg aacaaaaatg attcgagcca
     5461 gggccggtgc cgagccatgt tcacgaggat cacgaacagc accatgagca ccacgagacg
     5521 cagaatcagg aggccgaaac cgcgtgtgcc gagctcgaat gccgacggtg gggggtgcgt
     5581 ctgcaggctc tcgccgatct ctcccagagc ggtctgccgt ccggtgtgac agaccagcat
     5641 ggtggcggtg ccgctcacca ccgacgtacc catgaacagg gcgccgcggg cgtcggccat
     5701 ctgcgtgtcc ggctctccct cctcccgggc ggccttttcc gcgggatatg gctcaccggt
     5761 cagcagcgcc tgattcacgt agcaatcgcg cgccttcagc acccgcccat ccgcgggtac
     5821 gaggtcgcca gcggacagga ggaccacgtc ccccggcacg atgtcgcctg cgggcacctc
     5881 tgtcggctga ccatcccgca cggcaatcgt ccgcatcgcc acggactgcc gcaatcgctc
     5941 agcggcttgc ccggcgcggt gctcctgtat ggtatcgagc gtgacgctca gcacgaccgc
     6001 caggacgatg atcacgaagc tggtcacgtc ccccgtgagg gcggcgatca cgctggccgc
     6061 gacgagcaga atcacgagcg ggttgcggaa ccgtgacaac aacaacggca ggaaggtcgg
     6121 ctcgtggtgg atgcgcagca ggttcgggcc cgcgacgctc agccgctgga ccgccgcaga
     6181 gccggtgagg
//
LOCUS       DBNGZW010000324         4109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_981503, whole genome shotgun sequence.
ACCESSION   DBNGZW010000324 DBNGZW010000000
VERSION     DBNGZW010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4109
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_981503"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="AC16JC_07725"
     CDS             complement(<1..940)
                     /locus_tag="AC16JC_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR5164620.1"
                     /translation="MFKLLSTSGDQSIELAPGRTLVVGRAVTSDVPIYDPTISRRHAE
                     ITLVAGGVRVKDLGSSNGTFLNGARITQADAAANDVVTFGKVAFKVAGITPPVRPSAP
                     APEVGTTGGSRAMPQGTIVRQLPVSASSGGVKAIALETGPAAVGASHLKVKAENETER
                     REKKLELLLEISKELSRQQELDQLLHKVVDVTFQIMNVDRVSILLLDGKTKELVPKVS
                     RSRTGDTNAAKLVPQSIARKAVEDRVAILSDNAAADDRFKGKSILIQSVRSAMCTPLL
                     GSDQQALGIIYVDNQTAIHSFTDEDLEFLIAFGNIVT"
     gene            complement(987..2480)
                     /locus_tag="AC16JC_07730"
     CDS             complement(987..2480)
                     /locus_tag="AC16JC_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012470593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HIR5164621.1"
                     /translation="MTDPRADFLTRAQRGGVVPLAREIVLDADTAVAAYAKIARPPFG
                     FLLESLTGGERWARYTFLGTEPREAWRYRGRSVERWTAESGWRPAGETDDPIDHIGAR
                     LRPLAPVPVPGLPPFSGGAVGYLGYDLVRAMERLPTPPPDSLDLPDALLMIADSLVVL
                     DNLFGRAIVVANVEIPTEALARDRVRLYEAAEARLDTLVERLTQPHPLAPLPWPERPP
                     TLPAPPHSRYSRADFERDVARILEHIRAGDTFQTVLSRRQEIPRAVDAFRVYRYLRAL
                     NPAPYLFYLALDDVALVGSSPEVLVRVAGREVTVRPIAGTCRRGTTPEEDEALARALR
                     EDGKETAEHRMLVDLGRNDVGRVAAFGSVRVSELMGVERYSHVQHLVSEVRGALRDGY
                     DALDAFKACFPAGTVTGAPKVRAMEIIDALEPERRGPYAGAVGYVGWGAATLDTAIAI
                     RCALVRPERVVVQAGAGIVADSDPAREFAETEAKAEAVLRAVACALA"
     gene            2765..2893
                     /locus_tag="AC16JC_07735"
     CDS             2765..2893
                     /locus_tag="AC16JC_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164622.1"
                     /translation="MRVGTMVDVSASSVSLRETTLGGKNVKSANTVWLLSECVIAQ"
     gene            3013..>4109
                     /locus_tag="AC16JC_07740"
     CDS             3013..>4109
                     /locus_tag="AC16JC_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164623.1"
                     /translation="MRLLSALVCAVMLPAVAQAQRWNTPEALALADRAIARRAGSAAD
                     TSLHDYRARAHGFLFFLGAFGEDLDQPPRLIKADQLELEVYWKAPGASKQRIIGWRDR
                     AELPTDIVYHQDHLGIVQNGFGRTIRLGEGDEVRDVPHPLAPGGPVLYDYALGDTVVV
                     DLPEREIRVVALRFRPQDFQAPRIVGTAYLDAGSADLVRLAFNFTAVAYLDPTLEDVS
                     VVLDNALYQTRWWLPRHQEIEIRRRATLLDLPARGIIRGRWDVDRYDFNTGLSPTWFA
                     GPEISAAPKTVRDTFPWSTSLADAVQDVAEPVRRNDLAAVRAQAAALAGRHALSGWRH
                     RGLAVRGLSDLLHANRVQGVVPGAGVVWRSGL"
BASE COUNT          588 a         1396 c         1503 g          622 t
ORIGIN      
        1 tcgtcacgat attcccgaac gcgatgagga attccaggtc ctcgtcggtg aaggagtgga
       61 tcgcggtctg gttatccacg tagatgatgc cgagggcctg ctggtcggag ccgagcagcg
      121 gggtgcacat cgcgctgcgc acgctctgga tgaggatcga cttgcccttg aaccggtcgt
      181 cggcggcggc gttgtcggaa agaatcgcga cgcggtcttc cacggctttc cgcgcgatcg
      241 actgcgggac gagcttggcg gcgttggtgt cgccggtgcg gctgcgcgag accttgggga
      301 ccagctcctt ggtcttgccg tcgagcagca ggatcgacac ccgatccacg ttcatgatct
      361 ggaacgtgac gtccaccacc ttgtgcagca gctgatccag ctcctgctgg cgcgagagct
      421 ccttggagat ctcgagcagc agctcgagct tcttctcgcg gcgctcggtc tcgttctccg
      481 ctttcacctt gagatgcgac gcgcccaccg cggcggggcc ggtttcgagg gcgatcgcct
      541 tcaccccgcc cgacgaggcg ctgacgggca gctggcgcac gatggttccc tggggcatgg
      601 cgcgcgagcc gccggtcgtg cccacttcgg gcgccggggc cgagggacgg acgggcggcg
      661 tgatccccgc gaccttgaag gcgaccttgc cgaacgtgac gacatcgttg gcggcggcgt
      721 cggcctgggt gatgcgggcg ccgttcagaa aggtgccgtt cgaggagccg aggtccttca
      781 cccgcacgcc gcccgcgacg agggtgatct cggcgtggcg gcgggagatg gtgggatcgt
      841 agatgggcac gtcgctggtg acggcccggc cgacgaccag cgtacggccc ggcgcaagct
      901 cgatcgactg gtcgccggag gtgctcagca gcttgaacac ggtgcgaaaa tataccccga
      961 tcgtaccggg acggaatcgc gtcgcctcac gcgagcgcgc acgcgaccgc ccgcaggacg
     1021 gcctccgcct tcgcctcggt ctcggcgaac tcgcgcgccg gatcggagtc cgcgacgatg
     1081 ccggccccgg cctggaccac cacgcgctcg ggacggacca gggcgcagcg gatcgcgatc
     1141 gccgtgtcga gggtggccgc gccccagccc acgtatccca cggcgccggc atagggcccg
     1201 cgccgctccg gctccaaggc gtcgatgatc tccatcgccc gtactttcgg cgccccggtc
     1261 acggtccccg cgggaaaaca cgccttgaag gcgtcgagtg cgtcgtagcc gtcccgcagc
     1321 gcaccgcgca cctcgctcac caggtgttgg acatgggagt agcgctccac ccccatcaac
     1381 tccgagacgc gcacgctgcc gaaggcggcc acgcgaccca cgtcgttgcg accaaggtcc
     1441 acgagcatgc ggtgctccgc ggtttccttg ccgtcctcgc gaagcgcccg cgccagcgcc
     1501 tcgtcctcct caggggtggt cccgcgccgg cacgttcccg cgatcgggcg caccgtgacc
     1561 tcccggccgg cgacgcgcac cagtacttcg ggggaactgc ccacgagggc aacgtcgtcg
     1621 agggccaggt agaacaggta cggggcggga ttcagggcgc gcagatagcg gtacacccgg
     1681 aacgcgtcca ccgcgcgggg gatctcctgg cggcgcgaca gcaccgtctg gaacgtgtcg
     1741 ccggcgcgga tatgctcgag tatgcgcgcc acgtcgcgct cgaagtccgc ccgggagtag
     1801 cggctgtggg gtggtgccgg cagcgtcggc ggacgttcgg gccacgggag cggcgccagc
     1861 ggatgcggct gcgtcaatcg ctcgaccagc gtgtcgaggc gcgcttccgc cgcttcgtac
     1921 agtcggacgc gatcgcgggc cagggcttcg gtgggaatct cgacgttcgc cacgacgatg
     1981 gcgcggccga agaggttgtc gagcaccacg aggctgtccg cgatcatcag aagcgcgtcg
     2041 gggagatcga gcgaatcggg cggcggagtg gggagccgct ccatcgcccg taccagatcg
     2101 tagcccaggt agcccacggc gccgccgctg aacggcggca gtccggggac cggcaccggg
     2161 gcgagggggc gcagcctggc cccgatgtgg tcgatggggt cgtccgtctc gccggccggc
     2221 cgccagccgc tctcggccgt ccagcgctcc accgagcgtc cgcgataccg ccaggcctcg
     2281 cgcggctcgg tcccgaggaa cgtatagcgc gcccagcgct cgccacccgt cagggactcc
     2341 agcaggaacc cgaacggcgg cctggcgatt ttggcgtagg ccgccacggc cgtgtcggcg
     2401 tcgagcacga tctcgcgcgc cagcgggacc acgccgcccc gctgcgcgcg ggtcaggaaa
     2461 tcggcccggg gatcggtcac ttcggggcgg cgagcaggga ggtgccgggg ccttcgatgg
     2521 cgctgctggc ggtcgacggc cccgtgaggg tgctgttgcg gcccgggaac cagtggggag
     2581 aggtgcgctc gtgccacccc ccagcgcctg gatgaagtcg gacgttgaag cgtccttcgg
     2641 actctgggtg tatacggacg ggtccccgta ggccccgaac gagacgccga cgacgggctc
     2701 gttcgactgg ctgaagccca ggcgggcggg ggcgatggtg ccttcgtagc tgccgggcga
     2761 cgccgtgagg gtcgggacga tggtggacgt gtcggcgagc agcgtgtcgt tgcgcgagac
     2821 aacactgggg gggaagaacg tgaagtcggc gaacaccgtg tggctgctgt cggagtgcgt
     2881 gatcgcacaa tgatcgagca gagtgttctt gaagctgcac agcgtcccgc cgggcgtatc
     2941 tcggccgatg gcgaggagcg cgaagatggt cgcgcctgcc gcgcccacgt gccggcgggt
     3001 aggttggcgc ccgtgcgatt gctgtccgcg ctggtctgcg ccgtgatgct ccctgccgtg
     3061 gcgcaggcgc agcggtggaa cacccccgag gcccttgccc tcgccgatcg cgccatcgcg
     3121 cggcgggcgg gctccgccgc cgacacctcg ctgcacgatt accgggctcg ggcgcatgga
     3181 ttcctgttct tcctcggcgc gttcggagag gacctcgacc agcccccgcg tctgatcaag
     3241 gccgaccagc tcgagttgga agtgtactgg aaggccccgg gcgcgagcaa gcagcgcatc
     3301 atcgggtggc gtgatcgcgc cgagctgccg acggacatcg tctaccatca ggatcacctc
     3361 ggcatcgtgc agaacgggtt cggccgcacc atccgcctgg gcgagggcga cgaggtgcgc
     3421 gacgtgccgc atccgctggc gcccggcggt ccggtgctgt acgactacgc cctgggcgac
     3481 acggtcgtcg tcgacctgcc ggagcgcgag atccgtgtgg tcgccctgcg cttccggccg
     3541 caggacttcc aggcaccacg catcgtcggc accgcctacc tggacgccgg cagcgccgac
     3601 ctcgtccgcc tggcgttcaa tttcaccgcg gtcgcgtacc tggatcccac gctcgaagac
     3661 gtgagcgttg tgctcgacaa cgccctgtac cagacccgct ggtggctgcc gcggcatcag
     3721 gagatcgaga tccggcggcg cgctacgctg ctggatctgc cggcgcgcgg catcatccgc
     3781 gggcgctggg acgtggaccg ctacgacttc aataccggac tgagcccgac atggttcgcc
     3841 gggccggaga tcagcgcggc tcccaagacg gtgcgtgaca ccttcccctg gtccacgtcg
     3901 ctcgccgacg ccgtccagga tgtcgccgag ccggtgcggc gcaacgatct ggcggcggtg
     3961 cgggcgcagg ccgcggcgct cgcggggcgg cacgcgctgt cgggctggcg gcatcgcggg
     4021 ctcgcggtgc gcggcctgag cgatctgctg cacgccaacc gggtgcaggg ggtggtgccg
     4081 ggggccggcg tcgtctggcg atccggact
//
LOCUS       DBNGZW010000325         3391 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1872333, whole genome shotgun sequence.
ACCESSION   DBNGZW010000325 DBNGZW010000000
VERSION     DBNGZW010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1872333"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="AC16JC_07745"
     CDS             complement(<1..181)
                     /locus_tag="AC16JC_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164624.1"
                     /translation="MRILTIAAAVLTALSARAAAQGPRFEITIPAALRAAPLTGRVYV
                     FVSTKADEEPRLGDHG"
     gene            complement(263..1027)
                     /locus_tag="AC16JC_07750"
     CDS             complement(263..1027)
                     /locus_tag="AC16JC_07750"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019986361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR5164625.1"
                     /translation="MAARPYILSEITWKAVSDTRYEVAVLPWGATEAHNTHLPYGTDN
                     VETEGIATRAARLAWDAGARVLVLPLVPFGVQTGQLDIPFCLNLNPSTQALVLRDLAR
                     SLDGQGIHKLVVLNGHGGNDFRQMIREVQVGLRLFLCTVSWYVVVDPREYFSDLGDHA
                     GEAETSAMLYLAPELVRPLAEAGPGAARPWKLRALREGWAWAPRQWTKVTGDTGVGNP
                     QRATPEKGARYVQDITRQIADFFIELATADPDDLYG"
     gene            1091..1558
                     /locus_tag="AC16JC_07755"
     CDS             1091..1558
                     /locus_tag="AC16JC_07755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164626.1"
                     /translation="MRSSYVLLLALVGPLAAQTPRDTGFAALQQRGKQVMGVDQYTSL
                     HRFDDLPDGGRIVLTRSALDTAGVRTIRAHLEGLVRAFEAGDFGHTMAVHQHELPGTA
                     IMRERRAAISYRVDTLPGGGAVRITTRDTTAVRAIHRFLAAQRGEHHAGGAMP"
     gene            complement(1569..2933)
                     /locus_tag="AC16JC_07760"
     CDS             complement(1569..2933)
                     /locus_tag="AC16JC_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013786241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5164627.1"
                     /translation="MTFPRFLAVCLLGLPAAGVAQQSPAGPAVPPGSDTRLYRIVDSV
                     SARRIERDIRTLVGFHTRNTLSDTVSRSRGIGAARRWIKGQFDSISAACGGCLDVFYV
                     RQLVDSGRRIPKPVWVVDVVAVLRGQSDTGRYLAITGHYDSRATNVLDDTVMAPGADD
                     DASGTAAVIEAARVLSRYRFAASIAFGALAGEEQGLLGGRLMAQEAKARGWRVEADLN
                     NDIVGGARGIDGRSDNTVVRVFSESPPTGTTAEALPRLRVFGGEVDGPSRQIARYVHR
                     IAEDYLFGFRVMMVYRQDRFGRGGDHIAFNAEGFPAVRFTEPHENYNRQHQTVRVENG
                     VFYGDVPDSVDFTFTARVTALNAVTLAALAWAPPPPDSATVAGAVSATTTLRWKAVAA
                     PDLAGYKVYWRATTSPTWDHWRWVGNTTSATLDLVVDDAFFGVAAVNASGDESPVSYP
                     SPGR"
     gene            complement(2960..>3391)
                     /locus_tag="AC16JC_07765"
     CDS             complement(2960..>3391)
                     /locus_tag="AC16JC_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020600506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164628.1"
                     /translation="FDDLTRSGRDSLLTEQGAGAWPNQFRSARFIPAVEYLEASRVRR
                     LLQQDMQALLRDIDVYVSPAFSGANLLVTNLTGHPCVAVPDGFLDNDPASITFCGRMY
                     GEAEALEVARAYQAATPWDEMHPVTFGGSAPVVVAPRDTTR"
BASE COUNT          518 a         1151 c         1236 g          486 t
ORIGIN      
        1 cgccgtggtc accgaggcgg ggctcctcgt cggccttggt ggagacgaag acgtacacgc
       61 gcccggtcag cggggccgcg cgcagggcgg ccggaatcgt gatctcgaag cgcgggccct
      121 gggcggccgc gcgggcggag agagcagtga ggacggcggc ggcgatggtc aggatgcgca
      181 cgggcactcc agttagagaa gggcgacgtc caagtgtcgc ggacgggacg gggcgccgca
      241 agtgcgggcc cgcggcgtcc gctcagccgt agagatcgtc gggatcggcc gtggcgagct
      301 cgatgaagaa gtcggcgatc tgccgcgtga tgtcctgcac gtagcgcgcg cccttctcgg
      361 gagtcgcacg ctgcgggttc ccgacgcccg tgtcgccggt caccttggtc cattgccgcg
      421 gcgcccaggc ccagccttcg cgcagggcgc ggagcttcca cggccgggca gcacccgggc
      481 ccgcctcggc gagcggtcgc accagctcag gggcgagata gagcatcgcg ctcgtctcgg
      541 cctcgcccgc gtgatcgccg agatcggaga aatactcgcg cggatcgacg accacgtacc
      601 agctcaccgt gcacaggaag aggcggaggc ccacctggac ttcccggatc atctggcgga
      661 agtcgttgcc gccgtggccg ttcagcacca ccagcttgtg gatcccctgg ccgtcgagcg
      721 aacgggccag gtcgcgcagc acgagcgcct gggtgctggg gttgaggttg aggcagaagg
      781 gaatgtcgag ctgtccggtc tgcacaccga acggcaccag cggcaggacc agcacccgcg
      841 cgccggcgtc ccacgcgagc cgcgcggcgc gggtcgcgat cccctcggtt tcgacgttgt
      901 cggtgccgta ggggagatgg gtgttgtgcg cctcggtcgc gccccagggc aacacggcga
      961 cctcgtaccg cgtatcagag acggccttcc aggtgatctc gctgaggatg taggggcggg
     1021 cggccatgga tggactcggt ccagttgggg gtgatcaacg atagtcggcc ggacaaggag
     1081 actcgacaca atgcgatcgt cgtatgtact gctgctcgcg ctggtcggac cgctcgccgc
     1141 ccagacgccc agggacacgg ggttcgccgc cctgcagcag cgggggaagc aggtcatggg
     1201 cgtcgaccaa tacaccagcc tgcaccgctt cgacgatctg cccgacggcg ggcgcatcgt
     1261 cctcacccgc tcggccctcg atacggccgg cgtgcggacg atccgcgcgc acctcgaggg
     1321 attggtgcgg gcgttcgaag cgggcgactt cggccacacc atggcggtgc accagcacga
     1381 gctgcccggg acggcgatca tgcgcgagcg ccgcgccgcg atcagctacc gggtggacac
     1441 cctcccgggc ggcggggcgg tccgcatcac gactcgcgac acgacggccg tgcgagcgat
     1501 tcatcggttc ctggcggcgc agcgcggcga gcaccatgcc ggcggcgcga tgccttagcc
     1561 gggcttccct accgtcccgg cgacgggtag ctgaccgggc tctcgtcacc cgaggcgttc
     1621 accgccgcca ccccgaagaa cgcgtcgtcc accaccagat cgagcgtcgc cgaggtcgtg
     1681 ttgccgaccc agcgccagtg atcccacgtg ggtgacgtcg tggcccgcca gtacaccttg
     1741 tagccggcga gatcgggggc cgcgaccgct ttccagcgca gcgtcgtggt cgcgctgacc
     1801 gcgccggcga cggtcgcgct gtcgggcggc ggcggcgccc aggcgagcgc ggcgagcgtc
     1861 accgcgttca gcgccgtgac ccgggctgtg aaggtgaagt ccaccgagtc ggggacgtcc
     1921 ccgtagaaga cgccgttctc cacgcgcacc gtctggtgct gccggttgta gttctcgtgc
     1981 ggctccgtaa agcgcacggc ggggaacccc tccgcgttga aggcgatgtg atcgccgcca
     2041 cggccgaagc ggtcctggcg gtacaccatc atgacccgga aaccgaagag atagtcctcg
     2101 gcgatgcggt ggacgtaccg cgcgatctgg cgcgacgggc cgtccacctc gccgccgaac
     2161 acgcgcagcc gcggcaaggc ttcggccgtc gttccggtgg gcgggctctc cgagaagacg
     2221 cgcacgacgg tattatcgct gcgcccatcg ataccgcggg cgccgccgac gatgtcgttg
     2281 ttcagatcgg cctcgacccg ccagccgcgc gccttcgcct cctgcgccat gagccggccg
     2341 cccagcagac cctgctcctc acccgcgagc gcgccgaagg cgatcgaggc ggcgaagcgg
     2401 tagcgcgaca gcacccgcgc ggcctcgatc acggccgcgg tgccgctggc gtcgtcgtcg
     2461 gctcccggcg ccatgactgt gtcgtccagc acgttggtgg cgcgcgagtc gtagtgtccc
     2521 gtgatggcga ggtagcgtcc ggtgtcgctc tggccgcgca gcacggcgac gacgtccacc
     2581 acccagaccg gtttggggat ccgccggccg gaatccacca gctgccgcac gtagaacacg
     2641 tcgaggcagc cgccgcaggc ggccgagatg ctgtcgaact gacctttgat ccagcgtcgc
     2701 gcagcgccga tgccgcgact gcgggagacc gtgtcggaca aggtgttgcg cgtgtgaaag
     2761 ccgacgaggg tgcggatgtc gcgctcaatg cgccgcgccg acaccgaatc gacgatccgg
     2821 tagagacggg tgtcgctccc tggcggaacg gcggggccgg ccggggactg ctgggccacg
     2881 ccggcggccg gcaggccgag gagacagact gcgaggaaac gggggaacgt cacgatcgat
     2941 cctccgggtt ggaatgccgc tatcgggtcg tgtcgcgcgg cgccacgacg acgggtgcgc
     3001 tgccgccgaa cgtcacgggg tgcatttcgt cccacggggt ggctgcctgg taggctcgcg
     3061 caacctcgag tgcctcggct tccccgtaca tccggccaca gaaggtgatg ctggccggat
     3121 cgttgtcgag aaacccgtcc ggcaccgcga cgcacgggtg gccggtcagg ttggtcacca
     3181 gcaggttcgc gccggagaac gccggcgaga cgtacacgtc gatgtctctg agcaacgcct
     3241 gcatgtcctg ttgcagcagg cggcgcaccc ggctcgcctc gaggtactcc acggcgggaa
     3301 tgaagcgcgc tgagcggaac tgattgggcc atgcgccggc gccttgctcg gtgagcaggc
     3361 tgtcgcgccc ggagcgcgtg agatcgtcga a
//
LOCUS       DBNGZW010000326         3165 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1461462, whole genome shotgun sequence.
ACCESSION   DBNGZW010000326 DBNGZW010000000
VERSION     DBNGZW010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3165)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3165)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3165
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1461462"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="AC16JC_07770"
     CDS             complement(<1..1047)
                     /locus_tag="AC16JC_07770"
                     /inference="COORDINATES: protein motif:HMM:NF024603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HIR5164629.1"
                     /translation="MLLKRRRAAVPLALALAAACANTGEPPGGPPDEKPPVIIGVYPE
                     SGAVVPNPRGAAVIQFDGVIDEQSGGGGVGATAGLASRILLSPTAGPVDVSWHRSSIH
                     VKPKEGWKPGRVYQLQVLPGIADLRRNVLKERHTIVFSTGPAIPHGSLSGAAVAWVEQ
                     RALVGGLIRAQLLPDTIAYLALVDSSGGFSLDNLPPGRYAVFAIADQNGNRVRDRREA
                     YDSALVTLDSSATAMLWTFLHDTAGPRLRQVDPVDSVTARVTFTQSLDPAQHPESLGV
                     RVLALPDSTPVRVRGIFTQALYDSLAAQERAARDTTKHAPPAPRDTTHEREVDTVAPP
                     PRVPGGGAPAQDTSG"
     gene            complement(1038..>3165)
                     /locus_tag="AC16JC_07775"
     CDS             complement(1038..>3165)
                     /locus_tag="AC16JC_07775"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF004349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HIR5164630.1"
                     /translation="EEAEFRDTSGTLYYINYPLVEGSGYVTVATTRPETLLGDTAVVV
                     HPEDPRGAPFIGKHVRLPITGIDIPVLADEAVEKEFGTGFVKVTPAHDANDFEIGLRH
                     GLGMPLVIRENGTMGDDGGDRKGDTEPRVPADLTGLDRFEARKQIVRQLEALGLLVKT
                     EPHQHAVRRCYRCDSIVEPRLSDQWFVRMQPLAEPVLAAYRRGEFRIVPERWHATFLN
                     WMENIRDWNISRQLWWGHRIPVFTCTRCRHQWADREDPAACPECGGPVEQDPDVLDTW
                     FSSWLWPFATLGWPEETPDLRRFYPGHTLVTGSEILFFWVARMLMAGYHFLGQKPFST
                     VYLTGTVRDTKHRRMSKSLGNGIDPLDVVKLFGADALRWTMIAGGSLGADVILDPADL
                     ETTFAPGRNFANKLWNIARFILAQLPADVPRLEDQDPRTLTLADRWILSRAQRAVTET
                     TAALEQFRLDEAARRCFEFAWGELADWYVETVKPRLAADDPSARSAQAVLAHCWDTAL
                     RLLHPVVPFITEELWQKLPGRRPGELLILAPWPAADASWTNPDADAQFGAVQQAITAI
                     RNIRAEYRVPPKVRLAAAIRPAGRSARATLEAEHDTILRLAQLESLATDGEALEGAHA
                     VLTDGSEVVVALAGAIDVQQECRRLSDELQRLDKQLDGLAAKLANESFVARAPAEVVA
                     REREKQEAWRTQRDVLAAKLGALGCS"
BASE COUNT          436 a         1095 c         1163 g          471 t
ORIGIN      
        1 ccccgaggta tcctgggccg gggcgccgcc gcccggcact cggggaggcg gggcgaccgt
       61 atcgacttcg cgctcgtgcg tggtgtcgcg cggggcgggc ggggcgtgtt tcgtcgtgtc
      121 ccgggcggcg cgttcctgcg ccgcgaggga gtcatagagc gcctgcgtga agatcccgcg
      181 cacgcggacc ggggtggaat cgggcaacgc cagcacgcgg acgcccaatg actcgggatg
      241 ctgcgcggga tcgagcgact gcgtgaacgt gaccctcgcc gtgaccgaat ccaccgggtc
      301 cacctggcgc agtctcggcc cggcggtgtc gtgcaggaac gtccacagca tcgccgtggc
      361 gctcgaatcg agggtcacca gcgcggagtc gtacgcctcg cggcggtccc gcacgcggtt
      421 gccgttctgg tcggcgatcg cgaagacggc gtaccggccg ggcggcaggt tgtcgaggga
      481 gaagccgccg ctggaatcga cgagggcgag ataggcgatg gtgtccggga gcagctgggc
      541 ccggatcagc cctcccacga gcgcccgctg ctccacccat gccacggccg cgccgctcag
      601 gctgccatgg gggatcgccg gccccgtgct gaagacgatc gtgtggcgtt ctttcaggac
      661 gttgcgtcgc aggtccgcga tcccgggaag gacctggagc tgatagacgc gccccggctt
      721 ccatccttcc ttgggcttga cgtggatcga ggagcggtgc caggacacgt cgaccggccc
      781 cgcggtggga gagagcagga tgcgcgacgc caggccggcc gtagcgccca ccccgccgcc
      841 gccgctctgc tcgtcgatga cgccgtcgaa ctggatcacc gcggcgccgc gtggattggg
      901 cacgaccgcc cccgactccg ggtacacacc gataatcacc ggcggtttct cgtcgggcgg
      961 cccgccgggg ggttcgcccg tgttggcaca agccgccgcc agggcgaggg cgaggggtac
     1021 cgcggcgcgg cgccgcttca ggagcatccc agggcgccca gcttcgcggc cagcacatcg
     1081 cgctgggtgc gccacgcctc ctgcttctcc cgctcccggg ccacgacctc ggcgggtgcg
     1141 cgcgccacga agctctcgtt ggcgagcttc gcggcgagac cgtcgagctg tttgtcgaga
     1201 cgctgcagct cgtcggagag ccgccggcat tcctgctgga cgtcgatcgc gcccgccagc
     1261 gcgaccacga cctcgctgcc gtcggtgagc acggcgtgcg cgccctcgag cgcttcgccg
     1321 tcggtggcca gcgactcgag ctgggcgagg cgcaggatgg tgtcgtgctc ggcttcgagc
     1381 gtcgcccggg cggaccgtcc cgccggccgg atcgcggccg cgaggcgcac cttgggcggc
     1441 acacggtatt ccgcccggat gttgcggatc gcggtgatcg cctgttgcac cgcgccgaac
     1501 tgcgcgtccg cgtccggatt ggtccaggac gcgtcggccg cgggccaggg cgccaggatg
     1561 agcagctcgc ccgggcggcg ccccgggagc ttctgccaca gctcttcggt gatgaacggc
     1621 acgacggggt gcagcagccg cagcgccgtg tcccagcagt gcgccagcac ggcctgcgcg
     1681 ctccgcgcac tcgggtcgtc ggccgccagg cgcggcttca ccgtttccac gtaccagtcg
     1741 gccagctcgc cccaggcgaa ctcgaaacag cgccgcgccg cctcgtcgag gcggaactgc
     1801 tccagggccg cggtcgtctc ggtgacggcg cgctgtgccc gcgacaggat ccagcggtcc
     1861 gcgagggtga gcgtgcgggg gtcctggtct tcgagccgcg gcacgtcggc gggaagctgg
     1921 gccaggatga agcgggcgat gttccagagc ttgttggcga agttgcggcc cggggcgaac
     1981 gtcgtctcga gatcggccgg gtcgaggatc acgtcggcgc cgagcgagcc gccggcgatc
     2041 atcgtccagc gcagcgcatc ggctccgaac agcttcacca cgtcgagcgg atcgatcccg
     2101 ttgccgagcg atttcgacat gcggcggtgc ttggtgtcgc gcaccgtgcc ggtgaggtag
     2161 acggtgctga acggcttctg cccgaggaag tggtagccgg ccatcagcat gcgcgccacc
     2221 cagaagaaca gaatctcgga gccggtgacg agcgtgtgtc ccgggtagaa gcgccgcagg
     2281 tcgggcgttt cttccggcca gcccagcgtg gcgaacggcc agagccagga ggagaaccaa
     2341 gtgtcgagca cgtccgggtc ctgctccacg gggccgccgc attcggggca ggcggccggg
     2401 tcctcccggt cggcccactg gtgccggcag cgggtgcagg tgaacaccgg gatgcgatgg
     2461 ccccaccaca gctgccgcga gatgttccag tcgcggatgt tttccatcca gttcaggaac
     2521 gtcgcgtgcc agcgctccgg cacgatgcgg aattcgcccc ggcggtaggc ggccagcacc
     2581 ggctcggcga gcggctgcat ccgcacgaac cactggtcgg acagccgcgg ctcgacgatc
     2641 gagtcgcagc ggtagcagcg ccgcacggcg tgctggtgcg gttcggtctt gacgagcaga
     2701 cccagcgcct cgagctgccg cacgatctgc ttgcgggcct cgaagcgatc gagccccgtc
     2761 agatcggcgg gcacgcgggg ctccgtgtcg cccttgcgat cgccgccgtc gtcgcccatg
     2821 gtcccgttct cgcggatgac gagcggcatg ccgagcccgt ggcgcagccc gatctcgaag
     2881 tcgttggcat cgtgcgccgg ggtcaccttg acgaatccgg tgccgaactc cttctccacc
     2941 gcctcgtcgg cgagcaccgg gatgtcgatg cccgtgatcg gcagccgcac gtgcttgccg
     3001 atgaaggggg cgccgcgggg atcctcggga tgcaccacca cggccgtgtc gcccagcagc
     3061 gtctcgggac gcgtcgtggc caccgtgacg tagccgctcc cctccaccag gggatagttg
     3121 atgtagtaga gggtgccgct cgtgtcgcgg aactccgcct cctcg
//
LOCUS       DBNGZW010000327         3097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1664562, whole genome shotgun sequence.
ACCESSION   DBNGZW010000327 DBNGZW010000000
VERSION     DBNGZW010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1664562"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..166
                     /locus_tag="AC16JC_07780"
                     /pseudo
     CDS             74..166
                     /locus_tag="AC16JC_07780"
                     /inference="COORDINATES: protein motif:HMM:NF021520.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
     gene            163..1461
                     /locus_tag="AC16JC_07785"
     CDS             163..1461
                     /locus_tag="AC16JC_07785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164631.1"
                     /translation="MSAPAIPRPTAPHPGGVPLGSLDQTPPFALPGEHFAAGLVWLAL
                     GAAGLVMVAPLLAQGGFLAPRVIAVTHCFTLGWITTSIFGALYQLFPVALGVPARSVR
                     VGHATFWVLQAGVALLVAGTWWWRPPLIAAGWVVLFLAVGGLAWNVLPQRRRAPRGRA
                     IGLYISAAHMALGFAMAVAAARIGAELGWWRIDRLGLLAAHAHLAVVGFATLTAVGVG
                     SRLLPMFLLSHGHVEWPLRWIGPSVGAGLLLFATGELLSSRALTVSGALAMATGIALY
                     LALVAGYFAHRVRRALDPGLAHVAAAFLFLAFATVLGLLVFLLPGFRPRLIASYAVAG
                     IIGWLSLLVAGIYQKIVPFLTWLHRFAARVGEPGVPRIADLTVPAVGWTMLVLFAGSL
                     LLLLAGIAAGAGVLARTGAAGFAAGTALLVGQHARLALRR"
     gene            1458..1649
                     /locus_tag="AC16JC_07790"
     CDS             1458..1649
                     /locus_tag="AC16JC_07790"
                     /inference="COORDINATES: protein motif:HMM:NF013999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HIR5164632.1"
                     /translation="MTTKPEVEALQDVLVTMTLTTPGCPLGDAVTRGVERVVRELPWV
                     RSVRVAIVWDPPWSTAMIR"
     gene            1663..2070
                     /locus_tag="AC16JC_07795"
     CDS             1663..2070
                     /locus_tag="AC16JC_07795"
                     /inference="COORDINATES: protein motif:HMM:NF021520.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2249 domain-containing protein"
                     /protein_id="HIR5164633.1"
                     /translation="MSVTLDVRPALARGEEPFTMIMEAAAGVPDAGALELTAPFEPVP
                     LYAVLSRQGFGHVTAALPAGDFQVRFVRTGITPGATVAAVHERYPDTAATFAAHGIDL
                     CGGGGNTLEFVSKAHGVELARLLAELQQAVVKP"
     gene            2114..>3097
                     /locus_tag="AC16JC_07800"
     CDS             2114..>3097
                     /locus_tag="AC16JC_07800"
                     /inference="COORDINATES: protein motif:HMM:NF012424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HIR5164634.1"
                     /translation="MSEFVFGTNNAAFVQVMYEQYLRDPASVGEEWRVLFDNGRLAEL
                     PIIPTNRAEVLAALGSGEQGAVGVGAPSATAPSSPLPAGLTPITGPAARLVQNMAESL
                     GVPTATSFRDVPVAVLDTRRKALNAALAGAGLKVSFTHLIGYAIVQAVKQFPVMVRAF
                     HDLDGRPHRFDPDGIHLGLAVDVEKKDGSRTLVVPVIKHAEGMDFAAFHATYETLVEK
                     ARSNRLLPDDFAGAAITLTNPGTIGTVASVPRLMKGQGSIIATGAIRDSIGARIMTIT
                     STYDHRVIQGAESGMFLRKLEALLQGEDGFYDAVTASLGVSGGGGRGVVLSA"
BASE COUNT          382 a         1096 c         1087 g          532 t
ORIGIN      
        1 accgctgacc tgccacgcgc attacatcga aaccgtggag cttgcctgaa caaggtccga
       61 ccgcacgtac gacggtctcg cccccggcga gcgattcgtc ctgatcaacg accacgaccc
      121 ggagccgctt tacgaagagc ggcgcatcgc gatcggtcgc acgtgagcgc gccggccatt
      181 ccgcgcccca cggcgccaca tccgggcggt gtcccgctcg gctcgctcga ccagacgccg
      241 ccctttgcgc tgcccggcga gcacttcgcg gccggactcg tgtggctcgc gctgggcgct
      301 gccggtctcg tcatggtcgc gccgctgctc gcacagggcg gttttctggc gccgcgggtc
      361 atcgccgtca cgcactgctt cacgctgggc tggatcacca cgtcgatctt cggcgcgctg
      421 taccagttgt ttccggtggc gctgggcgtg ccggcgcgca gcgtccgcgt cggccacgcc
      481 accttctggg tgttgcaggc gggggtggcc ctgctcgtcg ccggcacctg gtggtggcgc
      541 cctcctctga ttgccgccgg ctgggtcgtg ctgttcctgg cggtcggcgg tctcgcctgg
      601 aacgtgctgc cgcagcgccg ccgggcgccg cgcggccgcg cgatcgggct ctacatctcc
      661 gccgcccaca tggcgctggg attcgcgatg gccgtggcgg cggcgcgcat cggcgccgag
      721 ctgggctggt ggcggatcga ccggctcggg ctgctggcgg cgcacgccca cctggccgtg
      781 gtcgggttcg cgacgctgac ggcggtgggc gtggggagcc ggctgctgcc gatgtttctc
      841 ctctcgcacg gacatgtcga gtggcccttg cgctggatcg gtccgtcggt cggcgcgggc
      901 ctcctcctgt tcgccaccgg cgagctgctc tcgagccgcg cgctcaccgt gtccggtgcg
      961 ctcgccatgg cgacggggat tgccctctat ctggcgctcg tcgccggcta cttcgcccat
     1021 cgcgtgcggc gcgcgctcga tcccgggctg gcgcatgtgg ccgcggcctt cctctttctg
     1081 gccttcgcta cggtgctggg gctgctcgta ttcctcctcc cgggttttcg accgcgcctc
     1141 atcgccagct acgccgtggc cgggatcatc gggtggctgt cgctcctcgt cgccgggatc
     1201 taccagaaga tcgtcccgtt tctcacctgg ctgcatcgct tcgccgcacg ggtcggagag
     1261 ccgggggtgc cacgcatcgc cgatctgacc gtgccggcgg tgggctggac gatgctcgtg
     1321 ctgttcgcgg ggagtctcct cctgttgctg gccgggatcg cggcaggcgc cggtgttctc
     1381 gcccggaccg gcgccgcggg attcgccgcc gggacggcgt tgctcgtcgg ccagcacgcg
     1441 cgactcgcgc tgcgccgatg acgacgaagc ccgaggtgga ggcgctccag gacgtgctcg
     1501 tgaccatgac gctcaccact ccggggtgcc cgctcggtga cgccgtcacg cggggcgtcg
     1561 agcgcgtggt gcgggagctg ccctgggtcc gttccgtccg cgtcgcgatc gtttgggatc
     1621 cgccctggag cacggcgatg atccgctgaa gaggagcagg caatgtcggt cacgctcgat
     1681 gtacggcccg cgctcgcccg gggcgaggag cccttcacga tgatcatgga agccgcggcc
     1741 ggcgttcctg acgccggagc gctggagttg acggcgccgt tcgagccggt gccgctctac
     1801 gcggtgctct cgcggcaggg gttcggccac gtcacggcgg cgctgcccgc gggagatttt
     1861 caggtccgtt tcgtccgcac cggcatcact ccgggcgcca ccgtcgcagc cgtccacgaa
     1921 cgctatccgg acacagccgc gaccttcgcc gcccacggca tcgacctgtg cggcggcggc
     1981 ggcaataccc tggagttcgt gtccaaggcc cacggcgtgg agctcgcccg gctgctcgcc
     2041 gagttgcagc aggcagtcgt caagccctga cccggcaatg ccgagcgctt gcaggccctc
     2101 ttagattgct ggggtgagcg agttcgtctt cggcaccaac aacgccgcct tcgtgcaggt
     2161 gatgtacgag cagtacctgc gcgatccagc ctccgtcggc gaagaatggc gcgtgctctt
     2221 cgacaacggc aggctggcgg agctgccgat tatcccgacg aaccgggccg aggttcttgc
     2281 ggctttgggg agcggggagc agggagcggt cggagttggt gcaccgtcgg cgaccgctcc
     2341 ctcctcccca ctccccgcgg gcctcacgcc catcacgggt cccgcggccc gcctcgtcca
     2401 gaacatggcc gagtcgctcg gcgtcccgac ggcgacctcc ttccgcgatg tccccgtggc
     2461 ggtgctcgac acgcggcgca aggcgctgaa cgccgcgctc gcgggcgccg gtctcaaggt
     2521 gtcgttcacc cacctgatcg ggtacgcgat cgtgcaggcg gtgaagcagt tcccggtcat
     2581 ggtccgcgcc ttccacgacc tggacggcag gccgcaccgc ttcgaccccg acggcatcca
     2641 cctcggcctc gccgtggacg tagagaagaa ggacggcagc cgcacgctcg tcgtgccggt
     2701 gatcaagcac gccgagggta tggacttcgc cgctttccac gccacctacg aaacgctggt
     2761 cgagaaggcg cgcagcaacc gactcctccc cgacgacttc gccggcgccg ccatcaccct
     2821 gaccaacccc ggcacgatcg gcacagtggc ctcggtcccg cggctgatga agggccaggg
     2881 ctcgatcatc gcgaccggcg cgatccgcga ttcgatcggc gcccggatca tgacgatcac
     2941 gagcacctac gaccaccggg taatccaggg cgcggagtcg gggatgtttc tccgcaagct
     3001 ggaggcgttg ctgcaggggg aggacgggtt ctacgacgcg gtaacggcga gcctgggggt
     3061 atcggggggt ggggggcggg gagtggtact ttccgct
//
LOCUS       DBNGZW010000328         4480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1324380, whole genome shotgun sequence.
ACCESSION   DBNGZW010000328 DBNGZW010000000
VERSION     DBNGZW010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4480
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1324380"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1159)
                     /locus_tag="AC16JC_07805"
     CDS             complement(<1..1159)
                     /locus_tag="AC16JC_07805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5164635.1"
                     /translation="MTDRIETLLDEQRSFTPPPAFRIQAHVRDPEVYERARTDREGYW
                     AEWARQLDWIRPWDRVLDWKPPHAQWFVGGKLNASANCLDRHVHAGRGDRTALIWEGE
                     PGEVRRLTYADLLDQVERCANVLKDLGVTRGDRVAIYLPMVPEVAVAMLACARIGAPH
                     TVVFGGFSAESLRDRINDATAKVLVTADGGYRRGNLVPLKQNADEALADTPSIRHVVV
                     LKRTGQPIAMQAGRDQWWEDLMGRAAAHCEPEAMDAEDPLYILYTSGTTGKPKGILHT
                     TGGYLTHVYATTKWVFDLKDSDVFWCTADVGWVTGHSYVVYGPLALGVTEVMYEGAPD
                     HPAKDRFWAICERHGVTVFYTAPTAIRAFMKWGTALPATHDLSHLRLLGTVG"
     gene            1498..1869
                     /locus_tag="AC16JC_07810"
     CDS             1498..1869
                     /locus_tag="AC16JC_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5164636.1"
                     /translation="MAAVGTKRKVLFIEDEAALQASYARFFSARYDMAFARTGVEAMQ
                     QLETFNPEVLVLDLRLPDTDGIALLQRIRQKRATIPVVVTTAYVSMEPLMHVLDLGHS
                     AYLVKPFDLNELAARIDAVGA"
     gene            1853..2533
                     /gene="ccmA"
                     /locus_tag="AC16JC_07815"
     CDS             1853..2533
                     /gene="ccmA"
                     /locus_tag="AC16JC_07815"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HIR5164637.1"
                     /translation="MLSARDLSHAYGSLRALDRITFKLDRGQTLAIFGPNGAGKTTLL
                     KVLAGLIHPRHGTVQLAGGRRAIGWIGHQSQLYGPLSVVENLLFWASLYDVPKATRRA
                     RAGELIGRLGLEGQAHQPARTLSRGQAQRAAIARALIHDPSVLLLDEPFTGLDRVAAE
                     ELRRLLGELAAAGRATVLVTHNVEEGTELASDIAFMRAGRFVAFGPRAGRDSASLAAA
                     YREAVGGG"
     gene            2526..3206
                     /locus_tag="AC16JC_07820"
     CDS             2526..3206
                     /locus_tag="AC16JC_07820"
                     /inference="COORDINATES: protein motif:HMM:NF015344.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA];
                     GO_process: GO:1903607 - cytochrome c biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:1904334 - heme import across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HIR5164638.1"
                     /translation="MADLVRHAWLVARKDLYLEFRSRTAITSALVFTALVLVVFEHGR
                     DPTAVATIDLAPSILWVTFSFAGMLTLNRAFNAELENQALDGLLLAPISRTSLFWGKL
                     VANLAFVAVVQITGLLLFTLFFNVPVWRVWAPLVAVMILATVGFIAVGTLFSAMVVRT
                     RFAELMLPVLLLPFLLPPLIAAVEVTAKLLGGRPLSEVAGWFRLLAAFDVTFVTLATL
                     LFPATVTE"
     gene            3203..3859
                     /gene="ccsA"
                     /locus_tag="AC16JC_07825"
     CDS             3203..3859
                     /gene="ccsA"
                     /locus_tag="AC16JC_07825"
                     /inference="COORDINATES: protein motif:HMM:NF013725.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HIR5164639.1"
                     /translation="MTLVRSGRWITGIALLLVAGVYVRALAYTPVEIHQGLAQKIYYV
                     HVPSAWSAMLAMGMVGLASILFLFLKDPRYDRFASASAEVGTAFSLVMLTTGPLWARP
                     VWNTWWTWDARLTLTLMLFFLFVGYQVLRGAVTDPGLRARYAAVVGICGMVLLPFIHL
                     SVYLFRTLHPMPIVLKPSKPSLPGSMLVTLLLSVGVFTLLYVGFVMQRYAIGERTDDA
                     "
     gene            3849..3974
                     /locus_tag="AC16JC_07830"
     CDS             3849..3974
                     /locus_tag="AC16JC_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164640.1"
                     /translation="MTPDQIPQTGGYMVAAYIVTAVILLAYTIVLWRRDSAKTKS"
     gene            complement(3978..>4480)
                     /locus_tag="AC16JC_07835"
     CDS             complement(3978..>4480)
                     /locus_tag="AC16JC_07835"
                     /inference="COORDINATES: protein motif:HMM:NF012455.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HIR5164641.1"
                     /translation="ISGGHLNPAVSLGVFVAGRIDGRTLGRYVLAQLVGGIAGAALLK
                     LLFASTAVRAVTAGAPQLSLNIGFGQGIAIEAAFTFFLVSAVFGTAISSEAPKIGGFG
                     IGLAIFVSALVVGGLTGAALNPARAFGPALVAWSFHSQAVYWIGPLAGGALAGWVWRV
                     LLLPKQ"
BASE COUNT          629 a         1530 c         1552 g          769 t
ORIGIN      
        1 cacccaccgt gccgagcagc cgcaggtgtg acaggtcgtg tgtggcgggg agagccgtgc
       61 cccacttcat gaatgcccgg atcgccgtgg gcgcggtgta gaacacggtg acgccgtggc
      121 gctcgcagat cgcccagaag cggtccttcg ccggatggtc cggtgctccc tcgtacatca
      181 cctcggtgac gccgagcgcg agcggtccgt acaccacgta ggagtgaccc gtcacccagc
      241 ccacgtcggc ggtgcaccag aagacgtcgg agtccttgag gtcgaacacc cacttcgtcg
      301 tcgcgtagac gtgcgtcagg tagccgcccg tcgtgtgcag gatgcccttg ggtttccccg
      361 tggtgcccga cgtgtacagg atgtagagcg ggtcctcggc gtccatcgct tccggctcgc
      421 aatgggcggc ggcccgaccc atcaggtcct cccaccattg atcccggccg gcctgcatcg
      481 cgatcggttg gccggtgcgc ttcagcacca cgacgtgccg gatgctgggc gtgtcggcga
      541 gcgcctcgtc ggcgttctgc ttgaggggga cgaggttgcc gcggcggtac ccgccgtcgg
      601 cggtgaccag caccttggcg gtcgcgtcgt tgatccggtc ccgcagcgac tcggcggaga
      661 agccgccgaa cacgacggtg tgcggcgcgc cgatgcgggc gcaggcgagc atggcgaccg
      721 cgacctcggg gaccatcggc aggtagatcg cgacgcggtc gccgcgggtc acccccaggt
      781 ccttcaggac gttggcgcag cgctccacct ggtccagcag gtcggcgtag gtgaggcggc
      841 ggacctcgcc gggctcgcct tcccagatca gcgccgtacg gtcgccccgg ccggcgtgga
      901 cgtggcggtc gaggcagttg gccgacgcat tcagcttgcc gccgacgaac cactgggcat
      961 gaggcggctt ccaatcgagc acccggtccc agggacgaat ccagtcgagt tgcctggccc
     1021 attcggccca gtacccctcg cgatcggtcc gcgcccgctc atagacctcc gggtctcgga
     1081 cgtgcgcctg gatgcggaat gcgggtggcg gcgtgaagct gcgctgttcg tcgagcagag
     1141 tctcgatgcg atcggtcatg ggtcgtggac ggtagaagtg agccgtaagg tatcgccggg
     1201 tcggtccggg gggtcaagcg gtttccagca tgcgccgggc catgcgcccc gccactcgcc
     1261 cggcgcccgg acgcgtcggg tccgcggatc gcaaccgctt gctgccgcgg cggttgccgg
     1321 aacgcctagg gccccatcgc catggctccg cagaccccgg tggaacggta tgcggttccg
     1381 agtccggggg cgtcgtgttc gtgccatgat gtccgcacac ccgtcgcagg cagtggttac
     1441 gcgtctcccg gccgcgcgcg tagacgcctt tcacgcggtg ggtatattgg ccaggtcatg
     1501 gccgccgtcg gcaccaaacg caaagtgctg ttcatcgagg acgaagccgc cctgcaggcg
     1561 tcgtacgccc ggtttttcag cgcgcgctac gatatggcgt tcgcgcggac cggcgtggag
     1621 gcgatgcagc agctcgagac cttcaatccc gaggtgctgg tgctcgatct gcggctgccg
     1681 gacacggacg gcatcgccct gctgcagcgg attcgccaga agcgcgccac catcccggtg
     1741 gtggtgacca ccgcctatgt cagcatggag ccgttgatgc acgtgctcga cctcggtcac
     1801 agcgcctacc tcgtgaagcc cttcgacctg aacgagctcg ccgcccgcat cgatgctgtc
     1861 ggcgcgtgac ctctcgcacg cgtacggctc gctgcgcgcc ctggatcgca tcaccttcaa
     1921 actggatcgc ggtcagacgc ttgcgatctt cggccccaac ggagccggca agaccaccct
     1981 gctgaaagtg ctcgcggggc tgatccaccc gcggcacggc acggtgcagc tcgcaggcgg
     2041 ccgccgcgcc atcggctgga tcggccacca gtcgcagctg tacgggccgc tgtcggtcgt
     2101 ggaaaacctg ctgttctggg cttcgctcta cgacgtgccc aaggcaacgc ggcgcgcccg
     2161 cgccggcgaa ttgatcgggc gcctcgggct cgaaggccag gcgcatcagc cggcgcgcac
     2221 cctgtcacgg gggcaggcgc agcgcgcggc catcgcccgg gcgctgatcc acgacccctc
     2281 ggtgctgctg ctggacgagc cgttcacggg actcgaccgt gtggcggcgg aggagctgcg
     2341 ccgcctgctg ggggaactgg cggccgcggg tcgcgccacg gtgctcgtca cccacaacgt
     2401 cgaggaaggc accgagctgg cgagcgacat cgcgttcatg cgggcggggc gcttcgtcgc
     2461 cttcggcccg cgcgccggtc gcgactcggc gtcgctggcg gcggcctacc gggaggcggt
     2521 cggcggtggc tgatctcgtg cggcacgcct ggctggtggc gcgcaaggac ctgtacctcg
     2581 agttccgctc gcggacggcg atcacgtcgg cgctcgtgtt caccgcgctg gtgctggtcg
     2641 tgttcgagca cggccgcgat ccgaccgccg tcgcgacgat cgatctcgcc ccgagcattt
     2701 tgtgggtgac gttcagcttc gccgggatgc tcacgctgaa ccgggcgttc aatgcggagc
     2761 tcgagaacca ggcgctcgac gggctgctgc tcgcgcccat cagccgcacc agcctgttct
     2821 ggggcaagct ggtcgccaac ctcgcgttcg tggcggtggt gcagatcacc gggttgctcc
     2881 tgttcacgtt gttcttcaac gtgccggtct ggcgcgtgtg ggcgccgctc gtcgcggtga
     2941 tgatcctggc caccgtgggg ttcatcgccg tcggcacgct gttcagcgcc atggtggtgc
     3001 gcacccggtt tgcggagctg atgctgccgg tgctgctgtt gccctttctg ctgccgccgc
     3061 tcatcgcggc ggtggaggtg acggccaagc tgttgggcgg ccgtcccttg agcgaggtcg
     3121 cggggtggtt taggttgctc gcggctttcg acgtgacgtt cgtgacgctc gcgacgcttc
     3181 tcttcccggc gacggtgacg gaatgactct ggtccgaagc ggacggtgga tcaccggcat
     3241 cgcgctcctc ctggtggcgg gggtgtacgt ccgagcgctg gcgtacaccc cggtggagat
     3301 ccaccagggg ctggcgcaga agatctacta cgtacacgtg ccatcggcgt ggtccgccat
     3361 gctggccatg ggcatggtgg ggctcgccag catcctgttc ctgttcctca aggacccgcg
     3421 ctacgaccgg ttcgcgtccg cctcggcgga agtcggcacg gcgttctccc tcgtcatgct
     3481 caccaccggc ccgctctggg cccgccccgt ctggaatacc tggtggacgt gggatgcgcg
     3541 actcacgctg accctgatgt tgttcttcct gttcgtcggc taccaggtgc tgcgcggagc
     3601 ggtgaccgat cccggcctgc gggcgcggta cgcggcggtg gtggggatct gcggcatggt
     3661 gctgctcccg ttcattcatc tcagcgtcta cctgttccgt accctccacc ccatgccgat
     3721 cgtcctgaag ccgagcaagc cgtccctccc cggatcgatg ctcgtgacgc tgttgctgtc
     3781 ggtcggcgtc ttcaccctgc tctacgtggg gttcgtgatg cagcgctacg ccatcggaga
     3841 gcgtaccgat gacgcctgac cagatcccac agaccggcgg gtacatggtc gccgcgtaca
     3901 tcgtgacggc cgtgattctc ctggcctaca cgatcgtgtt gtggcgcagg gactcggcga
     3961 agacgaaatc gtagtcccta ctgcttcggc agcagcagca ctctccagac ccaccccgcc
     4021 agcgcccctc ccgccagcgg accgatccag tacaccgcct gggagtggaa cgaccacgcg
     4081 accagcgccg gcccgaaggc acgcgccgga ttgagggcgg cgccggtgag tccacccacg
     4141 acgagcgccg atacgaaaat ggcgagtccg attccgaacc cgccgatctt gggcgcctca
     4201 ctcgagatcg cggtgccgaa cacggcggac acgaggaaga aggtgaacgc ggcttcgatg
     4261 gcgatcccct gtccgaatcc gatgttgagc gagagctggg gcgcgccggc cgtcaccgcc
     4321 cggaccgccg tgctcgcgaa caacagcttc aggagcgccg cgccggcgat cccgccgacg
     4381 agctgggcca gcacgtagcg gccgagggtg cggccgtcga tccgccccgc cacgaacacg
     4441 cccaggctca ccgccgggtt gaggtggccg cccgagatgc
//
LOCUS       DBNGZW010000329         3026 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1461744, whole genome shotgun sequence.
ACCESSION   DBNGZW010000329 DBNGZW010000000
VERSION     DBNGZW010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1461744"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..1029
                     /gene="queG"
                     /locus_tag="AC16JC_07840"
     CDS             112..1029
                     /gene="queG"
                     /locus_tag="AC16JC_07840"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR5164642.1"
                     /translation="MAALTRRARSLGFDAVGVAPLEPNPHARELDHWLEAGYAGTMTY
                     LHRQAAKRKDPRRIMPEARVAVVTLTNYFHDPATPQVTGKVAQYAWSRDYHEVLGDRL
                     DRLAAVIRELCPGARTRCYVDAGPVPERELAQRAGLGWIGKNTMLINPEIGSFTFLGT
                     VLTDAPLPPDPPFEADRCGTCRACLDACPTGAFAAPHVLDARRCISYLTIEHRGAFTD
                     EQRAWVGEWLFGCDVCQDVCPWNVRFARPTHDVDLATRDLPDPAEFSVIDAATFDFRY
                     ADTAFSRPGHAGMRRNAAAIRPAGARDRA"
     gene            1026..1289
                     /locus_tag="AC16JC_07845"
     CDS             1026..1289
                     /locus_tag="AC16JC_07845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164643.1"
                     /translation="MTASRIAEIQRQLTEGLARIDPHHRLAGRPTSFRVIDGQTLEIT
                     FRDVAGIAEAEVLGVKRMLGTECYCTVSPQTAEQITVRFVVPL"
     gene            complement(1286..2092)
                     /locus_tag="AC16JC_07850"
     CDS             complement(1286..2092)
                     /locus_tag="AC16JC_07850"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR5164644.1"
                     /translation="MKARIGWLVERLTAGLGRWEREVLQTPEVPVPIAGLSPDLAGYR
                     IALVTDLHNGPAVPRWWLARAVERVTSLAPDLIALGGDFVSHARSDLDGLESLLAGLR
                     APDGVVAVLGNHDHWVGPELVADVVRRAGTELLLNRHRLIRRGRSDLAVAGVDDLVHG
                     AVRVTDALEGVPAATPVVLLSHNPDLVEYLPPGLRIDLMLSGHTHNGQVHWPLIGPLT
                     VPSQFGRRYLHGLHHVRDTWLYVSAGVGSGAIPIRWGNPTELPVLRLVPA"
     gene            complement(2089..>3026)
                     /locus_tag="AC16JC_07855"
     CDS             complement(2089..>3026)
                     /locus_tag="AC16JC_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5164645.1"
                     /translation="DPATFPQAEPRFAVPRAPDDVLSITWVGHSTFLLQLGGLNILTD
                     PIWSDRASPIPGVGPKRLLAPGIPFDMLPPIDAVLQSHDHYDHLDDLTVCRLEARDPG
                     ARWFVPLGLGTFLRRRGVHEITELDWWQEASLGPLSIGCTPAQHFSGRVPWNRNRTLW
                     CGWSVASTSRRLLFAGDTGYHPEFAQVAQRFGPFDLALLPIGAYEPGWFMQPVHMNPE
                     EAVAAARDLGAPRLVPMHWGTFKLTDEPLDEPPQRALASWCAAGLPVERFWQLAHGET
                     RELPAGEARWLGPSSIPGGGGRRARSRARRISSGG"
BASE COUNT          481 a         1095 c         1046 g          404 t
ORIGIN      
        1 accgcctacg gcgacgaagc agcggtccca ccggtcgaag acgtcgtgcg gttcgcgcgc
       61 tggcggcggg agacgatgcg gctctgatga ccaggacgta tggatagcag cgtggcggcg
      121 ctgacacgcc gggcgcggtc gctgggattc gacgccgtcg gcgtcgcgcc cctcgagccc
      181 aacccgcacg cgcgggagct cgaccactgg ctcgaggccg gatacgccgg cacgatgacc
      241 tacctccacc gtcaggcggc gaagcggaag gacccacggc gcatcatgcc tgaggctcgc
      301 gtcgccgtgg tcacgttgac gaactatttc cacgatccgg caaccccgca ggtgacgggc
      361 aaggtcgccc agtacgcctg gtcgcgtgat taccacgagg tgctgggcga ccgcctcgac
      421 cggctcgcgg ccgtgattcg cgagctgtgc cccggcgcgc gcacccgctg ttacgtcgat
      481 gcgggaccgg ttcccgaacg tgagctcgcg cagcgggcgg ggctcggctg gatcggcaag
      541 aacacgatgc tgatcaatcc ggaaatcggg tcgttcacgt ttctcgggac cgtgctgacg
      601 gacgcgccgc tgccgcccga tccgccgttc gaagccgatc gctgcggcac gtgccgcgcc
      661 tgcctcgacg cctgcccgac cggcgcattc gccgcccccc atgtgctcga cgcccggcgc
      721 tgcatctcgt acctgaccat cgagcatcgt ggagcgttca cggacgagca acgggcgtgg
      781 gtcggcgagt ggctgttcgg ctgcgacgtg tgtcaggacg tatgcccctg gaacgtgcgc
      841 ttcgcgcgcc cgacccatga cgtcgatctc gcgacacggg atctccccga cccggcggaa
      901 ttctcggtga tcgacgcggc tacgttcgac ttccgctacg ccgacacggc cttctcccga
      961 ccgggccacg ccggcatgcg ccgcaacgcc gcggccatcc gtcccgccgg cgcgcgtgac
     1021 cgcgcatgac cgcgagccgc atcgccgaga ttcagcggca gctcaccgaa gggctggccc
     1081 gcatcgatcc gcaccaccgg ctcgccggcc ggcccacgag ttttcgggtg atcgacggac
     1141 agacgctcga gatcacgttc cgcgacgtgg cggggatcgc cgaagcggaa gtgctcggag
     1201 tcaaacgcat gctcggcacc gagtgctatt gcaccgtctc gccgcagacc gccgagcaaa
     1261 tcaccgtgcg gttcgtcgtc ccgctctagg ccggtaccag gcgcagcacg ggaagctcgg
     1321 tcggattgcc ccagcggatc ggaatcgccc cggacccgac gcccgccgag acatagagcc
     1381 acgtatcgcg cacgtggtgg aggccgtgca gatagcggcg cccgaattgc gacggaacgg
     1441 tgagcggccc gatgagcggc caatgcacct gaccgttgtg cgtgtgcccc gacagcatga
     1501 ggtcgatgcg cagacccggc gggaggtact ccaccaggtc ggggttgtgt gacaggagca
     1561 cgacgggagt cgctgcgggc acgccttcga gcgcgtcggt cacccggacc gcgccgtgca
     1621 cgaggtcgtc caccccggcg accgccaggt cgctccggcc ccggcggatc aggcgatgcc
     1681 ggttcaacag cagctctgtc cccgcgcgcc gcacgacatc ggccaccagc tccggcccga
     1741 cccagtgatc gtggttgccg agcaccgcca ccacgccgtc gggcgcacgc agtcccgcaa
     1801 gcaaggactc gaggccgtcg agatcgctgc gcgcgtgact cacgaagtcg ccgcccagcg
     1861 cgatcaagtc gggggccagc gacgtcacgc gctcgaccgc ccgcgcgagc caccaccgcg
     1921 gcaccgcggg gccgttgtgc agatcggtca ccagcgcgat gcggtagccc gcaagatccg
     1981 gcgagagccc ggcgatcggc accggcactt ccggcgtctg cagcacctcg cgctcccagc
     2041 ggcccagccc agccgtcagc cgttcgacca gccacccgat ccgcgccttc acccgccgct
     2101 cgaaatacgg cgggcgcgcg accgcgctcg gcggcctccg ccgccgggga tagaagatgg
     2161 gcccaaccac cgggcctcgc cggccgggag ctcacgcgtt tcgccgtgcg ccagctgcca
     2221 gaagcgctcc accggcagcc cggcggcgca ccagctcgcc agcgcccgct ggggcggctc
     2281 gtccaggggc tcgtcggtga gcttgaaggt gccccagtgc atcggcacga gccgcggtgc
     2341 ccccaggtca cgcgccgcgg cgacggcttc ctcggggttc atatggaccg gctgcatgaa
     2401 ccagccgggc tcgtaggcgc cgatcggcag cagggccaga tcgaacgggc cgaatcgctg
     2461 cgccacctgg gcgaactccg ggtggtagcc cgtatcgccc gcgaacagca gccgccggct
     2521 cgtcgacgcc acgctccagc cgcaccacag agtccggttc cggttccagg gcacgcggcc
     2581 gctgaagtgt tgggccggtg tgcatccaat ggaaagcggg ccgagcgacg cctcctgcca
     2641 ccaatcgagc tcggtgatct cgtgcacccc gcgccgccgg aggaacgtgc cgaggcccag
     2701 cggcacgaac caccgcgctc ccggatcccg cgcctcgaga cggcagacgg tgagatcgtc
     2761 caggtggtcg tagtggtcgt gggactgcag cacggcgtcg atcggcggca gcatgtcgaa
     2821 agggatgccg ggggccagga gacgcttcgg gccgactccg gggatgggcg acgcgcggtc
     2881 actccagatc gggtcggtga gaatgttcaa tccacccagc tgcagcagaa acgtcgagtg
     2941 gccgacccag gtgatggaca ggacgtcgtc cggggcgcgc ggcacggcga agcgcggctc
     3001 cgcctgcgga aacgtggcgg gatccg
//
LOCUS       DBNGZW010000330         2379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1461855, whole genome shotgun sequence.
ACCESSION   DBNGZW010000330 DBNGZW010000000
VERSION     DBNGZW010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1461855"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="AC16JC_07860"
     CDS             <1..786
                     /locus_tag="AC16JC_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011937483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HIR5164646.1"
                     /translation="LSKVLPYDHMKVQEYINSSWYEYSKGDEAGLHPYEGETKAKYTG
                     PPTPWTYLQDQQKYTWMKAPRYDGQPMEVGPLARMLVAYASGHKEVQEMVGATLKGLN
                     VGPAALFSTLGRTGARGIETVLLARRLSVWYDRLVANIKNGDTRTFDDSKWDPARWPA
                     KAQGYGYLDAPRGALGHWVQIEDQKIARYQCVVPSTWNCSPRDGKGVPGPYEAALMDH
                     HPLVDPARPIEILRTIHSFDPCMACGVHVLDATGREVTSVRVQ"
     gene            837..1562
                     /gene="cybH"
                     /locus_tag="AC16JC_07865"
     CDS             837..1562
                     /gene="cybH"
                     /locus_tag="AC16JC_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HIR5164647.1"
                     /translation="MRGISLAHKVPPPSGDYHWVYLWGWPIRAMHWLAVACIVVLVVT
                     GFYIGRPYFVTSGEASSHYLMGWVRFLHFASAGVLVATAIVRTYWLFVGNRFERWRAL
                     FPFSPRDWGRLWKQVKFYLMIQPEKGPQWLGHNPLQQLSYTGLYGVALVQAITGFAMY
                     GQSRPGGFWYTLFGWVVPMLGGIQIVHFVHHILTWVFLIFIPVHIYLALRADLLERTG
                     TISSIISGGRFVRADVEYVDRPE"
     gene            1543..2064
                     /locus_tag="AC16JC_07870"
     CDS             1543..2064
                     /locus_tag="AC16JC_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HIR5164648.1"
                     /translation="MWTDPSKTPAGRTVVIGLGNPLMADDGVGIAALERLRAAGAPAG
                     VELVDGGTWGMNLLPVIEAAGKLLLMDAIDAGGEPGTLHVLERARIPRYLATKISPHQ
                     VDLCDVLALTELRGTLPEDTVAIGLQPARVEMSCALSDAVRIRVDDLVAAVTHRLAEW
                     GHGVQPPAAPAHA"
     gene            2057..2317
                     /locus_tag="AC16JC_07875"
     CDS             2057..2317
                     /locus_tag="AC16JC_07875"
                     /inference="COORDINATES: protein motif:HMM:NF013334.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase/urease maturation nickel
                     metallochaperone HypA"
                     /protein_id="HIR5164649.1"
                     /translation="MHELGLALEVCRMAEERLGSDALPSVRAIGIEVGDDAGIEVGNF
                     QFCLETLLDEPPFRGARPLILRRPGPVLRLAYLEVDDGRPDD"
BASE COUNT          372 a          779 c          833 g          395 t
ORIGIN      
        1 ctctccaagg tcctccccta cgatcacatg aaggtgcagg agtacatcaa cagctcgtgg
       61 tacgagtatt ccaagggtga cgaggccggg ctgcatccct acgaggggga gaccaaggcg
      121 aaatacaccg gtcccccgac cccctggacc tacctgcagg accagcagaa gtacacgtgg
      181 atgaaggcac cgcgctacga tgggcagccg atggaagtgg gaccgctggc gcgcatgctc
      241 gtcgcctacg cgtcgggcca caaggaagtg caggagatgg tgggggcgac gctcaagggc
      301 ctcaacgtcg ggccggcggc gctgttctcg accctgggcc ggaccggagc acggggtatc
      361 gaaaccgtgt tgctcgcccg gcggctgtcg gtgtggtacg acaggctcgt ggccaacatc
      421 aagaacggcg acacccgcac cttcgacgat tcgaagtggg accccgccag atggcccgcg
      481 aaggcgcagg ggtacggcta cctcgacgcg ccgcgcggcg cgttggggca ctgggtgcag
      541 atcgaggacc agaagatcgc ccgctaccag tgcgtcgtcc ccagcacgtg gaattgctcg
      601 ccccgggacg ggaagggtgt gccgggaccg tacgaggcag cgctcatgga ccaccatcca
      661 ctggtcgatc ccgcgcggcc gatcgagatc ctgcgcacca tccattcgtt cgacccctgc
      721 atggcctgcg gggtgcacgt gctcgacgcc accggccgcg aggtcacgtc ggtgagggtc
      781 cagtgagcgc ccctcccggc aaggggccgc ggcccgcacc gcggccgagt ggggcgatgc
      841 gcggaatctc actcgcgcac aaggtgccgc cgccatcggg cgactaccac tgggtgtacc
      901 tgtggggctg gcccatccgt gccatgcact ggctcgccgt ggcgtgcatc gtggtgctcg
      961 tggtcaccgg cttctacatt ggccggccct acttcgtgac cagcggcgaa gcgagctcgc
     1021 attacctgat ggggtgggtg cggttcctcc acttcgcctc ggccggcgtg ctcgtggcga
     1081 ctgcgatagt ccggacgtac tggctgttcg tcggcaaccg cttcgagcgc tggcgggcgc
     1141 tgttcccgtt tagtccccga gactggggca gactatggaa gcaggtgaag ttctacctca
     1201 tgatccagcc cgagaagggt cctcagtggc tggggcacaa cccgctgcag cagctcagct
     1261 acaccggtct gtacggggtc gcgctggtcc aggccatcac tggattcgcg atgtacggcc
     1321 agtcgcgccc gggaggcttc tggtacaccc tgttcgggtg ggtcgtcccc atgctcggcg
     1381 gcatacaaat cgtgcacttc gtccatcaca tcctgacgtg ggtgttcctg atcttcatcc
     1441 cggtgcacat ctatctggcg ctgcgggccg atctgctgga gcgcacggga acgatctcct
     1501 cgatcatcag cggcggacgc ttcgtccgag ccgacgtcga gtatgtggac cgacccgagt
     1561 aagactccgg ccggccggac cgtcgtcatc ggcctgggca atccgctcat ggccgatgac
     1621 ggcgtgggca tcgcggcgct cgagcggctg cgggcggccg gggcgccggc cggcgtcgag
     1681 ctggtggacg gcgggacctg gggcatgaat ctgctccccg tcatcgaggc cgccggcaag
     1741 ctgttgctga tggatgcgat cgacgccggc ggggagccgg gaacgctgca cgtactggag
     1801 cgggcacgca tcccgcgcta tctggcgacc aagatttcgc cgcatcaggt ggatctgtgc
     1861 gacgttctgg cgctgaccga gctgcgcggc acgctccccg aggacaccgt cgccatcgga
     1921 ctgcagccgg cccgcgtgga gatgtcctgt gcgctgagcg acgccgttcg catccgggtg
     1981 gacgacctcg tggccgccgt cacgcatcgc ctggcggagt ggggccacgg ggtccagccg
     2041 ccggcggcac cggcgcatgc atgagctcgg cctggcgctc gaggtctgcc gcatggccga
     2101 agagcgcctc ggctcggacg ccctcccgtc ggtgcgcgcc atcggcatcg aggtcggtga
     2161 cgacgccggg atagaggtcg ggaacttcca gttctgtctg gagacgctgc tggatgaacc
     2221 accgtttcgc ggcgcgcgtc cgttgatcct gcgccggccc ggtccggtgc tgcgcctcgc
     2281 gtatctggag gtggacgatg gccgtccgga cgattgacgt tcgcgagcgg atcatggcca
     2341 gaaacgacga gctcgccgag caggtccgcg agcgcctgc
//
LOCUS       DBNGZW010000331         3477 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_509387, whole genome shotgun sequence.
ACCESSION   DBNGZW010000331 DBNGZW010000000
VERSION     DBNGZW010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_509387"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..593)
                     /locus_tag="AC16JC_07880"
     CDS             complement(213..593)
                     /locus_tag="AC16JC_07880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR5164650.1"
                     /translation="MKSSRGFTLIELLIVVVIIGILASIAIPKFANTKEKTYIAAMKS
                     DLRNLATAEEAYVFDSLTYTSTLSALNGWSPTTGVTVVVGVATGGGWSATASHLQTTR
                     KCYLFYGNAPPLGSATAEGKISCS"
     gene            complement(679..>3477)
                     /gene="polA"
                     /locus_tag="AC16JC_07885"
     CDS             complement(679..>3477)
                     /gene="polA"
                     /locus_tag="AC16JC_07885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HIR5164651.1"
                     /translation="MPDDSSRERLFLIDGYALIYRAFFAMIARPLTTQRGENTSAAWG
                     VVNFLLRLLQRHRPAYLAWVHDRGESFRHREYPDYKATREKLSLELQQDFDRSVERIV
                     DLLAAFRIPVLGVEGFEADDVIGTLAPQAAALGLQAVIVSGDKDFYQLIGPGVALLNP
                     GRGGPAAVEEQWVDESNASERFGIPPAQVVDYLALVGDSSDNVPGVKGIGEKTAVELL
                     RTYGDLDAMLARAAEVPGKRAREALLAHADAARLSRDLVTIRRNVPIMFDADAMRVAD
                     PDRAELERLFIELEFRSLIPKLEELAPPAAGAPPTAAPAVADEGGAAPATVVSDVAAL
                     ASVIAECRSAPLVAIDTEASSLDPMRARLVGMSLAVSGTRSWYLPFRHVAPDGPLAGG
                     VAPANLPPLDDDALRPLRDLLADPGVPKAGHHIKYDWLVLRRSGVELAGVGYDSMLAA
                     FVLDPGRRSFALDELARDRLNVRLRTYGDVAGRGRSEREFAAVPLGVAARYCCDDSET
                     VLRLREALAIELVDHRLLPLLQTVEMPLVPVLVDMEWAGVPVDRARLGELSRAFAVEL
                     AELERRLYAAAGTEFNLNSTPQLRTVLFTKLQLPIIKKTKTGPSTDVDVLEQLAAMGH
                     EVPRLLIEYRELSKLKSTYVDALPSYIHPETGRIHTSFNQVGAATGRLSSAEPNLQNI
                     PVRTERGESIRRAFIAPPGARLLVADYSQIELRLLAHFSGDPAFVQAFEKGGDIHRQT
                     AAVIFGVPQDQVTAEMRARAKTINFATIYGQGPFALARQLGIAQEEAKAFIRQYFERF
                     AGVRAWLDRTVLEARERGYVETLFGRRRYIPELKDRNFSIRAFGERTATNSPLQGSAA
                     DLIKVAMIRIHATLREQGLGSRLLLQVHDELVLEVPEQEEATAKDLVKRHMEGAAKLR
                     VPLVVSVGVGSNWVDAKG"
BASE COUNT          563 a         1243 c         1138 g          533 t
ORIGIN      
        1 ccggccggtg cggggatccc tgagcggctt gtactgcagc cagtcggcat gcagacccag
       61 cgcgcggccc agtaccagca gccgttcccg gtccgcgctc acgagatgca ccatcggctc
      121 cccgcgcaca cgccagcgat gcagccaaac gccgccgtcg agagcgaaac acatgccctc
      181 gcggcggcgt gtgaactcct gccagaaccc gctcagctgc agctgatctt gccctcggcg
      241 gtcgccgagc ccagcggcgg cgcgttgccg tagaacaggt aacacttcct ggtggtctgc
      301 aagtgcgacg cggtggccga ccagccgccg cccgtcgcca caccgacgac gaccgtcact
      361 ccggtcgtcg gcgaccagcc gttgagcgcg gagagggtac tcgtgtacgt gaggctgtcg
      421 aagacgtagg cctcttccgc cgtcgccagg ttgcgcaggt cggacttcat cgcggcgatg
      481 tatgtcttct ccttcgtgtt ggcgaacttc gggatcgcga tggacgcgag aatcccgatg
      541 atcaccacca caatcaggag ctcgatcaat gtgaaaccgc gggacgactt caccacaaca
      601 ccctccaggc ctctgtgggc gccccaaccc tacgagtact gagcgacggg gcataggtcc
      661 gtaacgcaag acctgtgctc agcccttggc atcgacccaa ttgcttccca caccgacact
      721 tacgacgagc gggacacgca acttcgcggc cccctccata tggcgtttta ccaaatcctt
      781 cgcggtagct tcttcctgct ccggcacttc caacaccagc tcgtcatgga cctggagcag
      841 cagccgtgac ccgagcccct gttcccggag cgtggcgtgg atccggatca tggcgacctt
      901 gatcaggtcc gccgcggatc cctggagcgg agagttcgtc gccgtccgct ccccgaacgc
      961 ccggatactg aagttcctgt ccttcagctc cgggatatag cggcgccgcc cgaacagcgt
     1021 ctcgacgtac ccccgctcgc gcgcctcgag cacagtccgg tcgagccaag ctcgtacccc
     1081 cgcgaaacgc tcgaagtact gtcggatgaa cgccttggct tcctcctgag cgatgccgag
     1141 ctgccgcgcc agggcgaacg ggccctggcc gtagatcgtg gcgaaattga tcgtcttggc
     1201 gcgcgcgcgc atctccgcgg tgacctggtc ctgcggcacg ccgaagatca ccgccgccgt
     1261 ctggcggtgg atgtctcccc ccttctcgaa cgcctggacg aaggccggat cgccggaaaa
     1321 atgggccagc agacgcaact cgatctggga gtagtcggcg acgagcagcc gggctccggg
     1381 gggcgcgatg aaggcccgtc ggatcgattc cccccgctcc gtgcgcaccg ggatgttctg
     1441 gaggttcggc tcggccgagg acaggcgccc ggtggccgcc ccgacctggt tgaagctggt
     1501 atgaatgcgg ccggtctcgg gatggatgta gctcgggagg gcgtcgacgt aggtggattt
     1561 cagcttcgac agctcgcgat actcgatcag cagtcggggc acctcgtgcc ccatcgccgc
     1621 gagttgctcc aatacgtcca cgtcggtgga ggggccggtc ttcgttttct tgatgatggg
     1681 cagctgcagc ttggtgaaca ggaccgtgcg cagctgcggc gtggagttga ggttgaactc
     1741 cgtgccggcg gccgcgtaca gtcggcgctc gagctccgcc agctccaccg cgaacgcccg
     1801 cgacagctcg cccaaccggg cgcggtccac cggcacgcct gcccactcca tgtccaccag
     1861 gaccggcacc agcggcatct ccaccgtctg gagcaggggc agcaggcgat ggtccaccag
     1921 ctcgatcgcc agcgcctctc gcaggcgcaa cacggtttca ctgtcgtcgc agcaataccg
     1981 cgccgcgacg ccgagcggta ccgccgcgaa ctcccgctca cttcgtcccc gccccgctac
     2041 atcgccgtag gtgcgcagcc ggacgttcag gcggtcgcgc gccagctcgt ccagggcgaa
     2101 cgagcgccgg ccgggatcca ggacgaacgc ggccagcatg gagtcatagc ccacgcccgc
     2161 cagctccact ccgctgcgcc gcaggacgag ccagtcgtac ttgatgtgat ggcccgcttt
     2221 cggcacgccg ggatcggcca gcagatcgcg cagcgggcgc agcgcgtcgt cgtccagcgg
     2281 agggagattg gccggggcca ccccgcccgc gagtggccca tcgggcgcca cgtgcctgaa
     2341 aggcaggtac caggagcgcg tgcccgacac cgccagcgac atgcccacga gccgggcgcg
     2401 catggggtcg aggctggagg cctcggtatc gatcgccacg agcggcgcgc tgcggcactc
     2461 cgcgatcacg ctggcgagcg ccgccacgtc cgatacgacc gtggcgggag ccgcccctcc
     2521 ctcgtcggcg accgccggcg ccgcggtcgg cggcgcgccg gccgcggggg gcgccagctc
     2581 ctcgagcttc gggatcagcg accggaactc cagctcgatg aacaaccgct cgagctcagc
     2641 gcgatcgggg tccgccacgc gcatcgcgtc ggcgtcgaac atgatcggca cgttccgtcg
     2701 gatcgtgacg agatcgcgcg agagacgcgc cgcgtcggcg tgggcgagga gcgcctcacg
     2761 cgcccgcttg cccgggacct cggctgcccg cgccagcatc gcatccaggt cgccgtacgt
     2821 ccgcagcagc tcgacggccg tcttctcccc gatccccttc acgcccggca cgttgtccga
     2881 gctgtcgccc acgagcgcga ggtagtccac cacctgcgcg ggcgggatgc cgaaccgctc
     2941 gctcgcattg gactcgtcga cccattgctc ctcgaccgcc gcgggtccgc cccgtccggg
     3001 gttcaggagc gcgacccccg gaccgatcag ctgatagaag tccttgtcgc cggagacgat
     3061 caccgcctgc aggccgaggg ccgccgcctg cggggcgagg gtgccgatca cgtcgtccgc
     3121 ctcgaacccc tcgacgccga ggaccgggat gcggaacgcc gcgagcagat ccacgatgcg
     3181 ctccaccgaa cggtcgaagt cctgctgcag ctcgaggctg agcttctcgc gcgtggcttt
     3241 gtagtcgggg tattcccgat ggcggaacga ttccccccgg tcgtgtaccc aggccaggta
     3301 ggccggtcga tggcgttgca gcaggcggag gagaaaattc acgacccccc acgcggcgga
     3361 cgtgttctcc ccgcgctgcg tggtgagcgg ccgcgcgatc atcgcgaaga acgcacggta
     3421 gatgagcgcg tacccgtcga tcaggaagag acgctcgcgc gacgagtcgt ccggcat
//
LOCUS       DBNGZW010000332         2441 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1664974, whole genome shotgun sequence.
ACCESSION   DBNGZW010000332 DBNGZW010000000
VERSION     DBNGZW010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2441
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1664974"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="AC16JC_07890"
     CDS             complement(<1..762)
                     /locus_tag="AC16JC_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5164652.1"
                     /translation="MNYLLLHPDSWSPAPLVPALETARVETRGIREAADLLPEERPTV
                     FLLPKDGRHTFPLTALRTFVDAGGAIVALGTDGEGDVPECLPTELLSGFVPHPAGVRQ
                     LLVAIRAAYREAAARAEASRARREASLRGHEVAELTGIGTKLMTERDPEQLLDDILTQ
                     ARRLTQSDAGSLYLIETDVFGAKGLRFRLAQNLTLPNVPLVQFTMPLDRTSLAGYAAV
                     TGEPLVIDDAYLLPPDVEYTINRSFDERHGYRSRSM"
     gene            complement(759..1688)
                     /locus_tag="AC16JC_07895"
     CDS             complement(759..1688)
                     /locus_tag="AC16JC_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR5164653.1"
                     /translation="MRGSVDTTRQDFIVGLVLVGAIAVIVGALLATSGWGERRYDLFL
                     RAASAEGLTADSKVLLQGLEVGRVMRVSPRVDSTTRTVSFVAKLSMIERFSGGADLRL
                     PAGTRGRLEPASQISSTVLIRIVLPDSGGRAARLLAEGDTIESDRKVPVMDQVAEVAQ
                     QLSKEVQEVLHRTHQSLERVDGILAQANGVLKDIRPSVETTLADVSTTMGHVNKLVQR
                     LDPGLADSVSRAIALSNSVLLRVDSLAAQAGKMTTENREDVRIAVANLTQLTRQLNHL
                     AEQVSRRPYRLLTGVKPLPSDSAPRAGTQDSIP"
     gene            complement(1688..2368)
                     /locus_tag="AC16JC_07900"
     CDS             complement(1688..2368)
                     /locus_tag="AC16JC_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5164654.1"
                     /translation="MIAPPLVFQDLPLTERSRATFSLTLASHETIAVLGDEESGVDQL
                     GGYALGLESPPGGRVLMFDTAIAELPLPQRLAFRHRVGYLPAGDGLLQNLSLRDNIAL
                     PLRFGSDARSREIDGRVAVIVAALGLGGAADRRPAQATEEECRRAALARAVAFDPTLL
                     VLEQPFDGITARAAAELLEIARGGETAEGARRTILITGQEIPTVVQRRCDRVYRLTRA
                     GTLELETV"
BASE COUNT          369 a          852 c          863 g          357 t
ORIGIN      
        1 catcgaccgc gagcggtagc cgtgccgctc gtcgaaggag cggttgatgg tgtactcgac
       61 gtcgggtggg agcagatacg catcgtcgat caccagcggc tcgccggtca cggccgcgta
      121 ccccgcgagg ctggtgcggt cgagaggcat cgtgaactgc accagcggca cgttcggcag
      181 ggtgaggttc tgcgccagcc ggaaccgcag tcccttcgcg ccgaagacgt cagtctcgat
      241 cagatagagg ctgccggcat ccgattgcgt gagccgccgc gcctgcgtca ggatgtcgtc
      301 gagcagctgc tccgggtcgc gctcggtcat cagcttggtg ccgatccccg tcaattcggc
      361 gacctcgtgg ccgcgaagcg acgcctcgcg gcgggcgcgg gaggcttcgg cgcgcgccgc
      421 ggcctcccgg taggccgccc ggatggccac gagcagctgg cgcacgccgg ccggatgggg
      481 cacgaatccg ctcaacagct cggtcgggag acactcgggc acgtcgcctt ctccgtccgt
      541 gcccagcgcg acgatcgcgc cgccggcgtc cacgaacgtc cgcaacgcgg tgagcgggaa
      601 cgtgtgccgg ccgtccttgg ggagcagaaa gacggtgggg cgttcctccg gcagcagatc
      661 cgcggcctcc cggatgcccc gtgtttccac gcgggcggtt tccagcgccg gcacgagcgg
      721 cgccggagac cagctgtccg ggtgcagcag cagatagttc acgggatgct gtcctgggtc
      781 ccggcgcgcg gtgcggaatc ggacggcagc ggcttcaccc cggtgagcag gcggtacgga
      841 cggcgcgaca cctgttcggc gaggtgattg agctggcggg tcagctgggt gaggttggcc
      901 accgcgatgc ggacgtcctc gcgattttcg gtcgtcatct tgcccgcctg ggccgcaagg
      961 gaatcgacgc ggagcaggac gctgttcgac agggcgatgg cacgcgacac cgagtcggcg
     1021 agtcccgggt cgaggcgctg gaccagtttg ttgacgtggc ccatcgtcgt cgagacgtcg
     1081 gcgagcgtcg tctccacgct gggccggatg tccttcaaca cgccgttggc ctgggccagg
     1141 atgccgtcca cgcgctccag cgactggtgg gtgcgatgca gcacctcctg gacctccttg
     1201 gagagctgct gcgccacttc ggcgacctgg tccatcacgg ggactttccg gtcggactcg
     1261 atggtgtccc cctcggcgag cagccgggcg gcccggccgc ccgaatcggg gaggacgatc
     1321 cggatcaaca ccgtcgagga gatctggctg gccggctcga ggcgtccccg ggtgccggcc
     1381 ggcagccgca ggtccgcacc gcccgagaag cgctcgatca tcgacaattt cgccacgaag
     1441 gatacggtcc gcgtggtgga atccacccgg ggagacaccc gcatcacccg gccgacttcc
     1501 agtccttgca gcaatacctt gctgtcggcc gtcagccctt cggcgctcgc ggcgcggagg
     1561 aagaggtcgt agcggcgctc gccccacccg ctcgtcgcca ggagcgcccc cacgatcacg
     1621 gcgatcgcgc ccacgagcac gagccccacg atgaagtcct gccgggtggt gtccacggag
     1681 cctcgcatca gaccgtctcc agctcgagcg tcccggcgcg tgtgagccgg tagacgcgat
     1741 cacagcggcg ctgcacgacc gtcggaattt cctggccggt gatgagaatg gtgcggcgtg
     1801 cgccttccgc cgtctccccg ccgcgcgcga tctccagcag ctccgccgcc gcgcgggccg
     1861 tgatcccgtc gaagggctgc tccagcacca gcagagtcgg gtcgaaggcc acggcccgcg
     1921 cgagcgcggc acgccggcac tcctcctccg tcgcttgtgc ggggcggcgg tcggccgctc
     1981 cacccagccc cagggcggcc acgatcacgg cgacgcggcc gtcgatctcc cgcgagcgcg
     2041 cgtcgctccc gaaccgcagt ggcaatgcga tgttgtcacg cagggagaga ttttgcagca
     2101 gaccgtcgcc cgccggaagg tacccgaccc ggtgccggaa tgccaagcgc tgcggcaggg
     2161 gaagctcggc gatggccgtg tcgaacatca gcacccgtcc ccccggcggc gactccagtc
     2221 ccagcgcgta cccgccgagc tgatcgactc cgctctcctc gtcgcccagg acggcgatcg
     2281 tctcgtgcga agcgagcgtg agcgaaaacg tcgcccggct ccgctccgtg agcggcaggt
     2341 cctggaacac gagcggggga gcgatcatgc gctcagccca gcagcacgaa cagggcggag
     2401 cacaggaaca cgccgacgat cgatgtgacc gtggcgcggc t
//
LOCUS       DBNGZW010000333         2475 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_642314, whole genome shotgun sequence.
ACCESSION   DBNGZW010000333 DBNGZW010000000
VERSION     DBNGZW010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_642314"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..124
                     /locus_tag="AC16JC_07905"
     CDS             8..124
                     /locus_tag="AC16JC_07905"
                     /inference="COORDINATES: protein motif:HMM:NF018439.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1207 domain-containing protein"
                     /protein_id="HIR5164655.1"
                     /translation="MLDRNLQLLVEYFHGNSPDGQFFKRRVEYLGLGAHFHF"
     gene            complement(632..1042)
                     /locus_tag="AC16JC_07910"
     CDS             complement(632..1042)
                     /locus_tag="AC16JC_07910"
                     /inference="COORDINATES: protein motif:HMM:NF019934.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HIR5164656.1"
                     /translation="MHNSTEVQAQRARRRQRGFTLIELMIVVAIIGILAAIAIPLYAN
                     VQARARLAKAQADARALASAVSIYSAHTGTLPTALVDLTSTVTNALGQPGGPFMASIP
                     GGPAGWGTYVYTPVATAGTFTITNTGDSTSVSVP"
     gene            1559..1888
                     /locus_tag="AC16JC_07915"
     CDS             1559..1888
                     /locus_tag="AC16JC_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HIR5164657.1"
                     /translation="MTAPCVFCRIAAKESPAEIAYEDDDVLAFRDLYPKAPIHLLIIP
                     KRHIESIARLEPGDAPLIGRCVHVARLLGERTGYGERGYRLSCNTGPEGGQVVYHVHF
                     HFTAGRR"
     gene            1885..>2475
                     /locus_tag="AC16JC_07920"
     CDS             1885..>2475
                     /locus_tag="AC16JC_07920"
                     /EC_number="7.3.2.2"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HIR5164658.1"
                     /translation="MIRVRGLRKDYPGGSRALHDVDLDIGPGEFVALIGPSGAGKSTL
                     LRCLNGLVTPTAGRVEVGGVAVTGATPETLRRVRAGVGFVFQQFNLLRRLTVLENTML
                     GRLSHSARWRSLVGWFDPADLARARATLARVGLDGLDTRRIDTLSGGQQQRVAIARAL
                     VQAPAVILADEPMASLDPSLAHTVMELLRRINQEDGI"
BASE COUNT          382 a          837 c          855 g          401 t
ORIGIN      
        1 cgtccgggtg ctcgaccgca acctgcagct gctcgtcgag tacttccacg gcaactcccc
       61 ggacgggcag ttcttcaagc gccgcgtcga gtacctgggg ctcggagccc acttccactt
      121 ctagcgccca atctcaaatg gcttgacagg ggacggtcgg cctgccgcct cccgtccggc
      181 gtggcggccg gcccgcatcc tagcagttca cccttttgcc gccgcgtcgc gaaattttcc
      241 cgttcgcagg ccccccgggg tgcgggtgga gacgatcggg gacaaagaag gctcgggcgt
      301 gggcagtccc aggaaacagg attcctagga gcctcgatcg gtcccggcgg gggtgggggc
      361 gcggggacgc gccgcgggcg ccggcactgc ggttgcgccg ggttgcgccg gttgcggctc
      421 atgggcaccg cagggcaggg aaggctgggg ctagtccact cactggcggg catctgcgcg
      481 aggtaccgag ggccgcgcca tacgaatccc ttaccctcca ccatgaattc ctcacgcgcg
      541 acgatctcgc gtcgtcgatt gccggggcag ggtccgaaaa gtaaaacggc cggggctcga
      601 tgccccggcc gttggtctta gcagcgtcgc gttacggtac gctgacgctg gtgctgtcac
      661 ccgtgttggt aatcgtgaag gtgccggccg tcgccaccgg agtgtagacg taggtacccc
      721 agccggccgg gccccccgga atgctggcca tgaacggacc gcccggctga cccagcgcgt
      781 tggtcaccgt cgacgtgaga tcgaccaggg cggtgggaag cgtgccagtg tgcgccgagt
      841 agatgctgac ggcggacgcg agcgcccgcg cgtcggcctg ggccttcgcg agacgcgcgc
      901 gcgcctgcac gttggcgtac agcgggatgg cgatcgcggc gagaatgccg atgatggcca
      961 ccacgatcat caactcgatg agcgtgaagc cgcgctggcg gcgccgagcg cgctgtgcct
     1021 gaacctcggt tgagttgtgc atgagccttc gctcctccta caagagagaa taggacgttc
     1081 ttacagactt aggcttcgga cttacaagac tcgggccact ctcggaaggg ccagatcgac
     1141 cccggacgta ggctctggaa cgtcgcgtca agttcgtaca gtcaagcatt tacggtccga
     1201 tgcccaggtt ccggtcagag tcgaccccgg tgcaggtgac agattcgggc atggttccgt
     1261 gacgagggca ggcgtcatcg gcaacatcgc cgacgtcggc gaccgatcag ccgaggccga
     1321 cccgcgcgct ggatcggctg cggatccggc gaccgacgaa cgtgggtgga gaagcggggg
     1381 cgggagagtc taggcgcgct tcgcggcggc agtgggcttg ggcgggcaca tcttgtggac
     1441 ccagacggac cccagcttga cgtggttgcc gttcttccgg atccgaagct tgcacttggg
     1501 gcaggtgccc atgtcattct cctttgcgtc gagcagtata cacgagccgg gaggagccgt
     1561 gaccgcgccc tgcgtgttct gtcgcatcgc cgccaaggaa agcccggccg agatcgcgta
     1621 cgaggacgac gacgtcctcg cgttccgcga tctgtatccg aaggcaccga tccacctgct
     1681 gatcatcccg aagcgacaca tcgaatccat cgcgcgcctc gagcccggcg atgccccgct
     1741 gatcgggcgc tgcgtccacg tcgcgcgcct gctgggggag cgcaccggct acggtgagcg
     1801 cggctaccgc ctctcgtgca ataccggacc ggagggcggg caggtcgtct accacgtcca
     1861 cttccacttc accgccggcc gccggtgatc cgggtccggg gccttcgcaa ggactatcca
     1921 ggcggctcgc gggcgctgca cgacgtggac ctcgacatcg gcccggggga gttcgtggcc
     1981 ctgatcgggc cgagcggcgc ggggaagtcc acgctgctgc ggtgcctcaa cggtctcgtg
     2041 acgcccaccg cggggcgagt cgaggtcggt ggcgtcgcgg tgaccggggc gaccccggag
     2101 acccttcgcc gcgtccgggc cggcgtcgga ttcgtgttcc agcagttcaa cctcctgcgc
     2161 cgtctcaccg tgctcgagaa cacgatgctc ggccggcttt cccattccgc gcgatggcgc
     2221 tccctcgtcg gatggttcga cccggccgac ctggcccgcg cccgcgcgac gctcgcgcgc
     2281 gtcggcctcg acggcctcga cacccgccgc atcgacacgc tctccggcgg ccagcaacag
     2341 cgggtggcca tcgcccgggc cctggtccag gcgcccgcgg tgatcctggc cgacgagccg
     2401 atggcgagcc tcgatccctc gctggcccac accgtcatgg aactactgcg ccggatcaac
     2461 caggaagacg gcatc
//
LOCUS       DBNGZW010000334         1866 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1872908, whole genome shotgun sequence.
ACCESSION   DBNGZW010000334 DBNGZW010000000
VERSION     DBNGZW010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1872908"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..514)
                     /locus_tag="AC16JC_07925"
     CDS             complement(<1..514)
                     /locus_tag="AC16JC_07925"
                     /inference="COORDINATES: protein motif:HMM:NF047111.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5164659.1"
                     /translation="MSRLRPLLLAAVVAGVAACSDDAVQPDTNSTPADLSLRGLGVST
                     GPEQVMPGEVIVRLKAGADAATVGKAHGLTLSGRGYKDAFVVLKGAVGAEHAAAEALA
                     RDARVAYAEPNYLREPTATPINPSLWAFYNPGGLNMKFTSGRSKGQSLPSSYASTSDA
                     DEDGAPGGNAG"
     gene            700..>1866
                     /gene="dinB"
                     /locus_tag="AC16JC_07930"
     CDS             700..>1866
                     /gene="dinB"
                     /locus_tag="AC16JC_07930"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR5164660.1"
                     /translation="MGARILHVDLDAFFVEVCRQRHPELREMELLVVGGRRDQRGVVQ
                     SASYGARRYGIHAGMPIAQAVRLCPQATFFQGAFPDYRDASRAVHVVLERFSPTVVMA
                     SLDEAYLDFAGTERLYPVSLLPIAEQLRDEVRRDTGLDCSVGIGPNRMIAKLASDAAK
                     PRGLMEVRAGWEEGFLAGLALKAMPGVGPKTAARWVDLGLVDVWQVQQMDEAALERLI
                     GPEARALKRRVHGYGGTTLRGGRLPRSVSRETTLSRDLRDPASLEATLSLLTARVAGQ
                     LRDEHLVARTVTLKLRHDDFRTVTRRHTLESATDLDAELYAAARALFREAFADVRGRN
                     RGVRLIGIAATNLGVAAEADLFEPPARRRLRELSAAVDHLREKYGFGAVTPGSAL"
BASE COUNT          272 a          670 c          647 g          277 t
ORIGIN      
        1 cgccggcatt gccccctggc gctccgtcct cgtcggcatc ggaggtcgag gcataggaag
       61 acggcagcga ctggcccttc gatctgccgc tcgtgaactt catgttcagc ccgccgggat
      121 tgtagaacgc ccagaggctg gggttgatcg gcgtcgccgt cggctctcgc aggtagttcg
      181 gctcggcgta cgccacgcgc gcgtcccgcg cgagcgcctc ggccgccgca tgctccgctc
      241 cgaccgcacc cttcagcacc acgaacgcgt ccttgtaccc gcgcccgctc agggtcaggc
      301 cgtgcgcctt gccgaccgtc gccgcgtcgg cgcccgcctt gagcctcacg atgacttccc
      361 ccggcatgac ctgctcgggt ccagtggaaa cacccaaccc ccgcagggaa aggtcggcag
      421 gggtcgaatt ggtgtcgggc tggacggcgt cgtccgaaca ggcggcgaca ccggccacga
      481 cggcggccag cagcagcgga cgcagacgac tcatgacatc tccttcgagg tgaacggcca
      541 cgcgtgaaga gccaaattgg gcgtcgatcg gatggtgcgg gaaggccgga ccggacctgc
      601 attctgaaac aactcaacgg tacctgggcc cgcaagccgt gcagcccgtt gcacgcctgt
      661 tcaggtcctc accggcgcca aggggactaa tttccggtca tgggcgcccg catcctccac
      721 gtcgacctcg acgcgttctt cgtcgaagtg tgccgccagc ggcatcccga gctccgggag
      781 atggagctcc tggtggtcgg cggccggcgc gaccagcgtg gggtggtcca gtcggcctcg
      841 tacggcgccc gccgctacgg catccacgcc ggcatgccga tcgcgcaggc cgtccggctc
      901 tgtccccagg ccaccttctt ccagggcgcc tttcccgact accgagacgc ctcccgcgcc
      961 gtccacgtcg tgctcgagcg gttcagcccg acggtcgtga tggcgtccct cgacgaggcc
     1021 tacctcgact tcgccggcac cgagcgattg tatccggtct cgctgctgcc catcgccgag
     1081 cagctccgcg acgaggtccg gcgcgacacg ggcctcgact gcagcgtcgg gatcggtccc
     1141 aaccggatga tcgccaagct ggcttccgat gcggcgaagc cgaggggcct gatggaagtg
     1201 cgcgccgggt gggaggaggg ctttctggca ggactcgcgc tcaaggcgat gccgggtgtg
     1261 ggacccaaga cagccgcgcg ctgggtcgat ctgggactgg tggatgtctg gcaggtgcag
     1321 cagatggacg aggcggccct cgagcgactg atcggccccg aggcgcgggc gctcaagcgt
     1381 cgggtgcacg ggtacggcgg cacgacactc cggggcgggc ggctgccccg gtcggtgagc
     1441 cgcgagacga cgctctctcg ggacctgcgc gaccccgcga gcctcgaagc cacgctctcc
     1501 ctgctcacgg cgcgcgtggc gggacagctc agggacgagc atctggtcgc ccggaccgtc
     1561 acgctcaagc tccggcacga cgacttccgc accgtcaccc ggcgccacac gctcgagagc
     1621 gcaaccgacc tcgacgcgga gctctacgcg gcggcgcggg ccctgtttcg cgaagccttc
     1681 gccgacgtgc gcgggcggaa tcggggcgtc cgcttgatcg gcatcgcggc caccaatctc
     1741 ggcgtcgccg ccgaggccga cctgttcgag ccgccggcgc ggcggcggct ccgcgagctg
     1801 agcgcggcgg tggaccatct gcgcgagaag tacggattcg gcgccgtgac ccccgggagc
     1861 gcgctc
//
LOCUS       DBNGZW010000335         6599 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1665280, whole genome shotgun sequence.
ACCESSION   DBNGZW010000335 DBNGZW010000000
VERSION     DBNGZW010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6599)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6599)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6599
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1665280"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(18..1127)
                     /locus_tag="AC16JC_07935"
     CDS             complement(18..1127)
                     /locus_tag="AC16JC_07935"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HIR5164661.1"
                     /translation="MTDHLAVGLMSGTSLDGVSTALVRLDAAASAAQLVAFRQEPYSS
                     AERGAIIEAIARGGAKELSLLHVALAERFAGAALQLLAAARVSPRDLSFVASHGQTIW
                     HEPGRATLQLGDPAVLAERLGVRVVSDFRSRDVAAGGQGAPLVPLADVMLFGHPERGR
                     LLLNLGGMANVTWVPRRGETQGALAFDTGPGVAVIDAVTRRLDPEAAYDRDGERARRG
                     RVSRKALAALLADPYFEQAPPKSTGRERFGQAYSERVLELAGAGNDAVATATALTVES
                     VARALERWTPAQGSDELVISGGGARNPALVEQLAARVRPRPLRAFDELFFDGEAKEAV
                     AFAFLGLLTLLGKAGNLPAATGARGPRVLGHVTPA"
     gene            complement(1124..2068)
                     /gene="murQ"
                     /locus_tag="AC16JC_07940"
     CDS             complement(1124..2068)
                     /gene="murQ"
                     /locus_tag="AC16JC_07940"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA];
                     GO_process: GO:0046348 - amino sugar catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HIR5164662.1"
                     /translation="MADTPAFQDPRLTEQRNPRTERIDVASSLEIVDLLNAEDRTVPV
                     AVHGQREAIARAIDLVADALRTGGRLVYVGAGTSGRLGVLDAAECPSTFGTSPDLVVG
                     VIAGGYPALVKSAEGAEDDVNAGMAAMDTARVSPRDVVVGIAASGTTPFVRAALSRAQ
                     TIGARTVIVSCSPPPRLLVDTCDVTIVPLVGPEALTGSTRMKAGTATKLVLNTISTGA
                     MIRLGRAYGNLMVDLMALSAKLVDRGERIVMEACGVTRERARAAIEAADRSVKLAIVM
                     VRAGLAADEARRRLEAAGGFVRHAVGDPPPSPPPAAPQ"
     gene            2130..3230
                     /locus_tag="AC16JC_07945"
     CDS             2130..3230
                     /locus_tag="AC16JC_07945"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR5164663.1"
                     /translation="MNDAGRRWPVGVTVLTVLAVFYTLYFARAFLIPITFAVLLDFLL
                     SPAVRALVRWHVPLPVAAALVLFAMLGTGSLFVYDLSGPVQSWADNAPETLATAQTRL
                     RRLLKPLQRVTKTAEQVESAATGGVAGGGAAKPTEVVVRGPSIVSRLFGSTQRFLIGL
                     LEVTFLLYFLLAAGDLFLQKLIKVLPNVHDRHKAIEIARATEASISTYLLTTLAVNAV
                     EGGVVGAALWALGMPNPVLWGALVTLLEFIPYLGALSLVVLLTLGAFATFNDVGHALL
                     IPAAFLAINLIQANLVSPMLLGHRLALNPVALLVSLAFWLWIWGIPGGLLAVPLLATL
                     KIFCDHIERLAPVGEFLGRRDGELQPPILKSS"
     gene            3262..4968
                     /gene="ggt"
                     /locus_tag="AC16JC_07950"
     CDS             3262..4968
                     /gene="ggt"
                     /locus_tag="AC16JC_07950"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036374 - glutathione gamma-glutamate
                     hydrolase [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006751 - glutathione catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HIR5164664.1"
                     /translation="MRPRLLVSICCLAACARSGGLSHPAPTSTWELAGKAVAVTAPHA
                     MVTTGHPLATEVGRRVLEQGGNAIDAAVAVGFALTAVLPAAGNIGGGGFIVYRDSAGA
                     VSALDYREAAPAAASRDMFVDSTGEPTERSITGHLSAGVPGSVAGLYEAWRRFGKLPW
                     PALVAPAIALAEGHPVDSSRSSGLAGDSVRLSLFPASAAQFLVAGRVPQPGTVLHQPD
                     LARTLRLIADSGPDVFYRGQIADLIVAEMARGGGIITREDLAAYRPKWREPIRVEYRG
                     YAVYSMPPSSSGGVTLAEILNILGGYDSLPPFGSTALLHLETEAMRRAFEDRNHWLGD
                     PDFVQMPLDRLLSKSYAAELRAQIDPTRATPTPPFQAGPPGSTETTHYSIVDADGNAA
                     AVTTTLNNSYGSAVTVTGAGFLLNDEMDDFATAPGKPNMFGLVQGEVNAVAPGKRMLS
                     AMTPTIVLDPEGRLFLVAGAPGGPRIITAVYHAISNVIDHHMSLAQAIEAPRGHHQAL
                     PDTLMVERGGFNVAVLDSLRAMGHGVREWGYHAYVMGIERGPRGWVGVADPRSGGNAA
                     GY"
     gene            complement(5067..5870)
                     /locus_tag="AC16JC_07955"
     CDS             complement(5067..5870)
                     /locus_tag="AC16JC_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164665.1"
                     /translation="MPTNSKSSWTLSLGLVLGLVAAGACAKSDKTADQRADSAARNLT
                     LAPTESTAAMQDVPAPAAQPAPTPPPSRPAAPKTKPPAPRPTPAAAATRTAAAGSFLD
                     LAVSDTLSSRTTKVGDAFTASVVADVKDANGRVVIPAGSTVHGSVAAVKPAPNPSTPG
                     TLQLSLTSVSVGGTDYPLDAKIDSLETVRAGRGVTGGDVATVGAGAAAGAILGRVLGK
                     NAKGAIIGGVVGGAVGAGVATQTKDADIVLPKGAHINASLVSAVTVKVS"
     gene            complement(5980..6402)
                     /locus_tag="AC16JC_07960"
     CDS             complement(5980..6402)
                     /locus_tag="AC16JC_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164666.1"
                     /translation="MYHHSLHRAAAAVLGLSVLAACKKTAPPPAQAPVEVAAVDLGRQ
                     IGPDNRVVQSMTTFGVNDTIYASVATTGTATSAVLTAKWTYQTGQTVDSTTMTIAPTG
                     PAQTEFHIMKASPWPAGGYTLAIWLNGQSVGQKAFEVK"
BASE COUNT          887 a         2438 c         2270 g         1004 t
ORIGIN      
        1 ccccccactc ccctgtttca cgccggcgtt acgtgcccca gcactcgcgg ccctctcgct
       61 cccgtcgccg ccggcaagtt ccccgccttt cccagcagcg tcagcagtcc cagaaacgcg
      121 aacgccaccg cctccttcgc ttccccgtcg aagaacagct cgtcgaaggc gcgcagcggg
      181 cgtggccgca cccgcgccgc gagctgttcg accagcgccg ggttcctcgc cccgccaccc
      241 gagatgacga gctcgtccga cccttgagcc ggcgtccagc gctcgagcgc gcgggccacg
      301 ctctccaccg tcagggccgt cgcggtggcg acggcgtcgt tgcccgcccc cgccagctcg
      361 agcacgcgct cggagtaggc ctggccgaag cgctcgcgtc ccgtgctctt gggcggcgcc
      421 tgctcgaagt agggatcggc cagcagcgcc gccagcgcct tccgggagac ccggccgcgg
      481 cgcgcccgct cgccgtcgcg atcgtaggcg gcctcggggt cgaggcgccg cgtgaccgcg
      541 tcgatcaccg cgacgcccgg cccggtgtcg aacgcgagcg ccccctgcgt ctcgccacgg
      601 cgtggcaccc aggtgacgtt cgccatcccg cccaggttga gcagcaaacg cccccgctcg
      661 gggtggccga acaacatcac gtcggcgagc gggacgagcg gggcgccctg cccgcccgcc
      721 gccacgtcgc gcgagcggaa gtcgctcacc acccgcacgc cgagccgctc ggcgaggacg
      781 gccggatcgc cgagctgcag cgtcgcccgg cccggctcgt gccagatcgt ctggccgtgc
      841 gaggccacga aggagagatc gcggggtgat acccgcgccg cggcgaggag ctgcagcgcc
      901 gccccggcaa agcgctccgc cagcgccacg tgcagcaacg acagctcctt cgccccgccg
      961 cgcgcgatcg cctcgatgat ggcgccccgc tccgccgacg agtacggctc ctgccggaac
     1021 gcgaccagct gcgccgcact cgccgcggcg tcgagccgca ccagcgccgt cgaaaccccg
     1081 tcgagcgagg ttcccgacat caggcccacc gccaggtggt cggtcactgc ggggcggcgg
     1141 gcggtgggga cggcggcgga tcgcccaccg cgtggcgcac gaacccgccg gccgcctcga
     1201 gccggcgccg cgcttcgtcc gcggcgagac ccgcccggac catcacgatc gcgagcttca
     1261 cgctccggtc cgccgcctcg atcgccgcac gggcccgctc gcgggtcacg ccgcacgcct
     1321 ccatcacgat gcgctcgccc cggtccacca gcttggcgct cagcgccatg agatccacca
     1381 tcaggttgcc gtaggcccgg cccagccgga tcatcgcgcc ggtcgagatc gtgttgagaa
     1441 cgagcttggt ggcggtaccc gctttcatcc gggtcgagcc ggtgagcgcc tccggcccca
     1501 ccagcggcac gatcgtcacg tcgcaggtgt cgaccagcag ccgaggcgga ggggagcacg
     1561 agacgatgac cgtgcgcgcg ccgatcgtct gcgcgcgcga cagcgcggcg cgcacgaacg
     1621 gcgtcgtgcc gctggccgcg atgcccacga cgacgtcgcg cggcgacacc cgcgccgtgt
     1681 ccatcgccgc catccctgcg ttcacgtcgt cctcggcgcc ctcggcgctc ttcaccagcg
     1741 ccggatagcc ccccgcgatc acgccgacca cgaggtcggg gctcgtgccg aacgtcgacg
     1801 ggcactcggc cgcgtcgagc acgccgagcc gtcccgacgt gcccgcgccg acgtacacca
     1861 gccgtccccc ggtccgcaac gcgtccgcca cgaggtcgat cgcccgcgcg atcgcctcgc
     1921 gctggccgtg caccgccacc gggacggtcc ggtcctcggc gttcaggagg tccacgatct
     1981 cgaggctcga cgcgacgtcg atgcgctcgg tgcgcgggtt gcgctgctcg gtgaggcggg
     2041 gatcctggaa cgcgggggtg tcggccatga tgcgtggtgg gcaaaactag ggcgtggccg
     2101 cctgccccgt aagggtttat cgtcgctgga tgaacgacgc agggagacgc tggcccgtcg
     2161 gtgtcaccgt gctcacggtg ctggccgtct tctatacgct ctatttcgcc cgtgcattcc
     2221 tgatcccgat cactttcgcc gtgctgctcg acttcctcct cagtcccgcc gtccgggccc
     2281 tggtccgctg gcacgtgccg ctgccggtgg cggcggcgct cgtgctcttc gccatgctgg
     2341 gcacgggcag cctgttcgtc tacgacctct ccggcccggt gcagagctgg gcggacaacg
     2401 cgcccgagac gctggcgacg gcgcagacgc gcttgcgccg gctgctcaag ccgctgcagc
     2461 gggtgacgaa gaccgcggag caggtggaga gcgcggcgac cggcggggtc gcgggaggag
     2521 gggccgcgaa gccgaccgaa gtcgtggtgc gcggccccag catcgtgtcg cgcctgttcg
     2581 gctcgacgca gcggtttctc atcggcctgc tcgaggtcac gttcctgctg tatttcctgc
     2641 tcgccgcggg cgacctcttc ctgcagaagc tgatcaaggt gctgccgaac gtgcacgacc
     2701 ggcacaaggc gatcgagatc gcgcgggcca cggaggcgtc gatttccacc tacctcctca
     2761 ccaccctcgc cgtgaacgcg gtcgagggcg gcgtggtcgg ggccgcgctg tgggccctcg
     2821 gcatgccgaa tcccgtgttg tggggcgcgc tcgtcacgct gctcgagttc attccgtacc
     2881 tcggagcgct gagcctcgtc gtgctcctca ccctcggggc gttcgcgacc ttcaacgacg
     2941 tcgggcatgc gctgctgatt ccggccgcct ttctcgcgat caacctgatt caggcgaacc
     3001 tcgtcagccc gatgctgctg ggccaccggc tcgccctgaa ccccgtcgcc ctcctcgtca
     3061 gcctcgcctt ctggctgtgg atctggggca ttcccggagg gctgctcgcc gttcccctgc
     3121 tcgccaccct caagatcttc tgcgaccaca tcgagcggct cgccccggtg ggcgaattcc
     3181 tcggccggcg cgacggagag ctccagccgc cgatcctgaa gagcagttga ccgaagtccc
     3241 tttggcgctg cacaattgag catgcgcccg cgcctgctcg tgtcgatctg ctgcctcgcc
     3301 gcctgcgccc gctccggcgg cctctcccat cccgcgccga catcgacctg ggagctcgcc
     3361 ggcaaagcag tggcggtgac ggcgccccac gccatggtca ccaccggcca ccccctggcc
     3421 accgaggtcg gccgccgcgt cctggagcag ggcggcaacg ccatcgacgc cgccgtggcc
     3481 gtcggcttcg ccctgaccgc ggtgctcccc gcggccggga acatcggggg cggcgggttc
     3541 atcgtgtacc gcgacagcgc cggcgcggtg agcgccctcg attatcgcga ggccgccccg
     3601 gcggcggcga gccgggacat gttcgtcgat tcgacgggcg aaccgaccga gcgaagtatc
     3661 acgggtcacc tctcggccgg agtcccgggg agcgtcgccg gcctgtacga ggcctggagg
     3721 cggttcggga agctgccctg gccggccctg gtcgcgccgg cgatcgcgct ggccgagggc
     3781 catcccgtgg actcgtcacg cagcagcggg ctggcgggag actccgttcg tctcagcctg
     3841 tttcccgcct ccgcggccca gttcctcgtc gccggtcggg tgccccagcc gggcaccgtg
     3901 ctccatcaac ccgacctggc ccggacgtta cggctcatcg ccgattcggg tcccgacgtc
     3961 ttctatcgcg gccagatcgc cgacctgatc gtggcggaga tggcacgggg cggcggcatc
     4021 atcacccggg aagacctggc ggcgtatcgg cccaagtggc gcgagccgat tcgcgtggag
     4081 taccgcggct acgcggtcta ttcgatgccg ccgtcgagct cgggcggcgt gacgctggcg
     4141 gagatcctga acatcctcgg cgggtacgac tcccttccgc ccttcggcag cacggccctg
     4201 ctccacctgg agacggaggc gatgcggcgc gcgttcgagg accgcaacca ctggctgggc
     4261 gatcccgatt tcgtgcaaat gccgctcgac cgcctgctct ccaagagcta cgccgcggaa
     4321 ctgcgcgccc agatcgaccc cacccgggcg acccccacgc cgccgttcca ggcgggcccg
     4381 cccggcagca ccgagaccac ccactactcg atcgtggacg ccgacggcaa cgccgctgcc
     4441 gtgaccacca cgctcaacaa cagctacggg agcgcggtga ccgtcacggg agcggggttc
     4501 ctgctgaacg acgagatgga cgatttcgcg accgcgcccg gaaagcccaa catgttcggg
     4561 ctggtgcagg gcgaggtcaa cgccgtcgcc ccgggaaagc gcatgctctc ggcgatgacg
     4621 ccgaccatcg tcctcgaccc cgagggccgg ctcttcctcg tcgcgggcgc ccccggcggc
     4681 ccgcgcatca tcaccgcggt ctatcacgcc atcagcaacg tgatcgacca tcacatgagc
     4741 ctcgcccagg ccatcgaggc gccgcgcgga caccaccagg cgctcccgga cacgctcatg
     4801 gtcgagcggg gcgggttcaa cgtggcggtg ctcgattcct tgcgggcgat gggacacggc
     4861 gtgcgcgagt ggggctatca cgcctacgtg atgggcatcg agcgcggacc gcgcgggtgg
     4921 gtgggggtgg cggatccgag aagcggggga aacgcggcgg ggtactgaag ggcggaagaa
     4981 ggcggaagaa ggcggaaaag ggcggaaaag gacggaacgg gtccgccttt ttccgccctc
     5041 ctccgtcttc ttccgccttc ttccgtctag ctcaccttca ccgtcaccgc actcaccagc
     5101 gacgcgttga tgtgcgctcc cttggggagc acgatgtcgg cgtccttggt ctgcgtggca
     5161 acgccggcgc ccacggcacc gcccaccacg ccaccgatga tcgccccctt cgcgttcttc
     5221 cccaggaccc ggccgaggat cgccccggcc gccgcgccgg cgcccaccgt ggcgacgtcg
     5281 ccgcccgtga cgccgcgacc cgcccgcacc gtctccagcg agtcgatctt cgcgtcgaga
     5341 gggtagtccg tcccaccgac gctgacggac gtcagggaca gttgcagggt gccgggcgtg
     5401 ctgggattcg gcgccggctt cacggcggcc acgctgccgt gcacggtgga cccggcgggg
     5461 atgaccacgc ggccgtttgc gtccttcacg tcggccacga cggacgccgt gaacgcgtca
     5521 ccgaccttcg tggtccgcga ggagagcgta tcgctcaccg ccaagtcgag gaacgaaccc
     5581 gcggcagcgg tgcgcgtcgc agcggcggcg ggcgtgggtc tgggggccgg cggcttcgtc
     5641 ttgggcgccg ccgggcggga gggcggcggt gtgggcgcgg gttgggccgc gggtgcgggc
     5701 acgtcctgca tcgccgccgt ggactccgtc ggggcgagcg tgaggttccg cgccgccgag
     5761 tcggcccgct ggtccgccgt cttgtcggat ttcgcgcagg cgcccgccgc gacgaggccg
     5821 agcaccagcc ccagcgacag ggtccacgag gatttcgagt tggtaggcat cttcgggtct
     5881 ccaggtaccg ggaaagggga cgacagcctt cgtgcggcgg tcccctgccc tagaatgtcg
     5941 cggggcggcg cgcctgactt caagcccttg ccgcgccgtt cacttgacct cgaaggcctt
     6001 ctggccgacg gattggccgt tgagccagat ggccagggtg tatccccccg ccggccaggg
     6061 cgacgccttc atgatgtgga actccgtctg ggccgggccg gtcggcgcga tcgtcatcgt
     6121 ggtggaatcg accgtctgac cggtctggta ggtccacttg gcggtgagca ccgcgctggt
     6181 ggccgtgccg gtggtcgcga cggacgcgta gatcgtgtcg ttcacaccga aggtcgtcat
     6241 cgactgcacg acgcggttgt ccgggccaat ctggcggccg aggtcgaccg ctgcgacttc
     6301 gaccggggcc tgggccggcg gcggtgcggt cttcttgcag gcggcgagga cgctgagtcc
     6361 cagaaccgcc gcggcggccc ggtgcaggct gtggtggtac atgcgtgtcg actccttcag
     6421 ggttagacct gaggaatccg gaacacctgt cccggataga tgaggtcggg gttcttgatg
     6481 cttaccttat tggcctcgta gatcaccgtc cattgcttcg cactgccgta cagcgccttg
     6541 gcgaccttcg acaggctgtc ccccttttgg accgtgtagg tccgctcctg gggctgcgg
//
LOCUS       DBNGZW010000336         4221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_642537, whole genome shotgun sequence.
ACCESSION   DBNGZW010000336 DBNGZW010000000
VERSION     DBNGZW010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4221
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_642537"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="AC16JC_07965"
     CDS             <1..701
                     /locus_tag="AC16JC_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013513219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HIR5164667.1"
                     /translation="MNFAGLPERLALIRAEIARRQAAGGWTHPVTIVAVTKGFGPDAV
                     VAARAAGLPDVGENRVQEALDKMAAVPDTGVVWHLSGHLQRNKARLVPGRFALVHSVD
                     SLSLAEELAHRAAGQGVRQRVMVQVNVAGEEQKSGCPLADAPALLRGVAGLEALALEG
                     LMAMAPLTDDAEVQRTTFRGLRRLRDAMKEEGLWVPTLSMGMSGDFATAVEEGATVIR
                     LGTALFGARTTGTA"
     gene            698..1168
                     /locus_tag="AC16JC_07970"
     CDS             698..1168
                     /locus_tag="AC16JC_07970"
                     /inference="COORDINATES: protein motif:HMM:NF016959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIR5164668.1"
                     /translation="MTDDVFHLTPLDVRRQEFRRSLRGYEPLGVEDFRMRVADELERV
                     LREKSVLEERVAALTEQLRVFRERETAMNEALVAAQQLREETRAAAEREAQVILREAE
                     AEGRRQIDGAERAEQDAHLRMAEVQRQFAGYLAGFRALLDRQLAELRALEGDRG"
     gene            1165..1974
                     /locus_tag="AC16JC_07975"
     CDS             1165..1974
                     /locus_tag="AC16JC_07975"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018128143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR5164669.1"
                     /translation="MTVAETVQAVRARTGLVPEVAIVLGTGLGALTELLDADAVIPYA
                     ELPGFAVPTVESHAGRLHVGTLAGRRVAMLQGRFHRYEGYALDQVTFPVRVLHALGAG
                     TLIVSNVSGGMHPLWEAGDLVLLADHINLLGDNPLAGPDDGGGSARFPDLTEAYDPDL
                     RALARDVALEQGITLREGTYVAVAGPNLETRAEYRMLRMLGGDVVGMSTVPEVIVARQ
                     LGMRVLGLSIITDLCLPDALEPASVEQILAVARVAEPRLAALVQGLIPRLH"
     gene            1974..>4221
                     /gene="ileS"
                     /locus_tag="AC16JC_07980"
     CDS             1974..>4221
                     /gene="ileS"
                     /locus_tag="AC16JC_07980"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR5164670.1"
                     /translation="MAYPPLPADPSADALEKQLLATWKHERLFARVQEAARYGPPFVF
                     FEGPPTANGRPGIHHVFARTIKDLVCRYHTMLGQGVTRIAGWDTHGLPVEIEVERQLQ
                     ISGKKQIEAFGVERFNQMCRESVFTYKTDWEALSDRIAYWLDYEHPYITYTKDYIETV
                     WWLLRRLHDRGYLYRGHKVLPYCPRCGTALSSHELALGYDQEQTNSVYVTFPLADDPK
                     GQLVVWTTTPWTLVSNVAVAVHPDLEYGEYALPDAHGEVRRYLAAAARAGDIHVGGRT
                     LAEGELVRTFRGRDLVGQAYRRPLDVVPLPPDGKREVVVAADFVSAEEGSGLVHMAPA
                     FGADDYATGQREGLAFVNPVAPDGTFQGTSWPEIEGKLVTDKATNHTIIERLKHDGRW
                     LETRPLTHTYPFCWRCDSPLIYYARSSWFVRTTAYKQRLLEANAGVQWHPPEIGTGRF
                     GEWLANNVDWAISRDRYWGTPLNVWECEREREHREVIGSYEELAARSGRPIPADFDPH
                     KPFIDAVTWTCRSCAGTMRRVPEVIDAWFDSGAMLAAQWHYPFAHEAEFKAHFPADFI
                     CEGVDQTRGWFYSLLAIAVGCFDAPAYRNVIVGELVLDAQGQKMSKSRGNIVNPWQVI
                     ERHGADAVRLYLLTQSQVWLPKRFNTEDVGKGGFLNTLRNTYQFFQMFAGDWTPQVPV
                     PAPAERPLVDRWLLARLDQTIAAVRTAWNDYDVTTGVRAIIDFVVEDLSTWYVRLNRP
                     RFWAPDRAA"
BASE COUNT          619 a         1409 c         1564 g          629 t
ORIGIN      
        1 ctatgaattt tgccggtctg ccggagcggc tcgccctcat tcgggccgag atcgctcggc
       61 ggcaggcggc aggcggatgg acccatcccg tcacgatcgt ggcggtgacc aaggggttcg
      121 ggcccgatgc agtcgtcgcg gcccgggcgg ccggcctccc cgacgtgggg gagaaccggg
      181 tgcaagaggc gctggacaag atggccgcgg tgcccgatac gggcgtggtg tggcacctca
      241 gcggtcatct gcagcgcaac aaggccaggc tcgtccccgg ccgcttcgcg ctggtgcact
      301 cggtggattc gctctcgctc gccgaggagc tggcgcaccg ggcggcaggg cagggcgtgc
      361 gccagcgggt gatggtgcag gtgaacgtgg cgggggaaga gcagaagagc ggctgcccgc
      421 tggccgacgc tcccgcgttg ctgcggggcg tggcggggct ggaggcgctg gcgctcgagg
      481 gtctgatggc catggcgccg ctgaccgacg acgcggaggt gcagcgaacg accttccgcg
      541 gactgcggcg gttgcgcgat gcaatgaagg aggagggact atgggtgccg acattgtcga
      601 tggggatgtc cggcgacttc gctacggcag tcgaggaggg ggcaacggtg atccgattgg
      661 gtaccgccct gttcggagcg aggacgacgg gaacggcgtg accgacgacg tcttccatct
      721 gacgccgctc gacgtgcggc ggcaggagtt ccgccgcagc ctccgcggct acgagccgct
      781 gggcgtcgaa gacttccgca tgcgggtggc ggacgagctg gagcgcgtgc tgcgggagaa
      841 gtcggtgctg gaggagcgcg tggcggcgct gaccgagcag ctgcgggtgt tccgcgagcg
      901 cgagacggcg atgaacgagg cgctggtggc ggcgcagcag ctgcgggagg aaacgcgagc
      961 cgcggccgag cgcgaggcgc aggtgatcct ccgcgaggcg gaggccgagg ggcggcggca
     1021 gatcgacggg gcggagcggg ccgagcagga tgcgcatctc cggatggcgg aggtgcagcg
     1081 gcagttcgcc gggtacctgg cggggttccg cgcgttgctc gaccggcagc tggccgagct
     1141 gcgcgcgctg gagggcgatc gcgggtgaca gtggcagaga cggtgcaggc ggtccgcgcc
     1201 cgcaccggcc tcgtgccgga ggtggcgatc gttctgggca cgggcctcgg cgccctgacc
     1261 gaattgctcg atgccgacgc cgtgatcccg tatgccgagc tccccgggtt cgcggtcccg
     1321 accgtggaga gtcacgccgg gcgcctccac gtgggcacgc tcgcggggcg tcgcgtcgcg
     1381 atgctgcagg gccggttcca tcggtacgag ggctacgccc tcgatcaggt gaccttcccg
     1441 gtgcgcgtgc tgcacgcact cggcgccggc acgctgatcg tctcgaacgt gagcggcggg
     1501 atgcatcccc tgtgggaggc cggcgatctc gtgctgctcg ccgatcacat caatctgctg
     1561 ggtgacaacc ccctcgccgg tcccgacgac ggcggcgggt ccgcgcgttt ccccgatctc
     1621 accgaggcgt acgacccgga cctgcgtgcg ctcgcccgcg acgtcgcgct cgagcagggc
     1681 atcaccctgc gggaagggac ctacgtcgcg gtggcggggc ccaatctcga gacccgcgcc
     1741 gaataccgca tgctgcgcat gctggggggc gacgtcgtcg ggatgagcac ggtgcccgag
     1801 gtgatcgtcg cgcgccagct ggggatgcgg gtgctgggcc tgtccatcat caccgacctg
     1861 tgcctgccgg acgcgctcga gcccgcctcg gtggagcaga tcctggcggt ggcccgcgtg
     1921 gccgagccgc ggctcgcggc gctggtgcag ggccttatcc cgcgcctgca ctgatggcct
     1981 atccgccgct gccggccgac ccgagcgccg acgcgctcga gaagcagctg ctcgcgacct
     2041 ggaagcacga gcggctgttc gcgcgcgtcc aggaagcggc gcgctacggt ccgccgttcg
     2101 tgttcttcga aggcccgccc accgccaacg gccgccccgg gatccatcac gtcttcgcgc
     2161 gcaccatcaa ggacctcgtg tgccgctacc acacgatgct gggccagggc gtgacgcgca
     2221 tcgcgggctg ggacacgcac gggctcccgg tggagatcga ggtcgagcgc cagctccaga
     2281 tcagcggcaa gaagcagatc gaggccttcg gcgtcgagcg cttcaaccag atgtgccgcg
     2341 agagcgtgtt cacgtacaag accgactggg aggcgctctc cgaccggatc gcctactggc
     2401 tggactacga gcatccctac atcacctaca ccaaggacta catcgagacg gtgtggtggc
     2461 tgctgcgccg gctgcacgac cgcggctatc tgtatcgcgg gcacaaggtg ctgccgtact
     2521 gcccgcgctg cggcacggcg ctgtcctccc acgagctggc gctgggctac gaccaggagc
     2581 agaccaactc ggtgtacgtc accttcccgc tcgccgacga cccgaagggc cagctcgtcg
     2641 tgtggaccac cacgccctgg accctcgttt ccaacgtcgc cgtggcggtg catccggatc
     2701 tcgagtacgg ggaatatgcc ctgcccgacg cccacggcga ggtccgtcgc tacctcgccg
     2761 ctgccgcgcg cgccggcgac atccacgtgg ggggcaggac cctcgccgag ggggagctgg
     2821 tgcgcacttt ccgcgggcgc gacctggtcg gccaggcgta ccggcggccg ctcgacgtag
     2881 tgccgctgcc gccggacggc aaacgcgagg tggtcgtcgc cgcggatttc gtctcggccg
     2941 aagagggttc gggcctggtg cacatggcgc ccgcgttcgg cgcggacgac tacgccaccg
     3001 ggcagcgcga ggggctcgcg ttcgtgaacc cggtcgctcc cgacgggacg ttccagggga
     3061 cgtcgtggcc ggagatcgag gggaaactgg tcaccgacaa ggcgaccaac cacacgatca
     3121 tcgagcggct caagcacgac ggccgctggc tcgagacgcg cccgctgacg cacacctatc
     3181 cgttctgctg gcggtgcgat tcgccgctca tctactacgc gcgcagctcg tggttcgtcc
     3241 ggaccacggc ctacaagcag cggctgctcg aggcgaacgc cggtgtgcag tggcacccgc
     3301 ccgagatcgg gacgggccgc ttcggcgaat ggctcgcgaa caacgtggac tgggcgatct
     3361 cgcgcgaccg ctactggggc acgccgctca acgtgtggga gtgcgagcgc gagcgggagc
     3421 atcgcgaggt catcggctcc tacgaggagc tggccgcccg ctcggggcgc cccatcccgg
     3481 ccgacttcga tccgcacaag ccgttcatcg atgcggtgac ctggacgtgc cgcagctgcg
     3541 ccgggacgat gcggcgggtg cccgaggtga tcgacgcctg gttcgattcg ggcgccatgc
     3601 tcgccgcgca atggcactac ccgttcgccc acgaagcgga attcaaggcg catttccccg
     3661 cggacttcat ctgcgagggg gtggaccaga cccgcggctg gttttactcg ctgctggcga
     3721 tcgccgtcgg gtgcttcgac gcgcccgcct accgcaacgt gatcgtgggc gagctggtgc
     3781 tcgacgcgca ggggcagaag atgtccaaga gccgcggcaa catcgtcaac ccgtggcagg
     3841 tgatcgagcg ccacggggcg gacgcggtgc ggctctacct cctgacccag agtcaggtgt
     3901 ggctgcccaa gcggttcaat acggaggacg tggggaaggg cggcttcctc aatacgctgc
     3961 gcaacacgta ccagttcttc cagatgttcg cgggcgattg gacgccgcag gtgccggtcc
     4021 cggcccccgc cgagcgtccg ctcgtggatc gctggctcct ggcccggctc gaccagacca
     4081 tcgcggccgt gcgcaccgcc tggaacgact acgacgtgac gaccggcgtc cgtgcgatca
     4141 tcgatttcgt ggtggaggat ctgtccacgt ggtacgtgcg gctcaaccgc ccccgcttct
     4201 gggcccccga ccgggcggcc g
//
LOCUS       DBNGZW010000337         2978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1256848, whole genome shotgun sequence.
ACCESSION   DBNGZW010000337 DBNGZW010000000
VERSION     DBNGZW010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1256848"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            347..703
                     /locus_tag="AC16JC_07985"
     CDS             347..703
                     /locus_tag="AC16JC_07985"
                     /inference="COORDINATES: protein motif:HMM:NF017451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5164671.1"
                     /translation="MVHHVAIEVAADVVRVAQTFRGAGAFSRGEQMVRSALSVSSNIA
                     EACGRGTQREFRHYIAIARGSAQELLSQLRVSSLIQTSPDGLRILRSIQNRTVLVIKM
                     LSRLDASPPPLREPGA"
     gene            complement(831..1094)
                     /locus_tag="AC16JC_07990"
     CDS             complement(831..1094)
                     /locus_tag="AC16JC_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164672.1"
                     /translation="MSEPFPGQERARHMGELKRGPSRWDVYLEVEPDPELQAMRGRIH
                     FVGEGARKTTGWVFLERQEKEILERFGEFSAVELLQFVESIPG"
     gene            1148..1828
                     /locus_tag="AC16JC_07995"
     CDS             1148..1828
                     /locus_tag="AC16JC_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR5164673.1"
                     /translation="MNCHACGATLSSGARFCHKCGANTNPTGASTGWKAGLPWGIAGL
                     AVGALLVVVLMRAGGPAAATGTPPVGAAPVAGGGASALDISQMSPEERAQRLFDRVMR
                     LQEEGKQDSVQFFLPMAIGTYQQLPAMTLDSHFDIGLLQLVGGDAAAALAEADTIRRQ
                     APTHLFTYVLRARAAQATNDTRAVTQAYRDFLKNETAERARKRPEYDEHARILDGFHA
                     EATSRSGS"
     gene            1788..2681
                     /locus_tag="AC16JC_08000"
     CDS             1788..2681
                     /locus_tag="AC16JC_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis family protein"
                     /protein_id="HIR5164674.1"
                     /translation="MGSTPRPPAGPGADRRLTRLIHVAHSPDSDDAFMFWALAAGKID
                     TGDRRYVHELADIETLNRRALAGELDVTAVSLHAYAYLADRYVLLAHGASIGDRYGPR
                     IVARAAPSADPRVALAGRTVAVPGELTTAYLALRLYQPDARHVVVPFDQIEDYVAAGR
                     ADAGLLIHEGQLTFADRGLHLWVDLGEWWHAETGLPLPLGGNVARRDLGEPLLAAIAR
                     DLKASIEYGLAHRAAALAHAQSYSRGLDPARTDRFVGMYVNDFTVEYGERGRRAVEEL
                     LARGHRAGLLPAPVALQFIGA"
     gene            2714..>2978
                     /locus_tag="AC16JC_08005"
     CDS             2714..>2978
                     /locus_tag="AC16JC_08005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5164675.1"
                     /translation="MPNRPPHPSVALLVVDDEEPIRNALKRFLEGEQFAVHTAASGQE
                     ALTVLQQHDIALLLADIRMPGMSGIDLVPQALEVDPGLAVVVLS"
BASE COUNT          463 a         1063 c          939 g          513 t
ORIGIN      
        1 acggcaacag cgtcaagcag aggcgtcgag cgatggcgtc aagcagattc gtcaagcgga
       61 ctcgtctcgg cacgcgcttt tccgtatggc tcaggcatga gaatgatgct ggaaggcggc
      121 cgccggcagt ccatcgtatc tggtgagtct tcgcgccgat ccgagtaagc gaatacggca
      181 acagcgtcaa gcagaggcgt cgagcgatgg cgtcaagcag attcgtcaag cggactcgtc
      241 tcggcacgcg cttttccgta tggctcaggc atgagaatga tgctggaaga cggccgccgg
      301 cagtccatcg tatctggtga gtcttcgcgc cgatccgagt aagctaatgg tgcaccacgt
      361 cgccatcgag gtggcagcgg acgtcgtgcg cgtggctcag accttccgtg gagccggtgc
      421 gttctcccgt ggtgagcaga tggtgaggtc ggcgctctcg gtgtcatcga acatcgctga
      481 agcctgcggt cgcggcacgc aacgcgaatt ccgtcactac atcgccatcg cccgtggttc
      541 tgcccaggaa ctcttgtcgc agctccgtgt gagcagcctg atacagactt ctccggacgg
      601 gctgcgcatc ctcaggtcca tccagaatcg gacggttctc gtcatcaaga tgctcagccg
      661 cctcgatgcg agccctccgc cactacgcga acccggcgca tgagcgatac gcctttgctt
      721 ttgacgcctc tgcttgacgc ctctgcttga cgcctctgct tgacgcctct gcttgacgcc
      781 tctgcttgac gcctttgctc gacgcctttg ctcgacgcct tcgccttttt ctatcccgga
      841 atcgactcga caaactgcag caactccacg gccgaaaatt caccgaatcg ctcgaggatt
      901 tccttttcct ggcgctcgag gaacacccag ccggtcgtct tgcgagcccc ttcgcccacg
      961 aagtggatgc gaccgcgcat cgcctgcagc tccgggtcgg gctccacctc gagatagaca
     1021 tcccagcggc tcggcccgcg cttcagctcg cccatgtgcc gcgctcgctc ctgcccgggg
     1081 aatggctcgc tcattcccca tacgctacgc tgccatcgtc gcggatgcca gttagattcc
     1141 tgcccctatg aactgtcacg cctgcggtgc gacgctctct tccggcgccc gtttctgtca
     1201 caagtgcgga gccaacacca atccaaccgg cgcgtctacg ggctggaagg cgggcctccc
     1261 ctggggcatc gccggccttg cggtcggcgc cctcctcgtc gtggtcctga tgcgcgccgg
     1321 cgggccggcg gcggccaccg gcacgccccc cgtcggtgca gctcccgtcg ccgggggtgg
     1381 cgcctccgcc ctggacatca gccagatgtc gcccgaggag cgcgcgcagc gcctgttcga
     1441 ccgggtgatg cggctccagg aagagggcaa gcaggacagc gtgcagttct tcctgcccat
     1501 ggcgatcggg acctaccagc agcttcccgc catgaccctc gactcgcact tcgacatcgg
     1561 gctcctgcag ctggtgggag gcgacgcggc ggcggcgttg gccgaggccg atacgattcg
     1621 ccggcaggca ccgacccact tgttcaccta cgtgctgcgc gcccgcgcgg cccaggccac
     1681 gaacgacacc cgtgcggtga cccaggccta tcgggacttc ttgaagaacg agaccgccga
     1741 gcgcgcccgc aagcggccgg agtacgacga gcacgcccgc atcctggatg ggttccacgc
     1801 cgaggccacc agccggtccg ggagctgacc ggcgcttgac ccgcctcatt cacgtcgcgc
     1861 atagcccgga ttccgacgat gccttcatgt tctgggcgct cgcggccggg aagatcgaca
     1921 cgggcgaccg ccgctacgtc cacgagctcg ccgacatcga gacgctgaac cggcgcgccc
     1981 tcgccggcga gctcgacgtc accgcggtat cgctccacgc ctacgcctac ctcgccgatc
     2041 gctacgtcct gctggcccac ggcgcctcga tcggcgaccg ctacggcccg cgcatcgtgg
     2101 cgcgcgcggc cccttccgcg gatccgcgcg tggccctcgc tgggcggacc gtcgctgtcc
     2161 ccggggagct caccaccgcc taccttgcgc tgcggctgta ccagcccgac gcccggcacg
     2221 tcgtcgtccc gttcgaccag atcgaggatt atgtcgccgc gggccgcgcc gacgccggcc
     2281 tgctcatcca cgaaggacag ctcacgttcg ccgatcgcgg gctgcacctc tgggtggatc
     2341 tgggcgaatg gtggcatgcg gagaccggat tgcccctgcc gctgggcggc aacgtcgcgc
     2401 gacgcgacct gggagagccg ctgctcgcgg cgatcgcccg cgacctcaag gccagtatcg
     2461 agtacgggct cgcgcaccgt gccgcggccc tggcgcacgc gcagagctac agccgggggc
     2521 tcgatcccgc ccggaccgac cggttcgtgg gcatgtacgt gaacgacttc accgtggagt
     2581 acggcgagcg gggacgccgg gccgtggagg agctgctggc gcgcggccac cgggcgggcc
     2641 tcttgccggc tcccgtggcg ttgcagttca tcggcgcgta aacttcgatt cctcaccaga
     2701 aagatcgaat cgcgtgccga atcgcccgcc acatccctcc gtcgccctgc tggtcgtgga
     2761 cgacgaggag ccgattcgga acgccctcaa acgattcctc gagggggagc aattcgccgt
     2821 acacacggcg gcctccggcc aggaggcgct caccgtgctc caacaacacg acatcgccct
     2881 tctgctggcc gacatccgga tgcccggcat gagcggcatc gatctcgtgc cgcaggcgct
     2941 cgaggtcgac cccggcctag cggtcgtggt gctctcgg
//
LOCUS       DBNGZW010000338         4774 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1794212, whole genome shotgun sequence.
ACCESSION   DBNGZW010000338 DBNGZW010000000
VERSION     DBNGZW010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4774)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4774)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4774
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1794212"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="AC16JC_08010"
     CDS             <1..183
                     /locus_tag="AC16JC_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164676.1"
                     /translation="MLDVGKLTGYLWDKHRIIVTAIAHPEFNCIRVTPNVYTLAAEVD
                     RFADVVEETMKQGIPA"
     gene            180..407
                     /locus_tag="AC16JC_08015"
     CDS             180..407
                     /locus_tag="AC16JC_08015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164677.1"
                     /translation="MKSLPAIGYQLSVLLLVACGSGGGSQRQAGDTLTERQRDSMLSQ
                     SRIPGASGVGKAMNAADSASARVRAADTIGP"
     gene            421..1023
                     /locus_tag="AC16JC_08020"
     CDS             421..1023
                     /locus_tag="AC16JC_08020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR5164678.1"
                     /translation="MLGIYIVAAILGAGLLALSLLAGGDGGHDVAGDLGGHALGADHP
                     MLADAEHPGFGEAVLSLFRPRNFIFLLAGFGLSGTLLTLIHTGPVFTLVAALGMGGGF
                     FLLSHLVFTLLRRSDTAGGALTEVELMGERARVTLPLVPGHPGRVACLLGGREVYLTA
                     RLAPGITDTIAAGREVIVNSVTNGVAEVVPPERYDRQLLP"
     gene            1038..2327
                     /locus_tag="AC16JC_08025"
     CDS             1038..2327
                     /locus_tag="AC16JC_08025"
                     /inference="COORDINATES: protein motif:HMM:NF013324.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0016600 - flotillin complex [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HIR5164679.1"
                     /translation="MDSFPAAYIVLGAIALGLVTALVAMVRSLIVICLPNEMVVVTGR
                     RRTLEDGREVGYRVLHGGRTLRIPVIEKVSRIDLRTIPIEVVVKNAYSKGGIPLVVQG
                     IANVKVCAREPVSYNAVERLLDKPFDEIMQIAKDTLEGNLRGVLATLTPEEVNEDRLK
                     FAGTLIEEADEDLKKLGLELDTLKIQNVTDEVNYLDSIGRQRTAEIQKLARVAEATRA
                     SEAEQTEAAAHQAAQVAKIQAELKITEENNRLRVRTAELNGTALASEAEAQVAGEKAK
                     VKAQQDLQAERIVLEGKRVEADVVVPARADKEASELRAKGAAASILENGNANLSVLDR
                     MIKLYGQAGADAERIFILNMLPDLIRELAGSVEKIKIDKLSVIDSSRGSGVASAAAQF
                     PEAIIKLTEQIETATGVNILSRLQRPVPQMESPAKQA"
     gene            2387..2755
                     /locus_tag="AC16JC_08030"
     CDS             2387..2755
                     /locus_tag="AC16JC_08030"
                     /inference="COORDINATES: protein motif:HMM:NF013561.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HIR5164680.1"
                     /translation="MSKLTRVTVTLPSDLLAAADARARQLDRSRSRVVAEALRTYLAA
                     RVVREPGVAYAGREVAEARRLHLATELRLPAAERLRRAEELGRLGRAAQRRRPRQQII
                     GFDTYEDYYEWKRKAHLVGV"
     gene            2752..3207
                     /locus_tag="AC16JC_08035"
     CDS             2752..3207
                     /locus_tag="AC16JC_08035"
                     /inference="COORDINATES: protein motif:HMM:NF022167.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR5164681.1"
                     /translation="MTPKAIATQVCEALNRSGARYLVIGGIACVLHGYVRATTDVDIL
                     IERTLANAERVLEALATVGYGFAREWSAAEILKRPITVIGDDPAVDVFTVAWKVKYEQ
                     AAKRSSVVEVEGVSIPLIAIPDLIETKRTGRALDAADIEALEEIERLRG"
     gene            complement(3231..4748)
                     /locus_tag="AC16JC_08040"
     CDS             complement(3231..4748)
                     /locus_tag="AC16JC_08040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018718932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional activator/DNA
                     repair enzyme AdaA"
                     /protein_id="HIR5164682.1"
                     /translation="MRLTSDVCQRALRARDARFDGIFFVGITTTRVYCRPVCPSRLAF
                     PARRRFFDSAAAAELAGFRPCLRCRPELAPGRAICDAVPRLARVAAHRIAAGALNGHS
                     VAQLARELGVSERHVRRALEREIGVSPVELAQTHRLLLAKRLLADTNLPVTRIAFASG
                     FQSLRRFNSVFRERYRLSPSALRPRPRASRPENDLVRLTLAYRPPLAWDVLLEILGRG
                     ALPGIEVVRDGRYGRTVCLDGRSGVVFAANAAAEPRRGHGAPRTHLNVDVSPALLPVL
                     MPLLSRLRHLFDLDAEPTVIDAHLAESGLGALIERRRGVRIPGAVDGFECALGVLLGG
                     SGSARRAVVRTLGERLDTGIPELRYLAPGAARIAGAGVARLASLGLGPRRARALVAVA
                     RAVVAGTLCLEPGSDLGTARRALLGIDGIGDRDATTIVMRALYWPDAFAASDPALRRA
                     AGTVSARELYIRAERWRPWRAYAALHLWLSRSAVPPGTSRRPGSSPWRAVADLPA"
BASE COUNT          771 a         1593 c         1779 g          631 t
ORIGIN      
        1 atgctcgacg tgggcaagct gaccggctac ctgtgggaca agcaccgcat catcgtgacg
       61 gcgatcgcgc accccgagtt caactgcatc cgggtgacgc cgaacgtgta cacgctggcg
      121 gcggaggtgg accggttcgc ggacgtggtg gaggagacga tgaagcaggg gattccggca
      181 tgaagtcgct accggctatt ggctatcagc tatcggtcct gctgctcgtg gcgtgcggga
      241 gcggaggcgg gagtcagcgt caggcaggtg acacgctgac cgagcggcag cgcgacagca
      301 tgctgtcgca gtcgcgcatt cccggcgcgt cgggggtggg caaggcgatg aacgcggccg
      361 actcggcgag cgcccgggtg cgggcggcgg atacgatcgg cccgtaaggt cgggggggcg
      421 atgctcggga tctacatcgt cgcggcgatc ctgggcgccg ggctgctggc gttgagcctg
      481 ctggccggcg gcgacggggg acacgacgtc gcgggagacc tcggaggcca cgccctcggg
      541 gcggaccacc cgatgctcgc cgatgcggag catcccggct tcggcgaggc ggtgctcagc
      601 ctgtttcgtc cccgcaactt catctttctt ctggcgggat tcgggctttc cggcacgctg
      661 ttgaccctga tccacacggg gcccgtgttc acgctggtgg cggcgctggg catggggggc
      721 gggttcttcc tgctgagcca cttggtgttc acgctgctgc gccggtccga taccgccggc
      781 ggggcgctca ccgaagtgga actgatgggc gagcgggcgc gcgtgacgct acccctcgtg
      841 ccgggccatc caggccgggt cgcctgcctg ctgggcgggc gcgaagtcta tctgaccgcc
      901 cggctcgccc cggggatcac ggacacgatc gccgcgggac gcgaagtgat cgtgaacagc
      961 gtcacgaacg gcgtggcgga ggtggtgccc cccgagcgct acgatcgtca actcctaccg
     1021 taaggaccta cttcgccatg gacagctttc ctgccgccta catcgtgctg ggtgcgatcg
     1081 cgctcggcct cgtcaccgcc ctggtggcga tggtgcgctc gctcatcgtc atctgcctgc
     1141 cgaacgagat ggtcgtggtg accggccggc gccggacgct ggaggacggg cgcgaggtgg
     1201 ggtaccgggt gctgcacggc ggccgcacgc tgcgcatccc cgtgatcgag aaggtgtcgc
     1261 gcatcgacct gcgcaccatc ccgatcgagg tcgtggtaaa gaacgcctac tccaagggcg
     1321 gcatcccgct cgtcgtccag ggcatcgcca acgtcaaggt ctgcgcccgg gagccggtct
     1381 cctacaacgc cgtcgagcgc ctgctcgaca agccgttcga cgagatcatg caaatcgcca
     1441 aggacacgct cgaaggcaac ctgcgcggcg tgctggccac gctcaccccg gaagaagtga
     1501 acgaggaccg gctcaaattc gcgggcacgc tgatcgagga ggcggacgag gatctcaaga
     1561 agctcgggct ggagctcgac acgctgaaga tccagaacgt caccgacgag gtgaactacc
     1621 tcgactcgat cgggcggcag cgcacggcgg agatccagaa actggcccgg gtggcggagg
     1681 cgacgcgcgc gtccgaggcc gagcagaccg aagccgcggc gcaccaggcg gcgcaggtgg
     1741 cgaagatcca ggccgagctg aagatcaccg aggagaacaa ccggctgcgg gtgcgcacgg
     1801 cggagctcaa tggaacggcc ctggcgagcg aagccgaggc gcaggtggcg ggcgaaaagg
     1861 cgaaggtgaa ggcgcagcag gacctgcagg ccgagcgcat cgtgctggag ggcaagcggg
     1921 tggaggccga cgtcgtcgta ccggcacggg ccgacaagga ggcgtccgag ctgcgcgcca
     1981 agggcgccgc ggcatccatc ctcgagaacg gcaacgcgaa cctctccgtg ttggatcgca
     2041 tgatcaagct gtacggccag gcgggggcgg acgcggagcg gatcttcatc ctcaacatgc
     2101 tcccggatct gattcgcgag ctggcgggct cggtcgagaa gatcaagatc gacaagctct
     2161 cggtgatcga ttcgagccgg ggctcaggag tggcgtcggc cgcggcgcag tttcccgagg
     2221 cgatcatcaa gctcacggag cagatcgaga ccgcgaccgg cgtgaacatc ctgtcgcggc
     2281 tgcagcggcc cgtgccgcag atggagagtc ccgcgaagca ggcgtaggcg cgccgcgggt
     2341 cgtccccggg gctggcgatg gtcgctcata ccgggtatga ttcggtatga gcaagctcac
     2401 gcgggtcacc gtcacccttc cctccgatct gctcgcggcg gccgatgccc gggcgcggca
     2461 gttggaccgt tcccgcagcc gggtggtggc ggaggcgttg cggacctacc tcgcggcgcg
     2521 ggtcgtccga gagccgggcg tcgcgtatgc cggccgcgaa gtcgcagaag ccaggcgcct
     2581 gcacctcgcg acggagctgc ggctccccgc agccgagcgg ttgcggcgag ccgaggagct
     2641 ggggcgcctc ggacgcgccg ctcaacgccg caggccgagg cagcagatca tcgggttcga
     2701 cacgtatgag gactactacg aatggaagcg gaaggcgcac ctcgtcgggg tgtgaccccg
     2761 aaggcaatcg ccacgcaggt gtgcgaggcg ctgaaccgat ccggggcgag gtacctcgtg
     2821 atcgggggaa tcgcgtgtgt cctgcacggc tacgtgcgcg cgacgaccga tgtggacatc
     2881 ctgatcgagc gcacgcttgc gaacgcggag cgggtactcg aggcgctggc caccgtcggg
     2941 tacgggttcg cgcgagagtg gagcgcggcg gagatcctca agcgaccgat cacggtcatc
     3001 ggcgacgatc ctgcggtgga cgtcttcacg gtcgcctgga aggtgaaata cgagcaggcg
     3061 gcgaagcggt cgtccgtggt cgaggtggag ggcgtgtcga ttccgctgat cgcgatcccg
     3121 gatctgatcg agaccaagcg gacgggccgg gcgctcgacg cggcggacat cgaggcgctg
     3181 gaggagatcg agcggctccg cggctgagcc tccgctcccc cgggaccgga ttacgccggc
     3241 aagtcagcca ccgctctcca tggcgacgat cctgggcgcc gggaagtccc ggggggcacg
     3301 gccgaccggc tcagccacag atgcagggcc gcgtaggcac gccacggccg ccaccgctcg
     3361 gcacggatgt agagctcgcg cgcgctcacc gtcccggcgg cgcgccgtag cgccggatcc
     3421 gaggcggcga aggcatccgg ccagtagagc gcgcgcatca cgatagtcgt ggcgtcgcgg
     3481 tcgccgatgc cgtcgatgcc gagcaacgcc cgtcgcgccg tgccgaggtc gcttcccggc
     3541 tccaggcaca gggtccctgc gacgacggcg cgcgccaccg ccaccagggc gcgcgcgcgc
     3601 cgcggcccta gtccgagcga cgcgagacgc gccacgcccg cgccggcgat gcgcgccgcc
     3661 cccggagcga ggtagcggag ctccgggatc cccgtgtcca gacgctcccc cagggtccgc
     3721 accaccgcgc ggcgggcgga tcccgagccc cccagcagga cgcccaacgc gcactcgaag
     3781 ccgtcgacgg ctccgggaat gcgcacgccg cgccgccgct cgatgagcgc accgagcccg
     3841 ctctcggcca gatgggcgtc gatcaccgtg ggctcggcgt cgagatcgaa gaggtgtcgc
     3901 agccgcgaca ggagcggcat gaggaccggc agcagcgcgg gcgacacgtc gacgttcagg
     3961 tgcgttctgg gggcgccgtg cccgcgacgc ggctcggccg ccgcattggc cgcgaacacg
     4021 accccgctcc gtccgtcgag acacaccgtc ctcccgtagc ggccgtcccg caccacttcg
     4081 ataccgggca gcgcgccgcg acccaggatc tccagcagca cgtcccaggc gagcgggggg
     4141 cgatacgcga gcgtcaagcg caccaggtcg ttctcggggc gggaagcacg cggccgcggc
     4201 cgcagcgcgc tcgggctcag ccggtagcgc tcccggaaca cggagttgaa gcggcgcagg
     4261 ctctggaagc cgctcgcgaa ggcgatgcgc gtcacgggca ggttcgtgtc ggcgagcagt
     4321 cgcttggcga gcagcaggcg atgcgtctgg gccagctcga cgggggacac cccgatctcg
     4381 cgctccagtg cgcgacggac gtgacgctcg ctcacgccca gctcgcgcgc cagctgcgcc
     4441 acgctgtgcc cgttgagggc gcccgccgcg atgcggtgcg cggccacgcg ggcgagccgc
     4501 ggcaccgcgt cgcagatcgc ccggcccggc gcgagctcgg ggcggcagcg caggcaggga
     4561 cgaaatcccg ccagctcggc tgccgcggcg gaatcgaaga agcggcgccg cgcgggaaag
     4621 gcgagccgcg acgggcagac cggccggcag tacacccgcg tcgtggtgat gccgacgaag
     4681 aagatgccgt cgaagcgcgc gtcgcgcgcg cgcagggcgc gctggcagac gtccgaggtg
     4741 agtcgcatgg ccctcctgat tctaccctcc cgcg
//
LOCUS       DBNGZW010000339         3242 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1873375, whole genome shotgun sequence.
ACCESSION   DBNGZW010000339 DBNGZW010000000
VERSION     DBNGZW010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3242)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3242
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1873375"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="AC16JC_08045"
     CDS             complement(<1..428)
                     /locus_tag="AC16JC_08045"
                     /inference="COORDINATES: protein motif:HMM:NF022481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein O-mannosyl-transferase family"
                     /protein_id="HIR5164683.1"
                     /translation="MTHATDSTPQKPPYLWAAFVFLGTLALYVATLAPTTQFWDTSEY
                     IAAARVLGIPHPPGNPLFTMMAHVWGMLPLAAGYAQRINLFAATTSAVAAACWFLVGE
                     RWMRPIVPVPLVRRLAALGGALVSATAFTVWNQSVVNEKV"
     gene            complement(459..2600)
                     /locus_tag="AC16JC_08050"
     CDS             complement(459..2600)
                     /locus_tag="AC16JC_08050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 117 protein"
                     /protein_id="HIR5164684.1"
                     /translation="MTSGGPLEGPPYTAAALVAGGVLALYVATLAPTMQFWDAPEYMA
                     AAHALGIPHPPGNPLFTLLAHVWGLLPLAGDYGARINLFAAVTSAVAAGFWFLIAERW
                     LRPIVAGPAPRRLIAAAGAIIGATAFTVWNQSVVNEKVYTISLLSITLMLWLAIRWAD
                     HPAGERPDRLLVLIAYLLVLTSANHQMGLLAAPAILLLVVRTDARTLIRPRLVGVALL
                     AAAAAATVYLFLPIRAHLDPYLNEGDPSTWAALRDVLLRAQYGKPPVLQRQADLLGQL
                     AMWAQYFSWQWGRDLPDTAARALALGFGALGLLGAWRHWKAEPRQAAVMTTLVVTVTL
                     ALIFYLNFKYGYSEFPGRAVPREVRDRDYFFIVSFSAWGVWVAMGGAALMEWVGSRLP
                     RATPAPGWAWATPVLLIALVPVCGNRLSAPRRGETLARDYAVAVLQSLDPYAIVVTVG
                     DNDTFPLWYAQEVEGVRRDVTVLVTSLGNTNWYLRQLNQRPVAMFDSSTAPIWYRDGA
                     WPKPQTPWLGTLYRARTDTLPEYTALAHPVTGSLGPIAVSLDPATLPTPGFLSRVDLA
                     LLEIVKEQLGRRPIYFSSTTGNYGEQLGLGPFLVTEGMVRRLQPAPVTASTSVRPSAL
                     QGRYVDVPRTTRLGFELYHGAAAARARPRGWVDRASQNSLLPYIITYDTIAEALQNTD
                     PARAAQALALARAAMANTTYQFDLTPPAPAR"
     gene            complement(2718..>3242)
                     /gene="purH"
                     /locus_tag="AC16JC_08055"
     CDS             complement(2718..>3242)
                     /gene="purH"
                     /locus_tag="AC16JC_08055"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682768.1"
                     /note="involved in de novo purine biosynthesis; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR5164685.1"
                     /translation="RKKKNLRVVELPRPDDRATLDFKRVRGGLLAQDRFRPYDTSQES
                     LWKVVTERVPTESEWNDLRFAWAAVGSVKSNAILLARNEAAIGIGAGQMSRVDSSFLA
                     VHKARLAAHDTTGAVLASDAFFPFRDGVDEAAAAGVAAIIQPGGSVRDAEVIAAANER
                     GLAMVVTGVRQFRH"
BASE COUNT          525 a         1095 c         1193 g          429 t
ORIGIN      
        1 tacaccttct cgttcacgac cgactggttc cacacggtga acgccgtggc gctgacgagg
       61 gcgccgccca gcgccgcgag ccggcgcacc agcggcacgg gcacgatcgg gcgcatccag
      121 cgctcgccca ccaggaacca gcacgcggcc gcgacggcgc tggtcgtggc cgcgaacaga
      181 ttgatgcgct gcgcgtaccc ggcggcgagc ggcagcatcc cccacacgtg cgccatcatc
      241 gtgaacagcg ggttgccggg cgggtgggga atgccgagga cccgggccgc cgcgatgtac
      301 tcggaggtgt cccagaactg cgtggtcggc gcgagcgtcg cgacgtagag ggccagggtg
      361 cccaggaaga cgaaggcggc ccagagatag ggcggcttct gcggagtgct gtcggtcgcg
      421 tgggtcatgc ggttcgggtc agagaagaag agaaggtatc accgcgcggg cgcgggtggc
      481 gtcagatcga actggtaggt cgtgttggcc atggccgccc gcgccagggc gagcgcctgc
      541 gccgcgcggg ccggatcggt attctgcagc gcttcggcga tggtgtcgta ggtgatgatg
      601 tagggcagca ggctgttctg cgaggcgcgg tccacccagc cgcgggggcg cgcccgggcc
      661 gcggccgcgc cgtggtacag ctcgaacccc aggcgggtgg tccggggaac gtccacgtaa
      721 cgcccctgga gcgcgctggg tcgcacgctg gtggaggcgg tcacgggagc gggctgcagc
      781 cgccgcacca tcccctcggt cacgagaaaa ggtccgagcc cgagttgctc gccgtaattg
      841 ccggtggtgc tggagaaata gatcggccgc cgcccgagct gctccttcac gatctcgagc
      901 agggcgagat cgacgcgcga gaggaacccg ggcgtcggca gcgtggcagg gtcgagcgac
      961 accgctatcg gtcccagtga gccggtcacg ggatgggcca gggcggtgta ctccgggagc
     1021 gtgtcggtcc gcgcgcgata cagcgtgccg agccacgggg tctgcggttt gggccaggcg
     1081 ccgtcgcggt accagatcgg ggcggtgctc gagtcgaaca tcgcgaccgg ccgctggttc
     1141 agctgccgca ggtaccagtt ggtgttgccg agcgaggtga cgagcaccgt gacgtcgcgg
     1201 cgcacgcctt ccacttcctg tgcgtaccac agcggaaaag tgtcattgtc gccgacggtg
     1261 acgacgatcg cgtaggggtc gagcgattgc agcacggcga cggcatagtc gcgggccagc
     1321 gtctcgccgc ggcgcggcgc cgagagacga ttgccgcaga cgggcacgag ggcgatgagc
     1381 agcaccggcg tcgcccaggc ccagcccggc gccggcgtgg cgcgcggcag gcgtgatccc
     1441 acccactcca tcagggcggc gccgcccatg gcgacccaga cgccccaggc cgagaaggag
     1501 acgatgaaga agtaatcgcg gtcgcgcacc tcgcgcggca cggcccggcc gggaaactcg
     1561 gagtatccgt acttgaagtt gaggtaaaag atcagcgcga gggtgaccgt gacgacgagg
     1621 gtcgtcatca cggcggcctg gcgcggctcc gccttccagt ggcgccaggc gccgagcaag
     1681 cccagggcgc cgaagcccag ggccagcgcc cgggcggcgg tgtcgggcag gtcgcgtccc
     1741 cactgccacg agaaatactg cgcccacatc gccagctggc cgaggagatc ggcctgccgc
     1801 tgcagcaccg gcggcttgcc gtactgcgcc cgcagcagca cgtcgcgcag cgccgcccag
     1861 gtgctcgggt cgccctcgtt caggtatgga tcgaggtggg cgcggatcgg gaggaacaga
     1921 tagacggtcg cggcggcggc ggccgccagc agcgcgacgc cgacgagccg cggccggatc
     1981 agggtccgcg catcggtacg gacgacgagc agcaggatgg cgggcgctgc caggagtccc
     2041 atctggtgat tggcgctcgt caggaccagc agataggcga tgaggaccaa cagccggtcg
     2101 ggacgctcac ccgcgggatg atcggcccag cggatcgcca accagagcat cagggtgatc
     2161 gacagcaggc tgatcgtata caccttctcg ttcaccaccg actgattcca gaccgtgaac
     2221 gcggtggccc cgatgatcgc gcctgccgcg gcgatcaggc gccgcggcgc cggccccgcg
     2281 acgatcgggc gcagccagcg ctcggcgatg agaaaccaga agccggcggc cacggcgctc
     2341 gtgaccgcgg cgaagagatt gatgcgcgcg ccgtagtcgc ccgccagcgg gagcaggccc
     2401 cacacgtggg ccagcagcgt gaacagggga ttgccggggg ggtggggaat gccgagcgcg
     2461 tgcgccgccg ccatgtactc gggcgcgtcc cagaattgca tcgtcggagc cagggtcgcg
     2521 acgtacagcg cgaggacccc gcccgcgacg agcgccgccg cggtgtacgg aggaccttcc
     2581 agcggcccgc ctgatgtcat cctgagcgaa gcgaaggatc ccgagttcgc catatcaacg
     2641 acctttcaga ttcttcgccg cccgcggcgg ctcagaatga cgggggcgtg cgtccttccc
     2701 gcaccagcag taccccgtta gtgccggaat tgccggaccc cggtcaccac catggccagg
     2761 ccgcgctcgt tcgccgccgc gatgacttcc gcgtcgcgca ccgagccgcc cggctggatg
     2821 atggccgcca ccccggcggc cgccgcctcg tccaccccgt cgcggaacgg gaagaacgcg
     2881 tcgctcgcga gcaccgcgcc ggtcgtatcg tgcgcggcca gccgggcctt gtggaccgcg
     2941 aggaacgacg aatccacacg ggacatctgg ccggcgccga tcccgatcgc cgcctcgttc
     3001 cgcgccagga ggatcgcgtt cgacttcacg ctccccaccg ccgcccaggc gaagcgcagg
     3061 tcgttccact cggactcggt cggcacccgt tcggtcacga ccttccagag cgactcctgg
     3121 gaggtgtcgt agggccggaa ccggtcctgg gccagcagcc cgccgcgcac ccgcttgaag
     3181 tcgagggtgg cccggtcgtc gggacggggc agctcgacta cgcgcaggtt cttcttcttg
     3241 cg
//
LOCUS       DBNGZW010000340         7057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_431440, whole genome shotgun sequence.
ACCESSION   DBNGZW010000340 DBNGZW010000000
VERSION     DBNGZW010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_431440"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="AC16JC_08060"
     CDS             <1..188
                     /locus_tag="AC16JC_08060"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015042547.1"
                     /note="catalyzes the transfer of phosphate between the C1
                     and C5 carbons of pentose; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR5164686.1"
                     /translation="PTTPSTDHSREAVPILVLGDRIRPVSLGERATFADLGATVGEYF
                     GVAPLAAGRSFLSEVRA"
     gene            185..571
                     /locus_tag="AC16JC_08065"
     CDS             185..571
                     /locus_tag="AC16JC_08065"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011848708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR5164687.1"
                     /translation="MSDELVRRAQAVMENAYAPYSNFHVGAAIEADDGTIHVGCNVEN
                     ASYGLTICAERMAVGAAVAAGKRRLVRIAVASGIEPPATPCGACRQLLAEFGLDLEII
                     TAGPSSRRRWRLRELLPEAFTREALA"
     gene            568..1920
                     /locus_tag="AC16JC_08070"
     CDS             568..1920
                     /locus_tag="AC16JC_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164688.1"
                     /translation="MRGRHLRRSAVVLSIGALAALACFENPTSPAQCPDFCPTGGLAI
                     VESVLSTAIERDSSFRGYVQAHQSAMLIASSLPGVDSRPIWVSTHIQTRFLIDTGSDT
                     TTGPIVVDSMKLTLTVLRRDTTAVHNLRLAFYKLPVGIDSATTFADLATAFSGTPLRT
                     VNVDSLAALPGRRDTITGDTVLAVDSVHLHSVLLRIKFDSTRTGYSEADSGKLALGVI
                     ATGDSPVSIGLGAADGGFPSTAWWFIHVDSLGKRIRPDSLAHPVPGSDSLGAPPFFDS
                     FVFDAPPVIFDSNLTVGSAPSIRSLLRVNLPRGLRDSTQIIRATLQLVPAGAAVTTTD
                     SVFLRVKRLQADLGAKSPVVVTDTFVGSSAPFRGIPSDTVRIEVTSLFRVWQADTTAV
                     PAFYLSLLTLSRLQKDSIHLNGSEASTLAALRFYSSRSTAFSPSIRLTYVPRVKFGAP
                     "
     gene            1917..3026
                     /locus_tag="AC16JC_08075"
     CDS             1917..3026
                     /locus_tag="AC16JC_08075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164689.1"
                     /translation="MTITHRTACALAAVLLGATASSLQGQDSPFGINGLGTPGRFESA
                     RARATGGAFAAFDPLSALTDASIGGLGRLTATATGTTSYLSEDFGSVSASRRVSRFPL
                     FQVGGPAWSNVVLAAGISTYLDHTYHVRIEDTVSLGGSQQAVTDNLSGDGGVTDIRFV
                     AARRFGRLMVGAGFHLLSGSTRFQSVRNFTDTSAYGDVAQTVEVAYRGSGASASAMLN
                     VGRQLELAGFWRTDSRLRSEENGKTVAHSDLPTTYGGAIRWRPAPEAAFGAAVTYRGW
                     ASAADSNAYNTTNWSAGAEFGRRLPLRLGVRGGMLAFGPGGRAPRETAVAAGTGRVFA
                     GGRGIIDVTIEHLRRTGAGLTETAWTVLFGVAVRP"
     gene            2990..4240
                     /locus_tag="AC16JC_08080"
     CDS             2990..4240
                     /locus_tag="AC16JC_08080"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035596 - methylthiotransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HIR5164690.1"
                     /translation="MDRPVRRRRPAVNVYFHTFGCKANQYDTERVRQAFADAGVSVVD
                     APEAADLAVVNSCTVTHESEAKLRRYVRRQARAGRDAIVMGCAAALDDGALAALPGVR
                     AVVGGADPLPVLRAAGVSVDRVDPVLRRFSAGERGWLKIQDGCDEHCTFCATTVARGA
                     NRSRPVAEIVAEAQALAEHHAELVLTGVHIGTYGRDEGRGTRDEWSLGALLEALVRAV
                     PGVRFRLSSIEATEVDDRLARLLIEAPRHLAPHLHAPLQSGADRLLKRMGRHWYTAVS
                     YRARLEWLAVRLPVFGLGADVIAGFPGETAADHEATLALIDALPFTYLHVFPFSVRPD
                     APAARMAARVPEAIVRDRARELRALGEAKADAHRALRRGARADGVVCGRAGGQVDVLT
                     EDYLSVYLSSRAWDGRPRLDVTVD"
     gene            4243..5556
                     /gene="miaB"
                     /locus_tag="AC16JC_08085"
     CDS             4243..5556
                     /gene="miaB"
                     /locus_tag="AC16JC_08085"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HIR5164691.1"
                     /translation="MPRIYIETYGCQMNVADSEIMLGALGREGYVRTDDPAAADVLLV
                     NTCAVRDHAEQRVLGRMGELKRYKRPGGVLGVVGCMAQRLGPKLLERVPQVDLVIGPD
                     GYRGLPELIARARGGERAADTAFRSWEHYEDVPAVRGDTVSAFVTVQRGCDYRCTFCV
                     VPMTRGPERSRRLEDVVGEIGSLAARGTSEVTLLGQTVNSYHDGTHDFADLLRAVGAV
                     PGIRRVRFTSPYPTDFTDRVLSAIAETEAVCEHVHLPVQSGSSRVLKRMLRRYTRERY
                     LEVVDALRAAVPGIALTTDIIVGFPGETEADFAETLSLVEQVAFDDAYTFKYSVREDT
                     PAVRLKDHVPDAVAGERLERLVALVRAVARRRNMALVGTAHEVLVEGRARRGGMLQTR
                     TRTNKVALVDGPDHWIGTYRRVRFTGTTGSTFTAWPAEQEAALVG"
     gene            5568..5834
                     /locus_tag="AC16JC_08090"
     CDS             5568..5834
                     /locus_tag="AC16JC_08090"
                     /inference="COORDINATES: protein motif:HMM:NF022179.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HIR5164692.1"
                     /translation="MEATQKEYERLRAQYPEFCGCDTCRGDVQVFALNRLSPHYVSTR
                     AGEILTGITMGTDQETAKLTVILIEGFRRVALAPRCGAKPVTLA"
     gene            5849..>7057
                     /locus_tag="AC16JC_08095"
     CDS             5849..>7057
                     /locus_tag="AC16JC_08095"
                     /inference="COORDINATES: protein motif:HMM:NF015714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR5164693.1"
                     /translation="MRPPLLWIAIGFGAGLWAGLTSFGDGGRGTWEVGLPVLLGALLV
                     ARRTPVAAAMGVAAIAGLLWGMAAVARQDATCAGLWGRGTRDEGRGARAAIVRLLDRV
                     ADAGGVVDAEVRGGPCRGTLTLRWPSGKAAPGGTTWLVAGPYVGDAARGLLRVRSSRL
                     LDGIRRGRGGLRDRIAERSAALFGARAPLVDALVIGRTADVDSELRERYARSGLAHIL
                     SISGLHVGFFAAWLTLLLRLAGVPARPRLVCAGLAVFAYCWVIGFPAPATRAAVMLAL
                     DGVARWRQRLVAPRGTVALAAFVVLLADPWAIRSVGAWLSVSAIAAVIWAARAVGGPP
                     LLKLVAPAVAATLVTAPITAYAFGTVAPIGVIANLVAIPLGSIAVPGVIFALGLSSLV
                     PALGALLAAGA"
BASE COUNT          903 a         2444 c         2591 g         1119 t
ORIGIN      
        1 atccgaccac gccgtccacc gatcattcgc gcgaagcggt accgattctg gtgctggggg
       61 atcgcatccg acccgtgagc ctgggcgagc gcgcgacctt cgccgacctg ggcgccacgg
      121 tgggggagta cttcggcgtc gcaccgctcg ccgcggggcg gtcgttcctg tcggaggtcc
      181 gggcatgagc gacgagctgg tgcgccgggc acaggcggtg atggagaacg cctacgcgcc
      241 gtacagcaac ttccacgtcg gtgcggcgat cgaggccgac gacgggacga tccacgtggg
      301 gtgcaacgtc gagaacgcat cgtacgggct cacgatctgc gccgagcgga tggccgtcgg
      361 ggccgcggtg gcggcgggga agcggcgcct ggtgcggatc gcggtcgcca gcgggatcga
      421 gccgccggcc acgccgtgcg gggcctgccg ccagctgctg gccgagttcg ggctcgacct
      481 ggagatcatc acggcgggtc cgtcgagtcg gcggcgctgg cggttgcgcg agctcctgcc
      541 cgaggcgttc acgcgggaag cgctcgcatg agggggcgtc acctgcggcg cagcgccgtg
      601 gtgctgtcga tcggagcgct ggcggccctt gcctgcttcg agaacccgac ctcgccggcg
      661 cagtgccccg acttttgtcc caccggcggg ctggcgatcg tcgaatcggt gctctcgacg
      721 gcgatcgagc gggactcgtc gttccggggg tatgtgcagg cgcaccaatc tgcgatgctc
      781 atcgcgtcga gccttccggg agtcgactcg cggccgatct gggtctcgac tcacatccag
      841 acgcgatttc tgatcgacac cggcagcgac acgaccacgg ggccgatcgt ggtggactcg
      901 atgaagctca cgctgaccgt attgcggcgt gacaccaccg ccgtgcacaa tctccgcctc
      961 gccttctaca agctccctgt cggtatcgac agtgcaacca ccttcgccga tctcgcgaca
     1021 gcgttctccg ggacgccgct gcggacggtc aacgtggaca gcctcgccgc cctgcccgga
     1081 cggcgcgaca cgatcaccgg cgacaccgtc ctggccgtgg actccgtcca cctccactcg
     1141 gtgctgctgc ggatcaagtt cgacagcact cggacgggct acagcgaagc cgacagcggg
     1201 aagctcgcgt tgggcgtgat cgctaccggc gacagccctg tgagcattgg gttgggcgct
     1261 gcagacggcg gtttcccgtc cactgcctgg tggttcattc acgtcgattc gttggggaaa
     1321 cggatccgcc cggattcgct cgcgcacccg gttccgggat ctgactccct tggggcgccg
     1381 ccgttcttcg attcgttcgt gttcgatgcg ccgccggtga tattcgactc caatctgaca
     1441 gtcggcagcg caccctcaat caggtcactg ctccgtgtca acttgccgcg cggtctgcgc
     1501 gattcgaccc agatcatccg ggcgaccctg caactggtgc cggccggggc cgcggtgacc
     1561 accaccgatt cggtcttcct ccgggtgaag cgcctccagg cggatctcgg ggcgaagtcc
     1621 cccgtggtgg tcaccgacac gttcgttggt agctccgcgc cgttccgggg cattccatcc
     1681 gataccgtcc gcatcgaggt gacttcgttg tttcgtgtct ggcaagccga cacgaccgcc
     1741 gtgcccgcgt tctacctgag cctgctcact ctcagcaggc tccagaaaga ctcgattcac
     1801 ctgaacgggt cggaggcgag cacgctcgcc gctctgcgct tctattcctc acgctccacg
     1861 gccttcagcc catccatccg cctcacgtac gtgccgcgcg tgaagttcgg agcgccatga
     1921 ccataacaca ccggacagcg tgcgcgctgg cggccgtgct cctcggcgcc acggcgtcgt
     1981 ccctccaggg gcaggattcg cccttcggca tcaacggcct ggggacgccg ggccgcttcg
     2041 agagcgcgcg ggcccgcgcc acgggcggcg cgttcgcggc gttcgatccc ctgtcggccc
     2101 tcaccgacgc gtcgatcggc ggactgggtc ggctcaccgc gaccgccacc gggacgacgt
     2161 cgtatctgtc cgaggatttc ggcagcgtga gcgcctcgcg tcgggtctcg cggttcccgc
     2221 tgttccaggt cggcggcccg gcctggagca acgtggtgct ggccgccggc atctcgacct
     2281 acctcgacca cacctatcac gtcaggatcg aggacaccgt cagcctcggc ggctcgcagc
     2341 aggccgtcac cgacaacctc tcgggagatg gaggcgtcac cgacatccgg ttcgtcgcgg
     2401 cccggcggtt cgggcgcctg atggtcgggg ccggatttca tctgctgtcc gggtcgaccc
     2461 ggttccagtc cgtccgcaac ttcaccgaca cgtcggccta cggcgacgtg gctcagaccg
     2521 ttgaggtggc ctaccgtggc tccggggcat cggccagcgc gatgctgaac gtgggccgcc
     2581 agctcgagct ggccggattc tggcgcaccg actcccgcct gcgcagcgag gagaacggga
     2641 agacggtcgc ccacagcgac ctcccgacca cgtacggagg cgcgatccgc tggcggccgg
     2701 ccccggaggc cgcgttcggg gcagcggtga cgtaccgcgg ctgggccagc gccgcggact
     2761 cgaacgccta caacaccacg aactggtcgg cgggcgcgga gttcggccgc cgcctgccgc
     2821 tgcgcctcgg cgtgcgtggg gggatgttgg cgttcgggcc gggcggccgc gcgccgcgtg
     2881 aaaccgccgt tgctgcgggc acggggcgcg tgttcgcggg cgggcgcggc atcatcgacg
     2941 tgacgatcga acacctgcgg cgcaccggcg ccgggctgac cgagacggcg tggaccgtcc
     3001 tgttcggcgt cgccgtccgg ccgtgaacgt ctacttccat acgttcggct gcaaggccaa
     3061 ccagtacgac accgagcgcg tccgccaggc gttcgccgat gccggcgtca gcgtggtgga
     3121 cgcgcctgaa gcggccgatc tcgcggtggt caattcctgt acggtgacgc acgagagcga
     3181 ggcgaagctg cgccgctacg tgcggcgaca ggcacgcgcc ggccgggacg ccatcgtgat
     3241 ggggtgcgcc gccgcactgg acgacggagc gctcgccgcg ctccccggcg tgcgggccgt
     3301 cgtcggcggc gccgatccgt tgccggtgct gcgcgcggcg ggggtgtcgg tcgaccgcgt
     3361 ggatcccgtg ctgcgccgct tcagcgcggg cgagcgtggc tggctcaaga tccaggacgg
     3421 ctgcgacgag cactgcacct tctgtgcgac gacggtcgcc cgcggcgcca accgctcgcg
     3481 tcccgtcgcg gagatcgtcg ccgaagcgca ggcgctggcg gagcaccacg cggagctcgt
     3541 actcaccggc gtgcacatcg ggacctacgg aagggacgag ggacgaggga cgagggacga
     3601 gtggtcgctg ggtgctctcc ttgaagcgtt ggtgcgtgcg gtgcccggcg tacggttccg
     3661 cctctcctcg atcgaggcga ccgaggtgga cgatcgcctg gcccggctgc tgatcgaggc
     3721 gccgcggcac ctggcaccgc acctgcacgc gccgctgcaa tccggcgccg atcgcctgct
     3781 caagcgcatg gggcggcact ggtacacggc ggtctcctac cgggcgcgcc tcgagtggct
     3841 ggcggtgcgg ttgccggtgt tcggcctggg ggccgacgtg atcgccgggt tccccgggga
     3901 gaccgccgcc gatcacgagg ccacgctcgc actcatcgac gccctgccgt tcacctacct
     3961 ccacgtgttt ccgttcagcg tgcggccgga cgcaccggcg gcccggatgg cggcgcgggt
     4021 gccggaggcg atcgtgcggg atcgagcgcg ggagctgcgc gcgttgggtg aggccaaggc
     4081 cgacgcccac cgggcgttgc gccgcggcgc gcgtgccgac ggcgtcgtgt gcggccgcgc
     4141 tggcggtcaa gtggatgtgc tgacggagga ctacctctcg gtctatcttt cgagccgggc
     4201 ctgggacgga cgtccccggc tggacgtgac cgtggactga ccgtgccccg catctacatc
     4261 gaaacgtacg gctgccagat gaacgtcgcg gactccgaga tcatgctcgg agcgctgggg
     4321 cgggaagggt acgtccggac tgatgatccg gcggcggcgg acgtgctgct ggtcaatacc
     4381 tgtgcggtgc gcgaccacgc cgagcagcgc gtgctgggcc gcatgggcga gctgaagcgc
     4441 tataagcgcc cgggcggggt gctcggggtc gtgggctgca tggcccagcg tctcgggccg
     4501 aaactgctgg agcgtgtccc ccaggtggac ctggtcatcg gccccgacgg ctaccgtggc
     4561 ctgcccgagc tgatcgcgcg ggcccgcggt ggggagcgcg ccgccgacac cgccttccgg
     4621 agctgggagc actacgagga cgtcccggcg gtacgggggg acacggtctc ggcgttcgtc
     4681 acggtgcaac gcggctgcga ttatcggtgc accttctgcg tggttccgat gacgcgcggg
     4741 ccggagcgga gtcgcaggtt ggaggacgtg gtgggcgaga tcgggagtct cgccgcccgg
     4801 ggcacctccg aggtcacgct ccttggccag acggtcaaca gctaccacga cggcacgcac
     4861 gacttcgccg acctgctgcg cgcggtgggc gcggttccgg gcatccggcg ggtgcgcttc
     4921 acctcaccct atcccacgga cttcacggac cgggtcctct cggccatcgc cgagaccgag
     4981 gccgtgtgcg agcacgtaca tctgccggtg cagagcggca gctcgcgggt gctcaagcgc
     5041 atgctgcgcc gctacacgcg cgagcgctac ctcgaggtgg tggacgcctt gcgtgcggcg
     5101 gtgccgggca tcgcgctcac gaccgacatc atcgtcggct tccccggcga gaccgaagcc
     5161 gacttcgccg agacgctgtc gctggtggag caggtggcgt tcgacgacgc gtacaccttc
     5221 aagtactccg tgcgcgagga cacccccgcg gtccgcctca aggatcacgt gcccgacgcc
     5281 gtggcgggcg agcggctgga gcggctcgtc gcgctggtcc gtgccgtggc ccgccgccgc
     5341 aacatggcgc tggtgggaac cgcacatgaa gtgctggtcg aagggcgcgc caggcgcggc
     5401 ggcatgctgc agacccgcac gcgcaccaac aaggtggcgc tggtcgatgg ccccgatcac
     5461 tggatcggga cctaccggcg ggtccggttt accggcacga ccgggtccac cttcaccgcg
     5521 tggccggcgg aacaggaggc tgcgctcgtg gggtaacgaa cgtcactatg gaagcgacgc
     5581 agaaggagta cgagcgcctg cgggcgcagt accccgagtt ctgcggctgc gacacctgcc
     5641 gcggcgacgt ccaggtgttc gcgctgaacc ggctgtcgcc gcactacgtg tccacgcgcg
     5701 ccggcgagat tctcacgggc atcacgatgg ggaccgatca ggagaccgcg aagctcaccg
     5761 tcatcctgat cgagggcttt cgtcgcgtgg ccctggcgcc gcgttgcggg gcgaaaccgg
     5821 tcacgctcgc gtaagggcgt aggcggcggt gcgcccgccg ctcctgtgga tcgcgatcgg
     5881 cttcggggcc ggcctgtggg ccggcctcac ctctttcggg gacgggggac gggggacgtg
     5941 ggaggtgggg ctcccggtgc tgttgggggc gctgctcgtg gcgcggcgga cgcccgtggc
     6001 ggcggcgatg ggcgtcgcgg cgattgccgg gctcctctgg ggaatggcgg cggtggcgcg
     6061 gcaggatgcg acatgcgcgg gcctgtgggg acgggggacg agggacgagg gacgaggcgc
     6121 gcgcgccgca atcgtgcggc tgctcgatcg cgtggccgac gcgggtggcg tggtggacgc
     6181 ggaggtgcgc ggcgggccgt gccgcggcac gctcacgctg cgctggccca gtgggaaggc
     6241 cgcgccgggc gggaccacgt ggctcgtcgc gggaccttat gtcggggacg cggcccgcgg
     6301 gctgctgcgc gtgcggtcca gccgtctgct cgatggcatt cgacgcgggc gcggcggcct
     6361 gcgcgaccgg atcgccgagc gtagcgcggc gctgttcggc gcccgcgcgc cgttggtgga
     6421 cgcgctcgtc atcggacgga ccgccgacgt cgactccgag ctgcgcgagc gctacgcccg
     6481 ctccggcctc gcgcatatcc tctcgatttc cggattgcac gtcggcttct tcgccgcctg
     6541 gctgacgctg ctgctccgcc tggccggggt tcccgcgcgc ccgcgcctcg tctgcgcggg
     6601 gctggcggtg ttcgcctact gctgggtgat cggcttcccg gcgccggcca cgcgggcggc
     6661 ggtgatgctc gccctggacg gggtggcccg gtggcgccag cgcctcgtgg cgccgcgcgg
     6721 cacggtcgcg ctggccgcgt tcgtcgtgct cctcgccgac ccctgggcca tccgctcggt
     6781 cggagcgtgg ctctcggtgt cggcgatcgc ggcggtcatc tgggccgccc gggccgtcgg
     6841 cggtccgccg ctcctcaagc tggtcgctcc cgccgtcgcg gccacgctcg tcacggcccc
     6901 gatcaccgct tacgccttcg gcacggtggc gccgatcggc gtgatcgcca atctggtggc
     6961 gatcccgctc gggtccatcg ccgtccccgg cgtcatcttc gcccttggcc tgtcgagtct
     7021 cgtgcccgcc ctcggcgccc tgctcgccgc cggcgcg
//
LOCUS       DBNGZW010000341         1435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_917608, whole genome shotgun sequence.
ACCESSION   DBNGZW010000341 DBNGZW010000000
VERSION     DBNGZW010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_917608"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..840)
                     /locus_tag="AC16JC_08100"
     CDS             complement(<1..840)
                     /locus_tag="AC16JC_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HIR5164694.1"
                     /translation="MDDGSGHLEGLNSGQRAAAEYGTASLPGELPGPLLIIAGAGTGK
                     TTTLAHRVAHLIVRGADARRILLLTFTRRAAESMARRAAQIVAQVVRNGAKPPRVEWS
                     GTFHAVANRLLRHHADSLGLDPAFTVLDRSDSADLLNLVRNELGLSKQATRFPRKDTC
                     LAIYSHAVNSGLPVEQTLERAFPWCGTWADELKRLFRTYVDAKQRQHLLDYDDLLLYW
                     FHLMQAGVPAEAVRRRFDHVLVDEYQDTNALQAGILRGLKPDCRGLAVVGDDAQSIYS
                     FRAA"
     gene            complement(894..>1435)
                     /locus_tag="AC16JC_08105"
     CDS             complement(894..>1435)
                     /locus_tag="AC16JC_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HIR5164695.1"
                     /translation="LWYARPNGTYWTVQQGRAAGTLTDRAYFPKVLRGQTVLGDLVVS
                     HSTSRNTAIVAVPVRGRGNAVTGVLGASVHLDSLGGLLRRELGGLEERLIFFAIDSTP
                     IGALNSDPNLIFTEPMKLGDEDMRAAFTEILKRNEGAVIYNFRGHLRTVLYRRSPVTG
                     WWYAFGAVGTAAEATGGGR"
BASE COUNT          222 a          487 c          506 g          220 t
ORIGIN      
        1 agccgctcgg aacgagtaga tcgactgcgc atcgtcgccc acaaccgcga gaccgcggca
       61 gtcgggcttg aggccacgca ggatgccagc ctggagcgcg ttggtgtcct ggtattcgtc
      121 caccagcacg tggtcgaagc ggcgccgcac ggcttcggcc ggcacgcccg cctgcatcag
      181 gtggaaccag tagaggagca ggtcgtcgta atcgagcaga tgctggcgct gtttcgcgtc
      241 cacgtaggtc cgaaagagcc gcttgagctc gtcggcccag gtgccgcacc aggggaacgc
      301 gcgctcgagc gtctgctcga ccggaagtcc ggaattcacc gcgtgcgagt agatcgcgag
      361 gcaggtgtcc ttccggggga agcgtgtggc ctgcttcgac agtcccagct cgttgcggac
      421 gagattgagg aggtctgccg agtcggaccg gtcgagcacg gtgaaggcgg ggtcgaggcc
      481 cagggaatcg gcatgatgcc gcagcaggcg attcgcgacg gcgtggaacg tccccgacca
      541 ctcgacccgc ggcggcttgg ctccgttccg gacgacctgg gccacgatct gcgccgcgcg
      601 ccgcgccatc gattcggctg cgcgccgcgt gaaggtgagg agcagaatac gccgcgcgtc
      661 cgcgccgcgc acgatcaggt gcgcgacccg gtgcgccagg gtggtcgtct tgccggttcc
      721 cgcgccggcg atgatgagca gcgggccggg gagctccccc ggcagcgatg cggtgccgta
      781 ctccgcggcc gcccgctggc ccgaattgag cccctcgagg tgcccgctgc cgtcgtccat
      841 ggtgccccga atgtaacccg aagaggcggg gcaccgccaa cttcaggccg gacttaccgg
      901 ccgccgcccg tcgcctccgc tgccgtgccc acggctccga acgcgtacca ccacccggtt
      961 accggggatc tccgatagag cacggtgcgg agatgtccgc ggaagttgta gatcacggct
     1021 ccctcgttcc gcttcaggat ctcggtgaac gccgcccgca tgtcctcatc gcccagcttc
     1081 atgggctcgg tgaagatcag gttcgggtcc gagttgagcg ccccaatcgg cgtggagtcg
     1141 atggcgaaga agatcagccg ctcctccagc cctcccaact ctcgccgcag cagcccgccg
     1201 aggctgtcca gatgcacgga ggcgccgagc acgcccgtga cggcgttgcc gcggccgcgc
     1261 accggcacgg cgacgatcgc ggtgttgcgg ctcgtggaat ggctgaccac caggtcgccg
     1321 agcacggtct gtccgcgcag caccttgggg aagtacgcgc ggtcggtgag ggtgccggcg
     1381 gcccgcccct gttgcacggt ccagtaggtg ccgttgggcc tggcgtacca gagca
//
LOCUS       DBNGZW010000342         5976 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_783869, whole genome shotgun sequence.
ACCESSION   DBNGZW010000342 DBNGZW010000000
VERSION     DBNGZW010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5976)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5976)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5976
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_783869"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="AC16JC_08110"
     CDS             <1..757
                     /locus_tag="AC16JC_08110"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR5164696.1"
                     /translation="KAPLVAVVSQSLAAANFQDRLGVGRRIQVGVAPTRWYTVVGVVA
                     DQLPAGFGAGFQPRAAVYLSVLQHPIPAVDLLVRARGDSSVYGAVERQLRASLGKSAA
                     ATTRVGESRLLAAEAAPIRWFGRLFGLEGWVMLGAATIGTFVVLRLWVRSLLHDLGIR
                     RAVGARRRHLYGFVFARALGISVAGVALGCWCGAMLWGALSSVVAGLPGWDAGIAERY
                     GALLALAALLGALLPAWEAAHAAPARLVGHEEA"
     gene            828..1073
                     /locus_tag="AC16JC_08115"
     CDS             828..1073
                     /locus_tag="AC16JC_08115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164697.1"
                     /translation="MEFESLLVLLAVRGAPLDGSALDELANARATWVRLEREGEAVCL
                     AARVRHGDLPEEFRTRVRGWAAARGWAVTVAPCAPSL"
     gene            1084..1857
                     /gene="lepB"
                     /locus_tag="AC16JC_08120"
     CDS             1084..1857
                     /gene="lepB"
                     /locus_tag="AC16JC_08120"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011341348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR5164698.1"
                     /translation="MHDLGAPVEGPGANQALGGLVAPERRRSRFARWFDSWMRSLGMA
                     LVLFLIIKTFFFEAFQIASGSMTHTLLTGDFLFVNKALYGAQIPGTRARLPGFRSPRR
                     GDVIVFAYPRDPRQDYVKRVIGTPGDTVQMRSGRVTVNGRLLREPYVQRIDPGNDDVR
                     SEFEWQRSFYLAPTEQDRLRYRPSRDTWGPLLVPEGKYFVLGDNRDDSSDSRYWGFVD
                     ASAVKGRTLLVYFSYDRETHDALPWLTDIRWSRLGSLIH"
     gene            1954..2811
                     /locus_tag="AC16JC_08125"
     CDS             1954..2811
                     /locus_tag="AC16JC_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5164699.1"
                     /translation="MRLNTAKRVSFDEGSLDQYLREISQYPLITREDEVELAQRIRRS
                     DPEALDKLVRSNLRFVVSVAKKYQNQGVSLSDLINEGNLGLIRAAHKFDETKGIKFIS
                     YAVWWIRQAILQALAEQSRIVRVPLNRAGTLHRIGKRSSALLQELGREPTVEEIAEGM
                     DISQEEVAKTLSISQAHLSLDAPLTPGEDNKLLDYLPDTQNPGPDDEIFEHALTESIE
                     GVLSTLKEREAKILRLYFGLEGQEPMTLEEIGSLLGITRERVRQIKEKALARLRHVSR
                     ARALESFLS"
     gene            2820..3314
                     /locus_tag="AC16JC_08130"
     CDS             2820..3314
                     /locus_tag="AC16JC_08130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010943822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HIR5164700.1"
                     /translation="MSSFDISTGADLQEVDNAVNQATKEVAQRYDFKGSKCAITFDRA
                     KATLTLVADDDFKMKALFDVLQGKLIKRGVPVKNLEPGEVTAAAGDTVRREIKLTQGI
                     SQEKSKAIVKAIKDRKFKKAQASIQGEEVRVQSPSKDELQEVMAFLRTQDFGIELRFG
                     NYRG"
     gene            3311..4648
                     /gene="rimO"
                     /locus_tag="AC16JC_08135"
     CDS             3311..4648
                     /gene="rimO"
                     /locus_tag="AC16JC_08135"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR5164701.1"
                     /translation="MKLGVISLGCDKATVDSERLVGELVAHGAVVTPDLPAADVVLVN
                     TCGFIDAAKQESINTILEAAQLKRSGAVKAVVAVGCLVQRHRDDLVREMPEVDLFLGF
                     SDLHHLVPELARRGLIADPVERHPGVRRFLGDQPHVRYLKISEGCDHTCAFCAIPLMR
                     GKHRSEPLERLVREAQGLEAQGAKEINLVAQDLGHWGRDLGLGGPKLPDLIGTLLAET
                     SVPWYRLLYVYSAGISERLVDLMAREPRIVPYIDMPIQHASDRMLERMRRPERQASLR
                     EKLAWLRGAVPDLAIRTTCLVGFPGETDGDFRELLAFLEEAQFDRLGAFAYSPQEGTR
                     AVAYPDDVPDGEKQDRLEELLEVQRAISAERLARFVGREARVLIDRVADPLDGGVAGG
                     GGGATHVGRVMWQADDVDGVTYLGNGGWAETGSFVRARLTGSLDYDFQAVAIA"
     gene            4645..5937
                     /locus_tag="AC16JC_08140"
     CDS             4645..5937
                     /locus_tag="AC16JC_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030435 - sporulation resulting in
                     formation of a cellular spore [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR5164702.1"
                     /translation="MSRAGGGTARRGASRGWHGAPFSWAATASLIALAACPRPQPGVV
                     GPEPELRVGIVTGAAGVRLGGDGELFVTDDANGQPVGAIPAGTTWSVVPDSGGGLVLV
                     RANGTSTERHGGISVVNVTEGRFAMANGQRYRGRVNVVRDRTGLTLISRLPLESYLAG
                     VLSREMGPRAPEEREALLAQTVVARTFAMRNRGRWEADGFDATADTRDQVYLGVEGET
                     QQAWDAVRATAGLVLRYHGQLIDAYYHSTCGGRTAAVEEVFRSAQRRPYLRSVSDASG
                     GGHSYCERSPRFRWRAQWDAPMLRAILTRTLTTVMPVSGDGLQRITDVEVTRTTSSGR
                     VSELRIAFPHGDVRVAGPDVRTVLRPAPDQLLQSTAFRVFVTRDSSGVARLMAEGTGS
                     GHGVGFCQWGAIGRARAGQRYRQILTTYFPGTTVEKLY"
BASE COUNT          893 a         1936 c         2187 g          960 t
ORIGIN      
        1 gaaggcgccg ctcgtcgccg tggtgagcca gtcgctcgcc gcggcgaact tccaggatcg
       61 actgggcgtg ggccgacgca tccaggtcgg agtcgccccg acccgttggt ataccgtcgt
      121 gggcgtggtc gccgatcagc tgccggcggg gttcggcgcc ggtttccagc cgcgggcggc
      181 cgtctacctg agcgtgttgc agcatccgat accggccgtg gacctgctgg tgcgagcccg
      241 cggcgattcg agcgtgtatg gcgccgtcga gcggcagctg cgggcctcgc tggggaagag
      301 tgctgcggcg acgacgcggg tcggcgaatc acgcctgctg gccgccgagg ccgccccgat
      361 ccggtggttc gggcgcctgt tcggactgga agggtgggtg atgctcgggg cggcgacgat
      421 cgggacgttc gtcgtcctgc ggctgtgggt ccggtccttg ctccacgatc tcggcatccg
      481 gcgggccgtg ggagcccgac ggcgacatct gtacgggttc gtgttcgcgc gggcgctggg
      541 gatctccgtc gcgggtgtgg cattggggtg ctggtgcggt gcgatgctct ggggagcgct
      601 gtcgtcggtc gtggccgggt tgccgggctg ggatgcgggc atcgcggagc gatacggcgc
      661 gttgctcgca ttggcggcgc tgttgggcgc gctcctgccg gcatgggaag ccgcccatgc
      721 cgcgccggcg cggctcgtcg gccacgagga ggcgtgaggc gtgaaaaggt gccgttccgc
      781 atgcatcgga tgacgatcgt gggtgcccga cgcggtaccg tggcgtcgtg gaattcgaga
      841 gcctcctcgt cctgctcgcc gtgcgcggcg cgccgctcga tggttcagct ttggacgagc
      901 tcgccaacgc ccgggcgacc tgggtccggc tggaacggga aggcgaggct gtgtgcctcg
      961 ccgcccgtgt gcggcacggc gacctgccgg aggagttccg cacccgggtg cgggggtggg
     1021 ccgcggcacg ggggtgggcg gtgaccgttg cgccttgcgc tccatccttg taaagtgaat
     1081 cgcatgcatg acttgggagc cccggtggaa ggtccgggcg caaatcaggc gctcgggggg
     1141 ctcgtcgccc cggaacggcg tcgctcccgc ttcgcgcgct ggttcgacag ctggatgcgc
     1201 tccctcggca tggcgctggt gttgtttctc atcatcaaga cgttcttctt cgaggcgttc
     1261 cagatcgcca gcggcagcat gacgcacacc ctgctcaccg gcgatttcct gttcgtcaac
     1321 aaggcgctct acggcgcgca gattcccggg acccgggcgc ggctgccggg cttccgcagc
     1381 ccgcgccgcg gcgacgtcat cgtcttcgcg tatccacgcg acccccgcca ggattacgtg
     1441 aagcgggtga tcggcactcc cggcgacacg gtccagatgc gctcgggccg ggtgacggtg
     1501 aacggccggt tgctgcggga gccgtacgtg caacgcatcg acccgggcaa cgacgacgtg
     1561 cgttcggagt tcgaatggca gcggtccttc tacctcgcgc cgacggagca ggaccgcctt
     1621 cgctatcgtc cgagtcgtga cacctggggg ccgctcctcg tgcccgaggg gaagtatttc
     1681 gtcctgggcg acaaccgcga cgattcgtcg gattcccgct attgggggtt tgtcgacgcg
     1741 tcggcggtga aaggacggac actgctggtc tatttcagct atgatcggga gacgcacgac
     1801 gcgctcccct ggttgaccga catccggtgg agccggctcg ggtcgttgat tcactgaccg
     1861 ggcggtaagc cggggcggtc ccgcgtcctc ctctctggtg gagcgcgtgg ggctattccg
     1921 gtgcagccgc cgtcacggag ggatggccat tccatgcgct tgaacacggc gaagcgggtc
     1981 tcgttcgacg agggatcgct cgatcagtac ctgcgggaaa tcagccagta ccccctgatc
     2041 acccgggagg acgaggtcga gctggcccag cgcatccggc gcagcgaccc ggaagcgctc
     2101 gacaaactgg tacggtccaa cctgcggttc gttgtcagcg tcgccaagaa gtaccagaac
     2161 cagggcgtct cgctgtcgga cctgatcaac gaaggcaatc tcgggctgat ccgggcggcc
     2221 cacaaattcg acgagaccaa gggcatcaag ttcatcagct acgcggtgtg gtggatccgc
     2281 caggcgatcc tgcaggccct ggcggagcag tcgcgcatcg tccgcgttcc cctgaaccgc
     2341 gcgggcacgc tgcatcgcat cgggaagcgc tcctcggcgc tgctccagga gctgggtcgc
     2401 gagccgacgg tcgaggagat cgccgagggc atggacatct cgcaggagga ggtggccaag
     2461 accctgtcga tctcccaggc gcacctgtcg ctcgacgccc cgctcacgcc gggggaggac
     2521 aacaagctgc tcgactacct gcccgacacg cagaatccgg ggccggacga cgagatcttc
     2581 gagcacgccc tcaccgaatc gatcgagggg gtcctctcca cgctgaagga gcgggaggcc
     2641 aagatcctgc gcctctattt cggcctcgag gggcaggagc cgatgacgct cgaggaaatc
     2701 ggatcgctgc tcggcatcac gcgcgagcgc gtgcgtcaga tcaaggagaa ggcgctcgca
     2761 cggctgcggc atgtcagccg ggcccgcgcc ctggagagct tcctgtccta gtggcttccc
     2821 tgtcctcctt cgacatctcc accggcgccg atctccagga agtcgacaac gccgtcaatc
     2881 aggcgaccaa ggaggtcgcc cagcgctacg acttcaaggg ctcgaagtgc gcgatcacct
     2941 tcgaccgcgc caaggccacc ctgaccctcg tcgccgatga cgacttcaag atgaaggcgt
     3001 tgttcgacgt gctgcagggg aagctcatca agcgcggcgt gccggtgaag aatctcgagc
     3061 ccggggaggt gacggcggcg gccggggata ccgtacggcg cgagatcaag ctgacgcagg
     3121 gcatttccca ggagaaatcc aaggccatcg tgaaagccat caaggatcgg aagttcaaga
     3181 aagcccaggc ctcgatccag ggagaggaag tgcgcgtgca gtcgccctcc aaggacgagc
     3241 tgcaggaagt catggcgttc ctgcggacgc aggacttcgg catcgagctg cggttcggga
     3301 actaccgcgg atgaagctgg gcgtcatctc gctcggctgc gacaaggcca ccgtggattc
     3361 cgagcggctc gtgggcgagc tggtggccca cggcgccgtc gtgacccccg atctccccgc
     3421 cgccgacgtg gtgctggtca acacctgcgg cttcatcgac gcggcgaagc aggagtccat
     3481 caacaccatc ctcgaagcgg cgcagctcaa gcgcagcggt gcggtgaagg ccgtggtcgc
     3541 ggtgggctgc ctggtccagc gccatcgcga cgatctcgtc cgggaaatgc cggaggtgga
     3601 cctgttcctc gggttctccg acctccacca cctcgtcccc gagctcgcgc ggcgcggcct
     3661 catcgccgac ccggtcgaac gtcatcccgg cgtacgccgg ttcctcggtg atcagccgca
     3721 cgtccgctac ctcaagatct cggaaggctg cgaccatacc tgcgccttct gcgccattcc
     3781 cctgatgcgc ggcaagcacc gctccgagcc gctggagcgt ctggtgcgag aggcgcaggg
     3841 actggaagcg cagggggcga aggagatcaa cctggtggcg caggacctcg ggcactgggg
     3901 gcgggacctg ggactcggcg gtcccaagct ccccgatctg atcgggacgt tgctcgccga
     3961 gacgtcggtc ccctggtacc ggctgctgta cgtgtattcg gcgggtatca gcgagcgact
     4021 cgtggacctg atggcccggg agccccgcat cgtgccctac atcgacatgc ccatccagca
     4081 cgccagcgac cgcatgctcg agcgcatgcg gcgccccgag cgccaggcga gcctgcggga
     4141 gaagctggcg tggttgcgcg gggccgttcc cgacctggcg atccgcacca cgtgtctcgt
     4201 gggcttcccg ggggagacgg atggcgactt tcgcgagctg ctggcgtttc tcgaggaggc
     4261 ccagttcgac cggctcgggg cgttcgccta ttccccgcag gaggggacgc gcgccgtcgc
     4321 gtaccccgac gacgtccccg acggggaaaa gcaggatcgg ctcgaggagt tgctggaggt
     4381 gcagcgcgcg atctcggccg agcgcctggc ccgcttcgtc ggtcgcgagg cacgcgtgct
     4441 aatcgaccgc gtggccgacc cgctggacgg aggcgtcgcg ggcggcgggg gaggggccac
     4501 gcacgtaggg cgcgtcatgt ggcaggcgga cgacgtggat ggggtcacct acctgggaaa
     4561 cggtggctgg gcggagaccg ggagcttcgt gcgcgcgcgg ctgaccggca gtctggatta
     4621 cgattttcag gcggtggcga tcgcatgagc cgggcgggcg gagggaccgc tcgcaggggg
     4681 gcgtcccgcg gatggcacgg ggcgcctttt tcgtgggcgg cgacggcctc cctgatcgcg
     4741 ctggccgcgt gccccaggcc ccagcccgga gtcgtcgggc ccgagcccga gctgcgcgtc
     4801 ggcatcgtca cgggggctgc cggcgttcgg ctcggaggag acggcgagct gttcgtgacc
     4861 gacgacgcca acggacagcc ggtgggggcg atcccggccg gtacgacttg gtcggtcgtc
     4921 ccggactcgg gcggcgggct ggtgctggtg cgcgccaacg gcaccagcac cgagcgccac
     4981 gggggcatct ctgtggtgaa cgtgaccgaa ggccgcttcg ccatggcgaa cggacagcgc
     5041 taccgcgggc gggtgaacgt ggtccgtgac aggaccggcc tcacgctgat cagccggctg
     5101 ccgctcgaga gctatctcgc aggcgtgctg agccgggaga tggggccgcg ggcgcccgag
     5161 gagcgtgagg cgttgctggc gcagaccgtc gtggcccgca ccttcgcgat gcgcaaccgc
     5221 ggacgctggg aggccgacgg cttcgatgcg accgccgaca cgcgcgatca ggtgtacctg
     5281 ggggtagagg gcgagacgca acaggcgtgg gacgcggtgc gcgcgacggc cggactcgtc
     5341 ctgcgctacc acggtcagct gatcgatgcg tactaccact ccacctgcgg cggccgcacg
     5401 gccgcggtgg aggaggtgtt ccgctccgcg cagcgccggc cgtacctgcg gtcggtgtcg
     5461 gatgcgagcg gcggcggcca ctcctactgc gagcggtcgc cgcgcttccg atggcgggca
     5521 cagtgggatg ctcccatgct gcgcgctata ttgacgcgga cgctgacgac cgtgatgccg
     5581 gtgagcggcg acgggctgca gcgcatcacg gacgtcgagg tgacgcggac gacgtcctcg
     5641 ggccgcgtga gcgagctgcg tatcgcgttc cctcacgggg acgtccgcgt cgccggtccc
     5701 gacgtgcgga ccgtgcttcg gccagcgccc gatcagctgt tgcagagcac cgcgttccgg
     5761 gtgttcgtca cccgcgattc cagcggggtc gcgcggctca tggcggaagg caccggctcg
     5821 ggccacggag tgggcttctg ccagtgggga gccatcgggc gggcccgcgc gggacaacgc
     5881 taccggcaga tactcaccac ttacttcccg gggacgaccg ttgaaaagct ctactagaac
     5941 gatccccctg ctcgtgatat tgaccggcgg gctcgc
//
LOCUS       DBNGZW010000343         2176 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_704898, whole genome shotgun sequence.
ACCESSION   DBNGZW010000343 DBNGZW010000000
VERSION     DBNGZW010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2176)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2176)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2176
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_704898"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..1128
                     /gene="selD"
                     /locus_tag="AC16JC_08145"
     CDS             82..1128
                     /gene="selD"
                     /locus_tag="AC16JC_08145"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:124..126,aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HIR5164703.1"
                     /translation="MTTVRLTAFSHGAGUACKLGPSELTQVLRHVPLPTDPRILVDAA
                     TRDDAAVFQVAPDRALVATVDFFTPIVDDAYDFGRIAAANAFSDVYAMGAVPLFALNV
                     VGWPRERLPLELLGDVLRGGADVARAAGAFVVGGHSVDDPEPKYGMVVIGEVHPERMV
                     TIAGARPGDVLVLTKPIGTGILTTALKRDLLPASALVEAVEAMTTLNAGASRAMLATG
                     VHAATDVTGFGLLGHLRNMLDASGVAAEVEAARVPLLPHTREMLAKGAVPGGSSRNLE
                     SVAAVVRFPEGATKADRLLLADAQTSGGLLISLAAERVPRLQEALQREGQAPAAVIGR
                     VVAGPEQAGSIVVR"
     gene            1138..1374
                     /locus_tag="AC16JC_08150"
     CDS             1138..1374
                     /locus_tag="AC16JC_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164704.1"
                     /translation="MGVIPEYRSLTPDALDALERAVRERRRVALSRRGTEYVVVAERL
                     DVADRDETLQARLPMTGELLSFRLRDLETFAVLP"
     gene            1403..2152
                     /locus_tag="AC16JC_08155"
     CDS             1403..2152
                     /locus_tag="AC16JC_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5164705.1"
                     /translation="MFTRLLVGLDGSPHGDAALEQAVVLARRFRSVIVVAVVAEDDAA
                     GATVLLERGRFRVQAAGLEVEAVERQGSPDVNLAELAKEVDAALVGRRGLHTEREGPS
                     AIGHTAASLIRLAERTVIVCGGVASPMRSCAVAFDGRNRHALELAARFASVAGSTVHV
                     IHAYRDRDEGTLVAGQAEAELSLLGVQFQTHIAEGSAGEVIARLVREVHADALFAGAH
                     VARGLGERRPSVASHTEEILLHTDLPVAIQP"
BASE COUNT          285 a          758 c          794 g          339 t
ORIGIN      
        1 aaagggcgga acgagagaca tcagttccgc ccttttccgc cttcttccgc cttcttccgc
       61 cctctccata tttcgcctcc catgacaacc gtccgcctca cggccttttc gcacggcgcc
      121 ggctgagcct gcaagctcgg tccgtccgag ctgacgcagg tcttgcgtca cgtgccgttg
      181 ccgaccgatc cccggattct ggtcgacgcc gcgacccggg acgacgctgc cgtgtttcag
      241 gtggcgccgg accgtgcgct ggtggcgacc gtggacttct tcacgcccat cgtcgacgac
      301 gcctacgact tcggccgcat cgccgccgcc aacgcgttct ccgacgtata cgcgatgggg
      361 gcggtgccgc tcttcgccct gaacgtcgtc ggctggccgc gcgagcgcct gccgctcgag
      421 ctgctgggag acgtgctgcg cggcggggcg gacgtagcgc gtgcggcggg agcgttcgtc
      481 gtgggcgggc attcggtgga cgaccccgag cccaagtacg gcatggtcgt gatcggcgag
      541 gtacatccgg agcgcatggt cacgatcgcc ggcgcgcgcc ccggggacgt gctcgtcctc
      601 accaaaccga tcggcacggg aatcctcacg accgccctga agcgcgatct gctcccggca
      661 tccgccctcg tggaggcggt cgaggccatg actaccctga atgccggcgc gtcccgcgcc
      721 atgctcgcca cgggcgttca tgccgcgacc gacgtcaccg ggttcggcct gctgggtcac
      781 ctgcgaaaca tgctggacgc gagcggcgtg gcggccgagg tggaggcagc gcgggtcccc
      841 ctgctgccgc acacccggga gatgctcgcc aagggtgcgg ttccgggagg cagcagccgc
      901 aacctcgaga gcgtggcggc ggtggtgcgg ttcccggagg gcgccaccaa ggcggaccgg
      961 ctgttgctcg ccgacgcgca gacctccggc ggcttgctga tctccctggc cgccgagcga
     1021 gtgccgcggc tgcaggaggc gctgcagcgt gaggggcagg ctccggcggc ggtcatcggc
     1081 cgggtggtcg ccgggcccga gcaagcagga tcgatcgtcg tccgttaaat ctccctcatg
     1141 ggcgtcatcc ctgaataccg gtcactcacg cccgacgcgc tggatgccct ggagcgggcc
     1201 gtgcgcgagc ggcgccgggt ggcgttgtcc cgccgcggca cggagtacgt cgtcgtggcc
     1261 gagcggctcg acgtcgccga ccgggacgag acgctgcagg cacgcctgcc catgaccggg
     1321 gagctgctga gcttccggtt gcgcgatctc gaaaccttcg cggtgctgcc gtgagcatcc
     1381 cccccgcaga caccgccgcc tgatgttcac ccgcttgctg gtgggtctgg atggaagtcc
     1441 gcacggcgat gcggcgctcg agcaggcggt cgtcctggcg cggcgcttcc gctcggtgat
     1501 cgtcgtcgcg gtcgtagcgg aggacgacgc ggccggcgcc acggtgctgc tcgagcgtgg
     1561 gaggttccgc gtccaggccg cgggcctcga ggtcgaagcg gtggagcggc agggctcccc
     1621 cgacgtgaac ctggcggagc tcgccaagga agtcgatgcc gcgctggtcg gacggcgggg
     1681 gcttcacacc gagcgggaag ggccgagcgc catcggccac accgcggcgt cgctgatccg
     1741 gctcgccgag cgcaccgtga tcgtgtgcgg gggcgtggcg tccccgatgc gctcctgtgc
     1801 cgtggcattc gacggccgca accgtcacgc gctcgagctc gccgcgcggt tcgcgagcgt
     1861 cgccgggagc acggtgcacg tgattcacgc ctaccgcgac cgggacgagg gcaccctggt
     1921 cgcgggccag gcggaagcgg agctatcgct gctgggcgtg caattccaga ctcacatcgc
     1981 cgaggggtcg gcgggcgaag tgatcgcccg gctggtccgt gaggtccacg ccgatgcgct
     2041 gttcgcggga gcgcacgtgg cgcgcggcct gggcgagcgg cggccgagcg tcgcgtcgca
     2101 caccgaggag atcctgctgc ataccgatct cccggtcgct attcagccct gatggcgacg
     2161 gccgcgattc cggtcc
//
LOCUS       DBNGZW010000344         4034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1531940, whole genome shotgun sequence.
ACCESSION   DBNGZW010000344 DBNGZW010000000
VERSION     DBNGZW010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1531940"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            284..2152
                     /gene="mnmG"
                     /locus_tag="AC16JC_08160"
     CDS             284..2152
                     /gene="mnmG"
                     /locus_tag="AC16JC_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012845237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HIR5164706.1"
                     /translation="MNSEYDVIVVGGGHAGTEAAGLAARLKARVLLLTANLDTIGQMS
                     CNPAIGGIAKGTVVREVDALGGIMGRAADLATIQFRMLNRSKGPAVWAPRAQCDRTLY
                     RRAVRQLLERLDHLELAQGMVARILVERGCANGVEMTDGRRFLARAIVITAGTFLRGR
                     IHLGTDTQIPAGRAGDQPSVEMAQDIETLGLTVSRFKTGTPPRVDGRSVDWSKVDRQD
                     GEGEGFRFSFYERTELPRQVPCWITWAGEEVKQIIHAHLRESALYGGAISGRGPRYCP
                     SIEDKIVKFPGAARHQIFLEPEGLDTAELYVNGLSTSLPVSVQLEFLRAVPGLERARM
                     TRPGYAIEYDYFPPDQLTPWLETKAVERLFFAGQINGTTGYEEAAGQGVVAGINAVRR
                     LRGLEPVVLGRGDAYIGVLVDDLVTRGVDEPYRLFTSRAEFRLLLRQDNALRRLYPLA
                     ARLGLLDDAERRVAESRLEEEERVLEVARSTSITPPQAAAALAAAGGALAAAERIATL
                     ARRPHVSLALLLRGARVSGGFSEAAVLAAEIELKYDGYLAREREAAERLAELASFTLP
                     PDLRYLELSSLSTESRQKLDRIRPASLAQASRIPGVSPSDLHNLVMEALRWRRCVA"
     gene            2302..3135
                     /locus_tag="AC16JC_08165"
     CDS             2302..3135
                     /locus_tag="AC16JC_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR5164707.1"
                     /translation="MPSRVVAVANQKGGVGKTTTAVNLAASLATAERKTLLIDADPQG
                     NATSGVGIDRVSLKRSLYDALLEGVPLADARVRAVQFPYLDVVPATQDLVGAELELVN
                     RSGRETALRKALRDLRDEYQFVIVDCPPSLGLLTLNVLTAADGVLIPIQCEYYALEGL
                     SQLLNTIKLVQRNFNPALAIDGVLLTMYDARLNLSRQVAEEAKEYFGAGMFAVTIPRN
                     VRLAEAPSFGKPILHYDVQSVGAKSYLAVAQELIRRVERGGAPSPAPALPATAEPEVP
                     A"
     gene            3132..4025
                     /locus_tag="AC16JC_08170"
     CDS             3132..4025
                     /locus_tag="AC16JC_08170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR5164708.1"
                     /translation="MTETAAGRRRLGRGLEALLGPTREEAEREGSLVELPIQDIRPNP
                     LQPRRDVDPTALEELKSSIRQAGLLQPVVVRPAPGGHGVFELIAGERRLRACQALGWE
                     KIPAVRRDVDDRTVLTLALIENLQRDDLSPVDEARGYERLIAEFELTQQDVADAVGRD
                     RSTVTNAMRLLKLPDAVLQMLHDAQLSVGHARALLALDDPRTATTLAREAVAQGLSVR
                     DVEDRVRGGRAPERRPRLKRGLPGAPEVRRVEDALRRRLGTDVRVSLRAKGKGQLHVS
                     FYSNDDLARILELILGAPFDG"
BASE COUNT          647 a         1352 c         1372 g          663 t
ORIGIN      
        1 cctacatcga gggctggtac aatcggcggc gccggcactc gacgctggac tacctgagcc
       61 ccgccgagta tgaactgcgg ctccgtgccg cctaactcag gtgtccatca aaccggggca
      121 acatcagacc aacgcttccg atctcattgc agggccagga ccgactatgt ccgggccgtg
      181 cggcccccgc gtcctcgcgt cctcgcccct accccccggc gtcgcccact tatgcagagc
      241 ttccctaggc gtctgggcgt cgcctggcct atcttgtccc gtcatgaatt ccgagtacga
      301 cgtcatcgtt gtcggtggag gccatgcggg caccgaagcg gcgggccttg cggcgcggct
      361 caaggcccgc gtcctgctcc ttaccgccaa tctcgacacc atcggccaga tgtcctgcaa
      421 tcctgccatc ggtgggatcg ccaagggcac cgtcgtgcgc gaggtggatg ccctgggcgg
      481 catcatgggg cgcgccgccg acctcgccac gatccagttc cgcatgctca accgatcgaa
      541 gggcccggcc gtttgggctc cgcgcgcgca gtgcgaccgc acgctctatc gccgcgcggt
      601 gcggcagttg ctggagcgac tggatcacct cgagctcgcc cagggcatgg tggcccggat
      661 actggtagaa agaggctgcg ccaacggtgt cgagatgacc gacggccgcc ggttcctggc
      721 gcgcgcgatc gtgatcaccg ccggcacctt cctgcgcggc cggatccatc tggggaccga
      781 cacgcagatc ccggctgggc gcgcgggcga tcagccgtcg gtagagatgg cgcaagacat
      841 tgagacactt ggacttacgg tgtcacgctt caagaccggt acgccaccgc gcgtggatgg
      901 ccggtcggtc gactggtcga aggtcgatcg gcaggacggc gagggggagg gcttccgatt
      961 ctccttctac gagcgaaccg aactgccccg ccaggtaccc tgctggatta cctgggcagg
     1021 tgaggaggtg aagcagatca ttcacgctca cctgcgcgag tcggcactgt acggcggcgc
     1081 gatcagtgga cgtgggcccc gctactgtcc gtccatcgaa gacaagatcg tcaagtttcc
     1141 gggagcggcg cgccaccaga ttttcctgga gcccgagggg ttggacacag cggagctgta
     1201 cgtgaacggg ctctccactt ccctgcccgt ctcggtccag ctcgagttcc tgcgcgccgt
     1261 gcccgggctc gagcgcgccc ggatgacgcg tcccggatac gcgatcgaat acgactactt
     1321 cccgccggac cagctgacgc cctggctcga gacgaaggcg gtcgagcggc tgttcttcgc
     1381 cgggcagatc aacgggacga ccgggtacga agaagccgcc ggccaggggg tcgtggccgg
     1441 gatcaacgcc gtgcgccgat tgcgcggtct cgagccggtc gtcctcggac gcggcgatgc
     1501 gtatatcggt gtgctggtag acgacctggt cacgcggggc gtggacgagc cataccgtct
     1561 gttcacctcc agggcggaat tccgattgct gctccggcag gacaacgccc tgcggagact
     1621 gtatccgctc gcggcgcgcc tcggtctgct cgacgacgcc gagcggcggg tggcggagtc
     1681 gcggctcgag gaagaggagc gcgtcctgga ggtcgcgcgg agcacctcga tcaccccacc
     1741 gcaggcggct gcggcgctgg ccgctgcagg cggcgcgctc gcggcagcgg agcgtatcgc
     1801 gacgctcgcc cggcgaccgc acgtatcact ggcgctgctg ctgcgcgggg cgcgggtgag
     1861 cggcggtttc agcgaagcag cggtgctggc cgccgagatc gagctgaaat acgatggcta
     1921 tctcgcgcgg gagcgcgagg cggccgagcg gttggcagag ctcgcgtcgt tcaccctgcc
     1981 tccagacctg cgctacctcg agctgtcgag tctgtcgacc gagtcgagac agaagctcga
     2041 tcggattcga ccggcgtcgc ttgcacaggc atcacgcatc cccggagtgt cgcccagcga
     2101 tctccataac ctggtgatgg aggcgctacg gtggcgtcgg tgcgtcgcct gatcccgggt
     2161 gtttcacgtg aaacggcgtc cggcgaccag tttcacgtga aacgctgggc ccttctttcc
     2221 cccctggcag caacgcagta gattaggtga caacacgtta cgtgtgtagc aagctaacta
     2281 tttatcaaat aacgagttac cgtgccttct cgcgtcgtcg ccgtcgcgaa ccaaaagggt
     2341 ggagtgggca agacgaccac cgccgtgaac ctggcggctt cgcttgccac ggccgagcgc
     2401 aagacgctgc tgatcgatgc cgatccccag ggcaacgcca cgagcggcgt cggcatcgat
     2461 cgcgtgtcgc tgaagcgctc gctctacgat gcattgctcg agggcgtgcc gctcgccgac
     2521 gcgcgcgtgc gcgccgtgca gttcccctat ctcgacgtcg ttcccgcgac gcaggatctg
     2581 gtcggcgccg agctggagct cgtgaaccgc tcgggccgcg agacggcgct gcgcaaggcg
     2641 ctgcgcgacc tgcgcgacga ataccagttc gtcatcgtcg attgtccacc gtcgctcgga
     2701 ctgctgaccc tcaacgtcct caccgccgcc gacggcgtgc tgattccgat tcagtgtgag
     2761 tattacgcgc tggagggact ctcccagctg ctcaacacca tcaagctggt gcagcgaaat
     2821 ttcaatccgg cgctcgccat cgacggtgtg ctgctgacca tgtacgacgc gcgcctcaat
     2881 ctctcgcggc aggtggcgga agaagccaag gagtatttcg gcgccggcat gttcgccgtg
     2941 acgattcccc gcaatgtgcg gctggccgaa gcgcccagtt tcggcaagcc gatcctgcac
     3001 tacgacgtgc aatcggtcgg tgcgaagagt tatctcgccg tggcccagga gctgatccga
     3061 cgggtggagc gcgggggagc accctccccc gcccctgccc tgcctgcgac cgccgaaccg
     3121 gaggtacccg catgacggag acagccgcag gacgacgccg gctcggccgg gggctcgaag
     3181 ccctgctcgg tcccacgcgc gaggaagccg agcgcgaagg cagtctggtc gagctgccga
     3241 ttcaggacat ccgccccaat ccgctgcagc cgcggcgcga tgtggacccc accgcgctcg
     3301 aggagctcaa gtcctcgatc aggcaggcgg gattgctgca gcccgtggtc gtgcgcccgg
     3361 cgcccggcgg gcatggagtg ttcgagctga tcgcgggtga gcggcggctg cgcgcatgcc
     3421 aggcactggg gtgggagaag atccccgccg tgcggcgcga cgtcgatgac cgtaccgtgc
     3481 tgacgctcgc gctgatcgag aacctgcagc gggatgacct gtccccggtg gatgaagcgc
     3541 gcgggtacga gcggctcatc gcggaattcg agttgacgca gcaagacgtc gccgacgcgg
     3601 tgggtcgcga tcgctccacc gtgacgaacg ccatgcgcct gctgaagctc cccgacgccg
     3661 tgctgcagat gctgcacgac gcgcagctct ccgtcggtca cgcgcgcgcc ctactggccc
     3721 tcgacgaccc ccggaccgcg accaccctcg cgcgcgaagc cgtggcccag ggtctgtcgg
     3781 tgcgcgacgt ggaggaccgc gtgcgggggg ggcgcgcgcc ggagcgtcgg ccgcgactca
     3841 agcgcgggtt gcccggggct cccgaggtac ggcgcgtgga ggacgcgttg cgccggcgcc
     3901 tgggcaccga tgtccgggtg tcgttgcgcg cgaaagggaa ggggcaactc cacgtctcct
     3961 tctattcgaa cgacgatctc gcccgcattc tcgagctgat tctcggagct cccttcgacg
     4021 gatgaagcca cgca
//
LOCUS       DBNGZW010000345         4438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1600793, whole genome shotgun sequence.
ACCESSION   DBNGZW010000345 DBNGZW010000000
VERSION     DBNGZW010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4438
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1600793"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="AC16JC_08175"
     CDS             <1..210
                     /locus_tag="AC16JC_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164709.1"
                     /translation="ERANATISVATFSGSFDACFPVTIPPGGRTTKHRFTFTLGTGSA
                     RVELESFDGSIRLCRPGQTHERNEE"
     gene            216..1001
                     /locus_tag="AC16JC_08180"
     CDS             216..1001
                     /locus_tag="AC16JC_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR5164710.1"
                     /translation="MRATVFQIPAILATALLAAAPVGAQDFHWTGKLAAGKRLEIRGV
                     NGSIRTVAATGNEIDVSARKTAHRSDPDEVKIEVVPSDEGVTICAVYPTPSRARRDNT
                     CEPGDGWHSSTENNDVTVDFMIKLPAGIVFSGETVNGEIDAEGLGADAEVSTVNGSID
                     VEATGHVEARTVNGSIRATMGRADWPSQAEFTTVNGGITLTLPGNLSAEVSAETVNGD
                     LETDFPLTVTGKFGPRHMRGTIGNSGRYLKLTTVNGSIGLRKA"
     gene            1142..4168
                     /locus_tag="AC16JC_08185"
     CDS             1142..4168
                     /locus_tag="AC16JC_08185"
                     /inference="COORDINATES: protein motif:HMM:NF019291.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HIR5164711.1"
                     /translation="MLRELLTRTEQISDLRDLFRVLGYQAAWETVPPGPWLGSAVVDA
                     ARITGAALIARHDAFRVIALAAGDPEAAARAGARRLAAAADRGLVCALGGAPRRLVVA
                     SWRSTATPQLAVRLASTQLERPAAVALATLERLATRPGETALALSLRVGEALASEAVT
                     TRFFHAYRSLVERFAAQLSAPRNMADRHTLALTTLTRVLFLYFVQEKGWLDGDRRYLP
                     ALLERAVARGRHFHRAALHPLCFGALNRAPVDRSTTVRRLGRIPFLNGGLFEPTALEQ
                     AHGPAVWGNADWRDAFDEVFERFHFSVRESDADDRVAPDMLGRVFEGVMEPVERRASG
                     SYYTPASLVRELVRAALSAALVHRAGLPRGHAERWVYGGVPPAKAPDLRRLTLLDPAA
                     GSGAFLLGALEEIARLRSTAGEHDTPALRREIIAHSLYGVDLSPTAVRLAELRLWLAL
                     VAGDATTDLSAVTPLPNLDGQLRCGDALLDPYTVAASLANARPPTGVSRNTARLAQRR
                     RELFALSGRAKRIAALELTRAESTLAEGLYDGALVALERAIRELLGVARTRDLFGHRT
                     RLDVTQRARLRRLRAARATLRSARRRLVREGGTPFFSVESHFGDLIAAGGFDVVLGNP
                     PWVRGERLPARVREVLSQRYSTWRSGVGPFAHIPDLSVAFCERALELLAPGGVVALLT
                     PAKLATAGYAETLRRRLAGETRLEHVAPLDESAAASFGAAVYPMALVAARRHPEPGAT
                     CGRTLDPSESDRVPQQLLQAPGAWVLVPDGAAVAGRLARENRRLAERWPPLLGVKTGA
                     DALFLAVEPLEGARPAVRGRDVARWQVDPRVFVLWTHDVHGRPLERLPARMAERLLPH
                     AERLRRRADYRGGPPWQLFRTRLGVAPHRVVWADLARELGAAVAPPEVVPLNTVYGIA
                     TASDEDAHALAALLNTRWCTALARLAADPARGGFRRFNASVVGRLPLPDVDAEVWRAL
                     AEHGRRREPADALVSDLYGLDETDRNALGKLAPHPR"
     gene            4134..>4438
                     /locus_tag="AC16JC_08190"
     CDS             4134..>4438
                     /locus_tag="AC16JC_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164712.1"
                     /translation="MRSASSPLILAESLRPLEEPLALLVTAGPEVSPPTACRAAALAL
                     LDLPPRSSPAPDWLRPHQVPAHTRLLAIVDRFGGAVLADAVGLGKSYIALAVAQSLGA
                     "
BASE COUNT          600 a         1632 c         1543 g          663 t
ORIGIN      
        1 gaacgggcca acgccacgat ctcggtggcc acgttcagcg gcagtttcga tgcgtgtttc
       61 ccggtgacga tcccccccgg ggggaggacg acgaaacacc ggttcacctt cacccttggc
      121 acggggagcg cccgggtgga gctcgaatcc ttcgacggct cgatcaggtt gtgccggccc
      181 gggcagaccc acgaacggaa cgaggagtga gcgacatgcg cgcgacagtc ttccagatcc
      241 cggcgatcct tgccacggcg ctgctggcgg cggcaccggt gggcgcccag gacttccact
      301 ggaccggcaa gctggccgcc ggcaagcggc tcgagatcag gggtgtgaac ggcagcatcc
      361 gcaccgtggc cgccacgggc aacgagatcg acgtgagcgc ccgcaagacg gcgcatcgct
      421 ccgatcccga cgaggtgaag atcgaggtcg ttccctccga cgagggcgtc acgatctgcg
      481 ccgtgtaccc cacaccctcc cgggcgcggc gcgacaacac ctgtgagccg ggcgacgggt
      541 ggcactcctc caccgagaac aacgacgtca ccgtcgactt catgatcaag ctccccgcgg
      601 gcatcgtgtt cagcggagag accgtcaacg gcgagatcga cgccgaaggg ctgggcgccg
      661 acgccgaggt gagcacggtc aacggcagca tcgacgtgga agcgacgggc cacgtcgagg
      721 cccggactgt gaacggatcg atccgcgcca ccatggggcg ggccgattgg ccgtcgcagg
      781 cggagttcac cacggtgaac ggcggcatta cgctgaccct gcccggcaac ctcagcgccg
      841 aggtgagcgc cgagaccgtg aacggcgacc tcgagaccga cttcccgctc acggtgacgg
      901 gcaagttcgg cccccgacat atgcgcggca ccatcgggaa cagcggccgc tatctcaaac
      961 tgacgacggt gaacgggagt attggtttga gaaaagcatg aaagcaggga gcagggagca
     1021 cggagcagtc tgctctgcgc tccctgctcc cgcagttttt cggtctctcc ggcagtaatc
     1081 cggtacctca ccgtcgacca tcccgtctca tcttgggccc cttcgaaccg aggggcccgc
     1141 ggtgcttcgg gagcttctca cccgcaccga gcagatctcc gatcttcgcg acctgtttcg
     1201 cgtgctcggc tatcaggcgg cgtgggagac cgtgccaccc gggccctggc tcggttccgc
     1261 tgtcgtcgac gccgcccgta ttaccggggc cgcgctcatc gcacgacatg acgcctttcg
     1321 cgtcatcgcg ctggccgctg gcgacccgga agcggccgcc cgtgccggcg cccggcgtct
     1381 cgccgccgcc gccgatcgcg gcctggtgtg cgcgctgggc ggcgcaccgc gccgcctggt
     1441 cgtcgcctcc tggcggtcca cggcgacacc ccagctggcc gtccgtctcg cgtccactca
     1501 gctcgagcga ccggcggcgg tcgcgctcgc caccctcgag cgcctcgcca cgcgccctgg
     1561 agagacggcg ctggccctgt cgctgcgggt gggtgaagct ctcgccagcg aggccgtcac
     1621 cacccggttc ttccacgcat accggtccct ggtcgagcga ttcgccgcgc agctgagcgc
     1681 gccacgcaac atggcggatc gccacaccct cgcgctcacc accctcaccc gggtgttgtt
     1741 cctctacttc gtgcaggaga agggctggtt ggacggcgac cggcggtacc tgccggcgct
     1801 gctggaacgc gccgtcgcgc gaggacgtca cttccatcgg gccgccctcc atccgctctg
     1861 cttcggcgcc ttgaatcgcg cgccggtcga ccgctcgacg accgtccgga gactggggcg
     1921 gattccgttc ctcaacggcg ggctgttcga gcccaccgcg ctcgaacaag cccacggacc
     1981 ggccgtatgg gggaacgccg actggcgcga cgcgttcgac gaggtgttcg agcgattcca
     2041 cttctccgtg cgcgagagcg atgcggacga ccgcgtcgcc ccggacatgc tcggacgggt
     2101 gttcgaaggc gtgatggagc cggtcgagcg gcgtgccagc ggcagttact acacacccgc
     2161 ctccctggtg cgggagctcg tgcgtgccgc gttgtcggcc gcgctggtgc accgtgcggg
     2221 tctgccgcgc gggcacgccg agcgatgggt gtacggcggg gttccccccg cgaaggcgcc
     2281 cgacctccgt cgactgacgc tgctcgaccc tgccgccggg tcgggagcgt tcctgctcgg
     2341 cgcgctcgag gagatcgcgc ggctgcgctc gacggccggg gagcacgaca cccccgcgct
     2401 gcggcgcgag atcatcgccc attcgctcta cggggtggac ctttccccta ccgccgtccg
     2461 gctcgccgag ttgcggctgt ggctggcgct cgtcgccggg gacgccacca ccgacctctc
     2521 cgcggtgacg ccgttgccga acctggatgg ccagctgcgt tgcggggacg cgctcctcga
     2581 cccctacacc gtcgccgctt cgctcgccaa tgcccgcccg ccgaccggcg tctcgcgcaa
     2641 caccgcccgc ttggcccagc ggcgccggga gctgttcgcg ctctccggca gagccaagcg
     2701 aatcgccgcg ctcgagctca ctcgcgccga aagcaccctg gcggaaggac tgtacgacgg
     2761 ggcgctcgtt gcactggagc gtgcaatccg tgagctgctg ggcgtggccc gtacccgcga
     2821 cctgttcggt catcgcaccc gactcgatgt gacgcaacga gcccggttgc gccgcctgcg
     2881 cgcagcgcgc gcgacgctgc gctccgcccg gagaagactc gtccgcgagg gcggcacgcc
     2941 cttcttcagc gtggaatcgc acttcggcga cctgatcgcc gcgggcgggt tcgacgtggt
     3001 gctcggcaat ccgccctggg tgcgaggcga gcggctgccc gcgagggtcc gcgaagtact
     3061 ctcgcagcgc tactcgacct ggcgctcggg cgttggtccg ttcgcccata tccccgatct
     3121 ctcggtggcg ttctgcgagc gcgcgctgga gctcctggcc ccgggaggcg tcgtggcact
     3181 gctgacgccc gccaagctcg ccacggccgg atacgccgaa accctgcgcc ggcgtctggc
     3241 gggcgaaacc cggctcgagc atgtggctcc gttggacgag tccgcggctg cctccttcgg
     3301 cgccgcggtc tatcccatgg ccctggtcgc cgcccgccgc caccccgaac cgggcgccac
     3361 ctgcggcaga acgctggatc cctccgagag cgaccgggtt ccgcaacagc tgctgcaggc
     3421 acccggcgcc tgggtgctgg tgccggacgg ggccgccgtc gccggacggc tggcgcggga
     3481 aaaccgccgg ttggcggagc ggtggccgcc cctgctcggc gtgaagacgg gagccgatgc
     3541 cctcttcctc gcagtcgagc cgctcgaggg cgccagaccg gcggtgcgcg gccgggacgt
     3601 tgcccgctgg caggtggacc ccagggtctt cgtgctctgg acccacgatg tccacggccg
     3661 gccgctcgag cggctgccgg cacgcatggc cgagcgactg ctgccgcatg ccgagcgtct
     3721 gcgtcgccgg gcggactatc ggggcgggcc gccgtggcag ctgtttcgca cccggctggg
     3781 ggtcgcgccg caccgcgtcg tgtgggccga cctggcccgc gagctgggag cggccgtggc
     3841 gcccccggaa gtcgtgccgc tcaacaccgt gtacggcatc gccacggctt ccgacgagga
     3901 cgcgcatgcg ctcgccgcgt tgctgaacac gcgctggtgt accgcgctgg cccggctggc
     3961 cgccgatccg gcgcgcggcg ggttccggcg cttcaacgcg agcgtggtcg gacgcctgcc
     4021 gttgccggac gtggacgccg aggtctggcg ggcgctggcc gagcacggcc ggcgtcgcga
     4081 gccggccgac gcactcgtgt ccgacctgta cgggctcgat gagaccgatc gcaatgcgct
     4141 cggcaagctc gcccctcatc ctcgctgaat cgctccggcc gctcgaggag ccgctcgcgc
     4201 tgctcgtcac ggccggaccg gaggtgtcgc caccgacggc gtgccgggcg gcggcccttg
     4261 cactgctcga cctgccacca cgttcgagtc cggcacccga ctggctccgg ccgcaccagg
     4321 tccccgccca cacccggttg ctcgccatcg tggaccggtt cggcggtgcg gtgctggcgg
     4381 acgcggtggg cctgggcaag tcctacatcg cactggccgt cgcccaatcg ctcggcgc
//
LOCUS       DBNGZW010000346         6209 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1532473, whole genome shotgun sequence.
ACCESSION   DBNGZW010000346 DBNGZW010000000
VERSION     DBNGZW010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1532473"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1202)
                     /locus_tag="AC16JC_08195"
     CDS             complement(<1..1202)
                     /locus_tag="AC16JC_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HIR5164713.1"
                     /translation="MTVALLLAGGSGVLRAQQSHDFELSVFGAYTRYDKAFNLDGGIG
                     GGARLTYFLTRSVGFGADVLFQREQDVPGSAGATMDPMIASANLVVRLPTLLYAVAGY
                     SRLDFGASSPFSFTDGGLHGGLGVRLPLGERVGLMVEGRAIYTPNTKGAFGSDHATHL
                     LALAGFSFLQGGGTRAGPKDADADGVIDKRDACPRTPAGATVDVRGCPSDSDQDAVLD
                     GLDKCPGTPAGAKIDATGCPLDADRDGVADGLDQCPDTPAGATVNNVGCPSDTDRDGV
                     LDGLDRCPDSPVGATVDAQGCPSDADGDKVVDGVDRCPNTPPGVIVDAAGCPKDGDND
                     GVPDGIDQCPNSPVGQRVDATGCPLARDSDGDGVADNLDRCPGTPPGTPVDQVGCVVL
                     FREERAPGA"
     gene            complement(1278..2645)
                     /locus_tag="AC16JC_08200"
     CDS             complement(1278..2645)
                     /locus_tag="AC16JC_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR5164714.1"
                     /translation="MGILRTPARVSHALTIAVLVTAVPAFAQRGSFEAGAFGALESFS
                     PRFDLRMGIAGGGRLGFSPRPGWTLELELGAGSATIAGGGRSIPLTSIGLHALRGLGV
                     DDRGWFALAGYARPIFHGTPPGRFSDDAIALGLGHRAGLGARLALRTELRGQYAFSST
                     LDGRGAGQLLALVGLSFLPKAHSAADLDADGIPDARDACPRTPDGAIVDRRGCPADSD
                     GDGHLDGLDRCPNTPPGVVTEATGCPVDTDRDGIYDGADQCPGSPVGSVVDARGCPLD
                     ADGDGVGDADDRCPGTPPHAPVNTAGCPVSHDADGDGVDDARDRCPGTTPGTPVDPLG
                     CQVLFARERESLVLKGVNFETGSARLLPPSYVVLDQVAASLLAHADVRIEIAGYTDNT
                     GSARTNTRLSAERARTVQDYLHQRGVTTERMRAAGYGPADPVASNTTPAGRARNRRVE
                     LRQIP"
     gene            complement(2633..4078)
                     /locus_tag="AC16JC_08205"
     CDS             complement(2633..4078)
                     /locus_tag="AC16JC_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Kelch repeat-containing protein"
                     /protein_id="HIR5164715.1"
                     /translation="MAPRGASVALGALAAALACGGPSTGPAPFRPRIDYVDGAIEPVL
                     VNGQAMVLEGFGFGDVQGNGAIQFARAGGGIVPAVVVPGGWSDRTVRAIVPDDAVSGA
                     LTLTTNAGRTLSVVVHVVAHVAFDPATLTWQARTAFPRAPVGVGLAVGSEAGSGGLRV
                     VLYAAGGAETETGTGKVSPDGAVFVARAAPGGAIGVWRQTPDLPAKRAFAAVAFANRY
                     NSRYAKRALYVIGGVDDAGRARATVYRTLPDADTAVGAFTTIEPLPAPVAGAIAVVRR
                     GRIYVIGGADSLGRPQTNVFVGRIGLDGHIDGWYTQPALPGPRAYGGGVVLDDRATVF
                     GGIGDSAEPGVGLDTLLPRLAAGDGAPVSLLSGFFTGTWAPGGPLLPAARSQFATLTL
                     GDVILLVGGVYAGATANTSETIAATVFARDSLGDFSGPVGTNSIAGLGGGTLVSAAGA
                     SWLDWDGTPHGLVLGGIDLVTRDRRAGVWGF"
     gene            complement(4059..>6209)
                     /locus_tag="AC16JC_08210"
     CDS             complement(4059..>6209)
                     /locus_tag="AC16JC_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR5164716.1"
                     /translation="SLAQDAAAGMSPLPRSRSMPATIVPVAVALGALAAIGVAFAGYA
                     SARLATSGSFALALTVLVVIGALTRRYGIPLPGNGFSSYVLGVTTFAVLERGWEFAAV
                     VAPLLTVLGDVVLRRLPLRIAGSNAAHLTAGSALVGIIYERLGGGTGSLALEPDNIGP
                     IAALIVLMMVIVNGSFYLELAMTRSVAWVDARLTARWETIVFATSVGFALLWFRLFHT
                     ALPIAAWILVAAGLLGATLVSGHVIRLGVHADELLLVQRLARTIVTDLNLTRTFAHIQ
                     ETTRRLVPWEHIGFARFDARRREMELIADTAMQDGVNAAFRFDADAGLSGEAVRTRRP
                     VVARNLGRGDALTPGSERTGSEILVPLFHGEQLIGIWSIRHSDPWMYRESDGDILSLA
                     APQIALMLALETAVRPVVGASDQTTQYVQTITATTQEIHASSQEVAASAQRASQEARR
                     ATHLVAAAAEEAGELRNGAADLATAGDQTREAGDQVERTAARVRTATQEAVRRLSDLG
                     RSTAEGAKEMTRLREAAAQVERFSEAIGFVANQTNLLALNATIEAARAGVHGHGFAVV
                     ADEVHKLAEEAGREARNVGKSVQETLRVLDRGSQLLQRIRTELDGVVENSSALLPDLD
                     RIAEVAGGTARAGKRVAEVARSTADRAARMGQSLAEAQNGAGASAQEAESVAAAAAEQ
                     LRAIEDLANGATELSSIAERLARTVEFIRGAEGR"
BASE COUNT          915 a         2248 c         2192 g          854 t
ORIGIN      
        1 gcgccgggcg cgcgttcttc gcggaacagg acgacgcagc cgacctgatc caccggcgtt
       61 ccgggcggag ttccggggca ccggtcgaga ttatccgcga cgccgtcgcc gtcggagtcc
      121 cgcgcgaggg ggcacccggt ggcgtccaca cgctgcccga cgggagagtt cgggcactgg
      181 tcgatcccat cggggacacc gtcgttgtcg ccgtccttgg gacatccggc cgcgtccacg
      241 atcactccgg gcggcgtgtt ggggcagcgg tccacaccgt cgaccacctt gtcgccgtcc
      301 gcgtcgctgg ggcagccctg cgcgtccacc gtcgccccga cgggggagtc cgggcaccga
      361 tcgagcccgt cgagaacgcc gtcccggtcc gtatcggagg ggcagcccac gttgttgacc
      421 gtggcgccgg ctggagtgtc gggacattgg tccaggccgt cggcgacgcc gtcccgatcc
      481 gcatcgagcg ggcacccggt tgcgtcgatc ttcgcacccg ccggcgtccc cgggcacttg
      541 tcgagaccgt ccagtaccgc gtcctggtcc gaatcgctgg gacagccgcg cacgtccacg
      601 gtcgcgcccg cgggcgtccg gggacacgcg tcgcgtttgt cgatgacgcc gtcggcgtcg
      661 gcgtccttcg gtcccgcgcg cgtgccgccc ccctgcagga acgagaagcc cgcgagggcc
      721 agcaggtgcg tggcgtgatc ggagccgaag gcgcccttgg tgttgggcgt gtagatcgcg
      781 cggccttcca ccatcaggcc gacgcgctcc ccgagcggca ggcgcacccc cagtcccccg
      841 tgcaggccgc cgtcggtgaa ggaaaagggg ctgctcgcgc cgaagtcgag gcgggagtac
      901 ccggcgacgg cgtacagcag ggtcgggagc cggaccacca gattggcgct ggcgatcatg
      961 gggtccatcg tggcgccggc ggagccgggg acatcctgct cgcgctgaaa cagcacgtcg
     1021 gcgccgaagc cgacgctgcg cgtgaggaaa tacgtcaggc gcgcgccgcc gccgattcca
     1081 ccgtccaggt tgaacgcctt gtcgtagcgc gtgtacgctc cgaagacgct cagctcgaaa
     1141 tcgtggctct gttgggcccg gagcactccg ctcccgcccg ccagcagcaa tgcgacggtc
     1201 atgatcgcgc gcaaaatgag cctccctccg atcattccaa gataggaaac agggccacag
     1261 cgcgcgatgc cgctgcctca gggaatctga cggagctcca cgcgacggtt gcgcgctcgc
     1321 cccgccggcg tcgtattgga ggcgacgggg tccgccggcc cgtacccggc ggctcgcatt
     1381 cgctccgtgg tcacaccgcg ctggtggagg tagtcctgaa ccgtacgggc gcgctcggcc
     1441 gacagccgcg tgttggtgcg cgcggaccct gtgttgtcgg tgtacccggc gatctcgatg
     1501 cgcacatcgg catgcgcgag cagcgacgcc gccacctggt cgagtacgac gtacgacggc
     1561 ggcagcaggc gcgcgctccc ggtttcgaag ttcacgccct taagcaccag ggactcgcgc
     1621 tcgcgcgcga acagcacctg acaccccagt gggtcgacgg gcgtgccggg agtcgtcccc
     1681 gggcagcgat cccgggcgtc atcgacgccg tcgccgtcgg cgtcgtggct caccggacac
     1741 cccgcggtgt tcacgggcgc gtgcggcggc gtgccggggc agcggtcgtc tgcgtcaccc
     1801 acgccgtcgc catccgcatc cagcgggcag ccgcgcgcat cgacgaccga gcccacggga
     1861 gagccggggc actgatccgc gccgtcgtag atgccgtcgc ggtcggtatc gacggggcag
     1921 ccggtggcct cggtgacgac tcctggcggg gtgttcggac agcggtccag gccgtcgagg
     1981 tggccgtcgc cgtcggaatc cgccgggcag ccgcgccgat cgacgatcgc tccgtcaggc
     2041 gtccgcgggc acgcgtcgcg tgcgtccggg atgccgtcgg catcgaggtc cgcggccgag
     2101 tgggccttcg gcaggaacga cagtccgacc agggcgagga gctgacccgc cccgcgcccg
     2161 tcgagggtgg aggagaaggc gtactgaccg cgcagctccg ttcggagagc caagcgggct
     2221 ccgagcccgg cgcgatggcc caggccgagg gcgatcgcat cgtcactgaa acgcccgggg
     2281 ggcgtgccat ggaagatggg cctggcgtag ccggcgagtg cgaaccaccc gcggtcgtcg
     2341 acacccaggc cgcgcagcgc atggagtccg atggaggtga gcggaatgga ccggccgcca
     2401 cctgcgatcg ttgcacttcc ggcgcccagc tccagttcga gcgtccagcc cggtcgaggg
     2461 ctgaacccca ggcggccgcc tccggcgatc cccatgcgca gatcgaagcg cggactgaag
     2521 gactccaggg ccccaaatgc gcccgcctcg aaggaccctc gctgggcgaa cgcggggacg
     2581 gccgtgacga gcacggcaat ggtcagcgcg tgactgacgc gcgccggcgt tctcagaatc
     2641 cccacacccc cgcgcgtcgg tcgcgcgtga ccaggtcgat gccgccgagc acgagaccgt
     2701 gtggcgtgcc gtcccagtcc agccagctcg cccccgcggc gctgaccagc gttccgccgc
     2761 ccagtcctgc gatggagttc gtgcccaccg ggccggagaa atcgcccagc gaatctcggg
     2821 cgaacacggt cgccgcgatc gtctccgacg tgttggcggt ggcgccggcg tagacaccgc
     2881 cgacgagcag gatgacgtca cccagcgtca gtgtcgcgaa ctgggaccgt gccgcgggga
     2941 gcaggggtcc acccggtgcc catgtgccgg tgaagaatcc cgagaggaga ctcacggggg
     3001 ccccgtcgcc cgcggcgagg cgcggcaata gcgtatcgag gccgacgccc ggctccgcgc
     3061 tgtcgccgat cccgccgaac accgttgccc ggtcgtcgag cacgacccct ccgccatagg
     3121 cgcgcgggcc gggtaacgcc ggctgcgtgt accacccgtc gatatggcca tccagcccga
     3181 tgcgcccgac gaacacgttg gtctgcggcc gccccaggga gtcggcgcct cctatcacat
     3241 agatccggcc gcgccggacg acggcgatgg ctcccgcaac cggagccggg agcggctcga
     3301 tcgtcgtgaa cgcaccgacc gccgtgtcgg cgtcgggcag tgtccgatag accgttgccc
     3361 gggcgcgtcc cgcgtcgtcg acgcccccga tcacgtacaa ggcgcgtttc gcgtagcgcg
     3421 agttgtagcg attggcgaac gcgaccgcgg cgaatgcccg cttcgccggg agatcggggg
     3481 tctggcgcca cacgccgatt gcgcctcccg gcgcggcacg ggcgacgaat accgccccat
     3541 ccggggaaac cttccccgtg ccggtctccg tctcggctcc accggcggca tacaggacca
     3601 cgcggagtcc gccgctcccc gcctcgctgc ccacggcgag ccccacgccc acgggagcac
     3661 ggggaaacgc ggtgcgggcc tgccaggtga gggtcgcggg gtcgaaggcg acgtgggcca
     3721 caacgtggac gacgaccgag agcgttcgtc cggcatttgt cgtgagcgtc agcgccccgc
     3781 tcaccgcgtc atcggggacg atcgctcgta ccgtgcgatc gctccagcct cctggcacga
     3841 ccacggcggg cactatgcct ccgccggccc gtgcgaactg aatcgcgccg ttgccctgga
     3901 cgtcgccgaa accgaatcct tccagcacca tggcctgccc gttcaccagc acgggttcga
     3961 tcgcgccgtc gacgtagtcg atccgcggcc tgaacggcgc cggcccggtg gacggaccgc
     4021 cgcaggccag cgcagccgca agcgccccga gggcgacgct agcgcccctc ggcgccacgg
     4081 atgaactcca ccgtccgggc gagtcgctcg gcgatgctgg acagctcggt cgcgccgttg
     4141 gccagatcct cgatggcgcg cagctgctcg gcggctgccg cggccacgga ctccgcctcc
     4201 tgggccgagg cgccggcccc gttctgagct tcggcaagcg actggcccat gcgggcggcg
     4261 cggtccgcgg tgctccgggc gacctccgcc acgcgcttgc ccgcccgagc cgtgccgccc
     4321 gcgacttcgg cgatacggtc gaggtcgggg agaagggcgc tggagttctc gacgaccccg
     4381 tccagctccg tgcggatgcg ctggagcagc tggctaccgc gatcgaggac gcgcagcgtc
     4441 tcctgtaccg acttccccac gttgcgcgcc tcgcgccccg cctcttcggc gagcttgtgc
     4501 acttcgtcgg cgactacggc gaacccgtgg ccgtgcacgc cggctcgcgc cgcctcgatc
     4561 gtcgcgttga gggccagcag gttggtctga ttcgccacga atccgatcgc ctcggagaag
     4621 cgctcgacct gggccgctgc ttcgcgcagc cgcgtcatct ccttcgcgcc ctcagcggtg
     4681 gaacgtccca ggtcgctcag ccgacgcacg gcctcctgcg tcgccgtgcg gacccgcgcg
     4741 gcggtccgct cgacctggtc gcccgcctct ctcgtctggt cgccggccgt cgccaggtcg
     4801 gccgccccgt tgcgaagctc gcccgcctcc tccgcggccg cggccaccag atgcgtcgcc
     4861 cggcgtgctt cctggctggc ccgctgggcg gaagcggcca cctcctggct cgaggcgtga
     4921 atctcctgcg tcgtcgcggt gatcgtctgg acgtactgcg tcgtctggtc ggaggcgccg
     4981 accaccgggc gcaccgcggt ctcgagggcc agcatcagcg cgatctgcgg agcagcgagg
     5041 ctcaggatgt caccgtcgga ttcccggtac atccacggat ccgagtgccg gatgctccag
     5101 atgccgatca gctgctctcc gtggaagagc gggaccagaa tctccgaccc ggtgcgctcg
     5161 cttccgggag tgagggcatc gccgcgcccc aggttccgcg cgaccaccgg gcgccgggtg
     5221 cgcacggcct ctcccgacag gcccgcgtcc gcatcgaacc ggaaggcggc attcacaccg
     5281 tcctgcatcg ctgtgtcggc gatcaactcc atttcccgtc gtctcgcgtc gaaccgtgcg
     5341 aatccgatat gctcccaggg gacgagccgg cgggtggttt cctggatgtg agcgaaggtg
     5401 cgcgtcaggt tgaggtcggt cacgatggtg cgcgccagcc gttgcacgag cagcaactcg
     5461 tccgcatgca cgcccaatcg gatcacgtgg cccgagacca gcgtcgcacc caacaacccg
     5521 gccgccacca gaatccacgc cgcgatcggc agggccgtgt gaaacagccg gaaccagagc
     5581 agcgcgaacc ccacggaggt ggcgaagacg atcgtctccc accgggcggt gagtcgggcg
     5641 tccacccacg cgacggagcg ggtcatcgcg agctcgagat agaacgagcc gttgacgatc
     5701 accatcatca ggacgatgag cgccgcgatg ggcccgatgt tgtccggctc gagcgcgaga
     5761 ctgcccgttc cgccgcccag gcgctcgtag atgataccca ccagcgccga gccagcggtg
     5821 aggtgggccg cgttcgagcc ggcgatgcgc agcggcagac ggcggagcac gacgtctccc
     5881 aggacggtga gcaggggcgc gacgacggcc gcgaactccc agccgcgctc cagcacggca
     5941 aaggtggtca cgcccagcac gtaggaggag aaaccattgc ctggcagcgg aatgccgtaa
     6001 cgccgggtga gcgccccgat cacgacgagg acggtgaggg ccagcgcgaa gctcccgctg
     6061 gtggcgagcc gggcactcgc gtatccggcg aacgccacac cgatggcggc gagggcgccg
     6121 agggcgacgg cgaccgggac gatggtcgcg ggcatcgacc gggaacgcgg gagcggactc
     6181 atgccggccg cggcatcctg cgcgagaga
//
LOCUS       DBNGZW010000347         4747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1874793, whole genome shotgun sequence.
ACCESSION   DBNGZW010000347 DBNGZW010000000
VERSION     DBNGZW010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1874793"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /locus_tag="AC16JC_08215"
     CDS             <1..1082
                     /locus_tag="AC16JC_08215"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR5164717.1"
                     /translation="NPIAARVLDVHGMATRRYFVFLPRSGRPVAVAHRIEMAPFEQFP
                     GEVRPYAAWRELRAQLEPLVRGKTLALETSPQDGVPYLDRVPHGVVQLLEALGARVVS
                     SAPLVTRFAARWSDAELAGHRRAAEAIGAIARDALAWAGAELARGAEVRETAVQRRVV
                     DAFDRAGLVTDHPPIAAFGPNAANPHYEPRAGADRRLAAGEVLLLDLWAGVSHDAVFA
                     DQTWMAFAGRRPPDEVARVWGVVRDARDAAVARLAERWGTGVTGADLDDAARGVIAAA
                     GYGEWFVHRTGHSIDRDLHGSGPHLDNFETADTRELLPGIGFSVEPGIYLPGRFGVRS
                     EINVHLGDAGPEVTPAAPQRELQIV"
     gene            1423..4734
                     /locus_tag="AC16JC_08220"
     CDS             1423..4734
                     /locus_tag="AC16JC_08220"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR5164718.1"
                     /translation="MTHRVRSAFGRFLALGAVVALATFVAVAPAAAQQTTGKIEGTVS
                     DQAGVALANAQVFVVGTSFGAVTNEKGYYFINNVPVGSFTLRAQFIGYAPTEVRDVRV
                     FGGQTITQDIKLSPSAIQVSGLTVTAAANPIVPRDQVASKSIVAGDVVNSLPVDDVRD
                     VIALQPGVVESGSGAGVSIRGGRPGEANVYIDGTPVRGTNSGSQRITLGTNAVEEATV
                     TTGALGVEFSDAQSGVISYTTRAGGERLRSSLSYETDQPFGNSISAGYTRLEGSLGGP
                     LIGNLRFFGSAVLQGQQTDFRSLGADEVPFFATAGVDQVVVDSAEGTSRALPRFVQYS
                     GSCGSLGSSADAVSKAIHDNYGFKCQGARLPMAWRDNLQAQGKLSYSYGSGSSISLTG
                     LAQGQQNRDFPGTAIGGPSLYTGTHAWQRLGVLNVTQSVFKTSERALAINANLSWAQD
                     RSIFGPLDPESELATRDPSLGITFKSLQFSSLEGFPFPITDQIIRNIRTNEGLRVPLG
                     NRSDLETAQGGFRDNPYGMIGRWPTSGINTGATLLSETRYNGRLVADWQANRFHRFTL
                     GGDFKKTNLSYWSSSQLRQIFMDAYVAHPIQYGFFAADRLDLGDVVIEVGGRWDYYDS
                     KTLFARTPSRIFTNTASVCANGISTSQDAAACPASAPHYAAWSPNAATDDAAYDSSLA
                     RVFTPSVGHHTLSPRLRVSFPITERTDFRLSYSHQVQTPEFTTLLTGVNNDLSFTNSN
                     DVFGRDLDFGKSILFEFGVRHAFSSDVVLDVSAYNKDKVSDFAARILPFVDPSNPTDT
                     LNVNVLTNRDFGNARGVDTKFDWRVASYLSISAAYTFQVSKNTGSDPFSYLNTFARQV
                     SGLSGDRTPPPEAAQRTNDDRTHNLAGAVSLSFPDDYRKGTTLGTVLRNVSVFATVRV
                     QSGLAYTRLVNNGDGQTAPRLAFGLGGRTASGENLNGSTMPWTKYINMRLTKGLRFGR
                     ADITAFADVRNLFNWKNIIGLFAETGDVVNAQHRQKVLNTEYVQLADEAGQAGALEGD
                     GSTVDLNKSCQQWGKPINCESLRMVEARFGNGDGEYTLAEQQRALNTFYDSFFGAYRF
                     NDTPRNIRIGFDVNF"
BASE COUNT          768 a         1564 c         1641 g          774 t
ORIGIN      
        1 tgaacccgat cgccgcgcgc gtgctcgacg tgcacggcat ggcgacgcgt cggtacttcg
       61 tcttcctgcc gcgctccgga cggccggtcg cggtcgccca ccggatcgag atggctccgt
      121 tcgagcagtt cccgggtgaa gtgcgcccct acgccgcgtg gcgcgagctg cgcgcgcagc
      181 tcgagccgct ggtccggggg aaaacgctgg ccctcgagac gtcgccgcag gacggggtgc
      241 cgtacctcga tcgggtgccg cacggcgtgg tccagctgct cgaagcgctg ggcgcgcgcg
      301 tggtgtcgtc ggcgccgctc gtgacccggt tcgcggcacg ctggtcggac gcggagctcg
      361 ccgggcaccg gcgcgccgcg gaagcgatcg gcgccatcgc ccgcgacgcc ctcgcctggg
      421 cgggagcgga gctggcgcgc ggtgccgagg tgcgggaaac cgcggtgcaa cggcgggtcg
      481 tcgacgcgtt cgaccgggcc ggcctcgtca ccgaccaccc gccgatcgcc gcgttcggcc
      541 ccaacgccgc gaaccctcac tacgagccgc gcgccggcgc ggaccggcgt ctcgccgcgg
      601 gcgaggtcct cctgctggat ctgtgggcgg gcgtgtcgca cgacgcggtg ttcgccgacc
      661 agacgtggat ggccttcgcg ggccggaggc cgcccgacga agtggcccgc gtgtgggggg
      721 tggtgcgcga cgcgcgtgat gccgcggtgg cgcgcctcgc cgagcgctgg ggcacgggcg
      781 taaccggcgc cgacctcgac gacgcggcgc gcggcgtgat tgccgcggcg ggctacgggg
      841 agtggttcgt gcatcgtacc ggccactcca tcgaccgcga cctgcacggc tccggccctc
      901 acctcgacaa tttcgagacg gccgacaccc gcgagctgct gccggggatc gggttttcgg
      961 tggagccggg catctacctg ccgggccgct tcggcgtacg cagcgagatc aacgtccatc
     1021 tgggagacgc ggggccggag gtgacaccgg cggctccgca acgcgagctg cagatcgtct
     1081 gaccgcacgt ccgtcacgac atgatggatg catgaattgc ggatgaaaca cgcggcgtgc
     1141 acttgcgtgg cgcatcttct ctccacctga gcctgtccac ttccggggac aaaccccgcc
     1201 tccgggtcgc cttgacagcc cctcgaaacg cgggccactt ttgcggggtc cgcgcgggcc
     1261 accgcgcgcc acttacgcgg ccctgacaga atcctgtcct gcaggccgtc gcccgaggag
     1321 cgtgtgctcg ttcgggagca ctcaccgaga gggaggtgac gtgtcggcaa gcgtttttcc
     1381 gttcggcagt tccagcctca tcacgccatg ggagggtgtg tcatgactca tcgtgtgcga
     1441 agcgccttcg gtcgcttcct cgcgctgggc gcagtggtcg cgctcgcgac gttcgtcgcg
     1501 gtcgccccgg ccgccgcgca gcagacgacg gggaagatcg aaggcacggt gtccgaccaa
     1561 gcgggtgtcg cgcttgcgaa cgcgcaggtc ttcgtcgtgg ggacctcgtt cggcgccgtg
     1621 accaacgaga agggttacta cttcatcaac aacgtgcccg tcggcagctt cacgctgcgc
     1681 gcacaattca tcggctacgc gccgacggaa gtgcgggacg tgcgcgtgtt cggcggccag
     1741 accatcacgc aggacatcaa gctcagcccc tcggcgattc aggtgtccgg gctgacggtg
     1801 acggcggccg cgaacccgat cgtgccgcgg gatcaagtgg cgtcgaaatc gatcgtcgcc
     1861 ggcgacgtgg tcaacagcct gccggtcgat gatgtgcgcg atgtgatcgc gctgcagccg
     1921 ggcgtggtgg agtcgggatc gggcgcgggc gtgtcgatca ggggtgggcg gcccggtgag
     1981 gccaacgtct acatcgatgg caccccggtg cgcggcacca acagtggctc gcagcgcatc
     2041 acgctcggca ccaatgcggt cgaagaagcg acggtgacca ccggagcgct gggggtcgaa
     2101 ttctccgacg cgcagtcggg cgtcatctcc tacacgaccc gcgccggcgg ggaacggctg
     2161 cggagctcgc tctcctacga gaccgatcag ccctttggaa actcgatcag cgccgggtac
     2221 acccgcctcg agggctcgct tggcggaccg ctgatcggca atctgcggtt cttcggctcc
     2281 gccgtgctcc aggggcagca gaccgatttc cggtcgctgg gcgccgacga ggtgccgttc
     2341 ttcgcgacgg cgggcgtgga ccaggttgtg gtggacagcg cggaaggcac gtcccgcgcg
     2401 ctgccgcggt tcgtgcaata cagcggcagc tgcggctcct tggggtcgtc ggccgacgcg
     2461 gtgagcaagg cgatccacga caattacggg ttcaagtgtc agggagcccg gttgccgatg
     2521 gcgtggcgtg acaacctcca ggcccagggc aagctgagct acagctacgg gagcgggtcc
     2581 agcatatcgc tgacgggcct ggcccaggga cagcagaacc gggatttccc cggcacggca
     2641 atcggtgggc cgtcgttgta caccggcacg catgcatggc agcggctggg ggtgctgaac
     2701 gtgacgcaat cggtgttcaa gacgtccgag cgcgccctgg cgatcaacgc caatctgtcg
     2761 tgggcccagg accggtcgat cttcgggccg ctggatccgg agagcgagct cgccacgcgt
     2821 gatccgtcgc tcggcatcac cttcaagtca ctgcaattct cgtcactgga gggtttcccg
     2881 ttcccgatca ccgaccagat catccgcaac atccgcacga acgaagggtt gcgggtcccg
     2941 ctgggcaacc gctccgatct ggagacggcg cagggcggct tccgcgacaa cccctacgga
     3001 atgatcgggc gttggcccac gagcggcatc aacaccggcg cgacgctcct ctccgagacg
     3061 cgctacaacg gccgcctggt ggccgattgg caagccaacc ggtttcaccg cttcacgctg
     3121 ggcggggatt tcaagaagac caatctgtcg tactggagca gctcgcagct gcggcagatc
     3181 ttcatggacg cgtacgtggc gcaccccatc cagtatggct tcttcgccgc ggaccgactc
     3241 gaccttggtg acgtggtgat cgaggtgggc ggtcggtggg actattacga cagcaagacg
     3301 ttgttcgcgc ggacgccgtc gcggatcttc accaacaccg cgtccgtgtg cgccaacggg
     3361 atcagcactt cccaggatgc ggcggcctgc ccggcgagcg cgccgcacta tgcggcctgg
     3421 agccccaacg cggcgacgga cgatgccgcc tatgattcct cgctggcgcg ggtcttcaca
     3481 ccgtcggtgg ggcaccatac gctctcgccg cggctgcgcg tgtccttccc gatcaccgag
     3541 cggaccgatt tccgcctgtc gtattcgcac caggtgcaga cgccggagtt cacgacgctg
     3601 ctgaccggtg tcaacaacga tctgtcgttc accaacagca acgacgtgtt cggtcgcgac
     3661 ctggacttcg gcaagtcgat cctgttcgag ttcggggtgc ggcacgcgtt cagcagcgat
     3721 gtggttctgg acgtgtccgc atacaacaaa gacaaggtgt cggacttcgc cgcccgtatc
     3781 ctgccgtttg tggacccgtc caacccgacc gacacgttga acgtcaacgt gctgacgaat
     3841 cgggacttcg ggaacgcccg cggcgtcgac acgaagttcg actggcgcgt ggcctcgtac
     3901 ctcagcattt ccgcggcgta cacgttccag gtgtcgaaga acaccgggtc ggacccgttc
     3961 tcgtacctga acacgttcgc gcgtcaggtc tccgggctct ccggcgatcg cacgccgccg
     4021 cccgaggcgg cccaacggac caacgacgac cggacgcaca atctggccgg ggccgtgtcg
     4081 ctctcgttcc cggatgacta ccgcaagggg acgacgctcg gcaccgtcct gcgcaatgtg
     4141 agcgtgttcg cgacggtgcg cgtgcagagt gggctggcgt acacccggct ggtgaacaac
     4201 ggcgatggcc agaccgctcc gcggctcgcg tttggcttgg gcggccggac ggcgtcggga
     4261 gagaacctca acggcagcac catgccctgg acgaagtaca tcaacatgcg gctcaccaag
     4321 gggctgcgct tcggccgggc ggacatcacg gcgttcgccg acgtgcgcaa cctgttcaac
     4381 tggaagaaca tcatcggcct gttcgcggaa accggcgacg tggtcaacgc gcagcatcgc
     4441 cagaaggtgc tcaacaccga gtacgtgcag ctggccgacg aagcgggcca ggcgggcgca
     4501 ctggaaggtg acggatcgac cgtggatctg aacaagagct gccagcaatg gggcaaaccg
     4561 atcaactgcg aatcgctgcg catggtggag gcacggttcg gcaatggaga cggggagtac
     4621 acgctggccg aacagcagcg ggcgctcaac acgttctacg attcgttctt tggtgcgtac
     4681 cggttcaatg atacgccccg gaatatccgg attggcttcg acgtgaactt ctaggacatg
     4741 cgttgag
//
LOCUS       DBNGZW010000348         2803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_918291, whole genome shotgun sequence.
ACCESSION   DBNGZW010000348 DBNGZW010000000
VERSION     DBNGZW010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2803
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_918291"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC16JC_08225"
     CDS             <1..291
                     /locus_tag="AC16JC_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164719.1"
                     /translation="ALVGTQAVIPSTAVLGRVPTGGPKSFAPFTGPAVTAGGAMAGTA
                     GGTLVLFTQTISDANALGGRSDDVQMRIDLTGAPKLPAGSYTGTLNLLAITQ"
     gene            298..1044
                     /locus_tag="AC16JC_08230"
     CDS             298..1044
                     /locus_tag="AC16JC_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164720.1"
                     /translation="MRSCLPGNTALQRTVRRASLALGALASLAPPALTAQTVSPPIAE
                     YQERARSSFQLINSTIFPLSVVLEVHGFNVTEQGEVQEAPLDTTKVRVKLSAMSFRIP
                     PRQTYTVFYEASADSTPAWFNIVSAMTGARTENGLNVRILLPHVVYLNQKEPLRKEQV
                     VLRAYEVDTAAGKLRVQLENLGPNLGRVQQVTALAGKTTSAPSAGFPLMPHMIRWTEV
                     DWPERVAPERVSVRFARFTIDTVLAAPASP"
     gene            1049..>2803
                     /locus_tag="AC16JC_08235"
     CDS             1049..>2803
                     /locus_tag="AC16JC_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR5164721.1"
                     /translation="MTGRRRSALTSAAAILAAALGASGQLAAQTVMEVQGGGSSLVGG
                     YGATANFWRNGMDGWVGLGYLDGLRVGAFLRTAVGKDTVRLGNDALVMHFPTDLFSSG
                     YNLLVQGVSVGGGTTDRSYLAFAGASSSSLAAPSFQATRIEKPMGALFLQRRISPTVR
                     LTGNVLIARRQTIMPGIQWQATPDLTTALVAGTGAGSFYAAGAATLRRGPLGVQAMYA
                     WNPDRFRRADVPGPTQTEAERENIAVTYDVGSGFQVGVARQHFLQDSADGAAPIRATG
                     NSAFASGRVSDIRVTGGLYDSRSQGVSNLSSYFAVGRELTGWLDAELFLLQSRPSGRA
                     SSTTPIINLRWRVSPRVRLMQQLSVHDHRPTILFGANLITALGEFGADYQIVHQPFEP
                     FKPFRSTLNLTARLQLGRYSTNLGTSVRPDGSVDYSASGSTFLYMGAFGGVQPQQLGQ
                     NLGRYIVRGIVKDDQGQPVEGAAVDLSGEMAFTNSRGEFFVRVRRPQRYALAVPLDEF
                     LLPGRWEVVAAPSEAIAESEEQARPVEIILRRVRQAPAPAPPAMPADTSVWQTADTLH
                     RLSSDGAPTQRPGPVSRLS"
BASE COUNT          421 a         1012 c          956 g          414 t
ORIGIN      
        1 gccctggtcg gcacccaggc ggttattccc tcgacggcgg tcttgggacg ggttccgacc
       61 ggcggaccga agagcttcgc cccgttcacc ggcccggccg tgacagccgg cggggccatg
      121 gccggcaccg ccggcgggac actggtgctg ttcacgcaga cgatctccga cgccaacgct
      181 ctgggcggac ggagcgacga cgtgcagatg aggatcgacc tgaccggcgc gcccaagctc
      241 ccggccggga gctataccgg gaccctcaat ctgctggcga tcacgcaatg aggctctatg
      301 cgctcgtgcc tgcccggcaa cactgctctc cagcggacgg tccgccgcgc tagcctcgcg
      361 ctcggcgccc tcgcttctct tgcaccgcct gccctgaccg cgcagaccgt cagccccccg
      421 atcgccgaat atcaggaacg cgcccggtcg agcttccagc tcatcaactc gaccatcttt
      481 ccgctcagcg tcgtgctcga agtgcacggg ttcaacgtga cggagcaggg cgaggtacag
      541 gaggccccgc tcgataccac gaaggtccgg gtcaagctgt cggcgatgag cttccgcatc
      601 cccccgcgcc agacctacac ggtgttctac gaggcgagcg ccgacagcac cccggcctgg
      661 ttcaacatcg tgagcgccat gaccggcgcc aggaccgaga acgggctcaa cgtccgcatt
      721 ctcctcccgc acgtggtcta cctcaaccaa aaggagcccc tccggaagga gcaggtggtc
      781 ctgcgggcgt acgaggtcga cacggccgcc gggaagctgc gggtgcagct cgagaacctc
      841 ggccccaacc tcggccgcgt gcagcaggtg accgcgttgg cgggcaagac caccagcgca
      901 ccgagcgccg ggtttccgct gatgccgcac atgatccggt ggaccgaggt ggattggccc
      961 gagcgggtcg cacccgagcg ggtatcggtc cgctttgcgc gtttcaccat agacacggtg
     1021 ctggccgcac cggcgtcgcc ctagcaccat gaccggccgg aggcgctccg ccctcaccag
     1081 cgccgccgcg atcctcgcgg cggcgctcgg cgcgtccggc cagctggcgg cgcagacggt
     1141 gatggaggtc cagggtggcg ggtcgtcgct ggtaggcggc tacggcgcca ccgcgaactt
     1201 ctggcggaac ggcatggacg gctgggtcgg gctgggctac ctcgacgggc tccgggtcgg
     1261 cgccttcctg cggacggccg tcgggaagga caccgtgcgc ctcggcaatg acgccctggt
     1321 gatgcacttc ccaaccgacc tgttcagcag cggatacaat ctgctcgtcc agggcgtcag
     1381 cgtgggcggc ggcaccaccg accgatccta cctggcgttc gcgggcgcca gctcgagcag
     1441 tctggccgcg ccgtcgttcc aggccacccg catcgagaag ccgatgggcg cgctcttcct
     1501 gcagcgccgg atctcgccca cggtgcggct caccggcaac gtgctcatcg cccgccgcca
     1561 gaccatcatg cccggcattc agtggcaagc cacgccggat ctcaccacgg cgctggtggc
     1621 cggtaccggg gcaggcagtt tctacgcggc gggagcggcg acgctccggc gcggccccct
     1681 cggcgtccag gcgatgtacg cctggaatcc cgaccggttc cgccgggccg acgtgccggg
     1741 tccgacgcag acggaggcgg agcgcgagaa catcgccgtg acgtacgatg tgggctcggg
     1801 gttccaggtc ggcgttgccc ggcagcactt cctgcaggat tccgccgatg gagcggcgcc
     1861 catccgggcc accggcaact cggccttcgc gagcggccgg gtgagcgaca tccgagtcac
     1921 cgggggactg tacgattcgc gctcgcaggg cgtcagcaac ctcagctcct acttcgcggt
     1981 cgggcgcgag ctcacgggct ggctcgatgc cgagctgttc ctgctccaga gccggccgtc
     2041 gggccgggcg tcgagcacga cgccgatcat caacctgcgg tggagagtct caccccgggt
     2101 gcgcctgatg cagcagctct cggtccacga tcaccggccg accattctgt tcggcgccaa
     2161 cctgatcacc gcgctgggcg agttcggagc cgactaccag atcgtccacc agccgttcga
     2221 gccgttcaag ccgttccgca gcaccctcaa cctgacggcg cggctgcagc tgggtcgcta
     2281 ctcgaccaac ctcggcacgt ccgtgcgtcc cgacggctcg gtggactact ccgcgtcggg
     2341 cagcactttc ctctacatgg gcgcgttcgg cggggtgcag ccccagcagc tcggccagaa
     2401 cctcggccgg tatatcgtgc ggggaatcgt caaggacgac cagggacagc cggtcgaagg
     2461 cgcggcggtg gacctgagcg gcgagatggc attcaccaac tcgcggggcg agttcttcgt
     2521 gcgcgtccgc cggccgcagc gctacgcgct cgccgtgccg ctcgacgagt tcctgctgcc
     2581 cggccgctgg gaggtcgtag ccgcgccctc ggaggcgatt gccgaatcgg aagaacaggc
     2641 gcggccggtc gagatcatcc tgcggcgtgt ccgccaggcg ccggctcccg cgccgcccgc
     2701 gatgccggcg gatacctcgg tctggcagac ggccgacacc ttgcaccggc tgagcagcga
     2761 tggggcgccg acgcagcggc ccgggccggt gagccgcctc tcg
//
LOCUS       DBNGZW010000349         8644 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1795532, whole genome shotgun sequence.
ACCESSION   DBNGZW010000349 DBNGZW010000000
VERSION     DBNGZW010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8644)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8644)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8644
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1795532"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="AC16JC_08240"
     CDS             complement(<1..271)
                     /locus_tag="AC16JC_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013453235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR5164722.1"
                     /translation="MDPVVFWDNAIVYFLLTDRFLDGDSANDHALGRAHDGALLRNFE
                     GGDLAGVRRKIDEGYFDSLGVTAIWLTPFVEQIHGSVDEGTGKTYG"
     gene            complement(397..1935)
                     /locus_tag="AC16JC_08245"
     CDS             complement(397..1935)
                     /locus_tag="AC16JC_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5164723.1"
                     /translation="MQSSVVRPKPRLSFWEIWNMSFGFLGIQIGFALQNANVSRIFET
                     LGAKVENIPILWIAAPVTGLVVQPIVGYFSDRTWNRLGRRRPYFLGGAVLASLALIVM
                     PNSPVLWVAAGMLWIMDASINISMEPFRAFVGDNLPSSQRTTGFAMQSFLIGTSAVVA
                     SMLPWLLTTQFGVSNQAPERTIPDSVRLSFYFGAAVFFAAVLWTVIKSKEYSPAEMAS
                     FEENQAREVHARKTRSAEEFASNGRRQVFVGLILLVAGAVASAVLVALGEYQVIILSA
                     GAGVVGVLLIIGGLMQQRGRYDNGFVTILNDFQDMPRTMKQLAWVQFFAWFALFAMWI
                     YTTAAVTSHIYHTSDTTSALFNEGANWVGVGFSTYNGVAAVVAFAIPVLAARTSRKLA
                     HAICLVCGALGLLSIFILTDPRYLLGSMVGVGIAWASILSMPYAILTGSLPPSKMGYY
                     MGVFNFFIVIPQIVAAAILGRIVGGLFGGEAIYALLIGGASLVLAAILTLRVEDRDDV
                     KAAA"
     gene            complement(1950..3851)
                     /locus_tag="AC16JC_08250"
     CDS             complement(1950..3851)
                     /locus_tag="AC16JC_08250"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF014499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HIR5164724.1"
                     /translation="MDELRTLRSLARTMGVHTRYTDGLGRHVLVSPETLLRVCAALGA
                     PVAGAGDAADALRALDTGGTMDLVPPVLVAWDGVLPPLEIVADGPIHAELRLEDGGVV
                     PLETSSLPFGYHRLTVEADGRRETCTIIAAPVETWRRPDSPRSWGVGAHLAALRSARS
                     RSLGDLRDLESLCRWVNGRGGDLVTVLPLLPTFNSAPPEPSPYSPVSRLFWSELILDL
                     GDAHRPVAPPPPTLDVTRADAEVRAALREQPVPADPEADEELIRYAMFRGAQARLGRN
                     WRDWPAAARSGTLTPDQVDAAEERFHLVAQTEVRRQLRDLRQRLEHDGMRLGLDLAVG
                     VHPDGYDPWSRQGLFADGMAVGAPPDQGFPSGQDWGFSPVLPAASRREGHRYLARSIA
                     HQMELAGVLRVDHIMAWTRLYWIPHGLELHQGTYVSYPAEELFAILTLESNRHRCEVV
                     GENLGTVPRDIDEALPRHRIWGMYLAQFQAACGDDDLAPPDASDMALIGTHDTPTFAG
                     WLAGNDIDERVRYGLLAEASAPLAREERVSATQCLCDQLGCRLEDPQAFLGALLEWLG
                     RSESPLVVPWLEDLWLEEVAVNLPGTPSSQRPNWQRPMRRLLDEILTDVEIDGLIRRL
                     ERARKSPHS"
     gene            complement(3876..4088)
                     /locus_tag="AC16JC_08255"
     CDS             complement(3876..4088)
                     /locus_tag="AC16JC_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164725.1"
                     /translation="MKLDLRLPIGLMFTIFGVILTLYGFASDRAIYGRSFGINVNLWW
                     GLVLLAFGLVMLAFALRGGRKADRGS"
     gene            complement(4093..5793)
                     /locus_tag="AC16JC_08260"
     CDS             complement(4093..5793)
                     /locus_tag="AC16JC_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020721592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HIR5164726.1"
                     /translation="MQLGAVDYTLLIVYFAFVLGIGWVLRRHVRSSEDFFLSGRSIPA
                     WIAGLAFLSANLGAQEVIGMAASGAKYGMMTSHFYWVGAIPAMVFVGVFMMPFYYGSR
                     ARSVPEYLKLRFDEKTRGFNAITFAVMTVFSSGISMYALAKLLELVLGWSFHMSVILS
                     AVIVLAYIFLGGLTSAIYNEVLQFFLIVLGFLPLVLLGLKDVGGWAGLTAKLQTVATN
                     AGFSAGAWSDSWRHMNSASANPMGVEWFGMVMGLGFVLSFGYWCTDFLVVQRAMAANS
                     MTAARRTPLLAAFPKMLFPFLVILPGMIVIALQAGGGGLLPIGNDGTPNYNLAVPVML
                     AHYLPSGLLGLGLTALMASFMSGMAGNVTAFNTVWTYDIYQSYIRPGRPDAHYLRMGH
                     AATVFGILASVAAAYATTKFNNIMDMLQLVFAFVNAPLFATFLLGMFWRRTTGHAAFF
                     GLLTGTLVAALHHGLVLPQGAAAGLKGGFLGAVLHTYPSEMAQNFWTAIFAWTSCFVA
                     TIVISLLTRPNKSDEDLKGLVYALTPRQRDEHLPWFKRPATLGVVVLALTLVLNIIFW
                     "
     gene            complement(5881..6324)
                     /locus_tag="AC16JC_08265"
     CDS             complement(5881..6324)
                     /locus_tag="AC16JC_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164727.1"
                     /translation="MRVVAIQVSEKDLAQFEMAVAIACVRKSDKPLEFQRPTNIDRDA
                     RTLPPCFTQDGCVAISFDRSWNPFLCNTGDDASPSRWFIPKSSWLIAAVARALQTPMQ
                     RAISFSAGGRILLDAAGGRRQPEGHAEIAVVQWSLPRESPLLPTR"
     gene            complement(6452..8551)
                     /locus_tag="AC16JC_08270"
     CDS             complement(6452..8551)
                     /locus_tag="AC16JC_08270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR5164728.1"
                     /translation="MTTRREFVTTVSATGAGLLLGFRLPQRLSATPRGTAGDGELNAW
                     IRIGSDDQVTLIVNESEMGQGVLTSLPMILGEELDVDWTKVRSEHALADPRYGNQGTG
                     GSSSVRSDYATLRKAGAAARQMLIAAAAATWGVPAAECATEQGAVTHAKSGRRASYGR
                     LAVRAAALTPPADPPLKDPKSFRIIGKATKRLDTPAKVTGRAVFGIDVQVPGMLVAQV
                     AHCPTFGGKLDRVTPAAALLVPGVRHVVEIPTGVAVVADNFWAAQKGRDVLRVQWEPG
                     PAGELSSVTIAARLRELAPGGATAIRRGDPDAALGAAARRVDAEYEVPYLAHATMEPM
                     NCTADVRAGACEIWAPTQFQTSSRQLAAQITGLPLEHVTLHTTFMGGGFGRRAQSDFL
                     ADAVHTSKAVGRPVKVIYTREDDMHAGWYRPVAHNAFSAALDADGWPTAWVHRIASPS
                     ILANMGPLQDGIDRTSVEGAQNLPYAIPNLLVTYAKADFPIPVWFWRSVGSSINGYVT
                     ECFLDELALAGGKDPVELRRRLLSDQPRHRRVLDLAAQKAGWGTPLPAGRARGVAVHE
                     SFGSIVAEVAEVSLGEGGEPRVHRVVCAVDCGQYVNPDTVVAQMESGIVYGLSAALYG
                     EITIDKGRAVQDNFDSYQVLRIDQMPPVETHIVAEGDPMGGIGEPGTPPIAPAVCNAL
                     LVLTGKPVRRLPIVKET"
     gene            complement(8548..>8644)
                     /locus_tag="AC16JC_08275"
     CDS             complement(8548..>8644)
                     /locus_tag="AC16JC_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020596702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5164729.1"
                     /translation="AAMMGNICRCGTYNRIRRAIHRAAGESAGAP"
BASE COUNT         1434 a         2940 c         3019 g         1251 t
ORIGIN      
        1 agccgtaggt cttcccggtc ccttcgtcca cgctgccgtg gatctgctcc acgaacggcg
       61 tcagccagat ggccgtcacg ccgagactgt cgaagtagcc ttcgtcgatc ttccggcgga
      121 ctccggccag gtctccgccc tcgaagttac gcagcagcgc gccgtcgtgc gcgcggccca
      181 gcgcgtgatc gttggcggaa tcgccgtcga gaaagcgatc ggtgaggagg aagtagacga
      241 tggcgttgtc ccagaagacg accgggtcca cgtgacgcac cgatggcgcg gttactggcg
      301 tgggctcatg ggtttgggct tcgggggttg gatgagcaca ggcgacaccg gcgcccgcgc
      361 cgacgacccc gagcgtcgcg gcgcgatgga tcctgttcat gcggcggcct tcacgtcgtc
      421 ccggtcctcg acgcgcagcg tgaggatcgc ggcgagcacg agcgacgcgc cgccgatcaa
      481 caacgcgtag atggcttcgc cgccgaacaa tccgccgacg atgcgaccca ggatggcggc
      541 cgcgacgatc tgggggatca cgatgaagaa attgaacacc cccatgtagt agcccatctt
      601 cgagggcggc agggagccgg tcaggatcgc gtagggcatg gagaggatgc tcgcccaggc
      661 aatcccgact cccaccatgg agccgagcag gtaccggggg tccgtgagga tgaagatgga
      721 aaggagcccc agcgcgccgc acaccaggca gatcgcgtgg gcgagcttcc ggctggtccg
      781 cgccgccagc accggaatgg cgaaggccac caccgcggcg acgccgttgt acgtgctgaa
      841 cccgaccccg acccagttgg cgccctcgtt gaacagcgcg gaggtcgtgt cgctggtgtg
      901 gtagatgtgg ctggtcaccg ccgcggtagt gtagatccac atggcgaaca gcgcgaacca
      961 cgcaaagaac tggacccagg ccagctgctt catggtccgg ggcatgtcct ggaaatcgtt
     1021 caggatggtg acgaacccgt tgtcgtagcg gccgcgctgc tgcatgaggc cgccgatgat
     1081 gagcagaacc ccgacgaccc ccgcgcccgc cgagaggatg atgacctggt attcccccag
     1141 ggcgacgagc accgcgctgg cgacggcgcc cgcgacgagc aggatcaggc ccacgaagac
     1201 ctgccgccgc ccgttgctcg cgaactcctc cgccgagcga gtcttcctcg cgtggacctc
     1261 gcgcgcctgg ttctcctcga acgaggccat ctctgcgggc gaatactcct tcgacttgat
     1321 caccgtccag aggacggcgg cgaagaagac cgcggcgccg aaatagaagg acaggcgcac
     1381 cgagtcgggg atcgtgcgct cgggagcctg attgctgact ccgaactggg tggtgaggag
     1441 ccacgggagc atggaggcta cgacggcgct cgtgccgatg agaaagctct gcatcgcgaa
     1501 ccccgtggtg cgctgcgacg aggggaggtt gtcccccacg aaggcgcgga acggctccat
     1561 ggagatgttg attgaggcgt ccatgatcca cagcatgccg gccgcgaccc acagcaccgg
     1621 ggaattcggc atgacgatca gggccagcga agcgaggacg gctccgccga ggaagtaggg
     1681 ccggcgccgg cccaggcggt tccaggtgcg gtcgctgaag tagccgacga tgggctggac
     1741 cacgagcccc gtcaccggcg ccgcgatcca caggatgggg atgttctcca ccttcgcgcc
     1801 cagcgtctcg aagatccggc tcacgttcgc gttctgcagc gcgaagccga tctggatccc
     1861 gagaaacccg aagctcatgt tccagatttc ccagaacgaa aggcgcggct tgggccggac
     1921 gaccgacgat tgcatggtgg atctggacct caggagtggg gtgacttccg ggcgcgctcc
     1981 aggcgacgga tcaatccatc aatctcgacg tccgtgagga tctcgtcgag cagccgccgc
     2041 atcgggcgct gccagttcgg tcgctgcgac gatggcgtgc cggggagatt gacggcgact
     2101 tcctccagcc agagatcctc gagccagggg acgaccagcg ggctctccga gcggccgagc
     2161 cactcgagca gtgcgcccag gaaggcctgg ggatcttcca gccggcagcc caattggtcg
     2221 cagagacact gcgtggcgct cacccgctcc tcccgggcga gcggggcgct cgcctccgcg
     2281 agcaggccgt agcggacgcg ctcgtcgatg tcgttgccgg cgagccaccc cgcgaacgtg
     2341 ggcgtgtcgt gcgtgccgat cagcgccatg tcgctcgcgt ccggcggggc gaggtcgtcg
     2401 tcgccgcacg cggcctggaa ttgcgccaga tacatccccc agatccggtg gcgcggcagc
     2461 gcttcgtcga tatcgcgggg gacggtgccc agattctcgc ccaccacctc gcagcggtga
     2521 cggttcgact ccagggtgag aatcgcgaac agctcctcgg ccggatagct gacgtaggtc
     2581 ccctggtgca gctcgagccc gtgggggatc cagtagaggc gggtccaggc catgatgtgg
     2641 tccacgcgga gcactccggc gagctccatc tggtgtgcaa tggaccgggc caggtaacgg
     2701 tgcccctcgc gccgggacgc cgcggggagc accgggctga acccccagtc ctgaccgctc
     2761 gggaacccct ggtcgggagg cgcgcccacc gccatcccgt ccgcgaagag gccctgccgc
     2821 gaccacggat cgtagccgtc ggggtggacg ccgaccgcga ggtccagccc cagacgcatc
     2881 ccgtcgtgct cgagccgttg ccgcaggtcg cgcagttgcc ggcgcacctc cgtttgcgcg
     2941 accaggtgga agcgctcttc ggcggcatcc acctgatcgg gcgtcagcgt cccggaccgg
     3001 gcggccgccg gccaatcgcg ccagttccgc cccagccgcg cctgagcgcc gcgaaacatc
     3061 gcgtagcgga tcagctcttc gtccgcctcc gggtcggccg ggaccggctg ctccctgagc
     3121 gccgcgcgga cctccgcgtc cgcccgggtc acgtccagcg tggggggggg aggggcaacg
     3181 ggacgatggg catcgcccag gtcgaggatc agctcggacc agaagaggcg gctcaccgga
     3241 gagtatggac tgggctccgg cggcgcggag ttgaacgtgg gcagcagcgg cagaaccgtg
     3301 acgagatctc cgccgcgccc gttcacccag cgacacagcg actcgaggtc gcgcaggtcg
     3361 cccagtgagc ggctgcgtgc cgagcggagg gcggcgagat gcgcgccgac tccccaactc
     3421 cgcggggaat ccggccggcg ccatgtctcg accggcgcgg cgatgatcgt gcacgtctcg
     3481 cgccggccgt cggcctcgac cgtcagccgg tggtagccga acgggagcga ggacgtctcg
     3541 agcgggacga cgccgccgtc ctcgagccgc agctccgcgt ggatggggcc gtcggcgacg
     3601 atctcgagag gaggcaggac cccgtcccag gcgacgagca cgggcgggac gagatccatc
     3661 gttccgccgg tatcgagtgc ccgcagcgcg tccgcggcat ccccggcgcc ggccaccggg
     3721 gcgcccagcg ccgcgcagac gcgcagcagg gtctcgggcg acaccaggac gtgcctgccg
     3781 agaccgtcgg tgtaccgggt atggaccccc atcgtcctgg cgagggagcg cagtgtcctc
     3841 agctcatcca tgcgcgaggg gggcgcggcg ccagctcacg agccgcggtc ggccttcctc
     3901 ccgccccgga gcgcgaaggc gagcatcacg aggccgaacg cgagcagcac cagtccccac
     3961 cacaggttca cgttgatgcc gaacgaccgg ccgtagatcg cgcggtccga cgcgaacccg
     4021 tacagcgtca ggatcacgcc gaagatcgtg aacatcagcc cgatcggcag ccgcaggtcc
     4081 agtttcatcg catcaccaga agatgatgtt gagcacgagg gtgagcgcca gcacgacgac
     4141 gcccagcgtg gcggggcgct tgaaccacgg caggtgctcg tcccgctgcc gcggggtgag
     4201 ggcgtacacc agccccttca ggtcctcgtc cgacttgttg ggccgcgtga gcagcgagat
     4261 cacgatcgtc gccacgaagc aggacgtcca cgcgaagatg gcggtccaga agttctgcgc
     4321 catctcgctc ggataggtgt gcagcaccgc gccgaggaat ccgcccttga ggccggctgc
     4381 cgcgccctgc ggcagcacga gtccgtggtg cagggcggcg acgagggtgc ccgtgagcag
     4441 tccgaagaac gcggcgtgac ccgtggtgcg gcgccagaac atgcccagca ggaacgtcgc
     4501 gaacagcggc gcattcacga acgcgaacac cagctgcagc atgtccatga tgttgttgaa
     4561 cttcgtggtc gcgtatgccg cggccacgct cgccaggatg ccgaagacgg tggccgcgtg
     4621 ccccatccgc aggtagtgcg catccggccg gcccggccgg atgtaggact ggtagatgtc
     4681 gtaggtccac acggtgttga acgccgtgac gttgcccgcc atgcccgaca tgaacgaggc
     4741 catgagcgcc gtgaggccga ggcccagcag gcccgacggc aggtaatgcg cgagcatcac
     4801 cggcaccgcg aggttgtagt tgggtgtgcc gtcgttgccg atcggcagca ggccgccgcc
     4861 gccggcctgc agcgcgatga cgatcatccc ggggaggatc accaggaacg ggaacagcat
     4921 cttggggaat gccgcgagca gcggcgtgcg ccgcgcggcg gtcatcgagt tggcggccat
     4981 ggcccgctgc acgacgagga agtcggtgca ccagtagccg aacgacagca cgaaccccag
     5041 ccccatcacc atcccgaacc attccacgcc catgggattg gcgctggccg agttcatgtg
     5101 acgccacgag tcggaccagg ccccggcgga gaaccccgcg ttggtcgcga cggtctggag
     5161 cttcgcggtc aggccggccc agccgcccac gtccttgagg ccgagcagga ccagcggcag
     5221 gaagcccagc acgatgagaa agaactgcag cacctcgttg tagatcgccg acgtcagtcc
     5281 gccgaggaag atgtaggcga ggacgatgac ggcggacaga atgacgctca tgtggaagct
     5341 ccagccgagc acgagctcga ggagcttcgc cagcgcgtac atcgagatcc cggaggagaa
     5401 cacggtcatc acggcgaacg tgatggcgtt gaagccgcgg gtcttctcgt cgaagcgcag
     5461 cttcaggtat tcggggacgg agcgcgcgcg tgagccgtag tagaacggca tcatgaagac
     5521 gccgacgaac accatggcgg ggatggcgcc cacccagtag aagtggctcg tcatcatccc
     5581 gtacttggcg cccgaggcgg ccatgccgat cacttcctgc gcgccgaggt tcgccgagag
     5641 aaaggcgagg ccggcgatcc acgcgggtat cgagcgtccc gagaggaaga agtcttcgct
     5701 gctcctgacg tggcgccgca gtacccagcc gatgccgagc acgaaggcga agtagacgat
     5761 caggagggta tagtcgacgg ctccgagctg gatgtgctgc acgggcggtg ctcgcggaaa
     5821 acggtgccta ccatgtacac cgcttcgggc cgggggcgca agccgggccc gaggccgccg
     5881 ctagcgcgtc ggcagcaggg ggctttcccg tggcaggctc cactgcacca ccgcgatctc
     5941 ggcatggcct tccggctgac gtcgcccgcc cgcggcgtcc agcaggatcc gtccacccgc
     6001 ggagaagctg atggcccgtt gcatgggggt ctgcagggca cgcgcgaccg ccgcgatgag
     6061 ccagctcgat ttcggaatga accagcgcga tgggctcgcg tcgtcaccgg tgttgcagag
     6121 gaagggattc cagctccggt cgaacgagat cgccacgcag ccgtcctggg tgaagcaagg
     6181 cggcagggtg cgggcatcgc ggtcgatgtt cgtgggacgc tgaaactcca gtggcttgtc
     6241 ggacttccgc acgcaggcga tggcgacggc catctcgaac tgggcgaggt ccttctccga
     6301 tacttgaatg gcaaccacac gcacctgccg atcctgtcgt gactgcaagg aagggccggg
     6361 gccgggcggg acccggttcc ggctccggcg ctcgccggga tactcttgaa tgctacactg
     6421 gaggcgccgc cttccgtgta acgaaattct cctaggtctc cttcacgatc ggcagccgcc
     6481 gcaccggttt tccggtgagc acgagcagcg cattgcacac cgccggtgcg atggggggcg
     6541 ttcccggctc gccgatcccg cccatcgggt cgccctcggc gacgatgtgg gtctccaccg
     6601 gaggcatctg gtcgatccgc agcacctggt aggagtcgaa gttgtcctgg acggcccgcc
     6661 ctttgtcgat cgtgatctcg ccgtacaacg ccgccgagag cccgtagacg atcccgctct
     6721 ccatctgggc cacgaccgtg tcggggttca cgtactggcc gcaatcgacg gcgcacacga
     6781 cacggtgcac gcgcggctcg ccgccttcgc ccaacgacac ctcggccacc tcggccacga
     6841 tgctcccgaa cgactcgtgc acggccacgc cgcgcgcccg gcccgcgggg agcggcgtgc
     6901 cccacccggc cttctgcgcc gcgaggtcca acacgcggcg gtggcgcggc tggtcgctca
     6961 acaggcggcg gcgcagctcc accggatcct tcccgcccgc cagcgccagc tcgtccagga
     7021 aacattcggt cacgtacccg ttgatcgagc tgcccaccga gcgccagaac cataccggaa
     7081 tcgggaaatc cgccttggcg tacgtgacca gcagattggg aatggcatag ggcaggttct
     7141 gcgcgccctc gaccgaggtg cggtcgatgc cgtcctgcag cggccccatg ttcgccagga
     7201 tcgacgggct cgcgatacga tgcacccacg ccgtgggcca gccgtcggcg tcgagcgcgg
     7261 cgctgaacgc gttgtgcgcc accgggcgat accacccggc gtgcatgtcg tcctcgcgcg
     7321 tgtagatgac tttgaccggc cggcccaccg ccttcgaggt gtgaacggcg tcggccagga
     7381 aatcgctctg ggcccggcgg ccgaagccgc cgcccatgaa cgtcgtgtgc agcgtgacgt
     7441 gctccagcgg cagcccggtg atctgggcgg cgagctggcg cgacgaggtc tggaactgcg
     7501 tgggggccca gatctcgcac gcgccggcgc ggacgtcggc ggtgcagttc atcggctcca
     7561 tggtcgcgtg cgcgaggtag ggaacctcgt actccgcgtc cacgcggcgt gccgccgcac
     7621 cgagcgccgc gtcgggatcg ccgcggcgaa tcgccgtggc cccccccggc gcgagctcgc
     7681 gcaaccgcgc ggcgatcgtc acgctcgaaa gctcaccggc cgggcccggc tcccactgga
     7741 cccggagcac gtcacgccct ttctgggccg cccagaagtt gtcggcgacg accgccacgc
     7801 cggtcgggat ctcgacgacg tgccgtacgc ccggaaccag cagcgcggcc gccggggtga
     7861 cccggtcgag cttgccgccg aaggtggggc aatgcgccac ctgggccacg agcatgcccg
     7921 ggacctggac gtcgatgccg aagaccgccc gtcccgtcac cttggcgggt gtgtcgagcc
     7981 gcttggtcgc cttgccgatg atgcggaagc tcttcggatc cttgagcggc gggtccgcgg
     8041 gcggggtcag tgccgccgcg cgtacggcga ggcgcccgta actcgcgcgc cgcccgctct
     8101 tcgcgtgcgt gaccgcgccc tgctcggtgg cgcactcggc cgccgggacg ccccaggtgg
     8161 ccgccgccgc ggcgatcagc atctgccgcg ccgcggctcc ggccttgcgc agcgtcgcgt
     8221 agtcgctgcg cacgctcgag ctgccgccgg ttccctggtt gccgtaccga ggatcggcga
     8281 gcgcgtgctc ggagcgcacc ttggtccaat ccacgtccag ctcctcaccc aggatcatgg
     8341 gcagcgaggt gaggacgccc tggcccatct ccgattcgtt cacgatcagc gtgacctggt
     8401 cgtcgctgcc gatgcgaatc caggcgttga gctcgccgtc tccggccgtt ccccgcggcg
     8461 tcgccgagag ccgctgcggc agacggaacc cgagcaacag gccggcgccg gttgccgaga
     8521 cggtcgtgac gaattcgcgg cgggtggtca tggcgcccct gcgctttctc cggccgcgcg
     8581 gtgaatcgcg cgccggatgc ggttgtacgt accacagcgg cagatgttgc ccatcatcgc
     8641 tgcg
//
LOCUS       DBNGZW010000350         1719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1124223, whole genome shotgun sequence.
ACCESSION   DBNGZW010000350 DBNGZW010000000
VERSION     DBNGZW010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1124223"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1303
                     /locus_tag="AC16JC_08280"
     CDS             <1..1303
                     /locus_tag="AC16JC_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164730.1"
                     /translation="RALSALVALALPTARHASAQGSWHMSLGPEDSVDIGMLRNAPNG
                     HISYQQGKGGLTIWVDGRIGDATQGTFILHPHSWSIPELERAAPVRVFEAVHDSTLPC
                     TSDSGWYRNYAAINAIIPGPQPGELLAFVDGEFHPRNTGTPLRASIGVATSTDGGMTW
                     GQRAVIVQGNDMVAYHFDCRALDATMKTKVDDEGSAGPTAVVRTEGGTQYVYLYYFDR
                     VRLSSGGQPTSYIYVARAPYTHAGAPGSWQFWTDGRWSPQGHEVMASPVITTPPHGGA
                     AAHPLVTFNTALGRWLMIFHTRLDLYATTSEDGITWDTPQVLGASIRGARAPAFPTLV
                     TPGSGDQQTTGATGWLFYSREVNLSATRKGYLGFRRSFIISTGPRGSATSRHRPSRTA
                     SPRTGPDAHEARVQSPERRGRVDRRRADEDRAEPHGEHPSR"
BASE COUNT          264 a          617 c          574 g          264 t
ORIGIN      
        1 ccgcgccctc tcggctctgg tggcgctggc gttgcccaca gcgaggcacg cgtcggccca
       61 gggctcgtgg cacatgagcc tcggcccgga agactcggtc gatatcggca tgctgcgaaa
      121 cgcgccgaac ggccacatct cctaccagca aggcaagggc ggcctgacaa tctgggtgga
      181 cggccggatc ggcgacgcga cccaggggac gttcatcctg cacccgcata gctggagcat
      241 cccggagctc gagcgcgccg cgcccgtgcg ggtcttcgag gcggtccacg attccacgct
      301 cccctgcacg tccgactccg ggtggtaccg caactacgcc gcgatcaacg cgatcattcc
      361 cggcccgcag ccgggcgagt tgctggcgtt cgtggatggg gaattccacc ccaggaacac
      421 ggggacgccg ctgcgcgcgt cgatcggcgt tgcgacgtcc accgatggag gcatgacctg
      481 ggggcagcgg gcggtgatcg tgcagggcaa cgacatggtc gcgtaccact tcgattgccg
      541 tgccctcgat gccacgatga agacgaaggt cgacgacgag ggttccgccg gcccaacggc
      601 tgtcgtgcgc acggagggag ggacgcagta tgtctacctc tattatttcg accgcgtgcg
      661 gttgtcgtcg ggtggacagc cgacgtccta catctatgtc gccagggcgc cgtatacaca
      721 cgctggcgca ccggggagtt ggcagttctg gactgacggc cgctggagcc ctcagggaca
      781 tgaggtcatg gcgagtccgg tgatcacgac cccgccgcat ggcggcgcgg ccgcgcatcc
      841 gctggtaacc ttcaacaccg ccctcgggcg gtggttgatg atcttccaca cccgcctcga
      901 tctctacgcc acgacgtccg aggacggcat tacctgggac acgccgcagg tcctcggcgc
      961 gtcgattcgc ggggcccgcg cgccggcgtt tcccactctc gtgaccccgg ggtctggcga
     1021 ccagcagacc accggcgcca ccggctggct cttctattcg cgggaagtga acctgtcggc
     1081 cacgaggaag ggctatctcg gcttccgtcg ttcattcatc atcagcaccg gtccgagggg
     1141 ttcagcaact tcccgccacc gtccttcccg gaccgcgtcc ccccgcacgg gccccgatgc
     1201 ccacgaagcc cgtgttcagt ctccggagcg gcgtggtcgg gttgaccgga gacgtgccga
     1261 cgaagatcgt gccgagcccc acggagaaca cccatcgcgg tgagccggtc gtgaggccgt
     1321 gcgtcgcgtc cgctgtgagg gcgaggggac cggcgattcg gtagctcgtc cccgcgccga
     1381 tcactcgact cagcgcctgc gtcgagtcgg ccgctccgac gtccgcgccg agcgccagat
     1441 tggccgtcca acggtcggtg acgtcgaagc cgctctccag ccgaagcgcc gtggcgtgcg
     1501 gagcgctcag cgccgattgg gcgcccaggc cgcccagact gcggctcgcg ctcgccgaga
     1561 gtcgccaccg ctcgccgggc gctacgccga tgcccccgtc caggccgaag cccatctccc
     1621 cgctccccag cccgcagctc gcattgccga ccggcagact cgccaccagc gcggccgcga
     1681 tcacgggtga ccacgggccc gggaaggagt gatccgccg
//
LOCUS       DBNGZW010000351         4690 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_511823, whole genome shotgun sequence.
ACCESSION   DBNGZW010000351 DBNGZW010000000
VERSION     DBNGZW010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4690)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4690)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4690
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_511823"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="AC16JC_08285"
     CDS             <1..321
                     /locus_tag="AC16JC_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018127559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164731.1"
                     /translation="RRAIAVEGSLFDVHLTGAREDAFRHPMQLWGRLAALQSDVSENS
                     ADFAPTAQQLAVNELFRQRLAEATTRFADLMGKDLPAFAATLRQTPLRDIIATGISAG
                     APER"
     gene            345..1220
                     /locus_tag="AC16JC_08290"
     CDS             345..1220
                     /locus_tag="AC16JC_08290"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002623799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HIR5164732.1"
                     /translation="MRLHLVDGTYELFRAHFGAPPTLAPDGRAVGATVGLLRSLLSLL
                     REDGVTHVACAFDTVIESFRNELFPGYKTGEGVPEELMAQFPLAERAARVLGVVVWPM
                     VEFEADDALATAAARGHASPEVEQVVICSPDKDLAQCVRDTRVVCLDRRRRLLYDRAG
                     VLEKFGVAPASIPDWLALVGDTADGFPGVPRWGPRSAATLLAHYEHLEAIPDDPVHWA
                     VSVRGADALARSLREHREDAALYRKLATLRTDVPLAEDPESLRWRGALRAELEDLCRE
                     LGSEDIVERVHRWRD"
     gene            1312..2067
                     /locus_tag="AC16JC_08295"
     CDS             1312..2067
                     /locus_tag="AC16JC_08295"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5164733.1"
                     /translation="MQYRSPAGVVYRAQRDTGAIAHAESALALDPRNVDLIIQLGVAQ
                     SGARQYREAIKTFTRGLALAPDNALLYRWRGHRYLSVREFGHAMEDLTHGLSLDSTLY
                     GILYHLGIVRYARGDFERAADAFARAQPRAPDAGELAGATDWLWMSLARAGKPVEANA
                     MLARHPDSLPVDNAYARRLRLYRGEIGPEAVITPADTEDIAVATLSYGLGNWYLVRND
                     TTQARAWFERSVKSGGWPAFGFIVSEIELRRVH"
     gene            2067..2609
                     /locus_tag="AC16JC_08300"
     CDS             2067..2609
                     /locus_tag="AC16JC_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008270062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5164734.1"
                     /translation="MEFDLERSIDVLRRTPATLRALLDGMDESLIRGTEGPDTFSAFD
                     NVGHLIDGEETDWMTRARIILAQGPSIRFEPYDRFRHRTRNAGRTLASLLNEFERLRA
                     ANLETLRSWQLTDTQLDLPGQHPRLGRVTLRQLLAAWVVHDLGHLAQVSRVLAKQYTD
                     AVGPWVQFLPVLTDHPVPRS"
     gene            2622..3494
                     /locus_tag="AC16JC_08305"
     CDS             2622..3494
                     /locus_tag="AC16JC_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164735.1"
                     /translation="MKHIPVLLTISATALLPLRANAQQDTAAVRVLMQRGAQLDRDGA
                     TVQARVTFQALIDSAANPAARAAAQRAMAMSYAFDGDCANTMKYEDLVIAYWQTREQA
                     EPQNAFYQEGEMANEGARVCLEAGDVDGAERWYRRGYELGVKEPAPPTHPRSLWDYRL
                     AHALGRIAARRGNTEEAQRQIAAARRALDGDTAMAAQQERFFPYLVGYVALYTNDLAT
                     AEAQLTKGLALRGNERDPFLHCLLAMTLEKLGRQDDARAAYQRAYDLATAHNPPAAFV
                     RPFARKKLSLPASR"
     gene            3505..4119
                     /locus_tag="AC16JC_08310"
     CDS             3505..4119
                     /locus_tag="AC16JC_08310"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004089 - carbonate dehydratase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HIR5164736.1"
                     /translation="MPAVEALGRLREGNLRFASGASALQDALSRGRRAELAERQEPFA
                     IILGCSDSRVPAEIVFDQGLGDLFVIRVAGNIVAPSQVGSIEFAAARFGTQLVVVLGH
                     SRCGAILATLEELRTPTERQSRNLRAIVDRVRPSVEGLLATELRDDPEALVHQAVRAN
                     IRASVDHLRHGSELLEQLALQGRLLVVGAEYSLETGLVEFFDAV"
     gene            complement(4171..4524)
                     /locus_tag="AC16JC_08315"
     CDS             complement(4171..4524)
                     /locus_tag="AC16JC_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HIR5164737.1"
                     /translation="MSILGNLMSKIFGRSEAAPSPTATGAPAAVATVVDIEAVLTQMA
                     AKNPQKLNWRTSIVDLMKLVGMESSLTERKELAKELGYTGNMQDTAAMNIWLHKQVLR
                     KMVENGGKVPANLLD"
BASE COUNT          698 a         1637 c         1606 g          749 t
ORIGIN      
        1 cgccgcgcga tcgccgtcga aggcagcctg ttcgacgtcc acctgaccgg ggcccgggag
       61 gatgcgttcc gccacccgat gcagctctgg ggccggctcg cggcgctgca atcggacgtc
      121 tccgagaaca gcgccgattt cgctccgacg gctcagcagc tcgcggtgaa cgagctgttc
      181 cgccagcgcc tggcggaggc cacgacgcgg ttcgccgatc tgatgggcaa ggacctgccg
      241 gcgttcgccg cgacgctgcg tcagaccccg ctccgcgaca tcatcgccac ggggatttcg
      301 gcgggagccc ccgagcggta ggcggagagc acgagcgatc gtccatgcgt ctgcatctgg
      361 tcgacggcac ctacgagctg tttcgggccc acttcggcgc tcctccgacg ctcgccccgg
      421 acggcagggc ggtcggggcc accgtggggt tgctgcgctc gctgctgtcc ctgctgcgtg
      481 aggacggggt gacgcacgtc gcctgcgcgt tcgacaccgt catcgagtcg ttccgcaacg
      541 agttgttccc cggctacaag accggcgagg gcgtgcccga ggagctgatg gcccagttcc
      601 cactggccga acgcgccgca cgggtactgg gtgtggtcgt atggccgatg gtcgagttcg
      661 aggcggacga tgcgctggcg accgcggcgg cgcggggcca cgcgtctccg gaggtcgagc
      721 aggtcgtgat ctgctcgccg gacaaggatc tggcgcaatg cgtgcgcgac acccgcgtcg
      781 tgtgtctcga tcgccgccgc cgcctgctgt acgaccgagc gggcgtgctc gagaagttcg
      841 gggtcgcgcc ggcttcgatt cccgactggc tcgcactcgt gggtgacacg gcagacgggt
      901 tccccggcgt tccccgctgg ggccctcgct ccgccgcgac cctgttggcg cactacgaac
      961 acctggaagc gattcccgac gaccccgtgc actgggcggt gagtgtccgc ggcgcggacg
     1021 ccctcgccag gagcctgcgg gagcatcggg aggacgccgc gctctaccgg aagctcgcaa
     1081 cgctgcggac cgatgtgccg ctcgccgaag acccggagtc tttgcgctgg cgcggcgccc
     1141 tgcgcgccga gctggaggat ctctgccgtg agctcggcag cgaggatatc gttgagcgcg
     1201 tgcatcgctg gcgagactga ccactacggg gggcggccga tgcgaaaccg ctggatatcg
     1261 accctatggc tcgcggccgc tgtcatcccc ggcccagcct ccgggcagtc ggtgcaatac
     1321 cgctcacccg ccggcgtcgt atatcgggcg cagcgcgaca ccggtgcgat cgcccacgcc
     1381 gagagcgcgc tcgccctcga cccgcggaac gtggatctga tcatccagct gggcgtcgcc
     1441 caatcgggcg cccggcagta ccgcgaggcg atcaagacct tcacccgtgg gctggcgctc
     1501 gcgcccgaca acgccctgct gtaccgctgg cgtggccatc gctatctctc ggtccgcgag
     1561 ttcggccacg cgatggagga tctgacgcac ggactgtcgc tcgacagcac cctgtacggc
     1621 atcctgtatc acctggggat cgtgcgctat gcgcggggcg atttcgagag agccgccgac
     1681 gccttcgcac gcgcccagcc gcgggcgccg gacgccggcg agctggccgg ggccaccgac
     1741 tggctctgga tgtcgctcgc ccgcgccgga aagccggtgg aggcgaacgc gatgctcgcc
     1801 cgccaccccg actcgcttcc ggtggacaac gcctacgccc ggcggctgcg cctgtaccgc
     1861 ggcgaaatcg gcccggaggc ggtgatcacc cccgccgaca ccgaggacat cgcggtcgcc
     1921 accctgagct acggcctggg caactggtac ctggtgcgca acgacacgac gcaggcgcgc
     1981 gcttggttcg agcgctccgt caagtcgggc ggctggccgg cgttcggatt catcgtctcg
     2041 gagatcgaat tgcggagggt gcactgatgg agttcgacct cgagcgctcc atcgacgtgt
     2101 tgcgccgtac cccggcgacg ctgcgcgcgc tgctcgacgg aatggacgag tcgctgatcc
     2161 ggggtaccga aggtcccgac accttcagcg ccttcgacaa cgtgggccac ctgatcgacg
     2221 gcgaggaaac cgactggatg acccgcgcac ggatcatcct ggcgcaaggg ccgagcatcc
     2281 ggttcgagcc gtacgatcgc ttccgccatc gcacgcgcaa cgccggtcgt accctggcgt
     2341 ccctgctgaa cgaattcgag cgcctgcggg ccgccaatct ggagacactc cggtcgtggc
     2401 agctgacgga cacccagctc gacctgcccg gacagcaccc gcgcctcggt cgcgtgacgc
     2461 tgcggcagct gctcgccgcc tgggtggtgc acgacctcgg ccacctggcg caggtctcgc
     2521 gcgtactggc gaagcagtac actgacgcgg tcggcccctg ggtgcagttt cttcccgtgc
     2581 tcaccgacca cccggtgccc cgatcctgag aggcaatcac catgaagcat ataccggtcc
     2641 tgctcacgat ctctgcgact gccctccttc ccctgcgggc gaacgcccag caggataccg
     2701 ccgcggtccg cgtcctgatg cagcgcggcg cgcagctgga tcgtgacggc gccacggtac
     2761 aggcgcgcgt gacgttccag gccctgatcg attcggccgc caatccggca gcccgggccg
     2821 cggcgcaacg cgccatggcg atgtcctacg ccttcgacgg cgactgcgcc aatacgatga
     2881 agtacgagga cctggtcatc gcctactggc agacgcgcga gcaggccgag ccgcagaacg
     2941 ccttctatca ggaaggtgag atggcgaacg aaggggcccg cgtgtgcctc gaggcgggcg
     3001 acgtggatgg cgccgaacgt tggtatcgcc gcggatacga gttgggtgtg aaagagccgg
     3061 ccccgccgac gcacccgcgc agcctgtggg actaccgtct cgcccatgcg ctcggccgca
     3121 tcgcggcccg gcgggggaac acggaggaag cgcagcggca gatcgccgcg gcacggcgcg
     3181 cgctggacgg cgacaccgcg atggcggcgc agcaggagcg attcttcccg tatctggtgg
     3241 gctacgtcgc cctgtacacg aacgatctgg ccacagccga agcacagctg accaagggcc
     3301 tcgcgctgcg cggcaacgag cgtgacccct tcctgcactg cctgctggcc atgacgctcg
     3361 agaaactggg acgccaggac gacgccaggg cggcgtatca gcgcgcgtac gacctggcga
     3421 cggcgcacaa cccgccggcg gcgttcgtgc ggcccttcgc gcgcaagaag ctcagcctgc
     3481 ccgcatcccg ctgacgccgt ggccatgccg gcggtcgagg cgctcgggcg actgcgcgag
     3541 ggcaacctcc ggttcgcctc cggcgcgagc gcgctgcagg acgcgctgag tcgcggccgg
     3601 cgcgccgagc tggccgagcg ccaggaaccg ttcgccatca tcctgggatg ctcggattcg
     3661 cgggtgccgg cggaaatcgt gttcgatcag ggcctcggcg acctgttcgt catccgcgtg
     3721 gccggcaaca tcgtcgcacc ttcccaggtg ggcagcatcg aattcgccgc cgcccggttc
     3781 ggcacgcagc tcgtcgtcgt gctgggccac tcccggtgtg gggccatcct ggcgacgctg
     3841 gaagagctgc ggactccgac ggaacgccag tcgcgtaatc tgcgcgcgat cgtggaccgc
     3901 gtgcgccctt ccgtggaagg attgctcgcc accgagctgc gtgacgatcc ggaagcgctg
     3961 gtgcaccagg ccgttcgcgc gaacatccgc gcctccgtgg accatctgcg gcacggctcc
     4021 gagctgctgg agcagttggc cctgcagggg cgcctgctcg tggtcggcgc tgagtactcg
     4081 ttggaaaccg ggttggtgga gttcttcgac gccgtgtgag caacgcgcgc ctgccagccc
     4141 gcgtcgggct ggcaagcgct catcgaaggt tcaatccagc agattcgccg ggaccttgcc
     4201 gccgttctcc accatcttgc ggaggacctg cttgtgcagc cagatgttca tggccgcggt
     4261 gtcctgcatg ttgcccgtgt agccgagctc cttggcgagc tctttccgct cggtcaggct
     4321 gctctccatg ccgacgagct tcatgaggtc cacgatcgag gtgcgccagt tgagcttctg
     4381 cgggttcttc gcggccatct gggtgagtac cgcttcgatg tcgaccacgg tggcgacggc
     4441 tgcgggcgcc ccggttgcgg tgggcgacgg cgcggcctca ctgcggccaa atatcttgga
     4501 catcaggttc cccaggatgc tcatacggta cctccaggtg tgtgtcgaaa gaccttcacg
     4561 cccggcaaac ccccgcctgg cgcgacatgg gagtgtacgt ctacccgcgt gaaggaccgc
     4621 gcaagtcggg acgaatttcc cttcccccgc cttgattgcc cgggattgtg cccgcacctt
     4681 ctgtagcatc
//
LOCUS       DBNGZW010000352         2311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1193513, whole genome shotgun sequence.
ACCESSION   DBNGZW010000352 DBNGZW010000000
VERSION     DBNGZW010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1193513"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="AC16JC_08320"
     CDS             <1..396
                     /locus_tag="AC16JC_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5164738.1"
                     /translation="EALRYAQKLLRHRAIIVVLSDFRAEGWEDPLARLAVRNDLVAIT
                     IDDPREVAFPHAGWVDFTDAETGQRLLLDTGHPGTRTRLRLAAERLVADRSRKLQQAG
                     ASRVALRTDVPYGVALRRAFHNRAHRLRR"
     gene            393..1235
                     /locus_tag="AC16JC_08325"
     CDS             393..1235
                     /locus_tag="AC16JC_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164739.1"
                     /translation="MTGILWAVVAGWVQGPAAAPEWTARSATAQATVGDTVWLERRFA
                     LPGGWRLRPGRLASDDEVEALADPVVLRDGDEWVVRYPVAAWTPGPHTVTLPPVWRLG
                     PDAQADSVSGGTASFVLASVIPDSLKHPEPRPALAPLRAQARDARLPALALLLAAAAL
                     VGGIRWRRRPPRVLPAPVAAVAGAGVPDRRWLEAGEPKAVAARAAGRLRTAIARAVPE
                     AHLGLPTSECLTVVQGRRPQAPVSELRTVFAALDEVAFAGAAEVDVVALARLAETLAG
                     RLGK"
     gene            1232..2212
                     /locus_tag="AC16JC_08330"
     CDS             1232..2212
                     /locus_tag="AC16JC_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR5164740.1"
                     /translation="MSFARPLLLVLLLALPLWWWLRSRRLGRLTGTPLSDARPAAAIR
                     QRLWLARVPVALRSFCVAAWIVAAAGPRIGAAKSEVRSEGISIVLAVDLSSSMLAEDF
                     TPANRLDVAKQTAIDFVRARESDRIGLVAFAGQALTQVPITNDHAVLEAALQDMHSGT
                     LEDGTAIGTGIATAANRLRRAPGKSRVIILLTDGVNNRGTVDPRTAARAASAFGIRIY
                     TIGVGTQGEAPVPTGQGGQGLRYQMLPVEIDEPLLTEVATMTGGRYFRATDSESLRHI
                     FAEINRLEKTTVQHIVYRRFDEAVGWPLSLGLLALGLELLLSATIVVRVP"
BASE COUNT          279 a          817 c          881 g          334 t
ORIGIN      
        1 gaggcgctgc gctatgcgca gaagctgctg cggcaccgcg ccatcatcgt ggtgctgtcg
       61 gacttccgcg ccgaaggttg ggaggatccg ctcgcgcgcc tcgcggtgcg caacgatctc
      121 gtggcgatca ccatcgacga tccccgggag gtcgcctttc cccacgccgg gtgggtggac
      181 ttcaccgacg ccgaaacggg acagcgcctg ctgctcgaca cgggccatcc cgggacgcgc
      241 acgcgactgc gcctggccgc ggagcggctc gtggccgatc gaagccgcaa actgcagcag
      301 gcgggggcgt cccgggtcgc gctccgcacc gacgtgccct acggcgtcgc cctgcggcgc
      361 gccttccaca accgggcgca ccgtctcaga cggtgaccgg gatcctctgg gcggtcgtcg
      421 ccggctgggt gcagggaccc gcggcggctc ccgagtggac ggcccggtcg gctacggccc
      481 aggcgacggt cggcgacacg gtgtggctcg aacggcggtt tgcgctgccg ggcggctggc
      541 gcctgcggcc gggccggctg gcgtcggatg acgaagtgga agcgctcgcg gaccccgtgg
      601 tgctgcgcga cggcgacgag tgggtggtgc gctatcccgt ggcggcgtgg acgcccggac
      661 cccacacggt gacgctgccg ccggtctgga ggctgggtcc cgatgcgcag gccgactccg
      721 tctcgggcgg caccgcgtcc ttcgtactcg ccagcgtgat tcccgacagc ctgaagcatc
      781 ccgagccgcg tccggcgctc gcgccgttgc gcgcccaggc gcgggatgca cgactgccgg
      841 cgctggcgct cctgttggcc gcggcggcgc tcgtcggcgg tatccggtgg cgtcgccggc
      901 caccgcgcgt gcttccggcg cccgtcgcag cggtggcggg cgcgggggtc ccggaccggc
      961 ggtggctcga ggccggagag cccaaggcgg tggccgcccg cgccgccggc cggctgcgca
     1021 cggcgatcgc gcgcgccgtg ccggaggcgc atctaggact gcccacgagc gagtgcctca
     1081 cggtcgtgca gggccgaagg ccgcaggcgc cagtatcgga gctgcggacc gtgtttgccg
     1141 cgctcgatga ggtcgcgttc gcgggcgccg ccgaagtcga cgtcgtcgcc ctggcgcggc
     1201 tcgccgagac tctggcggga cggctgggaa aatgagcttc gcgcgtccgc tgctgctggt
     1261 cctcctgctg gcgctgccgc tgtggtggtg gctgcggagc cgccggctcg gccgcctgac
     1321 gggcacgccg ctgtcggacg cgcggcccgc ggcggcgatc cggcagcggc tctggctcgc
     1381 ccgcgtgccg gtggcgctgc gcagcttctg tgtcgcggcg tggatcgtcg cggccgccgg
     1441 accgcgcatc ggagcggcga agtcggaggt gcggagcgag gggatctcga tcgtgctcgc
     1501 cgtggatctc tcgagcagta tgctggcgga ggatttcacg ccggccaacc ggctcgacgt
     1561 ggcgaagcag acggccatcg acttcgtgcg cgcgagggag tcggaccgca tcggcctggt
     1621 cgccttcgcc gggcaggcgt taacgcaggt tcccatcacg aacgatcacg cggtgctcga
     1681 ggccgcgctg caggacatgc acagcggcac gctcgaggac ggcaccgcca tcggtaccgg
     1741 gatcgccacc gcggccaacc ggctgcggcg cgcgccgggg aagagccgcg tcatcattct
     1801 gctcaccgac ggcgtgaaca accggggcac cgtcgacccc cgtaccgccg cccgcgccgc
     1861 ttcggcgttc ggcatccgca tctacaccat cggcgtgggg acgcagggcg aagcgccggt
     1921 gccgacggga cagggcgggc agggactccg ctaccagatg ctgccggtcg agatcgacga
     1981 gcccctgctc accgaggtcg cgacgatgac cgggggccgc tacttccggg cgaccgacag
     2041 cgagtcgctg cgtcatatct tcgccgagat caaccgcctg gagaagacga cggtgcagca
     2101 catcgtctac cgccggttcg acgaagcggt gggctggccg ctgtcgctgg ggctgctcgc
     2161 cctcgggctc gagctgctgc tgtcggcgac gatcgtggtg cgggtgccct gatgaccttc
     2221 gattcgccgg ccttgctgct gctcgccccc gtgatcggga ccgtgttctg ggtggccgcc
     2281 acctgggcgc gccgcgtgcg cctgtcgcac g
//
LOCUS       DBNGZW010000353         2956 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_374379, whole genome shotgun sequence.
ACCESSION   DBNGZW010000353 DBNGZW010000000
VERSION     DBNGZW010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2956)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2956)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2956
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_374379"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2074)
                     /locus_tag="AC16JC_08335"
     CDS             complement(<1..2074)
                     /locus_tag="AC16JC_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020248897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR5164741.1"
                     /translation="MVKRLFTRVFGTRFRREMKRIRPLVDAIHRHEARLKDLSDAELQ
                     AQTAKCRGIIAERTAELAAEVDRLKRAKHDCPDAEERARLSDQLGQAEQRYARALQAA
                     LDDILPEAYATVREACRRLQGTKVTVTAHEQAWDMVPYDVQLIGGVVLHQGKIAEMAT
                     GEGNTLVATLPLYLNALAGKGAHLVTVNNYLARRDSQWMGHLFTWLGLTVGCIDDTQP
                     ASPERKAAYRCDITYGTNNEFGFDYLRDNMVGRLDERVQRGHYYAIIDEVDSILIDEA
                     RTPLIISGAVGAESDVKYAQFNTQVVQLVRKQTAIVNDLIGKAEPLLADEKTQYEGAK
                     LLYQAQLGMPKNKKLLKLLNEQGIKTIVQRVELDRLADRKLPARDQKMRNVEDDLYFV
                     LDERGHSVHLTDKGVETMSPDDPTLFVVPDISHAVHVIDRDDTLTPAEKLERRRVVES
                     EYAGKSETLHIIHKLLQAHALYEKDVEYVVQDGQVFIVDEFTGRLMPGRRWAEGLHQA
                     VEAKEGVTVREESQTLATITIQNYFRMYEKLAGMTGTAETEETEFFQIYKLEVVVIPT
                     NRPVRRVDKHDLIYKTRREKYNAIVEETERQHKRGLPVLVGTVSVEVSETLSRMLKRR
                     GLKHEVLNAKYHEREAEIVAQAGQPGAITIATNMAGRGTDIKLGPGVKQCQVCGIRAR
                     GEPAFGQAV"
     gene            complement(2086..2646)
                     /gene="nadD"
                     /locus_tag="AC16JC_08340"
     CDS             complement(2086..2646)
                     /gene="nadD"
                     /locus_tag="AC16JC_08340"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HIR5164742.1"
                     /translation="MDALFGGSFDPVHLGHLVAAEAAAEELGARVRLMPVREQPFKHA
                     AHGATAEQRADMLALALAGNPRLSLERIELELAAPSYTVRTLRALGEREPGNRFTLLL
                     GADAARDLPAWFEVEALPALADVVVFGRPGAPVPDHPIIRRAIEVPAVDISATLVRRR
                     VAGGRSIRYLVPDAVREYIAAHGLYR"
     gene            complement(2651..>2956)
                     /gene="bamD"
                     /locus_tag="AC16JC_08345"
     CDS             complement(2651..>2956)
                     /gene="bamD"
                     /locus_tag="AC16JC_08345"
                     /inference="COORDINATES: protein motif:HMM:NF024915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR5164743.1"
                     /translation="IHVRDLRNWFADKAYKNGLFYFKRRAWDSGIIYFKDIVANYPEA
                     ARAPDALLRLVDTYRVIGYKEELQEACAHLRRFYPQAAGIAKSCPAERAAPPAPGSP"
BASE COUNT          423 a         1032 c          970 g          531 t
ORIGIN      
        1 ccacggcctg cccaaaggcc ggctcgccac gcgcccggat gccgcagacc tgacactgct
       61 tcacgccggg gccgagtttg atgtcggtgc cgcggcccgc catgttggtg gcgatcgtga
      121 tcgcgcccgg ctgcccggcc tgggccacga tctcggcctc gcgctcgtgg tacttggcat
      181 tcagcacttc gtgcttcagc ccgcgtcgct tgagcatccg ggagagcgtt tcggagacct
      241 cgacgctcac cgtgcccacc aggaccggca ggccgcgctt gtgctgccgc tccgtctcct
      301 cgacgatcgc gttgtacttt tcgcgccgcg tcttgtagat caggtcgtgc ttgtcgaccc
      361 ggcgcaccgg ccggttcgtc gggatgacca ccacttccag cttgtagatc tggaagaact
      421 cggtttcctc cgtctccgcc gtgcccgtca tgccggcgag cttctcgtac atgcggaagt
      481 agttctggat ggtgatggtg gcgagggtct gactctcctc gcgcaccgtc acgccttcct
      541 tcgcctcgac cgcctgatgc aggccttcgg cccagcgccg gccgggcatc agccggccgg
      601 tgaattcgtc cacgatgaag acctggccgt cctggacgac gtactccacg tccttctcgt
      661 acagggcatg ggcctggagc agcttgtgga tgatgtgcag cgtctcgctc ttgcccgcgt
      721 actccgactc caccacgcgc cgccgctcca gcttctcggc gggcgtgagg gtgtcgtcgc
      781 gatcaatgac gtgcaccgcg tgggagatgt cgggcacgac gaacagggtc ggatcgtcgg
      841 gcgacatcgt ctcgacgccc ttgtcggtga gatgcaccga atggccgcgc tcgtcgagca
      901 cgaaatagag gtcgtcctcg acgttgcgca ttttctggtc gcgggccggc agcttgcggt
      961 cggcgaggcg atcgagctcc acccgctgca cgatcgtctt gatgccctgc tcgttcagca
     1021 gcttcagcag cttcttgttc ttgggcatgc cgagctgcgc ctgatagagc agcttggccc
     1081 cttcgtactg ggtcttctca tccgccagca agggctccgc tttgccgatg agatcgttca
     1141 cgatcgcggt ctgcttgcgg accagctgca cgacctgcgt attgaattgc gcgtacttca
     1201 cgtccgattc ggcgcccacg gctcccgaga tgatgagcgg cgtgcgcgct tcgtcgatca
     1261 gaatcgagtc gacctcgtcg atgatcgcgt agtagtgccc gcgctgcacg cgctcgtcga
     1321 gccggccgac catgttgtcg cgcaggtagt cgaacccgaa ctcgttgttc gtcccgtagg
     1381 tgatgtcgca gcggtaggcc gccttccgct cgggcgaagc gggctgcgta tcgtcgatgc
     1441 acccgacggt gaggcccagc caggtgaaca ggtggcccat ccactgggag tcgcgccgcg
     1501 ccaggtagtt gttcaccgtg acgaggtgcg cgcccttccc ggccagtgca ttcagataga
     1561 gcggcagcgt ggcgaccagc gtgttccctt ccccggtggc catctccgca atcttgccct
     1621 ggtgcagcac gacgccgccg atcagctgga cgtcgtaggg caccatgtcc caggcttgct
     1681 cgtgcgcggt caccgtgacc ttcgtgccct gcaaccgccg gcacgcctcc cgcacggtgg
     1741 cgtaggcctc gggcaggatg tcgtccagcg cggcctgcag cgcgcgcgca tagcgctgct
     1801 cggcctggcc cagctgatcg gacagccgcg cccgctcttc cgcgtcgggg cagtcgtgct
     1861 tggcccgctt caggcggtcc acctccgcgg ccagctccgc cgtgcgctcg gcgatgatcc
     1921 cccggcactt ggcggtctgg gcctggagct ccgcatcgct caggtccttg agccgcgctt
     1981 cgtgccggtg gatcgcgtcg accagcgggc ggatgcgctt catctcccgc cggaaccgcg
     2041 tcccgaacac cctggtaaac agccgtttga ccatcggtcc tcagtctagc gatacagccc
     2101 gtgcgcggcg atgtattcgc gcaccgcgtc gggcaccagg taacgaatgg accggccccc
     2161 ggccacccgg cgccgcacca gcgtcgccga aatgtccacg gccggcacct cgatcgcccg
     2221 gcggatgatg ggatggtccg gcacgggagc acccggccgg ccgaacacga ccacgtccgc
     2281 cagggcgggc agcgcctcca cctcgaacca ggccggcagg tcccgggcgg catccgcccc
     2341 cagcaataac gtgaaccggt tccccggctc gcgctccccc agggcccgca gggtccggac
     2401 ggtgtaggac ggcgcggcca gctcgagctc gatccgctcc agggagaggc gcggattgcc
     2461 ggcgagcgcc agggccagca tgtcggcgcg ctgctcggcg gtcgccccgt gggccgcgtg
     2521 cttgaagggc tgctcgcgca ccggcatcag acgaacgcgc gctcccagct cctccgccgc
     2581 tgcctcggcg gccacgagat ggccgagatg gaccggatcg aacgatccgc cgaacaacgc
     2641 gtccactcgc tcagggagaa ccgggtgcgg gaggagcggc gcgttccgcc gggcaggact
     2701 tggcgatgcc cgccgcctgc ggatagaagc ggcgcaggtg ggcacacgcc tcctgcagct
     2761 cttccttgta gccgatcacg cggtaggtgt ccacgagccg cagcagggca tcgggggccc
     2821 gcgccgcttc cgggtagttg gccacgatgt ccttgaaata gatgatgccg gagtcccagg
     2881 cgcgccgctt gaagtagaac aggccgttct tgtacgcctt gtcggcgaac caattgcgca
     2941 agtcgcgcac gtggat
//
LOCUS       DBNGZW010000354         5752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_169833, whole genome shotgun sequence.
ACCESSION   DBNGZW010000354 DBNGZW010000000
VERSION     DBNGZW010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5752
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_169833"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1538
                     /locus_tag="AC16JC_08350"
     CDS             <1..1538
                     /locus_tag="AC16JC_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5164744.1"
                     /translation="TSCHSQIVQGQHVAVTDATCFLCHFKDRPPGAPIAGCTGCHPSP
                     PRIESPAGYVVDHAQYVNDRISCISCHSEVTAGSGVAERSRCFNCHNEPARLNQFDNT
                     TLVHQVHIAEHKVECTQCHTPIEHRVVALTSTFELDCRSCHAQVHDAQRRLYAGMGGH
                     GTPESPSSMFKARVSCLGCHAQPKTVRGHERVQLAGEASCLSCHGVRYANMLPAWQRE
                     MEQKVSRVAPVVAAARTALGAVPLGRRAVADSLLRQAQENVDLVRVGKAAHNVLFADN
                     LLRASLDLVREAVAAGKLPYRVPSLSLGPPLGENACFRCHVGVERSTVPFQGGTFAHA
                     PHVLRGGLGCGACHTPLENHGGTLITSRAACDACHHDPVRPTNCAVCHGGAGGGGVPD
                     TTLRLATGDFSHGVHRDAGLACTLCHTPPAMSARGLDCDNCHDQHHQPDRTCLSCHRG
                     GVLDQHTRAVHIACVECHEGAATLKRWSPQICTTCHVQQATGHNPGKPCEACHKVPAM
                     QRG"
     gene            1546..2814
                     /locus_tag="AC16JC_08355"
     CDS             1546..2814
                     /locus_tag="AC16JC_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164745.1"
                     /translation="MSSRTFTRLLGLLAAGVSLGFGAVHGQASQVVQCEKCHASRDFI
                     TGKAGGPQDTSLYVPASILQGTRHSGLRCPDCHRGYEAGYPHRVATRVVPCQTCHEAE
                     GRDWQASIHAANAVSQGDAPTCVRCHGSHVVYGADDRRSPVYSLNVAALCGSCHADPR
                     IIGTYFATPGKAQARTAVEQYFKTVHGNALTRDGLVVSATCNDCHGAHKVLPADSAES
                     SVNRRNIPATCGACHVGVAEVYERSSHGQAYLSGRKNNEGHPAPVCVDCHTAHGIVRA
                     DQPQWLLGVVAECGSCHERLYETYFETYHGKVTRLGFTLAATCSDCHTAHDMRPASDP
                     RSSVFPANRVATCARCHPAANENFARYRPHGDPKDRARYPVLFWTWLFMTALLAGVML
                     FFGTHTTLWLVRIILNRLRGAAPEPPGTGP"
     gene            2811..3662
                     /locus_tag="AC16JC_08360"
     CDS             2811..3662
                     /locus_tag="AC16JC_08360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0045333 - cellular respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HIR5164746.1"
                     /translation="MTPQTLDAASRGEQPYLWRFTPLQRTLHALVIVSFLGLVITGLP
                     LRFAYAPWARVLVRALGGLEAAGVIHRLCAIITFGYFAIHVGSLIYRVAKGTSWKKIL
                     WGPDSMVPQGKDVRDLIGMFKWFLGLGPRPRFERYSYMEKFDYLAVFWGVAIIGFSGL
                     LLWFPEFFSRFLPGWVFNVATIIHGDEALLAMSFIFTIHFFNVHLRPEKFPIDLVIFT
                     GRATTDYLKDEHPLEYERRRDAGALDGLSAPPVSRLAYVWSVVLGFASLSLGIFVIIL
                     VLWAVLH"
     gene            3692..5170
                     /locus_tag="AC16JC_08365"
     CDS             3692..5170
                     /locus_tag="AC16JC_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164747.1"
                     /translation="MTLLLLVSPSRAPAQQYRIRLDTRAQQVSYRGVTLDSIPVTDTV
                     STPGRGPTSPDGFAVLCLSGVPYCTFFRPGPWRNGGPLTSTVDLTVWGLGVSGLSVHA
                     AGRLGVEFGSADVWPGTDPAVQLLEGYAEYAATRATARLGRQVVASRLGTTGFDGAGV
                     VLRDARRGVEIDGYAGWGLARGVALPVTSPVLNPLDDFQPRQRQLVAGAGAGWTKGLA
                     DLRLDYLREVDPGTDHFVSERVGLGATLRPIPGVQLAGGADYDIAAGWWGSAEGTLTY
                     AQSKVQASVGARRYRPHFDLWTIWGAFSPVPYHAVQASVAVTAIRGLALRGRWEGYEF
                     EAAETATPLFNAKRDGWRWELGGTYQPLSSLAVDASYRQEYGPGAGVAAVAGTITYEP
                     SRRLTVVLLGSQATRPLEFRFNEAEVRVFGLDAQFEPSSRVRLGAAADRYREIHRRPD
                     AGAFDWNQWRVSGRVVLLFGSPADLRGLPPSIRLLPGGRAER"
     gene            5167..>5752
                     /locus_tag="AC16JC_08370"
     CDS             5167..>5752
                     /locus_tag="AC16JC_08370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5164748.1"
                     /translation="MNSRAPVLALAVIASVMAVSRLRAQQPPRFDHLKHASLFPTCTG
                     CHQGAEDPEQPILPDSAACASCHDGTTRPYVAWRRPTEAHPSNLKFAHELVPLMTRET
                     PQGTQTLSCEDCHVAPGGQRMAVRRATPEGCLACHGRGVTQHLAAPDSLCQTCHLPLV
                     RAAALRPADVAGFPAPPSHQAPNFVRRAGHGALAK"
BASE COUNT          826 a         2023 c         1972 g          931 t
ORIGIN      
        1 gtacgagctg ccactcccag atcgtgcagg ggcaacacgt cgccgtgacc gacgccacgt
       61 gttttctctg tcacttcaag gatcgcccgc cgggcgcgcc gatcgccggg tgcacggggt
      121 gtcacccatc cccgccccgc atcgagtccc cggccgggta cgtggtcgac cacgcccagt
      181 acgtcaatga ccgcatctcc tgcatctcgt gtcacagcga ggtgacggcg ggctccggcg
      241 tggccgagcg ctcgcgctgc ttcaactgcc acaacgagcc ggcccggctc aaccagttcg
      301 acaacacgac gctcgtccat caggtgcata tcgccgagca caaggtggag tgcacccagt
      361 gccacacccc gatcgaacac cgggtcgtcg ccctgacctc gacgttcgag ctggactgcc
      421 gtagctgtca cgcacaggtc catgacgcgc aacggcgtct ctatgccggg atgggggggc
      481 acggcacccc ggaatcgccg agcagcatgt tcaaggcccg cgtctcgtgc ctcggctgcc
      541 acgcgcagcc caagaccgtg cgggggcacg agcgcgtgca gctcgccggc gaggcctcct
      601 gtctctcgtg ccacggcgtt cgctacgcga acatgctgcc tgcgtggcag cgggaaatgg
      661 agcagaaggt cagccgcgtc gcaccggtcg tcgcagccgc ccgcaccgcg ctcggggcgg
      721 tcccgctcgg acggcgagcc gtcgccgaca gcctgctccg ccaggcgcag gaaaacgtcg
      781 acttggtgcg ggtcggcaag gccgcgcaca acgtgctgtt cgccgacaat ctgctgcggg
      841 cgtccctgga tctcgtgcgg gaggcggtgg cggccgggaa gctgccgtac cgcgtgcctt
      901 cgctgagcct gggaccgccc ctgggcgaga acgcctgttt ccggtgccac gtcggggtgg
      961 aacggtcgac cgtgccgttc cagggcggca ccttcgcgca cgcgccgcac gtactgcgcg
     1021 gcggcctcgg atgcggtgcc tgccatacgc cgctcgagaa tcacggcggg acgctgatca
     1081 cctcgcgggc ggcgtgcgac gcgtgtcatc acgacccagt gcggccgacg aactgcgcgg
     1141 tgtgccacgg cggcgccggc gggggcgggg ttccggatac gacgctgcgg ctggccaccg
     1201 gggacttctc gcacggcgtc caccgcgacg cgggtctggc gtgcaccctc tgccacactc
     1261 cgcccgccat gagcgcgcgt ggcctcgact gtgacaactg tcacgatcag catcaccagc
     1321 ccgaccggac gtgtctgtcc tgccatcgcg gcggggtgct ggatcagcac acgcgagcgg
     1381 tgcacatcgc ctgcgtggag tgccacgagg gggcggcgac gctcaagcgc tggtcaccgc
     1441 agatctgcac gacgtgccat gtgcaacagg ccaccggtca caatcccggc aagccgtgcg
     1501 aggcctgcca caaggtgccg gccatgcaac gcggatagcg ggatcatgtc ctcacgaacg
     1561 ttcacccgat tgctggggct cctggccgcc ggtgtatcgc tgggattcgg ggcggtccac
     1621 ggccaggctt cgcaggtcgt gcagtgcgag aaatgccatg ccagccggga cttcatcacc
     1681 ggcaaggcag ggggaccgca ggacacgtcg ctgtacgtgc ccgcgtcgat cctgcagggt
     1741 acgcggcaca gcggcctgcg gtgccccgac tgccaccggg gctacgaggc cgggtatccg
     1801 caccgcgtcg ccacccgggt ggtgccgtgc cagacctgcc atgaggccga agggcgggac
     1861 tggcaggcgt cgatccacgc cgcgaacgcg gtgtcgcagg gagacgcccc gacctgcgtg
     1921 cgctgtcatg gctcgcacgt cgtgtacggg gcggatgatc gtcgctcccc ggtctattcg
     1981 ctgaacgtcg ccgccctgtg cggcagctgc cacgccgatc cccggatcat cggaacgtac
     2041 ttcgcgaccc cgggcaaggc ccaggcgcgc acggccgtcg agcagtactt caagaccgtt
     2101 cacggcaatg ccctgacgcg cgacgggctg gtcgtttcgg cgacctgcaa cgactgccat
     2161 ggggcgcaca aggtcctacc ggcggattcc gcggagtcgt cggtgaaccg gcggaacatc
     2221 ccggcgacct gcggagcctg ccacgtcggc gtcgccgagg tgtacgagcg ctcctcccac
     2281 ggtcaggcgt acctcagcgg gcgcaagaac aacgagggcc acccggcccc cgtgtgcgtg
     2341 gattgccaca cggcccacgg gatcgtgcgt gccgaccagc cgcagtggct gctcggggtc
     2401 gtggcggaat gcggcagctg tcacgagcgg ctctacgaga cgtacttcga gacgtatcac
     2461 ggcaaggtga cccgtctggg gttcacgctc gcggcgacgt gcagcgactg ccacacggcg
     2521 cacgacatgc gtcccgcgtc cgacccacgc tcgtcggtct tcccggcgaa ccgtgtcgcg
     2581 acgtgcgcca ggtgccaccc ggcggccaac gagaacttcg cgcgctatcg cccccatggc
     2641 gatcccaagg accgcgcgag gtacccggtg ctgttctgga cctggctgtt catgaccgcg
     2701 ctgctggccg gcgtgatgct cttcttcggg acccacacga ctctctggct ggtgcgcatc
     2761 atcctcaacc gcctccgcgg tgcggcccct gaacctcccg gtaccggacc atgacaccac
     2821 aaaccctgga cgctgccagc cgcggcgagc agccctatct ctggcggttc acgccgctgc
     2881 agcgcacgct gcacgccctg gtgatcgtct cgttcctcgg tctcgtcatc acgggcctgc
     2941 cgctgcgctt cgcctacgcg ccctgggccc gggtgctggt ccgcgcgttg ggcggcctcg
     3001 aggcggcggg cgtcatccac cggctgtgcg ccatcatcac gttcggctat ttcgcgattc
     3061 acgtgggctc cctgatctac cgcgtggcca aggggacctc ctggaagaag atcctctggg
     3121 gacccgactc catggttccg caaggcaaag acgtgcggga cctgatcggg atgttcaagt
     3181 ggttcctggg cctgggcccg cggccccggt tcgagcgcta cagctacatg gagaagttcg
     3241 actacctggc ggtgttctgg ggggtcgcca tcatcgggtt ctccggcctg ctgctgtggt
     3301 ttcccgagtt cttctcgcga ttcctccccg gctgggtctt caacgtggcc acgatcattc
     3361 acggggatga agcgctgctc gcgatgagct tcattttcac gattcacttc ttcaacgtgc
     3421 atctgcgccc ggagaagttc ccgatcgatc tcgtgatttt caccgggcgc gccaccaccg
     3481 actacctgaa ggacgagcat ccactcgagt acgagcgccg gcgtgacgcg ggtgccctcg
     3541 acgggctgag cgcccctccg gtgtcccgtc tcgcgtacgt ctggtccgtc gtcctgggct
     3601 tcgcctcgct gtccctcggg atcttcgtca tcattctcgt tctctgggcg gtactccatt
     3661 gaacccccgt ctcccattcg ttgcggcggg cgtgacgctg ctgctcctgg tgagcccgag
     3721 ccgcgccccg gcgcagcagt accggattcg tctcgacacg cgggcgcagc aggtttccta
     3781 tcgcggcgtc acgctcgaca gcatccccgt caccgatacc gtctcgaccc cggggcgggg
     3841 accgacctcg cccgacggct tcgccgtgct gtgcctgagc ggcgtaccct actgcacgtt
     3901 cttccggccc ggtccctggc gtaacggcgg cccgctgacc agcacggtcg acctgaccgt
     3961 ttggggactg ggcgtgtccg gcctgagcgt gcacgcggcc gggcggctgg gcgtcgagtt
     4021 cggctcggcc gatgtctggc ccgggaccga cccggcggtc cagctgctgg agggctacgc
     4081 ggaatacgcc gcgacccgcg caacggcgcg attgggccgg caggtcgtcg cgtcccgcct
     4141 gggcacgacc ggattcgacg gtgccggcgt ggtgctgcgc gacgcgcggc gcggcgtgga
     4201 gatcgacggc tacgcgggct gggggttggc ccgcggcgtc gcgctgccgg tcaccagccc
     4261 cgtgctcaat ccgctcgacg atttccagcc gcgacagcgc cagctcgtcg ccggtgcggg
     4321 tgcaggctgg acgaagggcc tggcggacct tcggctggac tacctgcggg aagtggaccc
     4381 cgggacggac cacttcgtct cggaacgggt gggtctgggg gccacgctgc ggccgattcc
     4441 gggagtccag ctcgccggcg gggccgacta cgacatcgca gccggatggt ggggcagcgc
     4501 cgaggggacg ctcacctacg cacagtccaa ggtgcaggcg agcgtcggcg cccgtcgtta
     4561 ccggccgcat ttcgatctgt ggaccatctg gggcgcgttc agccccgtgc cgtatcacgc
     4621 cgttcaggcc tccgtggccg tcacggcgat tcgcgggctc gcgttgcggg gccggtggga
     4681 agggtacgaa ttcgaagcgg cggagacggc gacgccgctg ttcaatgcga agcgcgacgg
     4741 ctggcgttgg gagttgggcg gcacctacca gcccctcagc agcctggcag tggatgcatc
     4801 ctaccgccag gagtacgggc cgggggcggg agtcgccgcg gtggccggga ccatcaccta
     4861 cgagccttcc cggcggctca cggtcgtact gctcggatcg caggccacgc gtcccctgga
     4921 gttccgcttc aatgaagccg aagtacgggt gttcggactg gacgcgcagt tcgagcccag
     4981 ctcacgcgtg cggctcggcg cagcggccga ccgctatcgg gagatacatc gccggcccga
     5041 cgcgggcgcg ttcgactgga atcaatggcg cgtcagcggg cgcgtggtgc tgttgttcgg
     5101 gagccccgcc gatctgcgcg gcctgccgcc atccatcaga ctgctgccgg gggggagggc
     5161 ggaacgatga actctcgagc cccggtcctc gccctggcgg tcatcgcgag cgtaatggcc
     5221 gttagcaggc tgcgcgctca gcagccgcca cgcttcgacc atctgaagca tgccagcctg
     5281 tttcccacct gcaccgggtg tcaccagggg gccgaggatc ccgagcagcc gatccttccc
     5341 gattcggcgg cctgtgcgtc ctgtcacgac ggcacgacac gcccgtacgt cgcgtggcgg
     5401 aggcccacgg aagcccatcc cagcaatctg aagttcgccc acgagctggt tcccctgatg
     5461 accagggaga cgccgcaggg gacgcagacg ctgtcgtgcg aggactgcca tgtcgccccg
     5521 ggggggcagc ggatggcggt gcgtcgggcc accccggagg ggtgcctggc ctgccacggg
     5581 cgcggggtga cgcagcatct cgcggctccg gacagcctgt gtcagacgtg ccatctaccc
     5641 ctggtgcgcg ccgccgcact ccggcccgct gacgtggccg gattccccgc gccgccttcc
     5701 catcaggctc cgaacttcgt cagacgtgcg ggccacggcg ccctggcgaa gg
//
LOCUS       DBNGZW010000355         3420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_850820, whole genome shotgun sequence.
ACCESSION   DBNGZW010000355 DBNGZW010000000
VERSION     DBNGZW010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_850820"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(125..685)
                     /gene="rplO"
                     /locus_tag="AC16JC_08375"
     CDS             complement(125..685)
                     /gene="rplO"
                     /locus_tag="AC16JC_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HIR5164749.1"
                     /translation="MAKAKGKTKAKPARRAARAAPRRKAAAQAAESSVEPRPITLSTL
                     RPPRGSTHARLRVGRGPGSGIGKTSGRGQKGQRARAGGTVRPGFEGGQMPLIRRIPKR
                     GFTNPFREPAQVVNVRHLGLLAEGAEVTPQTLFGAGLVRRPDTPIKLLGMGDVQRRFT
                     VKGVGVSASARQKIEAAGGTIEGPSA"
     gene            complement(692..952)
                     /gene="rpmD"
                     /locus_tag="AC16JC_08380"
     CDS             complement(692..952)
                     /gene="rpmD"
                     /locus_tag="AC16JC_08380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HIR5164750.1"
                     /translation="MGRLPPTRKYRKRKRSTDPVLPPVGKGKLEIRQTRSGSGKPARV
                     KRTLEALGLKHHQDVVVHADHPALRGMLYQVRHLVRVTPVKG"
     gene            complement(945..1676)
                     /gene="rpsE"
                     /locus_tag="AC16JC_08385"
     CDS             complement(945..1676)
                     /gene="rpsE"
                     /locus_tag="AC16JC_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019481509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HIR5164751.1"
                     /translation="MMDEQLPGAQAPETAPPPPAPETAAAAVESAPPPAPEHHRRGGR
                     GGRGGGGGRGGHGHGRGRGGERGPGGDRGREGDGMVENVIAINRVAKVVKGGRRFSFN
                     ALVAVGDGQGHVGVATGKANEVSEAVRKAVEAARRKTAEVPLTGGTIPHEIIGQHGAG
                     RVLLKPAASGTGVIAGGPVRAVLECAGVRDILTKSLGSTNPHNMVRATMDGLTHLTTA
                     RRVAKERGVEVEAIHYKARQEATRG"
     gene            complement(1677..2048)
                     /gene="rplR"
                     /locus_tag="AC16JC_08390"
     CDS             complement(1677..2048)
                     /gene="rplR"
                     /locus_tag="AC16JC_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HIR5164752.1"
                     /translation="MRAIRKIKTQADRRARRHLRVRKRINGTAARPRLVVFRSSKHIY
                     AQLVDDERGVTLVGAADTTEGIQVEGKGKTAKSFALGRLIAARAKDKGIAKVVFDRGG
                     YQYHGRVKAVAEGARKGGLEL"
     gene            complement(2051..2596)
                     /gene="rplF"
                     /locus_tag="AC16JC_08395"
     CDS             complement(2051..2596)
                     /gene="rplF"
                     /locus_tag="AC16JC_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009782735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HIR5164753.1"
                     /translation="MSRIGKRPVPLPKGVTATIAGRTITVKGPKGEISRTMHPDLTLA
                     LEDDTVAVRRPSDEPRHKALHGLTRTLVANMVEGVTHGFSRALEIQGVGYKAEVKPFG
                     VQLVVGYSHPVPYHAPKGITITVENNTQVKVQGVDKELVGQVAAELRSVRPPEPYKGK
                     GIRYVGEQVRRKAGKTAQAAK"
     gene            complement(2606..3001)
                     /gene="rpsH"
                     /locus_tag="AC16JC_08400"
     CDS             complement(2606..3001)
                     /gene="rpsH"
                     /locus_tag="AC16JC_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012525981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HIR5164754.1"
                     /translation="MSMTDPVADMLTRVRNACTAGHRRVDMPVSKLKVEIARILRDNH
                     YIQDFKVLDDGRFGLLRLYLKYHNDAPVIRELVRVSAPGLRRYVKAREIPRVKNGLGM
                     AILSTPQGVMSDRDARTARVGGELLAVVW"
     gene            complement(3014..3199)
                     /locus_tag="AC16JC_08405"
     CDS             complement(3014..3199)
                     /locus_tag="AC16JC_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008633399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HIR5164755.1"
                     /translation="MAKKCWIERVKRTPKFKVRAYQRCQRCGRSRGTLRKFGLCRICF
                     RELALRGEIPGVRKASW"
     gene            complement(3211..>3420)
                     /locus_tag="AC16JC_08410"
     CDS             complement(3211..>3420)
                     /locus_tag="AC16JC_08410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164756.1"
                     /translation="PTKSFDGRGNYTLGIKEQMVFPEIDYDKVEQIHGMDITFVTNAG
                     RDDTAMALLREMGMPFRGESPVAVG"
BASE COUNT          416 a         1304 c         1112 g          588 t
ORIGIN      
        1 gcgaggttca cccccggcgt cgcgatgtgc gcgccgatgc ggtagatgag caggcacagg
       61 agcgtgaaga ggatcttctc cttcagctcc ggcaccttga acaggtttgg gatccgcggg
      121 gcggtcacgc gctcggtccc tcgatggtgc cgccggccgc ctcgatcttc tgccgcgccg
      181 aggctgagac cccgacgccc ttcaccgtga agcggcgctg cacgtccccc atgcccagca
      241 gcttgatcgg cgtgtcgggc cggcgcacca gccccgcccc gaacagcgtc tgcggcgtca
      301 cttcggcgcc ctcggcgagc aaccccaggt ggcgcacgtt gacgacctgc gccggctccc
      361 ggaacgggtt ggtgaacccg cgcttgggaa tgcgccggat cagcggcatc tgcccgccct
      421 cgaaccccgg ccgcacggtg ccgccggccc gggcccgctg gcctttctgg ccgcgtcccg
      481 aggttttgcc gatgccggag cctggcccac ggcccacgcg caagcgcgcg tgggtggacc
      541 cgcggggcgg ccgcaacgtg gacagcgtga tcgggcgcgg ctccacgctg ctctctgccg
      601 cctgcgccgc cgctttgcgc cgcggcgcgg cccgcgcggc ccgccgggcg ggcttcgctt
      661 tcgtcttccc cttcgccttg gccatcgtcc cttatccctt gacgggcgtc acccgtacga
      721 ggtggcgcac ctggtacagc atgccgcgca gcgccggatg atcggcgtgc acgaccacgt
      781 cctggtggtg cttcaggccg agcgcctcga gcgtgcgctt cacccgcgcc ggcttccccg
      841 agcccgatcg cgtctggcgg atctcgagct tgcccttccc gaccggcggc agcaccggat
      901 cggtgctgcg cttccgtttg cggtatttcc tcgtcggcgg cagcctaccc acgggtcgcc
      961 tcctggcggg ccttgtagtg gatcgcctcg acttccacgc cgcgctcctt ggccacccga
     1021 cgcgccgtcg tgagatgggt gaggccgtcc atcgtggccc gcaccatgtt gtgcggattg
     1081 gtggacccga ggctcttggt caggatgtcg cgcacgccgg cgcattcgag cactgcgcgc
     1141 accgggccgc cggcgatgac gccggtgccg ctcgccgcgg gcttgagcag cacgcgcccc
     1201 gcgccgtgct gcccgatgat ctcgtgcggg atcgtgccgc cggtgagcgg gacttccgcg
     1261 gtcttgcgcc gggccgcttc caccgccttg cgcaccgcct cgctgacttc gttggccttg
     1321 ccggtggcga cgccgacgtg cccctgtccg tcgcccaccg cgacgagggc gttgaagctg
     1381 aagcgccgcc cgcccttcac caccttggcc acgcgattga tggcgatgac gttctccacc
     1441 atgccgtcgc cttcgcgacc tcggtcgccg cccgggcccc gctcgccgcc gcggccgcgc
     1501 ccatgcccgt gaccaccacg cccgccgcca ccgccgcgac cgccgcgccc gccccgccgg
     1561 tgatgctcgg gtgccggggg gggggccgac tcgaccgcgg ccgccgccgt ctcgggagcg
     1621 ggaggaggag gcgccgtctc gggcgcctgc gcgcccggca gttgttcatc catcatctag
     1681 agctccaatc cgcctttacg ggcgccctcg gccaccgcct tgacgcgccc gtggtactgg
     1741 tacccgccgc gatcgaacac gaccttggcg atccccttgt ccttggcccg ggcggcgatc
     1801 agcctcccca gcgcgaagct cttcgccgtc ttgcccttgc cttccacctg gattccctcg
     1861 gtcgtgtcgg ccgcgcccac cagggtcacc ccgcgctcgt cgtccaccag ctgggcgtag
     1921 atgtgcttgg aggagcggaa caccaccagc cggggccgcg ccgcggtgcc gttgatgcgc
     1981 ttgcggaccc gcaggtgccg gcgcgcgcgc cggtcggcct gcgtcttgat cttgcgaatg
     2041 gcgcgcatgg ctacttcgcc gcctgggcgg tcttgcccgc cttgcgccgc acctgctcgc
     2101 ccacgtagcg gatccccttc cccttgtacg gctccggcgg acgcacgctg cgcagctcgg
     2161 ccgcgacctg tcccaccagc tccttgtcca ccccctggac cttcacctgc gtgttgttct
     2221 ccacggtgat cgtgatgccc ttcggggcgt gatacggcac cggatgcgag tagcccacga
     2281 cgagctgcac gccgaacggc ttgacctcgg ccttgtagcc gacgccctgg atctcgaggg
     2341 cccggctgaa gccgtgggtg acgccttcga ccatgttcgc caccagcgtg cgggtgagcc
     2401 cgtgcaacgc cttgtggcgc ggctcgtcgg aggggcgccg caccgcgacc gtgtcgtcct
     2461 cgagcgcgag cgtcaggtcc gggtgcatcg tccggctgat ctcgcccttg ggacccttca
     2521 ccgtgatcgt ccgtcccgcg atcgtcgccg tcacgccctt ggggagcgga accggacgtt
     2581 tgccgatacg cgacatggcc gcctcctacc agaccaccgc gagcagctcg ccgcccaccc
     2641 gggcggtccg cgcgtcgcgg tccgacatca cgccctgcgg cgtgctgagg atcgccatcc
     2701 ccagtccgtt cttcacccgc gggatctcgc gcgccttcac gtagcgccgc aggcccgggg
     2761 ccgagacgcg caccagctcg cgaatcaccg gggcgtcgtt gtggtacttg agatagaggc
     2821 gcagcagacc gaagcggcca tcgtccagga ccttgaagtc ctggatgtaa tggttgtcgc
     2881 gcaggatccg ggcgatctcc actttcagct tcgacaccgg catgtcgacc cggcggtgcc
     2941 ccgccgtaca ggcgttgcgc acccgggtga gcatgtcggc gacgggatca gtcatactca
     3001 ttgcgcctcc cgcctaccag ctggctttgc ggacgccggg aatttccccg cgcagcgcca
     3061 gctcacggaa acaaatgcga cacagcccga acttacgcag cgtgccccgg ctgcggccgc
     3121 agcgctgaca gcgctgatac gcccgcacct tgaacttcgg ggtgcgcttg actcgctcga
     3181 tccagcactt tttcgccatg ccgtcctctc ctagccgacc gcgaccgggg attcgccgcg
     3241 gaacggcatc cccatctcgc gcaacagcgc catggccgtg tcgtcccggc cggcgttcgt
     3301 gacgaacgtg atgtccatcc cgtggatctg ctcgaccttg tcgtagtcga tctccgggaa
     3361 gaccatctgc tccttgatgc ccagcgtgta attgccccgg ccgtcgaagc tcttggtcgg
//
LOCUS       DBNGZW010000356         4163 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1533266, whole genome shotgun sequence.
ACCESSION   DBNGZW010000356 DBNGZW010000000
VERSION     DBNGZW010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4163
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1533266"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1629
                     /locus_tag="AC16JC_08415"
     CDS             <1..1629
                     /locus_tag="AC16JC_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR5164757.1"
                     /translation="SDVDGDGDQDLLVADSAGVHLLRNDGGNANLAMQVQLMALRTGS
                     GKNNNFGIGATIEVRAGELFQTRVVTGRLTPFGLGPHLKADVLRVQWTNGVPETVYFP
                     GTDQDVLELEQLKGSCAFLYTWDGKRFRFVTDVMWRSALGMPLGIMGGGRGAAYAPAG
                     ASQEYLRIPGDALQLRQGRYVLQFTEELWETAYLDEIRLLAVDHPDSVDVYVDERFVP
                     PGPVQLRLFQAVHRRPPRSAVDDRGEDVLPALRERDDVYVSNLTPSRYQGLVEPHDLI
                     LDLDDDAGRPGTFLFLRGWIYPTDASINVALSQQTRLSTQLPALDVRDGHGRWTTALP
                     NLGFPSGKDKTIVVDLAGIFPTKDHHVRIRTNMQIYWDQAFVARDAPASPSRITPLPA
                     ESADLHFRGFSRTYRRGGRYGPHWFAYDDVSTDSPWRPIAGAVTRFGTVLPLLGSADD
                     MYAIMAPGDEVTVQFDAHSARMPPPGWRRTFLLYTDGWIKDADLNTAFGNTVEPLPFH
                     AIRNYPYAPGEAYPTDPAHQRYRREYNSRVVTPR"
     gene            complement(1616..3250)
                     /locus_tag="AC16JC_08420"
     CDS             complement(1616..3250)
                     /locus_tag="AC16JC_08420"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5164758.1"
                     /translation="MTQRCTIAVVGSGFAGSLLARVLAVLGHDVVLLERGAHPRFAIG
                     ESTTPLANLSMERLARRYGLTDCYRLAAHGRWLEHFPHLRRGLKRGFTFYRHHPGEPF
                     ADRGFASERLLVAASPTDAVADMHWLRADVDHHFVREAVAAGVEFRDRVELTGVAIDA
                     NGVRLTGQHAGAGLDLRVQFVIDASGPGGFLARQLPIPSGLRPTKTRSALVFSHFSGV
                     APMADVVPELPAGPYPDDWAAVHHLIDGGWMYALRFDHGVTSAGFALSPRGLAAVSAS
                     PTPDPEVLWRRLLDRYPTIGAAFGEATPLMPLVFRSRIQHRLTRAAGERWARLPHAYA
                     VVDPLFSTGIAWSLRAIERLALCFERGTRGSGVPNADDLGRNAELLRAEANQIDRLVA
                     GAYEAMAHFDLFAAHAMIYFTAVSFAEAHQRLVHRDSETAAWSGFLGIGDALLEPLPR
                     ESHRRLRRITRGRGRRGTLGQRRAFAAWAAAAIEPRNIGGFADPAAHHLYPVDVDRLV
                     DRHALLGLSRDELVSVLPALRGMAPAFRGEEPLSAV"
     gene            complement(3247..>4163)
                     /locus_tag="AC16JC_08425"
     CDS             complement(3247..>4163)
                     /locus_tag="AC16JC_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5164759.1"
                     /translation="SLRPPRRQVDPWIAHTSILEHERSPSGALERSLTVFLAGAECPF
                     TCSFCDLWRWTVDGPTPPGALPAQLARVLLALERPVPDRLKLYNASNFFDRRAVPAVD
                     VPALAQLAHPFAAVTVESHASTIGPLTLEFARLIPGRLEVAVGLETIHPVAARHLNKR
                     LGPAQFDRAADFLAAHGIDLRVFVLVGAPYVEPEASVEWTVRTAGYAARRGATRIALI
                     PVRGGNGEMERLAALGRFVPPGLSQLESALDRCLGLAPVLVAADLWDIDRLPACAECR
                     PQRIERLRRLNLTGGPEAPVVCRACGAP"
BASE COUNT          686 a         1487 c         1426 g          564 t
ORIGIN      
        1 agcgacgtcg atggcgacgg cgaccaggat ctgctcgtcg ccgactccgc gggtgtccat
       61 ctcctgcgca acgacggggg caacgccaat ctggccatgc aggtgcagct catggcgctc
      121 cgcaccggaa gcggcaagaa caacaacttc ggcatcggcg cgacgatcga ggtgcgcgcg
      181 ggggagctgt tccagacgcg ggtcgtgacc ggacggctca cgcccttcgg tctcgggccg
      241 cacctcaagg ccgacgtgct gcgcgtgcag tggaccaacg gtgtgcccga gacggtgtat
      301 ttcccgggca ccgaccagga tgtcctcgag ctcgagcagc tcaagggatc gtgcgcgttc
      361 ctctatacat gggatgggaa gcggttccgc ttcgtgaccg acgtgatgtg gcgcagcgca
      421 ttgggaatgc cgctcggcat catgggcggc ggcagggggg ccgcgtacgc ccccgcggga
      481 gcatcgcagg aatacctccg catccccggc gacgccctcc agttgcgcca gggccgctac
      541 gtcctccagt tcacggagga attgtgggag acggcgtacc tggatgagat cagactcctc
      601 gccgtggatc accccgacag cgtggacgtg tacgtcgacg agcgcttcgt ccctccgggc
      661 ccggtccagc tgcggctgtt ccaggcggtg catcgccgac cgccccgctc ggccgtcgac
      721 gaccgcggcg aggacgtcct gccggcgttg cgcgagcggg atgacgtgta cgtctccaac
      781 ctcacgccca gccgctacca ggggctggtc gaaccgcacg atctcatcct cgatttggac
      841 gatgacgccg gtcgccccgg caccttcctc ttcctgcgcg gctggatcta cccgaccgac
      901 gcgagcatca acgtcgcgct ctcacagcaa acccgcctga gcacgcagct gccggcgctc
      961 gatgtgcgcg acgggcacgg gcgatggacg accgcgctcc cgaacctcgg cttcccgtcg
     1021 gggaaggaca agacgatcgt agtcgacctc gccggaatct tcccgacgaa agaccatcac
     1081 gtgcgcattc gcacgaacat gcagatctac tgggaccagg cgttcgtggc gcgcgatgcc
     1141 ccggccagtc cgagccgcat cacgccgttg ccggccgagt cggccgacct gcatttccgg
     1201 ggcttctcac gaacctatcg gcgcggcggc cgctacggcc cgcactggtt cgcctacgac
     1261 gacgtcagca cggactcccc gtggcgtccc attgccgggg cagtgacccg attcggcacg
     1321 gtgcttccgt tgctggggag cgcggacgac atgtacgcca tcatggcgcc gggcgacgag
     1381 gtcaccgtcc agttcgacgc ccactcggcg cgcatgccgc ccccgggatg gcggcgcacg
     1441 ttcctgctct acaccgacgg ctggatcaaa gacgcggacc tgaacaccgc gttcggcaat
     1501 accgtggagc cgctcccgtt ccatgccatc cggaattatc cgtacgcgcc gggcgaggcc
     1561 tacccgaccg acccggcgca ccagcgctac cggcgggagt acaactcgcg ggtcgtcaca
     1621 ccgcgctgag cggctcctcc ccgcggaatg ccggtgccat gccgcgcaac gccggcagca
     1681 cgctcaccag ctcgtcacgg ctcaacccca gcaaggcgtg tcggtccacc agccggtcga
     1741 cgtcgacggg atagaggtga tgcgccgccg ggtcggcgaa tccaccgata ttgcggggct
     1801 caatcgccgc ggccgcccac gccgcaaacg cgcggcgctg gccgagcgtc ccgcgacgcc
     1861 ctcgacctcg cgtaatgcgc cgcagtcgcc ggtgactctc ccgcggcagc ggctccagta
     1921 gcgcgtcgcc gatgccgaga aacccgctcc acgccgccgt ctccgagtcg cgatggacca
     1981 gccgctgatg ggcctccgca aacgacacgg ccgtgaagta gatcatcgca tgagccgcga
     2041 acaggtcgaa gtgcgccatc gcctcgtagg cgcccgcgac gagccgatcg atctggttcg
     2101 cctccgcacg caagagctct gcatttcggc ccaggtcgtc cgcattcggt acacccgagc
     2161 cgcgcgtgcc acgctcgaag cagagcgcca ggcgctcgat cgcgcgcaga ctccacgcga
     2221 tgcccgtcga gaacagcggg tcgacgacgg cgtacgcgtg cggcaggcgc gcccaccgct
     2281 cccccgcggc gcgcgtgagg cggtgttgga tgcgggatcg aaatacgagg ggcatgagcg
     2341 gtgtcgcctc accgaacgcg gcaccgatgg tgggatagcg gtcgagcagg cggcgccaca
     2401 ggacctcggg gtccggcgtc ggcgacgcgc tcaccgccgc caggccacgg ggcgacagcg
     2461 cgaagcccgc gctcgtcacc ccgtgatcga accgcagggc gtacatccat cccccgtcga
     2521 tgaggtgatg cacggcggcc caatcatccg gataggggcc cgccggcagc tcggggacga
     2581 cgtcggccat cggcgcaacg cccgagaagt ggctgaagac cagcgcggag cgtgtcttcg
     2641 tgggccgcaa gccggaggga atcggcagct gccgcgccag gaagccaccc ggcccggagg
     2701 catcgatcac gaactggacc cggagatcga ggccggcccc agcgtgctgc ccggtgagcc
     2761 ggaccccgtt cgcgtcgatg gcgacgcccg tcaactcgac ccggtcacga aactcgaccc
     2821 ccgccgcgac cgcttcgcgc acgaagtgat gatccacgtc cgcgcgcagc cagtgcatgt
     2881 cggccacggc atcggtcggg ctcgcggcga ccaggagccg ttccgacgcg aatccccggt
     2941 cggcgaacgg ctcgccggga tggtgccggt agaaggtgaa gccgcgcttc aggccgcgac
     3001 gcaggtgagg gaaatgctcc agccagcgcc catgcgccgc caggcggtag cagtccgtca
     3061 gaccgtatcg gcgcgcgagc cgctccatcg acagattggc gagcggcgtg gtcgattcac
     3121 cgatggcgaa ccgcggatgc gcgccgcgct cgagcagcac gacgtcgtgg cccagcacgg
     3181 cgagcacgcg ggcgagcaga gatcccgcga acccgctgcc gacgaccgcg attgtgcacc
     3241 gctgcgtcat ggcgctccgc acgcgcggca gacgaccggc gcctcgggcc cgcccgtcag
     3301 attcagccgg cgcagacgct cgatgcgctg cggccggcat tcggcgcacg ccggcaggcg
     3361 gtcgatatcc cagaggtcgg ccgcgacgag gaccggcgcc agacccaggc accggtcgag
     3421 cgccgattcc agctgcgaca gccccggcgg aacaaagcga cccagcgccg cgagtcgctc
     3481 catttctccg ttgccgccgc gcaccgggat cagcgcgatt ctcgtcgctc cacgccgcgc
     3541 cgcatacccc gccgtgcgca ccgtccattc caccgatgcc tcgggctcga catagggcgc
     3601 gcccacgagc acgaagaccc ggaggtcgat gccgtgcgcg gcgagaaagt cggcggcgcg
     3661 gtcgaattgc gccgggccca ggcgcttgtt caggtggcgg gccgccaccg gatggatggt
     3721 ttccaggccg accgcgactt cgagtcggcc cgggatgagg cgcgcaaact ccagcgtcag
     3781 cggtccaatc gtgctggcgt gcgactcgac cgtcacggcg gcaaacggat gggccagctg
     3841 cgcgagcgcc ggcacgtcca cggcggggac cgcgcgccgg tcgaagaagt tgctggcatt
     3901 atagagcttg agccggtcgg gaacggggcg ctcgagcgcg agcagcacgc gcgcgagctg
     3961 cgccggcaat gctcccggcg gcgtcggccc gtcgacggtc cagcgccaca ggtcgcagaa
     4021 tgagcaggtg aaggggcact ccgccccggc gaggaatacc gtcagcgagc gctcgagggc
     4081 gccacttggg ctgcgttcat gctccaggat cgaagtgtgt gcgatccagg ggtcgacctg
     4141 gcgtctcggg gggcggaggc tgc
//
LOCUS       DBNGZW010000357         2523 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_645039, whole genome shotgun sequence.
ACCESSION   DBNGZW010000357 DBNGZW010000000
VERSION     DBNGZW010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2523)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2523)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2523
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_645039"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="AC16JC_08430"
     CDS             <1..1080
                     /locus_tag="AC16JC_08430"
                     /inference="COORDINATES: protein motif:HMM:NF019640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5164760.1"
                     /translation="DAVRGLAPKITARAAEIEAARRLPADLLRDLTAAGCFRMLVPRS
                     HGGNGFDLAGAMSVCEELSRADASVAWTTMNLAGGWINLGTLPRATFDALYAEGPDAI
                     IGGVFAPSGTAVPADGGYRVSGRWAFASGCQDCQWLFANCMENQAGGPRLRTILFSPE
                     QMKIEDTWSVSGLCGTGSHHLNAEGVFVPAERTCLPFADPACLDEPLLRMPMPPIFAF
                     GVASVAVGIAQGALEDILALSARKVPLLAHSSLATNPLFQNQLGAADAKLRAARGLLY
                     ADAAAAWGTAVAGAEFTPEHRARMRSAITAYHAGGGSSIYSASSLQRRFRDVHAITQH
                     FLVKLDTLTTAGAVLAGAEADLSVF"
     gene            complement(1109..2137)
                     /locus_tag="AC16JC_08435"
     CDS             complement(1109..2137)
                     /locus_tag="AC16JC_08435"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR5164761.1"
                     /translation="MLTQRVAASRALGVVAAAVALGTALVYGQVRDAGPIEQVTLRSG
                     SYADFRQVFVREAPADVSVPATLRFPDQPRDRYPAVVVVHTISGFREANEGWLAAELR
                     RSGFATLTYDSFSVRGLSQSAVVGSRAGPPLASGVADAFAALRLLAGHPRIAADRIGI
                     VGFSFGGEVAHLTALEALRAAMGAGPSRFAAHVATYPGGTYGARAEAGAYTGAPILML
                     LGEKDDNLPVAKAEGYLAYAIAAGHPAPIATVVYPGAFHAWTAPTLGAPRFYPQYAAS
                     SRKCPVILLGRGAPMMLVDGRERPFDPDALAACQAAGSGYSMGFDAATRTKSSTDTTA
                     FLQRHLRP"
     gene            complement(2174..>2523)
                     /locus_tag="AC16JC_08440"
     CDS             complement(2174..>2523)
                     /locus_tag="AC16JC_08440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164762.1"
                     /translation="PGLSALAGVAILLGFVPLCVLLAVTIIGIPLIPVAGMLLVALFL
                     FGFTVSAAWLGERLPFLQEKTPVKTVAVGGAVFVLVGLIPWIGTAALLLAAAFSAGAT
                     LLSRFRRTPVVAV"
BASE COUNT          381 a          898 c          906 g          338 t
ORIGIN      
        1 gatgccgtcc gcggcctggc tccgaagatc accgcccgtg ccgccgagat cgaggccgcg
       61 cggcggctgc ccgccgacct cctccgggat ctgaccgcgg ccggctgctt ccgcatgctg
      121 gtgcctcgca gccacggtgg caatggcttc gacctggccg gcgcgatgag cgtgtgcgag
      181 gagctgagcc gggcggatgc gtccgtggcc tggacgacga tgaacctggc gggcggctgg
      241 atcaatctgg gcacgcttcc ccgggcgacg ttcgatgccc tctacgccga gggtcccgac
      301 gccatcatcg gcggtgtctt cgctccttcg ggaacggccg tgcccgcgga cggcggctac
      361 cgggtgagcg gccggtgggc gttcgccagc ggttgccagg actgccagtg gctgttcgcc
      421 aactgcatgg agaaccaggc cggcggcccc cgcctgcgca ccatcctgtt ctcgccagaa
      481 cagatgaaga tcgaggacac ctggtcggtc tcggggttgt gcggcaccgg cagccatcat
      541 ttgaatgccg agggtgtgtt cgtgccggcc gagcggacgt gcctgccctt cgccgatccg
      601 gcgtgcctgg acgagccgct gctgcggatg cccatgccgc cgatcttcgc cttcggggtc
      661 gccagcgtcg cggtcggcat cgcccagggg gcgctggagg acatcctggc cctctccgcc
      721 cgcaaggtgc ccttgctcgc gcactcctcg ctggccacga acccgctgtt ccagaaccag
      781 ctcggcgccg ccgacgcgaa gctccgcgcg gctcgcggac tgctgtacgc ggacgccgcg
      841 gccgcgtggg ggacggcggt ggccggtgca gagttcacgc ccgagcaccg ggcccggatg
      901 cgctcggcga tcacggccta ccacgcgggg ggcgggagct cgatctactc cgccagctcc
      961 ctccagcgcc gcttccgcga cgtccacgcg atcacccagc atttcctggt caagctcgac
     1021 accctcacca ccgcgggagc cgtgctcgcg ggcgcggagg ccgacctgag cgtgttctga
     1081 aggtagagac gcggcgccga tccccgtctc acgggcgcag atggcgctgg aggaaagccg
     1141 tcgtgtcggt ggaggatttg gtgcgggtgg ccgcgtcgaa ccccatcgag tagcccgacc
     1201 ccgcggcttg gcacgcggcg agcgcatcgg ggtcgaacgg ccgctcgcgc ccgtccacga
     1261 gcatcatggg cgccccgcgc cccaacagga tcacggggca cttccgcgag ctagcggcgt
     1321 actgggggta gaaccgcggc gcgcccaggg tgggtgcggt ccaggcgtgg aacgcgcccg
     1381 gatagaccac cgtcgcgatc ggcgccggat gacccgcggc gatcgcgtat gcgagatagc
     1441 cctcggcctt ggccaccggc aggttgtcgt ccttctcccc gagcagcatc aggatcggag
     1501 cgccggtata cgcgcccgcc tcggcccgcg cgccgtaggt gccgcccgga taggtggcga
     1561 cgtgagccgc gaaccgcgac ggccccgccc ccatcgcggc acggagcgcc tcgagtgcgg
     1621 tgagatgcgc cacctcgccg ccgaacgaga agccgacgat gccgatgcgg tcggcggcga
     1681 tccgcgggtg tccggccagc aggcggagcg cagcgaacgc atcggccacc ccggacgcca
     1741 gtggcgggcc ggcgcgggag ccgacgaccg cggactggct caggcccctc acgctgaaac
     1801 tgtcgtaggt cagcgtcgcg aacccgcttc ggcgcagctc cgccgccagc cagccctcgt
     1861 tggcctcccg gaagccgctg atcgtgtgga ctaccaccac cgccggatac cgatcccgcg
     1921 gctgatcggg aaagcggagc gtcgcgggga ccgatacatc cgccggcgcc tcccgcacga
     1981 agacctggcg gaagtcggca taggagcccg agcggagcgt cacctgctcg atcgggcccg
     2041 catcgcggac ctgaccgtat acgagcgccg tcccgagcgc gaccgcggcg gccaccaccc
     2101 ccagcgcccg cgacgccgcc actcgttgtg ttagcacgag gcgtgaattc cttcgtcgac
     2161 ggggtggttc ggcctagacg gcaacaaccg gtgtgcgcct gaagcgcgag aggagcgtcg
     2221 cgccggctga gaaggcggcc gcgaggagca gcgcggcggt gccgatccag ggtatcaggc
     2281 cgacgaggac gaagacggcg cctcccacgg cgaccgtctt gacgggtgtt ttctcctgaa
     2341 ggaacggcag cctctcgccg agccatgcgg cggagacggt gaacccgaac aggaagaggg
     2401 cgacgagaag catccccgcc accgggatca gcggaatgcc gatgatcgtc acggcgagca
     2461 ggacgcagag cggcacgaat cccaggagga tcgcgacgcc ggcgagcgcc gagagcccgg
     2521 gtc
//
LOCUS       DBNGZW010000358         4822 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1533488, whole genome shotgun sequence.
ACCESSION   DBNGZW010000358 DBNGZW010000000
VERSION     DBNGZW010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4822)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4822)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4822
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1533488"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="AC16JC_08445"
     CDS             complement(<1..907)
                     /locus_tag="AC16JC_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR5164763.1"
                     /translation="MKPLATIFAALAAAPLLGSAIAAGAMAADEQTKKRHRMHDWRSL
                     DGLPDDPGLPALAAIRASSVAKTIPALGLDGRSTEVTLCGYTAGRRATLQVRTGDRRI
                     AVKAYSESPELEAQLYAALGGGYGGLHVPRLLAWDPQLRVVALEWLDGPSLSSRIEDG
                     QGRRAGELAAAWVRRAAELPVRFGSHHAVEGLLAKAYKWADGLGATDRALGTAAAAVA
                     RRLVATRPVESGSQLVHGSLYDRHILDLGDGPGLIDWDCFGQGAPELDAAVFLAGVWR
                     RGLRPKWEDASAQTTEAFLACAKGLL"
     gene            1265..3187
                     /locus_tag="AC16JC_08450"
     CDS             1265..3187
                     /locus_tag="AC16JC_08450"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR5164764.1"
                     /translation="MTNAHGSDGPMPALRRLLMAAALIAVACTDSQRTGPTSDITGLV
                     ITPDSVATLAGDTVQFHALGWTADGSTQAVAVRWSASGGTIDGDGRYIAGTVAGEFRA
                     TATLDAPHLAAGASIHNRGTLLRVLLLPESATVAPGGRVWFSASAQAADGESAPVRVT
                     YAATGGSIDSSGAYLAGATAGDFQVVATARGGTLGDTSRVTVAAGAPTLTGVVLAPPS
                     VTLSDGSTQRFSAYGTLSNGDSVAVGVIFSATGGTITPDGLYTAGRTAGNFRVIASEQ
                     GGMLADSADVTIVAGAPVLTQVVLVPPSVPLNAGGSQQFAAYGTLSNGDSVAVSASFS
                     ATGGTITASGLYTAGNTAGDFRVVAAASGAMLADTASVHITSPGSAVVLVGAGDIADC
                     GSDGDEATAALLDGIPGTVFTAGDNAYSDGSPTDYSGCYEPSWGRHRTRTRPAPGNHD
                     YNTSGAAGYFAYFGDAAGPPGRGYYSYDLGDWHIVSLNSEVDMSAGSTQETWLRADLA
                     ANSKQCTLAYWHKPRFSSGTKHGSDSETQPLWQALYDYGAEIAIAGHDHDYERFAQQT
                     PSGTADPARGIREFVVGTGGSSHSGVGTPLPTSEASDGATFGVLKLTLGAGTYAWQFV
                     PVAGGSFTDSGSGTCH"
     gene            complement(3156..4295)
                     /locus_tag="AC16JC_08455"
     CDS             complement(3156..4295)
                     /locus_tag="AC16JC_08455"
                     /inference="COORDINATES: protein motif:HMM:NF014396.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016158 - inositol hexakisphosphate
                     3-phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytase"
                     /protein_id="HIR5164765.1"
                     /translation="MSKLKKTLIACWLGGLWAVDGAASQASKPVLAGIAPTARPEARA
                     QTRPVPHTGDAADDPAIWIHPRDPALSLVLGTDKKGGLHAYDVDGSELQVVGQDTRPN
                     NVDVLYGFPLAGRPVDLAVATTQAPEDRRGLRVWAIDAVTRRLSDVTDGGAIAVFGGD
                     VPYGVCGYHSARTGRFYVFVTGRDGRVEQYELRPGGRGAIGATRVGAFSVASTAEGCV
                     ADEELGVFYLAEESAGIWRFAAEPDGREVGSAGRLVARVGEHGLAADVEGLALYYAAE
                     GRGYLIASSQGNDSYKVYERGGDNRYVLTIDPVGGRIDDVDHTDGIAVASCPLSAQLS
                     RGVFVVQDGHNSGGNQNFKLYGWEDVAGTSLVVDTVSGRCRSRSR"
     gene            complement(4295..>4822)
                     /locus_tag="AC16JC_08460"
     CDS             complement(4295..>4822)
                     /locus_tag="AC16JC_08460"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR5164766.1"
                     /translation="DATIPLHHTRDDQLADVQRAGTLVQWACPESRGTVRQIVSALEG
                     GLAEVSPAPIHRDLKPDHVFLSGGRVRFIDCDSVALGDPARDPAHLFAHILGGVGLEA
                     MPFAHRAGAATAFVLEYFAHVPSHWRRGFRLHCAGALLEVAGGIFKRQEPHWREKVTA
                     AVAEAAACLRQERSE"
BASE COUNT          732 a         1736 c         1664 g          690 t
ORIGIN      
        1 tcaggagccc cttcgcgcac gcgaggaacg cttcggtcgt ctgcgccgac gcgtcctccc
       61 acttcggccg caaccccctg cgccagaccc ccgcgaggaa cacggcggcg tcgagctcgg
      121 gcgctccctg gccgaaacag tcccagtcga tcaggccggg gccgtctccc aggtcgagaa
      181 tgtgtcggtc gtacaacgag ccgtgcacga gttgcgagcc ggactcgacg ggtcgggtgg
      241 ccaccagccg acgggcgacc gccgccgcgg cggtccccag cgcccgatcg gttgcaccca
      301 gaccatccgc ccacttgtac gctttcgcga gcagcccctc gaccgcgtgg tgagagccga
      361 accggaccgg aagctcagcc gcgcgccgca cccacgcggc ggccagctcg cccgcgcgtc
      421 gtccctgccc gtcctcgatc cggctcgaca gggagggacc gtccagccat tcgagggcga
      481 ccacccggag ctggggatcc cacgcgagga gccgaggcac gtgcagtccg ccgtagccgc
      541 cgcccaaggc cgcgtacaat tgggcttcca actccggcga ctccgagtac gctttcactg
      601 caatgcggcg gtcgccggtg cgcacttgca gtgtggcgcg ccggccggcg gtatagccgc
      661 acagcgtgac ctcggtggag cgaccgtcaa ggccgagcgc ggggatcgtc ttggcgacgc
      721 tcgaggcgcg gattgccgcg agcgccggca accccgggtc gtcggggagt ccatccaacg
      781 atcgccagtc gtgcatgcgg tgccgcttct tcgtctgctc atcggcggcc atggcgcctg
      841 cggctatcgc acttcccagt agcggcgcgg cggccagcgc ggcgaagatt gtagccaacg
      901 gcttcatgcc agccccccac ggggtcggtg atgccccgag gggtggcatg cttcggaccg
      961 tcactcgtag ctggcacttg ccccgttcag gagcacgcag caccacgatt tcgggccgag
     1021 tctgtggcag cggcgcgcca ctcgtcgcac tgccctgcga cttcctgcgt gcgacctcac
     1081 acctcaccgg aggtacggcg atggacgtgg cgcggtgttg cccgacggcg agagcgccgg
     1141 cccggcacat tcgtctgcgg cccgggagtt gccaagcacc caacgcggcg aggcggtcga
     1201 gtgacggtga cgcacgagtg cgggttacca cggagtggcc aatgacggtc gcgatgtgcc
     1261 gctgatgacc aacgcgcacg ggtcggacgg cccgatgcct gcgctccgcc gcttgctgat
     1321 ggcggccgcg ctgatcgcag ttgcctgcac ggactcccag agaaccggtc cgaccagcga
     1381 catcaccgga ctcgtgatta cgcccgattc cgtcgcgacg ctggctggtg acacggtgca
     1441 gttccacgcc ctgggctgga cggcggacgg ctccacccag gcggttgcgg tgaggtggag
     1501 cgcctcgggc ggcacgatcg acggcgacgg gcgctacatc gcgggcacgg tcgccggcga
     1561 attcagggcg acggccacac tggacgctcc acacctggcg gccggcgcca gcatccacaa
     1621 ccggggcacg ctcctccgcg tgttgctgtt gcccgaatcg gcgacggtcg cgccgggcgg
     1681 tcgggtgtgg ttctccgcct cagcgcaggc ggccgatggc gagagtgccc cggtccgcgt
     1741 gacctatgcc gccacgggcg gctcgatcga ctcgagcggc gcctacctcg ccggcgcgac
     1801 ggcgggggac ttccaggtcg tcgccacggc gcgaggcggg acgctcggcg acacctcgcg
     1861 cgtcaccgtc gccgcgggag cgccgacgct gacgggggtg gtgctggcgc cgccttcggt
     1921 gacgctcagt gacgggagca cacagcggtt ctccgcctac ggcacgctga gcaacgggga
     1981 ctcggtcgcc gtcggtgtga tcttctccgc cacggggggc acgatcaccc ctgacggact
     2041 gtacacggcg ggcaggacgg cggggaattt ccgcgtgatc gcctccgagc aaggggggat
     2101 gctggccgac tccgcagacg tgacgatcgt cgcaggagcg ccggtgttga cgcaggtggt
     2161 gctggtcccc ccctcggtgc cgctcaacgc cggcggctcc cagcagttcg cggcctacgg
     2221 cacgttgagc aacggggact cggtcgccgt gagcgcgagc ttctcggcca cgggcgggac
     2281 gatcaccgcc agcgggctgt acacggcggg caacaccgca ggggacttcc gggtggtcgc
     2341 cgcggcgtcg ggtgcgatgc tggccgacac cgcgagcgtg cacatcacgt cgcccggaag
     2401 cgcggtggtg ctcgtgggcg ccggggacat cgccgactgc ggctcagacg gcgacgaagc
     2461 gacggccgcc ctgctggacg gcatcccggg caccgtgttc acggccggcg acaacgccta
     2521 ctccgacggg agcccgaccg attacagcgg gtgctacgaa ccgagctggg ggcgccacag
     2581 gacgcgaacg cgcccggcgc cgggcaatca tgactacaac acctccggcg ccgcgggcta
     2641 cttcgcctac ttcggcgacg ccgcggggcc gccgggccgg ggctactaca gctacgatct
     2701 gggcgactgg cacatcgttt cgctgaacag cgaggtggac atgagcgccg gctcgacgca
     2761 ggagacgtgg ctgcgtgccg acctggcggc caactccaag cagtgcacgc tggcctattg
     2821 gcacaaaccg cggttcagca gcggcacgaa gcacggcagc gacagcgaga cgcagcccct
     2881 gtggcaggcg ctgtacgact atggggccga aatcgccatc gcgggacacg accacgacta
     2941 cgagcgcttc gcccagcaga cgccatcggg gaccgccgat ccggcgcgag gaatccgtga
     3001 gttcgtggtg gggaccgggg gaagcagcca cagcggcgtg ggcacgccgc tgccgactag
     3061 cgaggcgtcc gacggcgcga cgttcggggt gctgaagctc acgctgggcg cgggcacgta
     3121 cgcctggcag ttcgtccccg tcgccggagg atcgttcacc gactccggga gcggcacctg
     3181 ccactgacgg tgtcgaccac caggctcgtt cccgcgacgt cttcccagcc gtagagcttg
     3241 aagttctggt tcccgcccga gttgtggcca tcctgcacga cgaacacgcc gcgggatagc
     3301 tgggctgaca gcgggcagct ggccacggcg atgccgtcgg tgtgatccac atcgtcgatc
     3361 cggccgccca ctggatcgat cgtcagcacg taacggttgt ccccgccccg ctcgtacacc
     3421 ttgtacgagt cgttgccctg actcgaggcg atcagatagc cgcgcccctc cgccgcgtag
     3481 tacaacgcca gcccttctac gtccgcggcc aggccatgct cacccacgcg cgccaccagg
     3541 cgccctgcgc tcccgacctc gcgtccgtcc ggctccgcgg cgaacctcca gatccccgcg
     3601 ctttcttcgg ccagatagaa gaccccgagc tcctcgtccg ccacgcatcc ctcagccgtg
     3661 gatgccaccg agaaggcgcc gacccgggtc gcaccgatcg caccgcgacc cccggggcga
     3721 agctcgtact gttccacgcg accgtcccgc ccggtgacga agacgtagaa gcgccccgtg
     3781 cgagcgctat gatacccgca cacgccgtag gggacgtccc caccgaacac cgcgatcgcg
     3841 ccgccatcgg tcacgtcgct gaggcgccgc gtcaccgcat cgatcgccca caccctcagg
     3901 ccgcgacggt cctcgggcgc ctgcgtcgtg gcgacggcca ggtccaccgg ccgcccggcc
     3961 agcgggaacc cgtagagcac atccacattg ttgggccgcg tgtcctggcc gacgacctga
     4021 agctcggagc cgtccacgtc gtaggcatgc agcccgcctt tcttgtccgt gccgagaacg
     4081 aggctcaggg caggatcccg gggatggatc cagatcgccg gatcgtcggc tgcgtcgccg
     4141 gtgtgcggga cgggccgggt ctgtgcccgc gcctcgggcc gcgcggtcgg cgcgattccc
     4201 gccaggaccg gcttactcgc ctgggaggcc gctccgtcga cggcccacag acccccgagc
     4261 caacacgcaa tcagggtctt cttcagtttg ctcatcactc ggacctttcc tgtcgcaggc
     4321 acgccgcggc ttccgccacc gccgccgtca ctttttcgcg ccagtgcggc tcctgccgtt
     4381 tgaagattcc gcccgccact tcgaggaggg cgccggcgca gtgcagccgg aaccctcggc
     4441 gccagtggct ggggacatgc gcgaagtact cgagcacgaa ggccgtagcg gctccggcgc
     4501 ggtgggcgaa cggcatggct tccagaccga ccccgccaag gatgtgggca aacaggtgcg
     4561 cggggtcgcg ggcgggatcg cccagggcga ccgaatcgca atcgataaac cggacgcggc
     4621 cgccggacag gaacacgtgg tcgggcttca ggtcccgatg gatcggtgcg ggactgacct
     4681 ccgccagccc accctcgagt gccgagacga tctgtcggac ggtccctcgg ctctcgggac
     4741 aggcccattg caccagcgtt cctgcccgtt gcacgtcggc aagctgatcg tcgcgcgtgt
     4801 ggtgcagcgg gatcgtcgcg tc
//
LOCUS       DBNGZW010000359         2114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_92959, whole genome shotgun sequence.
ACCESSION   DBNGZW010000359 DBNGZW010000000
VERSION     DBNGZW010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_92959"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="AC16JC_08465"
     CDS             complement(<1..678)
                     /locus_tag="AC16JC_08465"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002609730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR5164767.1"
                     /translation="MLTDDRGRAIDALFADYSGSAVPGASVIVIHGGRVIFRSAYGMA
                     DLERRVPATTHTDYRLASVTKQFTAMAIMLLAGDGRLRYDQPVREILPELPPAAAPVT
                     IRHLLNHTSGLLDYEDLIPDSQALQVHDRDVLTLLAAHDTVYFPAGTRYRYSNSGYCL
                     LALVVERVSGMPFARFLQERIFARVGMSGTVAHQDGVDTVPQRAYGYTPDSGRFRPSD
                     QSVTSATL"
     gene            complement(738..1712)
                     /locus_tag="AC16JC_08470"
     CDS             complement(738..1712)
                     /locus_tag="AC16JC_08470"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5164768.1"
                     /translation="MLPLIWSLALSRGTPAPAPPPLRPGTVRSERFFSPALGVWKRYL
                     VYLPPSYDRQPAHRYPVAYYLHGLGGNESDWLSRADIAGVMDSLIANGAPELILVLPD
                     GDNGWYAAWSEPGDSAACAADSTLAEAAETYCVARQRYDDYIAVDLVAHIDSTYRTIA
                     DRAHRALAGLSMGGYGAVTLALRFPTAFAAAASHSGVLSPFYAGPHPFSEPAHYLTSR
                     DSLPAIWGGLWRWMAPVFGGDTTRWWAGDPAHLVRRLVAMHRYVPALYFDVGTSDSHA
                     DENRALHAELTALGVPHEYAEWPGKHNWPYWRAHVGESLAWLARRLTR"
     gene            complement(1718..>2114)
                     /locus_tag="AC16JC_08475"
     CDS             complement(1718..>2114)
                     /locus_tag="AC16JC_08475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164769.1"
                     /translation="GMMVVESWARLWHDGSACWPEPQFSKPLRIEGTMLLDLQVDLPV
                     VLPALLRPWTDVPQPPRQPTRLLVARSKRGRVSVARTNATVGTLLQLADGSKSIEELA
                     REAGLRPAELEATLQQLVDIGAVRFSTGS"
BASE COUNT          354 a          705 c          762 g          293 t
ORIGIN      
        1 cagtgtcgca ctggtgacgc tctgatcgct gggccggaaa cggccggaat ccggggtgta
       61 gccgtacgcc cgctgcggca cggtgtccac gccgtcctgg tgggccacgg tccccgacat
      121 cccgacccgg gcgaagatgc gctcctgcag aaagcgcgcg aacggcatgc cggacacacg
      181 ctccacgacc agcgccagca gacaatagcc ggagttgctg tagcgatagc gcgtgcccgc
      241 cgggaagtac acggtgtcgt gcgctgcgag cagggtcagg acatcgcggt cgtgcacctg
      301 cagggcttgc gaatcgggaa tgaggtcctc gtaatcgagc aatcccgacg tgtgattcag
      361 cagatggcgg atggtgaccg gggccgcggc gggaggcagc tcgggcagga tctcgcgcac
      421 cggctggtcg tagcgcagcc gcccgtcccc ggccagcagc atgatcgcca tcgcggtgaa
      481 ttgcttggtg accgacgcca gacgatagtc ggtgtgggtc gtggccggga cccggcgctc
      541 gaggtcggcc atgccgtagg cgctgcgaaa gatcacccgg ccgccgtgga tcacgatcac
      601 gctcgcgccg ggaaccgcgg atccggagta atcggcgaac agcgcgtcga tggcccggcc
      661 gcggtcatcg gtcagcatgc cgccgctcgc acaagccgtg acggcgcaca ttatcaggag
      721 ggaccgccgc acgtccctca ccgggtgagc cgccgggcca gccaggcgag gctttcaccg
      781 acgtgggcgc gccagtacgg ccagttgtgt ttgccgggcc attccgcgta ttcatggggc
      841 actcccaggg ccgtcagctc ggcgtgtaat gcgcgattct cgtcggcgtg gctgtccgag
      901 gtcccgacgt cgaagtacag cgccgggacg taccggtgca tggcgaccag tcgccggacc
      961 aggtgcgccg gatctccggc ccaccagcgc gtcgtgtcgc cgccgaacac cggggccatc
     1021 cagcgccaca ggcctcccca gattgcgggg agcgagtcgc gcgacgtgag atagtgcgcg
     1081 ggctccgaga agggatgcgg ccccgcgtag aagggcgaca acacacccga gtggctggcc
     1141 gcggctgcga acgccgtggg gaaccgcaga gccagcgtca ccgcgccata tccgcccatg
     1201 ctgagccccg cgagggcgcg gtgggcgcga tcggcgatgg tgcgataggt cgagtcgatg
     1261 tgcgccacga gatcgaccgc gatgtagtcg tcatagcgct ggcgcgccac gcaatacgtt
     1321 tcggccgctt ccgccagcgt ggaatcggcc gcgcacgcgg ctgagtcacc gggctcgctc
     1381 caggcggcat accatccgtt gtcgccgtcg gggagcacca ggatgagctc gggagcgcca
     1441 ttggcgatca gggagtccat cacgcccgcg atgtcggccc gggagagcca atcggactcg
     1501 ttgccaccga gcccgtgcag gtaataggcg acgggatagc ggtgcgccgg ctgtcggtcg
     1561 taggacggag gcaggtagac gaggtaccgt ttccacaccc ccagagccgg cgaaaagaac
     1621 cgttccgaac gtacggtgcc cggccggagc ggtggaggtg ccggagcagg cgtgccgcgg
     1681 gacagtgcga gggaccagat caggggcagc atacgtgtca gctgccggtc gaaaaccgca
     1741 cggcgccgat gtcgacgagc tgttgcagcg tcgcctcgag ctccgcgggc cgcaaccccg
     1801 cttcccgagc cagctcctcg atggatttgc tgccgtcggc gagctggagc agcgtcccga
     1861 cggtcgcatt ggtccgcgcc accgacaccc ggccgcgttt cgagcgcgcg acgagcagcc
     1921 gcgtgggctg ccgcggcggc tgcggcacgt cggtccacgg acgcagcagc gccggcagca
     1981 cgacggggag gtccacctgc aggtcgagca gcatcgtgcc ctcgatccgg aggggcttcg
     2041 agaactgcgg ctccggccag caggccgagc cgtcgtgcca caaccgggcc caggactcga
     2101 cgaccatcat gccc
//
LOCUS       DBNGZW010000360         2713 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1668202, whole genome shotgun sequence.
ACCESSION   DBNGZW010000360 DBNGZW010000000
VERSION     DBNGZW010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1668202"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..658
                     /locus_tag="AC16JC_08480"
     CDS             <1..658
                     /locus_tag="AC16JC_08480"
                     /inference="COORDINATES: protein motif:HMM:NF016764.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HIR5164770.1"
                     /translation="VLFSPTEVFERVRERPKFLVPALGVAAIVTLLSYLMMPFTQAAM
                     ASRMAEIAQQNPAAAAQAAKFQSIGIVFAPITVFIVLLVVAGFLWLLVMLLAGGDAKY
                     KTLLSVATYTALPSILLQAAGLLVLKVKGVEAVSSMQDLRPPLGLNLLAPNATGFMGA
                     LLAGINPFAIWSMVLTAIGIAVTHKTSKGSAYTVAIVAMLLGLLIGAAFAGLGNRGGS
                     "
     gene            complement(662..>2713)
                     /gene="ppdK"
                     /locus_tag="AC16JC_08485"
     CDS             complement(662..>2713)
                     /gene="ppdK"
                     /locus_tag="AC16JC_08485"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR5164771.1"
                     /translation="PHAQLWGAIEAVFKSWRTRRAIDYRRVHGIPDDLGTAVNIVAMV
                     YGNLGEDCGTGVAFTRDCRTGERVLNGDFLINAQGEDVVSGARTPDSIAKMKRGKFAK
                     VYRELETIAEKLERHFKDVQDIEFTFEHGHLYMLQTRRAQRTGLAAVRSAVEMVAEGL
                     ISENEAVQRVPPQDLDQIFHPMVDPRASVTLVAKGLPASPGAAIGEAVFDADQAEALA
                     KKGHKVILVRPETSPEDFHGIVAAQAVVTSRGGMTSHAAIVARGMGKTCVVGAKEIEV
                     EPAAGRFRANGRVVKQGQVLTVDGTTGRVILGAAKLVQPRIGEHYARLMEWADARRRL
                     RVRTNADTPADARRAREFGAEGIGLCRTEHMFFEGDRITAMREMIVAPDEAGRRRALA
                     KLLPMQRADFEGIFEAMVGYPVTIRLLDPPLHEFLPHGREEQSALAKAMHLPLAQIKA
                     LVEGLHEANPMLGHRGCRLGITYPKITEMQARAIFEAACRAVARGKKVVVEVMIPLVA
                     AVEELRRQAAIVRRVAQEVFHAQGHAVPYHVGTMIELPRAALTADEIAREAEFFSFGT
                     NDLTQTTFGLSRDDAGRFLPFYVETGILKDDPFQVLDQDGVGRLVELGVKLGRGTRND
                     LKVGICGEHGGDPSSVKFCHHAGLNYVSCSPFRVPIARLAAAQAALEEAGVINRTKAT
                     V"
BASE COUNT          391 a          929 c          938 g          455 t
ORIGIN      
        1 ggtgctgttc tccccaacgg aagtgttcga gcgcgtgcgg gagcgtccga aattcctggt
       61 cccggcgctc ggcgtggcgg cgatcgtgac cctcctgagc tatctgatga tgccgttcac
      121 ccaggcagcc atggcatccc gcatggcgga gatcgcgcag cagaatccgg ccgcggcggc
      181 acaggcggcc aagttccagt cgatcgggat cgtcttcgcg ccgatcacgg tgttcatcgt
      241 gctgctggtc gtggcggggt tcttgtggct actcgtcatg ctgctcgccg ggggggacgc
      301 gaagtacaag acgctgttga gcgtcgcgac ctacacggcg ctgccgtcga tcctgttgca
      361 ggcggcggga ctgctcgtcc ttaaggtgaa gggcgtcgag gcggtgagct cgatgcagga
      421 tctacgcccg cccctgggtc tcaacctgct ggcgcccaac gccacagggt tcatgggcgc
      481 gctcctcgcg gggatcaacc ccttcgcgat ctggagcatg gtgctgacgg cgatcgggat
      541 cgcggtgacg cacaagacat ccaaaggctc ggcgtacacc gtggcgatcg tggcgatgct
      601 gctcggcctc ctgatcgggg ccgcgttcgc ggggctggga aaccgcggcg gcagctgagg
      661 gctagaccgt cgccttggtg cggttgatga cgcccgcctc ctcgagggcc gcctgggccg
      721 cggcgaggcg ggcgatcggg acccggaacg gggagcacga gacgtagttg agcccggcgt
      781 ggtggcagaa cttcaccgac gaagggtcgc cgccgtgctc cccgcagatc cccaccttca
      841 ggtcgttgcg cgtgccgcgg cccagcttca cccccagctc gacgagccgg ccgacgccgt
      901 cctgatccag gacctggaac ggatcgtctt tcaggattcc ggtctccacg tagaacggca
      961 ggaagcgacc ggcgtcgtcg cgggagaggc cgaaggtcgt ctgcgtgagg tcgttggtgc
     1021 cgaacgagaa gaactcggct tcccgggcga tctcgtcggc ggtgagcgcc gcgcgcggca
     1081 gctcgatcat cgtgcccacg tggtacggca cggcgtgccc ctgggcgtga aacacctcct
     1141 gcgcgacgcg acgcacgatc gccgcctggc gccgcagctc ctcgactgcc gcgaccagcg
     1201 ggatcatcac ctcgacgacc actttcttcc cgcgcgcgac cgcgcggcag gccgcctcga
     1261 agatcgcccg ggcctgcatc tccgtgatct tggggtaggt gatgcccagc cggcagccgc
     1321 ggtggccgag catcgggttc gcttcgtgga ggccctccac gagtgccttg atctgcgcca
     1381 ggggcaggtg catggccttg gcgagagcgc tctgctcctc ccggccgtga ggaaggaatt
     1441 cgtgcagcgg agggtcgagc aaccggatcg tgaccggata cccgaccatc gcctcgaaga
     1501 tgccctcgaa gtcggcccgc tgcatcggca gcagcttggc gagcgcccgg cggcggccgg
     1561 cctcgtcggg cgcgacgatc atctcccgca tcgccgtgat gcggtctccc tcgaagaaca
     1621 tgtgctcggt gcggcacagg ccgattccct cggcgccgaa ctcgcgcgcg cgacgggcat
     1681 ccgccggggt atcggcgttg gtgcggaccc gcaggcgccg ccgggcgtcc gcccactcca
     1741 tcaaccgggc atagtgctcg ccgatccggg gctgcacgag cttcgcggcg ccgaggatca
     1801 cacgaccggt cgtgccgtcc accgtcagga cctggccttg cttcacgacc cggccgttgg
     1861 cgcggaaccg tccggcggcc ggctccactt cgatctcctt tgccccgacg acgcaggtct
     1921 tgcccatgcc gcgcgcgacg atggccgcat ggctcgtcat tccgccgcgc gaggtgacga
     1981 ccgcctgggc ggcgacgatg ccgtggaaat cctcgggaga tgtctcgggg cggaccagga
     2041 tcaccttgtg acccttcttc gccagcgctt cggcctggtc cgcgtcgaac acggcctcgc
     2101 cgatcgcagc gccgggcgag gcgggcaacc ccttcgctac gagggtgacg ctggcccggg
     2161 ggtcgaccat ggggtggaag atctggtcga gatcctgagg cggcacccgc tgcaccgcct
     2221 cgttctccga gatcaatccc tcggcgacca tctccacggc gctgcgcacg gccgcgagcc
     2281 cggtgcgctg ggcgcgccgt gtctgcagca tgtagaggtg gccgtgctcg aaggtgaact
     2341 cgatgtcctg cacgtccttg aagtggcgct cgagtttctc cgcgatcgtc tcgagctcgc
     2401 gatacacctt ggcgaacttg ccgcgcttca tcttggcgat cgaatcgggg gtgcgcgccc
     2461 ccgacacgac gtcttccccc tgcgcattga tgaggaagtc gccattgagc acccgctccc
     2521 cggtacggca gtcgcgcgtg aacgcgacgc cggttccgca atcctcgccc aggttgccgt
     2581 agaccatcgc gacgatgttc accgcggtgc ccaggtcgtc ggggatgccg tggacgcgcc
     2641 ggtagtcgat cgcgcgccgg gtgcgccagc tcttgaacac ggcctcgatc gcgccccaca
     2701 gctgggcatg ggg
//
LOCUS       DBNGZW010000361         4385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1046159, whole genome shotgun sequence.
ACCESSION   DBNGZW010000361 DBNGZW010000000
VERSION     DBNGZW010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4385
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1046159"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1228)
                     /locus_tag="AC16JC_08490"
     CDS             complement(<1..1228)
                     /locus_tag="AC16JC_08490"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR5164772.1"
                     /translation="MAQIPVADPRVAFIEAACWHGMLERAEAILAEHPELHSADIHIA
                     AILGDEAAVRRFIARDPASVTAQSPPWGGDPLNYLGLSKYLRLHPERTEDFVRTATAL
                     LDAGAPAHTGFWTTGQYPEFETALYGAAGVAHNEPLTRLLIERGADPNDVDAVYHAPE
                     GRDLGALKVLVETGRVTPENLLLMLIRKLDWHDFEGVRYLLARGANLTGKRSRGSPAL
                     HHALERDNSLQIVELLLDNGADPNEVDEGRSAIALAGRKGRSDVLAALERRGVPLVLR
                     GVERLHAALARADAAAAHAIAQDEPALLREVLADGATLLSEFTLTWNNAGVRLLLELG
                     VPATARYDGYGYFDIAGDSTALHVAAWMLNTELVQLFLERGVPVNAVDGKGRTPLHLA
                     VKACVDSYWTERRTPEP"
     gene            complement(1210..2145)
                     /locus_tag="AC16JC_08495"
     CDS             complement(1210..2145)
                     /locus_tag="AC16JC_08495"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019403.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase domain-containing protein"
                     /protein_id="HIR5164773.1"
                     /translation="MVLELTLLRVAWTFNLDFAHYEMAGVIWAIGWCMILMAGLVRLP
                     LPAVGAIGVIIIAAHNLLDPHLGALVPTLGNDLRGGLWKVLYVGFFAGPIQFGPDGPN
                     LIVLYSIVPWIGVMAAGYAFGTVLTLEPARRNRICLMLGLGATALFLLLRSFNLYGDP
                     RPWNAAPRPDGPPPMPALLAFLNTNKYPASLLFLLMTLGPTLALVPLLERVRGTAARF
                     VTVFGRVPFFYYMLHIPLIHALALVVSKVRLGAVSPWLFTNHPMGNPPPPEGYVWSLP
                     LLYLVWAVAIAMLYVACRWFADLKARRKDWWLKYL"
     gene            complement(2130..2432)
                     /locus_tag="AC16JC_08500"
     CDS             complement(2130..2432)
                     /locus_tag="AC16JC_08500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164774.1"
                     /translation="MSATVPTGVAAPAAAASRIASVDLIRGAVMILMAIDHVRVFSSL
                     PAGGPTPGIFFTRWVTHFCAPAFIFLAGPARSSTAAGIRIFPGSCSSAAPGSWCSS"
     gene            complement(2500..3213)
                     /locus_tag="AC16JC_08505"
     CDS             complement(2500..3213)
                     /locus_tag="AC16JC_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR5164775.1"
                     /translation="MAEIRALIVDDEPLARRGIRQLLVPYRDVVVVGECRNGREAVRA
                     LAETRPDLVFLDVAMPGLDGFGAIRVHGVERMPLTVFVTAHDEFAVRAFEADALDYLV
                     KPLSEARFRTTMTRVLHRLATRARTPIAVPTGRGELLLDPDEIDWIGARDDYAEIHAG
                     AKTYRVRTTLGALVSRLDAARFVRVHRSAIVCVDRVRELNTARGRAETVVRLRDGTEV
                     PVSRRRLSTVKASIRPPRR"
     gene            complement(3206..4213)
                     /locus_tag="AC16JC_08510"
     CDS             complement(3206..4213)
                     /locus_tag="AC16JC_08510"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5164776.1"
                     /translation="MQGRLGSREPATWRSLVWEGGDWLICALLTPAVFWLAHRLPLSR
                     ETVTRRVPQHLLAAILFCVAWAGAGVLLSRAMFRSTPYGGGALSWFFTTLPFGVAVYF
                     ALLGVAHAAAYFLEARERETQAARLSAQLAEARMGALRMQLQPHFLFNSLNAIAVVVR
                     DRDTATATRLLDQLGDLLRRVMRSDRPPEVTLAEELDFVRQYLAIEQVRFSDRLRPVF
                     AADESLLAAAVPEFVLQPLVENALRHGLARRTTATLLRIEARREGDDLLLMVTDDGPG
                     PDDAAPEPAAGVGLGNLRERLATLYAERARLELRPTPQGGAVATVRLPYHELHPETPR
                     G"
BASE COUNT          719 a         1498 c         1587 g          581 t
ORIGIN      
        1 ccggctcggg cgtgcgccgt tccgtccagt aggaatccac gcaggctttc acggccaggt
       61 gtagcggcgt ccgccctttg ccgtccacgg cgttcaccgg cacgccgcgt tcgagaaaca
      121 actgcaccag ctcggtgttc agcatccagg ccgccacgtg cagcgcggtg ctgtcgccgg
      181 cgatgtcgaa gtacccgtag ccgtcgtagc gcgccgtggc cggcacgccg agctcgagca
      241 gcagccggac gccggcgttg ttccaggtca gggtgaactc cgacagcagc gtggccccgt
      301 cggcgagcac ctcgcgcaac agggcgggct cgtcctgcgc gattgcgtgt gcggcggccg
      361 cgtcggcgcg ggccagggcg gcgtgcagcc gttcgacccc gcgaagcacg agcgggacgc
      421 cgcgtcgctc gagtgccgcg aggacgtcgc tccggccctt gcggccggcg agggcgatcg
      481 ccgagcggcc ctcgtcgacc tcgttcgggt ctgcgccgtt gtcgagcaac agctcgacga
      541 tctggagact gttgtcgcgc tcgagagcgt gatgcagcgc ggggctgccg cgcgatcgct
      601 tgcccgtgag gttggcgcct cgggccagca gatacctgac tccctcgaag tcgtgccagt
      661 cgagcttgcg gatcagcatc agcagcaggt tctcgggcgt cacccgcccg gtctccacca
      721 gcaccttcaa agcgccaagg tctcgacctt cgggcgcgtg atacacggca tctacgtcgt
      781 tgggatctgc gccgcgctcg atcagcagcc gggtgagcgg ctcgttgtgc gcgacgcccg
      841 cggcgccgta gagcgcggtc tcgaactcgg ggtattgccc tgtggtccag aacccggtgt
      901 gcgccggcgc cccggcatcg agcagtgcgg tggccgtgcg cacaaagtcc tcggtgcgct
      961 cgggatggag tcggaggtat ttggaaagac ccagatagtt gaggggatcg ccgccccacg
     1021 ggggcgactg cgccgtcacg ctcgccgggt cgcgcgcgat gaagcgccgc acggcggcct
     1081 cgtcgccgag gatcgccgcg atgtggatgt ccgcgctgtg cagctcggga tgctcggcca
     1141 gaatcgcctc ggcgcgctcc agcatgccgt gccagcacgc ggcctcgatg aaggcgaccc
     1201 gggggtccgc tacaggtatt tgagccacca atccttccgc ctggccttga gatcggcgaa
     1261 ccagcggcac gcaacataga gcatcgcgat cgccacggcc cagaccagat acagcagcgg
     1321 caggctccag acgtacccct cgggcggcgg aggattgccc atggggtgat tggtgaacag
     1381 ccacgggctc accgcgccga gccggacttt cgataccacg agcgccagcg cgtgaatcag
     1441 cggaatgtgg agcatgtaat agaagaacgg cacgcggccg aacacggtga cgaaccgcgc
     1501 cgccgttccg cgcacgcgtt ccagcagcgg gaccagcgcg agcgtcggtc ccagcgtcat
     1561 caggagaaac agcagagacg ccgggtactt gttggtgttg agaaaggcga gcagcgcggg
     1621 catcggcggc gggccgtcgg gccgcggcgc ggcgttccag gggcggggat cgccatagag
     1681 gttgaagctc cgcagcagca aaaagagtgc ggtggctccg agtcccagca tcaggcaaat
     1741 ccgattgcgc cgcgccggct cgagcgtcag caccgtgccg aaggcgtagc ccgcggccat
     1801 gacgccgatc cacggcacga tcgaatagag cacgatgagg tttggtccgt ccggcccgaa
     1861 ctggatcggc ccggcgaaga accccacata gaggaccttc cacagcccgc cgcgcaggtc
     1921 gttgcccagc gtcgggacga gcgcccccag gtgtgggtcg agcaggttgt gggccgcgat
     1981 gatgatgacg ccgatcgctc cgacggccgg cagcggcagc ctgacgagcc ccgccatcag
     2041 gatcatgcac cagccgatgg cccagatgac gcccgccatc tcgtaatgtg cgaaatcgag
     2101 gttgaacgtc caggcgacgc gcagcagggt cagctcgagc accacgagcc aggcgccgcg
     2161 gacgagcagg aaccgggaaa gatccggatg ccggcggccg tagaggaacg cgctggcccg
     2221 gcgaggaaga tgaaggccgg tgcgcagaaa tgcgtgaccc agcgggtgaa gaagatcccg
     2281 ggcgtggggc cgccggcggg gaggctggag aacacccgca cgtggtcgat cgccatcagg
     2341 atcatcacgg cgccacggat cagatcgacg gacgcgatgc ggctcgccgc cgccgcgggc
     2401 gccgcgaccc cggtaggaac ggttgcgctc atggtcccaa cgtcgtaggc ggggcgcgcg
     2461 ccgtcaaatc gcgtgcggcg agcggtcgat acgatgcggt caccggcgtg gtggccggat
     2521 actggctttc accgtggaga ggcggcgccg gctgacgggc acctcggtcc cgtcgcgcag
     2581 gcgcacgacg gtctcggcgc gcccgcgcgc cgtgttcagc tcccgcaccc ggtccacgca
     2641 cacgatggcg gagcggtgga cccgtacgaa gcgcgcggca tcgagtcgcg acaccagcgc
     2701 accgagcgtc gtgcgcacgc ggtacgtctt cgcgccggcg tgaatctcgg cgtaatcgtc
     2761 ccgcgccccg atccagtcga tctcgtcagg atcgagcaac aactcgccgc gcccggtcgg
     2821 cacggcgatc ggggtgcggg cgcgggtggc cagccggtgg agcacccggg tcatggtggt
     2881 gcgaaagcgc gcctcggaca gcggcttcac cagatagtcg agcgcgtccg cctcgaacgc
     2941 ccgcaccgcg aactcgtcgt gggcggtgac gaacaccgtg agcggcatgc gctcgacgcc
     3001 gtgcacccgt attgcgccga atccgtcgag gcccggcatc gccacgtcca gaaagacgag
     3061 atcgggtcgg gtctcggcca gggcacggac cgcctcgcgt ccgttacggc actcccccac
     3121 cacgacgacg tcacgatacg gcaccaggag ctgacggatg ccgcggcgcg cgagcggctc
     3181 gtcgtccacg atcagggcgc ggatctcagc cacggggagt ctcggggtgc agctcgtggt
     3241 agggtaaccg caccgtggcc accgctcccc cctgcggcgt cggccgaagc tcgaggcgcg
     3301 cccgctcggc gtacagcgtg gcgagccgct cgcgcaggtt gcccaacccc acgcccgcgg
     3361 cgggctcggg cgccgcgtcg tcggggcccg gcccgtcatc ggtgaccatg agcagcaggt
     3421 cgtccccctc acgccgcgct tcgatccgca gcagcgtcgc ggtcgtgcgc cgggcaagcc
     3481 cgtgccgcag cgcgttctcg accaggggct ggagcacgaa ctcgggcacg gccgcggcga
     3541 ggaggctttc gtccgcggcg aagaccggcc gcagccggtc ggagaatcgc acctgctcga
     3601 tcgcgaggta ttggcgcacg aaatccagct cctccgccag cgtcacctcc ggcgggcggt
     3661 cactgcgcat cacgcggcgg agcaaatccc ccagttggtc cagcaggcgc gtcgcggtcg
     3721 ccgtgtcgcg gtcgcgcacg accacggcga tggcgttgag actgttgaag aggaagtgcg
     3781 gctgcagctg catgcggagc gcgcccatgc gggcttccgc gagctgcgcc gacaggcggg
     3841 ccgcctgcgt ttcccgctcg cgcgcctcca ggaagtaggc ggcggcgtgc gcgacgccca
     3901 gcagggcgaa atacaccgcg acgccgaagg gcagggtagt gaagaaccag ctgagcgctc
     3961 ccccgccgta gggcgtggag cggaacatcg cccggctcag gagcaccccg gcgccggccc
     4021 aggcgacgca gaacaggatc gccgcgagga ggtgctgggg cacccgacgg gtcaccgtct
     4081 cccgggagag cggcaggcgg tgcgcgagcc agaagaccgc gggcgtgagg agcgcgcaga
     4141 tgagccagtc gccgccctcc cacacgaggc tgcgccacgt cgcgggctcg cggctgccga
     4201 gccgaccctg cacgtacgcc tcgaaggcgg cgaggatcgc ggggccgagc caggcggcgg
     4261 agacgaaaac ccagatcgga ggtcgcagga tgccataagc tgcgccctgg tcctgccgcg
     4321 gacaagagat gttgggtggc ggcgcgcgtc atcccggcgc gccgccatgt cacgcggtca
     4381 attcg
//
LOCUS       DBNGZW010000362         2613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_170586, whole genome shotgun sequence.
ACCESSION   DBNGZW010000362 DBNGZW010000000
VERSION     DBNGZW010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2613
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_170586"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..583)
                     /gene="ftsE"
                     /locus_tag="AC16JC_08515"
     CDS             complement(<1..583)
                     /gene="ftsE"
                     /locus_tag="AC16JC_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR5164777.1"
                     /translation="MIKFTSVFKTYAKGGLALKDVSFHIAKGEFTFLTGHSGAGKSTV
                     LKLVYADERPSSGEVRVSGYDVTSIGARDVPHLRRRLGIVFQDFRLLEDRTAEENVAF
                     ALEVTGARRSAIPARVMRVLTQVGLAAKAGAYPRELSGGEQQRVAIARALVNEPQVLL
                     ADEPTGNLDERATRGVFQLLRDINASGTAVVMAT"
     gene            complement(584..1741)
                     /locus_tag="AC16JC_08520"
     CDS             complement(584..1741)
                     /locus_tag="AC16JC_08520"
                     /inference="COORDINATES: protein motif:HMM:NF013237.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR5164778.1"
                     /translation="MTRRRLGLSTRAIHGEPEPRPDWTPISQPIYQSSTFTNPVGSSA
                     EVLYTRYGNNPNQVQIGRRLARLEGAEAALFVASGMGATALAHLAVLRPGDHLISSEW
                     IYGGVHKLFTEEFGRFGIEVTFVDPLRSRAWKEALRKNTRAMFVETPTNPLLRVIDLE
                     PIATLAKAEGLALLVDSTFASPIAFRPLEHGADIVIHSATKYLNGHTDVIAGAVAGTE
                     SVVEEVRRLMQLWGQAIDPHAAWLIERGMRTLAIRVARHSATGLAVAEWFSRQPQVAV
                     VHYPGLSSHRDHKTAKRVLEGYGGMLGLELKGGARAAERFLRALTIAAHAPSLGGVET
                     LVSEPRLTSHKSLTADERARAGIPDGFLRFSLGLEDADDLIADFAQALAQL"
     gene            complement(1738..2175)
                     /locus_tag="AC16JC_08525"
     CDS             complement(1738..2175)
                     /locus_tag="AC16JC_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164779.1"
                     /translation="MIQSIDVSAVLRLAVRATYADLVTRATGRAVREHIEQAYAADAG
                     FVARMDFTGVRCIDYSCADEIVAKLLRERIHVLVLRGCSDAHLEALGPVLEGHGLAAL
                     LERADGTLDGLGRPESTEALLDELVDRRLAARTAGGAVALVAA"
     gene            complement(2172..>2613)
                     /locus_tag="AC16JC_08530"
     CDS             complement(2172..>2613)
                     /locus_tag="AC16JC_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164780.1"
                     /translation="LSEACQNIVEHAGTGGWVAVQSYHWRRRLARRVVVIAVADAGVG
                     FRASLEAAHGRRFGDRWSDAAALEAALIQGVSRFRDPGRGQGLKGIRNYVARWDAKIA
                     IRSGTARIAIVPSWDDDVPLAEGLPPFPGSQVLLIIPEQRARHT"
BASE COUNT          398 a          939 c          890 g          386 t
ORIGIN      
        1 gggtcgccat gaccaccgcc gttccgctgg cattgatgtc gcgcagcagc tggaacacgc
       61 cccgcgtggc gcgctcgtcg agattgccgg tgggctcgtc cgccaggagg acctgcggct
      121 cgttcaccag ggcccgggcg atcgccaccc gctgctgctc gcctccggac agctcacgag
      181 gatacgcccc ggccttggcg gccagcccca cctgcgtgag cacgcgcatc acgcgggcgg
      241 gaatcgcgga gcgccgcgcc ccggtcacct ccagggcgaa cgcgacgttt tcctcggcgg
      301 tgcggtcctc cagcagccgg aaatcctgaa acacgatccc caggcggcgg cgcaggtgcg
      361 gcacgtcgcg ggcgccgata ctcgtcacgt cgtaccccga cacgcgcacc tcgccgctcg
      421 agggtcgctc gtcggcgtag acgagtttca ggacggtgga tttgccggcg ccggagtgac
      481 cggtgaggaa ggtgaactca cccttcgcga tgtggaagga gacgtccttg agcgcgagtc
      541 cacccttcgc gtacgtcttg aagacgctcg tgaacttgat cacctagagc tgtgccagcg
      601 cctgggcgaa atccgcgatg aggtcgtcgg catcctcgag tccgagggaa aatctcagga
      661 acccgtccgg aatgccagcc cgggcgcgtt cgtcggccgt gaggctcttg tgggacgtga
      721 gccgtggctc ggagaccagc gtttccacgc cgcccagcga cggggcgtgc gcggcgatcg
      781 tgagggcccg caggaaccgc tcggcagccc gcgcgccacc cttgagctcg aggcccaaca
      841 tcccgccata cccttcgagt acccgtttgg ccgtcttgtg atcgcggtgc gaggacagcc
      901 cggggtagtg caccacggcg acctgcggct ggcggctgaa ccactccgcc acggcgaggc
      961 ccgtcgcgga gtgccgcgcc acacggatgg cgagcgtccg catgccgcgc tcgatcagcc
     1021 aggcggcgtg cgggtcgatc gcctgacccc acagctgcat cagccgccgg acttcctcga
     1081 cgacggactc cgtgcctgcg acggcgccgg cgatcacgtc ggtgtgccca ttcaggtact
     1141 tcgtcgcgct gtggatcacg atgtccgccc cgtgctccag cggccggaac gcgatcgggg
     1201 aggcgaaggt ggagtccacg agcagggcca gcccttcggc cttcgccagc gtcgcgatcg
     1261 gctcgaggtc gatgacgcgg agcagcgggt tcgtcggcgt ctccacgaac atcgcccggg
     1321 tgttcttgcg cagcgcttcc ttccaggcgc gggagcgcag cgggtccacg aaggtgacct
     1381 cgatcccgaa gcgcccgaat tcctcggtga acagcttgtg cacgcccccg tagatccact
     1441 cgctggagat cagatgatcc cccggccgca atacggcgag gtgtgcgagc gcggtggccc
     1501 ccatcccgct cgccacgaac agcgccgcct cggcgccctc cagccgggcc agccgccgcc
     1561 cgatctgcac ctggttgggg ttgttgccgt atcgggtgta gaggacttcg gcggacgagc
     1621 cgaccggatt ggtgaacgtg ctgctctggt aaatcggttg ggagatcggc gtccaatcgg
     1681 gtcgcggctc cggctcgccg tgaatggcgc gagtggagag gccgagccgg cgacgcgtca
     1741 cgcggcgacc agcgcgaccg cgccccccgc cgtccgcgcg gccagccggc ggtcgaccag
     1801 ctcgtcgagc aacgcctcgg tcgattctgg ccgccccagc ccgtcgagcg tcccgtcggc
     1861 ccgctccagc agggccgcca ggccgtgtcc ttccaggacg ggcccgagcg cttccaggtg
     1921 ggcgtccgag caaccgcgca gcaccagcac gtgaatccgc tcccgcagca gcttggccac
     1981 gatctcgtcg gcgcaggagt agtcgatgca gcggacaccg gtgaaatcca tccgggccac
     2041 aaacccggca tcggcggcgt acgcctgctc gatgtgctcg cggaccgcac ggcccgtggc
     2101 ccgggtgacg aggtccgcgt acgtggcgcg caccgcgagg cgcagaacgg cggaaacatc
     2161 gatggactgg atcacgtgtg tcgggctctc tgctccggga tgatgagaag cacctgggag
     2221 ccggggaagg gcggcaaccc ctcggcgagc ggcacgtcgt cgtcccagga tgggacgatg
     2281 gcgatgcgcg cggtgccgct acggatggcg atcttggcgt cccaccgcgc gacgtagttc
     2341 ctgatccctt tcagcccctg cccgcggccg gggtcccgga atcggctcac cccctggatc
     2401 agcgccgctt ccagcgccgc cgcatcgctc cagcggtcgc cgaagcggcg cccgtgcgcc
     2461 gcctcgagtg aagcacggaa ccccacgccc gcatcggcca ccgcgatgac caccacgcgg
     2521 cgcgccagac ggcgtcgcca gtggtaactc tgcactgcca cccagccgcc cgtcccggcg
     2581 tgctctacaa tattctgaca cgcctccgaa agc
//
LOCUS       DBNGZW010000363         1550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1387786, whole genome shotgun sequence.
ACCESSION   DBNGZW010000363 DBNGZW010000000
VERSION     DBNGZW010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1387786"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1331)
                     /locus_tag="AC16JC_08535"
     CDS             complement(<1..1331)
                     /locus_tag="AC16JC_08535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF051540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR5164781.1"
                     /translation="MPDRRRVGRSTLPDQRTLLSWLFVGRLVLSVGILLGAGLVWTER
                     PQESFLVTAAVVVALLFTGLGAWRVFLRNGPVGNGFLLVQALVDLGVITTIVHFAAQP
                     QSAFPALYVLAVAAYALLMPPTWGALTAVVASALFLGDAFWTRAGAPDAAFWAQVVVF
                     NMVFAIVAVLGHRLREAGMEQETLAIELKRVRLEADDILRNIRSGVLTVDGLGRLAFI
                     NPTGQKLLDLDGEGLIGMPVLDQLKTRSSELWAAVVAGIRNGRKISRGEGMVMHHGGR
                     IFPIGLSTTTFRQEAQEAPSVTAIFTDISDLKEVQELQARAERLESVAALSASLAHEI
                     RNPLASIRSSVEQLARSKHADEDERFLAGLIVRESDRLSRLLSEFLDFARVRATQLVP
                     LDLHAVVVAVVRLIRAHPDCRPDAVLTIEGGRTMLDGDEDLLHRVIANLVL"
     gene            1409..>1550
                     /locus_tag="AC16JC_08540"
     CDS             1409..>1550
                     /locus_tag="AC16JC_08540"
                     /inference="COORDINATES: protein motif:HMM:NF013691.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HIR5164782.1"
                     /translation="MAWHVYVARCGDGTLYTGITTDPARRESAHNTGRGGAYTRSRRP
                     VRL"
BASE COUNT          256 a          552 c          540 g          202 t
ORIGIN      
        1 ttgagcacca ggttggcgat gacccggtgc agcaggtcct cgtcgccgtc cagcatggtc
       61 cgcccgccct cgatcgtgag cacggcgtcg ggacggcagt cgggatgagc gcggatcagc
      121 cggaccaccg ccactaccac cgcgtggaga tcgagcggca cgagctgggt ggcgcgcacc
      181 cgggcgaagt cgaggaactc gctcagcagg cggctcagcc ggtcgctctc gcggacgatg
      241 agcccggcga ggaagcgctc gtcctcgtcg gcgtgcttgg agcgggcgag ctgctcgacc
      301 gagctccgga tcgacgccag cgggttccgg atctcgtgcg ccagggacgc gctcagcgcc
      361 gccaccgact ccagccgctc ggcgcgggcc tggagttcct gcacttcctt gaggtcggag
      421 atgtcggtga agatggccgt caccgacggc gcctcctgcg cctcctgacg gaacgtggtg
      481 gtgctcaggc cgatcgggaa gatgcgcccg ccgtggtgca tcaccatgcc ttcgccccgg
      541 ctgatcttcc ggccgttgcg gatgccggcc accaccgccg cccagagctc cgacgagcgg
      601 gtcttgagct ggtccagcac cggcatgccg atcagcccct cgccgtcgag gtcgagcagc
      661 ttctggcccg tcgggttgat gaaggcgagc cgccccagcc cgtcgaccgt cagcaccccc
      721 gaccggatgt tccgcaggat gtcgtccgcc tctagccgga cgcgcttgag ctcgatcgcg
      781 agcgtctcct gctccatgcc cgcctcgcgc aggcggtggc ccagaaccgc gacgatcgcg
      841 aacaccatgt tgaacaccac gacctgggcc cagaacgccg cgtccggggc gccggcccgg
      901 gtccagaacg catcgcccag gaacagcgcc gacgcgacga cggcggtgag cgccccccag
      961 gtgggcggca tgaggagcgc gtaggccgcg acggccagca cgtacagcgc ggggaacgcc
     1021 gactgcggct gggcggcgaa gtggacgatc gtcgtgatga cgccgaggtc caccagcgcc
     1081 tgcaccagca ggaagccatt cccgaccggc ccgttccgaa ggaagacccg ccaggcgccc
     1141 aggccggtga acagcagggc gacgacgacg gcggcggtga cgaggaacga ctcctggggc
     1201 cgctcggtcc agaccaggcc ggcgccgagc aggattccca ccgagagcac cagccgcccg
     1261 acgaacagcc aggacaacag ggtgcgctgg tcgggcaggg tgctgcggcc gacccggcgg
     1321 cgatcaggca tcgtctccta ctggactcgg cgtatgcaca aggggttgtg gccgaagata
     1381 gaaatgcggc cctatcatta caatccatat ggcatggcac gtctacgtcg cccgctgcgg
     1441 cgacggcacc ctctacaccg ggatcacgac cgatccggcc cggcgggagt cggcccacaa
     1501 cacaggccgc gggggcgcct atacccgctc gcgccgcccg gtgcggctga
//
LOCUS       DBNGZW010000364         4569 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_706460, whole genome shotgun sequence.
ACCESSION   DBNGZW010000364 DBNGZW010000000
VERSION     DBNGZW010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_706460"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /locus_tag="AC16JC_08545"
     CDS             complement(<1..1373)
                     /locus_tag="AC16JC_08545"
                     /inference="COORDINATES: protein motif:HMM:NF014428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5164783.1"
                     /translation="MRSDRSALLAIFVAVLGCSDNSGPTVHYLLAITGGDAQTDTVGK
                     VLATPYAVKVTDQAGALAAGVTVSWIVTAGGGSITPTSTTDAAGIATATRTLGTVSGA
                     ATAAAQAGGASVVFSATALADPPAHMLKSAGDVQTGATGAALPVPFAVLVQDQYNNPV
                     ESVTVNWAVTVGSGTLSVTSSKTDGLGVARTTLTLGDTAGPNAVTATSVGLGAPLTFT
                     ATGSAGPVLVKLLPIPQNYGIHDQFIRDGLAFVCAWNSGIIIYDVGNGIKGGSPANPV
                     LVDSITTAGGEVHNAWWFHNPAIPEQKYLFIGQEGSGSIGTSSSGDIHVVDVSDLNHI
                     HEVGFFHRDGAGTHNFWMDESKAILYAAYYNGGVVAIDVSGTLSGDISSRMIDTLSFG
                     VGNTYTWGVQLYNSSLYAMDMVSGFWQLNTANGALSVAAGGNNVPERFGSDLWVANGY
                     AYTGTWG"
     gene            complement(1462..2640)
                     /locus_tag="AC16JC_08550"
     CDS             complement(1462..2640)
                     /locus_tag="AC16JC_08550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164784.1"
                     /translation="MTAEWGVQAIPALTETNVVPGGRSLGELRVVQPVAMLHAATLGN
                     RLRLIGIVDFEKYTIPHGELSPGSWGESFVDRRHPHTLVHELMLVGGGRRLFAAAGKG
                     FVPFGTDDPMSRPTVRFPVNHHLSQILERAVLLAGVSVGPAMLEGALFNGDEPVSPGS
                     WPAWDRFGDSWALRLSAYPVTGLEAQVSYAYVKSPENRPGAAPADRKTSLSTRWERGR
                     WYGEVEWARTATADGFFVFHSVLAEGAVALARHRPYVRLERSERPEEERLFSSLFRSR
                     RPLFENSILGTTRWTVLTAGDALDLKWGRLRTTPFVEASWIGIARVGGGVFDPASFYE
                     REHGAAVTIGIRLATGMRMPRMGRYGYQQEPGMHGMHMGSRRSTFPGVETPARPGLRP
                     "
     gene            complement(2709..3986)
                     /locus_tag="AC16JC_08555"
     CDS             complement(2709..3986)
                     /locus_tag="AC16JC_08555"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR5164785.1"
                     /translation="MGPGPHPAALTGATPAEAARLIVSAVRPLPAELRTLRDALDLVT
                     ADDISSPIDLPPWTNSAMDGYACRTADVRAGVVLRVVETIAAGQFPQHALAAGEAARI
                     FTGAPLPTGADGVIRQEDTEADGDRVTIRDPRDAGRNIRRTGEDIRRGALVLPAGTPL
                     GPAQLGVLASIAREPVAVHRQPIVAFLGSGDEIVDLDRRDEILAGKKIATSNSYTLSG
                     MIRRAGAVPRELGIARDTVQSLRDHIRRAAGSDLLVTTAGVSVGEHDLVRDVLTELGC
                     DLRLWRVRMRPGSPLGFGVLPPPLGPLPWIGLPGNPVSTMVTFELFVRPIIRRLLGHA
                     RPFRRTVRVIVEDEITLGPRLRHFLRAIVEARPDGALGARLTGPQGSGILTSMARADA
                     LLIVPEDRPHVSPGESLSALVLDDPHPTAEPEF"
     gene            complement(3956..4486)
                     /gene="mobB"
                     /locus_tag="AC16JC_08560"
     CDS             complement(3956..4486)
                     /gene="mobB"
                     /locus_tag="AC16JC_08560"
                     /inference="COORDINATES: protein motif:HMM:NF015181.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HIR5164786.1"
                     /translation="MAATRIISIIGKKDAGKTSLVVALATELRRKFRVMTLKHGTHAA
                     DLDQRGKDTWRHWHEGKAERVLLEAPGARVLFERTDHEDDPITLTRRYLDGADIVLVE
                     GFSAHPLPKIEVFRTAVGDAPIYDPTRDNADLWVAMITDSPTFRAPFPVFRFSDTSWL
                     VALANLAWDRARILPP"
BASE COUNT          693 a         1554 c         1630 g          692 t
ORIGIN      
        1 aagccccacg tgccggtgta ggcgtatccg ttcgccaccc acaggtccga gccgaagcgc
       61 tcggggacgt tgttcccgcc ggccgcgaca ctcagcgcgc cgttcgcggt gttgagctgc
      121 cagaagccgg ataccatgtc catcgcgtac agcgagctgt tgtagagttg cacaccccag
      181 gtgtaggtgt tgccgacgcc gaagctcagc gtgtcgatca tgcgcgagga aatgtccccg
      241 gagagggttc ccgacacgtc gatcgccact accccgccgt tgtagtaggc ggcgtacagg
      301 atcgccttcg attcgtccat ccagaaattg tgcgtccccg cgccgtcacg gtgaaagaat
      361 ccaacttcgt ggatgtggtt cagatccgag acgtccacca cgtggatgtc gcccgaggag
      421 gaggtgccga tggagcccga gccctcctgc ccgatgaaca ggtacttctg ctcggggatc
      481 gcggggttgt ggaaccacca ggcgttgtgg acttcgccgc ccgccgtggt gatgctgtcc
      541 accagcaccg ggttcgccgg cgagccgccc ttgatgccgt tcccgacatc atagatgatg
      601 atgccgctgt tccacgcgca cacgaatgcc aggccgtcgc ggatgaactg atcgtggatc
      661 ccgtagttct gcggaatggg aagcagcttg accagtaccg gtccagcgga gcccgtggcc
      721 gtgaaggtga gcggcgcgcc cagtccgacc gacgtcgcgg tcaccgcatt cgggccggcc
      781 gtgtcgccca gtgtcagcgt ggttcgcgcc acgccgaggc catcggtctt cgagctggtc
      841 accgagagcg tgccgctccc gaccgtgacc gcccagttca ccgtcacact ctcgaccggg
      901 ttgttgtact ggtcctggac gaggaccgcg aacggcaccg gcagggccgc gccggtcgct
      961 ccggtctgca cgtcgccggc gctcttcagc atgtgagccg gcgggtcggc gagggccgtg
     1021 gcgctgaaga cgaccgaggc gcccccggcc tgcgccgcgg cggtagcggc gccggacacg
     1081 gtgcccagtg tgcgcgtggc ggtcgcgatg cctgcggcgt ccgtcgtgga ggtgggcgtg
     1141 atgctgccgc cgcccgccgt gacgatccag gaaacggtga cccccgccgc gagcgcgccc
     1201 gcctgatccg tcaccttgac cgcgtagggg gtcgccagaa ccttgccgac ggtgtcggtt
     1261 tgcgcgtcgc cgccggtgat ggcgagcagg tagtgcacgg tgggtccgga gttgtcgcta
     1321 caaccgagaa ccgccacgaa tatcgcgagc agggcagatc tatcggatcg catctttcct
     1381 caagttcgct atcgaatcgg tgcaggtgcg gtccggatct tcccgtgccc tgaagggccg
     1441 gctcggcgcg atcacgcgtc cttaaggacg caacccgggc cgagccggcg tttcaacgcc
     1501 cgggaacgtg ctgcgtcgtg atcccatatg catgccatgc atccccggct cctgctggta
     1561 cccgtagcgg cccatccggg gcatgcgcat cccggtggcc aggcgtatgc cgatggtgac
     1621 ggcggccccg tgctcccgtt catagaagga tgccggatcg aataccccgc cgccgacccg
     1681 cgcgatcccg atccatgacg cttccacgaa tggcgtggtc ctcagtcgtc cccatttgag
     1741 gtcgagtgca tcgcccgccg tcagcaccgt ccaccgggtg gtgcccagga tcgagttctc
     1801 gaacagcgga cggcggctgc ggaagaggct ggagaacagc cgctcctcct ccggccgctc
     1861 gctgcgctcg agccgtacgt agggacggtg tctcgccagg gcgaccgctc cctccgccag
     1921 cacggaatga aacacgaaga acccgtcggc ggtggcggtc cgtgcccact cgacctcacc
     1981 ataccagcgg ccgcgctccc agcgcgtcga gaggctcgtc ttgcggtcgg cgggagccgc
     2041 tcccgggcgg ttctcgggcg acttcacgta ggcataggaa acctgcgcct cgagtcccgt
     2101 gaccgggtac gcgctgaggc gcagcgccca cgaatcgccg aagcggtccc aggccggcca
     2161 cgagccgggc gacacgggct catcgccgtt gaacagcgcg ccctcgagca tggcgggccc
     2221 gacgcttacg cccgcgagca gcacggcgcg ctcgaggatc tggctcaggt ggtgattgac
     2281 ggggaagcgc accgtggggc gcgacatggg atcgtcggtg ccgaagggca cgaacccctt
     2341 gccggcggcg gcgaacaggc gccgcccgcc gcccaccagc atcagctcgt gtacgagagt
     2401 atgggggtgg cggcggtcca cgaagctctc gccccagctg ccgggagaca gctcgccgtg
     2461 cgggatcgtg tacttctcga aatccacgat gccgatgagc cgcagccggt taccgagggt
     2521 cgcagcgtgc agcatcgcta ccggctggac cacccgcagc tcacccagcg atcggccccc
     2581 cggcacgacg ttggtctccg tgagcgccgg aatcgcctgc acgccccatt cggccgtcac
     2641 gcgctgcgcc accgcacggc cggggaggag cagccccggg atgatcaggt accaacacgt
     2701 tgccggtctc agaactccgg ctccgcggtg ggatggggat cgtcgagcac cagcgcggac
     2761 agcgactctc ccggtgagac gtgcggccgg tcttcgggaa cgatgagcag cgcgtcggcc
     2821 cgggccatcg aggtgagaat ccccgatccc tgcggaccgg tgagccgcgc gccgagcgca
     2881 ccgtccggtc gggcctccac gatggcccgc aggaagtggc gcagcctggg acccagggtg
     2941 atctcgtctt ccactatcac gcgcaccgtg cggcggaacg ggcgggcgtg tcccagcagg
     3001 cggcggatga tggggcgcac gaacagctcg aaggtgacca tagtgctgac gggattgccg
     3061 gggagaccga tccagggcag ggggcccagg ggagggggca gcacgccgaa ccccagcggg
     3121 gatcctgggc gcatgcggac gcgccacagg cgcaggtcgc aacccagctc ggtcaggacg
     3181 tcgcgcacca gatcgtgctc gcccacgctg acgccggccg tcgtgacgag caaatccgag
     3241 ccggcggcgc gccggatgtg gtcgcgcagc gattgtaccg tgtcgcgcgc gattcccagc
     3301 tcgcgcggca ccgcgcccgc gcgccggatc atccccgaca gggtgtagga attggaggtc
     3361 gcgatcttct tgccggcgag gatctcgtcg cggcggtcga ggtccacgat ctcgtcgccc
     3421 gagcccagga atgccacgat cggctgacga tgcacggcca ccggctcgcg ggcgatggag
     3481 gcgagcacgc ccagctgggc cgggccgagc ggcgtgccgg cgggcagcac cagggctcca
     3541 cggcggatat cctcgcccgt ccggcggatg ttccgacccg cgtcgcgcgg gtcgcggatc
     3601 gtgacgcggt cgccgtcggc ctcggtgtcc tcctgccgga tgaccccgtc cgcgcccgtg
     3661 gggaggggag cgcccgtgaa gatgcgcgcg gcttcgcccg cggcgagtgc gtgctgcggg
     3721 aactgaccgg cggcgatggt ctccacgacg cgcagcacga cgccggcgcg cacgtcggcc
     3781 gtgcggcagg cgtagccgtc catggccgag ttggtccacg gggggaggtc gatcgggctc
     3841 gagatgtcgt ctgccgtgac gaggtcgagc gcgtcgcgca gcgtccgcag ctcggccggg
     3901 aggggccgga cggcggagac gatcagccgc gcggcctcgg ccggcgtggc gccggtcagg
     3961 gcggcaggat gcgggcccgg tcccacgcga ggttggcgag cgccacgagc cagctcgtgt
     4021 cgctgaagcg gaacaccggg aacggggcgc ggaatgtcgg gctgtcggtg atcatcgcga
     4081 cccacaggtc ggcgttgtcg cgcgtcgggt cgtagatcgg cgcgtccccg accgcggtgc
     4141 ggaacacctc gatcttgggt agcgggtggg cggagaaccc ctcgacgagc acgatgtcgg
     4201 cgccgtcgag ataacgccgg gtcagggtga tcgggtcgtc ctcgtggtcg gtgcgctcga
     4261 acagcacgcg cgcaccgggc gcctcgagca gcacgcgctc cgccttgccc tcgtgccaat
     4321 ggcgccaggt gtccttgccg cgctggtcca ggtcggcggc gtgggtgccg tgcttgagcg
     4381 tcatgacgcg gaacttgcgg cgcagctcgg tggccagcgc caccacgagc gacgtcttgc
     4441 cggcgtcttt cttcccgatg atcgagatta tgcgcgtcgc cgccacaaca cctccgcgcc
     4501 caagtcgcgg ctgaagccgc ggctcggctg ggctgtcact tacagtgact cgagccgcag
     4561 gtggatctg
//
LOCUS       DBNGZW010000365         2897 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_512883, whole genome shotgun sequence.
ACCESSION   DBNGZW010000365 DBNGZW010000000
VERSION     DBNGZW010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2897)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2897)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2897
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_512883"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..666)
                     /locus_tag="AC16JC_08565"
     CDS             complement(58..666)
                     /locus_tag="AC16JC_08565"
                     /inference="COORDINATES: protein motif:HMM:NF016398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HIR5164787.1"
                     /translation="MRDLKELQIDALREVANIGAGHAATALSQMTNRRIMISVPQINI
                     ARLEEVADLLGDAQAVVVAVLMHMLGDLTGRTLLLFPEKVGQRLCDMLLRRPSGATTG
                     FGALEQSALKEAGNILSGAYMNALSDFMGMLLLPSVPSLVIDLSAAVLTTTYLNFGHD
                     RDFVFCVETEFRIDAEEELRGHFLLLPDLASLKAIFDAIRVT"
     gene            complement(684..2603)
                     /locus_tag="AC16JC_08570"
     CDS             complement(684..2603)
                     /locus_tag="AC16JC_08570"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HIR5164788.1"
                     /translation="MDLSQYAELFLAESREHLSACNQLLLQWERAPASAEPLGGLFRS
                     VHTVKGMAATMGYARVADLAHRMENLLDRLRKGVAAPSGDMLQLMFRGIDLLEKSIGL
                     AVAGREKDVDVAGLGAELDLAAQARGGSGASAGKATRGRRSGRAASATASLPITDAPV
                     EVSSAGRLVSVALRAEAPLKGGRALLVLRRARALGPVAHVSPPEATFESDDFDGRFSF
                     RLDAAVAAVEIERALVRAGDVERVAVSESDAAVGRGEGAGADRSRHIRVDLRRLDRLM
                     DLIGELVTARGRLNQLAVERRDPAVDDVAIQISRLSSDLQSEIIQARMTPVWQVFDRY
                     PRLVRDLARQLDKQVALRVEGKEIELDRAILDELGDPLVHLLRNAVDHGIESPADRQR
                     RGKAAEGEIVLAAVRERASVTISIADDGRGIDRQRILEKAKRDGIVDAHLESLTDDQL
                     LRVLARPGFSTAEAVTSVSGRGVGIDVAMTKIRALGGSIEVQSEVGRGTTFVLRLPVT
                     LAIVRALIADVGGERYALPLTYVAETVEFGTVPITTVEGREAMVLRNRVVPLVHMRAL
                     LGAAETPQPARRPVVVLQMGERRTGLVMDGMVGPQEIVVKEFDAPAGMLPVFSGATIM
                     GDGVPALILDAGGLV"
     gene            complement(2605..>2897)
                     /locus_tag="AC16JC_08575"
     CDS             complement(2605..>2897)
                     /locus_tag="AC16JC_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013412702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5164789.1"
                     /translation="AGFEVVGEAETGAQAVERYKQLKPDLVTMDIVMPDMGGIDAVRE
                     IVKHDPDAKILMCSAMGQQALVVEAIQAGAKDFVVKPFQPSRVLEAVQRVLG"
BASE COUNT          438 a         1112 c          933 g          414 t
ORIGIN      
        1 ggcgaaggat cggaggacgg cgaggtcgcg atccgagagc gctggcgccg tcacgggtca
       61 ggtgacgcga atcgcgtcga agatcgcctt gagggaggcc aggtccggca gcagcaggaa
      121 atggccccgc agctcctctt cggcgtcgat gcgaaactcc gtctcgacgc agaacacgaa
      181 gtcgcggtcg tggccgaaat tgaggtacgt ggtggtcagc accgcggccg agaggtcgat
      241 gaccaggctg gggaccgacg gcaggaggag catgcccatg aaatccgaca gcgcgttcat
      301 gtaggcgccg gagaggatgt tgcccgcttc cttcagcgcc gactgctcca gcgcgccgaa
      361 tccggtcgtg gcgcccgacg ggcggcgcag cagcatgtcg cacagccgct gccccacctt
      421 ctcgggaaag agcagcaggg tgcgtccggt gaggtcgccc agcatgtgca tcagcaccgc
      481 caccacgacg gcctgcgcgt cgcccagcag atccgcgacc tcctccaggc gcgcgatgtt
      541 gatctgcggc acgctgatca tgatgcggcg gttggtcatc tgcgacaggg cggtggccgc
      601 gtggccggcg ccgatgttgg cgacctcgcg caacgcatcg atctgcagct ccttcaggtc
      661 gcgcacgtcc tccatgccac cccctaaacg agaccgccgg cgtccagaat cagggccggc
      721 accccgtcac ccataatggt cgctccgctg aagacgggca gcatccccgc gggcgcgtcg
      781 aattccttaa ccacgatttc ctgcgggccc accatcccgt ccatcaccag accggtgcgg
      841 cgctcgccca tctggagcac caccacggga cggcgggccg gctgcggcgt ctccgccgcc
      901 ccgagcagcg cgcgcatgtg caccaggggc acgacccggt tgcgcagcac catcgcctcg
      961 cggccttcca cggtggtgat gggcaccgta ccgaactcca ccgtctccgc gacgtaggtc
     1021 agcggcaggg cgtagcgctc gccccccacg tcggcgatga gcgcccgcac gatcgcgagc
     1081 gtgacgggca gccggagcac gaaggtcgtg ccgcgtccga cctcggactg cacctcgatc
     1141 gagcccccca gcgcgcggat cttggtcatc gccacgtcga tgccgacgcc gcgcccggag
     1201 acgctggtca cggcctcggc ggtcgagaac cccggacggg ccagcacgcg cagcagctga
     1261 tcgtcggtga gactctcgag atgggcgtcc acgatgccgt cgcgcttcgc cttctcgaga
     1321 atccgctgcc ggtcgatgcc gcggccgtcg tcggcgatgc tgatggtgac gctggcgcgc
     1381 tcgcgcaccg ccgcgagcac gatctccccc tccgccgcct tgccgcggcg ctgccgatcc
     1441 gccggcgact cgatgccgtg gtccaccgcg ttgcgcagca ggtggacgag cggatcgccc
     1501 agctcgtcca ggatcgcgcg atcgagctcg atctccttcc cctccacgcg cagcgcgacc
     1561 tgcttgtcga gctggcgcgc caggtcgcgc accagccgcg gatagcggtc gaacacctgc
     1621 cacaccggcg tcatgcgggc ctggatgatc tccgactgca gatcgctcga gaggcgggag
     1681 atctggatcg ccacgtcgtc cacggccgga tcgcgccgct cgacggccag ctggttcagc
     1741 cggccccgcg ccgtcaccag ctccccgatc aggtccatga gccggtcgag ccggcgcagg
     1801 tccacgcgga tgtgccggct gcggtcggcg ccggctccct caccccgccc caccgccgcg
     1861 tccgactcgg acacggccac gcgctcgacg tcgcccgccc gcaccagcgc acgctcgatc
     1921 tccaccgccg ccacggccgc atccagccgg aagctgaacc ggccatcgaa atcgtccgac
     1981 tcgaacgtcg cctcgggggg cgacacgtgg gccaccgggc cgagcgcgcg cgcacgccgc
     2041 aacacgagca gtgcccgtcc tcccttgagg ggcgcttccg cgcgcagcgc gaccgacacc
     2101 aggcgtcccg cactcgacac ctcgaccggc gcgtccgtga tcggcaggct tgccgtggcc
     2161 gacgccgccc ggccggatcg ccggccgcgt gtcgccttgc cggccgacgc accgctcccc
     2221 ccccgcgcct gcgccgccag gtccagctcg gcccccaggc ccgcgacgtc cacatccttc
     2281 tcgcgtcccg ccaccgcgag gccgatggat ttttccagga gatcgatacc gcggaacatc
     2341 agctgcagca tgtcgccgct cggcgccgcc acgcccttgc gcagccggtc gagcagattc
     2401 tccatgcggt gcgccaggtc ggccacccgg gcgtagccca tcgtcgccgc catgcccttc
     2461 accgtgtgca ccgaccggaa cagcccgccc agcggctcgg cgctcgccgg cgcccgttcc
     2521 cactgcagca ggagctggtt gcaggcgctc aggtgctccc ggctctccgc caggaacaat
     2581 tcggcgtatt gcgaaagatc catgtcaccc gagcacgcgc tggacggctt ccagcacgcg
     2641 cgacggctga aacggcttca cgacgaagtc cttcgcaccg gcctggatcg cctccaccac
     2701 cagggcctgc tgccccatgg cgctgcacat caggatcttc gcgtcgggat cgtgcttgac
     2761 gatctcccgc accgcgtcga tgccgcccat gtcgggcatg acgatgtcca tcgtgaccag
     2821 gtcgggcttc agctgcttgt agcgctccac cgcctgggca ccggtctccg cctcgcccac
     2881 tacctcgaag ccggcct
//
LOCUS       DBNGZW010000366         1998 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1328305, whole genome shotgun sequence.
ACCESSION   DBNGZW010000366 DBNGZW010000000
VERSION     DBNGZW010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1328305"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1182)
                     /locus_tag="AC16JC_08580"
     CDS             complement(<1..1182)
                     /locus_tag="AC16JC_08580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164790.1"
                     /translation="MTHAAASGDSGRWDSLAVGIALVLAYVVLRGTDLIAIGAFNDDG
                     VYAALGRAIARGDGYRLTYLPGNPVAVKYPPGLPALLAIPWLLGGGIPALRSLIGIVQ
                     PVICGAAAAILWWLARRRLGVSPVVAGVMVVGPFLLDAAIVYYSIPIAEPYLIAGWAT
                     TLLLVDRLARHPPETVATRAAIALGLSLAVTALFRSAGLVLLPAVLISLAFRRRPRRE
                     LAVCAAAALLPLIAWWLLHAHWMSIGPLATLPDERSYLRYVLLDHPVSAAGMAFRSLG
                     SNSVEYALDLVGWLAGRPIPAGLAIAGLAAALVTGAVRQARRRPELVWTVAAYLAAVW
                     LWPFTQDRLLLPVLPFAGLLAADGLESALAARPARWRSAAYAALGLAAAGVGLRQYGL
                     RR"
     gene            complement(1179..>1998)
                     /locus_tag="AC16JC_08585"
     CDS             complement(1179..>1998)
                     /locus_tag="AC16JC_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR5164791.1"
                     /translation="VNMVAAGEAGGILDTILQRLAQFLEKNDAIIRKVKGAMVYPAVI
                     MSVAVIAVTVLLIFVIPTFQNMFASVNLELPLPTRIVIGLSNALKHYWWLLIGVIVLA
                     IFSINRYYKTAAGRLQIDTMLLRFPVIGDVLRKSAVSRFTRTLGTLISSGVSILDGLE
                     ITARTAGNMVIHNAVMESRSSIAGGDTIAAPLQRSKVFPPMVISMIAVGEQTGGLDEM
                     LSKIADFYDEEVDAAVSTMLSLMEPIMIVVLGVIVGGMVVAMYLPIFDMVNAVQ"
BASE COUNT          376 a          672 c          706 g          244 t
ORIGIN      
        1 gcgccgcagg ccgtactgcc ggaggccaac accggccgcg gcgagaccga gcgcggcgta
       61 cgcggcggag cgccagcgcg ccggccgcgc cgcaagtgcc gactccaggc cgtcggccgc
      121 gagcagcccg gcgaagggca ggaccgggag cagcagccgg tcctgcgtga agggccacag
      181 ccacacggcg gccaggtagg cggcgaccgt ccacacgagc tccggcctcc gacgggcctg
      241 ccgaacggca ccggtcacca gcgcggcagc cagccccgcg atcgcgagac cggccggtat
      301 cggccggccc gccagccagc cgaccagatc gagggcgtat tccaccgagt tgctgccaag
      361 cgagcggaag gccatgccag ccgccgagac ggggtgatcg aggagaacgt agcgcaagta
      421 cgatcgttcg tccggcagag tggcaagcgg gccgatcgac atccaatgcg cgtggagcag
      481 ccaccacgcg atgagcggca gcagcgcggc ggcggcgcag acggcgagct cgcggcgggg
      541 acggcggcgg aacgcgaggg agatcagcac cgccgggagc aacaccagtc ccgcgctgcg
      601 aaacaacgcc gttaccgcga gactgagccc cagggcgatc gcggcccggg tcgcgacggt
      661 ttccggagga tgccgggcca ggcggtcgac cagcagcagc gtcgtggccc accccgcgat
      721 caggtacggc tcggcgatgg ggatgctgta atacacgatc gccgcgtcga gcaggaaggg
      781 gccgactacc atcacgccgg cgacgacggg ggacaccccc aaccggcggc gcgcgagcca
      841 ccacaggatg gcggcggccg cgccgcagat caccggctgg acgatgccga tcagcgagcg
      901 gagtgcgggg atgcctccgc ccagcagcca ggggatcgcc agcaatgccg gaaggccggg
      961 aggatacttg acggcgacgg ggtttcccgg caggtaggtg agccgatagc cgtcgccgcg
     1021 ggcgatcgcc cgcccgagag cggcatagac tccgtcgtcg ttgaacgcac cgatggcgat
     1081 caggtcggtg ccccgcaaca ccacatacgc caggaccagc gcgattccga cggcgagcga
     1141 gtcccaccgc cccgagtccc cgctcgccgc ggcgtgtgtc actgcactgc gttcaccatg
     1201 tcgaagatcg gcaggtacat cgcgacgacc atgccgccca cgatcacacc caggacgacg
     1261 atcatgatcg gctccatgag cgacagcatc gtgctcacgg cggcgtcgac ttcctcgtcg
     1321 tagaagtcgg cgatcttgga gagcatttcg tccagtccgc cggtctgctc gcccacggcg
     1381 atcatggaaa tcaccatcgg cgggaacacc ttggagcgtt gcagcggcgc cgcgatggta
     1441 tcgccgcccg caatggacga ccgcgactcc atcaccgcgt tgtggatcac catgttgccg
     1501 gcggtgcggg ccgtgatctc gagcccgtcg aggatcgaga cgccggagga aatcagggtg
     1561 ccgagcgtac gcgtgaaccg cgacaccgcg gatttgcgca gcacgtcccc gatgaccggg
     1621 aagcgcagca gcatggtatc gatctgcagg cggcccgcgg cggtcttgta gtagcggttg
     1681 atggagaaga tggcgaggac gatgaccccg atgagcagcc accagtagtg cttcagcgcg
     1741 ttcgagagcc cgatcacgat gcgggtgggg agcggcagct ccaggttcac cgacgcgaac
     1801 atgttctgga aggtcgggat cacgaagatc agcagcaccg tcacggcgat gaccgccacc
     1861 gacatgatca ccgccgggta caccatggcg cccttcacct tgcggatgat ggcgtcgttc
     1921 ttctcgagga actgggcgag ccgctgcagg atggtgtcga gaataccgcc ggcctcgccg
     1981 gccgccacca tgttgacg
//
LOCUS       DBNGZW010000367         5022 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1534192, whole genome shotgun sequence.
ACCESSION   DBNGZW010000367 DBNGZW010000000
VERSION     DBNGZW010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5022)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5022)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5022
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1534192"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..754)
                     /locus_tag="AC16JC_08590"
     CDS             complement(<1..754)
                     /locus_tag="AC16JC_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012408475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HIR5164792.1"
                     /translation="MTVLRSARQAFGHPGIEPRWTRSAKEAIGTAYAASSRVWFTLSR
                     GMLNEVYYPTVDRPQIRDLQYLVTDGATFLHDERHLACTREYVSAHALGVSLTNEDPE
                     GRYRIEKQVICDPHQSCVLLRTRLVVRPELRDRLRLFALLAPHLEVGGWGNSGFVAEQ
                     SGRVALVAHKGGTWLALGATIPFARCSVGYVGRSDGWTDLADNYALDWEFDAAPDGNI
                     ALTGELDLARGRDFVLGLAFGDTLHNALTTLFQ"
     gene            complement(751..1746)
                     /gene="glk"
                     /locus_tag="AC16JC_08595"
     CDS             complement(751..1746)
                     /gene="glk"
                     /locus_tag="AC16JC_08595"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF014715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HIR5164793.1"
                     /translation="MRVLAGDIGGTNARLAIVVVDGVRVRVEREQRSASREAPSLASI
                     VQAFLAGTDATLEAACFGIPGRVIDGVGRPPNLPWTIDERELAAAIGIARTSIMNDFD
                     AIGHGLALLGAKDVETLQRGEPVAHGPVALIGAGTGLGEAFLTWDAGGYRVHASEGGH
                     VGFAARDDLEWGLRRALQDEFGRVSVERVVSGPGLIAIYRHLVAAGSVAEAGVVREEM
                     GRQDPAAVIVRHGLAGTDPLCAAALDRFAAALGAAAGDLALTVLATGGVYIAGGIAPR
                     IVARLRTGPFLAAFRAKGRLAEFTARVPVHVVVNPDVGLLGAAAVAAHGAGPERS"
     gene            complement(1743..2432)
                     /gene="pgl"
                     /locus_tag="AC16JC_08600"
     CDS             complement(1743..2432)
                     /gene="pgl"
                     /locus_tag="AC16JC_08600"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HIR5164794.1"
                     /translation="MLPDHASFVDAAAGTIAATLARVVEARGRCAVALAGGATPRPVY
                     ACLARAPLVGQVWEHIDLYFTDERCVPSDDPGSNYRMVAETLLSGVPGLAARTHPMQG
                     ERRDRDAAASDYERVLPARLDLMLLGVGPDGHTASLFPRSPALDERVRRVVAVRGPTP
                     PALRLTITPPVIAAAETVVVLVSGAEKAPAVARALAGLYQPREVPSQFARHGDWIMDA
                     EAAATWRSHAA"
     gene            complement(2471..3883)
                     /gene="zwf"
                     /locus_tag="AC16JC_08605"
     CDS             complement(2471..3883)
                     /gene="zwf"
                     /locus_tag="AC16JC_08605"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR5164795.1"
                     /translation="MAGTSTRPTPSDALVLFGVTGDLAHKMIFPALYAMAKRGVLEVP
                     VVGVASSPWSLAQLRARATDAIRKAGRVDDRRALRHLLSRLRYVSGDYNDPRTFATLK
                     RTLAGARRPAHYLAIPPMLFETVIKGLGAAGLADRARVIVEKPFGRDLASARELNGVA
                     RSVFPEDAIFRIDHFLGKEAIMNILYFRFANSFLEPIWNRNYVASVQVTLSEKFGVNG
                     RGAFYETAGCLRDVIQNHLFQIVALLAMEPPPYRGYGAVHNEKAAVFRAMRPLQPADL
                     VRGQYAGYRREPAVSRTSDVETYCALRLFIDSWRWEGVPWYLRSGKLLAENGAEVLVQ
                     LKPPPQRLFADSAPVNGRANYLRFQLSPNSAVALAARVKRAGKEFVGDQGELYLVEEQ
                     PGEEAPYERLLGDAMAGDGALFTREDAVEAAWAVVDRVLKTHSRVHPYKPGSWGPRAA
                     DALIAADGGWHNPAAPHAAP"
     gene            4268..>5022
                     /locus_tag="AC16JC_08610"
     CDS             4268..>5022
                     /locus_tag="AC16JC_08610"
                     /inference="COORDINATES: protein motif:HMM:NF014068.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HIR5164796.1"
                     /translation="MLVGDSPHRILAICSGALLAARAGLVANRRCTTHHEPLQALRAL
                     APRAKVIDNRVFVLDGPLASSAGITAGIDLALHMITEEYGEALATSVAEDMVVSSRCS
                     PCDPELSPFLVHHRHLHAAVHRVQDAIVTRPERDWDMAALAAVAHATERHLSRVFMDH
                     AGVSPLHYLRLIRLERARLAIECGARSRAPPRSRASGRGCSCAGRGNASGAVRREMRR
                     APRRSRDRFGVGPEPAQKAARARPGAHGSCLHCV"
BASE COUNT          754 a         1823 c         1753 g          692 t
ORIGIN      
        1 actggaacag ggtcgtcagc gcgttgtgga gcgtgtcgcc gaaggcaagg ccgagcacga
       61 agtcccggcc gcgcgccagg tcgagctctc ccgtgagcgc gatgttgccg tccggagcgg
      121 catcgaactc ccagtcgagg gcgtaattgt cggcgagatc cgtccacccg tccgagcgcc
      181 ccacgtatcc aacggagcag cgcgcgaacg ggatcgtcgc ccccaaggcg agccacgtcc
      241 cgcccttgtg cgccacgagc gccacgcgac ccgactgctc cgccacgaaa cccgagttgc
      301 cccagccgcc aacctcgagg tgcggcgcca gcagcgcgaa cagcctgagc cggtcccgga
      361 gctcggggcg aaccacgaga cgcgtgcgga gcagcacgca cgattggtgc ggatcgcaga
      421 tcacctgttt ctcgatccgg tagcggccct cggggtcctc gttggtgagc gacaccccga
      481 gcgcgtgagc gctcacgtac tcgcgggtgc acgccagatg ccgctcgtcg tgcaggaacg
      541 tggcgccgtc cgtcaccaga tactggaggt cgcggatctg cggccgatcc acggtggggt
      601 agtagacctc gttcagcatg ccgcgcgaca gcgtgaacca cacgcgactc gatgcggcat
      661 acgccgtgcc gatggcctcc ttggcgctgc gcgtccagcg gggttcgatc cccgggtggc
      721 cgaaggcctg tcgggcgctg cgcagcacgg tcacgaccgc tccggcccgg cgccgtgagc
      781 agcgaccgcc gccgccccga gcaggccgac gtcgggattc acgacgacat ggacgggaac
      841 gcgcgccgtg aactcggcga ggcgcccctt ggcgcgaaac gcggccagga agggccccgt
      901 gcggagccgc gccacgatcc gcggagcgat cccgccggcg atgtacacac cgcccgtcgc
      961 gagcacggtc agggccaggt cccccgctgc cgcgccgagc gccgcggcga accggtcgag
     1021 cgcggcggcg cacagcggat ccgtccccgc caggccgtgc cgcacgatca ccgccgcggg
     1081 atcctgccgg cccatctcct cccgcacgac cccggcttcg gcgacggagc cggcggccac
     1141 gagatggcgg tagatcgcga tcagaccggg ccccgagacg acccgctcga cggagacccg
     1201 cccgaactcg tcctgcagcg cccggcgcag cccccactcg agatcgtcgc gcgccgcaaa
     1261 ccccacgtgt ccgccctccg acgcgtggac gcgatagcct ccggcgtccc acgtgagaaa
     1321 cgcctccccc agtcccgtgc cggcgccgat gagcgcgacc gggccgtggg cgaccggctc
     1381 tccccgctgg agcgtctcga cgtccttcgc cccgagcagg gcgagcccgt gcccgatggc
     1441 gtcgaagtcg ttcatgatcg atgtacgggc gatgccgatc gccgcggcca gctcgcgctc
     1501 gtcgatggtc cacggcaggt tgggcggccg gccgacgccg tcgatcacgc gcccggggat
     1561 cccgaagcac gctgcctcga gcgtcgcatc ggttcccgcg agaaaggcct gcacgatcga
     1621 ggcgaggctc ggagcctccc gggaggcgga gcgctgctcc cgttcgacgc gcacgcgcac
     1681 gccgtcgact acgacgatcg cgagccgcgc gttcgtcccc ccgatgtcgc cggcgagcac
     1741 tctcacgccg catgcgagcg ccaggtggcc gccgcctccg cgtccatgat ccagtctccg
     1801 tgccgcgcga actggctcgg cacctcgcgc ggctgataga ggccggccag ggcgcgtgcc
     1861 acggccggcg ccttctccgc cccgctgacg agcacgacca cggtttcggc cgcagcgatc
     1921 accggcggcg tgatggtgag ccgcagtgcc ggcggcgtcg gcccgcgcac tgcaaccacg
     1981 cggcgaaccc gctcgtcgag ggcgggcgac cgtgggaaca gcgaggccgt atgaccgtct
     2041 ggccccacgc cgagcagcat caggtcgagc cgggccggca acacgcgctc gtagtcggac
     2101 gccgcggcat cgcggtcgcg ccgttccccc tgcattgggt gcgtccgggc cgcgaggccc
     2161 ggcacgcccg acagcagcgt ctcggcgacc atgcgatagt tgctgccggg gtcgtccgag
     2221 ggcacgcacc gctcatccgt gaaatacaga tcgatgtgct cccagacctg gccgaccagc
     2281 ggcgcccggg ccagacacgc gtacactgga cgaggcgttg cgccgcccgc gagggcgacc
     2341 gcgcagcgtc cccgagcctc gacgacccgg gccagggtcg ccgcgatcgt gccggcggcg
     2401 gcatccacga acgacgcgtg atccgggagc acgactcgct caccgtgcgc ggctgctcca
     2461 gacggcgccg tcacggggcc gcgtgcggcg cggcggggtt gtgccagccg ccgtccgccg
     2521 cgatgagcgc gtccgccgcc ctgggacccc agctgccggg cttgtatgga tgaacccggg
     2581 aatgcgtctt gagaacgcga tcgaccacgg cccaggcggc ctccaccgcg tcttcccgag
     2641 tgaacagggc gccgtcgccg gccatcgcat cgcccagcag ccgttcgtac ggcgcctcct
     2701 ctcccggctg ctcttcgacc agatagagct ctccctggtc gcccacgaac tccttcccgg
     2761 cgcgcttgac gcgggcggcg agggccaccg ccgagttggg cgaaagctga aagcgcagat
     2821 agttggcgcg accgttcacc ggcgcggagt ccgcgaagag acgctgcggc ggcggtttga
     2881 gctggaccag cacctcggcg ccattctcgg ccagcaactt cccggagcgc aggtaccagg
     2941 gcacgccctc ccagcgccag gagtcgatga acaggcgcaa cgcgcagtac gtctcgacgt
     3001 ccgaggtgcg tgacacggcc ggctcccggc ggtagcccgc gtactggccg cgcaccaggt
     3061 cggcgggttg cagcgggcgc atggcccgga agacggccgc tttctcgttg tgcacggcgc
     3121 cgtacccccg gtagggcggc ggctccatgg cgagcagcgc gacgatctgg aacaggtggt
     3181 tctggatcac gtcgcgcagg cagccggcgg tctcgtagaa tgcgccgcgt ccgttcactc
     3241 cgaatttctc ggagagggtc acctggacgc tggccacata attgcggttc cagatgggct
     3301 ccagaaacga gttggcgaag cggaagtaca ggatgttcat gatcgcttcc ttcccgagga
     3361 agtgatcgat gcgaaagatc gcatcctccg ggaagaccga ccgcgcgacg ccgttgagct
     3421 cccgcgccga ggccaggtcg cgaccgaacg gtttttcgac gatgacccgt gcccggtcgg
     3481 ccaacccggc ggcgccgagc cccttgatca ccgtttcgaa caacatgggc ggaatggcga
     3541 ggtagtgcgc cgggcgccga gcccctgcca gggtccgctt gagcgtcgcg aacgttcgcg
     3601 ggtcgttgta gtcgccgctc acgtagcgga gtcgggagag gaggtggcgc agggcgcgcc
     3661 ggtcgtcgac ccggccggcc tttcgtatcg cgtccgtggc acgggcgcgg agctgcgcca
     3721 gactccacgg tgaggacgcg acgccgacca ccgggacctc gagcacgccg cgcttcgcca
     3781 tcgcgtacag ggccgggaag atcatcttgt gggcgagatc gcccgtcacg ccgaacagca
     3841 ccagcgcatc ggacggcgtc gggcgggtgg aggtgccggc catgtcagcc gcctccctgc
     3901 tgcttctcca cgtgcccgcg gaactgcttg cgcatggcgg agagcacgcg gtcggcgtag
     3961 gcacgcatac tgcccactcc gatcatgccg aattgcatcc cgaacctcct tgcctcacgc
     4021 gctggggacc aatccgacgc ctgggcggat acgtgagaac catgccgtcc acctgcgcag
     4081 gagggtgcga cgcgcagact ggcgagggcg gggcgtcttc ggggcgacag gatattaccc
     4141 caccgcctcc gcaaccgcga ggagcggcca ggatgcgccg cttcggttca ttcccaatct
     4201 tcggacatct cgagcccggc gactcggtgt gggtctgcgt acgcacgggc gatgtcgcgt
     4261 agcgctcgtg ctcgtcggtg acagcccgca tcgtatactc gcgatctgct caggggcgtt
     4321 gctcgcggcg cgcgcgggac tggtcgcaaa tcggcgctgc accacgcacc acgagccgct
     4381 ccaggcgctg cgggccctgg cgccccgagc gaaggtgatc gacaaccgcg tgttcgtgct
     4441 ggacggaccg ctggcgtcga gcgcgggcat caccgcgggc atcgacctcg cgctgcatat
     4501 gatcacggag gaatacggcg aagcgctcgc aaccagcgtc gccgaggaca tggtggtctc
     4561 cagccggtgc tcgccgtgcg accccgagtt gtcgccgttc ctggtgcacc accgccatct
     4621 gcacgccgcg gtccaccgcg tgcaggatgc catcgtcacg cgtcccgagc gcgactggga
     4681 catggccgcg ctggcggcgg tcgcgcatgc caccgagcgc catctgtcgc gggtgttcat
     4741 ggaccacgcc ggcgtctcgc cgctgcacta cctgcggctg atccgcctcg agcgcgcgcg
     4801 cctcgcgatc gagtgcggtg ccaggtcacg cgcgccgccg aggtcgcggg cttccggtcg
     4861 gggctgcagc tgcgccgggc gtggaaacgc cagtggggcg gttcgccgcg agatgcggcg
     4921 cgcgccgcgg cgttctcgtg accgtttcgg tgtcggaccc gaaccggccc aaaaagcggc
     4981 gcgcgcccga ccgggcgcgc acggtagctg tttgcactgc gt
//
LOCUS       DBNGZW010000368         3346 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1260250, whole genome shotgun sequence.
ACCESSION   DBNGZW010000368 DBNGZW010000000
VERSION     DBNGZW010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3346)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3346)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3346
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1260250"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..2938)
                     /locus_tag="AC16JC_08615"
     CDS             complement(44..2938)
                     /locus_tag="AC16JC_08615"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR5164797.1"
                     /translation="MKAAVNPDQEREVLSAALRDLHGSAAVLESWAADPLSKRGRNRV
                     VRYDVRARVPGTSDIRSHLWVGKFYEREDDARTIAAILEQLGGTDSGHGDGLLIPRVL
                     GFYPALRMVLQDYEAGESVISAMGRYNGEVVGAIGRALATLHRSDVRLAARTTPADVL
                     ERLRPKLVELSARYPERAFALRHALVTLENQVPAMPEPRFLHGDLGAAQMLWATGRIV
                     LLDLDNCTRGDPALDLGALLAQLRRLSLRKPAKVPDLAVMRAGLLAAYERAAPADLGL
                     AQRVAWYEVAALLRKIHFLVFDTTRHAEPEAIEQRRAEAERLMGQLPPHALGTFPQLA
                     ALSEARASRNGAARPREKPSISRRAEQRERRKRLRRFFGTQLGRVKWRLLVAGVCTLG
                     TSLTDLLQPWPLKLILDHGLLEKPLPDALWFLEPVVEQAGGRFLVLASFAMVMIAILG
                     AVFSYFQKFITSDISFRTVYALRRELFVHMQRLPLSFHTGARAGDLLNRVQSDTDTLK
                     DMVSDDLLKFCSQILSVIGMCVILMLINLDLALIALATLPLLSFSLFHLYRKSKVSAK
                     KQKQQDGKVAARMVEVLSAIPLVQAFGREGHEEERFDAVTSVSLRESIRVARLSAAAS
                     RSSEIITAAGTAAAVLYGAFQVQSGNMLPGVLVLVVAYLNNFYKPLRGLAKLSTDFSK
                     SMASADRLSEVLDIEPRIRDRADAVEAPRFKGEIAFDKVCFAYDEVEDDVLQDLSFRV
                     APGQRVALVGVSGAGKSTIGNLILRLYEPRRGAILVDGVDVQRYTRDSLRRQIGVVLQ
                     SSILFGSSVKENIAYGTPEATEEQIEAAARAANADEFIRELPQGYDTVIGERGATLSG
                     GQQQRIAIARALIRDAPILILDEPMTGLDVESEAKVREALDRLMTGKTCIMMTHDLQA
                     IADAHQVLVLEGGQLVAQGAHADLLATSARYRELYEMDRDQAQAVEEPSA"
BASE COUNT          542 a         1172 c         1119 g          513 t
ORIGIN      
        1 ggctccggca gcggctgctt gcgcccgatg gatcgcccgc cggtcatgcg ctcggctcct
       61 cgaccgcctg ggcctgatcc cgatccatct cgtacagctc gcggtagcgg gcgctagtgg
      121 ccaggaggtc ggcatgcgcg ccctgagcga cgagctggcc gccctccagg accagcacct
      181 gatgggcgtc ggcgatcgcc tgcaggtcgt gggtcatcat gatacaggtc ttcccggtca
      241 tcagacgatc cagtgcttcg cgcaccttgg cttcactctc cacgtcgagg cccgtcatcg
      301 gctcgtccag aatcagaatg ggggcgtcgc ggatcagcgc gcgggcgatg gcgatgcgct
      361 gctgctggcc gcccgaaagg gtcgcgcccc gctccccgat cacggtatcg tagccctggg
      421 gcagctcgcg gatgaactcg tcggcgttgg cggcacgggc cgccgcttcg atctgctcct
      481 cggtcgcctc cggggtgccg tacgcgatgt tctccttcac cgaggagccg aacagaatcg
      541 agctctgcag cacgacgccg atctggcgcc gcagcgaatc gcgggtgtag cgctgcacat
      601 cgaccccgtc gacgagaatc gccccccgcc ggggctcgta caaccgcagg atcaggttcc
      661 cgatggtgga tttccccgcg ccggacacac cgaccagcgc cactcgttgt ccgggcgcga
      721 cgcggaagga caggtcctgg agcacgtcat cctcgacttc gtcataggcg aagcagacct
      781 tgtcgaaggc gatttctccc ttgaagcgcg gtgcctcgac ggcatcggcg cggtcccgga
      841 tccggggctc gatgtccagg acttcggaga gccggtccgc gctggccatg gatttggaga
      901 agtcggtcga cagcttggcc aggccgcgca gcggcttgta gaagttgttg aggtaggcaa
      961 cgacgagcac gagcaccccc ggcagcatgt tgccgctctg gacctggaac gcgccataca
     1021 gcaccgccgc cgccgtgccc gccgcggtga tgatctccga cgaccggctc gcggccgccg
     1081 acagccgcgc gacccggatg ctctcgcgca gcgacaccga cgtcacggcg tcgaaccgct
     1141 cttcctcgtg cccttcgcgc ccgaacgcct gcaccagcgg gatcgccgac agcacctcga
     1201 ccatccgggc cgcgaccttg ccgtcctgct gcttctgttt cttcgccgag actttgctct
     1261 tgcgatacag gtggaacagg ctgaacgaga gcagcggcag cgtggccagg gcgatgaggg
     1321 ccagatccag gttgatgagc atcagaatga cgcacatccc gatgaccgac aggatctgcg
     1381 aacagaattt cagcagatcg tcggagacca tgtctttcag cgtgtccgta tccgactgga
     1441 cccgattcag caggtcgccc gcccgggcgc cggtatggaa cgagagcggc aggcgttgca
     1501 tgtgcacgaa cagctcccgg cgcaacgcgt acaccgtccg gaagctgatg tcagaggtga
     1561 tgaacttctg gaagtaggag aacaccgcgc ccaggatcgc gatcatcacc atcgcgaacg
     1621 atgccagcac caggaaccgg cccccggcct gctccaccac aggctcgagg aaccagaggg
     1681 catcgggcag gggtttctcc agcagcccgt gatccaggat gagcttcagg ggccacggtt
     1741 gcagcaggtc ggtgagcgac gtgcccaggg tgcacacgcc cgccaccagc agccgccact
     1801 tgacccgccc caactgggtg ccgaagaacc gccgcaggcg cttgcggcgc tcgcgctgtt
     1861 cggcgcgccg gctgatcgag ggtttctcgc gcggccgtgc agccccgttg cggctcgcac
     1921 gcgcttcgct cagtgcggcc agctgcggaa aggtccccaa ggcatgcggc ggcagctggc
     1981 ccatcaaccg ctccgcctcg gcccggcgtt gctcgatcgc ctccggctcg gcgtggcgcg
     2041 tggtgtcgaa caccagaaag tggatcttgc gcagcagcgc ggccacttca taccaggcca
     2101 cgcgctgcgc caggccgagg tcggcagggg cggcgcgctc gtaggcggcg agcaggccgg
     2161 cacgcatcac ggcgagatcc gggacctttg cgggcttgcg cagggacagg cggcgcagct
     2221 gcgccagcaa cgcacccaga tccagtgccg ggtcaccccg cgtgcagttg tcgagatcca
     2281 gcaggacgat gcggcccgtg gcccagagca tctgggcggc cccgagatcg ccgtgcagga
     2341 aacgcggctc gggcatcgcc ggcacctgat tctcgagggt gaccagggcg tgtcgcaggg
     2401 cgaaggcccg ctcggggtac cgcgccgaga gctccaccag tttcggccgc aaccgctcga
     2461 gcacgtccgc cggcgtcgtc cgggccgcga gccggacgtc gctgcgatgc agcgtcgcca
     2521 acgcccgacc gatggccccc accacctccc cgttgtagcg ccccatggcc gaaatcaccg
     2581 actcgccggc ttcatagtcc tgcagcacca tgcggagggc cgggtagaag ccgaggacgc
     2641 gcgggatgag caggccgtcg ccgtgccccg aatcggtgcc tcccagctgc tcgaggatcg
     2701 cggcgatggt tcgggcgtcg tcctcccgct cgtagaactt ccccacccag aggtggctcc
     2761 ggatgtccga agtgcccggc acgcgggcgc gaacgtcgta gcgtaccacc cggttgcggc
     2821 cccgtttgct gagcggatcg gcggcccaac tttcgagcac ggccgcggac ccgtgcaggt
     2881 cgcgcagcgc ggcgctcagg acctcacgct cttgatcggg attgactgcg gccttcacgg
     2941 cagcacggcg ggcgatggga ctccgggcgt acggggagcc cgatggctcg cagccgttcc
     3001 agagggctcc ggggccgtgc aggaccaccc caatcgaagt ctcgcgttca ttcgtccgtt
     3061 cctggagggc aaaaccggtt atccgccgga ggcggtgaag ggcagtcccc ctcgctggtc
     3121 caacacgcag cgcccgtgcc gccgctcggg ccccccggat cgagcacgcg ggcgttgtga
     3181 cgcaccggtt tgcaaccggg gtcgccccga atgcgcgcac cagtatgcaa cggcagcctg
     3241 tggggcagcg ccccaacttg cagcatgggt ccgctcgctg gcgcgaacac gtccgccaag
     3301 cctcacggat tccttgtcgt gcaaggccgg cccgttcgac aacgtc
//
LOCUS       DBNGZW010000369         3367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1796757, whole genome shotgun sequence.
ACCESSION   DBNGZW010000369 DBNGZW010000000
VERSION     DBNGZW010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1796757"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..241)
                     /locus_tag="AC16JC_08620"
     CDS             complement(<1..241)
                     /locus_tag="AC16JC_08620"
                     /inference="COORDINATES: protein motif:HMM:NF021505.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HIR5164798.1"
                     /translation="MIPNPLHPSLVHFPIVFAVLLPLVAIGALWMIQRGAAARRAWLL
                     PVALAGALTLSAFLAVQTGKAQEERVEDVVGRQPLH"
     gene            361..927
                     /locus_tag="AC16JC_08625"
     CDS             361..927
                     /locus_tag="AC16JC_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5164799.1"
                     /translation="MDEGIDRRLRLVLALVLLGIAAAAGIDLALDAPRSWVSFHAIYE
                     LVVVAGALVTATALWLGWVRAVRSVAAMRRSLAERGAEADAWRARAQRVLAGLSRAID
                     EQFSTWKLTPTEREIALELLKGRSHKEIAADTGRSERTVRQHAVVVYQKAGLSGRAEL
                     GAFFLEGLMLPSVSDPDVVRPPAVPESS"
     gene            complement(878..1663)
                     /locus_tag="AC16JC_08630"
     CDS             complement(878..1663)
                     /locus_tag="AC16JC_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164800.1"
                     /translation="MKRSSLALGLLVLLGARAIRAQDNYEIQVYGSELVPVGATMLEL
                     HSNYTFDGQPGTAGVRTSRHALHETVEITHGFSEWLEVGFYLFTSTKAGTGLEWVGDH
                     VRPRLSAPARWHWPVGVSLSQEIGYTRRSYSEDTWSWEIRPIVDRHFGPLYVSLNPVL
                     DRALSGPGAGAGFELSPNAQAALDVSPRVNLALEYYGGFGPVNDPAGLNQSSQQIFPA
                     VNLDFGDDWEFNLGIGVGLTPATDRLLVKMILGRRVGGRRPDH"
     gene            1710..1943
                     /locus_tag="AC16JC_08635"
     CDS             1710..1943
                     /locus_tag="AC16JC_08635"
                     /inference="COORDINATES: protein motif:HMM:NF015956.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HIR5164801.1"
                     /translation="MTHTLADLPFGRDAVLAELAVPADDAARLMELGFLPGSRVRAVR
                     AAPGGDPRVFRVDGAEIALRRETARLLRLLPEP"
     gene            1940..>3367
                     /gene="feoB"
                     /locus_tag="AC16JC_08640"
     CDS             1940..>3367
                     /gene="feoB"
                     /locus_tag="AC16JC_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HIR5164802.1"
                     /translation="MSGCHDCVLAAHVDPGTSPGVVSVALVGPPNSGKSSLFNRLTGL
                     RQKVANYPGVTVEQRRGRVRLGAGRDVQLLDLPGTYALQSRSEDERITDDVLHGRMPG
                     IPRPDAILLVLDSTNLARHLVLAAPVLSLGLPTLVVLNLTDDLERRGGSLDPAALAEQ
                     LGAPVTLASAATGRGLDTIRQFLAGRFAVPARIPLPVVQNIPACRLWAGRLASQSTYR
                     APAPPLWTRRLDGVFLHPVVGPLLLLTVVLGVFQAMFAAAAPLSDGLEAVVGVAGQWA
                     GALLPAGVVRDLVVQGVLAGVGSVIVFLPQIAVLFLFIGILEDSGYLARAALIADRTM
                     ARIGLQGKSFIPLLSAYACAVPAILATRTIESKRDRLATILVAPFMTCAARLPVYALI
                     IAAFLPNTPLLGPVFGVRAAALLGLYVLGFLAAVGTALLLRSTILKGDRAPFLLELPP
                     YRWPTLRSLGLRLVDRSRAFLTRAGT"
BASE COUNT          437 a         1201 c         1178 g          551 t
ORIGIN      
        1 agtgcagcgg ttgtcggccg acgacgtcct cgacgcgttc ttcctgggcc ttgccggtct
       61 gcacggcgag gaacgcggac agcgtcaggg cgccggccag ggcgacgggg agcagccagg
      121 cgcggcgggc ggcggcgcct cgttggatca tccacagcgc gccgatcgcc acgagcggca
      181 gcagcaccgc gaacacgatc gggaaatgca ccagcgacgg gtgcagcggg ttgggaatca
      241 tacgggtatc ctctgtctgg ttgagcgcgc aacatggagc gtgcggccgc gccgcggtat
      301 ccgacgttcg acagcctcgc gcggcaattt cgcgcctcca gcaaaagaca gagggctcgt
      361 atggatgaag gaatcgacag acgcctgcgg ctcgtgctgg cgttggtgct gctgggtatc
      421 gcggcggcgg cggggatcga cctggcgctg gacgcccccc gcagctgggt gtcgttccac
      481 gcgatttacg agctggttgt cgtcgcgggc gcattggtca cggcgacggc gttatggctc
      541 gggtgggtcc gggcggtgcg ctcggtggca gcgatgcgtc gctcgctggc ggaacgcggg
      601 gccgaggccg atgcatggcg ggcgcgggcg caacgcgtgc tggcgggtct gtcgcgcgcg
      661 atcgacgagc agttcagcac ctggaagctc accccgaccg agcgggaaat cgcgctggag
      721 ctcttgaagg ggcggagcca caaggaaatc gcggcggaca ccggccgcag cgagcgcacc
      781 gtgcgccagc atgccgtggt cgtctatcag aaggcggggt tgtcggggcg ggcggagctc
      841 ggcgcgttct tcctggaggg cctgatgctg ccgtccgtca gtgatccgga cgtcgtccgc
      901 ccacccgccg tcccagaatc atcttgacga gcagccggtc ggtcgccggc gtgagcccta
      961 caccgatgcc gaggttgaac tcccagtcgt ccccgaagtc gaggttcacc gcgggaaaga
     1021 tctgctgtga gctctgattc agtccggccg gatcgttcac ggggccgaat ccgccgtagt
     1081 actcgagcgc caggttcacg cggggtgaga catcgagcgc cgcctgggcg ttgggggaga
     1141 gctcgaatcc cgcgccggca ccgggtcccg agagagcgcg atcgagcacc gggttgagcg
     1201 agacgtacag gggcccgaag tgccggtcca cgatcggccg gatctcccag ctccaggtgt
     1261 cctccgagta cgaccgtcgc gtgtagccga tttcctggga caggctcacc cccaccggcc
     1321 agtgccagcg cgcgggcgcg ctgagccgcg gtcgcacgtg atctccgacc cactccaggc
     1381 ccgtcccggc cttggtgctg gtgaagaggt agaagcccac ttcgagccac tcgctgaacc
     1441 cgtgggtgat ctcgaccgtc tcgtgcagtg cgtgacggct ggtgcgcacg ccggccgttc
     1501 ccggctggcc gtcgaaggtg tagttgctgt gcaactccag catcgtcgcg ccgaccggca
     1561 ccagctccga gccgtagacc tggatctcgt agttgtcctg ggcccggatg gcgcgcgccc
     1621 cgaggaggac gagcaggccc agcgcgagtg aagacctctt cacgcgggct tcgtgaagcc
     1681 ttgagcccat cctcactttg tttagacccg tgacccacac gctggcagac ctcccgttcg
     1741 gtcgtgacgc cgtgctggcc gagctcgcgg tcccggccga cgacgcggcg cggctcatgg
     1801 agctgggctt tctcccgggc tcccgggtgc gcgcggtgcg cgccgcgccc ggtggcgacc
     1861 cgcgcgtgtt ccgcgtggac ggcgccgaaa tcgccctgcg gcgcgaaacg gcgcgcctgc
     1921 tgcgcctgct gcccgagccg tgagcggctg ccacgactgc gtactcgcgg cgcacgtcga
     1981 tcccggaacg tctcccgggg tcgtgagcgt cgcgctcgtc ggaccgccca actccggaaa
     2041 atcgtcgctc ttcaaccggc tcaccggatt gcgccagaag gtcgcgaact accccggcgt
     2101 caccgtggag cagcggcgcg gccgggtgcg gctcggcgcc gggcgcgacg tccagctgct
     2161 cgaccttccc ggcacctacg cgctgcaatc acggtccgag gacgagcgca tcaccgacga
     2221 cgtgctgcac ggccggatgc cgggcatccc gcggcccgac gcgatcctcc tggtgctcga
     2281 ctcgaccaac ctggcgcgcc acctcgtgct ggcggcgccc gtcctgtcgc tcggcctccc
     2341 cacgctcgtg gtcctcaacc tgacggacga cctcgagcgg cgcggcggct cgctcgatcc
     2401 cgccgcgctg gccgagcagt tgggtgcgcc ggtcacgctg gcgagcgccg ccaccgggcg
     2461 gggactcgac acgatacgcc aattcctggc gggccgcttc gccgtccccg cccgcatccc
     2521 gctgcccgtg gtccagaata tccccgcctg ccggttgtgg gcgggacgac tcgcgtccca
     2581 gtccacctac cgggccccgg cgccgccgct ctggacgcgc cgtctcgacg gcgtgttcct
     2641 ccatccggtg gtggggccgc tgctgctcct gacggtcgtg ctcggcgtct tccaggcgat
     2701 gttcgccgct gccgcgcccc tctcggacgg gctcgaagcg gttgtcggcg tcgcgggtca
     2761 gtgggccggc gccctgctcc cggccggcgt agttcgtgac ctggtggtgc agggagtcct
     2821 ggccggcgtc gggtcggtga tcgtgttcct gccgcagatc gccgtgctct tcctctttat
     2881 agggatcctg gaggactcgg ggtacctggc gcgggcggcg ctcatcgccg accgcaccat
     2941 ggcgcgcatc gggctgcagg ggaaatcctt cattccgctg ctctccgcct atgcctgcgc
     3001 ggtgccggcg atcctcgcca cccgcaccat cgagagcaaa cgcgaccggc tcgccacgat
     3061 cctcgtcgcc cccttcatga catgcgctgc gcgactgccg gtctacgccc tcatcatcgc
     3121 ggccttcctg cccaacaccc cgctgctcgg tcccgtgttc ggggttcgcg ccgccgcgct
     3181 gctcggcctc tacgtcctcg ggttcctcgc cgcggtgggc acggcgctgc tgctccggtc
     3241 cacgatcctc aagggcgacc gcgcgccgtt cctgctcgag ctgcccccct accgctggcc
     3301 cacgctgcgc tcgctcgggc tgcggctggt ggatcgctcg cgcgcgtttc tcacccgcgc
     3361 cgggacg
//
LOCUS       DBNGZW010000370         2292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1466010, whole genome shotgun sequence.
ACCESSION   DBNGZW010000370 DBNGZW010000000
VERSION     DBNGZW010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1466010"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1881)
                     /locus_tag="AC16JC_08645"
     CDS             complement(<1..1881)
                     /locus_tag="AC16JC_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR5164803.1"
                     /translation="MRVGIDVGGTFTDLVAVTPDGSLEIRKVLSTPADPSEGLFRALD
                     VLATDGAPIETLIHGTTIATNALLERRGARTVLVTTAGFEDLLWLRRQDRASLYDLSR
                     DHPPPLVRRDDVVGVAERMGPEGVVTALSDAEVARVVVAVRDRKAEAVAITFLFAFRY
                     PEHERRIAAAVRAALPGIPVVASHEVVPLFREYERCATTTAEAYLRPLVTEAVTRAAA
                     GVARLGIGTFRLMASGGGTLSAEGAANAAAALALSGPVGGVVGAQLLGEALGHRDLLT
                     LDMGGTSADASLIQGGVASREGSTVVGGVPLALPAVLIETVSAGGGSIAWVDDAGTLK
                     VGPRSAGAQPGPACFGRGGMEPTVTDACLALGWLDPDWTLADAVQLDAAAAEQALRRL
                     RKALGMDVRVVAAGVVAVATAVMARALKRVSVARGEDPRGIALVPFGGAGPLFGCALA
                     DLLGMRTVIVPPHPGVLSALGLAAAAERIEMAASLHQRAAAVQREFVLSAYAELEARA
                     TRALPGAVLRRLADCRFAGQGYEITVPLTDGEPDSIEAAFHAAHRARYGHLGAAGAVE
                     IVNVRVVAERAGGRPVWGRRAGIGRPTPGSRRIVVGGKAIRAQVWPLGELPPRLRIPG
                     PAI"
     gene            complement(1997..>2292)
                     /locus_tag="AC16JC_08650"
     CDS             complement(1997..>2292)
                     /locus_tag="AC16JC_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164804.1"
                     /translation="GRTAGPYQHASHLVLRAIETRMGIARAANDGVSELVDPLGRISN
                     ASRLRTRTVVTGTLLTSDVRTLYVRLGDWVGLLVVLATIGFAGGIVVQKWSRG"
BASE COUNT          357 a          879 c          766 g          290 t
ORIGIN      
        1 gatcgcgggt ccgggaattc gcagccgcgg gggcagctcc cccaacggcc agacctgcgc
       61 ccggatcgcc ttaccgccga ccacgatgcg ccgcgacccc ggcgtcggcc ggccgatccc
      121 cgcgcgacgc ccccagacgg gccggccacc cgcccgctcc gccacgacgc gcacgttgac
      181 gatctcgacc gctccagcgg ccccgaggtg accgtagcgc gcccggtgcg cggcatggaa
      241 cgccgcttcg atgctgtcgg gctcgccgtc cgtcaacggc acggtgatct catagccctg
      301 ccctgcgaag cggcagtccg ccaggcgacg cagcaccgcg cccggcagcg cccgggtagc
      361 gcgggcctcg agctccgcgt atgcggagag cacgaactct cgctgcaccg ccgcggctcg
      421 ctggtgcagg gaggcggcca tctcgatccg ttccgcggcg gcggcgagcc ccaacgccga
      481 gagcacgccc gggtgagggg gcacgatcac ggtccgcatc cccagcaggt ccgcgagcgc
      541 gcatccgaag agcgggcccg caccgccgaa cgggacgagc gcgatgccgc gcggatcctc
      601 gccccgcgcg acactcaccc gcttcagggc gcgggccatg acggcagtgg ccacggccac
      661 gacgcccgcc gcgacgacgc gcacgtccat ccccagcgcc ttcctaagtc gcctcagggc
      721 ctgctcggcg gcggccgcgt cgagctgcac cgcatcggcc agcgtccaat cggggtcgag
      781 ccagccgagc gccagacagg catcggtgac tgtcggctcc atcccaccgc ggccgaaaca
      841 cgccggcccc ggctgggcgc cggcgctccg cgggcccact ttgagcgtcc ccgcgtcgtc
      901 cacccaggcg atgctccctc cgcccgcgct cactgtttcg atgagcacgg cgggcagcgc
      961 gagcgggacg ccgccgacca ccgtggaccc ttcccggctc gccacgccgc cctggatcag
     1021 gctggcatcg gcgctcgtgc ctcccatatc gagcgtcaac aggtcacgat ggcccaatgc
     1081 ctccccgagc agctgcgcgc cgacgacgcc cccgacgggg cccgacagcg ccaatgcggc
     1141 ggcggcattc gcggcgcctt cggccgagag ggttcctccg ccggacgcca tgagccggaa
     1201 cgtgccgata ccgagacggg ccacgccggc cgcagcccgg gtgacggcct ccgtcaccag
     1261 cggccggaga taggcttcgg ccgtggtggt cgcacaccgc tcgtactccc ggaacagcgg
     1321 caccacctcg tgactggcga cgaccgggat cccgggcagt gcggcacgca ctgccgctgc
     1381 gatgcggcgc tcatgttcgg gatagcgaaa ggcaaacagg aaggtgatgg cgacggcttc
     1441 ggccttccga tcgcggactg ccacgacgac gcgagccacc tcggcgtcgg agagcgccgt
     1501 caccacgccc tcaggaccca tccgctcggc gacgccgacc acgtcgtcgc gacgcaccaa
     1561 gggagggggg tgatcgcggc tcaggtcgta gagggaggcg cggtcctggc gccgcagcca
     1621 cagcaggtcc tcgaagcccg ccgtcgtcac caggaccgtc cgggcgccgc gccgctccag
     1681 caatgcattg gtcgcgatgg tggtcccgtg gatcaacgtc tcgatcggcg caccgtcggt
     1741 cgccaggacg tcgagggccc ggaacaatcc ttcggaagga tcggccggcg tgctcaggac
     1801 cttgcggatt tcgagactgc cgtcgggggt gacggccacg agatcggtga atgtgccgcc
     1861 gacgtcgatc cctacccgca cccttccctc ccattgcgga ttggggattg cggattgcgg
     1921 atcggacagg gcgtgtccgg cacgacgcct cgacacgcat caatccgcaa tccacaatcc
     1981 acaatccgca atctcatcag ccccggctcc atttctgcac cacgatgcca cccgcgaacc
     2041 cgatcgtcgc cagcacgacc agcagcccca cccaatcacc caggcggaca tagagcgtcc
     2101 gtacgtcgct ggtcagcagc gtgccggtga cgacggtgcg cgtccggagt ctgctcgcat
     2161 tcgagatccg tcccagcgga tccacgagct ccgagacgcc gtcgttggcg gcgcgagcga
     2221 tccccatgcg ggtctcgatg gcgcgcagca cgaggtgtga ggcgtgctgg tagggccccg
     2281 cagtccggcc ga
//
LOCUS       DBNGZW010000371        16815 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_513316, whole genome shotgun sequence.
ACCESSION   DBNGZW010000371 DBNGZW010000000
VERSION     DBNGZW010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 16815)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16815)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16815
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_513316"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2004)
                     /locus_tag="AC16JC_08655"
     CDS             complement(<1..2004)
                     /locus_tag="AC16JC_08655"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HIR5164805.1"
                     /translation="MLALLLALQTSSDPGPVYRGSARELDVVPPRIEAAARIDGVLDE
                     APWSRAAVLTGFSQYRPSDGRPAEDSTAVLVWYAPDAIYFGIRAFERHGGALVRATLA
                     NRDNIDADDRVQLLLDTYADHRRALLFAVNPLGVQEDGVWSDGVEASAGGPQAGGRFD
                     ATIDLNPDFVFESRGRVTPTGYEVEVRIPFKSLHYQSADPQTWGIQVDRVIQHSGYED
                     TWTPAVRASASFLIQSGRLTGLTGLHRGLVMDITPEVTTKVNGAPGATAYGYTTTPDA
                     GGTLRWGVTENLSLTAAANPDFSQVEADVGQVTANARFPLFFPEKRPFFLEGLEQYDS
                     PNRLIYTRRVAEPLGGAKLTGKIGSTNVAYLAAVDDRDQSFGRSNPVYNLLRLRRDLG
                     ASSTAGLVYTDRVDGSAYNRVAGTDLRVVWKKLWFTQAQVVGSWTRDPAGGGARAGTL
                     WDLTAFDRTGRAYGNHGEIQGVSPDFVAGSGFVPRTDYVSIRLFNRFSWYGRPGALVE
                     QVSTFVGFDPLWHYDDFLHLRSTIEGGFSDSWNASLRGGWGLSVNVSDNQQRFDRADY
                     LGYATDSAGTPFVTPHGLYNLLGGNVGLNTPNRPLTLSVNVGYGAAPIFAEAAEGREL
                     DMNGTVTWRPTQSVRIEALWTHQRITRARDGSRFSTANIPRLKL"
     gene            complement(2128..3339)
                     /locus_tag="AC16JC_08660"
     CDS             complement(2128..3339)
                     /locus_tag="AC16JC_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5164806.1"
                     /translation="MLIDETIRVALQAIRANKLRSLLTALGIIIGVGAVITMIALGTG
                     ARKAIEARIQALGPTLLTLFAGQSFRGGIAMNTRVSLTMDDDTALAHNARYVSAVVPE
                     LASNMQVQLGNQNVNVNVVGTIPAYAKVHAYELTAGRMFTTGEDEARRRFAVLGSAVP
                     DMFNANAAAMIGRDIQIRGIPFTVIGILSEKGSQGFQNPDEQVLIPLQTARYRIMGTD
                     RLRSITVQVDSVKNMNLAMIEVERIMRRQHKIRAGGENDFQIRNQADVLSTFQQTTET
                     FGFLLAGIAAVSLLVGGIGIMNIMLVSVTERTREIGVRKALGATKGNILLQFLVEALV
                     LCLVGGTVGVLFGSLGAVVLSKVAHWNTAISPFSIFLAFAFSALVGLFFGIYPARRAA
                     SLDPIIALRYE"
     gene            complement(3342..4901)
                     /locus_tag="AC16JC_08665"
     CDS             complement(3342..4901)
                     /locus_tag="AC16JC_08665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5164807.1"
                     /translation="MKRLLLTAIAAAGWTACKKPEPTPVYEKVAVERRDIVVTASAAG
                     AIQPLLTVDVKSQASGAITEMRAQTGDDVRPGQLLALVDPRLPKSALDQARANLAVAR
                     AQFENASTQLAREDTLLKTQAITQAEYETAKVAYAQAQAGLVNAQSNLADAQIAWDQT
                     RVTAPLNGTVIQKNVELGTVISSPTRDVGGGTVLFRMANLDTVQVQALVDETDIGKIQ
                     PGLPVTITVDAFPNRPFEGTVLKIEPLAQVTQNVTQFPVLVNIGNPSHVLKPGMNTEV
                     EIHVGQRQNVLAVPNAALRTQGDVASAAQVLGLDPQAVQQQLAQAAAPAAAPRGDSGS
                     ASMGGGGTRGDPNPPQAGKPAQPPSTLTLPDGRQIPLPAGVTAEQVQAAMRKRFSGGE
                     LTPAEQAMLRQVFSQFRGGRGAGGPGGAMRSQTNEGGSRYIVFVLRNGAPAPVQIRTG
                     LTDLDYSEVTTGLTEKDTVLVLPSASLVNSQRQFQQRVQSMTGGGIPGMRQQTPQTQG
                     GGGTQRAPAPR"
     gene            complement(4898..5641)
                     /locus_tag="AC16JC_08670"
     CDS             complement(4898..5641)
                     /locus_tag="AC16JC_08670"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5164808.1"
                     /translation="MTAAAPMKFHTGETPHPDIVILTHRLTRNYDMGGEVVHALRGVD
                     IQIRRNEFVAVMGPSGSGKSTLMNLIGCLDTPTQGEYWLNGQKVSDLSDDELARIRNK
                     EIGFVFQTFNLLPRADSLHNVELPLIYAGVDAKTRRSAAAHALERVGLGDRMEHKPNE
                     LSGGQRQRVAIARALVNSPSIILADEPTGNLDSTTGEEIMRLFEELWSAGQTIVLVTH
                     EHDIAAHARRQIHLRDGLVERDVTMERTQ"
     gene            complement(5638..6909)
                     /locus_tag="AC16JC_08675"
     CDS             complement(5638..6909)
                     /locus_tag="AC16JC_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR5164809.1"
                     /translation="MVAGAGVAPLRAQQPDTAAPVTVQVSLEEAVRRALDVQPAMVQA
                     AGATRNAGAARRSAYGSFLPQVSVNGSASLNSSNRVDLNGGQIPPTWSYGSGLSASVD
                     LFTGLRRIWTLRSASAELSAADAGLITQRYQTILQTKQAFYNAIATEELVGVADAQVR
                     RAQLQLDVSVQKLHAGSATRSDSLRAAVDYGNARIALLQAQANLANAQANLGRQVGVD
                     QPVRALPDSVLPPPPDTTTLRSTLGQSAPQVAQADAQARAAQAGVWASRSQYIPSLRL
                     SYGLNRRDTLFSNVLIGNPTHNWQFALSWTLFNGFTREQNQVSSSVQRDNAAALAADT
                     RRMVNAQLTQQIAALETAYAQIEIAAVNVAAATEDLRVQQERYRVGAGTILDLLTSQA
                     ALTQAQTNLVQARFNYLIARAQLEALVGRSL"
     gene            complement(6930..7358)
                     /locus_tag="AC16JC_08680"
     CDS             complement(6930..7358)
                     /locus_tag="AC16JC_08680"
                     /inference="COORDINATES: protein motif:HMM:NF025173.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic heavy metal sensor"
                     /protein_id="HIR5164810.1"
                     /translation="MKPLDMLGRSRAWAVALLAAVFVAGGAAGWALGRHRPGGGGPSR
                     GGPDEIAAYLARRLDLTPVQQDSVRAVFTRHHAEMQTIWGAVRPRLDSLRAAVRTEVN
                     AQLTPDQQARYARLLADLEHQREGQRGRRDTTTSRQGQHE"
     gene            complement(7355..7702)
                     /locus_tag="AC16JC_08685"
     CDS             complement(7355..7702)
                     /locus_tag="AC16JC_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164811.1"
                     /translation="MSESLTPFNHRPDPVLGAALREALQPRDPAGGEAAFRARVLAAA
                     ERLPVPVVDVLARWARLGIAAAVAAALVTGMLVGRVGRQAESAVATTDPGAVVAGVQM
                     PDASLLLAGYSER"
     gene            complement(7699..8289)
                     /locus_tag="AC16JC_08690"
     CDS             complement(7699..8289)
                     /locus_tag="AC16JC_08690"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5164812.1"
                     /translation="MSTEPQRPDGAARTRRDADEAALAVRARSGDRAAFGELVERYAD
                     QARRVSRAVLQDADDADDAAQDAFLAALVKLHQYDAQRPFGPWLMRIVSNAATDRRRR
                     RTVRRVESLEEGLVAGGARPDAVAEREALRGRLRAALAQLPPRRSLAVTLFDVEGYSH
                     AEIAEMLGIPEGTVRSEVFHARRRLRTLLADWREAE"
     gene            complement(8279..9721)
                     /locus_tag="AC16JC_08695"
     CDS             complement(8279..9721)
                     /locus_tag="AC16JC_08695"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HIR5164813.1"
                     /translation="MSSQPSRATGREPERRDELALAIERAAHARPIEGNSVQHHPDSP
                     AALEAMLALIAGARRWVHFENYIIRNDRTGLRFADALIERARAGVHVCVLYDALGSFG
                     TSRAYWNALRRAGAEVRAFHPVSSPNLFDLFSRDHRKLAVVDGDRAMTGGLCIGDEWA
                     GNPARGRQPWRDTALMVCGPAAVALDAAFADAWEQAGPPLPVEDACGDPDICGDFTLQ
                     VVAGVPFEGRIYRATQLIAAAAQERLWITDAYLVPPAPLYAALSDAARHGVDVRLLVP
                     GASDLPLLRSFTRVGYRELLRAGVRVFEWRGAMMHAKTLVADRRWARVGSSNLNVSSL
                     FGNYELDLLADSQEAAEALAAQFRRDLLMSREVVLESRRRLPPRLVRAPASAEAGAHT
                     ELATTARHKRSRYELSAAAVVALRRVAGGLRRTLGGSAALFFLLLGVLLLLFPRVVGI
                     LIAAVMFWLSLGFAVYAFGKRRAREREDVD"
     gene            complement(9718..10308)
                     /locus_tag="AC16JC_08700"
     CDS             complement(9718..10308)
                     /locus_tag="AC16JC_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HIR5164814.1"
                     /translation="MNRTEALALMEQHTKNPALRQHMLAVEAVMRAYARKSGADEEAW
                     GAVGLLHDFDYEMYPNQEHSPTDGHPSWGVRLLRERGLSDEQCQAILGHAPYTGVPRA
                     SALARTLFAVDELCGFLVACVLVRPSRSFADLEVSSVKKKLKDKAFARGVSRDDVRVG
                     TEELGVPMDEHIGFSIEALRPVERTLGLGASVAPAG"
     gene            complement(10305..12128)
                     /gene="asnB"
                     /locus_tag="AC16JC_08705"
     CDS             complement(10305..12128)
                     /gene="asnB"
                     /locus_tag="AC16JC_08705"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR5164815.1"
                     /translation="MCGIFGVVSLDGAPIAHPDCLAAMAQSLAHRGPDGERIVGHDRA
                     RIGARRLAIMDLTTGDQPFQSPDGAVWMVCNGEIYNSPTLRREAEGWGYPFRSRGDIE
                     NIVPFYERLGPDAVARLDGMFGLAVWDDPRARLVLARDRAGEKPLFWTLADGELRFAS
                     EIQALLVFPDQPRRLDRAAAALYAALGYVPAPLTMLQGIRKLPPAHLLVAEGGNIAVR
                     PYWSPAIVASSPSRLGDAATLRAALLAAVERELMSDVPVGVFTSGGLDSSFLAGAASR
                     VMPGERIHTYAARFTARGYDESGFAADVTRDIRTAHHEVVADDAALHRAFEIITDRLA
                     EPVGDPAILPTYLLADAARHDVKVVLSGEGADELFGGYPTYLGHRLALAYGHLPRPLR
                     HAVRWTVERWPVSGGKVTFEFLLKQFVAGAEQPMVERHLTWFGALGPTPAMATELGRR
                     LEGFPPEPLSRMLWLDFLTYLPDNLLVKVDRGTMLASIEARAPYLDREVLELALPGAA
                     HLKVRRFTTKAILKEAAHGVVPERVIRRRKRGLSVPVARWLNAGLADVASRHLARPRL
                     FPGAPTAMLLAEHRAGTKNHARRLWPILMAELWAERWNVEP"
     gene            complement(12128..13849)
                     /locus_tag="AC16JC_08710"
     CDS             complement(12128..13849)
                     /locus_tag="AC16JC_08710"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5164816.1"
                     /translation="MRRGSISLDRASAPWLLLLAAAALALGWDLGGHRFLDPDEGRNA
                     EVAREMAETGDYVVPHLDGLPYLDKPVFYFAAAALGMEVLGPRETAARLPELLATLAT
                     IAVVVWFARRRWGSTAGWLAGVALATMPMVAAYARTTIFDSSLSFFTTSAIVCFAVER
                     PVLAWAAIGAGGITKGPVAILIPLATLIPWSLLTGTPVRRLFPWRGLLAFAVVALPWF
                     FAVTARIPEFPHYVFVRETFQRVTTRSFHRTAPLWYFLPILPLAAFPWIVPAVSRLSA
                     ARIRWGWLARKVNENARDPWLLAAWVLGPLVFFSLNQSKLPQYVLPLMPAFALAAART
                     LSREGAAVGWKAYVCAAAPLGLALATVTLWLPRSVPVTPAEQAAIPFTARVLAVALLV
                     SAGFVILAARRGSLPLASAGYALVTLALPIASGPLLRAVGDDRSAHSLAALIPDRASV
                     VAVAAYPPSLPFYLRRPILLATAHARELTSNYIADYADHYRTVPGSPLLPADAWRERL
                     TRCADPTVFVTRAGDRGARAALAQLPLLFADGHYAAYGPCTAPASPVDSAIHNPQSAM
                     RMGEGGS"
     gene            complement(13830..14258)
                     /locus_tag="AC16JC_08715"
     CDS             complement(13830..14258)
                     /locus_tag="AC16JC_08715"
                     /inference="COORDINATES: protein motif:HMM:NF046506.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6941 family protein"
                     /protein_id="HIR5164817.1"
                     /translation="MHLDFAVVADYALVDQTGKVSVLGIFQHIWVQQFPAMHPRLHLV
                     LRLKGKRTEIGEHRVQIRLADEAGAEILGGSGSITFAEPPAGVTEIEAPAILAFDVPF
                     AHPGMYRFEISIDGERAAEVPLSVSQMPPPGAVADAPGIH"
     gene            complement(14397..15593)
                     /locus_tag="AC16JC_08720"
     CDS             complement(14397..15593)
                     /locus_tag="AC16JC_08720"
                     /inference="COORDINATES: protein motif:HMM:NF022588.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HIR5164818.1"
                     /translation="MRIAVLPLAVLSLAAAPLAAQTERGAFVVTLGKDTIVIEQYTRT
                     RSTLEGDMLVRAAAVIQRHYTGSLKADGSIARFELTNRNLSNPQTPTVHFVVAFGDTT
                     VVDVTRDTTHNQIKVATPNGALPFLNFSYAMYEQYGRRAKATGKDTVATLPLGANSLT
                     MLVVHHPTRDSLNVAFAGDAPTLFRVDPSGLIQTVDGRFTTQKVLSTRLAALDFNALA
                     AAFATRPLGQLSPSDSVLATIGGAAITITYGRPSMRGRTVFGAESEMPRPIVPWNQVW
                     RTGANFATRFTSSTDLVVAGQTIPAGTYTLWTLPSPTGWKLIFNKQAKAPCATAAECA
                     DPKRANLWGTDYSADSDFVRVDMQTAALAAPLEQFVITLEPQGDGGVLAFAWEKTRAW
                     VNVAKK"
     gene            complement(15631..16080)
                     /locus_tag="AC16JC_08725"
     CDS             complement(15631..16080)
                     /locus_tag="AC16JC_08725"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012954953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5164819.1"
                     /translation="MPNKTAVMETGKGTIELELYADEAPNTVANFEKLANSGFYDGTK
                     FHRVIPNFMIQGGDPYSKTGKGPFGTGGPGYTIKCETDRNVHKHVVGTLSMAHAGKDT
                     GGSQFFICHSPQRHLDGVHTVFGQVTRGMDVVNAIKGNDALKSVRVK"
     gene            complement(16080..>16815)
                     /locus_tag="AC16JC_08730"
     CDS             complement(16080..>16815)
                     /locus_tag="AC16JC_08730"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5164820.1"
                     /translation="RVLIATKVGGNFYNRDVHPLLRDRIAQAAGVPYDRLPPDIPLPV
                     THDASFTADYIRFALGRSLVRLHTDYVDLLQLHNPPPQQIGAMETYAVLEDLKQEGKI
                     RAYGISVHSPEEGLAAIQSTMPDTVQLVYNLAQRQAESAFFPAARAANIGVIVREPLA
                     NGFLAGRYTPQSTWDNGDIRARMPQQHVARLAALGQRVRDLAAHTGSSPAQLALKFVL
                     DNAAVSTAIVGTKTVAQADENFTVET"
BASE COUNT         2464 a         5757 c         6080 g         2514 t
ORIGIN      
        1 gagcttcagg cgcgggatgt tggccgtgga gaaccggctg ccgtcgcggg cgcgcgtgat
       61 gcgctggtgc gtccacagcg cctcgatccg caccgactgc gtgggccgcc aggtgacggt
      121 cccgttcata tcgagctcac ggccttcggc ggcctcggcg aagatcggtg cggcgccgta
      181 gccgacgttc accgacagcg tgagggggcg gttgggcgtg ttcaggccga cgttcccgcc
      241 cagcaggtta tagaggccgt gcggcgtcac aaacggggtg cccgcgctgt cggtcgcgta
      301 cccgaggtag tccgcccggt cgaagcgctg ctggttgtcg ctgacgttga ccgacaggcc
      361 ccagccgccc cgcaggctgg cgttccagct gtcgctgaag ccgccttcga tcgtgctgcg
      421 caggtggagg aagtcgtcgt agtgccacag cgggtcgaac ccgacgaacg tggagacctg
      481 ctcgacgagc gcgcccggcc gcccgtacca gctgaagcgg ttgaagagcc ggatcgacac
      541 gtagtcggtc cgcgggacga acccggagcc cgccacgaag tccgggctga cgccctggat
      601 ctccccgtgg tttccatagg cgcgtccggt gcggtcgaaa gccgtcaggt cccacagggt
      661 tccggcccgg gcccctcccc cggccgggtc ccgcgtccac gacccgacca cctgggcctg
      721 cgtgaaccac agtttcttcc agaccacgcg cagatcggtg cccgcgaccc ggttgtaggc
      781 gctcccgtcc acgcggtcgg tatagacgag tccggcggtg gagctcgccc ccaggtcgcg
      841 ccgcagccgc aacaggttgt agacgggatt gctgcggccg aacgactgat cccggtcgtc
      901 caccgcggcg aggtaggcga cgttcgtgct cccgatcttc ccggtcagtt tcgctccccc
      961 cagcggctcc gccacccgtc gcgtgtagat cagccggttc ggcgagtcgt actgctccag
     1021 gccctcgagg aagaagggcc gtttctcggg gaagaacagc gggaagcgcg cgttcgccgt
     1081 cacctgtccg acgtcggcct cgacctgcga gaaatccgga ttggccgcgg ccgtcaggct
     1141 gaggttctcg gtgacgcccc agcgcagcgt gccgcccgca tcgggcgtcg tggtgtagcc
     1201 gtaggccgtg gcgccgggcg cgccgttgac cttggtcgtg acctccggcg tgatgtccat
     1261 gaccagtccg cgatgcagcc cggtgaggcc cgtgagccgt cccgactgga tgaggaagct
     1321 ggcgctggcg cgcacggccg gggtccaggt gtcctcgtag cccgagtgct ggatgacgcg
     1381 gtcgacctgg atgccccagg tctgcggatc ggcggattga tagtgcaggc tcttgaacgg
     1441 gatccgcact tccacctcgt agcccgtcgg ggtgacccgc ccgcgggatt cgaacacgaa
     1501 atcggggttc aggtcgatcg tggcgtcgaa ccgccctccc gcctgcgggc cgccggcgga
     1561 ggcctccacg ccgtcgctcc agaccccgtc ctcctgcacg ccgagcggat tgacggcgaa
     1621 cagcagggcg cggcggtggt cggcgtacgt gtccagcaac agctgcacgc ggtcgtccgc
     1681 gtcgatgttg tcgcggttgg cgagcgtcgc gcgcacgagg gcgccgccgt ggcgctcgaa
     1741 cgcccggatc ccgaaataga tcgcgtcggg cgcgtaccag accagcaccg cggtggaatc
     1801 ttccgccggc cggccgtccg agggccggta ctgcgagaac ccggtgagca cggccgcgcg
     1861 gctccagggt gcctcgtcga gcacgccgtc gatgcgcgcg gcggcttcga tgcggggcgg
     1921 taccacgtcc agctcgcggg cgctgccgcg atacacgggg cccgggtcgg acgatgtctg
     1981 cagcgcgagc aggagggcga gcatcgggga ataataaagc gtggggagtg gggagtggta
     2041 cgtactgggc tcggacggac aatgtcacct ctgacacgct ccggaccgtc actccccact
     2101 ccccactccc cgttgttctg ctcccgacta ttcgtagcgc agcgcgatga tcgggtcgag
     2161 cgaggccgcg cggcgagccg ggtagatccc gaagaacagc cccacgaggg cgctgaaggc
     2221 gaaggcgagg aagatcgaga agggcgagat cgccgtgttc cagtgcgcga ccttggagag
     2281 cacgaccgcg cccagtgagc cgaacagcac gccaacggtg cccccgacga ggcagagcac
     2341 gagcgcctcg accaggaact gcagcaggat gttccccttg gtggccccga gggccttgcg
     2401 cacgccgatt tcccgggtgc gctcggtgac cgacacgagc atgatgttca tgatcccgat
     2461 gccgcccacg agcagactga cggcggcgat ccccgcgagc aggaagccga aggtctcggt
     2521 cgtctgctgg aaggtcgaga gcacgtccgc ctggttgcgg atctggaagt cgttctcgcc
     2581 gccggcgcga atcttgtgtt ggcgccgcat gatgcgctcg acctcgatca tcgcgaggtt
     2641 catgttcttg acgctgtcca cctggaccgt gatcgagcgc agccggtcgg tgcccatgat
     2701 gcggtagcgc gccgtctgca gcgggatcag cacctgctcg tccggattct ggaacccctg
     2761 cgatcccttc tcgctcagga tgccgatgac ggtgaagggg atgccgcgga tctggatgtc
     2821 ccgtccgatc atcgcggccg cgttggcgtt gaacatgtcg gggacggccg aacccagcac
     2881 cgcgaaccgc cgccgcgcct cgtcctctcc cgtggtgaac atccgaccgg ccgtcagctc
     2941 gtacgcgtgc accttggcgt acgccgggat cgtgccgacc acgttgacgt tcacgttctg
     3001 gttgcccagc tgcacctgca tgttgctggc cagctcgggc accacggccg acacatagcg
     3061 ggcgttgtgc gccagggccg tgtcgtcgtc catggtgagg ctcacccggg tgttcatggc
     3121 gatgccgccc cggaagctct ggccggcgaa cagcgtcagc agcgtggggc ccaaggcctg
     3181 gatgcgggcc tcgatggctt tccgtgcgcc ggtgccgagc gcgatcatgg tgatcacggc
     3241 gccgacgccg atgatgatgc ccagcgccgt gagcagcgac cgcagcttgt tcgcgcggat
     3301 ggcctgcagc gcgacgcgga tcgtctcgtc gatgagcatc gctatctcgg cgcgggcgcg
     3361 cgctgagtgc cgccgccacc ctgggtctgc ggcgtctgct gccgcatgcc ggggatgccg
     3421 ccgcccgtca tcgactgcac gcgctgctgg aactgccgct gcgagttcac cagcgacgcc
     3481 gacgggagca ccagcacggt gtccttctcg gtcaggccgg tcgtgacctc cgagtagtcg
     3541 agatcggtga gcccggtccg gatctgcacc ggggccggtg ccccgttgcg cagcacgaag
     3601 acgatgtagc ggctgccgcc ttcgttggtc tggctccgca tcgcgccgcc gggcccgccc
     3661 gctccccggc caccacggaa ctgcgagaac acctggcgca gcatggcctg ctcggccggg
     3721 gtcagctcac cgcccgagaa gcgcttgcgc atcgccgcct gcacctgttc ggcggtcact
     3781 ccggcgggca gcggaatctg gcggccgtcg gggagcgtga gggtcgacgg cggctgcgcg
     3841 ggcttgcccg cctgcggcgg gttcgggtcg ccgcgcgtgc cgccgccgcc catcgaggcg
     3901 ctgccgctgt cgccgcgggg cgccgcagcc ggcgccgcgg cctgggcgag ctgttgctgc
     3961 accgcctggg ggtcgagccc cagcacctgg gcggcggagg cgacgtcgcc ctgcgtccgc
     4021 aacgccgcgt tggggacggc cagcacgttc tggcgctgcc ccacatggat ctccacttcc
     4081 gtgttcatcc ccggcttcag cacgtggctc gggttgccga tgttcacgag cacggggaac
     4141 tgcgtcacgt tctgcgtgac ctgcgccagc ggctcgatct tgagcaccgt gccctcgaag
     4201 ggacggttgg ggaacgcgtc gaccgtgatc gtgaccggaa gtcccggctg gatcttgccg
     4261 atgtcggtct cgtccaccag ggcctgcacc tgcacggtgt cgaggttcgc catgcggaac
     4321 aggaccgtgc cgccgcccac gtcgcgggtg gggctcgaga tcacggtgcc gagctccacg
     4381 ttcttctgga tgaccgtccc gttgaggggg gcggtcacgc gtgtctgatc ccaggcgatc
     4441 tgcgcgtccg ccagattgga ctgggcgttg acgagccccg cctgcgcctg ggcgtaggcg
     4501 accttggccg tttcgtactc ggcctgggtg atggcctggg tcttgagcag cgtgtcctcg
     4561 cgggccagct gggtcgaggc gttctcgaac tgcgcccggg cgacggcgag attcgcccgt
     4621 gcctggtcga gtgcgctctt ggggagtcgg ggatccacca gggcgagcag ctgccccggg
     4681 cggacatcgt cgccggtctg tgcccgcatc tcggtgatgg cgccggacgc ctgcgatttc
     4741 acgtccaccg tcaggagcgg ttggatcgcg ccggcggccg aggcggtcac gacgatgtcg
     4801 cggcgctcca cggcgacctt ctcgtacacg ggcgtgggct cgggcttttt gcaggcggtc
     4861 caaccggccg ccgcgatcgc cgtcagcaga agtcgcttca ctgggttcgc tccatcgtca
     4921 cgtcccgctc gacgagaccg tcgcgcaggt ggatctggcg gcgcgcgtgc gccgcaatgt
     4981 cgtgctcgtg ggtgaccagg acgatggtct gtccggccga ccagagctcc tcgaagagtc
     5041 gcatgatctc ctcgccggtg gtcgaatcga ggttgccggt cggctcgtcg gcgaggatga
     5101 tgctggggct gttcacgagc gcccgggcga tggccacgcg ctggcgctgg ccgccggaca
     5161 gctcgttggg cttgtgctcc atgcggtcgc ccaggccgac gcgctccagg gcgtgggccg
     5221 cggcgctgcg gcgcgtcttg gcatccacgc cagcgtagat cagcggcagc tccacgttgt
     5281 ggagcgagtc cgcgcggggc agcaggttga acgtctggaa cacgaagccg atttccttgt
     5341 tgcggatccg cgccagctcg tcgtcggaca aatcgctcac cttctggccg ttgagccagt
     5401 attccccctg ggtcggtgtg tcgaggcagc cgatgaggtt catcagggtc gacttgcccg
     5461 acccggaggg gcccatcacc gccacgaact cgttgcggcg gatctggatg tccacgccgc
     5521 gcagcgcatg caccacctcg ccccccatgt cgtagttcct ggtgaggcgg tgggtgagga
     5581 tgacgatgtc cgggtgcggc gtttcgccgg tgtggaattt catcggagcg gcggcggtca
     5641 tagggagcgt cccacgagcg cttcgagctg cgcccgcgcg atgaggtagt tgaagcgcgc
     5701 ctggaccaga ttggtctgcg cctgcgtgag ggcggcttgc gacgtcaaca ggtcgaggat
     5761 cgtgccggcg ccgacccggt agcgctcctg ctggacgcgc agatcctcgg tggccgccgc
     5821 gacgtttacc gcggcgatct cgatctgggc gtatgcggtc tccagcgcgg cgatctgctg
     5881 ggtcagctgg gcgttcacca tgcggcgcgt gtcggccgcc agcgctgcgg cattgtcgcg
     5941 ctggacgctg gaagagacct gattctgctc gcgtgtgaat ccgttgaaca gcgtccagga
     6001 cagcgcgaac tgccagttat gcgtgggatt cccgatcagc acgttcgaga agagcgtgtc
     6061 ccgtcgattc agaccgtagg acaatcggag ggacggaatg tattgcgagc ggctggccca
     6121 gacgccggcc tgcgcggccc tggcctgggc gtccgcctgg gcgacctgcg gcgcggattg
     6181 ccccagcgtg ctgcgcagcg tcgtcgtgtc cggcggcggc gggagcacgc tgtcggggag
     6241 cgcgcgcacc ggttggtcta cgccgacctg tcgacccaga ttcgcctggg cgttcgcgag
     6301 gttcgcctgc gcctgcagca gggcgatgcg tgcgttgccg tagtcgacgg ccgctcgtaa
     6361 cgagtccgaa cgggtcgccg atcccgcgtg cagcttctgg accgacacgt cgagctgcaa
     6421 ctgggcgcgg cgcacctgtg cgtcggccac acccacgagc tcctccgtgg cgatcgcgtt
     6481 gtagaaggcc tgcttcgtct gcaggatcgt ctgatagcgc tgggtgatca gtcccgcatc
     6541 ggcggcgctc agctctgccg acgccgagcg cagggtccag atccggcgga gaccggtgaa
     6601 caggtctacc gaggccgaca ggcccgagcc gtagctccag gtcggaggaa tctgcccgcc
     6661 gttcaggtcg acgcggttcg agctgttgag agaggccgac ccgttcacgg agacctgcgg
     6721 caggaatgac ccgtaggccg accggcgtgc ggcccccgca ttgcgggtgg cgcccgccgc
     6781 ctgcaccatg gccggctgga cgtcgagcgc ccggcgcacg gcctcctcca ggctcacctg
     6841 cactgtcacc ggcgccgccg tatcgggctg ctgcgcccgc agcggcgcga cgccggcgcc
     6901 cgccaccatc agtactaggg aaagtcccct cattcgtgct gcccttgtct cgaagtagta
     6961 gtgtcccggc gcccgcgctg cccctcacgc tgatgctcga ggtcggccaa gagtcgcgca
     7021 tagcgggctt gctgatcggg tgtgagctgc gcgttgacct ccgtgcgcac ggccgcccgc
     7081 agggaatcga gccggggccg gaccgcgccc cagatggtct gcatctccgc gtgatggcgc
     7141 gtgaacaccg cgcgcaccga atcctgctgc accggcgtga ggtcgagacg gcgcgcgagg
     7201 tacgccgcga tctcgtcggg tcccccgcgc gacggtcccc cgccgcccgg acggtgccga
     7261 ccgagtgccc agccggccgc gccgccggcc acgaacacgg ccgcgagcag cgccaccgcc
     7321 caggcgcggg agcgccccag catgtccagg ggcttcaccg ctcggaatac cccgccagca
     7381 gcaggctcgc gtccggcatc tgcaccccgg cgaccaccgc gccgggatcg gtcgtggcca
     7441 cggccgactc ggcctggcgg ccgacccgtc cgacgagcat cccggtcacc aacgccgccg
     7501 cgaccgccgc cgcgataccc agacgtgccc aacgggccag cacgtccacg accggaacgg
     7561 gcaaccgctc ggcggccgcc agcacgcgcg cacggaacgc cgcttccccc ccggcggggt
     7621 cgcgcggctg caacgcctcc cgcagcgcgg cccccagcac cggatcgggc cggtggttga
     7681 acggcgtcag tgattcgctc attccgcttc cctccaatcg gcgagcagcg tccgcaaacg
     7741 ccgccgcgcg tgaaacacct cggagcgcac ggtcccctcg gggatcccca gcatctcggc
     7801 gatctcggcg tgggagtatc cctccacgtc gaacagcgtg accgccagac tgcgccgcgg
     7861 cggcagctgt gcgagcgccg cgcgcaaccg gccgcgcaac gcctcccgct ccgccacggc
     7921 atcgggtcgc gcgccgcctg cgaccagacc ttcctccagc gactcgacgc gccgcacggt
     7981 ccgccggcgc cgccggtccg tggccgcatt cgacacgatg cgcatcagcc acggcccgaa
     8041 cggccgctgc gcgtcgtact ggtgcagctt cacgagcgcg gcgagaaacg cgtcctgcgc
     8101 ggcgtcgtcg gcgtcgtcgg catcctgcag caccgcccgc gacacgcggc gggcctgatc
     8161 ggcgtagcgc tcgaccagct ccccgaaggc cgcccggtcg cccgagcggg cgcggacggc
     8221 cagtgccgct tcgtccgcgt ccctccgtgt acgcgccgcc ccgtccgggc gttgaggctc
     8281 agtcgacatc ttcacgttct cttgcgcggc gcttcccgaa ggcatacacc gcgaacccca
     8341 gcgacagcca gaacatcacg gcggcgatca ggatccccac gacacgggga aacaggagga
     8401 gcaggacccc gagcagcaga aagaagagcg cggcgcttcc ccccagcgtg cggcgcaggc
     8461 cgccggctac ccggcgcagg gcgaccaccg ccgccgcgct gagctcgtag cgggagcgtt
     8521 tgtgccgcgc cgtcgtcgcc agctcggtgt gcgccccggc ctccgccgat gccggcgcgc
     8581 gcacgaggcg cggaggcaga cggcggcggg attccagcac cacctcccgg ctcatgagca
     8641 ggtcgcgccg gaattgcgcg gcgagcgctt cggccgcctc ctggctgtcg gcgagcaggt
     8701 cgagctcgta attgccgaac aggctcgaga cgttcaggtt gctcgagccg acgcgcgccc
     8761 agcggcggtc ggccacgagc gtcttggcgt gcatcatggc cccgcgccac tcgaacacgc
     8821 gaacgccggc ccgcaacagc tcgcggtacc ccacgcgcgt gaaactgcgc agcagcggga
     8881 gatcgctcgc gccggggacg agcagccgca cgtccacgcc gtggcgcgcg gcgtcgctca
     8941 acgcggcgta gaggggcgcg ggcgggacca ggtaggcgtc ggtgatccac agccgttcct
     9001 gcgccgcggc ggcgatcagc tgggtggccc gatagatccg tccttcgaac ggcacccccg
     9061 cgacgacctg caacgtgaag tcgccgcaga tgtcggggtc gccacaggcg tcctcgactg
     9121 ggagcggcgg gcccgcctgc tcccacgcat cggcgaacgc tgcgtcgagc gccaccgcgg
     9181 ccggaccgca gaccatgagc gccgtgtcgc gccacggctg ccgtccgcgc gcggggtttc
     9241 ccgcccactc gtccccgatg cacaggccgc ccgtcatggc gcgatcgccg tccaccaccg
     9301 cgagcttgcg gtgatcgcga ctgaacaaat cgaacaggtt cggggaggag acgggatgaa
     9361 acgcgcgcac ctcggcgccg gcgcggcgca gcgcgttcca gtacgcgcgc gacgtcccga
     9421 acgatccgag cgcgtcgtag aggacgcaca cgtgaacgcc ggcgcgcgcg cgctcgatga
     9481 gcgcatcggc gaaccggagg ccggtccggt cgttccggat gatgtagttc tcgaagtgga
     9541 cccagcggcg ggcgcccgcg atgagggcga gcatcgcctc gagcgccgcc gggctgtcgg
     9601 ggtggtgctg gaccgagttc ccctcgatgg gccgggcgtg cgccgcgcgc tcgatggcca
     9661 gtgcgagctc gtcccggcgc tcgggctccc ggccggtggc gcgcgatggt tgcgagctca
     9721 ccccgcgggc gccacgctcg cccccaggcc gagcgtccgc tcgaccggtc gcagggcctc
     9781 gatgctgaag ccgatgtgct cgtccatggg cacgccgagc tcctcggtcc ccacccgcac
     9841 gtcgtcgcgg ctgacgccgc gggcgaacgc cttgtccttg agcttcttct tgaccgacga
     9901 cacctcgagg tcggcgaagc tgcgcgacgg ccgcaccagg acgcaggcca ccaggaagcc
     9961 gcacagttcg tccaccgcga acagcgtccg cgccaaggcc gaggcgcgcg gcacgccggt
    10021 gtatggagcg tgccccagga tcgcctggca ttgctcgtcg gagagtccgc gctcgcgcag
    10081 cagccgcacg ccccagctgg ggtgcccgtc ggtgggggaa tgctcctggt tcgggtacat
    10141 ctcgtagtcg aagtcgtgca gcagcccgac ggcgccccac gcctcctcgt ccgcgccgga
    10201 tttccgggcg taggcccgca tgacggcttc caccgcgagc atgtgctggc gcaacgcggg
    10261 gttcttggtg tgctgctcca tcagcgccag ggcttcggtt cgattcatgg ctccacgttc
    10321 cagcgttccg cccacagctc ggccatcagg atgggccaca gccgccgggc gtgattcttg
    10381 gtacccgcgc gatgctcggc cagcagcatc gccgtcggcg ccccgggaaa caggcgcggg
    10441 cgcgccagat gccggctggc cacgtcggcg agaccggcgt tcagccaccg cgccaccggt
    10501 accgacagcc cgcgcttgcg ccgccggatc acccgctccg gcacgacgcc gtgcgccgcc
    10561 tccttcaata tcgccttggt ggtgaagcgg cgcaccttga gatgggcggc tcccggcagc
    10621 gcgagctcca ggacctcccg gtccaggtac ggcgcccggg cctcgatgga ggcgagcatg
    10681 gtgccccggt ccactttgac gaggagattg tccgggagat acgtcaggaa atcgagccag
    10741 agcatccggc tcagcggctc cggggggaag ccctccaggc gacgccccag ctccgtcgcc
    10801 atcgcgggcg tgggtcccag cgctccgaac cacgtcaggt ggcgctccac catgggttgc
    10861 tccgcaccgg ccacgaattg cttgagcaga aactcgaacg tgaccttgcc gccggacacg
    10921 ggccaccgct cgaccgtcca gcggacggcg tggcgcagcg gacgcggcag atgcccgtag
    10981 gcgagcgcga gccggtggcc gaggtaggtg gggtacccgc cgaacagctc gtccgctccc
    11041 tcgcccgaga gcaccacctt cacgtcgtgc cgcgccgcgt ccgccagcag atacgtcggc
    11101 aggatcgcgg ggtcgcccac cggttcggcg agacggtccg tgatgatctc gaacgcgcgg
    11161 tgcagcgcgg cgtcgtcggc cacgacctcg tggtgggccg tacgaatgtc gcgcgtaacg
    11221 tccgcggcga agccgctctc gtcgtagccg cgggcggtga accgcgccgc ataggtgtga
    11281 atgcgttctc ccggcatcac gcgcgacgcc gccccggcga ggaaggacga atccagaccg
    11341 ccgctcgtga agacgccgac cggcacgtcg gacatcaact cgcgctccac cgcggcgagc
    11401 agcgccgcgc gcaacgtcgc ggcgtcgccc aggcgtgagg gcgacgacgc cacgatggcg
    11461 ggagaccagt aggggcgcac ggcgatgttc ccgccctccg ccacgagcag gtgggccggg
    11521 ggaagcttgc gaatgccctg cagcatcgtg agcggcgccg ggacgtagcc cagcgcggcg
    11581 tacaacgccg cggccgcacg gtccaggcgg cgcggttgat cggggaagac gagcagcgcc
    11641 tgtatctccg aggcgaagcg cagttcgccg tcggccagcg tccagaacag cggcttctcg
    11701 ccggcacggt cccgcgccag caccagccgc gcccgggggt cgtcccacac ggcgaggccg
    11761 aacatcccgt cgaggcgggc cacggcgtcg gggccgagcc gctcgtagaa cggcacgatg
    11821 ttctcgatgt cgccgcgtga tcggaacggg tacccccagc cttccgcctc gcgccgcagc
    11881 gtcggggagt tgtagatctc gccgttgcac accatccaga cggcaccgtc gggactctgg
    11941 aagggctgat cgcccgtggt caggtccatg atggcgagcc gccgcgcccc gatgcgggcg
    12001 cgatcgtgcc cgacgatgcg ctcgccatcg ggcccgcggt gggccagcga ctgggccatt
    12061 gccgcgagac agtcgggatg cgcgatcggg gcgccgtcga gggaaacgac gccgaagatg
    12121 ccgcacacta gctccccccc tcccccatcc tcattgcgga ttgcggattg tggattgcgg
    12181 aatcgaccgg ggaagccggt gcggtgcagg gaccataggc agcgtaatgg ccgtcggcga
    12241 agaggagagg cagctgcgcc agcgcggccc gggcgcctcg atcgccggcg cgcgtcacga
    12301 acaccgtagg atcggcgcag cgggtgagac gctcgcgcca ggcatcggcc gggagcaggg
    12361 gcgagccggg gaccgtgcga tagtgatcgg cgtagtccgc gatgtagttg ctcgtgagct
    12421 cacgggcgtg cgccgtcgcg aggaggatcg ggcggcgcag gtagaacggc agcgacggcg
    12481 gatatgcggc gacggcgacg acggacgccc ggtccgggat cagcgcggcc aggctgtggg
    12541 ctgaacggtc gtcgcccacc gcccgcaaca gggggcccga ggcgatgggg agcgcgagcg
    12601 tcacgagggc gtagccggcg gaggcgagcg gcaggctacc acgccgggcc gcgaggatca
    12661 cgaacccggc cgagacgagc agcgccacgg cgagcacccg ggcggtgaag gggatcgccg
    12721 cctgctcggc cggcgtgacg gggaccgagc ggggcagcca cagcgtgacg gtcgccagcg
    12781 ccaggccgag cggcgcggcg gcgcagacgt aggccttcca gccgacggcg gctccctcgc
    12841 gggagagtgt gcgcgccgcc gccagggcga atgccggcat gaggggcagc acgtactgcg
    12901 gcagtttcga ctggttcagc gagaagaaca ccaggggccc cagcacccag gcggcgagca
    12961 gccacgggtc gcgcgcgttc tcgttcactt tgcgcgcgag ccagccccag cggatccgtg
    13021 cggcgctgag tcgtgacacc gccggcacga tccaggggaa cgccgcgagc ggcaggatcg
    13081 ggaggaaata ccacaacggc gcggtccggt ggaagctccg cgtggtcacc cgctggaacg
    13141 tctcgcgcac gaagacgtag tgggggaact ccggaatgcg cgcggtcacg gcgaagaacc
    13201 acggcagggc gaccacggcg aacgcgagca gcccgcgcca cgggaacagg cgccgcaccg
    13261 gcgtgccggt gagcagggac caggggatca gggtggcgag agggatgagg atcgcgacgg
    13321 gtcccttggt gatcccgcct gcgccgatcg ccgcccaggc gaggaccggg cgctccacgg
    13381 cgaaacagac gatggccgat gtggtgaaga aggaaaggga gctgtcgaag atcgtggtgc
    13441 gggcgtaggc cgccaccatc ggcatggtgg ccagggccac tcccgcgagc caccccgccg
    13501 tgctgcccca gcgccggcgg gcgaaccaca cgacgacggc gatggtcgcg agggtggcga
    13561 gcagttcggg gagacgcgcc gccgtctccc ggggaccgag tacctccatg ccgagggcgg
    13621 cggccgcgaa gtagaagacc ggcttgtcga ggtaggggag cccgtcgagg tgggggacga
    13681 cgtagtcgcc ggtctccgcc atctcgcggg cgacctcggc gttgcggcct tcatcgggat
    13741 cgaggaagcg gtggccgccc aggtcccagc cgagcgccag cgcggccgcg gcgaggagca
    13801 gcagccaggg ggcggaggcg cgatccaggc taatggatcc ccggcgcatc ggccaccgcg
    13861 ccgggcggcg gcatctggct gacgctgagc ggaacctccg cggcgcgctc gccgtcgatg
    13921 ctgatctcga aacggtacat cccggggtgt gcgaagggca cgtcgaaggc gagaatcgcc
    13981 ggggcttcga tttcggtcac gccggccggg ggctcggcga aggtgatgct gccgctgccg
    14041 ccgaggatct ccgcgcccgc ttcgtcggcg aggcggatct gcacccggtg ctcgccgatc
    14101 tcggtgcgtt tccccttcag gcgcagcacg aggtgcaggc gcgggtgcat cgcggggaac
    14161 tgctgcaccc agatgtgctg aaagatgccc aggaccgaaa ccttcccggt ctgatcgacg
    14221 agggcgtaat ccgcgacgac ggcgaaatcc aggtgcatgg aggtgcgtgg tccgtggtgt
    14281 gtggtactcg acggtccggg gccgtcaacc ggttacgctt gccgccgtcg gagccgcggc
    14341 caagattgcc accggataat accacgcatc acgcaccacg cactacgcgg ccacactcac
    14401 ttctttgcga cgttcaccca cgcgcgcgtc ttctcccagg cgaaggcgag cacgcccccg
    14461 tcgccctgcg gctcgagggt gatgacgaat tgctcgagcg gcgccgccag cgcggcggtc
    14521 tgcatgtcga cccggacgaa gtcggaatcg gcgctgtagt cggtgcccca gaggttggcc
    14581 cgcttggggt cggcgcactc ggccgcggtg gcgcagggtg ctttggcctg cttgttgaag
    14641 atcagcttcc agccggtggg tgagggcagg gtccagagcg tataggtccc ggcgggaatc
    14701 gtctggccgg ccacgacgag gtcggtggag ctggtgaatc gcgtggcgaa gttcgcgccc
    14761 gtgcgccaca cctggttcca cgggacgatg gggcgcggca tctcgctctc ggcgccgaac
    14821 accgtgcggc cgcgcatcga gggacgtccg taggtgatgg tgatcgcggc cccgccgatg
    14881 gtggcgagca ccgaatccga cggcgaaagc tgcccgagcg gccgggtcgc gaaggcggcg
    14941 gcgagagcgt tgaaatcgag cgcggcgagc cgggtggaga ggaccttctg ggtcgtgaac
    15001 cggccgtcta ccgtctggat cagcccgctg gggtcgaccc ggaacagcgt gggcgcgtcg
    15061 cccgcgaacg cgacgttgag cgagtcgcgg gtggggtggt gcaccaccag catcgtcaga
    15121 gaattcgcgc cgaggggcag cgtggccacg gtgtccttgc ccgtcgcctt ggcacgccgg
    15181 ccgtactgct cgtacatggc gtaggaaaag ttgaggaacg gcagggcgcc gttcggcgtc
    15241 gcgaccttga tctggttgtg cgtcgtgtcg cgggtcacgt ccacgaccgt cgtgtcgccg
    15301 aacgcgacga cgaagtgcac ggtcggggtc tgcgggttgc tgaggttgcg attcgtgagt
    15361 tcgaagcgcg ctatcgagcc gtcggctttc agcgagccgg tgtagtgccg ctggatcacg
    15421 gccgcggcgc gcaccagcat gtcgccctcg agcgtcgagc gcgtgcgggt gtactgctcg
    15481 atcacgatgg tgtccttgcc gagcgtgacg acgaacgcac cgcgctcggt ctgcgccgcg
    15541 agcggcgcgg cggcgaggga caggacggcg agcggcagga ctgcgatacg catgacggtt
    15601 ctccctgtgg gggtcgaggg cggccgggcg ctacttcacc cgcaccgact tcaatgcatc
    15661 gttccccttg atcgcgttga caacgtccat tcccctggtg acctgtccga aaacggtgtg
    15721 gacgccgtcc agatgccgtt gcggcgaatg gcagatgaag aactggctgc ccccggtgtc
    15781 cttgcctgcg tgggccatcg agagggtgcc gacgacgtgc ttgtggacgt tgcggtccgt
    15841 ctcgcacttg atcgtgtagc cgggcccgcc cgtgccgaag ggccccttgc cggtcttcga
    15901 ataggggtcg ccgccctgga tcatgaagtt ggggatgacg cgatggaatt tggtgccgtc
    15961 gtagaacccg gaattggcga gcttctcgaa gttggcgacg gtgttggggg cctcgtcggc
    16021 gtacagctcg agctcgatcg tccccttgcc ggtctccatc acggcggtct tgttgggcat
    16081 caggtctcca cggtgaagtt ttcgtcggcc tgcgcgacgg tcttggtccc cacgatcgcc
    16141 gtcgagaccg cagcattgtc gagcacgaat ttcagcgcca gctgggcggg gctggacccg
    16201 gtatgcgcgg ccaggtcgcg aacgcgctgg cccagcgccg cgagccgggc cacgtgctgc
    16261 tgcggcatgc gcgcccggat gtcaccgttg tcccaggtac tctggggagt gtagcggccg
    16321 gccagaaagc cgttggccag gggctcgcgc acgatcaccc cgatgttcgc ggcccgcgcg
    16381 gcggggaaga acgcggactc cgcctgccgt tgcgcgagat tgtacaccag ctgcaccgtg
    16441 tcgggcatgg tggactggat ggcggcgagc ccctcctcgg gtgagtgcac cgagatgccg
    16501 taggcccgga tcttcccttc ctgcttgagg tcctccagca cggcgtaggt ctccatcgcg
    16561 ccgatctgct gtggcggcgg attgtgcagc tgcagcagat cgacgtagtc cgtgtgcagc
    16621 cgcacgagcg agcggccgag ggcgaaccgg atgtagtccg ccgtgaagct ggcgtcgtgg
    16681 gtgaccggca gcgggatgtc gggcggcagg cggtcgtacg ggacccccgc ggcctgggcg
    16741 atccggtcgc gcagcagggg atggacgtcg cggttgtaga agttgccgcc gaccttggtg
    16801 gcgatgagca cccgg
//
LOCUS       DBNGZW010000372         4027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_579669, whole genome shotgun sequence.
ACCESSION   DBNGZW010000372 DBNGZW010000000
VERSION     DBNGZW010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_579669"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..1413)
                     /locus_tag="AC16JC_08735"
     CDS             complement(115..1413)
                     /locus_tag="AC16JC_08735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5164821.1"
                     /translation="MSTSACLRTLCILLVAGRAVHAQTPVDSGLAAYIATIRAIDGHA
                     HPMRPVTPGAPADTEFDALPLDGIPPFPAPWRLTLEAPVWSRAARTLYGSDGPEARAR
                     TVRERGEAFPTWALDRAGIDVMFANRVAMGPGLGPPRFRWVPFDDALLFPLDTRLEAA
                     RTPDTRSLYPREAALLRRDLRDLGLDALPATLDAYLAAVVLPTLRRQRERGAVAIKFE
                     AAYLRPLDFDDPDSTAARAVYARYVGGGTPAHAEYKTLADYLFRMIAREAGRQGLAVH
                     LHVLETFGGFYGARGASAGLLEPVFNDSTLRGTNFVIIHGGWPETGETEAMLGKPNVY
                     ADISMMDLILEPAELAEVLRHWLARWPDKVLFGTDAFDGGPAQPWEEGAIVASMTARR
                     ALGMALTAMVRDGDVDAARARALARMVLRDNAAALYHLGP"
     gene            1780..3900
                     /locus_tag="AC16JC_08740"
     CDS             1780..3900
                     /locus_tag="AC16JC_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164822.1"
                     /translation="MDQLLIHPQGALLCVSPSEPDTLVDSAAVGSARWRRDTISIRNC
                     GGGELRWTASVTEGNPWLAILPDSGVAGSAPQVVFDPASLVDGVYRGMIEVVSTTGSG
                     VAEIPVGFFVHPCRVTPITIDDSAAATLSAEDCGAPHRTGSFARVYGFPGTANDLVSI
                     EVSAGFNAWVALDTALHAGSPTLAEAGDCLRVAGDPCLYYLRLPRNQTYFIEMTSAAA
                     ADSGAYRLRLVHPRLPDGPNGLNQLLSDSVTSVAAGATVNATGILLRAVVSDPDLGDS
                     LQLEGEVRPTGIAFTGPNVPDGPVAGNGQTTFLSVGGLIDGTTYHWRVRARDNTGRSG
                     PWVAFGGNPDFVVNVLHPPNAPTTLGQATAGGAVLLTGATTDTNMVRLGAVVSDPDAG
                     DQLRLEVEVRPVGVPFTAPTNASAPAVDGGALQVLVGPLPGATSYHWRARAVDQTGDT
                     SSWVAYGGNAESAADFTIAAQQHAAYPPTSLAQLRSSDGSPIPVGGAVASTSIVLAAV
                     VSDLDPTRTLQLEVEVQPVGQGFLGRPTSSSAFVASGTVASVSVGSLTASTGYHWQAR
                     ARDDGADTSAWVSFPEGPGNAETDPDFVVQTPPVRLVFTVQPSNAVVGTAIIPAIEVT
                     ALDANGQVVTAYSDSVAMTIATNPSGGRLSGTTTVVAVAGVARFADLKINKAGTGYTL
                     QAATRSPALSVISVPFDITPKTKP"
BASE COUNT          621 a         1384 c         1440 g          582 t
ORIGIN      
        1 agaggacctc aggcgatgcc cagggtcctc cgaatcggtc actggtcttc agccgacggc
       61 accatgccgc agctctacgc tcggcaagaa gtatccccac aacgcgcgca aaagtcaagg
      121 ccccagatga tacagcgcag cggcgttgtc gcgcagcacc atgcgcgcca gagcgcgggc
      181 gcgcgccgcg tcgacgtccc cgtcgcgcac catggcggtg agcgccatcc cgagcgcacg
      241 ccgcgcggtc atagacgcca cgatcgcgcc ctcctcccac ggctgggccg ggccgccgtc
      301 gaacgcatcg gtgccgaaca ggactttgtc gggccagcgc gccagccagt gccgcagcac
      361 ctccgccagc tccgccggct cgaggatcag gtccatcatc gagatgtcgg cgtacacgtt
      421 cggcttcccg agcatcgcct cggtctcgcc ggtctcgggc cagccgccgt ggatgatcac
      481 gaagttcgtg ccccgcagcg tcgagtcgtt gaacacgggc tccagcaatc cggcgctcgc
      541 gccgcgtgcg ccgtagaatc caccgaacgt ctcgagcacg tgcaggtgga cggcgaggcc
      601 ctgccgtccc gcctcgcgcg cgatcatccg gaagaggtag tcggcaagcg tcttgtactc
      661 ggcatgagcg ggcgtgccgc cgccgacata gcgcgcatag acggcgcgcg ccgcggtcga
      721 gtcgggatcg tcgaaatcca gcggccgcag atacgccgcc tcgaacttga tcgccaccgc
      781 tccgcgttcc cgctggcgtc gcagcgtcgg gagcaccacg gccgccaggt aggcgtcgag
      841 ggtggccggg agcgcgtcga gtcccaggtc ccgcaggtcg cgacggagca gggccgcctc
      901 gcgcggatac agcgaccgcg tatcgggcgt gcgtgccgcc tcgagccggg tgtcgagcgg
      961 gaagaggagc gcgtcgtcga acggcaccca gcggaagcgc ggaggcccga gtcccggtcc
     1021 catggctacg cggttggcga acatcacgtc aatgcccgcg cggtcgagcg cccaggtggg
     1081 aaaggcctcg ccgcgctcgc gcaccgtccg cgcgcgcgcc tcggggccgt ccgatccgta
     1141 gagcgtgcgc gccgcccggg accacaccgg ggcttcgagc gtcaggcgcc agggcgcggg
     1201 gaacggcggg atgccgtcga gcggcagcgc gtcgaattcc gtgtcggccg gggcgccggg
     1261 agtcacgggt cgcatggggt gggcatggcc gtcgatggcg cgaatcgtcg cgatgtaggc
     1321 cgccaggccg gaatccaccg gcgtctgggc gtgcaccgcc cggccggcga cgagcaggat
     1381 gcacagggtg cggaggcagg ccgaggtgct catgcgtacg gcagtgacga tacggcggcg
     1441 ggacgcgcgg ggaaggcctc cccgcccgtc gcgccgtcga cgcccagtgg ccccgttcta
     1501 agagtaaatc agacaggagg ttatcgacgc ctcacgggtc attcaaccgc gtcgaaggcc
     1561 tcgatcacga cggtacgggc tgtgctccaa aagtggccaa gtgcaaggtc gcgcagtctt
     1621 tagagactca gatccaatta agcaggaatt cagatactca ctagagattg ggatccggat
     1681 atcaagcgaa tgggtgcagc cgtccgattg ccagtctcgt ggcgtccgtg gtgcggacgc
     1741 ctcgctttga tcgcggcgct cacgccgacg gcctgccgag tggaccaact cctgatccat
     1801 ccccagggcg cgctgctctg cgtctctcct tccgagccgg acacgctggt cgactcggcc
     1861 gccgtcggca gtgcccggtg gcgcagggac accatcagca tccgaaactg cggtgggggc
     1921 gagctccgct ggacggcgtc ggtcacggag gggaatccgt ggctggccat tctgccggat
     1981 tccggcgtcg ccgggagcgc gccgcaggtg gtattcgatc ccgcatcgct ggtcgacggg
     2041 gtgtaccgtg ggatgatcga ggtcgtgtcg acgaccggga gcggcgtcgc ggagatcccg
     2101 gtggggttct tcgtccatcc gtgtcgcgtc acgccgatca cgatcgatga ttccgccgcc
     2161 gccacgctgt ccgcggagga ctgcggcgcg ccgcaccgaa cgggcagctt cgcgcgggtc
     2221 tatggattcc ccggcaccgc caacgacttg gtctcgatcg aggtctcggc cggattcaac
     2281 gcctgggtgg cgctcgacac tgcgttgcac gccgggagcc cgacgctcgc cgaagccggg
     2341 gactgcctgc gcgtcgcggg cgacccatgt ctctattatc tgcgcctgcc gcgaaaccag
     2401 acgtacttca tcgagatgac gagcgctgca gccgccgact ccggggccta ccggctgcgc
     2461 ctggtgcatc cccggttacc tgacggcccc aatgggctca atcagttgct gagcgactcg
     2521 gtgaccagtg tggccgccgg tgcgaccgtg aacgcgaccg gcatcctgtt gcgcgcggtg
     2581 gtctccgatc ccgatctggg cgactcgctc caactcgagg gggaggtccg gcccactgga
     2641 atcgcgttca ccggtcccaa cgtgcccgac gggccggtgg ccggcaacgg gcaaaccacg
     2701 tttctgagcg tcggcgggct gatcgacggg acgacctatc attggcgcgt gcgtgcgcgg
     2761 gacaacaccg ggcgctcggg tccgtgggtg gcgttcggcg gcaatcccga cttcgtggtg
     2821 aacgtgctgc acccgcccaa cgcgccgacg accctgggcc aggccacggc gggcggcgcc
     2881 gtcctgctga ccggcgccac gaccgacacg aacatggtgc gcctcggcgc cgtggtgtcg
     2941 gaccccgacg cgggcgatca gttgcggctg gaggtggagg tgcggcccgt gggtgtgccg
     3001 ttcacggcgc cgacgaacgc gagcgctcca gccgtggatg gcggcgcgct gcaggtactg
     3061 gtggggccgc tgccgggcgc cacgagctat cactggcgcg cgcgggcggt ggaccagacg
     3121 ggcgacacca gcagctgggt ggcctatggc gggaacgcgg agagtgcggc ggatttcacc
     3181 atcgcggcgc agcagcacgc ggcgtacccg ccgacgtctc tggcgcaact gcggagcagc
     3241 gacggctcgc ccatcccggt cggcggcgcc gtcgcgtcga ccagcatcgt cctcgccgcg
     3301 gtcgtgagcg atctggatcc cacccggacc ctgcagctcg aggtcgaagt ccagccggtg
     3361 ggacagggct tcctcggccg gcccacgtcc tcgagcgcgt tcgtcgcgag cggtacggtc
     3421 gcgtcggtga gcgtcgggtc gttgaccgcc agcaccgggt accactggca agcgcgggcc
     3481 cgggacgacg gggccgacac cagtgcctgg gtgtcgttcc ccgaggggcc cggcaacgcc
     3541 gagaccgatc ccgatttcgt cgtgcagacg ccgccggtgc ggctcgtgtt tacggtgcag
     3601 ccgagcaatg ccgtggtcgg gacggcgatc atcccggcga tcgaggtcac ggcgttggac
     3661 gccaacggcc aggtcgtgac ggcctacagc gacagcgtgg ccatgaccat cgcgacgaac
     3721 ccgagcggcg gcaggctctc ggggacgacg accgtcgtcg cggtggcggg cgtggcgagg
     3781 ttcgcggacc tgaagatcaa caaagccgga acgggctaca cgctgcaggc cgccacgaga
     3841 tctcccgcgc tcagcgtcat cagcgtcccg ttcgacatca cgccgaagac gaaaccctag
     3901 cggggccggt gccggactca ggagacgacc tggtcctcgg ccgcgggcgg cgtgaaatag
     3961 ccggccagcg ccaccaacac cgtcgtgacc gcccctgcga cctccgggcc gatctgctgc
     4021 gccgtcg
//
LOCUS       DBNGZW010000373         3952 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_513551, whole genome shotgun sequence.
ACCESSION   DBNGZW010000373 DBNGZW010000000
VERSION     DBNGZW010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3952)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3952)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3952
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_513551"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..997)
                     /locus_tag="AC16JC_08745"
     CDS             complement(<1..997)
                     /locus_tag="AC16JC_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR5164823.1"
                     /translation="MIRPERLTIKAAEALQQAASHARARGNPVVNDAHLFLALLQQDE
                     GIVTPLLQKAGLNAAQLRAETEREIERFPSQSGGAVEPTLSRELSAVLDRADKVAKGL
                     GDAYVSTEHLLLALAEEKGATARQLLTAAGVNQADLLSALEGVRGAHRVTDQQPEEKY
                     QALERFTRDLTEAARAGKLDPVVGRDEEIRRVMQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVNGDVPESLRNKHLVSLDLGALIAGTKYRGEFEERLKSVIKEITTAEGRYV
                     VFIDELHTLVGAGAAEGAVDASNLLKPALARGELHVVGATTLDEYRKHIEKDAALE"
     gene            complement(1202..1645)
                     /locus_tag="AC16JC_08750"
     CDS             complement(1202..1645)
                     /locus_tag="AC16JC_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HIR5164824.1"
                     /translation="MKRHAWALVGLGLLAVGPGSRDMSGQQGTQSATVQLRQNFPNPF
                     NPATTIPFSLGTEMFSSGHRPKVSLKVYNVLAQLVAVPILQGTGERLDNVELSCATPV
                     GDACDYSAYWDGNVLNTGQQAASGIYIYQLVVDGRRFTKKMIVMK"
     gene            complement(1729..3630)
                     /gene="selB"
                     /locus_tag="AC16JC_08755"
     CDS             complement(1729..3630)
                     /gene="selB"
                     /locus_tag="AC16JC_08755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HIR5164825.1"
                     /translation="MAPFHRFVVGTAGHIDHGKTALVKALTGVDTDRWEEEKRRGITI
                     DLGFAPIDLGPGLEASVVDVPGHEGFVRNMLAGATGIDAALLVVAADEGIMPQTAEHL
                     AIVELLGVRRGIPVLTKRDLVDDDWLALVETELAARLAGSPVRWDPPIATSAVSGVGL
                     DALREALRRLSADLPGRSEDDLFRLPIDRVFALPGAGTVVTGSTWSGTVAVGDAVRLL
                     PLDREVRVRSIEVHGKTADRAVPGRRTALALVGVDRHELARGDVVVTGEGWHSTERLD
                     ADVELLPGASRPVLSRTRLRVHLGTAEVLARASQTGPIAPGERGRVRLVLESPLVARG
                     GDRFVVRSFSPVTTNGGGVVLDPLPPAEPRLPGRRLTQSQTAAERLAAWVEEAGLAGL
                     RPGDLPVRLGIPMKDVSGVIAAAGKGVLVCGEWLTARSAAAAEAERLGVLVGAHHDHH
                     PLDPGMSLQALRATPGLRKVPEPIVDTVIDLGVRKRLLEVSGGVVRRPGWSASLDRGA
                     SDARSAIAARIIAAQWQIPTVAELEREFAGTPVRALLAHLAREGGVEQVDQERYAAPA
                     ALAGFRSRLEAVLGELGEATPAELRTHLGLTRKYLIPLLEWADRHGVTERRGDARVLA
                     RLTGRSKGT"
     gene            complement(3606..>3952)
                     /locus_tag="AC16JC_08760"
     CDS             complement(3606..>3952)
                     /locus_tag="AC16JC_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164826.1"
                     /translation="SLRACVRDSAAVGEGGEGVPVAFSTAEGGVLCEACLRSRPPATL
                     TRLPWDAWRDLRAMLDPRAPLPRLDAPHAASHRRLAARFVRHHLEDSGTAESLPALVF
                     WERRAWPPSIAS"
BASE COUNT          634 a         1492 c         1248 g          578 t
ORIGIN      
        1 gctccagcgc cgcgtccttc tcgatgtgct tgcggtactc gtcgagcgtc gtggcgccga
       61 cgacgtgcag ctcgccgcgc gccagcgccg gcttcagcag attggacgcg tccaccgccc
      121 cctcggccgc ccccgcaccg accagggtgt gcagctcgtc gatgaacacg acataccgcc
      181 cttccgccgt cgtgatctcc ttgatcaccg acttgaggcg ctcctcgaat tcgccgcgat
      241 acttggtacc cgcgatcagc gcgcccaggt cgagcgagac gagatgcttg ttgcgcagcg
      301 actccggaac gtcaccgttc acgatccgct gggccagccc ctcgacgatt gccgtcttgc
      361 ccacccccgg ctcgccgatc agcaccggat tgttcttggt gcggcgcgac agcacctgca
      421 tcacacgccg gatctcctcg tcccggccca ccacggggtc gagcttgccg gcgcgggccg
      481 cctcggtgag gtcccgcgtg aaccgctcca gcgcctggta tttctcctcc ggctgctggt
      541 cggtgacacg gtgcgcgccc cgcactccct cgagcgccga gagcagatcc gcctggttca
      601 cccccgccgc cgtgaggagc tggcgtgccg tggcaccctt ctcctcggcc agagcgagca
      661 acaggtgctc ggtcgagacg taggcgtcgc ccaagccctt cgccaccttg tcggcacgat
      721 cgagcaccgc cgacaattcg cgggacaagg tcggctcgac cgcaccgccg gactgactgg
      781 ggaaccgctc gatctcccgc tcggtctcgg cgcgcagctg agcggcgttg agccccgcct
      841 tctggagcag tggcgtgacg atcccctcgt cctgctgcaa gagggcaaga aacagatgcg
      901 cgtcgttcac cacgggattg ccgcgggcgc gcgcatggga tgccgcctgc tggagcgcct
      961 cggcggcctt gatggtgagt cgttctggtc tgatcatggc agggagaccc ctagcaaccc
     1021 gtgtaccgga taaacgcgcc aaagtgacag gcgcaaggcg aaaaatgcgt caagaagaga
     1081 cgtcgagcaa agacgtcgag caaagacgtc aagcaaaaac gtcaagcgga accgctcttc
     1141 acgattccgc ttgacgtatg cgctcgaggc cgtcgctcga cggttctgct cgacgtcttg
     1201 cttatttcat gacgatcatc ttcttcgtaa atcgccgtcc atccaccacc agctggtaga
     1261 tgtagatccc cgaggccgcc tgctggcccg tattcaggac gttgccgtcc cagtacgcgc
     1321 tgtagtcgca ggcatccccg accggcgtgg cgcacgacaa ctctacgttg tccagcctct
     1381 cgccggtgcc ctggagaatc ggcacggcaa ccagctgcgc caacacgttg tagaccttca
     1441 acgaaacctt cggccggtga ccactggaaa acatttccgt tccgagcgaa aacggaatcg
     1501 tggtcgccgg gttaaagggg ttcgggaaat tctgcctcaa ctgcaccgtg gcggattgag
     1561 taccctgctg tcccgacatg tctcgagacc ccggacccac cgccaacagc ccgagcccca
     1621 ccaacgccca ggcgtgccgc ttcatcacca ctcctcgatc gaacgtaaaa gagcgattac
     1681 ccatctcctt gagttacggg gaaccctgct gcagcctgcg attccaacct aggtcccctt
     1741 gctccgacct gtcaagcgag ccagcacccg ggcgtccccc cgacgctcgg taactccatg
     1801 ccggtcggcc cattcgagca acggaatcag atactttcgg gtcaggccca ggtgggtacg
     1861 caactcggcg ggcgtcgcct cgccaagctc ccccagcacc gcttccagcc tgctccgaaa
     1921 cccggccagt gccgcggggg cggcatagcg ctcctggtcg acctgttcta caccgccttc
     1981 ccgggcgagg tgcgcgagca gcgcccgcac cggcgttccc gcgaattccc gctccaactc
     2041 cgccacggtc gggatctgcc actgcgccgc aatgatgcgg gccgcgatcg cgctccgggc
     2101 gtcgctggcc ccgcggtcca gcgatgcgct ccaacccggg cgtcgcacca ctcctcccga
     2161 cacctccagc agccgtttgc gaacgccgag atcgatcacc gtatcgacga tcggctccgg
     2221 caccttgcgg aggcccggcg tggcgcgcag cgcctggagg gacatacccg ggtcgagtgg
     2281 gtggtggtcg tgatgcgccc ccaccagcac gcccagccgc tccgcctccg ccgcagcggc
     2341 cgaccgcgca gtcagccact cgccgcagac taacacaccc ttgcctgccg cggcgatgac
     2401 gccgctcaca tccttcatag ggatgccgag ccgtaccggc aggtcgcctg gacgcagccc
     2461 ggccagcccg gcttcctcca cccacgccgc gaggcgctcg gcggcggtct gcgactgggt
     2521 gagccggcgc ccggggagcc ggggctcggc gggaggaagc ggatccagca cgacgccgcc
     2581 gccgttcgtg gtcaccggcg agaagctgcg caccacgaag cggtcgccgc cccgagcgac
     2641 cagcggcgac tcgagcacca gccgcacccg gccccgctcg ccgggggcga tcggaccggt
     2701 ctgcgacgcc cgtgccagca cctccgccgt gccgagatga acccgcagcc tcgtgcgcga
     2761 gagcaccggc cgcgacgcac ccgggagcag ctcgacgtcc gcgtccaggc gctcggtgct
     2821 gtgccatccc tcgcccgtca ccaccacatc cccgcgtgcc agctcgtgac gatccacgcc
     2881 caccagcgcc agcgccgtgc gacgtcccgg cacggcccgg tccgcggttt tcccgtgaac
     2941 ctcgatggag cgaacgcgca cctcgcgatc cagaggcagc agccgaacgg catcccccac
     3001 ggcgaccgtc ccgctccagg tcgacccggt cacgaccgtg ccggcccccg ggagggcgaa
     3061 tacgcggtcg atcggcagcc ggaacaggtc gtcctcggag cgtcccggca gatctgcgct
     3121 gaggcggcgc agcgcctcac gcagcgcgtc gagccccaca ccgctcacgg cggacgtggc
     3181 gatgggcggg tcccagcgca ccgggctgcc ggcgagccgc gccgcgagct cggtctcgac
     3241 caacgccagc cagtcgtcgt ccaccaggtc gcgcttggtg agcacgggga tgccgcgccg
     3301 cacacccagc agctccacaa tggcgaggtg ctccgcggtc tgcggcatga tcccctcgtc
     3361 ggccgccacg accagcagcg ccgcatcgat cccggtcgcg ccggcgagca tgttgcgcac
     3421 gaacccctcg tgacccggga cgtccaccac gctcgcctcg agaccgggcc cgaggtcgat
     3481 cggggcgaaa cccaggtcga tcgtgatgcc gcgtcgcttc tcctcttccc agcggtcggt
     3541 gtccaccccc gtcagtgcct tgacgagcgc ggtcttcccg tggtcgatgt ggccggccgt
     3601 gccgactacg aagcgatgga agggggccac gcgcgccgct cccagaagac gagcgcgggg
     3661 aggctctcag ccgtccccga atcctcgagg tgatgccgga cgaagcgggc cgcgaggcgc
     3721 cggtgcgaag cggcgtgcgg cgcatcgagc cgcggcaggg gcgcgcgcgg gtcgagcatg
     3781 gcgcggagat cccgccacgc gtcccacggc aaccgggtga gcgtggcagg gggccgcgac
     3841 cgcaggcagg cttcgcacaa tacgccgccc tcggcggtgc tgaacgccac cggtacaccc
     3901 tccccgccct ccccgaccgc cgcggagtcc cgcacgcagg cgcggagcga cg
//
LOCUS       DBNGZW010000374         5872 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_239094, whole genome shotgun sequence.
ACCESSION   DBNGZW010000374 DBNGZW010000000
VERSION     DBNGZW010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5872
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_239094"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..1247
                     /locus_tag="AC16JC_08765"
     CDS             63..1247
                     /locus_tag="AC16JC_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR5164827.1"
                     /translation="MGRIRRYLVGVALGTAVLAAAFVPLPVLVDAVSGSPAGDAELSL
                     PLSYVVTAPVSNLLDALTQLSMDRAVSLLIVWVVALLLIGVVRRGSRTRRIVRALVGA
                     VIPFVLAVAAVFLPRPVPRLVTSESDVNVLDFHSHTAASHDGRHGWTPERVASWHAAQ
                     GFDAAYITDHNIPFDGSNDAAIPLLPGVEWSVYRQHIVALGGLPLTDLAPYSHDTPGL
                     LGLFAALHARGSIAVASLPEYWRNHWEDLDQFVSAGLDGFEIVNCAPKALEFPSADRQ
                     AVIALAAQHDLLVDGASDNHGWGTVTCVWNLAREGSHGFRDNQVVARPLALAQGDLPA
                     WDAAYSQPWLMLRALSWPERMSWLTWIALLTIWRGIPRRRGQPRGLGILARSVGWRRS
                     EV"
     gene            1297..>5872
                     /locus_tag="AC16JC_08770"
     CDS             1297..>5872
                     /locus_tag="AC16JC_08770"
                     /inference="COORDINATES: protein motif:HMM:NF019323.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HIR5164828.1"
                     /translation="MLSAPLRLVLAAALGATALSTTDELKVLRVSPQNTGEATEDVTV
                     TFDRPVAGGLDSTVDPRTIFQITPRVPGRLEWRDPVTIRLHPAAPLPAGVTFTVRIAN
                     TFQAMDGSRLAAPYAFSFRVKGPRVLDAYPVNQWTNPKYLTPDVHFTLLLSAPANLAL
                     LAPLASVDVGQCVRGKTIPLRPVRQRRIGKTDERWRYYGDYVEDSTRDTRRVVELAPA
                     GPLPLNCAATLRIPERVDSVTTIRSWPFHTYGPLAVDTVLCAWDTGRCPIGPVHVWFT
                     TPVRGSEVLRHVHVSPGITFTVNDSTGESDNWFLDAQLTPRTRYLVTVDSGLTDVFAQ
                     RLTARFAQSLATTSYTPQVSYPYGRMVVERQGPRTLAVQHVNADTLVITIVGVPDSLE
                     RRILGNQGAWGDVWEKLKSQATVTTVAVPGVRDVPRASAVRIPALNASRPGAATLQMI
                     RISSHALDTLPRNDGSPFTIAVVQLTDLAVHAKIGAEEGTVWVTGVSDGTPRSGARVT
                     LYDNDGKVRATATTDARGLARLTGYAGDTTQGAGDAAEECEYCDYSGFEGYVAATLGT
                     DRSLVSVSRYDPDLSPWQFNVSAAYGPERLERAAAIFTERGIYRPGEPVYAKAIVRDG
                     QLGSLRVPRGDSLRWVFTDRENATVRDTTVALSAFGTADQSYAIPPDAPLGYYTVRVD
                     RKSDGKWRSGTTTNYRVAEYRPPEFLVTATTDRVPRFAGDTVRASVEARYLFGAPMAR
                     AAVTWYARQRTAGPWEYDIPNTEGFMLGEMGWWWEEEQDESPGTAAVLASGTDTLDAT
                     GHLTLAAAAPVPPKGKAAWVTLEATVTDVNRQTVAAQASALVHPASIYIGAKPTGTDY
                     FWTAGKERSVSLIAVTPEGVRTPGVAIRATLIHREWHRVRRDRDGAVEDVGEWVSDTV
                     GRCAVTTANEPVNCSFTPADGGSYTLRFTAADPKGRPVATSFYRWVVGKDWVPWNDAS
                     QFKMDVVPDKSRYNVGDTATVLFASPFTNAEAWITVEREGVIEERRQRITSGTTVLKL
                     PITEALVPNAFVSILVARGRSAPPQAPDDPGRPTIRVGYAQIQVTPAVKRLTVDVKAL
                     AEEYRPGDTARVDVRVRDAAGAGRRAEVTLWAVDEGVLSLTGYRTPDPVDLIYQPRGL
                     GLQLASSLTSVAAQITEGEKGQRAPGGGGGMDVEGILRSRFQTTAFFLGSVVTDTAGH
                     ALVRAKLPDNLTTFRLMAVAVTAGDRYGSGEAKLLVTRPLLARPALPRFLREGDSLLA
                     GVVVNSRLGGTPTVTVQAQAQGAALLGPATKAATLEAGRGREVRFTFRGTAGDTAALR
                     FKVSSGVNADAVETRLPIELPYRPRAFEVAGTLVDSTSAEFVFPEAIDPGRSRLVLSY
                     GASPLALIRGYRETLHVYPYYCTEQTASVALPLIALYRAQKQLGGAPLLKGNPAAEIG
                     QAVAMITGRQRDDGAIGYWSRGDWSSPWLSAYAGRTLLAARAAGVAVNDSVVQRLAGY
                     LSTWLHNPQRLMIPVYHWYGDQKVFLGERVA"
BASE COUNT          831 a         2155 c         2016 g          870 t
ORIGIN      
        1 ggtcgaccgg aagcgctcga tcccgtactt tgtgggcgac cgtccatgca cggattctag
       61 aggtggggag gatacgccgc tacctcgtgg gggtggcgtt ggggaccgcg gtgctcgccg
      121 cggcgttcgt accgcttcca gtgttggtgg acgcggtgag cggctctccc gcgggtgacg
      181 cggagctgtc gctgccgttg tcctacgtcg tgacggctcc ggtctccaat ctgctcgacg
      241 cgctcaccca gctcagcatg gaccgggcgg tctccctgct catcgtctgg gtcgtggcgc
      301 tcctcctgat cggcgtagtg cgtcgcggca gccgcacccg gaggatcgtg cgggcgttgg
      361 tgggagccgt gatccccttc gtcctggccg tcgcggccgt gtttctcccc cgccccgtcc
      421 cgcggctcgt gaccagcgag tcggacgtca acgtgctgga tttccattcc catacggccg
      481 cctcgcacga cgggcgccac ggctggaccc ccgagcgggt ggcgtcgtgg cacgccgccc
      541 aggggttcga cgcggcgtac atcaccgacc acaacatccc cttcgacggc tcgaacgacg
      601 cggcgattcc attgctgccc ggcgtcgaat ggagcgtgta ccgccagcac atcgtggccc
      661 tcggcggcct gccgctgacc gacctcgccc cctattccca cgacaccccg ggactgctcg
      721 gcctgtttgc cgcgctccac gcgcggggga gcatcgccgt cgcctcgctt cccgagtact
      781 ggcgcaacca ctgggaagat ctcgaccagt tcgtgtcggc ggggctggac gggttcgaga
      841 tcgtcaactg cgcccccaag gcgctcgaat tcccctcggc cgatcgccaa gcggtgatcg
      901 cgctggccgc ccagcacgac ctgctggtcg acggggccag cgacaaccac gggtggggca
      961 ccgtcacgtg cgtgtggaat ctggcgcggg agggctcgca cgggttccgc gacaaccagg
     1021 tcgtcgcgcg cccactggcg ctcgcccagg gcgatctgcc ggcctgggac gcggcctatt
     1081 cgcagccctg gctgatgttg cgggcgctgt cgtggcccga gcgcatgagc tggctgacat
     1141 ggatcgcgct gctcaccatc tggcgtggca tcccgcgacg gaggggacag ccgcgcggcc
     1201 tggggatcct ggcccggtcg gtcgggtggc ggcgctcgga ggtttgacac cgcggaacga
     1261 cgtgcttaga tgcaataatc ctcaacgggt acgccgatgc tctccgcccc gcttcgcctc
     1321 gtcctcgccg ccgccctcgg cgccaccgcc ctctccacca ccgacgagct gaaggtgctg
     1381 cgcgtctcgc cccagaacac gggtgaggcc accgaggacg tcactgtcac tttcgaccgc
     1441 cccgtggcgg gcggactcga ctcgacggta gacccgcgca ccatcttcca gatcaccccg
     1501 cgggtgccgg gcaggctcga gtggcgcgac ccggtgacca tccgcctcca ccccgcggcc
     1561 ccgctgccgg ccggcgtcac cttcacggtc cgcatcgcga acaccttcca ggcgatggac
     1621 ggcagccggc tcgcggcgcc ctacgccttc tcgttccgcg tgaaagggcc gcgcgtcctc
     1681 gacgcgtatc cggtcaacca gtggaccaac ccgaagtacc tgacaccgga tgtccatttc
     1741 acgctgctcc tgtcggcacc ggccaatctc gcgctgttgg cgccgctcgc gtccgtggac
     1801 gtgggccagt gcgtccgcgg gaagacgatc ccgctgcggc cggtgcggca gcgccgcatc
     1861 ggcaagacgg acgagcgctg gcgctactac ggtgactacg tcgaggactc gactcgcgac
     1921 acccgccgcg tggtggagct cgcccccgcc gggccgctgc ccttgaactg cgcggccacg
     1981 ctgcgcatcc ccgagcgcgt ggacagcgtc accacgatcc gctcctggcc gttccatacc
     2041 tacgggccgc tggcggtgga cacggtgctc tgcgcctggg acaccgggcg ctgtccgatc
     2101 ggcccggtgc atgtctggtt caccacgccg gtgcgcggca gcgaagtgct gcgccacgtc
     2161 catgtgtccc cgggcatcac gttcaccgtg aacgactcga ccggcgagtc cgacaactgg
     2221 ttcctcgacg cccagctcac cccgcgcacc cgctacctcg tcacggtgga cagcggcctg
     2281 accgacgtgt tcgcgcagcg gctcaccgca cgcttcgcgc agagtctcgc caccacgtcc
     2341 tatacgccgc aggtcagcta cccctacgga cggatggtgg tggagcggca gggcccacgc
     2401 acactcgccg tgcagcacgt gaacgcggac accctggtga tcacgatcgt cggcgtcccc
     2461 gactcgctcg agcgccgcat cctggggaat cagggcgcct ggggagacgt gtgggagaag
     2521 ctgaagagcc aggccaccgt gacgaccgtc gccgtcccgg gcgtccgcga cgtaccgcgc
     2581 gcctcggccg tacgcattcc ggcgctcaac gcctcgcgcc ccggcgccgc cacgctccag
     2641 atgatccgca tctcgagcca cgccctcgac acgctgccgc gcaacgacgg ctcgccgttc
     2701 accatcgcgg tcgtgcagct caccgatctc gcggtccacg cgaagatcgg cgccgaggaa
     2761 ggcaccgtgt gggtcaccgg ggtgagcgac gggacgccgc gctcgggagc gagggtcacg
     2821 ctctacgaca acgacgggaa ggtccgcgcc acggcgacga ccgatgcccg cggcctcgcc
     2881 cggttgacgg ggtacgccgg cgacacgacg cagggcgcgg gggacgcagc cgaagagtgc
     2941 gaatactgcg actactcggg gttcgagggc tacgtcgcgg cgacgctcgg caccgaccgc
     3001 tccctggtga gcgtttcccg gtacgacccc gacctttctc cctggcagtt caacgtctcc
     3061 gccgcctatg gaccggagcg tctggagcgg gccgcggcga tcttcaccga acgcggcatc
     3121 taccgccccg gcgagccggt gtacgccaag gcgatcgtac gcgacggcca gctgggcagc
     3181 ctgagggtgc cgcgcggcga ttcgctccgc tgggtcttca ccgaccgcga gaacgccacg
     3241 gtgcgcgaca ccacggtcgc actctcggcg ttcgggaccg cggaccagtc ctacgccatc
     3301 cccccggacg caccgctcgg ctactacacg gtgcgcgtgg atcgcaagag cgacggcaaa
     3361 tggcgctccg gcaccaccac gaactaccgc gtcgccgagt accggccgcc ggaattcctg
     3421 gtgaccgcca ccacggaccg cgtcccccgc ttcgcgggag acaccgtccg cgcgagcgtc
     3481 gaggcccgtt acctcttcgg cgcgccgatg gcccgcgccg ccgtcacctg gtacgcgcgg
     3541 cagcgcaccg ccggcccctg ggagtacgac atccccaaca ccgaaggctt catgctcggc
     3601 gagatgggat ggtggtggga ggaagaacag gacgagagcc cgggcaccgc cgccgtgctc
     3661 gccagcggca ccgacacgct cgatgcgacc gggcacctca cgctcgctgc ggccgctccg
     3721 gtgccgccca agggcaaagc tgcctgggtg accctcgagg ccaccgtcac cgacgtgaac
     3781 cgccagaccg tggcggcgca ggcgtcggcg ctggtccatc cggcttcgat ctacatcggg
     3841 gcgaagccca ccggcaccga ctacttctgg accgccggga aggagcgcag cgtcagcctc
     3901 atcgccgtga cgcccgaagg cgtgcgcacg ccgggcgtgg cgatccgcgc cacgctgatc
     3961 caccgcgaat ggcaccgcgt gcgccgcgac cgcgatggcg cggtcgagga cgtgggcgag
     4021 tgggtgtcgg acacggtcgg ccgctgcgcg gtgacgaccg caaacgagcc ggtgaattgc
     4081 agcttcacgc ccgcggacgg cggctcctac acgctccgct tcaccgccgc cgacccgaag
     4141 ggccgccccg tcgccacgag cttctatcgc tgggtcgtgg gcaaggactg ggtgccgtgg
     4201 aacgacgcct cgcagttcaa gatggacgtc gtccccgaca agagccgcta caacgtgggc
     4261 gacaccgcca cagtcctgtt cgcctctccg ttcaccaacg ccgaggcctg gatcacggtc
     4321 gagcgcgagg gcgtgatcga ggagcggcgg cagcgcatca cctcgggcac caccgtcctc
     4381 aagctcccaa tcaccgaggc gctggtcccc aacgcgttcg tttcgattct cgtcgcgcgt
     4441 ggccgcagcg ccccgccgca ggcgcccgat gatcccggcc gccccacgat ccgcgtgggg
     4501 tacgcccaga tccaggtcac cccggccgtg aagcgtctga cggtcgacgt gaaggcgttg
     4561 gccgaggaat accggcccgg cgataccgcc cgcgtggacg tgcgcgtgcg ggatgccgcc
     4621 ggcgcggggc gccgcgcgga ggtgaccctg tgggccgtgg acgaaggggt cctgtcgttg
     4681 accggatacc ggaccccgga tcccgtcgat ttgatctatc agccgcgcgg gctcgggctg
     4741 cagctcgcca gctcgctcac ctcggtcgcg gcccagatca ccgagggcga gaaaggacag
     4801 cgcgcgcccg gcggcggcgg cggcatggac gtcgagggga ttctccggtc ccgcttccag
     4861 acgaccgcgt tcttcctggg gtcggtggtg accgacacgg ccggccacgc gctcgtccgg
     4921 gcgaagctcc ccgacaacct caccaccttc cgtctgatgg cggtggccgt caccgcgggc
     4981 gaccgctacg gctccggtga ggccaagctg ctcgtcacgc ggccgctgct cgcgcggccg
     5041 gcgctgccgc gcttcctgcg tgagggtgac tccctcctgg cgggggtcgt ggtgaacagc
     5101 cggctcgggg gcacgccgac ggtgaccgtc caggcgcagg ctcagggcgc cgcgctgctc
     5161 ggcccggcca cgaaggcggc cacgctcgaa gcgggccgcg gtcgcgaggt ccgcttcacc
     5221 ttccgcggca cggccggcga tacggcggcg ctccgcttca aggtgtcgag cggcgtgaac
     5281 gcggacgccg tggagacccg cctgccgatc gagctgccct accggccgcg cgcgttcgag
     5341 gtggcgggga cgctggtcga ttccacgtcg gccgagttcg tcttccccga ggcgatcgat
     5401 ccggggcggt cgcgcctcgt gctgtcctac ggggcgtcgc cgctcgcgct gatccgcggc
     5461 tatcgggaga cgctccacgt gtatccctac tactgcaccg agcagacggc gagcgtcgcg
     5521 ctgccgctca tcgcgctgta ccgcgcccag aagcagctcg gcggtgcgcc gctgctcaag
     5581 ggcaaccccg cggcagagat cggccaggcg gtggcgatga tcaccggccg ccagcgggac
     5641 gacggcgcca tcggttactg gagccgcggc gattggtcgt ctccgtggct gtcggcgtat
     5701 gcggggcgga cgttgctcgc cgcgcgcgcg gcgggcgtgg cggtgaacga ttcggtggtg
     5761 cagcggctcg ccgggtatct ctcgacgtgg ctgcataacc cgcagcggct gatgatcccc
     5821 gtgtaccact ggtacggcga ccagaaggtc ttcctgggcg agcgggtcgc cg
//
LOCUS       DBNGZW010000375         2856 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1534998, whole genome shotgun sequence.
ACCESSION   DBNGZW010000375 DBNGZW010000000
VERSION     DBNGZW010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2856)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2856)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2856
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1534998"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="AC16JC_08775"
     CDS             complement(<1..638)
                     /locus_tag="AC16JC_08775"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5164829.1"
                     /translation="MTEGLSPNLQHLETSATIAISQEAKRRRAAGEDVIDLGAGEPDF
                     PTPPIPAEAGIRAIREGKTRYPTNEGILELRAAAAKHLALLSGGRPINADHIVVSTGS
                     KQSLFNACFSLFGQGDVVAIPAPAWVSYPQIVHLARARPVLVAGDPEWGLKVSVRELE
                     RILPAPKGLILCSPCNPTGSVYALAELRAIAEWAAARKVWIIADEIYRRIHYG"
     gene            787..1539
                     /locus_tag="AC16JC_08780"
     CDS             787..1539
                     /locus_tag="AC16JC_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR5164830.1"
                     /translation="MTAIPRIEPSGPSAAPRQPALRRRGMSRRTEGRSQPGSRLNSRE
                     AACLEVVCRLAELIESSDHYTFGHCARVAAYSVDVARSLEFNDHEITTVRIGAYLHDL
                     GKVRVPAEILNKPGPLEAEESDLIRWHPLWGVELLAAIDLPWDIKPIIRWHHERYDGS
                     GYPHGLRGDEIPITAQIVGIADAFDALTTTRSYRPAVSIEQAIVEMRANRHQWHPDVY
                     RAFLGTVTAQQAQWRTIPWRPRIATIESIGSA"
     gene            complement(1476..2561)
                     /locus_tag="AC16JC_08785"
     CDS             complement(1476..2561)
                     /locus_tag="AC16JC_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HIR5164831.1"
                     /translation="MGLRIGVLPPGPLDAITDVAGVRVGQVTLARGDSINTGVTAILP
                     HAGNVFRQKVPAAIVIGNAFGKLAGSTQVNELGELESPIVLTCTLCVPRAADAVLTYL
                     LGLPGNEDVRSANPVVAETNDGYLNDIRSRPITEADVLTAIHSAASGPVAEGAVGAGR
                     GTVAFGWKGGIGTASRVVPGRLGGWTVGVLAQTNFGGVLTIDGAPVGQELGQYYLKGD
                     LGDGSVIMVVATDAPLDHRQLERLGRRALAGLARTGSSMTNGSGDYVIAFAVGRSSGQ
                     AVGPSGEPPTARPPVRPTARLTDTVEVATNDAMSPLFEAVIEATEEAIVNSLLRAETV
                     KGFRGTAYALPIDSIVAILGRHGMVRH"
BASE COUNT          456 a         1019 c          936 g          445 t
ORIGIN      
        1 ccgtagtgga tacggcggta gatctcgtcg gcgatgatcc acaccttccg cgccgccgcc
       61 cactccgcga tggcgcgcag ctcggccagc gcgtagaccg accccgtcgg gttgcacggc
      121 gagcacagga tcaatccctt cggcgcgggc agaatgcgct ccagctcccg cacgctcacc
      181 ttcaaccccc actccggatc tccggcgacc agcaccggac gggcccgtgc caggtggacg
      241 atctgcgggt acgacaccca ggccggggcg gggatcgcca ccacgtcgcc ctgaccgaac
      301 agcgagaagc aggcgttgaa cagcgactgc ttcgacccgg tggagacgac gatatgatcg
      361 gcgttgatcg ggcggccgcc cgagagcagc gccaggtgct tggccgccgc ggcccgcagc
      421 tccaggatgc cctcgttggt cggataccgc gtcttccctt ccctgatcgc ccggatgccg
      481 gcctcggccg ggatcggcgg agtgggaaag tcgggctcac ccgcccccag atcgatcacg
      541 tcttcgcccg cggcccggcg acgcttggcc tcctgcgaaa tcgcgatggt ggctgaggtc
      601 tcgaggtgct ggaggttggg ggacaaccct tcagtcatgg cgtaaagctc cgtggcgtaa
      661 tgggatagca acggggcgct ggaccccggg cacgttagcc ccggggtgtc catcggcgca
      721 acggggcggg ggtgcggcaa acctgctgcg tgaccaccgc ggggtgttgc gcatcttcac
      781 caagccgtga ccgcaatccc tcgtatcgag ccatcgggcc cctctgcggc cccccgccag
      841 ccggcgctgc gccggcgggg tatgtcgcgc cgcaccgagg ggcggtcgca gcccgggtcg
      901 cgactcaact cgcgtgaggc agcctgtctc gaagtcgtct gccgcctggc cgagctcatc
      961 gaatcgagcg accactacac gttcggacat tgtgcgcggg tggcggccta ctccgtcgac
     1021 gtggcccgga gccttgagtt caacgatcac gagatcacga ccgtgcgtat cggcgcgtac
     1081 ctgcacgacc tgggaaaagt acgcgtgccg gccgagatcc tgaacaagcc gggcccgctc
     1141 gaagccgagg aaagcgacct catccggtgg caccccctgt ggggcgtcga gctgctcgcc
     1201 gcaatcgacc tgccctggga catcaagccc atcatccgct ggcaccacga gcgctatgac
     1261 ggttcggggt acccgcacgg cctgcgcggc gacgagatcc cgatcaccgc ccagatcgtc
     1321 gggatcgccg acgcgttcga cgccctcacc accacgcgca gctaccggcc ggcggtgtcg
     1381 atcgagcagg cgattgtcga gatgcgcgcc aaccggcacc agtggcaccc ggatgtctac
     1441 cgggcgttcc ttggcaccgt caccgcacag caggctcagt ggcgcaccat cccgtggcgc
     1501 cccaggatcg cgacgatcga atcgatcggc agcgcgtaag cggttccacg gaatcccttc
     1561 acggtctccg cccgcagtaa cgaattcaca atcgcttcct cggtcgcctc gatcaccgcc
     1621 tcgaatagcg gggacatcgc gtcgttggtg gcgacttcaa cagtgtcggt caggcgggcg
     1681 gttgggcgga caggcggtcg ggcggtcggt ggctccccgg atggaccgac cgcctgaccg
     1741 cttgaccgcc cgaccgcaaa cgcgatcacg tagtccccgc tgccgttggt catgctgctc
     1801 ccggtgcgtg cgaggcccgc gagcgcgcgt ctgcccagtc gctcgagctg gcggtggtcg
     1861 agcggcgcat cggtcgccac gaccatgatt acgctgccgt cgccgagatc acccttcaga
     1921 tagtactggc cgagctcctg cccgacgggc gcgccgtcga tcgtcagcac cccgccgaag
     1981 ttcgtctggg ccagcacgcc gacggtccag cctcccagcc gccccgggac cacccggctg
     2041 gccgtcccga tcccgccctt ccagccgaac gccaccgtgc cgcgccccgc acccaccgcc
     2101 ccttcggcga ccgggccgga tgccgccgag tggatcgccg tgagcacgtc cgcctcggtg
     2161 atcggtcgcg accggatgtc gttcaggtat ccgtcgttcg tttcggccac caccgggttc
     2221 gccgagcgca cgtcttcgtt gcccggcagg ccgaggaggt aggtgaggac ggcgtcggcc
     2281 gcccgcggca cgcagagcgt gcaggtcagg acgatcggcg actcgagctc gcccagctcg
     2341 ttcacctggg tggagccagc gagcttgccg aatgcgttcc cgatcacgat cgccgcgggg
     2401 actttctgcc ggaacacgtt tcccgcatgc ggcaggatcg cggtgacccc ggtgttgatc
     2461 gaatcgccgc gcgccagcgt gacctgcccc acgcgcaccc ccgccacgtc ggtgatggca
     2521 tcgagcggac ccggcggcag gacgccgata cgcagtccca actcgggggc acggggacgc
     2581 tgttgggcgc cagctgacgc agccagggat gacaggagcc acacaatgtg gaatgcggaa
     2641 tgcgaaatgc ggaatcgaac ggtgagctcc gtcctcgaca ttcgcgattt cgctccacat
     2701 tcgacgttcc gcattccgca ttctccggaa gccgttgcca agcgatttcc gattgcgcgt
     2761 ccgctcacgg caagctagca tccgtgccgt aatcccgccc tttgacaatc gaggatcgcg
     2821 tgacgcatgt gagcgactcg caggccgcgc tggagc
//
LOCUS       DBNGZW010000376        14011 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1388724, whole genome shotgun sequence.
ACCESSION   DBNGZW010000376 DBNGZW010000000
VERSION     DBNGZW010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 14011)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14011)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14011
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1388724"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="AC16JC_08790"
     CDS             <1..213
                     /locus_tag="AC16JC_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164832.1"
                     /translation="ERARWLWLTAHRLSDLRRLVRQYDRAADAVFPTGPYFKSQQPTY
                     GTDVNFPIPLDEANNPNFAQCLDRNP"
     gene            complement(290..736)
                     /locus_tag="AC16JC_08795"
     CDS             complement(290..736)
                     /locus_tag="AC16JC_08795"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR5164833.1"
                     /translation="MNRHRPWLGLLAAPLSFLVACSHSAANRSRTGHTAGGRLITAEQ
                     IERSGSHTAWEVLKHEAPMLTLEEDRNGQPAHLQWRGRSSVYLDDAPIVVLDGVRVPD
                     WKVLVQIPAGEIEFINILTGIEGTTFYGTNAVSGVIEIRTKRGPDS"
     gene            867..940
                     /locus_tag="AC16JC_08800"
     tRNA            867..940
                     /locus_tag="AC16JC_08800"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:901..903,aa:Arg,seq:ccg)
     gene            1231..8244
                     /locus_tag="AC16JC_08805"
     CDS             1231..8244
                     /locus_tag="AC16JC_08805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164834.1"
                     /translation="MTCHSDNPVKTCTVGAVAFTLQPGTTAAGANLPVAVTVRDGSGN
                     TLTCFNDQVTIALGANPGNAILNGTATVTAASGVAQFSSLNLTKAANGYTLTASVGNT
                     TAASSPFNIIAGPASALAFTTPPSPTVAAGAVISVVVTARDQFGNTATGFVNSVTMAI
                     GNNPGSATLGGTTAVIASSGVASFPNLSIGKVGTGYTLTAAPTAGAPPAGATSVAFNV
                     NAGATAKLSFTVEPTNITAGSAISPAIQVTAQDAQGNPTPTFAGPVTIAIATNPGTGT
                     LAGTATVNASSGVATFPGLSINRPGIGYRLAATAPSLQSDTSSAFDVAIGPPAQLVFS
                     DEPTDAVAGVAIAPVTVTALDASGNPVTTFTGNVTVAIGTNPGSGTLTGTSTKAAVAG
                     VATFNDLSIDKVAGGYTLTATGAGASVSSSSFSVSAGPASVLEFTTQPANVAAGAALS
                     TTVIARDQFGNPATSFNGSVTISIGTNPGSGTLSGSTAVAASAGVAAFSGLSINKMGT
                     GYTLVATATAGAPPAPATSLAFNVSAGSVAKLAFTVSPVNGSAGSAITPAIEVTAQDA
                     EGNITPTFTGNVTLALGANPGGATLTGGGAVPAVGGVATFSGVSLNKPGTGYTLIATS
                     GALTPATSVPFNIAVGALAQLVFTQQPGNAVAGVSIAPAVVVTAQDAAGNTVTGFTGN
                     VTVAIGTNPGSGTLSGTPTKAAVAGVVTYNSLSINKSGAGYTLTATAGGVPQIASIAF
                     NIAPATAAKLSFSAPGAQQPSTTSAGAAITPPVKVTALDQFDNLDTSFTGNVSAAIGT
                     NPKSGTLTGTTTIAAAAGVATFSTLKIDSAAVGYTLTATASGLTDGTSSTFDVTPGTA
                     THLVFTQQPTNATAGAGIAPAVKVTALDAHGNVAAGYTANVTVAIGNNAGGGTLSGTA
                     TVAAAAGVATFTGLSIDKSGLGYTLTAASGALTGTASATFNIVAGAATHLTITTQPVT
                     TAAGAAIAPAVVVAAQDALGNTDLTYAGNVVAAIGTNPGSGVISGTLTRAASAGIATF
                     DDLNIDLVGTGYTLSFTSGALTAATSTAFDIFANTATHLVFSVQPANAEAGVAIAPAI
                     VVQALDGSNNVATGFTGGVTIAIGTNAGGGTLSGTTSVSAASGVATFSTLSIDKVGDG
                     YTLTATAGGVTAITSSGFKITPAAATHLAFFAPPTNDTAGKAVTPAVVVHALDGFNNI
                     DTTYTANVDVSIGINPAGGTLSGTTSKASVHGVATFSDLSINKSGAGYTLHATSGTLT
                     DATSGAFDISPAALSLLVFTQQPTAVLAGAAIQPDVVVQAQDAFANVVTSFTGSVGIA
                     IGTNGGGGALGGTTAVNAIAGAATFSNLTINKAGSGYTLVASTSGAPDATSTGFDVTA
                     GAASVLVFTVQPAPSTPAGTAISSPVQVAAQDGLGNTVASFANDITVALTGGTANAHL
                     SGTKTITPTNGVAVFSNLSIDSAGTGYTLSATAAGGGLSGGTSNAFAITPGTVTKLAF
                     TVGPVTTTAGQAIPTITVNAQDALGNTDPGYTSDVVVAITGGSGKTGAHLSGTLTHAA
                     LAGAASFPGLSIDSAGTGYTLTATSGSLTNAVSASFSINAGPVTQLLFTQQPSGAQAG
                     SAISPAVTLLAKDAIGNVNTGYVATVTLSLAVNPGGATPSNNAVGAVAGVAAFPGLFL
                     DKAGTGYTLQASDGSVISAPSNAFNITPGAASTLAFTVQPSTVTVGANISPAVKVAAQ
                     DAFGNLVTSFSNNVSMNLATNPGPGTLGGSLTRAAISGVATFSTLDINVAASGYRLGA
                     ATSGLAPATSAAFNVIPSAAVALFFKIQPSRTTAGQTIAAFTVEARDASGQLVSGYGD
                     DVTLSITPNTGVAGASLGGTVTQAASAGTATFNDIFINKAGDDFKLTATAAGVTSALS
                     GFFKIDPAAADHLVYTVQPSNVTALSPITPQIEVTAFDAFGNVSTYTGNVTMSIGTNP
                     ASGTLSGTVTKAAVAGVATFSNLSINNAGQGYTLDATASPALATSSVVGSSQFDVTAS
                     TANHLVFASQPTGVTADQTIPTFTIEARDAANSLLTGFTGDVTATITAGTGTSGATLS
                     GTTTVAAIGGVATFSGLAIDKKGTGYRLSATASGLAGTTSSTFAITAGTPTHVVFIQD
                     PTNAVAGTAVAPAILVDVEDAHENRVTSFVGTVTLAISTNPTGDTLRGTVTRTVVNGL
                     ATFDDIGIGLAAQGYRLQATSGGLTADVSQAFTITAGTATRLVFTVAPSNAAANAGIN
                     PAIRVAAFDGFDNIATGFNGSGNNVTLAITGGTGTAGATLSPTNPVVVSPVNGVATFS
                     GVSINLTGTGYTLTATATGVTQAVSAPFNIN"
     gene            8270..8431
                     /locus_tag="AC16JC_08810"
     CDS             8270..8431
                     /locus_tag="AC16JC_08810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164835.1"
                     /translation="MTNSPADSGRAVAFEWRSYSPPRARAAAVTRSIHATKYRASSLE
                     RTGAPPTVT"
     gene            complement(8474..9697)
                     /locus_tag="AC16JC_08815"
     CDS             complement(8474..9697)
                     /locus_tag="AC16JC_08815"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013939444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmpA family aminopeptidase"
                     /protein_id="HIR5164836.1"
                     /translation="MILLLAASLVVQQPAAPTKPRARDLGVPFDGTAGPLNAITDVPG
                     VEVGQVTLIAGSGPLVVGKGPVRTGVTAILPRGRAGGGDPVMAGWFTLNGNGEMTGTT
                     WLEETGFLRGPVMITNTYSVGVVRDAVVGWLVKQGLDWDWSLPIVAETWDGGLNDIEG
                     FHVRREDAFRALDSARGGPVAEGDVGGGTGMICHRFKGGIGTASRRVTIDDRIYTLGV
                     LVQCNYGTRPQLHIAGVPVGREIADQLPCYATAGPLPTGDERLSRCPVPGSRAPGPGG
                     GRGDVEQGSIIVVVATDAPLLPHQLKRVAKRVTLGVARMGGIGGDSSGDIFVAFSTAN
                     PGAAAASGRPTATWIPNEEINPVFEATAQATEEAIVNALVAAETMTGANDLRVTALPH
                     DRLRAVLQKYNRLAK"
     gene            complement(9702..10445)
                     /locus_tag="AC16JC_08820"
     CDS             complement(9702..10445)
                     /locus_tag="AC16JC_08820"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004970028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR5164837.1"
                     /translation="MMAAVAARELRDGEVVFVGIGLPNLACNLARATHAPNLVLIYES
                     GAVGAVPERLPVSIGDPALVTGSLMVCGMADVFQCFLQNGRIEVGFLGGAQVDRWGNI
                     NTTVIGEYTHPKVRLPGSGGATEIAAHARRTLVMAKLDPRAFPEQVDFLTSPGHRCHG
                     RSRDELGLPGAGPVKVITDKAVLEADPHTGELLLIALYPGVTPDEVRAGVGWPLATRT
                     TLLGVPPPNARDLRLLREVLDPKKLFLKG"
     gene            complement(10468..11382)
                     /locus_tag="AC16JC_08825"
     CDS             complement(10468..11382)
                     /locus_tag="AC16JC_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003965423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR5164838.1"
                     /translation="MTDHASKVVSMHDAIAAHVHDGDTVVIEGFTHLICFAAGHEIIR
                     QRRRNLTLARLTPDLIYDQMIAAGCAKKLVFSWAGNPGVGSLHAFRRAAEGKGPPLEL
                     EEYSHFGMVARFQAGAARLPFWTLRDYAGTDLPRVNAAIRSVTCPYTGETLATVPALN
                     PDVTIVHAQRADAAGNTQIWGLVGVQKEAAFASSRVIVVVEELVDTAVIRADPNRTLI
                     PGIAVSAVVVEPWGAHPSYAQGFYDRDNDFYVRWEGISRDPARLEAYLEEFVVGVPDR
                     AAYLARHPGLTGRLRARNQVCAGVNYGF"
     gene            complement(11435..12772)
                     /locus_tag="AC16JC_08830"
     CDS             complement(11435..12772)
                     /locus_tag="AC16JC_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HIR5164839.1"
                     /translation="MSGAARAYPRIIVPPPGPKARAVVAQHDRYASTSYPKEYPLVIA
                     GGDRAMVEDVDGNRFLDFMAGIAVTSTGYNHPAVVAAVQEQAGRFLHICGADFYFAGF
                     SRLCERLATLAPGPSPKRVFLSNSGAEAVEGAMKLARHSTGRAAFVAFSGSFHGRTYG
                     ALSLTASKAVQRSGFGPFLPEVYHVPYPYQYRCDFCRGEPQCTRGCLRNIDEDLFGRR
                     LDPQNVAAIVVEPIQGEGGYVVPPDGFLRDLRALCDRHGILLICDEVQSGIGRTGKMF
                     ACEHEGIEPDVITVAKGLGSGMPIGALIAKAAVMKWGPGAHGSTFGGNPVSCAAALVT
                     LDLVEREFMGNARVQGERLLAGVRRLADKHRTIGEVRGRGLMIGIEFVADRATRAPHP
                     DLVGKLERLAFSKGLLLLGAGKSSLRLAPPLVVDDYDVDTALSILDQCLEELE"
     gene            complement(12780..>14011)
                     /locus_tag="AC16JC_08835"
     CDS             complement(12780..>14011)
                     /locus_tag="AC16JC_08835"
                     /inference="COORDINATES: protein motif:HMM:NF012398.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5164840.1"
                     /translation="KYELAALMSLEVGKNRLESMGDAEESADLIDYYCTQVEDANGFV
                     REMAQVTPAERNMDVLRPYGVFACIAPFNFPLALSTGMSAAALVAGNAVVYKPSEDAP
                     WTGLRLTEVYRDAGLPPGTFNYLVGRREDIGDYLWQHEGVDGVVFTGSKTVGLRIHHG
                     ISTRWIKPALMELGGKNACLVLASADLDAAADGVLRSAFGLQNQKCSATSRVYVHRAV
                     AGPFVERLLEKTAAMTIGDPTQRDVYFGPVINERAVARYEASVAQANLEGKVLTGGRR
                     LTEGALAKGHYVAPTVARLPLESTLFREELFVPLLAVGEVGSFEEAIAETNKADYGLT
                     AGIFTAEPREIERFFDEVQAGVCYANRRSGATTGAWPGAQPFCGWKGSGSTGKGGCGP
                     YYVAQFMREQSRTVMGQ"
BASE COUNT         2296 a         4914 c         4795 g         2006 t
ORIGIN      
        1 gagcgcgctc gctggctgtg gctcacggcg caccgcttga gcgacctgcg acgcctggtg
       61 cggcagtacg accgggcggc cgacgcggtc ttcccgaccg gcccgtactt caagagccag
      121 cagccgacct acggaacgga cgtcaacttc ccgattccgc tggacgaggc gaacaacccg
      181 aacttcgcgc aatgcctcga ccgtaacccg taggcggaca ccagtccgcg cggacgaggg
      241 cagggggcgg acccgacccc ctgccctcgt tcccattccg gtctgacgct caggagtccg
      301 ggccgcgctt ggtgcggatc tcgatcaccc cgctgacggc gttggtgccg tagaaggtgg
      361 tcccctcgat cccggtcagg atgttgatga actcgatttc tccggccggg atctggacca
      421 ggactttcca gtccgggacc cgcacgccgt ccaggacgac gatgggggca tcgtcgaggt
      481 acacgctgga ccgcccccgc cactgcaggt gtgccggttg gccgttgcga tcctcctcca
      541 gcgtgagcat cggcgcttcg tgtttcagca cctcccaggc ggtgtggctg ccggaccgct
      601 cgatctgctc ggcggtgatc agccggccgc ccgcggtgtg tccggtgcgg gagcggttcg
      661 cggcgctatg ggagcaggct acgaggaacg acagcggggc ggcgaggagt ccgagccacg
      721 gacgatgccg gttcatgggg accccaggaa ggaacgcgac ggcgtgcgtg gattgccgaa
      781 tagtcccgcc cctgggggcg aaaggcaagt gcagattcgg gggaggctgc cactcctcgt
      841 gatgggtctg tatcttgagg gcccacgcgc cagtagctca gctggataga gcgtcggcct
      901 ccggagccga aggtcatggg ttcgaatccc gtctggcgca tccgcacgaa gcaaggcccc
      961 gggggccttg ggcacagaag ctgctacgac ccaccgtctc ggatgtggga cacttactga
     1021 caacctcatg ggaaattacg agttggacgg gctttcggaa ttagaatgaa cgccgatgaa
     1081 ggttacttta gtcccccccg ttccattcac caccgtgcat tagaggacac tcgcctgcgt
     1141 tctgccaggt cggttccccg tcacgcccct cgcccgctct ccgtccgtct agcgcgagcg
     1201 gggtccgcag ccgcccttgc cttgaaggtg gtgacgtgcc actcggacaa ccccgtcaag
     1261 acgtgcacag tgggtgcagt cgcgttcacg ctgcagcccg gcacgacagc cgcgggagcc
     1321 aacctcccgg tcgccgtgac ggtccgagac ggctcgggga acacgctgac ctgtttcaac
     1381 gaccaggtca ccatcgcgct gggcgcgaat ccgggcaacg cgatcctcaa cgggaccgcg
     1441 accgtgaccg ccgcgagcgg cgtcgcccag ttcagcagcc tcaacctcac caaagccgcg
     1501 aacggctaca ccctcacggc aagcgtcggg aacacgaccg cagccagcag ccccttcaac
     1561 atcatcgccg gtccggcgag cgcgctcgcg ttcaccaccc caccctcccc cacggtagcc
     1621 gcgggggcgg tgatctcggt ggtcgtgact gcccgcgacc agttcggcaa cacggccacg
     1681 ggcttcgtga acagtgtcac gatggcgatc gggaacaacc ccgggagcgc caccctgggc
     1741 ggcacgacgg ccgtcatcgc cagcagcggg gtcgcgagct tccccaatct ttccatcggc
     1801 aaggtcggca cggggtacac gttgacggcc gcgccgacgg ccggagcgcc gcccgcgggc
     1861 gcgacgagcg tggcgttcaa cgtcaacgcc ggggcgaccg cgaagctctc cttcacggtg
     1921 gagccgacga acatcacggc cggatcggcg atcagtcccg ccatccaggt gacggcgcag
     1981 gacgcacagg gcaacccgac acccaccttc gcgggcccgg tcacaatcgc gatcgcgacg
     2041 aatcccggga cggggacgct ggcgggcacc gccacggtca acgcgtcgag cggtgtcgcc
     2101 acattcccgg gattgagcat caacaggccg gggatcggct acaggctcgc ggccacggca
     2161 ccgtcgctcc agtccgacac gagcagtgca ttcgacgtgg ccatcgggcc gccggcccag
     2221 ttggtcttca gtgacgagcc gaccgatgcc gttgcgggag tcgcgatcgc gccggtgacc
     2281 gtcaccgcgc tggacgcctc cgggaacccc gtcacgactt tcacgggcaa cgtcacggtg
     2341 gcgatcggga caaatcccgg ttcgggtacg ctgacgggca cgtcgaccaa ggcggcagtc
     2401 gcaggcgtcg cgacattcaa cgatctgagc atcgacaagg tggccggtgg ctacacgctg
     2461 acggctaccg gtgccggagc gagcgtgtcg agctcttcgt tcagcgtctc cgccggtccg
     2521 gcgagtgtgc tcgagttcac gacccagccc gccaacgtgg ccgcgggcgc ggcactctcc
     2581 accacggtga ttgcgcgcga ccagttcggc aatccggcga cgagcttcaa cggcagcgtc
     2641 acgatctcga tcgggacgaa tcccggaagc ggaaccctgt cgggcagcac ggccgtggcc
     2701 gccagcgcgg gcgtggccgc gttcagcgga ctttccatca acaagatggg gacgggttac
     2761 accctggtcg ccaccgcgac cgccggcgcc ccgccggccc cggcgaccag cttggcgttc
     2821 aacgtcagtg ccggatcggt cgcgaagctg gcattcacgg tgtcgcccgt gaacggatcc
     2881 gccgggtcgg cgatcacgcc ggcgatcgaa gtgacggcgc aggacgccga ggggaacatc
     2941 acgcctacgt tcacgggcaa cgtgaccctc gcgctcgggg caaaccccgg cggcgcaacg
     3001 ctgacgggcg gcggagccgt gcccgccgtg gggggcgtcg ccacgttctc cggcgtcagc
     3061 ctcaacaagc cgggtaccgg ttacacgctg atcgccacct cgggggcgct cacgccggcc
     3121 accagtgtcc ccttcaacat cgcggtgggc gcgttggcgc agctcgtgtt cacgcagcag
     3181 ccgggcaacg ccgtcgccgg cgtatccatc gccccggccg tagtggtgac cgctcaggac
     3241 gccgccggta atacggtgac gggtttcacc ggcaacgtga cggtggcgat cggcacgaac
     3301 ccgggatcgg ggaccctgtc ggggacgccg accaaggcgg ccgtcgcggg agtggtgacc
     3361 tacaacagct tgagcatcaa caagagcggc gcgggttaca ccctgacggc caccgccggg
     3421 ggcgtgccgc agatcgcctc gatcgcgttc aacatcgccc cggcgacggc ggccaagctg
     3481 tcgttcagcg caccgggcgc ccagcagccg tcgaccactt cggcgggcgc ggcgatcacg
     3541 ccgcccgtca aagtcacggc gctggaccag ttcgacaatc tggacacgag cttcaccggc
     3601 aacgtgtcgg cggcgatcgg caccaatccg aagtcgggga cgctgaccgg cacgacgacg
     3661 atcgcggcgg cggcgggcgt agccacgttc tccacgctga agatcgacag cgccgcggtg
     3721 ggctacacgc tgaccgcgac ggcgagcggt ctcacggacg gcacgagctc gaccttcgac
     3781 gtcacgccgg ggacggccac gcatctggtg ttcacgcagc agcccacgaa cgctacggcg
     3841 ggagcgggga tcgcaccggc cgtgaaggtg acggcgctcg acgcccatgg gaacgtcgcg
     3901 gcggggtaca cggcgaacgt gaccgtggcg atcgggaaca atgcgggcgg cggcacgctg
     3961 tccggaacgg cgacggtggc cgcggccgcg ggcgtcgcca cattcacggg gttgagcatc
     4021 gacaagagcg gcctcggtta cacgctgacg gccgcctcgg gcgcgctgac cgggacggcc
     4081 agtgccacct tcaacatcgt cgccggcgcg gcgacgcacc tgaccatcac gacccagccg
     4141 gtgaccaccg ccgccggggc cgcgatcgct ccggcggtcg tcgtcgcggc gcaggacgcg
     4201 ctgggcaaca ccgacctcac gtatgccggc aacgtcgtgg cggcgatcgg gacgaatccg
     4261 gggagcggcg tgatctcggg caccctgacg cgggcagcga gcgccgggat cgccaccttc
     4321 gacgacctga acatcgatct cgtcgggacg ggctacacgc tgagcttcac gtcgggagca
     4381 ctcaccgccg cgacgagtac ggcattcgac atcttcgcca acacggcgac ccacctggtc
     4441 ttcagcgtcc aaccggccaa tgccgaggcc ggtgtagcga tcgcgccggc gatcgtggtg
     4501 caggcgctcg acggatcgaa taacgtcgcc accggcttca ccggcggcgt gacgatcgcc
     4561 atcgggacca atgcgggcgg cggcacgctg tcggggacga cgtcggtgtc ggcggcgtcc
     4621 ggcgtcgcga cgttctcgac gctcagcatc gacaaggtgg gtgacggcta taccctgacc
     4681 gccacggcgg gcggcgtgac ggccatcacg agcagcgggt tcaagatcac gccggcggcg
     4741 gcgacgcacc tggcgttctt tgccccaccg acgaacgaca ccgccgggaa ggccgtcacg
     4801 ccggcggtgg tggtgcacgc gctggacggc ttcaacaaca tcgacaccac gtatacggcg
     4861 aacgtcgatg tgtccatcgg gatcaacccg gccggcggaa cgctctcggg gacgacgtcc
     4921 aaggcctccg tacacggcgt ggcgacgttc tcggatctca gcatcaacaa gagcggcgca
     4981 ggctacacgc tgcacgcgac atcggggacg ttgaccgacg ccacgtcggg ggcgttcgat
     5041 atcagcccgg cggcgctgtc gctgctcgtc ttcacgcagc aacccacggc cgtcctcgcg
     5101 ggcgccgcga tccagccgga cgtcgtggtc caggcgcagg acgcgttcgc caacgtcgtg
     5161 acgtccttca cgggatccgt cggcatcgcg atcggcacga acgggggtgg tggagcgctc
     5221 ggcgggacga cggcggtcaa cgcgatcgcg ggcgccgcca cgttctcgaa cctcaccatc
     5281 aacaaggcgg gcagcggtta cacgctcgtc gcttcgacga gcggcgcgcc ggacgccacg
     5341 agcaccgggt tcgacgtgac cgcaggcgcc gcgagcgtgc tcgtcttcac ggtccagccg
     5401 gccccgagca cccccgcggg gactgcgatc tcctccccgg tccaggtggc ggcccaggac
     5461 ggcctgggca acacggtggc gagtttcgcc aatgacatca cggtggcgct cacgggcgga
     5521 acggccaacg cccacctctc ggggacgaag acgatcaccc ccacgaacgg ggtggccgtg
     5581 ttctcgaacc tgagcatcga cagtgccggg accgggtaca cgctctccgc cacggccgcg
     5641 ggggggggtc tcagcggcgg cacgagcaac gcgttcgcca tcacgccggg gaccgtgacc
     5701 aagctggcgt tcaccgtcgg tccggtgacg acgacggcgg gccaggcaat cccgacgatc
     5761 accgtcaatg cccaggatgc cctggggaac accgatcccg gctacaccag cgacgtcgtg
     5821 gtcgcgatca ccggcggcag cggcaagacg ggagcgcacc tctcgggcac cctgacccac
     5881 gcggcattgg ccggcgcggc gagcttcccg ggcctctcca tcgacagcgc gggcacgggg
     5941 tacacgctca ccgcgacgag cgggagtctg acgaacgccg tcagcgcgag cttcagtatc
     6001 aacgccggtc ccgtgaccca gctgctgttc acccagcaac cgtccggcgc gcaggcgggc
     6061 agcgcgatct cccccgccgt cacgctgctg gcgaaggacg cgatcggcaa cgtcaacacc
     6121 ggctatgtcg ccaccgtgac gctctcgctg gcggtgaatc ccggcggcgc cacgcccagc
     6181 aacaacgcgg ttggggcggt ggcgggcgtg gctgcgttcc ccggtctgtt cctcgacaag
     6241 gcgggaacgg gatacacgct gcaggcgtca gacgggagcg tgatttcggc gccgtccaac
     6301 gccttcaaca tcacgcccgg cgcggcgagc accctggcct tcaccgtcca gccctcgacc
     6361 gtcaccgtgg gtgcgaacat ctcgccggcg gtcaaggtgg ccgcccagga cgccttcggg
     6421 aacctcgtga cgagcttcag caacaacgtc agcatgaacc tcgcgacgaa ccccggtccg
     6481 gggacgctgg gcggcagcct cacgcgggcg gcgatctccg gcgtggcgac cttctcgacg
     6541 ctggacatca acgtcgcggc ctccgggtat cgactcggcg ctgcgaccag cgggctcgcg
     6601 ccggcgacga gcgccgcgtt caacgtcatc ccctcggcgg cggtggcgct cttcttcaag
     6661 atccagccgt cccgcaccac cgcgggccag acgatcgcgg cgttcacggt ggaagcgcgt
     6721 gacgcatcgg gccagctggt cagcggatac ggtgacgacg tcacgctctc catcacgccc
     6781 aacaccggcg tggcgggggc gagcctgggc ggaacggtga cgcaggcggc gagcgcaggc
     6841 acggcgacgt tcaacgacat cttcatcaac aaggccgggg acgacttcaa gttgaccgcc
     6901 acggccgcgg gcgtgacgtc cgcgctcagc gggttcttca agatcgaccc cgcggcggcg
     6961 gaccatctgg tgtacacggt gcaaccgtcc aacgtcacgg cgttgtcgcc gatcacaccg
     7021 cagatcgagg tcacggcctt cgacgcgttc gggaacgtct ccacgtatac cggaaacgtc
     7081 acgatgtcca tcggcacgaa tccggccagt ggtacgctga gcgggacggt gaccaaggcc
     7141 gcggtggccg gggtggcgac attcagcaac ctgagcatca acaatgccgg acagggctac
     7201 acgctcgacg ccaccgccag cccggcgctc gcaacgtcct cggtggtggg cagtagccag
     7261 ttcgacgtca cggcatccac ggcgaaccac ctggtcttcg cctcgcagcc aaccggcgtg
     7321 accgccgatc agacgatccc gacgttcacg atcgaagcgc gcgacgcggc gaacagtctg
     7381 ctgaccggct tcaccggcga tgtgacggcg accatcacgg ccggcacggg caccagcggc
     7441 gccacgctct ccggcaccac gaccgtcgcc gcgatcggcg gcgtcgccac cttctccggg
     7501 ctggccatcg acaagaaggg cacgggctat cggttgtcgg ccaccgcttc gggtctcgca
     7561 gggacgacga gcagcacctt tgccattacg gccggcacgc cgacgcatgt ggtgttcatc
     7621 caggatccga cgaacgcggt cgcgggaacg gcggttgcgc cggcgatcct ggtggatgtc
     7681 gaggacgccc acgagaaccg cgtgaccagc ttcgtcggca cggtgacgct cgccatctcg
     7741 acgaacccca cgggagatac gctccggggg acggtcacgc gcacggtcgt gaacgggctc
     7801 gcgaccttcg acgacatcgg catcggcctg gccgcgcagg ggtaccgtct ccaggcgacg
     7861 tcgggagggc tgaccgccga tgtgagccag gcgttcacca tcaccgccgg cacggcgacc
     7921 cgcttggtct ttacggtagc accgagtaac gcagccgcca acgccgggat caacccggcg
     7981 atccgggtcg cggcgttcga cgggttcgac aacatcgcga ctggcttcaa cgggagcggg
     8041 aacaatgtga cgctcgcaat taccggtggc accgggacgg caggcgccac gctgagcccc
     8101 accaaccctg tcgtcgtatc ccccgtgaat ggggttgcga ccttcagcgg cgtcagcatc
     8161 aatctcacgg ggacaggcta caccctgacc gccaccgcaa cgggggtcac ccaggcggtc
     8221 agtgcgccct tcaacatcaa ctagtatcgg gacacgctcc gagggaggga tgacgaacag
     8281 cccggccgat tccggccggg ctgttgcgtt cgagtggagg tcctactccc cgccgcgcgc
     8341 ccgtgccgca gccgtgaccc gatcgatcca cgccacgaag taccgcgcgt cctcgctcga
     8401 gcgcaccggt gcgccgccga cggtcacgta gaccggggat gtcgaggcaa ggggggtgcg
     8461 acggggcgca tgctcatttc gccaggcgat tgtacttctg caacaccgcc cgcaaccggt
     8521 cgtgcggcaa cgccgtcacc cgcaggtcgt tcgccccggt catcgtctcg gcggcgacca
     8581 gggcattgac gatcgcctcc tcggtggcct gcgctgtcgc ctcgaacacc ggattgatct
     8641 cctcgttcgg gatccaggtc gcggtcggac ggccgctcgc cgccgcggcg cccggattgg
     8701 cggtcgagaa ggcgacgaat atgtcgcccg agctgtcgcc gccgatgccg cccatgcgcg
     8761 cgacgccaag ggtgacgcgc ttggcgacgc gcttcaattg gtgcggcagc agcggcgcgt
     8821 cggtggcgac gacgacgatg atcgacccct gctccacatc cccgcgccca ccccccggac
     8881 ccggggcccg ggacccgggg accgggcacc gtgagagccg ttcgtcccct gtgggcaacg
     8941 gtcccgccgt cgcgtagcac ggcagctgat cggcgatctc gcgccccacc ggcactccgg
     9001 cgatgtgcag ctgcggccgc gtgccatagt tgcactgcac caggaccccg agggtgtaga
     9061 tccggtcgtc gatcgtcacg cggcgcgacg ccgttccgat cccgcccttg aagcggtggc
     9121 agatcatccc ggtgccgccg cccacgtccc cttccgccac gggtccgccg cgggcgctgt
     9181 ccagagcgcg gaacgcatcc tctctcctga cgtggaaacc ctcgatgtcg ttcagcccgc
     9241 cgtcccaggt ctcggcgacg atcggcagcg accaatccca atccaatccc tgcttcacca
     9301 gccacccgac caccgcgtcg cgcacgacgc ccaccgagta ggtgttggtg atcatcacgg
     9361 ggccacgcag aaagccggtc tcctcgagcc aggtcgtgcc ggtcatctcg ccgttgccgt
     9421 tcaacgtgaa ccacccggcc atcacggggt cgccgccccc ggcccggccg cgcggcagga
     9481 tcgcggtcac gccggtgcgc acgggaccct tcccgaccac cagcggtccg ctgccggcaa
     9541 tcagggtgac ctgccccacc tccaccccgg gaacatcggt gatggcgttc aacggaccgg
     9601 ccgtgccgtc gaagggcaca cccaggtcgc gggcgcgcgg tttcgtggga gcagccggct
     9661 gctgcacgac gagcgacgcg gcgagcagga ggatcatgcg gtcatccctt gaggaacagt
     9721 ttcttggggt cgagcacctc gcgcagcagc cgcagatcgc gcgcgttggg tggggggacg
     9781 cccaacaggg tcgtccgtgt cgcgagcggc caaccgacgc ccgcgcgtac ctcatcgggc
     9841 gtcacgccgg ggtagagcgc gatgagcagc agctctcccg tgtgcgggtc ggcctcgagc
     9901 acggccttgt cggtgatcac cttcacgggc cctgcgccgg gaagaccgag ctcgtcgcgc
     9961 gaccggccat ggcagcgatg gccggggctc gtgagaaagt ccacctgctc ggggaacgcg
    10021 cgtggatcga gcttcgccat gacgagcgtg cgccgcgcgt gggcggcgat ctccgtggcg
    10081 ccacccgagc ccgggagccg cactttcgga tgcgtgtact cgccgatgac ggtggtgttg
    10141 atgttgcccc agcggtccac ctgcgcgccc cccaggaatc cgacctcgat gcggccgttc
    10201 tgcaggaagc actggaacac gtcggccatg ccgcacacca tgagcgaccc ggtgacgagc
    10261 gccggatcac cgatcgacac gggcaggcgc tcgggcaccg cgcccaccgc gcccgactcg
    10321 tagatcagca ccaggttggg cgcgtgggtg gcgcgggcga ggttgcaggc gaggttgggg
    10381 aggccgatac cgacgaagac gacctcgccg tcgcgcagct cgcgggcggc gacggccgcc
    10441 atcatctcgt cggccgtgta gtcccgctca gaagccgtag ttcactcccg cgcacacctg
    10501 attgcgcgcg cgcagccgcc cggtgaggcc cgggtgccgg gcgagatagg ctgcgcggtc
    10561 ggggacgccg acgacgaact cctcgagata ggcctcgagc cttgccgggt cgcgcgagat
    10621 cccctcccag cgtacgtaga agtcgttgtc ccgatcgtag aagccctggg cgtacgacgg
    10681 atgcgcaccc cacggctcga ccacgacagc cgagacggcg atcccgggaa tgagcgtgcg
    10741 gttgggatcg gcgcggatca ccgccgtatc gaccagctcc tccaccacca cgatgacgcg
    10801 gctcgaggcg aacgccgctt ctttctgtac gccgaccagc ccccagatct gggtgttgcc
    10861 tgccgcgtcg gcgcgctggg cgtggacgat ggtgacgtcc gggttcagcg ccggcaccgt
    10921 cgcgagcgtc tcccccgtgt aggggcaggt gacggagcgg atcgccgcgt tcacgcgggg
    10981 cagatcggtg ccggcgtagt cgcgcagcgt ccagaacggc agccgggccg ccccggcctg
    11041 aaagcgggcg accatgccga aatgcgagta ttcctcgagc tcgagcggcg ggcccttccc
    11101 ttcggcggcg cgccgaaagg cgtgcagcga gcccacgccg gggttgccgg cccaggagaa
    11161 gacgagcttt ttcgcgcacc ccgcggcaat catctggtcg tagatgagat cgggcgtgag
    11221 ccgggcgagc gtgaggttcc ggcggcgctg gcggatgatc tcgtgtcccg cggcgaagca
    11281 gatcaggtgg gtgaagcctt cgatgacgac ggtgtcgccg tcgtggacgt gggcggcgat
    11341 ggcgtcgtgc atggaaacga ctttggaggc gtggtcggtc atcggtcgtc ggtgctctgc
    11401 aaatccataa ccgatgaccg acaaccgacg accatcattc caactcttcc aggcactggt
    11461 cgagaatgga gagcgccgta tcaacgtcgt agtcgtccac gacgagcgga ggcgccaaac
    11521 gcagcgagct cttgcccgcg ccgagcagca gcagcccctt ggagaaggcg agtcgctcga
    11581 gcttcccgac gagatcggga tggggcgcgc gtgtggcgcg gtcagccacg aactcgatcc
    11641 cgatcatgag gccccggccg cgcacttcgc cgatcgtccg atgcttgtcc gcgaggcgtc
    11701 gcacgccggc caggaggcgc tccccctgga cgcgggcgtt gcccatgaac tcgcgctcga
    11761 ccaggtcgag ggtaacgagc gcggcggcgc aggagacggg attgccgccg aaggtcgagc
    11821 cgtgggcgcc cggcccccac ttcatcaccg cggccttggc gatcaacgcg ccgatcggca
    11881 tcccggagcc cagccccttg gcgacggtga tcacgtcggg ctcgatgccc tcgtgctcgc
    11941 aggcgaacat cttcccggtg cgcccgatgc ccgattgcac ctcgtcgcag atcagcagga
    12001 tgccgtgccg gtcgcacagc gcgcgcaggt cgcgcaggaa gccgtccggc ggcacgacgt
    12061 agcccccctc gccctggatc ggctccacca cgatggcggc gacgttctgc ggatcgagcc
    12121 ggcgcccgaa caggtcctcg tcgatgttgc ggaggcagcc gcgggtgcac tgcggctcgc
    12181 cgcggcagaa gtcgcagcgg tactggtagg ggtaggggac gtgatagacc tcgggcagga
    12241 acggcccgaa cccggagcgc tgcaccgcct tgctggcggt gagcgagagg gcgccgtagg
    12301 tgcggccgtg gaacgacccc gagaacgcga cgaacgccgc acggccggtc gagtgccgcg
    12361 cgagcttcat cgcgccttcg accgcctcgg cgccggagtt cgaaaggaac acccgtttcg
    12421 gactcgggcc cggagcgagg gtggcgagcc gctcgcacag ccgggagaat ccggcgaagt
    12481 agaaatcggc gccgcagata tggaggaacc ggcccgcctg ctcctgcacc gccgcgacca
    12541 cggccggatg gttgtagccc gtggacgtca cggcgatccc cgccatgaag tcgaggaagc
    12601 ggttgccgtc cacgtcctcg accatggccc ggtcgccgcc cgcgatgacg agcggatact
    12661 ccttggggta ggaggtcgac gcataccggt cgtgttgggc gacgacggcg cgcgccttgg
    12721 gaccgggggg cggcacgatg atgcgcggat aggcacgggc ggcgccgctc aagtgcccct
    12781 cattgaccca tcaccgtgcg gctttgctcg cgcatgaact gggcgacgta gtaggggccg
    12841 catccgccct tccccgtcga gcccgatccc ttccagccgc agaacggctg ggcgccgggc
    12901 caggcgccgg tggtggcgcc gctgcgcctg ttcgcgtaac acacccccgc ctgcacctcg
    12961 tcgaagaacc gctcgatctc gcgcggctcc gccgtgaaga tccccgcggt gagcccatag
    13021 tcggctttgt tcgtctcggc gatcgcctcc tcgaaactcc ccacctcgcc cacggcgagg
    13081 agcgggacga acagctcctc gcggaagagc gtgctctcga gcggcagccg ggcgacggtt
    13141 ggggcgacgt agtggccctt ggccagcgcg ccttccgtca ggcgccggcc gcccgtgagc
    13201 accttgccct cgaggttcgc ctgggcgacc gaggcttcgt agcgcgccac ggcgcgctcg
    13261 ttgatgaccg gtccgaaata cacgtcgcgc tgcgtcggat cgccgatggt catggcggcc
    13321 gtcttctcga gcagccgctc gacgaacggg cccgccacgg cgcggtgcac gtacacccgg
    13381 gacgtggcgc tgcatttctg gttctgcagg ccgaacgccg accgcaacac cccgtccgcc
    13441 gcggcgtcga gatccgccga tgccaggacg aggcaggcgt tcttgccgcc cagctccatc
    13501 aacgcgggtt tgatccagcg cgtcgagatg ccgtgatgga tccgcagccc cacggtcttg
    13561 gagccggtga agacgacgcc atccacgcct tcgtgctgcc agaggtagtc gccgatgtcc
    13621 tcgcgccgcc ccacgaggta gttgaacgtg ccgggcggga gtccggcgtc gcggtacacc
    13681 tccgtgagcc ggagcccggt ccagggagcg tcctcggaag gcttgtagac cacggcgttg
    13741 ccggcgacga gcgcggcggc ggacatgccg gtcgagaggg cgagggggaa attgaacggc
    13801 gcgatgcagg cgaacacgcc gtagggacgc agcacgtcca tgttgcgttc ggccggcgtc
    13861 acctgcgcca tctcgcggac gaacccgttg gcgtcctcca cctgggtgca gtagtagtcg
    13921 atcaggtccg ccgactcctc cgcatcgccc atcgattcga gccggttctt cccgacctcg
    13981 aggctcatca gggccgcgag ctcgtacttg c
//
LOCUS       DBNGZW010000377         6485 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_646798, whole genome shotgun sequence.
ACCESSION   DBNGZW010000377 DBNGZW010000000
VERSION     DBNGZW010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6485)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6485)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6485
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_646798"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(228..1013)
                     /locus_tag="AC16JC_08840"
     CDS             complement(228..1013)
                     /locus_tag="AC16JC_08840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5164841.1"
                     /translation="MRRFHLLEIEDQPWCPEFLRTAITDFLRALMEWFAPYAVAAPLL
                     SRALVQTGDTEILDLCAGGGGPWRDLLGRIPSAGGPAVRVRLCDLYPNHAAYARLGRL
                     SGGRITGEPDPIDATAVPARLGGFRTLFTALHHFPPDAARAILADAVRQGRGIAVFEI
                     TRRSPLGLIGMLFLPILALLLTPFIRPFRWDRLFWTYLLPVVPLVVWFEGTVSCLRTY
                     TPAELRQLVAGFDEYQWEVGTVRGPPLITLVTYLVGTPHPAQD"
     gene            complement(1020..1766)
                     /locus_tag="AC16JC_08845"
     CDS             complement(1020..1766)
                     /locus_tag="AC16JC_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164842.1"
                     /translation="MLTGRGRRSCSVRRALLVCALALACGDSTAPVRAPRTFHMGFSA
                     IPPRPDQAVTLQSLNLWTSSHADAAIMHISVPYLALLSGLTAPQEVRGNELGLANYYR
                     AKGLRLVITVDVTNGLDRAREDPVLDSLGRSITDSSVRRLYREYVLALDSILHPDYLG
                     LAAETNLIRLAAPDSVYQAVVTMTNAVAASIRSLGSAPQLFVSAQVETAWGRFQGGGY
                     AGIAQDLADFPPGTRPSRVRLLHKGALVVL"
     gene            complement(1760..2527)
                     /locus_tag="AC16JC_08850"
     CDS             complement(1760..2527)
                     /locus_tag="AC16JC_08850"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011683499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016403 - dimethylargininase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylarginine dimethylaminohydrolase family
                     protein"
                     /protein_id="HIR5164843.1"
                     /translation="MTLVALTRAVSPAIDRCQLVHLERRPIDLDLAREQHRGYEAALR
                     EAECTVRTLPADPSLPDSVFLEDTAVVLDEVAVIARPGAESRQPETEPVARVLAGYRP
                     LARITAPAKLDGGDVLSIGRTLYVGEGGRTNGAGIDQLGAAVAPHGYDVRSVAVHGCL
                     HLKSAVTLVAPDMVLCNPSWVDPSAFAGMRVLEVAAGEPYGANGLLIGVKLIYPSSFP
                     RTARRLVDQGIEILPLNVSELQKAEGAVTCCAILIAC"
     gene            complement(2524..4179)
                     /locus_tag="AC16JC_08855"
     CDS             complement(2524..4179)
                     /locus_tag="AC16JC_08855"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family metallopeptidase"
                     /protein_id="HIR5164844.1"
                     /translation="MVALPRAARLAAMLFWALPLGAQTRTSPDITPQDLRQRLYLIAD
                     DSMGGRRSGSAGDFQTAAYVASEFERLGLRPAGDSGTWFQTVPFFFAEPAASSLSAGE
                     ASLTAGTDYVLIGRPVATSLLTGIPAVYGGSATDSSGWIGADQASGRLVVFDIRAGGD
                     GQRRLAPLRGLATSTRFTHAAALAVAELDLLAPEIVAQIGERRLTADTARASGQPALL
                     LITPDAAARLLGAAPGRLAPGAVGARARGDLCVRFTPVPFPARNVVAVLPGADPALRG
                     QYVALTAHNDHVGFDHSPVDHDSLRALNRVIRPMGADSPNRPATAEERMQAGRLLDSL
                     RSRRPARRDSIRNGADDDGSGTVAILEIAEALATGPQRPRRSILFVSHTGEEEGLIGS
                     GWFTDHATVPTDSIVAEIDEDMVGRGTAADLPEGGPTYLEVVGARRLSREFGDLLEAA
                     NAAQPEPFRFNYAYDAPGHPLQYYCRADHYSYARYGIPSVALSRGEHLDYHQVTDEAQ
                     YIDYDDLARVARLVRDAALRVANLDHRPALDHPKGDPHAPCVQ"
     gene            complement(4245..5297)
                     /locus_tag="AC16JC_08860"
     CDS             complement(4245..5297)
                     /locus_tag="AC16JC_08860"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-aminocyclopropane-1-carboxylate
                     deaminase/D-cysteine desulfhydrase"
                     /protein_id="HIR5164845.1"
                     /translation="MSGTLFARYPGLRSRLPWLPLATLPTPVEEWTPPTDYRGRLFVK
                     RDDLTSPLYGGNKVRKFEHLFAAARRRDAHALVTLGGFGTNQGLALALHGRAAGFAVE
                     VALAPQPVTPEVRANLRGLLAAGAALRCARTGPGCVLEAWRAMRVRRAAGERPFFVPA
                     GASTALSTIGYVAAALELADQIEQGALPVPDRIFVAAGTCGTAAGLVAGCRLAGLPTR
                     VSAVRVFAAYRANRRTIGRLARDAMRLLRAWEPAIPDISISNRDFDVLTKFLGPGYGA
                     PTPQARAAIAWAAPRVPLETTYTGKALAACLATGNAAAGAPTVVFWNTYNSAAFPQTT
                     DPLLAPAPLRKVLTGP"
     gene            complement(5294..6271)
                     /locus_tag="AC16JC_08865"
     CDS             complement(5294..6271)
                     /locus_tag="AC16JC_08865"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HIR5164846.1"
                     /translation="MNDRWDARLAPFRRPSTARSVWQLVSTALLLAGAWTLMYFSLGV
                     GYWLTLLLAVPAAFFLVRLFIVQHDCGHGSFFRSPRVADFVGSALGVLTLTPYHYWKK
                     AHAMHHATSGNLEHRGFGDIDTLTVDEYQALSRWGRFKYRMYRHPVILFGVGAVLHFF
                     VRHRLPTIVPRTWTRARWSIFWTDVGLAAFVVGMGLLVGFRQFLLVHVPLMTLSCSMG
                     VWLFYVQHQFEPTYWEHDEQWAYNDAALVGSSYYRLPRLLQWATGNIGLHHVHHLSAR
                     IPNYRLQEALESIPELRRVTTLTLWESLRCVRLALWDERERKLVRFPRR"
     gene            complement(6268..>6485)
                     /locus_tag="AC16JC_08870"
     CDS             complement(6268..>6485)
                     /locus_tag="AC16JC_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164847.1"
                     /translation="HLPAQVNRSQFALSRKGLVLGSGPLECPGCGGRLEFGADRLGRT
                     TESCGCGYYALVRKRAGETGDEPRRSR"
BASE COUNT         1042 a         2305 c         2297 g          841 t
ORIGIN      
        1 gcgctgtcca ccaccacccc gctgactgcg gcctgctgcg caaccgcctg acgtccggcc
       61 gcttcccaca gccccgctgc gaccaccgcc caccaccacc acgaccgcgc gcccatgtgt
      121 caccctcgtg gttgaagttc accgcctcac ggcacgaccg agtacaggac cgccaatgcc
      181 ggcactgttg tggaaggaaa tgggacgatg aaagagccat gtccccgtca atcctgcgca
      241 ggatggggag tcccgacaag ataggtcacg agcgtgatga gcggcggacc ccggacggtc
      301 ccgacctccc attgatattc atcgaacccc gccacgagct gccgcagctc ggcgggcgta
      361 taggtgcgca aacacgacac ggtcccctcg aaccacacca ccagcggcac caccggcagg
      421 aggtaggtcc agaacagccg gtcccagcgg aacggccgga tgaagggcgt cagcagcagg
      481 gcgaggatgg ggaggaacag cataccgatg agtcccagcg gcgagcggcg ggtgatctcg
      541 aagacggcga tgccccgccc ctgacggacc gcgtccgcga gaatcgcgcg tgcggcgtcg
      601 ggcgggaaat gatgcagcgc ggtgaacaag gtacgaaacc cgccgaggcg cgcgggcacg
      661 gcggtggcat cgatcgggtc cggctccccc gtgatgcggc cgccgctcag ccggccgagc
      721 cgtgcgtagg ccgcgtggtt cgggtacaga tcgcacaggc gcacgcggac cgccggaccg
      781 cccgccgaag ggatccgccc gagcaggtcg cgccacggcc cgccgccccc agcgcagagg
      841 tccaggatct ccgtgtcgcc ggtctgcacg agggcgcgcg acaggagcgg cgccgccacg
      901 gcatacggcg cgaaccactc catcagcgcg cgcaggaaat cggtgatcgc ggtccggagg
      961 aactccgggc accacggctg gtcttcgatc tcgagcaggt gaaagcggcg cattggtggt
     1021 cagagcacga caagagcgcc cttatggagc aaacggacgc gcgaaggccg cgtcccaggc
     1081 ggaaagtcgg cgagatcctg ggcgatcccg gcgtaccccc cgccctggaa gcggccccac
     1141 gccgtctcga cctgcgcgct cacgaacagc tgcggggccg atcccaggga ccggatgctc
     1201 gccgccacgg cgttggtcat cgtcacgacg gcctggtaga ccgaatccgg cgccgccagc
     1261 cggatgagat tcgtctccgc cgcgagcccc aggtagtccg gatgcaggat cgagtcgagc
     1321 gccagcacgt actcccggta gagccgccgg acggacgaat cggtgatgct ccggcccagc
     1381 gaatcgagca ccgggtcctc acgggcgcgg tcgaggccgt tggtgacgtc caccgtgatg
     1441 acgagccgca gacccttcgc ccgatagtag ttcgccagtc ccagctcgtt gccccggacc
     1501 tcctgcggcg cagtcaggcc cgacaacagc gcgaggtacg gcaccgagat gtgcatgatc
     1561 gccgcatcgg cgtggctgga agtccagaga ttgagggatt gcagcgtgac ggcctgatcg
     1621 ggacgcgggg ggatcgcgga gaaccccatg tggaaggtgc ggggcgcccg gaccggtgcg
     1681 gtcgagtcgc cgcaggcgag ggccagtgcg cacacgagca acgcccggcg cacgctgcag
     1741 gaccgccgcc ctctcccggt cagcacgcga tcaagatggc acagcaggtg accgcccctt
     1801 ccgccttctg cagctcggac acgttcagcg gcaggatctc gatcccctga tccacgagcc
     1861 ggcgggcggt gcgcgggaag ctcgacgggt agatgagctt cacgccgatg aggagcccgt
     1921 tcgcgccgta gggctccccg gccgcgacct cgagcacccg catccccgcg aaggccgagg
     1981 ggtccaccca gctcggattg cacaacacca tgtccggcgc caccagcgtc acggcggact
     2041 tgagatgcag gcacccgtgc accgcgacgg agcgcacgtc gtatccgtgc ggcgccaccg
     2101 cggccccgag ctggtcgatt cccgcgccgt tggtccgtcc gccctccccc acgtacagcg
     2161 tgcgtccgat ggacagcacg tcgccgccgt ccagcttcgc cggtgcggtg atccgggcca
     2221 gtggccggta gcccgcgagc acccgggcga cgggttcggt ttcgggctgc cgcgattcgg
     2281 caccgggccg cgcgatgacg gcgacctcgt cgagcacgac ggcggtgtcc tccaggaaca
     2341 ccgaatccgg caacgacgga tcggccggaa gggtccgcac cgtacattct gcctcgcgca
     2401 gcgccgcttc gtacccacgg tgctgctccc gtgccaggtc caggtcgatg ggccgccgct
     2461 cgagatgcac cagctggcag cgatcgatcg cgggactcac cgcgcgggtg agcgcgacga
     2521 gggtcactgc acgcacggcg cgtgggggtc gcccttgggg tgatcgagcg cggggcgatg
     2581 atccaggttg gcgacccgca gcgccgcgtc gcgcaccagc cgcgccacgc gggccaggtc
     2641 gtcgtagtcg atgtactgtg cctcgtccgt cacctggtgg tagtccaggt gctcgccgcg
     2701 cgacagggcg acgctcggaa tgccgtagcg ggcatagctg taatgatcgg cgcggcagta
     2761 gtactgcagc ggatgtcccg gcgcgtcgta cgcgtagttg aacctgaagg gttccggctg
     2821 ggccgcgttg gccgcttcca gcaggtcgcc gaactcgcgc gacaggcggc gggcgcccac
     2881 cacctcgagg taggtcggtc cgccctcggg caggtcggcc gccgtgccgc ggcccaccat
     2941 atcctcgtcg atttccgcca cgatcgaatc ggtcggcacg gtcgcgtggt cggtgaacca
     3001 gcccgacccg atcagaccct cttcctctcc cgtgtgcgag acgaacagga tcgaccgccg
     3061 cgggcgctgc ggtcccgtgg cgagcgcctc ggcgatctcc aggatcgcca ccgtccccga
     3121 gccgtcgtcg tcggctccgt tccgaatcga gtcccgccgc gccggccgcc ggctccgcag
     3181 gctgtccagc aaccggcccg cctgcatccg ctcctcggcg gtcgcgggcc ggttgggcga
     3241 atcggctccc atggggcgga tcacccggtt gagggcgcgc agcgagtcgt ggtccacggg
     3301 gctatggtcg aagcccacgt ggtcgttgtg tgccgtcagg gcgacgtatt gcccgcgcag
     3361 cgccggatcg gctccgggga gcaccgccac cacgttgcgc gcgggaaacg ggaccggggt
     3421 gaagcgcacg cagaggtcgc cccgcgctcg agcccccacg gccccgggcg cgagtcgccc
     3481 gggagcggcc ccgagcaggc gagcggccgc gtccggcgtg atcaggagca gcgcgggctg
     3541 tccgctcgcg cgcgcggtgt cggcggtcaa acgccgctcc ccgatctgcg cgacgatttc
     3601 gggggcgagg agatcgagct ccgcgacggc gagcgccgcg gcgtgcgtga agcgcgtact
     3661 ggtcgcgaga ccgcgcagcg gtgccagacg ccgttgcccg tcgccccccg cacggatgtc
     3721 gaacaccacg agcctgccgc tcgcctggtc cgctccgatc cagcccgagg aatcggtcgc
     3781 cgagccgccg tagaccgccg ggatccccgt gagcagcgac gtcgcgaccg gccggccgat
     3841 cagcacgtaa tccgtgccgg ccgtgagcga ggcctccccg gccgacagcg acgacgcggc
     3901 cggctcggcg aagaagaacg gcacggtctg gaaccaggtc ccactgtccc ccgccggtcg
     3961 caagcccagc cgctcgaact cggacgccac ataggcggcc gtctggaagt ctcccgccga
     4021 ccccgagcgc cgtccgccca tcgaatcgtc ggcaatgagg tagagccgct gccgcaggtc
     4081 ctgcggcgtg atgtcgggtg atgtcctcgt ctgggctcca agcggcagcg cccagaacag
     4141 catggcggcg agtcgagcgg cgcgaggcaa tgcgaccacg agggtagtct ccgttcgagg
     4201 ggcgaggggc gaggcaaaag tacgcgccgg cggcgcgccc tgcgtcaggg gccggtgagc
     4261 accttccgga gcggggccgg ggccagcaga ggatccgtgg tctgcggaaa ggcggccgaa
     4321 ttgtaggtgt tccagaagac cacggtaggc gcgccggcgg ccgcgttgcc ggtggccagg
     4381 caggccgcca gcgccttccc cgtgtaggtc gtctccagcg gcacccgggg agccgcccag
     4441 gcgatggccg cgcgcgcctg cggcgtcggg gcgccgtacc ccggaccgag aaacttcgtc
     4501 agcacgtcga aatcccggtt gctgatggag atgtccggga tggccggctc ccacgcccgc
     4561 agcagccgca tcgcgtcccg cgccagccgg ccgatcgtcc gccggttcgc ccggtaggcg
     4621 gcgaagacgc gcaccgcgct cacgcgggtc ggcagcccgg cgagccgaca tcccgccacg
     4681 agtcccgccg cggtcccgca cgtcccggcc gccacgaaga tccggtccgg gaccggcagc
     4741 gctccctgct cgatctggtc agccagctcg agggccgccg cgacgtagcc gatcgtggag
     4801 agcgcagtgg acgccccggc gggcacgaag aacggccgct ccccggccgc ccgccgcacc
     4861 cgcatcgccc gccacgcctc gagcacgcag cccggtcccg tgcgcgcgca gcgcagcgcg
     4921 gcgccggcgg cgaggagccc gcgcaggttg gcccggacct ccggggtgac cggctgcggc
     4981 gcgagcgcca cttccaccgc gaagcccgcc gccctgccgt gcagggcaag ggccagcccc
     5041 tgattggtgc cgaagccgcc gagcgtcacg agcgcgtgcg cgtcccgccg gcgtgcggcc
     5101 gcgaacaggt gttcgaactt gcggaccttg ttgccgccgt agagggggga ggtgaggtcg
     5161 tcgcgtttga cgaacagccg cccgcggtag tccgtgggcg gcgtccattc ttcgaccggt
     5221 gtggggaggg tggcgagcgg gagccagggc agccggctcc gcaggccggg gtagcgcgcg
     5281 aagagggtgc cgctcaccgc cgcgggaatc gcaccagctt ccgttcgcgc tcgtcccaga
     5341 gggcgagccg cacgcaccgc aggctctccc acagcgtcag cgtcgtgacc cgccgcagct
     5401 cgggaatcga ctccagcgct tcctgcaggc ggtagttggg aatgcgggca ctgagatggt
     5461 gcacgtggtg cagcccgatg ttacccgtcg cccattgcag cagccgcggc aggcggtaat
     5521 aggagctgcc gaccagtgcc gcgtcgttgt aggcccactg ctcgtcgtgt tcccaatagg
     5581 tgggctcgaa ctgatgctgg acgtagaaca gccagacgcc catcgagcac gagagcgtca
     5641 tgagcggcac gtgaaccagc agaaattggc ggaaccccac cagcagaccc atgccgacga
     5701 cgaacgcggc cagcccgacg tcggtccaga agatgctcca gcgcgcgcgt gtccaggtgc
     5761 gcgggacgat cgtcgggagc cggtggcgta cgaagaagtg caggacggcc ccgacgccga
     5821 agagaatcac cgggtggcgg tacatgcggt acttgaagcg cccccagcgc gacagcgcct
     5881 ggtactcgtc caccgtgagc gtatcgatgt cgccgaaccc gcggtgttcg agattgcccg
     5941 acgtggcgtg gtgcatcgcg tgggccttct tccagtaatg gtacggcgtg agggtcagca
     6001 ccccgagcgc cgagcccacg aagtcggcaa cgcgaggaga gcggaagaac gacccgtggc
     6061 cgcaatcgtg ctgcacgatg aacagccgca cgaggaagaa cgccgcgggc acggcgagga
     6121 gcagcgtcag ccagtatccg acgcccaggc tgaagtacat gagcgtccac gccccggcca
     6181 gcagcagtgc ggtgctcacg agctgccaca cgctgcgcgc cgtgctgggg cgccggaatg
     6241 gcgcgagtcg cgcgtcccac cggtcgttca tcgtgacctc ctcggctcgt cgccggtctc
     6301 gccggcccgt ttgcggacga gcgcgtaata cccgcagccg cacgattccg tcgtgcggcc
     6361 gagccggtcg gcaccgaact cgagccggcc gccgcacccg gggcactcca gtggcccact
     6421 gcccaacacc agtcccttgc gggacagtgc gaactgggac cggttcactt gtgccggaag
     6481 gtgat
//
LOCUS       DBNGZW010000378         5856 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1466806, whole genome shotgun sequence.
ACCESSION   DBNGZW010000378 DBNGZW010000000
VERSION     DBNGZW010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1466806"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /locus_tag="AC16JC_08875"
     CDS             <1..682
                     /locus_tag="AC16JC_08875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HIR5164848.1"
                     /translation="NWGTSYLVHDCYRRFLKPGAEERHYVMVGRIVTGLLMVAAAGVT
                     YILDTARASFDLMLSVGAGTGLLYLLRWFWWRINAWCEIAAMVSSFAIAVGFQVASRA
                     GHGLPSWESLIITVLATTVVWVATAFVTAPTERATLVNFYKLVRPAGAGWGTIPQEAG
                     VGGSPDSLAQSLLGWVLGCAFIYSALFGVGSALYGRLPQALVWAVVFVVSGVGLWRIL
                     PRMWTARA"
     gene            complement(889..2448)
                     /locus_tag="AC16JC_08880"
     CDS             complement(889..2448)
                     /locus_tag="AC16JC_08880"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArnT family glycosyltransferase"
                     /protein_id="HIR5164849.1"
                     /translation="MTQSTTNLDRLPPSSTVLHRHVLVALALATLILHVLANLVSPYG
                     FHRDEFLYFAMGRHLHLFRMDFPPAIAILAEAVRGTLGDGLFGIRLVPALCAALLALL
                     AGLFAWRLGGGRYAQFLAGLAVLGSPLFMRSGNLFQPVVLDQVAWTLALLALAQLAAA
                     GDGRWWLAIGAACGFGLLAKFSIAFIGFGIFLATLLTPLRRQLGTRWPWMGAALTLAI
                     GSPSIVGQIRLDFPIVLQMRGLSESQLEHVSPLGFVMTQLLFGPGTLLAVAAAVGFVV
                     VPALRPFRIVGWACLVVFLVLLGLRGKAYYFGPAYPALYAAGAVTLEHITRPRLGAWV
                     RWGTVVVIVAFGVFALPVGLPVLAPPAMARYAGNIGAARRNNQGELGRLPQDYADMLG
                     WEEEVRAVAQVYHALPAAERAEVVIVGDNYGEAGALEFYGPRYGLPAPVSAAGTYWQF
                     GPGDKPGTVVIVLGEEPQNLRDYVDSVVLAARVVNPWGVEEEQDVPIVVGRRPKTTLQ
                     ALWPSLGGRYR"
     gene            2831..4030
                     /locus_tag="AC16JC_08885"
     CDS             2831..4030
                     /locus_tag="AC16JC_08885"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000050 - urea cycle [Evidence IEA];
                     GO_process: GO:0000053 - obsolete argininosuccinate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HIR5164850.1"
                     /translation="MQTSHGPLALAFSGGLDTSYCVAKLAADGWTVHTVWVDTGGATD
                     ADRARIRAQAMAVGAARHHTVDAREQVYDRFVRFLIQGNVLRGEVYPLSVAAERTQQA
                     ISLVTVAREIGAAAVAHGSTGAGNDQIRFDVALRVLAPELKILTPIREAALTRPQAIA
                     WLEERHLPVPAQAGAYSINAGLWGTTWGGGWTHDPWTAPPAELLDPPADAPDPFEIVI
                     GWDQGLPMSLDGASLEGPALVERLGALTHRSGIGRNTHTGETALGIKGRIGFEAGAAL
                     VLIHAHREIEKLVLTRWQTFWKDHLGRFYGDRLHEGQYFDPALRDIEALIRSSQERVS
                     GETRVRLAPGRFQVTGVRSPFSLMDPAVATYGEEPRLWTGDEARAFARVSAIPALLAA
                     RAADASE"
     gene            4127..5224
                     /locus_tag="AC16JC_08890"
     CDS             4127..5224
                     /locus_tag="AC16JC_08890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013941658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164851.1"
                     /translation="MVVTTRIRLDRISSSTKNAALPREVIVGDAIVAEEGYILAVRIL
                     EDKSTYNTVEDTSGRMLALRAGDVLAGTLGERRALRGYAGVVPEHIAVGDVIHVLNLG
                     GILGICTSANPEIGPPFRAEVLGAVLAFPELGDRIGRPAHIRDHAIPPAERLDCTVPV
                     VYVAGTCMNAGKTAAATELVRGLSRSGLRVAAAKLTGVSLMRDALAMQDAGAAAALTF
                     NDAGAASTHAGITVPLAKAILNRLAAHPTGRPDVIVAELGDGILGEYGVQDILHDPEL
                     MAVAAVHVMCAPDPVACWGAAELYRNDFRLPITVISGPATDNEVGRKYVRDSLGLPAH
                     NALREAPALVGVVRDALLERVAVQPGSTAAA"
     gene            5221..>5856
                     /locus_tag="AC16JC_08895"
     CDS             5221..>5856
                     /locus_tag="AC16JC_08895"
                     /inference="COORDINATES: protein motif:HMM:NF013298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR5164852.1"
                     /translation="MKVAVIGAGGYAGGELLRLLAQHPAVTECVAASRSQAGRPWADV
                     HPALAAIGRSDATFLDAAPADAARGRDVVFLALEHGESSKVAGEIFSADPGLVVDLAA
                     DFRVRDTALYERYYGPHAAPALRSRFVYGLADVCGESLHGARAIAVPGCFATAAALAL
                     YPLARTDLAAAPPPALYAITGSLGAGVGPKPTTHPPARAHNVFAYGVLGHRH"
BASE COUNT          890 a         2092 c         2028 g          846 t
ORIGIN      
        1 gaactggggc acgtcctatc tcgtgcacga ctgctaccgg cgattcctca agccgggcgc
       61 cgaggagcgc cactacgtga tggtgggacg catcgtcacg gggctgctca tggtggccgc
      121 ggccggcgtc acctacatcc tcgacacggc gcgggccagc ttcgacctga tgctctcggt
      181 cggtgcgggg acggggctgc tctatctgtt gcgctggttc tggtggcgca tcaacgcgtg
      241 gtgcgagatc gccgccatgg tcagctcgtt cgccatcgcc gtcggattcc aggtcgcgtc
      301 gcgggccggc catggcctgc cctcgtggga atcgctgatc atcacggtgc tcgcgaccac
      361 ggtcgtctgg gtggcgaccg cgttcgtcac cgcgccgacc gagcgcgcga cgctggtcaa
      421 cttctacaag ctggtgcgtc ccgccggagc gggctggggt acgatcccgc aggaagcggg
      481 cgtcggcggc tcacccgaca gcctggcgca gtcgctgctg ggctgggtgc tggggtgcgc
      541 attcatctat tctgcgctgt tcggcgtggg gagcgcgctg tacggtcgcc tgccccaggc
      601 gctcgtctgg gcggtcgtgt tcgtggtcag cggggtcggg ctgtggcgta tcctgccacg
      661 catgtggacg gcacgggcct agcgacgatc taggaaagct ctgcgcaagt ccttgcgaac
      721 accgagggtg gggagctcgg acgcgttgga cgcgggggcg ctcgacccgg accaacgttt
      781 ccgatctcat tgccggccca gtagcgacca tgtccgggcc gtgcggcccc cgcggcctcg
      841 cttcctcgcc cctacccccg gagttcgccc cacttatgca gggctcccct agcggtaccg
      901 tcccccaaga gaaggccaga gggcctgcag cgtggtcttg ggtcgccgcc cgacgacgat
      961 cgggacgtcc tgctcctcct ccacgcccca ggggttcacg acccgcgccg ccagcaccac
     1021 cgaatccacg tagtcgcgca gattctgcgg ctcttcgccc aggacgatga ccacggtccc
     1081 aggcttgtcg ccgggcccga attgccagta cgtcccggcg gcactgaccg gcgccgggag
     1141 accgtaacgc ggtccgtaga actcaagcgc tcccgcttcc ccgtagttgt cgccgacgat
     1201 cactacctcg gcccgctcgg ccgccggcag cgcgtgatag acctgggcca cagcccgcac
     1261 ttcctcctcc catcccaaca tgtcggcgta gtcctgcggc aggcgtccga gctcaccctg
     1321 gttgttgcgc cgcgcggcgc cgatgttgcc ggcgtagcgc gccatggcgg gtggcgcgag
     1381 gaccgggagg ccgaccggca gcgcgaagac accgaaggcc acgatgacga cgaccgtccc
     1441 ccagcgcacc cacgcaccga gtcgcggccg ggtgatgtgc tccagcgtca cggcgccggc
     1501 ggcgtagagt gccgggtacg ccggtccgaa gtagtacgcc ttgccgcgca ggccgagcag
     1561 cacgaggaag acgaccaggc aggcccagcc gacgatccgg aagggccgca acgcgggtac
     1621 gacgacgaac ccgaccgctg ccgcgacggc gagtagtgtc ccagggccga acaggagctg
     1681 cgtcatcacg aaccccagcg gcgagacgtg ctcgagctgc gactccgaca gaccccgcat
     1741 ctggagcacg atcgggaaat cgagccggat ctgccccacg atgctcggcg acccgatcgc
     1801 cagggtcagc gccgcaccca tccagggcca gcgcgtgcca agttgcctgc ggaggggagt
     1861 cagcagggtc gcgaggaaga ttccgaaccc gatgaacgcg atgctgaact tggcgagcag
     1921 cccgaaaccg caggcggcgc cgatcgcgag ccaccagcgg ccgtcacccg cggcggcgag
     1981 ttgcgcgagc gccaggagcg ccagcgtcca ggcgacctga tccagcacca cgggctggaa
     2041 cagattcccc gatcgcatga agagcggaga gccgagtacc gccagtcccg ccaggaactg
     2101 cgcgtagcga ccgccgccca gccgccaggc gaagagcccg gcgagcagcg cgagtaaggc
     2161 ggcgcagagc gcaggcacga ggcggatgcc gaacaggccg tctcccagcg tgccccgcac
     2221 cgcttcggcc aggatggcga tggccggagg gaaatccatg cggaacaggt ggagatgccg
     2281 tcccatggcg aagtagagga actcgtcgcg atggaagccg tacggggaga cgagattcgc
     2341 gaggacgtgg aggatgagcg tcgctagggc gagcgcgacc aggacatgac ggtggaggac
     2401 ggtggaagac ggtggaagac ggtcgaggtt ggtggttgac tgagtcatcc tgctccaagc
     2461 gatcggtaga gctgccaggt gaggtagcgc gcgcggttct gttgatcgtc gagcgcgctc
     2521 caatcggcga cactgaaaag tccggattcc ttggcaaacc gcaaggcgta gccgacctcg
     2581 ggaagcgacg accttggtga cgcggtagac ggccagtgcc aactggtggc actcccgcca
     2641 ggccaggagc tcctcgtacg gggccatgat gggagcaacg tagtgcatac ccaccacctc
     2701 cccccatcat ttccatgctt cccggtaccg gaacgcgcac cacccccacc aacctccacc
     2761 gtcttccacc gccctcgacc gtccttctcc cccttgcata ttctccccct gaatcgcata
     2821 attatgcact atgcaaacta gccacggccc gctcgccctc gcgttttcgg gcgggctcga
     2881 cacgtcgtac tgcgtcgcca agctcgccgc cgacggctgg acggtgcata ccgtctgggt
     2941 cgataccggc ggcgccaccg acgccgaccg cgcgcgcatc cgtgcgcagg ccatggccgt
     3001 gggcgccgcc cgccaccaca cggtggacgc ccgcgagcag gtctacgacc gcttcgtccg
     3061 cttcctcatc cagggcaacg tgctgcgcgg tgaggtctat ccactgagcg tcgccgccga
     3121 acgcacccag caggcgatct cgctggtgac cgtggcgcgc gagatcggcg ccgccgccgt
     3181 cgcccacggc tcgaccggcg ccggcaacga ccagatccgc ttcgacgtgg cgctgcgtgt
     3241 cctggcgccc gagctcaaga tcctgacccc cattcgcgag gccgcgctga cccgtcctca
     3301 ggccatcgcc tggctcgagg agcgccacct cccggtgccg gcccaggcgg gcgcctactc
     3361 gatcaatgcg gggctctggg gtacgacctg gggcggcggc tggacgcacg atccctggac
     3421 ggctccgccc gccgagctgc tcgatccgcc ggccgacgct cccgacccgt tcgagatcgt
     3481 catcgggtgg gaccaggggc tgccgatgtc gctggacggc gcctcgctcg agggccccgc
     3541 cctagtcgag cggctcggcg ccctcacgca ccgctccggc atcggccgca acacgcacac
     3601 cggcgagacg gcgctcggca tcaagggccg catcggcttc gaggcgggcg ccgcgctcgt
     3661 gctgatccac gcgcaccgcg agatcgagaa gctggtgctc acccgctggc agacgttctg
     3721 gaaggaccac ctgggccgct tctacggcga ccggctgcac gaagggcagt acttcgatcc
     3781 ggcgctgcgc gacatcgagg cgttgatcag aagctcgcag gagcgcgttt cgggggagac
     3841 gcgcgtgcgg ctggcaccgg gccggtttca ggtgaccggc gtccgcagtc cgttttccct
     3901 catggatccg gcagtcgcca cctacggcga agagccccgg ctctggaccg gagacgaggc
     3961 gcgggcgttc gcgagggtgt cggcgattcc cgccttgctt gcggcacgcg cggcagatgc
     4021 aagtgagtga ctgcacggag cacggagcac agagcggtac tctccgtcgc gccgggaggt
     4081 actgctccct gctccgtgct ccccgctcct cagaggaggc cccagcatgg tcgtaactac
     4141 caggattcgg ctggaccgca tttccagcag caccaagaac gccgccttgc cccgcgaagt
     4201 gatcgtgggc gacgccatcg tggcggagga gggctacatc ctggccgtcc ggatcctcga
     4261 ggacaagagc acctacaaca ccgtcgagga cacgagcggc cgcatgctcg cgttgcgggc
     4321 gggcgacgtg ctcgccggca ccctggggga gcggcgcgcg ctgcgcggct acgcgggcgt
     4381 ggtgcccgag cacatcgccg tcggcgacgt catccacgtg ctcaacctgg gcggcatcct
     4441 cgggatctgc acctcggcaa acccggaaat cggcccgccg ttcagggcgg aggtgctggg
     4501 ggcggtgctc gccttccccg agctgggcga ccggattggg cggccggcgc acatccgcga
     4561 tcacgccatt cctcccgccg agcggctgga ctgcaccgtg ccggtggtgt acgtcgccgg
     4621 cacgtgcatg aacgcgggga aaacggcggc ggccacggag ctggtgcgcg ggctgtcgcg
     4681 cagcggactg cgcgtggcgg cggcgaagct caccggcgtc tccctgatgc gcgacgcgct
     4741 ggcgatgcag gacgccggcg cggcggcggc gttgaccttc aacgacgccg gcgccgccag
     4801 cacgcacgcc gggatcaccg tgccgctcgc caaggcgatc ctcaaccgcc tcgcggcgca
     4861 tcccaccggt cgtcccgatg tgatcgtggc cgagctgggt gacggcatcc tgggcgaata
     4921 cggcgtgcag gacatcctgc acgatccgga gctgatggcc gtggcggcgg tccacgtgat
     4981 gtgcgccccg gacccggtcg cctgctgggg ggcggccgag ctgtacagga acgacttccg
     5041 gctcccgatc accgtgatca gcgggcccgc gaccgacaac gaggtcggcc gcaagtacgt
     5101 gcgcgactcg ctcgggctcc cggcccacaa cgcgctgcgc gaggcgcccg ccctggtcgg
     5161 cgtggtgcgc gacgcgctgc tcgagcgcgt ggcggtccag cccggttcga cggccgcggc
     5221 atgaaggtcg cggtgatcgg cgccggcgga tacgccggcg gtgagctgct gcggctgctc
     5281 gcgcagcacc ccgccgtgac cgagtgtgtg gcggcgagcc gcagccaggc gggccgcccc
     5341 tgggccgacg tccacccggc cctggccgcg atcgggagga gcgacgcgac gttcctggac
     5401 gccgccccgg ccgacgcggc gcggggacgg gacgtcgtct ttctcgcgct cgagcacgga
     5461 gaatcctcca aggtcgcggg ggagatcttc tcggccgatc ccgggctcgt agtcgatctg
     5521 gccgccgact ttcgcgtgcg cgatacggcg ctgtacgagc gctactacgg cccgcacgcc
     5581 gcccccgccc tgcgctcgcg cttcgtctac ggcctggccg acgtgtgcgg ggagtcgttg
     5641 cacggcgccc gcgccattgc ggtgcccggg tgcttcgcca ccgccgcggc gctcgcgctg
     5701 tacccgctcg cccgcaccga ccttgcggcc gccccccccc cggccctgta cgccatcacc
     5761 ggcagcttgg gcgccggggt cggtcccaag cccacgacac accctcccgc acgcgcccat
     5821 aacgtgttcg cctacggcgt gctgggtcac cgccac
//
LOCUS       DBNGZW010000379        11659 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_514098, whole genome shotgun sequence.
ACCESSION   DBNGZW010000379 DBNGZW010000000
VERSION     DBNGZW010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 11659)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11659)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11659
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_514098"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="AC16JC_08900"
     CDS             complement(<1..218)
                     /locus_tag="AC16JC_08900"
                     /inference="COORDINATES: protein motif:HMM:NF045177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164853.1"
                     /translation="MTGTSLETHSAPAAASGVPGVELLAPQVADGGLVLTPAALSFVA
                     RLARVFEDRRRELLAARTARQRRILDGER"
     gene            802..1017
                     /locus_tag="AC16JC_08905"
     CDS             802..1017
                     /locus_tag="AC16JC_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164854.1"
                     /translation="MGAGRRSGNDRRALERRLSSDRRQQVVEVAVDRRSGLDRRMVLD
                     RRTGLDRRVIADRRTSVRPPAREPTRV"
     gene            1108..1752
                     /locus_tag="AC16JC_08910"
     CDS             1108..1752
                     /locus_tag="AC16JC_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164855.1"
                     /translation="MAVLTVVAMVCAVFGALLLAGMVIYARRRLLLRSIGLLLCGLFL
                     FALAALGGTVSVGVRGYRALTAEEVAARVRTEPMGPQRFRATVELPDGRVGQFDVAGD
                     QLYVDAHILKWKPVVNLLGLRTGYELDRVGGRYVAVADERQRPHTVFPLGPDRPVDVF
                     GFVRRHPRVLAPLVDATYGSGTFVGADQPGWFEIRVSTTGLLIRPLSDSTPGPR"
     gene            1768..2289
                     /locus_tag="AC16JC_08915"
     CDS             1768..2289
                     /locus_tag="AC16JC_08915"
                     /inference="COORDINATES: protein motif:HMM:TIGR03546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03546 family protein"
                     /protein_id="HIR5164856.1"
                     /translation="MVILKLLQSLVKALHSEGTPGQVAAGMALGSILGLTPLISLHNL
                     VVAALILLLNVSVPGATLGWALAIPLGFALDPMFDALGRQLLLDTSALVPLWTALANL
                     PVVPLSNFNNTVLLGSLVTALVLFAPIFFLSRWGVRRYRETIGERVQRSPLYRALTAS
                     RLYAVYRMFRPGT"
     gene            2291..4054
                     /locus_tag="AC16JC_08920"
     CDS             2291..4054
                     /locus_tag="AC16JC_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR03545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03545 family protein"
                     /protein_id="HIR5164857.1"
                     /translation="MKVAIFRWKAIVPLALLLALLVGVWLLFADRFGRLAVERGGTAI
                     LGARVDVGSFHLSLGEGKVTVRHLVAASPFDSLRNLVEADELTADIDLLPLLEKKVAI
                     DRLAANGLRFGTPRPTSGFVRRGGSGAASQIMANVRSWAAQPALQVPILQLATGQLKV
                     DSLDPRKLQTVQAAESLAARADSSGRAWSGALDSLDARGTADSALAFAKRLQGARPTD
                     LALLRDGRRMLDRVKQTRDRLGALERGVKAGVGTLDQGLASLDAARRRDYDFARSLVR
                     LPPIDATAIGWVLFGPEAVHKFQRALYYTELGRAYMPPGLLPRAHPSPKRRAGVTVEF
                     PRANRLPAFLLRDAELSFLLGSDSGGLRRYAGRLQGLTSAPALYGRPTTLEVRAPTVS
                     ADGLFDHVRATPRDAVGATVRGVTLPAITLPGLPLRVNPGQGDVSLGFALYGDSLRGR
                     WTVSAPAAVWQRDSGAAGGLAADLLWRVVSGIENLDLTAELGGTLSHPELHVRSNLDR
                     AVSDRLRSMIGAEVDAAESRLRVAVDRFADEKVTPVKAQVAALTAQVNERVQQGRQAL
                     DAAQQQIEQQLKRLTGGIRLP"
     gene            4144..4707
                     /locus_tag="AC16JC_08925"
     CDS             4144..4707
                     /locus_tag="AC16JC_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5164858.1"
                     /translation="MATLALALSDAVPAPVADDLVRRAQAGDEAAFEALYRVHVGRVF
                     ALCLRLSGDRVRAADLAQDVFVRCWERLRSFRGESAFGSWLYRLAVNLVWMANRGDRR
                     REARVLPVEDPQPLERPAESSPVALSVDLERAIGSLPDGAREVFVLFDIDGYHHEEIA
                     RMTGIAVGTSKAQLFRARRLLREWLER"
     gene            4704..5738
                     /locus_tag="AC16JC_08930"
     CDS             4704..5738
                     /locus_tag="AC16JC_08930"
                     /inference="COORDINATES: protein motif:HMM:NF016648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR domain-containing protein"
                     /protein_id="HIR5164859.1"
                     /translation="MNERERDVELPPELRQAVDNLPRAIEPPGDLWAGIKERIRQGKG
                     EGGRGKGPLPTRWWIPLAAAAALVLALGVRFWLRQAGAWEVQRTAGVPRVDDAPLASR
                     GTLHVGDWVETDDTSQAVIAVGAIGHVDVRPGSRVRLVRARDTDHRLALAYGAIHAKV
                     DAPPRIFFVDTPAGTAVDLGCEYTLETDRNGRGLIHVTGGYVELAWGGVRSVVPLGAY
                     AVTRPDEGPGTPFVEDAPAALREALAAFDFERGGADAARRALAAARAEDALSLWHLLT
                     RVDAGLRGEVYDRLAALVPPPPGVRRTSAVVGDTTALERYWSLIRRIEFRRVILRGIR
                     DIDPRTGLSR"
     gene            complement(5766..8798)
                     /locus_tag="AC16JC_08935"
     CDS             complement(5766..8798)
                     /locus_tag="AC16JC_08935"
                     /inference="COORDINATES: protein motif:HMM:NF016345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic secretory protein-like protein"
                     /protein_id="HIR5164860.1"
                     /translation="MNAGRLVVVAAVVGLLAPVAASGQDFGRNKVQYESFDFQVLRTE
                     HFQVYFYPEEREAAQQAARMAERWYARYAALLGHQLRGAQPLVLYASHPHFEQTNVVS
                     GELDEGTGGVTESLKRRIVLPLAGPLAETDHVIGHELVHAFQYDITGTGRGGATEAAS
                     RLPLWFIEGMAEYLSLGPVDPNTAMWLRDAVRHNKIPSIRQLEDPRYFPYRWGQAFWA
                     YLTSRYGDAAVATLLKSAGKSGNVTVALEQISGEKLDSLSRQWQQAVRDTYTPVEGAT
                     ATPETFGRALASAKSAAQLNVAPALSPDGSRIAFFSERGLFSIELYLADVASGRVERT
                     LTHTALDAHYESVGFISSAGAWDATGRRFAFGVVEHGRPLLSILDVSSGKPEREIPFP
                     SLGEIFNTTWSPDGKAVAFSAQVNGWTDLYVYDLQRSVLRRLTNDLYADLQPAWSPDG
                     RSLAFVTDRFTTDTADLAAGPYRLALLDLSTGAIRPLPAFPGAKHINPQWNPDGQSLY
                     FVSDRGGIDNVYRLQLSSGTTTQVTNLFAGASGITAVSPVISVAQRTGELVFTAYDST
                     GYRLYTVKDPAVLAGKPLATLPVTDAAALPLTRRASGADQVAAKLADARTGLPAAGTG
                     RVANYRAGLSLDYVAQPYLAVGASSFGTFVSGGATLVWSDMLGDHSLITGLQVNGGLK
                     DIGAVAAYINQHRRLNWGVAAQQTPYRVGTFDNASGTVKGQPAYIEITDIFRQTERSA
                     TTFASYPLSRVQRLEFSAGYENISFDAERRFLAFDPTGSFVLLDSTESLPTARALNLG
                     VGGAALVYDNSFFGATSPILGSRYRIEVDPMFGSLQTVTVLADYRRYIMPVRKLTFAG
                     RVVHAGRYGPDAQRLFPLYIGYQQLVRGYDIGSISASECPSNSATCPVVDRLQGSRIL
                     VGNVEARFPLLGVLGLGGGYYGALPIEAGVFADAGVAWNKADQPTFFDVRRQAVTSVG
                     AALRMNLFGFAIAELDYVKPFQRPEKGWYWQFGLTPGF"
     gene            9019..10383
                     /locus_tag="AC16JC_08940"
     CDS             9019..10383
                     /locus_tag="AC16JC_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5164861.1"
                     /translation="MPAVTPTYARRLGLFSATMAVIGGIIGSGIFATPNVVAQRVGSG
                     GLALAAWVLGGLVALAGAFCYAELAERMPRAGGQYVYLRDAIGPLPAFLYGWALLLVM
                     ATGAAAFVAVTFANYTISLLGLAAAAKLPLAMGAIVVVCAINYVGVTPGAITQNVFTL
                     LKLGALTCLIAAGLLLPVHPTGADTAVRHAPLVVPGGGLAVTVALGAALVPVLFTYGG
                     WQQTNFVAEEIVDAERTLPRALVLGVLAVIAVYLLANVAYLVVLGPERLAASVAPAAD
                     AMERVIGPAGRVVISAGIAVSSFGFLDLVVLVTPRVFQAMAADGVFFPGLARLHPRYR
                     TPGAAIVVMGAWSAALTLTGTYGRLLDYVVFGDWIFFGSTVATLFIYRARERRRVGGM
                     AETRVRVPGYPWTPAFFVLAALYVVASSVGANPKNAAVGTALILLGVPVFLFWRARLR
                     TGTG"
     gene            10464..11159
                     /locus_tag="AC16JC_08945"
     CDS             10464..11159
                     /locus_tag="AC16JC_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164862.1"
                     /translation="MKRARLAVRVLAVGALWGCGDSSGPGAGVITAKVTDPAGDAFGA
                     NAVQWDLTAMTITRDSGGITILLDFSNSVISPASGDTTAMLGFVDLDVDQDSTTGLGT
                     VVDDFRPGSGSTGMGSEYLVDLSTATAAVFDTLRASTGPVTATYSGKRVTIRVPRSRL
                     GNDDAFLNAAAIVGTRAEPTDIIPENGHLKVGGTGMVAPYQPRVSALRTGPLARRVWA
                     PGVSSRRRNDGGR"
     gene            complement(11156..>11659)
                     /locus_tag="AC16JC_08950"
     CDS             complement(11156..>11659)
                     /locus_tag="AC16JC_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR5164863.1"
                     /translation="IPFSPQNTEILRQRFRNVTVSTIIGTGLVAAIQGAMVGLAFWAA
                     GLSNALFWGVVTMVLAILPVVGSGLVFGPGALALALGGRWGWAIAMALWGLLVVGNVD
                     YVVRPIIFRRWANIHPLITLIGALAGVPYFGLLGLLIGPLALSYFFELIRMYREEYLA
                     GQTAGVA"
BASE COUNT         1646 a         3861 c         4290 g         1862 t
ORIGIN      
        1 cgctcgccgt ccaggatgcg acgctggcgc gcggtccgcg cggccagcag ctcgcgtcgg
       61 cggtcctcga agacccgggc gagccgtgcc acgaacgaca gcgcggccgg cgtgagcacg
      121 aggcccccat cggcgacctg gggagcgagc aactcgacgc ctggaacgcc agacgccgcg
      181 gcgggggcag aatgtgtctc gagtgaagta ccggtcatgg gtgacgggcg gcagggtggc
      241 aagtgtcatg cctctgggct gcgttgctcg ggcgagacgt ccccggttcg gaggtgattt
      301 agcatacccg atgttgtggt gagggtgcca catggagagg ttgaaacgcg aatgaatgtc
      361 tctcctgtca cgctccgagg ccgtcacgtc gaattgcgcc ctgtttctgt gcacgacgtc
      421 gcggcgttga gcgcgtgaga ctggacgagg agcgttgacg cacgcgtcga aaccgtgggc
      481 tgcgcgcggg tggagttcaa gaccgaccgc ctcaactcga tctacttcgg cagcctggcc
      541 gaggaatggc cgggcgtgcg cggggcgctc gaggggaagc tgcggacctg aacgatccgc
      601 tgtcgctttc gcacgtcacc cgtggcggag gggccatgac acggccggag tgtggccgat
      661 gaccggcggg ccaagtgcct gatgccgcac gagctagcgg ccggaagcgg gccccaagtg
      721 gcggctaacc aaaggcatag gtcttgcgaa gctggcgggt tcgaggagaa ctcctagaga
      781 ggttttgcga gggaggggcg tatgggtgcg ggccggcgaa gcggcaacga tcgacgggcg
      841 ctcgagcggc gcctgagcag tgatcggcga cagcaggtcg tcgaggtagc ggtcgaccgc
      901 cgtagcgggc tcgatcgccg catggtgctc gatcgtcgta cggggctgga tcggcgcgtg
      961 attgccgacc gccgcacgag cgtgcgtcct cccgcgcgcg agccgacgcg ggtgtaagtc
     1021 tctcgccgat cggtccaggg cgccggccct ctccgtgatc cggggagggc ctttgcgttc
     1081 ccgggaccgg tagtagctta ccgctcgatg gctgtgctca cggtcgtcgc catggtgtgc
     1141 gcggtatttg gcgcgctgct cctcgccggc atggtcatct atgcccgccg gcgcctgctg
     1201 cttcgcagca taggcctcct gctctgcgga cttttccttt tcgcgctggc cgccctcggg
     1261 ggcacggtga gcgtgggagt caggggttac cgggccctga ccgccgagga ggtggccgcc
     1321 cgggtgcgaa ccgagccgat gggcccccag cgcttccgcg ccacggtcga gctccccgac
     1381 ggccgggtgg gacagttcga cgtcgcgggc gaccagctct atgtggacgc ccacatcctc
     1441 aagtggaagc cggtcgtcaa cctgctcggg ttgcgcaccg gctacgagct ggaccgggtg
     1501 ggcgggcgtt acgtcgcggt cgccgacgag cgccaacgtc cccataccgt ctttcccctc
     1561 ggccccgacc ggcccgtgga cgtgttcggg ttcgtgcgcc gccacccgcg agtgctcgca
     1621 ccgctggtgg acgccacgta cggttcgggg acgttcgtgg gcgccgatca accgggatgg
     1681 ttcgagatcc gcgtgtccac gacggggctg ctgatccggc cgctgtccga ctcgacgccc
     1741 ggcccccgct agcctgactg gaggctcatg gtcatcctca agctgcttca gtcgctggtg
     1801 aaggcgctgc acagtgaggg gacgccgggg caggtggccg cggggatggc cctgggctcg
     1861 attctcggtc tcacgccgct catcagcctg cacaacctcg tggtggccgc cctgattctc
     1921 ctgctgaacg tgtccgttcc cggcgctacg ctggggtggg cgctcgccat cccgctggga
     1981 ttcgccctcg acccgatgtt cgatgcgctg ggacggcagc tgctcctcga tacctccgcg
     2041 ctcgttcccc tgtggacggc gctggcgaac ctgccggtcg tgccgctgag caatttcaac
     2101 aacaccgtgc tgctcggcag cctcgtgacc gcgctggttc tgttcgcccc gatcttcttc
     2161 ctcagtcgct ggggagtgcg ccgctaccgc gaaaccatcg gggaacgggt gcagcgctcc
     2221 ccgctgtacc gggcgttgac cgcctcgcgg ctctacgcgg tgtaccgcat gttccggccg
     2281 gggacgtgac atgaaggtcg cgatcttccg ctggaaggcg atcgtgccgc tggcgctgtt
     2341 gctcgcgctg ctggtggggg tatggctctt gttcgccgac cggttcggcc gcctggccgt
     2401 ggagcgaggc ggcacggcca tcctcggcgc gcgggtggac gtgggatcgt tccatctatc
     2461 ccttggcgag ggcaaggtca ccgtccgcca cctcgtggct gcgtctccct tcgattccct
     2521 ccggaacctg gtggaagcgg acgagctgac ggccgacatc gatctcctgc cgctgctcga
     2581 aaagaaggtc gcgatcgacc gcctggccgc caacgggctg cgcttcggga ctccgcgccc
     2641 caccagcggg ttcgtgcgcc gtggcgggtc gggcgcggcg tcgcagatca tggccaatgt
     2701 gagatcctgg gcggcgcaac cggcgttgca ggtgccgatc ctgcagctcg cgacggggca
     2761 gctgaaggtg gacagcctcg acccccgcaa gctccagacg gtgcaggcgg ccgagtcgct
     2821 ggccgcgcgg gccgattcga gcgggcgcgc ctggtcgggt gccctggaca gtctcgatgc
     2881 ccggggcacg gccgattccg cgctcgcgtt cgcgaagcgc cttcagggcg cacgtcccac
     2941 cgacctcgcg ctgctgcggg acggccggcg catgctggac cgcgtgaagc agacgcgcga
     3001 ccgcctgggc gcgctcgaac ggggagtgaa agcgggcgtg ggcacgctcg accagggtct
     3061 ggcgtcgctc gatgccgcgc ggcggcgcga ctacgacttc gcccggagcc tcgtgcggct
     3121 gccgccgatc gatgcgacgg cgatcggctg ggtgctcttc ggcccggagg cggtgcacaa
     3181 gttccagcgg gccctctatt acacggagct gggacgggcg tacatgcccc ccggcctcct
     3241 gccgcgtgcc catccgtcac ccaagcgtcg cgcgggcgtg acggtcgagt tcccgcgcgc
     3301 caaccgcctg ccggcattcc tgctgcgcga cgccgagctt tcctttctgc tgggaagcga
     3361 ttccggcggc ttgcggcgct acgccggccg gttgcagggg ctcaccagcg cgcccgcgct
     3421 gtacggtcga cccaccacgc tcgaagtgcg ggcgccgacc gtttcggcgg atgggctgtt
     3481 cgatcatgtg cgtgcgacgc cgcgcgacgc ggtgggcgcc acggtgcgcg gtgtcacgct
     3541 gcccgccatc acgctccccg gcctgccgct gcgggtgaac ccggggcagg gcgacgtgtc
     3601 gctcggcttc gcgttgtacg gcgattcact gcgcggccgc tggaccgtga gcgctcccgc
     3661 cgccgtatgg cagcgcgaca gcggcgctgc cggtggcctc gccgccgatc tgctgtggcg
     3721 cgtcgtctcc ggcatcgaga atctcgatct cacggccgag ctggggggca cactgtcgca
     3781 ccccgagctg cacgtgcgat ccaatctcga ccgcgcggtg tccgaccgcc tgcgctcgat
     3841 gatcggggcc gaagtggacg ccgcggagtc gcggctgcgc gtcgcggtgg accggttcgc
     3901 ggacgagaaa gtcaccccgg tgaaagccca ggtggccgcg ctcaccgcgc aggtgaacga
     3961 acgggtccag caggggcgtc aggcgctgga cgcggcgcag cagcagatcg agcaacagct
     4021 gaagcggctg acgggaggga tcaggctgcc ctgagggagg acggtggagg gcggtggagg
     4081 ttggtggagg tggtggaggt ggtccgtttc aaacccgaac cacatttccg gcatcacact
     4141 catgtggcca cgctcgctct cgcgttgtcg gacgccgtgc cggcgccggt agcggacgat
     4201 ctggtccggc gcgcccaggc gggcgacgag gccgcgttcg aggcgctgta ccgcgtccac
     4261 gtgggccgcg tcttcgcgct gtgcctgcgg ctctcgggcg accgcgtccg ggcggccgac
     4321 ctcgcgcagg acgtgttcgt gcgctgctgg gagcggttga ggagtttccg cggtgagagc
     4381 gcgttcggct cctggctgta ccggctggcg gtgaatctcg tgtggatggc gaaccgtggc
     4441 gatcggcggc gtgaagcgcg cgttctgccc gtggaagatc cgcagccgct cgagcggccg
     4501 gccgagtcgt cgccggtggc cctcagcgtc gatctggagc gcgccatcgg gtcgttaccc
     4561 gacggggcgc gggaggtctt cgtgctgttc gacatcgacg gctaccacca cgaggagatc
     4621 gcccggatga cggggatcgc cgtggggaca tccaaggcac agctgttccg ggcccggcgg
     4681 ctgctgcggg agtggttgga gcggtgaacg agcgcgagcg ggacgtggag ctcccaccgg
     4741 agctgcggca ggcggtggac aacctgccgc gggcgatcga gccaccgggg gatctgtggg
     4801 ccgggatcaa ggagcggatc aggcagggga agggggaagg gggaagaggg aagggacccc
     4861 tccccacccg gtggtggatt ccgctcgcgg cagcggcggc gctggtgctg gcgctcggcg
     4921 ttcgcttctg gctgcggcag gcaggtgcgt gggaggtgca acggaccgcg ggcgtgccgc
     4981 gcgtggacga tgcgccgctc gcgtcacgcg gcacgctgca cgtcggtgac tgggtggaga
     5041 ccgacgacac gtcccaggcg gtcatcgcgg tgggcgcgat cggccacgtg gatgtgcgtc
     5101 ccgggtcgcg cgtgcggctg gtgcgggcgc gcgatacgga tcaccggctg gccctggcgt
     5161 acggagcgat ccacgccaag gtggacgcgc cgccgcggat cttcttcgtc gacacgcccg
     5221 cgggcactgc cgtcgatctc ggctgcgagt acacgctgga aaccgaccgg aacggacgcg
     5281 ggttgatcca cgtgacgggc ggctacgtgg agctcgcctg gggcggagtg cgctcggtcg
     5341 tgccgctcgg cgcctacgcg gtgacgcgtc cggacgaagg accgggcact ccgttcgtcg
     5401 aggatgcgcc ggcggcgttg cgcgaggcgc tcgcggcgtt cgatttcgag cgcggtgggg
     5461 cggatgcggc gcgccgggcg ctggccgcgg cgcgcgccga ggacgccctc tccctgtggc
     5521 atctgctgac gcgcgtcgat gccggcctgc gtggagaggt gtacgaccgc ctggcggcgc
     5581 tcgttcctcc accgccaggg gtcagacgca cgagcgcggt cgtgggcgac acgaccgcgc
     5641 tggagcgcta ctggagtctc atccggcgga tcgagttccg ccgcgtgatc ctgcgcggca
     5701 tccgggacat cgatccgcgc acggggctgt cgcgctgagg gaggttatgg aaacctcctt
     5761 cagtttcaga atcccggcgt caggccgaac tgccagtacc agcccttctc gggtcgctgg
     5821 aacggcttca cgtaatccag ctcggcgatc gcgaagccga agagattcat gcgcagcgcc
     5881 gcccccacgc tggtgaccgc ctggcgccgg acgtcgaaga aggtcggctg gtcggcttta
     5941 ttccaggcca cgccggcgtc ggcgaagaca cctgcctcga tcggcagggc gccgtagtag
     6001 ccaccgccga gccccagcac gccgagaagc gggaatcggg cctcgacgtt gccaaccaga
     6061 atgcggctgc cttggaggcg gtcgaccacc gggcaggtgg cactgttgga ggggcactcg
     6121 gatgcgctaa tcgagccgat gtcgtagccg cgcaccagct gctgatagcc gatgtaaaga
     6181 gggaacagcc gctgagcgtc cggtccatac cggcccgcgt gcacgacgcg cccggcgaac
     6241 gtcagtttgc gtaccggcat gatgtagcgc ctgtaatccg ccagaaccgt gaccgtctgc
     6301 agcgagccga acatcggatc cacttcaatc cggtatcgcg agccgaggat ggggctggtc
     6361 gccccgaaaa atgagttgtc gtacacgagc gccgctcccc cgacacccag gttgagcgct
     6421 cttgcagtcg gcagggactc cgtcgaatcg aggaggacga agctgccggt cggatcgaag
     6481 gcgaggaacc gccgctcggc gtcgaaggag atgttctcgt agccggccga gaactcgagc
     6541 cgctgcaccc ggctcagggg atagctcgcg aaggtggtcg cggaccgttc ggtctgccgg
     6601 aatatgtccg tgatctcgat gtaggcgggc tgccctttca cagtcccgga cgcgttgtcg
     6661 aaggtcccca cgcggtaggg ggtctgctgg gccgcgaccc cccagttcag gcggcgatgc
     6721 tggttgatgt aggcggccac cgcgccgatg tccttgaggc cgccgttcac ctgcagcccc
     6781 gtgatcaggc tgtggtctcc cagcatgtcg ctccagacca gggtcgcgcc gccgctcaca
     6841 aacgtgccga agctcgaagc gccgacggcg agatacggct gggcgacgta gtcgagcgac
     6901 agacccgccc ggtagttcgc gacccgaccg gttccggcgg ccggcaaccc ggtccgggcg
     6961 tccgcgagct tcgccgcgac ctgatccgct ccggacgccc ggcgcgtcag cggcagggcg
     7021 gccgcatcgg tcaccggcag cgtcgcgagc ggcttgccgg ccaggacggc cgggtccttc
     7081 accgtgtaca gccggtaacc ggtcgagtcg taggccgtga agaccagctc gcccgtgcgc
     7141 tgcgccaccg agatgaccgg gctcacggcc gtgatgccgc tcgcgccggc gaacagattg
     7201 gtgacctgag tggtcgttcc ggacgagagc tgcagccggt acacgttgtc gatgccgccg
     7261 cggtccgaga cgaagtagag gctctgcccg tccgggttcc actgcgggtt gatgtgcttg
     7321 gctcccggaa aggcggggag cggcctgatc gctcccgtgg agagatcgag cagcgccagc
     7381 cggtacggtc ccgcggcgag atcggccgtg tcggtcgtga agcggtcggt gacgaaggcc
     7441 agcgaccggc cgtcgggcga ccaggcgggc tggagatcgg cgtacagatc gttcgtgagg
     7501 cgccgcagca cgctgcgctg caggtcgtac acgtagagat cggtccaccc gttcacctgc
     7561 gccgagaacg ccacggcctt gccgtcgggt gaccaggtgg tgttgaagat ctcacccagg
     7621 gaggggaagg ggatctcccg ctcgggtttg ccggagctca cgtccagaat cgacagcagc
     7681 gggcgcccgt gctccacgac gccgaaggcg aagcggcgtc cggtcgcatc ccaggcgccg
     7741 gcggagctga tgaacccgac gctctcgtag tgcgcgtcga gcgccgtgtg ggtgagggtc
     7801 cgttccaccc ggccgctcgc tacatcggcg aggtacagct cgatcgagaa caggccacgc
     7861 tcggaaaaga aggcgatgcg actgccgtcg gggctcaggg ccggcgccac gttcagctgc
     7921 gccgcgctct tcgccgacgc cagggcccgg ccgaacgtct ccggagtcgc cgtcgcccct
     7981 tcgacgggcg tgtaggtgtc gcgcacggcc tgctgccact ggcgggagag cgaatccagc
     8041 ttctcgccgg agatctgctc cagcgcgacg gtgacgttgc cgctcttgcc ggcgctcttc
     8101 agcagcgtgg cgacggcggc gtccccgtag cggctcgtca ggtacgccca gaacgcctgg
     8161 ccccagcgat aggggaagta gcgcggatcc tcgagctggc ggatcgaggg aatcttgttg
     8221 tgtcggaccg cgtcccgcag ccacatcgcg gtgttcgggt ccacggggcc gagcgagagg
     8281 tactccgcca tgccctcgat gaaccagagc ggcaaccggg aggccgcttc cgtcgctccg
     8341 ccccgcccgg tgcccgtgat atcgtactgg aaggcgtgga ccagctcgtg gccgatgacg
     8401 tgatcggtct cggcgagggg acccgcgagc ggcagcacga tgcgccgctt caggctctcc
     8461 gtgacgccgc cggtcccctc gtcgagctcg ccggacacga cgttcgtctg ctcgaagtgg
     8521 gggtgactcg cgtacagcac gagcggctgg gcgccccgca gctggtggcc cagcagggcg
     8581 gcgtagcgtg cgtaccagcg ttccgccatc cgggctgcct gctgcgcggc ctcgcgctcc
     8641 tccgggtaga agtacacctg gaagtgctcg gtccggagca cctggaagtc gaacgactcg
     8701 tactggacct tgttgcgccc gaagtcctgc ccggaggcgg cgacgggggc caggagcccc
     8761 acgacggcag ccaccactac caatcgaccg gcgttcacgg taccacctcg ctgtgcggtg
     8821 cggatgcgac tgcagtgcct tctactacca ccacgttgta ctacgaagcg tgtgaggagt
     8881 tcgaaggttc catgaccgcg gcgcgccccg agcgtgcgcg cggaccgcga caccggtatc
     8941 tgacagcagc cggacgatcc cccccgcacg ggcaggcccg gggtcgtcaa cggtcgcgac
     9001 ggcggatacc atatccgcgt gcccgccgtc actcccacct acgcccgccg gctcggtctc
     9061 ttctccgcca ccatggcggt gatcggcggc atcatcgggt cggggatctt cgcgacgccc
     9121 aacgtggtcg cccagcgcgt ggggtcgggc ggcctggcgc tggccgcctg ggtgctgggc
     9181 ggtctcgtgg ccctcgccgg ggcattctgc tacgcggagc tggcggagcg gatgccgcgg
     9241 gccggcgggc agtacgtcta cctgcgggac gcgatcggtc cgttgccggc cttcctgtac
     9301 gggtgggcgt tgctgctcgt gatggcgacc ggggccgcgg cgttcgtcgc ggtgaccttc
     9361 gccaactaca ccatcagcct gctgggcctc gccgcggcgg cgaagctgcc gctcgccatg
     9421 ggagcgatcg tggtggtctg cgcgatcaac tacgtggggg tgacgcccgg ggcgatcacg
     9481 cagaacgtct tcacactctt gaaactcggc gccctcacgt gcctgatcgc cgcggggttg
     9541 ctgctcccgg tgcatcccac gggggccgat acggcggtgc gtcacgcgcc gctcgtggtt
     9601 cccggcggag gactggccgt gacggtcgcg ctgggggcgg cgctcgtccc ggtgctgttc
     9661 acttacggcg ggtggcagca gaccaatttc gtggccgagg agatcgtgga cgccgagcgc
     9721 accctgccgc gggcgctggt gctgggcgtg ctggcggtga tcgcggtcta tctgctggcg
     9781 aacgtcgcct acctggtcgt gctcggtccc gagcgcctgg cggcgagcgt ggcgccggcg
     9841 gcggacgcga tggagcgcgt gatcggcccg gccggacggg tggtgatcag cgccgggatc
     9901 gccgtgtcga gcttcgggtt cctcgacctc gtggtgctgg tgacgccgcg ggtgttccag
     9961 gccatggcgg cggacggggt gttcttcccg ggactggcgc ggctgcatcc gcgctatcgc
    10021 acgccgggtg cggcgatcgt ggtgatgggc gcgtggtcgg cggcattgac cctcaccggg
    10081 acgtacggca ggctgctcga ctacgtggtg ttcggggact ggatcttctt cgggtccacg
    10141 gtggcgacgc tgttcatcta tcgtgcgcgc gaacggcggc gcgtgggcgg catggccgag
    10201 acccgggtgc gtgtgccggg ctacccctgg acgccggcgt tcttcgtcct ggccgcgctg
    10261 tacgtcgtgg ccagctcggt gggggcgaat ccgaagaacg ccgcggttgg aaccgcgctc
    10321 atcctgctgg gcgtgccggt gttcctgttc tggagagcca gactacggac aggaaccgga
    10381 tgagcggccg tggcggccgg gtgatggcct cgcgccggct tcgaggttac tcttggagcc
    10441 ctacgactcc cgaggatcgg accatgaagc gcgcgcggct cgccgtgagg gtcctggcag
    10501 tgggggcact ctggggatgc ggggattcct ccggcccggg agccggtgtg attaccgcga
    10561 aggtgaccga cccggcgggc gacgcgttcg gcgccaatgc cgtccagtgg gacctcaccg
    10621 ccatgacgat tacgcgcgac agcggcggca tcaccatcct gctcgacttc tcgaattccg
    10681 tgatctcgcc cgcgagcggc gatacgacgg cgatgctcgg cttcgtcgat ctcgacgtgg
    10741 accaggattc cacgaccggc ctgggtacgg tcgtcgacga cttccggccg ggcagcggct
    10801 cgacggggat gggctcggag tacctggtcg acctatcgac ggcgacggcg gcggtgttcg
    10861 acaccctgag ggcttccacg ggtccggtca cggcgaccta tagtggaaag cgcgtgacga
    10921 tccgcgtgcc ccggtcccgg ctcggcaacg acgatgcgtt tctgaacgcg gcggccatcg
    10981 tcggcacccg tgcggagccg accgatatca tccccgagaa cggccatctg aaggtgggcg
    11041 ggacgggcat ggtcgcgccg tatcagcctc gcgtctcggc gctgcggacc gggccgctcg
    11101 cgcggcgggt ctgggctcca ggcgttagct cgcggcggcg caacgacgga ggccgctagg
    11161 ccacgccggc cgtctgcccg gcgagatact cctcgcgata catccggatc agctcgaaga
    11221 agtagctcag cgccagcggc ccgatcagca ggcccagcag cccgaaatac ggcacaccgg
    11281 cgagcgcgcc gatcagcgtg atcagcgggt ggatgttcgc ccagcgacgg aagatgatcg
    11341 gacggaccac gtagtccacg ttcccgacga cgagcaggcc ccacagcgcc atggcgatgg
    11401 cccagcccca gcgcccgccc agcgccaggg cgagcgctcc cggtccgaag accagcccgc
    11461 tgcccaccac cggcaggatc gcgagcacca tcgtcaccac gccccagaag agcgcgttgg
    11521 agagccccgc ggcccagaag gcgagtccca ccatcgcgcc ctgaatcgcg gccaccaggc
    11581 cggtgccgat gatcgtggag accgtcacgt ttcggaagcg ctgccgcagg atctcggtgt
    11641 tctgcggcga gaacgggat
//
LOCUS       DBNGZW010000380         3484 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_707531, whole genome shotgun sequence.
ACCESSION   DBNGZW010000380 DBNGZW010000000
VERSION     DBNGZW010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_707531"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="AC16JC_08955"
     CDS             <1..1011
                     /locus_tag="AC16JC_08955"
                     /inference="COORDINATES: protein motif:HMM:NF039585.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5915 domain-containing protein"
                     /protein_id="HIR5164864.1"
                     /translation="AIIEFVVEDLSTWYVRLNRPRFWAPDRAADEAAVATLHEALVTT
                     ARLLAPAAPFLADWLHRSLLGTSVHLERFPQDRGRREPELLAAMAAVRCLASLARAAR
                     ESRSLRVRQPVARMQLAVPAAVRGPLLADLLDILVAEVNVKSYQVVESDDELVTLHGK
                     ANFRSLGRRYGKATPQAAAAVAGLAPHELHALERGEAVRSGEWEFLPEDVTVTRDVAS
                     EWIVQADGPYVVALDPSLTPDLEQEGLAREVVNRVQRLRKEAGYEYTTRIELSVSGAP
                     EVIAAVEAFEGYVAGETLARRMQLVGTLEDADITREVDIEGRAVTLALRRHDARKGGT
                     RE"
     gene            1008..1412
                     /locus_tag="AC16JC_08960"
     CDS             1008..1412
                     /locus_tag="AC16JC_08960"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR5164865.1"
                     /translation="MTTKTKGLNKKQLAHLEKRLLEERARVMKELGHYGESFNSSLQA
                     SDGDLSSYSFHMADQGTDAMEREKAFLFASQEGRYLWHVNQALRRLYQTPEKFGKCEQ
                     CGEEIGFDRLDALPHARLCINCKAKEEDGKRR"
     gene            1396..1920
                     /gene="lspA"
                     /locus_tag="AC16JC_08965"
     CDS             1396..1920
                     /gene="lspA"
                     /locus_tag="AC16JC_08965"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR5164866.1"
                     /translation="MASAASRRLFWGTIVGIVALDLLTKYLAEGVLARRPVPVIGDYV
                     VLRLVYNQCAAFGLCLGPPEYSRWIFLVLALLALTVLGSMVRHTPDHDRLRLFVLALL
                     VAGAIGNVLDRVRSAQGVVDFIEVGIGSYRWPTFNVADSAITVGAIALAVSLWLEGRA
                     RGSGAAKEAGTAPS"
     gene            1917..2933
                     /locus_tag="AC16JC_08970"
     CDS             1917..2933
                     /locus_tag="AC16JC_08970"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR5164867.1"
                     /translation="MTPDGTTFCIVVPVTERLDRFLADQLALSRTQAARLIADKRVSA
                     DGKPLRASALLERGAVVTVEFPPTAPPRTYAPAHTDLRFVFEDEHLAVLDKPAGLVVH
                     PGPGHWDDTLVNALVGRGTALSEGSTAGRPGIVHRLDRDTSGLLLVAKTDLAHRRLAR
                     AIERREVERVYACVTWGHFAKSPVDIEAPIARHIEDRKRMTVRAGGRHAVTHVEVVAR
                     FPLVDLLRVRLGTGRTHQIRVHVAHVGHPVVGDRDYAFGGSRRVTPSERRAAERLESL
                     APRQLLHAAMLSFRHPVTGDALTLRSEWPADLLPALAEAAGDPTLLAERNPLQYLGFA
                     LSDG"
     gene            2926..3372
                     /locus_tag="AC16JC_08975"
     CDS             2926..3372
                     /locus_tag="AC16JC_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HIR5164868.1"
                     /translation="MVDTTVVRLLVFRVGSLACAAEADTVREILPRLPTIRIPGAPLL
                     VAGLVNVRGSLVTVVDGWRALEQPAPSAADGGTTVLLEVGPERRPLGFAVDEVLDLLA
                     IPDDQLERRERLPGVDPRLVRAVGRRGGQVFVVLDTEALLAPILTS"
BASE COUNT          486 a         1180 c         1268 g          550 t
ORIGIN      
        1 gcgatcatcg agttcgtggt ggaggatctg tccacgtggt acgtgcggct caaccgcccc
       61 cgcttctggg cccccgaccg ggcggccgac gaagcggcgg tcgccacgct gcacgaggcg
      121 ctggtgacca cggcccggct gctcgcaccg gcggccccgt tcctcgcgga ctggctgcac
      181 cggtcgctgc tgggcacttc ggtacacctg gagcggtttc cccaggatcg ggggcggcgc
      241 gagccggaac tgctggcggc gatggcggcg gtgcgttgcc tcgcatccct ggcacgggcg
      301 gcccgggagt cgcgcagtct gcgggtgcgc cagcccgttg cccggatgca gctggccgtc
      361 ccggccgcgg tgcgggggcc gctcttggcg gacctgttag atatcttggt cgccgaggtg
      421 aacgtgaagt cgtaccaggt cgtggaatcc gacgacgagc tggtcacgtt gcacgggaaa
      481 gcgaacttcc gctccctcgg ccggcggtac ggcaaggcga cgccgcaggc ggccgccgcc
      541 gtcgcggggc tggcgccaca tgagttgcac gccctcgagc ggggcgaggc ggtgcgcagc
      601 ggggagtggg agttcctccc cgaggacgtg accgtcaccc gcgacgtcgc cagcgaatgg
      661 atcgtgcagg ccgacggccc ttacgtggtg gcgctcgatc cgtcgctgac gccggatctc
      721 gagcaggaag gcctggcgcg ggaggtcgtg aaccgcgtcc agcggctccg caaggaggcc
      781 gggtatgaat ataccacgcg catcgagctg agcgtctcgg gggccccgga ggtgatcgcg
      841 gccgtggagg cgtttgaggg gtacgtcgcg ggggaaacgc tggcccggcg catgcagttg
      901 gtcggcaccc tcgaggacgc ggatatcacc cgggaagtcg acattgaagg gcgggccgtg
      961 acgctcgcct tgcgacgtca cgacgcacga aagggcggaa cccgagaatg acgacgaaga
     1021 ccaaggggct gaacaagaaa cagctggcgc acctggaaaa gcggctgctg gaggagcgcg
     1081 cgcgcgtgat gaaggagctc ggccattacg gcgagtcctt caactcgagc ctgcaggcct
     1141 ccgacggcga cctgtcgagc tactccttcc acatggccga ccagggcacc gacgcgatgg
     1201 agcgtgagaa ggcgttcctg ttcgcctcgc aggaagggcg ctatctgtgg cacgtcaacc
     1261 aggccctgcg ccggctgtac cagacgccgg agaagttcgg caagtgcgag cagtgcgggg
     1321 aggagatcgg gttcgatcgc ctcgacgccc tgccgcacgc ccgtctctgc atcaactgca
     1381 aggcgaagga ggaggatggc aagcgccgct agccgccggc tgttctgggg cacgatcgtg
     1441 ggcatcgtcg ccctcgatct cctcaccaag tacctcgccg aaggcgtgct ggcgcgccgg
     1501 ccggtcccgg tgatcggcga ctacgtcgtg ctgcgcctgg tgtacaacca gtgtgccgcg
     1561 ttcggcctgt gtctcgggcc gcccgagtat tcgcgctgga tcttcctcgt gctggcactc
     1621 ctcgccctga cggtgctcgg ctcgatggtg cgccacacgc ccgatcacga ccggctgcgg
     1681 ctgttcgtgc tggccctcct cgtcgccggg gccatcggca acgtgctcga tcgcgtgcgc
     1741 agcgcccagg gcgtcgtcga cttcatcgaa gtgggcatcg ggagctaccg ctggccgacc
     1801 ttcaacgtcg ccgattcggc catcacggtc ggcgcgatcg cgctcgcggt gtcgctgtgg
     1861 ctcgagggac gcgcccgcgg cagcggggcg gcgaaggagg ccgggacggc cccctcgtga
     1921 cccccgacgg caccaccttc tgcatcgtgg tgccggtcac cgagcgcctc gaccgcttcc
     1981 tcgccgacca gctcgcgctc tcccgtaccc aggccgcccg tttgatcgcc gacaagcgcg
     2041 tgagcgccga cggcaagccg ttgcgcgcct ccgccctgct ggagcgcggc gccgtcgtga
     2101 ccgtggaatt cccgcccacc gcgccgcccc gcacctacgc gccggcccac accgacctcc
     2161 gcttcgtgtt cgaggacgag cacctcgccg tgctcgacaa acccgcgggc ctggtcgtcc
     2221 accccggccc cggccactgg gacgacacgc tggtcaacgc gctggtcggc cggggcacgg
     2281 ccctgtcgga agggtccacg gccgggcggc ccggcatcgt ccaccggctg gaccgcgaca
     2341 cgtcgggatt gctgctcgtg gccaagaccg atctggcgca tcgccggctg gcccgcgcca
     2401 tcgaacgccg ggaggtggag cgggtgtacg cgtgcgtcac ctgggggcat ttcgccaaga
     2461 gcccggtgga tatcgaggcc cccatcgccc gtcacatcga ggaccggaaa cggatgacgg
     2521 tgcgcgccgg agggcggcac gccgtcacgc acgtcgaagt cgtggcccgt tttccgctgg
     2581 tcgatctgct gcgcgtccgg ctcggcaccg ggcgcaccca ccagattcgc gtgcacgtgg
     2641 cccacgtcgg tcatccggtc gtcggagacc gggactacgc gttcggcggg agccggcgcg
     2701 tgactccctc agagcgccgt gccgcggaac ggctcgagag tctggcgccg cgccagctgc
     2761 tgcacgcagc gatgctgtcg ttccgccatc cggtgaccgg ggacgccctc acgctgcgct
     2821 ccgaatggcc cgcggacctg cttccggccc tggccgaggc ggcgggagat ccaacattgc
     2881 ttgctgaacg caaccccttg cagtatcttg gcttcgctct atcggatggt tgacacgacc
     2941 gttgttcgct tgctggtatt ccgggtgggg tcgttggcct gcgcggccga ggcggacacg
     3001 gtgcgggaga tcctcccccg gcttccgacc atccggatcc ccggggcgcc gctgctggtg
     3061 gcgggcctcg tgaacgtgcg gggcagcctg gtgacggtgg tggacgggtg gcgggccctc
     3121 gagcagccgg cgccctcggc ggcagacgga gggacgacgg tgctgctcga agtcgggccg
     3181 gagcggcgtc cgctcggttt cgccgtggat gaggtgctgg acctgctggc gattcccgac
     3241 gatcagctgg agcgccggga gcgtctgccg ggtgtcgatc cccgtctggt gcgcgcggtg
     3301 ggccgccgcg gcgggcaggt gttcgtcgtg ctggatacgg aagccttgct ggccccgata
     3361 ctgacttcct agggagggtc tgtgagccac tctgttctcg tctgcgacga cgcgatcttc
     3421 atgcggacga tgatcagcga catcctggcg caggccggct tcgaggtagt gggcgaggcg
     3481 gaga
//
LOCUS       DBNGZW010000381         7400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_787475, whole genome shotgun sequence.
ACCESSION   DBNGZW010000381 DBNGZW010000000
VERSION     DBNGZW010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_787475"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="AC16JC_08980"
     CDS             <1..917
                     /locus_tag="AC16JC_08980"
                     /inference="COORDINATES: protein motif:HMM:NF025736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR5164869.1"
                     /translation="VMEGRLVAARFTALDRWTLGYVTLATVALGLFSTAATIPRWPYL
                     AAAHAMLVALVLLAPYARESGPVGRFLGEWYPMLLLAGLYGEIGVLTLASGYQHDLAI
                     QRLETAVFGSQVSYRWIREMPVPWLSAVLHTCYLAYYAILFAPPLGLWFSGRREAARH
                     AILGVMITFYLCYAVFLFYPVAGPRYKFALPHNAATAVGPARLTQWLLDRGDSWGAAF
                     PSSHVAASVVATGFALRYWRPLGFALLLPTTGLVLAVVYGQFHYGVDALTGLLVAGVM
                     LAVMQRARVAEAPARAGESVVGVLDAST"
     gene            complement(885..1997)
                     /locus_tag="AC16JC_08985"
     CDS             complement(885..1997)
                     /locus_tag="AC16JC_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164870.1"
                     /translation="MPFFVFLPLPLLLLQAADTPGDWVRALPPAVIQVFPERHELVIE
                     LAPVDLPAGMHHDEMAGMDMPGMQMSEHSAHNPVFPPVMGVDLPLTGWMSGFRIELVD
                     SAGRSVPAQLLHHYNLIDPGHRELFLPISRRVLAAGSETGAVHMTRWLFGAPFVRGDR
                     LVASAMLHNPTMQSYRGVRTRLILTYTDDGHWYPLLRAYPWQFDVAFPVGDKSFDLPP
                     GRSEKFYEGSPSVPGKIVGIGGHVHDHAIALELRDVTTGDLLWRATPITDSTGHVVGM
                     PIKKFIGLTGVGTRIAPDHRYRVTVFYDNPTGVTLPEGGMGVVAGLFRPASGVTWPAA
                     DTADSLYVRDLRHALRLGEASLAVIRSGTGVEHAHH"
     gene            complement(2047..2505)
                     /locus_tag="AC16JC_08990"
     CDS             complement(2047..2505)
                     /locus_tag="AC16JC_08990"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR5164871.1"
                     /translation="MQFGHFGIALAIASLDPRPETFAVVGAAQFLPNLDSLVVRAGWA
                     RADFHGTWSHSLLCCLAVGAVATLLFGPWLGALALLSIVCHVVADMPTDTGIPLLLPF
                     SRRRFSFNLWQNTGYWGTSMYIGYYRHPWAWILEGAVFIVVLWRYGRLLA"
     gene            complement(2563..2805)
                     /locus_tag="AC16JC_08995"
     CDS             complement(2563..2805)
                     /locus_tag="AC16JC_08995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164872.1"
                     /translation="MTRRCPQCGRELSSFAVRCACGFELPEARDLRSDPDHPRCAACG
                     NALAIMTERCPSCGAVGYPALRGRRGKKSQGSPGNA"
     gene            complement(2802..3863)
                     /locus_tag="AC16JC_09000"
     CDS             complement(2802..3863)
                     /locus_tag="AC16JC_09000"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5164873.1"
                     /translation="MNVSNQMAMRMLIKGIHNRVRKLPLSLSFEVTHACTANCWHCNW
                     GGPIKETRRSAAEYAAICRDLQPVVVNVSGGEPMARGDLDDIVRALANPGRLPWVVVV
                     SNASQLTPDRFLRLKRAGMHQLSLSIDFPDDRHSDFRRIPGLFDHMAEVVPQCVALGD
                     PDDVSLNCCITAWNYRAVPDVVRLAARWGVSVNFSAYTPLRMDDPSGLVRHNGSAAEL
                     KEKIAEVVALKQEGYPVYTSARVLWKFHDFLVGGRAPGCQAGYRFLVVNPDGRLTPCA
                     MVMAYFDDHRSMQREFSRHNSCEACFISTRANCEKTTWESLADNADTLRQLLPWRWSR
                     RANGTRRSAPLPVLEESPQ"
     gene            complement(3945..4670)
                     /locus_tag="AC16JC_09005"
     CDS             complement(3945..4670)
                     /locus_tag="AC16JC_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SNARE-associated domain-containing protein"
                     /protein_id="HIR5164874.1"
                     /translation="MEMTLAVESIGPWRRLRRALVRPRGDRRWDVFLRATAVVALLGI
                     PVVLVFPDSVTYVWLAVLSLPANSPLSPILPTFFEPIIIEAAKWKPPWAVTLVATCGY
                     MYMEYINWHVYAWVLNWDRLAGFRARRSVRWGVGLFSRSPFWMVVIFAFTPLPFWAGR
                     ALAILHRYPLRRFLVATAVGRVPRWLFYAWFGGQVDVPTWVLAGAIVVPAAIVIGARL
                     LRGEALLAETAASPDDGEPPAVA"
     gene            complement(4729..6633)
                     /locus_tag="AC16JC_09010"
     CDS             complement(4729..6633)
                     /locus_tag="AC16JC_09010"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5164875.1"
                     /translation="MRSPPVVHARVLGPARVTVNGADAPAELLWRKHLALLIYLARSP
                     RGTRTREHLIGLLWSDRDEKSARHSLSEGLRVFRRVLGDARVRTDIDQVALEPGALTL
                     DCDELSARCAAADWAGAAALVQGEFLEGLAVPDANEFENWVATERIAWRARGVEALTR
                     WAEASVAAGAVEDARAATLRALALDPTAEPAARAAMRALALAGDRAAALDIGARVAAA
                     LRERLGAGLAPETQRLMDRVREARVGRRVAAAPSGTRPRPPLVGREDAQGRLGTAWRR
                     VAAGSGEVILIEGEPGEGKTRLADDLLDRVRLEDATVAACRAVPSDARTVWSGLAGLV
                     AGGLAVAPGLAGAPPAALAALGALDPEFGARVPAIGGEGGLEPSGAFAAAVRAAAEEL
                     PVLLAIDDAQHLDDATAHALPRLARDLREHRVLIVLCVAAGGSRVDWVEELRAHLGRE
                     LRGDVIRLERLDTGALATLARWWLPRYNEEAIARIARRLERDTAGVPLLATAVLEAVA
                     DGFRLSPDSPAWPSERRTLVDTLPGDLPPAVIGAVCLQFRQLPQGAQRVLGAAAALGE
                     RMDAPALCRATGLASGDVDQALDRLEWDRWLASDARGYAFAAPIMRAILIQEMVSPGQ
                     VRRYRAAADP"
     gene            6695..>7400
                     /locus_tag="AC16JC_09015"
     CDS             6695..>7400
                     /locus_tag="AC16JC_09015"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5164876.1"
                     /translation="MNAPSLEDVQRAVGDHYEVLDLAGVGGMGAVFRAQHRTLGHLVA
                     VTVLPPDVATSQMRQDRFKREASLAAQLSHPNIVPVYEFDTREGLSFLIMPFVRGRTL
                     ESALVERQQVPVNEILRLLREIGAALDAAHRRGVVHRDVKPANIMIEEDSGRLLLTDF
                     GVAQIRTVSGALTAPGSVIGTPDYMSPEQMIGSPDVDGRADLYSLGLIAFEALTGTRP
                     SLGADRATLARSLRGAR"
BASE COUNT         1172 a         2676 c         2528 g         1024 t
ORIGIN      
        1 ctgtgatgga ggggcgattg gtagcagcga ggtttacggc actggaccgc tggactctgg
       61 ggtacgtcac gctggcgact gtggccctgg gactcttcag cacggcggcg acgatccccc
      121 gctggcccta cctggcagcc gcccacgcca tgctggtcgc acttgtcctg ctcgccccgt
      181 acgcccgtga atccggaccg gtgggccgat ttctcggcga gtggtacccg atgctgttgc
      241 tggcggggct gtacggcgag atcggcgtcc tgaccctcgc gtccggatac cagcacgatc
      301 tggcgattca gcggctggag actgcggtgt tcggctccca ggtgtcctac cgctggatcc
      361 gcgagatgcc ggtgccgtgg ctctcggcgg tgctgcacac ctgctacctg gcgtactatg
      421 cgattctctt cgcgccgccg ctcgggctct ggttctccgg acggcgcgag gccgcccgcc
      481 acgccatcct cggcgtcatg atcaccttct acctgtgcta cgccgtcttc ctcttctatc
      541 cagtggccgg cccgcgctac aagttcgcgc tgccgcacaa tgccgcgacg gccgtgggtc
      601 ccgcccgcct cacgcaatgg ctgctcgacc gcggcgattc ctggggcgcg gcgtttccct
      661 cgtctcacgt cgccgcgtcg gtggtggcga ccgggttcgc cctgcgctac tggcgcccgc
      721 tcggattcgc gctgctgctt cccactaccg ggctggtgct cgcggtggtg tacgggcagt
      781 tccactacgg cgtggacgcg ctgacgggct tgctcgtcgc cggcgtgatg ctggcggtga
      841 tgcagcgggc gcgcgtggcc gaggcgccgg cccgcgcggg cgaatcagtg gtgggcgtgc
      901 tcgacgccag tacctgaccg gatcacggcg agcgacgcct cgccgagtcg cagggcgtgg
      961 cgcaggtccc gcacgtacag cgaatcagcg gtatcggcgg cgggccacgt cacgccggac
     1021 gccgggcgga acagtcccgc caccacgccc atgcccccct cggggagcgt cacgcccgtc
     1081 ggattgtcgt agaacacggt cacgcggtac cggtgatccg gcgcgatccg ggtgccgacc
     1141 ccggtcagcc cgatgaactt cttgatgggc atccccacga cgtgcccggt cgaatcggtg
     1201 atcggcgtcg cgcgccagag gaggtcgccc gtcgtcacgt cgcgcagctc gagcgcgatg
     1261 gcgtggtcgt gcacgtgacc gccgatcccg acgatcttgc cgggcacgct gggactccct
     1321 tcgtagaact tctccgagcg accgggcggc agatcgaacg atttgtctcc caccgggaac
     1381 gcaacgtcga actgccaggg atacgcgcgc agcaggggat accagtgccc gtcgtcggtg
     1441 taggtgagga tgagccgcgt ccgcacgccg cggtagctct gcatcgtcgg attgtggagc
     1501 atggcgctcg cgaccaggcg atcgcccctg acgaacgggg cgccgaacag ccaacgggtc
     1561 atgtgcaccg cgccggtctc actcccggcg gccagcacgc gacgcgagat cgggaggaac
     1621 agctcccgat ggcccgggtc gatcaggttg tagtggtgca gcagctgggc cggcacgctc
     1681 cggccggccg agtcgacgag ctcgatgcgg aaccccgaca tccagccggt cagcggcaga
     1741 tcgacgccca tcacgggcgg gaacaccggg ttgtgggcgc tgtgctcgct catctgcatg
     1801 ccgggcatgt ccatcccagc catctcgtcg tggtgcatgc ccgcggggag atccaccggg
     1861 gcgagctcga tcaccagttc gtggcgctcg gggaagacct ggatcacggc cggcggcagc
     1921 gcgcgcaccc aatcgccggg cgtgtcggcg gcctgcagca gcagcagggg cagcggcaag
     1981 aacacgaaga atggcatgga agtggagaga aaatatagcg gcgtccggca caccgacgcc
     2041 atccgctcag gcgagcagtc tcccgtagcg ccagagcacc acgatgaaga ccgcgccctc
     2101 gagaatccag gcccagggat ggcggtagta gccgatatac atcgacgtgc cccaataccc
     2161 ggtgttctgc cagaggttga acgagaaccg gcggcgactg aacggcagca acagcggaat
     2221 ccccgtgtcg gtcggcatat ccgccaccac atggcacacg atgctgagca acgcgagcgc
     2281 cccgagccag gggccgaaca agagggtggc gacggccccg accgccaggc agcacagcag
     2341 gctgtggctc caggtgccgt ggaagtcggc gcgtgcccag ccggcgcgca ccaccagcga
     2401 gtcgagattc ggcaggaact gcgcggcgcc gactacggcg aacgtctccg gtcgcgggtc
     2461 gagcgaggcg atcgccagcg cgatcccgaa gtggccgaac tgcatcgtgt cccccgtgtg
     2521 aaatggtccg ggacacgttc ggcacaccac gtcaagccgg tttcaagcgt tgccggggga
     2581 cccctgactc ttcttgcccc gccgcccgcg cagggcggga taacccacgg caccgcagga
     2641 cgggcaccgc tccgtcatga tggcgagcgc gttgccgcat gcggcgcagc ggggatggtc
     2701 ggggtcggag cgcaggtcgc gtgcctcggg gagctcgaat ccgcaggcac agcgtacggc
     2761 aaaggaggac aattcgcggc cacactgcgg gcagcgccgg gtcactgcgg gctctcctcg
     2821 agcaccggca gcggggcgga ccgccgcgtg ccgttggcgc gccggctcca ccgccagggc
     2881 aacagctggc gcagcgtgtc cgcgttgtcg gccagcgact cccacgtggt cttctcgcag
     2941 ttggcccgcg tgctgatgaa gcacgcctcg cagctgttgt ggcgcgagaa ctcgcgctgc
     3001 atgctgcggt gatcgtcgaa atacgccatt accatcgcac acggcgtgag ccggccgtcc
     3061 ggattgacga ccagaaagcg gtagcccgcc tggcacccgg gggcacgacc gcccacgagg
     3121 aaatcgtgaa atttccagag cacgcgcgcc gacgtgtaca ccgggtagcc ctcctgcttg
     3181 agggcgacca cctctgcgat cttctccttc agctccgccg ccgagccgtt gtgccgtacg
     3241 aggccgctgg gatcgtccat gcgcagcggc gtgtaggccg agaagttgac actcaccccc
     3301 caccgggcgg cgaggcggac gacgtcgggc accgcgcgat agttccacgc ggtgatacag
     3361 cagttcagcg agacatcgtc gggatccccc agcgccacgc actgggggac gacctcggcc
     3421 atgtggtcga acagtccggg aatgcgccgg aagtcgctgt ggcgatcgtc ggggaaatcg
     3481 atcgagaggg acagttggtg catgcccgcc cgcttgaggc gcaggaaccg atccggcgtc
     3541 agctgcgacg cgttgctcac caccaccacc cacggcagcc gtcccgggtt ggcgagcgcc
     3601 cgcacgatgt cgtccaggtc tccccgcgcc atcggctcgc cgcccgagac gttcacgacg
     3661 acgggctgga ggtcccggca gatcgcggcg tactccgccg cgctgcggcg cgtttccttg
     3721 atgggaccac cccagttgca gtgccagcaa ttggccgtac acgcgtgggt gacctcgaac
     3781 gacaacgaca gcggcagctt gcgaacccga ttgtgtatcc ccttgatgag catccgcatc
     3841 gccatctgat tggaaacgtt catgcctccc ccgcgagtgg atgcacgccc cgccgaaccg
     3901 ctgccccgcc ccgtcccgtg cccgaaggta cgtccaaccc ctgatcacgc aacggccggc
     3961 ggctccccgt catcgggaga agcggccgtc tccgcgagca acgcctctcc gcgcagcagg
     4021 cgagcgccga tcacgatcgc ggcgggcacg acgatcgcgc cggcgagaac ccacgtcggg
     4081 acgtccacct gtccgccgaa ccacgcgtag aacagccagc gcggcacgcg tccgaccgcc
     4141 gtcgccacca ggaagcgccg caggggatag cggtgcagga tcgccagcgc gcggccggcc
     4201 cagaacggca gcggcgtgaa ggcgaagatc accaccatcc agaaaggtga gcgcgagaac
     4261 aaccctacgc cccagcgcac cgaccggcga gccctgaacc cggccaaccg gtcccaattc
     4321 agcacccagg catacacgtg ccagttgatg tactccatgt acatgtagcc acacgttgcg
     4381 accagcgtga ccgcccaggg gggcttccac ttggcggctt cgatgatgat gggctcgaag
     4441 aacgtcggga ggatcgggga aagcgggctg ttggcgggca gggacagcac ggccagccag
     4501 acgtaagtga ccgagtccgg gaagacgagc acgaccggaa taccgagcag cgccacaacc
     4561 gcggtagccc ggaggaagac atcccagcgc cggtcgccgc gcggccgtac cagcgcccgc
     4621 cgcaatcgcc gccacggacc gatggactcc accgccaagg tcatctccac gtctctgccc
     4681 ctcccgaaaa tcccgggtgg atcatggcgg tctcccctca agcggatgtc aaggatcggc
     4741 cgcggcacgg tagcgacgga cctggcccgg ggacaccatc tcctgaatca ggatggcccg
     4801 catgatcggg gcagcgaagg cgtagccgcg cgcgtcggat gccagccagc gatcccactc
     4861 caggcgatcg agcgcctgat cgacgtcgcc gctggcgagc cccgtggcgc gacagagcgc
     4921 cggcgcatcc atgcgctccc ccagcgccgc cgcggcgccg agcacccgtt gcgcgccctg
     4981 gggcagctgc cggaactgca agcacaccgc accgatcacc gcggggggga ggtctccggg
     5041 cagggtgtcc accagggtgc gccgttcgct tggccacgcg ggggagtcgg gcgagagacg
     5101 aaacccgtcg gcgacggcct ccaggacggc cgtggcgagc agcgggacgc cggcggtatc
     5161 acgctcgagg cggcgcgcga tccgcgcgat cgcttcctcg ttgtagcgcg gcagccacca
     5221 ccgcgccagc gtcgcgagcg cgccggtgtc gagccgctcg aggcgaatga cgtcgccccg
     5281 aagctcgcgg ccgaggtgcg cccgcagctc ctcgacccaa tccacccgcg accctcctgc
     5341 cgcgacacac agcacgatga gcacccggtg ctccctgagg tcgcgggcga gccgcgggag
     5401 ggcgtgcgcc gtcgcgtcgt ccaggtgctg tgcgtcgtcg atggcgagca ggacgggcag
     5461 ctcctccgcc gcagcccgca ccgcggcggc gaaggcgccc gacggctcga gcccgccctc
     5521 cccgccgatc gccgggaccc gcgcgccgaa ctccgggtcc agcgcgccca gggcggcgag
     5581 cgcggcggga ggcgcaccgg cgagccctgg agcgaccgcg aggccgcccg ccaccagccc
     5641 cgcgagaccg ctccagacgg tacgcgcgtc ggaggggacg gcccgacagg cagcgacggt
     5701 cgcgtcctcg agtcgcactc gatcgaggag gtcgtccgcc agccgggtct tgccctcccc
     5761 gggctcgcct tcgatgagga tcacctcgcc cgaccccgcg gcgacgcgcc gccacgccgt
     5821 accgagccgg ccctgcgcat cctcccgccc taccagcggc ggccgaggtc gcgtcccgct
     5881 cggcgccgcc gccacgcgcc gacccacgcg cgcttcgcgc acccggtcca tcagccgctg
     5941 cgtttccggc gccaggccgg ccccgagccg ttcgcgcagg gcggcggcga cccgcgcgcc
     6001 gatgtccagc gccgccgcgc gatcaccggc gagagccagc gcccgcatcg cggcccgtgc
     6061 cgcaggctcc gcggtcgggt ctagggccaa cgcccgcaac gtcgccgcgc gcgcgtcctc
     6121 cacggctccg gcggcgaccg acgcttccgc ccagcgggtc agcgcctcga ccccgcgcgc
     6181 ccgccaggcg atgcgctcgg tggcgaccca gttttcgaac tcgttcgcat ccggtaccgc
     6241 cagcccctcg aggaactcgc cctggacgag cgccgcggcg cccgcccagt cggcggccgc
     6301 gcaacgcgcc gacagctcgt cgcaatcgag ggtcaacgcg ccgggctcga gcgccacttg
     6361 atcgatgtcc gtacggaccc gggcgtcgcc gagcacgcgc cggaacacgc gcaatccctc
     6421 gctcaacgag tgccgcgccg atttctcgtc gcggtcgctc cacaacagcc cgatgaggtg
     6481 ctcgcgcgtg cgggtgccgc gtgggctgcg cgcgaggtag atgagcaacg ccaggtgttt
     6541 gcgccacaac agctccgccg gcgcgtcggc accattgacg gtaacgcgcg cgggaccgag
     6601 cacgcgtgca tggacgaccg gaggcgaacg catacggctc ggattatcgg aggtcgtgcg
     6661 gccctgagca aggcgccgta tcttgccccg ggcgatgaat gccccctcac tcgaagacgt
     6721 gcagcgcgcc gtcggcgatc actacgaggt cctcgacctt gccggcgtcg ggggcatggg
     6781 agcggtgttc cgcgcgcagc accgcacgct cgggcacctc gtcgccgtga cggtgctccc
     6841 ccccgacgtc gccacgagcc agatgcggca ggaccgattc aagcgcgaag cctcgctggc
     6901 ggcccagttg tcccatccga atatcgtccc cgtgtacgag ttcgatacgc gcgaaggcct
     6961 gtcgttcctc atcatgccct tcgtgcgcgg caggacgctg gagagcgcac tcgttgaacg
     7021 ccagcaggtg ccggtcaacg agatcctgcg cttgctccgg gaaatcggcg ccgccctcga
     7081 tgccgcccac cggcgcggcg tggtccaccg tgacgtgaaa ccggccaaca tcatgatcga
     7141 ggaggattcc ggtcggttgc tcctgacgga cttcggggtg gcgcagatcc ggaccgtcag
     7201 cggcgcgctg acggcacccg ggtcggtcat cgggaccccc gattacatgt cgcccgagca
     7261 gatgatcggg tcgccggacg tggacgggcg cgccgatctg tactcgctcg ggctcatcgc
     7321 cttcgaggcg ctcaccggca cccgcccctc cctcggcgcc gaccgcgcga cgctggcgcg
     7381 ctcattacga ggggcccgcc
//
LOCUS       DBNGZW010000382         1798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_707726, whole genome shotgun sequence.
ACCESSION   DBNGZW010000382 DBNGZW010000000
VERSION     DBNGZW010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_707726"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="AC16JC_09020"
     CDS             <1..1205
                     /locus_tag="AC16JC_09020"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007585644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5164877.1"
                     /translation="IFGPVLVSMTFRTPAEAVALANNTPYGLAASVWTENINLALDVA
                     PKIKAGVVWINSTNLFDAASGFGGYRESGFGREGGREGMWEYLKPAWDEGRGTGDESA
                     SDTVVEAEGPRPSSPVPLPPIDRTPKLFIGGKQVRPDQGYSRPVRGADGRVLGEVGEG
                     NRKDVRDAVEAAHAAEGWGRGAGHLRAQILYYIAENLSARSDEFAGRIAAQTGAPAER
                     GRGEVDAAIARLFSYAAWADKYDGAVHHVPIRGVTLAMHEPIGVIGIAAPDTDPLLGF
                     VSLVAPAIAMGNTVVALPSPAHPLSATDFYAVLETSDLPDGVVNIVTGDKDALVRVLA
                     EHLDVDAVWYCGGPDGARAVEEASAGNMKRTWLPGARDWLDARQAEGREFLRHATQVK
                     NIWIPYGE"
     gene            1237..1647
                     /locus_tag="AC16JC_09025"
     CDS             1237..1647
                     /locus_tag="AC16JC_09025"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR5164878.1"
                     /translation="MEHRVNYSETDQMGFVYHANYFVWLDMARTEHLRRTGVTYKEME
                     QEGVFLAVTDVHVRYRQPARYDDLVRIRCWVRDLASRRAIFGYAVERAETGELLATAE
                     TALIALDRRHGLTRIPAHVMERLHATADPAPIRF"
     gene            1613..>1798
                     /locus_tag="AC16JC_09030"
     CDS             1613..>1798
                     /locus_tag="AC16JC_09030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164879.1"
                     /translation="MRPPILPPFASSVVAALLLAAGASPAVAQATTPAQDEATVGLLA
                     HVLALSDARRFDGALLRQ"
BASE COUNT          281 a          592 c          651 g          274 t
ORIGIN      
        1 agatcttcgg cccggtgctc gtgtccatga ccttccggac gccggccgag gcggtcgccc
       61 tcgccaacaa cacgccctac gggctggcgg cgagcgtttg gacggagaac atcaacctcg
      121 cgctcgatgt ggcgccgaag atcaaggcgg gcgtcgtgtg gatcaactcg acgaacctgt
      181 tcgacgcggc gtcggggttc ggcggatacc gggagtcggg gttcggacgc gagggcgggc
      241 gggaggggat gtgggagtac ctcaagcccg catgggacga gggacgaggg acgggggacg
      301 agtcggcatc ggatacggtg gtggaggcgg aggggcctcg tccctcgtcc cccgtcccgc
      361 ttcccccaat cgatcgaacg ccaaagttgt ttatcggcgg gaagcaagtc cgccccgacc
      421 agggctacag ccgtccggtg cgtggggcgg acggacgggt gctgggagaa gtgggggagg
      481 ggaatcgcaa ggacgtgcgc gacgcggtgg aggccgccca cgcggccgag ggctggggac
      541 gaggcgccgg acatctgcgg gcccagatcc tctactacat cgccgagaat ctctcggctc
      601 gttccgacga attcgccggg cgcatcgccg cgcagaccgg tgctccggcg gagcgcgggc
      661 gcggcgaggt ggacgccgcc atcgcgcggc tgttctccta cgccgcctgg gccgacaaat
      721 acgacggcgc agtgcaccac gtgccgatcc gcggggtgac gctggcgatg cacgagccga
      781 tcggcgtgat cggcatcgcg gcgccggaca cggatccgct gctcgggttc gtgtcgctcg
      841 tggcgccggc cattgccatg ggcaataccg tggtcgcgct gccgtcgccg gcgcacccgc
      901 tctctgcgac cgacttctac gccgtgctcg agacctcgga cctgcccgac ggcgtcgtga
      961 acatcgtgac cggcgacaag gacgcgctgg tgcgggtgct ggcggagcat ctagatgtgg
     1021 acgcggtctg gtactgcggc ggcccggacg gcgcacgggc ggtggaagag gcgtccgccg
     1081 gcaacatgaa gcgcacctgg ctgccgggcg cccgcgactg gctcgatgcg cgtcaggcgg
     1141 aagggcgcga gtttctccgc cacgcgacgc aggtgaagaa catctggatc ccctacggcg
     1201 agtgaccgtc tgaaccacgc cgagctgatc agcgccatcg agcatcgcgt caactactcc
     1261 gaaaccgacc agatggggtt tgtctaccac gccaactatt tcgtctggct ggatatggcc
     1321 cgcacggaac acctgcggcg cacgggggtg acctacaagg aaatggaaca agagggcgtc
     1381 ttcctggcgg tgaccgacgt acacgtgcgg taccgccagc ccgcccgcta cgacgacctc
     1441 gtgcgcattc gttgctgggt gcgggacctc gcatcgcgcc gggccatttt cggatacgct
     1501 gtggagcgcg ccgaaacggg cgagctcctg gccacggcgg agaccgcctt gattgccctc
     1561 gatcgccggc acggcctgac ccgcattccg gcacacgtga tggagcgcct gcatgcgacc
     1621 gccgatcctg cccccattcg cttctagcgt ggtcgcggcg ctgttgcttg cggctggcgc
     1681 ctcaccggcc gtggcgcagg ctacgacccc ggcgcaggac gaagccacgg tcggcctgct
     1741 ggcgcacgtg ctggcgctct ctgacgcccg ccgcttcgac ggcgccttgc tgcgacag
//
LOCUS       DBNGZW010000383        11266 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_853799, whole genome shotgun sequence.
ACCESSION   DBNGZW010000383 DBNGZW010000000
VERSION     DBNGZW010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 11266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11266
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_853799"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1281)
                     /locus_tag="AC16JC_09035"
     CDS             complement(<1..1281)
                     /locus_tag="AC16JC_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR5164880.1"
                     /translation="MLTSFSLLGHWMEMRSRFATGRAVEALLQLAPPTARRVREGTEE
                     EVPLDVVAVADLLAVRPGDTIPVDGVVTDGSSYVDESMLTGEPVPVAKGPGAEVVGGT
                     RNQQGAFRFRATKVGADTALARIVALVRDAQSSKAPAQRLADLAGKVLVIVALVAGIV
                     TFAAWLLLGNQGVVFALSAAVAAVVIACPDALALATPTAITVGVGRAARGGVLFRNAT
                     VLEAAAGVNTVVFDKTGTLTEGRPSVTDLHPVTGVSEQELLHLAAAADAQSEHPLATA
                     IVAAARQRGLTVDPTSDFEAIPGHGVKASVHGRIVLVGNRGLLERFSVPAGLNAEAER
                     LRAEAKTALYIAADGRMMGLLAVADSIRPTARAAVAALHGLGVRVMLLTGDSRPTAEA
                     VARAIGIDEVRAEVLPGDKAMEVQRLERAGRRVAM"
     gene            complement(1342..1698)
                     /locus_tag="AC16JC_09040"
     CDS             complement(1342..1698)
                     /locus_tag="AC16JC_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164881.1"
                     /translation="MGAHSHGHSHPIEARAEHRRATDAHAGHGAHMVADMLRRFVGSA
                     ILTVPIVLYSPLGTSLVHRELVPPFGVSSGVAGLILTSLVVWWGGWPFLSSAARSLRR
                     GELTMMTLIATGILVS"
     gene            complement(1763..5671)
                     /locus_tag="AC16JC_09045"
     CDS             complement(1763..5671)
                     /locus_tag="AC16JC_09045"
                     /inference="COORDINATES: protein motif:HMM:NF018905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5164882.1"
                     /translation="MNRGRSILALALIGTLATCVEHRTPTSSEPGVSNVQPAAALTSA
                     TIAQTLLASGHDPNNLKIYTTASIAPAANALVTVAVLTHQSSSAAPAPALTGGGMTAW
                     DLVATTTFNGATPLDRVSIFRAMSASPGSGPITITSSVTVSNCQWIVSQWDGVETGGT
                     NGAGAIVQTGATSGSAVNGLTVTLAGFADPADVGYGVFGIASATAAATPGTGFTRIDE
                     QPSGESTTGDLFAEWAVNDVTVDATWSSKTAGALGVEIKAGGSSGGGGGGVSTSLSTL
                     AAAPSTFTAGAGTSTITVTAKDGSGNPISGATVVLAATGTGNTLTQPSAATDANGVAT
                     GTLASTVAEAKTVSAKINGAAVTQTAIVTVTHGPVDAGQSTVAAAPASVVPGAPSTIT
                     VTAKDANGNPISGATVVLAATGMRTLTQPADTTSASGVATGTVSGMMEETITVSATVD
                     GTAITQTATVVVVAQAAAAISQTLLTSGNNAANQKIYTTASISPAPNALITVAVLMRR
                     SAGAVTPTVTGGGMPAWDLVGSADFDTQSSPTKRGLIFRAMSATPGSGAITIKFANSV
                     SNVQWIVSQWDGVETGGTNGSGAIVQTGTARSDGATSLAALLAPFASSNDVAYGVAGV
                     ARNGPTVTPANGFTEIAEVSSGENSALEAEWAANQQTVGASWGTSTKAGMLAVEIKAG
                     NAGSVIPVASVEVSPTSAVIAVGGTVQLSAATKDAGGEPLVGRVVTWASDAPGIAAVS
                     STGLVTGSAAGGPVTITATSEGKSGTSSITVTATAEPVASVEVLPSSANIVVGGTVQL
                     TATPKDANGQPLTGRTITWASNATGVATVSTAGLVTGVAAGAATVTATSEGQSGSSSI
                     TVSTGGTAALVGQWSAVLPAPIVQVHQHLLLDGRVLSFGGSSGIPQVFDPATGLFTAV
                     PISEIMFCSGHSWLPDGRLLVSGGGTGNGLGHPNSDIFDPATASWIAGPNMAYARWYP
                     TVTTLPDGEVVTMAGADENGNPVPIPEVFNGTSWRQLTGASLTLPNYPRNFVAPDGRI
                     FTAGPSAGSRWLDVSGAGSWTAGPTMNYGSRTYGSAVMYEAGKIMFVGGNSTPTNTAE
                     IIDLNQPSPQWTYTGSLTYARWNLNATVLPTGEVLVTGGVNGDRSNPDLKVNATELWN
                     PATGTWTLLAPSAPLLRGYHSTTVLLPDGRLIHSGGGAGGGTIDNLNYEVFSPPYLFK
                     GARPSVTGVTGTAGYGQTLTVQTPDGASITKVTFIRFGSVTHAFDEGQRLVSLSFSQT
                     GDGISVTLPASRNIAPPGPYLLFLVNGNGVPSVGKIMLMQ"
     gene            complement(5938..6010)
                     /locus_tag="AC16JC_09050"
     tRNA            complement(5938..6010)
                     /locus_tag="AC16JC_09050"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5975..5977),aa:Ala,seq:tgc)
     gene            complement(6222..8051)
                     /locus_tag="AC16JC_09055"
     CDS             complement(6222..8051)
                     /locus_tag="AC16JC_09055"
                     /inference="COORDINATES: protein motif:HMM:NF013613.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leishmanolysin-related zinc
                     metalloendopeptidase"
                     /protein_id="HIR5164883.1"
                     /translation="MLLRRTIPFALALGAFACKGTEPFVPVPRAIDVLPGTVSFGAIG
                     GARVLTAVVLDQRGDTIPSPSVTWSAGNGTVASVDAAGHVSALGVGTTKVFASVQGAS
                     APLKDSATVSVVQVPAQLVKVAGDQQVDTASGTLPTAVAVQVNDSFAHPVTGIAVAFA
                     VTQGGGSVAPTSASTNAQGLATVEWTLGPAAGANALTATVAGTGITGNPASFAATAVA
                     AGSLSSVAAFAGDGQTGLTGFAVNQPPAVVLRDGNGAPIAGKAVTFAVIQGGGSIQGG
                     STVTDANGVAQVGSWSVTLGANALTATVQNAGMVLGNPVTFTATGATAAYHIDVRFPV
                     ATTPARQAVFTGAASKWESLIFGDVPDVSVNIQANACISGQPAIHETVDDIIIFAILD
                     SIDGPGKILGQAGPCFVRSGSQLPLIGVMEFDTADVAGLEASGRFALVIEHEMGHVLG
                     YGTIWGNLLVDPVTNGNPNADPHFVGSQGLAAFDRAGGLSYSLSAKVPVEDCVGFPPN
                     TCGQGSVNAHWRESVFNNELMTGFIDSGTNPLSVVSTASMGDLGYLVNYAGSDAYTVP
                     AAAAAAAAPAAPGTKLNLGNDILRMPILVVDRTGRVVQTIAPR"
     gene            8136..8963
                     /locus_tag="AC16JC_09060"
     CDS             8136..8963
                     /locus_tag="AC16JC_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5164884.1"
                     /translation="MPVTITPRRNFTLCACLAGLAVLACHRAPDVRPLRLGTTTTVRQ
                     SGMLAVAESLWREPQLATVIGPSGLILHAAAQGDLDVVLTHAPALETKLLGGGAALER
                     CPFVTSRFVVGGPAADPAGVRGAPDAAEAFRRIAAHAMTFVSRGDSSGTNEREVAVWR
                     SAGLEPWAGDHPWYLESGTDQTTTLRLADERRAYALADLPTLATLRDLTLQPLFGDDT
                     VLLNRYSITLVRTPVPHPEARRFFAWALDRWRAAVLAIRLRDGTAAFERSPGACVAS"
     gene            complement(9043..9786)
                     /locus_tag="AC16JC_09065"
     CDS             complement(9043..9786)
                     /locus_tag="AC16JC_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164885.1"
                     /translation="MKCNWQLRPVLAGASTLLVAGLIACEQPTDPASMPVPAFGFGNG
                     APTGAHYNLNWIGVPQGKTADMTGANGHVIFVSLQGRTNILLCESGVGADCADVTGFQ
                     VLDANGTDGSASFALPNPDPTNSGATVYSVYVRALGSPGGSATNTTCGVDPLSPTDTI
                     CSVVQLILNRQKGKSTFTNVTKELLYVYADVNGDGTIDRVPLFDSRLQDYFWQYDNTG
                     LKLAQFRFYPCSTTVPLDPGSSITTQCGP"
     gene            complement(10111..10518)
                     /locus_tag="AC16JC_09070"
     CDS             complement(10111..10518)
                     /locus_tag="AC16JC_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164886.1"
                     /translation="MITLRRPLAAACVALLAACGGDGTGPVAGSLKVNLTTPNNGHDV
                     AVMLLLTAPAPPAGVAVGGSGLTLWGTPITSTTAKVVVTGTLATGTILTLQVDDMNKL
                     SQYSVTLLSVAADSIGGFAVRKALSGYSASITR"
     gene            complement(10515..>11266)
                     /locus_tag="AC16JC_09075"
     CDS             complement(10515..>11266)
                     /locus_tag="AC16JC_09075"
                     /inference="COORDINATES: protein motif:HMM:NF017183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HIR5164887.1"
                     /translation="TGALAGATYTATLAREHRLDVTVGANGTVAYSPAADSSGTFIAQ
                     GTPVTLTATPIAPLVFGGWTGDTTAAAALLVLPMGRPFALSAQFDPQLAITSTSPRPN
                     ATMGAGYADTLRASGGGATQSWQVVLGSLPPGLVLSVTGRITGFPSQVGQFPFTVRVV
                     SGAQSQQQSYTITVTAPTLAAATVLAQLLNGTGALTIDELRYLDMLGNRTCGDPPTPS
                     CFDLGDFAAWVRTTGAQPALLFSAARKGGRP"
BASE COUNT         1663 a         4056 c         3849 g         1698 t
ORIGIN      
        1 catcgcgacg cggcggcccg cccgctccaa tcgctgcacc tccatggcct tgtcacccgg
       61 gagcacctcg gcgcgtacct cgtcgatgcc gatggcgcgc gccaccgctt cggccgtggg
      121 acggctgtcc cccgtcagca gcatcacccg cacgcccaga ccatgcagcg cggcgaccgc
      181 cgctcgtgcc gtcgggcgga tcgagtcggc gaccgcgagc agccccatca tgcgtccatc
      241 cgcggcgatg tacaacgcgg tcttcgcttc cgcccgcagg cgctcggcct cggcattcag
      301 cccggccggc accgagaacc gctcgagcag gccgcggttt cccacgagca cgatgcgtcc
      361 gtggaccgat gccttcacgc catgaccggg aatcgcctcg aagtccgatg tcggatcgac
      421 ggtgagtccg cgctgccgcg ccgccgcgac gatcgccgtg gccagcggat gctccgactg
      481 cgcgtccgcc gccgcggcca ggtgcagcag ctcctgctcc gatactcccg tcacgggatg
      541 caggtcggtc accgagggac gcccctccgt gagcgtgccc gtcttgtcga acacgaccgt
      601 attcacgccc gcggccgcct cgagcacggt cgcgttccgg aacaagacgc cgccgcgcgc
      661 cgcccggccc acgcccacgg tgatcgccgt cggggtcgcg agggcgagcg catcgggaca
      721 cgcgatcacc accgccgcca ccgccgccga gagggcgaag acgacgccct gatttcccaa
      781 caggagccag gcggcgaacg tcacgatccc ggccaccagc gccacgatca cgaggacctt
      841 gccggccagg tccgcgagcc gctgcgccgg cgctttggag gactgagcgt cgcgcaccag
      901 ggcgacgatg cgggccagcg cggtgtcggc acccaccttg gtcgcgcgaa atcgaaaggc
      961 gccctgctgg ttgcgtgtcc ctccgaccac ctccgcgccg ggtcccttcg ccacgggcac
     1021 gggctccccg gtcagcatcg attcgtccac gtagctcgag ccgtcggtca cgacgccgtc
     1081 gaccgggatg gtgtcgcccg gccggacggc gaggaggtcg gcgactgcga cgacgtcgag
     1141 cgggacttcc tcctcggtcc cctcccgcac gcggcgcgcc gtcggcggcg cgagctgcag
     1201 cagggcttcg accgcgcggc cggtggcgaa gcggctgcgc atctccatcc agtgtcccag
     1261 caggctgaag gacgtgagca tcgcggcggc atcgtagaaa tgctcgcccg gcagcccgaa
     1321 ggtgaccgcg accgagtacc cttagctcac gagaatcccg gtcgcgatca acgtcatcat
     1381 cgtcagctcg ccgcgccgca gcgaacgggc cgccgacgac aggaacggcc acccgcccca
     1441 ccagacgacg agcgacgtca ggatgagccc tgcgacgccc gacgacacgc caaagggcgg
     1501 gaccagctcg cggtgaacga gcgacgtgcc gagcggcgag tacaggacga tcggaacggt
     1561 gaggatcgcc gatccgacga agcgtcgcag catgtccgcg accatgtgcg cgccgtggcc
     1621 ggcatgcgca tcggtcgcgc ggcggtgctc ggcccgggct tcgatcgggt gggaatgacc
     1681 atgggaatga gcgcccatgg aaggaagaaa gcccggcccc ccgggtgttc gccagggggc
     1741 cggactcttc agtctcctaa gcttactgca ttagcatgat cttccccacc gacggcacgc
     1801 cattgccgtt caccaggaac agcagatacg gccccggcgg tgcgatgttg cggctggccg
     1861 gcagcgtcac cgaaatgccg tcgcccgtct gcgagaagct cagcgacacc agccgctggc
     1921 cctcgtcgaa ggcgtgcgtc accgagccga accggatgaa ggtcaccttc gtgatgctgg
     1981 cgccgtccgg cgtctgtacc gtcaacgtct ggccgtaccc cgccgtcccg gtcacgccgg
     2041 tgacgctcgg ccgcgcaccc ttgaagagat acggcggcga gaagacttcg tagttgaggt
     2101 tgtcgatcgt cccgccaccc gcgccgccgc cactgtgaat cagccgtccg tccggcagca
     2161 acaccgtggt cgagtggtac ccgcggagca acggagcgct cggcgccagc agcgtccacg
     2221 tgccggtcgc gggattccag agctccgtcg cgttgacctt caggtcggga ttcgagcgat
     2281 cgccgttcac gccgccggtg acgagcacct cgcccgtcgg caggacggtc gcgttgaggt
     2341 tccagcgggc gtaggtcagc gagccggtat acgtccactg cggactgggc tgattcaggt
     2401 cgatgatctc ggcggtgttc gtcggcgtcg agttgccgcc cacgaacatg atcttccccg
     2461 cctcgtacat caccgcggag ccgtaggtgc gcgacccgta gttcatcgtc ggccccgcgg
     2521 tccagctgcc ggcgccggag acgtcgagcc agcgggatcc cgccgacgga ccggcggtga
     2581 agatgcgccc gtcgggcgcc acgaagttcc gggggtaatt cggcagcgtg agactcgccc
     2641 cggtgagctg gcgccagctc gtcccgttga agacctccgg gatcggcacg ggattcccgt
     2701 tctcgtccgc gcccgccatc gtcaccactt cgccgtccgg cagcgtggtc acggtcgggt
     2761 accaccgggc atacgccatg ttcggtccgg cgatccacga agccgtggcg ggatcgaaga
     2821 tgtcggagtt ggggtggccg agaccgtttc cggtgccccc gccactcacc agcaggcgcc
     2881 catccgggag ccagctgtgg ccgctgcaga acatgatctc cgaaatgggc acggccgtga
     2941 agaggccggt cgcgggatcg aacacctgcg gaatgccgct gctgcccccg aagctcagca
     3001 cccggccgtc gagcaacagg tgctggtgca cctggacgat cggggccggc agcaccgccg
     3061 accactggcc caccagcgcc gcggtcccac ccgtcgacac cgtgatgctc gagctcccgc
     3121 tctgcccctc gctcgtcgcc gtgaccgtcg ccgcccccgc ggccaccccg gtcacgaggc
     3181 cggcggtcga caccgtcgcc accccggtcg cgttgctcgc ccacgtgatc gtgcgtccgg
     3241 tcagcggctg gccgttggcg tccttcggag tggccgtgag ctgcacggtg ccccccacca
     3301 cgatattggc cgaactcggc agcacctcga ccgacgccac cggctccgcc gtcgcggtca
     3361 ccgtgatgct cgacgtcccg gacttccctt cactggttgc ggtgatcgtc acgggtccac
     3421 cggcggcgct gccggtgacg aggccggtgg acgagacagc ggcgatcccg ggagcatcgg
     3481 acgcccaggt gaccacgcga ccgaccagcg gctcgccacc ggcgtccttg gtggcggcgc
     3541 tgagctgcac cgtgcccccg accgcaatga ccgccgacgt cggactgacc tcgaccgacg
     3601 ccaccggaat cacggaaccg gcgttccccg ccttgatctc gaccgccagc attcccgcct
     3661 tggtggacgt cccccagctc gccccgaccg tctgctgatt ggccgcccac tcggcctcca
     3721 gcgccgagtt ctctccggac gacacctcgg cgatctcagt gaacccgttg gcgggcgtca
     3781 cggtcggccc gttcctcgcc acgccggcga cgccgtacgc gacgtcgttg ctggaggcga
     3841 acggagcgag cagggccgcg aggctcgtcg ccccgtcgct gcgcgccgtg ccggtctgga
     3901 cgatcgcgcc ggacccgtta gtgccgccgg tctcgacgcc gtcccactgc gacacgatcc
     3961 actgcacgtt ggacacgctg ttcgcgaact tgatcgtgat ggcgccgctg cccggtgtgg
     4021 cgctcatcgc ccggaagatc aagccgcgct tggtgggcga gctctgggta tcgaagtccg
     4081 cgcttcccac caggtcccac gcgggcatgc cgccgcccgt gacggtggga gtgaccgccc
     4141 cggcggagcg gcgcatcagc acggccaccg tgatgagcgc attgggcgcg ggcgagatcg
     4201 acgcggtcgt gtagatcttc tgattcgccg cgttgttgcc ggacgtaagc agcgtctggc
     4261 ttatcgccgc ggcggcttgc gccacgacta ccacggtggc ggtctgcgta atcgccgtgc
     4321 cgtcgaccgt ggccgagacg gtgatcgtct cctccatcat cccgctgacc gtgccggtcg
     4381 ccacgccgct cgcgctggtc gtgtccgcgg gttgcgtgag agtccgcatc cccgtcgccg
     4441 ccagcacgac tgtggccccg ctgatcgggt tgccgttggc atccttcgct gtcaccgtga
     4501 tcgtggacgg tgcgccggga accaccgagg ccggtgcagc cgcgacggtc gactggcccg
     4561 cgtccaccgg cccatgcgtc acggtgacga tcgcggtttg cgtgaccgcc gccccgttga
     4621 tcttggccga gacggtcttg gcctccgcca cagtcgaggc gagggtcccc gtcgccacgc
     4681 cgttggcgtc ggtcgcggcc gaaggctgcg tcagggtgtt gccggtgccg gtggccgcga
     4741 ggaccaccgt ggcgccgctg atcgggttgc cgctgccatc cttcgcggtc accgtgatcg
     4801 tggaggtgcc ggcgccggcg gtgaacgtgc tcggggcagc cgccagggtg gacagggagg
     4861 tcgacacgcc gcctccaccg cccccgctgc tgccgcccgc cttgatttcg acgcccagtg
     4921 ccccggcggt cttgctcgac caggtcgcgt cgaccgtgac gtcgttcacg gcccattccg
     4981 cgaacagatc gcccgtcgtg ctttcacccg acggttgctc gtcgatccgc gtgaagcccg
     5041 tgccaggcgt ggcggctgcg gtcgcgctgg cgatgccgaa cacgccgtag cccacgtcgg
     5101 cggggtccgc gaagccggcc agcgtcaccg tcaggccgtt cacggccgag ccgctggtcg
     5161 cgcccgtctg cacgatcgcc ccggcgccgt tcgtgccgcc ggtctcgacc ccatcccact
     5221 gcgacacgat ccactggcag ttggacaccg tgacgctcga cgtgatggtg atcggcccgc
     5281 tgccggggga ggcgctcatg gcgcggaaga tcgagacgcg atcgagcggc gtggccccgt
     5341 tgaaggtggt cgtggccacc aggtcccagg cggtcatgcc gccgcccgtg agcgcgggag
     5401 ccggagcggc cgaggaggat tggtgggtca gcaccgcgac cgtgacgagc gcattggccg
     5461 ccggggcgat cgacgccgtc gtgtagatct tgaggttgtt ggggtcgtgt cccgaggcga
     5521 gcagcgtctg ggcgatcgtc gcggaagtca atgcagccgc aggctggaca ttcgacactc
     5581 ccggctccga ggaggtcggc gtccgatgct cgacacacgt tgcgagcgtg ccgatcagcg
     5641 ccaaagccag gatgctgcgg ccacgattca tgggacctcc gggacgttgt cgtccctgcc
     5701 cccagcgtag tgggggagca gggaccagga tctgacagcg gatcgccacc gtcgctggcg
     5761 tgcccggaaa gcaactgcgt ggccgtcaat ttcgcgtcaa ttcaccgacc tgcggcacgg
     5821 gcgccacaca acacggtgaa cgccgttaag gggcaggaat cgtgcgcaag ggaagggccg
     5881 cctcaggggc tggacccggc tcagggaggt gtggcgccgc agcaacgacc tgcggaatgg
     5941 agcggatggg attcgaaccc acgaccccct ggttgcaaac caggtgctct cccagctgag
     6001 ctaccgcccc ccggttacaa attagcaggg aacgaggagt gggggcgaga cggcgaggac
     6061 gccgggaacg cgcagccgcc cagggcgtct gagccgttag ggaaactctg cacaagtccg
     6121 tgcgacgccg ggggggagcg actatgtccg ggccgtgcgg cccccgcgtc ctcgcgtcct
     6181 ctcccccacc cccggcgtcg ccccacttat gcagagcttc cctagcgcgg cgcgatcgtc
     6241 tgcaccaccc tccccgtccg gtccaccacc aggatcggca tgcggaggat gtcgttcccc
     6301 aggttcagct tcgtcccggg cgcggcaggt gccgcagcgg cggcggccgc cgccgggacg
     6361 gtgtaggcgt ctgatcccgc gtagttcacc aggtagccca ggtcgcccat cgatgccgtg
     6421 gagacgaccg acagcgggtt ggtcccggag tcgatgaacc cggtcatcag ctcgttattg
     6481 aagacggact cccgccaatg cgcgttcacg gatccctgtc cgcaggtgtt gggtgggaag
     6541 cccacacaat cctcgaccgg gactttggcg ctcaggctgt agctcaaccc gcccgcgcga
     6601 tcgaacgcgg cgagcccctg gctccccacg aagtgagggt ccgcattggg attgccgttc
     6661 gtcacgggat cgacgagcaa gttcccccag atcgtcccgt agcccagcac gtgccccatc
     6721 tcgtgctcga tcacgagcgc aaagcggccc gaagcctcca gtcccgccac gtccgccgtg
     6781 tcgaactcca tcacgccgat caatggcaac tggctccccg accgcacgaa gcatggcccg
     6841 gcctgtccca ggatcttgcc cggaccgtcg atcgagtcga gtatcgcgaa gatgatgatg
     6901 tcgtccaccg tctcgtggat cgcgggttgg ccgctgatgc atgcgttcgc ctggatgttg
     6961 accgacacgt cgggcacatc gccgaagatc agcgactccc acttgctcgc cgcaccggta
     7021 aacaccgcct gacgcgcggg cgtcgtcgcc accgggaacc gtacgtcgat gtgatacgcg
     7081 gcggtcgcgc cggttgccgt gaacgtcacc gggttgccga gcaccatgcc tgcgttctgg
     7141 accgtggccg tgagcgcgtt ggcgccgagg gtcacactcc agctgcccac ctgggcgacg
     7201 ccgttcgcat cggtcaccgt ggatccgccc tggatgctcc cgccaccctg gatgacggcg
     7261 aacgtcacgg ccttccccgc gatcggcgcg ccgttcccgt cacgcagcac caccgcgggc
     7321 ggttggttca ccgcgaaccc cgtgagcccc gtctgcccgt cgcccgcgaa cgccgccacg
     7381 ctcgacagtg agcccgcggc gaccgccgtg gccgcgaagc tcgcgggatt cccggtgatc
     7441 ccagtgcccg ccaccgtcgc cgtgagggca ttcgcaccgg ccgcgggccc gagcgtccac
     7501 tcgaccgtcg ccagcccctg tgcattggtc gaagcgctcg tcggtgcgac gctgccgccg
     7561 ccctgcgtca cggcgaaggc cacggcgatc cccgtgaccg gatgggcgaa cgaatcgttg
     7621 acctgcaccg cgaccgccgt gggcagcgtg ccggacgccg tgtccacctg ttgatcgccc
     7681 gccaccttca cgagctgcgc cggcacctgg acgaccgaca cggtagcgct gtccttgagc
     7741 ggcgccgagg cgccctgcac gctcgcgaag accttcgtcg tccccacgcc gagcgcactc
     7801 acatgccccg ccgcgtcgac gctggccacc gtgccgttgc cggcgctcca cgtcacgctc
     7861 gggctcggga tcgtgtcgcc gcgctgatcc agcaccaccg ccgtgagcac ccgcgcgccc
     7921 ccgatggccc cgaagctcac cgtgccgggc agcacgtcga tcgccctcgg gaccgggacg
     7981 aacggctcgg tgcccttgca ggcgaatgca ccgagggcca gcgcgaaggg aatcgtgcga
     8041 cgcaacagca tgggcttctc gcaaaaggga tctcgatgca acgtagagca tcgcgccacc
     8101 gccaacaagt cacggcgcgg tcaggaggtc gtgtgatgcc agtcacaatc acgccgcgcc
     8161 gcaatttcac gctctgcgcc tgcctggcgg ggctcgccgt cctcgcctgc caccgggctc
     8221 cggacgtccg gccgctgcgc ctcggcacca ccacgaccgt gcggcaatcc ggcatgctgg
     8281 ccgtggcgga atcgctgtgg cgggagccgc agctcgcgac cgtcatcggc ccgtcaggcc
     8341 tgatcctcca cgccgccgcc cagggagacc tggacgtcgt cctgacacac gcccccgccc
     8401 tggagacgaa gctccttggc ggcggcgctg ccctggagcg ctgccccttc gtcaccagcc
     8461 ggttcgtcgt gggggggccc gctgccgacc cggccggcgt gcgcggcgcc ccggacgcgg
     8521 ccgaggcgtt tcgccgcatc gcggcgcatg ccatgacgtt cgtttcgcgc ggcgattcct
     8581 cagggacgaa cgagcgggag gtggcggtgt ggcgctcggc ggggcttgag ccgtgggccg
     8641 gcgaccatcc ctggtatctc gaatcgggca ccgaccagac aacgacgctg cgactcgccg
     8701 acgagcgccg ggcctacgcc ctggccgatc tcccgacgct cgccacgctg cgcgacctga
     8761 cgctgcagcc gctgttcggc gacgacaccg tactgctcaa ccgctactcg atcaccctcg
     8821 tccggacgcc agtgccccat cccgaagcgc ggcgcttctt cgcgtgggcg ctcgaccggt
     8881 ggcgcgccgc ggtgctcgcc atccggctgc gcgacggcac ggccgcgttc gagcggtcgc
     8941 ccggcgcttg cgttgcctcg tgatccgctc ggagcgagcc gcccgctggg gcacagcctg
     9001 agctgtgcct cagcgaacgg ctcggtggac ttcagccctt cctcagggcc cgcattgagt
     9061 ggtgatgctg ctgccgggat cgagcggcac ggtggtgctg cagggataga agcggaactg
     9121 tgcgagcttg agcccggtgt tgtcgtactg ccagaagtag tcctggagcc gggagtcgaa
     9181 cagcggcacg cggtcgatgg tgccgtcccc gttcacgtcc gcgtacacgt acagcagctc
     9241 cttcgtgacg ttcgtgaacg tggacttccc cttctggcgg ttgaggatca gctgcacgac
     9301 cgagcagatg gtatcggtcg gtgagagggg gtccacgccg caggtcgtgt tcgtggcgct
     9361 gccgcccggg ctgccaaggg cgcgcacgta gaccgagtac acggtggccc cgctattggt
     9421 gggatccggg ttcggcaggg cgaacgatgc cgagccgtcc gtgccgttgg cgtcgagcac
     9481 ctgaaagccg gtcacgtccg cgcagtccgc gccgaccccc gactcgcaga gcaggatgtt
     9541 ggtccgaccc tgcagcgaca cgaagatcac gtgtccgttg gcgccggtca tgtcggcggt
     9601 cttgccctgc ggcacgccga tccagttgag gttgtaatgc gcgccggtcg gggcgccgtt
     9661 gccgaagccg aacgcgggca ccggcatcga ggcagggtcg gtgggctgct cgcacgcgat
     9721 gagcccggcg acgagcaagg tgctggcgcc ggccaacacg ggccggagtt gccaattgca
     9781 cttcatggtt acctcccgag aacagagaag gagttgtcgg ccgatgcgct cgggatcggg
     9841 cggcaagagt ccgctgccac ctgcatcaca cacatggccg gatggcgaga ttggcgccgc
     9901 gggcggagga gggaggggag tcggacccgc ggcggcgacc tgcggtgcat gttgcatcgc
     9961 tccgcagact gctgcggagc ggccccgcgc gcgcaatctg gccactgccg gagggacctc
    10021 gcggtgacca gcggcccgat gaaacgggtc acatgccggc ccctgccgac atgtgacccg
    10081 agcggagggt caggtcggtc ggagccgggt ctacctcgtg atactggccg aataacccga
    10141 cagtgctttc cgcacggcga atccaccgat cgaatcggcc gccacggaca ggagcgtcac
    10201 gctgtactga ctcagcttgt tcatgtcgtc cacctgaagc gtcagaatcg tcccggtggc
    10261 cagggtcccc gtgacgacca ccttcgccgt ggtggacgtg atcggcgtgc cccacagggt
    10321 gaggcccgag ccccccaccg ctacgccggc gggcggcgcc ggcgcggtga gcagcagcat
    10381 cacggcgacg tcgtgcccgt tattgggcgt ggtgaggttc accttcagcg aacccgcgac
    10441 gggtccggtg ccgtcgccac cgcaggcggc cagaagcgcc acgcaggcgg cggcgagcgg
    10501 gcggcgcagc gtgatcatgg ccgccctcct ttccgcgcgg cgctgaacag cagcgcgggt
    10561 tgggcacccg tcgtccttac ccaggcggcg aagtctccca ggtcgaagca actcggcgtc
    10621 ggcggatccc cgcaggttcg gttgccgagc atgtcgagat agcgcagctc gtcgatggtg
    10681 agggcgcccg tcccgttgag cagctgcgcc agcacggtcg cggccgcgag cgtcggggcg
    10741 gtcacggtga tggtgtagct ctgctgctga ctctgcgcgc cggagaccac ccgcacggtg
    10801 aacgggaact gcccgacctg ggagggaaag cccgtgatcc ggcccgtcac cgagagcacg
    10861 agaccgggcg gcaggctgcc cagcacgacc tgccagctct gcgtcgcccc gcctccgctg
    10921 gcgcgcagcg tgtccgcgta ccccgccccc atggtcgcgt tcggccgcgg gctcgtgctc
    10981 gtgatggcga gctgcggatc gaactgggcg ctcagcgcaa agggccggcc cattgggagc
    11041 acgagcagcg cggcggcggc cgtcgtatca cccgtccagc cgccgaacac gagcggcgca
    11101 atgggcgtgg ccgtgagcgt caccggtgtg ccctgggcga tgaacgtccc gctcgaatcc
    11161 gcggcggggc tgtacgccac cgtgccgttc gcccccacgg tgacgtccag ccggtgctcg
    11221 cgcgcgagcg tcgccgtgta ggtggcgccg gccaaggcgc cggtga
//
LOCUS       DBNGZW010000384         3654 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1196864, whole genome shotgun sequence.
ACCESSION   DBNGZW010000384 DBNGZW010000000
VERSION     DBNGZW010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3654
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1196864"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..1266)
                     /gene="purD"
                     /locus_tag="AC16JC_09080"
     CDS             complement(43..1266)
                     /gene="purD"
                     /locus_tag="AC16JC_09080"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009113 - purine nucleobase biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HIR5164888.1"
                     /translation="MRVLVVGGGGREHALCWALHADDPSTTLFAAPGNPGTAALATNL
                     AVAATDLDGVVGAVRDHRIDLTVIGPEVPLAAGLVDRLRATGHPAFGPSAAAARIESS
                     KSFAKDVMASAGVPTAASRTFTEIAPALAHIEGHAEPLVVKASGLAAGKGAVVCATRS
                     DAAAVARDMLESGVLGEAGREIVVETFLEGEELSVLALTDGERLLILPGAQDHKRLGE
                     GDTGPNTGGMGAYCPVSLATPAMLERVRREVLVPTLAELVRRGCRYSGVLYAGLMIGT
                     DGTPWVLEFNCRFGDPETQVLLPVLPRGSTAHLAAIAADVWRPESTVLTANRAAVTTV
                     LAARGYPDRPERGAVIDIPALPPDVLLFHAGTTRDAGGALRVAGGRVLNVTALAPTVA
                     AAAAAEVPISRLRRR"
     gene            complement(1263..2267)
                     /locus_tag="AC16JC_09085"
     CDS             complement(1263..2267)
                     /locus_tag="AC16JC_09085"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR5164889.1"
                     /translation="MTDATSGGLPAARPRKILTQIAPVAWEHPADRAALQSLRSVPGF
                     DEVLKKIYGFLGERGVRLLFQADAVRVGPTQFPRLNQLYTDVLTTMDWSVRPELFVSQ
                     TPFANAGAFGMDKPFIVINSGALTLLDDDEMRSLLGHELGHVMSGHALYHTLLVLLLN
                     VSLSALPFLAGIAILPIQLALLEWFRKSELSSDRAGLLATQDPVASLRMNLKFAGGGD
                     MKQMDLNAFLVQAKEYEETGGAMDRVFKILGVLLRTHPFNTVRAAELQRWVEAGEYDR
                     ILRGEYPRRGPDVEQRPLEKDLDDAREHYMKEAKAVVNDVVDSAKRAAQAFSDAMKKK
                     "
     gene            complement(2323..3492)
                     /locus_tag="AC16JC_09090"
     CDS             complement(2323..3492)
                     /locus_tag="AC16JC_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR5164890.1"
                     /translation="MTKAFDADLRRRMGTRAVHGGMVPDPLSGAVMTPIYQTSTYVQE
                     GLGRHKGYEYGRTRNPTREALERSVAALEGGRHGLAYGSGLAAVDAVMKLLSAGDHVV
                     SGENVYGGTHRQMTLIWSRLGLSFTFVDGGNADAVAAAVTPRTKLIYAETPTNPMMRL
                     CDLAATAEIAKGAGALLVVDNTFATPCFQRPLEFGADLVLHSTTKYLNGHSDMVGGML
                     VTPRDDLAERLAFIQNGSGAVPGPFDCWLALRGIKTFPLRMRQHDANGRRVAEWLSHN
                     PRVRQVYYPGLPSHPQHDLACRQMSGFGGMLSVELGDPAFARRVVERTRVFALAESLG
                     GVESLIGHPASMTHASVPPELRQKMGLTDSLIRLSVGIEDIEDLIEDLDQALAAG"
BASE COUNT          541 a         1267 c         1314 g          532 t
ORIGIN      
        1 gctggacgcc gagcccatcg acatccggcc cacgaacacc ggttaccgcc gccgcaacct
       61 gctgatcggc acctccgccg ccgcggccgc cgcgacggtc ggcgccaggg ccgtgacgtt
      121 gagcacccgg ccccccgcga cgcgcagcgc gccgcccgca tcacgggtgg ttcccgcgtg
      181 gaacagcagc acgtcgggcg ggagggcggg aatgtcgatg acggcgcccc tctccggacg
      241 gtcgggatag ccgcgcgcgg cgagcaccgt cgtcaccgcc gcgcggttcg cggtgagcac
      301 cgtggattcc ggccgccaga catcggccgc gatggcggcg aggtgcgcgg tggagccacg
      361 cggcaggacc gggagcagga cctgcgtctc gggatcgccg aagcggcagt tgaactcgag
      421 cacccatggc gtgccgtcgg tgccgatcat cagccccgcg tacaagacgc cgctgtagcg
      481 gcagccgcgg cgtaccagct ccgccagggt cggtaccagc acctcgcgcc gcacccgttc
      541 cagcatcgcg ggtgtggcca ggctcaccgg gcaataggcg cccatgcccc cggtgttggg
      601 acccgtgtcg ccttctccca accgcttgtg gtcctgcgcg cccgggagga tcaggagccg
      661 ctcgccgtcg gtcagggcca ggaccgagag ctcctcgccc tcgaggaacg tctccacgac
      721 gatctcccgg ccggcttcgc cgaggacgcc cgactcgagc atgtcgcgcg ccaccgcggc
      781 ggcgtcgctg cgcgtcgcgc agaccaccgc ccccttgccg gccgcgagcc cggaggcctt
      841 gacgaccagg ggttcggcgt ggccctctat gtgcgccagc gcgggagcga tctcggtgaa
      901 cgtgcggctc gcggcggtcg gcacgccggc gctggccatg acgtctttcg caaaggactt
      961 ggaggattcg atccgggcgg ccgccgcgct ggggccgaac gcgggatgtc ccgtcgcccg
     1021 caggcggtcg accagccccg cggcgagcgg cacttcggga ccgatgacgg tgaggtcgat
     1081 gcggtgatca cggacggcgc cgacgacgcc gtccagatcg gtggcggcga cggcgagatt
     1141 ggtggcgagg gcggcggtgc cggggtttcc gggagcggca aagagggtcg tcgaggggtc
     1201 gtccgcgtgc agcgcccagc agagggcatg ctcgcggccg ccccccccga cgaccaggac
     1261 cctcacttct tcttcatggc gtccgagaac gcctgcgcgg cgcgcttggc cgagtcgacg
     1321 acgtcattga cgaccgcctt ggcttccttc atgtagtgct cgcgcgcatc gtccaggtcc
     1381 ttctccagcg ggcgctgctc gacgtccggc ccgcgccgcg ggtactcgcc gcgcagaatg
     1441 cggtcgtact cccccgcttc cacccagcgc tgcagctccg ccgcccgcac ggtattgaac
     1501 gggtgggtgc gcagcagcac gccgaggatc ttgaagaccc gatccatggc gcccccggtc
     1561 tcctcgtact ccttcgcctg gacgaggaag gcgttcaggt ccatctgctt catgtcgccg
     1621 ccgccggcga acttgaggtt catccgcagc gacgcgaccg ggtcctgcgt ggcgagcagg
     1681 ccggcacggt cggaggacag ctcgctcttg cggaaccact cgagcagggc gagctggatc
     1741 ggaaggatgg cgatcccggc gaggaagggg agcgccgaga ggctgacgtt caggagcagc
     1801 acgagcaggg tgtgatacag cgcgtggccg ctcatgacgt gtcccagctc gtggccgagc
     1861 agcgagcgca tctcgtcgtc gtccagcagg gtcagcgcgc cggaattgat cacgatgaac
     1921 ggcttgtcca tgccgaaggc gccggcgttc gcgaacggcg tctgggagac gaacagctcg
     1981 gggcgcaccg accagtccat ggtggtcagc acgtcggtgt agagctggtt gagccgcggg
     2041 aactgcgtgg gacccacgcg gacggcatcg gcctggaaca ggaggcggac gccgcgctcg
     2101 cccaggaagc cgtagatctt cttcaggacc tcgtcgaagc ccggcacgga gcggagcgat
     2161 tggagcgccg cccggtcggc gggatgctcc caggcgacgg gggcgatctg ggtgaggatc
     2221 ttgcggggcc gcgcggccgg caggcccccg ctggtggcat cggtcatgga agactccgca
     2281 gagttgaacg cttcactacg agccggtccg gcacgtgttt cgctagccgg cggcgagcgc
     2341 ctggtccagg tcctctatca gatcctcgat atcctcgatg cccaccgaca gccggatcag
     2401 actgtcggtg agccccatct tctgccgcag ctccggcggg acgctggcgt gcgtcatcga
     2461 cgcggggtgg ccgatcaggc tctccacgcc gcccagcgac tcggccaggg cgaacaccct
     2521 ggtgcgctcc accacccgtc gagcgaacgc ggggtcaccc agctcgaccg agagcatgcc
     2581 gccgaacccg ctcatctgcc ggcaggcgag gtcgtgttgt ggatgggagg ggaggcccgg
     2641 gtagtacacc tgccgtacgc gcggattgtg cgacagccac tcggccacgc ggcggccgtt
     2701 ggcgtcgtgc tggcgcatgc gcagcgggaa cgtcttgatg ccgcgcaagg ccagccagca
     2761 gtcgaacggc cccggcaccg caccggagcc gttctggatg aaggcgagcc gctcggccag
     2821 gtcgtcgcgc ggcgtcacga gcatcccgcc caccatgtcg ctgtggccgt tcagatactt
     2881 ggtggtcgag tggagcacga gatcggcgcc gaactcgagc gggcgctgga agcagggcgt
     2941 ggcgaacgtg ttgtccacga cgagcagcgc gccggcgccc ttggcgatct ccgccgtggc
     3001 ggcgagatcg cacaggcgca tcatcgggtt ggtcggagtc tcggcgtaga tcagcttggt
     3061 gcgcggggtc acggcggcgg cgaccgcatc cgcgttgcca ccgtccacga acgtgaacga
     3121 cagcccgagc cgggaccaga tgagcgtcat ctggcggtgg gtgccgccgt acacgttctc
     3181 accgctcacg acgtggtcgc ccgcggagag cagcttcatc accgcgtcca ccgcggcgag
     3241 accggagccg taggcgagcc cgtgccgtcc cccctcgagg gcggccacgc tgcgctccag
     3301 ggcttcgcgc gtcgggttgc gggtgcggcc gtactcgtag cccttgtggc gccccaatcc
     3361 ttcctgcacg taggtcgagg tctggtagat gggggtcatc accgccccgg agagcgggtc
     3421 gggcaccatg ccgccgtgca cggcccgcgt gcccatccgg cgccgcaaat ccgcgtcaaa
     3481 cgccttggtc atgggcggga acgtaccgac gcctttcgcc gcgagcaagc gacccttacg
     3541 ttagcccgtg atgacgggcc gaaacgggct ttcctgcctc gtggtggatg acgaaccgcg
     3601 gctgcgacgc gtgatcgccc acctgctcga gggcgacggg ttccgcgtgc gcga
//
LOCUS       DBNGZW010000385         4935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_35661, whole genome shotgun sequence.
ACCESSION   DBNGZW010000385 DBNGZW010000000
VERSION     DBNGZW010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_35661"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            96..1481
                     /locus_tag="AC16JC_09095"
     CDS             96..1481
                     /locus_tag="AC16JC_09095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5164891.1"
                     /translation="MSRRVLVVDDEKGVREALKQVLEFEDIEVQTCASGLDALQAYAE
                     FRPHLVFLDVKMQGMDGLEVLRRLREYDAHAQVVMISGHGTIQTAVEATQLGAYDFLE
                     KPLDTDRILLTLRNALQQLELAVENVRLRAEVEARYEIVGSSRAVKTVIERIEKVAPT
                     PARVLITGENGTGKELVARAIHDLSPRARGPFVEVNCAAIPAELIESELFGHIKGSFT
                     GAVSDRAGKFELADGGTLFLDEIGDMSLSAQAKVLRALQEGVISRVGSGKPLQVDVRV
                     IAATNKTLEQEIAAGRFREDLLYRLNVVPIEVPPLRVRRSDIPQLVQHFSDQLCASGR
                     TGLKPKEFDPAALKRLAAHDWPGNIRELRNAVERLLILTQAEVVTEQDVERMVGDGGR
                     TVAAGEAAATGAFLRAGTFEEFKQAAERAYLLAKLKEHDWNVSETARTLAMPRSNLYK
                     KIERYRLAREN"
     gene            1481..1933
                     /locus_tag="AC16JC_09100"
     CDS             1481..1933
                     /locus_tag="AC16JC_09100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164892.1"
                     /translation="MAKKDQERDWDRELKEVDRLLNQLPTHREEQLAAAAAPKRTPVA
                     PAAGASQDGGSRLGTWARVGLGLLVGIGMPFWPYSHGCGFKLLFYMVGVLAVVVAGMW
                     SGLSSWKRRMGLAHLLSQLLVIWGLVLAAAVVLPRVGYAKDAATWFCP"
     gene            2010..3500
                     /locus_tag="AC16JC_09105"
     CDS             2010..3500
                     /locus_tag="AC16JC_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013062221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR5164893.1"
                     /translation="MPENFKNFIGGEWVAPSTGAYFENRNPADRTDLIGCFPRSGPDE
                     VQRAVVSARRGFAQWSKTPAPLRGQVLQRVGELLVRRKEEIARAMTREMGKVLAETRG
                     DVQEGIDTAFYAASEGRRLFGHVVPSELRNKWAMSFRRPIGVAGIITPFNFPLAIPTW
                     KTFPALLCGNAVIFKPAEDVPHTGHLLVEVLLEAGLPPEVVQLVHGQGSVVGKGIIEH
                     PDVPVISFTGSTETGSVIGAVCGRMHKRLSLEMGGKNAMIVMDDADLDLALDGVLWGA
                     FGTTGQRCTATSRLVLHQKVHDRFLQMLVDRAGKLRLGPGLDERTEVGPMIHDEAREK
                     VDEYVGIGKQEGASVAVGGARAGGGKLEAGWFYQPTVLAGVKPGMRVEQEEIFGPVLS
                     VIKVGSLDEAVRVNNDVQYGLSSSLYTRDVNAAFRAMQDLDNGITYINAPTIGAEAHL
                     PFGGVKQTGNGHREGGWEVYEFYSETKVCYVDYSGQLQRAQIDTYE"
     gene            3597..3669
                     /locus_tag="AC16JC_09110"
     tRNA            3597..3669
                     /locus_tag="AC16JC_09110"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3630..3632,aa:Asn,seq:gtt)
     gene            3708..>4935
                     /locus_tag="AC16JC_09115"
     CDS             3708..>4935
                     /locus_tag="AC16JC_09115"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5164894.1"
                     /translation="MSAVPDSLLRRAAAPAGWALRAYWGTAVLLAAAAGIGLVAALPV
                     TALVGRLRAPLLRVADLGLGRSSLATGPNELRAAATTDLLQLLQLVTAGVLCVAALSL
                     LALAFARGGSRVAELAVRRAVGASRRLILVAALIEGGVIAAAALAWGGVGGAMGTHAA
                     LGAWPGTLRPGTVTPAVTVLAVVVSLVLLAVLLPLGSMRRRRPLATRSGRALELVVPA
                     LQLGLSLTVLAASALLADHAARLRGRDRVAAGGGRLFAISLATSDTVRAGSRYADLLR
                     GVSAQPGVTAASLTSPGATIGLGQNDRATTDCGQCAWDGIYVPLRPVYATHYLVSADT
                     FRTLGLRVLAGRNIANADDARAPLVAVVSQSLAAANFQDGRGVGRRIQVGSAPASWYT
                     VVGVVADPLPAGFGAGF"
BASE COUNT          740 a         1579 c         1828 g          788 t
ORIGIN      
        1 gactcgagcg gcgcggtggc gctgcgcgtg gatgccgcga tcgcggacgt ggcgatccat
       61 cccgcccgcg tcgttctgta ccgccggagg cgcccatgag ccggcgggtg ctggtggtgg
      121 acgacgaaaa gggcgtgcgc gaggccctga agcaggtgct ggagttcgag gacatcgagg
      181 tgcagacctg cgcgtccggc ctggacgccc tgcaggctta cgcggagttc cgaccgcacc
      241 tggtgtttct cgacgtcaaa atgcagggga tggacggact cgaagtcctc cggcggctgc
      301 gcgagtacga tgcccacgcc caggtggtga tgatctccgg ccacggcacg atccagacgg
      361 ccgtggaggc gacacagctc ggcgcctacg atttcctgga aaaaccgctc gacaccgatc
      421 gtatcctgct gacgctgcgc aacgcgctgc agcagctgga gctggcggtg gagaacgtgc
      481 gcctccgcgc cgaagtggag gcgcgctacg agatcgtggg gtcgagcagg gccgtcaaga
      541 cggtgatcga gcgcatcgag aaggtcgccc cgacgccggc ccgggtgctg atcacggggg
      601 agaacgggac ggggaaggag ctcgtggcgc gggcgattca cgacctgagt ccgcgcgcgc
      661 gcgggccgtt cgtggaagtg aactgtgcgg cgatcccggc cgagctgatc gagagcgagc
      721 tgttcggcca catcaagggc agcttcaccg gggccgtgtc ggatcgcgcg ggcaagttcg
      781 agctcgccga cggcggtacg ctgttcctcg acgaaatcgg ggacatgagc ctttcggcgc
      841 aggccaaggt gttgcgggcg ctgcaggagg gcgtgatttc ccgggtgggc tcgggaaagc
      901 cgctgcaggt ggacgtgcgt gtcatcgccg cgaccaacaa gacgctggag caggagatcg
      961 cggcgggacg gttccgcgag gatctcctgt accgcctcaa cgtggtcccg atcgaggttc
     1021 ctccgctgcg cgtccggcgc agcgacattc cccagctggt gcagcatttc agcgaccagc
     1081 tctgcgcctc gggccgcacc gggttgaagc ccaaggaatt cgatcccgcc gcgctcaaac
     1141 gtctggcggc gcacgactgg cccgggaaca tccgggagct gcgcaacgcc gtcgagcgtc
     1201 tgctgatcct cacgcaggcc gaggtcgtga cggagcagga cgtcgagcgc atggtgggcg
     1261 atggtggtcg caccgtggcg gccggcgagg ccgcggcaac gggtgcgttc ctgcgggccg
     1321 ggaccttcga ggagttcaag caggccgccg aacgggccta cctcctggcg aagctgaagg
     1381 agcacgactg gaacgtttcg gaaacggcgc gtacgttggc gatgccccgg agcaatctct
     1441 acaagaagat cgaacgctat cgtctcgcac gggagaacta gtggccaaga aggaccagga
     1501 gcgggattgg gacagagagc tcaaggaggt ggaccggctg ctgaaccagc tgccgaccca
     1561 ccgtgaggag cagctggccg cggccgcggc tccgaagcgg acgccggtcg cccctgccgc
     1621 gggcgcatcg caggacggcg gctcccgtct cggcacctgg gccagggtgg ggctcggcct
     1681 cctcgtcggc atcgggatgc ccttctggcc gtacagtcat ggctgcgggt tcaagctcct
     1741 tttctatatg gtcggggtgc tggcggtggt cgtggccggc atgtggagcg gcctgtcgtc
     1801 gtggaagcgc cgcatggggc tggcccatct gctctcgcag ctgctggtga tctgggggct
     1861 cgtcctggca gcggcggtgg tgctgccccg cgtcgggtat gcgaaggacg ccgcgacgtg
     1921 gttctgtccc tgaactgcgg ctatcggcta tcggccagat tcgaaccgac agccgatgac
     1981 cgatagccga tagccgatag cgagaatcta tgcccgagaa cttcaaaaat ttcatcggtg
     2041 gcgagtgggt ggcgcccagt acgggcgcct acttcgagaa ccgcaatccc gccgatcgca
     2101 ccgatctgat cggctgcttc ccgcgctcgg ggcccgacga ggtgcagcgc gccgtcgtat
     2161 cggcgcgccg cgggttcgcg cagtggtcga agacgcccgc cccgctgcgc ggacaggtgc
     2221 tgcagcgcgt cggcgaacta ctggtgcgcc gcaaggagga gatcgcgcgc gcgatgacgc
     2281 gggagatggg caaggtgctg gccgagacgc gcggcgacgt gcaggagggt atcgatacgg
     2341 ccttctacgc ggccagcgaa gggcggcggc tgttcgggca cgtggtgcct tcggagctgc
     2401 gcaacaaatg ggcgatgagc ttccggcgcc cgatcggcgt cgccggcatc atcaccccgt
     2461 tcaacttccc gctcgcgatc ccgacctgga agaccttccc ggcgctgctg tgcggcaacg
     2521 ccgtgatctt caagcccgcg gaagacgtgc cgcacacggg acacctgctc gtggaagtgc
     2581 tgctggaggc gggcctcccc ccggaagtcg tacagctggt gcacggacag ggctcggtgg
     2641 tcggcaaggg gatcatcgag caccctgacg tgccggtgat ctcgttcacc gggtccaccg
     2701 aaacggggtc cgtcatcggc gcggtctgcg gccgcatgca caagcgcttg tcgctcgaga
     2761 tgggcggcaa gaacgcgatg atcgtgatgg acgatgcgga cctcgatctg gcgctggacg
     2821 gcgtgttgtg gggcgcgttc gggaccacgg gccagcgctg cacggcgacg agtcggctcg
     2881 tgctgcacca gaaggtgcac gatcgctttc tccagatgct ggtggaccgc gccggcaagc
     2941 tccggctcgg gcccgggctg gacgaacgca ccgaagtcgg tcccatgatc cacgacgaag
     3001 cgcgtgagaa agtggacgag tacgtcggta tcgggaagca ggagggcgcc tcggtcgcgg
     3061 tcgggggtgc gcgggcgggc ggagggaagc tcgaggcggg gtggttctat cagcccaccg
     3121 tgctcgccgg ggtgaaaccg ggaatgcgcg tcgagcagga ggagatcttc ggtccggtac
     3181 tgtcggtgat caaggtgggc tcgctggacg aggcggtccg cgtcaacaac gacgtgcaat
     3241 acggactgtc cagctcgctc tacacccgtg acgtgaatgc cgcgttccgc gcgatgcagg
     3301 acctcgacaa cggcatcacc tacatcaacg cgccgaccat cggcgcggag gcgcatctgc
     3361 cgttcggtgg ggtgaagcag accggcaacg gccatcgcga aggcgggtgg gaggtgtacg
     3421 agttctattc ggagaccaag gtctgctacg tggactactc ggggcagctg cagcgcgcgc
     3481 agatcgacac atacgaataa ttgcggattg gcgattgcgg attgcggatt gaactgcacc
     3541 tcgctgggcg atcttcaatc cacaatccgc aatccacaat ccgcaattgt ctttcttggg
     3601 gtgtagctca actggcggag cgcccggctg ttaaccggga ggttggaggt tcgaatcctc
     3661 ccgccccagt ggttctctcc gccgccacat tccgccggag gccaacggtg agcgcggtgc
     3721 ctgatagcct gctccgacgt gcggcggccc cggccggctg ggcgctccgg gcatactggg
     3781 gaacggccgt gctgctggcc gccgccgcgg gcatcggcct ggtggcggcc ctgcccgtga
     3841 cggcgctggt ggggcggctg cgcgcgccgc tgctgcgggt cgcggatctg gggctgggac
     3901 gcagcagcct ggccaccgga ccgaacgagc tgcgtgcggc cgcgacgacc gatctgctcc
     3961 agctcctcca gctcgtgacg gcaggcgtgt tgtgcgtcgc cgcgctgtcg ctgctggcgc
     4021 tggcgttcgc gcgtggcggc tcgcgcgtcg ccgagctggc ggtgcgtcgc gccgtcggcg
     4081 cctcgcggcg gctgattctc gtggcggcgc tgatcgaagg gggcgtcatc gccgccgccg
     4141 cgctggcatg gggtggggta ggcggcgcga tgggaacgca cgccgccctc ggcgcctggc
     4201 ccggaacact gcggcccggc accgtgacgc ctgccgtcac agtcctggcg gtcgtcgttt
     4261 cactcgtgct cctcgccgtc ctcctgccct tgggatcgat gcgccgccgc cgcccgctcg
     4321 cgacgcgcag cggccgggcg ctggagctcg tcgttcccgc cctgcagctc ggcctctcgc
     4381 tcaccgtcct ggcggcctcc gcgctcctgg ccgatcacgc ggcgcgcctg cgcggccggg
     4441 atcgcgtcgc cgcgggtggt ggccggttgt tcgcgatctc gctggcgacc tcggacaccg
     4501 tccgggcggg gtcgcgctac gccgacctgt tgcgcggcgt gagcgcgcag ccgggtgtga
     4561 ccgccgcgag cctcacgagc cccggggcga cgatcgggct cggtcagaac gatcgagcca
     4621 ccaccgactg cgggcagtgt gcctgggacg gcatctacgt cccgcttcgt cccgtgtacg
     4681 cgacgcatta tctcgtcagc gccgacacct tccggaccct gggactccgg gtcctggcgg
     4741 ggcggaacat cgcgaacgcg gacgacgcgc gggcgccgct cgtcgccgtg gtgagccagt
     4801 cgctcgccgc ggcgaacttc caggatggac ggggcgtggg ccgccgcatc caggtgggga
     4861 gcgccccggc cagttggtat accgtggtgg gggtggtcgc cgatccgctg ccggcggggt
     4921 tcggcgccgg tttcc
//
LOCUS       DBNGZW010000386         8114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_172991, whole genome shotgun sequence.
ACCESSION   DBNGZW010000386 DBNGZW010000000
VERSION     DBNGZW010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8114
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_172991"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="AC16JC_09120"
     CDS             <1..329
                     /locus_tag="AC16JC_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011340124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HIR5164895.1"
                     /translation="VIARMDADVISIENARSGSELVRAFEQYRYPNEIGPGVYDIHSP
                     RVPAVAEIVTALRALGKVVDERQIWVNPDCGLKTRGWEETVPSLRNMVAAARQMRHEG
                     EGTRER"
     gene            375..980
                     /locus_tag="AC16JC_09125"
     CDS             375..980
                     /locus_tag="AC16JC_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020381662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR5164896.1"
                     /translation="MTAPEIRITAEPVDQQRCKFLVDRPVYQGVRRFASRAEAQGSPL
                     AEALYAIPGVSEIIVGANLVTVVKDPADPWPVVGKSVGAAIRRALAADTPPVADKAPA
                     PGAARLSDDELYERVNDLFEQQVNPMVARHGGRVELIDVQDAVVLLRMGGGCQGCGMA
                     SVTLRQGIEGMLTEHIPEVKGIVDITDHASGNNPYFASAKK"
     gene            1036..2223
                     /locus_tag="AC16JC_09130"
     CDS             1036..2223
                     /locus_tag="AC16JC_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012632801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5164897.1"
                     /translation="MEAARRQGVDLTVASEEPSTFEASRPDALLTLDFSHPAKAANQA
                     RAFAARYPVDGVLGVDDDTVVVAAVIAHALGVPGTSVAAAEAARDKYRQRELMGQGGV
                     PIPWFQLHRLDEDIDALARTVAYPCIVKPLRLSASRGVIRADGPQEFAAAVRRLAAIL
                     AQPDAAACADVADAFLVEEYIPGVEVALEGLLTAGALHVLALFDKPDPLDGPFFEETI
                     YATPSQLPETAQQALGDTAALAARAIGLTHGPIHAELRWNAKGPWLIELAARPIGGRC
                     SAALRFQDGATLEDVVIREALGLPLGSLLREDRAAGVMMIPVPGAGILREVRGVAEAR
                     EVPGIEDVVITAHRGQTLVPWPEGSRYPGFIFARATEAADVVTALRAAHRRLTFVRDR
                     QTD"
     gene            2227..3534
                     /locus_tag="AC16JC_09135"
     CDS             2227..3534
                     /locus_tag="AC16JC_09135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HIR5164898.1"
                     /translation="MRFPLPLDPWPRLGGERATRRAQLAIYALVLLVAVAEVVRSLGR
                     QHAVIFEGYVQVGDLVLSGGDPYGLRLNTWPPFFFFVAAVLSLVAKLSPTLALLVWQI
                     GDVFAIWGCCRLSVRLFAPDVPGPTFWPRDGQTAFGSADVLVPFLMTARLFQEHVQHT
                     QINAQILFLVLLAFTLFRERRLAAGGLSLAVAASTKAVPVLLLPYLVYKRAWRELGWT
                     AAFLLLLNLALPAAVFGPARTFGDWRTWRVVAARETADPTPHFMNQSFPAGLKRLLTD
                     AGSARDPIHYAVADWSTRAVQAVFFAAALAVALVLAYWFRRHPRDWADPRTAAELAIL
                     LGAMVVVDPLAWKAHYVVLIVPYTFAWWALRRLPADAPGRRWRWALWWGSFACITLSA
                     PTIVGVHWRDVLESLNVILIGALLLLVLAVSLVEAPKRARTST"
     gene            3531..4130
                     /locus_tag="AC16JC_09140"
     CDS             3531..4130
                     /locus_tag="AC16JC_09140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5164899.1"
                     /translation="MSRPERLTDEEQRGLLARLRAREEAALAELYDRLSPWVLGVAQR
                     ILGDADEADEVLSDVFVQVWRRIDQHDASRGPLTPWVLSIARNRSLDVLRRRGRWRRK
                     VALVEAEAKVDGDEPAAPADLAEASVPGWPVHQAVHQALAALPEEQRRVVLLAYFEGL
                     SHSEIAQRTGDPLGTVKTRLRLAQQKLEESLRSLKDWTV"
     gene            4127..4687
                     /locus_tag="AC16JC_09145"
     CDS             4127..4687
                     /locus_tag="AC16JC_09145"
                     /inference="COORDINATES: protein motif:HMM:NF021604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR5164900.1"
                     /translation="MSDWLGMASTPRRPRPELKARVLARAYATSRRPLAWPLAAAALL
                     LLGLAGGGVLWRRVKQLQSDLAATRDTLGLLRQPGTRVVAVPVVTQGRPGALTIFADS
                     TSHRWLVTCHNLTPNAPGEAYQLWFVTEQGMRTAALMPMDQSNPMVLAFDLPQGAGKV
                     MGMAMSVEPRAGSIEPKGPMVFHVSL"
     gene            complement(4690..5181)
                     /locus_tag="AC16JC_09150"
     CDS             complement(4690..5181)
                     /locus_tag="AC16JC_09150"
                     /inference="COORDINATES: protein motif:HMM:NF013440.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HIR5164901.1"
                     /translation="MLDELKARLQAEVERLNHEISFVLPKEIEKARAHGDLRENSEYK
                     AALERQQFAQARVHHLRLRLSKLSDVSDKDIPKDRVGFGSRVTVQDVETKAKETYALT
                     LGEFIEGDDNAQEMPVSMASPLGKALLGGKVGETVSITLPLGKRKLKILELVTVHEIA
                     RGA"
     gene            5267..6115
                     /gene="cyoE"
                     /locus_tag="AC16JC_09155"
     CDS             5267..6115
                     /gene="cyoE"
                     /locus_tag="AC16JC_09155"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR5164902.1"
                     /translation="MNGLFELSKPRITQLVVLTAAAGYYLGLRDAFQLPVFAATILGT
                     ALVASGTNAFNMIRERAVDARMRRTSRRPLPSGRVSLRAAVAFGTVTAAMGIGVLAWR
                     VNLLTAMLAALTLVSYVWFYTSLKRRSTLNTLVGAVPGALPVLGGWTAAGGTLDAPGL
                     ALFWTLFLWQLPHFLALAWMYREDYRSAQLKMLSVEDTDGRRTARMALLYAAALLPVS
                     LLPTLFGVTGTLYFWGALALGLAYAGAVVFMLVDVNRRAWWVFLASIIYLPALLTLAV
                     LDKVRG"
     gene            6127..7185
                     /locus_tag="AC16JC_09160"
     CDS             6127..7185
                     /locus_tag="AC16JC_09160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR5164903.1"
                     /translation="MGVFVLATAACTPRSASRGTEPAAALTLRLQSSGTTALLQAVSA
                     PSDRVAWVSGHSAAVLRTVDGGATWERIAVPGAAADSLQFRDVHAVDAEVAYLLAAGP
                     GSRSRIYKTTDGGGTWQLQFTNADSSAFYDCFAFWDASHGLAFSDAVAGHFVVRVTTD
                     GGAHWTPVTVDALPPAQPGEGAFAASGTCVVTLDPNQAWVGTGAADSARVLHTPDGGR
                     SWDVAVTPIAGGRTSGIATLAFRDPLHGIALGGNVADADARTDNVAVTPDGGRTWRVG
                     GRPGFSGAVYGAAIVPGRPGGVVAVGPKGLAYSTDDGANWTTVDTLAYWSVGFGSRRR
                     GWAVGPGGRITEMRLRLH"
     gene            complement(7207..>8114)
                     /locus_tag="AC16JC_09165"
     CDS             complement(7207..>8114)
                     /locus_tag="AC16JC_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase-like TK C-terminal-containing
                     protein"
                     /protein_id="HIR5164904.1"
                     /translation="DLTGSNLTQIKGSEDFAAGLVTGRNFHFGVREHAMGAVLNGLAY
                     HGGFLPFGGTFLIFSDYMRPAIRLAALARIKVIYVFTHDSIGLGEDGPTHQPIEQLAA
                     LRAIPNMTVIRPSDPGETVEAWRAAIRHQGGPVALVLTRQKVAVVDRTRHAAATGLHR
                     GGYALADAPDSLPEVVLLATGSEVAIALEAFDLLTKEGVRARVVSMPCLEYFARQPQS
                     YRDEVLPPAVTRRIAIEAAVPQSWYRWVGERGVVVGIDRFGASAPYQRIYREFGLTVE
                     NVVAQARSLLQPGQLDREALRRSGA"
BASE COUNT         1147 a         2755 c         2875 g         1337 t
ORIGIN      
        1 cggtcatcgc gcggatggac gcggacgtga tctcgatcga gaatgcgcgc tcggggtcgg
       61 agctggtgcg ggcgttcgag cagtaccgct accccaacga gattgggcct ggggtatacg
      121 acatccactc gccgcgggtc ccggcggtcg ccgaaatcgt gaccgcgctg cgggcgctcg
      181 ggaaggtggt cgatgaacgc cagatctggg tgaaccccga ctgcgggctc aagacgcggg
      241 gttgggagga aaccgtgccg tccctgcgga atatggttgc ggcggcaagg caaatgcggc
      301 acgagggaga agggacgagg gagcggtagg gcctcgtccc ccgtccctct tcccccgtcc
      361 cttatacttg gatcatgacc gctcccgaga tccgcatcac cgccgagccg gtcgaccagc
      421 agcgctgcaa gttcctcgtg gaccggccgg tctaccaggg cgtgcggcgc ttcgcgtctc
      481 gagccgaggc ccagggctca ccgctcgcgg aagccctgta tgcaatcccg ggggtcagtg
      541 agatcatcgt cggagccaat ctcgtcacgg tcgtgaagga cccggctgac ccctggccag
      601 tagtggggaa atcagtgggc gcggcaatcc gacgggcgct cgccgcggat acaccgcccg
      661 tggccgacaa ggcgcccgcg cccggcgccg cccgcctctc cgacgacgag ctgtacgagc
      721 gggtcaacga cctgttcgag cagcaggtca accccatggt cgcgcgccat ggggggcgcg
      781 tggagttgat cgatgtccag gacgcggtcg tgctgctgcg gatgggcggc ggctgtcagg
      841 gatgcggcat ggcgagcgtg acgctgcgtc agggcatcga agggatgctc accgagcaca
      901 ttccggaagt gaaagggatc gtggacatca cggaccacgc ctccggcaac aacccgtact
      961 tcgcttccgc caagaagtag tctgccccgt ctcctcctgc tgctcccgac cgccacgtac
     1021 cggaccaccg cgttcgtgga ggcggcgcgc cggcaggggg tggacctgac ggtcgcctcc
     1081 gaagagccga gcacattcga ggcgtcacgc cccgatgccc tgctcacgct ggacttctcg
     1141 catccggcga aggcggcaaa ccaggcgcgc gcattcgccg cgcggtaccc cgtggacggc
     1201 gtcctcggcg tggacgacga cacggtcgtg gtcgccgcgg tgatcgctca cgcgctcggc
     1261 gtccccggca cctccgtcgc ggcggcggag gctgcgcggg acaagtaccg ccagcgcgag
     1321 ctcatgggac agggcggcgt gccgatcccg tggttccagc tccaccgact cgacgaagac
     1381 atcgacgccc tcgcccgtac cgtcgcctat ccctgcatcg tgaagccgct gcggctctcc
     1441 gcctcccgcg gcgtgatccg cgccgacggt ccccaggagt tcgctgccgc ggtgcggcgc
     1501 ctcgccgcga tcctggcaca gcccgacgcg gcagcgtgcg cagacgtcgc cgacgccttc
     1561 ctcgtcgagg agtacatccc gggcgtcgag gtcgcactcg aaggcctgct cacggccggc
     1621 gcgctgcacg tgctggccct gttcgacaag ccggatccgc tcgacggtcc gttcttcgaa
     1681 gagacgatct atgccacgcc gtcgcagctg cccgagacgg cccaacaggc gctcggggac
     1741 actgcggcgc tggcggcgcg agcgatcggc ctcacgcacg gtccgataca cgccgagctg
     1801 cgctggaacg cgaaaggccc ctggctgatc gagctcgcgg cgcgccccat cggcgggcgg
     1861 tgcagtgcgg cgttgcgctt ccaggatggg gcaacgctcg aggacgtggt gattcgcgaa
     1921 gcactgggac tgccgctcgg atccctgctg cgtgaggacc gcgcggccgg tgtgatgatg
     1981 attcccgtgc cgggagccgg gatactgagg gaggtgcgcg gagttgccga ggcacgcgag
     2041 gtaccgggca tcgaagacgt cgtcattacg gcccaccgag gtcaaacgct cgtgccatgg
     2101 cccgagggtt cccgctatcc gggcttcatc ttcgcgcgcg ccacggaagc cgcggacgtg
     2161 gtgacggcgc tgcgcgcggc gcaccggcgg ctgactttcg tgcgggaccg ccagaccgac
     2221 tgacggatgc ggtttccgct cccgctcgat ccctggccgc gcctgggcgg agagcgcgcg
     2281 acccgacggg cgcagctcgc gatctacgca ctggtgctgc tcgtcgccgt cgccgaggtc
     2341 gtccgatcgc tcggccgcca gcatgcggtc atcttcgaag ggtacgtgca ggtcggcgat
     2401 ctcgtcctct cgggcggcga tccgtacggt ctgcgtctca acacctggcc gccattcttc
     2461 ttcttcgtcg cggccgttct gtcgctcgtg gcgaagctct ccccgacgct cgcactgctg
     2521 gtgtggcaga tcggcgacgt tttcgcgatc tggggctgct gccggctatc ggtgcgactc
     2581 ttcgcaccag acgtcccggg cccgaccttc tggccgcggg acggtcagac ggcattcggc
     2641 tcggcggacg tgctggtgcc gttcctgatg acggcgcggc tgttccagga gcacgtgcag
     2701 cacacgcaga tcaacgcgca gatcctgttt ctcgtgctgc ttgcgttcac gctgttccgg
     2761 gagcgacgcc tggctgcggg cggactgtcc ctcgccgtgg cggcgagcac caaggcggtc
     2821 cccgtgctgc tgctgccgta tctcgtctac aagcgggcgt ggcgcgagct ggggtggact
     2881 gcggcctttc tactcctcct gaacctcgca ctgcccgcag ccgtgttcgg ccccgcgcgc
     2941 accttcggcg actggcgcac gtggcgggtg gtcgcggcgc gggagaccgc cgatccgacg
     3001 ccccatttca tgaaccagtc gttccccgcg ggactgaagc ggctgctcac cgacgcggga
     3061 agcgcgcggg acccgattca ctatgcggtg gccgactggt ccacccgcgc cgtccaggcg
     3121 gtgttcttcg cggccgcgct ggcggtggcg ctcgttctcg cctactggtt tcgccgccac
     3181 ccacgcgatt gggccgatcc acgtacggcc gcggaactcg caatcctgct gggcgcgatg
     3241 gtcgtggtgg atccactggc gtggaaggca cattacgtcg tgctcatcgt gccgtatacg
     3301 ttcgcctggt gggcgctgcg ccgtctcccg gcggacgctc caggccggag gtggcgctgg
     3361 gcgctgtggt gggggtcctt cgcctgcatc acgctcagcg cccccacgat cgtcggcgtg
     3421 cactggcgcg acgtgctgga atcgctcaac gtcattctca tcggagccct gctactcctg
     3481 gtgctggcgg tgtcgctggt cgaggcccca aagcgagccc ggacatctac gtgagccggc
     3541 cagagcgtct tactgatgag gaacaacgcg gcctcctggc ccgcctgcgg gcgcgcgagg
     3601 aagcggcgct ggcggagcta tacgatcgcc tgtccccatg ggtgctgggg gtggcgcagc
     3661 gcatcctcgg cgatgccgac gaggccgacg aggtgttgag cgacgtgttc gtgcaggtgt
     3721 ggcggcgcat cgaccagcac gacgcctcgc gcggcccctt gacgccctgg gtgctctcca
     3781 tcgcccgcaa ccggtcgctc gacgtgctgc gccggcgcgg gcggtggcga cgcaaggtgg
     3841 cgctggtcga agccgaggcg aaggtggatg gcgacgagcc ggcggctccg gccgacctcg
     3901 ccgaagcgtc cgtgcccgga tggccggtgc atcaggccgt gcaccaggcg ctggcggcgc
     3961 tccccgagga gcagcgacgg gtggtgctgc tggcctactt cgaggggctg tcacactcgg
     4021 agatcgcgca gcgcaccggc gatccgctgg ggacggtgaa gacgcggttg cgcctcgcgc
     4081 aacagaagct ggaggagagt ttgcgatcct tgaaggactg gacggtatga gcgattggct
     4141 gggtatggct tcgactccgc gacgcccgcg gcccgagctg aaggcacggg ttctcgcccg
     4201 ggcgtacgcc acgtcgcgac gcccgctggc gtggccactc gcagccgcgg cgctcctgct
     4261 gctcgggctc gcgggtggtg gcgtgctgtg gcgtcgcgtc aagcaactcc agtccgacct
     4321 cgccgcgacg cgcgataccc tcggcctgct gcgccagccg gggacacgtg tcgtcgccgt
     4381 cccggtcgtc acgcagggcc gccccggcgc gctcaccatt ttcgccgaca gcacttcgca
     4441 tcgctggctc gtgacctgtc acaacctgac gcccaacgct ccgggcgagg cctaccagct
     4501 gtggttcgtc accgagcagg ggatgcgtac cgcggcgctg atgccgatgg atcagtccaa
     4561 tccgatggtg ctggcgttcg acctgccgca gggcgcgggt aaggtgatgg gcatggcgat
     4621 gagcgtcgaa ccgcgggcgg gatcgatcga gccgaagggc ccgatggtgt ttcacgtaag
     4681 tctgtagcgc tacgctcccc gggcgatctc gtggacggtc acgagctcca ggatcttcag
     4741 cttgcgcttt cccagcggca gggtgatgga cacggtttcg ccgactttgc caccgagcag
     4801 cgccttgccg aggggcgagg ccatcgacac cggcatctcc tgggcgttgt cgtctccctc
     4861 gatgaactcg cccagcgtca gggcgtaggt ctccttcgct ttcgtctcca cgtcctgcac
     4921 ggtcacgcgc gagccgaatc cgacgcggtc cttcgggatg tccttatcgg acacgtccga
     4981 cagtttggac aggcgcaggc gcagatggtg cacgcgcgcc tgggcgaact gctggcgctc
     5041 gagcgcggcc ttgtactcgg agttttcgcg caggtcgccg tgggcgcgcg ccttctcgat
     5101 ctccttcggt agaacgaagg agatctcgtg gttcaagcgt tcgacctcgg cctggaggcg
     5161 ggccttcagt tcatcgagca tggcgtgagg ggtggaaact aggcgacagt acgcgatgat
     5221 gccactactg gtgggacatc ggcagcctgg gcgacatttc cccccggtga acggcctctt
     5281 cgagctttcc aaacctcgca tcacgcaact cgtggtgttg accgcggcgg cgggctacta
     5341 cctcggcctg cgcgatgcgt tccagctccc cgtcttcgcg gcgacgatcc tcggcaccgc
     5401 cctcgtcgcg tccgggacca acgcgttcaa catgattcgc gagcgcgcgg tggatgcccg
     5461 gatgcgccgc accagccgcc gtccgttgcc gagcgggcgc gtgtcgctgc gggcggcggt
     5521 ggcgttcgga accgtcaccg ccgcgatggg gatcggcgtg ctcgcgtggc gggtgaacct
     5581 cctcaccgcg atgctcgccg ccctgacgct ggtcagctac gtctggttct acacgtcgct
     5641 caagcgacgc agtacgctca acacgctggt gggagccgtt ccgggcgctc tccccgtgct
     5701 cggcgggtgg acggccgctg gcggcacgct cgacgccccc ggcctcgcgc tcttctggac
     5761 gctgttccta tggcagctgc cgcacttcct cgccctcgcc tggatgtatc gcgaggacta
     5821 ccgcagcgcg cagctcaaga tgctgtcggt ggaagacacg gacggccggc gcacggcgcg
     5881 gatggcgctg ctctatgcgg cggcgctgct ccccgtgagc ctgctcccga cgctgttcgg
     5941 cgtcaccggg accctctact tctggggcgc tctcgcactc ggtctcgcct acgccggcgc
     6001 cgtcgtcttt atgctggtgg acgtgaaccg gcgggcctgg tgggtgttcc tcgccagcat
     6061 catctatctg cccgcgctgc tgacgctcgc ggtgctcgac aaggtcagag gctgacgctg
     6121 cggtggttgg gggtgttcgt cctggccacc gcggcgtgca cgccgcggag cgcgagccgc
     6181 ggcacggagc cggctgccgc gctcacgctc cgactgcagt cgagcggcac gaccgcgctg
     6241 ctgcaggcgg tgagcgcccc gagcgaccgg gtcgcctggg tgagcggcca ctccgccgcg
     6301 gtgctgcgga ccgtggacgg cggcgccacg tgggagcgca tcgccgtgcc gggagcggcg
     6361 gcggactcgc tgcagttccg cgacgtgcac gcggtggacg cagaggtcgc ctacctcctg
     6421 gccgccgggc cggggagccg ctcgcgcatc tacaagacca ccgacggcgg cggcacctgg
     6481 cagctgcagt tcacgaacgc ggactcgagc gccttctacg actgcttcgc gttctgggac
     6541 gcatcgcacg gcttggcctt cagcgatgcc gtggcaggcc acttcgtggt ccgcgtgaca
     6601 acggacggcg gggcccactg gactcccgtt acagtggacg cattgccccc cgcgcagccg
     6661 ggggagggcg cgttcgcggc aagcggcacg tgcgtcgtca cactcgaccc gaaccaggcg
     6721 tgggtgggca ccggagcggc cgacagcgcg cgcgtgctgc acacgcctga tggagggcgc
     6781 tcctgggacg tcgcggtaac gccgatcgcg ggaggacgca catcgggcat cgcgacgctc
     6841 gcgttccgcg atccgcttca cgggatcgct ctgggcggga atgtcgccga cgccgacgcg
     6901 cggaccgaca acgtggcggt cacgccggac ggtggccgga cctggcgagt gggtggccgg
     6961 ccggggttca gcggagcggt gtacggcgcg gcgatcgtcc ccgggcgtcc ggggggggtg
     7021 gtcgcggttg gccccaaggg gctcgcctac tcgaccgacg atggcgcgaa ctggacgact
     7081 gtggacacgc tggcgtactg gagcgtgggc ttcggctcgc gacggagagg gtgggccgtt
     7141 gggccgggag gccgaataac cgagatgcga cttaggcttc actgagtggc tcaggtctca
     7201 caaccgctac gcgcccgagc gtctgagcgc ctctctgtcc agctgtccag gttgaagcag
     7261 cgacctcgcc tgtgcaacca cgttctccac cgtcaatccg aattctctgt agatccgttg
     7321 atagggcgcc gacgctccga accgatcgat ccccacgacc actccgcgct caccgaccca
     7381 gcggtaccac gactgcggca ccgccgcttc gatggcgatg cggcgagtga cggcaggggg
     7441 gagcacctca tcacggtagc tctgcggctg ccgcgcgaag tactcgagac atggcatgct
     7501 gacgactcgt gcgcggaccc cttccttggt cagcagatcg aaggcttcga gggcgatcgc
     7561 cacttccgag ccggtggcga gcagtacgac ttcgggcagg ctgtcgggcg cgtccgcgag
     7621 cgcgtacccg ccgcggtgca ggccggtggc ggcggcgtgc ctggtgcgat cgaccaccgc
     7681 caccttctgg cgggtgagca ccagggcgac cggcccgccc tggtggcgga tcgcggcccg
     7741 ccacgcctcc accgtttcac ccgggtcgga gggccggatc accgtcatgt tggggatcgc
     7801 gcgcagggcg gcgagctgct cgatcggctg gtgggtgggg ccgtcctcgc ccaagccgat
     7861 cgaatcgtgc gtgaacacgt agatgacctt gatgcgcgcg agggcggcca gccgaatcgc
     7921 agggcgcatg tagtccgaaa aaatcaggaa cgtcccgccg aacggaagga atcccccgtg
     7981 ataggcgaga ccgttcagca ccgcgcccat ggcgtgctcg cgcacgccga aatggaagtt
     8041 gcgcccggtg acgaggccgg cggcgaagtc ctcggatccc ttgatctgcg tcaggttgga
     8101 tccggtgaga tccg
//
LOCUS       DBNGZW010000387         1354 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1127746, whole genome shotgun sequence.
ACCESSION   DBNGZW010000387 DBNGZW010000000
VERSION     DBNGZW010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1354)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1354)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1354
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1127746"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="AC16JC_09170"
     CDS             complement(<1..287)
                     /locus_tag="AC16JC_09170"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5164905.1"
                     /translation="MDTRGPEGAGSEYVERARALAPRIETCADQIERERRLAEPVLDA
                     LFAAGMFRLLLPRSLGGGEVDPATFAAVIEEIAKADASTAWCMCQASGCSMS"
     gene            379..1242
                     /locus_tag="AC16JC_09175"
     CDS             379..1242
                     /locus_tag="AC16JC_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018458262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5164906.1"
                     /translation="MKRYDRHQQDVGNILMLEHVNVRVPDQTIATAFYVQGLGFTRDP
                     YIMVGVENMWINMGQTQFHLPTGKPNVMRGHVEIVVPDLEALTARLGSVRALLTDTKF
                     DYSVEDKYVAVTSPWGNRLRLYAAGPAFGDWTLGITNVEFPVEPGRAAGIARFYEKVM
                     GAPAAVTPDADGAAARVRIGQRQELVFRETTAPIPPYDGYHIAIYIANFSGPHEWLDS
                     HGLVSEESQDYQYRFEKIVDPDTCECLCVIEHEVRSYTHPMFLRPLVNRNPAQRQATY
                     QRGRDAFYPTG"
BASE COUNT          240 a          482 c          437 g          195 t
ORIGIN      
        1 gacatcgagc acccggacgc ctggcacatg caccatgccg tgctcgcgtc ggccttggcg
       61 atctcctcga tgacggcggc gaaggtggcg ggatcgacct cgcccccgcc gagtgagcgg
      121 ggcagcagca gccggaacat gcccgccgcg aacagcgcgt ccagcaccgg ctcggccagg
      181 cgccgctccc gctcgatctg atccgcgcag gtctcgatcc ggggcgcgag cgcgcgcgcc
      241 cgctccacgt actcgctccc cgctccctcc ggaccgcgcg tatccatggg cgggactata
      301 tcgcaaaacc ctccgtgtgg cttccggcgg cgtgctgggc taggataggc agcacactcg
      361 ccgccgagga ggatcgccat gaagcgctac gaccggcacc agcaggacgt cggcaacatc
      421 ctcatgctgg agcacgtgaa tgtgcgcgtg cccgaccaaa cgatcgccac ggctttctac
      481 gtccagggtt tgggcttcac gcgcgacccc tacatcatgg tcggggtcga gaacatgtgg
      541 atcaacatgg gccagacgca attccatctg ccgaccggca agccgaacgt catgcgcggt
      601 cacgtcgaga tcgtggtccc cgatctcgag gcgctcaccg cgcggctggg aagcgtccgc
      661 gccctgctca cggacacgaa gttcgactac agcgtcgagg acaagtacgt cgcggtgacg
      721 tctccatggg gcaacaggct gcgcctctac gctgccggcc ccgcgttcgg agactggacc
      781 ctcggcatca ccaacgtgga attcccggtc gagcccgggc gggcggccgg catcgcgcgc
      841 ttctatgaga aggtcatggg cgcccccgcg gcggtcaccc ccgacgcgga cggcgcggcc
      901 gcgcgcgtca ggatcggcca gcgtcaggag ctcgtcttcc gggagacgac ggcgcccatc
      961 ccgccttacg acggttatca catcgcgatc tacatcgcga acttctccgg cccccacgag
     1021 tggctcgaca gccacgggct cgtcagcgaa gagagccagg actaccagta ccgcttcgag
     1081 aagatcgtgg atcccgacac gtgcgaatgc ctgtgcgtca tcgagcacga ggtgcggagc
     1141 tacacgcacc cgatgttcct tcggccgctc gtgaacagga acccggccca gcgccaggcg
     1201 acgtaccagc gaggccgcga cgccttctac ccaaccggct agcggccgcc tggagtcagg
     1261 ccgatcggat ggcgccgagc gccccggaca atagcgggaa tctgacgaag gtgcacccga
     1321 tgtaatgaac cacgccccgg ggctggcgcc ggtc
//
LOCUS       DBNGZW010000388         3458 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1605285, whole genome shotgun sequence.
ACCESSION   DBNGZW010000388 DBNGZW010000000
VERSION     DBNGZW010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3458)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3458)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3458
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1605285"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1710
                     /locus_tag="AC16JC_09180"
     CDS             <1..1710
                     /locus_tag="AC16JC_09180"
                     /inference="COORDINATES: protein motif:HMM:NF036968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR5164907.1"
                     /translation="GVVIVPDALSNYVPVQYAAKEIDNGKGVLPVPAIQFEKDGTEDA
                     GLVKIDLLGNRSLAVIRDAIAAVRAHEGVTIDYTTSAPAADEATQRVFRTGDTMGVFY
                     TESPASRLLCQRSKSDTFDLLVLNTSIIRPASNRYINTYLERLHGAPWEPPHPVLRDV
                     LADTFGVMVYQEDVVNVCVALAGMDVAEADGVRKALTKKRPMKELRAYEEQFRAGAGA
                     RGVDPKIVDDVWSMILSFSGYSFCKGHSASYIQVALQSGYLRAHHPAEFIAGVLSNEG
                     GYYTPFAYIAEARRMGLEILPPDVNASDVSYLGRGRTLRVGLMALKGLSAAGLGALVA
                     DRQHGGPYRSFSDLQQRAGLTPGDLRVLVQAGACDGIAQGRTRPQLMWIVDAGGARTP
                     GRGAPGRGSGVNPLQQEALALEETPAAALPPLAEYDAERQLRDEYAVLGYLASCHPMD
                     LVADTVRAVHAVPATALREHVGKLVTCVGMLTTGKPVHTIKDEPMEFVTFDDGHGLIE
                     TVIFPDVYRKVALLLFGTGPYVMRGNVEESYGAVTLTVTALDRLDRYAQRRSLPWRAL
                     SVT"
     gene            1731..2708
                     /locus_tag="AC16JC_09185"
     CDS             1731..2708
                     /locus_tag="AC16JC_09185"
                     /inference="COORDINATES: protein motif:HMM:NF014017.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HIR5164908.1"
                     /translation="MSPREEAAQPDLFGGGAAPLPEPPPGVVDLARGIPPNVRFGTST
                     WTYDGWAGDVYHRPYKGAQPARRLEEYAHYPLFRTVGIDSAFYDPPSEETLAAYAKSL
                     PPGFPCVSKVWDRITARRFNQDRRWGNLAGLRNPDFLNAQLFKDAVLLPYARAFRDHA
                     GCFVFEFQAMRGKDLPDIADWADDLNGFLEQLPRDFRYGVELRNPELLHDLHGTVLQR
                     HGVAHVFNSWTEMPPIGAQLDLPWTFPATFTVARALLKPGRKYEDAVRLFQPYERIRE
                     PQPDLRRDLMRLMAEAVRRRIEALIVVNNRAEGNAPGTIRALAEGYARV"
     gene            2705..3448
                     /locus_tag="AC16JC_09190"
     CDS             2705..3448
                     /locus_tag="AC16JC_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007176136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HIR5164909.1"
                     /translation="MKARTGRRWATDDARVARVNAIDWTTTCAHLDEHGWALLEGTLP
                     PDACRDAAGWYDDDSRFRSHVLMARHGFGRGEYKYFRYPLPSVIADLRTALYARLAPH
                     ANRWNDSLGSDVRFPEQHADFLERCHQAGQTRPTPLLLRYVAGDYNCLHQDLYGEHVF
                     PFQVAFLLSEPAQDFTGGEFVLVEQRPRMQSRVEVVPLRRGDGVIFAVHHRPVRGGRG
                     VYRVNLRHGVSRVRSGRRHTVGVIFHDAT"
BASE COUNT          545 a         1142 c         1227 g          544 t
ORIGIN      
        1 ggcgtcgtga tcgtccccga tgcgctcagc aactacgtcc cggtgcagta cgcggcgaaa
       61 gagatcgaca acggaaaggg cgtactgccc gttcccgcta ttcaattcga gaaagacggc
      121 accgaagacg cggggctcgt gaagatcgac ttgctcggca accgctcgct cgccgtgatc
      181 cgcgacgcga tcgcggcggt acgcgcgcac gagggcgtga cgatcgacta caccacgagc
      241 gcgcctgcgg ccgacgaggc gacgcagcgg gtgttccgca ccggcgacac gatgggcgtg
      301 ttctacacgg agtcgcccgc gtcgcggctg ctgtgccagc ggagcaagtc cgacaccttc
      361 gatctgctgg tgctcaacac gagcatcatc cgtcccgcct cgaatcgcta catcaacacc
      421 tatctcgagc ggctgcacgg cgcgccctgg gagccaccgc atccggtgct gcgcgacgtg
      481 ctggccgaca cgttcggcgt gatggtgtac caggaggacg tcgtcaacgt gtgcgtggcg
      541 ctcgcgggaa tggacgtcgc ggaagccgac ggtgtgcgca aggcgctgac caagaagcgg
      601 cccatgaagg agctgcgcgc ctacgaggaa cagttccgcg ccggcgcggg cgcgcgcggc
      661 gtcgatccga agatcgtgga cgacgtgtgg tcgatgatcc tctcgttttc cggctactcg
      721 ttctgcaagg gacactcggc gtcctacatc caggtggcgc tgcagtccgg ttacctgcgg
      781 gcgcatcatc ccgcggagtt catcgccggc gtgctctcca acgagggcgg ctattacacc
      841 ccctttgcct acatcgccga ggcgcgccgc atggggctcg agatcctgcc gcccgacgtc
      901 aacgcgagcg acgtgtctta tctcggacgg gggcgcacgc tgcgcgtcgg gctgatggcg
      961 ctgaaggggc tgtccgccgc cgggctggga gcactggtgg ccgaccgcca gcacggcggc
     1021 ccctatcgtt cgttcagcga cctgcagcag cgcgccgggc tcaccccggg cgatctccgc
     1081 gtgctggtcc aggccggtgc atgtgacggc attgcacagg ggcgtactcg accccagctc
     1141 atgtggatcg tggatgcggg gggggcacgg accccggggc gcggcgcccc ggggcgtggc
     1201 tccggggtga acccactcca acaggaagcg ctagcactgg aggagacgcc ggctgcggct
     1261 cttccaccac tcgccgagta cgacgccgag cggcagctgc gggacgagta cgcggtgctc
     1321 ggctatctcg cctcgtgcca tcccatggat ctggtcgcgg acacggtgcg cgccgtccac
     1381 gcggtgccgg cgacggcgct ccgggagcac gtgggcaagc tcgtgacctg cgtcggcatg
     1441 ctcacgacgg ggaagccggt gcacacgatc aaggacgagc cgatggagtt cgtgacgttc
     1501 gacgacggcc acgggctcat cgaaacggtg atcttccccg acgtataccg caaagtggcg
     1561 ttgctgctct tcggaacggg gccttacgtg atgcgcggga acgtggagga gtcctacggc
     1621 gcggtgacgc tcacggtcac cgcgctcgac cggctggatc gctacgcaca gcggcgcagc
     1681 ctgccgtggc gcgccttgag cgtcacgtga ggcaaccggt agactacggc atgagtccac
     1741 gcgaagaggc ggcgcaaccc gatctgttcg gggggggggc cgcgccactc cccgagccgc
     1801 ctcccggggt cgtggacctg gcgcgcggga tcccgccgaa cgtccgcttc ggcacgtcca
     1861 cctggaccta cgacggctgg gcgggggacg tctatcaccg tccctacaag ggtgcgcagc
     1921 cggcccgccg gctcgaggag tacgcgcact atccgttgtt tcgtacggtg ggtatcgaca
     1981 gcgcgttcta cgacccgccg agcgaggaaa cgctggcggc gtacgcgaag tcactgccgc
     2041 cgggcttccc atgcgtgagc aaggtgtggg accggatcac cgctcgccgc ttcaaccagg
     2101 accgccgctg gggcaacctc gccggcctcc ggaaccccga tttcctgaac gcgcagctct
     2161 tcaaggacgc cgtgctgctt ccctacgcgc gggcgttcag ggatcacgcc gggtgcttcg
     2221 tgttcgagtt ccaggcgatg cgcggcaagg acctgcccga catcgccgac tgggcggacg
     2281 acctgaacgg gttcctcgag cagctgccgc gggattttcg ctacggcgtg gagctgcgca
     2341 acccggagct gctgcacgat ctgcacggca cggtgctgca acggcacggc gtggcgcacg
     2401 tgttcaactc ctggaccgag atgccgccga tcggcgcgca gctcgatctg ccgtggacgt
     2461 ttccggccac tttcacggtg gcgcgggccc tgctcaagcc ggggcgcaag tacgaggacg
     2521 cggtgcggct gtttcagccg tacgagcgga tccgggagcc gcagcccgat ctacggcggg
     2581 atctgatgcg gctcatggcg gaagccgtgc gacggcgaat cgaggcgctg atcgtggtca
     2641 acaaccgggc ggaggggaac gcgccgggga cgattcgggc gctggcggag gggtacgccc
     2701 gagtttgaag gcaagaaccg ggaggcgctg ggcgacagac gacgctaggg ttgcccgtgt
     2761 gaacgccatc gactggacga cgacctgcgc ccacctcgac gagcatggtt gggcgctgct
     2821 cgaaggaacc ctgccgcccg acgcgtgccg ggatgcggcc gggtggtacg acgacgacag
     2881 tcggttccgc agccacgtac tcatggcgcg gcatgggttc gggcggggcg agtacaagta
     2941 cttccgctat ccgctgccaa gcgtcatcgc ggacctgcgc accgcgctgt acgcccgcct
     3001 tgccccacac gcgaaccgct ggaacgactc cctcggcagc gacgtgcgct ttcccgagca
     3061 gcacgccgac ttcctcgagc ggtgccacca ggccgggcag acccggccga cccccttact
     3121 gctgcgctac gttgcggggg actacaactg cctgcaccag gacctgtacg gggagcacgt
     3181 cttccccttc caggtggcgt ttcttctgtc ggagcccgcg caggacttca ccggcgggga
     3241 gtttgtgctg gtcgagcagc ggccgcggat gcagtcacgg gtggaggtgg tgccgttgcg
     3301 gcggggtgac ggggtgatct tcgcggtcca ccatcgtccc gtccggggcg gtcggggggt
     3361 ctatcgggtc aatctgcggc atggggtgag ccgcgtgcgg agcggccgcc gccacacggt
     3421 gggcgtgatc tttcacgacg cgacctgacg cgactagg
//
LOCUS       DBNGZW010000389         3872 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_240806, whole genome shotgun sequence.
ACCESSION   DBNGZW010000389 DBNGZW010000000
VERSION     DBNGZW010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_240806"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            46..139
                     /locus_tag="AC16JC_09195"
     tRNA            46..139
                     /locus_tag="AC16JC_09195"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:80..82,aa:Sec,seq:tca)
     gene            287..646
                     /locus_tag="AC16JC_09200"
     CDS             287..646
                     /locus_tag="AC16JC_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164910.1"
                     /translation="MTNERLYARLREDVNIRLRRGAWYRVLKVEGLKAILLVGGRHVL
                     VAAALLEIVKRPPPRWTVVTQTPSTARKAPRALAERYGVCPSCGDRAALAGRVRRLRC
                     GSCKFEFEVAWDEGYLV"
     gene            535..1590
                     /locus_tag="AC16JC_09205"
     CDS             535..1590
                     /locus_tag="AC16JC_09205"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR5164911.1"
                     /translation="MPQLRGPGGARRPGAAAALWLLQVRIRGRLGRGLPRLTPGSAER
                     GASVADRPALAHAYVILNPAAGRGAARRAVDAVARAFRHQGWTIDMMETARPGDGTAL
                     ASAAARAGARCVVAVGGDGAVHEVANGLLAVRSQAVLGVVPIGSGNDFAKLVGVYRHD
                     VARAVARLTRAVVRRFDAGRALDEYFVNTLGFGFGAAVVRTRNAMPGLKGFLSYLIPI
                     FRTFANFRPPVFEVRSAGFEEQGRMMMVEVCNGTTAGGDYRFAPDADPADGLLDVCLI
                     RRVGLVRFVRSLPHVMRGTHGSLKDIVALFRSASLTIRAPEAPLVLHLDGELREPGVH
                     ECTVTVEPKCLNVLVAK"
     gene            1587..2096
                     /locus_tag="AC16JC_09210"
     CDS             1587..2096
                     /locus_tag="AC16JC_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HIR5164912.1"
                     /translation="MKRRTGGQAVRRSRLRRAGVLSFVLLALSACPPERLTAQVPESL
                     PQPPAPRDTSHQVGPVGALWRSLVLPGWGQAATGRKVTGALFTVWEGTTMFMTLKASQ
                     EANYLRVSGAPNEQSKRQEVQDWLVLWGFNHLFSGAEAYVAAHLRDFPRDLKIRAVPG
                     GVGVRFSLP"
     gene            2100..2897
                     /gene="murI"
                     /locus_tag="AC16JC_09215"
     CDS             2100..2897
                     /gene="murI"
                     /locus_tag="AC16JC_09215"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014399032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR5164913.1"
                     /translation="MNAAPLGVFDSGIGGLTVAHALFECLPHESVIYFGDTARVPYGP
                     KSPETVRRYSGEILDFLVRRGVKMVVVACNTSTAHALEFLRSRSPVPVQGVIEPGARA
                     AAAATRAGGIGVIGTAGTIASGAYERAIRAVRPEVEVASQACPLFVPLVEEGWFDHPA
                     TELVAREYLQPLKDRQVDVLVLGCTHYPLLKPLLSRVMGPEVTLIDSAAETARTVRQQ
                     LEGQGLLAPPDSRSAHRFVVSDDEALFRRVGARFLGAKLQDVELAPL"
     gene            complement(2894..3634)
                     /locus_tag="AC16JC_09220"
     CDS             complement(2894..3634)
                     /locus_tag="AC16JC_09220"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HIR5164914.1"
                     /translation="MSPPAARCYVLADAHLGQVPANVEAALHAFLETVPQPGDELLVV
                     GDLFDFWFEYGRVVPRRHAVTAMKLGQLVTRGIPVTFLGGNHDRWGGNFLRQDLGIAY
                     HSGAAEVDLAGRRALAVHGDGLTEQHWGGTLTHWLLRQPLTITVFRTLHPAIGFWIAD
                     KLSGTLADATKDEAVLDRAAAAQASYAKAVLERRPELSLVVMAHTHRARLERLSDGRT
                     YLNPGAFLDGGRYAVVTPETVELKSFGV"
BASE COUNT          572 a         1321 c         1365 g          614 t
ORIGIN      
        1 ttgaagacag gcggaactct tggtgaacag aaggtgaaaa ccgacggggg cggatcgggc
       61 ctggtggcct gactggtctt caaaaccagc tggggttcgc aagggcctcg gtaggttcga
      121 ttcctacacg ctcccgccat ccccccgccc gccggatgtt cccagtgtgc gctccatcac
      181 ggggcgcggc ccggattccc ggcatattgg cgccccccgc tcgggcggcc ggtgcccgcc
      241 ccgtgggttt accactcgcc ttgggacccc cattattcgc ccggacatga cgaacgaacg
      301 cctctatgcc cgtctgcgcg aggatgtcaa cattcgcctg cgacgggggg cttggtaccg
      361 cgtgctcaaa gtcgaaggat tgaaggccat tctcctggtc gggggccgtc acgtgttggt
      421 ggctgcggca ctgctcgaga tcgtgaaacg ccccccgccg cgttggacgg tggtcaccca
      481 gactccgagt accgccagaa aagcgccgcg cgcactcgcc gagcgctacg gcgtatgccc
      541 cagttgcggg gaccgggcgg cgctcgccgg ccgggtgcgg cggctgcgct gtggctcctg
      601 caagttcgaa ttcgaggtcg cctgggacga gggctacctc gtctgacgcc ggggtcggct
      661 gagcgcggcg cgtcggtcgc ggaccggccc gcgctggctc atgcctacgt catcctcaat
      721 cctgcagccg gacggggcgc ggcacgccgc gccgtggacg cggtcgcccg ggcgttccgc
      781 caccaggggt ggacgatcga catgatggaa acggcgcggc cgggcgatgg caccgccctc
      841 gccagcgccg cggcacgtgc cggtgcccgt tgcgtggttg ccgtgggggg cgacggcgcc
      901 gtccacgaag tggccaacgg tctgctcgcc gtccgctccc aggccgtgct cggcgtcgtg
      961 ccgatcggct ccggcaatga cttcgcgaaa ctcgtgggag tctaccggca tgacgtcgcc
     1021 cgcgccgtgg cccgactcac gagggccgtg gtgcgccgct tcgacgcggg tcgcgctctc
     1081 gatgaatact tcgtcaacac gctcggattc ggcttcgggg cggcggtcgt gcgcacgcgt
     1141 aacgccatgc cgggcctcaa gggattcctc tcctatctga ttcccatatt ccggaccttt
     1201 gcgaactttc gcccgccggt cttcgaggtc cgctcggcag gcttcgagga acagggcagg
     1261 atgatgatgg tggaggtctg caacgggacg accgcggggg gcgactaccg ctttgcgccc
     1321 gacgccgatc cggccgacgg cctgctggac gtctgcctga tccggcgcgt cggcttggtt
     1381 aggtttgtgc ggagcctgcc gcacgtgatg cgcggcacgc atgggagcct gaaggatatc
     1441 gtcgccctgt tccggagcgc gtcgctcacg atccgcgccc cggaggctcc gctggtgctt
     1501 catctcgacg gggaactgcg agagcccgga gtgcacgagt gcactgtgac ggtggagccg
     1561 aaatgcctga acgtgctggt ggcgaagtga agcggcggac aggcggtcag gcggtcagac
     1621 ggtcacgcct gcgccgggcc ggagtgcttt cgttcgtcct cctcgcgctg tccgcctgtc
     1681 cgcccgagcg cctgaccgcc caggtcccgg agtcactgcc ccagcctccg gcaccgcgcg
     1741 acacgagcca ccaggtcggt cccgtcggcg cgttgtggcg ttcactcgtc ctccccggat
     1801 gggggcaggc cgccacgggg cgcaaggtca ccggagcgct gttcaccgtc tgggagggga
     1861 cgacgatgtt catgacgctc aaggccagcc aggaggcgaa ctacctcagg gtatccggcg
     1921 cacccaacga gcagtccaag cgccaggagg tgcaggactg gctcgtcctg tggggattca
     1981 accacctgtt ctcgggcgcc gaagcgtacg tcgcggcgca cctgcgcgat ttccccaggg
     2041 acctgaagat ccgggcagtc ccgggcggcg tcggggtgcg gttctccctg ccataaggca
     2101 tgaacgcggc tccactgggc gtcttcgact ctggcatcgg cgggctcacc gtcgcgcacg
     2161 cgctgttcga gtgcctgccg cacgaatccg tcatctactt cggcgacacg gcgcgcgtcc
     2221 cctacggccc caagtcgccg gagacggtgc ggcgctacag cggggagatc ctcgacttct
     2281 tggtgcggcg cggcgtgaag atggtggtcg tcgcctgcaa cacgagcacc gcacacgcgc
     2341 tcgagtttct gcgcagccgc tcaccggtac cggtgcaggg ggtgatcgaa ccgggagcgc
     2401 gcgccgctgc ggccgcgacg cgcgccggag gcatcggcgt gatcgggact gccggcacca
     2461 tcgcgagcgg cgcctacgaa cgcgccatca gggccgtccg tcccgaggtg gaggtcgcgt
     2521 cccaggcatg cccgctcttc gtgccgctcg tcgaagaagg atggttcgac cacccggcca
     2581 ccgagctggt cgcgcgggag tacctgcagc cgctcaagga tcggcaggtg gacgtcctcg
     2641 tgctggggtg cacgcactac ccgctgctca agccgctgct ttcacgggtg atggggccgg
     2701 aggtgacgtt gatcgacagc gccgcggaga cggcccgaac ggttcggcag cagctcgaag
     2761 gacaggggct gctggcgccc cccgactcgc ggtcggcgca ccgcttcgtc gtctccgacg
     2821 atgaggcgct cttccgtcgc gtcggcgccc ggttcctcgg ggcgaagctg caggacgtgg
     2881 agctggcgcc gctctagact ccgaaactct tcagctccac ggtctccggc gtcaccacgg
     2941 cgtaccgccc gccgtccagg aacgcgccgg ggttgaggta ggtgcgccca tcggagagcc
     3001 gctcgaggcg ggcccggtgc gtgtgtgcca tgacgacgag cgacagctcc gggcgacgct
     3061 cgagcaccgc cttcgcgtag gacgcctgcg cagcagcggc gcggtcgagt accgcctcgt
     3121 ccttcgtcgc gtcggccaac gtcccggaga gcttgtcggc gatccagaag ccgatcgcgg
     3181 gatgcagggt gcggaacacc gtgatggtga gcggctggcg cagcagccag tgtgtgagcg
     3241 tcccgcccca gtgctgttcg gtaaggccat cgccgtggac cgcgagcgcg cgccgcccgg
     3301 cgaggtccac ctctgccgcg ccggaatggt aggcgatccc gaggtcctgc cggagaaaat
     3361 tcccgcccca acgatcgtga ttgcctccca ggaaggtcac cgggatcccg cgggtgacga
     3421 gttggccgag cttcatcgcc gtgacggcat ggcggcgcgg gaccacacgg ccgtactcga
     3481 accagaagtc gaagagatcc ccgacgacca gcagctcgtc gccgggctgg ggcacggtct
     3541 ccagaaaggc gtgcaatgcg gcctcgacgt tcgcgggaac ctggcccagg tgtgcgtcgg
     3601 ccagcacgta acagcgcgcg gcggggggag acacggcccc aagctaggaa ccgcggtccg
     3661 gggtcgcaag ccactgtccc atttgacttt tgctcacagg ccgcgcattc tccggcacga
     3721 tgaccgtaac gagcggcgag tccagaattc ggcgcccgca ccccagagcg cccggggtgt
     3781 cgggcggtgc cgaaccggcc ggccagccgc caccggccgg tctggagcgc aacccgggca
     3841 cgccgctcga tctggatctg atccgagcca tt
//
LOCUS       DBNGZW010000390         2661 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1128097, whole genome shotgun sequence.
ACCESSION   DBNGZW010000390 DBNGZW010000000
VERSION     DBNGZW010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1128097"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1019
                     /locus_tag="AC16JC_09225"
     CDS             <1..1019
                     /locus_tag="AC16JC_09225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164915.1"
                     /translation="LAEELAQRLRLEGAATAVIRAVESDLETPWNGVLGLARGGLLEA
                     GGVAGAAPGALAWFAARIAEWADRFPAARSGSGEAPARAFSEVLGAAAASQPVVLVVD
                     DAEYLDRDSLLALGLALRDLARCPLLVLCTISSAAARPELEELRAQLGGDVDGVVLRL
                     APLGTADIKQLARWAVPGFDEAALDRLTRRVSTDSAGLPMLVVELLSAVVAGLDLDKV
                     RGAWPEPLQTLDQSLPGDLPEGIIAAIRVGFRRLSAAAQLVLKAATVLEGPIAAARLR
                     RVTKLDPAKVVDALDELEWTRWLSADARGYAFVARVVRQVIKQDLMLKGEIQRLLDAD
                     AEDQ"
     gene            1048..2007
                     /locus_tag="AC16JC_09230"
     CDS             1048..2007
                     /locus_tag="AC16JC_09230"
                     /inference="COORDINATES: protein motif:HMM:NF048868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Vgb family protein"
                     /protein_id="HIR5164916.1"
                     /translation="MKRILALILATAFQGPAVVEYPLPRGGAFPHDPAVGKDGIVWYT
                     DQRNSYIGRLDPATGKVIDYPTPTPASGPHGIVVAPDGAVWYTGNAKGLIGRVDPATG
                     TIKEFQLPAEARDPHTPVYHEGKLWFTAQGANLYGWLDPASGRVKTFPLSTPRARPYG
                     LVGGPDGFLWMALLGTNKVVRINPADGAAREYPLPDSGARPRRLQVDARGIVWYTDFA
                     RGYLGRIDPATGRVREFASPGGSGSGPYGIALGTDGKIWYDESGTNTIVMFDPASERM
                     QSVSIPTAGAIVRNMTVDSTRGRVWLALSGTQRLGRIDLTPGP"
     gene            2393..>2661
                     /locus_tag="AC16JC_09235"
     CDS             2393..>2661
                     /locus_tag="AC16JC_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164917.1"
                     /translation="MATRASSSFIGEFGIRPPIASVSSPERFLAASVGVLGDFADFEA
                     YGEDLLTIRATAGYHFLSPQHYGLRMAARPAVVAPTSGGEAELFL"
BASE COUNT          359 a          911 c          982 g          409 t
ORIGIN      
        1 ggctcgccga ggagctcgcc cagcggctgc gattggaggg ggcggcgacc gcggtcattc
       61 gcgccgtgga gagcgatctc gagaccccgt ggaacggcgt gctcggcctc gcccgtggcg
      121 ggctgctcga ggcgggcggg gtcgcgggcg ccgcgccggg cgccctcgcg tggttcgcgg
      181 cgcggatcgc ggaatgggcc gatcggtttc ccgcggcgcg gagcgggagc ggcgaagcgc
      241 ccgcgcgcgc gttcagcgaa gtcctcggtg cggcggcggc gtcgcagccc gtggtgctcg
      301 tggtggacga cgccgagtat ctcgatcgcg actcactgct cgcgctgggt ctggcgttac
      361 gcgatctggc ccgctgtccg ctgctcgtcc tgtgcaccat cagcagtgcc gcggcaaggc
      421 ccgagctgga ggagctgcgc gcgcagctcg gcggtgacgt tgacggcgtc gtgctgcgcc
      481 tggcgccgct cggcaccgcg gacatcaaac aactggcgcg ctgggcggtg ccggggttcg
      541 acgaggcggc gctcgatcgc ctcacccgcc gggtgagcac cgactccgcc ggcctcccca
      601 tgctggtggt cgagctgctg agcgccgtgg tcgcgggatt ggacctggac aaggtccggg
      661 gggcgtggcc cgagccgctc cagacgctcg atcagtccct gcccggcgac ctgcccgagg
      721 ggatcatcgc ggcgatccgc gtgggcttcc ggcgcctgag cgccgcggcg cagctcgtcc
      781 tgaaggcggc caccgtcctc gaggggccca tcgcggcggc gcggctcagg cgcgtcacga
      841 agctcgatcc ggcgaaggtc gtggacgcgc tggacgagct cgaatggacc cggtggctct
      901 cggccgatgc ccgggggtac gcattcgtgg cccgcgtcgt gcggcaggtg atcaaacagg
      961 acctgatgct caagggggag attcagcgcc tgctggacgc cgacgccgag gaccagtagc
     1021 agcgttcaca gacggaggaa ccccgacatg aaacgcatcc tcgccctcat cctcgcgacc
     1081 gcgttccagg ggccggcggt cgtggagtac ccgctgccgc gcggcggcgc gttcccgcac
     1141 gatcccgcgg tgggcaagga cgggatcgtg tggtatacgg atcagcgcaa cagctatatc
     1201 gggcggctcg atccggccac ggggaaagtg atcgactatc ccacgcccac tcccgcctcc
     1261 ggaccgcacg gcatcgtcgt ggcgcccgac ggggcggtgt ggtacaccgg gaacgcgaaa
     1321 gggctgatcg ggcgcgtgga cccggcgacc ggtacgatca aggagttcca gctccccgcg
     1381 gaggcgcgcg atccgcacac gccggtctac cacgaaggga agctctggtt cacggcgcag
     1441 ggcgccaatc tctacgggtg gctggatccg gcgagcggcc gggtgaagac gttcccgctg
     1501 tcgacccctc gcgcccgccc ctacggcctg gtcggggggc cggacggttt cctgtggatg
     1561 gcgctgctcg ggaccaacaa ggtcgtccgg atcaatccgg ccgacggcgc ggcgcgcgag
     1621 tacccgcttc ccgattccgg cgcccggccg cgccgcctgc aggtggatgc caggggcatc
     1681 gtctggtaca ccgatttcgc gcgcggctat ctcgggcgga tcgatcccgc gacgggccgg
     1741 gtgcgcgagt tcgcgagccc cggaggaagc ggcagcggac cctacggcat cgcgctgggc
     1801 acggacggca agatctggta cgacgagtcc gggacgaaca ccatcgtgat gttcgatccc
     1861 gcgagcgagc gcatgcagag cgtgtcgatc cccaccgccg gagcgatcgt gcgcaacatg
     1921 accgtcgatt cgacgcgcgg ccgcgtgtgg ctggcgctca gcggcaccca gcggctgggc
     1981 cggatcgatc tgaccccggg gccctaaagc aagcgcttga cgtctgcttg atcggcgcgg
     2041 cccattctct catcgggcgg cgtccagcgg ggctccgccc gtcctccaac cggagcgtgc
     2101 ctcatgcaac gcagtctcct cctgctcccc ctggtgctgg gtgggctggc gcatccgtcc
     2161 cgggcgcagg tgttccagcc ctggggtgcg caagtcgacc ggagcgtgtc cctcgatctg
     2221 cttcggccgt cgttcgacgg gggcggcacc tcgacgctca ccacgatcaa ccagctcgga
     2281 ttccgctggt cggtcgggaa gatcgtgctg gtggcggagg ctccgttcgt caacgcgaag
     2341 gcggacggcg cctcgtccgg gggccctgct gattggcaat ccgtttctcg gcgtggccac
     2401 gcgcgcgagc tcatcgttca tcggcgagtt cgggatccgg ccgccgatcg ccagtgtgtc
     2461 gagccccgag cgtttcctcg ctgcgagcgt gggggtgctc ggcgacttcg ccgatttcga
     2521 ggcttacggc gaggacctgc tgacgatcag ggccacggcc gggtaccact tcctctcgcc
     2581 gcagcactac ggcctgcgca tggccgcccg acctgcggtc gtggccccaa ccagtggggg
     2641 cgaggcggag ttgtttctcg a
//
LOCUS       DBNGZW010000391         1794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_648595, whole genome shotgun sequence.
ACCESSION   DBNGZW010000391 DBNGZW010000000
VERSION     DBNGZW010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1794
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_648595"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(346..1293)
                     /locus_tag="AC16JC_09240"
     CDS             complement(346..1293)
                     /locus_tag="AC16JC_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011793369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HIR5164918.1"
                     /translation="MNIHKNARTTPRSRGQIVQRVLTEQEAPAAVAAAVGISERTVRK
                     WLARYVAEADGGLADRSCRPRRSPGATPPRLVSWVERLRRQRWTGAAIAQALQLSAST
                     VARLLRRQGLGRLRQLEPPVPVQRYQWARPGDLLHLDVKKLGRIGRVGHRIHGDRRSR
                     VRGIGWEYVHVAIDDASRLAYVEVLPDERGVTTTQFLWRARGWFRRQGIRVRRVLTDN
                     GSGYVCARFARLCHSAGLRHLRIRPYTPRTNGKAERFIQTLLREWAYRRPYPSSRHRT
                     EALPAWLYYYNWERGHGALHGRPPMSRLVAPNNLLAVQT"
BASE COUNT          291 a          614 c          606 g          283 t
ORIGIN      
        1 tcatgtcgac ggcctcctcc gccacgctct gcggcagcat aggagatgcc attcgtcacg
       61 atcggtgagc cggagccgct ttgccaagcc cgccatgctc cggatccggc acccagggat
      121 ttcttgcccg gtacaagatt ccggcctgcc ggccaagggc ggcacgctgc atcgatcgag
      181 gctcccgcgc gtctcacggg catgggagga ccgctgcatg ctctaccgcc tgttggccct
      241 ttgggatcgc tatcgagccc tgacgaccgg ggtggtcatc ctgggcgttc tgggcctcgg
      301 cgcatatctg ctggagcatc tcggggctcg gtgagccccg cgtggctagg tttgaactgc
      361 caggagattg ttcggggcca ccaagcgact catgggtggt cgaccgtgga gcgcgccatg
      421 gccgcgctcc caattgtagt agtacagcca ggcaggcagc gcctcagtac ggtggcgcga
      481 actcggatag gggcggcggt aggcccactc gcgcaacagg gtctggatga agcgctccgc
      541 cttgccattg gtccgcggcg tgtagggacg aatgcggagg tggcgcaggc cggcgctgtg
      601 gcagaggcga gcaaagcgtg cgcagacata gccggagccg ttgtccgtca gcacgcggcg
      661 cacgcggatg ccctgccggc gaaaccagcc ccgggcccgc cagaggaact gcgtcgtggt
      721 cacgccgcgc tcgtccggta gtacttcgac gtaggccagc cgggaggcat cgtcgatggc
      781 gacgtgcaca tactcccagc cgatgccgcg cacccggctc cggcgatcgc cgtggatgcg
      841 atggcccacg cgtccaatcc gccccaactt cttgacgtcg agatgcagca gatctccggg
      901 gcgggcccac tgatagcgct ggacggggac tggaggctcg agctgccgca ggcgccccag
      961 gccctggcgg cgcagcaacc gggctaccgt cgaggcgctg agctgcaggg cctgggcgat
     1021 cgccgcgcct gtccagcgct gccggcgcag acgctccacc cagctcacca gccgcggcgg
     1081 ggtggccccc ggactgcggc gcggccgaca cgagcgatcc gccaagcccc cgtcggcctc
     1141 ggcgacgtag cgcgccagcc acttccgcac ggtgcgctca ctgatgccga ccgccgcagc
     1201 cacggccgcc ggcgcctcct gctcagtcag gacccgctgc acgatctggc ctcgactccg
     1261 gggtgttgtc cgggcattct tatgaatgtt catccgggcc tcctgtcctg cggtgatggt
     1321 cggtccgcaa ccaccagatt cgcagcggag acccggatgg acaatctcct ggcagttcac
     1381 agctaggtgc cgacgcctcc cctggttgtt gacctcactg tcagtgccgg cgctgccggg
     1441 gaaccggaca acgccattgc cgctcccagc gcgctccaga caggggcacg gcaggcttcg
     1501 tgtccgtaag ccaccggcga gagagtcttg ccccgccgcg agcaacggac caactgctag
     1561 ccgatcgccg gggtcatccg agctcgaagc tctgcgaaag cgccacgcgg tcccggaaag
     1621 tctagaggcg gtcaatgcga gtgtgtgaga tcgaggaacg gattttgcga aacgaaccca
     1681 ccgagggatg ctgggtgtcc gacgccatgg ccgggagcca aggcgggccc cgcaatcagc
     1741 gggtccaata ttccatgatc ctcgggtatc gcacggaccc cttctagggc cgca
//
LOCUS       DBNGZW010000392         7840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_173801, whole genome shotgun sequence.
ACCESSION   DBNGZW010000392 DBNGZW010000000
VERSION     DBNGZW010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7840
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_173801"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1429
                     /locus_tag="AC16JC_09245"
     CDS             209..1429
                     /locus_tag="AC16JC_09245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011729373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5164919.1"
                     /translation="MVYEFGEFTLDTKRHQLRRGAEPRHLEPQVYAVLSHLLEHSDRL
                     VTSRELIEHVWGDRFVTPGTLNSRIKALRQALDDDGMTQRVIETVHGSGFRMRVAVRT
                     VADLHEASATTASSQGAAPARQQILFCRAHDGVRIAYAKSGTPDAPPLVKPGNWLTHL
                     EFDWNSPVWRHWLTEMGRGHTLIRYDARGCGLSDHDAADVSFESWVRDLGAVVDAEGL
                     DRFSLLGISQGCAVAIAYAARYPQRVDKLVLYGGYAVGRSLRSGSAQEELERTLLHDL
                     IRVGWGRDNPAFRQVFGSLFLPEGTAEQHQWFNDLARTMPMETALRVRQTSDVIDVRQ
                     EAAQVRAPALVLHTRGDAMVPFEIGRQLAAHIPGARFVPLESRNHVLLETERAWSQFL
                     EEVRAFLGSDVGAG"
     gene            complement(1485..3041)
                     /locus_tag="AC16JC_09250"
     CDS             complement(1485..3041)
                     /locus_tag="AC16JC_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR5164920.1"
                     /translation="MSAKQLPPRPDLDQLKRQAKRLLQSGDRARFRAIPAFASISDDA
                     LARAPLALHDAQSVIAREYGFDSWKALREHVEAVTLEFDAALGEFLEAATDGRHDRAE
                     RMLALHPGIANANLFTALVLGDASGVDARLARDPSLATKPGGPRGWEPVHYVCYTSMA
                     QDSPERAAGLAAIARRLIAAGVDPNTRFPWLHHNVRRPVLWGASRVVRSLPLVAVLLD
                     AGADPNDGVTLPLAASAGDIPVLDALRAHGADVDQAWATDGATSLYAILNWSRTPEGV
                     RWLLEHGADPNAVFAENGEAPLHVVARAWGVALAEAMVAHGADIERKRGDGRTPYAVA
                     ELNGNRAVADWLRAQGASPDLLEVDRLVAACSRGDRAAADALLANNPGLRDQLTDDHY
                     IAFHQAAERGDVRALEAMLACGFDPNRPDAGIGKTALHTAAMEGWPDAVQVLLAHGAS
                     VHVRDREFKGQPLIWAAEGSRQRREGRDFAAVGKLLLDAGSPVDWETSDEPAEGILEI
                     VAFWRGATLH"
     gene            3575..5011
                     /locus_tag="AC16JC_09255"
     CDS             3575..5011
                     /locus_tag="AC16JC_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164921.1"
                     /translation="MLAREYGFAGWLDLIAEVQKRLGKGLEWAVTQARRIIHENDVEH
                     LKQLLADYPALLSWRGEEGDGGLLGMATSSYGDSFDPFRERHFTRAACAELLIDAGAV
                     VVPSVCDGLIDSRARGLLQLFQRKNLIPRTLRFFAALGDLDEVRACFDKGGNDLATVN
                     DACMCACRWEHEAVASFLLDQAIALDPALGQHIDGGEGRAAFIRYLMAGSLTFTHAAP
                     TGPWQAFLMHQVVRAIHDDALEAFVNVLRRESWLLGDSCVGFQAGLIERATIRDRGAF
                     ISVLLDLDPALLHRRPPPESQAIGFALTYVKPHLVPLLTRIWPVPDDLPHAAGMGDLA
                     RVRRWFDAAGKPALGDPARHLPASGARARNDLQWGAPNVQQVLDTALAWAVLNSHFEV
                     ADFLLEHGADINTRWSSHEPASILHELAGLHENYAAMQFLIDRGIDMTIVDYRWGGTA
                     EGWARHAAKNETMAQWLAAAERRRAAGS"
     gene            5200..5616
                     /locus_tag="AC16JC_09260"
     CDS             5200..5616
                     /locus_tag="AC16JC_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164922.1"
                     /translation="MNEASRMAWLRIVLRALAVGYFVAFIPWITLILLNAPILAPGSS
                     LAPLLRFQPYNAHYESMLTAIHLVWAVMLWRAAADPARHLLFLDFTIWASVAHGLVML
                     VATPIQKGLVMTFIEGVPLFAIAAVVWWLRPRPAGR"
     gene            5760..6404
                     /locus_tag="AC16JC_09265"
     CDS             5760..6404
                     /locus_tag="AC16JC_09265"
                     /inference="COORDINATES: protein motif:HMM:NF017959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HIR5164923.1"
                     /translation="MAFLKVLLVCGIVAPLLYVAMLVFVPMGWEGYDSASRTISELSA
                     IGAPTRSVWVPLGIIYTLLVAAFGWGIWLSTRGNRRLRVVGGLLIACGVFGLFWPPMH
                     LREVLAAGGGTSSDTLHIVWTAGSNLLTLLAMGFAAAAFGKRFRLYSIATMVLLVAFG
                     VMTSMGAPGIQANLPTPWIGVWERLIIAVWMPWLVVLAVKLLRRPQIAAQRSLS"
     gene            6401..7618
                     /locus_tag="AC16JC_09270"
     CDS             6401..7618
                     /locus_tag="AC16JC_09270"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003936774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR5164924.1"
                     /translation="MKKTPRIDGLVAPGFEAVRAEFERNFTQRGEIGGAVAAYWRGEK
                     VVDLWGGRRTPDGDDPWNEDTMVVVMSTTKGLSAMTLALANARGWLDYEVPVARYWPE
                     FAQQGKGAVTVRQLLGHEAGLVLLDEQLTIAKLRDLDYMALLLARQKPAWPPGTRHGY
                     HTMTLGLYMQELIRRVDPAHRSLGRFFHDEIAVPLGLEFHIGLPREIPSARLARVKPL
                     SRWRGLLALRYTPIAVTMKMLRPGSLLRRSFMGLDADPNDRSYLDVEVPAGNGVGTAR
                     AIARAYAAFAEGGAELGLTPATFARITARPVIAGKLDEVLGVPSYFSLGFLRPGPTVS
                     FGSTERAFGSPGAGGSFGFADPDAHLGYAYVMNKLDFYLENDPREKALRDALYRAIGR
                     RGAGTRAVEMVGA"
BASE COUNT         1203 a         2646 c         2657 g         1334 t
ORIGIN      
        1 ccagggtcag aatcgcactc ccagagaaca gaatcctcgg cacgagtttc ctcgtgaaat
       61 cgagcatctt taccatgagt ccctccggtg cgatgattgg cgccgctcct tcctcggcgt
      121 cgccgccgta tgatcgcgag ggcggggtcc ccgatgcttg aactccccat ggagaattcg
      181 tggagccgcc atggagacga tctgaggaat ggtctacgag ttcggtgagt tcaccctgga
      241 cacgaagcgc caccagcttc gtcgtggcgc ggaaccgcga cacctggagc cgcaggtgta
      301 cgccgtgctc agccacctgt tggagcacag cgaccgcctc gtgacgtccc gggagctcat
      361 tgagcacgtg tggggtgacc ggttcgtgac tcccgggacg ctcaactcgc ggatcaaagc
      421 actgcgacag gcgctcgacg atgacgggat gacgcagcgc gtgatcgaga cggtgcacgg
      481 cagcgggttt cgcatgcgtg tcgccgtccg gacggtggcc gacctgcacg aggcgagtgc
      541 gacgacggcg tcgagccagg gcgccgcacc ggccaggcaa cagatcctgt tctgtcgcgc
      601 gcacgacgga gtacggatcg cgtacgcgaa gagtggcact cccgatgccc cgccgctggt
      661 gaagcccggc aactggctca cacatctcga attcgactgg aacagccccg tgtggcggca
      721 ctggttgacg gagatgggac ggggccacac gctcatccga tacgacgcgc gcggctgtgg
      781 tctgtcggat cacgacgcgg cggacgtgtc gttcgagtcg tgggtgcgcg atctgggcgc
      841 ggtcgtagac gcggagggcc tggaccgctt ctcgctgctg gggatctcgc aggggtgcgc
      901 ggtcgcgatc gcgtacgcgg cgcggtaccc ccagcgcgtg gacaagctcg tgctgtacgg
      961 cggctacgcg gtgggccgga gcctccgcag cgggtcagcg caggaggagc tggagcgcac
     1021 gctgctgcac gatctgattc gcgtcggctg ggggcgcgac aatccggcgt tcagacaggt
     1081 attcggctcg ctcttccttc ccgaggggac cgccgagcag caccagtggt tcaacgatct
     1141 tgccaggacg atgcccatgg agaccgcgtt gcgtgtccgg cagacgtcgg acgtgatcga
     1201 tgtccgccag gaagcggcgc aggtccgggc accggcgctg gtgctgcaca cgcgcggcga
     1261 tgccatggtg ccgttcgaga tcgggcggca gctcgcggcg cacatcccgg gggcgcggtt
     1321 cgtcccgttg gagagccgga atcatgtgct gttggagacg gagcgcgcgt ggtcacagtt
     1381 cctggaggag gtgcgcgcgt ttctcggctc ggacgtgggc gccggctaac ctggttggag
     1441 gtcgtcgggc acccacaaag caggtccttc gaggctcagc acgttcagtg cagggtagcg
     1501 ccgcgccaga acgcgacgat ctcgagtatc ccttccgccg gctcgtcgga agtctcccaa
     1561 tccaccggcg acccggcgtc cagcagcaac ttccccacgg cggcgaaatc tctcccctcc
     1621 cgccgctggc gcgatccctc cgccgcccag atcaacggct gacccttgaa ttcccggtcg
     1681 cgcacatgca ccgatgcgcc gtgcgcgagc aggacctgca ccgcgtcggg ccatccctcc
     1741 atcgcggccg tatgcagcgc ggtcttgccg atccccgcat cgggacggtt cggatcgaag
     1801 ccacacgcca gcatcgcctc gagcgctcgc acgtccccgc gctcggcggc ctgatgaaac
     1861 gcgatgtagt ggtcgtcggt gagctggtcg cgcagcccgg ggttgttggc gagcagcgcg
     1921 tccgccgccg cacgatcgcc gcgactgcaa gcggcgacga gccggtcgac ctccaggagg
     1981 tccggcgacg cgccctgcgc gcggagccag tccgccaccg cgcgattccc attcagctcg
     2041 gcgacagcgt acggtgtccg tccgtccccg cgcttgcgct cgatatcggc cccatgcgcg
     2101 accatcgcct cggccagcgc cacaccccac gcgcgcgcca ccacatgcag gggcgcctcc
     2161 ccgttctcgg cgaagacggc gttcgggtcg gcgccgtgct cgagcagcca ccgcacgccc
     2221 tcgggcgtgc gactccaatt gagaatcgcg tagagcgacg tcgccccgtc cgtggcccac
     2281 gcctgatcga cgtcggcacc gtgcgcgcgc aacgcgtcca gcaccgggat gtcgcccgcg
     2341 ctggcggcga gcgggagcgt gacgccgtca ttgggatcgg cccccgcatc gagcagcacc
     2401 gcgacgagcg gcagcgaccg cacaacgcgc gacgcgcccc acaacaccgg gcggcgcacg
     2461 ttgtgatgca gccacgggaa ccgcgtgttc ggatcgacgc cggcggcgat gagccgccgg
     2521 gcgatcgcgg cgagccccgc ggcacgctcg ggcgaatcct gggccatgga tgtgtagcac
     2581 acgtagtgga ccggctccca gccacgcggt ccgcccggct tggtggcgag cgacggatct
     2641 cgcgcgagcc gcgcatccac cccgctcgca tctcccagaa ccagcgcggt gaacagattc
     2701 gcgttcgcga ttcccgggtg caatgcgagc atccgctcgg cgcgatcgtg gcggccgtcc
     2761 gtcgccgctt cgaggaactc gcccaacgcg gcgtcgaact cgagcgtcac ggcttccacg
     2821 tgttcccgca gcgccttcca ggaatcgaat ccgtactcgc gggcgatcac cgactgcgcg
     2881 tcgtgcagcg cgagcggtgc gcgcgcgagc gcgtcgtccg agatggacgc gaatgcgggg
     2941 atcgcgcgga agcgtgcgcg gtcgcctgac tggagcagac gcttcgcttg gcgcttgagc
     3001 tggtcgaggt cagggcgggg ggggagctgc ttggcggaca taacgcctcc atgctgtatg
     3061 ccagcggtcc gcaagccggc cagcacggag acagccgtgc agagaactcg ttcgcagggt
     3121 ggactcagtc cttcgcgcgg acccgcatgc gccccaagcg caccggaagg atgccggccc
     3181 tcggtttcgc cgtcaaggct gccagccggg gggagctgat ggcggcggtt gggccgcatt
     3241 gacattgccg cgccggtcta gggatactgt ctgcagcgcg ctaacggggc gccaccgacc
     3301 cgcggcaagg gaagcatccc acctgtgatg gtctgagtcg tgtgttttgc accttctttc
     3361 agcctggaat cagcggggta caggcgattc atgaaagagg gtctccatga gcacgccgaa
     3421 gtctctccct gcccatccat ccctggaatc cctgcgcaag caggcgaagc gcctcgctcg
     3481 cgccatcacc gccggcgacg ccgacgcaat cgcacgggca cgggcgcagc tcccgaacgc
     3541 cgagctgccg ctgtcgcagc gtgatgccca actcgtgctg gcgcgcgaat atggcttcgc
     3601 agggtggctc gacctcatcg ccgaggtgca gaaacgcctc ggcaaaggtc tcgagtgggc
     3661 cgtaacgcag gcccgccgca tcattcatga gaacgatgtc gaacacctga agcaactctt
     3721 ggcggactac cccgcgttgc tctcctggcg aggggaggaa ggcgacggtg gactgttggg
     3781 aatggccacc agctcctatg gcgacagctt cgacccgttt cgagagcggc atttcacgcg
     3841 cgcggcatgc gccgagctgc tgatcgatgc cggcgcggtc gtggtgcctt ccgtgtgcga
     3901 cggcctcatc gattcccgcg cgagggggct gctgcagctg tttcaacgca agaacctgat
     3961 tccccgcacc ctcaggttct ttgcggccct gggtgatctc gacgaagttc gtgcctgctt
     4021 cgataagggc ggcaacgatc tggccaccgt caacgacgcc tgcatgtgcg cgtgccgctg
     4081 ggagcacgaa gccgtcgcgt cattcctgct ggaccaggcc atcgcgctcg acccggcatt
     4141 gggccaacac atcgacggcg gtgagggccg cgccgccttc atccgatacc tcatggcagg
     4201 cagcctcacc ttcacgcatg cggccccgac cgggccatgg caggcgttcc tcatgcatca
     4261 ggtcgtgcgc gccatccacg acgacgcgct ggaagcgttc gtcaacgtgc tgcgtcgtga
     4321 gtcctggctg ctgggcgact cctgcgtcgg ctttcaggcg ggattgatcg aacgggcaac
     4381 catacgagat cgtggcgcgt tcatcagcgt gctgctggat ctcgatcccg cgctgctgca
     4441 ccgccgtccg cccccggagt cgcaggcgat cgggttcgcc cttacttatg tgaagcccca
     4501 tctcgtccct ctgctcaccc gcatctggcc cgtgcctgac gatctgccgc atgccgcggg
     4561 catgggagat ctcgctcgcg tcaggcgctg gttcgacgca gccggcaagc cggcgttggg
     4621 cgatcccgcc cgtcatctcc cggccagcgg cgcacgcgcg cgcaacgatc ttcagtgggg
     4681 cgcgccgaac gtgcagcagg tgctcgacac cgcactcgct tgggcggtcc tgaacagcca
     4741 tttcgaggtc gccgacttcc tgctcgagca cggcgccgat atcaacaccc gctggagctc
     4801 ccacgagccc gcgagtatcc tgcacgagct ggctggtctg cacgagaact acgcagccat
     4861 gcagttcctc atcgatcgcg gcatcgacat gacgatcgtc gactatcgct ggggcggcac
     4921 ggctgagggt tgggcgcgtc acgccgcgaa gaacgaaaca atggcccagt ggctcgccgc
     4981 ggcagaacga cggcgagcgg cgggatcatg atgaaggagc ttgacccgtc acatgccgtc
     5041 gttcgcatct gacccttccc gagaacggat aacgaaggtt tccctcctcc ccccatcggc
     5101 ggattctcga ggcgttgcgg ctcgctcggt cgtcgagatc gtgactggcg ggcccgtgcc
     5161 gcgtggttag gttgccggtg tagtggctgg gaggtcgaca tgaatgaagc gtcgcggatg
     5221 gcctggctgc gtatcgtcct gagagccctc gccgtcgggt acttcgtagc gttcatcccg
     5281 tggatcacgc tcatcctcct gaacgctccc attctcgcac ccggcagcag tctggcgcca
     5341 ttgctcaggt tccagcccta caacgcgcac tacgagagca tgctgaccgc gatccatctc
     5401 gtatgggcgg tgatgctgtg gcgtgccgcg gccgatcctg caagacacct cctctttctc
     5461 gatttcacca tctgggcgag cgtcgctcac ggtctggtga tgctcgtcgc gacgccgata
     5521 cagaagggcc tcgtgatgac gttcatcgaa ggtgtccccc tgttcgccat agccgccgtg
     5581 gtgtggtggc tgcgaccgag acccgcgggg cgctgagtct cttcaccgtc gcctcacatt
     5641 cccgtcaccc cgattccgcc acccttcctc cggtattgca aggaggaacc atggccgagc
     5701 atggaatcgc gatggtaccg ggcaggaccg tgcgcccggc gccggtatcc gaaggtcgta
     5761 tggcattcct caaggtcctg ctcgtgtgcg gcatcgtcgc accgctgctc tatgtcgcca
     5821 tgctggtctt tgtgccgatg gggtgggaag gctacgactc cgcgtctcgg accatcagcg
     5881 agctgtccgc gatcggggcc ccgaccaggt cggtctgggt gccgctcggg atcatctaca
     5941 ccctgctcgt ggccgccttc ggatggggca tctggctgtc gacgcgcggg aatcgtcgcc
     6001 tgcgcgtcgt gggcggcctg ctgatcgcct gcggcgtgtt cggtctgttc tggccgccga
     6061 tgcacctgcg tgaggtcctg gcggcgggcg ggggcacgtc gagcgatacg ctgcacatcg
     6121 tctggacggc cgggtccaac ctcctcacgc tgctcgccat gggcttcgcc gcggcggcat
     6181 tcgggaagcg gttccgcctc tactcgattg cgaccatggt gctgctcgtc gcgttcggcg
     6241 tcatgacctc gatgggtgcc ccgggcatcc aagcgaacct gcctacgccg tggatcggtg
     6301 tgtgggagcg tctcatcatc gccgtgtgga tgccctggct cgtggtgttg gcggtcaagc
     6361 tcttacggcg tccgcagatc gcggcgcaga ggtcactctc atgaagaaga caccccggat
     6421 cgacgggctg gtggcccccg gtttcgaagc ggtgcgcgcc gagttcgagc gcaacttcac
     6481 gcaacggggc gagatcggcg gcgccgtcgc agcgtactgg cgcggcgaga aggtcgtgga
     6541 tctctggggc ggccgccgca cgcccgacgg cgacgatccc tggaacgaag acacgatggt
     6601 ggtggtcatg tccaccacga agggcctctc ggccatgacg ctggcgctgg cgaacgcgcg
     6661 cggctggttg gactacgagg tgccggtggc gcgctactgg cccgagttcg cgcagcaagg
     6721 caagggcgcc gtcaccgtgc gccagctcct cggccacgaa gcggggctcg tcctgctcga
     6781 cgagcagctg acgatcgcga agctgcgcga tctcgattac atggcactcc tgcttgcacg
     6841 ccagaagccc gcctggccgc cggggacgcg tcacggctac cacacgatga ccctcgggct
     6901 gtacatgcag gagctcatcc ggcgcgtgga cccggcgcac cgctcgctgg ggcgcttctt
     6961 ccacgacgag atcgccgtgc cgctgggact cgagttccat atcgggctcc cgcgggagat
     7021 cccgtccgca cggctcgcca gggtcaagcc gctgtcccgg tggcgtggac tgctcgcgtt
     7081 gcgctatacc cccatcgcgg tgaccatgaa gatgctccgt ccgggctcgt tgttgcgccg
     7141 ctcgttcatg ggcctcgacg cagacccgaa cgatcgcagc tacctcgacg tcgaggtgcc
     7201 cgcgggcaac ggcgtcggaa ccgcgcgcgc gatcgcgcgc gcctacgccg cgttcgccga
     7261 aggcggtgcc gagctcggcc tgacgccggc aacgttcgcc cgcattacgg cccggcccgt
     7321 gatcgcggga aagctggacg aggtgctggg ggtgccgagc tacttctcgc tcggtttcct
     7381 gcggcccggg cccacggtgt ccttcggctc gacggagcgc gccttcggct cacccggtgc
     7441 gggcggctcg ttcgggttcg ccgaccccga cgcgcacctg ggctacgcct acgtgatgaa
     7501 caagctggac ttctacctgg agaacgatcc gcgcgagaag gcgttgcgcg acgcgctgta
     7561 ccgcgcgatc gggcgtcgcg gtgccgggac ccgcgcggtt gagatggtag gcgcctgagt
     7621 tccgttgtgt gtcgtgcggc ggcccggcgc tgatccgggc gtcgcgcgaa gacgtagtcc
     7681 ggaacactcg atccgaccac ctgcgtgcga cggctcactt ccgacccttg ggtgggtggt
     7741 cctggagggc catgtaggtc acggtgtccc aggcgttgtg aataccgatg aacagcagga
     7801 cgacggtcgc cgcgccgacc atgtacagcg cgcgggtgga
//
LOCUS       DBNGZW010000393         2731 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_923029, whole genome shotgun sequence.
ACCESSION   DBNGZW010000393 DBNGZW010000000
VERSION     DBNGZW010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_923029"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..465
                     /locus_tag="AC16JC_09275"
     CDS             199..465
                     /locus_tag="AC16JC_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164925.1"
                     /translation="MSAKRKKRAAKQKRRTSVVARTKRGPIRVRLAAKATAEERAEAV
                     LYIETLEANQQIAHGKSLTPGTTHQVKTDSKGKKTLIRKRYSAI"
     gene            complement(477..1970)
                     /locus_tag="AC16JC_09280"
     CDS             complement(477..1970)
                     /locus_tag="AC16JC_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR5164926.1"
                     /translation="MPINFIPNDPGASGGPPMRRKTPRAERASTVAGFNYVTHGSPAP
                     LPLGDPQFLFWQSREAALAAVAAYEGLDGRRVTRWARSANRRRLDLRPNAGTDLNAYY
                     DGQSLSFFVYTTGTKTTWSGASTDVVAHETGHALLDQVRPDLWDSSYTETNAFHEAFG
                     DCMAILTAFEDSATRLLMRTKLRRQNFVEATAEDLSDGVLRALGASHPAAKPRHARNT
                     FKWALPSTLPASGPPHVLSSEVHSFARVFTGCFYDTILNILRDRIGASNTPTSAQLAA
                     AVRTAGKLLLRAAAEAPVTVRFFQSVGRAMVLADRAANNGANRMAVHDAFQKHNIALG
                     SAAMLAPVAALDGTVGRRGALSRSAVQDLRTRLGATPAERMQVERREIGGLAVVCATH
                     LKQVPLGGLDRRLRGVVAVAPRPVLVRSVDRTAALLGGVPEATTSDDEVKAFVETLLS
                     SDRIAFDRPARSGIKSAARQDVRLRLPTHAVHKVGATKVLRRIRFAC"
     gene            complement(1993..>2731)
                     /locus_tag="AC16JC_09285"
     CDS             complement(1993..>2731)
                     /locus_tag="AC16JC_09285"
                     /inference="COORDINATES: protein motif:HMM:NF051532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="golvesin C-terminal-like domain-containing
                     protein"
                     /protein_id="HIR5164927.1"
                     /translation="RAWHIAALYDCALNRGHDCRLNGVQSNDFTVGVEHAGFVSQDSY
                     PTAQLETSAALVCGVTRRFDIPRDWQHIVGHGELQPQNRTDPGPRWPWVRYVHRIQAD
                     CGEAVGDDDDALNDSAVARADVPGGWTRSGETPGYYGGGYRWALTSPQASDAAVFSFR
                     LDSARTRTVEARWTAGSNRSSRVTYAVITGAGDTLGVVARDQRSNGGDWQTLGRWTFP
                     AGWSRVALLRRASGGGVVVADAVRVRE"
BASE COUNT          440 a          978 c          922 g          391 t
ORIGIN      
        1 cctacatcga gggctggtac aatcggcggc gccggcactc gacgctggac tacctgagcc
       61 ccgccgagta tgaactgcgg ctccgtgccg cctaactcag gtgtccatca aaccggggca
      121 acatcagggt gaggacacga aggccggagt ggagctacat tgggggagcc gggtggaccg
      181 tcacgcgaag gacccctgat gagcgcgaag cggaagaaaa gggcagcgaa gcagaagcgg
      241 aggacgagcg tcgtcgcccg caccaagcgc gggccgatcc gggtacgcct tgccgcgaaa
      301 gcgaccgcgg aggagcgggc cgaagcggtg ctttacatcg aaaccctgga agccaaccag
      361 cagatcgcgc acggcaagtc gctcaccccg ggcacgacgc accaggtcaa gaccgactcc
      421 aagggcaaga agaccctgat ccgcaagcgc tactccgcca tctagccgct cgctcgtcag
      481 caggcgaagc gaatccgtcg cagcaccttc gtcgcaccaa ccttgtgaac cgcatgggtc
      541 ggcagccgca ggcgaacgtc ctgcctggcc gcggatttga tccccgagcg tgccggccga
      601 tcgaacgcga tccgatcgct gctgagcagc gtctccacga acgctttcac ctcgtcgtca
      661 ctggtcgtgg cttccggcac tcctcccagc agcgccgcgg ttcggtcgac ggatcgcacc
      721 agcaccggcc gtggtgcgac cgcgacgacg cccctgagcc ggcggtccaa cccgccgagc
      781 ggaacctgct tcaggtgcgt cgcgcacacc accgccagcc ccccgatctc gcgccgctcc
      841 acctgcatcc gttcggccgg tgtggctccg agtcgcgtgc gcagatcctg aaccgcggag
      901 cgcgacagcg ccccccgccg gccgacggtg ccgtccagcg ccgcgaccgg ggccagcatc
      961 gccgcgctgc ccagcgcgat gttgtgtttc tggaaggcgt cgtggacggc catccgattg
     1021 gcgccgttgt tcgcggcccg gtccgccagc accatcgccc ggcctaccga ctggaagaac
     1081 cgcacggtga cgggcgcctc cgccgccgcg cgcaacaaca gcttgccggc ggtgcggacg
     1141 gccgcggcga gctgggcgga ggtcggcgtg ttggacgcac cgatccggtc gcgcaggatg
     1201 ttcaggatgg tgtcgtagaa gcacccagtg aacacgcgcg cgaagctgtg gacctcgctc
     1261 gagagaacgt gcggcggccc cgacgcaggg agcgtgctcg gcagcgccca tttgaaggtg
     1321 ttacgggcgt ggcgcggctt cgccgcgggg tggctggccc ccagcgcccg cagcacgccg
     1381 tcggaaaggt cctcggcggt cgcctcgacg aagttctggc gccgcagctt cgtccgcatg
     1441 agcagccggg tggcggagtc ctcgaaggcg gtgaggatcg ccatgcagtc gccgaacgcc
     1501 tcgtggaagg cgttggtctc cgtgtaggag ctgtcccaca gatcggggcg cacctggtcg
     1561 agcaaggcat ggcccgtctc atgcgcgacg acgtcggtgc tcgcccccga ccaggtggtc
     1621 ttggtaccgg tcgtgtacac gaagaagctg aggctctggc cgtcatagta cgcgttgaga
     1681 tccgtgcccg cgttgggccg gagatcgagc ctgcggcggt tcgccgagcg ggcccagcgg
     1741 gtgactctcc tgccgtcgag cccctcgtac gccgccaccg cagcgagcgc cgcctcacgg
     1801 ctctgccaga acaggaactg gggatcgccc aaaggaagcg gggcgggcga cccgtgcgtc
     1861 acgtagttga atcccgcaac ggtggacgcc cgctcggcgc gcggcgtctt gcgccgcatg
     1921 ggcggcccgc cgctggcccc gggatcgttg gggatgaagt tgatgggcat gttcggttcc
     1981 tccggcttca ggtcattccc tcacccgcac cgcgtccgcc acgaccacac cccctcccga
     2041 agcgcggcgc aacagcgcca cccggctcca tcccgccgga aacgtccagc ggcccagggt
     2101 ttgccagtcg ccaccgttcg accgttgatc ccgcgcgacg acgcccagcg tgtcgcccgc
     2161 gccggtaatc acggcgtagg tcacacgcga cgagcgattc gaaccggcgg tccagcgggc
     2221 ctcgaccgtg cgggtgcgag ccgaatcgag gcggaacgag aacacggcgg cgtcgctcgc
     2281 ctgcgggctc gtgagcgccc agcgatagcc gcccccgtaa taccccggag tctccccgct
     2341 cctcgtccag ccgcccggca cgtccgcccg tgcgacggcg ctgtcgttca gcgcgtcgtc
     2401 gtcgtctccc accgcttccc cgcagtccgc ctgtatccgg tgcacgtacc ggacccaagg
     2461 ccagcgcgga ccgggatcgg tgcggttctg cggctgcagc tccccgtgac ccacgatgtg
     2521 ttgccagtcg cgcgggatgt cgaagcggcg ggtcacgccg cacaccagcg ccgccgacgt
     2581 ctcgagctgc gccgtcggat aggaatcctg cgaaacgaat cccgcgtgct cgacgcccac
     2641 cgtgaagtcg ttgctctgca cgccgttcag ccggcagtcg tggccgcggt tcagagcgca
     2701 gtcgtacagc gccgcgatat gccacgcgcg a
//
LOCUS       DBNGZW010000394         3283 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1606125, whole genome shotgun sequence.
ACCESSION   DBNGZW010000394 DBNGZW010000000
VERSION     DBNGZW010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3283)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3283)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3283
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1606125"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1122
                     /locus_tag="AC16JC_09290"
     CDS             166..1122
                     /locus_tag="AC16JC_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HIR5164928.1"
                     /translation="MKARLTAAALALAWSGVILAGCTERPTEPLAAGTPVADVLIMNG
                     VVLPIADAGADVQTYPYEPVDLAGSAKDDLSRDIVSYLWAIEWGPDGGELSNAEAQNP
                     SFTPYMTGDYVLSLTACVQGEFDLLCSAPDQVTVYVGNREPQVSIAAKPTTVTVGETV
                     VFDGSGSYDPDQQELTYSWDFQDGWISNNVSPSHAFAATGEYWVTLEVSDGWLTSTDQ
                     ILITVEEKPGPRDGIVSLISGIQSLVSSGQLSPDRGDGLLSKLNAALTSVDNGLMTDA
                     CKQLSAFQKQVAAGVKARKLDSGVGQSLIQGAEAVRTQIGCA"
     gene            1228..1689
                     /locus_tag="AC16JC_09295"
     CDS             1228..1689
                     /locus_tag="AC16JC_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164929.1"
                     /translation="MRWLPIALLVVGAVPCPAQESGRVHGSMFVELGGNAAFGGSMNF
                     EVLLAGPLGVRGGAGIDLFSSTPVYPIQVVVLSGAGRSKLELAAGVTIAHENPMYSGN
                     WHWDGTKTFASGFLGYRYQRPRGFMFRIGVVPLLWTNAHIPWVAIGLGTTF"
     regulatory      1791..1914
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            2010..3236
                     /locus_tag="AC16JC_09300"
     CDS             2010..3236
                     /locus_tag="AC16JC_09300"
                     /inference="COORDINATES: protein motif:HMM:NF051835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005085 - guanyl-nucleotide exchange
                     factor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HIR5164930.1"
                     /translation="MSIRLTSSALALAWSGAVLAGCSEPPTAPSSGAGNVGATQSPAA
                     LFVGGVAATRGGWSLVSAGFSHSCGITPGGDAFCWGSNEEGRLGDGTTTSSNVPVPVS
                     GQHHFVSVSAGGVMSCGVTASGAAFCWGDDGVFELGDSGAAGGFSTVPVPVSGGHHFL
                     SIVAGNSQACGVTVGGDAYCWGNNFSGGLGDGTTDNRSVPVLVAGDHHFRSVSPSTFH
                     TCGLTTGGDAYCWGANDFGALGDGTTTNSDVPVLVQGGHHFVQVAAGDLHSCGLTAGG
                     AAYCWGLNSDGQLGDGTTQGSSAPVLVSGGHRFRSVVVAVSRHTCAVAVGGAALCWGA
                     NGDGQLGLGTTTPSNVPAPVLGDQHFLSISTGTSHSCGVAAGGSAFCWGRNADGQLGN
                     GTTTTSSVPTGVLGPS"
BASE COUNT          507 a         1062 c         1142 g          572 t
ORIGIN      
        1 cactgcaatt ccgcattccg cattccgcat tccgcgttcc ccatctccca ttccgcattg
       61 ccccgagatt ttcgcaggaa tctcaccggc gattcagcgc cattccgcat aattccctcc
      121 ggcgacacac gtagttcggc gattctcgtt ttcgtggagg aaaatatgaa agcgcgctta
      181 accgcggcgg cgctggcgct cgcctggagc ggcgtcatcc tggccggctg cacggagcgg
      241 ccgaccgagc cgctggcggc cgggaccccc gtggcggacg tcctcattat gaatggagtg
      301 gttctgccga ttgccgacgc gggggctgat gtccagacgt acccctacga gcctgtggac
      361 ctggcagggt cagcgaagga cgacctgagc cgtgacatcg tttcgtacct ctgggccatc
      421 gagtggggtc cggacggcgg ggagctttcc aacgcggagg ctcagaaccc gtccttcaca
      481 ccctatatga cgggcgacta cgtcctgtcc ctgaccgcct gcgttcaggg tgaattcgat
      541 ctcctgtgca gtgcccccga ccaggtcacg gtgtatgtcg gcaatcggga gccccaggtc
      601 tcgattgcgg cgaagccgac gacggtgacg gtgggcgaga ccgtggtctt cgacggcagc
      661 gggtcgtacg accccgacca gcaggagctg acctactcct gggacttcca ggacggttgg
      721 atatcgaaca atgtgtcgcc gagtcatgcg tttgctgcga ccggcgagta ctgggtcact
      781 ctcgaggtct cggacgggtg gctgaccagc acagaccaga tcctcatcac ggtcgaggag
      841 aaacccggcc cgcgggatgg catcgtgtcc ctgatcagcg ggatccagtc gctcgtctcg
      901 agcggccagc tgagcccgga ccgcggggac gggctgttga gcaagctgaa cgccgccctc
      961 acgagcgtgg acaacggcct catgaccgat gcctgcaagc agctgagcgc gttccagaag
     1021 caggtggcgg ccggcgtcaa ggccaggaag ctggattcag gagtcgggca atccctgatc
     1081 cagggcgccg aggctgtgcg cacgcagatc gggtgcgcgt aggcgtgctg cgggcaggtc
     1141 ggctcggcga ggcgggcggc tccgagcgcc ttgatgagtt cttgacgact tcctcttagg
     1201 ctggggtgga ctcacgagga ggtcacgatg cgctggctgc cgatcgctct gctggtcgta
     1261 ggcgccgtgc cgtgcccggc acaggagagt ggccgggtcc acggcagtat gttcgtcgag
     1321 ctcggcggca acgccgcgtt cgggggctcg atgaacttcg aagtgcttct cgccgggccg
     1381 ctcggggtgc gtggcggcgc cgggattgat ctcttctcgt ccaccccggt gtacccgatc
     1441 caggtcgtcg tgctctcggg ggccggcaga tccaagctgg agctggccgc cggggtgacg
     1501 atcgcgcacg agaatccgat gtactccggc aactggcact gggacggcac caagacgttc
     1561 gcgagcggtt tcctggggta ccggtatcag cggccgcgcg ggttcatgtt tcggatcggc
     1621 gtcgtgccgc tgctctggac caatgcccac atcccctggg tcgcgatcgg ccttggcacg
     1681 acgttctaga agagtgagtg agcacggcgc aggcgtcgcc accgataggc atgcggactt
     1741 tcgaaggaat ctcaccgacg attcagggtc agcctgcaag tatttcccgc acacgacaag
     1801 ggcaaacccg gccaaagccg ggggcgcaaa gccacgggtc ctcgctctcc tccgttccgc
     1861 cgccgctgaa tccaggcgct cacgaaggac ccccaagatc gccgagctgc cgcaggcgca
     1921 gttcggcgat gctcgttcac ggggcggtcc ggtccgttct cgccggagtc cgagcatcag
     1981 ggccaggcac agggttccgg ggaggctgta tgagcatacg actgacatcc agtgcgctcg
     2041 ctctggcatg gagcggcgct gtcctcgccg gctgctcgga gccgccgacg gcaccgtctt
     2101 ccggcgctgg caacgtcggc gcgacccaat cgccggccgc actgttcgtc gggggagtcg
     2161 cagcgacgag gggcggctgg tccctggtga gtgcgggttt ctcgcacagc tgcgggatca
     2221 cccccggcgg cgatgccttc tgctggggct cgaatgagga gggccggctg ggcgacggca
     2281 cgaccaccag cagcaatgtg cccgtgcccg tgtccggtca acaccatttt gtctcggtca
     2341 gcgcgggtgg cgtcatgagc tgcggggtca ccgcttcggg agcggccttc tgctggggtg
     2401 acgacggggt gttcgagctg ggcgacagtg gcgcggccgg tggattcagc accgttccgg
     2461 taccggtttc cggcggccac catttcctct cgatcgtcgc gggcaacagc caggcctgcg
     2521 gggttacagt cggcggagac gcctactgct ggggcaacaa tttttccggc gggctgggcg
     2581 acggcacgac cgacaaccgc agcgtcccgg tgctcgtcgc cggtgatcac cacttccgct
     2641 ccgtcagccc gagcaccttt cacacctgcg ggctcacgac cggcggcgat gcctactgct
     2701 ggggtgcgaa cgatttcggc gcgctgggcg acggtacgac caccaacagc gacgtccccg
     2761 tcctggtgca gggcggccat cacttcgtgc aggtggcagc gggagacctg cacagctgcg
     2821 ggctcacggc cggtggcgcg gcctattgct ggggactgaa cagcgacgga cagctgggcg
     2881 atggtacgac gcaggggagc agcgctcccg tgctcgtttc cggcggccac cgcttccgct
     2941 cggtcgtggt ggcggtgagc cggcacacgt gcgcggtcgc cgtcggcggc gcggcgcttt
     3001 gctggggagc caatggcgac ggccagctcg gtctcggcac gacgaccccg agcaacgttc
     3061 ctgccccggt gctcggcgac cagcactttc tctcgatcag cacggggacg tcacatagct
     3121 gcggcgtggc tgccggcggc agcgccttct gctggggtcg caatgcggac ggccagctcg
     3181 gcaacggcac gaccacgacg agcagcgtgc cgaccggtgt cctgggcccg tcgtgatatg
     3241 gacctgcacc gccggcggtg ttgcaggcca ttgcgccgct gcc
//
LOCUS       DBNGZW010000395         5478 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_923160, whole genome shotgun sequence.
ACCESSION   DBNGZW010000395 DBNGZW010000000
VERSION     DBNGZW010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_923160"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1388)
                     /locus_tag="AC16JC_09305"
     CDS             complement(<1..1388)
                     /locus_tag="AC16JC_09305"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5164931.1"
                     /translation="METFLQDLRFAARSLARSPGLTLAAILTLGLGIGANSAAFGVVD
                     RLFFRPPAHVVDPDRVVRVNVTRTQPPFGTSTYSIATFPRYADLRDHARMLSSVAAYA
                     GGGFSLGRGTGAQRVTGELVSASFFPLLGVQPERGRFFTAEEDRKGGERVAVLSHEFW
                     QRQFAGDPDVLGKTLQLNAGVYTIVGVAPRGFTGVELSGPDVWVPMSVAAPESRWPQV
                     LECEGCWWLETIARLAPGVTAAQAASEGTALYRAHVGDGGRVESEVDSKATVSLGSVH
                     KALGPNADKSARLSVWIIAVCGAVLLIACANVANLLLARALQRRREIALRVALGAGRG
                     RLIRQLYAESALLAVLGLGAAILITLWAGPLLRAALLPDAATAASLDPRVFAFSAALA
                     VATALLAGFAPALHAGVPDLSAALKSGAREGGVARSATRTGLLVGQVALTFVLLTGAG
                     LFISSLHKVLGLR"
     gene            complement(1396..2103)
                     /locus_tag="AC16JC_09310"
     CDS             complement(1396..2103)
                     /locus_tag="AC16JC_09310"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5164932.1"
                     /translation="MPGNGASLIQLQGIKKVFYTDEVETHALSDVHLDIKQGEYVAIA
                     GPSGCGKTTLLSILGLLDTPTDGSYTLDGRPVANLTAADRARVRNRQIGFIFQAFNLI
                     GDLTVYENVELPLTYRGMSSAERKRRVTEALERVGMSHRVKHYPAQLSGGQQQRVAVA
                     RAVAGDPAILLADEPTGNLDSSNGESVMELLRELHRGGATICMVTHDPRYAAHADRTI
                     ELFDGRVVEERAGTPVA"
     gene            complement(2193..3437)
                     /locus_tag="AC16JC_09315"
     CDS             complement(2193..3437)
                     /locus_tag="AC16JC_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015353268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HIR5164933.1"
                     /translation="MDIPRAPIKNRRRLWQAGIGVGALIIITLVLSSLKPAAPSIESG
                     TPWIDSVRRGEMLREVRGPGTLVPEHIRYITALASARVERILSQPGALVQPGTQLLEL
                     SNPDVQIQALQAEQQLTDAQAQLVSLRTNLQNQRLTQEGVVATTRTEFEKAKREAMGA
                     DSLVTHGLVARNDAALAKDRALELETRYRVEQQRLELMTSTIDSQVAVQASQVQRLRA
                     IAAFQQNRVRSLQVRAGDPGIVTDLSLQPGQWVTEGTILAKVIQPGKLKAVLQIPETQ
                     AKDVTIGQPAAIDTRNGIIKGHVSRTDANAVNGTVTIDVALDGALPSGARADLSVDGT
                     IEVERLHNVLYTGRPAYGQPNSTIGMFKLVEGGHYAVRVQVQVGRSSVNAIEVVRGLE
                     AGDKVILSDMSQYDNVDRVRIK"
     gene            complement(3704..4873)
                     /locus_tag="AC16JC_09320"
     CDS             complement(3704..4873)
                     /locus_tag="AC16JC_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164934.1"
                     /translation="MRNLVPLFVLALFAGPVAGQNPSSGQTPPTARRSVEFSGFVLLN
                     GFYTSARVNNSDVPQRAEATATDAGGVGGTIRQTRLGIFVSDPDVLGGSFSGEVDADF
                     YGGQQPSSGGRTFPLLRVRRALGTLAWSHAQLLFGQESPLVAERSPRSLASVGFPDFA
                     GAGNLWLWTPQGRITLEQRGTVRLALQAAILAPIAGTAQTAFDTQLDSAERAKRPALE
                     GRVHIGWGPSDDPSEIALGGHVGWIKGHDSTSADSILVSKAVTADLRLVFGVVEILGE
                     AYAGQALAGLGGGGIGQSLGARGVPVRTKGGWGQMNVRPVPHLMFGGGCGIDDPKDAD
                     LPATGRLRNLVCEGHAEWRPPGPLVFGLEYRRLETRYASGDFTANHVNLAAGYRF"
     gene            4960..>5478
                     /locus_tag="AC16JC_09325"
     CDS             4960..>5478
                     /locus_tag="AC16JC_09325"
                     /inference="COORDINATES: protein motif:HMM:NF012355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5164935.1"
                     /translation="MTAGALPAAAQGAPVRGRIVILEKDNKPSTDLGDAVVFLEGLGD
                     PSPAARFDVTISDKAFAPHVLVVPVGSTVAFPNHDPFDHNVFSVSEGNSFDLGLYGRG
                     EGKSVTLQHPGLVRVFCNVHPRMVALVLVMGTHHYAQPGADGSFTINGVGPGSYRLHV
                     WHERAPTEVVKEV"
BASE COUNT          826 a         1896 c         1918 g          838 t
ORIGIN      
        1 cggaggccga gcaccttgtg caggctgctg atgaacagtc ccgcgccggt caggagcacg
       61 aacgtgagcg ccacctggcc caccaggagt ccggtgcggg tcgcggagcg ggcaacgccc
      121 ccttcgcggg cgcccgattt cagcgccgcc gacagatcgg gtacgccggc gtggagcgcg
      181 ggcgcgaaac cggcgaggag cgcggtggcc acggccaggg cggcgctgaa cgcgaagacg
      241 cgggggtcga gcgaagcggc ggtcgcggcg tcggggagca gggcggcgcg cagcagcggg
      301 ccggcccaca gcgtgatcag aatcgcagcg ccgagtccca gcacggcgag gagagcgctt
      361 tcggcgtaca gctgccggat cagtcggcca cggccggccc cgagggccac gcgcagggcg
      421 atttcccggc gccgctggag cgcacgcgcc aggagcaggt tcgccacgtt ggcgcaggcg
      481 atcaggagca cagcgccgca gaccgcgatg atccatacgg agagcctcgc agacttgtcc
      541 gcgttgggac cgagtgcttt gtgcaccgag cccagggaga cggtggcctt ggagtcgacc
      601 tcggattcga cgcgaccgcc gtcgcccaca tgcgcgcggt acagcgcggt tccctcgctc
      661 gccgcctgcg cggcggtgac gccgggggcg agccgggcga ttgtctccag ccaccagcag
      721 ccttcgcact ccagcacctg tggccaccgg ctctcgggag ccgcgacgct catcggcacc
      781 cacacgtcgg gaccgctcag ctccaccccg gtgaatcccc tgggcgcgac gccgacaatg
      841 gtgtacacgc cggcattgag ttgcagcgtc ttgccgagga catcggggtc cccggcgaac
      901 tggcgctgcc agaactcgtg gctcaggacc gcgacgcgct ccccgccctt gcggtcctcc
      961 tcggccgtga aaaaccgccc gcgctcgggc tgtaccccca acagtggaaa gaacgatgcg
     1021 gaaacgagct ctcccgtgac tcgctgggcg ccggtgccgc ggcccaggct gaacccgccc
     1081 cccgcatacg ccgcgaccga cgacagcatg cgggcgtggt cgcggagatc cgcgtagcgc
     1141 gggaacgtgg cgatgctgta ggtggacgtg ccgaacggcg gctgcgtcct ggtgacgttg
     1201 acccgcacga cgcggtcggg atcgaccacg tgcgccgggg gccggaagaa cagacgatcg
     1261 accacgccga acgccgcgct gttggcgccg atgccgagcc ccagcgtgag gatggccgcg
     1321 agcgtgagcc cggggctcct ggcgagcgag cgggcggcga agcggagatc ttggaggaag
     1381 gtttccacga tgcgctcagg cgaccggtgt gccggcgcgc tcctccacca cgcgcccgtc
     1441 gaacagctcg atcgtgcgat cggcgtgggc ggcgtaccgg gggtcgtgcg tcaccatgca
     1501 gatcgtggcg ccgccgcggt gcagctcgcg caacagctcc atcaccgatt cgccgttcga
     1561 ggagtcgaga ttcccggtcg gctcgtcggc gagcaggatc gcggggtccc cggccaccgc
     1621 gcgggccacg gcgacgcgct gctgctggcc gccggagagc tgcgcggggt agtgcttcac
     1681 ccggtgcgac atccccaccc gctccagcgc ctcggtgacc cggcgcttgc gctcggccga
     1741 ggacatgccg cggtaggtga gcggcagctc gacgttctcg tacacggtga gatcgccgat
     1801 caggttgaac gcctggaaga tgaacccgat ctgccggttg cggacgcgcg cccggtcggc
     1861 cgccgtcagg ttggcgacgg ggcgcccgtc cagcgtgtag ctgccgtcgg tgggcgtgtc
     1921 gagcaacccg aggatcgaga gcagggtcgt cttgccgcac cccgagggcc ccgcgatggc
     1981 cacgtactcg ccctgtttga tgtcgaggtg cacgtccgag agcgcatgcg tctcgacctc
     2041 gtccgtgtag aagactttct tgatcccctg cagctgaatg agcgacgcac cattccccgg
     2101 cattggctct ccttgtgagg aggtgtcggg ttccaggggt cgggtgtcag ggccccgcac
     2161 catcctgaca cctggcaccc ggcacctgtc acctatttga ttctcacccg atccacgttg
     2221 tcgtactgcg acatgtccga cagaatcacc ttgtcgcccg cctcgagtcc gcggacgacc
     2281 tcgatggcgt tcaccgagct gcggcccacc tgcacctgca cccgcacggc gtagtggccg
     2341 ccctcgacca gcttgaacat cccgatggtg ctgttcggct gcccgtaggc cggacgcccg
     2401 gtgtagagca cgttgtggag ccgctcgacc tcgatcgtgc cgtccaccga cagatcggcc
     2461 cgtgcgccgc tggggagtgc gccgtcgagt gccacgtcga tggtcacggt tccgttgacg
     2521 gcgttggcgt cggtccggct cacgtgtccc ttgatgatgc cgttgcgcgt gtcgattgcc
     2581 gcgggctgcc cgatggtgac gtccttcgcc tgcgtttcgg ggatctgcag caccgccttc
     2641 agcttcccgg gctggatcac cttggcgagg atggtgccct cggtgaccca ctgcccgggt
     2701 tgcagggaga gatcggtgac gatgccagga tcacccgcgc gcacctggag cgagcggacg
     2761 cggttctgct gaaacgcggc gatcgcgcgc aaccgctgga cctgggaagc ctgaaccgcg
     2821 acctgggagt cgatcgtcga cgtcatgagt tcgagtcgct gctgctcgac gcgataccgg
     2881 gtctcgagct cgagggcccg gtccttggcc agcgccgcgt cgttgcgcgc caccaggccg
     2941 tgcgtcacga gtgagtcggc gcccatcgcc tcgcgcttgg ccttctcgaa ttcggtgcgg
     3001 gtcgtggcga ccacgccttc ctgcgtcagc cgctgattct ggagattggt ccgcaggctc
     3061 acgagctgcg cctgcgcgtc ggtgagctgc tgctcggcct ggagcgcctg gatctgcacg
     3121 tcggggttgg acagctcgag cagctgcgtc cccggctgca ccagcgcgcc gggctgcgac
     3181 aggatgcgct ccacccgcgc cgaggcgagg gcggtgatgt agcggatgtg ctccggcacg
     3241 agcgtgcccg ggccgcgtac ctcgcgcagc atctccccgc gccgcaccga gtcgatccac
     3301 ggcgtgccgc tctcgatgga aggcgcggcc ggcttgaggc tcgacagcac cagggtgatg
     3361 atgatcagcg caccgacgcc gatcccggct tgccacagcc ggcggcgatt cttgatgggg
     3421 gcgcgcggga tgtccaaaga cggtcctcta tcggttctct agggagtgga atgcgtcgac
     3481 ggcaccctcc cgagaaagca cggcacatgc caggggcgcc gtgggcaacc gatttgcgga
     3541 ctgtgggtta cgaagcgccg cgcgagagct cgtgccgcag gatgcgcgga cgtgggattg
     3601 cgcgtcccgg aatcgcacag gtgggacagg taccagggag ccaggtgtcg ggtgccaggg
     3661 ctcaacccag aaccctgaca cccgacacct gatgcctagc acctcagaac cggtaccccg
     3721 ccgctagatt gacgtggttg gcggtgaagt cgccgctcgc gtacctcgtc tccagtcgcc
     3781 ggtactcgag cccgaatacc aacggaccgg gtggccgcca ctccgcgtgc ccctcgcata
     3841 ccaagtttct gagcctaccg gtcgccggca ggtccgcatc cttcgggtcg tcgatgccgc
     3901 acccgccgcc gaacatgagg tggggcacgg gacggacatt catctgtccc cagccgccct
     3961 tggtgcgtac cgggacgccg cgtgcgccga ggctctggcc gatgccgccg ccgccgagtc
     4021 ccgcgagcgc ctgacccgcg tacgcctcgc ccagaatctc cacgacgccg aacacgaggc
     4081 ggaggtccgc ggtgacggcc ttcgatacga gaatcgaatc ggcgctcgtg gaatcgtgcc
     4141 ccttgatcca gcccacatgg ccgcccaggg cgatctcgct cgggtcgtcg ctcggccccc
     4201 agccgatgtg tactcgtccc tcgagggcgg gacgcttggc acgctcggcg gagtcgagct
     4261 gggtatcgaa cgccgtctgc gccgttccgg cgatgggcgc gagaatcgcc gcctggaggg
     4321 ccaggcgcac cgtgccgcgt tgctccagcg tgatccgccc ctgcggcgtc cagagccaga
     4381 ggttgccggc gcccgcgaaa tccggaaacc cgaccgaggc gagcgaacgc gggctccgct
     4441 cggccacgag cggcgactct tgcccgaaca gcagctgcgc gtgcgaccac gccagcgtgc
     4501 ccagtgcgcg ccgtacgcgc aacaggggga acgtgcgccc gcccgagctg ggctgctggc
     4561 cgccatagaa gtcggcgtcc acctcgccgg agaagctgcc ccccaggacg tcgggatcgc
     4621 tcacgaagat cccgagccgg gtctgacgga tcgtcccgcc cacgccgccc gcgtcggtcg
     4681 cggtcgcctc ggcgcgctgc gggacatcgc tgttgttcac gcgcgcgctc gtgtagaagc
     4741 cgttcaggag gacgaacccg gaaaactcca cgctgcgtcg ggcggtcggc ggcgtctggc
     4801 cggacgacgg gttctgtccc gcgaccggcc cggcgaagag ggcgaggacg aagagcggca
     4861 cgaggttacg catgcaccgt gactcctggc gtgatcggat gggttagact aagctgaacg
     4921 cccgtggatg caagaaccgc agtcgggctc gtcctcgcca tgacggcggg ggcgcttccc
     4981 gccgccgccc agggcgcgcc ggtgcgcggc cgcatcgtca tcctcgaaaa ggacaacaag
     5041 ccgagcaccg atctgggcga tgccgtggtg ttcctcgagg ggctcggaga cccgtcgccg
     5101 gccgcccggt tcgacgtcac gatcagcgac aaggcgtttg ccccccacgt cctcgtcgtc
     5161 ccggtcggct ccacggtcgc ctttccgaat cacgatccat tcgaccataa cgtcttctcc
     5221 gtttcggaag gcaacagttt cgatctcggg ctctacggtc ggggcgaggg caagtcggtc
     5281 acgctgcagc acccgggact cgtccgggtg ttctgcaacg tccacccgcg catggtcgcg
     5341 ctggtgctgg tcatggggac ccaccactat gctcagcccg gcgccgacgg ctcgttcacg
     5401 atcaacgggg tggggccggg gtcctaccgg ctgcacgtgt ggcacgagcg cgcgccgacc
     5461 gaggtcgtca aggaggtc
//
LOCUS       DBNGZW010000396         3786 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1049013, whole genome shotgun sequence.
ACCESSION   DBNGZW010000396 DBNGZW010000000
VERSION     DBNGZW010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3786)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3786)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3786
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1049013"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="AC16JC_09330"
     CDS             complement(<1..930)
                     /locus_tag="AC16JC_09330"
                     /inference="COORDINATES: protein motif:HMM:NF053443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR5164936.1"
                     /translation="MRTSLVLALCTTALPTPARPPAVAPPPAPRGYYRSPAIHGETIV
                     FTAEGDLWTVSIQGGEARRLTTHAAEESHAAISPDGTTLAFSAGYEGPTEVYTMPLGG
                     GLPRRRTWEGAPATVVGWTSDGRILYATAGSSTLPSTQLATLDPRSGRRELLPLAEAS
                     DGTYDATARVWYFTRYAFQGSHTKRYQGGTAQNIWCYTAGSAEAVPLTADYAGTSKTP
                     LWWNGRIYFASDRDGTMNLWSMDPEGHDLRQLTRHRGWDVRSPALGDGRIVYQLGADL
                     HLFDIARGTDTTLDIRLGSDFDQERVRWVSNPMT"
     gene            1099..3237
                     /locus_tag="AC16JC_09335"
     CDS             1099..3237
                     /locus_tag="AC16JC_09335"
                     /inference="COORDINATES: protein motif:HMM:NF015649.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5164937.1"
                     /translation="MMAGPGLPSLDLICLGPPTARLGGREPPPDVLWRKHLGLLIYLA
                     LSPDRTRSRDHLLGMLWPEKAEPHARHSLNEAVRRLRAGLGTDRLRTRGDTITLDGTD
                     LQVDVLRFAALSGERPAEAVALLRGDFLEGFVVEDAPAFEEWAAQERTRWRARGEEVL
                     VTHGKAALSAGRLDEAVQAALQALALEPHSEPAARLRLRAMALRGDAAGALAWYHQFT
                     AQLLSEVGERPGQELEALADRIRTRSWHHAAAPPVEVSVPLVGDAALQSRAFRVIGEA
                     MLGVPRTLAIIAGQGLGKTRLVLECLNRAALDGALTVLATPLEQDNDAPWSTLRALAR
                     AGLAAAPGVAATAPEALAVLAAVVPELGGRVQRVPADQGEVAAALARLFEAVAEERPL
                     VVAVDDAHFADRVTLEGLGAAIPAVRKAPLAFVFSCLPEPERGPAALVRLRGEVGRRL
                     PGDSVRLEPLGQDDVLRLVTALAPWCTTAEMRDRLSRRVRFETWGSPFLAVTLLQALA
                     RASTMRGDVLEWPRRGSTIDSPLPISVPDLVRMAVVARVSVLDAPDLLLLRAASVSGS
                     ALDPEMLSRTAGLAPEDVEEGLVRLERAGLVVNDGRRYAFAAPLIAEVVSGECLTAGQ
                     RRSLRLRTAEALARREGMDARVLRVELLSQVGPDESVFADAVAVAEAALETGAVRSGR
                     RAIAAAERAAGDTGGGRERLEELRRRLDSV"
     gene            3464..>3786
                     /locus_tag="AC16JC_09340"
     CDS             3464..>3786
                     /locus_tag="AC16JC_09340"
                     /inference="COORDINATES: protein motif:HMM:NF014809.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II transmembrane
                     domain-containing protein"
                     /protein_id="HIR5164938.1"
                     /translation="MPRYRRHLIPLALLGLGLIAACGGPFPQSTLSPTSDFGGEIDRL
                     FTGIFWWAVGVFVVVEGLLLFSILRFRHREGAPAPVQHHGHTALEIAWTLAPALILVF
                     IAVPTV"
BASE COUNT          474 a         1311 c         1430 g          571 t
ORIGIN      
        1 cgtcatcgga ttggacaccc agcgcacccg ctcctggtcg aagtcggagc cgaggcggat
       61 gtcgagcgtc gtatcggtgc cacgggcgat atcgaacagg tggaggtcgg cgccgagctg
      121 gtacacgatc cgtccgtcgc ccagcgcggg cgaccggacg tcccagccgc gatgccgggt
      181 cagctggcgc aggtcgtgac cctcgggatc catggaccac aggttcatcg tgccgtcgcg
      241 gtcggaggcg aagtagatcc gcccgttcca ccacagcggg gtcttgctcg tgccggcgta
      301 gtcggccgtc agcggcacgg cctcggcgga tccggccgtg tagcaccaga tgttctgggc
      361 cgtgccgccc tggtagcgct tggtgtggct gccctggaat gcgtagcggg tgaagtacca
      421 cacgcgggcg gtcgcgtcgt aggtcccgtc gctggcctcg gccagcggca ggagctcgcg
      481 ccggccggaa cgcgggtcga gcgtggcgag ctgggtggac ggcagtgtgg aggacccggc
      541 cgtggcatac aggatccggc cgtcggacgt ccatccgacg acggtcgcgg gggcgccctc
      601 ccacgtgcgc cgccgcggca gcccgccgcc gagcggcatc gtgtacacct ccgtgggtcc
      661 ctcgtagccg gcggagaagg ccagcgtcgt gccgtcgggt gaaatcgccg catgcgactc
      721 ctcggccgcg tgggtggtga ggcggcgcgc ctcaccgccc tggatcgaga ccgtccagag
      781 atccccttcc gccgtaaaca cgatcgtctc gccgtggatc gcgggcgaac ggtagtagcc
      841 gcgcggagcg gggggaggcg cgacggccgg gggtcttgcc ggcgtgggga gggcggtggt
      901 gcacagcgcc agcacgaggg aggttcgcat gatcccgggc tccggcagga tgggtctcgc
      961 ggcttggggt tccgcgctca ggcgggcgag gccagagcgg tccctaagat acacgttgcg
     1021 ctagccgaag ccgcgtcgca cccgtattgt tttccctctg gcgcgctcat gctccatacg
     1081 tcccagacac cagcgaccat gatggccgga cccggtcttc cttcgctcga tctgatctgt
     1141 ctggggccgc ccaccgcccg gctcggcggc cgtgagccgc cgcccgacgt gctgtggcgc
     1201 aagcacctcg ggctgctcat ctacctggcg ctctcccccg atcgcacgag gtcgcgagat
     1261 catctgctgg gcatgctctg gcccgagaag gcggagccgc acgcgcggca ctccctgaac
     1321 gaggcggtgc ggcggctgcg cgccggactg ggaaccgacc gcctgcgcac gcgcggcgat
     1381 accatcacgc tggacggcac cgacctccag gtcgacgtcc tgcgcttcgc cgcgctgtcc
     1441 ggggagcgcc ccgcggaagc cgtcgcactc ctgcgcggcg atttcctcga gggcttcgtg
     1501 gtggaagacg ccccggcgtt cgaggagtgg gccgcgcagg aacgcacgcg gtggcgcgcc
     1561 cgcggcgagg aggtgctcgt cacgcacggc aaggccgcgc tgtcggccgg acggctggac
     1621 gaagccgtgc aggcggcgtt gcaggcgctc gccctcgagc cgcacagcga gccggccgcg
     1681 cggctgcgcc tccgggcgat ggcgctgcgg ggtgacgcgg cgggcgccct ggcctggtac
     1741 caccagttca ccgcgcagct cctgtcggaa gtgggcgagc gacccggtca ggagctcgag
     1801 gccctggcgg accgcatccg gacccgttcc tggcatcacg ctgcggcgcc gccggtggag
     1861 gtgtcggtgc cgctggtcgg tgatgccgcg ctgcaatccc gcgccttccg tgtgattggt
     1921 gaggccatgc tgggggtgcc gcgcacgctg gccatcatcg ccggacaggg actcggcaag
     1981 acccgcctcg tcctggaatg cctcaaccgg gcggcgttgg acggcgcgct gaccgtcctc
     2041 gcgacgccgc tcgagcagga caacgacgcg ccgtggagca ccttgcgcgc cctggcccgc
     2101 gccggactcg ccgcagcgcc cggcgttgcc gccaccgcgc cggaggcgct cgcggtcctg
     2161 gcggccgtcg tcccggagct cggcggccgg gtgcagcgcg tgcccgccga tcagggagag
     2221 gtcgccgcgg cgctggcgcg actgttcgag gcagtggcgg aggagcgccc cctcgtcgtg
     2281 gcggtggacg atgcccattt cgccgaccgg gtgaccctcg aggggctcgg ggccgcgatc
     2341 ccggcggttc gcaaggcccc gctggcgttc gtcttttcct gcctgccgga gccggagcgt
     2401 gggccggcgg cgctcgtgcg gctgcgcggc gaggtgggac ggcggctgcc cggcgacagc
     2461 gtgcgtctgg agccgctggg acaggacgac gtcctgcgac tcgtcacggc gctcgccccc
     2521 tggtgcacga ccgccgagat gcgcgaccgg ctctcccgtc gcgtgcggtt cgagacctgg
     2581 gggagcccat tcctcgccgt cacactgctg caggcgctgg cgcgggcgtc caccatgcgg
     2641 ggcgacgtgc tcgagtggcc ccgccgcgga tccaccatcg acagcccgct ccccatcagc
     2701 gtgcccgatc tggtgcggat ggcggtcgtg gcgcgggtgt cggtcctcga cgcaccggat
     2761 ctgctgctgc tgcgcgcggc gagtgtgagc ggatcggcgc tcgatcccga gatgctgagc
     2821 cggacggccg gtctcgcgcc ggaagatgtc gaggaggggc tggtccgact ggagcgcgcc
     2881 ggactcgtgg tgaacgatgg gcgtcgctac gcgtttgccg ctcccctcat cgcagaagtc
     2941 gtctcggggg agtgcctcac ggcgggccag cggcgcagct tgcgtctccg tacggcggag
     3001 gcgctcgccc ggcgggaagg catggacgcc agggtgctcc gggtcgagct gctgtcccag
     3061 gtcggtcccg acgagtcggt cttcgcggac gcggtcgcgg tcgccgaggc ggcgctcgag
     3121 acgggcgcgg tgcgcagcgg gcgtcgtgcg atcgcggcgg cggagcgggc ggccggcgac
     3181 accggcggcg gtcgagagcg cctcgaggaa ttgcgccggc gcctcgacag cgtctaggcg
     3241 aaacggggtg ggggaccgga ccgcatcccg ccaccccgct cgagcgcgcg agagggcgcg
     3301 cgcgtgtcgt gccgtaacct gaaggccgca gcgccaacct atgaaccgaa agtcacgcac
     3361 cggtcatctg cccgaccggt gcgatcaagt ggttgaaggg cggggggatg cgcattacct
     3421 tctggtgcct tttgaaccga cctcacgggg gacgctcgac gccgtgccgc gatatcgacg
     3481 acacctgatc ccgctcgccc tcctgggcct tgggctgatc gccgcctgcg gagggccctt
     3541 tccccagtca accctctctc ccacatcgga ttttggcggt gagatcgatc gcctgttcac
     3601 cggcatcttc tggtgggcgg tcggcgtgtt cgtggtggtc gagggcctgc tgctgttctc
     3661 gatcctgcgg ttccggcacc gggaaggggc gcccgcgccc gtgcagcacc acggccacac
     3721 ggcgctcgag atcgcctgga cgctggcgcc ggccctgatc ctcgtgttca tcgcggtgcc
     3781 gacggt
//
LOCUS       DBNGZW010000397         4111 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_855279, whole genome shotgun sequence.
ACCESSION   DBNGZW010000397 DBNGZW010000000
VERSION     DBNGZW010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_855279"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..181)
                     /locus_tag="AC16JC_09345"
     CDS             complement(<1..181)
                     /locus_tag="AC16JC_09345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164939.1"
                     /translation="MANPIGRYDALTQRLVGTILSTPGATPSELRRAVLERGEVPPPL
                     ASYVDKVALHAYTVTD"
     gene            complement(186..1874)
                     /gene="treF"
                     /locus_tag="AC16JC_09350"
     CDS             complement(186..1874)
                     /gene="treF"
                     /locus_tag="AC16JC_09350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004555 - alpha,alpha-trehalase activity
                     [Evidence IEA];
                     GO_process: GO:0005991 - trehalose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HIR5164940.1"
                     /translation="MDHLRVTALLALLGCTGPGQRPDRYEPEHDLGALYRNVQLAGIF
                     PDSKTFADARPRVAPADILARYDSARRVGGFDYRAFVERNFDLPPAVGEGFRTDRSQT
                     MEAHIRALWPVLTRPPDSADARSTLIPLPNAYVVPGGRFREVYYWDSYFTMLGLIESG
                     RTDLVGHMLDNFAHLIATVGHIPNGNRTYYLGRSQPPFFGAMVALYARATDTTQALRY
                     LDALEAEHAFWMAGAERLSPAQTFRRVVRQPDGGLLNRYWDDVPEPRPESFRQDYELA
                     QTLPATEREAFYRNVRATAESGWDFSSRWMRDPKDLRTLETTELIPVDLNSLLYEAER
                     TIAALRAFRGRAGDREVADRYSRAAEQRRQALLAIAYDPEGGFFYDVRWRTGERVADR
                     PTLAAAAPLYFGLATPEQGVRVAARLEREFLKPGGFVTTLIASGQQWDAPNGWPPLEW
                     LAIEGVRRYRRDDLADAARDRWLALNRRTYRATGKMMEKYDVVDLNQRAGGGEYPTQD
                     GFGWTNGVALALAAQQRTVLPTRPPRPRIHAPVRNREQAARGAHVASSVEPAYP"
     gene            complement(1856..3481)
                     /locus_tag="AC16JC_09355"
     CDS             complement(1856..3481)
                     /locus_tag="AC16JC_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020258361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HIR5164941.1"
                     /translation="MATFGVFDLLVIAAYFLVLLGISVWAARREKNVSADFFLASRDV
                     GWLAVGASLFASNIGSEHLVGLAGTGASSGLAVGHFEWLACLILLLLGWVFVPFYLRS
                     GVYTMPEFLERRYNPASRAYFTWVSIIGYVLTKISVTLFAGGVVMRAVTGLNFWTSAV
                     VLIVVTGLYTVTGGLRAVIFTEVAQAAVLIIGSATLAVLGVRAAGGWSGLEAKVPADF
                     FSMWKPTTDPNFPWTGIVFGAPILGVWYWCTDQHIVQRVLAARNVTEARRGTILAGFL
                     KILPVFIFVLPGIVAAALYADVRSGATDSAYPVLVTRLLPAGFKGLVLAGMLAALMSS
                     LASAFNSCSTLLTWDVYRKARPQASEARLVTVGRLTTVALVGVSLLWIPCMKYISSQL
                     YIYLQSVQAYIAPPIAACFLLGLFFRRLNGAGAITSLVTGFVLGALRLGLELANGPAK
                     TGLPDGIWAWIAEINFLHFAVLLFAISTTVLVTVSLLTPAPAPAQIAGITYGTTPPGA
                     PAAAPADRQGWRVNGGLSILLAALLGVLWIIFA"
     gene            complement(3495..>4111)
                     /locus_tag="AC16JC_09360"
     CDS             complement(3495..>4111)
                     /locus_tag="AC16JC_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164942.1"
                     /translation="EVEARVRARLEALPAAERTYWEQVLSRSGVPADTLRFPALALDS
                     AGRPIPVMSTDPAAWLLLENLGEAREAELLRPFLLPYPVGLLIDGVGPVAANDVYATP
                     AVWQMFERDLYHSPRVVWGREVNVLVSALARRLRGRPDSVAGVVLTRTLDAVTRSGLK
                     QAELWSYRLEDGALRPFRYGSSSDVQLWSLTDLAVQFLLARLSP"
BASE COUNT          705 a         1395 c         1450 g          561 t
ORIGIN      
        1 cgtccgtgac cgtgtaggcg tgcaaggcga ccttgtccac ataggaggca agcggaggag
       61 gcacctcccc ccgctccagg acggcgcggc gcagctcgct cggcgtggcg ccgggcgtgg
      121 acaggatcgt gcccacgagt cgctgcgtca gggcgtcgta ccggccgatc ggattcgcca
      181 cggctctaag gatacgccgg ctcgacactc gacgcgacgt gcgcgccgcg ggcggcctgc
      241 tcgcgattgc gcacgggcgc atggatccgc ggtctgggtg ggcgtgtcgg gaggacggtc
      301 cgctgctgtg cggcgagcgc cagggcgacc ccgttggtcc acccgaagcc gtcctgggtc
      361 gggtattctc caccaccggc ccgctggttc aggtcgacga cgtcgtactt ttccatcatc
      421 ttgccagtgg ctcgatacgt gcggcggttc aacgcgagcc agcggtcccg ggcggcatcc
      481 gccaggtcat cgcgccggta gcggcgtacg ccttcgatcg ccagccactc gaggggcggc
      541 cacccgttgg gcgcgtccca ctgttgccct gaggcgatga gggtcgtgac gaaccccccc
      601 ggcttcaaga actcccgttc gagacgcgcg gcgacgcgca cgccctgctc gggagtggcc
      661 agtccgaaat acagcggtgc ggcggctgcc agtgtcgggc ggtcggccac gcgctccccg
      721 gtgcgccagc gcacgtcgta gaagaacccg ccttccggat cgtaggcgat cgccagcagc
      781 gcctggcggc gctgctcggc ggcccgcgaa taccggtcgg cgacctctcg gtcgcccgcg
      841 cggccgcgaa acgcccgcag cgccgcgatc gtgcgctcgg cctcatagag gagactgttg
      901 agatcgaccg ggatcagctc cgtcgtctcc agcgtccgca aatccttcgg gtctcgcatc
      961 cagcgactcg agaagtccca accgctttcg gccgtggccc gcacgtttct atagaacgcc
     1021 tcgcgttccg tcgcgggaag tgtctgggcc agctcatagt cctggcggaa ggactccggt
     1081 ctgggctcgg gcacgtcgtc ccagtaccgg ttgagtaacc ccccgtcggg ctggcgtacc
     1141 acccggcgga aggtctgggc gggcgacaac cgctccgcac cagccatcca aaatgcgtgc
     1201 tccgcctcca gcgcatcgag gtagcgaagc gcctgcgtgg tatcggtggc tcgcgcgtag
     1261 agtgctacca tcgcaccgaa gaacgggggc tggctgcggc cgaggtaata cgtgcggttg
     1321 ccgttgggga tgtgaccgac cgtggcgatc aggtgggcga agttgtcgag catgtggccg
     1381 acgagatcgg tgcgcccgct ctcgatgagc ccgagcatgg tgaagtagga gtcccagtaa
     1441 tagacctcgc ggaagcggcc gcccgggaca acgtatgcgt tcgggagcgg gatcagcgtc
     1501 gagcgggcat ccgcgctgtc gggggggcgc gtcaagacgg gccagagggc gcgaatgtgt
     1561 gcttccatcg tctgcgatcg atccgtccgg aagccttcgc ccaccgccgg cggaaggtcg
     1621 aaattccgtt cgacgaacgc ccggtaatcg aacccgccga cgcgacgcgc agagtcgtaa
     1681 cgcgcgagga tgtcggccgg cgcgacgcgt ggccgcgcgt cggcgaacgt cttggagtcc
     1741 gggaagatcc ccgccagctg gacattccgg tacaacgcgc ccagatcgtg ttcgggctcg
     1801 tagcggtcgg ggcgctggcc cggcccggta cagccgagca gcgcgaggag cgccgttacg
     1861 cgaagatgat ccacaggact ccgagcagcg cggcgagcag gatggacagc cccccgttca
     1921 cccgccatcc ctgccggtcc gccggcgccg cggcgggcgc accgggaggc gtcgttccat
     1981 aggtaatccc ggcgatctgg gccggcgcgg gcgccggggt gagcaggctc accgtgacga
     2041 gcaccgtcgt gctgatggcg aacagcaaca ccgcgaaatg caggaagttg atctcggcga
     2101 tccacgccca gatgccatcg ggcagcccgg tcttcgccgg gccattggcc agctccaggc
     2161 ccagccgcaa ggcgccgagc acgaagccgg tcaccagcga ggtgatcgcc ccggcaccgt
     2221 tcagccgacg gaagaacagc ccgagcagga aacaggcggc gatcggcggg gcaatgtacg
     2281 cctgcacgct ctgcagatag atgtagagct gggacgagat gtacttcatg cacgggatcc
     2341 agaggaggct gaccccgacg agtgcgaccg tcgtcagccg ccccaccgtg acgagccggg
     2401 cctcgctggc ctgaggcctc gccttgcggt acacgtccca ggtgagaagg gtcgagcagg
     2461 aattgaacgc cgaggcgagg gagctcatca gggccgcgag catgccggcg aggacgagac
     2521 ccttgaagcc ggcgggcagc agccgcgtca ccagcacggg gtacgccgag tcggtggcgc
     2581 ccgagcgtac gtcggcgtac agggccgcgg cgacgatgcc gggcagcacg aagatgaaca
     2641 ccggcaggat cttcaggaac ccggccagga tcgtgccgcg gcgcgcctcg gtcacgttgc
     2701 gggcggcgag cacgcgctgc acgatgtgct ggtcggtgca ccagtaccag acgccgagga
     2761 tgggggcgcc gaacacgatg ccggtccacg ggaagttcgg atcggtggtc ggcttccaca
     2821 tggagaagaa gtccgcgggc accttggcct cgaggccgct ccacccgccg gcggcccgga
     2881 cgcccagcac ggccagcgtc gcggagccga tgatcaggac cgccgcctgc gccacttccg
     2941 tgaagatgac ggcacgcaga cctcccgtca cggtgtacag acccgtcacg acgatcagca
     3001 cgacggcgct cgtccagaag ttcagccccg tcaccgcgcg catcacgacg ccgccggcga
     3061 acaacgtgac gctgatcttg gtgagcacgt acccgatgat cgagacccat gtgaaatagg
     3121 cgcgcgacgc cgggttgtag cggcgctcca ggaactcggg catggtgtac acgccgctgc
     3181 gcagatagaa cggcacgaac acccacccga ggagcagcag gatcaggcac gcgagccact
     3241 cgaagtgccc cacggcgagc ccactcgatg cccccgtgcc ggcgagcccc acgaggtgct
     3301 cgctcccgat gttcgaagcg aagaggcttg cgccgacggc gagccagccg acatcgcggc
     3361 tcgcgaggaa gaaatccgcg ctcacgttct tttcccgccg tgcggcccag acactgatgc
     3421 cgagcaggac caggaagtaa gcggcgatga cgagcaggtc gaagacgccg aaggtggcca
     3481 cggtcagggt ccctctaggg ggaaagccgc gcgagcagga actgcacggc gaggtcggtg
     3541 agactccaca gctgcacgtc ggagctcgac ccgtaacgga acggccggag ggcgccgtcc
     3601 tcgagccgat agctccacag ctcggcctgc ttgagccccg agcgggtcac cgcgtcgagc
     3661 gtccgggtga gaacgacgcc ggcgaccgag tcgggcctcc cgcggagtcg ccgcgcgagg
     3721 gcggagacca gcacgttcac ctcccggccc cacaccaccc ggggagaatg gtacaggtcc
     3781 cgctcgaaca tctgccacac cgcgggagtg gcgtagacgt cgttggcggc gacgggaccg
     3841 acgccgtcga tcaacaggcc caccgggtag ggcagcagga acgggcgcag caactcggct
     3901 tctcgggcct cgcccaggtt ctcgagcagc agccacgccg cggggtcggt gctcatcacc
     3961 ggaatcggcc ggcctgcgct gtcgagcgcg agggcgggga atcgcagcgt gtccgccgga
     4021 accccgcttc tgctcaggac ctgctcccag tacgtccgct cggcggcggg gagcgcctcg
     4081 agccgggcgc gcacccgcgc ctccacctcg g
//
LOCUS       DBNGZW010000398         5436 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1198362, whole genome shotgun sequence.
ACCESSION   DBNGZW010000398 DBNGZW010000000
VERSION     DBNGZW010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5436)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1198362"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2158)
                     /gene="priA"
                     /locus_tag="AC16JC_09365"
     CDS             complement(<1..2158)
                     /gene="priA"
                     /locus_tag="AC16JC_09365"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication restart helicase PriA"
                     /protein_id="HIR5164943.1"
                     /translation="MTRFADVLLPLPVSTPYRYAIPDALGGRAVPGARVIVPVRGRRA
                     LGIVTAESGEPPVQAARDLLDAPDQEPALSPALLRLGEWLSSYYGCPTGLALRALLPG
                     ALWGGAARRAGRDPAGRFERTITLVRALPSLVEREREFSRAPKRRVVYETLEALGGSA
                     PVRHLVERLKLGTTVLEGLVRQGLARIDRVAAPRDPFADLAVAPPPTLTPDQRTVVAP
                     ILATPADVPVLIEGVTGSGKTLVYLDVVRSVVAAGGGAILLVPEIALTPQTVARVRGV
                     FGDKVAVLHSGLSDAERADAWRALRAGTKRVAVGARSAVFAPVQRLGAIVVDEEHEPS
                     YKQGTAPRYHTRDVALVRARLEGARLILGSATPSLETLHRVSTGAVRRCSLPRRVDDR
                     PLPPVELVDLRSAARVPEAGSVPWSEALDRAVRDALDRREQVILLLNRRGFASFLQCP
                     SCGDVPGCPRCAIALTVHATPAALRCHYCGHEEAVRTECVRCGGPTRRMRGLGTQQLE
                     HFVGLRFPAARIARMDLDTTSTKWSHHRILERVARGEVDVLLGTKMIAKGLDFPRVTV
                     VGVVDADTGLHLPDFRAAERTFQLVAQVAGRAGRGPAGGQVIVQTRAPDHHALRAAAA
                     HSVEAFAAEELPLRTAPNPSYPPHVGLVRFVALGPVEEVAAVRAGRVADWLRRASADR
                     LGGALTVLGPAACPIPRLHERWRWHVLVKAAEPRALG"
     gene            complement(2155..3177)
                     /locus_tag="AC16JC_09370"
     CDS             complement(2155..3177)
                     /locus_tag="AC16JC_09370"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005041935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016855 - racemase and epimerase activity,
                     acting on amino acids and derivatives [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HIR5164944.1"
                     /translation="MALTLSAEVVTLHTKHPFVIARGGRSAYEVVWVRLTDGDGIEGW
                     GEASPSSYYGETAQTVLTVLDTLSEHLPDDPFDLEGAEQRFERTVSKNGAARAALSAA
                     LHDLVGKRFGQPLWRLWGLDPTRAPQSSFTIGIDTPERVRQKVAEAAEYPILKIKLGT
                     DHDEDILRTIRAETDKPIRVDANAGWSRAQALGMLPVLKSYGVELLEQPLVPDDLEGI
                     AAVRRASPGIPVVVDESCIVATDIPKVAGAVDGINIKLAKCGGLREALRMIATARAHG
                     LLVMVGCMIETSIGITAAAHFTPLVDVADLDGAALTADDPFTGASIARGRITLPTGPG
                     LGVARR"
     gene            complement(3168..4190)
                     /gene="corA"
                     /locus_tag="AC16JC_09375"
     CDS             complement(3168..4190)
                     /gene="corA"
                     /locus_tag="AC16JC_09375"
                     /inference="COORDINATES: protein motif:HMM:NF013694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HIR5164945.1"
                     /translation="MTLSPAAPAAAAPKHWPASLYRRPDGTLEQDLPPLRLREILERD
                     EGELWVDIDSTDVHQHALLEKIFGFHPLAIEDTMSPGSRVKLEEYDRYIFVVISGIRF
                     DRATPDPFDLETFKLYFFLGKNFLVTVHAVESPSCDITRDRLTRSPDLLGRGAEMTMH
                     SIIDQAVDAYFPIVESLNDQVDQLEERLFEAFDEKLIHEIFKAKRSAFGLRRHVGPLR
                     EVLNVLTNRPSIYIRSETQLYYRDVYDHTIRIMESVDTTRDLLAGVLETYLSQTSNRM
                     NRVMKQLSIVSTIALPLIVVGGIYGMNFSQMPFTHHPLGFYLALGSMAAISAVMLWWL
                     HRNRWL"
     gene            complement(4219..5130)
                     /locus_tag="AC16JC_09380"
     CDS             complement(4219..5130)
                     /locus_tag="AC16JC_09380"
                     /EC_number="3.5.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF013046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000118 - histone deacetylase complex
                     [Evidence IEA];
                     GO_function: GO:0004407 - histone deacetylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR5164946.1"
                     /translation="MPTTVTYHPATRLHPGRAGHPERPERIEAVLEALRAPALAPAVS
                     WAEATSADVTALELVHPRRYLNALEALAAAGGGPIDPDTYMGPRSWEAVTASAGVALR
                     AVTEAIRPRGGGTAFAATRPPGHHALASRAMGFCFVNNVVVAARHAQALGAARVLIVD
                     WDVHHGNGTQALVEQDASIRFVSMHQYPWYPGTGAADERGVGNVFNVPRPPGLPRATY
                     VHDLLEAVDAALVGWTPDVLLLSAGFDSLGGDPLGGFTLTPEDIATWTTALRTRLDGR
                     PVAAVLEGGYRLDLLVAGVTALVRALA"
     gene            complement(5135..>5436)
                     /locus_tag="AC16JC_09385"
     CDS             complement(5135..>5436)
                     /locus_tag="AC16JC_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164947.1"
                     /translation="HRAPGAAARGAVTTRGRPLATFAVGFLLLDAVLLAWAGLEQRRN
                     SLLAGAIACALAAAAVVLLWRRYRRTLVDLDAGRAEMKAEAEAIRRLLREKNLHN"
BASE COUNT          798 a         2020 c         1917 g          701 t
ORIGIN      
        1 gccccagcgc ccgcggctcg gccgccttca ccagcacgtg ccagcgccac cgctcgtgga
       61 ggcgcgggat gggacacgcc gcggggccaa gcaccgtcag cgctccaccc agccggtctg
      121 cactcgcccg ccgcagccag tcggccaccc ggcccgcccg cacggcggcc acttcctcga
      181 caggccctag cgccacgaac cggaccagtc ccacgtgcgg cggatacgag ggattgggcg
      241 cggtgcgcag cggcagctcc tcggccgcga acgcctccac cgaatgcgcc gccgcggccc
      301 gcaacgcatg gtggtccggt gcccgcgtct gcacgatcac ctgacccccg gccgggccgc
      361 gccccgcccg gcccgcgacc tgcgccacca gctggaacgt ccgctccgcc gcccggaagt
      421 cgggcaggtg cagtcccgtg tccgcatcca ccacgcccac caccgtgacc cgcgggaagt
      481 cgagcccctt ggcgatcatc ttggtgccga gcagcacgtc cacctcgccc cgggcgaccc
      541 gctcgagaat gcggtgatgg ctccacttgg tgctggtcgt gtcgaggtcc atgcgggcga
      601 tgcgcgcggc cgggaagcgc agccccacga agtgctcgag ctgctgcgtg ccgaggccgc
      661 gcatgcgccg ggtcggcccg ccgcagcgga cgcactcggt ccgcaccgcc tcctcgtgcc
      721 cgcagtagtg gcagcgcagc gcggcggggg tggcgtgcac ggtgagcgca atggcgcagc
      781 gcgggcatcc gggcacgtcg ccgcaggacg ggcactgaag gaacgaggcg aagccgcgcc
      841 ggttgagcag caggatcacc tgctcccggc ggtccagggc gtcgcgcacg gcccggtcga
      901 gggcttcgct ccacggcacc gatccggcct cgggaacgcg cgcggcgctg cgcaggtcca
      961 ccagctcgac gggcggcaac ggccggtcgt ccacccgccg cggcagactg cagcgccgta
     1021 ccgcgccggt cgacacccga tgcagcgtct cgagcgacgg cgtggcgctc cccaggatca
     1081 gacgggcccc ctcgagccgg gcccgcacca gcgcgacgtc ccgagtgtgg tagcgtggcg
     1141 cggtgccctg cttgtagctc ggctcgtgct cttcgtccac gacgatggcc ccgagccgct
     1201 gcaccggcgc gaacaccgcc gagcgcgccc cgacggccac ccgcttcgtg ccggcgcgca
     1261 gcgcgcgcca cgcgtccgcc cgctcggcgt cggagagccc ggaatgcagc accgccacct
     1321 tgtcgccgaa cacgccgcgc acccgcgcca ccgtctgcgg ggtgagggcg atctccggga
     1381 cgaggaggat ggcgccgccg cccgcggcga cgaccgaccg aacgacgtcg agatagacca
     1441 gcgtcttccc ggatccggtc acgccctcga tgagcaccgg cacgtcggcc ggcgtcgcga
     1501 ggatcggcgc caccacggtc cgctgatcgg gcgtcagcgt gggcggcggg gcgaccgcca
     1561 ggtcggcgaa cggatcgcgc ggcgccgcca cgcggtcgat ccgggccaac ccctgtcgca
     1621 ccagcccctc cagcaccgtg gtcccgagct tcaggcgctc caccagatga cgcaccggcg
     1681 ccgagccgcc cagggcctcc agtgtctcgt acacgacgcg tcgcttcggc gcgcgcgaga
     1741 actcgcgctc ccgctccacc agcgacggca gcgcgcggac cagggtgatc gtgcgctcga
     1801 agcgcccggc cggatcgcgg cccgcgcgcc tggcggcgcc gccccacaac gcgcccggca
     1861 gcagcgcccg cagcgccaga cccgtcgggc acccgtaata gctcgagagc cactcgccca
     1921 gccgcagcag ggccggcgac agcgccggct cctggtccgg ggcatccagc aggtcgcgcg
     1981 ccgcctggac gggcggctcg ccgctctcgg ccgtgacgat gcccagcgcg cgccgcccgc
     2041 gcaccggcac gatcacgcgg gcgcccggga cggcccggcc gccgagcgcg tcggggatgg
     2101 cgtagcggta gggtgtggag acggggagcg gcagcaggac gtcggcgaac cgcgtcatcg
     2161 ccgcgcgacg cccagccccg gtcccgtggg aagggtgatc cgcccgcgcg cgatcgacgc
     2221 gccggtgaac gggtcgtccg cggtgagcgc ggcgccgtcc aggtccgcca cgtccaccag
     2281 cggggtgaaa tgggcggccg cggtgatgcc gatcgacgtc tcgatcatgc accccaccat
     2341 gacgagcagc ccatgggcgc gcgcggtggc gatcatgcgc agcgcctccc gcagcccgcc
     2401 gcacttggcg agcttgatgt tgatcccgtc cacggcaccg gccactttcg ggatgtcggt
     2461 cgccacgata cagctctcgt ccaccacgac cgggatgccc ggggacgcgc gccgcacggc
     2521 cgcgatgccc tccaggtcgt cgggcacgag gggttgctcc aacagctcca ctccgtacga
     2581 cttcaacacg ggcagcatgc cgagggcctg cgcgcgcgac cagccggcat tggcgtccac
     2641 gcgaatcggc ttgtcggttt cggcgcggat cgtccgcaga atgtcttcgt cgtgatcggt
     2701 cccgagcttg atcttgagga tcggatactc cgcggcctcc gccaccttct ggcgcacccg
     2761 ctccggcgtg tcgatcccga tcgtgaacga cgactgcggg gcccgggtgg ggtcgaggcc
     2821 ccacaggcgc cacagcggct gcccgaagcg cttgccgacg agatcgtgca gcgccgccga
     2881 cagggcggca cgcgcggcgc cgttcttgga gaccgtgcgc tcgaagcgct gctccgcgcc
     2941 ttccagatcg aacgggtcgt cgggcaggtg ctccgacagc gtgtcgagca cggtgaggac
     3001 cgtctgggcc gtctccccgt agtacgacga cggcgacgcc tcgccccagc cctcgatccc
     3061 gtcgccgtcg gtgaggcgca cccacaccac ctcgtaggcg ctgcgcccgc cgcgggcgat
     3121 gacgaacgga tgcttcgtgt ggagggtgac gacttccgcc gacagggtca gagccatcgg
     3181 ttgcggtgca gccaccagag catcacggcg gaaatcgcgg ccatcgagcc gagcgccagg
     3241 tagaagccga gcgggtgatg ggtgaacggc atctggctga agttcatgcc gtagatgccc
     3301 cccaccacga tcagcggcag cgcgatggtc gagacgatgg acagctgctt catcacgcga
     3361 ttcatgcggt tggacgtctg ggagaggtag gtctcgagca cacccgccag cagatcgcgc
     3421 gtcgtatcga ccgactccat gatgcggatg gtatggtcgt acacgtcccg gtagtacagc
     3481 tgcgtctcgg agcggatgta gatcgacggc cggttggtga ggacgttcag cacctcgcgc
     3541 agcggcccga cgtgccgccg cagcccgaag gcggaacgct tcgccttgaa gatctcgtgg
     3601 atcagcttct cgtcgaacgc ctcgaacagc cgctcttcca gctggtccac ctgatcgttg
     3661 agtgattcca cgatcgggaa gtacgcgtcc accgcctggt cgatgatcga atgcatcgtc
     3721 atctcggcgc cgcgtcccag caggtccggg ctgcgcgtca ggcggtcgcg cgtgatgtcg
     3781 cagctcgggc tctccaccgc gtgcacggtg acgaggaaat tcttgcccag gaagaagtag
     3841 agcttgaagg tctcgagatc gaacggatcc ggcgtggcgc ggtcgaagcg gattcccgat
     3901 atcacgacga agatgtaacg gtcgtactcc tccagcttca cccgcgagcc cggcgacatg
     3961 gtgtcttcga tcgccagcgg gtggaacccg aagatcttct cgagcagcgc gtgctggtgc
     4021 acgtcggtgc tgtcgatgtc cacccacagc tccccttcgt cccgctcgag gatctcccgc
     4081 agccgcagcg gcggcagatc ctgctccagg gtcccgtcgg ggcggcgata cagactggcc
     4141 ggccagtgct tgggcgcggc ggcggcggga gcggccgggg agagggtcac gactcaagta
     4201 tcgggggcgg gccccgactc aggcaagcgc acgcaccagc gcggtgacac ccgcgaccag
     4261 cagatccagc cggtaccccc cttccagcac cgccgccacc ggccggccgt cgagccgggt
     4321 ccgcaatgcc gtcgtccagg tcgcgatgtc ctcgggggtc aaggtgaagc cgcccagcgg
     4381 atccccgccg agtgagtcga acccggccga gaggagcagc acgtcgggcg tccagcccac
     4441 cagggccgcg tccacggcct ccagcagatc gtggacgtac gtggcgcgcg gcagccccgg
     4501 cggacggggc acgttgaaca cgttccccac gccgcgctcg tcggccgcgc ccgtccccgg
     4561 gtaccaggga tactggtgca tcgagacgaa gcggatcgag gcgtcctgct ccaccagcgc
     4621 ctgcgtcccg ttgccgtggt gcacgtccca gtccacgatc agcacgcgtg ccgcgcccag
     4681 cgcctgggcg tgccgcgccg ccacgaccac gttgttgacg aagcagaacc ccatggcgcg
     4741 cgaggccagg gcgtggtggc cgggtgggcg ggtcgcggcg aaggccgtgc ctcccccgcg
     4801 cggccgtatc gcctcggtca cggcgcggag cgcgacgccg gcggaggccg tcacggcctc
     4861 ccagctgcgc ggccccatgt aggtgtcggg gtcgatcggg ccgccgcccg ccgccgccag
     4921 cgcctcgagg gcgttgaggt agcgccgcgg atgcaccagc tcgagcgcgg tgacgtcggc
     4981 ggacgtggct tcggcccagg agaccgccgg ggccagcgcc ggggcgcgca gcgcctccag
     5041 caccgcctcg atgcgctcgg gccgctcggg atgtccggct cgtcccggat gcagccgcgt
     5101 cgccgggtga taggtgacgg tggtgggcag aggctcagtt gtgaaggttt ttttcccgca
     5161 gcaggcgccg gatcgcttcc gcctcggcct tcatttccgc tcgcccggca tccaggtcca
     5221 ccagcgtgcg gcggtagcgc cgccagagga gcaccacggc cgcggccgcc agagcgcaag
     5281 cgatggcgcc ggccagcaga gaattgcgcc gctgctccag gccggcccac gccagcagca
     5341 cggcgtcgag cagcaggaac ccgacggcga acgtggccag gggccggccc cgcgtggtca
     5401 ccgcaccgcg cgccgccgcg ccgggagcac gatgaa
//
LOCUS       DBNGZW010000399         1200 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1537994, whole genome shotgun sequence.
ACCESSION   DBNGZW010000399 DBNGZW010000000
VERSION     DBNGZW010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1200)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1200)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1200
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1537994"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="AC16JC_09390"
     CDS             complement(<1..254)
                     /locus_tag="AC16JC_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164948.1"
                     /translation="MSATVPAGASGQLERPGFRRCPVLGRQIHLPAESLVKANAVVSI
                     VALLVGAIAAILLVLTRWQAVHLLPAEWFYRVLTAHGMSML"
     gene            complement(251..835)
                     /locus_tag="AC16JC_09395"
     CDS             complement(251..835)
                     /locus_tag="AC16JC_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008287723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HIR5164949.1"
                     /translation="MTPETHTGLDAPAGIWWKPAHRTEKLWVAIAFGWCMVLFAMMPL
                     WHLKGGQNPSGIRRRTTPAAYFQRTQEFIKQYKVGDDRGMPIVEPPAGSDVYLAGMTF
                     QWTPILRLRQGAEYTLHISSLDVNHGFSLFPVNVNFQVVPGYDYGLRVTPTEAGDYRI
                     ICNEFCGIGHHTMVGRVIVLDSAGREMARSQEAR"
     gene            complement(845..1018)
                     /locus_tag="AC16JC_09400"
     CDS             complement(845..1018)
                     /locus_tag="AC16JC_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164950.1"
                     /translation="MTQGSPAPKGDPPTIPLGQRLFDNVFLLLIAGIVVMAVLYTGWG
                     LWEVLTLPAAKLP"
BASE COUNT          197 a          391 c          418 g          194 t
ORIGIN      
        1 agcatgctca tcccgtgcgc ggtgagcacc cggtagaacc actcggcggg cagcaggtgc
       61 accgcctgcc agcgggtgag caccagcagg atcgccgcga tcgctccgac cagcagggcg
      121 acgatcgaga cgaccgcgtt ggccttcacc aggctttcgg ccgggagatg gatctgccgg
      181 cccaagacgg ggcaccggcg gaaaccgggg cgctcgagct ggccggacgc gccggccggt
      241 acggtggcgc tcatcgggcc tcctgggacc gggccatctc gcggcccgcg ctgtccagga
      301 cgatcacccg gcccaccatc gtgtggtggc cgatcccgca gaactcgttg cagatgatcc
      361 ggtaatcgcc ggcctcggtg ggcgtgaccc ggagcccgta gtcgtagccg gggacgacct
      421 ggaagttcac gttcaccgga aacagcgaga acccgtggtt cacgtccagc gacgagatgt
      481 ggagcgtgta ctcggccccc tgccgcagcc gcaggatcgg cgtccactga aacgtcattc
      541 ccgcgagata cacgtcgctg ccggccggcg gctccacgat cggcatgccg cggtcgtcgc
      601 cgaccttgta ctgcttgatg aactcctggg tgcgctggaa ataggcggcg ggggtggtgc
      661 ggcggcggat gcccgacggg ttctgcccgc ccttgaggtg ccagagcggc atcatggcga
      721 agagcaccat gcaccagccg aaagcgatgg cgacccagag cttctcggtg cgatgggccg
      781 gcttccacca gatgccggcg ggcgcgtcga gcccggtgtg cgtctcaggc gtcatggcgg
      841 ctcctcacgg cagcttcgcg gcgggaaggg tgaggacctc ccacagtccc cacccggtgt
      901 agagcaccgc catgacgacg atacctgcga tcaggagcag gaacacgttg tcgaacagcc
      961 gctggccgag cgggatcgtc ggcgggtcgc ccttgggtgc aggtgaaccc tgcgtcatga
     1021 cgcctccttg gagcggtcgg attgtcggtg ggattcacga caatgggtga cgaagcggtg
     1081 gcgcgcaagc ggggcggcgg cgtcttcata cgaaattcat tcgggcttca cactgacgcc
     1141 gtcggtgcac ctattattct gtccttcatg cctctcgccg atcatcctgg gcgtcggctc
//
LOCUS       DBNGZW010000400         7836 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_789774, whole genome shotgun sequence.
ACCESSION   DBNGZW010000400 DBNGZW010000000
VERSION     DBNGZW010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7836)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7836)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7836
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_789774"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..563)
                     /locus_tag="AC16JC_09405"
     CDS             complement(<1..563)
                     /locus_tag="AC16JC_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5164951.1"
                     /translation="MRLLIVEDDSELAGLLRDGFAKSQIEVTLAASFAEGSERARHGA
                     FDVLLLDVLLPGGSGFELCRRLRDEGVATPVLMLTAMDAVDDRVRGLEAGADDYLPKP
                     FAFPELLARVKALARRRPALTPARYRVADLEVDLAARTVRRGGRPLALTAKEFTLLEL
                     FVRHPGRVLDRATITAHVWDDNHDPFA"
     gene            complement(585..656)
                     /locus_tag="AC16JC_09410"
     tRNA            complement(585..656)
                     /locus_tag="AC16JC_09410"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(621..623),aa:Glu,seq:ttc)
     gene            complement(664..736)
                     /locus_tag="AC16JC_09415"
     tRNA            complement(664..736)
                     /locus_tag="AC16JC_09415"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(700..702),aa:Glu,seq:ctc)
     gene            complement(818..2473)
                     /locus_tag="AC16JC_09420"
     CDS             complement(818..2473)
                     /locus_tag="AC16JC_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164952.1"
                     /translation="MPALAVVVLLLAQSDSAALLSHARRAQADFERTRLFLLPPGFAH
                     SGERCDARIGRFCYWSDGADSHVPPEPAQIGVARRRLLEVLASTAQVLTGDAWVVGQR
                     VRYLLEAGRPLDAAIAAQNCRATAWWCAALEGLAWHGVGDTDAAERLFQAALDLMPED
                     ERCRWTDLSDLLEEPLRRRYRSLSCDERSGFDARVWWLAQPLWSLGGNDRRTEHYARL
                     TLAHLLEHARSPYGLWGDDQREMIVRYGWPLAWERDDGGGMHEPVAIGHEPEPSYHFV
                     PDDRTFDGRASGDDTGDLDWKAARERYAPAYAAAFSVLEPAFATFRRGESTLVVGTYD
                     VSRDTLYRRASREAALVLARDERSAPVIVRHPAAGPSGVLVAVAPWQPAMLSLELRAP
                     EGGAAARVRERPPALSPAAGAVTLSGILLFEPADSLPAGLAGALTSPHVGGITRGERI
                     GLYWEAYGLASGEDISTAVTVTPERTSWLRRAAATLRLAPHKSSVRVEWREGARLEQG
                     TAPRALVLDLAGLDAGRYRIEVAVSTAGRPAATTSRRIDVIEQ"
     gene            complement(2522..3238)
                     /locus_tag="AC16JC_09425"
     CDS             complement(2522..3238)
                     /locus_tag="AC16JC_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164953.1"
                     /translation="MRLSSVVACWCVVVLPPASRAQGVDTNTTAAVMWDMSQPLGDLH
                     RRMTPLRAPQPGDSARAAALLVNIRERVARYRDVRVADADGYRPFLRGVKQPVYHYTN
                     RRNALAERFRFDPAAPTSLLYRPDSTGGLVLIGVMYSEAPGATPEELDQRVPLSIARW
                     HQHVNLCLPPLGALARWRDSSSGRPVFGPRSPIATPDACAAAGGRFVPRLFGWMVHIN
                     AFVDDPAIVWGGHEMHEHPR"
     gene            complement(3345..3713)
                     /locus_tag="AC16JC_09430"
     CDS             complement(3345..3713)
                     /locus_tag="AC16JC_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0044183 - protein folding chaperone
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HIR5164954.1"
                     /translation="MRLRLAVLTSALVLLGSSVALAQGAPGPGGFAARRMQRLLQGIT
                     LTPEQQTKVDSITARYATQMPAFTPGAPPDSATRAKMRDLNQKQDGEVRALLTADQQK
                     VWDANVEAMRSRMQRPPGNR"
     gene            3905..5239
                     /locus_tag="AC16JC_09435"
     CDS             3905..5239
                     /locus_tag="AC16JC_09435"
                     /inference="COORDINATES: protein motif:HMM:NF018269.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoredictase family protein"
                     /protein_id="HIR5164955.1"
                     /translation="MRLTKWLAPLVLFCVYCNRPNPPPKPPPPPAGPDALTGFDNASN
                     GFVHDSIHEADRDAFDDVESVADGLGPLYNAQSCRECHQNPTSGGASQVSELRVGHLG
                     PDGRFRNPDLPIGRGTDTVPGRTLVNDRAICPSGAFPDSEIQQRVPDSETIRTFRASL
                     NVLGDGYVEAVADTTLFGIARQQRQQTNGKIRGQALLVPIVEAPGRTAVGRFGWKDQH
                     ASLLSFAGDAYLNEMGITTTLFPDEVVATCNTAAEPNDAPGPDGLSDVDRFARFMRAT
                     KAPARDSLLAATPAAAQGSQLFDSIGCVTCHVRTLVTAPEGTVINGGHDTLSAALGTK
                     AFHPFGDFLLHDVGTGDGIAIAVPEHYGRTFARFAWQAFSMDSLLGARNKVRTAPLWG
                     VRLRPRLMHDAASLTLRDAIVRHGGEASDVTSRFLRLSKSQQAAIVEFLKSL"
     gene            complement(5306..5644)
                     /locus_tag="AC16JC_09440"
     CDS             complement(5306..5644)
                     /locus_tag="AC16JC_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164956.1"
                     /translation="MARRRSRGPTSTASSTERHLELRYPFAGGLTPFAFAGGGAVTVD
                     QAHNNQVPTFTKPAGMLGAGIGYGIPRSNFEVFAEGKSLVYKWDRSGFDKTQWDVTYS
                     VGLAYRLTLH"
     gene            complement(5997..6965)
                     /locus_tag="AC16JC_09445"
     CDS             complement(5997..6965)
                     /locus_tag="AC16JC_09445"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5164957.1"
                     /translation="MGVSTHDIFGDAPGAQPATPGSYGEAPRGYRLPQGTRLGRVRLQ
                     IGDLTRSVAYYETTLGLRVLRRDDSRAQLGQQGADTPLVELRERAGARPVAHRSRIGL
                     FHFAILLPDRPALGRFVRHLGEIDERVGAGDHLVSESLYLQDPDGLGIEVYADRPRAT
                     WRRIGRELMMATDPLDVAGLVREAGVDPWTGMPDGTVMGHVHLHVGDLAAASAFYSEA
                     LGFDRMVWQYPGALFLAASGYHHHLGTNTWAGSGAKPPAAGEAQLLEWTIEVPDQASL
                     DAVSHSLVGAGYAAEWDCESGDARALVTRDHWGTPVRLQSATAPSA"
     gene            complement(6971..7127)
                     /locus_tag="AC16JC_09450"
                     /pseudo
     CDS             complement(6971..7127)
                     /locus_tag="AC16JC_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005820357.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
     gene            complement(7133..7675)
                     /locus_tag="AC16JC_09455"
     CDS             complement(7133..7675)
                     /locus_tag="AC16JC_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006604288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR5164958.1"
                     /translation="MTYRTVTAAAAAAVIVLIGVVVARPTGRVAAAPSQPADRCAERL
                     QTFDTLDFQVFRQQAWKRLSESHADNIVVTWPDGHETTGITRHIADLSAMFVYAPDIT
                     IAAHPIRICQGDYTAVTGVMTGTFSRPMPTANGGTIPPTGKSFKLTMATIGHWTGATM
                     DHEWLFWDNQEFMRQIGLGK"
BASE COUNT         1219 a         2751 c         2709 g         1157 t
ORIGIN      
        1 ttggcgaagg ggtcgtggtt gtcgtcccac acgtgcgccg tgatggtcgc acggtcgagc
       61 actcgccccg ggtggcgaac gaacagctcc agcagggtga attccttggc ggtgagcgcc
      121 agcggccgcc cgccgcggcg tacggttcgc gccgccaggt ccacctcgag gtcggccacc
      181 cggtagcggg ccggcgtgag cgccggtcgc cggcgcgcca gggccttgac ccgcgccagc
      241 agctcgggaa acgcgaacgg tttggggaga tagtcgtcgg caccggcttc gagcccgcga
      301 acgcgatcgt ccacggcgtc catggcggtg agcatcagta ccggagtggc gaccccttca
      361 tctcgcaggc gtcgacacag ctcgaaaccc gagccgccgg gcagcagcac gtcgagcagc
      421 aggacgtcga aggctccgtg gcgcgcccgc tccgatccct cggcgaagct ggccgcgagc
      481 gtcacctcga tctgcgactt ggcgaagccg tcccgcaaca gccccgcgag ctcggaatcg
      541 tcttcgacga tcagcagccg catgggcgaa gactagcgtg gccatgcccc caaggggaat
      601 cgaacccctg ttcccacctt gaaagagtgg tgtcctgacc gttagacgat gggggcggag
      661 gaatagcggt aacgggattc gaacccgtgt ttgagccttg agagggctcc gtcctagtcc
      721 cctagacgat accgccgcaa ccggcccaca catgtggaag gataccgggg ccgggcggag
      781 aatatcgcca gcccggcgcg gccttggcaa cccgcgctca ttgctcgatc acgtcgatac
      841 ggcgggaggt cgtggccgcc ggacggcccg cggtcgagac cgccacctcg atccggtacc
      901 ggcccgcgtc gagcccggcc aggtcgagca ccagggcccg cggcgccgtg ccctgctcca
      961 gccgggcacc ctcacgccat tccacccgga cgctgctctt atgcggggcg agccgcagcg
     1021 tggcggccgc gcgtcgcagc cagctggtcc gttcgggcgt caccgtcacc gccgtcgaga
     1081 tgtcctcgcc cgacgcaagc ccgtacgctt cccagtagag cccgatgcgc tctccgcggg
     1141 ttatcccgcc gacatgcggg ctggtcagcg cgcccgccag ccccgccggc agcgaatccg
     1201 ccggctcgaa cagcaggatg cccgacagcg tgacggcgcc ggcggccggc gagagcgccg
     1261 gaggacgctc ccgcacccgc gcggccgcac ccccttccgg cgcccgcagc tccagcgaca
     1321 gcatcgccgg ttgccacggt gccacggcca cgagcacgcc cgagggtccc gccgcgggat
     1381 ggcgcacgat cacaggtgcc gatcgctcgt cgcgcgccag cacgagcgcc gcctcgcgcg
     1441 acgcgcgccg gtatagcgtg tcgcgcgaga cgtcgtaggt ccccacgacc agggtggatt
     1501 ccccacgccg gaacgtcgcg aacgcgggct ccagtacgct gaaggcggcg gcgtacgccg
     1561 gggcatagcg ctcgcgcgcg gccttccagt ccaggtcccc ggtatcgtcg ccggatgctc
     1621 gcccgtcgaa cgtccggtcg tcgggcacga aatggtagct cggctccggc tcgtgaccga
     1681 tggcgaccgg ctcgtgcatg cccccgccgt cgtcccgctc ccaggccagc ggccatccgt
     1741 agcgcacgat catttcgcgc tgatcatcgc cccacaggcc gtagggcgag cgcgcgtgct
     1801 ccagcagatg cgcgagcgtg aggcgggcgt agtgctcggt gcggcgatcg ttcccgccca
     1861 gcgaccagag tggctgcgcg agccaccata cccgggcatc gaagccggat cgctcgtcac
     1921 acgagaggct gcgataacgg cgacgcagcg gctcctcgag cagatccgag agatcggtcc
     1981 agcggcagcg ctcgtcttcg ggcatgaggt ccaacgccgc ctggaacagg cgctcggcgg
     2041 cgtccgtgtc cccgaccccg tgccacgcca gcccctcgag cgccgcgcac caccacgcgg
     2101 tcgcgcggca attctgtgcg gcgatcgccg cgtcgagcgg ccggccggcc tcgagcaggt
     2161 accggactcg ctgccccacg acccacgcgt cgcccgtcag cacctgcgcc gtgctggcca
     2221 gcacctccag caaacgcctg cgtgcgaccc cgatctgcgc cggctccggg gggacgtggg
     2281 agtctgcgcc gtcgctccag tagcagaacc gtccgatgcg cgcgtcgcac cgctcacccg
     2341 agtgcgcaaa gcctggcggc aggagaaaca ggcgggtgcg ctcgaaatcc gcctgggcgc
     2401 gccgggcatg cgacaacagc gcggcggagt ccgattgggc gagtaacagc acgacaacgg
     2461 cgagtgcggg catgaacctg accccctcac cgtcaaccta ctccgggatt ggtgtgtggg
     2521 gctagcgggg gtgctcgtgc atctcgtggc cgccccagac tatggcggga tcgtcgacga
     2581 aggcgttgat gtgcaccatc cagccgaaca accggggcac gaagcggccc cccgcggcgg
     2641 cgcacgcgtc cggcgtagcg atggggctcc gcgggccgaa gacagggcgt ccgctcgacg
     2701 agtcgcgcca gcgcgcgagc gcgccgaggg gcgggaggca caggttcaca tgctggtgcc
     2761 accgggcgat cgaaagcggc acgcgctggt ccagctcctc gggcgtagcc cccggcgctt
     2821 cgctgtacat caccccgatc agcaccagcc cgccggtgga gtccggccgg tacaggagcg
     2881 acgtcggtgc ggcggggtcg aagcggaacc gctcggccag cgcgttgcga cggttggtgt
     2941 agtggtagac cggctgcttc accccgcgca ggaacggccg gtagccgtcg gcgtccgcca
     3001 cgcgcacgtc gcggtatcgg gccacgcgct cccggatgtt gacgagcaac gccgccgcgc
     3061 gggccgagtc gcccggttgc ggcgcgcgca gcggggtcat ccgccggtgg agatcgccga
     3121 gcggctggct catgtcccac atcactgcgg ccgtggtatt cgtgtccaca ccctgcgccc
     3181 ggctcgccgg gggcaggacc accacacacc agcaggcaac gacactcgac agtctcatga
     3241 ggcaggctcg ggttcgggtg tgggctatag acgcagaaac ccgcccctgt taacgcgacg
     3301 cagcgcgacc cttgcgagcc gcgctgcgcc ggattgaccg tgagctaccg gttgccgggg
     3361 ggccgctgca tccggctccg catcgcttcg acgttggcgt cccagacctt ctgctggtcc
     3421 gcggtcagca gggcgcggac ctcaccatcc tgcttctggt tcaggtcgcg catcttcgcc
     3481 cgcgtggcgg aatcgggcgg tgcgcccggc gtgaacgcgg gcatctgtgt cgcgtagcga
     3541 gcggtgatcg aatcgacctt cgtctgctgt tcgggggtca gggtgatgcc ctgcagcagc
     3601 cgctgcatgc gccgggcggc gaaccctccc ggcccggggg caccctgggc aagcgcgacc
     3661 gagctgccga gcagcacgag tgcgctggtg agaacggcga ggcgtaaacg catgaagtcc
     3721 ctccgacgga agtgttagtg tgttaccccc gttgtacgcg tccggggggc gtctcgttga
     3781 gcccctgcgc cctgcaagac cccatcaggg gtgactcggg tcacggactg gtgtaggatc
     3841 ctgggacaag gtgcatcgcg ccggctgccg cctccggacg tcccaccgtt ccccgaggtc
     3901 tctcatgcgc ctcacgaaat ggctcgcccc gctggtgctg ttctgtgtgt actgcaaccg
     3961 acccaatcca ccacccaagc ctcccccgcc gcctgcggga ccggatgccc tcaccggatt
     4021 cgacaacgcc agcaacggct tcgtccacga ctcgattcac gaagcggacc gtgacgcctt
     4081 cgacgacgtg gagtcggtcg ccgacggcct cgggccgctc tacaacgcgc aatcgtgccg
     4141 cgagtgtcac cagaacccga cctcgggcgg cgccagccag gtgagcgagc tgcgcgtcgg
     4201 ccacctcggg cccgacgggc ggttccgcaa ccccgatctc ccgatcggcc gcggcaccga
     4261 caccgtcccg ggccgcaccc tggtcaacga ccgggcgatc tgccccagcg gagcattccc
     4321 cgacagcgag atccagcagc gggtccccga ctcggagacc atccgcacgt tccgggcgtc
     4381 cctcaatgtc ctgggtgacg gctacgtcga agcggtggcc gacacgacgc tgttcggcat
     4441 cgcgcgccag cagcggcaac agaccaacgg caagatccgc ggccaggcgc tgctggtgcc
     4501 catcgtcgag gcgccgggca ggacggcggt gggacggttc ggctggaagg accagcatgc
     4561 gagcctcctg tcgttcgccg gagatgcgta cctgaacgag atgggaatca ccacgacgct
     4621 gttccccgac gaggtggtcg ccacctgcaa cacggccgcc gagccgaacg acgccccggg
     4681 cccggacgga ctgtcggacg tggaccggtt cgcgcgcttc atgcgggcga ccaaggcccc
     4741 cgcccgcgac tcgttgctgg ctgcgacccc ggccgcggcg caagggtcgc agctgtttga
     4801 cagtatcggg tgcgtgacct gccacgtgcg gaccctggtc acggcacccg agggcaccgt
     4861 catcaacgga gggcacgaca ccctctcggc ggcgctcggc accaaggcgt ttcatccgtt
     4921 cggggacttc ctgctgcacg acgtgggaac cggggacggg atcgccatcg cggtgccgga
     4981 gcattacggc cggacgttcg cccgattcgc ctggcaggcg ttctcgatgg acagcctgct
     5041 cggggcgcgc aacaaggtgc gcaccgcgcc gctgtggggg gtgcgtctgc gcccgcgttt
     5101 gatgcacgac gccgcctcgt tgacgctccg ggacgcgatc gtgcgccacg gcggcgaagc
     5161 gagcgacgtg acgagccggt tcctgcgcct cagcaagagc cagcaggcgg caatcgtcga
     5221 gttcctgaag tcgctgtgag atgacgagaa acggggtggc ggagcaatcc gccaccccgt
     5281 cttcgccaag ctgtgctccc gcgcactagt gcagcgtgag ccggtaggcc aaccccactg
     5341 aatacgtcac gtcccactga gtcttgtcga agccggaacg atcccacttg taaaccaggc
     5401 tcttcccctc cgcgaagacc tcgaagttgg accgtgggat cccgtagccg atgcccgcgc
     5461 cgagcatccc ggccggcttg gtgaacgtcg gcacctggtt gttgtgggcc tggtcgacgg
     5521 tgacggcgcc accgcccgcg aaggcgaacg gcgtgagtcc acccgcaaac ggatagcgca
     5581 gttcgaggtg ccgctccgta gaagaggcgg tggatgtcgg cccccgcgaa cgacgacgtg
     5641 ccacgcgcct gcgcgtgcgc gaacgtgaag tcggcgtgca gggcgaagta cttggtgaac
     5701 tcccgcccga cactcagccc gaagttgtaa cccggcttga agtccgcggt cggcgtgccg
     5761 ctggtgttca ggttggcgag gtggttgtac ccgccgccgt tgatgttgaa gacgatcgcc
     5821 ccttgaccgg cgagcggcgc cgcgccgagc agcgcgacga ccgcgatggg tgtgccacga
     5881 tggaacgaca taagtgcctc cttctgatga gctgcacccg gcccagcgcc ggggcgagag
     5941 gagggtacgg gaggagcgat gcaaccgcgg tgagttcagt tcgaaagact cggcgatcag
     6001 gcgctgggag ccgttgcgga ttgcagcctg acgggagtcc cccagtggtc gcgcgtcacg
     6061 agcgcgcggg catctcccga ttcgcagtcc cactccgccg cgtatcccgc acccacgaga
     6121 ctgtgcgaca cggcgtcgag gctcgcctgg tcgggcactt cgatggtcca ctcgagcagc
     6181 tgcgcttcgc ccgccgccgg cggcttggcg ccactgcccg cccaggtgtt ggtccccaga
     6241 tggtggtggt agcccgaggc cgcgaggaac agcgccccgg ggtactgcca gaccatgcgg
     6301 tcgaagccga gtgcctcaga gtagaaggcc gaggcggcag cgaggtcccc cacgtgcagg
     6361 tgcacgtggc ccatcaccgt gccgtccggc attccggtcc aggggtcgac gcccgcctcg
     6421 cggacgagtc ccgccacatc cagcggatcg gtcgccatca tcagctcgcg gccgatgcgc
     6481 cgccaggtgg cgcgcgggcg gtcggcgtag acctcgatgc cgaggccgtc gggatcctgc
     6541 aggtacagcg actcgctgac cagatgatcc cccgcgccca cccgctcgtc gatctcgccg
     6601 agatggcgga cgaaccgccc cagcgccggc cgatccggca acaggatggc gaagtggaac
     6661 agcccgatac gactccgatg cgctaccggc cgcgcgccgg cacgctcccg cagctcgacg
     6721 agcggcgtgt cggcaccctg ctgccccagc tgcgcgcgcg agtcgtcgcg gcgcagcacg
     6781 cgcaggccca gcgtcgtctc gtagtacgcg accgagcggg taaggtcgcc gatctggaga
     6841 cgcacccgtc cgagtcgcgt cccctggggg aggcggtagc cgcgcggcgc ttcgccgtag
     6901 ctgcccggtg tcgccggctg tgcgcccggc gcgtcaccga agatgtcgtg ggtcgagacg
     6961 cccatggaga ttactccgcc aatccgtcgt tgaacttgat gctggcatga ctgccgttgc
     7021 agaacggctt gttggcgtag gcgccgcagc ggcacagcgt cacccggtta tcgggtttcg
     7081 tactgtttgt catccgccga ttcgatggtg atgccgccgc gcacccaccg gattacttgc
     7141 ccaggccgat ctgccgcatg aactcctggt tgtcccagaa cagccactcg tgatccatgg
     7201 tcgcaccggt ccagtggccg atggtggcca tggtgagctt gaacgacttt ccggtcggcg
     7261 ggatcgtccc gccgttcgcg gtgggcatcg gacgggagaa cgtcccggtc atgaccccgg
     7321 tgaccgccgt gtagtcaccc tggcagatgc ggatggggtg ggccgcgatg gtgatgtcgg
     7381 gggcgtagac gaacatcgcg ctcaggtccg cgatgtgccg cgtgatgccg gtcgtctcgt
     7441 ggccatcggg ccaggtcacg acgatgttgt cggcgtggct ctccgacagt cgtttccacg
     7501 cctgctgcct gaagacctgg aagtccagcg tgtcgaaggt ctgcagccgc tcggcgcagc
     7561 ggtcggccgg ttgggacggg gcggccgcga cccgtccggt cgggcgggcc accacgaccc
     7621 cgatgagcac gattacggct gccgccgcgg cagcagtcac ggtccggtag gtcatacctt
     7681 cctcctgtaa tgcgggtgcc gcgacggcgc ggcgttcgtt cgctcggaaa tagacgaacg
     7741 gtatccaacg gcaagtaggt acccataggt aaccatttcg ccggcggcgc gcgcggcgta
     7801 cgtttggcga catgacgctc gggcgatcgt tgcatc
//
LOCUS       DBNGZW010000401         6328 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_96231, whole genome shotgun sequence.
ACCESSION   DBNGZW010000401 DBNGZW010000000
VERSION     DBNGZW010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_96231"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1071
                     /locus_tag="AC16JC_09460"
     CDS             <1..1071
                     /locus_tag="AC16JC_09460"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR5164959.1"
                     /translation="PGDRSFPGRVSPIMISQSPSFVLTAGELPGTPSSNAEVPEDGEL
                     FDAYSRAVVHATERVAPAVVSVESHARRGRSGRQRDGGGSGFVFTPDGFVLTNSHVVQ
                     GASKLSVVLHDGRRHDADLVGDDPDTDIAVLRVSAGELPVAPLGDSHRLRVGQLVIAI
                     GNPYGFQSSVTAGVVSALGRTWRSVTGRRIEDVIQTDAALNPGNSGGPLVNSRGEVIG
                     VNTAVILPAQGICFAVGVNTAKVVAAALLKDGRIRRAWIGVAGQNVTLGKPSVRRHAL
                     AGAGGVLVQSVEPGSAAARAGVAPGDIIVAMGEEAVGGVDDLHRMLLADRIGVTGTVT
                     VLRRGERRDLTIVPEERPAPGR"
     gene            1068..2489
                     /locus_tag="AC16JC_09465"
     CDS             1068..2489
                     /locus_tag="AC16JC_09465"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR5164960.1"
                     /translation="MTPFDVTLALVGVVIVVAALLSGVVDRFRVPQVALFLALGAALG
                     PFGMGLLDFGLTSPVLEGVATIGLVLVLFTDAIALDLRTVRGQARLAFLLLGPGTLLT
                     AGFIAFAAWALLGLTPALAIILGAALASTDPVMIRSLLRGETAPAAARQALQLESGLN
                     DLFLLPIVLLAMTLGGGSAATTPVARQTVGLLLLGPGAGVLVGFLGVAVLEGVRKRAG
                     VRRDYESIYALGLAFAAFAAAEAVHGSGFLAAFAAGLTVAGFDAELCDCFVDYGQATA
                     EMFLLFTFVAFGASLVWTGLEVIGWRTLVFTLVALFSRSLVLGVALRRQPLDARSRRL
                     IVWYGPRGLSSLLLVLLPVFAGVPGATTLFPVAALVVLASVVIHGGAMMIGDGGRGTR
                     DELGEGGRGKGEGGDKITLDEVKRLQSQGERVLLLDVRKEGAWRESEVKAQGAIRVPP
                     DNARARAAELALPRHDWLVAYCA"
     gene            complement(2724..4601)
                     /locus_tag="AC16JC_09470"
     CDS             complement(2724..4601)
                     /locus_tag="AC16JC_09470"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR5164961.1"
                     /translation="MLFLITAASLGLQQSVSDTSPFRALPLPAATRVRSSSGAPGPDY
                     WQQEASYTIRATLDTGPSLLTGTETIHYVNHSPDALAFVWIQIEQNIFAKNSVTYQLN
                     QPPLHFAGGAVFDFTGKGFIGGITIDRFTAAGKELERTEYGTMMRVELPVPLVPGAAI
                     DFDVAWHFPVPPYGGGRMGRIGNRLYEIGQWYPRLVVYDDVNGWNPLPYIGAGEFYLE
                     YGDYDVTVTLPAGYLVAATGTVQNPTEVWSPAIRQRLAQAKTAAERVQVVTKAEATTN
                     GARQTAGTKAWHFTASNVRDFAWAASPDFRWDASAWNGIQINTFYRPAAAPWEEANRM
                     ARFTIQHFSETWGGYPWPHATTVEGLIEGMEYPMLTFVPSIEKREDQFWVLMHEFGHE
                     WFPMTVGSDERRYPWMDEGFNTFIDYGGAEGYFKGTAYGDTVRRELLTALKVSDVPGQ
                     EQPLITKPVEQRDLAWGAYQKPALMLTVLRDQVLGRETFERAMREYVRRWSFKHPQPA
                     DFFRTIANVSGRDLDWFWREWIYTTARLDQAVDSVRAVGRDSIYVFLSNRAQMVLPVT
                     LQLRYADGTTDTKVLPVEMWYLGDRFTARFATPKAVTGVVLDPTASLPDMNRENNRWT
                     R"
     gene            complement(4739..>6328)
                     /locus_tag="AC16JC_09475"
     CDS             complement(4739..>6328)
                     /locus_tag="AC16JC_09475"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5164962.1"
                     /translation="SWARLGYPGPLGPPPPTPKRIHPTDVTGDTTLDCDVVVVGSGAG
                     GGVMAGELAARGKDVIVLEKGGYYNEADFTQYEGEMLSRLYDAGGLLTSDDLGFVILQ
                     GSCLGGGTVINYTTAFHTPAAVCEQWDRERGLHHFSSHEFSASLDAVAQRLSVGTEEN
                     RPSARDAALIRGLEALGWHHGLLPRNVRGCSQEDDCGYCGYGCRRSAKQSTLVTYLQD
                     AFDRGTRILVGADARRVQFERGRATGVVATVRHPQAARSYTVTVRARAVVVAAGSIHS
                     PALLLRSGVELPALGRFLALHPGTAVFGTMPRETRPWAGTLQAHYSDQFVDLDAGYGF
                     KLETVPMHPATFALAVPWESAAQHADAMAALSRTALVAILLRDREGGRVRVDGDGTPV
                     VSYRLSAYDAAHLRRGLAEGARLLEAAGAQEIWAPHARWIAYRPDGAGSARERWLERM
                     DRAGYAPNQIQLATFHQMASCRMGTSPRDSVVSAEHEVWSSPGLYVADASTFPSASGV
                     NPALTIMGIAHRAAGIIAAKI"
BASE COUNT          908 a         2091 c         2315 g         1014 t
ORIGIN      
        1 cccggggacc gttcgttccc gggacgtgtt tcaccgatca tgatctccca atctccatca
       61 ttcgtgttga cggcaggcga actgcccggc acgccgtcct cgaacgctga ggtccccgaa
      121 gacggcgagc tgttcgacgc gtattcccgc gcggtggtgc acgccacgga gcgcgttgcg
      181 ccggccgtcg tgagcgtcga gtcgcacgcg cggcggggac gttccggccg ccagcgcgac
      241 gggggcggct cgggattcgt gttcaccccg gacggcttcg tgctgaccaa cagccacgtc
      301 gtacagggcg cgtcgaagtt gagcgtggtg ctccacgatg ggcgccggca cgacgccgac
      361 ctggtcggcg acgatccgga caccgacatc gccgtgctgc gcgtgagcgc gggcgagctg
      421 ccggtcgccc cgctcggcga ttcgcaccgc ttgcgcgtcg gtcagctcgt catcgccatc
      481 ggaaatccct acggcttcca gagctccgtg acagccgggg tggtcagcgc cctgggacgc
      541 acctggcgct cggtgaccgg acggcgtatc gaggatgtga tccagacgga cgccgccctg
      601 aaccccggca actcgggggg accgctcgtg aactcccgcg gtgaggtgat cggcgtgaac
      661 acggcggtga tcctgcccgc gcaggggatc tgtttcgcgg tcggggtgaa cacggcgaag
      721 gtcgtggccg cagcgttgct caaggacggc cgtatccgcc gcgcctggat cggcgtcgcc
      781 gggcagaacg tgacactcgg caagccgtcg gtgcggcgcc atgcgctcgc gggcgccggc
      841 ggcgtgctgg tgcagtccgt ggaaccgggt tccgccgcgg cccgcgccgg ggtggctcca
      901 ggggacatca tcgtggccat gggggaagag gccgtgggag gcgtggacga tctgcaccgg
      961 atgctgctcg ccgatcgcat cggcgtgacg ggcacggtca cggtgctgcg gcgcggcgaa
     1021 cgccgcgacc tgacgatcgt gccggaggag cggccggcac cggggcgatg acgcccttcg
     1081 acgtcaccct ggcgctggtc ggcgtggtca tcgtcgtggc cgcgctgctg tccggagtgg
     1141 tggaccgctt ccgcgtccca caggtggcgt tgttcctcgc cctgggcgct gccctcgggc
     1201 cgttcggcat gggcctgctc gatttcgggc tgacctcgcc agtgctcgag ggggtggcca
     1261 cgatcggtct cgttctcgtg ctgttcaccg acgccatcgc cctggatctg cgcaccgtcc
     1321 gcggccaagc ccggctggcg tttctgctgc tcggccccgg gacactcctg acggcgggct
     1381 tcatcgcctt cgcggcgtgg gcgctgctcg gcctgacgcc ggcgctggcg atcatcctcg
     1441 gcgccgcgct ggcgtcgacc gatccggtga tgatccgcag cctgctgcgg ggcgagaccg
     1501 ccccggccgc ggcgcggcag gcgctgcagc tcgagagcgg gctcaacgat ctcttcctgc
     1561 tgcccatcgt gctgctcgcc atgacgctcg gcggtgggag cgccgctacc acgccggtcg
     1621 cacggcagac ggtgggactg ctgctgctgg gaccgggggc gggcgtgctc gtcggcttcc
     1681 tgggggtggc ggtgctcgag ggtgtccgca agcgggccgg cgtccgccgg gactacgaat
     1741 cgatctacgc cctgggactg gccttcgcgg cgttcgccgc cgccgaggcg gtgcacggca
     1801 gcggcttcct ggccgcgttc gccgccgggc tcacggtcgc gggattcgac gccgagctgt
     1861 gcgactgctt cgtggactac gggcaggcga ccgcggagat gttcctgctg ttcactttcg
     1921 tggcgttcgg cgcctcattg gtctggacgg gactcgaggt gatcgggtgg cgcacgctgg
     1981 tcttcaccct ggtggcgctg ttcagccgct cgctggtgct cggcgtggcg ctgcggcgcc
     2041 aaccgctgga cgcccgcagc cggcggctga tcgtgtggta cggcccgcgc gggctctctt
     2101 cgctgctgct cgtgctgctg ccggtgttcg caggcgtgcc gggcgcgacg acgctctttc
     2161 ctgtcgcggc gctggtcgtc ctcgcgtcgg tcgtcatcca cgggggggcg atgatgatcg
     2221 gggacggggg acgcgggacg cgggacgagt tgggggaagg gggaagaggg aagggggaag
     2281 ggggagacaa gattacgctc gacgaagtga agcggctgca gtcgcaggga gagcgggtgc
     2341 tgctgctcga cgtccggaaa gagggcgcgt ggcgcgagag cgaagtgaag gcccagggcg
     2401 cgatccgggt gccgcccgac aacgcgcgcg cccgtgccgc cgagctggcg ctgccacgcc
     2461 acgactggct cgtcgcctac tgcgcctgaa acaacgaagc gacaagcgcc cgggtggcgc
     2521 gagagctgcg gctggcgggg tacgaaaaag cccgggcgct ggtgggcggg tgggcgctct
     2581 gggaagcggc ggggatgccg acagaaaaga agacgtcgag ctgactacgt cgagcaagca
     2641 cgtcgagctg ggacgtcaga cacgtctctg cttgatgcct ctgctcgacg tccttgctcg
     2701 acgtccttgc tcgacgtcct tgcctatcta gtccaccgat tgttctcccg attcatgtca
     2761 ggcagactcg ccgtcggatc cagaaccaca cccgtcaccg ccttcggcgt cgcgaaccgt
     2821 gccgtgaacc ggtctcccag gtaccacatc tcgaccggca ggaccttggt gtcggtggtc
     2881 ccgtcggcgt agcgcagttg cagggtgacg gggagcacca tctgggcccg gttcgacagg
     2941 aagacgtaga tcgagtcgcg acccacggcc cgcacggagt ccacggcctg gtcgagccgc
     3001 gcggtggtgt agatccactc gcgccagaac cagtcgaggt cgcggcccga gacgttggcg
     3061 atggtgcgga agaagtccgc gggctgcggg tgtttgaagg accaacgccg cacgtactcg
     3121 cgcatggcgc gctcgaaggt ctcgcggcca agcacctggt cgcgcagcac ggtgagcatc
     3181 agcgccggct tctggtaggc gccccaggcc aggtcgcgct gctcgaccgg cttggtgatg
     3241 agcggctgct cctggccggg aacgtccgag accttgagcg cggtgagcaa ttcgcggcgc
     3301 accgtgtcgc cgtaggcggt ccccttgaag tacccttccg cgccgccgta gtcgatgaag
     3361 gtgttgaacc cctcgtccat ccagggataa cgccgctcgt cggagccgac ggtcatcggg
     3421 aaccactcgt ggccgaactc gtgcatcagc acccagaact ggtcctcgcg cttctcgatc
     3481 gacggcacga aggtgagcat cgggtactcc atgccctcga tcaggccctc gaccgtcgtc
     3541 gcgtgaggcc agggatagcc gccccaggtc tcgctgaaat gctggatagt gaagcgcgcc
     3601 atgcgattcg cttcttccca cggcgcggcg gccgggcggt agaacgtgtt gatctggatg
     3661 ccgttccagg cactcgcatc ccaacggaag tcgggcgagg cggcccaggc gaagtcgcgc
     3721 acgttgctgg ccgtgaaatg ccaggccttg gtgccggccg tctgccgtgc gccgttcgtg
     3781 gtcgcttcgg ccttcgtgac gacctgcacc cgctcggccg ccgtcttcgc ctgcgcgagc
     3841 cgctgccgga tcgcgggtga ccagacctcg gtggggttct gcacggtgcc ggtcgcggcc
     3901 acgaggtagc ccgccggcaa ggtcaccgtc acatcgtaat cgccgtactc caggtagaac
     3961 tcgccggcac cgatgtaggg cagcgggttc cagccgttga cgtcgtcgta cacgacgagc
     4021 cgtgggtacc actggccgat ctcgtagagc cggttgccga tgcggcccat gcggccgccg
     4081 ccgtacggcg gcaccgggaa atgccaggcg acgtcgaagt cgatcgccgc accgggcacg
     4141 agcggcaccg gcagttcgac gcgcatcatg gtgccgtact cggtccgctc gagctccttg
     4201 ccggccgcgg tgaagcggtc gatagtgatc ccgccgatga accccttgcc cgtgaagtcg
     4261 aacaccgcgc cgcccgcgaa gtgcagcggc ggctgattca gctggtaggt cacgctgttc
     4321 ttcgcgaaga tgttctgctc gatctggatc cacacgaacg ccagcgcgtc gggcgagtgg
     4381 ttgacgtagt ggatcgtctc ggtcccggtg agcaggctcg gcccggtgtc gagggtggcg
     4441 cggatcgtgt agctcgcctc ctgctgccag tagtcgggcc cgggggcgcc gctgctcgag
     4501 cgcacccggg tggccgcggg aagcgggagc gcgcggaagg gggaggtgtc ggagacggac
     4561 tgctggagcc ccagggaagc ggcggtgatg agaaaaagca ttcggatctc ccgaaaagag
     4621 aattgcggat tgcggattgt ggattgcgga ttggtcttga cctcggtctg ggttccgaac
     4681 tccctcccat ccaatccgca atccgcaatc cacaatccac aatgtcgatg caccgaactc
     4741 agatcttagc cgcaatgatc cccgccgccc gatgcgcgat ccccatgatg gtcagcgccg
     4801 gattgacgcc ggaggcggat gggaacgtgc tcgcgtcggc gacatagaga cccgggctgc
     4861 tccacacctc gtgctcggcg ctcacgacgg aatccctggg gctggtcccc atgcggcacg
     4921 acgccatctg gtggaaggtg gccagctgga tctggttggg ggcgtacccg gcgcgatcca
     4981 tccgctcgag ccagcgctcc cgcgccgagc cggcgccgtc gggccgatag gcgatccagc
     5041 gcgcgtgagg cgcccagatc tcctgcgccc cggcggcttc gagcaggcgg gcgccttcgg
     5101 cgagcccgcg gcgcaggtgc gcggcatcgt acgcggacaa gcggtaggac acgacgggcg
     5161 ttccgtcgcc gtcgacgcgc acccggccgc cctcgcggtc gcgcagcagg atcgccacga
     5221 gggccgtccg cgacagcgcc gccatcgcgt cggcgtgttg cgcggcggac tcccagggca
     5281 cggccagggc gaacgtggcc ggatgcatcg gcacggtctc gagcttgaac ccgtaccctg
     5341 catcgaggtc gacgaactgg tccgagtagt gggcctgcag cgtgccggcc caagggcgcg
     5401 tctcccgggg catcgtgccg aacaccgcgg tcccgggatg gagcgcaagg aaccgtccga
     5461 gggcggggag ctccacaccg gatcggagca gcaaggcggg ggagtggatc gagcctgccg
     5521 ccacgaccac ggcccgtgcg cgcaccgtca cggtgtagga gcgtgcggcc tgggggtggc
     5581 gcacggtcgc gacgacgccg gtggcgcggc cgcgttcgaa ttgcacccgg cgcgcgtcgg
     5641 cgcccaccag gatgcgggtg ccacggtcga acgcgtcctg caggtaggtc acgagggtgg
     5701 actgcttcgc gctgcggcgg cagccgtagc cgcaatagcc gcagtcgtct tcctgggaac
     5761 acccgcgcac gttgcgcggc agcaggccgt gatgccagcc cagcgcctcg aggccacgga
     5821 tcagggcggc atcccgcgcg ctgggacggt tctcctcggt cccgacgctg aggcgttgcg
     5881 cgacggcgtc gagcgaggca ctgaactcgt gactcgagaa atggtgcagc ccgcgctcgc
     5941 ggtcccattg ctcgcacacg gcggccgggg tgtggaacgc ggtcgtgtag ttgatgacgg
     6001 tgccgccgcc gagacagctc ccctgcagga tgacgaagcc gagatcgtcc gaggtgagca
     6061 ggccgccggc gtcgtacagg cgcgacagca tctcgccttc gtattgggtg aagtcggcct
     6121 cgttgtagta gccgcccttc tcgaggacga tcacgtcctt gccgcgcgcg gccagctccc
     6181 cggccatcac gccgccgccg gcgcccgagc cgaccaccac gacatcgcaa tcgagcgtcg
     6241 tgtcgcccgt gacatcggtg ggatggatgc gtttgggggt gggcggtggg ggcccgaggg
     6301 gtccgggata tcccagccgg gcccagga
//
LOCUS       DBNGZW010000402         5419 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_96265, whole genome shotgun sequence.
ACCESSION   DBNGZW010000402 DBNGZW010000000
VERSION     DBNGZW010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5419)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5419)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5419
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_96265"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="AC16JC_09480"
     CDS             <1..472
                     /locus_tag="AC16JC_09480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164963.1"
                     /translation="GLRRTAVQAALAGAAVRPVDDSPRRILLRNSAYALRVAAWLRAH
                     STMEDRILVEFPAAVYLYTGRLSIPSAPAELRFGVSAFAVPGGYLAGHILADSVTIVV
                     VAAPPLPLARDVAAVRAACPRVLQWAGDGPPDTFPALYRVDSDRTCLTAHWNRL"
     gene            647..2272
                     /locus_tag="AC16JC_09485"
     CDS             647..2272
                     /locus_tag="AC16JC_09485"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF051540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR5164964.1"
                     /translation="MPGPRALLRFMYLWRFSVAIAMYLSAALRVRVVAPLDLLVTSIL
                     LFATLGFTSASYWYSHVRNRPPGRTFLYLQALYDVALVTTVVHVTGGPDSDFQSLYVP
                     VIAVTAVLMPPASTALVTALAALAYLADLLWGHHAAVNGTLWIQLGLFGLVAAVPAYF
                     SSRIKVMSAEHEALAVELRQARLEASDVLHNLASGVLTVDADGKLLFANPAAEQILGF
                     RAKDWLDRAFLPELARTSPEFWAAVAATARRGIRLMRVEATVRRGERSFPVGLTTTTI
                     AGNAGLPPRVTAIFTDIPDSKRLEELHLRAERLEAVAELSAGLAHEIKNPLASIRSSV
                     EQLSRSTRTNPDERFLASLIVRESDRLSRLLSEFLDFSRVRVTEYRAVDLRDVAGAAV
                     RLVRQHPDCPADVQIEFVGGPTPMEGDEDLLHRVVSNLVLNAVQASGPGVHVRVFTGR
                     PKPEEVPAGFTMENPVALRVSDNGPGIPEAVRERLFEPFVTGRQGGTGLGLAIVQRAV
                     AAHRGMVLVDTTPGHGTTFTVFFPAARRKEEAA"
     gene            2269..3657
                     /locus_tag="AC16JC_09490"
     CDS             2269..3657
                     /locus_tag="AC16JC_09490"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5164965.1"
                     /translation="MTGSPAAPTLLIVDDESAILDALRILLKNEGFDVLTAQGGKAGL
                     EQLRNAAPDIVLTDVRMPLVTGLDILTAVRQQDPETPVILMTAQASLQTAIQAVNEGA
                     FYYIQKPFSNDDLVAICRRAAEHRRLRAENKQLKQEIRRRERSRFAKHLGRSRLFADV
                     LRLAEQVAPTESTVLIQGESGTGKEVIARYIHDLSSRNEGPFLSLNCGALPESLLESE
                     LFGHVKGSFTGAVRDKQGLFAAARGGTFFLDEIGEMSPATQVKLLRVLQEREAIPVGG
                     TEAIPVDVRVVAATNRDLEDEIRQGRFRTDLYYRLNVIAIHLPPLRERRDDIEIFVTA
                     FLERIAKEKSTPVKHLMPEAAEAVLSYDWPGNVRELENALERAVVLTKGEAIPLSAMP
                     AKVTERRVEPLVADRVHHNPTLDTIERAYIAWVLQAEGGNKSRAAEVLGIDPSTLYRK
                     LSRYEGGSDGPI"
     gene            3644..4366
                     /locus_tag="AC16JC_09495"
     CDS             3644..4366
                     /locus_tag="AC16JC_09495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5164966.1"
                     /translation="MARSEAADPVGIETLERLAAAAPNYNRWLFERVGRWVGRRVLEI
                     GAGVGTMSAFLMNGARERVVLSDTDAHYLELLRERYARHPYVGVARLRLPTYEEALAR
                     ESFDTIICLNVLEHVRDDLLSLGTMFRLLQPGGRLVLLVPALPAIYGELDRALGHFRR
                     YRPGDLRRRYAAAGFVMRHLEYFNLAGIPGWWLTGRVLKRGLIPTASLRWYDALVPLF
                     RLERLLPWRIGQSLIAVGERPS"
     gene            4363..5124
                     /locus_tag="AC16JC_09500"
     CDS             4363..5124
                     /locus_tag="AC16JC_09500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009107521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5164967.1"
                     /translation="MTGPAAEGAPVLSVVVPAFNEATTVETVVRRVRDVPLRLEVIAV
                     DDASADGTGDVLDRLHREGLLDRVIHHSVNRGKGAALRSGFAVVTGDVVVVQDADLEY
                     DPSDLPALLEPIRRGRADAVYGSRFQGGPHRVLFFWHAAGNLILTLLSNMLTNLNLSD
                     METCYKVVRRDLLQRLPLTSDRFGFEVELTARLAQAGARIWELPISYSGRTYAEGKKI
                     TWRDGFAALLHILRFNLFPPGGDWREPRRADGAPR"
     gene            5108..>5419
                     /locus_tag="AC16JC_09505"
     CDS             5108..>5419
                     /locus_tag="AC16JC_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164968.1"
                     /translation="MALLAERLSGRRLYLVVGAVALVVYLGALGNRFAMDDLPLVVRN
                     PLVQSVSGMWQAFAAPFWPPDFGGHMYRPLAVATWALDQRVDGARWFHLVNLLWHAAA
                     CV"
BASE COUNT          753 a         1891 c         1870 g          905 t
ORIGIN      
        1 cggcctgcgg cgcacggcgg ttcaggccgc gctggccggc gccgccgtgc ggcccgtcga
       61 cgactcaccg agacgcattc tcctccgcaa cagcgcgtac gcattgcggg tcgctgcctg
      121 gctcagagcc cacagcacga tggaggaccg tatcctcgtc gagttcccgg ccgccgtgta
      181 tctctacacc ggccgcctct cgatcccgtc ggcacccgcc gagctccggt tcggcgtcag
      241 cgcgttcgcc gtcccgggcg gctacctcgc tgggcacatc ctggcagaca gcgtgacgat
      301 tgtcgtggtg gcggctcctc cgctgccgtt ggcgcgggat gtcgcggcgg tgcgggcggc
      361 gtgtccccgc gtgctgcagt gggccggcga tggcccgccc gacaccttcc ccgcgctgta
      421 ccgagtcgat tcggaccgga cctgcctgac cgcgcactgg aatcggctct agccctcgtt
      481 gtcctcggct tgccgcagcg cagggctttg ccggttcttg cattctgcca cagccggtcc
      541 aatacttacg ggcccgggcc gcccctcggc gacgacgtgc catctaattc attgtcggaa
      601 ttgaggttgg atgcgttggt tcggtaggtc ctccaaagcg tcggttatgc cgggcccgcg
      661 ggccctgctc cgcttcatgt acctgtggcg attctcggta gcgatcgcga tgtacctcag
      721 tgccgccctg cgcgttcggg tggtggcgcc gctcgatctg ctggtcactt ccatcctgct
      781 gttcgcgacg ctcggcttca ccagtgcctc ctactggtac agccacgtcc gcaaccgccc
      841 gcccgggcgc acgttcctct acctccaggc gctgtacgac gtggcgctcg tcacgaccgt
      901 cgtgcacgtc accggcggtc ccgacagcga tttccagagt ctgtacgtgc ccgtcatcgc
      961 cgtgactgcc gtgctgatgc ctcccgccag caccgccctc gtgaccgcgc tcgcggcgct
     1021 cgcctacctc gcggatctgc tgtggggaca tcacgcggcc gtgaacggca ccctgtggat
     1081 ccagctgggc ctgttcgggc tggtcgcggc ggtcccggcg tacttctcct cccgcatcaa
     1141 agtgatgagc gcggagcacg aggcgctcgc cgtggagctg cggcaggcgc ggctcgaagc
     1201 gagcgacgtg ctccacaacc tggcgagcgg cgtgctcacc gtggatgcag acggcaagct
     1261 cctgttcgcc aacccggccg ccgagcagat tctggggttc cgcgcgaagg actggctgga
     1321 ccgcgccttc ctgccggagc tggcgcgcac cagccccgag ttctgggcgg cggtcgcggc
     1381 caccgcgcgc cggggcatcc ggctgatgcg ggtcgaggcg accgtgcggc gcggcgagcg
     1441 cagcttcccg gtcggtctca cgaccaccac gatcgccggg aacgccgggt tgccgccgcg
     1501 cgtgacggcc atcttcaccg atatccccga ctccaagcgc ctcgaggagc tgcaccttcg
     1561 tgccgaacgg ctcgaagccg tggccgagct ctccgccggg ctggcccacg agatcaagaa
     1621 cccgctcgcc tcgatccggt cgtcggtgga gcagctgtcc cggtccaccc ggacgaatcc
     1681 ggacgagcgg tttctcgcga gcctgatcgt ccgcgagagc gaccgtctca gcaggctgct
     1741 ctcggagttc ctggatttct cccgtgtccg ggtcaccgaa taccgggcgg tcgatctgcg
     1801 ggacgtggcg ggggcggcgg tccgcctggt gcgccagcat ccggactgtc cagccgacgt
     1861 gcagatcgaa ttcgtcggtg gccctacgcc catggagggc gacgaggacc tgctgcaccg
     1921 cgtggtctcc aacctggtgc tcaacgccgt ccaggcgagc ggtccggggg tgcacgtccg
     1981 cgtgttcacg gggcggccga agccggagga ggtcccggcc ggcttcacca tggagaatcc
     2041 ggtggccctg cgggtgagcg acaacggtcc ggggattccg gaggcggtgc gcgagcggtt
     2101 gttcgagccg ttcgtcacgg gacgacaggg cgggaccgga ctcggtctcg ccatcgtgca
     2161 gcgcgccgtc gccgcccacc ggggcatggt cctggtggac accacgccgg gacacggcac
     2221 gacgttcacg gtgtttttcc cggccgcgcg ccggaaggaa gaagccgcat gacgggcagc
     2281 cccgccgctc cgacgctact gatcgtcgat gacgagtcgg ccattctcga cgcgctccgc
     2341 atcctgctca agaacgaggg cttcgacgtg ctcacggccc agggcgggaa ggcgggcctg
     2401 gagcagctcc gcaacgcggc gccggacatc gtgctcaccg acgtgcgcat gccgctggtc
     2461 acgggcctcg acatcctcac ggccgtccgc cagcaggatc cggagacgcc ggtcatcctc
     2521 atgaccgccc aggcctcgct gcagacggcc atccaggcgg tgaacgaggg cgccttctat
     2581 tacatccaga agccgttctc caacgacgac ctggtcgcga tctgtcgccg tgccgccgag
     2641 caccggcggc tgcgggcgga gaacaagcag ctgaaacagg agattcgacg gcgcgagcgt
     2701 tcgcgcttcg cgaagcacct cggcaggtcg cgcttgttcg ccgacgtgct gcgcctggcc
     2761 gagcaggtgg cgcccaccga gagcacggtg ctgatccagg gcgagtccgg caccggcaag
     2821 gaggtcattg cccgctacat ccacgacctg tcctcgcgca acgaggggcc gttcctgtcg
     2881 ctgaactgcg gggccctccc ggagagcctg ctcgaatcgg agctgttcgg acacgtgaag
     2941 gggtccttca ccggggccgt gcgggacaag caggggctgt tcgcggccgc gcgcggcggc
     3001 acgttcttcc tggacgagat cggcgagatg tcgcccgcca cccaggtcaa gctgctccgc
     3061 gtcttgcagg agcgggaggc gatccccgtc ggcggcaccg aggcgatccc cgtggacgtg
     3121 cgggtcgtgg cggcgaccaa ccgcgacctg gaggacgaga tccggcaggg ccggttccgc
     3181 accgatctgt actaccgcct caacgtgatc gccatccacc ttccgccgct gcgcgagcgc
     3241 cgcgacgaca tcgagatctt cgtcaccgcc ttcctcgagc gcatcgccaa ggagaagagc
     3301 accccggtga agcacctgat gccggaggcg gcggaggcgg tgttgagcta cgactggccc
     3361 gggaacgtgc gggagctgga gaacgccctc gaacgtgccg tcgtgttgac gaagggagaa
     3421 gcgatcccgc tctcggccat gcccgccaag gtcaccgagc gtcgcgtcga gccgctggtc
     3481 gccgaccgcg tgcaccacaa cccgacgctc gacaccatcg agcgggccta catcgcctgg
     3541 gtcctccagg cggaaggcgg gaacaagagc cgggcggcgg aagtgctggg catcgatccc
     3601 tcgaccctgt atcgcaagct gagccggtac gagggaggga gtgatggccc gatctgaggc
     3661 ggccgatccc gtggggatcg agacgctcga gcgcctggcc gccgccgctc ccaactacaa
     3721 tcgctggctg ttcgagcggg tgggacggtg ggtgggacgc agggtgctgg agatcggtgc
     3781 cggagtcggg acgatgtcgg cattcctcat gaacggtgcc cgcgagcggg tggtgctgtc
     3841 ggacaccgat gcgcattacc tcgagttgct gcgtgagcgg tacgcgcgtc atccctacgt
     3901 gggagtcgcc cggctccgcc tcccgactta cgaggaagcg ctcgcccgcg agagcttcga
     3961 cactatcatc tgcctcaacg tgctggagca cgtgcgcgac gacctgctct ccctcggcac
     4021 catgttccgg ctgctgcaac ccggcgggcg gctggtgctg ctcgtaccgg cgctgcccgc
     4081 gatctacggg gagctggacc gggccctggg ccactttcgg cgctaccggc ccggagacct
     4141 ccggcgcagg tacgccgccg ccgggttcgt catgcgccac ctcgagtact tcaacctggc
     4201 cggcatcccg ggctggtggc tcacggggcg ggtgctgaag cgcggcctga ttcccaccgc
     4261 gtcgttgcgg tggtacgacg cgctcgtgcc gctgtttcgg ctcgagcgcc tgttgccgtg
     4321 gcggatcggg caatccctca tcgccgtcgg cgagcggccg tcgtgaccgg tcccgcggcg
     4381 gagggagcgc cggtgctgtc ggtggtcgtg ccggcgttca acgaggcgac gacggtcgaa
     4441 accgtggtcc gtcgtgtgcg cgacgtgccg ctccgcctgg aggtcatcgc cgtcgacgat
     4501 gcctccgccg acggcacggg cgacgtcctc gaccgcctcc accgggaagg actgctcgac
     4561 cgcgtgatcc atcattccgt gaaccgcggg aagggcgcgg cactccggtc cgggtttgcc
     4621 gtcgtgacgg gcgacgtggt ggtcgtgcag gacgccgacc tggaatacga cccgtcggac
     4681 cttccggcgc tgctggagcc gatccgccgc ggccgtgccg atgccgtgta cgggtcacgc
     4741 ttccagggag ggccccaccg cgtgttgttc ttctggcacg ccgcgggcaa cctgattctc
     4801 accctgctgt ccaacatgct caccaatctg aacctgtcgg acatggaaac gtgctacaag
     4861 gtggtgcggc gggacctgct ccaacgcctc cccctcacct ccgaccgctt cggattcgag
     4921 gtcgagctga cggcccggct ggcgcaagcg ggggcccgca tctgggagct gccgatctcc
     4981 tacagcggcc gtacctacgc cgaggggaag aagatcacct ggcgcgacgg gttcgcggcc
     5041 ctgctgcata tcctgcgctt taatctgttt ccaccgggcg gagactggcg tgagccgcgg
     5101 cgggccgatg gcgctcctcg ctgagcgcct gagtggacgc cgcctctacc tggtggtggg
     5161 ggcggtcgcc ctcgtcgtgt atctcggggc gctggggaac cggttcgcca tggacgacct
     5221 tcccctggtg gtccggaatc cgctggtcca gagcgtgtcg ggcatgtggc aggccttcgc
     5281 cgccccgttc tggccgcccg acttcggcgg tcacatgtat cgtcccctcg cggtggccac
     5341 ctgggccctc gaccagcggg tggacggcgc ccggtggttc catctcgtca acctgttgtg
     5401 gcacgccgcg gcttgcgtc
//
LOCUS       DBNGZW010000403         2748 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_516749, whole genome shotgun sequence.
ACCESSION   DBNGZW010000403 DBNGZW010000000
VERSION     DBNGZW010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_516749"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="AC16JC_09510"
     CDS             <1..399
                     /locus_tag="AC16JC_09510"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5164969.1"
                     /translation="KVADIMQTTVETIGPDDTVADAVTTIAEQHVSGLPVVDQRRHLI
                     GVISSTDVLQLLSEVTDPERRGNILESTTVREIMTAKPVTVDPDADVHDAARQMLYGE
                     IHRLFVEYDGELVGVVTQSDIVGVVAGAKL"
     gene            408..809
                     /locus_tag="AC16JC_09515"
     CDS             408..809
                     /locus_tag="AC16JC_09515"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5164970.1"
                     /translation="MRVADIMRTNLKTIDAEASVADAVAALTEYGVSALPVLDRHGRA
                     VGVCSTRDIIAAEARCHDPVAREQLFEKTLVLEVMTPWPATAHPDEDVRKVAKRLLEE
                     KVQRLFVEEKGALVGVVSQTDIVAAVAATRV"
     gene            complement(828..1577)
                     /locus_tag="AC16JC_09520"
     CDS             complement(828..1577)
                     /locus_tag="AC16JC_09520"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5164971.1"
                     /translation="MSQPYDPRVTIQGDGAPLVLVPGMDGTGRLFYRQVPPLARRYRV
                     ATYALRDDAAGMDVLVEDLAQVVAAASGPGSAIVIGESFGGTVALSFALAHPERVRAL
                     VVLNSFSRFLPQVNLQLAIAALRLLPWETMPLVRRLTAFRLHSRYTHRAEVRRFLEVT
                     RETRRDGYIARLRILQQYDAREHLGALRMPTLFLASELDHLIPSVREGRYMAERVPGA
                     TLRVLRGHGHICLIAPNLDLEQVLRDWRPDL"
     gene            1576..2310
                     /locus_tag="AC16JC_09525"
     CDS             1576..2310
                     /locus_tag="AC16JC_09525"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR5164972.1"
                     /translation="MYTLDKLGRLGWAIFSAFAVESVVLGLSVLPAVLFWEWTFRWPL
                     PANWIRIVLLSMSLVPAYLLFSVALMTLTAVAVRLAGWRTPPMLEMRIVDLEWPLLDW
                     ARYGMTTHVVRVFAGTLLRATPLWTLYLRLNGARLGRHVFVNSVTLSDHNLLECGDDV
                     VIGAGVYLSGHTVERGVVKTAPVRLGSGVTIGVGSVIGIGVSIGARTQVGALSVVPKH
                     RALEPDAVYGGVPARRLDVPDVIEGT"
     gene            complement(2307..>2748)
                     /locus_tag="AC16JC_09530"
     CDS             complement(2307..>2748)
                     /locus_tag="AC16JC_09530"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5164973.1"
                     /translation="RSRVPVLVAPAQHRAYRRVLACVDDSPHAVEVFEVAQAAAEAFA
                     AHLVALHVEVAEEGALGQGERRAWLRYVEQGGSGGAAVANCETIVRQGDVTTEILAEC
                     RAEDADLLVFGYRRGMNYGDPGAVTTVAARLLRRAACALVAVPV"
BASE COUNT          423 a          979 c          944 g          402 t
ORIGIN      
        1 aaagtcgccg acatcatgca aacgaccgtc gagacgatcg gacccgacga caccgtggcg
       61 gacgccgtca ccaccatcgc cgagcagcac gtgtccggcc tgccggtggt ggaccagcgc
      121 cgtcacctga tcggtgtgat ttcgtccacc gacgtgctgc agctcctgtc ggaagtaacc
      181 gacccggagc gccgcggcaa cattctggag tcgacgaccg tgcgcgagat catgacggcc
      241 aagccggtca cggtcgaccc cgacgccgac gtgcacgatg cggcgcggca gatgctgtat
      301 ggcgagatcc accggctgtt cgtcgagtac gacggggagc tggtcggcgt cgtgacgcag
      361 tccgacatcg tcggcgtggt cgcgggcgcc aagctctagg aggacgtatg cgagtcgccg
      421 atatcatgcg caccaacctg aagaccatcg acgccgaggc ctcggtggcc gacgcggttg
      481 cggcgttgac cgaatacggc gtgtccgccc tgccggtcct cgaccgccat ggccgcgccg
      541 tgggcgtctg ctccacccgg gacatcatcg cggccgaggc acgctgtcac gacccggtcg
      601 cgcgggagca gctgttcgag aagaccctgg tgctcgaagt catgacccca tggccggcca
      661 ccgcgcaccc ggatgaagac gtgcgcaagg tggcgaagcg cctgctggag gagaaggtcc
      721 agcggctgtt cgtggaggag aagggcgcgc tggtcggcgt cgtctcccag acggacatcg
      781 tcgcggcggt ggcggccacc cgcgtctagc gggccgaccg cctcgcctca gagatccggg
      841 cgccagtcgc ggagcacctg ctccagatcc aggttcgggg cgatgagaca gatgtggccg
      901 tgcccgcgca gcacccgcag cgtcgccccg ggcacccgct cggccatgta gcgcccctct
      961 cgcaccgagg gaatcagatg gtcgagctcc gacgccagaa acagggtcgg catccgcagc
     1021 gcgcccaggt gctcgcgggc atcgtattgc tgcaggatgc gcagccgcgc gatatagccg
     1081 tcgcgccggg tctcccgggt cacctcgaga aaccgtcgga cttccgcccg atgcgtgtag
     1141 cgcgaatgca ggcggaacgc ggtcaggcgg cgcaccagcg gcatcgtctc ccagggcagg
     1201 agccgcagcg cggcgatggc cagctgcagg ttgacctgcg gcagaaaccg ggagaaggaa
     1261 tttagcacga ccagcgcccg cacgcgctcg gggtgcgcca gcgcgaaact cagagcgacc
     1321 gtcccaccga acgattcgcc gatcacgatc gcggacccgg gaccgctcgc cgccgcgacc
     1381 acctgggcca gatcctcgac cagcacgtcc atgccggcgg cgtcatcgcg cagcgcgtag
     1441 gtcgccacgc ggtagcgcct ggcgagcggc gggacctggc ggtagaacag ccggcccgtc
     1501 ccgtccatcc cggggacgag cacgagcggc gccccgtcgc cctggatggt cacccgcgga
     1561 tcgtatggct gggacatgta cacgctagat aagctcggcc ggctcggctg ggcaatcttc
     1621 tccgcgttcg cggtggagag cgtcgtcctg ggactgtcgg tgctgccggc cgtgctgttc
     1681 tgggagtgga ccttccgctg gccgctgccc gcgaactgga tccgcatcgt gctgctgtcg
     1741 atgtcgctgg ttcccgccta tctactgttc tccgtcgcgc tgatgacgct cacggcggtc
     1801 gcggtacgac tggccggctg gcgcacccca cccatgctcg aaatgcgcat cgtggacctg
     1861 gagtggcccc tgctcgactg ggcgcgctac ggcatgacca cccacgtcgt gcgcgtgttc
     1921 gcgggcacgc tgctgcgcgc cacgccgctg tggaccctct acctgcgatt gaacggcgcc
     1981 cggctcggcc ggcacgtctt cgtcaacagt gtcacgctgt cggaccacaa cctgctcgag
     2041 tgcggagacg acgtggtgat cggggcgggc gtctatctct cggggcacac cgtggagcgc
     2101 ggcgtcgtca agaccgcccc cgtgcggctg ggatcgggcg tgacgatcgg ggtcgggagc
     2161 gtcatcggca tcggcgtgag catcggggcg cgcacccagg tgggcgcgtt gagcgtcgtg
     2221 cccaaacacc gcgcgctgga gcccgatgcc gtgtacggcg gcgtcccggc gcgacgtctc
     2281 gacgtgcccg atgtgatcga agggacttag accggcaccg cgaccagcgc gcaggcggcg
     2341 cggcgcaaca gtcgtgcggc gaccgtcgtg accgccccgg ggtcgccgta gttcatcccg
     2401 cgccggtagc cgaacacgag gagatccgcg tcctccgccc ggcactccgc gaggatttcc
     2461 gttgtcacgt caccctgccg cacgatcgtc tcgcagttcg cgaccgccgc cccgccgctg
     2521 cctccctgct cgacgtaccg gagccaggcc cgccgctctc cctgccccag cgcaccctcc
     2581 tccgccacct ccacgtgcag ggccacgaga tgcgccgcga aggcttctgc ggcggcctgc
     2641 gcgacctcga acacctccac ggcatgcggg ctgtcgtcca cgcacgccag cacgcgccgg
     2701 tatgcgcgat gctgggccgg cgccaccagc accggcacgc ggctgcga
//
LOCUS       DBNGZW010000404         6296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1538215, whole genome shotgun sequence.
ACCESSION   DBNGZW010000404 DBNGZW010000000
VERSION     DBNGZW010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6296
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1538215"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..120)
                     /locus_tag="AC16JC_09535"
     CDS             complement(<1..120)
                     /locus_tag="AC16JC_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5164974.1"
                     /translation="MQKITPFLWFNGDAEAAAEFYVSIFKNSKVLSVSGPKGKA"
     gene            complement(156..656)
                     /locus_tag="AC16JC_09540"
     CDS             complement(156..656)
                     /locus_tag="AC16JC_09540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010569000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5164975.1"
                     /translation="MRHAGTLQVKAIGEREIAITRDFDAPRRLVFDAYTKPELVRRWL
                     LGPDGWSMPVCEIDLRVGGAYRYVWRHLTNGAEMGMGGVYREIAAPDRLVATEKFDEA
                     WYPGEALNTLVLVERAGRTTLTQTMRYESREAREAVLKSPMEQGLAVSFDRLEALLTS
                     TPGGVA"
     gene            complement(670..1038)
                     /locus_tag="AC16JC_09545"
     CDS             complement(670..1038)
                     /locus_tag="AC16JC_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010412310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5164976.1"
                     /translation="MVMRASAPARLDATFAALADPTRRAILTRLAQGEASVSELAEPF
                     AMSQPAISKHLKVLERAGLISQGRDAQRRPRRLEARPLAEATAWLERYRRFWEGRFRQ
                     LDVLLDELQATRPPRKSTER"
     gene            complement(1134..1316)
                     /locus_tag="AC16JC_09550"
     CDS             complement(1134..1316)
                     /locus_tag="AC16JC_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR5164977.1"
                     /translation="MLDYVVTLTQDATRIGPAHHDRLRAAGFDDRAILQITLIAAWFN
                     YINRVADALGVGRDAP"
     gene            complement(1425..1706)
                     /locus_tag="AC16JC_09555"
     CDS             complement(1425..1706)
                     /locus_tag="AC16JC_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR5164978.1"
                     /translation="MTWIRTIPAQEADDKLRAALEAQKALYPTEYATPVQPTADGTSG
                     IVASHSLIPDALYHAFATFGVLMSPELPLSRRQHEMITTMVSVTNRCRY"
     gene            complement(1703..3058)
                     /locus_tag="AC16JC_09560"
     CDS             complement(1703..3058)
                     /locus_tag="AC16JC_09560"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5164979.1"
                     /translation="MHDTTGNRAALTRQLGLVSVTALVIGEVVGVGIFLTPASMARSL
                     GSPFWLLVVWLLMGAMALSGALCYGELAARFPEAGGGYVYLRRTYGPAVAFLYGWKCL
                     LVMDPGITAALAMGLASYVGYVVPLSDVGLKIVAIGVILALAAVSIAGVRFSAGLMRW
                     LTAFKLAALSLIVVLAIGRGLGNWSNFVPFVAQRPGSDPLPGALAPGLVGAFFAFGGW
                     WEITKLTGEARDPARTLPRALASGVAVVTAVYVLTSAVFLYLIPLERVTSGETFAAQA
                     GEALFGRAGGQVFAGIVIVAVAGSLLALLMALPRVYYAMARDGVFFEAVAAIHPRLAT
                     PARAIGLQAALASLLVLLGTFSQILSYFIFVTVLFVALTVAALFVLRHRASAPPAFET
                     PGYPVTPLVFLLLVAVLLLLLVGHDPAQALLGVAIVGLGFPVYLFAFRGRLTPPLDDT
                     T"
     gene            3365..3904
                     /locus_tag="AC16JC_09565"
     CDS             3365..3904
                     /locus_tag="AC16JC_09565"
                     /inference="COORDINATES: protein motif:HMM:NF028519.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HIR5164980.1"
                     /translation="MRVHVVLFALVTTPFLAGAAQGAAVKDPATCAVADSNRSATSWS
                     KDRPLDPFGRPRTGCAPVAPAPQSGGGGDPGGGTGGQTGTPVISGTVWNGSSWLGMGG
                     WVIEVTGTATASAVTNADGTYGFWNLPPGTYTVCEQVKAGWTQTYPTSGSPCPTGQGY
                     TFTLTSGGSGSFVDFVDIQ"
     gene            4166..>6296
                     /locus_tag="AC16JC_09570"
     CDS             4166..>6296
                     /locus_tag="AC16JC_09570"
                     /inference="COORDINATES: protein motif:HMM:NF018190.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HIR5164981.1"
                     /translation="MLFLAAGLALPVGLAAQTAVTRSDHACLQVAPTAVPVHFDGRLD
                     GPAWDGADSITEFRQREPAEGAPASERTVVKMIRDAAALYIGVRAYDDDPLRVRASEL
                     RRDADLSSDDNVVLLIDSFHDHRGAFVFGTNPRGAMWDAQLVDLDDLNEDWNGIWDVV
                     VTRDDSGWTAEFRIPFRTLRFRGATDLRFGFNVRRFIRRTNEEDLWRSWGRAEGLYQL
                     LNEGELQSLGGLRRPRDLEVYPYALTRAIESEHDSTGARVASGFFGGKAGVDAKVTVS
                     PTLTADLTAATDFAQVEVDQQVINLTRFPFFFPEKRAFFLESSGLFDFGTPGRVQVLY
                     SRRVGLDSTGGVVPIVAGGRVYGRTGPWRFGVLDARTGGADRANNAVVRVQRDLLARS
                     YVGAIGSLRTVAGAGPEAAGGVDLDLPLVIHGQNVEPKLWVAGTRTPGVAGFPLAWRL
                     STDNPNDLFDNFVSLYKIDAGFDPALGFVRRVGIWETTGHIDFMPRPHLLGIRQLDFE
                     IPSWDVIANQSGSLGRTGDWQTAEFEWRLFGGDRQSGDRFEINVQRLMDAPPDSFEIF
                     YDVKIPPGRYWWSRYELQYLMAPGRPLSFGAVVNWGRFYGGRSTDMELQGTWRGGGHV
                     ILGAEVTRSSARLPGGKFTALQTSEHVEYDFNTRTSFLGFVQYTNDDQRVDFNLRFHW
                     IPAIGDDVFLVWNSGYTTNHLATYRFPA"
BASE COUNT         1024 a         2138 c         2151 g          983 t
ORIGIN      
        1 ggccttcccc ttcgggcccg acacgctcag caccttcgag ttcttgaaga tcgagacgta
       61 gaactccgcc gccgcttcgg cgtcgccgtt gaaccacagg aacggggtga tcttctgcat
      121 tggagtctcc taggtgtgat tcggagtttc ggtggttacg cgaccccgcc cggggtcgac
      181 gtcaacagcg cttccagccg gtcgaagctc acggcgaggc cctgctccat gggagacttg
      241 agcacggcct cgcgcgcctc gcgcgactcg taccgcatcg tctgcgtgag cgtcgtccgc
      301 cccgcccgct ccacgagcac cagcgtgttc agcgcttcgc ccggatacca cgcctcgtcg
      361 aacttctccg tggcgaccag gcgatccggc gccgcgatct cgcgatacac gccgcccatg
      421 cccatctcgg cgccgttcgt gaggtgccgc cacacgtagc ggtaagcgcc cccgacccgc
      481 aggtcgatct cgcataccgg catcgaccag ccgtcgggtc cgagcagcca gcggcggacg
      541 agctcgggct tggtgtatgc gtcgaatacc aggcggcgtg gcgcgtcgaa gtcgcgcgtg
      601 atcgcgatct cccgttcccc gatcgctttg acctgcagcg ttccggcgtg tctcatcggt
      661 cgatctccct tatctctcgg tggatttgcg gggtggccgc gtggcctgca gctcgtcgag
      721 cagcacgtcg agctgccgga accggccttc ccagaagcgg cgatagcgct cgagccaggc
      781 ggtcgcctcc gcgagcggcc gggcctcgag ccggcgcggg cgccgttgcg cgtcgcgtcc
      841 ctgcgagatc aggccggcgc gctccagcac cttgaggtgc ttggagatgg cgggctggct
      901 catggcgaac ggctcggcca gctcgctgac cgaggcctcg ccctgcgcca ggcgcgtcag
      961 gatcgcccgc cgcgtgggat cggcgagggc cgcgaacgtc gcatcgaggc gcgcgggagc
     1021 ggaggctctc atgaccatga ccggtccccc tgtgaataac tgttcagttc cacaaccata
     1081 tggtaatcta ctgagcgccc ggctgcctgt caagcagccc gcacggctcg cggctaggga
     1141 gcgtcgcggc ccacgcccag cgcgtcggcc acccggttga tgtagttgaa ccaggcggcg
     1201 atcagcgtga tctgcaggat ggcgcggtcg tcgaatcccg ccgcgcgcag gcgatcgtgg
     1261 tgggcgggcc cgatgcgggt ggcgtcctgc gtcagcgtca ccacgtagtc cagcatgacc
     1321 cggtccgccg gcgcgacggg ggcggtggtg tagtcgtgct ccagcgccgc taccagcgct
     1381 ggatccagcg tgacccgacg cagaaactct gcgtgagagg tgattcagta gcgacagcgg
     1441 ttggtgaccg acaccatcgt cgtgatcatc tcgtgttgcc gccgcgagag cgggagctcg
     1501 ggcgacatga gcacgccgaa ggtggcgaac gcatggtaca gggcatccgg gatgaggctg
     1561 tgcgaggcga cgatgcccga cgtcccgtcc gcggtcggtt gcaccggggt cgcgtactcc
     1621 gtggggtaca gcgccttctg ggcctccagg gccgcccgca gcttgtcgtc cgcttcctgc
     1681 gccgggatgg tacgaatcca ggtcatgtcg tgtcgtccaa tggaggggtg agccgcccgc
     1741 gaaacgcgaa caggtaaacc ggaaacccga gtcccacgat ggcgacgccg agcagcgcct
     1801 gcgccggatc gtggcccacg agcaggagca gcaagaccgc aacgaggagc aggaagacca
     1861 gcggcgttac ggggtagccc ggcgtctcaa aggccggagg cgcgctcgcc cggtggcgca
     1921 acacgaacag cgcggccacg gtgagggcca cgaacaacac cgtgacgaaa atgaaatacg
     1981 acaggatttg actgaacgta cccaacagga ccagcagcga cgccagggcg gcctgcagcc
     2041 cgatggcccg cgccggcgtg gcgagtcgcg ggtggatcgc ggccaccgcc tcgaagaaca
     2101 cgccgtcccg cgccatggcg taatacaccc gggggagggc catcagcagg gcgaggaggc
     2161 tcccggccac ggcgacgatg acgatgccgg cgaacacctg gccccccgcg cgcccgaaca
     2221 gcgcctcgcc cgcttgcgcg gcgaacgtct cgccggaggt cacgcgctcg agcggaatca
     2281 gatagaggaa caccgcgctc gtcagcacgt agaccgccgt gacgacggcc acgccgctgg
     2341 ccagggcccg cggtagggtg cgcgcggggt cgcgggcttc tccggtgagc ttggtgatct
     2401 cccaccatcc gccgaacgcg aagaacgccc ccaccaggcc gggagcgagc gcgcccggca
     2461 gcggatccga tccgggacgc tgcgccacga acggcacgaa gttcgaccag ttccccaggc
     2521 cccggccgat cgccagcacg acgatgaggc tcagcgcggc gagcttgaag gcggtgagcc
     2581 agcgcatcag gccggcgctg aaccggacgc cggcgatgct caccgccgcg agcgccagaa
     2641 tcaccccgat cgccacgatc ttgagcccca cgtcggagag gggcaccacg taccccacgt
     2701 agctcgcgag gcccatcgcc agcgccgcgg tgatccccgg atccatcacg agcaggcatt
     2761 tccatccata caggaacgcc acggcgggcc cgtacgtgcg gcgcaggtac acgtacccac
     2821 cgccggcttc gggaaagcgg gcggccagct cgccgtaaca cagcgctccg ctgagcgcca
     2881 tcgcgcccat gaggagccag accacgagca gccagaaggg cgagcccagc gaccgagcca
     2941 tgctcgccgg ggtgaggaag atcccgacgc cgaccacctc gccgatcacg agggcggtga
     3001 ccgagacgag gccaagctgg cgggtcaggg cggcgcgatt gccggtcgtg tcgtgcacgg
     3061 gcgggctaag ctccgcccgt ggacccggga gcgcaagacg ggctcccggc agggaagcgg
     3121 gactgccgga attcgagagt gccggactcc tcggcaagta acgtttggca cggcggcacg
     3181 ccgcgtgagt cgcgtcacag gcccacaggt cgcatcgcgg agtcgcggtt tcgccgctgc
     3241 gtggccgtgc gcaagtattg gacgcatcgc gtgttcctcc gccgcgtgat gtgcgacacg
     3301 gctccacgtc cgccactgtg cgattgtgtc ccgcgcctga tcgcgtacct aacaggagag
     3361 caagatgcgt gtgcatgtag ttcttttcgc gctggtcacg acaccgttcc tcgcgggagc
     3421 ggcgcaaggc gctgccgtca aggaccccgc gacctgcgcg gtggccgact cgaaccgctc
     3481 ggcgacctcg tggtcgaagg accgtccgct cgatcccttc ggtcgtcccc ggaccggatg
     3541 cgcccccgtg gcacccgcac cccagtcggg tggaggtgga gacccgggcg gcggaaccgg
     3601 gggccagacc ggcacacccg tgatctcggg gacggtctgg aacggcagtt cctggttggg
     3661 gatgggggga tgggtcatcg aagtcaccgg cacggcgacg gcgtcggcgg tcaccaatgc
     3721 cgacgggacg tatggattct ggaatctgcc cccgggcacc tacaccgtct gcgagcaggt
     3781 gaaggcgggc tggacgcaga cctatccgac gagcggatcg ccctgtccga ccggtcaagg
     3841 gtatacgttc accctcacca gcggcggaag cggcagcttc gtcgacttcg tggacatcca
     3901 gtagcagacg cttctcgctc gatcaggcgg gaacgaaacc gagtcccgca tccgccgacg
     3961 gcgggtgcgg gactcttctt cctggagcaa tcccagcagg cgagcgagaa gtcgccgtcc
     4021 cgccgcgggt tcaccgctgc cccgggtcgc ggcgcgaacg cctgcgggaa gtgattcgct
     4081 tgctgccggg atacaacgcc aatacacttg tacgcaggtg gccgacctgc gtcactccca
     4141 tcgatctgcg gcgggccgca gccgcatgct gttccttgcc gccggcctgg ccctccccgt
     4201 cgggcttgcc gcccagaccg cggtcacgcg gtctgatcac gcgtgccttc aggtcgcccc
     4261 cacggctgtt cccgtgcatt tcgatgggcg gctggatggg ccggcgtggg acggcgcgga
     4321 ctcgatcacc gaattccgcc aacgcgagcc ggccgaaggg gcccccgcga gcgaacgcac
     4381 cgtcgtcaag atgattcggg atgctgcggc gctctacatc ggagtgcggg cgtatgacga
     4441 cgacccgctc cgcgtacgcg ccagcgaatt gcgccgcgat gcggaccttt cgagcgacga
     4501 taacgtcgtg ctcctgatcg acagcttcca cgatcaccgc ggcgcattcg tgttcggcac
     4561 gaacccgcgt ggcgccatgt gggacgccca gctcgtcgac ctcgacgact tgaacgagga
     4621 ctggaacgga atctgggacg tggtggtaac ccgtgacgac tcggggtgga ccgccgagtt
     4681 ccggatcccg tttcgcacgc tccggtttcg tggcgccacc gatctccgct tcggcttcaa
     4741 cgtccgtcgc ttcatccgtc gcacgaacga agaggactta tggcggtcct ggggccgcgc
     4801 cgaggggctg tatcagctgc tcaacgaggg ggagctccag agcctcggcg ggctgcgtcg
     4861 tccccgggac ctcgaggtct acccctatgc cctcacccgc gccatcgagt cggagcacga
     4921 cagcacgggc gcacgggtgg cgagcgggtt cttcgggggg aaggccggtg tcgacgcgaa
     4981 ggtgaccgtc tcgccgacgc tcacggccga tctcaccgcg gccaccgact tcgcacaggt
     5041 tgaggtcgat cagcaggtca tcaacctcac ccggttcccg ttcttctttc cggaaaagcg
     5101 agcgttcttc ctcgagtcga gcggcctgtt tgacttcggg acgccggggc gagtccaggt
     5161 gctctattcc cgtcgcgtcg gactcgactc cacgggcggc gtcgtaccca tcgtcgctgg
     5221 aggtcgggtg tacgggcgca ccggcccctg gcgtttcggc gtgctcgatg cccgcacggg
     5281 cggcgccgat cgcgccaaca acgcggtcgt gcgggtgcag cgcgacctgc tcgcgcgttc
     5341 ctacgtcggt gcgatcggca gcctgcggac ggtcgccggc gccggcccgg aggcggcggg
     5401 gggggtggac ctggacctgc ccctggtgat ccatggacag aacgtcgaac cgaagctctg
     5461 ggtcgccggc acccggacgc cgggggtcgc cgggttcccg ctcgcgtggc ggctgtccac
     5521 cgacaacccc aacgacctgt tcgacaactt cgtctcgctc tacaagatcg acgccggctt
     5581 cgatccggca ctcgggttcg tgcggcgcgt ggggatctgg gaaaccaccg gacacatcga
     5641 cttcatgcca cggccgcacc tgctgggcat ccgccagctg gatttcgaga tcccgagttg
     5701 ggatgtcatc gccaatcagt ccggatcgct ggggcgcacc ggtgactggc agacggccga
     5761 gttcgagtgg cgactcttcg gtggggacag gcaatccggt gatcgcttcg agatcaatgt
     5821 gcagcgactg atggacgccc cgccggattc gttcgagatc ttctacgacg tgaagatccc
     5881 gcccggccgc tactggtggt cacgctacga gctgcagtac ctcatggctc cgggtcgacc
     5941 cctgagtttc ggggcggtcg tcaactgggg acgcttctac ggtggccgca gcaccgacat
     6001 ggagctccag gggacctggc ggggtggggg acatgtcatt ctcggcgcgg aggtgacccg
     6061 ctcgtcagca cgcctgcccg gggggaagtt caccgcgctc cagacctcgg agcatgtgga
     6121 gtacgacttc aatacccgta cgagtttcct gggcttcgtg cagtacacaa acgacgatca
     6181 gcgggtggat ttcaacctcc gattccattg gatcccggcg atcggcgatg acgtcttcct
     6241 ggtctggaac tcggggtata cgaccaacca cctggcgacc taccgcttcc cggcgg
//
LOCUS       DBNGZW010000405         4686 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1606859, whole genome shotgun sequence.
ACCESSION   DBNGZW010000405 DBNGZW010000000
VERSION     DBNGZW010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4686)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4686)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4686
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1606859"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1111
                     /locus_tag="AC16JC_09575"
     CDS             83..1111
                     /locus_tag="AC16JC_09575"
                     /inference="COORDINATES: protein motif:HMM:NF024921.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR5164982.1"
                     /translation="MALLALAVIGCSGAGKTPLVIYTPHGRDLLTLLKARYERLHPEV
                     DLRWLDMGSQEVYDRVRSERANPQGDVWFGGPASIFALGAGDSLLEPYHPAWADHISP
                     GGRGPADLYFSAYETPAVIVYAEQALTPAQAPQDWDDLLDAKWKGKILIRDPIASGTM
                     RAVWGMIIERGLKQTGDTAAGFDWLRRLDAQTKEYVLNGPLLDQKIVRQEGLVTIWDL
                     PDIQLNKRDGLPLGYVFPTSGTPAIEDAIGIIRNAPHRDAARAFVEWVGSTEAQLVAA
                     REAYRLPAREDLPVDSLPEWVRDVRRRLVTADMDWDLLARQGGAWMRYWDENVGGKGE
                     GGRGKGGR"
     gene            1101..2114
                     /locus_tag="AC16JC_09580"
     CDS             1101..2114
                     /locus_tag="AC16JC_09580"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5164983.1"
                     /translation="MGVEEAFLSLDELRVRFSDTVTIGPVSLSIARGEILALLGPSGS
                     GKTTLLRMLAGFERPDGGTVRIEGEDVTAVPPARRRVGMVFQHYALFPHLDVGENVAF
                     GLHGTRDEGRGTSAVVAEALALVDLAGFERRKVQELSGGQQQRVALARALAPAPRVLA
                     LDEPLSNLDPSLRERTRRELRQLIKRIGITAVFVTHEQDEAFDLGDRIAVLSAGRLNQ
                     VGTPEDLYERPANLFVGTFVGRANVLRGATARALGAADGEAILVRPEQLRFGAGEGER
                     GGLNGVVAERRYTGAAAFFQVDLETGERVEVEAEPQAARVGDRVRIGAVRTLRFAGPQ
                     GEG"
     gene            2111..3754
                     /locus_tag="AC16JC_09585"
     CDS             2111..3754
                     /locus_tag="AC16JC_09585"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5164984.1"
                     /translation="MTRSRRAGGGRSGPAGVALTLLLAWLVLYPIVLLVVDGLHGDAL
                     RTFVERSGEWRALWASVWISLASVVLAAVIGVPLAFLFEWFEFPGRKLLGSLIALPAV
                     LPPFVGVIAFLFLYGESGFAARFLQFVFRFQQAPWHLQGAGAILLVHAYSMYVYFYLF
                     ARAGLARLDAATLEAAQALGAGRWRTITRVVLPLIRPSLAGAALLTFMTSLGSFSAPY
                     VFGGGFRVMTTQIFASKQNGELGVAMVETVALALVALAGLALLRRTEGDAILAGLGKG
                     TAPRRRALRRPLVRAATTLVGWGLAVLLLLPHATLVLVSLVPYATWTTETLPPVLSLV
                     NYGRLFAEPERLEPLVNSFWMAAVSTSLAVAVGLAAGWLVVRRRVALRRWIEGLLALP
                     WALPGTVFAIALATAFSVSAPLQLRWILVGTVAILPLAYFVRNLPVTGRAILAGYRQL
                     DPALEEAAASLGAGPWRTLRRVTLPQLRPALAAGASLAFVGALGDFVTSILLYTYRNR
                     PISMEINAALRVNEIGVAAAFGVVLMVVSAVVLSIGARR"
     gene            3751..>4686
                     /locus_tag="AC16JC_09590"
     CDS             3751..>4686
                     /locus_tag="AC16JC_09590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5164985.1"
                     /translation="MKRLAVLMAVCFVDMLGLMLVAPLMPFYALRLHAPEWLVGPLIA
                     AFAVAQLVSSPVWGKFSDRYGRRPALMIGLTASAFAYVIFGFANSLWLLFASRIVQGL
                     GGGTTGVAQAYVADTMEPSERAKALGWLSAATSAGVVIGPFIGSAATNFGTAAPGIVA
                     ALLVLVNVAFAWKWLPESRLGHPRTASGHFAGSPGVRTVKQALLEIVYHPNRPAHRVI
                     WIYVVGMLALNIVIGVLALYLRDTFGVTEKTIGYFFPIFGAVGVLMRASLVGWINKRI
                     GEVRTMQLGILLLMVGLVLMPLPAAVLPPSAALPLF"
BASE COUNT          580 a         1523 c         1773 g          810 t
ORIGIN      
        1 gggagcggtt cacgtcgagc ggcaacgtcg cgcaggtgca cgccttctgc gaccgctccc
       61 cgctcctcgc tcccggttct ccttggcgct cctcgcgctc gccgtcatcg gatgctcagg
      121 ggcgggcaag actcccctgg tcatctacac cccccacggc cgcgacctgt tgacgctgct
      181 gaaggcgcgc tacgagcgat tgcatccgga ggtggacctc cggtggctcg acatggggtc
      241 gcaggaagtg tatgaccgcg tccgcagcga gcgcgccaat ccgcagggag acgtctggtt
      301 cggtggaccg gcctcgatct tcgccctcgg cgccggcgac tcgctgctcg agccctacca
      361 cccggcctgg gcggaccaca tcagtcccgg gggacgaggg ccggccgatc tgtacttctc
      421 cgcctacgag acgcccgccg tgatcgtcta tgccgagcag gcgctgacgc cggcgcaggc
      481 gccgcaggat tgggacgatc tgctcgatgc gaagtggaag ggcaagatcc tgattcgtga
      541 cccgatcgcc tcggggacga tgcgggccgt gtgggggatg atcatcgagc gcggcctgaa
      601 gcagaccggt gatacggcgg ccggcttcga ctggctgcgg cgcctcgacg cgcaaacaaa
      661 ggaatatgtg ctcaacgggc cgctgctgga ccagaagatc gtccgccagg aggggctggt
      721 gacgatctgg gacctccccg acatccagct caacaagcgc gacggcctgc cgctgggcta
      781 cgtgttcccg acgagcggta cgcccgcgat cgaggacgcg attgggatca ttcgcaacgc
      841 gccgcaccgt gacgcggcgc gcgccttcgt cgagtgggtg gggagcaccg aggcgcagct
      901 ggtcgccgcg cgtgaggcgt accggctgcc ggcgcgggag gacttgccgg tcgactcgct
      961 ccccgaatgg gtgcgggacg tgcgccgccg tctcgtcacc gccgacatgg actgggacct
     1021 gctggcccgc cagggcggcg cgtggatgcg gtattgggac gagaatgtcg gggggaaggg
     1081 ggaaggggga agggggaagg gtgggcgttg aggaggcgtt tctctcgctc gacgagcttc
     1141 gcgtccgctt cagcgacacc gtcacgatag ggccggtttc actttcgatc gcgcgggggg
     1201 agatcctcgc gctgctgggg ccgtcgggga gcgggaagac caccctgttg cgcatgctgg
     1261 cggggttcga gcggcccgac ggtggcacgg tacggatcga gggcgaggac gtgacggcgg
     1321 tgccgcctgc gcgccgccgg gtggggatgg tgttccagca ttacgcgttg ttcccgcatc
     1381 tcgatgtggg ggagaacgtc gcgttcggac tgcacgggac gagggacgag ggacggggga
     1441 cgagtgctgt ggtagcggag gccctcgcgc tggtcgacct cgccgggttc gagcggcgca
     1501 aagtgcagga gctgtcgggc gggcagcagc agcgggtggc gctggcacgg gcgctggctc
     1561 ccgcgccccg ggtcctggcg ctggacgagc cgctctccaa tctcgatccc agcctgcgcg
     1621 agcgcacgcg ccgcgagctg cgccagctca tcaagcgcat cggcatcacg gccgtattcg
     1681 tcacccacga gcaggacgag gcgttcgatc tcggggatcg gatcgcggtg ctgagcgccg
     1741 ggcggctgaa ccaggtcggc acaccggagg atctgtacga gcggccggcc aacctgttcg
     1801 tggggacgtt cgtgggacgc gccaatgtgc tgcgcggtgc gacggcgcgc gccctcggcg
     1861 ccgccgacgg cgaggcgatc ctggtgcgtc cggagcagct gcgcttcggc gccggggagg
     1921 gagaacgtgg cgggctgaac ggcgtcgtgg ccgagcgccg ctacaccggc gccgccgcgt
     1981 tctttcaggt cgatctcgag acgggtgagc gcgtggaggt cgaggccgag ccgcaggcag
     2041 cgcgcgtggg cgatcgtgtc cgcatcgggg cggtacgcac actgcgcttc gcgggtccgc
     2101 agggggaagg gtgacgcgca gccggcgggc gggcggtgga cgcagcgggc cggccggcgt
     2161 agcgctcacg ctgctgctgg cctggctcgt gctctatccg atcgtgctgc tggtggtgga
     2221 tggtctgcat ggcgacgcgc tgcgcacgtt cgtcgagcgg agcggggagt ggcgcgcgtt
     2281 gtgggcgagc gtgtggatct cgctggccag cgtcgtcctg gccgcggtga tcggcgtgcc
     2341 gctggcgttt ctgttcgagt ggttcgagtt cccggggcgc aaattgctgg ggtcgctgat
     2401 cgcgctcccc gccgtgctgc cgccgttcgt cggcgtcatc gcgttcctgt tcctgtacgg
     2461 tgagtcgggt ttcgccgcgc gcttcctcca gttcgtgttc cggtttcagc aagcgccgtg
     2521 gcacctgcag ggggccgggg cgattctcct ggtgcatgcg tactcgatgt acgtctactt
     2581 ctacctgttc gcgcgtgccg gcctggcccg gctcgacgcc gccacgctgg aggcggcgca
     2641 ggcgctcggc gccgggcggt ggcggaccat cacgcgggtc gtgctgccgc tgatccgtcc
     2701 gtcgctcgcg ggcgcggcgc tgctgacctt catgacgtcg ctcggctcgt tcagcgcacc
     2761 gtacgtgttc ggcggcgggt tccgggtgat gacgacccag atcttcgcct cgaaacagaa
     2821 cggcgagctc ggggtggcga tggtggagac ggtggcgctg gcgctggtcg ccctggcggg
     2881 tctcgccctg ctgcggcgca ccgaggggga cgccatcctc gccgggctcg ggaaggggac
     2941 ggccccgcgg cgacgcgccc tgcgccgccc gctggtacgg gccgccacga cgctcgtggg
     3001 ctggggactc gcggtgctgc tgctgctgcc gcacgcgacc ctggtgctcg tctccctcgt
     3061 tccctacgca acctggacga ccgagacgct gccgccggtc ttaagcttgg tgaactatgg
     3121 gcgcctgttc gccgagcccg agcggctcga gccgctggtg aactcgttct ggatggccgc
     3181 ggtgagcacg agcctggccg tcgcggtcgg actggcggcg gggtggctcg tggtgcgtcg
     3241 tcgggtcgcg ctgcgccgct ggatcgaagg gctgctcgcg ctgccgtggg cgctgcctgg
     3301 cacggtgttc gccatcgccc tggcgaccgc gttcagcgtg tcggcgccgc tgcagctgcg
     3361 ctggatcctg gtcggcacgg tggcgatcct gccgctcgcg tatttcgtgc ggaatctccc
     3421 ggtcaccgga cgggcgatcc tcgccgggta ccggcagctc gacccggcgc tcgaggaagc
     3481 ggcggcgagt ctcggggcgg gtccctggcg cacgctacgc cgcgtgacgc tgccgcagct
     3541 caggccggcg ctcgccgcgg gggcgagtct cgcgttcgtg ggagcgctgg gggatttcgt
     3601 gacgtcgatc ctgctctata cctatcgcaa ccggcccatc tcgatggaga tcaacgcggc
     3661 gctgcgggtg aacgaaatcg gtgtggcggc ggcgttcggc gtcgtgctga tggtggttag
     3721 cgcggtcgtg ctgtcgatcg gagcgcgccg gtgaagcgac tcgcggtgct gatggcggtc
     3781 tgcttcgtgg acatgctggg gctgatgctg gtagcaccgc tgatgccgtt ctacgccctg
     3841 cggttgcacg cgccggagtg gctggtcggc ccgttaatcg cggcattcgc ggtggcccag
     3901 ctcgtgtcgt ctccggtgtg ggggaagttt tcggaccggt acggccggcg gcccgcgctg
     3961 atgatcgggc tcacggcctc ggccttcgcc tacgtcattt tcggattcgc caacagcctg
     4021 tggctgctgt tcgcgtcgcg catcgtgcag ggattgggcg gtggcacgac cggcgtcgcc
     4081 caggcctacg tcgccgacac gatggagccg agcgagcggg ccaaggccct cggctggctc
     4141 tccgccgcga cgagcgccgg cgtggtcatc ggcccgttca tcgggtcggc ggccaccaat
     4201 ttcggaacgg cggcgcccgg gatcgtcgcc gcactgctgg tgctcgtgaa cgtcgcattc
     4261 gcctggaagt ggctgcccga atcccgcctc gggcacccgc gcaccgccag cgggcacttc
     4321 gccggatcgc ccggcgtccg cacggtcaag caggcgctgc tggagatcgt ctaccacccg
     4381 aaccggccgg cccatcgggt gatctggatc tacgtggtgg gcatgctcgc gttgaacatc
     4441 gtcatcggcg tgctggcgct ctacctgagg gacaccttcg gcgtcaccga gaagacgatc
     4501 ggctacttct tcccgatctt cggcgcggtg ggcgtgctga tgcgcgcctc gctcgtgggc
     4561 tggatcaaca agcggatcgg tgaagtccgc accatgcagc tcggcatcct cctgctcatg
     4621 gtcggcctcg tcctcatgcc gctgccggcg gccgtgctgc cgccgtcggc cgcattgccc
     4681 ctgttc
//
LOCUS       DBNGZW010000406         2147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1538320, whole genome shotgun sequence.
ACCESSION   DBNGZW010000406 DBNGZW010000000
VERSION     DBNGZW010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1538320"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="AC16JC_09595"
     CDS             complement(<1..483)
                     /locus_tag="AC16JC_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164986.1"
                     /translation="MRDRVGSLFLSLLGMGAAVASGQSTLPLKHAPKATTPAITAADL
                     MTRLYIYADDSMQGREAGTPGNVKATNYIAAELRRLGLQPAGDSGTFFQTIPLKTRSF
                     DTTSTFTVAGTPLVAFQDYLALSRQPASHAGVATVFGGTLGDSMRTISAEQAAGKLVV
                     L"
     gene            628..>2147
                     /locus_tag="AC16JC_09600"
     CDS             628..>2147
                     /locus_tag="AC16JC_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HIR5164987.1"
                     /translation="MMPRCALFVAGLVAATPVRAQQPPALTADDYARAERFLGYNTNP
                     LVSGAAVRPGWLAADRLWYLNTTAEGVEYVLVDPASRTRAPAFDHARIATALSRAADS
                     TYDARHLTLGALSFSARLDSISFDARGRRWSCDVRGAGCGAAAPRDSGPRNAVLSPDG
                     KRAAFIRDDNLWMRDLGTGRETQLTTDGVPDFGYATDNAGWVRSDRPVLLWSPNSKKI
                     ATFQQDQRGVGEMYLVRTQVGHPVLERWKYPLPGDSVVAMIQRVIIDVDAPRVIRLQL
                     PPDQHRSTLCDHVVCRGEWADVEWTPDASHLVFVSTSRDHRHEWVRVADAATGAVRDV
                     FDESVTTFFESGAGRVNWHVLPATNQILWFSERSDWGHLYRYDLGTGKLLGQVTSGSW
                     NVLQVLRVDEASRTLLFSGAGREPGDPYFRRLYSVRLDGSRLTLLTPEDADHEVTLAP
                     DGRYFVDSYSRPDVPPVTVLRDPTGRIVLTLERADISRLTATGWRPPLPITVKARDG"
BASE COUNT          329 a          759 c          732 g          327 t
ORIGIN      
        1 caggacgacg agcttgcccg ccgcctgctc cgcggaaatc gtccgcatcg agtcgcccag
       61 ggtcccgccg aataccgtcg cgaccccggc gtgactggcg ggctggcggc tgagcgccag
      121 gtagtcctgg aacgcgacga gcggcgtgcc ggccaccgtg aaggtggagg tcgtatcgaa
      181 gctgcgcgtc ttgagaggaa tggtctggaa gaaggtgccg ctgtcgccgg cgggctggag
      241 ccccagccgc cgcagctcgg cggcgatgta gttggtcgcc ttcacgttgc cgggagtgcc
      301 cgcttcgcgg ccctgcatcg aatcgtcggc gtagatgtag agccgggtca tcaggtccgc
      361 ggccgtgatc gccggagtgg tggctttggg ggcgtgcttc aacggaaggg tgctctgacc
      421 gctcgccacg gcggccccca tcccgagcag agagagaaac agagaaccga cacggtcgcg
      481 catcaaacgc ctccaagaag agaacgacgg cgtgcgagaa cagggtgata cctgaagcta
      541 tacgatggcc gaccccgatg tgttggggga agccccctct ccttcggcgt gacatattgg
      601 gaccgaacgc acaccttccc ggagtccatg atgccccgct gtgccctgtt cgtcgccggc
      661 ctcgtcgccg ccacccccgt ccgagcccag cagcctcctg cgctgacggc cgacgactac
      721 gcccgcgccg agcggttcct gggctacaac accaacccac tggtctcggg tgccgccgtg
      781 cgacccggat ggctcgccgc cgaccgcctc tggtatctca acaccaccgc cgagggcgtc
      841 gagtacgtgc tggtcgatcc cgccagccgg acgcgcgcgc ccgccttcga tcacgcccgg
      901 atcgcgacgg cgctgtcccg cgccgccgac tcgacctacg atgcccgtca cctgacgctc
      961 ggcgccctga gcttctccgc gcgactcgac tcgatctcct tcgatgcgcg gggccggcgc
     1021 tggagctgcg acgtgcgtgg cgctggctgc ggcgcggccg cgccccgcga ctccgggccg
     1081 cgcaacgccg tgctctcgcc cgacgggaag cgggcggcgt tcatccgcga cgacaacctg
     1141 tggatgcgcg atctgggcac gggacgggag acgcagctca ccacggacgg tgtaccggac
     1201 ttcggctacg ccaccgacaa cgccggctgg gtgcggagcg accggccggt actgctctgg
     1261 tctcccaact cgaagaagat cgccacgttc cagcaggacc agcgcggcgt gggcgagatg
     1321 tacctcgtgc gcacgcaggt cggccacccg gtgctggaac gctggaagta cccgctcccc
     1381 ggagacagtg tggtcgcgat gatccagcgc gtgatcatcg acgtggacgc gccccgcgtg
     1441 atccggctgc agctgccgcc ggaccagcat cgctcgaccc tgtgcgatca tgtggtgtgc
     1501 cgcggagaat gggccgacgt cgagtggacg ccggacgcgt cgcatctcgt cttcgtctcg
     1561 acttcacgcg atcaccgcca cgagtgggtg cgggtcgccg atgccgcgac gggcgcggtg
     1621 cgcgatgtgt tcgacgagtc ggtcacgacg ttcttcgagt ccggcgcggg ccgcgtgaac
     1681 tggcacgtgc tcccggccac gaaccagatc ctgtggttct ccgagcgcag cgactggggc
     1741 catctgtacc gctacgatct cggaaccggg aagctgctcg ggcaggtcac gagcggcagc
     1801 tggaacgtcc tgcaggtgct gcgcgtggac gaagcgagtc gcaccctctt attcagcggc
     1861 gcgggacgcg agccgggcga cccgtacttc cgccgtctct acagcgtccg gttggacggc
     1921 agccggttga ccctgctcac accggaggat gcggaccacg aggtcacgct ggcgcccgac
     1981 ggccgttact tcgtggacag ctactcgcgc ccggacgtcc ccccggtcac cgtgctgcgc
     2041 gacccgaccg gccgcatcgt cctcacgctc gagcgcgccg acatctcacg cctcacggcc
     2101 acagggtggc ggccaccgct gcccatcacg gtgaaggcgc gcgacgg
//
LOCUS       DBNGZW010000407         1947 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1731507, whole genome shotgun sequence.
ACCESSION   DBNGZW010000407 DBNGZW010000000
VERSION     DBNGZW010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1947
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1731507"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="AC16JC_09605"
     CDS             complement(<1..534)
                     /locus_tag="AC16JC_09605"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HIR5164988.1"
                     /translation="MSRLPVVLAITGASGAPYGVRLLEVLATHHVPTWLLVSGHGWRL
                     LEEECGIEGEPALRKATGGDWHSVTVFDDKDRGARPASGSARSAGMVVCPCSMGTVSA
                     IAQGSSRSLIERAADVTLKERRRLILVPRESPLSLVHLRNLVSVTEAGAVVLPAAPGF
                     YHRPRKVEELVDFVVQRV"
     gene            complement(677..1570)
                     /locus_tag="AC16JC_09610"
     CDS             complement(677..1570)
                     /locus_tag="AC16JC_09610"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     polyprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HIR5164989.1"
                     /translation="MTVREGQTLEGQSLLVRYANFVRLPHTVFALPFALLGVVVASRE
                     APVTWRTIGLVALAFTAARFVAMGFNRIADRELDARNPRTRGRELPAGRLTLAQAWGA
                     VVVAALLFEGAAWALNPLCFALSPIALLVITLYSYAKRFTWWSHLWLGLGDAIAGPAG
                     YLAVAGAWSRPGWLLWALAAAVTFWVGGFDIFYALQDEQFDRAEGLHSAVVRFGQRHG
                     ILLAKLLHALALVALVLFGAGAGLGWVYYAGVAIGASVIAYEHRLVRPGDLSRLNAAF
                     FTMNGIVSIVVFLGALGDRLL"
     gene            complement(1567..>1947)
                     /locus_tag="AC16JC_09615"
     CDS             complement(1567..>1947)
                     /locus_tag="AC16JC_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HIR5164990.1"
                     /translation="VFVSIDKQYPGQAYKVMNGLWGQGLMSLAKVIVVVDRDVNVRDP
                     KEAWWVALNNIDPERDVRFTLGPIDVLDHSSRAFTFGSKMGIDATRKWKEEGFDRPWP
                     DRIVMDDATRGRVDAMWRELGIEP"
BASE COUNT          336 a          701 c          635 g          275 t
ORIGIN      
        1 aacacgctgc acgacgaaat ccaccagttc ctccaccttc ctcggacgat ggtagaaccc
       61 gggcgcggcg ggcagcacga ccgcccccgc ctcggtgacc gacacgagat tccggaggtg
      121 cacgagcgag agcggtgatt cccgcgggac gaggatcagg cgtctccgct ccttcagcgt
      181 cacgtcggcc gcccgctcga tgaggctgcg ggagctgccc tgggcgatcg ccgagaccgt
      241 gcccatcgag cagggacaga cgaccatccc cgcagagcgc gccgagcccg acgccggccg
      301 ggcgccgcgg tccttgtcgt cgaacaccgt cacgctgtgc cagtccccgc ccgtcgcctt
      361 gcgcagcgcg ggctcacctt cgatgccgca ttcctcctcg agcaaccgcc acccgtgccc
      421 ggacacaaga agccaggtcg gcacgtggtg cgtcgccagc acctccagca atcggacgcc
      481 gtagggtgcg cccgaggcgc cagtaatcgc gagcacaacg gggagtctgc tcatcgcccg
      541 cggcggttgg gggtgacgga ccttctgggg tgcgccggcg gtctcggcgg gaagggggaa
      601 gagggtgggg ggaaggggac tttcttcgac ccgtgtggtt tacccttccc tcttccctct
      661 tccctcttcc ctctgttcat aacagccgat cccccaacgc gccgaggaac acgacgatac
      721 tcacgatccc gttcatcgtg aaaaaggccg cgttgagacg cgacaaatcg ccgggacgga
      781 ccagccggtg ttcgtaggcg atgaccgatg cgccgatcgc cacgccggcg taatagaccc
      841 agcccaatcc cgcgcccgcg ccgaacagca ccagcgcgac gagggcgagc gcgtggagca
      901 gcttggcgag cagaatgccg tgtcgctgtc cgaaccggac gaccgccgag tggagtccct
      961 cggcacggtc gaactgctcg tcctgcaggg cgtagaagat gtcgaatccg ccgacccaga
     1021 acgtcaccgc cgccgcgagc gcccagagca gccagcccgg ccggctccac gcgcccgcca
     1081 ctgccaggta gccggccggt cccgcgatcg cgtctcccag gccgagccag aggtgcgacc
     1141 accaggtgaa gcgcttggcg tagctgtaaa gagtgatcac cagcagggcg atgggggaga
     1201 gcgcgaagca cagcgggttg agggcccacg ccgccccttc gaacagcagc gcggcgacga
     1261 ccacggcgcc ccacgcctgt gccagcgtga gccgcccggc gggcaactcg cggccgcgcg
     1321 tgcgcggatt gcgcgcgtcg agctcgcggt ccgcgatccg gttgaacccc atcgccacga
     1381 aacgcgccgc ggtgaacgcg agcgcgacga gtccgatcgt gcgccaggtg accggcgcct
     1441 cgcgcgaggc gaccaccacg cccagcagcg cgaacggcag ggcgaacacg gtgtggggca
     1501 gccggacgaa attcgcgtac cggacgagca ggctctggcc ttcgagagtc tggccttcgc
     1561 gcaccgtcac ggctcgattc ccagctcgcg ccacatggcg tcgaccctcc cccgggtcgc
     1621 gtcgtccatc acgatccggt cgggccaggg gcggtcgaag ccctcttcct tccatttgcg
     1681 cgtggcgtcg attcccatct tggagccgaa cgtgaacgcg cggctcgaat gatcgagcac
     1741 gtcgatcggc cccagcgtga agcgcacatc gcgctcggga tcgatgttat tgagcgccac
     1801 ccaccacgct tccttcggat cgcgcacgtt cacgtcccgg tccaccacga cgatgacctt
     1861 ggcgagggac atcaggccct gcccccacag gccgttcatc accttgtagg cctggccggg
     1921 atactgcttg tcgatgctca cgaagac
//
LOCUS       DBNGZW010000408         2514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_96489, whole genome shotgun sequence.
ACCESSION   DBNGZW010000408 DBNGZW010000000
VERSION     DBNGZW010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_96489"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="AC16JC_09620"
     CDS             complement(<1..368)
                     /locus_tag="AC16JC_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164991.1"
                     /translation="MPLRRWRNGFLAIVLPSTLLAQQPKSAARSAPQLFLFDVDTLQA
                     NRVWCGLNNLGELCVNPDGSPVIGGGFWPRGTVDQYVFNSGLQVAGIIPGNAGGGRAS
                     FPWAGDTVGAYFHDTRGDQVA"
     gene            406..897
                     /locus_tag="AC16JC_09625"
     CDS             406..897
                     /locus_tag="AC16JC_09625"
                     /inference="COORDINATES: protein motif:HMM:NF013440.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HIR5164992.1"
                     /translation="MLDELKARLQAEVERLNHEISFVLPKEIEKARAHGDLRENSEYK
                     AALERQQFAQARVHHLRLRLSKLSDVSDKDIPKDRVGFGSRVTVQDVETKAKETYALT
                     LGEFIEGDDNAQEMPVSMASPLGKALLGGKVGETVSITLPMGKRKLKIMELVTVHEIA
                     RGA"
     gene            complement(900..1460)
                     /locus_tag="AC16JC_09630"
     CDS             complement(900..1460)
                     /locus_tag="AC16JC_09630"
                     /inference="COORDINATES: protein motif:HMM:NF021604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor domain-containing protein"
                     /protein_id="HIR5164993.1"
                     /translation="MSDWLGMAAPPRRPRPELKARVLARAYASSRRSWAWPLAAAALL
                     LLGLAGGGVLWRRVAQLQSDLAATRDTLGLLRQPGTRVVAIPVVTQGRPGELTIFADS
                     VSHRWLVTCHNLTPNAPGEAYQLWFVTEQGMRTAALMPMDQSNPMVLAFDMPQGAGKV
                     MGMAMSVEPRAGSIEPKGPMVFHVDL"
     gene            complement(1457..2056)
                     /locus_tag="AC16JC_09635"
     CDS             complement(1457..2056)
                     /locus_tag="AC16JC_09635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5164994.1"
                     /translation="MSRSERLTDEEQRSLLARLRAREEAALAELYDRLSPWVLGAAHR
                     ILGDADEADEVLSDVFVQVWRRIDQHDASRGPLTPWVLSIARNRSLDVLRRRGRWRRK
                     VALVEAEAKVEGDEPAVPADLAEASVPGWPVHQAVHQALAALPEEQRRVVRLAYFEGL
                     SHSEIAQRTGDPLGTVKTRLRLAQQKLEESLRHLKDWTA"
     gene            complement(2053..>2514)
                     /locus_tag="AC16JC_09640"
     CDS             complement(2053..>2514)
                     /locus_tag="AC16JC_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5164995.1"
                     /translation="PIHYAVADWSTRAVQAVFFAAALAVALVLAYRFRRHPRDWADPR
                     TAAELAILLGAMVVVDPLAWKAHYVVLIVPNTFAWWALRRFPADAPGRTWRWALWWGS
                     FACITLSAPTLVGSHWRDVLESLNVILIGALLLLVLAVSLVDAPRRAGEST"
BASE COUNT          443 a          893 c          818 g          360 t
ORIGIN      
        1 gccacctggt cgccccgggt gtcgtggaag tacgcgccca ccgtgtcccc tgcccacggg
       61 aacgaggccc ggcctcctcc tgcgttgccc ggaatgatcc ccgcgacctg gagtccggag
      121 ttgaagacgt attgatcgac cgtcccacgc ggccagaacc ctccgccgat gaccggcgag
      181 ccatccgggt tgacgcacaa ctcaccgagg ttgttcaggc cgcaccagac gcgattcgcc
      241 tgcaacgtgt cgacgtcgaa caggaacaac tgcggggcgg atcgagccgc cgacttcggc
      301 tgctgggcga ggagcgtgga cggcaggacg atggcgagaa aaccgttccg ccatctccgg
      361 agtggcatca tgcgcagata atgtctagtt tctttccctc acgccatgct cgatgaactg
      421 aaggcccggc tccaggccga ggtcgaacgc ttgaaccacg agatctcctt cgtgctgccg
      481 aaagagatcg agaaggcgcg cgcccacggc gatcttcgcg agaactccga gtacaaggcc
      541 gcactggagc gccagcagtt cgcccaggcg cgcgtgcacc atctgcgcct gcgcctgtcc
      601 aaactgtcgg acgtgtcgga caaggacatc ccgaaggacc gcgtgggatt cggctcgcgc
      661 gtgaccgtgc aagacgtgga gacgaaagcg aaggagacct acgcgctcac gctcggcgag
      721 ttcatcgagg gtgacgacaa tgcccaggag atgccggtgt cgatggcctc acccctcggc
      781 aaggcgctgc tcggtggcaa ggtcggagaa accgtctcga tcaccctgcc gatggggaag
      841 cgcaaactga agatcatgga gctcgtgacg gtgcacgaga tagcccgggg agcgtagcgc
      901 tacagatcca cgtgaaacac catcgggccc ttcggctcga tggatcccgc ccgcggttcg
      961 acgctcatcg ccatgcccat caccttgccg gcgccctgcg gcatgtcgaa cgccagcacc
     1021 atcggattgg attgatccat gggcatcagc gccgccgtgc gcatcccctg ctccgtgacg
     1081 aaccacagct ggtacgcctc gcccggcgcg ttgggcgtca ggttgtgaca ggtcacgagc
     1141 cagcggtgcg acacactgtc ggcaaagatc gtaagctcgc cggggcgccc ctgcgtcacg
     1201 accgggatgg caacgacgcg tgtccccggt tgccgcagca ggccgagggt gtcgcgtgtc
     1261 gcggcgaggt cggattggag ctgcgcgacg cgacgccaca gcacgcctcc tcccgcgagt
     1321 ccgagcagga ggagcgccgc ggccgcgagc ggccaggccc acgagcgccg cgaagaggcg
     1381 tacgcccggg cgagcacccg cgccttcagc tccggccgcg ggcgtcgcgg tggcgcggcc
     1441 attcccagcc agtcgctcat gccgtccagt ccttcaggtg tcgcaagctc tcctccagct
     1501 tctgttgtgc gagtcgcaac cgcgtcttca ccgtccccag cggatcgccg gtgcgctggg
     1561 cgatctccga atgcgacagc ccctcgaagt aggccagccg caccacccgt cgctgctcct
     1621 cggggagcgc tgcgagggcc tgatgcacgg cctgatgcac cggccatccg ggcaccgacg
     1681 cctcggcgag gtcggcggga accgccggct cgtcgccttc caccttcgcc tccgcttcga
     1741 ccagcgccac ctttcgccgc caccgccccc gccggcgcag cacgtcgagc gagcggttgc
     1801 gggcgatcga gagcacccac ggcgtcaacg ggccgcgcga tgcgtcgtgc tggtcgatcc
     1861 gccgccacac ctgcacgaac acgtcgctca gcacctcgtc ggcctcgtcg gcatcaccga
     1921 ggatgcgatg cgccgcgccc agcacccagg gggacaggcg atcgtatagc tccgccagcg
     1981 ccgcttcctc gcgcgcgcgc aggcgggcca gaaggctgcg ttgttcttca tcggtaagac
     2041 gctctgaccg gctcacgtag attccccggc tcgcctcggg gcgtcgacca acgacaccgc
     2101 cagcaccagg agcagcaggg ccccgatcag gattacgttg agcgattcca gcacgtcgcg
     2161 ccagtgcgag ccgaccagcg tcggagcgct gagcgtgatg caggcgaagc tcccccacca
     2221 tagcgcccag cgccacgtcc ggcccggagc gtccgccggg aaacggcgca gcgcccacca
     2281 ggcgaacgta ttcggcacga tgagcacgac gtaatgcgcc ttccacgcca gtggatccac
     2341 caccaccatc gcgcccagca ggattgcgag ttccgcggcc gtacgtggat cggcccaatc
     2401 gcgtgggtgg cgccgaaacc ggtaggcgag aacgagcgcc accgccagcg cggccgcgaa
     2461 gaacaccgcc tggacggcgc gggtggacca gtcggccacc gcatagtgaa tcgg
//
LOCUS       DBNGZW010000409         7284 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_96497, whole genome shotgun sequence.
ACCESSION   DBNGZW010000409 DBNGZW010000000
VERSION     DBNGZW010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_96497"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="AC16JC_09645"
     CDS             <1..341
                     /locus_tag="AC16JC_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor-like protein"
                     /protein_id="HIR5164996.1"
                     /translation="VRVTHIPSGLVVQCQDQKSQLQNKIKALEILRSRLLDRMIAEQE
                     AERARARRAQVGTGDRSAKVRTYNFPQNRVTDHRIGYTAHNISDVLDGRLDDLIDALR
                     LAGERERLSA"
     gene            334..1212
                     /gene="prmC"
                     /locus_tag="AC16JC_09650"
     CDS             334..1212
                     /gene="prmC"
                     /locus_tag="AC16JC_09650"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR5164997.1"
                     /translation="MPDLAASPATIGAELEAAAALLGDAGVAEPRREANRLWAAIAGG
                     RPGDAWLRRDRPSDPRTAARFREAVRDRAAGVPFAYAAGTAAFRALELAIDRRALIPR
                     PETEGLVDLVLRWCRENGEWGVGRWGIAADIGTGSGCIALSLAVEGAFERVVATDQSE
                     AAVSLARENVARVAPSVPVEVRLGDLLAPLGDAHHRVIVANPPYLTEDEWTALEPGVR
                     DCEPKSALASGADGLEVTRRLLAGARAVLEPAGLLALEIDERRAAAIRQLAEATGWRV
                     SIHRDVFDRPRYALAV"
     gene            1215..1580
                     /locus_tag="AC16JC_09655"
     CDS             1215..1580
                     /locus_tag="AC16JC_09655"
                     /inference="COORDINATES: protein motif:HMM:NF017902.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA];
                     GO_process: GO:0043934 - sporulation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HIR5164998.1"
                     /translation="MEDSVIDEKAQDLGRLIGQTEEYKALKRASERLREDADCQRLLA
                     DMEKLAGTIETAARDGKEPTPEQAEQYDKAVQSIQVNQVYQQMAVAQANFEKLMAKVN
                     ARIYEGIQKGAASPIITLG"
     gene            1586..2218
                     /gene="tmk"
                     /locus_tag="AC16JC_09660"
     CDS             1586..2218
                     /gene="tmk"
                     /locus_tag="AC16JC_09660"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013971580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR5164999.1"
                     /translation="MAGTFVVVEGPEGAGKSTLVRALGARLLAEGMQVLLVREPGGTP
                     VAEAARKVALRSRHELSAAAELFLILAARADLVAQVIRPALDAGQVVLADRFDLSTRA
                     YQVAGRGLPAHDVDAALRLATGGLVPDLTLVLDIPVDIGRERQRKARKVQDRFEREDD
                     AFHERVLATYRNAAGPGVVHLDATPSKRTVLDAAWRELQAVTVLSTRGIS"
     gene            2215..3828
                     /locus_tag="AC16JC_09665"
     CDS             2215..3828
                     /locus_tag="AC16JC_09665"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR5165000.1"
                     /translation="MSRKWLLVSVMTGVALVTGGWLLQRDSSPQGTVYQQARLFDDVL
                     SRVADFYVDSIDERKLYEMAIDGMLDQLHDPYSTYLKPSDFRSLNEQTTGDYGGLGLQ
                     IEVRNGWITVVAPLPDTPAERAGIQTGDEIVTLDGRSTEGWKQDEAVNQLRGTAGSTV
                     ELKIRRIGVEEPITFKLTRAKIHIRSVQVSIMLDDRVGYVSLNPVSQTSADEVAQAVD
                     SLIAKGMKSLVFDLRGNPGGLLTQGIAVSDLFLDPGQEIVATRGRAPDASHTYRDSRP
                     QRWPKLPIVTLVNAGTASAAEIIAGALQDHDRALVVGTPTFGKGLVQSLYMLSPQTAL
                     KLTTARWYTPSGRTIQRASRSEAEQLAALEAAAHGTDTLSDSAPVFHTDAGRSVRGGG
                     GIRPDMFERPDTFTTAERAFVRGLGSNVATFRDVLTGYALELKSGHALASAQFAVTPA
                     MVDEVIRRMRQRGAAVSDTLAEGARSLIRDELGYEATRYVFGRGAEVLRRMHDDHQVQ
                     QALDFARRARAPQDLFALVTPGGAQQRSP"
     gene            3825..4727
                     /locus_tag="AC16JC_09670"
     CDS             3825..4727
                     /locus_tag="AC16JC_09670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HIR5165001.1"
                     /translation="MTPGPGPAPLVVGVVGPGRAGTGLALAWAQAGHDVRLHGRHPKP
                     VPPPLTLTVGAANEPPPWLAAVSVVVLAVPDDAIAPLARSLARAGSLAAHQVVLHLSG
                     LLDHEALAALAASGAALGSLHPLQTLVEAEQVPMHLRGAWAAVEGTPPAVATAERLAR
                     DIGLRPFRVRPDDKARYHAGAVFASNYFVVVEAVAQELLRSTGLSETDAWSALAPLVA
                     GTLDNLARRGPVAALTGPVARGDAETVTRHLAVLSGDQRELYRRLGTLALDLARRRGL
                     EPGAAARVARALATDPPPGRPGER"
     gene            complement(4690..5373)
                     /locus_tag="AC16JC_09675"
     CDS             complement(4690..5373)
                     /locus_tag="AC16JC_09675"
                     /inference="COORDINATES: protein motif:HMM:NF015510.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LolA family protein"
                     /protein_id="HIR5165002.1"
                     /translation="MDGLANGRMALGVALLVALPAPSVYPSIRLSDQDPWPILDHASA
                     AYQGVQTLAANFVQIITNPMVGEPDTTRGRLFQQRPSRFAMRFTSPAGDRIVADGRYL
                     WLYTPSTEPGQVIRSRIPRTGTGPNLIGQFVERPRERFTARYVRADSSAAGWADVIAL
                     TPRNDSQPYSSAVIWVGRGDGLVRRVDLVETSGQERSVRLSNLQANAAVPSREFRFSA
                     PRGVRVVDQ"
     gene            complement(5378..6418)
                     /gene="aroF"
                     /locus_tag="AC16JC_09680"
     CDS             complement(5378..6418)
                     /gene="aroF"
                     /locus_tag="AC16JC_09680"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020720256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR5165003.1"
                     /translation="MLIVMRHGATEEEIQRVVKVIRDMGYQARPMPGAQRTAVGLVGN
                     DGRVESSRLEGLPGVQDVIHVSKPYKQVSREWQPENTVVRLPGGIAVGGNDIVVIAGP
                     CSVESEEQIVQAARLVKDAGAVILRGGAFKPRTSPYSFQGLGRPGLRLLAKARAETGM
                     LICTEAMDPDGVGWVSEIADIVQIGARNMQNFSLLRAAGRAGKPGLLKRGISATITEL
                     LLSAEYVLAEGNHDVILCERGVRGFDTATRNIFDLSAIPVVQKLSHLPIIADPSHATG
                     LREKVIPMARAAVAAGADGVMVEVHPDPEAAKSDGAQSLFPDQFAQLMREVRVIAEVI
                     GRRIAEPAAVGA"
     gene            complement(6412..7191)
                     /locus_tag="AC16JC_09685"
     CDS             complement(6412..7191)
                     /locus_tag="AC16JC_09685"
                     /inference="COORDINATES: protein motif:HMM:NF049367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5165004.1"
                     /translation="MELITVGTGTVAPSARRTAAAHYVERGEVRALLDCGPGTLHRLA
                     QFGLPWSDITHVALSHFHPDHFADLPMLVYALKYTTVPPRSEPLVVLGPRGVVRLVKA
                     LAEGYGSWLLGPGFPLLIMDVEPGEPFPLDADLSLESFPVPHTEESVAFSLTAPEGRL
                     VYTGDTGPSPELARWATGCTVLLAECSLPEAMALDIHLTPERAGELARAAGAKRLVLT
                     HFYPSVENSDPAQRAASRFGGPVTAAVDGERFTIAQEKTSC"
BASE COUNT         1109 a         2498 c         2623 g         1054 t
ORIGIN      
        1 cggtccgcgt gacccacatc cccagcggcc tcgtcgtcca gtgccaggat cagaaatccc
       61 agctgcagaa caagatcaag gcgctggaga tcctgcgctc gcggctgctc gaccggatga
      121 tcgccgaaca ggaagccgag cgcgcgcgcg cgcggcgcgc acaggtgggg accggggacc
      181 gctccgccaa ggtgcgcaca tacaactttc cccagaatcg cgtcaccgat caccgtatcg
      241 gctacaccgc tcacaacatc tccgacgtgc tcgacgggcg gctcgacgac ctcatcgatg
      301 cgctgcggct ggcgggtgag cgggaacggc tcagtgcctg atctggccgc gagtccggcg
      361 accatcggcg ctgagctgga ggccgccgcc gcgctcctgg gcgacgccgg cgtggccgag
      421 ccgcgacgcg aggcgaaccg gttgtgggcc gcgatcgccg gagggcgccc gggcgacgcc
      481 tggctgcgcc gggaccgccc gtcggacccg cggacggccg cgcgtttccg ggaggccgtg
      541 cgcgatcgcg cggccggcgt gccgttcgcc tacgcggcgg gcaccgccgc gtttcgcgcg
      601 ctcgagctgg cgatcgaccg gcgggcgctg atcccgcggc ccgagacgga ggggctggtc
      661 gatctggtgc tgcggtggtg ccgggagaac ggcgagtggg gagtggggag gtggggcatc
      721 gcggcggaca tcggaaccgg cagcgggtgc atcgccttgt ccctcgccgt cgaaggggcg
      781 ttcgagcgag tggtcgccac cgatcagtcc gaagcggcgg tgtcgctggc gcgcgagaac
      841 gtggcacggg tcgcaccgtc cgttcccgtg gaagtccgcc tcggcgattt gctggcgccg
      901 ctgggcgacg cgcaccaccg ggtcatcgtc gccaatccgc cgtacctcac cgaggacgag
      961 tggaccgcgc tcgagcccgg ggtgcgcgac tgcgagccga agagcgcgct cgcatcgggc
     1021 gccgacggcc tcgaggtgac gcgacggctg ctggccggcg cccgcgccgt cctggagccg
     1081 gcaggattgc tggccctgga aatcgacgag cggcgagccg ctgcgatccg gcagctcgct
     1141 gaggcgacgg gatggcgcgt gagcattcac cgggacgtgt tcgaccggcc gcggtacgcg
     1201 ctggcggtct gaccgtggag gactctgtga tcgacgagaa ggcgcaggac ctgggacggc
     1261 tgatcgggca gaccgaggag tacaaggccc tgaagcgggc gagtgagcgg ctgcgcgagg
     1321 acgccgactg tcagcggctg ctcgccgaca tggagaagct cgccggtacc atcgagacgg
     1381 ccgcccggga cggcaaggag ccgacccccg agcaggccga gcagtacgac aaggcggtgc
     1441 aaagcattca ggtgaaccag gtgtatcagc aaatggccgt ggcccaggcg aacttcgaga
     1501 agctcatggc caaggtgaac gcccgcatct acgaaggcat ccaaaagggt gcggccagcc
     1561 cgatcatcac cctggggtga tcaccgtggc ggggacgttc gtggtggtgg aggggccgga
     1621 gggcgcgggg aagtcgacgc tggtgcgtgc gctgggggcg cgcttgctgg cggaagggat
     1681 gcaggtgctc ctggtccggg agcccggtgg aacccccgtc gccgaagcgg cgcgcaaggt
     1741 ggcgctgcgc tcgcgacacg agttatcggc ggccgcggag ctgtttctga ttctcgcggc
     1801 ccgggccgac ctggtggccc aggtgatccg gcccgcactc gatgcgggtc aggtggtgct
     1861 ggccgatcgg ttcgatctgt ccacgcgcgc gtaccaggtg gccggccgcg ggctgccggc
     1921 gcatgatgtg gacgcggcat tgcgattggc gacgggcggg ctcgtgcccg acctcacgtt
     1981 ggtgctggac attccggtgg acatcggccg ggaacgtcag cgtaaggcac ggaaggtgca
     2041 ggacagattc gagcgggagg acgacgcgtt tcacgagcgg gtcctggcga cctaccggaa
     2101 cgccgcgggc cctggcgtgg tccatctcga cgcaacgccg tccaagagaa cggtgctgga
     2161 cgcggcctgg cgggagctgc aggcggtgac ggtactatct acgcgaggga tctcatgagt
     2221 cgcaaatggt tgctcgtctc cgtcatgacc ggggtcgcgc tggtaaccgg cggctggttg
     2281 ctgcagcgcg atagcagtcc gcagggcacc gtgtaccagc aggcacgcct gttcgacgac
     2341 gtgctgtcac gggtcgccga tttctacgtc gattcgatcg acgagcggaa gctctacgag
     2401 atggcgatcg acggcatgct cgaccagctg cacgatccgt attcgacgta cctcaagccg
     2461 agcgattttc gctccctgaa cgagcagacc accggcgact acggcggtct cggactccag
     2521 atcgaggtgc gcaacggctg gatcacggtg gtcgctccgc tgcccgacac cccggccgag
     2581 cgggccggca tacagaccgg agacgaaatc gtcacgctcg acgggcggtc cacggagggc
     2641 tggaagcagg acgaggcggt gaatcagctg cgcggcacag cgggctcgac ggtggagctc
     2701 aagattcgtc ggatcggcgt cgaggagccg atcacgttca agctcacccg ggccaagatc
     2761 cacattcgct ccgtccaggt gtccatcatg ctggacgatc gcgtcggcta tgtctcgctc
     2821 aatccggtca gccagacctc ggcggacgaa gtggcgcagg cggtcgacag cctgatcgcg
     2881 aagggcatga agtcgctggt gttcgatctg cgcggcaacc cgggcggctt gttgacgcag
     2941 ggaatcgccg tcagcgacct gttcctcgac ccggggcagg agatcgtcgc gacgcggggc
     3001 cgggcgcccg acgcgtcgca tacctaccgc gacagccggc cgcagcgctg gcccaagctg
     3061 ccgatcgtga cgctggtgaa cgccggcacg gcgagcgcgg cggagatcat cgccggcgcg
     3121 ctgcaggatc acgatcgcgc gctggtcgtc gggacgccta cgttcggcaa gggcctcgtg
     3181 cagtcgctgt acatgctctc tccccagacg gccctgaagc tgaccacagc ccgctggtat
     3241 acgccgagcg gacggaccat ccagcgcgcc agccggagcg aagcggagca gctggcggcg
     3301 ctcgaggcgg cggcgcatgg cacggatacg ctgtccgaca gcgcaccggt gttccacacc
     3361 gacgccgggc gcagcgtgcg cggcggcggc ggcatccggc ccgacatgtt cgagcgaccg
     3421 gacaccttca ccacggccga gcgcgccttc gtgcgggggc tggggagcaa cgtggcgacg
     3481 ttccgcgatg tgctcacggg gtacgctctc gagctcaagt cggggcacgc cctggcatcc
     3541 gcgcaattcg ccgtgacgcc ggcgatggta gacgaggtga tccggcggat gcgccagcgc
     3601 ggcgcggccg tgtccgacac gctcgcggag ggcgcacggt ccctgattcg cgacgagctc
     3661 gggtacgagg cgacgcgcta tgtcttcggt cggggcgcgg aagtgctgcg ccgcatgcac
     3721 gacgaccatc aggtgcagca ggcgctcgac ttcgcccggc gcgcccgcgc accgcaggat
     3781 ctcttcgccc tcgtgacccc gggcggagcg cagcagcgca gcccgtgacc ccgggaccgg
     3841 ggccggctcc cctcgttgtg ggggtagtcg gccccggccg agccggcacc ggcctggcgc
     3901 tcgcctgggc gcaggccgga cacgacgtcc gtctgcatgg gcggcacccc aagccggtcc
     3961 caccccccct cacgttgacg gtcggcgccg cgaacgagcc gccgccgtgg ctcgcggccg
     4021 tctcggtggt cgtgctcgcc gttccggacg acgccatcgc cccgctcgcc cgctcactcg
     4081 cgcgggccgg ctccctcgcc gcgcatcagg tcgtgctgca cctctcgggc ctcctcgatc
     4141 acgaggccct cgccgcgctc gcagccagcg gggccgcgct cggctcgctg caccccctgc
     4201 agacgctggt cgaggccgag caggtgccca tgcatctgcg tggtgcctgg gcggctgtcg
     4261 aaggcacgcc gcccgccgtc gcgacggccg agcgcctcgc ccgcgacatc ggcctgcgcc
     4321 ccttccgggt gcgccccgac gataaggccc gctaccatgc gggcgccgtc ttcgcctcga
     4381 actacttcgt ggtcgtggaa gcggtcgccc aggagctgtt gcggtccacg gggctgagcg
     4441 agaccgatgc ctggagtgcg ctcgccccgc tggtcgccgg cacgctcgac aacctcgcga
     4501 ggcggggccc ggtggccgca ctcaccggtc ccgtggcccg cggcgatgcc gagaccgtga
     4561 cccgccacct ggccgtgttg tccggggacc agcgcgagct ctatcgacgg ttgggaacgc
     4621 tggccctcga cctcgcgcga cggcgcggtc tcgagccggg ggccgcggcg cgcgtggcgc
     4681 gcgcgctggc tactgatcca ccacccggac gccccgggga gcgctgaacc ggaattccct
     4741 ggacggcacc gccgcgttcg cctgcaggtt gctgaggcgg accgagcgct cctgcccgct
     4801 cgtctcgacg agatccaccc ggcgcacgag tccgtcaccg cgtcccaccc agatcaccgc
     4861 ggacgagtag ggctgggagt cgttccgtgg ggtcagggcg atcacatccg cccagcctgc
     4921 cgcactcgaa tccgcccgaa cgtaccgcgc cgtgaaccgc tcgcgggggc gttcgacgaa
     4981 ttgcccgatg agattcggcc cggtgccggt ccgcgggatg cggctgcgga tcacctgccc
     5041 gggctcggtg ctcggtgtgt agagccagag gtagcggccg tcggcgacga tccggtcgcc
     5101 ggccggtgag gtgaaccgca tcgcgaagcg gctggggcgc tgctggaaca gacggccacg
     5161 ggtcgtgtcg ggctcgccca ccatcggatt ggtgatgatc tgcacgaagt tggcggcgag
     5221 ggtctggacg ccctgatagg ccgcgctggc gtggtcgaga atcggccagg gatcctggtc
     5281 ggataggcgg atggatggat agacggatgg tgcgggcagc gcaacgagga gcgcgacgcc
     5341 cagggccatc cgtccattcg ccaatccgtc catccgccta cgctcccacc gccgccggct
     5401 cggcgatgcg ccggccgatg acctcggcga tgacgcggac ctcgcgcatc agctgggcga
     5461 actggtccgg aaagagcgac tgcgccccgt cggacttggc ggcttcgggg tcgggatgca
     5521 cctcgaccat cacgccgtcc gctccggcgg cgacggcagc gcgcgccatc gggatcacct
     5581 tctcgcgcaa tcccgtggcg tgggacgggt cggcgatgat cggcagatgc gagagcttct
     5641 ggacgaccgg gatggcgctc aggtcgaaga tgttgcgggt cgcggtgtcg aacccgcgga
     5701 ccccgcgctc gcacaggatc acgtcgtggt tcccctcggc gagcacgtat tcggccgaca
     5761 gcagcagctc ggtgatcgtg gccgagatgc cgcgcttcag cagcccgggc ttgccggcgc
     5821 ggcccgcggc gcgcagcagc gagaaattct gcatgttgcg cgccccgatc tgcacgatgt
     5881 ccgcgatttc cgacacccac ccgacgccgt ccgggtccat cgcctcggta cagatcaaca
     5941 ttccggtttc cgcgcgcgcc ttggccagca gccgcagccc gggccgcccc agtccctgga
     6001 acgaataggg tgacgtgcgg ggcttgaacg cgcccccgcg caggatgacg gcgcccgcat
     6061 ccttcaccag acgggcggcc tggacgatct gctcctccga ctccaccgag cacggcccgg
     6121 cgatcacgac gatgtcgttg ccgccgacgg cgatgccgcc gggcagccgg accacggtgt
     6181 tctcgggttg ccactcccgg ctcacctgct tgtagggctt ggagacgtgg atcacgtcct
     6241 ggacgccggg cagtccctcg agacgggaag attccacccg gccgtcgttg cccacgaggc
     6301 cgaccgcggt tcgctgcgcc ccgggcatgg gacgggcctg gtagcccatg tcccgaatca
     6361 ccttgacgac ccgttggatt tcctcttcgg tcgccccgtg gcgcatcacg atcagcacga
     6421 cgtcttctcc tgcgcgatag tgaaccgttc gccgtccacc gcggcggtga ccgggccgcc
     6481 gaagcggctg gcggcgcgtt gggcgggatc ggaattctcg actgaaggat agaagtgcgt
     6541 caagacgagg cgtttcgccc cggcagcgcg ggccagttcc cccgcccgtt ccggggtcaa
     6601 atggatgtcc agcgccatcg cctcgggaag cgaacactcc gcgagcagca cggtgcaccc
     6661 ggtggcccac cgtgccagct cgggggaggg ccccgtatct ccggtataca ccaggcggcc
     6721 ctcaggtgcc gtgaggctga aggcgacact ctcctcggtg tgcggtaccg ggaacgattc
     6781 gagcgacagg tcggcatcga gcggaaacgg ctcgcccggc tcgacgtcca tgatcagcag
     6841 cgggaatccc gggccgagga gccacgaacc atatccttcc gcgagcgcct tcaccagacg
     6901 cacgacgccg cgcggcccca gcaccaccaa cggctcgctc cggggcggca ccgtcgtgta
     6961 cttcagcgcg tacacgagca tcggcagatc ggcgaaatga tccggatgga agtgcgacag
     7021 ggcgacgtgc gtgatgtcgc tccaggggag cccgaactgg gcgagccggt gcagggttcc
     7081 gggcccgcaa tcgagcagcg cccgcacctc cccccgctcg acgtaatggg cggcggcggt
     7141 ccgtcgcgcc gaaggcgcga ccgttcccgt tccgacggtg atcagctcca cggcgtcacg
     7201 atgtccttcc ccggtacacg atacgcttga attgaataac gccgccgaac ttcaacttgt
     7261 acaggtagac cccggcgggc agcc
//
LOCUS       DBNGZW010000410         1791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1880691, whole genome shotgun sequence.
ACCESSION   DBNGZW010000410 DBNGZW010000000
VERSION     DBNGZW010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1880691"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..503)
                     /locus_tag="AC16JC_09690"
     CDS             complement(<1..503)
                     /locus_tag="AC16JC_09690"
                     /inference="COORDINATES: protein motif:HMM:NF026257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5165005.1"
                     /translation="MTTGRLLFDTLRFHGAPPSERLAAAWAGADLRGLPRLVALEGCA
                     IWVYRRLRQIGALEQMDHDLADWLASQATDQTARNLLIDAEADALTEIFQRIGVPAVF
                     MKGVARRLLAERFPLADARSTHDVYLIVPAERAWEVWHELRRLGYERTRLTKPPRPEH
                     HHLPALMG"
     gene            complement(500..934)
                     /locus_tag="AC16JC_09695"
     CDS             complement(500..934)
                     /locus_tag="AC16JC_09695"
                     /inference="COORDINATES: protein motif:HMM:NF033537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lasso peptide biosynthesis B2 protein"
                     /protein_id="HIR5165006.1"
                     /translation="MSGGLPLGWRLRAAAAVLIVPPLLYFTSFARLASWLGGSSSRKV
                     EPGLDDAALARWVDRLLRNLPGPWRRTCLKRSVVLYYLLRRAGRPVELCIGVRNEEPS
                     SVAAHAWLVRGGAPYLEARPEHAGRHTVIARFPETRAAAARS"
     gene            complement(931..1206)
                     /locus_tag="AC16JC_09700"
     CDS             complement(931..1206)
                     /locus_tag="AC16JC_09700"
                     /inference="COORDINATES: protein motif:HMM:NF017239.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HIR5165007.1"
                     /translation="MSERYLRHPDLRLADVEGDGVVLHLGTRRYFSVSESGLALLQAL
                     ESPCTVPDLVTVLLERYEVTPDQAEESVLQFLETCRTADLLAPEAGG"
     gene            complement(1203..>1791)
                     /locus_tag="AC16JC_09705"
     CDS             complement(1203..>1791)
                     /locus_tag="AC16JC_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165008.1"
                     /translation="AVVILLLRRVGWYHLHAATAIDPGGQGWLFTGNAHVGKSTTAAL
                     LATSGWQVGTDDAAFLERVTDRILVHTAHAPIALREGGRRLLAKAGGIVLPDRGKVGF
                     WPEDLGAVWTPFVSPDIVIFPAVGRTRTTAQPIDRAEALAELVRWSAWVMLEPDLAQA
                     HLDVLTQLAGQARCYRVTLGRDLFARPNRLIELVT"
BASE COUNT          315 a          656 c          591 g          229 t
ORIGIN      
        1 cccatcagcg cgggcagatg atggtgctcg ggccgcggcg gcttggtcaa tctggtgcgc
       61 tcgtagccga ggcgacgcag ctcgtgccac acctcccacg ctcgctcggc cgggacgatg
      121 aggtacacgt cgtgcgtgct gcgggcatcg gccagcggaa accgttccgc caggagcctg
      181 cgggcaacgc ctttcataaa gaccgcggga accccgatcc gctggaagat ctcggtcaac
      241 gcgtcagcct cggcgtcgat cagcaggttg cgggcggtct ggtcggtcgc ctgcgaggcg
      301 agccaatcgg cgaggtcgtg atccatctgc tccagtgcac cgatctggcg cagccggcgg
      361 tagacccaga tcgcacaccc ctcgagcgcg accagccggg gaagaccacg gaggtcggcg
      421 ccggcccatg ccgccgccag acgttcggaa ggcggcgcgc cgtgaaaccg gagcgtatcg
      481 aacagcagac gaccggtggt cacgaacgcg ccgcagccgc tcgtgtctcg gggaagcgtg
      541 cgatcaccgt gtgccgaccc gcgtgctccg gccgagcttc gaggtacggc gcgcctccgc
      601 gcaccagcca ggcgtgcgcc gccaccgacg acggctcctc gttgcgcacc ccgatgcaca
      661 gctccacggg gcgcccggcc cgccgcagca ggtaatacaa caccaccgat cgcttgaggc
      721 aggtgcggcg ccacgggccg gggagattgc ggagcaaccg atccacccag cgtgccagcg
      781 ccgcgtcgtc gagtccgggc tcgaccttcc gggaggagct cccgcccagc cagctcgcaa
      841 gccgcgcgaa cgaggtgaaa tacaagagcg gcggcacgat cagcaccgcc gccgccgccc
      901 gcagacgcca gcccagcgga aggcctccac tcacccgccg gcctccggcg cgagcagatc
      961 ggcggtacgg cacgtctcca ggaactggag gacgctctct tccgcctggt ccggcgtcac
     1021 ttcataccgc tcgagcaaca ccgtcacgag gtccggcacg gtgcagggcg actccagcgc
     1081 ctggagcagc gccaatccgc tttcgctcac ggagaagtag cggcgggtcc cgaggtgcag
     1141 gaccaccccg tctccctcca catcggccag gcgcaggtcc ggatgacgca ggtagcgttc
     1201 gctcatgtca ccagttcgat cagccggttc ggccgggcaa acaagtcgcg gcccagcgta
     1261 acgcgataac agcgcgcctg cccggcgagc tgcgtcagca catccaggtg cgcctgggcc
     1321 agatccggct ccaacatcac ccacgccgac cagcgcacca gctcggcgag tgcctcggcg
     1381 cgatcgatcg gctgcgccgt cgtccgcgtc cttccgacgg ccgggaaaat aacgatgtcc
     1441 ggcgaaacga agggggtcca aacagcaccc agatcctccg gccagaaccc gactttgccg
     1501 cgatcgggaa gcacgatgcc gccggccttc gccaggagac gccgcccgcc ctcgcgcaac
     1561 gcaatcggtg cgtgggcggt gtgcaccaga atgcggtcgg taacgcgctc gagaaatgcg
     1621 gcatcgtcgg tcccgacctg ccagccggag gtcgccaaca gcgcggccgt cgtcgacttc
     1681 cccacgtgcg cgttgccggt gaacagccag ccctgcccgc ccggatcgat cgccgtcgcc
     1741 gcgtggagat ggtaccaacc cacccggcgc agcaacagga tgacgacggc g
//
LOCUS       DBNGZW010000411         3186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_516995, whole genome shotgun sequence.
ACCESSION   DBNGZW010000411 DBNGZW010000000
VERSION     DBNGZW010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_516995"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..733
                     /locus_tag="AC16JC_09710"
     CDS             179..733
                     /locus_tag="AC16JC_09710"
                     /inference="COORDINATES: protein motif:HMM:NF028519.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdrD B-like domain-containing protein"
                     /protein_id="HIR5165009.1"
                     /translation="MRVPVVLFALVTTPFLAGAAQSAAVKDPAKCATADANRSATSLS
                     QDRPLDPFGRPRTGCAPVAPAPTGGGGEDPGGGGTGGGTGGGTGTPVISGTVWNGSSW
                     LGMGGWVIEVTGTATASTVTNADGTYGFWNLPAGTYTVCEQVKSGWTQTYPTNGSPCP
                     TGQGYTFTLAAGSSGSFLDFVDIQ"
     gene            complement(823..1902)
                     /locus_tag="AC16JC_09715"
     CDS             complement(823..1902)
                     /locus_tag="AC16JC_09715"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5165010.1"
                     /translation="MSYAHFMSLIMLTLGGIPAFFDRLPGDPPAVSVRLSEWKVELSE
                     PTVAAGAVTFTIANTGTIPHAFEIEGQGIERETAVIQPGASATLTLQLKPGSYEVYCP
                     VGQDSHKKLGMDARLRVEGSGGESGSPAMEESHAMGGSTAVPGIQVTGGGPVIQILPG
                     PFAFPDSAAPILASFGNEREGLESQIQNGPYSNNVASSAGTFTFSAWDKGAVRDSVDG
                     TAEFTTRDGTRWKLVLDRVQTKDVPHHPRFGGVIMGLYYHGNTGVHTPLVPTINSAVA
                     LWAVGHLYRNGQLVSDNAQTHVMLLSHTRRAGDFALACWDCSKNKVDELQLQILPGPG
                     EAKFDAPGGFLFLNWEKSGSRKLGV"
     gene            complement(2049..3002)
                     /locus_tag="AC16JC_09720"
     CDS             complement(2049..3002)
                     /locus_tag="AC16JC_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012631192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HIR5165011.1"
                     /translation="MSPRRDRFALSALLLSQLVLGCEKAKPDDRAEAQQRPPLEPSAM
                     DWAAVGQALGKAGGMQPGNVYKVGFPRSDLHVTVGRVTVRPALALGSWLAFRQTADSE
                     AMVLGDLVLLESEVALVMSKLEGSGIRATALHNHLLHESPPIRYLHIMGRGAAAKLGA
                     GVRAALDLTGTPLAAAAATPAAAAPLGLDTALIAQALGYQGNVNGGVYQVGVPRTDTV
                     TVDGFVIPPAMGVATAINFQPTGGGKAAVTGDFVLVVAEVNPVIQALRATDIEVTALH
                     SHMLGDSPRLYFMHFWANDDAVKLARGLGTALAKMNVRKAG"
BASE COUNT          480 a         1080 c         1078 g          548 t
ORIGIN      
        1 gagccacgtc acatgccgtg cggcaggacc ccggcgccgc ggcgtcgacg ccgcgcggcc
       61 atccgcaagt ctcgtgtgcg tctcgtgttc ctttgccgcg tgatgcgcga cacggcttcc
      121 cgctcgccgg agtgcgattc tgtcgcgcgc ctgatcgtgt acctaacagg agatctcgat
      181 gcgtgtgcct gtagttctct tcgccttggt gacgacaccg ttcctcgcgg gagcggcgca
      241 gagcgcggcc gtcaaggacc ccgcgaagtg cgcgacggcc gacgcgaatc gttcggcgac
      301 gtcgttgtcg caggaccgcc cgctcgatcc gttcggccgt ccccggacgg gatgtgcccc
      361 cgtggcgccc gcaccgacgg gtggaggtgg ggaggacccg ggcgggggcg ggaccggggg
      421 tgggaccggt ggcggaacgg gtacgcccgt gatctccggg acggtttgga acggcagcag
      481 ctggctgggg atggggggat gggtgatcga agtcaccggc acggcgacgg cctcgacggt
      541 caccaatgcc gacgggacgt acgggttctg gaatctgccc gcgggcacct acaccgtctg
      601 cgaacaggtc aagtccggct ggacccagac ctatccgacg aacggctcgc cctgtccgac
      661 cggccagggg tatacgttta ccctggccgc cggcagcagc ggcagcttcc tcgacttcgt
      721 ggacatccag tagcagacgg ttccggctcc atcgggctgg aacgaaccga gtcccgcatc
      781 ctcatcagtc gaggatgcgg gactcttctc gttcgccgcc gctcagacgc ccagcttgcg
      841 agatcccgac ttttcccagt tgaggaacag gaacccaccg ggcgcgtcga acttggcctc
      901 gcccggcccg ggcaggatct ggagctgcag ttcgtccacc ttgtttttcg agcagtccca
      961 gcaggcgagc gcgaaatcgc cggcccgccg cgtgtgcgac agcagcatca cgtgcgtctg
     1021 ggcgttgtcg ctcacgagct ggccattgcg atacaggtgc ccgactgccc acagcgcgac
     1081 ggcgctgttg atggtgggaa cgagcggcgt gtgcacgccg gtgttgccgt ggtagtagag
     1141 acccatgatc acgccgccga agcgggggtg atgcgggacg tccttcgtct gcacccggtc
     1201 caggacgagc ttccagcgcg tgccgtcgcg ggtggtgaac tccgcggtcc cgtccaccga
     1261 gtcccgcacg gcgcccttgt cccatgcgga gaaggtgaac gtccccgcgc tcgacgcaac
     1321 gttgttcgaa tacgggccgt tctggatctg ggactcgagg ccctcgcgct cgttgccgaa
     1381 cgacgcgaga atcggcgcgg cgctgtcggg gaacgcgaag gggcccggaa ggatctgaat
     1441 gacgggccca ccgccggtca cctggatgcc gggcaccgcc gtgcttcccc ccatcgcgtg
     1501 tgactcctcc atcgcgggac tgccggattc acccccggag ccctcgaccc tcagccgcgc
     1561 gtccatgccc agcttcttgt gggaatcctg acccacgggg cagtacacct cgtagctccc
     1621 cggcttgagc tggagggtga gcgtggcgct ggcgcccggc tggatcaccg cggtttcccg
     1681 ctcgattccc tgaccctcga tctcgaaggc gtgcgggatg gtgccggtgt tcgcgatggt
     1741 gaaggtcacc gctcccgcgg cgacagtggg ctccgagagc tcgactttcc actcggacaa
     1801 cctgacgctc acggccgggg gatcgcccgg caggcggtcg aagaaagcgg ggatccctcc
     1861 cagggtgagc atgatgaggg acatgaagtg tgcgtacgac atgagagtct atcctccggc
     1921 gaagagtggg cagcattcat cgttcactgc cggagactcc ccgccggacg ggggtattcc
     1981 cgcgggaata aactcgtcgc tggtgaggtc ctgttcctcc agggccgcga ggcgacaggt
     2041 ccacgcagtc atcccgcttt gcggacgttc atcttcgcca gcgcggtgcc cagcccgcga
     2101 gccagtttga ccgcgtcgtc attcgcccag aaatgcatga agtagagtcg cggcgagtcg
     2161 ccgagcatat ggctgtgcag cgccgtcacc tcgatgtcgg ttgcgcggag cgcctggatc
     2221 accgggttga cctccgcgac gacgagaacg aagtcgcccg tgacggccgc tttgccgccg
     2281 ccggtcggct gaaaattgat cgccgtcgcc acgcccatgg ccggtgggat cacgaatcca
     2341 tccaccgtca ccgtgtccgt ccgcggcacg cccacttgat agacgccgcc gttcacgttc
     2401 ccttggtacc ccagggcctg ggcgatcagc gccgtatcga ggccgagcgg cgcggccgcc
     2461 gcaggtgtgg cggcggcggc agcgagtgga gttccggtca ggtcgagcgc cgctcgcacg
     2521 ccggccccga gcttcgccgc cgccccgcga cccatgatgt gcaggtagcg aatgggggga
     2581 gactcgtgga gcaggtgatt gtgcagggcg gtggcccgga tgccgctccc ttcgagtttg
     2641 ctcatgacca gggcgacttc gctctcgagc agcacgaggt cgcccaggac catcgcctca
     2701 ctgtcggccg tctgccggaa tgcgagccag gagccgagtg ccagtgccgg ccgcaccgtc
     2761 acccggccca cggtgacgtg gagatcactc cggggaaagc cgaccttgta gacgttgccg
     2821 ggttgcatcc ctcccgcctt gccgagcgcc tggcccaccg ctgcccaatc catggccgac
     2881 ggctcgagcg gcgggcgctg ctgcgcttcc gctcgatcgt ctggtttcgc cttttcacat
     2941 ccgagcacga gctggctcag cagcagggcg ctgagcgcga agcggtcccg acgcggtgac
     3001 ataccttctc cctgtgggag tggtgaggat tgcttgctgc cacgatacgg tatcgataca
     3061 cttgtgacct gcgagtgact gacccgcgtc acgcccctct ccttctcgcc gccaccctgg
     3121 ccttcagcgc cgggcttgcg ggccagacga cctctctccg tgtcgccgct gcgacggtcc
     3181 ccgtcc
//
LOCUS       DBNGZW010000412         2604 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_242590, whole genome shotgun sequence.
ACCESSION   DBNGZW010000412 DBNGZW010000000
VERSION     DBNGZW010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_242590"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="AC16JC_09725"
     CDS             <1..285
                     /locus_tag="AC16JC_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HIR5165012.1"
                     /translation="EEAVEGAHVVNTDVWASMGQETEAEARRNAFRGYTVDAELMKLA
                     DPGAIFLHCLPAHRGEEVSAEVIEGRQSHVWDEAENRLHVQKALLATLMG"
     gene            296..1411
                     /gene="gcvT"
                     /locus_tag="AC16JC_09730"
                     /pseudo
     CDS             296..1411
                     /gene="gcvT"
                     /locus_tag="AC16JC_09730"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
     gene            1408..1911
                     /locus_tag="AC16JC_09735"
     CDS             1408..1911
                     /locus_tag="AC16JC_09735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HIR5165013.1"
                     /translation="MTRAGTPLRIGVLTVSDLGAQGARADTSGDAIVTWSTARGYDIT
                     VRSVVADETGRIAGKLARWADSGEMDVILTTGGTGLTARDVTPEATSAVLERLAPGIA
                     EALRARAAPDFPRAWLSRGVAGTRGRTLIVNLPGSPGGVQDGLAVLEDLLDHAVALLR
                     GQGSDHS"
     gene            1918..>2604
                     /locus_tag="AC16JC_09740"
     CDS             1918..>2604
                     /locus_tag="AC16JC_09740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HIR5165014.1"
                     /translation="MADRVLVTTDDLEYAVRVNGALEGAGFATAMVTSFDDARDALTG
                     REPDCLVVTGALHEPATARLLTAARDRAVSTLGLVEETEPDPKDLAAELGLTAFLEKP
                     ADAGEVARLVRRLVDRRRLQQRTGIIGESPAIQEVLVKIEQMAPVSSTVLVEGESGTG
                     KELVARAIHDLSPRRERPFITVNCAALPETLLESELFGHEKGAFTGAHARKLGRFELA
                     HGGTVFLDEIG"
BASE COUNT          413 a          917 c          916 g          358 t
ORIGIN      
        1 gaggaggcgg tcgaaggggc gcacgtcgtg aacacggacg tctgggcgtc gatggggcag
       61 gagaccgagg cggaggcccg ccgcaacgcg ttccgcggct acaccgtgga cgccgagctg
      121 atgaagctcg ccgatccggg cgccatcttc ctgcactgcc tgcccgccca ccgcggcgaa
      181 gaagtctccg ccgaggtcat cgagggccgg cagtcgcacg tgtgggatga agcggagaac
      241 cggctgcacg ttcagaaagc actgctcgcc accctgatgg gatgacaccg gaaccatgag
      301 tgacacgacg ccgtccaccg cgctcaagcg cacgccgctc cacgacctcc acgtggcgct
      361 gggggcgaag atggtgccct tcgccgggta cgaaatgccc gtgacctacc cggccggcat
      421 cggcgccgag caccgggcgg tgcgcgagcg cgtcggcgtg ttcgacgtct cgcacatggg
      481 tgaattcgag gtcaccgggc ccgaccgcaa cgcctttgtg cagcgcgtca cctgcaacga
      541 cgtcgcggcg ctgcggaccg ggcaggccca gtactcgggg atcctgacgg agtaggggac
      601 gttcgtcgac gattgtctcg tgtaccgctt cgacgacaag gtgatgctcg tggtcaacgc
      661 ctcgaacatc gccaaggatt gggagcacat cgtcgcccag aagggcggcg ccaacgtccg
      721 gctgcgcgac atctcggacg agaccgccct gctcgcgatc caggggccga atgccgaagc
      781 gctggtggcg gccctctcgc ccgtcgcggt ggcgcagatc ccgtactacc acttcacctc
      841 cgggatggtc tcgggcgcgc agtgcttcat ctcgcgcacc ggctacaccg gagaagacgg
      901 cttcgagctc tactgccgcg ccgccgacgc gccgcggctg tgggaggcga tcaccgggcg
      961 cggcgccgag ccgtgcggct tgggggcgcg cgacacgctg cgcctcgagg cggggttgcc
     1021 gctgtacggc aacgacatcg acgacaccac cacgccgctc gaggccgggc tcggattcat
     1081 cgtcaagctc gacaaggagg cgcccttcac cggccagccg aggctgaagc ggcaaaagct
     1141 cgaggggatc cagcgccgcc tggtgggatt ccgcgtgacc gagccgaagc tcgtggcgcg
     1201 ccacggcttc ccggtgtcgc tcgacggcaa gcccgtcgac atcgtgcgca gcggcacggt
     1261 cacaccgacg gtgaacgccg ccatcggaac gacctacctg ccgaccgcgc gcgccaaagc
     1321 cggcacgacc ttcaccatcg acgtgcgtgg ccgcccggcg cccgcggagg tcgtgaaatt
     1381 gccgttcgtg ccgcatcgga ccaagcggtg acgcgtgcag ggacgccgct gcggatcggt
     1441 gtgctgaccg tgtccgacct cggcgcccag ggggcgcgcg ccgacacctc cggcgatgca
     1501 atcgtaacat ggtctacagc aagaggttac gacattaccg ttcgcagcgt cgtggccgac
     1561 gagacggggc gcatcgcggg caaactggcg cgctgggccg attccggtga aatggacgtg
     1621 atcctcacga ccggcggcac tgggctcacc gcgcgcgacg tcacgcccga ggccacgagc
     1681 gccgtgctcg agcggctggc tcccgggatc gccgaagcgc tgcgcgcccg cgccgcgccg
     1741 gactttcccc gggcgtggct gtcgcgcggc gttgccggca cccggggcag gacgttgatc
     1801 gtcaacctcc cgggctcgcc cggcggcgtg caggacggcc ttgcggtgct tgaagatctg
     1861 ttggaccacg cggtcgccct gctgcgcggt cagggcagcg accacagctg acggctgatg
     1921 gccgaccgcg ttctcgtcac caccgacgat ctcgagtacg ccgttcgcgt gaacggagcg
     1981 ctcgaagggg cgggcttcgc gacggcgatg gtcacgtcgt tcgacgacgc ccgcgacgcg
     2041 ctcacgggac gcgagcccga ctgcctagtc gtgacgggcg cgctgcacga gccggcgacg
     2101 gcccggctgc tgacggccgc gcgcgaccgg gccgtctcca cgctgggcct cgtcgaagag
     2161 accgagccgg atcccaagga tctcgccgcc gagctgggcc tcaccgcctt cctcgaaaag
     2221 ccggccgacg cgggcgaagt ggcgcgcctg gtgcggcgcc tggtggaccg ccgtcgcctg
     2281 cagcagcgca ccgggatcat cggcgagtcg cccgccatcc aggaagtcct ggtgaagatc
     2341 gagcagatgg cgcccgtcag ctcgaccgtg ctggtcgaag gcgaatcggg caccgggaag
     2401 gagctcgtcg cccgcgccat ccacgacctc tccccgcggc gcgagcggcc cttcatcacc
     2461 gtcaactgcg ccgcgttacc cgagaccctg ctcgaaagcg agctgttcgg gcacgagaag
     2521 ggcgcgttca ccggcgccca cgcccgcaag ctcgggcgct tcgagctcgc ccatggcggc
     2581 acggtgttcc tggacgagat cggc
//
LOCUS       DBNGZW010000413         2068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_436670, whole genome shotgun sequence.
ACCESSION   DBNGZW010000413 DBNGZW010000000
VERSION     DBNGZW010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_436670"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1623)
                     /locus_tag="AC16JC_09745"
     CDS             complement(<1..1623)
                     /locus_tag="AC16JC_09745"
                     /inference="COORDINATES: protein motif:HMM:NF051698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR5165015.1"
                     /translation="MIRVRIVPLFMLLSAPLVAPRAQGVSTASIGGVVIDAGGRAIEA
                     ARVRVVNVATGVGITGESGRGGRYAVDGLAVGGPYTVTVERIGYQPGRRDGLMLLLGQ
                     ALRLDFVLEAVATELPPIMVQADAESRFAIAVSTGTQVSDSALRRLPTVNRDLYSFAQ
                     LTPQIISRSGSSAAGSNSLFNGIRVDGANLQAFWGGNSAGGPVWGGKPISIEAVREYQ
                     VLLAPFDVRQGDFAGAQIIAVTRSGTNDWRGTAFYYWRNETLARNVSYLRASPNERSQ
                     FGFALGGPILRDRVHFYVASELQRLSAPAAGPYVGQGAGSQTPLSVDTADINRFNQLL
                     AAHGLEGGSAGPVSNTNPSVNLFARMDVALPRWNTRLTLRHNFARADSNAFSRPTAVS
                     TACGQPTCFPLSSVGRAHRTTQHSTALHVLTNFRSGAYNEATLGRLAAPFGITPNVRA
                     PMIVVTVPRADAPGTPRLQAGSIETGQVYDISQVGYEASDNFTLPLGAHRVTLGVASL
                     WYHLRAAQVRDAYGVWRFSNLDSLAVNAAEAYQ"
BASE COUNT          346 a          697 c          724 g          301 t
ORIGIN      
        1 ctggtaggcc tccgccgcgt tgaccgccag ggaatccagg ttggagaaac gccacacgcc
       61 gtacgcgtcc cgtacctggg ccgcgcgcag gtgataccac aggctcgcga cgcccagggt
      121 gacacggtgg gcgccgagcg gcagcgtgaa gttgtcgctc gcctcgtagc ccacctggga
      181 aatgtcgtat acctggccgg tctcgatact gccggcctgc aagcgcggag taccgggggc
      241 gtcggccctc ggcaccgtca cgacgatcat cggcgctcgc acattgggcg tgatcccgaa
      301 cggcgccgcc aggcggccga gcgtcgcctc gttataggcg ccgctccgga agttggtcaa
      361 gacgtgcagg gcggtcgagt gctgcgtcgt gcggtgagcc cggccgaccg acgagagcgg
      421 gaagcaagtc ggctgtccgc aggcggtgga aaccgcggtc gggcgcgaga acgcattgct
      481 gtcggcacgg gcgaagttgt ggcgcagcgt gagacgagtg ttccatcgcg ggagcgcgac
      541 atccatccgc gcgaacaagt tgaccgacgg gttggtgttg ctgacgggcc ccgccgaccc
      601 gccctccaga ccgtgtgccg ccaacagctg gttgaagcgg ttgatgtcgg ccgtgtcgac
      661 cgacagcgga gtctggctgc cggcgccttg ccccacgtag gggccagcgg ctggcgcgct
      721 cagccgctgg agctcgctcg cgacgtagaa gtgcacgcgg tcacgcagga tcggcccgcc
      781 gagggcgaag ccgaactgcg agcgctcgtt cggtgatgcg cgcaggtagg acacgttgcg
      841 ggcgagcgtc tcgttgcgcc agtagtagaa cgccgtgccg cgccagtcgt tcgtgccgct
      901 ccgcgtgacg gcgatgatct gagcgccggc gaagtccccc tgtcgcacgt cgaacggcgc
      961 caacagcacc tggtactctc gaacggcctc gattgaaatc ggcttgccgc cccagaccgg
     1021 cccccccgcg gagttgccgc cccaaaatgc ctgcaggttc gccccgtcca cgcgaatgcc
     1081 gttgaagagg ctgttgctgc ccgccgccga cgaaccggac cggctgatga tctgtggggt
     1141 tagctgcgcg aaactgtaca gatcccggtt gacggtcggc aggcgccgca gcgccgagtc
     1201 cgacacctgc gtccccgtcg aaaccgcgat ggcgaagcgt gactccgcgt ccgcctggac
     1261 cataatgggc gggagctccg tcgccaccgc ttcgagcacg aagtcgagcc gcagggcttg
     1321 gccgagcagc agcatcaggc cgtcgcgtcg ccccggctgg tagccgatcc gctcgaccgt
     1381 gaccgtgtac gggccgccca ccgccagccc atccaccgcg tagcggcctc ctctaccgga
     1441 ctcgcccgtg ataccgacgc ccgtggcaac attgactacg cgaacccgcg cggcctcgat
     1501 ggcccttccg cccgcgtcga tgacgacgcc accgatggac gcggtgctga ccccctgcgc
     1561 ccggggcgcc acgagtggtg cgctgaggag catgaacagg gggacgatcc ggacgcggat
     1621 caccttggcc ttgggggcgg acgggatgac gtgagcgcga gatagcagag aagggccgaa
     1681 cgtctcggct gggccagaac cgacgccaca ggtgtggagt cgtcgcccct cagctccagc
     1741 gcgcccagcg tccgcatcga tcatgcagga ggactcggca cgtggccatc acccggcggt
     1801 gacgctaagg gggtcagccg agtgacgcct accgcgcata ctcgtgcaac ctagggcgac
     1861 gccggacccc gagcaagcgg cagcgaaagc ctcggcctga gcggtgagcc attcgcgcgg
     1921 gattcgtcgt aacaggtaga gaagggaaaa ccggggctcg gacttcgatc ccgcttggtc
     1981 gcccgacggc tcgtacattg cgttctcggg catcggcgac ggcggcaatc cggagatctg
     2041 ggtgatggac gcggacggcc agaacccc
//
LOCUS       DBNGZW010000414         5522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_311097, whole genome shotgun sequence.
ACCESSION   DBNGZW010000414 DBNGZW010000000
VERSION     DBNGZW010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5522
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_311097"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="AC16JC_09750"
     CDS             complement(<1..597)
                     /locus_tag="AC16JC_09750"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR5165016.1"
                     /translation="MGQGASGDTTTLTLAAARQRALAANPALRAERAGARAGAAGPLE
                     ASRAFLPTLIADAQAVRSTDPVAVFGMKLRQSEFTAGDLSLSALNGPAPYTDYTARLG
                     VELPLLNLEGWYGYAAAGRAAAARNAGARRAAGATILAVSRAYWDAQLAAERVAALDT
                     ALGAAYAHAAQAEALHQQGLVSGLDARLARLRAARIESQ"
     gene            complement(667..972)
                     /locus_tag="AC16JC_09755"
     CDS             complement(667..972)
                     /locus_tag="AC16JC_09755"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5165017.1"
                     /translation="MAAKQLLRRIDPAILARAADIIKLLGHPERLKIVEVLERGEASV
                     SEIRDAVRLPQAIVSQHLARLRGANVVAARRDGVHVLYRVIEPKVHHVLTCIRECDT"
     gene            complement(1045..1485)
                     /locus_tag="AC16JC_09760"
     CDS             complement(1045..1485)
                     /locus_tag="AC16JC_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013458750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HIR5165018.1"
                     /translation="MAGGSHVWDRAVHKANAWLQELDGQLGWGDLDATLLALRAVLHA
                     LRDRLTANEAVQLAAQLPLLIKGVYFDGWRPSATPQRMRSREEFLDMVRGPLLRGIPE
                     ADPEDVTRAVFRLLDEHVSGGEISDVRSVLPSEWSDLWPAATRR"
     gene            complement(1507..1944)
                     /locus_tag="AC16JC_09765"
     CDS             complement(1507..1944)
                     /locus_tag="AC16JC_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5165019.1"
                     /translation="MSAGRICSRMVATAWPNEPVRVAARRMAEQEVGTLVVVEGDGAG
                     EAVGMVTDRDIALRCVAGGLDPDRATVAQIMTRPVKAIDEEASIEEAITRMAEKGTRR
                     LIVTGEGKRLVGILSIDDILGLLIEEAGPIGRLLEKQSPRVGA"
     gene            2127..3173
                     /locus_tag="AC16JC_09770"
     CDS             2127..3173
                     /locus_tag="AC16JC_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019247483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5165020.1"
                     /translation="MTALPTRPLGETGLHITPIGMGTWALGGPGWALSWGLQTDAASL
                     GAMHHALRRGINWIDTAPVYGLGHAEELVGRLLKGLPAAERPFVFTKCGMQCDPRDPS
                     REPRRDLRPATIRRECEASLRRLGVKQIDLYQIHWPDEFGLPVEYSWAEMIRLRDEGK
                     VRALGVCNFDAWLLTNCAERCPVDSLQAPFSLIRRAAALTDIPWCVDHGTGVLAYSPL
                     ASGLLSGAFTEARLQRLAADDWRRTAPEFRQPRLGHNLALARALRPIAHRHGTSVAAV
                     AVAWAAHWPGVTGAIVGARAPHQVDDWIGGATLELVAEDLTEMTAAIKHSGAGDGPAS
                     PQDVEAWQWVGVQE"
     gene            3226..3471
                     /locus_tag="AC16JC_09775"
     CDS             3226..3471
                     /locus_tag="AC16JC_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165021.1"
                     /translation="MTAFSHLAATSPAPRRFNVVLPAETFSELNQLASRCGRSPAEVV
                     QTALDLLKVADDEAFRGNVLAIATRDGRPLRQIVIPR"
     gene            complement(3538..4761)
                     /locus_tag="AC16JC_09780"
     CDS             complement(3538..4761)
                     /locus_tag="AC16JC_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165022.1"
                     /translation="MSTRHLRTAAYAAAAASLVVALGGCAERSGPTESRVVASISKEQ
                     VTPTPIVTVTNGDSSLEFWPWTGNDFSGTQEDPINLVLFGQADPRALRAALLLLDGDR
                     SGIGLPDVAPFNCTWHDVPEGTVQTTFGSATGWTGGVIQLACGEYAVRFHMRLFDLGG
                     GTVGGAHFEVQIPGTPEHQVLSWEFAEAIVAFDFSRTGLLGAVSQTGAINTPPPFREI
                     PAVIYNGLLADPNGFQLLALLQYPLSEQSADFPVPNDGTATMMTLDDGVNGEPLVAQQ
                     RFEVEFDQTIPKPICTGETPFDLLLVQGPLTFTQHVVLTPSGNYLSTFQLRGHLDLTL
                     IDGSTGEPVGQPYQALINEQHHAILTDNVTLAASFNMQIEIPSAGPFRGSIVTRVSVG
                     PGNASFSALTVRCGD"
     gene            4908..5477
                     /locus_tag="AC16JC_09785"
     CDS             4908..5477
                     /locus_tag="AC16JC_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165023.1"
                     /translation="MKTPAVLLAALAGAGCASHRVLVPPRLDLAPYNSIGLVTFTVEN
                     ARGGLGDLATRRFEEYMLGAQKGFEVQGFAAADSAAALSGARGVPVVFLGRLKVSNVK
                     PSGALGLVSAHLEATVSAELSVELRSTRTGGTLWRASSTAREKVGQVAVQGGLPDFSA
                     RDPNDAYGRLVDRLVHAVTWDLRSTWVKQ"
BASE COUNT          869 a         1932 c         1842 g          879 t
ORIGIN      
        1 ctgcgactcg atccgcgcgg cgcgcaggcg agcgagtcgc gcatcgagac ccgaaaccag
       61 tccctgctgg tggagtgctt cggcctgcgc cgcgtgggcg tacgcggccc ccagcgcggt
      121 atcgagcgcc gccacccgct cggcggccag ctgcgcgtcc cagtaggcgc gcgatacggc
      181 caggatggta gcgcccgcgg cgcgccgcgc ccctgcgttc cgcgcggccg ccgcccggcc
      241 ggccgcggcg tacccatacc acccctccag attgagaagc ggcagctcga cgcccaggcg
      301 cgctgtgtag tcggtatacg gtgccggacc gttgagcgca ctcaggctca ggtccccggc
      361 cgtgaattcg ctctggcgca gcttcatgcc gaacaccgcc accggatcgg tggagcgcac
      421 ggcctgcgcg tcggcgataa gcgtcggcag gaaggcgcgg gacgcctcca ggggtcccgc
      481 cgcgccggcg cgggcgcccg cccgctcggc gcgcaacgcc ggattggcgg cgagggcgcg
      541 ctggcgtgcc gcggcgaggg tcagcgtcgt cgtgtcgccc gacgcgccct gtcccataat
      601 cgatagtaaa atagctatta gcatatgtat ggcgcacgac tcagcgcgtg ccggccaggg
      661 tctccctcag gtgtcacact cgcgaatgca ggtcaggacg tggtggactt tgggctcgat
      721 cacccgatac agcacgtgca cgccgtcgcg gcgggcggcg accacgttgg cgccgcgcag
      781 gcgcgccagg tgctgcgata cgatcgcctg cggcaaccgc acggcgtccc ggatctcgga
      841 gacgctcgct tctccccgct ccagcacttc cacgatcttc aggcgctcgg gatgtccgag
      901 cagcttgatg atgtcggccg cgcgcgccag gatggcgggg tcgatgcgtc gcaacagctg
      961 cttggcggcc atgacaaata aactaatagc tatacttcta tgtagtcaac cccactgctc
     1021 ttcccccacc agccgctcgg cgcctcaacg tctggtcgcc gccggccaga ggtccgacca
     1081 ctcggatggc agcacgctgc gcacatccga gatctcgccg ccggacacgt gctcgtccag
     1141 cagccggaat accgcgcggg tgacgtcctc cggatcggcc tccggaatgc cccggagcag
     1201 cggcccgcgc accatgtcca ggaactcctc cctggaacgc atccgctgcg gcgtggcact
     1261 cgggcgccag ccgtcgaagt acaccccctt gatgagcagc gggagctgcg ccgccagctg
     1321 gaccgcctcg ttggccgtca gccggtcccg cagcgcgtgc aacaccgccc gcagcgcgag
     1381 cagggtggcg tccaggtcac cccatcccag ctggccgtcg agctcctgca gccacgcatt
     1441 cgccttgtgg accgcccggt cccacacatg cgacccgccc gccatggccg gctcctttcg
     1501 aaacggttac gccccgaccc ggggactctg cttctcgagc agccggccga tcggccccgc
     1561 ctcttcgatc agcagtccca ggatgtcgtc gatgctgagt atcccgacca gccgtttccc
     1621 ttcacccgtc acgatgagcc gccgcgtgcc cttctcggcc atgcgggtga tcgcttcctc
     1681 gatggacgct tcttcgtcga tcgccttcac cggccgcgtc atgatctgtg caacggtggc
     1741 gcgatccggg tccagaccac ccgccacgca gcggagcgcg atatcccggt ccgtcaccat
     1801 cccgaccgcc tcacccgctc cgtcgccctc cacgaccacg agggtcccga cttcctgctc
     1861 cgccatccgg cgcgcggcca cccgcaccgg ttcattgggc catgccgtag caaccatccg
     1921 gctgcaaatc cttcccgcag acatagatcc tcctttggga ccgtaccgac agttctctgg
     1981 ctgcagctta ggtcgaagag gacatggtgg gcgtgaaacg ggcctgagat acccttccgg
     2041 cgggtggcgg cggactgaac gatccttcgg attgtcctca tgacctcctc gtcgggcgac
     2101 ccttagcgtc ggacaggaga agaaccatga ccgctctccc gacacggccc ctgggcgaga
     2161 ccgggctgca catcacaccc atcggcatgg gcacatgggc acttggcggc ccgggctggg
     2221 cgttgagctg gggtctccag acggatgcgg cgtcgctcgg cgcgatgcat cacgcgctgc
     2281 ggcgggggat caactggatc gacaccgcgc cggtctacgg gttggggcac gccgaggagc
     2341 tggtgggacg cctcctcaag ggtctgccgg cggccgagcg gcctttcgtg ttcaccaagt
     2401 gcgggatgca gtgcgatcca cgagacccga gtcgcgagcc gcgccgcgac ctgcggcccg
     2461 cgaccatccg tcgtgagtgc gaggcatcgc tgcgccggct gggggtcaag cagatcgacc
     2521 tctatcagat ccactggccc gacgagttcg ggctccccgt cgagtactcg tgggcggaaa
     2581 tgatccgcct gcgggacgaa gggaaggtcc gcgcgttggg cgtgtgcaac ttcgacgcct
     2641 ggctattgac gaactgcgcc gagcgctgcc cggtagactc gctgcaagcg ccgttctcgc
     2701 tgatccggcg cgcggccgcg ctgaccgaca tcccctggtg cgtcgaccac ggcaccggcg
     2761 tcctggccta cagcccgctc gcgtccgggc tcttgagcgg cgccttcacg gaggcccgcc
     2821 tgcagcgcct ggccgcggac gactggcgcc ggaccgcacc ggagttccgc cagccgcggc
     2881 tgggccacaa tctggcgctc gcgcgggccc tgcgaccgat cgcgcaccgc cacggtacga
     2941 gcgtcgccgc ggtcgcggtg gcgtgggccg cgcactggcc cggcgtcacc ggcgcgatcg
     3001 tcggggcacg cgccccgcac caggtggacg actggatcgg cggcgcgacg ctggagctgg
     3061 tcgccgagga tctgaccgag atgaccgcag cgatcaaaca cagcggcgcc ggcgacggac
     3121 cggcgtcgcc gcaggacgtg gaagcatggc agtgggtcgg cgttcaggag tgaccggtat
     3181 ctcccccagt acacccggcg cgtagcgggt gtgaaaggat cttccgtgac tgccttttct
     3241 cacctcgccg ccaccagccc ggcgcctcgc cgattcaatg tcgtgttgcc ggcggagacg
     3301 ttcagcgagc tgaatcagct cgcgagccgg tgcggccgga gtccggccga ggtcgtccag
     3361 acggcgctcg acctgctcaa ggtcgccgac gacgaggcgt ttcgcggcaa cgtgctcgcg
     3421 atcgcgaccc gcgacggccg gccattgcgc cagatcgtca ttccgcgctg attccccggc
     3481 gtacacgcag gacggccccg gcgtccagct gaccggggcc gtcgacatcc ctgtttgtca
     3541 gtcgccgcag cgaactgtca acgccgagaa cgatgcattc ccaggaccga ccgaaacgcg
     3601 ggtgacgata ctcccacgga acggccccgc cgatggaatc tcgatctgca tgttgaagct
     3661 cgccgccagc gtcacgttgt ccgtgaggat cgcgtggtgc tgttcgttga tcagcgcctg
     3721 atagggctgg cccaccggct cgccggtgct cccgtcgatc agggtcagat cgagatgtcc
     3781 ccgcaactgg aacgtgctga ggtaattgcc cgaaggcgtg agcacgacat gctgcgtgaa
     3841 ggtcagggga ccctggacga gcagcagatc gaacggagtc tcccccgtgc agatcggctt
     3901 cgggatggtc tgatcgaact cgacttcgaa gcgctgctgc gccacgagcg gctcaccatt
     3961 gacgccgtcg tcgagggtca tcatggtggc cgtgccgtcg ttcgggaccg ggaagtccgc
     4021 actctgctcc gacagcgggt attgcagcag cgcgaggagc tggaacccat tcggatcggc
     4081 cagcaagccg ttgtagatca ctgccgggat ctcacggaac ggcggaggag tgttgatggc
     4141 cccggtctgc gagacggcgc ccagcaaccc tgttcgcgag aagtcgaacg ccacgatggc
     4201 ttccgcaaac tcccagctca gaacctgatg ctcgggggtg ccggggatct gcacctcaaa
     4261 atgtgcgccg cccacggtcc cgccgccgag gtcgaacaga cgcatatgaa agcggacggc
     4321 gtactcgcca cacgcgagct ggatcacgcc ccccgtccac ccggtggccg atccgaacgt
     4381 cgtctgcact gtcccctccg gtacatcgtg ccacgtgcag ttgaaggggg cgacatcagg
     4441 aaggccaatt cccgagcgat ccccgtcgag caggagcagc gcggcccgca gggcgcgcgg
     4501 gtcggcctgc ccgaagagca cgaggttgat gggatcttct tgcgtgccgg agaagtcgtt
     4561 ccccgtccag ggccagaact cgagtgaact gtcgccgttc gtgaccgtta cgatgggtgt
     4621 aggagtgacc tgctccttgg agatgctggc gaccacccgc gactccgtgg ggccggagcg
     4681 ctcggcacac ccccccaagg caacgacgag cgatgcggca gccgccgcgt acgcggcggt
     4741 gcgcagatga cgcgtggaca tggggacacc tccgatcggt aaggcgtccc gcgacgggct
     4801 cggcgtggag agtggcaggc gtagcggcgg ggcgccgcag ccccaagctg acgcacatcc
     4861 cttgaaacga gcttgaaacc ccgatgaaaa cggcttagct aggagcgatg aaaacccctg
     4921 ccgtcctgct cgccgcgctg gccggcgcgg gttgcgcctc gcaccgcgta ctcgtcccgc
     4981 cgcgcctcga tctggctccg tacaacagca tcggcctcgt cacgttcacg gtcgagaacg
     5041 ccaggggcgg cctcggcgac ctcgccacgc ggcgtttcga ggagtacatg ctcggcgcgc
     5101 agaaggggtt cgaggtgcag ggttttgccg ccgcggactc ggccgccgca ctctccggtg
     5161 cgcggggcgt cccggtggtt ttcctgggcc gtctcaaggt gtcgaacgtc aagccaagcg
     5221 gcgccctcgg cttggtgtcg gctcacctgg aagcgaccgt gtcggcggag ctttcggtcg
     5281 aactgcgctc caccaggacc ggcggcaccc tgtggcgcgc cagctccacc gcgagggaga
     5341 aggtaggaca ggtcgccgtg cagggtggtc ttcccgattt ctccgcccgc gatcccaatg
     5401 atgcgtatgg ccgcctcgtc gatcggctgg tgcacgcggt gacttgggac ctgcgctcga
     5461 cctgggtgaa gcagtaggga cgtcggcttg aagcccgttt catcttcagt ttaggcccgg
     5521 tt
//
LOCUS       DBNGZW010000415         2110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_924823, whole genome shotgun sequence.
ACCESSION   DBNGZW010000415 DBNGZW010000000
VERSION     DBNGZW010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_924823"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(71..622)
                     /locus_tag="AC16JC_09790"
     CDS             complement(71..622)
                     /locus_tag="AC16JC_09790"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5165024.1"
                     /translation="MATADPAVRPADDAELIAAWLAGDESAASELVRRHARALARFLA
                     GAGAQEADVDDLVQESFLRAFRALDRFRHQCQFRTWLLTIGGNVLKDLGRRLARRRIV
                     PLDEAVESRDGDPHQRAEAGETEERLRRGLERLPRMQREVFLLRAQQGFEYEDIAAAL
                     DTTPGAARVHYHHAVKRLKEWVK"
     gene            708..1301
                     /locus_tag="AC16JC_09795"
     CDS             708..1301
                     /locus_tag="AC16JC_09795"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5165025.1"
                     /translation="MRPYDERFDALLGHAARVFARRGYDRTTMRDLAAASGMSLAGIY
                     YYVRGKEDLLAHIQERCFSQVLEGAEAALAGAVDPLDRLAAFIRHHVTFFAAHMPEMK
                     VLSHEAAALTAERQRTINLIKRRYVDRLEGLLRDAAPDQIPAERSAAAYALFGMMNWI
                     YNWYDPAGPLDAGRLADLLTRLFLGGFAEVRSPVQGG"
     gene            1335..>2110
                     /locus_tag="AC16JC_09800"
     CDS             1335..>2110
                     /locus_tag="AC16JC_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006976591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5165026.1"
                     /translation="MKTLVRYHVEDGLAIIELDDPPANTYTHEMMRQLDDAILQARFD
                     DNVHVIVLRGHGDKFFSAGANISYLNSITPRYKYFFCLHANETLNRLEHTPKLTIAAL
                     NGHTVGGGLEIAMACDIRLARENAGKIGLPEVSLGVLPGTGGTQRLARLVGKSKAIEM
                     MVKGETFQMDSAREMGLVNEVFPSKDFWNMVLLYAKQFCPPNKASKAVGLIKRSVQSG
                     AEVPFESGLAIERELQQQLFQSEDAKEGIAAYTEKRMAEF"
BASE COUNT          357 a          747 c          671 g          335 t
ORIGIN      
        1 cgctctgcgg cccgcgttcc caaccgtcgg gcgagccgct ccagctcgac gtcgcgatct
       61 tcgtccggag tcatttcacc cactccttca accgtttcac cgcgtgatgg tagtggaccc
      121 gcgccgctcc cggcgtcgtg tccaatgcgg cggcgatgtc ctcgtactcg aacccctgct
      181 gggcccgcag cagaaacacc tcccgctgca tgcgcggcag ccgctcgagc ccgcggcgca
      241 gccgctcctc cgtttcgccg gcttccgcgc gctggtgcgg gtccccgtcg cgcgattcca
      301 ccgcttcgtc cagcgggacg atccgccgcc gcgccagccg gcgtcccaga tccttcagca
      361 cgttgccgcc gatcgtcagc agccaggtac ggaactggca ctggtggcgg aagcgatcca
      421 gcgcccggaa ggcccgcagg aaggactcct gcaccaggtc gtcgacgtcg gcttcctggg
      481 ccccggcccc cgccaggaac cgcgccagtg cccgggcgtg acgccgcacc agttcgctcg
      541 ccgcggattc gtctccggcg agccaggcgg cgatcaactc ggcgtcatcc gccggccgga
      601 cggccggatc cgcggtggcc atgagtgcaa ggttgacgtg ggagaaggct ggaaattaag
      661 tccctggggc actaccttca caattcgaac aaacgttcga aaacctcttg cgcccctacg
      721 acgaacgatt cgacgccctg ctgggccacg ccgcccgcgt tttcgcccgc cgcggctacg
      781 atcgcacgac catgcgtgat ctggcggcgg cgagcggcat gtcgctggcc ggcatctact
      841 attacgtgcg cggtaaggag gacctgctcg cgcacatcca ggagcggtgc ttcagccagg
      901 tcctggaggg cgcagaggcc gcgctggcgg gcgccgtgga ccccctggat cgcctcgccg
      961 cgttcatccg gcaccacgtc acgttcttcg ccgctcacat gccggagatg aaggtgctgt
     1021 cgcacgaagc cgcggcactg acggccgagc ggcagcgcac catcaacctg atcaagcgcc
     1081 ggtatgttga ccggctcgag gggttgctgc gcgatgcggc gccggaccag attcccgccg
     1141 agcgctcggc ggcggcctac gcgctgttcg ggatgatgaa ctggatctac aactggtacg
     1201 accccgcggg accgctcgac gccggccggc tcgcggatct cttgacccga ctcttcctcg
     1261 gcggattcgc cgaagtccgt tccccggtac agggaggctg agcagcgatc atggcgacta
     1321 tgacggaacc ccaggtgaag accctcgtgc gctaccacgt cgaggacggc ctcgcgatca
     1381 tcgagctgga tgatccgccg gccaacacct acacccatga gatgatgcgg cagctcgacg
     1441 acgccatcct gcaagcgcgc ttcgacgaca acgtgcacgt catcgtgctg cggggccacg
     1501 gggacaagtt cttctccgcc ggcgccaaca tcagttacct gaacagcatc accccgcgct
     1561 acaagtattt cttctgcctc cacgccaacg aaaccctgaa ccggctcgag cacacgccca
     1621 agctcacgat cgccgcgctc aacggccata cggtgggcgg cggcctcgag atcgccatgg
     1681 cgtgcgacat ccgcctcgcc cgcgagaacg ccggcaagat cggattgccg gaggtcagcc
     1741 tcggcgtgct tccgggcacc ggcggcacgc agcgcctcgc tcgcctcgtg ggcaagtcca
     1801 aggcgatcga gatgatggtg aagggtgaga cgttccagat ggactcggcc cgggagatgg
     1861 gcctggtcaa cgaggtgttc ccctcgaagg atttctggaa catggtcctg ctctacgcca
     1921 agcagttctg cccgcccaac aaggcatcca aggcggtggg cctgatcaag cgctcggtgc
     1981 agtcgggcgc cgaggtcccc ttcgagtccg gcctcgccat cgagcgggag ctgcagcagc
     2041 agctgttcca gagcgaggac gccaaggagg gcatcgccgc ctacaccgag aagcggatgg
     2101 cggagttcaa
//
LOCUS       DBNGZW010000416         2721 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_856625, whole genome shotgun sequence.
ACCESSION   DBNGZW010000416 DBNGZW010000000
VERSION     DBNGZW010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_856625"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1463
                     /locus_tag="AC16JC_09805"
     CDS             <1..1463
                     /locus_tag="AC16JC_09805"
                     /inference="COORDINATES: protein motif:HMM:NF014427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5165027.1"
                     /translation="QSVAQVSLTGIVKAVGSGKATLTVSGFLQQKTVEVLVHRAVEFL
                     AVRPTTREDVEVPLTATVRFIAAALAADSSPVPEALLGWSVADTAIAAFDPATHTLTG
                     KKIGRTQLTLHGPGPNPPRAVWAVSVIAGSVKLSAARLGIALNSRANLKASFMDDTGA
                     VLGPASGLTWSSDAPGVAAVSEDGTVAGSSYGRARITATAPGGKSATADVFVEDELLL
                     VSTRSGTSRLYWLDRSNLAALRRVTQDTAGATDPAVSPDGSRIAFVSMRDGNPEIYVM
                     DADGTNLTRVTSDPQADGRPVFTADGAGIVFQSLRSGKPQIWTAAIDGSGVRALTTDS
                     VNQTPAVSPDGGTIAYASVRNKNYDIWLMSRDGTNQRAFTKAAQQNETEPRFLRDGSL
                     AYIVERREANRTVRMVVKADLATGAITPLTGTDLPIATFAVSSRGDLIALVVPTDPNN
                     RRNPLYRIQLQTVGAGAPTPVPAGPTEQMMAPTFLP"
     gene            1493..2506
                     /locus_tag="AC16JC_09810"
     CDS             1493..2506
                     /locus_tag="AC16JC_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR5165028.1"
                     /translation="MEHTAVANVPDAPDPGPGEVRLAVQAAALNHLDLFVVEGVPGIG
                     YRFPHILGADGAGIVVDVGSGVSSVRPGDRVLVNPGISDYTCEFCRAGEHSLCVQYGI
                     LGEHHSGTLAERLTVPVHNVLPLPSLDPPLTWCEAAAFSLVTLTAWRMIMTRAQVKAG
                     EWVLAWGIGGGVSLTAMRIAKLAGARLIVTSSSDEKLERARAMGADVTLNHRTQRVVT
                     EVRALTGKRGVDVVVENVGEVTWGDSLRCLAKGGRIVTCGGTTGPKLVTDVRPLFWHQ
                     FSILGSTMGNAAEYREIVRRLGEGQLRPVMDAEFPLERTREAFERLARGEQMGKIAVT
                     IAG"
BASE COUNT          424 a          949 c          952 g          396 t
ORIGIN      
        1 atcagagcgt agcccaggtc agcctgacgg ggatcgtgaa agccgtcgga tcgggaaagg
       61 cgacgctcac cgtgtcgggc ttcctgcagc agaagacggt ggaagtgctg gtgcaccgcg
      121 cggtggagtt cctggcggtg cggccgacca cccgggagga cgtcgaggtg ccgctcaccg
      181 caaccgtgcg cttcattgcc gcggccctcg cggccgacag ctcgccggtg cccgaggcgt
      241 tgctggggtg gagcgtggcg gacaccgcga tcgccgcctt cgatccggcg acccacaccc
      301 tgacgggaaa gaagatcgga cggacgcagc tcaccctgca cgggcccggt cccaacccgc
      361 cgcgggccgt ctgggcggtg agcgtgatcg cgggctccgt gaagctctcg gcggcccgcc
      421 tcggcatcgc gctcaactcg cgggcgaacc tcaaggcgag cttcatggac gacacgggag
      481 cggtgctggg ccccgcgagc gggctcacct ggagctccga tgcgccgggc gtcgccgcgg
      541 tctccgagga cggcacggtc gcggggtcga gctacgggcg cgcccgcatc acggcgaccg
      601 cccccggagg aaaatccgcc accgccgacg tcttcgtcga ggacgagctg ctgctggtca
      661 gcacgcgcag cgggacgtcg cggctctatt ggctcgaccg ctcgaacctc gccgcgctgc
      721 gccgggtcac acaggacacc gcgggcgcga ccgatcccgc ggtctcgccg gacggctccc
      781 ggatcgcctt cgtgtccatg cgcgacggca atccggagat ctacgtgatg gatgcggatg
      841 ggaccaacct gacccgcgtc accagcgatc cccaggcgga cgggcggccg gtattcaccg
      901 ccgacggtgc cggcatcgtg ttccagtcgc tgcgctccgg gaagccccag atctggacgg
      961 ccgccatcga cggcagcggc gtcagggccc tcaccaccga ctccgtgaac cagacgcccg
     1021 cggtctcccc cgacggcggg accatcgcct acgcctcggt ccgcaacaag aattacgaca
     1081 tctggctgat gagccgcgac ggcaccaatc agcgggcctt cacgaaagcg gcgcagcaga
     1141 atgagaccga gccgcggttc ctgcgggacg gcagcctggc ctacatcgtg gagcgccgcg
     1201 aggcgaaccg cacagtgcgc atggtggtga aggcggacct cgccacgggg gcgatcacgc
     1261 cgctcaccgg caccgacctg cccatcgcga ccttcgccgt ctcgagccgc ggcgatctca
     1321 tcgcgctcgt ggtgccgacc gatcccaaca accgccgcaa tccgctctac cgcatccagc
     1381 tgcagacggt gggtgccggc gcaccgacgc ccgtcccggc ggggccgacc gagcagatga
     1441 tggcgccgac gttcctgcca taagagcgtt cgtcctccac gccaccggcg ggttggagca
     1501 caccgccgtc gcgaatgtcc ccgacgcgcc ggaccccggc cccggcgaag tccgtctcgc
     1561 cgtccaggcc gcggcgctga accacctcga cctgttcgtc gtggaagggg ttcccggcat
     1621 cggctaccgg tttccccaca tcctcggcgc cgacggggcg gggatcgtcg tcgacgtcgg
     1681 cagcggcgtg tcgagcgtgc gcccggggga ccgcgtgctc gtgaaccccg gcatttcgga
     1741 ttacacctgc gagttctgcc gcgccggcga gcacagcctc tgcgtccagt acggcatcct
     1801 gggcgagcat cattctggaa cgctggccga gcggctcacc gtcccggtgc acaacgtgct
     1861 tcccctgccg tcgctggatc cgccgctcac ctggtgcgag gcggcggcgt tttcgctcgt
     1921 caccctcacc gcctggcgga tgatcatgac gcgcgcgcag gtcaaggcgg gggaatgggt
     1981 gctggcttgg ggaatcggcg gcggggtgtc gctcaccgcc atgcgcatcg cgaagctcgc
     2041 gggcgcccgc ttgatcgtca cgtcgtcgag cgacgagaag ctcgagcggg cccgagccat
     2101 gggggccgac gtcacgctga atcaccgcac ccaacgcgtc gtgaccgaag tgcgcgcgct
     2161 caccgggaag cggggtgtgg acgtggtggt ggaaaacgtc ggcgaagtga cctgggggga
     2221 ctcgctgcgt tgcctcgcca agggcgggcg catcgtcacc tgcggtggca cgacggggcc
     2281 gaagctcgtg accgacgtgc ggccgctctt ctggcatcag ttctcgatcc tcggctcgac
     2341 catggggaat gcggccgagt accgtgagat cgtgcgacgg ctgggggagg gtcagctgcg
     2401 ccccgtgatg gacgccgagt tcccgctcga gcggacacgg gaagcgttcg agcgattggc
     2461 gcgcggggaa cagatgggga agatcgcggt gacgatcgcg ggctagggtg ggcacgggcg
     2521 ccaccgcttc gctcagaagt cctgcaacga cgcccgtgcg gcgcccggtc atcgtgacca
     2581 gaatctcgtc ggcccccctc aacagatcat caactgcctg tggggttgag ggttagaaga
     2641 gccggtcgcc gcaacggcga tcccagtgca ttattgagcc cacatgtggc tcctcccgct
     2701 gttcctcgtg gcaaccgaca g
//
LOCUS       DBNGZW010000417         2798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_988513, whole genome shotgun sequence.
ACCESSION   DBNGZW010000417 DBNGZW010000000
VERSION     DBNGZW010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_988513"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="AC16JC_09815"
     CDS             <1..276
                     /locus_tag="AC16JC_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165029.1"
                     /translation="VHNFFYKAPPGTWPIPGMGIDELMASGALFGACNMAIKFYAGIV
                     GKQMNMKPDDVANDWRANLLPTVQVVPSGVWALNRVQELGCSYCFAG"
     gene            411..1499
                     /locus_tag="AC16JC_09820"
     CDS             411..1499
                     /locus_tag="AC16JC_09820"
                     /inference="COORDINATES: protein motif:HMM:NF025006.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HIR5165030.1"
                     /translation="MVLLLLTCGAGVLGAQSIRTTLLGAGTRQPVAGAFVVVVDTTGG
                     EIVRTLTDETGGVRLDLPPGTYRLFVLRIGMARWSGTPFTLEVGDTLSTPIEAPEMPV
                     MLSEITVHADRRCQIQPEEGSAAAALWEEARKALEATEWTITHPVYRFQAGRYVRTFG
                     PTGKRPLAEERHNLSGSLSWPYASLAPESLVAHGFAQPDSEGVTTYYGPDLPVLLSGV
                     FLERHCFRLARASGTAADSLLGLAFEPVGGRRAVDVSGVLWLERRSAALDALEFRYTN
                     LGPWAGRGAGGRVAFQRLPNGAWVIRRWHIRMPIPLIGRRLRSAPGELELGSPDTVGV
                     AGYREEGGWIALILSADGRIVATYPEEP"
     gene            complement(1513..2247)
                     /locus_tag="AC16JC_09825"
     CDS             complement(1513..2247)
                     /locus_tag="AC16JC_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165031.1"
                     /translation="MHLAILLLTGLLAGREPQTGDALVRAMHDRYDGQWYRTLAFVQH
                     NTLYLPGDSVQHSTWFERAAIPGTLRIDFRDGPGFPPDGGVLFARDTQFVIQGDSLVR
                     ADAFVHPLMVLGFDVYAQPVEQTLGKLRQLEFDLATTHEDTWQGRPVVVVGAKAGDLH
                     TRQFWIDKERLVFVRMLEPARRDSTVTTETQFNDYRPFGKAWVSAQVLFLTGGQRRWL
                     EEYDRIAVNVALADGVFDVKRWKDTK"
     gene            complement(2277..>2798)
                     /locus_tag="AC16JC_09830"
     CDS             complement(2277..>2798)
                     /locus_tag="AC16JC_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR5165032.1"
                     /translation="MDGRDVCRELRQRANTPIIMLTARGEEFDRVLGLELGADDYVTK
                     PFGVLELLARVEALMRRAVATVATSEAAAPLVEKFGDVEIQCASRTVLRKGDAVPLTP
                     KEYELLLALIRRRGAVASRVELLTEVWGYSAAVLSRTVDTHVAELRRKLEDDLQAPRH
                     ILTVRKAGYRLQA"
BASE COUNT          406 a          936 c          998 g          458 t
ORIGIN      
        1 gtgcacaact tcttctacaa ggcgccgccc ggcacctggc cgatcccggg gatgggcatc
       61 gatgagttga tggcgagcgg cgcgctgttc ggcgcgtgca acatggcgat caagttctac
      121 gccggcatcg tcggcaagca gatgaacatg aagcccgacg acgtcgccaa cgactggcgg
      181 gcgaacctgc tgcccaccgt gcaggtcgtg ccctccggcg tgtgggcgct gaaccgcgtg
      241 caggagctgg gctgcagcta ctgcttcgcc ggttgacctc gtagtgcgga tgcggtcggg
      301 gctcgcgtcc ggcgacgcgg gccccgatcg cattgcttca gctccggtct cggggcaagt
      361 tctctcgcat ggaggtgtcg gggcccttcg cgggcggccg tggcgctgct gtggtactgc
      421 tgctcttgac gtgcggagcc ggcgtgctcg gcgcccagtc gatccgcacg acgctgctgg
      481 gggcgggcac gcgccaaccg gtggccggtg cgttcgtggt cgtggtggac acgaccggcg
      541 gcgagatcgt ccgtaccctc accgacgaaa ccggcggcgt gcgtctggac ctcccgccgg
      601 gcacctaccg cctgttcgtc cttcggatcg gcatggctcg ctggagcggc acgccgttca
      661 ccctggaggt gggcgacacg ctctcgacgc cgatcgaggc gccggagatg ccggtgatgc
      721 tgtcggagat caccgtgcac gccgatcggc gttgtcaaat tcagcctgag gaagggtcgg
      781 ccgcagccgc gctgtgggaa gaagcccgca aggcgctgga agcgaccgaa tggacgatca
      841 cgcatccggt gtaccggttc caggctggcc gctacgtccg cacgttcggc ccgacgggaa
      901 aacggccgct cgccgaggag cgccacaatc tctccggatc attgagctgg ccgtatgcca
      961 gcctcgcgcc cgagagcctg gtcgcccacg ggttcgccca accggactcg gagggcgtga
     1021 cgacgtatta cggacccgac ctgccggtgc tgctgtccgg ggtgttcctg gagcggcact
     1081 gtttccgcct cgcgcgcgcg agcggtaccg ctgccgattc cctcctcgga ttggccttcg
     1141 agccggtcgg gggtcgtcga gccgtggatg tcagtggagt cctgtggctc gagcgccgtt
     1201 cggcggcgct cgacgcgctc gagtttcgct acaccaacct tggcccctgg gcgggccgcg
     1261 gggcgggtgg acgggtggcg ttccagcgcc tacccaacgg cgcctgggtg atccgccgct
     1321 ggcacatccg gatgcccatc ccgctgatcg ggaggcggct gcggtcggcg ccaggggagc
     1381 tggaactggg atcgcccgac acggtggggg tcgccggcta tcgggaggaa ggagggtgga
     1441 tcgcgctgat cctgagcgcc gacggccgga tcgtggcgac ctatcctgaa gaaccttgat
     1501 cctggatcgt cgctacttcg tgtccttcca gcgtttcacg tcgaacacgc cgtcagcgag
     1561 cgcgacgttc acggcgatcc ggtcgtactc ctccagccag cggcgctggc cgccggtcag
     1621 gaacaacacc tgcgccgaca cccaggcctt gccgaacggc cgatagtcgt tgaactgggt
     1681 ttcggtcgtg acggtcgagt cgcgccgcgc gggctccagc atgcgcacga agaccagacg
     1741 ctccttgtcg atccagaact gacgggtgtg caggtcgccg gccttcgcgc ccaccacgac
     1801 caccggccgt ccctgccagg tgtcctcgtg cgtggtggcg agatcgaact ccagctgccg
     1861 cagcttgccc agggtctgct cgacgggctg ggcgtatacg tcgaagccga gcaccatcag
     1921 cggatgcacg aacgcgtcgg cccgcacgag cgagtcaccc tggatgacga actgcgtgtc
     1981 ccgggcgaag aggacgccgc cgtccggcgg gaatcccggt ccgtcgcgga aatcgatgcg
     2041 cagcgttccc gggatggcgg cgcgctcgaa ccaggtcgag tgctgcacgc tgtcgccggg
     2101 gagatagagc gtattgtgct gaacgaacgc gagcgtacgg taccactggc cgtcgtaccg
     2161 gtcgtgcatc gcgcggacga gcgcgtctcc ggtctgaggc tcgcgtccgg cgagcaggcc
     2221 ggtcagcagc aggatggcga ggtgcacgtg cggtctccgg atcggtggta gccgggtcag
     2281 gcctgcagtc ggtagcccgc cttgcgcacg gtgaggatgt ggcgcggcgc ctggaggtcg
     2341 tcctccagct tgcgacgcag ctccgccacg tgcgtgtcca cggtgcgcga cagcacggcc
     2401 gcgctgtacc cccagacctc ggtcagcagc tcgacgcgac tcgccacggc tccgcggcgg
     2461 cggatgaggg cgagcagcag ctcgtattcc tttggcgtga gcggcacggc gtctcctttg
     2521 cggagcaccg tgcgggaggc gcactggatc tccacgtcgc cgaacttctc gacgagcggg
     2581 gccgcggcct ccgaggtggc gaccgtcgcg actgcgcgcc gcatcagcgc ctccacccgc
     2641 gcgagcagct cgagtacgcc gaacggcttg gtgacgtagt cgtcggcgcc gagctcgaga
     2701 ccgagcacgc gatcgaactc ctcgccacgc gccgtgagca tgatgatcgg cgtgttcgcc
     2761 cgctggcgga gctcccggca cacgtcgcga ccgtccat
//
LOCUS       DBNGZW010000418         5797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_311544, whole genome shotgun sequence.
ACCESSION   DBNGZW010000418 DBNGZW010000000
VERSION     DBNGZW010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5797
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_311544"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..1285
                     /locus_tag="AC16JC_09835"
     CDS             68..1285
                     /locus_tag="AC16JC_09835"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018717882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5165033.1"
                     /translation="MPFASLGLVPELVRAVFEEGYAHPTPIQQETIPLALAGRDVIGS
                     AQTGTGKTAAFLLPILQRLNREKRPRTLRALILTPTRELAEQVLQSARTYGRHLPLES
                     VAVYGGVGMEPQTRALTRGVDIVVATPGRLLDHMERGHVDYSRIEVLVLDEADRMLDM
                     GFAPDVRRILRALPDERQTLLFSATISPEVDALARSALSGHASVDIGRRAQPAEGIEH
                     VIVAADKLKKRDVLAAILKAKPAGQTLIFTRTKYGADKLQAFLRKSGIGANAIHGDKA
                     QSHRTRTLDAFRAGSAEVLVATDIAARGIDVDGIRMVVNFDVPNDPETYVHRVGRTAR
                     AGARGLALTLLSPDEWLLMGDIEKLVGQVFPREIIPGFEPSVAPLQPKHAESAVKRPS
                     LTVRGRAGGRRRR"
     gene            complement(1441..2727)
                     /locus_tag="AC16JC_09840"
     CDS             complement(1441..2727)
                     /locus_tag="AC16JC_09840"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR5165034.1"
                     /translation="MPTDSLTIVDNRTGRTYEIPVQEGTIRAADLRQIKTGPDDFGLM
                     SYDPAYLNTAACRSAISFIDGDKGILRYRGYPIEELAERSSFLETAYLLLNGELPRPA
                     ELDRWVYDITHHTIIHENVKKFIDGFHHDAHPMGMFVSTVAALSTFYPEAKHIGDGEA
                     RRFQIHRLIAKTPTLAAFAYRHSTGMPFAYPDNDLSYAGNFLNMMFKGTEVKYKPHPV
                     AERALDVLFILHADHEQNCSTSAMRGVGSSHSDPYSSMAAAAAALYGPLHGGANEEVL
                     RMLREIGSKDRVPSYIERVKRGEVKLMGFGHRIYKNYDPRARIIKRVADEVFEVTGKN
                     PLIDLALELERIALQDNYFVTRKLYPNVDFYSGIIYEAMRFPVDMFPVLFAIGRTPGW
                     LAQWQEMLLDKEQKIARPRQLYVGPPMRAYVPIEKR"
     gene            complement(2836..5469)
                     /locus_tag="AC16JC_09845"
     CDS             complement(2836..5469)
                     /locus_tag="AC16JC_09845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165035.1"
                     /translation="MNRTLWPILAGALVLAPTALPGQRRTAPAPAPAARLLDTLLYRS
                     LKFRAIGPSTMGGRMSAFAVREANPRVIFAAAGTGGLFKTTNNGTTWSAVFEDQPVAS
                     IGAVAVSQRDSNLVWVGTGEGNGRNSSSWGNGVYRSTDGGGKWTHLGLDNTHDIPALV
                     IDPRNDSTVWVAALGHLWGKNPERGVYKTSDGGKTWTAVLQLGDSVGAIDLVLDPNTP
                     DVAYAAMYQRLRAPWAMRSGGPVGGIYKTIDGGRTWAKLAGGLPPQTGRIGLDIYRKN
                     PQIVYAVIESDAGGPKDIDDVHSRAGGIFRSDDGGAHWRRLNDLAPRGFYFAKIRVDP
                     TNDAHVYVLGYGLDVSDDSGHTFRHDGAEKIHGDLHALWIDPGNPDHLILGTDGGIYF
                     SWDRSKTWDFINNVATGEFYEVAYDYRTPYRICGGLQDNGSWCGPSRKRAEDGIAASD
                     WVRVGDCDGYYVQVDSTDPDIVYAECQQGYAFRVNQRSGAVKSIRPNPKEGTPAFRFN
                     WNTPFAISHFDPTVLYFGGNQLFRLSARGDAWETISPDLSTMNPQTMTAAGSGAETHN
                     TIVTLSESPLDPNVIWAGTDDGNIQLTRDGGATWTSVGANLRGLPKPNLYVARIEASH
                     FDSGTAYVAIDGHRSDVFQPLAFMTTDYGRTWTSIAGTLPAGGPLKALREDPRNRNLL
                     FAGTEFGLYFSLDRGAAWNRFRNGLPTVAFDDIAIHPRDRDLIVATHGRSFYILDQVI
                     PLEELTPAVLDSAAFLFSTRPVQPFWKLQESGLWGHRPYQGANPPVGAPIDYYLKTYA
                     LDDVSITIADAGGREVAKLTGSKDPGLNRVTWDLQPKESLRAGSPGDPTEYVAAGTYT
                     VTLSASGRTLKTTLQVLEPPK"
     gene            5525..>5797
                     /locus_tag="AC16JC_09850"
     CDS             5525..>5797
                     /locus_tag="AC16JC_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165036.1"
                     /translation="MTSPAAPRPSLYPTRSAAGADLGKVLASQGYVHCLLLGVTPEGV
                     EIAANAAKAMGASFDVLVAAFIRLGDNLAPVGALAENTTSAEMDPDF"
BASE COUNT          894 a         1884 c         2029 g          990 t
ORIGIN      
        1 actgagagtg gcgacattcc gtctccacga gtgcgcgtgt gtgctgtaaa ggaccatgga
       61 ccacaacatg ccatttgcct ccctcgggct ggtgcccgaa ctggtgcgtg ccgtattcga
      121 agagggatac gcgcacccca cgcccattca acaggaaacc attccgctgg ccctcgcggg
      181 ccgcgacgtc atcggctcgg cgcagaccgg caccgggaag acggccgcgt tcctgctgcc
      241 gatcctccag cgcctcaatc gcgagaagcg gccgcgcacg ctgcgggcgc tgattctcac
      301 gcccacccgt gagctcgccg agcaggtgct gcagagcgcg cgcacctacg gcaggcacct
      361 gccgctcgag agtgtcgcgg tgtacggcgg cgtcggcatg gagccgcaga cccgggccct
      421 cacccgtggg gtggatatcg tcgtcgccac gccgggccgc ctgctcgatc acatggagcg
      481 cggccacgtc gactattcca ggatcgaagt gctggtgctg gacgaggccg atcgcatgct
      541 cgacatgggg ttcgcccccg acgtgcggcg tatcctccgg gcgctgcccg acgagcgcca
      601 gacgctgctc ttctcggcga cgatctcgcc ggaggtcgat gcgctggccc gcagtgcgct
      661 gtccggccac gcttcggtcg acatcgggcg gcgcgcccag cccgccgagg gaatcgagca
      721 cgtcatcgtc gcggcggaca agctcaagaa gcgcgacgtg ctggcggcga tcctgaaggc
      781 caagcccgcc ggccagacgc tcatcttcac ccgcaccaag tacggcgccg acaagctgca
      841 ggcgtttctg cgcaagtccg ggatcggcgc gaacgcgatc cacggtgaca aggcgcagag
      901 tcaccgcacc cgtacgctcg atgcgttccg cgccggatcc gcggaggtgc tcgtcgccac
      961 cgatatcgcc gcgcgcggaa tcgacgtgga cggcattcgg atggtcgtga acttcgacgt
     1021 ccccaacgac ccggagacct acgtgcaccg ggtaggccgc accgcccggg ccggtgcccg
     1081 gggcctcgcc ctcacgctgc tgtcgcccga cgagtggctg ttgatggggg acatcgagaa
     1141 gctggtgggg caggtgttcc cgcgggagat cattcccggg ttcgagccgt cggtggcccc
     1201 gctgcagccg aagcacgcgg agtcggcggt gaagcggccg tccctcacgg tgcgcggacg
     1261 ggcgggcggc cggagaaggc gataagcaaa ggcgtcaagc aaaggcgtca agcagaggcg
     1321 tcaagcagag gcgtcaagca acaacgtcga gcggaaccgt acggtcctgc tcgacgtctt
     1381 tgctcgacgc tgttgctcga cgcttctgct tgacgcctta tcgtttttct gcttgacgcc
     1441 ttatcgtttt tctatcggaa cgtacgcgcg catcggaggc ccgacgtaca gctgccgcgg
     1501 ccgcgcgatc ttctgctcct tgtcgagcag catctcctgc cactgggcca gccaccccgg
     1561 cgtgcgcccg atggcgaaca gtaccgggaa catgtccacc ggaaaccgca tcgcctcgta
     1621 gatgatgccg gaatagaaat cgacattggg gtacagcttg cgggtgacga agtagttgtc
     1681 ctgcagcgcg atgcgctcga gctcgagcgc caggtcgatg agcgggttct tcccggtcac
     1741 ctcgaagacc tcgtcggcca cgcgcttgat gatacgcgcg cgcgggtcgt agttcttgta
     1801 gatgcggtgg ccgaatccca tcagcttgac ctcgccgcgc ttcacccgct cgatgtagga
     1861 cggcacgcgg tccttcgacc cgatctcccg caacatgcgc agcacttcct cgttggcgcc
     1921 gccgtgcagc ggtccgtaca gcgccgcggc ggcggccgcc atggacgaat aggggtcgga
     1981 gtgggacgag cccacgcccc gcatggcgct ggtcgagcag ttctgctcgt gatcggcgtg
     2041 caggatgaac agcacgtcga gcgcgcgctc cgccaccgga tgcggcttgt acttcacctc
     2101 cgtccccttg aacatcatgt tcaggaaatt gccggcgtag gagaggtcgt tgtcgggata
     2161 cgcgaacggc atgccggtgg agtgccggta cgcgaacgcg gccagggtcg gcgtcttcgc
     2221 gatgaggcgg tggatctgga agcgccgcgc ctccccgtcg ccgatgtgct tggcctcggg
     2281 gtagaaggtc gacagcgccg ccaccgtgct gacgaacatg cccatcgggt gcgcgtcgtg
     2341 gtggaagccg tcgatgaact tcttgacgtt ctcgtggatg atcgtgtggt gtgtgatgtc
     2401 gtacacccag cgatccagct ccgccggcct ggggagctcg ccgttgagca gcagataggc
     2461 cgtctccagg aacgagctgc gctcggcgag ctcctcgatc gggtaccccc ggtagcgcag
     2521 gatccccttg tcgccgtcga tgaagctgat ggcgctgcga cacgcggcgg tgttgaggta
     2581 ggccgggtcg tagctcatga gcccgaaatc gtccgggccg gtcttgatct gtcgcaggtc
     2641 cgccgcccgg atggtccctt cctgaaccgg gatctcgtag gtgcggccgg tgcggttgtc
     2701 gacgatggtg agggagtcgg tgggcatggg gcaaagataa agggcggacg gaggcggaaa
     2761 agggcggaag aagacgcgtc cgccctattc cgccctcttc cgccctcttc cgccttcttc
     2821 cgccttcttc cgcccctatt tcggcggttc cagcacctgc aacgtggtct tgagggtcct
     2881 cccggacgcc gacagtgtca ccgtataggt cccggccgcc acgtactcgg tcgggtcccc
     2941 gggggacccg gcgcgcagcg attccttggg ctgcaggtcc cacgtcacgc ggttcaggcc
     3001 tgggtccttg ctcccggtga gcttcgccac ctcgcgaccg ccggcatcgg cgatggtgat
     3061 ggagacgtcg tccagtgcgt aggtcttgag gtagtagtcg atgggcgccc cgaccggtgg
     3121 gttggcgccc tggtaggggc gatgccccca cagtcccgat tcctggagct tccagaacgg
     3181 ctgcaccggc cgggtgctga agaggaaggc ggccgaatcg agcaccgcgg gggtcaactc
     3241 ctccaacggg atgacctggt ccaggatgta gaagctgcgg ccgtgggtgg caacgatcag
     3301 gtcgcggtcg cgcgggtgga tcgcgatgtc gtcgaaggcc acggtgggga ggccgttccg
     3361 gaagcggttc caggcggcgc cgcggtccag cgagaagtac agtccgaact ccgtacccgc
     3421 gaacagcagg ttccggttgc gcgggtcctc gcgcagcgcc ttgagcggtc cgcccgcggg
     3481 cagggtaccg gcgatggacg tccaggtgcg gccgtagtcg gtcgtcatga acgcgagcgg
     3541 ctggaacacg tcgctgcggt ggccgtcgat cgccacgtac gcggtcccgg aatcgaagtg
     3601 cgacgcctcg atccgcgcca cgtacaggtt cggcttgggg agtccccgca gattcgcccc
     3661 gacgctggtc caggtggcgc cgccgtcgcg ggtgagctgg atgttgccgt cgtcggtccc
     3721 cgcccagatg acgttggggt cgagcggaga ctccgacagt gtgacgatgg tgttgtgggt
     3781 ctccgcgccg gagcccgccg ccgtcatcgt ctgggggttc atggtggaaa ggtcggggga
     3841 gatcgtctcc caggcgtcac cgcgggcgct gaggcggaac agctggttgc cgccgaaata
     3901 gagcaccgtg ggatcgaagt gcgagatcgc gaacggcgtg ttccagttga accggaaggc
     3961 gggcgtgccc tccttggggt tcggccggat ggacttgacg gccccgctgc gctggttcac
     4021 ccggaaggcg tatccctgct ggcattccgc gtagacgatg tccggatcgg tcgaatccac
     4081 ctggacgtag tagccgtcgc aatcgcccac gcgaacccaa tcggaggcgg cgatgccgtc
     4141 ttccgcgcgc ttgcggctgg gaccgcacca gctgccgttg tcctgcagcc ccccgcagat
     4201 gcggtacggg gtgcgatagt cgtaggcgac ctcgtagaat tcgcccgtgg ccacgttgtt
     4261 gatgaagtcc caggtcttgc tgcggtccca ggagaagtag atcccgccgt cggtgccgag
     4321 gatcaggtga tcggggttgc cgggatcgat ccacaacgcg tgcaggtcgc cgtggatctt
     4381 ctcggcgccg tcatggcgga aggtgtggcc gctgtcgtcg gacacgtcga gaccgtaccc
     4441 gaggacgtac acgtgcgcat cgttcgtcgg atcgacgcgg atcttggcga aatagaagcc
     4501 gcggggggcg aggtcgttga ggcgccgcca gtgcgcaccg ccgtcgtcgg agcggaagat
     4561 gccgcccgcg cgcgagtgca cgtcgtcgat gtccttggga ccgcccgcgt cggattcgat
     4621 caccgcgtag acgatctgcg ggttcttgcg atagatgtcg aggccgatcc gcccggtttg
     4681 cggcggtaac ccgccggcga gcttcgccca ggtccggccg ccatcgatcg tcttgtagat
     4741 gccgcccacc ggcccgccgc tgcgcatcgc ccagggggcg cgcagccgct ggtacatcgc
     4801 ggcgtaggcg acgtcggggg tgttggggtc gagcacgaga tcgatggccc cgaccgagtc
     4861 cccgagctgc agcaccgccg tccaggtctt gccgccatcg ctggtcttgt agacgccgcg
     4921 ctcgggattc ttgccccaca ggtggcccag ggcggccacc cagacggtcg agtcgttgcg
     4981 gggatcgatc acgagcgccg ggatgtcgtg ggtgttgtcg agccccaggt gcgtccactt
     5041 cccgccgccg tcggtggacc ggtagacccc gttgccccac gacgagctgt tgcgcccgtt
     5101 gccctccccg gtgccgaccc agacgagatt cgagtcgcgc tgcgacaccg cgacggcgcc
     5161 gatcgaggcg acgggctggt cctcgaatac ggccgaccac gtggttccgt tgttcgtcgt
     5221 cttgaagaga ccgccggttc cggccgcggc gaagatcacg cgtggattcg cctcgcgcac
     5281 cgcgaacgcc gacatgcggc cccccatggt cgagggccca atcgcgcgga acttcaggct
     5341 gcggtagagc agggtgtcga ggagtcgtgc ggcgggtgcc ggcgcggggg cggtgcggcg
     5401 ttggcccggg agcgccgtcg gagcgaggac gagagcaccc gccaggatcg gccagagggt
     5461 tcgattcatg gggccagaag atgcgtcctc tggtccgaac gctcaatcct cgtattttgg
     5521 caacgtgacg tctccagccg ctccccgccc cagcctctat cccacccgct ccgccgccgg
     5581 ggcggacctg ggcaaggtgc tggcgtccca gggatacgtc cactgcctgc tgctgggggt
     5641 cacccccgag ggagtggaga tcgcggccaa cgccgccaag gcgatgggcg cgagcttcga
     5701 cgtgctggtg gcggcgttca tccgtctggg cgacaacttg gcgcccgtgg gcgcgctggc
     5761 cgaaaacacg acatcggccg agatggaccc cgacttc
//
LOCUS       DBNGZW010000419         3150 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1333686, whole genome shotgun sequence.
ACCESSION   DBNGZW010000419 DBNGZW010000000
VERSION     DBNGZW010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3150
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1333686"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1370)
                     /locus_tag="AC16JC_09855"
     CDS             complement(<1..1370)
                     /locus_tag="AC16JC_09855"
                     /inference="COORDINATES: protein motif:HMM:NF022525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HIR5165037.1"
                     /translation="MLSKSRFVTGCQCHKLLWWTVHESNAPELQPDKVLLDLFDQGRQ
                     VGELARRRYPGGVLIDLPHTARTERVAATKDALAQGSPAVFEATFIAQDTFVAIDVLQ
                     QEGDGYRLIEVKSSNSQKDEHIPDVAVQAYIAAACGAEISSAEVLHLNPEFRHPDAGD
                     LFAGSDVTQAVAEYLPTVPDELARQQTMLEGDLPDVPIGLHCFEPRECPFLERCWPDS
                     PDHIRCLNGVGPKRSAAYLERGIQWIGDLPKTEKLTFTQQRQLKAMAANRLVLEPTLA
                     GELEPFVTAQRLGFLDFETIARAVPVWPGMAPWQQAPAQFSYHERQPDGTYTHAAFLA
                     EGPADARPRLVEAMVRATARADRVVTYTAFEKTRIREMQEAVPQLADELAALEAKLLD
                     LNPVIRNCVYHPAFRGSFSLKAILTPLVPDLTYDDLVIVDGRVASVEIARLLFVAGRI
                     PRHEHDR"
     gene            complement(1419..2525)
                     /locus_tag="AC16JC_09860"
     CDS             complement(1419..2525)
                     /locus_tag="AC16JC_09860"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5165038.1"
                     /translation="MSARVEYQREVLRQAGVALQGREVTLWEVTATDAVAPLASSISI
                     LPDHATRADLDATLRHWGAPVVEGSRWVGCRLENGLRWCVAPVRARPPAPPPGGVERR
                     SRERLILELAGLCLGAIEATVGPKPRLSPSEAAWEYARQPSVIAHEVGNQLTVAGGNL
                     EFGLASVRAAQSLDPELRAQLVEDLTHAAKGIEQATDYLRAIQERSGLGPGRVTRFEV
                     TAVIRSCVTLERPLARKRGVGLEWDALVETAYLTGDPHALYQVVTNLIRNAVDASEAR
                     HSAVQITLQPVDETLHLSVRDRGVGIAPEHESRIFEAGFTTKPPGSGSGMGLAVVREI
                     AEHMFGGRVQVKSAVGTGSTFTVVLPIPPQRAGE"
     gene            2721..>3150
                     /locus_tag="AC16JC_09865"
     CDS             2721..>3150
                     /locus_tag="AC16JC_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012376639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5165039.1"
                     /translation="MPPYRPNPARYDGMVYRRCGASGVLLPAVSLGLWHNFGAVDPFA
                     TARASVRRAFDLGITHFDLANNYGPPPGSAETTFGRILRRDLAAHRDELIVSTKAGYL
                     MWPGPYGEWGSRKYLLASLDQSLARMGLDYVDIFYHHRPDP"
BASE COUNT          466 a         1117 c         1082 g          485 t
ORIGIN      
        1 cggtcgtgct cgtggcgcgg gatcctgcca gccacgaaca gcagccgcgc gatctccacg
       61 ctcgccacgc ggccgtctac gatcacgagg tcgtcgtagg tgagatcggg gacgagcggg
      121 gtgagaatcg ccttgaggct gaagctcccc cggaaggcgg ggtgatagac gcaattgcga
      181 atgacgggat tgagatcgag cagcttcgcc tcgagcgctg ccagctcgtc cgccagctgc
      241 ggcaccgcct cctgcatttc ccggatgcgc gtcttctcga acgccgtgta ggtcacgacc
      301 cggtcggcgc gggcggtggc gcgcaccatc gcctccacga ggcgtgggcg cgcgtccgcg
      361 ggcccctcgg caagaaacgc ggcgtgcgtg taggtgccgt ccggctgccg ctcgtggtag
      421 ctgaactgcg cgggggcctg ttgccaggga gccatcccgg gccagacggg taccgcccgc
      481 gcgatcgtct cgaagtccag gaagcccagc cgttgcgcgg tgacgaacgg ctccagctcg
      541 cccgcgagcg tcggctcgag cacgaggcgg ttcgctgcca tcgccttgag ctggcgctgc
      601 tgggtgaagg tgagcttctc ggtcttcggc aagtcgccga tccactgaat gccgcgctcc
      661 aggtacgccg ccgagcgctt ggggcccacc ccgttcaggc agcggatgtg gtcgggggag
      721 tcgggccagc agcgctcgag gaacggacac tcgcgcggct cgaagcagtg cagcccgatg
      781 gggacgtcgg ggaggtcgcc ttcgagcatc gtctgttgcc gcgccagctc gtccggcacg
      841 gtgggcaggt actcggccac ggcctgggtc acgtcgctgc cggcgaacaa gtccccggcg
      901 tcggggtggc ggaactccgg attcaagtgc agcacctctg cgctggaaat ctcggcgccg
      961 cacgcggcgg cgatgtaggc ctgcaccgcc acgtcgggga tgtgctcatc cttctgtgag
     1021 ttggacgact tgacctcgat gagccggtag ccgtcaccct cctgctgcag cacgtcgatg
     1081 gccacgaagg tgtcctgcgc gatgaaggtg gcctcgaaga ccgccgggga gccctgggcg
     1141 agcgcgtctt tcgtggccgc gacgcgctcg gtccgcgccg tgtggggcag gtcgatcagc
     1201 acgccgcccg gatagcggcg gcgcgcaagc tcccccacct gccggccctg gtcgaagaga
     1261 tcgagaagca ccttgtcggg ctgcagctcg ggggcattgg actcgtgcac ggtccaccac
     1321 aggagcttgt gacactggca gccggtgacg aaccggctct tgctgagcat gtgtgggaat
     1381 ctgaacgaac cctgcgacac cgaacccagg cgctcgagct actcgccggc gcgctgcggg
     1441 ggaatcggca acacgacggt gaaggtgctg cccgtgccga ctgcggactt cacctgcacc
     1501 cggccaccga acatgtgctc ggcgatctcg cgcaccacgg cgagccccat ccccgagccc
     1561 gagcccggcg gcttggtggt gaagccggcc tcgaagatcc tgctctcgtg ctcgggcgcg
     1621 atccccacgc cgcgatcgcg gacgctcagg tgcagcgtct cgtccacggg ctgcagcgtg
     1681 atctggaccg cgctgtggcg tgcctcggag gcgtccaccg cgttgcggat caggttcgtg
     1741 acgacctgat agagcgcgtg cggatcaccg gtgagatagg ccgtctcgac cagggcgtcc
     1801 cattcgagcc ccaccccacg cttgcgggcg agcgggcgct cgagggtgac gcaggaacgg
     1861 atgaccgcgg tgacctcgaa gcgcgtcacc cggccgggtc ccaggccgga ccgttcctga
     1921 atggcccgca gataatcggt ggcctgctcg atccccttgg cggcgtgggt gagatcctcc
     1981 accagctgcg cccgcagctc ggggtcgagc gattgggccg cgcgcaccga ggcgaggccg
     2041 aattcgaggt tgccccccgc cacggtcagc tggttcccca cctcgtgcgc gatcacgctc
     2101 ggctgacggg cgtattccca cgccgcttcc gagggggaga ggcgcggctt gggccccacc
     2161 gtggcctcga tcgcaccgag acagagtccc gccaactcga ggatgagacg ctcgcggctg
     2221 cgccgctcga cgccgcccgg cggcggcgcg ggcggacggg cgcgaaccgg cgcgacgcac
     2281 cagcgcaacc cattctccag ccggcagccg acccaacgac tcccctcgac gacgggggcg
     2341 ccccagtggc gcagcgtcgc gtcgagatcg gcgcgcgtcg cgtgatcggg gagtatggag
     2401 atgctgctcg ccagcggggc gacggcgtcg gtggcggtga cttcccacag cgtgacctcg
     2461 cgaccctgca gggccacgcc ggcctgccgc agcacttccc gctggtactc gacccgtgcg
     2521 ctcatccgag cgccgcgctc aggcgcacgt cgtgcactcc ccggcacttc cgcaggacac
     2581 cgtgtgctcc ctttgagtgg agtaccgccg ctgggggccg gtcgggcaat tcccccccgg
     2641 tccaccgaat ggcgcggacc gctctcagcg gggagcgccg acgacgcgcg ccgcctacaa
     2701 agtaataagt ttcttcccct atgcctccct accgacccaa ccccgcccgt tatgatggaa
     2761 tggtctatcg ccgctgcggg gcgagcggcg tgctgctccc cgcggtgtcc ctcggcctgt
     2821 ggcacaactt cggcgccgtg gacccgttcg ccaccgcccg cgcctccgtc cgacgcgcct
     2881 tcgatctggg aatcacccac tttgacctcg ccaacaacta cggcccgcca ccggggtccg
     2941 ccgagacgac gttcggccgg atcctgcgac gcgacctcgc cgcccatcgc gacgagctga
     3001 tcgtctccac caaggcgggc tacctgatgt ggcccggtcc ctacggcgag tggggatcgc
     3061 gcaagtacct gctcgcgagt ctcgaccaga gcctcgcccg catgggcctc gactacgtgg
     3121 atatcttcta tcatcaccgg cccgacccag
//
LOCUS       DBNGZW010000420         5123 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_311712, whole genome shotgun sequence.
ACCESSION   DBNGZW010000420 DBNGZW010000000
VERSION     DBNGZW010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5123)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5123)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5123
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_311712"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="AC16JC_09870"
     CDS             <1..1213
                     /locus_tag="AC16JC_09870"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5165040.1"
                     /translation="RALPGGSAGDDPGLMREIDILVVGAGPAGSIAALEAAGAGLEVV
                     LLERDRAVGAPVRCAEGVGDAGLREFADPAGAPWAARKITRVIFLAPDDTEVQVAERD
                     VGWILDRTRFDAHLAAQAVAAGAELQVGAEATGMTRETDGRWTVAVRGSGGPDGYRAR
                     VVIGADGVETMVGRWAGLDTRVPARDMESCAQYVVQGIDFDPDAIYLQFGDGIAPGGY
                     AWLFPKGERVANVGLGMVALHSDGRTARQYLDAWITRRYPRGARTAYTVGGVIVHTTI
                     KQTYADGVLVAGDAAHMINPLSGGGIVNAMKAGRLAGRTAAAAICAGDTSARRLAGYH
                     RDWMRLLGDDHLKYYRLKQALEHLDDAFFNGLARTVNAIPREKRTLGRIFAHALVKHP
                     QLIPVAARFFV"
     gene            1321..2223
                     /locus_tag="AC16JC_09875"
     CDS             1321..2223
                     /locus_tag="AC16JC_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR5165041.1"
                     /translation="MLSIDLTGKRALVAGVADDAGFGFAIAKALAEAGATVCAGTWPP
                     ALNIFQNLLERGKIDDSRRMPDGSLLGFEKIYPLDAAFDTLADAPDDVRTNKRYKDVG
                     DFSVEGIRQRLVADFGERPLDIVIHSLANGPEVKKPLLETSRSGYLTAVSVSAYSLVS
                     LVARLAPHLRPGGSFLSLTYLASERVVPGYGGGMSSAKAALESDTRTLAYEAGRRYGV
                     RINTISAGPLASRAASAIGKIERMVDYYAANAPLAEPLPARDVGTVGAFLCSPLASGI
                     TGTTIYVDKGYHTMGMAVAGVEGL"
     gene            complement(2218..3042)
                     /locus_tag="AC16JC_09880"
     CDS             complement(2218..3042)
                     /locus_tag="AC16JC_09880"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR5165042.1"
                     /translation="MIRPPVLCYHRVGGPPELGITRVTPETFARHMTALAKDGWRTVT
                     LEQFIQADSPYLFPVARLSATGRNFLLTFDDGYESLAEHAYPVLADVGFTATTFLVTD
                     YVGRQNLWDVRYTRKRLTHLDWPAIEAWRARGFDFGSHSATHRRLTWMKDAALAEELG
                     RSRETLIGRFGAAAGRAVAYPFGAANRHVVEIARRAGFEIGFAGVWESGSDPLRLPRM
                     PVYMWDKARIPMGLRAGPLGTAGRLVAHVANRCAVGTSLMLALAGSLRRLRSRPAL"
     gene            complement(3039..3491)
                     /gene="dut"
                     /locus_tag="AC16JC_09885"
     CDS             complement(3039..3491)
                     /gene="dut"
                     /locus_tag="AC16JC_09885"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006832520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR5165043.1"
                     /translation="MTDRSPVVQVLRLPHHEGLELPARATAGAAGYDVASAEPEFTIR
                     PMERRLVRTGLALAIPAGYEAQVRPRSGLALKHGLTLPNTPATIDSDYRGELLVAVIN
                     LGTEPVTVGRGTRIAQLVFQRVEDVRLEAVDELPPSERGGGGFGSTGT"
     gene            complement(3488..4600)
                     /gene="ald"
                     /locus_tag="AC16JC_09890"
     CDS             complement(3488..4600)
                     /gene="ald"
                     /locus_tag="AC16JC_09890"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013292000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000286 - L-alanine dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0042853 - L-alanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HIR5165044.1"
                     /translation="MIVGVPREIKTAENRVALVPAGAEALIGDGHTVLVEQGAGLGSG
                     FADDAYRAVGATIAGSVDEVWAKAEMILKVEEPIEPEWKRIRKGQVVFTYFHFAASEP
                     LTRAIIASGCVAIAYETVQLPTGELPLLTPMSEVAGRMAVQEGAKYLEKVFGGSGILL
                     GGVPGVLPAEVVVIGGGVVGTNAAKMAAGLGAHVTLLDISLDRLRYLSDVLAPNVDLL
                     YSNRHNILEQIRKADLVIGAVLLPGAKAPNLVKRADLKVMKPGSVIVDVAVDQGGCIE
                     TTKATTHEQPTYVVDGIIHYAVANMPGGVPRTSTLALTNATFPYAKRLAKRGWQQACR
                     DDRSLFLGLNVIEGKVVYPGVAEAFGLPVADPQTLV"
     gene            complement(4626..>5123)
                     /locus_tag="AC16JC_09895"
     CDS             complement(4626..>5123)
                     /locus_tag="AC16JC_09895"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5165045.1"
                     /translation="TDTVRFSDPRKYALMALSNVFGGGMSSRLFQRVREELGLAYAVY
                     SYTHFYGQVGVFGTYVGTQPRTADQALEAIRGEYGKLAREGLTSDALEQAKRQTQGQL
                     TLMLESPTARMYRLASFAVHGEPYRGLDETLAEIGALTPAAAAEVAAEFFAPERQTLV
                     WLGPN"
BASE COUNT          796 a         1799 c         1756 g          772 t
ORIGIN      
        1 tcgcgccctg cccggtggga gcgctggcga tgaccccggc ctgatgcgcg agatcgacat
       61 cctggtggtg ggggccgggc cggccggctc gatcgccgcg ctcgaggcgg cgggtgcggg
      121 cctcgaggtc gtcctgctcg agcgcgatcg ggccgtgggc gctccggtgc gctgcgccga
      181 gggagtgggt gacgccgggc tgcgggagtt cgcggacccc gcaggcgcgc cgtgggcggc
      241 ccgcaagatc acgcgggtga tcttcctcgc ccccgacgac accgaggtcc aggtcgccga
      301 gcgggacgtc ggctggatcc tggaccgcac ccgtttcgat gcccatctcg ccgcccaggc
      361 ggtcgcggcc ggcgcggagc tccaggtcgg ggccgaggcg acgggcatga cgcgcgagac
      421 ggacggccgc tggaccgtcg cggtacgcgg atccgggggt cccgacggtt accgggctcg
      481 cgtcgtcatc ggcgccgacg gcgtggagac gatggtggga cgctgggcgg gcctcgacac
      541 ccgtgtcccc gcccgtgaca tggaatcctg cgcccagtac gtcgtgcagg gcatcgactt
      601 cgatcccgac gcgatctacc tgcagttcgg cgacggcatc gcacccgggg gatacgcgtg
      661 gctgttcccg aagggcgagc gcgtcgccaa cgtcggcctc ggcatggtgg cgctgcacag
      721 cgacggtcgc accgcgcgcc aatacctcga cgcctggatc acccgccgct atccgcgggg
      781 ggcccggacc gcgtacaccg tcggcggggt catcgtgcat accacgatca agcagaccta
      841 tgccgacggc gtgttggtgg cgggcgacgc ggcgcacatg atcaaccccc tctcgggcgg
      901 gggcatcgtc aacgcgatga aggcgggacg gctggcgggc cggacggccg ccgctgcgat
      961 ctgcgcggga gacacgagcg cccgccgcct ggccgggtac caccgcgact ggatgcggct
     1021 cctgggcgac gaccacctca agtactaccg cctcaagcag gcgctcgagc acctggacga
     1081 cgccttcttc aacggcctgg cccggacggt caacgccatc ccccgggaga agcggacgct
     1141 gggacggatc ttcgcgcatg cgctcgtgaa gcatccgcag ttgatcccgg tagcggcgcg
     1201 gttcttcgta taattgcgga ttgcggattt gggatcgcgg atttctccct aaatggatct
     1261 cggtccgtca gtgccaatcc gcaatccgca atccacaatc agtaatcggg gaggggggaa
     1321 atgctgagca tcgacctgac gggcaaacgc gccctggtgg cgggcgtcgc cgacgacgcc
     1381 ggcttcgggt tcgccatcgc gaaggcgctg gccgaggcgg gcgccacggt gtgcgccggc
     1441 acgtggccgc cggcgttgaa catcttccag aatctgctcg agcgcggcaa gatcgacgat
     1501 tcccgccgca tgcccgatgg ctcgctgctc ggtttcgaga agatctaccc actggacgcg
     1561 gcgttcgaca cgctggccga cgcccccgac gatgtgcgga ccaacaaacg ctacaaggac
     1621 gtcggcgact tctcggtcga ggggatacgc cagcggctgg tcgccgactt cggcgagcgg
     1681 ccgctcgaca tcgtcatcca ttcactcgcc aatggcccgg aggtgaagaa gcccctgctc
     1741 gagaccagcc ggtcgggcta cctcacggcc gtcagcgtga gcgcctattc cctcgtgtcg
     1801 ctggtcgccc gcctggctcc gcacctgcgc cccggcgggt cgtttctctc cctgacctat
     1861 ctggccagtg agcgcgtcgt gccggggtat ggcggcggca tgtcgtccgc caaggcggcg
     1921 ctggagagcg acacgcgtac gctcgcctac gaggcggggc gccgctacgg cgtgcgcatc
     1981 aacacgatct cggcggggcc cctggcatcg cgcgcggcca gcgcgatcgg gaagatcgag
     2041 cggatggtgg attactacgc ggccaacgca ccgctggccg agccactccc ggcacgcgac
     2101 gtcggtacgg tcggcgcgtt tctgtgcagt ccactggcga gcggcatcac gggaaccacg
     2161 atctacgtcg acaagggcta ccatacgatg gggatggccg tcgcgggcgt cgaggggcta
     2221 tagggcgggc ctcgaccgca atcggcgcag cgacccggcc agcgccagca tgaggctcgt
     2281 ccccacggcg cagcggttcg cgacgtgggc cacgagtcgc ccggcggtgc ccagcggtcc
     2341 cgcacgcagg cccatcggaa tgcgcgcttt gtcccacatg tacaccggca tccgcggcag
     2401 ccgcaacgga tccgaaccgc tctcccacac tcccgcgaac ccgatctcga acccggcgcg
     2461 ccgcgcgatt tcgacgacgt ggcggttcgc ggccccgaac gggtaggcga ccgcccgccc
     2521 ggcggcggca ccgaaccgcc cgatcagcgt ctcgcgcgag cggccgagct cctcggccag
     2581 agccgcatcc ttcatccagg tcagacggcg gtgggtggcg gaatgggatc caaagtcgaa
     2641 cccccgggcc cgccacgcct cgatggcggg ccagtcgagg tgggtgagcc gcttccgcgt
     2701 gtagcggacg tcccataggt tctggcgccc cacatagtcc gtgaccagaa aggtggtcgc
     2761 cgtgaacccc acgtcggcca ggaccggata ggcatgctcg gccagactct cgtagccgtc
     2821 gtcgaaggtc agcaggaagt tgcgtccggt cgcagaaagc cgcgccacgg ggaacaggta
     2881 gggtgaatcg gcctggatga actgctccag tgtgacggta cgccagccat ccttcgccag
     2941 cgcggtcatg tggcgcgcga acgtttccgg ggtgaccctg gtgatcccca gctccggggg
     3001 accgccgacg cggtggtagc acagcaccgg gggccggatc atgtcccggt gctcccgaac
     3061 cctcctccgc cccgctcgct gggcggcagc tcgtccaccg cctccaaccg cacatcctcg
     3121 actcgctgga acaccagctg ggcgatccgc gtgccgcgtc ctaccgtcac cggctcagtg
     3181 cccaggttga tgaccgcgac gagcagttcg ccgcgatagt cggagtcgat ggtggcgggc
     3241 gtgttgggaa gtgtgagacc gtgtttcagc gccagccccg agcgggggcg cacttgcgct
     3301 tcgtagcccg cgggaatggc cagggcgagc cccgtgcgaa cgaggcggcg ctccatcggc
     3361 cggatagtga actccggctc ggcgctcgcc acgtcgtatc cggccgcacc ggccgtcgcc
     3421 cgcgccggca gctccaggcc ttcgtggtgc gggaggcgga gcacctgcac cacgggggaa
     3481 cgatcggtca caccagcgtc tggggatccg cgacaggaag gccgaacgct tcggccacgc
     3541 ctggatagac gaccttgccc tcgatcacgt tgagtccgag aaacagcgac cgatcgtccc
     3601 ggcacgcctg ctgccagccc cgcttggcga ggcgcttggc ataggggaac gtggcgttgg
     3661 tgagcgcgag ggtagacgtc cgcggcacgc cccccggcat gttggccacc gcgtagtgga
     3721 tgatgccgtc cacgacgtag gtcggctgct cgtgcgtcgt cgccttggtc gtttcgatgc
     3781 agccaccctg atcgaccgcg acgtccacga tcaccgagcc cggcttcatc actttcaggt
     3841 ccgcccgctt cacgaggttc ggggccttcg cgccgggcag caacaccgcc ccgatcacga
     3901 gatcggcttt gcggatctgc tccaggatat tgtgccggtt cgaatacagc agatcgacgt
     3961 tcggcgccag cacgtcggag agatagcgca gccggtcgag cgaaatatcg agcagggtga
     4021 cgtgggcgcc gagcccggcg gccatcttgg ccgcgttggt cccgaccacg cccccgccga
     4081 tcacgaccac ctcggccggg agcactcccg gcacaccgcc cagcaggatg ccgctgcccc
     4141 cgaacacctt ttccaggtat ttcgcgccct cctgcaccgc catgcgcccg gccacctcgc
     4201 tcatcggcgt gagcagcggg agctcacccg tggggagctg cacggtctcg tacgcgatcg
     4261 cgacgcaccc cgaggcgatg atcgcccgcg tgagcggctc cgatgcggcg aagtggaagt
     4321 aggtgaacac gacctgaccc ttgcggatgc gcttccactc cggctcgatc ggctcctcca
     4381 ccttgagaat catctcggcc ttcgcccaca cctcgtccac cgagccggcg atggtggcgc
     4441 ccaccgcgcg ataggcgtcg tcggcgaaac cggagcccaa cccggcgccc tgctcgacca
     4501 gcaccgtgtg cccgtcgccg atcagcgcct cggcccccgc cggcacgagc gccacgcggt
     4561 tttcggccgt cttgatctcc ctgggaaccc cgacaatcat cgtgtgaccc cggatcggtg
     4621 gagcgtcagt tgggtccgag ccagacgagc gtctgacgct cgggagcgaa gaattccgcg
     4681 gcgacctccg cggccgcagc gggcgtcaag gcgccgatct cggcgagggt ctcatccaga
     4741 ccgcgatacg gctcgccgtg tacggcgaaa ctggccaaac gatacatccg cgcggtgggg
     4801 ctctccagca tgagcgtcag ctgcccctgc gtctggcgct tggcctgctc gagcgcgtcg
     4861 gacgtcagac cctcacgggc gagcttcccg tattccccgc ggatcgcctc cagtgcctga
     4921 tccgccgtcc ggggttgggt gccgacgtac gtgccgaaca ctcccacctg gccgtagaag
     4981 tgggtgtagg aatacacggc gtaggccagc ccgagctctt cccgcacgcg ctggaacagc
     5041 cggctcgaca tcccgccgcc gaagacattg gacagcgcca tcagcgcgta cttgcgggga
     5101 tcggagaagc ggaccgtatc ggt
//
LOCUS       DBNGZW010000421         2699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_97282, whole genome shotgun sequence.
ACCESSION   DBNGZW010000421 DBNGZW010000000
VERSION     DBNGZW010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2699
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_97282"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..932
                     /locus_tag="AC16JC_09900"
     CDS             <1..932
                     /locus_tag="AC16JC_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5165046.1"
                     /translation="TPTSETTSRSTRLVGYACALGAGSIWGTTGPLSTALYAQGGTLT
                     GIGFWRLLLGIVGILAFGLLQRDFFRIDLRGWLLVGLGGGALVALFEVAYQFAISGAG
                     VAGAAALLYTAPVLVAVLAVPLLGERLTPTRLVLALVVMAGAALAVLGGSREGVTVAT
                     TLRAGVLGGALAALSFAGTTLLARYAVPRYGPMRVLFLEILGGIAVLGIVLPLAGRPA
                     LPPPTAGAWLYTALLAAGTVVAANVLFFNAAKRIDAAPTAVAATIEPVVGALLAFALF
                     AQRLTPLGWIGLLMVVGGVAGGYLEEAHPDARG"
     gene            934..1599
                     /locus_tag="AC16JC_09905"
     CDS             934..1599
                     /locus_tag="AC16JC_09905"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012014219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR5165047.1"
                     /translation="MSSFIDLSHVVTDGMVTYPGLPAPVIRDHLSREASIGRYAPGTE
                     FQIGRIEMVANTGTYVDAPFHRYAGGTDLAGLDLAALADLSGLVVHAGGSSPAIDRDV
                     FATIAPSGRAVLVHTGWARHWATPTYGAGGHPFLTRAAAEHLVSAGARLVGIDSLNID
                     DATDGTRPAHTILLGAGIPIVEHLTGLERLPDSGFRFFAVPPKVVGMGSFPVRAFALV
                     PST"
     gene            1614..2003
                     /locus_tag="AC16JC_09910"
     CDS             1614..2003
                     /locus_tag="AC16JC_09910"
                     /inference="COORDINATES: protein motif:HMM:NF021612.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HIR5165048.1"
                     /translation="MLDSVLAACLGVVIGLLVGFVYFLIWKARHTGIIRQDAVQRSQA
                     VTVGKVSEQLLPYLPGFRFNPKDARFLGSPVDLVVFDGLAEGDLRGVWFLEVKTGNAV
                     LSARERQIRDAILEKRVGWDELRLPRG"
     gene            complement(2040..>2699)
                     /locus_tag="AC16JC_09915"
     CDS             complement(2040..>2699)
                     /locus_tag="AC16JC_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165049.1"
                     /translation="NAFQLLRRIRFDSLTGDTINPARVEYPLDYDRRHSLTVVGQARV
                     SDEAGPRLLGSSLFGGLEAAAIFHFGSGLPYSRTNTAGDTLIGLPNSYRLPPEYSLDA
                     LVRRPLRLWGMSGGVYLDARNIFNRRNTVAVRRDTGQPGLTDATLQSIAQAAYQEHPE
                     PIPYESQRYRPWADQDGNGLIEGPGELMPLYLAAARDFAQPLFYYGSPRLVRLGVELT
                     F"
BASE COUNT          342 a          948 c          953 g          456 t
ORIGIN      
        1 tgactccgac ctcggagacg acgtcccgct cgacgcgcct cgtgggctac gcctgcgcgc
       61 tcggcgcggg atccatctgg ggcacgaccg gccctctctc gaccgcgctg tacgcccagg
      121 gcggcacact caccggcatc ggcttctggc gactcctgct cggcatcgtc gggattctcg
      181 cgttcggact gctgcagcgc gacttcttcc gcatcgacct gcgcggctgg ctgctggtcg
      241 ggctcggcgg aggcgcgctg gtggcgctgt tcgaggtcgc ctaccagttc gcgatctccg
      301 gcgcgggtgt cgccggggcg gcggcgctgc tgtacaccgc ccccgtgctc gtggccgtgc
      361 tggccgtacc actgctgggg gagcgtctca cgccgacgcg tctcgtcctg gcgctcgtcg
      421 tcatggccgg cgccgctttg gcggtgctcg gcgggagtcg cgagggcgtc accgtcgcca
      481 ccacgctgcg cgccggtgtc ctaggcggcg cgctcgccgc gctgtctttc gccgggacga
      541 cgctgctggc ccgctacgcc gtgccgcgct acggcccgat gcgggtgctg ttcctcgaga
      601 tcctgggcgg catcgcggtg ctcggcatcg tgctgcccct cgccggccgc cctgccctgc
      661 caccgcccac agccggcgcc tggctctaca ccgcgctgct cgccgcgggc accgtggtgg
      721 ccgcgaacgt gttgttcttc aacgccgcta agcggatcga cgcggccccg acggcggtcg
      781 ccgcgacgat cgagccggtg gtgggcgccc tgctcgcctt cgccctcttc gcgcagcggc
      841 tcacgccgct cggctggatc ggattgctca tggtggtcgg cggcgtggcc ggcggctacc
      901 tcgaggaggc ccaccccgac gcgagaggct gacgtgtcat cgttcatcga tctgagccac
      961 gtcgtgacgg acgggatggt gacctatccc ggtctccccg caccggtcat ccgcgaccat
     1021 ctgagccggg aggcctcgat cgggcgctac gcgccgggga ccgagttcca gatcggccgc
     1081 atcgagatgg tggcgaacac gggcacgtac gtggacgcgc ccttccaccg ctacgccggc
     1141 gggaccgatc tcgccggatt ggacctcgcg gcgctcgcgg atctctcggg gctggtcgtc
     1201 cacgcgggcg ggtcctcgcc ggcaatcgac cgcgatgtgt tcgccacgat cgcgccgagc
     1261 ggccgcgcgg tgctggtgca cacgggctgg gcccggcact gggcgacccc gacgtatggt
     1321 gcgggcggac atccgttcct cacgcgcgcg gccgcggagc atctggtgag cgctggcgct
     1381 cgactcgtgg gcatcgactc gctgaacatc gatgacgcga ccgacggcac ccgtcccgcg
     1441 catacgatcc tgctcggcgc cgggattccc atcgtggagc atctcacggg gttggagcgg
     1501 ctgccggaca gcgggttcag gttcttcgcg gtgccgccga aggtagtggg gatgggctcg
     1561 ttcccggtgc gcgcgttcgc gctggtgcct tccacctaat tatggagagg gacgtgctcg
     1621 attccgttct cgccgcgtgc ctcggcgtcg tcatcggatt gctggtcggc ttcgtctact
     1681 tcctgatctg gaaggcgcgc cataccggca tcatccgcca ggacgccgtc cagcgcagcc
     1741 aggcggtgac cgtaggcaag gtctccgaac agctcctgcc ctatctgcct gggttccgct
     1801 tcaaccccaa ggacgcgcgg ttcctgggca gtccggtcga tctcgtggtg ttcgacgggc
     1861 tggccgaggg cgatctgcgc ggcgtgtggt ttctagaggt gaagaccggg aatgcggtcc
     1921 tgagcgcccg cgagcgccag atccgcgacg ccatactcga gaagcgcgtg gggtgggacg
     1981 agctgcgcct cccccgcggc tgacctccac caacctccac cgtcctccac cgtcctcccc
     2041 taaaacgtca gctcgactcc caggcgcacc agccgcggcg atccgtagta gaacagcggc
     2101 tgcgcgaagt cccgcgcggc ggcgagatac agcggcatca gctcgcctgg accctcgatg
     2161 agcccattgc cgtcctggtc ggcccacggg cggtagcgct gcgactcata ggggatcggc
     2221 tcgggatgtt cctgatacgc cgcctgcgcg atcgactgca acgttgcgtc ggtcagcccc
     2281 ggctgcccgg tgtcgcgccg caccgcgacc gtgttgcggc ggttgaagat gttccgggca
     2341 tcgaggtaga cgccgccgct catgccccag aggcgcaacg ggcgtcgcac cagcgcgtcg
     2401 agcgaatact ccggggggag acggtacgag ttgggcaggc cgatcagcgt gtcgcccgcg
     2461 gtgttggtcc gcgagtacgg gaggcccgat ccaaagtgga agatggccgc ggcttccagt
     2521 ccgccgaaca gggagctgcc gagcaggcga gggcccgcct cgtcgctcac ccgcgcctgg
     2581 cccaccacgg tgaggctgtg gcgccggtcg tagtcgagcg ggtactccac gcgggccggg
     2641 ttgatggtat cgccggtcag ggaatcgaac cggatgcggc gcagcagctg gaaggcgtt
//
LOCUS       DBNGZW010000422         5013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1674165, whole genome shotgun sequence.
ACCESSION   DBNGZW010000422 DBNGZW010000000
VERSION     DBNGZW010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5013
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1674165"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..1277)
                     /locus_tag="AC16JC_09920"
     CDS             complement(45..1277)
                     /locus_tag="AC16JC_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012825392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HIR5165050.1"
                     /translation="MDERELIHDWNRVGASFDWSRARVDLNDETLRDGLQSPSVKDPS
                     LEIKRKLLHLMADLGIAAADIGLPGAGPRVVEQVRALAREIRDHKLPIFPNCAARTVI
                     ADIEPVARISQEIGIPIEAATFIGSSPIRQYTEDWTIERMLKATEDAVTFAVRQGLPV
                     MYVTEDTTRAKPETLKRLYGTAIDCGATRICLADTVGHATPDGVQALVAFIKQEIVQK
                     KDVKIDWHGHRDRGMGLVNCLAAIEAGVDRVHATALGVGERVGNAEMDLLIVNLKLLG
                     AHKGDLARLPEYCRTVAEAVGIPIPLNYPVMGSDAFRTGTGVHAAAIIKAKKKGHNWL
                     ADRVYSSVPAAELGLEQVIEISPVSGLSNLKFWLQHHGYDPDDEAACQALFDAAKRAD
                     HTLTAEECHSLLRQGAWR"
     gene            complement(1309..1941)
                     /locus_tag="AC16JC_09925"
     CDS             complement(1309..1941)
                     /locus_tag="AC16JC_09925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015195753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5165051.1"
                     /translation="MAIPGYKRLVPWLLARANGPYERWMADRKRNLFGALRGVVVEIG
                     PGAGANLPYLRSDVRWIGIEPNPYARPYLERAAREAGVAHELREGDAERLPVDAASAD
                     AVIGTLVLCSVNDPGAALREIQRVLKPGGRYAFIEHVAAPAGTWRRRVQRWVRPAWRV
                     VADGCNPDRETLKAIRAAGFARVDAEEFLAPAGLVAPHIAGVAVKDGTSA"
     gene            1989..2963
                     /locus_tag="AC16JC_09930"
     CDS             1989..2963
                     /locus_tag="AC16JC_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012037918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HIR5165052.1"
                     /translation="MVRLHNMEKLVRGLHHVTATVNDAQQDFDFYAKVLGLRLVKKTV
                     NFDNHNVYHFYYGNEAGAPGTIMTTFPYKGWGVPVGQKGAGQIRVTSFSVPAGSLDFW
                     KARLRDGGATVQDEVPRFGEESITVTDPSGLILELVASAGDARSPWVPDDVPAASAIR
                     GIHSITMPIRAPARTLELMTQVLGFDVVNEGPDRTRLAVGGDLPGNLIDVVHASDIPP
                     GINGLGTVHHVAMAVSDDEEQLRMRAELVRLGYQVTPVMDRQYFRSIYFREPGGVLFE
                     LATVPPGFTIDETLPCLGQDLKLPPWEEPNRTEIAAKLPPIVAVSPRA"
     gene            3032..3601
                     /locus_tag="AC16JC_09935"
     CDS             3032..3601
                     /locus_tag="AC16JC_09935"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013179116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5165053.1"
                     /translation="MVHGRNAGPANILDLVSRLGRPDLTYLAPAAAGRTWYPLSFMAE
                     KEKNEPGLSSGLWVLGQLVQDIVARGISRDKIILLGFSQGACLTAEFAVEHAARYGGV
                     ILYSGGLIGPPGTTWEYPGSLDGTPIFLGCSDVDAHVPKTRVDESAAVFTRMGATVTE
                     RIYPGMGHLVNDDEIAFTRGVLDAIAYRA"
     gene            complement(3589..3960)
                     /locus_tag="AC16JC_09940"
     CDS             complement(3589..3960)
                     /locus_tag="AC16JC_09940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008922350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5165054.1"
                     /translation="MSAATPVEVILRRFESPDETRVMQKGKFELVRIGGLTIGRATYE
                     PGWRWSEHVGPTVGAKRCSVEHVGLVLAGTATAALEDGRVFELRAGELFYIPPIPHDS
                     WVVGDEAYVSLHFLGADHYAR"
     gene            complement(3957..4649)
                     /locus_tag="AC16JC_09945"
     CDS             complement(3957..4649)
                     /locus_tag="AC16JC_09945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR5165055.1"
                     /translation="MQATEFEFRHRFWVIFTVFAAGFAAYVVDRHNTATALAHGSRTG
                     AQVILGAGALLAAAAAALRTWASAFLRTPVVKDRQLHADRLVADGPYRYLRNPLYLGT
                     LALAVCFGLMASRVGFFVIVGGLLIVVYRLIGREEAALARSQGDAYTAYRARVPRLWP
                     AITPRVPASGARAAWGQALAGELMMWAFALAAAAFAVTLDRRLFYAFLVLGFIGYLPG
                     VRTIIAPSRSHP"
     gene            complement(4693..>5013)
                     /locus_tag="AC16JC_09950"
     CDS             complement(4693..>5013)
                     /locus_tag="AC16JC_09950"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019881845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR5165056.1"
                     /translation="LSAGPDPYAAMAGAPDSDVRVLLAHQPRSAYDARAAGFDLQLSG
                     HTHGGQFFPFNLLVRLFQPFVSGLHRLEEMWLYVSRGTGYWGPPLRLWAPSEITLIEL
                     TAAG"
BASE COUNT          804 a         1796 c         1651 g          762 t
ORIGIN      
        1 ccagcgcaga tcgaagtacg accgctcgag ctcccggaac gcgctcagcg ccatgcgccc
       61 tgccgcagca ggctgtggca ttcctccgcg gtcagcgtgt ggtcggcccg cttcgcggcg
      121 tcgaacagcg cctggcacgc cgcctcgtcg tcggggtcgt acccgtgatg ctgcagccag
      181 aacttcaagt tggagaggcc cgacaccggc gagatctcga tcacctgctc gagccccagc
      241 tccgcggccg gcaccgagct gtagacccgg tccgccaacc agttgtggcc cttcttcttc
      301 gccttgatga tcgccgccgc gtggacgccg gtgccggtgc ggaacgcgtc gctccccatc
      361 accggatagt tcaacgggat ggggatgccc accgcctcgg ccacggtgcg gcaatactcc
      421 ggcaacctcg cgaggtcgcc tttatgggcg ccgagcaact tgaggttcac gatcagcagg
      481 tccatttccg cgttccccac ccgctcgccc acgcccagcg ccgtggcatg cacccggtcc
      541 actcccgcct cgatcgccgc gagacagttg accaacccca tcccccgatc ccgatgcccg
      601 tgccagtcga tcttcacgtc cttcttctgg acgatctcct gcttgatgaa ggcgaccaac
      661 gcctgcacgc cgtccggcgt cgcgtgtccc acggtgtccg cgagacagat cctcgtcgcc
      721 ccacagtcga tcgccgtccc gtacagccgc ttcagggtct cgggcttggc ccgggtggtg
      781 tcctcagtga cgtacatgac cggaagcccc tgccgtaccg caaaggtcac cgcgtcctcg
      841 gtggccttga gcatccgctc gatcgtccag tcctcggtgt actggcggat cggcgacgag
      901 ccgatgaagg tcgccgcctc gatcgggatg ccgatctcct gggagatccg cgcgaccggc
      961 tcgatatcgg cgatgaccgt acgggccgcg caattcggga agatcggcag cttgtggtcc
     1021 cggatctccc gggcgagcgc ccgcacctgc tccaccacgc gcggcccggc gccgggcagg
     1081 ccgatgtcgg cggcggcgat gcccagatcg gccatcaggt ggagcagctt gcgtttgatc
     1141 tcgagggagg ggtccttcac cgacggtgac tggagaccgt cgcgcagcgt ctcgtcgttc
     1201 aggtccaccc gggcacggga ccagtcgaac gacgccccga cgcggttcca atcgtggatc
     1261 agctcccgtt cgtccatgcg cgtagtgtcg cccggttcgt cgcagtggtc aagccgaggt
     1321 cccgtccttc acggcgaccc cggcgatatg gggggcgacg agcccggccg gcgcgagaaa
     1381 ttcctctgcg tcgacgcgcg cgaacccggc cgcgcgaatc gccttcagcg tttcccggtc
     1441 gggattgcag ccgtcggcga ccacgcgcca ggcgggccgc acccagcgct gcacgcgccg
     1501 ccgccacgtc cccgccggcg ctgccacgtg ctcgatgaac gcgtagcgcc cacccggctt
     1561 cagcacgcgc tgaatctcgc gcaacgccgc tccggggtca ttcaccgagc agagcaccaa
     1621 cgtaccgatc acggcatccg cgctcgccgc gtccactgga agccgctccg cgtccccctc
     1681 ccgcagctcg tgcgccacgc ccgcttcccg ggccgcgcgc tccaggtacg gccgcgcgta
     1741 gggattcggc tcgatgccga tccagcgcac gtcgctcctc aagtacggca ggttggcccc
     1801 ggcgccaggc ccgatttcga ccaccacgcc gcgcagcgcg ccgaacagat tgcgcttgcg
     1861 gtcggccatc cagcgctcat agggcccgtt ggcccgcgcg agcaaccagg ggacgagccg
     1921 cttgtagccc gggatcgcca tgcgagcagc atcaccatcc tccccctgcc ggtcaagccg
     1981 agttcgccat ggttagattg cacaacatgg agaaactcgt gcgcggcctg catcacgtga
     2041 cggcgacggt gaacgacgcc cagcaggact tcgacttcta cgcgaaggtc ctggggctgc
     2101 ggctcgtgaa gaagaccgtc aacttcgaca atcacaacgt ctaccacttc tattacggca
     2161 acgaagccgg ggccccgggc accatcatga cgaccttccc gtacaaggga tggggcgtgc
     2221 cggtggggca gaaaggcgcg ggacagatcc gcgtcacgtc gttctcggtc ccggccggat
     2281 cgctcgactt ctggaaggcg cggctgcgcg acggcggcgc gacggtccag gacgaggttc
     2341 cgagattcgg ggaagaatcg atcaccgtca ccgacccgtc gggcctgatc ctcgagctcg
     2401 tggcgagcgc cggcgatgcg cgatccccgt gggtgccgga cgacgtgccg gccgccagcg
     2461 ctattcgcgg catccacagc atcacgatgc ccatccgtgc acccgccaga acgctggagc
     2521 tgatgaccca ggtgctcggg ttcgacgtcg tgaacgaagg ccccgaccgg acccggctgg
     2581 cggtgggcgg cgatctcccg ggaaacctca tcgacgtcgt ccacgcgtcc gacatccctc
     2641 caggcatcaa cggcctgggc accgtgcatc acgtcgccat ggccgtgagc gacgacgagg
     2701 agcagctgcg gatgcgggcc gagctggtgc gcctcggcta ccaggtgact cccgtgatgg
     2761 accggcagta tttccgctcg atctacttcc gcgagccggg cggcgtgctg ttcgagctcg
     2821 ccaccgtccc gcccgggttc accatcgacg agaccctgcc ctgcctcggc caggatctga
     2881 agctgccgcc ctgggaggag cccaaccgga ccgagatcgc ggcgaaactg ccgcccatcg
     2941 tcgccgtgtc tccccgggcg tgaagcccca cgcgggagag cctctcggga gcgccgggag
     3001 gccgctgggg gagagtcccg ccgtcgtgat catggttcat ggccgcaacg ccggacccgc
     3061 gaacatcctc gacctcgtgt cacggctggg acgtcccgac ctcacctacc tggccccggc
     3121 cgccgccggg cggacctggt atccgctgag cttcatggcg gagaaggaga agaacgagcc
     3181 agggctgtcg tccggcctct gggtgctggg gcaattggtg caagacatcg tcgcgcgggg
     3241 aatcagtcgc gacaagatta tcctgctcgg gttttcacag ggcgcgtgcc tgacggccga
     3301 gttcgccgtg gagcatgcgg cgcgctacgg cggcgtgatc ctctacagcg gcggactgat
     3361 cgggccgccc ggcaccacct gggagtatcc cgggtcgctc gacggcactc cgattttcct
     3421 gggatgcagc gatgtggacg cgcatgttcc caagacgcgc gtggatgaaa gcgccgcggt
     3481 cttcacccgt atgggtgcga ccgtcaccga gcgcatctat cccgggatgg gccacctggt
     3541 gaacgacgac gagatcgcgt tcacgcgggg agtgttggac gcgatcgcct accgcgcgta
     3601 gtggtcggcc cccaggaaat gcagcgagac gtaggcctcg tctcccacga cccagctgtc
     3661 gtgcgggatc ggcgggatgt agaacagctc ccccgcccgc agctcgaaca ctcggccgtc
     3721 ctcgagcgcc gcggtcgccg tgccggcgag gacgaggccc acgtgctcca ccgagcaccg
     3781 tttcgctccc accgtcggcc cgacgtgctc ggaccagcgc caccccggct cgtaggtcgc
     3841 gcgcccgatg gtgagcccgc caatcctgac gagctcgaac ttgcccttct gcatcacgcg
     3901 ggtctcgtcg ggcgactcga accgccgcag gatgacctcg acgggagtgg ccgcactcat
     3961 ggatggctcc gcgaaggcgc gatgatggtt cgcacgcccg gcaggtagcc gatgaatccc
     4021 aggaccagga acgcgtagaa gagtcgtcga tcgagcgtca ccgcgaatgc cgccgccgcc
     4081 agcgcgaagg cccacatcat gagctccccg gccaacgcct gcccccacgc cgcgcgcgct
     4141 ccgctggcgg ggacccgggg cgtgatcgcc ggccacagcc gggggacgcg ggcccgatat
     4201 gccgtatagg cgtcgccctg gctgcgcgcc aatgccgcct cttctcgccc gatcaaccgg
     4261 tagacgacga tcagcaggcc tccgacgatc acgaagaacc ccacccggct cgccatcaac
     4321 ccgaagcaga cggcgagcgc cagcgtcccc agatagagtg gattgcggag atagcggtag
     4381 gggccgtccg cgacgagacg gtcggcgtgc agctgtcggt ctttcacgac cggcgtccgc
     4441 agaaacgcgc tggcccaggt tcgcaacgca gcggccgcag cggccagcaa ggcgccggcg
     4501 ccgagtatca cctgtgcccc ggtccggctg ccgtgggcga gcgcagtggc cgtattgtga
     4561 cgatcgacca cgtaggcggc gaacccggcg gcgaatacgg tgaagatcac ccagaagcgg
     4621 tggcggaatt cgaactcggt cgcctgcatg ggtcgagact acgggtcgcg tggcgtgcgg
     4681 tttcgccgcc gctcaaccgg cggccgtgag ctcgatgagg gtgatctccg acggtgccca
     4741 gagtcgcagc gggggtcccc agtaccccgt gccgcggctc acgtacagcc acatctcctc
     4801 caaccggtgc agcccggaga cgaacggctg gaacaggcgc accagcaggt tgaaggggaa
     4861 gaactggccg ccgtgtgtgt ggcccgagag ctggaggtcg aatcccgccg cccgcgcgtc
     4921 gtacgcgctg cggggctgat gcgccagcag cacccgcacg tcgctgtcgg gcgcgcccgc
     4981 catggccgcg tacggatccg gtcccgcgct gag
//
LOCUS       DBNGZW010000423         3060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_312285, whole genome shotgun sequence.
ACCESSION   DBNGZW010000423 DBNGZW010000000
VERSION     DBNGZW010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3060
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_312285"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="AC16JC_09955"
     CDS             complement(<1..593)
                     /locus_tag="AC16JC_09955"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR5165057.1"
                     /translation="MNSGPNATKYIFVTGGVVSSLGKGIAAASLGRLLVERGLSVTMQ
                     KFDPYINVDPGTMSPFQHGEVFVTDDGAETDLDLGHYERFIDRSLSQQNNITTGRIYQ
                     TVINKERRGEYLGSTVQVIPHITDEIKNAIRRTAPGHDVVITEIGGTVGDIESLPFLE
                     AIRQFRQEVGRDNALFMHLTLVPYIAAAGELKTKPTQ"
     gene            complement(598..1341)
                     /gene="kdsB"
                     /locus_tag="AC16JC_09960"
     CDS             complement(598..1341)
                     /gene="kdsB"
                     /locus_tag="AC16JC_09960"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIR5165058.1"
                     /translation="MRVLGVIPARLGSTRLPNKPLQLLAGEPLITRVIQHVVDLGLVE
                     QLVVATDSQMIAQVVERAGVRAVLTHDGHVAGTDRVAEAAGRAEFAAFDVVANVQGDE
                     PFLPREALAGAIERVAQGDDIGTAAAPLAAEQAADPSRVKVVTDAGGRALYFSRAAIP
                     YRREPTDPAEGLYWQHLGLYVYQRAALERWVASPPSRAEQAERLEQLRALHCGLTIGV
                     ARLTEPALPGVDTPDDLRRAEAHWHALQG"
     gene            complement(1414..2052)
                     /locus_tag="AC16JC_09965"
     CDS             complement(1414..2052)
                     /locus_tag="AC16JC_09965"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017503945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR5165059.1"
                     /translation="MLLVLDNYDSFTYNLVQYAGELGADPVVHRNDALGVDDALALEP
                     AAIVISPGPCTPREAGISVPLVRAAAAAGVPLLGVCLGHQAIGEAFGGDVVRADRLMH
                     GKTTMVAHTGHPLFDGLPSPFEAMRYHSLVVAPATLPGELAVTAWSEDRPAGSEIMAL
                     SHRELPVYGVQFHPESVATDHGKRILFNFLALAAPARAGAGHHPVTASYTTP"
     gene            complement(2065..2796)
                     /locus_tag="AC16JC_09970"
     CDS             complement(2065..2796)
                     /locus_tag="AC16JC_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165060.1"
                     /translation="MRSYWLRILLGALAVFAIGMIGVTLVRHTKQTVTAVVTGSGPLS
                     IPLPFVPFELEGSKLGELKRLVVNRDAPKKVSSVELEVKLDDSLLVQGLAGCRLAANL
                     ESDSTKPGDINLHPSRTNDQAFFFCAKSDSGLVEFGTVHLSPGDLVVPLLVPVSLAED
                     LRAGTWTSHDSADSADILAQRAESLAGKAEQAAESLAARQEQRAEAARVTGSRLGDSL
                     RLVGKRRADSVRTAMARMADSVGAR"
     gene            2912..>3060
                     /locus_tag="AC16JC_09975"
     CDS             2912..>3060
                     /locus_tag="AC16JC_09975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5165061.1"
                     /translation="MPAGRGPQVIEVEGLEKLYGDFPAVRGLSFQVQPGEVLGLVGPN
                     GAGKTT"
BASE COUNT          475 a         1037 c         1077 g          471 t
ORIGIN      
        1 tgctgggtgg gtttggtctt gagctcgccg gccgcggcga tgtagggcac cagggtgagg
       61 tgcatgaaga gtgcgttgtc gcggcccact tcctggcgga actggcggat cgcctccagg
      121 aacggcagcg actcgatgtc gcccaccgtc ccgccgatct ccgtgatcac gacgtcgtgg
      181 cccggcgcgg tgcggcggat ggcgttcttg atctcgtcgg tgatgtgggg gatgacctgc
      241 accgtggaac cgaggtactc gccccgccgc tccttgttga tcacggtctg gtagatgcgc
      301 ccggtggtga tgttgttctg ctgggagagc gagcggtcga tgaagcgctc atagtggccg
      361 aggtcgaggt cggtctccgc cccgtcgtcg gtcacgaaca cctcgccgtg ctggaacggc
      421 gacatggtgc cggggtccac gttgatgtac gggtcgaact tctgcatcgt cacgctgagg
      481 ccgcgctcga cgagcaggcg cccaagtgac gcggcggcga tgcctttccc gagggaagag
      541 acgacgccgc cggtgacgaa gatgtacttg gtcgcgttcg gacccgagtt catatggcta
      601 cccctgtagt gcgtgccagt gggcttccgc ccgtcgaaga tcgtccggcg tgtccacgcc
      661 gggcagcgcg ggctccgtca gccgggccac gccgatcgtg agaccgcagt gcagcgcgcg
      721 aagctgctcc agtcgctcgg cctgctccgc ccggctcggc ggcgaggcga cccaccgctc
      781 gagcgccgcg cgctggtaga cgtagagccc gagatgctgc cagtagagtc cctcggccgg
      841 gtcggtcggc tcccggcggt atggaatggc cgcgcgcgaa aagtacaggg cccggccgcc
      901 ggcatccgtc accactttga cccgggacgg atcggccgcc tgctcggccg cgagcggggc
      961 cgccgccgtt ccgatatcgt ctccctgggc cacccgctcg atggcgcccg ccaatgcctc
     1021 gcgcggaagg aagggctcgt cgccctggac gttggccacc acatcgaacg ccgcgaactc
     1081 ggcgcgcccc gctgcttcgg ccacccggtc ggtgccggcc acatggccgt catgggtcag
     1141 cacggcccgc actccggccc gctctaccac ttgggcgatc atctgcgagt cggtggcgac
     1201 caccagctgc tcgaccaggc cgaggtccac gacgtgctga atgacccggg tgatgagcgg
     1261 ctcaccggcc agcaactgga gcggtttgtt ggggagacga gtcgagccta gacgagccgg
     1321 aatgacaccc aggacgcgca tcacggaagg taatcgaggg gcggggtcca gtcaaaagtc
     1381 atgccgggca actcccggcc gccctgcctg ccgctatggc gtggtatacg aggcggttac
     1441 ggggtgatgg ccggccccgg cccgggccgg cgctgccagc gccaggaagt tgaagagaat
     1501 ccgctttccg tggtcggtcg ccaccgactc cggatggaac tgcacgccat agacgggcag
     1561 ctcccgatgg ctcagggcca tgatctcgct cccggccgga cggtcctccg accaggcggt
     1621 caccgccagc tcgccgggaa gcgtcgcggg cgccaccacc agggagtggt atcgcatcgc
     1681 ctcgaacggt gacggcaacc cgtcgaacag ggggtgaccg gtatgggcca ccatcgtggt
     1741 cttgccgtgc atgagccggt cggcccgcac gacatcgccg ccgaaggcct cgccgatggc
     1801 ctgatggcca aggcagacgc cgagcagcgg cacccctgcc gcggccgccg cgcggacgag
     1861 ggggacactg atccccgcct cccgcggcgt gcacgggccg ggagagatca cgatggccgc
     1921 cggctcgagc gcgagcgcgt cgtccacgcc caacgcgtcg ttccggtgga ccaccggatc
     1981 ggcgcccagc tcgccggcgt actggacgag gttgtaggtg aagctgtcgt agttgtcgag
     2041 gacgaggagc atgatcctcg aagcttagcg cgccccaacg gagtccgcca tgcgggccat
     2101 cgccgtgcgc accgagtccg cgcgccgctt gcctacgagg cggagcgaat cgcccagacg
     2161 cgagcccgtg acccgcgccg cttcggcacg ctgctcctgc cgtgccgcga gcgactcggc
     2221 ggcctgctcg gccttccccg ccagcgattc ggcccgctgg gcgaggatat cggcggaatc
     2281 ggcgctgtca tggctggtcc aggtgccggc gcgcaggtcc tcggcgagtg agaccggcac
     2341 cagcagcggc accaccagat cccccggcga caggtggacg gtgccgaatt cgacgagccc
     2401 ggagtcgctc ttggcgcaga agaagaacgc ctgatcgttc gtccggctcg ggtgcaggtt
     2461 gatgtcgccg ggcttggtcg agtcgctctc gaggttggcc gcgagccggc accccgccag
     2521 tccctggacc aggagcgaat cgtcgagctt cacctcaagc tccaccgagg acaccttctt
     2581 gggggcgtcg cgattgacca ccagccgctt gagctccccc agcttgctgc cctcgagctc
     2641 gaacgggacg aacggcagcg ggatcgaaag cggccccgag cccgtgacca cggcggtgac
     2701 cgtctgcttc gtgtgccgga cgagcgtgac cccgatcatg ccgatggcga acacggccag
     2761 ggccccgagc aggattcgca accagtagct tcgcatacgg ttcggcctcc agggccgctt
     2821 gcaggggttc cgcacccacc aaggggggta tgttgcaacc agagacgttc cgggtccgtc
     2881 gaaagtttca cccgcgggcg cccaagcggg agtgccggct ggccgaggac cccaggtgat
     2941 cgaggttgaa gggctggaga agctctatgg agactttcct gccgtccggg gcctctcctt
     3001 ccaggtgcag cccggcgaag tcctcggcct ggtgggaccg aacggcgcgg gcaagacgac
//
LOCUS       DBNGZW010000424         4650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1266253, whole genome shotgun sequence.
ACCESSION   DBNGZW010000424 DBNGZW010000000
VERSION     DBNGZW010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4650
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1266253"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1758
                     /locus_tag="AC16JC_09980"
     CDS             <1..1758
                     /locus_tag="AC16JC_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015100088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5165062.1"
                     /translation="AASASTRVDVGGPLSFMPAGACHMSTMRRFPGLPRSLRPALLGG
                     ALVVACTVSDQTGPNSDITDLQITPSSVALATSQSVQLRARGHTKGGGTRDVQVSWSA
                     TGGTIDGNGLYTADTSVGDVDVTATLDSPHLTASAKIRVRGSLKQIVLVPTDATLGTT
                     GTLQFQAYGLTANGDSVDITPTYTATSGTISPEGRYTAPASAGTAQVIATAVVRRNGQ
                     PVADTTQVTITSGTTVPVASVDVTPSSATIAVNATVQLTATPKDAGGNPLSGRTITWT
                     TNNAGVATVSASGLVSGVAAGSATITATSETKSGMAAITVTSGTTGGGVVFVGAGDIS
                     DCGNNNDEATAQLLDGIAGTVYTLGDNAYSSGTATEFSQCYDPTWGRHKARTKPAPGN
                     HDYNTSGASGYYGYFGTLAGPSGRGYYSFDLGDWHIISLNSEVSMSTGSAQETWLRAD
                     LAASTKQCTLAYWHKPRFSSGTNHGSQSSAQPLWQALYDFHAEIVLGGHEHNYERFAP
                     QTPTGAADPTNGIREFVAGTGGESHYNDLGTPLANSEVFNGTTFGVLKLTLGAGTYTW
                     QFIPVAGQSFTDSGSGTCH"
     gene            1859..4366
                     /locus_tag="AC16JC_09985"
     CDS             1859..4366
                     /locus_tag="AC16JC_09985"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5165063.1"
                     /translation="MPHLHKLSVRQALLRGIAGLCVAGCTVPDQNVAGPGTVSQILVL
                     PESITVDPGQALQFRAVGLTTTGATEPVGVRWMATGGGKIDDNGIYVADDHPGHFEVR
                     AIVDRPNLVAGAGVKNRGSLKQVVLNPSSANVPPGGQAQVSASGTLANGDVVPVTVTY
                     AATYGSVTAYGLYTAGQSEGTYQLMAIAGSGNKALADTATIAVSSNAPPTLTRVVLEP
                     PEAAVAAGGTQQFAAYGRLSSGDSVAVSVTYTATGGTVTSSGLYTAGQTTGSYRVVAT
                     ESGGAVADTSAVTIVTGSPTVTSVVLVPASATLTAGGTQQLTAYARLSNGDSGAVSVT
                     YIASGGTITGDGLYTAGQTAGTYSVIAVQAGGTLADTSSVLITSSPVASVQVTPSDAT
                     LAIGTGLQLQVTLKDANGTTLSGRTVAWASSNTAAATVSAGGAVLAVDAGAATVTATC
                     EGKSGTATITVTAPSSGCTGSWPAAVYPRMPPSTGQAFYVSSASGSDANPGTVTAPWR
                     TLQKAFDALQPGQIAYLREGTYGAFWSSSLFSRAGTASAPITVRGYPGERAVLHGQIR
                     LEGSYFRLAHVVVEGPSCGTWGASTQQGDNLVLMVPGTTHHVEVSNSEVYHGGWHAGI
                     AAGGDDIWILNNYIHDNGGFNDPNQYNTSHGIYYHDGTRGVVANNILEHNRAKGLSAR
                     YSANHVIVANNTVVGNGRSGMDIAENTHDWLFANNIVANNGNVNDGVGIHTSGSSGGT
                     SNIEINNVLWNNGVSGTSNWDTDATIINNKVADPLLVSPVSSVPSTTHEGFTNDYHVR
                     SGSPAIDFGSATYALPFDLGGVCRPRGAGPDAGVYEY"
BASE COUNT          820 a         1579 c         1568 g          683 t
ORIGIN      
        1 gccgccagcg cttcgacacg ggtcgacgtg ggcggcccgt tgtctttcat gccagcggga
       61 gcgtgtcaca tgtccaccat gcgcagattc cccggtctcc cgaggtcgct ccgtcccgcg
      121 ctgcttggcg gcgcgctggt cgtggcctgc acggtgtccg accagaccgg tccgaattcg
      181 gatatcaccg atctccagat cacccccagc tcggtcgccc tcgcgacgag ccagtcggta
      241 cagttgcggg ccaggggcca tacgaagggg ggcggcacgc gtgacgtcca ggtgagttgg
      301 agcgcgaccg gcggtacgat cgacgggaac gggctctaca ccgccgatac gtcggtcggt
      361 gatgtcgacg tgacggccac gctcgacagt ccgcacctca cggcctcagc caagatccgc
      421 gttcgcgggt cgttgaaaca gatcgtcctg gtgccgaccg atgcgacgct cgggacgacg
      481 ggcacgctgc aattccaggc atacgggttg accgccaacg gcgacagcgt ggacatcacg
      541 ccgacgtata cggcgacgag tgggaccatc agcccggagg ggaggtacac cgcgccagcg
      601 tccgcaggca ccgcccaggt gatcgcgacg gcggtggtgc gaaggaacgg gcagccggtc
      661 gccgacacca cccaggtcac gatcacgagc gggaccaccg tgcccgtggc atcggtggac
      721 gtgaccccgt cctcggcgac catcgcggtg aacgcgaccg tgcagctgac cgcgacgccc
      781 aaggacgccg gtggcaaccc gctcagcggt cgcacgatca cctggacgac caacaatgcg
      841 ggcgtcgcca cggtgagcgc gagcggactc gtctccggag tggcggcagg atcggcgacc
      901 attacggcga cgtcggagac gaagagcggg atggcggcga tcacggtcac gtccgggacg
      961 acgggcggcg gcgtcgtgtt cgtcggtgcg ggcgacatct ccgactgcgg caacaacaac
     1021 gatgaggcca cggcgcagct gctcgacggc atcgccggaa cggtctacac gctgggcgac
     1081 aacgcgtact cgagcggcac cgcaacggaa ttctcgcagt gctacgaccc cacgtggggg
     1141 cgccacaagg cgcgtacgaa gcccgcaccg gggaatcacg actacaacac gagcggagcg
     1201 tcgggctact acggctactt cggcaccctc gccggcccgt cgggccgcgg ctactacagc
     1261 ttcgatctcg gcgattggca catcatctcg ctgaacagcg aggtgtcgat gagcaccggc
     1321 tccgcccagg agacgtggtt gcgcgccgat ctcgccgcga gcaccaagca gtgcacgctc
     1381 gcctactggc ataagccgcg cttctccagc ggcacgaacc acggcagcca gagctcggcc
     1441 cagccgttgt ggcaggcgct gtacgatttc catgcggaaa tcgtgctggg gggacacgag
     1501 cacaactacg agcgattcgc cccccagact ccgaccggag cggcggaccc gaccaacggg
     1561 atccgggagt tcgtcgccgg gacgggcggc gagagccatt acaacgacct cggcacccca
     1621 ctggcgaaca gcgaagtgtt caacggtacc acgttcggcg tgctcaagct gacgctcggg
     1681 gcgggcacgt acacctggca gttcatccct gtcgccggac agtcgttcac cgacagcgga
     1741 agcggcacct gccactgatg cgcgcacgcc accgaccggg tcccctccgt ggacggaggg
     1801 acccggcggg tcgttggcgg tagcccgtga agttccaccc gttccggagg gggaccggat
     1861 gcctcacctg cacaaactgt cggtgcgtca ggcgctgctg cgcggcattg ccggactctg
     1921 cgtggctgga tgcacggtgc ccgatcagaa cgtggctggc ccgggcaccg tttcgcagat
     1981 cctcgtcctc cccgagagca ttaccgtcga tcccggtcag gcgctgcaat tccgcgccgt
     2041 cggcctcacg acgaccggtg ccaccgagcc tgtcggagtt cgatggatgg ccaccggcgg
     2101 cggtaaaatc gacgacaacg gcatatacgt cgccgatgac cacccgggcc attttgaagt
     2161 ccgggccatc gtcgatcgcc cgaacctggt cgccggcgcc ggtgtcaaga atcgcggctc
     2221 cctgaaacag gtcgttctca acccgtcgtc ggcgaatgtc ccgccaggcg ggcaggcgca
     2281 ggtcagtgcg tccggcacct tggccaacgg cgacgtcgtt ccggtgaccg tcacgtatgc
     2341 cgccacgtac ggatccgtca cggcctacgg cctctacacc gcgggccaga gcgaagggac
     2401 gtatcagctg atggcgatcg cggggtccgg caacaaggcg ctcgccgata ccgcaacgat
     2461 cgccgtcagc agcaacgcgc cgccaacgct cacccgcgtc gtcctcgagc cgccggaggc
     2521 ggcggttgcc gccggtggga ctcagcagtt cgcggcctac ggtcgcctca gcagcggcga
     2581 tagcgtcgcc gtgagcgtga cctacaccgc aaccggcggc acggtcacgt ccagcgggct
     2641 ctataccgcc ggccagacga ccgggagcta ccgggtggtc gcgacggaga gtggcggggc
     2701 ggtggcggac acctcggcgg tcaccatcgt gaccggcagt ccgacggtca cgtccgtcgt
     2761 gctggtcccc gcgtcggcga cgttgaccgc gggcgggact caacaattaa ccgcctacgc
     2821 ccggctgagc aacggggaca gcggcgcggt gagcgtgacg tacatcgcga gcggcggaac
     2881 catcacgggc gacggactgt acacggcggg ccaaacggcg ggcacctact ccgtcatcgc
     2941 cgtgcaggcc ggcggcacgc tcgcggatac gtcgagcgtc ctgatcacct ccagtccggt
     3001 cgcgtccgtc caggtcacgc cgagcgacgc gacactggcg atcggcacgg gattgcagct
     3061 gcaggtcacc ctcaaggacg cgaatgggac cacactcagc ggtcgcacgg tcgcgtgggc
     3121 gagcagcaac acagcggccg cgacggtcag cgccggaggg gcggtcctgg ccgtcgacgc
     3181 cggggctgcg accgtcacgg cgacgtgcga agggaagagc ggcaccgcca cgatcacggt
     3241 gacggcaccg tcgagcgggt gcaccggctc atggcccgcg gcggtctacc cgcgcatgcc
     3301 gccctccacg gggcaggcct tctatgtgtc ctccgcgtcg ggctccgacg ccaaccccgg
     3361 caccgtcacg gccccctgga ggaccctcca gaaggcgttc gacgccctgc agccggggca
     3421 gatcgcctac ctgcgcgagg ggacgtacgg ggccttttgg tcatcgtccc tgttcagtcg
     3481 ggccggtacc gcctcggcgc ccatcaccgt gcggggctat cccggcgagc gcgcggtcct
     3541 gcacgggcaa atccgcctcg aggggtccta cttccgcctg gcgcatgtcg tcgtggaagg
     3601 tccgagctgc ggcacgtggg gcgcctcgac gcagcaaggc gacaatctgg tgctcatggt
     3661 ccccgggacc acgcaccacg tcgaggtgtc caactcggag gtctatcacg gcggctggca
     3721 tgccggcatc gccgccgggg gcgacgatat ctggatcctc aacaactaca tccacgacaa
     3781 cggcggattc aacgacccga atcagtacaa cacctcgcac gggatctatt accacgacgg
     3841 cacccggggc gtggtcgcca ataacatcct ggagcacaac cgggccaagg ggttgtcggc
     3901 ccgctacagc gccaaccacg tcatcgtggc caacaacacg gtggtgggca acggccggtc
     3961 gggcatggac atcgcggaga acacgcacga ttggctgttc gcgaacaaca tcgtcgccaa
     4021 caacggcaac gtgaatgatg gggtcggcat ccacaccagc ggctcgagcg gcgggacgag
     4081 caatatcgaa atcaacaacg tgctctggaa caacggcgta tccgggacga gtaactggga
     4141 cacggacgcc acgatcatca acaacaaggt ggccgatccg ctcctcgtgt cgcccgtgag
     4201 ctccgttccc tcgacgaccc acgaggggtt tacgaacgac tatcacgtga ggagcggcag
     4261 cccggcgatc gacttcggct ctgcgaccta tgccctgccg ttcgacctcg gcggggtgtg
     4321 ccggccacgc ggtgcgggtc ctgatgcggg ggtttacgaa tactgagaga ggacggcctc
     4381 cggactgtgg cgctcgatcc atgatgtggc gtgcgatcgg cagtcgcacg aaagttccct
     4441 gtgtccttca tcacagccgt ctgatgccgc tgccgctctg gtggaccggc tgtttgccgc
     4501 tctcccgtct tccactagtt tagagcggag caggtgcgtg gtgacgccgc cccggccggc
     4561 gattcctgcg ggatcgccgc tgcggggcgg ttgtgtctcg ggaaggcgtg gcagggtcct
     4621 tcctcatacg aggagacgag atgacactgg
//
LOCUS       DBNGZW010000425         7379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_857690, whole genome shotgun sequence.
ACCESSION   DBNGZW010000425 DBNGZW010000000
VERSION     DBNGZW010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7379
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_857690"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            198..1412
                     /gene="metK"
                     /locus_tag="AC16JC_09990"
     CDS             198..1412
                     /gene="metK"
                     /locus_tag="AC16JC_09990"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR5165064.1"
                     /translation="MPSTRHVFTSESVTEGHPDKIADQISDAVLDAILTNDPAARVAC
                     ETLVTTGMAVVAGEITTSCYVHIPDIVRGMIQSIGYTDANFGFDAQTCAVLTSIDRQS
                     GDIAMGVDTGGAGDQGMMFGYATNETESLMPLPIMLAHRLAEELAAARKRGELPWLRP
                     DGKTQVSVVYEGDRPVGIAKVLVSTQHADKVDNVELRAGVIERVVKPVLSREGFEVAP
                     ADVLVNPTGRFVVGGPQGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSATYA
                     MRWVAKNIVAAGLARRCECQVAYAIGVKEPVSVMVETFGTGTVPDGALEDAVREVFDL
                     TPAGIITALQLRAPIYRPTATYGHFGRPSQTRRVGDGDVQFFGWEQTNRVEDLRLAVQ
                     RSAHYQTAKVKA"
     gene            1409..2752
                     /gene="ahcY"
                     /locus_tag="AC16JC_09995"
     CDS             1409..2752
                     /gene="ahcY"
                     /locus_tag="AC16JC_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015897538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR5165065.1"
                     /translation="MTATAPHAASDVKDLSLADAGRRRTEWAERSMPVLRQLRERFAK
                     DKPLAGRRLSACLHVTTETANLAVTLQAGGADVALCASNPLSTQDDVAAHLVRDHGIK
                     VYAIKGEDHTTYYEHIRAALAHRPDITMDDGADLVGALHMVALNRLDDLAPPVRRWVE
                     TLSAADRAALVRGVIGSTEETTTGVIRLKAMAHDHVLAFPVIAVNDSDTKHMFDNRYG
                     TGQSTLDGILRATNLLIAGSTVVVAGYGWCGRGFAQRARGAGANVLVTEIDPLKALEA
                     QMDGYHVVPMAEAARVGDIFVTLTGNFHVVRLEHMRLMKDGAVIANSGHFNVEIDLEG
                     LAREAQGPTAVRDHVDEWRLGGKRIFVLAEGRLVNLAAAEGHPASVMDMSFANQALAA
                     EYVVQHAAELTRDVHRIPAAIDSEIARLKLAAMGIAIDTLTPEQQRYLASWDQGT"
     gene            2761..3261
                     /locus_tag="AC16JC_10000"
     CDS             2761..3261
                     /locus_tag="AC16JC_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR5165066.1"
                     /translation="MIEVRVAHLGLDKTSNTPVVILQEQNGERVLPIWIGPAEASAIA
                     MELAGMKFARPLTHDLLKQVIVGLGADLRKVIITRVQDNTYYAELHIYRGDAIINIDA
                     RPSDSIAVALRLKAPIFTSEDLLENTAIDTTEHLGEGGPQALDSDALKNYLQNLDPED
                     FGKFTP"
     gene            3263..4276
                     /locus_tag="AC16JC_10005"
     CDS             3263..4276
                     /locus_tag="AC16JC_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165067.1"
                     /translation="MTLGALLALLGAGVTVLASQGGAGGGSVSGVLRRGGPDGAPLAG
                     RWVVLHQVTAGGGGPVDSARTDAGGRWQLRARQVDTMAIYIASALYDGLAYFSPPLRV
                     QSGKAVAAGPLVVYDTTSGGPAIALQRRLVTIAGAGKDGSREVLELLELALEGEKTRV
                     APDTVRPVWTVVIPAAAVQFEVQQGDFSPDAVTQRGDTVLLFAPVQPGRSRQLSFRYV
                     LPASSAGTVVVPIDQPTHELDLLLEDTVAVVRAPGLTGGGVEQIETRRFAGYRADSLA
                     AGAPVSIVFPAKQFRTDRLLPLLVVVIVAVLGVGLWVALRRGEGVGSGEQGTGQSSRT
                     GKK"
     regulatory      4379..4488
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            4598..5446
                     /locus_tag="AC16JC_10010"
     CDS             4598..5446
                     /locus_tag="AC16JC_10010"
                     /inference="COORDINATES: protein motif:HMM:NF013648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5165068.1"
                     /translation="MRRCAQLLVLLALACRPAERGASAGTAPAQRIVSLAPSLTELLF
                     ALGAGPQVVGRTVYCLYPPAARAVPSVGDGLNPNVEAIAARRPDLVLLYRSPQTETAA
                     RQLDALGIPTMLLRDDRMEDVARTARLLGRATGRPAAGDSIARVIDSLLAAPVPPSRA
                     RIALVAWDAPPIVIGSGSYLDELVTMAGAENVFHDLHTPSATVSLETIAARDPDWIAI
                     LRDSLSAEPPAWAGRREWRTVRAVREARFLILPSELFGQPSPRAPAAVAFLASLLNHS
                     PLPTRQ"
     gene            5443..6384
                     /locus_tag="AC16JC_10015"
     CDS             5443..6384
                     /locus_tag="AC16JC_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008743553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR5165069.1"
                     /translation="MKWLVLSLALLAALLAAVTLGPAAVPAGQLFHSAIFWDLRLPRA
                     LLALLVGGSLGAAGASLQSLVRNPLADPFLLGLSGGAGLGAVLAIALHLGGAWTLPLA
                     AFAGALLALGLVFRLGLVGGSALEPRVLLLGGVAVGAFCGAVTTAVVSLAEASALRNA
                     YLWLWGGLSGASWDTVTLVAVYVPLPLVVLLAAARPLDLLTLGEEPARYLGADVAVTR
                     RRVYLAASLLTAAAVAVAGVIGFVGLIVPHLGRALFGGRHRALLPASFLAGGILLLLA
                     DTLARTAVAPRELPVGVVTALVGVPLFVLLLRRWTAA"
     gene            6369..7163
                     /locus_tag="AC16JC_10020"
     CDS             6369..7163
                     /locus_tag="AC16JC_10020"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5165070.1"
                     /translation="MDRGVILDVRDASFRYPGAARDALAGVSLALSAGEFVALLGPNG
                     SGKTTLVRLALGLHGPRVGSAAILGRPAGQWPRGALARVVGVVSQREDNLFPQRVRET
                     VRLGRYPHLGPWAREREEDRAAVERALERCDVARLADRWLWTLSGGEYQRVRIARALA
                     QEPQLLVLDEPTASLDLRHEMELFELVRDLVDRSGLAAVLVTHDVNLAARFADRLVLL
                     AEGSVRAAGTPADVLRRDVVEAVFGWPLAVSSFDDRPQLTPLRRQP"
     gene            7180..>7379
                     /locus_tag="AC16JC_10025"
     CDS             7180..>7379
                     /locus_tag="AC16JC_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165071.1"
                     /translation="MRYGFARRLCLTVSCSTQFAFAVAAQQPDTVTLPPVVVTANRIP
                     TTLNAVSSFDDRPQLTPLRRQP"
BASE COUNT         1012 a         2581 c         2628 g         1158 t
ORIGIN      
        1 agctcagccg tgggagagcc gccgcggccg cggccgccgc actcgaagcc cgcaacgagg
       61 tcgaggtgct ggagatcctg cgggtgggtg tcgccagctg cggtgtggat ccgttgctgc
      121 tctgatatgc acggcccggt tgcccgacgg tcgggcccgg gctacacttg aaccctcgac
      181 gctcccccaa ggcaaagatg ccctccactc gacacgtctt cacatccgaa tccgtcaccg
      241 aaggccatcc ggacaagatc gccgatcaga tctccgacgc ggtcctggat gccatcctga
      301 ccaacgatcc cgcggcccgc gtggcgtgtg aaaccctcgt gaccacggga atggccgtgg
      361 tcgcgggaga gatcacgacg agctgttacg tccacatccc cgacatcgtt cgcggtatga
      421 tccagtcgat cggctacacc gacgcgaact tcggcttcga cgcccagacc tgcgcggtgc
      481 tgacgagcat cgatcgccag tccggcgaca tcgcgatggg cgtggacacg ggcggagcgg
      541 gcgatcaggg gatgatgttc ggctacgcga ccaacgaaac cgagtcgctc atgccgctcc
      601 ccatcatgct ggcgcaccgg ctggccgagg agctggcggc ggcgcgcaag cgcggagagc
      661 tgccgtggct ccgtcccgac ggcaagaccc aggtcagcgt ggtctacgag ggcgaccgcc
      721 cggtcggcat cgccaaggtc ctggtctcca cccagcacgc cgacaaggtg gacaacgtcg
      781 agctgcgcgc cggggtgatc gagcgggtgg tgaagccggt gctctcgcgc gagggattcg
      841 aggtcgcccc cgcggacgtg ctggtcaatc cgaccggccg cttcgtggtc ggcgggccgc
      901 agggcgacgc ggggctcacg ggccggaaga tcatcgtgga cacctacggc ggcatggcgc
      961 gccacggcgg cggagcgttc agcggcaagg acccttccaa ggtggaccgc tcggcgacct
     1021 acgcgatgcg ctgggtcgcg aagaacatcg tggccgcggg gctcgcccgc cgctgcgagt
     1081 gccaggtggc ctacgctatc ggcgtgaagg agccggtgtc ggtgatggtc gagacgttcg
     1141 gcaccggcac cgtccccgac ggggcgttgg aggacgcggt gcgcgaggtg ttcgacctca
     1201 cgcccgccgg catcatcacg gcgctgcagc tgcgcgcgcc gatctaccgt cccacggcga
     1261 cctacggcca cttcggtcgg ccgtcgcaga cccgccgcgt gggtgacggg gatgtgcagt
     1321 tcttcgggtg ggagcagacc aaccgcgtgg aagacctgcg gctcgccgtg cagcgatccg
     1381 ctcactacca gaccgccaag gtgaaggcat gaccgcgacc gctccgcacg ccgcttccga
     1441 tgtgaaggat ctctcgctgg cggacgccgg ccggcgccgg accgagtggg ccgagcgctc
     1501 catgccggtg ttgcgccagc tgcgcgagcg gtttgcgaag gacaaacccc tcgccgggcg
     1561 gcggctctcg gcgtgcctcc acgtcacgac ggagaccgcc aacctcgccg tcacgctcca
     1621 ggcgggcggt gccgacgtgg cgttgtgcgc ctccaacccg ctgtccacgc aggacgacgt
     1681 cgcggcccac ctggtgcgcg accacgggat caaggtctac gccatcaagg gcgaggacca
     1741 cacgacctac tacgagcaca ttcgcgccgc gctcgcgcac cggcccgaca tcaccatgga
     1801 tgacggcgcc gacctggtcg gcgcgttgca catggtggcg ctcaaccggc tcgacgacct
     1861 ggcacctccg gtgcgccggt gggtggaaac gctgagtgcg gcggaccggg ccgcgctggt
     1921 gcgcggggtg atcggctcca ccgaagagac caccaccggg gtgatccggc tcaaggcgat
     1981 ggcgcacgac catgtgctcg ccttccccgt gatcgcggtc aacgactccg acaccaagca
     2041 catgttcgac aaccgctatg gcaccggcca gtcgacgctc gacgggatcc tgcgcgccac
     2101 caacctcctc atcgcgggga gcacggtggt ggtggcgggg tacggatggt gcggacgtgg
     2161 cttcgctcag cgggcacgcg gggcgggggc gaacgtcttg gtgaccgaga tcgacccgct
     2221 gaaggcgctc gaggcccaga tggacggcta ccacgtcgtg ccgatggccg aagcggcgcg
     2281 ggtgggggac atcttcgtca cgctcacggg gaatttccac gtggtgcggc tggagcatat
     2341 gcgcctgatg aaggacggcg cggtgatcgc gaattcgggg cacttcaacg tcgagatcga
     2401 cctcgagggg ctggcccgcg aggcccaggg gccgaccgcg gtgcgcgacc acgtggacga
     2461 gtggcggctc ggcgggaagc ggatcttcgt gctggccgag ggacggctcg tgaatctggc
     2521 ggccgcggaa gggcatcccg ccagtgtcat ggacatgtcg ttcgccaatc aggcgctcgc
     2581 ggccgagtac gtggtgcagc acgctgcgga gctgaccagg gacgtgcacc gcatccccgc
     2641 cgcgatcgac agcgaaatcg ctcgcctgaa gctcgccgcc atggggatcg cgatcgacac
     2701 gctgacgccc gagcagcagc gctacctggc ctcctgggac caggggacct gaggaggctc
     2761 gtgatcgaag tgcgcgtcgc ccacctcggg ctcgacaaga cgagcaatac gccggtcgtc
     2821 atcctgcagg agcagaatgg cgagcgggtg ctgccgatct ggatcgggcc ggccgaggcg
     2881 agcgcgatcg cgatggagct ggcgggaatg aagttcgcac ggccgctcac ccacgatctg
     2941 ctcaaacagg tgatcgtggg cctgggagcg gacctgcgca aggtgatcat cacccgggtc
     3001 caggacaaca cctactacgc cgagctgcac atctatcgcg gcgacgcgat catcaacatc
     3061 gatgctcgcc cctcggactc gatcgcggtg gcgctgcgcc tcaaggcgcc gatcttcacg
     3121 agcgaagatc tgctcgagaa caccgccatc gacaccaccg agcacctggg agagggcggc
     3181 ccccaggccc tcgactccga cgcgctcaag aactacctcc agaatctcga tcctgaggat
     3241 ttcgggaagt tcacgccgta gggtgacgct cggcgcgctc ctcgcgctgc tcggcgccgg
     3301 cgtgacggtc ctcgcgtccc aggggggggc ggggggcggc tccgtgtccg gggtgctgcg
     3361 gcgtggtggg ccggacggcg ccccgctcgc cggccgctgg gtggtgctgc accaggtcac
     3421 ggccggcggc ggcgggcccg tggattcggc ccggaccgac gcgggcgggc gctggcagct
     3481 gcgggcgcgc caggtggaca cgatggccat ctatatcgcc agcgcgctgt acgacggact
     3541 cgcgtacttc tccccaccgt tgcgcgtcca gagcggtaag gccgtggcag ccggtcccct
     3601 cgtcgtctac gacacgacct cgggcggccc cgcgatcgcc ctgcagcgcc gcctcgtcac
     3661 gatcgccggg gcgggcaagg acggctcgcg cgaagtgctc gagctgctcg agctggcgct
     3721 cgagggggag aagacgcgcg tcgcgccgga taccgtgcgg cccgtctgga cggtcgtgat
     3781 tcccgccgcc gccgtgcagt tcgaggtgca gcagggcgat ttctctcccg atgcggtgac
     3841 ccagcgcggc gacaccgtgc tgctcttcgc gccggtgcag ccggggcgca gccgccagct
     3901 gtccttccgc tacgtgctcc cggcgtcgtc ggcgggcacg gtcgtggtgc cgatcgacca
     3961 gcccacacac gagctcgatc tgctgctcga ggacacggtg gccgtcgtga gggccccggg
     4021 cctcaccggc ggcggtgtgg aacagatcga aacgcgtcgc ttcgccggat atcgcgccga
     4081 ctcgcttgcg gccggcgcgc ccgtctcgat cgtgtttccg gcgaaacagt tccgcaccga
     4141 ccggctgctg ccgctcctcg tcgtcgtgat cgtcgcggtc ctgggcgtcg gattgtgggt
     4201 ggcgctgaga agaggcgagg gagtagggag cggggagcag ggaacgggac agagcagccg
     4261 caccgggaag aaatgacacg tctcggacca ctccccgctc cctactcccc gctcccttgc
     4321 acgagctcca ccgctgctgt agagtgagtc cgtagtccac atcgcgaacg gggatcatgg
     4381 aagccggtgt gagtccggcg cggccccgcc actgtaacgg gcatggaatg ccttgcgtca
     4441 ctgggaaacc gggaagacgc gtggtgcagt agcccgaagc caggagacct cgccgttcgc
     4501 gccacctgca gtccctcgcg ggagggatgg tggcgtcaca gcgcagttcg gcgctgtcgc
     4561 tcgcccatct ccgctccgcc gggggtgggt gttttgcatg cggcgctgcg cacagctgct
     4621 cgtccttctg gccctggcct gccgtcccgc tgagcggggg gcgagcgcgg gcacggcgcc
     4681 cgcccaacgc atcgtctcgc tcgccccatc cctcaccgag ctgttgttcg ctttgggcgc
     4741 gggcccccag gtcgtggggc gtaccgtgta ctgcctctac ccgcccgcgg cgcgtgccgt
     4801 gccgagtgtg ggggacgggt tgaatccgaa cgtcgaggcc atcgccgcac ggcgcccgga
     4861 tctggtcctg ctgtatcgct cgccccagac cgagacggcc gcgcgtcagc tcgatgccct
     4921 ggggatcccc acgatgctgc tgcgtgacga ccggatggaa gacgtggctc gcaccgcgcg
     4981 cctgctggga cgcgcgaccg gccggccggc cgcgggggac agcatcgccc gggtgatcga
     5041 ctcgctcctg gccgcgcccg ttccaccatc ccgcgcgcgc atcgcgctcg tcgcatggga
     5101 tgcgcccccg atcgtgatcg ggagcgggag ctacctggac gagctggtga ccatggcggg
     5161 cgccgagaat gtgtttcacg acctgcacac gccgtcggcc accgtctccc tcgagaccat
     5221 cgcggcacgc gaccctgact ggatcgcgat tctgcgcgac agcctgagcg ctgagcctcc
     5281 cgcgtgggcc gggcggcgcg agtggcgcac ggtccgggcc gtacgcgaag cacgatttct
     5341 catactgcca tcggagttgt ttggacaacc gtcaccccgg gcaccggcag ccgtcgcctt
     5401 cctggcaagc ctcctgaacc actccccact ccccactcgc cagtgaagtg gctcgtcctc
     5461 tccctcgccc ttctggccgc gttgctcgcg gccgtcaccc tcggaccggc cgccgtgccg
     5521 gccgggcagc tgttccactc ggcgatcttc tgggatctgc ggctgccgcg cgcgctgctc
     5581 gcgctcctgg tgggcgggtc cctgggagcg gccggggcga gtctgcagtc cctggtccgc
     5641 aatcccctcg ccgatccgtt cctgctcggg ctctcgggcg gcgccggact gggcgccgtc
     5701 ctggccatcg cgttgcacct cggcggggcg tggaccctgc cgctggccgc attcgcgggc
     5761 gcgctgctgg ccctcggact ggtgtttcgc ctcggactcg tcggcgggtc ggcgctcgaa
     5821 ccccgcgtac tgctgctcgg cggtgtggcg gtcggcgcct tctgcggcgc ggtcaccacg
     5881 gcggtggtat ccctggccga ggcgagcgcg ctgcgcaacg cctacctgtg gctgtggggc
     5941 gggttgtcgg gcgcgtcgtg ggacaccgtg actctcgtgg cggtgtacgt gccgcttccg
     6001 ctcgttgtac tgctggccgc ggcacggccg ctcgatctcc tcacgctcgg cgaggagccg
     6061 gcgcgctacc tcggggccga cgtcgcggtg acccggcgcc gggtctatct cgccgcgtcg
     6121 ctgctcacgg cggcggcggt ggcggtcgcg ggagtcatcg gattcgtggg gctcatcgtg
     6181 ccgcatctgg ggcgcgcgct gttcggtggg cgccaccgcg cgctgctgcc cgcgtcgttc
     6241 ctcgcaggcg gcatcctgct cctgcttgcc gacaccctgg cgcgcacggc ggtcgctccg
     6301 cgggagctgc cggtgggcgt cgtcacggcg ctggtgggcg tcccgctctt cgtcctgctg
     6361 ctccggcgat ggaccgcggc gtgatcctcg acgtgcgcga cgcctcattc cgctaccccg
     6421 gagcggcacg cgacgccctc gcgggggttt cgctcgcgct gtccgcgggc gagttcgtgg
     6481 ccctgctggg gcccaacggc agcggcaaga cgacgctggt gcggctcgcg ctgggcctcc
     6541 atgggccgcg cgtgggctcc gcggccatcc tgggacgccc ggcggggcag tggccacgcg
     6601 gcgcgctcgc ccgggtggtg ggcgtcgtat cccagcgcga ggacaacctg tttccccagc
     6661 gcgtgcgcga gacggtacgg ctgggccggt acccccacct cggcccctgg gcgcgggagc
     6721 gcgaggagga ccgggcagcg gtggagcggg cactggagcg ctgcgatgtg gcccgtctgg
     6781 ccgaccgctg gctctggacc ctctcgggtg gcgagtacca gcgggtacgg atcgcgcgag
     6841 ccctggcgca ggagccgcag ctgctcgtgc tcgacgagcc gaccgcatcg ctcgacctgc
     6901 gtcacgaaat ggagctgttc gagctggtcc gcgatctggt ggaccggtcg gggctggccg
     6961 cggtgctcgt cacgcacgac gtcaacctcg ccgcccggtt cgccgaccgg ctggtgctcc
     7021 tggccgaagg ctccgtgcgg gccgccggta cgccggcgga cgtgctgcgt cgcgacgtcg
     7081 tggaggcggt gttcggctgg cccctcgccg tgtcttcgtt cgatgatcgc ccccaactga
     7141 ccccgttgcg ccgtcaaccc tgaacgcgag aacgccctca tgcgctacgg tttcgctcga
     7201 cgcctttgct tgacggtctc ctgctcgacg cagttcgcct ttgcggtggc ggcccagcag
     7261 cccgacaccg tcactctccc tcccgtcgtc gtgacggcga atcgtatccc gacgaccctg
     7321 aacgccgttt cgtcgttcga tgatcgtccg caattgactc cgttgcgccg tcaaccctg
//
LOCUS       DBNGZW010000426         3661 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_244345, whole genome shotgun sequence.
ACCESSION   DBNGZW010000426 DBNGZW010000000
VERSION     DBNGZW010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_244345"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="AC16JC_10030"
     CDS             <1..733
                     /locus_tag="AC16JC_10030"
                     /inference="COORDINATES: protein motif:HMM:NF012356.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR5165072.1"
                     /translation="DSLIAAVRSGSAAGLLSGFLRLQDTLPAYRWSPLLSNHDGTRSM
                     TLLGGDVSRARLAATLLLTLPGLPFVYYGEEIGMTGDKPDPRLRTPMQWAPVRGLGFT
                     TGTPWEAAQSDSMTTSVALQDSDPGSLLNLYRRLIHLRKENEALATGRLVPLVASSTQ
                     VAAYLRRAGDHAVLVVANLGGTAASVSVTSGDSVLPPGAFGPKDLLGGADGARLQVGR
                     DGRIRGYVLAEVGPRQALVFELDPR"
     gene            complement(800..2077)
                     /locus_tag="AC16JC_10035"
     CDS             complement(800..2077)
                     /locus_tag="AC16JC_10035"
                     /inference="COORDINATES: protein motif:HMM:NF012356.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR5165073.1"
                     /translation="MRVTAPDTSWVARSTIYEVNVRDFSPSGDLRGVTRGLDRIGAVG
                     ANVVWLMPIYPVGILNAKGPLGSPYAVRDYLAIDSAFGTAADLRELVAAAHARGMKVL
                     LDWVPDHTAWDNVWMRQHPDYYIRDERGQPIVPRDKQGKPTDWTDVAQLDYGNPELRR
                     AMIAAMRYWLEQFDLDGFRVDVAGFVPDAFWREAVPALRAAAPRPILLLAEWGDLGMH
                     RLGFDLTYGWDSYSRLKAVWRGAPADAFVRSELADLEAMPPGGMRLRFTTNHDETAWD
                     KPPVTLFDGAAGARAAFVAMALLPGRPLIYDGQEVESPQQLGLFRRDSIVWAQHDTIA
                     ARSFYRRVLDLAQTDSAFLTGPFEQVGTSAEKDVIAYRRGAALVLANARSRPVRVVVT
                     GFAVDGARDLLSDRTQAGDTVSLQAYGAMVLRP"
     gene            2234..>3661
                     /locus_tag="AC16JC_10040"
     CDS             2234..>3661
                     /locus_tag="AC16JC_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013763350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR5165074.1"
                     /translation="MSRALPVLLLFSFTSIPSNGAAQGEGTRLLRQPTISERQVAFTY
                     GADIWVADRSGGAARRLTSTPAVEANPHFSPEGKFIAFTSNRSGTMQVYVVPVEGGEP
                     TRLTWYPAPSFARGWTPDGSRVLYASLRETAPVGYARLWTVSRTGGPSTLLPAPWGND
                     GSYAPDGRRIVVDRVRRWDVEWRGYRGGQNTPLTILNLGTLEETRLPNERTQDIAPVW
                     LGDKVYFISDRDWASNVWSYDVASGALQQLTRFTGAEVKSLSGGAGVLVFEQDGWIHT
                     LDPASGRTQRLDIAAQGDFPWMEPRWEDVSDRVAAASLSPKGKRILLEARGEIFTVPV
                     ENGSPRDLTRSPDAADRAPVWSPDGRRIAWFRDAGEGYALMVADQDGLGTPRSIAIGE
                     SRMAWEPAWSPDGKQIAFEDNKVRIRVVDVESGRIRTVDAGGANIERGGMELAWSPDS
                     RYLAYAKTYPNMFRRIVVWSRDSGSV"
BASE COUNT          541 a         1281 c         1319 g          520 t
ORIGIN      
        1 cgactcgctg attgcggcgg tgcggagcgg ctcggccgcc gggctcctgt cgggattcct
       61 gcggctgcag gatacgctcc cggcgtaccg ctggtcgcca ttactcagca accacgacgg
      121 cacccgctcc atgaccctgc tcggcggcga cgtttcccgg gcgcggctgg cggccaccct
      181 gctgctcacc ctccccgggc ttcccttcgt ctattacggt gaggaaatcg ggatgacggg
      241 ggacaagccc gacccgcgcc tgcggacccc gatgcagtgg gccccggtgc gcgggctggg
      301 cttcaccacc ggcacgccct gggaggcggc gcagtcggat tcgatgacga ccagcgttgc
      361 gctgcaggac tccgatccgg gatcgctgct caacctgtac cggcgcctga tccacctgcg
      421 caaggagaac gaggcgctcg cgaccggccg gctcgtgccg ctcgtcgctt cgagcacgca
      481 ggtggccgct tacctgcgac gtgcgggtga ccacgccgtg ctcgtggtgg cgaacctggg
      541 ggggaccgcc gccagcgtat cggtcacctc gggggatagc gtgctcccac cgggagcatt
      601 cggaccgaag gacctgctcg gaggtgcgga cggcgccaga ctgcaggtgg ggcgtgacgg
      661 gcgaatccga ggatacgtcc tcgccgaggt cgggccccgt caggcgctcg tgttcgagct
      721 cgatccgcgc taggagcgcc agcgggggat gtcattccga gcccggcgca gcggggcgag
      781 gaatccctcc gtccagggtc tagggcctga gcaccatcgc cccgtacgcc tgaagcgaga
      841 ccgtatcacc ggcctgggta cggtcggaca gtagatcgcg tgcgccgtcc accgcgaacc
      901 ccgtgacgac cactcgcacc gggcgggacc gggcgttcgc gagcacgagc gcggcaccgc
      961 ggcggtaggc aatcacatcc ttttcggcgc tggtcccgac ctgctcgaag ggtccagtga
     1021 gaaacgccga gtcggtctgc gccaagtcga ggacgcgccg gtagaaggaa cgggccgcga
     1081 tcgtgtcgtg ctgggcccac acgatcgagt cgcgtctgaa gaggcccagc tgctgcgggc
     1141 tctcgacctc ctgcccgtca tagatcagcg gccggcccgg cagcagcgcc atcgcgacga
     1201 aggccgcgcg tgctcccgcc gcgccgtcga acaaggtcac cggcggcttg tcccacgcgg
     1261 tctcgtcgtg gttcgtggtg aagcgcagcc gcatgccgcc cggcggcatg gcctcgaggt
     1321 cggccagctc gctccgcacg aacgcatcgg ccggggcgcc gcgccacacc gccttgagcc
     1381 ggctgtagga atcccagccg taggtgaggt cgaagccgag ccggtgcatc ccgagatcgc
     1441 cccattcggc gagcagcagg atcggccgcg gcgccgcagc gcgcagcgcc ggcacggcct
     1501 cgcgccagaa ggcatccggc acgaagcccg cgacgtccac gcggaagccg tcgaggtcga
     1561 actgctcgag ccagtagcgc atcgcggcga tcatggcgcg gcgcagttcg ggattgccgt
     1621 aatcgagctg cgccacgtcc gtccagtcgg tcggcttgcc ctgcttgtcg cgcggcacga
     1681 tcggctggcc gcgctcgtcg cggatgtagt agtcggggtg ctgccgcatc cagacgttgt
     1741 cccaggcagt gtggtccggc acccagtcga ggaggacctt catgccgcgc gcatgcgccg
     1801 ccgcgacgag ctcccgcaag tccgccgcgg tgccgaaggc ggaatcgatc gcgaggtagt
     1861 cgcgcacggc gtagggggag ccgagcgggc ctttcgcgtt cagaatcccg accggataga
     1921 tcggcatcag ccagaccacg ttcgccccga ccgccccaat gcgatcgagc ccgcgcgtca
     1981 cgccccgcag atcgccgctg ggggagaagt cgcggacgtt gacctcgtag atggtgctgc
     2041 gcgcgaccca ggacgtgtcg ggcgccgtga cgcgcaccgg cgccggctgg ctcggagagc
     2101 aacccgctgc cagcgggaga aggaccgcga tcgcggcaac gcagtttctc gtccgcacga
     2161 ggccaatgta gcggctcctt gcgtcctccg ccgggcggcg gaagcttggg agacctgctc
     2221 tcggagtcat gccttgtccc gtgcactgcc cgtcctcctc ctcttctcct tcacttccat
     2281 cccgagcaac ggcgcggcgc agggagaggg gacgcgtctg ctccggcagc ccaccatcag
     2341 cgagcgtcag gtcgcgttca cgtacggcgc ggacatctgg gtggcggacc gcagcggagg
     2401 cgcggcccgg cgcctgacga gcaccccggc cgtcgaagcg aacccgcact tctcgcccga
     2461 aggcaaattc atcgcgttca cgtccaaccg gtccggcacg atgcaggtgt acgtggtgcc
     2521 ggtggagggc ggcgagccca cgcggctcac ctggtatccg gcgccgtcgt tcgcgcgcgg
     2581 ctggacgccc gacggcagcc gcgtgctcta tgcgtcgctg cgggagacgg cgccggtggg
     2641 atacgcgcgg ctctggaccg tgtcgcgcac gggcggcccg tccaccctgc tgccggcgcc
     2701 ctggggcaac gacggctcgt acgcgccgga cgggcgccgc atcgtcgtgg accgggtgcg
     2761 ccgctgggac gtggagtggc gcggctaccg tggagggcag aacacgccgc tcacgattct
     2821 caacctcggc acgctcgagg agacgcggct gcccaacgag cgcacccagg acatcgcgcc
     2881 ggtgtggctc ggcgacaagg tctacttcat ctccgaccgc gactgggcgt cgaacgtgtg
     2941 gtcctacgac gtggcgagcg gcgccctcca gcagctgacc aggttcaccg gcgccgaggt
     3001 gaagtcgctc tcgggcggcg cgggcgtgct ggtgttcgag caggacggct ggatccacac
     3061 gctcgacccc gccagcgggc gcacccagcg gctcgacatc gccgcgcagg gcgacttccc
     3121 gtggatggag ccgcgctggg aagacgtgag cgaccgcgtg gcggcggcgt cgctctcacc
     3181 caaggggaag cgcatcctcc tcgaggcgcg gggggagatc ttcaccgtgc cggtggagaa
     3241 cggcagccca cgcgacctca cgcgctcgcc cgatgccgcc gaccgcgcgc cggtctggtc
     3301 ccccgacggg cggcgcatcg cctggttcag ggacgcgggc gagggctacg cgctgatggt
     3361 ggccgaccag gatgggctgg gcaccccgcg cagcatcgcg atcggcgagt cgcggatggc
     3421 gtgggagccg gcgtggtcgc ccgacggcaa gcagatcgcc ttcgaggaca acaaggtgag
     3481 gattcgggtg gtggacgtgg agtcgggccg gatccgcacg gtggatgcgg gcggcgccaa
     3541 catcgagcgc ggcgggatgg agctcgcctg gtcgcccgac tcccgctacc tcgcctacgc
     3601 caagacctat cccaacatgt tccgccgcat cgtggtctgg tcgcgggaca gcggcagcgt
     3661 c
//
LOCUS       DBNGZW010000427         6726 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_176949, whole genome shotgun sequence.
ACCESSION   DBNGZW010000427 DBNGZW010000000
VERSION     DBNGZW010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6726)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6726)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6726
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_176949"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="AC16JC_10045"
     CDS             <1..400
                     /locus_tag="AC16JC_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR5165075.1"
                     /translation="VTGEMTAQLWAASDRLDTDWTVMLLDVWPNGHAERVQDGLVRAR
                     FRRGMDREVPLVPGRVERYDIDLWFTSMVFQPGHRLRVSIASALFPKYDRNLNTGGNN
                     ERDSTFVVAHQRVLHDAAHPSHVVLPVIPR"
     gene            complement(486..902)
                     /locus_tag="AC16JC_10050"
     CDS             complement(486..902)
                     /locus_tag="AC16JC_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5165076.1"
                     /translation="MSSSTTSSDRNLASISPFFIVQDLQASISHYIERFGFTLDFQGP
                     ADDVYYGHVSRDGIGIMLKAIRPDVRPCPNHTRHEWARWDAYIYTRDPDVLFDEFKQR
                     GASFVKELSLIDDGLWGFEVTDADGYVLAFFRLRKD"
     gene            complement(1048..1869)
                     /locus_tag="AC16JC_10055"
     CDS             complement(1048..1869)
                     /locus_tag="AC16JC_10055"
                     /inference="COORDINATES: protein motif:HMM:NF012632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0005085 - guanyl-nucleotide exchange
                     factor activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RCC1 domain-containing protein"
                     /protein_id="HIR5165077.1"
                     /translation="MARTASSSSMRRSNRVRGTRRGWTSPRDIVFQQLSAGHGMTCGV
                     TTANAAYCWGDNGLGQLGDSSTQQRNAPTLVAGGYAFTQISAGETHTCAVTTGHDAYC
                     WGNNTTGQLGHGPGDHAFVPALVSGGLSFAQVSVGKEHSCGVTVGDAAYCWGANTDGQ
                     LGDSTTDVSTIPAAVSGGFSFSQLDAGDLHTCGVTTGNDAYCWGYNSAGEIGHGSSGG
                     PPVLQPLLVVGGLQLAHVSGGYAHTCAIAVDYPRGYCWGLDNGVIGTGSPVPIAQ"
     gene            complement(1787..2965)
                     /locus_tag="AC16JC_10060"
     CDS             complement(1787..2965)
                     /locus_tag="AC16JC_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5165078.1"
                     /translation="MVEATVESGPARPLRLGMVGGGRGAFIGGVHRLCARMDGQYELV
                     AGALSSDPVRARESGADLQLAPTRVYTSFVEMAAAEAQRPDGIEVVSIVTPNDSHHAI
                     ARAFLDRGIDVICDKPMTTTVEDALDLVQAVRRTGLIFGVTHNYTGYPMVRQAREMIA
                     AGELGTIRVVQVEYVQDWLTTRLEDTGQKQAAWRVDPARAGAGGCLGDIGTHAYNLAG
                     FVTGLAARELAADLSRFVPGRKLDDNVHILLRFENGARGMLWSSQVAPGNENGLRVRV
                     YGDQAGLEWQQEHPNQLRFAPVGQAPRVLSRGGPNLGSAAALATRIPSGHPEGYLEAF
                     ANLYRDLAEQIRARRAMRKPDRAALLVPTVEDGADGVKFIHAAVESSARNSAWVDVTP
                     "
     gene            complement(2967..4022)
                     /locus_tag="AC16JC_10065"
     CDS             complement(2967..4022)
                     /locus_tag="AC16JC_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007828672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5165079.1"
                     /translation="MRTIKGPAIFLAQFAGDAPPFNTLKAIAGWAAGLGFKGVQIPSW
                     DARLFDLAKAAESQTYCDEVKGILAERGLQVTELATHLQGQLVAVHPAFDTAFDGFAA
                     PEVRGKPAARTAWAVEQMKLAARASRRLGVTEHVTFSGALAWPYIYPWPQRPAGLIET
                     AFDELARRWRPILDVFDENGVNACFEIHPGEDLHDGVTFEMFLERLDGHKRCNLLYDP
                     SHFVLQQLDYLEYIDLYHDRIRMFHVKDAEFNPTGRQGVYGGYQPWINRAGRFRSPGD
                     GQVNFKAIFSKLAQYDYAGWAVLEWECCLKHPEDGAREGAKLIADHIIRVTEKAFDDF
                     SGAHTDEAANRRMLGVA"
     gene            complement(4093..4530)
                     /locus_tag="AC16JC_10070"
     CDS             complement(4093..4530)
                     /locus_tag="AC16JC_10070"
                     /inference="COORDINATES: protein motif:HMM:NF022588.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HIR5165080.1"
                     /translation="MGAARVTVDYSRPARRGRTLVGDLIPYDRVWRTGADAATQLTTS
                     APIELAGVPLHPGTYTLWTLPTRSGGGVSLIINGQSGQWGTEYHADRDVARRPMTVDS
                     LPGNVERFTIRIEPAGRLVMEWGAFRWSAPIEAGRSAPRRPRA"
     gene            complement(4512..5291)
                     /locus_tag="AC16JC_10075"
     CDS             complement(4512..5291)
                     /locus_tag="AC16JC_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165081.1"
                     /translation="MSTPSVQARLALLLVLGGCTGPETYGFVAVLGNDTTSVERVTRT
                     GHRIIGDAIGRSPTVTRRHWEAELGPNGVIAHWSMDTDIPNGPPAGRHLHHTATFTDK
                     VTAFTRGTSAGMRSFAYQKRYAETVPWNAFVYATWELLLDAARRQPDSTRARIGQYFF
                     EGWDEGHVGYADIERNPDGSYAIRSTGLAGTGTARLDVHGRLAAYSGQGTTYKQEVRR
                     ITDVPDIDAIAARFAADEKRTGPATSLSRATPSAPRWGPPV"
     gene            complement(5350..6474)
                     /locus_tag="AC16JC_10080"
     CDS             complement(5350..6474)
                     /locus_tag="AC16JC_10080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165082.1"
                     /translation="MSDGPAGRGWDWLVQEARDACFTLIGEEHGVAETAQLAAALFNA
                     LRGSGYSRMAIELSPIIAQDIEAAARRNGLQGIVDFFAAPDTWSPMYLREEAQFLAAV
                     INAAPRSERVLWGLDREIFSDRYLISKLEARVPRPARESFARLKEASTNAWARHQQNP
                     SPDNLFLFSQDPAVVSAVRAAWPNPDRESDTILRTLEESLAINAVARTGSPWASSQRR
                     AQWTRSNLAARLREERGRGSAPKVMLKFGYNHMIRGANYVNIFDVGAMTDEVAAMSGG
                     GAFHILVLPGPGSRQAVPGPGRSFVSVSSDEFDEFRSGDQRLTRVLPNANATGHEVID
                     LRALRPLAMRGLEGWNADVVRTIHGYDAAVIWKGAHASSS"
BASE COUNT         1113 a         2230 c         2227 g         1156 t
ORIGIN      
        1 ggtcacgggc gagatgacgg cgcagctgtg ggcggccagc gaccggctgg acaccgactg
       61 gacggtcatg ctgctggacg tgtggccgaa cgggcacgcc gagcgggtgc aggacgggct
      121 ggtgcgggcg cgcttccggc gggggatgga tcgggaagtg ccgctggtgc cggggcgggt
      181 cgagcgctac gacatcgacc tgtggttcac ttcgatggtg ttccagccgg ggcaccggct
      241 gcgggtcagc attgcctcgg cgctgttccc caagtacgac cgcaacctga acaccggcgg
      301 gaacaacgag cgggattcga cgtttgtggt ggcgcaccag cgggtgctgc acgacgcggc
      361 gcacccgtcc cacgtggtgc tcccggtgat tccgcgctag tcctcatccc tgcccccgct
      421 cccttcgggt cctcaccccc tgtccccctc tcccttcggg agagggggaa ctacgctcga
      481 aagcttcagt ctttgcggag ccgaaagaag gccaggacgt acccgtcggc atcggtgacc
      541 tcgaagcccc acagcccgtc gtcgataagg gacagctcct tcacgaacga cgccccccgc
      601 tgcttgaact cgtcgaacag gacgtcgggg tcgcgggtgt agatgtaggc atcccaccga
      661 gcccattcgt ggcgggtgtg gttgggacag ggacgcacgt cgggccggat cgccttgagc
      721 atgatgccga tcccgtcccg actcacgtgg ccgtagtaga cgtcgtcggc cggtccctgg
      781 aagtccagcg tgaagccgaa gcgctcgatg tagtgcgaga tcgaggcctg caggtcctgc
      841 acgatgaaga acgggctgat cgaagcgaga tttctgtccg atgacgtggt gctcgagctc
      901 atgtaccaat cccctggcgg cgacggatag gcgcgctcca ttggaaggag ccccacccct
      961 gatgtattgg cttgcgatca agtaggcgcg gcataccatc tactcctgca atacaccctc
     1021 gtcaaatttc ggctttcgag gccgcaccta ctgcgcgatc ggcaccgggg accccgtccc
     1081 gatgacaccg ttatccaggc cccagcagta gccccgggga tagtccacgg caatggcgca
     1141 ggtgtgcgcg tacccgccac tcacgtgtgc caactgcaat ccgccgacga cgagcaatgg
     1201 ttgcagtacg ggtgggccgc cggacgatcc atggccgatt tcacccgcgc tgttgtagcc
     1261 ccagcagtac gcgtcgttac ccgtcgtgac cccgcacgtg tgaagatcgc ccgcgtcgag
     1321 ctgtgagaac gagaagccgc cgctgacggc tgcggggatc gtactgacat cggtggtgct
     1381 gtccccaagc tgaccatcgg tgttggcgcc ccagcagtac gcggcgtcgc cgacggtgac
     1441 tccgcagctg tgttccttgc cgacgctcac ctgagcgaaa ctcaacccgc cggagacgag
     1501 ggcgggcacg aatgcgtgat ccccggggcc gtgccccagc tgaccggtcg tgttgttgcc
     1561 ccagcaatag gcatcatgtc ccgtggtcac cgcacaggtg tgggtttcgc cggcactgat
     1621 ttgtgtgaac gcgtacccgc ccgcgaccag cgtgggagcg tttcgctgct gagttgagct
     1681 atcgccgagc tgcccaaggc cattgtcgcc ccagcagtag gctgcgtttg ccgtcgtcac
     1741 tccgcacgtc atgccgtgcc ccgcgctgag ctgttgaaac acaatgtcac ggggtgacgt
     1801 ccacccacgc cgagttcctc gcactcgatt cgaccgccgc atggatgaac ttgacgccgt
     1861 ccgcgccatc ttccaccgtc ggcacgagca acgccgcccg atccggcttt cgcatcgcgc
     1921 gacgcgcgcg gatctgctcc gccagatcgc gatacagatt ggcgaacgcc tcgagatagc
     1981 cctccggatg acccgacggg atgcgggtcg cgagggccgc ggcggagccg agattcggac
     2041 caccacgcga taacacgcgc ggcgcttggc cgaccggcgc gaagcgcagt tggttggggt
     2101 gctcctgctg ccattccagg cccgcctggt cgccgtacac gcgcacgcgc aacccgtttt
     2161 cattccccgg cgcgacctgg ctgctccata acatgccacg cgcgccgttc tcgaacctga
     2221 gcaggatgtg gacgttgtcg tcgagcttgc ggcccggcac gaaccggctc aggtccgccg
     2281 caagctcgcg cgcggccagg ccggtgacga agcccgcgag attgtacgcg tgcgtgccga
     2341 tgtcgccgag gcagccgccg gcgccggcgc gcgcgggatc gacccgccac gcggcctgct
     2401 tctgccccgt gtcttcgagc ctggtcgtga gccaatcctg cacatactcg acctgcacga
     2461 cgcggatcgt ccccagctcg ccggccgcga tcatctcgcg cgcctggcgc accatcgggt
     2521 atccggtgta gttgtgcgtg acgccgaaga tgagaccggt gcgccgcacc gcctgcacca
     2581 agtcgagtgc atcctcgacg gtggtggtca tcggcttgtc gcagatcacg tcgataccgc
     2641 gatcgaggaa ggcgcgggcg atcgcgtggt ggctgtcgtt cggcgtgacg atgctcacga
     2701 cttcgatccc gtcgggccgc tgcgcttcgg ccgccgccat ctcgacaaaa ctcgtgtaga
     2761 ctcgggtcgg cgcgagctgg agatcggcgc ccgattccct ggcgcgcact ggatcggacg
     2821 agagggcgcc cgcgaccagc tcgtactgcc cgtccatgcg ggcgcacagc cgatgcacgc
     2881 cgccgatgaa cgcgccgcgt ccaccgccca ccatgccgag ccgcagcggg cgagcaggtc
     2941 cggactccac ggtcgcctcc accatgtcag gcgacgccga gcatgcggcg gttggcggcc
     3001 tcgtcggtgt gggcgccgct gaaatcgtcg aacgcctttt ccgtgacacg gatgatgtgg
     3061 tcggcgataa gcttcgcgcc ttcgcgggcg ccgtcctccg ggtgcttgag gcagcactcc
     3121 cactcgagca ccgcccaccc ggcatagtcg tactgtgcca gcttcgagaa gatggccttg
     3181 aagttcactt gcccgtcgcc cggggaacgg aaccggccgg cgcgattgat ccacggctgg
     3241 tacccgccat agacgccctg gcggccggtc ggattgaact cggcgtcctt gacgtgaaac
     3301 atgcggatcc ggtcgtgata cagatcgatg tactcgaggt agtcgagctg ctgcagcacg
     3361 aagtgactgg gatcgtacag caggttgcag cgcttgtgcc cgtccaggcg ctccagaaac
     3421 atctcgaacg tcacgccgtc gtgcagatct tcgccgggat ggatttcgaa gcaggcgttg
     3481 acgccgtttt cgtcgaagac atcgagaatc ggtcgccagc gccgcgccaa ctcgtcgaac
     3541 gccgtctcga tgaggccggc cggccgctgg ggccagggat agatgtaggg ccacgccagg
     3601 gcaccggaaa acgtgacatg ctcggtcacc ccgagccggc gggacgcacg cgccgcgagc
     3661 ttcatctgct cgaccgccca cgcggtccgc gccgcgggct tgccgcgcac ttcgggcgcc
     3721 gcgaacccgt cgaatgcggt gtcgaacgct ggatgcacgg ccaccagctg gccctgcaga
     3781 tgcgtcgcca gctcggtgac ctggaggccg cgctcggcga ggatgccctt cacctcgtca
     3841 cagtaggtct ggctctcggc tgctttggcg aggtcgaaca ggcgggcgtc ccagctgggg
     3901 atctggactc ccttgaagcc caaccccgcg gcccaaccgg cgatggcttt cagggtgttg
     3961 aacggcggcg catcgcccgc gaattgcgcc aggaagatgg ccggcccttt gatcgttctc
     4021 atcgcgcgtc ctctcgttga gcagatgacc tcatgcgacg agaggaaata gatgctgaaa
     4081 gtagatcctt cgctacgctc ggggtcggcg gggcgcggag cgacctgctt ctatgggcgc
     4141 gctccaccgg aaggcgcccc actccatcac gagccgtcca gccggctcga tccgaatcgt
     4201 gaaccgctcg acgttccctg gcagggaatc caccgtcatg ggccggcgcg cgacgtcgcg
     4261 gtcggcgtgg tactccgtgc cccactggcc ggactggccg ttgatgatga gcgagacacc
     4321 ccccccggac cgggtcggga gcgtccacag cgtgtacgtc ccaggatgca gtggtacgcc
     4381 ggcgagttcg atcggcgccg aggtggtcag ctgcgtcgcg gcgtcggcgc cggtgcgcca
     4441 gacccggtcg taggggatca agtcgcctac cagcgttctc ccgcgcctcg ctgggcggct
     4501 atagtcgacg gtcacacggg cggcccccac cgtggcgcgg acggtgtcgc gcggctgagg
     4561 gacgttgccg ggcccgtacg tttctcatcg gccgcgaagc gcgccgcgat cgcgtcgata
     4621 tccggaacgt cggtgatgcg ccggacttct tgcttgtacg tcgttccctg ccccgagtag
     4681 gcggccagtc ggccgtgcac atccaggcgc gcggtgcccg tgcccgcaag cccggtgctc
     4741 ctgatcgcgt aggagccgtc ggggttgcgc tcgatgtccg cgtaacccac gtggccctcg
     4801 tcccagcctt cgaaaaaata ctggccgatg cgggcgcgcg tgctatccgg ctggcggcgc
     4861 gcggcatcga gcagcagctc ccaggtcgcg tacacgaacg cgttccacgg gacggtctcg
     4921 gcgtagcgct tctggtacgc gaacgaccgc atgcccgccg acgtgccgcg ggtgaacgcg
     4981 gtgaccttgt cggtgaacgt ggcggtatgg tggaggtgcc gcccggcggg tggcccgttc
     5041 gggatgtcgg tgtccatcga ccagtgcgcg atgacaccgt tcggacccag ctcggcttcc
     5101 cagtgccggc gggtcaccgt cggggagcgg ccgattgcgt cgccgatgat gcgatggccg
     5161 gtccgggtga cgcgctccac cgacgtcgta tcgttgccaa ggacggccac gaatccgtag
     5221 gtctcagggc ccgtgcagcc cccgagcacc agcagcaggg ccagccgtgc ctgaacagag
     5281 ggtgtggaca cctcaagaag gtgtggtcga attgcccggg agagaagtgg tgcgggtcct
     5341 cgcctcgcct caggatgacg aggcatgtgc ccccttccag atcacagccg cgtcatagcc
     5401 atggatcgtc cggacgacgt cggcgttcca gccctcgagg ccacgcattg ccagtggccg
     5461 cagggcgcga aggtcaatca cttcatgacc tgtggcattg gcgttcggaa gcacgcgcgt
     5521 gaggcgttgg tcgccgcttc ggaattcgtc aaactcatcg ctcgagacgg agacgaagct
     5581 ccgccctggc ccggggaccg cctgacgaga acctggacca gggaggacga ggatatggaa
     5641 agcgccaccc ccgctcatgg ccgccacttc atccgtcatt gcgccgacat cgaaaatgtt
     5701 gacataattg gcgccgcgga tcatgtggtt gtagccgaat ttcagcatga ccttaggcgc
     5761 cgatccgcgc ccccgctctt cccgtagccg cgcggccagg ttactccgtg tccattgcgc
     5821 gcggcgctgc gacgaagccc acgggctgcc ggtccgtgcc acagcgttga tggcaagtga
     5881 ttcctcgagc gtgcgcagga ttgtgtcact ctcacgatcc gggttgggcc aggcggcccg
     5941 gacggccgac accacggccg gatcctgcga aaagagaaag aggttgtccg ggctcggatt
     6001 ctgctggtgc cgcgcccagg cattcgttga cgcctccttc agacgcgcaa aagattcccg
     6061 tgcgggccgc ggcaccctgg cttcgagctt ggagattagg tagcgatcgc tgaaaatctc
     6121 acggtccagc ccccagagaa ctcgctcact cctgggcgcg gcgttgatga ccgccgcgag
     6181 gaattgagct tcctcacgca gatacatggg gctccaagtg tcgggggcag caaagaaatc
     6241 cacaatccct tggagaccat tgcgcctcgc cgccgcctca atgtcctgtg caatgatcgg
     6301 tgaaagctca atggccattc ggctgtagcc agatccacga agcgcattga agagggctgc
     6361 agcgagctgg gcggtttccg cgactccgtg ttcttcaccg atcagtgtga aacaggcatc
     6421 ccgcgcttcc tggacgagcc aatcccagcc gcgtcccgcg ggaccgtcgc tcattgtaag
     6481 gggaagccta tttccttgaa gcgcttggag caggcgcgct ggtgaactac cgggggtctg
     6541 gggctgcact cgaggaggcg cggcgccgtg cctggaaccg cgcgtacccc caagaaaaga
     6601 cgacccgcct gcgaagagta gatcgcgcct actgatcgtc atacccattg ccctccggtt
     6661 gagcattcct ccgaactcgg atagcgcagg cgggcaaaag gttgggggcg ggtacgaccg
     6721 gaatct
//
LOCUS       DBNGZW010000428         3278 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_244575, whole genome shotgun sequence.
ACCESSION   DBNGZW010000428 DBNGZW010000000
VERSION     DBNGZW010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3278)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3278)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3278
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_244575"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1144)
                     /locus_tag="AC16JC_10085"
     CDS             complement(<1..1144)
                     /locus_tag="AC16JC_10085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165083.1"
                     /translation="MRGRQLRRSAVVLTVGALAALACFENPTSPARCPDFCPAGGLAT
                     VESLLSTAIERDSSYAGYVQAYQAPVLVASSVPGVQSRPIWVSLPIVTRFRIDSGTDT
                     TTGPIVVDSMKLTVTVLRADTAAVHNLRLAFYQLPVGIDTTTTFADLAAAFSGTPLRV
                     INVDSIAALPSRRDTITGDTVLAIDSARNRVALRIKFTGSQTGFSAADSGKLGLGVIA
                     TGDNPPSIALGSVESGLGATAWWFYRVDSAGKLIRPDSLPLKAPDSLAAPPSFDSFVF
                     DAPPVNLDANLTVGGVHSIRSLLRFTLPRGLRDSTQIIRATLQLVPAGAAVTTGDSVF
                     LRVKRLIADLGAKSPVVVDTLVASSPPFVGVPSDTVRIEMTTMFRLW"
     gene            complement(1141..1527)
                     /locus_tag="AC16JC_10090"
     CDS             complement(1141..1527)
                     /locus_tag="AC16JC_10090"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011821998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR5165084.1"
                     /translation="MSDELVRRARAVMENAYAPYSNFHVGAAIEADDGTIHVGCNVEN
                     ASYGLTICAERMAVGAAVAAGKWRLVRIAVASGVEPPATPCGACRQLLAEFGLDLEII
                     ASGPTSRRRWRLRELLPEAFTREALA"
     gene            complement(1524..2687)
                     /locus_tag="AC16JC_10095"
     CDS             complement(1524..2687)
                     /locus_tag="AC16JC_10095"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011344855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - metabolic compound salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HIR5165085.1"
                     /translation="MKRAAIIVLDGLGIGATPDQDAYGDAGSDTLGNVAQAVGGLRLP
                     NLERLGLGLCRPLRGMARPTAPGAAHGVALPRSHGKDSTTGHWEICGVLLERAFRTYP
                     EGFPVELLEEFSRRTGRGWLGNKAASGTAIIDELGAEHQRSGKWIVYTSADSVFQVAA
                     HETTVPLAELYRACATAREMLIGEHAVSRVIARPFEGTPGDYRRTARRRDFSIEPVGT
                     TLLDRLAQAGVARIGIGKVDDLFAGRNIVSEHTPTNGDAYRLIEGALDTLETGFIFVN
                     VIEFDQTWGHRNDVPGFHQGLRELDAWIPRLEARMREDDLIIMTADHGNDPTTPSTDH
                     SREAVPVLALGPRVRPVSLGERATFADLGATVGEYFGVAPLAAGRSFLPEVRA"
     gene            complement(2684..>3278)
                     /locus_tag="AC16JC_10100"
     CDS             complement(2684..>3278)
                     /locus_tag="AC16JC_10100"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013038.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006522 - obsolete alanine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR5165086.1"
                     /translation="ECADGSVQRQLERFHAALAGLSRPPLLRPLANSAAAMTTPAAAL
                     DLVRPGVFLYGGSPGGGIAPGRPVVSIRARVVSVRRLRAGETVGYNARWTASRDTTIA
                     TLGMGYADGVPPALGSGGRGSVLLGGTRRPIVGTVSMDVVMVDAGDLAVRVGEVATLL
                     GEAGGQSLTLAEVSEASGVQPRGLLTALGRRLPRLYE"
BASE COUNT          510 a         1240 c         1072 g          456 t
ORIGIN      
        1 gccacagacg aaacatggtg gtcatctcga tgcgcaccgt gtcggagggg acaccgacga
       61 acggcgggga gctggcgacg agggtatcga ccaccaccgg cgacttcgct ccgaggtcgg
      121 cgatcagtcg tttcacccgg aggaagaccg aatcgccggt cgtcaccgcg gcgccggccg
      181 gcacgagctg cagcgtcgcc cggatgatct gcgtcgagtc acgcagtccg cgcggcagcg
      241 tgaaccgcag cagagagcga atcgagtgca cgccgccgac ggtgaggttc gcgtccaggt
      301 tcaccggggg tgcatcgaag acgaacgaat cgaagctcgg cggggcggca agggagtccg
      361 gcgccttgag cggcagcgaa tccgggcgaa tcagtttacc ggccgaatcg acgcgataga
      421 accaccaggc ggtggctccc agcccgctct cgacggatcc cagcgcaatg ctcggagggt
      481 tgtcgccggt ggcgatgacc cccagaccga gtttcccgct gtccgcagcg ctgaagcccg
      541 tctgactgcc ggtgaacttg atgcgcagtg ccacccggtt gcgcgccgag tcgatcgcga
      601 gcacggtgtc tcccgtgatg gtgtcacggc gactcggaag cgcggcgatg ctgtccacgt
      661 tgatcacccg cagcggcgtc ccggagaacg cggccgccag atccgcgaag gtggtcgtgg
      721 tgtcgatgcc gacgggcagc tggtagaacg cgagccggag gttgtggacc gcggccgtgt
      781 cggcccgcaa caccgtcacg gtgagcttca tcgagtccac cacgatcggg ccggttgtcg
      841 tgtcggtccc gctgtcgatg cggaatcgcg tgacgatggg aagcgagacc cagatcggcc
      901 gagactggac acccgggacg ctcgacgcga ccagcaccgg cgcctgatac gcctgcacgt
      961 accctgcgta cgaggagtcc cgctcgatcg ccgtcgaaag cagtgactcg accgtcgcga
     1021 gcccgccggc cggacaaaag tcggggcacc gcgccggcga ggtcgggttc tcgaagcagg
     1081 cgagggccgc cagcgccccg accgtcaaca ccaccgcact gcgccgcagc tggcgccccc
     1141 tcatgcgagt gcctcgcgcg tgaacgcctc gggcaacagc tcgcgcagcc gccagcgccg
     1201 ccgactcgtt ggacccgacg cgatgatctc cagatcgagc ccgaactcgg ccagcagctg
     1261 gcggcaggct ccgcacggcg tggccggcgg ctcgaccccg ctggccaccg cgattcgcac
     1321 caggcgccac ttccccgccg ccaccgcggc gcccacggcc atccgctcgg cgcagatggt
     1381 gagcccgtac gacgcattct cgacgttgca gcccacgtgg atcgtcccgt cgtcggcctc
     1441 gatcgccgca ccgacatgga agttgctgta cggtgcgtag gcgttctcca tcaccgcccg
     1501 cgcccgccgc accagctcgt cgctcatgcc cggacctccg gcaggaacga ccgtcccgca
     1561 gcgagcggcg caacgccgaa atactcgccc accgtggccc ccaggtcggc gaaggtcgcg
     1621 cgctccccca ggctcaccgg acggacccgc ggccccagcg cgagcaccgg cacggcttcg
     1681 cgcgaatgat cggtggacgg cgtggtcgga tcgttgccgt ggtccgcggt catgatgatc
     1741 aggtcgtcct cccgcatccg cgcctcgaga cgtggtatcc aggcatccag ctcgcgcagt
     1801 ccctgatgga accccgggac gtcgttgcgg tgcccccacg tctggtcgaa ctcgatgacg
     1861 ttcacgaaga tgaatcccgt ctccagcgtg tcgagcgccc cttcgatcag ccggtacgcg
     1921 tcgccgttgg tgggcgtatg ctcgctcacg atgttccgtc ccgcgaacag gtcgtccacc
     1981 ttgccgatcc cgatgcgtgc cactcccgcc tgcgcgagcc ggtcgagcag cgtcgtgccg
     2041 accggctcga tcgagaaatc cctgcgccgc gccgtccggc gatagtcgcc gggcgtcccc
     2101 tcgaacggcc gcgcgatcac ccggctcacg gcgtgctccc cgatcagcat ctcgcgcgcg
     2161 gtggcgcacg cccggtacaa ctccgccagc ggcaccgtgg tctcgtgcgc cgccacctga
     2221 aagaccgagt ccgccgaggt gtaaacgatc cacttcccgc tgcgctggtg ctcggcaccc
     2281 agctcgtcga tgatcgccgt gcccgacgcc gccttgttcc ccagccatcc ccggccggtg
     2341 cggcgcgaga actcctccag cagctccacc ggaaacccct ccggataggt gcggaaggcc
     2401 cgctccagca gcacgccgca gatctcccag tggcccgtcg tgctgtcctt gccgtgactc
     2461 cgtggcagcg ccaccccgtg ggcggcgccc ggcgcggtcg gccgagccat gccgcgcagc
     2521 ggccggcaca aacccagccc gagccgctcc agattgggga gccgcaggcc acctaccgcc
     2581 tgcgccacgt tgccgagcgt atccgagccg gcgtcgccgt aggcatcctg gtccggagtg
     2641 gccccgatgc ccaatccatc cagcacgatg atggctgccc gcttcattcg tacagccggg
     2701 gcagccggcg ccccaacgcc gtgagcaggc cgcggggttg taccccactc gcctccgaaa
     2761 cctcggccag ggtcagcgac tgccccccgg cctcgcccag cagcgtggcg acctcgccga
     2821 cgcgcaccgc cagatccccg gcgtccacca tcaccacgtc catcgacacg gtcccgacga
     2881 tggggcggcg cgttccgccg agcagtaccg acccacgccc tccggatccg agtgccggcg
     2941 gcaccccgtc ggcgtagccc atccccaggg tcgcgatcgt cgtatcccgc gacgccgtcc
     3001 accgggcgtt gtagcctacc gtctcgccgg cgcgcaatcg ccgcacgctc acgacgcgtg
     3061 cccggatact cacgaccggc cggcccggcg cgattcctcc gcccggtgat cctccgtaca
     3121 gaaatactcc cggccgcacc aggtcgagcg ccgccgccgg cgtggtcatc gcggccgcgc
     3181 tgttggcgag ggggcgcagc agcggcggcc gcgacagccc ggccagggcg gcgtgaaacc
     3241 gttcgagctg gcgctgcacc gacccatcgg cgcactcc
//
LOCUS       DBNGZW010000429         3905 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_519115, whole genome shotgun sequence.
ACCESSION   DBNGZW010000429 DBNGZW010000000
VERSION     DBNGZW010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3905)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3905)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3905
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_519115"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..570)
                     /locus_tag="AC16JC_10105"
     CDS             complement(<1..570)
                     /locus_tag="AC16JC_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165087.1"
                     /translation="MPRRDAVYTIDAADEAAGPWPATRAWRAEAASSPGTPPFLARRV
                     WVAVLVLYVILAILEPYDLLSPISGYGLQEHKAEIGAALDTIQGGSMLRRVGVLALFL
                     YGAVAVFVSRRAHRRYHPAIAVPAVALFLLAMASPLWSEDPAITTRKVVVLFALVLTV
                     FGVARCWEFDTIIRVAFVLSGLTILLGVAT"
     gene            complement(570..1400)
                     /locus_tag="AC16JC_10110"
     CDS             complement(570..1400)
                     /locus_tag="AC16JC_10110"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019489705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008194 - UDP-glycosyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HIR5165088.1"
                     /translation="MNDSGDPVRCGSAAVIESYRLLGVRVHALSLRTLTDAVREAVRS
                     GQRRIIANHNLHSIYLQRRDAKLRAFYARAHLVHVDGMSIIFAGRLLGLPLRREHRVT
                     YVDWIDPLLATASGQGWRVMYVGANGSVGDRGAAALRRRHPGLVLETLHGFFDPAPDG
                     DENRAVVRHIQVFAPHLLMVGMGMPRQEHWILDNLERLPPCVVLTAGAAITYAAGSVP
                     TPPRWAGAYGLEWAFRLLAEPRRLWKRYLLEPWFVLALLARELVAGRARQDLDSGRWH
                     "
     gene            complement(1397..2701)
                     /locus_tag="AC16JC_10115"
     CDS             complement(1397..2701)
                     /locus_tag="AC16JC_10115"
                     /inference="COORDINATES: protein motif:HMM:NF014051.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR5165089.1"
                     /translation="MRSFFLFPERGLATGAAWMLGAQGIRVLSQVAYFVLLARRLGAE
                     GYGAFAASLGAVALAVPFAGMGAGSFLIRGVTRGLHTPDHWWGRTVATVARTGSILAL
                     GVVGLGWLVLHDRIPFALLLGVTVSDVVLLPFIEVSGQAFQALGRLDRTALVWVVWST
                     LKVAAAITLSIVPAASLPLWAVLYPLSTAGAALVAVASVTRRVGWPRHPRSFGKAEAR
                     EGLLFSLSGSARSVYDDIDKAILARLGTLGAAGIYGAAYRIIDVAFLPVRSLVFAAYP
                     RFFARGAAGVEGAAALARSLVSPAGAYSLAAGAALFLLAPLVPVVLGPSYAGAEGAIR
                     WLAILPLFRSLHYFAADALTGAGHQGWRTAAQIGVAVLNVVLNLILIPAYSWEGAAWA
                     SLASDATLALSLWLILTGLARRAADPLPAAGAVSLVAERPSP"
     gene            complement(2738..3370)
                     /locus_tag="AC16JC_10120"
     CDS             complement(2738..3370)
                     /locus_tag="AC16JC_10120"
                     /inference="COORDINATES: protein motif:HMM:NF014606.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HIR5165090.1"
                     /translation="MSRYMVVVLAALGALPAARAWSQGTAAAYPNTGSVEEPLAPGDA
                     IQLAFWREPQLGGEFPVDENGTVVLPLLGVRSVARLPATQLRRTLLEEYAQQIRNQDV
                     RITLLRRVRILGAVKNPGLYRVDPTMTVVDALALAGGATPQGKPRDVQVYRDGHQVRS
                     HLDANTQLLQQVRSGDQIVVPERSWFARNGGYVVAAGISAIGIIVARAVP"
     gene            complement(3370..>3905)
                     /locus_tag="AC16JC_10125"
     CDS             complement(3370..>3905)
                     /locus_tag="AC16JC_10125"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004713 - protein tyrosine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004715 - non-membrane spanning protein
                     tyrosine kinase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HIR5165091.1"
                     /translation="CNLALTRASHGVRTLLIDADMRTNGVARFFRSPTPNYGLSDLLA
                     GSADLSTVCTRLRVSGRDTLGVIPAGTPTPHSAELLESPSMAALLEDARREFDLVVID
                     TPPLNAITDAAPLAAMVDGVVLVVRGGMTDREALELALNRLARLNGRVLGVVFNDVRL
                     PQAYVKRSGAAFAEWRS"
BASE COUNT          688 a         1407 c         1351 g          459 t
ORIGIN      
        1 cgtcgctacc ccgagcagga tcgtcaggcc gctcagcacg aacgcgaccc ggatgatcgt
       61 gtcgaactcc cagcagcggg ccactccgaa cacggtcagg acgagcgcaa acaacaccac
      121 gaccttgcgc gtcgtgatcg cgggatcctc gctccagagc ggactcgcca tggcgagcag
      181 gaacaacgcg accgcgggaa cggcgatggc cggatggtag cggcggtggg cgcgccggga
      241 gacgaacacg gcaactgcac cgtagagaaa gagggcgagc acgcccaccc ggcgcagcat
      301 gctcccgccc tggatcgtgt cgagggcggc accgatctcc gccttgtgct cctgaagccc
      361 gtagccggaa atcggcgaca gcaggtcgta gggctccaga atcgcgagaa tgacgtagag
      421 cacgagcacc gcgacccaca ctctcctggc caggaagggg ggagtgcccg gcgaggaggc
      481 ggcctcggcg cgccacgccc gcgtcgccgg ccacggcccg gccgcctcgt ccgctgcgtc
      541 gatggtgtag accgcgtccc gacggggcat cagtgccacc gcccggaatc gagatcctgc
      601 cgcgcccggc cggccaccag ctcccgcgcg aggagcgcca ggacgaacca cggctccagc
      661 agataccgct tccagagacg ccggggctcg gcgaggagcc ggaacgccca ctccaggccg
      721 taggccccag cccagcgcgg aggtgtcggc acgctgcccg cggcgtaggt gatcgcggcg
      781 ccggcggtca gcaccacaca gggagggagc cgctcgagat tgtcgagaat ccagtgctcc
      841 tggcgcggca tccccattcc caccatcagc aggtggggcg cgaagacctg gatgtgccgc
      901 accaccgcgc ggttctcgtc cccgtccggg gccgggtcga agaagccgtg caacgtctcg
      961 agcaccagac cgggatgacg gcgacggagg gcggcggcgc cgcggtcacc cacgctcccg
     1021 ttcgccccga cgtacatcac ccgccacccc tggccggagg ccgtcgccag caacgggtcg
     1081 atccagtcga cgtaggtgac ccgatgctcg cgacgcagcg gtaatccgag aagtcgaccc
     1141 gcgaagatga tcgacatgcc atcgacgtgc acgagatgcg cacgcgcgta gaaggcgcgc
     1201 aacttcgcgt cgcggcgctg cagatagatg ctgtgcagat tgtggttcgc gatgatacgc
     1261 cgctgccccg accgcacggc ttcccgcacg gcgtcggtga gggtcctcag ggagagcgca
     1321 tgcacgcgaa cgcccagcag ccggtacgac tcgatcaccg cggcgctgcc gcagcggacg
     1381 ggatcgccgg aatcattcat ggcgatggcc gctccgccac gagggacacc gcgcccgcag
     1441 ccggcagcgg atccgcggcg cggcgcgcga gaccggtcag gatcagccag aggctcagcg
     1501 ccagcgtcgc gtcggacgcg aggctcgccc acgccgctcc ctcccaggag tacgccggga
     1561 tcaggatcag gttgaggacg acgttcagca ccgcgacgcc gatctgggcc gcggtgcgcc
     1621 atccctgatg gccggcgccc gtcagggcat ccgcggcgaa gtagtgcaga gagcggaaca
     1681 gcggcaggat cgcaagccag cggatcgccc cctcggcgcc cgcatacgat ggacccagca
     1741 cgacggggac gaggggggcg agcaggaaga gcgcggctcc ggccgccagg ctgtaggcgc
     1801 cggccggcga taccaggctc cgcgccagcg cagcagcccc ttccaccccg gcggcccccc
     1861 gggcgaagaa tcgcgggtag gcggcaaaga ccagcgagcg gaccggcagg aaggccacgt
     1921 cgatgattcg gtaggcggcg ccgtagatgc cggccgcgcc aagggtgccg agcctcgcca
     1981 gtatcgcctt gtcgatatcg tcgtacaccg agcgggcgct gccgctcagg gagaacagca
     2041 gtccctcgcg cgcctcggcc tttccgaaac tccgcgggtg ccgcggccat cccacccgac
     2101 gcgtcacgct cgcgaccgcg acgagtgccg cacccgccgt ggaaagcgga tagagcaccg
     2161 cccacagagg cagactcgcc gccgggacga tcgacagggt gatggcggcg gcgaccttca
     2221 aggtgctcca gaccacccac acgagcgcgg tgcggtcgag tcggcccaat gcctgaaacg
     2281 cctggccgct gacctcgatg aacgggagca gcacgacatc ggacacggtg acgcccagca
     2341 gcagcgcgaa gggaatccgg tcgtgcagta ccagccagcc gagccccacc acccccaggg
     2401 cgaggatcga tcccgtccgg gccacggtcg ccacggtccg gccccaccag tgatccgggg
     2461 tgtgcagccc ccgggtcaca ccgcggatga ggaagctgcc cgcccccatc cccgcgaacg
     2521 gcaccgccag ggcgaccgcg ccgagcgacg cggcgaacgc cccgtacccc tcggctccca
     2581 gtcggcgcgc cagcaggacg aagtacgcca cctgtgacag cacgcggatc ccctgggcgc
     2641 cgagcatcca ggcggccccg gtagcaagcc cgcgctcggg aaacaggaaa aaggatcgca
     2701 accggatttc gagacctcgc tggtgaagcc cgcgccgtca gggaacggct cgagcgacga
     2761 tgatgccgat cgccgagatg cccgccgcga cgacgtagcc cccgttgcgg gcgaaccagc
     2821 tccgctccgg gaccacaatc tgatctcccg agcgcacctg ctgcaggagc tgcgtgttcg
     2881 catccagatg cgagcgcacc tggtgcccgt cgcggtacac ctggacgtcc cgcggcttgc
     2941 cctgcggcgt cgcccccccc gccagggcca gggcgtctac gaccgtcatg gtcgggtcca
     3001 cccggtagag acccgggttc ttgacggccc ccaaaatgcg gacccggcgc agcagcgtga
     3061 tgcgcacgtc ctggttgcgg atctgctgcg cgtattcctc cagcaaggtc cgacgcagct
     3121 gcgtcgccgg cagccgggcc accgagcgca cgccgagcag cggaagtacc accgtgccgt
     3181 tctcatctac cgggaactcg ccgcccagct gcggttcgcg ccagaacgcc agctgaatgg
     3241 cgtcgcccgg agcgaggggc tcctcgacgg aaccggtgtt cggatacgcc gccgcggtgc
     3301 cctggctcca tgccctggcc gccggcaggg cccccaaggc ggcgaggacg acgaccatgt
     3361 atctgctcat caggacctcc actcggcgaa cgcggcgccg gaccgcttga cgtaggcctg
     3421 cggcagccgc acgtcgttga agaccacgcc aagcacgcgg ccgttcaggc gagcgagacg
     3481 gttcagggcc aattcgaggg cctcgcgatc cgtcatcccg ccccgcacca ccagcacgac
     3541 gccgtcgacc atggcggcga gcggcgcggc gtcggtgatg gcgttgaggg gcggcgtgtc
     3601 gatcacgacc agatcgaact cccgcctggc gtcctcgagc agcgccgcca tgctcgggga
     3661 ttcgagcagc tcggcggagt gcggggtggg agtgccggcg ggaatgactc ccagcgtgtc
     3721 ccggccactc acccggagcc tcgtgcacac ggtcgagagg tccgcggaac cggcgagcag
     3781 atcgctcagc ccgtaattgg gcgtggggga ccggaagaag cgcgcgacgc cgttcgtccg
     3841 catgtcggca tcgatgagga gcgtgcggac gccgtgactg gcccgcgtca gcgcgaggtt
     3901 gcagg
//
LOCUS       DBNGZW010000430        13057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_98169, whole genome shotgun sequence.
ACCESSION   DBNGZW010000430 DBNGZW010000000
VERSION     DBNGZW010000430.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 13057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13057
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_98169"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1630
                     /locus_tag="AC16JC_10130"
     CDS             <1..1630
                     /locus_tag="AC16JC_10130"
                     /inference="COORDINATES: protein motif:HMM:NF019556.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HIR5165092.1"
                     /translation="WEAPDNELRGHYTGHYLSALALMSASQGDGELKARGDRMVAELA
                     QCQNAHGYGYLSAFPVEFFDRLRAGKPVWAPFYTVHKIMAGLLDMHTLAGNAQALDVL
                     RGMASWVASWTQPLGDYEMARVLEREYGGMNEVLYNLSEVTGQDRWRDVAHRFDHERF
                     FAPLALGRDELKGLHANTNIPKVIGAARRYELTGELRYRRIAEYFWREVTTQRAYCTG
                     GTSNGEGWETEPGVLSTQLSGYTQEDCTTYNMLKLTRHVFGWTADPAAADYYERALFN
                     GILGSQHPRDGEKLYYVSLASGLWKLFGTPTQDYWCCTGTMSEAFAKLGDSIYFQDDA
                     GVFVNLFIASELEWGVRGVRLEQRTRFPEEEGTTLVVHARRPVRFALRIRVPSWMAAD
                     GTARLNGRALEGFAAPGGYYVLDRTWKDGDTVAVTLPMRLHLQAMPDDPSLQAVMYGP
                     LVLVGRLGTAGLTPDVLRAEPTRPRTVPEYKSEPFPAPAFTANGSGPADWITRTTARS
                     LEFRTTGQHQDVTLVPFHTLFDERYAVYWKITAA"
     gene            1627..2919
                     /locus_tag="AC16JC_10135"
     CDS             1627..2919
                     /locus_tag="AC16JC_10135"
                     /inference="COORDINATES: protein motif:HMM:NF016763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HIR5165093.1"
                     /translation="MNTAGARLGVLAARLAYVGVLAVATLAPFHFGVPPDGIGPGLRN
                     AFTFRYSLATAVDAVQNVLLFAGWGALWVTTSRPGALARGIRVSALTGAAMSMLAEAL
                     QLFLPDRRASLLDVMTNTAGAALGALAIALAVQIADAMRRQKSYIGLPAASFAIAYLS
                     AAVTEAVLPLQETAVIATRAGTQLRRMRTVSPRLDWSTLWHVPPVDILLFVPLGVFGV
                     MALVESGMERWAAVRQVSAWGALGAVLLELTHGPLGQPIELGTALAHLVGVAIGAVAC
                     SLWLPGFSRLVRGRGRPAVVLVAYALLIAFWAWRPFAIETDPAVIHQQFALERLMPLY
                     GLARRSDLASVAEIVKPFFLFFPLGALLAVWPLRRSGILGFCLPALYLAAVTETLQGF
                     ISGRFFDGTDLVIQCAAAAIGWAVVRQAGYEPHGTLLG"
     gene            2950..4671
                     /locus_tag="AC16JC_10140"
     CDS             2950..4671
                     /locus_tag="AC16JC_10140"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013061391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR5165094.1"
                     /translation="MLGIRMRLLPAALLVWCPAAAAQGRSADLVLLHGTIVTVDATHP
                     SATALAVSGDTVLAVGTDAEIQRYVGRATLVLDLHGAVVMPGFIESHGHFLGLGESKM
                     QLDLTKAASWDEIVRMVAAAVQTVKPGAWILGRGWHQEKWNTPPQPNVAGLPVGDDLA
                     AVSPDNPVLLTHASGHAVFVNRKALELAGIGADTKDPPGGEIVRDSTGRPIGMLRDRA
                     AGPVRQAYAASQAGRTPAEVRAHLERAVELAAQDALAKGITAFQDQGESFATIDVLKD
                     LAARGKLPIRLYAMVGGAPLDTLAAQLAAHRIVGFAHDHFTVRAVGEITMDGALGTHS
                     AWFLEPYDDLPSSSGFPVTTPDQLRRIAELALHNGYQVAPHAIGDRANHETLDVFAAL
                     EAEHPEIKTARWRIEHAQHLAPADIPRFAALGVIASMQGIHACSDGPYVVKRLGERRA
                     IDGAYVWRKLLATGVVIANGTDVPVEDEDPIANVHCSVTRRMKDGALFYPDQKMTRAE
                     AIASYTRDAAYAMFQEDRLGSLAPGKLADITVLDRDLMTVAEGRIPGTRVLYTIVGGK
                     IVYQAQR"
     gene            complement(4708..4789)
                     /locus_tag="AC16JC_10145"
     tRNA            complement(4708..4789)
                     /locus_tag="AC16JC_10145"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4753..4755),aa:Leu,seq:gag)
     gene            complement(4834..5382)
                     /locus_tag="AC16JC_10150"
     CDS             complement(4834..5382)
                     /locus_tag="AC16JC_10150"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR5165095.1"
                     /translation="MEQRIGFGLRLGALLLDCILVFVLSWLGAGTIGGLLGGTAASMA
                     ADAMARADTTQVNMAVVGGILGAVIGMVIAFAVIGTLYFLVEGFTGFTLGKLILGIRI
                     ANADGTAASVPTLLGRYLIKNCYYVLSLLGIVTGVSALGTLGTVGGLIIIVGCFFTLG
                     TARQAFHDMIMRTAVYPKAAIK"
     gene            complement(5449..6312)
                     /locus_tag="AC16JC_10155"
     CDS             complement(5449..6312)
                     /locus_tag="AC16JC_10155"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5165096.1"
                     /translation="MDIALVTYRQLPDLHPDDHPLRHALQSLGAAVHAVCWDEAALDW
                     AAFDAVVLRSCWDYYLRFGEFRRWLDGLERAGTRLWNPAGVVRWNSDKIYLADLAGDG
                     VLLPGTEWLAPGERRDLGAVLEARGWDQAVVKPRVSASGHETWLTDRRRAAGDQPRVA
                     RLVAGEGALVQEFVPEVMTAGELSLVYLAGEFSHAVRKRAAPGEFRVQERFGGWTEPA
                     TVRAAALEAGKRALARVPGPWLYARVDGVERGASRQFLVMELEVFEPTLFLRGAPEAA
                     RRLARAIVSIP"
     gene            6425..6685
                     /locus_tag="AC16JC_10160"
     CDS             6425..6685
                     /locus_tag="AC16JC_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165097.1"
                     /translation="MRRSGRTAQQRAAEALSAKEAARLQSAAEQLARAVEEFLRRRVP
                     LHSAPECREGQVELLDWSAAGCESLCPLCLLNRALAIYRMRA"
     gene            6695..7429
                     /locus_tag="AC16JC_10165"
     CDS             6695..7429
                     /locus_tag="AC16JC_10165"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007867777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HIR5165098.1"
                     /translation="MTPDPHSPTRHPVVPRAQWLAARRALLREEKEFTRRRDALSRRR
                     RDLPWEAVEKSYVFDGPEGPASLTDLFAGKSQLVVYHFMFNPSDDAGCPHCSFWADHY
                     DGMLPHLRQRDVSFVVISRAPLEKLAAFQRRMGWKFKWLSSGRNEFNYDFGASFTPDE
                     VKSGKPFYNVGTLPPGVEDREGLSVFFKDADGRIFRTYSCYARGIDMFNGTYQILDLV
                     PKGRDEDPEATQSWVRHHDRYGEQRS"
     gene            7510..7845
                     /locus_tag="AC16JC_10170"
     CDS             7510..7845
                     /locus_tag="AC16JC_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR5165099.1"
                     /translation="MDQTNTDVIQGTLDLLILKTLSLEPMHGFGIGRRVEQISRGVFK
                     VNPGSLLTALQRLERAGWLDAQWRQTENARRAKFYSLTRAGRKQLDVETADWARRAGA
                     IARLLNAEG"
     gene            7850..10489
                     /locus_tag="AC16JC_10175"
     CDS             7850..10489
                     /locus_tag="AC16JC_10175"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR5165100.1"
                     /translation="MSLWRQVIRGLRALTNRAATDQDVTDEVSDYLAHATAAHLARGL
                     PRDAAVREARLELGNVTAAREQVREYGWENLIESALTDLRYGVRRLRQAPGFAEITVL
                     TLALGLGATTAIFSAVDPILFASLPYPHAGRIAAILEVTNSGGRTGGTFGLYRAFVER
                     SRSFAAIAVFKPWQPTMTGADQPERWAGQRVSASYFQVLGVAPAVGRGFDPSDDRRNG
                     PNVVVLSDVLWRRRFAGDRAIVGRQIRLDDNLYTVIGVMPRGFENVLAPATDVWALLQ
                     YDASLPAHGREWGHHLRTVGRLQPGVSVDQATTEINALGHSVVETLHPETYDPDARFA
                     LASLRAELTRGVKPALLAMLGAVLLVLVIACVNVTNLLLARGVLRRPEFALRAALGAR
                     HGRLIRQLLTESLVLAAVGGAIGMAAALLGVRALQALAPPDLPRLGAIGVNGAVFAFG
                     LGITTLIGLAFGVFPALQAARQDPHGDLQHGTWRTAGPHRRTPNALVIAEVALALVLL
                     VGSGLLLRSLQHLFAVPVGYDSPGLLTMQVQTVAHRFDQDSAAVRFFERTLEAVRSVP
                     GVTAAALTSQLPLSGDRDEYGAAFEVSPTQPAERYGVFRYAVSPGYIEAMHIPLRSDR
                     PLQEGDRPDAPLVALISESLAHSRFQGTDPIGKRLHIGPAGPYTIVGVVGDVKQMSLA
                     LNESDAVYIPASQWMFADRVLSIVVRTRGDAAALAPAVRDAVWSVDKDQPVVRVATMD
                     HLLAASAAERHFALVLFEAFALAALVLAAAGIYGVLSGSVAERTREIGVRAALGASQT
                     EILGLVVRRGMALTGLGVALGLVGAVIASRALITLLFGVSRLDPVTYAGVVLVLVFVA
                     ALASGIPAWRAVRIDPAIALRSE"
     gene            complement(10500..11996)
                     /gene="pdxR"
                     /locus_tag="AC16JC_10180"
     CDS             complement(10500..11996)
                     /gene="pdxR"
                     /locus_tag="AC16JC_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070279 - vitamin B6 binding [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:2000142 - regulation of DNA-templated
                     transcription initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MocR-like pyridoxine biosynthesis transcription
                     factor PdxR"
                     /protein_id="HIR5165101.1"
                     /translation="MEHDIRPIAERGRRTPVWFALFQSHPGPARSLRDRICAAVRDAV
                     QSGRVGHGERLPASRIMARDLTVSRITVEAAYAQLEAEGYLRRRVGDGTFVSVDVGPH
                     PPAVKPAVRRRLETASWSARGGRIVRGGGCVDPTSAMAFSAGFPDLTEFPLDPWRRLT
                     ARRLRGARAALGYGDPAGESALRAAIARYLAQSRGVRCSPEQVLVLTSSQQALNLVAT
                     LLTDPGDVVWLEEPGYRGAQTALAAMGTRLTPLPVDQEGMILRAPRDGAAPRLIYVTP
                     SHQYPTGVTLSLPRRLALIEYAHRHGAWIVEDDYDSEFQYDARPLPAMQGLDARDRVI
                     YLGTFSKVLFPGLRLAYLVLPPALVSGFVTARSAHDGHSARLAQLVTADFMEEGYFAA
                     HVRRMRRLYQRRRDLLLDEIGRHIGDWCRPSGTGAGLQLTVTLPRGREAALTRAAAQV
                     GVETPGLSGRFLSRPRLDGWMLGFAALPEAAIVEGVHRLAKLRVAHRP"
     gene            12076..12909
                     /locus_tag="AC16JC_10185"
     CDS             12076..12909
                     /locus_tag="AC16JC_10185"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5165102.1"
                     /translation="MSVKRLLILLLTGSRTLTGQTPQLVDIGPLRLDVVRAGAGAPAV
                     VFEAGLGDPLNDWDRVWPAVAEFTTVVTYSRAGIGRSDPGQEEHSARRAAAELHALLA
                     RLRIPPPYVLVGRSYGGILVRLYTSLYPADVAGLVLVDGSHEQQVKRWGMLDSTYPGA
                     FRAFFDSLLGTLQAGAEAAEIQESMRIQAAAGIEGLRPLPDIPLAVLTSMQVPDSPST
                     VNQTARGHEAWRAMHDEWFRRSRNAIHIVTWRSGHAIQDAEPQLVVDAIRFVLDRVRS
                     Q"
BASE COUNT         1982 a         4656 c         4477 g         1942 t
ORIGIN      
        1 atgggaagcg cccgacaacg agctgcgcgg ccactacacc ggccactacc tgtcggccct
       61 ggcgctgatg tcggcgagcc agggagacgg cgagctcaag gcccggggcg accgcatggt
      121 cgccgagctg gcgcagtgcc agaacgcaca cggctacggc tatctgagcg cctttccggt
      181 ggaattcttc gaccggctgc gtgcgggcaa gccggtgtgg gcgccgttct acacggtgca
      241 caagatcatg gccgggctgc tcgacatgca cacgctggcg ggcaacgcac aggcgctcga
      301 cgtcctccgg gggatggcga gctgggtggc ctcctggacc cagcccctgg gcgactacga
      361 aatggcacgg gtgctggagc gcgaatacgg cggcatgaac gaagtgctgt acaacctctc
      421 cgaggtgaca gggcaagacc ggtggcgcga cgtcgcgcat cgtttcgacc acgagcgatt
      481 cttcgcaccc ctcgcccttg gccgggacga gctcaaaggc ctgcacgcca acaccaacat
      541 ccccaaggtg attggtgccg cgcggcggta cgagctgacc ggcgagctcc gctaccgccg
      601 catcgccgag tacttctggc gcgaggtcac gacccagcgc gcctactgca ccggcggcac
      661 ctcgaacggc gaagggtggg agacggagcc cggtgtgctc tcgacccagc tctcgggata
      721 cacccaggaa gactgcacca cctacaacat gctcaagctc acgcgccatg tgttcggctg
      781 gacggccgac cccgccgccg ccgactacta cgagcgcgcc ctgttcaacg gcatcctggg
      841 cagccagcat ccgcgcgacg gcgagaagct ctactacgtg agcctcgcct ccggactgtg
      901 gaagctcttc ggcaccccga cgcaggacta ctggtgctgc acgggcacca tgagcgaggc
      961 gttcgcgaag ctgggcgaca gcatctactt ccaggacgac gccggggtgt tcgtgaacct
     1021 gttcatcgcc tcggagctgg agtggggcgt acgcggggtg cgcctggagc agcgcacgcg
     1081 cttcccggag gaggaaggaa cgacgctcgt ggtgcacgcg cggcggcccg tgcggttcgc
     1141 cctccggatt cgcgtgccgt cctggatggc ggcggacgga accgcgcggc tcaacggacg
     1201 tgcgctcgag ggcttcgcgg caccgggcgg ctactacgtg ctcgaccgca cctggaagga
     1261 cggcgacacg gtcgccgtga cgctcccgat gcgactccac ctgcaggcca tgccggatga
     1321 cccttcgctg caggccgtga tgtacggacc gctggtgctc gtcggacggc tgggcaccgc
     1381 gggactgacc cccgacgtgc tgcgcgccga gccgaccagg ccgcggacgg ttcccgagta
     1441 caagagcgag cccttccctg ccccggcctt cacggcgaat ggaagcgggc cggctgactg
     1501 gatcacccgc acgaccgccc ggtcgctcga gtttcgcacg acgggccagc accaggacgt
     1561 gacgctcgtc ccgttccaca cgctgttcga cgagcgctac gccgtgtact ggaagatcac
     1621 cgccgcgtga acacggccgg cgcgcgattg ggcgtcctcg cggcgcggct ggcgtacgtg
     1681 ggggtgctgg ccgtcgccac cctcgcccca tttcatttcg gcgtcccgcc ggatgggatc
     1741 ggtcccggcc tccgcaacgc cttcacgttc cggtattcgc tcgcgaccgc ggtggatgcc
     1801 gtacagaacg tgctgctgtt cgccggttgg ggtgcgctct gggtgacgac gagccggccg
     1861 ggagcgctcg cgcgggggat ccgcgtgtcc gcgctcacgg gcgccgccat gagcatgttg
     1921 gccgaggcgc tccagctctt cctgccggac cggcgtgcca gcctgctgga cgtgatgacc
     1981 aatacggcgg gcgcggcctt gggtgccctc gcgatcgcgc tcgcggtgca gatcgccgat
     2041 gcgatgcgcc ggcagaaatc ctacatcggg ctgcccgccg cgagcttcgc catcgcgtac
     2101 ctgagcgccg ccgtgaccga ggcggtgctg ccgctccagg agaccgccgt catcgccaca
     2161 cgcgcgggca cccagctgcg ccgcatgcgc acggtctcgc cgcgactcga ttggagcacg
     2221 ctgtggcacg tgccgccggt ggatatcctg ctgttcgttc cactcggggt cttcggcgtg
     2281 atggccctgg tcgagtccgg catggaacgc tgggccgccg tccggcaggt cagcgcgtgg
     2341 ggcgccctgg gcgcggtgct cctcgagctg acccacggtc cgctcggtca gccgattgag
     2401 ctcggcacgg cgctcgccca cctcgtcggc gtcgcaatcg gtgcggtggc ctgcagtctc
     2461 tggctcccgg gcttctcgcg cctggtgcgg gggcgcggac gacccgccgt ggtgctggtc
     2521 gcctacgccc tgctcatcgc cttctgggcg tggcgtccgt tcgcgatcga gaccgatccg
     2581 gcggtcatcc atcagcagtt cgctctcgag cggctgatgc cgctctacgg cctggcccgg
     2641 cggagcgatc tcgcgagcgt ggccgagatc gtcaagccgt tcttcctgtt cttcccgctg
     2701 ggcgccctgc tcgcggtctg gccgctgcgc cggagcggga ttctgggctt ctgcctgccg
     2761 gccctctacc tcgccgccgt cacggagacc ctccaggggt tcatcagcgg gcgcttcttc
     2821 gacggcaccg atctggtgat tcagtgcgcg gccgcggcga tcggctgggc ggtcgtgcgg
     2881 caggcagggt acgaaccgca cggcaccctg ctgggctgac cgcgacccgt cgttcatttc
     2941 ggaggctgta tgcttggcat caggatgagg ctgctccccg ccgcgctgct ggtgtggtgt
     3001 cccgcggcag ccgcacaggg ccgatccgcc gacctggtgc tgttgcacgg caccatcgtc
     3061 accgtggacg cgacgcatcc cagcgcgacg gccctcgccg tctccggcga cacggtcctg
     3121 gccgtcggaa cggacgccga gatccagcgc tacgtcggcc gcgcgacgct cgtgctcgat
     3181 ctgcacggcg ccgtggtgat gccagggttc atcgaatcgc acggccattt cctgggcctg
     3241 ggcgaatcca agatgcagct cgacctgacg aaagccgcga gctgggacga gatcgtgcgc
     3301 atggtggcgg ccgccgtgca gaccgtgaag cccggcgcgt ggatcctggg acgcggctgg
     3361 caccaggaga aatggaacac gccgccgcag cccaacgtcg cagggctgcc ggtaggcgac
     3421 gacctcgccg ccgtctcgcc cgacaatccc gtgctgctga cgcacgcgag cggccacgcc
     3481 gtgttcgtga accgcaaggc gctggagctg gccgggatcg gcgccgacac gaaggatccg
     3541 ccgggcgggg agatcgtgcg tgattccacc ggccgaccca tcgggatgtt gcgcgaccgc
     3601 gccgcgggac cggtgcgcca ggcctacgcc gcgtcccagg cgggccggac accggccgag
     3661 gtccgcgccc acctcgagcg ggcggtcgag ctcgccgccc aggatgccct cgccaagggg
     3721 atcaccgcgt tccaggacca gggcgagtcc ttcgccacca tcgacgtcct gaaggatctc
     3781 gccgcccggg gcaagctgcc catccggctc tacgccatgg tgggcggggc gccgctcgac
     3841 acgctcgcag cccaattggc cgcccaccgc atcgtggggt tcgcgcacga tcacttcacc
     3901 gtccgggcag tcggcgagat cacgatggac ggcgccctgg gcacgcacag cgcctggttc
     3961 ctcgagccct acgacgatct gccgtcctcg agcggattcc ccgtcacgac ccccgaccag
     4021 ctgcggcgga tcgccgagct cgcgctccac aacggctatc aggtggcccc gcatgcgatc
     4081 ggtgaccggg cgaaccacga gacgctcgac gtgttcgccg ccctggaagc ggagcacccg
     4141 gagatcaaga cggcgcgctg gcgcatcgag cacgcccagc acctggcgcc cgccgacatc
     4201 ccgcgcttcg ccgcgctggg ggtgatcgcc tcgatgcagg gaatccacgc ctgctcggac
     4261 ggaccctacg tcgtgaagcg tctcggcgag cggcgcgcga tcgacggggc ctacgtgtgg
     4321 cgcaagctgc tcgcgaccgg cgtcgtcatc gccaacggga ccgacgtccc cgtcgaggac
     4381 gaagatccga tcgcgaatgt ccattgctcc gtcacgcgcc ggatgaagga cggcgcgctg
     4441 ttctatccgg accagaagat gacgcgcgcc gaagcgatcg cgtcctacac gcgcgacgcg
     4501 gcgtacgcga tgttccagga ggaccggctg ggctcgctcg ccccggggaa gctggccgac
     4561 atcacggtgc tcgaccgcga cctcatgacc gtggcggagg gtcgcatccc gggcacgcgg
     4621 gtgctgtata cgattgtcgg cgggaagatc gtctaccagg cgcagcggta gaaacacgaa
     4681 cggccggcgc cctcggcgcc ggccgcatgc cctggcccgg attcgaaccg ggacgccttg
     4741 cggcactgcc ccctcaagac agcgtgtcta ccagtttcac caccagggcg ggcgagagga
     4801 agctagaaac cgccgtgccg atggtcaagc caactacttg atcgccgcct tgggatagac
     4861 cgccgttctc atgatcatgt cgtggaacgc ctgcctggcg gtcccgaggg tgaaaaaaca
     4921 cccgacgatg atgatgagtc cacccacggt gcccagcgtg cccagcgcgc tcacgccggt
     4981 gacaatgccg agcaaagaca ggacgtaata gcagttcttg atcaggtagc gccccaggag
     5041 cgtcgggacg ctcgcggcgg tgccgtccgc gttggcgatg cggatgccga gaatcagctt
     5101 ccccaacgtg aatccggtga acccctccac caggaagtag agggtgccga tgacggcgaa
     5161 ggcgatcacc atcccgatga cggcgcctag gatcccgccg acgaccgcca tgttcacctg
     5221 tgtagtgtcc gcacgggcca tggcgtcggc cgccatggac gcggccgtgc caccgagcaa
     5281 ccccccgatg gtccccgcgc cgagccagga caggacgaag accagaatgc agtccagcag
     5341 gagtgccccg agccgcagcc caaagccgat ccgctgctcc atgccgccac ctccctcgca
     5401 gtgggtgagc caacgtgcgc ccccatatgc gaccctgcga ccgtcccgtc aagggatgga
     5461 gacgatcgcc cgggcgagac gccgggcagc ctccggcgcg ccccggagga acagcgtcgg
     5521 ctcgaacact tccagctcca tcacgagaaa ctgccggctc gcgccgcgct cgacgccgtc
     5581 cacgcgggca tagagccagg gaccgggcac tcgagccaac gcgcgcttcc cggcctcgag
     5641 cgccgcggcg cggaccgtgg ccggctcggt ccacccgccg aagcgctcct gcacccggaa
     5701 ttctcccggg gccgcccgct tgcgcaccgc gtgactgaac tcccccgcca ggtagaccag
     5761 cgacaactcg cccgcggtca tcacctcggg cacgaattcc tgcaccagcg ctccttcgcc
     5821 cgccacgagg cgcgccacac ggggctggtc cccggcagcc cggcggcgat cggtcagcca
     5881 ggtttcgtgg cccgaggccg agaccctggg cttcaccacc gcctggtccc agccgcgcgc
     5941 ctcgagaacg gcgccgagat cccgccgttc cccgggcgcg agccactcgg taccgggcag
     6001 caggacgccg tcacccgcga gatcggcgag atagatcttg tcggaattcc agcgaacgac
     6061 gccagcgggg ttccagaggc gcgtgccggc gcgctccagc ccgtcgagcc agcggcggaa
     6121 ttccccgaac cggaggtagt agtcccagca ggagcggagc acgaccgcat cgaacgccgc
     6181 ccagtccagc gccgcctcgt cccagcacac cgcgtgcacc gccgcgccga gcgactgcag
     6241 cgcgtggcgc agcgggtggt cgtcgggatg caggtcgggc agctggcggt aggtgacgag
     6301 ggcgatatcc acagcccaat gctaatcacc cactacgacc gatcagaagc gccggaagaa
     6361 agtcgcgcat ggtgtgccgc cccgcgcttg cgttcgggcg acggcaaacg catcctccct
     6421 gcccatgcga cgcagcggaa ggaccgccca gcagcgggcg gcggaggcgc tctccgccaa
     6481 ggaagcggcg cgactgcaaa gtgcggccga gcagctggcg cgcgcggtgg aggagttctt
     6541 gcgccgccgc gtccccctgc actcggcgcc cgaatgccgg gagggtcagg tcgagctgct
     6601 cgactggtcg gccgcgggat gcgagtcgct gtgcccgctc tgcctcctga accgggcgct
     6661 tgccatctat cggatgcggg cctgagaggt acgcatgacc ccagatcccc attcccccac
     6721 acggcatccc gttgtgccgc gtgcgcaatg gctcgccgcc cggcgggcgc tgctccggga
     6781 agagaaagaa ttcacgcggc ggcgggatgc gctgagccgc cggcggcgcg acctcccctg
     6841 ggaagccgtc gagaagtcct atgtcttcga cggaccggag ggcccggcat cgctgacgga
     6901 cctgttcgcc ggcaagagcc agctggtcgt gtaccacttc atgttcaatc cgtccgacga
     6961 cgccgggtgc ccccactgct ccttctgggc cgaccactac gacggcatgc tgccgcatct
     7021 gcgccagcgc gacgtgtcgt tcgtcgtgat ctcgcgcgca ccgctcgaga agctggcggc
     7081 gttccaacgg cggatgggct ggaagttcaa gtggctctcg tcgggccgca acgagttcaa
     7141 ctacgatttc ggcgcgtcct tcacgcccga cgaggtcaag agcgggaagc cgttctacaa
     7201 cgtcggcacc ctgcctcccg gggtagagga tcgtgagggg ttgagcgtgt tcttcaagga
     7261 tgcggacggc aggatattcc gcacctactc ctgctacgcg cgcggcatcg acatgttcaa
     7321 cggcacctat cagatcctcg atctggtgcc caagggacgc gacgaagatc cggaggccac
     7381 gcagagctgg gtgcgccacc acgaccgtta cggggagcag cgttcctgac gtcatcctga
     7441 ccgtcacatc ttgacaatca ggaggagcgg ccgccacctt tgcctcggca ctcgtactcc
     7501 ggggcatccg tggaccagac caataccgac gtcatccagg gcacgctcga cctgctcatc
     7561 ctcaagaccc tgagcctcga gccgatgcac ggcttcggca tcggccgccg ggtcgagcag
     7621 atctcccgcg gcgtgttcaa ggtcaacccg ggatctctcc tcaccgcctt gcagcgcctg
     7681 gagcgcgccg gctggctcga tgcccagtgg cgccagaccg agaacgcgcg ccgcgccaag
     7741 ttctattccc tcacgcgcgc cgggaggaaa caactcgacg tcgaaaccgc cgactgggcg
     7801 cgccgggcag gcgccatcgc gcggctcctg aacgccgagg gctaggtgca tgtcgctgtg
     7861 gcgccaggtg atccgcggcc tgcgcgcgct caccaaccgc gcggccaccg accaggacgt
     7921 gaccgacgag gtaagcgact acctcgcgca cgcgaccgcg gcccacctgg cccggggact
     7981 cccccgtgat gcggccgtac gcgaggcgcg gctggagctc ggcaacgtca cggccgcccg
     8041 ggagcaggtc cgcgagtacg gctgggagaa tctgatcgag tccgcgctga ccgacttgcg
     8101 ttatggcgtc cgccgcctac gccaggcgcc cggtttcgcg gaaatcaccg tcttgacgct
     8161 ggcgttgggg ctgggcgcca cgaccgcgat cttcagcgcg gtggacccga tcctgttcgc
     8221 gtcgctgccg tacccacacg ccggccggat cgccgctatc ctggaagtca cgaacagcgg
     8281 tggccgcacc ggcgggacgt tcggcctgta ccgcgcgttc gtcgagcgtt cgcgctcctt
     8341 cgcggcgatc gccgtgttca agccatggca gccgaccatg accggggccg accagccgga
     8401 gcgatgggcg gggcaacgcg tcagcgcgag ctacttccag gtgctgggcg ttgcgccagc
     8461 cgtgggacgg ggcttcgatc cgtccgacga tcggcggaac ggtccgaacg tcgtcgtcct
     8521 gagcgacgtc ctgtggcgcc gccgcttcgc cggggatcgc gcgatcgtcg gacgccagat
     8581 caggctcgac gacaacctgt acaccgtcat cggcgtcatg ccgcgcggct tcgagaacgt
     8641 cctggcgccg gcgaccgatg tgtgggcgct cctgcagtac gatgcgtcgc tccccgccca
     8701 cggacgggag tggggccatc acctccggac cgtagggcgg ctgcagccgg gggtcagcgt
     8761 cgatcaggcg acgacggaga tcaacgcgct cgggcactcg gtggtggaga cgctccatcc
     8821 ggagacgtat gatcccgatg cccgattcgc cttggcgtcg ctgcgcgccg agctcacccg
     8881 tggggtgaag ccggcgctcc tcgccatgct cggcgccgtg ttattggtgc tcgtgatcgc
     8941 ctgcgtgaac gtgacgaacc tgctcctcgc ccgcggcgtg ctccggcgcc ccgagttcgc
     9001 gctgcgcgcc gccctgggcg cccggcacgg ccgcctgatc cggcagctcc tgaccgagag
     9061 tctcgtcctc gccgcggtcg gcggtgcgat cggcatggcc gccgcgctgc ttggcgtgcg
     9121 cgcgctccag gcactcgctc cgccggatct gccgcgcctc ggcgcgatcg gggtcaacgg
     9181 cgccgtgttc gcgttcggac tgggcatcac cacgttgatc ggcctggcat tcggggtatt
     9241 cccggcactg caggccgcgc gccaggatcc gcacggggat ctccagcatg gcacctggag
     9301 gaccgccggc ccgcaccggc gcacccccaa cgcgctcgtc atcgcggaag tcgcactcgc
     9361 gctcgtgctg ctggtcggct cgggactgtt gctgcggagt ctccagcacc tgttcgcggt
     9421 tcccgtgggc tacgactcac ccggactgct cacgatgcag gtgcagaccg tcgcacaccg
     9481 gttcgaccag gatagcgccg cggttcgctt cttcgagcgg acgctcgagg ccgtccggag
     9541 tgtgccgggg gtcactgctg ctgcgctcac gagccagctt ccgctgagcg gagaccggga
     9601 cgagtacggc gcggcgttcg aggtgagccc gacccagccc gccgagaggt acggcgtgtt
     9661 tcggtatgcg gtgagtccag ggtacatcga ggcgatgcat atcccgctcc ggtccgaccg
     9721 cccactccag gaaggcgacc gcccggatgc tcccctcgtc gcgctgatca gcgagtcgct
     9781 ggcccacagc aggttccagg gaacagaccc cattggcaag cgcctgcaca tcggtccggc
     9841 cggaccgtac accatcgtcg gcgtggtcgg cgacgtgaaa cagatgtcgc tggccctgaa
     9901 cgagtcggac gcggtctaca tccctgcgtc ccaatggatg ttcgccgatc gggtgctgtc
     9961 gatcgtggtt cgcacgcgcg gcgacgccgc tgccctcgcg ccggcggtac gggacgccgt
    10021 gtggtcggtg gacaaagacc agccggtcgt acgcgtcgcc acgatggacc acctgctcgc
    10081 cgcttcggcg gccgagcggc acttcgcgtt ggttctgttc gaggcgttcg ctcttgcggc
    10141 gctggtactc gcggccgccg gcatctacgg cgtgctgtcg ggcagcgtcg ccgagcggac
    10201 gcgggagatc ggggtccgag cggcgctggg cgcgtcccag accgagatcc tgggcttggt
    10261 ggtgcgccgc ggcatggcgc tcacggggct cggggtcgcg ctgggcctcg tgggcgcggt
    10321 gatcgcgagc cgcgccctga tcacgctgct gttcggcgtg tcgcgcctcg acccggtcac
    10381 ctacgccggc gtcgtgctgg tgctcgtctt cgtggcggcg ctcgccagcg gcatccccgc
    10441 ctggcgggcg gtgcgaatcg atcccgcgat cgcgctccga tcggagtagc ggaccttcgt
    10501 cagggccggt gagcgactcg cagcttcgcc agccggtgca ccccctcgac gatcgcagcc
    10561 tccggtaacg ccgcgaagcc cagcatccac ccgtccaggc gtggccgcga cagaaaccgc
    10621 cccgagaggc cgggcgtctc cacgcccacc tgcgccgcgg cgcgcgtcag cgcggcctcg
    10681 cgcccgcggg gcagcgtcac cgtgagctgg agtccggccc ccgtgccgct cgggcggcac
    10741 cagtcgccga tgtggcggcc gatctcgtcg agcagcaggt cccggcgccg ctgatagagc
    10801 ctgcgcatgc gacgcacgtg ggccgcgaag tacccttcct ccatgaaatc ggccgtcacc
    10861 agctgggcga gacgcgccga atgtccgtcg tgcgcggagc gcgccgtgac gaagccgctc
    10921 acgagcgccg ggggaaggac gaggtacgcc aggcggagtc ccggaaacag gactttcgag
    10981 aaggtcccga ggtagatcac gcggtctcgc gcatccagcc cctgcatcgc gggcagcggt
    11041 ctcgcgtcgt actggaattc gctgtcgtag tcgtcttcga cgatccacgc gccgtgacgg
    11101 tgcgcgtact cgatcagcgc caggcgccgc ggcaacgaca gcgtcacccc ggtgggatac
    11161 tggtgcgacg gcgtcacgta gatcaaccgg ggcgcggcgc cgtcccgcgg cgcgcgcagg
    11221 atcatcccct cctgatcgac cgggagcggt gtgagtctcg tccccattgc tgccagtgcc
    11281 gtctgtgccc cgcgataccc aggctcctcg agccacacga cgtcgcccgg gtcggtcagc
    11341 aacgtggcca cgagattgag cgcctgctgc gagctggtga gcacgagcac ctgctcggga
    11401 ctgcagcgca cgccacgcga ttgcgcgaga tagcgggcga tggccgcccg cagcgcggat
    11461 tctccagccg ggtcgccgta gcccagcgcc gcacgagccc cccgcagccg ccgtgcggtg
    11521 aggcgccgcc agggatcgag cggaaactcg gtgaggtccg ggaaaccggc ggaaaatgcc
    11581 atcgcgctcg tgggatcgac gcagccaccg ccgcgcacga tgcggccgcc gcgggcggac
    11641 cacgacgccg tctccagccg acgccggacg gcaggcttga cggcaggcgg atggggaccg
    11701 acgtccacgc tcacgaaggt gccgtcgccc acccgccgcc ggagataacc ctcggcctcg
    11761 agttgggcgt aggcggcctc gaccgtgatg cgggagaccg tgagatcccg cgccatgatc
    11821 cgggaggcgg gcagccgctc gccgtggccc acgcgtccgg attgcacggc gtcccggacg
    11881 gccgcgcaga tccgatcccg cagcgagcgc gccggcccgg ggtgcgactg gaacaacgcg
    11941 aaccagacgg gcgtgcggcg gccgcgctcc gcgattggtc ttatatcgtg ttccataatc
    12001 gcctaatgga taatgccaga cggcgagcta ggctagccgc agacggacgc acgaccctgg
    12061 tcgcgaggcg cagccatgtc ggtcaagaga ctgctcatcc tcctgctcac cggatcgcgg
    12121 accctgacgg gccagactcc gcagctggtg gacatcgggc cgctccggct cgacgtcgtg
    12181 cgcgccggcg cgggagcccc ggcggtggtg ttcgaggccg gcctgggcga tcccctgaac
    12241 gactgggacc gggtgtggcc ggcggtcgcc gaattcacga ccgtcgtgac ctactcccga
    12301 gccggcatcg gccgctccga tccggggcag gaggagcaca gcgcacgccg cgcggcggcc
    12361 gagctgcatg ccctgctcgc gcggctgcgg atcccaccgc cctacgtgct ggtcgggcgg
    12421 tcctacggcg gcatcctggt gcgtctgtac acgtcgctct accccgccga cgtggccggg
    12481 ctggtcctcg tggacggcag ccacgagcag caggtcaaac ggtggggcat gctcgacagc
    12541 acctatcccg gcgcgtttcg cgcattcttc gactccctgc tcggcacgct gcaggccggc
    12601 gcggaagccg cggagatcca ggagtccatg cgcattcagg ccgccgcagg aatcgaaggc
    12661 ctgcgccccc tgcccgacat tcccctggcg gtgctcacca gcatgcaggt gcctgacagc
    12721 cccagcaccg tgaatcagac cgcccggggc cacgaggcct ggcgggcgat gcacgacgag
    12781 tggttccgcc gctcgcgcaa cgcgatccac atcgtgacct ggcgcagcgg gcacgcgatc
    12841 caggacgcgg agccgcagct cgtggtcgac gcgatccggt tcgtgctgga ccgggtgcgc
    12901 agccaatgac gatgcgcttc cctctcgatc aggagtcgcc catggacgag acccgctcgc
    12961 tcgtgctcga atcccccgcg cccgctcccg cggcgagtcg tgatttcatg gcggcgaagc
    13021 tcgccttcca taccgacgcc tgggatgtgg cccagga
//
LOCUS       DBNGZW010000431         3086 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_711466, whole genome shotgun sequence.
ACCESSION   DBNGZW010000431 DBNGZW010000000
VERSION     DBNGZW010000431.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_711466"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..857
                     /locus_tag="AC16JC_10190"
     CDS             <1..857
                     /locus_tag="AC16JC_10190"
                     /inference="COORDINATES: protein motif:HMM:NF014826.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR5165103.1"
                     /translation="AAFADEVSVYRAAGLPWITPELREGLGEIAAASEGRLPPLVEEG
                     DLKGFLHCHSNYSDGSTTIAQWSTAAREAGYEWVGITDHSGSAAYAGGLKAGDVPRQH
                     DEIDAVNRRGDGCRVLKGVEADILADGSLDYGPDVLDRFDFVIGSIHTRFGMTADEMT
                     ERLLRAMEDPRLTILGHPTGRLLLARDPYPLDLDRVLAAAAERGVAVEVNADPHRLDL
                     DWRALSRARELGVTVSIGADAHSTAGMANVPIGIGVARKGGLTAPDVLNARDAAGMLA
                     FARQRRRA"
     gene            854..1543
                     /gene="nth"
                     /locus_tag="AC16JC_10195"
     CDS             854..1543
                     /gene="nth"
                     /locus_tag="AC16JC_10195"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HIR5165104.1"
                     /translation="MKRPRRSIKRKSASRAENPAERRARTRKIVARLRREFPEAKCAL
                     DHASPLQLLVATILSAQCTDVRVNMVTPALFAKYHTAADYAAADPEQFQREIQSTGFF
                     RSKTKSILGMAQALVERHGGRVPDTMDALVALPGVGRKTANVVLGNAFGKNEGIVVDT
                     HVQRISGLLKLTRETTPEKIERDLMELVPRKDWTIFSHLLILHGRKTCIARRPKCESC
                     GISELCPSSRV"
     gene            1548..2696
                     /locus_tag="AC16JC_10200"
     CDS             1548..2696
                     /locus_tag="AC16JC_10200"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011791764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20 metallopeptidase family protein"
                     /protein_id="HIR5165105.1"
                     /translation="MNLPPVDEPSLVHDRRDLHQHPELGFQETRTATLAAERLRGLGY
                     DVHTGVGRTGVLGVRGTGGRCVLLRADMDALPVEEANDVPYRSRHAGRMHACGHDGHV
                     AIGLETARRLAAVALPGTVKIAFQPAEELSGGATAMIDDGVLAAPQVGAALGLHLWNE
                     LPVGTIGLMPGPVMASVDEFDITITGTGGHAAMPHLAIDPVLVAAHVVTALQSLVSRR
                     RDPFEAGVVSVTQLSAGHAFNVIPAEARLLGTVRTFGGRFYEDAPRLVEDTARGLAAA
                     FGATAEVRFRRLTGPLVNDPGITDLMSGVAEEIVGKEHVKHGVRTMGGEDMSRFLERV
                     PGCFAFVGSGRGDGTSASHHSPRFDIEEPALAIGAELLSRTAVRYLAG"
     gene            2720..>3086
                     /locus_tag="AC16JC_10205"
     CDS             2720..>3086
                     /locus_tag="AC16JC_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010879233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HIR5165106.1"
                     /translation="MPNLLAAEPSAYLKSAAHQPVHWFPWGDAAFARAVEDDKPVLLD
                     IGAVWCHWCHVMDSESYEDAAVAEILNRDFICIKVDRDERPDVDARYQRAVQALTGQG
                     GWPLTAFLTPAGEVFFGGTY"
BASE COUNT          469 a         1098 c         1075 g          444 t
ORIGIN      
        1 acgccgcctt cgccgacgaa gtgagcgtct atcgtgcggc ggggctcccc tggatcacgc
       61 cggagctgcg cgaggggctg ggcgagatcg ccgccgcctc ggaggggcgg ctgccgccac
      121 tcgtggagga gggggatctc aagggcttcc tccattgcca ctccaactac tccgacggct
      181 ccacgaccat cgcgcagtgg tccaccgcgg cgcgggaggc gggctacgag tgggtcggga
      241 tcaccgacca ctcgggctcg gctgcctacg cgggcggtct caaggccggc gacgtccccc
      301 gccagcacga cgagatcgac gcggtgaacc gtcgcggcga cggctgccgc gtcctcaagg
      361 gcgtcgaggc ggacatcctg gccgacggca gcctcgacta cggccccgac gtcctggacc
      421 gcttcgattt cgtgatcggc tccatccaca cccgtttcgg catgacggcc gacgagatga
      481 ccgagcgcct gctccgcgcc atggaggatc cacgcctcac gatcctcggc cacccgaccg
      541 gacgcttgct gctcgcgcgc gacccttacc cgctcgacct ggaccgcgtg ctcgccgctg
      601 ccgccgagcg gggggtcgcc gtcgaggtca acgccgatcc gcaccggctc gatctcgact
      661 ggcgcgcgtt gagccgcgcc cgggagctgg gggtgacggt ctcgatcggc gccgacgcgc
      721 attccacggc cgggatggcg aacgtcccca tcgggatcgg cgtggcccgc aaaggcgggc
      781 tcaccgcgcc ggatgtgctc aacgcgcgcg acgcggcggg gatgctggcg ttcgcccggc
      841 agcgacgccg cgcatgaagc gtccgcggcg ctctataaag aggaagagtg cttcccgggc
      901 ggagaacccg gccgagcgcc gggcacgtac ccgcaagatc gtcgcccgcc tgagacggga
      961 gtttcccgag gcgaagtgcg cgctcgacca cgcgagcccg ctgcagctcc tcgtcgccac
     1021 gattctctcg gcccagtgca ccgacgtgcg ggtcaacatg gtcactccgg ccctgttcgc
     1081 gaaataccac acggccgccg attacgccgc ggcggatccc gagcaattcc agcgggagat
     1141 ccagagcacg ggcttcttcc ggagcaagac caagtcgatc ctcgggatgg cgcaggccct
     1201 ggtggagcgc cacggcggcc gggtcccgga cacgatggac gcgctggtgg ccctgcccgg
     1261 cgtcggccgc aagacggcca acgtcgtcct gggaaacgcc ttcggcaaga acgaaggcat
     1321 tgtggtcgat acccacgtgc agcgcatcag cggattgctc aagctcaccc gcgaaaccac
     1381 tcccgagaag atcgagcgcg acctgatgga actcgtcccc cggaaggact ggacgatctt
     1441 ttcgcacctg ttgattctcc acggccgcaa gacgtgcatc gcgcgccggc cgaagtgtga
     1501 aagctgcggg atcagcgaac tctgtccgag ctcgcgagtc tgagtccatg aaccttcccc
     1561 cggtagacga accctcgctc gtccacgacc gccgcgacct gcaccagcac cccgagctgg
     1621 ggttccagga aacgcgcacc gcgacgctcg ccgccgagcg gctgcgcggt ctcggatacg
     1681 acgtgcacac gggcgtgggg cgcaccgggg tactcggcgt ccgggggacg ggcgggcggt
     1741 gcgtgctgct gcgcgccgac atggacgcgc tccccgtgga ggaagcgaac gacgtcccct
     1801 accgctcgcg ccacgcgggc cgcatgcacg cgtgcgggca cgatgggcac gtggcgatcg
     1861 gactcgagac ggcgcggcgt ctggccgcgg tggcgctccc ggggacggtg aagatcgcgt
     1921 tccagccggc cgaagagctc tcgggcggag cgacggcaat gatcgacgac ggcgtgctcg
     1981 cggcccccca ggtgggcgcc gccctcggcc tgcacctctg gaacgagctg ccggtgggga
     2041 cgatcggcct catgccgggt ccggtcatgg cctcggtgga cgagttcgac atcacgatca
     2101 ccggcacagg cgggcacgcc gcgatgccgc atctcgcgat cgacccggtg ctcgtcgccg
     2161 cccacgtcgt caccgcgctg cagagtctcg tgtcgcgtcg ccgcgatccc ttcgaggcgg
     2221 gcgtcgtgtc ggtcacccag ctgtccgccg gtcacgcctt caacgtcatt cccgccgagg
     2281 cgaggctgct cggaacggtg cgcaccttcg gcgggcgctt ctacgaggac gcgccgcggc
     2341 tcgtcgaaga cacggcgcgc gggctggcgg cggcgttcgg cgccacggcc gaggtccgct
     2401 tccgccggct caccggcccg ctcgtgaacg accccgggat cacggacttg atgagcggcg
     2461 tggccgagga gatcgtcggg aaggagcatg tgaaacacgg cgtccgcacg atgggcggcg
     2521 aggacatgag ccggttcctc gagcgtgtcc cgggctgctt cgccttcgtg ggatcgggac
     2581 ggggcgacgg cacctccgcg tcccatcaca gtccgcgctt cgacatcgag gaacccgccc
     2641 tggccatcgg cgccgagctg ttgagccgga ccgcggtgcg gtacctcgcg ggatgagccg
     2701 tgagtagatt ccgccgtaca tgcccaacct gctcgccgcc gagccctccg cctacctgaa
     2761 gtccgcggca catcagccgg ttcactggtt cccctggggt gatgcggcgt tcgcgcgggc
     2821 cgtcgaagac gacaagcccg tcctgctgga catcggcgcc gtgtggtgtc actggtgcca
     2881 cgtgatggac agcgagtcct acgaagacgc cgcggtggcc gagatcctga accgcgactt
     2941 catctgcatc aaggtcgatc gcgacgagcg ccccgacgtg gacgcgcgct accagcgcgc
     3001 cgtgcaggcg ctcaccggcc aggggggctg gccgctcacc gcgttcctga ccccggccgg
     3061 cgaagtgttc ttcggcggca cgtact
//
LOCUS       DBNGZW010000432         3832 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_177370, whole genome shotgun sequence.
ACCESSION   DBNGZW010000432 DBNGZW010000000
VERSION     DBNGZW010000432.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_177370"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..174
                     /locus_tag="AC16JC_10210"
     CDS             4..174
                     /locus_tag="AC16JC_10210"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5165107.1"
                     /translation="MGSHGKGVVERMLVGSVTERLLGHLPAPMLLVVPVARLAPTPHP
                     ATTAKLRMATVA"
     gene            complement(233..1615)
                     /locus_tag="AC16JC_10215"
     CDS             complement(233..1615)
                     /locus_tag="AC16JC_10215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020469055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HIR5165108.1"
                     /translation="MTDTIQFLGAAGTVTGSMHLVTVEKRRVLLDCGLFQGLKELRLR
                     NWREPPFDPKAIDAVVISHAHIDHTGSLPLLVRRGYRGPIFCTPATADLLGVLLPDSA
                     HLQEEEAERANRYGYTRHKPALPLYTAADAERALGQLERTGYDRPFPVAGDVSVTYRT
                     AGHILGAATVDLQLGAGQRLVYSGDLGRYDRPILPDPAPVPAADVLLLESTYGDRQHP
                     TGTADELAKVVNETAERGGALLVPAFVVDRTQEVLWLLQHLEEERRIPVLPVYVDSPS
                     ATEVTEIYRRHVEEQDGEMRSRTQDGKDPLKPGRLQFCRTMEESKRLNDIRGPVIIVS
                     ASGMATGGRILHHLQHRLPDPRTTVLLVGFQAAGTRGRSLQDGARTLKMFGEQVPVRA
                     RVETVHGLSAHADQAELLRWLGGFTRAPRTTYLVHGEPEAARAMAQAITARYGWAVSI
                     AQDGELVALT"
     gene            complement(1612..2307)
                     /locus_tag="AC16JC_10220"
     CDS             complement(1612..2307)
                     /locus_tag="AC16JC_10220"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009509390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HIR5165109.1"
                     /translation="MLDLIRNRLRQGIRTLTFPKGPAPDLPERFRGRPTQDPATSPRG
                     SAGLDLGTCLFSPEEAATYTRDYRMAVARREDLVTPTGEVELARALDERSRRLFGRSL
                     RLRSVVAGSCGGCEAELVALGNVVFDLARFGVQFVASPRHADGLVVTGAVNVNMQTAL
                     RKTYDAVPAPKIVLAVGACALSGGPFRGSPEVGHGVEGVPVDLWVPGCPPHPLTILDG
                     ILRLLGAIEGGSR"
     gene            complement(2315..>3832)
                     /locus_tag="AC16JC_10225"
     CDS             complement(2315..>3832)
                     /locus_tag="AC16JC_10225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase large subunit"
                     /protein_id="HIR5165110.1"
                     /translation="VTAFQELGLRNRRAIPWGSVPLESLDGFRDTLVRATARGWRLAS
                     LFGLPDSGGDQQTRLVAILADDIGGELGATSARVEQRYAALTPVCPQANRFEREIAEQ
                     CGVLPEGHPWLKPLRRHPPDHAPRGRRTAAADREAYPFFQVEGEEVHEVGVGPVHAGI
                     IEPGHFRFQAHGEAVLFLEIVLGYQHRGVERLLETLEADRGVLVAESIAGDTVIGHVG
                     AYCGVLEALARSRKTPRAQTIRGIALEIERLANHIGDLGALAGDVAYAPAAAYLGRLR
                     GECLNLLMTLSGNRYGRGLVRPGGAGFDIPSEMGREMRDRMQHLRHELEPVLDLLFRS
                     ASVQARFEGVGVVTKDACIEEGYVGPVARACEVARDVRHDHPYGIFRFAHIPVATAWA
                     GDVLGRALVRRLEIERSLEFVIEQLGALPKGEARVPCGPLRPDELAVAMVEGWRGEIV
                     HVVITDHDGKVRRHKVTDPSFHNWAALARAMPGNEISDFPLCNKSFNLSYAGHDL"
BASE COUNT          572 a         1425 c         1291 g          544 t
ORIGIN      
        1 gtcgtgggct cgcacggcaa gggggtcgtg gagcggatgc tcgtcggcag cgtcaccgag
       61 cggctgctcg gccaccttcc ggcgccgatg ttgctcgtcg tcccggtcgc cagactggcg
      121 ccgacgccgc acccagcgac cacggcaaag ttgaggatgg ccaccgtggc ctaggagggg
      181 gagtcgcgtc gttgcacggg gcccggcggc aacgccgggc cccgttaccg tatcaggtga
      241 gggcgaccag ctccccgtcc tgcgcgatgc tcaccgccca gccgtagcgg gcggtgatcg
      301 cctgcgccat cgcccgggcc gcctccggct cgccgtggac gaggtacgtg gtccggggcg
      361 cgcgcgtgaa acccccgagc cagcgcagca gctccgcctg atccgcgtgg gccgagagac
      421 cgtggaccgt ctcgacgcgc gcgcgcaccg gcacttgctc gccgaacatc ttgagcgtcc
      481 gcgcgccgtc ctgcaacgac cgtccgcgcg tgcccgccgc ctgaaacccc accagcagca
      541 cggtcgtgcg cgggtccggc aagcgatgct gcagatggtg cagaatccgc ccgccggtgg
      601 ccatgcccga agccgacacg atgatgacgg gcccgcgaat gtcgttcagc cgcttcgact
      661 cctccatggt gcggcagaac tggaggcggc cgggcttgag cggatccttt ccatcctgcg
      721 tgcggctccg catctcgccg tcctgctcct cgacgtgacg acggtagatc tccgtgacct
      781 ccgtggccga tgggctgtcc acgtacaccg ggagaacggg gatgcgacgc tcctcttcga
      841 gatgctgcag cagccagagc acctcctgcg tccggtccac cacgaacgcc gggaccagca
      901 acgcacctcc tcgctcggcg gtctcgttga ctactttcgc cagctcgtcc gccgttcccg
      961 tcgggtgctg gcgatcgccg tatgtggatt cgagcagcag cacatcggcg gcgggtaccg
     1021 gcgcgggatc gggcagaatg ggccggtcgt agcgccccag gtcgccggag tagaccagcc
     1081 gctgccccgc gccgagctgc agatccacgg tcgcggctcc caggatgtgc cccgcggtcc
     1141 ggtaggtgac ggacacatct ccggcgaccg gaaacggccg gtcatacccg gtccgctcga
     1201 gctggcccag cgcccgctcc gcgtccgcgg cggtgtagag cggcagcgcc ggcttgtgcc
     1261 tggtgtagcc gtagcggttg gcgcgctccg cctcctcctc ctggaggtgg gccgagtccg
     1321 gcaacagcac gcccaggaga tcggccgtgg cgggggtgca gaagatcggc ccgcggtagc
     1381 cgcgccgcac cagcaggggg agcgatccgg tgtggtcgat gtgcgcgtgc gagatcacca
     1441 cggcgtcgat cgccttggga tcgaagggcg gctcgcgcca gttgcgcaac cgcagctcct
     1501 tcaagccctg gaacaggccg caatcgagca gtacccggcg tttctcgacg gtcaccaggt
     1561 gcatcgagcc ggtcactgtc ccggcggcgc cgaggaattg gatcgtgtcg gtcatcgcga
     1621 cccgccctcg attgcgccga gcagccgcag gatgccgtcc aggatcgtga gtggatgcgg
     1681 tgggcacccc ggcacccaca ggtccaccgg cacgccctcc acaccgtggc cgacctccgg
     1741 gctgccgcga aatggcccgc cgctcagagc acaggcgccc accgcgagga cgatcttcgg
     1801 cgctggaacg gcgtcgtagg tctttcgcaa tgccgtctgc atgttgacgt tcacggcgcc
     1861 ggtgacgacc aacccatcgg cgtgacgcgg cgacgcgacg aactgcacgc cgaagcgtgc
     1921 caggtcgaac accacattgc ccagcgcaac gagctccgct tcgcaaccac cgcagctgcc
     1981 cgcaacgacc gatcgcagcc gcagcgagcg tccgaacagg cggcggctcc gctcgtcgag
     2041 ggcgcgggcc agctccacct cacccgtcgg cgtcaccagg tcctcccggc gcgcgacggc
     2101 catgcgatag tcgcgcgtgt acgtcgccgc ttcctcggga ctgaagagac acgtcccgag
     2161 atccagcccg gcggagccgc gcgggctcgt ggcggggtcc tgtgtcgggc gccctcggaa
     2221 ccgctcggga aggtctggcg ccgggccctt cgggaacgtc agcgtgcgga tcccctgccg
     2281 caaccgatta cggatcagat cgagcacggc aacctcacag gtcgtgcccc gcgtaggaca
     2341 ggttgaagct cttgttgcac agcgggaaat cggagatctc gtttcccggc atggcgcggg
     2401 cgagcgcggc ccagttgtgg aacgacggat cggtcacctt gtggcggcgc accttcccgt
     2461 cgtggtcggt aatcacgacg tggacgatct ccccccgcca cccctcgacc atcgccacgg
     2521 ccagctcgtc gggccggagc ggtccgcagg gcacgcgtgc ctcgcccttg ggcagcgcgc
     2581 ccagctgctc gatgacgaat tccagggacc gctcgatctc cagccggcgg accagcgccc
     2641 gcccgagcac gtcgccggcc caggccgtgg ccaccgggat gtgcgcgaag cggaagatgc
     2701 cgtaggggtg atcatgccgc acgtcgcgcg cgacctcgca cgcccgcgcc accggaccca
     2761 catagccctc ctcgatgcac gcgtccttgg tcaccacgcc cacgccctcg aaccgcgcct
     2821 ggaccgaagc gctgcggaac aacaggtcga gcaccggctc cagctcgtgg cgcaggtgct
     2881 gcatccggtc gcgcatctcc cgccccatct ctgacgggat gtcgaaaccc gcgccgccgg
     2941 gtcgcaccag cccgcgcccg taccggttgc cgctcagcgt catcaggaga ttgagacact
     3001 ccccgcgcag ccggcccagg tacgccgccg ccggcgcgta ggcgacgtcc cccgcgagcg
     3061 cccccaggtc gccgatgtga ttcgcgagcc gttcgatctc gagagcgatg ccgcggatcg
     3121 tctgcgcgcg cggcgtcttg cggctgcgcg ccagcgcctc gaggacaccg cagtaggcgc
     3181 cgacatgtcc gatgaccgtg tcgccggcga tcgactccgc caccaggacg ccgcggtcgg
     3241 cctcgagcgt ctccagcagc cgctccacac cgcgatgctg gtaccccagc acgatttcca
     3301 ggaacagcac cgcctccccg tgcgcctgga aacggaagtg ccccggctcg atgatcccgg
     3361 cgtgcacggg ccccaccccc acctcgtgca cctcctcgcc ttccacctgg aagaacggat
     3421 aggcttcccg gtctgccgct gccgtgcgcc ggccgcgcgg cgcgtgatcg ggtgggtggc
     3481 gccgcaacgg cttgagccag ggatgacctt cgggaagcac gccgcactgc tcggcgatct
     3541 cgcgctcgaa gcggttggcc tgcggacaca ccggcgtgag cgccgcatag cgctgctcca
     3601 cccgcgcgct cgtggcgccc agctcgccgc cgatgtcgtc ggccaggatc gcgacgagac
     3661 gggtctgctg gtccccgcca ctatcgggca ggccgaagag cgaggcgagc cgccagcccc
     3721 tggccgtcgc ccggaccaac gtgtcgcgga agccgtccag cgactcgagc ggcaccgatc
     3781 cccacgggat ggcgcgccgg ttccgcagcc ccagctcctg gaacgcggtc ac
//
LOCUS       DBNGZW010000433         7594 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1472529, whole genome shotgun sequence.
ACCESSION   DBNGZW010000433 DBNGZW010000000
VERSION     DBNGZW010000433.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7594)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7594)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7594
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1472529"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(21..686)
                     /locus_tag="AC16JC_10230"
     CDS             complement(21..686)
                     /locus_tag="AC16JC_10230"
                     /inference="COORDINATES: protein motif:HMM:NF014420.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HIR5165111.1"
                     /translation="MRTLHPFTLIGIALALPALAWLLPSAGGVLAPMSLALALCAAAG
                     GTGFGVLRAALLTAAPFWFFLFLLHGVAGMLTTAPRLTTMIASFLWLVAVLPPARLVE
                     AMVAAGWPASAAFLLSATLSAVPALRERARRIVDAQRCRGLPTRGGPLTRLAALRALA
                     LPLVLSALHEVDERALALETRGFSGSLRRTSLAPPPDPAVERVLRWGLLVGCLGLLAW
                     RLL"
     gene            complement(687..1262)
                     /locus_tag="AC16JC_10235"
     CDS             complement(687..1262)
                     /locus_tag="AC16JC_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165112.1"
                     /translation="MSVRLLALIPVAVAINLALSAAVDAVGLPLFLDTTGTILATALA
                     GPVVGIATGALTSAVNSLRNPTFWPFVVVQVLVALYAWGAARAGWFRSLAVAVPAGLA
                     LGVLAAVTSTPISYLIFKCATTGGIALTTKLGQLLGLSDLGACFFGSFLSDFADKAVT
                     FALVTTVLHALPRRTAARFPLALRAIGRSAA"
     gene            1307..2581
                     /locus_tag="AC16JC_10240"
     CDS             1307..2581
                     /locus_tag="AC16JC_10240"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR5165113.1"
                     /translation="MHITSLLLATRLVLQTQAHDTLTPAAHRALNDLHYLADDAREGR
                     GVGTRGLQDAGAYLARAFKTIGLAPGGDSGTYFQTFTISPTAPAVAHSDAGGKTIRNV
                     IGVLRGRSPARRGEVIVVGAHYDHLGLGGFGALDDPDSTGKVHNGADDNASGTTALLE
                     VARRLGRRGGPDRTVLFIAFSGEELGDLGSGHYVAHPTVPLDSIYAMLNMDMVGRLRN
                     AKLIVSGAATTKEFPALLDSLNRAGGSGPRFDLRASGDGWGPSDHASFYAAKRPVLHF
                     FTDLHGDYHRTTDDWDKINIVGLEEVSDFVAATVTALADRPGALTFVDAPPPRTAVGG
                     AGYGAYLGTIPDMSESPGGVRITGARAGSPAEQAGLKAGDVITAIGAKTVANLYDMTD
                     ALRSHQAGDTVVIVVKRDSTTMRLTAILGKRS"
     gene            2578..3042
                     /locus_tag="AC16JC_10245"
     CDS             2578..3042
                     /locus_tag="AC16JC_10245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5165114.1"
                     /translation="MTAGLNHDPTAVVRALRAAHLHDLPRQGLLQLACEKIQAWGGPY
                     TSVYAYMLHGDELVLEAHAGRDTDHVRIPVGAGVCGTAVARKEDQNVPDVRAVGNYLA
                     CNLYTRSELVVLIRRGDHILGQIDIDSDVLNGFGEREHADVKAVAEALAVLL"
     gene            3120..3416
                     /locus_tag="AC16JC_10250"
     CDS             3120..3416
                     /locus_tag="AC16JC_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010495659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR5165115.1"
                     /translation="MTTSKLLGPFCQSCSMPLNKPEDFGTDIVGYRVNDYCRHCYANG
                     AFTEPHITLQEMTDKCVDVMVRQGIMPEAKARTLMMEVLPRMKRWRLVTASAAV"
     gene            complement(3394..4155)
                     /locus_tag="AC16JC_10255"
     CDS             complement(3394..4155)
                     /locus_tag="AC16JC_10255"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5165116.1"
                     /translation="MMRQALLAPMVMAEALTGQGTTVDTIRIAGQPLLVHRYAPAGGS
                     RCAPVLLLSGDGGWVLGVVQWAETLQADGHEVVGLDAARLVKLVGAAGLAGLVTDWPE
                     LARLTRTPPVVLGYSRGATLGLVLASRAGSPPPVVLLGVDLEDHFDGPAVPGGLSPAL
                     RHRGEYVVDLRPLFVDSARTTRVAIVHGLRDRVVPYDAVRPWFDRLSEPKRISILPHS
                     GHGFGDSRTVLPAIRESLDWAAGEACRTLRPPRSR"
     gene            complement(4152..4886)
                     /locus_tag="AC16JC_10260"
     CDS             complement(4152..4886)
                     /locus_tag="AC16JC_10260"
                     /inference="COORDINATES: protein motif:HMM:NF017835.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AcvB/VirJ family lysyl-phosphatidylglycerol
                     hydrolase"
                     /protein_id="HIR5165117.1"
                     /translation="MMSYARVVLLAGLLASTVLRGVPSQEDPLNRLPVHRYVPADRPV
                     GGVVFFSGDGGWRSFDKANADSLRTLGYWVLGVDDLHLFAKEMPGDTLGAVGRRLVTY
                     LRGQLPEGAPVYLAGYSFGASIVADAAARGVPSDGLYLLGPGVRGIRKITVEGFLDRD
                     PAGSTSFDVAERLNLRGCVPVVFVTGESDKAGKASQVYPRVHQPVQQFLVTGASHHYY
                     GGDARYMAVARQALDWLQHHRRECAP"
     gene            complement(4883..5275)
                     /locus_tag="AC16JC_10265"
     CDS             complement(4883..5275)
                     /locus_tag="AC16JC_10265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165118.1"
                     /translation="MNNRWIYGLELAVFGWISARGGWFAVRSLTPFGRVVGALALAFA
                     AATAALFGAFALNRPIRPLLHLTPFIGAGLVFLHETMRAARTVPGWTAPFVGLYSTLA
                     MAVARRTPAVPHEVSQPELSEGDEENEG"
     gene            complement(5272..6594)
                     /locus_tag="AC16JC_10270"
     CDS             complement(5272..6594)
                     /locus_tag="AC16JC_10270"
                     /inference="COORDINATES: protein motif:HMM:NF021445.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HIR5165119.1"
                     /translation="MVSTHDTRPALDLPPGEASPAEVRAVLQRYGSNADSFQAELSYH
                     RRLYFGEEGVVACARIGRVTVVAGDPVAPPGRLLPLVAALRSALPGPVAMVSASEQVR
                     DELAADGWGWVKVGEEPYWEPGRWSLDGKAAANVRHAVNAARRRDLRVEQTGPGQPGW
                     NEDLTAMRAVAEAWSGTHETRPLGFLLTLAPFERPEDRRYFVAREPSGRIVAFLAAAP
                     IWARDGWYFQDFVRLPDAPNGVNELLFHEAMRAMQAAGARLVTLGAAPLAGLDKEEPQ
                     RPWIHRVLRFAYEHLDAFYHFQTLSDYKAKYAPHWWEPKYLVFHPRRLRARVLFAVLK
                     AYDPRGATRLVLSRLAEGLREELAIDPDTGRPRVVGHALRLPGLLLQQDLLVAVALAL
                     AIGSGIGIVADHLRRSFDDVYVATLSSLIAASLTVALLHARARHEANP"
     gene            complement(6560..7114)
                     /locus_tag="AC16JC_10275"
     CDS             complement(6560..7114)
                     /locus_tag="AC16JC_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR5165120.1"
                     /translation="MGGLLLYLGLAVTVMFQEEAAPIAGALAAHHGHGRLWLVILACM
                     TGTYVGDLVLYALGRRTRTLIARPRFGRALAFIRRHPRGAPLAVRFAYGLRFTLPVTC
                     GAAGIPFGTYALWVGVSAALWATGFGLAGWGAGELAVRLFHDAKHHEVPMIAIVLLVW
                     LIVFVVLHLRGRHHGVDARHAAGA"
     gene            7281..>7594
                     /locus_tag="AC16JC_10280"
     CDS             7281..>7594
                     /locus_tag="AC16JC_10280"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5165121.1"
                     /translation="MNLRVNGVDLFTRRIGTGPLVVVLHGGPGAHHDYLLPQFDRLSA
                     RRELFYYAQRGGGRSPVARDVPVGWQQHVADLEAVRELLGLERLTLCGYSWGGLLAVL
                     YLL"
BASE COUNT         1292 a         2704 c         2620 g          978 t
ORIGIN      
        1 gccggcgagc gtgagcgcct tcacaggagc cgccacgcga gcagtccaag gcagccgacc
       61 agcagccccc aacgaagcac ccgctccacg gccggatcgg gcggcggggc gagcgacgtg
      121 cggcgcaacg accccgagaa gccgcgcgtc tcgagcgcga gggcgcgctc gtcgacttcg
      181 tgcagcgccg agagcacgag cgggagcgcg agggcgcgca gcgccgcgag gcgtgtcagc
      241 ggtcctcccc gcgtgggcag tccgcggcag cgctgcgcgt ccacgatgcg gcgcgcccgc
      301 tcgcgcagcg cgggaacggc ggagagcgtg gccgagagca ggaacgcggc gctcgcaggc
      361 catccggcgg cgaccatcgc ctccaccagc cgggcgggag gcaggacggc gacgagccag
      421 aggaagctcg cgatcatcgt ggtgagccgc ggcgcggtcg tcagcatgcc cgcgacgccg
      481 tgcagcagaa agaggaagaa ccagaacggg gcggcggtca gcagcgcggc gcgcaacacg
      541 ccgaagcccg ttcccccggc ggccgcgcac agcgccaggg ccagcgacat gggagcaaga
      601 acgcccccgg cggacgggag cagccaggcg agcgcgggca gggccagcgc gatgccgatc
      661 agcgtgaacg gatggagcgt ccgcacctag gcagcggagc ggccgatggc gcgcaaggcg
      721 agcgggaagc gggcggcggt gcgccgcggc agcgcgtgca gcaccgtggt cacgagggcg
      781 aaggtcacgg ccttgtcggc gaaatccgag agaaagctcc cgaagaagca ggcgccgaga
      841 tccgacagcc cgagcagctg gcccagcttg gtggtgagcg cgatgccgcc cgtggtggcg
      901 cacttgaaga tgaggtagga gatcggcgtg gacgtcacgg ccgcgagcac gcccagtgcg
      961 agacccgccg gaaccgcgac tgcgagcgac cggaaccatc cggcccgcgc agcgccccag
     1021 gcgtacagcg cgaccagcac ctgcaccacc acgaacggcc agaacgtggg gttccgcagg
     1081 ctgttcaccg ccgaggtgag ggctccggtg gcgatcccga cgaccggtcc ggcgagcgcc
     1141 gtcgcgagga tggttccggt ggtatccagg aacagcggca gccccaccgc gtctaccgca
     1201 gcggacaggg cgagattgat cgcgacggcg acggggatca gcgcgagcag gcgaaccgac
     1261 accgcatgac ttccgtgaac agaggcgcaa agatagatat tctggcatgc acatcaccag
     1321 cctgctcttg gccacgcgcc tcgtgctcca gacccaggcg cacgacacgc tgaccccggc
     1381 ggcccaccgg gcgctgaacg atctccacta tctcgccgac gacgcccgcg aggggcgcgg
     1441 cgtgggaacg cgtggtctgc aggatgccgg cgcctacctg gcccgggcgt tcaagaccat
     1501 cggtctcgcc ccgggtggcg attccggaac ctacttccag acgttcacga tctcgcccac
     1561 ggctccggcc gtcgcgcaca gcgatgccgg gggcaagacg atccgcaacg tcatcggggt
     1621 gttgcgcggc cgctccccgg cacggcgcgg cgaggtcatc gtcgtgggcg cgcactacga
     1681 tcatctgggc ctcggcggct tcggcgcgct ggacgatccg gactcgaccg gcaaggtgca
     1741 caacggcgcg gacgacaacg cttcgggcac gaccgccctg ctcgaggtag cgcgccggct
     1801 cggccgccgc ggcgggcccg accgcaccgt gctgttcatc gccttctcgg gcgaggagct
     1861 gggtgacctg ggctcggggc actacgtcgc ccatcccacc gtccccctgg attcgatcta
     1921 cgcgatgctc aacatggaca tggtgggacg gttgcggaac gcaaagctca tcgtctcggg
     1981 cgcggcgacc accaaggagt tccccgccct gctcgactcc ctcaatcgcg ccggcgggag
     2041 cggtccgcgg ttcgatttgc gcgcctccgg cgacggctgg gggccgagcg accacgcgtc
     2101 gttctacgcg gcgaaacggc cggtgctcca tttcttcacc gatctgcatg gcgactatca
     2161 ccggaccacc gacgattggg acaagatcaa catcgtcggg ttggaggagg tgtccgattt
     2221 cgtcgccgcc acggtgacgg cgttggccga ccggcccggc gccctgacgt tcgtggacgc
     2281 gccgccgcca cgaactgccg tcggcggcgc cggctacggc gcctacctcg gcaccatccc
     2341 ggacatgagc gaatcgcccg gtggcgtgcg catcacgggc gcccgtgcgg ggagtccggc
     2401 ggaacaggcg ggactcaagg ccggggacgt catcaccgcg atcggtgcga agaccgtcgc
     2461 caacctatac gacatgaccg atgcgctgcg cagccaccag gcgggcgaca ccgtcgtcat
     2521 cgtcgtgaag cgggattcga cgaccatgcg cctgactgcg atcctgggca agcgctcgtg
     2581 accgccgggc tgaaccacga ccccacggcg gtggtccgcg cgctgcgcgc cgcccacctg
     2641 cacgacctcc cgcgacaggg actgttgcag ctggcgtgcg agaagatcca ggcctggggc
     2701 gggccgtaca cctcggtcta cgcctacatg ctgcacggcg acgagctggt gctcgaggcc
     2761 cacgcgggac gcgacaccga ccacgtgcgg atccccgtcg gcgcgggcgt gtgcggtacc
     2821 gccgtggcgc ggaaagagga ccagaacgtc cccgacgtcc gcgcggtcgg caactacctc
     2881 gcgtgcaacc tctacacccg gtccgagctg gtcgtgctga tccgccgcgg cgaccacatc
     2941 ctgggccaga tcgacatcga ctccgatgtg ctgaacgggt tcggggagcg cgagcacgcc
     3001 gacgtcaagg ccgtcgccga ggcgctggcc gtcctgctct agcagtcctc ttcatggctc
     3061 cttcagcgtc gcctcatcgc cccgtgcgag gttttcctca tggaccgaag gagggagcca
     3121 tgacgacgag caagctgctg ggaccgttct gccagagctg cagcatgccg ctcaacaagc
     3181 cggaggattt cggcaccgac atcgtcgggt atcgcgtgaa cgactactgc cgtcactgct
     3241 atgcgaacgg cgcattcacg gagccgcaca tcaccctgca ggaaatgacc gacaagtgcg
     3301 tggacgtcat ggtccggcag ggcatcatgc cggaggccaa ggcgcgcacg ctgatgatgg
     3361 aagtgctgcc gcggatgaag cggtggcgcc tggttaccgc gagcgcggcg gtctgagcgt
     3421 ccggcaggcc tcgccggccg cccaatccag cgattcccgg atcgccggca ggacggtgcg
     3481 cgaatccccg aagccgtggc cgctgtgcgg caggatgctg atgcgcttcg gctcggaaag
     3541 acggtcgaac cacggacgga ccgcgtcgta cggcacgacg cggtcgcgca ggccgtgcac
     3601 gatcgccacc cgtgtcgtcc gcgcactgtc cacgaagagc ggtctcaagt cgacgacgta
     3661 ttcgccacgg tgacggaggg cgggagacag gccgccgggc accgcgggcc cgtcgaagtg
     3721 atcctcgagg tccacgccga gcagcacaac gggcgggggt gacccggccc gtgaggccag
     3781 gacgaggccg agcgtggccc cccggctgta gccgagcacc acgggaggcg tccgggtgag
     3841 acgggccagc tcgggccagt cggtcaccag ccctgcgagc cctgcggcgc ccacgagctt
     3901 cacgagccgg gcggcgtcga gacccacgac ctcatggccg tcggcctgca atgtctccgc
     3961 ccactgcacg acgcccagta cccacccccc gtcccccgat agcagcagga cgggagcgca
     4021 gcgtgacccg ccggccggcg catagcggtg cacgagcagc ggctgtccgg cgatgcgaat
     4081 cgtgtccacc gtcgtcccct ggccggtgag cgcctccgcc atgaccatgg gcgccagcag
     4141 cgcctgccgc atcacggcgc acactcccga cggtggtgct gcaaccagtc gagcgcttgc
     4201 cgggcgacgg ccatgtaccg ggcgtctccg ccgtagtaat ggtggctcgc tccggtgacg
     4261 agaaactgct gcaccggctg gtgcacgcgc gggtacacct gcgaagcttt cccggccttg
     4321 tccgattcgc ccgtgacgaa cacgacggga acacagccgc ggaggttcag ccgctcggcg
     4381 acatcgaagg atgtcgagcc tgcgggatca cggtccagga atccctcgac ggtgatcttg
     4441 cggatgccgc ggaccccagg tcccaggagg tacaggccgt cggacggcac cccgcgagcc
     4501 gccgcgtcgg cgacgatgct cgccccgaag ctgtagccgg cgaggtacac cggagcaccc
     4561 tcgggcaact ggccgcgcag gtaggtcacg agccggcggc cgaccgcccc gagcgtatca
     4621 cccggcatct ccttggcgaa cagatgcaag tcgtccaccc ccaggaccca gtagccgagt
     4681 gttcggagcg aatcggcgtt cgccttgtcg aaggagcgcc agccgccgtc gccggagaag
     4741 aacaccacac ctcccaccgg ccggtccgcg ggaacgtagc ggtggaccgg cagacggttc
     4801 aacggatcct cttgcgacgg caccccgcgg aggactgtcg acgccaggag cccggcgagc
     4861 aacacgacgc gcgcgtagct catcatcctt cgttctcctc atcgccctcg gaaagctccg
     4921 gctgactcac ttcgtgcggc acggccggcg tacgacgtgc cacggccatc gcgagtgtgg
     4981 aatacagccc cacgaacggg gccgtccacc cgggaacggt ccgagcggct ctcatcgtct
     5041 catggagaaa caccagtcct gctccgatga acggcgtcag gtgcagcagc gggcgtatcg
     5101 gccggttcag cgcgaaggcg ccgaacaagg cggcggtcgc ggcggcaaac gcgagtgcga
     5161 gcgccccgac gacgcgcccg aacggagtga ggctgcgcac ggcgaaccag ccgccgcgcg
     5221 cggagatcca gccgaacacg gcgagctcga gcccgtagat ccaccggttg ttcatggatt
     5281 cgcctcatga cgagcacggg catgcaggag cgcgaccgtg agactggcgg cgatcaggct
     5341 cgacagggtg gcgacgtaga cgtcgtcgaa gctgcggcgc aggtgatccg cgacgatccc
     5401 gatgccggac ccgatcgcca gcgccagggc gaccgctacc agcaggtcct gctgcagcaa
     5461 cagcccgggg agccgcagcg cgtgcccgac gacccgcggt cggccggtgt ccggatcgat
     5521 ggcgagctcc tcccgcaacc cttcggcgag tcgcgagagc acgaggcgcg tggcgccgcg
     5581 gggatcgtac gccttcagga cggcgaacaa cacgcgagcc cggaggcgcc gcgggtggaa
     5641 caccaggtac ttcggctccc accagtgcgg ggcgtacttg gctttgtagt ccgacaacgt
     5701 ctggaaatga tagaaggcat ccaggtgctc gtaggcgaag cgcagcacgc ggtggatcca
     5761 ggggcgctgg ggctcctcct tgtcgagccc cgcgagcgga gcagcgccga gcgtgacgag
     5821 gcgcgccccc gccgcctgca tcgcccgcat cgcctcgtgg aacagcaact cgttcacgcc
     5881 gttcggcgcg tcgggaagcc gcacgaaatc ctggaagtac catccgtcgc gcgcccagat
     5941 cggcgccgcc gccaggaatg cgacgatgcg ccccgacggc tcgcgcgcga cgaagtagcg
     6001 ccggtcttcc ggtcgctcga agggtgccag ggtcagcaag aaccccagtg gacgggtctc
     6061 gtgcgtcccg ctccacgcct ccgccacggc ccgcatcgcc gtgagatcct cgttccaacc
     6121 cggctgcccc ggtcccgttt gctcgacccg aagatccctg cggcgcgccg cgttgaccgc
     6181 atggcgcacg ttcgccgcgg cctttccgtc cagcgaccag cgccccggct cccagtacgg
     6241 ctcctccccc accttgaccc atccccagcc gtcggctgcc agctcgtccc ggacctgctc
     6301 gctggcgctc accatcgcca ccggccctgg cagggcggac cgcagcgctg cgaccagggg
     6361 tagcagtcgc cccggcggcg ccaccggatc accggcgacc acggtgacgc ggccgatccg
     6421 ggcgcaggcc acgaccccct cctcgccgaa gtacagccgg cggtgatagg acaactccgc
     6481 ctggaacgaa tcggcattgc tgccgtagcg ctgcagcacg gcgcggacct cggcgggcga
     6541 agcctcgccc ggcggcaagt caagcgccgg ccgcgtgtcg tgcgtcgaca ccatgatggc
     6601 gacctcgaag atgaagaacg acgaacacga tgagccagac gagcaacacg atcgcgatca
     6661 tcggcacctc gtggtgcttc gcgtcgtgga acagccgcac cgcgagctct cccgcgcccc
     6721 acccggccag cccgaacccc gtcgcccaca gcgccgccga cacgcccacc cacagcgcgt
     6781 aggtcccgaa cgggatcccg gccgccccgc acgtgacggg cagcgtgaac cgcagtccgt
     6841 aggcgaaccg taccgcgagc ggggcgccgc gcggatggcg gcggatgaat gcgagcgcgc
     6901 gcccgaaccg cggccgggcg atcagagtgc gcgtccgtcg ccccagcgcg tacaggacca
     6961 gatcgccgac ataggtaccg gtcatgcagg cgaggatgac cagccacagc cggccgtggc
     7021 cgtggtgcgc cgcgagcgca ccggcgatgg gagcggcctc ctcctggaac atgacggtca
     7081 cggccagccc caggtataag agcaacccgc ccatacgaga cagctactca gtcgtcctgt
     7141 ccgatactgg taaggcggca tgaagaatca ctcaagtcgt catgccactc accagataga
     7201 ccgcgtcatg catgctccgg gtcacgaccg gccggggcaa ccccgtgacc ggattccgtc
     7261 acggttatct tgcgtccccc atgaacctgc gtgtcaacgg cgtcgacctc ttcacccggc
     7321 gcatcgggac cgggccgctc gtcgtggtgc tgcacggcgg cccgggcgcg caccacgatt
     7381 atctcctgcc gcaattcgac cgcctctccg cacggcggga gttgttctac tacgctcagc
     7441 gcggcggcgg ccgttcaccg gtcgcccgtg atgtccccgt cggttggcag cagcacgtcg
     7501 ccgacctgga ggcggtgcgt gagctgctgg ggctcgagcg gttgacgctg tgcggctact
     7561 cctggggagg actgctggcg gtcctgtatc tcct
//
LOCUS       DBNGZW010000434         6115 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1267199, whole genome shotgun sequence.
ACCESSION   DBNGZW010000434 DBNGZW010000000
VERSION     DBNGZW010000434.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6115)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1267199"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            52..2844
                     /locus_tag="AC16JC_10285"
     CDS             52..2844
                     /locus_tag="AC16JC_10285"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR5165122.1"
                     /translation="MSEIALYRRLFRQARHYWLYFSGLPVLGLLAAPIALLAPVPLKV
                     VVDSVLGTRPPPAWLQAILPGSATATAAVTLLVAAAMLVAVTVLGQLRGLGSNILRTW
                     VGERLVLDFRARLIDQVHHVSLSYHDSRGTADSIYRIQQDAPAIQNIMVDGVVPFISA
                     VFTLVSMFVVIARLDGQLALVALAVSPPLFLLSGVYRPRLRHQSRRVKHLESSALAIV
                     QETLGALRVVKAFGREEGEKGRLLQRLNEGVRARIRLALLEGGYGLLIGFVMSLGVAA
                     ILLVGVRHVGAGVLTLGQLLMVLSYAGQLYDPLKTMSTKVAGLQSHLASAERVFGLLD
                     QRPEVTERPHARALERAAGALAFHGVSFRYEPGRPVLQEVSFEIQPGTRLGVVGASGA
                     GKTTLISLLSRFYDPTAGQILLDGIDLRDYKLADLRRQFAVVPQEPVLFSVSIAENIA
                     YARPGAGRDEIVAAAQAANAHEFIARLPRGYDTTVGERGAQLSGGQRQRIAIARAFLK
                     DSPVLILDEPTSAIDPEGEVAIRGAMRHLMRGRTVILITHRPSMLEGCDSLVVLENGR
                     VETEGRVPSTARPQVVAAVSGRCSHVASHPAVQAWGRLHPQREPRAITPLRVRRKKNQ
                     VYRLEGVGEGGGGVIAKRCPKPVAAVERAVYDGILPRLEVASLRHYGSLEESDSEYAW
                     LFIEEASGSEYSNLLSEHRGRAARWLARVHTAAADGTAPPGLPDAGPARYLTLLRSIR
                     DGLPQHLGNPVLSDDDVDFIERLQARLDDLEARWDRVEDVCAGLPQTLVHGDFNGKNI
                     RLRTTRGDATILVFDWEYAGWGVPAVDLAQLAVPSGRLGANPDLPTYHATLRQRWPGV
                     SLEACRRLACCGTLCRTLSALSWDAQHLAFDWAHGYVAGMRLYAAEMDQALERLGWNP
                     TTRLALDPACSAGAPS"
     gene            2841..4007
                     /locus_tag="AC16JC_10290"
     CDS             2841..4007
                     /locus_tag="AC16JC_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165123.1"
                     /translation="MSLTVCLTPADTIGYPQGGGHLWVYLQWALALRAAGCRVIWLEG
                     IDLDDGGSSPARRRRWRNRDVGQCTEILKTRLAAFGLPDSLALYSMSGAPLPRELADG
                     ALDLEAAAEADLLLNLWHSLPPWVVRRFRRTAFVDTDPGTLQVWMTTGDIYLAPHDIY
                     FTIGETVGTPAARFPDGGLRWHYTPPPVHLPEWTPAPAEPGAPYTTITHWWGGTFKFR
                     GMTFSNEKWVSYLEHAELPSRTTAKLELAVCLGAHFEEWRERLEPMGWRLREAWEVSA
                     TPEQYRAYIRGSRGEFSCVKPYCVLFANGWTSDRTICYLASGKPAVVQHTGPSRFLPE
                     AEGLFRFRNLHEAEHALATIERDYERQCRLARALAEEHFDGTRVIQRILAQAMD"
     gene            4034..5080
                     /locus_tag="AC16JC_10295"
     CDS             4034..5080
                     /locus_tag="AC16JC_10295"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR5165124.1"
                     /translation="MAIAGTRLADVLEESQQPGVAELSALLHEMLDGQLGAHQPIHLE
                     RLKREVYRLRLGNGAGSSWVLKRLKPATAQTDRLVAERWLPVIGLGHCCPELLGAAAQ
                     RAGDWVWHVYEDLGEASLAADRQPWRLAAAIDRLAELHARGTLHPLLPEIRWLSRDTG
                     AHFLTANLRDAIATLETLPVAARDVPPQFPASHARLLQRLRMWLDDAPRRVRLLEQEG
                     GPDTLLHGDLWPKNVFVSGTADVPRACLIDWDHVGVGPFSYDLSTLLYRSDPGERAWI
                     QQRYRRAMDRAGWRLPGAEEVNLLCHTAEVARCVHCIVYDAMAVLSDGAAWAFDELMD
                     FERWLETLRSPLPD"
     gene            5089..5778
                     /locus_tag="AC16JC_10300"
     CDS             5089..5778
                     /locus_tag="AC16JC_10300"
                     /inference="COORDINATES: protein motif:HMM:NF016668.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HIR5165125.1"
                     /translation="MKWLIVTADDFGISPGINRGILKAHREGVLTSTSLMVDRPAAEE
                     AAALGGAYRTLSVGLHLELDAVEPERVPEELDRQLARFEELCGFRPTHADSHHDVHRD
                     PRVLPYVLAWARNAGLWVRGHSLVRHLSKFYGWWGGETHPEQITVDGLLRLLDGELRD
                     GVTELSCHPGHVEVGFPSNYAAERELELQTLCDRRTRRGIRDRAIHLIGFRDLPEVMA
                     VAASPEPGGPT"
     gene            5775..>6115
                     /locus_tag="AC16JC_10305"
     CDS             5775..>6115
                     /locus_tag="AC16JC_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165126.1"
                     /translation="MITAVVSVYKVANFPDGGGHFWVYMQYAQGLRRMGCDVYWLEQI
                     NLPGDSDAGRRLLSTFYERMKRFGFEGKTLLYAVDRRRRDAGSFHFIGCSWRDAAAVL
                     RRADLLLNFHYA"
BASE COUNT          881 a         2067 c         2160 g         1007 t
ORIGIN      
        1 cgaccgccat ggttggtgag accgtcggcg gccgccgcgc ggaacactgc gatgagtgag
       61 atcgccctgt accggcggct gtttcggcag gcgcgtcact actggctcta tttctccggc
      121 ctccccgtcc tcgggttgct cgcggctccg atcgccctgc tcgccccggt tccgctcaag
      181 gtcgtcgtgg attccgtgct cgggacgcgg cccccgccgg cctggctcca ggcgattctg
      241 ccggggtccg cgacggcgac cgcggcggtt acgttgctgg tggccgccgc catgctcgtg
      301 gcagtcaccg tgctgggcca gctgcgcggg ctcgggagca acatcctgcg cacctgggtg
      361 ggggagcgtc tggtcctcga cttccgcgcc cgcctgatcg accaggtcca tcacgtctcc
      421 ctgtcatacc acgattcgcg cggcaccgcc gactcgatct accgcatcca gcaggacgct
      481 cccgcgatcc agaacatcat ggtggacggc gtggtgccgt tcatctcggc ggtgttcacg
      541 ctcgtgtcga tgttcgtggt gatcgcccga ctggatgggc agctggcgct cgtggcgctc
      601 gccgtctccc ccccgctctt cctcctcagc ggcgtgtacc gtccccgcct gcggcatcaa
      661 tcgcgccgcg tgaagcatct cgaaagctcg gcgctcgcca tcgtgcagga aaccctcggc
      721 gcgttgcggg tcgtgaaggc gtttgggcgg gaagagggag agaagggtcg actgttgcag
      781 cgtctgaacg aaggcgtgcg cgcgcgtatt cgcctggcgc tcctcgaggg cggctacggg
      841 ctgctcatcg gattcgtgat gtcgctggga gtggccgcca ttctgctcgt cggcgtgcgt
      901 cacgtgggcg cgggcgtcct gacgctcggc caactgttga tggtgctgag ctacgcgggc
      961 caactctacg atccgctgaa gaccatgagc acgaaggtgg cgggactgca gtcccacctc
     1021 gcgagcgccg agcgggtctt cggactgctg gaccagcgcc ccgaggtgac cgaacgaccg
     1081 cacgcccgcg cgctggaacg cgccgccggc gcgctggcgt ttcacggcgt gagttttcgc
     1141 tacgagcccg gccgcccggt cctgcaggag gtgtccttcg agatccaacc ggggacacgg
     1201 ctgggcgtcg tcggggcctc gggagcgggc aagaccaccc tcatcagtct actgagccgc
     1261 ttttacgacc cgacggcggg acagatcttg ttggacggga tcgacctgcg ggactacaag
     1321 ctcgccgatc tccgccgcca gttcgccgtc gtcccgcagg agccggtgct gttttcggtc
     1381 agcatcgcgg agaacatcgc ctacgccaga ccgggggccg gccgggacga gatcgtcgca
     1441 gccgcacagg cggccaacgc ccacgagttc atcgcgcggc tgccccgggg ctatgacacc
     1501 accgtgggcg agcggggagc ccagctgtcg ggcggccagc gccagcgcat cgccatcgcg
     1561 cgcgccttcc tcaaggacag ccccgtgctc atcctggacg agcccaccag cgcgattgat
     1621 cccgaggggg aagtggcgat ccgcggcgcg atgcgccatc tcatgcgtgg ccgcaccgtg
     1681 atcctcatta cccatcgccc gagcatgctg gaaggctgcg actccctggt cgtgctcgag
     1741 aacgggcgcg tcgagaccga agggcgcgtg ccctcgaccg cgcggccgca ggtcgtcgcg
     1801 gcggtgagcg gccggtgctc gcatgtcgcg agccatccgg cggtccaggc ctggggccgg
     1861 ctgcacccgc agcgggagcc gcgggcgatc acaccgttac gcgtgcgcag aaagaagaac
     1921 caggtgtacc ggctggaagg cgtgggggag ggcggcggtg gagtgatcgc caagcggtgc
     1981 ccgaagcccg tagcggcggt cgagcgggcc gtgtacgacg ggattctccc ccggctcgag
     2041 gtcgcgtcgt tgcgccatta cggctcgctc gaggaatcgg acagcgaata cgcctggctg
     2101 ttcatcgaag aagccagcgg cagcgaatac tcgaatctcc tctccgagca ccgcgggcgt
     2161 gccgcccgct ggctcgcgcg ggtccatacc gctgccgccg acggtaccgc tccgccgggt
     2221 ctgccggatg ccggcccggc ccgctacctg acgctgttgc gctcgattcg cgacgggctg
     2281 ccgcaacacc tgggcaaccc ggtcctgtcc gatgacgacg tcgacttcat agaacggctg
     2341 caggcgcgcc tcgacgatct ggaagcgcgg tgggaccggg tggaggacgt ctgcgcgggc
     2401 ctgccgcaga cgctggtgca cggcgacttc aacggaaaga acatccgcct gcgcaccaca
     2461 cgcggcgacg cgacgatcct cgtgttcgac tgggagtacg cagggtgggg cgttcccgcc
     2521 gtcgacctcg cccaattggc ggtcccatcc gggcgcctgg gggccaatcc cgacctgccg
     2581 acctaccacg cgacgctgcg gcagcgctgg ccgggagtca gtctggaggc gtgtcggcgg
     2641 ctcgcctgtt gtgggacgct atgccgcacg ctgtccgcgc tctcctggga cgcgcaacac
     2701 ctggccttcg attgggccca tggctacgtc gccggcatgc ggctctacgc cgccgagatg
     2761 gaccaggcgc tcgagcgcct gggatggaat cccaccacgc gcctggccct cgatccggcg
     2821 tgcagcgcgg gggcaccgtc gtgagcctca ccgtatgcct cacgccggcg gacaccatcg
     2881 gctatcccca gggcggggga catctctggg tctatctgca gtgggcgctg gcgctgcggg
     2941 cggcgggctg ccgggtgatc tggctggagg gcatcgatct cgacgacggc ggcagttcgc
     3001 ccgcgcgtcg gcgccggtgg cgcaaccgtg atgtgggcca gtgcacggag atcttgaaga
     3061 cccgactggc ggcattcggc ctcccggact cgctggcgct gtattcgatg agcggcgcgc
     3121 cgttgccgcg ggagctggcg gatggcgctc tcgatctgga ggcggccgcg gaggcggacc
     3181 tgctcctcaa cctgtggcat tcgctgccgc cgtgggtcgt gcggcggttc cgccgcaccg
     3241 cgttcgtgga caccgacccc gggacgctcc aggtgtggat gaccacgggc gacatctacc
     3301 tggcgccgca cgacatctac ttcaccatcg gtgaaacggt ggggacgccg gccgcgcgat
     3361 ttcccgacgg tggcctgcgc tggcactaca ctccgccgcc ggtccatttg ccggagtgga
     3421 cgccggcgcc agccgagcct ggcgcgccct acacgacgat cacgcactgg tgggggggga
     3481 cattcaagtt ccggggcatg acgttctcca acgagaagtg ggtgtcctac ctcgagcacg
     3541 ccgagcttcc ctcccgcacc accgcgaagc tggagctggc cgtatgcctg ggtgcgcact
     3601 tcgaggagtg gcgtgaacgg ctcgagccca tgggctggcg ccttcgcgag gcgtgggaag
     3661 tgagtgcgac gcccgagcag taccgggcgt acatccgggg gtcgagaggg gagttcagct
     3721 gcgtcaagcc gtattgcgtc ctgttcgcga acggctggac cagtgaccgg acgatctgtt
     3781 acctcgccag cggcaagccc gcggtcgtgc agcacaccgg ccccagccgg ttcctgcccg
     3841 aggccgaggg gctgtttcgc ttccgcaacc tgcacgaggc cgagcacgcg ctggccacga
     3901 tcgagcgcga ttatgagcgg cagtgccggc tggcccgggc cctggcggaa gagcacttcg
     3961 acggcacccg ggtgattcag cggatcctgg cacaggcgat ggactgagaa tgacggaacg
     4021 gtacggcgct gcgatggcga tcgcaggaac acgtctcgcg gacgtcctcg aggagtcgca
     4081 acagcccggc gtggcggagt tgtccgcact gctgcacgag atgctggacg gacagctcgg
     4141 ggcgcatcag ccgatccacc tcgagcgatt gaagcgggag gtgtaccgtc tgcggctggg
     4201 gaacggcgcg ggcagctcgt gggtcctcaa gcgcctcaag ccggccaccg cgcagaccga
     4261 ccgcctcgtg gcggagcgct ggctgccggt gatcggcctc gggcattgct gcccggagct
     4321 gctgggagcg gcggcgcagc gggcgggaga ctgggtgtgg cacgtctacg aagacctcgg
     4381 cgaggcgtcc ctggccgccg atcgccagcc gtggcgcctg gctgccgcga tcgaccggct
     4441 ggccgagctc cacgcgcgag ggacgctgca cccgctgctg cccgagatcc gctggctttc
     4501 ccgggacacc ggggcgcatt tcctcaccgc gaacctccgc gacgcgatcg cgacgctcga
     4561 gaccctgccg gtcgcggctc gggacgtgcc gccgcagttc ccggcctcgc atgcccgcct
     4621 gctccagcga ctccgcatgt ggctcgacga cgctccacgc cgcgtccgcc tcctcgaaca
     4681 ggagggcgga cccgatacgc tcctccacgg ggatctgtgg ccgaagaacg tgttcgtctc
     4741 cggcaccgcg gatgtcccgc gggcctgcct catcgactgg gaccatgtcg gcgtgggccc
     4801 gttcagctac gacctttcca ccctgctcta tcggtccgat cccggggaac gggcgtggat
     4861 ccagcagcgc tatcgccggg ccatggatcg tgccggctgg cgcctgccgg gagcagagga
     4921 agtgaacttg ctctgccaca ccgcggaggt cgcgcggtgt gtgcactgta tcgtgtacga
     4981 cgccatggcc gtactgagcg acggggccgc gtgggcgttc gacgagttga tggatttcga
     5041 gcggtggctc gagacccttc ggtccccctt gccggattga agtggcggat gaagtggctc
     5101 atcgtcacgg ccgatgattt cgggatcagc cccggcatca accgcgggat cctcaaggcc
     5161 caccgggagg gcgtcctcac cagcaccagc ctgatggtgg accggccggc ggccgaggag
     5221 gccgccgccc tggggggcgc ctaccggacg ctgagcgttg ggctccatct ggagctggac
     5281 gcggtcgagc ccgagcgcgt ccccgaggag ctggatcgcc agctcgcccg gttcgaggag
     5341 ctctgcggct ttcgtcccac ccacgccgat tcccaccacg acgtccatcg cgacccacgg
     5401 gtgttgccgt acgtgttggc atgggcgagg aatgccggct tatgggtacg cggtcattcc
     5461 ctggtccgcc acctgtcgaa gttctacggt tggtggggcg gcgaaaccca tcccgagcag
     5521 atcaccgtcg acggcctgct gcgcctgttg gacggtgagc tgcgggacgg cgtgaccgag
     5581 ctgagctgtc acccgggcca tgtcgaggtg gggtttccgt cgaactacgc tgcggagcgc
     5641 gagctggagt tgcagacgct gtgcgacagg cggacgcgtc gggggatccg tgaccgggcg
     5701 atccatctga tcggttttcg ggatctcccc gaggtcatgg ccgtcgccgc atctcccgaa
     5761 ccgggagggc cgacgtgatc acggccgtcg tgtcggtcta caaggtcgcc aatttccccg
     5821 atgggggagg tcatttctgg gtgtacatgc agtacgccca gggcctgcgg cgcatgggat
     5881 gcgacgtcta ctggctggag caaatcaacc tccccggcga ttcggacgcc ggccgacgcc
     5941 tgctctcgac cttctacgag cgcatgaagc ggttcgggtt cgagggcaag acactgctgt
     6001 atgcggtgga ccgccggcgg agggacgccg gctccttcca ctttatcggg tgttcctggc
     6061 gggacgccgc agccgtgctg cgacgggcgg atctcctgct caactttcac tacgc
//
LOCUS       DBNGZW010000435         3260 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_793093, whole genome shotgun sequence.
ACCESSION   DBNGZW010000435 DBNGZW010000000
VERSION     DBNGZW010000435.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3260)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3260)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3260
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_793093"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(209..2563)
                     /locus_tag="AC16JC_10310"
     CDS             complement(209..2563)
                     /locus_tag="AC16JC_10310"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HIR5165127.1"
                     /translation="MSSCPIRRFVAVPLLACLVGSRAAGQTPAPAEHYETVFAQLQAM
                     TLQADRAAAVRNVTLRRDAIVLHLDEGQLFPTTAVAGRVVAAVFVGRGSVSFAPPLPI
                     ERAELNRVLGDTVIDAPITAAVLVFTDSTEAELGRQSTTVTGANSSPAADPLHDALDL
                     LLDRDTRFVTQGTWMSAILNGETDGFFYVHVKRAHGRDLMLVVDPDQPEAVALLRRGG
                     ANGPKVQVVAQFRRAERLSDTTNVVNGALDPLQMQSYRIESTIAKGLGFSAVATARFT
                     ARHDGIRWAPFRLYSELQVDSVAERAGATDTFFRMKHSPLLWVRLDPAVRAGETRELR
                     VAYHGNLIGFGSLMSQFMPSGAARFNLPPSMDSWLFVKASQTWFPRYGNDQAATFDLV
                     FHVPKRYHLASVGRLESSRVDGDVATTHWVMDQPTDQACFNLGEFEEVKITDPRIPPV
                     TVQMNSEAHRHLEEIFRGQAHPQQDVASDVANSLAFFSQVYGAPLYSQYYATEVPFSY
                     GQAFPGLMYLSVWTFQSLDESGGEETFRAHEMAHQWWGIGVTPAGDRDAWLSEGFADF
                     SGLWYMQIILHDNDKFFKELDARRRRIRSHGSDVPPIGIGWRASQTNDPSDYSLVIYE
                     KGAWVLQMLRNMMLNLRTMNEDAFTAMMKDFYTKYRGGRASTADFQRTAEQHLGLSLG
                     WFFDEWVDGTAIPTYTLSWTTERQADSSYTLHLRVRQEGVGPQFLMPVPLRIQFADST
                     HAFVRVNVRGPVTEGTVRVPAAPVQLELNPLMSVLAEVKTEPWR"
     gene            complement(2626..>3260)
                     /locus_tag="AC16JC_10315"
     CDS             complement(2626..>3260)
                     /locus_tag="AC16JC_10315"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR5165128.1"
                     /translation="RLNDLGKVLRERQDRFEDMLGKLLSAAQLKSYRQWRDQQDRGIA
                     EQQREDALRWRIMPEGFAGAGGFEPRRDEQKTTLTVPGVESPAIGAQVVRVGRTLYIA
                     GQLPLDSAGNLVGGDNLAQQAKQAFANLSTVLRGAHANARDVVELTIYVAGYRPADLA
                     TIRDAAAELFPSWSAPVVTVLGVQALAQEGARIAVAATALAGPPGGSADR"
BASE COUNT          532 a         1089 c         1116 g          523 t
ORIGIN      
        1 gtccggtcct ttggtcgagc cacgggctgc cccccagcag cggcgcccga tggcgacgca
       61 aaggttccag cgaaagggat ccgggagtct gccccccttt gcctcacgag gaccctcgct
      121 gggggagggt cgatgaggtt gggcgtggat cgccgcccct aggaaatatt ttgcacaaac
      181 cgtcgccgct cggcaagacc ccggcgattc accgccaggg ttcggtcttc acctcggcga
      241 gcacggacat caacgggttg agctcgagtt gtaccggcgc cgcgggaacc cgcaccgtgc
      301 cctcggtcac cggcccgcgc acgttcacgc gcacgaatgc gtgagtggaa tccgcgaact
      361 ggatgcgcag cgggaccggc atgaggaatt gcgggccgac cccttcctgc cgcacgcgca
      421 ggtgcagggt gtacgagctg tcggcctgcc gctccgtcgt ccacgacagg gtgtaggtcg
      481 gaatcgccgt gccgtcgacc cactcatcga agaaccagcc gagggagagc cccagatgct
      541 gctcggccgt gcgctggaag tccgccgtgg acgcccggcc gccgcgatac ttggtgtaga
      601 aatccttcat catcgcggtg aaggcgtcct cgttcatggt ccggaggttg agcatcatgt
      661 tccggagcat ctgcaacacc cacgcgcctt tctcgtagat cacgagcgaa tagtcgctcg
      721 ggtcgttcgt ctgtgacgcg cgccagccga tgccaatcgg aggcacgtcg ctgccgtgcg
      781 agcggatgcg gcggcgacgc gcgtccagtt ccttgaagaa cttgtcgttg tcgtgcagga
      841 tgatctgcat gtaccacagt ccggagaagt cggcgaaccc ctccgacagc caggcgtcgc
      901 ggtctccggc gggcgtcacc ccgatgcccc accactggtg cgccatctcg tgcgcccgaa
      961 acgtctcctc gcccccgctt tcgtccagcg actggaaggt ccacacggac agatacatca
     1021 acccggggaa cgcctgaccg taggagaagg gaacttccgt ggcgtagtac tgactgtaga
     1081 ggggtgcgcc gtagacctgg gagaagaacg cgaggctgtt cgcgacgtcc gacgcgacgt
     1141 cctgctgcgg gtgggcctgt cccctgaaga tctcctcgag gtgccggtgc gcctcgctgt
     1201 tcatctggac cgtgaccgga gggatgcggg ggtcggtgat cttgacctcc tcgaactcac
     1261 cgaggttgaa gcaggcctga tcggtcggtt gatccatcac ccagtgggtc gtggccacgt
     1321 caccgtccac ccgggagctc tcgagacgac cgacactggc gaggtgatac cgtttgggta
     1381 cgtggaagac cagatcgaag gtcgcggcct ggtcgttccc gtagcggggg aaccaggtct
     1441 gcgacgcctt gacgaacagc cagctgtcca tgctcggcgg cagattgaat cgagccgcgc
     1501 cggacggcat gaactgcgac atgagcgacc cgaagccgat gagattgcca tggtaggcca
     1561 cgcgaagctc acgggtttcg ccggcccgga ccgccggatc gagccggacc cagagcaggg
     1621 gactgtgctt catccggaag aacgtgtcgg tcgctccggc gcgttcggcg accgaatcga
     1681 cctgcaactc gctgtacagc cggaagggcg cccaccgtat gccgtcgtgc cgggcggtga
     1741 agcgcgccgt cgcgaccgcc gagaacccca ggcccttggc gatcgtggac tcgatgcggt
     1801 aggactgcat ttgcagcggg tcgagcgctc cgttcaccac gttcgtcgtg tcgctcaacc
     1861 gctcggcacg ccggaattgc gcgaccacct gaaccttcgg accgttcgcc cccccgcggc
     1921 gaagcagggc gacggcctcc ggctggtccg gatccacgac cagcatcagg tctcggccgt
     1981 gggcgcgctt cacgtggacg taaaagaacc cgtccgtctc gccgttcagg atggccgaca
     2041 tccaggtccc ctgcgtgacg aaacgcgtgt cgcggtcgag caggaggtcc agggcgtcgt
     2101 gcagcggatc cgcggcggga ctggagttcg caccggtcac cgtggtggac tggcgtccga
     2161 gctctgcctc ggtggaatcg gtgaacacca gcaccgcggc ggtgatcggc gcgtcgatca
     2221 ccgtatcgcc gagcacgcgg ttcagctcgg cacgctcgat cggcagcggc ggtgcgaacg
     2281 acaccgatcc acgcccgacg aacacggcgg caaccacgcg gccggccact gccgtcgtgg
     2341 ggaacagctg accctcgtcg aggtggagca cgatcgcatc gcgccgcagc gtcacgttcc
     2401 gcacggcagc cgcgcggtcc gcctgcaggg tcatcgcctg cagctgtgcg aagacggtct
     2461 cgtaatgctc ggcgggggca ggcgtctggc ccgccgccct cgacccgacg aggcacgcaa
     2521 gcaggggaac ggcgacgaag cggcggatcg gacaggacga catgggcgct cccatcggga
     2581 gacgccgtta tgataggggc tcttgcgccg cctggcgaga cggggctacc ggtctgcgga
     2641 gcctcccggc ggcccggcga gcgcggtcgc cgcgaccgcg atgcgtgcgc cttcctgggc
     2701 cagcgcctga acaccgagga ccgtcaccac cggcgcgctc caggacggga acaactccgc
     2761 cgccgcatcg cggatcgttg cgaggtcggc agggcggtag ccggccacgt agatcgtgag
     2821 ctcgacgacg tctcgggcgt tggcgtgggc gccgcgcagc acggtggaga gattggcgaa
     2881 cgcctgcttc gcctgctggg ccaggttgtc acctccgacc aggttgcccg cgctgtcgag
     2941 cgggagttgg cccgcgatgt acagcgtgcg gccgacgcgg accacctggg cgccgatggc
     3001 cggagactcc actcccggga cggtcagcgt ggtcttctgc tcatcccgcc gcggctcgaa
     3061 gccaccggcg ccggcaaaac cctcgggcat gatccgccag cgcagcgcgt cctcgcgctg
     3121 ttgctcggcg atgccgcggt cctgctggtc gcgccactgg cggtagctct tgagctgggc
     3181 ggcggagagc aatttgccca gcatgtcttc gaacctgtcc tggcgctccc gcaacacttt
     3241 gcccaggtcg ttgaggcgct
//
LOCUS       DBNGZW010000436         2683 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_382533, whole genome shotgun sequence.
ACCESSION   DBNGZW010000436 DBNGZW010000000
VERSION     DBNGZW010000436.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2683)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2683)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2683
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_382533"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2036
                     /locus_tag="AC16JC_10320"
     CDS             <1..2036
                     /locus_tag="AC16JC_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165129.1"
                     /translation="TAEEAITDYHARVQERISLGLRALRRDRTFYQREMAARIAWHRY
                     GPDTVTMLGAREAIPVVYANTRLPEDLDTDAPDLAFEPGRGRIDIGIGDSEFVYDPLA
                     PDGESQYRFQSGDTTVITLQDGRTIRLLELRIIPRRDDFHLLTGSFWIEDEHAALVRA
                     VFRPARAWDLERDLTSDPEDSSDAKGIPGFLKPIRGEVRYITAEYALVDGKWWLPRLI
                     AFDAVATAGSFLTAPLRYERVFSDFDVVASTAGGLAATDASQADSLERDTVPPDSAAI
                     IACQGRAGCRCTRRVCRTAVVIVPPDTASLLTSPYLPRSFDDDGAFLSSSEARELGRA
                     VGLLPQTPWHAHAPSLHFGPGGTGLLRYNRVEALSVGARLGLDFGRLTADATARIGTA
                     DQWPNLELGVEHAATDLSLRVAGYRRLAAANPDTRPLGIGNSLGALVLGRDDGVYYYG
                     WGGELLLRPAPTAARSYEWRLFAEWQRPADKETDLSLPHLLRDRHEFRPNVRADIARE
                     FGTTITLRGARGVSTRGLTVGAELTMDANLGTYEFARTSLTTRLTAPLPRRLLAAVEL
                     AAGATGGDAPVQSLWYLGGPATLRGYPGGVIAGPDFWRARVEVANASPGVRLAAFGDA
                     GWAGLGTAFTRGRPLVAAGIGASFLDGIVRVDLARALVSPKGWRMDFYVDGIL"
     gene            2140..>2683
                     /locus_tag="AC16JC_10325"
     CDS             2140..>2683
                     /locus_tag="AC16JC_10325"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR5165130.1"
                     /translation="MPQSLPDIDPADLASVPLERAETIPSAWYTDPRFHALEREAVFG
                     RSWQYIADRTALAAPGHVVLADVAGEPIIAVRDRADALHAFFNVCRHRGGPLALKDED
                     CDMLICKYHGWTYRLDGMLRGVPHFNRVELFDREDYGLTRVPLALWEGLAFVHLGPSP
                     EPIEATFAGIRERVAPTRLGA"
BASE COUNT          359 a          941 c          955 g          428 t
ORIGIN      
        1 ccacggccga ggaggcgatc accgactatc acgcccgcgt gcaggagcgg atttcgctcg
       61 ggctgcgggc gctgcgccgc gaccggacgt tctaccagcg tgagatggcg gcgcggatcg
      121 cctggcaccg gtacggcccc gatacggtca cgatgctggg cgcgcgggaa gcgatccccg
      181 tcgtgtatgc gaacacgcgc ctccccgagg atctggacac ggatgccccg gatctggcct
      241 tcgagccggg tcgcggccgg atcgacatcg ggatcggcga ctcggagttc gtctacgacc
      301 cgctcgcgcc ggacggagaa tcgcagtacc gcttccagtc cggcgatacc acggtcatca
      361 ccctgcaaga cggacgcact atccgcctgc tcgagctgcg catcatcccg cggcgcgacg
      421 attttcacct gctcaccggc tcgttctgga tcgaagacga gcacgccgcg ctggtgcgcg
      481 ccgtgttccg gccggcccgg gcctgggatc tggagcggga cctgacgagc gacccggagg
      541 actcgagcga cgcaaagggc attcccggtt ttctgaagcc gatccgtggc gaggtccgct
      601 acatcaccgc cgagtacgcg ctggtcgacg ggaaatggtg gctgccgcgg ctgattgcgt
      661 tcgacgccgt tgcgacggcc ggctcgtttc tcactgcgcc gttgcggtac gaacgcgtgt
      721 tctccgattt cgacgtggtc gcctcgacgg ccggcggtct cgcggcgacc gatgcgtccc
      781 aggcggattc gctcgagcgc gatacggtcc cgcccgactc ggcggcgatc atcgcctgtc
      841 agggacgcgc cggctgccgc tgcacgcggc gcgtctgtcg caccgccgtg gtcatcgtcc
      901 ccccggacac ggccagcctg ctcacgagcc cgtacctgcc acgctccttc gacgacgacg
      961 gcgctttcct gtcttccagt gaggcccgcg agctgggacg ggcggtggga ttgttgccgc
     1021 agacgccctg gcacgcgcac gcgccgtcgc tgcacttcgg tccgggaggc accggcctgc
     1081 tgcggtacaa ccgggtcgag gcgttgtcgg tcggtgcgcg tctggggctg gatttcggcc
     1141 gcctgacggc ggacgcgacc gcgcggatcg gcaccgcgga ccaatggccc aatctcgagc
     1201 tcggcgtgga gcacgcggcg accgacctgt cgctgcgtgt cgcgggatac cggcgtctcg
     1261 ccgcggcgaa ccccgacacg cggccgctcg gcatcggcaa ctccctcggc gcgctcgtgc
     1321 tggggcggga cgacggggtc tactactacg ggtggggtgg ggagctgctg ctgcggccgg
     1381 cccccaccgc cgcgcgcagc tacgaatggc ggctgttcgc ggaatggcag cgcccggcgg
     1441 acaaggagac cgatctgagc ctgccgcacc tgctgcgcga ccggcacgag ttccggccca
     1501 acgtacgcgc cgacatcgcc cgggaattcg ggaccaccat cacgctccgg ggggcgcgcg
     1561 gcgtgtccac ccggggactc acggtcggtg cggagctcac catggacgcg aaccttggca
     1621 cctacgagtt cgcgcgaacc agcctgacga cccggctgac ggcgccgctc ccgcgacgcc
     1681 tgctcgcggc cgtagagctg gccgccggcg ccacgggggg cgatgcgccg gtgcagagcc
     1741 tgtggtacct cggtggaccg gcgacgctgc gcggctatcc gggcggggtg atcgccgggc
     1801 cggacttctg gcgcgcgcgg gttgaggtgg cgaacgcctc acccggtgtc cgtctcgcgg
     1861 ccttcggcga cgcagggtgg gcgggcctcg gcacggcgtt cacccgcggg cggccgctcg
     1921 tcgccgcggg catcggggcg tcgttcctgg atggcatcgt gcgcgtggac ctcgcccggg
     1981 cgctcgtcag cccgaagggg tggcggatgg atttctatgt ggacgggatc ctgtaactga
     2041 cgctatcggc tatccgctat cggctatcgg ccggtggcat cgaattgccg atagctgata
     2101 gccgatagcc gatagccgat acccgtgtag tttcgccccg tgccgcagtc cctccccgac
     2161 atcgatcccg ccgatctcgc ctccgtgccg ctcgagcggg cggagacgat cccgtccgcc
     2221 tggtacacgg acccccgctt tcacgcgctc gagcgcgagg cggtgttcgg ccggagctgg
     2281 cagtacatcg cagatcggac ggcgctcgcg gcaccgggcc atgtggtgct cgccgacgtc
     2341 gccggtgagc cgatcatcgc cgtccgcgac cgggcggacg cgctccacgc cttcttcaac
     2401 gtgtgtcgcc atcgtggtgg cccgctcgcg ctgaaggacg aagactgcga catgttgatc
     2461 tgcaagtatc acggctggac ctaccgtctc gacggcatgc tccgtggcgt gccgcacttc
     2521 aaccgcgtgg agctgttcga cagggaagac tacgggctca cgcgcgtgcc cctcgccctg
     2581 tgggagggcc tggcgttcgt ccatctgggt ccgtcgccgg agccgatcga ggcgacgttc
     2641 gccgggatcc gcgagcgcgt ggcgcccacg cggctcgggg cgc
//
LOCUS       DBNGZW010000437         9656 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_793270, whole genome shotgun sequence.
ACCESSION   DBNGZW010000437 DBNGZW010000000
VERSION     DBNGZW010000437.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 9656)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9656)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9656
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_793270"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..416
                     /locus_tag="AC16JC_10330"
     CDS             <1..416
                     /locus_tag="AC16JC_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR5165131.1"
                     /translation="PRMYFAMARDGVFFRALGDVHPRFGTPAVAIAVGGAWAALLTLW
                     GSFEQLLTYVVFASWLFAALAAASVFVLRRRRPDAPRPFRVPGYPLTPAVFILAAVAI
                     VANTVVARPVQAFAGIGIVLLGTPAYLFWRRRAVS"
     gene            complement(438..2051)
                     /locus_tag="AC16JC_10335"
     CDS             complement(438..2051)
                     /locus_tag="AC16JC_10335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165132.1"
                     /translation="MQRILGIVIVLANGPTGQGDNGIAATPPRSSCDAVLEAQLPNPD
                     TLVVRIREIQCHSVKLVIVGPTQRMTSTSAWWFSTAYQVPVVVENASNVPITLPIAMT
                     VDSAVAVQRGRQLNAIYSRRYVDFPFWEGREPQQPWRFVAGRNGGTRLLPGAQTGAQH
                     LTIASWPLTQGFRVWFRIEGTFPEPRQQAAIPAPARPKADTTPAGPVIRQFVEAFRFP
                     LQRAIRTLYRDTTRDLVIFDVVYGHAQDCLAGCFYSNAIGMAYGRKLGWLHVYDYENE
                     GLAGTSSRVNQALLRIDESDSYLLSGQLLDALDPIFGGESPLVDQVVLPMTVESPHVY
                     RALVFRLIGDLYAHVNQPRASLLVRTPTVQGDPELLTLMAGLPDPTYETVRDAARRTL
                     HGMGRAVAADRDASTRALFVLAQTLQLPVDSGIRRALLHHRRVRDNPAILAILGDQPQ
                     LLAAVQASERVKSMLADYLMPRRRWVQADSSYGWALLADSEAGQNQDVLLVLANLPIS
                     LDQGIVWAASRRLPEGSLRRWEPDYIPMP"
     gene            2237..3439
                     /locus_tag="AC16JC_10340"
     CDS             2237..3439
                     /locus_tag="AC16JC_10340"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR5165133.1"
                     /translation="MKTQGSQTDIVFLSGVRTGFGAFGGSLKEFSAIDLAAIAARNAL
                     ERSGVPAADIGHAIFGNALQTSADAIYMARHVALKAGLPIETPAVTVNRLCGSGFEAI
                     TQGAQLIILGETEAVLAGGGESMSQAPHVVRGARWGLRLGPAPLEDLLWEALKDPQCG
                     FSMAETAENLADKYKLGRTEVDQVAVDSQQRAKKAWDDCVFTDEVIPVPVKKKGQTVE
                     FRADEHMRPETTLEGLAALKPYFKKDGVVTAGNASGICDGAAATVIASEAYAKAHGLK
                     PLGRLVAWAVVGVEPKYMGIGPAPSARKVLQKAGMTLAEMDLVEINEAFAPQYLAVEK
                     ELGLDRAKTNIHGGAIAIGHPLAASGTRITLHLLHALRRTGRRFGLGSACIGGGQGLA
                     VIVEAFPA"
     gene            3449..4300
                     /locus_tag="AC16JC_10345"
     CDS             3449..4300
                     /locus_tag="AC16JC_10345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005992715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HIR5165134.1"
                     /translation="MGDFFLGIMAGLGLYLFSGLRVLREYERAVYFFLGRSWGAKGPG
                     LIYLPPLFARMQKVSLRVMALDIPPQDVITRDNISIKVNAVLYMLVRDPVKAVIGVEN
                     YLYATSQLAQTTLRSVLGETELDELLANREKINAILKKIIDQRAEDWGIEVSAVEVKD
                     VDLPPEMKRAMARQAEAERERRAKVINAEGELQASEKLAQAAAVIGREPAAIQLRYLQ
                     TVTEIASENNSTTIFPIPIDIFRGILDAVSRPRPDETATPAALPARTAGESLSLPSVP
                     GKDKVKA"
     gene            4303..5124
                     /locus_tag="AC16JC_10350"
     CDS             4303..5124
                     /locus_tag="AC16JC_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165135.1"
                     /translation="MAGELISREALDRIIRRAAELQTGERDIGEGLTEAEVEALGNDV
                     GIPARYLKQALLEERTRLPDEDAGRGLTWSLVGPGRLNAHRVVPGDRAAVERALSAWM
                     ESEELLRVKRRYTERTTWEQQVGFMASMQRGLRGGGRRYALAQAEEVAGQVTQLEPGF
                     CHVRLSADVRNLRRARIGGAVTLAGLGAAGSVVAGTIGVLAPFLALPAVGFAGLALLA
                     LVAGRRTQLGRIQVALEQVLDRLERGEIRSEHVLPGPRASAFVRIAEEIRKQFQI"
     gene            5135..6022
                     /locus_tag="AC16JC_10355"
     CDS             5135..6022
                     /locus_tag="AC16JC_10355"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5165136.1"
                     /translation="MSDITRVGVLGCGLMGSGIAQVAATAGYETIVRDVAQPILDKAK
                     AGIEKSLAKFVEKGKLQAPDRDAALRRLSFTTVTADLKACDVVVEAVTEDLEVKNALW
                     RELDALCPAGTIFASNTSSLTIAAMAAATKRADRFVGLHFFNPVPLMQLVEVVRTVTT
                     SEDAFKRAFAFARSLGKEPVAAKDNSGFIVNLLLVPYLLDAIRALEHGVGSVTDIDKA
                     MQLGCGYPMGPLTLLDFVGLDTTYKIAEIMFAEYKEQRYAPPPLLKRMVLAGMYGKKS
                     GKGFYDYSANPPVVSSLGL"
     gene            6049..6639
                     /locus_tag="AC16JC_10360"
     CDS             6049..6639
                     /locus_tag="AC16JC_10360"
                     /inference="COORDINATES: protein motif:HMM:NF021505.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HIR5165137.1"
                     /translation="MFGYDLPRLHAAVNDLPAALLLAAVLFDLMGWILKRDSLVWAGI
                     WTLWAGVIGGWAAVILGELAEDAVEHGDAVHAIMATHEKLALITMSLFTVVLAWKLFR
                     RFQLRPLEARLLTLLAAVGFVFLLWTGAEGGEMVFDHAAGISTAKLRAEIENREAGHH
                     HGPGEADDHDHDAADSAHDSAAGTHVDPPGTPPHTH"
     gene            6641..7201
                     /locus_tag="AC16JC_10365"
     CDS             6641..7201
                     /locus_tag="AC16JC_10365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165138.1"
                     /translation="MRLRIADCGFWIVRRGAGARRATWADRVKSAIRNPQSAIVLVLA
                     ACSPVTTRPPFLPYPQALVTVLDAPPGRVVPEALGWLTSQGLHVQWSSPQDGYVESAW
                     FNLRTHTSVFGDADPGDLLFTVKIRCWADPNIPGKTQLTVEAVYRPVLDPSRPERDLE
                     EIVPQGSEGYRIAQQLIDALKQKFGT"
     gene            complement(7248..8072)
                     /locus_tag="AC16JC_10370"
     CDS             complement(7248..8072)
                     /locus_tag="AC16JC_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009510556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HIR5165139.1"
                     /translation="MKKHYLLASDFDQTLSFNDSGIILSELLGTSGFPEKVAALARLN
                     LVQQGAELAYLLRHDPEYRRVRQADLVEVGRRVRLKRNVRLLTTLLARGIDGHRFDFY
                     VISAAPEEVVLAALDGIVPPDHIVGTQFGWDTGSGEIESVARVAAGFGKVAAVEERRL
                     QLALPRDQVVYVGDGQSDIPVMLHVNRGDGFTIAASEAREIAQIAKRTVISEDVLSVL
                     VPILEEILAWGPSQIRALFEGHGLVIQEWDRVRTDWLTFRREAESGVVGLDAAAEG"
     gene            complement(8152..8298)
                     /locus_tag="AC16JC_10375"
     CDS             complement(8152..8298)
                     /locus_tag="AC16JC_10375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165140.1"
                     /translation="MNTIAPFFLLVFALSLAARRGYNSAMTRCLMNLLHFTGPRSPGS
                     ARTA"
     gene            8319..>9656
                     /locus_tag="AC16JC_10380"
     CDS             8319..>9656
                     /locus_tag="AC16JC_10380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5165141.1"
                     /translation="MTSTQTDPRIRSAYAEGAGIYRILPEAVAIPRTVDDVVHLVRDA
                     ERGVTPLIPRGAGSGMPGGNVGRGTIVDCSQGFRVLELDPARRIARAGAGVTWAEVND
                     AARPHGLRLPPDPSSGAFATSGGMVATNAAGPRSVRYGSVRPWIEALELVGNGGEIRT
                     IRRGGGAGPWPLTPDTRHLVGQRFPHTRKNSSGYALDAFAASGDELDLYIGAEGTLGI
                     VTTVEWRLDPIPPESAGAALTLGSLDDLGEAVAYLATLDPAAVELLDKTLLEFVAAAG
                     GEVHPGAECLLLVEFERQTAAAARGVVGDAVRGLKHLSMQADTALDRAGLERIWGIRR
                     LASPALARLPETRRSLQVVEDGCVPTARLSGYLAGLRAAAARHEIPVAIFGHAGDGHV
                     HVNALPDLTRPGWRDALQALYEEVQDLLVNLGGTPSGEHGDGRLRGGTLERFFG"
BASE COUNT         1569 a         3288 c         3292 g         1507 t
ORIGIN      
        1 ggccgcgcat gtacttcgcg atggcacgcg acggcgtgtt cttccgcgct ctgggagacg
       61 tgcatccccg cttcggcacg ccggcggtcg cgatcgccgt gggcggggcg tgggcggcgt
      121 tgctcacgct gtggggcagc ttcgagcagt tgctgaccta cgtggtgttc gcgagctggc
      181 tgttcgccgc cctcgcggcc gcgagcgtgt tcgtgctgcg acgccggcgt cccgacgcgc
      241 cgcgcccctt ccgggtgccc ggctacccgc tgacccccgc cgtcttcatc ctcgccgccg
      301 tggcgatcgt cgccaacacc gtggtggcgc ggcccgttca ggcgttcgcc ggaatcggca
      361 tcgtgctgct tggaacccct gcgtatctgt tctggaggcg gcgggcagtg agctgaagcc
      421 gcggatcctg ctgccactta gggcatgggg atgtaatccg gctcccatcg acgcagggat
      481 ccctcgggta gccgacgcga cgcggcccac acgatcccct gatcgaggct gatcggcaga
      541 ttggcgagca ccagaagcac atcctggttc tggccagctt ccgagtcggc gagcagcgcc
      601 cagccgtagc tggaatctgc ttgcacccac cgccgccgcg gcatgagata gtctgcgagc
      661 atactcttga ctcgttcgct cgcctggaca gcagcgagca gctggggctg atccccgaga
      721 atcgcgagga tggcaggatt gtcgcggact ctgcggtggt ggagcagcgc cctacgtatg
      781 cccgaatcga cgggcagctg aagcgtttgt gcgagcacga acagcgcccg ggtcgaggcg
      841 tcacggtcgg cggctaccgc gcggcccatc ccgtggagcg ttcggcgggc cgcatcccga
      901 actgtctcgt acgtgggatc gggaagccca gccatgagcg tcaacagttc cgggtcgccc
      961 tgcactgtcg gggtccggac cagcaggctc gcccgtggct ggtttacgtg ggcatacaga
     1021 tccccgatca agcggaacac cagcgcacgg tacacatggg ggctctcgac ggtcatcggg
     1081 aggaccacct gatcaaccag tgggctttct ccgccgaaga tgggatcgag ggcgtcgagc
     1141 agctgtccgg acaggagata ggagtcgctc tcgtcgatcc ggagcagcgc ctggttcacc
     1201 ctcgacgacg tgcccgccaa gccttcgttc tcatagtcgt agacgtgtag ccagcccagc
     1261 ttgcgtccgt aggccatccc gatcgcattc gaatagaaac aacccgcaag gcaatcctgg
     1321 gcgtgtccgt acacgacgtc gaaaatcaca aggtctcgag tcgtgtcgcg atacagggtt
     1381 cgaatcgctc tctgtagcgg gaaacggaag gcttcgacga actgtcggat gacaggccct
     1441 gccggcgtcg tatcagcctt gggcctggct ggagccggaa ttgcagcctg ctgtctaggc
     1501 tcgggaaagg tgccctcgat cctgaaccac acccggaacc cttgcgtgag cggccaggag
     1561 gcgatcgtga gatgctgcgc gcccgtctgc gcgccaggca acagcctcgt cccgccgttg
     1621 cggccagcaa caaagcgcca cggttgctgt ggctcccggc cttcccagaa tgggaagtcg
     1681 acgtagcggc gagagtagat agcgttcagt tgccggccgc gctgcacggc gaccgcactg
     1741 tccacagtca tcgctatcgg cagagtgatc ggcacattgc tggcgttctc gaccacgacc
     1801 gggacctggt aggcggtcga gaaccaccat gccgaggtcg acgtcattcg ttgcgtgggc
     1861 cccacgatga cgagtttcac ggagtggcac tgtatctcac gaatgcgcac caccaaggtg
     1921 tcaggattgg ggagctgggc ctcgagcacc gcgtcgcagg acgaccgcgg cggagtggcg
     1981 gcaatgccgt tgtcaccctg gcccgtcgga ccgttggcga gcacgatgac gatcccgagg
     2041 attcgctgca tgctcgactc ccgagcaggt ggggctcggc cccgagggtt tccacgcaag
     2101 ccgagcggat tggctaactt agcagatcag taggactggg tgatggggaa caggaactcg
     2161 ggccgtgtcg cgccgcctgg gacaggattc ccaccgcccg ctctatattc cgccttctcg
     2221 tgagtggagt cgctgaatga agacccaggg cagtcaaacc gacatcgtct ttctctcggg
     2281 cgtccgcacg ggcttcggcg cattcggcgg ctccctgaag gagttctccg ccatcgacct
     2341 cgccgcgatc gcggcccgaa acgccctcga gcggtccggc gtcccggccg ccgacatcgg
     2401 gcacgcgata ttcggcaacg cgctccagac ctccgccgac gccatctaca tggcgcgcca
     2461 cgtcgccctc aaggccgggc tcccgatcga gactcccgcc gtcaccgtga accggctgtg
     2521 cggctcgggc ttcgaggcga tcacgcaggg cgcgcagctc atcatcctcg gggaaaccga
     2581 ggcggtgctc gccggcggcg gcgagtccat gagccaggcg ccgcacgtcg tgcgcggcgc
     2641 gcgctggggc ctgcgcctcg gccctgctcc gctggaggat ctcctctggg aggcgctgaa
     2701 ggacccgcag tgcggcttct ccatggccga gaccgccgag aacctcgccg acaagtacaa
     2761 gctcggccgc accgaagtgg accaggtcgc ggtcgactcg caacagcgcg ccaagaaggc
     2821 ctgggacgat tgcgtcttca ccgacgaagt catccccgtc ccggtcaaga agaagggaca
     2881 gaccgtcgag ttccgcgccg acgagcacat gcggccggag acgacgctcg agggtctcgc
     2941 ggcgttgaag ccgtacttca agaaggacgg cgtggtcacc gcgggcaacg cctccggcat
     3001 ctgcgatggg gccgccgcga ctgtgatcgc gagcgaggcg tacgccaagg cccacggcct
     3061 caagccgctg ggacgcctgg tggcctgggc ggtggtgggc gtcgagccca agtacatggg
     3121 gatcggaccc gcgccgtccg cgcgcaaggt gctccagaag gccggcatga cgctcgcgga
     3181 gatggatctg gtggagatca acgaggcgtt cgccccgcag tacctcgcgg tggagaagga
     3241 gctggggctg gaccgcgcga agaccaacat ccatggcggc gccatcgcca tcgggcatcc
     3301 cctcgccgcg agcgggaccc gtatcacgct gcacctgttg cacgcgctgc gccgcacggg
     3361 caggcggttc ggcctgggct cggcctgcat cggcggcggg cagggactcg cggtgatcgt
     3421 ggaagctttc ccggcctgag aggaccgcat gggcgacttc ttccttggta tcatggccgg
     3481 gctcggcctc tatctgttct cgggactgcg cgtgctgcgt gaatacgaac gcgccgtcta
     3541 cttcttcctg ggccgttcct ggggcgccaa aggaccgggc ctgatctatc tgcccccgtt
     3601 gttcgcacgc atgcagaagg tttcgctgcg cgtgatggca ctcgacatcc cgccgcagga
     3661 cgtgatcacc cgcgacaaca tctcgatcaa ggtcaacgcg gtgctgtaca tgctggtgcg
     3721 ggacccggtc aaggcggtga tcggcgtcga gaattacctc tacgccacga gccagctcgc
     3781 ccagaccacc ctgcgttcgg tgctgggcga gacggagctc gacgagctgc tggcgaatcg
     3841 cgagaagatc aacgcgatcc tcaagaagat catcgaccag cgcgccgaag actggggcat
     3901 cgaggtgtcg gcggtggagg tgaaggacgt ggacctgccg ccggagatga agcgggccat
     3961 ggcccggcag gcggaagccg agcgggagcg gcgtgccaag gtcatcaacg ccgagggcga
     4021 gctccaggcg tcggagaagc tggcgcaggc cgcggcggtg atcggccgcg agccggcggc
     4081 gattcagctg cgctacctgc agacggtgac ggagatcgcc tccgagaaca actcgaccac
     4141 gatcttcccg atcccgatcg atattttcag gggcattctc gatgcggtgt cgcggccgcg
     4201 cccggacgag acggccaccc ccgccgcgct cccggcccgc acggcggggg agtccctctc
     4261 cctgccctcc gtccccggca aggacaaggt caaggcgtag gcatggcggg cgagctgatc
     4321 agccgggagg ccctcgaccg catcatccgg cgtgccgccg agctgcagac cggcgagcgc
     4381 gacatcggcg aggggctcac cgaagccgag gtcgaagccc tcggcaacga cgtcggcatc
     4441 ccggcgcgct atctgaagca ggcgctgctc gaggagcgga cgcgccttcc cgacgaagac
     4501 gccgggcggg gactgacctg gtcgctggtg ggaccggggc gcctcaacgc gcaccgcgtg
     4561 gtgccgggag atcgcgccgc cgtggagcgt gcgctctcgg cctggatgga aagcgaagag
     4621 ctgttgcggg tgaagcgtcg ctacaccgag cgcaccacct gggagcagca ggtcgggttc
     4681 atggcgtcga tgcagcgggg cctgcggggc ggcggccgcc gctacgcgct cgcgcaggcc
     4741 gaggaagtcg ccggtcaggt cacccagctc gagccggggt tctgccacgt gcgcctgagc
     4801 gcggacgtgc gcaacctgcg ccgtgcccgg atcggcgggg cggtgacgct cgcggggctg
     4861 ggggcggccg ggtcggtggt cgcggggacc attggggtgc tggcaccatt cctggcgctg
     4921 ccggcggtcg ggttcgccgg gctcgcgctg ctggccctgg tcgccgggcg ccgcacgcag
     4981 ctggggcgga tccaggtggc gctggagcag gtgctcgacc ggctggagcg gggcgagatt
     5041 cgctccgagc acgtcctccc cggcccccgg gccagcgcat tcgtgcggat cgcggaagag
     5101 atccgcaaac aattccagat ctaacgagag atccatgagc gacatcacgc gcgtcggggt
     5161 actggggtgc ggactgatgg gcagcggcat cgcgcaggtc gccgccaccg cggggtacga
     5221 gacgatcgtg cgcgacgtag cgcaaccgat cctcgacaag gcgaaggcgg gcatcgagaa
     5281 gtctctcgcc aagtttgtgg agaagggtaa gctccaggcg ccggaccggg acgccgcgct
     5341 ccggcgcctg tcgttcacga cggtgaccgc cgacctgaag gcgtgcgacg tcgtggtgga
     5401 ggccgtcacc gaggacctcg aggtcaagaa cgcgctgtgg agagagctgg acgccctgtg
     5461 ccccgccggg acgatcttcg cctccaacac ctcgagcctc acgatcgccg cgatggcggc
     5521 ggccaccaaa cgcgccgacc ggttcgtggg cctgcacttc ttcaacccgg tgccgctgat
     5581 gcagctggtg gaggtggtac gcacggtcac gacgtcggag gacgcgttca agcgggcctt
     5641 cgccttcgcc cgctcgctcg gcaaggagcc ggtcgcggcg aaggacaact ccgggttcat
     5701 cgtgaacctg ctgctcgtgc cctacctgct cgacgcgatc cgggcgctgg aacacggcgt
     5761 ggggagcgtg accgacatcg acaaggccat gcagctcggg tgcgggtacc ccatgggacc
     5821 gctcacgctg ctcgacttcg tggggctcga cacgacctac aagatcgcgg aaatcatgtt
     5881 cgcggagtac aaggagcaac ggtatgcgcc gccgccgctg ctcaaacgca tggtgctcgc
     5941 cggcatgtac ggtaagaaga gcggcaaggg gttctacgac tattcggcca atccaccggt
     6001 agtctcgagc ctgggactct aacccgaccc agaggagtac agacccgcat gtttggctac
     6061 gacctgccgc gcctgcacgc ggcggtgaac gaccttcccg cggcgttgct gctggccgcg
     6121 gtcctgttcg atctgatggg atggattctg aagcgggact cgctcgtgtg ggccggcatc
     6181 tggacgctct gggccggcgt catcggcggc tgggcggcgg tgatcctggg cgagctcgcc
     6241 gaggacgccg tcgaacacgg cgatgcggtc catgcgatta tggccacgca cgagaagctc
     6301 gccctcatca cgatgagcct gttcaccgtc gtgctcgcct ggaagctgtt ccgccgcttc
     6361 cagctgcgac ccctcgaagc gcgcttgctc accctgctcg cggccgtggg gttcgtcttc
     6421 ctgctctgga ccggcgcgga aggcggcgag atggtgttcg accacgccgc cggcatttca
     6481 accgcgaagc tccgggcgga gatcgagaac cgggaggccg gccaccatca cggtcccggt
     6541 gaggccgatg accacgatca cgacgcggcg gattcggccc acgactcggc tgccggcacg
     6601 cacgtcgatc ctccgggcac gcccccgcac acgcactgac atgcgattgc ggattgcgga
     6661 ttgcggattt tggattgtca ggcgtggagc aggcgcccgc agggcgactt gggccgaccg
     6721 cgtcaaatcc gcaatccgca atccgcaatc cgcaatcgtc ctggttctcg ccgcctgctc
     6781 ccccgtcacc acccggcccc cgttcctccc ctacccgcag gcgctcgtca ccgtgctcga
     6841 cgcgccgccg ggccgcgtcg ttcccgaagc cctgggctgg ttgacgagcc agggcctgca
     6901 tgtccagtgg tcgagcccgc aagatggcta cgtcgagtcc gcgtggttca acctgcgcac
     6961 gcacacttcg gtgttcggcg atgccgatcc gggcgatctc ctcttcaccg tgaagatccg
     7021 ctgctgggcg gatcccaaca tccccggcaa gacccagctc accgtcgaag cggtgtaccg
     7081 ccccgtgctc gacccctcgc gacccgaacg cgacctcgag gagatcgtcc cgcagggatc
     7141 ggaaggctac cgcatcgccc agcagctgat cgacgcgctg aagcagaaat tcgggaccta
     7201 accgccctca gccgacacgg tcgccagcca ccccagcccg tcgagcatca cccctccgct
     7261 gcggcatcga ggccgaccac gccgctctcg gcctcgcggc ggaacgtcaa ccaatcggtc
     7321 cgcacccggt cccactcctg gatcacgagc ccatggccct cgaacagggc ccggatctgg
     7381 ctcggtcccc aggccagaat ctcctcgagg atcggcacga gcacgctgag gacgtcctcg
     7441 ctgatcacgg tacgcttcgc aatctgtgcg atctcgcgcg cctccgacgc cgcgatggtg
     7501 aacccatcac cacggttgac gtgcagcatg accgggatgt ccgactggcc atccccgaca
     7561 tacaccacct ggtcgcgcgg cagcgcgagc tgcaggcgcc gctcctcgac cgccgccacc
     7621 ttgccgaagc cggcggccac gcgagccacc gactcgatct cgcccgaccc ggtgtcccag
     7681 ccgaactgcg tcccgacaat gtgatccggc gggacgatcc catcgagcgc tgcgagcacc
     7741 acctcttcgg gtgccgccga gatcacgtag aaatcgaagc ggtggccatc gatgcctcga
     7801 gccagcagcg tcgtaagtaa ccgcacgttt cgcttcagcc gcacacgccg cccgacttcc
     7861 accagatcgg cctgtcgtac ccgacgatac tcgggatcgt gccgcagcag ataggcgagc
     7921 tcggcaccct gctgcaccag attcaagcgc gccagcgccg ccactttctc ggggaaaccg
     7981 gaagtcccga gcaactcgct caggatgatg ccggaatcgt tgaagctcag cgtctggtcg
     8041 aaatcacttg ccaggagata gtgcttcttc atcgagattc ctcgtcaatg aaaaagcccg
     8101 ctcccttgaa gcggagcggg ccggtctgtc gtgcgacgca ttcgattcgc gtcaggcggt
     8161 acgcgcgctc ccggggctgc ggggcccggt gaaatggagc aggttcatga gacagcgcgt
     8221 catggcggaa ttgtacccac gacgcgctgc gagggaaagg gcaaagacca gcaggaagaa
     8281 cggtgcgatg gtattcatgg gtgctatgtt ccccccacgt gacctcgacc cagaccgacc
     8341 cacgtatccg ctccgcctac gccgaagggg ccgggatcta ccgcatcctc ccggaggcgg
     8401 tagcgattcc ccgcactgtc gacgacgtgg tgcacctggt gcgcgacgcc gagcgcgggg
     8461 tcaccccgct catcccgcgc ggtgccggca gcggcatgcc cggcggcaac gtgggccgag
     8521 gcaccatcgt tgactgctcg caggggttcc gcgtgctgga gctggatccc gctcggcgga
     8581 tcgcacgcgc cggcgccggg gtcacctggg cggaggtgaa cgacgccgcg cgaccgcacg
     8641 ggctgcgcct cccccccgat ccctcctccg gcgccttcgc cacctcgggg ggcatggtcg
     8701 cgaccaacgc cgcaggcccg cgctcggtgc gctacgggag cgtgcgcccc tggatcgagg
     8761 ctctcgagct cgtcggcaac ggaggagaga tccgcactat ccgtcggggt ggcggcgcgg
     8821 ggccctggcc cctgaccccg gacacccggc acctcgtcgg gcaacgcttc ccccacacga
     8881 ggaagaactc gtcgggctac gccctcgacg ccttcgctgc ctcgggggac gagctggacc
     8941 tctacatcgg ggccgaggga acgctgggca tcgtgacgac cgtcgaatgg cgcctcgatc
     9001 ccattccccc cgaatcggcc ggcgcggccc tgacgctcgg cagcctggac gacctgggag
     9061 aggccgtcgc ctatctggcg accctcgatc cggccgccgt ggagctgttg gacaagacgc
     9121 tgctcgagtt cgtagccgcg gcgggcggcg aggtccatcc gggcgccgag tgcctgctcc
     9181 tggtcgagtt cgagcgccag acggccgccg ccgcccgcgg ggtcgtcggg gacgcggtgc
     9241 gcggcctgaa gcacctctcc atgcaggccg ataccgcgct cgaccgtgcc ggcctggaac
     9301 ggatctgggg aatccggcgc ctggcgagtc cggccctggc gcgtcttccg gagacccgcc
     9361 gctcgctgca ggtggtcgag gacgggtgtg tgccgactgc gcgactgagc ggctacctgg
     9421 cagggctccg ggcggcggcg gcgcgccacg agatccctgt cgccatcttc ggccacgcgg
     9481 gcgacgggca tgtgcacgtc aacgcgctcc ccgatctcac gcgcccgggc tggcgcgacg
     9541 cgttgcaggc cctctacgag gaggtgcagg atctcctcgt gaacctcggc gggacgccga
     9601 gcggcgagca tggcgacgga cggctgcgcg gcggcacgct cgagcggttc ttcggc
//
LOCUS       DBNGZW010000438         1852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_586409, whole genome shotgun sequence.
ACCESSION   DBNGZW010000438 DBNGZW010000000
VERSION     DBNGZW010000438.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1852
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_586409"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..719)
                     /locus_tag="AC16JC_10385"
     CDS             complement(<1..719)
                     /locus_tag="AC16JC_10385"
                     /inference="COORDINATES: protein motif:HMM:NF016424.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HIR5165142.1"
                     /translation="MLFLLAQLQVDSLPPPRGFVNDFAGVIPAATVARLEPMLLDLQR
                     KTHGDIAVVTLPDLGGRAPEEVARQLGRQWKVGGSGEAGDPARNLGVVVLVVPGSRDL
                     FIATGRGAEGFLPDARVGRIRDAMTPYLAQGDYGRGLEVGVGLLVRAFTDEFHVTLDS
                     SLAVAAPSGQEEGRPLPGIAIGIIAVVIALVMFLNMAARAARVGRRRGGWWSGGGWGG
                     WGGGGWSGGGFGGFGGFGGGGG"
     gene            834..1280
                     /locus_tag="AC16JC_10390"
     CDS             834..1280
                     /locus_tag="AC16JC_10390"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR5165143.1"
                     /translation="MPRLQLLAALMAGASLAAACGHTTSVARDDVLVAPDGRRIITEK
                     AIEHSGARTAWDALQRTVPFFNFNDGTRGRPARIEHRGRSSIVLRDQPLILVDGVELN
                     DFTVLGGMPAADLYEIEVLTGIDATTYYGTNATKGVIRIWTKSGAT"
     gene            complement(1364..>1852)
                     /locus_tag="AC16JC_10395"
     CDS             complement(1364..>1852)
                     /locus_tag="AC16JC_10395"
                     /inference="COORDINATES: protein motif:HMM:NF019592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HIR5165144.1"
                     /translation="PRVVSVLRGKGFDNVSPLYVLLKYTSATSPSRVADGIEARLIAA
                     EAQLKAGDGAGMVTSLNALRADNANNGGFTLVALPDPGTQAGREDLLFRERAFWLFAT
                     GHRLGDLRRLARQYGRAVNTVYPTGAYLHASTVFGSNTELPVPFNERNNPNFTGCDYN
                     TP"
BASE COUNT          296 a          701 c          603 g          252 t
ORIGIN      
        1 ccgcccccgc ccccgaaccc gccgaagccg ccgaacccgc cgcccgacca gccgccccca
       61 ccccacccac cccatccccc accgctccac caaccgccgc gacgacggcc gacccgcgcc
      121 gctcgcgcgg ccatattgag gaacatgacc agcgcgatca ccaccgcgat gatcccgatg
      181 gcgatccccg gcaacggacg tccttcctcc tgcccggacg gcgccgccac ggcgagcgag
      241 ctatccagcg tcacgtgaaa ctcgtcggtg aacgcccgga cgaggaggcc gacgccgacc
      301 tccagaccgc gcccgtagtc tccctgggcg aggtagggcg tcatcgcatc ccgaatgcgc
      361 cccacgcggg catcggggag gaacccctcg gcgccccgcc cggtggcgat gaacaggtcc
      421 cggctgccgg gcacgacgag caccacgacg cccaggttcc gcgccgggtc tcccgcctcc
      481 ccgctgcccc cgaccttcca ctggcggccg agctgccgcg ccacctcttc cggcgcacgc
      541 ccgcccaggt ccggcagggt cacgacggcg atgtccccat gggtcttgcg ctgcaggtcg
      601 agcagcatcg gctcgagacg cgccacggtg gcggccggaa tcacaccggc aaaatcgttg
      661 acgaagccgc gtggcggtgg gagggaatcc acctggagct gcgcaagcag aaacagcacg
      721 agcggttgca tgggcccgga cgaaaggtag cacgattcgg acgccctgcc tgcttgacga
      781 ggacctcgtt tccgctagca tgatttgtat ccaccggacc tgcgaggtcc cgcatgcccc
      841 gtctgcagtt gctcgccgcg ctcatggccg gcgccagcct ggccgcagcc tgtggccaca
      901 ccaccagcgt cgcccgtgac gacgtgctcg tggcgcccga tgggcggcgc atcatcacgg
      961 aaaaggccat cgagcattcg ggcgcgcgga cggcgtggga cgccctgcag cgcaccgtcc
     1021 cgttcttcaa cttcaacgac ggcacgcgcg gccgcccggc gcgcatcgag catcgcggcc
     1081 gctcgagcat cgtgctgcgg gaccagccgc tgatcctggt ggacggcgtc gagctgaatg
     1141 atttcaccgt cctgggcggc atgcccgcgg cggatctgta cgagatcgag gtgctcaccg
     1201 gcatcgacgc cacgacctac tacgggacca acgccaccaa gggcgtgatc cggatctgga
     1261 ccaagagcgg cgcgacctga gcgcgtcgcg ggcctggtgc aacgcggggg agtcgagctt
     1321 ccgactcccc cgcgcgtgcg ttcaggaacc gcgcttcccc cgactacggg gtgttgtagt
     1381 cacagcccgt gaagttcggg ttgttgcgct cgttgaacgg caccggcagc tcggtgttcg
     1441 agccgaacac cgtgctggcg tgcaggtagg cgcccgtcgg atagaccgtg ttgaccgccc
     1501 gcccgtactg ccgggcgaga cggcgcaggt cacccagtcg gtgaccggtc gcgaacagcc
     1561 agaacgcccg ctcgcggaac agcaggtcct cgcgcccggc ctgcgtgccc ggatcgggca
     1621 gtgccaccag ggtgaagccg ccgttgttcg cgttatcggc tcgcagcgca ttcaggctgg
     1681 tcaccatccc cgcgccgtcc ccggccttga gctgcgcctc ggcggcgatg agacgcgcct
     1741 cgatgccgtc cgccacgcga ctggggctcg tcgcagaggt gtacttgagc agcacgtaca
     1801 gcggcgacac gttgtcgaac cccttgccgc ggagcaccga gacgacccgc gg
//
LOCUS       DBNGZW010000439        11368 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1675985, whole genome shotgun sequence.
ACCESSION   DBNGZW010000439 DBNGZW010000000
VERSION     DBNGZW010000439.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11368
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1675985"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..721)
                     /locus_tag="AC16JC_10400"
     CDS             complement(<1..721)
                     /locus_tag="AC16JC_10400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide reductase N-terminal alpha
                     domain-containing protein"
                     /protein_id="HIR5165145.1"
                     /translation="MTASRTKGTLDLSANALTVLERRYLVKDDQGKPVERAEDLFWRV
                     ARTIAAPDRVFGASDKAVETVAETFFELMATRVWMPNSPTLMNAGRPLGQLSACFVLP
                     VDDALSNGKSGIYDTLRAMALVHQSGGGTGFAFSRLRPQNDVVRSTMGVASGPVSFMK
                     LYDASTDVVKQGGTRRGANMGILRVDHPDIMEFITCKDDLTQVTNFNISVAVTDAFMA
                     AVEQGRPDDLIHPRSGTAVGQL"
     gene            complement(939..1400)
                     /locus_tag="AC16JC_10405"
     CDS             complement(939..1400)
                     /locus_tag="AC16JC_10405"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018571149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HIR5165146.1"
                     /translation="MGDVTLGPRASVWYGAVLRGDMDRIVVGADSNLQDGTIVHVDEG
                     RPALIGDRVGVGHRAIVHGCTVEDDCLIGMGSVLLNGAVVCAGSVVAAGAVVREGMVI
                     PPGSLVAGVPAKVLRPVDDGLRDRIRATWRHYVELAQRHRTGAFPRSPAGL"
     gene            complement(1445..3385)
                     /locus_tag="AC16JC_10410"
     CDS             complement(1445..3385)
                     /locus_tag="AC16JC_10410"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5165147.1"
                     /translation="MYCPHCGAQNEESFKFCKTCGADLSATLPLDAVVKPSLGPPPAD
                     AAEPSELDLVRAELEEEYEIQEELGRGGMAIVFRAKEKQLDREVAIKVLPFSLAFDKE
                     FVERFQREARLSARLEHPNIIPIYRVGKSGRVIFFVMKFLRGKPLSALLAVRGALPPM
                     EIRQVLIQVGRALAYAHRSGIVHRDIKPDNIMFDEHGQALVTDFGIAKAGTTSKLTGT
                     GMAIGTPHYMSPEQARAQALDGRSDIYSLGVVAYQCLTGLVPYDGEDSFSIGYKHIME
                     ELPAPVLENEEQRDLFEVIRKMMAKLPAERFQTADEMVEVLESGAGFVATGISTAQTR
                     AMPSLGGGMPKLSTAPTTPLPRVSAGLPPVPQGPQPRSAVAGLLIWLLVMGAVLGGGG
                     YLAYKKGLIFAQAPADTTALARADSLQAAGDSGAGLRADSMAGGVGDSIIRTAGSGGT
                     GGPAVAPGTPGRLVLQGAFPPDARVTMDGQAVQGNQLELPPGTHKLAVRAAGYVTYER
                     QVLVAPGGTSTVPITLQKSRLVGDTMAPPGGGTVSSCDQFGPTYNQDNACFDTRPAPL
                     TAPYVLLTPDATETPRLAILLVKVSREGETLEARVFVGSNVTTFTNAALDLAKRLRWN
                     PAQKNGEAVDAWTQLQVLPQRP"
     gene            complement(3448..4299)
                     /locus_tag="AC16JC_10415"
     CDS             complement(3448..4299)
                     /locus_tag="AC16JC_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165148.1"
                     /translation="MPMNVVVHDFVAGAGTAPPESAPSAAGGDAPPPTGSAAGQALHV
                     VFAIPAERLGPEPGGEGVLYPLHFRLLVSDPHDSLIARLDTVRVFTARQALRAPSFLS
                     GRLSLTLAPGRYRYRLLVTSTDGLAGQLVVRDTLEVPALDGRAFATSDVVVGRAGAGL
                     VWLPPRDTVLLDPLGRYPSGSVAELYYEIYGLTAGESYHTEVRLERQGGRSLLGRIGG
                     LFGGRRPPVLLAFDSPADGPITRVHRGISLRDVATGSYLLTVTLSIPGSDRRVSRSEP
                     LQVVSGP"
     gene            complement(4315..5784)
                     /locus_tag="AC16JC_10420"
     CDS             complement(4315..5784)
                     /locus_tag="AC16JC_10420"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HIR5165149.1"
                     /translation="MRSSLLPNALVCAAISVGLATAPLAAQEPDARAAIERLRDSLAG
                     VTDSLALHRLEAATIEVAKRDRDNPVIHLRLGFIAYRLGELAKAKGHYDDAAGEFEWA
                     SQLRPEWPYPWYGLGLAELAQGEHSAIAIENLRQQLGLDYLSKAARAFARATQADPGF
                     AFAAIDLATAALAQRIHPRLQVALEAVRLAAASPAGTNPAVQLARGRVEREVGEADSA
                     LAAFHGYLAVGGDSGIGLFELARTQFFANQPAAAGSSYYAGARAATSAPARAQYRQDL
                     AYLADSAELQDFDAQPDADACVAWLRRFWNRRDVVEGRDMGERLAEHNRRWFYAERNF
                     RLVSRHRHYDITEVYRSGQVVFDDRGIIYLRHGEPDERAIFPSDPGCAAGEQCVQPNT
                     SWLYHRPGGDIVFHFVARDDAADFKLVESLVDAMGFRAAVQAAGHPDQKVIDLYASRE
                     QFGGTYARVARGGSLVGTVLSDERSAGRRTVTSGTTTDT"
     gene            complement(6024..7223)
                     /locus_tag="AC16JC_10425"
     CDS             complement(6024..7223)
                     /locus_tag="AC16JC_10425"
                     /inference="COORDINATES: protein motif:HMM:NF014428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5165150.1"
                     /translation="MNLFPRHFGRLATVTALWALSGCGGDSGGPVTPRTLAVTGGNQQ
                     SGCLVDTLPDSLEVTLTGSDNKPFAGAPVAWQVTGGAATLSRALDTTDAAGRSRIQLT
                     LGIALSPVAVSATVTGVSPATFIASGRIAAYALGQSVSGALTPGDCAGSDTPSFDLYA
                     LTLPGPQSFTASLSSATFDAVLVAGDTLSLMAFNDDSGDGSGGTDSFFRMIATGGFYV
                     IEAQSFSAGETGPYTLTTVATPVAVDNCPFDVFVTRGITTDQDIKATDCADSSGPFYY
                     DVYEMFLDAGQTMTVTESSTAFDAELFLFVGSTLVGSDDNSAGGTNARVTHQAPLGQN
                     QLVHIVPSTKGTGVTGAYTLAIAPPAAATALAARMFAPQTDRLVDPFGTSKARAASLR
                     EWHRLRQ"
     gene            7437..10046
                     /locus_tag="AC16JC_10430"
     CDS             7437..10046
                     /locus_tag="AC16JC_10430"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5165151.1"
                     /translation="MSPSLQAALAGRYAIERELGHGGMATVYLARDLKYARPVALTVL
                     RPELTAALGAERFLREVRISARLDHPHILTLIDSGESDGFLWYVLPFVHGESLREALK
                     RERQLELDRALAITRQIAGALDYAPRQGVIHRDIKPENILLHEGEAMLADFGIAMAVR
                     EAGGSRLTESGQSLGTPQYMSQEQATGERELDARSDVYSLGAVLYEMLTGEAPHTGAT
                     MQAVIAKLMTERPTRVRTVRDTVPEGVDNAVAKALAKVPADRFHGAAEFAEALDVRGA
                     VARGTLPRSLKWALGTAGLVTVAAVLVILSRLRQESIALGNVSQMTFEPGLEIEPAIS
                     PDGKLVAYAGGPLSQTRIYVRQPGGRPVALTADSGPPQRRPLWSPNGTRILFETDEDL
                     FLVPLFGGVPRLMARRACCAAWSPDGREVAYVGSPRRPGSFERPESLFVRPPDGGASR
                     FVNVAFDPHSLAWSPDGKWLALVSGNDQFAVGTALFGNTGPSAIVLVPAAGGAQVPVT
                     DNTKLNVSPVWTPDGHHLLFVSDREGARDVYAVRLGSSGAPAGPPSRITTGVNAHSIS
                     LSASAGRLAYSVYTSRANVWTIAIPTDGPVSVDGAIQITTGNQTVEQMAVSPDGRWLY
                     FDSNRFGNADIYRMPVDGGEPEQLTTDPADDFGPDVSPDGRWMAFYSMHLGTRDIFVM
                     PAEGGEMERVTDDPGEERIPSWSPDGRSLSYFLNGTGARDGLYVISRDASGRWGRPRQ
                     VWNHPDVGRWSPDGQTLVSTWTDGVWLIPVAGGRPQRASPFPTLTIPEQVWAYWSRDG
                     RTIYFKARDRAGRSSIWGVAAAGGHPHTLVRFDDPARPSYYTSWASDGRRFYFTINDR
                     QSDIWIAELNGLK"
     gene            complement(10264..>11368)
                     /locus_tag="AC16JC_10435"
     CDS             complement(10264..>11368)
                     /locus_tag="AC16JC_10435"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HIR5165152.1"
                     /translation="VLDDDTAHSGTTDSLTVGKAVPGGTYQWFFPTGTTAAVAGWLNG
                     EVRLALSRSAAAWVNAADVHELPAGTPPPAGTVGSVRLHPESGWVTLRVPLPARLPYR
                     VEEGDRSLTLRIYGGASDVNWMQYGGTDPLVTRMSYRQESVDELTITLELSRRVWGYR
                     SRFDGRDLLLDVRRPPAIDARHPLRGRTIVLDPGHPPVGATGPTRLWEPVAVLAVAQK
                     AERLLARAGATVLLTRSDMTPVDLYPRTRFAEQRNADVLVSIHANALPDGVNPFVNNG
                     TSVYYFQPRSADLARALDRALVRALGTRDLGMGRGDYALVRPTWMPAALTEGLFIMLP
                     EQEAMLRSEDGQWRYARAVAEGIETFLRERARD"
BASE COUNT         1763 a         3992 c         3896 g         1717 t
ORIGIN      
        1 cgagctggcc caccgccgtc ccgctgcgcg ggtggatcag gtcgtcgggc cgcccctgct
       61 ccaccgccgc cataaaggcg tcggtgaccg cgaccgagat gttgaagttg gtcacctgcg
      121 tgaggtcgtc cttgcaggtg atgaattcca tgatgtcggg atggtccacg cgcaggatgc
      181 ccatgttggc gccgcggcgc gtgccgccct gcttcacgac gtcggtggag gcgtcgtaca
      241 gcttcatgaa cgacaccggg cccgacgcca cgcccatggt cgagcgcacc acgtcgttct
      301 ggggccgcag ccgcgagaag gcgaagccgg tgccgccgcc cgactggtgc accagcgcca
      361 tcgctcgcaa cgtgtcgtag atgccgctct tgccgttgga gagcgcgtcg tccaccggca
      421 acacgaaaca ggccgacagc tgacccagcg gccggccggc gttcatcagg gtcggcgaat
      481 tgggcatcca gacgcgggtg gccatcagct cgaagaacgt ctcggccacc gtctcgacgg
      541 ccttgtcgga cgcgccgaag acgcggtcgg gtgcggcgat ggtgcgcgcc acccgccaga
      601 acaggtcctc ggcgcgctcg accggcttgc cctggtcgtc cttgaccagg tagcggcgct
      661 ccaggaccgt cagggcattg gcactcaggt cgagagtgcc cttggtgcgg gaagcagtca
      721 tatgcggctc gtccccttcg atcggacggt ggcaggtcgg cgaccaatgc ctcgttgggc
      781 tcccgatggg agccgggtac gggactggtc ctggcgttga aggtaactag acggccgtgg
      841 acttcttgaa gacgcggagg gccaaggtag gtacgcccgc ccgcgcgggc aagttttcgc
      901 gaaaatcccc ggacgaccgg cgttctcccg tttccacatc acaaacctgc tggactgcgc
      961 gggaaggccc ccgtgcggtg acgctgcgcc agttccacat agtgacgcca ggtggcccgg
     1021 atccggtcgc gcagcccgtc gtccaccggc cggagcacct tcgccggcac cccggccacc
     1081 agggaccccg ggggaatcac catcccctcg cgcaccaccg cccctgcggc cacgacgctg
     1141 cccgcgcaca ccacggcgcc gttcaacagc acgctgccca tgccgatgag acagtcgtcc
     1201 tccaccgtgc agccgtgcac gatcgcccgg tgccccacgc ccacgcggtc gccgatcagc
     1261 gccggccgcc cctcgtccac gtggacgatg gtcccgtcct ggaggttcga gtccgcgccc
     1321 accacgatgc ggtccatgtc gccgcgcagc acggccccgt accacacgct ggcccgcggc
     1381 cccagggtga cgtcccccat cacccaggcc ccgggcgcga tgaacgcctc cggatcgatg
     1441 tgaatcaggg cctctgcggc aggacctgca gctgcgtcca ggcgtcgacc gcttcgccgt
     1501 tcttctgcgc cggattccag cgcagtcgct tggccaggtc gagggcggcg ttggtgaacg
     1561 tggtcacgtt ggagcccacg aatacccgcg cctccagcgt ctcgccctcc cgcgacacct
     1621 tcaccaacag gatcgcgagc cgtggggttt ccgtcgcgtc cggggtgagc aacacgtagg
     1681 gtgccgtgag cggcgcgggc cgcgtgtcga agcacgcgtt gtcctggttg taggtcggcc
     1741 cgaactggtc gcacgacgaa acggtgccgc cgccgggcgg cgccatcgtg tcgccgacca
     1801 gcctggactt ctgcagggtg atcgggacgg tggacgtgcc gccgggcgcc accagcacct
     1861 gacgctcgta ggtgacgtac ccggccgcgc gcaccgccag cttgtgcgtg ccgggcggca
     1921 gctcgagctg attgccctgg accgcctgcc catccatcgt gacgcgcgcg tcgggcggga
     1981 atgcaccctg caggacgaga cggcccggcg tcccgggcgc caccgccggg ccccccgttc
     2041 cccccgaccc cgccgtcctg atgatgctgt cgccgacgcc gcccgccatg gagtccgcac
     2101 gcaatccggc gccggagtca cccgctgcct gcagcgagtc ggcgcgcgcg agcgcggtcg
     2161 tgtcggccgg ggcctgtgcg aagatcagac ccttcttgta ggcgaggtac ccgccgcccc
     2221 cgagcaccgc gcccatgacg agcagccaga tcagcagtcc cgcgacggcg gagcgcggtt
     2281 gcgggccttg cggtaccggc ggcaacccgg cgctgacgcg cggcaggggc gtggtgggcg
     2341 cggtggagag cttgggcatt cctccaccga gcgacggcat ggcccgcgtc tgggccgtgc
     2401 tgatgccggt ggcgacgaac cccgcgccgc tctccagcac ctcgaccatc tcgtcggccg
     2461 tctggaagcg ctccgccgga agcttggcca tcatcttccg gatcacctcg aagagatccc
     2521 gttgctcctc gttctccagc accggggcgg gcagctcctc catgatgtgc ttgtagccga
     2581 tggagaagga atcctcgccg tcgtagggca ccaggccggt gagacactga taggccacga
     2641 cgccgagcga gtagatgtcg ctgcgcccgt cgagcgcctg ggcccgcgcc tgctcgggac
     2701 tcatgtagtg cggcgtcccg atggccatgc ccgtgcccgt gagcttcgag gtggtgcccg
     2761 ccttggcgat cccgaaatcg gtgacgagcg cctggccgtg ctcgtcgaac atgatgttgt
     2821 cgggcttgat gtcgcggtgc acgatccccg agcggtgcgc gtaggcgagg gcgcggccca
     2881 cctggatcag aacctggcgg atttccatcg gcggcaacgc cccgcgcacg gccagcagcg
     2941 ccgacagcgg cttgccgcgc aggaacttca tgacgaagaa gatcacccgt cccgatttgc
     3001 cgacgcgata gatcgggatg atgttcggat gctcgagccg cgccgacagg cgggcctcgc
     3061 gctggaagcg ctcgacgaac tccttgtcga acgccagcga gaacggcagg accttgatgg
     3121 cgacctcgcg gtccagctgc ttctccttgg cgcggaacac gatcgccatg cccccccgcc
     3181 ccagctcctc ctggatctcg tattcctctt ccagctcggc ccgcaccaga tcgagctccg
     3241 atggctccgc ggcatccgcg gggggcggcc cgagcgacgg cttcacgacg gcatcgagcg
     3301 gcaacgtcgc ggagagatcg gcgccgcagg tcttgcagaa cttgaaggac tcttcgttct
     3361 gggcgccgca atgcgggcaa tacatcacta catcacatct ccggggggaa ccgcggaaac
     3421 ttagggacct gcgcgcgaac gtgtcaatta cggcccggac acgacctgca gcggctcgct
     3481 ccggctcacg cgccggtccg atccaggaat gctcaaggtg acggtgagga ggtagctccc
     3541 ggtcgcgacg tcgcgcaggc tgatgccgcg gtgcacgcgg gtgatcggcc cgtcggccgg
     3601 cgagtcgaac gcgagcaata ccggaggccg gcggccgccg aacaggccgc cgatcctgcc
     3661 cagcagcgag cgcccgccct gccgctccag ccgcacttcg gtgtggtagg attccccggc
     3721 cgtgagccca tagatctcgt agtacaactc cgccacgctc cccgacggat agcgccccag
     3781 cggatcgagc agcaccgtgt cgcgcggcgg cagccacacg aggccggccc ccgcccggcc
     3841 caccacgacg tcgctggtcg cgaacgcgcg gccgtcgagc gcgggcacct ccagcgtatc
     3901 gcgcaccacc agctgccccg ccagcccatc ggtggaggtg accagcaggc ggtagcggta
     3961 gcgtcccggc gcgagcgtga gactcaggcg gccgctcagg aaactcggcg cgcgcagcgc
     4021 ctgccgcgcc gtgaagacgc gcaccgtgtc gagccgggcg atcagactgt catgcgggtc
     4081 cgagacgagc agccggaagt gcagcgggta aagcacgcct tccccgcccg gctcgggacc
     4141 gagtcgctcg gccgggatgg cgaacaccac atgcagcgcc tgccccgcgg cggacccggt
     4201 gggaggcggc gcgtcgccgc ccgcggccga cggcgccgac tcgggtggag ccgtgccggc
     4261 gccggccacg aaatcgtgca ccaccacgtt catcggcacc gggaagcgct gcgcttaggt
     4321 gtccgtcgtc gtgcccgacg tgaccgtgcg ccgtcccgcc gagcgctcgt cggagagcac
     4381 cgtgccgacc agcgaccccc cgcgcgccac ccgcgcgtag gtgcccccga actgctcgcg
     4441 cgacgcgtag aggtcgatga ccttctggtc cggatgcccc gcggcctgca cggcggcgcg
     4501 gaaacccatc gcgtccacca ggctctcgac cagcttgaag tcggcggcgt cgtcgcgcgc
     4561 cacgaaatgg aagacgatgt cgccgcccgg gcgatgatac aaccagctcg tgttgggttg
     4621 tacgcactgc tccccggccg cgcatcccgg atccgacgga aagatggcgc gctcgtccgg
     4681 ctcgccgtgc cgcagataga tgatgccccg gtcgtcgaac accacctgtc ccgaccggta
     4741 cacctcggtg atgtcgtagt ggcggtgccg gctcaccaga cggaagttgc gctcggcgta
     4801 gaaccagcgc cggttgtgct cggcgagccg ctcgcccatg tcgcggccct cgacgacgtc
     4861 ccggcggttc cagaagcgcc gcagccaggc gacgcacgcg tccgcatcgg gctgcgcgtc
     4921 gaagtcctgc agctcggcgg aatccgccag gtaggcaaga tcctggcgat actgcgcgcg
     4981 cgcgggagcg ctggtcgccg cccgcgcgcc cgcgtagtag ctcgacccgg ccgccgcggg
     5041 ctgattcgcg aagaattgcg tgcgggcgag ctcgaacagg ccgatgccgg agtcgccgcc
     5101 caccgcgagg tagccgtgga acgcggccag cgcggagtcc gcctcgccga cctcccgttc
     5161 cacccgcccg cgggccagtt gcacggccgg gttggtcccc gcgggcgacg ccgccgcgag
     5221 ccgtaccgcc tccagcgcca cctggagccg cgggtgaatc cgctgcgcca gcgcggccgt
     5281 cgcgagatcg atggccgcga aggcgaagcc ggggtccgcc tgggtcgcgc gtgcgaaggc
     5341 gcgggccgcc ttggagaggt agtcgaggcc gagctgctgt ctcaggttct cgatcgcgat
     5401 cgccgagtgc tcgccctgcg ccagctcggc gaggccgagt ccgtaccagg gatacggcca
     5461 ctcgggccgc agctgggagg cccactcgaa ctcgcccgcg gcatcgtcgt agtgcccctt
     5521 cgccttggcg agctcgccca aacggtaggc gatgaagccc aggcgcaggt ggatgacggg
     5581 gttgtcgcga tcgcgcttgg cgacctcgat ggtggccgcc tcgagccggt gcagggccag
     5641 cgaatcggtc acgccggcga gcgagtcgcg cagccgctcg atcgcggcgc gcgcatcggg
     5701 ctcctgggcc gccagcggcg cggtggcgag ccccacgctg atcgcggcac aaaccagcgc
     5761 gttggggagg agggacgacc tcatgggata ttccattata cagcggcccc acggcgggga
     5821 ttcattacag ttcgatgacg gcgtggtggc cgggagcctg gagccttggc ttgaagccgc
     5881 ccggcccccg tcgcggtcgc tgtgaccgtg acgggggcag tgtgataaga aggaaacgtc
     5941 aggtcagggc taccagcact cgctccggaa atcttcttcg accccgacca acgtccccgc
     6001 tgggattcac cgtacagtgc gcgttactgc ctcagccgat gccactcccg cagcgatgcc
     6061 gccctcgcct tggaagtgcc aaacggatcg accagccgat cggtttgcgg cgcgaacatt
     6121 cgcgctgcca gggccgtcgc cgcggcgggc ggcgcgatcg ccagggtgta cgccccggtt
     6181 acgccggttc cctttgtgct cggcacgatg tggacgagct ggttctggcc caacggggcc
     6241 tgatgggtga cccgggcatt ggtgccgcca gcgctgttat cgtcggaccc tacgagcgta
     6301 ctccccacga agaggaagag ctccgcgtca aacgcggtcg agctctccgt gacggtcatg
     6361 gtttgccctg catcgagaaa catctcgtac acgtcgtaat agaagggccc gctcgagtct
     6421 gcgcagtcgg tcgccttgat atcctggtct gtggtgatgc cgcgggtcac gaatacgtcg
     6481 aaggggcagt tgtccaccgc cactggggtc gccacggtcg tgagcgtgta gggcccggtc
     6541 tcccccgcac tgaaggactg tgcttcgatt acgtagaacc caccagtcgc gatcatcctg
     6601 aaaaaggaat ccgtcccccc gctgccgtcg ccgctgtcgt cattgaatgc catgagcgat
     6661 agcgtgtctc ccgctaccag caccgcgtcg aatgtcgcag agctgagcga ggccgtgaag
     6721 ctctggggcc ccgggagagt caaggcgtaa aggtcgaagg acggagtgtc ggacccggca
     6781 cagtcgccgg gggtcagagc gccactgacg ctctggccga gcgcgtatgc tgcaatccgg
     6841 cctgaagcga tgaacgtggc gggcgacacg cccgtcaccg ttgcactgac cgcgaccggg
     6901 ctcagcgcga taccaagcgt gagttggatc cgggacctgc ccgcggcatc cgtcgtgtcg
     6961 agcgcccgcg agagcgtcgc ggcgccgcct gtcacctgcc aggccaccgg cgcaccggcg
     7021 aacggcttgt tgtcgctgcc ggtgagggtg acctcgaggg agtcggggag ggtgtcgacg
     7081 agacagcctg attgctggtt gccgcccgtg acggcaaggg tccgcggcgt gaccggcccc
     7141 cccgaatcgc cgccacaacc agacagcgcc cagagtgcgg tcacagtggc gagacggccg
     7201 aagtggcgtg ggaacaggtt catggcgacc tcccaatacg gctagggatg tcacgccctg
     7261 ttgccggcag agcgggacaa cgcccgttac gcgaaggacg ttccctgggg gcgcacgcta
     7321 atctcggcgg gcctggtcaa ttggtggaca attcctgagc tctgcctctg gtcgcggtta
     7381 cgccagaccc ctatgcttgg aatcgtcccc gccatccgag gcgctgcacg ccagaggtgt
     7441 caccgtccct acaagccgcc ctcgcgggcc gctacgccat cgagcgcgag ctcggtcacg
     7501 gtgggatggc cacggtctat ctcgcgcggg acttgaagta cgctcgaccg gtcgccctca
     7561 cggtgctgcg gcccgagctc accgccgcgc tcggcgccga gcggttcttg cgcgaagtcc
     7621 gcatcagcgc ccggctcgac catcctcata tcctcacgct catcgattcc ggcgagtccg
     7681 acggcttcct ctggtacgtg ctgcccttcg tccacggcga gtccttgcgg gaggcgctga
     7741 agcgcgagcg gcagctcgag ctcgaccggg ccctggccat cacgcgccag atcgccggcg
     7801 ccctcgacta cgcccctcgg cagggcgtga tccaccgcga catcaagccc gagaacatcc
     7861 tgctgcacga gggcgaggcg atgctggcgg acttcggcat cgcgatggcg gtgcgggagg
     7921 ccgggggaag ccggctgacc gagtcggggc agtcgctggg gacgccgcag tacatgagcc
     7981 aggagcaggc gacgggcgag cgggagctcg acgcgcgcag tgacgtgtac tcgctcggcg
     8041 cggtgctgta cgagatgctg acgggcgaag cgccgcacac gggcgcgacg atgcaggcgg
     8101 tgatcgcgaa gctcatgacc gagcgaccga cgcgcgtgcg cacggtgcgg gacacagtcc
     8161 ccgagggtgt ggacaacgcg gtagcgaagg cgctggccaa agtcccggcg gaccggtttc
     8221 acggggccgc cgagttcgcc gaggcccttg acgtgcgcgg cgcggtcgcg cgagggactc
     8281 tgcctcgatc gctcaaatgg gcgctcggaa cggcggggct tgtcaccgtc gccgctgtcc
     8341 tcgtgatcct ctctcgtctc cggcaggaga gcatcgcgct tggcaatgtc tcgcagatga
     8401 ccttcgagcc ggggctcgag atcgagccgg cgatctctcc tgacgggaag ctggtggcgt
     8461 acgctggtgg tccgctgtcc cagacccgga tctatgtgcg ccagccggga ggtcgccccg
     8521 tggcgctcac ggcagactcc ggtccgccac agcggcgccc gctgtggtca ccgaacggca
     8581 cgcgcatcct gttcgagaca gacgaggacc tgttcctcgt ccccttgttt ggtggcgtgc
     8641 ctcgtctgat ggcgcgccgt gcatgctgcg cggcatggtc cccggacggt cgggaagtgg
     8701 cctatgtggg gtcaccccgg cgcccgggca gctttgaacg gcccgagtcg ctcttcgtgc
     8761 ggccgcccga cggcggggcc tcacgcttcg tcaacgtcgc tttcgacccc cattccctcg
     8821 cctggtcacc cgatgggaag tggcttgcgc tcgtctcggg aaacgatcaa ttcgcggtgg
     8881 gcacggcgtt gttcggaaac acggggccga gcgccatcgt cctcgttccg gccgccggcg
     8941 gtgcgcaagt gccggtcacc gacaacacca agctgaacgt gagcccggta tggacgccgg
     9001 acggtcacca ccttctcttc gtgtccgacc gcgagggcgc ccgcgacgtg tacgcggtgc
     9061 gccttggcag ctcgggcgcg ccggccggcc ctccctcgcg catcacgacc ggagtgaacg
     9121 cgcactcgat cagtctttcc gcctcggcgg ggcggctcgc gtactcggtc tacacctcgc
     9181 gcgcgaacgt ctggacaatc gcgatcccca ccgacgggcc ggtctcagtg gacggtgcca
     9241 tccagatcac cacaggcaac cagaccgtgg agcagatggc ggtctctccg gacggtcggt
     9301 ggctctactt cgactccaac cgcttcggga acgcggacat ctaccggatg cccgtggacg
     9361 ggggggagcc ggagcagctc accaccgacc ctgccgacga cttcggtccg gacgtgtccc
     9421 ccgacggccg ctggatggcg ttctactcca tgcacctcgg gacccgcgac atcttcgtga
     9481 tgccggcgga gggcggggag atggagcggg tcacggacga ccccggggag gagcggattc
     9541 cgagctggtc gccggacggg cggtcgctgt cctacttcct caacggaacc ggcgcgcgtg
     9601 acggtttgta cgtgatttcg cgggatgcga gcgggcgctg ggggcgcccc cggcaagtct
     9661 ggaatcaccc ggatgtgggc cgttggtctc cagacgggca aaccctggtc agcacctgga
     9721 ctgacggcgt ctggttgatc cccgtcgcgg gaggccggcc tcagcgggca agtccgttcc
     9781 cgaccctgac gatcccggag caggtgtggg cgtactggtc gcgtgatggc cggacgatct
     9841 atttcaaggc acgcgaccgc gcgggccggt cgtcgatctg gggagtcgcc gcggcgggcg
     9901 ggcaccccca cacgctggtg cggttcgacg acccggcaag gccctcctac tacacgtcat
     9961 gggcaagtga cggccggcgg ttctacttca ccatcaacga ccggcagagc gacatctgga
    10021 tcgccgagtt gaatggcttg aagtgaacag gcggagcacc ggccgttaat cgaatcgact
    10081 acctggcgga cgacctgagg aggtgaacaa cgtacaaggc acctatcccc aacgacagaa
    10141 agctgaatcc ggccactttg accaggaatt cgaatcggct tttcatgttg acagccctcc
    10201 ccggtagacg ggatgtgtca acctgaatcg agaaagcagc gctgacagag cgagttatac
    10261 acgctagtcc cgcgcccgct cccgcagaaa cgtctcgatc ccctcggcca ccgcgcgcgc
    10321 gtagcgccac tgcccgtcct cgctgcgcag catggcttcc tgctcgggga gcatgatgaa
    10381 gagcccctcg gtcagcgccg ccggcatcca cgtgggccgc accagggcgt aatcgccccg
    10441 ccccatcccc aggtcgcgag tccccagcgc gcgcaccagc gcgcgatcga gcgcgcgcgc
    10501 caggtcggcg ctgcgcggct ggaagtagta gacactcgtg ccgttgttga cgaacggatt
    10561 cacgccgtcg gggagcgcgt tggcgtggat cgagaccagc acgtccgcgt tgcgctgctc
    10621 ggcgaaccgg gtgcgcggat agagatccac cggagtcatg tcgctgcgcg tgagcagcac
    10681 cgtcgcaccc gcacgcgcga gcagccgctc cgccttctgc gctacggcga gcaccgccac
    10741 cggctcccac aggcgcgtcg ggcccgtggc accgacgggc ggatggcccg ggtccagcac
    10801 gatggtccgc ccccgcaacg ggtggcgcgc atcgatggcg gggggccgcc gcacgtcgag
    10861 cagcagatcc cggccgtcga agcggctgcg gtagccccac acgcgccgcg acagctcgag
    10921 cgtgatcgtc agctcgtcca ccgattcctg gcggtagctc atgcgggtga ccagtggatc
    10981 ggtgccgccg tactgcatcc agttcacgtc ggaggcgccg ccgtagatcc gcagcgtcag
    11041 cgaccggtcc ccctcctcca cccggtaggg caaccgggcc ggaagcggca cgcgcagcgt
    11101 cacccatccc gattccggat gcagacgcac cgagcccacg gtgccggccg gtggcggcgt
    11161 gcccgccggc agctcgtgca cgtcggcggc gttcacccag gccgcggcac tgcgcgacag
    11221 agcgagacgc acctcgccgt tcaaccaccc cgcgaccgcg gccgtcgtcc cggtcgggaa
    11281 gaaccactgg taggtgcccc cgggtaccgc cttcccgacg gtgagcgaat cggtggtgcc
    11341 gctatgcgcc gtgtcgtcgt ccagcacc
//
LOCUS       DBNGZW010000440         4877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1335823, whole genome shotgun sequence.
ACCESSION   DBNGZW010000440 DBNGZW010000000
VERSION     DBNGZW010000440.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4877
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1335823"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..636
                     /locus_tag="AC16JC_10440"
     CDS             <1..636
                     /locus_tag="AC16JC_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165153.1"
                     /translation="RDWLGGPVTPWLDLKPLLGRVYVQLVTAPSQAVRRCFAGALPAC
                     RDALSLTDTANQITRWYGPEERRYLVATQLAGFQSSGFLARQGREAEFHSCAAGSDAA
                     CLNLLIAFSPGATPAPLDYGARYTLLATAVTLGGQEAFSRLLASSKEPIGRRLAEASL
                     VTEDSLVARWRADVLAARPKPVSLPPWGPLIAILWAGVFTGCALRSSRWRV"
     gene            624..1751
                     /locus_tag="AC16JC_10445"
     CDS             624..1751
                     /locus_tag="AC16JC_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165154.1"
                     /translation="MARVRWWIGSALLVCATVGFVYLPPRAAPLFARRYREEPLPPYR
                     YRVQALGDAWRSAALELRLLQYREELRPELERRRALDISGPAVMLDWPDVPDSVRRRV
                     AKYLDNDWKELRLGVSKISVGIVIAVNRKADPTIPGEPSVQTVYLLPDSTDRSTCIVY
                     QTFTSAWANWATSTDASKKFLANLGPCAFYAAFGAPGREIERWLSARDFDLAIAPLWT
                     RPAQVEGWPNASFIRNNARFWFPPDGVACLAGRAARCRSAALEAPPTSRDASRFVTLN
                     QRWRPPALAGGTHYLADVIRTVGRERFQRFWNSELPPDTALAEALRMPVGEWTRRWQA
                     DLVPPIQLGAAPTAGSALLGVLLALAAVGLVLRTVAHREVR"
     gene            1748..2203
                     /locus_tag="AC16JC_10450"
     CDS             1748..2203
                     /locus_tag="AC16JC_10450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165155.1"
                     /translation="MSPRRLATCTVAWLLAPVAAVGQGRWERQVRAHLAQASATLTTS
                     AYVVTPGGRTGMLNNSETAADTVTLAAGFTYVLVGVCDDDCRGLELGLFAANGYEVDA
                     ARRAGNAPIVRVTPRETQRFRFTIRMTGCGMNPCWYGVAVYRRRLSDGD"
     gene            2265..3581
                     /locus_tag="AC16JC_10455"
     CDS             2265..3581
                     /locus_tag="AC16JC_10455"
                     /inference="COORDINATES: protein motif:HMM:NF016241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HIR5165156.1"
                     /translation="MGRLLFVVALLACGAGAAGAQAPGDSIRSDSTLRVFFDCPGFAA
                     GCDFDFLRTEITWVDWVRNREDADVHALVTTQPTGGGGDAYTLTVIGLRGFTGKADTL
                     HYFAAATITPAEARGGFAHILELGLARYAAARPQAGRLSVVYASSAESHPSATRDRWN
                     YWVFTISAGTDINGQSQTRSTSYNGSLEANRTTASWKLNFGLNEYYNEDVFKDVPVYD
                     TLGNQIGTQDIKSVSRQYGGDALAVRSLGPHWSMGLQGSISRSTFSNLDLSLSIGPAI
                     EYDIFPYAQSTRRQLTLQYGLTSRYFNYADTTIFERTTETLGQQSLTMSLGVTQLWGS
                     AHVSLTGSHYLQDLHKNRLTLIGSVNLRLVKGLSLNVFGNVARIHDQLSLAKAGASDA
                     EILLQRRELLTSYRYFTYVSLRYHFGSTLNNVVNPRFGGAGTFFCC"
     gene            3646..4728
                     /gene="ada"
                     /locus_tag="AC16JC_10460"
     CDS             3646..4728
                     /gene="ada"
                     /locus_tag="AC16JC_10460"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011750468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HIR5165157.1"
                     /translation="MTHAAAVPTADATRQWRIVLARDRRYDGAFVYAVRSTGIFCRPS
                     CPSRRPRRTLVQFYPIPEAAEAAGFRACRRCRPTQVPAADPAVAVVRELCRAIDAHPD
                     EPTDLTTLGRRAGLSPHQVLRAFRRVLGVSPKAYGDARRLDRLKAALKQHRHVSPAIY
                     DAGYSSSSRVYERAGAALGMTPSAYRRGGRGAEIHYTVATSPLGVLLVAATARGVCRI
                     SLGDTAAGLERDLRAEFPASTIRRDPGRLAVWVQRILRHVEGRAPHLDLPLDIRATAF
                     QQRVWAELRKIPYGSTRSYHAVARAIGRPRSSRAVSHACATNPVALAIPCHRVVREDG
                     ALGGYRWGIERKRTLLERERKGAAAG"
BASE COUNT          720 a         1749 c         1581 g          827 t
ORIGIN      
        1 cgcgactggc tcggcggtcc cgtcacgccg tggctcgacc tcaagccgct gctcggtcgg
       61 gtgtacgtgc agctcgtcac ggccccctcc caggccgtgc gtcgctgttt cgcgggcgcc
      121 ctgccggcgt gccgtgatgc gctgtccttg accgacaccg ccaaccagat cacccgttgg
      181 tacggccccg aagagcgacg ctatctcgtg gccacgcagt tggccgggtt ccaatcgtcc
      241 ggttttctcg cacgacaggg tcgtgaagcg gaatttcact cctgcgccgc cggcagtgat
      301 gcagcgtgtc tcaacctgct cattgccttc tcgcccggcg ccaccccggc accgctggac
      361 tacggagcgc gctacacgct gctggcgacg gccgtcaccc tgggcggtca ggaggcgttc
      421 tcccggctcc tcgcctcctc gaaggaaccg atcggcagac gcctggccga ggcctccctc
      481 gtcaccgaag actcactcgt cgcgcgctgg cgcgcggacg tcctggcggc gcgtcccaaa
      541 ccggtatcgc tgccgccctg ggggccgttg atcgcgattc tgtgggccgg cgtgttcacg
      601 ggttgtgccc tgaggagctc gcgatggcgc gtgtgaggtg gtggatcgga tcggcgctgc
      661 tcgtctgcgc gaccgtaggc ttcgtgtatt tgccgccgcg tgccgcgccc cttttcgcca
      721 ggcgctacag ggaggagccc ctcccgccat atcggtatcg ggtgcaggcg ctgggcgacg
      781 cctggcggtc cgcggcgctc gagctacgcc tgctgcagta ccgcgaggaa ttgcgaccgg
      841 agctggagcg ccgccgagcg ctcgacatct ctggccccgc cgtgatgctg gattggccgg
      901 acgtgcccga ttcggtgcgc cgccgcgtcg cgaagtatct cgacaacgac tggaaggaac
      961 tgcggctcgg agtctcgaag atctcggtag gcatcgtgat cgcggtcaac cggaaggcgg
     1021 acccgacaat tccgggcgaa ccttcggtcc agacggtcta tctgttgccc gactccacgg
     1081 atcgcagcac gtgcatcgtc taccagacct ttaccagcgc ttgggcgaac tgggcgacgt
     1141 ccacggacgc ttccaagaag tttctcgcca acctggggcc gtgtgcattc tacgccgcat
     1201 tcggcgctcc gggacgggaa atcgagcgat ggctgagtgc ccgagatttc gatctggcga
     1261 tcgcgccctt gtggacgaga ccggcgcagg ttgagggctg gcccaacgcc tccttcatcc
     1321 gcaataacgc acggttctgg tttccgcccg acggggttgc ctgcctcgcc gggcgggcag
     1381 cgcgttgccg atccgccgcc cttgaagcac caccgacgag ccgcgacgcc tcgcgtttcg
     1441 tgaccttgaa tcagcggtgg cgaccacccg ccctggccgg tgggacgcac tacctcgccg
     1501 acgtcatacg aaccgttggc cgcgagagat ttcagcgatt ctggaattcc gagcttcccc
     1561 cggacacggc gctcgctgaa gcgctgcgca tgccggtcgg tgagtggacg aggcggtggc
     1621 aggccgatct cgttcccccg attcagttgg gtgcggcgcc gacggctggt tcggcattgc
     1681 tcggcgtgct cctcgccctg gcagccgtcg gcctcgtgct gcggactgtg gcgcatcgcg
     1741 aggtccgatg agcccgcgcc gcctggccac ctgcacggtc gcctggctgc tcgctcccgt
     1801 cgccgcggtg ggccagggac gctgggagcg gcaggtgcgg gcccacctcg cgcaggcgag
     1861 cgccacactg accacttccg cgtacgtggt cacgcccggc ggccggaccg gcatgctgaa
     1921 caacagcgag acggccgcgg acaccgtgac cctcgctgcc gggttcacct acgtcctggt
     1981 cggcgtgtgc gacgacgact gccgcgggct cgagctcgga ctgttcgccg ccaacggcta
     2041 cgaggtggac gccgcccgtc gcgccggcaa cgcgccgatc gtccgggtca cgccccgcga
     2101 gacccagcga ttccggttca cgatccgcat gaccgggtgc ggcatgaacc cctgctggta
     2161 cggcgtcgcg gtctaccgcc gccggctgtc ggacggagac tgacggcgaa ccccgttcgc
     2221 acgctataga atcgacctca tcacttctcc cgcggagaac caggatgggc cgtctactct
     2281 tcgtcgtcgc cctcctcgcc tgcggagcgg gggcggccgg cgcgcaggct ccgggtgatt
     2341 ccatccgaag cgattcgacg ctccgcgtgt tcttcgactg tcccgggttc gccgccggat
     2401 gcgatttcga cttcctccgc accgagatta cctgggtcga ttgggtccgc aaccgggagg
     2461 acgccgacgt gcacgcgctc gtcacgacgc agccgacggg cggcggcggc gacgcgtata
     2521 cgttgacggt gatcggattg cgcggcttca ccgggaaggc cgataccctg cactactttg
     2581 cggccgcgac gatcaccccg gctgaagcgc gtggcggctt tgcccacatc ctggagctcg
     2641 gattggcgcg atacgcggcc gcaaggccgc aggccggccg gctgtcggtc gtctacgcat
     2701 cgtccgcgga gagccacccc tcggcgacgc gcgaccggtg gaactactgg gtctttacca
     2761 tctccgccgg caccgatatc aacggacagt cgcaaacacg ctccacgtcc tataacggct
     2821 ccctggaggc caatcgcacg accgcgtcct ggaaactgaa ttttggcctc aacgagtact
     2881 acaacgaaga cgtcttcaag gacgtgccgg tctacgacac gctgggcaat cagatcggca
     2941 cgcaggacat caagagcgtt tcgcgacagt acggcggaga tgcgctggcg gtgcggagtc
     3001 tgggccccca ctggtcgatg ggcctgcagg gatcgatttc ccgctccacg ttcagcaatc
     3061 tcgacctctc gctcagcatc ggcccggcga tcgagtacga catctttccc tacgcccaat
     3121 cgacccgccg tcagctgacc ctgcagtacg gcctcacgtc gcgttatttc aactatgccg
     3181 ataccacaat cttcgagcgc accacggaga ccctcggaca gcagagcctc accatgtcgc
     3241 tcggggtcac ccagctctgg ggctccgccc acgtgtcgct cacgggctcc cactatctgc
     3301 aggacctcca caagaaccgg ttgacgctca tcggcagcgt gaacctccgc ctcgtgaagg
     3361 ggctctccct caacgtcttc ggcaacgtgg cccgcattca cgatcaactc tcgctcgcca
     3421 aagccggcgc atcggacgcc gagatcctgc tgcaacgccg cgagctgctc acatcctacc
     3481 ggtacttcac gtacgtcagt ctgcggtacc atttcgggtc caccctgaac aacgtcgtca
     3541 acccgcgatt cggcggcgcg gggacgttct tctgttgttg agaggcggga ctttgcgcgc
     3601 aaggagcgga tagatcgcga acgggaccgg cgctatgttg agccgatgac ccacgccgcc
     3661 gccgttccga ccgccgacgc cacacgccag tggcgcatcg tcctggcccg ggatcgccgc
     3721 tacgacggcg ccttcgtcta tgcggtgcgc tcgacgggga tcttctgccg cccctcctgc
     3781 ccgagccggc gtccgcgccg cactctggtg cagttctatc ccatcccgga agcggcggag
     3841 gcggcgggct tccgggcgtg ccgccgctgc cgcccgacac aggtgcctgc cgccgatccg
     3901 gcagtggccg tcgtgcgcga gctgtgccgc gccatcgacg ctcaccccga cgagccgacc
     3961 gatctcacga ccctcggccg gcgcgccggg ctctcgcccc atcaggtgct gcgcgcgttc
     4021 cgtcgcgttc tgggcgtcag tcccaaggcc tatggcgatg cccggcgcct cgaccggctc
     4081 aaagccgcgc tcaagcagca tcgccacgtg agcccggcga tctacgatgc cggatacagc
     4141 tcctcgagcc gcgtgtacga gcgcgccggc gcggcattgg gcatgacgcc gtccgcctat
     4201 cgccggggtg ggcgcggcgc ggagatccac tacaccgtcg ccacgtcgcc gctgggcgtg
     4261 ctgctcgtcg ccgccactgc gcgcggcgtg tgtcgcatca gcctgggaga caccgcggcg
     4321 gggctcgagc gcgacctgcg tgccgagttc cccgcctcga caattcgccg cgaccccggc
     4381 agactcgccg tctgggtgca gcgcatcctg cgacacgtcg aggggcgggc cccgcacctc
     4441 gacctgccgc tcgacatccg cgccacggcg ttccagcagc gcgtctgggc cgagctgcgc
     4501 aagatcccct acgggagcac gcgatcgtac cacgccgtgg cccgcgcgat cggacgtccc
     4561 cgctccagcc gggcggtgtc tcatgcctgc gccacgaacc cggtggcgct cgccattccc
     4621 tgccaccgcg tggtgcgcga agacggcgcg ctgggcggat atcgctgggg tatcgagcgc
     4681 aagcggacgt tgctcgagcg cgagcgcaag ggcgccgctg caggctgaag gagctttcac
     4741 cggcttttca cccgggcgca tcccccggtg ccgcatgatg cgctcatggg cgagaccggt
     4801 ttcgaagtgc agctcacgct gcagcgcgag tatcagatca cggccgattt cggcttcccg
     4861 gaggcacccg agctcac
//
LOCUS       DBNGZW010000441         1396 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1052186, whole genome shotgun sequence.
ACCESSION   DBNGZW010000441 DBNGZW010000000
VERSION     DBNGZW010000441.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1396)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1052186"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="AC16JC_10465"
     CDS             <1..779
                     /locus_tag="AC16JC_10465"
                     /inference="COORDINATES: protein motif:HMM:NF019352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR5165158.1"
                     /translation="IRHTRLTIAPGVTYNAWTFDGRVPGPALRVTVGDTVDFTLVNRA
                     PMPHSMDFHAAEIAPNRAYVNVLPGESLHYRFVARVPGAFLYHCGTAPVAMHIANGMY
                     GAIIVDPKQPRAPASEFVLVQSEFYTQKAGGADTAVLALDWQKLLGQAPDYVTFNGRS
                     AQYATAPILVPPRQLVRLYVVNAGPNRVSSFHVVGGIFDHVYLDAATAPTLAGVQTVG
                     VPVGGGAVFEVRLAEPGDYPFVSHAFADATKGAVGVLRAK"
     gene            865..>1396
                     /locus_tag="AC16JC_10470"
     CDS             865..>1396
                     /locus_tag="AC16JC_10470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165159.1"
                     /translation="MADRRLTPVPDVPRHQRRWTPPTLSASSAMALRAPLRGAIYVVL
                     IGLTAAGLIYSLQGVIARNQGVQVRLAEMEGWVNLLNAREWEARTGVISSDHQTDVAE
                     SQQHLRSIITELRERTGNPPSQQSGSRSAITNLQQVEADVHVYLVAVDSMFQLLSRDK
                     RPQAQTLAEQTVDPAFE"
BASE COUNT          222 a          517 c          449 g          208 t
ORIGIN      
        1 agatccggca caccaggctg accatcgcgc ccggcgtcac ctacaacgca tggacgttcg
       61 acggcagggt gcccggcccc gcattgcgcg tcacggtcgg cgacaccgtc gatttcacgc
      121 tcgtcaaccg cgccccgatg ccgcacagca tggactttca tgcggccgag atcgcgccca
      181 accgcgccta cgtcaacgtg ctgccgggcg aatccctcca ttaccggttc gtggcccggg
      241 tccccggcgc cttcctctat cactgcggta ccgcacccgt cgcgatgcac atcgccaacg
      301 gcatgtacgg cgcgatcatc gtagacccga aacagccgcg cgcgccggcc agcgaattcg
      361 tcctcgtgca gagcgagttc tatacgcaga aggcgggcgg cgccgatacc gcggtcctcg
      421 cgctcgactg gcagaaactg ctcgggcagg cgcccgacta cgtcaccttc aacggccgct
      481 cggcgcagta cgcgaccgcg ccgatcctcg tcccgccgcg gcaactggtc cggctgtacg
      541 ttgtgaacgc cggcccgaac cgcgtgtcgt cgttccacgt ggtcgggggg atcttcgacc
      601 acgtgtacct cgacgccgct acggcgccga ccctcgccgg cgttcagacg gtcggcgtgc
      661 cggtgggcgg cggcgccgtg ttcgaggtac gcctcgccga gcccggcgac tacccgttcg
      721 tctcccatgc gttcgccgat gccacgaagg gcgccgtggg ggtcctgcgc gccaagtgat
      781 gattgcggcg ccgtcgccac aggcagggat cgccacacgc ccgtagccga acgcaaacga
      841 ccgagcgatc attgagacac ctgcatggcc gaccgccgac tcaccccggt tcctgatgtc
      901 ccgcgacacc agcggcgctg gacgcctccg acgctcagcg ccagctccgc catggcgctg
      961 cgggcgccgc tgcgcggcgc gatctacgtc gtgctcatcg gcctcaccgc ggccggtctc
     1021 atctactcgc tccagggcgt gatcgcccgc aaccagggcg tccaggtccg tctcgcggag
     1081 atggagggct gggtcaacct gctgaacgcg cgggagtggg aggcgcgtac gggagtgatc
     1141 agctccgatc accagaccga cgtcgcggag agccagcagc acctgcgttc catcatcacg
     1201 gagctccgtg agcggaccgg gaacccgccg agccagcaga gcggctcccg ttccgcgatc
     1261 acgaacctcc agcaggtgga ggccgacgtg cacgtgtacc tggtcgccgt cgactcgatg
     1321 ttccagctgc tgtcccgcga caagcggccc caggcgcaga cgctggcgga gcagaccgtg
     1381 gaccccgcgt tcgaga
//
LOCUS       DBNGZW010000442         5406 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1202374, whole genome shotgun sequence.
ACCESSION   DBNGZW010000442 DBNGZW010000000
VERSION     DBNGZW010000442.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5406)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1202374"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..332)
                     /locus_tag="AC16JC_10475"
     CDS             complement(<1..332)
                     /locus_tag="AC16JC_10475"
                     /inference="COORDINATES: protein motif:HMM:NF018899.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HIR5165160.1"
                     /translation="MIRRLLSSVAVLAVLVVPSRASAQDQGPGGRPDSVLVAGQRRVG
                     TAVILQTAGLVPGRFVTYRDIQRAIRALYATGQFDDVRVDQDTTGDMQRLTIQVHERP
                     ILQHWTVRG"
     gene            complement(329..2809)
                     /locus_tag="AC16JC_10480"
     CDS             complement(329..2809)
                     /locus_tag="AC16JC_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HIR5165161.1"
                     /translation="MNGYNFTDRVRKVLQMAREEAARLHHEYVGTEHILLGLIREGEG
                     VAAAVLTNLNVDLEEIQQKIEETVKKGKAAAAAGPDLPYTSRAKKVLELAMSEARELN
                     HSYVGTEHLLLGLLREEKGIAAQVLTDAGVNLEQARAETLRLLGSEMPSAAPSGGAST
                     PAPSPKSEKKSKTPALDHFCRDLTQLAADGALDPTIGRQKEIERVMEILSRRKKNNPV
                     LIGEPGVGKTAIVEGLAQLIANGQCPDALKDHRVLSLDMAAVIAGTKYRGQFEERLKA
                     VINEIAQNRSIILFIDELHTLVGAGAAEGAVDASNMLKPALARGELQCVGASTLNEYR
                     KYIEKDGALERRFQTVIVDPPSVDETIEILKGLRKRYEDHHRVVIPDLTLDAAAKLSE
                     RYITDRFLPDKAIDVIDEAGARARLATQVPPPEVVERKEKLEQISRDKENAVRDQNFE
                     RAAALRDSERELQNEIRRKQEEWERERQTRRPTIGEEQVAFIVSRWTGIPVTRLQEAE
                     TARLLRMEEELHQSVVGQDEAITVISRAIRRSRAGLKDPKRPIGSFVFSGPTGVGKTE
                     LARALARFLFADATALIRVDMSEYMEKFSVSRLIGAPPGYVGYEDSGALTKAVRRKPY
                     SVVLLDEIEKAHPDVFNILLQVLDEGHLTDNYGRVIDFKNTVVIMTSNVGARDMLKGR
                     TLGFAQGDTKGDFERMAEKVKEEIGKVFNPEFLNRLDDVIVFHPLTRDHIAQIVTIIL
                     RDVQKRLGEEELTLRLTPAANDFLVDHGYDEHYGARPLKRAVQKYVEDPLSEKILVGE
                     FARGDEIEVDVAPDKERLAFRALSGSKA"
     gene            complement(2823..3899)
                     /locus_tag="AC16JC_10485"
     CDS             complement(2823..3899)
                     /locus_tag="AC16JC_10485"
                     /inference="COORDINATES: protein motif:HMM:NF012443.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--guanido phosphotransferase"
                     /protein_id="HIR5165162.1"
                     /translation="MIDLTLLPDGGLGWLHASGPHPQIVLSTRIRLARNVIGHPFSQR
                     ATDAERRAVLARVTDAAGASERLRGAVLLRVDELSRPDRQLLHERHLVSKELAALDGE
                     GARPRVGAAVLVDDPGGVMINEEDHLRMQAMLSGFDLDAAYAVLESIDSELGRLLPFA
                     FHSEFGYLTSCPTNAGTGLRASVLIHLPGLVLTKEIGKVLQGLTQVGLTFRGLYGEGS
                     EVVGNFFQLSNQTTLGKAEGELLDHLGRIVRQVIEYEEQARDVLLKTAAGTVEDKVWR
                     AYGLLRYARSLSFEETMNLLSGVRLGVGLNLITGLSVYTLNKLLIHTQPAHLAAMEGR
                     QVGDPELPVIRATYVRSLLGAETT"
     gene            complement(3896..4387)
                     /locus_tag="AC16JC_10490"
     CDS             complement(3896..4387)
                     /locus_tag="AC16JC_10490"
                     /inference="COORDINATES: protein motif:HMM:NF014232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HIR5165163.1"
                     /translation="MRCDNCKERDAVINLTQVEHDTKVTLHLCEQCAQEKGIETGTTA
                     LKSTLGTFLSAMGKGTNLAVPADTVRCPSCGATLNDFRDSGRLGCAQCYQVFAEQLRD
                     LLRRLHGSSQHVGERYIAPGADGETSAASELLELRDQLRRAVESENFERAAELRDQIR
                     VRE"
     gene            complement(4387..5088)
                     /locus_tag="AC16JC_10495"
     CDS             complement(4387..5088)
                     /locus_tag="AC16JC_10495"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5165164.1"
                     /translation="MTALLEARGIRKVFAGGDGRPLEVLASVDLDVRRGELIAVVGAS
                     GAGKSTLLHLLGALDRPTAGDVWLEGSRYADLGPQGLAEMRNRKLGFVFQFHHLLREF
                     TALENVMMPLLIGGVAEREARSRAEERLSAVGLAGRMTHRPAELSGGEQQRCAVARAL
                     VHDPSLVLADEPSGNLDHASSEHLHEMLFRLAREFETAIVIVTHNRQLAARADRILWL
                     EDGRLVAVGSVDAIP"
     gene            complement(5085..>5406)
                     /locus_tag="AC16JC_10500"
     CDS             complement(5085..>5406)
                     /locus_tag="AC16JC_10500"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR5165165.1"
                     /translation="EIGILMAMGLRRAAVRRVFLVQGVLIGLVGTLVGATLGIGVGAM
                     INRGEWIPLDPSIYFIDHLPVHTQLLDVLVVVAASLVIATLAPLYPALLAARLDPVPA
                     IRSE"
BASE COUNT          808 a         1962 c         1716 g          920 t
ORIGIN      
        1 ccgcgcaccg tccagtgctg caggatgggc cgctcgtgca cctggatcgt gaggcgctgc
       61 atgtcgccgg tggtatcctg atcgacccgg acgtcgtcga actgcccggt cgcgtagagc
      121 gcccggatcg cccgctggat gtcgcgatag gtgacgaagc ggccgggcac gagcccggcg
      181 gtctgcagga tgacggccgt gccgacccgc cgctggccgg ccacgagcac cgaatccggg
      241 cggccgccgg ggccctggtc ctgggcggag gcccgggacg gcaccacgag gaccgcgagg
      301 acggcgaccg acgaaaggag ccggcgaatc acgccttcga gccggagagc gcccggaacg
      361 ccaggcgctc cttgtcaggg gccacgtcga cctcgatctc gtcgccccgc gcgaactcgc
      421 cgaccaggat cttctccgac agcgggtcct cgacgtattt ctgcaccgcg cgcttcaacg
      481 gccgggcgcc gtagtgctcg tcgtagccgt gatccacgag gaagtcgttc gcggccggcg
      541 tgagccggag cgtgagttcc tcctcgccca gccgcttctg cacatcgcgc aggatgatgg
      601 tcacgatctg cgcgatgtga tcgcgcgtca gcggatggaa cacgatcacg tcgtccaggc
      661 ggttgaggaa ctcggggttg aataccttgc cgatttcctc tttcaccttc tccgccatgc
      721 gctcgaagtc gcccttggtg tctccctggg cgaatcccag cgtccggccc ttgagcatgt
      781 cgcgggcgcc gacgttcgac gtcatgatca cgaccgtgtt cttgaagtcg atcacgcgac
      841 cgtagttgtc cgtcagatgc ccctcatcca gcacctgcag caggatgttg aagacatccg
      901 ggtgcgcctt ctcgatctcg tccagcagca ccaccgagta cggtttccgg cgcaccgcct
      961 tggtgagcgc gcccgaatcc tcgtacccca cgtaccccgg aggcgcaccg atgagtcggc
     1021 tcacggagaa cttctccatg tattccgaca tgtccacgcg gatcagcgcg gtggcgtcgg
     1081 cgaacaggaa gcgggccagc gcccgcgcca gctcggtctt ccccacgccg gtcggcccgc
     1141 tgaacacgaa ggacccgatg ggccgcttgg gatccttcag gcccgcccgg ctgcgccgga
     1201 tcgcacgcga aatgacggta atggcctcgt cctgcccgac cacgctctgg tgcagctcct
     1261 cctccatccg gagcagccgc gccgtctcgg cctcctgcag ccgagtcacc ggaatccccg
     1321 tccagcgcga gacgatgaag gcgacctgct cctcgccgat ggtcggccgg cgggtctgcc
     1381 gctcgcgctc ccactcctcc tgcttgcggc ggatttcgtt ctgcagctcg cgctccgagt
     1441 cccgcagcgc cgcggcccgc tcgaagttct gatcgcgcac cgcgttttct ttatcccggc
     1501 tgatctgctc cagcttttcc ttgcgctcca ccacctcggg cggcggcacc tgcgtcgcca
     1561 ggcgcgcccg cgccccggcc tcgtcgatca cgtcgatcgc cttgtccggc aggaaacggt
     1621 cggtgatgta gcgctcggag agcttggcgg ccgcgtcgag cgtcaggtcg ggaatcacga
     1681 cccggtgatg gtcctcgtac cgcttccgca ggcctttcag gatctcgatc gtctcatcga
     1741 cgctcggcgg gtccacgatc accgtctgga agcggcgctc gagcgcaccg tccttctcga
     1801 tgtacttgcg gtactcgttg agtgtggagg cgcctacaca ctgcagctcg ccccgtgcca
     1861 gcgcgggctt gagcatgttg gatgcgtcca ccgccccttc ggcggcgccc gcccccacca
     1921 gggtgtgcag ctcgtcgatg aacaggatga tgctgcggtt ctgggcgatt tcgttgatca
     1981 ccgccttgag ccgctcctcg aactgcccgc ggtacttcgt ccccgcgatg acggccgcca
     2041 tgtccagcga gagcacgcgg tgatccttca acgcgtcggg gcattgcccg ttggcgatga
     2101 gctgcgcgag tccctcgacg atcgccgtct tgccgacgcc cggctccccg atcagcaccg
     2161 ggttgttctt cttgcggcgc gacaggatct ccatcacccg ctcgatctct ttttgccggc
     2221 cgatcgtggg atcgagcgcg ccgtcggcgg ccagctgggt gaggtcgcgg cagaaatggt
     2281 ccagcgcggg cgtcttggac ttcttctcgc tcttcgggct cggcgccggc gtgctcgcgc
     2341 cgccgctcgg agcggcggaa ggcatctcgc tcccgagcag acgaagcgtc tcggcgcgcg
     2401 cctgctcaag attcacgccc gcgtcggtca gtacctgagc cgcgatcccc ttctcctcgc
     2461 gcagcaggcc gagcagcaga tgctcggtgc cgacgtagga gtggttcaac tcgcgggcct
     2521 cgctcatcgc gagctcgagc accttcttgg cccgcgaggt gtagggcagg tcggggccgg
     2581 ctgccgccgc ggctttccct ttcttgacgg tctcttcgat cttctgctgg atctcttcga
     2641 gatccacgtt gaggttggtg aggacggccg cggccacccc ctccccctcc cggatcaggc
     2701 cgagcaggat gtgctccgtg ccgacgtact cgtggtgcag ccgggcggct tcctcccgcg
     2761 ccatctgcag gactttccgc actcgatccg tgaagttgta gccgttcatc gccgttccat
     2821 cctcaagtgg tctctgcgcc gagcaggcta cgcacgtagg tcgcccggat gacgggcagc
     2881 tcgggatcgc cgacctgccg cccctccatc gccgccaggt gagcgggttg cgtgtggatg
     2941 agcagcttgt tgagcgtgta tacactgagc ccggtgatca gattcaaccc gacgcccagc
     3001 cgaaccccgc tcaacaggtt catcgtctcc tcgaacgaca gcgagcgcgc gtagcgcaac
     3061 agtccatacg cccgccacac cttgtcctcc accgtccccg ctgccgtctt cagcagcacg
     3121 tcccgtgcct gttcctcgta ctcgatcacc tgccgcacga tgcgccccag gtgatccaac
     3181 aattcccctt cggccttccc cagcgtcgtc tggttggaca actgaaagaa gttgccgacg
     3241 acttcactcc cctccccgta cagcccccga aaggtgaggc cgacctgcgt gagcccctgc
     3301 agcaccttgc cgatttcctt ggttaacacg agaccgggca agtggataag cacgctggcc
     3361 cgaagtccgg tgccggcatt ggtggggcaa gaggtaagat agccaaactc ggaatggaac
     3421 gcgaacggga gcagccgccc cagctcgcta tctatcgact ccagcacggc ataggcggcg
     3481 tccaagtcga atccggacag catcgcctgc atgcgcaggt ggtcttcttc attaatcatg
     3541 actccgcccg ggtcgtccac cagcacggcg gcaccgacgc gcggccgggc cccctcgccg
     3601 tccagggccg ccagctcctt gctgacgagg tggcgctcgt gcagcagttg gcggtcaggc
     3661 cgcgacaact cgtcgacccg gagcagcacc gccccccgca gccgctcgct cgccccggcc
     3721 gcatccgtga cccgcgccag cacggcccgg cgctccgcgt cggtcgcccg ctggctgaac
     3781 ggatgaccga tcacgttgcg cgccagtcgg atccgcgtgg acagcacgat ttgcggatgc
     3841 ggcccggacg cgtggagcca gcccaatccc ccgtcgggga gcagcgtcag atcgatcatt
     3901 cgcgcacccg gatctgatcg cgcaactccg ccgcgcgctc gaagttctcg ctctccacgg
     3961 cgcgacgcag ctggtcgcgc agctccagca gctcgctcgc cgcgctggtc tcgccgtcgg
     4021 cgcccggcgc gatgtagcgc tcgcccacgt gctgactcga gccgtggagc cggcgcagca
     4081 ggtcgcgcag ctgctcggcg aacacctggt agcattgggc gcaacccagg cgtccgctgt
     4141 cgcggaaatc gttcaacgtg gcgccgcagc tcggacagcg caccgtgtcc gcgggcacgg
     4201 ccaggttggt gcccttcccc atcgccgaca ggaaggtgcc gagggtggac ttgagcgcgg
     4261 tggtgccggt ctcgatgccc ttctcctgcg cgcattgctc gcagaggtgg agcgtcacct
     4321 tggtatcgtg ctccacctgg gtcaggttga tgaccgcgtc gcgttccttg cagttgtcgc
     4381 agcgcatcag ggtattgcgt ccaccgatcc caccgccacc agccgtccgt cctccagcca
     4441 gaggatgcgg tcggcccgcg cggcgagctg ccgattgtgt gtgacgatca cgatcgccgt
     4501 ttcgaactcg cgcgcgagcc ggaacagcat ctcgtgcagg tgttcgctcg aggcgtggtc
     4561 caggttcccc gacggctcgt ccgccagcac gagactcgga tcgtgcacca gcgcgcgcgc
     4621 caccgcgcag cgctgctgct cgccccccga cagctcggcc ggccggtgcg tcatccgccc
     4681 cgcgagcccg acggccgaca ggcgctcctc cgcccgcgag cgagcctcgc gctccgctac
     4741 ccccccgatc aagaggggca tcatgacgtt ctccagcgcg gtgaactcgc gcaagagatg
     4801 gtggaactgg aagacgaatc cgagcttgcg gttccgcatc tcggccagtc cctgcggccc
     4861 gaggtcggcg tagcgcgacc cctccagcca gacgtccccc gccgtggggc ggtccagggc
     4921 gcccaacagg tggagcaacg tactcttccc cgcgcccgag gcccccacga ccgcgatcag
     4981 ctccccccgg cgcacgtcga gatcgacgct cgcgaggacc tcgagcggcc gtccgtcccc
     5041 ccccgcgaac accttgcgga tgcctcgggc ctcgagtagc gccgtcattc ggagcggatc
     5101 gcgggcacgg gatcgagacg cgccgccagc agcgccggat acagcggcgc cagggtggcg
     5161 atgacgaggc tcgccgcgac gaccacgagg acgtcgagga gctgcgtgtg gacgggaagg
     5221 tggtcgatga agtagatcga gggatcgagc gggatccact ccccgcggtt gatcatcgcc
     5281 cccacgccga tccccagggt cgcgccgacc agcgtcccca ccagcccgat gagcacgccc
     5341 tgcaccagga agacgcgccg cacggccgcg cgacgcaggc ccatggccat caggatgccg
     5401 atctcc
//
LOCUS       DBNGZW010000443         6903 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_245806, whole genome shotgun sequence.
ACCESSION   DBNGZW010000443 DBNGZW010000000
VERSION     DBNGZW010000443.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6903)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6903)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6903
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_245806"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1271)
                     /locus_tag="AC16JC_10505"
     CDS             complement(<1..1271)
                     /locus_tag="AC16JC_10505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR5165166.1"
                     /translation="MSLAWLVQRVSFRSTGARLAEAALTVSPGALFYRLSVGGQQVGY
                     ASTTVDTLEDSLRLSDVLVIDMPALGRLNRSAGSSVAMLDRSLHLLELRSEVDGVTLR
                     IAARTGAEPGGRVQVTIVAGGDSVHAALPALDSVELPSLWPLRLAFGGGLHRGRTTQV
                     HLFDPFTAALRERTLRVTAESTFVVADSADFDSTAMAWVPLRFDTVRAFRVDGAGDGG
                     GGTSSTWIDVQGRIVRATTPLGFTAERTAFEIAYENFRHRDTLRLLRTTTGVDPDHIV
                     PTTLVTAGQHPDSVEGVAQFRVRLGGGALDGLALDGGRQHLNGDTLTVIRERGAALVA
                     QYRLPDPDPGRRAYLEPDLLISSQDLRVAAQARIVAGADRDPRRVATRLVHWVATAVR
                     RERTDGIGDAAGVLERRRGDANEHTVLFVAMA"
     gene            complement(1313..1825)
                     /locus_tag="AC16JC_10510"
     CDS             complement(1313..1825)
                     /locus_tag="AC16JC_10510"
                     /inference="COORDINATES: protein motif:HMM:NF013798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0072570 - ADP-D-ribose binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HIR5165167.1"
                     /translation="MIRVVVDDLAFLPADALVRPATAQLEPTTPALRRLEQMGGPEFQ
                     RRLEVHTELVVGAAVVTAGGGDLPAEFVIHAVIQSREEPISRRGVARAWRSALERARE
                     WGFARLTVPPLGIGAGNLSIEDAAEILLSELKDLPPAAVPADVAIVVETEDQRVVFES
                     ALRRLHARLS"
     gene            complement(1822..2598)
                     /locus_tag="AC16JC_10515"
     CDS             complement(1822..2598)
                     /locus_tag="AC16JC_10515"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5165168.1"
                     /translation="MIRRYDRQLHETARDRARVAAELLEEARQLTETAMLGLDASGNG
                     DGHGNGNGHDNGNGNGHSNGNGHDTGNGHGNGHGNGNGHAVGSTTQRRTRTLASLRPV
                     GSEAERRLAALDAAVAAQPDSVSLLLERATHLGAMGRFSDARADLERGLLLDPTNMTV
                     RSALGIVLLRKGLWASAVPHLRLVVEHDSWSPTAWFYLGEALNHADDLDGALAAFCRA
                     AELDPHHAKALYGQGVVLDRLRRPEDAARLYRRSREVAAK"
     gene            complement(2595..2915)
                     /gene="cutA"
                     /locus_tag="AC16JC_10520"
     CDS             complement(2595..2915)
                     /gene="cutA"
                     /locus_tag="AC16JC_10520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HIR5165169.1"
                     /translation="MGRQALVAITALGNEADARALVTTLIQDRIIACGTLLGSARSIY
                     RWEGKVREESEVVVLLKTDVAKWDALCAAVERHHPYEVPELLALPVTHGLEPYLLWLA
                     SEVG"
     gene            complement(2967..5258)
                     /locus_tag="AC16JC_10525"
     CDS             complement(2967..5258)
                     /locus_tag="AC16JC_10525"
                     /inference="COORDINATES: protein motif:HMM:NF011071.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming)/trehalose-phosphatase"
                     /protein_id="HIR5165170.1"
                     /translation="MPETTTTLPQVAAPIPVPDLPPRARSLVVVSNRLPFTAERRPDG
                     IQFTRSSGGLVAALDPVLAQRGGVWVGWPGLEQEPDDNPSALVPAAGARVRYRSVSLT
                     GREVAAYYGGFSNRTLWPLFHYLIDRMRSDAHTWAAYEQVNERFARAAAEESTDEDQV
                     WIHDYQLMRVPYYLRRLAPRRRIAFFLHIPFPAYDVYRILPWSRQLLRGLLAADLVGV
                     HVSAYAQHLITCAERLLGCDVDRATDSVHFEGRDVSIQVHPLGIDPGHVETLARAAGP
                     RQPDATEPARVIGLDRLDYTKGILQRMLAVERLLETNPEFQGRFVFTQVLVPSREQVA
                     EYRLLKRELDEAVGRINGRFSDQGWAPIRYLVRSLSPTDLGALYRHADVALVTPLRDG
                     MNLVAKEYVAAQVDEPGVLILSELAGAAEELQEALLVNPFDVDAMAEALHRSLTMPPD
                     ERRARMTALRARVRENSVQAWVVRFLDAAERAAARPRSVSQLDRVRRRLQPWLAARPT
                     VALFFDYDGTLTPIQERPEDAQLSQATRLALESAAHTPNLDTVIVTGRSLADIRRLVG
                     VPGLTYVGNHGFEIEGPGISYRHPDIDRFAGTVSRVAMELERLAVPGAQVEAKGATLS
                     YHVRRVLEAGRDPAARRAAKIMQRRGLRVVSGKLVVEGRPPVPWHKGAAVLFVLVQRH
                     GADWPARVRALYVGDDATDEEAFGSLRGIGRSICVGGPWEGPTLADHALPDPEAVVQL
                     LRWLAAGAFAGGTGLARSRPSGS"
     gene            complement(5407..6780)
                     /locus_tag="AC16JC_10530"
     CDS             complement(5407..6780)
                     /locus_tag="AC16JC_10530"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016877722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR5165171.1"
                     /translation="MDDRHLLQTAAGYAAEYLETLPTRSVAPTHAALDALRAIDRPLP
                     DRPADPLAVLTELHRVASPATTASAGGRYFGFVIGGTLPVTLAANWLASAWDQDANLV
                     VLGPGVAAFEQVAMRWLLEALDLPPDCAAGFVTGATMANFTGLAAARHALLERAGWDV
                     EGDGLFGAPPLTVVVGDEVHISVVKALGLVGLGRNRVVRVPTDAQGRMRAERLPPMDG
                     RTIVCVQVGNVNTGACDPVRDVCRIAHAAGAWVHVDGAFGLWAAAAPARAPLVDGVAE
                     ADSWALDAHKWLNVPYDSGIALVRDAAALRGAMSTPAPYLVHGAAREPCDYTPELSRR
                     ARGIEIWAALASLGRSGLADLVERCCRHATRFAEGLRAAGYEILNDVTLNQVLVSFGD
                     EATTRRVIAALQADGTCWCGGTVWHDRAAMRISISSWATTGEDVERSLGAMIRIAREH
                     RAGVRGT"
BASE COUNT         1029 a         2491 c         2438 g          945 t
ORIGIN      
        1 gccatggcga cgaacagtac ggtgtgctcg ttcgcgtcgc cacgacgacg ctccagtacc
       61 ccggcggcgt ccccgatgcc gtccgtccgc tcccggcgca ccgccgtcgc cacccagtgc
      121 accaggcggg tcgcgacccg gcggggatcg cggtcggcgc cggccacgat gcgcgcctgg
      181 gccgccacgc gcagatcctg actggagatg agcaggtcgg gctcgaggta ggcgcgccgc
      241 ccggggtcgg ggtcggggag acggtactgg gccacgagcg ccgcaccgcg ctcgcggatc
      301 acggtcaacg tgtcaccatt caggtgctgg cgtccaccgt ccagcgccag cccgtcgagc
      361 gctccaccgc ccagtcgcac ccggaactgc gcgacacctt ccaccgaatc gggatgctgg
      421 ccggcggtca ccagggtcgt ggggacgatg tggtcgggat ccactccggt ggtcgttcgc
      481 aggaggcgca gcgtgtcccg gtgccggaag ttctcgtagg cgatctcgaa cgccgtgcgc
      541 tcggcagtga agccgagcgg ggtggtggcc cggacgatgc gcccctggac gtcgatccac
      601 gtggagctcg tgccgccgcc gccatcgccc gcgccgtcga cccggaaggc gcgcaccgta
      661 tcgaagcgga gcgggaccca ggccatcgcc gtgctgtcga agtccgcgct gtcggcgacc
      721 acgaaggtcg attcggcagt gacccgcagg gtgcgctccc gcagcgcggc ggtgaacgga
      781 tcgaacaggt ggacctgtgt ggtacggccg cggtgcaggc cgcctccgaa ggccagtcgc
      841 agcggccaca gtgacgggag ttccacgctg tcgagcgcgg ggagggcggc gtgcaccgaa
      901 tcccccccgg cgacgatcgt gacctgcacc cgcccacccg gctccgcccc ggtgcgggcg
      961 gcgatccgca gcgtcacgcc gtccacctcg ctccgcagct ccaggagatg cagcgaccgg
     1021 tcgagcatgg cgacgctgct cccggcggag cgattcaggc gtcccagggc cggcatgtcg
     1081 atcaccagga cgtcggagag ccgcagcgag tcctccagcg tatccacggt ggtggacgcg
     1141 taccccacct gctggccgcc caccgacaac cggtagaaca gcgcgccggg ggagacggtg
     1201 agcgccgctt cggcgagacg cgctcccgtc gaccggaagg acacgcgctg caccagccag
     1261 gcgagcgaca cggcccagac cgcgagaatc gccgcggccc agtgacgccg ggtcaggaca
     1321 gcctcgcgtg gaggcggcgc agggcgctct cgaagacgac gcgctgatcc tcggtctcga
     1381 ccacgatcgc cacatccgct gggaccgcgg ccgggggcag atccttcagc tccgacagca
     1441 ggatttccgc cgcgtcctcg atcgacaggt tgccggctcc gatccccagc gggggaacgg
     1501 tgaggcgcgc gaatccccac tcgcgcgcgc gctccagcgc cgagcgccac gcccgggcga
     1561 cgccccggcg cgagatcggc tcctcccggc tctggatcac ggcgtggatg acgaactcgg
     1621 cggggaggtc tccgccgccg gcggtgacca ccgccgcgcc gacgacgagc tcggtgtgca
     1681 cctcgagacg gcgctggaat tcgggaccgc ccatctgctc cagccggcgt agcgccggcg
     1741 tggtcggttc gagctgggcg gtggcgggac ggacgagagc gtcggctgga aggaacgcga
     1801 ggtcgtccac gaccacgcgg atcacttcgc ggcgacctcc cgggaacggc ggtacaatcg
     1861 cgcggcgtct tcgggacggc gcagccggtc gagcacgacc ccctggccgt acagcgcctt
     1921 cgcgtgatgc ggatcgagct cggcggcgcg gcagaaggcg gcgagggctc catccaggtc
     1981 gtcggcgtgg ttcagggctt cgcccaggta gaaccacgcg gtcgggctcc agctgtcgtg
     2041 ttcgacgacc agccgcaggt gcggtaccgc actcgcccac agccccttgc gcagcagcac
     2101 gatgccgagc gcgctgcgca cggtcatgtt cgtaggatcg agcagcagtc cgcgctcgag
     2161 atcggcgcgc gcgtccgaaa agcgtcccat cgcgccgaga tgtgtggccc gctcaagcag
     2221 cagcgagacg ctgtcgggct gcgcggcgac agccgcatcg agtgcggcga gccggcgctc
     2281 ggcttccgaa cccaccgggc ggagggaggc cagcgtgcgg gtgcgccgct gtgtggtgga
     2341 accaacggcg tggccgttgc cgtttccgtg gccgttgccg tggccgtttc cggtatcgtg
     2401 gccgttgccg ttactgtggc cgttaccgtt gccgttgtcg tgaccgtttc cattgccgtg
     2461 gccgtcgccg ttgcccgacg catccaggcc cagcatcgcg gtctcggtga gttgccgcgc
     2521 ttcctccaga agctcggcgg ccacacgcgc gcgatcccgc gccgtctcgt gcagttgtcg
     2581 atcgtagcgc cggatcatcc gacctcgctc gcgagccaca acaggtatgg ctccagtccg
     2641 tgcgtcaccg ggagggcgag cagctccgga acttcatagg ggtgatgtcg ctcgacggcc
     2701 gcgcacaatg cgtcccattt cgcgacatcg gtcttcagca gcacgacaac ctccgattcc
     2761 tcgcggactt tgccttccca acggtaaatc gaacgcgcgc tcccgagcag cgtgccgcag
     2821 gcgatgatcc gatcctgaat gagcgtggtc acgagtgcgc gcgcatcggc ttcgttgccg
     2881 agcgccgtga tggcgaccaa cgcctgacgg cccatttgcg gggcagcaaa cgtagcgcgg
     2941 gcgcatcagg ggggcaaggc cgtgagtcac gatccggagg gtcggctgcg ggctagcccc
     3001 gtgcccccgg caaatgcccc ggccgccagc catctcagga gctggacgac cgcctccggg
     3061 tccggcaggg cgtggtcggc cagggtcgga ccctcccacg gcccaccgac gcagatcgac
     3121 cggccgatgc cgcgcaacga cccgaacgcc tcctcgtccg tggcgtcgtc gcccacgtac
     3181 agggcccgga cgcgtgcggg ccagtccgct ccgtgtcgct gcaccaacac gaacagcacg
     3241 gccgccccct tgtgccaggg gaccggcggc cggccctcga ccaccagctt gcccgagacg
     3301 accctgagcc cgcggcgctg catgatcttg gcggcgcgcc gggcggcggg gtcacggccc
     3361 gcttcgagca cgcggcgcac gtgataggaa agggtggccc ctttcgcctc gacctgggcg
     3421 cccggcacgg ccagccgctc cagctccatc gccacccggc tcaccgtgcc cgcgaagcgg
     3481 tcgatgtccg gatgccggta cgagatcccc ggcccctcga tctcgaaccc gtggtttccg
     3541 acgtaggtca gaccgggcac acccacgagg cgccggatgt cggcgagcga ccggcccgtc
     3601 acgatgaccg tatcgaggtt gggcgtgtgg gcggccgatt cgagcgcgag ccgagtcgct
     3661 tgggaaagct gagcgtcttc cggccgctcc tgaatcggcg tcagcgtccc gtcgtagtcg
     3721 aagaacaggg cgaccgtggg ccgggccgcg agccacggct gcaaccggcg gcgcacgcga
     3781 tccaactgcg atacggagcg cggccgggcc gcagcgcgct ccgcggcgtc gaggaagcgc
     3841 accacccagg cctgcacgga gttctcccgc acccgggccc gcagcgcggt catgcgcgcg
     3901 cgccgctcgt ccggcggcat cgtgagggag cggtgcaggg cctcggccat ggcgtccacg
     3961 tcgaaggggt tcaccagcag cgcttcctgc agctcctccg ctgcgcccgc cagctccgag
     4021 aggatcagca cgccgggctc gtcgacctgg gccgcaacgt actccttcgc caccagattc
     4081 atcccgtcgc gcagcggcgt gaccagcgcg acgtcggcgt gccggtacag ggccccgagg
     4141 tccgtggggg acaggctgcg caccaggtag cggatcggcg cccacccctg gtcggagaag
     4201 cggccgttga tccgccccac cgcctcgtcc aattcgcgct tcagcaggcg gtactccgcg
     4261 acctgctcgc gcgacggcac cagcacctgg gtgaacacga aacggccctg aaactcaggg
     4321 ttcgtctcga gcaggcgctc gacggcgagc atgcgctgca ggataccctt ggtgtaatcg
     4381 agccggtcga gaccaatgac gcgggcgggc tccgtcgcgt ccggctgcct gggcccggcg
     4441 gcccgggcga gcgtctcgac gtggcccggg tcgatgccga gcggatggac ctgaatgctc
     4501 acgtcccggc cctcgaagtg cacgctgtcg gtcgcgcggt cgacgtcgca ccccagcagc
     4561 cgctcggcgc aggtgatcag gtgctgggcg tacgccgata cgtgcacccc tacgagatcc
     4621 gcggccagga gcccgcgcag cagctggcgg ctccagggca gaatccggta gacgtcgtaa
     4681 gcgggaaacg ggatgtgcag gaaaaatgcg atgcgccgcc gcggggccag gcgccgcaga
     4741 taatacggga cccgcatcag ctggtagtcg tggatccaca cctggtcttc atcggtgctt
     4801 tcctcggcgg cggcgcgagc gaaccgctcg ttcacctgct cgtacgccgc ccaggtatgt
     4861 gcatcgctgc gcatccggtc gatgaggtaa tggaacaggg gccacagggt gcgattggag
     4921 aagccgccgt agtacgccgc gacttcccga cccgtcaggc tcacggagcg atagcggaca
     4981 cgcgctccgg cggccgggac gagggcgctc gggttgtcgt cgggctcctg ctccagcccc
     5041 ggccagccga cccagacacc gccccgctgg gccagcacgg gatcgagggc cgcgacgagg
     5101 ccaccggagg agcgggtgaa ctggatgccg tcgggccgcc gctcggccgt gaagggcagc
     5161 cggttggaga ccacgacgag cgaccgcgcc cggggcggca ggtcgggaac ggggatgggc
     5221 gccgcaactt gcggcagggt cgtggtcgtc tctggcacgg atatcactcc tcgtcggatc
     5281 agctcgaggg acgttctcgt tactaccacg gtgcagtcta agtggtccgt ggcctgggac
     5341 acactcggga tggtcgccgg ctggcaggcc cctgcgcgcc cggtgcatcg atggcccgga
     5401 aggcggctac gttcccctga ccccggcgcg gtgctcacgt gcgattcgga tcatcgcccc
     5461 gaggctccgc tccacgtcct cgccggttgt tgcccatgac gagatgctga tgcgcatcgc
     5521 ggcccggtcg tgccagaccg taccgccgca ccaacacgtt ccgtccgcct gcagcgcggc
     5581 gatcacccgg cgtgtggtgg cctcgtcgcc gaagctgacg agtacctggt tcagggtcac
     5641 gtcgttcagg atctcgtaac ccgccgcccg cagcccctct gcgaaccgcg tcgcgtggcg
     5701 gcagcagcgc tccaccaggt ccgccagccc gctgcgcccg agggaggcga gcgcggccca
     5761 gatttcgatg ccgcgcgccc ggcgcgacag ctccggcgtg taatcacacg gctcccgggc
     5821 ggcgccgtgc acgagatacg gggccggggt cgacatcgcg ccccgcaacg cggcggcgtc
     5881 gcggaccagc gcgatcccgc tgtcgtaggg gacgttcagc cacttgtggg cgtcgagcgc
     5941 ccacgagtcc gcctcggcga ctccgtccac gagcggcgcc cgtgcgggcg ccgcggcggc
     6001 ccacaggccg aaggccccgt ccacgtgcac ccaggctccc gcggcgtgtg cgatccgaca
     6061 gacgtcccgc acgggatcgc acgctccggt gttcacgttg cccacctgca cgcacacgat
     6121 ggtccggccg tccatcggcg gcagccgttc cgcgcgcatc cggccctgtg catccgtagg
     6181 cacgcgcacc acgcgattgc gcccgagtcc cacgagaccg agcgccttca cgacgctgat
     6241 gtggacctcg tcacccacca ccaccgtcag cggcggcgcg ccgaacagcc cgtcaccctc
     6301 cacgtcccag ccggcccgct cgagcagggc gtggcgggcg gccgcgagtc ccgtgaagtt
     6361 cgccatggtc gcgccggtga cgaacccggc ggcgcaatcg ggcgggaggt cgagcgcctc
     6421 gagcagccac cgcatggcga cctgctcgaa tgccgccact cccggcccga gcacgacgag
     6481 attcgcgtcc tgatcccagg ccgacgcgag ccagttggcg gcgagcgtca ccggcagcgt
     6541 gccgccgatc acgaacccga agtagcggcc gccggcgctc gcggtcgtcg ccggagaggc
     6601 gacccggtgg agctcggtga gcacggcgag cggatcggcc ggcctgtcgg gcaggggacg
     6661 gtcgatcgcg cgcagcgcgt cgagggcggc gtgcgtgggg gcgacactac gggtggggag
     6721 cgtctccaga tactcggccg cgtacccggc cgccgtctgc agcaggtgtc ggtcgtccat
     6781 ggcgcaggaa gatagccacc ccgaggattg acctgccggc ccgtccccga ctagcctccg
     6841 aggctatggc cagcgactcg ctgatcgtcc gcggcgcgcg cgagcacaac ctcaagaaca
     6901 tct
//
LOCUS       DBNGZW010000444         2935 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_927727, whole genome shotgun sequence.
ACCESSION   DBNGZW010000444 DBNGZW010000000
VERSION     DBNGZW010000444.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2935)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2935)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2935
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_927727"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..552)
                     /locus_tag="AC16JC_10535"
     CDS             complement(<1..552)
                     /locus_tag="AC16JC_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HIR5165172.1"
                     /translation="MTITAHSLFASPNALAPHYSRFRVAERLLLTGHSHQAWPDVGFA
                     AQQQAWLDAAELVDDKWDRAFAMADRVRGGFARLLDDADGDIALGANTHEMVVRFLSA
                     LPLKQRPRLVTTDGEFHTIRRQLDRLYEAGIEIVRVPARPADQVAERLARMVTDRTAA
                     VLVSAVLYENAHIVPNLGITLEAS"
     gene            complement(549..1331)
                     /locus_tag="AC16JC_10540"
     CDS             complement(549..1331)
                     /locus_tag="AC16JC_10540"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - L-tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HIR5165173.1"
                     /translation="MAVTYASYLRLDELLALQRPHEGELEHDETLFIIIHQVYELWFK
                     EVLHELDHLTRLLRDNATPQALHTLKRILTVLKVLVAQIDILETMNPLGFLSFRDRLE
                     SGSGFQSFQFRELEFVLGLKQTSAVERYPDGSRHRERVERRYNEPGLWDAFLVFLQHH
                     EYQVPRALLERDLTRPIEPSPELQRVLIRVYRTDPTVTAVCERLLDLDEGFMEWRYRH
                     VKMVQRTIGTRRGTGGSAGADYLLTTLNRPAFPDLWEIRTEL"
     gene            complement(1344..>2935)
                     /locus_tag="AC16JC_10545"
     CDS             complement(1344..>2935)
                     /locus_tag="AC16JC_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020741089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR5165174.1"
                     /translation="IAFCQELFAAELAGHRLQRNRSIWRSFGTVRNECWHHENIVLIG
                     DSAHTAHFSVGSGTKLAMEDAIVLVRALGNSADLPAALVAYETERRPVVESLQRAAQV
                     SLQFFENTERYMDQDPVQFGFNLLTRSLRITHDNLKVRDAEFVAGVDRWFAAGAARQA
                     RVAVPLEPPPPPLFTPYRLGELLLENRSVVSPMCQYCAEDGVPNDWHLVHLGTRALGG
                     AALVFTEMTDVTREARISTGCTGMYKPEHVAAWSRIVDFVHRHTRAKIGLQLGHAGRK
                     AATKKMWEGDNQPLKEGAWPIMAASPIPYYPHSQVPREMNRGDMDDVSAAYVRATRMA
                     EAAGFDALELHYAHGYLMAGFISPLTNRRRDEYGGPVENRMRFPLEVFDAVRAAWPAA
                     KPISVRISAVDWAPGGMESQDAVDVARLLREHGCDILDVSAGQTVADQRPVYGRLFQT
                     PFADRIRHEAGMPVMTVGNISSFTDVNTILAAGRADLCVLARAHLYDPYWTRHAAQAL
                     GWPMEWPDQYHTLNRYKPRFI"
BASE COUNT          462 a          962 c         1040 g          471 t
ORIGIN      
        1 ggaggcctcg agggtgatcc ccaggttggg cacgatgtgg gcgttctcat acagcacggc
       61 tgacacgagc accgcggccg tgcggtcggt taccatccgg gcaagacgct cggcaacctg
      121 gtcggcgggt cgagccggaa cgcgaacgat ttcgatgcct gcttcgtaga gccgatcgag
      181 ctgacgccgg atcgtgtgga attcgccgtc ggtcgtgacg aggcgggggc gctgcttcag
      241 tgggagagcg gagagaaagc gcacgaccat ctcgtgggtg ttcgcgccga gtgcgatatc
      301 cccgtctgcg tcgtcgagca gccgggcgaa gccgccccgc acgcggtcgg ccatcgcgaa
      361 ggcccggtcc catttgtcgt ccaccagctc ggcggcgtcg agccaggcct gctgctgcgc
      421 cgcgaatccg acgtcgggcc aggcctgatg cgagtggccc gtcaggagca gccgctcggc
      481 gacgcgaaag cgggagtagt gtggcgccag cgcgttgggg gacgcgaaga gcgagtgcgc
      541 ggtgatggtc acagttcggt gcggatctcc cacagatcgg ggaacgcggg ccggttgagg
      601 gtcgtcagga ggtagtccgc gcccgcggag ccgcccgtgc cgcgccgcgt cccgatcgtg
      661 cgctgcacca tcttcacgtg gcgatagcgc cactccatga acccctcgtc gagatcgagg
      721 agccgctcac acaccgccgt gacggtggga tcggtgcggt acacgcgaat cagcacgcgc
      781 tgcagctcgg ggctcggctc gatcggccgg gtgagatccc gttcgagcag cgcccggggg
      841 acttgatact cgtggtgctg gaggaagacg aggaacgcgt cccacagccc cggctcgttg
      901 tagcgccgct cgacccgctc gcggtggcgg ctgccatcgg ggtagcgctc cacggcgctc
      961 gtttgcttca gcccgagcac gaattcgagc tcgcggaact ggaacgactg gaatccgctg
     1021 cccgattcga gacggtcccg gaaggagagg aacccgagcg ggttcatggt ctccaggatg
     1081 tcgatctggg ccaccagcac cttcagaacg gtgagaatcc gtttgagggt gtgcagagcc
     1141 tgcggggtcg cgttgtcgcg cagcagacgc gtcaggtggt cgagctcgtg cagcacttcc
     1201 ttgaaccaca gctcgtacac ctgatgaatg atgatgaaca gcgtctcgtc gtgctcgagc
     1261 tccccctcgt ggggacgctg cagggcgagc agctcgtcga gccgcagata actcgcgtag
     1321 gtcacggcca tgatcgttcg tccctagatg aagcgcggtt tgtagcgatt gagcgtgtgg
     1381 tactggtccg gccactccat cggccacccg agcgcctggg cggcgtgacg ggtccagtac
     1441 ggatcgtaga gatgcgcccg cgccagcacg cacaggtcgg cgcggccagc cgcgaggatg
     1501 gtgttgacgt cggtgaagct ggagatgttg cccacggtca tcaccggcat ccccgcctcg
     1561 tggcgaatcc ggtcggcgaa cggggtctgg aacagccgcc cgtacacggg ccgctggtcg
     1621 gccaccgtct ggcctgccga gacgtcgagg atgtcgcagc cgtgctcccg gagcagccgc
     1681 gccacgtcca ccgcgtcctg cgattccatc ccgcccgggg cccagtccac ggcggagatg
     1741 cgcaccgaga tgggtttggc cgcgggccag gcggcgcgga cggcgtcgaa tacctcgagg
     1801 gggaaccgca tccggttctc caccgggccg ccgtattcgt cccggcgccg gttggtgagc
     1861 ggcgagatga agccggccat caggtaaccg tgcgcatagt ggagctccag agcatcgaac
     1921 ccggccgctt cggccatgcg ggtcgcgcgc acatacgccg cgctcacgtc gtccatgtcg
     1981 ccgcgattca tctcccgcgg cacctggctg tgcgggtaat agggaatggg cgacgccgcc
     2041 atgatcggcc atgccccctc cttgagcggc tgattgtcgc cctcccacat cttcttggtc
     2101 gcggccttgc ggccggcgtg gcccagctgg aggccgatct tcgcccgcgt gtgacggtgc
     2161 acgaaatcga cgatccggct ccaggcggcg acgtgctccg gtttgtacat cccggtgcag
     2221 ccggtcgaaa tgcgcgcctc gcgcgtcacg tcggtcatct cggtgaagac gagcgccgcg
     2281 ccgcccagcg cgcgtgtccc caggtggacg aggtgccagt cgttgggcac accgtcctcg
     2341 gcgcaatact ggcacatggg agagacgacg ctgcggttct cgagcagcag ctcccccagc
     2401 cggtacggcg tgaacagagg cgggggcggc ggctcgagcg gcaccgcgac gcgggcctgc
     2461 cgggcggcac cagccgcgaa ccagcgatcc acgccggcca cgaactccgc atcgcggacc
     2521 ttgaggttgt cgtgcgtgat gcgcagcgag cgggtgagca gattgaaccc gaactggacc
     2581 ggatcctggt ccatgtagcg ctcggtgttc tcgaagaact gcaggctcac ctgggcggcg
     2641 cgctgcaggc tctcgaccac gggccgccgc tcggtctcgt acgccacgag cgccgccggc
     2701 aggtcggcgc tgttgcccag cgcgcgcacc agcacgatcg cgtcttccat cgcgagcttc
     2761 gtgcccgagc cgaccgagaa gtgcgcggtg tgggccgagt cgccgatcag cacgatgttc
     2821 tcgtggtgcc agcattcgtt gcgcaccgtg ccgaagctgc gccagatgga gcggttccgc
     2881 tgcagccggt gcccggcgag ctctgcggcg aacagctcct ggcaaaaggc gatcg
//
LOCUS       DBNGZW010000445         4336 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_586882, whole genome shotgun sequence.
ACCESSION   DBNGZW010000445 DBNGZW010000000
VERSION     DBNGZW010000445.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4336)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4336)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4336
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_586882"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..553
                     /locus_tag="AC16JC_10550"
     CDS             164..553
                     /locus_tag="AC16JC_10550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013707118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5165175.1"
                     /translation="MPGATVRSYRDLLVWQRSLAIALRIYDATVPFPRSELYGLVAQM
                     RRAAVSIPSNIAEGHGRHHLGEYLHHLSIANGSLMELETQVIISSRRGYLSAQEVRAL
                     LVSCAEVGRMLAGLTRALSRGRGRAAR"
     gene            complement(578..3049)
                     /locus_tag="AC16JC_10555"
     CDS             complement(578..3049)
                     /locus_tag="AC16JC_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR5165176.1"
                     /translation="MDTLLQDLRYALRTLSRSPAFTASAVLTLALGIGAATAGFTLLD
                     WVTLRPVPGIREPSRAGFVEFAVQDEHGYRPNGLTISQRDLVLRASPAASGLAGREGP
                     MAVNAAAEMSAAQRVSAEFVTSDYFTTLGVATAHGRGFVAEDEAPPLGNRVAVISDRL
                     WRDLFAARSDVLGLPLRVNGLPCTVIGVAPPGFRGPDLFDPADIWMPGATEWDIRHLD
                     PTRRRAESHYRFVLRLRPGASFDQAERQLSAAVRAVAVADTAHFSPQVSAQVLPGLGL
                     DALHGAKEGIDHQLQLIIGIAALVLLVACANVANLLLLRRAQRRSDAVVRLALGASRF
                     RLVRYALTESVLIGVASGILGVLVALWVNGLFHRFRLLRFISMEGLRLDWRVIGFAAA
                     AGLLAAVFAALLPALVGSRADLVGELKASGPTQAGGAPLLRTGLAVLQVAVSLTLVAG
                     TYLFGRTLQRYAQVPLGFDPAGVTVFQVDPMAQGYTPQQARNYLQALRARVAGLPGVE
                     TAAFASLTPFVGIFNIDAVRRSDAPTDAKPIGAASQQVSGDYFSALRIPILHGSTFQP
                     SDVWPDSAQRVGKVILSAALARKVFGTTEPVGRLVVLPFHRENGTAEVVGVVGDVHWN
                     ERGGDNDPILYSPVGQQATPYGPKLVVRSRLSASTLEHQTQLIGRRLDPAMPIESNGP
                     LSDAVGAAISSQTVLFRLAGLLSAVALLLAAVGVYSLIAYGITTRVREFGVRMALGAE
                     VRDILRTAARPAVTIIALGVVCGIAGAMYLTRFIKASLYGVSPLDPVAFVTAAVVLGF
                     AVLLASWLPARRASKVDPMVALRYE"
     gene            complement(3132..>4336)
                     /locus_tag="AC16JC_10560"
     CDS             complement(3132..>4336)
                     /locus_tag="AC16JC_10560"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR5165177.1"
                     /translation="RATSAHHRSRLRSALVVGEVALSLVLLAGGALLVRTFANLLRSD
                     TGFEPGHVLSAEIWLTGSRYDSTAAIAGFYGELTRRLEALPGVEQAAVVEAGLPLVRG
                     GNLPVTVDGEFHSTDYRTVTPGYFATLRIPLVAGRVLEAGDAEHAEPVVVVNRSFAHR
                     YMADSALGRIVKVGGSDQNPYRRIVGVVGDVKSFVGFPAPPTVFITSSQTPAGFTRIF
                     GSWFPTHVLVRATGDPRMLRTTLARVIHEADAQVPVGQVRSMPEVLATSLASQRFIMT
                     LLSVFAGLAVVLAAVGLYGVLSYLVGQRTHEIGVRVALGARPHQVLALVLARGLVLAG
                     TGVALGLMGAAAATRLLASQLYGVRPTDPLTLGAVTLLLGIVALLACVVPAWRGTRVD
                     PMVALRSE"
BASE COUNT          744 a         1477 c         1522 g          593 t
ORIGIN      
        1 gaggagtcgc ggtgaggaat ttggcacggt gctaaaggtg tcgggtgtcg ggtgtcgggt
       61 gccaggtggt gcgcagaaat gctcctcgcg gggtgattct gatgatgacc ccctgcaagt
      121 gccgtgagag tttgagggcg tttctcaacg acgatgggtg accatgccag gggcgaccgt
      181 acgaagttat cgggatcttc ttgtgtggca gcgatcgctt gccatcgcgc tgagaatcta
      241 cgacgccaca gtccctttcc cgcgcagtga gctctacggc ctggtggccc agatgcggag
      301 ggcggcggtg tcgataccgt ccaatatcgc tgaaggccac gggcgtcacc accttggtga
      361 gtatctgcat catctttcga tcgcaaacgg ttcgctcatg gagctggaaa cgcaagtcat
      421 catctcctcg cggcgaggat acctgtcggc tcaggaggtg cgtgcgctcc tcgtgtcctg
      481 cgccgaagtc ggccggatgc tggctggttt gacacgcgcg ctcagccgcg gtcggggacg
      541 cgccgcacgc tgacacctgg aacccggcac ctggcaccta ttcgtatctc aacgccacca
      601 ttgggtcgac cttcgaagcg cgacgagctg gaagccagct cgccagcagc acggcaaatc
      661 ctagaaccac cgccgccgtc acgaacgcga ccgggtcgag cggactcacg ccgtacagcg
      721 acgccttgat gaagcgggtc aggtacatcg cgccggcgat gccgcaaacg acacccaggg
      781 cgatgatggt gaccgccgga cgcgcggcgg tgcgcaggat gtcccgcact tcggcgccga
      841 gcgccatgcg cacaccgaat tcccggacgc gggtcgtgat cccgtacgcg atgagcgagt
      901 aaacgccgac ggcggcaagg agcagcgcga cagcggagag caggcccgcg agcctgaaca
      961 ggacggtctg gctcgagatc gccgccccca cggcgtcgct caaggggcca ttggactcga
     1021 tcggcattgc cggatcgaga cgccggccga tcagctgcgt ctggtgttcg agcgtcgaag
     1081 cgctgaggcg gctgcgaacg acgagcttcg ggccgtacgg ggttgcctgt tggccgactg
     1141 gactgtagag gatcggatcg ttgtctccgc cccgctcgtt ccagtgcacg tcaccgacga
     1201 cgcccacgac ctcggcggta ccgttctccc ggtggaacgg caacaccacc agccgtccga
     1261 cgggctccgt ggtgccgaac accttgcgcg ccagcgcggc gctgaggatg accttcccca
     1321 cgcgttgcgc ggaatcgggc cacacgtcgc tgggctggaa ggtcgagccg tggaggatcg
     1381 ggattctcag ggccgagaag taatcccccg atacctgctg ggatgcggcg ccgatgggtt
     1441 tggcatcggt cggcgcgtcc gaccgccgca cggcatcgat gttgaagatt ccgacgaacg
     1501 gggtcaggct cgcaaaggcg gccgtctcga ccccgggcag ccccgcgacg cgcgcccgga
     1561 gtgcctgcag gtagttgcgg gcctgctgcg gcgtgtaccc ctgcgccatc gggtccacct
     1621 gaaacaccgt cacgcccgcg ggatcgaagc cgaggggcac ctgcgcgtag cgctgcaggg
     1681 tgcgcccgaa caggtacgtc cccgccacca gcgtcagtga aacggcgacc tgcagcacgg
     1741 ccaaccccgt gcgcagcagc ggggcgcctc cggcctgcgt cgggccggac gccttgagct
     1801 cgccgaccag gtcggcccgg gagccaacca gcgccggaag cagcgcggca aagacggccg
     1861 cgagcagtcc ggccgcggcg gcgaatccga tcacccgcca atcgagtcgc aggccttcca
     1921 tcgagatgaa tcgcagcaac cggaaccggt gaaacagtcc gttgacccac agcgcgacga
     1981 gcaccccgag gatgccgctc gccaccccga tcaggacact ctcggtcagg gcgtaccgca
     2041 ccaggcggaa ccggctggca ccgagagcga ggcggacgac cgcatcgctg cggcgctgcg
     2101 cgcgacggag cagcaggagg ttggcgacgt tggcgcaggc gacgagcagg acgagcgccg
     2161 caatgccgat gatgagctgg agctggtgat cgatgccctc tttcgcgccg tgcagcgcgt
     2221 cgagcccgag gccggggagc acctgggccg agacctgcgg tgagaagtgc gcggtgtcgg
     2281 cgacggccac ggcgcggacc gcggcgctca gctgtcgctc cgcctgatcg aatgaagcgc
     2341 ccggccgcag tcggagcacg aagcggtagt gggactccgc ccggcggcgc gtcgggtcga
     2401 gatggcggat gtcccactcg gtggcgcctg gcatccagat gtcggccgga tcgaacaggt
     2461 ccgggccgcg gaatcccggc ggagcgaccc cgatcaccgt gcagggcagc ccgttcaccc
     2521 ggagcggcag tccgaggacg tcggagcgcg cggcgaacag gtcgcgccac aaccggtcgc
     2581 tgatcacggc gacccgattg ccgagcgggg gtgcttcgtc ctcggccacg aatccccggc
     2641 cgtgcgccgt ggcgaccccg agcgtcgtga agtaatcgct cgtcacgaac tcagccgaga
     2701 cgcgttgcgc ggcgctcatc tccgcggcgg cgttcaccgc catcggacct tcgcggcccg
     2761 ccaatccgct cgcggcgggt gaggcgcgga gcacgagatc gcgctgcgag atcgtgagcc
     2821 cgttgggccg gtacccgtgc tcgtcctgta ccgcgaactc cacgaacccg gcgcgggacg
     2881 gctcgcgtat acccggcacg gggcgcaacg tcacccagtc caagagggtg aatcccgccg
     2941 tggccgcgcc gatcccgagc gcgagcgtga gcaccgcgct ggcggtgaag gcggggctcc
     3001 gcgagagggt gcggagcgcg tagcggaggt cttgcagcag ggtgtccatt gtgcctccat
     3061 gcaggtgccc ggttccaggt gtctggtgtc agggtgaaac cacctgacac ctggcccctg
     3121 gcacccggca tctattcact tcgcagcgcc accatcggat ccacccgcgt tccccgccac
     3181 gccggcacga cgcaggcgag gagggcgacg atccccagca ggagcgtcac ggcgccgagc
     3241 gtcagcggat cggtcgggcg cacgccgtac agctggctgg cgagcaagcg tgtcgccgcc
     3301 gccgcgccca tcagacccag cgcgacaccc gtccccgcca ggaccaggcc gcgcgcgagc
     3361 acgagagcca gaacctggtg cggccgcgcc cccagcgcca cgcgcacccc gatctcgtgg
     3421 gtgcgttggc cgaccaggta ggacaacacg ccatacagcc cgaccgcggc cagcaccacc
     3481 gccaggccgg cgaacacgga gagcagggtc atgatgaatc gctgagatgc gagcgaagtg
     3541 gcgagcacct cgggcatcga gcggacctgc cccaccggga cctgggcatc ggcctcatga
     3601 atgacgcgag cgagagtggt ccgcagcatc cgtggatccc ccgtcgcacg gacgaggaca
     3661 tgcgtgggga accagctgcc gaagatgcgg gtgaacccgg ccggcgtctg cgacgaggtg
     3721 atgaagaccg tcgggggggc ggggaaaccg acgaacgatt tgacgtcgcc cacgaccccg
     3781 acgatgcggc gatacggatt ctggtcgctg ccgccgacct tgacgatccg tcccagcgcg
     3841 gaatcggcca tataccggtg cgcgaacgac cgattcacga ccacgaccgg ctcggcgtgc
     3901 tccgcgtcgc cggcttccag cacgcgcccg gcgacgagcg ggattcgcag ggtcgcgaag
     3961 tagcccgggg tgacggtgcg gtagtccgtg gagtggaatt cgccgtccac cgtgaccggc
     4021 aggttgccac cgcgcacgag cggtaatccc gcctcgacga ccgccgcctg ctccacgccc
     4081 ggaagggcct cgaggcgccg ggtcagctcc ccgtagaagc ccgcgatggc cgccgtcgag
     4141 tcgtagcgcg acccggtgag ccagatctcc gccgagagga cgtggccggg ctcgaacccc
     4201 gtatcgctgc gcagcaggtt ggcgaacgtt cgcaccagca gcgccccacc cgccagcagc
     4261 acgagggaga gcgcgacctc gcccacgacc agcgcactgc gcaaccggct gcggtggtgg
     4321 gcgctggtgg cgcgcc
//
LOCUS       DBNGZW010000446         2554 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_712407, whole genome shotgun sequence.
ACCESSION   DBNGZW010000446 DBNGZW010000000
VERSION     DBNGZW010000446.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2554)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2554)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2554
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_712407"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1313)
                     /locus_tag="AC16JC_10565"
     CDS             complement(<1..1313)
                     /locus_tag="AC16JC_10565"
                     /inference="COORDINATES: protein motif:HMM:NF015935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HIR5165178.1"
                     /translation="MSVERLAPEVQDLLRAEIVAARGREVSFVARTDGRGAIVEARVV
                     ARGTVDAVLALPGSARRGEMLLHNHPSGELEPSGADLSVAARLHDDGVGFGIVNNEVT
                     ELYVVVECPRAKSLERLDPVTTARLLGVDGPVADAVGREGFEDRPAQRDMAAYIADAY
                     NDGGVALLEAGTGVGKSFAYLVPALLWGQANAERTVVSTNTINLQEQLVGKDLPVLAR
                     ALATPDHEPTFALLKGWRNYLCLARLEQARGAQETLFETNRSAELEGIAAWAARSADG
                     SLADLADEPSEDVWDAVAAESDLCTRLKCPHFERCFVFQARRRAAEADVVVVNHHLLA
                     SDLAVRLAADNWMEAAVLPPYRRLILDEAHHLEDVAAAHLGAQVSQMGVQRLLGRLER
                     GGRGLMPALHAELSARDDLLSAASRDLLEQRLLDALRAARRWSEEL"
     gene            complement(1310..>2554)
                     /locus_tag="AC16JC_10570"
     CDS             complement(1310..>2554)
                     /locus_tag="AC16JC_10570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HIR5165179.1"
                     /translation="LPPPPPPPPPPATADSSLLIPMDDAQSDHLKAYGVAYRVIQAGG
                     KAEWLLYYRGGSFLLPDAPTIRRDAALNGVAVEPVDGGQVSAVRGEIAGTNADTVPLE
                     KAPKVAVYVPPNAQPWDDAVTMALQYAGIPFEKLWDPEVLGDGLRTYDWLHLHHEDFT
                     GQYSKFFLIYSGQSWLAEMVRFNDAARRRLGFASVPDMKKAVAGAIRAYVDRGGFLFA
                     MCTATETIDLALAAHDVDAAASFADGTPMDPAADSKLDWSRTFAFSGAHLEPDPQVAS
                     FSDIDGHQVNAGARRQPLGAFKLFNFSAKIDPVPTMLVQNHRAVVPDFYGLTTSFMRE
                     RLKPGVTILADEEGAPWVKYIHGDLGKGTWTFFGGHDPEDPQHQIGDPPTDLSLHPHS
                     PGYRLILNNVLFPAAKKRELKT"
BASE COUNT          372 a          920 c          894 g          368 t
ORIGIN      
        1 agctcttccg accagcgccg cgcggcccgc agcgcgtcga gcagccgctg ctccagcagg
       61 tcccggctcg ccgcggagag caggtcatcg cgggccgaca gctcggcgtg cagcgccggc
      121 atcaggcccc gcccgccccg ctcgagccgc cccagcagcc gctgcacgcc catctgcgag
      181 acctgggccc cgaggtgcgc cgcggcgacg tcttccagat gatgggcctc gtcgaggatg
      241 agccggcggt acggcggcag cacggccgcc tccatccagt tgtccgccgc caggcgcacg
      301 gcgagatcgg aggcgagcag gtgatggttc accaccacga cgtcggcctc ggccgcgcgc
      361 cgccgcgcct ggaacacgaa gcagcgctcg aagtgcgggc acttgagccg cgtgcacaga
      421 tcggattccg ccgcgaccgc gtcccacacg tcctcggacg gctcgtcggc caggtcggcg
      481 agactgccgt cggcgctgcg cgccgcccac gcggcgatcc cctccagctc cgcggaccga
      541 ttcgtctcga acagcgtctc ctgcgcgccc cgcgcctgct cgaggcgggc gaggcagagg
      601 taattccgcc accccttgag cagcgcgaac gtcggctcgt ggtcgggcgt ggccagggcg
      661 cgcgccagca ccggcagatc cttgccgacc agctgctcct gcaggttgat ggtgttggtc
      721 gagacgacgg tgcgctcggc gttcgcctgt ccccacagga gcgcgggaac gagatacgcg
      781 aacgacttgc ccacgcccgt gccggcctcg agcagcgcca cgccgccgtc gttgtacgca
      841 tcggcgatgt aggccgccat gtcgcgttgc gcgggccggt cttcgaaccc ttcccgcccc
      901 acggcgtcgg ccaccggtcc atccacgccc aggaggcggg ccgtcgtcac cggatcgagc
      961 cgctccaggc tcttggcgcg cggacattcc accacgacgt agagctcggt cacctcgttg
     1021 ttcacgatcc cgaacccgac gccgtcgtcg tgcagccgcg ccgccaccga cagatcggcc
     1081 ccggacggct cgagctcccc gctggggtga ttgtgcagca gcatctcgcc ccgccgcgcc
     1141 gaccccggca gcgcgagcac ggcgtccacg gtgccccgcg ccacgacgcg cgcttcgacg
     1201 atggcgccgc gcccgtcggt ccgcgccacg aacgacacct cacgcccccg cgccgcgacg
     1261 atctccgcgc gcagcaggtc ctgcacctcg ggcgcgagcc gctcgacgct cacgtcttca
     1321 gctcacgctt tttcgcagcg ggaaacagga cgttgttgag gatcagccgg tagcccggag
     1381 agtgcgggtg gagcgagagg tcggtcggag gatcaccgat ctggtgctgg gggtcttccg
     1441 ggtcgtgccc gccgaaaaag gtccaggtgc ccttgcccag gtcgccgtgg atgtacttca
     1501 cccagggcgc cccctcctcg tcggccagga tggtgacgcc gggcttcagg cgctcgcgca
     1561 tgaaggacgt ggtgagtccg tagaagtccg gaaccaccgc gcggtggttc tgcaccagca
     1621 tcgtcggcac cgggtcgatc ttggcgctga aattgaacag cttgaacgcg ccgagcggct
     1681 gccgccgcgc ccccgcgttc acctggtgcc cgtcgatatc cgagaaactg gcgacctgcg
     1741 ggtcgggttc caggtgggcg cccgagaacg cgaacgtccg gctccagtcg agcttcgagt
     1801 ccgcggcggg atccatcggc gtgccgtcgg cgaagctggc ggcggcatcc acgtcgtgcg
     1861 cggccagggc gaggtcgatc gtctcggtcg ccgtgcacat ggcgaacagg aacccgccgc
     1921 gatccacata ggcccggatg gcgcccgcga ccgccttctt catgtcgggc accgaggcga
     1981 agccgagccg ccggcgcgcc gcgtcgttga agcgcaccat ctcggccagc cacgactggc
     2041 cggaatagat gaggaagaat ttggagtact ggccggtgaa gtcttcgtga tgcaggtgca
     2101 gccagtcgta ggtgcgcagg ccgtcgccga gcacttccgg gtcccagagc ttctcgaacg
     2161 ggatgccggc gtactgcagc gccatcgtca ccgcgtcgtc ccacggctgc gcgttgggcg
     2221 ggacgtacac cgcgaccttg ggcgccttct ccaggggaac cgtgtccgcg ttcgtgcccg
     2281 cgatctcgcc ccgcaccgcg ctcacctgcc cgccatccac cggctcgacc gccactccgt
     2341 tgagcgcggc atcgcggcgg atcgtcggcg cgtcggggag caggaacgat ccgccacgat
     2401 agtagagcag ccactcggct ttgccgccgg cctggatgac gcggtaggcg acgccgtatg
     2461 ccttgaggtg gtcggactgc gcgtcgtcca tgggaataag gagagacgaa tcggcggtcg
     2521 cggggggggg gggggggggg gggggagggg ggag
//
LOCUS       DBNGZW010000447         3311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_712494, whole genome shotgun sequence.
ACCESSION   DBNGZW010000447 DBNGZW010000000
VERSION     DBNGZW010000447.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3311
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_712494"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..575)
                     /locus_tag="AC16JC_10575"
     CDS             complement(<1..575)
                     /locus_tag="AC16JC_10575"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1/NuoH family protein"
                     /protein_id="HIR5165180.1"
                     /translation="MTFFLVSSLIKVVLVFFAILIGVALLTWLERRVCAWMQDRMGPN
                     RVGPFGLLQPAADGLKNIVKEETYPATADLWTFVLAPAVAFVPALLTFAVIPFGTPLP
                     TRWGPVPFVVADLPVGFLYILAIGSLGVYGIVLAGWSSNNKYALLGGLRASAQLISYE
                     IAMGMSTVAVLLLAGNVSLSDVVARQQGSVF"
     gene            complement(572..2143)
                     /locus_tag="AC16JC_10580"
     CDS             complement(572..2143)
                     /locus_tag="AC16JC_10580"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024900.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit G"
                     /protein_id="HIR5165181.1"
                     /translation="MVSLTIDGVPVSVPPGTLVIEAAKQAGVLVPHYCYHPGLPAGYQ
                     LSIPAAGVCRMCLVKIEKTPKLQIACAVQVAEGMVVETQAPEAKAQRKGVLEFLLINH
                     PLDCPICDQAGECELQDFTFQEGRPETRYTPTYPKRFNPVEDFGPDVLYVPNRCIYCV
                     RCVRFMQDVAKEPVLNVSERGDRAYIGIHPDARLDHPWAGNVVDLCPVGALLSKDFLH
                     KARAWELDKSASICTGCSQGCNVTLDMRDSTVVRVRPRPNLDVNQYFICDHGRANYRW
                     MNRGDRIEAPLVRINGELVATDWDTALDRVRALVRGVGGRAVTVASPRASTEALFLGR
                     RLLSRFDWTGGVQVVMGEEAPLAGVPNLALRPERAPNGTGAGLLGYTPDIAAALSAAS
                     AAGLVLVLDEPDVPVETKGVLIYAGTVLPAAARSAEVVLPLANMAEEDGTYVNRDGRV
                     QRYLQAKPAPGMARPAWWALGELVAELEGSPHPGSASDVFDALAASVEAFGGLSYGAL
                     GLQGRRVTAGAAVTA"
     gene            complement(2176..>3311)
                     /gene="nuoF"
                     /locus_tag="AC16JC_10585"
     CDS             complement(2176..>3311)
                     /gene="nuoF"
                     /locus_tag="AC16JC_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR5165182.1"
                     /translation="VIEEVKNSGLRGRGGAGFPTGLKWSFMPKEKKKAHYLCVNADES
                     EPGTFKDREIMLWTPHALLEGTVIAAHAIRAKTVYIYVRGEFTEPLAILEQAIAEANA
                     AKLFGDIRMFLHRGAGAYICGEEKALMNSIEGKRGNPRIKPPFPAAAGVFGMPTTINN
                     VETLAAVPHILRHGAAWYKKMSLSNPKSTGTKLFSVCGHIARPGNYEVTMGFPLKDLL
                     YELAGGMRPGRTLKACIPGGSSVPILNREETESTLLDYEGCVEKGTMLGSGGVIVFDD
                     SADMVYQIMRLAKFYAHESCAQCTQCREGTAWTVKILERILSGQGAMKDLDLLLELWQ
                     NMTGTTICVLSDSCAAPVASGIKKFRSEFEDYIAGRRKPVLAA"
BASE COUNT          518 a         1208 c         1086 g          499 t
ORIGIN      
        1 tggaacaccg acccctgctg gcgcgccacg acgtcgctga gcgacacgtt cccggcgagc
       61 agcagcaccg ccaccgtgct catccccatc gcgatctcgt aggagatgag ctgcgccgag
      121 gcccgcagcc cgcccagcag cgcgtacttg ttgttcgacg accaccccgc cagcacgatg
      181 ccgtacacgc cgagcgaccc gatcgccagg atgtagagga atcccaccgg caggtcggcc
      241 accacgaacg gcaccgggcc ccaccgggtg ggcagcggcg tgccgaacgg aatcaccgcg
      301 aaggtgagca gcgccggaac gaacgccacc gccggcgcga ggacgaacgt ccacaggtcc
      361 gccgtggcgg gataggtctc ctcctttacg atgttcttca ggccgtcggc cgcgggctgc
      421 agcaacccga acggccccac gcggttcggc cccatccggt cctgcatcca ggcgcagacc
      481 cggcgctcga gccaggtgag cagcgccacc ccgatcagga tcgcgaagaa gaccaggacc
      541 accttgatga gcgaggagac gaggaagaac gtcatgccgt caccgccgcg cccgccgtga
      601 cccggcggcc ctgcagcccc agcgcgccgt aggacaggcc gccgaacgct tccaccgacg
      661 cggcgagcgc gtcgaacacg tccgacgccg agccgggatg cggcgagccc tcgagctcgg
      721 ccaccagctc gcccagcgcc caccatgcgg gccgcgccat ccccggggcc ggcttggcct
      781 ggaggtaacg ctgcacgcgc ccgtcgcggt tcacgtaggt cccgtcctcc tcggccatgt
      841 tcgccagcgg cagcacgact tcggcgctgc gcgccgccgc cggcagcacc gtgccggcgt
      901 agatgagcac gcccttcgtc tcgaccggga cgtccggctc gtccagcacc agcacgagcc
      961 cggcggccga ggccgccgac agcgccgccg cgatgtcggg ggtgtagccc agcagccccg
     1021 ccccggtgcc gttgggtgcg cgctccggcc gcagcgccag gttgggcacg cccgcgagcg
     1081 gcgcctcctc gcccatcacg acctgcacgc cgccggtcca gtcgaaccgc gacagcagcc
     1141 tgcgcccgag gaacagggct tcggtggacg cccgcggcga cgccaccgtc acggcccgtc
     1201 cacccacgcc gcgcaccagc gcgcgcacgc ggtcgagcgc cgtatcccag tcggtggcca
     1261 cgagctcgcc gttgatgcgg accagcggcg cctcgatgcg gtcgccgcgg ttcatccagc
     1321 ggtagttggc gcggccgtgg tcgcagatga agtactggtt cacgtcgaga ttgggccggg
     1381 gtcgcacccg caccaccgtg ctgtcgcgca tgtcgagcgt cacattgcac ccctgcgagc
     1441 agccggtgca gatcgacgcc gacttgtcca gctcccaggc gcgcgccttg tgcaggaagt
     1501 ccttcgagag gagcgcgccc accggacaga gatccaccac gttccccgcc caggggtgat
     1561 cgagccgggc atcgggatgg atcccgatgt aggcgcggtc gccgcgctcc gacacgttga
     1621 gcaccggctc cttggccacg tcctgcatga agcgcacgca gcgcacgcag tagatgcagc
     1681 ggttgggaac gtacaggacg tcgggcccga aatcctcgac cgggttgaag cgtttgggat
     1741 aagtcggcgt gtagcgcgtc tcgggccggc cttcctggaa ggtgaagtcc tgcagctcgc
     1801 actcccccgc ctgatcgcag atgggacaat cgagcgggtg attgatgagc aggaattcca
     1861 gcacgccctt gcgctgcgcc ttcgcctcgg gggcctgcgt ctccaccacc atgccctcgg
     1921 ccacctggac cgcgcaggcg atctgcagct tgggcgtctt ctcgatcttc acgagacaca
     1981 tgcggcagac gccggccgcg gggatgctga gctgatagcc ggcgggcagc cccggatggt
     2041 agcagtagtg cggcaccagc acgccggcct gcttggcggc ctcgatgacc agcgtgccgg
     2101 gaggcacgct caccgggacg ccgtcgatcg tcagggaaac catgccgtca gccatccgcc
     2161 gtcagcgccc cctcgttacg cggcgaggac cggcttgcgc cggcccgcga tgtagtcctc
     2221 gaactcgctc cggaacttct tgatcccgga cgccaccggc gcggcgcagg agtcggacag
     2281 cacgcagatc gtcgtccccg tcatgttctg ccacagctcg agcagcagat ccaggtcctt
     2341 catcgcgccc tgccccgaaa ggatgcgctc gaggatcttc acggtccacg cggtcccctc
     2401 gcggcactgg gtgcactgcg cgcacgattc gtgggcgtag aatttcgcca gccgcatgat
     2461 ctggtagacc atgtcggccg agtcgtcgaa cacgatcacg ccgcccgagc cgagcatcgt
     2521 gcccttctcg acgcagccct cgtagtcgag cagcgtgctc tcggtttcct cgcggttcag
     2581 gatcggcacc gacgacccgc cggggatgca cgccttgagc gtccgccccg ggcgcatccc
     2641 gccggccagc tcgtagagca ggtccttgag cgggaagccc atcgtcactt cgtagttgcc
     2701 cggtctggcg atgtggccgc acaccgagaa cagcttggtg ccggtgctct tggggttcga
     2761 gagcgacatc ttcttgtacc acgcggcgcc gtggcgcagg atgtggggca ccgccgccag
     2821 cgtttccacg ttgttgatcg tcgtcggcat gccgaacacg ccggcggccg cggggaacgg
     2881 cggcttgatg cgcgggttgc cgcgtttccc ctcgatcgag ttcatgagcg ccttctcttc
     2941 gccgcagatg taggcgccgg cgccgcgatg caggaacatc cggatgtcgc cgaacagctt
     3001 cgccgcgttc gcctcggcga tcgcctgctc caggatcgcc aggggctcgg tgaactcgcc
     3061 gcggacgtag atgtagaccg tcttcgcccg gatggcgtgc gccgcgatca ccgttccctc
     3121 gagcagggcg tgcggcgtcc agagcatgat ctcgcggtcc ttgaacgtgc ccggctccga
     3181 ctcgtcggcg ttcacgcaga gatagtgcgc cttcttcttc tccttcggca tgaacgacca
     3241 cttgaggccg gttgggaatc ccgcgccgcc gcgaccgcgc aaccccgagt tcttcacttc
     3301 ctcgatcacg g
//
LOCUS       DBNGZW010000448         1372 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1542199, whole genome shotgun sequence.
ACCESSION   DBNGZW010000448 DBNGZW010000000
VERSION     DBNGZW010000448.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1372)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1542199"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="AC16JC_10590"
     CDS             <1..264
                     /locus_tag="AC16JC_10590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165183.1"
                     /translation="ELDQAAAQVGGAASPQRDALAHDLETARRTAAQRLGTTVSAMEN
                     LRLDLLRLRAGIGSTDGLTEDLEAVRELAARVDAETELEADTK"
     gene            complement(320..1243)
                     /locus_tag="AC16JC_10595"
     CDS             complement(320..1243)
                     /locus_tag="AC16JC_10595"
                     /inference="COORDINATES: protein motif:HMM:NF021492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5165184.1"
                     /translation="MITTLRFTPTWLAAGLFVLAGCGDKTPKQASAKEPGAPEPTVSV
                     ASESTPAPAEPVSVNTAPVSFETAEAAFRERRYSDAVRDFTTYTADRPGNAWGFYMLG
                     LSSWKAGDFTTAEEALRQALSIDPTHVKSYLNLSRVLLDAGQPDSAIAMLDTVLKIDD
                     SLGEAYRLIGRAHDAKGERDEAVTAYHEALVLDSTDVWAMNNLGHSLIVQGSYDEALR
                     PLARAVELVPEVATFQNNLGIALERTGHFTLAAEAYRAALAADSGFTKASVNLQRVET
                     LKEDPNIPPVDLKSLAQEFVQQIGSWANAGE"
BASE COUNT          221 a          486 c          453 g          212 t
ORIGIN      
        1 gagctggatc aggcggcggc ccaggtgggc ggcgccgcat ctccgcagcg cgacgccctg
       61 gcccacgatc tcgagaccgc gcgccgcacc gcggcgcagc gcctcggcac gaccgtgagc
      121 gccatggaaa acctgcggct cgacttgctg cgcctgcggg cggggatcgg ctcgaccgat
      181 ggactcaccg aagatctcga ggcagtgcgc gaactcgccg cccgggtgga cgcggaaacc
      241 gagctcgaag ccgacaccaa gtgagacggg cggccccgcg ctgccgggac cgcccgtcga
      301 aggcaacgcc cgcgtccgct tactcgccgg cgttcgccca cgaaccgatc tgctgcacga
      361 actcctgtgc cagcgacttc aggtccaccg gcgggatgtt cggatcctct ttgagcgttt
      421 cgacccgctg cagattcacc gacgccttgg tgaaccccga atccgcggcc agcgccgccc
      481 ggtacgcctc ggccgccagc gtgaaatgcc ccgtccgctc cagcgcgatg ccgaggttgt
      541 tctggaacgt cgccacctcg gggaccaact cgactgcacg ggccagcggg cgcagcgcct
      601 cgtcatagga gccctgcacg atgaggctat gcccgaggtt gttcatcgcc cacacgtcgg
      661 tcgaatcgag caccagcgct tcgtgatagg cggtcaccgc ctcgtcgcgc tcgcccttcg
      721 catcgtgcgc gcggccgatc agccggtagg cttcacccag actgtcgtcg atcttcagca
      781 cggtatcgag catcgcgatg gcggaatccg gctgccccgc atcgagcagc acccggctca
      841 ggttgaggta gctcttcacg tgcgtcggat cgatcgacag cgcctgccgc agcgcttcct
      901 cggccgtcgt gaaatccccg gccttccagg acgacagacc cagcatgtag aagccccacg
      961 cgtttccggg acggtcagcg gtatacgtcg tgaaatcacg caccgcgtcg ctgtagcgcc
     1021 gctcccggaa ggccgcctcg gcggtttcga acgacacggg ggccgtgttc accgataccg
     1081 gctcggccgg cgcgggcgtc gattccgatg ccacgctcac cgtgggctcc ggagcgcccg
     1141 gctccttcgc gctcgcctgc ttgggagtct tgtcgccgca gccggccaac acgaacaatc
     1201 cggccgcgag ccaggtcggg gtgaaccgca gggtagtgat catcgattcc tcctccatca
     1261 ggggcgccgc gcgccggttg tggtttgcca gcgccctgtg gtaaagcagg agggggacca
     1321 ggcgggggag gcccaaggca tcgatgcaag cgattgacgg acggacagtt gg
//
LOCUS       DBNGZW010000449         3565 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_314499, whole genome shotgun sequence.
ACCESSION   DBNGZW010000449 DBNGZW010000000
VERSION     DBNGZW010000449.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3565
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_314499"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..373)
                     /locus_tag="AC16JC_10600"
     CDS             complement(<1..373)
                     /locus_tag="AC16JC_10600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165185.1"
                     /translation="MTGRRARLAALAGTVLLLGCAGAEQPEGKPAASTLVVGIDVSGS
                     FRDARLYDDAIDFAAHYIYAHLNGLGGLRTPTALFVASVGGARPGEPKPFHPINDFQG
                     RSVEQIAADLRAWFPSADAYTD"
     gene            complement(370..1602)
                     /locus_tag="AC16JC_10605"
     CDS             complement(370..1602)
                     /locus_tag="AC16JC_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165186.1"
                     /translation="MLASLQQSLPAQWQDTFRFLASGFTWILDFESAALGFVLSGPNP
                     AIVVVKCALILLPCALLVAALWCTMASLYTLPFRSGRGGFLTALLVSWWDAGRMTWFY
                     WAGLVRVGLVLLGWIWSLLKLAGSLVTRFIKFAFTSPFALLDWATRQYFQPGVPWIAF
                     LLILLWSALEATVFTFTLRPTLTEVLADLTGSELSGWAVTLILWPFLFILISGSFAAI
                     QALVEAIRSRRTSQIVSMALVEAFVMFFEVMFLYRELIDAITPWIAQQTGFQLGFFST
                     LALASFGWIGVRAMTWFLFGRFGTPALLGILARQNIAGPQRAGASVEFQTTEYWRGPV
                     AALKAEAEWFKKEARETFELLSLPVLQLLAAAVNFAVVVILSHPVFTLPFRNLEQVLA
                     TTPQFSAAARHPLPGATS"
     gene            2104..>3565
                     /locus_tag="AC16JC_10610"
     CDS             2104..>3565
                     /locus_tag="AC16JC_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5165187.1"
                     /translation="MFASLLLSTCSADHGTGPAPVAVRLAFQVSPGSSTAGTNFTPAV
                     KVVSLDSAGTNVASFADSITLTIDNNPGNGTLAGATKRAAVGGVATFADLSLNRPGRG
                     YRLRASSGNLTAATSASFDVAAGPATQLRFTAQPASTSGGAVMGSAIQVTALDALDNT
                     ADGFTGNVTLTITAGTGAAGATLSGTQTVAAVGGVATFTSLSIDKASTGYTLTATSSG
                     LIKATSASFDITIGPPTRLAFTTPPVTTTAGETITPAIVVTAQDAGGNPTPSFTADVT
                     VGIGTNPAAGTLSGTTTVPAVAGVATFATLSINRSGGGYTLTAAATGMTGAASAPFTI
                     APGATTELVFSVEPTNGTAGAAIAPAVQVTARDALGNTATGFSSDVTLAIEANPGGGT
                     LTGTTTVTASAGVAAFPTLSIDKTGSGYTVTATSAGLTSATSISFDIGPGTATRLVVT
                     AQPAGGTGGATITPAVQVTVQDALGNTAVGFAGEVTA"
BASE COUNT          641 a         1226 c         1249 g          449 t
ORIGIN      
        1 agtcggtgta ggcgtccgcg gacggaaacc aggcccgcag gtccgccgcg atctgctcga
       61 ccgagcgccc ctggaaatcg ttgatcgggt ggaacggttt gggctctccc ggccgcgcgc
      121 cgccgacgga ggccacgaag agggcggtgg gcgtgcgcag tccgccgagg ccgttcaggt
      181 gcgcgtagat gtagtgcgcc gcgaaatcga tcgcgtcgtc gtacagccgg gcgtcgcgga
      241 acgagccgct cacatcgatc cccaccacga gggtcgaggc ggcgggtttt ccctccggtt
      301 gttcggcccc ggcgcacccc aacagcagga ccgtcccggc cagcgcggcg aggcgcgcac
      361 ggcggccggt cacgaggtcg ctccgggcag gggatgacgt gccgcggcgg agaattgcgg
      421 cgtggtggcc aggacctgct ccagattccg gaacggcaac gtgaacaccg gatggctgag
      481 aatcaccacc accgcgaagt tcacggcggc ggccagcagc tgcagcaccg gcagggagag
      541 cagctcgaag gtctcccgcg cttccttctt gaaccactcg gcttcggcct tgagcgcggc
      601 caccgggccg cgccagtact cggtcgtctg gaactcgacg ctcgcccccg cccgctgcgg
      661 gccggcgatg ttctggcgcg ccaggatgcc gagcagcgcc ggcgtcccga agcggccgaa
      721 caggaaccag gtcatggcgc gcacgccgat ccagccgaag gacgccaggg cgagggtgga
      781 gaagaacccg agctggaacc ccgtctgctg ggcgatccag ggcgtgatcg catcgatcag
      841 ctcgcggtac aggaacatca cctcgaagaa catcacgaag gcctcgacca gggccatgga
      901 cacgatctgc gaggtccgcc ggctgcggat cgcctcgacg agcgcctgga tcgcggcgaa
      961 gctgcccgag atcagaatga acaggaacgg ccacaggatg agcgtcacgg cccagcccga
     1021 cagttcggag ccggtcaggt cggcgagcac ctcggtgagc gtcgggcgca gcgtgaacgt
     1081 gaacaccgtg gcttccagcg ccgaccagag gagaatgagg aggaaggcga tccaggggac
     1141 accgggctgg aagtactgcc gcgtcgccca gtcgagcagg gcgaagggac tggtgaacgc
     1201 gaacttgatg aagcgcgtga cgagcgagcc cgcgagcttc aagaggctcc agatccaccc
     1261 cagcagcacc aggcccacgc gcaccagccc ggcccagtag aaccaggtca tgcgccccgc
     1321 gtcccaccac gagacgagca gagcggtgag gaagccgccg cgcccggagc ggaacggcag
     1381 cgtgtacagc gaagccatgg tgcaccacag cgcggcgacg agcagcgcgc aggggagcag
     1441 gatgagggcg cacttcacga ccacgatcgc cgggttgggc ccggagagga cgaatcccag
     1501 cgccgcgctc tcgaagtcga gaatccaggt gaagccggac gcgaggaagc gaaacgtgtc
     1561 ctgccattgc gccggtaacg actgctgcaa ggatgcaagc atccgctcac ctccagagga
     1621 atccgccggt gacagagaag aaggaagaga ggtcgtgcgg gacgggcgct catccaatga
     1681 aggccgcacg atagcgccgc acctcggcaa cgcacgtgac ggcagtcacg cccgcggcgg
     1741 gcgtttggca agtggccgtc cctgcggcga ctgcggacgg tgctaccgga ggacggacag
     1801 cgcttccgac gccgggggag cgctcggccg actgccgggc gcagcggctc ttcgagcgct
     1861 acggcgccac agcctgcgtg ctacataacc gttgccgggt cgcgacgatg cgcccgcgag
     1921 cggctggtgg caggtcgctt cttccaagga aagcgtcgcg gtcgtcgaag caaggtgagc
     1981 ggcacaagaa atgccagcga ttggcttgac cccaacctct cgcgacctgt aaggcgtcac
     2041 accgtgaaca acgcccgcgc cttacgtacg attgcccgcc gcgtgaccca atgcggcggt
     2101 ttggtgttcg cctccctgct gctctccacg tgctcggccg accatgggac ggggcccgcg
     2161 ccggtggcgg tccggctcgc cttccaggtg agtccgggat cgtcaacggc cgggacgaac
     2221 ttcactcccg ccgtgaaggt cgtctcgctc gattcggcgg ggacgaacgt ggcgagcttc
     2281 gccgacagca tcacgctgac gatcgacaac aaccccggga atggcacgct ggccggcgcc
     2341 acgaaacggg ccgccgtcgg cggcgtggcg acgttcgccg atctgagcct caaccgaccc
     2401 ggcaggggct accgattgag agccagctcc ggcaatctga cggccgcgac gagcgcgtcg
     2461 ttcgacgtcg ccgcgggccc ggcaacgcag ctcagattca cggcgcagcc cgcctcgacc
     2521 tcgggcggag ccgtgatggg ctccgcgatc caggtcaccg ccctggacgc cctggacaac
     2581 acggccgacg gcttcaccgg caacgtcacg ctgacgatta cggcaggcac gggggccgcc
     2641 ggcgccacgc tctcgggaac gcaaacggtg gccgccgtcg gaggcgtcgc aaccttcacc
     2701 tccctcagta tcgacaaggc ctcgaccggc tatacgctga ccgccacctc gagcggcctc
     2761 atcaaggcga ccagcgcgtc cttcgacatc accatcgggc cacccacgcg cctggcgttt
     2821 accactccgc cggtcaccac cacggccggt gagacgatca caccggcgat cgtggtgacc
     2881 gcgcaggacg ccgggggcaa tccgacgccg agcttcaccg ccgatgtgac ggtgggcatc
     2941 gggacgaacc ccgcggcagg gacgctgtcg ggcaccacga ctgtgccggc cgtcgccggg
     3001 gtggcgacgt tcgccacgct cagcatcaac aggagcggtg gcggttacac gctcaccgcg
     3061 gcggcgaccg ggatgacggg agccgcgagc gctccgttca ccatcgcccc gggggcgacg
     3121 accgagctgg tcttcagcgt cgagccgacg aacgggaccg ccggcgcggc gatcgctccc
     3181 gcggtgcagg tgaccgcccg ggatgccttg ggcaacacgg cgacgggctt cagcagcgat
     3241 gtcacgctgg cgattgaggc gaatccgggc ggcggcacgc tgaccggtac gaccaccgtg
     3301 acggccagtg ccggtgtggc ggcgttcccg accctcagca tcgacaagac cggcagcggc
     3361 tacaccgtca ccgccacgtc cgccggcctg acgtcggcca cgagtatctc cttcgacatc
     3421 ggcccgggaa ccgcgacgcg gctcgtcgtc accgcgcagc ccgcgggcgg gaccggaggg
     3481 gcgaccatca ctcccgcggt tcaggtgacc gtccaggacg ccctggggaa cacggcggtc
     3541 ggcttcgcgg gtgaggtgac cgcgg
//
LOCUS       DBNGZW010000450         4437 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_314629, whole genome shotgun sequence.
ACCESSION   DBNGZW010000450 DBNGZW010000000
VERSION     DBNGZW010000450.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4437)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4437)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4437
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_314629"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1176)
                     /gene="carA"
                     /locus_tag="AC16JC_10615"
     CDS             complement(97..1176)
                     /gene="carA"
                     /locus_tag="AC16JC_10615"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006541 - L-glutamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HIR5165188.1"
                     /translation="MLLEDGAWFAGTAPLPFEPACAEVVFTTNLSGYQEVFTDPSYLG
                     QLVVMTAPMIGNYGINPEDVESDRPRVSGVVVRELSGHPSNWRSTGSLLDWLSAARIP
                     IIADVDTRQLTRHIRSKGAMRGVLATGAAPSEDVRARLMASPRMDGLDLASAATVPDE
                     YDSGPADAKHHVVAFDYGMKRNILRLFARNGCRVTVVPADTSSAWVRELAPSGVFLSN
                     GPGDPAAVGYAVETIRELADGSLPIFGICLGHQLLGLALGGETVKLPYGHRGGNHPVR
                     DLKTGQVLITSQNHGFAVRGDTGGVPGAKALEVTHLNLNDGTVEGVRHREYPIFAVQY
                     HPEASPGPHDAQEHFREFLQALDSQ"
     gene            complement(1193..1555)
                     /gene="secG"
                     /locus_tag="AC16JC_10620"
     CDS             complement(1193..1555)
                     /gene="secG"
                     /locus_tag="AC16JC_10620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015136 - sialic acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015739 - sialic acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HIR5165189.1"
                     /translation="MYTVLLILLLLDAVVMATAILLQAGQGGGLASLGGGSGTEMVMG
                     GRQATTLLHTVTWWCSGIFLFLALVLAVMSSRSSAPRSILAGGAAPAPVQQAPLPIQS
                     TPATPPATTPQQPTPPNR"
     gene            complement(1572..2321)
                     /gene="tpiA"
                     /locus_tag="AC16JC_10625"
     CDS             complement(1572..2321)
                     /gene="tpiA"
                     /locus_tag="AC16JC_10625"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HIR5165190.1"
                     /translation="MRRLLFAANWKMHLGPADAQSYMDVFLRAYQPAPDREVWFFPPA
                     VSLATVAARVIGRNDLLAGAQDVHWEPKGAFTGAISVPLAAQAGARAALIGHSERRHV
                     FGESDAETARKVRAVQDGGLVPVLCVGEKLAEREGGATLQVVQRQLAAGLEGQDGARL
                     DRIVIAYEPVWAIGTGRNATPQDAAAVHREIRRWLTQRGAKVARILYGGSVNLKNIAE
                     LVAEPELDGVLVGGASLDPAGWTQLVSVAAS"
     gene            complement(2318..3499)
                     /locus_tag="AC16JC_10630"
     CDS             complement(2318..3499)
                     /locus_tag="AC16JC_10630"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HIR5165191.1"
                     /translation="MTTRGLESLPVAALEGQRALVRVDFNVPLKNGAVSDDARIRAAA
                     PTIRYLRERGARVVLLSHLGRPKGGPDPKYSLRQIVRDVERVLGVTVEFIEDLATGAA
                     VSRRLPRGGVALVENTRFWPGEEANDPELAQQFAALGDFYVNDAFGSAHRAHASTEAV
                     AHLLKPAVAGFLMEKELRYLGAALEQPRRPFVAVLGGAKISGKIDVIEALLPKCDAIL
                     IGGAMACTFFASLGLEVGGSLVERDRTELARTLLAKGGQKLVLPRGATVAPSLERGAE
                     RRQVKSDEIPADMAVFDIDQATADDFRARILAARTVIWNGPMGVFETPPFDAGTRAVA
                     QAMVEATAKGATTVVGGGDSAAAVAGLEDKLSHVSTGGGASLEFLEGKPLPGVTALDA
                     A"
     gene            complement(3496..>4437)
                     /gene="gap"
                     /locus_tag="AC16JC_10635"
     CDS             complement(3496..>4437)
                     /gene="gap"
                     /locus_tag="AC16JC_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013506838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR5165192.1"
                     /translation="SKQTSLEFVGVNDITDPKTLGHLLKYDSVHGRFPGTVEVRGEAL
                     IVNGKEVRVTAIKEPEKLPWKELGVDLVLESTGRFTDRDSAAKHLAAGARKVVISAPA
                     KGEDITIVMGVNEKKYDPAAHHVISNASCTTNCLVPVVRVVLDNWGLVHGFMTTVHSY
                     TNDQNILDLPHKDLRRARAAALSIIPTTTGAAKATSLVIPEVKGKLDGVSLRVPTPDV
                     SIVDLTAEVKKSTTIDEVNAAFKQAAQGDLKGILDVSDEPLVSTDYIGSLYSSVVDAR
                     STNVIDGTLVHVSSWYDNEMGYSARCVDLLAYIGSRR"
BASE COUNT          690 a         1545 c         1512 g          690 t
ORIGIN      
        1 tgctgtgacc ttctcgacca gatcggcctt cgtcatccgt accccccgaa gggcgcaggg
       61 aataccagta accgggcgcc caacatagga gcttacctat tgactgtcaa gcgcttggag
      121 gaactcccgg aagtgctcct gggcgtcatg ggggcccgga gaggcctccg gatggtactg
      181 cacagcgaag atggggtact cccggtgcct gactccttct acagtgccat cattgaggtt
      241 aaggtgcgtg acctcaagtg ctttcgcacc tggaacgcct cccgtatcgc cccggacggc
      301 gaagccgtgg ttctgggaag tgataagcac ttgacccgtc ttgagatcgc gaaccgggtg
      361 gttaccaccc cggtgcccgt acgggagctt cacggtctcg cctcccaggg cgaggcccag
      421 cagttggtgg ccgaggcaga tcccgaagat ggggagggac ccgtcggcca gctcccggat
      481 cgtctcgacc gcgtagccga cggcggccgg atcgcccggg ccgttggaca ggaacacgcc
      541 cgacggcgcg agctcgcgca cccacgcgct ggatgtgtcg gccgggacca ccgtcacgcg
      601 acacccgttc cgtgcgaaca gccgcaggat gttgcgcttc atcccgtaat cgaacgcgac
      661 gacgtggtgc ttggcatccg ccggacctga gtcgtattcg tccggtacgg tcgcggcgct
      721 ggcgagatcg agtccatcca tccgcggcga ggccatgagc cgggcccgaa cgtcctcgga
      781 cggggcagcg cccgtcgcca gcacgccgcg cattgctccc ttggagcgga tgtggcgcgt
      841 gagctggcgc gtgtccacgt cggcgatgat cgggatgcgc gccgccgaaa gccagtcgag
      901 caggctgccg gtggagcgcc agttggaggg gtggcccgac agctcgcgca ccacgacgcc
      961 cgacaccctc ggccggtcgg actccacgtc ctcggggttg atgccgtagt tgccgatcat
     1021 cggtgcggtc atcaccacca gctgcccgag ataggaggga tcggtgaaga cctcctggta
     1081 cccgctcaga ttggtggtga agacgacctc ggcacaggcg ggctcgaacg ggagcggcgc
     1141 ggttccggcg aaccaggcgc catcctcgag gagcacgtag gccgggagac ggtcagcgat
     1201 tgggcggtgt cggttgctga ggggtggtcg ccggcggtgt cgcgggcgtg gactggatcg
     1261 gcaacggcgc ctgctgcacc ggcgcgggcg ccgctccgcc tgcgaggatg gaccgcggcg
     1321 cgctcgagcg cgacgacatc acggccagca cgagggcgag gaacaggaag atgcccgagc
     1381 accaccaggt gacggtgtgc agtaacgtcg tggcctggcg ccctcccatg accatttcgg
     1441 tcccggagcc gccacccagc gacgccagcc cgccgccctg ccccgcctgc agcaagatcg
     1501 cggtggccat gacgacggcg tcgagcaaca gcaggatcag cagaacggtg tacatgccgg
     1561 gcggagtata gctaggacgc ggcgaccgac acaagttgcg tccagcccgc gggatcgagc
     1621 gacgccccgc cgaccagcac cccatccagc tccggctccg ccacgagctc ggcgatattc
     1681 ttgaggttca cgctgccgcc gtacagaatc cgcgcgactt tcgccccgcg ctgggtgagc
     1741 cagcgccgga tctcccggtg cacggcggcg gcatcttgcg gcgtggcgtt gcgaccggtg
     1801 ccgatcgccc agacgggctc gtaggcgatc acgatccggt cgagacgggc cccgtcctgt
     1861 ccctcgagcc ccgccgcgag ctggcgttgc acgacctgca gggtcgcccc accctcccgc
     1921 tcggccagct tctcgcccac gcacaggacc gggaccagcc ccccgtcctg caccgcgcgc
     1981 accttccggg cggtctcggc gtcggactcg ccgaacacgt gccggcgctc ggagtggccg
     2041 atcagcgcgg cccgggcccc cgcctgtgcc gcgagaggga ccgagatggc tccggtgaac
     2101 gcccccttcg gctcccaatg gacgtcctgt gccccggcca ggaggtcgtt tcgcccgatc
     2161 acccgggcgg cgaccgtcgc gagcgagacg gcgggcggaa agaaccagac ctcgcggtcg
     2221 ggcgcgggct gataggcgcg gagaaagacg tccatgtagg actgcgcatc ggccggtccc
     2281 agatgcatct tccagttggc ggcgaacagc aggcgcctca tgcggcgtcc agtgcggtca
     2341 cgcccgggag cggctttcct tccaggaact cgagcgacgc gccacccccc gtggaaacgt
     2401 gggagagctt gtcctcgagc ccggcgaccg cggccgcgga atcaccgcca ccgaccaccg
     2461 tggttgcgcc cttggcggtc gcctccacca tcgcctgggc gacggcgcgc gtgccggcgt
     2521 cgaagggcgg ggtctcgaac actcccatcg ggccgttcca gatgaccgtc ctggccgcga
     2581 ggatccgggc ccggaagtcg tcggccgtgg cttgatcgat gtcgaaaacg gccatgtccg
     2641 ccgggatctc gtcgctcttc acctgccgcc gctcggcgcc gcgctcgagc gagggcgcta
     2701 ccgtggcgcc gcgcggcagc accagcttct ggcctccctt ggcgagcagg gtccgggcga
     2761 gctcggtgcg gtcccgctcc accagcgagc cgccgacctc gaggccgagg cttgcgaaga
     2821 aggtgcaggc catggcgccg ccgatcagga tggcgtcgca cttgggaagc agcgcctcga
     2881 tcacgtcgat cttgccgctg atcttggcgc cccccagtac cgcgacgaac ggccggcgcg
     2941 gctgctccag cgccgcaccg aggtagcgca gctccttttc catcaggaat ccggcgaccg
     3001 cgggcttgag cagatgcgcc accgcctccg tggaggcgtg ggcgcggtgg gccgatccga
     3061 acgcgtcgtt cacgtagaag tcgcccagcg cggcgaactg ttgcgccagc tccggatcgt
     3121 tcgcttcctc ccccggccag aagcgggtgt tctcgaccag cgccaccccg ccgcgcggca
     3181 gacgccggct gacggctgcc ccggtcgcca gatcctcgat gaactcgacc gtgaccccca
     3241 gcacgcgctc gacgtcgcgc acgatctgcc gcaacgagta cttgggatcg ggcccgccct
     3301 tgggccggcc caggtgggac aggagcacga cgcgcgcccc gcgctcgcgc aggtaccgga
     3361 tggtcggcgc ggcggcacgg atccgggcgt cgtcggaaac cgccccgttc ttcaagggaa
     3421 cgttgaaatc cacccggacc agcgcgcgct gcccttcgag cgcggcgacc ggaaggctct
     3481 cgaggccgcg cgtggtcacc ggcgcgatcc gatgtaggcg agcagatcca cgcagcgcgc
     3541 cgaatacccc atctcgttgt cgtaccagga ggagacgtgc accagcgtcc cgtcgatgac
     3601 gttggtggac ctggcatcca ccacgctgga gtacagactg ccgatgtagt cggtcgagac
     3661 gaggggctcg tcggagacgt cgagaatccc cttcagatcg ccctgggccg cctgcttgaa
     3721 ggccgcgttc acctcgtcga tcgtcgtgga cttcttcacc tcggcggtca ggtccacgat
     3781 cgagacgtcc ggggtgggca cccgcagcga gacgccgtcg agctttccct tgacctccgg
     3841 gatcacgagg ctcgtggcct tggccgcccc ggtcgtggtc ggaatgatcg acagcgcggc
     3901 ggcgcgggcc cggcgcagat ccttgtgcgg cagatcgagg atgttctgat cgttggtgta
     3961 gctgtgcacg gtcgtcatga agccgtgcac cagcccccag ttgtcgagca cgactcgcac
     4021 cacggggacg aggcaattgg tggtgcacga ggcgttggag atgacgtgat gggcggccgg
     4081 atcgtacttc ttctcgttca cccccatcac gatcgtgatg tcctcgccct tggcgggggc
     4141 ggaaatcacc acttttctgg cgccggcggc gagatgtttc gcggccgagt cgcgatcggt
     4201 gaaccggccg gtgctctcga gcaccagatc cacccccagc tccttccagg ggagcttctc
     4261 cggctccttg atcgccgtga cccggacctc cttcccgttc acgatcagcg cctcaccgcg
     4321 cacctcgacc gtccccggga accggccgtg caccgagtcg tacttgagca gatgcccgag
     4381 cgtcttgggg tcggtgatgt cgttgacccc cacgaactcc aggctggtct gtttgga
//
LOCUS       DBNGZW010000451         8771 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_587195, whole genome shotgun sequence.
ACCESSION   DBNGZW010000451 DBNGZW010000000
VERSION     DBNGZW010000451.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8771)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8771)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8771
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_587195"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="AC16JC_10640"
     CDS             <1..457
                     /locus_tag="AC16JC_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR5165193.1"
                     /translation="DGDGVADNLDRCPGTPPGSPVDQVGCVVLFREERAPGARPTLIL
                     RGVNFETGRSALTRESFAVLDQVAGSLAANPDINIEIAGYTDNTGSAAVNTRLSRARA
                     IAVRAYLARKGIAPSRMVARGFGPSNPVASNATAAGRAQNRRVELHKLP"
     gene            561..2867
                     /locus_tag="AC16JC_10645"
     CDS             561..2867
                     /locus_tag="AC16JC_10645"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR5165194.1"
                     /translation="MSRRASALLALILVGAPVAGQSRVAIPTPRSVLGFNPGDDRRLA
                     DWPTLVRYYQALARASDRVDYRELGKTTLGAPFVVLAISSPQNLRRLDVYRRMNDRLF
                     DPRTLRSSREALTAVHDGRTMVLITGGIHSDEVGGNLTPAVLAYRLATDTTARIRDIL
                     ENVILWLVPSLNPDGVTIVAKWYGRTLGTAAEGSDPPELYHHYIGHDNNRDWYAFTQI
                     ETQLVVDSLYNVWHPQLVLDLHQQGSNGSRMFLPPYLDPVEPNVDPLIVDGTNALGMA
                     IAWALAGEGRTGISVNAIYDAWTPARAYAHYHGAPRILSEAASADLATPVQVPFDRLE
                     ANARGFNPHERSWNFTAPWPGGRWALRDIVGYQTDVSLALLDHAARYRDHWLENFLTI
                     EWHAARGWRGWPYAFVIPRQQDTIALATLLGILVRGDVEIRVAQQAFSVGNQRLQSGS
                     YVVVLRQPAAAFAKTLLERQRYPDRRLYPGGPPERPYDVTAHTLPLLLGLTVLPVNDT
                     LRVPLSAPVGPPRASLGYPGFNTGAPRIGLYRSYVGAIDEGWTRWVFDTWRIPYIPVV
                     DSSFRGGRLKDKLDVLVLPDQPPRDIAEGLPQRRYPAPYPGGLGPDGIQALRQFVADG
                     GTLVALNEASRFAIQALLLPVRNVLEGLPDEDFYAPGSIFRVRLDPAHPIARDLPAET
                     AVWFEGGPAFDVLDSGQVRVVARYPDDPADVLLSGWVRHPERVAGRAALVDVKVGSGR
                     VILFGFRPQYRGQSLATYPLLFNSLQLH"
     gene            2927..4165
                     /locus_tag="AC16JC_10650"
     CDS             2927..4165
                     /locus_tag="AC16JC_10650"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HIR5165195.1"
                     /translation="MRQCVLAGALLTAAAIAPDRGLFAQSPQRLLAEAAAPSQACVAA
                     DASRVALLPAQARFTRRREFQWTRPGAVKALYVNAWAFGSSRLWSLVRLADSSAINAL
                     VVDVKDDTGCLLYRSQVRTAREIGADRCIRARDVEARLDTLHAHGIYPIARIVVAKDP
                     MLARGKPAWSVQSRSGGLWYDRIGSAWVDAYNDSVWVYSGLLAQEAVRLGFAEVQFDY
                     VRFPDEPRARMATAIFAARRGEETQRAAVRRHIQLLADYVRPMGVPVTFDVFGLAASN
                     TTTDLGIGQVWEDFVGVADVVLPMVYPSHYTRGAFGFRSPNANPYQIVRLALTDALAR
                     TRFPSTTLVRPYLQAFTLGRRLPRYTPTEIRDQIRAAQELGLESWVLWNPRSVYQRAA
                     LPPYPSVAGRVAASGATGGE"
     gene            4205..4897
                     /locus_tag="AC16JC_10655"
     CDS             4205..4897
                     /locus_tag="AC16JC_10655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165196.1"
                     /translation="MKRAVTSLAAVGLLACSVDSGTAPTTNVISTAEKAAVQQALASS
                     FGGDSLFASLSTFVLPFIAQATPLVNSPGDTTKLAAFQLQVSGTTLSAGLSGVLAWRG
                     FRSTTQTVDSVFLLIGGGFVPPLDDSLSADGAIDIGGSGTAWVVAQAADSSVQTWRAR
                     TGALRVSSVSYGEGSSVVQQGLTWTRFRGALTGNAHVTAKLVPDSTTSVSAGWDFSGG
                     LEAMMLKISPAP"
     gene            complement(4919..6946)
                     /locus_tag="AC16JC_10660"
     CDS             complement(4919..6946)
                     /locus_tag="AC16JC_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007179003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR5165197.1"
                     /translation="MISVLKHRPVSPLPDRVRLQGRVLFLTEDPQLVRRQLEGQDIDW
                     TPSMKLRDNISTDEITPAYICYYYDETLGDFPYLGLKCGDEFPVTRGSVRRGGFVASV
                     SGKRRGKGSSREQSPYAEMCAGIKLVVAENVERIYRENCQNLGILVTTDFSIIDRVWR
                     GEELPLAAFTAGEGEITRGIVEYGGLFNYNVARLQGRVRVAPPATTSRPMTSGEKILA
                     RHWIVDLSTGRVGVPAVQPGDEGFVATDIRFSHEYVTPMAAIFFEQRVGPEEKISDPG
                     SVLFFRDHLTFLDEAMTPERIKEGLLDVALELEKKQRAFAEKQGVRLYGELTRLGLHG
                     SEAICHSKILESHALPGQVIIGSDSHTPHAGAVGAVAFGVGTTAIFNSWITRDVRVTV
                     PETVRVVVRGRKPDNVTAKDFMLEILRHPYVKSGQAIGKLVEYCGPAVEALSVDERAT
                     MTNMTAEVGGFTGYVAPDERTAAYLREYRGMSRAEAERCCADLASDPGAVYCETIEID
                     AAGLRPMVALPGDPGNGLYIDELPGRVKVEIAYAGSCTAGKKEDMDMYARVLQEALDR
                     GERVAPGVRFFIQCGSQDVKRYCEERGYLEVFRRVGAQFLEPSCGACINAGPGVSRGK
                     DEVTISAINRNFPGRSGPGQLYLANPYTVAASAIAGHITEWEPGLTPVLAS"
     gene            7001..7867
                     /locus_tag="AC16JC_10665"
     CDS             7001..7867
                     /locus_tag="AC16JC_10665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165198.1"
                     /translation="MLLSLLLWQTNTTTPVIELTGRFQSPRITESSGVAVSRAHRGTL
                     WTHNDSGDGPYLFATDLRGADRGAIRVSGARAVDWEDIALGPCPLTVWQGACLYIGDT
                     GDNLERRRSVTVYAVPEPDPPTSPADTQRVTAAALALNLRYPDGSHDVEAMYVSPRDT
                     AVYLISKGRGGTIRLYRAGRDAWQSPRPVTVHLVQTLPIVPKARAGRRVTDAAMRPDG
                     ALVAVRTYSEIYFFTPGPGGHLTPSGHPCALDRLARVGEGIAFLDDVRVVITSEGNPL
                     GPGTIHTVRCPR"
     gene            8129..>8771
                     /locus_tag="AC16JC_10670"
     CDS             8129..>8771
                     /locus_tag="AC16JC_10670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165199.1"
                     /translation="MAALRAAVLADKSAQAIARKQKDSGLWGGNLLGYGPSKVYGWKE
                     TGTVFQYRRLLELGWPADHHVFRLADRLLFRLLSRDEDPVLLVEFQRAAKTDPGLGVW
                     VRSLHREAAAAALAHAGLVDDPRLRGAAHKVASDISAFLRSGLLEKLFRKQSGKTILD
                     PAATPPTIFGIEMLAFMPAVQRERAGFIERLATFFSVPAPKRAFFILAGKKFFK"
BASE COUNT         1332 a         3136 c         2833 g         1470 t
ORIGIN      
        1 cgacggcgac ggcgtcgcgg acaacctcga ccgttgtccc gggaccccgc ccgggtcgcc
       61 cgtggaccag gtcggctgcg tcgtcctgtt ccgtgaagag cgcgcgcccg gcgcgcggcc
      121 caccctgatc ctcagaggtg tcaatttcga gacggggcgt tccgcgctca cgcgggaatc
      181 gttcgcggtg ctggatcagg tcgccgggtc cctcgcggcg aaccccgaca tcaacatcga
      241 gatcgccggc tataccgaca acaccggctc cgcggcggtg aacacgcgcc tttcccgggc
      301 ccgggcgatc gccgtccggg cgtatctcgc gcgcaagggc atcgcgccgt cccgcatggt
      361 cgccagggga ttcggccctt cgaaccccgt cgcgtcgaac gcgacggcgg ccggacgggc
      421 gcagaaccgg cgcgtggagt tgcacaagct gccctgaccg ctgccggtcg ccgcggcgcc
      481 acgacgcccc acggtctcga agggcgtcgt ggcgtcgtgg cgtcgatgtc cctacctttc
      541 ccactcccga actcaccact gtgtcgcgcc gcgcatccgc acttctcgcc ctgatcctcg
      601 tcggcgctcc ggtcgccggc cagagcagag tcgccatccc gactccccgc tccgtgctcg
      661 gtttcaaccc cggcgacgac cgcaggctgg ccgactggcc gaccctcgtc cgctactacc
      721 aggccctggc ccgcgccagc gaccgcgtgg actaccgcga gctcggcaag acgacgctcg
      781 gggccccgtt cgtcgtgctg gcgatctcgt ccccccagaa cctccgccga ctcgacgtct
      841 atcgccggat gaacgatcga ctcttcgacc cccggactct gcgctcgagc cgcgaagccc
      901 tcacggcggt gcacgacggc cggacgatgg tgctgatcac gggagggatc cactcggacg
      961 aggtaggcgg gaatctcacc cctgcggtgc tcgcctaccg gctggcgacg gacaccaccg
     1021 cgcggatccg tgacatcctc gagaacgtca tcctctggct ggtgccctcc ctcaacccgg
     1081 acggcgtgac gatcgtggcc aagtggtacg gcaggacgct cggcaccgcc gccgaaggaa
     1141 gcgacccgcc cgagctgtac caccactaca tcgggcacga caacaaccgg gactggtacg
     1201 ccttcacgca gatcgagacc cagttggtcg tcgattcgct ctacaacgtg tggcaccccc
     1261 agctggtgct cgatctgcac cagcagggtt ccaacggctc gagaatgttc ttgcccccgt
     1321 acctggaccc ggtcgagccg aacgtcgatc cgctgatcgt cgacgggacc aatgcgctgg
     1381 gcatggccat cgcctgggcg ctggccggcg aggggaggac cggcatctcg gtgaacgcca
     1441 tctacgatgc gtggacccca gcacgggcct acgcgcacta ccacggcgct ccccgcatcc
     1501 tgtccgaagc ggcgagcgcc gacctcgcga cccccgtgca ggtgccgttc gatcgtctgg
     1561 aagcgaacgc acgcggcttc aacccgcacg agcggtcctg gaatttcacc gccccatggc
     1621 cggggggccg ctgggccctg cgggacatcg tcggttacca gaccgacgta tcgctcgcgc
     1681 tgctcgacca cgcggcccgg taccgcgacc actggctcga gaatttcctc accatcgagt
     1741 ggcacgcggc ccgcggctgg cgcggctggc cctacgcgtt cgtaatcccc cgccagcagg
     1801 acacgatcgc tctcgcgacg ctgctcggca tcctggtccg tggcgacgtc gagatccggg
     1861 tcgcccagca ggcgttttcc gtgggcaacc agcggttgca gagcggcagt tacgtcgtcg
     1921 tgctgcggca gccggccgcc gcgttcgcca agacgctgct cgagcgacag cgctatccgg
     1981 atcgccgcct ctatcccggc gggccacccg agcgtccgta cgacgtcacc gcccacaccc
     2041 tcccactgct gctgggactc accgtgctgc cggtgaacga cacgctgcgg gtaccgctct
     2101 ccgcccccgt cggcccgccg cgcgcgtcgc tcgggtaccc gggcttcaat acgggcgctc
     2161 ctcgaatcgg actgtatcga tcctatgtcg gagcgatcga cgaagggtgg acccggtggg
     2221 tgttcgacac gtggagaatc ccctacatcc cggtggtgga ctccagcttc cgtggcggcc
     2281 gcctcaagga caagctggat gtcctcgtgc tcccggacca gccgccgcgt gacatcgccg
     2341 aagggctgcc acaacgccgc tacccggcgc cgtatccggg cggcctcggt cccgacggca
     2401 tccaggcgct gcggcagttc gtagcggacg gcggcaccct cgtggcactg aatgaagcca
     2461 gccggttcgc gatccaggcg ctgctcctgc cggtacgcaa cgtcctcgag ggactgccgg
     2521 acgaggattt ctacgccccc ggctcgatct tccgcgtgcg gctcgatccc gcacacccca
     2581 tcgcgcgcga cctgccggcc gagaccgccg tgtggttcga aggcggcccc gccttcgacg
     2641 tgctggactc ggggcaagtg cgggtcgtcg cgcgctaccc cgacgatcct gcggatgtcc
     2701 tgctgtcggg gtgggtgcgg cacccggagc gggtcgcggg tcgggcggca ctggtcgacg
     2761 tgaaggtggg gtccggccga gtcatcctct tcgggttccg accgcagtac cgcggtcaga
     2821 gtctcgcgac ttacccgtta ctgttcaaca gcttacaact gcactagttc tttgtgatct
     2881 agggctcttg ctggctttag gactattttc tatcgtctag ccccctatgc ggcaatgtgt
     2941 gcttgctggc gcgctcctca ctgcggccgc gatcgccccc gatcgcggcc tgtttgccca
     3001 gtccccacag cggttgctcg ctgaggccgc ggcgccctcg caggcctgcg tcgcggcgga
     3061 cgcgagtcgc gtggcgctgt tgcctgccca ggcgcggttc acccgccgtc gggagtttca
     3121 gtggacccga cccggcgccg tcaaagcatt gtatgtgaac gcctgggcct tcggctccag
     3181 tcgactttgg tccctggttc gtctggctga ttcgagcgcc atcaacgcct tggtcgtcga
     3241 cgtcaaggac gacaccggtt gcctgctgta ccgctcccag gtccgcacgg cccgggaaat
     3301 cggcgccgat cgttgcatcc gcgcccggga tgtggaagcg cgactggata ccctgcacgc
     3361 gcacgggatc taccccatcg cgcgcatcgt cgtggcgaag gacccgatgc tcgcccgagg
     3421 caagcccgcc tggtcggtgc agtcccgcag cggcgggctg tggtacgacc ggatcggcag
     3481 cgcctgggta gacgcttaca acgattcggt gtgggtgtat tccggcctgc tggcgcagga
     3541 ggcggtcagg ctcggctttg ccgaggtcca attcgattat gtgcgcttcc ccgatgagcc
     3601 ccgcgcacgg atggcgacgg cgatcttcgc cgcgcggcgc ggtgaggaga cgcagcgtgc
     3661 ggccgtccgg cgtcacatcc agctgctggc cgattacgtg cggcccatgg gcgtcccggt
     3721 caccttcgac gtgttcggcc ttgccgcatc gaacaccacc accgatctgg gcatcggcca
     3781 ggtgtgggag gacttcgtcg gcgtggcgga cgtggtgctt ccgatggtgt atcccagcca
     3841 ctatacccgc ggcgccttcg gcttccgcag cccgaacgcc aacccctacc agatcgtgcg
     3901 gctggcactg acggatgccc tggcgcgcac ccgtttcccg agcacgaccc tggtccggcc
     3961 ttatctgcag gccttcacgc tcggccgccg cttgccgcgc tacaccccca cggagatccg
     4021 tgaccagatc cgggccgccc aggagctggg cctcgaatcc tgggtgttgt ggaatccccg
     4081 cagcgtctac caaagagccg ccctgccgcc gtatccttct gtcgcgggac gcgtggccgc
     4141 ctccggagct accggcggcg aatagacccg cgtccggtct gtcccgacaa aaggagcccg
     4201 ttgtatgaag cgtgcggtca cctcgctcgc cgccgtgggc ctactcgcgt gttccgtcga
     4261 cagcggcacc gccccgacga cgaacgtcat ctcgaccgcg gagaaggccg cggtgcagca
     4321 ggcactcgcc tcctcgttcg gcggcgacag tctgttcgcc agcctctcga cgttcgtgct
     4381 gccgttcatc gctcaggcga cgccgctggt caacagtccc ggtgatacga ccaagctcgc
     4441 ggcgtttcag ctccaggtgt cgggcaccac cctgtcggcc ggtctgagcg gggtcctcgc
     4501 ttggcgcggc ttccgctcca cgacgcagac cgtggactcc gtgttcctgc tgatcggcgg
     4561 cggtttcgtg ccgccgctcg atgattcgct cagtgccgat ggcgcaatcg atatcggcgg
     4621 gtcgggcacg gcctgggtgg tggcgcaggc cgcggattcg agcgtgcaga cctggcgcgc
     4681 gcgcacgggc gccctgcggg tgagcagcgt cagctacggc gagggcagca gcgtcgtcca
     4741 gcaggggctc acctggaccc ggtttcgcgg cgccctgacc ggcaatgcgc acgtcacggc
     4801 gaagctcgtc cccgattcga cgacgagcgt gagcgcgggc tgggatttca gcgggggact
     4861 cgaagcgatg atgttgaaga tctcgccggc gccctgaggt ggggccggcg ggcgaccctc
     4921 aggacgccag gaccggcgtc aggccgggct cccattcggt gatgtgaccg gcgatggccg
     4981 aggccgcgac ggtgtagggg ttggcgagat acagctggcc cggccccgag cgaccgggga
     5041 aattgcgatt gatcgcgctg atggtcacct catccttgcc gcgggacacg cccgggcccg
     5101 cattgatgca ggcgccgcag gacggctcca ggaactgggc gccgacgcgc cggaacacct
     5161 cgaggtagcc ccgttcctcg cagtagcgct ttacgtcctg ggagccgcac tggatgaaga
     5221 accgcacgcc cggcgcgacg cgttctccgc gatccagtgc ctcctgcagc acgcgggcgt
     5281 acatatccat gtcttccttc ttcccggcgg tgcaggaccc ggcgtacgcg atttcgacct
     5341 tcacgcgtcc cggcagctcg tcgatgtaga ggccgttccc tggatcaccc ggaagagcca
     5401 ccatcggccg cagcccggcc gcatcgatct cgatcgtctc gcaatagacg gcgcccggat
     5461 cgctcgccag atcggcacaa cagcgctcgg cctcggcgcg ggacatacca cggtactccc
     5521 tcaggtacgc ggcggtccgc tcatccggag cgacgtagcc cgtgaacccg ccgacctcgg
     5581 cggtcatatt cgtcatggtg gcccgctcgt ccaccgacag tgcttcgacc gccgggccgc
     5641 agtactccac cagcttccca atcgcctggc cgctcttcac gtaggggtgg cgcaggatct
     5701 cgagcatgaa atccttggcg gtcacattgt ccggcttgcg cccgcgcacc acgacccgca
     5761 cggtctcggg gacggtgaca cgcacgtcgc gcgtgatcca cgaattgaag atcgccgtgg
     5821 tgccgacgcc gaacgcgacg gcgccgaccg ccccggcatg gggggtgtgg gaatcggagc
     5881 cgatgatcac ctgccccggc aacgcgtgag attccaggat cttggagtgg cagatggcct
     5941 cggagccgtg cagaccgagc cgcgtgagct ccccgtagag ccgcaccccc tgcttttcgg
     6001 cgaaggcgcg ctgcttcttc tcgagctcca acgccacgtc cagcagccct tccttgatgc
     6061 gctccggcgt catcgcctcg tcgaggaacg tcaggtggtc gcggaagaag agcaccgagc
     6121 ccggatcgct gatcttctcc tcggggccga cccgctgctc gaagaagatc gcggccatgg
     6181 gcgtcacgta ctcgtggctg aaccggatat cggtcgcgac gaacccttcg tcgccgggct
     6241 ggacagcggg cacgccgaca cggcccgtgg acaagtccac tatccaatgc cgtgccagga
     6301 tcttctcgcc cgacgtcatc ggccgtgacg ttgtggccgg aggcgcgacg cgcacccggc
     6361 cctgcagccg ggccacgttg tagttgaaca gcccgccgta ctcgacgatt ccccgcgtga
     6421 tctccccttc accggcggtg aacgccgcca ggggaagctc ctcaccgcgc cacacccggt
     6481 cgatgatcga aaagtcggtc gtcacgagga tgcccagatt ctggcagttt tcgcggtaga
     6541 tgcgctcgac gttctccgcg accacgagct tgatgccggc gcacatctcg gcgtagggcg
     6601 actgctcacg cgaggagccc ttcccgcgac gcttcccgct caccgacgcg acgaacccgc
     6661 cacggcgcac cgagccccgt gtgaccggga actcatcccc gcacttgagg cccagatagg
     6721 ggaagtcccc gagcgtctcg tcgtagtagt agcagatgta ggccggggtg atctcgtcgg
     6781 tcgagatgtt gtcgcggagc ttcatactcg gcgtccaatc gatgtcctgg ccctcgagct
     6841 gtcggcgcac gagctgcgga tcctccgtga gaaagaggac ccgcccctgc agccgcaccc
     6901 gatcggggag cgggctgacc ggccgatgct tcaacaccga gatcatgatg agaagatcac
     6961 cccttgcccg gagacgatca agcgcattcc ttcaattccc atgctcttgt ccctgctgct
     7021 gtggcagacg aacacgacca ctccggtcat cgagttgacc ggcagattcc agagtccacg
     7081 catcacggaa agctcgggcg tcgccgtgag tcgcgcccat cggggcacgc tctggaccca
     7141 caacgattcc ggcgacgggc cctacctgtt tgcgaccgat ctccgcggtg ccgatcgcgg
     7201 agcgatccgc gtctccggcg cccgcgccgt ggattgggag gacatcgccc tggggccgtg
     7261 tcccctcacg gtctggcaag gcgcgtgcct ctacatcggc gacacggggg acaatctcga
     7321 gcgccgccgc agcgtcaccg tctatgccgt tccggaaccc gaccccccga cgagccccgc
     7381 cgacacacag cgagtcaccg ctgccgcgct tgccttgaac ctccgctacc cggacggatc
     7441 gcacgacgtc gaggccatgt acgtctcgcc acgcgacacg gcggtgtacc tcatctccaa
     7501 ggggcgcggg gggacgatcc ggctctaccg tgccggccgc gacgcgtggc aatccccacg
     7561 gccggtgacg gtgcatctcg tccagacgct gcccatagtc cccaaggccc gtgcaggtcg
     7621 ccgcgtgacc gacgccgcca tgcgccccga tggcgccctg gtggcggtcc gcacttacag
     7681 cgagatttac ttcttcaccc cggggccggg cgggcacctc accccgagcg gccatccgtg
     7741 cgcgctcgat cggctcgcgc gtgtcggcga agggatcgcc ttcctggacg atgtgagggt
     7801 ggtgatcacg agcgaaggca atccactcgg gccgggcacc atccacaccg tacgctgtcc
     7861 ccgctgagcg acagccttcg taagacaccg tccaacaata gcttagagct ttacggaagt
     7921 gggggctggc ctgtggcgca ctgctccggt aagtttgcgc cgcttttagc ccgttccagg
     7981 cgaccttccc aaaggcaccg agcgcaatct ggaacttccc gcgtcacacc cgtttcccct
     8041 gacctggctg tacgaggccg cgagcctgcc catccagtat cgcgccctta ccgaggtcgc
     8101 tcccccggca gcgcgcgatc cagagcgcgt ggccgcactg cgcgccgccg tgctcgccga
     8161 caagagcgcg caggccatcg cacgcaagca gaaggactcc gggctatggg gcgggaacct
     8221 gctgggatac ggaccgtcga aggtctatgg ctggaaggaa accggcacgg tcttccagta
     8281 ccgtcggctg ctcgagctgg gctggccggc ggaccaccac gtgttccggc tggccgaccg
     8341 cctcctgttc cgcctgctgt ctcgcgacga ggacccggtg ttgctggtcg agtttcagcg
     8401 ggcggccaag acggacccgg ggctgggcgt ctgggtgcgc agcctgcacc gggaagccgc
     8461 cgcggccgca ctggcccacg ccgggctcgt ggatgatcct cgcttgcgcg gggcggctca
     8521 caaggtcgcc tcggacatct cggccttcct tcgcagcggg ctcctcgaga agctgtttcg
     8581 caagcaatcg ggtaagacca tcctcgatcc ggcggcgaca ccgccaacca tcttcgggat
     8641 cgagatgctc gcgttcatgc ccgctgtcca gcgtgagcga gccgggttca tcgagcggct
     8701 ggcgacgttt ttcagcgtgc cggcacccaa gcgtgcgttc ttcattttgg ccgggaagaa
     8761 gttcttcaag c
//
LOCUS       DBNGZW010000452         2117 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_794229, whole genome shotgun sequence.
ACCESSION   DBNGZW010000452 DBNGZW010000000
VERSION     DBNGZW010000452.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2117)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2117)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2117
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_794229"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="AC16JC_10675"
     CDS             complement(<1..930)
                     /locus_tag="AC16JC_10675"
                     /inference="COORDINATES: protein motif:HMM:NF012621.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR5165200.1"
                     /translation="MARQRIILPIEGMSCAACAQTVQQALGAARGVASASVNYATAKA
                     AVEYDDAAVGVGQLAKAVRDAGYECGQATVTFGIPDLHYAPSTAGLERALAAVPGVIR
                     AAANQATETVTVVYVPGVTTAGQLEDAVVAAGFRVGEPIAAEDPVERERLARSREIRT
                     LTGQFALAASAALLTMLGAMLLMAGHDAATGIGRVDLLGRLLMPLAMRLRSAVGGGDP
                     TLLKLLLLGLTLPVLLWSGRRFYRGAWSGFRHRTADMNTLIAVGTGAAFLYSVVATLV
                     PGVFTRAGLPADVYYEAVCAIIALILLGRLLEAR"
     gene            complement(923..1351)
                     /locus_tag="AC16JC_10680"
     CDS             complement(923..1351)
                     /locus_tag="AC16JC_10680"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5165201.1"
                     /translation="MTAAQLIVTALGVAAIVWVLWYFLFSRSPAVRPGGERGGAVQEI
                     AVTVKGGYTPDTILVQAGKPVRLQFYRDETADCSERVVFEHFGIDRLLPPFQTTTIEF
                     TPDEAGEYPFRCGMSMLKGLLVVEPPGDRLPAPTPHGGHG"
     gene            complement(1359..>2117)
                     /locus_tag="AC16JC_10685"
     CDS             complement(1359..>2117)
                     /locus_tag="AC16JC_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HIR5165202.1"
                     /translation="AKPVLIGTAVVGATTMIFSIIVALTNGMQPELVTNLSILHPPFL
                     LGLIAGGAMIYWFTGASTQAVTTGAYRAVEFIKANIKLEGSTKASVADSKRVVAICTQ
                     YAQRGMFVIFLAVFFATLGFAFVEPYFFIGYLISIALFGLFQAIFMANAGGAWDNAKK
                     VVETVLKQKGTELHDATVVGDTVGDPFKDTSSVAMNPIIKFTTLFGLLAVELAVSLTR
                     ERGATLTHGFAAAFFILSVVFVWRSFYRMRIESK"
BASE COUNT          367 a          731 c          724 g          295 t
ORIGIN      
        1 gcgggcctcg agcagtcggc cgagcaggat gagcgcgatg atggcgcaga ccgcttcgta
       61 gtagacgtcg gcgggcaggc cggcgcgggt gaacacaccc ggcacgagcg tcgcgaccac
      121 cgagtagagg aacgcggcac cggtgccgac cgcgatcagc gtgttcatgt cggcggtccg
      181 gtgccggaac ccgctccagg cgcccctgta gaaccgccgc ccagaccaga gcagcaccgg
      241 gagggtcagt ccgaggagca gcaacttgag gagcgtcggg tccccccctc cgaccgcgct
      301 gcgcaaccgc atcgccagag gcatgagcag ccgacccagc aggtccaccc ggccgatgcc
      361 ggtcgcggca tcgtggcccg ccatcaacag catggcgccg agcatcgtga gcagcgcggc
      421 gctggcggcg agcgcgaact gcccggtgag cgtccggatc tcgcggctgc gcgccagccg
      481 ctcgcgctcc accgggtcct cggccgcgat cggctcgccc acccggaacc cggcggcgac
      541 cacggcgtcc tcgagctgcc ccgccgtcgt gacgcccggg acgtagacga ccgtcacggt
      601 ctccgtcgcc tgattggcgg cggcgcggat cacgccgggc acggcggcca acgcgcgctc
      661 gagcccagcg gtgctgggcg cgtaatggag gtcggggatg ccgaacgtga ccgtcgcctg
      721 tccgcactcg tacccggcgt cgcgcaccgc cttcgccagc tgccccacac cgaccgccgc
      781 gtcgtcgtac tctaccgccg ccttcgcggt ggcatagttg acgctcgccg aagccacgcc
      841 acgtgcggcg ccgagcgcct gctgcaccgt ctgggcgcag gcggcgcagc tcatgccttc
      901 gatgggcagg atgatccgct gtctagccat ggccgccgtg gggcgtgggc gcggggagtc
      961 gatcgcccgg cggctcgacc accagcagtc ccttcagcat ggacatcccg caccggaagg
     1021 gatattcgcc cgcctcgtcg ggcgtgaatt cgatggtggt ggtctggaag ggcgggagca
     1081 ggcggtcgat accgaagtgc tcgaacacca cccgctcgga gcaatcggcc gtctcgtcac
     1141 gatagaactg cagccgcacg ggcttcccgg cctgcacgag aatcgtgtcc ggagtgtacc
     1201 cgcccttcac cgtgacggcg atctcctgca ccgcaccgcc ccgctccccc ccggggcgca
     1261 cggcagggga gcgggagaag aggaaatacc acagcaccca gacgatggcg gcgaccccga
     1321 gcgcggtgac gatgagctgc gccgcggtca tgtccggctt acttgctttc gatgcgcatc
     1381 cgatagaagg accgccacac gaacaccacc gagagaatga agaacgcggc ggcgaacccg
     1441 tgcgtcaacg ttgcgccccg ctcgcgcgtg agcgacacgg cgagctccac cgccagcagg
     1501 ccgaacaggg tcgtgaactt gatgatgggg ttcatggcca ccgacgaggt gtccttgaac
     1561 gggtcgccca cggtatcgcc gaccaccgtc gcgtcgtgca gctcggtgcc tttctgcttg
     1621 agcacggtct cgacgacctt cttggcgttg tcccaggccc cgccggcatt ggccatgaag
     1681 atggcctgga acaggccgaa cagggcgatg gagatgaggt agccgatgaa gaagtacggc
     1741 tcgacgaacg cgaacccgag ggtcgcaaag aacaccgcca ggaagatcac gaacatgccc
     1801 cgctgggcgt actgcgtgca gatcgccacg acccgcttcg aatcggcgac ggacgccttg
     1861 gtgctgcctt ccagcttgat gttcgccttg atgaactcca ccgcccgata cgccccggtg
     1921 gtcaccgcct gggtggaggc gccggtgaac cagtagatca tggcaccgcc ggcgatcagg
     1981 cccagcagaa acgggggatg gaggatcgac aggttggtca cgagctcggg ctgcatgccg
     2041 ttggtgagag ccacgatgat cgagaagatc atcgtggtgg ccccgacgac ggccgtgccg
     2101 atcagcacgg gcttcgc
//
LOCUS       DBNGZW010000453         5945 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1473892, whole genome shotgun sequence.
ACCESSION   DBNGZW010000453 DBNGZW010000000
VERSION     DBNGZW010000453.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5945
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1473892"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1587)
                     /locus_tag="AC16JC_10690"
     CDS             complement(<1..1587)
                     /locus_tag="AC16JC_10690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HIR5165203.1"
                     /translation="MRRSLSVVLWSLVGLLAAFLGALSALVGTKAGHDLLTRVSTAAL
                     ESVVGGRVELGSTSGSLLTGLAYADVRLYDHDTTLVAWLPRAELDYNLLDFVAGQIVL
                     QRVRLEHPYFNIVQHKNGRLNIEELLHLGEPSKGPRRGPKPLVVLRNVTIVDGDLVLR
                     LQSRPTRGDSLLEIDAFGSDGRRRVRRFRHLTAQLTAFRISAPGQRGLRVDLARLAVG
                     ISDPSVEIANMRGRVTIDGDSLNADLPEVRLPASRFSVRGGLAWPHDTLLYDLIVGAD
                     SVTLSDTRFIDPRFPEGAVLHGALAVRSHGSRMLEVRLDTLDLVYNGGRAQGRATAFI
                     AADSGLVAVRSTDVTTTDLDLALPRAFLDTLPFYGHLSGRAVADGQLSDLTLDADWTF
                     RDSLVADWPVSRVRGRGTVNLTAPEGITFLPFTVDTAAIDFGTFAEFVPAPLHGTLDA
                     VGMLSGPLRNVRFDGALRHVDGDRPASVAHGHFGADARRDTVALDVDAQLDTLSFDGL
                     AGSFPGLGVRGAVAGAVRLAG"
     gene            complement(1590..3749)
                     /locus_tag="AC16JC_10695"
     CDS             complement(1590..3749)
                     /locus_tag="AC16JC_10695"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA];
                     GO_process: GO:0071709 - membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/OMP85 family outer membrane protein"
                     /protein_id="HIR5165204.1"
                     /translation="MRFLRRFALAVLLCVPAVPTAPIAAQQEPERVVRQLDFVGNHAI
                     DDYTLGTVIATSKSSWFARAALVRSLGLGAKRYFDELEFRRDVVRLILYYRQSGYMQA
                     VVDTSVRRTPRDVYITFRIFEGAPVRVRQLALEGVSGIVDERPLRRALPLKVGDPFNR
                     FLMQASADTMAAWLRNRGFPYAQVLRNFDAEAAELAADVRFEAVPGPYMRIGRVVVEG
                     LQRVDTATVLHALSVHTGAQYGEGLLYQSQRDLYGAGMFRAASVSLRDSVPPADGDST
                     VDVLVRVTEGSRQRVGLGAGYGTLDCFRAQGGWSSYGFLGDARVLDLSARLSKIGVGA
                     PLNLGFKHTQQSLCFPLYGDSTSDTLNYNVALTLLQPTFLSPRHSASIGVFAERRSEV
                     RTYTRIQVGANVGVTLNARRRVPLTFIYGLSRGRTDANDAAFCSVFNVCDDSTLRYLR
                     NPHRFASLTLTAGLRTEDFLLDPSRGGRVGITLLHSSRLLGSDTLYEFNRGELEVARY
                     MPFGRRGVFAWRVRAGALVPAGVTAAGQRVQFVPPEQRFYAGGPNSVRGYPANELGPV
                     VYVITDTTCADAAHCAYSVVGGDTVYNNATVSPIGGNAILLANAELRVPAPVFPDRLR
                     VAFFVDVGQVYQQQGDLFSLRALRVTPGVGVRVTTPLGPIRVDVAYNGYDRESGRLKL
                     LNRSAGTLTDYRLNYQPRRSGSLLNRLTLQFAIGQVF"
     gene            3824..5389
                     /locus_tag="AC16JC_10700"
     CDS             3824..5389
                     /locus_tag="AC16JC_10700"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5165205.1"
                     /translation="MPRFRAYPTALLTLLAACSGGRGRGAAGAPLVYYESYDPRSLDP
                     ALSTDVPTGEMVTLLYDGLTRFGADGSLLPALADRWSVDAAGLRYVFHLRPGVKFHDG
                     APLDPEAVRRSFLRVLDPKSTGGRAWPLLPIDGASAYAAGTTDRVRGIEVLGDTGVAL
                     TLTQPLAVFPKFLAMPVASIAGPDSGKDLSTRPNGTGAWRFASWAHDDALHFARNPAY
                     WGGAPQADSLVVRIIPDQLVRAAEFESGRLDVVEVPFGETARWRERHPAWLQQKPALR
                     VTYIALNTRRGPLRDARVRQAINHAVNVDQILATVWSGRGIRARGAIPPTLAGGDTTR
                     PAFAYDPALAKQLLAQAGHAGGFALQLWRTADNETLGRVAQAVQSDLAAVGIRVEIVT
                     RDAPSQREAARAGNADMALLDWWADYPDGDNFLYPLFASASFGPGGNYAFYSDPVTDS
                     LIRTARRTQDDAARTALYHAIDQRVYQAAPWLYLWFPVDLWAQSPSITGWELPVIFNG
                     QRWTAVRRASPQP"
     gene            5386..>5945
                     /locus_tag="AC16JC_10705"
     CDS             5386..>5945
                     /locus_tag="AC16JC_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5165206.1"
                     /translation="MTGVLGRRLLLVVPTLIGVLVAAFLLLEVAPGDPVQAMVGERAD
                     SATIARLRAELRLDDPLPLRFGHYVWGVVRGDLGRSYITRRPIAQDLLERFPKTAQLA
                     LTAMTFAALAGISLGVVAAIRPGGAADRAASLLAYLGVSFPVYWVALLMVLLFAVTLR
                     WFPPSGSGGLAFLVLPAATLGMRSVAV"
BASE COUNT          904 a         2152 c         2062 g          827 t
ORIGIN      
        1 accggcgagc cgcaccgcgc cggccacggc gccgcgcact cccagtcccg ggaagctgcc
       61 ggccaggccg tcgaacgaca gcgtatcgag ctgggcgtcc acgtcgaggg cgacggtgtc
      121 gcggcgcgcg tcggccccga agtggccgtg cgcgacactc gcgggacggt ctccgtccac
      181 gtgtcgcagc gcgccgtcga accgcacgtt ccggagcggc ccgctgagca tccccaccgc
      241 gtccagcgtg ccgtgcagcg gcgccggcac gaactcggcg aacgtgccga aatcgatcgc
      301 cgcggtgtcc accgtgaacg gcaggaaggt gatcccttca ggcgcggtga gattcaccgt
      361 tccccgcccc cgcacccggg acaccggcca gtccgccacg agcgagtcgc ggaaggtcca
      421 gtcggcgtcc agggtgaggt cggagagctg tccgtcggcg acggcccgtc cgctcaggtg
      481 cccgtagaac ggcagcgtgt cgaggaaggc ccggggaagc gcgaggtcca gatccgtggt
      541 cgtgacgtcg gtgctgcgca cggccacgag gcccgaatcg gcggcgatga acgccgtcgc
      601 ccggccctgg gcccggccgc cgttgtagac cagatcgagg gtgtcgagcc gcacctccag
      661 catccggctg ccgtgggagc ggacggccag ggcgccgtgc agcacggcgc cctccgggaa
      721 gcgcggatcg atgaagcggg tgtcggacag cgtgaccgaa tcggcgccga cgatcagatc
      781 gtacaacagc gtatcgtgcg gccaggcgag accgccccgc acggaaaagc gtgaagcggg
      841 gagacgcacc tccgggaggt cggcgttcag ggaatcgccg tcgatggtga cccggccgcg
      901 catgttggcg atctcgaccg agggatcgct gatgccgacg gcgagacgag cgaggtccac
      961 ccgcaggccg cgctggccgg gggccgagat gcggaacgcg gtgagctgcg ccgtgaggtg
     1021 ccggaagcgc cgcacgcgac ggcggccgtc cgacccgaac gcgtcgatct cgagcagcga
     1081 atcgccgcgt gtgggccgtg actggagccg cagcaccaga tccccgtcga cgattgtgac
     1141 gttgcgcagc accacgagcg gcttgggccc gcggcgtggc cccttcgacg gctcgcccag
     1201 gtgcagcagc tcctcgatgt tcaggcggcc gttcttgtgc tgcacgatgt tgaagtaggg
     1261 gtgctcgagc cgcacccgct ggagcacgat ctggcccgcc acaaaatcga gcagattgta
     1321 gtcgagctcg gcgcgcggca gccacgcgac cagcgtcgtg tcgtggtcgt agagccggac
     1381 gtcggcgtac gcgagtcccg tgagcagcga gcccgacgtg ctccccagct ccacgcgccc
     1441 cccgaccacc gattccagcg ccgccgtgga gacccgcgtc agcaggtcat ggccggcctt
     1501 cgtacccacc agcgccgaca gggcgcccag gaacgcggcc agcaggccga ccagggacca
     1561 cagcaccacg ctcaggctgc gccgcaccac tagaacacct gcccgatcgc gaactgcagc
     1621 gtgagccgat tgagcagcga cccggagcgt ctgggctgat agttgagccg gtagtccgtc
     1681 agcgttcccg ctgatctatt gaggagcttc agcctgcccg attcccggtc gtagccgttg
     1741 taggcgacgt ccacgcggat cgggccgagg ggggtcgtca cccggacgcc cacgccgggg
     1801 gtcacgcgca atgcgcgcaa cgagaagaga tcgccctgct gctggtacac ctgtcccacg
     1861 tccacgaaga acgcgacccg caggcgatcg gggaagaccg gagcgggcac tcgcagctcc
     1921 gcgttggcca gcaggatggc attgccgcca atcggcgaca ccgtcgcgtt gttgtagacc
     1981 gtgtcgccgc ccacgaccga gtacgcgcag tgggcggcgt cggcgcacgt agtgtcggtg
     2041 atcacgtaga ccaccggccc cagctcgttg gccgggtacc cgcgcaccga gttggggccg
     2101 ccggcgtaga accgctgctc gggcgggacg aactgcaccc gctgcccggc ggcggtgacg
     2161 ccggccggga cgagcgcccc ggcccggacg cgccaggcga acacgcctcg ccggccgaac
     2221 ggcatgtagc gcgcgacctc cagctccccg cggttgaatt cgtacagggt atccgagccg
     2281 agcaggcgcg aggaatgcag cagcgtgatg ccgacccgcc ctccccggct gggatcgagc
     2341 aggaagtcct cggtgcgcag gcccgcggtc agggtcagcg acgcgaaccg gtgcgggttc
     2401 cgcaggtaac gcagcgtgga gtcgtcgcac acgttgaaca cgctgcagaa ggcggcgtcg
     2461 ttggcgtcgg tgcggcctcg tgacaatcca taaatgaagg tgagcggcac ccggcgccgg
     2521 gcgttgagcg tcacccccac gttggccccg acctggatgc gggtgtacgt ccgcacctcc
     2581 gagcgccgct cggcgaacac gccgatactc gccgaatggc ggggcgagag aaacgtgggc
     2641 tggagcaggg tcagcgcgac gttgtagttc agggtatcgg aggtcgagtc gccgtacagc
     2701 ggaaagcaca gggactgctg ggtatgcttg aaacccaggt tgagcggcgc gccgacgccg
     2761 atcttcgata gccgtgcgct caggtccagc acccgcgcat cgcccagaaa gccgtaggac
     2821 gaccacccgc cctgcgcccg gaagcagtcg agggtgccgt agcccgcgcc caggccgacg
     2881 cgttgccgcg acccctcggt gacgcgaacg agcacgtcca ccgtcgaatc gccgtcggcc
     2941 ggtggcacgc tgtcacgcag gctcaccgac gcggcgcgga acatcccggc gccgtagagg
     3001 tcgcgctggc tctgatacag gagaccctcg ccatactgcg ccccggtgtg caccgacagc
     3061 gcgtgcagca cggtcgccgt atccacgcgc tgcaggcctt cgaccacgac ccggccgatg
     3121 cgcatgtacg gacccggcac cgcctcgaaa cggacgtcgg ccgccagttc cgccgcctcg
     3181 gcgtcgaagt tgcgcagcac ctgggcgtag ggaaacccgc ggttgcgcag ccacgccgcc
     3241 atggtgtcgg ccgaggcctg catgagaaag cgattgaagg ggtcgccgac cttgagcggc
     3301 agcgcgcgcc gcaatggccg ctcgtccacg atgccgctca caccttccag cgcgagctgc
     3361 cggacgcgga cgggcgcgcc ctcgaagatc cggaaggtga tgtacacgtc gcggggcgta
     3421 cgccgcacgc tcgtgtcgac caccgcctgc atgtacccgc tctggcgata ataaaggatc
     3481 aggcgcacca cgtcgcggcg gaactccagc tcgtcgaagt agcgcttcgc gcccagtccg
     3541 agcgaccgca ccagcgccgc ccgggcaaac cagctggatt tcgaggtggc gatgacggtg
     3601 cccagggtat aatcgtcgat cgcatggttt ccgacgaagt cgagctgacg caccacccgc
     3661 tccggctcct gctgcgccgc gatcggagcg gtgggcacgg ccggcacgca caacagcacg
     3721 gcgagagcga aacgacgcag gaaacgcaat tgacggtata tgttgggccc cgtatgttgg
     3781 ccggccacta ccccgactgt caaccttttc gacaccgaac ttgatgcccc ggttccgggc
     3841 ctatccgacc gctctgctca cactcctcgc cgcctgcagc ggcgggcggg gacgcggtgc
     3901 cgcgggggcg ccgctcgtct actacgagag ctacgatccg cgctcgctcg atcccgccct
     3961 ctcgaccgac gtgccgaccg gcgagatggt cacgctgctc tacgacggcc tcacccgctt
     4021 cggcgccgac ggctcgctcc tgccggcgct cgccgaccgc tggagcgtcg atgccgcggg
     4081 gctccggtac gtattccacc tgcggcccgg cgtgaagttc cacgacggtg ctccgctcga
     4141 ccccgaggcc gtgcggcgca gcttcctccg ggtgctggat ccgaaatcga ccggcgggcg
     4201 cgcctggccg ctgctcccga tcgacggcgc atcggcctac gccgcgggca cgacggaccg
     4261 ggtccgcggc atcgaggtcc tgggcgacac gggcgtcgcg ctcacgctga cccagccgct
     4321 cgccgtgttc ccgaaattcc tggccatgcc ggtcgcgtcg atcgccggcc cggactcggg
     4381 gaaggatctg tcgacccggc ccaacggcac cggggcatgg cgcttcgcca gctgggcgca
     4441 cgacgacgcg ctgcacttcg cgcgcaaccc cgcctactgg ggcggtgcgc cgcaggccga
     4501 ctcgctcgtg gtgcgcatca tccccgacca gctggtgcgc gctgccgagt tcgagtccgg
     4561 ccggctggac gtggtggagg tgcccttcgg cgagacggca cgctggcgcg agcgccaccc
     4621 ggcctggctg cagcagaagc cggccctgcg cgtcacctac atcgcgctca acacgcgccg
     4681 tggtccgctg cgcgacgccc gcgtgcgcca ggcgatcaac catgccgtga acgtggacca
     4741 gatcctggcg accgtctgga gcggcagggg gatccgcgcc cgcggggcca tcccccccac
     4801 cctcgcgggg ggcgacacga cgcgcccggc gttcgcctac gacccggcgc tggcgaagca
     4861 gctgctggcc caggcagggc atgcgggcgg gttcgcgctc cagctctggc gcaccgcgga
     4921 caacgagacc ctgggacgcg tggcgcaggc ggtgcagtcc gacctcgccg cggtcggcat
     4981 ccgggtagag atcgtcacgc gcgacgcccc gagccagcgc gaagcggcgc gtgctgggaa
     5041 cgccgacatg gccctgctcg actggtgggc cgactacccc gacggcgaca acttcctcta
     5101 tcctctgttt gcgagcgcca gcttcggccc gggcggcaac tacgctttct atagtgaccc
     5161 ggtcaccgac tccctgatcc gcaccgcccg ccgcacccag gacgacgcgg cgcgcaccgc
     5221 cctgtaccac gccatcgacc agcgcgtcta tcaggcggcg ccctggctct acctctggtt
     5281 tccggtggat ctctgggccc aatcgccgtc gatcacgggg tgggagctgc cggtgatctt
     5341 caacgggcag cgctggaccg cggtgcgacg cgcgtccccg caaccgtgac cggcgtcctc
     5401 ggccggcgcc tgctgctcgt cgtcccgacg ctgatcggcg tgctcgtcgc cgccttcctg
     5461 ctgctcgaag tcgccccggg tgacccggtc caggcgatgg tgggcgagcg agccgactcg
     5521 gccacgatcg cccgattgcg cgcagaactg cgtctcgacg atccgctgcc gctgcggttc
     5581 ggccattacg tgtggggcgt agtgcgcggc gacctcggcc gctcctacat cacccggcgg
     5641 ccgatcgccc aggacctgct ggagcggttc cccaagaccg cccagctcgc gctcaccgcc
     5701 atgaccttcg ccgcgctcgc cgggatctcc ctcggcgtgg tcgcggcgat ccgccccggc
     5761 ggcgccgccg accgggccgc cagcctgctc gcctacctgg gggtctcctt cccggtgtac
     5821 tgggtcgcgc tcctcatggt gctgctcttc gccgtgacct tgcgctggtt cccaccatcc
     5881 gggtcggggg ggctcgcgtt cctcgtgctc ccggcggcga cgctcggcat gcgctcggtc
     5941 gcggt
//
LOCUS       DBNGZW010000454         3803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_99535, whole genome shotgun sequence.
ACCESSION   DBNGZW010000454 DBNGZW010000000
VERSION     DBNGZW010000454.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_99535"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1081
                     /locus_tag="AC16JC_10710"
     CDS             <1..1081
                     /locus_tag="AC16JC_10710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165207.1"
                     /translation="MVSGLYDPATTHRLALTDGGVYDNQGLSALVEEECTHIVASDAG
                     GSLPLERRPSAGRIPMLARIGAVLMADVRDRQLHELREWLRASEAAGAARPGSRLEAV
                     AFFEMASDALAAVPAGPKPPEPHPDADAIAALRTDLDAFSPVEIDALIYQGYQLADRY
                     VRTHLGSAFLGDPKDAPPPLVVVAPDSATSPRLAAILRAGARRLFRAVALSRPLRWTL
                     YAAGLASLVLLGLRDLSVGQLGERLVNGLRWLGTHPFLNPRWELEWSSPKGVLLLLVG
                     AILLTIVWFCWPKFEAALANMLAGRRGDSLQGHVRASRIARAVGQWRRNIWWPLGLAP
                     LWAALGLAAAATVVFVFDLIPARRK"
     gene            1157..2071
                     /locus_tag="AC16JC_10715"
     CDS             1157..2071
                     /locus_tag="AC16JC_10715"
                     /inference="COORDINATES: protein motif:HMM:NF016291.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HIR5165208.1"
                     /translation="MNERDHETIVTVAYLAALADGRTAPAEHDELRAALGRLGVADVD
                     AVAQRGSAVRAPLADLARQLSGDDARRLAFETALVVCHADGPLNRQEADFLGALRSAM
                     GLAPATLAELEQSAAGLAAARTAGPVEVRSGKPPSDDALDQTILRQAMIASAVELLPD
                     KLANVVILPLQLRLVYQIGQQYGQKLDANQIKDLAGTLGLGAAAQVMEGVVRKLVGGV
                     TGGLFGGLVGGAGGMAAGVAVTFAATYALGHVAKQYYAQGRRLSTQDLRELFQRFQGD
                     AKEIFPKVQDEIKAQAKSLNLKSLLEGI"
     gene            2068..2604
                     /locus_tag="AC16JC_10720"
     CDS             2068..2604
                     /locus_tag="AC16JC_10720"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006975965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR5165209.1"
                     /translation="MTDASIARMPSPVGELLLLANSNGLAGVWFETSRHGRPEGAEWP
                     ADDGQGRAGAILARAREQLEEYFARHRTAFDLPLAPVGTPFQLRVWNALRDIPFGTTE
                     SYGGLARRIGAPTSAARAVGAANGQNPIPIIVPCHRVIGATGDLTGFGGGLERKRWLL
                     THEGAISAPLDISTFVDV"
     gene            2613..2939
                     /locus_tag="AC16JC_10725"
     CDS             2613..2939
                     /locus_tag="AC16JC_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5165210.1"
                     /translation="MTTATLPDSTRAARWVHALSDPTRLEIVRMLSHGERCVCELQDA
                     LSAQQSRLSFHLRVLKESRFVTDRKDGRWVHYSLNREALDEIAAFASAVQPGKHAGSC
                     RSGCCS"
     gene            2993..>3803
                     /locus_tag="AC16JC_10730"
     CDS             2993..>3803
                     /locus_tag="AC16JC_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HIR5165211.1"
                     /translation="MSDTIKQTVREKYGQAALRVLAGSGSSCCGASASGESCDPVTSN
                     IYAPGETAGLPETAVLASLGCGNPTALAELKPGDVVLDLGSGGGIDVLLSARRVAPGG
                     KAYGLDMTDEMLALARDNQRKAGVENVEFLKGDIEHIPLPDASVDVIISNCVINLAAD
                     KRRVLAEAFRVLKPGGRFAVSDVVVRGTLPLEVRRNMELWVGCVAGALEEDEFKGLLA
                     ASGFSEVSIEPTRVYAFEDARAFLAGTGLDADVLAREIGGRVMGAFVRATKP"
BASE COUNT          551 a         1308 c         1350 g          594 t
ORIGIN      
        1 catggtgtcc gggctgtacg acccggccac gacacaccgc ctggcgctga ccgacggcgg
       61 cgtgtacgac aaccaggggc tgtcggcgct ggtcgaggag gagtgcaccc acatcgtcgc
      121 gagcgacgcg ggtggctcgc tgcccctgga gcgccggccg agcgccggcc ggattccgat
      181 gctggcgcgc atcggcgccg tcctcatggc cgacgtgcgc gatcggcagc tccacgagct
      241 gcgcgagtgg ctgcgggcaa gcgaggcggc cggcgcggcc cgcccgggct cgcgtctcga
      301 ggccgtggcg ttcttcgaaa tggcctcgga cgcgctcgcg gccgtcccgg cagggcccaa
      361 gccgccggag ccccatccgg acgccgacgc catcgcggcg ctgcgcaccg atctcgacgc
      421 cttctccccg gtcgaaatcg acgccctcat ctaccagggg taccagctcg cggaccgcta
      481 cgtgcggacc catctcggat ctgccttcct gggtgatccg aaggacgcgc cgcctccgct
      541 ggtcgtggtg gcgcccgaca gtgcgacgtc gccccgcctc gcggccatcc tccgggcggg
      601 ggcgcggcgg ctgttccgcg cggtcgccct gtcacggccg ctgcgctgga cgctgtacgc
      661 ggcgggcctc gcgtcgctcg tgctgctcgg tctccgggac ctgtcggtcg ggcagctggg
      721 cgaacgtctc gtgaacggat tgcgctggct cggaacccat ccgtttctca atccgcgatg
      781 ggaactcgaa tggtcgtcgc cgaagggtgt gctgctgctc ctggtcggcg ccatcctcct
      841 gaccatcgtc tggttttgct ggccgaagtt cgaagcggcg ctcgcgaaca tgctggccgg
      901 ccgccggggt gacagcctcc agggccacgt gcgggcctcc cgcatcgcgc gcgcggtggg
      961 gcagtggcgc cgcaatatct ggtggccgct cgggctcgct ccactgtggg cggctctggg
     1021 cctcgcggcg gcggcgacgg tggtcttcgt gttcgatctg atcccggccc gccgcaagta
     1081 gcggccccct ttcacggggc cgcgggtatg taagattctc gctgtatact ccaaccttcc
     1141 gcacggacct cgacctatga acgagcgcga tcacgaaacc atcgtcaccg tcgcctacct
     1201 cgcggccctg gccgacggcc gtacggcgcc ggcggagcac gacgagctgc gcgccgcgct
     1261 gggacgtctg ggcgtcgcgg atgtcgacgc cgtcgcccag cgcggcagcg ccgtccgggc
     1321 gcccctggcg gacctcgccc ggcagttgtc cggcgacgac gcccgccggc tcgccttcga
     1381 gaccgccctc gtggtgtgtc acgccgatgg accgctcaac cgccaggagg cggattttct
     1441 gggggcgctg cgtagcgcaa tggggctcgc ccccgccacg ctcgcggagc tggagcagag
     1501 cgccgcgggc ctcgccgcgg cccgcaccgc cggacccgtc gaggtccgct cggggaagcc
     1561 cccctccgac gacgcactcg accagacgat tctgcgacag gcgatgatcg ccagcgccgt
     1621 cgagctcctg cccgacaagc tcgccaacgt cgtgatccta ccgctgcagc tgcgcctggt
     1681 ctatcagatc ggccagcagt acggtcagaa actcgacgcg aaccagatca aggacctggc
     1741 cggcacgctg ggtctcggtg cggcggcgca ggtcatggaa ggcgtcgtgc ggaaactcgt
     1801 gggaggggtg acgggtgggc tgttcggcgg tctggtaggt ggggcgggtg gcatggccgc
     1861 gggcgtcgcc gtgacgttcg ccgcgaccta cgcgctcggc cacgtcgcga agcagtacta
     1921 cgcccagggg cggcggctca gcacccagga cctgcgggag ctcttccaac gctttcaggg
     1981 agacgccaag gagatcttcc ccaaggtcca ggatgagatc aaagcccagg cgaagtcgct
     2041 caacctgaag agcctgctcg aaggaatatg acggacgcca gcatcgcccg catgccgagc
     2101 cccgtcggcg agctgctgct gctcgcgaac agcaacgggc tggcgggggt gtggttcgag
     2161 acctcccgcc atggccggcc ggagggcgcg gagtggcctg ccgacgacgg gcaggggcgg
     2221 gcaggggcga tcctggcgcg cgccagggag cagctcgagg agtacttcgc ccggcatcgc
     2281 accgcgttcg acctgcctct cgccccggtg gggaccccgt ttcagctccg tgtctggaac
     2341 gcgctgcgcg acatcccgtt cggcacgacc gagagttacg gagggctggc ccggcggatc
     2401 ggcgcgccca cgtcagcggc gcgggccgtg ggggcggcga acgggcagaa tccgatcccc
     2461 atcatcgtac cgtgccaccg cgtgatcggg gcgaccgggg atctgaccgg tttcgggggc
     2521 gggctggagc gcaaacgctg gttgctgacc catgaagggg cgatttccgc ccctcttgac
     2581 atatcaacct ttgttgatgt ataattgacc ccatgacgac cgccacactc ccggacagca
     2641 cgcgcgccgc gcgctgggtc catgccctct cggatccgac gcggctcgaa atcgtgcgca
     2701 tgctgtcgca cggcgagcga tgcgtctgcg agctccagga cgcgctgagc gcccagcagt
     2761 cacggctctc gttccatctc cgagtgctca aggagtcgcg gttcgtcacc gaccgcaagg
     2821 acggccgctg ggtgcactac tcgctgaatc gcgaagcgct ggatgagatc gccgcattcg
     2881 cctcggcggt gcagcccggg aagcacgccg ggagctgccg ttccgggtgc tgttcgtgag
     2941 gcgttttttt cgccgtacgc atcaacattt cttgatagac gaggcccaga acatgagcga
     3001 cacgatcaag cagacggtgc gagagaagta cggccaagcc gcgctccgcg tactggccgg
     3061 aagcggctcg agctgctgcg gcgcgagcgc gtccggggag agctgcgacc ccgtgacgtc
     3121 caacatctat gcgcccgggg aaaccgccgg actgccggag accgccgtcc tcgcttccct
     3181 tggctgcggc aatcccacgg cgctggcgga gctgaagccg ggcgacgtgg tactcgacct
     3241 gggctcgggc ggcgggatcg acgtgctcct ctcggcgcgc cgcgtggcgc ccggagggaa
     3301 agcctacggc ctggacatga ccgacgagat gctcgccctg gcgcgcgaca accagcggaa
     3361 agccggcgtg gagaacgtgg agttcctcaa gggggacatc gagcatattc cgcttcccga
     3421 cgcgtcggtg gacgtcatca tctccaactg cgtcatcaac ctcgccgccg acaagcgtcg
     3481 cgtgctggcc gaggcgttcc gcgtcctgaa gcccggcggc cgctttgccg tgtccgacgt
     3541 cgtggtgcgc ggtaccctgc ctctggaagt ccggcgcaat atggagttat gggtcggatg
     3601 cgtcgccgga gcgctcgagg aggacgagtt caagggcctg ctcgcggcga gcgggttcag
     3661 cgaggtctcg atcgagccga cccgcgtgta cgccttcgag gacgcccggg cgtttctcgc
     3721 cggaacgggt ctggacgccg atgtgctcgc ccgggagatc ggcggtcggg tgatgggggc
     3781 gttcgtgcgc gccaccaagc cat
//
LOCUS       DBNGZW010000455         2389 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_712762, whole genome shotgun sequence.
ACCESSION   DBNGZW010000455 DBNGZW010000000
VERSION     DBNGZW010000455.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_712762"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="AC16JC_10735"
     CDS             complement(<1..1616)
                     /locus_tag="AC16JC_10735"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR5165212.1"
                     /translation="MNTSTLRLALAAAFLAPHRAPAQQPPASPAQASATIRAGVPDTS
                     PFRILPLAPPDVYRTASGMPGPSYWQQRADYTIRASLDTASHTIRGEETIRYTNNSPD
                     TLRFVWLQLDMNAGAPDTRFAPLANPLARAEPGFRAGATIERIAALRSATPQARAATT
                     ALTWRVNSTMMRVDLDRPLPPHGTVSLAVAWHHQIPRIGRTGRQQFPQGWLYQVAQWY
                     PRLAVYDDVRGWNTDQYIGSSEFYLEYGDFDVTLTLPAGFTVAATGVLRNPLEVLPAK
                     IRARLAAAARADTIVHIIPADEVGTPGLLPAGSGPTRAWHFTATNVRDFAWGTAPNFL
                     WDATSWDGILMQALYPPSVADVWKTAADMNRHAVMIHSRWLHYPWPTAISVEGPVGGM
                     EYPMMTFDNARDERELYYTIAHEHGHEWFPMMVGSQERLYPWMDEGFNTFIDWFSFRD
                     RYPGDTTRIQALEFGSMRAYQDFLRTYRGVETPIMESQDRTPNGLMSGWNAYGKPAVA
                     LHFLREQVVDSSAFDDAFREYVRRWAFKHP"
     gene            1700..>2389
                     /locus_tag="AC16JC_10740"
     CDS             1700..>2389
                     /locus_tag="AC16JC_10740"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR5165213.1"
                     /translation="MRKVLVWVAVILAGRVATLVSQQPIGPMVDSIARQVLEATGVPS
                     ASVAVVQDGKLVYTQAYGSAQVEPAVPARVDMRYSIGSISKQFAAAAILLLQQDGKLS
                     LDDPVARFLPDLTRAREVTIRQLLSHTSGYQDYWPQDYVPLFMLESVTAEGILDRWAK
                     KPLDFEPGTRWQYSNTNYVAAGLIVERSSGMPFFEFLRTRILAPLGMQSVVNIDLGRL
                     PQSDPTGYLRYA"
BASE COUNT          370 a          762 c          868 g          389 t
ORIGIN      
        1 tgggggtgct tgaacgccca gcgacgcacg tactcgcgaa acgcgtcgtc gaacgcgctc
       61 gagtccacga cctgctcgcg caggaaatgg agcgccaccg ccggcttgcc gtaggcgttc
      121 cagccgctca tgaggccgtt gggagtgcgg tcctgggact ccatgatcgg cgtctcgacg
      181 ccgcgatacg tgcgcaggaa gtcctggtag gcgcgcatgg agccgaattc cagtgcctga
      241 atgcgcgtcg tatcgccggg atagcggtcg cggaagctga accagtcgat gaaggtgttg
      301 aacccctcgt ccatccacgg gtagagccgc tcctgggagc ccaccatcat ggggaaccac
      361 tcgtggccgt gctcgtgggc gatggtgtag tagagctccc tctcgtcccg cgcgttgtcg
      421 aaggtcatca tggggtattc catgccgccg accggccctt ccacgctgat ggcggtgggc
      481 cacgggtaat gcaaccagcg ggagtgaatc atcacggcgt ggcggttcat atccgccgcc
      541 gtcttccata cgtccgccac gctgggcggg tacagcgcct gcatcaggat gccgtcccag
      601 ctggtcgcgt cccagaggaa gttgggcgcc gtcccccagg cgaagtcgcg cacgttggtc
      661 gcggtgaagt gccaggcgcg cgtcgggccc gagccggccg ggagcagtcc cggggtcccg
      721 acctcatcgg ccgggatgat gtggacgatg gtgtcggcgc gagccgcggc cgccagtcgg
      781 gcccggatct tcgccggcaa cacctcgagc ggattgcgca gcacgccggt ggcggccacc
      841 gtgaagcccg cgggcagggt gagcgtgacg tcgaagtcgc cgtactcgag gtagaactcg
      901 ctcgacccga tatactgatc ggtgttccag ccgcgcacgt cgtcgtagac cgccaggcgt
      961 ggataccact gggccacctg atacagccag ccctggggga actgttgcct cccggtgcgg
     1021 ccgatgcggg ggatctgatg gtgccaggcg acggccagcg acaccgtgcc gtggggcggc
     1081 agcggccggt cgagatcgac gcgcatcatg gtactgttca cgcgccaggt gagcgcggtg
     1141 gtcgccgccc tggcctgtgg ggtggccgag cgcagcgcag cgatgcgctc gatcgtcgcc
     1201 ccggctcgga acccgggctc cgcgcgcgcc agcggattgg ccagcggcgc gaagcgcgtg
     1261 tccggcgcgc cggcgttcat gtcgagctgc agccagacga agcgcagcgt atcgggcgaa
     1321 ttgttcgtgt agcggatcgt ctcttcgccg cggatcgtgt ggctcgcggt gtcgagtgag
     1381 gcgcggatgg tgtaatcggc ccgctgctgc cagtatgagg gtcccggcat gccggaggcc
     1441 gtccggtaga cgtcgggcgg cgccagcggc agtatgcgga acggggaggt atccggaacg
     1501 ccggcgcgaa tcgtcgcgga cgcctgcgcc ggactggcag ggggttgctg ggcgggcgcc
     1561 cggtgcgggg cgaggaatgc ggcggccagc gcgagccgaa gggtcgatgt gttcatggag
     1621 tgcaagatag gagactttcg ctcgaacggc acggaccacc gctcggcaac gccatgactc
     1681 tctacatcag gagccgatca tgaggaaggt gctcgtgtgg gtggcggtca tcctcgccgg
     1741 ccgagtggcg acgctcgtgt cgcagcagcc gatcgggccc atggtggatt cgatcgcccg
     1801 gcaggtgctg gaggccaccg gtgtgccgag tgcgtccgtg gccgtcgtgc aggacgggaa
     1861 gctggtctac acgcaggcat acgggtcggc gcaggtcgag ccggcggtgc ccgcgcgcgt
     1921 ggacatgcgc tacagcatcg gctcgatcag caagcagttc gccgcggccg cgatcctgct
     1981 gctgcagcag gacgggaaac tgtccctcga cgacccggtc gcgcggtttc ttcccgatct
     2041 gacgcgcgcg cgcgaggtca ccatccggca gctgctgtcg cacacctcgg ggtaccagga
     2101 ctactggccc caggattacg tgccgctctt catgctcgaa tcggtcacgg ccgagggcat
     2161 cctcgatcgc tgggcgaaga aaccgctcga tttcgagccc ggcacccggt ggcagtacag
     2221 caatacgaac tacgtggccg cggggctgat cgtggagcgg tcgagcggga tgcccttctt
     2281 cgagtttctc cgcacacgca tcctggcgcc gctcgggatg cagagcgtgg tcaacatcga
     2341 cctcggccgc ctgccgcagt ccgacccgac gggctacctg cgctacgcg
//
LOCUS       DBNGZW010000456         2152 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1268493, whole genome shotgun sequence.
ACCESSION   DBNGZW010000456 DBNGZW010000000
VERSION     DBNGZW010000456.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1268493"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..918)
                     /locus_tag="AC16JC_10745"
     CDS             complement(<1..918)
                     /locus_tag="AC16JC_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR5165214.1"
                     /translation="MRHRRPRPFAARLPVLLFLFVTSPAATLVAQRRAAPSSGGSAAA
                     IDTARYAQLHFRHIGPEGNRVTSVAGVPGDPGTYYAGAASGGLWKTVDGGVHWDAVFD
                     DQPVSSIGALAIAPSDPNVVWAGTGEPFIRSNVSVGWGVYKSTDAGKHWTKVGLDSTG
                     RISRIVVHPTNPDIVYVAALGHLYGPQPERGIYRTADGGKTWERVLFVNDSTGASDLV
                     MDPNNPRILFAGFWQVELHTWIRSSGGKGSGIWTSRDGGTTWTRVTGSGLPTRPVGKI
                     GLAMTRANSNRIYALVETGDGVPAVNIARP"
     gene            complement(979..1575)
                     /locus_tag="AC16JC_10750"
     CDS             complement(979..1575)
                     /locus_tag="AC16JC_10750"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR5165215.1"
                     /translation="MTRSGALALVLALAGAGSLCGQRRGDAAADRYDRIFRKYTKRYF
                     GPAFDWRRFKAQGLAESGLDKTARSRDGARGIMQLMPSTMEAIQSSAPDLVSIDVPEL
                     NIAAGIRHDRALWNIWERAATPHDRRRFMFASYNAGSRTIARAQRVARRHRLDPTRWD
                     SIRRIGWRVWRWRQVQTMHYLRKIDMYLGRLDEDGKLR"
     gene            complement(1572..1775)
                     /locus_tag="AC16JC_10755"
     CDS             complement(1572..1775)
                     /locus_tag="AC16JC_10755"
                     /inference="COORDINATES: protein motif:HMM:NF015927.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HIR5165216.1"
                     /translation="MEWHIVGLNFLYAGLGVALMYVAYRLFGVLTPRVDFAAELKRGN
                     VAVAIFIGALFVALAIIIGGALN"
     gene            1823..>2152
                     /locus_tag="AC16JC_10760"
     CDS             1823..>2152
                     /locus_tag="AC16JC_10760"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR5165217.1"
                     /translation="MTKHAILLAAAFPAALAAQVRDTVSDTVTLPPVTVTATRRTESL
                     FSVPLAVTRITRGAWQGSRALGLDDALALVPGVLAQSRYGSGDVRLVIRGFGARGAGD
                     RSNAGTSR"
BASE COUNT          306 a          785 c          740 g          321 t
ORIGIN      
        1 gggacgggcg atgttcacgg cgggcacgcc gtctcccgtc tcgaccagcg cgtagatccg
       61 gttcgagttc gcccgcgtca tggcgagccc gatcttcccc accggcctgg tcggcagccc
      121 gctccccgtg acgcgggtcc aggtcgtgcc gccgtcgcgc gaggtccaga tgccgctgcc
      181 cttgccgccg ctcgagcgga tccaggtgtg cagctccacc tgccagaatc cggcgaacag
      241 gatccgcggg ttgttgggat ccatcaccag gtccgacgcg ccggtgctgt cgttcacgaa
      301 cagcacccgc tcccaggtct tgccgccgtc ggcggtgcga tagatgcccc gctcgggctg
      361 cggcccgtac agatggccca gcgccgcgac gtagacgatg tccgggttgg tggggtgtac
      421 gacgatgcgc gatatgcgtc cggtgctgtc gagccccacc ttggtccagt gcttgccggc
      481 gtcggtggac ttgtagacgc cccacccgac cgacacgttg gagcggatga acggctcgcc
      541 cgtcccggcc cacaccacgt tggggtccga cggcgctatg gccagcgcgc cgatggacga
      601 cacgggctgg tcgtcgaaca ccgcgtccca gtgcacgccg ccgtccacgg tcttccacag
      661 gccgcccgat gccgcgccgg cgtagtaggt gcccgggtcc ccgggcacgc ccgccaccga
      721 ggtgacccgg ttcccctccg gtccgatgtg gcggaagtgc agctgggcgt agcgcgccgt
      781 gtcgatcgcg gcggcgctcc cgcccgatga cggcgccgcg cgccgctgag ccacgagcgt
      841 ggcggccggg ctggtgacga acaggaatag cagaacggga aggcgggcgg cgaagggccg
      901 cggacggcgg tggcgcacgg ctgctcctca cggaggggga aatcgcggga gcaaagatgc
      961 acgtcgggag gcccctcgct accgcagctt cccgtcctcg tccagccggc cgaggtacat
     1021 gtcgatcttg cgcagatagt gcatcgtctg cacctggcgc cagcgccaca cgcgccaccc
     1081 gatccgccgg atgctgtccc accgcgtcgg gtcgagccgg tggcggcgcg ccacgcgctg
     1141 ggcccgcgcg atggtgcggc ttcccgcgtt gtagctcgcg aacatgaagc ggcgccggtc
     1201 gtgcggcgtc gccgcccgtt cccagatatt ccataacgcc cggtcgtgcc ggatgccggc
     1261 cgcgatattc agctcgggga cgtcgatcga cacgagatcg ggggcactcg actgaatcgc
     1321 ctccatggta gacggcatga gctgcatgat gccccgcgcc ccgtcgcggc tgcgcgcggt
     1381 cttgtcgagc ccgctctcgg ccagcccctg ggccttgaaa cgccgccagt cgaacgccgg
     1441 cccgaaatac cgcttggtgt acttgcggaa gatccggtcg tagcggtcgg cggccgcgtc
     1501 gccgcgacgc tggccgcaca ggctcccggc accggccagc gccaggacga gcgcgagggc
     1561 gccgctccgc gtcagttgag cgccccgccg atgatgatcg cgagcgccac gaacagcgct
     1621 ccgatgaaga tcgcgaccgc gacgtttccc ctcttcaact ccgccgcaaa gtccacgcga
     1681 ggcgtgagga cgccgaacag gcggtacgcc acgtacatga gcgcgacgcc cagtccggcg
     1741 tacaggaagt tgagcccgac gatgtgccac tccatgcccg cctcccttga cgccccacgc
     1801 tccggggcgt aagtcctcgc acgtgacgaa acacgcgatc ctcttggccg cggcgttccc
     1861 cgcggcgctc gccgcccaag tgcgcgacac cgtgagcgac accgtgacgc taccgccggt
     1921 cacggtcacc gcaacgcgtc gcacggaatc gctcttttcc gtgccgcttg cggtgacccg
     1981 tatcacgcgg ggtgcatggc aggggtcgcg ggccctggga ttggacgacg ccctggcgct
     2041 cgtgccgggc gtcctcgcgc aatcgcgcta cggttcgggc gacgttcgcc tcgtgatccg
     2101 cggcttcggc gcccgtggcg ccggcgaccg gtccaacgcc ggaacctcgc gc
//
LOCUS       DBNGZW010000457         7848 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1394591, whole genome shotgun sequence.
ACCESSION   DBNGZW010000457 DBNGZW010000000
VERSION     DBNGZW010000457.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7848)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7848)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7848
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1394591"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..327
                     /locus_tag="AC16JC_10765"
     CDS             <1..327
                     /locus_tag="AC16JC_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013394102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HIR5165218.1"
                     /translation="LTHFAFVLFVIAGALLLRHSRWWAVLHVPAFAWGALIEFRGWIC
                     PLTPLENTLRARAGQAGYAGGFVEHYITRLLYPEGLTRGIQVALGALVLAGNLVLYWW
                     LWRRRG"
     gene            330..809
                     /locus_tag="AC16JC_10770"
     CDS             330..809
                     /locus_tag="AC16JC_10770"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5165219.1"
                     /translation="MSGTGTIRRLAGEAELGACARMMAQSEPWITLGRTLETSTANLR
                     DPGKEVYVVEDEGRISGFVVIDLRGPFPGYIQTVCVSPDARGRGHGTRLIAWAEERIF
                     RDSPNVFMCVSTFNPAARRLYERLGYTAVGELSDYIVPDHGEILLRKSRGPWSEFRR"
     gene            806..3112
                     /locus_tag="AC16JC_10775"
     CDS             806..3112
                     /locus_tag="AC16JC_10775"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR5165220.1"
                     /translation="MRPDLRLGITIAMAATAPPLAAQDSVPARADYGAAAAVLTRFID
                     HEMADKDLPAVSIVLVDDTAIVWARGFGFARPRDSVPATALTVYRVGSVSKLFTDIAL
                     MRLVDRGTVDLDAPVTRWLPDFHPVNRFERPVTLRQLMTHHAGLVREPPVGSYFDSTA
                     PPLSAIAASLNRTALVYRPGTRYKYSNAALQVVGYVLERATHEPFPRYLKDSVLQPLG
                     MTRSAFFEPAPETARDLAAAFMWAPDGRRFAAPTFRRNAPSGALYTTVADLGRFLAGL
                     FADSNPVVPHTTLERMWADSGLGFFVSRFDGHRRVGHDGAIYGFATTLAALPYEKLGV
                     AVVTTLDGANTVTGRVADAALRVLLARRAGAPLPEPPMTAPLPPGTARAAAGHYVRGA
                     RSVDLLSRSGELLVARDGSAVPARLRTLGDSLVFDDVLTFGGAVRLPAGAVVVDGDTF
                     ARAPAPRPADVSPRWKGLLGEYGWAYETLFAYERAGRLHVLIEWTEDDAMTPVSDTVF
                     AFPATSMYGGERLVFRGTRSAGGRATTVIAGAVAFPRRVVGPQGGGQLKVTPLHPVAE
                     LLAAARTATPPAESGSFRAPELVDLATLDSTIHFDIRYATTNNFLGSVFYSTAHAFLQ
                     RPAAEALLRAARSLRARGFGLLIHDGYRPWYVTKTFWDATPAGLRWLVADPARGSRHN
                     RGCAVDLTLYDLRTGRPIDMGGTYDETTERSYPDYPVTADLERWHRDELRRAMEDEGF
                     TRVPNEWWHFDYRDWRSYPILNVPFEDL"
     gene            3109..3771
                     /locus_tag="AC16JC_10780"
     CDS             3109..3771
                     /locus_tag="AC16JC_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HIR5165221.1"
                     /translation="MKTALIAGATGLVGSRCLARLLAEPAYERVIALVRRPLPHDDPR
                     LEARVIDFGTLGAAGTAFPAASDVFCCLGTTMKQAGTEEAFRLVDFTYVVSLAAQSLE
                     HGARQFLLVSSIGANRASAFFYPRVKGETEDAIAALPFEGRQIFRPSILVGERAEHRP
                     GERAWIAAMKGGAFLMLGPMRKYRPIGAAAVAEAMVRVALRAPRGVNVYESNEIARIA
                     RG"
     gene            3815..4564
                     /locus_tag="AC16JC_10785"
     CDS             3815..4564
                     /locus_tag="AC16JC_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008103040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIR5165222.1"
                     /translation="MSMANPLLSDKAFQRAGQYAQATTGELMTVEGAINKSAFLLLLV
                     VGAGTWVWTRYYQTLDLAAVMPYMWTGLIVGLGAALVSVFLPTWARIAAPVYAAAQGL
                     ALGGISAVYEQQLHGIVFQAIGLTFAVLAAMLVLYRSGIIKVTDRFRMIVFAATGGIA
                     LFYLVSIVLSLFHISMPVVFGGGTLAIAFSVVVVVIAALNLALDFDFIARGAEHGAPK
                     FMEWYAAFGLMVTLIWLYLEILRLLAKLQRR"
     gene            complement(4628..5158)
                     /locus_tag="AC16JC_10790"
     CDS             complement(4628..5158)
                     /locus_tag="AC16JC_10790"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5165223.1"
                     /translation="MKWLVFILAVVVGVPMLMILVGLVLPREHVAGVSVVLRQPAESV
                     WRVVRDLGALPQWWPGLRSSERVTGPDGRERWRQVSGSGALTIVLEEEQPPRRLVSRI
                     ESPPGAPFGGAWIYEIVPEGDGSRVTVTEQGWVANPLFRFLSRFVFGHYRTLESYQRA
                     LGRRFNQGVTPVRQAE"
     gene            5265..5648
                     /locus_tag="AC16JC_10795"
     CDS             5265..5648
                     /locus_tag="AC16JC_10795"
                     /inference="COORDINATES: protein motif:HMM:NF021119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerW family sporulation protein"
                     /protein_id="HIR5165224.1"
                     /translation="MSTKELIDGAVEHLRTSASVKTVYGTPITNDGKTVIPVAKVLYG
                     FGAGSGHGTHAKKSKNGATPAEGEGEGAGGAVAAKPVGVVEITGSETRFVPFGASRKL
                     AIMAVIGAGLGAAFGWFLGRRAGRS"
     gene            complement(5652..6044)
                     /locus_tag="AC16JC_10800"
     CDS             complement(5652..6044)
                     /locus_tag="AC16JC_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006396156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HIR5165225.1"
                     /translation="MTDNAVRLDKWLWAARFFKTRAAAVQAISAGRVEVNGVAAKPAR
                     PLAVGDRLRVRKPPYAFIVKVIALSAQRGPAKVAAGLYEEDPAGKAARERLAHQLRVA
                     PTPSYEGKGRPTKRDRRELERLRGDEAD"
     gene            complement(6083..6436)
                     /locus_tag="AC16JC_10805"
     CDS             complement(6083..6436)
                     /locus_tag="AC16JC_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012374723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5165226.1"
                     /translation="MGNFRKLVVWQHAHRLSLSVYRATIWFPRTEVYGLTSQMRRASV
                     SIGANIAEGCGRNGDRELLRYARIALGSANELEYHVILAKDLEYLPRLASDQLQQDIA
                     VVKRMLAKLVARLDR"
     gene            complement(6564..7613)
                     /locus_tag="AC16JC_10810"
     CDS             complement(6564..7613)
                     /locus_tag="AC16JC_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR5165227.1"
                     /translation="MRSVIVIGLGLAAASPAAAQAGQRYTVAGDNVAIYNLAGVVRVE
                     SGPAGDVVVEVTRGGGDAAKLRVETGPIGTRQTLRVVYPDDDVVYSVMGFHGSTTLDV
                     RDDGTFNGDRRESGRGHRVRISDDGSGLDAHADLRILVPAGRRIAVYLAVGKLTATNV
                     DGEVRLDVSSADVAASGMKGSLTVDAGSGDVRVTDAAGDLNLDTGSGNVVVSGAKGDN
                     LLIDTGSGDVTLDHAQGRTLKIDTGSGNADATAVTADDLIIDTGSGDVTLELLAGGGS
                     IDIDTGSGEVTLTLPGAYGADVILDTGSGEIDLGGLSVTVRRVAEDHIEGRIGDGKGR
                     LHVETGSGDVRLKKA"
BASE COUNT         1150 a         2769 c         2662 g         1267 t
ORIGIN      
        1 ctcacgcact tcgcgttcgt gctcttcgtc atcgcgggcg cgctgctgct gcggcacagc
       61 cgctggtggg ccgtgctgca cgtgccagcg ttcgcgtggg gcgccctgat cgaattccgc
      121 ggctggatct gcccgctgac gccgctggaa aacacgctgc gggcccgcgc cggccaggcg
      181 ggctacgccg gcgggttcgt cgagcattac atcacgcggc tcctctaccc cgaggggctc
      241 acgcgcggca tccaggtcgc cctcggcgcg ctggtgctcg ccggcaacct ggtgttgtat
      301 tggtggctgt ggcgccgcag aggatgagga tgagcggcac ggggacgatt cggcggctcg
      361 cgggtgaggc ggagctgggc gcgtgcgccc gcatgatggc ccagtcggag ccgtggatca
      421 ccctgggacg caccctcgag acgtcgacgg ccaacctgcg tgatcccggg aaggaagtgt
      481 acgtggtgga ggacgagggg cggatctcgg ggttcgtggt catcgacctg cgcggcccct
      541 ttccgggtta catccagacc gtctgcgtca gccccgatgc ccgtggccgc ggacacggca
      601 cgcgtctcat cgcgtgggcc gaagaacgaa tcttccggga cagcccgaac gtgttcatgt
      661 gcgtctccac cttcaatccc gccgcccgcc ggctgtacga gcggctgggc tataccgccg
      721 tgggcgagct gtccgattac atcgtgcccg accacggcga gatcctgctt cgcaaatcgc
      781 gtggcccgtg gtcggagttc cggcgatgag gccggacctg cgcctgggca tcacgatcgc
      841 gatggcggcg accgcccctc cgctcgcggc ccaggattcg gtgccggccc gcgcagacta
      901 cggcgccgcg gccgcggtgc tcacccgctt catcgatcac gaaatggccg acaaggacct
      961 tccggccgtt tccatcgtgc tggtggacga caccgccatc gtgtgggccc gcgggttcgg
     1021 attcgccagg ccgcgggaca gcgtgcccgc cacggcgctg accgtctatc gcgtcggatc
     1081 ggtgtcgaaa ctcttcacgg acatcgcgct gatgcggctc gtcgaccggg ggacggtcga
     1141 tctcgacgcg cccgtcaccc gctggctgcc ggacttccac cccgtcaacc ggttcgagcg
     1201 cccggtcacg ctgcgccagc tcatgaccca tcacgcggga ctggtgcgcg agccgccggt
     1261 ggggagctac ttcgactcga ccgctccgcc cctttcggcg atcgccgcga gcctcaatcg
     1321 gacggcgctc gtataccggc cgggaacccg ctacaaatac tccaacgccg ccctccaggt
     1381 cgtgggctac gtcctggagc gcgcgacgca cgagccgttt ccgcgctacc tgaaggactc
     1441 ggtgttgcag ccgttgggca tgacgcgcag cgcgttcttc gagccggcgc cggaaacggc
     1501 acgcgacctc gccgccgcct tcatgtgggc gcccgacggc cggcgcttcg ccgcccccac
     1561 ctttcggcgc aacgcgccga gcggggcgct gtacaccacc gtggccgacc tcgggcgctt
     1621 tctcgccggc ctcttcgccg actccaatcc cgtcgtgccc cacacgacgc tcgagcgtat
     1681 gtgggccgac agcggcctcg ggttcttcgt ctcgcgcttc gacgggcacc gccgggtggg
     1741 acacgacggc gccatctacg ggttcgcgac gaccctcgcc gccctcccgt acgagaaact
     1801 cggcgtcgcg gtggtcacga ccctggacgg cgccaacacc gtcaccggcc gcgtggccga
     1861 cgccgcgtta cgggtcctgc tggcgcggcg ggccggcgcg ccgctccccg agccgccgat
     1921 gacggcgccg ctcccgcccg gtacggcgcg ggcggccgcg ggacattacg tgcgcggagc
     1981 ccggagcgtg gatctgttga gccgctcggg cgagctgctc gtcgcacgcg acggcagtgc
     2041 cgtaccggcg cgactgcgca ccctgggcga cagcctggtc ttcgacgacg tcctgacctt
     2101 cggcggagcg gtgcgtctgc cggcgggcgc ggtggtcgtg gacggcgaca cgttcgcgcg
     2161 cgcgcccgcg ccccggccgg ccgacgtctc cccgcgctgg aaggggctgc tgggagaata
     2221 cggctgggcg tacgagaccc tgttcgccta cgagcgcgcc ggccgcctgc acgtgctgat
     2281 cgaatggacc gaagacgacg ccatgacccc ggtgtccgat acggtgttcg cgttcccggc
     2341 gacgagcatg tacggggggg agcgcctcgt tttccggggt acgcgatccg ccgggggtcg
     2401 tgcgacgacc gtcatcgccg gggccgtcgc cttcccgcga cgcgtggtcg ggccccaggg
     2461 aggagggcag ctcaaggtga caccgctgca tccggtggcg gagcttctcg cggccgcgcg
     2521 caccgcgaca ccaccggccg agagcgggtc gttccgcgcc ccggagctcg tggatctcgc
     2581 gacgctcgac tccacgatcc acttcgacat ccgttacgcc acgaccaaca attttctggg
     2641 cagcgtcttc tattcgacgg cccacgcgtt cctgcagcgg ccggcggccg aggcgctgtt
     2701 gcgcgccgca cgatcgctgc gcgcgcgggg gtttggcctg ctgattcacg acggttaccg
     2761 gccctggtac gtgacgaaga cgttctggga cgccacgccc gccggactgc gctggctcgt
     2821 cgccgatccg gcccgcggct cgaggcacaa ccggggatgc gccgtggacc tgacgctgta
     2881 cgatctgcgc accggccggc cgatcgacat gggcgggacc tacgacgaga ccaccgagcg
     2941 ctcctacccc gactacccgg tgaccgccga cctggagcgc tggcaccgcg acgagctccg
     3001 gcgggccatg gaggacgagg gattcacgcg cgtgcccaac gaatggtggc acttcgacta
     3061 ccgcgactgg cgcagctacc cgatcctcaa cgttccgttc gaggacctgt gaagaccgct
     3121 ctcatcgccg gcgccaccgg tctggtgggg agccggtgtc tcgcccggtt gctcgcggag
     3181 cccgcctacg aacgggtcat cgcgctggtg cgccggccgc tgccgcacga cgatccccgc
     3241 ctcgaggcgc gggtgatcga tttcggcacg ctcggcgccg ccggcaccgc cttccccgcc
     3301 gccagcgacg tcttctgctg cctcggcacg acgatgaagc aggcgggaac cgaggaggcg
     3361 ttccgcctgg tcgatttcac ctacgtcgtg agtctcgccg cgcaatcgct cgagcacggt
     3421 gcacggcagt tcctcctggt cagctccatc ggcgccaatc gggcatcggc gttcttctat
     3481 cctcgcgtga agggcgagac ggaagacgcg atcgccgcgc tgccgttcga aggaaggcag
     3541 atcttccgtc cgtccatcct cgtgggcgag cgcgccgagc accgccccgg cgagcgcgca
     3601 tggatcgcgg cgatgaaggg gggcgcgttc ctgatgctgg gaccgatgcg caagtaccgc
     3661 ccgatcggcg ccgccgcagt ggccgaggca atggtccgcg tggcgctgcg ggcgccgcgc
     3721 ggcgtcaacg tctatgaatc caacgagatc gcgcgcatcg cccgcggctg acccaccccg
     3781 ttatattgcg gtgcacattc gactcctgaa ggacgtgagc atggccaacc cattattgag
     3841 cgacaaggca ttccagcgcg cgggccagta cgcgcaggcg accaccggcg agttgatgac
     3901 ggtcgaaggg gcgatcaaca agtcggcgtt cctgctgctg ctggtggtcg gtgcgggcac
     3961 ctgggtgtgg acccgctact accagacgct cgacctcgcg gcggtgatgc cctacatgtg
     4021 gaccggcctg atcgtggggc tgggtgccgc gctcgtgagc gtgttcctgc cgacctgggc
     4081 gcgaatcgcc gcgcccgtct acgccgccgc gcagggcctc gcgctgggcg ggatctcggc
     4141 ggtgtacgag cagcagctgc acggcatcgt cttccaggcg atcggcctca cgttcgccgt
     4201 gctggccgcg atgctggtgc tgtaccgctc gggcatcatc aaggtgacgg accggttccg
     4261 catgatcgtg ttcgcggcga cgggcggcat cgcgctgttc tacctcgtca gcatcgtgct
     4321 gtcgctcttc cacatctcca tgcccgtggt gttcgggggc gggacgctcg caatcgcgtt
     4381 cagcgtggtc gtcgtggtga tcgccgccct gaacctggcg ctggatttcg acttcatcgc
     4441 ccgcggcgcc gaacacggcg cgcccaagtt catggagtgg tacgccgcct tcggcctgat
     4501 ggtgacgctg atctggctct acctcgagat cctgcgcctg ctcgccaagc tgcagcggcg
     4561 ctgagccgaa ccttcacgtt tcgtcgcgaa cggcgggccc acggtccgcc gttcgtctgt
     4621 tcacgaccta ctccgcctgg cgcacgggtg tgaccccctg gttgaaccgg cgccccagcg
     4681 cccgctggta actctccagc gtccggtaat ggccgaacac gaagcgcgat aggaaccgaa
     4741 acagcggatt ggcgacccag ccctgctcgg tgacggtcac gcgactgccg tcaccctccg
     4801 gaacgatctc gtagatccag gcgccgccga acggcgcgcc gggcggcgac tcgatccggc
     4861 tcacgaggcg gcgcggcggc tgctcttctt cgaggacgat cgtcaaggcc ccgctaccgg
     4921 acacctgccg ccagcgctcg cggccgtccg ggcccgtcac ccgctccgac gagcgcagcc
     4981 cgggccacca ctgcggcagg gcgccgaggt cgcgcaccac cctccacacg ctctccgccg
     5041 gttgccgcaa cacgacggac acgcccgcca cgtgctcacg cggcagcacc agaccgacca
     5101 ggatcatcag catcgggacg ccgaccacga ccgcgagaat gaacaccagc catttcatgg
     5161 ggtctcctcc atggggggcc aacttcgagg agaatgccct cctcaccggc cgttcacaac
     5221 accttcaatc gtcgcggggt aggctgccct gcaggaggcg agatatgagc acgaaagaac
     5281 tgatcgatgg cgcggtcgag catctgcgga ccagcgcgag cgtgaaaacc gtgtacggca
     5341 ctccgatcac gaacgacggc aagacggtca ttccggtcgc caaggtgctg tacgggttcg
     5401 gggcgggcag cggccacggc acccacgcca agaagagcaa gaacggcgcg acgccggcgg
     5461 agggagaggg cgaaggagcg gggggcgccg tcgccgcgaa gccggtgggg gtcgtcgaga
     5521 tcacgggcag tgagacccgg ttcgtgccgt tcggcgcctc gcggaagctc gcgatcatgg
     5581 cggtgatcgg cgccgggctg ggcgccgcgt tcggctggtt cctggggcgg agggcaggca
     5641 ggtcctgagg actagtcggc ctcgtctccc cgcagccgct ccagctcccg ccgatcgcgt
     5701 ttggtgggcc gcccttttcc ttcgtaggac ggcgtggggg cgacgcgtag ctggtgcgcg
     5761 agtcgctccc gcgccgcctt ccccgcggga tcctcttcgt agagaccggc ggcgaccttc
     5821 gcgggaccgc gctgggcgct gagggcgatc acctttacga tgaacgcgta gggcggcttg
     5881 cgtacccgca gccggtctcc gacggcgagc ggccgcgcgg gcttggccgc gacgccgttg
     5941 acctcgaccc gacccgcgct gatcgcctga acagccgcgg cgcgggtctt gaagaagcgc
     6001 gcggcccaga gccatttgtc gagacggact gcgttgtcgg tcattggctg ttggctatcg
     6061 gctatcagct atcggctatc ggctatcggt cgagacgagc tacgagcttc gccagcatcc
     6121 gcttgacgac cgcaatgtcc tgctgcaact gatcgctcgc aagtcgtggc aggtactcca
     6181 ggtctttcgc caagatcaca tggtattcga gttcgttcgc cgatccgagg gcaatccggg
     6241 cgtaccgcag caactctcgg tctccatttc gaccgcagcc ttcggcgatg ttggcaccaa
     6301 tagatacgga cgcacggcgc atctgtgacg tcaaaccata gacctcagtt cttggaaacc
     6361 aaatcgtggc gcggtagacc gataggctca agcggtgggc atgctgccaa accaccagtt
     6421 tccggaagtt ccccatgcca acaggatcac acgatgcaga ccactggata caaccgtttc
     6481 gccccgcggg ccatcggaaa aacgcgacca ccgatcctgg accggtggtc gcctgtgccg
     6541 acagccgata gccgatagcg tgtctaggcc tttttcagtc tcacatcccc actccccgtc
     6601 tccacatgca gccgcccctt gccgtcgccg atgcgcccct cgatgtgatc ctccgccacc
     6661 cgccgcaccg tgacggagag gccgccgaga tcgatctcgc ccgagcccgt atcgaggatc
     6721 acgtcggccc cgtaggcgcc cggcagggtc agcgtgacct cgcccgaacc ggtgtcgatg
     6781 tcgatcgagc cgccgcccgc cagcagctcg agcgtgacgt cccccgaccc cgtgtcgatg
     6841 atgagatcgt cggcggtcac ggcggtcgca tcggcgttac ccgacccggt gtcgatcttg
     6901 agggtgcggc cctgtgcgtg atccagcgtg acgtcgcccg accccgtgtc aatcaacagg
     6961 ttgtcgcctt tcgccccgga caccaccacg ttgccggagc cggtgtcgag gttgaggtcc
     7021 cccgcggcat cggtcacacg cacgtcgccc gagccggcat ccacggtgag cgagcccttc
     7081 atgccgctcg ccgcgacgtc ggccgagctg acgtcgagcc gcacctcgcc gtccacattg
     7141 gtcgccgtca gcttgccgac cgcgaggtag accgcgatgc ggcggccggc gggaaccagg
     7201 atgcggaggt cggcgtgggc gtccaggccg gacccgtcgt cgctgatgcg cacgcgatgg
     7261 ccgcgcccgg actcccggcg atcaccgttg aaggtgccgt cgtcgcggac gtccagcgtg
     7321 gtgctcccat ggaatcccat gacggaatag acgacgtcgt cgtcgggata gaccacgcgc
     7381 aacgtctggc gcgtgccgat cggccccgtc tcgacccgga gcttggcggc gtcgccgccg
     7441 ccgcgggtga cttcgaccac cacgtccccc gcgggtccgc tctccacccg cacgacgcct
     7501 gccaggttgt agatcgcgac gttgtctccg gccacggtgt agcgctggcc ggcctgggcg
     7561 gccgcggggg acgcggcggc aagtcccaag ccgatgacga tcacgctgcg cattgcaact
     7621 ctcctcggga gtgtgaagac gaccggtggc cttacgccgc ttccgggtcg cggtttcgcg
     7681 acgatcgtct gcacggagtg gatagacaga aggcccacag ggttgcagcg gtcgcgcggc
     7741 gccccccggg gcggtagttt ccacccgcgt tggcgaccct gacggcggag gatccgggtg
     7801 tcgagctggc agatctggat cgtggcggcg gcgctgctgc tcgtggcc
//
LOCUS       DBNGZW010000458         2070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_794779, whole genome shotgun sequence.
ACCESSION   DBNGZW010000458 DBNGZW010000000
VERSION     DBNGZW010000458.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_794779"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..1617
                     /locus_tag="AC16JC_10815"
     CDS             88..1617
                     /locus_tag="AC16JC_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009744779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1593 domain-containing protein"
                     /protein_id="HIR5165228.1"
                     /translation="MRNVTDAPVRPKPRVVVTTDPELDDANSLIRYLLYSTDFRTEGL
                     VYASSGYHWKGDGTGKKVSVPNREYSRWGLNLCPCASWRWAPDEHYIDVAVATYAQVY
                     PSLRRHDPGYPTPAELKSKIRWGNVEFDGDISKDTPGSNLIKALLLDDEPGPVYLLAW
                     GGQSTIARALKSIQEEYARAPQWPAIYQKVSRNAILWTSGDQDDTYATYIHPNWPGIR
                     SLPGGVGGVPLAYGAQATASAEDSVYYSAEWTRANISSRGPFGALYRVWGDGKQMVKG
                     DIFDYFGLSGYTADQLKAMGYIVWTPPRPAGEFLGEGDTFTFLNLIDNGLRGYEDETP
                     GGWAGHGPVGTAAAPGFGPGITINSYDDLLRLQERAALAGRPPSPDPDFTPAAQNGLA
                     ARLLWSVTPTYGDANHEPTVSVRGNSRIFARPGETVRLEAVASDPDGNAVTVRWRQWK
                     AVGTYPGRVTLSNPAALATRLQVPADATAGQTIHLILEATDNGTPPLTRYQRVIIGVR
                     R"
     gene            1642..>2070
                     /locus_tag="AC16JC_10820"
     CDS             1642..>2070
                     /locus_tag="AC16JC_10820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165229.1"
                     /translation="MIRAALVMALVAAAPMERLAAQCPDGSAPPCRAARPAPATNSVA
                     VLYFENRSRDSADAYLAEGLTEAIITQLGDLPRLTVKSRFLVRRYRGMAAPADPAAIG
                     RALGATYLVTGSVQRAGARLRVTAELARASSGDRVWGQQYD"
BASE COUNT          341 a          715 c          700 g          314 t
ORIGIN      
        1 aacatgaggc ggctttcgat cccccggcgc ttccagccgc tgcttctcac ggtgacccta
       61 ggcggctgcg cgacgagcac tccccccgtg aggaatgtca cagacgctcc agtcaggccg
      121 aagccccgtg tcgtcgtcac cacggacccc gagctcgacg acgccaattc cctcatccgc
      181 tatctgctct acagcaccga tttccggact gaaggactcg tctacgcctc gagcggctac
      241 cactggaagg gcgacggcac cgggaagaag gtgtcggtcc cgaaccggga gtattcgcgg
      301 tggggcctca acctctgtcc ctgcgcgtcg tggcgctggg ccccggacga gcactacatc
      361 gacgtcgccg tggccaccta tgcccaggtg tatccgagcc tgcggcggca cgatccggga
      421 taccccacgc ccgccgagct caagtcgaag atccgatggg ggaacgtgga gttcgacggg
      481 gacatctcga aggacacacc tggctccaac ctgatcaaag cgctgctcct ggacgacgag
      541 cccggcccgg tctacctcct cgcgtggggc gggcagagca cgatcgcgcg ggcgctgaaa
      601 tcgatccagg aggagtacgc gcgggcgccg cagtggcctg ccatctatca gaaggtctcg
      661 cgcaacgcca ttctctggac ctccggggac caggacgaca cctacgcgac gtacatccat
      721 cccaactggc ccggcatcag gtcacttccg gggggcgtgg gcggcgttcc gctggcctat
      781 ggggcccaag ctacggcgag cgccgaggac tcggtctatt acagcgcgga gtggacgagg
      841 gcgaacatct cgagccgcgg accgttcggc gccctgtacc gcgtgtgggg cgacgggaag
      901 cagatggtga aaggcgacat cttcgactac ttcggactgt cgggatacac ggctgaccaa
      961 ctcaaggcga tgggctacat cgtttggacg ccaccacgac ccgcgggcga gttcctcggc
     1021 gagggcgaca cgttcacctt cttgaacctg atcgacaacg gactgcgggg atacgaagac
     1081 gagacgccgg gcggctgggc ggggcatggc ccggtcggca cggccgccgc tccgggcttt
     1141 gggccgggca ttacgatcaa ctcgtacgac gatttgctga gactccagga gcgggccgcg
     1201 ttggccggac ggccaccgtc accggacccg gacttcacgc cggccgccca gaacgggctt
     1261 gccgcgcgcc tcctgtggtc ggtgaccccg acgtacggag acgccaacca tgagcccacc
     1321 gtttcggttc gcgggaactc gcggatcttc gctcggccgg gtgagacggt tcgcctcgag
     1381 gccgtagcgt ccgatcccga tgggaacgcg gtgaccgtgc gatggcggca gtggaaagcg
     1441 gtgggcacct atccgggccg ggttacgctc tcgaaccccg cggcgctcgc gacgcgcctg
     1501 caggtaccgg ccgacgcgac cgcgggacag acgatccacc ttatcctcga ggcgaccgac
     1561 aatgggacgc cgccgcttac acgctatcag cgggtgatca tcggtgtccg acgatagcca
     1621 gggcaaggtg atggtggcac ggtgatccgc gcggcgctcg tcatggctct cgtggccgcc
     1681 gctccgatgg agcgcctggc cgcacagtgc cccgacggct cggcgccgcc gtgccgcgcc
     1741 gcgcgtcccg cgcccgcgac gaacagcgtc gcggtcctct atttcgagaa tcgctcgcgc
     1801 gacagcgccg atgcctacct ggccgagggc ctgaccgaag cgatcatcac gcagctcggc
     1861 gacctgccgc ggctcacggt gaagtcccgg ttcctggtac gtcgttaccg cggcatggcg
     1921 gcacctgccg acccggccgc gatcggccgc gccctcgggg cgacctacct cgtgacgggc
     1981 agtgtccagc gcgccggtgc ccgcctgcgc gtgaccgcgg agctggcgcg ggcctcgagc
     2041 ggcgatcgcg tctggggtca gcagtacgac
//
LOCUS       DBNGZW010000459        12095 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_928657, whole genome shotgun sequence.
ACCESSION   DBNGZW010000459 DBNGZW010000000
VERSION     DBNGZW010000459.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 12095)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12095)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12095
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_928657"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3241
                     /locus_tag="AC16JC_10825"
     CDS             <1..3241
                     /locus_tag="AC16JC_10825"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5165230.1"
                     /translation="AVTLAVGDTPGGGTLTGSVTVAAVGGVATFPDLTIDKSGSGYSL
                     VASAAGAATGASARFDVTAGTAAQLAFIVQPSSPFQGTAIAPAVQVGAQDAFGNAATG
                     FAGDVTVAIGTNPSSGTLSGTTTTAAVAGVATFADLRIDSSGTGYTLTATAAALTSAA
                     STAFEVIPVERFTRLAFTAQPTDVAAGSAIAPAVTVTALDQLGNPATAFAGSVTITLA
                     ADPAAGILSGTTSATAVSGVARFDDLRLDRLGTGYRLQAAAAGLAPDTSNAFSVRAGP
                     PARITVQDGNGQTAPAGTTLPIPYLVDVRDAFDNPVRGTTVSWSVTGGGGAVNPAQSS
                     TDSTGHASGVRTLGLHAGTHTAAAAVAGLADSVASFSATATPNGTISGTITTTSGYLA
                     SPAPVSAPKDAPLLAYTSDELIVTYRAGALGSPPVGSAALAAPATVAGVGSTIRGRLG
                     ALPAAAQFRVRGVSPVLLAARVQVHDSLQRDAIAAALRRDAAVESVERNGIVHLERRR
                     SAAAATVTLPGDPLYPRQAWHYGLIDLPRAWAITTGSAAVLVAVVDNGIRYDHPAVAG
                     NLTHDGYDFVSSASYKLCSGGGTIDNAGDGDGYDADPTQPADYDINFSRSCAIGLTAS
                     GNHGLHVAGTIGAVGNDGVGVSGVNWTVRIRPVRVLGVAGFGTFYDIAQGILYAAGLP
                     ADDGKSGTVTAPSGARIINVSLGGPSGSTALHDAVISATNAGALLIAAAGNSGNTSLL
                     YPAAYPEALSVSAVGPDALLASYSSYGSTVDIAAPGGDQADGDASFGVLSSAWNYVAT
                     TPIYDSWDGTSMATPHVSGVAALLLAQDPSLMGAQLRARLTTYAVDAGAPGPDDQYGA
                     GIVNARNSLTQSLAPPRATYARLYTADGLLVATTRTAADGSYAFTGLGDASYTVYGGT
                     DESGDGQVGVPGRLWGALGGSATPVAVTVDGAADYAATFAIALPEEIEPNDTPGQANA
                     LALGGYVNAAIGSASDVDVFRVRVPATGTYTVETSAVNGACGFALEANTVLTLRDSGG
                     TVLASNDDIDAGSLNYCSRISTALTPGTYDVTVSSGATSRYRLAVRPGN"
     gene            complement(4962..5198)
                     /locus_tag="AC16JC_10830"
     CDS             complement(4962..5198)
                     /locus_tag="AC16JC_10830"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5165231.1"
                     /translation="MRLTAREEEIARLVALGHTDKEVARRLGISLSTARCHLRNIRDK
                     LGIHSRVGIALHMWERRPSDGTANHDGVGRGPPH"
     gene            complement(6217..9363)
                     /locus_tag="AC16JC_10835"
     CDS             complement(6217..9363)
                     /locus_tag="AC16JC_10835"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5165232.1"
                     /translation="MADLRNRLEAALAGRYTIDREIGRGGMAIVFLAYDLRNERDVAL
                     KVLRPELAVTLGPERFLQEIRFSANLAHPHILPLHDSGDADGLLYYVMPFVRGESLRD
                     RLEREGQLPLVDALTIAREVADALDYAHRAGVVHRDIKPENILLQEEHAVVSDFGIAR
                     AISAAGARVTAVGTTVGTPDYMSPEQGEGKGPLDGRSDIYSLGCVLFEMLAGLPPAAL
                     TPPEGPAAGPLRARNRLAELTMLRPSVPPEVAGIVARMLEPKAKDRFATGAEAAEALA
                     APSGIWTPRSVLARRRRRWGAGVAGAVCVGVAGFVVIPRILGAGLDQSVVLVVPFTPR
                     DSTPTGLLDGGGIERLLSDAFSRWTDIHIVGDLELADVLRRAGDRNPSLQASLTYARA
                     HRAGRLVWGEYGAVGDSIEIVAGLYDVADGNAVARALRRVAANGAGLPQQIQELAEEL
                     LVNRAPPAAKSGFMGTTLVSALQAYLDGHAAIKDWNLDSAVTAFRTALARDPFFPQAN
                     LWLAQTLEWTGADATLWQRAAERAAAAGDTLNARERLLAAGLVGLASGQFPHACGRYQ
                     EVVARDSLDFAGWFGLGECRAKDDAVVRDAASPSGWRFRSSYHAALRAYSRALRLVPS
                     AQRAFRLSQPRRLIFYTEANRFRPGFALDPDTVWYAAFPTLDHDTLAFVPYLRSVTLG
                     RPQPTSTPAAVAYNQGVLREMTSAWVRAFPADPAACEAHAQVLESMEDLDGRLEENSA
                     LAMTRRARRFASDSIQALRLAVAEARLLTKLEDFAGARRLADSILNAWSKGPPGTAVA
                     LAGLAALTGRVHLTARLLAEAGPDTMLESGGEELRVRPLSLARAVFALHAYAAFGTPS
                     DSIVGLARRVERLVDASVEAQRRESLRRFLLDKPTVWAFPLLGASVPERGHPGETRRR
                     MLWALMHGDTGVVRAALDTLNAVWAALRPGDVAITGTFVQSRVLLAIGDSVGAEQLLG
                     RSLDALSTQGSGLLDNVDQAASLVRAMALRAELADREGDAATGRRWGAAVVHLWSGSD
                     DASLREVVQRMRRLAAQN"
     gene            9561..10514
                     /locus_tag="AC16JC_10840"
     CDS             9561..10514
                     /locus_tag="AC16JC_10840"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012282347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR5165233.1"
                     /translation="MADYTFARGQLLLLAGTANRPLAEEMAQHLNQPLCAVTIRHFAD
                     GELFVKIDENVRGRDVFIIQPTNPPAENYLELLLLIDAARRASAARITAVLPYFGYAR
                     QDRKDQPRVAVSAKLMANLITHAGADRVLGMDFHSHQLQGFWDIPVDHLYAAPVFVSH
                     FRTKGLREPVVVAPDVGSAKMARGYAKRLNASLAIIDKRRPSANVAEVVNVVGEVEGM
                     DCLIPDDMIDTAGTITEAAAALKRLGAQRIFAFATHALLSGPAVERLRSSPIEEVTVT
                     NTIRLPEDRKFEKLKILSIAGLMAKAIGYEHSNQSVSSLFD"
     gene            10549..11289
                     /locus_tag="AC16JC_10845"
     CDS             10549..11289
                     /locus_tag="AC16JC_10845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR5165234.1"
                     /translation="MAQFVTLATSPRDKTGKGAARQIRFRKKVPAVIYGHGRPSQPLE
                     LDAKALELALIGIEPASTIIEIELEGKKSRTLIREIQRHPVRPDIIHVDFYEVRAGEK
                     VTLQIPIRLTGTADGVRNAGGVLDQVMREVEIEVLPEHIPDRVELDVTPLLIGHSLHV
                     RDLVIPNATILTQADLTIATVVPPRAEEVAAPTPEAAAEVAEPELIRKVREEEGEEGA
                     EGEAAGEAKGKAAAPEAKAEKPKGGKEG"
     gene            11295..11837
                     /gene="pth"
                     /locus_tag="AC16JC_10850"
     CDS             11295..11837
                     /gene="pth"
                     /locus_tag="AC16JC_10850"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR5165235.1"
                     /translation="MRAVVALGNPGPEYAATRHNVGFWVADALVAHWGFPPFRRGERA
                     RVSEGQVDGTTVRLLKPTTMMNRSGAALAGLRADPGFVPATDLLVVVDDFNIPLGTHR
                     LRASGSAGGHNGLKSIEGALQSQDYARLRIGVGPVPPGEPWEDFVLSPFADEEREQME
                     ALLPQLVAAVAGWVRQGVVS"
     gene            complement(11830..>12095)
                     /locus_tag="AC16JC_10855"
     CDS             complement(11830..>12095)
                     /locus_tag="AC16JC_10855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR5165236.1"
                     /translation="GKLELEVVTPGEGGISETDLLVHDETNRTLATLLAALQPPAFPM
                     ALGVLYCDPTTSYEREVEAQMAAAGSRGELAELLRKGRTWQVS"
BASE COUNT         2032 a         4240 c         3972 g         1851 t
ORIGIN      
        1 agcggtgacg ctcgcggtcg gcgacacccc cggcggtggc acgctgaccg gaagcgtcac
       61 cgtcgcggcc gtcggcgggg tcgcgacgtt cccggacctg acgatcgaca agagcggctc
      121 gggctactcg ctggtggcga gcgcggccgg cgcggcgacc ggcgccagcg cgcgcttcga
      181 cgtgacggcg ggaaccgccg cgcagctggc gttcatcgtc cagccgagca gcccgttcca
      241 gggcacggcg atcgccccgg cggttcaggt gggcgcgcag gacgccttcg gcaacgcggc
      301 gacgggtttc gccggcgatg tcacggtcgc catcgggacc aacccgtcga gcgggacact
      361 gtctgggacg acgaccacag cggccgtcgc gggtgtcgcc acgttcgccg atctccgcat
      421 cgattcatcc ggaaccggat acacgctcac cgccacggcc gccgctctga cgtccgccgc
      481 ctcgacagcg ttcgaggtca tccccgtgga gcggttcacg aggctggcct tcaccgcaca
      541 gccgacggac gtcgcggcgg ggagcgcgat cgccccggct gtgacggtca cggccctgga
      601 ccagctgggc aatcccgcca cggcgttcgc cgggagcgtg acgatcacgc ttgcggccga
      661 tcccgcggcg ggcatcctca gcggcaccac gagcgccacc gccgtgtccg gcgtcgcccg
      721 gttcgacgac ctgcgcctgg acagactcgg cacgggctat cgcctgcagg cggccgcggc
      781 ggggctcgcc cccgatacga gtaatgcgtt cagcgtccgc gccggtccgc cggcgcgcat
      841 cacggtccag gatggcaatg gacagaccgc tccggcgggg accaccctgc cgattccgta
      901 cctcgtggac gtgcgcgacg cgttcgacaa cccggtgcgc ggtacgacgg tgagctggtc
      961 ggtgaccgga ggcggcggtg ccgtgaaccc ggcgcagagc agcaccgact ccaccggaca
     1021 cgcgagcggc gtccgaacgc tcggcctcca tgccggaacg cacaccgccg cggccgccgt
     1081 cgccggcctg gccgattccg tcgcgtcgtt cagtgcgacc gcgaccccga acggaacgat
     1141 cagcggaacg atcaccacga cgagcggata cctcgcgtct cccgctcccg tttctgcacc
     1201 gaaggacgcg ccgctgctcg cgtatacctc ggacgagctg atcgtcacct atcgcgctgg
     1261 ggcgctcggg tcgccgccgg tcggctcggc cgcgctcgcg gctccggcca cggttgcagg
     1321 ggtgggaagc acgatccgcg ggcggctggg cgccctcccg gcggcggcgc agttccgcgt
     1381 tcgtggcgtg tcccccgtcc tccttgcggc gcgggtgcag gtgcacgatt cgctgcagcg
     1441 cgacgcgatc gccgccgcgc tgcgccgcga tgccgccgtc gagtcggtgg agcgcaacgg
     1501 catcgtgcac ctggagagac ggcgctccgc cgcggcagcg acggtcacgc tgcccggaga
     1561 cccgttgtac ccgcggcagg cgtggcacta cggcctgatc gatctgcccc gcgcctgggc
     1621 gatcactacc gggagcgccg ccgtgctggt cgcggtggtg gacaacggca ttcgctacga
     1681 ccaccctgcg gtggccggca acctgacgca cgacgggtac gacttcgtgt cctcggcgag
     1741 ctacaagctg tgcagcggcg gcggcacgat cgacaacgcc ggcgacggcg acggctacga
     1801 cgccgatccc acgcagccgg cggactacga catcaacttc tcgcggagct gtgccatcgg
     1861 actcaccgcc tcggggaacc acggcctgca cgtggcgggc acgatcggcg ccgtgggcaa
     1921 cgacggcgtc ggcgtcagcg gcgtcaactg gaccgtgcgc atccggccgg tgcgcgtgct
     1981 cggcgtggcg gggttcggga ccttctacga catcgcgcag gggatcctct acgccgccgg
     2041 cctccccgcc gacgacggca agagcggcac cgtcaccgcg cccagtggtg cacggatcat
     2101 caacgtgagt ctgggtggcc cgtcgggcag caccgcgctg cacgacgcgg tgatctccgc
     2161 caccaacgcc ggcgcgctcc tcatcgccgc ggccggcaac agcggcaaca cgagcctgct
     2221 ttatcctgcg gcgtatcccg aggcgctctc ggtctcggcc gtcgggccgg acgcactgct
     2281 ggcctcgtat tcttcttatg gttcgacggt ggacatcgca gcgcccggcg gcgaccaggc
     2341 cgacggcgac gcgagcttcg gtgtgctgtc gtcggcgtgg aactacgtcg ccactacacc
     2401 gatctacgat tcgtgggacg gcacctcgat ggcgacgccg cacgtctccg gtgtggcggc
     2461 gctgctgctg gcgcaggatc cgtccctgat gggagcccaa ctacgcgcgc gactcacgac
     2521 gtacgcggtc gatgcaggcg cgccgggacc cgacgaccag tacggggccg ggatcgtgaa
     2581 cgcgcgcaac agcctgacgc agtcgctcgc gcccccgcgc gcgacctacg cgcggctgta
     2641 cactgcggac ggcctgttgg tcgccacgac ccgcacggcg gcggacggct cctacgcgtt
     2701 caccggcctg ggagacgcga gctacacggt gtacggcggg acggacgaga gcggagacgg
     2761 ccaggtgggg gtgcccggtc gcctgtgggg cgccctcggc ggctcggcga cgccggtcgc
     2821 ggtgacggtg gatggggcgg ccgattatgc cgccacgttc gccatcgccc tgccggagga
     2881 gatcgagccg aacgacacgc ccggccaggc caatgccctt gcgctgggcg gctacgtgaa
     2941 tgcggcgatc ggatcagcgt ccgatgtgga tgtgttccgc gtgcgcgttc cggcgaccgg
     3001 gacgtacacc gtcgagacgt cagccgtgaa cggcgcctgc ggattcgcgc tcgaggcgaa
     3061 caccgtgctc acgctcagag actcgggcgg gacggtgctc gcctcgaacg atgatatcga
     3121 cgctggatcg ctgaactact gctcccggat cagcacggca ctcacgcccg ggacgtacga
     3181 cgtcacggtc tcgagcggcg ccaccagccg ttaccggctc gcggtgcggc cggggaattg
     3241 acccccgctg gctgtcttca ctgagctcgg accggcaaca accgacagcc tcgcgggacg
     3301 tgcggcacag gagttgatcg ggcggcgggc gcttacagca cctgcttgca gcacacgcca
     3361 ttgcaggacc ccccatgcca gctcatcatt ggtagtccac acgaagcgtg cgagaggttg
     3421 attgacccgg caccgaccgg ggtgattccg catggttttc agcccgctga cggtacgccc
     3481 gatgttgttg ccgcccgagt ccacggtgac gaagaccgag cgaccgctgt cggtacgcac
     3541 gttgtactcg acccacctcc gcggtgaacg gggcgtgcgg cctggcgctc gaggcgaaca
     3601 ccgtgctcac gcttcgtgat tcgcgcggga cggtgatcgc ctcgtacgac gacattgacg
     3661 ccggggcggt caaatattgc cccccatcag cgtagtgctc gcctccggga cctactacgt
     3721 cacagtgtcg ggccgcacag catgtcggta tctaatcgcc gtccgaaaag gcaattgaca
     3781 atgctttttg gcctcacgaa aatggccacg tttcgctgta ctcccgcccc caatgtgcaa
     3841 gcgtaggtag agccaattgc cccggaccgc aattcgttca gggcttcttc cctcccgacg
     3901 acgggcagac caggcaacac ccgtgagtac aagcgacgtt ggcgcactgg ccgtttcctc
     3961 gagtcagcca gcgcgccatt gggaagttca ttgtagccca gctgctactc aaccattcga
     4021 tccgctgtgg caggattcgc acttgatccc tttcgcgctc agatatgatg attgctcgcg
     4081 cagttcccga tgtatcacta gttgacagaa gactgtcgac ccagatgtag ctaattccag
     4141 gtggcagctg caaggcgggc cagcccctac cctctgtggg gtcgatccag atccgcgcca
     4201 cgattctccc gtttccagga ccggtgcctt caagattcgc aagccggtta gagtgggcat
     4261 tgacttgaga tgccactcgt gccgcgactg accgcgtaat gctccgcccc gaggaatctg
     4321 tctcggtcag tattgtcgag tctgcagtac cgtgcgtgac actgtcatat gccaggctac
     4381 gcgcgtaggc gagaagaaga tcacgactct caggattgac aggatcatac ttgaacgccg
     4441 gtgccggcgg catgttgtga gccttgggta caagccagcc agctgcggca acgacgagaa
     4501 gggtggcaat cgagatgaag aagacagcac gaatcgttcg tattcctcgc tcggtcataa
     4561 cgccccctga gtggtaacat ggaaggacct atggctccgc gtagcctaca cattctcgac
     4621 gctcgaaccg gtggccccca gaagcacccc ttccattgag tcgcgacgat cgacgatctc
     4681 agctgagcag agccctggca agtaggctca ggcccacgga actcgctatc ccgggcctgc
     4741 ggcacccgga cggcgtctac gcgacccacc cctcatcacc cttcggagtg aacaagcacc
     4801 gcgccagcgt cggccccgtg atccccgagc tgttgcggcg attctcgata tggcgattct
     4861 cgttataggg taagcgacgc acgaatggat gggctccaga ctcgggcatc acaacgtctc
     4921 ggctcaatct ggaggctagt tccacgccgc cacaatcgtc attagtgagg aggaccgcgt
     4981 cctacgccat cgtgattggc cgttccgtcg gagggtcggc gttcccacat atgcagcgcg
     5041 ataccgacgc gcgaatggat cccgagctta tcccgaatat tgcgcaggtg acacctcgcg
     5101 gtgctaaggg agatgcccag ccttctggcc acttccttgt ccgtatgccc cagggcaacg
     5161 aggcgcgcga tctcttcctc tctggcggtc agtctcattg cgccaccctc cgcaacgaag
     5221 ccgctcagct atagtcgccg ttcttcatgc aagacagagg cagcacgaca ggctgcgtcc
     5281 ccgtctcgga aaatccgaca taatgatggc cctgacgaca ccggaatcgg gtgagggccg
     5341 aaggtcaggt cggactggaa caccattcgg agcccttgca ggtccagcat tgacccggcg
     5401 tccaccgcgc tactgcctgg ttccacgtcg taccgtggta gaggcggact ggcttcgcgc
     5461 gtgcggggag gctcccattc gcaggcacga ccaccgcccg ggcggtgctc ccttcggcag
     5521 tggaggtaag gctatccacc cacaagtagc tgacgcctgg cggaacgccc agcgattcgt
     5581 agacccgcag actatcgaaa tccggccgag cgagacggat ttcgattctc agctggacgc
     5641 gtccgcggcg aaggtcggtg ccatgggagc ggtggatgtt cacctccggc gagacgatgg
     5701 cgatcgtccc cagagtatct acgatggcaa agtcacactg gccatgatac tcgtttgcgg
     5761 agtcgtgttg cgtgtgcgat tcgcagacag tgtccgcgta ggtcaagccg cgagcgtacg
     5821 ccagcaccgc cccgcgctgc gcggggtcga gcgggttcgt aacagaatcc ggcggagggt
     5881 cggtccggct tccgagagtc cctaatgcga accccgtgag gagccacgcg atacccgctg
     5941 ccgagccagc ggtcacaacc gcgagcgctg tccaaggcac gaggcccatg ggcgccggcc
     6001 tgggccgtgt aagccacacg gcgacaccaa gcaggaccac gaacaccacg atggcagcga
     6061 gtggctcgat gaacagaaga atgacaccca cgacagtcaa caacgatacc gcgatcagcc
     6121 accccgtcgc ccctggcgac cccgccggac ccgacgtccc cgtcatatgt ccccccggtc
     6181 atgttgagac tgccgacaac gctccagtga aaagggctag ttctgggccg cgagcctccg
     6241 catgcgctgg acaacttccc gcagcgacgc gtcgtctgat ccgctccaca gatgcaccac
     6301 ggcggccccc cagcgacgcc cggtcgcagc gtcgccctct cgatcagcaa gctcggcgcg
     6361 cagcgccata gcacgcacta gactggccgc ctggtccacg ttgtccagca accccgagcc
     6421 ctgcgtggac aatgcgtcga gtgatcggcc caggagctgc tcggcgccca ccgaatcacc
     6481 gatcgccagc agcacgcgac tctgcacgaa tgttcccgtg attgccacgt ctccgggacg
     6541 caaagcagcc catacagcgt tcaatgtgtc caatgctgcc cgcaccacgc ccgtgtcccc
     6601 gtgcatgagc gcccagagca tccgccgcct ggtctcgccg ggatgcccac gctcggggac
     6661 agatgcgccg agcagcggga acgcccacac cgtcggcttg tcgaggagga atcgccgcag
     6721 ggattcccgg cgctgagcct cgacggacgc gtcgacgagt cgctccacgc gacgcgcaag
     6781 cccgacgata ctgtcgctcg gggtcccgaa ggccgcgtaa gcgtggaggg cgaacaccgc
     6841 acgggctagc gatagcgggc gcacccgcag ttcttcgcct ccgctctcga gcatcgtatc
     6901 aggcccagct tcagccaaga gccgcgcggt gaggtgaacc cggccggtta gagccgctag
     6961 gccggcgagc gccaccgcgg ttcccggcgg acctttggac caggcgttga ggatggagtc
     7021 ggccagtcga cgcgcccccg cgaagtcctc gagtttcgtg agcagacggg cttcagccac
     7081 ggcgaggcga agcgcctgga tcgagtccga ggcgaagcgc cgagcgcgtc gggtcatggc
     7141 gagcgccgaa ttctcttcca gccgaccatc caaatcctcc atgctctcca gaacctgcgc
     7201 gtgcgcctcg cacgctgcgg gatcagcggg gaaagcgcgt acccaagctg atgtcatttc
     7261 gcgcagcacg ccctggttgt aggccacggc cgccggcgtc gacgtcggct gaggcctccc
     7321 cagagtcacc gagcgcaggt acgggacgaa cgccaaggtg tcgtgatcca gcgtggggaa
     7381 ggcggcatac catacggtgt ctggatcgag cgcgaacccg ggccggaacc gattcgcctc
     7441 agtgtagaag atcagtcgcc gaggttggga cagacggaac gcccgctggg cggatgggac
     7501 aagtcgcagt gcgcgactgt acgcccgtag ggcggcgtgg tagctgctcc ggaatcgcca
     7561 acctgagggg ctcgcggcgt cgcggacaac cgcatcgtcc ttggcccggc actctcccaa
     7621 cccaaaccaa ccggcgaagt ccagcgaatc gcgcgccacc acttcttggt agcgcccaca
     7681 agcgtgcgga aactgcccgc tggccaaccc gacgagacct gccgcaagca atcgctcgcg
     7741 agcgttgagc gtgtcgcccg cagcggcggc gcgttcggcc gctcgctgcc acagcgtggc
     7801 atccgcaccg gtccactcca gcgtttgcgc cagccagagg ttcgcctggg gaaagaacgg
     7861 gtcccgggcc agcgcagtcc gaaacgcagt aaccgcactg tctaggttcc aatccttgat
     7921 cgcggcgtgc ccgtcgaggt acgcctgcaa cgcgctgacc agcgtcgtcc ccatgaatcc
     7981 ggacttcgct gccggagggg cccgattcac gaggagttcc tctgcgagtt cctggatctg
     8041 ctgaggcagt ccggccccgt tcgccgccac ccgcctcagc gcacgcgcca ccgcgttacc
     8101 gtcggcgacg tcgtacagac ccgccacgat ctcaatcgaa tcgccaacgg ctccgtactc
     8161 gccccacacg agccggccgg cacgatgcgc gcgcgcatag gtaagtgagg cctgtagcga
     8221 gggattgcgg tcgccagcgc gacgcaacac gtccgctagc tcgagatctc cgactatgtg
     8281 gatgtccgtc caacgggaga acgcatcgct cagtagccgc tcgatcccgc cgccgtccag
     8341 cagaccagtc ggcgtcgaat cccgcggcgt gaacggcacc accaggacga cgctctggtc
     8401 gagaccggct cctagaattc gcggaatcac cacgaacccc gccaccccca cgcagaccgc
     8461 tcccgccacc cccgcccccc agcgccgccg ccgccgggcc agcacgctgc gcggcgtcca
     8521 gatgccgctc ggcgccgcca gcgcctccgc cgcctccgct cccgtcgcga accggtcctt
     8581 cgccttcggc tccagcatcc gcgccacgat ccccgccacc tcgggcggca ccgacggacg
     8641 cagcatcgtc aactccgcca gtcggttgcg cgctcgcaac gggcccgccg ccggcccctc
     8701 gggcggcgtc aacgccgccg gtggcagccc cgccagcatc tcgaacagca cgcaccccag
     8761 actgtagatg tcgctccgcc cgtcgagtgg ccccttcccc tccccctgct ccggactcat
     8821 gtagtcgggc gtccccaccg tcgtccccac cgccgtcacc cgcgcccccg ccgcgctgat
     8881 cgcccgcgcg atcccgaagt cgctcaccac cgcgtgctct tcctgcagca ggatgttctc
     8941 gggcttgatg tcgcggtgca ccacgccggc gcggtgcgca tagtccaggg catccgcgac
     9001 ctcgcgcgcg atcgtcagcg cgtccaccag cggcaactgc ccctcgcgct ccagccggtc
     9061 gcgcagcgac tcgccgcgca cgaacggcat gacgtagtag agcaggccgt ccgcgtcacc
     9121 cgagtcgtgc agcggcagga tgtggggatg ggcgaggttg gccgagaagc ggatttcctg
     9181 caggaagcgc tcggggccga gcgtgaccgc cagctcgggg cgcagcacct tcagcgcgac
     9241 gtcgcgctca ttgcgcagat cgtaggcgag gaagacgatc gccatccccc cgcggccgat
     9301 ctcccggtcg atggtgtaac gcccggcgag ggcggcctcc agacggttgc gcaggtcggc
     9361 catggccttg cgtcgctcga cttgtcggcc tcgaagtgcc cgagtttcag ggtaccgctc
     9421 cacgccaacc tccgccatcg gcggcagacc tacactggcc gcgtatgccc ttccaatctc
     9481 cgctatgagg ggtcaagcgg ccgtcaattg gccaacccct tggctagagc cactttgccc
     9541 gctagcttgt ggggctccgg atggcagact acacgtttgc gcgcggacag ctgctgctcc
     9601 tcgccgggac ggccaatcgg ccgctcgccg aggagatggc gcagcatttg aatcaaccgc
     9661 tgtgcgcggt caccatccgg catttcgccg acggcgagtt gttcgtgaag atcgacgaga
     9721 acgtgcgcgg gcgggacgtc ttcatcatcc agcccaccaa tccgccggcg gagaactatc
     9781 tggagttgct gctcctgatc gacgccgccc gccgggcgag cgccgcgcgg atcacggccg
     9841 tgctccccta cttcgggtac gcgcgccagg accgcaagga ccagccgcgg gtggcggtga
     9901 gcgcgaagct gatggcgaac ctgatcactc acgcgggcgc cgatcgcgtg ctgggcatgg
     9961 atttccattc gcaccagctg caggggttct gggatatccc ggtggaccac ctgtacgcgg
    10021 cgccggtgtt cgtctcgcac ttccggacga aggggctgcg ggagccggtg gtggtggcgc
    10081 ccgacgtggg atcggccaag atggcccgcg gctatgccaa gcggctgaac gcgtcgctcg
    10141 cgatcatcga caagcggcgg ccgtcggcga acgtcgcgga agtggtgaac gtcgtcggag
    10201 aggtcgaggg gatggactgc ctcatccccg atgacatgat cgatacggcg gggaccatca
    10261 ccgaagccgc cgcggcattg aagcggctgg gcgcccagcg catcttcgcg ttcgccacac
    10321 acgcgctgct gtcggggccg gccgtggagc ggttgcggag ctcgccgatc gaggaagtga
    10381 ccgtgaccaa caccatccgg ctccccgagg accggaagtt cgaaaaactg aagatcctgt
    10441 cgatcgccgg cctgatggcc aaggcaatcg gctacgagca ctcgaaccag tcggtgagct
    10501 cgctgttcga ctgaaacccg tggaccgcag actgaggacg gacgaggtat ggcacaattc
    10561 gtgacgctcg cgacgagccc gcgggacaag accggcaagg gcgcggcgcg gcagatccgc
    10621 ttccgcaaga aggtcccggc ggtgatttac ggacacggcc ggcccagcca gcccctcgag
    10681 ctcgacgcca aggcgctgga gctggcactc atcggcatcg agcccgcgag caccatcatc
    10741 gagatcgagc tcgagggcaa gaagtcgcgc acactgattc gcgagatcca gcggcacccg
    10801 gtgcgtcccg acatcatcca tgtggacttc tacgaagtcc gcgcgggcga gaaggtgacg
    10861 ctccagatcc cgatccgcct caccggcacg gcggacggcg tccgcaacgc cggcggcgtg
    10921 ctcgaccagg tgatgcgtga ggtggagatc gaggtgctcc ccgagcacat ccccgaccgg
    10981 gtggagctcg acgtgacgcc gctcctcatc ggccactccc tgcacgtccg cgatctcgtc
    11041 atccccaacg cgacgatcct gacgcaggcg gatctcacga tcgccaccgt ggtgccgccg
    11101 cgcgccgagg aggtcgccgc gccgacgccc gaggccgcgg ccgaggtcgc cgagcccgag
    11161 ctgatccgca aggtgcggga ggaagagggt gaggagggtg cggagggcga agccgccggt
    11221 gaagccaagg ggaaggcggc ggctccagaa gcgaaagccg agaagcccaa gggcggtaaa
    11281 gagggctgag cggcttgcgc gccgtcgtcg cgctgggcaa tcccggcccg gagtacgcgg
    11341 cgacgcgcca caacgtgggc ttctgggtgg cggacgcgct cgtcgcgcac tgggggtttc
    11401 ccccctttcg gcgcggcgag cgcgctcgcg tttccgaggg gcaggtggac gggaccaccg
    11461 ttcgcctgct caagcccacg accatgatga accgcagcgg cgcggccctc gccggcctgc
    11521 gcgccgaccc cggcttcgtg cccgccaccg atctgctcgt cgtcgtggac gacttcaata
    11581 tcccgctggg cacccatcgg ctgcgggcct ccggctcggc cgggggccac aacgggctca
    11641 agagcatcga aggcgccctg cagtcgcagg actacgcgcg gctgcgcatc ggcgtagggc
    11701 ccgtcccgcc gggagagccg tgggaagatt tcgtgctgtc acccttcgcc gacgaggagc
    11761 gcgagcaaat ggaggcgctg ctgccgcagc tggtcgccgc ggtggctggg tgggtccgtc
    11821 aaggcgtggt cagctgacct gccaggttct ccctttcctc agtagctccg ccagctcgcc
    11881 ccgcgacccc gccgccgcca tctgggcttc gacttcccgc tcgtaggacg tcgtgggatc
    11941 gcagtacaac acgcccaacg ccatcgggaa cgccggcggc tgcagtgcgg cgagcagcgt
    12001 ggcgagcgtg cggttcgttt cgtcgtgcac caggagatcc gtttcggaga tgccgccctc
    12061 gcccggcgtc accacctcca gctcgagctt ccccg
//
LOCUS       DBNGZW010000460         2536 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1474543, whole genome shotgun sequence.
ACCESSION   DBNGZW010000460 DBNGZW010000000
VERSION     DBNGZW010000460.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1474543"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="AC16JC_10860"
     CDS             <1..357
                     /locus_tag="AC16JC_10860"
                     /inference="COORDINATES: protein motif:HMM:NF022588.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HIR5165237.1"
                     /translation="NFATRFTSSADLVVAGQTVPAGTYTLWTLPSPTGWKLIFNKQTK
                     APCATAAECADPRRANLWGTDYSADSDFVRVDMQTAPLATPLEQFVITLEPQGDGGVL
                     AIAWEKTRAWVNLAKK"
     gene            496..924
                     /locus_tag="AC16JC_10865"
     CDS             496..924
                     /locus_tag="AC16JC_10865"
                     /inference="COORDINATES: protein motif:HMM:NF046506.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6941 family protein"
                     /protein_id="HIR5165238.1"
                     /translation="MHLDFAVVADYALVDQTGKVSVLVIFQHIWVQQFPAMHPRLHLV
                     LRLKGKRTEIGEHRVQIRLADESGTEILGGSGSITFAEPPAGVTDIEAPAILAFDVPF
                     THPGMYRFEISIDGERAAEVPLSVSQMPQPGAAPDAPGIH"
     gene            905..1936
                     /locus_tag="AC16JC_10870"
     CDS             905..1936
                     /locus_tag="AC16JC_10870"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HIR5165239.1"
                     /translation="MRRGSISLNRTTTPWLLLLTAAAVALGWDLGGHRFLDPDEGRNA
                     EVAREMAETNDYVVPHLDGLPYLDKPVVYFAAAALGMEVLGPREAAARLPALLATFAT
                     IAVVAWFARRRWGSAAGWLAGVALATMPMAAAYARTTIFDSTLSFFTTSAIICFTVER
                     PVLAWAAIGAGGITKGPVAILIPLATLIPWSLLAGVPVRRLFPWRGLLAFAVVALPWF
                     FAVTARIPEFPHYVFVRETFERVTTRSFHRTAPFWYFLPILPLAAFPWIVPALSRLTA
                     ARIRWSWLARKVNENARDVWLLAAWVLGPLVFFSLNQSKLPQYVLPLMPAFALAAART
                     LSREGAAAG"
     gene            1958..>2536
                     /locus_tag="AC16JC_10875"
     CDS             1958..>2536
                     /locus_tag="AC16JC_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165240.1"
                     /translation="MPLGLALATVTLWLPGSIPVTLPEQAAIPFTARALAAALLASAA
                     LVTLAARRGSVPLAAAGYALVTLALPFASGPLLRAVGDDRSAHTLATVIPDRASVVAV
                     AAYPPSLPFYLRRPILIATSHARELTSNYIADYADHYRTFPGSPLLPAEAWRERLARC
                     GDPTVFVTRAGDRGARAALAQLPLLFADGHYAA"
BASE COUNT          335 a          990 c          793 g          418 t
ORIGIN      
        1 aacttcgcga cgcggttcac cagctcggcc gacctcgtcg tggccggcca gacggttccc
       61 gccgggacgt atacgctctg gaccctgccc tcgcccaccg gctggaagct gatcttcaac
      121 aagcagacca aggcaccctg cgccaccgcg gccgagtgcg ccgaccccag gcgggccaac
      181 ctctggggca ccgactacag cgccgactcc gacttcgtcc gcgtggacat gcagaccgcc
      241 ccgctggcga cgccgctgga gcaattcgtc atcaccctgg agccgcaggg cgacgggggg
      301 gtgctcgcca tcgcctggga gaagacccgc gcgtgggtga acctcgccaa gaagtgagtg
      361 tggccgcgta gtgcgtggtg cgtggtgcgt ggtattatcc gggggcaatc ttggccgcgg
      421 ctccgacggc ggcaagcgta accggatgac ggccttggac cgtcgagtac cacacaccac
      481 ggaccacgca cctccatgca cctggatttc gccgtcgtcg cggattacgc cctcgtcgat
      541 cagaccggga aggtctccgt gctggtcatc tttcagcaca tctgggtgca gcaatttcct
      601 gcaatgcacc cgcggctgca cctcgtcctg cgcctgaaag gaaagcgcac cgagatcggg
      661 gagcaccggg tgcagatccg cctcgccgac gaatcgggca ccgagatcct gggcggcagc
      721 ggcagcatca ccttcgccga gccccccgcc ggcgtgaccg acatcgaagc cccggcgatc
      781 ctcgccttcg acgtgccctt cacgcacccc gggatgtatc ggttcgagat cagcatcgac
      841 ggcgagcgcg ccgcggaggt cccgctcagc gtcagccaga tgccgcagcc cggcgcggcg
      901 cccgatgcgc cggggatcca ttagcctgaa tcgcaccacc accccctggc tgctgctcct
      961 caccgcggcc gcagtggcgc tcggctggga cctgggtgga caccgcttcc tcgatcccga
     1021 tgaaggccgc aacgccgagg tcgcccgcga gatggcggag accaacgact acgtcgtccc
     1081 ccatctcgac gggctcccct acctcgacaa gccggtggtc tatttcgcgg ccgccgcact
     1141 cggcatggaa gtcctcggtc cccgggaggc ggcggcgcgc ctccccgcac tcctggccac
     1201 cttcgcgacc atcgccgtcg tcgcgtggtt cgcccgccgc cgctggggaa gcgcggcggg
     1261 gtggctggcg ggagtggccc tggccaccat gcccatggcg gcggcctacg cccgcaccac
     1321 gatcttcgac agtacccttt ccttcttcac cacctcggcc atcatctgtt tcaccgtgga
     1381 gcgcccggtc cttgcctggg cggcgatcgg cgccggcgga atcaccaagg gacccgtcgc
     1441 gatcctcatc cccctcgcca cgctgatccc ctggtccctg ctcgccggcg tgccggtgcg
     1501 gcgcctgttc ccgtggcgcg ggctgctcgc attcgccgtg gtcgccctgc cctggttctt
     1561 cgccgtgacg gcgcgcatcc cggagttccc tcattacgtc ttcgtgcgcg agaccttcga
     1621 gcgagtgacg acgcggagct ttcaccggac cgcgccgttc tggtatttcc tcccgatcct
     1681 gccgctcgcg gcgttcccct ggatcgtgcc ggcgctgtcc cgactcaccg ccgcacggat
     1741 ccgctggagc tggctcgccc gcaaagtgaa cgagaacgcc cgcgacgtgt ggctgctcgc
     1801 cgcctgggtg ctgggacccc tggtgttctt ttcgctgaac cagtccaaac tgccgcagta
     1861 cgtgctgccc ctcatgccag cattcgccct ggcggcggcg cgcacgctct cccgcgaggg
     1921 agccgccgcc ggctgaaagg cctacgtcat cgccgccgtg ccgctcggcc tggcgctggc
     1981 gaccgtcaca ctgtggctgc caggctcaat ccccgtgacc ctgcccgagc aggcggcgat
     2041 ccccttcacc gcccgagcgc tcgccgctgc gctgctcgct tccgcggcgc tcgtgaccct
     2101 cgcggcccgg cgcggcagcg tgccgctcgc cgccgccggc tacgctctcg tgacgctcgc
     2161 gctccccttc gcctcgggtc ccctgctgcg tgccgtgggc gacgatcgct cggcccacac
     2221 gctggcgacg gtgatccccg accgggcgtc ggtcgtcgcc gttgccgcgt atcctccgtc
     2281 gctcccgttc tacctgcgcc gacccatcct catcgccacg tcgcacgccc gtgagctcac
     2341 gagcaactac atcgcggact acgcggatca ctatcgcaca ttccccgggt cgcccctgct
     2401 cccggccgag gcctggcgcg agcgcctcgc ccgctgcggc gatcccacgg tgttcgtgac
     2461 ccgcgccggc gatcgaggcg cccgggccgc actggcgcag ctgcctcttc tcttcgccga
     2521 cggccactat gccgcc
//
LOCUS       DBNGZW010000461         3039 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_315520, whole genome shotgun sequence.
ACCESSION   DBNGZW010000461 DBNGZW010000000
VERSION     DBNGZW010000461.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_315520"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1335
                     /locus_tag="AC16JC_10880"
     CDS             <1..1335
                     /locus_tag="AC16JC_10880"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017463570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HIR5165241.1"
                     /translation="FPEGFLWGAATSAYQIEGSPLADGAGASIWHRFNHTPGKIPNGE
                     TGDVACDHYHRAAGDVELMRALGLQAYRFSIAWARVQPTGRGAVNGAGLAFYDRLVDL
                     LLRQGIRPVVTLYHWDLPAALDDQGGWLNRDVADWFAEYARVVVRALGDRVALWTTLN
                     EPWVVSDAGYLHGTHAPGHADPAGAPVASHNLLRAHGAGLQAIRAEGGKQAGLVVNLE
                     PKVPASDRAEDVAATRRADAYMNRQFLDPVFLGRYPEELAQVFGSAWPTFPETDFVLI
                     RQPLDFLGVNYYTRGVIRDDPSAPPVRVAHDRQSGHVYTMTNWEVYPPAFTDVLVWVR
                     DRYGPVPLYVTENGAAFEDPPRAGGALVDDPQRVMYLRDHLAALHGAITQGVDVRGYF
                     VWSLLDNFEWSAGYGPRFGLYHVDFETQQRTPKASARFYSEVIRTHGAVLAT"
     gene            complement(1344..>3039)
                     /locus_tag="AC16JC_10885"
     CDS             complement(1344..>3039)
                     /locus_tag="AC16JC_10885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007191557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GH36-type glycosyl hydrolase domain-containing
                     protein"
                     /protein_id="HIR5165242.1"
                     /translation="AECCVLLGEGDSRAAVLELCRRYAGATAVSEALNDVRRFWRDTL
                     GRLQITTPAPAIDLVVNGWLAYQNLSCRMWGRSAFYQSGGAYGFRDQLQDSSALVWLS
                     PETTRSQILLHAAHQFPEGDVLHWWHPPDSRGIRTRFADDPLWLPFVTSEYIRHTGDG
                     GVLREAAGFLEARTLAAGEDYAFLFPRRSGTDADLYEHCCRAVDRSLTRGAHGLPLIA
                     GGDWNDGMNRVGRLGRGESVWLGFFLYEVLQRVLPWCERRRDRTRIERYRAALPSLAA
                     ALNAGGWDGAWYRRAYYDDGVPIGSAQSDECRIDAIAQAWAVLSGAAPPDRAALALDA
                     LEEHLVDARDGLIRLLTPPFDRTAHDPGYIKGYVPGVRENGGQYTHGVLWAVRALAEL
                     GREERAAALLEMLSPVTRAHGPEAVAVYQAEPYVVAADVYGAAPHVGRAGWTWYTGSA
                     GWMYRVAVESVLGLTMKEGASLRLRPCIPASWPGFSLRYRVPGHETQYAIAVTQERPC
                     TARTVASLDGTPLEVAAGAVTIPLLTDGVEHDVHVRLGKDVGRRYYARPAAGDRAD"
BASE COUNT          470 a         1084 c         1028 g          457 t
ORIGIN      
        1 tttcccgagg ggtttctctg gggcgcggca acttccgcct accagatcga gggctcgcca
       61 ctcgccgacg gcgccggcgc gagcatctgg caccgcttca accatacgcc cggaaagatc
      121 cccaacgggg agaccgggga cgtcgcctgc gatcattacc atcgcgccgc gggggatgtg
      181 gaactgatgc gcgcgctcgg actgcaggcg tatcgcttca gtatcgcgtg ggcgcgggtc
      241 cagccgacgg gacgcggtgc ggtgaatggt gcggggctcg ccttctacga tcgcctcgtg
      301 gacctgctcc tgaggcaggg catccggccc gtggtcaccc tgtaccactg ggacctcccg
      361 gccgcactgg acgatcaggg gggctggctc aatcgcgacg tggcggactg gttcgccgaa
      421 tacgcgcggg tggtcgtgcg tgcgctgggc gaccgggtcg cgctgtggac gacgctcaac
      481 gaaccctggg tggtgagcga tgccggctac ctgcacggca cgcacgcgcc gggtcacgcc
      541 gatccggccg gggcgcccgt cgcgagtcac aacctgctgc gcgcacacgg ggccggcctc
      601 caggcgatcc gggccgaagg cggcaagcag gcgggcctcg tcgtgaatct cgagcccaag
      661 gtccccgcgt ccgaccgtgc ggaagacgtc gccgcgacgc gccgcgccga cgcctacatg
      721 aaccgccagt ttctcgaccc ggtctttctc ggcaggtatc ccgaggagct ggcccaggtg
      781 ttcgggtccg cctggccgac gttccccgaa acggatttcg ttctcatccg ccagccgctc
      841 gattttctgg gcgtcaacta ctacacccgc ggcgtcatac gcgacgatcc gtccgcgccg
      901 ccggtccgcg tcgcgcacga ccggcagtcc ggccacgtct atacgatgac gaactgggaa
      961 gtgtatcccc ccgccttcac cgacgtgctc gtgtgggtgc gggatcgcta cggacctgtc
     1021 ccgctctacg tgacggagaa cggggctgca ttcgaagacc cgccgcgggc cggcggagca
     1081 ctggtggatg atccccaacg tgtaatgtat ctgcgggacc atctcgcggc gctgcatggc
     1141 gcgatcactc agggcgtcga cgtgcgggga tacttcgtct ggtcgttgct cgacaacttc
     1201 gagtggagcg cgggctacgg cccgcgcttc ggactctacc atgtggactt cgagacgcag
     1261 cagcgcacgc cgaaggcgag cgcccgcttc tacagcgagg tgatccgcac ccacggcgcc
     1321 gtgctggcaa cctgacatgg tgctcaatct gcccggtcac ctgccgccgg gcgagcgtag
     1381 tagcggcggc cgacgtcctt cccgaggcgt acgtgcacgt cgtgttccac gccgtccgtc
     1441 agcagcggta tcgtgacggc accggcggcg acctcgagcg gcgtgccgtc caggctggcg
     1501 acggtccgcg ccgtacacgg ccgctcctgc gtcacggcga tcgcgtactg cgtctcgtgc
     1561 ccgggaaccc ggtagcgcag cgagaagccg ggccatgacg ccgggataca gggcctgagc
     1621 cgcaggctcg cgccttcctt catcgtgaga cccagtaccg actccaccgc gacccggtac
     1681 atccacccgg ccgatccggt ataccaggtc cacccggccc ggcccacgtg cggtgcagcc
     1741 ccatacacgt cggcggccac cacgtacggc tcggcctggt acaccgccac cgcctcgggc
     1801 ccgtgtgcgc gagtcaccgg actcagcatc tcgagcagtg ccgcagcccg ctcctcacga
     1861 cccagctccg cgagggcgcg gacggcccac aacacgccgt gggtgtactg gccgccgttc
     1921 tcccgcacgc ccggcacgta gcccttgatg tagcccggat cgtgcgcggt ccggtcgaac
     1981 ggcggcgtga gcagccggat gaggccgtcc cgtgcgtcca ccagatgctc ttccaatgcg
     2041 tcgagcgcga gcgcggcgcg gtcgggcggg gctgcgccgg agaggacggc ccacgcctgg
     2101 gcgatcgcat cgatgcggca ttcgtcgctc tgcgcggagc cgatgggcac gccgtcgtcg
     2161 taatacgcac ggcgatacca ggcgccgtcc cagccgcccg cgttcagggc cgctgcgagg
     2221 gacggcaggg cggcgcgata gcgttcaatc ctcgtcctgt cgcggcggcg ctcgcaccat
     2281 ggcaataccc gttgcaggac ctcgtagaga aagaagccga gccacacact ctcgccgcgt
     2341 cccaggcggc ccacccggtt cataccgtcg ttccagtcgc cgccggcgat cagcggcagg
     2401 ccgtgcgcgc cccgggtcag cgaccggtcc acggcacggc agcagtgctc gtacagatcg
     2461 gcgtccgtgc cgctccggcg ggggaacagg aacgcgtagt cttcgcccgc ggcgagggtc
     2521 cgggcttcga gaaatcccgc cgcttcccgg agcacgcccc cgtcccccgt gtggcggatg
     2581 tactcgctcg tcacgaacgg cagccagagc gggtcgtcgg cgaaacgggt tcggatgccg
     2641 cggctgtccg gcggatgcca ccagtggagc acgtcaccct ccggaaactg gtgggcggcg
     2701 tgcagcagga tctgtgagcg tgtggtttcc ggcgaaagcc acaccagcgc ggacgaatcc
     2761 tgcagctggt cgcggaagcc gtacgcgcca ccggactgat agaaggccga ccggccccac
     2821 atgcgacagc tcaggttctg gtaggcgagc cacccattca ccacgaggtc gatcgccgga
     2881 gcaggtgtcg tgatctggag ccgaccgagg gtatcgcgcc agaagcggcg cacgtcattc
     2941 aacgcttcgg atacggccgt cgcgcccgca tagcgccggc acaactcgag aaccgccgcc
     3001 cggctgtcgc cttcgcccag caacacgcag cactcggcc
//
LOCUS       DBNGZW010000462         2317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1612097, whole genome shotgun sequence.
ACCESSION   DBNGZW010000462 DBNGZW010000000
VERSION     DBNGZW010000462.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1612097"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1228
                     /locus_tag="AC16JC_10890"
     CDS             <1..1228
                     /locus_tag="AC16JC_10890"
                     /inference="COORDINATES: protein motif:HMM:NF019912.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR5165243.1"
                     /translation="NGAFVAELPGVYTVWASIGAHTAEAVVRVEARRVTRGIEVRAHL
                     PIAQRAAEVWVHPNGQCAYLSTIADRVYAIDIADPGHPRIVDSMMNDARINNDVMTTE
                     DGRYGVFSREGASNRKNGIMIFEASDPCHPKVIAEYTETVSGGVHSSYVYRGHVYLTD
                     DATGSMRVLDIQDPYHPTQVARWQTEQTEAGRYLHDIMVVDGLAYLAYWNDGLVILDV
                     GNGMKGGSPEHPQLVSQFKYDLNATYARVEQLWGPGFVRGTHTAWRAGRYVFVGDEVY
                     AARPYTGLKGGNDLTFGRLHVIDVSDIEHPQEVAWYEPTDGGVHNVWVVGDTLYLGNY
                     QGGARVLDVSGELKGDLLRQGREMSWILTADSTGAQPHTPFAWGAVVRDGNIYVPDIN
                     SGLWILRLEPKREVTP"
     gene            complement(1246..1782)
                     /locus_tag="AC16JC_10895"
     CDS             complement(1246..1782)
                     /locus_tag="AC16JC_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165244.1"
                     /translation="MPRWVTPLLVGGVILALPLSGQDPTPPAESVPAASPPAPPAPVL
                     PSPGQERYLQGLRTAGRGVAQIKDGMNRLSRVQAGRDTTQIRQAARRLGGLCSAARGF
                     LTSGRGRMEPSVYEAPVRRPARDLVLQVDSLSLMAKECQLTAGKSPAPVAAALLTRIR
                     TYEAALAAFRTAIGLPNR"
     gene            1844..>2317
                     /locus_tag="AC16JC_10900"
     CDS             1844..>2317
                     /locus_tag="AC16JC_10900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165245.1"
                     /translation="MTVPATTRAWRMFEDRAEALSHFFARAGEAPRFIAFDDTVGCPL
                     DQALAVVEWTEAAGILHDDDIIHAAWTRPDAAAAVVERRDGERRSFAYFGPRMDAPYA
                     DPGECELIYDGPGVRAYAFGERGPALAHFLRATQGTGAILSLLARRGPELRHVRRW"
BASE COUNT          379 a          786 c          796 g          356 t
ORIGIN      
        1 caacggcgcg ttcgtcgcgg agctccccgg ggtctatacc gtgtgggcct cgatcggtgc
       61 gcacacggcg gaggccgtcg tgcgggtgga ggcgcggcgc gtcacccgcg gcatcgaggt
      121 ccgtgcccat ctgcccatcg cccagcgagc cgccgaagtc tgggtgcacc ccaacggaca
      181 gtgcgcctac ctctcgacca tcgccgatcg tgtctacgcc atcgacatcg ccgaccccgg
      241 gcatccccgg atcgtcgatt ccatgatgaa cgacgcacgc atcaacaacg acgtgatgac
      301 gaccgaggac ggcagatacg gcgtcttctc acgcgaaggc gcctcgaacc gcaagaacgg
      361 catcatgatc ttcgaggcga gcgatccgtg tcacccgaag gtcatcgccg aatacaccga
      421 aacggtcagc ggcggggtcc acagctccta cgtctaccgg gggcacgtgt acctgaccga
      481 cgacgcgacc ggctcgatgc gcgtgctcga catccaggat ccctaccacc ccacccaggt
      541 ggcgcggtgg cagaccgagc agacggaagc cggccgctac ctgcacgaca tcatggtcgt
      601 ggacggcttg gcctacctcg cgtactggaa cgacgggctc gtcatcctgg acgtgggtaa
      661 cgggatgaag ggcggcagtc cggagcatcc acagctcgtc tcccagttca agtacgacct
      721 caacgcgacg tacgcccgcg tcgagcagct gtggggtccg gggttcgtgc ggggcacgca
      781 caccgcgtgg cgcgccggcc gctacgtgtt cgtgggcgac gaggtgtacg ccgcccgacc
      841 ttacaccggg ctcaagggcg gcaacgatct cacgttcggc cggctgcacg tcatcgacgt
      901 atccgacatc gagcaccccc aggaggtggc ctggtacgaa cccaccgacg gcggtgtgca
      961 caacgtgtgg gtggtggggg acacgctcta cctcggcaac taccagggtg gcgccagggt
     1021 gctcgacgtc tccggtgagc tcaagggcga cctgctgcgg caggggcggg agatgagctg
     1081 gatactcacc gccgattcga ccggcgcgca gccccacacg ccgttcgcct ggggcgccgt
     1141 cgtgcgcgac ggaaacatct acgtgccgga tatcaactcg ggactgtgga ttctccggct
     1201 ggagccgaag cgggaggtga ccccctaacc gcgaacgcga cgccgctacc tgttcgggag
     1261 tccgatcgcc gtccggaagg cggccagcgc cgcctcgtac gtccggatgc gggtgagcag
     1321 agcggcggcg accggcgccg gtgacttgcc ggccgtcagc tggcactcct tggccatgag
     1381 cgagagcgaa tcgacctgga ggaccaggtc ccgggcgggc cgacgcaccg gcgcctcata
     1441 aacactcggc tccatcctgc cgcgtccgct cgtcaggaag ccgcgcgcgg ccgagcatag
     1501 gccgcccagc cgccgtgccg cctggcggat ctgcgtcgta tcgcgtcctg cctgcacgcg
     1561 gctcagacga ttcatcccat ccttgatctg ggccacgcct cgaccggcgg tgcgcaggcc
     1621 ctggaggtag cgttcctgcc ccggactggg gaggacggga gccggggggg cgggaggact
     1681 cgccgcggga accgactccg caggaggggt cgggtcctgt cctgacaggg gcagcgcgag
     1741 gatcactccg ccgacgagga gaggtgtaac ccaacgaggc atgcgagctt cctgcattct
     1801 ggactccggc cactttgcct aggccatggg ttataatctg gtcatgaccg ttcccgcaac
     1861 cactcgggca tggcgcatgt tcgaggatcg cgccgaggcg ttgagccact tcttcgcccg
     1921 ggcgggtgaa gctccccggt tcatcgcctt cgatgatacg gtgggatgcc ctctcgatca
     1981 ggccctggcc gtcgtggaat ggaccgaggc cgccggcatc ctgcatgatg acgacatcat
     2041 acacgccgcc tggacccggc ccgatgccgc ggccgctgtg gtcgagcgcc gtgacgggga
     2101 gcgacggtcg ttcgcgtatt tcggaccgcg gatggacgcg ccctatgccg atcctgggga
     2161 gtgcgagctg atctacgacg gtccaggggt gcgggcctac gcattcggcg agcgtggacc
     2221 cgcactggcc catttcctcc gggcgaccca ggggacgggg gccatcctgt cgctgctcgc
     2281 ccggcgcgga cccgagctgc ggcacgtgcg gcgctgg
//
LOCUS       DBNGZW010000463         6085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1269372, whole genome shotgun sequence.
ACCESSION   DBNGZW010000463 DBNGZW010000000
VERSION     DBNGZW010000463.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6085
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1269372"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="AC16JC_10905"
     CDS             <1..242
                     /locus_tag="AC16JC_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR5165246.1"
                     /translation="DQLDRFLPLVATAENVAFNTYSASSTVRVAVHGWLASTHHLENI
                     EGKYDVTGVGIARAGDGAFYYTQLFVRRSASGGRP"
     gene            complement(223..1269)
                     /locus_tag="AC16JC_10910"
     CDS             complement(223..1269)
                     /locus_tag="AC16JC_10910"
                     /inference="COORDINATES: protein motif:HMM:NF019352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR5165247.1"
                     /translation="MSLYRTTGARRLARALALAATAGAPACGRTILSYRPRTRTYYVR
                     AEPVRWDYLPTGYDSVMGRPPPPPWGDSLVYRKLRYVGYTDSTFTTRAPEPPWIGILG
                     PVLRACTGDTIRVVFRNATGQPLSMHPHGVRYDERNEGALYNPPRGGGDSVSAGATFT
                     YNWLAGRESGPLPGEPSTRVWLYHSHVHPEAEIEAGLVGAIIVADPTRADPSTALPND
                     VDREFVTLWMIFNENGPASAPRDEEMNLKHTINGRFFGSLRGLVMNKGDRVRWYVTAL
                     GSESDLHTPHWHGAKLLLDGRSYVDVIEVLPGSMKLADMVARNPGVWMLHCHVNDHME
                     AGMYATYTITDALR"
     gene            complement(1284..1862)
                     /locus_tag="AC16JC_10915"
     CDS             complement(1284..1862)
                     /locus_tag="AC16JC_10915"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF013492.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR5165248.1"
                     /translation="MAVRRIRLLGDPMLRQRCQLVAKPTSGAARLVADDLRDTLKWAK
                     EKYGMGRALAAPQIGAPVRMIVMDVDGARQRTTLVNPEITDVGSEDFEVWDDCFSFPE
                     LLVRVNRAYRITVQFTDLKGKTHSLECEGPLAELLQHEIDHLDGILALDRAVGLDPFA
                     FRSEWEKHHDKSERFASPHPREAPEEAAIASS"
     gene            1984..2601
                     /locus_tag="AC16JC_10920"
     CDS             1984..2601
                     /locus_tag="AC16JC_10920"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HIR5165249.1"
                     /translation="MPLAAVVLAGGRSARMGTPKALLDFRGEPFVVRILEAVEALDLK
                     TRVVVVGPPPDGIAVRAAVTGRDCTVVENSDVDGGPIASLRRALDALDAIHPPAALVW
                     PVDLPHIHVATIERLVETYRRRHAPVTLPTFAGRRGHPVIWDQSLFAELRSSDAATRE
                     GARAVLHAHLDEAVLVPVDDPAVTDFINTPADYERLIREINRDAY"
     gene            2695..3327
                     /locus_tag="AC16JC_10925"
     CDS             2695..3327
                     /locus_tag="AC16JC_10925"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5165250.1"
                     /translation="MAAAAPGTRTHTPTRGSAHVRAKRQRRRVEILRAALRAFRDKGY
                     HATTLDDIARQLGVRKTALYHYFPDKEAILHACHREGLEEVAGIVAAARRRPSFAEQL
                     AHAIREHVRVMTDTLEGSSLAFEVSALSPRHQAEVIAARDRYEQALRDIIHRGVAAGE
                     FRAVDPKLAVFAILGAINWVARWYSPTGSVHPPTLGAHFADHLVRGLLAP"
     gene            3324..4406
                     /locus_tag="AC16JC_10930"
     CDS             3324..4406
                     /locus_tag="AC16JC_10930"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5165251.1"
                     /translation="MTTSTSIPTAPSTRQFGSRPAASFAFREVLYEKAGGIARITINR
                     PHNYNAYSTAALEELATAFRDASFDDAVGVIVLTGAGDRAFCTGGDVKEYADAYVGTP
                     RDYWKYMALFRAYIESILNTGKPVIARINGIAVGGGNESQLACDLSVIAQHAYLKQVG
                     THVGSVACGGATQWLPIAIGDRRAREMLLLNEPVPAARALEWGLVSRVAPSVRKGDAW
                     IESATAEQIAAAQKGRDGYRIDLTQLDAAVDEIAHRLLETFPECTRYTKQQTNFWKDL
                     AWHQTVRHAQDWLSLHYASWEPVEGMRAFTEKRPARYALLRERAAQGGSSESPWGAPV
                     RDCQACGATNLPASHTHCGLCGARLS"
     gene            4385..5104
                     /locus_tag="AC16JC_10935"
     CDS             4385..5104
                     /locus_tag="AC16JC_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012869844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5165252.1"
                     /translation="MRRTALVTGGARGIGRAIVAALADEAWVASLDCDFPEGPGAAAA
                     TFAGDVRDGAAVAAAVERITRDRGGLDWVVCAAGIVRDRVSWKMSDAEWDEVLEVNLT
                     GAFRSVRAALPALRRSVSGRVVFVSSINGLRGNFGQANYAAAKAGLIGLARSLAKELA
                     RDGVTVNVVAPGFIDTAMTRALPQEVRARAVGRTPLGRVGTPEEVGALVRFLCGDAAG
                     FITGAVVPVDGGQLLGGGLER"
     gene            5101..5859
                     /locus_tag="AC16JC_10940"
     CDS             5101..5859
                     /locus_tag="AC16JC_10940"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5165253.1"
                     /translation="MTVAATTEAGIGRIELNHPPLNILTRDVLRGIHAELRRLAEQPD
                     LRVLLLTAAGEHFSAGADVGEHLPPHHLSMIPDFLETVAALDAFPLPVIAAVRGKCLG
                     GGFELVQAVDLIVAGTGAGFGQPEIALGVFPPVACVLLPERVPPAVAARLIYHGGPMS
                     AAEAERWGLVTRLVPDEDLDRAALEEARSLARHSAVALRAAKHALRGSGAPARHAALR
                     AAGHIYLHELMDTHDAVEGLRAFTEKRRPVWRHA"
     gene            5856..>6085
                     /locus_tag="AC16JC_10945"
     CDS             5856..>6085
                     /locus_tag="AC16JC_10945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165254.1"
                     /translation="MTVLFPEWRDKPLDEILFQCRELVEDTDFPTVRLWREGGGKVVG
                     HFQVYFPDEIAHAAGLLPFKVRGAPVEPAQADS"
BASE COUNT          873 a         2118 c         2152 g          942 t
ORIGIN      
        1 tcgatcagct ggaccggttc ctgccgctcg tggcgacggc cgagaacgtc gcattcaata
       61 cctacagcgc gagcagtacg gtgcgcgtcg cggtgcacgg ctggctcgcg agtacgcacc
      121 atctcgagaa catcgaaggg aagtacgacg tcacgggcgt ggggatcgcc cgggccggtg
      181 acggcgcgtt ctactacacg cagttgttcg tgcgacggtc ggctagcgga gggcgtccgt
      241 gatcgtgtag gtcgcgtaca tcccggcttc catgtggtcg ttgacgtggc aatgcagcat
      301 ccacaccccc gggttgcggg cgaccatgtc cgccagcttc atgctgccgg ggagcacctc
      361 gatcacgtcg acgtagctcc ggccgtccag cagcagcttc gccccgtgcc aatgcggcgt
      421 gtgcaagtcc gactcggagc cgagcgccgt gacgtaccag cgcacgcggt ctcccttgtt
      481 catgacgagg ccgcggaggc tcccgaagaa gcgaccgttg atggtgtgct tcagattcat
      541 ctcctcgtca cgcggcgccg aagccgggcc gttctcgttg aagatcatcc agagcgtgac
      601 gaactcgcgg tccacgtcgt tcggcagcgc ggtggaggga tccgcgcggg tgggatcggc
      661 gacgatgatc gccccgacca ggccggcttc gatttccgct tccgggtgga cgtgcgagtg
      721 gtacagccac actctcgtgc tcggttcgcc cgggagcgga ccgctctcgc gtcccgccag
      781 ccagttgtag gtgaaggtcg cgccggcgct gacgctgtcg ccgccgccgc gcggcggatt
      841 gtacagggcg ccttcgttgc gctcgtcgta gcgcacgccg tgcgggtgca tcgagagcgg
      901 ttggccggtg gcgttgcgaa acaccacgcg gatcgtgtcg ccggtgcagg cgcgcagcac
      961 ggggccgagg atgccgatcc agggcggctc cggcgcccgg gtcgtgaacg tcgaatcggt
     1021 gtagccgacg tagcgcagct tccggtacac gagcgaatcg ccccagggag gcggcggcgg
     1081 ccggcccatg acggaatcgt agcccgtggg caggtagtcc caccgcaccg gctcggcgcg
     1141 gacgtagtag gtccgcgtcc gagggcggta ggagagtatg gtgcgaccgc acgccggtgc
     1201 gccggccgtg gcggcgagcg ccagggcgcg cgcaaggcgg cgggcgccgg tcgtccggta
     1261 cagcgacatc ggatggtgtg aggtcagctg gaggcgatcg ccgcttcttc gggcgcttcg
     1321 cgcgggtgcg gactggcgaa gcgctcgctc ttgtcgtgat gcttctccca ctcactgcgg
     1381 aaggcgaacg ggtcgagacc gacggcccga tccagcgcca ggatcccgtc caggtggtcg
     1441 atctcgtgct gcagcagctc ggccagcggg ccctcgcact ccagggagtg cgtcttcccc
     1501 ttgaggtccg tgaactggac cgtgatgcga taggcccggt tcacccggac caggagctcc
     1561 gggaacgaaa agcagtcgtc ccacacctcg aagtcctcgg agcccacgtc ggtgatctcc
     1621 gggttgacga gggtcgtccg ctgtcgcgcg ccatccacgt ccatcacgat catccggacc
     1681 ggcgcgccga tctgcggcgc ggccagggcc cgacccatcc cgtacttctc cttggcccat
     1741 ttgagcgtgt cgcgcaggtc gtccgccacg agccgggcgg ccccggacgt gggtttggca
     1801 accagctgac agcgctggcg cagcatcggg tcgcccagca ggcgaatgcg tcgtacggcc
     1861 atggggggac ctaatactgc tgtttcgccg ccgcggcgca acagggcatc tgcaatccct
     1921 tgtgccacaa cctgatagga tcctgtgccc caggacgccg cgggcggttt atcgtaccct
     1981 gtcatgcccc tcgcggccgt agttctggcg ggcggccgtt ccgcccggat gggtacgccc
     2041 aaggcgttgc tcgacttccg gggggagccc ttcgtggtcc gcattctcga ggcagtcgaa
     2101 gccctcgacc tcaagacgcg cgtggtcgtc gtcggtcctc cccccgacgg gatcgcggtg
     2161 cgggctgccg tcacgggccg cgactgcacc gtcgtcgaga actccgatgt ggacggcggt
     2221 ccgatcgcct cgctgcgccg tgccctcgac gcgctggacg ccattcatcc gcccgccgct
     2281 ctcgtctggc cggtggatct gccccacatc cacgtggcga cgatcgagcg gctggtggag
     2341 acgtaccggc ggcggcatgc tcccgtcacc ctgcccacgt tcgccggacg ccggggccat
     2401 ccggtgatct gggatcagag cctgttcgcc gagctgcgct cgagcgacgc cgcgacacgc
     2461 gagggtgccc gggcggtgct tcacgcgcac ctcgacgagg ccgtcctcgt gccggtcgat
     2521 gacccggccg tgaccgactt catcaacacc ccggccgact acgagcgcct gattcgcgag
     2581 atcaaccgcg acgcctactg acggcgcggt ggcgccggtc ttcatcttga cttcacgata
     2641 aatttttgaa ttgatgttca attttcttgc cggaccacct cgtgaggcgc cggcatggcc
     2701 gctgcagcac ccggtacacg cacgcacacc ccgacccgcg gctccgccca cgtgcgcgcc
     2761 aagcgccagc ggcggcgggt cgagatcctg cgcgccgccc tgcgggcctt ccgcgacaag
     2821 ggctaccacg ccaccacgct cgacgacatc gcccggcagt tgggcgtccg caagaccgcc
     2881 ctgtaccact acttccccga caaggaagcg atcctccacg cctgtcatcg tgaggggctc
     2941 gaagaggtcg ccgggatcgt ggcggcggcg cggcgccgcc cctcgttcgc cgagcagctc
     3001 gcccacgcga tccgcgagca cgtgcgggtc atgaccgaca ccctcgaggg ctcctcgctc
     3061 gcgttcgagg tctccgcgct ctcgccgcgc caccaggcgg aggtcatcgc cgcacgggac
     3121 cgctacgagc aggcgctgcg cgacatcatc caccgcggcg tcgccgccgg cgagttccgc
     3181 gcggtggatc cgaagctcgc cgtctttgcg atcctgggcg ccatcaactg ggtcgcccgc
     3241 tggtactcgc ccaccggctc ggtgcaccca ccgacgctcg gcgctcactt cgccgaccat
     3301 ctcgttcgag gactcctggc cccatgacca cttcgacttc catcccgacc gccccgtcca
     3361 ctcggcagtt cggctcccgc ccggcggcga gcttcgcgtt ccgcgaggtg ctgtacgaga
     3421 aggccggcgg gatcgctcgc atcacgatca atcgtccgca caactacaac gcctattcca
     3481 cggcggccct ggaagagctg gccaccgcgt tccgcgatgc cagcttcgac gacgcggtgg
     3541 gggtgatcgt cctgaccggc gcgggcgatc gcgcgttctg cacgggcgga gacgtgaaag
     3601 agtacgcgga cgcctacgtc gggaccccgc gcgactactg gaagtacatg gcgttgttcc
     3661 gggcctacat cgagagcatc ctcaacaccg gcaagccggt catcgcgcgc atcaacggca
     3721 tcgccgtggg cgggggcaac gagagccagc tggcctgcga tctgtcggtg atcgcgcagc
     3781 acgcctacct gaagcaggtg ggtacccacg tcggctcggt ggcctgcggc ggcgccacgc
     3841 aatggctgcc gatcgccatc ggcgaccggc gcgcccgcga gatgctgctg ctcaacgagc
     3901 ccgtgcccgc cgcacgggca ctggagtggg gcctcgtgag ccgggtcgcg ccgtcggtgc
     3961 gcaagggcga cgcgtggatc gaatcggcga ccgccgagca gatcgccgcc gcgcagaagg
     4021 gccgcgacgg ctaccgcatc gatctgacgc agctcgacgc cgcggtggat gagatcgcac
     4081 accggctgct cgagaccttc ccggaatgca cacgctacac caagcagcaa accaacttct
     4141 ggaaggatct cgcctggcac cagacggtgc ggcacgcgca ggactggctg tcgctgcatt
     4201 acgcctcctg ggagccggtc gaagggatgc gcgccttcac cgagaagcgc cccgcgcggt
     4261 acgcgctgct gcgcgagcgg gcggcgcagg ggggctcctc cgagtcgccg tggggcgcgc
     4321 cggtgcgcga ctgccaggcg tgcggcgcca cgaatctccc cgcctcccac acccactgcg
     4381 gtctgtgcgg cgcacggctc tcgtaacggg cggcgcccgc ggcatcggcc gggcgatcgt
     4441 cgccgcgctc gcggacgagg cgtgggtggc gtcgctggac tgcgacttcc ccgaagggcc
     4501 gggggcggcg gcggcgacgt tcgccggcga cgtgcgcgac ggcgcggcgg tcgcagccgc
     4561 ggtcgagcgg atcacgcggg accggggcgg gctcgactgg gtagtgtgcg cggcgggcat
     4621 cgtccgggac cgcgtgagct ggaagatgag cgatgcggaa tgggacgaag tgctcgaagt
     4681 gaacctgacc ggtgcgttcc ggtcggtgcg ggcggcgctg ccggcgctgc gccggtccgt
     4741 gtccggccgc gtcgtattcg tctcctcgat caacggcctg cgcggcaact tcggccaggc
     4801 gaactacgcc gccgccaagg ccggtctgat cgggctggcg cgctccctgg cgaaggagct
     4861 cgcccgcgac ggggtgacgg tgaacgtcgt ggcacccgga ttcatcgata cggcgatgac
     4921 ccgcgccctg ccgcaggagg tccgcgcgcg cgcggtgggc cgcacgccgc tgggccgcgt
     4981 gggcacgccc gaagaggtcg gggccctcgt gcgattcctg tgcggcgatg cggcggggtt
     5041 catcaccggt gccgtcgtgc cggtcgatgg cggtcaattg ctgggaggag gtctggaacg
     5101 atgacggtcg cggccacgac agaagcggga atcgggcgga tcgagctgaa ccatccaccg
     5161 ctcaacatcc tgacacggga cgtgctccgg ggcattcacg ccgagctgcg ccggctggcg
     5221 gaacaacccg acctgcgggt gctgctgctc acggccgctg gcgagcactt ctccgcgggc
     5281 gcggacgtcg gcgagcacct gccgccgcat cacctcagca tgatcccgga tttcctcgag
     5341 accgtagccg cgctggacgc cttcccgctc ccggtgatcg cggccgtgcg cgggaagtgc
     5401 ctgggcggtg ggttcgagct ggtgcaggcg gtggatctga tcgtggcggg caccggggcg
     5461 ggcttcggtc agccggagat cgccctcggg gtcttccccc cggtcgcctg cgtgctgctc
     5521 cccgaacggg tgccgcccgc cgtcgccgcc cgcctcatct accacggggg cccgatgtcc
     5581 gccgcggagg cggaacggtg gggactcgtc acgcgcctcg tgccggacga ggacctcgac
     5641 cgggcggcgc tcgaggaagc gcgcagcctc gcccgccaca gtgcggtcgc gttgcgagcg
     5701 gcgaagcacg cgctgcgcgg cagcggtgca cccgcgcgcc atgcggcgct ccgggcggcg
     5761 ggtcacatct atctgcacga gctgatggac acgcacgatg cggtcgaagg gctgcgcgcg
     5821 ttcaccgaga agcgccggcc cgtctggagg cacgcatgac cgtgctgttc cccgagtggc
     5881 gcgacaagcc gctcgacgaa atcctgttcc agtgccggga gctggtggag gacaccgact
     5941 ttccgacggt gcgcctctgg cgcgaggggg gcgggaaggt cgtcgggcat ttccaggtct
     6001 acttccccga tgagatcgcc cacgccgcgg ggctgctgcc gttcaaggtg cgcggcgcgc
     6061 cggtcgaacc ggcgcaggcc gattc
//
LOCUS       DBNGZW010000464         2268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_929251, whole genome shotgun sequence.
ACCESSION   DBNGZW010000464 DBNGZW010000000
VERSION     DBNGZW010000464.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_929251"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="AC16JC_10950"
     CDS             <1..627
                     /locus_tag="AC16JC_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165255.1"
                     /translation="GGSGNTDLYVKAAPGTAAAILRDTVVEAEFARAQGGLLRLTAPP
                     GVYRLALDVDSEGVVGRVREPVRLPVYSWAYVGVSSLVLATGDSLSDREAALAAMPAD
                     LTYPAGRAVATYAELYGLSRDSDGRARYRVRYSFAPLRSLAARLTRGASPVVFEFDRE
                     SEWRGTMLERLVIEPGRVPPGRYRVTLSVTDLPTNVKSATVALDITIR"
     gene            682..1626
                     /locus_tag="AC16JC_10955"
     CDS             682..1626
                     /locus_tag="AC16JC_10955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HIR5165256.1"
                     /translation="MFVNLVPEFLACLAAPDPVAASHSYLERHGPVLASYWHNYVLDL
                     ESPHAERVIREAVRADRDDLRSLLEDVDVARIADEALTRARDQLEADCPTDLYLMVGV
                     GAANAGELVVDGRGVAFVCVEHFTGRANPETNGLGLAPHLLPLWIAHEIAHAVRYTSP
                     TSKAEMRRLVAEGEGTYDYWTTGSRATLRELLVNEGIAVAASQAVAPGFEPWEYLGYG
                     RRQYRRIRELDAFLRRAAASELDRRGLGLRLRFLSGGMSPASRLVNGKVLPERSGYYL
                     GLRMVESHVTERGLAATVRAPAEEIQQAEDRASGIQTA"
     gene            1623..1832
                     /locus_tag="AC16JC_10960"
     CDS             1623..1832
                     /locus_tag="AC16JC_10960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165257.1"
                     /translation="MTPRGGAPSERRHNYTLREILEELVGHARDVASRAKSMTPEELE
                     RAQQRLEWLADEVWRVATEQLPPSE"
     gene            1874..>2268
                     /locus_tag="AC16JC_10965"
     CDS             1874..>2268
                     /locus_tag="AC16JC_10965"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR5165258.1"
                     /translation="MHDSGRGFKVSVRKEDLVFAAAHFITLPGHRCESLHGHNYRAGV
                     ELAGALEPDAWYVFDFVALKQVMRRLTNELDHKVLLPTTNPTVTVQANCDSIAVSYDR
                     RPRYVFPAGDCVLLPIPNTTVEMLAEYLAG"
BASE COUNT          346 a          762 c          805 g          355 t
ORIGIN      
        1 gggggaagcg gcaacacgga tctctacgtc aaagcggccc cgggcacggc cgcggccata
       61 ttgcgggaca cggtcgtcga ggcggagttc gcccgggctc agggaggcct gctgcgactc
      121 acggcccccc ccggcgtcta ccggctggcc ctggacgtcg attctgaagg agtcgtcggg
      181 cgggtccgcg agcccgtccg actcccggtc tactcttggg cgtacgtcgg cgtctcgagt
      241 ctcgtgctgg cgacagggga ttcgctgagt gaccgcgagg cggcgcttgc ggcgatgccg
      301 gccgacctca cctatcctgc cggccgcgcg gtcgcgacct acgccgaact gtacggactg
      361 tctcgcgaca gcgacgggcg ggcgcgctac cgcgtccgct acagcttcgc gccactgcgc
      421 tcgttggccg cgcggctgac ccgaggagcc tcaccggtcg tgttcgaatt cgaccgcgag
      481 agcgaatggc gcgggaccat gctggagcga ctggtgatcg agcccgggcg agtgccaccc
      541 ggacggtatc gggtgacgct gtcggtgacc gaccttccga caaatgtcaa gtcggcgacg
      601 gtcgcgctcg acatcaccat ccgctgagcg agacttgtcg gaccgggaga acactcccaa
      661 attggggtgc aacccggtcc aatgttcgtc aatctcgtcc cggaattcct tgcctgcctc
      721 gccgccccgg atccggtcgc ggcttcccac tcgtatctcg aacgtcatgg gccggtgctc
      781 gcgagctact ggcacaacta cgtgctcgat ctggagtcgc cgcacgcgga gcgggtcatc
      841 cgagaggcgg tgcgcgcgga tcgcgacgac ctgcgcagcc tgctcgagga tgtggacgtc
      901 gcgcgcatcg ccgacgaggc gttgacgcgc gcccgggacc agctcgaagc cgactgcccc
      961 accgacctct acctcatggt cggcgtcggc gcggccaacg ccggggagct ggtggtggac
     1021 ggccggggag tcgccttcgt gtgtgtggag cacttcacgg gccgggccaa ccccgagacc
     1081 aacgggctgg gcctggcgcc acatctgctg ccgttgtgga tcgctcatga gatcgcccac
     1141 gcggtacgct acacgtcgcc gacgagcaag gccgagatgc gccggctggt cgccgaagga
     1201 gaaggcacat acgactactg gaccacgggc agccgggcga cgctgcgcga gctgctggtc
     1261 aacgagggca tcgcggtggc ggcctcccag gccgtggctc cgggattcga gccatgggag
     1321 tacctgggct acggccgccg gcagtatcgc cgcatccgtg agctcgacgc tttcctgcgg
     1381 cgtgcggccg cgtcggagct ggatcggcgc ggtctgggcc tgcgcctccg cttcctctcc
     1441 ggcggaatga gcccggcctc ccgcctcgtg aacgggaagg tgctgccgga gcgctcgggg
     1501 tattatctcg gcttgcgcat ggtggaaagc cacgtcacgg aacgcggtct tgcggccacg
     1561 gtgcgggctc ccgcggagga aatccagcag gcggaggacc gcgcgagcgg tatccagacg
     1621 gcatgacccc gcgcggcggc gcgccgtcgg agcggcggca caactacacg ttgcgggaga
     1681 tcctcgaaga gctcgtgggc cacgcgcgcg acgtcgcgag ccgcgcgaag tccatgaccc
     1741 cagaggagtt ggagcgcgcc cagcagcgcc tggagtggct ggcggacgag gtgtggcgcg
     1801 tggcgaccga gcagctgccg ccgtccgagt aaccctgcgg cggcccgggg gccgcgctat
     1861 atcttcccgg cacatgcacg actccgggcg cgggttcaag gtatcggtgc gcaaggaaga
     1921 cctcgtcttc gccgccgcgc acttcatcac gttgccgggc catcgctgtg aatccctgca
     1981 tggtcacaac taccgcgccg gcgtcgaatt ggccggggcg ctcgagccgg atgcgtggta
     2041 cgtcttcgat ttcgtggcgc tgaagcaggt gatgcggcgg ctcaccaacg agctggatca
     2101 caaggtgctg ctgcccacca ccaatccgac ggtcacggtg caggcgaact gcgacagcat
     2161 cgcggtgagc tacgaccgcc ggccgcggta cgtgtttccg gccggcgact gcgtgttgct
     2221 gccgatcccc aacacgacgg tggagatgct cgccgagtat ctcgccgg
//
LOCUS       DBNGZW010000465         2317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1134866, whole genome shotgun sequence.
ACCESSION   DBNGZW010000465 DBNGZW010000000
VERSION     DBNGZW010000465.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1134866"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="AC16JC_10970"
     CDS             <1..693
                     /locus_tag="AC16JC_10970"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5165259.1"
                     /translation="PAPKGLILCSPCNPTGSVYALAELRAIAEWAAARKVWIIADEIY
                     RRIHYGSGPAPSLLDLSDDFLERTVIVTGVSKTYAMTGWRVGVALAPAPVAKAMAALQ
                     SHTTSGANHMAQYAAAAAYADERVEQDVQRMVAEFRRRRDLLVEGFRRRLPGVEFVEP
                     HGAFYFFFRVDSFGGISGTDFCTRLITETGVALVPGGAFGDDRWVRLSYAAATGDIEK
                     ALERIIGFAKKI"
     gene            807..1166
                     /locus_tag="AC16JC_10975"
     CDS             807..1166
                     /locus_tag="AC16JC_10975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5165260.1"
                     /translation="MRRLDSLEAWRQGRNLAREAYRQTLSGPLRNHFSLCDQIRRAAA
                     SIPANIAEGYALSTTPQFLRCLRIALGSATELITHLELAVDMELIGPQAGKEIVATCD
                     REIGLLIGLLRKLGTRR"
     gene            complement(1206..>2317)
                     /locus_tag="AC16JC_10980"
     CDS             complement(1206..>2317)
                     /locus_tag="AC16JC_10980"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR5165261.1"
                     /translation="PALGPEVFARRLDRLRAELDRRKLDLFVAAPSTNFAYYTGASPG
                     RSERLILLLAPAKGDAAIVAPSFEVERVKRASAVPDVRGWDEQDDPWRLVRTALQAMK
                     PAHRVGQGAIEASLDYGSALRLFDVAGGNWKWQSAAPVTERLRLIKYPEEIAQIRRAI
                     EITEASIAATFAALVPGVTEREVAVRLSEEMRTRGAGGGGLVQFGPSSALPHGGPGGS
                     ALQKEMVVLIDAGTQVSGYTSDITRTTWFGDAPADEFKQVFNLVHDAQTAAMQLGKPF
                     TVRCQQMDEAARRVITAGGYGQYFTHRLGHGMGMDGHEPPYLVEGNDTPLEPGMVFTI
                     EPGIYQLDKFGVRIEDDALVTDAGVEVLSQRPAKV"
BASE COUNT          352 a          781 c          791 g          393 t
ORIGIN      
        1 cccgcgccga aggggctgat cctgtgctcg ccatgcaacc cgacgggctc cgtctacgcg
       61 ctggccgagc tgcgtgccat cgccgagtgg gcggcggcgc gaaaggtgtg gatcatcgcc
      121 gacgagatct accgccgtat ccactacggc agcggccccg cgccgtcgct gctggatctt
      181 tccgacgact ttctggagcg cacggtcatc gtgacggggg tcagcaagac ctacgcgatg
      241 acggggtggc gcgtgggcgt ggcgctcgcg ccggcgccgg tggcgaaggc gatggcggcg
      301 ttgcagtcgc acaccacctc cggggcgaat cacatggccc agtatgcggc cgccgccgcg
      361 tacgccgacg agcgtgtgga gcaggacgtg cagcggatgg tggccgagtt ccgccgccgg
      421 cgcgacctgc tggtggaggg cttccgccgc cgattgccgg gggtggagtt cgtcgagccg
      481 cacggggcgt tctacttctt tttccgcgtg gactcgttcg gcggcatctc cggcaccgac
      541 ttctgcacgc gcctcatcac cgagaccggc gtcgcgctgg ttcccggagg agccttcggc
      601 gacgaccgct gggtgcgcct gtcctacgct gcggcgacgg gagatatcga aaaggcgctg
      661 gagcggatca tcggcttcgc gaagaagatc tgaaccaagg ccggggaagg gggaagaggg
      721 aagggggaag ggtgagtgcg ataagatgta caatcgtcgg gtgaatctgg ttacgtgctg
      781 tagcggacca gcgcgcatct tcggggatgc gcaggctcga ctccctcgag gcgtggcgac
      841 agggcaggaa tctggcacgc gaagcgtatc gtcagaccct ctccggtccg cttcgaaatc
      901 atttctcgct ttgcgatcag atccgccgcg ccgcagcgtc gatccccgcg aacatcgcgg
      961 agggatacgc gctatccaca acaccgcaat tcctgcgatg ccttcgcata gcgctcggat
     1021 cggcgaccga gctaatcaca cacctggagc tcgccgtgga catggaactg atcggtcccc
     1081 aggctggaaa ggagatcgtc gctacctgcg accgtgagat cggtctactg attggcctgc
     1141 tgcgcaagct tggtaccagg cggtgaattc ccttccctct tccctcttcc ctcttccccc
     1201 ttcccctaga ccttggccgg cctctgactc aggacctcca cccccgcgtc cgtcacgagc
     1261 gcatcgtcct cgatccgcac cccgaacttg tcgagctggt agatccccgg ctcgatcgtg
     1321 aagaccatcc cgggctcgag cggggtgtcg ttgccttcca cgagataggg cggctcgtgt
     1381 ccgtccattc ccatgccgtg gcccaggcga tgggtgaagt actgtccgta gccgcccgcg
     1441 gtgatgaccc ggcgcgccgc ctcgtccatc tgctgacagc gcaccgtgaa cggcttgccg
     1501 agctgcatcg ccgccgtctg cgcgtcgtgc accaggttga acacctgctt gaactcatcg
     1561 gcgggcgcgt cgccgaacca ggtggtgcgc gtgatgtcgg acgtatagcc cgagacctgg
     1621 gtgccggcgt cgatcagcac caccatctcc ttctggaggg cggagccgcc ggggccgccg
     1681 tggggcaggg cgctggaggg tccgaactgg accagcccgc cgccgcccgc gccgcgcgtg
     1741 cgcatctcct cgctgagcct gacggccacc tcgcgctcgg tgaccccggg gacgagggcg
     1801 gcgaaggtgg cggcgatcga cgcttcggtg atctcgatgg cccggcggat ctgagcgatc
     1861 tcttcgggat acttgatgag ccgcagccgc tcggtgaccg gcgcggcgct ctgccacttc
     1921 cagttcccgc ccgcgacgtc gaacagccgg agtgccgacc cgtagtccag gctcgcttcg
     1981 atagccccct ggccgacccg gtgcgcgggc ttcatggcct gcaacgccgt ccgcacgagc
     2041 ctccagggat cgtcctgctc gtcccagccg cgcacgtcgg gcacggcact ggcgcgcttc
     2101 acgcgctcca cctcgaagga cggtgcgacg atcgcagcat cgcccttcgc gggcgcgagc
     2161 agcagaatca ggcgctcgct gcgtcccgga gaggcgcccg tgtagtaggc gaagttggtg
     2221 ctcggggcgg cgacgaacag atcgagcttg cgccggtcga gctcggcgcg cagccggtcg
     2281 aggcggcggg cgaagacttc cggtcccaag gccggtt
//
LOCUS       DBNGZW010000466         2747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_588458, whole genome shotgun sequence.
ACCESSION   DBNGZW010000466 DBNGZW010000000
VERSION     DBNGZW010000466.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_588458"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="AC16JC_10985"
     CDS             complement(<1..477)
                     /locus_tag="AC16JC_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HIR5165262.1"
                     /translation="MTPRVTQDAYTPRPEHHFTFGLWTVGNPGRDPFGEPVRQPLKPT
                     DSVRKLAELGAYGVNLHDEDLVPRDAGAAERDRIVREFRQALSDSGMKVPMATTNLFS
                     DPAFKDGAFTSNDARVRAYALQKTMAAIDLGVELGATIYVCWGGREGVETNAAKDPR"
     gene            546..1607
                     /gene="galT"
                     /locus_tag="AC16JC_10990"
     CDS             546..1607
                     /gene="galT"
                     /locus_tag="AC16JC_10990"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HIR5165263.1"
                     /translation="MVWEQRWHPLRREWVVVSSHRNERPWLGERVVEPPRALPTYVPD
                     CHLCPGNARSSGRRNEPYRGVFVFDNDHPCVGADAPTSLGKPAGIYRNQPALGCARVV
                     CFTPRHDLTLARLGGAEIVTLLEVLQAQYRELGGRDEVRHVLVFENKGEVTGVSNPHP
                     HCQIYATNFVFKTIELEAEAQAAHLTEHGRPLFQDILSAEEADGRRLLVRRDTALSFI
                     PYFARYPYEMFVAPRATRPSLADLTPPELADFAGVLRETLIRLDNLWRTAFPYVMVLH
                     QAPTKGQPSPGFHFHIQIHPPLRKPGLLKYLAGPEIGGGNFLNDAAPEEKAAELQAVS
                     SVHYTREGEGGRGKGEGLE"
     gene            1604..2605
                     /locus_tag="AC16JC_10995"
     CDS             1604..2605
                     /locus_tag="AC16JC_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5165264.1"
                     /translation="MTAAESLLEPLLSLHDRVRTAVVDACARAAEDELAAVAADETAG
                     GGDTIYAVDRVAEEVFIEGLADLARKEPLCVVAEGLPENALGEDGRWRLLVDPIDGTR
                     GLMYQKRSAWILTGIAPNRGRDTRLRDIVLAVQTEIPLLKQHLSDQLWAIRGQGGGAS
                     ARRVNRLSGARASLTLRPSRADTVAHGFATVVRFFPGARDVLAAIDDDVVQALVRPSS
                     GRAACFEDQYASTGGELYELLSGHDRFIADLRPLMQSVRAARGLAAGLCCHPYDLCSS
                     LIAEAAGVILTDPGGAALDAPFDVTSDVAWVGYANERLRAQVEPVLQDALRRRGLLP"
BASE COUNT          427 a          945 c          936 g          439 t
ORIGIN      
        1 gcgcgggtcc ttggccgcgt tggtctcgac cccttcccgc ccaccccagc agacgtagat
       61 ggtggcgccg agctccaccc ccaggtcgat cgcggccatg gtcttctgga gcgcgtacgc
      121 ccggacccgc gcgtcgttgc tggtgaacgc gccgtccttg aacgcggggt cggagaagag
      181 attagtggtc gccatcggca ccttcatccc ggagtccgag agtgcctggc ggaactcccg
      241 gacgatccgg tcgcgctccg ccgcgcccgc gtcgcgaggc acgaggtcct cgtcgtggag
      301 gttgacgcca taggcgccga gctccgcaag cttccgcacg ctgtctgtgg gcttcagcgg
      361 ttgccgcacc ggctcgccga agggatcgcg gccgggattc cccaccgtcc agagcccgaa
      421 ggtgaagtgg tgctccggcc tgggcgtata cgcgtcctgc gtgactcgtg gcgtcatcgg
      481 ttgggtcccc ggctcgattc ttgagacttg ccgccctgtc gcgcggagac catactcgcg
      541 cgaccatggt ctgggaacag cgctggcatc cgctgcgacg cgagtgggtc gtcgtgtcct
      601 cccatcgcaa cgagcggccc tggctgggcg agcgtgtggt cgagccgccg cgcgcgctgc
      661 ccacctacgt gccggactgc cacctctgtc cggggaacgc gcgcagctcg gggcggcgca
      721 acgagcccta ccgcggcgtc ttcgtgttcg acaacgacca cccgtgcgtc ggcgcggacg
      781 cccctacatc actcggaaag ccagcgggaa tctaccggaa tcagcccgcg ttggggtgcg
      841 cccgggtcgt ctgcttcacg ccgcggcacg acctgacgct cgcccgactc ggcggcgccg
      901 agatcgtgac actcctggag gtgctccagg cgcaataccg ggagctgggc ggacgcgacg
      961 aggtgcgcca cgtgctggtg ttcgagaaca agggcgaggt cacgggcgtg agcaaccccc
     1021 acccgcactg ccagatctac gcgacgaact tcgtgttcaa gacgatcgag ctcgaggccg
     1081 aggcgcaagc agcgcacctg acagaacacg gacggccact gttccaggat atcctctccg
     1141 ccgaggaagc ggacggccgg agactgctgg tgcggcgtga cacggcgctg tcgttcatcc
     1201 cgtatttcgc ccgctatccg tacgagatgt tcgtcgcacc gcgcgccacg cgccccagcc
     1261 ttgccgacct tacgcccccc gagctcgccg acttcgccgg cgtcctgcgc gagaccctca
     1321 tccggctcga caacctgtgg cgcacggcgt tcccgtacgt gatggtgctg caccaggcgc
     1381 ccaccaaggg acagccgtct ccgggatttc acttccacat ccagattcat cccccgctgc
     1441 gcaagccggg cctcctcaag tatctcgccg gcccggagat cggcggcggc aacttcctca
     1501 acgacgcggc ccctgaggag aaggcggctg agcttcaggc cgtctcgagc gtccattaca
     1561 cgcgggaggg ggagggggga agggggaagg gggaagggtt agaatgaccg cagcggagag
     1621 cctgctcgag ccactgctct ccctgcacga tcgcgtccgg accgcggtgg tggacgcatg
     1681 cgccagggcg gcggaggacg agctcgcggc cgtggcggca gatgagaccg ctggaggcgg
     1741 cgacaccatt tacgcggtcg accgcgtcgc cgaggaagtc ttcatcgaag ggctcgccga
     1801 cctggcgcgc aaggaaccgc tctgcgtggt cgccgaaggc cttccagaga acgcgttggg
     1861 agaagacggc cgctggcgcc tccttgtcga cccgatcgac ggcacgcgcg gcctgatgta
     1921 tcaaaagcgg agcgcgtgga ttctgaccgg catcgcaccg aatcgcggtc gcgacacacg
     1981 gttgcgcgac atcgtgctcg ccgtgcagac cgaaatcccg ctcctgaagc agcatctctc
     2041 cgaccagctc tgggcgattc ggggtcaggg agggggggca tcggcacggc gggtcaaccg
     2101 gctgtcgggc gcgcgcgcga gcctcacgct acgcccttcg cgcgccgaca ccgtcgcgca
     2161 cggcttcgcg acggtcgtgc gattcttccc gggagcccgc gacgtgctgg ccgccatcga
     2221 cgacgacgtc gtgcaggcgc tggtgcgccc ctcgtccggg cgggctgcct gcttcgaaga
     2281 ccagtacgcg tccaccggtg gcgagctgta cgagctgctg tccggtcatg accgcttcat
     2341 cgcggacctg cggccgctga tgcagtcggt ccgcgccgcg cggggattgg cggccgggct
     2401 ttgttgccat ccctacgatc tctgctcgtc tttgatcgcc gaggcagcgg gcgtgatcct
     2461 caccgatccc ggcggtgccg cgctcgacgc gccgttcgac gtgaccagcg acgtggcgtg
     2521 ggtgggctac gcgaacgagc ggctccgcgc ccaggtcgag ccggtgttgc aggacgcgct
     2581 gcggcggcgt ggactactac cgtagaccga gaccagatcc gaccagatcc ctcgcgcctg
     2641 cggcgctcgt gatgacaagg gcgtctgaca tctaaataga aagcaaagac gaagtgattc
     2701 cactccggag ccagcccgac gttcgcactg ctgatgacct tgtctgg
//
LOCUS       DBNGZW010000467         2996 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1612451, whole genome shotgun sequence.
ACCESSION   DBNGZW010000467 DBNGZW010000000
VERSION     DBNGZW010000467.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2996)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2996)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2996
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1612451"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(202..1602)
                     /locus_tag="AC16JC_11000"
     CDS             complement(202..1602)
                     /locus_tag="AC16JC_11000"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR5165265.1"
                     /translation="MSATGRVSQTFRTTLRRVVRLALPVVAIQLGWMMMGVVDTIVVG
                     HVSADAMAAVALGNLWFLAVSIFGMGVLMGLDPIVAQAVGAGEEAAVARALQRGVLLV
                     VLLTVLCSVALLPAEPLFSVLRQPAEIVPTAAAYVRIAVGGVLPFLLVVLVRSVMQAT
                     GEVAPMVWATLAANLLNLMLNWVLVFGHLGLPALGAIGSSWATTLSRWALAALMIAFA
                     WPRLRHRLLPLRREALAFAPLTRILALGAPIGLQYEVELGIFSLVGIMMGQLGAAPMA
                     ANQVALNLVSVTFMVPLGVSMAAAVLVGQAVGRGDIAEVRRTGTAALVCGVGFMAASG
                     MLMLAFPRVLAHVYTIDPSVAAIAVTLIPAAGAFQVFDGTQVVSIGILRGTGDTRTPL
                     FVHVLGYWAVALPLATWLGMHTTAGPAGMWWGLVVGLAVMAGALVVRVRRRLRGPLQR
                     VVVDHPAGEETADRVT"
     gene            complement(1677..2498)
                     /locus_tag="AC16JC_11005"
     CDS             complement(1677..2498)
                     /locus_tag="AC16JC_11005"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR5165266.1"
                     /translation="MKISRGRGYAMLVLLTLTAPALTAQSDADGWLAECRRGHWNERP
                     HVCRLRETGMRPMSGALTVDPGENGAIAIYGWDRDSIAVVAKIEAGARSEEDARQLAD
                     AVRIDAAGGTIRASGPATGWRQQWSVSFEVYVPRHTDLALTTENGPISIADVTGTMHL
                     RAQNGPVSLSGVGGDITARVQNGPLTVELSGTTWDGAGLDAEARNGPVDLAIPERYNA
                     QLETGTVNGPMDVAFPLTVTVQGHVTNRIRTTLGTGGAPLRIVTTNGPLTIRRAH"
     gene            2696..>2996
                     /locus_tag="AC16JC_11010"
     CDS             2696..>2996
                     /locus_tag="AC16JC_11010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165267.1"
                     /translation="MRLSNLEQSISKHSRTLHLDSIRSRILLFAVLATLIPSIATAWI
                     AYSHLQQSLTDKVTQEVSGAAKQSGHAVAQWLKDRVYDLRVFASSYVVTENLEEMR"
BASE COUNT          518 a         1076 c         1001 g          401 t
ORIGIN      
        1 gaacggcgcg ggggcgatgt ctggagcagc tgcggcacgc gggatggcgg cgccgcccgc
       61 cgcgggccgc acgacgccgg cgatggttcc gagccctgca tactggatga actcccgacg
      121 agacagatac ggcatggagc actccggaag gtcggatggt ccggaagaat gtgcacgtcg
      181 ttgccccgcg cggctacgag gtcaggtgac gcggtccgcc gtttcctccc ccgcgggatg
      241 gtccacgaca acccgctgga gcggaccgcg caggcgccgc cgcacgcgga ccaccagcgc
      301 cccggccatg accgcgagcc cgacaacgag cccccaccac atccccgccg gcccggcagt
      361 cgtgtgcatg ccgagccagg tggccagcgg caacgcaacc gcccagtatc cgaggacatg
      421 gacgaagagc ggcgtgcggg tatctccggt gccgcgcagg atgccgatgg agacgacctg
      481 cgtcccgtcg aatacctgaa aggcacccgc ggccgggatc agcgttacgg cgatggctgc
      541 caccgacggg tcgatcgtgt agacgtgggc gaggacccgg gggaaggcga gcatcagcat
      601 cccgctcgcc gccatgaagc ccaccccgca gaccaacgcc gccgtcccgg tgcgccgcac
      661 ctcggcgatg tccccgcgcc ccaccgcctg gcccacgagg acggccgccg ccatcgagac
      721 cccgagtggc accatgaacg tcacggacac gaggttcagc gccacctgat tcgcggccat
      781 gggagcggcg ccaagctgtc ccatcataat cccgacgagg ctgaagattc cgagttccac
      841 ctcgtactgc aacccaatcg gggcgccgag cgcgaggatg cgggtcagcg gggcgaaggc
      901 gagcgcctca cggcgcagcg gcagcaggcg gtggcgcaac cgtggccagg cgaaggcgat
      961 catgagggcg gccagtgccc agcgcgacag cgtcgtggcc cagctggagc cgatggcccc
     1021 caacgcgggc aacccgaggt gcccgaacac caggacccag ttcagcatca agttcaggag
     1081 attcgccgcc agggtcgccc agaccatggg ggcgacctcg ccggtcgcct gcatcactga
     1141 gcgtaccagg accacgagca ggaacggcag cactccgccc acggcgatcc tgacataggc
     1201 cgccgcggtg ggaacgatct ccgcgggctg gcgcaggacc gagaacagcg gttcggccgg
     1261 gagcagcgcg acgctgcaga ggaccgtcag gagcacgacc agcagcactc cccgctgcag
     1321 cgcgcgagcg acggcggcct cctccccggc cccgaccgcc tgggcgacga tcgggtcgag
     1381 acccatgagg acgcccatgc cgaagatcga gacggcgagg aaccacagat tgccgagcgc
     1441 gaccgccgcc atggcatcgg ccgagacgtg cccgacgacg atggtgtcca cgacgcccat
     1501 catcatccag cccagctgga tggccaccac cgggagcgcg agacgcacca cgcggcgcag
     1561 cgtcgtgcgg aacgtctggg acacgcggcc ggtcgcggac atcccggaaa ctaccgtcgc
     1621 acgacacgcg cgccgagtcc accggacccg gcgcgcgtgc ggaaacgatg aagccgctag
     1681 tgggcccggc gaatcgtcag cgggccgttc gtggtgacga tgcgcagcgg cgcaccgccc
     1741 gtgccgagcg tggtgcggat acgattcgtc acgtggccct gcaccgtcac agtcagcggg
     1801 aaggcaacgt ccatcgggcc gttcacggtc cccgtctcga gctgggcgtt gtagcgctcg
     1861 gggatcgcca ggtcgaccgg accgttgcgc gcttccgcat cgagcccggc gccgtcccac
     1921 gtggtgccgg acagctccac cgtcagcggc ccgttctgaa cccgggcggt gatgtccccg
     1981 cccacaccgc tgagcgacac ggggccgttc tgcgcacgca gatgcatcgt ccccgtcacg
     2041 tccgcgatcg agatcggacc gttctcggtc gtcaacgcga gatcggtgtg ccgcggcacg
     2101 tatacctcga agctcaccga ccactgctgc cgccacccgg tcgccggacc ggacgcgcgg
     2161 atcgtgccgc ccgccgcgtc gatgcgcacc gcgtccgcga gctgccgtgc gtcttcctcg
     2221 ctccgcgcgc cagcctcgat tttcgccacg acggcgatcg aatcccgatc ccatccgtag
     2281 atggcgatgg cgccgttttc tcccgggtcc accgtcaatg cgccgctcat cggccgcatc
     2341 ccggtctccc ggaggcggca gacatggggc cgttcgttcc agtgaccgcg ccggcactcg
     2401 gccagccacc cgtcggcgtc ggattgtgcg gtcagcgccg gagccgtcag ggtgaggaga
     2461 accagcatcg cgtagccacg gccacggctg atcttcatgg atgtctcgtc gtaggggtcc
     2521 cccgttggac acgaccccgt gccggaaggt tgccccgacg tgctccaggc ccgcccaagg
     2581 cggtgagcgg gctcacaacc tgttcgggcg tttgcccttg caccgatgcg ccaaggcacc
     2641 ctacttgcgg ttggcaggac agggtgaatc attcacggat tggcttgggg actggatgcg
     2701 actgagcaac ctggaacaat cgatttcgaa gcattcccgg acgctgcatc tcgacagcat
     2761 ccggagtcgc attctgctgt ttgccgtcct ggcgaccctc atcccgtcca tcgccaccgc
     2821 ctggatcgcc tactcccacc tgcagcagtc cctgaccgac aaggtcaccc aggaggtgtc
     2881 cggggcggcc aaacagagcg gccacgccgt cgcgcagtgg ctgaaggacc gggtctacga
     2941 cctgcgcgtg ttcgccagct cctacgtcgt gaccgagaat ctcgaggaga tgagac
//
LOCUS       DBNGZW010000468         2025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1135064, whole genome shotgun sequence.
ACCESSION   DBNGZW010000468 DBNGZW010000000
VERSION     DBNGZW010000468.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2025
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1135064"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..470)
                     /gene="tsaA"
                     /locus_tag="AC16JC_11015"
     CDS             complement(39..470)
                     /gene="tsaA"
                     /locus_tag="AC16JC_11015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HIR5165268.1"
                     /translation="MTDTPSGYTVQPIGRVHSILRRRADAPRQGDEGAPDARIRVDPA
                     FRPGLDGIAAGQDILILTWLHEARRDLLAVHPRGDRTQPLAGVFATRSPDRPNPIGLH
                     RVKVLAVAADGCLHVQGLEAIDGTPVIDLKPVLHPTTADGA"
     gene            complement(481..921)
                     /locus_tag="AC16JC_11020"
     CDS             complement(481..921)
                     /locus_tag="AC16JC_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002616252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5165269.1"
                     /translation="MTANTATQSLALEYDLPHPPAKVWRALTDPGLLAQWLMANDMRA
                     TVGHAFTFTQDPTPWWDGVVRCEVLESEPPKRLRYSWRSGPESSTLDTVVTWTLTPTA
                     AGGTRLALEQTGFQPGNRFAFEGARKGWERMVGEGLTQLLARAE"
     gene            complement(918..1235)
                     /locus_tag="AC16JC_11025"
     CDS             complement(918..1235)
                     /locus_tag="AC16JC_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012238929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5165270.1"
                     /translation="MPREDTVFRALADPSRRAIFERLTRGEAAVKDITARFDLSQPAV
                     SQHLATLRRAGLVSERRQGRLVYYRVKRDGLRPLIDWIAHYQAFWLERLDRLHALLKE
                     MDE"
     gene            complement(1339..1629)
                     /locus_tag="AC16JC_11030"
     CDS             complement(1339..1629)
                     /locus_tag="AC16JC_11030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010495659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR5165271.1"
                     /translation="MPPQEPKGPFCQSCSMPLGKPDDFGTDQAGYRVNDYCRHCYADG
                     AFTEPAISMEAMLARCVAVMDTQGIMPAPQARALLADVLPRLKRWRTPVGAR"
     gene            complement(1831..>2025)
                     /locus_tag="AC16JC_11035"
     CDS             complement(1831..>2025)
                     /locus_tag="AC16JC_11035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165272.1"
                     /translation="QTLGTVRQVLDFAELESPAGFIRDVERTTIHDMTQRWRKDLSPE
                     QQALLTDVLREDLLRYGYEL"
BASE COUNT          292 a          694 c          727 g          312 t
ORIGIN      
        1 cagctcctcc tggtgcgcct cgctcccggc cggattcctc aggcgccgtc cgcggtcgtc
       61 gggtgcagga ccggcttcag atcgatgacc ggcgtcccgt cgattgcctc gagcccttgc
      121 acgtgcaggc agccgtccgc ggcgaccgcc agcaccttga cccgatgcaa tccgatcgga
      181 ttgggacggt ccggcgagcg cgtggcgaag acgccggcca gcggctgcgt ccggtctccg
      241 cgcggatgca ccgcgagcag gtcccgtcga gcctcgtgca gccatgtcag gatcagaata
      301 tcctgtccgg ccgcgatccc gtcgagcccc gggcggaagg ccgggtcgac ccggatccgc
      361 gcgtcgggcg caccctcgtc tccctgcctc ggcgcgtcgg cgcgccgccg caggatggag
      421 tgcacccggc cgatcggttg cacggtgtac cccgacggcg tgtccgtcat cgtgccgctg
      481 tcactcggcc cgcgccagca gttgggtcag gccctcgccg accatgcgct cccatccctt
      541 cctggcgccc tcgaacgcga agcggttgcc gggctggaag ccggtctgct ccagcgcgag
      601 gcgggtgccg cccgccgccg tcggcgtgag cgtccaggtg accacggtat ccagcgtcga
      661 cgactcgggc ccgctccgcc acgagtagcg gaggcgcttg ggcggctcgc tctccagcac
      721 ctcgcagcgc acgaccccgt cccaccacgg cgtcgggtcc tgggtgaagg tgaaggcgtg
      781 cccgaccgtg gcccgcatgt cgttggccat gagccactgc gccagcagcc cgggatcggt
      841 cagcgcgcgc cagaccttgg cgggcgggtg cggcaggtcg tactcgaggg cgagcgactg
      901 ggtggcggtg ttggcggtca ttcgtccatc tccttgagca gtgcgtggag ccggtcgagg
      961 cgctcgagcc agaacgcctg gtagtgagcg atccaatcga tgagcggccg gagcccgtcg
     1021 cgcttcaccc gatagtagac caggcggccc tggcgccgct ccgagaccag gcccgcgcgg
     1081 cggagcgtgg ccaggtgctg ggagacggcc ggctgggaga ggtcgaagcg cgccgtgatg
     1141 tccttgacgg cggcctcgcc ccgggtgagg cgctcgaaga tggcgcggcg gctggggtcg
     1201 gccagcgcgc ggaagacggt gtcttcgcgt ggcatggcaa atatataagc agttacttat
     1261 ggaattgtca atctcgcaaa cgcggacagg gccccggcga cctcggtcac cggggccctc
     1321 gagcgtggtg cgatcgcgct accgggcgcc gaccggggtc ctccaccgct tgaggcgcgg
     1381 caggacgtcg gccagcagcg cgcgggcctg aggggccggc atgatgccct gcgtgtccat
     1441 cacggccacg caccgggcca gcatggcttc catcgagatg gcgggctcgg tgaacgcgcc
     1501 gtcggcgtag cagtggcggc agtagtcgtt cacccggtag ccggcctggt cggtcccgaa
     1561 gtcgtcaggc ttgccgagcg gcatggagca gctctgacag aaggggccct tgggttcctg
     1621 gggcggcacg tgatcctccc tccggcggag gtgaaggcga tccgagatct gcggacgcca
     1681 cagcttagga ccacgaccag taagggaaaa tgaagccgag gtgagactgt tcgaatccca
     1741 tgtcgtccct ctgccattca gcgaactcaa ccggtcagct gtgcgacggc acgacgacgg
     1801 cccggctcaa gcctacggcc cgggccgagc tcagagctcg tagccgtacc gcagcagatc
     1861 ctcgcgcagc acgtcggtga gcagggcttg ctgctccggg ctcaggtcct tgcgccaccg
     1921 ctgggtcatg tcgtggatcg tcgtccgctc gacgtcgcgg atgaagccgg ccggactctc
     1981 gagctccgcg aagtccagca cctgccggac ggtgccgagg gtctg
//
LOCUS       DBNGZW010000469         4023 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1204618, whole genome shotgun sequence.
ACCESSION   DBNGZW010000469 DBNGZW010000000
VERSION     DBNGZW010000469.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1204618"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="AC16JC_11040"
     CDS             complement(<1..866)
                     /locus_tag="AC16JC_11040"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5165273.1"
                     /translation="MNYPSVVRGAWLTVALAAACTKSKGGAPGGGAGGGPPAFPVEVA
                     VARTDTVRDEIVATGQVEALQAIELHPEVEGRIVAILFREGQEVATGTPLFQIDSTEL
                     AAQVAQLAAQRDMARQDLARTRELIAQNAASPSDLEHAEAAARSADAQYRLQSTRLAR
                     TTVRAPFGGTVGQRFVSLGDYVDNGHRLVSLQTVNPQRASFQVPERFARALKRGQRVT
                     FRVAAIAGREFTGEVDFVDPVVQLPGRTILVKARVPNPQRLLQAGMFIEARLVTALRP
                     KAVVIPEDAIVP"
     gene            complement(863..2152)
                     /locus_tag="AC16JC_11045"
     CDS             complement(863..2152)
                     /locus_tag="AC16JC_11045"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR5165274.1"
                     /translation="MILPLLAVLQVTSAQDSVPVVTLAEALQRAARLDPGYVTAAGQV
                     NNAEWARRAAFAVFIVPAVTLSANKSHYWPPFFNFATFTAVPNTYSATIDARIDLFTG
                     GQKLAGLAASGAALEGAHAGELQARLTTAMNTELDYYGVLAQRALERVAEDQLQRAQQ
                     QFAVARARVTSGAAVQTDSLQLALGLTQARVVLLQQQAALRAAQLQLGRRIGLEGPAD
                     AAPVPADSAAAPTLPFTVADAVARALAQGPAWRIARANERAASAELWARRGDYLPHAS
                     LDFTGGAFDTRFYPQAIKRSSLSLTVTFPLWNNGQREIQLSQARVTRDVARAIRTDLE
                     RAARHDVTVAYDAYETSRATFALQRDAVVVARENNRVQETRYRAGATTILDLLDAQNR
                     LVSAEADLVQATYNTRLARAGLEVILGERLTSDQGSP"
     gene            complement(2149..>4023)
                     /locus_tag="AC16JC_11050"
     CDS             complement(2149..>4023)
                     /locus_tag="AC16JC_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5165275.1"
                     /translation="ENAYRHQEELHEAPERAAINGTNEIGFAVIATTIALVAVFTPLA
                     FLQGTTGRLFNEFGIAMAGAVIISGFVALTLTPMLSAKILRVSHEHGAVFKLFERGFN
                     AIAQRYATLLRSALRHRGLVVAGTVGSVLLAFVLYKNLKQELLPDDDRGFFLVVSSAP
                     EGASLEYMDGYVRQVEQIIGKTPDVQGYFTIIGGFSGGVNRSFVGVILKDWSVRHTSV
                     QQMVGGLFPQLFGIAGVRAFPYSPGALGFSQPIQFVVQNPDFDRLAQAMDTLLPRASA
                     IKGLVNVDTDLRVNKPELRVTFDRDRAEDLGVPVRDVAAALQSFLGGRRVSTFTRNDK
                     LYYVMVQLDPSRRATPSDMSGIYLRGNNGQLVQLSALAQVEEGVGPRQINHFNRVPSF
                     TLSASLVPPFAQGEALDSLERLARRVLPPGSTTELAGDSREFRESGGALYFAFGVALL
                     VVFMVLASQFESLVHPFTVLLAVPLAVTGALATLLVAALFHRPGGTINLYSEIGMILL
                     IGLVTKNSILLVEYSNQLKRRGLDTMAAVIEAGRIRLRPILMTSVSTIMGAIPVMIGL
                     GAGSTSRRPLGYAIVGGIFFSTALTLFVVPVAYTLLDRLRAREEHDLQQTALEPAEAK
                     "
BASE COUNT          633 a         1425 c         1384 g          581 t
ORIGIN      
        1 aacggcacga tggcgtcctc ggggatgacc accgccttgg gccgcagcgc cgtgaccagc
       61 cgggcctcga tgaacatgcc cgcctgcagc aggcgttgcg ggttgggcac acgcgccttg
      121 accaggatcg tgcggccggg gagctgcacc acgggatcca cgaagtccac ctcgcccgtg
      181 aactcccgcc cggcgatggc ggcgacgcgg aacgtcacgc gctgcccgcg cttgagcgcc
      241 cgcgcgaacc gctccgggac ctggaacgag gcccgctgcg ggttgacggt ctgcagcgac
      301 acgagccggt ggccgttgtc gacgtagtcc cccaggctca cgaaccgctg gcccacggtc
      361 ccgccgaacg gggcccgcac cgtggtgcgt gcgagccggg tgctctgcag ccggtactgg
      421 gcgtcggcgc tgcgcgcggc ggcctcggcg tgctcgaggt cggacgggga ggcggcgttc
      481 tgggcgatca gctccctggt gcgcgccaga tcctgtcgcg ccatgtcgcg ctgcgcggcc
      541 agctgcgcca cctgcgcggc gagctccgtg gaatcgatct ggaacagcgg cgtccccgtc
      601 gcgacctcct gtccctcacg gaacagaatg gcgacgatgc gcccctccac ttcggggtgc
      661 agctcgatcg cttgcagcgc ctcgacctgc cccgtggcca cgatctcgtc gcgcacggtg
      721 tcggtacgcg ccaccgcgac ctcgacgggg aatgccggcg gaccgccgcc ggccccgccc
      781 cctggtgctc cacccttgct cttggtgcac gccgcggcca gcgccaccgt cagccacgca
      841 ccacgtacca cggacggata gttcacggtg atccctgatc ggaggtgagc cgctcgccga
      901 ggatgacttc caggcccgcc cgggcgagcc gggtgttgta ggtcgcctgc accaggtcgg
      961 cttccgccga caccagccgg ttctgcgcgt cgaggagatc gagaatggtg gtcgccccgg
     1021 cgcgataccg ggtttcctgg acgcggttgt tctcccgggc cacgacgacg gcatcgcgct
     1081 gcaacgcgaa ggtggcccgg ctggtctcat acgcgtcgta cgccaccgtc acgtcgtggc
     1141 gggccgcgcg ctcgagatcg gtccgaatcg cccgggcgac atcgcgggtc acgcgggcct
     1201 gcgacagctg tatctcccgt tgtccattgt tccacaacgg gaacgtcacg gtcagcgaca
     1261 ggctcgaccg cttgatcgcc tggggataga acctggtgtc gaatgcgccg ccggtgaagt
     1321 cgagcgacgc atgcggcagg tagtccccac ggcgggccca gagctccgcg gacgccgccc
     1381 gctcgttggc tcgcgcgatg cgccacgccg gaccctgcgc cagcgcgcgg gccaccgcat
     1441 ccgcaaccgt gaacggcagt gtgggagcgg cggcactgtc ggcgggcacc ggggccgcat
     1501 ccgccgggcc ttccagaccg atgcgccggc ccagctggag ctgcgcggcc cgcaacgccg
     1561 cctgttgctg cagcagaacg acgcgcgcct gcgtcaaccc gagcgcgagc tgcagcgagt
     1621 cggtctgcac cgccgccccg gacgtgacgc gcgcccgcgc caccgcgaac tgctgctggg
     1681 cgcgctgcag ctgatcctct gccacgcgct ccagcgcccg ttgcgccagg acgccgtagt
     1741 agtccagctc ggtgttcatc gcggtcgtca ggcgcgcctg cagctcgccc gcgtgggcgc
     1801 cctcgagcgc ggcgccgctc gccgccagcc cggcgagctt ctgtcccccg gtgaacaggt
     1861 cgatgcgggc atcgatcgtc gccgagtagg tgttcgggac tgccgtgaag gtggcgaagt
     1921 tgaagaacgg cggccagtaa tggctcttgt tggccgacag cgtcacggcc ggcacgatga
     1981 aaacggcgaa cgccgcgcgg cgtgcccact cggcgttgtt cacctgcccc gcggccgtga
     2041 cgtaaccggg atcgagacgc gcagcccgct gcagcgcctc ggcgagcgtc accaccggca
     2101 ccgaatcctg ggcagacgtc acctgcagga cggcgaggag cggcaggatc acttcgcctc
     2161 cgcgggctcg agggcggtct gctgcaggtc gtgctcctcg cgggcgcgca gccggtcgag
     2221 cagcgtgtag gcgaccggta cgacgaacag cgtcagcgcc gtggagaaga agatgccgcc
     2281 cacgatcgcg tagcccagcg gccggcggga ggtcgagccc gccccgagtc cgatcatcac
     2341 cgggatcgcg cccatgatgg tcgacaccga cgtcatcaga atcggccgga ggcgaatgcg
     2401 ccccgcctcg atcacggcag ccatggtgtc cagtccccgc cgcttgagct ggttcgagta
     2461 ctcgaccagc agaatggagt tcttggtcac cagcccgatg agcagaatca tgccgatttc
     2521 gctgtacaga ttgatggtgc ccccgggacg atggaacagc gcggccacga gcagggtcgc
     2581 cagcgctccg gtcaccgcca gggggaccgc caggagcacc gtgaacgggt gcaccagcga
     2641 ctcgaactgc gacgccagca ccatgaacac gaccagcagg gcgacgccga acgcgaagta
     2701 caacgcgcct ccgctctccc tgaactcgcg cgaatcgccc gccaattcgg tcgtgctgcc
     2761 cggcggcagc actcgcctgg ccaaccgctc gagcgaatcg agggcctcac cctgggcgaa
     2821 gggcggcacc agcgaagccg acagcgtgaa cgagggcacc cggttgaagt ggttgatctg
     2881 ccgggggccc acgccctcct cgacctgggc gagggccgac agctggacca gctgcccgtt
     2941 gttgccgcgg aggtagatcc ccgacatgtc gctcggcgtg gcccggcggc tgggatcaag
     3001 ctgcaccatc acgtagtaca gcttgtcgtt ccgcgtgaag gtgctgacgc gccggccgcc
     3061 gaggaacgac tgcagggcgg cggcgacgtc gcgcaccggc acgcccagat cctcggcgcg
     3121 gtcccggtcg aaggtgacgc gcaattcggg cttgttgacg cgcaggtccg tgtccacgtt
     3181 cacgagaccc ttgatcgcgc tcgcgcgcgg cagcagcgtg tccatcgcct gggcgagccg
     3241 gtcaaaatcc gggttctgca cgacgaactg gatcggctgg ctgaacccga gcgctccggg
     3301 gctgtagggg aacgcccgga cgcccgcgat cccgaagagc tgggggaaca gcccgcccac
     3361 catttgctgc acgctggtgt gccgtacgct ccagtccttg aggatcaccc cgacgaacga
     3421 ccggttcacg ccccccgaga agccgccgat gatcgtgaag tatccctgga cgtcgggggt
     3481 cttgccgatg atctgctcga cctggcgcac atacccgtcc atgtactcga gcgaggcccc
     3541 ctcgggcgcc gacgacacca ccaggaagaa tccgcggtcg tcgtccggca gcagctcctg
     3601 tttgaggttc ttgtacaaca cgaacgcgag cagcactgac ccgaccgtcc cggcgacgac
     3661 gaggccccgg tgccgcagcg cggaccgcag caaggtcgcg tagcgctggg cgatggcgtt
     3721 gaacccgcgc tcgaacagct tgaacacggc gccgtgctcg tgcgagacgc gcaggatctt
     3781 ggcactcagc atcggggtga gagtcagcgc cacgaagccg gaaatgatca ccgcgccggc
     3841 catcgcaatg ccgaattcgt tgaacagccg cccggtggtg ccctgcagga aggcgagcgg
     3901 cgtgaacacc gccacgagcg cgatggtggt cgcgatcacg gcgaagccga tctcgttcgt
     3961 cccgttgatg gcggcgcgct ccggcgcctc gtgcagctct tcctggtggc ggtaggcatt
     4021 ctc
//
LOCUS       DBNGZW010000470         2831 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_929820, whole genome shotgun sequence.
ACCESSION   DBNGZW010000470 DBNGZW010000000
VERSION     DBNGZW010000470.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2831)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2831)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2831
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_929820"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(220..798)
                     /locus_tag="AC16JC_11055"
     CDS             complement(220..798)
                     /locus_tag="AC16JC_11055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002203032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HIR5165276.1"
                     /translation="MTTRRSIFVSAAVVGVAVLWYLFRPDALVISKTVSEPFPAENHS
                     MSARPETPGMTMPASAMTDSPAMAHSGLSAAEPVLVTTGRFHKNAHETTGAATIYRLA
                     DGRLVLRLTEFATSNGPDVRVYLVAAADVQDEAAAKRAGFVDLGALKGNIGDQNYDLP
                     AGLDLSRYRAVSIWCRRFSVNFGAAPLVATPS"
     gene            complement(869..1999)
                     /locus_tag="AC16JC_11060"
     CDS             complement(869..1999)
                     /locus_tag="AC16JC_11060"
                     /inference="COORDINATES: protein motif:HMM:NF021778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HIR5165277.1"
                     /translation="MTKHLTLLVLAAAALAGCERDRGISDPPRASVGAQAFADGGSGP
                     GAVYTLTNETTGNAVAVFARAADGTLTPMGTVATGGTGTGDGLGSQGAVVLSDDGRWL
                     FAVNAGSNDVSVFRVGPQGPSLASRTASGGTLPISLTVHGNVLYVLNAGGAGNITGFR
                     VRPDGELIAIAGATRPLSGSGVGPAEVAFSPDGRSLVVTEKATNLLDTYAVDQDGVAS
                     GPRSVASAGGTPFGFAFGLRNLLLVTEAADPGSASSYILDRGDDPRVVSGVVLTHQGA
                     PCWAVITNDGRLGFTGNGAGSVSAFAIGRDGSLSLLAAAAATITGGVNDIALSVNSRY
                     LYVLQVGATPAVHAFRVQDDGSLTSLGPVGDLPAGTRGLAAR"
     gene            complement(1996..2262)
                     /locus_tag="AC16JC_11065"
     CDS             complement(1996..2262)
                     /locus_tag="AC16JC_11065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165278.1"
                     /translation="MVFVSFMDEFPWQLDDDALAVPLDVGRWAVIRGGSPELRRGVAA
                     DLRALSRGPAGALTVRLARTLGAATPLEYLERTSTLTFAGGFTA"
     gene            2401..>2831
                     /locus_tag="AC16JC_11070"
     CDS             2401..>2831
                     /locus_tag="AC16JC_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013003.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HIR5165279.1"
                     /translation="MEIRHRETGEIIFDSTGSSLAGGRFRGARLHHAGLRGADLSGAD
                     LQGADLCIADLSGAALNGANLTGVDLSGATLSGAGLDGALLADAKLPYVNLANASLRH
                     ANLTRATLRYVNARGAAFTEAVRPRSDLSYGSCPGADFTGA"
BASE COUNT          438 a          968 c         1020 g          405 t
ORIGIN      
        1 gagagcagcg ccgccacccc cgtgaggtgc agaagtgtcg gggcgacggc gaacactgcg
       61 accgtggtga aatccagaat ccggtgcagg gccggtgcga tcagtcgcat gtgcgctcct
      121 tgggtcaacc aggggtaccg ccggaggtcg ccggccggtt cgccgcggca ggtgccacga
      181 cgagtccggc cggcgccagc ccttccggcg acaacgtctt cacgagggcg tggcgacgag
      241 cggggccgct ccgaagttca cactgaaccg ccggcaccag atcgacaccg cccggtacct
      301 gctcaagtcg aggccggcgg gcaggtcgta gttctgatcg ccgatgttgc ccttcaaggc
      361 gccgagatcc acgaacccgg cccgcttcgc ggcggcctca tcctgcacgt cggcggccgc
      421 cacgaggtag acacggacgt cggggccgtt ggaggtcgcg aactcggtca accgcagcac
      481 gagacgtccg tccgcaaggc gatagatcgt cgccgctccg gtggtctcgt gagcgttctt
      541 gtgaaaccgg ccggtcgtga ccagcaccgg ttctgccgcg gacaggcccg agtgggccat
      601 cgccggcgag tccgtcatgg cggaggccgg catcgtcatg cccggggtct ccggccttgc
      661 cgacatggag tgattctcgg cggggaaggg ctcgctgacg gtcttactga tgaccaacgc
      721 gtcgggccgg aacaggtacc agagcacggc gactcctacg actgccgcgc tgacgaagat
      781 cgatctacgg gttgtcaccg tacagctcct ttctcaagga ttcgtgaggg aggggcgcga
      841 acagccgcgc ccctccggtt gtggatcgct agcgggcggc gagtcccctg gtgccggcgg
      901 gaaggtcccc gaccggcccg agcgaggtga ggctcccgtc gtcctggaca cggaacgcgt
      961 ggactgccgg tgtggcgccg acctgcagca cgtacaggta ccggctgttg acgctcaggg
     1021 cgatatcgtt cacgccgccg gtgatcgtgg ccgcggcagc agccagcaga ctgagcgagc
     1081 catcgcgccc gatggcgaag gccgacaccg agccggcgcc attgccggtg aagccgaggc
     1141 gcccgtcgtt tgtgatgacc gcccagcatg gcgcgccctg gtgggtgagc acgacgccgc
     1201 tcacgacccg agggtcgtcg cccctgtcca ggatgtacga cgaggccgag cccggatccg
     1261 cggcttcggt caccaacagc agattgcgca ggccgaacgc gaacccgaac ggcgtcccgc
     1321 cggcggaggc taccgagcga ggtccgctgg ccacgccgtc ctggtcgacg gcgtatgtgt
     1381 ccagcaggtt cgtcgccttc tcggtgacca cgagtgaacg gccgtcggga ctgaacgcaa
     1441 cctcggcagg accgacaccc gagccggaga gcggacgcgt cgcgccggcg atcgcgatga
     1501 gctccccgtc gggacgcacc cgaaagcccg tgatgttccc ggcgcccccg gcgttcagca
     1561 cgtacagcac attgccgtgc acggtgaggc tgatcggcag tgtcccgccg gacgccgtgc
     1621 ggctcgcgag cgacggaccc tgcgggccca cgcggaacac cgagacatcg ttggagccgg
     1681 cgttgacggc gaatagccag cggccgtcgt cgctcaggac gacggcaccc tgcgagccca
     1741 gcccgtcgcc ggtgccggtg ccgcccgtcg ccacggtgcc catcggtgtc agggtgccgt
     1801 ccgccgcgcg cgcaaacacg gcgaccgcgt tgccggtcgt ctcgttggtc agcgtgtaga
     1861 ccgccccggg gccactgccg ccgtcagcga aggcttgcgc tccgactgag gctcgtggcg
     1921 ggtcactgat gccccggtcc cgctcgcacc cggcgagcgc cgcagctgcg aggaccagca
     1981 gcgtgaggtg ctttgtcatg cggtgaaacc tcctgcgaag gtgagagtac ttgtccgttc
     2041 cagatactcg agtggtgttg cggcacccag ggtgcgcgcc agccggaccg tgagggcgcc
     2101 ggcgggtccc cgtgagagcg cccggaggtc ggccgcgacg ccgcgccgga gctccggcga
     2161 accgccccgg atcacggccc accgccccac gtcgagcggc accgccagcg cgtcgtcgtc
     2221 gagttgccac gggaactcgt ccatgaagct cacgaagacc atgctccatc ccttatccag
     2281 ttggcggcca taatgcgggc gggtggggaa tggttcccgc ggatcccggc ctcgcgagga
     2341 ggctggcagt gcgcgtggtc gccggtacct tcccggggca gcgcccaccg gggggacgac
     2401 atggagatcc gacaccggga gaccggggag atcatcttcg attcgacggg cagctcgctg
     2461 gcgggcgggc gattccgcgg cgcgcggctg catcacgccg ggctgcgggg cgcggacctg
     2521 agcggggcgg acctgcaggg cgccgatctg tgcatcgccg atctatccgg cgccgcgctc
     2581 aacggtgcga acctcacggg cgtcgacttg agcggcgcga cgctcagcgg cgccggactc
     2641 gacggcgcct tgctggccga cgccaagctg ccgtacgtga acctggcgaa tgcgagtctg
     2701 cggcacgcga atttgacgcg cgcgacgctg cggtacgtga acgcgcgggg cgcagccttc
     2761 acggaggcgg tcaggccgcg gagtgacctg agctacggat cctgtccggg cgcggacttc
     2821 actggtgcgc a
//
LOCUS       DBNGZW010000471         4868 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1544506, whole genome shotgun sequence.
ACCESSION   DBNGZW010000471 DBNGZW010000000
VERSION     DBNGZW010000471.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1544506"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="AC16JC_11075"
     CDS             complement(<1..228)
                     /locus_tag="AC16JC_11075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018433724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidine dehydrogenase"
                     /protein_id="HIR5165280.1"
                     /translation="MTLPNQAPALPDEELSRNFAEIAPPLTRDAAALEANTCLFCYDA
                     PCTAACPTHIDLPAFIRKIASGNLRGAARVIR"
     gene            complement(225..1697)
                     /locus_tag="AC16JC_11080"
     CDS             complement(225..1697)
                     /locus_tag="AC16JC_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006970062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS1 family nucleobase:cation symporter-1"
                     /protein_id="HIR5165281.1"
                     /translation="MTEIGTHDPSLMNADLAPVGPERRTWNWWHIAALWIGMAICIPT
                     YTLASNLVDSGWTWQAAVGAVILGNIVVLVPIALNSHAGTRYGIPFPVFARASFGVLG
                     ANIPALLRGLVACGWFGIQTWIGGWAIYKLLEVLWPGIATLPRVLPGFVDLGTGEFLC
                     FLLFWGINVWIVLRGMDSIKFLETWGSPFLLLVGVALLVWAWVRAGGFGPMLENPAVG
                     RSGGHGGQAGLGGLFGAGLTSAVAFWGTMALSIPDFSRFARSQRDQMIGQAVGLPGTM
                     ALFAFIGAAVTSATAVIFGTRIADPVALLARIGGPVMIILSMVGLSIATLTTNIAANI
                     VAPANGFSNLAPDRISFKAGAVITAVIGIVMLPWRLYNDAAAYLFTWLIGYGALLGPI
                     AGIMIADYFGIRRGRLLVDDLYRRGGAYEYRGGFNWVAIAAFALGVLPNLPGFVAALS
                     GIPATPLFATLYNWAWFVGFLVAGILYLVGMKAQGARSRT"
     gene            complement(1838..3226)
                     /gene="hydA"
                     /locus_tag="AC16JC_11085"
     CDS             complement(1838..3226)
                     /gene="hydA"
                     /locus_tag="AC16JC_11085"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013014144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HIR5165282.1"
                     /translation="MPLLIKGGLVITATDEERADVYCRGETITRIAPGIDPRELSPDT
                     EVIDAAGKYVFPGFIDPHVHIYLPFMGTVAADDYASASRAALAGGTTTLIEMICPGPT
                     DEPWDAFELWRSKAQGLAAVDYTFHMSVVRFEEQVRDQFRKIVGAGVPSFKVFLAYKG
                     ALNLSDEHLFAAMKLAHELGVIVTAHCENADAIDALQKSLLAAGNTGPEWHGPSRPPA
                     VEADGVHHLCTFAELLGTHVYTVHTSCEPAVQAALAARYRGVNVWIESVVPHFVLDET
                     YAARADFEGAKYVMSPPLRDKANHEALWNGVKSRAISTVATDHAPFRFHDQKTMGREA
                     FTRIPNGIPSVQERVDLMHTHGVCAERFDLRTLVDACSTQAARLFGLYPRKGTIRVGS
                     DADLVVYDPAARGKFSAKGSYSNVDYSAYEGWDRIGRASVVTVRGAVQARDGQFVGTI
                     GRGQFLAREPTH"
     gene            complement(3333..4202)
                     /locus_tag="AC16JC_11090"
     CDS             complement(3333..4202)
                     /locus_tag="AC16JC_11090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002612486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR5165283.1"
                     /translation="MRSVVGGLIQMSNPLNDPTAPVTKVRDAMFEKHLPYIAEAGKRG
                     VQILCLQEVFNGPYFCPSQDSKWCDLAEPIPGPTIERMQQLAKQHAMVMIVPIYEREM
                     AGVYYNTAAVIDADGHYLGKYRKTHIPHTAGFWEKYFFKPGNLGYPVFKTRYATIGVY
                     ICYDRHFPEGARLLGLHGAEVVFNPSATVKGLSQYLWKLEQPAHAVANGFFMGCSNRV
                     GTEAPWNIGQFYGTSYFVDPRGNFLAVGSEDQDELVTAELNLDMIEDVRRTWQFYRDR
                     RPETYEDLVRQLP"
     gene            4334..4696
                     /locus_tag="AC16JC_11095"
     CDS             4334..4696
                     /locus_tag="AC16JC_11095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5165284.1"
                     /translation="MNKYQSLIAWKRAHALVLMALRVTDDAYHPRSRPLFDQIRRAVV
                     SVEANVVEGYALGSPRQFRKHLRIALGSAAEAECLVRTARELEYLPEDRAVELEDLLG
                     DTMRVLLGLLRSLTRTTT"
BASE COUNT          830 a         1623 c         1639 g          776 t
ORIGIN      
        1 gcggatgaca cgggcagcgc cgcgcaggtt gccgctcgcg atcttccgga tgaaggccgg
       61 caagtcgatg tgggtggggc aggcggccgt gcagggcgcg tcgtaacaga agaggcaggt
      121 attcgcctcg agcgccgccg cgtcgcgcgt gagcggcggc gcgatttcgg cgaagttgcg
      181 ggacagctcc tcgtccggca gcgccggagc ttggttgggg agcgtcacgt cctgctccgt
      241 gctccctgcg ctttcatccc gaccaggtag aggatcccgg ctaccaggaa ccccacgaac
      301 caggcccagt tgtacagcgt cgcgaacagt ggagtcgccg gtatcccaga gagcgcggcg
      361 acgaaccccg ggagattggg gagcacgccc agcgcgaacg ccgcgatcgc cacccagttg
      421 aagccgcccc ggtactcgta cgccccgccg cggcgatata gatcgtccac caggagacgc
      481 ccgcgccgga tgccgaagta gtcggcgatc atgatgccgg cgatcggtcc gagcagcgcc
      541 ccgtagccga tcagccaggt gaagagatag gcggcggcgt cgttatagag gcgccagggc
      601 agcatcacga tgccgatgac cgccgtgatc actgcgcccg ccttgaacga gatgcggtcg
      661 ggcgcgaggt tcgaaaagcc gttggcgggg gcgacgatgt tggccgcgat gttggtggtg
      721 agcgtcgcga tcgacagccc caccatcgac aggatgatca tcaccggccc gccgatgcgc
      781 gccagcagcg ccaccggatc ggcgatgcgc gtcccgaaga tcaccgccgt cgcgctcgtg
      841 accgccgcgc cgatgaacgc gaacagcgcc atcgtcccgg ggagccccac cgcctgcccg
      901 atcatctgat ctcgctggct gcgggcgaag cgggaaaaat cgggaatcga aagcgccatg
      961 gtgccccaaa acgccacggc cgaagtcagc cctgcgccga acagaccgcc cagaccggcc
     1021 tgaccgccgt gaccgcccga ccgcccgacc gccgggttct cgagcatggg accgaagccg
     1081 ccggcgcgca cccaggccca cacgagcagc gccacgccca cgagcagcag gaatgggctc
     1141 ccccaggttt ccaggaactt gatcgagtcc atgccgcgca gcacgatcca gacgttgatc
     1201 ccccagaaca gcaggaagca gaggaattcg cccgtgccca ggtccacgaa tcccgggagg
     1261 acgcgcggca gcgtcgcgat gccgggccac agcacctcga gcagcttgta gatcgcccac
     1321 ccgccgatcc aggtctggat gccgaaccag ccgcacgcca ccagcccgcg cagcaacgcc
     1381 gggatgttgg cgccgagtac gccgaaggag gcgcgcgcga acacgggaaa cgggatcccg
     1441 taccgggtcc cggcatgaga gttcagggcg atgggaacga gcaccacgat gttgcccagg
     1501 atgacggcgc cgaccgccgc ctgccacgtc cacccactgt ccacgagatt cgaggcgagc
     1561 gtgtaggtgg ggatgcagat cgccatcccg atccagaggg cggcgatgtg ccaccagttc
     1621 caggtgcgcc gctccggccc aaccggagcc aaatcggcgt tcatgagact ggggtcgtgc
     1681 gtcccgatct cggtcattgg gcctcacgtt gcgagggcgc tcgcggtgtc atcccgagcg
     1741 gagcgaggga tctcgcacct cgtccctgag attcctcact cgctgcgctc gttcggaatg
     1801 acaccggcgc gccgctcgtc gggtgcacca tccagcgtca gtgcgtcggc tcgcgcgcca
     1861 ggaactgccc gcgcccgatc gttcccacga actgaccgtc gcgcgcctgg acggcgccgc
     1921 gcaccgtcac gacgctggcg cgtccgatcc ggtcccaccc ctcgtaggcg ctgtaatcga
     1981 cgttcgagta gctgcccttc gcggagaact tcccgcgggc cgcggggtcg tagaccacga
     2041 ggtcggcgtc cgagccgacg cggatcgtcc ccttgcgggg gtagagcccg aacagacgcg
     2101 ccgcctgcgt gctgcaggcg tccaccaggg tacgcagatc gaaccgctcg gcgcacacgc
     2161 cgtgcgtgtg catcaggtcc acgcgctcct gcacgctggg aatgccgttg ggaatccgcg
     2221 tgaacgcctc ccggcccatg gtcttctggt cgtggaagcg gaacggggcg tgatcggtcg
     2281 cgacggtact gatggcgcgg gatttcacgc cgttccagag cgcctcgtgg ttcgccttgt
     2341 cgcgcagtgg cggggacata acgtacttcg ccccctcgaa atcggcccgg gcggcgtagg
     2401 tctcgtccag cacgaagtgc gggaccaccg actcgatcca gacgttcacg ccacggtagc
     2461 gcgccgccaa cgccgcctgc acggccggct cgcacgaggt atggaccgtg tagacgtgcg
     2521 tgcccagcag ctcggcgaac gtgcagaggt ggtggacgcc gtccgcctcg acggcgggcg
     2581 ggcgggaggg gccgtgccac tccggcccgg tgttcccggc cgctagcagc gacttctgta
     2641 gcgcgtcgat ggcgtcggcg ttctcgcagt gggccgtgac gatcacgccc aactcgtggg
     2701 ccagcttcat cgcggcgaac aggtgctcgt cggagaggtt gagcgcgccc ttgtaggcca
     2761 ggaacacctt gaacgacggc acgcccgcgc ccacgatctt gcggaactga tcgcgcacct
     2821 gttcctcgaa acgcaccacc gacatgtgaa acgtgtagtc caccgccgcg agcccctggg
     2881 ccttggagcg ccacagctcg aaggcgtccc agggctcgtc ggtcggcccg ggacagatca
     2941 tctcgatgag cgtggtggtg ccgccggcca gcgcggcccg gctcgccgag gcgtaatcgt
     3001 cggcggcgac ggtccccatg aaggggagat agatgtggac gtgcgggtcg atgaatcccg
     3061 ggaagacgta cttcccggcc gcgtcgatga cttccgtgtc gggcgaaagc tcgcggggat
     3121 cgatgccggg cgcgatgcgg gtaatggtct caccccggca atagacgtcg gcccgttcct
     3181 cgtcggtcgc cgtgatgacg aggccgcctt tgatcaggag gggcatgggg cgcacctgcg
     3241 gggagtgggg tgtggagagt ggtgcgcgac ggtgtgcacc gggttgacgc ctcgcgccgt
     3301 caggtaccac tccccactcc ccactccccg tttcatggca gctgcctcac cagatcctcg
     3361 tacgtctccg gccggcggtc gcggtagaac tgccacgtgc gccgcacgtc ctcgatcatg
     3421 tcgaggttca gctcggccgt caccagctcg tcctggtctt cgctgcccac ggccaggaag
     3481 ttgccgcggg gatcgacgaa gtagctcgtc ccgtagaact ggccgatgtt ccacggcgcc
     3541 tccgtgccca cccggttcga gcagcccatg aagaagccgt tggcgaccgc gtgcgccggc
     3601 tgctcgagct tccagaggta ctgggacagt cccttcaccg tggccgaggg gttgaacacg
     3661 acctcggcgc cgtgcaggcc gagcagccgc gccccttcgg ggaagtggcg gtcgtagcag
     3721 atgtagacgc cgatcgtggc gtagcgcgtc ttgaacacgg gatagccgag gttcccgggc
     3781 ttgaagaagt acttctccca gaaccccgcc gtgtggggga tgtgggtctt gcgatacttg
     3841 cccaggtagt gcccgtcggc gtcgatcacc gcggcggtgt tgtagtagac ccccgccatc
     3901 tcccgctcgt agatcggcac gatcatcacc atggcgtgtt gcttggcgag ctgctgcatc
     3961 cgctcgatcg tcggtccggg gatcggctcg gccaggtcgc accactttga atcctgcgag
     4021 ggacagaagt agggcccgtt gaacacttcc tgcaggcaga ggatctgcac gccccgcttg
     4081 ccggcttcgg cgatgtaggg aaggtgtttc tcgaacatcg cgtcgcgaac cttcgtgaca
     4141 ggcgccgtgg gatcgttcag cgggttggac atctggatga ggcccccgac gacgctacgc
     4201 atcggcgcct cgggcgaagg cggttgtgcg gggagtgggg agtgggcagt ggtaccccac
     4261 ggtgcaggtg aacgatacgt gtcgcgagaa aaagatcacg agactcgtgt ccagtctgag
     4321 tagtgtgcga ttcatgaata agtaccagtc cctaatagcg tggaaacggg cgcacgctct
     4381 ggtgctgatg gcgctccgcg tcacggatga tgcttaccat ccccgctctc gtccactgtt
     4441 cgaccagatt cgtcgcgcgg tggtttccgt cgaagcgaac gtggtcgagg ggtacgccct
     4501 cggctccccg aggcaattcc gaaaacacct gcgaatcgcc ttgggatctg cggctgaggc
     4561 ggaatgcctc gtccgcaccg ccagggagct ggagtatctg cccgaggata gggccgttga
     4621 gctggaagac ctcctcggcg acacgatgcg agtgctcctg ggcttgctcc gctccctcac
     4681 gcgaaccacg acgtgaatgt tcacccgtcc aggtcccact ccccactccc cgtctctcag
     4741 aaccacctcg agatgacgac cttcttgtcg gtgaagaact gcacgctgtc ctgcccgtgc
     4801 gccttgagat cgccgaagaa cgatcccttc gtgccgccga aggggaagaa cgccatcggg
     4861 gccgccac
//
LOCUS       DBNGZW010000472         2830 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_385603, whole genome shotgun sequence.
ACCESSION   DBNGZW010000472 DBNGZW010000000
VERSION     DBNGZW010000472.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_385603"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="AC16JC_11100"
     CDS             complement(<1..442)
                     /locus_tag="AC16JC_11100"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5165285.1"
                     /translation="MTTTTTTTTPVLTRLLAEGYGPGAWHGADMKAALSDVSPRAAFW
                     RPGPGRHSIAEIALHHAFYVRSVIGRLTGQEPAPFVLEGEDWFALPDVGELDWSAIRT
                     VVEELQQRLAAVAAEIEAGRVRSPLSEPERFNLALGITCHAIYHA"
     gene            511..1416
                     /locus_tag="AC16JC_11105"
     CDS             511..1416
                     /locus_tag="AC16JC_11105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020722139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5165286.1"
                     /translation="MVSARRPPVLGKIAAELERAFARRREAGSPGGTAGVRLAAGPGW
                     SVHDVLCTYGPGDRPFEERHEAVAIAVVIGGTFRYRTPSGHEVMTPGSLLLGNAGQPF
                     ECGHEHGAGDRCVSFRYEPGCFEQIRADAAPAHGHRFRVLRLPPLRDTAPLVARAVIG
                     VARGAAAWEELALETAAAALGLAAGVPGSRRPLPARAVARVTQSVRAIERDPGSRVSL
                     SRLAAEAGLSPYHYLRTFTLVTGVTPHQFIVRARLREAAARLARERTRVIDVALESGF
                     NDVSNFNRAFRAEFGIAPRAYRQRA"
     gene            complement(1425..1622)
                     /locus_tag="AC16JC_11110"
     CDS             complement(1425..1622)
                     /locus_tag="AC16JC_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165287.1"
                     /translation="MLVIEDIAYEGFRRRVVLPGVSAVEASRWRRKLYPDTPTTRIGC
                     SESWSSGAVVGGAFIEQAAGC"
     gene            complement(1768..2355)
                     /locus_tag="AC16JC_11115"
     CDS             complement(1768..2355)
                     /locus_tag="AC16JC_11115"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR5165288.1"
                     /translation="MTRLMRFAALAMVATALAVPAGAQQRSLIFSVYGGGADHLADLR
                     SSPPVWFMPGYDLGASVGLELSRHVAVHGDFTYTRNPTRGSSSFAGADVNRFYYGVHV
                     EGRYPLSIGLDPFVFGGVGAVSVDQLGLDTFKPFTRPALMYGGGLFYPIPRSRVGVFG
                     EVKGLTYRWNTAGFQRTMFDVTYSVGASYRLPVNF"
     gene            2483..>2830
                     /locus_tag="AC16JC_11120"
     CDS             2483..>2830
                     /locus_tag="AC16JC_11120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HIR5165289.1"
                     /translation="MTASWHTLLDVALRTTVVYLVLLVGIRLTGQRLLGQLSAFDLVL
                     LLIIANAVQNAMVGPDTSLAGGLVAAGVLLFWHQVINRVRRSSPRFGKLIAGEGVMLI
                     KDGKILEQHVQHAG"
BASE COUNT          440 a          990 c          973 g          427 t
ORIGIN      
        1 ccgcgtggta gatcgcgtgg caggtgatcc ccaacgcgag attgaaccgc tcgggctcgg
       61 acagcggaga gcggacccgt ccggcctcga tttccgcggc cacggcggcg agccgctgct
      121 gcagctcctc cacgaccgtc ctgatcgcag accagtcgag ctctcccacg tccgggagcg
      181 cgaaccaatc ctcgccctcg agcacgaacg gcgcgggctc ctgcccggtg agccggccga
      241 tcacggagcg gacatagaat gcatggtgca gcgcgatctc ggcgatgctg tggcggccgg
      301 gaccggggcg ccagaaggcc gcgcgcggcg agacgtcgct cagggccgct ttcatatcgg
      361 cgccatgcca ggcgcccggc ccgtatccct cggccagcag cctggtcagt acgggagtcg
      421 tggtggtcgt tgtggtagtc atcgggtcct cgcggaatcg tggttctccg ggggattgcg
      481 gcacgacccc cgggctttgg atgtttgagc atggtatccg cccggcgccc gcccgtcttg
      541 ggaaaaattg cggccgagct ggagcgggcg ttcgcccggc gccgggaagc cggatcgccc
      601 ggcggcaccg ccggcgtccg gctcgccgcc ggccccggct ggtcggtcca cgacgtcctc
      661 tgcacctacg gtcccggcga ccggccgttc gaggaacgtc acgaggctgt cgccatcgcc
      721 gtcgtgatcg gcgggacgtt ccgttaccgc acgccgtccg ggcacgaggt gatgacgccc
      781 ggatctctgc tgcttggcaa cgccggccag ccgttcgagt gcggtcacga gcacggcgcg
      841 ggcgatcgct gcgtgtcctt ccgctatgag cccgggtgct tcgagcagat tcgcgccgat
      901 gccgctccgg cacacggaca ccgcttccgt gtcttgcgtc tgccgccgtt gcgggatacg
      961 gcgcccctgg tggcccgggc ggtgatcggc gtcgcgcgcg gcgcggcggc ctgggaagag
     1021 ttggcgctgg aaacggcggc cgccgcgctc ggcctcgcag ccggagtgcc cgggtcgcga
     1081 cgcccgctgc ccgcgcgggc cgttgcgcgt gtgacgcaga gcgtgcgcgc gatcgaacgg
     1141 gatcccgggt cgcgggtgag cctgagccgg ctcgccgcgg aggccgggct cagcccctac
     1201 cactatctgc ggacgttcac actggtgacg ggggtgacgc cgcaccagtt catcgttcgc
     1261 gcccgcctga gagaagcggc cgcacgattg gcgcgggagc gcaccagagt gatcgacgtc
     1321 gccctcgagt cgggcttcaa cgacgtgtcg aatttcaacc gggcgttccg ggcggaattc
     1381 ggcatcgcgc cgcgggccta tcggcagcgc gcctgatgtg ccgatcagca gccggccgct
     1441 tgctcgatga acgccccgcc aaccaccgct ccggaactcc acgactccga gcagccgatc
     1501 ctcgtggtcg gcgtgtccgg atagagcttc cttctccacc gggaggcttc gaccgcgcta
     1561 acgccgggca gaaccacgcg cctcctgaac ccttcgtatg cgatgtcctc gatgacgagc
     1621 acaccggatg gcagcttgag gacgagggcg gacgccgcac gatcctcccg ccgagcacgg
     1681 gactctgcaa caaggccggt cggcctgcca taacgagcga cggccggggg gccccggccg
     1741 tcgcagcaga acccggcagg tgtccgccta gaagttgacc ggcaggcggt agctcgcccc
     1801 gaccgagtac gtcacgtcga acatcgtccg ctggaagccg gcggtgttcc agcggtaggt
     1861 cagccccttc acctccccga acaccccgac ccggctgcgc ggaatgggat agaacagccc
     1921 acccccatac atcagcgccg gacgagtgaa cggcttgaag gtatccaggc cgagttgatc
     1981 caccgacacc gcccccaccc cgccgaacac gaacgggtcc aacccgatcg acagcggata
     2041 gcgcccttcg acatgcaccc catagtagaa gcggttcacg tccgctccgg cgaaggacga
     2101 cgacccccgc gtggggttcc gggtgtaggt gaagtcgccg tgcacggcga cgtgccggct
     2161 gagctccagc cccacgctgg cgccgaggtc gtagccgggc atgaaccaca ccggcgggct
     2221 gctgcgcagg tccgccagat gatcggcccc gccgccataa acactgaaga tcagagaccg
     2281 ctgctgggcc cccgccggga cggcgagcgc agtcgccacc atggccagag ccgcgaaccg
     2341 catcaagcgc gtcatgggtt tcctccttgc cgggcagtat gaagagacac cttcaggcta
     2401 gggggcggat tctgaagacg ggatgagggg acgatgatga ctgcttgacg gttccgcgcc
     2461 gggagtgatg attgcaccgt ccatgacggc ctcctggcac accctgctcg acgtcgcact
     2521 gcggacgacg gtcgtctacc tcgtgctgct ggtggggatc cgcctcaccg gccagcgcct
     2581 cctcggccag ctgtcggcgt tcgacctggt gctgctgctg atcatcgcga acgccgtgca
     2641 gaacgccatg gtgggcccgg acacgagcct cgcgggcggc ctcgtggctg ccggcgtcct
     2701 gctcttctgg caccaggtga tcaatcgggt gcggcgctcg agcccacgct tcgggaagct
     2761 catcgcgggc gagggcgtca tgctgatcaa ggacggcaag atcctcgagc aacacgtgca
     2821 gcacgccggc
//
LOCUS       DBNGZW010000473         3281 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1054426, whole genome shotgun sequence.
ACCESSION   DBNGZW010000473 DBNGZW010000000
VERSION     DBNGZW010000473.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3281
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1054426"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..880)
                     /locus_tag="AC16JC_11125"
     CDS             complement(<1..880)
                     /locus_tag="AC16JC_11125"
                     /inference="COORDINATES: protein motif:HMM:NF012879.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5165290.1"
                     /translation="MAWAVMLSLAAPALTAQEPSPGERLPVVKHVLPNGMTFLLLRRG
                     GAPTVSFVTHFRAGGVDEWTGISGTAHLFEHMLFKGTHSIGTTDDSAEQALFPAIDAV
                     ADSFTAEFRKGAGADSTRQEALRGRLKQLEDSARHFVVSNELDRILTENGAQFLNAST
                     ANDLTNYFFQLPANRAKLWFVLESDRIRNPVLREFYSEREVVMEERRLRVETQPAGLL
                     REEFLQAAFRAHPYGRPVVGVASEIQAVTRRAAQEYFKRFYGPNNAVVAIVGDIDVDS
                     MTAWADAYFADVPAGEP"
     gene            1027..2340
                     /locus_tag="AC16JC_11130"
     CDS             1027..2340
                     /locus_tag="AC16JC_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR5165291.1"
                     /translation="MALVWAVLPLACGEPTRAPEPFTIRLVANDVWSGGELQLVAAAF
                     VPPASLPAVLLDGDSLPVRRIDDTTIAARLPVATGTFSLRVVRSRDMALLGDVILHGF
                     RDEVVGPFLSGRVYVLQPGLPLIIGAGNSGAAVVDLRFNTIAQTIPDSVHSPDCVWSV
                     GPSYQPGWFVFEGRDAAGVCGPPSLWSIAPSLQLVDALPGWSWPVSWYVMAVPGPRRW
                     FLDWNNHGYFLVCDTGCVTEFFGDPSGPDMVIISPRGDRLLWTPQCCTVAVFDAATMD
                     TAFAVPMSSDPLYESQSYGAAFSAAGDTLFVSVVGPGPHLVAVRASDGLMLTDVRLDS
                     LSLGLPVSFIELGPIALDPQHPWLYMSVVLRLDSLIVPGVIVFDRTTLSPLGVLPAFG
                     ASGRYHMPTVVPSPLEHLVYLVATDISGGGIHGSRARILRFETVP"
     gene            complement(2347..3174)
                     /gene="mutM"
                     /locus_tag="AC16JC_11135"
     CDS             complement(2347..3174)
                     /gene="mutM"
                     /locus_tag="AC16JC_11135"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF002211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIR5165292.1"
                     /translation="MPELPEVETIVRDVAPRLVGRRIRSAKLTKTDVLRGVSRPRLVR
                     TLTGNTVRAASRRAKHAVFLLQSGHRMIVQPRMTGSLIVYDRPLTPDEARYAVLTCSL
                     AGGGLFVYRDVRRLGTVWLLDERGWKAYTARLGPEPLEPGFTPAVLARALAGTRAAVK
                     KDIMDQRRLAGVGNIYANEALFDAGIDPSRPANRLTAAEVERLHGAIVEILQRALRAS
                     GTTVRDYRTGTGGTGSFQLELNVYDRGGEPCVRCGRRLVTTHAIDLRQTTMCAFCQR"
BASE COUNT          456 a         1105 c         1136 g          584 t
ORIGIN      
        1 gcggctcgcc cgccgggacg tcggcgaagt aggcgtccgc ccacgcggtc atcgagtcca
       61 cgtcgatgtc gcccacgatc gcgaccacgg cgttattcgg tccgtagaac cgcttgaagt
      121 actcctgcgc ggctcgacgc gtgaccgcct ggatctcgct cgccacaccg accaccggcc
      181 gtccgtaggg gtgggcgcgg aacgccgcct ggaggaactc ctcccgcaac aggccggccg
      241 gctgcgtctc cacgcgcagc cgccgctcct ccatcaccac ctcgcgctcc gagtagaact
      301 cgcgcagcac cgggttcctg atgcggtcgc tctcgagcac gaaccacagc ttggcgcggt
      361 tggcggggag ctggaagaag tagttggtca ggtcgttcgc ggtggaggcg ttgaggaact
      421 gcgcgccgtt ttcggtgagg atgcggtcca gctcgttgga gaccacgaaa tggcgcgccg
      481 aatcctcgag ctgcttgaga cggccgcgca gggcctcctg gcgggtgctg tccgcgccgg
      541 cgcccttgcg gaactcggcg gtgaacgagt cggccaccgc gtcgatcgcg gggaacagcg
      601 cctgctcggc cgagtcgtcg gtcgtgccga tggaatgggt gcccttgaac agcatgtgct
      661 cgaacagatg agcggtgccg gagatcccgg tccactcgtc cacgccgccg gcgcggaagt
      721 gcgtgacgaa ggagacggtc ggcgcgccgc cgcgacgcaa caacaggaag gtcatgccgt
      781 tggggaggac gtgcttgacg accggcagcc gctcgcctgg ggaaggctcc tgggccgtga
      841 gcgcgggagc ggcgagactg agcatgacgg cccaggccac aagacgcatc gacgacctcg
      901 gtgtcggggg cgtgacgtgg aaggtagtgt cgtcggggag cgcgtcgcca gggagttgtg
      961 gtcccctggg ttacgccata ttctgaagct cgcggagagt ggtgcatgct cggtcgttcg
     1021 tgcccgatgg ctctcgtatg ggcggtcctc ccgctcgcct gtggcgagcc gacgcgggcc
     1081 cccgagccct tcactatccg gctggtcgcc aacgacgtct ggagtggcgg cgagcttcag
     1141 ctcgtcgctg cggcgttcgt cccgcccgcc tcgctaccgg ccgtgctgct cgacggcgac
     1201 tccctgcccg tgcgacggat cgacgacacg accatcgcgg cgcgtctccc ggtggctacc
     1261 gggactttct cgctccgcgt cgtgcgctcg cgcgacatgg cgttgctcgg agacgtcatc
     1321 ttgcatggct ttcgcgatga ggtcgtcggg cccttcttgt cgggacgcgt gtatgtgttg
     1381 cagccgggcc tcccactcat catcggggcg gggaactcag gagccgcggt ggtggacctg
     1441 cggttcaaca ccatcgccca gacgatcccg gactcggtgc actctcccga ctgtgtctgg
     1501 agtgtggggc cctcctatca gcctggatgg ttcgttttcg aggggaggga cgccgctggt
     1561 gtgtgtggcc cccccagttt gtggagcatc gccccctcgc tgcagctggt ggacgccctc
     1621 cccggctgga gctggcccgt ttcctggtac gtcatggcgg tcccggggcc gaggcgctgg
     1681 ttcttggatt ggaacaatca cgggtacttc ttggtgtgcg atacaggatg cgtcacggag
     1741 ttcttcggag atccgagcgg ccccgatatg gtgatcatct caccccgcgg cgaccggctc
     1801 ctgtggaccc ctcagtgctg cacggtggcg gtgttcgatg ccgctacgat ggacacggcg
     1861 ttcgcggttc ccatgtcctc ggacccactc tacgagtcgc aatcctacgg cgcggcgttt
     1921 tccgcggccg gggatacgct ctttgtctcg gtcgtcgggc cgggtccaca tctcgttgcc
     1981 gtgcgtgcca gcgacggctt gatgttaacg gacgtacggc tcgactcgct ctcgctcggt
     2041 ctgcccgtct cgttcatcga actgggaccg atcgcgctcg acccgcagca cccctggctc
     2101 tacatgtcgg ttgtgcttcg cctggattcg ctcatcgttc ccggggtgat tgtcttcgac
     2161 cgtacgacgc tctcgccgct cggcgtcctg ccggcattcg gtgcgagcgg ccgataccat
     2221 atgcccaccg tggttccgag cccactcgag cacctggtct acctcgtggc gacggacatc
     2281 agcggcggag ggatccacgg tagtcgtgca aggatcttgc ggttcgaaac ggttccatag
     2341 tgtcccctaa cgttggcaga acgcgcacat cgtcgtctgg cggagatcga tcgcgtgcgt
     2401 ggtcacgagc cgccggccgc agcggacgca cggctcgccg ccgcggtcgt agacgttcag
     2461 ctccagctgg aagctgcccg ttcccccggt gccggtgcgg taatcccgca cggtggtccc
     2521 cgacgcgcgc agggcgcgct gcagaatctc gacgatggcg ccgtgcagtc tctcgacctc
     2581 cgcggccgtg agccggttgg ccgggcggga agggtcgatg cctgcatcga acagcgcctc
     2641 gttggcgtag atgttgccga cgccggcgag gcgacgctga tccatgatgt ccttcttcac
     2701 cgcggcgcgt gtgccggcga gcgcgcgggc cagcacggcg ggcgtgaagc cgggctcgag
     2761 cggctcgggc ccgagccggg cggtgtacgc cttccagccg cgctcgtcca gcagccagac
     2821 ggtgcccaga cggcgcacat cgcgatacac gaacaggccg cccccggcca gggagcaggt
     2881 cagcaccgcg taccgcgctt cgtcgggggt gagcggccgg tcgtagacga tcagcgaccc
     2941 cgtcatgcgg ggctgcacga tcatgcggtg cccgctctgc agcaggaaga cggcatgttt
     3001 cgctcgacgc gaagccgcgc gcaccgtgtt gccggtgagc gtgcgcacga gtctcggacg
     3061 ggacacgccg cgcagcacgt cggtcttcgt gagcttggcg gaacggatgc gacgtcccac
     3121 gagccgcggc gccacgtcgc ggacgatcgt ttcaacttca ggcagctccg gcacgcccca
     3181 ccccgcggct ccgatcgatc tcccgccgtg cgatgtcgcg gcgccaggtc ttgccgtcga
     3241 acgcgatccg ctcgcacgcc agccggctgg ccgccgcggc c
//
LOCUS       DBNGZW010000474         3422 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_862043, whole genome shotgun sequence.
ACCESSION   DBNGZW010000474 DBNGZW010000000
VERSION     DBNGZW010000474.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3422)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3422)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3422
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_862043"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..886)
                     /locus_tag="AC16JC_11140"
     rRNA            complement(<1..886)
                     /locus_tag="AC16JC_11140"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1196..>3422
                     /locus_tag="AC16JC_11145"
     CDS             1196..>3422
                     /locus_tag="AC16JC_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5165293.1"
                     /translation="MTSPNACARCGAPLAPGARFCSRCGHDVSGVQGGLATERLAVPP
                     DLDDPQAGLLDELRQATLGEFEIAGELGRGGMATVFLAHDISLDRKVAIKVMSPALLA
                     SGKGMADRFKREARTAAALSHPHIIPIYGVRQSGRLLYFIMKYVQGRGLDAIVKEHGP
                     LPIKMVQTILSQTGSALDYAHRHGIIHRDIKPANIMLDVEGWAVVTDFGIAKVTESQG
                     LTMTGATIGTPTYMSPEQCAAKEITGATDQYSLGIAAYEMLTGRVPFDADSVMSLMWQ
                     HFHEPPPPILDRRPDCPPHIAQAIERMLAKKPADRWASLEEAVEAIGTPPHNDPVRQQ
                     MKALARGGSLADAAQWATPVSPVPPYAKPATPPPSPVMTAAPTTPLPSTPTTPIPAAP
                     TTPIPATAAAPVSPPEAPPSLPVTPVEPITPEQPPEVTEGPTAVFAKRTRPPAPPPQP
                     PPARKAGRRPSGGVPAPGRASRAEPAPTPPPPPYAAPRRTNTMQLVGAAAVLVVLGVA
                     AWTIFARNKTPAPPAAEPQTPPPAEVASVSVTPSPTAIAVGRTAQLTVVLKDGRGNVL
                     EGRPVGWSSSDSTVARVSPAGLVSAVAPGSVSVLATSEGRTGTASVTVTTTAAPVARV
                     DIPPATAIALGDTTALTAQPKDDRGNALSGHVLTWSSSDRGVVAVSPSGTLIALRLGT
                     AVITVESEGKSATTRVTVTPPAVAIVEITPPTASLQPGAHAQLSVRARDSHDRPISDR
                     RV"
BASE COUNT          549 a         1350 c         1008 g          515 t
ORIGIN      
        1 cggggcactt actgcgttag cgacggcacc gggagggtcg ctccccccga cgcctagtgc
       61 ccatcgttta cggcgtggac taccagggta tctaatcctg tttgctcccc acgctgtcgc
      121 gcctcagtgt cagcaacggc ccagcagaac gccttcgcca ctggtattct tcccgatatc
      181 tacgcattcc accgctacac cgggaattcc atctgcccct accgtgctca agtccgacag
      241 tcttgccggc agccccggag ttgagccccg ggatttcacc agcaacttac cgaaccacct
      301 acgcgccctt tacgcccagt gattccggac aacgctcgca ccctccgtat taccgcggct
      361 gctggcacgg agttagccgg tgcttccttt ggaggtaccg tcactcgaga tcgcctattc
      421 gacgtcccgc attcgttccc cctgacagtg gtttacaatc cgaagaccgt catcccacac
      481 gcggcgtcgc tgcgtcagcc ttgcggccat tgcgcaatat tccccactgc tgcctcccgt
      541 aggagtctgg gccgtgtctc agtcccaatg tggctgatca tcctctcaga ccagctaccc
      601 gtcgtcgcct tggtgggccg ttaccccgcc aactagctga taggccgcga ggccctccgt
      661 cggcgcaacc ttgcggctgc tttcctcgcc cagggatgcc cccgaacgag cgtatgcggt
      721 attacccggc cgttgggccg gctatccccc gccaccggac aagttcctca cgtgttacgc
      781 acccgtctgc cactgaacct ggagttgccc ccaggtcccg tacgacttgc atgtgttagg
      841 cacgccgcca gcgttcgtcc tgagccagga tcaaactctc caatagagaa ttcgaacagc
      901 tcaaatcgaa cgactgcgtt actgcttgtc gtccgaatca cattcagagt caaacccctg
      961 acgacacgcg tctgaccgcg tatcgctcat atcggggccg cactccacac gctcgaccat
     1021 caattctcaa agagcgcacc acgaaaaaac tcgtggtgag actcacattc tacccaccca
     1081 cccccctcgc gtcaatgggg ccccccactt ggagatacga cgcccgaccc acaccgtccc
     1141 gcctttgccg ccccagctcc cgcgggctaa attcgcccac gcgtccgccc acaccatgac
     1201 ctcgcccaac gcctgcgccc gctgcggcgc tccgctcgcc cccggcgccc gcttctgctc
     1261 gcgctgcgga cacgacgtgt ccggcgtcca gggcggcctc gccaccgagc gcctggccgt
     1321 gcccccggat ctcgacgacc cgcaggccgg actcctcgac gagctgcgcc aggccacgct
     1381 gggcgagttc gagatcgccg gcgaactggg ccgcggcgga atggcgaccg tcttcctcgc
     1441 ccacgacatc tcgctcgatc gcaaggtcgc gatcaaggtc atgtccccgg cgctgctcgc
     1501 cagcggtaag ggcatggccg atcgtttcaa gcgggaggcc cggaccgccg ccgccctctc
     1561 gcacccccac atcatcccga tctacggggt gcgccagtcg gggcgactcc tctacttcat
     1621 catgaagtac gtgcagggac gcgggctgga cgcgatcgtc aaggagcatg gccctctgcc
     1681 catcaagatg gtgcagacga ttctgtcgca gaccggcagc gcgctcgact acgctcaccg
     1741 ccacggcatc atccatcgcg acatcaagcc cgcgaacatc atgctcgacg tggaggggtg
     1801 ggccgtcgtg accgacttcg gcatcgccaa ggtcaccgag tcgcaagggc tcaccatgac
     1861 cggcgccacc atcgggacgc cgacctacat gagccccgag caatgcgcgg cgaaggagat
     1921 caccggcgcc accgatcagt actccctcgg catcgcggct tacgaaatgc tcaccggccg
     1981 cgtcccgttc gatgccgatt cggtgatgtc gctcatgtgg cagcacttcc acgagccgcc
     2041 cccgccgatt ctcgatcgcc gtccggactg tccgccccac atcgcgcagg cgatcgagcg
     2101 catgctcgcc aagaaaccgg cggaccgctg ggcgtcgctc gaggaagccg tcgaagccat
     2161 cggcacgccg ccgcacaatg atcccgtccg ccagcagatg aaagcgctgg cgcgcggggg
     2221 ctcgctcgcc gacgccgcgc aatgggccac gcccgtgagc cccgtgccgc cctacgccaa
     2281 gccggcgacg ccgcctccct cgcccgtcat gacggcggcc cccacgacgc cgctgccgtc
     2341 cacgccgacc accccgatcc cggcggcccc gacgacgccg atccccgcca ccgcggccgc
     2401 gccggtttct cccccggaag cgccaccgtc gctgccggtg acgccggtcg agccgatcac
     2461 gcccgagcag ccgcccgagg tcaccgaggg ccccacggcg gtcttcgcca aacggacccg
     2521 ccctccggct ccgccgccgc agccgccgcc ggctcgcaaa gcgggtcggc gaccctcggg
     2581 gggagtcccc gcacccggac gcgcctcgcg tgccgagccc gcgcccacgc ccccgccccc
     2641 gccctacgcg gcaccgcggc gcacgaacac aatgcagctc gtcggcgccg ccgccgtgct
     2701 cgtggtcctc ggcgtcgccg cctggacgat cttcgcccgc aacaagaccc ccgcgccccc
     2761 ggccgccgaa ccccagacgc ccccgccggc agaggtggcg tccgtctcgg tgacgccctc
     2821 ccccaccgcg atcgcggtgg gacggaccgc gcagctgacg gtggtgctga aagacgggcg
     2881 cggcaacgtc ctcgagggac gaccggtcgg ctggagctcg agcgacagca cggtggcgcg
     2941 cgtgtcgccc gccgggctgg tgagcgccgt cgctcccggt tcggtcagcg tgctggccac
     3001 gagcgagggc cgtaccggga ccgcgagcgt cacggtgacg acgacggccg ccccggtcgc
     3061 gcgcgtcgac atccccccgg ccaccgcgat cgccctgggg gacacgaccg cactcacggc
     3121 ccaaccgaag gacgaccgcg gcaacgcgct ctcgggccac gtgttgacct ggagctcgag
     3181 cgaccgtggc gtggtcgcgg tgtcgcccag cggcacgttg atcgccctgc ggctcggcac
     3241 cgcggtcatc accgtcgaat ccgagggcaa gtcggcgacg acgcgcgtga ccgtcacacc
     3301 gcccgccgtc gcgatcgtgg agatcactcc gcccacggcc tcgctgcagc ccggcgccca
     3361 cgcgcagctt tccgtgcgcg cgcgggattc gcacgaccgg ccgatcagcg accgcagggt
     3421 ga
//
LOCUS       DBNGZW010000475         1637 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_181199, whole genome shotgun sequence.
ACCESSION   DBNGZW010000475 DBNGZW010000000
VERSION     DBNGZW010000475.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1637)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1637)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1637
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_181199"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..792)
                     /locus_tag="AC16JC_11150"
     CDS             complement(<1..792)
                     /locus_tag="AC16JC_11150"
                     /inference="COORDINATES: protein motif:HMM:NF014428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5165294.1"
                     /translation="MKRRRWQMLCVLGAAAGCGGGGSSGPNTNPSQVVASAGENQVGV
                     AGQQLGAALEVTVKDAAGSPVANVTVTWAVASGGGSVTPASNTTGADGKATATRTLGP
                     GAGAQTATATVSGVTPATFHHIAQIQGATQIAANVGLIRSDSVLSTAPFSVVVRDQNS
                     QAVAGVTVTWSLSGGGGNLSQPTSVTDASGIASVTLTLDQVAAPRSLQAAVTGLQGSP
                     VQFAENGVAGNATQMALSGGNFQAGPVGGDLATQLGVLVKDAHGNV"
     gene            1066..>1637
                     /locus_tag="AC16JC_11155"
     CDS             1066..>1637
                     /locus_tag="AC16JC_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165295.1"
                     /translation="MDVDLTLKRWGVPVRNGSRYVGCRLSYDGPWVIAPVRMRPAAGP
                     PSGKERRSAERLTLELAGLCLGLSDRRDAPTIGQPPAIAIDPLKDLLTLPAVVAHEAK
                     NPLSGARAGLQLTMESLGRMEDLPVRRRLELLEEMGEVLEALNRALDFLQAVQDRARG
                     AIPGRERFDAVRVARSVVALEGRVLRQVGV"
BASE COUNT          225 a          561 c          590 g          261 t
ORIGIN      
        1 cacgttgccg tgagcatcct tcaccagcac gccgagctgg gtcgcgaggt cgccgcctac
       61 gggccccgcc tggaagttgc cgccgctcag ggccatctgt gtcgcattcc ccgccacgcc
      121 gttctcggcg aactgcaccg gagacccctg gaggccggtc actgccgcct gcagcgaacg
      181 gggcgcggcg acttggtcga gcgtcagggt gacgctggcg atgccgctcg cgtccgtgac
      241 cgaggtgggc tggctcagat tcccgccccc gccgctgagc gaccaggtga ccgtcacgcc
      301 ggcgaccgcc tggctgttct ggtcccgaac cactactgag aagggggctg tgctgagcac
      361 gctatccgaa cgtatcaggc cgacattcgc cgcgatctgg gtggcgccct ggatctgggc
      421 aatatgatgg aaagtggccg gggtaacccc cgacacggtg gcggtggccg tctgcgcacc
      481 ggcgcccggc ccgagcgtgc gcgtggcggt cgccttgccg tccgccccgg tggtgttgct
      541 ggcgggcgtc accgatccgc cgccgctcgc cacggcccag gtgacggtga cgttggccac
      601 ggggctcccc gcggcgtcct tgaccgtgac ctccagggcc gctcccagct gctggcctgc
      661 caccccgacc tggttttcac ccgccgaggc gacgacctga gatgggttgg tgttgggacc
      721 cgagctgccg ccgccgccgc agcccgccgc cgccccgagg acacagagca tttgccaacg
      781 acgacgcttc acgaggcacc tccgggtttg tgaagtaacc gagattgtac catttgcggc
      841 ctattgttgc tactttaggt gcgcctttca cagccatggc ggattcacac cagggggtgc
      901 gcccgggtgg cgacggagac atttcgaggc agtgccgggg agattctccg gcaagcgagc
      961 gtagcactcg acggcaagcc ggtgacgctc tgggaggtcg ccgacgggcc atcgttgata
     1021 ccccgagcgt caagcgatcc cagcccgcgt caccatttca ccaacatgga cgtcgacctc
     1081 accctcaagc ggtggggcgt ccccgtgcgc aacggcagcc gctacgtcgg gtgccggttg
     1141 tcctacgacg ggccctgggt gatcgcgcct gtgcgcatgc ggcccgcggc gggaccgccc
     1201 tcggggaagg agcggcgcag cgccgagcgc ctcacgctgg agctcgccgg cctgtgcctg
     1261 ggtctctccg atcgccggga tgcgcccacc atcgggcagc cgccggccat cgcgatcgat
     1321 ccgctgaagg acctgctcac cctgccggcg gtggtcgcgc acgaggcgaa gaatcccctc
     1381 tccggcgcgc gggcggggtt gcagctgacc atggagtcgc tgggacgcat ggaggatctg
     1441 ccggtgcggc gccggctcga gttgctggag gagatggggg aagtgctgga agcgctcaac
     1501 cgggcgctcg acttcctgca ggcggtgcag gatcgcgccc gcggcgcgat cccggggcgc
     1561 gagcggttcg atgcggtgcg tgtggcccgc tcggtcgtgg cgctggaggg acgggtgctg
     1621 cgccaggtcg gggtgga
//
LOCUS       DBNGZW010000476         1476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_523355, whole genome shotgun sequence.
ACCESSION   DBNGZW010000476 DBNGZW010000000
VERSION     DBNGZW010000476.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_523355"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16JC_11160"
     CDS             complement(<1..230)
                     /locus_tag="AC16JC_11160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165296.1"
                     /translation="MKHLFAASLALLGSVAAACYQDDTLHPAAISPTQVLITDDPFPF
                     DTVASVNIYVSRIEATTDLDTTGTWVTIATPN"
     gene            339..878
                     /locus_tag="AC16JC_11165"
     CDS             339..878
                     /locus_tag="AC16JC_11165"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5165297.1"
                     /translation="MTSDADLVARVRKGDADAFAVLVARYRDRLGRYAVHMLGDRNDA
                     EEALQDAFVRAYRSLHRCREDDQFGRWLFGILVNRCRTAGRRSARRNRVFVRDEVSLE
                     QASAPQVPDHAEWEDAVRRAMQRLARPLREAFLLKHVEDMEYTEMSRVTGVGVSALKM
                     RVLRAREQLRTLLSEVERV"
     gene            871..1404
                     /locus_tag="AC16JC_11170"
     CDS             871..1404
                     /locus_tag="AC16JC_11170"
                     /inference="COORDINATES: protein motif:HMM:NF027875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HIR5165298.1"
                     /translation="MFDRDEDVVQRVIDHLRQPVEMDPTLDAAVLARVAAGDGRLSRL
                     GAAWEWLSRPRDIAVSPLAGFAAAALAGLALWIGVRSPRVVVPAPGPSTVQFVVRAPG
                     AGTVALVGDFNDWDGTVTPMRAAPNGALWSVTIPLTPGRYRYAFLVNGSRWIVDPAAP
                     RAQDDFDTPSSVVTVGG"
BASE COUNT          217 a          481 c          534 g          244 t
ORIGIN      
        1 ttgttggggg tggcgatcgt gacccaggtc ccggtagtgt cgagatcggt agtggcctcg
       61 atccggctca cgtagatatt cacgctggcg actgtatcga acgggaaagg gtcgtccgtg
      121 atcaggacct gcgtcgggct gatcgccgcc gggtgcagcg tgtcgtcctg gtaacaggcg
      181 gccgccacgg acccgagcag ggccagggag gcagcgaaca gatgcttcat ggaggctccg
      241 cggtgtcgag tggctcgaag gcgaataccc cgtgccccga accagggtca caatccggcc
      301 gggtgacccg acggccgcat gcccgggtat atgcaccggt gacgagcgac gccgacctgg
      361 tagcacgggt tcgaaagggt gatgcggacg ccttcgccgt gctggtcgcg cgctaccgcg
      421 accggctcgg ccgctatgcc gtgcacatgc tcggcgaccg caacgatgcc gaagaggcgc
      481 tccaagatgc attcgtgcgg gcgtaccgct ccctgcaccg ctgccgggaa gacgaccagt
      541 tcggtcgttg gctgttcggg attctggtga accgatgccg cacggccggc cggcgtagcg
      601 cccggcgcaa ccgcgtgttc gtgcgcgacg aggtgtccct cgagcaggcg agcgcacccc
      661 aagtgccgga ccacgccgag tgggaggatg cggtccgccg ggcgatgcag cgcctggccc
      721 gcccgttgcg ggaggcattt ctgctgaagc acgtggagga tatggagtac acggaaatgt
      781 cccgggtcac cggcgtgggt gtctccgccc tgaagatgcg cgtgctgcgc gcccgtgagc
      841 agctgcggac cctgctcagc gaggtcgagc gtgtttgacc gcgacgaaga cgtcgtgcaa
      901 cgggtgatcg accacctgcg ccagccggtc gaaatggacc cgacgctgga cgccgcggtg
      961 ctggctcgcg tggcggcggg cgacggtcga ttgagccggc tcggcgccgc gtgggaatgg
     1021 ctctctcgcc cgcgggacat cgcggtcagt ccgctcgcgg gcttcgcggc ggccgcgctc
     1081 gcgggactgg ccctgtggat cggcgtcagg tcgccccgcg tcgtggtgcc cgcacctggc
     1141 ccgagcaccg tccagttcgt ggtccgtgct cccggggccg gtaccgtcgc cctggtcggc
     1201 gatttcaacg attgggacgg aacagtgacg ccgatgcgcg ccgcccccaa tggagccctg
     1261 tggagcgtga cgattccgct gaccccgggc cgttaccgct atgcgttcct cgtcaacgga
     1321 tcccggtgga tcgtcgatcc ggcggcgcca cgcgcccagg atgacttcga tactccgagc
     1381 tccgtcgtga cggtgggagg atgatcggta tgcccaactc ccgcagaatt gccgtactgc
     1441 cactcgcgtt gagcctgctc gtgccgcgcg ccgcgg
//
LOCUS       DBNGZW010000477         6744 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1613646, whole genome shotgun sequence.
ACCESSION   DBNGZW010000477 DBNGZW010000000
VERSION     DBNGZW010000477.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6744)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6744)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6744
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1613646"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1102
                     /locus_tag="AC16JC_11175"
     CDS             <1..1102
                     /locus_tag="AC16JC_11175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009549520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5165299.1"
                     /translation="PHHLELGARVAAFATQEIAPRPEPADDAAARLEARELLGLLGRA
                     GWYTPIGEQHLRSCCLVREALAAAAPLADAVFALQALGTLPIILSGSDEQKRRWVGAA
                     LAGEAMAAFAMTEPEAGSDVAAIATTARRDGDTYVLDGMKTLISNAGIADFYVVFAST
                     DSAAGNKGLSTFLVPADAAGLHFVRPLVLAAPHPLGEIQLVGCRVPVANRLGAEGDGF
                     TLGLATLDRLRPTVGAAACGMGARALAESLRHAAERHQFGRPLAAQQLIQEKLARMAT
                     DLVAARLLVYRAAWEKDRGAERITTEAAMAKAFATEAAQRAVDDAVQILGGRGVLADH
                     PVDRLYRSVRSLRIYEGTTEILHLVIAGQLLK"
     gene            1099..3351
                     /locus_tag="AC16JC_11180"
     CDS             1099..3351
                     /locus_tag="AC16JC_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HIR5165300.1"
                     /translation="MKIVSIGGGPAGLYAAILLKKADPSHDVRVVERNRLDDTFGFGV
                     VFSEATQENLQQADPETFDAMARRSARWDDIEIHYRGELLRSGGHGFSGLSRRAMLEI
                     LARRATELGVRFEIGTEVTDVAPYLEADLVLAADGANSIVRDRFAATFRPTIDWRPNR
                     FVWLGTTRPFPAFTFYFKADRHGLWRVHAYQYEQGASTFIVEAREETWRAACLDRATE
                     DETIAFCEELFAEELAGHHLARNRSIWRSFGTVRNECWHRENIVLIGDAAHTAHFSVG
                     SGTKLAMEDAIVLVRALAAATDLPTALAAYEAERRPVVESLQRAAQVSLQFFENTERY
                     MDQEPVQFGFNLLTRSLRITHDNLKVRDAQFVAGVDRWFAGQAARQARVAVPLDPPPP
                     PLFTPYRLGELLLENRCVVSPMCQYCAEDGVPNDWHLVHLGTRALGGAALIFTEMTDV
                     TREGRISTGCTGMYKPEHVAAWNRIVEFVHRHTRAKIGIQLGHAGRKAATRKMWEGDN
                     EPLEEGAWPIMAASPIPYFPHSQVPREMDPADMHAVIDAYARATHMAQEAGFDALELH
                     YAHGYLLAGFISPLTNRRRDEYGGPLANRMRFPLAVFDAVRAAWPTAKPISVRISAVD
                     WAPGGMESQDAVDVARLLREHGCDILDVSAGQTVADQRPVYGRLFQTPFADRIRHEAG
                     MPVMTVGNISSFTDVNTILAAGRADLCVLARAHLWDPYWTRHAAQALGWPVEWPDQYH
                     TLNRYKPRFT"
     gene            3364..4146
                     /locus_tag="AC16JC_11185"
     CDS             3364..4146
                     /locus_tag="AC16JC_11185"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015048726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - L-tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006569 - L-tryptophan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HIR5165301.1"
                     /translation="MAVTYASYLRLDELLALQRPHEGELEHDETLFIIIHQVYELWFK
                     EVLHELDHLTRLLRDNATPQALHTLKRILTVLKVLVAQIDILETMTPLGFLSFRDRLE
                     SGSGFQSFQFRELEFVLGLKQASAVERYPDGSRHRERVERRYNEPGLWDAFLVFLQHH
                     GYTVPRALLERDLTRPIEPSPELQRVLVQVYRTDPGVTAVCERLVDLDEGFMEWRYRH
                     VKMVQRTIGTRHGTGGSAGADYLLTTLNRPAFPDLWAIRTEL"
     gene            4143..5348
                     /locus_tag="AC16JC_11190"
     CDS             4143..5348
                     /locus_tag="AC16JC_11190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020661262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase/PvdN C-terminal domain-containing
                     protein"
                     /protein_id="HIR5165302.1"
                     /translation="MTDTAQSLFASPNALAPHYSRFRVTERLLLTGHSHQAWPDVGFA
                     AQQQAWLDAAELVDDKWDRAFAMADRVRAGFARLLESPGGDIALGVNTHELVVRFLSA
                     LPLAKRPRLVTTDGEFHTVRRQLDRLHEAGIEVVRVPARPANQLAERLARMVTDRTAA
                     VLVSSVLYENAHIVPNLGFTLEACRRFGAELLVDAYHALNVVPFSLATERLDGAYVVG
                     GGYKYCQLGEGNAFLRVPPDCTLRPVITGWYSEFALLSSPPISGPVPYGPGPARFAGA
                     TYDPTSHYRAAAVFDFFEEQGLTPRLLRDVSQHQVGLLAHAFDHLDLDRTVIKRDRRS
                     VPLEATGGFLALYAPRAREISQALRRRNVWTDYRGDTLRLGPAPYLSDVQLRDAVRQL
                     QETVAAVGG"
     gene            5406..6641
                     /locus_tag="AC16JC_11195"
     CDS             5406..6641
                     /locus_tag="AC16JC_11195"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5165303.1"
                     /translation="MSESLRVVSGPQYRTGVTAAGERLARPQRYSSKLALVLPGGGAR
                     AAYQVGVLAALAERLPDLEFPILTGVSAGAINTIYLAAHPGQLAQAVAGLQVAWGRLT
                     TEQVFRVRPGSLVGAAARWFLASLLGRGTGPTPARGLLDTQPLREFLEATVDVRGIQA
                     NIDAGRLRTAALTATAINSGRSVTFIQGRSDEPAWEGLKHAGLRTPLRLEHVMASAAI
                     PILFPAVPLIGEFFCDGSVGQAAPLAPAIRLGARAAFVIGLKTRYRDARPAPATQPYP
                     SVAEVSGLLLDTIFQDQLEADADQLARINSLLAALPTHMPAPQALRPVDLLVLRPRRE
                     LATLSQGLSTTLPKQVRRIVDGIGGQREGAAEFLSYLLFEPEFTSQLVELGYEDLGAQ
                     WPEIERFFAKLERRADAPD"
BASE COUNT          974 a         2438 c         2306 g         1026 t
ORIGIN      
        1 cccccaccac ctggagctcg gcgcgcgtgt cgccgcgttc gccacgcagg agatcgcccc
       61 acggccggag cccgccgacg acgccgcggc acgcctcgaa gcccgcgagc ttctcggcct
      121 gctcggccgg gcgggctggt acacgcccat cggcgagcag cacctgcgct cctgctgcct
      181 cgtccgtgag gccctcgcgg ccgctgcgcc gctggccgac gccgtgttcg cgctgcaggc
      241 cctgggcacc ctgcccatca tcctgtccgg ctccgacgag cagaagcgcc gctgggtagg
      301 cgccgcgctc gccggtgagg cgatggccgc gttcgccatg accgaaccgg aagcggggtc
      361 cgacgtcgcg gccatcgcca ccacggcgcg ccgggacggg gatacctacg tgctcgacgg
      421 catgaagacg ctgatctcga acgccgggat cgccgacttc tacgtcgtgt tcgcgtccac
      481 cgacagcgcc gccggcaaca agggcctgtc cacgttcctc gtccccgctg acgccgccgg
      541 actccacttc gtgcggccgc tggtgctcgc tgcgccccac cccctggggg aaatccagct
      601 cgtcggctgc cgcgtccccg tggccaaccg cctgggcgcc gaaggggacg ggttcacgct
      661 cgggctcgcg acgctcgacc ggctccgccc cacggtaggc gccgccgcct gcggtatggg
      721 cgcgcgggca ctcgccgagt cgctgcggca cgccgcggag cgccaccagt tcggccggcc
      781 gctggccgcg cagcagctga ttcaggagaa gctcgcccgc atggcgacgg acctggtcgc
      841 cgcccggctg ctcgtctatc gcgcggcgtg ggaaaaggac cgtggcgccg aacggatcac
      901 gaccgaagcc gctatggcga aagccttcgc caccgaggcg gcgcagcgcg ccgtggacga
      961 tgcggtccag atcctcggcg gccgcggcgt cctggccgat caccccgtgg accggctgta
     1021 ccgctcggtg cgcagcctgc gcatctacga gggcacgacc gagatcctgc atctcgtgat
     1081 cgccggccag ctcctgaaat gaagatcgtc tccatcggcg gcgggccggc cgggttgtac
     1141 gccgccatcc tcctgaagaa ggccgatccg tcgcacgacg tgcgcgtcgt ggagcgcaac
     1201 cggctcgacg atacgttcgg cttcggcgtc gtcttctccg aggcgacgca ggagaacctc
     1261 cagcaggccg accccgagac gttcgacgcg atggcgcgcc gctccgcccg ctgggacgac
     1321 atcgagattc actaccgggg ggagctgctc cgctccggtg gccacgggtt ctcgggactg
     1381 tcgcgacgcg ccatgctcga gattctggcg cgccgggcga cggagctggg cgtgcgcttc
     1441 gagatcggca ccgaggtgac ggatgtcgcc ccctacctcg aggccgacct cgtgctggcg
     1501 gccgacgggg ccaacagcat cgtgcgcgac cgcttcgccg cgacgttccg tcccaccatc
     1561 gactggcgtc ccaaccgctt cgtgtggctc ggcaccacgc ggcctttccc cgccttcacg
     1621 ttctacttca aggccgaccg gcacgggctg tggcgtgtcc acgcctatca gtacgagcag
     1681 ggcgcctcga ccttcatcgt ggaggcgcgc gaggagacct ggcgcgcggc gtgtctcgac
     1741 cgcgccaccg aggacgagac gatcgcgttc tgcgaggagc tgttcgccga agagctcgcc
     1801 gggcaccacc tggcgcgcaa ccgctccatc tggcgcagct tcggtactgt gcgcaacgaa
     1861 tgctggcacc gcgagaacat cgtgctgatc ggcgacgcgg cgcacaccgc gcatttctcg
     1921 gtcggatcgg gcacgaagct cgccatggag gacgcaatcg tcctggtgcg ggcgctcgcg
     1981 gccgccaccg atctgcccac cgccctggcg gcgtacgaag cggagcgccg cccggtggtg
     2041 gagagcctgc agcgcgccgc tcaggtgagc ctgcagttct tcgagaacac cgagcgctac
     2101 atggaccagg agccggtcca gttcgggttc aatctgctca cccgctcgct acgcatcacc
     2161 cacgacaacc tcaaggtgcg cgacgcgcag ttcgtggccg gcgtggatcg ctggttcgcg
     2221 ggccaggccg cgcgccaggc gcgcgtcgcg gtgccgctcg acccgccgcc gccgccgctg
     2281 ttcacgccgt accggttggg ggagctgctg ctcgagaacc gctgcgtcgt ctctcccatg
     2341 tgccagtatt gcgccgaaga cggcgtgccc aacgactggc atctcgtcca tctcgggacg
     2401 cgcgcgctgg gcggcgcggc gctcatcttc accgagatga ccgacgtgac gcgcgaagga
     2461 cgcatctcga ccgggtgcac cggcatgtac aagccggagc acgtcgccgc ctggaaccgg
     2521 atcgtcgagt tcgtgcaccg gcacacgcgg gcgaagatcg gcatccagct gggccacgcc
     2581 gggcgcaagg ccgcgacaag gaagatgtgg gagggcgaca acgaaccgct cgaggagggg
     2641 gcgtggccga tcatggcggc gtcgccgatt ccctacttcc cgcacagcca ggtaccccgg
     2701 gagatggacc ccgcggacat gcacgcggtc atcgacgcct atgcgcgcgc cacgcacatg
     2761 gcccaggagg ccgggttcga cgcgctggag ctgcactacg cgcacggcta cctgctcgcg
     2821 gggttcattt cgccgctcac caaccgccgt cgcgatgaat acgggggccc gctggcgaac
     2881 cggatgcggt tccccctcgc ggtgttcgac gccgtccgcg ccgcctggcc cacggccaag
     2941 ccgatctcgg tgcgcatctc cgccgtggac tgggccccgg gcgggatgga gtcgcaggac
     3001 gcagtggacg tcgcgcggct gctccgcgag cacggctgcg acatcctcga cgtctcggcc
     3061 gggcagacgg tggccgacca gcgaccggtg tatgggcggc tgttccagac gccgttcgcc
     3121 gaccgcattc gccacgaggc gggaatgccg gtcatgaccg tgggcaacat ctccagtttc
     3181 accgacgtca acaccatcct cgccgccggc cgcgccgacc tgtgcgtgct ggcgcgggcg
     3241 catctgtggg atccctactg gacccgccac gccgcccagg cgctcgggtg gccggtggag
     3301 tggccggacc agtaccacac actcaatcgc tacaaaccgc gcttcaccta gggacgtgcg
     3361 atcatggccg tgacctacgc gagttacctg cggctcgacg agctgctcgc cctgcagcgt
     3421 cctcatgagg gggagctgga gcacgacgag acgctcttca tcatcattca tcaggtctac
     3481 gagctgtggt tcaaagaggt gctgcacgag ctcgaccacc tgacacggtt gttgcgcgac
     3541 aacgcgaccc cgcaggcact gcacacgctc aagcggattc tcaccgtgct gaaggtgctg
     3601 gtcgcccaga tcgacatcct ggagaccatg acccccctcg ggttcctctc cttccgggac
     3661 cggttggagt cgggcagcgg cttccagtcg ttccagttcc gcgagctcga gttcgtgctc
     3721 gggctgaagc aggccagcgc ggtggagcgc taccccgacg gcagccggca ccgcgagcgg
     3781 gtcgagcgcc ggtacaacga gccggggctg tgggatgcgt tcctcgtctt cctgcagcac
     3841 catgggtaca cggttccgcg ggcgctgctc gagcgcgacc tcacccggcc gatcgagccg
     3901 agtccggagc tgcagcgcgt gctcgtccag gtgtaccgca ccgaccccgg cgtcacggcg
     3961 gtgtgtgagc ggctcgtcga tctcgacgag gggttcatgg agtggcgcta tcgccacgtg
     4021 aagatggtgc agcgcacgat tgggacgcgg cacggcacgg gtggctccgc gggcgcggac
     4081 tacctcctga ccaccctcaa ccggcccgcc ttccccgatc tgtgggcgat tcgcaccgag
     4141 ctgtgaccga caccgcgcaa tcgctgttcg cgtcccccaa cgcgctggcg ccgcactact
     4201 cccgctttcg cgtcaccgag cgcctgctgc tgacgggcca ttcgcaccag gcgtggcccg
     4261 acgtcgggtt cgcggcgcag cagcaggcgt ggctcgacgc cgcggagctg gtggacgaca
     4321 aatgggaccg ggccttcgcg atggccgacc gggtgcgggc cggattcgcc cggctgctcg
     4381 agtcgccggg gggggacatc gccctcggcg tgaacacgca cgagctggtg gtgcgcttcc
     4441 tctcggcgct gccgctcgcg aagcggcccc gcctcgtgac caccgacggc gagttccaca
     4501 cggtccggcg ccagctcgac cggctgcacg aagcagggat cgaggtggtg cgcgtcccgg
     4561 cacggccggc gaaccagctg gccgagcggc tcgcacggat ggtgaccgac cggaccgcgg
     4621 cggtcctggt gtcgtccgtg ctgtacgaaa acgcgcacat cgtgccgaac ctggggttca
     4681 cactggaagc atgccgccgc ttcggcgcgg agctgctggt ggacgcctac cacgccctca
     4741 acgtggtgcc gttctccctt gcgacggagc gactggacgg cgcctacgtc gtcggcggcg
     4801 gctacaagta ctgccagctg ggcgagggca acgcctttct ccgtgtgcct ccggactgca
     4861 ccctgcgccc cgtgatcacg gggtggtaca gcgagttcgc gctgctttcc tctcccccga
     4921 tctcggggcc ggtcccctac ggcccgggac ccgcccgctt cgccggcgcc acctacgatc
     4981 ccacgagcca ttaccgggcg gccgcagtgt tcgacttctt cgaggaacag gggctcaccc
     5041 cgaggctgct gcgcgacgtc agccagcatc aggtgggatt gctggcgcac gcgttcgacc
     5101 atctggacct ggaccgcacg gtgatcaagc gcgatcgacg ctcggtgccg ctcgaggcga
     5161 cgggtgggtt cctggcgctc tacgccccgc gggcgcgcga gatctctcag gcgttgcgcc
     5221 ggcgcaacgt gtggaccgat tatcgcggcg ataccctgcg actcggcccg gctccctacc
     5281 tgagcgacgt ccagttgcgg gacgcggtcc ggcagctcca ggagacggtc gccgccgtcg
     5341 gcggctgaca acatgcggac cgcgcgcctt cgcggcgcct tgccgagcag tgcatcttga
     5401 gcgtcatgtc cgaatccctc cgggtggtgt cggggccgca gtaccgcaca ggcgtaacgg
     5461 ccgccggcga gcgactggcg agaccccagc gctattcctc caagctcgcc ctggtgcttc
     5521 ccggtggcgg cgcgcgggcg gcgtatcagg tcggggtgct cgccgccctg gcggaacggt
     5581 tgccggatct cgaattcccg attctcaccg gggtctccgc cggtgccatc aacacgatct
     5641 acctcgcggc ccatccggga cagctcgccc aggccgtcgc cgggctgcag gtcgcctggg
     5701 gacggctcac cacggagcag gtgttccggg tgcgtcccgg aagcctggtg ggagccgcgg
     5761 cgcgctggtt cctcgcctcc ctgctcggac ggggcacggg acccacgccc gctcgcggcc
     5821 tcctcgacac gcagccgctg cgcgaattcc tggaagcgac ggtcgacgtg cgcggcatcc
     5881 aggcgaacat cgacgccgga cggctgcgca ccgcggcgct cactgcgacc gcgatcaact
     5941 cgggccgctc cgtcacgttc atccagggcc gcagcgacga gccggcctgg gaaggactca
     6001 agcacgcagg cttgcgcaca ccgttgcggc tcgagcatgt catggcgtcg gcggcgatcc
     6061 cgattctgtt ccctgctgtg cccctgatcg gggagttctt ctgcgacggg agcgtcggcc
     6121 aggccgcacc gctcgcgccg gcgattcgcc tgggcgcacg ggcggcattc gtgatcgggc
     6181 tgaagacgcg ctatcgggac gcgcgccccg ctcccgccac gcaaccatat cccagcgtgg
     6241 cggaggtgtc cggcctgctg ctcgacacga tcttccaaga tcagctggaa gcggatgcgg
     6301 atcagctggc gcgcatcaat agcctgctgg cggcgctgcc gacgcacatg ccggcgccgc
     6361 aggcactgcg gccggtggac ctcctcgtgc tgcgcccgcg ccgcgagctg gccaccctct
     6421 cgcaggggct ctcgaccacg ctgccgaaac aggtgcggcg aatcgtcgac gggatcggcg
     6481 gccagcgcga aggggcggcg gagtttctca gctatctgct gttcgagccg gaattcacgt
     6541 cgcaactcgt ggagctcggg tacgaggacc tcggggcgca atggcccgag atcgagcgat
     6601 tctttgcgaa gctcgagcgc cgggccgacg cccctgacta gcttgccttc gacccgcccc
     6661 cggagtcgtc gagcatcctc ctgacggccg ccaggtagtc gtgcgccagc gcatgctgcg
     6721 gcgcataggc cgcccacacg ctgg
//
LOCUS       DBNGZW010000478         6543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_317141, whole genome shotgun sequence.
ACCESSION   DBNGZW010000478 DBNGZW010000000
VERSION     DBNGZW010000478.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6543
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_317141"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="AC16JC_11200"
     CDS             <1..653
                     /locus_tag="AC16JC_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165304.1"
                     /translation="NRISFVSAALLLVGGGLAAQQNDPDRTVAGGGVFPTGWNVRTER
                     NAPAAQVKFVAMGGGFHATMGPAAIMWRDADVATGNYHTVATFTQTKAPVHPEAYGLI
                     VGGQHLADSAQSYTYLIVRGAGEFSIWRRAGYANKPTAVVPWTANAAVHKADSAGAAT
                     NELAVQIKDGKASFTVNGTEVYSTADSNVDASGIVGYRVNHNLDVHLSAIGIHKLP"
     gene            772..1290
                     /locus_tag="AC16JC_11205"
     CDS             772..1290
                     /locus_tag="AC16JC_11205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165305.1"
                     /translation="MSNRWISAALAGALLGFSTPGASQTTHAMGPSSVARAAAHRDSL
                     PVEQLARVKQATARYRDVATAVADGYADINVVIPNMGRHYLKQALMDSTFDVEHPEIL
                     VYAPDSSGTLQLVAAEFAVPTNLSTKAPEGFRGSADQWFVNEQFNIWTLHAWVWKENP
                     AGIFYPTNPNVK"
     gene            complement(1358..2176)
                     /locus_tag="AC16JC_11210"
     CDS             complement(1358..2176)
                     /locus_tag="AC16JC_11210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5165306.1"
                     /translation="MVAAVGVVLSLLLPAPVVAQEPVAAGRLEAHVLYDSSYRRPRRV
                     WVYTPPGYDTSRAAPYPLIVAFDGDESRDTMPLPRVLDTLLAARRAPAFVAVLVDDSS
                     GAARIADLANAARMVDFLARQLLPWARRGWHVTRDPHRVIVTGSSAGGLAASCVAFAR
                     PDLFGNVWSQSGAFWRGAEASNTAPWEWLTQQVAAAPHKDVRFCLDVGELEDHATLGG
                     TGPNFRDATRRLRDALVAKGYDVTYTEVPGGNHAERWWRLRLPEGIVALSAAWH"
     gene            complement(2179..2694)
                     /locus_tag="AC16JC_11215"
     CDS             complement(2179..2694)
                     /locus_tag="AC16JC_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5165307.1"
                     /translation="MVITDAPSFLDYLSRIRGRTLAVARVIPQDRLEWRPTEGAFSFG
                     DLLRHLAAIERYMFAENARLLPARYPGHGRELADGYAAVFDYVDRMHAEAVAIFAALS
                     PADLQRRCVTPGGAEIPVWKWLRAMVEHEIHHRGQIYVYLAMLGIHGPPIYGLTSEEV
                     RTRSRTVKPEG"
     gene            complement(2696..4177)
                     /gene="pdxR"
                     /locus_tag="AC16JC_11220"
     CDS             complement(2696..4177)
                     /gene="pdxR"
                     /locus_tag="AC16JC_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070279 - vitamin B6 binding [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:2000142 - regulation of DNA-templated
                     transcription initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MocR-like pyridoxine biosynthesis transcription
                     factor PdxR"
                     /protein_id="HIR5165308.1"
                     /translation="MPKRAGTITLTLPPRDPGTPAYRWLSDALRSAILEGRLGPGVRL
                     PATRDLTGPYGLSRGTIVSAFEQLRSQGYISGRVGSGTYVNRILPEDLLHVRRDRGRA
                     SQPPAHRTAAYRLSDYGRRVRPFPGLTDPPPRAFRTDQPALDLLPTALWAQLAGRRLR
                     RPSPDLLLGCEALGYRPLREAVAVYLQASRGVRGGAHQIAIVSGTQQALDLVTRMLVN
                     PGDRVGIEDPGYPGAALVFEACGAHISTVPVDDEGMAIPDRRQRGVRLAYTTPGHQFP
                     IGVGMSLRRRMALLEWARASDALIFEDDYDSEFRYAGRPMPALQGLDRDGRVLFAGSF
                     SKVLFPSLRLGYLLVPEGLVDAVAASLSISTRHAPVLEQAVLCDFITQGHFGRHIRRM
                     REVYAERRAVLLEEAERHLGGLLEITGVEAGLQTAGWLAPGIREAAATRAAARRNVEV
                     TPLSRYTRGRAAREGLQLGFAAVAPPEIRRGVRELAAALGEAR"
     gene            4263..4733
                     /locus_tag="AC16JC_11225"
     CDS             4263..4733
                     /locus_tag="AC16JC_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020718358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR5165309.1"
                     /translation="MTRRFSYAKSAPGVFDAMDVLDQYVAACGLEESLLHLVRLRASQ
                     LNGCAYCIDMHWKDLRALGEGEQRLYELDAWRESPYYSPRERAALAWAEAVTLIAGAH
                     VPDAVYDAVRPHFNEKELCDLTMAVSAVNAWNRLSIASRLPAGTYEPAKVTSPR"
     gene            complement(4717..5280)
                     /locus_tag="AC16JC_11230"
     CDS             complement(4717..5280)
                     /locus_tag="AC16JC_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165310.1"
                     /translation="MTTTTQLRGLRVVVDNTRPDITTDDVLRRLDEALGLIERYAPHH
                     FRKLARAFDHILVERYACRGAYFPDQRACMVELTFTVNRDFTASQIAATILHEAMHAR
                     LHSLGFPLAMADRARQERFCRRAEIEFGLAVPDGAPIVERAMAAEQASDAEVAPAIDP
                     ALAARRVAQADREASLPAWLKRLTEGK"
     gene            complement(5339..5845)
                     /locus_tag="AC16JC_11235"
     CDS             complement(5339..5845)
                     /locus_tag="AC16JC_11235"
                     /inference="COORDINATES: protein motif:HMM:NF024102.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HIR5165311.1"
                     /translation="MTDPTGAGYLRLMTRPSDVALALAAAAAACGGESLLRSPLDSLR
                     LDVIAARTTIAVGDTTTVRFRLSNAGGDTVRLTFSSGCQVLPYVTDTDQLVVYPEGGG
                     WACITVITHLELAPGAQKVIPLVIHGGSPAAPGFANVPLEPGQYWMYATLEQPDHPRS
                     ESLLFTVR"
     gene            5911..6273
                     /locus_tag="AC16JC_11240"
     CDS             5911..6273
                     /locus_tag="AC16JC_11240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165312.1"
                     /translation="MTFKPSIWYPIAFVLSAVNLVAVGFAARPGEPWWHAGSHAALAL
                     AFGLWAQRLRQSRGVVGGNELQERLEALEAEVGRVEALEGDVGKLRQELSEAQERLDF
                     AERLLVRGREKDRVDPQR"
BASE COUNT         1016 a         2224 c         2335 g          968 t
ORIGIN      
        1 tgaaccggat ctccttcgtg tccgccgccc tgctgctcgt cggcggcggc ctggcggcac
       61 agcagaacga ccccgaccgc acggtggcgg gcgggggcgt gtttccgacg ggttggaacg
      121 tccggaccga acgcaatgcg cccgcggccc aggtgaagtt cgtcgccatg ggcggcggct
      181 ttcacgccac gatggggccc gcggccatca tgtggcgtga cgccgacgtg gccacgggca
      241 actaccacac ggtcgcgacg ttcacgcaga ccaaggcccc ggtgcatccc gaagcgtacg
      301 ggctgatcgt cgggggccag cacctggccg attcggcgca gtcgtacacc tatctcatcg
      361 tccgcggagc cggtgaattc agcatctggc ggcgcgcggg ctacgccaac aagccgaccg
      421 ccgtcgtacc ctggacggcg aacgccgcgg tccacaaggc ggacagcgcc ggagcggcca
      481 cgaacgagct tgccgtccag atcaaggacg gcaaggcgag cttcacggtc aacgggaccg
      541 aggtgtactc gacggccgac agcaacgtgg acgcgtcggg catcgtcggc tatcgcgtga
      601 accacaacct cgacgtgcac ctctcggcga tcgggatcca caagcttcca tagcgggcca
      661 ccggccgcgc gcgggaaccg tctcgcgcgc ggctcggttc ttctttcgat gccgatcgca
      721 tccgtgcgat cggtgtgccg gacccctttt ccatattcca ggagtgtacc gatgtcgaat
      781 cgttggatct ccgccgccct ggccggcgcg ctgctcgggt tctccacccc cggcgcctcc
      841 cagacgaccc atgccatggg gccatcgtcc gtcgcaaggg cggccgctca tcgcgattcg
      901 ctgcctgtcg agcagctggc gcgcgtgaag caggccaccg cgcgctatcg cgatgtcgcc
      961 accgccgtcg cggacggcta cgcggacatc aacgtggtga ttcccaacat ggggcgtcac
     1021 tacctgaagc aggcgctgat ggactccacg ttcgacgtgg agcatccgga gatcctcgtt
     1081 tatgcgcccg actccagcgg cacgctgcag ctcgtggccg cggaattcgc cgttcccacg
     1141 aatttgtcga cgaaagcccc cgaggggttc cgtggctcgg ccgatcagtg gttcgtgaac
     1201 gagcagttca acatctggac gctgcacgcc tgggtctgga aagagaaccc ggcggggatc
     1261 ttctatccga ccaatccgaa cgtgaagtga gctgatcgcg tgtggggccg gagctgctct
     1321 ccggccccgt tttctcttct cctgctctcc ggctgcttca gtgccaagcg gcgctcagcg
     1381 ccacgatccc ctcggggagc cgcaagcgcc accagcgctc cgcgtgattc cccccgggca
     1441 cctccgtgta agtcacgtcg taccctttcg ccacgagcgc gtcccggagg cggcgggtcg
     1501 cgtcccggaa attgggcccc gttccgccca gcgtggcgtg gtcttccagc tcccccacat
     1561 ccaggcagaa gcgcacgtcc ttgtgcggcg ccgcggcgac ctgctgcgtc agccactccc
     1621 agggagccgt gttcgacgct tccgcgccgc gccagaatgc gccgctctgc gaccacacgt
     1681 tgccgaacag gtcgggccgc gcgaaggcca cacacgacgc tgcgagcccg ccggcactcg
     1741 agccggtcac gatcacgcgg tgcggatcgc gcgtgacgtg ccacccgcgg cgcgcccagg
     1801 gcagcagctg tcgcgccaga aagtccacca tccgggcggc attggcgaga tcggcgatgc
     1861 gcgccgcgcc gctcgaatcg tccaccagaa cggccacgaa cgcgggcgcg cgccgggcgg
     1921 cgagcagcgt gtcgaggacc cgggggagcg gcatcgtatc gcgagattca tcgccgtcga
     1981 acgccacgat cagcgggtac ggcgcggcgc ggctcgtgtc gtacccgggc ggggtgtaca
     2041 cccagacgcg ccgcggacgc cggtaggagg agtcgtacag cacgtgcgcc tcgagccggc
     2101 cggcggcgac cggttcctgc gccacgaccg gcgcgggcag cagcagcgag agtaccacgc
     2161 caacggcagc gaccacggtc agccctccgg tttcacggtc cggctccggg tgcgcacctc
     2221 ctccgacgtg aggccgtaga tcggaggacc gtggatgccc agcatggcga ggtagacgta
     2281 gatctgccca cgatggtgga tctcgtgctc caccatggcg cggagccact tccagacggg
     2341 gatctcggcg ccgcccgggg tcacgcagcg gcgctgcagg tcggcggggg agagtgcggc
     2401 gaagatcgcc accgcttccg cgtgcatccg gtcgacgtag tcgaacaccg ccgcgtaccc
     2461 gtcggccagc tcgcgcccgt gccccgggta gcgcgcgggc aggagccgcg cgttttcggc
     2521 gaacatgtaa cgctcgatgg cggcgagatg acgcagcagg tcgccgaagg agaacgcgcc
     2581 ttcggtgggt cgccactcga gtcgatcctg cgggatgacg cgcgcgacgg ccagcgtgcg
     2641 gccgcggatc ctcgacaggt agtcgagaaa cgacggggcg tcggtgatca ccatgtcagc
     2701 gtgcctcccc cagtgccgcc gcgagctccc ggacgccgcg ccggatctcc ggcggggcga
     2761 cggcggcgaa ccccagctgt aggccttcgc gcgccgcacg cccgcgggtg tagcggctga
     2821 gcggcgtcac ctcgacattg cggcgcgcgg ccgcccgggt cgcggcggcc tcgcggatcc
     2881 cgggcgcgag ccaaccggcg gtctgcaatc cggcctccac gcccgtgatc tcgagcaaac
     2941 ccccgagatg ccgctcggcc tcctcgagga ggacggcccg gcgctccgcg tacacctctc
     3001 gcatgcggcg gatgtgacgt ccgaagtgac cctgggtgat gaagtcgcac agcaccgcct
     3061 gctcgagcac gggggcatgc cgcgtcgaaa tcgaaagcga ggccgcgacg gcgtccacca
     3121 gaccctcggg aaccaggaga taccccagcc gcagggaggg gaacagcacc ttgctgaagc
     3181 tgcccgcgaa cagcacccgg ccatcgcgat ccaatccctg cagcgccggc atcggacgac
     3241 cggcatagcg gaactcgctg tcgtaatcgt cctcgaagat cagcgcgtcc gacgccctgg
     3301 cccactcgag cagcgccatc cgccgccgca ggctcatgcc cacgccgatg ggaaattggt
     3361 ggcccggcgt cgtgtaggcc agtcgcactc cacgctggcg ccgatccggt atcgccatgc
     3421 cctcgtcgtc gaccggtacg gtggagatat gtgcgccaca ggcctcgaac acgagggcgg
     3481 cgccgggata gcccgggtcc tcgatgccca cgcggtcgcc ggggttcacg agcatgcgcg
     3541 tcacgagatc gagggcctgt tgggtgcccg agacgatggc gatctggtgc gccccgcctc
     3601 gtacaccgcg tgaggcctgg aggtagacgg cgacggcctc ccgcaacgga cggtagccca
     3661 gcgcctcgca tcccagcagc aggtccgggg acgggcgccg caggcggcga cctgcgagct
     3721 gcgcccagag tgcagtgggg aggagatcca aagcgggttg atcggtccgg aatgcgcgcg
     3781 gcggggggtc cgtcaatccg gggaaggggc gcacccgccg cccgtagtcc gagaggcggt
     3841 acgcggccgt ccggtgcgcc ggcggctgcg acgcccggcc gcggtcgcgg cggacgtgaa
     3901 gcaggtcctc ggggaggatc cggttcacgt acgtccccga tccgacgcgg ccgctgatgt
     3961 acccctgcga cctgagctgc tcgaaggcgc tgacgatcgt gccgcgcgac aggccatagg
     4021 gtccggtgag gtcgcgggtg gcggggaggc gcacgccggg accgaggcgg ccttccagga
     4081 tggcgctgcg caaggcgtcg ctgagccagc ggtaggctgg ggtccccggg tcgcgcggcg
     4141 gcagcgtcag ggtgatggtt ccggcgcgtt tgggcatcgt aagtggtaca gtcaaaatat
     4201 caaaaatggc gcttctcgaa agttccactt gcgattaggc tctggcgtca caggaggagc
     4261 acatgacgcg acgtttcagc tacgccaagt cggccccggg cgtgttcgac gccatggacg
     4321 tgctggacca gtacgtggcc gcctgcggcc tggaggaatc gctgttgcac ctcgtgcggc
     4381 tgcgggcctc gcagctcaac gggtgtgcgt attgcatcga catgcactgg aaggacctgc
     4441 gggccctggg agaaggagag cagcggctgt acgagttgga cgcctggcgt gagagcccgt
     4501 actacagccc gcgcgagcgc gccgcgctcg cctgggcgga ggcggtcacc ctgatcgccg
     4561 gcgcccacgt ccccgacgcg gtctacgacg cggtgcgccc gcatttcaac gagaaggagc
     4621 tgtgcgacct gacgatggcg gtgagtgcgg tcaacgcctg gaatcggctg tcgatcgcgt
     4681 cgcgcctgcc cgccgggacc tacgagcccg cgaaggtcac ttcccctcgg tgaggcgctt
     4741 gagccaggcc ggcagggacg cttccctgtc ggcctgcgcc acgcgccggg cggccagcgc
     4801 cggatcgatg gcgggagcca cctcggcgtc cgacgcctgc tccgcggcca tggcgcgctc
     4861 gacgatgggg gccccgtcgg gaacggcgag gccgaactcg atctcggccc ggcggcagaa
     4921 tcgctcctgg cgcgcgcgat ccgccatcgc cagggggaat cccaggctgt gcaggcgggc
     4981 gtgcatcgcc tcgtgcagga tcgtggccgc gatctgggac gcggtaaaat cacggttgac
     5041 cgtgaaagtc agctcgacca tgcaggcgcg ctggtcgggg aagtacgcgc cgcggcaggc
     5101 gtagcgctct accaggatgt ggtcgaacgc ccgtgccagc ttgcggaagt ggtgcggggc
     5161 gtagcgctcg atcagcccca gcgcctcgtc gaggcggcgc agcacgtcgt ccgtcgtgat
     5221 gtcgggtcgg gtgttgtcga ccacgacccg gaggccgcgc agctgcgtgg tggtcgtcat
     5281 cgtactcaag ttgtcgcggg ccactcccgt gggcaaccgc agcccctggg gggcgggttc
     5341 agcgcaccgt gaagagcagc gactcggatc gtgggtggtc gggctgttcc agggtggcgt
     5401 acatccagta ctgcccgggc tcgagcggca cgttggcgaa ccccggggcc gccggggacc
     5461 cgccgtggat cacgagcggg atgacctttt gcgcgccggg ggccagctcg aggtgtgtga
     5521 tcacggtgat gcaggcccag ccgccgccct ccgggtacac gaccagttgg tcggtgtcgg
     5581 tgacgtacgg gagcacctgg cagccgctgc tgaaggtgag gcggaccgtg tcacctccgg
     5641 cgttgctgag ccggaaccgg accgtggtcg tgtcgccgac ggcgatcgtg gtgcgggcgg
     5701 cgatcacgtc caggcgcagc gagtcgagcg gggagcggag gaggctctcg ccaccgcagg
     5761 cggcggccgc ggctgcgagc gccagggcaa cgtcggaggg cctggtcatc aggcggagat
     5821 accccgcgcc ggtcgggtcg gtcacatctg atatcctccg ctccggccga ttgacaccaa
     5881 gcgacatgtc gccacgttta gtaggtcgcc atgaccttca aaccgtccat ctggtacccg
     5941 atcgcctttg tactgagcgc cgtcaacctg gtcgccgtcg ggttcgcggc ccggccgggt
     6001 gagccgtggt ggcatgccgg gtctcacgcg gcgctcgcgc tggcgttcgg gctctgggcc
     6061 cagcggctgc gccagtctcg tggggtagtc ggcggcaacg agctgcagga gcggctcgag
     6121 gcgctcgagg ccgaggtggg ccgggtcgaa gcgctcgagg gcgacgtggg caagctgcgc
     6181 caggagctga gcgaggcgca ggagcggctc gacttcgccg aacggctgct ggtgcgggga
     6241 cgggagaagg accgggtgga cccgcagcgc taaggtgagg tgagtcgagc ggaggtctgc
     6301 cctcccgtcc atcaacatta ccttccagcg ttgttgacca actcagatcg atgaggccca
     6361 gtttcgatgt tgcgattggg tgacacctcg acgcgggtta gcttggaggc atgataccca
     6421 acgttagccg cctcgcgcgc tgcggcgcgc tcgtcccact ggcgttcgcg tcgctcgcgg
     6481 ccaacgcccg cgcgcagcaa ccattcgaca ccaccacgtg gaccatcaga gccgccgaat
     6541 cgc
//
LOCUS       DBNGZW010000479         4275 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_523719, whole genome shotgun sequence.
ACCESSION   DBNGZW010000479 DBNGZW010000000
VERSION     DBNGZW010000479.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4275)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4275)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4275
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_523719"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="AC16JC_11245"
     CDS             complement(<1..1493)
                     /locus_tag="AC16JC_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5165313.1"
                     /translation="MRSFTDTFIKHPVLAAVVNLVILLVGWRAMSSLPIQQYPKIESS
                     SVLITTVYYGASAETVRGFLTTPIERAVSAISGVDYVESTSRAGVSTITVHLKLNHSS
                     TAALAEVTARLQQVRSELPAEAEPPVVEVQRADRPYASFYLSFTSSDRDIPALTDWLT
                     RNMQPRLTTLAGVQRVDVVGGRPIAMRIWIDPDRLAALNLAPGDVHAALQRNNYLAAV
                     GQTKGDLVQVNLLANTDLRSPEEFKNLVVAERNGAIVRLSDVARVELGAEEADFITKF
                     NDKEAVYLGVWPLVGTNEIELAHRLKDAMNDIRPTLPRDIDMQLAYDATVFMEDALHE
                     ISKTLAETILIVGLVVFLFMGSVRTALVPLVAMPVSLVGAAIVMSAFGFSLNLLTILA
                     IVLSVGLVVDDAIVVVENVERHVREGKTRIQAALVGARELVGPILAMTVTLAAVYTPI
                     GFQGGLTGSLFLEFAITLAAAVVVSGIVAVTLSPVMSSRFVHEHGREG"
     gene            complement(1506..2612)
                     /locus_tag="AC16JC_11250"
     CDS             complement(1506..2612)
                     /locus_tag="AC16JC_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5165314.1"
                     /translation="MRSPFLLKNRQWAASAVLLLILTVTGGGLVAWKRASIKAANAAA
                     ASQPEPMQSVTSAVARETRYRATATSIGTVLALNSITLRNELPGTVRRVALSPGQIVE
                     TGTVLVALDVSVEQADLEALQAQADLAQTTLTRLERLRQAQAASQEEVDQARAARDVA
                     LAQVARTKAIIARKTLRAPFRARVGLADVHIGQYLNEGTELTTLQGVDAAADVDFTVA
                     QAVAAQLRPGEVVRVVAGGEPQPIAAHIVAIDARVDPTTRNALVRARIPGHAPSPGAS
                     VRVEVPAGAPAQAVAIPVSALRKGPGGDHVFVIVTDSTGRSRARLQPVQSGAVLEDEV
                     LILQGLAPGARVATSGSFKLRDGALVAAAGDSLR"
     gene            complement(2783..3106)
                     /locus_tag="AC16JC_11255"
     CDS             complement(2783..3106)
                     /locus_tag="AC16JC_11255"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5165315.1"
                     /translation="MSTRTAAPKALTSREFELVAKALSDERRMALLEAIGSAAEYPCH
                     QLCEQFPVSKGTISHHMKELLRAGLIRARREGQYLHYEVRRDTIEAYTNELMRRLGRP
                     GAAES"
     gene            3255..3977
                     /locus_tag="AC16JC_11260"
     CDS             3255..3977
                     /locus_tag="AC16JC_11260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165316.1"
                     /translation="MLRCPLEMPGGHLILRHDAHTLLLDTGSPVSMGRRPTYHFLDRE
                     IPVLLRYQGMTMEQWSENIGVNIDALLGCDVLGRHAVTIDPEAGHVVFDEDPPPSCEC
                     VAPLQTVAGMPVVEVGLAGRRLRALFHTGATLSCLRDVDARAQRCIGVARDCYPGLGE
                     FTTELRRVTLMFGDLPVSLDCGLLPPAVEQALRPVEVHAIVGTNLLQAFTVGWGPRFS
                     ELRLVALPFVAPVRVDRLEVIH"
     gene            complement(3974..>4275)
                     /locus_tag="AC16JC_11265"
     CDS             complement(3974..>4275)
                     /locus_tag="AC16JC_11265"
                     /inference="COORDINATES: protein motif:HMM:NF013857.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR5165317.1"
                     /translation="TPVVVEVETDTQLDEALAAGVRHILVDNQTPERVAAWVRRAGRG
                     VTVQASGGITHQTARAYADAGAHLIAIGELTHSVPAAPIRCDILAPPGASSLGPA"
BASE COUNT          669 a         1424 c         1494 g          688 t
ORIGIN      
        1 ccttcacggc cgtgctcgtg cacgaagcgc gagctcatca cgggcgacag cgtgaccgcg
       61 acgatccccg acaccacgac cgccgcggcg agggtgatcg cgaactcgag gaagagcgac
      121 ccggtgagcc cgccctggaa cccgatcggc gtgtacaccg cggccagcgt gaccgtcatc
      181 gccaggatcg gcccgaccag ctcgcgcgcg ccgacgagcg cggcctggat gcgcgtcttg
      241 ccttcgcgga cgtgccgttc gacgttctcc accacgacga tcgcgtcgtc caccaccagg
      301 cccaccgaga gcacgatcgc gaggatcgtg agcaggttca ggctgaagcc gaaggccgac
      361 atcacgatcg cggcgccgac cagcgacacc ggcatcgcga ccaggggcac cagcgccgtg
      421 cgaaccgacc ccataaagag gaagacgacc aggccgacga tcaggatggt ctcggccagc
      481 gtcttgctga tttcgtgcag cgcgtcttcc atgaagaccg tcgcatcgta ggccagctgc
      541 atgtcgatgt cccgaggcag cgtgggccgg atgtcgttca tcgcgtcctt gagccggtgc
      601 gccagctcga tctcgttggt gccgaccagc ggccagacgc cgagatacac ggcctccttg
      661 tcgttgaact tcgtgatgaa gtcggcctcc tcggcgccga gctccacccg ggcgacgtcc
      721 gacaaccgca cgatcgcccc gttccgctcg gcgacgacga ggttcttgaa ttcctcgggc
      781 gagcgcaggt cggtgttggc gagcaggttg acctgcacca ggtcgccctt ggtctggccg
      841 acggccgcga ggtagttgtt gcgctgcagc gcggcgtgca cgtcgccggg ggcgaggttg
      901 agcgcggcca ggcggtccgg gtcgatccag atccgcatgg cgatgggacg tccgcctacg
      961 acgtccacac gctgcacacc ggcgagcgtg gtgagccggg gctgcatgtt ccgggtcagc
     1021 caatcggtga gcgccgggat atcccggtcg ctcgaggtga agctgaggta gaacgaggcg
     1081 tagggccggt cggcgcgctg cacctcgacc accggtggtt cggcttccgc cggcaactcg
     1141 gagcgcacct gctgcaggcg ggccgtgacc tcggccaggg ccgccgtgct ggagtggttc
     1201 aacttgaggt gcaccgtgat cgtgctgacg ccggcccggc tcgtcgattc gacgtagtcg
     1261 acgccgctga tggccgacac ggcgcgctcg atcggcgtcg tcaggaatcc ccggacggtc
     1321 tcggccgagg cgccgtagta gaccgtcgtg atcagcaccg aggagctctc gatcttgggg
     1381 tactgctgaa tcggcagcga ggacatcgcc cgccagccga cgagcaggat gaccaggttc
     1441 accaccgccg cgaggacggg gtgcttgatg aacgtgtcgg taaacgagcg catgtccgta
     1501 gtccgttaac ggagtgaatc gccggcggcc gcgaccagtg caccgtcgcg gagcttgaag
     1561 gaccccgaag tggcgacgcg cgcaccgggg gcaagaccct gcagaatcag cacctcgtcc
     1621 tcgagcaccg caccgctctg caccggttgc aggcgtgcgc ggctgcggcc ggtcgaatcg
     1681 gtgacgatca cgaagacgtg atcgccgccg ggccctttgc gcagcgcgct gacgggaatg
     1741 gcgacggcct gggcgggcgc ccccgcggga acttcgaccc gcaccgacgc accgggcgac
     1801 ggggcgtggc ccggaatgcg ggcgcgcacc agcgcgttgc gggtcgtggg gtcgacccgc
     1861 gcgtcgatcg ccacgatgtg cgccgcgatc ggctggggct cgccgccggc cacgacccgg
     1921 accacctcac ccggccgcag ctgggcggcc accgcctgcg cgaccgtgaa gtccacgtcg
     1981 gccgctgcgt ccacaccctg gagcgtggtg agctcggtgc cctcgttcag gtattgcccg
     2041 atgtggacgt cggcgaggcc gacgcgggca cggaacggag cgcgaagcgt cttgcgggcg
     2101 atgatcgcct tcgtgcgcgc aacctgcgcc aatgcgacgt cgcgcgccgc gcgcgcctga
     2161 tccacctctt cctgggaagc ggcttgcgcc tgccgcagac gctccagccg ggtcaaggtg
     2221 gtttgcgcca ggtccgcctg cgcctgcagc gcctcgaggt cggcctgctc caccgagacg
     2281 tcgagcgcga cgagtacggt gccggtttcg acgatctggc cgggcgagag cgcgacccgg
     2341 cgaaccgtgc cgggcagctc gttgcgcagg gtgatcgaat tcagcgccag gaccgtgccg
     2401 atcgaggtgg cggtcgcgcg gtaccgggtc tcgcgggcga ccgcgctcgt caccgattgc
     2461 atcggctccg gctggctggc ggccgcggcg ttggcggcct tgatggaggc gcgcttccag
     2521 gcgaccaggc cgccccccgt gacggtgagg atgagcagca ggaccgcgga tgcggcccat
     2581 tggcgattct tcaggaggaa tggactgcgc atacaggtcg ggctccttag ggtcgagaaa
     2641 ggtcctgctc gatgcgtcca gctctagacg tttggccgcg ccgggattcg acagtcggac
     2701 gaccgggtcc accgattcct gacattcgta ttacagtttt ataactatcg aacaacacgg
     2761 gcaaaaaaaa agagttcccg cgtcacgact ccgcagctcc tggccgtccc agccgccgca
     2821 tcaattcgtt ggtgtacgcc tcgatcgtgt cgcggcggac ctcataatgg aggtactgtc
     2881 cttcgcggcg ggccctgatc aggccggcgc gaagcaactc cttcatgtga tgggagatag
     2941 tgcccttgga gaccgggaac tgttcgcaca gctggtgaca cggatactcc gccgcgctgc
     3001 cgatggcctc gagcagcgcc atgcgccgct catcggacag ggccttggcg accagctcga
     3061 attccctcga ggtgagggct ttcggggcgg cggtgcgcgt ggacatggtt cgatatttat
     3121 cgaactggcg attcctgtca agcgcgcgag ggtgacttgg cccggcaggg ttactgcagg
     3181 acctcccccg cactcttcct aggagcggtc tgacccgttt gtgcaggcgc ccggcgatcc
     3241 acgtgatgac tttcatgctc cgatgcccac tggagatgcc cggcggccat ctcatcctgc
     3301 gacacgacgc ccatacgctg ctcctggaca ccggctcccc ggtctcgatg gggcgccgcc
     3361 ctacctacca cttcctcgat cgcgagattc ccgtactcct gcgctatcag ggaatgacca
     3421 tggagcagtg gagcgagaac atcggcgtca acatcgacgc gttgctcggg tgcgacgtgc
     3481 tcggcagaca tgccgtcacg atcgatcccg aggccggcca cgtcgtgttc gacgaagacc
     3541 cgccaccgtc ctgcgaatgc gtcgccccgc tgcagaccgt cgccgggatg ccggtggtcg
     3601 aggtcgggtt ggccgggagg aggctgcggg cgctgtttca cacgggcgcc acgctgtcgt
     3661 gcctgcgcga cgtcgatgcc agagcgcagc ggtgtatcgg ggtggcccgg gactgctacc
     3721 cgggactggg agagttcacc acggagctgc gacgggtgac gctgatgttc ggagacctgc
     3781 cggtcagcct cgattgcggg ctgctgccgc cggccgtcga gcaggcgctg cgcccggtgg
     3841 aagtgcacgc catcgtggga acgaatttat tgcaggcgtt caccgtgggg tggggtcccc
     3901 gcttcagcga gttacggctg gtggcgctgc ccttcgtggc gcccgttcgc gtcgatcgac
     3961 tcgaagtgat tcattaggct ggcccgagcg aggaggcgcc cgggggggcg agaatgtcgc
     4021 accggatggg agccgcgggc accgaatggg tcagctcccc gatcgcgatc aggtgcgcgc
     4081 cggcatccgc gtaggcgcgc gcagtctggt gggtgatgcc gcccgaagcc tggaccgtca
     4141 cgccgcggcc ggcgcgccgc acccaggccg ccacccgctc gggcgtctga ttgtcgacca
     4201 ggatgtggcg cacccccgca gcgagcgcct cgtcgagctg agtgtcggtc tccacctcga
     4261 cgacgaccgg cgtgc
//
LOCUS       DBNGZW010000480         1378 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1679343, whole genome shotgun sequence.
ACCESSION   DBNGZW010000480 DBNGZW010000000
VERSION     DBNGZW010000480.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1679343"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..991)
                     /locus_tag="AC16JC_11270"
     CDS             complement(<1..991)
                     /locus_tag="AC16JC_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5165318.1"
                     /translation="MADLLDRLRASLADRYTFDREIGRGGMATVYRAEDVKHHRPVAI
                     KVLHPYLAMNLGSERFLREIQIAAQLQHPHIVPLYDSGQAGDLLYYVMPFVEGESLRQ
                     RLEREPRLSLEEVLHLARSIAAALDYANRHGVVHRDIKPENVMLHEGEAMVTDFGIAK
                     AVSVAAADNLTQTGTAVGTPAYMSPEQAGGADELDGRSDVYSLGCVVYEMLTGRTPFT
                     GPTAQAIIAQLFTEPPPPLRSVRSAEGTPEWAEQAVMKALAKDPAARFTTAAQFSQAL
                     KASGVSTPPAATVTAAAGPATKSIAVLPFVNMSADPENEYFTDGIAEEIINALT"
     gene            complement(1001..>1378)
                     /gene="fusA"
                     /locus_tag="AC16JC_11275"
     CDS             complement(1001..>1378)
                     /gene="fusA"
                     /locus_tag="AC16JC_11275"
                     /inference="COORDINATES: protein motif:HMM:NF012882.7"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HIR5165319.1"
                     /translation="DSNEMSFKMAGILAFRNVSPNCKPVLLEPIMDVEVWTPDDYQGA
                     VMGDLSSRRGQILGTEPDGRLVKVKALVPEADLDRYATSLHSITHGRGTYRQKFHTYE
                     QVPPEVAHKVVDARKKELEEEKA"
BASE COUNT          201 a          488 c          449 g          240 t
ORIGIN      
        1 tcgtgagcgc gttgatgatc tcttcggcga tcccgtcggt gaagtactcg ttctccgggt
       61 cggcgctcat gttgacgaac ggcagcacgg cgatcgattt cgtggccgga ccggctgcgg
      121 ccgtcacggt cgccgcgggg ggcgtggaga cacccgacgc cttgagcgcc tgggagaact
      181 gcgccgcggt ggtgaaccgc gcagccgggt ccttcgcgag cgccttcatc accgcctgct
      241 ccgcccactc gggcgtgccc tccgccgacc ggaccgagcg cagcggtggc ggcggctcgg
      301 tgaacagctg ggcgatgatc gcctgcgccg tcggacccgt aaacggcgtg cgacccgtca
      361 gcatctcgta cacgacacac cccagactgt atacgtcact gcggccgtcc agctcgtccg
      421 ccccgcccgc ctgctcggga ctcatgtacg ccggggtacc caccgcggtg ccggtctggg
      481 tcaggttgtc cgccgcggcg acgctgaccg ccttggcgat cccgaagtcg gtcaccatcg
      541 cctcgccctc gtgcagcatc acgttctcgg gcttgatgtc gcgatgcacc accccgtggc
      601 ggttggcgta gtcgagcgcc gcggcgatcg agcgggccag gtgcagcacc tcctcgagcg
      661 acaaccgggg ctcgcgctcg agccgctggc gcagcgactc gccttccacg aacggcatga
      721 cgtaatagag caggtcgccc gcctgccccg aatcgtagag cggaacgatg tgcgggtgct
      781 gcagctgcgc ggcgatctgg atctcgcgca ggaagcgctc tgagccgagg ttcatggcca
      841 gatagggatg gagcaccttg atcgccaccg ggcggtggtg cttcacgtcc tccgcccggt
      901 acaccgtggc catcccgccg cgcccgatct cgcgatcgaa cgtgtagcgg tcggccaggc
      961 tcgcccgcaa acgatccagc aggtcggcca tcggtgcggc ttacgccttc tcctcttcca
     1021 gttccttctt gcgcgcgtcg accaccttgt gggcgacctc gggcggaacc tgctcgtagg
     1081 tgtggaattt ctgccggtag gtgccgcgcc cgtgggtgat cgagtgcaac gacgtggcgt
     1141 agcggtcgag gtccgcctcc ggcaccagcg ccttcacctt caccagacgc ccgtcgggct
     1201 ccgtgccaag aatctggccg cggcgcgagg agagatcgcc catcaccgcc ccctgatagt
     1261 catccggtgt ccacacctcc acgtccatga tcggctcgag cagcaccggc ttgcagttcg
     1321 ggctcacgtt ccggaacgcc aggatgcccg ccatcttgaa cgacatctcg ttcgagtc
//
LOCUS       DBNGZW010000481         2454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_993502, whole genome shotgun sequence.
ACCESSION   DBNGZW010000481 DBNGZW010000000
VERSION     DBNGZW010000481.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_993502"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="AC16JC_11280"
     CDS             complement(<1..829)
                     /locus_tag="AC16JC_11280"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5165320.1"
                     /translation="MQDLRYALRQLARSPSFTLAAVLTLGLGIGATTAIFSVVDGVLL
                     RPTPFVDMDRLAMVWETDRKSGTSHEPASVPDFQDFQRRSGRFAQLAAFAAGEVNLTP
                     TQGEPARLAALAVSHELLPMLGVRPLVGRTFTADEDAPGNAARVVLISDELWQRAFGR
                     DRDVVGRTLRIDDTPWTVIGVLSAGAEFGTLQVLRAADYSRGFADRGGQARVDAWVPL
                     RADPVSLPRDTHPIFVVGRLAADASMSTAQQELAGIAADLERTYPVNDARGVSIQPLT
                     "
     gene            complement(926..>2454)
                     /locus_tag="AC16JC_11285"
     CDS             complement(926..>2454)
                     /locus_tag="AC16JC_11285"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR5165321.1"
                     /translation="RREMAIRQALGAARGRLVRQLLTESVLLALAAGAVGILFASWGL
                     RALITIWPNALPNASEIALDGPVLAFTVGLALLTGLAFGLVPAWRASAPGIEEALRDE
                     AAGAIGGRRRRLQGGLVVGEIALALVLLVGAGLLVRSFIRLSSVNPGYDTRNLLAARI
                     RLTPARYPNEAGQAQFFANVLTALATRPGVQNASIAGTLPLSGSVRIWAFDPRTVRPD
                     FPEPFMVLRSFAVSPDYFSAFRIPIRRGRAFTADDRAGAPRAAVMNRAATDQLWPGQD
                     PVGKQFTFRWPGGPSGGGTMTIVGVIDNLRSTSLDAEPQPEIYTPVAQDADLSEMWVV
                     LRAANGRPLQLAGAIRDAVRQADPEQPIGEIASLSEMSDRLTAARRFNTTLLTLFACL
                     AVVLALVGIYGVTAYAVTQRAREMGIRMALGAQPGQVVGLLVRENLRRVALGVVLGLA
                     AAFGVTRVLKALLYEVTPSDAVTFAATAVLLATVALAATWWPARRATRVDPVVALRTE
                     "
BASE COUNT          382 a          893 c          861 g          318 t
ORIGIN      
        1 gtgtcagtgg ctggatcgaa acgcctcgcg cgtcgttcac cgggtaggtg cgctcgagat
       61 cggcggcgat cccggccagc tcctgctggg ccgtgctcat actcgcatcg gcggccagcc
      121 gtcccaccac gaagatcggg tgcgtgtcgc gcggcaagct caccggatcc gcgcgcagcg
      181 gtacccacgc atccacgcgc gcctgcccgc cgcggtcggc gaagccgcgc gagtaatccg
      241 ccgcccggag cacctggagc gtcccgaact ccgcgccggc tgacagcacg ccgatgacgg
      301 tccagggggt gtcgtcgatg cgcagcgtgc gccccacgac gtcccgatcg cgcccgaacg
      361 cccgttgcca caactcgtcg ctgatcagca cgacccgggc ggcgtttccc ggcgcatcct
      421 cgtccgcggt gaacgttcga cccacgagcg gccgcacgcc gagcatgggc aggagctcgt
      481 ggctcacggc gagggcggcg agtctggccg gctccccctg cgtcggggtg aggttcactt
      541 cgcccgcggc gaacgccgcc agctgggcga accgtccgct gcgccgctgg aaatcttgga
      601 aatcggggac ggatgcgggc tcgtgggacg tcccgctctt gcggtccgtc tcccacacca
      661 tcgccagccg gtccatatcg acgaacggcg tcggccggag cagcacgccg tccacgacgc
      721 tgaagatggc ggtcgtggcg ccgatgccga ggccgagcgt cagcacggcg gcaagggtga
      781 agctcggact ccgggcgagc tggcggagcg cgtagcggag gtcttgcatg agagcttcca
      841 ttgtgcctcc agaagcaggt gtcaggtgcc gggcgtcagg tgccagaaac gccccccctg
      901 acacccgaca cctggcaccc gatacctatt cagttcgcaa cgccaccacc ggatctaccc
      961 gcgtcgcccg gcgggccggc caccacgtgg ccgcgagcgc gacggtcgcg agcagcacgg
     1021 cggtcgccgc gaaggtcacc gcgtcactgg gtgtgacctc gtagagcaga gccttgagga
     1081 cccgcgtgac gccgaaggcg gcggccaggc cgagcaccac cccgagcgct acccgccgca
     1141 ggttctcgcg caccagcagt ccgacgacct ggcccggctg ggcgcccagc gccatgcgaa
     1201 tccccatctc gcgcgcgcgc tgcgtgaccg cgtacgccgt gacgccgtaa atgcccacca
     1261 gcgcgagtac cacggccaga caggcgaaca gcgtgagcaa agtggtgttg aatcgtcgcg
     1321 ccgcggtcag ccggtcggac atctcgctca ggctggcgat ctcgccgatg ggctgctcgg
     1381 gatccgcctg gcgcacggcg tcgcggatgg cgccggcgag ctgcagcggc ctgccgttgg
     1441 ccgcccggag cacgacccac atctcggaca gatccgcatc ctgggcgacc ggcgtgtaga
     1501 tctcgggctg cggctcggcg tcgagcgagg tcgagcgcag gttgtcgatc acgcccacga
     1561 tcgtcatcgt tccacctccg ctagggcctc cgggccatct gaaggtgaac tgcttgccga
     1621 ccggatcctg ccccggccac agctggtcgg tggccgcccg attcatgacg gcggcgcgcg
     1681 gagcgcccgc gcgatcgtcg gcggtgaacg cgcggccgcg ccggatgggg atacggaacg
     1741 ccgagaagta gtccggagac actgcgaacg agcgcagcac catgaacggc tcggggaagt
     1801 ccggccggac ggtgcgcgga tcgaaggccc atatgcggac cgaccccgag agcgggaggg
     1861 tgcccgcgat gctcgcgttc tggacaccgg gtcgcgtcgc gagcgcggtg agcacattgg
     1921 cgaagaactg ggcctgtccc gcctcgttcg ggtagcgcgc cggcgtgagc cggatccggg
     1981 ccgccagcag gttgcgcgtg tcgtagccgg gattcacgct gctcaagcgg atgaaactgc
     2041 ggaccaggag acccgccccg acgagcagca ccagcgcgag cgcgatctcg ccgaccacca
     2101 gtcctccctg caaccggcgc cgccggccgc cgatggcgcc cgcagcctcg tcacggagcg
     2161 cctcctcgat tccgggcgcc gacgcccgcc aggccggcac gaggccgaag gcgagcccgg
     2221 tcaggagggc caggccgacc gtgaacgcca gcaccggccc gtccagcgcg atctcgctcg
     2281 cgttggggag cgcgttgggc cagatcgtga tgagcgcgcg cagtccccag ctggcgaaga
     2341 ggatgcccac cgcccccgcg gccagcgcga gcagcacgct ttcggtcagc agttgccgca
     2401 ccagccggcc gcgagcggcg cccagcgcct gccggatcgc catctcacga cgcc
//
LOCUS       DBNGZW010000482         1309 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_442024, whole genome shotgun sequence.
ACCESSION   DBNGZW010000482 DBNGZW010000000
VERSION     DBNGZW010000482.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1309)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1309)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1309
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_442024"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="AC16JC_11290"
     CDS             complement(<1..224)
                     /locus_tag="AC16JC_11290"
                     /inference="COORDINATES: protein motif:HMM:NF045352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165322.1"
                     /translation="MITDPTTATPKAERAATVACQFCSTLNRVALDRVGQGPKCGSCG
                     RPILLDRPLHVTDETFQQVLQGTDIPVLVD"
     gene            complement(259..870)
                     /locus_tag="AC16JC_11295"
     CDS             complement(259..870)
                     /locus_tag="AC16JC_11295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165323.1"
                     /translation="MTRLLRGTIGALAAAGVLAIIRLGSFTPYTAERDVGAIVRLAWR
                     ARGQHVRECRELTPEELERLPVHMREKEVCEGRLLPYRLLVAVDGAQLVDRLVHGAGA
                     REDRPLYVYEDLVVQPGTHRVVITFTLAASPPAHGEAEEAHEAEEPGERREADTHGQP
                     RQAATPPRLALDTTLELGSRRVLLVTYNEEREQLVVRGGSGGS"
     gene            complement(867..>1309)
                     /locus_tag="AC16JC_11300"
     CDS             complement(867..>1309)
                     /locus_tag="AC16JC_11300"
                     /inference="COORDINATES: protein motif:HMM:NF014692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hydrogenase iron-sulfur subunit"
                     /protein_id="HIR5165324.1"
                     /translation="GGGDVVVVACERGAGGATAAAALDGAVVYPVSCGGNLHTSVVEY
                     LLRAGAGGVLIVSCPPRDCWNREGPKWLEARLFHDREAELRERVDRRRVRLVHAAAGE
                     RAVVRAALAGFRADLTALEAATAESDIDLDRLCELVEEGVEQKT"
BASE COUNT          203 a          503 c          406 g          197 t
ORIGIN      
        1 aagtccacca gcacgggaat gtcggtgccc tgcagcacct gctggaacgt ctcatcggtg
       61 acgtgcagcg gacggtcgag caggatggga cgcccgcacg agccgcactt cggtccctgg
      121 ccgactcggt cgagcgccac gcggttcagg gtcgaacaga attggcacgc gacagtcgcc
      181 gcccgttcgg ctttcggagt cgcggtcgtg ggatcggtga tcatgatgat atcctcagaa
      241 tgtcgtccgt tttgcctatc aactaccgcc tgaaccgccg cgcaccacca actgttcccg
      301 ctcctcatta taggtcacca acaatacgcg gcgggagcca agctccagcg tcgtatcgag
      361 cgcgagtcgc ggtggcgtcg cggcttgccg cggttgccca tgtgtgtccg cctcgcgacg
      421 ttcgccaggt tcctccgcct cgtgcgcttc ctctgcttcc ccgtgggccg gcggcgacgc
      481 ggcgagcgtg aaggtaatga caacacgatg tgtgcccggc tgcacgacca gatcctcgta
      541 cacgtacagt gggcggtcct cgcgcgcgcc cgcgccgtgc accaggcgat cgacgagttg
      601 cgcgccgtct accgccacca ggagccggta ggggagcaac cggccctcgc acacttcctt
      661 ctcgcgcatg tgaaccggca gtcgttccag ctcctcggga gtcaactccc ggcattcacg
      721 cacgtgctga cctcgcgccc gccaggcgag ccgcacgatc gcccccacgt cgcgctcggc
      781 cgtgtacggc gtgaacgacc ccaggcggat gatcgccagg acgccggccg cggccagcgc
      841 tccgatcgtg ccgcgcagca agcgagtcat gtcttctgct ccacgccttc ctccaccagc
      901 tcgcacagcc ggtcgagatc gatgtccgat tcggccgtcg ccgcctcgag cgcggtgagg
      961 tcggcccgga aaccggccag cgccgcccgc accacggcgc gctcgcccgc cgcggcgtgc
     1021 accagccgga cgcggcggcg atccacgcgc tcgcgcagct ccgcctcccg gtcgtgaaac
     1081 agccgcgcct cgagccactt cggtccctcg cgattccagc aatcgcgcgg cggacaggag
     1141 acgatcagca cgccgcccgc gcccgcgcgc agcaggtact ccaccacgga ggtgtgcagg
     1201 ttaccgccac agctcaccgg atagaccacc gccccgtcga gtgccgccgc ggcggtcgcg
     1261 ccacccgccc cgcgctcgca cgctaccacg acgacgtcgc ccccacccg
//
LOCUS       DBNGZW010000483         3437 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1397035, whole genome shotgun sequence.
ACCESSION   DBNGZW010000483 DBNGZW010000000
VERSION     DBNGZW010000483.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1397035"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="AC16JC_11305"
     CDS             complement(<1..270)
                     /locus_tag="AC16JC_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HIR5165325.1"
                     /translation="MLIKRPDDIPSSEITPEELYWNRRDFIRAAGTIVGAALLPLPTP
                     RSLGWRGGWGDEEKPNSYDDITHYNNYYEFGSDKRDPSENSDGFHP"
     gene            complement(408..1562)
                     /locus_tag="AC16JC_11310"
     CDS             complement(408..1562)
                     /locus_tag="AC16JC_11310"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR5165326.1"
                     /translation="MHTARRTHSFTESVIRAMTRLAAQHDAINLAQGFPNFPAPQVLK
                     DAAARAIAADINQYAITWGAKHLRDAVARKYADWYGMTVDPETEITVTCGATEAMASA
                     LLAIVDPGDEVIVFEPFYENYGPDAILCGAKPVYVPIAPDQPLDLDRLRAAFSDRTRA
                     IVLCTPNNPTGKVFSAAELEGIAECCRRHDSYAVTDEIYEHIYYEGKHIPIATLEGMR
                     TRTITVSGASKTFSITGWRIGTIVAPPGTSDAIRKVHDFLTVGAPAPLQEGVAVAIET
                     LGRDYYEGLARDYRARRDLLCGALSAAGFRCTPPAGAYYVMADFSDLSDLPDDRFAAW
                     LTAEHGVAPVPGSSFYSRPELGRTVVRFAFCKTMDMLQEAAGRLRAVGGR"
     gene            1624..>3437
                     /locus_tag="AC16JC_11315"
     CDS             1624..>3437
                     /locus_tag="AC16JC_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165327.1"
                     /translation="MRPSPLAVRPRVLQGLAVVALALALTTCAREATGPLRRATFSVT
                     PVLPSGLSLAAFNLAVDNVRLIVVRPPADTVFDDVFTFPVNQSSLSISASVPLQQSPE
                     TFQVTLQLLSGTTPLFAGSQAVTVTAGDTPTPTPIPTGTYIGPGQNVASLTIGPLDSV
                     LTQGGTLQFRLTAKDAQGAIVPTFYASWTTSDTSVAKVDPTGVLTAPFRRATLTVTAR
                     TPNNVSASTPVTFAPVATTLTILSGCGQSGLLGTQLPQPIVAKVTAGDGLGVRGVAVT
                     FTAPAGGSVANAQVLTDSAGVAGTLVTLGSQSGPVAFQVSAPGLTAQQCAQSAFGAAA
                     QLAFTVQPTAALAGAVIAPAVVVAAQDAQGTLVPTFTGTVTIAVGLNPGNGALSGTTT
                     VAAVGGLATFSDLSIDKAGAGYTLVANATSLTGATSAAFNVAAGSASRLLFTVQPVTT
                     PAGTAITPAVVVTALDSLGNTVTTFAGTVTVSIDSGPAGAVLTGTPAVSAIAGVATFA
                     DLTLDKVGSYHLRAHATGLTDAISARCDVTIGLSRLLSFTVQPTSVTAGSVITPPVQV
                     TVKDLQGNTDATFNGTVVVAIATNPGGGTLSGIDTVAA"
BASE COUNT          485 a         1232 c         1151 g          569 t
ORIGIN      
        1 gggatgaaag ccgtcggagt tctccgacgg gtcccgcttg tcggacccga actcgtagta
       61 gttgttgtag tgcgtgatgt cgtcgtagga gttgggcttc tcctcatccc cccagccacc
      121 ccgccacccc agggagcggg gagtggggag tgggagcagc gctgcaccga cgatcgtacc
      181 tgccgcccgg atgaagtccc gccggttcca gtacagctcc tcgggtgtga tctccgagga
      241 gggaatgtcg tcgggtcgct tgatgagcat gggagccgtt tctgtgagcg tgtcttctat
      301 gaatgtacgc cacgccttcc gggttccgat gctgagaacg ttctaatgct gtccgccttt
      361 gtccgccttc ttccgccttc ttccgtcctt gttcgccctt tccgccctca ccgtcctccc
      421 accgcccgca accgccccgc cgcctcctgc agcatgtcca tggtcttgca gaaggcgaac
      481 cgcaccacgg tccgccccag ttccggccgg ctgtagaagc tcgagccggg caccggggcg
      541 accccgtgct cggccgtgag ccaggcggcg aagcggtcgt cggggaggtc ggagaggtcg
      601 gagaagtccg ccatgacgta gtatgcgccg gcggggggcg tgcagcggaa cccggccgcg
      661 gagagcgcgc cgcacagcag gtcgcggcgg gcgcggtagt cgcgcgccaa tccttcgtaa
      721 tagtcccgcc ccagcgtctc gatcgcgacg gccacgcctt cctgcagcgg cgccggggcg
      781 cccaccgtga ggaagtcgtg caccttgcgg attgcgtcgc tcgtcccggg gggggccacg
      841 atcgtcccga tgcgccaccc ggtgatgctg aacgtcttgg aggcgccgct cacggtgatg
      901 gtgcgcgtcc gcatcccctc gagcgtggcg atggggatgt gctttccttc gtagtagatg
      961 tgctcgtaga tctcgtccgt cacggcgtag ctgtcgtgcc ggcggcagca ctccgcgatt
     1021 ccctccagtt ccgccgccga gaacaccttc cccgtcgggt tgttgggggt gcagagcacg
     1081 atggcccggg tccggtccga gaaggcggct cgcagccggt cgaggtcgag cggctgatcg
     1141 ggagcgatgg ggacgtagac cggtttcgcg ccgcacagga tcgcgtccgg gccgtagttc
     1201 tcgtagaacg gctcgaacac gatcacctcg tcgccgggat cgacgatggc cagcagggcg
     1261 gaagccatcg cctcggtggc gccgcaggta acggtgatct ccgtttcggg gtccacggtc
     1321 atgccgtacc agtcggcgta cttccgcgcg acggcgtcgc gcaggtgctt ggcgccccac
     1381 gtgatggcgt actggttgat gtcggccgcg atggcccggg ccgcggcgtc cttcaggacc
     1441 tggggtgccg ggaaattggg gaacccctgc gccaggttga tcgcgtcgtg ctgggctgcg
     1501 agccgagtca tcgcgcggat gacggactcg gtgaaggaat gcgtgcggcg agccgtgtgc
     1561 atggggccca atgataacct gcttgacggc ctccaacttg caggctcaat tggctcggtc
     1621 ctcatgcgtc cgtctcccct ggccgtccgg ccgcgagtgc tccagggcct cgccgtcgtc
     1681 gcgctggccc tcgcgctcac cacctgcgcc agggaagcca cgggaccgct gcgccgcgcc
     1741 acgttttcgg tcacccccgt gctgccgagc ggtctgagcc tcgccgcgtt caacctcgcg
     1801 gtcgacaacg tccggctcat cgtggtgcgc ccgcccgcgg acaccgtgtt cgacgacgtg
     1861 ttcacgttcc cggtgaatca gtcgagtctc tccatctccg ccagcgtccc gctccagcag
     1921 tctcccgaaa cgttccaggt cacgctgcag ctgctctcgg gcacgacgcc gctgttcgcg
     1981 gggtcgcagg cggtgaccgt caccgccggc gacacgccga cccccacgcc gatcccgacc
     2041 ggcacctaca tcgggccggg gcagaacgtc gcctcgctga ccatcggtcc gctcgactcc
     2101 gtgctcaccc agggcggcac gctgcagttc cgcctgacgg cgaaggacgc gcagggcgcg
     2161 atcgtaccga cgttctatgc gtcctggacc acgagcgaca cgagcgttgc gaaagtcgat
     2221 ccgaccgggg tgctcaccgc gcccttccga cgggccacgc tgaccgtcac cgcgcggacg
     2281 ccgaacaacg tctcggcttc cacgccggtc accttcgcgc ccgtggcgac cacgctcacc
     2341 atcctgagcg gctgcggtca gagcggcctg ctcggtaccc agctgccgca gcccatcgtc
     2401 gccaaggtga cggcgggtga tgggctcggc gtgcgcggcg tcgcggtgac cttcacggcg
     2461 cccgcgggag gctcggtcgc caacgcgcag gtgctcaccg actcggccgg cgtggccgga
     2521 accctcgtca cgctcgggtc ccaatccgga ccggtcgcgt tccaggtcag cgcgccggga
     2581 ctcacggcgc agcaatgcgc ccagagcgcc ttcggcgccg ccgcgcaact ggcattcacc
     2641 gtccagccca cggccgccct cgcgggagcc gtcatcgccc ccgctgtcgt cgtcgcggca
     2701 caggacgcgc agggcacgct ggtgccgacc ttcaccggca ccgtcacgat cgcggtgggg
     2761 ctcaacccgg ggaacggcgc gctgtccggc acgaccaccg tggccgcggt cggcgggctc
     2821 gcgacgtttt cggacttgag catcgacaag gccggagcgg gctacacgct cgtggccaat
     2881 gccaccagcc tcaccggggc cacgagcgcc gcgttcaacg tggcggccgg ctccgcgtcg
     2941 cggctcctgt tcacggtcca gcccgtcacc acacccgccg gcaccgccat cacaccggcg
     3001 gtcgtggtca ccgccctgga ctcgctcggc aatacggtga ccacgttcgc gggcaccgtg
     3061 acggtgtcga tcgacagcgg gccggcgggg gcggtgctga ccggcactcc cgcagtgagc
     3121 gcgatagcgg gcgtcgcgac cttcgccgat ctcacgctcg acaaggtcgg gtcctatcac
     3181 ctccgtgccc atgccacggg gctgacggac gccatcagtg cgcgctgcga cgtcaccatc
     3241 gggttgtcgc gcctgctctc cttcaccgtc cagccgacca gcgtgacagc cggaagcgtg
     3301 atcaccccgc cggtccaggt gaccgtgaag gatctgcagg gaaatacgga cgcgactttc
     3361 aacggcaccg tcgtcgtggc catcgccacc aaccccggag gggggacgct ctcgggcatc
     3421 gacaccgtcg ccgccat
//
LOCUS       DBNGZW010000484         3286 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1206250, whole genome shotgun sequence.
ACCESSION   DBNGZW010000484 DBNGZW010000000
VERSION     DBNGZW010000484.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3286)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3286)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3286
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1206250"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..1112)
                     /locus_tag="AC16JC_11320"
     CDS             complement(69..1112)
                     /locus_tag="AC16JC_11320"
                     /inference="COORDINATES: protein motif:HMM:NF016292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5165328.1"
                     /translation="MTSRTDPMDSICSPEMPRRDFMAAITGGLLAAPLAAGAQQPAKV
                     PRIGYLITGSLESPETRALLDVFRQGLRERGHVEGGNILIEYRSADGNVERLPDLATE
                     LVRLKVDLIVAGATPAARAARQATKTIPIVASSMGDPVGDGLVASLARPGGNLTGTTF
                     LGPKLVPKHLELLRESLPRISLVAVLWHPGAFAESTTSELFKEAAAAGKALRLRLHLV
                     EARRPDEIDPGFSRMTREHPDALVVFPSPMLFAERRRIVALAARHRLPSMFNNRQAVE
                     LGGLIGYGANIPELQRQVADYADKILRGARPADLPVEQPTKFELVINLKTAKALGLTV
                     PPSFLQRADQVIE"
     gene            1634..2134
                     /locus_tag="AC16JC_11325"
     CDS             1634..2134
                     /locus_tag="AC16JC_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Csu type fimbrial protein"
                     /protein_id="HIR5165329.1"
                     /translation="MNRTARVALIFGGLVFGLAAPALAQTATANLSVTATVAKNCSIT
                     TTAVAFGSYDPIVANATTPLDGTGSVVVTCTKGAGTRIDLGLGSNAAASVRRMLGGTD
                     LLTYELYTDTGRSTVWGTGAGAGQTIAAAPNKNPRTFTVYGRVPAGQDVGAASYADTV
                     VATINF"
     gene            2469..2969
                     /locus_tag="AC16JC_11330"
     CDS             2469..2969
                     /locus_tag="AC16JC_11330"
                     /inference="COORDINATES: protein motif:HMM:NF017079.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Csu type fimbrial protein"
                     /protein_id="HIR5165330.1"
                     /translation="MIRTARVALILGGVVLGMAAPALAQTASTNMAVSATVAKNCSIT
                     TTAVAFGSYDPVVANASTPLDGTGTVVVTCTKGAGTRIDLGLGANASGAVRRMSGGTD
                     FLTYELYQNTTRTTIWGSGAAAGQTIVAAPSKAARTFTVYGRVAAGQDVAAASYNDTV
                     VATINF"
     gene            3029..>3286
                     /locus_tag="AC16JC_11335"
     CDS             3029..>3286
                     /locus_tag="AC16JC_11335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis chaperone"
                     /protein_id="HIR5165331.1"
                     /translation="MALGAAALLLLLAATTVAPRAARAADFTVNPIQIFLSAQTQSAI
                     LTIQNTSTEPLRFQLNAFAWAQDTAGQMQLTPTTEVIFFPRL"
BASE COUNT          514 a         1153 c         1107 g          512 t
ORIGIN      
        1 tgaacctgcg tcgttcgggc atcaccttgg tctccttttt cagaaggcga cggtgtagga
       61 gatatcgatc actcgatcac ctgatccgcc cgttgcagga acgacggcgg gaccgtcagg
      121 ccgagagcct tggcggtctt gaggttgatc accagctcga acttcgtggg ctgctccacc
      181 ggcaggtctg caggtcgggc gcctcggagg atcttgtcgg cgtaatccgc gacttgccgt
      241 tgcagctcgg gaatgttcgc cccataaccg atgaggccgc ccagctccac ggcctgccgg
      301 ttgttgaaca tcgacggcag gcggtgtctc gccgcgaggg cgacgatgcg ccgtcgctcc
      361 gcgaagagca tcgggctcgg gaacacgacg agggcatccg ggtgctctcg ggtcatcctc
      421 gagaatccgg ggtcgatctc gtcggggcgc cgcgcctcca cgagatgaag gcggagccgt
      481 agcgctttgc cggcggcggc ggcctctttg aacagctcgc tcgtcgtgct ctcggcgaat
      541 gcgccaggat gccacaggac cgcgacgagg gagatccgag gcagactttc cctgagcagc
      601 tccaagtgct tgggtaccag cttcggaccc aggaaggtgg tccccgtgag attcccaccg
      661 ggtcgggcga ggctggccac gaggccgtct ccgaccgggt cgcccatgga ggacgcgacg
      721 atggggatcg tcttggtcgc ttgccgcgcg gcgcgggcag ccggggtggc cccggcgacg
      781 atgagatcga ccttgagacg cacgagctcg gtcgccagat ccgggagacg ctccacgttc
      841 ccgtccgccg agcggtattc gatcaggatg ttgccacctt cgacatggcc ccgctcgcgt
      901 agtccttggc ggaagacgtc gagcagcgcc cgcgtctcgg gtgactcgag gctaccggtg
      961 atcagatagc cgatccgtgg gaccttcgcc ggctgctgcg ccccggcggc gagcggggcg
     1021 gcgaggaggc cgccggtgat ggccgccatg aagtcgcgtc ggggcatctc gggtgagcag
     1081 atagagtcca tcgggtcggt cctgctcgtc atcgccgcga ggtctccttt cgtccacctg
     1141 atcggtcgcg gactacggta ggcggcgcgc gcgccccctg tcaatcacac ccgggggctc
     1201 gtcgtcggag agccaggccg cggggcttgc acccgcatgc cggtggcgat ctcgacgtga
     1261 ggggcgaggg gaccgacgcg gggactcgcc gggcacgaaa ggacgcgatc ggccctctga
     1321 atccacgtga cgtcgaacgt cgtgctcgta ctcacgggtg agtggacggc gcgcggcccc
     1381 cgcacctcca cgaattgtgg cgcggagcct tcgaggctcc gcagcgtgaa gctccgcgcg
     1441 ggcctcgggc ctcaccgccg ggctcgcacg cacttcgtcc gtgttcgatt cgggacgcca
     1501 gtccggcacg ttggactgca cgcgccacgc gaggccgtcg tccgcgcggg cggtggcaca
     1561 cgtgttgccc ttactcctcc ccgtacgcac tgtgtggccg cgattgcggc ccgatcatcg
     1621 aggaggagac gatatgaacc gcacggctcg agtcgcactg atcttcggcg gactggtctt
     1681 cggtctggcc gcacccgcgc tggcccagac ggccaccgcc aacctctccg tcaccgccac
     1741 cgtcgccaag aactgcagca tcaccaccac cgcggtcgcc ttcggcagct acgatccgat
     1801 cgtcgccaac gccacgaccc cgctcgacgg caccggcagc gtggtcgtga cctgcacgaa
     1861 gggcgcgggc acgcggatcg acctgggcct gggcagcaac gcggcggcca gcgtgcggcg
     1921 catgctgggc ggcaccgacc tgctgaccta cgagctctac accgacaccg gccgcagcac
     1981 cgtctggggc acgggcgccg gcgcgggcca gaccatcgcc gcggccccca acaagaaccc
     2041 ccggacgttc acggtctacg ggcgcgtccc ggccggccag gacgtcgggg ctgcttcgta
     2101 tgcggatacg gtcgtcgcca ccatcaactt ctgatcgagc cgcgccgcac ctcgggagcg
     2161 ccggtcggcc gaatggccac cggcgctttc gcctttccag aaaccgacct acgagaaatg
     2221 gaggagaccc cctgcgcccc ctctgtttgg aggcttcccg ggcccatccg aggtggaatc
     2281 ccggctctga acggtcccga aagtaggctg gattccgacg tctttctgat aggttgtggc
     2341 ccgagacggt cctctcatcc cgccgggcgg gttgctggca ccggtgttgc cctagtagtg
     2401 ggcgtgtgca gcaccggagg ccgcgacggg cggccgcaat cttcaaatac atactgggag
     2461 gaaccgacat gatccgcacc gctcgagtag ccctgatcct cggtggagtg gttctcggca
     2521 tggccgcccc cgccctggcc cagaccgcgt cgacgaacat ggcggtgagc gccacggtgg
     2581 ccaagaactg cagcatcacg accaccgcgg tcgcgttcgg cagctacgat ccggtcgtcg
     2641 ccaacgccag caccccgctc gacggcaccg gcaccgtcgt ggtgacctgc acgaagggcg
     2701 cgggcacccg gatcgacctc ggcctgggcg ccaatgcctc aggtgcggtc cgccggatga
     2761 gcggcggcac cgacttcctg acctacgagc tgtaccagaa caccacccgg accacgatct
     2821 ggggctcggg cgcggcggcc ggccagacca tcgtcgcggc ccccagcaag gcggcccgca
     2881 ccttcacggt ctacgggcgc gtcgcagccg gccaggatgt ggccgcggcg tcctataatg
     2941 acacggtggt tgcgaccatc aacttctagc ccggaggact gacgcccgcg tgcagcaccg
     3001 acgatcagcg agcgcaggga gagcgccgat ggccctgggg gccgcggcgc tcttgctgct
     3061 gttggcggcc acgaccgtcg ccccccgcgc ggcgcgggcg gcggacttca cggtcaaccc
     3121 gatccagatc ttcctgtccg cccagacgca gagcgccatc ctcaccatcc agaacacgag
     3181 caccgagccc ctccgcttcc agctcaacgc ctttgcgtgg gcgcaggaca ccgccgggca
     3241 gatgcagctc acgccgacca ccgaggtcat cttcttcccg cgcctg
//
LOCUS       DBNGZW010000485         1877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1545762, whole genome shotgun sequence.
ACCESSION   DBNGZW010000485 DBNGZW010000000
VERSION     DBNGZW010000485.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1545762"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..384
                     /locus_tag="AC16JC_11340"
     CDS             <1..384
                     /locus_tag="AC16JC_11340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015256616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF933 domain-containing protein"
                     /protein_id="HIR5165332.1"
                     /translation="SASVEAEIAQMDPADRAAFLDDLGLKESALTRFIRTAYGLLDLI
                     SFFTVGPDEVRAWPIRRGATARAAAGRIHSDLERGFIRAEVIPYAVFMRYGSEQAVKD
                     AGLLQIEGKDHVVADGDILHIRFNV"
     gene            565..1863
                     /locus_tag="AC16JC_11345"
     CDS             565..1863
                     /locus_tag="AC16JC_11345"
                     /inference="COORDINATES: protein motif:HMM:NF017519.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX/YebB-like N1pC/P60 family cysteine
                     hydrolase"
                     /protein_id="HIR5165333.1"
                     /translation="MRGEDCRRTLARSNQAFAALGATLGAIATGLVDPDAPILDSLES
                     GFFALAPGVAACPADLPRYVEFYERVREVVKWQSRHWDVKTIAARRRLYRALYGGRAA
                     VEEVMLQHPDDVVTLLPGVREPSAAPGAMVYGVEIHSGDLLVSRGGYPTSALIARGND
                     YPGNFSHVGLVHVDSISHAVSVIQAHIERGVTVSSAEDYLRDKKLRIMVLRARFDLPR
                     MMADPLLPHRAATRALERARAGHIPYDFAMDYTDPSRLFCSEVASWPYHDLGLTLWMG
                     ISTISRPGLRRWLSAFGVRHFETEEPSDLEYDPQLVVVAEWRDPETLFRDHVDNAVID
                     AMLEGAERGDRLTYAWYTLPLGRLAKAYSWALEHFGRVGPVPEGMSAAAALRNRAFGI
                     RQRRLATRVDQGALLLAQTRGYPPPYWTLLELARQEAARR"
BASE COUNT          256 a          663 c          657 g          301 t
ORIGIN      
        1 tcggccagcg tggaggcgga aatcgcgcag atggatcccg ccgatcgcgc cgccttcctc
       61 gacgacctgg gactcaagga gtcggcgctg acgcggttca tccggaccgc gtacggcctg
      121 ctcgacctca tctcgttctt caccgtcggt ccggacgaag tgcgcgcctg gccgatccgt
      181 cgcggcgcca cggcgcgggc cgcggccggc cggatccatt cggacctgga acgcgggttc
      241 atccgggccg aggtgatccc ctacgccgtc ttcatgcggt acgggtccga gcaggcggtg
      301 aaggacgccg gcctgttgca gatcgagggc aaggaccatg tcgtggcgga cggggacatc
      361 ctgcacatcc gtttcaacgt ctagccaggg gagcccgggc cgattcgcgc tctcgccgta
      421 gtcctcggcg cgctggccgt cgcgttcctc gcgctggcca tcccgtcagc tgagccgccg
      481 cccgcaccgg cgccggagca cggcgccacg ttcgagtgga atcgggatgc ccagtggcgg
      541 agcctcgagg cagagtacgc ccgggtgcgc ggcgaggact gccgccggac gctcgcacgc
      601 tcgaaccagg cgttcgcggc gctcggtgcc acgctggggg cgatcgccac cgggttggtc
      661 gaccccgatg cgccgatcct cgattccctc gagtccggct tcttcgctct cgcacccggc
      721 gtcgccgcgt gcccggcaga cctgcctcgc tacgtcgagt tctacgagcg cgtgcgggaa
      781 gtcgtcaaat ggcagtcacg ccactgggac gtgaagacca tcgccgcccg gcgccggctg
      841 taccgggcgc tgtacggcgg gcgggccgcc gtcgaggaag tgatgctgca gcatcccgac
      901 gacgtggtca cgctgttacc cggcgtgcga gagccgtcgg ccgccccggg cgccatggtc
      961 tacggcgtgg agatccactc gggcgacctg ctcgtgtcgc gcggcggcta tcccacgtcc
     1021 gcgctgatcg cccgcggcaa cgactacccg ggcaacttct cgcacgttgg actggtccac
     1081 gtcgattcga tctcgcacgc cgtgtcggtg atccaggcgc acatcgagcg cggtgtgacg
     1141 gtgtcgtccg cggaggatta cctccgcgac aagaagctgc ggatcatggt gctgcgcgcc
     1201 cgtttcgacc tgccccggat gatggcggac cccctgctgc cgcatcgcgc ggcgacccga
     1261 gcgctcgagc gggcgcgggc cggtcacatc ccctacgatt tcgcgatgga ttatacggat
     1321 ccgtcgcgcc tgttctgctc cgaggtcgcg tcctggccgt accacgatct cggcctgacc
     1381 ctgtggatgg ggatctcgac gatttcgcgc ccggggctgc ggcgctggct cagcgccttc
     1441 ggcgtgcggc acttcgagac cgaggagccg tcggacctgg agtacgatcc gcagctcgtg
     1501 gtggtggcgg agtggcgcga cccggagacg ctgttccggg atcacgtgga caacgcggtc
     1561 atcgacgcga tgctcgaggg agccgagcgg ggcgaccgcc tcacctacgc ctggtacacg
     1621 ctgccgctgg gacgcctcgc caaagcgtac agctgggcgc tcgagcactt cggccgcgtg
     1681 ggacccgttc cggaaggaat gtccgccgcc gcggccttgc gcaaccgtgc cttcgggatc
     1741 cggcagcggc gcctcgcgac gcgcgtggat cagggcgcgc tgctgctcgc gcagacgcgc
     1801 ggctatccgc ctccctactg gaccctgctc gagctggccc gtcaggaagc cgcccggcgc
     1861 tgactcagcg caggaac
//
LOCUS       DBNGZW010000486         4811 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_863061, whole genome shotgun sequence.
ACCESSION   DBNGZW010000486 DBNGZW010000000
VERSION     DBNGZW010000486.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4811)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4811)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4811
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_863061"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="AC16JC_11350"
     CDS             <1..935
                     /locus_tag="AC16JC_11350"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR5165334.1"
                     /translation="EVTAPAGVSPVPMAARSHWGKKLACGEFVTSVEIVPPRGVDPGP
                     MLAQCRTLKHAGVDAVNVPDGPRAQSRMGVLPPAMLIEREPGLETVIHYCCRDRNLLG
                     MLSDILGAAAAGLRNILIITGDPPKMGPYPDSTAVFDIDSIGLTNLVYRLNHGLDPGN
                     NPLGGGSATRWVIGVGVNPAAVDPDRELSRFHWKVDAGAEFAVTQPVFDPRQLEAFLE
                     RTASYRIPVVAGIWPLVSLRNAEFLANEVPGVSVPEAVLVRMRAAQEKGKDAALAEGV
                     AIAREMFERVRGLVQGVQVSAPFGKVEVALEVLG"
     gene            1304..2899
                     /locus_tag="AC16JC_11355"
     CDS             1304..2899
                     /locus_tag="AC16JC_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR5165335.1"
                     /translation="MRRLLSALAAACVLTVPASAQFFGQPSGFTVENPVLRRLWALGM
                     DSSRASTLAQVLFDSLGPRLTASPGMEAAQDWLVKTYQSWGVDAKKEQYGTWRGWQRG
                     VTHLDLVAPRVRSLEAMMLAWSPGTPKGRPVRAPVIVFPDVADSAAFAAWLPQARGKF
                     VLASFAQPTCRPDTSWEKWGTAATADSMKARRTAALQAWNQRYQHAGFGPTRQALQGM
                     QRRLDAAGVAGILTNTWSQGWGVDKIFDATTQHAPVVDVSCEDYGLLYRLAERGQGPV
                     VELEAQAEARGEVPVFNVVARIPGAELPNEYVMLSAHFDSWDGGSGATDNGTGTVTML
                     EAMRILQLAYPHPRRTILVGHWSSEEQGLNGSSAFVQDHQPIADSLQALFNQDNGTGR
                     IESMSASGFTLAAGNLARYLAQIPAELTRSIKFSFPGSPGGGGTDHASFVTCGAPGFG
                     LGSGDWDYFRYTWHTNRDTYDKISWDDIRNNATLVAMLAYLAAEDPERMPRARRDVFQ
                     PAPNGQPGAWPACRTPLRATPQP"
     gene            2908..3279
                     /locus_tag="AC16JC_11360"
     CDS             2908..3279
                     /locus_tag="AC16JC_11360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015751869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5165336.1"
                     /translation="MTTKPSVKHADDVTAQAVTAGKDTTRQMLIGPDEGPHFALRRFI
                     MQPGGGMPKHTNTVEHEQYVLRGRARIGIGDQVHDVRAGDVVYIPAGTPHWYEAQAGE
                     PFEFLCVVPNLSDTTEILGDR"
     gene            3281..3775
                     /locus_tag="AC16JC_11365"
     CDS             3281..3775
                     /locus_tag="AC16JC_11365"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR5165337.1"
                     /translation="MPAPAHAVQDFYPDDFSHCYGCGRLNAHGLQLKTRWEGEETIAR
                     FRPAPEHMALPGYVYGGLIASLIDCHAMGTAAAATLRAEGRDVGDAPSPRFVTAALRV
                     DYLKPTPLGPELEIRARVRERTPRKVVVESTVSAGGVITVRGEAVAVPLPAAMARPDP
                     GAAP"
     gene            3772..4656
                     /locus_tag="AC16JC_11370"
     CDS             3772..4656
                     /locus_tag="AC16JC_11370"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF025053.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR5165338.1"
                     /translation="MTAPRKSGGIRTRHSGRKPRDDEIDAFGLTHPGKVRRSNQDHFL
                     IASLHKQMEVHVTSLPSPGQLVGGERLAFLAMVADGVGGAPGGEEASRLAAATITRYV
                     TRSLECYYTADSTDEAAFPQALEEAALQCHLDIARHALDDPERAGMATTLTLWLGVWP
                     RAYLLQVGDSRCYILRDGELQQISRDQTMAQELLDQGVITRTDLLGTRWAGVLASAIG
                     GPQAAPVVTRLDQVWGDVGLLCSDGLTRHVGDDRIRERLASMTSARQACESLLEDALD
                     AGGTDNITILVGRSVRKD"
BASE COUNT          740 a         1701 c         1689 g          681 t
ORIGIN      
        1 acgaggtgac cgctccggcc ggcgtctcac cggtcccgat ggccgcgcgg tcccactggg
       61 ggaagaagct cgcgtgcggc gaattcgtga cgagcgtcga gatcgtcccg cccagaggcg
      121 tggacccggg ccccatgctg gcgcagtgcc ggacgctcaa gcacgcgggc gtggacgccg
      181 tcaacgtgcc cgacggcccc cgcgcccaga gtcgcatggg cgtgctcccc ccggcgatgc
      241 tgatcgagcg cgagcctggg ctcgagaccg tgatccacta ctgttgccgc gaccgcaacc
      301 tgctcggcat gctgtccgac atcctgggcg cggcggccgc gggactgcgc aacatcctga
      361 tcatcacggg cgacccgccc aagatgggcc cctaccccga ttcgaccgcc gtcttcgaca
      421 tcgattcgat cgggctcacg aacctcgtct accggctgaa ccacggcctc gaccccggga
      481 acaacccgct cggcggcggc agcgcgaccc gctgggtcat cggcgtcggg gtcaacccgg
      541 ccgccgtgga ccccgaccgg gagctttcgc ggtttcactg gaaggtcgac gccggcgccg
      601 agttcgccgt gacgcagccg gtgttcgacc cgcgccagct cgaggcgttt ctcgaacgca
      661 ccgcctccta ccgcatcccg gtcgtcgcgg ggatctggcc gctcgtctcc ctgcgcaacg
      721 cggagttcct cgcgaacgag gtccccgggg tgtcggtgcc ggaagcggtg ctggtgcgga
      781 tgcgtgcggc gcaggagaaa ggaaaggacg cggcgctggc cgaaggggtg gcgatcgcgc
      841 gggagatgtt cgagcgcgtg cgggggctcg tgcagggggt gcaggtgagc gccccgttcg
      901 ggaaggtgga agtggcgctc gaagtgctgg ggtgacgggc cgagccgcct cttcagggga
      961 cggggccgag gacggtgaca ctggccctcg ttcgccgtgg actgaagtcc cggctcggcc
     1021 cgtgtggcgt cctcagggac gcgtgatcac ggcgcgctcc ggcccggagg tagccttgcg
     1081 aacgtggtcc atgaggcgtg aacgtagagg cgtaccggca cgctgtcctc ctggtcccag
     1141 ggtagggttg ttccagcagg aggacaggct ccgaaatacg cgagccacgc cctaagattg
     1201 caggccggtc cttcaggacc ccaacgggcc gagccggagc ttgagcccac ggaaccaacg
     1261 gtcgatatcg gggacctgcc atactctgaa tgaggagtct cctgtgcgtc gcctgctatc
     1321 cgccctcgcc gccgcctgcg tcctgaccgt ccccgcctcc gcccagttct tcggccagcc
     1381 ctcgggcttc accgtcgaga accccgtgct gcgccgcctc tgggccctcg gcatggacag
     1441 ctcccgggcg agcacgctcg cgcaggtgct gttcgactcg ctgggtccgc ggctcaccgc
     1501 ctcgcctgga atggaagccg cgcaggactg gctcgtgaag acctatcagt cctggggagt
     1561 cgatgcgaag aaggagcagt acggcacctg gcgcggctgg caacgcggcg ttacgcacct
     1621 cgacctcgtg gcaccccgcg tgcgctcgct cgaggcgatg atgctcgcgt ggagccccgg
     1681 gacgcccaag gggcggcccg tccgcgctcc ggtgatcgtg ttccccgatg tggccgacag
     1741 cgccgcgttc gcggcgtggt tgccgcaggc ccggggcaag ttcgtgctgg cgtcgttcgc
     1801 ccagccgacc tgccgcccgg acacgagctg ggagaagtgg ggcaccgcag cgaccgccga
     1861 ctccatgaaa gcacgccgga cggcggccct ccaggcctgg aaccagcgct accagcacgc
     1921 cggcttcggc ccgacgcgcc aggcgctgca ggggatgcaa cggcgcctcg atgccgccgg
     1981 cgtcgcgggc atcctcacga acacctggtc gcagggctgg ggcgtggaca agatcttcga
     2041 tgccacgacg cagcatgccc cggtggtgga cgtctcgtgc gaagactacg ggctgctcta
     2101 ccgcctcgcc gagcgcggcc agggaccggt ggtggagctc gaggcgcagg cggaggcgcg
     2161 cggcgaggtg ccggtgttca acgtcgtcgc ccgcattccc ggcgccgagt tgcccaacga
     2221 gtacgtgatg ctctcggcgc atttcgattc gtgggacggg ggctcgggtg cgaccgacaa
     2281 cggcaccggc acggtcacga tgctcgaagc gatgcgtatc ctgcagctcg cctacccgca
     2341 cccgcgccgc accatcctcg tgggccactg gagcagcgag gagcagggcc tgaacggctc
     2401 gagcgccttc gtccaggacc accagccgat cgccgacagc ctgcaggcgc tgttcaacca
     2461 ggacaacggc acgggccgga tcgagagcat gagcgcgtcc ggattcacat tggccgcggg
     2521 aaatctggcg cgctatctgg cgcagattcc ggcggagctg acgcgcagca tcaagttcag
     2581 cttcccgggc tctccgggcg gcggcggtac cgaccacgcc tccttcgtga cctgcggcgc
     2641 gccaggcttc ggtttgggct ccggggactg ggactacttc cgctacacct ggcacaccaa
     2701 ccgcgacacg tacgacaaga tttcctggga cgacatccgg aacaatgcga cgctggtggc
     2761 catgctcgcc tacctcgccg ccgaagaccc ggagcggatg ccgcgcgcgc gccgcgacgt
     2821 attccagccg gccccgaacg gacagcccgg cgcctggccc gcctgccgca cgccgctgcg
     2881 cgccacccct cagccgtgaa ctcgaccatg acgaccaagc cctcggtgaa gcacgccgac
     2941 gacgtcacgg cccaggccgt gaccgccggc aaggacacga cccgtcagat gctgatcggg
     3001 cccgacgaag gaccgcattt cgcgctgcgc cgcttcatca tgcagccggg cggcggcatg
     3061 ccgaagcaca ccaacaccgt cgagcacgag caatacgtgc tgcgcggccg cgcgcgaatc
     3121 ggcatcggcg accaagtgca cgatgtgcgc gccggcgacg tcgtctacat cccggccggc
     3181 acgccgcact ggtacgaagc ccaggcgggc gagccgttcg agttcctgtg cgtcgttccc
     3241 aatctgtcgg acacgaccga gatcctcggg gatcgctgac gtgccggcgc cggcccacgc
     3301 cgttcaggat ttctatcccg acgacttctc gcactgttac gggtgcggcc gcctcaacgc
     3361 gcacggcctg cagctcaaga cgcgctggga gggcgaggaa acgatcgccc gcttccggcc
     3421 ggcgccggag cacatggcgc ttcccggata cgtgtacggc ggcctgatcg cctcgctgat
     3481 cgattgtcac gcgatgggga cggcggccgc cgcgaccctg cgggccgagg gccgcgacgt
     3541 cggcgacgcc ccgtcccccc gattcgtgac ggccgcgctc cgggtggact acctgaagcc
     3601 cacgccgctc ggtccggagc tcgaaatccg ggcccgcgtg cgcgagcgca cgccgcgcaa
     3661 ggtggtcgtc gagtccacgg tctcggccgg cggagtgatc accgtgcgag gagaagcggt
     3721 cgccgtaccg ctgccggcgg cgatggccag gcccgatcct ggggcagctc cgtgaccgca
     3781 ccgcggaaat cgggggggat ccgcacccgt cactccggtc gcaagccgcg tgacgacgag
     3841 atcgacgcgt tcgggctcac gcatccgggg aaggtccgcc gcagcaacca ggatcacttc
     3901 ctcatcgcct cgctgcacaa gcagatggag gtccacgtga cgagcctgcc cagcccggga
     3961 cagctcgtcg ggggcgagcg cctggcgttc ctggcgatgg tcgccgacgg cgtcggcggt
     4021 gccccgggcg gcgaggaagc cagccggctg gcggccgcga cgatcacccg gtacgtgacc
     4081 cggagtctcg agtgctacta cacggccgac tcgaccgacg aggcggcctt tccgcaggcg
     4141 ctcgaggaag cggcgctcca gtgccatctg gacatcgcgc ggcacgcgct ggacgatccc
     4201 gagcgggccg ggatggccac cacgctcacg ctctggctcg gcgtgtggcc gcgcgcctat
     4261 ctcctgcagg tcggggacag ccgctgctac atcctgcggg acggggagct gcaacagatc
     4321 tcgcgggacc agacgatggc gcaggagctc ctggaccagg gggtcatcac ccggaccgat
     4381 ctcctcggca cgcgctgggc cggcgtgctg gccagcgcga tcggcggccc gcaggcggcg
     4441 ccggtcgtga cgcgactcga tcaggtgtgg ggtgacgtgg ggctgttgtg ctcggacggc
     4501 ctcacccggc acgtcggcga cgatcgcatc cgcgagcgcc tcgcgtcgat gacgtccgcc
     4561 cggcaggcgt gcgagagcct gctcgaggac gccctcgacg cgggcggcac cgacaacatc
     4621 acgatcctgg tcgggcggtc ggtccggaag gactagaacc ccagccagaa gttcatcttc
     4681 aagaggaaga tgttctcggc cggcccccgg aacagcgcgc gcgcgtccgg gccgaagtcg
     4741 atcgtgccgc ggctgagcgc cgactcgctg ctgcgggtcc acaccaggaa cagcgtcgag
     4801 ccgggccggt a
//
LOCUS       DBNGZW010000487         3601 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_590884, whole genome shotgun sequence.
ACCESSION   DBNGZW010000487 DBNGZW010000000
VERSION     DBNGZW010000487.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3601)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3601)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3601
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_590884"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(143..553)
                     /locus_tag="AC16JC_11375"
     CDS             complement(143..553)
                     /locus_tag="AC16JC_11375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR5165339.1"
                     /translation="MPNRLAVGQKARRSLTLTSDHVKRYAELSGDRNPLHFDESFAKS
                     TGKLSGLIVQGGLTTGLLHALVATDLPGPGTVFLSQNWKFTTPVYIGDTITAEAEVLS
                     VHATKPVCQLRVRVSRQTGETVLEGEAWCYRFNA"
     gene            complement(686..1456)
                     /locus_tag="AC16JC_11380"
     CDS             complement(686..1456)
                     /locus_tag="AC16JC_11380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010544195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-disaccharide hydrolase"
                     /protein_id="HIR5165340.1"
                     /translation="MRPPGWLVVPLVLLWVRPVAAQVSTRWPQHSLDRPPTPVVSPAP
                     TTAPGAAPSDAVILFDGRDLSQWVGDSGQAAPWKVENGYVEIAPESGNIHTRQGFGDC
                     QLHVEWMAPLPPHGEGQERGNSGVYLMSRYELQVLDSYDNRTYADGQAASVYGQYPPL
                     ANASRPPGEWQTYDIVFHRPRFDAQGKLLSPARLTVLHNGVLVQDNVELTGPTANQRR
                     PPYTVHPDSLPLLLQNHGNPVRYRNIWIRPLSEQADRQ"
     gene            1556..1777
                     /locus_tag="AC16JC_11385"
     CDS             1556..1777
                     /locus_tag="AC16JC_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165341.1"
                     /translation="MTMRRALQTILTIALFGLVFSGVLTYREFFAGMAKCPSPGAPGT
                     VFGYPACVYGFFMYLVITVVAVLGLRAKR"
     gene            complement(1781..1897)
                     /locus_tag="AC16JC_11390"
     CDS             complement(1781..1897)
                     /locus_tag="AC16JC_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017890415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
                     /protein_id="HIR5165342.1"
                     /translation="MGKGDKRTRRGKIYRGTYGKTRPKKKRRNTRKTAPEGQ"
     gene            complement(1928..2365)
                     /locus_tag="AC16JC_11395"
     CDS             complement(1928..2365)
                     /locus_tag="AC16JC_11395"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5165343.1"
                     /translation="MLASLRSATTPDASCLELFAHVLASEHVWLARLKGAAPHHPVWP
                     ALSLEQCAGLVQANQRDLNAYVAALTPDDLPRGITYTNSAGQTFTTSIEDILLHLCLH
                     GAYHRGQIAWALRRGGGVPLPTDYIALVRGAPAATRDAGAPAR"
     gene            2551..3450
                     /locus_tag="AC16JC_11400"
     CDS             2551..3450
                     /locus_tag="AC16JC_11400"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5165344.1"
                     /translation="MQTSDSPLLVSRLPATAFQADLPSSEFGLAMGFLVGHYFLNMQD
                     LHYGFWPEGLPVEPRNLAQAQAHYTEFLMRHIPAGVRTILDVGCGAGNTARKLLDRGF
                     QVDCVSPNGVLSGVARAVLAGRATIFESRFQDVRTDRRYDLILFSESLLFIPLEEAFA
                     KALALLNPGGHVLITDIFRVPAEGKSPIGGGHELPRFRETIARFPLVPVADEDMTDGI
                     APTFDVLDGAYREAIEPAYRLLLARLTLRHPWVMRVVRWKFRKSMRRYEDKHFSGRRN
                     GANFRKYKSYRLLLYRRGQGSSA"
BASE COUNT          540 a         1194 c         1220 g          647 t
ORIGIN      
        1 cgagggcgcg cagcgcccga ggaatctccg tgtcgaggca cggactacga gatcccgcgc
       61 tgcgctcggg atgacacgcg ctgccctcag atgaccgagg agggcatgct gtcattccga
      121 gggcgcgcag cacccgagga atctaggcgt tgaaacggta gcaccacgcc tccccctcca
      181 gcaccgtttc gccggtctga cgcgacacac gcacccgcaa ctggcatacc ggcttcgtgg
      241 cgtgcacgct cagcacctcc gcttccgccg tgatcgtgtc gccgatgtag accggggtcg
      301 tgaacttcca gttctgactg agaaagaccg ttcccggacc ggggaggtcc gtcgcgacca
      361 gggcgtgcag caggcccgtc gtcaggccgc cctggacgat cagaccggac agtttgcccg
      421 tggacttggc gaaggactcg tcgaagtgca ggggattgcg gtcgcccgac agttcggcgt
      481 accgcttcac gtggtcggaa gtcagcgtga gactgcggcg ggctttctgg cccacggcaa
      541 gacgattcgg cattcgttcc ccagttggca ggtggtcaga cagtcgctca gtcgctcagg
      601 cgcgtagcgg tcgtcagact ccagtcgtca gcagtctttt ctggtgacta atctctgagg
      661 accgctatgc gcctgagtca ctgagtcact gtctgtccgc ctgttcactg agcggcctga
      721 tccagatgtt tctgtacctc accggattcc catgattctg taacagcagt ggcaagctgt
      781 ccgggtgaac ggtgtacggc ggccttctct gatttgccgt tggtcccgtc aactccacgt
      841 tgtcctgcac cagcactcca ttgtgcagca cagtcagacg cgcggggctc agcaatttcc
      901 cctgcgcatc gaagcgcggc cggtgaaaca cgatgtcgta ggtctgccat tcgcccggcg
      961 gcctgctggc gttcgcgagc ggtggatact gtccgtacac ggacgccgcc tgaccgtcgg
     1021 cgtaggtgcg gttgtcgtag gaatcgagga cttgcagttc gtaccggctc atcaggtaca
     1081 ccccgctgtt gccgcgctcc tggccctcgc cgtgcggcgg caggggcgcc atccactcga
     1141 cgtgcagctg gcagtcgccg aagccctgcc gggtgtgaat gttgccgctc tccggggcga
     1201 tctcgacgta cccgttctcg accttccagg gggccgcctg gccggaatcg cccacccact
     1261 gcgagaggtc ccggccatcg aacaggatca cggcgtccga cggcgcggca cccggtgccg
     1321 tggtcggcgc cgggctgacg accggcgtgg gtgggcggtc cagagaatgc tgcggccagc
     1381 gagtggatac ctgggcggcc acggggcgca cccacaggag cacgagcggg acaaccagcc
     1441 agccgggggg tctcatcggg cctccataag aaaccttaca cgttttccgg tgcatcgctt
     1501 cgctccgcgg tcgtagctat agggccggaa cgctggacga ccgggggaga cgaccatgac
     1561 gatgcggcgg gcgctgcaga cgatcctgac gatagccttg ttcggactcg tattctcggg
     1621 ggtgttgacg tatcgcgagt tcttcgccgg catggcgaag tgcccgagcc ccggtgcgcc
     1681 gggcaccgtg ttcggctacc cggcctgcgt gtacgggttc ttcatgtacc tcgtcattac
     1741 cgtagtggcc gtcctgggat tgcgcgccaa gcgctagccc tcactgaccc tcgggcgccg
     1801 ttttgcgggt gttgcggcgt ttcttcttcg gtcgagtctt gccataggtg ccgcggtaga
     1861 tctttccgcg gcgcgtgcgc ttgtcgcctt tgcccatgag taccctcagg tggttggtcg
     1921 ggggaatcta gcgcgccggc gcgccggcgt cccgggtcgc cgcgggtgcg ccgcgcacga
     1981 gggcgatgta atcggtcggc agcggcaccc cgccgccccg tcgcaaggcc caggcgatct
     2041 gtccccggtg ataggcgccg tgcaggcaca ggtgcagcag gatgtcttcg atgctcgtcg
     2101 tgaacgtctg gcccgcgctg ttggtgtacg tgatcccccg cggcagatcg tccggcgtga
     2161 gcgccgccac gtacgcgttg aggtcgcgct ggttcgcctg gacgagtccg gcgcactgct
     2221 cgagcgacag ggccggccag acgggatgat gcggcgccgc tcccttcagc cgggcgagcc
     2281 acacatgttc gctcgccagc acatgggcga agagctcgag acaggacgca tccggggtgg
     2341 tcgcgctccg cagtgatgcg agcacgcggt catccgccca ggcgagatgc gtaacgagct
     2401 tggaaagatg gtgcacggat tcacgtaagc tctccccgcc gtcggccgcg ggcaagcggg
     2461 ggctcgcgtc gcccgccgcc ctctggttat ggttccccgg cgcgcccact ggagggcgcc
     2521 aagccccggg agggacgcac ggccgctgtc atgcaaacct cggattcccc gctgctcgta
     2581 tcgcgcctgc ccgccaccgc cttccaggcg gacctgccgt cgagcgagtt cggcctcgcc
     2641 atggggttcc tggtcggcca ctacttcctc aatatgcagg acctccacta tggattctgg
     2701 cccgaaggcc tgccggtgga gcctcgcaat ctcgcccagg cgcaggcgca ctacacggaa
     2761 ttcctcatgc ggcacatccc cgccggggtg cgcacgattc tcgacgtcgg ctgtggggcg
     2821 gggaacaccg cgcggaagct gctggaccgc gggttccagg tggactgcgt gtcgcccaac
     2881 ggggtgctct cgggcgtggc gcgggcggtg ctggccggac gcgccacgat cttcgaatcg
     2941 cgcttccagg acgtgcggac cgaccgccgc tacgacctga tcctgttcag tgagagcctg
     3001 ctgttcattc cgctggagga ggcgttcgcc aaggcgctgg cgctgctgaa tccgggcggc
     3061 cacgtgctga tcacggacat ctttcgcgtg cccgcggaag ggaagagccc gatcggcggc
     3121 ggccacgagc tgccgcgttt ccgcgagacg atcgcccggt ttccgctcgt tccggtggcc
     3181 gacgaagaca tgaccgacgg catcgcgccg acgttcgacg tgctcgacgg cgcgtaccgc
     3241 gaagcgatcg agcccgccta ccggctgctg ctcgcccggc tcacgctccg gcacccctgg
     3301 gtgatgcggg tggtgcgctg gaaattccgg aagtcgatgc ggcgctacga ggacaagcac
     3361 ttcagcggcc ggcgcaacgg ggccaacttc aggaaataca agtcctatcg cttgctgctt
     3421 tatcgccggg gccagggaag ctctgcgtga gtggcgcgac gccgggggtg ggggagttcg
     3481 gacgtgttgg acgcgggggc gctcgacccg gaccaacgtt tcgcatctca ttgccggcca
     3541 cgagcgacga tgtccaggcc gtgcggcccc cgcgtcctcg cgtcctaccc tccacccccg
     3601 g
//
LOCUS       DBNGZW010000488         8950 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1272084, whole genome shotgun sequence.
ACCESSION   DBNGZW010000488 DBNGZW010000000
VERSION     DBNGZW010000488.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8950)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8950)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8950
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1272084"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1237
                     /locus_tag="AC16JC_11405"
     CDS             <1..1237
                     /locus_tag="AC16JC_11405"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HIR5165345.1"
                     /translation="ARPGGDSRRLEDRFRHLVAASARIYSQHTLPAVLQEVADAARSI
                     VGARYAALALLTPDGSKFESFVTSGIDQATRERIGELPAGRGLLGLVIRERRVIRTAD
                     INYHPQRSGFPPNHPPMHSFLGVPVTTGEKVLGNLYLTEKLDAVEFDDKDEDIAVMLA
                     AQAAVAVENARLSEGSARLLGQIQAMQRQRDLFFAMMNHELRNALTGVYGWAERLVRR
                     KNPESAENAAREVYEGTERTITLLNNFLDLTRLDAGRMQPVRREVDPEAVVRRVLTGF
                     EPAAEAKELTLRAKLPGKVPRFETDPVRLQQILVNLVSNAVRHSPTRGCIEIGVHLNG
                     DGIEFAVSDEGPGIPAEVQERIFEPFERFDPGSGVGTGLGLPVSRRLAEVLGGKLALT
                     SAPGEGASFTLRLPLSLSD"
     gene            1295..1987
                     /locus_tag="AC16JC_11410"
     CDS             1295..1987
                     /locus_tag="AC16JC_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5165346.1"
                     /translation="MKILVVEDDRKVAGFIEQGLREEGYAVDVAPDGDEATMLAHVYD
                     YDLVVLDVMLPKKNGLQVAAELRREGRRTPILMLTARDATEDVVRGLDAGADDYITKP
                     FKFEELLARVRALVRRGGAARTELLVYGPIELDRLKHKVRVQGKPVDLTPKEFHLLQH
                     FLLHPEEVVRRTELLEKVWDMHFDPESNVVDVHVGNLRRKLRQAAGHDLIHTERGVGF
                     RLQQPDESRQPA"
     gene            1984..3384
                     /locus_tag="AC16JC_11415"
     CDS             1984..3384
                     /locus_tag="AC16JC_11415"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF020110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5165347.1"
                     /translation="MRQSLRRTLAVRFAATMAVGLAVMCSVIWWAASRVLRHQLDQSI
                     TASAFLVANELSSSSQCVEGAHALALNAIRYGQTINRYVVFRDPHCAAIRAIPGWAMD
                     LPVDSAALATARGGVQSFSDARWHGMAMRSAYTAVPADGDGEADRVVQVAASLDPVRA
                     LEQVMLVVLLGIVVLGTAATLIGAWSLAGSAVRPVMEITEQATHIEAGTLNQRLSAHA
                     TADEYLGLVGVLNRMLDRLGGAFMAQRRFTADVSHELRTPLTALRGEIEVALRSERAP
                     RDYQRVLHSALEEIDRMTTMTEELLLISRVEAGLIVPQREPVDVDTVVAAALDRLRHR
                     IQDKDLQVEHVTAPSNGCSAQLDRALVRRLVDELLENAVTYTDPGGRIVVRNEHLDDR
                     VRLVVEDNGPGIGAKDLPHLFEPYYRADLARTRGAGTGLGLTSAAAIARLHGGEIRAA
                     NRSEGGARFEVDFPIS"
     gene            3391..5196
                     /locus_tag="AC16JC_11420"
     CDS             3391..5196
                     /locus_tag="AC16JC_11420"
                     /inference="COORDINATES: protein motif:HMM:TIGR01512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HIR5165348.1"
                     /translation="MKAVRRYPIVILAVVGLAVGLSLHVLLPSPDGGHWAFLVVLVGG
                     GMPLVFKTLRGIVRGRLAADIVAALAIVTALVFGEYFAGAVVVLMQSGGEALETYAMH
                     RATSSLEALLARAPKIAHRLTGEQLEDVPVEAVVPGDLLIVRPGDLVPVDGVVTAGTS
                     AVDQSALTGEPIPIRALPGSALMSGSVNMDGALTLRALRKSAESQYQQILRLVDRARA
                     EKPPIVRIADRFAVWFTPVTLVMCGVAYLVTHNPASVLAVLVVATPCPLILAAPVAVI
                     AAISRAAAGGIIVKSGAAIEQVGRARVVVFDKTGTLTLGRPLVQEVSVANGRTPDELL
                     RLAAAVEQLSAHHLGHAVAEAGRRRGLTIPPASDVRETPGLGVSGKVEGRTVDVGSAA
                     YLASRGIPPSDGAAERTTAFVAIDGRYAGFITFADRMRHQVPALMQQLAHLGVDDTVM
                     LTGDREETAEAIAALAGIRTVRANLLPADKVTAVQELKEHRGTVVMVGDGINDAPALA
                     AATVGVAMGEHGPAASAEAADIVLLVDDVSRVGEAIALSQRMRRVILQSVGFGLGVSF
                     ALMVVASTGRIPPSIGAVLQEALDAAVILNALRAR"
     gene            5419..6093
                     /locus_tag="AC16JC_11425"
     CDS             5419..6093
                     /locus_tag="AC16JC_11425"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012701145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR5165349.1"
                     /translation="MHLFPDRSAAGRELAAALLPLRIEDPIVLALPRGGVPVGHEVAH
                     QLGAPLDVLVVRKVGAPDQPELAIGAVGAAGVTVTDEETMALVGVRPEHFLALARAEQ
                     AEVARRDAMFREGQAPLDLRGRNVVLVDDGLATGATMRAAVGVARKLGATQVIVAVPV
                     GAPEACERLAALADRVVCLFTPDAFVAVGYWYADFSQTDDADVQRLLRLARQEREARL
                     ASGVHS"
     gene            complement(6130..8379)
                     /locus_tag="AC16JC_11430"
     CDS             complement(6130..8379)
                     /locus_tag="AC16JC_11430"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5165350.1"
                     /translation="MVTFREHLATALADQYRVDEEIGRGGMATVYLAHDLRPAQQRPV
                     AIKVLHPDLRTALASERFLLEIRVAANLAHPHIVPLHDSGDRDGFLFYVMPYFEGGTL
                     RHRLTRDYQLPVEEAVRIACDVAAALEFAHGKGVVHRDIKPENILFSGETPIVADFGI
                     ARAVTAATIERLTDSQVQIGTPAYMSPEQASGGNIDGRSDIYSLGCVVYEMLTGSVPF
                     TGLTARAILARALVDPVPPIRTVRPAVPPALERAVLKALAKVPADRFATAREFAEALR
                     AHSEGLRDDLSRESVAVLPFDNLSSDPETDYFSDGLSEEIINVLGHISGLRVAARTSS
                     FAFRGKAVDLGEVGARLKVATVVRGSVQRAGHRLRVTVDLSNVADGFLLWSERYDRES
                     HDASDVFAIQDDIAKSVAGRLQLAFGGVERSLLGTPPTRNLDAYNLYLKGRYHWAQRG
                     LGLEKALEFFGQALVLDPDYAAAHAGLADSCLLLAEYGVMPPAAILPKARAAVQRALE
                     LAPDLAEAYCASGELAFVLDWDWARGARDLYRAIELSPGYVTARYRLALYLCLVAGEF
                     VEAIAQARRAVELDPLAPLPWMQLGVVLMGAGRYDEAIAAAQRAIELGPAMLVPYLSL
                     GVLYNHVGRTREAIECLNVAARASGRHPTALTALATCHRALGETGKVQAIYEELSARA
                     RGEFIQKSTLAVAAAAAGQVDEAFRLVGQACEDRDYLLLWAKRHPGFSLLQSDPRMAE
                     VYRRIGFPE"
     gene            complement(8462..>8950)
                     /locus_tag="AC16JC_11435"
     CDS             complement(8462..>8950)
                     /locus_tag="AC16JC_11435"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HIR5165351.1"
                     /translation="VAAVDGVAAAGGFGIAMACDLVMASERASFEWAYHKTGLTGAES
                     STFFLPRLVGLRKAMELVFLNPRLSAKEAKAAGLITAVLPTESFDAEVLALARRLAAG
                     PTRAYAVAKALLNAAAGVDQLDYHLDEELQQLARIADGKDFAEGLDSFFGKRDPVFNG
                     QD"
BASE COUNT         1370 a         3051 c         3150 g         1379 t
ORIGIN      
        1 cgccaggccc ggcggcgaca gccgccgcct cgaggaccgg ttccggcatc tcgtcgcggc
       61 gagcgcccgc atctattccc agcacacact tcccgcggtg cttcaggaag tggccgatgc
      121 cgcccgcagt atcgtcggcg cgcgctatgc ggcgctcgcg ttgctcacgc cggacggctc
      181 caagttcgag agcttcgtca cttcgggcat cgaccaggcg acccgcgagc gcatcggcga
      241 gctgccggcc ggccgcggcc tgctgggact ggtgatccgc gagcgccgcg tgatccggac
      301 ggccgacatc aactaccatc cgcagcggtc cgggttcccg cccaaccacc cgccgatgca
      361 ttcctttctc ggcgtccccg tcacgacggg agagaaggtg ctggggaacc tgtacctcac
      421 cgagaagctg gacgcggtgg aattcgacga caaggacgag gacatcgccg tgatgctggc
      481 cgcgcaggct gccgtcgcgg tggagaacgc ccggttgagc gaggggagcg cgcggctgct
      541 cggacagatc caggccatgc agcgccagcg cgacctgttt ttcgcgatga tgaatcacga
      601 gttgcgcaat gcgctgaccg gcgtgtacgg ctgggccgag cgcctggtcc gcaggaagaa
      661 tcccgagtct gcggaaaacg ctgcccggga agtctacgag ggcaccgaac gcaccatcac
      721 cttgctgaac aactttctcg atctgacccg gctcgacgcc ggccggatgc agccggtgcg
      781 gcgtgaggtg gatccggagg cggtggtgcg gcgggtgctc acgggcttcg agccggctgc
      841 cgaggcgaag gagctcaccc tgagggcgaa gctgccgggc aaggtcccga ggttcgagac
      901 cgacccggtg cgcctgcagc agatcctcgt gaacctcgtg agcaacgccg tgcgtcatag
      961 cccgacgcgt gggtgcatcg agatcggcgt gcacctcaac ggggacggga tcgagtttgc
     1021 ggtcagcgac gaaggcccgg ggatacccgc cgaggttcag gagcgcatct ttgagccgtt
     1081 tgagcgcttc gacccggggt cgggggtcgg gacggggctc ggcctcccgg tctcccggcg
     1141 gctggcggag gtactgggcg gcaagctggc cctgaccagc gcgcccggtg aaggtgcgtc
     1201 cttcacactg aggttgcctc tctcgctctc cgattaaaga tatcttcatg attcccttag
     1261 gaagggttca acgacccgtt cctatgctca tctcatgaaa atcctcgtcg tggaagacga
     1321 ccggaaggtc gcgggcttca tcgagcaggg gctgcgggaa gaaggctacg ccgtggacgt
     1381 ggctcccgac ggcgatgagg cgacgatgct ggctcacgtt tacgactacg atctcgtggt
     1441 cctggacgtg atgctcccca agaagaacgg cctgcaggtg gcggcggagc tgcgccgcga
     1501 gggacggcgc acgccgatcc tcatgctcac ggcgcgggat gccaccgaag acgtagtgcg
     1561 cggcctcgac gcaggggccg acgactacat caccaagccg ttcaagttcg aggagctgct
     1621 ggcccgcgtc cgggcgctgg tgcgccgcgg cggcgccgcc cgcaccgagc tcctcgtcta
     1681 cgggccgatc gagctggacc ggctgaaaca caaggtgcgg gtccagggca agccggtgga
     1741 tctgaccccg aaggagttcc acctgctgca gcatttcctg ctgcatcccg aagaggtcgt
     1801 gcggcgcacc gagctgctcg aaaaggtgtg ggacatgcat ttcgatccgg agagcaacgt
     1861 ggtcgacgtg cacgtgggca acctgcgccg caagctgcgc caggctgccg ggcacgacct
     1921 gatccacacc gagcgcggcg tcgggtttcg ccttcagcag cctgacgaga gccgccaacc
     1981 ggcgtgaggc agtcgctccg gcggacgctc gccgtgcggt tcgcggcgac gatggccgtc
     2041 ggactcgccg tgatgtgcag tgtcatctgg tgggccgcct cccgcgtgct ccgccatcag
     2101 ctggatcaga gcatcaccgc gtcggccttt ctcgtcgcca atgagctgtc ctcttcatcg
     2161 caatgcgtcg agggggcaca cgcgctcgcc ctcaatgcta tccggtacgg tcagacgatc
     2221 aaccgctacg tcgtgttccg cgatccgcac tgcgcggcga tccgcgcgat ccccggctgg
     2281 gccatggacc tcccggtcga cagcgcggcc ctcgcgacgg cgcggggcgg cgtgcagagc
     2341 ttcagcgacg cccggtggca cggcatggcg atgcgttccg cgtacacagc ggttcccgcc
     2401 gacggcgacg gggaggccga ccgcgtggtg caggttgcgg cgtccctcga cccggtgcgg
     2461 gccctggagc aggtgatgct ggtcgtgctg ctcggcatcg tggttcttgg caccgccgcg
     2521 acgttgatcg gcgcgtggag tctcgccgga tcggccgtgc gtcccgtcat ggaaatcacc
     2581 gagcaggcga ctcatatcga ggcgggcacg ctgaaccagc gcctttccgc tcacgctacc
     2641 gctgacgaat acctgggcct ggtcggtgtg ctgaaccgca tgctcgaccg gctgggtggc
     2701 gcgttcatgg cccagcgtcg cttcaccgcc gacgtgagtc acgagctgcg cacgcccctc
     2761 accgcgctcc ggggcgagat cgaggtcgcg ctccgttccg agcgggcgcc ccgcgattac
     2821 cagcgggtgc tgcacagcgc gctggaagag atcgaccgga tgaccacgat gaccgaggag
     2881 ctgctcctga tcagccgcgt cgaagccggg ctcatcgttc cgcaacgcga gccggtcgat
     2941 gtcgacacgg tggtggcggc agcgctcgac cggttgcggc accgcatcca ggacaaggat
     3001 ctgcaggtgg agcacgtgac ggctccctcg aacggctgct cggcgcagct ggatcgcgcc
     3061 ctcgtccgcc gcctcgtgga cgagctgctg gagaacgccg tcacctacac ggatccgggc
     3121 ggccgcatcg tcgtccgcaa cgagcatctg gacgaccggg tgcgcctcgt cgtcgaagac
     3181 aacggccccg ggatcggggc gaaagacctg ccgcacctct tcgagccgta ctatcgcgcc
     3241 gatctggcgc ggacgcgcgg ggccggcacc gggctcggcc tcaccagcgc ggcggcgatc
     3301 gcccggctgc acggcggcga aattcgcgcg gccaaccgct ccgaaggcgg cgcccgcttc
     3361 gaagtcgact ttccgatctc ctgaggccct gtgaaggccg tccggcggta cccgatcgtg
     3421 atactggcgg tcgtcgggtt ggccgtcggg ctctctctcc atgtgctgct tccctcccct
     3481 gacgggggtc actgggcgtt cctggtggtt ctcgtgggcg gcgggatgcc gctggtcttc
     3541 aagacactgc gcggcatcgt ccgcggacgg ctggcggcgg acatcgtggc cgcgctggcg
     3601 atcgtcacgg cgctcgtttt cggcgagtac ttcgccgggg cggtcgtagt gctgatgcaa
     3661 tcgggtgggg aagcgctcga gacctacgcc atgcaccggg ccacgtcgtc gctcgaggcg
     3721 ctgctggccc gcgcccccaa gattgcgcac cggctcaccg gtgagcagct cgaggatgtg
     3781 ccggtcgaag ccgtcgtccc cggcgacctg ctgatcgtgc ggccgggcga cctcgtgccg
     3841 gtcgacggcg tcgtcacggc cgggacgtcc gcggtggacc agtccgcgct caccggtgag
     3901 ccgatcccga tccgggcgct gccgggctcg gccctgatga gcggcagcgt caatatggac
     3961 ggcgccctca cgctccgcgc actgcgcaag agtgccgaga gccagtacca gcagatcctg
     4021 cgcctcgtgg accgggcgcg cgccgagaaa ccgccgatcg tgcgtatcgc cgaccgcttc
     4081 gccgtctggt tcacaccggt gacgctcgtg atgtgcggcg tggcgtatct ggtcacccac
     4141 aatcccgcga gcgtgctcgc ggtcctcgtc gtcgctacgc cctgcccgct catcctggcg
     4201 gcccccgtcg ccgtgatcgc ggccatcagc cgggccgcgg cgggcggcat catcgtgaag
     4261 agcggcgcgg cgatcgagca ggtgggacgg gcgcgggtcg tggtgttcga caagaccggc
     4321 acgctcacgc tcggccggcc gctcgtgcag gaggtgtcgg tcgccaacgg gcgcacgccc
     4381 gacgagctgc tgcggctcgc ggcggcggtc gagcagctct ccgcccatca tctcggccat
     4441 gcggtcgccg aagcggggcg gcgccgcggg ctcaccattc ccccggccag cgacgtccgg
     4501 gagacgcccg gcctgggggt ctcggggaag gtcgaagggc gcaccgtgga cgtcgggtcg
     4561 gcggcgtacc tcgcgagccg cggcattccg ccgagcgacg gcgccgcgga gcgcaccacg
     4621 gcgttcgtgg cgatcgacgg gcgctacgcc gggttcatca ccttcgccga ccggatgcgc
     4681 caccaggtgc ccgccctgat gcagcagctc gcccatctcg gtgtggacga caccgtgatg
     4741 ctcaccggcg accgtgaaga gaccgccgaa gcgatcgcgg cgctggcggg aatccgaacg
     4801 gtgcgggcca atctgctccc cgccgacaag gtgaccgcgg tgcaggagct gaaggagcac
     4861 cgggggaccg tggtcatggt gggcgacggg atcaacgatg cgcccgctct ggccgcggcc
     4921 acggtcggcg tggcgatggg ggagcacggc cccgccgcgt cggccgaggc agccgacatc
     4981 gtgctgctgg tggacgatgt gtcacgggtg ggcgaggcca tcgccctgag ccagcgtatg
     5041 cgccgcgtca tcctgcagag cgtggggttc ggcctcggcg tgagcttcgc cctcatggtg
     5101 gtggcgagta cggggcgaat tcccccgtcc atcggggcgg tgctccagga ggcgctggac
     5161 gcggccgtga tcctgaatgc gctgcgggcc cgctagcctt tcgtgaacca atcttcatga
     5221 cggcatcacg attcccttgg agcaggtggc gcatcttggc gtaggtgacg tagcgctcga
     5281 agcacggagg agggtgccgc caggcatcac acgagaccag gccaacagcc ggtctctggg
     5341 gagtgggcca ctggcccatc cgctccgagg gctacgtcac cggtttgttt ccgggatgga
     5401 ttagggaagg gaggggcggt gcacctgttt cccgaccgca gcgccgcagg ccgtgagctg
     5461 gcggccgctc tgctgccgct ccgcatcgaa gacccgatcg ttctggccct gccgcgcggc
     5521 ggcgtccccg tcggccacga agtggcgcat cagctgggcg caccgctcga cgtgctcgtc
     5581 gtgcgcaagg tgggcgcgcc ggaccagccc gagctggcga tcggcgcggt gggcgcggcg
     5641 ggcgtgacgg tgacggacga ggagaccatg gcgctcgtcg gcgtgagacc ggagcatttc
     5701 ctggccctgg cgcgcgccga gcaggcggag gtggcgcgcc gcgacgcgat gttccgggag
     5761 gggcaagcgc cgctcgacct gcgcggccgc aacgtcgtcc tggtcgacga cggcctggcg
     5821 acgggggcga cgatgcgagc cgcggtaggg gtggcgcgca agctcggggc gacgcaggtc
     5881 atcgtggcgg tccccgtcgg cgcccccgaa gcatgcgagc ggctggccgc gctcgcggac
     5941 cgggtcgtgt gcctgttcac ccccgacgcg ttcgtggcgg tggggtactg gtacgccgac
     6001 ttctcccaga ccgacgacgc cgacgtacag cgactcctgc gactcgcccg gcaggagcga
     6061 gaggcacgtc tcgcctccgg cgttcattcc tgaatggtgc gctcgcggcc gtgctgaagg
     6121 acgcggatct cactccggga atccgatgcg ccgatagacc tccgccattc gagggtccga
     6181 ctgcaacagg ctgaacccgg gatggcgctt cgcccatagc agcaggtagt cgcgatcctc
     6241 gcacgcctga ccgaccaggc gaaacgcttc gtccacctgg cccgcggcgg cggcggccac
     6301 cgccagcgtg gacttctgga tgaactctcc ccgtgcacgc gccgacaatt cctcgtaaat
     6361 ggcctgcacc ttgccggtct ccccaagcgc tcggtgacac gtggccagcg cggtcagcgc
     6421 cgttgggtgc cgtccggacg cccgggcggc gacgttgagg cactcgatcg cctccctggt
     6481 ccgacccacg tgattgtaga ggacgccgag gctcaggtag gggacgagca tggctggacc
     6541 gagctcgatc gcgcgctgtg ccgccgcgat cgcctcgtcg tacctgccgg cccccatcag
     6601 cacgacgccc agctgcatcc atggcaacgg agcaaggggg tcgagctcga cggcacgacg
     6661 ggcctgagcg atggcttcga cgaactctcc cgccacgaga cagaggtaga gagcgagccg
     6721 gtaccgggcc gtgacgtagc cgggactcag ctcgatcgct cgatacaggt cccgggcgcc
     6781 acgtgcccag tcccaatcga gcacgaaggc aagctctccc gacgcgcaat acgcttcggc
     6841 aagatcggga gcgagctcca gtgcccgctg cacggccgca cgtgccttgg gcaggatcgc
     6901 cgcgggaggc atcaccccgt actccgcgag gagcaggcag gaatccgcga gtccggcgtg
     6961 cgccgccgcg tagtccggat cgaggaccag cgcctgaccg aagaattcca gggctttctc
     7021 cagccccagc ccgcgctggg cccagtggta ccgtcccttc aggtacaggt tgtacgcgtc
     7081 cagattcctg gtcggggggg tgccgagcag cgagcgctcg acaccgccga acgccagctg
     7141 gaggcgtccg gccacggatt tggcgatgtc gtcctgaatc gcaaacacgt cgctcgcgtc
     7201 gtgcgactcc ctgtcgtatc gctccgacca caagaggaag ccgtcggcga cgttgctcag
     7261 gtccacggtg acccggaggc gatggccggc gcgttgcacg ctgccccgca ccacggtcgc
     7321 caccttgagc ctcgccccga cctcccccag atccaccgct ttcccccgga acgcgaacga
     7381 cgacgtccgc gcggcgacgc gcagcccgga gatgtggccg aggacgttga tgatctcttc
     7441 actgagacca tcgctgaagt agtcggtctc ggggtcggag ctcagattgt cgaacggcaa
     7501 caccgcgacc gattccctgg agaggtcgtc ccggagtccc tcgctgtgtg cgcggagcgc
     7561 ctcggcgaac tctctggcgg tcgcgaaccg atctgccggc accttcgcga gcgccttcag
     7621 caccgcccgc tccaacgcgg gcggcaccgc cggtcgcacc gtacggatcg gcggcaccgg
     7681 gtcgacgaga gcgcgggcca ggatggcgcg ggcggtcagt ccggtaaacg gaaccgaccc
     7741 cgtgagcatt tcgtagacga cgcagccgag gctgtagatg tcggaccggc cgtcgatgtt
     7801 ccctccggac gcctgctccg ggctcatata cgcgggcgtg ccgatctgca cctgagaatc
     7861 ggtcaggcgc tcgatggtcg ccgcggtcac ggcccgggcg atcccgaagt ccgccacgat
     7921 cggcgtctca cccgagaaca ggatgttttc cggcttgatg tcgcgatgga cgacgccctt
     7981 gccatgtgcg aactcgagcg ccgccgcgac gtcgcaggca atccgcaccg cctcttcgac
     8041 cgggagttgg tagtcgcggg tcaatcggtg gcggagtgtg cctccctcga aatacggcat
     8101 gacgtagaag aggaagccgt cccggtctcc ggaatcgtgc agggggacga tgtggggatg
     8161 cgccagattc gccgcgaccc tgatctccag gaggaaccgc tctgaggcaa gcgcggtgcg
     8221 gaggtccgga tggagcacct tgatggcgac cgggcgctgc tgcgcaggcc ggagatcgtg
     8281 ggcgaggtag accgtggcca tcccaccccg tccgatctct tcatcaaccc ggtactggtc
     8341 cgccagggcg gtggcgaggt gttcccggaa ggtcaccatg ccggggagaa cataggggcg
     8401 aattgtgcgc ttggcgattc ccggggcgag gcatatcccg gtgacgggca acagcgggag
     8461 ttcagtcctg cccgttgaac accggatcgc gcttcccgaa gaacgagtcc aacccctccg
     8521 cgaagtcctt cccgtccgcg atccgcgcca gctgctgcag ctcctcgtcg aggtgatagt
     8581 ccagctggtc cacacccgcg gcggcgttga gcagcgcctt ggccaccgcg taggcccgag
     8641 tcggtccggc cgccagacgc cgcgccaggg ccagcacctc ggcgtcgaag ctctcggtgg
     8701 ggagcaccgc cgtgatcagg ccggccgcct tcgcttcctt ggccgagagc cgcgggttca
     8761 ggaacaccag ctccatcgcc ttgcgcagcc ccacgaggcg gggcaggaag aaggtcgagc
     8821 tctccgcacc cgtcagcccc gtcttgtggt acgcccactc gaacgacgcg cgctccgagg
     8881 ccatgacgag atcgcacgcc atggcgatgc cgaagccgcc ggccgcggcc acgccgtcca
     8941 cggcggcgac
//
LOCUS       DBNGZW010000489        11956 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_524481, whole genome shotgun sequence.
ACCESSION   DBNGZW010000489 DBNGZW010000000
VERSION     DBNGZW010000489.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 11956)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11956)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11956
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_524481"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..525)
                     /locus_tag="AC16JC_11440"
     CDS             complement(<1..525)
                     /locus_tag="AC16JC_11440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165352.1"
                     /translation="MTAGRIIALLAGLATPLAAQSGGARGGDRLTGHVPPATLPALDS
                     IMEDAARTGIPTEPLIQKALEGGAKGVPPDRLVAAVGASAAQLRSASALLRSAGEARP
                     NDPAEVTAVAVALARGVSPQQVARLGAALPGEPTGPALHAVADLVGHGFAEDSSVDLV
                     IAGAQLGLRGLRFLD"
     gene            complement(522..1136)
                     /locus_tag="AC16JC_11445"
     CDS             complement(522..1136)
                     /locus_tag="AC16JC_11445"
                     /inference="COORDINATES: protein motif:HMM:NF027875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen-binding domain-containing protein"
                     /protein_id="HIR5165353.1"
                     /translation="MTPIDPQVHRVLDGELPAETLTETQRAQLEALQRAARTLAAVPQ
                     SSVANRVMAEVRRTPASPAGLGTRLVQWLAERHAVTISFRPAWGLALAAALAAIAIIP
                     SRGPGPALGQQEGIAQFVGRFPGARSVDVVGSFTDWQPGTVVLRDEDHDGIWRGTVVL
                     PVGQHEYMFVVDGERWVTDPLAGRYVDDGFGRVNALLFVRPLTP"
     gene            complement(1133..1702)
                     /locus_tag="AC16JC_11450"
     CDS             complement(1133..1702)
                     /locus_tag="AC16JC_11450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5165354.1"
                     /translation="MNSPRVLSAAERDISDPVVVARVLAGDVEAFGILIGRYEAGLLR
                     YATRMLGSPDAAADAVAEGLVRAYRHLRSCRDPSRLKSWLYRIVGNRCRSHLVRRSAS
                     DVPLEDAPPLLDRADTEAEVERAEQLALVERALQGLSSEKREAFLLKHVEGMSYEEMA
                     EATGERIPTLKMRVHRARAALLEALKEFP"
     gene            complement(1707..3080)
                     /locus_tag="AC16JC_11455"
     CDS             complement(1707..3080)
                     /locus_tag="AC16JC_11455"
                     /inference="COORDINATES: protein motif:HMM:NF015168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HIR5165355.1"
                     /translation="MFALAARLDRPVLLYVGAEDCRWCTVMDREVYSNPELGALIDSL
                     FVPVRVDRDERPDVAERYQAAVLALAGLRGYPLTVFLTPDGAAFFGGTYFPGDDPVTG
                     RGLKQLLPEVARDFRERREILSRHAAVVRQLALARGQGAHGVLRPQALAAEIAAVRIG
                     IDSSVRTGRRFDGLATSQGVMLLLTEYTRTGDSAAFAVARAALATLLDSGAVADASVD
                     QPQEIARAALARVLALAWVFTADTRFRDAGGDQLTRLLRALTYHEGRALFADRDGYAI
                     ATAIATADGLRDSLAERRAIAALDTLLRRVYAPRTGVRHTASGALTGLLQDQVQVADA
                     CIAAYAATGRRRYLDVARDLAAVLDREFADPAGGYYDVAARDAPAAPFDDRVKPVLDD
                     LLPGANAWAAHMLLGLAAATGEADYRRRADATLEAFAGAVEGEGLRAASFLLVARDAL
                     AAGRPRP"
     gene            complement(3090..3239)
                     /locus_tag="AC16JC_11460"
     CDS             complement(3090..3239)
                     /locus_tag="AC16JC_11460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165356.1"
                     /translation="MSARPHSSLLLVPLALACATEHGALTNRMAQTATPYLARAARQP
                     VGWQQ"
     gene            3347..3664
                     /locus_tag="AC16JC_11465"
     CDS             3347..3664
                     /locus_tag="AC16JC_11465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014739939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HIR5165357.1"
                     /translation="MSTAIGVSGGPQAHVAARVLCFPPMTTPVTTITPKVNGPLRVEG
                     PVRILAPDGSEIPVPPRKHGTPAQVVVLCRCGASGTKPFCDGSHKRIGFDSSAGPTLA
                     PPP"
     gene            3786..3962
                     /locus_tag="AC16JC_11470"
     CDS             3786..3962
                     /locus_tag="AC16JC_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015018137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HIR5165358.1"
                     /translation="MAKAGKAPMAADEGYCVKCKTKRQISNAQEVKMANGRPALKGVC
                     PVCGTGMFKILPPK"
     gene            complement(4032..5651)
                     /gene="pruA"
                     /locus_tag="AC16JC_11475"
     CDS             complement(4032..5651)
                     /gene="pruA"
                     /locus_tag="AC16JC_11475"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - L-glutamate gamma-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - L-proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HIR5165359.1"
                     /translation="MSKIPMPLNEPVRAYAPGTPERDELKRALRDMSAQPIEIPLIVG
                     GKEVRTGQTIDAVMPHCHHHVLAKVHQAGSKETAAAVAAARAAWPAWAAASLEERAAV
                     FLKAADLLAGKYRAAVNAATMLGQSKTSHQAEIDAACELIDFFRFNPYFAERIHAEQP
                     VSAPGTWNQLDYRAIEGFVYAISPFNFTSIGGNLCSAPALMGTTVIWKPAATAAYSNY
                     FLMKVMEEAGLPPGVINFLPGPSSQISTALLADRHLGGIHFTGSTEVFQWLWETVGKN
                     LRAYAGYPRLVGETGGKDFILAHASADPDALAAAIVRGAFEYQGQKCSAASRAYVPDS
                     LWPAVRERVLGMLERVRMGDPADFRNFMGAVIDRKAFDKIKEYIDEARTAPGVKVLFG
                     GGADDSDGYFIQPTLLQVDDPKYRTMCEEVFGPVLTLHVYPERRWTETLQLVDATSPY
                     ALTGAVFGRERAAVAEAQRVLRYAAGNFYVNDKPTGAVVGQQPFGGARASGTNDKAGS
                     LFNLIRWVSPRAIKENFSPPGDFAYPFMAEQ"
     gene            complement(5750..7288)
                     /locus_tag="AC16JC_11480"
                     /pseudo
     CDS             complement(5750..7288)
                     /locus_tag="AC16JC_11480"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
     gene            complement(7364..8347)
                     /locus_tag="AC16JC_11485"
     CDS             complement(7364..8347)
                     /locus_tag="AC16JC_11485"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5165360.1"
                     /translation="MDVDLTLKRWGVPVRNGSRYVGCRLSYDGPGVSAPVRMRPAAGP
                     PMGKERRSAERLTLELAGLCLGLSDRRDAPTIGQPPAIAIDPLKDLLTLPAVVAHEAK
                     NPLSGARAGLQLTMESLGRMEDLSVPRRLELLEEMGEVLEALNRALDFLQAVQDRARG
                     AIPGRERFDAVRVARSVVALEGRVLRQVGVEIELATVLETVHLNGDPNLLYELLINLV
                     RNAVEASTERRGPITLRLEREGDTFRISVTDRGIGIPAHLVDRVFEPGFTTKAPGEGG
                     AGLTVVRKVAQEVFGGTVAVKSTEGVGTTFTVSLPIPPQRGPAAPVYSAAT"
     gene            8621..9874
                     /locus_tag="AC16JC_11490"
     CDS             8621..9874
                     /locus_tag="AC16JC_11490"
                     /inference="COORDINATES: protein motif:HMM:NF012355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5165361.1"
                     /translation="MKRRRWQVLCVLGAAAGCGGGGSSGPNTNPAQIVASAGENQVGS
                     AGQQLGTPLEVTVKDASGSPVANVTVTWAAASGGGSVTPASNTTGGDGKATATRTLGP
                     GAGAQTTTATVSGVTPATFHHIAQIQGATQIAANSPVTRSDSVLSTASYSVVVRDQNS
                     NVVAGVIVAWSVTGGGGVLSQLAGTTDVNGITSANLTLDQTAGPRSVQATVTGLQGSP
                     VTFVENGVAGNASVMALNGGNAQVGPVSTALPTPHSVIVHDAHGNPKAGFTVPWVLGL
                     GGGSVSTTTPATDAAGIASVTRTLGAAAGATSDTAKVVGLSGSPVAFTDTAVAVENVA
                     VSNNFFNPATTTISAGTFVKFTWGGGVNHNVTWDGGPVPLPANSVTMSSGTYQARLTL
                     PGTYTYHCTIHGAPGSGMAGSITVN"
     gene            9979..11241
                     /locus_tag="AC16JC_11495"
     CDS             9979..11241
                     /locus_tag="AC16JC_11495"
                     /inference="COORDINATES: protein motif:HMM:NF014428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5165362.1"
                     /translation="MRALRSGLAWLCWAAAACAGGPGPGATPAQIAVSAGNNQIGAAG
                     VPLPLALQVTVRDGAGNPLPGIAVDWIVAGGGSVAPARSITGGDGTATTIRTLGATAG
                     VQATTARVAGSVQVGFTHIARIQGAREIAANGGAAWSDSVRSTVPFTAVVRDQNGAPV
                     AGVVVTWSAARGGVLSQTLDTTDAAGLTAVRFTLSPAAGRQTAQAAVAGLIGSPVSFV
                     DDAVAGNVAQLWVNAGDFQVGPVSANLPLQHSVLVSDAYGNPKASVTVHWAVGDGGGL
                     VNGASAATAATNALGIAAVTRTLGPAAGPHSDSVSVAALPGLPVVFSDTAGALFTIQV
                     GNDFFRPQIDSIPTGSFIKFVWVSGGNLHNITWDPDDPAPPLPNSPNESALNASFIVR
                     ITGVGRYGYHCAYHGGVGTGVFGVIVTE"
     gene            11272..>11956
                     /locus_tag="AC16JC_11500"
     CDS             11272..>11956
                     /locus_tag="AC16JC_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PA domain-containing protein"
                     /protein_id="HIR5165363.1"
                     /translation="MLHALCAIVLLAPFPLQAQQQAPIRGFPSDQVEAQSRREAQARA
                     VPSRDTLRALVRALSAVPHEAGTDRSRHVAELLLARFRAMGLNARIEQFEALMPRPVT
                     RTLELLGPEPFTASLREPALPEDPTSGQADQLPTFNAYSPDGDVTAELVYVNYGRPED
                     YRVLDSLGISVRGKIVIARYGQSWRGIKPKVAAEHGAIGCLIYSDPRDDGYYVDDVYP
                     KGPMRPPTGV"
BASE COUNT         1789 a         4223 c         4197 g         1747 t
ORIGIN      
        1 gtccagaaac cgcaggcccc gcagaccgag ctgcgcgcca gcgatcacca gatccaccga
       61 cgaatcctcc gcgaacccgt gaccgacgag atcggccacg gcgtgcagcg ccggaccggt
      121 gggctcgccc gggagggccg cgcccaggcg ggcgacctgc tggggcgaga ccccgcgagc
      181 cagggcgacg gcgaccgccg tgacctcggc gggatcgttc ggccgtgctt ctccggccga
      241 gcgcagcagg gcgctggcgc tgcgcagctg tgcggcggac gcgccgacgg cggccacgag
      301 gcggtcgggc ggcacgcctt tggcgccacc ctccagcgcc ttctggatga gcggctcggt
      361 cgggataccg gtgcgggccg cgtcctccat gatcgaatcg agcgccggca gcgtggcggg
      421 tgggacgtgc ccggtgaggc ggtcccctcc gcgggcgccg ccactctgag cagccagcgg
      481 cgtggccagc ccggcgagca gcgcgatgat acggcccgcg gtcatggggt gagtggccgg
      541 acgaacagca gcgcgttcac gcggccgaat ccgtcgtcga cgtagcggcc cgccagcggg
      601 tcggtcaccc agcgttcccc gtccacgacg aacatgtact cgtgctgccc gacgggcagg
      661 acgaccgtcc cgcgccagat cccgtcgtgg tcctcatccc gcaggacgac cgtgcccggc
      721 tgccagtcgg tgaacgaacc gaccacgtcg accgatcgcg cgccggggaa gcggccgacg
      781 aactgggcga tcccttcctg ctggccgagc gccggcccgg gaccgcggct cgggatgatg
      841 gcgatggccg cgagggcggc ggccagggca agtccccagg ccggccggaa gctgatcgtc
      901 accgcgtgac gttcggccag ccattgcacc agccgcgtgc ccagacccgc cggggacgcc
      961 ggcgtgcggc gcacctcggc catgacgcgg ttcgcgacgc tcgactgcgg cacggcggcg
     1021 agtgtccgcg ccgcgcgctg cagcgcctcc agctgcgcgc gctgggtctc ggtgagcgtt
     1081 tcggcgggga gctcgccgtc caacacgcgg tgcacctggg gatcgatggg cgtcatggaa
     1141 actccttcag cgcctcgagc agcgctgcgc gggcgcgatg cacccgcatc ttcaacgtcg
     1201 gaatacgctc cccggtcgcc tccgccatct cctcgtagct catgccttcg acgtgcttca
     1261 acaagaacgc ctcacgcttc tccgacgaca ggccctggag ggcccgttcc accagcgcca
     1321 gctgctcggc ccgttcgacc tccgcttcgg tatcggcgcg gtccagcagc ggcggcgcgt
     1381 cctccagcgg tacatcggac gcggagcgcc ggaccaggtg actccggcac cggttcccca
     1441 cgatgcggta cagccagctc ttcaggcgcg atgggtcgcg gcaggagcgc aaatgacggt
     1501 acgcccgtac cagcccttcg gcgacggcat ccgccgccgc atcgggcgac cccagcatcc
     1561 gcgtcgcgta tcgcaacaga cccgcttcgt agcggccgat caggataccg aatgcttcga
     1621 cgtcgccggc gaggacacgc gcgacgacga ccggatctga gatgtcccgt tccgctgccg
     1681 ataagacccg aggagaattc acccggtcac ggtcgcggcc gcccggcggc cagggcgtcc
     1741 cgcgcgacga gcaggaagct cgcggcgcgc aggccctctc cttccacggc gccggcgaac
     1801 gcctcgagcg tcgcatcggc ccggcggcgg tagtccgcct cgccggtcgc cgctgccaga
     1861 ccgagcagca tgtgcgccgc ccacgcgttg gcgccgggga gcaggtcgtc cagcacgggc
     1921 ttcacgcggt cgtcgaacgg cgcggcgggc gcgtcgcgtg cggcgacgtc gtagtagccg
     1981 ccggcggggt cggcgaattc ccggtcgagg acggccgcga ggtcgcgggc gacgtcgagg
     2041 tagcggcgtc gcccggtggc ggcgtatgcc gcgatgcacg cgtcggccac ctgcacctga
     2101 tcctgcagca atccggtgag ggcaccgctc gcggtgtgcc gcacgccggt gcgcggtgcg
     2161 tacacccggc gcagcagcgt gtcgagtgcc gcgatggccc ggcgctcggc cagcgagtcc
     2221 cgcaggccgt ccgcggtcgc gatcgccgtc gcgatggcgt agccgtcacg atcggcgaac
     2281 agcgcgcggc cctcgtggta ggtcagcgcc cgcagcaacc gcgtgagctg atcgccgccg
     2341 gcgtcgcgaa accgcgtgtc ggcggtgaac acccaggcca gcgccaggac gcgcgccagc
     2401 gccgcgcgcg cgatctcctg cggctggtcc accgacgcgt ccgccaccgc gcccgaatcg
     2461 agcaatgtcg cgagcgccgc ccgcgcgacc gcgaacgcgg cactgtcgcc ggtgcgcgtg
     2521 tactcggtga gcagcagcat caccccctgt gaggtcgcca ggccgtcgaa gcgccggccg
     2581 gtccgcaccg acgaatcgat gccgatccgc accgcggcga tctcggccgc cagcgcctgg
     2641 ggccggagca cgccgtgcgc gccctgcccg cgcgccagcg ccagctgccg caccaccgcg
     2701 gcgtgccgcg acaggatctc acggcgctcg cggaagtccc tggcgacctc gggcagcagc
     2761 tgtttcagcc cgcgcccggt gacggggtcg tcgcccggga aatacgtgcc gccgaagaac
     2821 gccgccccgt cgggcgtgag aaacaccgtc agcgggtagc cgcgcaaccc ggcgagcgcg
     2881 agcacggcgg cctgataacg ctccgccacg tccggccgct cgtcacgatc gacgcgcacc
     2941 ggaacgaaca gggagtcgat gagcgcgccc agttcgggat tggagtacac ctcccggtcc
     3001 atcaccgtgc accagcggca gtcctcggcg cccacgtaca gcagcaccgg ccggtcgagc
     3061 cgggcggcca gcgcgaacac gtcacggctc tactgctgcc agcccaccgg ctggcgcgcg
     3121 gcgcgcgcga gatacggtgt cgccgtctgg gccatgcggt tcgtgagggc gccgtgctcg
     3181 gtggcgcagg cgagtgcgag cggcaccagg agcagggagg aatgcggacg ggctgacatg
     3241 gattcccgag agggaatgta cataccctgc gagccgcgta cccgttcccc cgacggatgc
     3301 ccggcaacgg agcgcatcgc taacgcacag cgcatcgcgg gatccggtga gcaccgcgat
     3361 cggcgtgtcc ggcggccctc aagcccacgt cgccgcgcgg gtgttatgct tcccgccgat
     3421 gacgacaccc gtcacgacca tcacgcccaa ggtgaacggc ccgttgcgcg tggaagggcc
     3481 ggtgcgcatt ctcgcgccgg atggctcgga gatcccggtg ccgccccgca agcacgggac
     3541 accggcgcaa gtcgttgtcc tgtgccgctg tggcgcctcg gggacgaagc ccttctgcga
     3601 cgggtcgcac aagcggatcg gattcgacag ctcggccggg cccaccctgg cccccccgcc
     3661 ctgagcgccg gggggtaacc gtgttatagt tggtgccggt cccgggcccc cgccctccgc
     3721 ccgttgcggc tggggtgcgc caggcccaaa tgcagtgtag ccctgcctct ggtggaggag
     3781 agacaatggc aaaggccgga aaggccccga tggcggccga cgaggggtac tgtgtgaagt
     3841 gcaagacgaa gcgccagatt tccaacgcgc aggaagtgaa gatggcgaac ggccgcccgg
     3901 cgttgaaggg ggtgtgcccg gtgtgcggca cgggcatgtt caagatcctg cctccgaagt
     3961 agtcaccgcg tcggttcagc cgattccaca tagcccgaag gcccggcgtc atcgccgggc
     4021 cttcgtcgca gtcactgttc ggccatgaac ggataggcaa agtcgccggg cggcgagaaa
     4081 ttctccttga tggcgcgcgg tgagacccaa cggatcaggt tgaagagact gccggccttg
     4141 tcgttggtgc cgctggcgcg cgccccgccg aagggctgct gccccaccac cgcgccggtc
     4201 ggcttgtcgt tcacgtagaa gttccccgcc gcgtaccgca gcacgcgctg cgcctcggcg
     4261 acggccgccc gctcccggcc gaacaccgct ccggtgaggg cgtagggcga ggtggcatcg
     4321 accagctgca gcgtctccgt ccagcgtcgc tcggggtaga cgtgcagcgt gagcacgggg
     4381 ccgaagacct cctcgcacat cgtgcggtat ttcgggtcat cgacctggag cagcgtcggc
     4441 tggatgaaat agccgtcgct gtcgtcggcg ccgccgccga acagcacttt gacccccgga
     4501 gccgtccgcg cctcgtcgat gtactccttg atcttgtcga acgcctttcg atcgatcacc
     4561 gcacccatga agttgcggaa gtcggccggg tcgcccatgc ggacgcgctc cagcatcccg
     4621 agcacccgct cgcgcaccgc cggccagagc gagtcgggca cgtaggcgcg ggacgccgcc
     4681 gagcacttct gtccctggta ctcgaacgcc ccgcgcacga tcgcggccgc cagcgcatcg
     4741 gggtcggcgc tcgcgtgggc caggatgaag tccttgccgc ccgtttcgcc caccagacgc
     4801 gggtagcccg catacgcgcg caggttcttc cccaccgtct cccacagcca ctggaagacc
     4861 tcggtggaac ccgtgaagtg gatgccgccc agatggcggt cggcgagcag cgcggtgctg
     4921 atctgcgagg atggaccggg gaggaagttg atcacgccgg gcggcaggcc ggcttcctcc
     4981 atcaccttca tgaggaagta attcgaatag gcggcggtgg ccgcgggttt ccagatgacc
     5041 gtcgtcccca tcagcgccgg agcggagcag aggttgccgc cgatcgaggt gaagttgaat
     5101 ggactgatcg cgtagacgaa cccctcgatc gcgcggtagt cgagctggtt ccaggtgccc
     5161 ggcgccgaca ccggctgctc ggcgtgtata cgctcggcga agtagggatt gaagcggaag
     5221 aagtcgatca gctcgcaggc cgcgtcgatc tcggcctgat gcgacgtctt gctctgcccc
     5281 agcatcgtgg cggcgttgac ggccgcgcgg tacttgccgg cgagcaggtc ggcggccttg
     5341 aggaagaccg cggcccgctc ttcgagggac gcggccgccc acgcgggcca cgccgcccgc
     5401 gccgccgcga cggcggcggc cgtttccttc gaccccgcct ggtgcacctt ggcgagcacg
     5461 tggtggtgac aatgcggcat caccgcgtcg atcgtctggc cggtccggac ttccttcccg
     5521 cccacgatca gcgggatctc gatcggctgg gcactcatgt cgcggagcgc gcgcttcagc
     5581 tcgtcgcgct cgggggtgcc gggcgcgtag gcgcgcaccg gctcgttcag gggcatgggg
     5641 atcttggaca tggacggaat ttagccggat ggcgtccgga aaacctgagg ggagcgggga
     5701 gcgatcgccg aaggtgcaac gacgccgacc gctccccgct ccgcgacgct cagcgcgatc
     5761 ctttcaccat caggctatcc gagacccgca ccgccgccat cggctgcacc gaccgcttcg
     5821 ggccgcgcgg gatcatgccg ttcaggttgg ccagctcgaa cgcggtcacg gccatcacgg
     5881 tcgaggcctg gcgcaggtcc cagtcgatcg ccttgtcgta ggtgtccgat tgggagtggt
     5941 gcgtgtggtt gtacccgcgt gtgatctgat tgaagttgaa cccgggcacg ccgtagggca
     6001 ggaaggagag atggtcggtc ccacccttgt tgttgtcggt gacggtgtcg gcgtgcaggt
     6061 cgtgtaccgg gttgagcagc tcgcgtcaca ggtagtagag atcgagacgg ctctgcagtg
     6121 cctggcccgt gatcgccccc acgccgttgt cgagcacgat caccgcctgc atcgaatcgg
     6181 cttccgccgc atgcgcctcg gcgtacttgc gcgacccgat caagccctgc tcttctcccg
     6241 tgaagaggac gaaccggatg gtgcgccgcg gcttcaaccc ggagcgcgcg atgatacgcg
     6301 ccgcctcgag cgtcgccatg gagcccgtgc cgttgtccga ggcaccggtg cccatgtccc
     6361 acgaatcgag gtgcgcgccg acgatcacga cctgccccgg cagctcgctc cccttgatct
     6421 ccgccaccgt gttccactgg gggacggagt cggtggtcat ggtgttcgtc acgctcgctt
     6481 ccacctgcgg caccgtcccg gcggcgatca ggcggtccag catcccgtag tcctcgtggg
     6541 ccaggaccag gtagggaagt ggatagatgc ggttgggcga gccgctcatg ttcagcaggc
     6601 cctgctcctt cccggcatcg gtgagctgtc ccagcgcgcc catccgcttc agaaggaaag
     6661 gaaggtcgtt ctggaactgc gccaggcggg cgcgcccggc cgaatcgagg ccctgacgct
     6721 gggcgaacag gctgcggaaa cgatcacgct gctgctgcga gtcggccgcc gtcatcgggg
     6781 gcccatcgtt gttccagacc ggcgcggccg gggcgagcat cacccacgcg cccttcaccc
     6841 gggccgcgtt cgccgcgagg ctctcgggag tgccggcgtt gatgcgcacc gccgggccgc
     6901 gcagcgcctt cccccccgtc cccggcgacc acgcccagct ggccgccagg aggtcgtgcg
     6961 agcgggggct cacgaggcgc gcccacatcg ggccgcgggt ccaggtcccg ccgaagttcc
     7021 actgctccag atgggcggcc aacccgtagt cctggaaacg gcgcatcgtc cagtcgttcg
     7081 cggcccgcgc cccgggcgag ccggtgagcc gcggaccgat ggcgtcggtg aggtattgca
     7141 ggttctgcat cgcttccgac cgtttcatcc cctcgtcgat gagagcgccc acgccggccg
     7201 tgtcgacctg gagctgctgt ccggccaggg cggagccggt catcgcggcc gcgacgaccg
     7261 cgatgatgga gaatggatag cgtcgcatac ttcgacctct ggtgagaatg ggtggatgga
     7321 ccgggagcgt gcgccggggg ggtgctggct cgggcgcgtc tccctaggtc gccgcgctgt
     7381 acaccggggc ggcgggaccg cgttgcgggg gaatgggcag cgagacggtg aaggtggtcc
     7441 cgacgccttc ggtcgatttc acggcgacgg tgccgccgaa cacttcctgg gcaaccttgc
     7501 gcaccacggt cagtccggct cccccttccc ccggtgcctt ggtcgtgaac cccggctcga
     7561 acacccggtc tacgaggtgg gccggaatcc cgatgccgcg gtcggtgacc gagatccgga
     7621 acgtatcgcc ttcccgctcc agccgcaggg tgatggggcc acgccgctcg gtcgacgcct
     7681 ccaccgcgtt ccggacgagg ttgatcagca gctcgtacag caggttggga tcgccgttca
     7741 ggtggaccgt ctcgagcacc gtggcgagct cgatctccac cccgacctgg cgcagcaccc
     7801 gtccctccag cgctacgacc gaccgggcca cccgcacggc gtcgaaccgc tcacgccccg
     7861 ggatcgcgcc acgggcgcga tcctgcaccg cctgcaggaa gtcgagagcc cggttgagcg
     7921 cctccagcac ttcccccatc tcctccagca gctcgagccg gcgcggcacc gacaggtcct
     7981 ccatgcgtcc cagcgactcc atggtcagct gcagccccgc ccgcgcgccg gagagagggt
     8041 tcttcgcctc gtgcgcgacc accgccggaa gggtgagcag gtccttcagc ggatcgatcg
     8101 cgatggcggg cggctgcccg atggtcggcg catcccggcg gtcggagaga cccaggcaca
     8161 ggccggcgag ctccagcgtc aggcgctcgg cactgcgccg ctccttcccc atgggcggtc
     8221 ccgccgcggg cctcatgcgc accggtgcgc tcaccccggg cccgtcgtag gacaaccggc
     8281 accccacgta gcggctgccg ttgcgcacgg gtacgcccca ccgtttgagg gtgaggtcga
     8341 cgtccatgtt ggtgaagtgg tgacgcgggc tgggatcgct tgacgctcga ggtatcaacg
     8401 atggcccgtc agtgacctcc caaagcgtca ccggcttgcc gtcgagtgct acgctcgctt
     8461 gccggaggat ctccccggca ctacctcgga atgtctccgt cgccacccgg gcgcaccccc
     8521 tggtgtgaat ccgccatggc cgtggaaggc gcacctaaag tagcaactat gggtcgcaaa
     8581 tggtacaatc tcgtttactt cacaaacccg gaggtgcctc gtgaagcgac gtcgttggca
     8641 ggtgctctgt gtcctcgggg cagcggcggg gtgcggcggc ggcggcagct cgggtcccaa
     8701 caccaatccc gcccagatcg tcgcttcagc aggtgagaac caggtcgggt cggccggcca
     8761 gcagctcggc acacccctgg aggtgacggt caaggacgcc tcggggagcc cggtggccaa
     8821 tgtcaccgtc acctgggccg cagcgagcgg cggcggatcg gtgacgcccg cgagcaacac
     8881 caccggaggg gacggcaagg cgaccgccac ccgcacgctc gggccaggag ccggcgcgca
     8941 gacgactacc gccaccgtgt ccggcgtaac cccggccacg ttccatcata tcgcccagat
     9001 ccagggtgcg acgcagatcg cggcgaacag ccccgtgact cgcagcgaca gcgtgctcag
     9061 cacggcgtcc tactcggttg tggtgcggga ccagaacagc aatgtggtcg cgggcgtgat
     9121 cgtcgcctgg tcggttaccg gcggcggcgg ggtcttgagc cagctcgccg gcaccacgga
     9181 cgtaaacggg atcaccagcg ccaacctgac cctcgaccag accgccggtc cccgctcggt
     9241 gcaggcgacg gtgaccggcc tccaggggtc acccgtgacg ttcgtcgaga acggagtggc
     9301 gggcaatgcc agcgtgatgg cgctgaatgg ggggaatgca caggtgggcc cggtcagcac
     9361 ggccctcccg acgccgcatt cggtgatcgt gcatgacgcg cacggcaatc ccaaggcggg
     9421 gttcacggtg ccatgggtgc tggggctggg cggcggctcg gtgtccacga cgacgcccgc
     9481 caccgacgcg gcggggatcg cttcggtcac gcggacattg ggcgcagcgg caggcgccac
     9541 gagcgacacg gcgaaggtcg tcgggttgag tgggtcaccg gtggcattta ccgacaccgc
     9601 cgtggcggtg gagaacgtcg cggtctccaa caacttcttc aacccggcga cgacgacgat
     9661 atcggcaggg acgttcgtga agttcacctg gggcggtgga gtgaaccaca acgtgacctg
     9721 ggacggtgga ccggtcccgc tccctgccaa ttccgtgacg atgtcaagcg gcacgtacca
     9781 ggcgcgcctc acactcccgg gcacatacac ctaccattgc accattcatg gagcacccgg
     9841 ttccggaatg gcgggatcca tcaccgtgaa ctagccgtcc ggactcgacg tctcggacct
     9901 ccgcgtggcg aggcctccct cgccacgcgc gtcgttccgg agcagattcc cgctacgtcc
     9961 ggtgcgggag gtcgtgccat gcgagcgctt cgatcgggtc tggcctggct ctgttgggcg
    10021 gccgccgcgt gcgccggcgg acccggtccg ggtgcgactc ccgcgcagat cgccgtctcg
    10081 gcggggaaca accagatcgg cgcggcgggc gtgccactcc ccctggcgct gcaggtgacg
    10141 gtccgggacg gcgcgggcaa cccgctgccg gggatcgcgg tcgactggat cgtcgcgggt
    10201 ggtggctcgg tggcgccggc gcgcagcatc accggcggcg acggcacggc gaccacgatc
    10261 cgaaccctcg gcgccaccgc cggtgtgcag gcgaccacgg cacgggtggc cggcagcgtg
    10321 caggtcggat tcacccacat cgcccggatc cagggtgcca gggagatcgc cgcgaacggc
    10381 ggcgccgcct ggtccgacag cgtccggagc acggtgccct tcaccgcggt cgtgcgcgat
    10441 cagaacggcg cgccggtcgc cggagttgtc gtgacgtggt cggccgccag gggcggcgtt
    10501 ctcagtcaga ccctggatac gaccgacgcc gcaggtctca ccgcggtgcg cttcactctg
    10561 tctcccgccg ccggccgcca gaccgcgcag gccgcggtgg cggggttgat cggatcgccc
    10621 gtgagcttcg tggacgatgc cgtcgccggg aacgtcgcgc agctgtgggt gaacgccggc
    10681 gatttccagg tcgggccggt atcggccaac ctgccgctgc aacacagcgt cctcgtgagc
    10741 gatgcgtacg gcaacccgaa ggcctcggtg accgtgcact gggccgtggg cgatggcggc
    10801 ggcctggtga atggggcgag cgccgcgacc gccgccacca acgcgctggg catcgccgcg
    10861 gtgacccgca cgctgggacc ggcggccggg ccgcacagcg actctgtgag cgtcgcggcg
    10921 ctgccgggct tgccggtcgt gttctccgat acggccgggg cgctgttcac gattcaggtc
    10981 ggcaacgact tcttccgccc ccagatcgac tcgatcccca cgggcagctt catcaagttc
    11041 gtctgggtgt cgggcggcaa tctccacaac atcacctggg acccggacga cccggcgccg
    11101 cccctcccga actctcccaa cgagagcgcg ctcaacgctt ccttcatcgt gcgcatcacc
    11161 ggggtaggcc gctacggcta ccactgcgcg tatcacggcg gcgtcggcac cggggtgttc
    11221 ggggtgatcg tgacggagta gtttggctcc gactccatcc gaggctcgct cgtgctccat
    11281 gcactctgcg ccatcgtgct cctcgccccg tttccgctgc aggcgcaaca gcaggcgccg
    11341 atccggggat tccccagcga ccaggtcgag gcccagtccc ggcgggaagc ccaggcccgg
    11401 gccgttccca gccgagacac cctgcgggcg ctggtgcggg cgctttcggc ggtgccgcac
    11461 gaggccggga cggatcgctc ccgacacgtg gcggagctgc tgctggcgcg gttccgcgcg
    11521 atggggttga acgcccggat cgagcagttc gaggcgctca tgccgcgtcc ggtgacgagg
    11581 accctggagc tgctgggtcc cgagccgttc accgcgagtc tccgcgagcc ggcgttgccg
    11641 gaggatccca ccagcgggca ggccgatcag ctccccacct tcaacgcgta ctcccccgac
    11701 ggcgacgtca cggcagagct cgtgtacgtg aactacgggc gccccgagga ctaccgggtg
    11761 ctcgacagcc tgggcatcag cgtgaggggg aagatcgtca tcgcccgcta cggccagtcg
    11821 tggcgcggca tcaagccgaa ggtggcggcg gagcacggag cgatcggctg cctcatctat
    11881 tcggatcccc gggacgacgg ctactacgtg gacgacgtgt acccgaaggg accgatgcgc
    11941 cccccgaccg gcgtgc
//
LOCUS       DBNGZW010000490         2162 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1546084, whole genome shotgun sequence.
ACCESSION   DBNGZW010000490 DBNGZW010000000
VERSION     DBNGZW010000490.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2162)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2162)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2162
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1546084"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1110)
                     /locus_tag="AC16JC_11505"
     CDS             complement(<1..1110)
                     /locus_tag="AC16JC_11505"
                     /inference="COORDINATES: protein motif:HMM:NF021778.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR5165364.1"
                     /translation="MNRWLPALAVIVAACRPHAPGQGSPATGRDTRLPTGRLLDPAGV
                     TSAVGQMPLGMVPSPDGRRVLLLLGGWREHGIQVVDRAGRVTQTLPQSAAFVGLTFSA
                     DGRTLYASGGNTDVIYRYRWADDTASLADSMVLAPRAPRAAGTHYPAGLALSQDGRSL
                     YVAENLADSFAVVNPESGTVVQRLATGRYPYGVAVGADGTVFVSNWGASTVSVFAPVG
                     GGRLAPAPAISAGRHPSALVMNRGATRLFVASGSTDRVSVVDARTRRVLATLADPPPA
                     GPDEGTTPDALALSADGTRLFAAEADANAVAVFDLSPSTADVAAATGSDRLAGRIPVG
                     WYPTALLVRGDTLLVANGKGAGTGPNRWLPQPGRRR"
     gene            complement(1118..>2162)
                     /locus_tag="AC16JC_11510"
     CDS             complement(1118..>2162)
                     /locus_tag="AC16JC_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165365.1"
                     /translation="TGDLVARHAPDAMVFQGPQATIRWVGNEDGFAPYPCWNSIAAPD
                     ARTGIATALHGDPSGDVWMPVEVDVSIRRPNWFWSTTNEAHLVSLDDLVEIYYRSVGR
                     GTQLLLNLPPDRTGRIPEADASRAKQFGDEIRRRFGSPLGETGGSGRSMVLAFERPTR
                     VDHVILQEALNRGERVRGWKLEGYAWDHWHPLDEGSAIGHKCIVPVKPDGYTMLRLTV
                     TRSDGEAVLRRFAAYDAGVAPPRTWRDTARLWADDAVGSWSGDTLSLDLSAKAATAGR
                     YRLRLVSESGDPVELRDLALSVAGQPATQLAHYAPGRRDALFLAIPISDSPVILKARL
                     RGAARGTVLLQRW"
BASE COUNT          310 a          779 c          783 g          290 t
ORIGIN      
        1 ccgccggcgg cccggctgcg gcaaccagcg attgggaccc gtgccggccc cctttccgtt
       61 ggccacgagc agcgtgtcgc cgcgaaccag cagcgcggtg ggataccagc ccacgggtat
      121 ccggccggcg agccggtcgg accccgtggc cgccgcgacg tccgcagtcg agggcgacag
      181 gtcgaacacc gccaccgcgt tcgcatccgc ctcggcggcg aacagccgcg tgccgtcggc
      241 ggagagcgcc agcgcgtcgg gcgtcgtgcc ttcgtccggc cccgctggtg gtggatcggc
      301 cagcgtggcg agcacccggc gggtgcgcgc atccaccacg ctgacgcgat cggtgcttcc
      361 cgaagcgacg aacaggcgcg tggcgccgcg gttcatcacg agcgccgaag gatgccgccc
      421 cgcggagatg gccggagccg gcgcgagacg gccgcctccc accggcgcaa acaccgacac
      481 cgtgctcgcg ccccagttgg aaacgaacac cgtaccgtcc gcgcccacgg cgacgccgta
      541 cgggtaacgc cccgtggcca gccgctgcac caccgttccc gactccgggt tcaccacggc
      601 gaaggagtcg gcgaggttct cggcgacgta cagcgaccgc ccgtcctgtg agagcgcgag
      661 tccggcgggg taatgcgttc cggccgcgcg tggcgcgcgc ggcgccagca ccatcgagtc
      721 cgcgagactc gcggtgtcgt cggcccagcg gtaccggtag atcacgtcgg tattgccgcc
      781 ggaggcgtag agcgtccggc catcggccga aaacgtgagg ccgacgaacg cagccgactg
      841 cggcagcgtt tgcgtcacac ggcccgcgcg atcgacgacc tggatcccgt gctcgcgcca
      901 tccgccgagc agcagcagca cgcggcggcc gtcgggcgac ggcaccatac cgagcggcat
      961 ctgtcccacc gccgacgtaa cgccggccgg gtcgagcaac cggcctgtgg gcagtcgggt
     1021 gtcgcggccg gtggccggcg acccctgccc cggcgcatgc ggccggcagg cggcgacgat
     1081 gacggcgagt gccgggagcc atctgttcat ggacggccta ccagcgttgc aggaggacgg
     1141 tgccgcgcgc ggcgccgcgt aatctggcct tcaggatcac gggactgtcg gagatgggaa
     1201 tcgcgagaaa caaagcgtcc cgccggccgg gtgcgtagtg cgcgagctgt gtggcgggtt
     1261 gcccggcgac gctcaacgcc agatcgcgca gctcgacggg gtcgccgctc tcggagacga
     1321 gccgcagccg gtagcggccg gcggtggcgg ccttcgcgct cagatcgaga ctcagggtgt
     1381 ccccgctcca gctgccgacc gcgtcgtctg cccacagccg ggccgtatcc cgccaggtgc
     1441 gggggggcgc gactccggcg tcgtaggcgg cgaagcggcg cagcaccgcc tcgccgtcgc
     1501 tccgggtcac ggtgaggcgc agcatggtgt acccgtcggg tttcaccgga acgatgcact
     1561 tgtgcccgat ggccgatccc tcgtcgaggg gatgccagtg gtcccacgcg tagccttcca
     1621 gcttccagcc gcggacgcgc tcgcctcgat tcaacgcttc ctgcaggatc acgtggtcca
     1681 cgcgagtcgg gcgctcgaag gcgagcacca tgcttcgccc ggacccgccc gtctccccca
     1741 gcggcgagcc gaaccggcgc cggatttcgt cgccgaactg cttcgcccgg ctcgcgtctg
     1801 cttcggggat tcggccggtg cggtcggggg ggaggttcag cagcagctgc gtgccgcgcc
     1861 cgaccgagcg gtagtagatc tcgacgaggt cgtccagcga gacgaggtgc gcctcgttcg
     1921 tcgtgctcca gaaccagttg gggcggcgaa tcgagacgtc gacctcgacg ggcatccaca
     1981 cgtccccgga gggatcgccg tgcagtgccg tggcgatccc ggtgcgcgca tcgggcgccg
     2041 cgatggaatt ccagcacgga tagggcgcga atccgtcctc gttcccgacc cagcggatgg
     2101 tggcctgcgg cccctggaac accatggcgt ccggcgcgtg gcgggccacc aggtctcccg
     2161 tc
//
LOCUS       DBNGZW010000491         5639 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1737603, whole genome shotgun sequence.
ACCESSION   DBNGZW010000491 DBNGZW010000000
VERSION     DBNGZW010000491.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5639)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5639)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5639
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1737603"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="AC16JC_11515"
     CDS             <1..508
                     /locus_tag="AC16JC_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003786820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR5165366.1"
                     /translation="RRQVELGRDVVIVLDSLTRLARSHNTAGRSSGRTMSGGLDANAM
                     AKPKALFGSARAVPDGGSLTVIATALVETESRMDDVIFEEFKGTGNAELRLTREMADR
                     RIYPAVDIAASGTRREELLAPPAVVSARQELRRRLVGLPPTQAMENLVAMLKRSKTNA
                     DVLGIDAA"
     gene            complement(505..1101)
                     /locus_tag="AC16JC_11520"
     CDS             complement(505..1101)
                     /locus_tag="AC16JC_11520"
                     /inference="COORDINATES: protein motif:HMM:NF018184.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HIR5165367.1"
                     /translation="MKLDLYARHKSEYIAPRSPALVTIGAARYLTVAGRGAPNSPAFG
                     AAVGSLYGIAYTIKMAKKAGGRDFTVAKLEGLWWGGRHGRLLIDSPVKSWRWKVMIRV
                     PRFVATKDLAAARRTLAARGRPLGAPVRLETLREGRCVQVLHRGPYRSEHPTIAAMVA
                     FARGRGLRPRGRHHEIYLSDPRRVAPARLRTILRQPVR"
     gene            complement(1118..2683)
                     /locus_tag="AC16JC_11525"
     CDS             complement(1118..2683)
                     /locus_tag="AC16JC_11525"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR5165368.1"
                     /translation="MRRLKVVLPAFALACGGHRTQPAVVPTPAAAPPAPVGIASITAE
                     DVRGRIYRIADDSMRGRATPSPELDKMAGYVAAEFRRFGLRPGGDGGGFLQRYPLEVY
                     ESIPESAAVWATGAAPTRWTLGQDMKFVQGQPVADRVAEGAVLVTGTPQSGGALDSAA
                     VTGKVAVIPIGTSGFAAAMALLPRHPAAIVAVFDVPDAAWLQFPSQTGKVVQNPNEGG
                     GLVIPPILVVRDTSARAWLAKAGVNLDSARAASTGPLSAGVLPGVTIHVALPRRLLQR
                     TTAPNVVGILEGSDPVLKHQYVVFSGHMDHIGTPSDGEGCAPRGADSICNGADDDGTG
                     SVAVLELAEAFASAPVRTKRSLIFLTVSGEERGLWGSAYFTNHPAVPIDSIVADLNSD
                     MVGRSDTLRDSMVAIGREHSDLGTTLDRVAGAHPELRMTPVGDRWPQERLFFRSDHYN
                     FARKGVPILFFTSGLHPDYHQVSDTPDRIDTEKEARFLRLEYYLGETVANAAQKPQWN
                     PDSYQKIVRGSGN"
     gene            complement(2804..4156)
                     /locus_tag="AC16JC_11530"
     CDS             complement(2804..4156)
                     /locus_tag="AC16JC_11530"
                     /inference="COORDINATES: protein motif:HMM:NF025684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HIR5165369.1"
                     /translation="MLATIVLFTALGGRSLPATPVAASHLDRPRVEVWTNRGDDPFHT
                     GEDARVFLRADRDAYVALFRVDTDGRVRVLFPRDPWDDGFVREGREFEVDDGHGYDAF
                     EIDDYPGVGYVFAVASADPFDFDAITRGDHWDYRVIADGRVRGDPYVALTDLAERIVP
                     PGYDDWDYDITPYYVERRYDYPRFLCYDCHSYATFSYWNPYAYSCVRFRIVVFDDPYY
                     YPYRYYGGRRVVFTRPFRPQPRFIFKDRGGVGDDRFVTRERERPVNDNGRRGVRGVDL
                     GGRGSIPVPDVSRRRAGGIGRQNDAPGVPGRPRSTDPSVRDDDRRDESRREVQPRREV
                     PLQRPDRRTQPDRGRPEQGPQSDEDRRRPATERPVPPARQGGQWSDRGQESRGTPREA
                     QPRRPDPEPRSQPERRQAEPRSQPPRASGGERSEPRGREASPSRGKSSDKPELRRRRP
                     "
     gene            4312..5196
                     /locus_tag="AC16JC_11535"
     CDS             4312..5196
                     /locus_tag="AC16JC_11535"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5165370.1"
                     /translation="MTVRERIDLLDPIEVSRLGGIEIIAQGVVEGFLSGLHRSPFRGF
                     SVEFTEHRAYQPGDEPRYLDWRMLARSDRLFVKQFEEETNLRAMILLDASRSMSWRGA
                     PERLTKREYAQRLAAALGLILLRQRDATGLITFDDAVREVVPPRVKSGQWRRLLRGIV
                     ALPDGRGTAAEAALRQVTSLLVRRGLVVLISDLLLDRSLALVALRFLRHRGHQVVVLH
                     LMDPAERALAGPPEVRFRDPESYESLVVRPRELARAYRDAVQREITAWRSACRRHGIA
                     YHDVMTDEPFGRVLRRLA"
     gene            5193..>5639
                     /locus_tag="AC16JC_11540"
     CDS             5193..>5639
                     /locus_tag="AC16JC_11540"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HIR5165371.1"
                     /translation="MIFLHPLVLFGIVAAAIPALLHLFQRRTPPEVEFPPLRYLNEAE
                     RRSARRLRLRHLLLLILRTALIVVLVLAAARPLVPARRAGAAHPPTALALILDNSLSS
                     GAVSGGQVTLERLKAAARSVLDRATPTDRLWLVLADGIIRAGGKEEL"
BASE COUNT          852 a         1914 c         1980 g          893 t
ORIGIN      
        1 ccggcggcag gtcgagctcg ggcgcgacgt cgtgatcgtc cttgactcgc tcacgcgcct
       61 ggcacgctcg cacaacacgg ctggccgctc cagcgggcgc actatgtcgg gtgggctgga
      121 tgcgaacgcg atggccaagc ccaaggcgct gttcgggtcg gcccgggcgg tgcccgacgg
      181 gggatcgctc accgtcatcg caaccgcgct cgtcgaaacc gagagccgga tggacgacgt
      241 gatcttcgag gagttcaagg ggaccggcaa cgcggagctg cgcctcaccc gcgagatggc
      301 ggaccggcgc atctaccccg ccgtggatat cgccgcgagc gggacccggc gggaggagct
      361 gctcgctccc ccggcggtcg tgagcgcacg gcaggagctg cgccgccgcc tggtggggct
      421 gccccccacg caggcgatgg agaacctggt ggccatgctc aagcgctcga agaccaacgc
      481 cgacgtcctc gggatcgacg ccgcctagcg aacgggttgc cggagaatgg tgcggagtct
      541 cgcaggagcg acgcgccgcg ggtcggagag gtagatctcg tgatgccgac cacgtggccg
      601 gagtccccgg ccgcgcgcga acgccaccat cgcggcgatg gtggggtgct ccgaccggta
      661 gggaccgcgg tgcagcacct gcacgcatcg gccctcgcgc agcgtttcca accggaccgg
      721 ggcgccgagg ggacggcccc gcgccgcgag cgtccgccgc gcggccgcga ggtccttggt
      781 ggcgacgaag cgcggcacgc ggatcatcac cttccagcgc caggacttca cgggcgagtc
      841 gatcaacagt ctcccgtgcc gaccgcccca ccacagccct tccagtttcg ccaccgtgaa
      901 atcccggccg cccgccttct tggccatctt gatcgtgtac gcgatgccgt acagcgaccc
      961 gacggcggcg ccgaacgccg gactgttggg ggcaccgcgc cccgcgacgg tgagatatct
     1021 ggcggctccg atcgtcacga gcgccggcga tcggggggcg atgtactcgg acttgtgccg
     1081 cgcgtagagg tcgagcttca ccgtatgcgc cgggcgctca gttcccgctt ccccggacga
     1141 tcttctggta gctgtccgga ttccactgcg gcttctgcgc cgcgttggcc accgtttcgc
     1201 ccaggtagta ctccagccgg aggaagcgcg cttccttttc cgtatcgatc cggtcggggg
     1261 tgtcgctgac ctggtggtag tcagggtgca gtccgctggt gaagaagagg atcgggacgc
     1321 ccttcctcgc gaagttgtag tgatccgagc ggaagaagag ccgttcctgc ggccaccgat
     1381 cacccaccgg cgtcatgcgc agctcgggat gcgcccccgc gacccggtcg agcgtcgtgc
     1441 ccaggtcgga atgctcccgg ccgatcgcga ccatcgagtc gcggagcgtg tccgagcggc
     1501 ccaccatgtc gctgttgaga tccgccacga tgctgtcgat cggcacggca gggtggttgg
     1561 tgaaatacgc gcttccccac agtccccgtt cctccccgct aacggtcagg aagatgagcg
     1621 aacgcttggt ccggaccggc gccgaggcga acgcttccgc gagctccaat actgccaccg
     1681 agcccgtgcc gtcgtcatcg gctccattgc agatggagtc ggcgcccctg ggcgcgcagc
     1741 cctcgccgtc tgagggtgtg ccgatgtgat ccatgtgacc gctgaagacc acatactggt
     1801 gtttcaggac cgggtccgag ccctcgagga tgcccaccac gttcggtgcg gtggtccgct
     1861 gcagcaaccg ccgcggcagc gcgacgtgga tggtcacgcc gggaaggacg ccggcggaca
     1921 agggtccggt gctcgccgcc cgggccgaat cgaggttcac ccccgccttc gcaagccagg
     1981 cgcgcgcgct ggtgtcgcga acgacgagga tgggcgggat cacgagccca cccccctcgt
     2041 tcgggttctg caccaccttc cctgtctgac tcggaaactg gagccaggcg gcgtccggta
     2101 catcgaacac ggcgacaatg gcagcgggat ggcgcgggag cagggccatc gcggccgcga
     2161 acccggaggt cccaatgggg atgacggcga ccttgcccgt caccgcggcg gaatcgagcg
     2221 cgccgcccga ttgcggcgtt ccggtgacga gcaccgctcc ctccgccacc cggtccgcca
     2281 ctggctggcc ctgcacgaac ttcatgtcct ggccgagcgt ccagcgcgtc ggcgccgccc
     2341 cggtcgccca caccgcggcg ctctcgggaa tcgactcata gacctccagg ggatagcgct
     2401 gcaggaagcc gccgccatcg ccgcccggcc tgagcccgaa gcggcggaac tcggcggcga
     2461 cgtatccggc catcttgtcg agctcaggcg acggcgtcgc ccggccccgc atcgagtcgt
     2521 cggcgatgcg gtagatgcgt ccgcgaacgt cctccgcggt gatagaggcg attccgaccg
     2581 gggcgggagg ggccgcggca ggcgtcggga cgacggctgg ctgcgttcta tggccgccgc
     2641 acgcgagtgc gaaggctggc aggacgactt tcaggcgacg catgcacgct cctctggaga
     2701 cagggcgacg gcgggattgg cggacgtccg cagctggaag ctagcgcgcc cgtccgcgtg
     2761 cgactacgga cgggcgcgtc agtgagttgc aggccgcgac gggctacgga cgccggcgtc
     2821 gcaactcggg cttgtccgaa gacttcccgc ggctggggga cgcttcccgc ccgcgtggct
     2881 ccgagcgctc tcctccggaa gcacgcggcg gctgcgaccg gggctcggct tgccgtcgct
     2941 ccggctgcga gcggggttcg ggatccggac gccgcggctg cgcttcgcgc ggcgtcccgc
     3001 ggctctcctg cccccgatca ctccactggc cgccctggcg cgccggcggg acggggcgtt
     3061 ctgtggcggg acggcggcga tcttcatcgg actgaggtcc ctgctccggg cggccgcgat
     3121 cgggctgagt acggcggtcc gggcgctgca acggcacttc gcgtcgcggt tggacttctc
     3181 ggcggctctc gtcacgccga tcgtcgtcac ggacgctcgg atcggtggag cgaggacgcc
     3241 cggggacgcc gggggcgtcg ttctgacgac cgatacctcc cgcccggcgc cggctgacgt
     3301 cggggacggg gattgagccg cgaccaccca agtccactcc ccgcacgccg cggcgtccgt
     3361 tgtcgttcac cgggcgttcc cgctcacggg tcacgaagcg gtcatccccg acgccacccc
     3421 tgtccttgaa gatgaagcgg ggctgtgggc ggaagggccg agtgaagacc acacgcctgc
     3481 ccccgtaata gcgatagggg taataatagg ggtcgtcgaa caccacgatg cggaaacgca
     3541 cgcacgagta agcgtaagga ttccagtagg agaaagtcgc gtagctgtga cagtcatagc
     3601 acaggaaccg tgggtagtcg tagcgcctct ccacgtaata cggtgtgatg tcgtagtccc
     3661 agtcgtcata gcccggcggc acgatgcgct cggccagatc ggtgagggcg acatacgggt
     3721 ctccacgaac acgaccgtcg gcgatcaccc ggtagtccca gtggtcacca cgcgtgatcg
     3781 cgtcgaagtc gaagggatcc gccgaggcga ccgcgaacac gtatccaacg ccgggatagt
     3841 catcgatctc gaacgcgtcg tagccgtggc cgtcatcgac ctcgaattcg cggccctccc
     3901 ggacgaagcc gtcgtcccag gggtcgcgcg gaaagagtac ccggacgcgg ccgtcggtat
     3961 cgacgcggaa gagggcgacg taggcgtcgc gatccgcacg caggaacact cgcgcgtcct
     4021 cgccggtgtg gaatggatcg tcgccgcggt tggtccagac ttccacgcgg gggcgatcga
     4081 gatgggaggc ggcgacagga gtcgccggca gggagcggcc gccgagggcg gtgaacagca
     4141 cgatggtagc tagcatatga cctccaacgg ggcacaagcg cagggcacgt gccgcgcgcg
     4201 acacgtctct aactctttat tccaaagcat gttgagtcat tgccattcga cgggagaccg
     4261 cccgactgtc ctctaccatg gacaggcgag cgactctagc ttccgcgagc catgacggtc
     4321 cgagagcgaa tcgatctgct cgatcccatc gaggtgtcgc ggctcggcgg catcgagatc
     4381 atcgcccagg gtgtcgtgga aggctttctc tccggactgc atcggtcccc gttccgaggg
     4441 ttctccgtcg agttcaccga gcaccgggcc tatcagcccg gggacgagcc gcgctacctc
     4501 gactggcgca tgctcgcccg gtcggaccgc ctgttcgtga agcaattcga ggaagagacg
     4561 aacctccggg cgatgatcct gctggacgcg agccgctcca tgtcgtggcg cggcgcaccg
     4621 gagcgtctga cgaagcggga gtatgcgcag cgactggcgg cggccctcgg gttgatcctg
     4681 ctgcgccagc gggatgccac cgggctcatc accttcgacg atgcggtgcg cgaggtcgtg
     4741 ccgccgcggg tgaagtcagg gcagtggcga cggttgctgc gcggaatcgt ggcgctgccg
     4801 gacggccggg ggacggcggc cgaagcagca ttgcgtcagg tgacgtccct gttggtgcgc
     4861 cgcggcctcg tcgttctgat atccgacctg ttgctggacc ggagccttgc cctggtggcg
     4921 ctgcggttcc tgcggcaccg cgggcatcag gtcgtggtcc tgcacctgat ggatcctgcc
     4981 gaacgcgcgc tcgccggccc tcccgaagtg cggttccgcg acccggagtc atacgagagt
     5041 ctggtcgtgc gtccgcgcga gctggcgcgc gcataccggg acgccgttca acgcgaaatc
     5101 accgcgtggc gctccgcctg ccgccgtcac ggcatcgcgt atcatgacgt gatgaccgac
     5161 gagccgttcg gtcgcgtcct gcgtcgcctg gcgtgatctt cctccatccg ctggtcctct
     5221 tcggtatcgt cgcggcggcg attccggcgc tgctgcacct gtttcaacgc cgcactccac
     5281 ccgaggtcga gttcccaccg ttacgctacc tgaacgaggc cgaacggcgg agtgcgcgac
     5341 ggttacggtt gcggcacctg ctgttgctga tcttgcgcac ggcactgatc gtcgtgctcg
     5401 tgctggcggc cgcgcgcccg ctcgttcccg cgcgtcgcgc cggtgcggcg catcccccga
     5461 ccgcgctggc actgatcctc gacaactcgt tgtcgtccgg tgccgtttcg ggcgggcagg
     5521 tgacgctcga gcggttgaag gccgcggcgc ggtccgtact cgatcgcgcg acgcccaccg
     5581 accggctgtg gctcgtgctc gcggacggga tcattcgcgc gggtgggaaa gaggagctg
//
LOCUS       DBNGZW010000492         3295 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1546159, whole genome shotgun sequence.
ACCESSION   DBNGZW010000492 DBNGZW010000000
VERSION     DBNGZW010000492.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3295)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3295)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3295
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1546159"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..655
                     /locus_tag="AC16JC_11545"
     CDS             <1..655
                     /locus_tag="AC16JC_11545"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5165372.1"
                     /translation="IAFALGRSRAAVAICARTASAVEQAVAELRAAGIDAIGRPCDVS
                     DPAAVAAFAGFVERERGNAAVLVNNAGIGRFSPLEELTLEDWDLVMGVNVRSLYLMTR
                     AFLPGLAQTGAGAIVNIASLAGKNGIEGGTAYCASKHAVLGFSKALMLEVRKRGIRVI
                     AVCPGSVSTPFMDKQSRLRPNRDRVLRAEDVAQAVLGALTLPDRAMLSELDLRPTNP"
     gene            complement(660..1028)
                     /locus_tag="AC16JC_11550"
     CDS             complement(660..1028)
                     /locus_tag="AC16JC_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165373.1"
                     /translation="MTAPTRRAGYHTVPLPLALHLAGHTVTGLFCMFAGGLNLIDPGH
                     ILRLGLPLLALTGFSWGYVFGILMGRKEVLALGFLASIAYAAVGVLKLGSDRPFGLLL
                     LAIGVYGLAVLTIYRNRIVD"
     gene            1083..1598
                     /locus_tag="AC16JC_11555"
     CDS             1083..1598
                     /locus_tag="AC16JC_11555"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5165374.1"
                     /translation="MRVTRTYLELSAAGQFRPAFGDFPDFQLERVPHPSPALYRECYR
                     TVGAAHRWRDRWDWSDEEIRAHLADPSITFHVGTRAGALAGYYELRRVAGDDSVEVAY
                     FGLVPEALGRGLGKHLLSCAVRDAWAVRPAPRRVWLHTSTLDHPNALPNYVARGFVPY
                     KTEQYEVDSVT"
     gene            1595..1984
                     /locus_tag="AC16JC_11560"
     CDS             1595..1984
                     /locus_tag="AC16JC_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020705717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165375.1"
                     /translation="MNFQFHFSRKAKLITAAVVAAPILAFAIYTWSALTYSYSRGYRG
                     PGYVQKFSKKGWICKTWEGELALLPLPGTFTPERFNFTVRDDVIAQYVNATLGKRVEL
                     TYEQHKGIPTTCFGETEYFVTAVRVIP"
     gene            1981..>3295
                     /locus_tag="AC16JC_11565"
     CDS             1981..>3295
                     /locus_tag="AC16JC_11565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR5165376.1"
                     /translation="MTANSFGGRATLAAGERRFEIYRLDALEKRGLPVARLPFSLKIL
                     LENLLRREDGRSVTAADVEKLARWDPKKAPDEEIAFMPARVLLQDFTGVPAVVDLAAM
                     RDAMAQMGGDPKQVNPLLPAELVIDHSVIVDEFGTKTAFHTNAELEFQRNRERYALLR
                     WAQQTFQGFRVVPPDTGIVHQVNLEYLARVVFTTEGTTPQAYPDTLVGTDSHTTMVNG
                     LGVLGWGVGGIEAEAAMLGQPVSMLIPQVVGFRLTGRLAEGATATDLVLTVTEMLRKK
                     GVVGKFVEFYGPGIATLPLADRATIGNMAPEYGATCGIFPVDAETLRYLELTGRAKEQ
                     VALVEAYCKEQGLFHSADTPDAVYTDTLSLDLGSVEPSLAGPRRPQDRVSLAQVGRTF
                     LAALPTLMKPTSPIAAPPAANGGRWESEGGGTAVAVEAPPTRTHVA"
BASE COUNT          542 a         1137 c         1115 g          501 t
ORIGIN      
        1 catcgccttt gcgctgggcc gctcgcgcgc cgccgttgcg atctgcgcgc gaacggcgtc
       61 cgcggtggag caggccgtgg ccgagctgcg tgcggccggc atcgacgcga tcggccgccc
      121 ctgcgacgtg tcggatcccg cggcggtcgc cgcgttcgcc ggcttcgtgg agcgtgagcg
      181 cggcaacgcg gcggtgctcg tgaacaacgc cggcatcggc cgcttcagtc cgctcgagga
      241 gctcaccctg gaagactggg acctggtgat gggggtgaac gtccggtcgc tgtaccttat
      301 gacgcgcgcg tttctccccg gcctggcgca gaccggcgcg ggcgcgatcg tgaacatcgc
      361 ctcgctcgcg ggaaagaacg gcatcgaggg cggtacggcc tattgcgcct cgaagcacgc
      421 ggtgctggga ttctccaagg cgctgatgct cgaggtgcgc aagcgcggca tccgcgtgat
      481 cgcggtgtgc cccggctcgg tgtccacccc cttcatggac aagcaatcgc ggctgcgccc
      541 gaaccgggat cgcgtgctgc gagccgagga tgtggcccag gccgtactcg gcgcgctgac
      601 cttgccggat cgcgcgatgt tgagcgagct ggatctgcgg ccgacgaacc cctgagacgc
      661 tagtcgacga tccggttgcg gtagatggtg agcacggcga ggccgtacac gccgatggcg
      721 aggagcagca gcccgaaggg acggtcgctg ccgagcttga gcacgccgac cgcggcgtac
      781 gcgatgctgg caaggaaacc cagcgccagg acttccttcc gccccatcag gatcccgaat
      841 acgtatcccc agctgaaccc cgtgagcgcc agcaggggaa ggccgaggcg caggatgtga
      901 ccggggtcga tgagattcag cccgcccgcg aacatgcaga acagcccggt gaccgtgtgg
      961 ccggccaggt gcagggccag cgggagaggc acggtgtgat acccggcgcg ccgggtcggt
     1021 gcggtcatgt ccgaattcac gggggttgga gacgtggcta aaataactcg caggccaacc
     1081 tcatgcgagt gacgcgcacc tatctcgagt tgagcgcggc cggtcaattt cgcccggcct
     1141 tcggcgactt cccggatttc cagctggagc gcgtccccca tccctcgccc gccctctatc
     1201 gcgagtgcta ccggacggtc ggggccgcgc atcgctggcg cgaccgctgg gactggagcg
     1261 acgaagagat ccgtgcgcat ctcgccgacc cgagcatcac gttccacgtc ggcacccggg
     1321 ccggcgcgct ggccggctac tacgagctgc gccgggtagc cggcgacgat tcggtggagg
     1381 tcgcctactt cggcctcgtg ccggaggcgc tcggccgcgg gctcggcaag cacctgctgt
     1441 cgtgcgccgt gcgcgacgcc tgggcggtgc gccccgcacc caggcgcgtg tggctgcaca
     1501 ccagcacgct cgatcacccc aacgcactgc cgaattacgt ggcgcgcggc ttcgtccctt
     1561 ataagacgga acagtacgaa gtagattccg tcacatgaat ttccagttcc acttctcccg
     1621 caaagccaag ctcatcaccg ccgccgtcgt cgctgcgccg attctcgcct tcgccatcta
     1681 cacctggtct gcgctgacct acagctactc acggggctac cgcggtccag gctacgtgca
     1741 gaagttctcg aagaagggct ggatctgcaa aacctgggaa ggggagctgg cactgctgcc
     1801 cttgcccggg acgttcaccc ccgagcggtt caatttcacc gtgcgcgacg acgtcatcgc
     1861 ccagtacgtc aacgccacac tcgggaaacg cgtggagctc acgtatgagc agcacaaggg
     1921 catccccacc acctgtttcg gcgaaaccga atacttcgtg accgcagtga gagtgattcc
     1981 atgacggcaa acagtttcgg cggccgcgcc accctcgccg cgggcgagcg ccgcttcgag
     2041 atctatcggc tcgacgccct cgagaagcgc gggctgcccg tggcccggct ccccttctca
     2101 ctcaagatcc tgctcgagaa cctgctgcgg cgcgaagacg gccgcagcgt gaccgcggcc
     2161 gacgtcgaaa aactcgcccg ctgggacccg aagaaggcgc ccgacgagga aatcgcgttc
     2221 atgccggcgc gcgtcctgtt gcaggacttc accggcgtcc ccgcggtcgt ggatctcgcc
     2281 gcgatgcgcg acgccatggc gcagatgggc ggcgacccga agcaggtgaa cccgctgctc
     2341 cccgccgagc tggtaatcga ccactcggtg atcgtggacg agttcggcac gaaaacggcg
     2401 ttccacacga acgccgagct cgagttccag cgcaaccgcg agcgctacgc cctgctgcgc
     2461 tgggcgcagc agaccttcca gggattccgc gtcgtgcccc ccgacacggg catcgtgcat
     2521 caggtgaacc tcgagtacct ggcccgcgtg gtgttcacga ccgaggggac gaccccgcag
     2581 gcataccccg acacgctcgt cggcaccgac tcccacacca cgatggtgaa cggcctcggc
     2641 gtgctcggtt ggggcgtggg cggaatcgaa gcggaagcgg cgatgctcgg ccagccggtg
     2701 tcgatgctga tcccccaggt cgtcggattc cggctcaccg ggaggctcgc cgagggcgcc
     2761 accgcgaccg acctcgtgct cacggtcacc gagatgctgc gcaagaaggg cgtcgtcggg
     2821 aagttcgtgg agttctacgg accggggatc gccacgctgc cgctcgccga ccgcgccacg
     2881 atcggcaaca tggcgccgga gtacggcgcg acctgcggca ttttcccggt ggatgccgag
     2941 acgctgcgct atctggagct caccggacgt gccaaggagc aggtcgcgct ggtcgaggcc
     3001 tactgcaagg agcaggggct gtttcattcg gcggatacac ccgacgcggt gtacaccgac
     3061 accctgtcgc tcgacctcgg cagcgtggag ccgagtctcg ccggaccgcg ccgcccacag
     3121 gaccgcgtga gtctcgccca ggtgggaagg accttcctcg ccgcgttgcc gacgctcatg
     3181 aagccgacct cgcccatcgc cgcgccaccc gccgccaacg gcgggcgctg ggagagcgag
     3241 ggcggcggca cggcggtcgc ggtggaggcg ccgcccacgc gcacgcacgt ggcgt
//
LOCUS       DBNGZW010000493         4732 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1614899, whole genome shotgun sequence.
ACCESSION   DBNGZW010000493 DBNGZW010000000
VERSION     DBNGZW010000493.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4732)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4732)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4732
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1614899"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1964)
                     /gene="lon"
                     /locus_tag="AC16JC_11570"
     CDS             complement(<1..1964)
                     /gene="lon"
                     /locus_tag="AC16JC_11570"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HIR5165377.1"
                     /translation="MERLPVLPLGKLVVYPHVVLPLSISDPRALQLIDDVVQGEKRLL
                     LGVVRHADNQDPQEGPVMHAGLADLYEVGTLGLIVRMMKLMDGTMRVMIQGVERVWLR
                     QPEDDGAYLRAAYEPLTPSGGDDPRVEPLKRNVVAQFSRVIDLAPYLAEELHEVLEGI
                     SDPGKLADFVAANLDLALPARVELLSLGDIPRRLERLAELLAQELQVLEVGSQIQEKV
                     RAKLDEHQREYVLREQLRVIRQELGEEAGDDDVEDLVQKLDAAGLSPEARRIADRELR
                     RLKQMSPQSAEYHVARTYLEVIAALPWERVSPDRLDLKAAREILDRDHYDLAPIKDRI
                     LEHLAVRTLNPDAHGSILCFVGPPGTGKTSLGQSIAEALGRKFVRVSVGGVRDEAEIR
                     GHRRTYVGAIPGRILHALERCETRNPVLMLDEVDKMGADVRGDPTAALLEVLDPAQNA
                     TFVDHYLELQFDLSGVLFIATANSLAQIPDPLLDRMEVLALAGYTPAEKLAIAKRFLL
                     PRQLKETGLVAELATVADGALERLIGEYTREAGVRQLEREIQGVLRKAARQVVEDGRA
                     AVKVGAKNLEEFAGQPKVLNEVAGREPEVGVATGLAWTPAGGDIMFVEAIQMPGRGQI
                     TLTGQLGDVMRESAQAAWSLLRARAPALGIP"
     gene            complement(1966..2427)
                     /locus_tag="AC16JC_11575"
     CDS             complement(1966..2427)
                     /locus_tag="AC16JC_11575"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR5165378.1"
                     /translation="MTESGSPPHRPPRGSGASTVHLADELEVYFNEVAHGKPVGFVAS
                     PKWKPPTDVYETATDIVVHMDVAGVRPEDFTVEYAEGVLTIAGERPARGEGKRHYHAM
                     EVQIGPFERRLRLPVPVDPTSLRATYDQGFLEVQLTKLPPRLSGAHTVKVT"
     gene            complement(2445..4235)
                     /locus_tag="AC16JC_11580"
     CDS             complement(2445..4235)
                     /locus_tag="AC16JC_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015718686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HIR5165379.1"
                     /translation="MTASLDLALVGNGTIGALVDPLGEIVWSCFPRFDGDPAFCTLLQ
                     PQNPKPEFGYFGVELLDAVSHEQEYLANTPVLLTRLQDGLGGAIEIVDCAPRFRQYGR
                     MFCPMTLVRQIRRNAGNPRVRVRLRPAHHAGAQAMSTNTGSNHIRYIGTDLVLRLTTD
                     ASTTAIGEENPFFLEDTVTLLLGPDETVQGAVADVGRRFLEETIAYWREWVRYLGIPF
                     EWQDAVIRAAITLKLNAYEDTGAVIAAVTTSIPEAVDSGRNWDYRYCWLRDGYFVVNA
                     LNRLGATRTMERYLGYIVNVVAAAPEGRLLPVYGIGGRPVPEERSVGSLAGYRGMGPV
                     RFGNEASRQVQHDVYGAAILAATHVFFDRRLQHHGDAVLFARLETLGERAAHLFDQPD
                     AGIWELRNTERVHTFSSVMCWAACDRLARIAAHLGLEERARYWAAHAARIHEAVCRRS
                     WNPKRGAFVGAMDGDSLDASLLRLNEIGFLAADDPRFAGTVHAIERELRHGDFIFRYV
                     EADDFGTPQNAFLVCTFWYINALAELGRREEARALFENLLSCRNRLGLLAEHIDPRTH
                     EQWGNFVQTYSMVGIIGSAVRLSIRWDQAF"
     gene            complement(4232..>4732)
                     /locus_tag="AC16JC_11585"
     CDS             complement(4232..>4732)
                     /locus_tag="AC16JC_11585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014551776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HIR5165380.1"
                     /translation="YATRVRAMAARINARFPDADAPPIVLQVEHQEPVDVYEYYRAAD
                     LCMVSSLHDGMNLVAKEFVSARNDDRGVLILSQFTGAARELPEAIIVNPYDTEECATA
                     LHLALEMPVREQRARMRLMRGLVQEFNVFRWAGRMLIDAAGMRRRRRLTGHAGADRAA
                     ALGAGS"
BASE COUNT          701 a         1656 c         1675 g          700 t
ORIGIN      
        1 ggaatgccca gcgcgggggc gcgggcgcgc agcagactcc aggccgcctg cgccgactcg
       61 cgcatgacgt cgcccagctg gccggtgagc gtgatctgcc cgcggcccgg catctggatc
      121 gcctcgacga acatgatgtc gccgcccgcc ggcgtccagg cgagccccgt ggctacgccg
      181 acctcgggct cgcggcccgc cacctcgttc agcaccttgg gctggccggc gaactcctcc
      241 aggttcttgg cgcccacctt caccgccgcg cgtccgtcct ccaccacctg gcgggccgcc
      301 ttgcgcagca cgccctggat ctcgcgctcc agctgacgca cgccggcctc gcgcgtgtac
      361 tcgccgatca gccgctccag cgcgccgtcg gccacggtcg ccagctcggc caccagcccg
      421 gtttccttga gctgccgcgg cagcaggaac cgcttggcga tcgccagctt ctcggccggc
      481 gtgtagccgg ccagggcgag cacctccatg cggtccagca acggatcggg aatctgggcg
      541 aggctgttgg cggtcgcgat gaagagcacg cccgagaggt cgaactgcag ctcgaggtag
      601 tggtccacga aggtggcgtt ctgcgccggg tcgagcacct cgagcagggc ggccgtggga
      661 tcgccgcgca cgtcggcgcc catcttgtcc acctcgtcca gcatcagcac cggattgcgc
      721 gtctcgcacc gctccagcgc gtggaggatc cgcccgggaa tcgcgccgac gtaggtgcgg
      781 cggtggccgc ggatctccgc ctcgtcgcgc acgccgccca ccgagacgcg gacgaacttg
      841 cgtcccagcg cctcggcgat gctctggccc agcgaggtct tccccgtccc gggagggccg
      901 acgaagcaca ggatgctgcc gtgcgcatcg gggttcagcg tccggacggc gaggtgctcg
      961 agaatgcggt ccttgatcgg cgcgaggtcg taatggtcgc ggtcgaggat ttcgcgcgcc
     1021 gccttgagat cgagccggtc gggcgagacc cgctcccacg gcagcgccgc gatcacctcg
     1081 agataggtgc gcgccacgtg gtactcggcc gactgaggcg acatctgctt gaggcgccgc
     1141 agctcgcgat cggcgatgcg ccgcgcctcg ggcgacaagc cggccgcgtc gagcttctgc
     1201 accaggtcct cgacgtcgtc gtcgcccgcc tcctcgccca gctcctggcg gatcacccgc
     1261 agctgctcgc gcagcacgta ctcgcgctgg tgctcgtcca gcttggcccg caccttctct
     1321 tggatctgac tgccaacctc gagcacctgc agctcctgcg cgagcagctc ggccagccgc
     1381 tcgagacggc gcgggatgtc gcccagcgac agcagctcca cccgcgcggg gagcgcgaga
     1441 tccaggttgg ccgcgacgaa gtcggcgagc ttgccgggat cggagatccc ctccagcacc
     1501 tcgtgcagct cctcggccag gtagggcgcc aggtcgatca cgcggctgaa ctgcgccacc
     1561 acattgcgct tcagcggctc gacgcgcggg tcgtcgccgc ccgacggggt gagcggctcg
     1621 taggcggcgc gcagataggc gccgtcatcc tccggctgcc gcagccacac tcgctccacg
     1681 ccctggatca tgacgcgcat cgtgccgtcc atcagcttca tcatccgcac gatcaggccg
     1741 agcgtcccga cctcgtagag atcggcgagg ccggcgtgca tcaccggccc ctcctgcgga
     1801 tcctggttgt cggcgtgccg cactaccccc agcagcagcc gcttctcccc ctgcaccacg
     1861 tcgtcgatca gctgcagggc gcgcgggtcg ctgatggaga gcggcagcac gacgtgggga
     1921 tacacgacga gcttgccgag cggcaacacg ggaaggcgct ccacgtcagg tcaccttgac
     1981 ggtgtgcgcg ccggacagcc gtggcgggag cttcgtgagc tggacttcga ggaatccctg
     2041 atcgtaggtg gcgcgcaggc tcgtggggtc caccggcacc gggaggcgca gccgccgctc
     2101 gaacgggccg atctggacct ccatggcgtg atagtgacgc ttcccctcac cccgggcggg
     2161 ccgctccccg gcgatggtga gcaccccctc ggcgtattcg acggtgaagt cctccggccg
     2221 cacacccgcg acgtccatgt gaaccacgat gtcggtggcg gtctcgtaca cgtcggtggg
     2281 aggtttccac ttgggagagg cgacgaagcc cacgggcttg ccgtgggcga cctcgttgaa
     2341 atagacctca agctcgtcgg cgagatgcac cgtggacgcc cccgacccgc ggggcggcct
     2401 atggggagga gaacccgatt ccgtcatggg aagaagataa cgctttagaa cgcctggtcc
     2461 caacgtatcg acaggcgaac cgccgagccg atgatcccca ccatgctgta ggtctgcacg
     2521 aaattccccc attgctcgtg cgtccggggg tcgatgtgct cggccaggag gccgagccgg
     2581 ttgcggcagg agaggaggtt ctcgaagagg gcccgggcct cctcacggcg gcccagctcg
     2641 gccagggcat tgatatacca gaaggtacag acgagaaacg cgttctgggg cgtgccgaag
     2701 tcgtccgctt cgacgtagcg gaagatgaag tcgccgtggc gcagctcccg ctcgatggcg
     2761 tggaccgtgc cggcgaagcg cggatcgtcc gccgcgagga acccgatctc gttgagccgc
     2821 agcaggctgg cgtccaggga gtcgccgtcc atcgcgccca cgaaggcccc gcgcttgggg
     2881 ttccatgacc gccggcacac ggcctcgtgg atgcgggcgg cgtgggccgc ccagtagcgg
     2941 gcgcgctcct ccaggccgag atgcgcggcg atccgggcca gccggtcaca cgccgcccag
     3001 cacatcacgc tggagaaggt gtgcacgcgc tccgtgttgc gcagctccca gatgccggcg
     3061 tccggctggt cgaagagatg cgcggcgcgc tcgcccagcg tctccaggcg ggcgaacagc
     3121 acggcgtcgc cgtggtgctg caggcggcga tcgaagaaca cgtgggtcgc cgccaggatc
     3181 gcggcgccgt agacgtcgtg ctggacctgc cgggacgcct cgttgccgaa ccgtacgggg
     3241 cccatgccgc ggtatcccgc gagcgagccg accgagcgct cctccggaac cgggcgccct
     3301 ccgatgccgt acaccggcag gagccgcccc tcgggagcgg cggccacgac gttgacgatg
     3361 tagccgaggt agcgctccat cgtgcgggtg gcgccgaggc ggttcagggc gttgaccacg
     3421 aagtagccgt cgcgcagcca gcagtagcgg tagtcccagt tgcgcccgct gtccaccgcc
     3481 tcggggatgg aggtcgtcac ggcggcgatc accgcgcccg tgtcctcgta ggcgttgagc
     3541 ttcagggtga tggcggcgcg gatcacggcg tcctgccact cgaaggggat gccgaggtag
     3601 cgcacccact cgcgccagta ggcgatcgtc tcctcgagaa agcggcgccc cacgtcggcg
     3661 accgccccct gcaccgtctc gtccggcccg agcagcaggg tgacggtgtc ttccaggaag
     3721 aagggattct cctcgccgat ggcggtggtg gacgcgtcgg tggtgagccg cagcacgaga
     3781 tcggtgccga tgtatcggat gtggttgctg ccggtgttgg tggacatggc ctgcgcgccg
     3841 gcgtggtggg ccgggcgcag ccgcacgcgc acgcgcgggt tgccggcgtt gcgccggatc
     3901 tgccgcacca gggtcatggg gcagaacatg cgcccgtact ggcggaagcg cggcgcgcaa
     3961 tcgacgatct cgatggcgcc gcccagtccg tcctgcaggc gggtgaggag cacgggggtg
     4021 ttggcgaggt actcctgctc gtggctcacg gcgtcgagca gctcgacgcc gaagtagccg
     4081 aactccggct tggggttctg cggctgcagc agcgtgcaga acgcggggtc gccgtcgaac
     4141 cgggggaaac acgaccagac gatctccccg agcggatcga ccagggcccc gatcgtgccg
     4201 ttgccgacga gggcgaggtc gagcgaggcg gtcatgaacc ggcgccgagc gccgccgccc
     4261 ggtcggcccc ggcatgaccg gtcagacggc ggcggcgtcg catccccgcc gcgtcgatca
     4321 gcatccgccc ggcccagcgg aacacgttga actcctgcac caggccgcgc atgagacgca
     4381 tgcgggcgcg ctgctcgcgc accggcatct cgagcgcgag gtgcagcgcc gtcgcgcact
     4441 cctcggtgtc gtagggattg acgatgatcg cctccggcag ctcgcgcgcg gcgccggtga
     4501 actggctgag gatgagcacg ccgcggtcgt cgttgcgggc cgacacgaac tccttggcga
     4561 cgaggttcat gccgtcgtgc aggctcgaca ccatgcacag gtcggccgcg cggtagtact
     4621 cgtacacgtc caccggctcc tggtgctcca cctggagcac gatcggcggc gcatcggcgt
     4681 ccgggaagcg cgcgttgatg cgggcggcca tggcgcgcac gcgggtcgcg ta
//
LOCUS       DBNGZW010000494         2109 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1680072, whole genome shotgun sequence.
ACCESSION   DBNGZW010000494 DBNGZW010000000
VERSION     DBNGZW010000494.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2109)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2109)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2109
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1680072"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..592)
                     /locus_tag="AC16JC_11590"
     CDS             complement(<1..592)
                     /locus_tag="AC16JC_11590"
                     /inference="COORDINATES: protein motif:HMM:NF013557.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MPN domain-containing protein"
                     /protein_id="HIR5165381.1"
                     /translation="MSDSNTPPPFTPPPDTGVSRPAELHAHLQRVLPVAQSVLWHPTS
                     PAVGVRRQPSGVYPVFFDQKAIAAVQVHYETAGRQGMMGFLVGDLFECPTSHVRYVVI
                     DSTIRLNQAVYGDKTLVIVSRLWERIQDELRKTEGHLIGWYHSHPPIGVELAPGDVET
                     HLQYFKRPWHVALVLGTEHEGPVASLFRPRPGETSVS"
     gene            complement(589..1620)
                     /locus_tag="AC16JC_11595"
     CDS             complement(589..1620)
                     /locus_tag="AC16JC_11595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIR5165382.1"
                     /translation="MTAVPIDLRSDTVTRPSEGMRRAMAAAEVCDDVLDGDPTTRRLE
                     RATAELLGMEDALFFPSGTQANGTGIALCTRPGTELVLEANAHLVTYELAGVAALWGV
                     QIRPVETPDGRLTAPLVRRALRPPSPHLPRVTAVAVENTHNAAGGRVLPLEEMAAIVA
                     AAQQAGLAVHLDGARLWNAAAALGVAPARVVRGATTVMVSFSKGLGCPVGSCLAFAGA
                     RRAEAREVRKRFGGGMRQSGILAATGLYALEHHLPELGRDHEHARLLADRLAGLAAVR
                     VIAPETNIVMLDLTREGDDAGHVAPRLAAAGLLLATYGPRRLRAVTHRDVAAADVERA
                     AGILRSVLA"
     gene            complement(1617..>2109)
                     /locus_tag="AC16JC_11600"
     CDS             complement(1617..>2109)
                     /locus_tag="AC16JC_11600"
                     /inference="COORDINATES: protein motif:HMM:NF015587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR5165383.1"
                     /translation="ERRSWQVGKLIDLGFVLLTGTLMLLSALLPALEARTIGALHESG
                     GFLVEWMYRLSLEIVAFGLSVALFLAVFKVLPSRRIGWRTAVVAAVFCALAFELAKRL
                     YALYVANFVTLDRLASNANAGAFFLLIIWIYYTNCVFLIGGEIAEAYDLARMRRTQRV
                     QLG"
BASE COUNT          328 a          766 c          742 g          273 t
ORIGIN      
        1 tggagaccga ggtctcgccc ggccgcggcc ggaacagact cgccaccggc ccttcgtgct
       61 cggtccccaa gaccagcgcc acgtgccacg ggcgcttgaa gtattgcagg tgggtctcca
      121 cgtcgcccgg ggccagctcc acgccgatcg gcggatggct gtggtaccag ccgatcaggt
      181 ggccctcggt cttgcgcagc tcgtcctgaa tccgctccca cagccgcgac acgatcacca
      241 gcgtcttgtc gccgtacacg gcctggttca gccggatcgt cgagtcgatc acgacgtagc
      301 gcacgtgcga ggtgggacac tcgaacagat cgcccaccag gaagcccatc atcccctgcc
      361 ggcccgccgt ttcgtaatgc acctgcacgg ccgcgatggc cttctgatcg aagaacaccg
      421 ggtacacccc gctcggctga cggcgcacgc ccaccgccgg cgacgtcggg tgccacagca
      481 ccgactgcgc cacgggaagg acacgctgca gatgggcgtg cagctcggcg ggacgactca
      541 ccccggtgtc cgggggcgga gtgaacggcg gcggcgtatt gctgtcgctc atgcgagcac
      601 cgatcgcagg atgccggcgg cgcgctcgac gtcggcggcc gcgacgtcgc ggtgcgtcac
      661 cgcccgcagc ctccgcggtc cgtaggtcgc gaggagcaac ccggcggcgg cgagccgcgg
      721 ggcgacgtgc ccggcgtcgt caccttcccg ggtgagatcg agcatgacga tgttggtctc
      781 cggtgcgatg acgcggacgg ctgccagccc ggcgagccgg tcggccagga ggcgcgcatg
      841 ctcgtggtcc cggccgagct cggggagatg gtgctcgagc gcgtacagcc cggtggcggc
      901 gaggatgccg gactggcgca tcccgccccc gaagcgcttg cggacctccc gggcttccgc
      961 ccggcgcgcg ccggcgaacg ccaggcagga cccgacggga catcccaaac ccttagaaaa
     1021 gctcaccata accgtagtcg ctccccgcac gacccgtgcc ggtgcgaccc ccagcgccgc
     1081 ggcggcgttc cagagccggg ccccatcgag gtggacggcc aaccccgcct gctgcgccgc
     1141 cgccacaatg gcggccatct cctccaaagg caagacccgg ccccccgcgg cgttgtgggt
     1201 gttctccacc gccaccgccg tcacccgcgg caggtgcggc gaggggggac ggagtgcccg
     1261 gcgcaccagc ggtgcggtga ggcggccatc cggcgtctcg acggggcgga tctggacccc
     1321 ccacagcgcc gccaccccgg ccagctcgta ggtgaccagg tgcgcgttcg cttccagcac
     1381 cagctcggtt ccgggccggg tgcacagcgc gatgccggtc ccgttcgcct gcgtgccgga
     1441 ggggaagaag agcgcgtcct ccatgccgag cagctcggcg gtggcgcgct ccagccggcg
     1501 cgtggtgggg tcgccgtcca gcacatcgtc gcacacctcg gccgcggcca tggcccggcg
     1561 catcccctcg gacgggcggg tgaccgtgtc gctgcgcagg tcgatcggca cggccgtcat
     1621 cccagctgca cccgctgcgt gcgccgcatg cgcgccagat cgtacgcctc ggcgatctcg
     1681 ccgccgatga ggaacacgca gttcgtatag tagatccaga tgatcaggag gaagaacgcg
     1741 cccgcgttgg cgttggacgc cagccggtcg agcgtgacga agttggccac gtacagggcg
     1801 tagagccgct tggccagctc gaaggcgagc gcgcagaaca ccgcggcgac caccgcggtg
     1861 cgccagccga tgcggcggct gggcagcacc ttgaagaccg cgaggaacag cgcgacgctg
     1921 aggccgaagg cgacgatctc gaggctcagc cggtacatcc actcgaccag gaaaccgccg
     1981 ctctcgtgga gcgcgccgat ggtgcgcgct tcgagcgcgg gcagcagcgc cgacagcagc
     2041 atgagcgtgc cggtgagcag cacgaacccg aggtcgatga gcttgccgac ctgccaggag
     2101 cgccgctcc
//
LOCUS       DBNGZW010000495         7742 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1737821, whole genome shotgun sequence.
ACCESSION   DBNGZW010000495 DBNGZW010000000
VERSION     DBNGZW010000495.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7742)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7742)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7742
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1737821"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1321
                     /gene="fabF"
                     /locus_tag="AC16JC_11605"
     CDS             77..1321
                     /gene="fabF"
                     /locus_tag="AC16JC_11605"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013823342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HIR5165384.1"
                     /translation="MKRRVAVTGLGVVSPLGTTVAATWEAMVAGKSGAGPITRFDPAA
                     LSVKFACEVKGFDPGLYLDKKEARRYDLFTQFAMGAAVQAVNDACLDKSWDGIDLHRV
                     GVIIGTGTGGIATFEENMRAYLEKGPGRVSPFFVPMFMANVAAAVVSMRYGAKGPNYC
                     TVSACATSGHSLGDAFRLIQHNEADVMIAGGSEAAITPLALASFANMKALSERNDDPR
                     TASRPFDKDRDGFVMGDGAAVVILEEWEHARRRGAKIYAEMVGYGMTADAYHITAPSP
                     DGEGAQRAMRLAMEDGGIRPEQVGYINAHGTSTPHCDAAETAAVKGVFGAQATRLVFG
                     STKSMTGHLLGAAGALEFAVCTLALHEGVIPPTINQFTPDPACDLDSAPNQAVTRQVE
                     VALSNSFGFGGHNVTLAVRRAP"
     gene            1318..2577
                     /gene="mqnE"
                     /locus_tag="AC16JC_11610"
     CDS             1318..2577
                     /gene="mqnE"
                     /locus_tag="AC16JC_11610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HIR5165385.1"
                     /translation="MTAGARDPALAPIVEKVERGERLTRADALALFASHDLLTIGELA
                     DRANRRRNGDRVFFAANQHLNPTNVCILRNTCVFCSFARMPNEEGSYTRTLDEVFAEA
                     DAARDNPTREFHIVGGLHPKLSLEYYLDMFRGLKTRHPDVQIKALTAVEVAHLARIEG
                     MTTRDVLLAMKDAGVSSLPGGGAEVFSPAARATIADRKLTGEEWLAVHREAHRLGIPS
                     NCTMLYGHVETLEDRVDHLLALRALQDETNGFLTYIPLAYHPDHNELGEALGRTGTAT
                     TGCDDLKNIAVGRLVLDNIPHVKTHWPMVTPYISQVSLSFGCDDLEGTVVFERVYHEA
                     GAGTPMWLSYDEIIGLIRGAGKTPVERDSLYQTVRTFEDWVPGTAAKPRADGPEGPTF
                     HIEHRVTARGKGKRQLPVVAGVVEGLE"
     gene            2660..3481
                     /locus_tag="AC16JC_11615"
     CDS             2660..3481
                     /locus_tag="AC16JC_11615"
                     /EC_number="4.2.1.151"
                     /inference="COORDINATES: protein motif:HMM:NF014658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthetic enzyme MqnA/MqnD family
                     protein"
                     /protein_id="HIR5165386.1"
                     /translation="MRLGRIPYINCYPVYGAIDRGLVGVPADLVTGTPAELNDLLVAG
                     ELDVSVISAVAYARHAKDLVLLPELAISCDGPVQSVILFSRRPAEKLDGRAVLRSASS
                     RTSVALLELLCRDVWKIRPRFAEARAEAADLEQLAGLPHDAVLVIGDPALLLAARHVY
                     PYRYDLGEEWKRWTGLPFVFAVWAARRGADAQAVRRIHRALLESRSWGLAHQDELARA
                     ASAATQVPEPECRAYLGGLDYALTYKHLAGLTDFFRRLAAAGLVPDGSLQFLQVA"
     gene            3483..4535
                     /gene="mqnC"
                     /locus_tag="AC16JC_11620"
     CDS             3483..4535
                     /gene="mqnC"
                     /locus_tag="AC16JC_11620"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012283008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HIR5165387.1"
                     /translation="MPDVIDRTSAEFRSHLELYQRAPLLELGRLADDARWRLHPEPVV
                     TYIIDRNINYTNVCVADCKFCAFYRRPKHLEGYVLSFEEIGRKIEEVKALGGVQILMQ
                     GGHNPYIPFEWYLDLLRYIKRNHPIHIHAFSPSEVDFFAGRFGMSIEEVLKELVGAGL
                     DSIPGGGGEILVQDVRDRVAKKKAGADRWLEVMEIAHRQGLKTSVTMMYGLGESDADR
                     IEHLHRVRAVQARTGGFTAFICWPLQPENTELEYLGKTDAVTYLRTQAIARVVLGNVP
                     NIQASWVTMGPKIGQVALRFGANDFGSLMMEENVVSAAGTTWRMTLPEMQRLIGDAGY
                     TPVKRRQDYTLLSEAA"
     gene            4568..4963
                     /gene="purE"
                     /locus_tag="AC16JC_11625"
     CDS             4568..4963
                     /gene="purE"
                     /locus_tag="AC16JC_11625"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HIR5165388.1"
                     /translation="MPDVLILVGSESDKPRIEPAFEVLDKAGVSYEFHVSSAHRTPDR
                     TGELVKGAKARGVRVLICGAGLSAALPGFAAALTDLPVIGVPFAVGPLNGLDALLATV
                     QVPPGVPVATVGIDNAKNAAHLALRVLQR"
     gene            4966..5445
                     /gene="ispF"
                     /locus_tag="AC16JC_11630"
     CDS             4966..5445
                     /gene="ispF"
                     /locus_tag="AC16JC_11630"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR5165389.1"
                     /translation="MRIGIGYDSHRFAEGRALVLGGVSVPHHVGLDGWSDADAVAHAL
                     IDAILGAAGQGDIGQMFPSDDPKWKDAGSLDLLRRASLKVAELGLSLAHADITIIAEQ
                     PRLAPYVDQMREKLADAAVVGFSNLSVKAKTNEGMGFIGRGEGVAVIAVALLEEART"
     gene            5562..6044
                     /locus_tag="AC16JC_11635"
     CDS             5562..6044
                     /locus_tag="AC16JC_11635"
                     /inference="COORDINATES: protein motif:HMM:NF020893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HIR5165390.1"
                     /translation="MGAFLAGRGTLDPWIVFAVTWVGNVGSASLVYAVGRRYGRAFFT
                     GRIGRRLLSESTLAHIEEAYARHGTWGIFVSRLLPVWRAVVPPFAGVARVPAARAILS
                     MAVASALYYGTLTFLVATLGTNFDLVTRVLARANLVLGVVAVVLILLFVRWSYRRLKQ
                     "
     gene            6017..6940
                     /gene="xerD"
                     /locus_tag="AC16JC_11640"
     CDS             6017..6940
                     /gene="xerD"
                     /locus_tag="AC16JC_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HIR5165391.1"
                     /translation="MELPAAETVDIPRAFRLEQFRDYLALEVGSSRHTVESYLRDVRR
                     LATHAAGLGIDQPDRLTPVQLREFVYALKDLGLSPATIHRQISAIRGYFRFLVGEGHA
                     ARNPSERLESPKRWRTLPTVLTAHEIDRLLTAPTPDTPLGIRDRALLEFAYATGARVS
                     EVVGVSLQDVLYEEGLARLFGKGSKQRLVPVGRRALGAVALYAREIRPRLDRGKTRGV
                     LFLNARGTPLSRVGAWGVIRQAAKRAGIAKRVSPHTLRHTFATHLLEGGADLRAVQEM
                     LGHVDLATTQLYTHVDRDYLRSVHRQFHPRA"
     gene            7016..7519
                     /locus_tag="AC16JC_11645"
     CDS             7016..7519
                     /locus_tag="AC16JC_11645"
                     /inference="COORDINATES: protein motif:HMM:NF019241.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5165392.1"
                     /translation="MLSDYLVGVIVRDMKALGREVEAYPDDASLWRTPPGIANSGGTL
                     ALHLAGNVQHFFGAVLGGTGYVRDRNAEFSRRDVPRSEIAAQIQAAVAAAERGLAQAS
                     DAVMARDYPEQIAGHTIVTGEWLVHLVAHLGYHLGQVDYHRRLVTGNGETVKVMAAPE
                     LRTARKQ"
     gene            complement(7551..>7742)
                     /locus_tag="AC16JC_11650"
     CDS             complement(7551..>7742)
                     /locus_tag="AC16JC_11650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165393.1"
                     /translation="LSRLPWDRGQWVEDVKFTWFCRDADGTLRRLRVEIRDIGLKPDW
                     KYYTPLGLVRIESVALSSP"
BASE COUNT         1176 a         2790 c         2532 g         1244 t
ORIGIN      
        1 gcggagaagc tccgcaccgt cggcgacgcc atgaaatacc tgcacgacaa gatggggaag
       61 tgagggggcc ccacccgtga agcggcgtgt cgcagtcacg ggtctcggcg tcgtgtcgcc
      121 gttgggcacg accgtcgcgg ccacgtggga agcgatggtg gcggggaagt cgggggcggg
      181 gccgatcacc cgcttcgatc cggccgcgct ctcggtcaag ttcgcctgcg aggtgaaggg
      241 cttcgatccc gggctctacc tcgacaagaa ggaagcgcgc cgctacgacc tgttcaccca
      301 gttcgccatg ggggcggcgg tccaggccgt caacgacgcc tgcctcgaca agagctggga
      361 cgggatcgac ctgcatcgcg tcggcgtcat catcggcacc ggcacgggcg gcatcgccac
      421 gttcgaagag aacatgcggg cctacctcga gaaggggccg ggccgcgtgt cgccgttctt
      481 cgtccccatg ttcatggcca acgtcgccgc ggcggtggtc tcgatgcgct atggcgccaa
      541 gggacccaac tactgcaccg tgtcggcgtg cgccacctcg gggcattcgc tgggggacgc
      601 cttccgcctg atccagcaca acgaggcgga cgtgatgatc gcgggcggct cggaggcggc
      661 gatcacgccg ctggcgctcg ccagcttcgc caacatgaaa gccctttccg agcgcaacga
      721 cgatccccgg accgccagcc gcccgttcga caaggaccgc gatggtttcg tcatgggcga
      781 cggcgccgcc gtcgtcatcc tcgaggaatg ggagcacgcc aggcggcgcg gcgccaagat
      841 ctacgccgag atggtgggct acggcatgac cgccgacgcc taccacatca ccgccccgtc
      901 ccccgacggc gagggtgcgc agcgtgccat gcgcctcgcc atggaggacg gcggcatccg
      961 gcccgagcag gtgggctaca tcaacgccca cggcacctcc accccgcact gcgatgccgc
     1021 cgagaccgcc gccgtgaagg gcgtgttcgg tgctcaggcg accaggctcg tcttcggctc
     1081 caccaagtcc atgaccggcc acctgctggg tgcggcgggc gcgctcgagt tcgccgtgtg
     1141 caccctcgcg ctgcacgagg gtgtgatccc gcccacgatc aaccagttca cccccgatcc
     1201 ggcctgcgac ctcgactcgg cgcccaatca ggcggtgacg cggcaggtcg aggtggcgct
     1261 ctccaattcc ttcggcttcg gcgggcacaa cgtcacgctc gccgtgcgcc gcgcgccgtg
     1321 accgccgggg cgcgcgatcc ggcgctagcg ccgatcgtcg agaaggtcga gcgcggcgag
     1381 cgcctcaccc gtgcggacgc gctcgccctc ttcgcttcgc atgatctcct gaccatcggg
     1441 gagctcgccg accgcgccaa ccggcgtcgc aacggcgacc gcgtcttctt cgccgccaac
     1501 cagcacctca acccgaccaa cgtctgcatc ctgcgcaaca cctgcgtgtt ctgctccttt
     1561 gcgcgcatgc cgaacgagga agggagctac acccgcaccc tggacgaggt cttcgccgag
     1621 gccgacgcgg cgcgcgacaa ccccacccgc gagtttcata tcgtcggcgg gctgcacccg
     1681 aagctgtcgc tcgagtacta cctcgacatg ttccgggggc tgaagactcg tcaccctgac
     1741 gtccagatca aggcgctcac cgcggtcgag gttgcgcacc tggcgcgtat cgagggcatg
     1801 accacgcgcg acgtgctcct cgccatgaag gacgccggcg tctcgagcct cccgggcggc
     1861 ggcgccgagg tgttcagccc cgcggcgcgc gctacaattg ctgatagaaa gctcaccggc
     1921 gaggagtggc tcgccgtgca ccgcgaggcg caccggctcg gcatcccgtc caactgcact
     1981 atgctctacg ggcacgtcga gacgctggaa gaccgggtgg accacctgct ggcgctgcgc
     2041 gcgctgcagg acgagaccaa cgggttcctg acctacatcc cgctcgccta tcaccccgat
     2101 cacaacgagc tgggggaggc gctgggccgc accggcaccg ccaccaccgg ctgcgacgac
     2161 ctgaagaaca tcgcggtggg ccgcctcgtg ctcgacaaca tcccgcacgt gaagacccac
     2221 tggcccatgg tgacgcccta catctcccag gtctcgttga gcttcggctg cgacgacctc
     2281 gagggcaccg tagtgttcga gcgcgtctat cacgaggcgg gcgccggtac tccgatgtgg
     2341 ctctcctacg acgagatcat cggactgatt cgcggggcgg ggaagacgcc ggtcgagcgc
     2401 gactcgctct atcagaccgt ccggaccttc gaagattggg ttcccggcac cgcggcaaag
     2461 ccgcgcgccg atggtcccga gggaccgacc tttcacatcg agcaccgggt cacggctcgc
     2521 ggcaagggga aacggcagct gccagtggta gctggagtgg tggaggggtt ggaatgatga
     2581 gccatgccgt agctctcccc cactcgaatg gtgattcccc ctctcccgat agggagaggg
     2641 ggccaggggc agaggtagca tgagactggg ccggattccc tacatcaact gctacccggt
     2701 ctacggcgcc atcgaccggg ggctggtcgg cgttccggcc gacctggtga ccgggactcc
     2761 ggccgagctg aacgacctgc tcgtggcggg cgagctcgac gtctcggtca tcagcgccgt
     2821 cgcctacgcg cgccatgcca aggacctggt gctgctcccc gagctggcga tctcgtgcga
     2881 cggcccggtc cagagcgtca tcctgttcag ccgccggcct gccgagaagc tcgacggccg
     2941 ggccgtgctg cggagcgcct cctcccgcac ttccgtggcg ctgctcgagc tgctgtgtcg
     3001 cgacgtctgg aagatccgtc cccgctttgc cgaggcgcgt gccgaggcgg ccgacctgga
     3061 gcagctggcc ggcctgccgc acgacgccgt gctcgtcatc ggggaccccg cgctgctgct
     3121 cgccgcgcgg cacgtgtacc cgtaccgcta cgacctgggc gaggagtgga agcgctggac
     3181 cgggctgccc ttcgtcttcg ccgtctgggc cgcgcgccgc ggcgccgatg cgcaggcggt
     3241 gcggcgcatt catcgcgcgc tgctcgaatc ccgcagctgg gggttggcgc accaggacga
     3301 gctggcccgg gccgcttctg ccgcgaccca ggtgcccgag cccgagtgcc gtgcgtatct
     3361 gggcgggctc gactacgcgc tcacctacaa acacctcgcc gggctcaccg acttcttccg
     3421 ccgtctggcg gcggccggac tcgtgcccga cggctcgctc cagttcctgc aggtggcctg
     3481 acatgcccga cgttatcgat cggacctccg ccgagttccg ctcccacctc gagctgtacc
     3541 agcgcgcgcc gctgctcgaa ttgggccggt tggccgacga cgcacgctgg cgcctgcacc
     3601 ccgagccggt cgtcacctat atcatcgacc gcaacatcaa ctacaccaac gtctgcgtcg
     3661 ccgactgcaa gttctgcgcg ttctaccggc gaccgaagca tctcgagggc tatgtcctgt
     3721 ccttcgagga gatcggccgc aagatcgagg aagtgaaggc cctgggcggg gtacagatcc
     3781 tcatgcaggg cggccacaat ccctacatcc ccttcgagtg gtacctcgac ctgctgcgtt
     3841 acatcaagcg caatcacccg atccacatcc acgccttctc gccctccgag gtcgatttct
     3901 tcgccgggcg cttcgggatg agcatcgagg aggtcctgaa ggagctggtg ggggcggggc
     3961 tggactcgat tcccggaggg ggtggggaaa tcctggtgca ggacgtacgt gaccgcgtcg
     4021 ccaaaaagaa ggccggggcc gaccgctggc tcgaggtcat ggagatcgcc caccgccagg
     4081 ggttgaagac cagcgtcacc atgatgtacg gcctgggcga atccgacgcc gaccgcatcg
     4141 agcacctcca tcgcgtccgg gcggtccagg cgcgcaccgg cggcttcacc gcgttcatct
     4201 gctggccgct ccagcccgag aacaccgagc tcgagtacct gggaaagacc gatgcggtca
     4261 cctacctgcg cacccaggcc atcgcccgcg tcgtgctcgg caacgtcccc aacatccagg
     4321 cctcctgggt caccatgggc cccaagatcg gccaggtcgc cctgcgcttc ggcgccaacg
     4381 acttcggctc cctcatgatg gaggagaacg tcgtctccgc cgccggcacg acgtggcgca
     4441 tgaccctgcc cgaaatgcag cgcctgatcg gcgatgcggg atacaccccc gtcaagcgcc
     4501 ggcaggatta cacgctactg tccgaagccg cctagccgat gaccgatagc cgataggcga
     4561 tagccctatg cccgacgtcc tcatcctcgt cggctccgag agcgacaagc cgcgcatcga
     4621 gccggccttc gaggtcctcg acaaggccgg cgtgagctac gagtttcacg tctcctcggc
     4681 ccaccgtact cccgaccgca ccggcgagct ggtcaagggc gccaaggcgc gaggcgtgcg
     4741 cgtgctcatc tgcggggcgg ggctctccgc cgcgctcccg ggcttcgccg cggcgctcac
     4801 cgatctcccc gtcatcggcg tgcccttcgc cgtggggccg ttgaacgggc tcgacgcgct
     4861 gctcgccacc gtccaggtgc cgcccggcgt cccggtcgcc accgtcggta tcgacaacgc
     4921 caagaacgcg gcccatctgg cgctgcgcgt gctgcagcgc tgaccgtgcg catcggcatc
     4981 ggctacgact cgcaccgctt cgccgagggc cgggcgctcg tcctgggcgg cgtttccgtt
     5041 ccccatcacg tcggcctgga cggctggtcc gacgccgacg ccgtggccca cgcgctgatc
     5101 gacgccatcc tgggcgcagc cgggcagggg gacatcgggc agatgttccc ctctgacgat
     5161 cccaagtgga aggacgccgg ttccctcgac ctgctgcggc gcgccagcct caaggtcgcc
     5221 gagctcgggc tgtctctggc ccacgccgac atcaccatca tcgccgagca gcccaggctc
     5281 gcgccctacg tggaccagat gcgcgagaag ctcgccgatg cggccgtggt cggcttctcc
     5341 aatctctccg tcaaggcgaa aaccaacgag gggatgggat tcatcgggcg gggcgagggg
     5401 gtagccgtca tcgccgtggc cctgctcgag gaggcgcgga cctgaccgat ctgctgcggt
     5461 ccctcgagtc tctccctccg gtcccgctgt acgccatcat cggtgccttc gccgccctcg
     5521 aaaacgtctt cccgccggcc cccgccgatg tcgcgatcgc gctgggcgcc tttctcgccg
     5581 ggcgcggcac cctcgatccc tggatcgtct tcgccgtcac ctgggtcggc aacgtcggca
     5641 gcgcgtccct cgtctatgcc gtcgggcgcc gttacggccg ggcgttcttc acggggcgga
     5701 tcggccgccg gctcttgtcc gagagcaccc tggcgcacat cgaggaggcg tacgcgcggc
     5761 acggcacctg ggggatattc gtctcccgcc tgctccccgt atggcgcgcc gtggtgcccc
     5821 cctttgcggg cgtcgcccgg gtacctgcgg cccgggccat tctctcgatg gccgtggcgt
     5881 cggccctgta ctacgggacc ctgaccttcc tcgtcgccac cttgggcacc aacttcgacc
     5941 tggtcacccg cgtcctcgcc cgggccaatc tcgtgctcgg cgtggtcgcc gtcgtcctca
     6001 tcctcctgtt cgtgcgatgg agctaccggc ggctgaaaca gtagacattc ccagggcctt
     6061 tcgcctcgag cagttccgcg actacttggc gctcgaggtg gggtcgagcc gccacacggt
     6121 cgagagctat ctgcgcgacg tgcgccgcct cgcgacccat gctgccggtt tggggatcga
     6181 ccagcccgac cggctcaccc ccgtacaact gcgcgagttc gtctacgccc tgaaggacct
     6241 cggcctctcc ccggccacca tccatcgcca gatttccgcc attcgcggct actttcgctt
     6301 tctcgtcggt gagggacatg cggcccgcaa ccccagcgag cgcctcgagt cccccaagcg
     6361 ttggcgcacc ctccccactg tgctcaccgc gcacgagatc gaccgcctgc tcaccgcgcc
     6421 cacccccgac acccctctcg gcatccggga ccgggcgttg ctcgagttcg cctatgccac
     6481 cggggcgcgc gtctccgagg tcgtcggggt cagcctccag gacgtcctct acgaggaggg
     6541 cctggctcgg ctcttcggca agggatccaa gcagcggctc gtccccgtgg ggcggcgcgc
     6601 gctcggcgcc gtggcgctct acgcccggga aatccggccc cgtctcgatc gcggcaagac
     6661 ccggggcgtc ctgtttctca acgcgcgcgg caccccgctg agtcgcgtcg gggcctgggg
     6721 cgtcattcgg caggcggcca aacgtgcggg catcgccaag cgcgtgtcac cccacaccct
     6781 gcgccacacc ttcgccaccc atctcctcga gggtggggct gatctgcgtg cggtgcagga
     6841 gatgctcggc catgtcgatc tggctaccac ccagctgtac acccatgtgg accgcgacta
     6901 cctgcggtcc gtccaccgcc agtttcaccc ccgtgcgtga gccggcttcc gcgggggggg
     6961 acggaacacg tttcttgccg tcggcgttga ccgacacaac agcttggagg atcacgtgct
     7021 gagcgactat ctcgtaggtg tgatcgtgcg ggacatgaag gcgttggggc gtgaggtcga
     7081 ggcctatccc gacgacgcca gcctgtggcg tacgccgccg ggcatcgcca attcaggcgg
     7141 caccctggcg ctccatctcg ccggcaacgt ccagcacttc tttggtgccg tcctgggcgg
     7201 caccggatac gtgcgcgatc gcaacgccga gttcagccgg cgcgatgtgc cgcgcagcga
     7261 gatcgcggcc cagatccagg cggccgtcgc cgccgccgag cgcggcctgg cccaggccag
     7321 cgatgccgtc atggcgcgtg actatcccga gcagatcgcg ggacacacca tcgtcaccgg
     7381 tgagtggctc gtccatctcg tcgcccatct cgggtaccat ctcgggcagg tcgattacca
     7441 tcggcgactt gtcaccggga atggcgagac cgtgaaggtc atggcggcgc cggaactgag
     7501 gacggctcgg aaacaataag agggacgggg gacgagggac gggggaaggg ttaaggcgag
     7561 gagagcgcca cgctctcgat ccggaccagg ccgaggggtg tgtagtactt ccagtccggt
     7621 ttcagcccga tgtcgcggat ttccacgcgc aggcggcgca gcgtcccgtc cgcgtcgcgg
     7681 cagaaccagg tgaacttcac gtcttcgacc cactgacctc tgtcccaggg caggcgggat
     7741 ag
//
LOCUS       DBNGZW010000496         3386 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_798425, whole genome shotgun sequence.
ACCESSION   DBNGZW010000496 DBNGZW010000000
VERSION     DBNGZW010000496.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_798425"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..730
                     /locus_tag="AC16JC_11655"
     CDS             <1..730
                     /locus_tag="AC16JC_11655"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5165394.1"
                     /translation="VDSLAARRVDFIKVHEGLSREAWFAIARAAHQHGIPLTGHVPAG
                     LSPDEASDSGLTSIEHLEFLPDRCLVLFDSTARARHTARPVGCRPPDLQRLLQHLRRN
                     GVWLDPTIVSFRVFAPRQFPSILAGFTELAGMIRQAGVPVLAGTDLGSAGMAPGETLH
                     DELGLLVAAGYSTADALRAATLNPAIFLGAADSMGRADSGFVADLVVLAGNPLADIRN
                     TMRIVAVIRAGRLLSSAALDSLRH"
     gene            805..1242
                     /locus_tag="AC16JC_11660"
     CDS             805..1242
                     /locus_tag="AC16JC_11660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LIC_13387 family protein"
                     /protein_id="HIR5165395.1"
                     /translation="MNSAAFYRISAFLLVLFAGGHTIGFRRVDPRWGVEAVVSSMQTI
                     RFDVQGMSRTYWGFYVGFGLFVTVLLLFAAVLAWQLSRLPAEVLYAVPLLTWGFALSF
                     AVVTYLSWRYFFPAPVIFSAVIALCLVVAAWLSGRPTSSTRIG"
     gene            1275..1946
                     /locus_tag="AC16JC_11665"
     CDS             1275..1946
                     /locus_tag="AC16JC_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020638594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR5165396.1"
                     /translation="MHRTIEPTILYFGTPVALISTLNEDGTPNIAPMSSAWWLGWSCM
                     LGLGTMGQTSENLLRTRECVINLPSDEQVAHVDRLALTTGRNPVPAKKVTWGYRYEPD
                     KFGTAELTQQPSLVVRPPRIAECPVQLEGVVHDVRPFGKNVSANAFEVHIVKLHVEES
                     LLDLSGPRPYIDAERWRPLIMSFTRFFGLGGEVHPSRLAESDFMTFVRGGTGPRPEVA
                     SSRSS"
     gene            1992..>3386
                     /locus_tag="AC16JC_11670"
     CDS             1992..>3386
                     /locus_tag="AC16JC_11670"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020486458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR5165397.1"
                     /translation="MNRPPVRFAAAVAACAAALPLAAQRAPAPEPLAAFTRHGISHDL
                     AIWRAHSIRDVRYDLSLDVTALDSAIGRVLVRFTRSGDDDVVLDYRGRRLQSVTVNGT
                     SLDPSAMGGGIPGFNGDHLRIPARLLTPGENAMDLAFVSDIAPTGASIIRVRDPADRS
                     DYLYTLLVPADADQLFPCFDQPDLKARVALHLTVPRAWTALANGSIARADTGGDRVTD
                     HFTETKPLPTYLIAFAAGPWASVASTAHGRTVHVFMRRSRAQEADADTLLALQQRALD
                     WMESYFGRPFPFEKFDMLLAPAFPFGGMEHPGVVMYNEDRFIFRERPTLPRRLGRFST
                     ILHETAHQWFGDLVTMRWFDDLWLKEGFATYMAAKALDELEPSSEAWKTFYQSNKPAA
                     YGVDQTTGTTPLWQAMDNLDQAKSAYGAIVYNKAPSVLKQLDYLVGEAAFQAGVRSFL
                     DAHAYGNATWRDLLG"
BASE COUNT          501 a         1203 c         1106 g          576 t
ORIGIN      
        1 agtcgactcg ctcgcggccc ggagagtgga tttcatcaag gtgcacgagg gactgtcgcg
       61 cgaggcgtgg ttcgccatcg cacgcgccgc gcatcagcac gggataccgc tgacgggaca
      121 cgtgccggcg gggctcagcc ccgacgaggc ctcggactcg gggctcacga gtatcgagca
      181 tctggagttt cttcctgacc ggtgtctcgt gctcttcgat tcgacggcgc gcgcgcgtca
      241 caccgcgcgg ccggtgggct gccggcctcc cgaccttcaa cgtctgctcc agcacctccg
      301 gcgcaacggc gtctggctcg atcccaccat cgtcagcttc cgcgtcttcg ccccgcgcca
      361 gttcccgagc atcctggccg gattcaccga gctggccggg atgatccgtc aggccggcgt
      421 gccggtgctc gcagggacgg atctcggcag cgcggggatg gccccgggag agacgctcca
      481 tgatgagctc ggcctgctgg tcgccgcagg gtactcaacg gcagacgcat tgcgcgctgc
      541 gacgctcaac ccggccatct tcctcggtgc ggcggacagc atgggacgcg cggactcggg
      601 gttcgtcgcc gatctcgtgg tgctcgcggg gaacccgctc gccgacatcc ggaatacgat
      661 gcgcatcgtc gcggtgatac gcgcggggag gcttctctca tccgcagcgc tcgacagcct
      721 gcgacactaa ccggccggcg ggcggatcgg gtacctcgcg ttcgtcgaat gagggaggag
      781 tccaacccat catgaggacc agttatgaac agtgccgcat tctaccgcat cagcgctttc
      841 ctcctcgtcc tcttcgccgg cggccacact atcggctttc gtcgcgtcga tccccggtgg
      901 ggcgtcgagg ccgtcgtctc gagcatgcag acgatccgat tcgacgtgca ggggatgtcg
      961 cgcacctatt ggggcttcta cgtcgggttc gggctcttcg tcaccgtcct gctgctgttc
     1021 gccgccgtcc tggcctggca gctgagccgg ctccccgcgg aggtcctgta cgccgtgccg
     1081 ctcctcacct ggggcttcgc cctgtccttc gccgtggtca cgtatctcag ctggcgctac
     1141 ttcttcccgg cgccggtgat cttctcggcg gttatcgccc tctgcctggt cgtcgcggcc
     1201 tggttatcgg gccgtcccac ttcgagcaca agaatcggat agatcgtgac ggccggcggc
     1261 attaacctgg ccgcatgcac agaacgatcg agccgacgat cctctatttc ggcacgccgg
     1321 tggcgctgat ctcgaccctg aacgaggacg gcacccccaa catcgcgccc atgtcctccg
     1381 cctggtggct cgggtggagc tgcatgctgg gactcgggac gatgggccag acctccgaga
     1441 acctgctccg cacgcgcgag tgcgtgatca acctgcccag tgacgagcag gtcgcccacg
     1501 tggaccggct ggcgctcacg acgggacgga acccggtgcc cgcgaagaag gtcacctggg
     1561 gctaccgcta cgaacccgac aagttcggca ccgcggagct gacccagcag ccgtcgctcg
     1621 tcgtccgccc gccgcgcatc gcggaatgtc cggtgcagct ggagggggtg gtgcacgacg
     1681 tgcggccgtt cggcaagaac gtgtcggcca acgcgttcga ggtccacatc gtgaaactgc
     1741 acgtcgagga atcgctgctc gacctcagcg gaccgcgtcc ctacatcgac gccgagcggt
     1801 ggcgtccgct catcatgagc ttcacccggt tcttcgggct gggcggcgag gtccatccgt
     1861 cccgcctggc cgaatcggat ttcatgacgt tcgtgcgcgg cggcaccggg ccgcggccgg
     1921 aggtggcgag ctcgcgctcc tcctgaggaa catccgggtg cacggtcggc acgccgcgct
     1981 aagttgtcgt catgaaccga cccccggtgc gattcgcggc ggcggtggcc gcgtgtgccg
     2041 ccgccctgcc cctcgccgcg caacgcgcgc cggcccccga accgctcgcc gcgttcacgc
     2101 ggcacggcat ctcgcacgac ctcgccatct ggcgcgcaca cagtattcgg gacgtccgct
     2161 acgatctctc gctcgacgtc accgccctcg acagcgcgat cgggcgggtg ctggtgcggt
     2221 tcacgcgcag cggcgacgac gacgtcgtcc tcgactatcg cggccggcgc ctgcaatcgg
     2281 tcaccgtcaa cggcacgtca ctcgacccgt cggccatggg cggcggcatc cccggcttca
     2341 acggcgatca tctccggatc ccggcccgac tcctcacccc gggcgagaac gccatggacc
     2401 tcgccttcgt ctccgacatc gcgccgaccg gcgccagtat catccgcgtg cgcgaccccg
     2461 ccgaccggag cgactacctg tacacgctgc tcgtgccggc cgacgccgat cagctgttcc
     2521 cctgcttcga ccagcccgac ctcaaggcgc gcgtcgcgct gcacctcacc gtgccgcgcg
     2581 cctggacggc cctcgccaac ggctcgatcg ctcgggctga taccggaggc gaccgcgtca
     2641 ccgatcattt cacggagacg aagccgctgc cgacctatct catcgcgttc gcggcggggc
     2701 cgtgggcgag cgtcgcgtcc acagcgcacg ggcgcacggt gcacgttttc atgcgtcgct
     2761 cacgcgcgca ggaagccgat gccgacacgc tgctcgcgtt gcagcagcgc gcgctcgact
     2821 ggatggagag ttatttcggg cgccccttcc cgttcgagaa gttcgacatg ctgctcgcgc
     2881 cggcgtttcc gttcggcggc atggagcacc ccggggtcgt gatgtacaac gaggaccggt
     2941 tcatcttccg cgagcggccc acgctgccgc gccggctcgg ccgcttctcc accatcctgc
     3001 acgagaccgc gcatcagtgg ttcggcgacc tcgtgacgat gcgctggttc gacgatctgt
     3061 ggctcaagga gggctttgcg acctacatgg cggcgaaggc gctcgacgag ctcgagccgt
     3121 cgagcgaggc gtggaagacg ttctaccagt cgaacaagcc cgcggcctac ggggtggacc
     3181 agacgaccgg gacgacgccg ctgtggcagg ccatggacaa cctcgaccag gcgaagagcg
     3241 cgtacggggc gatcgtctac aacaaggcac cgagcgtact gaagcagctc gattacctgg
     3301 tgggcgaggc ggcttttcaa gcgggcgtgc ggtcctttct cgacgcccac gcctacggca
     3361 acgcgacctg gcgtgacctg ctggga
//
LOCUS       DBNGZW010000497         1326 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1547010, whole genome shotgun sequence.
ACCESSION   DBNGZW010000497 DBNGZW010000000
VERSION     DBNGZW010000497.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1326)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1326)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1326
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1547010"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="AC16JC_11675"
     CDS             <1..858
                     /locus_tag="AC16JC_11675"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR5165398.1"
                     /translation="KFGAVGVRSGAGDLPQEEALPTATITFSLGEEAETVRWLHREVQ
                     ERETLPLAEAEAVVRSLSVAMHGDKQMMLPLLQLKQFDQYTTTHSLNVAVLSMALAEF
                     LGMGAADVRAFGVAGLLHDLGKVRIPIEVLTKPGKLTPDERVLMNTHPAEGARIILES
                     EQELDLAAVVAYEHHIMLNGLGYPQVKFRRPCHEASNLIHVCDVYDALRTRRPYREAW
                     SVEQVLPYIEGRAGLEFDPAYVRSFIAMMRKWESQVAVVSDERAPLPAAAAVTGAAGP
                     EASPESRPV"
     gene            875..1258
                     /locus_tag="AC16JC_11680"
     CDS             875..1258
                     /locus_tag="AC16JC_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165399.1"
                     /translation="MDKTSLLLAICALGATGLSAQRATGPRLEGKWEARTDDGPRLIM
                     VRADSSAQFGDQVARWRMVGDSVWFTLGDGVWNVYGLRVAGDKLTISGGDLEKPVTLR
                     RVGPATARPDTLKVPEPPPANARAW"
BASE COUNT          210 a          422 c          486 g          208 t
ORIGIN      
        1 aagttcggcg ccgtgggcgt tcgcagcggg gcgggggacc tgccgcagga ggaggcgttg
       61 ccgaccgcca ccatcacctt ctccctgggc gaggaagcgg agaccgtccg ctggctgcac
      121 cgcgaggtgc aggagcgcga gacccttccc ctggccgagg cagaggccgt ggtgcgctcc
      181 ttgtcggtgg cgatgcacgg cgataagcag atgatgctcc cgctcctcca gctcaaacag
      241 ttcgatcagt acacgacgac gcactccctg aacgtcgcgg tgcttagcat ggcgctggcc
      301 gagttcctgg ggatgggggc cgccgacgtg cgcgcgttcg gcgtggcggg gctgctgcac
      361 gacctgggca aagtgcggat tccgatcgag gtgctcacca aacccggcaa gctcacgccg
      421 gacgagcgcg ttctgatgaa cacgcacccg gccgagggcg cccgcatcat tctcgagagc
      481 gagcaggagc tcgacctggc cgcggtggtg gcgtacgagc accacatcat gttgaacggc
      541 ctgggctatc ctcaggtgaa gttccggcgg ccgtgccacg aggcgagcaa cctgatccac
      601 gtctgcgacg tctacgatgc gctgcggacc aggcggccct atcgggaggc ctggtcggta
      661 gagcaggtgt tgccctacat cgaggggcgg gctgggctgg aattcgatcc ggcttacgtg
      721 cgatccttca tcgcgatgat gcggaaatgg gagtcgcagg tcgccgtcgt ctccgatgag
      781 cgcgccccgc tgcccgcggc cgctgcggtg acgggcgccg ccggtcccga agcgagcccc
      841 gagagccgtc ctgtttgatt aggtttcgga cacgatggac aagaccagtc tgttgcttgc
      901 gatctgcgcg ttgggcgcta ccggactgtc cgcgcagcgg gcgaccggac cacgcctcga
      961 gggaaaatgg gaagcgcgta ccgacgacgg cccccggctc atcatggtgc gcgccgacag
     1021 ctccgcccag ttcggcgatc aggtggcgcg ctggcggatg gtgggggaca gcgtctggtt
     1081 cacgctgggg gacggcgtct ggaacgtcta cggcctgagg gtcgcgggcg acaagctcac
     1141 catctccggg ggcgatctcg agaagccggt gacgctgcgt cgcgtgggac cggccaccgc
     1201 ccggccggat accctcaagg tccccgagcc gccgccggcc aacgcgcggg cgtggtaggg
     1261 gcggggagtg gggagtgggg agtagacaga gccgatcttt cgtgcgaccc gtcaggtacc
     1321 cctccc
//
LOCUS       DBNGZW010000498         5379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1341291, whole genome shotgun sequence.
ACCESSION   DBNGZW010000498 DBNGZW010000000
VERSION     DBNGZW010000498.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1341291"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..348)
                     /locus_tag="AC16JC_11685"
     CDS             complement(<1..348)
                     /locus_tag="AC16JC_11685"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5165400.1"
                     /translation="MRWWRPAIHLAATALAAACTSPGKGMIALEGGTLIDGSGGPPKA
                     DALILIKDGTIRAVAQVNEIKVPRGAQRIDLVGKTIMPGLIDAHAVVERWALPRYLAW
                     GVTSVRAVHSVGDS"
     gene            complement(345..794)
                     /locus_tag="AC16JC_11690"
     CDS             complement(345..794)
                     /locus_tag="AC16JC_11690"
                     /inference="COORDINATES: protein motif:HMM:NF024865.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR5165401.1"
                     /translation="MRGTWWVAALAAACAAPKIPTGDPAREIPAMLARSAADWNRGDL
                     DGFMGDYVKDSTVGYVSGGHVQYGWQPLYDHYKATYFAPGKSRDSLSFVEIAVRPLTM
                     DLAYTTARFELHRGDSLTASGPFTLILQKRGDRWQILHDHTSADPKP"
     gene            838..1584
                     /locus_tag="AC16JC_11695"
     CDS             838..1584
                     /locus_tag="AC16JC_11695"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR5165402.1"
                     /translation="MADATPPRGSIVYGPVHSRRLGASLGVNLLPARSKVCSFDCPYC
                     ECGFNTGKAVDARWPSPDEVGHALRRALARLPAAPAWITLSGSGEPTLHPRFAVAVDR
                     VLTARDELAPRTRVGALSNGVLGAVPAIRGALLRLDGRFLKLDPGPPELVNGVAGDAG
                     ARADSYRALRPVTVQAMVVRGPGWDGASAASLAAWLPLLVRAEPAAVQLYSLARPPAD
                     PRVRNVPRERLLAMAAAIREALPRTVVEVF"
     gene            complement(1591..1881)
                     /locus_tag="AC16JC_11700"
     CDS             complement(1591..1881)
                     /locus_tag="AC16JC_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165403.1"
                     /translation="MLATSDLTGMGGPGMMEPMFPPEPSMTPEPAPMMDEPMTEEAPR
                     PKPRKAPAKKKKAKRPAAKKAKKAKKPARKAKRPKARKPARAKRKVARRRRR"
     gene            1956..4580
                     /locus_tag="AC16JC_11705"
     CDS             1956..4580
                     /locus_tag="AC16JC_11705"
                     /inference="COORDINATES: protein motif:HMM:NF036578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HIR5165404.1"
                     /translation="MRQLLGGFLLLPTFLTAQVRPDSTAILQRAPISLPWRRFDVTLT
                     GPVRGSGYVSAIGRRAALIGTDAGSFEAWTWPLKLLHDFSLSFKTPLYDQPIPGKDIA
                     RRVEISPAGATIIYTHSAFTVTERLFAPLDQPAVVAVLEVDAVRPIEIIARFRPDLQL
                     AWPAGFGGQYVFWDDAERAFVLSESRRQVNGFFGSPAATWATNNPSHMLADASSELHI
                     AVGDQGPVAMPRPGEPAGRLTEVHAAGIPIVMVGAVAPRDSVRAIYRRVLGHLAELYA
                     ARADHAQRVLDSTVVFHSPDATLDLAVRWASLNLDEAMVCNPDLGCGLVAGYGPSGAT
                     GTRPGFGWFFGGDAAINSFAMSSAGLWPVARDGLAFFAGYQGKTPPNAGKIPHEISQG
                     AARIRWFEDYPYAFYHGDTTPFWMLAVAEYWRASGDSATVRRLWPNLKKAYDWAKGTD
                     ADGDGLMDNAKAGAGAIEVGDLQKGLQSDIYMSAVWVRALEVFPQMADLAGDRAAAAE
                     ARRLAARARGSLREKLWLPAEGRYAFALLDDGTRSTELTVWPATGLAFGLFDDDRGMA
                     TAATLARAAITTDWGARALASTSPLFDPLHYNNGTVWPFVTGFDAWGLYRAHNAPMAF
                     AATSAVARTTFLWGLGANPEVFSGSTFEPLETAVPQQFFATSMLLTPLVRGLVGWEGD
                     VPDDRVTLSPHLPPQWDSLAVERLPVGTGRYTVQFARTDTTLAMELTRTAGTGVDTLV
                     FSPALPLGAVVREVRANGRSVTCAARSSGADEHVDCRLALGSRLVVLIRHAPGWAVLL
                     PPPAPERGARSTSMKIVSQRLAGDTLELQLEGLAGKAYELVVRTPRGERRMPVTFPDS
                     GDAVDGYRAKAIRVTAQP"
     gene            4601..>5379
                     /locus_tag="AC16JC_11710"
     CDS             4601..>5379
                     /locus_tag="AC16JC_11710"
                     /inference="COORDINATES: protein motif:HMM:NF014427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5165405.1"
                     /translation="MISRVCLPAVLLVAAAGASLAAQTVAEIQVAPPSISIKVGERYG
                     LLATAYDRVGNVLPAARVTWVSNNPQVAKVDNTGTVLGVAAGATIVEARSGQRRGQAV
                     VQVGAVTGTGEAGQAGGAAVDPLAGQPAGTGPAAVLRIDPPSVYMLPSEHTRVSPRAL
                     REDGTPAAPVQVTWKSLREDIASVDQNGTVVALAPGQGTIQMSGSGGLTATAPVVVQQ
                     AEYAVQEGKALTFAPGEVDTLHVVVPTQENRVVNPVALTWAS"
BASE COUNT          712 a         1947 c         1876 g          844 t
ORIGIN      
        1 ggaatcgccc accgagtgga cggcccgcac cgacgtcacg ccccaggcga ggtagcgggg
       61 cagggcccag cgctccacca cggcgtgcgc gtcgatcagt ccgggcatga tcgtcttgcc
      121 caccagatcg atgcgctgcg ccccgcgcgg caccttgatc tcgttcacct gggccactgc
      181 ccggatggtg ccgtccttga tgaggatcag ggcgtcggcc ttgggaggac cgcccgagcc
      241 gtcgatcagc gtccccccct cgagggcgat catgcccttg ccgggcgagg tgcaggcggc
      301 ggcgagcgcc gtggcggcga ggtggatcgc cggccgccac cacctcacgg tttcgggtcc
      361 gccgaggtgt ggtcgtgcag gatctgccag cggtcgccgc gcttctggag gatgagcgtg
      421 aacggcccgc tcgctgtcag cgagtcgccc cggtgcagct cgaatcgcgc ggtcgtgtag
      481 gccaggtcca tggtgagggg ccgcaccgcg atctccacga aggagagcga gtcccggctc
      541 ttgccgggcg cgaagtaggt cgccttgtaa tgatcgtaca gcggctgcca accgtattgg
      601 acgtggccgc cgctgacgta gccgacggtc gaatccttca cgtagtcgcc catgaagccg
      661 tccagatcgc cgcgattcca gtccgcggcc gaccgcgcca gcatcgccgg gatctcccgg
      721 gccgggtccc cggtcgggat cttcggtgcc gcgcaggccg ccgcgagggc cgccacccac
      781 catgtgccac gcatccgcac ctcttcgttc gctggccgcc agaacgtacc ttgccgcatg
      841 gccgatgcaa ccccgccgcg cgggtccatc gtctacggcc ccgtgcactc gcgtcgcctg
      901 ggcgcgagcc tgggcgtgaa cctgctgccg gcgcgttcca aggtgtgcag cttcgattgt
      961 ccctattgcg agtgcgggtt caacaccggg aaggcggtcg acgcccgctg gccctctccc
     1021 gacgaagtgg gccacgccct gcggcgcgcg ctcgctcgat tgccggcggc ccccgcatgg
     1081 atcaccctct ccggaagcgg cgagccgacc ttgcatccgc gattcgcggt tgccgtggac
     1141 cgtgtgctga cggcgcgcga cgagctggcc ccgcgcaccc gggtcggtgc gctctcgaac
     1201 ggcgtcctgg gggccgtccc ggcgattcgc ggggcgctgc tccggctcga cggacgtttc
     1261 ctgaagctcg atcccgggcc acccgagctg gtcaacggcg tcgccggcga cgctggcgcc
     1321 cgcgccgact cctaccgggc gctgcggccg gtcacggtgc aggcgatggt ggtgcgcggc
     1381 ccgggctggg acggtgcgag cgcggcgagc ctcgccgcct ggctgccgct cctggtgcgc
     1441 gccgagcccg ccgcggtcca gctctactcc ctcgcacgac cacccgccga tccgcgggtc
     1501 cggaacgtgc cgcgggagcg cctcctcgcg atggccgcag ccatccggga agcgctcccg
     1561 cggacagtcg tcgaagtctt ctaggtgccg tcagcgacgg cggcgacgcg cgaccttccg
     1621 cttcgcccgc gccggcttcc gggccttggg acgcttcgcc ttgcgggccg gcttcttcgc
     1681 cttcttcgcc tttttggccg ccgggcgctt ggctttcttc ttcttcgcag gagccttccg
     1741 cggcttgggc cgcggtgcct cctccgtcat cggctcgtcc atcatcgggg cgggctccgg
     1801 agtcatcgag ggctccggtg ggaacatcgg ctccatcatg cccgggccgc ccattcccgt
     1861 cagatcactc gtcgcgagca tctaagtcct ccgctggttg tggccgctgg gggcctatag
     1921 cttacccgcc taactccccg acgcaagtga gcgtaatgcg ccagcttctc ggcggttttc
     1981 tgctgcttcc cacgttcctt accgcacaag tccgtcctga ctccactgcc atcctgcagc
     2041 gggcgccgat ctcgttgccg tggcggcggt tcgacgtcac cctgacggga ccggtccgtg
     2101 ggtccggtta cgtcagcgcc atcgggcggc gggctgccct gatcggcacc gacgccggga
     2161 gcttcgaggc ctggacctgg cccctcaagc tactgcacga cttctcgctc tccttcaaga
     2221 cgccgttgta cgaccagccg atacccggca aagacatcgc ccgcagggtg gagatctcgc
     2281 cggccggggc caccatcatc tatacccact ccgccttcac ggtgaccgag cggctgttcg
     2341 cgccgctcga ccagcccgcc gtggtcgccg tcctggaggt ggacgcggtc cggccgatcg
     2401 agatcatcgc gcggttccgc cccgacctgc agctcgcctg gccggccggg ttcgggggcc
     2461 agtacgtctt ctgggacgat gccgaacggg cgttcgtgct gtcggaaagc cgccgccagg
     2521 tgaacggttt cttcggctcg ccggcggcca cctgggcgac caacaacccc tcgcacatgc
     2581 tggcggacgc atcgagcgag ctgcacatcg ccgtggggga ccagggcccg gtggcgatgc
     2641 cccggccggg ggagccggcg gggcgcctca cggaggtgca cgccgccggc atcccgatcg
     2701 tgatggtcgg cgccgtcgcc ccgcgcgaca gcgtgcgggc gatctatcgg cgggtgctgg
     2761 gtcatttggc cgagctgtac gccgcccgcg ccgaccacgc gcagcgggtc ctcgactcga
     2821 ccgtcgtctt tcattcaccc gatgcgacgc tcgatctcgc cgtgcgctgg gccagcctga
     2881 acctggacga agccatggtc tgcaaccccg acctggggtg cggtctggtg gcgggctacg
     2941 ggcccagcgg cgcaaccggc acgcgcccag ggttcgggtg gttcttcggc ggcgatgccg
     3001 ccatcaattc gttcgccatg agctcggccg gactgtggcc ggtggcgcgc gacggcctcg
     3061 ccttcttcgc gggatatcag ggcaagacgc cgcccaacgc cggcaagatt ccgcacgaaa
     3121 tctcccaggg cgcggcccgc atccgatggt tcgaggacta cccctacgcg ttctatcacg
     3181 gcgacaccac ccccttctgg atgctcgcgg tggccgagta ctggcgcgcg tccggggaca
     3241 gcgcgaccgt gcgccgtctc tggcccaacc tgaagaaggc ctacgactgg gcgaagggca
     3301 ccgacgccga cggcgacgga ctgatggaca acgccaaggc cggcgccggc gcgatcgagg
     3361 tcggagacct gcagaagggc ctgcaatccg acatctacat gtccgccgtc tgggtccggg
     3421 cgctggaggt gtttccccag atggcggatc tcgcgggtga ccgcgccgcc gccgcggagg
     3481 cgcgccgcct ggccgcgcgc gcgcgcgggt ccctgcggga gaagctctgg ctgcccgcgg
     3541 aaggacgcta tgcgttcgcc ctgctcgacg acggcacgcg ctccacggag ctgacggtct
     3601 ggcccgccac cggcctggcg ttcgggctgt tcgacgacga ccgcggcatg gccacggccg
     3661 cgacgctcgc ccgcgccgcc atcaccaccg actggggggc gcgggccctg gcatccacct
     3721 cgccgctgtt cgatccgctg cactacaaca acggcaccgt gtggcccttc gtcaccggat
     3781 tcgatgcgtg ggggttgtac cgggcgcaca acgcgccgat ggcattcgcg gcgacttcgg
     3841 ccgtggcgcg caccaccttc ctgtggggac tcggcgccaa tcccgaggtg ttctccggct
     3901 cgaccttcga gccgctcgag accgccgtgc cgcagcagtt cttcgccacc tccatgctgc
     3961 tcacgccgtt ggtgcgcggg ctggtcggct gggaaggcga cgtgcccgac gaccgggtga
     4021 cgctctcccc gcacctgccg ccccagtggg attctctcgc cgtcgagcgg ctgcccgtgg
     4081 gcacggggcg atacaccgta cagttcgcac gcaccgacac gaccctggcg atggagctga
     4141 cgcgcaccgc ggggacgggc gtggacacgc tcgtcttctc acccgcgctt ccgctggggg
     4201 ccgtggtgcg ggaggtgcgc gccaacggac gctccgtgac ctgcgccgcg cgctcgagcg
     4261 gcgcggacga acacgtggat tgccgcctgg cgctcgggtc ccggctcgtc gtcctcattc
     4321 gtcacgcgcc gggctgggcc gtgctgctgc cgccgccggc ccccgagcgg ggcgcgcgct
     4381 cgaccagtat gaagatcgtg tcccagcgcc tcgcgggcga tacgctcgag ctgcaactcg
     4441 aaggactcgc ggggaaagcg tacgagctcg tggtgcggac tccgcgcggc gagcggcgca
     4501 tgccggtcac gttccccgac agcggcgatg cggtggatgg gtatcgcgcg aaggcgatcc
     4561 gggttacggc gcagccctga gaagctagat tagcgtggcc atgatctccc gcgtgtgcct
     4621 gcccgccgtc ctcctggtcg ccgccgccgg cgcatcgctc gccgcccaaa ccgtcgccga
     4681 aatccaggtc gcgccgccgt cgatctcgat caaggtggga gagcgctatg ggctgctcgc
     4741 taccgcctac gaccgggtgg gaaacgtgct cccggcagcc cgcgtgacct gggtgtcgaa
     4801 caacccccag gtcgccaagg tggacaacac cgggaccgtg ctcggagtgg ccgccggagc
     4861 gacgatcgtg gaagcgcgct cgggacagcg ccggggtcag gcggtggtgc aagtcggcgc
     4921 cgtcacggga acgggcgagg caggacaggc cggtggcgca gccgtcgatc cgctggcagg
     4981 gcaacccgcc ggcaccggtc ccgcggcggt gctgcggatc gatccgccgt cggtgtacat
     5041 gctgccttcg gaacacacac gggtgtcacc acgggccctg cgggaagacg gcacgcccgc
     5101 ggcgccggta caggtcacct ggaagtcgtt gcgcgaggac atcgccagcg tggatcagaa
     5161 cggcaccgtc gtggcgctgg caccggggca gggcaccatc cagatgtccg ggtcgggcgg
     5221 actcactgcg accgcgcccg tggtcgtcca gcaggccgag tacgccgtgc aggaggggaa
     5281 ggcgctcacg ttcgcccccg gcgaggtgga caccctgcac gtcgtcgtcc cgacgcagga
     5341 gaaccgtgtg gtgaatccgg tggcgctcac ctgggcctc
//
LOCUS       DBNGZW010000499         4065 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_388066, whole genome shotgun sequence.
ACCESSION   DBNGZW010000499 DBNGZW010000000
VERSION     DBNGZW010000499.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4065)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4065)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4065
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_388066"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /gene="guaA"
                     /locus_tag="AC16JC_11715"
     CDS             complement(<1..635)
                     /gene="guaA"
                     /locus_tag="AC16JC_11715"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR5165406.1"
                     /translation="MNRILIIDYGSQFTQLIARRVREAHVYCEIHPASRGTDLAFIRE
                     FAPQGIILSGGPNSVFDPGAPTADAGVFDLGIPVLGICYGMHLITQLSGGTVTPAKER
                     EYGRADITVRENAGLFAGFDAGSTTTVWASHGDLIAAAPRGFRTTAASVNAPVAAFQH
                     ETKPISGLLFHPEVAHSVRGGEILANFLFGICRCTPDWTPGHFIDQSVDRIR"
     gene            complement(632..1882)
                     /gene="lysA"
                     /locus_tag="AC16JC_11720"
     CDS             complement(632..1882)
                     /gene="lysA"
                     /locus_tag="AC16JC_11720"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR5165407.1"
                     /translation="MGAGVLGFDPALLEAIAARVGTPAYVYGANLIRAQFHALDDALQ
                     GIPHTICYSVKANGNLAVLRVLQQLGAGADIVSVGELRRVHTAGFSSDRIVFSGVGKT
                     ADELEEAIRSRIGFLNLESTSELDAVIAAARRTGLRCRVGIRVNPDVAARTHPYTKTG
                     EKTAKFGVPFDDVVEVARRVAADPALELRGLAMHLGSQLTDVEPYHRGTVKLLELVAA
                     IRASGIDTLEVLDVGGGLGVRYFEERAPTPKAFAEAVVPPINAAGLRLLCEPGRYIVA
                     NAGLLLTRVLYRKHSGGKEYVIVDAGMTDFVRPSHYHAHHDIVPLRDGGPERVVNVVG
                     PICESGDFLALDRKLPAVESGALLAVLGTGAYGFVMSSTYNQRPRPPEVMVEGDKYFV
                     ARPRETLDDLMRGEVPEPAVWLSA"
     gene            complement(1864..3357)
                     /gene="proS"
                     /locus_tag="AC16JC_11725"
     CDS             complement(1864..3357)
                     /gene="proS"
                     /locus_tag="AC16JC_11725"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR5165408.1"
                     /translation="MAQAEKITPRAKDYSEWYNDVIMQAELADYSPVRGCMVIRPNGY
                     RIWELMQRALDDMFKAAGHQNAYFPLFIPMSFLAKEAEHVEGFAKEVALVTHTRLRAT
                     GKTGARAVELDPASALEEPLVVRPTSETIIYAMFAKWIQSYRDLPLLLNQWCNVVRWE
                     LRTRLFLRTTEFLWQEGHTAHADEADAEREARLILGIYRTFVEEWLAMPVLTGVKTDT
                     EKFAGALRTYAIEALMQDRKALQAGTSHNLGQNFARAFNVQYQTAAGGLEYVWSTSWG
                     VTTRLIGGLIMTHSDDNGLVCPPRLAPVQVVLVPIWKSDEERARVLEVATRVEGELKA
                     AGLRVVLDARDGLKPGAKYFEWEARGVPLRFEIGPKDVAAEHAMAARRTGGKAPVPLA
                     GIAATAAQALEEIQAGLLAAARERREANSIRGVTKEKFIEFMQDQGGFAYGGFCGGGE
                     CEAAIKDVTGATIRVLPDAEFRSPEAPRTCMWCGKPSVAEAVWAQAY"
     gene            complement(3396..3755)
                     /locus_tag="AC16JC_11730"
     CDS             complement(3396..3755)
                     /locus_tag="AC16JC_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011550211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5165409.1"
                     /translation="MDKVNLAEKLARVNEYWSPKIVGELNGQQVKLVKFIGDFVWHHH
                     DQEDELFYVVKGRFRMEFRDRSVDVAAGEFLIVPRGVEHRPVAEHEVEVMLFEPATTL
                     NTGNVRNERTVEVLERL"
     gene            complement(3748..>4065)
                     /locus_tag="AC16JC_11735"
     CDS             complement(3748..>4065)
                     /locus_tag="AC16JC_11735"
                     /inference="COORDINATES: protein motif:HMM:NF015113.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5165410.1"
                     /translation="AVPAGSSRLDLFLVAVGGEDLATVLRLAHELRGRGLAVEYGLRL
                     QAVRKQLELAASRGAPRAVIIGPNERAAGQVVLRDLQRGTEERVAMAELGMHLQGKDG
                     PHG"
BASE COUNT          631 a         1473 c         1309 g          652 t
ORIGIN      
        1 cgtatccggt cgaccgactg gtcgatgaag tgtcccgggg tccaatccgg cgtgcagcgg
       61 cagatgccga acagaaagtt ggccaggatt tctccgccgc gaaccgagtg cgccacctcg
      121 ggatggaaca gcaggccgga gatcggcttg gtctcgtgct ggaacgcggc gaccggggcg
      181 ttcacgctgg cggccgtcgt gcggaatccc cgcggggcgg cggcgatgag gtcgccgtgg
      241 ctcgcccaga ccgtcgtcgt cgagcccgcg tcgaaccccg cgaacagacc cgcgttttcc
      301 cgcaccgtga tatccgcccg gccgtactcg cgctccttcg cgggcgtcac ggtgccgccc
      361 gacagctgcg tgatcagatg catgccgtag cagatgccga gcaccgggat gccgaggtcg
      421 aagacacccg cgtcggcggt gggcgcgccg ggatcgaaga cggagttcgg gccgcccgaa
      481 aggatgatcc cctggggggc gaactcccgg atgaacgcca ggtccgtgcc gcgcgacgcg
      541 ggatggatct cgcagtagac gtgcgcttcg cgcacgcgcc gggcgatcag ctgggtgaac
      601 tgggacccgt agtcgatgat caggatgcgg ttcatgccga cagccacacc gcgggctccg
      661 ggacctcgcc gcgcatcagg tcgtcgagcg tttcccgcgg acgcgcgacg aagtacttgt
      721 cgccttccac catcacctcg ggcggccggg gccgctggtt gtaggtcgag ctcatgacga
      781 atccataggc gcccgttccg agcacggcca gcagcgctcc cgactccacg gcggggagct
      841 tgcggtcgag ggccaggaaa tcgcccgact cgcagatcgg ccccaccacg ttcaccaccc
      901 gttccgggcc gccgtcgcgc agcggcacga tgtcgtgatg cgcgtggtag tggctgggac
      961 gcacgaagtc cgtcatcccg gcgtccacga tgacgtactc cttgccgccg gagtgcttgc
     1021 ggtacagcac gcgggtgagc agcaggcccg cgttggcgac gatgtaacgc cccggctcgc
     1081 acaacagccg cagtcccgcg gcattgatcg gcggcacgac ggcttccgcg aacgccttgg
     1141 gggtgggcgc ccgctcctcg aagtagcgta cgcccagccc cccgcccacg tcgagcacct
     1201 cgagcgtgtc gatgcccgag gcgcggatcg cggccacgag ctcgagcagc ttcaccgtcc
     1261 cacggtgata gggctcgacg tcggtgagct gggatcccag gtgcatcgcg aggccgcgca
     1321 gctccagcgc aggatccgcg gcgacccggc gcgccacctc gaccacatcg tcgaacggga
     1381 cgccgaactt ggcggtcttc tctcccgtct tggtgtacgg gtgggtgcgg gccgcgacgt
     1441 cgggattgac ccggatgccc acgcggcagc gcaggccggt gcgccgcgcc gcggcgatca
     1501 ccgcatcgag ctcgctcgtc gattcgaggt tgaggaaccc gatgcgcgag cggatggcct
     1561 cctcgagctc gtccgcggtc ttccccaccc cggagaagac gatgcggtcg gacgagaatc
     1621 cggccgtgtg cacccgccgc agctccccca ccgacacgat gtcggcgccc gcgccgagct
     1681 gctgcagcac gcgcaggacg gcgagattgc cgttcgcctt gaccgaatag cagatggtgt
     1741 gcggaatacc ctgcagcgcg tcgtccagcg cgtggaactg ggcgcgaatc aggttcgccc
     1801 cgtacacgta ggcgggcgtc cccacgcgcg ccgcgatcgc ctcgagcagc gcgggatcga
     1861 agcctagtac gcctgcgccc acaccgcctc ggccacgctg ggcttgccgc accacatgca
     1921 cgtccggggc gcctcaggcg aacgaaactc cgcatcaggc aggacccgga tcgtcgcgcc
     1981 ggtcacgtcc ttgatcgccg cctcacactc cccgccgccg cagaagccgc cgtaggcaaa
     2041 tcccccctgg tcctgcatga actcgatgaa cttctccttg gtgactcccc gaatcgagtt
     2101 ggcctcgcgt cgctcccgcg cggccgcgag caggcccgcc tggatctcct cgagcgcctg
     2161 ggcggcggtg gcggcgatcc cggccagcgg cactggcgcc ttccctccgg tgcggcgcgc
     2221 ggccatcgcg tgctcggccg cgacgtcctt cggtccgatc tcgaagcgca gcggcacgcc
     2281 ccgcgcctcc cactcgaagt acttggcgcc cggcttcaag ccgtcgcgtg catcgagcac
     2341 gacgcgcagt ccggccgcct tcagctctcc ctcgacccgc gtcgcgactt ccagcacccg
     2401 ggcgcgctcc tcgtcgctct tccagatcgg caccaggacg acctgcaccg gggcgagccg
     2461 gggcgggcac accagcccgt tatcgtcgga atgggtcatg atgagcccgc cgatgagccg
     2521 ggtggtcact ccccaggacg tactccatac gtattccagt ccccccgccg ccgtctggta
     2581 ctgcacgttg aacgcgcggg cgaagttctg gcccaggttg tgtgaggtcc ccgcctgaag
     2641 cgccttgcgg tcctgcatca gcgcctcgat cgcataggtg cgcaacgccc ccgcgaactt
     2701 ctcggtgtcg gtcttgacgc ccgtcagcac cggcatcgcc aaccattctt cgacaaaggt
     2761 ccggtagatg ccgagaatca accgggcttc gcgctcggcg tcggcctcgt cggcgtgcgc
     2821 cgtatgcccc tcctgccaca ggaactccgt ggtgcgcaga aacaggcgcg tgcgcagctc
     2881 ccagcgcacg acgttgcacc actggttgag caacagcggc aggtcgcggt agctctggat
     2941 ccacttggcg aacatcgcgt agatgatcgt ctcggaggtg ggccgcacca ccagcggctc
     3001 ctccagcgcg gacgcggggt ccagctccac ggctcgcgcc ccggtcttgc cggtcgcccg
     3061 caggcgcgtg tgcgtcacga gcgcaacttc cttggcgaac ccctccacgt gctccgcttc
     3121 cttggccagg aagctcatcg gaatgaacag cgggaagtac gcgttctggt ggcccgccgc
     3181 cttgaacatg tcgtccagcg cgcgctgcat cagctcccag atgcggtagc cgttggggcg
     3241 aatcaccatg cagccgcgca ccggcgagta atcggccaat tcggcctgca tgatgacgtc
     3301 gttgtaccac tcgctgtagt ccttggcgcg tggcgtgatc ttctctgcct gggccatgtc
     3361 ccgggagcga tcctctcgga gtgtggactg gacggttaca gccgctcgag tacttccacc
     3421 gtccgctcgt tgcgcacatt gccggtattc aaagtcgtgg ccggctcaaa cagcatcacc
     3481 tcgacctcgt gctccgcgac cggccggtgc tccacgccgc gcggcacaat gaggaattcg
     3541 cccgcagcca cgtccaccga ccggtcccgg aattccatcc ggaaccttcc cttcaccacg
     3601 tagaacagct cgtcctcctg gtcgtgatga tgccagacga agtcgccgat gaacttcacg
     3661 agcttcacct gctggccgtt cagctctccg acgatcttcg gcgaccagta ctcgttcaca
     3721 cgcgcaagtt tctcggcgag gttgacctta tccatgagga ccgtccttcc cttgcaggtg
     3781 catacccagc tcggccatgg cgacccgttc ttcggttccg cgttgcagat cgcgcagcac
     3841 gacctggccg gccgcgcgct cgttgggtcc gatgatcacc gctcgcggcg ctccgcgcga
     3901 cgccgccagc tcgagctgct tgcggaccgc ctgcagccgc agtccgtact cgaccgcgag
     3961 gccgcgcccc cgcagctcat gcgccagacg caacaccgtc gccagatcct cgccgcccac
     4021 ggcgacgaga aagagatcca gccgggacga cccggcgggg accgc
//
LOCUS       DBNGZW010000500         4296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_994914, whole genome shotgun sequence.
ACCESSION   DBNGZW010000500 DBNGZW010000000
VERSION     DBNGZW010000500.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4296
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_994914"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1839
                     /locus_tag="AC16JC_11740"
     CDS             <1..1839
                     /locus_tag="AC16JC_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HIR5165411.1"
                     /translation="ARARGIPVHMPWHDLDAPQRHFLLHGGAGGGRYLGVFPFLERLE
                     SKRYKQYIRVFLRQYQLAKTCPACRGARLKPEALAVRVGGRTIAEVSALTAGELQRWL
                     GDLTWTPFQQAIARHLLEELTARVSFVVDVGLGYLTVDRQTRSLSGGEAQRIALSNAL
                     GSKLTETLYVLDEPTIGLHPGDVQRLLALLRRLATAGNTVVVVEHDPAAMAVADYMVE
                     LGPGSGTAGGSVVYQGPAAGVKSAGTLTGQYLSGEKRISVPSARRPALRWLTLRGARL
                     HNLQNVDVQIPLHALVAITGVSGSGKSTLVHDVLYRQLERRLRGGHSAKEHLGEPVGE
                     VAALEGWEAIGDVVLIDQSPIGRTPRSNPVTYVKAFDELRQLFAALPRARERRYTAST
                     FSFNVRGGRCEACEGAGAVLVEMVFLANVFVPCEACGGRRFKKEVLDVRLDGQSIHDV
                     LEWTVDEALRRFHKQPRLARALWQLQQVGLGYLRLGQPATTLSGGEAQRLKIARELTL
                     SGIRPARGGGPGGRRNLYILDEPTTGLHLDDVRTLCRVLDRLVDAGHTVVVIEHHLDV
                     IKRADWVIDLGPGAGSSGGHIVAAGTPEAVGQVAESATAHYLRAVL"
     gene            1836..2723
                     /locus_tag="AC16JC_11745"
     CDS             1836..2723
                     /locus_tag="AC16JC_11745"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5165412.1"
                     /translation="MTRRQADLALISACLLWGVSFVVVKLALENATPLAFAAVRFAIG
                     ALLLAPFARLGRRFTAAELGAGALLASLLGIGFLAQTSGLVWTTPSRSAFIVAISSVL
                     APAMAALALRERPGWPLVTAVLLAAWGTYWLTDPEAGGLNRGDVLTLATAVLFGAQIV
                     ATTALSRRFDPFRLVWLQIAGTAVLGAFGAALLERPAIHWTTTFIWALAYTTVGPTVV
                     ALLLQMVSQRHMSSARAALLFCFETLFAAATSWMVLNEHLSAQQWLGGGLILGGLMLA
                     ELKAPSAVGYQPSAERDSG"
     gene            2811..2883
                     /locus_tag="AC16JC_11750"
     tRNA            2811..2883
                     /locus_tag="AC16JC_11750"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2844..2846,aa:Asn,seq:gtt)
     gene            2966..3124
                     /locus_tag="AC16JC_11755"
     CDS             2966..3124
                     /locus_tag="AC16JC_11755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR5165413.1"
                     /translation="MKPQRVFGVPFKTLPAMQKRVGTDRALAAALWASGWYEARLLAA
                     FVARGGAR"
     gene            3156..>4296
                     /locus_tag="AC16JC_11760"
     CDS             3156..>4296
                     /locus_tag="AC16JC_11760"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR5165414.1"
                     /translation="MTLSAEPPSRNPQDALAPVLDGAFLQALLEHSSDVVVLLDAAGT
                     ILYTSPAVTPLLGYLPGELAGGNAFAMLHPDDLPRAQGLFGSLLEHPDDPVTARVRYR
                     HKDGSWREIEGVGINRLRDPVVRAIVVNYRDVTARHAAETEARRTVSLLQSTLESTAD
                     GILVVDEVGRIVSYNRRFVELWRMPQAVLDSQDDDRALAHVLDQIANPEQFLAKVREL
                     YAHPEAASFDILEFKDGRIFERFSLPQRLDGKSVGRVWSFRDVTSRARAERALRESEE
                     RYRAFITHTSEGVFRIEYQPPVPVDLRVDEQIEAQYRSGVLAECNAVLAQMYGFERPE
                     QLVGRHQYEFLDQSDPSNLEAMRRFVLAGNRDLDAEYHEFDRLGRR"
BASE COUNT          588 a         1491 c         1513 g          704 t
ORIGIN      
        1 gcgcgcgcgc gcggcatccc ggtgcacatg ccctggcacg acctcgatgc ccctcagcgt
       61 cacttcctgc tgcacggcgg cgccggcggc ggccgctacc tgggagtctt cccgttcctc
      121 gagcggctcg agtccaaacg ctacaagcag tacatccgcg tcttcctccg gcagtatcag
      181 ctggcgaaga cctgtcccgc ctgccgcggg gcccgcctca agcccgaagc cctggccgtc
      241 cgggtgggcg ggcgcacgat cgccgaggtc agcgccctca cggccgggga gctgcagcgc
      301 tggctggggg acctcacctg gacgccgttc cagcaggcga tcgcccggca cctgctcgag
      361 gagctgacgg cgcgagtgtc gttcgtcgta gacgtgggcc tcgggtacct gaccgtcgat
      421 cgccagaccc gttccctgtc gggcggtgaa gcccagcgca tcgccctctc gaatgccctc
      481 ggctcgaagc tcacggagac cctgtatgtc ctcgacgagc cgaccatcgg cctgcacccg
      541 ggcgacgtgc aacgcctgct cgccctgttg cgtcgtctcg cgaccgccgg caacacggtc
      601 gtcgtcgtgg agcacgaccc ggccgccatg gccgtcgcgg actacatggt ggagctgggt
      661 cccgggagcg ggacggcggg gggctcggtc gtctatcagg gaccggccgc cggggtgaaa
      721 tcggccggta ccctcaccgg ccagtatctg tcgggggaga agcggatcag tgtgccgtcg
      781 gcgcggcgcc ccgccctgcg ctggctcaca ctgcgcgggg cccggttgca caacctccag
      841 aacgtggacg tccaaattcc cctgcacgcg ctcgtcgcca ttaccggcgt gtcgggctcc
      901 ggcaagtcca ccctggtgca cgacgtcctg taccgccagc tggagcgccg gctccgcggc
      961 gggcacagcg ccaaggagca cctgggcgag ccggtgggcg aggtcgccgc gctggagggc
     1021 tgggaggcca tcggcgacgt cgtcctgatc gaccaatcgc cgatcggccg cacgccgcgg
     1081 tccaacccag tcacttacgt gaaggcgttc gacgagctgc gacagctgtt cgccgccctg
     1141 ccccgcgccc gggagcggcg ctacaccgcg agcacgttct ccttcaatgt ccggggcggg
     1201 cgctgcgagg cgtgcgaggg agccggggcg gtgctcgtgg agatggtctt cctggcgaac
     1261 gtgttcgtcc cgtgcgaggc gtgcggcggg cggcgcttca agaaggaggt gctcgacgtc
     1321 cggctcgacg gccagtcgat tcacgacgtg ctcgagtgga cggtggacga agcgctgcgc
     1381 cgcttccaca agcagccgcg tctggcccgg gcgttgtggc agctgcagca ggtgggactc
     1441 gggtatctgc ggctggggca gccggccact acgctctcgg gcggagaagc ccagcggctc
     1501 aagattgcgc gcgagctgac gctgtcgggg atccggcccg ctcggggggg cggcccaggc
     1561 ggccggcgca acctctacat cctggacgag ccgaccaccg ggctccacct ggacgatgtg
     1621 cggacgctgt gccgcgtgct cgaccgcctg gtggacgccg gacacaccgt ggtggtcatc
     1681 gagcaccacc tggacgtgat caagcgggcg gactgggtga tcgacctggg tccgggggca
     1741 ggcagttcgg gagggcacat cgtcgcggcc ggcacgccgg aggccgtggg tcaggtcgcg
     1801 gagagcgcca ccgcccacta tctgcgagcg gtactgtgac gcgccgccag gccgacctcg
     1861 cgttgatctc cgcctgcctg ctttggggag tcagcttcgt cgtcgtgaag ctcgctctgg
     1921 agaacgccac gccgctggcg tttgccgcgg tgcgcttcgc gatcggcgcc ctgctcctgg
     1981 cgcccttcgc gcggctgggc cggcgcttca cggccgcaga gctgggagcc ggcgccctgc
     2041 tcgcgagtct gctcggcatc ggctttctcg cccagacctc cggtctcgtg tggacgacgc
     2101 cttctcgatc cgccttcatc gtcgccatct cgtcggtgct cgcgccggcc atggcggcgc
     2161 tggcgctgcg cgagcggcca ggttggccgc tcgtcacggc ggtgctcctc gccgcatggg
     2221 gcacctactg gctgaccgat cccgaggcgg gcgggctcaa tcgcggcgac gtgctcaccc
     2281 tcgcgacggc ggtgctgttc ggtgcccaga tcgtggcgac caccgccctg tcccgtcgct
     2341 tcgacccgtt tcgcctcgtg tggctgcaga tcgcgggaac cgcggtgttg ggcgcgttcg
     2401 gggctgccct gctcgagcga cccgcgatcc actggacgac cacgttcatc tgggccctgg
     2461 cttacaccac ggttggcccg accgtggtcg cgttgctgct gcagatggtc tcgcagcggc
     2521 acatgtcatc ggcgcgcgcc gcgctgctct tctgcttcga gacgctgttc gccgcggcta
     2581 cgtcgtggat ggtgctgaac gagcacctgt cggcccagca gtggttgggc ggcgggctga
     2641 tcctcggcgg actgatgctg gcagaactga aggcaccgtc ggctgtcggc tatcagccat
     2701 cggcagagcg cgattcgggt tgaagtcgcg gcgcctggtg gcgatattac caccccacac
     2761 acgggtagcg gatagcagat agctggtagc cgttggcctt cagcatccag tcccgagtag
     2821 ctcagttggt agagcagccg gctgttaacc ggcgggtcgg gggttcgagt ccctcctcgg
     2881 gagcttctcc tacaccgcca ccgactcgag gtggacgccc gccacagcgg agccccgcgg
     2941 gggtaatcgc ggggcctcag cttcgatgaa gccccagcgc gtgttcggcg tgccgttcaa
     3001 gaccctgccg gcgatgcaga agcgggtggg gacggatcgc gccctggccg cggcgttgtg
     3061 ggccagcggg tggtacgagg cgcgattgct ggcggcattc gtggcgcgcg ggggggcgcg
     3121 gtagcgcttt tgtcgccgtc ttgcagattt atgccgtgac gctttccgcc gaaccgccgt
     3181 cccggaatcc ccaggacgca ctcgcaccgg tcctggacgg cgccttcctc caggcgctgc
     3241 tggagcacag ctccgacgtc gtcgtcctgc tcgacgcagc cggtacgatc ctctacacct
     3301 caccggccgt gactcccctg ctcggctacc tgcccgggga gctggcgggc ggcaacgcat
     3361 tcgcgatgct gcaccctgac gatctgccgc gggcgcaggg cctgtttggg agcctgctgg
     3421 agcatcccga cgatccggtc acggcgcggg tccggtaccg ccacaaggac ggcagctggc
     3481 gcgagatcga gggggtgggg atcaaccgcc tccgcgaccc cgtggtgcgc gccatcgtcg
     3541 tgaactaccg cgacgtcacc gcccgccacg cggcggagac cgaagcccgc cgcaccgtct
     3601 cattgctgca gtccacgctc gagtcgacgg ccgacggcat cctcgtggtg gacgaggtgg
     3661 gccggatcgt ttcctacaac cgccgcttcg tcgagctgtg gcgcatgccg caggcggtcc
     3721 tcgattccca ggacgacgat cgcgcgctcg cccacgtgct cgaccagatc gccaacccgg
     3781 aacagttcct cgccaaggtc cgggagctct acgcgcatcc cgaggcggcg agcttcgaca
     3841 tcctcgaatt caaggatggg cgcatcttcg agcggttctc cctgccccag cgcctcgacg
     3901 gcaagtcggt cggacgggtc tggagcttcc gcgacgtgac ctcccgggcg cgcgccgagc
     3961 gcgccctgcg ggagagcgag gagcggtacc gggccttcat cacgcatacg tcggaagggg
     4021 tgtttcgcat cgagtaccag ccgcccgtgc cggtggacct ccgcgtggac gagcagatcg
     4081 aggcccaata ccggtccggc gtgttggccg agtgcaatgc ggtgctggcc cagatgtacg
     4141 gcttcgagcg gcccgagcag ctggtcggcc ggcaccaata cgagttcctc gaccagtcgg
     4201 acccgtcgaa tctcgaagcg atgcggcggt tcgtcctggc gggaaatcgc gatctggatg
     4261 ccgaatacca cgaattcgac cggctggggc gccgcc
//
LOCUS       DBNGZW010000501         3968 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1615964, whole genome shotgun sequence.
ACCESSION   DBNGZW010000501 DBNGZW010000000
VERSION     DBNGZW010000501.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3968)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3968)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3968
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1615964"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="AC16JC_11765"
     CDS             complement(<1..365)
                     /locus_tag="AC16JC_11765"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5165415.1"
                     /translation="MPFVTFELERWQSTWENRVRFNLSESGVHPLSVRELLELAGSPA
                     DPLLDVRLGYSQSNGTDALRERIAALYPGATPDQVLVTTGSAEANYLAALRLIEPGDT
                     VAMLLPNYLQLWGHAQNLGA"
     gene            complement(382..1020)
                     /locus_tag="AC16JC_11770"
     CDS             complement(382..1020)
                     /locus_tag="AC16JC_11770"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HIR5165416.1"
                     /translation="MPFSTLLFDLDGTLIDSIDLILRSYRHTMMVHRGAEPPDDVWME
                     GLGTPLSVQFRRWTDDEAEIRAMVETYRTFNLAHHDELVRAYDGVVEQVLRLDAGGKT
                     LGLVTSKMRGGALKGLRLVGIEAAFDVVVGSDEVTHPKPHPEPVLLALERLGRAPADA
                     VFIGDSRHDIECGRAAGVKTAAVLWGPFDREHLEDLDPDYWLERPSDLSTLA"
     gene            complement(1027..1710)
                     /locus_tag="AC16JC_11775"
     CDS             complement(1027..1710)
                     /locus_tag="AC16JC_11775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165417.1"
                     /translation="MRHPSLPLRAYIDRLRTLYGRPKRPIPSGPFEWIIWEHVAAVPD
                     DEQRAIVFRTLRQKIGTTPQAVLRTPKPKMLELLAPGGPNAAARLSQLREAAELMIEI
                     GLARVRTLVRESPAEAKKLLKRFKGIGETGADRILLFCRTQAVLAPEANGLRVLTRLG
                     YGKGAGAAAYKQVSEALAPDLPRDYNWLIQAHQLLRRHGQDICRTTPRCDACPLAPDC
                     AWYHQHGGG"
     gene            complement(1746..>3968)
                     /locus_tag="AC16JC_11780"
     CDS             complement(1746..>3968)
                     /locus_tag="AC16JC_11780"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012493.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5165418.1"
                     /translation="AAALALLDLPGRSSPAPDWLQPHQVPAHTRLLAIMARYGGAVLA
                     DAVGLGKSYVALAVAKSLGAPLVVVAPAVLVPQWRALMDRLGVAGRLVTHESLSRRAP
                     PDVPARRMVRDTAPLLIVDEAHRFREPGTLRYRALARLALRARVLLVTATPVHNRPAD
                     LLHLLRLFLRDDALVGLGVPSLTAAARATASAPLALPALARIVVARSRRRVVQAWAGL
                     SFPRRDAATVIRAASVEPHLVSSIAESLRSLQPPGGAAALVRLTLLRQFASSLPALTH
                     SLRRYQAFCELRLEAVRTHRRLGSREFRRLFPPGDESCLQLAFLPLLLDEGHPASDGG
                     DDLETVRRLLQRLGPGPDPKADALERLLTERPARTIVFTTAAATVHHLRRRLMRKVRV
                     GAVTGHSGWLAGDRVSRHEVIAAFAPRAQHAPQPAAACAVDLLIATDLLSEGLDLQDA
                     QRVVHYDLPWSPARVAQRVGRIDRLASPHPDIHTIAFLPPGPLARELALEHRLALKVS
                     AQLGAGAAQVEHVSGVGRDDAPLDWCDRLQRLAGEDGTVVPPGAVAAVASGVAACVLV
                     LRLGTDVEAIVVEDGRAAADAVRATALLEQSQCAAPRPLDQGALSAAVRAALPVLRVR
                     LEAIARARWRAADRDHIGRRLVPLALAAARRAARSGRPERLARLDALVSRLTGGQTAG
                     EALSLEALVSQREPLTAQILLDWHEALPPLEVVTEPPQPELVAAVVLGDQGAGPCAPR
                     "
BASE COUNT          625 a         1359 c         1461 g          523 t
ORIGIN      
        1 gcgcccaggt tctgcgcgtg tccccagagc tgcaggtagt tgggcagcag catggcgacg
       61 gtgtcgcccg gctcgatcag ccgcagcgcc gcgagatagt tggcctccgc actgccggtc
      121 gtgaccagca cctggtccgg cgtcgcgccg ggatacagcg cggcgatccg ttcgcgcagc
      181 gcgtcggtac cgttcgactg gctgtagccc aaccggacgt cgagcagcgg gtcggccgga
      241 ctgcccgcca gctcgagcag ctcgcgcacg ctgagcgggt gcacgcccga ctcggagaga
      301 ttgaagcgca cccggttctc ccaggtgctc tgccagcgtt cgagctcgaa agtgacgaag
      361 ggcacggatg gctcccggga atcaggccag cgtggagaga tccgacggcc gctccagcca
      421 gtagtcggga tcgagatctt cgagatgctc gcggtcgaac ggcccccaca acaccgcggc
      481 ggtcttcacg ccggcggcac ggccacactc gatgtcgtga cgcgaatcgc cgatgaacac
      541 cgcatcggcc ggcgcgcgcc cgagccgttc gagcgcgagc aacaccggct cgggatgcgg
      601 cttgggatgg gtgacctcgt cggatccgac gaccacgtcg aacgccgcct cgatcccgac
      661 caggcgcagt cccttgaggg cgccgccgcg catcttgctc gtgaccaggc cgagcgtctt
      721 gccgccggcg tcgaggcgga gcacctgctc gacgacgccg tcatacgccc gcaccagctc
      781 gtcgtgatgc gcgaggttga aggtgcggta cgtctccacc atcgcacgaa tctcggcttc
      841 gtcgtccgtc cagcggcgga actgcaccga caggggtgtg cccagacctt ccatccagac
      901 gtcgtcgggc ggctccgcgc cacggtggac catcatcgtg tggcggtagg agcggagaat
      961 cagatcgatc gaatcaatca gcgtcccgtc gagatcgaac aacagcgtcg agaacggcac
     1021 ccgcgctcac ccgcccccgt gctggtggta ccaggcgcag tccggagcga gcgggcaggc
     1081 gtcgcagcgg ggtgtcgtgc ggcagatgtc ctggccgtga cgccgcagca gctggtgggc
     1141 ctggatgagc cagttgtagt cgcgcggcag gtcgggcgcg agcgcctcgc tcacctgttt
     1201 gtaggccgcc gccccggcgc ccttgccgta tccgaggcgc gtgaggacgc ggaggccgtt
     1261 cgcctcgggg gcgagcaccg cctgagtgcg gcagaacagc agaatccgat ccgctcccgt
     1321 ttcgccgatt cccttgaagc gcttgagcag cttcttcgcc tccgccgggc tttcgcgcac
     1381 cagggtccgg acgcgggcga gcccgatctc gatcatcagc tcggccgcct cccgcagctg
     1441 cgacagccgg gccgcggcat tgggcccgcc cggcgcgagc agctcgagca tttttggttt
     1501 cggggtccgc aggacggcct ggggcgtcgt gccgatcttc tgacgcagcg tgcggaacac
     1561 gatggcccgc tgctcatcgt cggggacggc ggcgacgtgc tcccagatga tccactcgaa
     1621 gggaccactc ggaatgggcc gcttggggcg gccgtagaga gtacgaagac ggtcgatgta
     1681 ggcccggagg ggcagggagg ggtgtcgcat cggacactaa aattgcctcc ggaggagcct
     1741 tggcactagc ggggtgcaca ggggcccgcc ccttgatcgc ccaggacgac ggcggccacg
     1801 agctccggtt ggggtggttc ggtgaccacc tcgagtggcg gcagggcctc gtgccagtcg
     1861 agaaggatct gggccgtcag gggttcgcgc tgcgagacga gcgcttcgag cgagagcgcc
     1921 tctcccgccg tctgaccccc ggtcagtcgt gaaaccaggg cgtcgagccg ggccagccgc
     1981 tcgggacgcc cgctgcgagc agcccgccgc gcggcggcga gcgccagcgg cacgagacgg
     2041 cggccgatgt ggtctcggtc ggcggcgcgc cagcgggccc tggcgatcgc ctcgagccgt
     2101 acgcgcagca ctgggagcgc cgcacggacc gcggcggaca gggctccctg gtcgagcggc
     2161 cggggtgctg cgcactgcga ttgctccagc agggcggtag ctcgcacggc gtcggccgca
     2221 gcccggccat cctcgaccac gatcgcctcg acatccgtcc cgaggcggag cacaagcaca
     2281 caggcggcga caccggaggc gaccgccgcc accgctcctg gcggaacaac cgtcccgtcc
     2341 tccccggcga gccgctgcag gcgatcgcac caatcgagcg gcgcgtcgtc gcgacccacg
     2401 ccggaaacgt gctccacctg tgcggctcca gcgccgagct gcgccgaaac cttcagcgcg
     2461 aggcgatgct ccagggcgag ctccctggcc agcggtccgg gagggaggaa ggcgatcgtg
     2521 tgaatgtccg gatgcggcga tgccagccgg tcgatccgcc cgacgcgttg cgccacacgg
     2581 gcggggctcc aggggagatc gtagtggacg acgcgctggg catcctgcag atcgagcccc
     2641 tcgctgagca ggtcggtcgc gatcagcagg tcgaccgcgc acgcggcggc cggctgcggc
     2701 gcgtgctggg cgcggggagc gaacgcggcg atcacctcgt ggcgggagac ccgatcgccg
     2761 gccaaccagc cggagtgtcc cgtcacggcc cctacccgga ccttcctcat caggcggcgt
     2821 cgcaggtgat gcaccgtggc cgcggcggtg gtgaagacga tcgtccgggc gggccgctca
     2881 gtgagcaacc gctcgagcgc atcggccttc gggtcggggc ccggaccgag gcgctggagg
     2941 agccggcgca ccgtctccag atcgtcgcca ccgtcggatg ccgggtggcc ctcgtcgagc
     3001 agcagcggaa ggaatgcgag ctggagacag gactcatcgc cggggggaaa cagtcggcgg
     3061 aactcacggc ttcccagacg acgatgcgtg cgcaccgcct cgaggcgcaa ttcgcagaac
     3121 gcttggtagc ggcgcagact gtgagtcaag gccgggagac tcgatgcgaa ttgccgcagc
     3181 agtgtgagcc ggaccagggc cgcggcgccc ccgggcggct gcagcgaccg cagcgactcc
     3241 gcgatggagg aaaccaggtg tggctccacg ctcgcggcgc ggatcacggt ggcggcgtct
     3301 cgtctgggga acgacagtcc cgcccaggcc tgtacgactc ggcgacgcga gcgcgcgacc
     3361 acgatgcgcg cgagggccgg cagggccaac ggtgccgacg cagtagcccg cgcggcagcg
     3421 gtcagggagg gaacgcccag cccgaccaga gcgtcgtcgc gcaggaacag acgcaacagg
     3481 tgcagcaagt ccgccggccg gttgtggacc ggcgtggcgg tcacgagcag cacacgcgct
     3541 cgcagggcga gccgggccag cgcgcgatag cgaagcgtgc ccggctcccg aaagcggtgt
     3601 gcctcgtcga cgatcaggag cggcgccgtg tcccgcacca tgcgccgggc cgggacgtcc
     3661 ggcggtgctc gacggctgag gctctcgtgc gtgacgaggc ggcctgcgac gccgagccgg
     3721 tccatcagtg cgcgccattg cggcacgagc acggccggcg cgacgacgac caggggagcg
     3781 ccgagcgatt tcgcgacggc caaggcaacg taggactttc ccaatcctac cgcgtccgcc
     3841 agcaccgcac ccccgtagcg ggccatgatg gcgagcagcc tggtgtgggc ggggacctgg
     3901 tgcggctgga gccagtccgg cgcgggactg gaacgtccgg gaaggtcgag cagcgcgagg
     3961 gccgcagc
//
LOCUS       DBNGZW010000502         4094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1547936, whole genome shotgun sequence.
ACCESSION   DBNGZW010000502 DBNGZW010000000
VERSION     DBNGZW010000502.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4094
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1547936"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="AC16JC_11785"
     CDS             <1..417
                     /locus_tag="AC16JC_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5165419.1"
                     /translation="DPRAVNPATTMPNQRLAAGDVTALVSFFRWVGGINTNDWPPKPR
                     LSLGGGADTASGTVVFQQKGCSACHRIAGTGAVGPGPDLPRIGATPYDGLPNTPEFLA
                     KWLENPQAQKPGTVMPRLPLTPTERDALVRYLTSLK"
     gene            430..1791
                     /locus_tag="AC16JC_11790"
     CDS             430..1791
                     /locus_tag="AC16JC_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR5165420.1"
                     /translation="MTTVPQLRHPSQRVAYAFFVAAAILFLLQVLFGLLIAAQYVWPT
                     FLMNELPFNVGRATHLNLLTFWTLLGLMGATYYLVPDETDSELASVPLAYVQLGVMLL
                     AGVGALVSLWFFGQSRGKPFTEAAMPWPWVIALGAVLFLVNVGATIVRARRWTAIAVV
                     LFGGMTGLSLLYLINLGTSANLAVDYYWWWWLIHLWVEGTWELVAAAIMAYLLITLTG
                     VERARMHRWLYVEVSLVLFTGIIGIGHHYYWIGTPRYWLLWGALFSAFEPIPIALMAW
                     DAFASMKHRRAEPTNRVAWYWLGGSAIGHLLGAGVWGFAMTLPQINKWTHGTQLTASH
                     GHFAFYGAFAMLVLAAVYTILPRLRGIARVPERRGLWAFWLMTAGMLAMVTAFTIAGV
                     VQVYLQRMLGLDFMTVRTEYVGFWIFWVWLFGLSLFLPGCALYVRDFFALTRKTSLTP
                     LPT"
     gene            1858..3144
                     /locus_tag="AC16JC_11795"
     CDS             1858..3144
                     /locus_tag="AC16JC_11795"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008601095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HIR5165421.1"
                     /translation="MPLHFLAVAALAFTVPAPQQADGASPVPAAAQASDHFDPVAATR
                     AYLATVPAEKKARSDAYFEGGYWLILWDFLLSAGVYLLLLVTGWSARMRDRAERVTRF
                     APLRTALYWVQFLIVTSVLVFPLTVYAGFVREHAYGLATQGFGSWFGDQLKGLAVGLV
                     FGGVFAIVIYAVLRRAQRTWWLWGSVVTVLFQMLGALIAPVYIFPLFNTYTKLDDPRV
                     TAPILSMARANGIPASEVYEVNASRQSTRVSANVSGFLGTERITLNDNLLRRASLPEI
                     EAVMGHEMGHYVLNHVYKGILFFGIVVVVGFALLRWGAGRVLERWGAAWGLRGVDDVA
                     ALPLLALLVTIYGFLLTPITNSYVRTQEYEADIFGLNAAREPDGAATIALKLGEYRKL
                     DPGALEEVLFFDHPSGRTRIMAAMRWKAEHLGNGGT"
     gene            3298..3723
                     /locus_tag="AC16JC_11800"
     CDS             3298..3723
                     /locus_tag="AC16JC_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165422.1"
                     /translation="MRRTFLGGLSAALGLGLLFTAPQTAAAQRTAPPGGSYKQVSTLV
                     PLPDFVPGLGTLYVDPATLPAGPFLAYDRKGTLVSSIYMIPLSQMNAQKNFADLNVAK
                     GQAVDHVDIVYNAGHPGVAEPHYHVILWYVPAATAAALQ"
     gene            3730..>4094
                     /locus_tag="AC16JC_11805"
     CDS             3730..>4094
                     /locus_tag="AC16JC_11805"
                     /inference="COORDINATES: protein motif:HMM:NF012355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HIR5165423.1"
                     /translation="MTTRRGFVQAGGVFLAGLAVRPWWRLRPSLATTVEIAMRSDAAG
                     AKVWFDPAGVLVQPGTTIRWVVRENVHTTTAYHPRNGGHSLRIPEGAAPWDSGFLVNP
                     GDHFEVTLTVLGVYDFFCMP"
BASE COUNT          575 a         1392 c         1369 g          758 t
ORIGIN      
        1 gatccgcgcg ccgtcaatcc cgcgacgacg atgcccaacc agcggctcgc cgcgggcgat
       61 gtgactgcgc tcgtgtcgtt cttccggtgg gtgggcggca tcaacacgaa cgattggccc
      121 cccaagccgc gtctcagcct cggcggcggc gccgataccg cgagcggtac ggtggtcttc
      181 cagcagaagg ggtgctccgc ctgccatcgg atcgccggga cgggcgccgt cggaccgggc
      241 cccgacctgc cccgcatcgg cgctaccccc tatgacgggc tgcccaacac gccggagttc
      301 ctggcgaagt ggctcgagaa cccgcaggcg cagaagcccg gtacggtgat gccgcggctc
      361 ccgctgaccc cgaccgagcg cgacgccctc gtccgctacc tcacgtcgct caagtagagg
      421 gctccgaccg tgaccactgt tccgcagctc cggcacccgt cgcagcgggt cgcctacgcg
      481 ttcttcgtcg ccgcggcgat cctgttcctg ctgcaggtgc tgttcggcct gctcatcgcc
      541 gcccagtacg tctggccgac gttcctgatg aacgagctcc cgttcaacgt gggccgggcg
      601 acgcacctca atctgctgac cttctggaca ttgctcggcc tgatgggggc gacctactac
      661 ctcgtgcccg acgaaaccga ctccgagctg gccagcgtcc cgctggccta cgtgcaactg
      721 ggggtgatgc tgctcgccgg cgtcggtgcg ctcgtcagcc tctggttctt cggccagagc
      781 cgggggaagc cgttcaccga ggcggcgatg ccctggccct gggtgattgc gctgggggcg
      841 gtgctgtttc tcgtcaacgt cggggccacc atcgtgcggg cgcggcgctg gaccgcgatc
      901 gccgtcgtgc tgttcggcgg catgacgggc ctttccctgc tgtacctgat caacctcggt
      961 acctccgcca acctggccgt ggactactac tggtggtggt ggctcatcca cctctgggtc
     1021 gagggaacct gggagctcgt cgccgcggcg atcatggcgt acctgctgat cacgctgacc
     1081 ggcgtcgagc gggcccggat gcaccgctgg ctctacgtcg aggtgtcgct ggtgctgttc
     1141 accgggatca tcgggatcgg ccaccactac tactggatcg gcactccccg ctactggctg
     1201 ctgtggggcg cgctgttcag cgcgttcgag cccattccca tcgccctgat ggcgtgggat
     1261 gcgttcgcga gcatgaagca tcgccgggcg gagcccacga accgcgtcgc ctggtactgg
     1321 ctgggcggct cggcgatcgg ccacctgctg ggcgcgggcg tgtggggctt cgccatgacg
     1381 ctcccgcaga tcaacaagtg gacgcacggc actcagctga ccgcgtccca cggccacttc
     1441 gctttctacg gagcgttcgc gatgctcgtg ctcgccgcgg tctacaccat cctcccgcgg
     1501 ctgcgaggga tcgcgcgcgt gccggagcgc cgggggctct gggccttctg gctcatgacg
     1561 gcgggtatgc tcgctatggt gacggcgttc accatcgccg gagtggtgca ggtctacctg
     1621 cagcgcatgc tgggcctcga cttcatgacg gtccgcacgg aatacgtggg cttctggatc
     1681 ttctgggtct ggctgttcgg gttgtcgctg tttctccccg gctgcgcgct gtacgtgagg
     1741 gacttcttcg ccctgacgcg gaagacgagt ctcactccgt tgccgaccta ggagccccgg
     1801 caccgcagct ccccgggtcg ccttgcccca tggccgccgg gaggtgcatg ctatcgcatg
     1861 cccctccact tcctcgcggt cgccgccctc gccttcacgg tgccggcgcc gcagcaggcc
     1921 gacggcgcgt cgcccgtgcc ggccgcggcg caggcgagcg accacttcga tccggtcgcg
     1981 gcaactcgcg cctacctcgc cacggttccc gccgagaaga aggcgcgctc cgatgcctac
     2041 ttcgagggtg ggtactggct gatactgtgg gacttccttc tgtcggccgg cgtgtatctg
     2101 ctgttgctcg tcaccggatg gtcggcccgg atgcgcgatc gcgccgagcg ggtcacgcgt
     2161 tttgctcctt tgcgtacggc gctctactgg gtgcagttcc tgatcgtgac cagcgtgctc
     2221 gtcttcccct tgaccgtgta cgcggggttc gtgcgcgagc atgcgtatgg gctcgccacc
     2281 cagggcttcg gttcgtggtt cggcgatcaa ttgaagggac tggcggtggg gctcgtgttc
     2341 ggcggcgtgt tcgcgatcgt gatctacgcc gtactgcggc gcgcgcagcg cacctggtgg
     2401 ctctggggca gcgtggtcac cgtgctgttc cagatgctcg gcgccctgat cgcgccggtc
     2461 tacatctttc cgctcttcaa tacctacacg aaactcgacg acccacgcgt gacggcaccg
     2521 atcctcagca tggcgcgcgc caacgggatc cccgccagcg aggtgtacga agtgaacgcc
     2581 tcgcggcagt cgacgcgcgt cagcgccaat gtctcggggt tcctcggcac cgagcgcatc
     2641 accctgaacg acaatctgct gcggcgcgcc tcgctccccg aaatcgaggc cgtcatgggg
     2701 catgagatgg gacactacgt gctgaaccac gtgtacaagg gcatcctctt cttcgggatc
     2761 gtggtcgtag tgggcttcgc gctgctgcgc tggggcgcgg gccgggtgct cgagcgctgg
     2821 ggggccgcgt ggggcctgcg cggcgtggac gacgtcgcgg cattgccgct gctcgcgctg
     2881 ctcgtcacga tctacggttt cctgctcacg ccgatcacga acagctacgt gcggacgcag
     2941 gaatatgaag ccgacatctt cggcctgaac gcggcgcggg aaccggacgg cgcggccacg
     3001 atcgccctga aactggggga gtaccgcaag ctcgatcccg gcgcactcga ggaagtgctg
     3061 tttttcgatc atcccagcgg ccgcacgagg atcatggcgg cgatgcggtg gaaagcggag
     3121 cacctgggga acgggggcac atgattctcc gcccaccgtc acaccggtgt gggtcgttcc
     3181 ctgaaggccg ctcgcacatc cacccggggt gtcgatgtcg tctctgctgt ggagcggcgg
     3241 gcaatgcctc gacgctcacg aatcgagcaa tcgcactcac acggagacgc acacgacatg
     3301 agacgcacct tcctcggggg cctgagcgcc gcgctcggcc ttggactcct attcaccgca
     3361 ccacagaccg ccgcggccca gcgcacggct cctccgggcg gctcctataa gcaggtcagc
     3421 acactcgttc cgctgcctga tttcgtaccg gggctgggga ccttgtacgt cgatcccgcg
     3481 acgttgccgg cgggtccgtt cctggcatac gaccggaagg ggaccctggt gagctcgatc
     3541 tacatgatcc cgctcagcca gatgaacgcc cagaagaact tcgcggatct gaacgtcgcg
     3601 aaggggcagg cggtggatca tgtggacatc gtctacaacg cggggcatcc cggcgtcgcc
     3661 gagccgcatt accacgtcat cctgtggtat gtcccggccg cgactgcggc cgcgttgcag
     3721 tagcctgaag tgacgacacg gcgcgggttc gtccaggccg gcggggtatt cctcgccggt
     3781 ctggccgtgc gcccatggtg gcggttgcgt ccctcgctcg cgaccacggt cgagatcgcg
     3841 atgcgcagtg atgcggcggg cgccaaggtg tggttcgatc cggcaggggt gctggtgcag
     3901 cccggcacca ccatccgctg ggtggtccgg gagaacgtcc acacgacgac cgcctatcat
     3961 ccacgcaacg gcggtcactc cctgcgcatt cccgagggag cggcaccgtg ggactccgga
     4021 ttcctcgtga acccggggga tcacttcgag gtgacgctga ccgtgctggg ggtctacgac
     4081 ttcttctgca tgcc
//
LOCUS       DBNGZW010000503         1847 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_526437, whole genome shotgun sequence.
ACCESSION   DBNGZW010000503 DBNGZW010000000
VERSION     DBNGZW010000503.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_526437"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1155
                     /locus_tag="AC16JC_11810"
     CDS             <1..1155
                     /locus_tag="AC16JC_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_212529335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HIR5165424.1"
                     /translation="EVEEYLGRHRGERDAAGHAVVVRNGTARPRTVTTGVGPIPVAAP
                     RVNDRRRVEGQRQRFTSAILPPYLRRSPRLAGVLPLLYLHGLSSGDFRDALPALLGPE
                     AAGLAPRTITRLTKSWTAEYDAFRRRDLADRDYVYLWADGIHFTIRLEDERLCALVLL
                     GVRPDGTKEVVALEDGYRESAESWRAVLRDLKRRGMRAPGLAIGDGALGFWAAVREVW
                     PETAEQRCWVHRIANVLDKLPTALQPRAKQALHAMLYAETRTACEREIRRFTTEYGAK
                     YPKAVAALTTDQARLLTLFDYPAEHWKHLRTTNTIESTFATVRLRERVTKGAGSRTAG
                     LTMAFKLLEAAQRHWRRLDGPALIPLVRAGVRFIDGVRVERQEERKKGAA"
     gene            1189..>1847
                     /locus_tag="AC16JC_11815"
     CDS             1189..>1847
                     /locus_tag="AC16JC_11815"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR5165425.1"
                     /translation="MSNCLHSDIRRIYVLTEYQSESLKKHISNTYKFDIFSSGFVAVV
                     AAEQIEERSDWFQGKADAVRRSVRHVGIEQWDDIVILSGDQLYRMDFLEMLRTHRESR
                     ADATIATKSVPPEQTRDFGIMRVDKAGRIVHFDEKPPVERLAGLESWLPGRKEPAYLA
                     SMGIYIFRRPALEAALSNTEHDDFGRHVIPAAVPRLHVQAHVHDGYWEDVGTIDSYFQ
                     A"
BASE COUNT          304 a          606 c          655 g          282 t
ORIGIN      
        1 gaagtcgaag agtacctcgg ccgccatcgc ggggagcgcg atgcggccgg ccacgcggtg
       61 gtcgtgcgca atgggacggc gcgcccgcgg acggtcacga cgggcgtcgg gccgattccg
      121 gtggcggcgc ctcgcgtgaa tgatcggcgg cgggtcgagg ggcagcggca gcggttcacg
      181 agcgcgatcc tgccgccgta cctgcgtcgc tcgccccggt tggccggggt cctgcccttg
      241 ctgtacctgc acgggctgtc ctcgggggat tttcgggacg cgctgccggc cctgctcggg
      301 ccggaggccg ccgggctcgc gccccgcacc atcacccggc tgaccaagag ctggacggcc
      361 gagtatgacg cctttcggcg acgcgacctc gcagaccgcg actacgtgta cctctgggcc
      421 gacggcatcc acttcacgat ccgcctggaa gatgagcggt tgtgcgccct cgtgctcctc
      481 ggcgtccggc cggacggcac caaggaagtg gtcgcgctgg aggacggcta ccgggagagc
      541 gccgagagct ggcgggcggt gctgcgggat ctgaagcgcc gcggcatgcg cgccccgggc
      601 ctcgcgatcg gcgacggcgc cctcggcttc tgggccgcgg tgcgcgaggt gtggccggag
      661 accgccgagc agcgatgctg ggtgcatcgg atcgccaacg tcctggacaa gctgcccacg
      721 gcgctccagc cgcgggccaa acaggcgctg catgcgatgc tgtacgccga gacgcggacg
      781 gcctgcgagc gcgagatccg ccggttcacg acggagtacg gcgccaaata cccgaaggcc
      841 gtggccgcgc tcacgacgga ccaggcgcgg ctcttgaccc tcttcgatta cccggcggaa
      901 cactggaagc acctgcgcac cacgaacacg atcgagtcca cgttcgcgac ggtccggttg
      961 cgcgagcgcg tgacgaaggg cgcgggctcg cggacggcgg gcctgaccat ggcgttcaag
     1021 ttgctcgaag cggcgcagcg ccattggcgg cggctcgacg gccctgcatt gatcccactg
     1081 gtacgagcgg gtgtgcggtt catcgacggg gtacgcgtcg agcggcagga ggagcggaag
     1141 aagggcgccg cctgatctcc ctcaatccac aactcttgac gatagctcgt gagcaactgt
     1201 ctgcactcgg acattcggcg catctacgtg ctgaccgaat accagtccga gagcctgaag
     1261 aagcacatct ccaatactta caagttcgat atcttctcgt cgggcttcgt ggcggtggtg
     1321 gcggcagagc agatcgagga gcgctctgac tggtttcaag gtaaggccga cgcggttcgc
     1381 cgcagcgtgc gacacgtcgg catcgagcag tgggacgaca tcgtgatcct gtccggcgat
     1441 cagctctacc ggatggactt tctcgagatg ctgagaaccc accgcgagtc gcgcgccgac
     1501 gccaccattg ccacgaagag cgtgccgccg gagcagacca gggacttcgg gatcatgcgc
     1561 gtcgacaagg ccgggcgcat cgtccatttc gacgagaagc cgcccgtgga gcggctggcc
     1621 gggcttgagt catggctgcc cgggcgcaag gagccggcct atctcgcctc gatgggcatc
     1681 tatattttcc ggcggccggc cctggaggcg gcgctatcga acacggagca cgacgacttc
     1741 ggccgtcacg taatcccagc agcggtgccg cggctgcacg tgcaggccca cgtgcacgac
     1801 gggtactggg aagacgtcgg caccatcgac tcgtacttcc aggccaa
//
LOCUS       DBNGZW010000504         5244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_799929, whole genome shotgun sequence.
ACCESSION   DBNGZW010000504 DBNGZW010000000
VERSION     DBNGZW010000504.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5244
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_799929"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="AC16JC_11820"
     CDS             <1..931
                     /locus_tag="AC16JC_11820"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR5165426.1"
                     /translation="TQRSRDLERSLKGGAYRVAPDMVTVCTLLPPSERHRFEAVGDGC
                     FVSLHADSCREVLSAARRRRVDAIVLSVHRCGGADLPAVARLVREFPSIPAVALVSRH
                     DHHATEILLRLGASGVRAAVDCTDVGGWRRLRELVGQPASPVAARILQSLVPSLGDEV
                     PEDARLFFEALARLAPVIRTVRALARHLRIPPSTLMSRFLRAALPSPKTYLAGMRLVH
                     ASHLFRNPGLSVADVAYRLDFSSPQSFSRHLKAMLGVTAGEYRRRFPFEVALARYVDL
                     LVTPYRETFRAFRPMNVRVGDQGSDAAAVFRAG"
     gene            complement(941..2674)
                     /locus_tag="AC16JC_11825"
     CDS             complement(941..2674)
                     /locus_tag="AC16JC_11825"
                     /inference="COORDINATES: protein motif:HMM:NF012245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR5165427.1"
                     /translation="MTGFEAPRSRPSLATRMRGWTDDAGTEQRLKDLETFARSHRRRW
                     GLITVGALLALIGSFAGTVPTPAGTIVLGAGAFAALNVLIGVIHERGWYRWWLIYAFA
                     LLDVVWVAVLVVWFGHGGLVAAFFIAVLPYAFEQARGVGDFLVLTASLAYLTACYLHG
                     RLYPHTPGLDTGAYLETVTFILVAVGLTRIPATLIARIRLARNVMGEAEQGFLAVRAA
                     AAESDELGFLEQSFNRMLDEIGTTISTVQREADEVAAFAEQLAASAEQIQATSEAVTH
                     TAQDLAGDLARQRGLAEGARGESAKASDQAGALRTRAEMMQVDARRLAATAERGRERV
                     ARAGQTLRTVGEEVRATAATVAGLSGMSDRIGGFAQAIARIARQTHLLALNAAIEAAR
                     AEEHGEGFAVVADEVRTLAAEAARSAREVAELVTELRVGIDAAARAMQTGETQVRDIG
                     AIAAEADGALLELHQGVQLVGDLVNATAEVSRSQAARLAELAQSLQQVAAISSSSSDR
                     ADGAAVSTRSQITSMSDLTATSQQLAQLAERLRASIARFSVMRREQVTAEHRATTSEH
                     HVSRTVTVPGA"
     gene            complement(2715..3713)
                     /locus_tag="AC16JC_11830"
     CDS             complement(2715..3713)
                     /locus_tag="AC16JC_11830"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5165428.1"
                     /translation="MGSFLADESALLSELSALARQGRYREVVDRLRSLAPGVAEARTP
                     LALLAAESHGRLGEHDAADGWAVRALELATRSLDARSEQRATHLRGAIAWQRGAVDEA
                     EGHFQRALELARSLGDDEAQARAFNNLGILQHLRVSPEAALASYQPALAAYQQAGNAR
                     GMAETHHNMAISWRALGNAPRARDADDEAVRLALRAADPTLLGLTLAGRAEAHLMMGD
                     HALAAAELTQAAAAYAHVRFTAGIPEVKRVQAAVARARADLPRAARLLQDARELSRVG
                     VPLHTQAEIERDLGDTLDALGDAAGARAARERARALFHQLGARRAEQALTALLPPG"
     gene            complement(3730..4308)
                     /locus_tag="AC16JC_11835"
     CDS             complement(3730..4308)
                     /locus_tag="AC16JC_11835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165429.1"
                     /translation="MPALIALACSDSVGPANQRPTGLLHILTQDSTAPALVSDTARFY
                     AKVGEDRRVELYYRGLTSDTGEAFLQFEVHAISLLKRPDGSSFQPGDSILITVAVGDP
                     SKFDFTFSPSGLQFNPADPARLKIEYNHSDHDFNNDGVEDAGDTQAESQLAIWQREPP
                     DTLWSRLGTLKHVELDEIEAAILSFTQFAVAW"
     gene            complement(4458..>5244)
                     /locus_tag="AC16JC_11840"
     CDS             complement(4458..>5244)
                     /locus_tag="AC16JC_11840"
                     /inference="COORDINATES: protein motif:HMM:NF012385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR5165430.1"
                     /translation="APFVELNCAALPSALLESELFGHERGAFTDAKAAKPGLFEVATG
                     GTLFLDEIDSLALELQSKLLRALEQKVVRRLGATTARSLDVRIVAATNTRLDRAVAEG
                     RFREDLYYRLNVVTLELPPLRARGDDVLRLVEHFTAKFSKQYDIPEPALTADARRALL
                     AHAWPCNVRELRNIVERTLLLSSPGRFELEPLQAAAPQPPVAGGLPFPAPLAEVQRAA
                     AHAMLELTGGNRSEAARRLGVSRSRLLRLLRGAGDEEPEGEAP"
BASE COUNT          745 a         1829 c         1875 g          795 t
ORIGIN      
        1 gacccagcgc tcgagggacc ttgaacggtc gttgaagggc ggtgcgtatc gtgtcgcccc
       61 ggacatggtc accgtctgca cgttgcttcc gccgtccgag cgtcaccgct tcgaggccgt
      121 cggtgacggg tgcttcgtgt cgctccacgc cgactcctgc cgcgaggtgc tcagcgcggc
      181 gcggcgacgt cgcgtggatg cgatcgtgtt gtcggtccat cgctgcggcg gggcggatct
      241 cccggcggta gcgcggctgg tgcgggagtt cccgtcgatt cccgctgtgg ccctcgtctc
      301 gcgccacgac catcacgcga ccgagatcct gctgcggctc ggcgccagcg gcgtacgcgc
      361 cgcggtggac tgcaccgatg tcggtgggtg gcggcggctg cgggagctgg tgggccagcc
      421 ggcgtcgccg gtggccgcgc gcatcctgca gtccctggtc ccgtccctgg gggacgaggt
      481 gccggaggac gcgcggctgt tcttcgaggc gctggcccgg ctggcccccg tcatccggac
      541 ggtacgggcc ctggcgcgcc acctgcgcat cccgccgagc acgctgatgt cccgcttcct
      601 gcgggcggcg ctgccctctc ccaagaccta cctcgcaggg atgcgactcg tgcacgcgtc
      661 gcatttgttc cgcaatcccg ggttgtccgt ggccgacgtc gcctatcggc tcgatttctc
      721 gtcgccgcag agcttcagcc gtcacctcaa ggccatgctg ggcgtgaccg ccggcgaata
      781 ccgccggcgg tttcccttcg aggtggcgct cgcccgttac gtggatctgc tcgtcacccc
      841 gtaccgggag acgtttcgcg cgttccgccc gatgaacgtt cgtgtcgggg accaaggatc
      901 ggacgccgcg gccgtcttcc gggcggggtg atcgcgagcg tcaggcgccg ggaaccgtga
      961 ccgtgcggga cacgtggtgc tccgacgtgg tggcccgatg ctcggccgtg acctgctcgc
     1021 ggcgcatgac cgagaaccgg gcgatgctgg cgcgcagccg ctccgccagc tgggcgagct
     1081 gctgcgaggt ggcggtgagg tcgctcatgg aggtgatctg ggagcgggtg gacacggcgg
     1141 ccccgtcggc gcggtccgag gagctgctcg aaatcgccgc aacctgctgc agcgactggg
     1201 cgagctcggc cagacgagcg gcctggctgc gcgagacttc ggcggtggca ttgacgagat
     1261 cgccgacgag ctgcacgccc tggtgcagct cgagcagggc gccgtcggcc tcggcggcga
     1321 tcgctccgat gtcccgcacc tgtgtttcgc cggtctgcat ggcgcgggca gccgcgtcga
     1381 tccccacccg cagctcggtg acgagctccg cgacttcccg ggccgagcgc gccgcttcgg
     1441 cggcaagcgt ccgcacctcg tcggcgacca cggcgaagcc ttcgccgtgc tcctcggcgc
     1501 gggccgcctc gatggccgca ttcagcgcga gcagatgcgt ctgccgggcg atccgcgcga
     1561 ttgcctgcgc gaatccgccg atccggtccg acatccccga gagcccggcg acggtggcgg
     1621 cggtggcgcg aacctcctcg ccgaccgtac gcagcgtctg gccggcacgg gccacgcgtt
     1681 cccggccccg ttcggccgtg gcggcgaggc ggcgggcgtc cacctgcatc atctccgcgc
     1741 gggtacgcag cgcgcccgcc tgatccgacg ccttggcgct ctccccccgg gcgccttcgg
     1801 cgagcccccg ctgacgggcg aggtcaccgg cgagatcctg cgccgtgtgc gtcaccgcct
     1861 cggaggtcgc ctggatctgc tcggcggagg cggccaattg ttccgcaaag gcggcgacct
     1921 cgtccgcctc gcgctgcacg gtcgagatgg tggtgccgat ctcgtcgagc atgcggttga
     1981 agctctgctc gagaaagccc agctcgtcgg actcggcggc ggccgcgcgc acggccagaa
     2041 agccctgctc cgcttccccc atcacgttgc gggcgagccg gatccgggcg atcaacgtcg
     2101 ctggaatgcg cgtcaaccct accgccacga ggatgaaggt gacggtctca aggtaggcgc
     2161 cggtatcgag gccgggcgtg tggggataga ggcgaccatg taggtaacag gcggtgaggt
     2221 aggcgagcga ggccgtgagc accagaaaat ccccgactcc gcgggcctgc tcgaacgcat
     2281 agggcagcac agcgatgaag aacgccgcca ccaatccgcc gtggccgaac cagacgacca
     2341 gcacggcgac ccacacgacg tcgagcagcg cgaaggcgta gatgagccac cagcggtacc
     2401 agccgcgctc gtgaatcacg ccgatcaaca cgttgagcgc cgcgaaggct ccggctccca
     2461 acacgatcgt ccctgcggga gtcggcacgg tccccgcgaa gctcccgatg agcgcgagca
     2521 gcgcgccgac cgtgatcagc ccccagcgac gccggtggct gcgggcgaat gtctcgagat
     2581 ccttgagccg ctgctcggtg ccggcgtcgt cggtccagcc acgcatgcgc gtggcgaggg
     2641 agggacgtga gcggggagct tcgaaccctg tcacgacgga atgctactgc gaaaccgtcc
     2701 gttgttccaa tttctcagcc cggagggagg agcgcggtga gggcctgctc ggcccggcgg
     2761 gcgccgagct ggtggaacag cgcgcgggcg cgctcgcggg cggcgcgcgc cccggcggcg
     2821 tcccccaggg cgtcgagcgt atcgcccaga tcgcgctcga tctcggcttg cgtgtggagc
     2881 gggacgccga cgcgggacag ctcgcgcgcg tcctggagca ggcgcgccgc cctcggcagg
     2941 tctgcgcggg ctcgggccac cgcggcctgg acacgcttga cttcggggat tcccgcggtg
     3001 aagcggacgt gcgcataggc ggctgcggcc tgggtgagct ccgcggccgc caaggcgtgg
     3061 tcgcccatca tcaggtgcgc ttccgcccgg ccggcgagcg tcaggccgag caacgtcgga
     3121 tccgcggccc gaagggcgag ccggaccgct tcatcgtcgg cgtcgcgggc gcgcggagcg
     3181 ttgccgagag cccgccagct gatcgccatg ttgtggtgcg tctcggccat gccgcgggcg
     3241 tttccggcct gctggtaggc ggcaagggcc ggctggtaac tggcgagcgc ggcctccggg
     3301 gagacgcgga ggtgctgcag gatgccgagg ttgttgaagg cgcgggcctg ggcttcgtcg
     3361 tcgcccagcg agcgcgccaa ctccagcgcg cgctggaagt gcccttccgc ttcatcgacc
     3421 gccccccgct gccaggcgat cgccccgcgc aggtgggtgg cgcgctgttc ggagcgggcg
     3481 tcgaggctcc gcgtcgcgag ctcgagcgcc cgcacggccc acccgtccgc cgcgtcgtgc
     3541 tcgcccagcc gaccgtggga ttcggcggcg agcagggcga gcggtgtgcg tgcctccgcg
     3601 acgccgggcg cgagcgaccg gagccggtcg actacctcgc ggtatcgacc ctggcgggcg
     3661 agcgccgaca gctccgagag gagcgcggat tcgtcggcga ggaaagagcc catcgtgacg
     3721 gcgagcctcc taccacgcca ccgcaaactg ggtgaacgac aggatggcag cctcgatctc
     3781 gtcgagctcc acgtgcttca gcgtaccgag cctcgaccac agtgtgtcgg gcggctcccg
     3841 ctgccagatg gcgagctggc tctccgcctg cgtgtccccg gcatcctcca ccccatcatt
     3901 gttgaaatcg tgatcggagt ggttgtattc gatcttgagc cgcgcgggat cggctggatt
     3961 gaactgcaga cccgagggcg agaacgtgaa gtcgaatttc gacggatcgc ccaccgccac
     4021 ggtgatgagg atcgagtctc ccggctggaa ggacgacccg tcgggacgct tcaacaggct
     4081 gattgcgtgg acctcgaact gcaggaacgc ctcgccggta tcgctcgtca gaccccgata
     4141 gtacaactcc acacgacggt cttccccgac cttggcatag aacctcgccg tatcggacac
     4201 cagggccggc gccgtcgaat cctgcgtcag gatgtggagc agccccgtcg gccgctggtt
     4261 tgccggaccg acgctgtccg agcaggccag cgcgatcaag gcaggcacca caagagtcct
     4321 catccgcatg tacccagtcc tccgagacga atgtgggcgc tcactaccgc aagcgtcgcg
     4381 ccacgataag taattgttca tcaatgggat acgtccgatc gtcctgctgc ggggcgagcc
     4441 agctgtctca attcgcatca cggagcctct ccttccggct cctcgtcgcc cgcgccccgc
     4501 agcaggcgca gcaggcggga gcgggacacc cccaatcgcc gggccgcctc ggagcggttg
     4561 ccgccggtga gctcgagcat cgcgtgcgcc gccgcccgtt ggacctccgc cagcggcgcg
     4621 gggaagggca aacctcccgc gaccggcggc tgcggcgccg cggcctgcag cggctccagc
     4681 tcgaatcgcc cgggcgacga caggagcagc gtgcgctcga cgatgttgcg gagctcgcgc
     4741 acgttgcagg gccaggcgtg cgccaacaat gcgcgacgcg cgtcggcggt cagtgccggc
     4801 tcggggatgt cgtactgctt ggagaacttc gcggtgaagt gctcgaccag ccggagaacg
     4861 tcgtcgccgc gcgcccgcag gggaggcaac tcgagcgtca ccacgttcag gcgatagtac
     4921 aggtcctcgc ggaagcggcc ctcggcgacc gcccgatcga ggcgcgtgtt cgtagcggcg
     4981 acgatgcgga cgtccagcga cctcgcagtc gtcgccccga ggcggcgaac gaccttctgt
     5041 tccagcgcgc gcagcagctt gctctgcagc tccagggcga ggctgtcgat ctcgtcgaga
     5101 aacaatgtcc cgcccgtcgc gacctcgaac agcccgggct tggcggcctt ggcgtcggtg
     5161 aacgcccctc gttcgtgtcc gaacaactcg ctctcgagca aggcgctggg cagcgcggcg
     5221 cagttgagct cgacgaacgg cgcc
//
LOCUS       DBNGZW010000505         9506 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_526588, whole genome shotgun sequence.
ACCESSION   DBNGZW010000505 DBNGZW010000000
VERSION     DBNGZW010000505.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9506)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_526588"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="AC16JC_11845"
     CDS             <1..467
                     /locus_tag="AC16JC_11845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5165431.1"
                     /translation="YVARVALGADMNQTVRAFAEAESYPGPSLIIAYSPCIAHGYDLS
                     HSLEQQQLSVKSGAWPLYRFDPRRTREHLPPLQLASKDPTVPLATYQRIETRFHAAER
                     LDPARYERLMAQAEREVADRWAVYRYLAGLAVPESPPAEPPATAPEPATVGG"
     gene            516..1316
                     /locus_tag="AC16JC_11850"
     CDS             516..1316
                     /locus_tag="AC16JC_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165432.1"
                     /translation="MIPARAPWFACVLLAAVAVLTGCREKKEQKAPGGAVVFQISAHH
                     PTAADTAASLAINDTMLVHGRDTLFLRVARIVLGGLALAPAVSNECEEEEGEDNPPCV
                     EFDDEPVVIELPLGGGATNRSTKPAPATDYNLFQAMIHRPDSVSAHDVQLLLHHPELW
                     ARSIRVEGTLSRGGRRRAFTFNSDFNETEEITLEPALTVKPGDTLHVTLRVDVASWFR
                     TEDGRSFVDPATAGLAGRNESLVRGNIRTSLKVFRDSNADGLEDGTSP"
     gene            1508..2299
                     /locus_tag="AC16JC_11855"
     CDS             1508..2299
                     /locus_tag="AC16JC_11855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165433.1"
                     /translation="MNGSRLEKSRWFAAAIALVGAAACSDSSAGGTVSLSLSARAAAG
                     AAAAPAVTGNLAAPAVLAAGDTTIITLGSDTIVLRSVDVVLRKIELKRVEASSCDSVP
                     DNGDCEEFESGPVLASFPLGTTNTVAAVTVAAPAGQYDQLEFEIHRTDTTSSQEAAFI
                     AANPDFKNISIRVTGTFNHTAFVYTSDLDASQEMALAAPLDVTAGTSANLTIRMDVST
                     WFVSNGALVDPASANKGGQNEGIVKENIKQSIEAFEDDNHDGLEG"
     gene            2501..3205
                     /locus_tag="AC16JC_11860"
     CDS             2501..3205
                     /locus_tag="AC16JC_11860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165434.1"
                     /translation="MVHASPASRARWLGPLVLILAACRDMGPSRSLSLSVTAKSVATA
                     SLPAAPGLNADIVVGSGTSAITISKVQVVMSEIELSTGGTCSSTGEHDNCDELEVGPV
                     PIDLPVDGTTQVFLDKVVPPGTYTALEAKVDSVMVTFTDANNQTHDFKLALGEDAEIE
                     AAFQAPVTVDATTSNLTINVDVASWFKDATGAVLDPTNPANASLIHQNILRSFHAFED
                     DNHDGEDDGLEGGGDL"
     gene            3265..4125
                     /gene="ilvE"
                     /locus_tag="AC16JC_11865"
     CDS             3265..4125
                     /gene="ilvE"
                     /locus_tag="AC16JC_11865"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009202284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HIR5165435.1"
                     /translation="MTTIWIDGKYFDRDTAAISVFDHGLLYGDGVFEGIRAYAGRIFR
                     LDAHLDRLYASAKGICLDIPLARPQMTTMVSQAYAQSAMRDAYLRLVVTRGVGDLGLD
                     PRKCGKPTVICIVDTIRLWPQDRYDNGLTCLTAATPISHRENLSPRIKSLNYLAHILA
                     KIEGIAANVDEVVMLDASGYVTEASGMNLFAVANHTLRTPPPWSGILRGVTRDAVMEL
                     ARDAGYAVEETPLNRYDLYTADEVFLTGTAAEVVGITRLDGRTIGAGTAGPITKDMAI
                     RFHALATSGD"
     gene            4392..4640
                     /locus_tag="AC16JC_11870"
     CDS             4392..4640
                     /locus_tag="AC16JC_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165436.1"
                     /translation="MSHPELHDLRRVVAAVERLRGAAVSGVTVRSDLRHLKVDLVGGL
                     ILLIAAERDAQGRPRLEVDVIEAPQDPSAQNQIEVHFD"
     gene            4649..5068
                     /locus_tag="AC16JC_11875"
     CDS             4649..5068
                     /locus_tag="AC16JC_11875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165437.1"
                     /translation="MSGRTATAERLRFQVFGFDRLSDGRCRARVQLAWTDGREFVGVA
                     EGMTSQAGELRCSAEAAVQALTEAVRPGVELELLGVKAVRAFDATVVIVSLAVRSEDS
                     AWRMVGSYLTETDPPRGAALAVLNATNRVLGNYVTTR"
     gene            5065..6585
                     /locus_tag="AC16JC_11880"
     CDS             5065..6585
                     /locus_tag="AC16JC_11880"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR5165438.1"
                     /translation="MTTHTSKTSSLFLLAGLAACAGHQQSVRMAPLPAPPPAAQPVVA
                     AAPAPAAGAGDTVAPAATVRSEDVEREAVRLFGPEGREIVGAKAVADDTSVPTFDIDV
                     TSYAANRRVLEYLEFFQVDARDRMAIWLSRLSRYEGMIRDRLRARGLPEDLVYLTLIE
                     SGLSNSAVSRARAVGMWQFVAGTGRLYGLTIDPWVDERRDPFRATDAAVDYLSDLVQK
                     LGSVYLAAAAYNAGEGRIQRGVRRLPGEADSLTDETFFQLASTRILRRETRDYVPKLI
                     AAALVAKQPSRYGFTDISQLPPLTFDEIRVPDATGLDVLARLADTTTAALLELNPQFV
                     RGVTPPGREVTVRIPRGRGTLVAERFDTLPVTDRTTFVDHYVTRGQTLSDIAKQYRVS
                     VSMIQGANPSLKPRTLRVGQRLVIPMSGRVVPRSAWNRPSERTAAPRTTRAPGATISA
                     SHRVRSGETASQIARRFGVTLRSLLDYNGLTLTSLLKPGQLLRIPAPNGAGRRQAE"
     gene            complement(6725..7381)
                     /locus_tag="AC16JC_11885"
     CDS             complement(6725..7381)
                     /locus_tag="AC16JC_11885"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR5165439.1"
                     /translation="MSASRRWQMQGGVIAVSVLAIASFGWARHATADEQPPAAATLSE
                     MRTLSQQLESLRGELTVARLQLDRADAVMEFSGRYHIPADLTAAIYDIALAEGIEPGL
                     AFRLVSVESRFDARAHSPAGAVGLTQILPSTARLFEPSLTVDQIYERETNLRLGFRYL
                     HDLLVRYDDNLEHALLAYNRGPSKVQELVTAGVDPSNGYAQSVMRGYRRQARQTAGAE
                     "
     gene            complement(7402..7836)
                     /gene="moaC"
                     /locus_tag="AC16JC_11890"
     CDS             complement(7402..7836)
                     /gene="moaC"
                     /locus_tag="AC16JC_11890"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007067365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061799 - cyclic pyranopterin
                     monophosphate synthase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HIR5165440.1"
                     /translation="MVDVGGKPVTARSATAEGAIRMSREAFRLVAEGTVPKGDVLTVS
                     RIAGTLAAKRTAELIPLCHPLGLDHVEVQATLEEALPGVRVRATVRTVGRTGVEMEAL
                     TAVSVALLTVYDMVKSADREMELLSVRLLEKTGGSRGDWRRG"
     gene            complement(7866..8900)
                     /locus_tag="AC16JC_11895"
     CDS             complement(7866..8900)
                     /locus_tag="AC16JC_11895"
                     /inference="COORDINATES: protein motif:HMM:NF024575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HIR5165441.1"
                     /translation="MSLRPLYGHEALRRRLGTAASGGRLPQALLLEGPEGVGKQRLAL
                     WLAQVLLCERGGAEPCDACRSCKMANSLTHPDLHWLVPLEPSKKTADSDKQVEVVAGA
                     LADEIAARRDRPLYGPLPGMAVHSVAVVRHMLRHVALTPAMASRKVFIVGDAERLIPQ
                     RANPEAANALLKVLEEPPVDTVLILTASEPDALLPTILSRVVRVRVPRVPDSVVTAFM
                     HKELPESKLAASEADGCIGRVLARGQGGAAPEREVAGAFQASLRSAVDRYAFALRQAP
                     FQARGGFTDMLDGLVDRLRAEARRGGDTSRLAGAIALVLRAREQAQGNVNPQLLTAVL
                     SGDLDGQWPT"
     gene            complement(9069..>9506)
                     /locus_tag="AC16JC_11900"
     CDS             complement(9069..>9506)
                     /locus_tag="AC16JC_11900"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5165442.1"
                     /translation="LPLSTEQLPGGEVATPPSTPAMGSETVLLVEDEPAVRQFARRAL
                     EASGYCVLAAGDGSEALSLAEQHAGIIHVLLTDVVMPGMGGPELARRLSLRRPGLRVL
                     YCSGYTDDAAIREGVREAGTAFLQKPFAPEDLIHKIREVLATP"
BASE COUNT         1549 a         3240 c         3167 g         1550 t
ORIGIN      
        1 tctacgtcgc ccgggtggcg ctcggcgcgg atatgaacca gacggtccgc gccttcgccg
       61 aggcggagag ctaccccggg ccgtcgctca tcatcgccta ctcgccctgc atcgcgcacg
      121 gctacgacct ctcgcacagc ctggagcagc agcagttgtc ggtgaagtcg ggcgcgtggc
      181 cgctgtaccg cttcgatccg cgccgcacgc gggagcacct gccgccgctg cagctcgctt
      241 cgaaggaccc gaccgtcccg ctcgcgacct accagcggat cgagacgcgc tttcacgcgg
      301 cggagcgact cgacccggcg cgctacgagc ggctgatggc gcaggccgag cgcgaagtcg
      361 cggaccgctg ggccgtgtac cgctatctcg cggggctggc ggtcccggag tcgccgcccg
      421 cggaacctcc cgctaccgct cctgagccgg cgaccgtggg agggtagggg cgcgcgagtt
      481 gcacgagtca acacgagccg cataggttcc gtgtcatgat ccccgctcgc gcgccgtggt
      541 tcgcctgcgt cctcctggca gcagtcgccg ttctcaccgg gtgtcgggag aagaaggagc
      601 agaaggcccc gggcggggcc gtcgtcttcc agatctccgc ccaccatccg accgccgccg
      661 ataccgcggc ctcgctcgcc atcaacgaca ccatgctcgt gcatggccgg gatacgctgt
      721 tcctgcgagt cgctcgcatc gtgctcggcg gactggcgct ggcgcccgcc gtgtcgaacg
      781 agtgcgagga agaagaaggg gaggacaacc ctccctgcgt cgagttcgac gacgagccgg
      841 tggtgatcga gctgcccttg gggggcgggg cgaccaaccg ctcgacgaag ccggcgccgg
      901 cgaccgacta caacctgttc caggcgatga tccaccgccc cgattccgtc tccgcccacg
      961 acgtccaatt gctcctgcac caccccgagc tctgggccag gtcgatccgc gtggagggaa
     1021 cgctcagccg cggcggcaga cgtcgcgcct tcaccttcaa ctcggacttc aacgagaccg
     1081 aggagatcac gctcgaaccg gcgctcacgg tgaagcccgg cgacacgctc cacgtcaccc
     1141 tgcgcgtgga tgtggcctcc tggttccgca ctgaggatgg ccggtccttc gtcgatcccg
     1201 cgacggcggg gctcgcgggg cggaacgaga gcctggtgcg cggcaacatc cgcacgtccc
     1261 tcaaagtgtt tcgcgattcg aacgccgacg ggctcgagga cggcacgtct ccgtagccct
     1321 gcctcgaagc gcttcgctgc atcccattcc tgcgccgttt cgatacagcc ccagaggtgt
     1381 gtgaccccgc gcacgtccgc taactccttg gtggacaagt aaatggccgc caggccatag
     1441 ggcacgcgtc gtgcgagacc ggcgggtcgt tcagctcgat gcacacacct gaaacccgga
     1501 gcgcacgatg aacggcagca ggctggagaa gtcccgttgg ttcgccgcgg cgattgccct
     1561 ggtcggggcg gccgcgtgtt cggatagcag cgccggcggt accgtatccc tctccctttc
     1621 cgcccgggcg gcagcgggcg cggcggcggc gccggcggtg accggcaacc tcgcagcgcc
     1681 ggccgtgctc gccgccggtg acaccaccat catcacgctg ggcagcgaca cgatcgtgct
     1741 gcggagcgtc gacgtggtgc tgcgcaagat cgagctgaag cgggtagagg cgtcttcctg
     1801 cgacagcgtt cccgacaacg gcgactgcga ggagttcgaa tcgggaccgg ttctggcgtc
     1861 cttccccctc ggaaccacga atacggtcgc ggccgtgacc gtggcggcgc cggccgggca
     1921 gtacgaccag ctggagttcg agattcaccg caccgacacg acctcgagtc aggaagcggc
     1981 attcatcgca gccaacccgg acttcaagaa catctcgatc cgggtgacgg gcaccttcaa
     2041 ccacaccgca ttcgtctaca cctcggatct cgatgcctcg caggagatgg ctctggcggc
     2101 cccgctcgac gtgacggcgg gcacgagcgc caacctgacg atccgcatgg acgtctccac
     2161 ctggttcgtg agcaacggtg cgctggtcga tcccgcttcg gcgaacaagg gcgggcagaa
     2221 cgaggggatc gtgaaggaga acatcaagca gtcgatcgag gcgttcgagg acgacaacca
     2281 cgacggcctc gaagggtaac cgagcggctc gcgctgtctc acgacatacc agcttgacct
     2341 gtttcgatgc acgttgccct atgaccggcg ctgctggtca tggggcaacg tgttgtatgc
     2401 caatggactc caccgcatga cgcgccgggg cagtacgggc atatcgcctg caaaccacgc
     2461 cgaccggtgt ctccgctctc attccttcct cgaggtgcct atggtgcacg catcccccgc
     2521 atcccgcgcg cggtggctcg gtccgctggt gttgatcctg gccgcctgcc gtgacatggg
     2581 accttccaga tcgctcagcc tctcggtcac cgccaagtcg gtggcaactg cgtcgctgcc
     2641 ggctgcgccg ggcctcaatg ccgatattgt ggtcggcagc ggcacgagcg cgatcaccat
     2701 cagcaaagtg caggtcgtga tgtccgaaat cgagctgtcg accggcggca cgtgctccag
     2761 caccggcgaa cacgataatt gtgacgagct cgaagtgggg ccggtcccca tcgacctgcc
     2821 tgtcgacggc accacgcagg tgtttctcga caaggtcgtg cccccgggca cgtacaccgc
     2881 cctcgaggcg aaggtcgaca gcgtcatggt gaccttcacc gacgcgaaca atcagaccca
     2941 cgatttcaag ctcgcgttgg gggaagacgc ggagatcgag gcggccttcc aggcgcccgt
     3001 cactgtggat gccaccacgt ccaacctcac gatcaacgtg gacgtggcga gctggttcaa
     3061 ggatgcgact ggtgccgtgc tcgatccgac gaacccggcc aacgccagtc tcatccacca
     3121 gaacatcctg cggtcgttcc atgcgttcga agacgacaac cacgacggcg aagacgacgg
     3181 actggaaggg ggaggagacc tctagtcggg cgccgctgac agggttgtca tcgtccgccg
     3241 ggggagcgag attactcccc cggcatgacg accatctgga tcgacgggaa gtacttcgac
     3301 cgcgacacgg ccgcgatcag cgtcttcgac cacggcttgc tgtacggcga cggcgtgttc
     3361 gagggtatcc gggcctatgc ggggcgcatc ttccggctcg acgcgcacct cgaccgattg
     3421 tacgcctcgg ccaagggcat ctgtctggac attccgttgg cccgccccca gatgaccacg
     3481 atggtgtccc aggcgtacgc ccagagcgcc atgcgcgacg cctacctccg gctggtggtc
     3541 actcgtggtg tgggcgacct ggggctcgat ccccgcaagt gtggcaagcc cacggtcatc
     3601 tgtatcgtgg acaccatcag actctggccc caggaccggt acgataacgg cctgacgtgc
     3661 ctgaccgccg ccacacccat cagtcaccgc gagaaccttt ctccccgcat caaatccctg
     3721 aactacctgg ctcacatcct ggccaaaatc gaaggcatcg ccgccaacgt ggatgaggtg
     3781 gtgatgctgg acgcctcggg ctacgtcacc gaggccagcg gcatgaacct gttcgccgtc
     3841 gccaaccaca cgctgcgcac gccgccccct tggagtggca ttctccgggg ggtgacgcgg
     3901 gacgcggtga tggagttggc gcgcgatgcg ggttacgcag tggaagagac ccccctgaac
     3961 cgctatgacc tctacacggc ggatgaggtg ttcctcaccg gcaccgccgc tgaggtcgtt
     4021 gggattacac gcctggatgg tcggaccatc ggcgccggta cggccggccc gatcacgaag
     4081 gacatggcca tccgattcca cgccctggcg acgtcaggcg actgaagtat tgacctgact
     4141 tgcagttagg gtcatattga gcggccaatt gaggggtcat tgacggcctg gtgtcctcac
     4201 ttaacgcgaa gaaaacctgt tgttgaggcc ctggcggcac gtcgtgtcgt ttcgcgcgaa
     4261 ctcgggcagg atgcaggaat ctgcgctgtg gacgagttta ccccggaggt ctcgtcccaa
     4321 tgggcactat ttctgcaggc ggaacgcatg gccggcgtcg tgtgggggcg gctgctgacc
     4381 gagcgctcgg cgtgagccat ccggagctcc acgatctgcg tcgcgttgtg gcggcggtgg
     4441 agcggctgcg cggggcagcg gtgagtggtg tgacggtccg gtccgacttg cgtcacctga
     4501 aggtggacct cgtgggtggt ctgatcctgc tcattgcagc ggagcgggat gcccaggggc
     4561 ggccacggct cgaggtggac gtcatcgagg cgccccagga tccgtctgcg cagaaccaga
     4621 tcgaggtgca cttcgattga gtgaccgggt gagcggcaga acggcgaccg ccgagcggct
     4681 gcgctttcag gtgttcgggt tcgaccgcct ttccgacggc cgttgccggg cccgggtgca
     4741 gttggcgtgg acggacggtc gggagttcgt gggcgtggcc gaaggcatga cgtcccaggc
     4801 gggggagctg cgctgctccg cggaggcggc ggtgcaggcg ttgacggaag cggttcggcc
     4861 cggcgtcgag ctggagcttc tgggagtgaa agccgtccgg gccttcgacg cgacggtcgt
     4921 gatcgtgagt ctggcggtgc gctcggagga cagcgcgtgg cgcatggtcg gctcgtactt
     4981 gacggaaacc gatccgccgc gcggggcagc cctggcggtc ctgaacgcga ccaaccgcgt
     5041 tctggggaac tatgtgacga cacgatgacc acacacacgt cgaagaccag ctccctgttc
     5101 ctgctcgcag gccttgcggc ctgcgccggc caccagcaat cggtgcggat ggcgccgctg
     5161 ccggccccgc ctcccgcggc gcagcccgtc gtggccgcgg cgcccgcccc ggcagccggg
     5221 gcgggagaca ccgtggctcc ggcggcgacc gtgcgcagcg aggatgtgga gcgcgaggcg
     5281 gttcgcctct ttgggccgga agggcgggag atcgtggggg cgaaggccgt cgcggacgac
     5341 acgagcgtcc cgaccttcga catcgacgtg acgagctacg ccgcgaaccg ccgggtgctc
     5401 gaatacctgg aattcttcca ggtcgacgcc cgggatcgca tggccatctg gctgtcccgg
     5461 ttgtcccgtt acgaggggat gatccgcgat cgcctgcggg cgagggggct gcccgaggac
     5521 ctggtgtatc tgaccctgat cgagagcggc ctgtccaatt cggccgtcag ccgggcccgc
     5581 gcggtcggca tgtggcagtt cgtcgccgga acggggcggc tgtacggcct cacgatcgat
     5641 ccgtgggttg acgaacggcg cgaccccttc agggcgaccg acgcggcggt ggactacctg
     5701 tcggacctcg tccagaagct cgggagcgtc tacctggcgg cggccgccta caacgccggc
     5761 gagggccgga tccagcgcgg tgtgcggcgg ttaccgggcg aggcggatag cctcaccgat
     5821 gagaccttct tccagctcgc cagcacgcgc atcctgcggc gcgagacccg ggactacgtc
     5881 cccaagctga tcgcggccgc cctggtcgcc aagcagccct cgcgctacgg gttcaccgac
     5941 atctcgcagc ttcctcccct gaccttcgac gagatccggg tgccggatgc cacggggctc
     6001 gacgtcctgg cgcggctcgc cgatacgacc acggcggcat tgttggagct caatccgcag
     6061 ttcgtgcgcg gcgtgacccc cccgggacgc gaggtgaccg tgcggatacc gcgcggtcgg
     6121 gggacgctgg tggcggagcg cttcgacacg ctgcccgtga cggaccggac gaccttcgtg
     6181 gaccattacg tgacccgggg gcagaccctc tccgatatcg cgaagcagta ccgcgtctcg
     6241 gtctcgatga tccagggtgc caacccgtcg ctcaagcccc gcacgctgcg tgtgggccag
     6301 cggttggtca ttccgatgag cgggcgcgtg gtcccgcggt cggcgtggaa tcggccgtcg
     6361 gagcggaccg ccgccccccg gacgacgagg gcgccgggcg ccacgatctc ggcctcccac
     6421 cgcgtccgct cgggcgagac ggcgagccag atcgcccggc ggttcggggt gacgctccgg
     6481 tcgctcctgg actacaacgg tctcacgctg acgtcgcttc tcaagcctgg tcagctgctg
     6541 cggatcccgg cgccgaacgg ggcggggaga cgtcaggcgg agtagctcgc tccgcccggt
     6601 cggctgtcga gcgtcgggcg cggggacagc cgcgaacgct tctcacgatg acgtcgattg
     6661 tccagcctgg tctgctgctg cggatcccgc cgccgaacgg ggcggggagg tcaggcggag
     6721 tgactcactc cgcgcccgcc gtctggcggg cctggcgccg gtagccgcgc atcaccgact
     6781 gcgcatagcc gttcgaggga tccacgccgg cggtgacgag ctcctggacc tttgacgggc
     6841 cgcggttgta ggcgagcagc gcgtgctcca ggttgtcgtc gtagcggacc agcaggtcgt
     6901 gcaggtagcg gaaccccagc cgcaggttcg tttcccgttc atagatctga tcgaccgtga
     6961 ggctgggctc gaacaacctg gccgtgctgg ggagaatctg ggtcaacccg acggcgccgg
     7021 ccggactgtg ggcccgagcg tcgaaccggg actcgacgct gaccagccgg aaggcgaggc
     7081 cgggctcgat cccctcggcc agcgcgatat cgtagatcgc cgccgtcaga tcggccggga
     7141 tgtggtagcg gcccgagaac tccatgaccg catcggcccg gtcgagctga agccgcgcca
     7201 cggtcaactc gccccggagc gactcgagct gctgcgacag cgtccgcatc tcgcttagcg
     7261 tcgccgccgc gggcggctgc tcgtcggccg tggcgtgacg ggcccagccg aagctggcga
     7321 tggccagcac gcttaccgcg atcaccccac cctgcatctg ccaccttcgt gaagcgctca
     7381 tacccctccc gatcatcgtc ctcagccgcg gcgccaatcg ccccggctgc ctcctgtctt
     7441 ctccagcaac cgcaccgaca ggagctccat ctcccgatcc gcgctcttta ccatgtcgta
     7501 cacggtcaga agagcgactg aaacggcggt gagagcttcc atttccaccc cagtccgccc
     7561 caccgtgcgg accgtggccc gtacccggac cccgggcaac gcctcctcca aggtggcctg
     7621 cacctcgaca tggtcgaggc cgagggggtg acacagcggg atcagctccg ccgtgcgctt
     7681 ggccgccagg gtccccgcga tccggctcac cgtgagcacg tcgcccttcg ggaccgtacc
     7741 ctctgccacg agccggaacg cctctcgtga catcctgatc gctccctcgg cggtggcact
     7801 tcgagccgta accggctttc cgccgacatc caccatccgg gcgctgccgc cctcgtccag
     7861 gtgggtcagg tcggccactg gccgtccaaa tcccccgaca gcaccgcggt cagcaactgc
     7921 gggttcacgt tcccctgcgc ctgctcccgg gcccgcaata ccaaggcgat cgcgccggcc
     7981 agccggcttg tatcgccgcc acggcgggct tctgcccgga gccgatccac caggccgtcc
     8041 agcatgtccg tgaagccacc ccgtgcctgg aacggcgcct gacgcagcgc aaacgcgtaa
     8101 cggtcaacgg cactacgcag gctcgcctgg aacgctccgg ccacctcccg ctccggggca
     8161 gccccgcctt gccctcgggc aagaaccctg ccgatacagc catcggcctc agatgccgcc
     8221 agttttgatt cagggagctc tttgtgcatg aatgctgtca cgacactatc ggggacccgc
     8281 ggcaccctta cccgcacgac gcgtgagagg atcgtgggga ggagggcatc gggttccgag
     8341 gcggtcagga tgagcacggt atccaccggg ggctcttcca gtaccttcag cagggcattg
     8401 gcggcctcgg gattagcgcg ctgagggatg aggcgctccg catccccgac gatgaacacc
     8461 ttgcgactgg ccatagctgg cgtcagcgca acatgtcgta gcatgtgtcg cactaccgca
     8521 acactgtgta cggccatccc gggcagtggc ccgtaaagcg gccgatcacg tcgggccgca
     8581 atctcgtccg ccaaggcacc ggctacgacc tccacctgct tgtccgagtc ggccgtcttc
     8641 ttcgacggct cgagcgggac gagccagtgc aggtcggggt gggtgaggga gtttgccatc
     8701 ttacacgagc ggcaggcgtc gcacggctcc gctccgcctc gctcacacag cagcacctgg
     8761 gcgagccaca gggccaagcg ttgcttcccg acgccttccg ggccctccag cagcaaagcc
     8821 tgaggcaaac ggcccccact cgcggcagtc cccaagcggc gacgcagggc ttcgtgaccg
     8881 tataaagggc gaagagacat gggcgcaatc tagcgggggg gcggtcaact gcaggtcagg
     8941 cttatggcaa ctcttttgtt caatgttact tacggcatca ttccagggct tgtgtggtaa
     9001 taagtcattt atattgaccg agttacgata aaaagtgagt acacactcgc tatgtttcca
     9061 tcatttcctc acggcgtggc cagcacctcc ctgatcttgt gaatcaaatc ctccggggcg
     9121 aagggcttct gcaggaaagc cgtccccgct tctcgaacac cctcgcggat ggcggcgtcg
     9181 tcggtgtagc ccgagcagta caggactctc aggccgggtc ggcgcagcga cagcctgcgg
     9241 gccagctcgg ggccacccat cccgggcatc acgacatcgg tgagcaatac gtggatgatg
     9301 ccggcatgct gttccgccag gctcagtgcc tcggacccgt cgccggccgc cagcacgcag
     9361 tagccgctcg cctcgagcgc ccgacgggca aactgccgca cagcgggctc gtcctcgacc
     9421 aggagcaccg tctccgaacc catggccggt gtgctcgggg gagtcgcgac ctcgccgccg
     9481 ggcagctgct cggtgctgag cggcag
//
LOCUS       DBNGZW010000506         2344 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_593119, whole genome shotgun sequence.
ACCESSION   DBNGZW010000506 DBNGZW010000000
VERSION     DBNGZW010000506.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_593119"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="AC16JC_11905"
     CDS             complement(<1..175)
                     /locus_tag="AC16JC_11905"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR5165443.1"
                     /translation="MTCAELETRLHAYLDAELPEAAAREVAAHVVGCPHCGPWYEEQR
                     TLRAALRDRLPAYR"
     gene            complement(172..702)
                     /locus_tag="AC16JC_11910"
     CDS             complement(172..702)
                     /locus_tag="AC16JC_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5165444.1"
                     /translation="MVDSTSLERFEAVVLPHLDSAYNLARYLTRDLHDAEDVVQEAYL
                     RALRYFGGYRGGDARAWMLAIVRNTFHTWRRRERHQDEAMEFDEVRHSEVIEGAHPGA
                     LLDTALDREALERALERLPTEFREVLVLRELEGMSYKEIGTVVGVPVGTVMSRLARAR
                     ERLRRSLLAGTDAERA"
     gene            842..1828
                     /locus_tag="AC16JC_11915"
     CDS             842..1828
                     /locus_tag="AC16JC_11915"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014368896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5165445.1"
                     /translation="MEQRRLGSQGLAVSALGLGCMGMSDFYTGRDDIESVATIHRALE
                     LGITFLDTSDVYGPFTNERLVGHAIADRRDAVVLATKFGNVRGPDGAWLGVNGRPEYI
                     HAACDASLQRLGVEHVDLYYQHRVDPAVPIEETVGALGDLVRAGKVRFIGLSEAAPAT
                     IRRAHAVHPVAAVQTEYSLWTRDPEDGVLATCRELGIGFVAYSPLGRGFLTGRIRRTT
                     DLPADDVRSRHPRFQEANFGRNLTLVEHVELLARAKRCTPAQLALAWVLAQGTDIVPI
                     PGTKRRPYLEENLGGLDVRFTPEELRTIDRAIPRNSASGLRYPEAAMRGLNI"
     gene            1839..>2344
                     /locus_tag="AC16JC_11920"
     CDS             1839..>2344
                     /locus_tag="AC16JC_11920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR5165446.1"
                     /translation="MKAVGFSAPGGPDVLRLEEVPDLEPGPRQLVMQVEAAGVNYIDL
                     YQRAGQYKTVLPFIPGQEAAGTVAALGPGVSGFAVGDRVVCYKGALAAYAEYVLAPAD
                     RVVRLPEGVSAEQGAAVMLQGMTAHYLACTTYPLKPGDTCLVHAAAGGTGLLLCQIAR
                     KRGARVIGT"
BASE COUNT          349 a          822 c          797 g          376 t
ORIGIN      
        1 cccggtaggc ggggagccgg tcgcgcagtg cggcgcgcag cgtccgctgc tcctcgtacc
       61 acggaccgca gtgggggcat ccgacgacgt gcgccgccac ttcccgggct gccgcttccg
      121 gaagctcggc atccaggtac gcgtgcaggc gcgtctcgag ctcggcgcag gtcatgcgcg
      181 ttccgcgtcc gttccggcga gcagcgaccg gcgcagtcgc tccctggccc gcgccagccg
      241 cgacatcacg gtgccgacgg gcacgccgac cacggtgccg atttccttgt acgacatccc
      301 ttccagctcg cgcagcacca gtacctcccg gaactccgtg ggcagccgtt cgagcgcccg
      361 ctcgagcgct tcccggtcca gcgccgtgtc gagcagagcg ccgggatgcg ctccttcgat
      421 cacctcgctg tgccggacct catcgaactc catcgcctcg tcctggtgcc gctcgcgccg
      481 ccgccacgtg tgaaacgtgt tgcgtacgat cgccagcatc caggcgcgtg cgtccccgcc
      541 gcggtacccg ccgaagtatc tcagggcacg cagatacgcc tcctgcacca cgtcctcggc
      601 gtcgtgcaaa tcgcgcgtca ggtagcgggc caggttgtag gccgaatcca ggtgcggcag
      661 caccacggct tcgaaccgct cgagactggt ggaatccact atccctcggg aagcgagact
      721 ctctccggcc ccggattatt ccccgtggcc ttggccggcc ggtcctacac cggaaccggg
      781 caccgggatg tcaggtttcg gagtacactt ccagaataac ctcgatgcag ggagaggagc
      841 gatggagcag cgacgactgg gatctcaggg acttgccgtg tcggccctcg gactgggctg
      901 catgggcatg tcggatttct atacagggcg ggacgacatc gagtcggtcg ccacgattca
      961 tcgcgccctg gagctcggca tcacctttct cgacacctcc gacgtctatg ggccgttcac
     1021 caacgagcgc ctggtcggtc acgccatcgc ggaccggcgc gacgcggtgg tgctggcgac
     1081 caagttcggc aacgtccgcg gccctgacgg ggcctggctg ggcgtcaacg ggcggcccga
     1141 gtacatccac gccgcctgtg acgcgtcgct ccagcgcctc ggcgtcgagc acgtcgacct
     1201 ctactaccag caccgcgtgg acccggcggt accgattgag gagacggtcg gcgccctggg
     1261 ggacctcgtg cgcgcgggca aggtccggtt catcggcctc tcggaggccg ccccggccac
     1321 gatccggcgc gctcacgccg tccatcccgt agcagcggtg cagacggagt actcgctctg
     1381 gacccgtgat cccgaagacg gtgtgctcgc gacctgcagg gagctgggga tcgggttcgt
     1441 ggcctacagc ccgctggggc gcgggttcct gaccggccga attcgccgca ccaccgatct
     1501 cccggccgat gacgtccgca gccggcaccc gcgatttcag gaggccaatt tcggtcggaa
     1561 cctgacgctc gtggagcatg tcgagctgct cgcgcgcgcg aaacggtgca caccagccca
     1621 gctggcgctc gcctgggtgc tggcccaggg gacggacatc gtcccgatcc ccggcaccaa
     1681 gcggcgcccc tatctcgagg aaaacctggg cgggctggat gtgcgtttca cgccggagga
     1741 gctgcgcacg atcgatcggg ctattcccag gaattccgcc tcgggcctcc ggtatcccga
     1801 agccgccatg cgcggcctca acatctgagg ggccaccgat gaaggccgtc ggcttctccg
     1861 ctccgggcgg tcccgacgtc ctgcgcctcg aagaggtccc ggacctggag cccggaccgc
     1921 gccagctggt gatgcaggtc gaagctgccg gcgtcaatta catcgatctg taccagcgcg
     1981 cggggcaata caagaccgtg ctgccgttca ttccgggcca ggaggccgcg ggtaccgtcg
     2041 cggcgctggg ccccggggtc agcggcttcg cggtgggaga ccgtgtggtt tgctacaagg
     2101 gtgcgctggc tgcgtatgcc gagtacgtgc tggctccggc ggaccgcgtg gtgcgacttc
     2161 ccgaaggtgt gagcgcggag cagggcgccg cggtgatgct ccaggggatg accgcccact
     2221 acctcgcctg cacgacctat cccctcaagc ccggcgacac gtgcctggtg catgcggccg
     2281 cgggggggac cgggttgctg ctgtgccaga tcgccaggaa acgcggggcg cgggtgatcg
     2341 gcac
//
LOCUS       DBNGZW010000507         4236 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1057304, whole genome shotgun sequence.
ACCESSION   DBNGZW010000507 DBNGZW010000000
VERSION     DBNGZW010000507.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4236)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4236)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4236
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1057304"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="AC16JC_11925"
     CDS             <1..717
                     /locus_tag="AC16JC_11925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165447.1"
                     /translation="PLTADQLLAGAEADLHAGAVEANPNRANALALLSHLLARKSETV
                     DAKLTAMQAYEVDPYLSDAEGVLGRLTLASLDLEDAAETNKWCQEGYRRFPQSPQFTE
                     CQIQVHALKGQKPDVPQLWRLLDQDVAQYPPSDRDFRRRRGSLFVAMALANVGLKDSA
                     RAVALRARAGADVDPSRDLIYVEILLRNVLGDRDEAIRLLKLYLVTNPQDKATIASDS
                     TWWWRGLRGDAEFRQLVGLK"
     gene            991..2169
                     /locus_tag="AC16JC_11930"
     CDS             991..2169
                     /locus_tag="AC16JC_11930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165448.1"
                     /translation="MQAVVTATSAPPCDFKTLSQLATHYFGSVEAKVVRGIISRMQDS
                     GAFSLTAKNGGFDVMTHIAANVKAGNTGVADASNMTNGLLACMFNNPADLPPTFPEDF
                     TIATNPALHGAYDVRGGAGDLTGPVLSRPLDAPLSGVAPSGSNTWPGVLSGNPAPARV
                     LIYGQPGLVPNTFDWRAAPFSVNFSPAVIVGVCLDAKANATSLIHEEHVGLLPFADAA
                     FLPTCTGLASQSWGSQFASRLVRWGMSVLGPRPLSATTFLNPGGLAGSTGSLRSEFGP
                     QLVDTVTLTFTVQPSDVKVNSFITPAVVVRATAVTDFGIDSVPNVSITLSAFNNNGTP
                     AILNGTLTQTTGADGKATFADLSETKTGGYVLVATGTVNGRAAILVPATTSARFNVKP
                     "
     gene            2279..3559
                     /gene="aroA"
                     /locus_tag="AC16JC_11935"
     CDS             2279..3559
                     /gene="aroA"
                     /locus_tag="AC16JC_11935"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HIR5165449.1"
                     /translation="MKVSGVVRVPGDKSISHRVLILAAMARGESTLTGLLDGADVKSS
                     ARILRQLGADVSPLREGAVVRVRGRRFRSPQTRLDCGNSGTTARLCIGAIAGHPIRAQ
                     LTGDASLRRRPMRRVIEPLRQMGARFVPVETDTLPLMVRGGRLHGLTYASPVASAQVK
                     SAILLAALAGRVPVTITEPWRSRDHTERLFVHLGLELHETDGAISYRPSGAAVPGFEL
                     GVPGDLSSAAFLVAAAVLAEGGELLVERVGVNPTRTGFLAVLERMGAHVERLHLGGEG
                     GEPVADLLVRPATLRGTEVGAAEIASLVDEVPVLAVLASRAAGETVFREVGELRVKES
                     NRLELVAANLRAVGVAAEVRGNDLCVRGADHPPRGRVDTARDHRLAMAFAVLGTLPGA
                     EVGLSERASVAISYPRFFTDLKRIGDTGRGTRDE"
     gene            3556..>4236
                     /gene="cmk"
                     /locus_tag="AC16JC_11940"
     CDS             3556..>4236
                     /gene="cmk"
                     /locus_tag="AC16JC_11940"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019826776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0036431 - dCMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HIR5165450.1"
                     /translation="MTPRVIAIDGPAASGKSSTAALVARRLGWAQLDSGALYRALTLA
                     ALDQLGEEGRGKGEGWLAQRILALANELPVRLVLAGNVYRPEVAGVDVEGEIRSERVT
                     ARVSEVAAIPAVREWVNARQREAVAEHPEGVVVDGRDIGTVVFPDAPLKIFLTAAPEE
                     RARRRLNQRGAGMDEERVARESQVLAARDRVDASRPVAPLRPAADAVLLDSTTLSLEE
                     QVARVVVLG"
BASE COUNT          646 a         1389 c         1510 g          691 t
ORIGIN      
        1 cccctcacgg ctgatcagct ccttgcgggt gccgaagcgg acctgcatgc cggcgcggtc
       61 gaggccaacc cgaaccgggc gaatgcgctc gccctgctga gccatctgct ggctcgcaaa
      121 tctgagacag tggatgcgaa gctgacggca atgcaggcct acgaggtgga tccctacctc
      181 tcggacgccg agggagtgct cgggcgactg actctcgcgt cgctcgatct cgaggatgca
      241 gcggagacga acaagtggtg ccaggaaggc taccggcgct ttccgcagag cccgcaattc
      301 acggagtgcc agattcaggt gcacgccctg aaggggcaga agccggatgt gccgcagttg
      361 tggcgcctgc tcgatcagga cgtggcgcag tacccgccgt cggaccgcga cttccgccgt
      421 cgccggggtt ccttgttcgt cgccatggcg ctggccaacg tcggcctcaa ggacagcgcc
      481 cgggccgtgg ccctgcgcgc gcgggccggc gcggacgtgg acccgagccg cgatctcatc
      541 tatgtcgaga ttctgttgcg caatgttttg ggcgaccgcg acgaggcgat tcgactgctc
      601 aagctctacc tcgtcaccaa tccgcaagac aaggcgacca tcgccagcga cagtacgtgg
      661 tggtggcgcg gcctgcgtgg cgatgccgag ttccgacagc tcgtcggcct gaaataggtg
      721 tggccatcgc gccgcactgt cgctgcgttg cagcgatact ctgccgtggc gcgaactctg
      781 ctgtgattct tcacacttaa tcgctggcca tgtgctacct tcagggcgat tcacaatacc
      841 cgaagacaat cgattgacca ccggagtgcg gttcgccgca cgggagggaa gtgcatgcgt
      901 cttcacgtgg gtggtatcgg catcgcagtc ttggcagtgg ccgccatcgg atgtagcgac
      961 cgcagccaac ctctcccggc cggaccgcgc gtgcaggccg tcgtaacggc gaccagcgcg
     1021 ccgccgtgtg atttcaagac cctgagccag ctcgccacac actactttgg ctccgtagaa
     1081 gcgaaggtcg tgcgcggcat catctcgcgg atgcaggact ccggcgcgtt cagcctgacg
     1141 gcgaagaatg gcggcttcga cgtgatgacc cacatcgccg cgaacgtgaa agcgggcaac
     1201 acgggcgtgg ccgacgcttc gaacatgacc aacgggcttc tggcctgcat gttcaacaac
     1261 ccggccgatc tcccgccgac gttcccggag gatttcacga tcgccacgaa cccggcgctc
     1321 cacggcgcct atgacgttcg cggcggcgcg ggcgatttga cgggcccggt cctgagccgg
     1381 ccgcttgacg cgccgctctc gggcgtggcc ccgtctgggt ccaacacctg gcccggcgtc
     1441 ctgtcgggga acccagcgcc ggcccgcgtg ctgatctacg gccaacccgg cctggttccc
     1501 aacacctttg actggcgcgc cgcacccttc tccgtgaact tctcgccggc ggtgatcgtt
     1561 ggggtctgcc tcgacgcgaa ggcgaacgcg acctccctga ttcacgagga gcatgtcggc
     1621 ctgctgccgt tcgccgacgc ggccttcctc ccgacctgca ccgggctcgc gtcgcaatcg
     1681 tggggttcgc agttcgccag ccgactcgtg cgctggggga tgagcgtgct cggtccgcgg
     1741 ccgctgtccg cgacgacctt cctgaacccg ggcggactcg ccggctcgac gggaagcctg
     1801 cgcagcgagt tcgggccaca gctagtcgat acggtcaccc tgaccttcac ggtgcagccg
     1861 agcgacgtca aggtgaactc attcatcacg ccggcggtgg tagtgagggc gacggccgtc
     1921 acggacttcg gcatcgactc ggtgccgaac gtgtccatca cgctctcggc cttcaacaac
     1981 aacggtaccc ccgcgatcct caatgggacg ctgacgcaaa cgacgggtgc cgacggaaag
     2041 gcgacgttcg ccgacctctc cgaaacgaag accggcgggt acgtgctcgt cgcgacggga
     2101 acggtcaacg ggcgcgcggc gatcctcgtg ccggcgacga cgtcggctcg attcaacgtc
     2161 aaaccctagg cgattccgtt acggctgatg acgggcggct ctgcggagcc gcccgttttg
     2221 cgtttacggt gcgggagtcg ttgggtactt tccagtccgt gccaatcatc atcgttccat
     2281 gaaggtcagc ggggtcgtcc gcgtcccggg ggacaagagc atctcacacc gggtgctcat
     2341 tctcgccgcc atggcccgcg gcgagagcac cctgaccggc ctgctcgacg gagcggacgt
     2401 gaagagctca gcccgcattc tgcggcaact cggcgccgac gtgtcgccgc tgcgggaagg
     2461 ggcggtggtg cgcgtgcggg gccggcgatt ccggtctccg caaacgcggc tcgactgcgg
     2521 taactcggga actaccgcgc gtctctgcat cggcgccatc gccgggcacc ccatccgggc
     2581 acagctgacg ggagacgcgt cgttacgccg ccgcccgatg cgacgcgtga tcgagccatt
     2641 gcgccagatg ggggcccggt tcgtcccggt cgagaccgac acgctgccgc tcatggtgcg
     2701 gggcggccgg ctgcacggtc tcacctacgc gtctccggtg gcgagcgcgc aggtgaagag
     2761 cgccatcctc ctcgcggcgt tggcgggacg cgtcccggtg acgatcaccg aaccgtggcg
     2821 ttcgcgcgat cacaccgagc ggttattcgt gcacctcggg ctggagctgc acgagaccga
     2881 cggggccatc agctatcggc cttcaggtgc ggccgtgccc ggattcgagc tcggcgttcc
     2941 cggcgacctt tcctctgcgg cctttctggt cgcggcggcc gtgctggccg agggtgggga
     3001 gctgctcgtc gagcgcgtgg gtgtgaatcc gacgcggacc ggctttctgg ccgtactcga
     3061 gcggatgggc gcgcacgtcg agcggctgca cctcgggggt gaaggtggcg agccggtcgc
     3121 ggacctgctg gtgcggccgg cgacgctgcg cggcaccgaa gtcggggcgg cggaaattgc
     3181 ctcactcgtc gacgaggtac cggtgctggc ggtgctggcg agccgcgccg ccggggagac
     3241 ggtgtttcgg gaggtcggag agctgcgggt aaaggaaagc aaccgcctcg agttggtcgc
     3301 cgcgaatttg cgcgccgtcg gggtcgccgc cgaagtgcgc ggcaacgatc tgtgcgtgcg
     3361 cggtgcggac catccgccgc gcgggcgggt cgataccgcc cgggatcacc ggctggcgat
     3421 ggcgttcgcg gtactgggga cgctgccggg agcggaggtg ggactctcgg aacgcgcgtc
     3481 cgtcgcgatc agctatccgc gattcttcac cgatctcaag cgaatagggg acacgggacg
     3541 cgggacgagg gacgagtgac gcccagagtg attgcgatcg atggcccggc cgcctcgggg
     3601 aagtcgtcga cggcggcgct ggtcgcccgg cggttggggt gggcgcagct cgactcgggc
     3661 gcgctttacc gggccctgac tctcgccgcg ctcgatcagc tgggggaaga gggaaggggg
     3721 aagggggaag ggtggctcgc acagcggatt ttggcgttgg ccaacgagct gcccgtgcgg
     3781 ctcgtcctgg cgggcaacgt ctaccggccc gaagtggccg gcgtggacgt cgaaggggaa
     3841 atccgctccg agcgggtgac ggcgcgggta tcggaggtgg ccgcgatccc cgccgtgcgc
     3901 gaatgggtaa acgcccggca gcgggaggcg gtcgcggagc atccggaggg tgtggtcgtg
     3961 gacggccgcg acatcggcac cgtggtcttt cccgacgccc cgctcaagat cttcctgacc
     4021 gcggcgccgg aggagcgggc ccgccgccgc ttgaaccagc gcggcgccgg aatggacgaa
     4081 gagcgcgtcg cgcgcgagag ccaggtgttg gccgcccggg accgggtgga cgcgagccgc
     4141 cccgtagcgc cgctgcgacc cgcggccgac gccgttctgc tcgattccac cacgctgtcg
     4201 ctggaagagc aggtggcgcg cgtcgttgtg ctgggg
//
LOCUS       DBNGZW010000508         5476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1209030, whole genome shotgun sequence.
ACCESSION   DBNGZW010000508 DBNGZW010000000
VERSION     DBNGZW010000508.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5476
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1209030"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(128..535)
                     /gene="rplS"
                     /locus_tag="AC16JC_11945"
     CDS             complement(128..535)
                     /gene="rplS"
                     /locus_tag="AC16JC_11945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006589897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR5165451.1"
                     /translation="MEKLRAIEREGLKDKLPAFDPCDTVRVMVRVREGEKERLQAFEG
                     VCIARRGGGINETFTVRKISAGVGVERIFPLHAPSIAEIELVRKGRVRRAKLYFLRRL
                     AGKAARIREKRVAVPGSAAPAETAAPPAESPEA"
     gene            complement(557..1258)
                     /gene="trmD"
                     /locus_tag="AC16JC_11950"
     CDS             complement(557..1258)
                     /gene="trmD"
                     /locus_tag="AC16JC_11950"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR5165452.1"
                     /translation="MAVNVVTLFPGWFAAPLGESILGRAAERGLARYRVVQLRDYTHD
                     RHQTVDDAPYGGGAGMVLKPEPFFEAVEDLGLSTGSPIVLLSARGRPFGHRDAVRFSV
                     GAELTLLCGHYKDVDQRVADGLGAEELSIGDFVLSGGEPAALCVIDAVVRLLPGALGD
                     HESAAGDSHYDGPLAAPSYTRPPVYRGLAVPEVLLSGDHEKIAAWREAEARRLTRERR
                     PELWQRLTESEASDA"
     gene            complement(1248..1472)
                     /locus_tag="AC16JC_11955"
     CDS             complement(1248..1472)
                     /locus_tag="AC16JC_11955"
                     /inference="COORDINATES: protein motif:HMM:NF013907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HIR5165453.1"
                     /translation="MVPLVEIPGAVLQPKAQLWIVDEDRRVLAGPLVLTRRRAYHREW
                     LIGFAGLTSRDAVDGWRDRFLAVPEDDAGG"
     gene            complement(1517..1774)
                     /gene="rpsP"
                     /locus_tag="AC16JC_11960"
     CDS             complement(1517..1774)
                     /gene="rpsP"
                     /locus_tag="AC16JC_11960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011735027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR5165454.1"
                     /translation="MATRIRLRRVGRKGQSYFRIVIADVRAARDGRSVATIGHYDPRA
                     NPAVLEVDKDKASAWLAKGAMPTDTVRSLLKKAGVFGSRTA"
     gene            complement(1786..3081)
                     /gene="ffh"
                     /locus_tag="AC16JC_11965"
     CDS             complement(1786..3081)
                     /gene="ffh"
                     /locus_tag="AC16JC_11965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006904994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR5165455.1"
                     /translation="MFTALTERFSGVVQRLVGRGVLSEQDVAEALRDIRRALLEADVS
                     LEIARDFVERVRARAVGTVQLKTVRPGEQVVKLVHDELAGLLGGTRHDLEFSTVGPTV
                     ILLVGLQGSGKTTTAAKLARRLKLEQKAPGLVAADFQRPAAVEQLKQLGEQVGVPVLG
                     TGDEGRGMSVVERVRASVREAEKARCRTVIIDTAGRLQIDADLMAELKALKEAVRPRA
                     VLLVADAMTGQDAVRIARGFQEGVGLTGVILTKLDGDARGGAALSIHGATGAPIVYVG
                     VGERPDAFEPFDPGRMAGRILGRGDVVALVEKAAATIDQEAAQRLEKKARSKRGMDLQ
                     DFLVALKQMQKMGPLKQMLGLLPGVPAKALKALPADDRRLKHVEAIVLSMTPEERADP
                     KVLNGSRRLRVAKGSGRPVQEVNRLLEQFQQMRKFLQRM"
     gene            complement(3136..4359)
                     /locus_tag="AC16JC_11970"
     CDS             complement(3136..4359)
                     /locus_tag="AC16JC_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165456.1"
                     /translation="MGRLTSAAAALLVVAGGCRERAKVVESETATRDLVRRLAVQASQ
                     VTGLHFKSPVAVRLRTREQVRAYIIHKFDQDLPPPELAGVQAAYRLFGLIPDSLDLRR
                     TYVDLLTEQVAGYYDPDSSALFIPTDQGDPAQVRLVASHELVHALQDEYLPLDSIINQ
                     RRQNDRRSAAQAVLEGQATFYQISILMPEQHPESLPPGWFWRQRSAMALQQAAMPAFS
                     SAPMWLRETLIFPYLGGADFVSWFARTHPRREPYGAAMPASTEQILHPDRYAAGDQPV
                     ALAFSGPAVDSVRFEDGLGEFEIGLLITQLRGDSTSDAGRAAARGWGGDRYRVYGLNA
                     DALVWYSVWDDAGARDNFLRSLEPAWAARRSGDATRRFRVESLTIDDRPGVRLVDAPP
                     SWPGWRTLPEVKVLP"
     gene            complement(4347..>5476)
                     /locus_tag="AC16JC_11975"
     CDS             complement(4347..>5476)
                     /locus_tag="AC16JC_11975"
                     /inference="COORDINATES: protein motif:HMM:NF012234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5165457.1"
                     /translation="FESGDVLERVGVDMTALARRGDLEPVRCRDAEIARVVDILLRAS
                     KNNPALVGKAGVGKTAIVEGLAQRIAAGDVPPALKDARIVALDHVALLAGTSFRGQYE
                     ERIRRLVAALAADPDLVLFVDELHNLIGQGTAMGAAMDAANMLKPALVRGDIRVVGAT
                     TSEEYERWIRHDPALERRFQPLTILELDAIQTWEVLVARRPRLERHHAVAITDDALKA
                     AIVLTDQFVPERARPDKAIDVLDEACAHAQATARVSPELDRLIRERRKVDARLRRESA
                     GGAPADGGEEVLPDLASMFERIGRELEAMLGGERRAAAPPGGETVSTAPPRRAPSLQE
                     QRDELDRLLRAELERQGIVVGGADVARVVAAAAGKGIEWAG"
BASE COUNT          819 a         2109 c         1764 g          784 t
ORIGIN      
        1 aacacgacgg ccgcggcgac caccggtcct gccagtgggc cgcggccgac ttcgtcgatg
       61 ccggcgacga tcgtccgccc cgcgttccag agcgcggctt cgcgctcgaa cgtggggcgg
      121 cggccgctca cgcctcggga ctctccgcgg gcggtgcggc cgtttcggcc ggggcggcac
      181 tgcccggcac cgcgacccgc ttctcccgga tacgcgccgc cttgccggcg aggcgccgca
      241 ggaagtagag cttggcgcgc cgcacgcgac ccttgcgcac cagctcgatc tcggcgatcg
      301 aaggcgcgtg cagcgggaag atgcgctcca cgcccacacc cgccgagatc ttgcgcaccg
      361 tgaacgtctc gttgatgccg ccgccgcggc gcgcgatgca cactccctcg aacgcctgga
      421 ggcgctcctt ctcgccctcg cgcacgcgga ccatcacccg caccgtgtca cacggatcga
      481 acgccgggag cttgtccttc aggccttcac gctcgatcgc tcgcagcttc tccatacccg
      541 taccctctcg tgacgtctac gcgtcgctgg cttccgattc ggtcaaccgc tgccacagct
      601 cgggacgccg ctcgcgcgtc agccggcggg cttctgcctc acgccacgcc gcgatcttct
      661 cgtgatcccc gctcaacaat acctcgggaa ccgccaaccc gcgatacacc ggcggccgcg
      721 tgtaagacgg cgcggccagt ggaccatcgt aatgcgaatc tcccgccgcg gattcgtgat
      781 cgcccagcgc ccccggcagc aggcgcacga cggcatcgat cacgcacagc gccgccggct
      841 cgccgcccga caggacgaag tcgccgatcg acagctcctc ggcccccagg ccgtcggcca
      901 cgcgctggtc cacgtccttg taatgcccgc acaacagcgt caactccgct cccaccgaga
      961 accgcaccgc gtcccggtga ccgaagggac ggccccgtgc cgacagcagt acgatcggcg
     1021 atcccgtcga gaggcccagg tcctccaccg cctcgaagaa cggctccggc ttcagcacca
     1081 tcccggcgcc cccaccgtag ggggcgtcgt ccaccgtctg gtgccggtcg tgggtgtagt
     1141 cccgcagctg caccacgcgg tagcgcgcga gaccccgttc ggccgcccgc cccagaatgc
     1201 tctctcccag cggcgccgcg aaccagccgg gaaacagcgt cacgacgtta accgccagca
     1261 tcgtcctcag gtaccgcgag aaaccggtcc cgccacccgt ccaccgcgtc ccgcgacgtc
     1321 aggcccgcga atccgatgag ccattcgcgg tgataggccc gccgccgcgt gagcacgagc
     1381 ggtccggcga gcacccgccg atcctcgtcc acgatccaca gctgcgcctt cggttgcagc
     1441 accgcgcccg ggatctccac gagaggcacc accgacacct ccccgtgttg cccgtgcggc
     1501 ttgcgcaacc gcgcgactat gcggtacgtg acccgaacac cccggccttc ttgagcagcg
     1561 accggaccgt gtcggtcggc atcgcccctt tggcgagcca cgccgacgcc ttgtccttgt
     1621 cgacctcgag aaccgccggg ttggcgcgtg gatcgtagtg gccgatcgtc gcgaccgagc
     1681 gcccgtcgcg cgcggcccgc acatcggcga tcacgatgcg gaagtacgac tgtcccttcc
     1741 ggcccacgcg gcgcagccga atccgagttg ccatgaaatc cgcccttaca tccgttggag
     1801 aaacttgcgc atctgctgga actgctccag cagccggttc acttcctgta cgggccgtcc
     1861 cgagcctttg gccacccgca gccgccgcga cccgttgagg accttcggat cggcccgctc
     1921 ctcgggcgtc atcgacagga cgatcgcctc gacgtgcttg agccgtcgat cgtcggccgg
     1981 cagggccttg agcgccttcg ccggtacgcc cggcagcaag ccgagcatct gcttcagcgg
     2041 tcccatcttc tgcatctgct tcagcgcgac gaggaagtcc tgcagatcca tgccgcgctt
     2101 ggaccgcgcc ttcttctcga gccgctgggc ggcctcctgg tcgatcgtgg ccgccgcctt
     2161 ctccaccagc gccacgacgt cgccccggcc caggatccgg ccggccatcc gcccgggatc
     2221 gaacggctcg aaggcgtcgg gccgctcccc cacgccgacg tacacgatcg gcgctcccgt
     2281 cgcaccgtgg atcgacagcg ccgccccacc gcgcgcgtcc ccgtccagct tcgtgaggat
     2341 cacgccggtc aggccgaccc cctcctggaa cccgcgcgcg atccgcaccg cgtcctgccc
     2401 cgtcatcgcg tcggccacga gcagcaccgc gcgcggccgc accgcttcct tcagcgcctt
     2461 cagctcggcc atcaggtcgg cgtcgatctg caaccggccg gccgtatcga tgatcaccgt
     2521 gcggcagcgc gccttctcgg cctcccgcac ggaagctcgg acgcgctcga ccacactcat
     2581 cccccgtccc tcatcccccg tccccaagac cggcacgccg acttgctcgc cgagctgttt
     2641 gagttgctcg accgccgccg ggcgctggaa atccgcggcg accagccccg gcgccttctg
     2701 ctcgagcttc agccgtcgcg cgagcttggc cgccgtcgtc gtcttgcccg agccctgcaa
     2761 cccgacgagc aggatgaccg tcggaccgac ggtcgagaac tccagatcgt gccgcgtccc
     2821 gcccagcagt ccggccagct cgtcgtggac cagcttcacg acctgctcgc cgggacgcac
     2881 cgtcttgagc tgcaccgtgc cgacggcccg cgcgcgcacc cgctcgacga agtcgcgggc
     2941 gatctcgagg ctcacatcgg cttcgagcag cgcccgcctg atgtcgcgca acgcctcggc
     3001 gacgtcctgc tccgacaaga cgccccgacc gacgagccgc tgcaccacac cggagaatcg
     3061 ctcggtcagg gccgtaaaca taagccacgc aaaatactta gccgacggat ggaaaccaag
     3121 tggggagaaa ccgagctacg gcaggacctt cacctccggc agcgtgcgcc atcccggcca
     3181 ggacggcggc gcgtccacca accggacacc cggtcggtcg tcgatggtga gcgactccac
     3241 gcggaagcgc cgcgtggcgt cgccggaccg ccgcgccgcc caggccggct cgagggagcg
     3301 caggaagttg tcgcgcgccc ccgcgtcgtc ccacacgctg taccacacca gggcatcggc
     3361 gttgagaccg tagacccggt agcgatcccc gccccacccc cgcgcggcgg cccgcccggc
     3421 gtccgacgtc gaatcccccc gcagctgagt gatgaggagc ccgatctcga actcccccag
     3481 gccatcctcg aaccgcaccg aatcgaccgc cggcccggag aaggccagcg ccaccggctg
     3541 gtcaccggcg gcatagcggt cgggatggag gatctgctcg gtggaggcgg gcatcgcggc
     3601 gccgtagggt tcgcgccgcg gatgcgtgcg ggcgaaccag gagacgaagt ccgcaccacc
     3661 cagataggga aagatcaggg tctcccgcag ccacatcggc gcgctggaga aggcgggcat
     3721 ggccgcctgc tgcagcgcca tggccgaccg ctgccgccag aaccaccccg gcggcaggct
     3781 ctccggatgc tgctcgggca tgaggatcga gatctgatag aacgtggcct ggccttcgag
     3841 caccgcctgc gccgcggaac ggcggtcgtt ctgccgtcgc tgattgatga tcgagtcgag
     3901 cggcagatac tcgtcctgca acgcgtgcac cagctcgtgt gaggcgacga gacggacctg
     3961 cgcggggtcg ccctgatcgg tcgggatgaa caaggccgac gagtcggggt cgtaatagcc
     4021 cgccacctgc tcggtgagca ggtccacgta cgtgcgacgc aggtcgagtg aatcgggaat
     4081 cagaccgaac agccggtatg ccgcttgcac gcccgccagc tcgggtggcg gaagatcctg
     4141 gtcgaacttg tggatgatgt aggcccggac ctgttcgcgc gtgcgcaggc gcaccgccac
     4201 gggactcttg aagtgaagtc cggtcacctg ggaggcctgg acggcgagcc ggcggaccag
     4261 atcgcgcgtt gcggtctcgc tctccacgac cttcgcccgc tcgcggcagc caccggccac
     4321 gaccagcagc gcggcggctg cgctcgtcag ccggcccact cgatcccctt ccccgcggcc
     4381 gccgcgacga cgcgcgcgac atccgctccg ccgaccacga tcccctgtcg ctcgagctcg
     4441 gcgcgcagca accggtccag ctcgtcgcgc tgctcctgca acgacggcgc gcggcgcggc
     4501 ggtgcggtgg acaccgtctc gccgccgggc ggcgcggcag cgcgccgctc tccacccagc
     4561 atcgcctcga gctccctgcc gatccgctcg aacatgctcg cgagatcggg gagcacttcc
     4621 tcgccgccat cggccggcgc tccccccgcg gactcgcgcc gcaaccgtgc atccaccttg
     4681 cggcgctcgc ggatcagccg atccagctcg gggctgacgc gtgccgtggc ctgggcgtgc
     4741 gcgcaggctt cgtcgagcac gtcgatcgcc ttgtcgggcc gggcccgctc gggcacgaat
     4801 tggtcggtga gcacgatggc cgccttgagg gcatcgtcgg tgatcgccac ggcgtggtgc
     4861 cgctcgagcc gtgggcgccg cgcgacgagc acctcccagg tctggatcgc gtcgagctcg
     4921 aggatggtga gcggctggaa gcggcgctcc agcgccggat cgtgacggat ccagcgctcg
     4981 tattcctcgc tcgtggtcgc ccccacgacg cggatgtcgc cccgcaccag cgccggcttg
     5041 agcatgttgg ccgcgtccat ggcggccccc atggcggtcc cctgcccgat gaggttgtgc
     5101 agctcgtcca cgaacaggac gagatcgggg tcggcggcga gcgctgcgac gagtcggcga
     5161 atgcgctctt cgtactggcc gcggaaggag gtgcccgcga gcagcgccac gtgatcgagg
     5221 gcgacgatgc gggcatcctt gagggcgggc ggcacgtccc cggcggcgat gcgctgggcc
     5281 aggccttcca cgatggcggt cttgcccacc cccgccttgc cgacgagcgc ggggttgttc
     5341 ttgctcgcgc gcagcaggat gtccaccacg cgcgcgatct ccgcgtcgcg gcagcggacg
     5401 ggctcgaggt cgccgcgccg ggcgagcgcg gtcatatcga ccccgacgcg ctcgagcacg
     5461 tcgcccgact cgaaca
//
LOCUS       DBNGZW010000509         4568 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1617076, whole genome shotgun sequence.
ACCESSION   DBNGZW010000509 DBNGZW010000000
VERSION     DBNGZW010000509.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4568)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4568)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4568
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1617076"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..445)
                     /locus_tag="AC16JC_11980"
     CDS             complement(155..445)
                     /locus_tag="AC16JC_11980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165458.1"
                     /translation="MIHTATVALAPSEVLARAKRYFAERVPNQAAFVEKEGPQFVVMR
                     GQGGEELVIRAEEGKGEEGRGKSVVRASTLFFDQVLDRFFSTLPGISGVEVA"
     gene            complement(442..1542)
                     /locus_tag="AC16JC_11985"
     CDS             complement(442..1542)
                     /locus_tag="AC16JC_11985"
                     /inference="COORDINATES: protein motif:HMM:NF014312.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR5165459.1"
                     /translation="MRVLRGTALCLAWTVPAVAQAPAPPAAPMYGYRLYVVSESGDIV
                     TQLGWDGTTLKTIKTVPVGVMPADIDGPHNVTVSPDGRFYYVTIAHGTPYGSLWKMAM
                     DGDTLLGRAPVELFPTTISVTPDGELAFVANSDFYGDHPRTNPVSVIYTPRMTPLTNI
                     AACDMPHGVKVNHAGTFVYVSCMNSDEVLEIDRQSLAIARRHRTGGAGMAGRAMGGMT
                     AGAPECMPTFVSLSPDDRRLYVACNHANTLQVIDAATFVLVKEVPTGTGAYNVEPSPD
                     GRWVIVTNKKAQSVSVISADSLVEVARVATTKKIPHGIAYSPDSRWAFVSQESIGVDP
                     GAVDVIDLTTRTRVATVPVPLQPTGITLRVLP"
     gene            1734..2201
                     /locus_tag="AC16JC_11990"
     CDS             1734..2201
                     /locus_tag="AC16JC_11990"
                     /inference="COORDINATES: protein motif:HMM:NF012241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HIR5165460.1"
                     /translation="MYQTTRSYNGDRAGDFATLGNRINRMLNDALNGTDWPYRDSATA
                     AWVPPVDVLEEAGAIRIMAELPGVRPEDVRISVEGNVLTVHGTKQQVAEERTEHVHRY
                     ERTYGAFERTFTLPATVDAEHIKAGYENGVLSITLPKVERAKPRQIPVETSKQ"
     gene            complement(2256..2567)
                     /locus_tag="AC16JC_11995"
     CDS             complement(2256..2567)
                     /locus_tag="AC16JC_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165461.1"
                     /translation="MTEGRRRVHLLTRDLMFRGKLGGVVSAAGGDVTRDPAECDLAVV
                     ELEARGWEDAIRDIHARGVSVLAFGSHVNAGLLRRARELGAEAVPNSQVEGRLAELLR
                     G"
     gene            complement(2564..>4568)
                     /locus_tag="AC16JC_12000"
     CDS             complement(2564..>4568)
                     /locus_tag="AC16JC_12000"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HIR5165462.1"
                     /translation="TAAGQRADSGLPPLRVVEPRWIDKSVNACTNFFQYANGAWLAHD
                     TIPAAYSSSGVTRDMSDRNELVVRSVLEDAAARRSAFPPDSTPRKLGTFYATCMDSTA
                     AEAAGVNPIRPWLAGVDSITTRARLLPQIATLQIRGVNAVFRYYPLADPHDAAHYEVW
                     LLQGGLGLPDRDYYTNSGAAADSLREAYVAHVTRLFTLAGRDPTSSARDAAQVLALET
                     ALARAALTRVQRHDPAATDHPTTTAELRRVAPTIEWPAYFQAIGLRAPVSRLNLAEPA
                     FVRRVDSLIGAAPLEQWRAYLRYHALETAAPWLSTPFVQENFAFRSRFSGAKALLPRW
                     KRCLRETDGDLGEALGQAYVAKTFPPQARARAKAVIDDIRAAFGERLRHLGWMSDSTR
                     AQALDKLARMGEKVGYPDHWRDYSRLAVAAGPFVLNMARANAFEWQRTVNRPGMPVDT
                     TEWDITVPTVNAYYDPTKNEMVFPAGALVPQTFDPEADDGANYGSLGGSWAGHELTHG
                     FDDEGRHYDASGNLRDWWIPADSVHFTQGADLVARQFDGYVQVDTFHVNGRLTLGENI
                     ADYGGVLTGYDALQRALQRDGRPEPIDGYTPEQRFFLGYAQSWRVHDRDEQLRTRVTV
                     DPHAPERWRVNGPLSNVAAFAQAFGCKAGDPMVRPPEQVPQIW"
BASE COUNT          725 a         1596 c         1558 g          689 t
ORIGIN      
        1 tccccgagcg gcggggccgc ctgcttcagc gcccgcgtca gcgcctgccg cttgagctcc
       61 gccaccgtcg tctcgggacc cacggccaga aacacctggt cccaggcgtc ggtcaccatc
      121 acccggacgg cgaagcgttc cgcgatcccc gcggtcatgc gacctccacg ccggagatcc
      181 cgggcagcgt cgagaagaac cgatccagca cctgatcgaa gaacagggtc gaggcacgaa
      241 ccacactctt cccccttccc tcttccccct tcccttcctc cgcccggatc acgagttctt
      301 ctcccccctg cccccgcatc accacgaatt gcgggccctc cttctccacg aacgccgcct
      361 gattcggcac gcgctcggcg aagtaccgtt tggcgcgcgc cagcacctcg gatggtgcga
      421 gcgcgaccgt ggccgtatga atcatggcag caccctgagc gtgattccgg tgggctggag
      481 cggcaccggg acggtggcga ccctggtgcg ggtcgtgaga tcgatcacgt ccaccgctcc
      541 gggatcgacg ccgatgctct cctgactcac gaacgcccac cggctgtcgg gagaataggc
      601 gatcccgtgc gggatcttct tcgtcgtggc gacgcgcgcc acctcgacca gcgaatccgc
      661 ggaaatcacg gatacgctct gcgccttctt gttcgtgacg atcacccacc ggccgtcggg
      721 tgacggctcc acgttgtagg cgccggtgcc ggtggggacc tccttcacga gcacgaacgt
      781 cgcggcgtcg atcacctgca gcgtgttggc gtgattgcag gcgacgtaga gacgcctgtc
      841 gtccggcgag agcgacacga acgtcggcat gcactcgggg gcgcccgccg tcatcccacc
      901 catggcccgc ccggccatcc ccgccccccc cgtccggtgg cgccgggcga tcgcgagcga
      961 ctgccggtcg atctccagca cctcgtcgga gttcatgcag ctgacgtaca cgaacgtgcc
     1021 ggcatgattc accttcaccc cgtgcggcat gtcgcaggcg gcgatgttgg tgagcggcgt
     1081 catccggggc gtgtagatca ccgaaacggg gttggtgcgc ggatgatcgc cgtagaaatc
     1141 cgagttcgcc acgaacgcca gctcgccgtc gggcgtgacc gagatcgtgg tggggaacag
     1201 ctcgaccgga gcccgaccca gcagggtgtc tccgtccatg gccatcttcc acagcgagcc
     1261 gtacggcgtc ccgtgcgcga tggtcacata atagaagcgg ccgtcgggag acaccgtgac
     1321 gttgtggggg ccgtcgatgt cggccggcat gacccccacc gggacggtct tgatggtctt
     1381 gagggtggtg ccgtcccagc cgagctgcgt gacgatgtcc ccggactcgc tgaccacgta
     1441 cagccggtag ccgtacatgg gggcagcagg tggcgcggga gcttgagcca cggccggcac
     1501 cgtccaggcg aggcagagtg ccgtaccccg caggacgcgc atgcgatcca aaggaatgct
     1561 ccagagtggg tagcccggca aaactgacag tcggcaatgc caacatgatc ataacacgtt
     1621 gccaactcgg catctaggag gcctccccga gcggcacggt ggttgccaag cccctgaccg
     1681 gcgaggcggg tgacctcgaa aaccagaacc aattgtccga aggaggcatg tgtatgtacc
     1741 agaccacgcg cagctacaat ggggaccggg ccggggattt cgcgacgctc gggaatcgga
     1801 tcaaccggat gctgaacgac gcgctcaacg ggacggactg gccgtaccgc gacagcgcca
     1861 cggccgcctg ggtcccgccg gtggacgtgc tggaggaagc cggcgcgatc cgcatcatgg
     1921 ccgagctccc cggcgttcgg cccgaagacg tcaggatttc ggtcgagggc aacgtgctca
     1981 ccgtccacgg caccaagcag caggtggccg aggagcggac cgagcacgtg caccgctacg
     2041 agcggacgta cggcgcgttc gagcgcacct tcaccctgcc ggccacggtg gacgccgagc
     2101 acatcaaggc cggctacgag aacggcgtgc tttcgatcac cctgcccaaa gtcgagcggg
     2161 cgaagccgcg gcagatcccg gtcgagacgt caaaacagta acggctggtg atgtgagcac
     2221 gtggacggcc ccggcatccg gggccgttcg tcgtttcagc cgcggagcag ctccgccagc
     2281 cggccttcca cctgcgagtt ggggacggcc tcggctccga gctcccgggc gcgccgcagc
     2341 agcccggcgt tcacgtgcga gccgaatgcc agcacggaga caccgcgggc atgaatgtcg
     2401 cggatggcgt cttcccatcc ccgcgcctcg agctcgacca cggccaggtc gcactcggcg
     2461 gggtcgcgcg tcacgtcccc ccccgcggcg ctcaccacac ccccgagctt cccgcgaaac
     2521 atcagatcgc gggtgagcag atggacgcgg cgcctcccct cggtcaccag atctggggca
     2581 cctgctctgg cggccgcacc atcgggtcgc ccgccttgca gccgaacgcc tgcgcgaacg
     2641 cggccacgtt ggagagcggg ccgttcacac gccaccgctc cggcgcatgc ggatccacgg
     2701 tgacgcgcgt gcgcagctgc tcgtcgcgat catggactcg ccagctctgc gcgtagccaa
     2761 ggaagaaccg ctgctcgggg gtgtagccgt cgatcggttc gggccggccg tcgcgctgca
     2821 gcgcgcgctg gagcgcgtcg tacccggtca gcacgccgcc gtagtcggcg atgttctcgc
     2881 ccagcgtgag cctgccgttc acgtggaacg tgtccacctg gacgtagccg tcgaactggc
     2941 gcgcgaccag gtccgccccc tgggtgaaat ggacggaatc cgcaggaatc caccagtcgc
     3001 gcaggttgcc gctcgcgtcg tagtgccgcc cttcgtcgtc gaagccgtgc gtcagctcgt
     3061 gccccgccca gctgcctccc agggagccgt agttcgcccc atcgtcggcc tccggatcga
     3121 aggtctgcgg taccaaggcc cccgcgggaa acaccatctc gttcttggtc ggatcgtagt
     3181 aggcgttcac ggtggggacg gtgatgtccc attccgtcgt gtccaccggc atgcccgggc
     3241 gattcaccgt gcgctgccat tcgaaggcgt tggcgcgggc catgttcagg acgaacggcc
     3301 ctgcggcgac cgcgagccgc gagtaatcgc gccagtggtc ggggtagccc accttctcgc
     3361 ccatgcgggc gagcttgtcg agcgcctggg cgcgggtcga gtccgacatc cagccgaggt
     3421 gacgcagccg ttccccgaat gcggcgcgga tgtcgtcgat tacggccttg gcgcgagccc
     3481 gcgcctgcgg cgggaacgtc ttggcgacgt acgcctggcc gagggcttct ccgagatcgc
     3541 cgtcggtctc gcgcaggcac cgcttccagc gcggcagcag cgccttggcc ccgctgaagc
     3601 gcgagcggaa cgcgaagttc tcctgcacga acggcgtgct gagccagggc gcggcggtct
     3661 cgagcgcgtg atagcgcagg taggcgcgcc actgctccag cggagccgct ccgatcagcg
     3721 agtcgacccg ccgcacaaag gccggctcgg ccaggttgag ccgcgacacg ggggcgcgca
     3781 gtccgatcgc ctggaagtag gccggccatt cgatcgtcgg ggcgacccgg cgcagctcgg
     3841 cagtcgtcgt cgggtggtcg gtcgccgcgg gatcgtggcg ctgcacgcgg gtcagcgccg
     3901 cacgggccag cgcggtttcg agcgccagca cctgcgcggc atcgcgggcg gacgacgtgg
     3961 gatcccgtcc cgcgagcgtg aacagccggg tcacgtgcgc cacgtacgcc tcccgcaggg
     4021 agtccgccgc cgcgccgctg ttcgtgtagt agtcgcgatc cgggagtccg agaccgccct
     4081 ggagcagcca cacctcgtag tgcgccgcgt cgtgcgggtc ggccagcggg tagtagcgga
     4141 acacggcgtt gacgccacgg atctgcagcg tggcgatctg cggcagcagg cgcgcacggg
     4201 tggtgatcga atccaccccg gcgagccagg gccggatcgg gttcaccccg gccgcctccg
     4261 cagcggtgga gtccatgcac gtcgcgtaga acgtaccgag cttccggggc gtgctgtcgg
     4321 gagggaacgc gctgcggcgc gccgccgcgt cctccagcac cgagcgcacc accagctcgt
     4381 tgcggtcgct catatcgcgc gtcacgcccg aggatgagta ggccgcggga atcgtgtcgt
     4441 gggccagcca cgcgccattg gcatactgaa aaaagttggt gcacgcgttg acggacttgt
     4501 cgatccagcg cggctccacc acgcgcagcg ggggcagccc ggagtcggcg cgctgtccgg
     4561 cggctgtg
//
LOCUS       DBNGZW010000510         2006 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1399391, whole genome shotgun sequence.
ACCESSION   DBNGZW010000510 DBNGZW010000000
VERSION     DBNGZW010000510.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2006)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1399391"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="AC16JC_12005"
     CDS             complement(<1..871)
                     /locus_tag="AC16JC_12005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5165463.1"
                     /translation="MPDTTRSAPSLEAHWMPFTANRAFKRAPRLLVSASGMHYTAADG
                     RRILDGTAGLWCVNAGHAPEPIVRAIAESAARLDYAPGFQMGHPGSFALAERLGTLLP
                     GDLDRVFFTNSGSEAVDTALKIALAYFQARGEPQRARFVGRQRGYHGVGFGGISVGGL
                     ESNRKAFAAQLLPLVDHLPHTHDLAHNAFSRGQPGWGAELADALEAIVTQRGAETIAA
                     VIVEPVAGSTGVLVPPRGYLERLRALCDRHGILLILDEVQSGVGRTGRMFACQHWEVA
                     PDIMTLAKGLGSGL"
     gene            complement(880..>2006)
                     /locus_tag="AC16JC_12010"
     CDS             complement(880..>2006)
                     /locus_tag="AC16JC_12010"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5165464.1"
                     /translation="SSGTKKAIALVNHLDVVPVTPQFWSVDPFSGAVRDGYVWGRGAL
                     DMKGEGMMQLMAMIALKRSGVPLARDIVFIGNADEELGGTGAMTFVKRHADLLSDVEF
                     LMTEGGDDLVNNGHVDYYGVGVAEKRTFWQRITVRGTPSHGSRPTKLNPVPHLVDALS
                     RLAHYETPLHVTPGVAKYFNDIAPQYGGRQRAWLSDISAALADSAARAWVTDNVYWNA
                     ILRNTISLTVLSASNKTNVIPAEASAEIDVRLLPDQDTAVVRAELVRVVGDTAVHWQT
                     LLPPKAPLESPIDTDLFRAIERAARERDPAARVTTPMLTGATDRPTYRALGIVTYGFD
                     PFRVEKADAQRGVHGNDERVSVANVSFGVRYLYDVLRYAQ"
BASE COUNT          298 a          711 c          699 g          298 t
ORIGIN      
        1 gcaggccgga gccgaggccc ttggccaggg tcatgatgtc cggcgccacc tcccagtgct
       61 ggcaggcgaa catcctgccc gtcctcccca cgccggactg gacctcgtcc aggatcagca
      121 ggatcccgtg ccggtcgcac agcgcgcgca gccgctcgag gtagccgcgc ggagggacca
      181 gcacgccggt cgagccggcc accggctcca cgatcaccgc cgcgatcgtt tccgcgccgc
      241 gctgcgtcac gatcgcttcc agggcatcgg cgagctccgc cccccacccg ggctgcccgc
      301 gggagaaggc gttgtgtgcg agatcgtgcg tgtggggcag gtggtcgacc agcgggagga
      361 gctgcgccgc gaacgccttg cggttgctct cgagcccgcc gaccgaaatc ccgccgaagc
      421 ccacgccgtg gtagccgcgc tggcgtccca cgaaacgcgc ccgctgcggc tccccgcgcg
      481 cctggaaata ggcgagcgcg atcttgagcg ccgtgtccac cgcctcggag ccggagttgg
      541 tgaagaacac gcggtccaga tcgcccggca gcagcgtacc gagccgttcg gccagcgcga
      601 agctgccggg gtggcccatc tggaacccgg gcgcgtagtc gagccgcgcc gcggactccg
      661 cgatggcgcg gacgatcggc tccggcgcgt ggccggcgtt cacgcaccac agccccgccg
      721 tgccgtccag gatccggcgc ccgtccgccg ccgtgtagtg catcccgctg gccgagacga
      781 gcaggcgcgg ggcacgcttg aacgcccggt tcgccgtgaa gggcatccaa tgcgcttcga
      841 gcgacggcgc ggaacgcgtg gtgtccggca tggtgactcc tattgcgcgt agcgcagcac
      901 gtcgtacagg tagcgcacgc cgaagctcac gttcgcgacg ctcacccgct cgtcgttgcc
      961 gtggacaccg cgctgcgcgt ccgccttctc cacccggaag ggatcgaacc cataggtgac
     1021 gatccccagc gcccggtaag tggggcggtc ggtggccccg gtgagcatcg gcgtggtcac
     1081 ccgcgcggcc gggtcgcgct cgcgggccgc ccgctcgatg gcgcggaaca gatccgtatc
     1141 gatcgggctc tcgaggggcg ccttgggcgg caggagggtc tgccagtgga ccgcggtgtc
     1201 gcccacgacg cgaaccaact ccgcgcgcac cacggccgta tcctggtcgg ggaggagccg
     1261 gacgtcgatc tcggccgacg cctcggcggg gatgacgttc gtcttgttcg aagcgctcag
     1321 gacggtgagg gaaatggtat tccgcaggat cgcgttccag tagacgttgt cggtgaccca
     1381 ggcgcgggcc gcggaatccg ccagcgccgc ggagatatcc gagagccagg cgcgctgccg
     1441 gccgccgtat tgcggggcga tgtcgttgaa gtacttggcg acgccggggg tgacgtgcag
     1501 cggcgtctcg taatgggcca ggcgcgacag ggcgtccacg agatgcggca cggggttcag
     1561 cttggtgggt cgcgagccgt gcgagggtgt cccgcgcacc gtgatgcgct gccagaacgt
     1621 acgtttctcc gccacgccga cgccgtagta gtcgacgtgg ccgttgttga ccaggtcgtc
     1681 tccgccctcg gtcatcagga actcgacgtc gctgagcaga tccgcgtgcc gtttcacgaa
     1741 cgtcatcgcg ccggtgccgc ccagctcctc gtccgcattg ccgatgaaca cgatgtcgcg
     1801 agcgaggggc acaccgctgc gcttgagcgc gatcatggcc atcagctgca tcatcccctc
     1861 gcccttcatg tcgagcgccc cacgccccca cacatagccg tcgcgcaccg ctcccgagaa
     1921 cgggtccacc gaccagaact gcggggtcac cggcaccacg tccaggtgat tcacgagcgc
     1981 gatcgccttc ttcgtgccac tggagc
//
LOCUS       DBNGZW010000511        19353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_320049, whole genome shotgun sequence.
ACCESSION   DBNGZW010000511 DBNGZW010000000
VERSION     DBNGZW010000511.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 19353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 19353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19353
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_320049"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="AC16JC_12015"
     CDS             <1..851
                     /locus_tag="AC16JC_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017889269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5165465.1"
                     /translation="LHCDITLQAAQAGKHVVIEKPLCMNLAEADRMIDGCRAAQVKLM
                     YAEELCFAPKYVRLKGLLDEGALGKPVLLKQSEKHDGPHAEHFWDVERSGGGVTMDMG
                     CHAIEFFRWLNGNTPLRSVYAQMSTSVHTAKTKGDDNAIIILEFENGVIAVAEESWTK
                     LGGMDDQAEIHGSEGVAYADLLHGNAIETYSTRGVGYAVEKAGITRGWSFTMYEEVWN
                     YGFPQELEHFVHCVKHDETPRVTGEDGRAVLETIFAAYESAGTGRKVTMPFVSDADRP
                     YKLWRK"
     gene            929..1969
                     /locus_tag="AC16JC_12020"
     CDS             929..1969
                     /locus_tag="AC16JC_12020"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF014477.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HIR5165466.1"
                     /translation="MTPQTPDLPASRIGALTSLGFEQVQPSVCETAVHDAGHDGFRVL
                     SRRAAMSTLVDIVAIHQSAGLAEEAIGRAFEEMDRLVAIFNRHDRASALSLLNSEGHL
                     EDPPPELALVLSDALNCHRVSDGAFDPTVAPLVDLFQACFTGPTPHEPTAAEIAEARG
                     LVSAGAITASPRRVRLTRRGTTLTLDGIAKGFIVDRMAATLEGAGVHRYLVEAGGDIR
                     TGGRKEDRLPWAVAIRDPESGGRGRLPGTIQLSGAAVATSGSYERYYDPERRFHHIID
                     AARGSSPHDCVSATVIAPTATAADALATAVLVLGSRAGKALVERLPGCACHIVRSDGR
                     VTHSHGWKESPA"
     gene            1966..2475
                     /locus_tag="AC16JC_12025"
     CDS             1966..2475
                     /locus_tag="AC16JC_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020532433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HIR5165467.1"
                     /translation="MTRPQSATLVTLRLLVGWHFLYEGLAKLANPYWTSAEYLDQAGW
                     LFKKLFVALAASPSAVTVVDFLNEWGLVLIGLGLLVGLFTRTATIAGIVLLLLYYIAA
                     PPLVGISSAVPREGSYLLVNKVLIEAAALAVLLAFPTGRSFGLDGLIFRRRGAAVVSD
                     EPSVARAES"
     gene            2472..4043
                     /locus_tag="AC16JC_12030"
     CDS             2472..4043
                     /locus_tag="AC16JC_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5165468.1"
                     /translation="MSDAPSNDPTRISRRGWLQSLVAVPVLGVFVFNYLKKRAADDAR
                     KQAIMSELGVTESGPAIIPNAISKPPGSRIRLGIIGYGGEGESLVRHAGFASPKWLDE
                     SKQAVADDPRDKQLETYLRQDDLNVALTAVCDVFDVRAERAIAASQIAERPGGGGANP
                     PAKRYRRYTDLLDSKDVDAVIIATPDAWHSRITIDATAAGKHVYCEKCMTRTEEEAHA
                     VHAAVSGSKIVFQLGHQNRQSEPHNKAREVIAAGVLGPITLVEATTNRNTSWGAWVWD
                     IEKEGNPQTIDWDQFQEPAPHKVAFSPERFFRWRCWYDYGTGLAGDLLSHEYDAVNQI
                     LALGIPKSCSASGGIYYFKDGRDVPDTFQAVYEYPDRDLTFVYSSTLANGRDRGLAFM
                     GHDASMVVGSGLTVQADGDSTRYEQKIKDGVIDPGQPLFTYRPGFKGIDAVTSATERY
                     FASRGLLYTYRGGRRVSAYHLLIAEWLDVIRNGGRTSCNIDRGFEEAISCHMATRSYL
                     EGRKVTWDPVRRRIV"
     gene            complement(4211..5140)
                     /locus_tag="AC16JC_12035"
     CDS             complement(4211..5140)
                     /locus_tag="AC16JC_12035"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5165469.1"
                     /translation="MKPPRELPAHLALIAAQVSFGLFPVFGTLIFRPGGLTPLGVAVW
                     RLGAGAVLLLVLAVAVHGRRAIPARSDLARFVVVGLLGVGLNQAFFLEGLARSTPINA
                     TLVMCLIPVFTFGIAATVRQEPFSARRLAGLLIALVGLLPLLFVHGLHLGRYGLGNLL
                     MTGNALCYSAYLVVAKPLAPRYPPLVVIAWAYVLSLPLVPYFAWGEPLLPATGNAGAW
                     WALAYIVAFPTVIAYLLNMFALARVRASTTAVYVYAQPLVAVFASRLAFGETMSGTML
                     LAAATLFVGVWLVARPLQVNYSVPSPAPDASSS"
     gene            5265..5675
                     /locus_tag="AC16JC_12040"
     CDS             5265..5675
                     /locus_tag="AC16JC_12040"
                     /inference="COORDINATES: protein motif:HMM:NF039485.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HIR5165470.1"
                     /translation="MKETRARSGAAAAGGTATAVAPDDLVGHVLELINSPAVGGMAGL
                     VKRFQERGLGDVVSGWVGTGPNPPISPAQVETVLGSQQLGVLAGRTGLSLPELGSKLA
                     TLLPLIIDHLTPDGNVPSVAGLFTTTLDWLKEKP"
     gene            5691..6116
                     /locus_tag="AC16JC_12045"
     CDS             5691..6116
                     /locus_tag="AC16JC_12045"
                     /inference="COORDINATES: protein motif:HMM:NF039485.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HIR5165471.1"
                     /translation="MGLVDELTAKVPGAGAGTATAVAAEDPSKLIAAILAMIDEPKTG
                     GFAGLVQGFQDKGLGSIIAGWISPGHNPPITPAQVQTALGTERLQQLTRWSGEPLESL
                     PEQLAAILPIVVDHLTPDGQVPPSGTPLNQAMDWLDARG"
     gene            complement(6140..10072)
                     /locus_tag="AC16JC_12050"
     CDS             complement(6140..10072)
                     /locus_tag="AC16JC_12050"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR5165472.1"
                     /translation="MTWPVLVALTLRLSHVALLAVVGLGARPDSIPLPEHPRPDFQRD
                     LWLNLNGRWRFAFDPADSGERAGWPAAGVPGAREIVVPFSWGSPLSGVPDSGGGDIGW
                     YERTLTVPQMWTGHRVFIVFGASDWRTTAWLDGTKLGEYQGGYTPFSFELTPHLRPGR
                     AQRLVVRVDDAPHPFKLEGKQGYGKARGLWQTVYVEARGTAPLASVHFTPRRDLAGVG
                     VDVRLLEPAPRGLTLAVTFTNRDGRPTVTQRIAAGARTAHLDVPLPGARRWSLDDPFL
                     HEVVVSVAGPGGGFFPDSVRTYFGMRTIGVADVPGTAIPYVAINGTPVYLQLTLDQAY
                     HPEGYYTFPTDSALREEVLRPRRIGLNGLREHVKIETPRKLYWADRLGVLIMADVPNW
                     WGPPDSAAFREHEVALRGMIERDYNHPAVFAWVLFNETWGLETRVGNESRYLPEAQAR
                     VVRDYRLARSLDSTRLVEDNSPCCGRGHTETDLNSWHAYLPGWEWEAQVSRISDSTFP
                     GSTWNFERGWRQARQPMLNSEFGNVWGYEGSTGDVDWSWDYRRAIDAFRRHPKLAGWL
                     YTEHHDVINEWNGYWRADRSWKETGLGGIVDGMSLRDLHAPLYLAVGDPALARAARPG
                     ERVEVPLYASFLSGSTAFGDSLTIRAQLSGWNTLGEERTWSTYEMRIPYRPWMSLALA
                     PLSVTLPREPALAVLAVRLTDGSGTVLQRSFTAFVVQGDPPDSGVLADGSPVRVSRVA
                     AGAVADARWSLKHWTVLDGRKVDGAGSGYFEYHIPWPAGLDVGDVAGATFLVEASAKR
                     LNGKDRDSTVAAGGDYMRGGGFHDRSANRNSYPMTSAAPYPSAVTVRVNGTLAGAVEL
                     ADDPADSRGILSWYAQPRDGHLYEAGSYGQLVRVAIPREALVAAARTGQVVVRLEVGE
                     ALPGGLAIYGAQFGRYPVDPTVLFVLRGRGGTAAGAQPRVTRAPFGRLPDGRAVEQFT
                     LTNLHGVEVRAITYGAIITAVRTPDRDGKLDDVALGFDSLGPYLRQSPYFGAIVGRYA
                     NRIAQGRFQLDGRTYRLAQNNGPNSLHGGLRGFDKVLWTGEPFEGDSGAGVVFRYTSP
                     DGEEGYPGTLQVRVTYRLTPADELVVEYEAQTERATPVNLSQHTYWNLHGAGRGTILD
                     HVLTLDASAFTPVDSTLIPTGAITPVAGTPFDFRTPTAIGARIAESDVQLRFGGGYDH
                     NWVLDRSGGPADTGLVHAARLVDPASGRALDVSTTEPGIQFYSGNFLDGTLTGKDGHV
                     YVHRGGLCLETQHFPDSPNHANFPSTILRARAVYHSRTVFRFGVVRQP"
     gene            complement(10115..10957)
                     /locus_tag="AC16JC_12055"
     CDS             complement(10115..10957)
                     /locus_tag="AC16JC_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020706661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5165473.1"
                     /translation="MLVASTVGASAWAQARGAYAAVNGLRIYYEIHGPAADTNAPLVL
                     LHGGVGGIEMFGPNLPALAAGRRVIAVDLQGHGRTADVDRPLRCESMADDIAALLGVL
                     RIARADILGYSLGGCVALQTAIRHPDVVRKLVIVSAGFRRSGFYPEVLQAMDHFGPTA
                     AAGMKQSPLGTLYPDVDWARLFTKLGDLLRQDYDWSKDVAAIKAPTMLVFADADAFQP
                     AHIMEFYGLLGGGRRDAGLDGSGRPVAWLAVIPGATHYSILSTTMVAELVTPFLDQPP
                     PNAR"
     gene            complement(11010..11912)
                     /locus_tag="AC16JC_12060"
     CDS             complement(11010..11912)
                     /locus_tag="AC16JC_12060"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5165474.1"
                     /translation="MTSRREFLQTSAAILAASAWPRGGGERPPLGFSTLGCPRWDWLQ
                     VLDFAAAHGYGAVELRGLQETMDLTQRPEFAPGRMSATKAQLAHHGLVVSCLGSSANM
                     HEMDPATRAAQLDEARRFIDLARALGAPYVRVFGNQYLPGVPRDEMLDHIARSLSELG
                     RYARERGVTVLIESHGDFTDSPSLRAILERADSGAVALLWDAHHTFVSGKEEPEETVR
                     QLGRWIRHTHLKDSVASGNDRRYVLTGTGDVPVRRQVEALMKSGYRGYYSFEWEKRWH
                     PEIEEPEVALAQFVTVVSEYLRGR"
     gene            11977..12747
                     /locus_tag="AC16JC_12065"
     CDS             11977..12747
                     /locus_tag="AC16JC_12065"
                     /inference="COORDINATES: protein motif:HMM:NF018178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-disaccharide hydrolase"
                     /protein_id="HIR5165475.1"
                     /translation="MIARCFVFFLVAAGAGPIAAQTSPAGRPNELSAGERAAGWRLLF
                     DGRTLRGWRGLGSDSVPTAHWVVVDGAIKKVASGQVPTLPDGQPAQGGDLMTVDSFQD
                     FELSFEWRVTPGANSGVKYNVSEDLSVREAPNHAALGFEYQVLDDDRHPDGKLPTHRA
                     GALYDLVAPNDRKRLHPVGEWNRSLVIFRGRHGEHWLNGERIVAFELGSAGFDSLLAA
                     SKFRTLPWFGERRAGGGHIVLQDHGDEVYFRSIKVRPL"
     gene            12744..14060
                     /locus_tag="AC16JC_12070"
     CDS             12744..14060
                     /locus_tag="AC16JC_12070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020715418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5165476.1"
                     /translation="MNRRKFLKTTSAAGLGMVASRVGPPWVGAASPGERIVVAVMGLN
                     GRGTVLGKAFARAPNATVGYVCDVDSRVLARAAADVASLQTRPPKAIGDFRRALDDKT
                     VDALVVAAPDHWHAPATILALQAGKHVYLEKPCGHNAREGELLVQAQRKYERVVQMGT
                     QQRSAPRSIEVIQAIREGLIGRPYLARGWYANTRGTIGRGKPAPVPATLDYELWQGPA
                     PRTPYRDNVIHYNWHWFHRWGTGEIANNGTHELDICRWALGADTCTRVVSTGGRYYFD
                     DDWEFPDTQEAAFEFPGGKTIIWHGESCNGLQPFGRSRGTAVLGTGGSAVLDRDGYVV
                     YDPKGRVIREVVAAKPGDALNIGADDEATTLHIANFVDAIRTGAPLHQPIGEAVKSVL
                     LCHLGNIAQTTGRALRTDPATGRIVDDADAMKLWEREYAPGWVPAL"
     gene            complement(14274..15311)
                     /locus_tag="AC16JC_12075"
     CDS             complement(14274..15311)
                     /locus_tag="AC16JC_12075"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004668 - protein-arginine deiminase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase family protein"
                     /protein_id="HIR5165477.1"
                     /translation="MPAEWERHAATWLGWSHNRTDWPGKFAPIPWVYGEIVRHLAPGE
                     TVRILVDSPARERSATSVLRRAGVAVPGDAVQFLRFPTNRGWTRDSGPIFVRRDAPRS
                     EIAIAHFHFNAWAKYRDWAKDARLPELAARRLGVPLFDVRRGERRVVLEGGSIDVNGA
                     GTVLTTEECLLDPVVQTRNPGFTRADVEAVLHDALGATNVVWLGRGIAGDDTHGHVDD
                     VCRFVGPRTVVLCREQNPADPNYAPLEENRERLQDARAEDGARLEVLELPMPAPLTFD
                     GQRLPASYANFYIANAAVLVPTFNDPKDRIALGLLAEVFPDRPVVGIHAVDLVWGLGT
                     LHCLTQQEPAA"
     gene            complement(15347..16258)
                     /locus_tag="AC16JC_12080"
     CDS             complement(15347..16258)
                     /locus_tag="AC16JC_12080"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase"
                     /protein_id="HIR5165478.1"
                     /translation="MTKQAGTARHTTVGLVQMRMTADPQANLERAITRVREAAARGAT
                     LVCLPELFRTLYIGQREDHQLFDLAEPVPGPSTEALGAVARELGIVIVASLFERRAPG
                     LYHNTAAVLDADGRVAGIYRKMHIPDDPSYYEKFYFAPGDLGFRAFDTAAGRIGTLVC
                     WDQWYPEGARLTALQGASILVYPTAIGWHPAEKAQVGAQQLDAWRTIQRSHAIANGCY
                     VAAVNRVGLERLDGQGPGLEFWGHSFLADPFGVVVAEAPADAETILVAPLDPVRLEEV
                     RRNWPFLRDRRVDAYGGLDRRYLGDPS"
     gene            complement(16343..17395)
                     /locus_tag="AC16JC_12085"
     CDS             complement(16343..17395)
                     /locus_tag="AC16JC_12085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR5165479.1"
                     /translation="MISRPLVVPTLLMVTALAWPGTSPAQTFKVAKYNIGGDGGTDYL
                     TAEPGTGRVFISRSTHVMVVDGSTGKVLGDIPDTPRVHGIALVPRSHHGFTTNGGDST
                     VTMFDLRTLAVIRKIPVPTGGLDGIMYDAFSDRIILTNHSHPVGTAVALDAGSGAIVG
                     TAELESTSPEGAASDGKGTLFVNNEGTSTIQVIDVKTMTVRASWPLAPCQGPTGIAYD
                     AKTHRIFSGCRRTSVVVDPASGKVVATIANGEGVDALGWDPAEKLIYIPAGRDSSVTI
                     AHEDSPDTYRVVATVPTMRGAKTISVDPVRHIAYLFQPEFGPPPPPAPDAPAPQPGFG
                     GLRGPVIAAWFFAISH"
     gene            complement(17502..19223)
                     /locus_tag="AC16JC_12090"
     CDS             complement(17502..19223)
                     /locus_tag="AC16JC_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165480.1"
                     /translation="MEDDLIANAGVVMSARATASVNTIARGRLLVGSGLPLALAVVSF
                     AACKDDMTATRTPNPQSVSVLTQHNDNTRAGWNDHETALTPSNVNLRHFGKVFTLAVD
                     DQVYAQPLVVGRVPVNGAERNVVYIATVNNTLYAFDGDDGTLYWARNYSAPGMRPPRN
                     TDMTGACNGSYRDFSGNIGMVGTPVIDAARRTLYVVARSTDGNAFVQHLHAVSLVDGS
                     EATGSPVRITATYPGSGDGSVANVITFDGQRQNQRQGLTLLNGVVYVTFASHCDWGPY
                     HGWILGYDAVTLQPRVVYNATPNGYAGGLWESGMGMAADAAGSLYVVAGNGTVGDSGD
                     PTSLTNRGESALKLVPSGSTLQVASYFTPFDYQYLNDYDVDYGSVGALLIPNSSYYLT
                     GGKDGYLYLLNTNDMGGYQAAANQVQQVVPLTVSEMHCQPAYYRGSAREFVYVWPENN
                     PLRAIPFDRGSNRLDRLAELAFGSGGPTGHSGAMLSVSSDSSRDGTAILWASYAVSGD
                     AEHDVSPGILRAFDANDVTRELWNNHQDLARDGAGTYAKFASPTIANGHVYLPTFSNQ
                     VVVYGLR"
BASE COUNT         3069 a         6671 c         6762 g         2851 t
ORIGIN      
        1 tcctgcactg tgacatcacg ctccaggcgg cgcaggccgg caagcatgtc gtgatcgaga
       61 agccgttgtg catgaatctg gccgaagcgg atcgcatgat cgacgggtgc agggcggcgc
      121 aggtcaaact catgtatgcc gaagagctgt gtttcgcccc gaagtacgtg aggctgaagg
      181 ggctgctgga cgagggcgcg ctcggcaagc cggtcctgct caaacaatcc gagaagcacg
      241 acgggccgca cgcggagcat ttctgggacg tggagcgatc gggtggcggg gtaaccatgg
      301 acatgggctg ccacgccatc gagttcttcc gctggctgaa cggcaatacc cccctcaggt
      361 ccgtctatgc gcagatgagc accagcgtcc atacggcgaa gacgaaaggc gacgacaatg
      421 cgatcatcat cctcgaattc gagaacggcg tgatcgcggt cgccgaggag agctggacca
      481 agctcggcgg catggacgac caggcagaga tccacggctc ggagggagtc gcctatgccg
      541 acctgctgca cggcaatgcg atcgagacct acagcacgcg cggggtcggg tacgctgtcg
      601 aaaaggcagg gatcaccagg gggtggagct tcacgatgta cgaggaggtc tggaattacg
      661 ggtttcccca ggaactggag cacttcgtgc attgcgtgaa gcacgacgag acgccccggg
      721 tgacgggtga agatggcaga gcggtgctcg agacgatatt cgcggcgtac gaatcggcag
      781 gtacgggcag gaaggtgacg atgccgttcg tgagcgatgc cgacaggccg tacaagctct
      841 ggaggaagtg attcgcttct tgcagtcttt ttcacgaagc ctttaaagac cctgccgtag
      901 gctggctcac cgttccgtgc aggagccagt gactccgcag acccccgacc tgccggcgag
      961 ccgcatcggc gcgctcacca gcttgggctt cgagcaggtc cagccgtccg tgtgcgagac
     1021 agcggtgcac gacgccggcc acgacggctt ccgcgtcctg tcccggcgtg ccgcgatgag
     1081 cacgctcgtc gacatcgtcg cgatccacca gtccgccggc cttgccgagg aagcgatcgg
     1141 ccgggccttc gaggagatgg accggctcgt cgcgatcttc aaccgccacg accgggcctc
     1201 cgcgctgtcc ctgctcaaca gcgagggaca cctcgaagac ccgccgcccg agctggcgct
     1261 ggtcctttcc gatgcgctga actgccatcg ggtgagcgac ggggccttcg acccgacggt
     1321 ggccccactc gtggatctgt tccaggcctg cttcaccggt cccactccac atgagccgac
     1381 ggccgccgaa atcgcggagg cgcgcggcct ggtgagcgcc ggcgcgatca cggcgtcgcc
     1441 acgccgcgta cggctgacgc ggcgcggcac gaccctcacg ctggacggca tcgcgaaagg
     1501 attcatcgtg gaccggatgg cggcgacgct cgagggcgcc ggcgtccatc gctacctggt
     1561 ggaagcgggc ggcgacatcc gcaccggcgg caggaaggag gaccggcttc cctgggccgt
     1621 ggccatccgc gacccggaga gcggggggcg cggccgcctc cccgggacga tccagctttc
     1681 gggcgccgcc gtcgccacct cggggtccta cgagcgctac tacgatccgg agcgccgctt
     1741 ccaccacatc atcgacgcgg cgcgtggctc ctcgccgcac gactgcgtca gcgccaccgt
     1801 gatcgccccg accgcgacgg cggccgacgc gctcgccacc gccgtcctgg tgctgggatc
     1861 gcgggccggc aaggcgctcg tcgagcgtct ccccggttgc gcctgtcaca tcgtccgctc
     1921 cgacggacgc gtcacccact cgcatggctg gaaggagtcg ccggcatgac ccgtccccaa
     1981 tccgccaccc tcgtcaccct gcgcctgctc gtcggatggc actttctcta cgaggggctg
     2041 gccaagctgg cgaaccccta ctggacctcc gccgagtacc tcgaccaggc cgggtggctg
     2101 ttcaagaagc tgttcgtcgc gctggcggcg agcccgagcg cggtcacggt ggtggacttc
     2161 ctgaacgagt ggggcctcgt cctcatcgga ctcggcctgc tcgtcgggtt gttcacccgc
     2221 acggccacca tcgccgggat cgtgctgctg ctgctgtact acatcgccgc gccgccgctc
     2281 gtgggcatca gctccgcggt gccgcgggag ggcagctacc tgctcgtgaa caaggtgctg
     2341 atcgaggcgg ccgcgctggc cgtgctcctg gcgttcccta ccggccgaag cttcggcctg
     2401 gacgggctga tcttccggcg gcgcggtgcc gccgtggtgt cggatgaacc atccgtcgcg
     2461 agggccgagt catgagcgac gcaccttcca acgatcccac ccgcatctcc cggcgcggct
     2521 ggctccagtc cctcgtcgcg gtgccggtgc tcggtgtctt cgtcttcaac tacctgaaga
     2581 agcgcgcggc cgacgacgcc cggaagcagg cgatcatgtc ggagctgggg gtcaccgaga
     2641 gcgggcccgc catcattccc aacgccatct cgaagccccc cgggagcagg atccggctgg
     2701 ggatcatcgg gtacggcggc gagggggagt cgctggtgcg gcacgcgggc ttcgcctcgc
     2761 ccaagtggct cgacgagtcg aagcaggcgg tggccgacga cccgcgcgac aagcagctcg
     2821 agacctatct ccgccaggac gacctgaacg tcgccctgac cgccgtgtgc gacgtgttcg
     2881 acgtgcgcgc cgagcgggcc attgcggcct cgcagatcgc cgagcgcccg ggaggcggcg
     2941 gcgccaaccc gccggccaaa cgctaccgcc gctacaccga tctgctcgac agcaaggacg
     3001 tggacgccgt catcatcgcg acgcccgatg cctggcattc ccgcatcacg atcgacgcca
     3061 ccgccgccgg gaagcacgtg tattgcgaga agtgcatgac ccggaccgag gaggaggccc
     3121 acgccgtgca cgctgcggtg agcgggtcga agatcgtgtt tcagctggga caccagaacc
     3181 gccagtccga gccccacaac aaggcgcgcg aggtcatcgc ggccggggtg ctcggcccga
     3241 tcaccctggt cgaggcgacc accaaccgca acacctcgtg gggcgcctgg gtgtgggata
     3301 tcgagaaaga ggggaatccg cagacgatcg actgggacca gttccaggag ccggccccgc
     3361 acaaggtggc gttcagcccc gagcgcttct tccggtggcg ctgctggtac gattacggca
     3421 ccggcctggc cggcgatctc ctgtcgcacg agtacgacgc ggtgaaccag atcctggcgc
     3481 tggggatccc caagtcgtgc agcgcatccg gagggatcta ctatttcaag gacggccgcg
     3541 acgtccccga caccttccag gcggtctacg agtacccgga tcgcgatctg accttcgtct
     3601 acagctccac gctggccaac ggtcgcgacc gcggcctcgc cttcatgggg cacgacgcct
     3661 cgatggtcgt ggggagcggt ctcaccgtgc aggccgacgg cgactccacc cggtacgagc
     3721 agaagatcaa ggacggcgtg atcgatcccg gccagccgct gttcacctac cggccggggt
     3781 tcaaggggat cgacgcggtg acgtcggcca cggagcggta cttcgcgagc cgggggctgt
     3841 tgtacaccta ccgcggcggg cgccgcgtgt cggcgtacca tctgctcatc gccgagtggc
     3901 tcgacgtgat ccgcaacggg ggccggacga gctgcaacat cgaccgcggc ttcgaggagg
     3961 cgatcagctg ccacatggcg acgcgctcct acctcgaggg ccgcaaggtg acgtgggatc
     4021 cggtacggcg gcggatcgtg tagggcgcct catcccctga ctcgctctcc aggggcctct
     4081 ccccctgtcc ccctctcggg ggacctctcc ccctgtcccc ctctccatgg gccgctcccc
     4141 ctgtccccct ctccgccatg cggagagggg gaactaagtg caagatctcg agacagccac
     4201 aaacggcgac tcacgaggat gatgcgtccg gagccgggga tgggactgag taattgacct
     4261 gcagcggccg ggccaccagc cagactccta caaatagggt cgctgccgca agcagcatcg
     4321 tgccgctcat cgtttccccg aacgccaatc gactcgcgaa gaccgcgacc agcggctgcg
     4381 cgtagacgta caccgccgtc gtcgaggccc gcacgcgcgc cagcgcgaac atgttgagca
     4441 ggtatgcgat gaccgtcggg aacgccacga tgtaggcgag cgcccaccac gcgccggcgt
     4501 tgcccgtcgc cgggaggagc ggctcacccc acgcgaagta cggcaccagc ggcagcgaca
     4561 gcacgtacgc ccaggcgatg acgacgagcg gcggatagcg cggcgccagc ggcttcgcca
     4621 cgaccaggta ggccgagtaa cagagcgcgt tcccggtcat cagcagattg cccagtccgt
     4681 agcggcccag atggaggccg tgcacgaaca acagcggaag cagccccacg agcgcgatca
     4741 gcaaccccgc gaggcgccgg gcgctgaacg gctcctgccg caccgtcgcg gcgatcccga
     4801 aggtgaagac ggggatgagg cacatgacga gggtggcgtt gatgggcgtc gaccgcgcca
     4861 gtccttcgag aaagaacgcc tgattgagcc ccacgcccag caacccgacc accacgaatc
     4921 gcgcgaggtc ggagcgggcg gggatggccc ggcggccgtg aaccgccacc gcgagcacca
     4981 gcagcaacac tgccccggcg ccgagtcgcc acacggcgac gcccagcggg gtgaggccgc
     5041 cgggacggaa gatcagggtg ccgaagacgg gaaacagacc gaaggagacc tgcgccgcga
     5101 tcagggcgag atgggcaggg agctcccggg ggggcttcac gtgcgcgaag ataacgaggg
     5161 gtgaggcttc acggccgctt catggtcccc accctagttg ttcaccagat gtgctatagt
     5221 cgaagacggt cgcggcgctc gccgcgcatc accaaggagt cggtatgaag gagacgagag
     5281 cacgcagcgg cgccgccgca gccggcggga ccgcgaccgc cgtggccccc gatgacctgg
     5341 tcggccacgt gctggagctg atcaacagcc cggcggtggg cggcatggcc ggactggtga
     5401 agcgcttcca ggagcggggg ctgggagatg tcgtgtccgg atgggtcggc accggcccca
     5461 acccgccgat ctcacccgcc caggtggaga cggtcctggg cagtcagcag ctcggggtgc
     5521 tcgccggcag gaccggcctg tcgctccccg agctcgggag caagctcgcg accctcctgc
     5581 cgctcatcat cgatcacctg acccctgacg ggaatgtgcc cagcgtcgcc ggcctgttca
     5641 cgacgacact ggattggctc aaggagaagc cgtaaaggga ggagccacca atgggactcg
     5701 tggacgagct aaccgccaag gtccccggtg caggcgccgg gacggccact gccgtggccg
     5761 cggaggaccc ctcgaagctg atcgccgcga tcctcgcgat gatcgacgag ccgaagaccg
     5821 gcggtttcgc cggactggtg cagggattcc aggacaaggg gttgggaagc atcatcgcgg
     5881 gatggatcag ccccgggcac aaccccccga tcacgcccgc ccaggtgcag accgccctgg
     5941 ggaccgagcg gctgcagcag ctcacgcggt ggagcggcga gccgctcgag tcactcccgg
     6001 aacagctcgc ggcgatcctg ccgatcgtcg tcgatcacct gacgcccgac gggcaggtgc
     6061 cgccgagcgg cacgccgctc aatcaggcga tggactggct ggatgcgagg ggatagcgga
     6121 ggccggtgac gcgagccact cagggctgcc ggacgacgcc gaaccggaac acggtgcgcg
     6181 agtggtacac cgcccgggca cgcaggatgg tcgaggggaa gttggcgtgg ttcggggaat
     6241 cggggaaatg ctgcgtctcg aggcacagcc cgccgcgatg cacgtacacg tgcccgtcct
     6301 ttcccgttag ggtcccgtcc aggaagttac ccgagtagaa ctggatcccc ggctccgtgg
     6361 tgctgacgtc gagcgcgcgg ccgctcgcgg ggtccacgag gcgcgccgcg tgcaccagcc
     6421 ccgtgtccgc tgggcctccg ctccggtcca gaacccagtt gtggtcgtag cccccgccga
     6481 agcgcagctg gacgtcggac tcggcgatgc gcgcgccaat cgccgtcggc gtgcggaagt
     6541 cgaacggcgt gccggcgacc ggcgtgatcg cgccggtggg gatgagcgtg gagtcgacgg
     6601 gcgtgaaggc cgatgcatcg agcgtgagga cgtgatcgag gatcgtgccg cggccggcgc
     6661 cgtgcaggtt ccagtaggta tgctgggaca ggttgacggg cgtcgcgcgc tcggtctgcg
     6721 cctcgtactc caccaccagc tcgtccgccg gcgtgagccg gtaggtcacg cggacctgca
     6781 gcgttcccgg gtatccctcc tcgccgtccg ggctggtgta gcggaacacg acccccgcgc
     6841 cgctgtctcc ctcgaacggc tcaccggtcc acagcacctt gtcgaagccg cgcagcccgc
     6901 cgtgcaggct gttgggaccg ttgttttgcg ccagccggta ggtccgcccg tcgagctgga
     6961 accgtccctg cgcgatacgg ttggcgtagc ggcccacgat cgccccgaag tagggggact
     7021 gccgcaggta gggcccgagg ctgtcgaatc ccagggcgac gtcgtcgagc ttgccgtccc
     7081 ggtccggagt ccggaccgcg gtgatgatgg cgccgtaggt gatcgcccgg acctcgacgc
     7141 cgtggagatt ggtgagcgtg aactgctcca cggctcggcc gtcgggcagc cgcccgaatg
     7201 gcgcgcgggt cacgcgcggc tgcgcgccgg ccgccgtacc gccgcgaccc cgcagcacga
     7261 acagcaccgt cggatcgacc ggatagcgac cgaactgcgc cccatagatc gcgagccctc
     7321 ccggtagcgc ctcccccacc tcgaggcgga ccaccacctg ccccgttcgc gccgcggcga
     7381 cgagcgcctc gcgcgggatc gcgacgcgga ccagctggcc gtacgagccg gcttcgtaca
     7441 aatgcccgtc gcgcggttgg gcgtaccagc tcaggatgcc gcgcgagtcc gccggatcgt
     7501 cggcgagctc gacggcgccg gcgagcgtgc cgttcacccg tacggtgacc gcgctcgggt
     7561 acggcgcggc actcgtcatc gggtagctgt tccggttcgc gctgcggtcg tggaagccgc
     7621 cgccccgcat gtaatcgccg ccggccgcga cggtcgagtc gcggtccttg ccgttcaggc
     7681 gcttcgccga cgcctcgacc aggaacgtcg cgccggcgac gtcgccgacg tcgagccccg
     7741 cgggccaggg aatatggtat tcgaagtagc cgctccccgc cccgtccact ttgcggccat
     7801 cgagcacggt ccagtgtttg agcgaccagc gggcgtcggc caccgcgccg gccgcgacgc
     7861 gagacacgcg cacggggctg ccgtccgcca ggacgcccga gtcggggggg tcaccctgca
     7921 ccacgaacgc ggtgaagctc cgctgcagca ccgtcccgga cccgtccgtc agtcgcaccg
     7981 ccagcacggc cagcgccggc tcccgtggca gcgtgacgga caacggcgcg agcgccaggg
     8041 acatccaggg ccggtagggg atgcgcatct cgtacgtgct ccaggttcgt tcctcgccca
     8101 gcgtgttcca tccggacagc tgcgcgcgga tcgtgagcga atcgccgaac gcggtgctgc
     8161 cggagaggaa cgacgcgtac agcgggactt ccacccgctc gcccggccgc gccgcgcggg
     8221 ccagcgccgg gtcgcccacc gcgaggtaca gcggcgcgtg caggtcccgc agcgacatgc
     8281 cgtccacgat cccgcccagc cccgtctctt tccacgaccg gtcggcgcgc cagtacccgt
     8341 tccattcgtt gatgacgtca tggtgctcgg tatagagcca gccggcgagc ttggggtggc
     8401 ggcggaaggc gtcgatcgcg cggcggtagt cccagctcca gtccacgtct ccggtgctgc
     8461 cttcgtagcc ccacacgttg ccgaattcgg agttgagcat cggctggcgc gcctggcgcc
     8521 agccgcgctc gaagttccag gtcgagcccg ggaaggtgct gtcgctgatg cggctcacct
     8581 gggcctccca ttcccagccc gggaggtagg cgtgccacga attcaggtcg gtctcggtgt
     8641 gcccgcggcc gcagcagggc gagttgtctt cgacgaggcg ggtcgagtcg agggagcggg
     8701 cgaggcggta gtcgcgcacc acgcgcgcct gggcttcggg cagataacgc gactcgttcc
     8761 cgacgcgcgt ctcgagaccc caggtctcgt tgaacagcac ccacgcgaac accgcggggt
     8821 gattgtagtc gcgctcgatc atgccgcgca gcgcgacctc gtgctcgcgg aacgcggcgg
     8881 agtcgggcgg tccccaccag ttggggacgt cggccatgat gagcacgccg agccggtcgg
     8941 cccagtacag cttgcgcggc gtttcgatct tgacgtgctc gcgcaggccg ttgaggccga
     9001 tgcggcgcgg ccgcagcact tcctcgcgca gcgcgctgtc cgtggggaag gtgtagtagc
     9061 cctccgggtg atacgcctgg tcgagggtga gctggaggta gaccggcgtg ccgttgatgg
     9121 cgacgtaggg gatcgccgtc cccgggacgt cggccacgcc gatggtgcgc atcccgaagt
     9181 aggtccgcac gctgtccggg aagaagccac ctcccggtcc cgccacactc accacgacct
     9241 cgtgcagaaa cggatcgtcg agcgaccagc gacgcgcgcc ggggagcggg acgtccaggt
     9301 gcgccgtgcg ggcgccggcc gcgatgcgtt gcgtgaccgt gggacgcccg tcgcgattgg
     9361 tgaacgtcac ggcgagcgtc aacccgcgcg gcgcgggctc gagcaggcgc acgtccaccc
     9421 cgaccccggc gaggtcgcgc cgcggcgtga agtgcaccga cgcgagcggc gcggtgccgc
     9481 gcgcttcgac gtacaccgtc tgccacaggc cgcgcgcctt tccgtagccc tgtttgccct
     9541 cgagcttgaa cgggtgcggc gcatcgtcca cgcggaccac caggcgctgc gcccggccgg
     9601 gccgcaggtg gggggtcagc tcgaacgaaa acggcgtata cccgccctgg tattcgccga
     9661 gcttcgtgcc gtcgagccac gcggtggtgc gccagtccga cgcgccgaag acgatgaaga
     9721 cgcggtggcc ggtccacatc tggggcacgg tgagcgtgcg ctcgtaccag ccgatgtcgc
     9781 ccccgcccga gtccggaacg cccgagagcg gcgaccccca ggagaacggc accacgatct
     9841 cgcgcgcgcc ggggaccccc gcggcgggcc agccggcgcg ctcgcccgag tccgccgggt
     9901 cgaacgcgaa gcgccagcgg ccgttgaggt tcagccagag gtcgcgctga aaatcggggc
     9961 gcgggtgctc ggggagcggg atggagtcag gccgcgcgcc aagccctact acggccagga
    10021 gcgccacatg cgagagccgc agcgtcagcg ccacgagcac cggccacgtc atgatcgatg
    10081 gcatggtcga gcggccggcc gtgccgcgcg cccgtcacct cgcattgggc ggcggctggt
    10141 cgaggaacgg tgtgaccagt tcggcgacca tcgtggtcga cagaatgctg taatgggtgg
    10201 ccccggggat gacggcgagc caggcgaccg ggcggccgga gccgtccagg ccggcatcgc
    10261 gtcgtccccc gccgagcagg ccgtagaact ccatgatgtg cgccggctgg aatgcatcgg
    10321 cgtcggcgaa gacgagcatc gtcggcgcct tgattgccgc gacgtccttt gaccaatcgt
    10381 agtcctggcg gagcagatca cccagcttgg tgaacagccg cgcccagtcg acatcggggt
    10441 agagggtgcc gaggggcgat tgcttcatgc cggccgccgc cgtcggccca aagtgatcca
    10501 tggcttgcag gacctcgggg tagaacccgc tgcgcctgaa cccggccgag acgatgacga
    10561 gctttcgcac gacgtccggg tgccggatcg ccgtttgcag cgcgacgcag ccgcccagcg
    10621 agtaccccag gatgtccgcc cgggcgatcc gcagcacccc gaggagggcg gcgatgtcgt
    10681 cggccatcga ttcgcagcgc agcgggcgat cgacgtcggc cgtgcgaccg tggccctgca
    10741 ggtccacggc gatgacgcgg cgcccggccg ccagcgccgg caggttcggg ccgaacattt
    10801 cgatgccgcc caccccgccg tgaaggagaa cgagaggcgc gttcgtgtcc gcggcgggtc
    10861 catggatctc gtagtagatc cgcaggccgt tcacggcggc atacgccccc cgtgcctgcg
    10921 cccacgcact agcgcccacg gtggaggcga ccagcatcag ggagaccaag accggtccca
    10981 tatcgagcat actctccgtc cctcctccgt cagcgcccgc gcaggtactc gctcaccacg
    11041 gtgacgaact gcgccagcgc gacctccggc tcctcgatct ccgggtgcca gcgcttctcc
    11101 cactcgaagc tgtaataacc gcggtagccg ctcttcataa gtgcctcgac ctggcgccgc
    11161 accggcacgt cgcccgtccc cgtgagcacg tagcggcggt cgttgccgga ggcgaccgaa
    11221 tccttgagat gggtgtgccg gatccagcgt ccgagctgcc gcaccgtctc ctccggctcc
    11281 tcctttcccg acacgaaggt gtgatgcgcg tcccagagga gcgccaccgc gcccgagtcg
    11341 gcgcgctcca gaatcgcccg cagcgaggga gaatcggtga agtcgccgtg cgattcgatg
    11401 agcaccgtga ccccccgctc ccgcgcatac cggccgagct cgctcaggct ccgcgcgatg
    11461 tggtcgagca tctcgtcgcg cggcacgccg gggaggtact ggttgccgaa cacccgcacg
    11521 tagggcgcgc ccagggcccg cgccagatcg atgaagcggc gcgcctcgtc gagctgggcg
    11581 gcccgtgtgg ccggatccat ctcgtgcatg ttggcggagc tgccgaggca ggagacgacg
    11641 agtccatggt gggcgagctg cgccttcgtt gcggacattc gcccgggcgc gaactcgggg
    11701 cgctgggtga gatccatcgt ctcctgcagg ccgcgcagct cgacggcgcc gtagccgtgg
    11761 gcggccgcga agtcgagcac ctggagccag tcccagcgcg ggcagccgag agtcgagaag
    11821 cccaggggcg gccgctcccc gcctccccga ggccaggcgg acgccgcaag aatcgcggcc
    11881 gacgtctgga gaaactcgcg acgactcgtc accgaccgtg ccctcctccc ttgcgccgca
    11941 ttgacaccct gcgcaacaat atggtactac tgggaaatga tcgcccgatg ttttgtcttc
    12001 ttcctggtcg cagccggtgc ggggccgatc gcggcccaga cctcgccggc cgggcgaccc
    12061 aacgagctga gcgccggcga gcgtgctgcc ggctggcggc tgctgttcga cggccggacg
    12121 ctgcgcggct ggcgcggcct cggctccgac agcgtcccca cggcccactg ggtcgtggtg
    12181 gacggcgcca tcaagaaggt ggcgagcggc caggtgccca cgctgcccga cggccagccc
    12241 gcgcagggcg gcgacctcat gaccgtggac tcgttccagg acttcgagct gtcgttcgag
    12301 tggcgcgtca cccccggcgc caacagcgga gtgaagtaca acgtctccga ggatctgtcg
    12361 gtacgggaag ctcccaacca cgcggccctc ggcttcgagt accaggtcct ggacgacgac
    12421 cggcaccccg acggcaagct ccccacgcac cgggcgggcg cgctgtacga tctcgtcgcc
    12481 cccaacgacc gcaagcgcct gcacccggtg ggcgagtgga accggtcgct ggtgatcttc
    12541 cgcgggcggc acggcgagca ctggttgaac ggcgagcgca tcgtggcgtt cgagctcggc
    12601 agtgccggat tcgactcgct gctggcagcc agcaagttcc ggacgctgcc gtggttcggc
    12661 gagcggcgcg ccggcggcgg gcacatcgtg ctccaggacc acggcgacga agtgtacttt
    12721 cgcagcatca aagtgaggcc gctatgaacc gccgcaagtt cctgaagacc acctcggccg
    12781 ccggactggg catggtcgcg tcgcgggtcg gacccccctg ggtcggcgcc gcctcgccgg
    12841 gcgagagaat cgtcgtcgcg gtcatgggcc tgaacgggcg cggcacggtg ctgggcaagg
    12901 ccttcgcgcg ggcgccgaac gccaccgtcg gctacgtgtg cgacgtggac tcgcgggtgc
    12961 tggcccgcgc cgcggcggac gtcgccagcc tgcagactcg accgccgaag gcgatcggag
    13021 acttccggcg ggcgctggac gacaaaaccg tggacgcgct cgtggtcgcc gctccggatc
    13081 actggcacgc gccggccacg atcctcgccc tgcaggccgg gaagcacgtg tatctcgaga
    13141 agccctgcgg ccacaacgcg cgcgagggcg agctgctggt gcaggcgcaa cgcaagtacg
    13201 agcgcgtggt ccagatgggt acgcagcagc gctcggcgcc gcgctccatc gaggtcatcc
    13261 aggcgatccg cgaaggcctg atcgggcgcc cgtatctcgc ccgcggctgg tacgcgaaca
    13321 cccgcggcac gatcggccgc ggcaagccgg cgcccgtgcc ggcgacgctc gactacgagc
    13381 tgtggcaggg ccccgctccg cgcacgccgt atcgcgacaa cgtgattcac tacaattggc
    13441 actggttcca ccgctggggg accggcgaga tcgccaacaa cggcacgcac gagctggaca
    13501 tctgccgctg ggcgctcggc gccgatacgt gtacgcgggt cgtgtcgacg gggggccggt
    13561 actatttcga tgacgactgg gagtttcccg acactcaaga ggcggcgttc gagttcccgg
    13621 gtggcaaaac catcatttgg cacggagaga gctgcaacgg gctgcagccc ttcggccgct
    13681 cgcgcggcac cgcggtgctc ggcacgggag ggagcgccgt gctcgatcgc gacggctacg
    13741 tcgtctacga tccgaagggc cgcgtgatcc gggaggtggt cgccgcgaaa ccgggcgatg
    13801 ccctgaacat cggcgccgac gatgaggcga cgacgctcca catcgccaac ttcgtggacg
    13861 cgatccggac cggcgccccg ctacaccagc ccatcgggga ggccgtgaag agcgtcctgc
    13921 tgtgtcacct ggggaacatc gcgcagacta ccggtcgggc actgcgcacc gaccctgcca
    13981 ccggtcgcat cgtcgatgat gcagacgcca tgaagctgtg ggagcgggag tacgccccgg
    14041 gctgggtgcc ggccctttag gcaggctctg cggaagtgac gcgacgccgg gggtgggggc
    14101 ggccggacgc gttggacgcg ggggcgctcg acccggacca gcgtttcgca tcccattgcc
    14161 ggccgcgagc gacaatgacc gggccgtgcg gcccccgcgt cctcgcgtct gggcgccccc
    14221 acccccggcg tcgcgcggac ttgccctgat cgcaacaatg ctggagggga ctctcaggcc
    14281 gccggttcct gctgggtgag gcagtgcagc gtgccgagcc cccacacgag atccaccgca
    14341 tggatgccga cgaccggccg atcggggaac acctcggcca gcagccccag ggcgatccgg
    14401 tccttcggat cgttgaacgt cggcaccagg accgccgcgt tggcgatgta gaagttggcg
    14461 tagctcgcgg ggagccgttg cccgtcgaag gtgagcggcg ccggcatcgg cagctctaac
    14521 acctcgagcc gcgctccatc ctcggcccgc gcgtcctgca ggcgctcgcg gttctcctcc
    14581 agcggcgcgt agttcggatc ggcggggttc tgctcgcgac acagcaccac ggtccgtggc
    14641 ccgacgaagc ggcacacgtc gtccacgtgt ccgtgcgtgt cgtcgcccgc gatgccgcgc
    14701 cccaaccaca cgacgttggt ggcgcccagc gcgtcgtgca acaccgcttc gacgtcggcg
    14761 cgcgtgaagc cggggttgcg ggtctgcacc accggatcga gcaggcactc ctcggtcgtg
    14821 agcaccgtcc ccgcgccgtt gacgtcgatc gaaccgccct cgagcacgac ccggcgctcc
    14881 ccccgcctga cgtcgaacag cgggaccccg agccggcgcg ccgcgagctc ggggagccgg
    14941 gcatccttcg cccagtcgcg gtatttcgcc caggcgttga agtggaagtg ggcgatcgcg
    15001 atctcggaac ggggtgcgtc gcgccggacg aagatcggcc ccgagtcgcg cgtccacccc
    15061 cggttggtgg gaaagcggag gaactggacc gcgtcgccgg ggaccgcgac gccggcgcgc
    15121 cgcagcacgc tggtggccga gcgctcgcgc gccggcgagt ccaccaggat gcgaaccgtc
    15181 tcgcccggcg cgaggtggcg cacgatctcg ccgtagaccc aggggatcgg ggcgaacttg
    15241 ccgggccagt cggtgcgatt gtgcgaccat ccgagccagg tggcggcgtg gcgctcccac
    15301 tccgcgggca tgcggtagcc gagcgccgcg ggagtcgctg cgcgtttcag gaggggtcgc
    15361 cgaggtaacg gcggtccagg ccgccgtagg cgtccacgcg ccggtcgcgc aggaacggcc
    15421 agttgcggcg gacctcttcg agccgcaccg ggtcgagggg ggcgacgagt atcgtctccg
    15481 cgtcggcggg cgcctcggcg acgaccacgc cgaacggatc ggccagaaac gaatggcccc
    15541 agaattcgag acccggaccc tggccgtcga gccgctccag ccccacccgg ttcaccgccg
    15601 ccacgtagca gccgttcgcg atggcgtgac tccgctggat ggtgcgccag gcatcgagct
    15661 gttgcgcgcc gacctgcgcc ttttccgcgg ggtgccaccc gatcgccgtc gggtacacga
    15721 ggatggacgc gccctggagc gccgtgagcc gggcgccctc gggataccac tgatcccagc
    15781 agaccagggt cccgatgcgt cccgccgcgg tgtcgaaggc cctgaagccc agatcgccgg
    15841 gggcgaagta gaacttctcg tagtaggacg ggtcgtcggg gatgtgcatc ttgcgataga
    15901 tgccggccac cctgccgtcg gcgtcgagca cggcggcggt gttgtgatac agccccggcg
    15961 cgcgccgctc gaacagcgac gcgacgatca cgatccccag ctcccgcgcc accgccccca
    16021 gggcctcggt agacgggccc ggcaccggct cggccagatc gaacagctga tgatcttcgc
    16081 gctggccgat atagagggtg cggaacagct cggggagaca gacgagcgtc gcgccgcgtg
    16141 ccgccgcttc gcggacccgg gtgatcgccc gctccaggtt cgcctgggga tcggccgtca
    16201 tccgcatctg caccaggccc actgtcgtgt ggcgcgccgt gccggcctgc ttcgtcatgg
    16261 ggaaagagta ccaatgcggc gcttcccgga ccagtgaccc gcccgcacca agtccagtct
    16321 acggcaccgc gtgcgattcg actcagtggc tgatcgcgaa gaaccaggcc gcgatcacgg
    16381 gcccccgcag accaccgaaa cccggctgcg gcgcaggagc gtccggcgcc gggggaggag
    16441 gcggaccgaa ttccggctga aacagatacg cgatgtgcct caccggatcc acgctgatcg
    16501 tcttggcacc gcgcatcgtg gggacggtgg cgaccaccct gtatgtgtcc ggcgagtcct
    16561 cgtgcgcgat ggtgacgctg ctgtcgcgcc ccgccgggat gtagatcagc ttctccgcgg
    16621 gatcccatcc gagggcatcg acgccttcac cgttcgcgat ggtcgccacc accttgcctg
    16681 acgcggggtc caccacgacg gaggtcctgc ggcaaccgga aaagatgcgg tgggtcttcg
    16741 catcgtaggc gatgccggtc gggccctggc agggggcgag cggccacgac gcccgcaccg
    16801 tcatggtctt cacgtcgatc acctgtatcg tgctcgtgcc ttcgttgttg acgaagagcg
    16861 tccccttgcc gtcgctcgcc gccccctcgg gcgaggtgct ctcgagctcg gcggtgccga
    16921 cgatggcgcc gctcccggca tcgagcgcca cggcggtgcc gaccgggtgg ctgtgattgg
    16981 tgagaatgat ccggtcggag aaggcgtcgt acatgatgcc gtccaggccg ccggtgggga
    17041 ccgggatctt cctgatcacg gccagggtgc gcaggtcgaa catggtgacg gtcgaatcgc
    17101 cgccgttggt ggtgaacccg tggtgcgacc tggggacgag cgcgatgccg tgcacccgcg
    17161 gggtatcggg aatgtcgccg agcaccttcc cggtggatcc gtcgaccacc atgacgtgtg
    17221 tgctgcggga gatgaagacg cgcccggtgc cgggctccgc ggtcaggtag tcggtgccgc
    17281 cgtcgccgcc gatgttgtat ttcgcgacct tgaacgtctg ggcagggctc gtacccggcc
    17341 aggccagcgc ggtgaccatg aggagcgtcg gaacgacgag cgggcggctg atcataggat
    17401 gctcgagtag tggaggccgg gttccaatct agacccaggg atagcggccc gccgtcaccg
    17461 tcaccaggac gcgcggctcg tcgtggagtc ggacgttccg gttaccgcaa tccgtacacg
    17521 acgacctggt tggaaaacgt gggcaggtac acgtgcccgt tggcgatcgt cggcgaggcg
    17581 aacttggcgt acgtgcccgc tccgtcacgg gcgaggtcct gatgattgtt ccagagctcc
    17641 cgggtgacgt cattcgcgtc gaaggctcgc aggatgccgg gactcacgtc gtgttccgca
    17701 tcgccgctga cggcgtacga tgcccagagt atcgccgtcc cgtccctgga actgtcggag
    17761 gaaaccgaga gcatggcccc gctgtgcccc gtcggcccac cggagccgaa cgccagctcc
    17821 gccagccgat ccagccggtt gctgccgcga tcgaacggga tggcgcggag cgggttgttc
    17881 tcgggccaca cgtagacgaa ttcccgcgcg cttccccggt agtaggccgg ctggcaatgc
    17941 atctcactga cggtcaacgg gacgacctgc tgcacctggt tggcggcggc ctggtacccg
    18001 cccatgtcgt tcgtattcag gagatacagg tacccgtcct tcccgccggt gagatagtag
    18061 ctcgaattgg gaatcagcag cgcgcccacg gagccgtagt ccacgtcgta gtcgttgaga
    18121 tactggtagt cgaagggcgt gaagtagctc gcgacctgca gcgtcgatcc ggacgggacg
    18181 agcttcaggg cgctctcccc ccgattcgtc aggctcgtgg ggtcacccga gtcccccacg
    18241 gtaccgttgc ccgccaccac atacaggctg ccggccgcat cggccgccat ccccattccg
    18301 ctttcccaca gaccgccggc gtatccgttg ggcgtcgcat tgtagaccac gcggggctgc
    18361 agggtaaccg cgtcgtatcc caggatccac ccgtgatacg gcccccagtc gcaatgggat
    18421 gcaaacgtca cgtatacgac gccgttcagc agcgtcagtc cctgccgctg gttctgccgc
    18481 tgcccatcga aggtgatcac gttggcgacg ctgccgtcgc cgctgccggg ataggtcgcg
    18541 gtgatccgca ccggactgcc ggtcgcttca ctcccgtcga cgaggctcac ggcgtgcagg
    18601 tgctgcacga aggcgttgcc gtcggtgctg cgggcgacga catacagggt tcgcctcgcc
    18661 gcatcgatca ccggcgtgcc gaccatgccg atgttgccgc tgaaatcccg gtacgagccg
    18721 ttgcaggccc ccgtcatgtc ggtattgcgc ggcggccgca tgccgggtgc cgaatagttc
    18781 ctggcccagt acagggtgcc gtcgtctccg tcgaaggcgt acagcgtatt gttgaccgtg
    18841 gcgatataga cgacgttacg ctccgcgccg ttgacgggga cccggcccac gaccagcggc
    18901 tgggcgtaca cctggtcatc cacggcgagg gtgaacacct tgccgaaatg ccgcaggttc
    18961 acgttgctcg gggtcaacgc ggtctcgtgg tcgttccatc ccgcccgggt gttgtcgttg
    19021 tgctgcgtga ggacggacac cgattgggga ttcggcgtcc gggtcgcggt catgtcgtcc
    19081 ttgcaggcgg cgaacgacac gacggcgagc gcgagcggca gtccgctgcc gaccaggagt
    19141 cgcccgcgtg cgatggtgtt gacggatgcg gtggcccggg cgctcatgac cacaccggca
    19201 ttcgcgatga gatcgtcctc cacgtgccgc tcctcacagg tgattgccca gagcgatcgg
    19261 tcgctcgaat tcgagggcga cgaccggggc cccccgtcta gggtcgcccg aaccggatcg
    19321 cctggctcgc gatcagccgg ccgctgtgag ccc
//
LOCUS       DBNGZW010000512         3807 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1343058, whole genome shotgun sequence.
ACCESSION   DBNGZW010000512 DBNGZW010000000
VERSION     DBNGZW010000512.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3807)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3807)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3807
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1343058"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(593..1090)
                     /locus_tag="AC16JC_12095"
     CDS             complement(593..1090)
                     /locus_tag="AC16JC_12095"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019003721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIR5165481.1"
                     /translation="MTPRPDPLPHLLLMRRALGLARQARDHGNHPFGALLTDADGRVR
                     LEAENTVVTDRDCTGHAEINLMRLACLDLDHDTLAAATLYTSTEPCAMCAGAIYWAGV
                     SRVVFGLREGELRILTGDDPKNPTLALPCGEVFARGQRPIEVIGPLLEDEARAVHDGY
                     WRALR"
     gene            complement(1382..2266)
                     /locus_tag="AC16JC_12100"
     CDS             complement(1382..2266)
                     /locus_tag="AC16JC_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165482.1"
                     /translation="MTLVTGTHQCPGVNWDWTTGPDGTLHLRDLPIECTDTTDDPRVS
                     GTHTAAANMDVWGTLDNGSGVQWASVRLENAGGAWEGRQLGVASLPGRGDIMVFWYRG
                     TGGYEGLSYFALVTGSEPWKIEGQIFPGDPPPPYAEGTTITRPQASGPVADDAVAVIT
                     GTADCPGLGFSFTADPNGIWHVRDDYHADRCVVTTDDPRVTGVRSSTWNFDVWGNPGA
                     GVGAGVQWGIPRLENDGGAWEGLATGVVSLPGRGDIIVNWYTGTGGYEGLGYFELWTG
                     SGPWRIQGQVFPGEPPTP"
     gene            2862..2999
                     /locus_tag="AC16JC_12105"
                     /pseudo
     CDS             2862..2999
                     /locus_tag="AC16JC_12105"
                     /inference="COORDINATES: protein motif:HMM:NF025637.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(3092..3655)
                     /locus_tag="AC16JC_12110"
     CDS             complement(3092..3655)
                     /locus_tag="AC16JC_12110"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HIR5165483.1"
                     /translation="MATITARNGVNVDQLVATIGAVQQDPNIAAFTFKASSTWRSGAR
                     NDGEIKDFIHAGQPAQRPAAFRLAGDEPPVLLGSNEGPNAVELLLQALGFCYAVGYIY
                     NAAARGIEISELRYEIEGDLDLHSFLGLEGPRAGFTAIRARSWVRSPNAGKEQLEELC
                     QYVQDTSPVRDCLANPIPVTTDLVVLD"
BASE COUNT          561 a         1294 c         1353 g          599 t
ORIGIN      
        1 gctcccgtgg ctcgggtggc tgcccgtcgc attctcggcc gtcagcctcg ggctcaacgc
       61 ggcttcgcgc agcgccggcg agcggcgcat ctgggtcccg gtggcgctgg tgttgctcgc
      121 ggccagcctc gcggtggcgc tgaccgccgg ttgacggggc ccgccgcggg ttgacgccgg
      181 ccgtgacgcc ggcgacccga cagcgcggag cgtctgggag ttcgccttgc ggctactgct
      241 cagcgacgtc gcgaacggcc cggatgaggt cctcggcctt ggcggcggga actcggaccg
      301 cgcacgacgc agccttcgtg ggcgtgcttc tggagccgga gggtctgtcc ctctggtctt
      361 tccttcacct cgcacggtcg agacccatgc ccagcacctg gtggacaagc tcgcggtcgg
      421 ctcacgtgcc gagatcgccg cgtggcacac gcgggaggtc gccggcaagc ctcccgctca
      481 gccgctgcgt tcgagtccgc gagcggcagc gaaggatctc aacacgatgg cacgatcgtc
      541 ttgatcaagg accgcgaagg cccgccctgc cctcgagtgt gactccgtac tgtcaacgta
      601 gagctctcca gtaaccgtcg tggacagctc gggcctcatc ctccagcaac ggtccgatga
      661 cttcgatcgg gcgctggcca cgggcgaaca cctcgccgca cggcagggcc agcgtcgggt
      721 tcttcggatc gtcgccggtc aggatccgca gctcgccttc acgcagcccg aacacgacac
      781 gcgacacgcc agcccaatag atggcgccgg cgcacatggc gcatggctcg gtgctggtgt
      841 acagcgtggc cgccgccagc gtgtcgtggt cgagatccag gcaggccaga cgcatcaggt
      901 tgatctcggc gtggccggtg cagtcacggt cggtgacgac cgtgttctcg gcttccagcc
      961 gcaccctgcc gtcggcatct gttagcagcg cgccaaatgg gtggttgcca tggtcgcggg
     1021 cctggcgggc gagccctaac gcccgacgca tcaacagcag atgaggcagg ggatcggggc
     1081 gcggagtcat ggctgcatta tcgggcgacc tcaagacagc cacgatcatg ggtcagcggg
     1141 cggcagagga cgtcggatcg acactatctg ctcatggtgt cgcgggcttc cggtgactga
     1201 ccgcacgcaa ctcgacgagg tcttcggcac ggatggggtg tccgacgacc gagggcgccg
     1261 tcgaacgtgt ggaaccgccg ggccaggcag cctgtggcga cgccttacgc cgctccgggc
     1321 gagccgaaac ggagcgcccg gcgcggagcc gggaggcgcg ggaggatcgg agcgatccgc
     1381 cctaaggggt ggggggttcg ccggggaaga cctggccctg gatcctccag ggaccgcttc
     1441 cggtccacaa ctcgaagtag ccaagcccct cgtatccgcc ggtccccgtg taccagttca
     1501 cgatgatgtc gccgcgcccg ggcagcgaga ccacgcccgt ggcgaggccc tcccaggctc
     1561 ccccgtcgtt ctcgaggcgc gggatgcccc actggacacc cgcaccgacg ccggcgcccg
     1621 ggttccccca gacgtcgaag ttccacgtgg aggagcggac cccggtgacg cgcggatcat
     1681 cggtcgtcac cacgcagcgg tcggcgtggt agtcatctcg gacatgccat atcccgttgg
     1741 ggtctgccgt gaagctgaag ccgaggccgg ggcagtccgc ggtgccggtt atcacggcca
     1801 ccgcgtcgtc ggcaaccggc ccgctggcct gcggccgcgt gatcgtcgtc ccctccgcgt
     1861 agggaggtgg agggtcgccc gggaagatct ggccctcgat cttccagggc tccgaacccg
     1921 tgaccagcgc gaagtaggac agcccctcgt agccgccggt ccccctgtac cagaacacca
     1981 tgatgtcccc gcgtccgggc agagaggcga ctcccagttg ccggccctcc caggctcccc
     2041 cggcgttctc gaggcgcacg ctggcccatt ggacacccga gccgttgtcg agcgtccccc
     2101 acacgtccat gttcgcggcg gcggtgtggg tgccgctgac gcgaggatcg tcagtcgtgt
     2161 cggtgcactc gatgggcaga tcccggaggt gcagagtgcc gtcgggaccg gtcgtccagt
     2221 cccagttgac gccggggcac tgatgggtgc ccgtgaccag ggtcaccggc tcgtgggtca
     2281 ctggggtcgg cgcgggggtc ggcgtcgggg tagcgacctc cgccgagggc acgctggtcg
     2341 gggtcggcga tgcgggcaat ggagtcgggc tcacgccggc gccacagccg gccagaaggc
     2401 caagggagat caggctgacc agcagctggc gaccgatgtc gcatccgccc atgtccgttc
     2461 tcccgggacg ctcgcggcgg gtcggtctcg ccagtggctc ggcgcggtcc cgggcggaca
     2521 aggtatggac cgcgtgtcac agcctcccgc tggcggtgac cctagccgct gatcgcggat
     2581 ctggcgagag tcgaacggcc ccccggcgcc cggtgcgctc tgagggttga ccccccaggc
     2641 gaagcaccgc gagcgaatag accgcgccag ccactcctcc gacgattcgg ggtgaagctc
     2701 tgcacggtat gtggtgtcgt caccggccgc gcgggtagcc gctgcgccgg gcacgcccgg
     2761 cagtccaacc gcagccggca caacgccctg accagcacgc gggcctggca gcggctgagc
     2821 gcccgcgtgc tgcgtgcctg gcgtggcgag cgcggtgacg ggtgcccagc tatcagcgca
     2881 aggcgcatcc agccagcgac ctgaccgtcg accatgtggt ccccctcgct gctggcggtg
     2941 ctccgttcga catcggcaac tgcgcggtgc tctgtcggtc atgcaactcg acgaagggcg
     3001 cgtcggagcc tgaccgggtg taccccacac gcgggagtgg acatcagcgc tcgcattccg
     3061 aggtaccggg cgggatgcga gcgtcatgca ctcagtcaag gaccacgagg tcggtcgtga
     3121 ccgggatcgg gttcgccagg cagtcgcgga ccggcgaggt gtcctggacg tactggcaca
     3181 gctcctcgag ctgttccttg ccagcgttcg ggctcctcac ccagctcctg gcccggatgg
     3241 cggtgaaccc ggcgcgcggg ccttccagcc cgaggaagct gtggagatcg aggtcgccct
     3301 cgatttcgta tcgcagctcg ctgatctcga tgccccgggc ggccgcgttg tagatgtagc
     3361 cgaccgcgta gcagaagccg agcgcctgga gcaggagctc gaccgcgttg ggaccctcgt
     3421 tcgagccgag gaggaccggc ggttcgtcgc cagcgaggcg gaacgccgcg ggccgctgcg
     3481 ccggctggcc ggcatggatg aagtccttga tctcgccgtc gttgcgggcg ccggagcgcc
     3541 aggtgctcga tgccttgaac gtgaaggccg cgatgttcgg gtcctgttgg acggccccga
     3601 tcgtggccac gagctgatcg acgttgaccc cgttccgagc ggtgatggtg gccatcgggc
     3661 tgcctcctgt cgtacggtca cctcatgtgg acgaccgttc aggggcagtg acgcctcgcc
     3721 ggccggaaga tattccttca ctgcatcctc ggccgagacg ggaataatcg gcccggtcgt
     3781 aacgtcaaga ccgagaagga tcggttc
//
LOCUS       DBNGZW010000513         2228 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_996159, whole genome shotgun sequence.
ACCESSION   DBNGZW010000513 DBNGZW010000000
VERSION     DBNGZW010000513.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2228)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_996159"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            229..1380
                     /gene="metK"
                     /locus_tag="AC16JC_12115"
     CDS             229..1380
                     /gene="metK"
                     /locus_tag="AC16JC_12115"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR5165484.1"
                     /translation="MAREEYLFTSESVTEGHPDKIADQISDAVLDAILAQDPTGRVAC
                     ETLLTTGLVVVAGEITTSCYVDIPKIARETIKAVGYTRAKYGFDYETCGVITAIDEQS
                     GDIAMGVDKLGAGDQGLMFGYACTETEELMPLPIMLSHKLVQRLTAVRKSGALDYLRP
                     DGKSQVSVRYVDGKPVSVETVVISTQHSPDVTLEKIRQDLVEQVVIPTIPKHLINPRR
                     ITYHINPTGRFVTGGPMGDTGLTGRKIIVDTYGGSCPHGGGAFSGKDPTKVDRSACYM
                     ARHVAKNIVAAGLAERAQVQVAYAIGVADPVSIMVETFGTGKAANAKLEQMIRRHFDF
                     TPAGIIKYLDLRRPIYRKTAAYGHFGRSEPEFTWEHTNRVKDLRDDAGI"
     gene            1498..1665
                     /locus_tag="AC16JC_12120"
     CDS             1498..1665
                     /locus_tag="AC16JC_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165485.1"
                     /translation="MTEDDVSDLSPAPAGKPKIERAEQQSLDSFASMKVRIGDLTPAQ
                     EERLTSWTHDT"
     gene            1734..>2228
                     /locus_tag="AC16JC_12125"
     CDS             1734..>2228
                     /locus_tag="AC16JC_12125"
                     /inference="COORDINATES: protein motif:HMM:NF015644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HIR5165486.1"
                     /translation="MANGRRSPRLGLWLLLAFILLAGLGQAVPLYTDWLWYGEVGFTQ
                     VFTTVLSLRGWLLLWAGGVVFLFMYANLWVAARTAAPDVLWELDDQLGLPGRAVLEPL
                     IRRLLLPVIAVIALLSGLRAGASWELVLGYVNATPFGVADPLFGRDISFFVFELPLWR
                     FLYGG"
BASE COUNT          353 a          757 c          760 g          358 t
ORIGIN      
        1 ttgtcagctg gccgctggcg ctgccgcccg gcggctcctg ggaaggcgag ctacgcatcg
       61 ttccgcgtgt gatccctctc gccggctgag cacgcggctg gttgagcgcg caggggaaca
      121 cggcgcgcgg gcatttccct tgaggcgtct ccgcgcccat gctactctgc cacaagcgcg
      181 tccggagacg gcgcgcgccg catccactac atgacgggga ggaaaccgat ggcgcgtgag
      241 gagtacctgt tcacttcgga gtcggtgacc gaagggcatc cggacaagat cgccgatcag
      301 atctccgacg ccgtcctgga cgcgatcctg gcccaggacc cgacgggccg cgtggcctgc
      361 gagacgcttc tgaccaccgg gctcgtcgtc gtggcgggcg agatcaccac atcctgctac
      421 gtggacattc cgaagatcgc gcgcgagacc atcaaggcgg tcggctacac acgcgccaag
      481 tacggattcg actacgagac ctgcggtgtc atcacggcca tcgacgagca gtccggcgac
      541 atcgcgatgg gcgtggacaa gctgggggcc ggcgaccagg gcctgatgtt cggttacgcg
      601 tgcaccgaga ccgaggagct gatgccgctg ccgatcatgc tctcgcacaa gctggtgcag
      661 cggctcacgg cagtgcggaa gagcggcgcg ctcgactacc tccgcccgga tggcaagagc
      721 caggtgtcgg tgcgctacgt cgacggcaag cccgtctcgg tcgagaccgt ggtcatctcg
      781 acgcagcaca gtcccgacgt gacgctggag aagatccggc aggatttggt cgagcaggtc
      841 gtgatcccga ccattccaaa gcacctgatc aatcccaggc ggatcaccta tcacatcaac
      901 ccgacggggc gcttcgtcac cggcggcccc atgggtgata cggggctgac ggggcgcaag
      961 atcatcgtgg acacctacgg cggctcctgc ccccacggcg gcggtgcgtt ttcgggcaag
     1021 gacccgacga aggtggaccg gtcggcctgc tacatggcgc gccacgtggc caagaacatc
     1081 gtggcggccg gtctcgccga gcgggcgcag gtgcaggtcg cgtacgccat cggcgtcgcg
     1141 gaccccgtgt ccatcatggt ggagacattc ggaacaggca aggcggcgaa cgccaagctc
     1201 gagcagatga tccgccggca cttcgacttc acgccggcgg ggatcatcaa atacctcgac
     1261 ctgcgccggc ccatctaccg aaagacggcg gcctacgggc acttcggccg cagcgagccc
     1321 gagttcacct gggagcacac gaaccgggtc aaggacctgc gcgacgacgc gggtatctga
     1381 gtcctcgccc acgagcggca gcgactgggc cccgcggccg gcacgagcga ccggcgcggg
     1441 gcccggctgc gaccggcgaa aaaaggccgg atatcgagcg ggtgaaagga cgggggcatg
     1501 actgaagacg acgtcagcga tctctcgccg gccccggcgg gcaagcccaa gatcgagcgg
     1561 gccgagcagc agtcgctgga ctctttcgcg tcgatgaagg tccggatcgg cgacctgacg
     1621 cctgcccagg aggagcgcct cacctcctgg acccacgaca cttaaccgcc tcaggcggat
     1681 tcgaccgcga cgcccccgga ggatgtgcgc cctcgggggc cggaggttct tccttggcga
     1741 acggacggcg gtctccccgg ttggggttgt ggctgcttct cgccttcatc ctgctggctg
     1801 gcttgggcca ggccgtgccg ctgtacaccg actggctctg gtacggcgag gtcggcttca
     1861 cgcaggtctt cacgaccgtc ctgtcgctgc gcggctggct cctcctctgg gccgggggcg
     1921 tcgtcttcct gttcatgtac gccaacctct gggtcgccgc ccgcacggcc gcgccggacg
     1981 tcctctggga gctcgacgac cagctcgggc ttcccggccg ggcggtgctc gaaccgctca
     2041 tccggcggct gctgctgccc gtcatcgcgg tgatcgcgct cctctccggc ctgcgcgccg
     2101 gcgcctcgtg ggagctggtg ctgggctatg tcaatgccac gcccttcggc gtcgcggacc
     2161 cgctgttcgg ccgcgacatc tcgttcttcg ttttcgagct gccgctctgg cgcttcctct
     2221 acggcggg
//
LOCUS       DBNGZW010000514         2904 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_659997, whole genome shotgun sequence.
ACCESSION   DBNGZW010000514 DBNGZW010000000
VERSION     DBNGZW010000514.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2904)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_659997"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..858)
                     /locus_tag="AC16JC_12130"
     CDS             complement(76..858)
                     /locus_tag="AC16JC_12130"
                     /inference="COORDINATES: protein motif:HMM:NF014460.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5165487.1"
                     /translation="MSSGPLNRIGSWAARWLSQMGALARFAFGGARAAARLPAAGRAV
                     VLRVTLNQIRFTAVHAMGLVILLSGILSFLVISQAVRELGQIGATGLIGTLMVVAIVR
                     ELGPLITALAIVGRSGTAIAAELATNTVMGEARALEGMGIDPRQYLVLPRFAGAVASV
                     FALLVLFDLVAIAAGFAAARINGMTGTRYAEIVLQSLSAHDVWLTVGKALAFGLIVGI
                     VPSYFGLSVRRAATDVPIAVSRATVASIVGVFLCSALFVLLG"
     gene            complement(866..1573)
                     /locus_tag="AC16JC_12135"
     CDS             complement(866..1573)
                     /locus_tag="AC16JC_12135"
                     /inference="COORDINATES: protein motif:HMM:NF015724.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HIR5165488.1"
                     /translation="MRMRSLVGLIGLGFSGGVAALAAQGGGQDTRPGIAVFAFANGGS
                     YGTQKEDYDALERGMAGMLISELGANPALRVVERENIQRLVDEQNLGASGRVDDQTAA
                     KIGKIVGARYAVMGSFIDLYGDFRVDVRVVNVETSEIIKTEKVSMQREHLFELVRNLA
                     GALNKDLNLPPLSRQVSEQRMSRQIPTEALTFYSRALLYHDRGNHAQARVMFQKAIDV
                     FPAYAEAKDGLQREPTS"
     gene            complement(1591..2469)
                     /locus_tag="AC16JC_12140"
     CDS             complement(1591..2469)
                     /locus_tag="AC16JC_12140"
                     /inference="COORDINATES: protein motif:HMM:NF049367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5165489.1"
                     /translation="MAYTVTFWGARGSIPTPGPHTARYGGNTSCVAVEGPGGQLVILD
                     AGTGIRGLGATLVERQNGAVQAEILLSHTHWDHIQGLPHFKPFFAPGNAVRIWGSKQG
                     AKSLDEILHLQMEPAVFPIPLTAVSAALAVEEVRPGGEFTVGGGAFRVQAMQIRHPGI
                     TLAYRLAPVAGGRSMAYVTDNELGTGGSYDTSPGWRRELVRFLNEVDLLIHDAMYTPA
                     ELASHRGWGHSSYEEAVELAAEAGAKRLVLFHHEPEHDDGAMDTLLAAARRAAGQHGG
                     KALDVVAAQEGMQLSL"
     gene            complement(2567..>2904)
                     /locus_tag="AC16JC_12145"
     CDS             complement(2567..>2904)
                     /locus_tag="AC16JC_12145"
                     /inference="COORDINATES: protein motif:HMM:NF024934.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HIR5165490.1"
                     /translation="GLLMQSQGIALAMLRELPRRLRRVDEKVGSLVLMDVNGRVARLL
                     LDLADEAGGERITRRLTHHTIAQMIGSSRETVSRTMRELVDKGYIEVSRRDIVIRDRS
                     ALEAAAGRS"
BASE COUNT          487 a         1045 c          938 g          434 t
ORIGIN      
        1 gcgagcgaga aaggggcgcg gctccgttct gtgagcggca cgttctcgaa taccagcgga
       61 gcagcggtca tgtgatcagc cgagcagcac gaacagggcg gagcacagga acacgccgac
      121 gatcgacgcg accgtcgccc gactcacggc gatggggaca tcggtggcgg cgcgccgcac
      181 cgacagaccg aaatagctgg gcacgatgcc tacgatcagt ccgaaggcca gggccttgcc
      241 caccgtcagc cacacgtcgt gcgcggagag cgactggagg acgatttcgg catagcgcgt
      301 gccggtcatg ccgttgatcc gggcggccgc gaacccggcg gcgatcgcca cgaggtcgaa
      361 cagcaccagc agcgcgaaca cgctcgccac cgcgccggcg aagcggggca gcacgaggta
      421 ctgccgcggg tcgatcccca tgccttcgag cgcgcgcgct tcccccatca ccgtgttggt
      481 cgcgagctcg gccgcgatcg ccgtgccgga gcgacccacg atcgcgaggg cggtgatcag
      541 cggtcccagc tcccggacga tggccaccac catgagcgtg ccgatcagcc cggtggcgcc
      601 gatctggccc agctcgcgca cggcctggga gatcaccagg aaggacagga tccccgagag
      661 gaggatcacc agacccatgg cgtgcacggc cgtgaaccgg atttgattga gcgtgacgcg
      721 caggaccacc gcgcgccccg cggccggcaa acgcgccgcg gcgcgggcac cgccaaaggc
      781 gaagcgcgcc agcgccccca tttgggagag ccagcgcgcc gcccagctgc cgattcggtt
      841 gagcgggccg ctgctcaccg gagcgtcatg acgtcggctc ccgctgcagc ccgtccttgg
      901 cctcggcgta tgccgggaag acgtcgatgg ccttctggaa catcacccgc gcctgggcgt
      961 ggttgccccg gtcgtgatag agcagggcgc ggctgtagaa ggtcagcgcc tcggtcggga
     1021 tctggcggct catgcgctgc tccgagacct gccgggacag cggcggaaga ttgaggtcct
     1081 tgttcagcgc gccggccagg ttgcggacca gctcgaacag atgctcgcgc tgcatcgaga
     1141 ccttttcggt cttgatgatc tccgacgtct cgacgttcac cacccgcacg tccacgcgga
     1201 aatccccgta caggtcgatg aagcttccca tgacggcgta gcgcgcgccc acgatcttgc
     1261 cgatcttggc ggcggtctgg tcgtccacgc ggcccgaggc gcccagattc tgctcgtcca
     1321 ccaggcgctg gatgttctcc cgctccacca cgcgcaacgc ggggttcgct cccagctcgg
     1381 agatcagcat ccccgccatg ccgcgctcga gcgcatcgta atcctccttc tgcgtcccgt
     1441 acgaaccgcc gttcgcgaac gcgaagaccg cgattcccgg ccgggtgtcc tggccgccgc
     1501 cctgcgccgc cagcgccgca acgccgccgc tgaagcccag cccgatcagg ccgacgagtg
     1561 aacgcattcg catacgccta gcacctcgat ctaaagagac agttgcatgc cttcctgtgc
     1621 cgccacgacg tccagcgcct tcccgccatg ctgcccggcc gcccgtcgcg ccgccgccag
     1681 cagcgtatcc atcgcgccgt cgtcgtgctc gggttcgtgg tggaacagca ccaggcgctt
     1741 cgccccggct tcggcggcca gctccaccgc ttcctcgtac gacgaatgtc cccagccgcg
     1801 atgtgacgcc agctccgccg gcgtgtacat ggcgtcgtga atcaggaggt ccacctcatt
     1861 caagaaccgg accagctcgc gccgccaccc gggcgacgtg tcgtaactcc cgcccgttcc
     1921 gagctcgttg tccgttacgt aggccatact gcgtccacct gctacaggcg ccaaccggta
     1981 agcgagcgtg atcccgggat gccgaatctg catcgcctgc acccggaacg cccctccgcc
     2041 gaccgtgaat tcaccgcccg gccgcacttc ctccacggcc agcgcggccg acacggcggt
     2101 gagcggaatc gggaagacgg cgggctccat ctgcagatgc aggatctcgt ccagcgactt
     2161 cgcgccttgc ttggaacccc agatgcgcac cgcattcccc ggagcgaaga agggcttgaa
     2221 gtgcggcaaa ccctggatgt ggtcccaatg ggtgtgcgac aggaggattt ccgcctgcac
     2281 ggcgccgttc tgccgttcca ccagcgtggc cccgagtccg cggatgccgg tgccggcgtc
     2341 gagaatcaca agctgaccgc cgggcccctc gaccgcgacg caggacgtgt tcccgccgta
     2401 gcgcgcggtg tgaggcccgg gagtcgggat ggaaccccga gcgccccaga aggtcaccgt
     2461 gtaggccatt cgatcgggct cgcccaaagc ggtgctaagt tacggaaaat cgggaaggta
     2521 ggacaagtcg acccctcacg ggtggccggt gaccctgctc acccggctac gaccggccag
     2581 ccgcggcttc caacgcagaa cggtcacgga tcacaatgtc gcgccgggac acctcgatgt
     2641 agcccttgtc cacgagctcg cgcatcgtcc ggctcacggt ctcgcgtgag gagccgatca
     2701 tctgggcgat ggtgtggtgg gtgaggcggc gcgtgatgcg ctcgccgccc gcttcatccg
     2761 ccaggtcgag cagcaggcgc gcgacgcgtc cgttcacgtc catcagcacc aggctgccga
     2821 ctttctcgtc cacacggcgc agccgccgcg gcagctcgcg cagcatcgcg agcgcgatcc
     2881 cctggctctg catcagcaac ccct
//
LOCUS       DBNGZW010000515         9370 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1057703, whole genome shotgun sequence.
ACCESSION   DBNGZW010000515 DBNGZW010000000
VERSION     DBNGZW010000515.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 9370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9370
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1057703"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..416)
                     /locus_tag="AC16JC_12150"
     CDS             complement(108..416)
                     /locus_tag="AC16JC_12150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HIR5165491.1"
                     /translation="MATATKTKLKVFPLADRVAIRPMEETEQMKGGLYIPDTAKEKPI
                     QGEVIAVGPGRTEKGEKVPMELKVGDRVVYGKYSGTQVELDGEEIILIKEADVIAKLS
                     "
     gene            complement(540..953)
                     /locus_tag="AC16JC_12155"
     CDS             complement(540..953)
                     /locus_tag="AC16JC_12155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165492.1"
                     /translation="MTAPLPRWGTLGSGALILVLLACASARYRMPSALHSYAYYVAGH
                     DSLSVALEAALRRRGLEVVPQVRGGAGPTVAVIHFTFRDPGDSLGAVLHVQLADTRTG
                     VVVGDGMAVLAGLPPALSARAEALLDSIGLRRPSP"
     gene            complement(950..1966)
                     /locus_tag="AC16JC_12160"
     CDS             complement(950..1966)
                     /locus_tag="AC16JC_12160"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR5165493.1"
                     /translation="MRTTERLLILALSAAGVVCGARPLVAQRDAGLLYGRWTPGGITT
                     YEARLDRPLGTWLRHGPTLHVLLEDRAAGRGFYGLGYEVASPRGGSGVAVYGLLGAAL
                     GMISDTGGQGLGALWSAGLGVEWRPLRFLGLNLEERYHVTDRGPHGFWHPGAPHKGWG
                     TLLGLSVTLESGVRRRPAAVSSGAVPAPSPSPGRGDGGERPADPPLMIVGRAGDVVQT
                     ALAVLGSPYQWGGTAENGFDCSGLVQYAYARHGIRLPRTSRAQSQSGTPVAAVSDSLR
                     AGDILTFAARPGGGVTHVGLYVGDGKFIHSSSTGVKLSRLSSADPEAAYWIPRWVGVR
                     RVLP"
     gene            complement(1953..2249)
                     /locus_tag="AC16JC_12165"
     CDS             complement(1953..2249)
                     /locus_tag="AC16JC_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165494.1"
                     /translation="MSAAGLPRWLARALVVIGWLLTPLWAWGASFLGLWAGAHLAGRF
                     TSPVAMLAPALLGAVLCGFVTLWIWVRFMRRVPHLLSHHMAERPSVEQPPLADH"
     gene            complement(2253..2864)
                     /locus_tag="AC16JC_12170"
     CDS             complement(2253..2864)
                     /locus_tag="AC16JC_12170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165495.1"
                     /translation="MTTSAVPTADRSDFRNVLAAGTLVGAITGIGVVAFVAISRLLPG
                     GAARDILESALVLAGGVAVSMLPAQRAVSRTAEGVAGAAAIGLWGTVVFMAIDIVLLR
                     PFKAYPWTWDAIGGGSTWWYLPIWWMLGTFLAWMGGWLTAARNARGAASVAQVALPVV
                     VGALVLTAAGKALGCPVPLAALTGATYTVTLAGLAVFFNARRA"
     gene            complement(2921..3706)
                     /locus_tag="AC16JC_12175"
     CDS             complement(2921..3706)
                     /locus_tag="AC16JC_12175"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HIR5165496.1"
                     /translation="MLLALDVGNTETTVGLFQADRLVDHWRLTTTTARTPDEWGALLS
                     TYLTNAGHSPSGVRAVVIASVAPLVTQSICEGVAAATGIMPVQVDARSPLPITLDVDE
                     PLTVGADRIVNTLAAARVFARDTIVVDFGTATTFDCITADGRFIGGVIMPGIRTASDE
                     LVRRTAKLPATELTPPERVIGRRTEECIRAGVLWGTADAVDGIIRRIRSEWPTKGEKP
                     PYVVATGGLAPLVAPLTQGIEAVRPDLTLVGLRLAATALGLGW"
     gene            complement(3760..4509)
                     /locus_tag="AC16JC_12180"
     CDS             complement(3760..4509)
                     /locus_tag="AC16JC_12180"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin--[biotin carboxyl-carrier
                     protein] ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR5165497.1"
                     /translation="MIGTLDGLPGAVLARRWGVPQCRVAREAASVLDSAHALGGEGGP
                     AGTILLADVQTAGRGRNGRTWYSPPGGVWLALLLRPRTAGLGVMAIRAGLVVADVVDE
                     LLGASLARIKWPNDVLLDDRKLAGVLCEGRWQGDALQWLAVGIGVNVTNEIPRALRGN
                     AVALHELLPGVRRLDVLDRLVPPLVRLGTGGDRLSERECTAFAARDWLRDRLLLAPAP
                     GRAAGLRPDGALLVEGADGQFVVRDGHVEAA"
     gene            complement(4506..5330)
                     /locus_tag="AC16JC_12185"
     CDS             complement(4506..5330)
                     /locus_tag="AC16JC_12185"
                     /inference="COORDINATES: protein motif:HMM:NF013226.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HIR5165498.1"
                     /translation="MTEVWRVTRGHNLAIAAAGVLAGGWIGLGTIAVPKLLVFAALSG
                     VGLGAAGNVANDLADASLDRLNPAAAGRPLAAGRMPRETAHLLVWLGALGGVSAAALV
                     SGRQVLAGLIALLVMLGYSPFLKRHGLPGSLAVAGIAGRPLFYGALAVDRPAAGLVPW
                     VLAAWIHLARELAKDLQDEPGDRARGRRTLPVRLGRAGAARIAWWCCLAFVPLSIALP
                     LLARYGAVYFGVAVAAQLLVLVAGRGLLRERFARASLLLKVAMVVGLAALVLGGIA"
     gene            complement(5323..6447)
                     /gene="bshA"
                     /locus_tag="AC16JC_12190"
     CDS             complement(5323..6447)
                     /gene="bshA"
                     /locus_tag="AC16JC_12190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009621053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HIR5165499.1"
                     /translation="MKIGITLYPTYGGSGAVATELGLELARRGHEVHFISYASPFRLR
                     GVTERVTFHEVVPADYPLFEQSQYAFALAVKQHEVALREELQVMHVHYAIPHAATAWL
                     AKQMLAEERDLKLVTTLHGTDITLVGKDPSYFSLTKFSIEHSDRVTAVSAFLRDETHQ
                     FFGCDTCEIVVIPNFINRTEYFPPRDPAARCRLAPADHRVIMHISNFRPVKRVQDVVR
                     VFAGVRKTMKATLVLAGDGPDHDAAQREAARLGVKRDVRFTGRVDDVADILRGADLFL
                     LPSETESFGLAALEAMACGVPVIASRTGGLPEVVEDGVSGFLVPVGDVAGMTERAVEL
                     LKDPARHAAVQREALRRAGEFSSDRIVPQYEQLYEDVLRD"
     gene            complement(6464..7399)
                     /gene="miaA"
                     /locus_tag="AC16JC_12195"
     CDS             complement(6464..7399)
                     /gene="miaA"
                     /locus_tag="AC16JC_12195"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HIR5165500.1"
                     /translation="MLVGPTASGKTALAAALAERIPLTVISADARQVYRRLDIGTAKP
                     DAELRVRVPHRGIDLVEPGERYSAGRFARDAAGWLTEARAAGRLPLVVGGTGFYVRAL
                     ADGLFQEPPLDPARREQLSAWTAGLGGSDVARWAARLDAQFTGGGRQRAARSVEVALL
                     TGRPLSLWQRMARAAGVMRPWYILLTLPRELLRRRIAERVDAMLAAGLVEEVRAELAR
                     GTAPDAAGLDGLGYRETVAMLLGKLDPAELREAIVRATRRYAKRQETWFRHQLRYRLS
                     AVGYQPEEVWTLDATRTSASLAGEVLDRWRVVSGR"
     gene            complement(7489..9165)
                     /gene="mutL"
                     /locus_tag="AC16JC_12200"
     CDS             complement(7489..9165)
                     /gene="mutL"
                     /locus_tag="AC16JC_12200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HIR5165501.1"
                     /translation="MSDRISVLPPHVADQIAAGEVVERPASVVKELVENALDAGARSV
                     RVELEDGGRALIRVSDDGSGMSPTDARLALQRHATSKIRNAADLIGVGSYGFRGEALP
                     AIASVSRFELETADGSGPGIRVSVSGATVEREEPAVRQQGTTVTVRGLFFNTPARRKF
                     LRSPAAETRATSEVVAVMALARPDVAFRLTSDGRTLLDAGAASRSIDRIHALWGRDLA
                     DTLLPVAHRRGAVEVTGFAQRPSQARPAGRKGYVFVRGRPVRDPFMLRAAEAGYRSAI
                     APGDRPTLILFLDLPGDAVDVNVHPAKAEVRFRERMQMEQVVEEAVRAALAPLDAAAP
                     IGTAAPLEAAAWDGTGSIPAGVPTELFSAEGMGGRPRGPELLQVFDTYLMFQTEDGVA
                     IVDQHSAHERVLYETVMRQLGGDGAPAQRLLLPLTLEFTPPELEAIEANRELLQRVGY
                     ELEPFSGRTVVAHTAPNPHPRFEAARCLQELVSDLAGGRFGGWANRLERFAATFACRA
                     AIKAGHHLDPEEARELVRRLLAATLPAHDVHGRPTIVQLPKGELERRFGR"
     gene            complement(9162..>9370)
                     /locus_tag="AC16JC_12205"
     CDS             complement(9162..>9370)
                     /locus_tag="AC16JC_12205"
                     /inference="COORDINATES: protein motif:HMM:NF018095.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR5165502.1"
                     /translation="RRARPVGVIVSAATPVRAAPYGSASASIVLGPGVAVLVEDEFNG
                     GQWLRVRRPDGVQGWVQAEQVARL"
BASE COUNT         1507 a         3533 c         3122 g         1208 t
ORIGIN      
        1 gtcaccttca ccgcctcggc cagctgatcc acgccccgct tcaaacggga gcgggcctcg
       61 gtgttgaact ccagatactt ggcagccatc tattcctccc ggcctggtta gctgagcttc
      121 gcgatgacgt cggcttcctt gatcagaatg atctcttccc cgtccaactc gacctgcgtg
      181 ccgctgtact tcccgtagac cacgcggtct cccaccttca gctccatggg gaccttctcc
      241 cccttctccg tccggccggg accgaccgcg atcacctcac cctgaatcgg cttctccttc
      301 gccgtgtcgg ggatgtacag tccgcccttc atctgctcgg tttcttccat gggcctgatc
      361 gccacccgat cggccagcgg gaagaccttc agcttcgtct tcgtcgcagt agccataaac
      421 gctctgcctc cacctgaagt tgaatgacgt aaagcattgt ggcatatcga cgttagcact
      481 ctcatgcatt gagtgccaag ggtcgccgcg gggcaatttc tcctcgcccg gcggcgctgt
      541 caaggggagg gtcggcgcag tccgatggaa tccagcagcg cctcggcccg ggcgctcagc
      601 gccggcggca ggccggcgag caccgccatc ccgtcaccga cgacgacgcc ggtgcgagta
      661 tcggccagct gcacgtgcag caccgcaccg agggaatcac ctggatccct gaaggtgaag
      721 tgaatgacgg ccaccgtagg ccccgccccg ccccggacct gcggcaccac ctcgagcccg
      781 cgacgtcgca gcgccgcctc cagtgccacg ctaagggaat cgtggccggc gacgtagtag
      841 gcataactgt gcagcgccga cggcatgcgg tagcgcgcag aggcacacgc cagcaagacg
      901 aggatcagcg ctccgctccc caacgtcccc caccgaggca gtggagcggt cacggcagga
      961 cccgccgcac ccccacccag cgcggaatcc agtaggccgc ttcgggatca gccgaggaga
     1021 ggcgggacaa cttcacgccg gtggaggagc tgtggatgaa cttgccgtct ccgacataca
     1081 gcccgacatg cgtcacgccg ccgccagggc gcgcggcgaa cgtcaggatg tcccccgccc
     1141 gcagtgaatc ggacaccgcc gcgacgggcg tacccgactg cgactgggca cggctggtgc
     1201 gcggcaaccg gatgccgtgc cgggcgtacg cgtactgcac cagcccggag caatcgaagc
     1261 cgttctcggc cgtgcctccc cactggtagg ggctgcccag cacggcgagg gccgtctgca
     1321 cgacgtcccc ggcgcgaccg acgatcatga gcggcgggtc cgcgggccgc tctcctccat
     1381 cccctctgcc cggggacggg gacggtgccg gcacggcgcc gctcgagacg gccgccgggc
     1441 gccgccgcac gccactctcc agcgtgaccg agagtccgag cagtgtcccc caccccttgt
     1501 gcggagcgcc ggggtgccag aagccatgcg gaccccggtc ggtcacgtgg tatcgctctt
     1561 cgaggttcag tcccaggaaa cgcagtggtc gccactcgac tcccaggccg gccgaccaca
     1621 atgctcccag tccctgaccg ccggtgtccg agatcatgcc gagcgcggcc cccaacaaac
     1681 cgtacaccgc gaccccgctg cccccgcgag gcgaggctac ctcgtagccg agcccgtaga
     1741 atccgcgtcc ggccgcgcga tcctcgagca gcacatgcag ggtcggacca tgacgcaacc
     1801 aagtgccgag cggccggtcg aggcgggcct cgtaggtcgt gatgccgccg ggagtccacc
     1861 gcccgtacag cagaccggca tcgcgctgcg ccacgagggg ccgtgcgccg cacaccactc
     1921 ctgccgccga gagggcgaga atcaggaggc gctcagtggt ccgcaagcgg aggttgctcc
     1981 accgagggac gctcagccat gtgatgcgaa agcaagtgag gcacacgtcg catgaaccgc
     2041 acccagatcc agagagtgac gaatccgcac agcacggctc ccagcagcgc cggcgcgagc
     2101 atcgccaccg gactggtgaa ccggccggcg aggtgagcac cggcccacag ccccaaaaac
     2161 gaggctcccc aggcccagag cggcgtgagg agccatccga tgaccacgag cgcgcgcgcc
     2221 agccagcggg gcaaccccgc agcgctcacg cgctacgccc tccgggcgtt gaagaacacc
     2281 gccagtccgg cgagcgtgac cgtatacgtg gcccccgtga gcgcggcgag cggcacgggg
     2341 caccccagtg ccttgcctgc ggccgtcagc accagggcgc cgaccaccac ggggagcgcc
     2401 acttgcgcca cggaggcggc gccgcgggcg ttccgcgccg ccgtcaacca cccgcccatc
     2461 caggccagga aggtgccgag catccaccag atcggcaggt accaccaggt gctgccccca
     2521 ccgatggcgt cccacgtcca gggatacgcc ttgaacggcc gtagcagcac gatatcgatg
     2581 gccatgaaca ccaccgtgcc ccagagcccg atcgcggcgg ctccggcgac tccctcggcg
     2641 gtacgcgaga ccgcgcgctg cgcgggaagc atgctcacgg cgacgccgcc cgcgagcacg
     2701 agcgcgctct ccaggatgtc ccgcgcggcg ccgcccggca ggagccggga gatcgcgacg
     2761 aacgccacga cgccgatgcc ggtgatggct ccaaccaggg tgcccgccgc gaggacgttg
     2821 cggaaatcgg agcgatcggc ggtcggtacg gcgctggtgg tcatgtagct ggctcttttc
     2881 gaggtatccg gttgatggtg cctaatttag cgcccctgcc ctaccagcca agccccagcg
     2941 ccgtcgccgc caggcggagc cccaccagag tgagatcggg ccgcaccgct tcgatcccct
     3001 gtgtcagcgg tgccaccagc ggggcgagtc caccggtcgc cacgacgtag gggggcttct
     3061 cacccttcgt cggccactcc gaccggatac gccggatgat gccgtccacg gcatcggccg
     3121 tcccccacag cacgccggcg cggatgcact cctcggtccg gcgcccgatg acccgctcag
     3181 gcggggtgag ctccgtggct ggaagcttgg cagtccggcg caccagttcg tcagacgcgg
     3241 tgcggatacc cggcatgatg acgccgccga tgaaccggcc atcggccgta atgcagtcga
     3301 aggtcgtcgc cgttccgaag tccacgacga tcgtgtcgcg ggcgaagacg cgcgccgccg
     3361 cgagcgtgtt gacgatgcga tcggctccga cggtcagcgg ctcgtccaca tcgagggtga
     3421 tcgggagggg cgaacgggcg tccacctgga ccggcatgat tccggtcgcg gcggcgacgc
     3481 cctcgcaaat gctctgcgtg accaacggag ccaccgaggc gatgacgact gcccggacgc
     3541 ccgagggcga atggccggcg ttggtgaggt aggtcgagag cagcgcgccc cactcgtcgg
     3601 gcgtgcgcgc agtggtggtc gtgagccgcc aatggtccac gaggcgatcg gcctgaaaca
     3661 ggccgacggt cgtctcggtg ttcccgacgt cgagggcgag gagcatcctg aaaactagac
     3721 gctcaggcgc ggagacgcga agctgctcag aaaagacgct caggccgctt cgacatgacc
     3781 gtcccgcacg acgaactgcc cgtccgcccc ttccaccagc agcgcgccgt cggggcgcag
     3841 gcccgcggcc cggccgggcg caggagcgag cagcaggcgg tcccgcagcc agtcgcgcgc
     3901 cgcgaacgcg gtgcattccc gctcgctcag ccgatcgccg ccggtcccga gacggacgag
     3961 cggagggacg agccgatcca gcacgtcgag ccggcgcacg ccggggagca gctcgtggag
     4021 ggcgaccgca ttcccccgca gcgcccgcgg aatctcattg gtcacgttca cgccgatgcc
     4081 taccgccagc cactgcagcg cgtcgccctg ccagcgtccc tcgcacaaca ccccggcgag
     4141 cttgcggtcg tcgagcagca cgtcgttggg ccacttgatg cgcgccagcg atgcgcccag
     4201 cagctcatcc accacgtcgg ccacgaccag gccggcgcgg atggccatca cgcccagccc
     4261 ggcggtgcgc ggccgcagga gcagcgccag ccagacgccg cccggtgggg agtaccaggt
     4321 gcggccgttc cgcccccgcc ccgcggtctg cacgtcggcc aacaggatcg tgcccgcggg
     4381 cccgccctcc cctccgaggg catgcgccga atcgagcacc gaggccgcct cccgcgccac
     4441 ccggcactgg ggcacccccc aacggcgcgc gagcaccgcg ccgggcagac cgtcgagcgt
     4501 cccgatcacg caatacctcc gagaacgagc gccgcgagac cgacgaccat ggccaccttg
     4561 agcagcaggc tggcgcgggc gaaccgctcg cgcagcaatc cgcgtccggc caccaggacg
     4621 agcagctggg ccgccacggc cacgccgaag tacaccgcgc catagcgcgc caatagcggt
     4681 agggcgatcg acagcggcac gaaggcgaga cagcaccacc acgcgatccg ggcggcgccg
     4741 gcgcgcccca gccgcaccgg gagcgtgcgg cgtccccggg cccggtcacc cggctcgtcc
     4801 tgaagatcct tcgccagctc gcgcgccagg tgaatccacg ccgcgagcac ccaggggacg
     4861 agaccggccg ccggccggtc cacggccagg gccccgtaga acagcggccg cccggcgatg
     4921 ccggccaccg cgaggctccc cgggagcccg tgtcgtttga ggaacggcga gtagccgagc
     4981 atcacgagga gggcgatgag ccccgccagg acctgacggc ccgacacgag cgcggcggcg
     5041 ctgaccccgc cgagcgcccc gagccacacc agcaggtgag ccgtctcgcg gggcatccgc
     5101 ccggcggcga gcggtcgtcc cgccgcggcg ggattcagac ggtccagcga ggcatcggcg
     5161 agatcgttgg cgacgttgcc cgccgcgccc aggcccacgc ccgacaaggc ggcgaagacg
     5221 agcagcttcg gcacggcgat agtcccgagg ccgatccatc cgccggcgag gacgccggcc
     5281 gccgcgatcg ccaaattgtg accccgcgtg acccgccaca cctcagtcac gcaggacgtc
     5341 ctcgtagagc tgctcgtatt gcgggacgat ccggtcggac gagaactccc ccgcgcggcg
     5401 cagcgcttcc cgctgcaccg ccgcgtggcg ggcgggatcc ttgagcagct cgaccgctcg
     5461 ctcggtcatg cccgccacgt cgccgacggg aacgaggaac cccgacaccc cgtcttcgac
     5521 gacttcgggg agcccgcccg tccggctcgc gatcaccggc acgccgcagg ccatcgcttc
     5581 gagcgcggcc aggccgaagg actcggtctc cgaaggcaac aggaagagat ccgcgccgcg
     5641 caggatgtcg gcgacgtcgt ccacgcgacc ggtgaaccgg acgtcccgct tgacgccgag
     5701 cctggccgct tcccgctggg ctgcatcgtg gtcggggccg tcgcccgcca gcacgagcgt
     5761 cgccttcatc gtcttgcgga cccccgcgaa cacgcgcacc acgtcctgca cgcgtttcac
     5821 gggccggaag ttcgagatgt gcatgatcac ccggtggtcg gccggggcca gccggcagcg
     5881 ggcggccggg tcgcgtgggg ggaagtactc ggtgcgattg atgaaattgg gaatgacgac
     5941 gatctcgcag gtgtcgcacc cgaagaactg gtgcgtctcg tcgcgcagga acgcggacac
     6001 ggcggtcacg cggtccgagt gctcgatgga gaacttggtg aggctgaagt aggacggatc
     6061 cttccccacc agggtgatgt cggtgccgtg cagcgtcgtg accagcttga gatcgcgctc
     6121 ctcggcgagc atctgcttcg cgagccacgc ggtggcggcg tgcgggatcg catagtgcac
     6181 gtgcatcacc tgcagctcct cgcgcaacgc cacctcgtgc tgcttcaccg cgagggcgaa
     6241 ggcgtactgg gattgctcga acagcggata gtccgcgggc acgacctcgt gaaacgtcac
     6301 ccgctcggtg acgccacgca ggcgaaacgg gctggcgtag gagatgaaat ggacctcgtg
     6361 gccgcggcgc gcgagctcga ggccgagctc ggtcgcgacg gcgcccgatc cgccgtaggt
     6421 gggatagaga gtgatcccga tcttcatggc tatcggctat cagctaccgg cctgaaacga
     6481 cgcgccaacg atccagcacc tctcccgcga gacttgctga ggttctcgtc gcatccagag
     6541 tccagacctc ctccggctga tagccgacag ccgatagccg atagcgcagt tgatgtcgaa
     6601 accacgtctc ctgccgcttg gcgtagcggc gcgtcgcacg cacgatcgcc tcgcgcagct
     6661 cggcggggtc gagcttcccc aagagcatcg cgaccgtctc gcggtagccc aggccgtcca
     6721 gcccggccgc gtcgggggcg gtgccgcgcg ccagctcggc gcgcacctcc tctaccaggc
     6781 cggccgcgag catcgcatcg acgcgctccg cgatgcggcg tcgaagcagc tcgcgcggca
     6841 gcgtcagcag aatgtaccac ggccgcatca cgccggcggc gcgcgccatc cgctgccaca
     6901 gcgagagcgg ccgtcccgtg agtagcgcca cctccaccga gcgcgccgcc cgctggcggc
     6961 cgccgcccgt gaactgcgcg tcgagccgcg ccgcccagcg cgccacgtcc gatccgccga
     7021 ggcccgccgt ccaggcgctc agctgctcgc ggcgcgcggg atcgagcggc ggctcctgga
     7081 acaagccgtc ggcgagcgcg cgcacgtaga atcccgtgcc gcccaccacg agcggcagcc
     7141 gtcccgccgc acgggcttcc gtgagccagc cggcggcatc gcgcgcgaac cgtcccgcgc
     7201 tgtaccgctc gccgggctcg accagatcga tcccccggtg cggcacgcgc acgcgcagct
     7261 cggcatcggg cttcgcggtc ccgatgtcga gccggcggta cacctgccgc gcgtcggccg
     7321 agatcaccgt gagcgggata cgctcagcca gtgcggccgc cagggcggtc ttgccactcg
     7381 ccgtgggccc gaccaacacc ggagtgagcg ggcggtcagg cggtcgggcg gtcggatgca
     7441 ccacggtcct gaccgcctga ccgcctgtcc gcccgaccgc cgctcgcctc atctcccgaa
     7501 cctccgttcc agctcgccct tgggcagctg cacgatggtc ggccgaccat gtacgtcgtg
     7561 cgcaggcaag gtagccgcga gcaagcgccg caccagctcc cgcgcctctt cgggatcgag
     7621 gtgatgcccg gccttgatcg ccgcgcggca ggcgaaggtc gcagcgaaac gctccagccg
     7681 gttcgcccac ccgccgaagc gtccgcccgc caggtcgctc accagctcct gcagacaacg
     7741 ggcggcctcg aagcgcggat gcggattggg cgcggtgtgc gccaccaccg tgcgccccga
     7801 aaacggctcc agctcgtagc ccacgcgctg caacagctcc cggttggcct cgatcgcttc
     7861 cagctccggt ggcgtgaact cgagcgtcag gggcagcagc agccgctgcg cgggcgcacc
     7921 gtcgcctccg agctgccgca tcaccgtttc gtagagcacc cgctcgtgcg ccgaatgctg
     7981 atccacgatc gcgacgccgt cctcggtctg gaacatgagg tacgtgtcga acacctggag
     8041 cagctcgggt ccgcgcggcc ggccacccat cccttccgcc gagaagagct ccgtcggaac
     8101 gccggcggga atcgacccgg tcccgtccca cgcggccgct tccagcggcg cagccgtccc
     8161 gatgggcgcc gccgcatcga gcggggcgag cgccgcccgc accgcctcct ccaccacctg
     8221 ttccatctgc atgcgctcgc ggaaccgcac ctcggccttg gcgggatgga cgttcacgtc
     8281 caccgcgtcg cccggcaggt cgagaaagag aatcaacgtg ggacggtcgc ccggcgcgat
     8341 cgcggagcga taccccgctt cggccgcccg cagcatgaac ggatcgcgca ccggcctccc
     8401 gcgcacgaac acgtacccct tccggccggc cgggcgggcc tgcgacggcc gctgggcgaa
     8461 ccccgtcacc tcgaccgctc cgcgacggtg cgccaccggc agcagcgtgt cggccaggtc
     8521 gcggccccac agcgcatgga tgcggtcgat ggagcgggac gccgctccgg cgtcgagcaa
     8581 cgtccgcccg tcgctcgtca gccggaacgc cacatcggga cgggcgagtg ccatgacggc
     8641 cacgacctcg ctcgtcgcgc gcgtttccgc ggcgggcgag cgcagaaact tgcgacgggc
     8701 cggcgtattg aagaacagtc cccgcaccgt caccgtggtg ccctgctgac ggaccgccgg
     8761 ctcctcgcgc tccacggtcg ctccgctcac cgagacccgg atccccggcc cgctgccgtc
     8821 ggccgtctcc agctcgaagc gcgacaccga cgcgatggcc ggcaacgcct cgccgcgaaa
     8881 tccgtacgac cccacgccga tcagatcggc cgcattccgg atcttgctcg tggcgtggcg
     8941 ctgcagtgcc agccgcgcgt ccgtggggga catgccggat ccgtcatccg agacccggat
     9001 cagcgcgcgt cccccgtctt ccagctccac ccggacgctg cgcgcaccgg cgtcgagcgc
     9061 attctcgacc agctccttca ccacggaggc gggccgttcg accacctcgc ccgcggcgat
     9121 ctggtccgcg acgtgcgggg gaaggacgga gatgcggtcg ctcacaggcg cgccacctgc
     9181 tccgcctgca cccacccctg gacgccgtcc ggccgccgga cgcgcagcca ttgtccgccg
     9241 ttgaactcgt cttccacgag cacggccacc ccgggcccga gcacgatgct cgcgctcgcc
     9301 gagccgtaag gtgccgcacg gacgggcgtc gcggcgctga caatcacccc cacggggcga
     9361 gcgcgacggt
//
LOCUS       DBNGZW010000516         2387 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1209594, whole genome shotgun sequence.
ACCESSION   DBNGZW010000516 DBNGZW010000000
VERSION     DBNGZW010000516.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1209594"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="AC16JC_12210"
     CDS             <1..1300
                     /locus_tag="AC16JC_12210"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR5165503.1"
                     /translation="AIPSVSTDPAHAADVRKATTWVAAKLAAAGPFTVRTMPTPGASV
                     VYAEWLGAAGAPTVLIYGHYDVQPEDPVEQWVSPPFTPTLRDGRLYARGVSDDKGPLL
                     IPIEVAEAFFATHARLPINVKFMLEGEEEVGSQHLEGFIAEHKDLLAADVVLSADGAM
                     WRSNEPSLTVANRGLAGLELTLRGPAKDLHSGRHGGSVANPLHAMARLIASLHQPDGR
                     VAVAGFYDRVRELAPPERAEIAGLPFDEAAYLAQIGAPAATGEPGYTTLERQWTRPTL
                     EVNGLWGGYQGPGMKTVVPGEAHAKITCRLVPDQDPDEVVALVTRHLERHVPPGTTLT
                     IRPGDHGARPARIARDHPALQAAEAALAEVYGVRPLIVRMGGTVPVAEIFQRLMGLDT
                     VYFSFSTGDEDFHAPNEFFRVHRLHEGLAAWARLWERLGR"
     gene            1297..>2387
                     /locus_tag="AC16JC_12215"
     CDS             1297..>2387
                     /locus_tag="AC16JC_12215"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5165504.1"
                     /translation="MTLQQEEAKVEAHSAALKKVLGVRDLALTQILFIIGLTWIGVAG
                     KLGPSHVVLWLLAVVLFYLPLTGVVIWLNQLMPLEGGLYQWAKLGFNQTIGFMVAWNL
                     WLYVILFASELGLTVATYLSYALGPSAAWMTTSAWFVSLASALATALLALLAFVGLSI
                     GKWVQNAGGVFMITIFSVLILLPFLGLATGHLESYHPFRTEIPAFSLFNLNVLGKMCV
                     GAFAGFEYMAILAGETHAPASTVKKSVYYAAPVITLFFILGTSTVVAYIPRDDIDLIG
                     PLPQVLRVGFGPFGIAGTLVTIAIVMTLVMRVSQISVAFTAVTRLPMVAGWDRLLPAW
                     FSRLHATHRTPVNSILVVAVFSFAMSVISL"
BASE COUNT          346 a          831 c          809 g          401 t
ORIGIN      
        1 cgccatcccg agcgtcagca ccgatcccgc gcacgcagcc gacgtgcgca aggccaccac
       61 ctgggtcgcc gcgaagctcg cggccgccgg accgttcacc gtccggacga tgcccacccc
      121 gggggcgagc gtcgtgtacg ccgagtggct cggcgcggcc ggtgcaccga ccgtgctgat
      181 ctacgggcat tacgacgtgc agcccgaaga cccggtcgag cagtgggtga gcccgccttt
      241 cacgccgacg ctacgtgacg gccgcctcta cgcgcgcggc gtgtctgatg acaaagggcc
      301 gctgctcatc ccgatcgagg tcgccgaggc gttcttcgcg acgcacgctc gcctcccgat
      361 caatgtgaag ttcatgctcg aaggcgagga ggaagtcggc agccagcacc tcgagggatt
      421 catcgcggag cacaaggatc tgctcgccgc cgacgtcgtg ctctcggccg acggcgcgat
      481 gtggcgcagc aacgagccgt cgctcaccgt cgcgaaccgc gggctggcgg ggctcgagct
      541 gacgctgcgc ggacccgcca aggacctgca ttccgggcgg cacggcggca gcgtcgccaa
      601 cccgctgcac gcgatggcgc ggctcatcgc ctcgctgcac cagccggacg gccgggtcgc
      661 cgtggcgggg ttctacgacc gggtccgcga gctcgcaccg cccgagcgcg cggagattgc
      721 agggctgccg ttcgacgagg cggcctactt ggcacagatc ggcgcgcccg cggccacggg
      781 cgagcccggc tacacgacgc tcgagcgtca gtggacccgg cccacgctcg aggtgaacgg
      841 cctgtggggc ggctaccagg ggccgggcat gaagacggtg gtgccgggcg aggcgcacgc
      901 caagatcacc tgccgcctgg tgccggacca ggatccggac gaggtggtag cgctggtgac
      961 gcgccacctg gaacgccacg tgccgccggg caccacgctg acgatccggc ccggggacca
     1021 cggcgcgcgg ccggcccgga tcgcccggga ccacccggcc ctgcaggccg cggaggccgc
     1081 cctggccgag gtgtacggcg tgcggccgct catcgtccgc atgggcggca ccgtgcccgt
     1141 cgcggagatc tttcagcgtc tcatgggact ggacaccgtg tacttctcct tttcgacggg
     1201 cgacgaggac ttccacgcgc cgaacgagtt cttccgcgtg caccgcctgc acgagggcct
     1261 ggcggcgtgg gcccgactgt gggagcggct cggccgatga cgctgcaaca ggaagaagcc
     1321 aaggtcgagg cgcacagcgc ggcgctcaag aaggtgctcg gcgtccgcga tctcgcgctc
     1381 acgcagatcc tgttcatcat cggcctcacg tggatcggcg tggccggcaa gctcgggccg
     1441 tcgcacgtgg tgttgtggct gctcgcggtc gtgctgttct acctgccgct caccggcgtg
     1501 gtcatctggc tcaaccagct gatgccgctc gagggcggac tgtaccagtg ggcgaaactc
     1561 ggcttcaacc agacgatcgg cttcatggtc gcctggaatc tgtggctgta cgtgatcctg
     1621 ttcgcctccg agctgggcct cacggtcgcg acctacctgt cgtacgcgct agggcccagt
     1681 gcggcgtgga tgacgaccag cgcatggttc gtatcattgg cgtccgcact cgctaccgcg
     1741 ctgctcgcgt tgctggcctt cgtcggcctg tcgatcggca agtgggtcca gaacgccggt
     1801 ggcgtgttca tgatcacgat cttctcggtc ctgatcctgc tgccgttcct cggcctcgcg
     1861 accggtcatc tggagtctta ccacccgttc cgcaccgaga tacccgcgtt ctcgctgttc
     1921 aatctcaacg tgctcggcaa gatgtgcgtc ggcgcgtttg ccggcttcga gtacatggcc
     1981 atcctcgccg gcgagacgca cgcgccggcg agcacggtga agaagtccgt gtactatgcc
     2041 gcgccggtca tcaccctctt tttcatcctc ggcacatcga cggtggtggc ctacatcccg
     2101 agagacgaca tcgacctcat cggcccgctg ccgcaggtcc tgcgcgtggg cttcggccca
     2161 ttcggcatcg ccgggactct ggtcacgata gcaatcgtca tgacgctggt gatgcgcgtg
     2221 tcgcagatca gcgtcgcgtt caccgccgtg acccggcttc ccatggtcgc gggctgggac
     2281 cgcctgctgc ccgcgtggtt cagccggctg cacgcgacgc accggacgcc cgtcaactcg
     2341 atcctggtgg tggccgtatt ctcatttgcg atgtcggtga tcagcct
//
LOCUS       DBNGZW010000517         7963 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_185470, whole genome shotgun sequence.
ACCESSION   DBNGZW010000517 DBNGZW010000000
VERSION     DBNGZW010000517.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7963)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7963)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7963
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_185470"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="AC16JC_12220"
     CDS             complement(<1..443)
                     /locus_tag="AC16JC_12220"
                     /inference="COORDINATES: protein motif:HMM:NF019127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HIR5165505.1"
                     /translation="MHRSSLWGLILIVTPLTARAQLPAVWDTVGRVLQATPTPLAGYV
                     RFNLPRRDLTLRIGDVTVSPRLALGAWAGFAGTSESALVMGDLVVTEAELLPVEAAID
                     SQHLAITAVHNHLVGESPRIMYVHFHGEGPAVELARRLDQVLARTA"
     gene            complement(539..1954)
                     /locus_tag="AC16JC_12225"
     CDS             complement(539..1954)
                     /locus_tag="AC16JC_12225"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR5165506.1"
                     /translation="MTPEEFRRHGHRVVDWIADYQTRVATLPVLAQVAPGAVRGRLPA
                     HPPDAPEPFAAVLRDLDEVILPGITHWQHPSFFAYFPANRSLASALGDFLSTGLGVIG
                     LNWQASPALTELELLTADWMRQMVGLSGAWSGAIHDTASTATLVALLCARERVGAFSL
                     RGGGLQSVPRPLVVYASAESHSSVEKAALLAGFGRDHLRAIPVDEAFALRPEALRDAL
                     EADRAQGLLPCAIVATTGTTGCTALDPLEPIAALAREFGCWLHVDAAMAGAAMILPEC
                     RWMWRGIEAADSIVVNAHKWLGAVFDCSLYYVRDPEHLVRVMSSNPSYLRTAADGQTV
                     NLRDWNIPLGRRFRALKLWALIRAEGVAGLQARLRRDLANAQWLAAQVDAAPHWERVA
                     PVPLQTVCVRHHPRGTDGEALDHHTLDWLERVNRSGAAFLNAAVLAGRWAVRVSIGAE
                     TTERAHVEALWQRMRDATDGV"
     gene            complement(1951..2424)
                     /locus_tag="AC16JC_12230"
     CDS             complement(1951..2424)
                     /locus_tag="AC16JC_12230"
                     /inference="COORDINATES: protein motif:HMM:NF016840.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5165507.1"
                     /translation="MINALLTAELDPRGRGAWHGPTVKGALRGVTAAQARWQPAPRRH
                     SIWELVLHIAYWNYAVRRRLEPDGPPFPRSPANWPALPSRPDARAWAKDRALLAAEHA
                     ALVRAIKRFAAGRWNRTPPAGTRWTYGEMLVGIVAHDCYHGGQISLLKRLWRSRR"
     gene            2535..3404
                     /locus_tag="AC16JC_12235"
     CDS             2535..3404
                     /locus_tag="AC16JC_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR5165508.1"
                     /translation="MVRESKVHALPGGVMILVLLVLSGLTVWRLVTAALAGSAWPIGG
                     WTLALGFVTALWFGFFVVQPNYAQVLQLFGRYVGSAKDAGLWWTNPLFTKRRVSLRVR
                     NFETAKLKVNDHNSNPIEIAAVVVWQVVDTAEAVFEVDDYDNYVHVQSESALRGLANQ
                     YPYDAHEEGQMALASHTAAIAEKLKGEIQERLAKAGVRVLEARISHLAYAPEIASAML
                     RRQQASAVIAARQKIVDGAVGMVEQALQQLSQKGIVRLDEERKAAMVSNLLVVLCSEQ
                     ATQPVVNTGTLYH"
     gene            3415..3600
                     /locus_tag="AC16JC_12240"
     CDS             3415..3600
                     /locus_tag="AC16JC_12240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin"
                     /protein_id="HIR5165509.1"
                     /translation="MAERRPFLLRIDAGLYDTLQRWAGDELRSLNGQIEFLLRRAAVE
                     AGRLREEGTRDESHRKP"
     gene            3578..5188
                     /locus_tag="AC16JC_12245"
     CDS             3578..5188
                     /locus_tag="AC16JC_12245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF020416.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5165510.1"
                     /translation="MNRIASRRRLVPALMVLATAGASLRAQQPAPFTGLYRMFQAGAE
                     VGREQFRRSPGLAQMSVVVPLLGLKLDSRTEFDSTGNFRRFEAQVYNAAGDSLRGSYT
                     VTADGDTLRSVRTTPGGETRVALPGRVAGVIPAQSVAVIALLGRRFSRDTVIRMLPMG
                     GDSTLPVTIVHRGDTAVVTLAGIEARTVIAAGRPGTIEVPMQRVRADLWDGRDSLPPL
                     AGLRRPVPDYRAPAGAPYTATEVVIPIHPAAGDSFSLAGTLTLPAAARGPVPVVVTIT
                     GSGRQTRDEDLWPLLPAYRPFREIAERLGAEGIGVLRLDDRGVGGSGGGEGTTADYAD
                     DVRQVVAWLRHRPGVAARRLALLGHSEGGAIAPLVAAQDSGLAGIVLLAGPGKTGRAI
                     LVDQLRRPIETAPGLSDSGRAAQLAGVPQAVEQIVQSSRWMRWFGDYDPLATARRVTV
                     PVLILQGALDRQVSAGQADTLAAALRAAGNRDVTEKVYPGLNHLFLRSAGDGSPSEYP
                     TLPDTKLPAQVLDDLAVWLRMKLTVPGH"
     gene            5280..7955
                     /locus_tag="AC16JC_12250"
     CDS             5280..7955
                     /locus_tag="AC16JC_12250"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HIR5165511.1"
                     /translation="MAVLIVAPPLAAQNVHPTAPPDARAVALQEPIHLDGKLDEAVWH
                     TAPAATGFRQNQPNEGQPATQRTEVRFAYDDAAIYVGARMYEDSGARGVRTRLVRRDA
                     ETDADYFQVIFDTYHDHIGRLFFTANPSGVRADANGLGGGGDDSWDPVWEVKTNIDSL
                     GWTAEMRIPFSQLRYPTTQAEQTWGLQMWRQDTRLNELSQWAFWHLNESGGPSRFGHL
                     TGMVIHRAPGKAEVLPYVVGRSSNVPVSDPTDPFQKAHAMDGRVGGDARFLLTSNLTL
                     AATVNPDFGQVEVDPAVVNLSAFETFFQEKRPFFVEGSSYFGFGGLSCFFCSNVSNLS
                     MFYTRRIGRAPQLPGNAYMTGGDYAKIPDNSTILGAAKLTGRTPTGWSIGGLDAVTGR
                     AQATVQMPGGARTRRTVEPFTNYFVARVAKDLRGGATVIRAMATSVYRNLDDPFVADS
                     LSRHAESFGLSTDMWFHRRDYHLMAQVAGTNVSGDSSAILRLQRSSARYFQRPDRGGG
                     SSGLLSERYDPSLTSLRGLGAYARFAKDQGNLLWEVSTNLRTPGFENNDIAFLSRADY
                     WWMSGNIHRMWTTPTAWYRQLFLIVGGQQQYNFDWDLTQRQGQLWGQIVLHNYWNVSG
                     FWIARPSVLDDGLTRGGPVLRRPAAHFWEGNLSTDQRKSVVVNAGGSYSRSPETSPSW
                     STSLTLQLRPASNLTLSLGPGFSHDERSDQFVRKVTDPTATTFAGVRYVFSDLRQNTI
                     SMDTRFNVTFTPNLTLELFVQPLIASGRYSRYKQFAAPRSLAKQVYGEDVGTVSVVPR
                     TGQPDSIVIDPDGGGPAQAFTLSDPSFVFRALRGNAVLRWEYRPGSTLFLVWTRSSES
                     ELSRGTISFGPDAQALFRGPAQNIFLLKMNYWLGF"
BASE COUNT         1229 a         2709 c         2835 g         1190 t
ORIGIN      
        1 gcggtgcgcg ccagcacctg gtccagccgg cgcgccagct ccacggccgg tccctcgccg
       61 tggaagtgga cgtacatgat ccgcggcgac tcccccacca ggtggttgtg taccgccgtg
      121 atcgcgaggt gctgggaatc gatggcggct tcgaccggaa gcagctcggc ttccgtgacc
      181 actagatccc ccatcaccag cgcgctctcg gacgttcccg cgaacccggc ccacgcgccc
      241 agcgcgaggc gtggggacac ggtcacgtcc cctatgcgca gcgtgaggtc gcggcgcggc
      301 aggttgaagc ggacgtatcc ggcgaggggg gtcggggtgg cctgcaggac gcggcccacc
      361 gtatcccaca cggcgggcag ctgcgcccgg gcggtgagcg gcgtgacaat caggatcagc
      421 ccccagaggg acgatcgatg cataggctca ggctcctcgt tgaggattcc aggtgtgcgt
      481 ctcgtgcccg cgcagcgcca tcatatctgc ccttgagagc ggcacagcgg gccacgcgtc
      541 acacgccgtc ggtggcgtcg cgcatccgct gccacagcgc ctcgacgtgc gcccgctccg
      601 tcgtttctgc gccgatcgac acgcgcactg cccagcggcc cgccagaacc gccgcgttga
      661 ggaacgcggc tcccgaccgg ttcacgcgct ccagccaatc tagcgtatga tggtcgagcg
      721 cctcgccgtc cgttccccgg ggatgatgtc gcacgcatac ggtctgcagc gggactggcg
      781 cgacgcgctc ccagtgcggc gccgcatcca cctgggccgc gagccactgc gcgttggcga
      841 gatcgcgtcg cagccgggcc tgcagcccag ccacgccctc ggcgcgaatc agggcccaga
      901 gcttcagcgc ccggaagcga cgccccagtg gaatgttcca gtcccgcagg ttgaccgtct
      961 ggccatcggc cgcggtgcgc aggtagctcg ggttgctgga catcacgcgc accaggtgct
     1021 cgggatcgcg cacgtagtag agcgagcagt cgaacacggc gcccagccac ttgtgggcgt
     1081 tcaccacgat cgagtcggcc gcctcgatgc cgcgccacat ccagcggcat tcgggcagga
     1141 tcatggctgc gccggccatc gcggcgtcca catgcagcca gcagccgaac tcgcgtgcca
     1201 gcgccgcgat cggctcgagc gggtcgagcg ccgtgcagcc ggtcgtcccg gtggtggcga
     1261 cgatggcaca cggcaggagc ccctgcgccc ggtcggcctc gagggcgtcg cgtaacgcct
     1321 cgggccggag cgcgaacgcc tcgtccaccg ggatggcacg caggtggtcg cggccgaatc
     1381 ccgccagcag cgccgccttc tccaccgagc tgtgactctc cgccgacgcg tacacgacga
     1441 gtggccgcgg aacggactgc aggccaccgc cgcgcaggct gaacgcgccg acgcgctcgc
     1501 gggcgcacag cagggcgacc agcgtggcgg tcgaggcggt gtcgtggatc gcgccgctcc
     1561 aggcgccgga gagccccacc atctgccgca tccagtcggc ggtgagcagc tccagctccg
     1621 tgagcgcggg agacgcctgc cagttgaggc cgatcacgcc gaggccggtc gacaggaaat
     1681 cgcccagcgc gctcgcgagc gagcggttgg cagggaagta ggcgaagaac gaagggtgct
     1741 gccagtgcgt gatgccgggc aggatcacct cgtccaggtc gcgcagcacg gctgcgaacg
     1801 gctcgggggc gtcgggtggg tgggcgggga gccggccgcg caccgctccc ggcgcgacct
     1861 gcgcgagcac cgggagcgtg gccacgcgcg tctggtaatc ggcgatccag tccacgaccc
     1921 gatggccgtg gcggcggaat tcctcgggcg tcatcggcgg ctgcgccaca ggcgcttcag
     1981 gagggagatc tgtccgccat ggtagcagtc gtgagccacg atcccgacga gcatctcgcc
     2041 gtacgtccag cgcgttcccg cgggaggggt ccggttccag cggccggccg caaaccgctt
     2101 gatcgcccgc accagcgccg cgtgctcggc agcgagcagc gcgcggtcct tcgcccaggc
     2161 ccgcgcatcg ggacgcgaag ggagcgcggg ccagttggcg ggcgagcgcg gaaacggagg
     2221 cccatccggc tcgaggcggc ggcgcaccgc gtaattccag taggcgatgt gcagcaccaa
     2281 ctcccagatg ctgtggcgtc gcggggcggg ttgccaccgc gcctgggccg cggtcacgcc
     2341 gcgcagtgca ccctttacgg tcgggccgtg ccacgcccca cgcccgcgcg gatcgagctc
     2401 ggcggtcagc agcgcattga tcatggctcg aacctgacgg cgttctcatt ggtgggcaag
     2461 gggttaggac gacttgcgca ggcgatatcc tgatgatatc atattgctag caacctacca
     2521 gcgagggtat tcccgtggtc cgcgagtcga aggttcacgc cctgcccggc ggtgtcatga
     2581 ttctcgtgct gctcgtcctg tcgggcctga ccgtctggcg gctcgtgacc gcggcgctgg
     2641 caggctccgc ctggcccatc ggggggtgga cgctggcgct ggggttcgtg acggcgctgt
     2701 ggttcgggtt cttcgtggtc cagccgaact acgcgcaggt gttgcagcta ttcggccgct
     2761 acgtaggcag cgcgaaggat gccggcctgt ggtggacgaa cccgctcttc accaagcggc
     2821 gggtgtcgct gcgggtgcgc aacttcgaga cggccaagtt gaaggtcaac gatcacaact
     2881 ccaatccgat cgagatcgcc gcggtggtgg tctggcaggt ggtggacacg gccgaggcgg
     2941 tgttcgaggt ggacgactac gacaactacg tgcacgtgca gagcgaatcg gcgctgcgcg
     3001 ggctggcgaa ccagtatccc tacgacgccc acgaagaagg gcagatggcg ctcgccagcc
     3061 ataccgccgc gatcgcggaa aagctcaagg gcgagattca ggagcggctc gccaaggccg
     3121 gcgtgcgcgt gctcgaggcg cgcatcagcc acctcgcgta tgctccggag atcgcttcgg
     3181 cgatgctgcg gcgccagcag gcgagcgccg tcatcgccgc gcggcagaag atcgtggacg
     3241 gcgccgtggg gatggtggag caggccctgc agcagctgtc gcagaagggc atcgtgcgcc
     3301 tggatgagga gcgcaaagcc gccatggtga gcaacctgct ggtcgtgctg tgctccgaac
     3361 aggcgacgca accggtggtg aacaccggca ccctgtacca ctaggaaacg gcaagtggcg
     3421 gagcgtcgcc cgttcctgct ccggatcgat gcggggctgt acgacacgct gcagcgctgg
     3481 gcgggcgacg agctgcgcag tctcaacgga cagatcgaat tcctgctgcg acgcgccgcc
     3541 gtagaggcgg gccgactgcg ggaagagggg acccgggatg aatcgcatcg caagccgtag
     3601 acgactcgtg cccgcactca tggtgctggc gactgccggc gcgtcgctcc gtgcgcagca
     3661 gccggcgccg ttcacggggc tgtaccggat gttccaggcc ggagccgaag tgggacgcga
     3721 gcagttccgt cgctccccgg ggctcgcgca gatgtcggtc gtcgtgccgc tgctcggcct
     3781 gaagctcgac agccggaccg agttcgattc cacgggcaac ttccggcgct tcgaggccca
     3841 ggtctacaat gccgcgggcg acagtctgcg cggctcctac acggtcacgg cggacggtga
     3901 cacgctgcgc agcgtgcgca cgacgccggg cggcgagacc cgtgtcgcgc tgccgggccg
     3961 ggtcgccggc gtcatcccgg cccaatccgt ggcggtgatc gcgctgctgg gccggcgctt
     4021 cagtcgcgac acggtgattc gcatgctgcc gatgggcggc gattccaccc tccccgtcac
     4081 catcgtgcac cgtggagata ccgcggtggt gacgctggcc ggcatcgagg cccgcacggt
     4141 gatcgccgcc ggccgaccgg ggacgatcga ggtgccgatg cagcgggtgc gggccgacct
     4201 gtgggacggg cgcgattcgc tgcccccgct ggccggcctg cggcgcccgg tgccggacta
     4261 tcgggcgccg gccggcgcac cgtacacggc gaccgaggtg gtgatcccga ttcacccggc
     4321 ggcgggagac agcttctcgc tcgcggggac gctcaccctc cccgccgccg ctcgcggacc
     4381 ggtccccgta gtggtgacga tcaccgggtc gggccggcag acgcgcgacg aagacctgtg
     4441 gccgctgctg cccgcgtatc gaccgttccg cgagatcgcc gaacggctgg gcgcggaggg
     4501 gatcggcgtg ctgcgactgg acgaccgggg cgtcgggggc tcgggcggcg gcgaggggac
     4561 cacggccgac tacgcggacg acgtgcgaca ggtggtagcg tggctgcggc accgtcccgg
     4621 tgtggcggcc cggcggcttg cgctgctggg ccacagcgag ggcggggcga tcgcgccgct
     4681 ggtcgcggcg caggactccg ggctcgccgg gatcgtgctg ctcgccgggc cggggaagac
     4741 cggacgggcg atcctcgtcg accagcttcg ccgccccatc gaaaccgccc cggggctctc
     4801 cgactcgggc cgggccgccc agctggcggg tgttccgcag gcggtcgagc agatcgtcca
     4861 gtcgagccgg tggatgcggt ggttcggcga ctatgacccg ctcgcgacgg cgcgccgcgt
     4921 gacggtcccg gtgctgatcc tgcagggcgc gctggaccgg caggtctcgg cggggcaggc
     4981 cgacacgctc gccgccgcgc tgcgggcggc cggcaaccgc gacgtcacgg aaaaggtgta
     5041 cccgggtctg aaccatctct tcctgcgcag cgccggagac ggctcgccga gcgagtatcc
     5101 gacgttgccg gacacgaagc taccggctca ggtgctcgac gacctcgcgg tgtggctcag
     5161 gatgaaactc acagttcccg gacactagcc gggcagcaac agcaaccttt cgcacgaccc
     5221 cggcgtcata ctcggtaatc ccgacccggg gccatgccat gtcgaagggg atcgtccgta
     5281 tggcggtgtt gatcgtcgct ccgccgctgg ccgcgcagaa tgtccacccc accgcccccc
     5341 cggatgcccg agccgtcgca ctccaggagc cgattcacct ggacgggaag ctcgacgagg
     5401 ccgtgtggca cacggctccg gcggcgacgg gatttcggca gaaccagccc aacgaaggac
     5461 agcccgcaac acagcgcacc gaagtccgct tcgcctatga cgacgccgcg atctacgtcg
     5521 gcgcccggat gtacgaggat tccggcgcgc gcggggtgcg gacccgcctg gtgcggcgcg
     5581 acgctgagac cgacgcggac tacttccagg tcatcttcga cacctatcac gatcacatcg
     5641 gccggctgtt cttcaccgcc aacccgtcgg gcgtgcgggc ggatgccaac ggcctgggcg
     5701 gcggcgggga cgattcgtgg gatccggtct gggaagtgaa gacgaatatc gactcgctcg
     5761 gttggaccgc cgagatgcga attccgttct cgcagctgcg ctacccgacg acgcaggccg
     5821 agcagacctg gggtctgcag atgtggcggc aggacacgcg gctcaacgag ctatcgcagt
     5881 gggcgttctg gcatctgaac gagagcggcg gcccgtctcg cttcggccat ctcacgggaa
     5941 tggtgatcca tcgggcgccg ggcaaagccg aggtgctgcc gtacgtggtg ggccggtcgt
     6001 ccaacgtgcc cgtgagcgat ccgaccgatc cgttccagaa ggcgcacgcg atggatggcc
     6061 gtgtcggggg ggacgcgcgc ttcctgctga cgtcgaacct gacgctggcc gccacggtca
     6121 acccggattt tggccaggtg gaagtcgacc ccgcggtcgt gaacctctcc gcgttcgaga
     6181 cgtttttcca ggagaagcgg ccgttcttcg tcgagggatc gagctacttc ggcttcgggg
     6241 gactgagctg cttcttctgc tccaacgtct cgaatttgtc gatgttctac acacggcgca
     6301 tcggccgggc gccgcagctg cccggcaacg cgtacatgac gggcggagac tacgcgaaga
     6361 tccccgacaa cagcacgatc ctcggggccg cgaagctcac gggccggacg cccacggggt
     6421 ggtccatcgg cgggctcgac gcggtgaccg gccgcgcgca ggcgacggtc caaatgcccg
     6481 ggggtgcgcg gacccggcgg accgtcgagc cgttcacgaa ctacttcgtg gcgcgcgtgg
     6541 ccaaggatct gcgcggcggg gcgaccgtga tccgcgccat ggcgacctcc gtctaccgca
     6601 acctcgacga tcctttcgtc gccgactccc tgagccggca tgcggaatca ttcggcctgt
     6661 ccaccgatat gtggttccac cgccgggact accacctgat ggcgcaggtc gcggggacga
     6721 acgtgtccgg cgattcgtcc gccatcctgc gtctccagcg atccagcgcc cggtacttcc
     6781 agcggcccga ccgtggcggc gggtcgagcg gattgctgag cgaacgctac gacccgtcgc
     6841 tcacctcgct gcgcggcctc ggggcgtacg cccggttcgc caaagaccag ggaaacctgt
     6901 tgtgggaggt gtccaccaac ctgcggacgc cgggcttcga gaacaacgac atcgccttcc
     6961 tgtcgcgggc cgactactgg tggatgagcg gcaacatcca ccggatgtgg accacgccga
     7021 ccgcctggta ccgccagctg tttctcatcg tgggcgggca acagcaatac aacttcgact
     7081 gggacctgac ccagcggcag ggacagctgt ggggccagat cgtgctgcac aactactgga
     7141 acgtcagtgg gttctggatc gcgcggccga gcgtgctcga cgacggcctg acccgcggcg
     7201 gacccgtgct gcgccggccg gcggcgcact tctgggaggg aaacctgtcg accgatcagc
     7261 ggaagtcggt ggtggtcaat gcgggcggca gctacagccg gtcgcccgag acgagcccca
     7321 gctggtcgac cagtctcacg ctgcaactgc gtccggcctc gaacctcacg ctgtcactgg
     7381 ggcccggctt ctcgcacgac gagaggagcg accagttcgt gcggaaggtc accgacccca
     7441 ccgcgacgac gttcgccggc gtccgctacg tcttctccga cctgcggcag aacacgattt
     7501 ccatggatac gcgcttcaac gtcaccttca cgcccaacct gaccctggag ctgttcgtgc
     7561 agccgctgat cgcgagcggg cggtactcgc gctacaagca gttcgcggcg ccgcgcagcc
     7621 tcgcgaaaca ggtgtacgga gaggacgtgg ggaccgtgtc ggtcgtgccg cggacgggcc
     7681 aaccggactc gatcgtcatc gatcccgacg gaggcgggcc ggcccaggcg ttcacgctct
     7741 ccgacccgag tttcgtgttc cgcgcgttgc gcggcaacgc cgtgctgcgc tgggagtacc
     7801 ggcccggctc gacgctgttc ctggtgtgga cccgcagcag cgagtccgag ctcagccgcg
     7861 ggacgatcag ttttgggccg gacgcgcagg cgctgttccg ggggccggcc cagaacatct
     7921 ttctgctgaa aatgaactac tggctgggtt tctagtcctt ccg
//
LOCUS       DBNGZW010000518        12267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_996644, whole genome shotgun sequence.
ACCESSION   DBNGZW010000518 DBNGZW010000000
VERSION     DBNGZW010000518.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 12267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12267
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_996644"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="AC16JC_12255"
     CDS             complement(<1..550)
                     /locus_tag="AC16JC_12255"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR5165512.1"
                     /translation="MNSAPQKPPELERSLGLGRATAMVVGTIIGASIFVQPSLVTGAV
                     GSIGAVFLAWGIAGVLTLAGALITAELASAYPETGGVYAFLRRAWSPGVGFLWAWAMF
                     WSMHTGIIAALSMVFARYVGYFVPLSDSGLRTVAIAVILVLSLVNVAGVRLGSALQTA
                     VTVAKVGAILILIVAGFWLGGRV"
     gene            670..1056
                     /locus_tag="AC16JC_12260"
     CDS             670..1056
                     /locus_tag="AC16JC_12260"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR5165513.1"
                     /translation="MRNIWVTLDWVARIGTAILFIKAGISHIRKRRAMVAYVQSKHLP
                     LAWLDVVGTGILMLVGSALLASNWHPHWGAALIILFLVPTAVLMHNYWAESAPMARSN
                     QDAHFWKNITIAFALLLYIVQGHLHG"
     gene            complement(1060..1773)
                     /locus_tag="AC16JC_12265"
     CDS             complement(1060..1773)
                     /locus_tag="AC16JC_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165514.1"
                     /translation="MRSRSLGLLLGTLALSVVACRGREGGDRGKTASAVPESTVSAPT
                     PPPPPPPRSLPSPFPADKDTARLQTRLAGPAAGVPSCGSGVPRITADSIGPFRPGTTV
                     SELARRCPGLLHGWVMISDGFPVPTVAARVGGARVTGFASDSSSDAIVNRIELVGAGP
                     RTAEGLGVGSTLGQLQSVYGAPQASESDCVLMVWFDTRPGLNFRLEYPPRAPRDCGAL
                     SEPPLPPDLRVASVVIVQR"
     gene            complement(1876..3150)
                     /locus_tag="AC16JC_12270"
     CDS             complement(1876..3150)
                     /locus_tag="AC16JC_12270"
                     /inference="COORDINATES: protein motif:HMM:NF047558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5165515.1"
                     /translation="MSASLAILPLANASGDPDKDYFADGLTECLHSALVGVRALRVAS
                     RGAAAAHHGQVARADLARALDVAAVLEGSVTRLGGQLRLSAGLHAADGGTHWSVQMDE
                     KLDELFPVLHRLGERILTTLGAAPTPQEQQALEYVPTREPRALDPYLRARHLSGQIRR
                     SSQDLAVQLYGDAAQADPGFALAHAGLAESHALLFTYWDSIEDHLRTADSASDRAVAL
                     APMLAETHLARGLALSLSRRHDAAEAEFLAALDLKPNLFEAHYHYARHCRTTGRLEES
                     VRWFESAGRLRPEDYATPALLASVYVSLQRTTESEAAQRRAVVLAEQRLKLVPDDERA
                     LYLGAGCLSSLGDRSRAREWAKRAVAMEPDDSAVLYNVACVYALLGLADSAIDCLERS
                     LANGFGHWEWIEHDSDLDPLRAHPRFQALRRT"
     gene            complement(3147..4121)
                     /locus_tag="AC16JC_12275"
     CDS             complement(3147..4121)
                     /locus_tag="AC16JC_12275"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HIR5165516.1"
                     /translation="MLRRCILSRPSGRLARQAQADIGTEASRILLIEDNPRHAQLLRE
                     ALGEVVPAVSGLVPYDLTHVPGLAEGLELLGSQEFDVVLLDLSLPEAPGLDALVRIRE
                     RHEDVPVIALTARGEDDLAVQAIQAGAQDFLQKGRISGELLTRAIRAAVHISNLQAAL
                     RSLSFIDGLTGLYNRRGFVMLGDPYIKRAQRQKGQFLIVSADISGLKQINTIASYDEG
                     DAVLRAVAEILKRSFRDSDLLARIEGGTFAVMAVDANADKANIIASRVQYNVEEWNNR
                     TIRRYQLKVNLGFTEFDPTGAIEDLIARAADSRPSRRRRTGPGKKRSE"
     gene            4158..4349
                     /locus_tag="AC16JC_12280"
     CDS             4158..4349
                     /locus_tag="AC16JC_12280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165517.1"
                     /translation="MTVPRQSPRPLRTLVLGWGCSPGAATPLRRIAGVPDTRRFVIEI
                     EQGWDLIGAPTNLWLYELP"
     gene            complement(4346..4885)
                     /locus_tag="AC16JC_12285"
     CDS             complement(4346..4885)
                     /locus_tag="AC16JC_12285"
                     /inference="COORDINATES: protein motif:HMM:NF014664.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR5165518.1"
                     /translation="MSPRSRHLPFPDRVMGWIGAVPRRDPEPLPGPCGIAAYARAGWA
                     RSSPVALEPRLRLLVTQVAAVRSECAYCVQYNRHVGLRGGIPAATLDAVVDYARSPHF
                     SHLERAALALADALTRFAAAQGGFAAEILERARCHLAEEQIMDLVTVVATEHFFDPIT
                     GALGRDARAASLQDDRPNH"
     gene            complement(4882..5802)
                     /locus_tag="AC16JC_12290"
     CDS             complement(4882..5802)
                     /locus_tag="AC16JC_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromate resistance protein ChrB
                     domain-containing protein"
                     /protein_id="HIR5165519.1"
                     /translation="MADSIVSPQRWLLLIHQLPPKPDYPRVKLRRRLQRLGAVPLKRT
                     VYALPAGDQAREDFEWLAREIAADGGEATLCESTFLDPDTDRRLVQATRSACTSGYEA
                     IAAASHGGAADLDRLTRRLHEVQALDHFAADGRADAEAALEALTTRASQPGALMMPTA
                     LPDKPKARVWVTRAGVYVDRIASAWLIRRFVDAAAQFRFVAPKQYHPEPGELRFDMFE
                     GEFTHDGDSCTFETLCRHFAIRDPALKAIGEIVHDIDCKDDKFGREETPGLQLVLDAL
                     TRAHAADEDRLTRGGALFDELSAQLRKGRP"
     gene            5920..6564
                     /locus_tag="AC16JC_12295"
     CDS             5920..6564
                     /locus_tag="AC16JC_12295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165520.1"
                     /translation="MRRSLLLAGLGLLAVPLAAQEFNQFCRGNPKLTVGQWSSYRYVG
                     GPADGSTMRMAIVGSERQGDSTFLWYEMNIVDAKHADRGPTITQILVSGLGTPDLSIH
                     GMVMKAGKNKAMRYPDMMVGMMSGPIKKGVGSLVEQKCKKGAVQEIGWESVTVPGGTF
                     RALHFKDGDDEAWLSPELGFTLVRYVSKKNGTMELTGHGTDAKSSITETPQTMD"
     gene            6564..7136
                     /locus_tag="AC16JC_12300"
     CDS             6564..7136
                     /locus_tag="AC16JC_12300"
                     /EC_number="1.1.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008873 - gluconate 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0008875 - gluconate dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HIR5165521.1"
                     /translation="MDRRTALRLLSAGAAVTVGTDFAAWGRALHRGLGRGAPLRVLDP
                     HQNATVVALCDTILPATDTPGAVAAQVNEFIDLLLADWYDAPERDRFLTGLGGLDGRA
                     RAAFGKDFVDGTVADRTNLLTALDDEAARWNASPEATRGPQPFYRQMKWLTLFGYYTS
                     EIGGEQEQHYQIVPGRYVPCAPADTTIAPE"
     gene            7139..8821
                     /locus_tag="AC16JC_12305"
     CDS             7139..8821
                     /locus_tag="AC16JC_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020531782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC oxidoreductase"
                     /protein_id="HIR5165522.1"
                     /translation="MPKTWDAIVIGSGMTGGWAAKELTEAGLNTLVLEAGRMVTAADY
                     VEHVPPWAVRFRGLDDRRARAADQPIQRECYACDEWSSKFFVNDHENPYTTPADKPFL
                     FLRGRQVGGRSIMWGRQVYRWSDLDFEANLKDGVAVDWPIRYADIAPWYDHVERFIGV
                     SGEKLGLSQLPDGQFLPPMDLNCGETVVRDAVAQHWPGERVLTIGRCAILTDDHNDRK
                     ACHYCGYCERGCITRSYFSSINATLPAARLTGRLTLRPHSVVHSLIYDRRRSRVTGVR
                     VIDGETKHALEFRGRVVFLCASTLESARILLNSATPEFPTGLANSSGELGHNLMDHIM
                     GGGANGKIDAPADRNPVGRRPNGIYLPRFRNLQGGPKSPFLRGYAYQGGAGREGWERG
                     TDLPGFGADWKRALRDPGPWHFGFYGFGECLPNHQNYVEIDKDTKDAWGIPVLRIHCA
                     YGDNEWALMKDASIAAAEMLAAAGASDITPFIDHNPPGKAIHEMGTARMGRDPKTSVL
                     NAHNQAHDIPNLFVTDGACMTSSANQNPSITYMALTARACDYAVGAMKRREI"
     gene            8821..9684
                     /locus_tag="AC16JC_12310"
     CDS             8821..9684
                     /locus_tag="AC16JC_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5165523.1"
                     /translation="MERRDFLGSLAAATVLPRFTTACLGAVTRARIAKVGLQLYTVRD
                     LMKADFEGTLAKVAQIGYREVEFAGYFDHTAEQVRAVLDKNGLASPSCHVGYGDATGP
                     GWSATLEKVKAVGHQYVVCAWIDEQARKSADDWKRICDQFNTAGTAAQAAGLQFAYHN
                     HSFEFEPLADGRKPYDVLLANTDPKLVRLEVDLFWITFGGVDPLAYFTKYPGRFPLVH
                     VKDMMPKPAPDVAPDKVMADVGKGSIDWKGIFAHSSQGGIEHYFVEHDQPADPLASIG
                     TSYRYLRDLEF"
     gene            complement(9681..>12267)
                     /locus_tag="AC16JC_12315"
     CDS             complement(9681..>12267)
                     /locus_tag="AC16JC_12315"
                     /inference="COORDINATES: protein motif:HMM:NF053442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR5165524.1"
                     /translation="YFCSDRDGTMNLWSMDPEGHDLRQLTRHRGWDVRSPALGDGRIV
                     YQLGADLHRYDIARGTDTTLDIRLGSDFDQERVRWVSNPMTYLTAAHLAPDGGRVVLT
                     ARGQVFVAPVGRAGGRFVEATRAPGVRYRQARFAPDGKSLLALSDQSGETEWWRLPAN
                     GVGTPEQVTSDARVLRWDGVPSPDGKWLASYDKNQTLWLYGFDRRQATLVDSTPDGDF
                     GDLVWSPDSKWLAYVRPAATFGRIWLYGVAQGQRTALTSDRSDSYSPAFTPDGKWLYF
                     LSDRTFQSLVGAPWGPRAPEPFFDRQTKLYALALVPGLRSPFLPATELAPPAAGDTGT
                     KPRPTAAPAVTVDLAGVQQRLTELPLPAANYEALSVSGSRLFWLSRQTNLERTAALQS
                     LEIGNSDFTPKTLVDDVRSYELAADGKQLLLRKGNALYVIDAASGAGASLDKAQVNLA
                     DWSFPLDPREEWRQMFDEAWRLERDYFYDPHMHGLDWPAVKAKYRPLVDRVTDRAELS
                     DLLAQMVSELSALHIFVYGGDLRDGTDQAAPASLGAELTRDSAAGGWRVAHVYGADPD
                     YPTTLSPLAQQGVDVHAGEVIQLINGVSTLSVEHPGELLRRQAGKQVLLRIAPARGGP
                     ARDVIVTPLTPGQASNLRYDEWELTRRTLVDSGSEGRIGYVHLRAMGPPDIARWERDF
                     YPVFNRAGLIIDVRHNRGGNIDSWLLERLLRRAWFYWQPRTGSPYWNMQWAFRGHVVV
                     LCDAWTASDGEAFTEGFRRLGLGRVIGTRTWGGEIWLSSSNVLVDRGIATAAEFGVYG
                     PEGQWLIEGHGVDPDSVVDNLPHATFGGEDAQLAAAIAYLRRQIREHPVPVPPAPAYP
                     RKGGP"
BASE COUNT         1945 a         4191 c         4294 g         1837 t
ORIGIN      
        1 gcacccgccc gcccagccag aatcccgcga cgatcaggat gaggatcgcg cccactttgg
       61 cgacggtgac cgcggtctgg agcgcgcttc ccagccggac ccccgcgacg ttcacgaggg
      121 agagcaccag gatcaccgcg atggcgacgg tgcgcaggcc cgagtcgctc aaggggacga
      181 agtagcccac ataccgcgcg aacaccatgg agagggcggc gatgatgccg gtgtgcatgc
      241 tccagaacat cgcccaggcc cagagaaacc caaccccggg cgaccaggcc cggcgcagaa
      301 acgcgtacac cccgccggtt tccggatagg ccgaggcgag ctcggccgtg atcagggcgc
      361 cggcgagcgt gagcaccccc gcaatgcccc aggcgaggaa caccgcgccg atcgacccca
      421 cggcgcccgt gacgagcgac ggctggacga agatcgacgc cccgatgatg gtgccgacga
      481 ccatcgccgt ggcgcgtccc agcccgagtg agcgctcgag ctccggtggc ttctgtggcg
      541 cggaattcat gggtccgctg gcgaccaaca tagcgcgcgg gcggggctcg gtcgccaagg
      601 tggaagaatc cgggcggcga tgtgatcgtt gtcgggtacc ttgggatgac ccacactgga
      661 ggacgcgcca tgcgcaacat ctgggtgacg ctcgactggg tggcccggat cggcaccgcg
      721 atcctgttca tcaaggcggg gatcagtcac atccgtaagc gccgggcgat ggtcgcctac
      781 gtgcagtcga agcacctgcc gctggcctgg ctggacgtcg tcgggaccgg catcctgatg
      841 ctcgtgggat cggcgctgct ggcgtcgaac tggcacccgc actggggagc cgccctgatc
      901 atcctcttcc tcgtgcccac cgcggtcctg atgcacaact actgggccga atccgccccc
      961 atggcgcgga gcaaccagga cgcgcatttc tggaagaaca tcaccatcgc gttcgccctg
     1021 ctgctctaca tcgtccaggg gcatctgcac gggtgagcgt cagcgctgca cgatgactac
     1081 ggaggcgacg cgcaggtcgg ggggcagggg tggctcgctc agggcgccgc agtcgcgcgg
     1141 cgcccgcggc ggatactcca ggcggaagtt cagcccgggg cgcgtatcga accagaccat
     1201 caggacgcaa tcggactccg acgcctgcgg cgcgccgtag acgctctgca gttggccgag
     1261 cgtcgagccg actccgagcc cttcggctgt gcgtgggccg gcgcccacca gctcgatccg
     1321 gttcacgatc gcgtcggacg aggagtcgct ggcgaagccg gtgaccctgg cgcctcccac
     1381 tcgcgccgcc accgtcggca ccggaaagcc gtcggagatc atcacccacc cgtggagcag
     1441 tcccggacag cgccgggcca gctccgagac ggtcgtcccc ggacgaaacg gcccgatcga
     1501 gtccgccgtg atccgcggca cgccgctgcc gcacgacggc acgccggccg cggggcccgc
     1561 cagcctggtt tgcagccgcg ccgtgtcctt gtccgctggg aacggcgagg ggagggatcg
     1621 cgggggaggg ggagggggag gtgtcggagc cgagaccgtg gattcgggaa cggcggaagc
     1681 cgtcttgccg cgatcgccgc cctcgcgtcc ccggcatgcc acgacgctca gcgcgagcgt
     1741 gcccagaagc agacccagcg agcgactccg catcaatgtc tccgcgtttg cgggttcacg
     1801 gcagcgagtc cggcgaggat ctcccgggcg tgcctcgcga cgttgccggc catcggtcaa
     1861 gccgccgtcc attgctcagg tccgtcgcag cgcctggaac ctcgggtgcg cgcgcagcgg
     1921 atcgaggtcc gagtcgtgct cgatccactc ccagtggccg aacccgttgg cgagcgagcg
     1981 ttccaggcaa tcgatcgcgg agtcggcgag tccgagcagg gcgtacacac acgccacgtt
     2041 gtacaggacc gcggaatcgt cgggctccat cgccaccgcg cgcttcgccc attcgcgtgc
     2101 ccggctgcgg tcgcccagcg aagagaggca gccggcgccc aggtagagcg cccgctcgtc
     2161 gtccgggacc agcttgagcc gctgctcggc gagcaccacg gcgcggcgct gcgccgcctc
     2221 ggactccgtc gtccgctgca ggctcacgta cacgctcgcc agcagcgccg gggtggcata
     2281 gtcctcggga cgcaggcgac cggccgactc gaaccagcgc acggattcct ccagacggcc
     2341 ggtggtgcgg cagtggcgcg cgtagtggta gtgcgcctcg aacaggttgg gcttgagatc
     2401 gagcgcggcc aggaattccg cttccgccgc gtcgtgccgg cggctgagcg acagcgccag
     2461 gccgcgcgcc agatgcgttt cggcgagcat cggtgccagc gcgaccgccc ggtcgctcgc
     2521 cgagtcggcg gtgcgcaggt ggtcttcgat cgagtcccag taggtgaaca ggagcgcgtg
     2581 cgactcggcg agacccgcgt gcgccagcgc gaaccccggg tcggcctggg cggcgtcgcc
     2641 gtacagctgg accgccaggt cctggctcga tcggcggatc tgaccggaga ggtgacgggc
     2701 gcgcaggtag gggtcgaggg cgcgcggctc ccgcgtcggg acgtattcca gcgcctgctg
     2761 ttcctggggt gtcggcgccg cgccgagggt ggtgaggatc cgctcgccga gtcggtgcag
     2821 cacggggaac agctcatcga gcttttcgtc catctggacc gaccagtggg tcccgccgtc
     2881 cgcggcgtgg aggccggcgc tgaggcgcag ctgtccgccc aggcgggtga cgctgccctc
     2941 gagcaccgcg gccacatcga gcgcacgcgc gaggtcggcc cgggcgacct gtccgtgatg
     3001 ggccgcggcg gctccgcggg aggccacgcg cagcgcccgg acgccgacca gtgcgctgtg
     3061 caggcactcc gtcagaccgt ccgcgaagta atccttgtcg gggtccccgc tcgcgttggc
     3121 gagcgggagg atggcgaggg acgcgctcac tccgaccgtt tcttcccggg tccggtgcgc
     3181 cggcgacgcg aggggcgcga gtcggcggcg cgagcgatga gatcctcgat cgctccggtc
     3241 ggatcgaact ccgtgaatcc gaggttcacc ttcagctggt aacggcggat cgtgcggttg
     3301 ttccactcct cgacgttgta ctggacgcgg ctggcgatga tgttcgcctt gtcggcgttc
     3361 gcgtccaccg ccatcaccgc gaacgtgccc ccttcgatcc gggccagcag gtccgagtcg
     3421 cggaagctcc gcttgagtat ctcagccacc gcgcgcagta ccgcatcgcc ctcgtcgtag
     3481 ctggcgatgg tgttgatctg cttgagcccc gagatgtccg ccgacacgat gagaaactgg
     3541 cccttctgac gctgcgcgcg cttgatgtag gggtctccca gcatgacgaa cccccggcgg
     3601 ttgtagagac cggtcaggcc gtcgatgaac gacaggctgc gcagcgccgc ctgcagattc
     3661 gagatgtgca ccgccgcgcg gatcgcgcgc gtcagcagct ccccgctgat cctccctttc
     3721 tggaggaaat cctgcgcccc ggcctggatc gcctggacgg ccaggtcgtc ctctccgcgg
     3781 gcggtgagcg cgatgacggg gacgtcctcg tgccgctccc ggatgcgcac cagggcgtcg
     3841 agcccggggg cttcggggag cgacagatcg agcagcacga cgtcgaattc ctggctgccg
     3901 agcagttcga gaccttcggc caggccgggg acgtgggtca gatcgtaggg caccagaccc
     3961 gagacggcgg gaacgacttc gcccagcgcc tcccggagca gctgggcatg ccggggattg
     4021 tcctcgatca gcaggatgcg gctcgcttca gtgccgatgt ccgcctgcgc ctgacgggcg
     4081 agacgacctg atggacggga caggatgcac ctccgaagca atgagccact tccccgatcc
     4141 gcgggaaatt tgccggtatg accgtcccgc gccagtcgcc tcgcccgctg cgcaccctgg
     4201 tgctcggctg ggggtgctcg ccgggtgcgg ccactcccct acggcgcatc gcgggagtgc
     4261 ccgacacgcg ccgcttcgtg atcgaaatcg agcagggatg ggacctgatc ggcgctccga
     4321 ccaacctctg gctgtacgag ctgccctagt ggttcggccg gtcgtcctgg agtgacgcgg
     4381 cgcgcgcgtc acggcccagg gcgccggtta tcgggtcgaa gaagtgctcc gtcgccacca
     4441 ccgtgacgag gtccatgatt tgctcctcgg ccagatggca gcgcgcccgc tccaggatct
     4501 cggcggcgaa ccctccttgc gccgccgcga atctcgtgag ggcatcggcc agcgccaggg
     4561 ccgcgcgttc gaggtgggag aagtggggcg accgcgcata gtccaccacg gcgtccaggg
     4621 tggcagcggg gatcccgccg cgcaggccga catgccgatt gtactgcacg cagtaggcgc
     4681 attcgctgcg cacggcggcg acctgggtca cgagcagccg cagccgcggc tcgagcgcga
     4741 cgggggaaga ccgggcccag cccgcgcggg cgtatgcggc gatcccgcaa ggccccggga
     4801 gcggctcggg gtcccggcgc ggcacggcgc cgatccatcc catcacgcgg tccgggaagg
     4861 gcaggtggcg gctgcgcggg ctcatggccg tcccttgcgc agctgcgcgg acaactcgtc
     4921 gaacagcgcg ccgccgcgcg tgaggcgatc ctcgtcggcc gcatgggccc gggtgagggc
     4981 gtccagcacg agctgcagtc cgggtgtctc ctcccggccg aacttgtcgt ccttgcagtc
     5041 gatgtcatgc acgatctcac cgatagcctt gagggccgga tcgcggatgg cgaagtgtcg
     5101 gcacagtgtc tcgaaggtgc acgaatcgcc gtcgtgagtg aactccccct cgaacatgtc
     5161 gaaccggagc tcgccgggct ccgggtgata ctgcttgggg gccacgaagc ggaactgcgc
     5221 cgcggcatcg acgaagcgcc ggatcagcca cgcgctcgcg atgcgatcga cgtagacgcc
     5281 ggcgcgggtg acccacacgc gggccttcgg cttatcgggg agggcggtcg gcatcatcaa
     5341 ggctccgggc tgggaggcgc gggtcgtcag tgcctcgagc gccgcctcgg cgtcggcgcg
     5401 tccgtcggcc gcaaagtgat ccagcgcttg cacttcgtgg agtcgtcgag tgaggcggtc
     5461 cagatcggcc gcaccaccgt ggctggcggc ggcgatcgcc tcgtagcccg aggtgcaggc
     5521 gcttcgagtg gcctgcacca atcggcggtc ggtatcggga tcgaggaacg tggactcaca
     5581 gagcgtcgct tcaccaccgt cggccgcgat ctcgcgcgcc agccactcga aatcctcgcg
     5641 agcctggtcg ccggccggca gcgcatagac ggtgcgtttg aggggcacgg cgccgagccg
     5701 ctggagccgg cgccggagct tgacgcgggg gtagtcgggc ttgggaggga gctggtggat
     5761 cagcagcagc cagcgctgtg gtgatactat agaatcagcc attgattcta ttgtatcatc
     5821 gatcggcacg cgccgcaagg gcttgccgac cgcccatgga caggatatct gacgtggggt
     5881 aggtcttcac cctccacaaa cgactcagga gctcacccga tgcgccgcag tctcctcctc
     5941 gccggcctcg gccttctggc cgtcccgctt gccgctcagg agttcaatca gttttgccgg
     6001 gggaacccga agctcaccgt cgggcaatgg tcgagctatc gctacgtggg tgggccggcc
     6061 gacggctcga ccatgcgcat ggccatcgtc gggtcggagc gccagggcga ctccacattc
     6121 ctctggtacg agatgaacat cgtcgatgcc aagcacgccg atcgcggacc caccattacg
     6181 caaatcctgg tcagcgggct cggcacgccg gatctctcga tccacggcat ggtgatgaag
     6241 gcagggaaga acaaggcgat gcggtatccc gacatgatgg tcgggatgat gtcggggccc
     6301 atcaagaagg gtgtggggag cctggtcgag cagaagtgca agaaaggtgc ggtccaggaa
     6361 atcggatggg agagcgtgac cgtgccggga gggacattcc gcgcattgca cttcaaggac
     6421 ggcgacgacg aagcctggct gagtcccgag ctcgggttta cgctcgtgcg gtacgtctcg
     6481 aagaagaatg gaacgatgga gctcaccggc cacggcacgg acgcgaaatc gtcgatcacc
     6541 gaaacgccgc agacgatgga ctgatggacc ggcgaacggc attgcgcctg ctgagtgccg
     6601 gggcggccgt caccgtcggc accgacttcg cggcgtgggg acgcgcgctg caccgcggcc
     6661 tgggccgtgg cgcgcccctc cgggtgctcg atccgcacca gaacgcgacc gtcgtcgccc
     6721 tgtgcgacac catcctcccg gccactgata ctcccggcgc cgtcgcggcg caggtgaacg
     6781 agttcatcga cttgttgctc gcggattggt atgatgcgcc ggagcgggac cggtttctca
     6841 ccggcctcgg cggtctcgac gggcgcgccc gggcagcgtt cggcaaggac ttcgtggacg
     6901 gcaccgtggc ggaccgcacc aacctcctga cggccctcga cgacgaggcg gcgcgctgga
     6961 acgcgagccc ggaagcgacg cgcggcccgc agccgttcta ccggcagatg aagtggctca
     7021 cgctgttcgg ctactacacg tccgaaatcg gcggcgagca ggagcagcac taccagatcg
     7081 tccccggccg ctacgtcccc tgcgcgccgg ccgacaccac catcgctccg gagtgaagat
     7141 gccgaagacc tgggatgcga tcgtgatcgg ctcggggatg accggtggct gggcggccaa
     7201 ggagctcacc gaggcggggt tgaacacgtt ggtgctcgag gcgggccgga tggtgacggc
     7261 cgccgactac gtcgagcacg tgcctccctg ggcggtgcgg ttccgcggcc tggacgaccg
     7321 gcgcgcccgg gcggcggacc agcccatcca gcgcgagtgc tacgcctgcg acgagtggtc
     7381 gtccaagttc ttcgtcaacg accacgagaa cccctacacc acgccggccg acaagccgtt
     7441 cctgttcctg cgcgggcgcc aggtgggtgg acgctccatc atgtggggcc gacaggtcta
     7501 ccgctggagc gacctcgact tcgaggcgaa cctgaaggac ggcgtcgccg tcgactggcc
     7561 gattcgctac gccgacatcg cgccgtggta cgaccacgtg gagcggttca tcggcgtgag
     7621 cggcgaaaag ctggggcttt cccagctgcc cgacggccag ttcctgcccc cgatggacct
     7681 gaattgcggc gagacggtcg tccgcgacgc cgtcgcgcag cactggccgg gcgagcgagt
     7741 gctcaccatc ggccgctgcg ccattctcac cgacgatcac aacgaccgta aagcctgcca
     7801 ctactgcggc tactgcgagc gcggctgcat tacgcggtcc tatttcagct cgatcaatgc
     7861 gacgctcccc gcggcgcgcc tgaccggcag gctcacgctg cggccgcaca gcgtcgtcca
     7921 cagtctgatc tacgaccgcc gtcgcagtcg ggtgacgggc gtgcgcgtga tcgacggcga
     7981 gacgaaacac gccctcgagt tccgcggtcg cgtggtgttt ctctgtgcct cgacgctcga
     8041 gtcggcgcgt atcctgctca actcggcgac gccggagttt cccaccgggc tggccaactc
     8101 gagcggcgag ctgggccata acctgatgga ccacatcatg ggtggcggcg cgaacggcaa
     8161 gatcgacgcg cccgccgacc ggaaccccgt aggccggcgt cccaacggga tctatctccc
     8221 gcggttccgc aaccttcagg gcggtcccaa gtcgccgttc ctgcgcggct acgcctatca
     8281 gggcggcgcg gggcgcgaag ggtgggagcg cggcaccgat ctccccggct ttggcgccga
     8341 ctggaagcgg gcgctgcggg accccgggcc gtggcacttc ggcttctacg gcttcggcga
     8401 gtgcctcccc aaccaccaga actacgtcga gatcgacaag gacaccaaag acgcctgggg
     8461 cattccggtg ctgcgcatcc attgcgccta cggcgacaac gaatgggccc tcatgaagga
     8521 cgcgtccatc gcggcggcgg agatgctggc cgccgccggt gcgagcgata tcacgccgtt
     8581 catcgatcac aacccgcccg gcaaggcgat ccacgagatg ggcaccgcgc ggatgggccg
     8641 cgatccgaag acgtcggtgt tgaacgccca caaccaggcg cacgatattc cgaatctgtt
     8701 cgtcaccgac ggcgcctgca tgacgtcctc ggcgaaccag aacccgtcga tcacctacat
     8761 ggcgctcacc gcccgggcgt gtgattacgc ggtcggggcg atgaagcgga gggagatcta
     8821 atggaacgcc gtgattttct cggatcgctc gcggccgcga cggtgctgcc ccgcttcacc
     8881 accgcgtgtc tcggtgccgt cacgcgagcc cggatcgcga aggtgggcct gcagctctac
     8941 accgtgcgcg acctcatgaa ggcggacttc gagggcacgc tcgcaaaggt ggcgcagatc
     9001 ggctaccgcg aggtggagtt cgcgggatac ttcgatcaca ccgcggagca ggtgcgggcg
     9061 gtgctcgaca agaacggcct cgcctcaccc tcgtgccatg tcggatacgg cgacgccacc
     9121 ggccctggct ggtccgctac cctcgagaaa gtcaaggccg tgggccatca gtatgtggtg
     9181 tgtgcctgga tcgacgaaca ggcacgcaag agcgcggacg attggaagcg catctgcgat
     9241 caattcaata cggcggggac agccgcacag gccgcggggc tccagttcgc ctatcacaac
     9301 cacagcttcg agtttgagcc gctcgcggat gggcggaagc cgtacgacgt cctcctcgcg
     9361 aacaccgatc ccaagctggt gcggctcgag gtcgatctct tctggatcac cttcggcggc
     9421 gtggatcccc tggcgtactt cacgaagtat cccggacggt ttcccctggt gcacgtgaag
     9481 gacatgatgc cgaagccagc gcccgacgtc gcgcccgaca aggtcatggc cgacgtaggg
     9541 aaggggagca tcgactggaa ggggatcttc gcccattcga gccagggcgg catcgagcac
     9601 tatttcgtgg agcatgacca gccggccgat cccctggcca gcatcggtac cagctaccgg
     9661 tacctgcgcg acctcgagtt ctagggcccg cccttccgcg ggtacgccgg tgccggcggg
     9721 accggcacgg ggtgctcgcg gatctggcgc cggagatacg cgatcgccgc cgcgagctgc
     9781 gcgtcctccc cgccgaacgt cgcgtgcggc aggttgtcca cgacgctgtc ggggtccacc
     9841 ccgtggccct cgatcagcca ctggccctcc gggccgtaca cgccgaactc cgcggccgtg
     9901 gcgatgccgc gatccaccag cacgttgctc gagctgagcc agatctcccc gccccacgtg
     9961 cgcgtgccga tcacccggcc caacccgagc cgccggaacc cctcggtgaa ggcctcccca
    10021 tccgacgccg tccacgcgtc gcacagcacc accacgtggc cccggaacgc ccactgcatg
    10081 ttccagtagg gactcccggt gcgcggttgc cagtagaacc aggcgcgacg cagcagccgc
    10141 tcgagcagcc agctgtcgat gttgccgccg cggttgtggc gcacgtcgat gatcaggccg
    10201 gcccggttga agacggggta gaaatcgcgc tcccagcgtg cgatatcggg cgggcccatg
    10261 gcccgcaggt gcacgtagcc gatgcgaccc tcgctgccgg agtccaccag cgtgcgccgc
    10321 gtcagctccc attcgtcgta gcgcaggttg gacgcctgcc ccggggtcag gggcgtaacg
    10381 atgacgtcgc gcgcggggcc gccgcgcgcg ggcgcgatcc gcaacagcac ctgcttcccg
    10441 gcctggcgcc ggagcagctc gcccgggtgc tccaccgaca gggtcgacac gccgttgatc
    10501 agctggatga cctccccagc gtggacgtcc acgccctgct gggcgagcgg ggagagcgtc
    10561 gtggggtaat cgggatccgc gccgtagaca tgcgccactc gccagccgcc cgccgcggag
    10621 tcgcgggtca actccgcgcc gagtgaggcg ggagccgcct ggtccgtgcc gtcgcgcagg
    10681 tcgccaccgt acacgaagat gtgtagcgcc gacagctcgc tcaccatctg cgccagcaga
    10741 tccgacagct cggcgcgatc ggtcacccgg tccaccagcg gacggtactt ggccttcacc
    10801 gcgggccagt cgagcccgtg catgtgcggg tcgtagaagt agtcgcgctc cagccgccag
    10861 gcctcgtcga acatctggcg ccactcttca cggggatcga gcgggaagct ccaatcggcg
    10921 aggttcacct gggccttgtc cagactcgca ccggcgccgc tcgctgcgtc gatcacgtac
    10981 agcgcgttgc ccttgcggag caggagctgc ttgccgtccg ccgccagctc gtagctgcgc
    11041 acgtcgtcca ccagcgtctt cggcgtgaag tcgctgttgc cgatctcgag cgactggagt
    11101 gccgccgtgc gctccaggtt ggtctggcgg ctcagccaga acaggcgact cccgctcacc
    11161 gacagcgcct cgtaattggc ggccggaagc ggcagctccg tcaggcgctg ttgcacaccg
    11221 gccaggtcca cggttaccgc cggcgcggcg gtggggcgcg gtttggtgcc ggtgtcgccg
    11281 gcggcggggg gcgccagttc ggtcgccggc aggaacggcg agcgcagtcc cggcacgagc
    11341 gccagtgcgt acagcttcgt ctgccggtcg aagaaaggct cgggcgcgcg cgggccccac
    11401 ggggccccga cgagtgactg gaacgtccgg tccgacagga agtacagcca cttcccgtcc
    11461 ggcgtgaacg ccggactgta gctgtcggag cgatcgctgg tcagcgcggt gcgttggccc
    11521 tgggcgacgc cgtagagcca gatccggccg aaggtcgcgg cgggacggac gtaggcgagc
    11581 cacttcgaat cgggggacca gacgaggtcg ccgaaatccc cgtcgggggt cgagtcgacg
    11641 agggtcgcct gccggcggtc gaacccgtag agccacaggg tctggttctt gtcgtaggag
    11701 gcgagccact tgccgtccgg cgaggggacg ccgtcccagc gcagcacccg cgcgtcgctc
    11761 gtgacctgtt ccggggtgcc cacgccgttg gcgggcaggc gccaccactc cgtctccccc
    11821 gactgatcgg acagcgcgag caaggacttt ccatccggtg cgaagcgtgc ctggcggtac
    11881 cgcactcccg gggcccgcgt cgcctcgacg aagcggcccc cggcccgccc gacgggcgcc
    11941 acgaacacct ggcctcgcgc ggtgagcacc acgcgcccgc cgtcgggagc gaggtgcgcg
    12001 gcggtgaggt acgtcatcgg attggacacc cagcgcaccc gctcctggtc gaagtcggag
    12061 ccgaggcgga tgtcgagcgt cgtatcggtg ccacgggcaa tgtcgtagcg gtgcaggtcg
    12121 gcgccgagct ggtacacgat ccgtccgtcg cccagcgcgg gcgaccgcac gtcccagcca
    12181 cgatggcggg tcagctggcg caggtcgtga ccctcgggat ccatggacca caggttcatc
    12241 gtgccgtcgc ggtcggagca gaagtag
//
LOCUS       DBNGZW010000519         6921 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_594349, whole genome shotgun sequence.
ACCESSION   DBNGZW010000519 DBNGZW010000000
VERSION     DBNGZW010000519.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6921)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_594349"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="AC16JC_12320"
     CDS             complement(<1..320)
                     /locus_tag="AC16JC_12320"
                     /inference="COORDINATES: protein motif:HMM:NF014010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:2000185 - regulation of phosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex PhoU family protein"
                     /protein_id="HIR5165525.1"
                     /translation="MTDTQRPDGSSLGPPPRPGAVHRHFHDELSDLKSRLLAMSADAQ
                     SALATAVDALLQRDAAIASRVIAGDRHIDAVETEIEDLVVRLLATQQPMARDLRLLMA
                     ALKIS"
     gene            complement(317..1138)
                     /gene="pstB"
                     /locus_tag="AC16JC_12325"
     CDS             complement(317..1138)
                     /gene="pstB"
                     /locus_tag="AC16JC_12325"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR5165526.1"
                     /translation="MSAAISPRPAGHLVTIPQQAVSPFPVVDVREFNFWYGERQALVG
                     LTFTIRRRAVTALIGPSGCGKSTFLRSLNRMNDLIPNTGHTGDILLEGVSVFSPGSDV
                     VDLRRRVGMVFQRPNPFPKSVFDNVAYGPRINGLAAERDMAERVERALRQAALWDEVK
                     DRLDEPGTSLSGGQQQRLCIARALANEPEMLLMDEPCSALDPIATERIEELIVELKRD
                     YTIVIVTHNMQQASRVSEFTGFFYVGRLVEFGDTAQIFTRPQDERTEGYITGRFG"
     gene            complement(1135..2001)
                     /gene="pstB"
                     /locus_tag="AC16JC_12330"
     CDS             complement(1135..2001)
                     /gene="pstB"
                     /locus_tag="AC16JC_12330"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013248948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR5165527.1"
                     /translation="MSRPPSLLESPRAGAGVSAHLPRIERSPDQGADPDDSRVRIETR
                     RLTALFGRKAAVREVSLFFPTNTVTAIIGPSGCGKSTFLRCLNRMHELSEGGWVTGSV
                     LLDGEDIYGGGLDPVAVRRRVGMVFQRPTPFPTMSIYENVAAGLRVGERRRRAELDEV
                     VERALRQAALWDEVKDRLHGSAVALSGGQQQRLCIARAVAPRPEVLLLDEPTASLDPA
                     GTQRIEELCHELKHRYTIVIVTHNMQQAARVSDYTAFFYMGELIEIGRTSRLFTNPAK
                     EQTEAYLTGRFG"
     gene            complement(2006..2851)
                     /gene="pstA"
                     /locus_tag="AC16JC_12335"
     CDS             complement(2006..2851)
                     /gene="pstA"
                     /locus_tag="AC16JC_12335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR5165528.1"
                     /translation="MSARALRRRAQSIAMTAVVAGLALLAVLPLLLIFADLVHKGANA
                     IDWNFFVKSPVPAGESGGGVANAIVGTGIIVALACLAGIPIGVGTGLFLAKYGGGRLG
                     WLVRFVADVMNGTPSIVVGISAWTWLVRPAGHFSALAGGAALAILMIPMLARTTEEMV
                     RLVPNSLREAALALGYPRWRTSLAVVTRTALAGIVTGALVAVARIAGETAPLLFTALG
                     NLNFSTGIADPMQTLSLQIYVYATGPFEEWHRLAWAAALVLMGMVLVLSLAARWATRS
                     RHSAG"
     gene            complement(2848..3723)
                     /gene="pstC"
                     /locus_tag="AC16JC_12340"
     CDS             complement(2848..3723)
                     /gene="pstC"
                     /locus_tag="AC16JC_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR5165529.1"
                     /translation="MLLPLLLLAVAGQLAVSGAPALKKFGFRFLFTTTWDPVAEVYGA
                     APMIFGTIVSSLLALLIAVPLALGVAIFLTEFAPPWLRRPVGFLVELLAAVPSVVYGL
                     WGVFVLIPLLRNVAVPPLRAALGWTPFFSGVFYGNSMLAGGVILAIMIVPYIAAVSRE
                     VLLAVPATQREAALGLGATRWEAVWSAVLPYGRAGIIGAIMLGFGRALGETMAVTMLI
                     GNRHDLGASLLSPAYTMAAAIANEFSEATTQMYLSALFAVGLVLFVITVTINALARLL
                     VWRVARGTAVGSKAL"
     gene            complement(3921..5033)
                     /gene="arsB"
                     /locus_tag="AC16JC_12345"
     CDS             complement(3921..5033)
                     /gene="arsB"
                     /locus_tag="AC16JC_12345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007194572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015700 - arsenite transport [Evidence
                     IEA];
                     GO_process: GO:0046685 - response to arsenic-containing
                     substance [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HIR5165530.1"
                     /translation="MSAQCEVTADRAGGAPLGPFERYLTLWVFLAIAGGIALGRVAPG
                     VFAVLGAAAVAHVNLVVAALIWLMIIPMLLKVDFAALRQVRAHARGMGVTLFVNWAVK
                     PFSMALLAWIFVRGVFAPLLPAAQLDSYVAGLILLAAAPCTAMVFVWSNLSDGDPNFT
                     LTQVALNDTIMIVAFAPIVSLLLGLSAITVPWETLLLSVALYIVIPVIVAQLWRRALH
                     ARGGDAALARVLERLHPLSLVALLTTLVLLFGFQGPQILQQPLVIVLLAVPILLQVYL
                     NSGLAYLLNRWLGESHRVASPSALIGASNFFELAVATAISLFGLESGAALATVVGVLV
                     EVPVMLSVVRIVTRTRGWYERRQPVTAGPQDRGQSP"
     gene            complement(5178..6239)
                     /gene="pstS"
                     /locus_tag="AC16JC_12350"
     CDS             complement(5178..6239)
                     /gene="pstS"
                     /locus_tag="AC16JC_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011938430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HIR5165531.1"
                     /translation="MRRSALLGVAAALGLSAAPAAAQVKLTGAGATFPNVIYQNWMLT
                     YNKANPSVQLNYQSIGSGGGIRQFSDKTVDFGGSDAPMSDSAIAAIQGNVLHIPTVLG
                     AVVVTYNLPGVTRQVRFSPALIADIFLGKLTKWNDARIAALNPGLGLPGMDILVVHRS
                     DGSGTSYIFTDYLAKVSPEWQSKVGKGTSVNWPVGLGGRGNEGVAATVNQTPGAVGYV
                     ELAYANSNHLPFGQLQNKAGKFINPTLEATTAALAGAMSSMSPTTDFRVSITDPDGAD
                     AYPITSMTYLLLRKQYEDPAKAAALINYVWWAETAGQAKAAALGYAPLPAALRPWIAD
                     RLASITAGGRKVWTATAAR"
     gene            complement(6258..>6921)
                     /locus_tag="AC16JC_12355"
     CDS             complement(6258..>6921)
                     /locus_tag="AC16JC_12355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165532.1"
                     /translation="EALWDYRMQGQMAMERGGYLTSSDIGFGIDGKWGPDNVNAQLTF
                     VNGEGYSGGPGDQRKDILGRVSVRLLMTDDSSRVGGLRVTGYGGYGKPTSGGQRNRFL
                     GMVSYKSKLITLAGQFATTKDSVTATPTPSTTGRVISAFGVFHIPSSKAAVIARVDIT
                     DPNTGAGGNKQTRFIGGISYQLSPNLRLLADIDHVNFEGTPTPAQEAVRSQALFQTQI
                     TF"
BASE COUNT         1189 a         2312 c         2401 g         1019 t
ORIGIN      
        1 gagatcttga gcgcggccat gaggaggcgg aggtcgcgcg ccatgggctg ctgggtggcc
       61 agcaaccgca cgaccagatc ttcgatttcg gtctcgacgg cgtcgatgtg gcgatcgccg
      121 gcgatcactc tgctggcgat cgcggcatcc cgctgcagca gggcatccac ggccgtcgcc
      181 agcgccgact gggcgtccgc cgacatggcg agcagccgcg acttgaggtc ggagagctcg
      241 tcgtggaaat gacggtgcac ggctccgggg cggggggggg gcccgaggct cgacccgtcc
      301 ggacgttggg tatcggtcat ccgaacctcc cggtgatgta gccctcggtc cgctcgtcct
      361 ggggccgcgt gaagatctgc gccgtgtcgc cgaactccac gaggcggccg acatagaaga
      421 accccgtgaa ctcggagacg cgggacgcct gctgcatgtt gtgggtcacg ataacgatgg
      481 tatagtcgcg cttcagctcg acgatcagct cctcgatccg ctcggtggcg atgggatcga
      541 gcgccgagca cggctcgtcc atgagcagca tctccggctc gttggcgagc gcccgggcga
      601 tgcacagccg ctgttgctga ccgcccgaga ggctggtgcc cggctcatcc agccgatcct
      661 tcacctcgtc ccacagcgcc gcctggcgca gggcccgctc cacccgctcc gccatgtccc
      721 gctcggcggc caggccgttg atacgcggac catacgccac gttgtcgaag acggacttgg
      781 ggaacgggtt gggacgctgg aacaccattc ccacgcgccg acgcaggtcc accacgtcgc
      841 tgccgggaga aaacaccgac acgccttcga gcaggatgtc gccggtatgt ccggtgttcg
      901 gaatgagatc gttcatgcga ttgagcgacc gcaagaacgt gcttttgccg caccccgatg
      961 gcccgatcaa cgccgtgacc gcgcgccggc gaatggtgaa cgtcaggccc accagtgcct
     1021 ggcgctcccc ataccagaag ttgaattccc gcacgtccac caccggaaag gggctgaccg
     1081 cctgctgcgg aatcgtcacg aggtgtcccg cagggcgggg cgagatggcc gcgctcatcc
     1141 gaaccgtccg gtcagatagg cttcggtctg ctccttggcc gggttagtga agagccggct
     1201 ggtccgcccg atctcgatca actcccccat atagaagaaa gcggtgtagt ccgacacgcg
     1261 cgcggcctgc tgcatgttgt gggtcacgat gacgatcgtg tagcggtgtt tgagttcgtg
     1321 acagagctcc tcgatgcgct gtgtgccggc cggatcgagc gatgccgtgg gctcgtcgag
     1381 caacaacacc tcgggccggg gggccacggc gcgggcgatg cacaggcgct gctgctggcc
     1441 gccggacagc gccacggcgc tgccgtgcaa ccggtctttc acctcgtccc acagcgcggc
     1501 ctgccgcaac gcccgctcga cgacctcgtc cagctccgcg cggcggcggc gctctccgac
     1561 gcgcagcccg gcagccacgt tttcgtagat cgacatggtc gggaacggcg tagggcgctg
     1621 gaacaccatc cccacacgac gccggaccgc caccggatcg aggccgccac cgtagatgtc
     1681 ttcgccgtcg agcaggaccg agcccgtcac ccagccgccc tcggacagct cgtgcatccg
     1741 gttgaggcag cgcaggaagg tgctcttgcc gcatcccgac gggccgatga tggccgtcac
     1801 ggtgttggtg gggaagaaga gggaaacttc ccggaccgcc gccttcctgc cgaacagcgc
     1861 cgtgagccgg cgcgtttcga tccggacgcg ggagtcatcc ggatcggcac cctggtcggg
     1921 agatcgctcg atcctcggca ggtgggcgga tacgcctgcc ccggcgcgag gcgattcgag
     1981 gagggaaggc ggccggctca tcgcgtcatc ccgcgctatg gcgtgagcgc gtagcccacc
     2041 gagccgcgag cgagagcacc aggaccatgc ccatgagtac cagggccgcg gcccaggcca
     2101 gccggtgcca ttcctcgaac ggtcccgtgg cgtacacgta gatctgcagg gagagggtct
     2161 gcatcgggtc cgcgatcccg gtggagaagt tcaggttgcc cagcgcggtg aacagcaacg
     2221 gcgccgtttc gccggcgatg cgcgccacgg ccacgagcgc gccggtcacg atgcccgcca
     2281 gtgccgttcg agtcacgacg gcgagcgacg tgcgccagcg cgggtagccc agcgccagcg
     2341 ccgcctcccg cagcgaattg ggcacgaggc gcaccatttc ttcggtcgtc cgggcgagca
     2401 tggggatcat gaggatggcc agggccgctc cgccggccag ggcggagaag tggcccgccg
     2461 gacgcaccag ccaggtccac gcggagatgc cgaccacgat cgaaggtgtg ccgttcatga
     2521 cgtccgccac gaagcgcacc agccagccca gccggccgcc gccgtacttc gccaggaaca
     2581 ggccggtgcc gacgccgatg gggatcccgg ccaggcaggc gagcgcgacg atgatgccgg
     2641 tgcccacgat cgcgttcgcg accccgccgc ccgactctcc ggccgggacc gggctcttga
     2701 cgaagaagtt ccaatcgatg gcgttcgcgc ctttgtggac gaggtcggcg aagatgagca
     2761 gcagcggcag gacggcgagc agcgccagcc ccgcgacgac agcggtcatc gcgatgctct
     2821 gggcgcggcg ccgcagggcg cgcgcgctca cagcgccttg ctccccacgg ccgtgccgcg
     2881 ggccacgcgc cagacgagga ggcgcgccag cgcattgatg gtgacggtga tgacgaacag
     2941 cacgagtccg acggcgaaca aggcggacag gtacatctgc gtcgtcgcct cgctgaactc
     3001 attggcgatg gcggcggcca tcgtgtaggc gggcgacagc agcgacgcgc cgagatcgtg
     3061 ccggttgccg atgagcatgg tgacggccat ggtctcgccc agcgcccggc cgaacccgag
     3121 catgatcgcc ccgatgatac cggcgcgccc gtacggcagc accgcgctcc agaccgcttc
     3181 ccagcgggtc gccccgaggc cgagcgcggc ctcccgctgg gtggccggga cggccagcag
     3241 cacctcccgc gagaccgcgg caatgtaggg cacgatcatg atcgcgagaa tgacgccgcc
     3301 cgcgagcatg gagttgccgt agaacacacc gctgaagaac ggcgtccatc cgagcgccgc
     3361 ccgcagcggg ggcacggcga cgttgcggag cagcggaatg agcacgaaca cgccccacag
     3421 cccgtacacg acgctgggaa ccgccgcgag cagctcgacc aggaagccga cgggccggcg
     3481 cagccagggt ggcgcgaact ccgtgaggaa gatggccacg cccagcgcca gggggaccgc
     3541 gatcagcagt gccagcaggg acgaaacgat ggtgccgaag atcatcggcg ccgctccgta
     3601 cacttcggcg accggatccc aggtcgtggt gaacaggaac cggaagccga atttcttgag
     3661 ggccggcgcc ccgctgaccg ccagctgtcc ggccacggcg agcagcagaa ggggcaggag
     3721 caacgcgaga gcggtcaata ccgcccggta agcgggatct ccgaggttgc gtggatgacg
     3781 cgccaccagg atgggcgtgc cggcggggac cgcgccagcc gtgttctctg tcttcgttgg
     3841 agttccggca gttccgtacg tcactcggcc aaggtaggcc gcggcgttct gcagccggtc
     3901 acaggcatgt aacgatcctg tcacggggat tgcccccggt cctgggggcc cgcggtcacc
     3961 ggctggcggc gctcatacca ccctcgtgtg cgggtgacga ttcgcaccac cgagagcatc
     4021 accggcactt cgaccagcac acccacgacc gtcgcgagcg ccgcgcccga ctccaacccg
     4081 aacaggctga tggcggtcgc cacggcgagc tcgaagaagt tgctggcgcc gatcaatgcc
     4141 gaaggcgagg ccacgcgatg ggattctccc agccaccggt tcaacagata ggcgagcccg
     4201 ctgttcaggt acacctgcag gagaatgggt accgcgagga gcacgatcac gagcggttgc
     4261 tgcaggattt gcgggccttg gaagccgaag aggaggacca gcgtcgtcag cagggcgacg
     4321 agggacagtg gatgcagccg ctccagcaca cgcgccaggg cggcatcccc gccgcgggcg
     4381 tgcagggccc ggcgccagag ctgcgccacg atcacgggga tcacgatgta caacgccacg
     4441 gacaggagca acgtctccca cggcaccgtg atcgccgaca ggcccagcag caggctcacg
     4501 atcggagcga aggcgacgat catgatcgtg tcgttgagcg ccacctgggt cagcgtgaag
     4561 ttggggtcgc cgtcgctcag gttgctccac acgaacacca tggcggtgca gggcgcggcg
     4621 gcgagcagga tgagcccggc cacgtaggag tcgagctggg cggcgggcag gagtggggcg
     4681 aacacgccgc gcacgaagat ccaggcgagc aacgccatcg agaacggctt gaccgcccaa
     4741 ttgacgaaca gcgtcacgcc catccctctg gcgtgggccc ggacctgacg cagcgcggcg
     4801 aagtccacct tcagcagcat ggggatgatc atgagccaga tgagcgcggc cacgaccagg
     4861 ttgacatggg cgactgcggc ggcgcccagc accgcgaaga cgccgggggc aactcgcccg
     4921 agggcgatgc caccggcgat cgcgagaaac acccacaggg tcaggtagcg ctcgaacggt
     4981 cctaagggag cgccgcccgc tcgatcggcc gtcacctcac actgggcact caaggcgact
     5041 cctcggtatc ccggccgcga gtcgcagccg ccggaccatg tctaataacc acggccccgt
     5101 gaagtccaca tgaacgcgaa acagagcccc cgcaagggtg cgccctgcgg ggggtgaccg
     5161 gcagtcgtca cggagggtta acgagctgcc gtagccgtcc agaccttgcg accaccggcg
     5221 gtgatcgatg ccagccggtc ggcgatccag gggcgcaagg ccgccggcag cggggcgtac
     5281 cccagcgcag ccgccttcgc ctgtcccgcg gtttccgccc accacacgta gttgatgagc
     5341 gctgcggcct tcgccggatc ctcgtactgc ttgcgcagca gcaagtaggt catgctggtg
     5401 atcggatacg cgtcggcccc gtcggggtcc gtgatcgaga cccggaagtc ggtggtcgga
     5461 ctcatcgagg acatcgcgcc ggccaacgcg gcggtggtcg cctcgagcgt cgggttgatg
     5521 aacttcccgg ccttattctg caactggccg aaggggaggt ggttggaatt cgcgtacgcg
     5581 agctcgacat acccgacggc gcccggggtc tgattcaccg tcgccgccac gccctcattg
     5641 ccgcgcccgc ccaaaccgac cggccagttc accgaggtgc ccttgccgac tttggactgc
     5701 cactccgggc tgaccttcgc gaggtagtcg gtgaagatgt aggaggtgcc cgagccgtcc
     5761 gaacggtgca cgacgaggat atccatgccg ggcagtccca ggcccgggtt cagggccgcg
     5821 atgcgcgcat cgttccactt ggtgagcttg ccgagaaaga tgtcggcgat cagcgccggc
     5881 gagaacctga cctgcctggt gacgcccggg aggttgtagg tgacgacgac cgcgcccagg
     5941 acggtgggga tgtggagcac gttgccctgg atggcggcga tcgccgagtc ggacatgggg
     6001 gcgtcgctgc cgccgaaatc gacggtcttg tcggagaact ggcggatgcc gccacccgag
     6061 ccgatggact gatagttcag ctgcacgctc gggttcgcct tgttgtaggt cagcatccaa
     6121 ttctgataaa tgacgttcgg gaacgtcgcg ccggcgcccg tgagcttcac ctgggcggcg
     6181 gccggggcag cgctcagtcc gagcgccgcc gccacgccga gtagtgcact tcgtctcatc
     6241 gttgcctctt gttcaggtca gaaggtgatc tgcgtctgga acagcgcctg cgagcgcacc
     6301 gcttcctgcg cgggcgtcgg cgttccctcg aagttcacgt ggtcgatatc ggccagcagc
     6361 cggaggttcg ggctcaactg gtaggagata ccgccgatga accgcgtctg tttgttgccc
     6421 ccggcgccgg tgttcgggtc ggtgatgtcc acccgggcga tgaccgcggc cttggagctc
     6481 gggatgtgga acaccccgaa ggcggagatc acccggccgg tggtggaagg cgtcggggtc
     6541 gcggtgacgc tgtccttggt ggtggcgaac tgaccggcga gcgtgatcag cttgctcttg
     6601 taggagacca tgccgaggaa ccggttgcgc tgcccgccgg aggtcggctt cccgtagccg
     6661 ccgtagccgg tcacgcgcag tccgccgacg cggctcgaat cgtcggtcat caggagacgg
     6721 acggagacgc gtcccaggat gtccttgcgc tggtctccgg ggccgccgct gtacccttcg
     6781 ccgttcacga acgtcagctg cgcgttgacg ttgtccggcc cccatttgcc gtcgatgccg
     6841 aatccgatat cggaggacgt gaggtatccg ccgcgctcca tcgccatctg cccctgcatc
     6901 cggtagtccc acagcgcctc t
//
LOCUS       DBNGZW010000520         8311 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1683147, whole genome shotgun sequence.
ACCESSION   DBNGZW010000520 DBNGZW010000000
VERSION     DBNGZW010000520.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8311)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8311)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8311
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1683147"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1229)
                     /locus_tag="AC16JC_12360"
     CDS             complement(<1..1229)
                     /locus_tag="AC16JC_12360"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR5165533.1"
                     /translation="MGGRTTIGGTACITKAVMKKRAQYSAPLARRQRALAIILGATLL
                     VAAPGCRPKPVDQQALLTARDLGLTDLQRGRLPEAEVEFKQVVALAPRDPLGYADLGL
                     TYLRGGRYAEAETQLRRARRLDPANPEIGLIAARLYALTGRPDKARATLGELRRDAKV
                     LYALAELAAQHADSGAMRQYEEQLRQVLGLVPANVAVRLNLVDVLVRRGETDSAVQHL
                     EAMRRLRPEPPPEAHPYLDATIQHLRAGRLAAARAALDRFLHLMELTAPYQAALNEVQ
                     WVEGPLVGRPVIAFNPQTLIATRTLIPVGRKPDVRFTDVTDEVGLSDAGTAPSALALG
                     DYDGDGEDDLFLSTTSRDRRSAARLYAAHGGFVVDVTARAGLALPAGAVYATFADFDN
                     DGWLDLFAIGGDGRGYLL"
     gene            1265..4618
                     /locus_tag="AC16JC_12365"
     CDS             1265..4618
                     /locus_tag="AC16JC_12365"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HIR5165534.1"
                     /translation="MPARYRHPSGLILLAGVLACASCERTAGRTLFEPVPPEASGVAF
                     VNALPEDTAFNILNYLYYYNGGGVAAGDVDGDGLPDLYFTSNLGPNRLYRNTGHFRFE
                     DITERAGVADSGGWKSGVTMADVNGDGWLDIYVSGVSYLGMHGRNVLYINNGDGTFTD
                     RTEEYGVGFAGYSTQAAFFDYDGDGDLDMYLLNNSTHTERAASGNTSRTVRNAKAGDR
                     LYRNDGGHFTDVSERAGIYGGVEGYGLGVVASDVNLDGCLDLYVANDFQENDFLYINN
                     CDGTFTESIARATGHTSRFSMGVDAADFNNDGRPDVIVLDMLPERQDILNTSAGAEDY
                     SVFELRLKAGYHPQYARNTLQLNRGLVGGTVRFSEIGYLAGVFATDWSWAPLFADLDD
                     DGYKDLFVTSGIYRRPNDLDYINFVSTEAVQASLAAGITTENLTPLLAKMPHIPIANH
                     AYRNNGDLTFTDMANRWGLGQPGFSNGAAYVDLDNDGALDLVVNNLNAPAAIYRSRAR
                     AITGNHYLQVLLRGTGANTAAIGAKVMIVHQGMRQLLEQMPTRGFQSSVDLRLHFGLG
                     RSGSIDSLIVIWPDRRVQVLTRVAADQVITLSQRDAAGKYADQQGAGSPPLFADVTAR
                     IGIGVRHEENTFYDYGREPLMPHLLSTEGPALATGDVNGDGRDDIFVGGAKWQAGRLL
                     VQRPDGGFRESSQPAFQADSLAEDVSAVFFDADGDGDPDLFVVSGGNEFWDDAEALRD
                     RLYLNDGRGNFHRAEHALPDFFDNGSCVVPGDFNGDGRLDLFVGGRVVSRKYGLTPRS
                     HLLVNDGGAVAHFSDVTLAQAPGLAEAGMVSSAAWIDYDNDGRLDLIVAGEWMPVRVF
                     RQENGRFVDRTAAAGLAGTNGWWNSVTAADLNGDGRPDLVLGNLGLNSYIRASRAEPA
                     RLYVRDFFGNGTLEQILTFYKRGVSYPLAGRDELVRLMPQLRSKYPSYAAFGASRIGD
                     IFPASELRRATVLEADDFASSVALNHGDGTFDLRPLPPEAQFAPVYASLAEDFDGDGR
                     TDLLLAGNFYGVTPVQGRYDASYGVLLRGDGTGRFAAVDPEASGLAIEGQVRHMALQR
                     RAGGDRLIVVARNGDRLQGLLERATSAKR"
     gene            4734..6818
                     /locus_tag="AC16JC_12370"
     CDS             4734..6818
                     /locus_tag="AC16JC_12370"
                     /inference="COORDINATES: protein motif:HMM:NF024827.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HIR5165535.1"
                     /translation="MAQVDLRPRLTEWQHRLLLCVAGLLTFETLTGLSIYLLPFSVPN
                     QVMVLLHTAIGLVFVVPYAWYQIRHWRLYRGRQMTHVVLTGYFSMAAAIALAVSGLVL
                     SYQALLASRIGYGWDLAHLIATFALVASVLPHVLTLVYHALRGHNEEALELRRGQKQL
                     GLKTVYVTVALLAVVALGTYAYRPVRLVNRLPADYSFIYGPDRPFAPSLARTSTGQAF
                     DARSTGGSKSCGSSGCHEQIYREWAVSAHRYSAMDLGFRAIQTTMGKQNGPESTRYCA
                     GCHDPISLFAGTKNLYRDDLTNAIGRDEGVSCIVCHSIKETDVKGNASYLITQPQRYL
                     FELSDSGGTRLVRDFLIRAYPRQHVRSLEHRLFKSPQFCAACHKQFIDEEINQVGWVQ
                     LQNQYDNWRKSRWNDTINPRKTIECRECHMPLQDSHDPARGDNLDYNRSSGDGKHRSH
                     QFLAANQFMPLALKLDGAQEHANLTERWLQGRIAIPEIADKWRTGPAVPIELIAPAQV
                     RPGEQIHLAVAITNNKVGHDFPTGPLDIIQSWVEVVVRDQDGNVVFATGQRDKQHFIE
                     PGSFMFKSEPVDQYGKLIDRHNLWEMVGVRYRRALFPGQSDRAEFAFHYPTTASRGPR
                     ATTGVPGQQTFAFRAPGRVVRLDVTAKLMYRKIDQFLLNFLFGESAGITAPVTVLSQD
                     IKSIEVVPNGGR"
     gene            6824..>8311
                     /locus_tag="AC16JC_12375"
     CDS             6824..>8311
                     /locus_tag="AC16JC_12375"
                     /inference="COORDINATES: protein motif:HMM:NF024907.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP repeat domain-containing protein"
                     /protein_id="HIR5165536.1"
                     /translation="MSAALPRWKRRLVLTGALALAFVAVAGTIVFLAVKPGPAYRPGA
                     KIEGLTSELSRSLPADYPRITFTDVTREAGIQFRHFSGQRSSQIPEDMGSGAAWGDYN
                     NDGWPDLFLVNVVGPITLTTAQVRQSPAHCALYRNNRDGTFTDVSVEAGVDLRAWGMG
                     AEWADYDNDGRLDLFVSTYGENVLYHNNGDGTFSDRTRAAGLGGKRGFWSGVAWGDYD
                     RDGFLDLYVTGYVKYSRPSEPTAAEQYDVENPASINPNAFPPERNLLYHNNRNGTFTE
                     VAARAGVLGNRGKSLEAAWADFDEDGWPDLYVANDVTDNQLFRNLGNGTFEDISHLAH
                     VADYRSAMGLAVGDWNGDGHLDIYITHWIAQENALYTNLLKPRSGGGRGAALEFRDEA
                     DRYGLGQVTLDFVGWGTFFFDYDNDGRPDLFVANGHTFQRRDAPHLLAPETAQLFWNG
                     GPDRGFYDVSAVSGEYFHNPYVGRGAAFADYDHDGDLDVVVVNHGG"
BASE COUNT         1403 a         2775 c         2790 g         1343 t
ORIGIN      
        1 agcaggtaac cacgaccgtc gccgccgatg gcgaacaggt cgagccagcc atcgttgtcg
       61 aaatcggcga acgtggcgta gacggcgccg gcggggagcg cgaggcccgc ccgcgccgtg
      121 acgtccacca caaaaccgcc gtgcgcggcg tagagacggg ccgcggaacg ccgatcgcgg
      181 gaagtggtcg agaggaagag atcgtcctcg ccgtcgccgt cgtagtcgcc cagggcgagc
      241 gcggagggcg ccgttcccgc gtcggagagg ccgacctcgt ccgtcacgtc ggtgaagcgc
      301 acatcgggct ttcggcctac gggaatcagc gtacgcgtgg cgatcagcgt ctgcgggttg
      361 aaggcgatga ccggtcgtcc gacgaggggg ccttcgaccc actgcacttc gttcagggca
      421 gcctggtacg gggcggtgag ctccatgagg tgcaggaagc gatcgagcgc cgccctcgcc
      481 gccgcgagcc ggccggcgcg caggtgctgg atcgtggcgt cgagatacgg gtgggcctcg
      541 ggcggcggct cggggcgaag ccgacgcatc gcttcgaggt gctgcaccgc gctgtcggtc
      601 tccccacggc gcacgagcac atccaccagg ttgagccgga cggcgacatt cgccggcacc
      661 aagccgagga cctgtcgcag ctgctcctcg tactggcgca tggcaccgct gtccgcgtgc
      721 tgcgctgcga gctcggcgag cgcgtacaac accttggcgt cgcgccgcag ctcgccgagg
      781 gtcgcgcgcg ctttgtcggg tcgtccggtg agcgcataga gcctggcggc gatgagtccg
      841 atctcggggt tggccggatc cagccggcgc gcgcggcgca actgggtttc ggcttccgca
      901 tagcgaccgc cgcgcaagta ggtgaggccc aggtccgcgt atccgagcgg atctctcggc
      961 gccagcgcca cgacctgttt gaattccact tcggcttccg gcaaccggcc ccgctggagg
     1021 tcggtgagcc cgaggtcgcg cgctgtgagc agtgcctgct ggtcgaccgg cttgggccgg
     1081 cagccggggg cggccacgag caacgtcgcg ccgaggatga tggcgagcgc cctttgccgc
     1141 cgcgcaagcg gggcactata ctgcgcccgc ttcttcatca ccgctttcgt gatgcacgcc
     1201 gttccgccga tcgtggtgcg tccgcccacg ccggcgcccc ccgcagaaag ggtggtaagt
     1261 ctggatgccc gcacgatatc gtcatccctc ggggttgatc ctgctcgctg gggtgttggc
     1321 atgcgcgagc tgtgaacgca ccgccggtcg gaccctgttc gagccggtcc caccagaagc
     1381 gagcggtgtc gcattcgtca acgcgctgcc cgaagatacg gcgttcaaca tcctgaacta
     1441 cctgtactac tacaacggcg gcggcgtcgc ggccggcgac gtcgacggcg acggcctgcc
     1501 ggacctgtac ttcacgtcga acctggggcc caaccgcctc tatcgcaaca cggggcactt
     1561 ccgcttcgag gatatcaccg agcgcgccgg tgtggcggac tccgggggct ggaagtccgg
     1621 ggtcacgatg gccgacgtca acggcgacgg gtggctcgac atctacgtct ccggcgtcag
     1681 ctacctcggc atgcacggcc gcaacgtgct ctacatcaac aacggagatg gaacgttcac
     1741 cgaccgcacc gaggaatacg gggtcgggtt tgccggctat tccacgcagg ccgccttctt
     1801 cgactacgat ggcgacggcg acctggacat gtacctcctg aacaactcca cccacaccga
     1861 gcgcgccgcc agcgggaaca cgagccgcac ggtgcgtaac gcgaaggcgg gagaccggct
     1921 gtaccgcaac gacggcggcc acttcacgga cgtcagcgag cgagccggca tctacggcgg
     1981 ggtcgaggga tacgggctgg gcgtcgtggc gagcgacgtg aatctcgacg gctgcctcga
     2041 cctgtacgtc gccaacgact tccaggagaa cgactttctc tacatcaaca actgtgacgg
     2101 cacattcacg gagtcgatcg cccgggcgac ggggcacacc agccgcttct cgatgggcgt
     2161 cgacgcagcc gatttcaaca acgacggccg tccggacgtc atcgtcctgg acatgctgcc
     2221 cgaacgacag gacatcctca acacgtcggc cggcgcggaa gactacagcg tcttcgagct
     2281 gcggctcaag gccggctacc atccacagta cgcccgcaac acgctgcagc tgaaccgcgg
     2341 tctggtgggc ggaacggtac gcttcagcga gatcggctac ctggccggcg tctttgccac
     2401 cgactggagc tgggcccccc tgttcgccga cctggacgac gacggctaca aggacctgtt
     2461 cgtcaccagc gggatctacc gccgccccaa cgacctcgac tacatcaact tcgtgagcac
     2521 cgaggccgtt caggcctcgc tcgcggcggg gatcaccact gagaacctga cgccgctcct
     2581 cgccaagatg ccgcacatcc cgatcgcgaa ccacgcgtac cgcaacaacg gggatctgac
     2641 gttcacggac atggcgaatc ggtgggggct ggggcagccg ggcttctcca atggggcggc
     2701 ctacgtcgac ctggacaacg acggcgcgct cgatctcgtc gtgaacaacc tcaacgcacc
     2761 ggcggcgatc taccggagcc gagccagggc gatcaccggg aatcactatc tccaggtgct
     2821 gctgcggggc acgggcgcca acacggccgc catcggcgcc aaggtgatga tcgtgcatca
     2881 gggcatgcgg cagctgctgg agcagatgcc cacgcgcggc tttcagtcgt cggtggatct
     2941 ccgcctgcat ttcgggctgg ggcgctcagg atcgatcgat tcgctcatcg tgatctggcc
     3001 cgatcgtcgt gtccaggtcc tgacccgcgt cgcggccgac caggtgatca cgctctccca
     3061 gcgggatgcg gcagggaagt acgccgacca gcagggcgcc ggatcgccgc cgctgttcgc
     3121 ggacgtgacg gcccgcatcg gcatcggcgt caggcacgaa gagaacacgt tctacgacta
     3181 cggccgcgag ccgctcatgc cgcatctgct gtccaccgag gggccggccc tggccacggg
     3241 cgacgtgaac ggcgacgggc gggacgacat cttcgtcggc ggtgccaagt ggcaggccgg
     3301 tcgcctgctg gtccagcgtc ctgacggcgg gtttcgcgag agcagccaac ctgcgttcca
     3361 ggcggacagc ttggccgagg acgtaagcgc ggtgttcttc gacgccgacg gggacgggga
     3421 tcccgacctg ttcgtcgtga gcggcggcaa cgaattctgg gacgacgccg aggcgctccg
     3481 cgaccggctc tatctcaacg atgggcgggg caacttccac cgggcggagc acgccctccc
     3541 ggatttcttc gacaacggct cctgcgtcgt cccgggcgat ttcaacggcg acggacggct
     3601 cgatctcttc gtcggcggcc gggtcgtctc gcgcaagtac ggcctcacgc cccggagcca
     3661 tctgctcgtg aacgacggcg gggcggtcgc gcacttcagc gacgtcacgc tcgcgcaggc
     3721 gccggggctc gccgaggcgg gcatggtctc gtcggccgca tggatcgatt acgacaacga
     3781 tgggcggctg gacctgatcg tcgccggcga atggatgccg gtccgggtgt ttcggcaaga
     3841 aaacggccgg ttcgtggacc ggaccgcggc ggccggtctc gcgggcacga acggctggtg
     3901 gaacagcgtc acggcggccg atctgaacgg cgacggccgc ccggacctgg tgctgggcaa
     3961 cctcgggctc aactcctaca tccgggcatc gcgcgcggag cccgcccggc tgtacgtccg
     4021 tgatttcttc gggaacggca cgctcgagca gatcctgacc ttctataaac gcggcgtgag
     4081 ctatccgctc gcggggcgag acgagctggt gcgtctgatg ccgcagttgc ggagcaagta
     4141 cccgtcctat gccgcgttcg gggccagccg gatcggggat attttcccgg cttcggagct
     4201 gcggcgggcc acggtgctcg aagcggacga tttcgcgagt tcggtggcgc tcaaccacgg
     4261 ggacggcacc ttcgacctcc gtccgctccc tcccgaagcg cagttcgctc ccgtctatgc
     4321 gtcgttggcg gaagacttcg acggcgatgg gcgcaccgat ctcctcctcg cagggaactt
     4381 ctacggcgtg acgccggtgc aggggcggta cgacgcgagc tatggcgtgc tgctgcgcgg
     4441 cgacggcacg gggcgcttcg cggccgtcga tccggaggcg agcgggctgg ccatcgaagg
     4501 acaggtgcgg cacatggcgc tgcagcggcg cgccggcggc gaccgcctga tcgtcgtggc
     4561 gcgcaacggc gaccggctcc agggcctgct cgagagagcg acgagcgcga agcggtagcc
     4621 atgcggtgca tgaactcccc ttcacagaat cttccttgtt cgttcacgcc cggcttctgg
     4681 acggagactt cagcgcaggg ttagtatcgc atcccaacca gggggagact ctcgtggctc
     4741 aggtcgatct ccgtccgaga ctcaccgagt ggcaacaccg gttgttgctg tgcgttgccg
     4801 gcctcctgac gttcgagacc ctgacgggtc tttcgatcta cctgctcccg ttcagcgtgc
     4861 cgaaccaggt gatggtgttg ctgcacaccg caatcggcct ggtcttcgtc gtcccctacg
     4921 catggtatca gatccgacac tggcgcctgt accgcgggcg ccagatgacg cacgtcgtgc
     4981 tgaccgggta cttctccatg gcggccgcga tcgcgctggc cgtttcgggg ctggtgctct
     5041 cgtatcaggc tctcctggcg agccggatcg gctacggctg ggatctcgcc cacctgatcg
     5101 ccaccttcgc gctggtcgcg tcggtgttgc cgcacgtcct gaccctcgtt tatcacgcgc
     5161 tgcggggcca caatgaggag gcgttggagc tgcgccgggg acagaagcag ctgggattga
     5221 agaccgtgta cgtgacggtc gctctcctgg ccgttgtggc gctggggacg tacgcgtacc
     5281 gccccgtccg cctcgtgaac cggctcccgg ccgactacag cttcatctat ggccccgacc
     5341 gcccgtttgc gccgagtctg gcccgcacca gcaccggtca ggccttcgat gccagatcca
     5401 cgggcggctc caagtcgtgc ggctcgtccg gctgccacga acagatctat cgggaatggg
     5461 ccgtgagcgc gcaccgctac tcggccatgg acctcgggtt tcgcgcgatc cagaccacca
     5521 tgggaaagca gaacgggccc gaatcgacac gctactgcgc cggctgccac gatccgatat
     5581 ccctgttcgc cggcaccaag aacctgtacc gcgacgatct gacgaatgcc atcggacgcg
     5641 acgagggagt ctcttgcatc gtctgtcact ccatcaagga gaccgacgtc aagggcaacg
     5701 ccagctacct catcacacag ccgcagcggt acctgttcga gctgagcgat agcggcggca
     5761 cccggctggt gcgggatttt ctgattcggg cctaccccag gcagcacgtg cgctcactgg
     5821 agcatcgcct gttcaagagc ccgcagttct gcgccgcctg ccacaagcag ttcatcgacg
     5881 aggaaatcaa ccaggtcggc tgggtgcagc tgcaaaacca gtacgacaac tggcgcaaga
     5941 gccgctggaa cgacacgatc aacccgcgca agacgatcga atgccgtgag tgtcacatgc
     6001 cgctccagga ctcgcacgac ccggcgcggg gcgacaacct ggactacaac cggtcgtccg
     6061 gcgacgggaa gcaccgcagc catcagttcc tcgcggccaa ccagttcatg ccgctggcct
     6121 tgaagctcga cggcgcccag gagcacgcca atctgaccga acggtggctg cagggccgga
     6181 tcgccatccc cgagatcgcc gacaaatggc gcaccgggcc ggcggtgccg atcgagctga
     6241 tcgcaccggc gcaggtgcgg ccgggtgaac agattcacct cgcggtggcg atcaccaaca
     6301 acaaggtcgg ccatgacttc cccaccgggc cgttggatat catccagtcg tgggtcgaag
     6361 tggtggtacg tgatcaggac ggcaacgtgg tgttcgcaac ggggcagcgg gacaagcagc
     6421 atttcatcga gcccggctcg ttcatgttca agtcggagcc cgtggatcag tacggcaaac
     6481 tgatcgaccg ccacaacctc tgggagatgg tgggcgtgcg ctaccggcgg gccctgttcc
     6541 ccggccagtc ggaccgggcc gagttcgcgt tccactaccc cacgacggcg tcgagaggac
     6601 cccgggcgac gacgggcgtg cccggtcaac agacgttcgc attccgcgcg cccgggcggg
     6661 tggtgcgact cgatgtcacg gcgaagctga tgtatcgcaa gatcgaccaa ttcctcctga
     6721 acttcctctt cggcgagtcg gctggcatca ccgccccggt gacggtcctc tcgcaggaca
     6781 tcaagagcat cgaggtcgtg ccgaacggcg ggcggtagga cccgtgtccg ccgcgctacc
     6841 gcgctggaag cgccggctcg tgctcacggg ggcgctggcg ctcgccttcg tcgccgtcgc
     6901 cggaacgatc gtgttcctcg ccgtcaaacc ggggccggcc taccggcccg gtgcgaagat
     6961 cgaaggactc acctccgagt tgtcacgttc gttacccgcg gactatcccc ggatcacgtt
     7021 caccgacgtg acgcgcgagg ctggaatcca gtttcgccat ttttcggggc agcggtcgtc
     7081 tcagatcccg gaggacatgg gctcgggggc ggcatgggga gattacaaca acgacggctg
     7141 gccggacctg ttcctcgtga acgtggtcgg cccgatcacc ctgaccaccg cacaggtgcg
     7201 ccagtccccg gcgcattgtg cgctctaccg caataaccgg gatgggacgt tcaccgatgt
     7261 cagcgtggaa gccggcgtcg acttgcgcgc ctgggggatg ggtgcggagt gggcggacta
     7321 cgacaacgac ggccggctcg acctgttcgt ctccacgtac ggggagaacg tgctctacca
     7381 caacaacggc gatggcacct tcagcgatcg cacccgcgcg gcgggcctcg gcggcaaacg
     7441 ggggttctgg tcgggcgtgg cctggggcga ttacgaccgg gatgggtttc tcgacctgta
     7501 cgtgacggga tacgtcaagt attcccgtcc ctccgagccg accgccgccg agcaatacga
     7561 cgtggagaac ccggcgagca tcaatcccaa cgcgtttccg ccggagcgca acctgctcta
     7621 ccacaacaac cggaacggca cgttcaccga ggtcgctgcc agggccggcg tcctgggcaa
     7681 tcgcgggaag agcctcgagg cggcgtgggc cgatttcgac gaggacggct ggcccgacct
     7741 gtacgtggcg aacgacgtca ccgacaacca gctgttccgg aacttgggga acggcacgtt
     7801 cgaagacatc agtcacctcg cgcacgtcgc ggactaccgc agcgcgatgg ggctggccgt
     7861 gggtgactgg aacggcgacg ggcacctcga catctacatc acccactgga tcgcccagga
     7921 gaacgcgttg tacaccaatc tcctgaagcc acgctccggc gggggacggg gcgcggcgct
     7981 ggaattcagg gacgaggcgg accggtacgg gctggggcag gtcaccctcg acttcgtcgg
     8041 gtggggcacg ttcttcttcg actacgacaa cgacgggcga cctgacctct tcgtcgccaa
     8101 cgggcatacg tttcagcggc gggacgcgcc ccacctcctg gcgcccgaga ccgcgcagct
     8161 cttctggaac gggggtcccg accggggctt ctacgacgtg tcagccgtca gcggcgagta
     8221 cttccacaac ccgtacgtcg gccggggcgc cgcttttgcc gactacgatc acgatggaga
     8281 tctcgacgtc gtcgtggtga accacggcgg c
//
LOCUS       DBNGZW010000521         5312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_718523, whole genome shotgun sequence.
ACCESSION   DBNGZW010000521 DBNGZW010000000
VERSION     DBNGZW010000521.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5312
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_718523"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="AC16JC_12380"
     CDS             <1..1202
                     /locus_tag="AC16JC_12380"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF037628.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008409 - 5'-3' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HIR5165537.1"
                     /translation="LPGDALPPAAAVLDPRRPDCPSPDKDLCGTGVVFKLAQALVPVL
                     GLPEQLPWHFLDYVALATVADVVPLTGENRILVRHGLKLLAASRWTGLAALVEAAGLS
                     GKPLRAAHVGFILAPRLNAAGRIGDAGDGLRLLLTDDPDEAARLARELETLNARRQEM
                     DQQILAAAEARLASALGADDRAIVLAADDWHPGVIGIVASRLVERYGRPTFLVAWDET
                     GGLGRGSGRSIPGFDLHAALHRVGHCLERYGGHRMAAGLTVRRDRFEEFRIAFLEVAG
                     QLLSPDDLAPAQRVDLELPLGDLSEELARLIRHLEPCGAGNPAPVFGVRGARAVRARR
                     VGSNHLRFVLNDGSGAIPAIGFQVADRVPEEWLSRPLDVAFRLERDEWLGRSTLQARV
                     ATIAPSE"
     gene            1361..1885
                     /gene="rsmD"
                     /locus_tag="AC16JC_12385"
     CDS             1361..1885
                     /gene="rsmD"
                     /locus_tag="AC16JC_12385"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:NF015559.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HIR5165538.1"
                     /translation="MISIIAGEFKNRRLKTPAGDKVRPTASRVREAWFSIIQRNLRGA
                     RVLDLYAGSGALGLEALSRGAVKADFVEVHRNAIAALKANLKALKVEDRATIHKVDAL
                     KFAEQLHAGQYDVAFADPPYAMVNAQAARLVAMFRLVPFARVFSIEHPADTPIPGDDT
                     RRYGDTAITFIYAP"
     gene            1882..2370
                     /gene="coaD"
                     /locus_tag="AC16JC_12390"
     CDS             1882..2370
                     /gene="coaD"
                     /locus_tag="AC16JC_12390"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HIR5165539.1"
                     /translation="MTAAPRIAVYPGSFDPVTRGHEDLIRRSLAFVDRVIVAVAVNVA
                     KQPLFTLEERVALLHETVRLPEVEVQSFEGLLVEFAQRVGASVIVRGLRAVSDFEYEF
                     QMALMNRTLAPRLETVFLVPAFDLTYLSSSLVREVARFGGDVSQLVQPAVQQALRRKF
                     GR"
     gene            2367..3284
                     /locus_tag="AC16JC_12395"
     CDS             2367..3284
                     /locus_tag="AC16JC_12395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020636780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HIR5165540.1"
                     /translation="MSFRDRVYERARRANRRVVLPEGDDPRVRAAGARIEALGLGRVR
                     VLEGPEDASGPPLPELARLLMSRRPDRFPSNAAALQALTQPIVVAACMVGAGLADAMV
                     GGAGAPTGDVIRAALLAVGPAPGITTVSSAFYMVKDDAVLTFTDGAVVPDPTAEQLAD
                     IALAAARDRRRIVGDVPRVAFLSYSTKGSAEGVRVEKVRRAVARFREFAPDVACDGEL
                     QGDAALVADVARRKAPGSPVAGQANVLVFPDLDSGNIAYKLVQRLGGWDALGPILQGI
                     ARPIADLSRGASVEDIVDTAAVTVLQAEG"
     gene            3286..3621
                     /locus_tag="AC16JC_12400"
     CDS             3286..3621
                     /locus_tag="AC16JC_12400"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR5165541.1"
                     /translation="MGFSLSKDPRGVVIVGVDGQLIVGNRQELKQKVLDSLEGGTRRF
                     VIDFTKTGYIDSSGLGVLVSLSKKVREQGGELRLAGLNEDLQTLFELTKLDTLFAIAK
                     TPAEALAAF"
     gene            3634..4098
                     /locus_tag="AC16JC_12405"
     CDS             3634..4098
                     /locus_tag="AC16JC_12405"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5165542.1"
                     /translation="MNHQLQVVVHRSARVASPGADVAVSLSVPSDLGFVGDAVELAAR
                     YCDTGVLSPRRLQFNLRTALAEALANAIEYGNRRDPAKRVRVEIACGREAVRIDVADD
                     GAGFDPEGVPDPTAPENLERETGRGLFVLRHLVDQVEFNERGNRVCLVLLAG"
     gene            4074..>5312
                     /locus_tag="AC16JC_12410"
     CDS             4074..>5312
                     /locus_tag="AC16JC_12410"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR5165543.1"
                     /translation="MSRPAGGLTKLERLLPALEQLGGASLALRVWRAEGAAVRLVTGT
                     PAAWTPSLGEGAARLDTPDGPAWFVPMPAPGQSRGGPTLWLELRSGKELTPESRESLV
                     EAVACLAEAEREAGQVAAELSDRYEEIDLVYTISEILGHTIRLDEAARRILQEVATVV
                     GARRASLFVLDLDRHVLRIVASKGMVTQDVDPIELDDPVSVTARAFREMRIQSNDPAE
                     PEPAAARGQTGRSYRGRAFLAVPVMYGAPGDQARPIGVINLTDRHGPDLFSAGDRKLV
                     AAIANQVGAAIENARLVARDLGQQRVRRELELAHDLQLKLLPSPAVLGDKADLAARCR
                     PADSVGGDFYNLLRLHGDRVGIMIGDVSNHGFGAALIMALAMAASGIHAESSAAPAQV
                     LGSLEQSLAAELARTEMYLSL"
BASE COUNT          722 a         1809 c         1961 g          820 t
ORIGIN      
        1 acctccccgg cgacgcgctg ccgcccgccg ccgccgtgct cgatccgcgg cgtcccgatt
       61 gtccgtcgcc ggacaaggat ctgtgcggca ccggcgtcgt cttcaagctc gcgcaggctc
      121 tcgtgcccgt gctcggactt cccgagcagc tcccctggca tttcctcgac tacgtcgcgc
      181 tggcgacggt ggccgacgtg gtgccgctca cgggggagaa ccggatcctc gtccggcacg
      241 gcttgaagtt gctggccgcg agccgctgga ccgggctcgc ggcgctcgtg gaggcggccg
      301 gactctcggg caagccgctg cgcgccgccc acgtcggctt catcctcgcg ccacggctca
      361 atgccgccgg gcgcatcggc gacgccggcg acgggctccg gctgctgctc accgacgacc
      421 ccgacgaggc ggcccgtctg gcgcgcgagc tggagaccct gaacgcgcgg cggcaggaga
      481 tggatcagca gatcctcgcc gccgccgagg cgcggctcgc gagcgcgctc ggcgccgacg
      541 accgggcgat cgtgctggcc gccgacgact ggcaccccgg cgtgatcgga atcgtggcga
      601 gccgtctggt agagcgctac ggccgtccga cgtttctcgt cgcgtgggac gaaacgggcg
      661 gcctcgggcg cggctcggga cgcagcatcc ccgggttcga tcttcatgcg gcgctgcatc
      721 gcgtcggcca ctgcctcgag cgctacggcg gccatcggat ggcggccggc ctgacagtgc
      781 ggcgcgaccg cttcgaggag ttccgtattg cttttctgga ggtggcggga cagcttctca
      841 gccccgacga cctggcgccc gcccagcggg tggatctgga gctgcccctc ggcgacctga
      901 gcgaggagct ggcgcgcctc atccgtcacc tcgagccgtg cggagccgga aaccccgcgc
      961 cggtgttcgg ggttcggggg gcgcgagcgg ttcgcgcacg gcgcgtcgga tcgaatcatc
     1021 tgcgcttcgt gctgaacgac gggtcgggtg cgatcccggc catcggtttc caggtggccg
     1081 accgggtccc cgaggagtgg ctctcccggc cgctcgacgt ggcgttccgg ctggaacggg
     1141 acgagtggtt ggggcgatcg acgctgcagg cgcgcgtggc gaccatcgct ccaagcgagt
     1201 agacattgcg gattgtggat tgcggaatgc ggattgggtg caacggaggg accgagttcg
     1261 tgtgccacgc ggggcgtcag gtcagtcttg ctaggcggca tccgatccgc aatccgcaat
     1321 ccgcaatccg caatcggagg gggcgaacgg aggggacgag ttgatcagta tcatcgcggg
     1381 cgagttcaag aaccggcgtc tcaagacccc ggcgggcgac aaggtgcgtc ccaccgccag
     1441 ccgggtgcgc gaagcctggt tcagcatcat tcagcgcaac ctgcggggcg cgcgcgtgct
     1501 ggatctctac gccggctccg gggcgctggg actcgaagcg ttgtcgcgcg gtgccgtgaa
     1561 ggccgatttc gtcgaggtgc accgcaacgc gatcgccgcc ctgaaggcga acctgaaggc
     1621 cctcaaggtc gaggatcggg cgaccatcca caaggtggac gccctgaagt tcgccgagca
     1681 gctgcacgcg gggcagtacg acgtcgcgtt cgccgatcct ccgtacgcga tggtgaacgc
     1741 ccaggcggcg cgcctcgtcg cgatgtttcg cctcgtgccg ttcgcgcggg tcttctcgat
     1801 cgagcatccg gcggacacgc ccatccccgg tgacgatacg cgccgctacg gcgataccgc
     1861 gatcacgttc atctacgccc catgacggcg gccccccgca tcgccgtgta tccggggtcg
     1921 ttcgatccgg tgacgcgcgg gcacgaggac ctgatccgtc gctcgctcgc cttcgtggac
     1981 cgggtgatcg tcgcggtcgc ggtgaacgtc gcgaagcaac cgctgttcac cctggaagag
     2041 cgcgtcgcgc tgctccacga gacggtgcgg ctccccgagg tggaggtgca gtcgttcgag
     2101 gggctgttgg tggagttcgc ccagcgcgtc ggcgcttccg tcatcgtgcg cgggttgcgg
     2161 gcggtgagcg atttcgagta cgagttccag atggcgctca tgaaccgcac gctcgcgccg
     2221 cggctcgaga cggtgttcct cgtgccggcg ttcgatctca cctatctcag ctccagcctg
     2281 gtgcgcgaag tcgcccgctt cgggggcgac gtctcgcagc tggtccagcc ggcggttcag
     2341 caggcgctgc gcaggaaatt cggccggtga gcttccgcga ccgcgtctac gagcgggcgc
     2401 ggcgtgccaa tcggcgggtc gtgctccccg aaggggatga cccgcgcgtg cgggccgccg
     2461 gcgcgcggat cgaggcgctg ggcctcggcc gggtccgggt gctggagggc cccgaggacg
     2521 cgtccggccc gccgcttccc gagctggccc ggctgctcat gtcgcggcgg cccgaccggt
     2581 ttccctcgaa tgccgcggcc ctccaggcgc tgacgcagcc catcgtggtg gcggcgtgca
     2641 tggttggggc gggactcgca gatgcgatgg tcggcggcgc gggcgccccg acgggggacg
     2701 tcatccgcgc ggccctgctg gccgtcggtc cggcgcccgg cattacgacg gtgagcagtg
     2761 ccttctatat ggtgaaggac gacgccgtgc tcaccttcac cgacggcgcc gtcgtgccgg
     2821 acccgacagc ggagcagctg gccgacatcg cgctcgccgc cgcgcgcgac cggcggcgga
     2881 tcgtcggcga cgtcccgcgg gtcgcctttc tctcctacag caccaagggc agcgcggagg
     2941 gcgtgcgcgt ggagaaggtg cggcgcgccg tagcgcggtt tcgcgagttc gcccccgacg
     3001 tggcgtgcga cggcgagctg cagggggacg cggcactcgt ggccgacgtc gcgcgccgca
     3061 aggcgccggg gtccccggtg gcgggacagg ccaacgtgct ggtgtttccc gatcttgaca
     3121 gcggcaacat cgcctacaaa ctggtgcagc ggctgggtgg gtgggacgcg ctcggcccga
     3181 ttctgcaggg tatcgcccgg ccgatcgcgg acctgtcgcg cggggcgagc gtggaggata
     3241 ttgtggacac ggcggcggtc acggttctcc aagcggaggg gtgagatggg gttctcgttg
     3301 agcaaggatc cgcggggtgt ggtgatcgtc ggcgtcgacg gccagctcat cgtcggcaac
     3361 cgccaggagc tcaagcagaa ggtgctcgac tccctggaag gcgggacgcg ccgcttcgtg
     3421 atcgacttca ccaagacggg gtacatcgac tcctccggcc tcggcgtgct cgtctcgctc
     3481 tcgaagaagg tgcgggagca gggcggggag ctgcggctcg cggggctcaa cgaagatctt
     3541 cagaccttgt tcgagctcac caagctggac acgctgttcg ccatcgccaa gacgccggcg
     3601 gaggcgctcg ctgccttcta gcgacgtccg cccgtgaacc accagctgca ggtcgtcgtc
     3661 caccggtctg cacgcgtggc aagtcccgga gccgacgtcg cggtgtcgct gtcggtcccc
     3721 agcgacctgg ggttcgtcgg cgacgccgtg gagctggcgg cccgctactg cgacaccgga
     3781 gtgctctccc cccgccggct gcaattcaac ctgcgcacgg ccctggccga ggcgctcgcc
     3841 aacgccatcg agtacggcaa ccggcgggac cccgccaaac gggtccgggt ggagatcgcg
     3901 tgcggccgcg aggcggtgcg gatcgacgtg gcggacgacg gcgccggatt cgatccggag
     3961 ggcgtgccgg atcccacggc cccggagaac ctggagcgtg agaccggccg cggactgttc
     4021 gtcctccgcc atctcgtcga ccaggtcgaa ttcaacgaac gagggaaccg cgtgtgtctc
     4081 gtcctgctgg cgggctgacc aaactcgagc gcctcctgcc ggcgctggag cagctgggag
     4141 gcgcgagcct cgcgctgcgc gtgtggcgcg ccgagggcgc cgccgtgcgg ttggtcaccg
     4201 ggacgccggc ggcgtggacg ccgtcgctcg gggagggcgc ggctcgcctc gacacgcccg
     4261 acggtcccgc ctggttcgtc ccgatgccgg cgccggggca gagccggggc gggcccaccc
     4321 tgtggctgga gctccgctcc ggcaaggagc tcacacccga gtcgcgcgag tcgctggtcg
     4381 aggcggtggc ctgtctcgcc gaagcggagc gtgaagcggg gcaggtggcg gcggagctgt
     4441 cggaccgcta cgaagagatc gacctcgtct acaccatcag tgagatcctc ggacacacca
     4501 tccgcctgga cgaggccgcc cgacgcatcc tgcaggaggt ggcgaccgtc gtcggcgcgc
     4561 ggcgcgccag tctgttcgtc ctcgacctcg accggcacgt gctgcggatc gtcgcctcca
     4621 agggcatggt cacccaggac gtggatccga tcgagctcga cgacccggtc tcggtgacgg
     4681 cccgggcgtt tcgcgaaatg cgcatccaga gcaatgaccc ggccgagccc gagccggcgg
     4741 cggcgcgggg acaaaccggg cggagttatc gggggcgcgc gttcctggcc gtgccggtga
     4801 tgtacggagc gccgggcgac caggcgcggc cgatcggcgt catcaatctc accgaccggc
     4861 acggtccgga cctgttctcc gcgggggacc gcaagctggt cgccgcgatc gcgaaccagg
     4921 tcggggcggc catcgagaac gcccggctcg tggcccgcga tctcgggcag cagcgggtgc
     4981 gccgtgagct cgagctcgcg cacgacctgc agctgaaact gttgccgtcc ccggcggtcc
     5041 tgggcgacaa ggccgatctg gcggcccgct gccggccggc cgattcggtg ggcggcgatt
     5101 tctacaatct gctccggctg cacggcgacc gcgtgggcat catgatcggc gacgtctcga
     5161 accacgggtt cggtgccgca ctgatcatgg cgctcgcgat ggccgcgtcg ggtattcatg
     5221 ccgaatcctc cgccgccccg gcgcaggtgc ttggtagcct cgagcagtcg ctcgccgccg
     5281 agctggcccg gacggagatg tacctgtcgc tc
//
LOCUS       DBNGZW010000522         3920 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_105589, whole genome shotgun sequence.
ACCESSION   DBNGZW010000522 DBNGZW010000000
VERSION     DBNGZW010000522.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_105589"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1753)
                     /locus_tag="AC16JC_12415"
     CDS             complement(<1..1753)
                     /locus_tag="AC16JC_12415"
                     /inference="COORDINATES: protein motif:HMM:NF025092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR5165544.1"
                     /translation="MAQRVEAGAPGPVRGVVLDAETGGPIQGVLVTARNAARGVTTDR
                     LGRFAIWIAQFPDTLTVVCIGRKPATVPIAAPPSDVVRISLETTPLVLSDVIVTAPAG
                     TTRPLEDVGRWQVPIASARGLPPAIEPDVFRALALVPAVSFSTPLSARPVIRGYDAGQ
                     SSVRIDGFEVLNLYHLGRIFSAFPADAASQITLATAPPSVVTGGTLAGTVDVSGQDGE
                     PGHATGGADLSLASLAGWGGGGGRAAQGFAAARAIHFSVINAFKTGTVPYDFQDLYAN
                     TLFSRQGRPAGRITAFASRDHLFDRDLGTGMDWSNLLFGIRWQAFDDGRHGASLWANA
                     NRFAEDAADVPARYTRLDLRNRFERIAAGVEGALQGPAARMALGFSAGTRSIANRITP
                     LSGVEFVATDTSVRRLEWSAYGELTGALGRASLQAGLRMDAAGGTHVWQPRARAAVFL
                     ATGASLGVAAGRTAMLYHLVSDLQSEPDLAFYDFWLGAGEGGVPVPVVDHGTVDLDLA
                     HGSFAGRVSLFASRGSGLAELRPASDQRAGVNAPFRYGRSRSRGLEAQLALRGDSARA
                     NALSVSYVLSWAQRDWDG"
     gene            complement(1786..2490)
                     /locus_tag="AC16JC_12420"
     CDS             complement(1786..2490)
                     /locus_tag="AC16JC_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165545.1"
                     /translation="MAFLALGLYACGAAIEPPPGGSVPVIQTLLVAGDSQQIAWVEWR
                     VHPDSSFGPDVRPVDPSLVQISLILPTGSSVPFTPSANIPGRFEAAVSVSPAMRYRLT
                     GTVAGFGLAAETTVPDTLAVRVPALDTVDGASCVDQFFYCEVPFIWSAAGATAYMYFQ
                     AQVDPVRLSRFGSTRDSTGVMLLYRNTGTERLTVLALEDNAAAFLTVRTLKSSVSGIF
                     GMFGAATRAERWIVWP"
     gene            2640..>3920
                     /locus_tag="AC16JC_12425"
     CDS             2640..>3920
                     /locus_tag="AC16JC_12425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015213526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR5165546.1"
                     /translation="MFTPPIRPFLALAALLATPSLTSSGPTGRIGGHVRDASGAPIAH
                     AQVLVVGTPLGAVTNVTGAYSIDSVPVGTYTVRAQFIGYTPAVLQNVQVRAGQAVSVD
                     LVLQPATMNLEAIVVTGRAAKVRGRQSLPATISGRAYADRMDEPYRVRREPWNTEEYG
                     HTEENPFLAVSAHPLSTFSIDVDRAAYSNVRRFLLDGQAPPKDAVRIEELVNYFPYDY
                     AGPTGDDPVAIHTELGPAPWKAQHQLLRIGLQARKVDLRGLPPSNLVFLLDVSGSMDE
                     PNKLPLVKASITMLVNNLRHEDRVAIVVYAGSAGLVLPSTQGDEKAKILEAIDRLEAG
                     GSTAGGAGIKRAYDEAVANFIRGGNNRVILATDGDFNVGVSSDGELVRLIEDRRKSGV
                     FLTVRGFGRGNLKDSKMEQLADHGNGNYAYIDNLL"
BASE COUNT          699 a         1379 c         1283 g          559 t
ORIGIN      
        1 tcccgtccca gtctcgctgc gcccaggaga gtacatagga tactgacagc gcattcgccc
       61 gcgccgagtc gccccgcagc gccagctgtg cctccagacc gcggctgcgc gatcggccat
      121 accggaacgg cgcattcact ccggcccgct gatcgcttgc cgggcgcagc tccgcgagac
      181 cgctgccgcg ggaggcaaac agcgacacgc gtcccgcgaa gctcccgtga gccaggtcga
      241 gatcgactgt cccgtgatcg actaccggca ccgggacgcc tccctccccg gctccgagcc
      301 agaagtcata gaaagccaga tcgggctcgg attggagatc cgagaccagg tgatagagca
      361 tcgccgtcct gcccgcggca acgccgaggg aagcacccgt ggccagaaaa acggccgcgc
      421 gcgcgcgggg ctgccatacg tgcgtcccgc ctgccgcgtc catccgcagg ccggcctgga
      481 gcgacgcgcg ccccagagcc cccgtcagtt ccccgtaggc gctccattcg aggcgccgaa
      541 cgctggtatc ggtcgccacg aactctaccc ctgacagcgg cgtgatcctg ttggcgatgg
      601 accgtgtccc ggccgagaat ccgagtgcca tccgtgccgc gggtccctgc agcgctccct
      661 cgaccccggc ggcgatccgc tcgaagcggt tgcgcagatc gagccgggtg tagcgcgctg
      721 ggacgtccgc agcgtcctcc gcaaaacggt ttgcgttcgc ccacagactc gcgccgtggc
      781 gcccgtcgtc gaacgcctgc caccggatgc cgaacagcag attgctccag tccatcccgg
      841 tgccgaggtc ccggtcgaag agatggtcgc gcgacgcgaa cgccgtgata cggccggccg
      901 gccgcccctg ccgggagaac agggtgttgg cgtagaggtc ctgaaagtcg tacgggacag
      961 tccctgtctt gaaggcattg atgaccgaga agtgaatggc ccgcgccgcg gcgaatccct
     1021 gggccgcacg accgcccccg cccccccagc ccgccagcga agcaagactc aggtcggcac
     1081 ctccggtcgc gtgccctggt tcaccgtcct gcccgctgac gtcgaccgtc ccagccagcg
     1141 tgcctcccgt tacgacgctc ggcggggccg tggcaagcgt gatctggctg gcggcatcgg
     1201 ccgggaacgc cgagaagatc cgcccgaggt ggtacaggtt cagcacctcg aagccatcga
     1261 tccgcacgct tgactgcccg gcgtcgtatc cgcgtatgac cggccgcgca gagagcggcg
     1321 ttgaaaaact caccgcgggc accaatgcga gcgcgcggaa tacatcgggc tcgatcgcgg
     1381 gcggcagccc tcgggcgctg gcgatcggca cctgccatcg tcccacgtcc tccaggggtc
     1441 gagtggtgcc ggcaggggcc gtcacgatca cgtcggacag cacgagcggc gtggtctcga
     1501 gggaaatacg taccacgtcc gacggcggcg ccgcgatggg aacggtggcg ggcttccggc
     1561 cgatgcagac cactgtgagg gtgtccggaa actgcgcgat ccagatcgcg aatcgaccga
     1621 ggcggtcggt ggtcacgccc cgggccgcat tgcgggcggt cacgagcacc ccctggatcg
     1681 gccctcccgt ctcggcgtcg aggactacgc cacgcacggg cccgggcgcg ccggcctcca
     1741 ctctctgagc cacgagggac agaaggaaag cgcccgccgc gctggtcacg gccagacgat
     1801 ccagcgttcc gcgcgtgtcg cggcgccgaa catcccgaag attccgctta cgctgctttt
     1861 tagcgtccgg acggtcagga aggcagcggc attgtcttcg agtgccagga cggtgagacg
     1921 ttccgttccc gtatttcgat acagcagcat gacgcccgtc gaatcacgtg ttgaaccaaa
     1981 tcgactcaga cgcacggggt ctacttgcgc ctggaagtac atgtaggcgg tggccccggc
     2041 cgcggaccaa atgaaaggaa cctcgcagta gaagaactga tcaacgcacg aggcgccgtc
     2101 caccgtgtcc agggcaggga cgcgcacggc gagcgtgtct ggaacggtcg tctcggcggc
     2161 aagcccgaat ccagcgaccg taccagtcaa tcggtatcgc atggcgggag acaccgacac
     2221 cgctgcttcg aagcgcccgg ggatgttggc tgacggcgtg aaaggaacgg agctgccggt
     2281 gggcaggatc agcgagatct gcacgagaga agggtccacc ggtcgaacgt ccggcccgaa
     2341 gctcgagtcc gggtgcacgc gccactcgac ccaggcgatc tgttgcgagt cgccggccac
     2401 gagcagggtt tgaatcaccg gcaccgagcc acctggaggt ggttcgatcg ccgcgccgca
     2461 ggcgtacagc ccgagggcga ggaatgccac agccgacgag aaacggaacc caggcctcac
     2521 ggcaggcaga agctccggtc tggatggttg agaactatga caaagtcgac tcgcccccat
     2581 cacctgtgca agatctcgac ggaagaccgt ctctctaggg cactcctact ggagagacca
     2641 tgttcacccc accgatccgg ccgttcctcg ctctcgccgc gctgctcgcg acgccgagcc
     2701 tcacctcctc cggtcccacc ggccggatcg gggggcacgt tcgcgacgcc tctggggcgc
     2761 ccatcgccca cgcccaggtg ctcgtggtgg gcacgccgct cggcgccgtc acgaacgtca
     2821 ccggcgcgta ctcgatcgat tccgtccccg tcggcaccta caccgtccgc gctcagttca
     2881 tcggatacac gccggccgtc ctccagaacg tccaggtgag ggcgggccag gcggtttccg
     2941 tggacctcgt gctgcaaccc gcgaccatga acctcgaggc gatcgtcgta accggccgcg
     3001 ctgccaaggt gcggggccgc caaagcctgc ccgccaccat ctccggcaga gcctacgccg
     3061 accggatgga cgaaccgtat cgcgtccgcc gtgaaccctg gaacaccgag gaatacgggc
     3121 acaccgagga gaatcccttc ctcgccgtct cggcccaccc gctctcgacc ttctccatcg
     3181 acgtggatcg cgcggcctac agcaacgtgc ggcgcttcct gctggacggt caggccccgc
     3241 ccaaggacgc ggtgcggatc gaagagctgg tgaactactt cccgtacgac tatgccggac
     3301 cgaccgggga cgacccggta gcgatccaca ccgagctggg accggcgccg tggaaggccc
     3361 agcaccagct gctgcgcatc gggctccagg cgcgcaaggt cgacctgcgc ggcctgccgc
     3421 cgtcgaacct ggtcttcctg ctggacgtgt cgggctcgat ggacgaaccg aacaagctgc
     3481 cgctggtcaa ggcgtcgatc acgatgctcg tcaacaacct ccggcacgag gaccgggtgg
     3541 cgatcgtggt gtacgccggc tccgcaggcc tcgtgctccc gtccacgcaa ggtgacgaga
     3601 aagcgaagat cctcgaggcg atagaccggc tcgaggcggg cggcagcacg gccggcggcg
     3661 ccggcatcaa gcgcgcctac gacgaagcgg tcgccaactt catccgcggc ggcaacaacc
     3721 gcgtgatcct cgccaccgac ggcgacttca acgtcggcgt ctcgagcgac ggcgagctgg
     3781 tccgcctgat cgaggacagg cgcaagagcg gcgtgttcct caccgtgcgc ggcttcggga
     3841 gggggaacct gaaggactcg aagatggagc agctcgccga ccacgggaac ggcaactacg
     3901 cctacatcga caacctgctc
//
LOCUS       DBNGZW010000523         2480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_718836, whole genome shotgun sequence.
ACCESSION   DBNGZW010000523 DBNGZW010000000
VERSION     DBNGZW010000523.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2480
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_718836"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            218..1855
                     /locus_tag="AC16JC_12430"
     CDS             218..1855
                     /locus_tag="AC16JC_12430"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HIR5165547.1"
                     /translation="MIFAWRLLGLPCAVVQVLAGGARTPDSSAARVDLARWLARSWPP
                     ITLAQPARMNDSLWRLSRRFYAGRSQLPAWRVAEGRGRLDELLDRLQRAQQQGLDSAD
                     YGLDRLRSLATRGDDSESAALLDLLATATWLHYASDLVYGRVIPRAVDSLWTASPRTT
                     VDLLDALAVALDSNRVGATLDDLAPPQRSALHLRAALAWYRAIAARGGWPQLPDGPPF
                     QLGAAGPTVAVLRQRLEITGDLAPGGLGTVFDSALDVAVRRAQQRHGLEPDGIVGGAT
                     RRALNVPVQDRIRQLELSLERWRWLPRDLGPRYLMVNSAGFSLELVDSGRVVLQSRIV
                     AGRVTWPTPIFSSRITEITFNPRWNIPRSIAVEEILPKLRRNPEHLTRTGIHLERVDE
                     PGNELACNEVQWDSVTAAGFEYRLWQEPGPENPLGRIRFTIPGPFGVALHSTQAPSLF
                     LLLVRASSHGCVRVEEAERLAAYLLQDRPGWTEDSLRAAIDTLVERYVAVPDPLPVYV
                     TYRTAWMSDGQVSFRPDLYRWDAELAAALRRRGRGLR"
     gene            1852..>2480
                     /locus_tag="AC16JC_12435"
     CDS             1852..>2480
                     /locus_tag="AC16JC_12435"
                     /inference="COORDINATES: protein motif:HMM:NF012350.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR5165548.1"
                     /translation="MTDKAFWHTSVAALCAELDATAEGLTASAAVQRLSIAGPNLLRI
                     HHEPTFVPLLLSRFRNPLVILLVAASVIAALTGDVASFVIIVLAVVLSVTLDTVQEHR
                     AGQAAERLRQSVAMRTIAVRDGQPTEVPAGDIAPGDVVILSAGDLVPADGRVLQARDC
                     FVNQALLTGEPYPAEKVAREEGEPDTQMAEARGALFMGTSVVSGTATMLV"
BASE COUNT          356 a          846 c          877 g          401 t
ORIGIN      
        1 gcgcggcgag cgaccaggtc acgaggatcg cgatcgcgaa ccagctcgca tcgagccgaa
       61 tggggatgcc acggaaactg aacagactta ggcgagggag cgccatatgc cctccgagcc
      121 cagcctaccc gggtccggct gagattcgcg tgaagcgcgc gttaggactt gatagaacgg
      181 ccccacgtcg ccggcggctc cgctaatttg ccggcagatg atcttcgcat ggcgattgct
      241 cggactgccg tgcgccgtgg tgcaggtgct ggcaggcggt gcgcggacgc cggattcgag
      301 cgcggcgcgt gtggatctcg cacggtggct cgcccggtcg tggcccccga tcacgctggc
      361 acaacctgcc cgcatgaacg attccctgtg gcggttgtcc cggcgcttct atgccgggcg
      421 ctcccagctg ccggcctggc gtgtcgccga agggcgcgga cgcctcgacg agctgctcga
      481 ccgcctgcag cgcgcgcagc agcagggact cgactcggcc gattacggac tcgaccggct
      541 tcgttcgctc gccacgaggg gcgacgattc ggagagtgcg gcactgctcg acctgctcgc
      601 caccgcgacg tggctgcact acgcctcgga tctcgtctac ggccgtgtga tccctcgagc
      661 ggtggacagc ctgtggaccg cgtcgccccg gacgaccgtg gacctgctgg acgcgctcgc
      721 cgtagcgctc gattcgaacc gtgtgggcgc gaccttggac gacctcgcgc cgccccagcg
      781 atccgcgctg catctgcgtg cggccctggc atggtaccgg gccatcgcgg cgcgcggcgg
      841 ctggccgcag ctacccgatg gcccgccgtt ccagctcggc gctgcaggcc ccaccgtggc
      901 ggtgctgcgc cagcggctcg agatcacggg cgatctcgca ccgggcgggc tgggaacggt
      961 gttcgacagc gcgctcgacg tggcggtgcg gcgcgcacag caacggcacg gactcgagcc
     1021 cgacgggatc gtgggtgggg cgacgcgacg ggcgctgaac gtcccggtcc aggaccggat
     1081 ccggcagctc gagttgagtc tggaacgctg gcgctggctg ccgcgcgacc tggggccgcg
     1141 ctacctgatg gtcaactcgg cagggttctc gctggagctc gtcgactccg ggcgcgtggt
     1201 gctccagagc cgcatcgtgg cgggtcgggt gacctggccg acgccgatct tcagttcccg
     1261 tatcaccgag atcaccttca atccgcgctg gaacatcccg cggagcatcg ccgtcgagga
     1321 aatcctgccg aagctgcggc gaaatccgga gcacctgact cgcacgggga ttcacctgga
     1381 acgtgtcgac gaacccggga atgagctggc gtgcaacgaa gtgcagtggg acagcgtgac
     1441 tgccgcgggg ttcgaatacc ggctgtggca ggaacccggt cccgagaatc ccctggggcg
     1501 catccgcttc accatacccg gtccgttcgg cgtcgccttg cactccacgc aggcgcccag
     1561 cctgttcctg ctgctcgtgc gggcgtccag tcacggctgc gtccgggtgg aggaagcgga
     1621 gcggctcgcc gcctacctgc tgcaagaccg ccccggctgg acggaggatt cgctgcgtgc
     1681 cgccatcgat actcttgtcg agcgatacgt ggccgtcccc gatccgctgc ccgtctatgt
     1741 gacctatcgg accgcgtgga tgagcgacgg ccaggtgtcg ttccgccccg acctctatcg
     1801 ctgggatgcc gagctggccg cggcgctgcg gcgccgcgga cgcggtctgc ggtgaccgac
     1861 aaagccttct ggcacacgtc cgtcgcggcg ttgtgcgccg agctcgatgc gacggcggaa
     1921 ggcctcaccg cgtcggccgc ggtccagcgg ctgagcatcg cgggcccgaa cctgctgcgc
     1981 atccaccacg agccgacgtt cgtgccgttg ttgttgtcac ggttccgcaa cccactcgtc
     2041 attctgctcg tcgcggccag cgtaatcgcc gccctcacgg gggacgtggc cagcttcgtg
     2101 atcatcgtcc tcgcggtcgt gctgagcgtc acgctcgata ccgtccagga gcaccgcgcc
     2161 gggcaagccg ctgagcggct gcggcagtcc gtggcgatgc ggacgatcgc cgtgcgtgat
     2221 gggcagccga cggaagtgcc cgccggcgac atcgcgccgg gggacgtggt catcctgtcc
     2281 gctggcgacc tcgtacccgc ggatgggcgg gtgctccagg cgcgcgattg cttcgtgaat
     2341 caagcgctgc tgaccggtga gccgtatccc gcggagaagg tcgcccggga agagggagag
     2401 ccggacactc agatggccga ggctcgcggc gctctgttca tgggcacgtc ggtggtgagc
     2461 ggcaccgcca ccatgctggt
//
LOCUS       DBNGZW010000524         3047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_186675, whole genome shotgun sequence.
ACCESSION   DBNGZW010000524 DBNGZW010000000
VERSION     DBNGZW010000524.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_186675"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..230)
                     /locus_tag="AC16JC_12440"
     CDS             complement(<1..230)
                     /locus_tag="AC16JC_12440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019958369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR5165549.1"
                     /translation="MIALARKYRPKRFADLIVQDHVAAALRGAVVQGRVAHGYLFAGP
                     RGVGKTTAARILAMALNCEKKSEGQGEPCGVC"
     gene            complement(335..1789)
                     /locus_tag="AC16JC_12445"
     CDS             complement(335..1789)
                     /locus_tag="AC16JC_12445"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012876120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5165550.1"
                     /translation="MNWELWVSRAIEGFNGFVLAYFLALNLSYIALFFVSLREVLTFL
                     QRTFFSDYQQILRSDMTLPITLIVAARNEEKTIVDTVRSLQMLNYPEFEIIVVNDGSK
                     DATLGRMLKAYDLRRVDRVYKRSLPTRNVLGLYASPLVPNLTVVDKEGGGKSDALNCG
                     INLSKYPLFCSLDADSIIEDDALLKAVKPFMEHPDETVAVGGIVRCANGCTVTEGRVT
                     RIALPGRFLPIFQVVEYLRAFLSGRLGWSALQGLLLISGAFGVYRKSAVVEVGGYDSS
                     TDTEDLELVLALHHVARAAKRRYRIVFVPDPVCWTEVPTTWQMLRRQRNRWHRGMLQS
                     LSRHRRMLFNPRFGMIGMVVLPYFLVFETMGPFVETLGYVSVPLAWVLGLLNTEFFVL
                     FFILAVAFGVFLSVAAILLEEISYRRYPSWEDLWKLLSCAVLENFGFRQLLALFKIQA
                     FWEFLRGLRRWGRLERVGFEKAVGEARVSVEVRR"
     gene            complement(1786..>3047)
                     /locus_tag="AC16JC_12450"
     CDS             complement(1786..>3047)
                     /locus_tag="AC16JC_12450"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR5165551.1"
                     /translation="VAPRAVTPATLALTVRWAIAVLGGMFGVVLLVTLGHKLLVQVRS
                     GYERRHRAMFLPRIQAYINGDHGRFREYVPGPLGRWDRRVVQWTLLEQIRYVRGKVQE
                     RISAAFEELGFVEEELGKLTHKRWTRRVEGAEKLGRMMSRRPLPDLTRLMQDPVPEVR
                     IRAAKALGAIGGLEAVESLLGALRDTNRWSALRISDILADLGEAAVGPLIREFPRLPP
                     LARVPAVDILGRLRSPTAVPLLVSLLRDPEDNARARAAHSLGLIGDPRAAPDLVLALG
                     DPAWPVRAMAAKALGILPGGEGIAALQGALADREWWVRSNAGEALRLKGEPGFRALVA
                     MLDGRDVYAAQKAASMLQEAGVFDGYVAQLVEGSLRERNAARQLLGKLVTLQRTDLLA
                     DVARRHADPRVRDAIGRLLEPARDIGA"
BASE COUNT          507 a         1089 c         1035 g          416 t
ORIGIN      
        1 tcgcacacac cgcacggctc gccctgcccc tcgctcttct tctcgcagtt gagcgccatg
       61 gcaaggatgc gcgcggcggt ggtcttcccc accccgcgcg ggccggcgaa gagatagccg
      121 tgggcgacgc gtccctgcac cacggcgccg cgcagcgcgg ccgcgacgtg gtcctggacg
      181 atcaggtcgg cgaaacgctt ggggcggtac ttgcgggcga gggcgatcat tggagctcga
      241 aggcgggcat cgagaaacaa tgtactggtg tcgggtgtcg ggtgtcaggt gtccaggtct
      301 ccgctgccga cacccggcac ctggcacccg acacctatcg tcgcacctcc accgacacgc
      361 gcgcctcgcc caccgccttc tcgaacccca cccgctccag ccgcccccag cgccgcagtc
      421 cccgcaggaa ctcccagaac gcctggatct tgaacagcgc cagcagctgg cggaatccga
      481 aattctcgag cacggcgcac gacaggagct tccacagatc ctcccacgac ggatagcgcc
      541 ggtaggagat ctcctcgagc aggatcgcgg ccaccgagag gaacacgccg aacgcgacgg
      601 cgaggatgaa gaacagcacg aagaactcgg tgttgagcag gcccagcacc cacgccagcg
      661 gcaccgagac gtagcccagc gtctccacga acggccccat cgtctcgaac acgaggaagt
      721 acggcagcac caccatgccg atcatgccga agcgcgggtt gaacagcatg cgtcggtgcc
      781 gcgaaaggct ctgcagcatg ccgcggtgcc agcgattgcg ctgccgccgc agcatctgcc
      841 aggtggtggg cacctcggtc cagcacacgg ggtcgggcac gaacacgatg cggtagcgcc
      901 gcttggccgc gcgggccacg tggtgcagcg ccagcaccag ctcgaggtcc tcagtgtcgg
      961 tgctggagtc gtagcccccg acctccacca ccgccgactt gcggtacacg ccgaacgcgc
     1021 cggagatgag cagcagcccc tgcagggcgc tccagcccag gcgtcccgac aggaacgccc
     1081 gcaggtactc gaccacctgg aagatgggga ggaagcgccc cggtagcgcg atgcgggtga
     1141 cgcgcccctc ggtgacggtg cagccgttgg cgcagcgcac gatgccgccc accgccacgg
     1201 tctcgtcggg gtgctccatg aacggcttca ccgccttgag caacgcgtcg tcttcgatga
     1261 tggaatcggc gtccagggag cagaacagcg gatacttgga gaggttgatg ccgcagttga
     1321 gcgcgtcgga cttgcctccg ccctccttgt ccaccaccgt caggttcggc accagcggcg
     1381 aggcgtacag gccgagcacg ttccgcgtgg ggaggctgcg cttgtacacg cggtccacgc
     1441 gccgcaggtc gtaggccttg agcatccgcc cgagcgtcgc gtccttcgag ccgtcgttca
     1501 cgacgatgat ctcgaactcc gggtagttga gcatctggag cgagcgcacc gtgtcgacga
     1561 tcgtcttctc ctcgttgcgc gccgccacga tgagcgtgat gggcagcgtc atgtccgagc
     1621 gcaggatctg ctggtagtcg gagaagaagg tacgctgcag gaaggtgagg acctcgcgca
     1681 acgacacaaa gaacagcgcg atgtaggaga ggttgagcgc caggaagtag gcgaggacga
     1741 agccgttgaa cccctcgatc gcccgactga cccacaactc ccagttcatg ctccgatgtc
     1801 gcgcgcgggc tcgagcagtc tgccgatggc gtcccgcacg cgcgggtcgg cgtggcgccg
     1861 cgccacgtcg gcgagcaggt cggtgcgctg cagcgtcacg agcttgccga ggagctggcg
     1921 ggcggcgttg cgctcgcgca ggctcccctc cacgagctgg gcgacgtacc cgtcgaacac
     1981 gccggcctcc tgcagcatcg aggcggcctt ctgggcggcg taaacgtccc ggccgtccag
     2041 catggcgacg agcgcccgga acccgggctc acccttgagg cgcagcgctt cgccggcgtt
     2101 ggagcgcacc caccactcgc ggtcggcgag cgcgccctgc agcgccgcga tgccctcgcc
     2161 gccgggcaga atgccgagcg ccttcgccgc catggcgcgc accggccagg ccgggtcgcc
     2221 gagcgccagg acgagatcgg gggcggcgcg cggatcgccg atcaggccga gtgagtgcgc
     2281 cgcccgggcc cgcgcgttgt cctcgggatc gcgcagcagc gagacgagca gcggcacggc
     2341 ggtgggcgag cgcagccgtc ccaggatgtc gacggcgggt acgcgggcga gcggcgggag
     2401 gcgggggaat tcgcggatca gcggccccac cgccgcctcg cccaggtccg ccaggatgtc
     2461 gctgatgcgc agcgccgacc agcggttggt gtcgcgcagc gcgccgagca gcgactccac
     2521 cgcctccagc ccgccgatgg cgcccagcgc cttggcggcg cggatgcgca cctccggcac
     2581 ggggtcctgc atcaggcgcg tcaggtcggg cagcggccgc cggctcatca tgcgccccag
     2641 cttctcggcc ccttccacgc ggcgggtcca gcgcttgtgg gtcagcttgc ccagctcttc
     2701 ctccacgaag cccagctcct cgaacgcggc gctgatgcgc tcctgcacct tgccccgcac
     2761 atagcgtatc tgctccagca gggtccactg taccacgcgc cgatcccagc gtcccagcgg
     2821 accaggcacg tactcgcgga accggccgtg gtcgccgttg atgtaggcct gaatgcgcgg
     2881 caggaacatg gcccggtgac gccgctcgta gcccgagcga acctggacca gcagcttgtg
     2941 gccgagcgtc accagcagca ccacaccgaa catccccccc aggacggcga tcgcccagcg
     3001 aaccgtcagc gccagtgtcg cgggggtcac ggctctgggc gcgacct
//
LOCUS       DBNGZW010000525         3480 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1741254, whole genome shotgun sequence.
ACCESSION   DBNGZW010000525 DBNGZW010000000
VERSION     DBNGZW010000525.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3480)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3480)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3480
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1741254"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1425)
                     /locus_tag="AC16JC_12455"
     CDS             complement(<1..1425)
                     /locus_tag="AC16JC_12455"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003387217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR5165552.1"
                     /translation="MSDRRPVYGPDDWAGDPARQPGEPGAFPFTRGVHPTMYRGRLWT
                     MRQYAGFGTAEETNARFRHLLAAGQTGLSVAFDLPTQMGYDSDHPMAEGEVGRAGVAI
                     DTVDDLERLFHEIPLDKVSTSMTINATAAILLAMYVVVAEEQGVPRNALQGTIQNDIL
                     KEFIARGTYIYPPEPSLRLIVDVFRYVADEGMKFNPISISGYHIREAGATAVQELAFT
                     FADGLEYVRRAVAAGLDVNRFAPRLSFFFAGYTDLFEEVAKFRAARRLWARLLRDRFG
                     AGEEACRLRFHTQTGGATLTAQQPVNNVVRVAIQALAAVLGGTQSLHTNSYDEALALP
                     TQASATLALRTQQVLAHETGVPQVVDPVGGSWYVERLTDELEGAAAALIDQVEALGGA
                     ARAIERGYFQNAIARSAYEQQRRVESGEQVVVGVNQFDDGSGIPNVPQPDYGALAEGQ
                     RRRIAEARARREGPAVARALARLRD"
     gene            complement(1422..2441)
                     /gene="fbp"
                     /locus_tag="AC16JC_12460"
     CDS             complement(1422..2441)
                     /gene="fbp"
                     /locus_tag="AC16JC_12460"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HIR5165553.1"
                     /translation="MHSSTSVVTIERFIMEQERQFPEATGELSNLLYDVCLAAKIISR
                     NVRRAGLSDILGEMGTTNVQGEMQQNLDVFANDTIRNAVQHTGRTCVLASEEDEEPVP
                     TPVGRPAGKYVLLFDPLDGSSNIDVNVSIGTIFSIHRRVTPDGGPGTLEDCLQPGHRQ
                     VAAGYILYGSSTMLVYTTGKGVHGFTLEPSIGEFLLSHPEFRTPEVGKYYSVNESYWD
                     RWSDTVHKTVAAFKSGTHGATAKNARYIGSLVADFHRNLVTGGIFLYPADTKNPQGKL
                     RLLYEAAPLALVAQQAGGLATDGHRPILDIVPGTLHQRVPLVIGSRKDVELATGILSQ
                     EPVRT"
     gene            complement(2410..>3480)
                     /locus_tag="AC16JC_12465"
     CDS             complement(2410..>3480)
                     /locus_tag="AC16JC_12465"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR5165554.1"
                     /translation="GLSSLVPALGALLAAGAGATLALLDLVAATAARVPGGHVISMPG
                     WPAGIAWTAVLAVAWWLWTSPRRPWLVAGRAAFVAAALAWGSLLARAPRDSDGVLTVH
                     FLDVGQGDAAALRTPAGHWVLIDGGPRTPQGDAGRRVVLPFLRRFGAGALALVVATHG
                     DADHLGGLPAVVEALPPRFVLEPGEPLGRPLYLEFLADVERAGATWHPARTGDRVELD
                     GVAFQVLSPDSGWAAQQEDVNEQSVVLLVTYGATRLLFMGDAGVPVEERLARRVGHVD
                     LLKVGHHGSRTATSDEWLSELAPAEAVISVGAHNTYGHPAPEVLARLLQHHVTTLRTD
                     QLGTITFTTDGHRALIDFRRHH"
BASE COUNT          527 a         1252 c         1195 g          506 t
ORIGIN      
        1 atcgcggagc cgcgcgagcg cacgtgcaac ggccggccct tcgcgccgcg cccgcgcttc
       61 cgcgatccgg cgtcgctgtc cttccgccag cgcgccataa tcgggctggg gcacattcgg
      121 gatgcccgag ccatcgtcga actggttcac cccgactacc acctgctccc ccgactcgac
      181 tcgccgttgt tgctcgtagg cgctgcgcgc gatggcattc tggaaatagc cgcgctcgat
      241 cgcgcgcgcc gccccgccca gcgcctcgac ctggtcgatc agcgccgcgg cggcgccctc
      301 cagctcgtcg gtgaggcgct ccacgtacca gctgcccccc accggatcca ccacctgcgg
      361 cacgccggtc tcgtgcgcca gcacctgctg ggtccgcagc gcgagcgtcg cgctcgcctg
      421 cgtcggcagc gccagtgcct cgtcgtagga gttggtgtgg agcgactggg tcccgccgag
      481 cacggcggcc agcgcctgaa tcgccacgcg aaccacgttg ttcaccggct gctgggcggt
      541 gagggtcgcc ccgccggtct gcgtgtggaa gcgcaaccgg catgcctcct cccccgcgcc
      601 gaagcggtcc cgcagcaggc gcgcccagag gcggcgcgcg gcacggaatt tggcgacttc
      661 ctcgaacagg tcggtgtagc ccgcgaagaa gaacgacagg cgcggcgcga accggttcac
      721 atcgagtccc gccgccacgg cgcgccgcac gtactccagg ccgtcggcga aggtgaaggc
      781 cagctcctgc acggccgtgg cgcccgcttc gcggatgtgg taccccgaga tcgaaatggg
      841 attgaacttc atcccttcgt cggccacgta gcggaacaca tcgacgatca gccgcagcga
      901 aggctcgggc gggtagatgt aggtgccgcg cgcgatgaat tccttcagga tgtcgttctg
      961 gatcgtgccc tggagggcgt tgcgcggcac gccctgctcc tctgccacga ccacgtacat
     1021 ggcgagcagg atcgccgccg tggcgttgat cgtcatggac gtggagacct tgtcgagcgg
     1081 gatctcgtgg aacagccgct cgagatcgtc cacggtgtcg atcgccacgc cggcgcgtcc
     1141 cacctcccct tccgccatgg ggtggtcgga gtcgtagccc atctgcgtgg gcagatcgaa
     1201 cgcgacggag aggccggtct gccccgccgc gagcaggtgg cggaagcggg cgttcgtctc
     1261 ttcagcggtg ccgaacccgg cgtactggcg catcgtccac aggcggccac ggtacatcgt
     1321 ggggtggacc ccgcgagtga acgggaaggc acccggctcg cccggctgcc gggcgggatc
     1381 ccccgcccag tcatccgggc cgtagaccgg gcggcggtcg ctcacgtccg gacgggctcc
     1441 tgcgagagga tgccggtggc cagctccacg tccttgcggc tgccgatcac gagcggcacg
     1501 cgctggtgca gggtcccggg gacgatgtcg agaatgggac ggtgcccgtc ggtggccagc
     1561 ccgcccgcct gctgcgcgac cagcgcgagc ggcgcggcct cgtagagcag gcgcagcttt
     1621 ccctgggggt tcttcgtgtc ggccggataa agaaagatgc cgcccgtcac gaggttccgg
     1681 tggaagtcgg cgacgagcga tccgatgtag cgcgcgttct tcgccgtcgc gccgtgcgta
     1741 cccgacttga aggcggccac cgtcttgtgc accgtatccg accagcggtc ccagtagctc
     1801 tcgttcacgc tgtagtactt gccgacctcc ggcgtccgga actccggatg cgacaacagg
     1861 aattcgccga tcgagggctc gagggtgaag ccgtgaacgc ccttgccggt ggtgtacacg
     1921 agcatcgtgc tcgagccgta caggatgtag ccggcggcga cctgacggtg ccccggctgc
     1981 aggcagtcct cgagcgtccc gggaccgccg tccggcgtga cgcggcggtg aatggagaag
     2041 atcgtcccga tggacacgtt cacatcgatg ttgctcgagc cgtcgagcgg atcgaacagg
     2101 aggacgtatt tgcccgccgg ccggcccacg ggcgtcggca ccggctcctc gtcctcctcc
     2161 gaggcgagca cgcaggtgcg cccggtgtgc tgcacggcgt tccggatggt gtcgttggcg
     2221 aagacgtcga ggttctgctg catctcgccc tgcacgttgg tcgtgcccat ctcgcccagg
     2281 atgtcgctga gccccgcgcg gcggacgttg cgggagatga tcttggccgc gaggcagacg
     2341 tcgtagagca ggttcgacag ctctcccgta gcctcgggga actgccgctc ctgctccatg
     2401 atgaaccgct caatggtgac gacggaagtc gatgagtgca cggtggccgt cggtggtgaa
     2461 agtgatggta cccagctggt cggtccgcag cgtcgtcacg tggtgttgca gaagtcgggc
     2521 cagcacctcc ggcgccgggt ggccatacgt gttgtgcgcc cccacactga tcaccgcctc
     2581 ggccggagcc agctcgctca gccactcgtc ggaggtcgcc gtgcggctgc cgtggtgccc
     2641 caccttgagc aggtccacat gccccacccg gcgcgccagc cgctcctcca ccggcactcc
     2701 ggcatcgccc atgaacagca ggcgcgtcgc cccataggtc accagcaaca cgacgctctg
     2761 ctcgttcacg tcctcctgtt gggcggccca tccgctgtcg ggactcagta cctgaaacgc
     2821 cacgccgtcc agctccacgc ggtcaccggt ccgggcggga tgccaggtcg cgccggcgcg
     2881 ctcgacatcg gcgaggaact ccagatacag cggccgcccc agcggctccc ccggctcgag
     2941 cacgaaccgg ggcggcaacg cttcgaccac ggcgggcaac ccgcccagat gatccgcgtc
     3001 gccgtgcgta gccacgacca gagccagcgc gccggcgcca aaccgccgca agaacggcag
     3061 gaccacccgc cgcccggcat cgccctgcgg cgtgcgcggg cccccatcga tcaagaccca
     3121 atgcccggcc ggggtgcgca gcgctgcggc gtcgccctgc ccgacgtcga gaaaatgtac
     3181 ggtgaggacg ccgtcgctgt cacggggcgc gcgcgcaagg agcgagcccc aggcgagcgc
     3241 ggcggcgacg aacgcagccc ggcccgccac cagccaaggc cggcgcggcg aagtccacag
     3301 ccaccaggcg acggccagca ccgccgtcca cgcgatcccc gccggccatc ccggcatcga
     3361 gatcacatgg ccgccgggca cgcgcgccgc agtggcggcc accagatcga gcagcgcgag
     3421 cgtcgcgcct gcgccggcgg cgagcagggc gccgagggcg ggcacgagac tcgacaggcc
//
LOCUS       DBNGZW010000526        18166 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_186793, whole genome shotgun sequence.
ACCESSION   DBNGZW010000526 DBNGZW010000000
VERSION     DBNGZW010000526.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 18166)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 18166)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18166
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_186793"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="AC16JC_12470"
     CDS             complement(<1..290)
                     /locus_tag="AC16JC_12470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019184827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HIR5165555.1"
                     /translation="MTTLRAVLAKLRLWFDTAATPAGGNPAEHRVDWVRVLPFVAMHL
                     ACLGVIWVGASWEAVGVAAGLYALRMFFVTAFYHRYFSHRTFETSRPAQFAFA"
     gene            414..1532
                     /locus_tag="AC16JC_12475"
     CDS             414..1532
                     /locus_tag="AC16JC_12475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165556.1"
                     /translation="MSRVPFAAAFTVALAGTLPAGLLAQTDSGQAPPPRGRAGYRALN
                     LGVHGVGLSIGNSRRWAGLRINFQDNAVERVDGLNLTIWNAKHNETMELNGVALGLAG
                     PVGGTFNGVSIGVIGAVAERGFHGVTLAGLGAVSNGDTRGLTVAGLGVVSNGDMTGVN
                     VSGLGTVANRSMRGLNLAGLGVVADGDMAGINVSGLGTVANGVLGGVSVGGLAVVGNR
                     GVWGIATGGLAVVSDEAVDGVSAAGLAVVGNGRVRGIAAAGLAVVTDQTMTGLAVAGL
                     AVVGGQGVRGVAIGGVHVEGRWIEGLAISPIRTRTWDLTGVSIAGYNRIKGEQHGLTI
                     GLYNYAKQLHGVQLGLINNARNNHGLLRVLPLVNLHLR"
     gene            1594..2565
                     /locus_tag="AC16JC_12480"
     CDS             1594..2565
                     /locus_tag="AC16JC_12480"
                     /inference="COORDINATES: protein motif:HMM:NF016816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HIR5165557.1"
                     /translation="MPRLGLLAVLLCAPCSLLAAQASPYTPPHLRAPQGFRVFIVPDM
                     EGMGSAVDIHEVIAGNEGEQYRTLTSPDYWDHFRGLLTREVNAAIAGARLAGARSFVV
                     NEGHGGNLFANILPWDLDTGAILIRGFPKPLVMVTGIDSTVGTLMFTGAHAGPGHPGI
                     LAHNFAFDTFTVNGKSLNEVGINALIASEYGVAVSLVSGDDAEIAETRAILGSGFIGI
                     VTKYAVGRMAAITFSPTQVRRWLRDSAAVAVRRAMRGDFKPFRLDKPYHVAFALRRSY
                     PDVVVARVDSLNWTGLEKTGERSYRLTTDDARQMGYLLDAIERVVLR"
     gene            2562..3608
                     /locus_tag="AC16JC_12485"
     CDS             2562..3608
                     /locus_tag="AC16JC_12485"
                     /inference="COORDINATES: protein motif:HMM:NF016816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HIR5165558.1"
                     /translation="MSARPLRMLALAALPALLAAQQPPQAQPPQQPPRDTTPPEAYYG
                     VTEYQQARSKLAALMQPTGFTVYIIADMEGLAGVVRNATEMRPEARGGHDPTHEAFRK
                     ELADEVNAAIAGARLAGATQFVVNEGHGGTLFRNIHPEDLDPAAILVRGYPKPNVMET
                     GLNPNVDALFIIGAHANAGTPGIIAHSYAFDSFTVNGHVLNEAGIAAFIGGEMGVPLA
                     LAAGDNVLTAETRGMVGELETVTTKIAESRSAAAVFPPAMVQRQIRAAAARAVRGVRT
                     GRIKPLHIERPYTVAFCTRPSFQPWVPEATARLAGVERIAGDSTGRCFTYHTDSAEAV
                     GTLLNEIEWIVLKP"
     gene            3661..5733
                     /locus_tag="AC16JC_12490"
     CDS             3661..5733
                     /locus_tag="AC16JC_12490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5165559.1"
                     /translation="MSRSLRFMLGIVAGLAAAPLAAQETASDTLLTINHYLDWEQVSD
                     PQLSPDGSQIVYTRTWVNRLEDRWESALWIMNADGTKARFLTKGSSARWSPDGSRIAY
                     LADGEPKGTQVFVRWMDAEGATSQITRVTESPGELRWAPDGKSLSFAMLVRHTTPWTI
                     SMPAAPTGASWTAAPRVVDRMHYRQDRRGFMEDGYLHLFLVPADGGTPRQLTSGDWNV
                     GARFDGLSSGVGYDWSPDGKSIVVDWFKDAGADRNYRGSRLYVVDVASGAIRQLTTRP
                     GTWNDPVVSPDGRSVAFTGYDSADVSYRAEDLWVIGLDGGGMRKVSGDLDRDPGGLRW
                     APDGNGLYFAAGDRGTQNVWFAALAGGGPRQVTSGTHMLSLTSLDRNLNAVGTQSDPS
                     NPADVVRYSLRRPGPATVLTGVNADVLGRIHLAKVEEVWYGSSGGARIQGWIVKPPSF
                     DPSRKYPLILEIHGGPHGMYGVGFNYMFQNFAADGYVVLYVNPRGSTGYGSAFGNAIF
                     HAYPSVDYDDLMAGVDTVIGRGFVDTTRMYVSGCSGGGVLSSWVIGHTHRFAAAAVRC
                     PVIDWISMAGQTDIPLFTYNFFDAPFWEKPDQWLKQSSLMYVGNVTTPTLLMTGELDL
                     RTPMPQTEEFYSALKYRGVEAVMLRFNGEYHGTGSKPTNFMRTQLYMMSWFQKHGGQQ
                     AAAASSSQ"
     gene            5730..6251
                     /locus_tag="AC16JC_12495"
     CDS             5730..6251
                     /locus_tag="AC16JC_12495"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5165560.1"
                     /translation="MTASLPEAWLRGPVEGIAPVLQPSAHALIQASEELGAAVTGLPV
                     AAVWAAPGGAASIGFHLRHIAASTDRLLTYARGEPLTAEQLRALAEEKMPGTPPVEGP
                     ALLARAQERVRAALDAYRGVDEGILFEPRAVGRKRLPTTVFGLLAHIGEHAMRHTGQV
                     VTTARIVRALSPD"
     gene            complement(6248..8356)
                     /locus_tag="AC16JC_12500"
     CDS             complement(6248..8356)
                     /locus_tag="AC16JC_12500"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR5165561.1"
                     /translation="MPDLEPILRPRSVAVIGASRRPDTIGNEILANIVRYGFTGAVFP
                     VNPKASNVHSIRAYPSVLEIPDPVDMAVIVVPREHVPTVAEQCGTKRVRGLVVISAGF
                     KEVGEAGAAAERQLVEIVHRHRMRMVGPNCMGVLNADPAVSLNATFAPSMPPFGAAAF
                     VSQSGALGMSVLDYAREYGIGISQFVSVGNKPDVSGNDLLLHWEHDDAVKVILMYVEN
                     FGNPRRFLEIASRIAKQKPIIALKSGRSKVGARAAASHTGALAASDAAVDALLTQAGV
                     LRATSMEELFDMAMAFNGPPLPRSRRTAVLTNSGGPGILIADALEPQGLELVDLTPET
                     IERLRPLFPPEASLRNPVDMIASATAADYRAALDAVLADGHVDSAVAIFVPPLGIRSE
                     DVAESIAAAARGHPSKPVLAVLMGRAGLQEGKAELHRAGIPTYIFPESAARALGALRR
                     VRGWRARPETAYKPLPVDHDGAARLLAQARSAGRQKLSELEVLGVLTAYGIPTAPARL
                     ARDPQDAAHAATEIGYPVVLKVVSPQIVHKTEVGGVRINLTTAVEVQEACAEMLNGVA
                     RAAPQARIDGILVQRMVPGGRETIVGVSRDRAFGPLVMFGLGGIYVEALRDVVFRIPP
                     IGPLEAHDMLKGIKSVAILDGVRGAPPVDFAALGEALQRVAQLATDFVEIVELDINPL
                     LAFPHGVTAVDARILLAPAS"
     gene            complement(8511..8729)
                     /locus_tag="AC16JC_12505"
     CDS             complement(8511..8729)
                     /locus_tag="AC16JC_12505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165562.1"
                     /translation="MTEVFDRAGAAPPLLAALRSALAPLRNLGDVLAWARTLDPPVTA
                     PAVVTQDEYTHDVLVPWRDGLTLAFDVT"
     gene            complement(8726..10462)
                     /locus_tag="AC16JC_12510"
     CDS             complement(8726..10462)
                     /locus_tag="AC16JC_12510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR5165563.1"
                     /translation="MACLIGGVGALVALGLLRLIGFFTHVFYYHEVASTLASPADAHP
                     GLWAVSIPVLGGLIVGLMARYGSERIRGHGIPEALEAILIGGSRMDVKVAVLKPLSSA
                     IAIGTGGPFGAEGPIIMTGGAFGSLFAQAFHLSPAERKTLLVAGAAAGMAAVFATPLA
                     AVLLAVELLLFEWKPQSFIPVAAAAAVAALLRVPLLGSGPIFPVTPHATLSWVVLACA
                     AGMGIAAGLGSGLLTALVYGAEDLFRKLPIHWMWWPMLGGLVIGVGGWLYPRALGVGY
                     DTIHDLLAGHQVGALLLGLLVTKAVIWAVALGSGTSGGVLAPLLIMGGAMGALLSPLL
                     PGADAGLWAGIGMGAMMGGTMRSPFTAIAFMLELTHDINVLPGLLIACVAADAVTVLL
                     MKRSILTEKVARRGHHVMREYSVSPLHRLWVEDVMERDVPAIPANLPLDTLFLQLAHL
                     DPVVGRRQAWPIVDEGGRLVGLITRGDLTRTVWQPDTAGRTVLQSGTKDIVVAHPDEL
                     LEVATARMVDHDIGQLPVVSRGEPGRVVGFLDRGAVMAAWTHAAREERAREEGWLAAP
                     FRMLRERLGGTG"
     gene            10648..11244
                     /locus_tag="AC16JC_12515"
     CDS             10648..11244
                     /locus_tag="AC16JC_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009154833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5165564.1"
                     /translation="MGKGDLTRQAILGRATGLASTAGLEGVTIGALAAELQLSKSGLF
                     AHFHSKEALQLQVLEFAAAQFVEHVIRPALAEPRGEPRLRALFERWLDWSHSSPLPGG
                     CLFVQASVELDDRPGSVRERLVGIQRQWLGVLATSLQKGIEAGAFHDGVDPEQFAQDV
                     YGVMLAYHHASRLLHDPRAETRARGAFETLLSAAGVAH"
     gene            11263..12177
                     /locus_tag="AC16JC_12520"
     CDS             11263..12177
                     /locus_tag="AC16JC_12520"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5165565.1"
                     /translation="MRLLRLGFRSLQAVSPSRAAALAERLFFTPPRSRLTPDMRAELE
                     RGRPFTVQVEGHRVACWSWGSTGLPIAYLVHGWGSRGGRLTAYVRPLQAAGFRVIAFD
                     GIGHGASEGQLSSAPQLARTLSALADAVGPAHSVVAHSLGAVVTTLAMEWGLPVSRAA
                     FLAPNADPIAHTLRWAHRLGMRPDVVAQMRAATERRIAFSWDDLDVVTMARRRTTPLL
                     VIHDATDEVVPWMEGAAIVGAWRGSRFVTTRGLGHSPLVRNPEVVTQVVEFLGTPEGS
                     RARPARVSGIEHELFYREWRPSLPPLSA"
     gene            complement(12169..13680)
                     /locus_tag="AC16JC_12525"
     CDS             complement(12169..13680)
                     /locus_tag="AC16JC_12525"
                     /inference="COORDINATES: protein motif:HMM:NF012935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5165566.1"
                     /translation="MTPASRSSDVVIVGSGAGGGTVAQLLAPLAQAGRRVLVLEQGPR
                     FGDDEFTGRELEMASALYHDGGGFLSADGTMTLAFAHGYGGSTIVYTGTSLIAPARVI
                     ERWAVPGLSHADVLRRSQRYLGENNVHRLPLEELNDNNRLFEAGARKAGYAVEQFPVN
                     VKGCRGSSLCNLGCPNQAKQGTHRVQLPAAERAGVEVVTRAEVLRITPDRSLTVRVSE
                     LLAGAKGRPSEWPAGEYRVEAKIVVCAAGALGSPPLLLRSPLPTPRSLGEGFTCHPAL
                     ILVAEHAVPITNDVGHPKSFFVDRAAEERFVLETCMYFPFTTAKALNGFGPEHSALMR
                     AFPRQQQILVLGIDRAADGNRVAIDRDGNPVVHYAYSRETVTGLVRGNRAAARIFFAA
                     GAVRVHAPFAEPPTIEAHDAGRLDTLIDARHWKPGKQAISSAHLMGGCRMGTSAADSV
                     TDAWGRVHGIPWLFVADASLFPDALEINPYVTIMALADRVAEAVLSDAPQLLG"
     gene            complement(13677..14078)
                     /locus_tag="AC16JC_12530"
     CDS             complement(13677..14078)
                     /locus_tag="AC16JC_12530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165567.1"
                     /translation="MLTSVRPVLRAVGVTVVPEATRLDEAGWAALERTVAAALAARPA
                     ALQRQFLLFLRLMEWLPVVRFGRRFTRLDAARRTRFLKSLQDAPLLLVRRGTWGLRTL
                     LLMGYYGRPEARDEIGYRAHPGGWTARRRVS"
     gene            14135..14716
                     /locus_tag="AC16JC_12535"
     CDS             14135..14716
                     /locus_tag="AC16JC_12535"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5165568.1"
                     /translation="MSERADEFGGLEAACGQFESTKQEAAELVAPLTDAQVNWCPAPG
                     RWSIGECLLHLATSTDAALPALDLAIEQAQIRGWTACGPYRYGWFTRWNVRSMEPPPA
                     FRVRTFRIFEPPTTAMRGGAVARALAESRDRLLERTRVAAGVDLKRAVVVSPALRLFR
                     LPLGGYLAFLAAHDRRHLWQARQVLRASGFGQA"
     gene            14838..15326
                     /locus_tag="AC16JC_12540"
     CDS             14838..15326
                     /locus_tag="AC16JC_12540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165569.1"
                     /translation="MLPAVVVAGTLLGCGASQSPVPVVGREVDVSQLAGEWSGDYSSV
                     ESGRSGSIVFKLVAGADSAMGEVIMTPQWLARPGGANTQPGPTTQPVSQPLTISFVRV
                     SGGQVSGALQPYPDPNCGCTLHTTFVGRLQGDTLQGSYSSVHEQTRDVQQGRWRVVRK
                     QP"
     gene            15350..17986
                     /locus_tag="AC16JC_12545"
     CDS             15350..17986
                     /locus_tag="AC16JC_12545"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HIR5165570.1"
                     /translation="MPAIAGLAVARLSAQGPAPAGPRPYDEPPHKQATAERVPPGAVR
                     MDGRLDDSVWLAARWIDDFVEKMPVEGGTPTDSMRLAIVYDDHALYVGARMYSHDPAH
                     IQAPLSRRDNTSQAEHLWVSFDTYHDRRTAYSFGVTATGVRMDWYHPRDHEYDIDPTF
                     DPVWEAKAVIDSLGWTAEMRIPFSQLRFNRGDAQVWGFNADHWIPSRNEDVFWIPVPQ
                     NRTGWSSWMGDLQGIRDIAPSRRLELLPYTAGSATLTGTRDAADPFDDGRNLKGRFGT
                     DLKMGLGPNLTLDATVNPDFGQVEADPAVVNLTAFEVFFDEKRPFFLEGSHLLRGDGP
                     SYFYSRRIGAPPRGSAAGDFVDYPSNSTIIAAGKVTGQTAAGFSVGTLAAVNAREFAR
                     TYDTATATFTRLQVAPPTAYGVVRAQQQFGATASTIGFTLTAVRRDFAPGSALDSLLD
                     RQAFTGGVDGVIRWAGGVYRLSGYAGFSAVRGDAPALIGIQSSPAHFYQRPDAGYIRL
                     DSTRTALNGYTLGLDFGKTGGGHWRYDAGFGVESPGFELNDAGRLFNADGRTGLASLN
                     YRETHPGRWYQSFGLGISGNAEWDFGGIRQFAVTQQNADLTFKNYWSVNAYLYQSWPS
                     LDHALTRGGPLMATPYSWGSGVRIGNGFGAKTSWNVRVSYGTDALGGEASQLSGRLSI
                     RPANRLELSISPRYAREISSRQYIATLDSGGPATYGQRYLFGAIDRSTLSAQLRLTYT
                     LTPDLTLELYAEPFAASGRYYGIGELARGRTTDLTLYSGDTTVVPSLIRNPAGEYTLK
                     DAGGYRRDLGNPDFNVLSFRSNLVLRWEWRPGSTLFLVWQQSRFALDTRGALVGPGAL
                     WDSFRASGDNFLALKVSYWLAP"
BASE COUNT         2801 a         6283 c         6325 g         2757 t
ORIGIN      
        1 gcgaaggcga attgggccgg gcgtgaggtc tcgaaggtcc ggtgggagaa gtaccgatgg
       61 tagaatgccg tgacgaagaa catgcgcagg gcgtagagac cggccgcgac gcccaccgcc
      121 tcccagcttg cccccaccca gatcaccccc aggcaggcga ggtgcatggc gacgaacggc
      181 agcacgcgca cccagtccac ccggtgctcg gctggattcc ctccggcagg tgtcgccgcg
      241 gtgtcgaacc acagccgcag cttggcgagt accgcccgta gcgtggtcat ggcgcgctca
      301 tgtaaacgtc gtctcgcctg cgcggccttg caagatgcac gccgacccgc catgtatgga
      361 accggacccg tcgcggtccc gtactagccg catcccaccg cccgaggacg ccgatgagcc
      421 gggtcccatt cgctgccgcg ttcaccgtcg cgctggcggg caccctgccc gccggccttc
      481 tcgcacagac cgactccggg caggcccccc ctccacgcgg tcgcgccggg tatcgcgccc
      541 tcaacctcgg cgtccacggc gtggggctga gcatcgggaa ctcgcgtcgc tgggccggct
      601 tgcggatcaa cttccaggac aacgccgtcg aacgggtgga cggcctcaac ctcacgatct
      661 ggaacgcgaa gcacaacgaa acgatggagt tgaacggcgt cgccctggga ctcgcgggcc
      721 ccgtgggtgg gaccttcaac ggcgtgagca tcggagtgat cggggcggtg gcggagcggg
      781 gctttcacgg ggtgaccctc gccggcctgg gagcggtttc caacggcgac actcgcggcc
      841 tcaccgtcgc agggttgggc gtggtctcga acggcgacat gacgggggtc aacgtgtcgg
      901 ggctcgggac cgtggccaac cgctcgatgc gcggcctgaa cctggcgggc ctgggcgtcg
      961 tcgccgacgg cgacatggcg gggatcaacg tctcgggcct gggcacggtc gccaacggcg
     1021 tgctgggtgg ggtgagcgtg ggcggcctcg ccgtcgtggg gaaccgcggg gtgtggggga
     1081 ttgccaccgg cggtctcgcg gtcgtgtccg acgaggccgt ggacggcgtg agcgccgcgg
     1141 gcctggccgt ggtggggaac gggcgggtgc ggggaatcgc ggcagcggga ctcgccgtcg
     1201 tcacggacca gaccatgacg gggctggccg tcgccgggct ggcggtggtc ggcggccagg
     1261 gcgtgcgcgg agtggcgatc gggggagtgc acgtcgaagg gaggtggatc gaggggttgg
     1321 ccatctcgcc catccggacg cgcacctggg acctgaccgg tgtgtcgatc gcgggctaca
     1381 accgcatcaa gggcgagcaa cacggtctca ccatcggtct ctacaactac gcgaagcagc
     1441 tgcacggtgt gcagctcggc ctgatcaaca acgcgcgcaa caaccacggc ttgcttcgcg
     1501 tcctgcccct ggtgaatctc cacctccgtt gacctagcgg gtctccggtt cgtgcctcac
     1561 ctttcagccc tcagtctgcc cttgagggcc gtcatgccgc gacttgggtt gctcgccgtc
     1621 ctgctctgtg ctccctgctc tctgctcgct gcccaggcat ccccatacac tccgccgcac
     1681 ctgcgggctc cacagggctt ccgcgtcttc atcgttcccg acatggaagg gatgggaagc
     1741 gcggtggaca tccacgaggt gatcgccggc aacgaaggag agcagtaccg gacgctaacg
     1801 agtcccgact actgggatca tttccgcggt ctgctgacac gtgaggtgaa cgcggccatc
     1861 gccggggcgc gcctcgcggg cgcccgctcc ttcgtcgtga acgaggggca cggcggcaac
     1921 ctgttcgcca atatcctccc ctgggacctc gacaccggtg ccattctcat ccgcgggttt
     1981 cccaagccgc tggtcatggt gacggggatc gactcgaccg tagggacgct gatgttcacc
     2041 ggcgcccatg cggggcccgg gcaccccggc atcctggcgc acaacttcgc gttcgacacc
     2101 ttcacggtga acggcaagag cctgaacgag gtcgggatca acgcgctgat cgcgagcgag
     2161 tacggcgtcg cggtctccct cgtttcgggc gacgacgctg agatcgcgga gacgcgggcg
     2221 attctcggga gcggcttcat cgggatcgtg acgaagtacg cggtgggccg catggcggcg
     2281 atcacgttca gtcccactca ggtccggcgc tggctgcgcg actcggccgc ggtcgcggtg
     2341 cgccgcgcca tgcgcgggga cttcaagccg ttccggctcg acaagcccta tcacgtcgcg
     2401 ttcgccctgc ggcgcagcta tccggacgtc gtggtggccc gtgtggactc gctcaactgg
     2461 accgggctcg agaagaccgg cgagcgctcg taccgcctga cgaccgacga cgcgcggcag
     2521 atggggtatc tgctcgacgc gatcgaacgg gtggtgctgc ggtgagcgcg cgcccactcc
     2581 gaatgctggc gctggcggcc ctgccggcgc tgctcgccgc ccagcagcct ccccaggccc
     2641 agccgccgca gcaacccccg cgcgacacca caccccccga ggcgtactac ggcgtcaccg
     2701 agtaccagca ggcccgctcg aagctcgcag cgctgatgca gcctactggc ttcacggtgt
     2761 acatcatcgc cgacatggag gggctcgccg gcgtggtgcg caacgccacc gagatgcgtc
     2821 ccgaggcgcg cggcgggcat gatccgacgc acgaggcttt tcgcaaggag ctggcggacg
     2881 aggtcaacgc ggcgatcgcc ggggcgcggc tcgccggtgc cacgcagttc gtcgtgaacg
     2941 aaggccatgg cgggacgctg ttccgcaaca tccatcccga ggacctcgat cccgcggcga
     3001 tcctggtgcg cggctatccc aagccgaacg tgatggagac cgggctgaat ccgaacgtgg
     3061 atgcgctgtt catcatcggc gcccatgcga acgcgggcac gccggggatc atcgcccaca
     3121 gttacgcgtt cgactcgttc accgtgaacg ggcatgtact gaacgaagcc ggcatcgccg
     3181 cgttcatcgg cggcgagatg ggcgtgcccc tggccctcgc cgcgggcgac aacgtgctca
     3241 ccgccgaaac gcgcgggatg gtgggcgagc tcgagacggt gacgacgaag atcgccgaga
     3301 gccgcagcgc ggcggcggtg ttcccgcccg cgatggtgca acgccagatc cgcgccgccg
     3361 cggcccgcgc ggtgcgcggc gtgcggaccg gccgcatcaa gccgctgcac atcgagcgcc
     3421 cgtataccgt tgccttctgc acgcgtccgt cgttccagcc gtgggtcccc gaggccacgg
     3481 cgcggctcgc cggggtggag cggatcgcgg gcgattccac cggccgctgc ttcacgtatc
     3541 acacggactc ggcggaggcc gtcggcacct tgctcaacga gatcgagtgg attgtgctga
     3601 aaccgtgaag cgtcgtcggt catcggtcgt cggctatcgg ttgaacgaca ggagggatca
     3661 atgagccgct cgttgcgatt catgctcggc attgtcgccg gactcgctgc cgcgccgttg
     3721 gcggctcagg agaccgcatc cgacacgctg ctcaccatca atcactacct cgattgggag
     3781 caggtgagcg acccgcagct gtcacccgac gggtcgcaga tcgtctacac gcggacgtgg
     3841 gtgaatcggc tggaggatcg ctgggagtcg gccttgtgga tcatgaatgc cgacggtacg
     3901 aaggcccggt tcctgaccaa gggttcgagc gcgcgctggt cgcccgacgg cagccgcatc
     3961 gcgtacctcg ccgacgggga gcccaagggg acgcaggtgt tcgtccgctg gatggacgcc
     4021 gagggcgcca cctcgcagat cacgcgggtt accgaatccc ccggtgagct gcgctgggcg
     4081 ccggacggga aatcgctctc gttcgcgatg ctggtgcggc acacgacccc ctggaccatc
     4141 agcatgccgg ccgcgccgac cggggccagc tggaccgcgg cgccgcgtgt ggtagaccgg
     4201 atgcactatc gccaggaccg ccgcggcttc atggaggacg gctacctgca tctgtttctc
     4261 gtccccgccg acggtggaac cccacgccag ctcaccagcg gcgactggaa cgtcggggcg
     4321 cgctttgacg gcctttcctc gggcgtgggc tacgactggt cgcccgacgg caagtccatc
     4381 gtggtggact ggttcaagga tgcgggcgcg gaccgcaact accgggggtc gcgcctctac
     4441 gtcgtggacg tcgcgagcgg cgcgatccgc cagctcacga cgcgtcccgg cacctggaac
     4501 gacccggtcg tctcgccgga cgggcgttct gtggccttca ccggctacga ctccgccgac
     4561 gtcagttacc gggcggaaga tctgtgggtg atcggactcg acggcggcgg gatgcggaag
     4621 gtctcgggcg acctggaccg ggatcccggc ggcctgcgct gggcgcccga cggcaacggc
     4681 ctgtatttcg ccgccggcga tcgcggtacc cagaacgtct ggtttgccgc gctggcgggc
     4741 ggcggccccc gccaggtcac cagcgggacg cacatgctgt cgctcacctc gctggaccgc
     4801 aacctgaacg ccgtcggcac acagagcgat ccctccaatc cggccgacgt cgtgcgctac
     4861 agcctgcgcc gtcccggtcc ggccaccgtc ctcaccgggg tcaacgccga cgtgctgggg
     4921 cgcatccacc tggccaaggt cgaagaggtg tggtacggct cgagcggcgg agcgcggatt
     4981 caggggtgga tcgtgaagcc gccgtcgttc gacccgtccc ggaagtaccc gctcatcctc
     5041 gaaattcacg gcgggccgca cgggatgtac ggcgtcgggt tcaactacat gttccagaac
     5101 ttcgccgccg acggctacgt ggtgctgtac gtcaacccgc gcggcagcac cgggtacggc
     5161 tcggcgttcg gcaacgcgat cttccacgcc tacccgagcg tggactacga cgatctcatg
     5221 gccggcgtgg atacggtgat cgggcgcggc ttcgtggaca ccacgcgcat gtacgtgtcg
     5281 ggctgctccg gcggtggcgt gctctcgtcc tgggtgatcg ggcacacgca ccggttcgcc
     5341 gcggcggcgg tccgctgccc ggtgatcgac tggatcagca tggcggggca gaccgacatc
     5401 ccgctgttca cctacaactt cttcgacgcg cccttctggg aaaagccgga ccagtggctg
     5461 aagcagtcat cgctgatgta tgtgggcaac gtcaccacgc cgacgttgct gatgacggga
     5521 gagctcgatc tgcgcacgcc gatgccgcaa accgaggagt tctactcggc cctgaagtac
     5581 cggggcgtgg aggccgtgat gctgcgcttc aacggggagt accacggcac cggctccaag
     5641 ccgaccaact tcatgcgcac ccagctctac atgatgagct ggttccagaa gcacggcggc
     5701 cagcaggcag cagcggcctc ctcgtcgcag tgacggcatc cttaccggaa gcgtggttgc
     5761 gtgggccggt cgagggcatc gcgccggtgc tccagccctc ggcgcacgcg ttgatccagg
     5821 cgtcggagga gctgggggcg gccgtgaccg gcctgccggt ggcggcggtc tgggctgccc
     5881 ccggtggcgc cgcgtcgatc gggttccacc tgcggcacat cgccgccagc actgaccgcc
     5941 tcctcacgta cgcacgcggg gaaccgctga cggcggagca gctgcgagcg ctggccgagg
     6001 agaagatgcc gggcacgccg cccgtcgaag gtccggcgct cctggcgcgc gcgcaggagc
     6061 gcgttcgcgc ggcactcgac gcctatcgcg gcgtcgacga gggcatcctg ttcgagccgc
     6121 gtgccgtcgg tcggaagcgc ctgccgacca cggtcttcgg cctgctcgcc cacatcggcg
     6181 agcacgcgat gcgtcacacg gggcaggtcg tcaccacggc caggattgtc cgggccctga
     6241 gcccggatta ggacgccggg gcgagcagga tccgggcgtc caccgccgtg accccgtggg
     6301 gaaacgccag cagcgggttg atgtccagct cgacgatttc cacgaagtcg gtcgcgagct
     6361 gggccacccg ctgcagcgct tcccccagcg ccgcaaagtc gaccggcggg gcgccgcgca
     6421 cgccgtcgag gatcgccacg ctcttgatcc ccttcagcat gtcgtgcgcc tcgagcggcc
     6481 cgatgggagg gatgcggaag acgacgtcgc gcagcgcctc gacgtagatc ccgcccagcc
     6541 cgaacatcac gagtgggccg aacgcacggt cacggctcac gccgacgatg gtctctcgcc
     6601 cgcccggaac catgcgttgc accaggatcc cgtcgatcct ggcctgcggc gccgcgcggg
     6661 cgacgccgtt cagcatctcc gcacacgcct cctgcacctc cacggccgtc gtgaggttga
     6721 tgcggacgcc gccgacttcg gtcttgtgga cgatctgcgg cgagacgacc ttgagcacga
     6781 cgggatagcc gatctcggtg gcggcgtgcg cggcgtcctg gggatcgcgc gccagccgcg
     6841 cgggagccgt cggtatgccg tatgccgtga gcacgcccag tacctcgagc tccgagagtt
     6901 tctggcggcc ggcgctgcgg gcctgggcga gcagccgggc ggcgccgtcg tggtccaccg
     6961 ggagcggttt gtacgccgtc tcggggcggg cgcgccatcc ccgcacccgc cgcagggcgc
     7021 ccaacgcccg ggccgcggat tcggggaaga tgtaggtcgg aatgccggcg cggtgcagct
     7081 cggcttttcc ctcctggagc cctgctcggc ccatgagcac cgccaggacg ggcttggacg
     7141 gatgtccgcg cgcggcggcg gcgatcgatt cggcgacgtc ctccgaccgg atgccgagcg
     7201 gcggcacgaa gatggcgacg gccgagtcca cgtggccgtc ggccagcacg gcatcgagcg
     7261 ccgcccggta gtcggccgcg gtcgccgagg cgatcatgtc gaccggattg cgcaggctcg
     7321 cctcgggcgg gaacagcggc cgcagccgtt cgatcgtctc cggcgtgagg tccacgagct
     7381 ccagtccctg cggctcgagc gcgtcggcga tgagaatccc cggaccgccg gagttggtga
     7441 gcaccgccgt gcgccgggag cggggcaggg gcgggccgtt gaacgccatc gccatgtcga
     7501 acagctcttc catggaagtg gcccgcagca ccccggcctg ggtgagcaac gcgtccaccg
     7561 cggcatcgct cgcggccagc gcgccggtat gcgaggcggc ggcccgcgcg ccgaccttgg
     7621 agcggcccga cttcagggcg atgatcggct tctgcttcgc gatgcgggag gcgatctcca
     7681 gaaaccgccg cgggttgccg aagttctcca cgtacatgag gatcactttg accgcgtcgt
     7741 cgtgctccca atgcagcagg agatcgttgc cgctcacgtc gggcttgttg ccgaccgaga
     7801 cgaattgtga aatcccgatc ccgtactccc tggcgtagtc gagaacgctc atgcccaggg
     7861 ccccggactg cgacacgaac gccgccgccc cgaacggcgg catcgagggg gcgaaggtcg
     7921 cattgagcga caccgccggg tccgcgttca gcacgcccat gcagttcggc ccgaccatcc
     7981 gcatgcggtg gcggtgcacg atttccacga gctgccgctc ggccgcggca ccggcttccc
     8041 ccacctcctt gaagccggcc gagatcacca cgagaccgcg cacgcgcttg gtgccgcact
     8101 gctcggcgac ggtcggcacg tgctcccgcg gcacgacgat caccgccatg tcgacggggt
     8161 cggggatctc caggaccgac gggtaggccc tgatggagtg gacgttggag gccttggggt
     8221 tgaccgggaa caccgcgccg gtgaagccgt accgcacgat gttcgcgagg atctcattgc
     8281 cgatggtatc gggacgccgc gacgcgccga tgacggccac ggagcgtggt cgcaggatgg
     8341 gctcgagatc aggcatgacg gtcaaagatg caggcggcgt atccgagcac ttttgcacct
     8401 cctttgagca ggctgggggt ggggcgccag ccccgtccca gccgcgcgtc gagcagctct
     8461 cgatcggtgg ggcgatgatc ccagacggcg accgccgtca gggcgcccag tcaggtgacg
     8521 tcgaacgcga gcgtgagccc atcgcgccac ggcaccagca catcgtgtgt gtactcgtcc
     8581 tgggtgacga ccgccggtgc ggtcaccggc ggatcgaggg tgcgtgccca ggccagcacg
     8641 tcgcccaggt tgcgcaacgg cgcgagcgcg ctccggaggg cggcgagcag cggcggcgcg
     8701 gctcccgcgc gatcgaacac ctccgtcatc cggtgccgcc caggcgttcg cgcagcatcc
     8761 ggaacggcgc ggcgagccac ccctcttccc gggcccgctc ctcccgcgcg gcgtgcgtcc
     8821 atgccgccat cactgcgccc cgatcgagga atccgaccac gcgccccggt tcaccgcggc
     8881 tcaccaccgg cagctgtccg atgtcgtggt ccaccattct ggccgtcgcg acctcgagca
     8941 gctcgtcggg atgcgccacg acgatgtcct tcgttcccga ttgcaggacg gtccgcccgg
     9001 cggtgtcggg ctgccacacc gtgcgcgtca ggtcgccccg ggtgatcagg ccgaccaggc
     9061 ggccgccctc gtcgacgatc ggccacgcct gacgccggcc gacgacgggg tcgaggtgag
     9121 cgagctgcag gaacagggtg tccagcggca gattggcggg aatggcggga acgtcgcgtt
     9181 ccatcacgtc ctccacccac agccgatgca gggggctgac gctgtactcg cgcatcacgt
     9241 gatggccgcg ccgcgccacc ttctcggtga ggatggagcg cttcatgagc agcacggtga
     9301 cggcgtcggc ggcgacacag gcgatcagga gaccgggcag gacgttgatg tcatgcgtca
     9361 gctccagcat gaacgcgatc gccgtaaagg gcgaccgcat cgtcccaccc atcatcgctc
     9421 ccatcccgat accggcccag agcccggcgt ccgcgccggg cagcaatgga gacagcaatg
     9481 cccccatcgc cccacccatg atcagcaacg gggcgagcac tcctcccgaa gtgccggacc
     9541 cgagagccac cgcccaaatg accgccttgg tcacgagcag gccgagcagc aatgccccca
     9601 cctggtgtcc ggcgagcagg tcgtggatgg tgtcgtaccc gaccccgagc gcccgcggat
     9661 agagccagcc gcccaccccg atgaccaggc cgccgagcat gggccaccac atccaatgga
     9721 tgggcagttt gcggaacagg tcttctgctc cgtagacgag cgcggtgagc aatcccgatc
     9781 cgaggccggc cgcgatgccc atgcccgccg cgcatgccag caccacccag gagagcgtgg
     9841 cgtgcggtgt gacggggaag atgggccccg acccgaggag cgggacgcgg agcagtgcag
     9901 cgaccgctgc cgccgcggcg accgggatga agctctgggg cttccattcg aacagcagca
     9961 gctccaccgc cagcagtacg gccgcgagcg gcgtcgcgaa caccgccgcc atcccggctg
    10021 cggcgccggc gacgagcagc gtcttgcgct ccgcgggaga cagatggaac gcctgcgcga
    10081 acagggagcc gaacgcgccc ccggtcatga tgatcggccc ctcggcgccg aagggaccac
    10141 ccgtgccgat cgcgatcgcc gaggagagcg gcttcaacac cgcaaccttc acgtccatgc
    10201 ggctgccgcc gatcaggatc gcctcgagcg cctcggggat gccgtgcccg cgaatgcgtt
    10261 ccgatccgta gcgcgccatc aaccccacga tcaggccacc gagaaccggg atcgaaacgg
    10321 cccacagccc gggatgggcg tcggcggggg aggcaagggt actggcgacc tcgtgatagt
    10381 agaagacgtg ggtgaagaag ccgatcaggc ggagcaggcc gagcgccact aacgcgccca
    10441 cgcccccgat caggcacgcc atgagcgtca gcttcaacac ccgagtggtg gtcgtgaagt
    10501 cggccaggct ctctgctcgt tccggggtgg ccgatgcggt gtgggtctgg ggctgttgcg
    10561 atcggttcac ggtttgggaa gctaatgcgt catagcagct tacgcgaccg tccttccgaa
    10621 atacgaacgt tcgtgctatt gtaggcgatg ggcaagggcg acctcactcg acaggccatt
    10681 ctcggacgcg ccaccgggct cgcgagcaca gccggcctcg agggcgtgac gatcggcgcg
    10741 ctcgccgccg agctgcaact gtccaagagt ggtctctttg cgcacttcca ctcgaaagag
    10801 gcgctccaac tccaggtgct cgagttcgcc gcggcgcaat tcgtcgagca cgtgattcgc
    10861 ccggctctcg ccgagccccg cggcgagcca aggctgcggg cgctgttcga gcgctggctc
    10921 gactggtcgc actccagccc gctgccgggc gggtgtctgt tcgtccaggc gtcggtcgag
    10981 ctcgacgatc gtccgggttc ggtgcgcgag cggcttgtcg ggatccagcg gcaatggctc
    11041 ggcgttctcg caacgtcgtt gcagaaggga atcgaggccg gggcgtttca tgacggcgtc
    11101 gacccggaac aattcgccca ggacgtgtac ggggtgatgt tggcgtatca ccatgcgtcg
    11161 cgcctgctgc atgacccgcg ggccgaaacg cgcgcccggg gtgccttcga aacgttgctc
    11221 tcggccgcgg gcgtcgctca ctgaaacaga ggcagattac cgatgcgact gctccgactt
    11281 ggctttcgtt cgctgcaggc ggtctccccg tcccgggctg ccgctctggc cgagcggttg
    11341 ttcttcaccc caccccgctc acggctgacg cccgacatgc gcgccgagct ggagcggggc
    11401 aggccgttca ccgtccaggt ggagggccac cgcgtcgcct gctggtcctg gggctcgacg
    11461 ggcctaccca ttgcgtatct ggtgcacggc tggggatcgc gtggcgggag gctcacggcc
    11521 tacgtgcggc cgctccaggc cgccggtttc cgggtgatcg cgttcgacgg catcgggcac
    11581 ggcgcctcgg aggggcagct gagctcggca ccgcagctgg cgcgcacgct cagcgcgctc
    11641 gccgacgccg tgggtccggc gcactcggtc gtcgcgcact cgctcggcgc cgtggtgacg
    11701 accctcgcca tggaatgggg attgccggtc tcgcgagccg ccttcctcgc gcccaacgcg
    11761 gaccccatcg cgcatacgct gcgctgggca catcggctcg gcatgcggcc cgacgtggtg
    11821 gcacagatgc gagccgccac cgagcgccgt atcgccttct cgtgggatga tctcgacgtc
    11881 gtgacgatgg cccgccgccg cacgaccccg ctgctcgtga tccacgacgc taccgatgag
    11941 gtggtgccgt ggatggaagg cgccgccatc gtcggcgcct ggcgcggcag ccggttcgtc
    12001 acgactcgcg gactgggaca ctcgcccctc gtccggaacc ccgaggtcgt gacacaggtg
    12061 gtggagtttc tcggaactcc ggagggctcc cgcgcgcgac cggcacgggt gtccggcatc
    12121 gagcacgaac tgttctaccg cgaatggcgg ccttccctgc cgccgctgtc agcctaggag
    12181 ctgcggggcg tcgctcagga ccgcctcagc cacgcggtcc gccagcgcca tgatggtcac
    12241 gtaggggttg atctcgaggg cgtcggggaa gagcgaggcg tcggcgacga acagccaggg
    12301 gatgccgtgc acgcggcccc aggcgtcggt caccgagtcg gcggcggacg tccccatccg
    12361 gcatccgccc atcaggtgag cggagctgat cgcctgtttc cccggcttcc agtgtcgcgc
    12421 atcgatcagc gtgtcgagcc ggcccgcatc gtgggcctcg atggtcggag gctctgcgaa
    12481 cggcgcgtgc acccgcaccg cgccggcggc gaagaaaatg cgagccgcgg cgcgattgcc
    12541 ccgcaccaac cccgtcaccg tctcgcgtga atacgcgtag tgcacgaccg ggttgccgtc
    12601 ccggtcgatc gcgacgcgat tgccgtcggc ggcccggtcg atgcccagca cgaggatctg
    12661 ctgctgtcgc ggaaaggccc gcattagagc gctgtgctcg gggccgaacc cgttcagcgc
    12721 cttggcggtg gtgaagggga agtacatgca cgtctcgagc acgaaccgct cctccgccgc
    12781 acgatccacg aagaagctct tggggtgacc gacgtcgttc gtaatcggca ccgcatgctc
    12841 cgccaccagg atcaacgccg ggtggcaggt gaagccctcc ccgagggagc ggggggtggg
    12901 gagcggggag cggagtagca gggggggaga tccgagcgct ccagcggcgc acaccacgat
    12961 cttcgcttct acccggtact cacccgccgg ccactccgac ggccggccct tcgcaccggc
    13021 aaggagctcg ctcacccgca ccgtgagcga gcggtccggc gtgatccgca ggacttcggc
    13081 gcgcgtaacg acttccaccc cggcgcgttc ggcggcgggg agctgcacgc gatgcgtccc
    13141 ctgcttggcc tggttggggc atcccaggtt acacagggac gagccccggc agcccttcac
    13201 gttcacgggg aactgctcga cggcatatcc ggccttccgg gctcccgctt cgaacagacg
    13261 attgttgtcg ttgagctcct cgagcggaag gcgatggacg ttgttctcgc cgagatagcg
    13321 ctgggagcgg cgcaacacgt ccgcatggct cagacccggc acggcccacc gctcgatgac
    13381 ccgtgccggc gcgatcagcg aagtgccggt gtagacgatg gtcgatccgc cgtagccgtg
    13441 cgcgaaggcg agcgtcatcg tgccgtcggc actgaggaag ccgccgccgt cgtggtacag
    13501 cgcgctggcc atctcgagct cgcggcccgt gaattcgtcg tccccgaagc gcggcccctg
    13561 ctcgagcacc agcacccgcc ggcctgcctg ggccagcggc gccaggagct gagcgaccgt
    13621 accgccgccc gctccggagc cgacgatcac cacatcggag gatcgcgagg ccggggtcac
    13681 gacaccctcc ggcgcgcggt ccagccgccg gggtgggcgc ggtacccgat ctcgtcccgg
    13741 gcctccggcc gcccgtagta gcccatcagc agcagcgtgc gcagccccca cgtcccacgc
    13801 cgtaccagga gcagcggggc atcctgcagg ctcttcagaa agcgcgtccg gcgcgccgca
    13861 tcgaggcgcg tgaaccggcg cccgaagcgc accacgggaa gccactccat gagacggagg
    13921 aacaggagga actgccgttg cagcgcggcg ggccgggcgg cgagtgcggc ggcgacggtg
    13981 cgctcgagcg ccgcccatcc cgcctcatcg aggcgcgtcg cctccggcac cacggtcacg
    14041 ccgaccgccc gcagcaccgg tcggacggag gtcagcacag ggaaaaccta ccatactgct
    14101 tatcttttgc caggaacccc gacgggaggg gacgttgtcg gagcgagctg atgaattcgg
    14161 cggtctggaa gcggcctgcg ggcaattcga gtccaccaag caggaggccg cggagctcgt
    14221 cgcgcccctg accgatgccc aggtcaactg gtgcccggca ccaggccgat ggtccatcgg
    14281 ggaatgtctg ttgcacctgg cgaccagtac cgatgccgcg cttcccgcgc tcgacctggc
    14341 catcgaacag gctcagattc gcgggtggac ggcctgcggg ccgtaccggt acggctggtt
    14401 cacgcgctgg aacgttcgtt cgatggagcc acccccggcg ttccgcgtgc gcacgtttcg
    14461 catcttcgag ccgcccacga ccgccatgcg gggaggagcg gtggcgcgtg cgctcgccga
    14521 atcgcgcgat cgactgctcg agcggacgcg ggtggcggcc ggcgtggacc tcaagcgagc
    14581 ggtcgtcgtg tcgccggcat tgcgactgtt ccgcttgccc ctgggggggt atctggcctt
    14641 cctggcggcc cacgaccgcc ggcatctgtg gcaggcccga caggtgctcc gggcgtccgg
    14701 gttcggtcag gcgtgaggct ccgacgctgg catgggacgc gattcctccg caccatcaga
    14761 gtgcaacctt cacggtgtgg caggcgtcta gaagcaagac cgccacggtt cgaggctctc
    14821 caatgaccca acgctccatg cttcccgcag tcgtagtggc cggcacgctc ctgggctgcg
    14881 gtgcctcgca gtctccagtg ccggtggtcg gccgtgaagt cgacgtctcc cagctcgccg
    14941 gcgagtggtc cggtgattat tcgagcgtgg aatccggtcg cagcgggagc atcgtgttca
    15001 agctcgtcgc cggagccgac agcgcgatgg gtgaggtcat catgacgccg cagtggctcg
    15061 cgcgtccggg gggcgccaat acgcaacccg gacccacgac gcaacccgtc tcgcagccgc
    15121 tcaccatctc cttcgtgcgg gtctcgggag ggcaggtgag cggagccctg cagccgtacc
    15181 ccgacccgaa ctgcggctgc acgctccata cgacgttcgt gggccggctg cagggcgaca
    15241 cgttgcaggg gagctactcc tccgtgcacg aacagacgcg cgacgtccag cagggacgct
    15301 ggcgcgtcgt gcgcaagcag ccctgagtgc gcgccgcccg ctcggctggt tgcccgcgat
    15361 cgcgggcctc gccgtcgcgc gcctgagcgc acagggcccg gcgccagcgg gtccgcgccc
    15421 ctatgacgag cctccccaca agcaggcgac ggccgagcgc gtgccgccgg gagccgtccg
    15481 gatggacggc cggctcgacg attcggtctg gctcgcggcc cgttggatcg acgacttcgt
    15541 cgagaagatg ccggtcgagg gcgggacgcc gaccgacagc atgcgtctcg ccatcgtgta
    15601 cgacgatcac gccctgtacg tcggggcccg gatgtactcc cacgacccgg cccacattca
    15661 ggcgccgctc agccgccgcg acaatacgtc gcaggccgag cacctctggg tgtccttcga
    15721 cacgtatcac gaccggcgca ccgcctactc gttcggcgtg accgcgaccg gggtgcggat
    15781 ggactggtac cacccccggg atcacgaata cgacatcgat cccacgttcg atccggtgtg
    15841 ggaggcgaag gcggtcatcg acagcctggg ctggaccgcc gagatgcgga ttcccttttc
    15901 ccagctccgc ttcaaccgcg gcgacgctca ggtctggggg ttcaatgccg accactggat
    15961 tccgtcgcgc aacgaggatg tcttctggat tcccgtgccg cagaaccgga ccggctggag
    16021 ctcgtggatg ggcgatctgc aggggatccg ggacatcgcg ccgtcgcgcc ggctcgaact
    16081 gctgccctac acggcgggca gcgccacgct caccggcacg cgcgatgcgg ccgacccgtt
    16141 cgacgacggc cgcaatctca aagggcggtt cggcaccgac ctgaagatgg gattggggcc
    16201 caacctgacg ctcgatgcga cggtcaatcc cgatttcggc caggtggagg cggacccggc
    16261 ggtggtcaac ctcacggcat tcgaggtgtt cttcgacgaa aagcggccgt tcttcctgga
    16321 agggagccac ctgctgcgcg gcgacgggcc gagctacttc tattcgcggc gcatcggcgc
    16381 accgccgcgc gggagcgcgg cgggtgattt cgtagactat ccgtccaact ccaccatcat
    16441 cgccgcgggc aaggtgacgg ggcagacggc ggctggcttc tcggtcggga cgctcgcggc
    16501 ggtcaacgcc cgagagttcg cccgcaccta cgatacggcg acggcgacgt tcacccggct
    16561 ccaggtggcg ccgcccacgg cctacggagt ggtgcgagca cagcagcagt tcggcgccac
    16621 tgcctcgacc atcggcttca ccctcacggc cgtgcgacgc gacttcgcgc cgggatcggc
    16681 gctcgactcg ctgctcgacc gccaggcgtt cacgggcggc gtggacgggg tgattcgctg
    16741 ggcgggcggg gtctatcggc tgagtgggta tgcgggattc agcgcggtgc ggggggatgc
    16801 ccccgccctg atcgggatcc aatcgagtcc ggcacatttc taccagcgtc ccgacgccgg
    16861 ttacatccgg ctcgattcca cgcggaccgc gctgaacggc tatacgctcg gtctcgattt
    16921 cggcaagacc ggcggtggcc attggcgcta tgacgcgggg ttcggcgtcg aatcaccggg
    16981 gttcgagctc aacgatgcgg gccggctgtt caacgccgac gggcggacgg gcttggcgag
    17041 cctgaactat cgggagaccc atccgggccg gtggtaccag agcttcggcc tgggcatttc
    17101 cggcaacgcc gagtgggact tcggcgggat ccggcagttc gcggtgaccc agcagaacgc
    17161 ggacctcacc ttcaagaact actggtcggt caacgcgtac ctgtaccaat cctggccctc
    17221 gctcgaccac gcgctgaccc gcggcgggcc gttgatggcc acgccctact cgtgggggag
    17281 cggcgtacgc atcggcaacg gcttcggcgc gaagacctca tggaacgtcc gggtgagtta
    17341 cggcacggac gcgctgggcg gggaggcatc gcagctgagt ggcaggctct cgatccggcc
    17401 cgccaaccgc ctcgagctgt cgatctcgcc gaggtatgcg cgcgagatct cctcccggca
    17461 gtacatcgcg acgctcgatt ccggcgggcc cgccacctac gggcagcgct acctcttcgg
    17521 cgccatcgat cggagcacgc tgtcggcgca gctgcgcttg acctacacgc tgacgcccga
    17581 tctcacgctc gagctgtacg ccgagccctt cgccgccagc ggccggtact acggtatcgg
    17641 cgagttggcg cggggccgga ccacggacct cacgctttac tcgggcgaca cgacggtcgt
    17701 gccgagtctg atccgcaatc ccgccgggga gtacacgctg aaggatgccg gcggataccg
    17761 ccgggacctc ggcaaccccg acttcaacgt tctctcgttc cgcagcaacc tggtgctgcg
    17821 ctgggaatgg cggcccggca gcacgctgtt tctcgtgtgg caacagagcc gcttcgcgct
    17881 ggacacccgg ggggcgctgg tggggccggg agcgctgtgg gacagcttcc gggcgagcgg
    17941 cgacaacttc ctggcgctga aagtgagcta ctggctggcg ccctgagtca caccctcgcc
    18001 ggctccagtg caggctccac cgccacctga atgtcgatcc cgtcgcgcac gctctgggtg
    18061 acgacgcaga aatcctcgaa caattccagg cagcgggcgg cgcgctccgg cgcgtgcggc
    18121 cccagcacgg gcgcgagccg cacgcgcaac tccccgatcc gcactc
//
LOCUS       DBNGZW010000527         8259 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1059253, whole genome shotgun sequence.
ACCESSION   DBNGZW010000527 DBNGZW010000000
VERSION     DBNGZW010000527.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8259)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8259)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8259
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1059253"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2398)
                     /locus_tag="AC16JC_12550"
     CDS             complement(<1..2398)
                     /locus_tag="AC16JC_12550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5165571.1"
                     /translation="MIGLLWRRLRDRMRRERLSAEFSEELRFHQALLARDHRVAGMTA
                     ADADAAARRRMGNRTALAEDVRALWSLRWLDDLGQDIHYALRALRRTPLFTIVAGLTL
                     ALGVGANTAVFSVVNGVLLRTLPFREPDRLLLLSYQERDASPWAFPGLLDRQYEVVRH
                     GTRAFESIATFSSGQATLTGAGDATRLTVANVSPEFFTVLGVGPALGRPFDKAEGAGG
                     GAHVVLLSDGLWRGRFAGDSAIRGRAITLDGLAYVVVGVMPASFAFPSGADLWVPADM
                     RINPHLTMMRPVVGRLRAGVSRDQALAELEALSASFELFPDQPRDRMVARVLSLNALL
                     VGNIERSLLLLAGAVAFVLLIACANVANLLLLRGATRAQEIAVRSALGAGRRRLVRQL
                     LTESVVLFLSGAAAGLPVALAGTRALVALAPAGTLPRLAAIRMDGRVLLFTLGVALAT
                     GLIFGLAPAVLATRRPAVPTPGHGVRATAGPGRLRGALVIAEIALALVLLTGAGLMIR
                     SFQRLQAIDLGFQPAHIMSVTVDLPETKYPGAAAMRAFHDRTVESLAHLPHTAGAAAV
                     NWRPLGDAQIDGTFTVDGRSPPDFNVSKPAVTPDYFRVMGIPLLRGRPFTARDDARAP
                     GVVIVSRSVAERIWPGADPVGQRISMEDHPTPSDWLTVVGVVENVRQERLTQGPVPAI
                     YQPLNQVSRTFFLSHMTFLARTDAAPGEYARAVRATLRDIDPDLPPQALASLQDFIAR
                     IQATPLFQTRMLVVFSLLALALAAVGIYGLLAYSVMERTHEIGIRLALGAPIGRVMRL
                     V"
     gene            complement(2395..2739)
                     /locus_tag="AC16JC_12555"
     CDS             complement(2395..2739)
                     /locus_tag="AC16JC_12555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR5165572.1"
                     /translation="MPQLTPPNRRDLLPGTLDMLVLKTLTVSPMHGYGIAQHLQRLSH
                     DVLQVEEGSLYPALHRMSQRGWIRAEWRRTPNNQRARYYTITAAGRRQLGEQVSGFAQ
                     LMAAISRVMKTT"
     gene            complement(2789..4021)
                     /locus_tag="AC16JC_12560"
     CDS             complement(2789..4021)
                     /locus_tag="AC16JC_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020484078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5165573.1"
                     /translation="MVDAVYRSESRRVLATLIRLLGDFDLAEEALHDAFAAAVERWPR
                     DGVPDNPRAWLVSAGRFKAIDAVRRRARFDASVAKLAREIETHTAEIATDSPGVEDDR
                     LRLIFTCCHPALPPDAQVALTLREVCGLTTEEIARAFLTTPPTVAQRIVRAKAKIRDA
                     RIPYQVPAPADLPDRLDTVLHVVYLVFNEGYSASSGASLVRHDLSGEAIRLGRVLVEL
                     LPNPEVMGLLALMLLHESRRTARASASGELVLLDEQDRSLWNRDQIAEGAALVERALA
                     SHRFGRYTLQVAIAAVHAEAPAAAQTDWAQIVGLYDVLLHADPSPVVELNRAAAVAMR
                     DGPAAGLALIDAILARGDLHEYHLAHAARADLCRRLGRTAEARAAYERALALVRQEPE
                     RRFLERRLGELRDPPPGA"
     gene            complement(4064..4420)
                     /locus_tag="AC16JC_12565"
     CDS             complement(4064..4420)
                     /locus_tag="AC16JC_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011399319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HIR5165574.1"
                     /translation="MRYLCLIYDDEKKVGAMPKGESDAFMGAYFAFTEDIKKSGHYLA
                     GEALQPVQTATTVRVRNGKLSTTDGPFAETKEQLGGFYMIEARDLNDAIQVASRIPSA
                     RMGSIEVRPIMEFDRP"
     gene            complement(4531..5067)
                     /locus_tag="AC16JC_12570"
     CDS             complement(4531..5067)
                     /locus_tag="AC16JC_12570"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR5165575.1"
                     /translation="MKASVFCGTSLDGFIARSNDALDFLPAGGGEDHGFTEFLASVDA
                     VVMGRRTFEVVLTFDGWPYGTKPVVVLSSRPLDIPAFPGAVIERMSGTPAEIVSRLAA
                     HGMEHIYVDGGVTIQRFLAAGLIQRLVITRVPVLIGSGIPLFGPLSRDIVGRHAATRA
                     FPSGLVQSEYLVDRTPAE"
     gene            complement(5781..7940)
                     /locus_tag="AC16JC_12575"
     CDS             complement(5781..7940)
                     /locus_tag="AC16JC_12575"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014896.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIR5165576.1"
                     /translation="MRSRAACRAARSWSIVLLSAAAWVQVARAQALAYPAPHRDAVVD
                     TLFGTPIADPFRWLEQLDGPATVEWVTLQRRLTDDYLARLPGRERIRGRLAALWNYSR
                     TDVPWREAGRLFFTENNGAQPQPLLYTVKGPGDSARVVLDPQDISPDGSIAVGDYSVS
                     PDGRWLAYSEAPGGSGGDTHVRDLATGREQGDVVRDTRGSVCWTFDGNGFFYWRPRPA
                     RPGEDVTAARLEKQYLYHTLGSPQQRDRMIHEWTDARYLYCMLSDDGRRAFLVEEKGS
                     ASWMNVMDLGDPKAPDLSAPLVPLLASREARYTPMGTVGDTLYVFTDLDAPRGRVIAL
                     DLSAGEGARPRGVVPESPDVIQWATVAGDRLALHYLVDVKSRLELFTLDGRPVGEVAL
                     PGIGALGWPVNGRSSAPEVLFSFRSFLSPETVYYYDLRTGTNTPFRPPRVPFDPRPYG
                     TRQVFYRSRDGTRVPMFITAKKGVRLDGRNPVLLMSYGANGITVGPSYRPDLPLWLEL
                     GGVYAVANIRGGGEYGEQWHRAGNLEHKQNSIDDFLAAAEYLVGRRYTVPAKLAVYGH
                     SSGGLLIGAALTQRPDLFGVALPSAGHHDMLRYHRFTVGAGWTTEYGSPDDSVAFRYL
                     LAYSPLQHVRPNVCYPATLLLTADHDETVVPSHSYKFAAALQGVQRCSRPILLHVTPN
                     ASHMYASQEAAIGEWTDIWAFVTAQFGVHVADRGTRR"
     gene            complement(7949..>8259)
                     /locus_tag="AC16JC_12580"
     CDS             complement(7949..>8259)
                     /locus_tag="AC16JC_12580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1152 domain-containing protein"
                     /protein_id="HIR5165577.1"
                     /translation="RLATEAVFAAMPHHISIVSSSILSALAGYYGDHHATPRTEFSEL
                     WINPFMPIYWCFRLAPVAERVLYREDLRETESYRDVTEVIARFRNACSTIRPRRIIPA
                     "
BASE COUNT         1350 a         2784 c         2933 g         1192 t
ORIGIN      
        1 acaccaggcg catcacccgg ccgatcgggg cgccgagggc caggcggatg ccgatttcgt
       61 gggtccgttc catgacggag taggccagca ggccgtagat gccgaccgcg gccagcgcca
      121 gcgccagcag cgagaagacc acgagcatgc gcgtctggaa cagcggcgtg gcctgaatac
      181 gggcgatgaa gtcctgaagc gaagccaggg cttgcggcgg aaggtcgggg tcgatgtcac
      241 ggagcgtcgc gcggacggct cgcgcgtact cgcccggggc agcgtcggtg cgggcgagga
      301 acgtcatgtg gctcaggaag aacgtccgcg agacctgatt gagcggttgg tagatcgccg
      361 gcacggggcc ctgggtgagc cgttcttgcc gcacgttctc gacgacgccg accaccgtga
      421 gccaatccga gggcgtcggg tgatcctcca tggagatgcg ctggcccacg gggtcggcac
      481 ccggccagat ccgctccgcg accgaccggc tcacgatcac gacaccgggg gcccgggcgt
      541 cgtcgcgcgc ggtgaacggg cgcccgcgca gcagcggaat tcccatcacg cggaaatagt
      601 cgggggtcac cgccggtttg gagacgttga agtccggcgg cgaccggccg tcgaccgtga
      661 acgtgccgtc gatctgtgcg tcgcccagcg gacgccagtt gacggcggcg gcgccggcgg
      721 tgtggggaag gtgcgccaga gattccacgg tccggtcgtg aaatgcccgc atcgccgccg
      781 cgccggggta cttcgtctcc ggcaggtcga ccgtgacgga catgatgtgc gcgggctgga
      841 atccgagatc gatcgcctgc aggcgctgga agctccggat catcagtccc gcgcccgtga
      901 gcagcaccag ggcgagcgcg atttcggcaa tgacgagcgc gccgcgcagg cggcctggtc
      961 cggcggtcgc gcgcacgccg tggcccggcg tcgggaccgc gggtcgtcgc gtcgccagca
     1021 ccgcgggcgc gagtccgaag atgagcccgg tcgccagcgc gacgccgagc gtgaacagca
     1081 gcacccggcc gtccatgcga atggcggcga ggcgcggtaa ggtgccggcc ggcgcgagcg
     1141 cgaccagcgc gcgagtcccg gcgagggcga ccggcagtcc cgctgccgcc cccgacagga
     1201 acagcacgac gctctcggtc aggagttgtc gtacgagtcg ccgccgtccg gcgccgagcg
     1261 cgctgcgcac cgcgatctcc tgcgcccgcg tcgccccgcg cagcagcaac agattcgcga
     1321 cattggcgca cgcgatgagg agcacgaacg cgaccgcgcc ggcgagcagc aggagcgagc
     1381 gctcgatgtt gccgaccagc agggcgttga gggacaggac gcgcgccacc atccggtcgc
     1441 gaggctggtc ggggaacagc tcgaaggagg cggaaagtgc ctcgagctcg gccaatgcct
     1501 ggtcacgcga gacgcccgcg cgcaaacgcc ctaccaccgg ccgcatcatg gtcaagtgcg
     1561 ggttgatgcg catgtcggca ggcacccaca ggtccgcgcc cgacgggaag gcaaaggaag
     1621 cgggcatcac cccgacgacg acgtaggcga ggccgtccag tgtgatcgcc cggccccgga
     1681 tggcgctgtc gcccgcgaaa cggccgcgcc acaggccgtc gctcaacagc accacgtgcg
     1741 cgccgccgcc cgctccttcg gccttgtcga acggccgtcc cagggcgggc ccaaccccca
     1801 ggacggtgaa gaactccggg ctgacgttcg ccaccgtcag ccgggtcgcg tcgccggcgc
     1861 ccgtcaacgt cgcctgcccg ctgctgaacg tcgcgatcga ctcgaatgcc cgtgtcccgt
     1921 gccggaccac ctcgtactgc cggtcgagca ggccggggaa cgcccacggg gaagcatccc
     1981 gttcctgata cgacaacagg agcagccgat cgggctcgcg gaatggcaga gtgcggagca
     2041 gcacgccatt gacgacgctg aacacggcgg tattggcccc gacccccagg gccagcgtca
     2101 gcccggccac gatggtgaac agcggcgtgc gccgcagcgc gcgcagcgcg tagtggatgt
     2161 cctgccccag gtcgtcgagc cagcgcaagc tccacaaggc ccggacatcc tcggcgagtg
     2221 cggtgcggtt tcccatccga cgccgggctg ccgcgtccgc gtcggcggcc gtcatgccgg
     2281 cgacccggtg gtcccgtgcc agcagcgcct ggtggaagcg cagctcctcg gagaactcgg
     2341 cgctcagccg ctcgcgccgc atccggtcgc gcaggcgacg ccacaggagt ccgatcacgt
     2401 cgtcttcatc acgcggctga tcgccgccat cagctgagcg aagccggaca cctgctcgcc
     2461 gagctgccga cggccggcgg cggtgatggt gtagtagcgc gcgcgctgat tgttgggcgt
     2521 gcggcgccac tcggccctga tccagccgcg ctggctcatc cggtgcaacg cgggatagag
     2581 cgagccctcc tccacctgca acacgtcgtg cgacaggcgc tgcaggtgct gggcgatgcc
     2641 gtagccgtgc atcggcgaga cggtcagcgt cttgagcacc agcatgtcga gcgtgccggg
     2701 gaggaggtcg cggcggttcg gcggggtgag ctgtggcata tagatcgtct aagggataga
     2761 ctatctatgc cattggcccg aggcggtgtc aagccccggg gggtgggtcc cgcagctcgc
     2821 cgagtcgccg ttccaggaac cgccgctccg gctcctgccg cacgagggcc agggcccgct
     2881 cgtacgcggc gcgcgcctcg gccgttctcc ccagccgccg gcacaggtcg gcacgggccg
     2941 catgggccag gtggtattcg tggagatcgc cgcgcgcgag gatggcgtcg atcagcgcga
     3001 gcccggccgc cgggccgtcg cgcatggcga cggccgcggc gcggttcagc tccaccacgg
     3061 gagaggggtc ggcgtgcagc agcacgtcgt acagtccgac gatctgcgcc cagtcggtct
     3121 gcgccgcggc aggtgcttcg gcgtggacgg cggcgatcgc cacctggagg gtgtagcgtc
     3181 cgaagcggtg cgaggcgagc gcgcgctcga ccagcgccgc gccctcggct atctggtccc
     3241 ggttccagag cgagcgatcc tgctcgtcca gcagcaccag ctcccccgac gccgacgccc
     3301 gtgcggtccg acgggactcg tgcagcagca tcagcgccag caatcccatc acctcggggt
     3361 tgggaagcag ctccactaac acccggccca gccggatcgc ttcgcccgag aggtcgtgcc
     3421 gcacgaggga agctcccgag gaggcggagt agccctcgtt gaacacgagg tagacgacgt
     3481 gcagcacggt gtccagacga tccgggagat cggccggagc aggtacctga taaggaatgc
     3541 gggcgtcgcg gatcttcgcc ttcgcccgta cgatccgctg ggccacggtc ggcggcgtcg
     3601 tgagaaaggc gcgcgcgatc tcctcggtgg tgagcccgca cacttctcgc agggtgagcg
     3661 ccacctgggc gtcggggggc agggccgggt ggcagcaggt gaagatcagc cgcaaccggt
     3721 cgtcctcgac gcccgggctg tccgttgcga tctccgccgt gtgcgtctcg atctcccggg
     3781 ccagcttcgc gaccgacgca tcgaaccggg cacgccggcg cacggcgtcg atggccttga
     3841 agcggcccgc cgacacgagc caggcccgcg ggttgtccgg gaccccgtcg cggggccagc
     3901 gctccaccgc cgccgcgaac gcatcgtgca acgcctcctc ggccaggtcg aagtccccca
     3961 ggaggcggat cagggtggcg aggacgcggc gcgactcgga gcggtagacg gcgtccacca
     4021 tctcgcgcac cgggtcggcc gcttccgcgc tcacctcacg tccctacggc cggtcgaact
     4081 ccatgatcgg gcgcacctcg atgctcccca tccgcgcgga cggaatcctg gaggccacct
     4141 ggatggcgtc gttcaggtcg cgcgcctcga tcatgtagaa tccgcccagc tgctctttcg
     4201 tctcggcgaa cggtccgtcg gtggtggaca gcttgccatt gcggacccgg acggtcgtgg
     4261 cggtctgtac gggctgcagc gcttctccgg ccaggtagtg gccgctcttc ttgatgtctt
     4321 ccgtgaaggc gaagtaggcc cccatgaacg cgtcggactc gcccttcggc atggcgccca
     4381 ccttcttctc gtcgtcgtag atcaggcaca ggtaccgcat ggcttcctcc ggaaacgtgg
     4441 tcgagagtcg tggtcgccaa gtagtcgctc gggggtgggc gaaatcgaca acccgcctcc
     4501 tcgtcggcta tcccgttggc ctcatgtgat ttattcggcc ggcgtgcggt cgacgaggta
     4561 ctcgctctgg accaggccgc tgggaaaggc gcgcgtggcc gcgtggcggc ccacgatgtc
     4621 gcgcgacagc gggccgaaca gcggaatccc gctgccgatc agcacgggca cgcgtgtgat
     4681 gacgaggcgt tggatcagcc cggccgccag gaagcgctgg atcgtcacgc cgccgtcgac
     4741 gtagatgtgc tccatgccgt gcgcggcgag gcgggaaacg atctcggccg gcgtgcccga
     4801 cattcgctcg atcacggcgc ccgggaacgc cgggatgtcg agcgggcgac tgctgagcac
     4861 cacgacgggt ttcgtgccgt agggccagcc gtcgaaggtc aggacgacct cgaaggtccg
     4921 gcggcccatg acgacggcgt ccacgctcgc caggaattcc gtgaatccgt gatcctctcc
     4981 acctcccgcc ggcaagaagt cgagggcatc gttgctgcgg gcgatgaagc cgtcgaggct
     5041 cgtgccgcag aatacggagg ctttcatgcc ggtcgtgtct gcctcagaag acgaagaact
     5101 cgaatcccgt gccgaggccg agacgcgtgc cggtggcggt cacggtgaac ttgatggggg
     5161 ccagccggat tgaagatcga ttggtcgctg gtcacatagg aaccggcgta gtacacctct
     5221 ccatagatac cgaaactgtg cgtcagcaga cgaacggctc cggcgcccag cgaccagttc
     5281 cacgcgcgac ttgtcgatcg cgcatcgctg gcgccagggt catacagctt ggtcgcgctg
     5341 cgactcgccc cgaccgaaat gtaaggatag aggacgtgag gtccatgccg gaagtaatag
     5401 gtgaggccgg gcccgaatcc gtacgagaac gagtgtcccg aggaaaagtt ggtgtagccg
     5461 aaatacccga agccgcgcac ggcgacaccc ggcgagatga aatacccgag gtcgggcgac
     5521 accgagagac tggtcgttgc ggagcccgtc tccgagaccc tgctgtagga gagtccgagt
     5581 ccgccaccgg gctgccaaac cccacggtcc accgcatacg tctgggctgg cgctgccttc
     5641 acggcaacga gcagcgcgag agcagggaaa aaacgtggcg tgatggcgag cataaggggc
     5701 tgagcgggca tcaagctgca gtcaagtcgc cggcgggccg gtcagggcac gcttgagcgc
     5761 cgccaatggc aggtgcgcgg tcaccggcga gtgccgcggt cggcgacgtg tacgccaaac
     5821 tgagcagtga cgaacgccca gatgtcggtc cactcgccga tcgccgcctc ctgcgatgcg
     5881 tacatgtggc tcgcgttggg cgtcacgtgc agcaggatcg gccggctgca gcgctgcacc
     5941 ccctgcagcg ctgcggcgaa cttgtacgag tgactcggca ccacggtctc gtcgtgatcc
     6001 gccgtgagga gcagcgtggc cgggtagcag acgttcggcc gcacgtgttg gagcggggag
     6061 taggcgagca gatagcggaa cgcgaccgag tcgtcgggag agccgtactc cgtggtccag
     6121 ccggcaccga ccgtgaaccg gtgatagcgc agcatgtcgt gatgcccggc gctcggcagc
     6181 gccactccga agaggtccgg tcgctgcgtc agggcggcgc cgatcagcag cccgccgctc
     6241 gagtggccgt acacggcgag tttcgcgggg acggtatacc gccggccgac caggtattcc
     6301 gccgctgcca gaaaatcgtc gatgctgttc tgcttgtgct cgaggttgcc cgcgcggtgc
     6361 cattgctcgc cgtactctcc gccgccgcgg atgttcgcca cggcatacac gccgcccagc
     6421 tcgagccaga gcggaaggtc ggggcggtag ctcgggccga ccgtgatgcc attggcgccg
     6481 tagctcatga gcagcacggg attccgccca tcgaggcgca cgcccttttt cgcggtgatg
     6541 aacatcggta cgcgcgtgcc gtccctggag cggtagaaca cctgacgggt cccgtagggg
     6601 cgcgggtcga agggcacccg cggcggccgg aacggcgtgt tcgtcccggt ccgcagatcg
     6661 taatagtaga cggtctccgg ggagagaaac gatctgaacg agaacagcac ctcgggcgcc
     6721 gagctgcggc cgttgaccgg ccacccgagg gcgccgatgc cgggcaacgc cacttcgccg
     6781 accggccggc catcgagcgt gaacagctcc agccggctct tcacgtccac caggtagtgc
     6841 agcgcgaggc gatcgccggc gacggtcgcc cactgaatca cgtcgggaga ttcgggcact
     6901 accccacgcg gccgcgctcc ctcgccggcg ctgaggtcga gcgcgatcac gcgcccccgc
     6961 ggcgcgtcga gatcggtgaa gacgtacagc gtgtcgccca ccgtccccat cggggtgtac
     7021 cgcgcctcgc ggctcgcgag cagcggaacg aggggagccg agagatcggg cgccttcgga
     7081 tcccccaggt ccatcacgtt catccaggag gcggacccct tctcctccac gaggaacgcc
     7141 cgtcgcccgt cgtcgctcaa catgcagtac aggtagcggg cgtccgtcca ctcgtgaatc
     7201 atccggtccc gttgctgcgg cgagccgagc gtgtgataga ggtactgttt ctcgagccgc
     7261 gcggcggtga cgtcctcgcc gggccgcgcc ggccgcggac gccaatagaa gaaaccgttg
     7321 ccgtcgaacg tccagcagac cgaccccctc gtatcgcgca ccacgtcacc ctgctcacgt
     7381 cccgtggcga ggtcgcgcac atgcgtgtcc ccgcccgagc cgccgggcgc ctccgagtac
     7441 gcgagccagc gtccatcggg cgacaccgag tagtcgccca cggcgatcga tccgtccggt
     7501 gagatgtcct gggggtcgag gaccacgcgc gccgagtcgc cggggccctt caccgtgtag
     7561 aggagcggct gcggctgggc gccattgttc tcggtgaaga acagccggcc ggcttcgcgc
     7621 catggaacgt cggtgcggct gtagttccag agggccgcga gccgcccgcg gatgcgctcc
     7681 cggccgggaa ggcgggcgag gtagtcatcg gtcaaccgac gctgcagggt cacccactcc
     7741 accgtggcgg gaccgtcgag ctgctcgagc cagcgaaagg gatcggcgat cggggtgccg
     7801 aagagcgtat ccacgacggc gtcgcggtgc ggcgcggggt aagcgagcgc ctgcgcgcgt
     7861 gcaacctgca cccaggctgc cgcgctgagc agcacgatgc tccaggagcg ggctgcgcgg
     7921 cacgctgctc gtgacctcat tcgggttctc acgcggggat gattcgcctg ggacgaatcg
     7981 tggagcaggc gttgcggaac cgtgcgatca cttcggtgac gtcccggtag ctctcggttt
     8041 ctctcaggtc ctcgcggtac aggacgcgct cggccaccgg cgccaggcgg aaacaccaat
     8101 agatgggcat gaaagggttg atccacagct cgctgaattc agtccggggg gtggcgtggt
     8161 ggtcgccata gtaaccggcc agcgccgaca ggatggagct cgacacgata ctgatatggt
     8221 gcggcatcgc cgcgaaaacc gcctcggtcg ccagccggt
//
LOCUS       DBNGZW010000528         2279 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1401054, whole genome shotgun sequence.
ACCESSION   DBNGZW010000528 DBNGZW010000000
VERSION     DBNGZW010000528.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1401054"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2197
                     /locus_tag="AC16JC_12585"
     CDS             <1..2197
                     /locus_tag="AC16JC_12585"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HIR5165578.1"
                     /translation="IGRRDEFPQSDRVILLIDSYHDRRTGFEFGVTAAGGRYDAAIYD
                     DNNEDPAWDGVWDVATSIDSAGWTAEFRIPLSQLRYGSERRHTFGLAIDRDLYRYNER
                     LAWPLFRQSAAGMVSQFAELTGLDDLEAPRRLEAAPYVVTKNVSRVEAAGFGREQKVS
                     LGADLKYRVASNLTLDATANPDFGQVEADPGVLNLTAYETFFPEKRPFFVSGRGLFDV
                     SINCNNVNCNSERLFYTRRIGRSSATILGAAKLTGRVANGLRVGLLDAVTRKTTAAGG
                     ETLEPAANYGVFRLDQDFDAGEGTVGVIATAVNHGLDVWSRPSVPASSYAGAVGFRQR
                     LFKQTYQISALLSGSRVSGSAPVISEIQRSPVHYYQRPDAGLPYDTTRTVLAGDAEEL
                     KFAKVAGRHTQFETSWQRRSPGFEINDLGYLQRAGAHNWSTWVGFFDRNVRRLYQSFQ
                     WNWNWWQIWTTEGLPLERAANTNVHVTLRNNWGIHFGGTWGQLGATYDDRAARGGPAM
                     RQDSYLAPWLYINGDDRQQLVPTLSANVFRSGDGHGRSLSVSPGVSVKLSTRLLATAS
                     LDWSKNDNGYQWYGNVTDTLGETHYGFARLRQTTEAITTRVNYTISPVMTLQVYAQPF
                     VSKGTFSDLRELSATPRAARYDDRFQPYFDPAVAADPGGFRYMQFRSSVVFRWEYKPN
                     SILFVVWTQGRQGLADQEGTAGFTGDLNELFDLRPDNTFLLKLSYWINR"
BASE COUNT          378 a          801 c          735 g          365 t
ORIGIN      
        1 tatcgggcgc cgcgacgagt ttcctcagtc cgaccgggtc atcctgctga tcgactcgta
       61 tcacgatcgc cgcaccggct tcgagttcgg cgtcaccgcg gccggcggac gctacgacgc
      121 ggcgatctac gatgacaaca acgaggaccc cgcatgggac ggggtctggg acgtggccac
      181 gagcatcgac tcggccggct ggacggcgga gttccgcatc ccgctgtcgc agttgcgtta
      241 cgggtcggag cgacggcaca ccttcgggct cgccatcgac cgcgacctgt accgctacaa
      301 cgagcggctc gcctggccgc tattccgcca gtcggcggcg gggatggtct cgcagttcgc
      361 cgagctgacg ggcctcgacg acctcgaagc cccgcggcgc ttggaggcgg cgccgtacgt
      421 cgtcaccaag aacgtgtcgc gcgtcgaagc ggcgggcttc ggccgcgagc agaaggtctc
      481 gctcggcgcc gatctgaaat accgcgtggc ctcgaacctg accctcgacg ccacggccaa
      541 ccccgatttc ggccaggtcg aagccgatcc cggtgtgctc aacctcacag cctatgagac
      601 cttcttcccc gagaagcgcc cgttcttcgt ctcgggccgc ggcctgttcg acgtctccat
      661 caactgcaac aacgtcaact gcaacagcga gcggctgttc tacacccgtc gtatcggtcg
      721 atcctccgcc accatcctcg gcgccgccaa actcaccggc cgcgtcgcga acgggctccg
      781 ggtcggcctg ctcgacgcgg tcacccgcaa gacgacggca gcggggggtg agacgctgga
      841 gccggctgcc aactacgggg tcttccgcct cgaccaggat ttcgacgcgg gggagggaac
      901 cgtcggcgtc atcgccaccg cggtcaatca cggcctcgac gtctggagcc ggccatcggt
      961 gccggccagc tcctacgccg gtgcggtggg cttccgccag cgactcttca aacagaccta
     1021 ccagatctcg gccctgctgt cggggagccg cgtatcgggc agcgccccgg tcatctcgga
     1081 gatccagcga agcccggtgc actactatca gcgaccggac gccggcctgc cctacgacac
     1141 gacccggacc gtcctcgcgg gcgacgccga ggagctgaag ttcgccaagg tggcgggacg
     1201 gcacacccag ttcgagacct cctggcagcg ccgctccccg ggcttcgaga tcaacgacct
     1261 gggatacctg caacgggccg gcgcgcacaa ctggtccacc tgggtcgggt tcttcgaccg
     1321 caacgtgcgc cggctgtatc agagcttcca gtggaactgg aactggtggc agatctggac
     1381 gacggagggc ctgccgctcg agcgggcggc caacaccaac gtgcacgtca ccctgcggaa
     1441 caactggggc atccatttcg gcgggacgtg ggggcaactc ggcgccacct acgacgaccg
     1501 cgccgcgcgg ggcggacccg cgatgcggca ggactcctat ctcgcgccgt ggctctacat
     1561 caacggcgac gaccgccagc agttggtgcc gactctctcg gctaacgtct tccggagcgg
     1621 cgacggtcat ggccgttccc tctcggtgag ccccggggtg agcgtcaagc tgtccacccg
     1681 cctcctcgcc acggcctccc tcgattggtc gaagaacgac aacggctacc agtggtacgg
     1741 caacgtcacc gacacactgg gggagaccca ttacggattc gcgcggctgc gccagacgac
     1801 cgaggcgatc acgacgcggg tcaactacac gatctcgccc gtcatgacgc tccaggtgta
     1861 cgcgcagccg ttcgtttcga aggggacgtt cagcgacctg cgcgagctgt cggccacacc
     1921 gcgggccgcg cgctacgacg accggttcca gccctacttc gacccggcgg tggcggccga
     1981 tccgggtggg ttccggtaca tgcagttccg ctcgagcgtc gtgttccgtt gggagtacaa
     2041 gcccaactcg atcctgtttg tcgtctggac gcagggccgc cagggtcttg cggaccagga
     2101 aggcaccgcc ggtttcacgg gcgatttgaa cgagttgttc gacctgcggc cggacaacac
     2161 gttcctgctg aagctctcgt actggatcaa ccgctaggag tctcggccta ccactgggcc
     2221 ctcgcgtgca tcgccgtggc gaagacctca cggcgcgcgg gcgaccgtga actcgtagc
//
LOCUS       DBNGZW010000529         2139 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_662081, whole genome shotgun sequence.
ACCESSION   DBNGZW010000529 DBNGZW010000000
VERSION     DBNGZW010000529.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2139)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2139)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2139
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_662081"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1219
                     /locus_tag="AC16JC_12590"
     CDS             77..1219
                     /locus_tag="AC16JC_12590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165579.1"
                     /translation="MTSQEPRVVGSVVLYRLYDVGYEIDLERALDFLATTPEEREALR
                     LKLERRQRAEAQAIRIKNPPVTVTLGSEQFDAAGRRYDASVSARIFDFGVISLRMRCG
                     IPGALPWSAFAAFGDAAELSAAARPAFDRRIRELTERIAPAVARLQIAPVSEDYVVYR
                     ITRLESPSGEPLLASAVRELDVAPLLLNEDRALSDQARKELLPHWFSYYPDDLSIVTW
                     NTALVLDPAEGESDEQLILEFANAQLLELRYYDALLDAELPQMYDRIASARKGAGGLL
                     GRRYAPLLTALQTLVADSTEIVERTQNALKVTDDIFLARVYAAALEVFRERAWRTGID
                     HKLDIIRQTYGMLNAESMAARNGLLEIAVVVLILVEIVIAVVLGGH"
     gene            complement(1241..1738)
                     /locus_tag="AC16JC_12595"
     CDS             complement(1241..1738)
                     /locus_tag="AC16JC_12595"
                     /inference="COORDINATES: protein motif:HMM:NF012451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HIR5165580.1"
                     /translation="MTIDPSQAYRLLGLAPGASFEDVKTAYRDLAQVWHPDRFPDNAR
                     LREKAEVNLRRINEAYTVLRDYRAGAASAAVVATAPAPHAAARAATASRQDPPLHFDL
                     RHSMFTGVVAMRHSIKVLRPSATYARRPRRRGLPKPWIRLGIATALLVALAVAALWYK
                     GYLDF"
     gene            1886..>2139
                     /locus_tag="AC16JC_12600"
     CDS             1886..>2139
                     /locus_tag="AC16JC_12600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165581.1"
                     /translation="MPFHRCAIHKDDGSTVAASVDVMIEELERPGGNEWHGTISTTHL
                     VTLEAGHRYKLVLDDGRSGEFMVRRNTFAGGTNRAVAIQG"
BASE COUNT          329 a          706 c          764 g          340 t
ORIGIN      
        1 cggctgtact tcaccctcgg cgatctgcag agcgatatct gggtgaccga cctggagccc
       61 cgatgaccgt ccggtggtga cgagccagga accgcgcgtc gtcggttccg tggtgctgta
      121 ccggctgtac gacgtgggct acgagatcga tctcgagcgt gccctcgact tcctcgcgac
      181 gactcccgag gagcgggagg cgctgcgctt gaagctcgag cggcggcagc gcgccgaggc
      241 gcaggcgatc cggatcaaga acccgccggt cacggtgacg ctcggcagcg agcagttcga
      301 tgccgccggg cgccgctacg acgcctccgt ctccgcccgc atcttcgact tcggcgtcat
      361 ctcgctgcgg atgcggtgcg gcatcccggg ggcgcttccc tggtccgcct tcgccgcgtt
      421 cggcgacgcg gcggagctgt ccgcggcggc ccggcccgcg ttcgaccggc ggatccgcga
      481 gctcaccgag cgcatcgcgc cggcggtcgc gcggctgcag atcgcgcccg tgagcgaaga
      541 ctacgtcgtc taccggatca cgcgtctgga gagcccctcc ggcgagccgc tcctggccag
      601 tgcagtgcgc gagctcgatg tggcgccgct gctcctgaac gaggaccggg cgctctccga
      661 ccaggcgcgc aaggagctcc tgccgcactg gttttcctac tacccggacg acctttcgat
      721 cgtcacctgg aacaccgccc tggtgctcga cccggccgag ggggagagcg acgagcagct
      781 gatcctcgaa ttcgccaacg cgcagctgct ggagctgcgc tattacgacg cgctgctcga
      841 cgccgagctg ccgcagatgt acgaccggat cgcgagcgcg cggaagggag cgggcggtct
      901 gctgggtcgg cgctacgcgc cgctgctcac cgcgctccag acgctggtcg cggactcgac
      961 cgaaatcgtg gagcggaccc agaatgcgct caaggtcacc gacgacatct ttctggcccg
     1021 cgtgtatgcg gcggcgctgg aggtgttccg ggagcgggcg tggcgcaccg ggatcgacca
     1081 caagctggac atcatccggc agacgtacgg gatgctcaac gccgaatcga tggcggcgcg
     1141 caatgggctg ctggagatcg cggtggtggt gctgattctg gtcgaaatcg tgatcgcggt
     1201 ggtgttgggc gggcattgat cgacgcccac ccggagcgcc tcagaagtcc agatacccct
     1261 tgtaccacag cgccgcgacc gcgagcgcca cgagcagcgc ggtggcgatc cccaggcgga
     1321 tccacggctt ggggagcccg cggcggcgcg gtctgcgcgc gtaggtggcg ctcggccgca
     1381 gcaccttgat cgagtggcgc atcgcgacga cgccggtgaa catcgaatgc cgcagatcga
     1441 agtggagcgg cgggtcctgt cggctcgccg tcgcggcgcg cgccgccgcg tggggggccg
     1501 gcgccgtggc gacgacggcg gccgatgcag caccggcccg gtagtcccgc agcaccgtgt
     1561 acgcctcgtt gatgcgccgc aggttcactt cggccttctc gcgcagccgg gcgttgtcgg
     1621 ggaaccggtc gggatgccac acctgagcca ggtcccggta cgcggtcttc acgtcttcga
     1681 acgacgcgcc gggggcaagc cccaggagac ggtaggcttg ggagggatcg atggtcatgt
     1741 gttctggtga cggcctcggt cgcaagactc gtgccggaac tcgcgtgctg gcgacggtcg
     1801 aatgaagtat gggcaggtcc gggcaatgac gccagtcggc gatggggttg ttgcagggtg
     1861 tcgccaatgc cgcaatcttt gcagcatgcc ctttcatcgc tgcgcgatcc acaaggatga
     1921 cggtagtaca gtagccgcgt cggtcgacgt catgatcgag gagctggaac gtcccggcgg
     1981 gaacgagtgg cacggcacga tctccaccac gcacctcgtc accctggaag cggggcaccg
     2041 ctacaagctc gtgctcgacg acggccgcag cggcgaattc atggttcgcc gcaacacgtt
     2101 cgcgggcggc acgaatcgcg ccgtcgccat tcaggggat
//
LOCUS       DBNGZW010000530         3598 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1059501, whole genome shotgun sequence.
ACCESSION   DBNGZW010000530 DBNGZW010000000
VERSION     DBNGZW010000530.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1059501"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="AC16JC_12605"
     CDS             <1..951
                     /locus_tag="AC16JC_12605"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR5165582.1"
                     /translation="SLTKVIGLTTACMILVDEGKLDLDAPVRAYVPQFRGPQKDQVTI
                     RQLLTHSAGMHWWRPLYREATDRNSAITLVDTAPLDTTPGTRFVYSDLGAILLMQVVE
                     RITGERIDHYLAGHVFGPLGMTSTRYRPPRSWQSRIAPTEIDTVFRHRLIRGEVHDEN
                     AGRMDGVSGHAGLFSDAPDLSRFASWLLKTYQTEQGARSSTLTASSAPPAPCSVLCFS
                     SDIVTRFTHRQGVPPGSTRALGWDTPSDSGYSSAGTKLSRASFGHTGFTGTSIWMDPE
                     RDLFVILLTNRVNPTRANTKILQVRATVADLVAAALVANP"
     gene            1035..1394
                     /locus_tag="AC16JC_12610"
     CDS             1035..1394
                     /locus_tag="AC16JC_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR5165583.1"
                     /translation="MTTTQKPESITLTLTERAAVEVRKFMADEKVDPAIAGLRVGVMP
                     GGCSGFKYQLNIEDKAADDDLTIESSGVRLFVDAFSATYLNGVTIDYKSNFQESGFAF
                     DNPNATGGCGCGSSFTV"
     gene            1476..3023
                     /locus_tag="AC16JC_12615"
     CDS             1476..3023
                     /locus_tag="AC16JC_12615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HIR5165584.1"
                     /translation="MRPLDIAVIVAYCVAVAAVGVLLAGRQRDSTDYFLGRHGLPWWA
                     ILLSIVATETSALTVISVPGLAATGKLTFIQLAFGYLVGRIGVAAILLPGYFEGTQDT
                     AYQRLERRFGPHARRAASGVFLVTRALADAVRVFATAIPLAIITHWSIPAGILATGVI
                     TLAYTWFGGIRAVVWTDILQLGVYILGGVATLIVALGRVGGVSAFGTAYDHFKLVFLD
                     FSFDFTHTYTLWSGLIGGALLSAASHGTDHLIVQRLLAARGLRDARKALIGSGVFIIF
                     LFALFLLVGTSLWLAGAAHPGVPGDQVYPGFVATQLPAGLAGLVVAGLLAAAMGTHAS
                     AINSLASASTNDFYAPLTGQRDPTRLLKVGRVMTLVWAVILVAGAMLFPDRRTPVVEL
                     ALQVASLTYGALLGTYLLGGFWPRARERDVIVAMVVGVIVMVPVIFAGRLAGLLHNPG
                     WLVSLSHLAWPWDVPLGTVVTVAVGVASSFMGRPAAALPTADTTTAHRTTGDKATDDA
                     ATDTPAP"
     gene            3034..>3598
                     /locus_tag="AC16JC_12620"
     CDS             3034..>3598
                     /locus_tag="AC16JC_12620"
                     /inference="COORDINATES: protein motif:HMM:NF013986.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HIR5165585.1"
                     /translation="MTAGSSSAATSDVVSPTVLLGVDVGGSHTAAAISRADFVVLSRA
                     EGPGVPMRSGGASETAAAIAEVTVRAARVAGVTLPVDRAMVGAAGAGRAMERRELKQA
                     LAAHGVARSLEVVADGEIALAAAFGTEPGILVNAGTGSIAYARDRHGRLRRSGGYGWQ
                     LSDEGGGYWIGRRALSVAAKARDGSGDG"
BASE COUNT          513 a         1311 c         1175 g          599 t
ORIGIN      
        1 tccctcacca aggtcatcgg gctgacgacg gcctgcatga tcctggtgga cgagggcaag
       61 ctcgatctcg acgcgccggt gcgagcgtac gtgcctcaat tccgcggacc gcagaaggac
      121 caggtcacga tccgtcagct gctcacccac tccgccggga tgcactggtg gcgcccgttg
      181 tatcgcgaag cgacggatcg aaacagcgcc atcaccctcg tcgacacggc gccgctcgac
      241 acgacaccgg gcacgcgctt cgtctattcc gatctcggcg cgatcctgct catgcaggtc
      301 gtcgagcgca tcacgggtga gcggattgat cactacctcg cgggacacgt gttcggcccc
      361 ctcggcatga cgtcgacccg ctatcgccca ccgcggagct ggcagagccg gatcgcgccg
      421 accgaaatcg acaccgtctt ccgccaccgc ttgatccgcg gcgaggtgca cgacgagaac
      481 gcgggacgca tggacggagt gtcgggacac gcgggattgt tcagcgacgc gccggacctg
      541 agccggttcg cgagctggct gctcaagacc taccaaacag agcagggagc acggagcagt
      601 acgctcacag catcgagcgc gccccctgca ccgtgctccg tgctctgctt ttcgtcggac
      661 atcgtcaccc ggttcacgca tcgacagggc gtccctcccg gctccacccg cgccctgggc
      721 tgggacacgc cgtcggattc gggctactcg agcgccggaa cgaagctgtc acgggcgtcg
      781 ttcgggcaca cgggcttcac cggcacgtcg atctggatgg accccgagcg cgacctgttc
      841 gtcattctgc tcacgaatcg cgtgaatccg acccgcgcga acacgaagat cctgcaagtg
      901 cgcgccacag tggcggacct cgtggccgca gcgctggtcg ccaacccgta ggccgcccct
      961 gtccgccctc gtccgcccgc ctctttcgct ctcttccgcc cctgtttagg ttattgcctg
     1021 acggaggaag cgacatgacc acgacccaga agcccgagtc catcaccctc accctcaccg
     1081 agcgtgccgc ggtcgaggtg cggaagttca tggccgacga aaaagtggac cccgcaatcg
     1141 cgggtctgcg cgtcggcgtg atgccgggtg ggtgctcggg attcaagtat cagttgaaca
     1201 tcgaggacaa agccgccgat gacgatctga cgatcgaatc gagcggtgtg cgcctgttcg
     1261 tggacgcctt cagcgccacc tacctcaacg gcgtgaccat cgactacaag agcaacttcc
     1321 aggagtccgg cttcgcgttc gacaacccga acgccaccgg cggctgcggc tgcggatcgt
     1381 cgttcacggt ctagccccgc tcccgttttc caccgtagct tccgaggccc gcccacccgc
     1441 gggcctcggt tcgtttgcca ccccgggagg agcgcgtgcg accgctcgac atcgccgtca
     1501 tcgtagccta ctgcgtcgcc gtcgcggccg tcggcgtcct gctcgccggc cgccagcgcg
     1561 acagcaccga ctacttcctc ggccgtcacg ggctgccctg gtgggcgatc ctcctctcga
     1621 tcgtcgccac cgagacgtcg gccctcaccg tgatctcggt cccgggcctc gccgccaccg
     1681 gcaaactcac cttcatccag ctggcgttcg gctatctcgt cggccggatc ggcgtcgccg
     1741 ccattctcct cccgggctac ttcgaaggca cccaggatac ggcctaccag cggctcgagc
     1801 gccgcttcgg accccacgcg cgccgtgcgg cctccggggt gttcctggtc acgcgtgcgc
     1861 tcgccgacgc cgtacgcgtg ttcgccaccg ccatcccgct ggcgatcatc acgcactgga
     1921 gcatccccgc cggcattctg gcgaccggcg tcatcaccct ggcgtacacc tggttcggcg
     1981 ggatccgggc tgtcgtctgg accgacatcc tgcagcttgg cgtctacatc ctggggggcg
     2041 tcgccacgct gatcgtggca ctgggccgcg tgggcggcgt gagcgcgttc ggcaccgcct
     2101 acgaccactt caagctggtg ttcctcgact tcagcttcga tttcacccac acctacacgc
     2161 tgtggagcgg gctcatcggc ggcgcgctgc tctcggccgc ctcgcacggc accgatcacc
     2221 tcatcgtgca gcggttgctc gccgcgcgcg gactccgcga cgcccgcaag gcgctcatcg
     2281 gctccggggt gttcatcatc ttcctgttcg ccctgttcct gctcgtgggc accagtctct
     2341 ggctcgcggg cgccgcccac ccgggcgtgc cgggagacca ggtctacccg ggcttcgtcg
     2401 ccacacagct cccggcagga ctggccgggc tcgtcgtggc cggcctgctc gccgcggcga
     2461 tgggcacgca cgcctcggcg atcaactcgc tcgcctccgc gtccaccaac gatttctatg
     2521 ctccgctcac cggccagcgc gatcccacac ggctgctcaa ggtgggacgg gtcatgacgc
     2581 tggtgtgggc cgtgatcctg gtggccggcg ccatgctctt tcccgaccgg cgcacgccgg
     2641 tggtcgagct ggcgttacag gtcgcttcgc tcacctacgg cgcgctgctc ggcacctacc
     2701 tgctgggagg gttctggccc agggcgcgcg agcgcgacgt catcgtcgcg atggtcgtgg
     2761 gcgtgatcgt gatggtgccg gtgatcttcg cgggccggct ggcgggcctg ctgcacaatc
     2821 ccggctggct ggtgtcgctc agccatctcg cctggccctg ggacgtcccg ctcgggacgg
     2881 tcgttaccgt cgcagtcgga gtcgcatctt catttatggg ccggccggcc gccgcgctgc
     2941 cgacggcaga taccacgacg gcacacagga cgacggggga caaggcgacg gatgatgctg
     3001 cgacggacac ccccgcccca tgacttccgc ttcatgaccg ccggttcatc gtccgccgcc
     3061 acatccgacg tcgtctcacc caccgtcctc ctgggcgtcg acgtcggggg aagccatacc
     3121 gccgcagcca tctcccgagc cgatttcgtc gtgctgagtc gtgccgaggg ccccggagtc
     3181 ccgatgcgct cgggcggcgc ctcggagacc gccgcggcaa tcgccgaagt cacggtgcgt
     3241 gccgcccggg tggccggggt gaccttgccc gtggatcgcg cgatggtggg agcggccggc
     3301 gcgggccgcg ccatggaacg ccgcgagctc aagcaggcgt tggccgcgca tggcgtggcc
     3361 cgctccctgg aggtggtggc cgacggagag atcgcgctgg cggcggcgtt cggcaccgag
     3421 ccgggcatcc tcgtgaacgc cggcaccggc tcgatcgcct acgcgcggga ccgccacggg
     3481 cgcctgcgcc gcagcggcgg ctacgggtgg cagctgagcg acgaaggcgg gggctactgg
     3541 atcgggcgac gcgcgctgtc ggtcgcggcc aaggcgcgcg acggcagcgg ggacggcg
//
LOCUS       DBNGZW010000531         1959 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_867345, whole genome shotgun sequence.
ACCESSION   DBNGZW010000531 DBNGZW010000000
VERSION     DBNGZW010000531.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1959)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_867345"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="AC16JC_12625"
     CDS             <1..523
                     /locus_tag="AC16JC_12625"
                     /inference="COORDINATES: protein motif:HMM:NF015431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HIR5165586.1"
                     /translation="CREGESDRLLADLSLHALDLVLLDAPAGSAIKARVFSHPLGECG
                     VTIFGAPALAGQVRRRFPRSLDAAPFLLPGAGTMLRQSLELWMEGEGIRPAVVGEIGD
                     SAVLKTFGGAGAGLFAAPSAVERDVCRQYQVRVAGRLPKIRERFYAVSVERRLKHPAV
                     VAITETARTELFS"
     gene            complement(527..1045)
                     /locus_tag="AC16JC_12630"
     CDS             complement(527..1045)
                     /locus_tag="AC16JC_12630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165587.1"
                     /translation="MYVTLFTLALAAAPAAAEAPRPDRHAATRQAEAHEALIERHRQA
                     RHAAGALYQFAASRVVADRELAQRMAVDISSSLDTGQQEIDEIAELLAPIEREGANAP
                     LQELRDRQAQAVSHVGALKSALAAAPIDGRQVRFLAGQIYEAVGQAHDTHQALMRQLN
                     VKVADRSAPRVR"
     gene            complement(1145..1483)
                     /locus_tag="AC16JC_12635"
     CDS             complement(1145..1483)
                     /locus_tag="AC16JC_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012527565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HIR5165588.1"
                     /translation="MSQPVIPCACARPGDGTHAVAVDPEIKRAVLTRLRRIEGQIRGL
                     QKMVDEERYCADVLTQVSSVQEALRGVSRSLLQNHLKHCAAEAIRSNDPERSDAMYEE
                     LLELVFRSGR"
     gene            complement(1480..>1959)
                     /locus_tag="AC16JC_12640"
     CDS             complement(1480..>1959)
                     /locus_tag="AC16JC_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013490059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR5165589.1"
                     /translation="RTAAVIAKEAGVDDFLAEATPEDKMELIKSEQRGGHLVAMTGDG
                     TNDAPALAQADVGIAMGTGTDVAMEAGAITLMRGDPMGVVTAIRLARRTMRVIRQNLF
                     WAFIYNVVGIPVAAGVLYPGFGLRLTPAMAAAAMAVSSVSVISNSLRLRRFNSKGQP"
BASE COUNT          263 a          680 c          686 g          330 t
ORIGIN      
        1 ctgtcgggaa ggagagtcgg accgactgct cgccgatctg agcctgcatg cgctcgatct
       61 cgtgctgctc gacgcgccgg cgggcagtgc gattaaggcg cgcgtcttca gccatccgct
      121 gggggagtgc ggggtcacga tcttcggtgc tccggcgctc gccggtcagg tccggcggcg
      181 ctttccccgc tcgctggacg ccgccccctt tctcctcccc ggcgccggga cgatgctgcg
      241 gcaatccctg gagctctgga tggaagggga aggcatccgg ccggctgtcg tgggggagat
      301 cggagacagc gcggtgctca agaccttcgg cggggcgggc gcgggcctct tcgctgcgcc
      361 gagcgcggtg gagcgggacg tctgtcgcca gtaccaggtt cgggtggccg gccgcctgcc
      421 gaagatccgt gagcggttct acgccgtgtc agtggagcgc cgcctcaagc atcccgccgt
      481 cgtggccatc accgagacgg cgcgcaccga gttgttcagc tagtcatcac cgcacgcgcg
      541 gcgcgctgcg gtcggcgacc ttcacgttca gctgccgcat gagcgcttgg tgcgtgtcgt
      601 gcgcctgccc caccgcctcg tagatctggc ccgcgaggaa ccggacctgg cggccgtcga
      661 tcggcgctgc ggcgagcgcg ctcttgagcg cgccgacgtg gctgacggcc tgtgcctgcc
      721 ggtcgcgcag ctcctgcagg ggcgcattcg cgccctcgcg ctcgatcggt gcgagcagct
      781 ccgcgatctc atcgatctcc tgctggcccg tgtcgaggct gctactgata tccacggcca
      841 tccgctgtgc gagctcgcgg tccgcgacga cgcgcgaggc ggcgaactgg tagagcgcgc
      901 ccgcggcgtg acgggcctga cggtggcgct cgatgagggc ctcatgcgcc tcggcctggc
      961 gcgtggcggc gtggcggtcc ggccgggggg cctccgctgc ggcgggcgcc gcggcgaggg
     1021 cgagggtgaa gagcgtcacg tacatggtcc tcctcctggg ttgaagtgac gccaccagtc
     1081 taggaggcaa tccggcgcgg gacaatttca acttttgcgt gacaatccac gatttttccg
     1141 acccttaacg gccgctccgg aacaccagct ccagcagctc ctcgtacatc gcatccgacc
     1201 gctcgggatc attcgaccgg atcgcctccg cggcgcagtg cttcaaatgg ttctgcagca
     1261 gcgaccggct cactccacgc agggcctcct ggaccgacga cacctgggtc aggacgtccg
     1321 cgcagtagcg ctcttcgtcc accatcttct gcaggccgcg gatctggccc tcgatccggc
     1381 ggagccgcgt cagcaccgcg cgcttgatct ccggatccac cgccacggcg tgcgtgccgt
     1441 cgcccgggcg ggcgcaggcg caagggatca cgggctggct catggctgtc ctttcgaatt
     1501 gaagcggcgg agccggagac tgttggagat cacgctcacc gagctcactg ccatggccgc
     1561 cgcggccatc gcgggggtga ggcggagacc gaaaccgggg tagagcacgc cagccgcgac
     1621 cgggatgccg accacgttgt agatgaacgc ccagaacagg ttctgccgga tcacccgcat
     1681 ggtccggcgg gcgagccgga tggccgtcac gactcccatc ggatccccgc gcatcagcgt
     1741 gatggctccg gcttccatgg cgacgtcggt tccggtcccc atggcgatgc cgacgtccgc
     1801 ctgcgccagc gcgggcgcgt cgttggtgcc atcgccggtc atcgcgacga ggtggccgcc
     1861 gcgctgctcc gacttgatga gctccatctt gtcttcgggc gtcgcctccg cgaggaagtc
     1921 gtcgacccct gcctccttgg cgatcacagc cgcggtgcg
//
LOCUS       DBNGZW010000532         3571 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_187194, whole genome shotgun sequence.
ACCESSION   DBNGZW010000532 DBNGZW010000000
VERSION     DBNGZW010000532.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3571)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3571)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3571
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_187194"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="AC16JC_12645"
     CDS             <1..478
                     /locus_tag="AC16JC_12645"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5165590.1"
                     /translation="TIGSPPARAGVVRWVNDSLLQRRRPVALDARLRRVVSHVRSHLG
                     TGDSLSLKALAAVAGMSPSRFMHLFTQSLGVPVRPYIRWLRLQHAACELMDGATASMA
                     AHRAGFADAAHMTRTFRFMLGTTPTDLALRKRLAAGVSIESGAPTVHPLRGGVPAR"
     gene            605..1399
                     /locus_tag="AC16JC_12650"
     CDS             605..1399
                     /locus_tag="AC16JC_12650"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018413293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5165591.1"
                     /translation="MAITSLAGKWALVTGASSGFGVEFATLLAEQQANLVLVARRTDR
                     LTQLGAQLESRYRIQTVVEGVDLSAPGAGAALKRRLDAQGIDVAILVNNAGYGLYGSL
                     VDQPLERTLDMLQLNIVALTELTRLFAADMVGRGSGHIILVASILGYQGVPGYAAYAA
                     SKAYVLQFGEALHEELRPHGVTVTVLSPGVTATAFGDVAGQRDTGPLRFLMMPPRPVA
                     RIGVAALLRRRSSVIAGAWNNLIVFSNRFTPRRLQRLIMQRVFAGR"
     gene            complement(1501..2001)
                     /locus_tag="AC16JC_12655"
     CDS             complement(1501..2001)
                     /locus_tag="AC16JC_12655"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR5165592.1"
                     /translation="MRTLWAAALTVGFGASCGPARLTDERRAAIVDSVEHVVHGMLDA
                     ANHRDAAGFVGAYVDEAVMAYNGVIYSTRDSYRAALDSVWQGLAGLETRPMALRTMVL
                     GPDAAVSMVPFAVTLSTTSGQQVTTQGVYTMLLQRRGNAWRVLRSHESEQHLDQVLQG
                     VMRAPR"
     gene            2129..2449
                     /locus_tag="AC16JC_12660"
     CDS             2129..2449
                     /locus_tag="AC16JC_12660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165593.1"
                     /translation="MLVRLPSPRRAEHAEHPCADPHSVFGPGPGPGRARARRGHAQTL
                     VQYGNQARLWSANARFGWLGTAGNGLYVVFNEGQAASGFFQWDRPQTRSLIVKYSRQL
                     GRIG"
     gene            complement(2478..2993)
                     /locus_tag="AC16JC_12665"
     CDS             complement(2478..2993)
                     /locus_tag="AC16JC_12665"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018212703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR5165594.1"
                     /translation="MRPVRYSVAMSLDGYIADPTGGYDWIVMDPDIDFGALFKNFDTL
                     LMGRKTYEMAREQGGGPAMRGMKSYVFSRTLRQEECPRVTVSADPKETVTALKQRQGK
                     DIWLFGGGGLFQSLLALELVDAVQVAIIPVLLGGGVPVLPSPATRAKLRLTSHRIYEK
                     TGTVLLNYAVG"
     gene            3292..>3571
                     /locus_tag="AC16JC_12670"
     CDS             3292..>3571
                     /locus_tag="AC16JC_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165595.1"
                     /translation="MSLGPEDSVDIGMLRNAPNGHISYQQAKGGLTIWVDGRIGDATQ
                     GTFILHPHSWSILELERAAPVRVFEAVHDSTLPCTSDSGWYRNYAAINA"
BASE COUNT          530 a         1294 c         1155 g          592 t
ORIGIN      
        1 aacgatcggg tcaccgcccg cccgggccgg cgtcgtccgc tgggtgaatg actccctgct
       61 gcagcgccgg cgccccgtcg cgctcgacgc gcgcctccgg cgcgtggtct cgcacgtccg
      121 cagccacctc ggcacgggcg attctctctc cctcaaggcg cttgcggcgg tggcgggcat
      181 gtccccctcc cgcttcatgc acctgttcac ccagtccctg ggcgtgccgg tgcggcccta
      241 tatccggtgg ctgcgcctgc agcacgccgc ctgcgagctc atggacggcg cgacggcttc
      301 catggcggcg caccgcgccg ggttcgccga cgcggcgcac atgacgcgga ccttccgctt
      361 catgctcggc accacgccca ccgacctggc cctgcgcaag cgcctggccg ccggagtgtc
      421 gatcgaatcc ggcgcgccga ccgtccaccc cctgcgtggc ggtgtcccgg cgcgctgact
      481 cggaagcctc actgcccgtc gacgccactt ctcgccgcgc agccagtccg ttcaagattc
      541 ctccgcggac ttcgattagc tcttcgctct tccccgacgt catcgcggag gaggcgcggc
      601 gactatggca atcacatccc tagccggcaa atgggcgctg gtcaccgggg catcgagcgg
      661 gttcggcgtc gagttcgcga ccttactcgc cgaacagcag gcgaatctcg tcctcgtcgc
      721 gcggcggacc gaccggctga cgcagctcgg cgcccagctc gagtcccgct accggatcca
      781 gacagtggtg gaaggagtgg atctttcggc gcccggagcc ggggctgcgc tcaagcgtcg
      841 cctcgatgcc caggggatcg acgtcgccat tctcgtgaac aacgcgggct acggtctcta
      901 cggcagtctc gtcgatcagc cgctcgagcg aacgctcgac atgctgcagc tgaatatcgt
      961 cgcgctgacc gagctgacgc gcctcttcgc cgcggacatg gtgggtcgcg gctcgggtca
     1021 catcatcctg gtcgcttcga tcctcgggta tcagggcgtg ccggggtacg ccgcctacgc
     1081 ggcgagcaaa gcctatgtcc tccagttcgg ggaagcgctc cacgaggagc tccggccgca
     1141 cggcgtgacg gtcacggtgc tctccccggg agtgactgcc acggcgttcg gggacgtggc
     1201 cggccagcgc gacacggggc cgctgcggtt tctcatgatg ccgccccggc ccgtggcacg
     1261 catcggcgtc gcggcgctgc tgcgccgccg gtcgagtgtc atcgcggggg cgtggaacaa
     1321 tctcatcgtg ttcagcaacc ggttcacgcc gcggcgcctg caacgcctga tcatgcaacg
     1381 ggtgttcgcc ggtcggtgac ggcgtacttg gttccccctc tccgcgtatg cggagagggg
     1441 gacaggggga gaggtacgtc tgcccgcgag ccggacaggt ggagcggtac cctgctgccg
     1501 ctaccgcggc gcccgcatca ccccttgcag cacctggtcc aggtgctgct cggactcgtg
     1561 cgaccggagc acgcgccacg cgttcccgcg ccgctgcagc aacatcgtgt agacgccctg
     1621 cgtcgtcacc tgctgtcccg acgtcgtact cagcgtgacc gcaaacggca ccatcgacac
     1681 cgccgcgtcc ggtcccagca ccatggtgcg cagcgccatc ggacgggttt ccagcccggc
     1741 aagcccctgc cagaccgagt cgagcgccgc gcgataggag tccctggtgg agtagatgac
     1801 gccgttgtac gccatcacgg cctcgtccac ataggcgccc acgaacccgg ccgcgtcgcg
     1861 gtggttggcc gcgtcgagca tcccgtgcac gacgtgctcc acggagtcga cgatcgcggc
     1921 ccgccgttcg tcggtgagcc gtgccggacc gcacgacgca ccgaacccca cggtgagagc
     1981 ggctgcccag agcgttcgca tgttcacctc catgtgcaga gcctcgtgaa catgtactaa
     2041 ctgccggcgg cgcgaatcgc caggcggcgg tattttctga gccgatgatc ggccgccgtc
     2101 tccctctcct gctcgcgctg ggcgggctgt gctcgtccgt ctcccatctc ctcgccgcgc
     2161 agagcacgca gaacacccct gcgccgaccc gcacagcgtc ttcggccccg gtcccggccc
     2221 cggtcgcgca cgcgcgcgcc gtggccacgc ccaaacgctc gtccagtacg gcaaccaggc
     2281 ccgcctctgg tcggcgaacg cgcgcttcgg ctggctcggc acggccggca acgggctcta
     2341 cgtggtgttc aacgagggtc aggcggcgag cggcttcttc cagtgggacc gcccccagac
     2401 gcggtcgctg atcgtgaaat actcacgcca gctgggccgc atcgggtgag gggtcgcggc
     2461 gaccggcaag ccgccgctca gccgacggcg tagttgagca gcacggtccc cgtcttctcg
     2521 tagatccggt gcgacgtcag cctgagcttc gctcgcgtcg ccggactcgg cagcaccggc
     2581 accccaccac ccaggagcac ggggatgatc gcgacctgta cggcgtccac caattcgagc
     2641 gcgagcaggc tctggaacag cccgcccccg ccgaacagcc agatgtcctt cccctgccgc
     2701 tgcttcagcg ccgtgaccgt ttccttcggg tcggccgaaa ccgtcacgcg agggcactct
     2761 tcctggcgca gggtgcgcga gaagacgtag ctcttcatcc cgcgcattgc cggaccgccg
     2821 ccctgctcgc gcgccatctc gtaggtcttt ctgcccatca acagcgtgtc gaagttcttg
     2881 aacagcgctc cgaaatcgat gtccgggtcc atgacgatcc agtcgtaacc gccagtcgga
     2941 tcggcgatgt atccgtcgag actcatcgct actgagtagc gcacgggtcg catggtcggc
     3001 tcttgcaggt gttccctaat tctggcaggc agggggcgaa ttgtcatcat agacgaagcg
     3061 agggaacctt ggcctccatg cgcatcggca tcgttctgga agtgcgtggg gaaagccagg
     3121 ttcggctagc cgcagggctg caaatcgcag cggggtgtga ctcgttccag gccaccaggg
     3181 tcaaaccgct gtatgcgcct ccctgcctct tcagcggtcc gcgccctctc ggctctggtg
     3241 gcgctggcgt tgcccacagc gaggcacgcg tcggcccagg gctcgtggca catgagcctc
     3301 ggcccggaag actcggtcga tatcggcatg ctgcgcaacg cgccgaacgg ccacatctcc
     3361 taccagcaag ccaaaggcgg cctgacgatc tgggtggacg gccggatcgg cgacgcgacc
     3421 caggggacgt tcatactgca cccgcatagc tggagcatcc tggagctcga gcgcgccgcg
     3481 cccgtgcggg tcttcgaggc ggtccacgat tccacgctcc cctgcacgtc cgactccggg
     3541 tggtaccgca actacgccgc gatcaacgcg a
//
LOCUS       DBNGZW010000533         6110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_802602, whole genome shotgun sequence.
ACCESSION   DBNGZW010000533 DBNGZW010000000
VERSION     DBNGZW010000533.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_802602"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..833
                     /locus_tag="AC16JC_12675"
     CDS             <1..833
                     /locus_tag="AC16JC_12675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5165596.1"
                     /translation="LGPNGAGKTTTLGILTTRVKATSGAAFVAGVDVVTDDVAVKQRI
                     GVVPQRPNPDRSLTVFENLVFHAAYFGIPRPDAARKALALLESIGIADRRGAKVDHLS
                     GGQQQRLMIARALIHDPEILFLDEPTVGLDPQARLDLWDLLRGMHREGRTIVMTTHYM
                     EEADRLCERVAIVDRGRLLACDTPRALKGRAPGGTLIELTLEGDAAQAVEQARGHPGV
                     LQAEAQGSILSVYAERPGEVLAQLIRRLEGSGRIVHDIHLAPPSLETLFISLTGRKLA
                     "
     gene            830..1615
                     /locus_tag="AC16JC_12680"
     CDS             830..1615
                     /locus_tag="AC16JC_12680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5165597.1"
                     /translation="MRAPVPATRVFLALLARDLRVARREIVSFLVRTTLQPLLFVVVF
                     GYLLPRMGFVQAGYGAALLPGILGISLALSSLQAVALPMMADFGWTREIEDRLLAPVP
                     IRLIAAEKVVAGVIQGFVAALFVLPVARLIMGPIAALSFAHAGTLLGITLLGSLAFST
                     LGLWMGTGIPPQQIGLMFSMIVAPMIFFGCAYYPWQGLTPLPVVKYAVLVNPLVFVAE
                     GMRGALTPGLPHMPWPLIVLALLVMSAVLWTVGYRSFARRAVS"
     gene            1641..2396
                     /locus_tag="AC16JC_12685"
     CDS             1641..2396
                     /locus_tag="AC16JC_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020551023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HIR5165598.1"
                     /translation="MILEACVDSVESAIAAQTGGAHRIELCEDLLEGGTTPSAGMIEV
                     CRERLHIPVHVLIRPRGGDFVYSDVEIEVMQRDIALARRLGAQGVVFGALQPDGSVDV
                     VRTRALLGVSRPLSVTFHRAFDFTPDADAALDALIGLGIDRVITSGQAPLAVEGIGTL
                     ARLVTRAAGRIRILAAGGLSEENIARVAELTGVREVHVRATALLESPASYRPRHLSLL
                     KQPLPNEFDRAVTDPERIRRISGILEKVAQSRS"
     gene            2396..3976
                     /locus_tag="AC16JC_12690"
     CDS             2396..3976
                     /locus_tag="AC16JC_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007570966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR5165599.1"
                     /translation="MERASLPWRTIPDLLSARAASHGAGDLLRFEGRRMTVAGIHDRS
                     ARLAGVLRTMGVRRGDRVVLLLPNGIEFPVAWLAAVQAGAIVVPVSVRLREADRLHIV
                     RDSGAALAVASPEHAPALSQDRGGCPALRDVLVFGPPGAPEAEPLERVLESAPPLDID
                     PARPDEVVTIQYTSGTTGLPKGCMLTHEYWLELGTRMWHAAEWTSDDIALTAQPFSYI
                     DPVWNLMLCLLAGMPLVVLPRFSASTFWRTVRDQHVTFFYCLGAMPTLLLKQPEDPAV
                     DRGHRVRFVLCSAIPPRVHEAIEDRWGCPWRETYGTTELGVVTLAPLDDRAGVGTGGL
                     GPVVPGKEIRIADGGGADVPAGSVGELLVRGRGLMSGYWNQPEATAEWRRGGWAHTGD
                     LVRREAGRLYLVGRLKDMIRRGGENVSAIEVEAVLCEHPGVRAAACVPVPDELRGEEV
                     KAFVQLAAGETPATLPPPVLLAHVRARLADFKVPRYVEYVDHLPLTPSERVAKHELLA
                     RKADQRRGAWDATAGAWG"
     gene            3986..4120
                     /locus_tag="AC16JC_12695"
     CDS             3986..4120
                     /locus_tag="AC16JC_12695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165600.1"
                     /translation="MALRRTDLEFPGMLALEVDGAVEMLGSSEARELLRDFLTPRPPA
                     "
     gene            complement(4159..5136)
                     /locus_tag="AC16JC_12700"
     CDS             complement(4159..5136)
                     /locus_tag="AC16JC_12700"
                     /inference="COORDINATES: protein motif:HMM:NF016552.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165601.1"
                     /translation="MKLRVTLAVMGGALLAVACSDSSSLITQPAAPSFLDVANSPGQI
                     HVMPTRAVAAGLDNRAKPGGSGGGTGIFYHGGPILASTKVAAIYWGTSRIYNGGPTPG
                     TTGTGGTDGSIVGHFLRNLGGSPYFNINTTYYDGGSVHVSNSVAYAAFWADNSNPGST
                     PSDAAIQAEVVKAFSNGLPYDASTVYLVFTGSGVNLGGGFGSQYCAYHGHFTWSGQDV
                     KYAAQPYNYDFPSGCSALSGSPNNDFAADAEVNTLAHEIEEAATDPDLNAWYDRRGYE
                     NADKCAWTFGSTYKSANGATANMNLGGKDFLIQRNWVNAGSGGCALSYP"
     gene            5276..>6110
                     /locus_tag="AC16JC_12705"
     CDS             5276..>6110
                     /locus_tag="AC16JC_12705"
                     /inference="COORDINATES: protein motif:HMM:NF013300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HIR5165602.1"
                     /translation="MPTRREAIAQLLAAGLAVPGWRAWTGRRMVGDPRLAVPTPAQQA
                     WQDLEVGMFVHFAPNTWQEKEYDDLSSAPADIMPAVDTAQWAETALALGARYIVLVAK
                     HTGGFCLWQTETTDYGIRGTPWRGGHGDVMADLASVCAQRGLRLGVYLSPRDDRHGAG
                     LSGRCATPEAQAAYDALYRRQLTELLTRYGPITELWLDGSSVVPTGDLVARHAPDAMV
                     FQGPQATIRWVGNEDGFAPYPCWNSIAAPDARTGIATALHGDPSGDVWMPVEVDVSIR
                     RP"
BASE COUNT          833 a         2112 c         2200 g          965 t
ORIGIN      
        1 tgctgggacc gaacggcgcc ggcaagacca ccaccctcgg gattctcacg acgcgggtga
       61 aggccacgtc cggcgccgcc ttcgtggcgg gggtggacgt ggtcaccgac gacgtggcgg
      121 tgaagcagcg catcggcgtc gtgccgcaac ggcccaaccc ggaccgcagc ctcaccgtgt
      181 tcgagaatct cgtgttccat gccgcctact tcgggatccc ccgccccgat gcggcgcgca
      241 aagcgctcgc gctgctcgag tcgatcggca tcgccgaccg gcggggcgcg aaagtggacc
      301 acctgtcggg cggccagcag caacgcctga tgatcgcgcg cgcattgatc cacgatccgg
      361 aaatcctgtt cctcgacgag cccacggtcg gcctcgaccc gcaggctcgc ctcgacctct
      421 gggacctgtt gcgcggcatg caccgcgagg gtcgcaccat cgtgatgacc acccactaca
      481 tggaagaggc cgaccggttg tgcgagcgcg tggcgatcgt ggaccggggt cgcctcctcg
      541 cctgcgacac gccccgcgcg ctcaagggcc gtgcgcccgg tggcacgttg atcgagctga
      601 cgctggaagg ggacgcggcc caggcggtcg aacaggcccg tggccatccg ggcgtcctcc
      661 aggcggaagc acagggcagt atcctctcgg tgtacgccga gcgtcccggc gaggtgctcg
      721 cgcagttgat ccgccggctc gaggggtccg gccgcatcgt gcacgacatc cacctcgccc
      781 caccgagtct cgagacgctg ttcatctccc tgaccgggag gaagctcgca tgagagcgcc
      841 ggttcctgcc acgcgggtct tcctggcgct gctggcccgg gacctgcgcg tggcgcgccg
      901 cgagatcgtc tcgttcctgg tccgcaccac gctgcagccg ctgctgttcg tggtggtgtt
      961 cgggtatctc ctgccccgga tgggattcgt gcaggccggc tacggtgcgg cgctgcttcc
     1021 gggcatcctc ggcatctcgc tggcgctctc cagtctccag gccgtcgcgc tgccgatgat
     1081 ggccgacttc ggctggacca gggagatcga ggatcggctg ctggcgccgg tgccgatccg
     1141 gctcatcgcg gcggagaagg tggtggcggg cgtgatccag gggttcgtcg cggcgctgtt
     1201 cgtgctgccg gtggcgcggc tcatcatggg gccgatcgcg gcgctcagct tcgcccatgc
     1261 gggcacgctg ctcgggatca cgctgctcgg ctccctggcg ttctccacgc tgggcctgtg
     1321 gatgggaacg ggaatcccgc cgcagcagat cgggctcatg ttcagcatga tagtggcccc
     1381 gatgatcttc ttcggctgcg cctactaccc gtggcaggga ctcacgccgc tgccggtggt
     1441 caagtacgct gtcctcgtca atccgctcgt gttcgtggcc gaagggatgc gcggcgcgct
     1501 cacccccggg ctgccgcaca tgccgtggcc gctcatcgtg ctcgcgctgc tcgtcatgtc
     1561 ggcggtgctc tggaccgtgg gctaccggtc gttcgcgcgc cgggctgtga gctgagcggg
     1621 aaggcgctat cttggcgcgc gtgatcctcg aagcgtgcgt cgactcggtg gaatccgcga
     1681 tcgccgccca gacgggtggc gcgcaccgca tcgagctgtg cgaggacctg ctggagggcg
     1741 ggaccacgcc cagcgccggc atgatcgagg tctgccggga gcggctgcac attcccgtgc
     1801 acgtgctgat ccgcccgcgg ggtggagact tcgtctactc cgacgtcgaa atcgaagtga
     1861 tgcagcggga catcgcgctc gcgcgccggc tcggcgccca gggcgtggtg ttcggcgcgc
     1921 tgcagcccga cggcagcgtg gacgtggtgc gcacgcgcgc cctcctgggt gtctcccggc
     1981 cgttgtcggt cacgtttcac cgggcattcg atttcacccc cgacgcggac gccgcgctcg
     2041 acgcgctcat cggcctcggc atcgatcgcg tcatcacctc ggggcaggcg cccctggcgg
     2101 tggagggcat cggcactctc gcccgtctcg tgacgcgcgc cgccggccgg atccgcatcc
     2161 tcgcggccgg cgggctgagc gaggagaaca tcgcccgcgt cgccgagctc accggcgtgc
     2221 gggaggtgca cgtgcgcgcg accgcgctgc tcgagagccc cgcctcctac cgcccgcgcc
     2281 acttgtcgct gctgaagcaa ccgctgccca acgaattcga ccgcgccgtg accgatccgg
     2341 agcggatccg gcgcatctcg ggcatcctcg agaaggtcgc ccagtcacgg agctgatgga
     2401 gcgggcgtcg cttccctggc gcacgatccc cgacctcctg agcgcgcgtg ccgcgtcgca
     2461 cggcgccggc gacctgctgc gcttcgaagg ccgtcggatg acggtggccg ggatccacga
     2521 ccggtcggcg cgcctcgccg gcgtgctgcg tacgatgggc gtgcgccgcg gcgaccgcgt
     2581 ggtgctcctg ttgcccaacg gcatcgaatt cccggtggcg tggctggccg cggtgcaggc
     2641 cggggcgatc gtggtccccg tgagcgtcag gctgcgggag gcggaccggc tgcatatcgt
     2701 gcgtgattcc ggcgctgccc tggctgtggc gagtcccgag cacgcgcccg cgctgtccca
     2761 ggaccgcggc ggctgccccg cgctgcgcga cgtgctggta ttcggcccac ccggcgctcc
     2821 ggaggccgag ccgctcgagc gcgtgcttga gtccgcgccg ccgctggaca tcgacccggc
     2881 acggcccgac gaggtcgtca cgatccagta cacctcgggc acgaccggcc tgcccaaggg
     2941 atgcatgctc acccatgaat actggctcga gctcggcacc cgcatgtggc acgccgccga
     3001 gtggacgagc gacgacatcg cgctcaccgc ccagccgttc tcctacatcg acccggtgtg
     3061 gaacctgatg ctgtgcctcc tcgccgggat gccgctcgtg gtcctgccgc gcttctcggc
     3121 gtccacgttc tggcggacgg tgcgggatca gcacgtgacg ttcttctact gtctcggagc
     3181 gatgcccacc ctgctgctga agcagcccga agatccggcc gtggaccgcg gacaccgcgt
     3241 gcggttcgtg ctgtgctcgg caatcccacc ccgggttcac gaggccatcg aggaccggtg
     3301 gggctgcccc tggcgtgaga cgtacggcac gaccgagctc ggcgtcgtca cgctggcgcc
     3361 cctcgacgat cgcgcgggcg tgggcaccgg cggcctgggc cccgtcgtcc ccggcaagga
     3421 gatccggatc gcggatggcg gcggggccga tgtccccgcg ggtagcgtgg gggagctgct
     3481 ggtccgcggc cggggcctca tgtcgggata ctggaaccag ccggaggcga ccgcggaatg
     3541 gcggcgtggc ggctgggccc acacgggcga cctcgtgcgc cgcgaagcgg gtcgtctcta
     3601 tctcgtcggc cggctcaagg acatgattcg ccgcggcggg gagaacgtat cggcgatcga
     3661 agtcgaggcg gtgctgtgcg agcatcccgg cgtgcgggcg gcggcctgcg ttccggtgcc
     3721 cgacgagctg cgcggcgagg aggtcaaggc tttcgtccag ctcgccgcgg gggagacgcc
     3781 cgccacgctg ccgccaccgg tactgctggc ccacgtgcgc gcacggctgg ccgacttcaa
     3841 ggtgccgcgc tacgtcgagt acgtggacca tctgccgctc accccgtcgg agcgggtcgc
     3901 gaagcacgag ctgctggcgc ggaaggccga ccagcgacga ggggcatggg acgcgaccgc
     3961 gggggcgtgg ggttgacccc gcccggtggc gctgcggcgc accgacctcg agtttccggg
     4021 aatgctcgcg ctcgaagtgg acggggcggt cgagatgctc gggagcagtg aggcgaggga
     4081 gctcctgcgg gacttcctga cgccacgacc ccccgcctga cggcgcgggg ggtcgggaaa
     4141 ttcggggcag cgggcccgct agggatagct cagggcgcag ccgccggagc cggcgttcac
     4201 ccaattgcgc tgaatgagaa aatccttgcc acccaggttc atgttggcgg tggcgccgtt
     4261 ggcggacttg tacgtcgagc cgaaggtcca ggcgcacttg tccgcgttct cgtagccgcg
     4321 ccggtcgtac caggcgttga gatcgggatc ggtcgccgct tcctcgatct catgcgccag
     4381 ggtgttcacc tcggcgtcgg cggcgaaatc gttgttgggc gagccgctga gcgcggaaca
     4441 accactgggg aaatcataat tgtacggctg cgccgcgtac ttgacgtcct ggccgctcca
     4501 ggtgaaatgg ccgtggtagg cgcagtactg cgacccgaag ccaccgccca ggttcacccc
     4561 cgagccggtg aagaccaggt acacggtgga ggcgtcgtag ggcagcccgt tcgagaacgc
     4621 cttgaccacc tcggcctgga tggcggcgtc gctcggcgtc gagcccgggt tcgagttgtc
     4681 ggcccagaag gccgcatacg cgacgctgtt gctcacgtgc acgctgccac cgtcgtagta
     4741 ggtcgtgttg atgttgaagt agggcgagcc tcccaggttc cgcaggaaat gccccacgat
     4801 cgagccgtcc gtgccgcccg ttccggtcgt ccccggcgtg ggtccaccgt tatagatcct
     4861 gctcgttccc caatagatcg ccgcgacctt ggtggaggcg agaatcggcc cgccgtgata
     4921 gaagatcccc gtgcccccgc ccgagccgcc cggcttggcg cggttgtcga ggccggccgc
     4981 gacggcccgg gtcggcatca cgtggatctg cccggggctg ttcgccacgt cgaggaacga
     5041 cggagcggcc ggctgggtga tcagactgct ggaatcgctg cacgcgacgg cgagcagcgc
     5101 gccgcccata acggccaggg tgacgcggag cttcatgcgc tagtcctcca ctggtgaggg
     5161 ggaaaggtcg cggccaatct gggggtgaac gcccgcccgg caatggggag ggacgtgaca
     5221 gatcggcggc gcgtggctac catgcctcgg acctcgccgc caggtagcag gatacatgcc
     5281 cacacgcaga gaggcgattg cgcagctgct cgcggccggc ctggccgtgc ccgggtggcg
     5341 tgcctggacc ggccggcgga tggtcgggga tccccggctc gccgtgccga ctccggccca
     5401 gcaggcctgg caggacctcg aggtcgggat gttcgtccat ttcgctccca acacctggca
     5461 ggaaaaggag tacgacgacc tctccagcgc cccggctgac atcatgccgg ccgtggacac
     5521 ggcgcagtgg gccgagaccg cgctcgccct cggcgcccgc tacatcgtgc tcgtcgccaa
     5581 gcacacgggc gggttctgcc tgtggcagac ggagaccacc gattacggca tccgcgggac
     5641 tccgtggcgc gggggccacg gtgacgtcat ggcggatctg gcgtccgtgt gcgcccagcg
     5701 tgggctcagg ctcggggtct acctctcgcc gcgggacgac cggcacgggg cggggttgtc
     5761 gggccgctgc gctaccccgg aggcccaggc cgcctacgac gcgctgtacc gccgccagct
     5821 gacggagctg ctgacccggt acggcccgat cacggagctg tggctcgacg gctcgagcgt
     5881 ggtgccgacg ggagacctgg tggcccgcca cgcgccggac gccatggtct tccaggggcc
     5941 gcaggctacc atccgctggg tcgggaacga agacggattc gcgccctacc cgtgttggaa
     6001 ctccatcgca gcgcccgacg cgcgcaccgg gatcgccacg gcgctgcacg gcgatccctc
     6061 cggggacgtg tggatgcccg tcgaggtcga cgtctcgatt cgccgcccca
//
LOCUS       DBNGZW010000534         4120 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_446765, whole genome shotgun sequence.
ACCESSION   DBNGZW010000534 DBNGZW010000000
VERSION     DBNGZW010000534.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_446765"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="AC16JC_12710"
     CDS             <1..418
                     /locus_tag="AC16JC_12710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR5165603.1"
                     /translation="IIRAGARFVEAMATATVPKLVLTVNHASGAGYYAMAGQGFDPDF
                     ILSWPTGRMGVMEGESAVQAVFGNGVARPENAAAVETMRADYDAQLDARHAGARGHVD
                     AIVLPEDTRDTLVFLLEVTAEFPGPHLGAFVLPPLP"
     gene            415..3261
                     /locus_tag="AC16JC_12715"
     CDS             415..3261
                     /locus_tag="AC16JC_12715"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR5165604.1"
                     /translation="MMSHRLLLLVSLGAACARTPAPGGTAPRPVPTPPPTSVPAAPVA
                     QPDATPVSPPRVADPQLPPPLALLAGLMPLASTGVDAFRGAHPTYDGRGVLIGILDSG
                     IDPGVAGLIVTTTGAPKVLDLRDFSGEGHVALTPVSPGAGGTLAIGGRTLRGAARVGR
                     LAVGSSWYAGLFRELPLGPLPASDVNGNGTNTDAFPVVVVKATDGWVVFFDSNLNGSF
                     EDEQPLHDYRQGRETIALGRQPLTLAANFGEADGVPDLDLYFDTSGHGTHVAGIAAGH
                     WMFGVAGFDGVAPGAQLLGLKIANDARGAISTTGSMRRAMEYAARFASARNLALVLNL
                     SFDVGNERAGRAAIDSLVNDFLHTHPGVVMTISAGNDGPGVSTVEFPGSADLALSVGA
                     LEPGSFTRPPQAGPPPADRMGWWSSRGGATAKPDVVAPGQAFSTVPRWNTGDEIKAGT
                     SMAAPQMAGFAACLLSAMAQERRPVSAADILQAVRATAAPLAGWTDLDQGAGIPKLEA
                     AYRWLVAGHQGSRYVVRTADGSPAALRRDGFAGPADTLQTFAVYHADGLRAAQFQLSS
                     DAAWLTVAPIVTAQARATMIPVRYRPGLLTAPGLYVATVTARSPTDILSGPLFTLTST
                     VVVPVDLAAQALVDSGRALAPGRTQRYFLRSAVPGRTLRVTASVADSDDAVFLQLYDP
                     ASHPASGDPDSLVILGNGKTSTVTIEIPADDMSAGVYELDLLNPGSARATVSLRAQLA
                     PVALAPTPRGDGLEAFNPGAASVSVTTAVALAGAERRWEVAGRGAPAETVTVAVPGWA
                     ARAEVQVSMAVAQWEQFTDFGVTVFDSTGQQVHAAALNYARGRQAFDVPGTIAGHTSR
                     IELFPGFARPDTAPPWQASVRVRFFADAPQSLGPSHLLNVVAGGRTVLPPLGAALPPV
                     ALPEGFAPLLVWRLTSPAGGGRGAAAVAFETVQP"
     gene            3258..>4120
                     /locus_tag="AC16JC_12720"
     CDS             3258..>4120
                     /locus_tag="AC16JC_12720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HIR5165605.1"
                     /translation="MTGQRSVRIAGGQGFWGDWLEAPWRQVTGGPVDYLMMDYLAEVT
                     MSIMQKQKSRDGSLGYAKDFVPLMERLLPVLAERKVRVTTNAGGVNPRACAAAVAEVA
                     RRLGLAGKLTIGVVTGDDLLGRVDTLLAAGHELRNLDTGRPFRDVRDRVQSVNAYLGA
                     GPVVEALRRGADIVITGRVTDTGLTLGPMMHEFGWAPDAWDLIAAGTVAGHTIECGAQ
                     CSGGNCLVDWQRIPDLANVGYPIAEAYADGTVDIIKHPGTGGRITVAGVTEQLVYEMG
                     DPRSYITPDGIA"
BASE COUNT          537 a         1457 c         1509 g          617 t
ORIGIN      
        1 gatcatccgc gccggcgccc ggttcgtcga ggcgatggcc accgcgacgg tccccaaact
       61 ggtgctcacc gtcaaccacg cctccggcgc cggatactac gccatggcgg gacaggggtt
      121 tgaccccgac ttcatcctgt cgtggcccac cggccggatg ggcgtgatgg aaggggagtc
      181 cgcggtgcag gccgtgttcg gcaacggcgt cgcccgcccc gagaacgccg cggccgtcga
      241 gacgatgcgt gccgattacg acgcgcagct cgacgcccgg catgccggcg cgcgcggcca
      301 cgtggatgcc atcgtgctgc cggaggacac gcgcgacacg ctcgtctttc tcctcgaggt
      361 gaccgcggag ttcccaggcc cgcacctcgg cgcgttcgtt cttccacccc tgccatgatg
      421 tcccatcggc ttctgctgct cgtttctctg ggcgccgcct gtgcccggac gcccgcgccg
      481 ggcggaaccg cgccccggcc ggtccccact ccaccgccga catcggttcc cgcggcgccg
      541 gtcgcccagc ccgatgcgac gccggtgtca ccgccgcgcg tggcggaccc ccagctgccg
      601 cctcccctcg ccctgctcgc cggcctgatg ccgctcgcga gcaccggcgt cgacgcgttt
      661 cgtggcgcgc atccgaccta cgacgggcgc ggcgtgctca tcggcatcct ggattccggc
      721 atcgacccgg gcgtggccgg gctgatcgtg acgaccaccg gagcacccaa ggtgctcgac
      781 ctgcgcgatt tctcgggtga gggccacgtt gcgctgactc ccgtgagccc cggtgcgggc
      841 ggcactctcg cgatcggagg tcgcacgctg cgcggcgcgg cgcgggtcgg gcggctcgcg
      901 gtcggctcga gctggtacgc cgggctgttt cgcgagctgc cgctcgggcc gctgccggcg
      961 tcggacgtga acggcaacgg aaccaacacc gacgcgtttc ccgtcgtggt cgtgaaggcg
     1021 acggacggat gggtcgtgtt cttcgattcg aacctgaacg gctcgttcga agacgagcag
     1081 ccgcttcacg actaccgcca ggggcgggag accatcgcgc tgggccggca gccgctgact
     1141 ctggccgcca acttcggcga ggcggacggc gtccccgacc tcgacctgta cttcgacacg
     1201 tccggtcacg gcacccacgt cgcgggcatc gcggcgggac actggatgtt cggggtggcg
     1261 gggttcgacg gcgtcgcgcc cggcgcgcag ctgctgggcc tcaagatcgc caacgacgcg
     1321 cggggcgcga tctcgacgac cgggagcatg cgccgggcga tggagtacgc cgcccggttc
     1381 gcgtccgccc gcaatctcgc cctggtgctc aatctcagtt tcgacgtggg gaacgagcgg
     1441 gcgggacggg cggcgatcga ttcgctcgtc aacgacttcc tgcacacgca ccccggcgtc
     1501 gtcatgacga tcagcgccgg aaacgacggg cccggcgtct ccacggtcga gtttcccgga
     1561 tcggccgatc tcgcgctctc ggtcggcgcg ctggagcccg ggtcgttcac gcgtccgccg
     1621 caggcggggc cgccgccggc cgaccgcatg ggctggtgga gctcgcgcgg cggtgccacg
     1681 gccaagccgg acgtcgtggc tcccgggcag gccttttcca cggtgccgcg ctggaatacg
     1741 ggtgacgaga tcaaggcggg gacgagcatg gcggcgccgc agatggcggg gttcgcggcg
     1801 tgcctcctgt cggcgatggc gcaggagcgg cggcccgtgt ccgccgccga catcctgcaa
     1861 gccgtgcggg cgacggcggc gccgctcgcc ggctggaccg atctggatca gggcgcgggc
     1921 atccccaagc tcgaagcagc ctatcgctgg ctggtggcgg gccatcaggg atcgcgctat
     1981 gtggtccgaa ccgcggacgg aagcccggcg gcgctgcgac gcgacgggtt cgccggtccg
     2041 gccgatacgc tgcagacgtt cgccgtgtac cacgccgacg gactgcgcgc cgcgcaattc
     2101 caactgtcga gtgacgcggc gtggctcacc gtcgccccca tcgtgacggc gcaggcgcgc
     2161 gccaccatga tcccggtccg gtatcgtcca gggctgctga ctgcgccggg actctacgtg
     2221 gcgactgtca cggcccgctc tcccaccgac atcctgtccg gtccgttgtt caccctcacg
     2281 agtacggtgg tcgttcccgt cgatctcgcc gcgcaggcgc tggtggattc gggacgggcg
     2341 ctcgcccccg gccgcaccca gcgctacttc ctgcgctcag ccgttccggg caggacgctg
     2401 cgggtcacgg cgtcggtggc ggactcggac gatgcggtgt tcctccagct ctacgatccg
     2461 gccagccacc cggccagcgg agaccccgac agtctcgtca tcctgggtaa tggaaagacg
     2521 tccacggtca cgatcgagat ccctgccgac gatatgtcgg ccggggtgta cgagctggac
     2581 ctgctcaatc ccgggtccgc gcgggcgacc gtttccctcc gggcgcagct ggccccggtt
     2641 gcgctcgcgc ccacgccccg cggcgacgga ctggaggcgt tcaatccggg cgccgcgtcg
     2701 gtcagcgtca cgaccgcagt ggcactcgcc ggcgcggagc ggcggtggga agtcgccggg
     2761 cgcggtgcgc cggcggagac cgtgacggtg gccgtccccg gctgggcggc gcgagccgaa
     2821 gtgcaggtgt cgatggcggt ggctcagtgg gagcagttca ccgacttcgg cgtgaccgtg
     2881 ttcgactcga cgggccagca ggtgcatgcg gccgcgctca actatgcgcg cggccgccag
     2941 gcgttcgacg tgccgggcac gatcgccggc cacacttcgc ggatcgagtt gtttccggga
     3001 ttcgcgcggc cggacacggc gccgccctgg caggcgagcg tccgggtgcg gttcttcgcc
     3061 gatgcgccgc agtcgctcgg gccgtcgcat ctcctgaacg tcgtcgccgg cggccggacc
     3121 gtgcttcccc cgttgggggc ggcgctgccc cctgttgcgt tgccggaagg gttcgcgccg
     3181 ctgctcgtgt ggcgcctcac ctcgcccgcc ggagggggga gaggggcggc ggcggtcgcg
     3241 ttcgagacgg tgcagccatg acggggcagc ggtcggtgcg catcgcgggc ggtcagggct
     3301 tctggggcga ctggctcgag gcgccctggc ggcaggtgac cggcggcccc gtggactacc
     3361 tgatgatgga ctatctcgcg gaagtgacga tgtccatcat gcagaagcag aagtcgcgcg
     3421 acggatcgct cggctacgcc aaggacttcg tgccgctcat ggaacgactc ctgccggtcc
     3481 tcgcggagcg gaaggtgcgc gtgacgacga acgccggggg cgtgaacccc agggcctgcg
     3541 ccgcggcggt ggccgaggtg gcccggcggc tcggcctcgc cggcaagctg acgatcggcg
     3601 tggtgacggg ggacgatctg ctggggcggg tggacacgct cctcgcggcg gggcacgagc
     3661 tgcggaacct cgacaccggc cggccgttcc gcgatgtgcg cgaccgcgtg cagtcggtca
     3721 acgcgtacct cggcgccggt ccggtcgtcg aggcgctgcg ccgcggcgcg gacatcgtga
     3781 tcaccggccg ggtcaccgac acgggactca cgctgggccc gatgatgcac gaattcggct
     3841 gggcgccgga cgcctgggac ctcatcgcgg cagggaccgt cgccggccac acgatcgaat
     3901 gcggcgccca gtgctcgggc ggcaactgtc tcgtggactg gcagcgcatt cccgacctgg
     3961 cgaacgtggg ctacccgatc gcggaagcct acgccgacgg gacggtggac atcatcaagc
     4021 atcccggcac gggcgggcgt attacggtcg cgggcgtcac cgagcagctc gtgtacgaga
     4081 tgggtgaccc acggagctac atcacgcccg acggcatcgc
//
LOCUS       DBNGZW010000535         1870 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_936020, whole genome shotgun sequence.
ACCESSION   DBNGZW010000535 DBNGZW010000000
VERSION     DBNGZW010000535.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1870)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1870)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1870
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_936020"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="AC16JC_12725"
     CDS             complement(<1..1485)
                     /locus_tag="AC16JC_12725"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR5165606.1"
                     /translation="MPVDTVRDTGQRRWQNWHQTVDQSLERWLVVSNADPHQSTIAGY
                     NATTRVLQDLMGQARDKDLSIRAHGGTWSFSPIAATEGILLNTQPLNYRFRMGAEQFH
                     PANAGGAALIFAQCGMAIVELNRYLAPLGLALQTCGASNGQTIAGAISTGTHGAALRF
                     GAMQDYVRALHLIVSPEESVWLEPASRPAVRDELAGFVNARLIRDDELFYAALVSFGS
                     FGLIHGVLLEAAPMYYLQSWRETRALDDTLWRAIASLDFSALTLQGAAGRQPDHFQVL
                     INPFDQSRQGIVTAMYRVPSRPPGSTPPKTGNEWTQGDSAFEAVGLITDLLPDASALL
                     ASLIDKLTRTTVANVCGEPGQMFRDTTTRGKTAGAAIGVPLDRTRDAIDVARNEIGRT
                     RAPALLAIRLVRSTKATLGFTSFEPATAVIDIDCPQSNRQRDLLHGLWPALEQAGIPF
                     RFHWGKLHETDRDRLRRMYGDRVDRWLSARRTLLPTPELRRLFAN"
     gene            complement(1488..>1870)
                     /locus_tag="AC16JC_12730"
     CDS             complement(1488..>1870)
                     /locus_tag="AC16JC_12730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165607.1"
                     /translation="QQLAHGLMIATLALFSFPAGGCLAYRANALGAAPIEEPMRVEGD
                     TALVVREQVTIWSLAYGLVRKGRSRIECDGQGLQEVNLTSPPHYTLLTIITLGAVSPK
                     RLRTKCAKAIPAGGVFAPPPSGAQ"
BASE COUNT          269 a          621 c          688 g          292 t
ORIGIN      
        1 gttggcgaac aggcggcgaa gctcgggggt gggaagcagc gtccgccgcg cggacagcca
       61 ccggtcgacg cggtccccgt acatcctgcg cagccggtca cggtcggtct cgtggagctt
      121 gccccaatga aaccggaacg ggattcccgc ctgctcgagg gccggccaga ggccgtgcag
      181 caggtcccgc tgacggttcg attgcgggca gtcgatgtcg atcacggccg tggccggctc
      241 gaagctggtg aatcccagcg tcgccttggt ggacctcacg agccggatgg cgagcagcgc
      301 cggcgcgcgg gttcgtccaa tctcgttgcg ggccacgtcg atggcgtccc gggtgcggtc
      361 cagcggcacg ccgatggcag ccccggcggt cttgccccgc gtggtcgtgt cgcggaacat
      421 ctgccccggc tcgccgcaca cgttcgcgac ggtggtccgc gtcagcttgt cgatgagcga
      481 cgcgagcagc gcgctggcat ccggcaggag gtcggtgatg agcccgacgg cctcgaacgc
      541 ggagtcgccc tgggtccact cgttgccggt cttcggcggc gtgctgcccg ggggccggct
      601 cggcacgcga tacatggcgg tgacgatgcc ctgcctgctc tgatcgaagg gattgatgag
      661 cacctggaag tggtccggtt gccggccggc cgctccctgg agcgtcagcg ccgaaaagtc
      721 gagcgacgcg atggcccgcc agagcgtgtc atccagggcg cgcgtctcgc gccagctctg
      781 gaggtagtac atcggcgccg cttcgagcag caccccgtga atgaggccga aactgccgaa
      841 gctcaccagc gcggcgtaga acagctcgtc gtcccggatg agccgggcgt tgacgaagcc
      901 ggccagctcg tcgcggaccg ccggccgcga ggccggctcg agccagacgg actcctcggg
      961 cgagacgatc aggtggaggg cccgcacgta gtcctgcatc gcgccgaacc ggagcgcggc
     1021 gccgtgggtg ccggtcgaga tcgcgccggc gatcgtctgc ccgttgctcg cgccgcaggt
     1081 ctgcagcgcc aggccgagcg gggcgaggta gcggttcaac tcgacgatcg ccatcccgca
     1141 ctgcgcgaag atcagggccg ctccgcctgc attggcggga tggaactgct cggcgcccat
     1201 gcggaagcgg tagttgagcg gctgggtatt gagcagaatg ccctcggtcg ccgcgatggg
     1261 cgagaacgac caggtcccgc cgtgcgcccg gatcgacagg tctttgtccc gcgcctggcc
     1321 catcaggtcc tgcagcactc gggtggtcgc gttgtatccc gcaatggtcg actgatgcgg
     1381 gtcggcgttc gagaccacca gccagcgctc gagcgactgg tcgaccgtct ggtgccagtt
     1441 ctgccaccgc cgctgccccg tgtcccgtac cgtgtccacg ggcatgctca ctgtgctcca
     1501 ctgggtgggg gcgcgaagac gccaccggcg gggatcgcct tggcgcactt ggtccggagc
     1561 cgtttgggcg acaccgcccc cagggtgatg atcgtgagga gcgtgtaatg ggggggactg
     1621 gtgaggttca cctcctggag gccctgcccg tcgcactcga tgcggctgcg ccccttccgg
     1681 acgagcccgt aggccaggga ccagatggtg acctgctcgc ggacgacgag cgcggtgtct
     1741 ccttcgaccc gcatcggctc ctcgatcgga gccgcgccga gggcgttggc gcggtacgcc
     1801 aggcagccgc ctgccgggaa ggagaagagc gcgagcgtgg cgatcatcag gccatgcgcg
     1861 agctgctgtt
//
LOCUS       DBNGZW010000536         7656 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_998208, whole genome shotgun sequence.
ACCESSION   DBNGZW010000536 DBNGZW010000000
VERSION     DBNGZW010000536.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7656)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7656)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7656
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_998208"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="AC16JC_12735"
     CDS             <1..333
                     /locus_tag="AC16JC_12735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012962804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5165608.1"
                     /translation="EAGFPIVAPPPGSDVYLLAQMWKWTPILKLQKGVEYTLHLSALD
                     VNHGFNLNPVNINFQVVPGYDYGLKIVPNAAGDFRIVCDEFCGIGHHTMVGKVIIVDS
                     AAAPGGTP"
     gene            330..2033
                     /locus_tag="AC16JC_12740"
     CDS             330..2033
                     /locus_tag="AC16JC_12740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR5165609.1"
                     /translation="MTAATAPADFRTCPRTNLKIHAAADALIKANAVAATVALLIGGV
                     AAILVLLTRWQAVHLLPADWFYRILTIHGMSMLIFFIIFFEMAILYFASAVVLNSRVA
                     APRLGWVAFVLMVLGAVMTEATMWTGHADVLFTSYIPLQATSWFYLGIILFAVGALVV
                     VFIFFGTLVVAKRERTYQGSVPLVTFGALTAAIIAFITLAHGAAIYIPTYLWSLGLMK
                     VDPEIYRMVWWGLGHASQQINVAAMVSVWYLLGALTVGSIVLNEKVSRGAFALYILFI
                     SMASAHHLLVDPGLGSAWKVWNTSYFMYMAVLASMVHGFTVPAGLEVGQRARGLTKGM
                     FEWLRKAPWGDPGFSAMIFSIVVFGFVGGITGVTFGTEQINIIAHNTLRIPGHFHATV
                     VSGTALAFMGLTYYVIPLIFRRRIAFYPLAKIQPWLFAIGMLIFSLMMTFAGTFGVPR
                     RHWDITFSGAPFDVQFNPAVNLVIGLMALGGLLAAVGGGIYILVTVWSVFFGERLPAG
                     PLTGHGLPPGIATPPKPIAPGDHPKKATGTLVLVFVFLAAFIAYYFINWKLLSVVWKI
                     G"
     gene            2018..2941
                     /locus_tag="AC16JC_12745"
     CDS             2018..2941
                     /locus_tag="AC16JC_12745"
                     /inference="COORDINATES: protein motif:HMM:NF014667.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HIR5165610.1"
                     /translation="MEDRLNAAPIPERLAPRHWGTLVALGAILLITVAWWGLALWPLP
                     AEAPPALLRARTVCFGTGDNGLPNGIGWMMLIGEPIMITLLLVSAVGGMTVRETFQGL
                     ARHTLGRLALNTTVLGLVAALALAGARVTYALGIGAADPAQSNTDVAVSRIGRPAPPL
                     DLVDQHGTTVTLAALRGRPTLVTFAYAHCQTVCPLIVLNVVRARNQLAGQGAAPQIVV
                     ITLDPWRDTPSRLPDMARQWGLPDDAHVLSGSVQDVEAALHRWEIPRTRNGQNGEITH
                     PNVVYVLDGSGKIAFVANGDADVLAQLVNRL"
     gene            complement(2992..3408)
                     /locus_tag="AC16JC_12750"
     CDS             complement(2992..3408)
                     /locus_tag="AC16JC_12750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165611.1"
                     /translation="MPGASVWFIRAALAYLLAGFTLGAWLLAAKGLPLPLGVWRWLPV
                     HYELLLVGWFVQLVMGVAYWIFPRFGMTRAARGREGLAWLALGLLNVGIWSVSVAVLV
                     GPATLAVIGRVMEVLAAATMALNVWARTRASGISPM"
     gene            complement(3413..3811)
                     /locus_tag="AC16JC_12755"
     CDS             complement(3413..3811)
                     /locus_tag="AC16JC_12755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012257828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165612.1"
                     /translation="MPPITRWFVKFSLVYFVAALGGSVWQAAGGPFWLTPVIIHLLVV
                     GWVTGMIFGVAYWMFPKYSAEQPRGVDSLAVASFVLLNLGLLLRIAAEPVQTTHPSPV
                     AGWTLVASATLQWLGALAFVVNTWPRVKGR"
     gene            complement(3815..4204)
                     /locus_tag="AC16JC_12760"
     CDS             complement(3815..4204)
                     /locus_tag="AC16JC_12760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009674045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR5165613.1"
                     /translation="MVQRLNISGNSPYEPVVGYSRAVRVGNLIHVAGTTATDEQGALV
                     GIGDPYLQARQALRNIERALVRAGATLRDVVRTRTYVTDISRWQEVGKAHGEVFGSIL
                     PAATMVQVSGLVDPAMLVEIEADAVVA"
     gene            complement(4238..5158)
                     /locus_tag="AC16JC_12765"
     CDS             complement(4238..5158)
                     /locus_tag="AC16JC_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015744894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HIR5165614.1"
                     /translation="MRVIVSGASGLIGTALVPALRAAGHDVGRLVRGRAATVPGDVTW
                     SPGEGVIDAAGLEGVDGVVHLSGEPIFPGWGRRWTPAKRARIRESRVDTTALLARSLA
                     GLVHRPRVLVLITGSGHYGDRADEVLTEESTGGTGFLAEVSREWEAAARPAAAAGVRV
                     VCLRTGLVLDRRGGLLGPLLLPFRLGLGGPIGRGRRYWSWITIDDLVAVFRFALESSA
                     LAGPVNTATPNPVTNREFTAVLGRVLRRPAFVPVPPFALRLLFGREAANEAMLTSARL
                     VPVRLLAAGFKFRFPEVEGALRHVLRSDSR"
     gene            5248..5628
                     /locus_tag="AC16JC_12770"
     CDS             5248..5628
                     /locus_tag="AC16JC_12770"
                     /inference="COORDINATES: protein motif:HMM:NF012610.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR5165615.1"
                     /translation="MFDHVDPRSPTPLYAQIASRLRIAVAAGELRPGDALPSVRRLAA
                     QLRVNPATVVQAYRDLEAEGFVEMRQGTGTFVRELPPARRSSERVRQATVLVRQLIAN
                     ARRLGVSLDELHSAILEEIGAKTS"
     gene            5625..6533
                     /locus_tag="AC16JC_12775"
     CDS             5625..6533
                     /locus_tag="AC16JC_12775"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5165616.1"
                     /translation="MSHAIEIRRLHYRAGKSFEIPSLDLTVPTGSIYGFLGPNGSGKT
                     TTMRLVLGLLRPRAGEIRVLDRPMPQDAPCVLARIGYVPEQPHLDPTLTVGELLRFQA
                     AFYATWDHAWAAQLLTTFELDGRHPFGRLSKGQKGKLMMLLALAQRPELLVLDEPTDG
                     LDPVVRRDMLAALLDYVSERGATVFISSHLVHELERMCDWIGVMDQGRLVTELPMERF
                     KNGIERLVVADAPLEVAATPFSLLSRARNGATTETWLVRDWEPEMQSYFDTAGASLQR
                     VIDLDLEEGFVELLRTFRTPGRGEAR"
     gene            6533..7189
                     /locus_tag="AC16JC_12780"
     CDS             6533..7189
                     /locus_tag="AC16JC_12780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165617.1"
                     /translation="MFRVLLYSQWKWSRLVVTLGTIAAFVLPILSVQGFARDAASPAR
                     SGEMLSAMQSWGVLYPVLAAALGLLVAMATWAPDHRGRHVHALSLPLPRWRYALLRFA
                     AGLVLLAPPVLGLLVGALLATRMAPIPAGLEGYPIALVIRFALATAVAFSVFFAISAG
                     TTRTAAIILATLGGVIAIQVIAGAANVQIDLLTPLQSAFLNWPGPLAIFTGRWMLIDV
                     "
     gene            7257..>7656
                     /locus_tag="AC16JC_12785"
     CDS             7257..>7656
                     /locus_tag="AC16JC_12785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165618.1"
                     /translation="MFTAPLVSQSTAALQSRADSLLREWRQANVFAVVQDSLHAAQRI
                     AGRDTIHVGALTILANPSPLPLAQAAAQAWPEIERFYGPAAQALARHPVVIQAVDPDT
                     AVLTSGPAGILRIPWDQDQGQLVRTLVGSAD"
BASE COUNT         1173 a         2770 c         2488 g         1225 t
ORIGIN      
        1 gaagcgggct tcccgatcgt cgccccgccg ccgggcagcg acgtatacct cctcgcgcag
       61 atgtggaagt ggacgccgat tctgaagctc cagaaggggg tcgagtacac gctgcatctc
      121 tcggcgctcg acgtgaacca cggcttcaac ctcaatcccg tcaacatcaa tttccaggtc
      181 gtcccgggct acgactacgg cctcaagatc gtccccaacg ccgccgggga tttccgtatc
      241 gtctgtgacg agttctgcgg catcggccac cacacgatgg tcgggaaggt catcatcgtc
      301 gactccgccg cggcgccggg aggcacccca tgaccgccgc caccgccccc gcggacttcc
      361 gtacctgccc gcgcacgaat ctgaagatcc acgccgcggc tgatgcgctc atcaaagcca
      421 acgccgtggc cgccaccgtc gccctgctga tcggcggcgt cgccgcgatc ctcgtcctgc
      481 tgacccgctg gcaggcggtg cacctgctcc ccgcggactg gttctaccgc atcctgacga
      541 tccacgggat gagcatgctg atcttcttca tcatcttctt cgagatggcg atcctgtact
      601 tcgccagcgc ggtcgtgctc aacagccgcg tcgccgctcc acggctgggc tgggtcgcgt
      661 tcgtcctgat ggtgctcggc gcggtgatga ccgaggccac catgtggacc gggcacgcgg
      721 atgtgttgtt cacctcctac atcccgttgc aggctacctc ctggttctac ctgggcatca
      781 ttctgttcgc ggtgggcgcc ctcgtggtcg tgttcatctt cttcggcacg ctcgtggtgg
      841 ccaaacgcga gcgcacctac cagggctcgg tgccgctcgt cacgttcggc gcgctcacgg
      901 cggccatcat cgccttcatc acgctcgccc acggcgcggc gatctacatc cccacctacc
      961 tctggtcgct cgggctcatg aaggtcgatc ctgagatcta ccgcatggtc tggtgggggc
     1021 tggggcatgc ctcgcagcag atcaacgttg ccgcgatggt ctccgtctgg tatctgctcg
     1081 gcgcgctcac cgtcggctcg atcgtcctga acgaaaaggt cagccgcgga gcattcgccc
     1141 tctacatcct cttcatctcg atggcctcgg cgcaccacct gctcgtggac cccggcctgg
     1201 gctccgcctg gaaggtatgg aacacgagct acttcatgta catggccgtg ctcgcctcca
     1261 tggtccatgg attcacggtc ccggcagggc tcgaggtcgg ccaacgggcg cggggcctca
     1321 cgaagggcat gttcgagtgg ctgcgcaagg cgccgtgggg ggatcccggc ttctccgcca
     1381 tgatcttctc gatcgtggtc ttcggcttcg tgggcggcat caccggcgtc accttcggga
     1441 cggagcagat caacatcatc gcccacaaca cgctgcgcat cccgggacac ttccacgcca
     1501 ccgtcgtgag cggcaccgcc ctcgcgttca tgggactcac ctactacgtg atcccgctga
     1561 tcttccggcg gcggatcgcg ttttatccgc tcgccaagat ccagccgtgg ttgttcgcga
     1621 tcgggatgct catcttctcg ctcatgatga cgttcgccgg caccttcggc gtgccgcggc
     1681 gccactggga catcaccttc tccggggcgc cgttcgacgt gcagttcaat ccggcggtga
     1741 acctcgtcat cggcctcatg gcgctggggg ggctcctcgc ggcggtgggt ggcgggatct
     1801 acatcctggt gaccgtgtgg tccgtgttct tcggtgagcg cctcccggct gggccgctca
     1861 cggggcacgg gctgccgccg ggcatcgcga cgccgccgaa acccattgcg ccgggcgatc
     1921 accccaagaa ggcgaccggg acactggtgc tcgtcttcgt cttcctggcg gcgttcatcg
     1981 cctactactt catcaactgg aaactgctct ccgtcgtatg gaagatcggc tgaacgcagc
     2041 acctatcccc gaacggctcg ccccgcgcca ctggggaacc ctggtggcgc tgggcgccat
     2101 cctcctgatc accgtggcct ggtgggggct ggcgctctgg cccctgccgg ccgaggcccc
     2161 gcccgcgctg ctgcgggcgc gcaccgtctg cttcggcacc ggcgacaacg gcctccccaa
     2221 cggcatcgga tggatgatgc tgatcggcga gccgatcatg atcacgctcc tgctggtgag
     2281 cgcggtgggc ggcatgaccg tgcgcgagac gttccagggc ctcgcccggc acacgctggg
     2341 ccggctcgcg ttgaacacga cggtgctcgg cctggtggcg gccctcgccc tcgcgggggc
     2401 gcgggtgacc tacgccctgg ggatcggtgc ggcggacccg gcacaatcca ataccgacgt
     2461 cgccgtcagc cgcatcggcc ggccagcccc gcccctggat ctcgtggatc agcacggcac
     2521 caccgtgacg ctcgccgcgt tgcgcgggcg gccaacgctc gtcacgttcg cctacgccca
     2581 ctgccagacc gtgtgcccgc tcatcgtgct gaatgtcgtg cgggcccgca accagctcgc
     2641 cggtcagggt gcggcgccgc agatcgtcgt gatcacgctc gatccctggc gcgatacgcc
     2701 gtcccgtctc cccgacatgg cccggcaatg gggattgccg gacgatgccc acgtgctgag
     2761 cggatccgtg caggacgtcg aggcggccct gcaccgctgg gagatcccgc gtacgcgcaa
     2821 cggccagaac ggcgagatca cccacccgaa cgtggtgtac gtgctcgacg ggtccggcaa
     2881 gatcgccttc gtcgcgaacg gcgatgccga tgtcctggcc cagctggtga atcggctctg
     2941 aggtctgctg atggctgacg gctgatagcc gatagccgat agccgaacct gctacatcgg
     3001 cgaaatcccc gacgcccgcg tccgcgccca cacgttcagc gccatcgtgg cggccgccag
     3061 gacttccatc acgcgaccga tgacggccag cgtcgccggt ccgaccagca ccgcgacgct
     3121 cacggaccag atcccgacgt tgagcaggcc gagcgcgagc caggcgagcc cttcacggcc
     3181 acgggccgct cgcgtcatgc cgaagcgcgg gaagatccag taggcgaccc ccatcacgag
     3241 ctgcacgaac caccccacca gcaacagctc gtagtgcacc gggagccagc gccacacgcc
     3301 gagcggaagc ggcagtccct tggctgcgag cagccatgcg ccgagcgtga acccggcaag
     3361 cagataggcg agtgcggcgc ggatgaacca gaccgaggcc cctggcatcc cgctaccgcc
     3421 ccttcacccg gggccaggtg ttgacgacga acgccagcgc gccgagccac tgcagcgtgg
     3481 ccgaggcgac gagcgtccag cccgccacgg gcgacggatg cgtggtctgc accggctccg
     3541 ccgcgatccg cagcagcagg cccagattga gcagcacgaa gctcgccacc gcgagactgt
     3601 ccacgccgcg cggttgctcg gcggaatact tggggaacat ccagtaggcg actccgaaga
     3661 tcatcccggt gacccacccg acgacgagca ggtggatgat gacgggcgtg agccagaacg
     3721 gaccgcccgc cgcctgccag acgctgccgc cgagggcggc gacgaagtac accagggaga
     3781 acttgacgaa ccagcgagtg atcggcggca tgggttatgc cacgaccgcg tcggcttcga
     3841 tctcgaccag catcgcggga tccacgaggc cgctcacctg caccatggtc gcggccggga
     3901 gaatcgagcc gaagacctct ccgtgtgctt tccccacttc ctgccaccgc gagatgtcgg
     3961 tcacatatgt gcgagtccgc accacgtccc gcaacgtcgc ccccgcgcgc acgagcgccc
     4021 gctcgatgtt ccgcagcgcc tgccgcgcct ggagataggg atcgccgatg ccgaccagcg
     4081 ccccctgctc gtccgtggcc gtggtcccgg cgacatggat gaggttgccg acccgtacgg
     4141 cccgcgagta cccgacgacg ggctcatagg gcgagttgcc ggaaatgttg agacgctgga
     4201 ccatagaacc tcggggaaag aagctatcgg ctatcagcta tcggctatca gaacgaagaa
     4261 cgtgccgcag cgcaccctcg acctcgggaa agcgaaactt gaatccggca gcgagcaacc
     4321 gcaccggcac gagccgtgcg ctggtcaaca tcgcctcatt cgctgcctcc cggccgaaga
     4381 gcagccggag cgcgaacggg ggtaccggca cgaacgcggg ccggcgcaag acccgaccga
     4441 ggaccgcagt gaactcccgg ttggtcaccg gattgggcgt tgcggtgttg acggggccgg
     4501 ccagagcgga cgactcgagc gcgaagcgga agacggcgac gaggtcgtcg atcgtgatcc
     4561 agctccagta gcgccggccc cgtccgatcg gcccgcccaa ccccaggcgg aacggcagca
     4621 gcagaggccc aagcaggcca ccgcgccggt ccaagacgag accggttcgc aagcacacga
     4681 cccgcacgcc ggccgctgcc gccggacgcg ccgcggcttc ccactcgcgc gacacctccg
     4741 cgaggaaccc ggtgcccccg gtgctctctt ccgtcagtac ttcgtcggct cgatcgccgt
     4801 agtgcccact ccccgtgatg agcacgagga ctcggggcct gtgcaccagc ccggcgagcg
     4861 agcgggccag caacgccgtg gtgtccaccc ggctctcccg gatgcgggca cgcttggccg
     4921 gtgtccagcg acggccccaa ccgggaaaga tcggctcgcc cgagaggtgg accactccgt
     4981 ccaccccttc gagccccgcg gcatcgatga cgccctctcc cggtgaccag gtcacgtcgc
     5041 ctggaacggt ggccgcgcga ccgcgcacca gacgtccgac gtcgtgcccc gcggcgcgga
     5101 gcgccggcac cagggcggtg ccgatcagac cgctcgctcc cgacacgatc acgcgcatgg
     5161 gtcggaggca acctagctgc ctcgatcttg cgcggcacca cacattgtag tactgtatcg
     5221 gtacaatgat acagtcggcc gcgccacgtg tttgaccatg tggacccgcg cagccccacc
     5281 cccctgtacg cccagatcgc gtcgcggctg cgcatcgcgg tggcggcagg ggaactgcgg
     5341 ccgggcgacg cgctgccgtc ggtccgccgg ctcgcggcgc agctgcgcgt gaatccggcg
     5401 acggtggtgc aggcctatcg cgacctcgag gcggaagggt tcgtggagat gcggcagggg
     5461 acaggcacct tcgtgcgcga gctcccgccc gcgcggcgca gcagcgagcg ggtccgtcag
     5521 gcgaccgtcc tggtgcgcca gttgatcgcg aacgcccggc ggctgggcgt gtcgttggac
     5581 gagttgcaca gcgcaattct ggaagagatc ggagcgaaga cctcatgagc cacgcgatcg
     5641 agatccggcg cctccattat cgcgccggca agagcttcga gatcccgagc ctcgacctga
     5701 cggtcccgac gggctcgatc tacggcttcc tggggcccaa tggatcgggg aagaccacga
     5761 cgatgcggct ggtgctcggc ctcttgcggc cgcgcgccgg cgagatccgc gtgctcgacc
     5821 gccccatgcc ccaggacgcg ccgtgcgtgc tggcgcggat cggctacgta ccggagcagc
     5881 cgcatctcga tcccaccctc accgtcggcg agctgctgcg gttccaggcg gcgttctacg
     5941 cgacctggga tcacgcgtgg gcggcgcagc tgctcacgac gttcgagctg gatggccgcc
     6001 accccttcgg ccggctgtcc aaggggcaga agggcaagct catgatgctc ctcgcgctgg
     6061 cgcagcgccc cgagctgctg gtgctggacg agccgaccga cggactcgac cccgtggtgc
     6121 gccgcgacat gctggcggcg ctgctcgatt acgtctccga gcgcggcgcc acggtgttca
     6181 tctccagcca cctggtgcac gagctggagc gcatgtgcga ctggatcggc gtgatggacc
     6241 agggccggct cgtgaccgag ctcccgatgg agcgcttcaa gaacggcatc gaacgcctgg
     6301 tggtggcgga cgcgccgctc gaggtcgccg ccacgccgtt ctcgctgctc tcccgcgccc
     6361 gcaacggcgc gacgaccgag acctggctgg tgcgggactg ggagcccgag atgcagtcgt
     6421 acttcgacac ggcaggagcc tcgctgcagc gggtgatcga tctcgacctg gaggagggct
     6481 tcgtcgagct gctgcgcacg ttccgcacac ccggaagggg ggaggcccgc tgatgtttcg
     6541 cgtgctgctc tattcccagt ggaagtggtc gcgcctggtc gtcaccctcg gcacgatcgc
     6601 ggccttcgtc ctccccattc tgtcggtcca ggggttcgcg cgggacgccg cgagccccgc
     6661 gcgctcagga gaaatgctca gcgccatgca gtcgtggggc gtgctctacc ccgtgctcgc
     6721 agcggcgctg gggctgctcg tcgccatggc cacgtgggcc ccggaccacc gcggccgcca
     6781 cgtccatgcc ctctccctgc ccctgccccg ctggcgctac gcgctgctgc gcttcgcggc
     6841 aggactcgtc ctcctcgcac cgccggtcct gggtctcctg gtgggtgcgc tgctcgcgac
     6901 gcgcatggct ccgatccccg cgggactcga gggttatccg atcgccctcg tcattcgatt
     6961 cgcactcgcg acggccgtcg ccttctccgt cttcttcgcg atctccgccg gcacgacccg
     7021 caccgccgcc atcatcctgg cgacgctcgg cggtgtgatc gcgatccagg tcatcgcggg
     7081 cgccgccaac gtgcagatcg acctgctcac acccttgcag agcgcgttcc tcaactggcc
     7141 cggcccgctg gcgatcttca ccggccgctg gatgctgatc gatgtatagc ggggccgagc
     7201 cgggccttca ggccacgtcg gcccacgcag gccgactcgt tccggtgttt gcactcgtgt
     7261 tcacggcccc tctggtcagc cagagcaccg cggccctgca gtcccgcgcc gacagcctgc
     7321 tgcgcgagtg gcggcaagcc aacgtgttcg cggtcgtgca ggactccctg cacgccgccc
     7381 agcgcatcgc cggtcgcgat acgatccacg tcggcgcgct gacgatcctt gccaacccct
     7441 cgccattacc gctggcgcag gcggcggctc aggcctggcc cgagatcgag cggttctacg
     7501 gccctgcagc ccaggccctc gccaggcatc cggtggtcat ccaggcggtg gatcccgaca
     7561 ccgcagtgct cacctccggc ccggcgggca tcctccggat cccgtgggac caggaccagg
     7621 gccaactcgt ccgcacgctg gtaggctccg cggacc
//
LOCUS       DBNGZW010000537         1889 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1684915, whole genome shotgun sequence.
ACCESSION   DBNGZW010000537 DBNGZW010000000
VERSION     DBNGZW010000537.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1889)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1889)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1889
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1684915"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="AC16JC_12790"
     CDS             <1..265
                     /locus_tag="AC16JC_12790"
                     /inference="COORDINATES: protein motif:HMM:NF016836.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008289 - lipid binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HIR5165619.1"
                     /translation="PLMGVRAVDNQITVRPKWMTGELQTSVTAALRHRHDLHTQHVHV
                     ETVRGVVVLQGYVPSLAERAAIERVAWQAPGVLGVVNELSIER"
     gene            359..1279
                     /locus_tag="AC16JC_12795"
     CDS             359..1279
                     /locus_tag="AC16JC_12795"
                     /inference="COORDINATES: protein motif:HMM:NF013761.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HIR5165620.1"
                     /translation="MSIARSFLLRASRSPWLAEQFRRRAFARRAVRRFMPGESLLDAL
                     DASAELAKASMGTVLTQLGEQVRNAAEAEDVRDHYLNVFDQVRDRFLPAQVSVKLTHL
                     GLDVDRDTCLRHVRALAEKARKEGCFLWIDMEESRYVDATLDLYRRVRQEGPGVGVCL
                     QAYLRRTPQDLAALLPLSPAIRLVKGAYNEPGTVALPRKTDVDAQYSTLAVQLLDAAA
                     AGLATTVFGTHDLPLIERIREQAAARRADGRYEVHMLYGIRSDAQRRLAGQGVKVRVL
                     ISYGSHWFAWYMRRLAERPANVWFVVRSLF"
     gene            complement(1276..>1889)
                     /locus_tag="AC16JC_12800"
     CDS             complement(1276..>1889)
                     /locus_tag="AC16JC_12800"
                     /inference="COORDINATES: protein motif:HMM:NF014794.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5165621.1"
                     /translation="QEAFGPETVFSTDSGNGTFLAMECLRLECPGRFLAPVDYSCMGY
                     AVPAALGAKLARPECPVVALAGDGALLMTGLELLTAAQLGIAVAVFVLRDRELAQIAQ
                     FQATALSRKTASVLPDYDAGALAGALGVEAVTLARDADIATVLARVREILAVGRPVLV
                     DVAIDYSRKTFFTAGVVKTNLLRFSWPERLRFIGRALSRKVLG"
BASE COUNT          285 a          701 c          635 g          268 t
ORIGIN      
        1 gccgctcatg ggcgtccggg ccgtcgacaa tcagatcacc gtgcgcccga agtggatgac
       61 cggcgagctc cagacctccg tgacggccgc gttgcggcac cgccatgacc tgcacacgca
      121 gcacgtgcac gtcgaaacgg tacgcggcgt ggtcgtgctg cagggctatg tcccgtccct
      181 cgccgagcgt gccgcgatcg agcgcgtcgc gtggcaggcg cccggtgtcc tgggcgtggt
      241 gaacgagctg tccatcgagc gctgagcaaa gctggtacgg agcgggtcgc tcgccgggcc
      301 ccgggtcgcc ggggcccggc gggcgtttcg gcagcccggc ggctatcttg tccgcgccat
      361 gtccatcgcc cgctcattcc tgctccgcgc ctcccgcagc ccgtggctcg ccgagcagtt
      421 ccgccggcgc gcgttcgccc gtcgcgccgt acgccgcttc atgccgggcg agagcctgct
      481 ggatgcgctc gacgcatccg ccgagctcgc caaggcgtcg atgggaaccg tcctgaccca
      541 gctgggagag caggtgcgca acgccgccga ggccgaagac gtgcgcgacc actacctcaa
      601 cgtgttcgac caggtgcgcg accggtttct cccggcgcag gtatcggtga aactgacgca
      661 tctcggcctc gacgtggacc gggacacctg cctgcgccac gtgcgcgcgc tcgccgagaa
      721 ggcccggaaa gagggatgct tcctgtggat cgacatggag gaatcccgct atgtggacgc
      781 cacgctcgat ctctaccgcc gcgtacggca agaaggcccc ggcgtggggg tgtgtctcca
      841 ggcctatctg cggcgcacgc cgcaggacct cgcggccctg ctgcccctgt cgcccgcgat
      901 ccgcctcgtg aagggcgcct acaacgaacc cgggacggtg gcgttgccgc gcaagacgga
      961 cgtggatgcg cagtactcca cgctcgccgt ccagctgctc gacgccgcgg ccgccggcct
     1021 cgccaccacg gtcttcggca cgcacgacct cccgctgatc gagcgcatcc gggagcaggc
     1081 ggccgcacga agggccgatg gacgctacga ggtgcacatg ctgtacggca tccgcagcga
     1141 cgcgcagcgc cggctcgccg gccagggtgt gaaggtgcgg gtgctgatca gctacggctc
     1201 acactggttc gcctggtaca tgcggcgcct cgccgagcgg ccggcgaacg tgtggttcgt
     1261 ggtgcggagc ctcttctagc cgagcacctt gcggctcagc gcccggccga tgaaccgcag
     1321 gcgctccggc caggagaacc gcagcaggtt cgtcttcacc acccccgccg tgaagaaggt
     1381 cttgcgcgag tagtcgatcg ccacgtccac cagcaccggc cggcccacag cgaggatctc
     1441 gcgcacccgg gccaggacag tcgcgatatc cgcatcgcgc gccagggtta ccgcctcgac
     1501 tcccagcgcc ccggcgagcg ccccggcgtc gtaatcggga agcacgctcg ccgtcttgcg
     1561 ggagagcgcc gtcgcctgga attgcgcgat ttgcgccagc tcacgatcgc gcagcacgaa
     1621 gaccgccacc gcgatcccca gctgcgcggc cgtgagcagc tcgagccccg tcatgagcag
     1681 cgccccgtcc cccgcgagcg ccacgaccgg acactccggc cgcgcgagct tcgccccgag
     1741 tgccgcgggc accgcgtacc ccatgcacga gtagtccacc ggcgccagga accggcccgg
     1801 acactcgagg cgcaggcact ccatcgcgag gaacgttccg ttgccgctgt cggtcgagaa
     1861 gaccgtctcc ggtccgaacg cctcctgca
//
LOCUS       DBNGZW010000538         2406 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_662770, whole genome shotgun sequence.
ACCESSION   DBNGZW010000538 DBNGZW010000000
VERSION     DBNGZW010000538.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2406)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2406)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2406
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_662770"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="AC16JC_12805"
     CDS             <1..628
                     /locus_tag="AC16JC_12805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165622.1"
                     /translation="RGSPDQPYKFPPLHHLAPPRDGAILRPPTHRWKAHARMQFQARI
                     EPLDGKLPKVSYRWDPETDILSATCKGVPKASGMDGSVDLEGSDGSFVLLDVAGGLLR
                     GLDVVTWPDDIRTVDALAAPTAAEEARVLFPSRRSQPAVAAVEVDTALTVEKNAAESV
                     IHIRVGRTRPVSVVRVADLLLVEVDKQSRLAGLWLLEVPPFPNVEATA"
     gene            629..1267
                     /locus_tag="AC16JC_12810"
     CDS             629..1267
                     /locus_tag="AC16JC_12810"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR5165623.1"
                     /translation="MEDERLQRYIEELFAVEDSVLKSVRERHAELDLPPIHISPDEGK
                     LLHVLLRAAGARTVLEIGSLAGYSGIWLARALPVGGTLTTIEKHPVHAELARQAYAEA
                     GVAERVRLVEGEALSVLETLPPAAFDAVFVDADKEPLPEYFEWSVRLLRLGGLLLCDN
                     AFFHGSVVDPNDHSPQAEGVRAFNRLAAIDRRVVSAVIPIRDGVVIGVKVAP"
     gene            1264..2271
                     /locus_tag="AC16JC_12815"
     CDS             1264..2271
                     /locus_tag="AC16JC_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165624.1"
                     /translation="MSEGRRDWLTALYAAGVTPASFLPDASAGRATDPDAAPAPTVEL
                     VEDDELQVHALVPGPPPAAVAFLDGIQQWKVVGYDGVRPVAVAYVAAAVRQRGRDGRL
                     RTALWEARELVLAAPESLDDARRRAIEGAGLEVVPAEADAAGAGQPGQALETLRREVR
                     RARQNLERDLGRRAVEQLAGDEWLVVDGQLAISAVLARHPRAIGVIKSHGAQFLEGRH
                     LERALTVPAVHRTSVFRVHGGHGRTEVDSWYLRLWPWEGRDLYYGLLRIEVARGPDPG
                     ARAPGISGWLCAERATLSAPDARFDRLLYPIHDVETYLRSRAPGDLLASPASRLPRTG
                     T"
BASE COUNT          338 a          837 c          875 g          356 t
ORIGIN      
        1 ccgcggctcc cccgatcaac catacaaatt cccgccgctc catcatctgg ctcctccgcg
       61 agacggggct atattacgcc cccccacgca ccggtggaag gcgcacgcgc gcatgcaatt
      121 ccaggcgagg atcgagccgc tcgacggcaa gctccccaag gtgtcctacc gttgggaccc
      181 ggagaccgac atcctgtcgg ccacctgcaa gggcgtgccc aaggccagcg gcatggacgg
      241 cagcgtcgat ctcgagggga gcgacggatc gttcgtcctg ctggacgtgg cgggcggcct
      301 gctccgcggg ctggacgtcg tcacctggcc ggacgacatc cgcacggtcg atgccctcgc
      361 ggcgcccacc gcggccgagg aggcccgcgt gctgttcccg tcgcggcgct cccagccggc
      421 ggtcgcggcg gtcgaggtgg acacggccct gacggtcgag aagaacgcgg cggaatcggt
      481 gatccatatc cgcgtcgggc gcacgcgccc ggtcagcgtg gtgcgcgtcg ccgatctgct
      541 cctcgtcgag gtggacaagc agtcccgcct cgccggcctg tggctgctcg aagttccgcc
      601 gttccccaac gtcgaggcca ccgcctaagt ggaggacgag cggctccagc gctacatcga
      661 ggagctgttc gcggtagaag attctgtcct caagagcgtg cgcgagcgcc acgccgagct
      721 cgacctgccg ccgatccaca tttccccgga cgaggggaag ctgctgcacg tgctgctgcg
      781 ggccgccggc gcgcgcaccg tgctggagat cggctcgctc gcgggctact ccgggatctg
      841 gctggcccgc gcactcccgg tcggcggtac gctcaccacg atcgagaagc acccggtgca
      901 tgcggagctg gcgcgccagg cgtacgccga ggctggagtg gccgagcggg tcaggctggt
      961 ggagggcgag gcattgagcg tgttggagac gcttccgccg gcagcgttcg acgccgtctt
     1021 cgtggacgcc gacaaggagc cgctcccgga gtatttcgag tggagcgttc ggttgctgcg
     1081 actggggggt ctgcttttgt gcgacaatgc gttcttccac gggtcggtcg tggacccgaa
     1141 cgaccattcg ccgcaggccg agggggtccg cgcgttcaac cggctcgcgg cgatcgaccg
     1201 gcgtgtggtc tcggccgtga ttcccatccg cgacggcgtc gtaatcgggg tgaaggtcgc
     1261 cccatgagcg aaggacggcg cgactggctc accgccctgt acgccgccgg ggtcaccccc
     1321 gcttcctttc ttcccgatgc gagcgccggc cgggcgaccg accccgacgc ggcgcccgcg
     1381 ccgacggtcg agctggtaga ggatgacgag ctgcaggtgc acgccctcgt ccctggccca
     1441 ccgcccgccg cggtcgcatt tctcgacggc attcaacaat ggaaggtggt cggctacgac
     1501 ggggtccggc ccgtcgccgt ggcatacgtc gcggcggcgg tgcggcaacg gggccgggac
     1561 ggtcggctgc gaaccgcgct atgggaagcg cgcgagctgg tcctcgccgc accggagtcg
     1621 ctggacgacg cacggcgccg cgcgatcgag ggggcgggcc tggaggtggt gcccgccgaa
     1681 gccgacgcgg caggggcagg gcagccgggg caggcgctcg agacgctgcg tcgcgaggtg
     1741 cggcgcgccc gccagaatct ggagcgcgac ctgggtcggc gcgcggtcga acaactggcc
     1801 ggggacgagt ggctggtcgt ggacgggcag ctggcgatct ccgccgtcct cgcgcggcat
     1861 ccacgcgcca tcggcgtcat caagagccac ggcgcgcagt tcctcgaggg gcggcatctc
     1921 gagcgcgcgc tcaccgttcc cgcggtgcac cgcaccagtg tgttccgcgt gcacggcggg
     1981 cacggccgca ccgaagtcga ttcctggtac ctgcggttgt ggccctggga aggccgcgac
     2041 ttgtactatg ggttgctgcg gatcgaagtg gcgcggggcc cggaccccgg ggctcgggcc
     2101 ccagggatct ccgggtggct ctgtgccgag cgagcgacgc tctcggcgcc cgacgcgcgc
     2161 ttcgaccggt tgctgtatcc tatacatgac gtggagacct atctgagaag ccgcgctccc
     2221 ggggatctcc tggcctcgcc ggcgtcccgg ctgccgcgca ccggcacgtg atcgaacgac
     2281 cttcgcagcg ccccggcgag cccggcccgg accccatcgg tcgggtcgtc gccacggaac
     2341 agaagcccgc cacgccgcac cagttccact tctggacggc gacggagacg accgtcggga
     2401 tcgggg
//
LOCUS       DBNGZW010000539         2550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_447046, whole genome shotgun sequence.
ACCESSION   DBNGZW010000539 DBNGZW010000000
VERSION     DBNGZW010000539.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_447046"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="AC16JC_12820"
     CDS             complement(<1..782)
                     /locus_tag="AC16JC_12820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5165625.1"
                     /translation="MRRPLSPPLGGLTAAVLVTLLAGCDARKSGTETASATLTDSTAA
                     TVPTGGAVPVSSASDEARDLYLKGRALAEQLRAHDGRELFAEAAAKDPAFAMAHYQLA
                     ANAATAKDFFAHLGEAVALADRVSEGERLMILALQAGGNANPAKQLEYQQELVAKCPN
                     DERARFLLGGGWFGRQEYDKAIEQYRKSTEINPGFSPAYNLLGYAYRQVEKYPEAEAA
                     FKKYIELVPGDPNPYDSYAELLMKTGRFDESIAQYRKALEIEP"
     gene            complement(877..1428)
                     /locus_tag="AC16JC_12825"
     CDS             complement(877..1428)
                     /locus_tag="AC16JC_12825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR5165626.1"
                     /translation="MTYMECPQCGLKALSVATRCPHCGVAFPSRPLRRPASSSGLDRL
                     RPLLALVGVVAVAFAMVAVIMPRLQSKPGSGAPPPGEVIAAPVMVAPDSVPVAVPPAV
                     PPTVSTAPAAVAPGVRGLRYARTWVNVRSARSRTAPSVLVLQPADSMQVDSLSGGWYR
                     VLIAGRPIGYAHRSNLDSVPPQL"
     gene            1616..>2550
                     /locus_tag="AC16JC_12830"
     CDS             1616..>2550
                     /locus_tag="AC16JC_12830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluzincin family metallopeptidase"
                     /protein_id="HIR5165627.1"
                     /translation="MPYALRGFAFRVLLLACAWSGTTWAQERAFTHADTVRGSITPER
                     AWWDVAFYDLHVRLDPADSTVRGWNGITYRVTGPSRAMQIDLQTPLEVDSMVQDGRRL
                     TYRRDGNAFLVTLAAKQPKGAVKTVTVYYHGRPIVAERPPWDGGLIWARDPDGRTWIS
                     TACQGLGASVWWPTKDTQADEPDSQRVAITVPDSLQAIGNGRLRGIERAGDGWTTWEW
                     FVASPINNYDVAPYVGRYAQFTDTYFGEGGLLTLDFRPLAAHLEAAREQWKQVKPMLQ
                     CFENWFGPYPWYHDGYRLIEAPHLGMEHQSAVAYG"
BASE COUNT          404 a          869 c          895 g          382 t
ORIGIN      
        1 ggctcgatct ccagcgcctt gcgatactgg gcgatcgatt cgtcgaaccg cccggtcttc
       61 atcagcagct cggcatacga gtcgtaggga ttgggatcgc cgggaacgag ctcgatgtac
      121 ttcttgaacg cggcctccgc ctcgggatac ttctcgacct gccggtacgc gtagccgagg
      181 aggttgtagg cgggggagaa gccaggattg atctcggtgg acttgcgata ctgctcgatc
      241 gccttgtcgt actcctgccg gccgaaccag ccgccgccga gaagaaagcg ggcccgctcg
      301 tcgttggggc acttggcgac gagctcctgc tggtactcga gctgcttggc cgggttggcg
      361 ttcccgccgg cctgcagtgc caggatcatc agccgctcgc cctcggacac ccggtcggcg
      421 agcgccaccg cctcccctag atgcgcaaag aaatccttgg ccgtggcggc gttggcggcg
      481 agctgatagt gcgccatcgc gaacgcggga tccttcgcgg ccgcctcggc gaacagctcg
      541 cggccgtcgt gcgcgcggag ctgctcggca agcgctcgtc ccttgagata gagatcgcgc
      601 gcctcgtcgg aggcgctgga aaccggcacc gcgccgccag tgggcaccgt cgcggcggtg
      661 gagtcggtga gggtggcact cgcggtctcg gtgccggatt tcctggcatc gcagccggcg
      721 agcagggtga cgaggacggc ggcagtgagg ccaccgagtg gtggcgagag gggacggcgc
      781 atcggatggt cctccggcgg cgttgctatt ggagacagct gggtgcgtct gagagggagc
      841 cctacctaag acctacgccg gaggtgcgga gcggtttcag agctggggag gaacggagtc
      901 caggttggag cggtgggcgt acccgatcgg ccgcccggcg atgagcacgc gataccatcc
      961 tccggacagc gagtcgactt gcatcgagtc ggcgggctgg aggacgagaa cggacggcgc
     1021 cgtgcggctg cgcgcgcttc ggacgttgac ccaggtccgg gcatagcgca ggccccgcac
     1081 gcccggtgcg acggctgcgg gcgcggtgga gaccgtcggt ggcacggccg gcggaacggc
     1141 gacgggtacg gagtcgggcg cgaccatcac cggcgccgcg atcacttcgc ccggcggagg
     1201 cgcgccggaa ccgggcttcg actgcagccg aggcatgatc accgcgacca tcgcgaaggc
     1261 cacagccacc acgccgacga gggccagcag cggccgcagc cggtcgagcc cgctgctcga
     1321 agcgggtcgc cgaagcggcc gcgacggaaa tgctacgccg cagtgcggac accgggtcgc
     1381 gacgctcagc gccttcagac cgcactgcgg acattccatg taggtcatag cgcctcgggg
     1441 agtggcgccg tccaagatgc tgcgcattcg gcgaggccgc caggattgaa tccgcccggc
     1501 ccgcgccccg tagtgccaac ctgtgcaccg ccgcggactt tccgctcccg agcagcccgt
     1561 ataggatacc ccgcgtcacg atgctgcccg ctcactcctg cctccacgga tgatcatgcc
     1621 gtacgccctg cgtggctttg ccttccgggt cctcctgctc gcctgcgcct ggtcgggcac
     1681 cacgtgggcc caggagcgcg ccttcaccca tgccgatacg gttcgtggaa gcatcacgcc
     1741 cgagcgggcg tggtgggacg tcgccttcta cgacctccac gtgcggctcg accctgcgga
     1801 cagcacggtt cgcggctgga acgggatcac ctatcgggtt accggtccgt cccgcgcaat
     1861 gcagatcgac cttcagacgc cgctcgaggt cgacagcatg gtgcaggacg gcaggcggct
     1921 cacgtaccgg cgcgacggca atgcgtttct cgtcacgctc gccgcgaagc agccgaaggg
     1981 cgccgtcaag accgtaacgg tctactacca cggccggccg atcgtcgcgg agcggccgcc
     2041 gtgggacggc gggctcatct gggcacgcga tccggacggc cggacctgga tctccaccgc
     2101 ctgccaggga ctcggcgcca gtgtctggtg gcccaccaag gacacccagg ccgacgagcc
     2161 cgacagccag cgggtggcca tcacggtccc cgactcgctc caggccatcg gcaacggccg
     2221 cctccgtggc atcgagcgcg cgggcgacgg ctggaccacc tgggagtggt tcgtcgccag
     2281 cccgatcaac aactacgacg tcgcgcccta cgtcggccgc tacgcgcagt tcaccgacac
     2341 ctatttcggc gagggggggc tgctcacgct cgacttccgg ccgctggccg cgcacctcga
     2401 ggccgcccgc gagcagtgga aacaggtgaa gccgatgctc cagtgcttcg agaactggtt
     2461 cggcccctat ccctggtacc acgacggtta ccgcctgatc gaggcgccgc acctgggcat
     2521 ggaacaccag agcgcggtgg cgtacggcaa
//
LOCUS       DBNGZW010000540         4177 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1143103, whole genome shotgun sequence.
ACCESSION   DBNGZW010000540 DBNGZW010000000
VERSION     DBNGZW010000540.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4177)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4177)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4177
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1143103"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..562)
                     /locus_tag="AC16JC_12835"
     CDS             complement(<1..562)
                     /locus_tag="AC16JC_12835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165628.1"
                     /translation="MKVERAGRWQKALRLLGPVVATSILAAAPAGAADNTTAGTATAL
                     VSGCAQITVTAPFTGDANGNGSVLVEYNTTNSWPGTTACGAVGGPSPRMCLITGLNAS
                     TAYYVRATYSDPEGVSGAAVQVLPGTYTTTACSGQGGSPMILILTPARDAVIGGTSTT
                     FQVQVYGPDSIATASILWGLDGSTPTN"
     gene            complement(559..1653)
                     /locus_tag="AC16JC_12840"
     CDS             complement(559..1653)
                     /locus_tag="AC16JC_12840"
                     /inference="COORDINATES: protein motif:HMM:NF021235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5165629.1"
                     /translation="MTRHRVLLCVALVALGALLAIVARAGDEPHWATFTVQCASCHAT
                     HKAAGGNLTSVAGNENLCASCHNVSGGLASTFPIERMNKANLVAETGSSHAWGVSATN
                     SAAGATPPSNTAMSVRLDSGNIVCSTCHNQHFSPSGAVTAGTAGTQILGTVAHVLGTG
                     TGTVSYTAAAAASPKGYILEIIETGGAAGTAKFRLSNDGGTSWWGYSGGNWVAYAPGN
                     ARLTGSNVLVNDSSNVSVTFTGATSGSFVLGDRWRFYVGYPFLRLPLDTASSNFCRDC
                     HASWTMNHGAVESWTGALRSHPVGEALNANGGNYDRSTPLDANGVPQAGDRDGNPTND
                     LKLGQDGTVQCFTCHGLHHADGNSQTVDTP"
     gene            complement(1650..>4177)
                     /locus_tag="AC16JC_12845"
     CDS             complement(1650..>4177)
                     /locus_tag="AC16JC_12845"
                     /inference="COORDINATES: protein motif:HMM:NF012271.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HIR5165630.1"
                     /translation="GWLPLNGGRATRAGALLDGRLAVVDAVGGSVSAVSTSGSVSWRV
                     GGFSQPIAAAATASELYVLDRGAQRISVLSTGGSALRTIPCPGQMPSGLAIGPGAGEL
                     TVSDMAAARLHVISAATGQPLRSMGEGVLSFPSGVTSPPGRVAVADPGLRRVVIFDST
                     GNLLAILGDDTLFVRPQGVAWDGTGNRLVVSDSFAAELTVMGEDGTVRGQLAGFGSGA
                     GQISAPVDVQVVGGGLVAVPLAVSGQVALYQLLSSLPPVAPPTGVNAGDLTGDDGGAI
                     QVNWTKSADDPGRVVSYRIDRATGPSGDFVVYASAAAGSTSYVDRATIDGQCYRYRVV
                     ASDGVGEGISEGTSCAVSANDLPPPAPAFASAQAQSPTTALVTWAPVAAPDLASYALQ
                     VAGPERTWTLEAGAGSAQVSLTNLLPDQAYHVSVAAVDTAGNVSPPALASFTTYPDEA
                     PPTPTNVAASDTRRGGEIEVTWTGDAGRVPVAGFHVVCTPQIPGWPTVEADAAQSPAR
                     LSGLINRLAYFVTVTAVTPWGRSSAPSTGTGVMPTVPPRDLPVLPEVGWPADRPDADA
                     GGITVRIPPQSVDRNLRLEYRARNSQMLVFLDGAPLATNFGDTAGVFANADIALPATL
                     LGGTSVHLLELRSAAFPDPSAVFSLRLTDLVPLPVEGLQSVPFNTVVDLTWSWAEPHA
                     GRTVKVSHSVNGPGGEWQDVPCTASSLARCRDTFRPNGQKTWYRVTVVSPAGWESPKQ
                     GTTGKSAYSAGPPPVTDLLVQPIDSSSGGWALTWTPLSTALSPSADPSSVSSYRVYRQ
                     DPGGLVFLVEVPARTVQLPADALDAAGTNAVVHSVDALGRESP"
BASE COUNT          644 a         1385 c         1513 g          635 t
ORIGIN      
        1 cgttcgtcgg ggttgagcca tcgaggcccc agaggatgct cgcggtcgcg atgctgtccg
       61 gcccgtagac ctgcacctgg aacgtcgtgc tcgtgccgcc gatgacagcg tcgcgcgccg
      121 gcgtcagaat gagaatcatc ggcgatccac cctggccgga gcaggcggtc gtcgtatacg
      181 ttccgggaag gacctgcacc gccgccccgc tcacgccctc ggggtcgctg tacgtcgccc
      241 ggacgtaata cgccgtggac gcgttcagac cggtaatcaa gcacatcctg ggcgaggggc
      301 ccccgaccgc gccgcacgct gtcgtgcccg gccacgagtt cgtcgtgttg tactcgacga
      361 gaacggatcc gtttccgttg gcgtcgccgg tgaatggagc cgtgacggtg atctgagcgc
      421 agccgctcac gagcgccgtg gcggtccccg ccgtcgtgtt gtctgcggcg ccggctggag
      481 ccgcggcaag aatcgaagtg gcgacaaccg gtccgaggag ccggagcgct ttctgccagc
      541 gacctgccct ttcgactttc atggcgtgtc caccgtctgc gagttgccgt cggcgtggtg
      601 caggccgtgg caggtgaagc actggaccgt gccgtcctgc ccgagcttga ggtcgttcgt
      661 gggattgccg tccctgtcgc cggcctgcgg cacgccgttg gcgtcgagcg gagtggagcg
      721 gtcgtagttc cccccgttcg cgttgagggc ctcgcccact ggatggctcc tgagggcacc
      781 cgtccaggac tcgactgcgc cgtggttcat tgtccaggat gcgtggcagt cgcggcagaa
      841 gttggaagag gcggtgtcga gtgggagcct gaggaacggg taaccgacgt agaaccgcca
      901 cctgtcgccc aggacgaagg agccgctcgt ggcgccggtg aaggtcaccg agacgttgga
      961 gctgtcgttg accaggacgt tcgagcctgt gagccgggcg ttccccgggg cgtaggccac
     1021 ccagtttccg cccgagtagc cccaccagga tgttccgccg tcgttcgaga gtcggaattt
     1081 cgcggtgccc gcggctccgc cggtctcgat gatctccaga atgtagccct tcgggctggc
     1141 ggcggccgct gccgtgtagg aaacggtccc cgtgccggtt ccgaggacgt gcgccaccgt
     1201 gccgagaatc tgggtcccgg ccgtgccggc ggtcacggcg cctgacgggc tgaagtgctg
     1261 gttatggcac gtggaacaga cgatgttgcc cgagtcgagg cgcacgctca tggcggtgtt
     1321 ggatggaggc gtggcgccgg cagcgctgtt cgtggcgctg acgccccacg cgtgcgacga
     1381 gccggtctcg gcgacgaggt tcgccttgtt catcctctcg atcgggaagg tagacgcgag
     1441 gccgccgctc acgttgtggc aggaagcgca gaggttctcg ttgccggcga ccgaggtgag
     1501 attgcctccc gccgctttgt gggtcgcatg gcaggacgcg cattgaacgg tgaacgtcgc
     1561 ccagtggggc tcgtcgccgg ccctggcgac gatggcgaga agagcgccga gcgcgacgag
     1621 cgcgacgcag agaaggacgc ggtgtcgggt catggcgact ctctcccgag ggcgtcgacc
     1681 gagtggacga cggcgttcgt tccggccgca tcgagggcgt cggcggggag ctgaacggtg
     1741 cgcgccggga cctccacgag gaagacgagc ccccccggat cctgacggta gacgcgatac
     1801 gagctgactg aagaaggatc agccgaggga ctgagcgcgg tggagagggg ggtccaggtc
     1861 agagcccatc ctccgctcga ggagtcgatc ggctggacga ggagatcggt gactggcgga
     1921 gggccggcgc tgtaggcgct cttgccagtg gtgccctgtt tcggcgactc ccagccggcg
     1981 ggggacacga cagtaacgcg gtaccacgtc ttctggccgt ttggccggaa cgtatcccgg
     2041 caccgggcaa gcgaggatgc cgtgcagggc acgtcctgcc attcgccgcc gggcccgttc
     2101 acggagtgcg acaccttcac cgtccggcct gcgtggggct cggcccagct ccacgtcagg
     2161 tccacgaccg tattgaaggg aaccgactgg aggccctcga cgggcagcgg gaccaggtcg
     2221 gtcagccgca gggagaacac ggccgaggga tcggggaagg ctgcactgcg gagctccaat
     2281 aggtgaacgg acgttcctcc caggagcgtc gcgggcagcg cgatgtcggc attggcgaag
     2341 acacccgctg tgtcgccgaa gttggtggcg aggggcgccc cgtcgagaaa gacgagcatc
     2401 tgtgagtttc gggcgcggta ctccaggcgc agattgcggt caacgctctg gggcgggatg
     2461 cggacggtga tcccgcctgc gtccgcatcg ggccggtcgg ccggccagcc cacctccggg
     2521 aggaccggca ggtctctggg cgggaccgtc ggcatcaccc cggtgccggt ggacggcgcg
     2581 ctcgatcggc cccacggggt cacggccgtg accgtgacga agtaagcaag cctgttgatc
     2641 aggccactca acctcgcagg cgactgcgcc gcgtcggcct cgacggtggg ccagccggga
     2701 atctgtggtg tgcagacgac gtggaagccc gccacgggga cgcgccccgc gtccccggtc
     2761 cacgtcactt cgatctcgcc tccccgtcgc gtatccgacg ccgcgacatt cgtgggagtc
     2821 ggcggcgcct cgtcgggata ggtggtgaag ctcgcgagcg ccggtggcga aacattcccc
     2881 gctgtgtcga cggcggcgac cgagacgtgg taggcctgat cgggcaagag gttcgtcagc
     2941 gaaacctgtg cgctgccggc accagcctcc agcgtccacg ttcgctccgg tcccgcgacc
     3001 tgcagggcgt agctggcaag gtcgggcgcg gccaccggcg cccacgtgac cagcgccgtc
     3061 gtcgggctct gcgcctgggc ggaggcgaag gccggggctg gtggcggaag gtcgtttgcc
     3121 gacacggcgc aggacgtccc ctccgaaata ccctcgccga cgccgtccga cgccacgacg
     3181 cggtagcggt agcactggcc gtcgatcgtt gcccggtcga cgtacgaagt ggagccggca
     3241 gccgccgagg cgtagacgac gaagtcgccc gaaggacccg tggctctgtc gatccggtag
     3301 gagacgacgc ggccggggtc gtcggccgat ttcgtccagt tgacctggat cgctccgccg
     3361 tcgtctccgg tgagatctcc cgcgttgacg cccgtcggag gcgccaccgg cggaaggctg
     3421 gacagcagct gataaagcgc gacctggccc gagaccgcca gcggcaccgc cacgagaccg
     3481 ccgcccacga cctggacgtc caccggcgct gagatctgac ccgctccgct tccgaagccg
     3541 gccagctgtc cccgcaccgt gccgtcctcc cccatcacgg tcagctcggc cgcgaagctg
     3601 tcggagacga cgaggcggtt gccggttcca tcccacgcga ccccctgcgg cctcacgaag
     3661 agcgtgtcgt ccccgaggat ggccagcaga tttccggtcg agtcgaagat caccacgcgg
     3721 cgcaggccgg ggtccgcgac cgcgacccgg cccggcggag aggtcacgcc ggaaggaaag
     3781 ctgagaacgc cctcgcccat ggatcgcagc ggctggcccg tcgccgcgga aatgacgtga
     3841 agcctggccg ccgccatgtc gctgacggtg agctcgccgg cgcccggacc gatggcgagg
     3901 ccggacggca tctggcccgg gcacgggatc gtgcggagcg ccgatccgcc ggtgctcagc
     3961 acggagatcc gttgcgcacc gcgatccagg acgtaaagct cgctcgccgt cgcggcggcc
     4021 gcaatgggct gcgagaaacc gccgactctc caggaaacgc tccctgacgt tgagaccgcc
     4081 gacacggaac cgcccaccgc atccaccacc gcgaggcggc cgtcgagcag cgcaccggcg
     4141 cgtgttgctc gcccgccgtt caaggggagc catccgt
//
LOCUS       DBNGZW010000541         2852 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_106935, whole genome shotgun sequence.
ACCESSION   DBNGZW010000541 DBNGZW010000000
VERSION     DBNGZW010000541.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2852)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2852
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_106935"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="AC16JC_12850"
     CDS             complement(<1..386)
                     /locus_tag="AC16JC_12850"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF025118.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR5165631.1"
                     /translation="MGSSLDQFAAFSPEGAWTVAEVTRRARAVVEGGLPPLWVKGEVS
                     GFKAYGSGHWYFTLRDRVAQLRCVMWSRDNQRLPAPPADGMQVYAFGSPTVWEERGEF
                     RLTARHLLSTEAGGLWQVAFEKAKAAL"
     gene            complement(371..1264)
                     /locus_tag="AC16JC_12855"
     CDS             complement(371..1264)
                     /locus_tag="AC16JC_12855"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015504095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HIR5165632.1"
                     /translation="MHASAKLIDGNAIGQAMRPELAGEIAALKAQGVTPGLAAVLVGE
                     NPASATYVRMKGKACDEAGLYHETIDRPADISEADLLALVDRLNADPKIHGILVQLPL
                     PKHINTGRVLLRIDPRKDVDGFHPTNVGKVSSGDASGFRPATPYGVQQLLLRSGVETT
                     GAHAVIVGRSNIVGRPMAALLLQDGPGGNATVTVCHSRTRNIGAVTRQADILIVAMGK
                     PQFVTGDMVRPGAVVIDVGVNRVDDPGAKRGYRLVGDVKFDEVAPLASAITPVPGGVG
                     PMTITMLLVNTVQAARQWARP"
     gene            complement(1254..2768)
                     /gene="rny"
                     /locus_tag="AC16JC_12860"
     CDS             complement(1254..2768)
                     /gene="rny"
                     /locus_tag="AC16JC_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019005394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HIR5165633.1"
                     /translation="MSTWIEVVLSVLGGGLVAGTAFYVMGRRVERRVAEATARLSAGE
                     RERQLADARARVELEAREELVRTREQLEQDLAERRREVDRRLTALEKTQTATAERERE
                     IVRREQAFTERDRALTGREQEARAQLERLAGLSAQDAKEELKRRLEEEARGEAAALLR
                     DIREQAQKSADREAKKIVALAIQRIAADHTAETTASAVALPSDEMKGRIIGREGRNIR
                     AFEAATGTDVIIDDTPDTVVISCFDPVRREVARLSLERLISDGRIHPGRIEEVVRKAQ
                     EEIEAGIVEAGERAVYETGLRGMHQELVKLVGRMRYRTSYGQNILEHSKEVAWMAGIM
                     AAELKLDVQLAKRGGLLHDIGKVLTHEHDGTHVQLGVEVATKYGEHPVVINCIAAHHD
                     DVPHDSAISVIVQAADAVSGSRPGARREAFETYVKRLTAIEQIAAEFSGVEKVYAIQA
                     GREVRVVVAPGAVDDAKATGLAEQISRKIERELQYPGQIKVVVIRETRAVDFAR"
BASE COUNT          387 a         1050 c          934 g          481 t
ORIGIN      
        1 agcgccgcct tggctttctc gaaggccacc tgccacagac cgccggcttc ggtggagagc
       61 aggtgccgcg cggtgagccg gaattccccg cgctcttccc agacggtcgg cgagccgaag
      121 gcgtacacct gcatgccgtc ggcgggcggc gcgggcaggc gctggttgtc gcgcgaccac
      181 atcacgcagc gcagctgggc gacgcgatct cgcaaggtga agtaccagtg tccgctgccg
      241 taggccttga agcccgagac ttcgcccttc acccacagcg gcgggagccc accttcgacg
      301 acggcgcgtg cccgccgcgt cacctcggca acggtccagg cgccttcggg tgagaacgcg
      361 gcgaactggt ctagggacga gcccattgcc gcgcggcctg gaccgtgttg acgagcagca
      421 tcgtgatcgt catgggaccg acgccgccgg gcacgggcgt gatggcggat gcgagcggcg
      481 ccacctcgtc gaacttcacg tcccccacga ggcggtatcc ccgcttggcc ccggggtcgt
      541 ccacgcgatt gacccccacg tcgatcacga cggcgcccgg gcggaccata tcgcccgtga
      601 cgaattgcgg cttgcccatc gcgacgatca ggatgtcggc ctggcgcgtc accgccccga
      661 tgttgcgcgt gcgggagtgg cacaccgtca cggtggcgtt gccgccgggg ccgtcttgca
      721 gcagcagtgc cgccatgggg cggcccacga tgttggagcg ccccacgatc accgcgtgcg
      781 cgccggtcgt ctccacaccg ctgcgcaaca gcagttgctg gaccccgtag ggagtggcgg
      841 gccggaaccc cgacgcatcg ccgctcgaga ccttgcccac gttggtcggg tgaaagccgt
      901 ccacgtcctt gcgaggatcg atgcgcagca gcacgcggcc ggtgttgatg tgcttgggca
      961 agggcagctg gaccaggatg ccgtggatct tcggatcggc gttgagccgg tcgacgagcg
     1021 ccagcaggtc tgcttcgctg atgtcggccg gccgatcgat cgtctcgtgg tacaggccgg
     1081 cctcgtcgca cgccttgccc ttcatgcgca cgtaggtggc cgacgcgggg ttctcgccca
     1141 ccagcaccgc cgccaggccg ggcgtcacgc cctgggcctt gagtgcggcg atttctccgg
     1201 ccagctcggg ccgcatcgcc tggccgatgg catttccgtc gatgagtttc gcgctagcgt
     1261 gcaaagtcca ccgcccgtgt ctcccggatg acgaccacct tgatttgccc cggatactgc
     1321 agttcccgct cgatcttgcg ggagatctgc tcggccaatc ccgtggcctt ggcgtcgtcc
     1381 accgcccccg gggcgacgac gacgcggacc tccctccccg cctgaatcgc gtacaccttc
     1441 tccacgcccg agaattccgc ggcgatctgc tcgatcgccg tgagccgctt gacgtaggtc
     1501 tcgaaggcct cgcggcgcgc cccgggccgc gagcccgaca cggcatccgc cgcctgcacg
     1561 atgacgctga tcgcggagtc gtgagggacg tcgtcgtgat gcgccgcgat gcagttgatg
     1621 accaccggat gctcgccgta cttcgtggcg acctccacgc ccagctgcac gtgcgtgccg
     1681 tcgtgctcgt gggtgagcac cttgccgatg tcgtgcaaca aacccccgcg tttcgcgagc
     1741 tggacatcga gcttgagctc ggccgccatg atgcccgcca tccacgccac ttccttcgag
     1801 tgctccagga tgttctgccc gtaggaggtc cggtagcgca tccggccgac gagcttgacc
     1861 agctcctgat gcatgccgcg caggccggtc tcgtagaccg cccgctcccc cgcctccacg
     1921 atgccggcct cgatctcctc ctgcgccttg cggacgacct cttcgatgcg tcccgggtgg
     1981 atgcggccgt cgctgatgag ccgctccagc gacaggcgcg cgacctctcg gcggaccggg
     2041 tcgaagcagg aaatcaccac ggtatcgggg gtgtcgtcga tgatgacgtc cgtgccggtg
     2101 gcggcctcga acgcgcggat gttgcggcct tcgcgcccga tgatccggcc cttcatttca
     2161 tccgagggga gcgccacggc tgacgccgtc gtctccgccg tgtggtcggc ggcgatgcgc
     2221 tgaatggcga gcgccacgat cttcttcgcc tcgcgatcgg cgctcttctg ggcctgctcg
     2281 cggatgtccc gcagcagggc ggccgcttca ccgcgcgcct cctcttccag gcgccgcttg
     2341 agctcttcct tcgcgtcctg agccgagagc cccgcgagcc gttcgagctg ggcgcgtgcc
     2401 tcctgctcgc gccccgtgag ggcccggtcg cgctcggtga aagcctgttc ccgccggacg
     2461 atctcccgct cccgctcggc ggtcgcggtc tgggtctttt ccagcgcggt caggcgccgg
     2521 tccacttccc ggcgacgctc cgccagatcc tgctcgagct gctcacgggt ccggaccagc
     2581 tcctcgcgtg cctcgagctc gacccgcgca cgcgcatccg ccagctgacg ctcccgctcg
     2641 ccggccgaca gtcgagcggt cgcttcggcc acgcggcgct ccacgcgccg gcccatcaca
     2701 tagaaagccg ttcccgcgac gagtcctccg ccgagaacgg acaggacgac ctcgatccac
     2761 gtagacatcg atctatctcc ggcaacgcct cgacccatgc gggccgggcg acagtcaccc
     2821 gcgctcgacc gccttgcgcc gtccgggcgg ca
//
LOCUS       DBNGZW010000542         6784 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_596764, whole genome shotgun sequence.
ACCESSION   DBNGZW010000542 DBNGZW010000000
VERSION     DBNGZW010000542.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6784)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6784)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6784
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_596764"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..111
                     /locus_tag="AC16JC_12865"
     CDS             <1..111
                     /locus_tag="AC16JC_12865"
                     /inference="COORDINATES: protein motif:HMM:NF049034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CysS/YqeB C-terminal domain-containing protein"
                     /protein_id="HIR5165634.1"
                     /translation="ARGTRDFTEADRIREELRARGIEIEDTPGGTKWRRS"
     gene            complement(108..662)
                     /locus_tag="AC16JC_12870"
     CDS             complement(108..662)
                     /locus_tag="AC16JC_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HIR5165635.1"
                     /translation="MALMDRAKQILLAPKEAWPVIEAEQTTIGALYTGYIIPLAAIGA
                     AASLLGSSIFSVRGFRMPFAWAFRHALSQFVGALVVVFVLSIVIDRLAPTFDARRNAI
                     QALKLATYSWTAYAVGGFFALIPPLAIPLLAILGLYSLYLLYLGLPVLMQAPTDKTVG
                     YLVVVTIVAVILFYLVSLVIGGLA"
     gene            822..3080
                     /locus_tag="AC16JC_12875"
     CDS             822..3080
                     /locus_tag="AC16JC_12875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020476000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5165636.1"
                     /translation="MRRIAFAVSLAVLWVPRAAPAQIPPAASDMLRRLFATRDFTGQS
                     FGGGAVHWMSGGAAFATLEPSALVPGSSDLVRYETATGTRSILITARQLIPSGAAEPL
                     EVEDYDFGPGDTLLLLFSNTRRVWRQNTRGDYWVLNSRSGTLRRMGGPGVPEASLMYA
                     KFSPSGDRVAYVLRGDIYVQRLADGTITRLTTDADSLHVNGMSDWVYEEEFGLRDGFR
                     WSPDGTRIAFWRFDMTGVRTFTLINDTDSLYPFTIPIQYPKTGTPNSAVRAGVVGADG
                     GPTTWIFLQDDLRDNYLPWMEWAGNNELLLQRMNRQQNTDRVMLADAVTGGTRTVLTE
                     QDGAWVDLVGNVTWLAGRTRFLWISERDGWRHVYAVPRQGGVPLLLTPGSFDVIDVAA
                     VDQTGGWLYYIASPDNATQRYLYRIRLSGGGRPERITPQDAPGTHSYTVSPDTRWAIH
                     TVSRFDTPPVTNLVRLPSHEVVRTLVDNAALRAAVDSLAPRPTEFFKVQVGGGVTLDG
                     WMIRPRDFDSTRTYPLLMEVYGEPAGQTVLDRWPGTSGLWHRMLADQGYVVASVDNRG
                     TPAPRGRAWRKIVYKQIGVLSSREQADAVRALTRSRSYLDSTRVAIWGWSGGGSSTLQ
                     AMFRFPEVYAVGMAVAPVPDQRLYDTIYQERYMGVPQENGDAYRLASPITFADGLRGR
                     LLLVHGSGDDNVHYQGSERLVNRLVSLGKQFDLMVYPNRSHCICEGRGTTLHIYTLLT
                     RYLLEHLPAGGR"
     gene            complement(3133..4524)
                     /locus_tag="AC16JC_12880"
     CDS             complement(3133..4524)
                     /locus_tag="AC16JC_12880"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5165637.1"
                     /translation="MTQTPSPALTRALSPLEYFAFGFGSMVGVGWLVVMDDWLARGGP
                     AGAMLGFALGGLLLLPVALTYGRLVRDIPDAGAEVAYTEGAFPPLASFGTGWIMVLAY
                     AIVCPWEAVAIGNLLARVFPALNSHALYAVAGKTIFAPRLAAGLALTALIAVVNVRGI
                     LPSGRFQAATTLGLLACFIVFVALGFARGSAGHLEPLFARPGTAGALGSVLLVAQIVP
                     YFMTGFESVAKGSEEAAPGFDPRGFGRAMTRAVLVGAAFYVLIIAAVSFVFPWRDLVA
                     GRLGTEAAFARAFGSAAIARLILFATFLSLLKVFNGNFVAATRLLYAMGRRHLIAPAV
                     ARIHARWGTPAVAIGLLTLFTVAGACLGDALLVPISEVGSLAAGVGWCAACLALIVRA
                     RRSGVRPSGTGGLRLAALGAIVSAAIVLMKVVPVVPGSFTGAEWIALGGWVVLGLALS
                     GGRWRSVEDGGRR"
     gene            complement(4609..4782)
                     /locus_tag="AC16JC_12885"
     CDS             complement(4609..4782)
                     /locus_tag="AC16JC_12885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165638.1"
                     /translation="MNRELWSCLILLAGRRLDKDGDLREPLAVRIRGAPFFDGQHRRT
                     TRKHDETDGGHGR"
     gene            complement(4800..5522)
                     /locus_tag="AC16JC_12890"
     CDS             complement(4800..5522)
                     /locus_tag="AC16JC_12890"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR5165639.1"
                     /translation="MRATLLALGPLVAFMSAGFSQGLPAQSRYSAGWADIAAVGAAGV
                     STLVPKLLVLPRGGPSCAPCNPAGLPGIDRWVVGANSSVARRASDVLLIGINGLAAGL
                     VAVERSPGQARGSLAVLANSVGWTAASTEWLKVLVHRKRPVLYTGAAAAAAPYRTNQQ
                     SFPSGHTSVAFAVATSYLAVAQREHLPHRARNATVLFGSATVIGALRMAGGRHFPTDV
                     IGGAFLGSGIGWLVAAVHPRTP"
     gene            complement(5537..>6784)
                     /gene="ppk1"
                     /locus_tag="AC16JC_12895"
     CDS             complement(5537..>6784)
                     /gene="ppk1"
                     /locus_tag="AC16JC_12895"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR5165640.1"
                     /translation="RIEVEQAMPQAIRDLLLRELQFDDAGEVSTLGPGDLYEATPYVD
                     LGALRELASLSRAELHYPPFRGRTPLPGDRSVFEALQHGDVLVHHPYDSFEPTVERFF
                     AEAADDPAVLAIKLTLYRAGGRSGIVDALLRAAVAGKEVFVFVELKARFDEERNVDWA
                     RRLEEAGIHVVYGLVKLKTHCKTALVVRREGDVVRRYVHIGTGNYNATTAALYTDLGL
                     LSADEELGADVNDLFNELSGSSRPPESPYRRLLVAPTHLLPRFLAMIDREIQHVRAGR
                     GGRIRAKINGLSDREIIVALYRAAQAGVEVALSVRGLCLLRPDVPGLSERIRVVSVLG
                     RFLEHARVFHFANGGAPEYYIGSADWRPRNLRRRVEVVAPVRDPASRRRLDEILTADL
                     EHPAAWELRSDGTYERRRPPPPR"
BASE COUNT         1125 a         2322 c         2407 g          930 t
ORIGIN      
        1 gcgcggggga cgcgggattt caccgaggcc gaccggatcc gggaggagct gcgcgcccgg
       61 ggcatcgaga tcgaggacac cccgggcggg acgaagtggc gacggagcta ggctagcccg
      121 ccgatcacca gggacacgag atagaacagg atgacggcca caatcgtgac gacgacgaga
      181 taccccaccg tcttgtccgt cggcgcctgc atgagcaccg gcagtccgag gtacagcagg
      241 tagaggctgt acaggccgag aatggcgagc agtggtatgg cgagcggggg aatgagggcg
      301 aagaatccgc cgacggcata cgcggtccag gaataggtcg ccagtttgag cgcctgaatc
      361 gcattgcgtc gagcatcgaa cgtcggcgcg agccggtcga tgacgatgga tagcacgaag
      421 acgaccacca gggcgccgac gaactgggag agcgcatggc ggaacgccca ggcgaacggc
      481 atccggaatc cgcggacgct gaagatggac gagccgagca gtgaggcggc ggcgccgatc
      541 gccgccagcg gaatgatgta accggtgtac aatgcgccga tcgtggtctg ttcggcctcg
      601 atcacgggcc atgcttcctt cggcgccaac aggatctgct tcgcccgatc cataagtgcc
      661 atgtggtgcc tccggctcgg gtgacgtgac gcgtgatgat gaaaaggcgt gcgctgcgct
      721 cccaccttgc gtcacccggc cccgttccgc aagtcgcacc cctttacgcc gccagccacg
      781 caggggcatt ttcccgattc gccttctcga ggagctgcgt gatgcgccgt atcgcgttcg
      841 ccgtgagcct cgccgtcctg tgggtgccgc gcgcggcgcc ggcgcagatt ccccccgccg
      901 cgagcgacat gctgcggcgg ctgttcgcga cgcgtgactt caccgggcag tcgttcgggg
      961 gcggggcggt gcactggatg agcggcgggg cggcgttcgc cacgctcgag ccgtcggcgc
     1021 tcgttcccgg ctcttccgat ctcgtgcgtt atgagaccgc caccggcacc cgcagcatcc
     1081 tgatcaccgc gcgccagctc atcccgtcgg gcgccgccga gccgctcgag gtcgaggact
     1141 acgatttcgg tcccggtgac acgctgttgc tcctcttcag caacacgcgc cgcgtgtggc
     1201 ggcagaacac gcggggcgac tactgggtcc tgaactccag gagcgggacc ctccgccgga
     1261 tgggggggcc gggcgtgccc gaggcctcgc tcatgtacgc gaagttctct ccctcgggcg
     1321 atcgcgtcgc ctacgtgctc cgcggcgaca tctacgtcca gcggctggcg gacggcacca
     1381 tcacgcggct cacgaccgac gcggactcgc tccacgtcaa cgggatgagc gactgggtgt
     1441 acgaagagga gttcggcctg cgcgacggct tccgctggtc ccccgacggc accaggatcg
     1501 cgttctggcg cttcgacatg accggggtgc gcacgttcac gttgatcaac gacaccgatt
     1561 cgctctaccc gttcacgatc ccgatccagt atcccaagac gggtaccccg aattcggcgg
     1621 tgcgcgccgg cgtcgtcggc gccgacggcg gcccgaccac ctggatcttt ctccaggacg
     1681 acctgcgcga caactacctg ccctggatgg agtgggcggg caacaacgag ctgctgctgc
     1741 agcggatgaa ccggcagcag aacaccgacc gggtgatgtt ggcggacgcg gtcaccggag
     1801 gcacccgcac cgtgctcacc gagcaggacg gcgcgtgggt ggacctggtg ggcaacgtca
     1861 cctggctcgc ggggcgcacc cggtttctgt ggatctccga gcgcgacggg tggcgccacg
     1921 tgtatgcggt gccgcgccag ggcggggtgc cgctgctcct gacgcccggc tccttcgacg
     1981 tgatcgacgt cgcggcggtg gaccagaccg gcggctggct ctactacatc gcttcgccgg
     2041 acaacgcgac gcagcggtat ctctaccgca ttcgcctcag cggaggcggg cgcccggagc
     2101 ggatcacgcc gcaggatgcc ccgggcacgc acagctacac cgtctctccg gatacccggt
     2161 gggcgattca caccgtgtcc cgcttcgata cgcctcccgt cacgaatctc gttcgcctgc
     2221 cgtcgcacga ggtggtccgc acgctggtgg acaacgccgc gctgcgcgcg gcggtggact
     2281 cgctggcgcc ccgcccgacg gagttcttca aggtgcaggt cggcggcggc gtcacgctcg
     2341 acggctggat gatccggccg cgcgatttcg attcgacccg gacctatccc ctgctcatgg
     2401 aggtatacgg cgagccggcc gggcaaacgg tgctcgatcg ctggccaggg acgtccggcc
     2461 tgtggcaccg gatgttggcg gaccagggct acgtcgtcgc cagcgtcgac aaccgtggca
     2521 ctccggcccc gcggggccgt gcctggcgca agatcgtgta caaacagatc ggcgtgctgt
     2581 cgagccgcga gcaggcggac gccgtgcgcg ccctcacccg cagccggtcc tacctcgatt
     2641 ccacccgggt ggcgatttgg ggctggagcg gcggcgggtc gagcaccctg caggcgatgt
     2701 tccggttccc cgaggtgtac gccgtgggga tggccgtggc gccggtcccc gaccagcggt
     2761 tgtacgacac gatctaccag gaacgctaca tgggcgtgcc ccaggagaac ggggacgcct
     2821 atcggctcgc gtcgcccatc acgttcgccg acgggctgcg cggccggctg ctcctggtgc
     2881 acggctcggg agacgacaac gtccactacc agggatcgga gcggctggtg aaccggctgg
     2941 tctcactggg gaagcagttc gacctcatgg tgtacccgaa tcgctcccac tgcatctgcg
     3001 aggggcgcgg cacgaccctg cacatctaca cgttgttgac tcggtacttg ctggagcact
     3061 tgccggcggg ggggcgctga actgagggac gggggaagac ggtggaggac ggtggaagtg
     3121 ggtggaggtc cttcaccgtc ttccaccgtc ctccaccgac ctccaccgtc ctcctgacag
     3181 tgccaggccc agcacaaccc accctcccag cgcaatccat tcggctccag taaagcttcc
     3241 gggaaccacc ggcaccactt tcatcagaac gatggctgcg ctcacgatgg cgccgagggc
     3301 ggcgaggcgc agcccgccgg tccccgaagg ccgcacgccg ctgcgccgcg cgcgcacgat
     3361 cagggcgagg caggcggcgc accagccgac gcccgccgcg agcgagccca cttcgctgat
     3421 cggaacgagc agcgcgtcgc cgaggcacgc cccggcgacc gtgaacagcg tgaggagtcc
     3481 gatcgccacg gccggcgtgc cccagcgcgc gtggatgcgc gcgaccgccg gggcgatgag
     3541 gtgccgccgc cccatcgcat agagcaaccg tgtcgcagcc acgaaattcc cgttgaagac
     3601 cttgaggagc gagagaaatg tcgcgaacag gatcagccgc gcgatggcgg ccgacccgaa
     3661 ggcgcgcgcg aacgccgcct cggtgccgag ccgcccggcg acgagatcgc gccaggggaa
     3721 gacgaaggag acggcggcga tgatgagcac gtagaacgcc gcccccacga gtaccgcccg
     3781 cgtcatcgcc cgtccgaacc cgcgcggatc gaaacccggc gccgcctcct ccgatccctt
     3841 ggcgaccgac tcgaacccgg tcatgaagta cggcacgatc tgcgcgacga gcagcaccga
     3901 tcccagcgcg ccggccgtcc cgggccgcgc gaacagcggc tcgagatgcc ctgccgatcc
     3961 gcgcgcgaaa ccgagggcga cgaacacgat gaagcaggcg agcaggccga gcgtcgtggc
     4021 ggcctggaac cggccgctgg ggaggatgcc gcgcacgttg acgacggcga tgagcgcggt
     4081 gagggcgagc ccggcggcga gccggggggc gaagatggtt tttccggcga cggcgtacag
     4141 ggcgtgactg ttcagcgccg gaaacacccg ggccaggagg ttgccgatcg ccacggcctc
     4201 ccaggggcag acgatggcat aggccaggac catgatccac ccggtgccga agctggcgag
     4261 cggcggaaac gcgccttcgg tataggcgac ttcggcgccg gcgtcgggaa tgtcccggac
     4321 caggcgcccg taggtcagcg ccaccggtag cagcagcagt ccaccgaggg cgaagcccag
     4381 catcgcgccg gcggggccgc cacgggccag ccagtcgtcc atgacgacga gccacccgac
     4441 ccccaccatg ctgccgaacc cgaaggcgaa gtactcgagc ggtgagagcg cgcgcgtgag
     4501 cgccggcgac ggggtctggg tcaacggggg gcgaatgcgt cggtcatggc tgcaccgcgt
     4561 gcacggcgta gaccgggtgc agctcccaca gggcacggag cgacgagttc agcgtccgtg
     4621 cccgccgtcg gtttcgtcgt gcttgcgcgt cgtgcggcgg tgctgcccgt cgaagaacgg
     4681 cgccccccgg attcgcacgg cgagcggctc gcgcagatcg ccgtccttgt cgaggcgccg
     4741 gccggccagc agtatcaggc acgaccacag ctccctgttc acacgccggt cccccggaat
     4801 caaggtgttc tcggatgcac ggcagccacc aaccagccga tgccactgcc caggaacgcg
     4861 cccccgatga cgtcggtcgg gaagtgcctg ccgccggcca tccgcagcgc accgatgacg
     4921 gtagcgctcc cgaacaggac ggtggcattg cgcgcgcgat gcgggaggtg ctcccgctgc
     4981 gcgaccgcga ggtacgacgt cgcgaccgcg aacgccacgg aggtgtggcc ggaagggaag
     5041 gattgctggt tggtgcggta cggtgcggcg gccgcggcgg cgccggtata gaggaccggc
     5101 cgcttgcggt ggacgagcac cttgagccat tcggtcgacg ccgcggtcca gccgacggag
     5161 ttcgcgagca ccgcgaggct cccacgcgcc tgcccgggcg agcgctcgac ggcaaccagc
     5221 cccgccgcca ggccgttgat cccgatcagc aagacgtcgc tggcacggcg cgcgaccgac
     5281 gaattcgcgc ccaccaccca ccggtcgatg ccgggcagtc cggccgggtt gcacggggca
     5341 caggagggac caccgcgggg caggacgagc agcttcggca cgagcgtcga cactcccgcc
     5401 gcgccgacgg cggcgatgtc cgcccagccg gcgctgtagc gtgactgggc gggcaggccc
     5461 tgggagaagc cggccgacat gaaggcgacc agcggaccca gggcgagcag ggtcgcacgc
     5521 acgcacgcgc tccgggtcaa cggggggggg gaggccggcg ccgttcgtag gtgccgtcgg
     5581 aacggagctc ccacgccgct ggatgctcga gatcggcggt caggatctcg tcgaggcgtc
     5641 ggcggcttgc ggggtcgcgc accggggcca cgacctcgac gcgacgtcgc aggttccggg
     5701 gacgccaatc ggccgagccg atgtagtact ccggagcgcc cccattcgcg aagtggaaga
     5761 cccgtgcgtg ctcgagaaac cggccgagca cgctgaccac ccggatgcgc tcggacaacc
     5821 ccggcacgtc cggccggagg agacagaggc cgcgcacgga cagcgcgacc tcgacgccgg
     5881 cctgcgctgc ccggtacaac gcgacgatga tctcccggtc cgagagaccg ttgatcttcg
     5941 cacggatccg gccgcctcgt cccgcgcgga cgtgctggat ttcgcgatcg atcatcgcga
     6001 ggaaccgggg caggagatgc gtcggggcga ccaggagccg tcggtacggc gactccggcg
     6061 gccgcgacga gcccgagagc tcgttgaaca ggtcgttcac atcggcgccg agctcttcgt
     6121 ccgccgacag cagccccagg tcggtataca gggcggccgt cgtggcgttg tagttgccgg
     6181 tgccgatgtg cacgtaccgc cggacgacat cgccctcgcg gcgcaccacg agggccgtct
     6241 tgcagtgagt tttcagtttc accagaccgt agacgacatg gatgccggcc tcctccaacc
     6301 ggcgggccca gtcgacgttg cgttcctcgt cgaagcgtgc tttgagctcg acgaagacga
     6361 agacctcctt gcccgctacg gcggcgcgga gcagcgcatc gacgatgccc gagcggccac
     6421 cggcgcgata cagcgtgagc ttgatcgcca ggaccgcggg atcgtcggcc gcctccgcga
     6481 agaagcgctc gaccgtcggc tcgaacgagt cgtaggggtg gtgcacgagc acgtcgccgt
     6541 gctgcagcgc ctcgaacacg gaccgatccc cgggcagggg tgtccgccca cgaaacggcg
     6601 gatagtgcag ctcggcgcgc gacaacgaag cgagctctcg aagggcgccg aggtcgacgt
     6661 acggcgtcgc ctcgtacagg tcgccggggc cgagcgtgct gacctcaccg gcgtcgtcga
     6721 actgcagctc gcgcagcagc aggtcgcgga tggcctgggg catggcctgc tccacttcga
     6781 tgcg
//
LOCUS       DBNGZW010000543         1989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_188037, whole genome shotgun sequence.
ACCESSION   DBNGZW010000543 DBNGZW010000000
VERSION     DBNGZW010000543.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_188037"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..910
                     /locus_tag="AC16JC_12900"
     CDS             170..910
                     /locus_tag="AC16JC_12900"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HIR5165641.1"
                     /translation="MSPPAARCFVLADAHLGQVPANVEAAFHAFLETVPQAGDELLVA
                     GDLFDFWFEYGRVVPRRHVVTAMKLGDLVRRGIPVTFLGGNHDRWGGDFLRQDLGIAY
                     HAGAAEMDLAGRRALAVHGDGLTEQHWGGTLTHWLLRQPLTIAVFRSLHPTIGFWIAD
                     RLSGTLADATKDRAVLDRAEAAQASYAKALLERRPELSLVVMAHTHRARLELLPDGRA
                     YLNPGAFLDGGRYAVVTRETVELKSFGV"
     gene            complement(907..1704)
                     /gene="murI"
                     /locus_tag="AC16JC_12905"
     CDS             complement(907..1704)
                     /gene="murI"
                     /locus_tag="AC16JC_12905"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015351865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR5165642.1"
                     /translation="MNSAPLGVFDSGIGGLTVAHALFECLPHESVIYFGDTARVPYGP
                     KSPETVRRYSGEILDFLVRRGVKMVVVACNTSTAHALDFLRARSPVPVQGVIEPGARA
                     AAAATRAGRIGVIGTAGTIASGAYERAIKAVRPGVEVVSRACPLFVPLVEEGWFDHPA
                     TELVAREYLKPLKEQGVDVLVLGCTHYPLLKPLLSRVMGPDVTLIDSAAETARTVRHE
                     LEGRELLAPAGSLPAHHFVVSDDVALFRRVGARFLGEKLQDVELAPL"
     gene            complement(1708..>1989)
                     /locus_tag="AC16JC_12910"
     CDS             complement(1708..>1989)
                     /locus_tag="AC16JC_12910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165643.1"
                     /translation="TGRKVTGALFTVWEGTTMFMTLKASQEASYLRASGSPNLDSKHQ
                     EVQDWLVLWGFNHLFSGAEAFVAAHLRDFPRDLKIRAVPGGVGLRFSLP"
BASE COUNT          299 a          703 c          682 g          305 t
ORIGIN      
        1 gccgatgggc tccccgcctc ccggcacacc ggtcgccctg gagtgcgggc gccggattct
       61 ggactcgccg cgcgtcacgg tcatcgtgcc ggagaatgcg tggcctatga gcaaaagtca
      121 aatgggacac tggcttgcga ccccggaccc cggttcctag cttggggccg tgtctccccc
      181 cgccgcgcgc tgcttcgtgc tggccgacgc ccacctgggc caggttcccg cgaacgtcga
      241 agccgcattc cacgccttcc tcgagacggt gccccaggcc ggggacgagc tgctggtggc
      301 cggggacctc ttcgacttct ggttcgagta cggccgggtg gttccgcgcc gccatgtggt
      361 gacggcgatg aagctcggcg atctcgtccg ccgcgggatc ccggtgacct ttctcggcgg
      421 gaatcacgat cgctggggtg gagattttct ccggcaggac ctggggatcg cctaccacgc
      481 gggcgcggcg gagatggacc tcgccggacg gcgcgcgctc gccgtccacg gcgacggcct
      541 caccgagcag cattggggcg gaacgctcac gcactggctg ttgcgccagc cgctcaccat
      601 cgcggtgttc cgctccctcc atcccacgat cggcttctgg atcgccgaca ggctctccgg
      661 caccctggcc gacgcgacga aggacagggc cgtgctcgat cgcgccgaag ccgctcaggc
      721 gtcctatgcg aaggcgctgc tcgagcgccg cccggagctg tcgctcgtgg tcatggccca
      781 tacgcaccgt gcgcgcctcg agctgcttcc ggacggacgc gcctacctca atccgggcgc
      841 gtttctggat ggtgggcggt acgccgtggt gacgcgcgag accgtggagc tgaagagttt
      901 cggcgtctag agcggcgcca gctccacatc ctgcaacttt tccccgagga accgggcgcc
      961 gacgcgacgg aagagcgcca catcgtcgga gacgacgaag tggtgcgccg gcagcgagcc
     1021 ggcaggcgcc aggagctcgc gcccttcgag ctcgtgccgg acggtccggg ccgtttccgc
     1081 ggcgctgtcg atcagcgtca cgtccggacc catcacccgc gacagcagcg gcttgagcag
     1141 cggatagtgg gtgcagccca gcacgaggac atccacgccc tgctccttga gcggcttcag
     1201 gtattcgcgc gcgaccagct cggtcgccgg gtggtcgaac catccctcct cgacgagcgg
     1261 cacgaagagc gggcacgccc gggacacgac ctccaccccc ggacgaacgg ccttgatggc
     1321 gcgctcgtac gcgccgctgg cgatcgtgcc agcggtgccg atcacgccga tgcgcccggc
     1381 acgggtggcg gccgccgcgg cgcgcgcccc cggctcgatc accccctgca ccggcaccgg
     1441 cgagcgggcg cgcaggaagt cgagggcgtg cgcggtgctc gtgttgcagg cgacgaccac
     1501 catcttcacg ccgcgccgca ccaagaagtc gaggatctcc ccgctgtagc gccgcaccgt
     1561 ctccggtgac ttgggaccgt aggggacgcg cgcggtgtcg ccgaaataga tgacggattc
     1621 gtgcggcagg cactcgaaca gcgcgtgcgc gacggtgagt ccgccgatgc ccgagtcgaa
     1681 gacgcccagt ggggccgagt tcatgcctta cggcagggag aaccgcagcc cgacgccgcc
     1741 gggcactgcc cggatcttca ggtccctggg aaaatcgcgc aggtgcgccg cgacgaaggc
     1801 ttcggcgccg gaaaacaggt ggttgaatcc ccacaggacg agccagtcct gcacttcctg
     1861 atgcttggag tccaggttgg gcgagccgga cgccctgagg tagctcgcct cctggctcgc
     1921 cttgagcgtc atgaacatcg tcgttccttc ccagacggtg aacaacgccc cggtgacctt
     1981 gcgccccgt
//
LOCUS       DBNGZW010000544        21676 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_663276, whole genome shotgun sequence.
ACCESSION   DBNGZW010000544 DBNGZW010000000
VERSION     DBNGZW010000544.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 21676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 21676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21676
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_663276"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="AC16JC_12915"
     CDS             <1..1040
                     /locus_tag="AC16JC_12915"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR5165644.1"
                     /translation="TTVKVTEQEVQDAVEQTVRNVRGRFTSETEFQQQLRLAAFGGVE
                     EWRRWLTENQRREILRTRLLENLRQREKIKPIPPSDAQLRAYWEENKGQAQQRPANVS
                     FRQIVILPRPDSAARARARATAESLVVVLRGGADFAELARRLSADSTTRDSGGTLGWF
                     RRGTMVKAFEETAFRLRPGEISDVVETDFGFHIIQVQRIQPAEVLARHILIAPEISAV
                     QVESARRLADSVRAALARGASFDSLAQRYGDQNVPKLAEAVAIAQLGPEYQRLFAADS
                     TKGLKPVLELGAGGARPQFVVLEVTGRQAEGEVTYDDVRDQIRTQLAQELGVRHYVDQ
                     LRRQTFIDIRL"
     gene            1043..1981
                     /gene="pdxA"
                     /locus_tag="AC16JC_12920"
     CDS             1043..1981
                     /gene="pdxA"
                     /locus_tag="AC16JC_12920"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010661663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HIR5165645.1"
                     /translation="MAAARRPRLAVTLGDPRGIGPEVVTRALAGGPLGADVVLVGPDD
                     LLNGIAARSRIGVGNWGRGTGDEERAGEERARAAGQLAVRAIERAVWLARAGEVDAVV
                     TGPVEKRALHLAGYQFPGVTELLSRLAGGIPTAMMLAAGPLRVVLVTTHIPLREVPQR
                     LTTEGIVAVGRLTAAALERWWGLATPRLVLCALNPHAGEGGLFGDEDDRILRPAAQAL
                     GAAGPLPADTVFVKALRGMFDAVLAPYHDVGMTAVKVAGFGKGVNVTLGLPFPRTSPD
                     HGTAFDIAGKGVADPGSMRAALELATELAQHGRVRD"
     gene            complement(1991..3322)
                     /locus_tag="AC16JC_12925"
     CDS             complement(1991..3322)
                     /locus_tag="AC16JC_12925"
                     /inference="COORDINATES: protein motif:HMM:NF013741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HIR5165646.1"
                     /translation="MPAPAAESLGLRLAAVAILILVNAFFVAAEFSLVAARRTRIEAM
                     VRRGDRKAKTVLVALHDMNRQLSAAQLGITVASILLGYVAEDTVAHLFRGWFSALPAA
                     LSFLTRGGVASVVAVSIVSFLHVVFGEQAPKAWAITYPEGTSRWIAAPLIFFSLITRP
                     FTGLLNWSANRIVRLFGIKGGTAEHERVHSPEEIMMLVEQSRKTGRLAPQDARMIEGV
                     FEFSEKNARDVMTPRTQVVALQSDLTLVQAADRVAAAGRSRYPVYRESLDDIIGVVHA
                     KAILAGLRADPAQRLEAIMRPTLFVPGTREVEDVLADMKRQKVHMAIVLDEYGGTAGL
                     VTMEDLLEEIVGQIYDEYDRPETSVAAGSATAPAPVIAGGTEVADVNRTYGLQIDDTD
                     YTTIGGVLFGQLGRLPKVGDRLEIAGASFEVLEMDGRRVGKVRVTQPRPAA"
     gene            3395..4291
                     /locus_tag="AC16JC_12930"
     CDS             3395..4291
                     /locus_tag="AC16JC_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015231621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR5165647.1"
                     /translation="MTQTLPLDVQCAHRVPERSESARRLAWVLGLTATVMVAEAVGGW
                     LAHSLALLADAGHMLADVVALGLALMVLRMAQRPATPERTYGLVRLEILAALVNGAAL
                     IAIALGIGIEAVRRLQVPPAVHGGLLLGVASVGLLANLAGVTILHRGHHHSLNQRGAY
                     LHVMSDLLGSVGAVLAGVLVVATGWRLADPIVSLGIGVLVLSGAWRLVRESADVLLEA
                     APAHIALGTVHERLASLPGVSSVHDLHVWTLTSGVIAMSGHLVVRNPEQNQQILEAAQ
                     ARLEQLGIHHVTVQMERDVTCD"
     gene            4361..6193
                     /gene="typA"
                     /locus_tag="AC16JC_12935"
     CDS             4361..6193
                     /gene="typA"
                     /locus_tag="AC16JC_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HIR5165648.1"
                     /translation="MQIRNIAIIAHVDHGKTTLVSQMLRQARALRHGATGFLDTGIEA
                     ERGITILSKNTAVRWGETKINIVDTPGHADFGGEVERILRMVDGFLLLVDAAEGPMPQ
                     TRFVAGKALALGLKPIVVINKIDRQDAEPLRVHDEVLQLFLDLEATPEQFNCPFLYAS
                     SRQGIATPDLGTPGTSLKPLFETILATVPPPSGDPDGPLQMLISTLDYSSYLGRIGIG
                     RIERGRVRVGETVALLPLGMPGPVGDGTYEQARVVKLFGFEGLERVELEEAAAGEIVA
                     LAGLSGVEIGKTVTAPDHLERLAGIAVEEPTISVDFRVNDAPFAGREGKYVTGRQLRD
                     RLYKELERNVALRVEDTDDPQTFTVSGRGELHLGILMETMRREGFEFQVSRPRIIPRE
                     GPNGEQLEPYEELLIDCPEGYLGVVMEKLGPRRATMKDMRNPGVGSVRLRFAIPARGL
                     LGYRSEFLTDTRGTGTMHHRFLEYGPWAGPLSGRSRGVMVADREGVAVAYALFNLQER
                     GTMFVKPGDPVYEGMIVGEHVRPGDLDVNATKEKKLTNMRTTASDEMVILEPPRQITL
                     ELALEYIEDDELIEVTPSSVRLRKRELQATARRKLARSLRAMDE"
     gene            6223..6471
                     /locus_tag="AC16JC_12940"
     CDS             6223..6471
                     /locus_tag="AC16JC_12940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165649.1"
                     /translation="MLAVATSMGWSLKRRSDDIDEEFEDFEEDKELEEDFDDELDEDE
                     LDDEEMEDLDDYDDLDDEFDDEDEDGFRPKKGPKREWD"
     gene            6749..7372
                     /locus_tag="AC16JC_12945"
     CDS             6749..7372
                     /locus_tag="AC16JC_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HIR5165650.1"
                     /translation="MTRKLAGPLVGALGVLAVAVLGIQVAFAHCDGLDGPVVKAAQSA
                     LQTGNVNLVLVWVQEKDEPEIRAAFQQALVVRKQSPEAQALADRSFFETLVRIHRAGE
                     GAPYTGLKPAGRDLGPAIPAADKALETGAVGPLQDLLANAVQHGLKERFEALRTLRGY
                     DPNDVAAGRRYVQAYVEYVHYVERLHEAATAAAEGHYAEARTGEPHR"
     gene            7606..8754
                     /locus_tag="AC16JC_12950"
     CDS             7606..8754
                     /locus_tag="AC16JC_12950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR5165651.1"
                     /translation="MLLTTGAVFSGCGKTRASPGDSAVNLGSAADSVAEGQGIFRFDT
                     FGDEQFWTDTLHMNQVVEQSVDPTTALAVGLKVDADVLPPGILQQVDLKSPATTVALL
                     KMNAVVGIQATVDSNNHITRLGITCALCHSNVDNSVMAGIGHRLDGWPNHDLNVGAIV
                     ALSPVLSAAQKAVYQSWGPGKYDPRYNLDGKNGALVIPPAYGLAQVKNETYTAEGPIS
                     YWNAYVAVTQMHGHGNFSDARLGIRVTQSPDMVGPKLAALRAYQHSLAAPPPPAGSFD
                     TAGAQRGRALFARMCTSCHVGVTGTDNNDGKLHAPAETGVDGAYAARTATKAYRTTPL
                     RALWQHAPYFHDGSAATLPDVVTHYNSVRKLGLTAAQQHDLIEYLKSF"
     gene            9061..9134
                     /locus_tag="AC16JC_12955"
     tRNA            9061..9134
                     /locus_tag="AC16JC_12955"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9095..9097,aa:Ile,seq:gat)
     gene            complement(9229..9951)
                     /locus_tag="AC16JC_12960"
     CDS             complement(9229..9951)
                     /locus_tag="AC16JC_12960"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5165652.1"
                     /translation="MTFEESPDQVQAGIDHVLDDVIPALETSPGLSGLWLVDREHGKR
                     ISIMVWESEAHASAAMARVQDRVARSKHQRPTPTKVERFEIYARVPAASQPAESVVRS
                     VMAAIEAGQFAQARSLLHDDFTFSGPVPQPIGPDAWIGVHRALYAAMPDLRFNASGFQ
                     TTGSGVHFGVALTMTHTGTLDLAPLGVRAFAPTGKSVKLVPESATVMVRDGKLASWAN
                     VTPPDGGLVGIARQIGASIGGQ"
     gene            10035..10577
                     /locus_tag="AC16JC_12965"
     CDS             10035..10577
                     /locus_tag="AC16JC_12965"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR5165653.1"
                     /translation="MAKRARARDAPQFLDDDEVAAWHGLLQTSGRVLRELDQRLQREH
                     TISVGEFDVLITLFNAPDQRLRMAELARSVSLSPAGLTHLVARLERERLVRRATDPED
                     RRGSYTVLTAKGLARLNAARPVHNEVIRHLFLNRITTDDRRRLAVIWRRAQGSAPRAP
                     ASSGSASAGAGGLGGQAAPR"
     gene            10663..11889
                     /locus_tag="AC16JC_12970"
     CDS             10663..11889
                     /locus_tag="AC16JC_12970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020640173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5165654.1"
                     /translation="MRVLLSTIGSRGDVQPLVALALQLKALGQEVRLCVPPDFRDWIE
                     SLGMPVTPIGPELRSTGKASPSAALPTPEQRRQMTEATVAAQFATITMAAQGCDLIVG
                     ATALQIAAPSVAERMSIPYVFAAYCPNVLPSPHHAPPVYSLQGDAPTAAPADYSELWA
                     RNAERFNTSFGAALNAHRAALGLAPVGDVRSHIFTSRPWLAADPTLAPWPDPADDAVF
                     QTGAWILPDERPLSRELEAFLEAGEPPVYFGFGSVRAPEGLSRVMIESARALGRRAIV
                     SRGWADLALVDDEPDCLAIGEVNQQALFKRVAAVVHHGGSGTTTVAARAAAPQVVIPQ
                     HYDQHYWAQRVHHLGIGTAHAPGTPTPDSLTSALEHTLQPDVATRARSIATVVRSDGA
                     HAAALYVMVADPRNSL"
     gene            12170..12844
                     /locus_tag="AC16JC_12975"
     CDS             12170..12844
                     /locus_tag="AC16JC_12975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5165655.1"
                     /translation="MVAAVPMQHPPRVSELVRRRLKELERTPLELAEALQVPESYVAD
                     LLSGRRRPPAPGRTDIYDRMTKFLRMPRNELSTCARAELAADPSRQRPPDQKVKKLLL
                     ELCEPTRARTLSRRLADKKGNPALEILIAQRLLEVAQGFVRRQLDDEVGIRVAATREG
                     CTHSEMRLRLIDFLDATADSLTVTDHEVYVRPRIATWDIDLETHAMKIVLRSHEPEPR
                     QRRWTR"
     gene            12895..14568
                     /locus_tag="AC16JC_12980"
     CDS             12895..14568
                     /locus_tag="AC16JC_12980"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018472417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR5165656.1"
                     /translation="MRCWLLCACALLFPPASGAAQEPTPRTFTHADSLRGADTPQRAW
                     WDVTFYDLHVAVHPADSTIAGYNGITYRVLRPAREMQIDLQEPLTVDSMVQDGRRLSF
                     RRDGNAFFATLGTPQRAGERRTITVYYHGKPRAAVRPPWDGGLIWAQDSAGNAWVATA
                     NEGLGASVWWPNKDYLADEPDSQRVAITMPDPMFDVSNGRLRGVTPHPDSTTTYEWFV
                     TQPINNYDIAINAGGYAHFATIYSGEAGPLTMDFWPLAYHRAAAERQFTQAESMMRCF
                     ESWFGPYPWYEDGYKLVETPHLGMEHQTAVAYGNHYGNGYLGRDLSHTGLGLLWDFIV
                     VHESAHEWFGNSITMRDQADMWVHESFANYSENLYTECLAGKQAGAQYVIGTRQNIRN
                     DRPIVGQYGVNDQGSGDMYYKGGNMLHTIRQIVGDDAKWRSILRGLNSTFRHQTVTGQ
                     QVQDYISRESGVDLRKVFEQYLTTTLVPVFEYSIVDSTLAYRWTQVIAGFDMPVKATV
                     TDSGFTLLRPTETWQRTAIHLKDPVTFHLDQNFYVLTRHDVAPPPQAGR"
     gene            14575..15081
                     /locus_tag="AC16JC_12985"
     CDS             14575..15081
                     /locus_tag="AC16JC_12985"
                     /inference="COORDINATES: protein motif:HMM:NF016213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HIR5165657.1"
                     /translation="MTGILPGLLLAVCCVAGLVLVALGLPGLWVMVLGVIAYAWMTGF
                     HTIGAVTISVVLGLAVVGEIVEWWVGFWFAERYGGSRRAGWGALLGGIVGAVVGVPVP
                     VIGSVIGAFVGAFAGAALFEYTRQLSPGVALRAGWGAVLGRAAASALKIALGLAIAVV
                     AAFEAIRG"
     gene            complement(15078..15935)
                     /locus_tag="AC16JC_12990"
     CDS             complement(15078..15935)
                     /locus_tag="AC16JC_12990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5165658.1"
                     /translation="MRLSVAALLLLVACGNGTDPRIESDALLVGAGDIASCVSTNDEA
                     TARLLDTLPGAVFTAGDNDYSDGLVAPAYGVCFDSTWGRHRARLHPAPGDDDRRGGNL
                     TLDDYYAYFGAAAHPPSGAYSYDLGAWHIVVLNATPMVDTAQLSWLRADLTAHPTRCA
                     LAIAHRPRFSSGNTGSSPGMGAVFQTLYDHGVELLISGNDHDYERFAPQAPDQTLDSA
                     AGVVQLVVGTGGKSHGRINLPLALNSVAQNDDSYGVLRLVLHPTSYEWLFVPVPGRTF
                     SDAGQLPCH"
     gene            16004..16990
                     /locus_tag="AC16JC_12995"
     CDS             16004..16990
                     /locus_tag="AC16JC_12995"
                     /EC_number="1.6.5.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HIR5165659.1"
                     /translation="MRFLHVVERDGRYAAELREAPDPVPAAGEVLVRVAASGVNRADL
                     SQIAGRYAPPPGESSILGLEISGTVVETGAPVCALLGGGGHAELVAVPTGQILPVPPA
                     LDLAMAAALPEAYLTAFVNLVVEAGLRAGDRVLIRAGASGVGLAAIQVARYVGAQVAA
                     TTRSPDKRAALEGVGADLAIVTTADGGTAEIERHWGRDAVDVVLDPIGGSSVAGDLQL
                     LAPGGRIVVIAAMDGTSAEIDLRLLMKKRARLVGSTLRARSRAEKARLTARFRDECFP
                     GFEAGRLGVNVEAVYGPEDAAQAYQRMRENRNTGKLLIRWPASCESSERPPS"
     gene            17016..18488
                     /locus_tag="AC16JC_13000"
     CDS             17016..18488
                     /locus_tag="AC16JC_13000"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR5165660.1"
                     /translation="MTVAPSRRAAPGRSSGPGPSLRAVLDAALDAVILMDDDGVIRFW
                     NRQAEALLGWTAPEAVGRSFSSLIDPERPAGRRKHGVAGRTADERRQAARRREVTARH
                     RDGHVVPVELGITPIHDRTSRFQGAWLRDISERRRREAVERLQSCILENVHDSVILVD
                     LQGRVQYWNQGAEALYGYKASEMLGRSIGAILPEGEAVEPDLEHVLATGDTSEEARRR
                     HKDGTPLWVESRRSVMRDAAGDVLGVLGVVKDVTQRHRVAEELERSRAQLRNLAVRLR
                     RAREEERTSIARQIHDEMGQVLTALRMDVAWLEARIPRDQPSLLDKCATMARLIEGMI
                     GQVRTLAAQLRPGVLDELGLAAAIEWETQEFARRTGIACTLHLRAELAGVDPDRAIDL
                     FRILQEALINVARHARAQQVGVRVSVTRKGREREVVLHVEDDGRGIRPEETADARSLG
                     LLGMRERALLWNGGVEVRPRAEGGTRVTVRLPLPATGLSA"
     gene            18485..19117
                     /locus_tag="AC16JC_13005"
     CDS             18485..19117
                     /locus_tag="AC16JC_13005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5165661.1"
                     /translation="MNRPIRVAIADDHPLVRAGIKQVIADDETLTVVGEAADGDATVT
                     MVRSTLPDVLVLDLSMPGAPFPGLLRVLRAAFPTLRILVVTMHTEEQFAARALREGVA
                     GYVTKDRPPDDIVAAVRQVAAGGRYITAALADSVTDAANAELPHERASQREYEVLCMI
                     GCGKTVKQIAIELGLSPKTISTHRARLLRKMQLKSSAELIRYAVQHGLSS"
     gene            complement(19119..20189)
                     /locus_tag="AC16JC_13010"
     CDS             complement(19119..20189)
                     /locus_tag="AC16JC_13010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165662.1"
                     /translation="MHFPVVRVAGIAVFVSFLTAPARATAAIGERSAGLPPGVRVDST
                     RHTVVILAGPFHVASAMPMMPDMEMEHMAGDDSLVKRFDWPMTTLLQGLRLDLSDGAG
                     RPLPRGLLHHLNLVNFDRRALVYPLVERMMGFGQETEDVSVPSSIGLPMTAGQRIGVF
                     VMWDNKTGRDLDDVYVQLTFRWAPGNLVPRPTEVMPFTIDAHLVPAGHNTFVVPPGGT
                     TQRFDFTLPLDGHLVAASGHLHDHGVRVSLLDRTTGRTVVSVRSRRDSTGHVVGMSRA
                     LLALRGAGPHLRADHRYRLEVEYENPSTDTLTGMMGLMVGLFAPDDPARWPAIDPNDP
                     DYRKDLADILGSAVAGVTEARR"
     gene            complement(20257..>21676)
                     /locus_tag="AC16JC_13015"
     CDS             complement(20257..>21676)
                     /locus_tag="AC16JC_13015"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HIR5165663.1"
                     /translation="KLLACDPTVRSPRLVGQIASTGSFHDMDNSFTLIGFIVLVGLLV
                     LSSVVRARRREAAALAATRRAGLSPTGPRAQHPHIDVSHCIGCGTCVDACPEDVLAVI
                     AGKAAIISAHKCIGHGLCAEACPVGAIEIVMASPSIAADLPRLSPQLETNVPGLFVVG
                     ELGGLALIKNAVNQGRDCIDTIAQRLTRSGHAPAGVRDVCIVGAGPGGISASLRAVER
                     KLSYVTLEQDEFGGTVAKYPRQKVVMTSPVEFPVYGKFKKLQLSKEELLKFWNKIHKE
                     AGLAVRTGEKVDTITREEDGFFTIATPRGRYRSRAVILAIGRRGSPRKLGVPGEELPK
                     VMYSLLDAGAYTGVRILVVGGGDSAVETAMGLAHQRGNSVTLSYRQHEFSRIKERNAQ
                     RLKEFVKSGRLTVVFDSQPVEIHERVVVLEVAGKRRELPNDFVWVLAGTLPPNDFLQR
                     IGVHMGMQDLTQSARVEARRTA"
BASE COUNT         3543 a         7102 c         7652 g         3379 t
ORIGIN      
        1 acacgacggt gaaggtgacc gagcaggagg tgcaggacgc ggtggagcag acggtgcgca
       61 acgtgcgcgg ccgcttcacg tccgagacgg aattccagca acagctgcgg ctggccgcgt
      121 tcggcggggt ggaggagtgg cgccgttggc tcacggagaa ccagcgtcgc gagatcctgc
      181 gcacgcggct gctcgagaac ctgcggcagc gcgaaaagat caagccgatc ccgccgagcg
      241 acgcgcagct gcgcgcgtac tgggaggaga acaagggcca ggcgcagcag cgtccggcga
      301 acgtgtcgtt ccgccagatc gtcattctgc cgcggcccga ctccgcggcg cgggcccgcg
      361 cgcgggcgac cgccgaatcc ctggtcgtcg tgctgcgggg cggcgccgat ttcgccgaac
      421 tggcgcggcg gctgtcggcg gacagcacca cgcgggactc cgggggcacg ctcggatggt
      481 tccggcgcgg aacgatggtc aaggccttcg aggagacggc cttccggctc aggccgggcg
      541 agatctcgga cgtcgtggaa accgatttcg ggttccacat cattcaggtc cagcgcatac
      601 agcccgccga ggtgctggcg cgccacatcc tgatcgcccc ggagatttcc gctgtacaag
      661 tcgagtcggc gcgccgcctg gccgattcgg tgcgcgccgc gctcgcccgc ggggcatcgt
      721 tcgattcgct ggcgcagcgc tacggggacc agaacgttcc caagctggcg gaggcggtcg
      781 ccatcgccca gctgggaccg gaataccagc ggctgttcgc cgccgacagc acgaaggggc
      841 tcaagccagt gctcgagctg ggcgccggcg gcgcccgccc ccagttcgtg gtgctcgagg
      901 tcacagggcg gcaggccgaa ggggaagtga cgtacgacga cgtgcgcgac cagatccgca
      961 cccagctggc ccaggagctg ggggtccgtc actacgtcga tcagctgcgc cgccagactt
     1021 tcatcgacat ccggctctga ccgtggccgc cgcgcgccgc ccgcgactcg ccgtgacgct
     1081 cggcgatccg cgagggatcg ggcccgaggt cgtgacgcgc gcgctcgccg gcggaccgct
     1141 cggcgcggac gtcgtcctcg tgggtcccga cgacctcctc aacggcatcg cggcgcgcag
     1201 ccggatcggg gtcggaaact ggggacgggg aacgggggac gaggaacgag cgggtgagga
     1261 gcgcgcgcgg gcggcagggc aactcgctgt gcgggcgatc gagcgcgccg tctggctggc
     1321 gcgcgccgga gaagtggacg cggtggtcac ggggcccgtg gagaagcggg ccctccatct
     1381 cgccggctac cagttccccg gggtcacgga gctgttgtca cgtctcgccg gcggcattcc
     1441 gaccgcgatg atgctggctg caggtcccct gcgcgtcgtc ctcgtgacca cccacatccc
     1501 gctacgcgag gtgccgcagc gtctgaccac cgaggggatc gtcgcggtcg gacgactgac
     1561 ggcggcggcc ctggaacggt ggtgggggct ggccacgccg cggctcgtgc tgtgcgcgct
     1621 gaacccccat gccggtgagg gcggcctgtt cggcgacgag gacgatcgca tcctccggcc
     1681 cgcggcgcag gcgctcggtg ccgccgggcc gctgccggcc gacacggtgt tcgtgaaggc
     1741 gctgcgcggg atgttcgacg cggtgctcgc cccctaccac gatgtcggca tgacggcggt
     1801 caaggtggcc ggcttcggca agggcgtgaa cgtcaccctg ggattgccat ttccgcggac
     1861 ctcgcccgat cacggcacgg cattcgacat cgcggggaag ggcgtcgcgg atccggggag
     1921 catgcgggcc gccctcgagc tcgcgaccga gctggcgcag cacggccgcg tgcgggactg
     1981 aggccgggcg tcaggcggcg ggtcgcggct gcgtcacccg caccttgccc acccgccggc
     2041 cgtccatctc cagcacctcg aaggaggcgc cggcgatctc gagccgatcg ccgaccttgg
     2101 ggagccggcc cagctgcccg aacaggaccc cgccgatcgt cgtgtagtcg gtgtcgtcga
     2161 tctggagtcc ataggtccga ttgacgtccg cgacctcggt gccgccggcg atcaccggcg
     2221 cgggcgccgt cgcactgccg gcggcgacac tcgtctcggg ccggtcgtac tcgtcgtaga
     2281 tctgtcccac gatctcttcc agcaggtctt ccatcgtaac gagtccggcg gtgccgccgt
     2341 actcgtcgag cacgatggcc atgtggactt tttggcgctt catgtcggcc aggacgtcct
     2401 cgacctcgcg cgtgccgggc acgaagagcg tggggcgcat gatcgcctcg agacgttgcg
     2461 cggggtcagc ccgcagcccg gcgaggatcg ccttggcgtg caccacgccg atgatgtcgt
     2521 cgagcgattc ccggtacacc gggtagcgcg agcgacccgc ggccgccacc cggtcggcgg
     2581 cctggacgag ggtgaggtcg ctctgcagcg cgacgacctg cgtgcggggc gtcatgacgt
     2641 ctcgcgcatt cttctcgctg aactcgaaca ccccctcgat catgcgggcg tcctgcgggg
     2701 cgaggcgtcc cgttttgcgg ctctgctcga cgagcatcat gatctcctcg ggggagtgca
     2761 cgcgctcgtg ctcggccgtg ccgcccttga tgccgaacag tcgcacgatc cggttggcgc
     2821 tccagttcag caggccggtg aaggggcggg taatcaggga aaagaagatg agcggcgccg
     2881 cgatccagcg gctggtccct tcggggtagg tgatcgccca tgccttgggc gcctgctccc
     2941 cgaatacgac gtgcaggaag gaaacgatgg acacggcgac gaccgacgcg accccgccgc
     3001 gcgtcaggaa gctgagcgcc gcggggagcg ccgaaaacca tccgcgaaac aggtgggcga
     3061 ccgtgtcttc cgcgacgtag cccaggagaa tcgaggcgac cgtgatgccg agctgggcgg
     3121 cggagagctg ccggttcatg tcgtgcagcg ccaccaggac cgtcttcgcc ttccggtcgc
     3181 cgcggcggac catcgcttcg atgcgggtgc ggcgcgcggc gacgagggag aactcggcgg
     3241 ccacgaagaa ggcgttcacc aggatgagaa tggcgactgc cgcgaggcga aggcccaggg
     3301 attcagcggc gggcgctggc atagggaggg ggaaagtagc ggggccgagg gtgacatcaa
     3361 ggcgcgagtt gcctatcctt cggctggagc acacatgacc cagaccctgc cgctcgacgt
     3421 tcaatgcgcg catcgagtgc cggagcggag cgaaagcgcc cgccgcctcg catgggtgct
     3481 cggcctcacc gccacggtga tggtcgcgga agcggtcggc gggtggctgg ctcattcgct
     3541 ggcgctgctc gcggatgcgg gtcatatgct ggccgacgtg gtggcgctgg ggctcgcact
     3601 gatggtgcta cggatggcgc agcgcccggc gacgcctgag cgcacctacg ggctggttcg
     3661 gctcgagatc ctcgcagctc tggtgaacgg cgccgcgctg atcgcgatcg cgctgggaat
     3721 cggcatcgag gcggtgcgcc gcctgcaggt cccaccggcc gtgcatggcg gactgctgct
     3781 cggcgtcgcc agcgtgggac tcctggccaa cctggccggc gtcacgattc tccatcgtgg
     3841 tcatcaccat tccctgaacc agcgcggcgc ctatctgcac gtcatgagcg acctgctggg
     3901 atcagtgggg gcggtcctgg ccggcgtcct cgtggtcgcg accgggtggc ggctggcgga
     3961 ccctatcgtt tcgctcggga tcggcgtcct ggtgctgagc ggcgcctggc gcctggtccg
     4021 ggagagcgcc gacgtgctgc tcgaggcggc accggcgcac atcgccctgg gcaccgtcca
     4081 cgagcggctg gccagtctgc ccggggtgag ctcggtgcac gatctgcacg tctggacgct
     4141 gaccagcggg gtgatcgcga tgagcgggca cctggtagtc cgcaatcccg agcagaacca
     4201 gcagatccta gaggctgccc aggcccgcct ggagcagctg gggattcacc atgtgacggt
     4261 ccagatggag cgggacgtca cgtgcgattg accttgtatt ttcaggaccc tctccctata
     4321 ctgccgccct cgcaacccac actcgtcagg acgtagctcc ttgcagatcc gcaacatcgc
     4381 catcatcgcc cacgtcgatc acgggaagac caccctcgtg tcccagatgc tccggcaagc
     4441 ccgagcgctg cgacacgggg ctacgggttt cctcgatacc gggatcgagg ccgagcgggg
     4501 catcacgatc ctttcgaaga acacggcggt ccgctggggc gagacgaaga tcaacatcgt
     4561 cgacacgccc gggcacgccg atttcggggg cgaggtggag cgcatcctgc ggatggtgga
     4621 cgggttcctg ctgctggtgg atgccgcgga gggtccgatg ccgcaaaccc ggttcgtggc
     4681 ggggaaggcg ctggcgctgg ggctcaagcc gatcgtcgtg atcaacaaga tcgatcggca
     4741 ggacgccgag ccgctgcggg tgcatgacga ggtgctgcaa ctgtttctcg acctcgaggc
     4801 gacgccggag cagttcaact gtcctttcct gtacgcctcg agccggcagg ggatcgcgac
     4861 gccggatctc ggcacgccgg ggaccagcct gaagccgctg ttcgagacga tcctggccac
     4921 cgtgccgccg ccctcgggcg atccggacgg cccactgcag atgctgattt cgacgctcga
     4981 ctactcgtcg tacctggggc gcatcgggat cgggcggatc gagcggggac gggtccgcgt
     5041 gggggagacg gtggcactgc tgccgttggg catgcccggt ccggtggggg acgggaccta
     5101 cgaacaggcc cgggtggtga aactgttcgg gttcgagggg ttggaacgcg tggagctgga
     5161 ggaggcggcg gcgggcgaga tcgtggcgct ggctgggctc tccggggtgg agatcggcaa
     5221 gaccgtgacc gcgccggacc acctggagcg actggcgggg atcgcagtgg aggagcccac
     5281 tatttcggtg gatttccggg tgaacgacgc gcccttcgcc gggcgggagg ggaagtatgt
     5341 gacggggcgg cagctgcggg accgtctcta taaggaactg gagcgcaacg tggcgctgcg
     5401 ggtggaagac accgacgacc cgcagacgtt caccgtgtcg gggcgcggcg agctgcacct
     5461 cggtatcctg atggagacga tgcgccggga gggcttcgag ttccaggtga gccggccgcg
     5521 catcatcccg cgcgaagggc cgaacgggga gcagctcgag ccgtacgagg agttgctgat
     5581 cgactgtccg gaggggtatc tcggtgtggt gatggagaag ctggggccgc ggcgagcgac
     5641 gatgaaggat atgcggaacc ccggggtggg gtcggtgcgg ctgcgattcg ccattccggc
     5701 gcgcggcctc ttgggctacc gttccgagtt cttgacggac actcgcggaa cggggactat
     5761 gcatcatcgg ttcctggagt acgggccgtg ggcggggccg ctgtcggggc gcagtcgcgg
     5821 cgtgatggtg gcggaccggg aaggggtggc ggtggcgtac gcgctgttca atttgcagga
     5881 acgtggtacc atgttcgtga agccggggga cccggtgtac gaggggatga tcgtggggga
     5941 gcatgtgcgg ccgggggacc tcgacgtgaa cgcgacgaag gagaagaagc tgacgaacat
     6001 gcggaccacg gcgtcggacg agatggtgat tctggagccg ccacgccaga tcacgctcga
     6061 attggcgctg gagtacattg aggacgacga gctcatcgaa gtgacgccgt cgtccgtgcg
     6121 gctccgcaag cgcgagctcc aggcgaccgc acggcgcaag ctcgcccgct cgttgcgggc
     6181 gatggacgag taagccaacc gcaacctcag ccgggggtga cgatgctggc cgtggcaacg
     6241 tcgatggggt ggtcgctcaa gcgtcgcagc gacgacatcg acgaggagtt cgaggacttc
     6301 gaggaggaca aggagctcga agaggacttc gacgacgagc tggacgagga cgagctggac
     6361 gacgaggaaa tggaagatct cgacgactac gacgacctcg acgacgagtt cgacgacgaa
     6421 gacgaggacg gcttccggcc caagaagggg ccgaagcggg aatgggactg acggcagggc
     6481 ctgtagctca ggtgggtaga gggcacgcct gataactcct gacggccgac gcacaccagc
     6541 gtcggccgtc gtgttttcaa gcacttctgc catcgcgacg cgcgtcagcg ccaatcacca
     6601 acggcgtcgt tggcaccacg gttggcacca cgacggactt tcaagaattc ttaacctctc
     6661 tggcactact ttgcgcttga gcatgcagga acctgaatcg cgtcgccgaa tggctaagcg
     6721 agacacggta tgcaaagggg aggcagccat gacacgcaag ctagcgggcc ctctggttgg
     6781 ggccctcggg gtgctcgctg ttgcagtact cggcatacag gtcgctttcg cacactgtga
     6841 cgggctcgac gggccagtcg tgaaagccgc tcagagcgcg ctgcagacgg ggaatgtgaa
     6901 cctggtcctc gtatgggtcc aggagaagga tgagcccgag atccgggcgg cgttccagca
     6961 agctttggtc gtgcgcaaac aaagccccga agcgcaggct ctcgccgacc gctctttctt
     7021 tgagacccta gtccgcattc atcgcgccgg agagggggcg ccgtacacgg gcctcaagcc
     7081 cgccggacgg gatctcggcc ctgcgatccc agccgcagac aaggccctgg aaacgggcgc
     7141 tgtgggtccg cttcaggatc ttctggcgaa cgcggttcag catgggctca aggagcgctt
     7201 cgaggcgctc agaaccctga gaggctatga ccccaatgat gtcgcggcgg ggcgccggta
     7261 tgtgcaggcc tatgtcgagt acgttcacta cgttgagcgt ctccacgaag ccgccacggc
     7321 cgcggcggaa ggtcactacg ccgaagcgcg tacgggtgag ccccaccgct agccgacaat
     7381 ggcgattcgg ccttcccgca tagcggccct gctgattcat ctgtctttgg atccttcttc
     7441 atagcccttg gcgtagttag gaggattgtg gcgtcgtgcg gcgggtgggc gacgccgcgg
     7501 ctggtaggca cccgggattg cgaggacagt atggttgcgc agatgcggcg tcttggacgc
     7561 cgaacgcgcc acggcgcgat ctatgaacgt ggaatgttgg ccctaatgct gttgacgacg
     7621 ggcgccgtgt tcagcggatg cggaaagacc cgagcgtctc ccggagacag tgcagtcaac
     7681 ttgggaagcg cggccgattc ggtcgccgag ggccagggca tcttccgctt cgatacgttt
     7741 ggtgacgaac aattctggac cgacacgctc cacatgaacc aggtcgtcga gcagagcgtg
     7801 gacccgacca cggcgctcgc ggtcgggctc aaggtcgacg ccgatgtcct gcctccgggg
     7861 attctccagc aggtcgacct caagagcccg gcgacgacgg tggcgctgct caaaatgaat
     7921 gccgtcgtag gtatccaggc cacggtggac tcgaacaacc acatcacgcg cctgggcatc
     7981 acctgcgcac tatgtcactc aaacgtggac aactcggtca tggctggcat cggccatcgc
     8041 ctggacggct ggccgaacca cgacctgaat gtgggggcca ttgttgccct gtcacccgtc
     8101 ctcagcgcgg ctcagaaggc agtctaccag tcgtgggggc cgggaaagta cgacccgcgc
     8161 tacaacctgg acgggaagaa cggggcgctt gttatcccgc ccgcctacgg gttggcgcag
     8221 gtcaagaacg aaacgtatac ggccgaggga ccgatttcgt attggaatgc ctacgtcgcc
     8281 gtcactcaga tgcatgggca cggcaacttc tcggatgcac ggctcgggat cagggtcacg
     8341 cagtcgccgg acatggtcgg cccgaagctt gcggcgctgc gtgcctacca gcatagcctt
     8401 gccgccccgc ccccgcccgc cggaagcttc gatacggcag gcgcgcaacg cggacgtgcg
     8461 ctgtttgcgc ggatgtgcac gagctgccat gtgggtgtga ccggcactga taacaacgat
     8521 gggaagctgc atgcacctgc cgaaaccggc gttgacggag cgtacgccgc tcgtacggca
     8581 accaaggcat accgcacgac cccgctgcgt gcgttgtggc agcacgcgcc gtatttccat
     8641 gacggcagcg cggcgacgct tcccgacgtc gtgacgcatt acaactctgt tcgaaagctc
     8701 gggctgacgg cagcgcaaca acacgacctg attgagtatc tgaagtcgtt ttgagggtcc
     8761 ggggcatgga ctctcgattc gtaccctctg acggccgacg cacaccagcg tcggccgtcg
     8821 tgttctcaat gcgctacgtg tggctaccgg cggtcaaccc tcgtagactc ctcttccgac
     8881 cttaccgcac gggcgagcag gtgcggcacc gggccgaggc ggtggagtac cgcgtcgagc
     8941 aacaccgggc taagttggag gaccgcttac gggcgttgtg cggggcagat tttggcacca
     9001 ccattggcac cacgaggtta gagctcccgc ctaatgctgt aagtcgttgc agtacatgag
     9061 gggcctgtag ctcaggtggt tagagcgcac gcctgataag cgtgaggtcg gtagttcaac
     9121 tctacccagg cccacttaag gagtcagccc ctctcgggtc atgcccgagg ggggcttctt
     9181 ccatccgaag ggctatgtgt gcaacgatga cacgtgtgac atcgcccgtc actgtccgcc
     9241 gatcgacgcg ccgatctgcc gggcgatacc aaccaggccg ccatcgggcg gcgtgacgtt
     9301 cgcccagctc gcgagcttgc catcgcgaac catcaccgtc gcgctctccg gcaccagttt
     9361 caccgacttg ccagtgggtg cgaaggctcg tacgcccagc ggggcaagat ccagcgtgcc
     9421 ggtgtgcgtc atcgtcagag ccacgccaaa atgcacgcca ctgcccgtcg tctgaaagcc
     9481 tgacgcattg aagcgcaggt cgggcatcgc cgcgtacagc gcccggtgca cgccgatcca
     9541 cgcatcgggc ccgatcggct gcggcaccgg accactgaag gtaaagtcgt cgtgcagcag
     9601 cgaccgcgcc tgtgcgaact gtccggcttc gatggcggcc atgacggaac gcacgaccga
     9661 ttcagcgggc tggctcgcgg ctggaacccg ggcgtatatc tcgaaccgct ccaccttggt
     9721 gggcgtgggc cgctggtgct tgctgcgcgc cacccggtcc tgcacccgcg ccatcgcggc
     9781 cgaggcgtgc gcctcggatt cccagaccat gatggagatg cgcttgccat gctcgcggtc
     9841 cacgagccac aggccggaga gcccgggtga cgtctcgagc gcgggaatca cgtcgtcgag
     9901 cacatggtcg atgccagcct gcacctggtc cggactctcc tcgaacgtca cgacctgcgc
     9961 gtacatgggg cctccagacg gagaagggga gaaccgctct tgtattttcc tttcgtaaga
    10021 aagtatattt ggccatggcg aagcgcgcaa gagcgaggga cgctccgcag tttctcgacg
    10081 acgacgaagt ggcggcctgg cacggcctgc tccagacgag cggccgggtc ctccgcgaac
    10141 tcgaccagag gctgcagcgg gagcacacca tctcggtggg ggagtttgac gtgctgatca
    10201 cactgttcaa cgcaccggat cagcgcctgc ggatggcgga actcgcgcga agcgtgtcgc
    10261 tgagtccagc cggtctcaca cacctcgtcg cgcgactcga gcgggagcgg ctggtgcggc
    10321 gcgcgacgga ccccgaggat cgccggggat cctacacggt gctcacggca aaggggctcg
    10381 cccggctcaa cgccgcgcga ccggtgcaca acgaagtgat tcgccatctg ttcctgaatc
    10441 ggatcacaac ggacgaccgg cggcggcttg cggtgatatg gaggcgggcg cagggatctg
    10501 ctcctcgagc tccggcgtcg tctggttcgg catccgcggg cgccgggggg ctggggggcc
    10561 aggccgcccc gcggtagcgc tcgaacggtt tgccgatgag cgtgaggtcg gtagttcacc
    10621 tgcgacccag gcccgctcca cgcccaacgg aggcaaaccc acatgcgcgt gctgctgtcg
    10681 acaatcgggt cgcggggcga tgtccagccc ctggtggcgc tggcgttgca gctgaaggcg
    10741 ctcggtcagg aggtccgcct gtgcgtgccg cccgacttcc gagactggat cgagagcctg
    10801 gggatgcccg tgacgcccat cggacccgag ctgcgatcga ccgggaaagc cagcccatca
    10861 gccgctctgc ccacgcccga gcagcggcgt cagatgacgg aagccacggt cgccgcccag
    10921 tttgcgacga tcacgatggc agcccaggga tgcgacctca tcgtaggggc gacggcccta
    10981 cagatcgcgg ctccctcggt ggccgagcgg atgagcatcc cgtatgtctt tgccgcgtac
    11041 tgcccgaacg tgctgccgtc gccacaccac gcgccgcccg tctattccct tcagggagac
    11101 gcgcccacgg ccgcaccggc cgattactcc gagctctggg cccggaatgc cgaacgcttc
    11161 aacacatcgt tcggtgcggc cctcaacgcc catcgcgcgg cactcggtct ggcgccggta
    11221 ggtgacgtgc gcagccacat cttcaccagc cggccctggc tggccgccga cccgacgctg
    11281 gcgccctggc ccgatcccgc agacgatgcc gtgtttcaga cgggcgcctg gatcctgccg
    11341 gatgagcgtc cactctcccg agagctggag gcgttcctcg aggccggcga accacccgtc
    11401 tacttcggct tcggcagcgt acgggcgccg gaaggcctca gccgggtgat gatcgaatcg
    11461 gcccgtgccc tcgggcgccg tgcgatcgtg tctcgcgggt gggccgacct ggcgctcgtc
    11521 gacgacgagc ccgattgcct ggccatcggc gaggtcaatc agcaggcgct gttcaaacgc
    11581 gtcgccgccg tggtgcacca cggcggctcg ggtaccacta ccgtagccgc gcgagccgct
    11641 gcaccccagg tcgtcattcc gcagcactac gaccagcact actgggccca gcgcgtccac
    11701 cacctcggca tcggaaccgc gcacgcaccc ggcacaccga ctccggactc gttgacgagc
    11761 gctctcgagc acaccctcca gcccgatgtg gctacccgtg cccgatccat cgccaccgta
    11821 gtgcgcagcg atggcgcaca cgccgccgct ctgtacgtga tggtcgccga cccacggaac
    11881 tcactctgaa acccgtgtcg ctcggcctcg cctggccggt gcaggggcgt cttccggaca
    11941 cgctgtgcct gtgatcgccg accccagcgc gcaccccggg gctggggcct tcctcgtcgg
    12001 gctcttcggt caggcgtcac cgcggactca ccagcgatgc actccatcca gccgatggga
    12061 tgcggcggtc agcgcccggg acgagaacta gcgatattat gcgttcatcg ccctgcgcca
    12121 ggccttctcc tgccggcggg ttgtcctcgc gatccaaagg agtccccgaa tggtggctgc
    12181 cgtgccgatg cagcatcccc ccagagtgag tgagctcgtg cgtcgccggc tcaaggagct
    12241 ggagcgcacg ccgctggagc tcgccgaggc gctgcaggtt ccggagtcct acgtcgcgga
    12301 tctgctctca gggcgccgcc gtccgcccgc gcccggccgc accgacatct acgaccggat
    12361 gacgaagttc ctgcggatgc cccgcaatga gctgtccacg tgcgcgcgtg ccgagctggc
    12421 ggcggatccg agccggcagc gcccccccga ccagaaagtg aagaagctgc tgctcgagct
    12481 gtgcgagccg acccgggcgc gcacgctgag ccgccggctg gccgacaaga agggcaaccc
    12541 ggcgctcgag atcctcatcg cgcagcggct gctcgaagtc gctcagggat tcgtgcgccg
    12601 tcagctggac gacgaggtcg gcatccgggt ggcggccacc cgggaaggct gcacccacag
    12661 cgagatgcgg ttgcggctca tcgacttcct cgacgccacg gccgactcac tcaccgtgac
    12721 ggaccatgaa gtgtacgtgc ggccccgcat cgcgacctgg gacatcgatc tcgagacaca
    12781 cgcgatgaag atcgtgctgc gttcccacga accggagccc cgacagcgtc gttggactcg
    12841 gtgagacggt aatcgaccac accgcacccc tcctcctcac cccggaagca acccatgcgt
    12901 tgctggctgc tgtgcgcgtg cgccctcctc tttccgccgg cgagcggtgc cgcccaggaa
    12961 ccgaccccac ggacgttcac acacgccgac tcgctgcgcg gcgccgacac accgcagcgc
    13021 gcatggtggg acgtgacctt ttacgatctc cacgtcgcgg tgcatccggc cgacagcacg
    13081 atcgcgggct acaacgggat cacctatcgg gtgctgcgcc cggcgcggga gatgcaaatc
    13141 gatctgcagg agccgctcac cgtcgacagc atggtccagg acggccgccg cctctcgttt
    13201 cgccgcgacg gcaacgcatt ctttgccacg ctcggcacgc cgcagcgcgc cggcgagcgg
    13261 cgcacgatca ccgtctatta ccacggcaag ccccgcgcgg cggtacgccc cccctgggac
    13321 ggcgggctga tctgggccca ggacagtgcc gggaacgcct gggttgcgac cgcgaacgaa
    13381 gggctgggcg ccagcgtctg gtggcccaac aaggactacc tggccgacga gcccgacagc
    13441 cagcgggtgg cgatcacgat gcccgacccg atgttcgacg tctccaacgg ccggctgcgc
    13501 ggcgtgaccc cccatcccga cagcacgacg acctacgagt ggttcgtcac gcaacccatc
    13561 aacaactacg acatcgcgat caacgccggc gggtacgccc acttcgcgac gatctacagc
    13621 ggcgaagcgg ggccgctcac gatggatttc tggccgctgg cgtatcaccg cgcagcggcc
    13681 gagcggcagt tcacgcaggc ggagtccatg atgaggtgct tcgagtcgtg gttcggaccg
    13741 tacccctggt acgaagacgg ctacaaactg gtcgagacgc cgcacctcgg gatggagcac
    13801 cagaccgcgg tggcgtacgg caatcactac gggaacgggt atctggggcg cgatctctcg
    13861 cataccgggt tgggactgct gtgggacttc atcgtggtgc acgagagcgc ccacgagtgg
    13921 ttcggcaaca gcatcacgat gcgggaccag gcggacatgt gggtgcacga gagcttcgcc
    13981 aactactccg agaacctcta caccgagtgc ctggcgggaa agcaggccgg cgcgcagtac
    14041 gtgatcggca cgcgccagaa catccgcaac gaccgtccca tcgtcgggca gtacggagtc
    14101 aacgaccagg gctccggcga catgtactac aagggcggca acatgctgca cacgatccgc
    14161 cagatcgtcg gcgacgacgc gaaatggcgc agcatcctgc ggggcctgaa cagcacgttc
    14221 cgccaccaga cggtgaccgg gcagcaggtc caggattaca tcagccggga atcgggcgtg
    14281 gatctccgca aggtgttcga gcaatacctc acgacgacgc tcgtcccggt gttcgagtac
    14341 agcatcgtcg actcgacgct cgcctatcgg tggactcagg tgatcgctgg attcgacatg
    14401 ccggtgaagg cgacggtcac cgactccggg ttcaccctgc tgcgcccgac cgagacctgg
    14461 cagcggacgg ccatccacct gaaggacccg gtcaccttcc acctggacca gaatttctac
    14521 gtcctgaccc ggcacgacgt cgccccgccg ccgcaagccg gccggtaagc ttggatgacg
    14581 gggatcctgc ccggcctcct gctcgccgtc tgctgtgtcg ccggcctggt gctcgtcgct
    14641 ctggggctgc ccggcctgtg ggtgatggtg ctgggcgtca tcgcgtacgc ctggatgacg
    14701 ggattccaca ccatcggggc ggtgacgatc tccgtcgtgc tggggctcgc cgtggtggga
    14761 gagatcgtgg agtggtgggt gggattctgg ttcgccgagc gctacggcgg ctcgcggcgc
    14821 gccgggtggg gggcgctgct gggggggatc gtcggcgcgg tggttggcgt gccggtaccc
    14881 gtcatcggga gcgtgatcgg cgcgttcgtg ggcgcgttcg ccggcgccgc cctgttcgaa
    14941 tacacgcggc agctctcgcc cggggtcgcc ttgcgtgcgg ggtggggcgc cgtgctgggg
    15001 cgggccgccg cctcagcgct gaagatcgcg ttgggactgg cgattgccgt cgtggcggcg
    15061 ttcgaggcga ttcgcggtta gtggcaggga agctgcccgg cgtcgctgaa cgtccttccc
    15121 gggacgggta cgaacagcca ctcgtagctc gtgggatgga gcaccaaccg cagcacgccg
    15181 tacgagtcgt cgttctgggc gacgctgttg agcgcgaggg gcaggttgat ccggccgtgg
    15241 ctcttgccgc cggtccccac gaccagctgc accacgcccg ccgcggaatc gagggtctgg
    15301 tccggggcct gcggcgcgaa gcgctcgtag tcatggtcgt tgccggatat cagcagctcc
    15361 accccatggt cgtacagcgt ctgaaacacc gcccccatgc cgggtgagct gccggtattg
    15421 ccggagctga accgcggccg gtgtgcgatc gccagcgcac accgcgtggg gtgcgccgtc
    15481 aggtcagccc gcagccagct cagctgggcc gtgtcgacca tcggcgtggc gttgagcacc
    15541 acgatgtgcc aggcgccaag gtcatagctg taggccccgc tcgggggatg tgcggcggcg
    15601 ccgaagtagg cgtagtagtc gtccagcgtc aggttcccgc cacgccggtc gtcgtcaccc
    15661 ggcgcagggt ggaggcgcgc gcggtgacgg ccccacgtcg agtcgaaaca cacgccatac
    15721 gccggcgcca cgagaccgtc ggagtaatcg ttgtcgcccg cggtgaatac ggcgcccggc
    15781 agggtatcga gcagccgggc agtggcttca tcgttcgtgg agacgcaact ggcgatgtcc
    15841 ccggccccca cgaggagcgc gtcggactcg atcctcgggt ccgtaccgtt gccacaggcg
    15901 accaggagga gcagggcagc caccgataat ctcatgggac aaatatcggc ctggttgccg
    15961 gagggataca aggcagcggg tcacagtctt acctttgaac accgtgcgct tccttcatgt
    16021 cgtcgaacga gacgggcgct atgccgcgga actgcgcgaa gcgcccgacc cggtgccggc
    16081 ggctggcgag gtgctggtgc gagttgccgc gtccggggtg aatcgcgccg acctctcgca
    16141 aatcgctggt cgctatgcgc cgcctcccgg agaatcgagt attctcgggc tcgagatatc
    16201 cggaacagtc gtcgagaccg gcgcaccggt gtgcgcgctg ctgggcggcg gcggacacgc
    16261 ggagctggtg gcggttccca ccgggcagat ccttccggtt ccccccgccc tggatctcgc
    16321 gatggcggcc gctcttcccg aagcctatct caccgcattc gtgaatctgg tcgtggaggc
    16381 cgggctccgg gccggggatc gcgtgttgat ccgcgccggc gcctccgggg tcggcctcgc
    16441 cgcgatccag gtcgcccggt acgtcggcgc gcaggtcgcg gcgacgacgc gctcgcccga
    16501 caagcgagcg gcgctcgaag gggtcggcgc ggatctcgcc atcgtgacaa cggcagacgg
    16561 agggacggcc gagatcgagc ggcactgggg ccgcgatgcg gtggacgtcg tgctggaccc
    16621 catcgggggg tccagcgtgg ccggcgatct ccagctgctc gcgcccggcg gccgtatcgt
    16681 ggtgattgcg gcgatggacg ggaccagtgc cgagatcgat ctccgcctgc tgatgaagaa
    16741 gcgagcccgg ctcgtgggct cgacgctgcg cgcgcgcagc cgggccgaga aggcccgcct
    16801 gaccgcccgc ttccgggacg aatgcttccc cggtttcgag gcggggcgcc tcggcgtgaa
    16861 cgtggaggca gtatatggac cggaggatgc ggcgcaggcc tatcagcgaa tgcgggaaaa
    16921 ccgcaatacc gggaagctgc tcatccggtg gccggcctct tgcgagtcat cggagcggcc
    16981 cccatcgtga gcggaccagc actcgaccga ttcgcatgac cgtcgcgcca tctcgccgtg
    17041 ccgcacccgg ccgctcgagc ggaccgggac cgtcgctccg cgcggtgctc gacgcggcgc
    17101 tcgacgccgt catcctcatg gacgacgacg gcgtcatccg cttctggaac cgccaggcgg
    17161 aagcgctgct cggctggacg gccccggagg ccgtcggacg gtcgttctcc tcgctcatcg
    17221 accccgagcg accggcgggg cgccggaagc acggcgtggc gggccggacg gccgacgagc
    17281 ggcggcaggc cgcccgccgt cgcgaggtga cggcgcgcca tcgcgacggg cacgtggttc
    17341 cggtcgagct gggaatcaca ccgatccacg acaggacgag tcggttccag ggtgcctggc
    17401 tgcgggacat ttcggagcgg cggcggcggg aagcggtgga gcggttgcag tcgtgcattc
    17461 tcgagaacgt ccacgacagc gtcatcctcg tcgacctcca gggccgcgtg caatactgga
    17521 atcagggtgc ggaggctctg tacggctaca aggcgtcgga gatgctcggc cgcagcatcg
    17581 gggccatcct cccggagggc gaggctgtgg aacccgacct ggagcatgtc ctggcgaccg
    17641 gtgatacctc ggaggaggca cggcggcgcc acaaggacgg cacgccgctc tgggtggaat
    17701 cgcgccggtc ggtgatgcgc gatgcggcgg gggacgtgct cggcgttctg ggcgtcgtca
    17761 aggacgtcac gcagcgccat cgggtggccg aggagctcga gcgctcgcga gcgcagctgc
    17821 ggaatctcgc cgtgcggctc cgccgggccc gggaggagga gcgcacgtcg atcgcccgac
    17881 agatccacga cgagatgggg caggtgctca ccgcgctgcg catggacgtc gcgtggctgg
    17941 aagcgcgcat cccgcgggac cagccgtcgc tgctcgacaa gtgcgccacg atggcgcgac
    18001 tcattgaggg aatgatcggg caggtccgga ccctggcggc gcagctgcga ccgggggtgc
    18061 tggatgagct ggggctcgcg gcggcgatcg agtgggagac tcaggagttc gcgcggcgca
    18121 cgggcatcgc ctgcaccctg cacctgcgcg ccgagctggc cggcgtcgat cccgaccggg
    18181 cgatcgacct gtttcggatc ctccaggaag cgctcatcaa cgtcgcgcgg catgcacggg
    18241 cgcagcaggt cggcgtgcgc gtcagcgtca cccggaaagg aagggagcgg gaggtggtgc
    18301 tgcacgtcga ggacgacggc cgtggcatcc gaccggagga gaccgcggat gcgcgctccc
    18361 tcgggctgct cggcatgcgc gagcgagcgt tgttgtggaa cggcggcgtg gaggtgcgac
    18421 ccagggccga aggcgggacc cgcgtgaccg tccggctccc gctgcccgcg acggggctat
    18481 ccgcatgaac cggccgatcc gggtcgcgat cgcggacgat cacccgctgg tgcgggcggg
    18541 gatcaagcag gtgattgcgg acgacgagac gctcaccgtg gtgggcgagg cggccgacgg
    18601 cgacgcgacc gtcacgatgg tccgctcgac cctccccgac gtgctggtgc tggacctgtc
    18661 catgccgggc gcgccgttcc ccggcctgct gcgcgtgctc cgggccgcgt ttccgacgct
    18721 ccgcatcctg gtcgtaacca tgcacaccga ggagcagttt gcggcccgtg ccctgcggga
    18781 aggagtggcg gggtacgtca ccaaggaccg gccgccggac gacatcgtgg cggcggtcag
    18841 gcaggtcgcc gcgggcggac gctacatcac ggcagctctg gcggacagcg tcacggacgc
    18901 cgcgaacgcc gagctccccc atgagcgggc gtcgcagcgt gagtacgagg tcctctgcat
    18961 gatcggctgc gggaagaccg tcaagcagat cgcgatcgag ctgggcttgt cgcccaagac
    19021 aatcagcacg caccgcgcca ggctgctgcg gaagatgcag ctgaagtcga gcgcggagct
    19081 gatccgctat gccgtccagc acgggctgag cagctaagtc agcgacgcgc ctcggtcacg
    19141 cctgccacgg cagacccgag gatgtcggcc aggtccttgc ggtagtccgg atcgttcggg
    19201 tcgatcgccg gccagcgggc gggatcgtcc ggcgcgaaca accccaccat cagccccatc
    19261 atcccggtga gcgtatcggt cgacggattc tcgtactcca cctcgagccg atagcgatgg
    19321 tcggcgcgca gatgcgggcc ggccccgcgc agggcgagca acgcccgtga catgccgacc
    19381 acgtgtccgg tcgagtcccg ccgggagcgt accgacacca ccgtccgacc ggtcgtccgg
    19441 tcgagcaggc tcacccggac gccatgatcg tgcagatggc cgctggcggc gacgaggtga
    19501 ccgtcgaggg gcagcgtgaa gtcgaatcgc tgggtcgtcc cgccaggggg gacgacgaac
    19561 gtattgtgtc cagccgggac caggtgcgca tcgatggtga acggcatgac ctcggtcggc
    19621 cggggcacga ggttcccggg agcccaccgg aaggtgagct gtacgtacac atcgtcgagg
    19681 tcgcggccgg tcttgttgtc ccacatcacg aacacaccga tgcgctggcc tgcggtcatg
    19741 gggaggccga tcgagcttgg caccgacacg tcctcggtct cctggccgaa ccccatcatc
    19801 cgctcgacca acgggtagac cagcgcgcgg cgatcgaagt tcaccaggtt cagatggtgc
    19861 agcagacccc ggggcagggg ccggcccgcg ccgtcgctca ggtcgagccg gaggccctgc
    19921 agcagggtgg tcatcggcca gtcgaagcgc ttcacgaggg aatcgtcgcc tgccatatgc
    19981 tccatctcca tgtcgggcat catcggcatg gcggaggcga catggaacgg gccggcgagg
    20041 atcacgaccg tgtggcgggt cgagtccaca cgcacacccg gtggaagacc ggcgctccgc
    20101 tccccgattg ccgcagtggc acgagccggc gccgtgagga aggacacgaa gacggcgatc
    20161 ccggccacgc ggacgacggg gaagtgcatg acggcctctg ttgtggagga gatgctcgac
    20221 cgaccgaaga taccgtgcgg gcgtcgtggg cgcgagtcag gccgtgcgac gagcctccac
    20281 gcgcgcgctt tgcgtgagat cctgcatgcc catgtgcacg ccgatgcgct ggaggaagtc
    20341 gttgggcggg agcgttccgg ccagcaccca cacgaagtcg ttggggagct cccgccgctt
    20401 gccggcgacc tcgagcacta cgacgcgctc gtggatctcc acgggttgcg aatcgaatac
    20461 gacggtgagc cttcccgact tcacgaattc cttcaggcgt tgagcgttgc gctccttgat
    20521 gcggctgaat tcgtgctggc gataggaaag ggtgaccgag ttgccgcgct ggtgggcgag
    20581 acccatcgcg gtctctaccg cgctgtcgcc gccacccacg accaggatgc ggaccccggt
    20641 gtacgccccc gcgtcgagca ggctgtacat cacctttggg agctcctcac ctgggacgcc
    20701 cagcttgcgc gggctgccac gccgcccgat cgccaggatc acggcgcgcg accggtagcg
    20761 tccgcgcggt gtggcgatcg tgaagaatcc gtcctcctcc cgggtgatcg tgtcgacctt
    20821 ttccccggtg cgtaccgcga gccccgcctc cttgtgaatc ttgttccaga atttgagcag
    20881 ctcctccttc gacagctgga gcttcttgaa cttgccgtac acggggaact cgacggggct
    20941 ggtcatcacg accttctggc gcgggtactt ggcgacggtg cccccgaact cgtcctgctc
    21001 gagcgtcacg tagctgagct tgcgctcgac cgcccgcagc gacgcgctga tgccgcccgg
    21061 gccggcgccg acgatgcaca cgtcccgtac accggcgggg gcgtggccgg aacgggtcag
    21121 ccgctgcgcg atggtgtcga tgcagtcacg gccctggttg acggcgttct tgatgagagc
    21181 cagcccgccg agctcgccga ccacgaacag ccccggcacg ttggtctcga gctgaggcga
    21241 caaccggggc aggtcggcgg cgatgctggg gctggccatg acgatctcga tcgctcccac
    21301 cggacaggcc tccgcgcaca gcccatgacc gatgcacttg tgagcgctga tgatcgccgc
    21361 cttccccgcg atgaccgcca ggacgtcttc ggggcaggcg tcgacgcatg tcccgcagcc
    21421 gatgcagtgg gagacgtcga tgtgcggatg ctgggcgcgg ggacctgtgg gggacaaccc
    21481 cgcccgccgg gtggcggcca gcgccgcagc ctcccggcgg cgggcacgca ccacactgga
    21541 gagcaccagc aacccgacga gaactatgaa gccgatgagc gtgaaggagt tgtccatgtc
    21601 gtgaaacgag ccagttgaag cgatttgccc cacgagtcgt ggtgaccgca ccgtcgggtc
    21661 acatgccaac agcttg
//
LOCUS       DBNGZW010000545         2791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1742505, whole genome shotgun sequence.
ACCESSION   DBNGZW010000545 DBNGZW010000000
VERSION     DBNGZW010000545.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1742505"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..953
                     /locus_tag="AC16JC_13020"
     CDS             168..953
                     /locus_tag="AC16JC_13020"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5165664.1"
                     /translation="MTISSALTHAEAERLAGRIAELEHERKHLLAVIEILKEISGSLH
                     FIDILQSIARKLGESFGLDRCSIFLAERGGTHVRLVASYEDPTIRNHLVDLERYPELK
                     RSIHHGETVFIPDAMTDPTLKHVKNELSSRRVKSITVVPINWRGVAIGAIFLRTFRDG
                     PTFSDSDVRFVQVVASLTAQALRNAHRYERLAQRQAETGETLRRMELERVALLSFLRR
                     LMETFGRSDGAWGEGLLPKASAEELDRLVGVALAVIQEEAKGQ"
     gene            950..>2791
                     /gene="ligA"
                     /locus_tag="AC16JC_13025"
     CDS             950..>2791
                     /gene="ligA"
                     /locus_tag="AC16JC_13025"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR5165665.1"
                     /translation="MTAPAARAAELRRQLERANHAYYVLDRPEISDAEYDRLFQELQG
                     LEARHPELRTPDSPTHRVGAEPSPAFKKHRHLVPMLSLANAFDEAQLTAWEERNARLV
                     ADVAETGYTLEVKIDGAAVSLTYADGVLVTGATRGNGVEGEDVTPNLKTVLDLPLRLQ
                     GKGWPTRMEVRGEVYLSKSQFERVNKERAKEGEPLFANPRNAAAGALRQLDPRITRAR
                     GLRVFCFHVEAPRQTLPCDTQHGLLQQLSTWGFPVEPHHQLVPNLAAAHRAIAKLEKT
                     LPQLDYGADGVVVKVNRLALHAELGTVGDREPRWAIARKFAPEVAVTKLLEIRINVGR
                     TGALNPYAVLEPVEVGGVTVSTATLHNADLIAAKDIRIGDWVEVTRAGEVIPQVLGPV
                     RAKRRGKEKKFRMPNHCPVCRSRVERPQDEVMSYCPNVSCPGRIYEGIVHFASRGAMD
                     IRGLGYERVKALLDAKLIDDVSDLYDRKQVNLLKLLTLEGFAEKSAQQLLDAIDASKQ
                     RPLSTLLFALGLRHVGVQGARVLARHFGTMSRLAEATPEEIGDVRGVGPTIAEAVAGF
                     FAEPRNRKLVERLATLGLTMTEPRAAARTGGGPLAGQTYVITGTLPEL"
BASE COUNT          455 a          993 c          952 g          391 t
ORIGIN      
        1 atggacccct actacaagcg cgtcgccgcc gaggcgctcg cggacctgcg caagaacaag
       61 atcgacgccg cgcaggaacg tctgcaccag ggttagcgcc aagctaccgt ggcccaacac
      121 cctcagcaac ttgacgccgc gcgcgcgcgg tcgccattgt atcgggcatg acgatctcgt
      181 cggcactcac ccatgccgag gcggagcggc tcgccgggcg catcgccgag ctggagcacg
      241 agcgcaagca cctgctcgcg gtcatcgaga tcctcaagga gatctcggga tcgctgcact
      301 tcatcgacat tctgcaatcg atcgcgcgga agctcggcga atcgttcggg ctggaccgct
      361 gctccatctt cctggcggag cgcggcggga cccacgtgcg cctggtcgcc tcgtacgagg
      421 atccgacgat ccgcaaccac ctggtggacc tggagcggta tcccgagctc aagcggtcga
      481 tccaccacgg cgagacggtg ttcatccccg acgcgatgac cgacccgacg ctgaagcacg
      541 tgaagaacga gctgtcgagc cggcgcgtga agtcgatcac ggtggtgccc atcaattggc
      601 gcggtgtggc gatcggcgcg atcttcctgc gcacgttccg cgacggcccg accttctccg
      661 actccgacgt gcggttcgtg caggtcgtcg cctcgctcac cgcccaggcg ctgcgcaacg
      721 cccaccgcta cgagcgcctg gcccagcgcc aggccgaaac cggggagacg ctgcgccgca
      781 tggagctgga gcgcgtggcg ctgctgtcgt tcctgcggcg gctgatggag acgttcgggc
      841 gctccgacgg cgcctggggc gaagggctgc tgcccaaggc cagtgccgag gagctggacc
      901 ggctggtggg ggtcgcgctc gcggtgattc aggaagaagc caaaggccag tgaccgcgcc
      961 ggcggcgcgt gccgccgagc tccggcggca gctcgagcgg gccaaccacg cgtactacgt
     1021 cctcgaccgt cccgagattt ccgacgccga gtacgaccgg ctgttccagg agctccaggg
     1081 cctcgaggcg cgccatcccg agctccgcac ccccgactcc cccacccacc gtgtcggcgc
     1141 cgagccctcg ccggcgttca agaagcaccg ccacctcgtt cccatgctgt cgctcgccaa
     1201 tgcgttcgac gaagcgcagc tcacggcctg ggaggagcgc aacgcgcggc tcgtggccga
     1261 cgtcgcggag accggctaca cgctcgaggt gaagatcgac ggggccgccg tgagcctcac
     1321 ctacgccgac ggcgtgctcg tcaccggcgc cacccgcggc aacggcgtcg aaggcgagga
     1381 cgtgacgccc aacctgaaga cggttctcga cctcccgttg cggctccagg gaaagggctg
     1441 gcccacgcgg atggaagtgc ggggcgaggt gtacctctcc aaaagtcagt tcgagcgtgt
     1501 caacaaggag cgcgccaagg agggcgagcc gttgttcgcc aatccccgca acgcggcggc
     1561 gggcgcgttg cgccagctcg atccccggat cacccgagcc cggggcttgc gcgtcttctg
     1621 cttccacgtg gaagcgccac gacagacgct gccgtgcgac acccagcacg gcctgctgca
     1681 gcagctctcc acctggggct tcccggtgga gccgcatcac cagctcgtcc cgaatctcgc
     1741 cgccgcgcat cgcgccatcg ccaagctcga gaagaccctg ccgcagctgg actacggcgc
     1801 cgacggcgtg gtcgtgaagg tgaaccggct cgcgctgcac gcggagctcg gcacggtggg
     1861 cgaccgggag ccgcgctggg cgatcgcgcg caagttcgca cccgaggtcg ccgtcacgaa
     1921 actgctcgaa atccgcatca atgtcggccg cacgggggcg ctgaacccct acgccgtgct
     1981 ggaacccgtg gaggtggggg gcgtgaccgt ctccaccgcc accctgcaca acgccgacct
     2041 gatcgccgcc aaggacatcc gcatcggcga ctgggtggag gtgacgcgcg cgggcgaggt
     2101 gattccccag gtcctggggc cggtgcgggc aaagcgccgt gggaaggaaa agaagttccg
     2161 gatgccgaac cactgcccgg tgtgccgttc gcgcgtggag cggccgcagg acgaggtgat
     2221 gtcctactgc cccaacgtgt cctgcccggg acgcatctac gaggggatcg tccacttcgc
     2281 ctcgcgcggc gccatggaca tccgcggcct cggctacgag cgcgtcaagg cgctgctcga
     2341 cgcgaagctc atcgacgacg tgtccgatct gtacgatcgc aagcaggtca acctgctcaa
     2401 gctgctcacg ctcgagggct tcgccgaaaa gtcggcgcag cagctgctcg acgcgatcga
     2461 cgcctcgaag cagcggccgc tctccaccct gctgttcgcg ctgggcctcc gccacgtcgg
     2521 cgtgcagggc gcgcgcgttc tggcgcggca tttcggcacc atgagccggc tcgccgaggc
     2581 cacgcccgag gagatcggcg acgtgcgcgg ggtgggtccg accatcgccg aggccgtcgc
     2641 ggggttcttc gccgagccgc gcaaccgcaa gctcgtcgag cggctcgcga cgctcgggct
     2701 gaccatgacg gaaccgcgag cggcggcccg cacgggcggc gggcccctgg cgggccagac
     2761 ctacgtcatc accgggaccc tgccggagct g
//
LOCUS       DBNGZW010000546         2496 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_107244, whole genome shotgun sequence.
ACCESSION   DBNGZW010000546 DBNGZW010000000
VERSION     DBNGZW010000546.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2496)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_107244"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="AC16JC_13030"
     CDS             <1..975
                     /locus_tag="AC16JC_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5165666.1"
                     /translation="AWLVDALKATGFAEPMEGVFAKGQMMYGAAMLAGTLGGGLLGQI
                     DLAVPYLVRSATFLPLLGVAWFAMQEIGFTPRALEWRRVPVEMRRVFVEGLDYGLHHR
                     VVRPVMLASLVGMSFMIFGFYSWQRYFLDLLGRDLVWVSGLIAALLSIAMILGNALVR
                     PFSRFVRTRTGLLAGSVAVQAVTVVLCGLLGAGVTSGAIGGGVANIHGVPVRFIVVVA
                     LYLVYGIALGTMGPVKQGYLNAHIPSAQRATIISLDALFADAGGVVGQSGWGYLAKVR
                     SIGEAWMYAGASLLVGVPLYWAARRNDRQLDSFTDSHSDAQPHSGSQS"
     gene            complement(1035..1580)
                     /locus_tag="AC16JC_13035"
     CDS             complement(1035..1580)
                     /locus_tag="AC16JC_13035"
                     /inference="COORDINATES: protein motif:HMM:NF024102.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BsuPI-related putative proteinase inhibitor"
                     /protein_id="HIR5165667.1"
                     /translation="MHGRNALRTRRPTARSGARRACRCGTGLLTVAAAVLAVAETQSP
                     PPMPLERDAAESLQFTIVVPPLVHVAQPVRITLRLTNVTDHPVAVYVVGRPIAFDIVI
                     AREDGPVVWRRLEGAAVASILQVRTVRPRETMELSDLWPQRGSDGKPVPPGTYDVWGV
                     LPSDAPEPRRTATVKLRIMRK"
     gene            complement(1595..>2496)
                     /locus_tag="AC16JC_13040"
     CDS             complement(1595..>2496)
                     /locus_tag="AC16JC_13040"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chymotrypsin family serine protease"
                     /protein_id="HIR5165668.1"
                     /translation="DRWSQQVSDEAFMIAGVVYTDLDEAANRVTIGVEHAAAADSVRG
                     LAARLGIPAEALQVRLTEPIQFAATLRDQVRPVVAGLQINFGNYICSIGFNATSGGQS
                     SFVTASHCTNRQGGTEGTLYYQNLASTANSFIGTEVDDPAYFRGGPCPKGRKCRYSDA
                     ARAAYASGVTFTLGGIASTSGANNGSITITGTRSITGEGSALVGSVANKIGRTTGWTQ
                     GTVSATCVNTGVQGTNIVQLCQTFVDAGVGGGDSGSDVFANAGSSSATLLGILWGGNS
                     SGTMFVYSPIANVERELGPLTTF"
BASE COUNT          348 a          816 c          886 g          446 t
ORIGIN      
        1 gcctggctgg tggacgcgct caaggcgacg ggattcgccg agccgatgga gggcgtattc
       61 gccaagggcc agatgatgta cggcgcggcg atgctggcgg gcacactggg gggaggattg
      121 ctggggcaga tcgatctcgc cgtcccgtat ctcgtccgct cggccacctt cctgccgctg
      181 ctcggcgtcg cctggttcgc catgcaggag atcggcttca cccctcgggc gctcgagtgg
      241 cggcgggtcc ccgtcgaaat gcgccgggtg ttcgtggagg ggctggacta cgggctgcac
      301 caccgggtag tccgcccggt gatgctcgcg tccctggtgg gcatgagctt catgatcttc
      361 ggcttctaca gctggcagcg ctacttcctc gacctgctcg gccgggacct ggtgtgggtc
      421 tcgggcctga tcgccgcttt gctgtcgatc gccatgatcc tcggcaacgc cctggtgcgg
      481 ccgttcagcc ggttcgtccg cacgcggacg gggctgctcg cgggctcggt ggcggttcaa
      541 gcggtgacgg tcgtgctgtg cggactgctg ggcgccggcg tgacgtccgg agcgatcggg
      601 ggcggcgtcg cgaacatcca tggggtgccg gtacggttca tcgtcgtcgt ggcgctgtac
      661 ctcgtgtacg gcatcgcgct ggggacgatg ggtcccgtga agcagggata cctgaacgct
      721 cacatcccct ccgcccaacg ggccacgatc atctcgctcg atgcgctgtt cgcggacgcg
      781 gggggagtgg tgggacagtc ggggtgggga tacctggcga aggtcagatc catcggcgag
      841 gcgtggatgt acgcgggcgc ttcgctgctg gtgggggtgc cgttgtactg ggccgctcga
      901 cgcaacgaca ggcagttgga cagtttcaca gacagtcact cagacgctca gccgcatagc
      961 ggttcgcagt cctgagtcac tcagtcactg caacaccact acgcgcctga gcatctgagc
     1021 gcccgtctgt cctactactt cctcatgatc cgcaacttca cggtcgccgt cctccgcggc
     1081 tccggcgcat cgctcggcaa caccccccac acgtcgtagg tcccgggcgg gaccggcttt
     1141 ccgtcactcc cgcgttgcgg ccagaggtcg ctcagctcca tcgtctcccg cggccgcacc
     1201 gttcggactt gcaggatgct cgccaccgcg gccccttcga gccggcgcca cacgacgggc
     1261 ccgtcctccc gggcgatcac aatgtcgaag gcgatggggc ggcccacgac gtagaccgcg
     1321 acggggtgat cggtgacgtt ggtgaggcgc aacgtgatgc ggacgggctg cgcgacatgg
     1381 accagcggtg gcaccacgat cgtgaattgc agtgattcgg ctgcgtcgcg ctcgaggggc
     1441 atagggggcg ggctctgggt ctccgccacg gccagcacgg cggccgcgac ggtcaggagg
     1501 ccggtgccac agcggcaggc gcggcgcgct ccggagcggg ccgtgggtcg gcgggtccgg
     1561 agcgcgttgc gtccatgcat cggggaatag gcggtcagaa tgtcgtcagc ggtcccagct
     1621 ctcgctccac gttggcaatc gggctgtaca cgaacatggt gcccgacgag ttgccgcccc
     1681 agaggatgcc gagcagggtg gcgctcgagc tgccggcgtt ggcgaacacg tccgatccgc
     1741 tgtcgccgcc gcccacgccg gcgtccacga aggtctggca cagttgcacg atgttggttc
     1801 cctgcacgcc cgtgttcacg cacgtggcgc tgacggtgcc ctgggtccat ccggtggtac
     1861 gcccgatctt gttggctacg ctaccgacga gcgccgagcc ttctccggtg atggaacggg
     1921 tgccggtgat cgtgatcgac ccgttgttcg ccccactcgt gctggcgatc ccgccgagcg
     1981 tgaaggtcac accggaggcg tatgcggcac gcgccgcgtc gctgtaccgg catttcctcc
     2041 ccttcggaca ggggccgccg cggaagtacg cgggatcgtc cacctcggtg ccgatgaaac
     2101 tgttggccgt actggcgaga ttctgatagt agagggtgcc ctcggtgccg ccctgccggt
     2161 tggtgcagtg cgaggcagtg acgaacgagc tctgcccgcc gctcgtggcg ttgaagccga
     2221 tggagcagat gtagttgccg aaattgatct gcagcccggc gacgaccggc cgcacctggt
     2281 cgcgcagcgt ggcggcgaat tggatcggct ccgtcagccg cacctggagc gcttccgccg
     2341 ggatgccgag ccgtgcggcg agcccgcgga cgctgtccgc cgccgccgcg tgctcgacgc
     2401 ctatggtcac gcggttcgcc gcctcgtcga ggtcggtgta cacgactccg gcgatcatga
     2461 acgcctcgtc cgaaacctgt tgcgaccagc ggtcca
//
LOCUS       DBNGZW010000547         3027 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_998868, whole genome shotgun sequence.
ACCESSION   DBNGZW010000547 DBNGZW010000000
VERSION     DBNGZW010000547.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_998868"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..683
                     /locus_tag="AC16JC_13045"
     CDS             <1..683
                     /locus_tag="AC16JC_13045"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5165669.1"
                     /translation="QVATAMLVKRGHQVDVVANGRAAVDAVLARGYDVVLMDIQMPEM
                     DGFAATQRIRALPQGRTLPIIALTAHALSGERERCLTHGMNGYLAKPFKAHDLFSAVE
                     ERVTPPSSSLPVDLAAFRRVMREAGAEAAVDGIVAAFVESVPGHLAGLLAAARGREGE
                     PIAFAAHAFKSAAVTIGAQALAALLQSVEAAAREGDVARVRAALTDVEREARAALEYL
                     TTQGGTHA"
     gene            676..1257
                     /locus_tag="AC16JC_13050"
     CDS             676..1257
                     /locus_tag="AC16JC_13050"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5165670.1"
                     /translation="MPDAVPVLVVEDDPVSRAQFRALARAAGYQVNVAVNGREAWELF
                     QIARIPIVISDWYMPELDGPELCRRIRARPREPYVYFILVTAHGGKEQYLAGMEAGAD
                     DFIAKPVDPDELRARLTVAERILGLRHELRQLETLLPICSYCKRIRNERNEWETLDSY
                     LERRFETQLSHGICEECYARYVQPQLDRHPGPE"
     gene            complement(1286..1576)
                     /locus_tag="AC16JC_13055"
     CDS             complement(1286..1576)
                     /locus_tag="AC16JC_13055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165671.1"
                     /translation="MFVLVIDLSTARHAPDLLRSPCAYPASMVWVDPEEVESEPEDDE
                     RSRDSGVHRRGMRVFGPFETYEEGEYAATMLAADGDYRVIPVDPVATAELER"
     gene            complement(1592..1759)
                     /locus_tag="AC16JC_13060"
     CDS             complement(1592..1759)
                     /locus_tag="AC16JC_13060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165672.1"
                     /translation="MMKQAVGFLASVAGIGILALFVGVLVRLSWNTQTYPPRPTDRPD
                     PAQPEKVFREA"
     gene            complement(1970..2386)
                     /locus_tag="AC16JC_13065"
     CDS             complement(1970..2386)
                     /locus_tag="AC16JC_13065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5165673.1"
                     /translation="MSVDRPTAAPRYPHTAPALTVKRFDAPESVLVLDQGRLELITIG
                     GRLVGKGRYTPGWRLSHTLVPPRRAGTGPGEQTGVVLSGRAKVRAPGGVDIDLTPGDF
                     FHVAADYECWVVGYRPCEILYFDGMEALLDRMRERG"
     gene            complement(2798..>3027)
                     /locus_tag="AC16JC_13070"
     CDS             complement(2798..>3027)
                     /locus_tag="AC16JC_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017893521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HIR5165674.1"
                     /translation="IDDIVVVDARSRVYCDGIGCNASTKGAYRLPALGYRVKELLGGL
                     DFWLRDGHPVTRGEEAGQRPESGSGIACGCA"
BASE COUNT          497 a         1036 c         1002 g          492 t
ORIGIN      
        1 agcaggtggc gacagccatg ctcgtgaagc gcggtcatca agtggacgtc gtggccaacg
       61 gccgggcggc cgtcgacgcg gtgctcgccc gtgggtatga cgtcgttctc atggatatcc
      121 agatgcccga aatggatggg ttcgcagcca cccagcggat tcgcgccctg ccgcagggcc
      181 ggacgctgcc gatcatcgcg ctgaccgcgc acgcgctgag tggtgagcgc gagcgatgcc
      241 tgacccacgg gatgaacgga tatctcgcga agccgttcaa ggcgcatgac ctgttcagcg
      301 ccgtcgagga gcgggtgaca ccgccgtcgt cgtcccttcc ggtcgacctc gcggcgttcc
      361 gacgagtgat gcgggaggcc ggcgccgaag cagccgtgga cggcatcgtg gcggcattcg
      421 tcgaaagcgt gccgggacat ctcgcgggtc tgctcgccgc ggcccgggga agggaaggcg
      481 agccgatcgc gtttgccgcg cacgcgttca agtccgccgc ggtgaccatc ggcgcccagg
      541 ccctcgccgc gctgctgcag agcgtggagg cggccgctcg agaaggcgac gtcgcccgcg
      601 tgcgggcggc gctgacggac gtcgagcgtg aggcgcgcgc cgcactcgag tacttgacga
      661 cgcaaggggg gacacatgcc tgatgcggtc ccggtgctcg tcgtggagga cgacccggtg
      721 tcccgcgcgc aattccgcgc tctcgcccgg gccgccggct atcaggtcaa cgttgccgtc
      781 aacggccgtg aggcgtggga gctgttccag atcgcccgta tccctatcgt gatttcggac
      841 tggtatatgc cggagctgga cggcccggag ctgtgtcgcc ggatccgcgc ccgtcctcga
      901 gaaccctacg tctatttcat tctcgtcacc gcgcacggcg gcaaggagca atatctcgcc
      961 gggatggagg ccggcgccga cgacttcatc gccaaaccgg tcgatccgga cgagttacga
     1021 gcccgcctca cggtcgccga gcgtatcctt gggctgcggc acgagctgcg ccagctcgag
     1081 acgctgctcc cgatctgctc ttactgcaag cgcatccgca acgagcgcaa tgagtgggaa
     1141 acgctggaca gctacctgga gcggcgtttc gagacgcagc tcagtcacgg catctgcgag
     1201 gaatgctacg ccaggtacgt gcagcctcag ctcgaccgcc atccggggcc ggagtagcgc
     1261 gcgcgggggc ccggtttcct agcctttacc gctccagctc cgccgtcgcc accggatcga
     1321 ccgggatgac ccggtaatcg ccgtccgccg ccagcatcgt ggccgcgtac tcaccctcct
     1381 cgtacgtctc gaacgggccg aacacacgca tgccgcgccg gtgaaccccg gagtcgcggc
     1441 tccgctcgtc gtcctctggc tcgctctcca cttcttcagg gtccacccac accatgctcg
     1501 ccgggtatgc gcagggtgag cgaagcaggt ccggcgcgtg gcgcgcggtc gagaggtcaa
     1561 tcaccaagac gaacatgcat cccccttccg gtcacgcttc gcgaaagacc ttctccggct
     1621 gcgcaggatc tggtctgtcc gtggggcgag ggggataggt ctgcgtattc catgagaggc
     1681 gcacgagcac ccccacgaag agcgccagaa tgccgatccc ggcaaccgat gcgagaaacc
     1741 ccaccgcttg cttcatcatc ccgccacctc ttgttctggg cgctcactgg atccacctgg
     1801 taaagaacgc cctgcgccac gtaagtaacg cgggtggcaa gatttcccac tttcgcaccg
     1861 gaatgcgacc gaaacgtgat gccgaccacc gatcaggtcg taagctccat caccgcccga
     1921 cccacacggc tccgggctgt ggcgtgcgcg cgtcaggtgc ccaggtgcgc tacccccgct
     1981 cgcgcatgcg gtcgagcagc gcctccatcc cgtcgaagta caggatctcg cagggccggt
     2041 agccgaccac ccagcactcg tagtccgccg cgacgtggaa gaaatctccg ggggtgaggt
     2101 cgatgtcgac ccctccgggc gctcggacct tggcccggcc cgacaggacg acgccggtct
     2161 gctcccccgg cccggtgccg gcccggcggg gtggcacgag ggtgtgggac aagcgccatc
     2221 ccggcgtgta cctgcccttg cccacgagtc gcccgccgat ggtgatcagc tcgaggcgac
     2281 cctggtcgag caccagcacc gattccggcg cgtcgaagcg ctttacggtg agtgcggggg
     2341 cggtgtgcgg gtaacggggt gcggcagtcg gacgatcaac gctcatgcgg cctccttgcg
     2401 gcatcggctg aaggggcgtt tccgtgagcg tgtcgatgcc gtgccgcgcc ggcggaggga
     2461 gaaccgagtt cgggtcctct aaagggacta caccgggaac gggcgaacgg tcacatgggt
     2521 gcgccctttg ccctcgccgg acgagcgtat agaatacgga tgctcacgcc cgatccattc
     2581 actcaaccaa ggaggtgatc cattgtctag tcccagtggc ctgcgcaacc tggtatgggc
     2641 aagcgatcgc ctgatcggta gctgatccag gaccgaaccg cgatctgcgc ctcaggtcga
     2701 gcgcccatgg cgatcgcacc gggttgcgca gacaacaatg agccgcgtcg tcgaccgttc
     2761 ggtgtcggcg gcgcggctct gttttcacga actcgcgtca ggcgcagccg caggcgattc
     2821 cggacccgga ctcgggcctc tgtcccgctt cctcaccccg cgtcaccgga tggccgtccc
     2881 gtaaccagaa gtcgagtccc cccagcagct ccttcacccg atacccgagc gcggggagcc
     2941 ggtacgcccc tttggtcgac gcgttgcacc cgatgccgtc gcaatagacg cgggagcggg
     3001 cgtccacgac gacgatgtcg tcgatcc
//
LOCUS       DBNGZW010000548         4758 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1346680, whole genome shotgun sequence.
ACCESSION   DBNGZW010000548 DBNGZW010000000
VERSION     DBNGZW010000548.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1346680"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..522)
                     /locus_tag="AC16JC_13075"
     CDS             complement(<1..522)
                     /locus_tag="AC16JC_13075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165675.1"
                     /translation="MEIVGPAGAGKTALLRTLGRDPRVQAGVHIDRVRQFGELLVHGA
                     AILPLALSLLNQGWQRLWYGLLHFVRLRTLPRAIALAAAGAEADVILLDEGPVFSLAR
                     LSVFQQADQGDGRLSDAWHAELDRWSGLLDLVIWTDAPDAVLAERIRSRLKRHRVKAG
                     SDAEVVVFLERYRR"
     gene            complement(597..2141)
                     /locus_tag="AC16JC_13080"
     CDS             complement(597..2141)
                     /locus_tag="AC16JC_13080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HIR5165676.1"
                     /translation="MRPIVDPMTVAPPHPAAAAAASGPAATRLEILPRLFQRLDQEQI
                     RYCHWKSTEHLGATMTGATDVDVLVERSGAQRLTHLLTEGTTFKRFVVKAGRGYPGIE
                     DYVGFDAATGKLSHLHVHYQLTLGEKFLKGHRLPWETVVLDTRVRDEASGLWVTEPHH
                     ELLILIARHVMKLRLRDTLLAAVGHEYFRGGMLRELRWLTARVRSDELVKCAEPLVGQ
                     AAARMLPAMIAGRGPSITQLRSFRRHAVPRLARYRMYSGAGATLRMWLREWTWIWWRA
                     TNWLRRAPTRSTRTPPQGGLAIAFVGPDGSGKSTLTNAIAQWLSREVAVITTYGGSGK
                     GSASTSRRWLQRAGALTRPRKGSRDAEPSGARLLGKLLWVLALSRERRRWGAQVRRAR
                     NLGMVVLSDRLPQTQFAGLNDGPRYGQWLDSPSWLRRTVARREQATFREATIVPPDVI
                     VRLHVPIEVASQRKPDTPLDQLRRKIEIVAQLSFPPPTRVVEIDAAQAFDRVLQDVKR
                     ALWACL"
     gene            complement(2138..4114)
                     /gene="asnB"
                     /locus_tag="AC16JC_13085"
     CDS             complement(2138..4114)
                     /gene="asnB"
                     /locus_tag="AC16JC_13085"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013249226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR5165677.1"
                     /translation="MCGITGFWRPQGLHEEAARATLVEMTDTIRHRGPDDAGTWVDPA
                     AGIALGFRRLAIIDLTPTGRQPMTSGSGRFVMVFNGEIYNFQQLRRDLSALGTVFRGR
                     SDTEVLLEALEAWGLRRTLERCNGMFAFALWDKAERTLHLARDRAGEKPLYFGWSGGT
                     LLFGSELKALRAHPDFQPQIDRNALALYLRRGYVPGPYCIYRGIQKRRPGTWLTVRPD
                     RFGAGAVPTPYWSPRDVAEQAAEDPFRGSADEAVEALHELLTDAVKLRMEADVPLGAF
                     LSGGVDSTTIVALMQANSSRPVRTFTIGFREERHDEARYAAAIARHLGTEHTEQYLTP
                     AEARAVIPRLPVLYDEPFADTSQIPTFLVSELTRRHVTVSLSGDAGDELFGGYRRHRL
                     GPAIWRGISWIPLPVRRALSALLAPPRTARGGVLGQALSRLTHALTGKRNLVERVRQA
                     AEILPLESPIALYHYMMSYWKHPDILLPDAHELLVPATDPSRWPDLGASPQQIMMYLD
                     TVAWLPDDILVKLDRATMGTSLEARIPLLDHRVIEFAWRLPLSIRMRQKGTKWPLRQV
                     LARYVPPHLVDRPKRGFHMPVADWLRESLRDWAEALLDERHLRECGLDPRPVREKWAE
                     HLSGTTRWDYHLWTVLMYTAWVDSTTQTFHPAAA"
     gene            complement(4114..>4758)
                     /locus_tag="AC16JC_13090"
     CDS             complement(4114..>4758)
                     /locus_tag="AC16JC_13090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165678.1"
                     /translation="LADWLSREVAVVTTYGGSGKGSATLPRRLLQSVGALRRGLKRSS
                     RSSVAAVPAQIEAGVRRRATTPSLARAVWVLALARERRQRARDARRARGLGMIVLSDR
                     LPQTQFPGWNDGPRLAPWLETGPATLRSAARREQAAFQLAEMTPPDLVFKLHVSPAVA
                     ACRKPETPAEQLRTGIDMVRRLRFPPTTRVVELDAEQPLPTVLLAAKRALWDVI"
BASE COUNT          737 a         1640 c         1695 g          686 t
ORIGIN      
        1 ccggcgatag cgctccagaa acaccaccac ttccgcgtca ctgccggcct tgacccgatg
       61 gcgcttcagc cggctccgga tgcgctcggc gagcactgcg tccggggcgt cggtccagat
      121 gacgagatcg agcaggccgg accagcgatc cagttccgca tgccaggcat cggagagtcg
      181 tccgtcgccc tgatctgcct gctggaatac gctcagccgg gcgaggctga agaccggtcc
      241 ttcatcgagc aggatcacat cggcctccgc ccccgccgcg gcgagcgcga tcgcacgcgg
      301 caaggtgcgg agccgcacga aatgcagcaa cccgtaccac agccgttgcc acccctggtt
      361 caggagcgag agggcgaggg ggaggatcgc cgcgccgtgg accagcaact ccccgaattg
      421 gcgcacgcgg tcgatgtgga cgcctgcttg cacgcgaggg tcgcgcccga gcgtccgcaa
      481 cagggcggtc ttgccggcgc ccgcggggcc tacgatctcc acgatgagcg ggcgcgatcg
      541 ccgtgtcgag ccgccgacgg gcgtggaggc tgaggatcgg gagagcggcg tgcggctcac
      601 aggcaagccc acagggcccg cttgacgtct tgcaggaccc ggtcgaatgc ttgcgccgcg
      661 tcgatttcga cgacacgggt gggcggcggg aaggacagtt gggcgacgat ctcgatcttg
      721 cggcgcagct ggtccagcgg ggtgtccggc ttccgctgcg acgcgacctc gatcggcacg
      781 tgcagccgca cgatcacgtc gggcggaacg atcgtcgcct cccggaaggt cgcctgctcc
      841 cgtcgcgcga cggtgcgtcg cagccaggac ggggaatcga gccactgtcc gtaccggggt
      901 ccgtcgttga gtccggcgaa ctgggtctgc ggcagccggt ccgaaagcac gaccatgccg
      961 agattgcggg cgcgccgcac ctgggcgccc cagcgccggc gctcgcgtga cagcgcgagc
     1021 acccacagca acttgccgag cagtcgcgcg ccggacggct cggcatcgcg gctccccttt
     1081 cgtggccgag tcaacgcgcc cgcccgctgc agccagcggc gcgatgtaga ggcggagccc
     1141 ttgccgctgc cgccgtacgt cgtgatcacc gcgacctcgc gcgacagcca ctgcgcgatc
     1201 gcgttcgtga gcgtggattt ccccgaaccg tcgggaccga cgaaggcgat cgccaggccc
     1261 ccctgcggtg gagtgcgggt ggagcgcgtt ggggcgcgac gcagccagtt ggtggcgcgc
     1321 caccagatcc acgtccattc ccgcagccac atgcgcaggg tggccccggc gccggagtac
     1381 atgcggtagc gggcgagccg cggcaccgcg tggcggcgga acgagcggag ctgggtgatc
     1441 gaggggcctc ggccggcgat catcgccggc agcatgcgcg ccgccgcctg gccgacgagg
     1501 ggttccgcgc acttcaccag ttcgtccgag cgcacccggg cggtgagcca gcggagctcc
     1561 cgcagcatcc cgccgcggaa gtactcatga cccacggcgg ccagcagcgt gtcgcgcagc
     1621 cgcagcttca tcacgtggcg cgcgatgaga atgagcagtt cgtgatgcgg ctccgtgacc
     1681 cacaagccgg acgcctcgtc tcgcacgcgc gtgtcgagta cgaccgtttc ccaggggagc
     1741 cggtgacctt tgaggaattt ctctcccagc gtcagctggt aatgcacgtg caggtggctg
     1801 agctttcccg ttgcggcgtc gaacccgacg tagtcctcga tacccggata gccgcgtccc
     1861 gctttgacga cgaatcgttt gaaagtcgtc ccctcggtca acaggtgcgt gaggcgctgt
     1921 gccccgctgc gctccaccag cacatccaca tcggtcgcgc cggtcatggt cgctccgagg
     1981 tgctctgtgc tcttccagtg gcaatagcgg atctgctcct ggtcgagacg ctggaagagc
     2041 cgcggcagga tctccagacg ggtcgcggcc gggccggaag cggcggcggc cgcggcggga
     2101 tgaggagggg ccaccgtcat cggatcgacg atgggcctca cgccgccgcc gggtggaagg
     2161 tctgcgtggt gctgtccacc caggccgtgt acatgagcac ggtccagagg tgatagtccc
     2221 agcgcgtcgt tcccgacaga tgctcggccc acttctcccg gaccggccgc ggatcgagac
     2281 cgcactcgcg caggtgtcgc tcgtcgagca gtgcctcggc ccaatcccgc agcgattccc
     2341 gcagccagtc ggccaccggc atgtggaagc cccgtttggg ccggtccacc aggtgggggg
     2401 ggacgtagcg agcgaggacc tggcgcagcg gccacttggt gcccttctgg cgcatgcgga
     2461 tgctgagcgg caaccgccag gcgaactcga tgacccgatg gtcgagcaac gggatccggg
     2521 cctcgaggct cgtgcccatg gtggcgcgat cgagcttcac caggatgtcg tcgggcagcc
     2581 aggcgaccgt gtccaggtac atcatgatct gctgcgggct cgcgcccaga tcgggccagc
     2641 gcgacgggtc ggtcgcgggg acgagcagct cgtgggcgtc ggggagcaga atatcggggt
     2701 gcttccagta cgacatcatg taatggtaga gcgcaatcgg ggattccagc ggcagaatct
     2761 ccgcagcctg tcgcacccgc tcgaccaggt tccgcttccc ggtcagggcg tgcgtgagcc
     2821 ggctcagggc ctggcccaac acgccgcccc tcgcggtccg gggcggtgcc agcagggcgc
     2881 tcagtgcgcg tctgaccgga agcgggatcc agctgatgcc gcgccagatc gcgggcccca
     2941 gtcggtgccg gcggtagccg ccgaacagct cgtccccggc gtcccccgag aggctcacgg
     3001 tcacatgacg gcgcgtgagc tccgacacga ggaaggtagg gatctgcgaa gtgtcggcga
     3061 acggttcgtc atagagcacc ggcagacggg gaatgacggc gcgcgcttca gccggcgtca
     3121 ggtactgctc ggtgtgctcg gtgccgaggt gccgggcgat tgccgccgcg taccgggcct
     3181 cgtcgtggcg ttcctcgcgg aatccgatgg tgaacgtgcg caccgggcgg gagctgttcg
     3241 cctgcatgag cgccacgatc gtggtggaat cgaccccgcc cgagagaaac gcgccgaggg
     3301 gcacgtccgc ctccatgcgc aacttgaccg catcggtcag gagctcgtgc agcgcctcca
     3361 ccgcttcgtc ggcgctgccg cggaacgggt cctccgcggc ctgctcggcc acgtcgcgcg
     3421 gcgaccaata gggggtcggc accgcccccg cgccgaagcg atcgggccgc accgtcagcc
     3481 aggtgcccgg ccgccgcttc tggatgccgc gatagatgca gtagggaccg gggacgtacc
     3541 cgcgccgcag gtagagcgcg agtgcgttcc gatcgatctg gggttggaag tcggggtgtg
     3601 cccggagcgc cttgagctcc gagccgaaca gcagcgtgcc tcccgaccag ccgaaataca
     3661 acggcttctc accggcgcgg tcgcgggcca gatgaagcgt gcgctcggcc ttgtcccaga
     3721 gcgcgaacgc gaacatccca ttgcagcgct ccagcgtgcg ccgcagcccc cacgcctcca
     3781 gggcctccag cagcacttcc gtatccgacc gtccgcggaa caccgtcccg agcgcgctca
     3841 gatcgcggcg caactgctgg aaattgtaga tctcaccgtt gaataccatc acaaagcgcc
     3901 cggaccccga tgtcatgggc tgccggccgg taggggtgag atcgatgatg gccagccgtc
     3961 ggaaaccgag cgcgatgccc gccgccgggt cgacccaggt ccccgcgtcg tccgggccgc
     4021 ggtgccggat cgtgtcggtc atctcgacga gcgtggcgcg ggctgcctct tcgtggagcc
     4081 cctgcggtcg ccagaagccg gtgatgccgc acatcagatc acgtcccaga gcgcccgctt
     4141 cgccgccagc agcaccgtgg gaagcggctg ctcggcgtcg agctccacga cccgcgtggt
     4201 gggcgggaac cggagccggc ggaccatgtc gatcccggtg cgcagctgct cggcgggcgt
     4261 ctcaggcttg cggcatgcgg cgaccgcagg cgagacgtgc agcttgaaca cgaggtccgg
     4321 cggcgtcatc tccgccaact ggaacgcggc ctgttcccgg cgcgccgcgg accgcagggt
     4381 tgcaggaccg gtctcgagcc agggagcgag ccgtggaccg tcgttccagc cggggaactg
     4441 cgtctggggc agccggtcgg agaggacaat catgcccagc cccctggcgc gccgggcatc
     4501 ccgggctcgc tggcgtcgct cacgcgccag cgcgagcacc cacacagcgc gcgccaagga
     4561 gggcgtcgtc gcccgccggc gtacccccgc ctcgatttgg gcgggcacgg ccgcgaccga
     4621 cgaccggctg gatcgcttca ggccacggcg cagggcaccc acggactgca gcaggcgacg
     4681 gggcagggtt gcggagccct tgccgctgcc gccataggtg gtgactacgg cgacttcgcg
     4741 tgacaaccag tcggcgag
//
LOCUS       DBNGZW010000549         2697 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_447581, whole genome shotgun sequence.
ACCESSION   DBNGZW010000549 DBNGZW010000000
VERSION     DBNGZW010000549.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_447581"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..172)
                     /locus_tag="AC16JC_13095"
     CDS             complement(<1..172)
                     /locus_tag="AC16JC_13095"
                     /inference="COORDINATES: protein motif:HMM:NF013225.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HIR5165679.1"
                     /translation="MLRGDALGTATLLGGGPKRLKAQHDKGKLSARERLDVLLDEGSF
                     VEIDRFVTARSAE"
     gene            101..1606
                     /locus_tag="AC16JC_13100"
     CDS             101..1606
                     /locus_tag="AC16JC_13100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020403313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR5165680.1"
                     /translation="MLRLEPLRAAAEQGGGPERVATQHKKGKMTARERLDVLLDPGSF
                     TELDRFVVHRSTDFGLAEQQVPGDGVVTGWGRIEGRLVYVFAQDFTVFGGSLSETHAE
                     KICKVMDLAVRNGAPIIGLNDSGGARIQEGVASLGGYADIFLRNTLASGVVPQISAVL
                     GPCAGGAVYSPAITDFICMVRGISYMFVTGPNVVKTVTHEEVDFEQLGGADVHGATSG
                     VAHFVYDSELECLTALRTLLSYLPSNNLESPPVRGSADPPDRRDEALLDIVPDEANKP
                     YDMHDVIRRVVDGGEFLEVHADYATNLVVGFARLAGRSVGIVANQPASLAGVLDISAS
                     LKGARFIRFCDAFNIPLVTFEDVPGFLPGTDQEFHGIIQHGAKLLYAFAEATVPKVTV
                     IARKAYGGAYCVMASKHIRTDFNYAWPTAEIAVMGERGAVEILFKGEATEERVAEYRT
                     KFAHPYLAAGRGFLDDVIDPRDTRPRLIEALTSLESKRDRNPPKKHGNIPL"
     gene            1671..>2697
                     /locus_tag="AC16JC_13105"
     CDS             1671..>2697
                     /locus_tag="AC16JC_13105"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR5165681.1"
                     /translation="MTLSRILVANRGEIALRIIRACHEEGFEAVAVYSDADRLSPHVR
                     AANDAVRLGPPPAAESYLNIGKLIDAAQRTGCHAIHPGYGFLSERATFAQAVTDAGLV
                     FVGPSASAIRAMGDKTEARRRMQHAGVPVVPGIARALRDAADAALEAERIGFPVLLKA
                     SAGGGGKGMRVVQGAAEMPGAFQAARSEAVKAFGEGSLYLEQYVERPRHVEIQVLADM
                     HGRVVSLGERECSIQRRHQKLIEEAPSVAVTPGLRRRMGEAATAAAGAVGYCSAGTVE
                     FLLAPDGEFYFLEMNTRIQVEHPVTELVYGVDLVREQLRIAAGQPMRVAAGLLQPRGH
                     AIECRITA"
BASE COUNT          412 a          904 c          969 g          412 t
ORIGIN      
        1 cttccgccga gcgcgcggtg acgaaccggt cgatctcgac gaagctgccc tcgtcgagca
       61 gaacgtcgag gcgctcgcgc gcggacagct ttcccttgtc gtgctgcgcc ttgagccgct
      121 tcgggccgcc gccgagcagg gtggcggtcc cgagcgcgtc gccacgcagc acaagaaggg
      181 caagatgacg gcgcgcgagc gcctcgacgt gctgctcgat cccggctcct tcacggagct
      241 cgaccggttc gtcgtccatc gatcgaccga tttcgggctc gccgagcagc aggtgccggg
      301 ggacggcgtc gtcaccggct gggggcgcat cgaggggcgc ctggtgtacg tattcgccca
      361 ggacttcacc gtcttcgggg gctcgctctc ggagacgcac gccgagaaga tctgcaaggt
      421 gatggacctc gcggtgcgca acggcgcccc catcatcggc ctgaacgatt cgggcggcgc
      481 gcgcatccag gagggggtcg cttcgctggg agggtacgcc gacattttcc tgcgcaacac
      541 gctcgcctcg ggcgtggtcc cgcagatcag cgcggtgctc ggcccctgcg cgggaggggc
      601 ggtgtacagt cccgcgatca ccgacttcat ctgtatggtg cggggcatct cctacatgtt
      661 cgtgaccggc cccaacgtcg tgaagacggt gacgcacgag gaggtggatt tcgagcagct
      721 gggtggcgcc gacgtgcacg gcgcgacgtc gggtgtcgcg cacttcgtgt acgattcgga
      781 gctcgagtgc ctgacggcgc tccgcacgct gctctcctac ctgccgtcga acaacctcga
      841 gagcccgccg gtgcgcggct cggcagaccc gccggaccgg cgggacgaag ccctgctgga
      901 tatcgtcccc gacgaggcga acaagccgta cgacatgcac gacgtgatcc gtcgcgtggt
      961 ggatgggggc gaattcctgg aggtgcacgc cgactacgcg accaacctcg tggtcggctt
     1021 cgcgcgactg gcggggcgtt cggtggggat cgtggcgaac cagccggcga gcctcgccgg
     1081 cgtcctcgac atcagtgcgt cgctcaaggg cgcccggttc atccgcttct gcgatgcctt
     1141 caacatcccg ctcgtgacgt tcgaggacgt ccccggcttc ctcccgggaa ccgaccagga
     1201 gttccacggg atcatccagc acggcgccaa gctcctctac gcgttcgccg aggcgacggt
     1261 gccgaaggtc accgtcatcg cgcgcaaggc gtacggcggg gcctactgcg tgatggcgag
     1321 caagcacatc cgcaccgact tcaactacgc ctggcccacc gcggagatcg cggtcatggg
     1381 cgagcgcggc gccgtggaga tcctgttcaa gggggaggcg accgaggagc gggtggccga
     1441 gtaccgcacc aagttcgccc acccgtatct cgcggccggt cgcggcttcc tggacgacgt
     1501 gatcgacccg cgggacacgc gtccgcgtct catcgaggcg ctgacctcgc tggagtccaa
     1561 gcgggaccgg aatccgccga agaagcatgg gaatatcccg ttgtgagagg cgtcgtcagt
     1621 cggcggtcgt cggtcatcgg tcaggaccga acaccgacga ccgataaccg atgacgctct
     1681 ctcgaattct cgtcgccaac cgcggcgaga tcgcgctgcg gatcatccgc gcctgtcacg
     1741 aagaaggatt cgaggcggtc gcggtctatt ccgacgccga ccggctctcc ccgcacgtgc
     1801 gcgccgcgaa cgacgcggtg cgattgggac cgccgccggc ggcggagtcc tacctcaaca
     1861 tcggcaagct gatcgacgcc gcgcagcgca ccgggtgcca cgcgatccac ccagggtacg
     1921 ggttcctgtc cgaacgggcc accttcgccc aggccgtcac cgacgcggga ctggtgttcg
     1981 tgggaccctc cgcgtccgcg atccgcgcga tgggcgacaa gaccgaggca cggcgccgca
     2041 tgcagcacgc cggggtgccc gtggtgccgg gaatcgcccg cgcgctgcgc gatgcggcgg
     2101 acgcggcact cgaggccgag cgcatcgggt ttcccgtcct gttgaaggcc tcggcggggg
     2161 gtggtgggaa ggggatgcgg gtggtgcagg gcgcggccga gatgcccggc gcgtttcagg
     2221 cggcgcgaag cgaggccgtc aaggccttcg gcgaggggag cctctacctc gagcagtatg
     2281 tcgagcgtcc gcggcacgtc gagatccagg tgctcgccga catgcacggg cgcgtggtgt
     2341 cgctgggcga gcgggaatgc agcatccagc gccgccacca gaagctcatc gaggaggcgc
     2401 cctccgtggc ggtcacgccg ggattgcggc ggcgcatggg ggaggcggcg acggcggcgg
     2461 cgggcgccgt cggttattgc agcgcgggca cggtcgagtt cctcctggcg cccgacggtg
     2521 agttctactt cctggagatg aacacgcgca tccaggtcga gcatcccgtg acggagctcg
     2581 tctacggcgt cgatctggtg cgcgagcagc tgcgcatcgc cgcggggcag ccgatgcggg
     2641 tggccgccgg cctgctgcaa ccccgcggcc acgcgatcga atgccgcatc acggccg
//
LOCUS       DBNGZW010000550         4652 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1551937, whole genome shotgun sequence.
ACCESSION   DBNGZW010000550 DBNGZW010000000
VERSION     DBNGZW010000550.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1551937"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2380
                     /locus_tag="AC16JC_13110"
     CDS             <1..2380
                     /locus_tag="AC16JC_13110"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5165682.1"
                     /translation="VGHTHREMRDSVINGVHFVQPRNWALSLSVVHVWLARDSTAPGR
                     WRVAQVRGDLIPLGDVGEVPQFVRRFADAHERVRAWADEALGTGGPGFDGRYGRAADT
                     PLLDFVNAVQRRRSGAQLAATADFDPSSGLPDGTIHRRDVAGIYPYENTLVALRISGR
                     QLRDFLEHSAEYYRTLAPGRRVVNDAVQGYNFDVVSGASYRIDLTRPPGQRIVSLTVN
                     GRAVSAADSFTFATNSYRAAGGGGYAMLLGAPIVYDRGEDIGELLAEEVRRVDTVTAA
                     AYYTPSWAILPPGDSAARAAFAPPPPPVPQTDSTLLRVLAITDFHGALQPRVWAWSQG
                     RPVGGAAAIKTWLDNLAKDCGCTSVRLDGGDELQGTPLANFEFGRPVIAAFNSLAIDA
                     AAIGNRDFDWGVDTLQARVREARYPFVSANISDSAGRVPDWVSPWTVVTRGGAKVAVI
                     GLTTRSTPETTRPGNVAGLVFGDLAGAVRRVLPAARAAADFVIVLAHDGEMCDSAGCR
                     GEILTLAASLDSASVDLILAGHTHERVDTVVHGIPIVQAGSSGRVIAVVDFVRAAGGG
                     RVVRTRLETPWADSVTPDRDITTALFRDQHVVDSITTRTVARLRTALGRAGDEYPLGR
                     LVADAYRNIGKADVALINNGGIGADLPAGLVSYGALFDVQPFQNRLVRVTLTGAELRE
                     VLEHALAGGRPGVHVSGLQVWYSPSRPEGRRIRRIRLLNGRSLDRKRHYTLAVPDFLA
                     AGGDGFAMLTTAPRVDAGVVDIDAVIAYLGVVPQPVSAPGDVRFHGDERETR"
     gene            2377..3642
                     /gene="add"
                     /locus_tag="AC16JC_13115"
     CDS             2377..3642
                     /gene="add"
                     /locus_tag="AC16JC_13115"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIR5165683.1"
                     /translation="MTADSVRVFVNERPVDVPRGATVKDAVAALDRSLADLLSADGGG
                     GGAYVTDGVGRTVDAGDPIPEAGAVLRVVVTARRGPPRLTKEVLRTWPKAELHVHLDG
                     CLRPRTMLELARAQGVRLPADTPEDLARALMITGAQSLEEYLERYAVNLSVMQTAEAL
                     ERIAYEFVVDVAQENVRYVEVRYSPLLHQPALTLSRAIEAPLAGVRRASAETGTKVGL
                     ILCGIRTLPPARSLDLARAAADYRGEGVVAFDLAGAERGHSARDFRTAFEFAAQHGLA
                     CTCHAGEGDGPHSIGDALHVCAAQRIGHGTRLGEDPALLEEVIARRIPLEMCLTSNVH
                     THTVASLREHPFRTYLQRGVVVTLNTDGRLVDGVTLTDEYFTAHAVLGLTREELARVT
                     LNACESAFLPEFERVALVSRVQSELEKLA"
     gene            3669..4481
                     /locus_tag="AC16JC_13120"
     CDS             3669..4481
                     /locus_tag="AC16JC_13120"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012010450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR5165684.1"
                     /translation="MIEEAARAVSKRLEGRAPTVAIVLGSGLGHLAGEVKQAVRLPYA
                     EIPGFPQPSVAGHKGELVAGTLEDMPVLVQSGRFHLYEGHSADVAGLPVRVFARLGVR
                     TLIVTNAAGGVRPTFRAGTLMLIADHVNLMFRNPLIGPVLPGEERFPDMSEPYDTGLR
                     ALAREAAREAGIALEEGVYVGLLGPSYETPAEIRMLQRLGIDAVGMSTVPEVIVARAR
                     GIRCLGISTITNLAAGISPTKLSHAEVLDVGRQVGQALATIVRGVVRLLPGA"
BASE COUNT          650 a         1589 c         1715 g          698 t
ORIGIN      
        1 ggtgggccac acccatcgcg aaatgcgcga ctcggtgatc aacggcgtcc atttcgtgca
       61 gccgaggaac tgggcgctga gcctgtcggt ggtgcacgtc tggctggccc gggacagcac
      121 cgcgccgggg cggtggcggg tcgcccaggt gcgcggcgat ctcattccgc tcggcgacgt
      181 gggcgaggtt ccccagttcg tgcgccgctt cgcggacgcg cacgagcggg tgcgggcctg
      241 ggccgacgag gcgctcggca ccggggggcc cggcttcgac gggcgatacg gccgcgccgc
      301 ggatacgccg ctgctcgatt tcgtcaacgc cgtgcagcga cgccggtcgg gtgcccagct
      361 cgcggccacc gcggacttcg atccgtcgag cgggttgccc gacgggacga tccaccggcg
      421 cgacgtcgcg gggatctacc cgtacgagaa caccctcgtc gcgctgcgca tctcgggacg
      481 gcagctcagg gacttcctgg agcattcggc cgagtattac cggaccctcg cgccgggacg
      541 ccgcgtcgtg aacgatgccg tgcagggcta caacttcgac gtggtgagtg gggcgagcta
      601 ccgcatcgac ctgacgcgac ccccggggca gcgcatcgtg agcctcacgg tgaatggccg
      661 tgccgtgtcc gccgccgaca gcttcacctt cgccaccaac agctaccggg ccgccggcgg
      721 aggcgggtac gcgatgctgc tcggcgcgcc gatcgtgtac gatcgtggcg aggatatcgg
      781 tgagctgctg gccgaggagg tccgccgggt cgatacggtc accgctgccg cctactacac
      841 gcccagctgg gccatcctgc ctcccggcga ctccgcggcg cgggctgcgt tcgcgccgcc
      901 cccgccgccg gttccccaga ccgacagtac cctgctgcgc gtcctggcga tcacggactt
      961 ccacggtgcg ctgcagccgc gcgtgtgggc ctggtcgcaa ggccggccgg tcggaggcgc
     1021 cgcggcgatc aagacctggc tcgacaacct cgcgaaggac tgcggctgca cgagcgtccg
     1081 cctcgacggc ggcgatgagc tccagggcac gccgctggcg aacttcgaat tcggccggcc
     1141 ggtgatcgcg gccttcaact cgctggcgat cgacgccgcc gcgatcggca accgcgactt
     1201 cgattggggt gtcgatacgc tgcaggcgcg cgtgcgcgaa gcccggtatc cgttcgtatc
     1261 cgccaacatc agcgactccg ccgggcgcgt gccggactgg gtgtcaccgt ggaccgtggt
     1321 gacgcgcggc ggcgcgaagg tggcggtcat cggcctgacc acccgcagca cgcccgaaac
     1381 gactcgtccc ggcaacgtgg ccgggctggt gttcggcgac ctggcgggcg ccgtgcgtcg
     1441 ggtgctgccc gccgcgcgcg cggcggcgga cttcgtcatc gtactcgcgc acgacggcga
     1501 gatgtgcgat tcggccggct gccggggcga gatcctcacg ctcgccgcga gcctcgattc
     1561 ggcgtcggtg gacctgatcc tcgccggaca cacgcacgag cgggtggata cggtggtgca
     1621 cggcatcccg atcgtgcagg cggggagctc ggggcgggtc atcgcggtgg tggatttcgt
     1681 ccgcgccgcc gggggggggc gtgtagttcg gacgcgactc gagacgccgt gggcggactc
     1741 ggtcaccccg gatcgcgaca tcaccaccgc actgttccgc gatcaacacg tggtggacag
     1801 catcacgacc cggacggtgg cgaggttgcg cacggcactg ggtcgggccg gggacgagta
     1861 cccgctgggg cgactcgtgg cggacgccta ccggaacatc gggaaggccg acgtcgccct
     1921 gatcaacaac ggcggaatcg gcgccgactt gcccgcgggt ctcgtcagct atggcgcgct
     1981 gttcgacgtg cagccgttcc agaaccgcct ggtccgggtc accctgacgg gcgccgagct
     2041 gcgcgaggtc ctggagcatg cgctcgccgg gggcaggccc ggggtccacg tgtccggatt
     2101 gcaggtctgg tacagcccct cccggcccga aggccgtcgc attcgcagga tccgactgct
     2161 gaacggccgg tcgctcgacc ggaagcgaca ctacacgctg gcggtccccg atttcctcgc
     2221 ggccggcggc gacggcttcg cgatgctcac gacggccccg cgcgtcgacg ccggggtggt
     2281 ggacatcgac gccgtcatcg cgtatctggg cgtggtgcca cagccggtga gcgcccccgg
     2341 cgacgtgcga ttccatgggg acgagaggga gacgcggtga ccgccgacag cgtacgggtc
     2401 ttcgtcaacg agcggccggt ggacgtcccg cggggcgcga ccgtgaagga cgcggtcgcg
     2461 gcgctcgacc gatcgctggc ggacctgctc tccgcggacg gcggaggggg gggcgcctac
     2521 gtcaccgatg gggtggggcg gacggtcgat gccggggatc cgatccccga agcgggcgcg
     2581 gtgctgcggg tcgttgtcac ggcccgccgc ggaccgccgc gactgacaaa ggaagtgctg
     2641 cgcacctggc ccaaggccga gctgcacgta catctcgacg gctgcctgcg gccgcgcacc
     2701 atgctggagc tggcgcgggc ccagggcgtg cgcctcccgg ccgacacgcc cgaggatctg
     2761 gcgcgcgcgt tgatgatcac cggcgcgcag agcctcgagg agtacctcga gcgatatgcc
     2821 gtgaacctgt cggtgatgca gacggccgag gccctggagc ggatcgcgta cgagttcgtg
     2881 gtggacgtcg cgcaggagaa cgtgcgctac gtggaagtcc gctactcccc gctgttgcac
     2941 cagccggcgc tcacgctcag ccgggcgatc gaggcgccgc tggcgggcgt gcggcgggca
     3001 tcggcggaaa cgggcacgaa ggtggggctc attctgtgcg gcatccgcac gctgccgccg
     3061 gcgcgctcgc tcgacctggc ccgggcggcg gccgactacc gcggcgaagg cgtggtggcc
     3121 ttcgatctgg ccggcgccga acgcgggcat tccgcgcggg acttccgtac cgcctttgaa
     3181 tttgccgccc agcacggcct ggcgtgcacc tgtcatgcgg gcgaaggcga cggccctcat
     3241 tcgatcggcg acgcgctcca cgtgtgcgcg gcacagcgga tcggccacgg cacccggctc
     3301 ggcgaggatc cggcgctgct cgaggaggtc atcgcccgcc ggattccact ggagatgtgt
     3361 ctgacgagca acgtccacac gcacacggtg gcctcgctgc gcgagcaccc gttccggacc
     3421 tacctgcagc ggggcgtcgt cgtcacgctg aataccgacg gccggctggt ggacggcgtc
     3481 accctcaccg acgagtattt caccgcgcac gccgtcctcg ggctcacccg ggaagagctg
     3541 gcgcgggtca ccctcaatgc ctgtgagagc gccttcctgc cggagttcga gcgggtagcg
     3601 ctggtgtcac gggtccaaag cgaactggag aagctcgcgt gagcgacaca tcgggtgcgc
     3661 gtggtgccgt gatcgaggaa gccgcgaggg cggtgagcaa gcggctcgag ggccgcgcgc
     3721 cgacggtcgc gatcgtgctg ggctccgggc tggggcacct ggccggcgaa gtcaaacagg
     3781 cggtgcgcct tccctacgcg gagatccccg gattcccgca gccgagcgtc gcggggcaca
     3841 aaggcgagct ggtcgccggg acgctggagg atatgccggt cctcgtgcag agcggccggt
     3901 tccacctgta cgaaggccac tccgcggacg tcgccgggct tccggtacgc gtattcgccc
     3961 ggctgggcgt ccgcacgctg atcgtcacca acgcggccgg tggagtgcgt cccacgttcc
     4021 gtgcgggtac gctgatgctg atcgcggatc atgtgaatct gatgttccgc aatccgctga
     4081 tcggccccgt gctgccgggc gaagagcggt tcccggatat gagcgagccg tacgacacgg
     4141 ggttgcgcgc gctggcacgg gaagccgccc gggaggccgg cattgcgctg gaggaggggg
     4201 tgtacgtcgg gctgctcggc ccctcgtacg agacgccggc ggaaatccgt atgctgcaac
     4261 gacttggaat cgacgccgtg ggcatgtcta cggtgcccga agtgatcgtg gcccgcgcgc
     4321 gcggcatccg gtgtctcggg atctcgacca tcacgaatct cgcggcgggg atctccccca
     4381 ccaaactgtc ccacgccgaa gtgctcgacg tcggtcgtca ggtgggccag gcgctggcca
     4441 cgatcgtgcg cggcgtggtg cggctgctcc ccggcgcctg acgccgccgg accgggcgac
     4501 gcccggccgc tgcaaccttt tttctccgac cgacgtccaa gggttcgatc cgcgcgcgcc
     4561 gtcacgcgcc tcgagtcgcg ctgtccaacc ctgcagccgg tcgggcgtat gacgacgatc
     4621 tcgtgtttcc tgttgtgtct cgcagcggca gc
//
LOCUS       DBNGZW010000551         6147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1810690, whole genome shotgun sequence.
ACCESSION   DBNGZW010000551 DBNGZW010000000
VERSION     DBNGZW010000551.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6147
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1810690"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1195
                     /locus_tag="AC16JC_13125"
     CDS             <1..1195
                     /locus_tag="AC16JC_13125"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR5165685.1"
                     /translation="NPVFISRLDGHEALANTAAIRAAGVTRATPTPAGGEILHDARTA
                     EPTGIFKDQALGLIGKAIPAPSAEEMDSAVARAMAYAASLGVTATANMSASWADLASY
                     RRLERAGRMTLRVYLYLPIDDWRTVADTVRATGSGDEWVRIGGLKGFMDGSAGSRTAY
                     FFDAYDDSAGYHGLLQNPEADMRTWIGNADSAGLQVAVHAIGDRANAILLAVYDSVAR
                     VHGARDRRFRVEHAQHLRPEDIPRFGRLRIVPSMQPYHAIDDGRWVEQRIGPERIKTT
                     YAFRTLLETEAPLAFGSDWTVATLDPLWGLYAAVTRRTLDGKHPEGWVPDQKITLAQA
                     LRAYTAGNAWAVFAENQWGSLAPGRDADVVVLDRNLFTTPPESLATARVAVTIVGGKV
                     VYRKS"
     gene            complement(1227..1709)
                     /locus_tag="AC16JC_13130"
     CDS             complement(1227..1709)
                     /locus_tag="AC16JC_13130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR5165686.1"
                     /translation="MDDSAKKTILRRFPYGLYAVTVAHGGEEHGMTANWVTQAAFDPP
                     MVVVAVENTSKTIGMIRDAHYFVINLLAAGQRELAGRLGRSSEQAPHKLKGLKTKPAP
                     VSQTPVLAEAIGWVECRAVASLPAGDHTLFLGAIVEAGVENADGQPLTLAEAGFKYAG
                     "
     gene            1796..2992
                     /locus_tag="AC16JC_13135"
     CDS             1796..2992
                     /locus_tag="AC16JC_13135"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5165687.1"
                     /translation="MHRRLAVVTVLALVCPPRLAAQDDKPVPLADLEAAAARDSLDAE
                     TLYRLALRYDLMNRYDDAERVARGAVSIDPRCARAWLLLAYLPYDRRPKLWEEVRKGK
                     VPPAWQAAIAESDRLERRAFLIDPLVDFRVLGAPPPKQDVVAIPDYGEYTTAYLLLLG
                     LSEFGWRYELSYSAFDLYARRRYKGLPIDSIPSGVFWWRGLAAAHLGSYTRAVADIQV
                     LLDRSLKHEAGDSLIQMDLGTNDFRYLLALFKQRLHRPADAMTLYQQALASDLGLYMA
                     HVRLAQLYAENHMWAQAVEESRRAVMANPDDPTLLVDLAEMLRDGGRLVEAEEAFRQA
                     AQANARNPRVSYELGLVEQQVGNPSAAKEAFARFVAIAPSGMSTEIADAKRRLAALPV
                     STGNQE"
     gene            2998..4188
                     /locus_tag="AC16JC_13140"
     CDS             2998..4188
                     /locus_tag="AC16JC_13140"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5165688.1"
                     /translation="MKRFRAAGILLATLAVTPVAAQRIKLAASLADLEKAARKDSNDA
                     AAHYNVALAYWNAKRWDDVDSALQRAVRLEPRFAAAYMALAELPYARRPQLLEEEFEH
                     RVPDDWRSRIDESERRYRQAVLIDPLVEVRIGYVMLPTQGQFQTSLAVLFGDWIDDYV
                     EGEDMYFGGRYQEAYDRFQRVYNEIKAGTHSNRLWNTLLYWHGLSAAQIQKHDDAIWD
                     FGAILDRFENAEERARDSTLHVPLRSNEFRYVLGVMKQRAGQYNEAIDLYRKALENDI
                     GLYMAHVRLAQIYEDHNMLAQAVAERRAAVNANPDDPSLLLDLGKTLARANQWAVAEQ
                     ALQDAVAANPRDPRPHYFLGIVEQHQNKTPEARTAFTDFIALAPTRYALQVADARQRL
                     DALH"
     gene            4217..5755
                     /locus_tag="AC16JC_13145"
     CDS             4217..5755
                     /locus_tag="AC16JC_13145"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR5165689.1"
                     /translation="MALLLVATAASSLPAQQTSAYEELQAFSGVLNYIRLNYPDTVAY
                     SKLVHAAIDGVLHALDPHSAFYSRLEFERRTALERGELAVTGLAVEEVDSAVTVLAVA
                     ARGPAAKAGVQPGDRLVSINDTAVAGLTVRSVELRLAGEKGSKVRLQFERGPRLEPDT
                     FRVTVKRDFIPFRSVSLVRMVDSTTGYVRLEEFGPEAAKEVHEALDRLRKQHMRRAIL
                     DLRGNPGGLVVSAVELASEFFPRGTVVFRTRGRKRDVDTTYTTRRDGDYVALPLVVLI
                     DENSASAAEALSASLQDHDRALLLGRRSFGKALMQMDFVVMPMGDDLHLTIGYVLSPS
                     GRYIQRRYRGLAVEQYLAFAGKSGAESDTLQEYHTDAGRVVRGGGGIAPDIALSRPTP
                     LPVWWSVAADSGFNEAVSDSVALTLPATPAARAAWLGSPDRWRAAVLPPFLVRARARL
                     HVTALTDSTLDARLARILAARVAEVRWGPDALEEFLVRSSPDVQAAVEYFPRLPGLLA
                     APRH"
     gene            5771..>6147
                     /locus_tag="AC16JC_13150"
     CDS             5771..>6147
                     /locus_tag="AC16JC_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5165690.1"
                     /translation="MTTPPRTRARGEAQSAAPRQARPPLSKADLLDVYRLMLLSRRLD
                     DKEIQLKRQNKIFFQISGAGHEAVLVAAGKALRAGYDWFYPYYRDRGLCLALGMTPTE
                     MLLSAVAAADDPNSGGRQMPSHWG"
BASE COUNT          978 a         2160 c         2015 g          994 t
ORIGIN      
        1 caaccccgtc ttcatcagcc ggctcgacgg acatgaagcc ctcgccaaca cggccgccat
       61 ccgggcggcc ggggtcacca gggccacgcc cacgccggcg ggaggcgaga tcctgcacga
      121 cgcccggacc gctgagccca ccggcatctt caaggaccag gccctggggc tgatcggcaa
      181 ggcgatcccc gcaccgtcgg ccgaagagat ggactccgcg gttgcccgcg ccatggcgta
      241 cgcggcctcg ctcggcgtca cggcgacggc caacatgtcc gcctcttggg cggacctcgc
      301 gagctatcgg cggctggagc gcgccgggcg gatgaccctg cgtgtgtatc tgtatctccc
      361 gatcgacgac tggcgcaccg tcgccgacac cgtgcgcgcc accggctcgg gcgacgagtg
      421 ggtgcgtatc ggcgggttga agggattcat ggacggctcg gccgggtcgc ggacggccta
      481 tttcttcgac gcgtacgacg attccgcggg ttaccatggg ctgctgcaga atcccgaagc
      541 cgacatgcgg acgtggatcg ggaacgcgga ctccgccggc ctccaggttg cggtgcacgc
      601 catcggggat cgcgccaacg ccatcctgct cgccgtctac gacagcgtcg cccgggtgca
      661 tggcgcccgc gaccgtcgct tccgcgtcga gcacgcccag cacctccgcc ccgaggatat
      721 cccgcggttc ggtcgcctgc gcatcgtccc gtcgatgcag ccctaccacg cgatcgacga
      781 cggccgctgg gtggagcagc ggatcggccc ggagcgcatc aagaccacgt acgcctttcg
      841 gaccctgctc gagacggagg cgccgctcgc cttcggctcc gactggaccg tcgccacgct
      901 cgatccgctg tggggcctct acgcggcggt gacccgtcgg acgctcgacg gcaagcatcc
      961 cgagggctgg gtgccggacc agaagatcac gctggcgcag gccctgcgtg cctatacggc
     1021 cggcaatgca tgggcggtgt tcgcggagaa ccagtggggg agccttgccc ccggacgtga
     1081 tgccgacgtc gtcgtcttgg atcgcaacct gttcaccacg ccgcctgaat ccctggcgac
     1141 ggcgagggtg gcggtcacca tcgtgggggg aaaagtagtc taccggaaaa gctgatagct
     1201 gatagcagac agccgatagc cctcacctac cccgcgtact tgaaccccgc ctcggccagc
     1261 gtcagcggct ggccgtcggc attctccacg cccgcctcca cgatcgcacc cagaaacagc
     1321 gtatggtcac ccgccgggag cgaggcgacc gcgcggcact ccacccaccc gattgcctca
     1381 gccaggacgg gagtctgcga gacgggagca ggcttcgtct tgagcccctt gagcttgtgc
     1441 ggggcctgct cggaggagcg ccccagcctg cccgccagct cccgctgccc ggccgcgagg
     1501 aggttgatga cgaaatagtg cgcgtcgcgg atcatcccga tcgtcttcga ggtgttctcg
     1561 acggccacta cgaccatcgg cggatcgaag gccgcctggg tgacccagtt ggcggtcatg
     1621 ccgtgctctt caccgccgtg cgcgacggtc actgcgtaca ggccgtacgg aaagcggcgc
     1681 aggatggtct tcttggcaga atcgtccata tgaggaatat tgacgttgcc gggcgccttt
     1741 ggccaatgtg gtacggatca cagcgattca ccgtcgactg cggtaactgg gcactatgca
     1801 tcgccgtctg gccgtcgtca ccgtcctcgc gctcgtctgt cctccgcggc ttgcggcaca
     1861 ggacgacaag ccggtccccc tcgcggacct cgaagccgcg gccgctcgtg attcgctcga
     1921 cgccgaaacg ttgtatcgtc tggcgctgcg ctacgacctc atgaaccggt acgacgacgc
     1981 cgaacgtgtg gcccgtgggg cggtgagcat cgatccgcgc tgcgcgcggg cgtggctcct
     2041 gctcgcctac ctgccgtacg accgcaggcc gaagctgtgg gaggaggtcc gcaagggaaa
     2101 ggtgccgccc gcctggcagg cggcgattgc cgaatccgat cggctggagc gccgcgcctt
     2161 tctcatcgac cccctcgtgg atttccgcgt gctgggagcg ccgccgccga aacaggacgt
     2221 cgtcgccatc cccgactacg gggaatacac caccgcgtac ctcctgctgc tcggcttgag
     2281 cgagttcggc tggcgctacg agctgtccta cagtgcgttc gatctctatg cccgacgccg
     2341 ctacaagggc ctgccgatcg attccatccc cagcggcgtc ttctggtggc gcggcctcgc
     2401 cgcggcccat ctcggcagct acaccagggc tgtcgccgat atccaggtcc tgctcgatcg
     2461 ctcgctgaag cacgaggccg gcgattcgct gatccagatg gatctcggga ccaacgactt
     2521 ccgctacctg ctggccctgt tcaagcagcg gctgcaccgg cccgccgacg ccatgacgct
     2581 gtaccagcag gccctggcct ccgacctcgg actctatatg gcccacgtgc ggctggctca
     2641 gctgtacgcg gagaatcaca tgtgggccca ggcagtggag gaatcccgcc gcgcggtgat
     2701 ggcgaacccg gacgacccca ccttgctcgt agacctggcg gagatgctcc gggacggcgg
     2761 ccggctggtg gaagccgagg aggcgttccg gcaggccgcg caggcgaacg cgcgcaaccc
     2821 cagagtctcc tacgagctcg ggctcgtgga gcagcaggtg gggaatccat ccgcggcgaa
     2881 ggaggcgttc gcgcgcttcg tggccatcgc gccgagcggc atgagcacgg agattgcgga
     2941 tgcaaaacgg cgcctggcgg cactcccggt atccaccggt aaccaggagt gaatccgatg
     3001 aagcggttcc gcgctgctgg aatcctgctc gccacgctcg ccgtcacccc cgtggcggcg
     3061 cagcgcatca agcttgccgc gagtcttgcc gacctggaga aggcggctcg caaggactcg
     3121 aacgatgccg ccgcccatta caacgtcgcg ctcgcctact ggaacgcgaa acgatgggac
     3181 gacgtggatt ccgcgctcca acgggcggtc cggctcgaac cccgtttcgc cgcggcctac
     3241 atggcgttgg ccgagcttcc ctatgcgcgg cgcccccagc tactggagga ggagttcgag
     3301 caccgcgtcc ctgacgattg gcgctccagg atcgacgagt cggaacgccg ctatcggcag
     3361 gccgtactca tcgatccgct ggtcgaggtc cgcatcgggt acgtgatgct gccaacccag
     3421 ggccaatttc agacctcgct cgccgtgttg ttcggagact ggatcgacga ctatgtcgaa
     3481 ggcgaggaca tgtatttcgg gggccggtat caggaggcct acgatcgctt ccagcgggtc
     3541 tacaacgaga tcaaggcggg gacccacagt aaccggctct ggaatactct cctgtactgg
     3601 catggtcttt cagccgcgca gatccagaag cacgacgacg cgatctggga tttcggcgcc
     3661 atactcgacc gattcgaaaa cgcggaagag cgggccaggg actcgaccct gcacgtcccg
     3721 ctccgcagca acgagtttcg ctacgtgctc ggcgtgatga aacagcgtgc aggacagtac
     3781 aatgaagcga tcgacctcta ccgcaaggct ctggagaacg acatcggcct ctacatggcc
     3841 cacgtccgtc tggcgcagat ctacgaggac cacaacatgc tggcacaggc ggtggccgag
     3901 cggcgcgcgg cggtgaatgc gaaccccgac gatcccagcc tgcttctcga tctcggcaag
     3961 acgctcgccc gcgccaacca gtgggccgtt gcggagcagg ccctgcagga cgccgtcgcc
     4021 gccaatcccc gcgatccccg gccgcactat ttcctcggca tcgtggagca gcaccagaac
     4081 aagacgccgg aggccaggac cgcgttcacg gacttcatcg ccctggcccc cacccgctat
     4141 gctctgcagg tcgcggacgc caggcagcgg ctcgatgcgc tccactgagc cgcggcggta
     4201 cggtgccgct ggcggagtgg ccctgctcct agtcgccacg gccgcctcgt ctcttcccgc
     4261 tcagcagacc tccgcgtacg aggagctgca ggcgttcagc ggcgtcctca actacatccg
     4321 gctcaactac cccgacacgg tggcgtactc gaagctcgtg cacgcggcga tcgacggcgt
     4381 gctgcacgcc ctcgacccgc acagcgcttt ctactcacgc ctcgagttcg agcgccgcac
     4441 ggcgctcgag cgcggcgagc tggccgtgac cggcctcgcc gtcgaggagg tggacagcgc
     4501 cgtcacggtc ctggcggttg cggcgcgcgg tcccgccgcc aaagccggcg tccagcccgg
     4561 cgaccgtctg gtatcgatca acgacaccgc cgtagccgga ctgacggtcc gcagcgtcga
     4621 gctgcgactc gccggtgaaa agggatccaa ggtccgcctg cagttcgagc gcggcccccg
     4681 cctggagccc gacacgttcc gggtcaccgt gaagcgggat ttcatcccgt tccggtcggt
     4741 ctcgctcgtc cgcatggtgg attccaccac cggttacgtc cgtctcgagg aattcggacc
     4801 ggaggcggcc aaggaagtgc acgaggcgct cgaccggctg cggaagcaac acatgcgccg
     4861 ggcgattctc gacctgcggg gcaaccccgg tggcctggtc gtatcggcgg tcgagctggc
     4921 atcggagttc ttcccgcgcg ggacggtcgt gttccgcacg cgcgggcgca agcgcgatgt
     4981 ggacacgacg tataccacca gacgcgacgg cgactacgtg gcgctgccgc tcgtggtgct
     5041 gatcgacgag aacagcgcca gcgccgcgga ggcgctctcc gcctcgctcc aggatcacga
     5101 ccgggcgctc ctcctgggcc gtcgcagctt cggcaaggcg ttgatgcaga tggacttcgt
     5161 cgtcatgccg atgggggatg acctgcacct cacgatcggc tacgtcctct cgcccagcgg
     5221 ccgctacatc cagcggcgtt atcgcggtct cgcggtcgag cagtacctgg ccttcgccgg
     5281 caagtccggc gccgaatcgg atacgctcca ggagtaccat accgacgccg ggcgcgtcgt
     5341 gcgcggcggc ggcggcatcg cccccgatat cgcgctttcg aggcccacac cgcttcccgt
     5401 ctggtggagt gtcgccgccg attccggctt caacgaagcc gtgtccgaca gcgtcgcgct
     5461 gacactcccg gcgacgcccg ccgcccgggc cgcctggctc gggtcgcccg accggtggcg
     5521 ggctgcggtg ttgcccccgt tcctggtgcg cgcccgcgcc cggctccacg tgacggcgct
     5581 gaccgacagc accctcgacg cgcggctcgc gcgcatcctc gccgcgcggg tcgcagaggt
     5641 tcgctggggg ccggatgcac tggaggagtt tctcgtgcgc tcgagtccgg acgtgcaggc
     5701 cgccgtggag tatttcccgc gccttcccgg gcttttggcg gcgccgcgtc actaggctat
     5761 gttttgaccc atgacaacgc ccccccgcac gcgcgcgcgc ggtgaagcgc agtccgctgc
     5821 cccccgccag gcccgcccac cgctctccaa ggccgatctg ctcgacgttt atcggctgat
     5881 gctcctgtcg cggcgtctcg acgacaagga aatccagctc aagcggcaaa acaagatctt
     5941 ctttcagatc tcgggggccg gccacgaggc ggtcctggtg gccgcgggga aggctctgag
     6001 ggcgggatac gactggttct atccctacta ccgcgatcgc ggactctgtc tcgcgcttgg
     6061 catgaccccc accgagatgc tgctctcggc cgtggccgcg gccgacgatc ccaattcggg
     6121 cgggcgccag atgccctcgc attgggg
//
LOCUS       DBNGZW010000552         2306 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_323257, whole genome shotgun sequence.
ACCESSION   DBNGZW010000552 DBNGZW010000000
VERSION     DBNGZW010000552.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2306)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2306)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2306
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_323257"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="AC16JC_13155"
     CDS             complement(<1..947)
                     /locus_tag="AC16JC_13155"
                     /inference="COORDINATES: protein motif:HMM:NF024977.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5165691.1"
                     /translation="MAGVSLVDDRSALLSMRNSDFDAYSAVGEVIDNSIQAEAQNVRL
                     IVAFSADRHEPITSIAFGDDGTGMSGDVLHRCLQLGYSTRYNDRRGIGRFGVGATLAA
                     ISQCRKIEVSSREERGQWLYTYIDLDAITADPPTMKEIPPPTQRAVPEALRALVGPAS
                     GTLVVWSKCDRQPAPASKLLEELRVWIGRTYRKFIWDGVRIFINGDIVKAIDPLYVTT
                     ARTKFPRDPKAHEYEEMLLPWPVPPADRQDGAPAESIVRIRMSLLPEALRPAEESGHG
                     PAAKERCIDLNEGVSILRNKREVFYDVIPWWPGIPFRGI"
     gene            1128..>2306
                     /locus_tag="AC16JC_13160"
     CDS             1128..>2306
                     /locus_tag="AC16JC_13160"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5165692.1"
                     /translation="MLSDYERLAEALADRYRLERPLGQGGMATVHLAEDLKHRRKVAV
                     KVLGSDYSSVVMAARFSREIEIAAALNHPNILAVHDSGQAAGLLYYVMPYVEGQSLRD
                     RLRRETRLPLEEALRIAKEVADALGYAHSQGVIHRDIKPENILLQSGHAVVADFGIAR
                     AVTSDEGTRGAVLTQPGAPIGTPDYMSPEQAAGERELDGRCDQYALACVLYEMLAGQP
                     PFTGPTVESVLIRHLTSEVPRITDLQPDVPKPVAAALTRALAKTPAERFSGMAAFVEG
                     LAAPDAPAGAPPPAQLTSFIGRERETAIVQELLLDTRLLTLTGAGGSGKTLLALEVAN
                     RVRARYPDGVAWVELASLSNPELVPHHVADALRVRRDGIRSAEDALLEALRDRDALLV
                     L"
BASE COUNT          376 a          786 c          781 g          363 t
ORIGIN      
        1 tcgattcccc gaaacgggat gcccggccac cacgggatga cgtcgtaaaa gacttcacgc
       61 ttgttccgca gaatcgacac cccttcgttc agatcgatgc accgctcttt cgcggcagga
      121 ccgtggccgc tctcttcagc gggccgaagc gcttcgggca agagactcat acggatgcgt
      181 acgatcgact ctgccggggc tccgtcctga cggtccgcgg gcgggaccgg ccagggcaac
      241 agcatctcct cgtactcgtg cgccttgggg tcgcggggaa acttcgttcg ggccgttgtg
      301 acgtagagcg ggtcgatggc cttgacgatg tcaccgttga tgaagatgcg gacgccgtcc
      361 cagatgaact tgcgatacgt cctcccgatc cagaccctca gctcttccag cagcttcgag
      421 gcgggagccg gctggcggtc gcacttgctc cacacgacga gcgtgccgct ggccggcccc
      481 acgagggctc ggagcgcttc ggggacggcc cgctgcgtcg ggggagggat ttccttcatc
      541 gtgggagggt ctgccgtgat ggcatccaga tcgatgtagg tgtacagcca ctgcccgcgc
      601 tcctccctgg aggagacttc gatcttcctg cattgactga tggccgccag ggtcgcaccg
      661 accccgaagc gtccgatgcc gcgccggtcg ttgtagcggg tggagtagcc caactggaga
      721 cagcgatgca gaacgtcgcc ggacatgccc gtaccgtcat cgccaaaggc aatcgaggtg
      781 atcggttcgt gccggtcggc gctgaaagcg acgatcaacc ggacgttttg tgcctcggcc
      841 tgaatggagt tgtcgatcac ctcgccgacg gcactgtacg catcgaaatc cgaattcctc
      901 atggagagga gtgccgagcg gtcatcaacg agactcacgc ccgccatagc agcctccttt
      961 cagcgcacga tccgacagtc ccctttcagc acacgatcag gtggtgagat ctcccagaca
     1021 gcccggaatc gtctagtcgc aatgcgtaca acacgccggt ccgtagcgca agcaggaagg
     1081 cctcggggcc ggtgccgggg cctcgtgcgc cgacgcatat tccgccgatg ctctcggact
     1141 acgagcgcct cgccgaggcg ctggccgacc gctaccgcct ggagcgcccc ctgggtcagg
     1201 gggggatggc cacggtgcac ctcgccgagg acctgaaaca ccggcgcaag gtggccgtca
     1261 aggtactcgg ctccgattac agctcggtgg tcatggccgc ccgcttttcg cgcgagatcg
     1321 agatcgccgc cgcgctcaat caccccaaca tcctcgccgt gcacgactcc gggcaggccg
     1381 ccggcctcct ttattacgtg atgccctacg tcgaggggca gtcgttgcgc gaccgcctgc
     1441 ggcgcgagac gcggctgccg ctcgaggaag cgctgcgcat cgcgaaggag gtggcggacg
     1501 cgctgggcta cgcccatagc cagggggtga ttcatcgcga catcaagccg gagaacatcc
     1561 tgctgcagtc gggacacgcc gtggtggcgg acttcggcat cgcccgcgcg gtcacgagcg
     1621 acgaagggac gcgcggcgcg gtgctgacgc agcccggcgc gccgatcggc acgccggact
     1681 acatgagccc ggagcaggcc gcgggcgaac gggagctgga cgggcgctgc gatcaatacg
     1741 cgctggcgtg cgtgctctac gaaatgctgg cggggcagcc gccgttcacc gggccgacgg
     1801 tcgagagcgt gctgatccgg cacctgacct ccgaagtgcc gcggatcacg gatctgcaac
     1861 cggatgtccc caagccggtg gccgccgcgc tgacccgtgc cctggccaag acgccggccg
     1921 agcggttcag cgggatggcg gccttcgtcg aggggctggc agcaccagac gccccggctg
     1981 gcgcgccgcc gcccgcccaa ctcacctcct tcatcggccg cgagcgcgag acggcgatcg
     2041 tccaggagct gctgctggac acgcggctgc tgacgctcac cggcgccggc gggagcggaa
     2101 agaccctgct cgccctggag gtggcgaacc gcgtgcgggc gcgctacccc gacggcgtcg
     2161 cctgggtgga gctcgcgtcc ctctcgaatc ccgagctcgt cccgcaccac gtggccgacg
     2221 cgctgcgcgt gcggcgcgac ggcatccgct cggccgagga cgccctgctc gaggcgctgc
     2281 gcgaccggga cgcactgctc gtgctg
//
LOCUS       DBNGZW010000553         5722 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1346898, whole genome shotgun sequence.
ACCESSION   DBNGZW010000553 DBNGZW010000000
VERSION     DBNGZW010000553.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1346898"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2220)
                     /locus_tag="AC16JC_13165"
     CDS             complement(<1..2220)
                     /locus_tag="AC16JC_13165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5165693.1"
                     /translation="MDALVQDLRYALRTLARAPGFTAVAVLTLALGIGANTAIFSVLY
                     GVLLRPLPYPDADRLVQFAQTYQGGSGLMDVTYREFQFLQQNSTAFQSLTATTSVGFN
                     LFSGTEAQRVSGLRVSHQYFRVLGVAPQLGRQFLPEEDQSGGASAAILSYGLWQRRFG
                     GDPDVLGRVISLDGVPTTVVGVMPAGFHALPPVDIWSTLAQVAQTIGSGQNLDLIARL
                     KPGVSLQQAQAVIQPTTAAYRAAFSQIVSPDVGIELASYQKLVSLDLQTPVRLVFGAI
                     ALVLLIACANVASLVLARAAARNRELAVRVALGASGGRLTRQLLTESVLLAFVGGATG
                     LLFAAWGLRTLLSLAPPGLTSTAEIHLDRWALIFTFAVSLATGVVFGLVPAREAARSH
                     LHDALREGSGRATSAHHRSRLRSALVVGEVALSLVLLAGGALLVRTFANLLRSDTGFE
                     PGHVLSAEIWLTGSRYDSTAAIAGFYGELTRRLESLPGVEQAAVVEAGLPLVRGGNLP
                     VTVDGEFHSTDYRTVTPGYFATLRIPLVAGRVLEAGDAEHAEPVVVVNRSFAHRYMAD
                     SALGRIIKVGGSDQNPYRRIVGVVGDVKSFVGFPAPPTVFITSSQTPAGFTRIFGSWF
                     PTHVLVRATGDPRMLRTILARVIHETDAQVPVGQVRSMPEVLAASLASQRFIMMLLTV
                     FAGLAVVLAAVGLYGVLSYLVGQRTHEIGVRVALGARPHQVLALVLARGLVLAGTGVA
                     "
     gene            complement(2302..4698)
                     /locus_tag="AC16JC_13170"
     CDS             complement(2302..4698)
                     /locus_tag="AC16JC_13170"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5165694.1"
                     /translation="MDALLQDLRYALRTLAKAPGFALVAIVTLGLGIGATTAVFSVVQ
                     GLLLRSLPFPHAERLVDIKLVRAESRHAGMIGGATSPLASYRTWRTATAAFEDMAAYT
                     GDDAVLTGMGPAERVVAWHVSANFLPLLGARPLLGRGFAVDEDRPGSAPVAILSQAFW
                     TTRFGGDRAVLGRAITLDTVVYTIVGVMPSRFRYPADADVWSNLGSALSGPSGARRAH
                     EWGFWVVGRLRPGGSLARAQDQLDAATRSAWSAEPDLAGTLPVVGALQGYLVRDVRAS
                     LWILLGATTLVLLVACANVSGLLLARGSDREREMSIRVPLGAESRRLARLVVSESSLL
                     AAGGGLLGMLLALWIVPTVVALAGPELPVIQGFGVDVRALGAALVLSIVAAVLAALAP
                     ALRIALRPPSDALRGARPGTLRTWRSLPANTLVVAQVAISIVLLAGAGLLVRSFDRMV
                     HTSPGFDATDVVVAELHLPPHRYATGAARRAYLARALDRAKSLPGAAAVVPTTGIPLS
                     GGEIGSVSKPGTPARPDQPLAWVSAVTPDYFRILRIPALRGRLPEAVHEAAIDAAAAR
                     TYFAGQDPLGRNLTVGGHAELTVVGIVGDTRQESLGESPPPPHVYPVLPAEPGPYLKL
                     LVRAAGDPLPLAAALRRELQAVDVDVPLDRVTPLSTLMSASLATQRLYTWLLGGFSVV
                     ALLLAGTGLYGLVSHGVIRRTREIGIRMALGANRRAVVALVVGHGLMLTTAGLVAGLG
                     GTVAATRVLRRYLFEVTPTDPVVLTAVAGVLLCAAPAAAYVPSRRATRVDPMVALRTE
                     "
     gene            complement(4780..>5722)
                     /locus_tag="AC16JC_13175"
     CDS             complement(4780..>5722)
                     /locus_tag="AC16JC_13175"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HIR5165695.1"
                     /translation="LPGVAAVAIAANHPANAGFTSSIAVVGREAEAGDWPEPAIRIVT
                     STYFGTLGVPLLAGRYLEATDAVDAPTVVVINEAARRRFFPTQDPLGQRIRLWGGERL
                     VVGVVGNERFRGLMAATPPGLYLPLAQVPVADGGHTVLVRVHGDLGAFTPALRRVVAD
                     LDPELPLFGVEPLAATLSNTVAQQRFTMIVLVLFAAVALLLAVIGIYGLLSYTVTQRT
                     REIGIRIALGAEPRRVRDLVVGQGAMLAGAGLVLGLAGALALTRVLTTMLYGVGARDP
                     LTLVGVALTLGLVALLASDVAARRAARLDPMVALRTE"
BASE COUNT          979 a         2009 c         2032 g          702 t
ORIGIN      
        1 cgcgacaccc gtccccgcca ggaccaggcc gcgcgcgagc acgagagcca gaacctggtg
       61 cggccgcgcc cccagcgcca cgcgcactcc aatctcgtgg gtgcgttggc cgaccaggta
      121 ggacaacacg ccatacagcc cgaccgcggc cagcaccacc gccaaaccag cgaacacggt
      181 gagcagcatc atgatgaacc gctgagatgc gagcgaagcg gcgagcacct cgggcatcga
      241 gcggacctgc cccaccggga cctgggcatc ggtctcatga atgacgcgag cgagaatggt
      301 ccgcagcatc cgtggatccc ccgtcgcacg gacgaggaca tgcgtgggga accagctgcc
      361 gaagatgcgc gtgaacccgg ccggcgtctg tgacgaggtg atgaagaccg tcggcggggc
      421 gggaaaaccg acgaacgatt tgacgtcgcc aacgaccccg acgatgcggc gatacggatt
      481 ctggtcgctg ccgccgacct tgatgatccg tcccagcgcg gaatcggcca tataccgatg
      541 cgcgaacgac cgattcacga ccacgaccgg ctcggcgtgc tccgcgtcgc cggcttccag
      601 cacgcgcccg gcgacgagcg gaatccgcag ggtcgcgaag tagcccgggg tgacggtgcg
      661 gtagtccgtg gagtggaatt cgccgtccac cgtgacgggc aggttgccgc cgcgcacgag
      721 cggcaacccc gcttcgacca cggccgcctg ctccacgccg ggaagggact cgagccgccg
      781 ggtcagctcg ccgtagaagc ccgcaatggc cgccgtggag tcgtagcgag acccggtcag
      841 ccagatctcc gccgacagca cgtggccggg ctcgaacccg gtgtcgctgc gcaacaggtt
      901 ggcgaacgtt cgcaccagca gcgccccacc cgccagcagc acgagggaga gcgcgacctc
      961 gcccacgacc agcgcactgc gcaaccggct gcggtggtgg gcgctggtgg cgcgcccgga
     1021 accctcccgc agcgcgtcgt gcaggtggga acgggcggcc tcgcgagccg gcacgaggcc
     1081 gaacacgacc ccggtggcga gcgagaccgc gaaggtaaag atcagggccc agcggtcgag
     1141 atggatctcc gcggtgctcg tgagcccggg cggcgcgagg gagaggaggg tgcgcagtcc
     1201 ccacgcggcg aacagcaacc cggtcgcgcc accgacgaag gccaggagca cactctcggt
     1261 gagcagctgc cgcgtgagcc gaccaccgct cgcgcccagg gcgacgcgca ccgccagctc
     1321 gcggttgcgg gctgcggctc gtgccagcac caggctcgcg acgttggcgc aggcgatcag
     1381 caggacgagg gcgatggctc cgaacacgag gcgaaccggc gtctggaggt ccagggagac
     1441 gagcttctga taggacgcga gctcgatccc cacgtcgggt gacacgatct ggctgaatgc
     1501 ggcccggtag gccgccgtcg tcggctgaat caccgcctgc gcctgttgca gggagacgcc
     1561 gggcttcagg cgagcgatga ggtcgaggtt ctggccgctg ccaatggtct gggccacctg
     1621 tgccagcgtg gaccagatgt ccaccggggg cagcgcgtgg aatccggccg gcatcactcc
     1681 caccacggtg gtggggacgc catcgagcga gatcactcgg cccaggacgt cgggatcacc
     1741 gccgaagcgg cgctgccaca ggccgtagct gagaatggcg gcgcttgcgc cgccgctctg
     1801 gtcttcctcg gggaggaact ggcggcccag ctggggagcc acgcccagaa cgcgaaagta
     1861 ctgatgggag acgcgcaggc cgctcacgcg ctgcgcctcc gtcccggaga acagattgaa
     1921 accaacggac gtcgtcgccg tgagggactg aaacgccgtg ctgttctgct gcaggaactg
     1981 gaactcccgg taggtcacgt ccatcaggcc gctgccgccc tggtaagtct gcgcgaactg
     2041 gacgagccga tcggcgtcgg ggtagggcaa ggggcgcaac agtacgccgt agagcacgct
     2101 gaagatcgcg gtgttggccc cgatgccgag tgcgagagtg agcacggcga cggcggtgaa
     2161 gccgggcgcc ctggcgaggg tgcggagggc gtagcggagg tcttgcacga gagcgtccat
     2221 tgtgtctcca tgcaggtgcc aggttccagg tgtctggtgt cagggtgaac cacctgacat
     2281 ctggcccctg gcacccggca cctattcagt tctcagcgcc accatcggat ccacccgcgt
     2341 cgcccgccgc gatgggacgt acgcggccgc cggcgcggcg cacaacagga ctcccgcgac
     2401 cgccgtcagc accacgggat cggtgggcgt gacctcgaag aggtagcgcc gcaggacgcg
     2461 cgtggccgcg acggttcccc cgagcccggc cacgaggccc gcggtggtca gcatgaggcc
     2521 gtgtcccacg accagcgcga ccaccgcccg ccggttcgcg ccgagagcca tgcgaatgcc
     2581 gatttcacgc gtgcggcgga tcaccccgtg cgataccaaa ccgtacaacc ccgtgcccgc
     2641 gagcagcagc gcgacgacgg agaagccccc gagcagccac gtgtagagcc gctgagttgc
     2701 cagggatgcg ctcatcagcg tggacaatgg cgtcacgcgg tccaggggga cgtctacgtc
     2761 caccgcctgc agctcgcgcc gcagcgcggc ggcgagcggg agggggtctc ctgcggcacg
     2821 gacgaggagc ttcaggtacg ggcctggctc ggcgggcaac acggggtaga cgtgcggtgg
     2881 cggtggcgat tcgccgagcg actcctgccg ggtgtcgccg acgattccca cgactgtgag
     2941 ctccgcgtgg ccgcctacgg tcagattccg accgagcgga tcctgacccg cgaagtacgt
     3001 ccgcgccgcc gcggcgtcga tcgccgcctc gtgcacggct tccggaagac gcccgcgcag
     3061 tgccggaatc ctgaggattc ggaaataatc cggagtgacg gcgctcaccc acgccagggg
     3121 ttgatcgggg cgggccggag tccccggctt cgagacggag ccgatttccc cgccggacag
     3181 cgggatgccg gtggtcggaa ccacggcggc cgcgccggga agggatttcg cccgatcgag
     3241 cgcgcgtgcc aggtatgccc gtcgcgctgc acctgtcgca tagcggtgcg gcggcagatg
     3301 caactccgcg acgacgacgt cagtggcgtc gaagcccggg ctggtatgga ccatgcggtc
     3361 gaagctccgg accagcagcc cggcgccggc gaggaggacg atggaaatcg cgacctgcgc
     3421 gacgaccagg gtgttcgccg gcagactccg ccatgtgcgc agcgtgccgg gtctcgcgcc
     3481 cctcagcgca tcgctgggag gccgcagggc gatccgcagc gccggggcga gtgcggcgag
     3541 gaccgccgcc acgatactca gcacgagggc cgctcccagc gctcggacgt ccactccgaa
     3601 tccctgaatg acgggcaact cgggcccggc gagcgccacc acggtcggca cgatccagag
     3661 ggcgaggagc atcccgagaa ggccgccgcc ggcggcgagc agcgaggatt cgctgacgac
     3721 cagccgggcg aggcgcctgc tctccgcgcc cagggggacg cggatactca tctcccgctc
     3781 gcggtccgag ccgcgcgcga ggagcagccc cgacacgttg gcgcaggcga ccagcaggac
     3841 cagcgtcgtc gcgccgagca ggatccagag cgacgcgcgc acgtccctta cgaggtagcc
     3901 ctgcaacgcc ccgacaaccg gaagggtccc ggcgagatcc ggctccgcgc tccaggcgct
     3961 gcgcgtcgcc gcgtcgagct ggtcctgggc gcgcgcgagg ctcccgccgg gacgcaggcg
     4021 cccgactacc cagaaccccc attcgtgggc tcgcctcgcc cccgagggcc ctgacagggc
     4081 ggaaccgaga ttgctccaga cgtcggcatc cgccggatag cggaaacggc tcggcatgac
     4141 cccgacgatc gtatagacaa cggtgtcgag cgtgatcgcc cggccgagca cggcgcggtc
     4201 gccgccgaag cgcgtggtcc agaatgcctg gctcaggatc gccacgggag cgctgcccgg
     4261 gcggtcctcg tccacggcga acccccttcc gagcagcggc cgagccccga gcaacggaag
     4321 gaagttggcg gagacgtgcc acgccacgac gcgctcggcg gggcccatgc cggtcagcac
     4381 cgcgtcgtcg ccggtgtagg cggccatgtc ctcgaaggcg gcggtggccg tccgccaggt
     4441 ccggtaagaa gccaggggcg acgtggcgcc accgatcatc cccgcgtggc gggactccgc
     4501 gcgcaccagc ttgatgtcca ccagccgctc ggcgtgcggg aagggcaggg accggagcaa
     4561 caggccctgc accacgctga acacggcggt cgtcgccccg atgcccagcc ccaaggtgac
     4621 gatggcgacg agcgcgaacc ccggcgcctt ggcgagggtg cggagggcgt agcggagatc
     4681 ttgcagcaga gcgtccattg tgtctccatg caggtgccag gttccaggtg tctggtgtca
     4741 gggtgaacca cctgacatct ggcccctggc acccggcacc tattcagttc tcaacgccac
     4801 catcggatcc agccgcgctg cacggcgcgc cgcgacgtcg ctcgccagga gcgccacgag
     4861 gcccagggtg agcgccacgc ccaccagggt gaggggatcg cgtgcgccga cgccgtagag
     4921 catggtggtg agcacccggg tcaacgccag cgcccccgcc aggccgagga ccagccccgc
     4981 gccggcgagc atcgcgccct gtcccacgac cagatcccgc acccgtcggg gctcggcccc
     5041 cagcgcgatg cggattccga tctcccgcgt ccgttgggtg acggtgtagc tgagcaggcc
     5101 gtatatgccg atcacggcga gcaacaacgc cacggccgcg aacagcacca ggacgatcat
     5161 cgtgaagcgc tgctgggcga cggtgttcga caacgtcgcc gccagcggct ccactccgaa
     5221 aagcggcagc tcgggatcga ggtccgccac gacgcgacgc aacgccggag tgaacgcacc
     5281 caggtcgccg tgcacgcgca ccaggacggt gtggccgcca tccgccactg ggacctgagc
     5341 gagcggcagg taaagccccg gcggagtcgc ggccatgaga ccgcggaacc gctcgtttcc
     5401 caccaccccc accacgagcc gctcgccgcc ccacagccgg atgcgttggc caagcgggtc
     5461 ctgcgtcggg aagaagcgcc ggcgggcggc ttcgttgatg accacgacgg tcggcgcgtc
     5521 cacggcgtcc gttgcctcga ggtaccgccc cgccagcagc gggacgccga gggtcccgaa
     5581 atacgtggag gtcacgatcc ggatcgccgg ctccggccag tcgccggcct ccgcctcccg
     5641 tcccacgacc gcgatggagc tcgtgaatcc cgcgttcgcc gggtgattcg cggcgatggc
     5701 gactgcggcc actcccggga gc
//
LOCUS       DBNGZW010000554         2427 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_663756, whole genome shotgun sequence.
ACCESSION   DBNGZW010000554 DBNGZW010000000
VERSION     DBNGZW010000554.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_663756"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..469)
                     /locus_tag="AC16JC_13180"
     CDS             complement(<1..469)
                     /locus_tag="AC16JC_13180"
                     /inference="COORDINATES: protein motif:HMM:NF045460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR5165696.1"
                     /translation="MKLPDAWRRGCVAVIGLGKSGTAATRLLVREGVRVYASDASPAP
                     YAGAAAETLRSLPGVEVELGWHDPAKIRDAAAVVVSPGVPPDAPPLVAARAAGVPIIS
                     ELDLGFRSLSGVRCIAVTGTNGKTTTTALIAHLLAQAGLRAEAAGNIGRPLADV"
     gene            complement(466..1584)
                     /gene="mraY"
                     /locus_tag="AC16JC_13185"
     CDS             complement(466..1584)
                     /gene="mraY"
                     /locus_tag="AC16JC_13185"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008618878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR5165697.1"
                     /translation="MLYHLLTPLARQHIIFNLFNYITFRTAGATVTALILAFAVGPWV
                     IRRLRERGVGQVIRADGPAHQGKRGTPTMGGVIIILATVVPTLLWARLDTRYVLVAVA
                     ATLWMGAIGFVDDYLKIVRGKSQGLVARWKLAGQVSFGLALGIYLVLAPLAPQLGAAT
                     TQMPFFKYILLGLPWWLYLLFVTVVVTGSSNAVNLTDGLDGLATGLTGIVAGCFAIFA
                     YLFGRFDAAHWLLVYYLPDSGELAIFCGALMGASLGFLWFNAHPASVFMGDTGALAIG
                     GALGTVAIMLKAEFLLLIAGGVFVAEALSVLIQVSVYRLYKRTRGKEYADAHRVFRMA
                     PLHHHFEKLGWAEPQVVMRFYILGFLCAMVALATLKVR"
     gene            complement(1601..>2427)
                     /locus_tag="AC16JC_13190"
     CDS             complement(1601..>2427)
                     /locus_tag="AC16JC_13190"
                     /inference="COORDINATES: protein motif:HMM:NF014874.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate ligase domain-containing protein"
                     /protein_id="HIR5165698.1"
                     /translation="LRAVIEPTIAVITTVGFAPVEGFGSLAGVLHEKLSLLDGVPLAV
                     VGTDPPELGREARRRTRTVVAGSTPAAEFRVDSVRLDEDGRCVLRRLGREVTLPLFGL
                     HQGDNAMLALAVAQEAGVEAGAALAALSGVAVPGGRGRVSRRGDLTIIDDTYNANPGS
                     LARAVESAVQLARARGRPLAVVVGSMLELGPESARLHAAAAADIVRCEPELVGAVGEF
                     AAAFAPYRGRLGERLLVAENAAALGPRLRAALRGNEVVLLKASRGVALEQVLPHLS"
BASE COUNT          405 a          926 c          804 g          292 t
ORIGIN      
        1 ccacatccgc cagcggtcgt ccgatgttgc cggccgcctc ggcccggaga ccggcctggg
       61 cgagcagatg ggcgatgagc gccgtcgtcg tcgtcttccc gttcgtcccc gtgacggcga
      121 tgcagcgcac gccggacagg ctgcgaaacc cgagatcgag ctcggaaatg atcggcacgc
      181 ccgccgcgcg cgccgcgacg agcggcggcg catccggcgg caccccgggc gacaccacga
      241 cggccgcggc gtcccggatc ttcgccggat cgtgccaccc gagctcgacc tcgacccccg
      301 gtagcgagcg cagcgtttcc gccgccgcgc ccgcgtaggg cgccggactg gcatcgctgg
      361 cgtagacccg cacgccttcc cgcaccagca gccgcgtggc cgccgtgccg ctcttgccga
      421 ggccgatcac ggccacgcac cccctgcgcc aggcgtccgg cagcttcatc gcaccttgag
      481 cgtggcgagc gccaccatgg cgcagagaaa cccgaggatg tagaaccgca tcaccacctg
      541 cggctccgcc cagccgagct tttcgaagtg gtggtgcaac ggcgccatgc ggaacacccg
      601 gtgcgcatcc gcatactcct tgccgcgcgt ccgcttgtac agccggtaca ccgacacctg
      661 gatcaggacc gaaagcgcct cggcgacgaa caccccgccc gcgatgagca gcaggaattc
      721 cgccttgagc atgatggcca ccgtgccgag ggcaccgccg atcgccaacg ctccggtgtc
      781 gcccatgaag acggacgcgg ggtgggcgtt gaaccacagg aacccgaggg acgcgcccat
      841 cagcgcgccg cagaagatcg cgagttcccc ggagtcgggg aggtagtaca ccagcagcca
      901 gtgcgccgcg tcgaagcgcc cgaagagata ggcgaagatc gcgaagcatc cggccacgat
      961 cccggtgagg ccggtcgcca ggccgtcgag cccgtcggtg aggttgaccg cgttggagct
     1021 gccggtgacc acgaccgtca cgaacagcag gtacagccac cacggcagcc cgagcaggat
     1081 gtacttgaag aacggcatct gcgtcgtcgc cgctccgagc tgcggggcga gcggcgccag
     1141 aaccaggtag atgccgagtg cgagcccgaa gctcacctgc ccggccaact tccaccgcgc
     1201 cacgagtccc tgcgacttgc cgcggacgat cttcagataa tcgtccacga atccgatggc
     1261 gcccatccac agcgtcgccg ccacggcgac cagcacgtac cgcgtgtcga gccgggccca
     1321 caggagcgtg ggaaccaccg tggccagaat gatgatgacg ccgcccatgg tgggcgtccc
     1381 gcgcttcccc tggtgcgccg gcccgtcggc gcggatgacc tgccccacac cgcgctcgcg
     1441 cagccgccgg atcacccagg gacccaccgc gaacgccagg atcagcgcgg tgaccgtcgc
     1501 gccggcggtc cggaaggtga tgtagttgaa cagattgaag atgatgtgct gcctggcgag
     1561 cggcgtcagc aggtgataga gcacggtggc cggggtctcc ctagctgaga tggggcagca
     1621 cctgctccag ggcgacgccg cgcgacgcct tgagcagtac gacctcattc ccccgcagcg
     1681 cggcccggag gcgcgggccc agcgccgcgg cattctccgc gacgagcaac cgctctccaa
     1741 gccggccgcg gtacggcgcg aacgcggcgg cgaactcccc gaccgcaccg accaattcgg
     1801 gctcgcaacg cacgatgtcg gccgccgccg ccgcgtgcaa tctcgcactc tcgggcccaa
     1861 gctccaacat cgagcccacc acgaccgcca gcgggcgtcc ccgcgcgcgc gccagctgca
     1921 ccgccgactc caccgcccgt gcgagcgagc ccgggttcgc gttgtaggtg tcgtcgatga
     1981 tcgtgagatc gccccggcgc gatacccgtc cccgcccgcc cggaaccgcg acgccggaca
     2041 gggccgccag tgccgccccc gcttccaccc ccgcttcctg cgccaccgcc agcgcgagca
     2101 tcgcgttgtc gccttggtgc agtccaaaca aaggaagggt gacctcccgg ccgagccggc
     2161 ggaggacgca ccgcccgtcc tcgtccagcc gcaccgagtc gacccggaac tccgccgcgg
     2221 gggtcgagcc cgccacgacg gtgcgggtgc ggcgccgcgc ctcgcgcccg agctccggcg
     2281 gatcggtgcc caccaccgcc aggggcacgc cgtcgagcag cgacaacttc tcgtgcagca
     2341 cgccggccag cgagccgaag ccctcgacgg gggcgaagcc cacggtggtg atcaccgcga
     2401 tcgtcggctc gatgacggcg cgcagcc
//
LOCUS       DBNGZW010000555         4390 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1686257, whole genome shotgun sequence.
ACCESSION   DBNGZW010000555 DBNGZW010000000
VERSION     DBNGZW010000555.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4390)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1686257"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1106
                     /locus_tag="AC16JC_13195"
     CDS             <1..1106
                     /locus_tag="AC16JC_13195"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5165699.1"
                     /translation="ADDLTLEDWLAKRIWPLEAAHTPDTVYWSAMLGAAELLLGGTTA
                     ILDMETVRHTAAAFNALEEIGLRATAGKCLMDAETNSAGLFERTGRALQEAADLCARW
                     HGAANGRLRYCFAPRFAPSCTSSLLRAVSDAAERAGAVIHTHAAETPVELELVKRATG
                     QDEITYLDSVGIAGPRAALAHCVWVDRESMGQLARAGTNVVHCPSSNLKLGSGIAPIP
                     ELLATGCHVALGADGAPCNNRLDGFEEMRLAALIQKPRLGPEALPAAKVLELATMGGA
                     RALGLEHEVGSIEVGKRADLVAIDLSGPHGQPADADLASRLVYAARAADVRHVVVDGR
                     IVVRSGRLLTADLEEIRVKANAAARRLRRAVGL"
     gene            1054..2226
                     /locus_tag="AC16JC_13200"
     CDS             1054..2226
                     /locus_tag="AC16JC_13200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HIR5165700.1"
                     /translation="MSKRTPLLAVCAALWVCSAAAQEPARVIPIYLVPRDLSFPMSGV
                     VYHARVMEDVRRWYGAVLGGRTFVAEPLVVQVSRYTFAELAADSFQAWWPLLQREFAD
                     YGWSWNRRAKFKLLFLTHGAGAWAGSDSENGGIDSTADAGRVDKGNWGGLAVIGDSSV
                     GGVAAGVCPEVAHGKADSTVATAWWCSGSTYRGTVAHELGHTFGLPHPDAFRKGFRCA
                     DSTAYTIMQCHWGWGRAGLLDYEIAHLRSLDFFQFDTAPAYTLLPAQRPLSAAGSRVT
                     RLERGDSLVWLDGRGGGTGYPWAIALTRTGAALRYATAGGRVVAFDLGLARGSVGEAA
                     LAVLMDGRAVGLYQVIAGHPPQRIVLEAHSAREVTFRLARRRGTGAVVLGNPRLYR"
     gene            2420..>4390
                     /locus_tag="AC16JC_13205"
     CDS             2420..>4390
                     /locus_tag="AC16JC_13205"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HIR5165701.1"
                     /translation="MNTLTADFRFAFRQLLRRPGFTVVAVLTLALGSGAAAAMFGIVD
                     RVLLRPLPFPEADRLVALCETNPSVEGFCVASPPNVEDWSRRTRALTSVGLGRTWPFS
                     MRAESGSQGVGGGLATPGFFRTLRVTPLLGRTFTPEDLTPSGRHVAVLSAGFWQSRFG
                     GSRDVLGRAIVLDAESYEVVGVLGADADIPRFSEVQVWVPLPFDPRDEENRRWRGFET
                     IGRLAPGVSVAAAQRELQAIQQDLALSHPATNRDWSARIVPLLDRVVGPVRPTLLVFM
                     GAVVILLVVAGANVTNLLVARGAARERELAVRAAIGASRGALFRLVAAEGFVLAAVGG
                     VVGLLVAAWAADGLLRLMPNGLPRVTHVGLDGRVLGFTLVLTVAVGTLAPLLPALRAM
                     RLDLTIALKQGQPTGWRSALGLRGGLVVFEVATAVLLAIGAGLLARSFTTLLRWQPGF
                     ERAHLLTFWTLASPGTYKDAPSVATLFARVEGELRSLPGVSGVGMVSSGALFGGEETG
                     EFALGGAGGGGEPYLAARWYDMSATYFPTLGLPLRRGRFFGGADRAGAPPVALINETM
                     ARRLFGVADPVGRRLRERNDDRAIEIVGVVADIPPFTPGVPSQPEIYWPYAQRPRWAS
                     YFVLRTSGDPATLAQAVDSRLASVDPDLRPGRV"
BASE COUNT          561 a         1479 c         1611 g          739 t
ORIGIN      
        1 tcgccgacga cctcacgctg gaagactggc tggcgaagcg catctggccg ctcgaggccg
       61 cccacacgcc ggatacggtc tactggtcgg ccatgctcgg cgccgccgag ctgctgcttg
      121 gcggcaccac ggcgatcctc gacatggaga cggtgcgcca caccgctgct gcctttaacg
      181 cgctcgagga gatcggcctg cgcgccacgg cgggcaagtg cctcatggat gcggagacga
      241 attccgccgg actgtttgag cgcaccggcc gcgcgctcca ggaggcggcc gatctatgcg
      301 cgcgctggca cggcgcggcg aatgggcgcc tgcgttactg cttcgccccc cgcttcgcgc
      361 cgagctgcac cagctcgttg ctgcgggcgg tgagcgatgc ggcggagcgc gccggcgccg
      421 tcatccacac gcatgcggct gagacgccgg tcgagctgga gctggtcaag cgcgccaccg
      481 gccaggacga gatcacctat ctcgattccg tggggatcgc gggaccccgc gcggcgctcg
      541 cccactgtgt ctgggtggac cgggaatcga tgggacagct ggcccgggcc gggacgaacg
      601 tcgtgcactg tcccagctcc aacctgaagc tcggcagcgg catcgccccg atcccggagc
      661 tgctcgccac cggctgtcac gtggcgctgg gcgccgacgg ggcgccgtgc aacaaccggc
      721 tcgacgggtt cgaggagatg cgcctcgccg cgttgatcca gaagccgaga ctgggacccg
      781 aagcgttgcc ggcggcgaag gtgctcgagc tcgccaccat gggcggggct cgggccctgg
      841 ggctcgagca cgaggtcggc tcgatcgaag tcgggaagcg cgccgatctc gtggcgatcg
      901 acctatccgg cccgcacggc cagccggccg acgccgatct cgcctctcgc ctcgtctacg
      961 ccgcccgcgc cgccgacgta cgtcatgtcg tggtggatgg ccgcatcgtg gtgcggagtg
     1021 gccgcctgct caccgccgac ctcgaggaga tccgtgtcaa agcgaacgcc gctgctcgcc
     1081 gtttgcgccg cgctgtgggt ctgtagcgcc gcggcgcagg agcccgcgcg cgtgatccct
     1141 atctatctgg tgccgcgcga tctgtcgttc cccatgagcg gcgtcgtcta tcatgcccgc
     1201 gtgatggaag acgtgcgccg ctggtacggc gcggtgctgg gcggccgcac cttcgtcgcc
     1261 gagccactcg tcgtgcaggt gagccggtac accttcgccg agctggcggc cgacagcttc
     1321 caggcgtggt ggccgctgct gcagcgggag ttcgccgact acggctggtc gtggaatcgg
     1381 cgggcgaagt tcaagctgct gttcctgacg cacggcgccg gggcgtgggc gggctccgat
     1441 tccgagaacg gcgggatcga cagcacggcg gatgcgggcc gggtggacaa ggggaactgg
     1501 ggcgggctcg cggtgatcgg cgactcgagc gtaggtgggg tcgcagcggg cgtgtgtcca
     1561 gaggtcgcgc acggcaaggc ggattccacg gtcgccaccg cctggtggtg ctcggggtcc
     1621 acctaccgcg gcacggtcgc gcacgagctg gggcatacct tcggcctgcc gcaccccgac
     1681 gcgttccgga aaggcttccg ctgcgccgac tcgaccgcgt acaccatcat gcagtgccac
     1741 tggggatggg gccgggccgg cctgctcgac tacgagatcg cccacctgcg cagtctggac
     1801 ttctttcaat tcgataccgc cccggcctac accctgctcc cggcgcaacg accgctgtcg
     1861 gcggccggtt cgcgcgtgac ccgcctggag cggggagatt cgctcgtgtg gctcgacgga
     1921 cgcgggggcg gtacgggata cccatgggcg atcgccttga cgcgcaccgg agccgcactg
     1981 cgctacgcga ccgccggggg acgcgtcgtt gcattcgatc tcggactggc ccgggggtcg
     2041 gtgggcgagg cggcccttgc cgtgctgatg gatgggcggg cggtcggctt gtaccaggtg
     2101 atcgccgggc atcctccgca acgcatcgtg ctcgaggcgc actcggcgcg ggaggtgacg
     2161 ttccgactcg cgcgaaggcg ggggactggc gccgtggtgt tgggaaatcc ccgactgtat
     2221 cgctgacgcc ggccttcaca ctcttctcat gctcctggcg tggagggtgg tgtccggtaa
     2281 tggaactcct cttcccagaa gcgcgcgctc caatgactct cgcgcgagag caattcactc
     2341 acgcgcaggg acttggacac tgccggcagg cggtccgtaa gttgcccctc cttggcgtgc
     2401 gcctctcgac gaggacaccg tgaacacgct gactgctgac ttccgtttcg catttcgcca
     2461 gctgctgcgc cgccccggct tcaccgtcgt cgccgtgctg accctcgcgc tcggcagcgg
     2521 ggcggccgcg gcaatgttcg gcatcgtcga tcgtgtgctg ctgcgaccgc tgccgttccc
     2581 cgaggcggat cgcctggtgg cactgtgcga gacgaatccg tccgtcgagg gcttctgtgt
     2641 ggcctcgccg cccaatgtcg aggactggtc gcgacgcacc cgtgcgctga cgtcggtggg
     2701 gctcggccgg acctggccct tttcgatgcg tgccgaatcc gggagccagg gggtcggcgg
     2761 cggcctagcg accccgggct tcttccggac gctgcgcgtg accccgctgc tcgggcggac
     2821 gttcacgccg gaggacctga cgccatccgg tcggcatgtc gcagtgctga gtgccggatt
     2881 ctggcagagc cgcttcggcg ggagtcgcga cgtgctcggc cgcgcgatcg tgctcgatgc
     2941 ggaaagctac gaggtggtcg gcgttctcgg agcggatgcc gacatcccac gcttttccga
     3001 ggtacaggtc tgggttccat tgccgttcga tccccgggat gaggagaacc ggcgttggcg
     3061 cggcttcgaa accatcggcc ggttggctcc gggcgtcagc gtcgcggcgg cgcagcgtga
     3121 gctgcaggcg attcagcagg atctcgccct gagccacccc gcaacgaacc gcgattggag
     3181 tgcgcgcatc gttccgctgc tggacagggt ggtcgggccc gtccgtccca cgctgctcgt
     3241 gttcatgggt gccgtcgtga ttctcctggt ggtcgccggc gccaacgtga ccaacctgct
     3301 cgtggcccgg ggagccgcgc gcgagcggga gctggcggtg cgcgcggcga tcggggcgag
     3361 ccgcggagcg ctattccggc tggtggctgc ggagggcttc gtgctcgccg ccgtcggcgg
     3421 agtggtcggc ttgctcgtcg ccgcgtgggc cgccgacggg ctgttgcggt tgatgccgaa
     3481 cggcctgccc cgggtgacgc acgtcggctt ggatgggcgc gtgctcggat tcaccctggt
     3541 gctcaccgtc gccgtcggga cgcttgcgcc gctcctaccg gcgctgcgtg cgatgcgtct
     3601 ggacctcacc attgccctca aacaggggca gccgacgggg tggcgctcgg cgctcgggtt
     3661 gcgagggggg ctggtggtgt tcgaagtggc caccgccgtt ctgctggcga tcggcgcggg
     3721 cctgctggcg cgcagtttca ccacgctgct ccggtggcag cccggtttcg aacgcgccca
     3781 cctgctcacg ttctggaccc tcgcctcgcc aggcacgtac aaggacgctc cgagcgtcgc
     3841 gacccttttc gcgcgcgtgg aaggtgagct gcgcagcctc cccggcgtgt ccggagtggg
     3901 gatggtgtcc agtggggcgc tgttcggagg tgaggaaacg ggcgagttcg cgttaggtgg
     3961 cgctggagga ggcggagagc cttatctggc agcccgctgg tacgacatga gcgcgaccta
     4021 ctttccgacg ctcggcctgc cgctgcgccg cggccggttc ttcggtgggg ccgaccgcgc
     4081 gggggctccg cccgtcgcgt tgatcaacga gaccatggcg cggcggctct tcggggtcgc
     4141 cgaccccgtg gggcggcgcc tgcgcgagcg gaacgacgat cgcgcgatcg agatcgtggg
     4201 ggtcgtggcg gacattcctc cgttcacgcc cggcgtcccg tcccaaccgg agatctactg
     4261 gccgtatgcg cagcggcccc ggtgggccag ctacttcgtt ctgcgaacga gcggcgatcc
     4321 cgcgactttg gcccaggcgg tggacagcag gctggccagt gtcgaccccg acctgcggcc
     4381 cggccgcgtc
//
LOCUS       DBNGZW010000556         2274 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1144353, whole genome shotgun sequence.
ACCESSION   DBNGZW010000556 DBNGZW010000000
VERSION     DBNGZW010000556.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1144353"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..565)
                     /locus_tag="AC16JC_13210"
     CDS             complement(<1..565)
                     /locus_tag="AC16JC_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR5165702.1"
                     /translation="MKAVRSDTFGGPARLVDVPDPTPTPDGAVVRVGACGLCRSDWHG
                     WKGRDADVKVPHVSGHEFAGTVIEVGTDVRKWKQGDRITVPFSMGCGTCERCRTGDEH
                     ICDRYYQPGFTGWGTFAEYVALPYADGNLVRLPDAMTLTTAALLGCRFATAYRAVVGQ
                     GRTRPGEWVAVHGCCRAAPRRSERAASA"
     gene            604..1896
                     /locus_tag="AC16JC_13215"
     CDS             604..1896
                     /locus_tag="AC16JC_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011755786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5165703.1"
                     /translation="MTAARLAPAEVDVRRIQRRTVAVLMVSQALGGVGVSSGVAVAAL
                     LAEDILGSAGLAGLAQTSQVLGAALAAFLLARVMAARGRRAGLVLGYGIGGIGAALCV
                     LAGVVHSFPVLLLGTLAIGSATAANNQARYAATDLARPDHRARALALVVWATTLGSVL
                     GPNLAGPGAAVARALGTPALTGAFVFTALGTWLATTWIAVRLRPDPLLLAWEQAHSRG
                     ESPRSHATLRHIIGVLRMHPRAAAALVAMASAHAIMVSVMVMTPLHIHHGDPAAAGAT
                     LRIIGLVLSAHVLGMYALAPLVGWLVDRIGHAAVLATGGFVLLAALTLAGGAPAGGSP
                     RLFVGLFLLGVGWSCSLVASSALLVDAVPLLERPGVQGGADLLMGLFAAGAGALGGVI
                     VGQWGYAVLNVAAGVLALSVLGAAVVGRDRSPIPARPS"
BASE COUNT          277 a          787 c          826 g          384 t
ORIGIN      
        1 gggcactggc ggctcgctct gatcggcgag gagcagcccg acagcagccg tgcacggcca
       61 cccattcacc gggccgcgtg cggccctgcc cgacaacggc gcggtacgcc gtcgcgaagc
      121 ggcaccccag cagcgcggcg gtcgtaaggg tcatggcatc ggggaggcgc accaggttcc
      181 cgtcggcgta cgggagggcg acgtactccg cgaacgtgcc ccagccggtg aagcccggct
      241 ggtagtagcg gtcgcagatg tgctcgtcgc ccgtacggca gcgctcgcac gtgccgcatc
      301 ccatcgagaa ggggacggtg atccggtcgc cctgcttcca tttgcggacg tctgtgccca
      361 cctcgatcac ggttccggcg aactcgtgcc ccgacacgtg cggcactttc acgtcggcgt
      421 cgcggccttt ccatccgtgc caatcggatc ggcagaggcc gcaggctccc actcgtacga
      481 cggcgccatc gggagtcggc gtgggatcgg ggacgtcgac cagacgagcc ggacctccga
      541 atgtgtcgga gcgaactgct ttcatccgag aaatatgtcc ctggtgcgag gtactttccg
      601 gccatgacgg ccgcccgctt ggcgcctgcc gaggtcgacg tccgtcgaat ccaacggcgg
      661 acggtggcgg tgttgatggt cagccaggcg ctcggcggcg tcggcgtctc gtcgggcgtc
      721 gccgtcgccg ccctgctcgc ggaggacatc ctcggctcgg ccggtctcgc cggtctggcg
      781 cagacgagtc aggtgctcgg cgcggcatta gccgcgtttc tgctcgcccg cgtaatggcg
      841 gcccgtggcc gccgcgccgg tctcgtcctc ggctatggca tcggcggaat cggtgccgcg
      901 ctgtgcgtcc tcgcaggcgt cgtgcactcg ttcccggtgc tgctgctcgg caccctggcg
      961 atcgggtcgg cgaccgccgc caacaaccag gcgcggtacg ccgcgaccga cctcgcccgg
     1021 ccggaccacc gcgcccgcgc cctggcgctc gtagtatggg cgacgacgct cggctccgtg
     1081 ctggggccga acctcgctgg tccgggcgcg gcggtggcgc gtgcgctcgg cacgcccgcg
     1141 ctgaccggcg cgttcgtctt caccgcgctc gggacgtggc tcgcgacgac gtggatcgcc
     1201 gtgcggttac ggcccgaccc cttgctcctc gcctgggagc aggcgcactc gcgcggcgag
     1261 tcccctcgct cccatgcgac gctgcggcac atcatcggcg tgctgcgcat gcacccgcgg
     1321 gccgcggccg cgctggtcgc catggcgagc gcccacgcga tcatggtgtc ggtgatggtg
     1381 atgacaccgc tccacatcca ccacggtgac cccgcagcag ccggcgcgac cctgagaatc
     1441 atcggcctgg tgctgagcgc gcatgtgttg ggcatgtacg cgctcgcgcc gctcgtgggc
     1501 tggctcgtgg accggatcgg ccatgccgcc gtgctggcga ccggagggtt cgtgttgctg
     1561 gcggccctca cgctggctgg gggggcgccc gccggtggtt cgcccaggct cttcgtgggt
     1621 ctcttcctgc tgggcgtggg ctggtcgtgc tctctggtcg cgagctccgc cctgctcgtg
     1681 gacgccgtgc cgctgctcga gcgaccgggg gtgcaaggcg gcgcagatct attgatggga
     1741 ctgttcgcgg cgggtgcggg ggcgttgggg ggcgtgatcg tggggcaatg gggatacgcg
     1801 gtgctcaacg tggccgccgg ggtcctggcg ttgtcggtct tgggcgccgc ggtcgtcggg
     1861 cgagatcgtt ctccgattcc tgcgcgcccg agctagtacg cgccacgatc gaggccgtcg
     1921 gtatcatgtc agctacggcg gcggtgctcc atccacgagc gtccgccccg gctggatccg
     1981 gtcgacgcgc cacatcatct atccctgcgg cgtccgaatc cacgactcgc gctgcaccac
     2041 cgacctccgg atttcgtgga gcgtgctgtc cgggaagcgt tggtggcgca cggtgcgctg
     2101 cgcgaacgaa gtcctcgtag ggcatcgact tcacacctgt gtgcgcggcc tagcgtggag
     2161 gcacacgacg tggacctgga ggggcccgga cccgtccccg acgcgcgcga cgtgaagcgc
     2221 tagtgcgcgg tgtctcatcg cggcatcttg accgtgcagg gcgatcgagg agaa
//
LOCUS       DBNGZW010000557         7385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1811150, whole genome shotgun sequence.
ACCESSION   DBNGZW010000557 DBNGZW010000000
VERSION     DBNGZW010000557.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7385
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1811150"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="AC16JC_13220"
     CDS             complement(<1..747)
                     /locus_tag="AC16JC_13220"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5165704.1"
                     /translation="MRAFVTGATGFVGAHLVDALLRRGDTVVCLVRTAAKARALGWTG
                     VRLLEGDLDTPDVLREGCAGAEVVYHVAGRISARNPGEFLAVNRDGTARVLDAAGREP
                     PRRLVYVSSLAVAGPTVPGQPIDEGRAPAPITAYGRSKLAAEELVRASAVPWTIVRPP
                     VVYGERDRETLRIFRLARIGLGTVIGDGSQELSVVYAPDLAAALIAAGLAPAAAGRVY
                     YAAHPAATTSWGLVEAVGRAVGTRPRRLPIP"
     gene            complement(744..1856)
                     /locus_tag="AC16JC_13225"
     CDS             complement(744..1856)
                     /locus_tag="AC16JC_13225"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020383377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5165705.1"
                     /translation="MSVEVAPVRSGRDLERFIAFPYTLHRRDPMWVPPLRMDVRTILS
                     PRKNPFFQHAQAEYFIARDAGRIVGRIAAIRNEAHNRLHHDRVGFFGFFECVDDPAPA
                     IALFDAAARWLHEHGLDTMRGPMNPSINDECGLLVEGFDTPPVVLMPHNPAYYVPLHE
                     RYGFHKAKDLIAFWGGGEAPPERFERANRVIAERLGVTLRSLDMKRFRQEVDLVKALF
                     NEAWEQNWGYVPMTDAELEHLAKQLKPIIIPELVVFAEIQGRVVGFGVAIPDFNVALK
                     HNPSGRLFPGLLKILWYSRRIDRARIPLLGTLRQYRGKGIDAMLYHWIWTKSAARGMA
                     KGEAGWILEDNAPMINGLIRLGFHPYKTYRVYDKAL"
     gene            complement(1853..3034)
                     /locus_tag="AC16JC_13230"
     CDS             complement(1853..3034)
                     /locus_tag="AC16JC_13230"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018198478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5165706.1"
                     /translation="MALFDKCRGFTKAREIQAAGLYPYFKPISRSEDTVVVIEGKERI
                     MMGSNNYLGLTHHPEVLAAARAALERYGSGCTGSRFLNGTLDLHEQLEAELAEFFGKE
                     SCLVFSTGYQANLGVISGLVGRGDVVFLDKLDHASIVDGAKMSQGETVRFNHGDLAGL
                     ERRLQKVPEGTGTMVIVDGVYSMEGDIADLPNLIKLAQRYGVALAVDDAHSVGVLGPK
                     GDGTAAHFGLTEEIDLIVGTFSKSLASIGGFAAGDETVIHYLKHHSRPLIFTAALPPA
                     NTAGVLAALHVLQRDPERRDRLWINTRRLQQGLRGLGFDIGPTETPIVPVLIGPLEKT
                     FLFWRKLFDAGVFTNPVVPPAVPPSQCRLRTSLMATHTFEQIDRAIETFARLGKELEV
                     I"
     gene            3177..3908
                     /locus_tag="AC16JC_13235"
     CDS             3177..3908
                     /locus_tag="AC16JC_13235"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR5165707.1"
                     /translation="MFHSCTGARRLTVLCAGLALGGGSLAAQHAPPRYTAGWGDVIAV
                     SGAGLLTAAGALAHPPAAACAPCNPADLTGFDRGVIDWNSTAARRGSDILLLSVVGGA
                     ALASVGGMEPARARGDAAVLANALLWTTAATEWLKVGIHRARPALYRSGAVEAAAVHE
                     NRESFPSGHAAVAFAAATAYATLAARQHLPHATRNAILLYAGAASVSVLRVAGGKHFP
                     TDVLGGAALGAGIGWTVARLHPMTR"
     gene            3905..5311
                     /locus_tag="AC16JC_13240"
     CDS             3905..5311
                     /locus_tag="AC16JC_13240"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5165708.1"
                     /translation="MSDPGHGSGQPPRVMGLRDVVLFNITAIVGLRWLTTASSQFGLA
                     ALALWLVAMIVFFIPSGIAVRELADIDPGQGGLYRWVARAFGNRHAFVAGWGYWVNNL
                     FYFPSLLVTTAAIAAYAGGPRTAPLGEDSTFILVVSLIGLWIAVWLNLVGLRVGKWLQ
                     NLGGWGTWLPALLFVGLAAWSLVQRGSATPFTARGLLPARLDLPSINLFATMTFAFAG
                     LELAPSLGGEIVDPAATLRRGVLLSGIAIVALYGLGTVAMLVALPHETVSVTNGMPQA
                     VQALADRLAIPGLRIAAPLTAVLLTFGNLGGVGAWLAGSARLPFAAGVDRALPPAFAR
                     LHPRWRTPYVSLLLMAVLASAFIVASLAGNTVKSAYLLLTQTTLILFFIPYLYIFAAY
                     LRLRRRRTAVTLLTGLLGLAAVAFSIVLGFVVPGDEAHPDAYRLKVVVGVVVFMGIGF
                     WLSRRSVRGPTATAASAP"
     gene            5337..>7385
                     /locus_tag="AC16JC_13245"
     CDS             5337..>7385
                     /locus_tag="AC16JC_13245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HIR5165709.1"
                     /translation="MPLVRSYFLVCAASCLASVAGAQGRPAAARQRPGPRWEIPGLDI
                     TPNGGWRVRARAVAATRARLLAQRNFALLNAAPVGPTAVNAVTGALRVPVVMFRYQDS
                     PAGQYSRDTTQYNATLFGTVPPAGRPYTLRSFYEQLSNGLFSIQGAAAGWEALAGNES
                     AYTGVAGTCPGNPYGTNNCNGIWSTAAFTAMLSGLQEALTHADSTIDFGQYDNDGPDG
                     IPNSDDDDGVVDAVLFLHPSLDGACLSATNNHLWSHRFSLNDFATNDAATPGPKKTSS
                     HVLVRDYILQSGLGSSLPSGSPCGTDAIMPIGTAAHELGHILALPDLYDVSGSTEGIG
                     EFGLMGSGNYTTGNSPSRYDAWSLQQMGWTTIQPLTTTGTYTVGPVPTADTVFYVNVQ
                     GANPDGEYFLIENRQEVLSDSAMLRVHCALSGDAPGCAGGFLIYHVDGLKACMINVCG
                     NSVNAGSIHGVAIEEADGLRNLWNGDNRGDGGDLYPGTTDNTALAFRTSPAATKNTDT
                     SFAGFAVDSIRQLVPNGAMAFRLRFGSLTSVAANDTAAEVQVDGQPYNVFQDLFDEGS
                     SHTIAVADSQLSTSGRTRLTFQSWSDGGAISHVITGAAAGATYTATLAREHRLDVTVG
                     TNGAVAYSPVADSSGSFILQGTPVTLTATPTAPFVFGGWTGDTTAAGALLVLPMGRPF
                     A"
BASE COUNT         1075 a         2519 c         2602 g         1189 t
ORIGIN      
        1 gggaatcggc agcctccgcg gccgcgttcc caccgcccgc cccaccgcct cgaccagccc
       61 ccagctggtc gtcgcggcgg ggtgcgccgc atagtagacc cgcccggccg ccgccggggc
      121 gaggcccgcg gcgatgagcg cggccgccag gtccggcgca tacacgaccg acaattcctg
      181 cgatccgtca ccgatcaccg tccccaggcc gatgcgcgcc aggcgaaaga tgcgcagcgt
      241 ttcccggtcc cgctccccgt agaccacggg cggccggacg atggtccacg gcacggcgga
      301 ggcgcgcacc agctcctccg ccgcgagctt gctccggccg taggcggtga tcggcgcggg
      361 ggctcggcct tcgtcgatgg gctgcccggg caccgtcggt cccgccacgg ccagggagga
      421 cacgtacacg aggcggcggg gcggctcgcg gcccgcggcg tccagcaccc gcgccgtgcc
      481 gtctctgttg acggccagga actcccccgg gtttcgtgcg ctgatgcgtc cggcgacatg
      541 gtagacgacc tcggctccgg cgcagccttc ccgcagcacg tccggggtgt cgaggtcacc
      601 ctcgagcagg cggacgccgg tccatcccag cgcccgcgct ttggcggcgg tgcggaccag
      661 gcacaccacc gtgtcgcccc gtcgaagcag ggcgtcgacg agatgggcgc ccacgaagcc
      721 ggtggcgccc gtgacgaacg ctctcacaac gccttgtcgt agacgcgata cgtcttgtag
      781 ggatggaaac cgagccggat gagtccgttg atcatcggcg cgttgtcctc gaggatccag
      841 cccgcttccc ccttggccat gccacgcgcc gcggacttgg tccagatcca gtgatacagc
      901 atggcgtcga tgcccttgcc gcggtactgc cgcagcgtgc cgagcagggg gatacgggcg
      961 cggtcgatcc ggcgggagta ccacaggatc ttgaggaggc ccgggaagag ccggcccgag
     1021 ggattgtgct tcagggcgac gttgaagtcg gggatggcga cgccgaaccc caccacgcgg
     1081 ccctggatct cggcgaagac gacgagctcc gggatgatga tcggtttcag ctgtttggcg
     1141 agatgctcga gctccgcgtc ggtcatcggc acgtagcccc agttctgctc ccaggcctcg
     1201 ttgaacagcg ctttgacgag gtccacttcc tggcggaagc gcttcatgtc gagcgagcgc
     1261 agcgtcacgc cgagccgttc ggcgatgacc cggttcgccc gctcgaaccg ctcggggggc
     1321 gcctcgcctc ctccccagaa ggcgatcagg tccttcgcct tgtgaaatcc gtagcgctcg
     1381 tgcaggggca cgtagtacgc cgggttgtgc ggcatcagca ccaccggcgg ggtgtcgaag
     1441 ccctccacca gcagcccgca ctcgtcgttg atcgacgggt tcatgggacc gcgcatggtg
     1501 tcgaggccgt gctcgtgcag ccagcgcgcc gcggcgtcga acagggcgat cgcgggggcc
     1561 gggtcgtcga cgcactcgaa gaacccgaaa aacccgacgc gatcgtgatg cagccggttg
     1621 tgggcctcgt tccggatggc ggcgatgcgg ccgacgatgc ggccagcgtc ccgggcgatg
     1681 aaatactccg cctgggcgtg ctggaagaac ggattctttc gtggcgacaa gatggtgcgc
     1741 acgtccatcc gcaacggcgg gacccacatc gggtcccgcc ggtgcaacgt gtaggggaag
     1801 gcgatgaatc gttcgaggtc ccgtccggat cggacggggg ccacctcgac gctcaaatga
     1861 cctccagctc cttgccgagc cgggcgaagg tctcgatcgc gcggtcgatc tgctcgaagg
     1921 tatgggtggc catgaggctg gtgcgcagcc ggcactggga cggcggcacc gcgggcggca
     1981 cgacggggtt ggtgaacacc cctgcgtcga acagtttccg ccagaacagg aaggtctttt
     2041 cgagcgggcc gatgagcacc ggcacgatcg gggtctcggt ggggccgatg tcgaatccca
     2101 ggccgcgcag gccctgctgc agccggcggg tattgatcca gagccggtcg cgccgctcgg
     2161 gatcgcgctg cagcacgtgc agcgccgcca gcacgcccgc ggtgttcgcc gggggcagcg
     2221 ccgcggtgaa gatgagcggc cggctgtgat gcttgaggta atggatcacg gtctcgtcgc
     2281 cggccgcgaa tccgccgatg gacgccagcg acttggagaa cgtgccgacg atgaggtcga
     2341 tctcctcagt gagtccgaaa tgcgcggccg tcccgtcgcc cttggggccc agcacgccca
     2401 ccgagtgcgc gtcgtccacg gccagggcca cgccgtacct ctgcgccagc ttgatcaggt
     2461 tcggcagatc ggcgatgtcg ccttccatcg agtagacgcc atccacgatc accatcgtcc
     2521 cggtcccctc gggcaccttc tgcagcctgc gctcgaggcc ggccaggtca ccgtggttga
     2581 agcgcaccgt ttcgccctgg gacatcttgg cgccgtcgac gatcgacgcg tggtcgagct
     2641 tgtcgaggaa gacgacgtcg ccgcgaccga ccagtcccga gatgacgccc aggttggcct
     2701 gataaccggt cgagaagacg aggcaggatt ccttgccgaa gaactcggcc agctccgctt
     2761 ccagctgctc gtgcaggtcc agggtgccat tgaggaaccg gctgccggta cagcccgacc
     2821 cgtagcgctc cagcgccgcg cgggcggccg cgaggacctc cggatgatgc gtgagcccca
     2881 gatagttgtt ggagcccatc atgatccgtt ccttgccctc gatgacgacg accgtgtcct
     2941 cggagcgcga aatcggcttg aaataggggt acagcccggc tgcctggatt tcgcgcgcct
     3001 tggtgaagcc gcgacacttg tcgaagagcg cgatgtgctg cagggtctcg gtccgcacga
     3061 atctctcacc ttgtggaaat cgtgctacag cttagccgcg gcatgagcaa accgcaagga
     3121 gcatcatggt ttgcgcgttc gcgtagcgtc gctgctccac gtaacttacg ggctccgtgt
     3181 ttcactcctg caccggcgca cgccggctca cagtcctctg cgccggcctc gcgcttggcg
     3241 gcggctccct cgcggcgcag cacgcaccgc cgcgctacac cgccggttgg ggggatgtga
     3301 ttgcggtgtc cggtgccgga ctgctgacgg cagccggcgc gctggcgcac ccgccggcgg
     3361 ccgcctgcgc gccgtgcaac cccgcggact tgaccggttt cgatcgcggc gtgatcgact
     3421 ggaattccac cgccgcgcga cgcggcagcg acatcctgct cctgagtgtg gtgggcggcg
     3481 cggcgctcgc cagcgtcggc gggatggagc cggcgcgcgc gcgcggcgat gcggccgtac
     3541 tggcgaacgc gctgctctgg acgaccgccg cgaccgagtg gctcaaggtc ggcattcacc
     3601 gggcccggcc ggcgttgtac cgcagcggcg cggtcgaggc cgccgcggtg cacgagaatc
     3661 gtgagtcgtt cccgtcgggg cacgccgccg tggccttcgc cgcggcgacg gcctacgcca
     3721 cgctcgcggc gcgccagcac ctgccccatg cgacgcgcaa cgcgatcctg ctctacgccg
     3781 gggcggcgag cgtgagcgtg ttgcgcgtcg ccgggggcaa gcactttccc accgatgtgc
     3841 tcggcggggc ggcactcggc gcgggcatcg gctggacggt cgcgcggctg catcccatga
     3901 cccgatgagc gatcccggac acggcagcgg ccagccgccg cgcgtcatgg gactgcgcga
     3961 cgtcgtgctg ttcaacatca ccgcgatcgt cggcctgcgc tggctcacca ccgcctcgag
     4021 ccagttcggc ctggccgcgc tggcgctgtg gctggtggcg atgatcgtgt tcttcatccc
     4081 ctccggcatc gcggtgcgtg agctggccga catcgatccg gggcaggggg ggctgtaccg
     4141 ctgggtggct agagcgttcg ggaaccggca cgcgttcgtg gcggggtggg gctactgggt
     4201 caacaacctg ttctattttc cctcgctgct cgtcacgacg gcggcgatcg cggcgtacgc
     4261 gggcggcccg cgcaccgcac cgctgggcga agactcgacc ttcattctcg tggtgtcgct
     4321 gatcgggtta tggatcgccg tctggctcaa cctcgtcggc ctgcgggtgg ggaagtggct
     4381 gcagaacctc ggggggtggg ggacctggct ccccgccttg ttgttcgtgg ggctcgcggc
     4441 ctggtcgctg gtgcagcggg gcagcgcgac gccgttcacg gcacgcggcc tgcttccagc
     4501 ccgcctcgat ctgccgtcca tcaatctgtt cgcgacgatg acgttcgcct tcgccggtct
     4561 cgagctggcg ccgtcgctgg gcggggagat cgtcgatccg gcggccacac tgcgccgcgg
     4621 ggtgctgctg tcggggatcg cgatcgtggc gctgtacggg ctggggacgg tggcgatgct
     4681 ggtggcgttg ccgcacgaga ccgtgagcgt cacgaacggg atgccgcagg ccgtgcaggc
     4741 gctggccgac cgcctggcca tccccggtct gcggatcgcg gcgccgctca ccgccgtgct
     4801 cctcacgttc gggaatctgg gcggcgtcgg ggcgtggctc gcggggtcgg cgcggctgcc
     4861 gttcgcggcc ggggtggacc gggcgctgcc gccggccttc gcgcggctgc acccgcgctg
     4921 gcggaccccg tacgtgtccc tgttgctgat ggcggtgctg gccagcgcgt tcatcgtggc
     4981 gagcctggcc ggcaacaccg tgaagtcggc ctatctcctg ctcacgcaga ccacgctgat
     5041 tctcttcttc attccttatc tctacatctt cgccgcgtac ctgcggctgc gccgccgccg
     5101 cacggcagtc accctcctga cgggtctcct gggcctcgct gccgtggcgt tttcgatcgt
     5161 gctggggttc gtggtgccgg gtgacgaggc gcacccggat gcctaccggt tgaaggtggt
     5221 ggtcggagtg gtagtcttca tggggatcgg gttctggcta tcgcggcgta gcgtgagggg
     5281 gcccacagcc acggccgcgt ccgcaccata aggtgatccg ctccggagga caccgcatgc
     5341 cgcttgttcg ttcgtacttc ctggtgtgcg ccgcgtcgtg cctggcgagc gtcgccgggg
     5401 cgcagggtcg tcccgccgcc gcgcgacagc ggcccgggcc gcgctgggag atcccggggt
     5461 tggacatcac gccgaacggc ggctggcgcg tgcgggcgcg cgcggtcgcc gccacccggg
     5521 cccgtctgct ggcccagcgc aacttcgccc tgctcaacgc ggcgccggtc ggccccacgg
     5581 cggtgaacgc ggtcaccgga gcgctgcgcg ttccggtggt gatgttccgc taccaggaca
     5641 gccccgcggg ccagtactca cgcgacacga cccagtacaa cgcgacgctg ttcgggaccg
     5701 tgccgccggc gggcaggcca tacacattgc gcagcttcta cgagcagctg tcgaatgggc
     5761 tgttcagcat ccagggcgcc gcggccgggt gggaagcgct ggccggcaac gagagcgcct
     5821 acaccggcgt cgcgggcacc tgtcccggca acccctacgg gaccaacaac tgcaacggga
     5881 tatggagcac cgcggcgttc acggcgatgc tgagcgggtt gcaggaggcg ttgacccatg
     5941 ccgactcgac gatcgatttc ggccagtacg acaacgacgg gccggacggc attccgaatt
     6001 ccgatgatga cgacggggtc gtggacgcgg tgctgttcct gcatcccagc ctggacggcg
     6061 cctgtctgag cgccacgaac aatcacctgt ggtcgcatcg cttcagcctg aacgacttcg
     6121 ccaccaacga tgccgcgacg ccgggaccga agaagacgtc gtcgcatgtg ctcgtgcgcg
     6181 actacatcct ccagagcggc ctcggcagct cgctccccag tgggagcccc tgcggcacgg
     6241 atgcgattat gccgatcggc accgcggcgc acgagctggg gcacatcctg gcgctgcccg
     6301 acctctacga cgtcagcggg tccaccgaag ggatcggcga attcggcctg atgggaagcg
     6361 gtaactacac caccggcaac agcccgtcgc gctacgacgc ctggtcgctg cagcagatgg
     6421 gctggaccac gattcagccg ctcacgacga ccgggaccta cacggtgggt cccgtcccca
     6481 ccgcggacac cgtgttctac gtgaacgtgc agggcgcgaa ccccgacggc gagtacttcc
     6541 tgatcgagaa ccggcaggag gtgctgtccg acagcgccat gctgagagtc cattgcgcgc
     6601 tgagcggcga tgccccgggc tgcgcgggag gcttcctcat ctatcacgtg gacgggctga
     6661 aggcctgcat gatcaacgtg tgcggcaaca gcgtgaacgc cggctcgatt cacggggtgg
     6721 cgatcgagga ggccgacgga ctgcgcaacc tgtggaacgg ggacaatcgc ggcgacgggg
     6781 gagatctcta tcccggcacg accgacaaca ccgccctcgc attccgcacg agccccgcgg
     6841 ccacgaagaa caccgatacg agcttcgcgg gcttcgccgt cgattcgatc cggcagctgg
     6901 tgccgaacgg cgcgatggcg ttccggttgc gtttcggcag cctgacatcg gtggccgcga
     6961 acgacacggc ggccgaggtg caggtggacg ggcagccata caacgtattc caggatctgt
     7021 tcgacgaggg gtcgagccat acgatcgcgg tggcggactc gcagctctcc acgagcgggc
     7081 ggacgcgact caccttccag tcgtggtccg acgggggcgc gatcagtcac gtgatcaccg
     7141 gcgccgcggc cggcgccacc tacacggcga cgctcgcccg cgagcaccgg ctggatgtca
     7201 ccgtggggac gaacggtgcc gtggcgtaca gtcctgtggc ggattcgagc gggagtttca
     7261 tcctccaggg cacacccgtg accctcacgg ccacgcccac tgcgcccttc gtgttcggtg
     7321 gctggaccgg cgatacgacg gccgcgggcg cgctgctcgt gctcccgatg ggccggccct
     7381 ttgcg
//
LOCUS       DBNGZW010000558         5610 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_597768, whole genome shotgun sequence.
ACCESSION   DBNGZW010000558 DBNGZW010000000
VERSION     DBNGZW010000558.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_597768"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            289..858
                     /locus_tag="AC16JC_13250"
     CDS             289..858
                     /locus_tag="AC16JC_13250"
                     /inference="COORDINATES: protein motif:HMM:NF024966.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybH family protein"
                     /protein_id="HIR5165710.1"
                     /translation="MQPRPLPSIVVPLLHLEARLRLSAWLGTTLLALAACKPAATPPP
                     DTSAAAKAAIDAANATWPRLTSSGHSDSLADLYTADAVMMPPNMATVQGRDSIRAFFA
                     VMNTMAGVTLTLRAESVWASGPAATEMGRWTWSWAAGVKPPPGMPAADSGKYLVRWVE
                     ENGQWRMAQDIWNSDLPLPTPPAPTPARR"
     gene            complement(919..1449)
                     /locus_tag="AC16JC_13255"
     CDS             complement(919..1449)
                     /locus_tag="AC16JC_13255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165711.1"
                     /translation="MPLALAGSLLLVGALPTRGLAQDCSGTTFFVSAVVVAGLAVWDI
                     ASTPRSVRRYNERHVAVAPVVNVRQHRYGVSLVVPLGRRRAPPLAERRVAAAGPRSAT
                     TGVLLAVGATGVPIGTGLLMGADAGAWVFLSGVVVGPSVGQFYAGQTARAAGTIALRA
                     AGSAVGISSLVGCFSD"
     gene            complement(1476..3086)
                     /locus_tag="AC16JC_13260"
     CDS             complement(1476..3086)
                     /locus_tag="AC16JC_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165712.1"
                     /translation="MRRPARRLVIVPLVLLAAPLAAQEHVHPGAERLGYVEFPVSCNA
                     QARQGFERAMALLHSFWWGEAERAFQSVAAADSTCAMAYWGLALTRWRNPIAGGPGAA
                     DLRAGQEAARRAAALGGRTPRERDFIAAVGALYGDTGTVPNSVRLRRYADAMERVYAR
                     NPRDPEAGIFFAIALTASAPAGDTTFARQRRAAAILNPLFRAHPDHPGLAHYLIHAND
                     SPQLASLGLEAARRYAAIAPSAPHAQHMPAHIFVRLGLWDETVRSNRASYQADVAAGG
                     ERQALTADQLHAMDYMVYAYLQLGQDTAAAAVMAEGQADTSAAPAQAMVAAYNRNAMA
                     ARFALERGQWAEAARLPLRPTPGTPMAEMVTRFARGIGAVRGGDTAEARAEIAALAAI
                     EAELERRGDPYWPRVTRVKQRAIAAWIALAAGDTAGALVGAHAAADSEDVVGKHAVTP
                     GEVVPARELEADLLLGLGRAAEARRAYARVLALEPHRARSVFGAARAAELAGDRSGAA
                     AGYREYVRLMERADGARAELAVARAFVP"
     gene            3272..>5610
                     /locus_tag="AC16JC_13265"
     CDS             3272..>5610
                     /locus_tag="AC16JC_13265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165713.1"
                     /translation="MARRLGTCFIALFGLFFNDIRGQDTLPPTQGGYVHSLGLPAIYK
                     PFFGVGLGLSRNRTAHLAAQFRTGVFKDIGSPITELVGWSVEGFAGVRDLDADYGARA
                     FLLSHLLGVGAGLEYDVRDGRGSPLLLLEAPMRRGGLLGAGSDLRLEWLSGRGASFNL
                     AVSFPVGQPHRGRTRPMRDFVQLTAERPRPLAFVPADSVLRDALAALRVHALWINRFT
                     VPDLGSLAADPARAATDAVAPLKAHLAAGSVDAEIRGYHHEMVRAFSIAAAGTPSSGD
                     STPQGAAAAASATAILLDRVIFPYNRLIGQPKKRDSTRDFGSHARGVFARWLTMSSSV
                     SPERDEACLYVFQHLLDVVEEIRAVNRRTWSNSALVWLPLQLALVPEEYDEQTELDSI
                     ISRAVGHSVTHGNRIWYVLNERFQRELEKSIANAEDYHILWIHDFRGLNDSGKPDRLS
                     LLTATQAYLAAMRHRLATYDSTGRLPVYMIFLDQHYFEKFKSRDLLRFLEDPLGRRPG
                     LPEGFESLARELASAQQQLWHAVNASRLLTAERAQYGESWLHRLMKVHVSVTNPADPA
                     FRSRQMLPLVGIPDDMMRDHRKAVLYDVSEADPYRGMAMYAGMGVGEHYVGPTWEDRA
                     IMLQGPVALTLRDEARALLESQDLRGGNVPHVLRPSRRAPDYDRRVKAEIDSMDAWGG
                     VATRAIELENETGFGRKEVSVADATLFSLLAPGSVMKVPDSLWLNQLLASLLVGSALR
                     GARVLVIAPSRASAPGKGWPTLALMHDLMSRLVALQHALA"
BASE COUNT          852 a         2113 c         1851 g          794 t
ORIGIN      
        1 ccattcaggt gcgctaccgg actgcgctac gtcccgatgc aacgaacttc tacaaccgga
       61 ccggaacagc taccggactg cgacgcgagg atcgcccgca gggcgatccg agctagtcct
      121 ttagggcgct acgtcccggg actgctagtc gctcaggtgc ggagatgctc agtcgcatag
      181 aaaaggccct taccagaacc accctgcgtc ggagcgaccc ggcggagcga agataaccga
      241 accttcgtcg caggcacaac gtcagaccgt gatgaatcag gtcatccctt gcagccccgc
      301 cccctgccct ctatcgtcgt ccccctcctc cacctcgagg cccgcctgcg cctgtctgcc
      361 tggctcggca ccacccttct cgccctggcc gcctgcaagc ccgccgcgac gccgccgccc
      421 gatacctcgg cggccgccaa agccgccatc gatgccgcca acgccacctg gccacggctc
      481 acgtcctccg gccactccga ttcactcgcc gatctctaca cggccgacgc cgtgatgatg
      541 ccccccaaca tggcgaccgt ccaaggccgc gactcgatcc gcgcgttctt cgccgtcatg
      601 aacacgatgg ccggcgtgac cctcaccctc cgcgccgagt cggtgtgggc gagcggcccc
      661 gccgccaccg agatgggccg ctggacctgg agctgggccg cgggcgtcaa accgcccccc
      721 ggtatgccgg cggcggactc gggcaagtac ctcgtccgct gggtggagga gaacggacag
      781 tggcgcatgg cgcaggatat ctggaacagc gacctgccac tgccgacgcc accggctccc
      841 acgcccgcgc ggcgctgata ccgtctcgcc ctcgcgaaac gacaagcccc ggcgatgagc
      901 cggggctctc ttacactctc aatcgctgaa acaccccacg agcgacgaga tccccaccgc
      961 actcccggcc gcccgcaacg caatcgtccc cgccgcacga gcggtctgtc cggcgtagaa
     1021 ctgccccacg ctggggccca ccacgacccc gctcagaaac acccaggccc cggcgtcggc
     1081 gcccatcagc agacccgtcc cgatcggcac accggtcgcc ccgaccgcca gcagcacgcc
     1141 ggtcgtcgcg gacctgggcc ccgcggcggc gacgcgtcgc tccgccagcg gcggcgcgcg
     1201 cctccgcccg agcggcacca ccagcgacac tccatagcgg tgctgccgca cattcacgac
     1261 gggagccacc gccacgtgcc gctcgttata acggcgcacc gagcgcgggg tcgaggcgat
     1321 gtcccacacg gccaggccgg ccacgacgac cgccgaaacg aagaacgtcg tgccgctgca
     1381 atcctgcgcg aggccccgag tcggcagcgc tcccacgagc aggagcgagc cggccagcgc
     1441 cagcggcaat cgggatccgg atttcgcgcc gacgatcacg gaacgaacgc ccgggccaca
     1501 gccagctcgg cccgcgcgcc gtccgcccgc tccatcagcc gcacatactc gcggtacccc
     1561 gcggctgccc cgctgcgatc gcccgccagc tccgccgccc gcgccgcgcc gaatacgctg
     1621 cgggcccggt gcggctcgag cgccagcacc cgcgcgtacg cccgtcgcgc ctcggcggcg
     1681 cggcccagcc cgaggagcag gtcggcctcc agctcgcgcg ccggcaccac ctcacccggc
     1741 gtcaccgcgt gtttgcccac gacgtcctcg gagtcagcgg cggcgtgggc tcccacgagg
     1801 gcaccggccg tgtcgcccgc ggcgagcgcg atccacgcgg cgatcgcgcg ctgcttgacg
     1861 cgggtcaccc ggggccagta cggatcccca cgacgctcga gctccgcttc gatcgcggcc
     1921 agcgccgcaa tctcggcgcg cgcctcggcc gtgtcgcccc cccgcacagc cccgatgccc
     1981 cgcgcgaagc gggtgaccat ctcggccatc ggtgtcccgg gcgtcggccg cagcggcagg
     2041 cgcgcagcct cggcccactg tccccgctcg agcgcgaacc gcgccgccat ggcgttacgg
     2101 ttgtacgcgg cgaccatcgc ctgggccggc gccgccgacg tgtccgcctg cccctccgcc
     2161 atgaccgccg ccgctgccgt atcctgtccc agttgcagat aggcgtacac catgtagtcc
     2221 atcgcgtgca gctggtcggc ggtcagcgcc tgccgctccc cgcccgcggc cacgtccgcc
     2281 tgatacgacg cccgattgga gcgcaccgtc tcgtcccaca ggccgagccg cacgaagatg
     2341 tgcgccggca tgtgctgggc gtgcggcgcc gacggcgcga tcgcggcgta gcggcgcgcc
     2401 gcctccagcc cgagcgacgc gagctgcggc gaatcgttcg catgaatgag gtagtgggcc
     2461 agtccggggt ggtccggatg cgcccggaac agggggttga ggatcgccgc cgcacgccgc
     2521 tgccgcgcaa acgtggtatc gcccgccggg gcggacgccg tcagcgcgat cgcgaagaag
     2581 atgccggctt cggggtcccg tgggttgcgc gcgtacactc gctccatggc gtcggcgtag
     2641 cggcgcaggc gcacgctgtt gggaacggtg ccggtgtccc cgtacagcgc gcccaccgcc
     2701 gcgatgaagt cgcgctcgcg gggcgtccgc ccgccgagcg ccgccgcgcg ccgggccgcc
     2761 tcctgccccg cccgcaggtc ggccgcgccc ggacccccgg cgatcgggtt gcgccaccgt
     2821 gtcagcgcca acccccagta cgccatcgcg caggtcgagt ccgccgcggc cacactctgg
     2881 aaggcgcgct cggcttctcc ccaccagaac gaatggagca gcgccatggc gcgctcgaac
     2941 ccctggcggg cctgggcgtt gcaggaaacg gggaactcga cgtaccccag ccgttctgcg
     3001 ccggggtgca cgtgttcctg cgccgcgagc ggcgcggcga gcaggacgag cgggacgatg
     3061 acgaggcgac gagcagggcg gcgcatggag cagctcacga aggctggagt cgggagtgtg
     3121 ggagactccg atagaatact cgagaacggg gcggcggtct gcccccccgt acaccggtag
     3181 ctgccccgcc ctgagccggc cgtttcctct gtagttcatg cccgcttcag agtcatcgcg
     3241 ataagaacac ggtggaccca ccgggaggac cgtggcacgc cggctcggaa cgtgctttat
     3301 cgcgctgttc gggctgttct tcaacgacat tcgtggccag gacaccttgc cccccacgca
     3361 gggcgggtac gtccactccc tcggcctgcc ggcgatctat aagccgttct tcggagtcgg
     3421 gctcggcctc tcgcgcaacc gcacggccca cctcgcggca caattccgga ccggcgtgtt
     3481 caaggacatc ggcagcccga ttaccgagct cgtcggctgg agtgtcgaag ggttcgccgg
     3541 cgtgcgcgac ctcgacgccg actacggcgc ccgggcattt ctgctcagcc acctgctcgg
     3601 tgttggtgcc ggtctcgaat acgacgtgcg cgatggccgc ggctccccgc tgctgctcct
     3661 ggaggcacct atgcgtcgcg gtggcctgct cggggccggc agcgacctgc gcctcgagtg
     3721 gctgtcaggt cgcggcgcgt ccttcaacct ggccgtaagc ttcccggtgg ggcagccgca
     3781 ccggggccgc acgcgtccca tgcgcgactt cgtgcaactg acggccgagc ggccacgtcc
     3841 gctcgcgttc gtgccggccg actccgtgct gcgcgatgcg ctggcggcgc tgcgtgtgca
     3901 cgcgctctgg atcaatcggt tcacggtgcc ggacctgggt agcctggcgg cggatcccgc
     3961 gcgcgcagcg acggacgccg tggctcccct caaggcgcac ctcgccgccg ggagcgtaga
     4021 tgcggagatc cgcggctatc accacgagat ggtccgtgca ttttccatcg ccgcggcggg
     4081 aacgccgtcg tcgggcgact cgacgcccca gggcgccgcg gcggcggcca gcgccaccgc
     4141 gattctcctg gaccgggtga tcttcccgta caaccggctc atcggacaac cgaagaagcg
     4201 cgacagcacc cgggacttcg ggagtcacgc ccgcggcgtg tttgcgcgct ggctgaccat
     4261 gtcgtccagc gtgagcccag agcgtgacga ggcgtgtctg tacgtgttcc agcacctgct
     4321 cgacgtcgtg gaggagattc gggcggtcaa tcgccgcacg tggagcaact ccgccctggt
     4381 gtggctcccg ttgcagctcg ccctcgtgcc ggaggaatac gacgagcaaa cggagctcga
     4441 ctcgatcatc agccgggccg tgggacactc tgtcacgcac ggcaaccgga tctggtatgt
     4501 gctgaacgag cggttccagc gcgagctcga gaagtcgatc gcgaatgccg aggactacca
     4561 catcctctgg atccacgatt tccggggcct gaacgattcg ggcaagcccg accggctgtc
     4621 gctgctcacg gcgacccagg cctacctcgc ggcaatgcgc caccgcctgg caacctacga
     4681 ttccacgggg cggctgccgg tctacatgat cttcctcgac cagcactatt tcgagaagtt
     4741 caagagccgc gacctcctgc gctttctcga ggatcccctg gggcggcgcc cgggactgcc
     4801 ggagggattc gagtcgctgg cgcgcgagct ggcctcggcg cagcagcagc tgtggcatgc
     4861 cgtcaacgcc tcccgcctgc tcacggcgga gcgggcccag tatggcgagt cgtggctgca
     4921 ccgcctcatg aaagtgcacg tcagcgtcac caacccggcg gaccccgcgt ttcgcagccg
     4981 ccagatgctc ccactggtcg gcattccgga cgacatgatg cgcgatcatc gcaaggctgt
     5041 cctgtacgac gtcagcgaag cggatcccta ccgcggcatg gcgatgtacg ccggcatggg
     5101 cgtgggcgag cactacgtgg ggcccacctg ggaagaccgg gccatcatgc tgcagggacc
     5161 ggtcgcgctg acgctgcgcg acgaagcccg cgcgctgctc gaatcgcagg accttcgcgg
     5221 cggcaacgtg ccccacgtac tgcgccccag ccgcagggct cccgactacg accgcagagt
     5281 gaaggcggag atcgacagca tggatgcctg gggcggcgtg gcgacccggg ccatcgagct
     5341 ggaaaacgaa accggattcg gtcgcaagga agtcagcgtc gccgacgcca cgctgttcag
     5401 tctcctggcg ccggggtcgg tgatgaaggt cccggactcg ctatggctca accagctgct
     5461 cgcctccctg ctggtcggca gcgcgctacg cggcgcgcgg gtgctggtca tcgcaccatc
     5521 gcgggcgtcg gcgccaggca agggatggcc caccctggcc ttgatgcacg acttgatgtc
     5581 gcgcctcgtg gcgctgcaac acgccctcgc
//
LOCUS       DBNGZW010000559         3017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_937759, whole genome shotgun sequence.
ACCESSION   DBNGZW010000559 DBNGZW010000000
VERSION     DBNGZW010000559.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3017
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_937759"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..605)
                     /locus_tag="AC16JC_13270"
     CDS             complement(180..605)
                     /locus_tag="AC16JC_13270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165714.1"
                     /translation="MKRILLLAGFASLFAAGTASAQTSVELSLGFGVPRPYISGDVFI
                     GRSFIRPVYRPFYYYYPSRYPYYFYEPLPRWYGPPQRVIIYERDHRFYRRDFDRDRDW
                     ERRYSRHEGRHRGWNRAYGRERDRDFDRDRRHRDRDDER"
     gene            complement(753..1820)
                     /locus_tag="AC16JC_13275"
     CDS             complement(753..1820)
                     /locus_tag="AC16JC_13275"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR5165715.1"
                     /translation="MKIAISTGGGDAPGLNAVIRGAVLSAINRGWQCVGVRRGFGGLL
                     GEDLLIPLGASEVRGITHMGGTILGTTNRGNPFNWREQLPDGGWSERDRSDELVTAFR
                     AFELDALVVIGGDGTLDIARRLMAKGIPIVGVPKTIDNDVGGTVATFGFDTAVSTATD
                     AIDKLHSTAESHERVMVVEVMGRHAGFIALNSGIAGTADVILVPEIPFDINRVCDKIR
                     EREAHGRHFSIVVVAEGAAPAGGQESYLDPDRRRLGGIAEKVAEDIERITGKETRSLV
                     LGHLQRGGSPTTFDRLLGLRFGAAAVRLVARRQFGMMVALQPPDIVPVPIDQVLSRPK
                     RIPLDSDVIATAREIGISFGD"
     gene            complement(1817..2053)
                     /locus_tag="AC16JC_13280"
     CDS             complement(1817..2053)
                     /locus_tag="AC16JC_13280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(2021..2023),aa:Sec)
                     /transl_table=11
                     /product="SelT/SelW/SelH family (seleno)protein"
                     /protein_id="HIR5165716.1"
                     /translation="MRVTIEYCVVUNYEARAAGLAAEIRRTFPDAEIKLIESSGGVFE
                     VEADGRSIFSKKTSKRHAEPGEVVRLLQEAGTGS"
     gene            2168..>3017
                     /locus_tag="AC16JC_13285"
     CDS             2168..>3017
                     /locus_tag="AC16JC_13285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165717.1"
                     /translation="MPTTLVPVVVALGALAAIGVAFAGYASARLATGGAFALTFTGLV
                     VIGALTRRFGIPLPGNGFSSYVLGVTTFAVLERGWEFAAVVAPLLTVLGDVVLRRLPL
                     RIAGSNAAHLTAGSALVGIIYERLGGGTGSLALEPDNIGPIAALLVLMMVIVNGSFYL
                     ELAMSRSVAWVDARLTARWEAIVYATSVGLALLWFRLFHTALPVAAWILVAAALLGAT
                     LVSAHVIRLGVHADQLLLVQRLARTIVTDLNLTRTFAHIQETTRRLVPWEHIGFARFD
                     AKRREME"
BASE COUNT          458 a         1030 c         1026 g          503 t
ORIGIN      
        1 gtcggtgcga tcacgatgac gcggccggtc gccggctccg ccgcgaggaa cgcctgggga
       61 tcggggggga ggaagtggtc gcgggttgga ggcacaggtc aattcaaaga ggcaaccgcg
      121 aaaaaagcaa acggccggga gccgctgggg cccccggccg cctgctgttg tctcatgact
      181 cagcgttcgt cgtcgcggtc gcgatggcgc cgatcccggt cgaagtcgcg atcacgctcg
      241 cggccgtagg cccggttcca accgcggtgc cggccttcgt gtcggctata gcgccgctcc
      301 cagtcgcggt cgcggtcgaa gtcacgacga tagaagcggt gatcgcgctc gtagatgatc
      361 acgcgttggg gcggaccgta ccatcgcggc agcggctcat agaagtagta cgggtaccgg
      421 ctcgggtagt agtagtagaa cggccggtac accggtcgaa tgaacgaccg gccgataaac
      481 acgtcgcccg atatgtacgg gcgcggcacc ccgaacccaa gagacaattc gacgctcgtt
      541 tgagcggagg cggtaccggc cgcgaagagc gaggcaaacc cggcgagcag caagatccgt
      601 ttcatggctt cctcctgtga gtcggccgct gtacagtgga agccccgtgc caccagggga
      661 tgaggccgca agccgttggc gccacgtcgg ttctggtgga aggaccagga aggcgctgcc
      721 gcggcgtgtc ccctcccgag gtcagcggga ggtcaatcgc cgaagctgat gccgatctcc
      781 ctggcggtgg cgatgacgtc ggaatcgagg gggatccgtt tgggacgcga gagcacctgg
      841 tcgatgggaa cggggacgat gtccggcggc tgtagcgcca ccatcatgcc gaactgccgc
      901 cgggcgacga gccgcacggc cgcggcgccg aagcgcagcc ccagcagccg atcgaacgtc
      961 gtggggctac ctccccgctg cagatgtccc agcaccagcg agcgggtctc cttgccggtg
     1021 atccgctcga tgtcctcggc gaccttctcg gcgatgccgc cgaggcggcg acgatcggga
     1081 tcgagataac tctcctgccc accggcgggg gccgctcctt cggccacgac cacgatgctg
     1141 aagtgacgac cgtgcgcctc ccgctcgcgg atcttgtcgc acacgcgatt gatgtcgaac
     1201 gggatttcgg gcacgagaat gacgtcggcg gtgccggcaa tcccggaatt cagggcgatg
     1261 aagccggcat ggcgtcccat cacctcgacc accatcaccc gctcgtggct ctcggcagtc
     1321 gagtggagct tgtcgatggc gtcggtggcg gtggagaccg ccgtatcgaa tccgaaggtg
     1381 gcgacggtgc cgccgacgtc gttgtcgatg gtcttcggga ctccgacgat gggaatgccc
     1441 ttggccatga ggcggcgcgc gatgtcgagg gtcccgtcgc cgccgatcac cacgagggcg
     1501 tccagctcga aggcccggaa cgcggtgacc agctcgtcgg agcgatcccg ttcgctccag
     1561 ccgccgtcgg ggagctgctc gcgccagttg aaggggttgc cgcggttggt cgtaccgagg
     1621 atggtgccgc ccatgtgggt gatgccccgg acctcgctgg cgcccaacgg gatgagcagg
     1681 tcctctccca gcaggccgcc gaagccgcgt cgcactccca cgcactgcca tccccggttg
     1741 atggccgaga gcactgctcc acggatgacg gcgttcagcc ccggcgcgtc cccaccgccc
     1801 gtcgagatag cgatcttcac gacccggtgc cggcctcctg cagcagacgg acaacctcac
     1861 ccggttcggc gtggcgcttc gacgtcttct tcgagaagat gctccgtccg tccgcttcca
     1921 cctcgaacac gccgccggag gattcgatca gcttgatctc cgcgtccggg aaggtccgac
     1981 ggatctcggc cgccagaccg gcggcccgcg cctcgtaatt tcagacgacg cagtattcta
     2041 tcgtgacgcg catggccgga acgatagcgg cgcttgacat gctgcgccac acggcagagc
     2101 ttgcccgcat ccctctctcg cgcaggatgc cgcggccggc atgagtccgc tcccgcgttc
     2161 ccgagcggtg cccacgaccc tggtcccggt cgtcgtcgcg ctgggcgccc tcgccgcaat
     2221 cggtgtggcg ttcgccggat atgcgagcgc gcgactcgcc accggcgggg ccttcgcgct
     2281 gaccttcacc ggcctggtag tgatcggggc gctcacccga cgcttcggca ttccgctgcc
     2341 cggcaacggc ttctcctcct acgtcctggg cgtgaccacc ttcgccgtgc tcgagcgcgg
     2401 ctgggagttc gcggccgtcg tcgcgcccct gctgaccgtc ctgggcgacg tcgtgctccg
     2461 tcggctgccg ctgcgcatcg ccggctcgaa cgccgcccac ctcacggccg gctcggcact
     2521 ggtgggtatc atctacgagc ggctgggcgg cgggacgggc agtctcgcgc tcgagccgga
     2581 caacatcgga cccatcgcgg cgctcctcgt cctgatgatg gtgatcgtca acggctcgtt
     2641 ctatctcgag ctcgcgatga gccgctccgt cgcgtgggtg gacgcccgcc tcaccgcccg
     2701 gtgggaggcg attgtctacg ccacctcggt cgggctcgcc ctgctctggt tccgcctgtt
     2761 tcacacggcc ctgccggtgg ccgcgtggat cctggtggcg gcggcgttgc tgggcgccac
     2821 cctcgtctcg gcccacgtga ttcgactggg cgtgcatgcc gaccagctat tgctcgtgca
     2881 gcggctggcg cgcaccatcg tgaccgacct caacctgacg cgcaccttcg cccatatcca
     2941 ggagaccacg cgccgtctcg tgccctggga gcatatcggg ttcgcacgat tcgacgcgaa
     3001 gcgacgggag atggagc
//
LOCUS       DBNGZW010000560         4544 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_256822, whole genome shotgun sequence.
ACCESSION   DBNGZW010000560 DBNGZW010000000
VERSION     DBNGZW010000560.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4544)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_256822"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1696)
                     /locus_tag="AC16JC_13290"
     CDS             complement(<1..1696)
                     /locus_tag="AC16JC_13290"
                     /inference="COORDINATES: protein motif:HMM:NF036516.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M61 family metallopeptidase"
                     /protein_id="HIR5165718.1"
                     /translation="MSRTTAVLTLLLSVAARGHAQNPLSRPVDAVEVRYSTAQPVVRY
                     TLRVDSADRSGFDVELRIRNARDTIRLAMVAHPEYDDRYWRFVRDVRVEGATGAGSAA
                     REDSAVWRVAIPGGAGVVRSRLQLPPAEGGFRSAWVPFLAPTGGLVGGPHSFLYVVGA
                     ELAPAYVTLDLPGGWDVATGLEPTADPATFFAASVGVLVDSPILLGRLRSWRFAVDGV
                     PHRVVYWPLPDAQPFDTAALVGGLERFARQAVALFGRAPYRDFTFQIQDGAVGSLEHA
                     NSVSIGAPSARLSQGLTEFFAEAAHEYFHTWNLMRIHPVEYGGVDYRTPPRSRGLWWA
                     EGITILYADLLSRRARVPLFDSTRSAHVEGAIARYVANPGNARFSAESVSVVAYGTQP
                     GALGDYSASTHLQGELLGTMLDLVVRDATGGRRSLDDVVRATLERFSGERGYTGRDLE
                     RTVADVCGCVVRDFFDRYVRGANPIDFDRYLALIGLRTAVAWIPATGPDGRPAADLRV
                     FAWLPEGGRALRLRVWNPASVWNRAGLHTGDRIVTVNGAPLAGMADFRRVFWSLRIG"
     gene            1777..2526
                     /locus_tag="AC16JC_13295"
     CDS             1777..2526
                     /locus_tag="AC16JC_13295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR5165719.1"
                     /translation="MNPGQDHFSGVAASYAEYRPRYPTELVDLLADLAPHRDVAWDAG
                     CGSGQLSVALAERFARVIATDASEQQLAQATTHPRVEYRRASAEASGIPSATVDLATA
                     AQAVHWFDRPAYYQEVRRVCRPGALIALVTYNLFRITPELDRIIDHFYWQTIWQYWPP
                     ERRLVEAGYRTLDFPFAELPAPPLEMQAEWTLPHVLGFVSTWSALRGLERDRGGRVVE
                     EFARELSGAWGAAQTPRPVRWELAVRLGRVS"
     gene            2606..2812
                     /locus_tag="AC16JC_13300"
     CDS             2606..2812
                     /locus_tag="AC16JC_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165720.1"
                     /translation="MTWAVQAGIAAALAMGATLARGRGTTLLLAGMAGGAAIASVLGL
                     WMRPPEALARPQRDGTTSISTLEG"
     gene            complement(2770..>4544)
                     /locus_tag="AC16JC_13305"
     CDS             complement(2770..>4544)
                     /locus_tag="AC16JC_13305"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010038432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR5165721.1"
                     /translation="VAQAQQPAPDSAFVTTDTMIPMRDGLRLNTIIVAPRDTLPHPIL
                     MQRTPYGAAGPAAGVPQAAAMLGLDGYILVFQDIRGRFKSEGAFDMNRPPRSGHAGTD
                     ESTDTYDTIDWLVKHVPSNNGRVGIFGISYPGWLTAVGGIGAHPALKAISPQAPMGDT
                     WMGDDFFHQGAFRLTYGLEYAWMMEASPDLSVTPSPARYDTYEWYRSFPTLGALAAAV
                     GADRWPTWRRFVAHPAYDSAWQARSLPRYFTHTTVPTLTVGGWWDQEDEYGPLASYAA
                     LERSDNAAKNFLVMGPWYHGQWFADSGIALGNIRFDRSTGADFRALQSRWFAYWLKGQ
                     GDGKFDEATVFDAGTNEWRTFDRWPPAGAAHRRLYFQADGGLSFRPPTAAAGFDQYVS
                     DPARPVPYRPRPVERTYSPTSRWRRWETEDQRFVDGRTDVLTWQTAPLGADVTVAGNV
                     VARLFASTTGSDADWVVKLIDVYPDTVPDRPTMGGYELMVTGEIMRGRYRAGWDHPTR
                     IPPSAVTPFTVDLHQQSYTFRKGHRIMVQVQSTWFPLYDRNPQTFVPNIFTARAEDYR
                     AQIHRVYRTARYPSSVEIDVVPSR"
BASE COUNT          690 a         1531 c         1665 g          658 t
ORIGIN      
        1 cgccgatgcg caggctccag aacacccggc ggaagtccgc catgcccgcg agcggcgcgc
       61 cgttcaccgt gacgatccga tcgccggtgt gcaggcccgc gcggttccac acgctcgccg
      121 ggttccagac ccgcaggcgc aacgcgcgac caccctccgg gagccaggcg aagacgcgca
      181 ggtcggcggc cggccggccg tcgggacccg tcgcgggtat ccaggcgacc gcggtccgca
      241 ggccgatcag ggcgaggtag cggtcgaaat cgatcgggtt ggcgccccgc acgtaccgat
      301 cgaaaaagtc ccggaccacg cacccgcaga cgtcggccac ggtgcgctcg aggtcccgcc
      361 cggtgtagcc gcgctcgccg gaaaaccgct ccagcgtggc gcgcaccacg tcgtccagcg
      421 agcgccgtcc gccggtggcg tcgcggacca cgagatcgag catcgtcccg agcagctcgc
      481 cctggaggtg cgtgctcgcg ctgtaatcgc ccagcgcgcc gggctgcgtc ccgtaggcga
      541 cgacgctcac actttcggcg gagaagcgcg cgttgcccgg attggcgacg tagcgcgcga
      601 tcgcgccttc gacgtgcgcg ctgcgggtgg aatcgaacag gggcacgcgg gcccgtcgcg
      661 acagcaggtc cgcgtagagg atcgtgattc cctcggccca ccacagcccg cgggagcgcg
      721 gcggcgtgcg gtagtccacc ccgccgtact cgaccggatg aatgcgcatg aggttccacg
      781 tgtggaagta ctcgtgcgcc gcttcggcga aaaactccgt cagaccctgc gacagccgcg
      841 cgctgggggc gccgatcgag accgaattgg cgtgctcgag cgatccgacc gcgccgtcct
      901 ggatctggaa ggtgaagtcc cgatacggcg cgcggccgaa cagcgccacc gcctgccgcg
      961 cgaaccgctc gagaccaccg accagcgcgg cggtgtcgaa cggctgagca tccggcagcg
     1021 gccagtagac gacccggtgc gggacgccgt ccaccgcgaa gcgccagctg cggagccggc
     1081 ccaggaggat cggggagtcc accagcacgc ccacactcgc ggcgaagaac gtcgcggggt
     1141 cggcggtggg ctcgaggccc gtcgccacgt cccatccgcc gggcaggtcg agcgttacgt
     1201 acgcgggagc cagctcggcg ccgaccacgt acaggaagga gtgcggtccc ccgaccagtc
     1261 ctcccgtggg cgcgagaaac ggcacccacg cggagcgaaa cccgccctcg gccggaggga
     1321 gctgcagccg ggaacgcacg accccggctc caccgggaat cgcgacgcgc cacaccgcgc
     1381 tgtcctcccg cgccgcggag ccggctccgg tcgcgccctc gacccggacg tcgcgcacga
     1441 atcgccaata gcggtcgtcg tattccgggt gcgccaccat cgccagccgg atcgtgtcac
     1501 gcgcgttgcg gattcgcagc tcgacgtcga acccggagcg atcggcactg tccacccgga
     1561 gcgtgtagcg gacgaccggc tgggcggtgg aatagcggac ctcgacggcg tcgaccggac
     1621 gggacagggg attctgggcg tgcccgcggg cggcgacgga gagcagcagg gtcaggacgg
     1681 cggtggtgcg cgacatggag ggctcgtgtg ggtacatggc tacgatgccg ctcacgcgcg
     1741 tcgggtttga gcgggggagc cgatagattc cattgcatga atcccggcca ggaccatttc
     1801 tccggcgtcg ccgcatccta tgccgagtac cggcccaggt atcccaccga gctggtggac
     1861 ctgctggccg atctcgcgcc gcaccgcgac gtggcctggg atgcggggtg cggctccgga
     1921 cagctgtcgg tggccctggc ggagcgcttc gcccgggtta ttgccaccga cgcgagtgag
     1981 cagcagctgg cgcaggcgac cacccatcca cgcgtggagt accgccgggc gagtgcggag
     2041 gcgtccggga ttccctccgc caccgtggat ctcgcgacgg cggcacaggc ggtgcactgg
     2101 ttcgaccggc cggcctacta tcaagaggtg cgccgggtgt gccggcccgg cgccctgatt
     2161 gcgctcgtga cctacaatct gttccggatc acccccgagc tggaccggat catcgatcac
     2221 ttctactggc aaacgatctg gcagtactgg ccgccggaac ggcggctcgt cgaggccggc
     2281 taccgcacgc tcgactttcc gttcgccgag ctgcccgcgc cgccgctcga gatgcaggcg
     2341 gagtggaccc tgccgcacgt gctgggcttc gtgagtacct ggtcggcgct gcggggtctc
     2401 gaacgcgatc ggggcggccg tgtggtcgag gagttcgccc gcgaattgtc aggcgcgtgg
     2461 ggagctgccc aaacgccgcg cccggtgcgg tgggagctcg cggtccggct gggccgcgtt
     2521 tcctgacgaa gctacgggaa cctttcggcc gcctcagtcg attctgaagt gacatcagaa
     2581 tcggagtgag acggtgcaga aggtcgtgac gtgggctgtg caggcgggca tcgcggccgc
     2641 cctggcgatg ggcgcaacgc tcgccagggg gcgtgggacc actttgctgc tcgccggcat
     2701 ggcgggcggg gcggcgatcg ccagcgtact cggtttgtgg atgcggccgc cggaggcgct
     2761 cgcgcgccct cagcgggacg gcaccacgtc gatctccacg ctcgagggat agcgcgcggt
     2821 ccggtacacc cggtgaatct gcgcccggta atcctccgcc cgggcggtga agatgttcgg
     2881 cacgaaggtc tgcggattcc ggtcgtagag cggaaaccag gtgctctgca cctgcaccat
     2941 gatccggtgt cccttgcgga aggtgtagga ctgctggtgc aggtccacgg tgaacggcgt
     3001 gaccgcgctg ggagggatcc tcgtggggtg atcccacccc gcccggtagc gtccccgcat
     3061 gatctctccc gtcaccatca gctcgtaccc gcccatggtg ggccggtcgg ggacggtgtc
     3121 gggatacacg tcgatcagct tcaccaccca gtcggcgtcc gagcccgtgg tcgaagcgaa
     3181 caggcgggcg acgacgtttc ccgccacggt gacatccgct ccgagcgggg cggtttgcca
     3241 ggtgagcacg tcggtccggc cgtccacgaa acgctggtcc tcggtttccc agcgccgcca
     3301 gcgcgacgtg ggcgagtagg tgcgctccac cggacgcggg cggtagggca cgggacgagc
     3361 cgggtccgac acgtactgat cgaagccggc ggcggcggtg ggcggtctga aggagagccc
     3421 gccgtccgcc tggaagtaca ggcgccggtg cgcggccccg gcggggggcc agcggtcgaa
     3481 cgtccgccat tcgttcgtgc cggcgtcgaa cacggtcgcc tcgtcgaact ttccgtcgcc
     3541 ctggcctttc agccaatagg cgaaccagcg cgattgcagc gcgcggaagt ccgcccccgt
     3601 ggaccggtcg aagcggatgt tgcccagcgc gattccggaa tccgcgaacc actgtccgtg
     3661 gtaccacggt cccatcacga gaaaattctt tgcggcgttg tcgctgcgct cgagggccgc
     3721 gtaggaggcg agcgggccgt attcgtcctc ctggtcccac cagccgccca ccgtgagcgt
     3781 gggtacggtc gtgtgggtga aataccgggg cagcgaacgc gcctgccacg cggaatcgta
     3841 tgccggatgg gccacgaagc gtcgccaggt gggccagcga tcggcgccga ctgcggccgc
     3901 gagcgcgccc agcgtgggga acgaccgata ccattcgtag gtatcgtagc gcgccggcga
     3961 gggggtgacg ctcagatcgg gcgaggcctc catcatccag gcgtactcga ggccgtatgt
     4021 gaggcggaag gcgccctggt ggaagaagtc gtcgcccatc caggtgtcgc ccatcggggc
     4081 ctggggactg atcgccttga gcgccggatg cgcgccgatg ccgccgacgg cggtcagcca
     4141 gccgggatag gagatgccga agatcccgac ccggccgttg ttggacggca cgtgcttcac
     4201 gagccagtcg atggtgtcgt aggtatcggt gctctcgtcg gtgccggcgt gtccggagcg
     4261 cggcgggcgg ttcatgtcga acgccccctc ggacttgaag cggccgcgga tgtcctggaa
     4321 cacgaggatg tagccgtcga ggccaagcat tgcggcggcc tgcgggacgc cggccgccgg
     4381 gccggccgcg ccgtagggcg tgcgctgcat gagaatcggg tgcggcaggg tgtcgcgcgg
     4441 ggcgacgatg atcgtgttca gccgcaggcc gtcccgcatc gggatcatcg tgtcggtcgt
     4501 gacgaacgcc gagtccgggg cgggttgctg cgcctgcgcg acgg
//
LOCUS       DBNGZW010000561         4792 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_721344, whole genome shotgun sequence.
ACCESSION   DBNGZW010000561 DBNGZW010000000
VERSION     DBNGZW010000561.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4792)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4792)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4792
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_721344"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..1469
                     /gene="trmFO"
                     /locus_tag="AC16JC_13310"
     CDS             150..1469
                     /gene="trmFO"
                     /locus_tag="AC16JC_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007288448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HIR5165722.1"
                     /translation="MRATVVGGGLAGSEAAWALAERGIDVTLHEMRPVRATPAHQTDR
                     LAELVCSNSFKSVEVTNAHGLLKAEMRRLGSVILQAADAARVPGGAALAVDRVAFSRA
                     VHDRLSAHPRITLVRGEVTELPSPAVIATGPLTSEQLSAAIAQRLGAGTLAFFDAIAP
                     IVADDSLDHRALFRASRYGKGDGDDYLNAPLDEPAYEAFVTALLGADCYEPSHEFDRT
                     PYFEGCLPVEEMARRGRDVLRFGPMKPVGLTDPRTGRLPYAVVQLRREDRAGQMWNLV
                     GFQTRLRTGEQQRVLRSLPGLGSAEFLRFGSVHRNSYVNAPATLTPHLSARDDAKLLF
                     AGQLTGVEGYTESSATGLLAGLNLARMLYGEAPLLPPPTTMLGALYRYLREADPARFQ
                     PMNANWGLLEPLDRPVRDKARKRQLLAERALAGIETFAASFEGLVPA"
     gene            1466..2404
                     /locus_tag="AC16JC_13315"
     CDS             1466..2404
                     /locus_tag="AC16JC_13315"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR5165723.1"
                     /translation="MSAPPRPELAEFTAFLAKERNDSPHTVKAYARDVAGFAEFCDRY
                     YGGGGRWSWSGVDRLAIRGWLGALEERRLARRSAARALSALRTFYRFLAITHGTDVNP
                     ARAARTPKLERRLPTYLDRAETEQLFAEAERRAAADGGFRAARDLAMLELFYATGMRL
                     SELAGLSEPDLDIVSDQVKVRGKGRKERIVPLGAHAARALRAYFWERDRLLASRAPTA
                     GGRRAVFLNARGGRLSPRGVQLALRGLLDTLPRGRDLHVHSLRHSFASHLLDAGADLR
                     AVQELLGHASLSTTQVYTHTSVERLKQVYHRAHPRA"
     gene            2431..2970
                     /gene="hslV"
                     /locus_tag="AC16JC_13320"
     CDS             2431..2970
                     /gene="hslV"
                     /locus_tag="AC16JC_13320"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018955087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HIR5165724.1"
                     /translation="MSLPTFHATTVLCVRKDGQVAMGGDGQITVGDTVMKAHATKVRA
                     LKGGKILSGFAGAAADAFTLFEKFEEKLERYPGNLARAAVELAKDWRSDRVLRRLEAL
                     LAVADKEHGYVLSGTGELIEPDDGILAIGSGGSYALAAARALLAASALPPRDVVQQAL
                     TIAAEICIYTNANITILEL"
     gene            3061..4509
                     /gene="hslU"
                     /locus_tag="AC16JC_13325"
     CDS             3061..4509
                     /gene="hslU"
                     /locus_tag="AC16JC_13325"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009376 - HslUV protease complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HIR5165725.1"
                     /translation="MPWLEELPPKRIVAELDRYIVGQDEAKKSVAIAIRNRWRRSQAP
                     EDIRDEILPNNIIMIGPTGVGKTEIARRIARLAGAPFIKVEASKFTEVGYVGRDVESM
                     VRELVDMSINMVRTEREDEVYPQAEERAEERLLDILLPPPPSTPTPRSSLPGEEATGG
                     GGRPLFVVSSKGDVTPKTPEADLEAQERRRRTREKLRQQLKEGKLEERDVEIEVQQQS
                     YPMLEWMQPPPGMEGQDVNFSEMLQEMLPKKKKRRTVHLHEARRILVDEELKKLVDMD
                     DVVNEALDRVENHGIIFIDEIDKIAGERNTAGPDVSREGVQRDLLPIVEGSTVQTRYG
                     YVRTDHVLFIAAGAFHVSKPSDLIPELQGRFPIRVELSPLGEADFVRIMTEPENALTK
                     QYAALCQVEGATLTFTPDGIQEIARIAAKVNERMENIGARRLHTVMSTLLETVLFELP
                     DYPTKQIVFDAPAVRTRLEKIVDDEDLRRYVL"
     gene            4506..>4792
                     /locus_tag="AC16JC_13330"
     CDS             4506..>4792
                     /locus_tag="AC16JC_13330"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR5165726.1"
                     /translation="MTGARRPARFTREFPVAPADIDELGHVNNVVYLRWVQEIAVAHW
                     RAAAPAALQAEVVWVVLRHELDYKRPARAGETVVGRTWVGEATGSRFERFT"
BASE COUNT          733 a         1676 c         1725 g          658 t
ORIGIN      
        1 cgcccgtacg cgacggagat ctgcccgagc tgcgggttcg ccggcatgac gaagaaggag
       61 accaaggacg ggagagtcac gcgcaagtgc ctgaagtgcg ggttcgagga cgccggagcg
      121 gagatcgccc caccggagcc ggacgcggcc tgagggcgac cgtcgtgggc ggcggcctcg
      181 ccggcagtga ggccgcctgg gcgctggccg agcgcggcat cgacgtgacg ctccacgaga
      241 tgcgtccggt ccgggccacg ccggcccacc agaccgaccg cctcgccgag ctggtctgca
      301 gcaactcgtt caaatcggtc gaggtcacca acgcccacgg cctgctcaag gccgaaatgc
      361 gccggctggg gagcgtgatc ctccaggcgg ccgacgcggc ccgggtcccg gggggtgcgg
      421 cgctggcggt ggaccgggtc gcgttctccc gtgccgtgca cgaccgcctc agcgcccatc
      481 cgcgcatcac cctcgtgcga ggggaggtca ccgagctgcc gtctcccgcc gtcatcgcga
      541 ccgggccgct cacctccgaa cagctgagcg ccgccatcgc gcagcgcctc ggcgccggga
      601 cactggcgtt cttcgacgcg atcgcaccca tcgtcgccga cgactccctc gatcaccgcg
      661 cgctgttccg ggcgtcccgc tacggcaagg gcgacgggga cgactacctc aacgccccac
      721 tggacgagcc ggcgtacgaa gccttcgtca ccgccctgtt gggcgcggac tgctacgagc
      781 cgtcgcacga gttcgaccgc acgccctact tcgagggctg cctgccggtg gaggagatgg
      841 cgcggcgcgg ccgcgacgtg ctgcgcttcg gacccatgaa gcccgtcggg ctgaccgacc
      901 cgcgcaccgg gcggctgccg tacgccgtgg tgcagctgcg gcgggaggat cgcgccgggc
      961 agatgtggaa cctcgtcggg ttccagaccc ggttgcgcac cggcgaacag cagcgcgtgc
     1021 tgcggtctct ccccgggctg ggcagcgccg agttcctgcg tttcggcagc gtgcaccgca
     1081 actcctacgt caacgccccg gcgaccctga cgccgcatct ctcggcgcgc gacgacgcga
     1141 agctgctgtt cgcgggccag ctcacgggcg tggaagggta caccgagtcg tcggccaccg
     1201 ggctgctcgc ggggttgaac ctcgcccgga tgctgtacgg cgaggcgccg ctcctgccgc
     1261 cgccgaccac gatgctgggc gcgctgtacc gctatctgcg ggaggccgac ccggcgcggt
     1321 tccagccgat gaacgcgaac tggggcctgc tcgagccact cgatcgaccg gtgcgcgaca
     1381 aggcgcgcaa gcgccagctg ctcgcggagc gggcgctcgc cggcatcgag acgttcgcgg
     1441 cgtcgttcga aggattggtg ccggcgtgag cgctcccccc aggcccgagc tcgccgagtt
     1501 cacggccttc ctggccaagg agcgcaacga ttcgccgcat accgtgaagg cgtacgcgcg
     1561 cgacgtcgcg gggttcgcgg agttctgcga ccgctactac ggcgggggcg gtcgctggag
     1621 ctggagcggc gtggaccgcc tggcgatccg cggctggctg ggggcgctgg aagagcgccg
     1681 gctggcccgc cgctcggcgg cgcgggcctt gtcggcgctg cggaccttct accggttcct
     1741 cgccatcacg cacggcacgg atgtgaaccc ggcgcgggcc gcgcgcacgc cgaagctcga
     1801 gcgccggttg ccgacgtacc tggaccgcgc ggaaaccgag cagctgttcg cggaagccga
     1861 gcgtcgcgcg gcggcggacg gaggatttcg ggccgcgcgc gacctcgcca tgctcgagct
     1921 gttctacgcg accggcatgc ggctgagcga gctggccggg ctgtcggagc cggatctgga
     1981 tatcgtgtcc gaccaggtga aggtgcgcgg caagggccgc aaggagcgga tcgttcccct
     2041 gggggcccac gccgcccgcg ccctgcgcgc ctatttctgg gagcgcgacc ggctgctcgc
     2101 gtcgcgggcg cccaccgcgg gcgggcggcg ggcggtgttc ctgaacgcgc gcggtgggcg
     2161 gctcagcccg cggggcgtgc aactcgcgct gcgcgggctg ctcgacaccc tgccgcgcgg
     2221 gcgcgatctg cacgtgcact cgctgcgcca ttccttcgcg agccacctgc tggacgcggg
     2281 cgccgacctg cgggcggtgc aggagttgct cgggcacgcc tcgctcagca cgacgcaggt
     2341 gtatactcat acgtcggtcg aacggttgaa gcaggtgtat caccgggccc atccgcgggc
     2401 ctgacggaac gggaaagggc gaggtccccg gtgtcgctgc ctacgtttca cgccacgacg
     2461 gtgttgtgcg tccgcaagga cggccaggtc gcgatgggcg gcgacggcca gatcacggtc
     2521 ggcgacaccg tcatgaaggc gcacgccacg aaggtgcgcg ccctcaaggg cggcaagatc
     2581 ctctcggggt tcgccggcgc ggcggccgac gccttcacgc tgttcgagaa gttcgaggag
     2641 aagctggagc gctatcccgg caatctggcg cgcgccgcgg tcgagctcgc caaggactgg
     2701 cgctcggacc gcgtgctgcg gcgcctcgag gccctgctcg ccgtggccga caaggagcac
     2761 ggctacgtgc tctccggcac cggggagctg atcgagcccg acgacggcat cctcgcgatc
     2821 ggctcggggg gcagctatgc cctcgccgcc gcgcgcgcat tgctcgccgc ctccgccctg
     2881 ccgccgcgcg acgtggtgca gcaggcgctc acgatcgccg cggagatctg catctacacg
     2941 aacgccaaca tcacgattct cgagctctag ccgatggccg atccgaccgc tactccgccc
     3001 ccgcccccgc cgccctccga gcaggtcccc ccggccgctg cgccgccggc ggaggaggcg
     3061 ctgccctggc tcgaggagct gccgcccaag cggatcgtgg cggagctgga tcgctacatc
     3121 gtggggcagg acgaagcgaa gaagtcggtc gcgatcgcca ttcgcaaccg ctggcgccgc
     3181 tcccaggcgc ccgaggacat ccgggacgag atcctgccca acaacatcat catgatcggc
     3241 cccacgggtg tggggaagac cgagatcgcg cggcgcatcg cgcggctggc gggcgcgccg
     3301 ttcatcaagg tagaggcgtc gaagttcacc gaggtggggt acgtcgggcg cgacgtcgaa
     3361 tcgatggtgc gcgagctggt ggacatgtcc atcaacatgg tgcgcaccga acgcgaggac
     3421 gaggtctacc cgcaggccga ggagcgcgcc gaggagcgcc tgctcgacat cctgctccca
     3481 cctccgccca gcactcccac tccccgctct tcactcccgg gggaggaggc gacggggggt
     3541 ggcgggcgtc ccttgttcgt cgtgtcgtcc aagggggacg tgacgccgaa gacgcccgag
     3601 gccgacctcg aagcgcagga gcggcgccgc cgcacgcgcg agaagctgcg ccagcagctt
     3661 aaggaaggaa agctcgagga gcgggacgtc gagatcgagg tacagcagca gtcctacccg
     3721 atgctggagt ggatgcaacc gccgcccggg atggaggggc aggacgtcaa cttctccgag
     3781 atgctgcagg agatgctgcc gaagaagaag aagcggcgca ccgtccacct gcacgaagcg
     3841 cggcgcattc tggtcgatga ggagctgaag aagctggtgg acatggacga cgtggtgaac
     3901 gaggcgctcg accgcgtcga gaaccacggg atcatcttca tcgacgagat cgacaagatc
     3961 gcgggtgaac gcaacacggc cggacccgac gtatcgcgcg agggcgtgca gcgcgacctg
     4021 ctgccgatcg tcgaaggctc gacggtgcag acccgctacg gctacgtgcg caccgatcac
     4081 gttctgttca tcgccgccgg ggcgttccac gtctccaagc cgtcggatct gatcccggag
     4141 ctgcagggac ggttcccgat ccgcgtggag ctgtcgccgc tgggcgaagc ggacttcgtc
     4201 cgcatcatga ccgagcccga gaacgccctg accaagcagt acgcggccct gtgccaggtg
     4261 gaaggggcca ccctcacctt cacgcccgac ggcatccagg agatcgcccg catcgcggcc
     4321 aaggtgaacg agcggatgga gaacatcggc gcgcgccggc tgcacaccgt catgagcacc
     4381 ctgctcgaaa ccgtgctgtt cgagctgccc gactatccca ccaagcagat cgtgttcgac
     4441 gctccggccg tgcggacccg cctcgagaag atcgtcgacg acgaggactt gcggcgctac
     4501 gtcctgtgac cggggcgcgg cggccggcgc gcttcacgcg cgagttcccc gtcgcccccg
     4561 ccgacatcga cgagctgggt cacgtgaaca acgtcgtcta cctgcgctgg gtgcaggaga
     4621 tcgccgtggc ccactggcgt gccgccgcgc cggccgccct ccaggccgag gtggtgtggg
     4681 tggtgttgcg ccacgagctg gactacaagc gccccgcgcg cgccggcgag accgtggtcg
     4741 gccgcacctg ggtgggcgag gcgaccggct cccgcttcga gcggttcacg ga
//
LOCUS       DBNGZW010000562         2513 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_189279, whole genome shotgun sequence.
ACCESSION   DBNGZW010000562 DBNGZW010000000
VERSION     DBNGZW010000562.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2513)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2513)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2513
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_189279"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..413
                     /locus_tag="AC16JC_13335"
     CDS             <1..413
                     /locus_tag="AC16JC_13335"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5165727.1"
                     /translation="AADVIQESLVRAFRSLRRCQDRSNFKGWLFRIVTNQCKTHITRR
                     RRRSLESLDAATEIPAVENPVADAEAADLQRRVRQALQDLPREQREALVLKYIEGLSL
                     PEMAEALSVSVPALKMRLLRARRELLAKVQGVLV"
     gene            410..970
                     /locus_tag="AC16JC_13340"
     CDS             410..970
                     /locus_tag="AC16JC_13340"
                     /inference="COORDINATES: protein motif:HMM:NF027875.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HIR5165728.1"
                     /translation="MTAEDHEEREARDQEAERLMGTLDRSPVHLPALRQMVMAQIRRE
                     QSSPWIRAWRWLAAPQLSPLTAGAALVAGVALLLWLRPFSHTVTTPPESRTVPVRLVF
                     LAPQAATVAVTGDFARWDPHGIPLTRPQGNGAWSVELRLPPGLHHYVFIVDGTEWRPD
                     PNAASQVDDGFGQKNSVLLVPGPRIT"
     gene            967..1638
                     /locus_tag="AC16JC_13345"
     CDS             967..1638
                     /locus_tag="AC16JC_13345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165729.1"
                     /translation="MTTRRLLHVMAVGCLATVPLDAQGRDPIEQRLDPQTFAAVRAIF
                     DSARRDSVPAQALEDKALEGVAKNVPPARIVAAVRQLAAELHDARALLRTAAPNATLG
                     DGEIIAAADAQRRGVPPVELSSLRGHVSSRASLVVAFTVLGDLVQRGVPAGQARDVVE
                     QLIEAGVSAQQLADVPARMDVGLRVGAPPLDALRSALPVPLRPVRPPPGAIPPKPNPS
                     GHGYF"
     gene            1640..>2513
                     /locus_tag="AC16JC_13350"
     CDS             1640..>2513
                     /locus_tag="AC16JC_13350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165730.1"
                     /translation="MKPQSISSALLLAAGLAVCASPAGAQRWQVDLAGNSVGYDTAGR
                     ITSASVAPLIEWSQRSVYATLSGALAAFEAGQWTSQGHGDVSLLFVPARSLRLVRGEV
                     VGSADGSVHSSGYRTAGTRGELRLHVAGAAAGLWIGATAGTGWTSSSTGITTAIGPTA
                     GAWGRHGSWTATAVWTPFRLAGAWYQQMEGRVTTSLGPVDATAYGGWRGAPGSSGLPD
                     GGWGGGTLTIWIARQAAVLLSGGSYASDLLQALPRGRYLSAGLRLSLGRPSLWAGTTA
                     RPLYTRAAGEAELRF"
BASE COUNT          361 a          874 c          897 g          381 t
ORIGIN      
        1 aagcagcgga cgtcatccag gagagcctgg tccgggcgtt tcgctcgctg cgccgctgcc
       61 aggaccggtc gaacttcaag gggtggctgt tccggatcgt cactaaccag tgcaagacgc
      121 acatcacccg aaggcgacgg cgctcgttgg aatccctgga tgcagcaacc gaaattccgg
      181 ccgtcgagaa cccggtggcg gacgccgagg cagccgatct tcagcggcgg gtacggcagg
      241 cattgcagga tcttccgaga gagcagcgcg aagccctggt cctcaagtac atcgagggat
      301 tgagccttcc ggagatggcc gaggcgctct cggtcagcgt gccggcgctc aagatgcggc
      361 tgttgcgcgc gcgccgggag ctgttggcca aggtgcaggg ggtgctcgta tgacagcaga
      421 ggatcacgag gagcgggaag cccgagacca ggaggccgag cggctgatgg ggaccctcga
      481 ccgctcgccc gtccatctgc ccgcgctgcg gcagatggtg atggcacaga tccgtcgcga
      541 gcagtcctca ccgtggatcc gcgcgtggcg gtggctggcg gcaccgcagc tcagtcccct
      601 caccgcggga gccgccctgg tcgccggcgt ggcgctgctg ctgtggctgc gccccttctc
      661 tcacacggtg acgacgccac cggaatcgcg taccgtgccc gttcgcctcg tcttcctggc
      721 cccgcaggcg gctacggtgg cggtcacggg cgatttcgcg cgctgggacc cccacggaat
      781 cccgctcacc agaccacagg gaaacggcgc ctggagcgtg gagctgcggc tgccacccgg
      841 actccaccac tatgtgttca tcgtggacgg gacggaatgg cggcccgatc ccaacgcggc
      901 gtcgcaggtg gacgacgggt tcggacagaa gaacagcgta ctgctcgtgc cggggccgag
      961 gatcacgtga cgacacgccg attgctgcac gtcatggccg tcggttgtct ggccacggtt
     1021 cccctcgacg ctcagggtcg cgacccgatc gagcagcgac tcgacccaca gaccttcgcg
     1081 gcggtccgcg ccattttcga cagcgcccgc cgcgattcgg ttccggccca ggcactcgaa
     1141 gacaaggcgc tcgaaggcgt ggcgaagaac gtcccgccgg ctcgcatcgt ggccgcggtt
     1201 cgccagctcg cggctgagct gcacgatgcg cgagcgctgc tgcgaacggc ggcccccaac
     1261 gccaccctcg gcgacggcga gatcatcgcg gctgccgacg cgcagcgccg cggcgttccc
     1321 ccggtcgagc tctcgtcgct ccggggtcat gtgtcgtcca gggcatcact ggtcgtggca
     1381 ttcacggtgc tcggggatct ggtacagcgc ggcgttcccg cgggccaggc ccgcgacgtg
     1441 gtcgaacagc tcatcgaagc gggcgtctcc gcgcaacagc tggccgacgt tccggcacgc
     1501 atggacgtcg ggctccgggt cggggcgccg ccgctcgacg cgttgcgcag cgcgctccct
     1561 gttccgctgc gtcccgtcag accgccaccg ggcgccatac cgcccaagcc gaatcccagc
     1621 gggcacggct acttctgaca tgaagccaca atcgatctct tcggccctcc tcctcgcggc
     1681 gggccttgcc gtctgtgcgt cgccggcggg tgcgcagcgc tggcaggtgg acctggccgg
     1741 taacagcgtg gggtacgaca cggccggccg gatcacctcg gcatccgtcg ccccgctcat
     1801 cgaatggagc cagcggtccg tctacgcgac gctgagcggc gcactcgcgg ctttcgaggc
     1861 gggccagtgg acgtcccagg ggcacggtga cgtgtcgctg ctgttcgtcc cggcacgctc
     1921 gctgcggctg gtacgcgggg aggtcgtggg ctcggccgac ggtagcgtgc actcgagcgg
     1981 ctaccgcacg gccggcaccc gtggcgagct gcggctgcac gtcgcgggcg ccgcggccgg
     2041 actctggatc ggcgccaccg ccggcaccgg atggacctca tccagcactg gtatcacgac
     2101 ggccatcgga cccacggcag gcgcgtgggg ccgccatggc agctggaccg cgacggcggt
     2161 gtggacgccg tttcggcttg ccggagcctg gtaccaacaa atggaaggcc gtgtgacgac
     2221 ctcgctcgga cccgtggacg ccacggccta cggaggctgg cgcggggcac cgggaagctc
     2281 cgggctgccc gacggcggct ggggcggcgg tacgctcacc atctggatcg cgcgtcaagc
     2341 cgccgtcttg ttgagcggcg ggagttacgc ctccgatctt ctgcaggcgc tcccgcgggg
     2401 gcggtatctg tccgcgggac tccggctgtc gctgggccgt ccctccctct gggcgggcac
     2461 gaccgcccgc ccgctgtaca cgcgcgcggc gggtgaggcg gagctgcggt tca
//
LOCUS       DBNGZW010000563         9131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_189334, whole genome shotgun sequence.
ACCESSION   DBNGZW010000563 DBNGZW010000000
VERSION     DBNGZW010000563.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 9131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9131
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_189334"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="AC16JC_13355"
     CDS             complement(<1..356)
                     /locus_tag="AC16JC_13355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR5165731.1"
                     /translation="MPLFEYTARNLKGDLVKDQVDLPSKDDVIAHLRKNRLVVVQVRQ
                     APASVSLGGMFKGGIKTRDVVIFTRQFATMINAGLPLVQALDILAQQTENKVLAGVTR
                     QVVYDVESGQTLADALR"
     gene            complement(381..1514)
                     /locus_tag="AC16JC_13360"
     CDS             complement(381..1514)
                     /locus_tag="AC16JC_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002629218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR5165732.1"
                     /translation="MPVNLRALLEEMIEKDASDLHIVAGERPKLRIDGDITSASTEDV
                     LTPKDTLSLAYSVLTENQKKRFETENELDFSFGIQNLARFRGNCFKQRGCVSLVIRQI
                     PFNVRTFEDLGLPPIVAKLAERPRGLVLVTGPTGSGKSTTLAAMIDKINKELKGHIIT
                     VEDPIEFIHRHQQCLVNQREVGTDTNSFAAALKYALRQDPDVVLIGEMRDLETTQSAL
                     TIAETGHLAFATLHTNSAAETINRIIDIFPAHQQSQIRAQLAFVLEGVITQTLLQKAK
                     GRGRVMAAEIMVCTPAIRALIRDDKVHQIYSAMQAGKKHGMQTLNDALYQLYMQRDVI
                     KEECLRVSSDPTEFLRMIGETPVEEGSGGPPAAAGASRVAARR"
     gene            complement(1528..3240)
                     /gene="pilB"
                     /locus_tag="AC16JC_13365"
     CDS             complement(1528..3240)
                     /gene="pilB"
                     /locus_tag="AC16JC_13365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011939532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HIR5165733.1"
                     /translation="MRTSTAALSIDKLGDILVRDGLITADQLKRALAEQRGSGMRLGY
                     TLVKLGFIEETEITKMLARQYRMPAVDLSRFEVDPKILKLISPDIAAKYTVLPLKREG
                     RTLTIAIADPNNVAAIEDIKFITRCDIFPVIAGEYTLRNAIDRYYQQSDAQLQSLLKS
                     VEDADTGDLEVLEEQMDEEVKASELAEDAPVVKLINGLLTDAVKRGASDIHIEPFEHE
                     MRVRYRVDGALQEVMRPPVKMRAALTSRVKIMAQLNIAERRVPQDGRIKLKMGARVID
                     FRVSTLPVLFGEKIVLRILDKGNLTFDLTTFGFEPKAQADLMKAILNPYGMVLVTGPT
                     GSGKTTTLYSALSKINTIEVNIMTAEDPVEYNLMGINQVLVRNEVGMTFAAALRAFLR
                     QDPNIIMVGEIRDLETGSIAIKAALTGHLVLSTLHTNDAPSTVTRMIDMGIEPFNVAS
                     AVNLIVAQRLVRRICAECKQEHEYSDEEMHAFGITRDQGPFFKGAGCDTCNGTGYRGR
                     QGLYEVMALSSNLRRQILKGASTEELREVAVSEGMLTLRMDGMVKVKRGVTTLEEVVK
                     ETAA"
     gene            complement(3286..3687)
                     /locus_tag="AC16JC_13370"
     CDS             complement(3286..3687)
                     /locus_tag="AC16JC_13370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015235908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HIR5165734.1"
                     /translation="MDDKRAALMLAGLALTGAAVRVVLSPSPDRAPGDVRLVSSDTLP
                     SSALRETARRAAQLARPLAPGERVDVDRADVTELTRLPRVGPALAQRIVAWRERHGPF
                     GTLTRLDSVSGIGPKLLDAIRPYVAFSGALP"
     gene            complement(3941..4876)
                     /locus_tag="AC16JC_13375"
     CDS             complement(3941..4876)
                     /locus_tag="AC16JC_13375"
                     /inference="COORDINATES: protein motif:HMM:NF016289.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR5165735.1"
                     /translation="MSGAARVRLAAALAGALLAACGARSKALVREFDGARAFTYLERQ
                     VRFGPRIPGTPGHDSTGDWILARLRERADTVIVQDIPHRTAGGQTIHLRNFLARFRPA
                     GAERVLYLAHWDTRPRADQSANLGAQRLPVPGANDGASGVAVLLGVADALKARPPALG
                     VDLLFVDGEDYGDFADTTAVLIGSRWFAAHQPDGYPPLFAVLFDMVGDKDLHLPYEQN
                     SQGFAPEVVDRVWSVADSIGYGSVFARQPGPSLIDDHVALQHANIHAIDVVDFSYGPS
                     NGYWHTTEDTVDKVSAQSLQIVGDVAVAVVRTMGR"
     gene            complement(4873..9096)
                     /gene="sov"
                     /locus_tag="AC16JC_13380"
                     /gene_synonym="sprA"
     CDS             complement(4873..9096)
                     /gene="sov"
                     /locus_tag="AC16JC_13380"
                     /gene_synonym="sprA"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071806 - protein transmembrane transport
                     [Evidence IEA];
                     GO_process: GO:0160303 - protein secretion by the type IX
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS outer membrane translocon Sov/SprA"
                     /protein_id="HIR5165736.1"
                     /translation="MAATYDLGQLGQVHLTGLFQKEQTIFTRPQLGFEPASGFIGGVS
                     ANLRFQPEWITRAVHLIPGARSDAPSFLTVSGEVALSRPRPNPLGQAYLEEFEAQAGR
                     FISLDENAWHWGSVPSSSRGATAFDIPATGFDTLDAVPLTWQSLPLADSSGRVAQFLA
                     TQIDPTIVLTGQVQSAEPVLWLMLKPDTMMGLADSRTGIPNWRRPGSGNNARLTTRWR
                     SITQTLSTSGIDLSRVEYLEFWVWEDELRTAKTNRTAVLFDFGSVFEDALAFVPETLR
                     VSPAGDTTYSGVRIAGVGRLDTERDPVTHSWSASVNDEGILSDRVENIVDSATGQVIP
                     ELPLCSASVNGQIVSYLFGDPRSRCGRHNGAVDTEDQDGDAQLDVAVGAKTQEDFVRF
                     VFPVGDDRYYVRDGGMVQTSRGASGWRLFRIPFRTDTLQVGSPNLRQVQTLRITVLAP
                     QTAAPGDPDPQVYFALSRVRLVGATWLKRAETPLAGVGGDRATGLGEVVASVVSTENH
                     DLGYTPPPGVGNEAARQDQSFQVGTTQINEQSLRLLARGLQLGQRAEAFTRFGAEGDK
                     NFLKYRQLRVWARGRGPGWEDGDLEFFLKVGKDADNFYFYHTPARTASWDPEVVIALD
                     RWVALRTHIQQAWLAGDPPHVYPGCPAPPLVPDDTAYVMCDGAYIAHVKVPGGAPPNL
                     AAVQELAAGIWRVGAATFIDQAEVWVDDIRLSDVVQNAGTAAALNVALTAANLADVSL
                     DVSRRDGNFRQLGEDPSYQTNQAYSLVTTLHLERLLPPQWGLAAPLQMRYAAIANEPV
                     FLAGTDLRGAALAGLRTPLTRARSFAFSLRRVRRASAGPARWLADPLSLTAAYTSGDD
                     RSSLSAATARNYAVSVDYGLQPRGATIPAAPRFLVALVRKLPPFIGKSGFADGLERAR
                     LRLSPTSIRLRSTLSGGDASQTVFRIPVADTGDRHLLPARSLTRVWRNSASLDLLPLS
                     NVQVRGDLASTRDLRDYGDSSSIARVARLARKSLLGVDVGMETQRTIGTFYGMTPRFL
                     PWLSPRVSLTTGFSLTRDANGRAPVRTAGDSGAFHLPTAFSNSQRVELGTQLDLGRLG
                     RGIFGDSSPIARALGALTSLDLGFNRDRTSIYNSLAAAPSLTYQLGLVGLGALRSQRD
                     IPATGATDNNSRRAAGTVSLPLGIRVTGLYQRTGGTTWLLRFDGQVPTTTSSREWPSG
                     NVSWTFSPPRTTIGRVLSGLTAQLSVRHRQTENRQSTLSGVSGGGGTESVTRMDEHTL
                     APGVTASWAHGVLTSFDGTRLRTEQVTAGNTFRNMRTQQNASLAFAWRPPAALVRLKT
                     DIRTTARYSYTQNTTCLISSNQGPCRPYVDSRQTNAQLTLDTSFPPSLSAGLQMAYVL
                     DDERQINRRIAQLVLTAFLQLNTSVGQVR"
BASE COUNT         1412 a         3070 c         3157 g         1492 t
ORIGIN      
        1 cgcagggcgt cggccagggt ctggccactt tccacgtcgt agacgacctg gcgggtgacg
       61 ccggcgagca ccttgttctc ggtctgttgc gccaggatgt ccagcgcctg gacgaggggc
      121 agtccggcgt tgatcatcgt ggcgaactgg cgggtgaaga tgacgacgtc ccgggtcttt
      181 atcccgcctt tgaacatgcc gccgagcgag accgacgcgg gggcctgccg cacctgtacc
      241 accacgaggc ggttcttgcg caggtgggcg atgacgtcgt ccttgctcgg caggtcgacc
      301 tgatccttga ccaggtcgcc cttgagattg cgcgcggtgt attcaaacag tggcatgact
      361 caccctcctg ccggtcgtgg tcagcgccgg gcggcgacgc gcgaagctcc cgcggccgcc
      421 ggcggtcccc cactgccctc ctcgaccggc gtctcgccga tcatccggag gaactcggtg
      481 gggtcggatg agacgcgcag gcattcctcc ttgatcacgt cgcgctgcat gtacagctga
      541 tacagggcgt cgttgagcgt ctgcatgccg tgcttcttcc ccgcctgcat cgcggagtag
      601 atctggtgca ccttgtcgtc gcggatcagc gcgcggatcg ccggcgtgca caccatgatc
      661 tccgccgcca tgacccggcc ccggcccttc gccttctgca gcagcgtctg ggtgatcaca
      721 ccctcgagca cgaacgccag ctgggcgcgg atctgggact gctgatgcgc cggaaagatg
      781 tcgatgatgc ggttgatcgt ctccgccgcc gagttggtgt gcagcgtggc gaacgccagg
      841 tgcccggttt cggcgatggt gagcgccgac tgtgtggtct cgaggtcgcg catctcgccg
      901 atcagcacga catcggggtc ctggcgcagc gcgtacttca gggccgccgc gaagctgttc
      961 gtgtcggtcc ccacctcgcg ctggttcacg agacactgct ggtggcggtg gatgaactcg
     1021 atcgggtcct ccaccgtgat gatgtgcccc ttcagctcct tgttgatctt gtcgatcatc
     1081 gcggccagcg tcgtgctctt ccccgagccg gtcgggccgg tgaccagcac caggccgcgc
     1141 ggccgctcgg ccagcttggc gacgatcggc ggcagcccca gatcctcgaa ggtgcggacg
     1201 ttgaagggga tctgccggat caccagcgac acgcagccgc gctgcttgaa gcagttgccg
     1261 cggaagcgcg ccaggttctg gatcccgaag gagaagtcga gctcgttctc cgtctcgaag
     1321 cgcttcttct ggttttcggt gagcaccgag taggcaagcg acagcgtgtc cttcggcgtg
     1381 aggacgtcct cggtggacgc gctcgtgatg tcgccgtcga tccgcagctt gggacgctcc
     1441 cccgccacga tgtggaggtc ggaggcgtcc ttctcgatca tctcctcgag caacgctcgg
     1501 aggttcaccg gcatcgggtc gcctccttca ggcggcggtt tccttcacga cttcctcgag
     1561 cgtggtcacg ccccgtttca ccttcaccat gccgtccatc cgcagcgtca gcatcccctc
     1621 cgacaccgcg acttcccgca actcctcggt cgacgccccc ttgagaatct ggcggcgcag
     1681 attggaggac agcgccatca cttcgtacag cccctgacga ccccggtacc ccgttccgtt
     1741 gcaggtgtcg cacccggcgc ccttgaagaa cgggccctgg tcgcgcgtga tgccgaaggc
     1801 gtgcatctct tcgtcggagt attcgtgctc ctgcttgcac tccgcgcaga tgcgccgcac
     1861 caggcgctgg gccacgatca ggttgaccgc gctcgccacg ttgaacggct cgattcccat
     1921 gtcgatcatg cgcgtgacgg tcgacggcgc atcgttggtg tgcagggtgg acagcacgag
     1981 gtgtccggtg agtgccgcct tgatggcgat cgagcccgtt tccaggtcgc ggatctcgcc
     2041 caccatgatg atgttcggat cctgccgcag gaacgcccgc agcgccgccg cgaacgtcat
     2101 ccccacctcg ttgcggacca gcacctgatt gatccccatc aggttgtact cgaccggatc
     2161 ctcggcggtc atgatgttca cttcgatcgt gttgatcttc gagagcgccg agtacagcgt
     2221 cgtcgtcttc cccgagcccg tcggccccgt gaccagcacc atgccgtagg gattgagaat
     2281 ggccttcatc aggtcggcct gggccttggg ctcgaacccg aacgtggtca gatcgaacgt
     2341 caggttcccc ttgtcgagga tccgcagcac gatcttctcg ccgaacagca ccggcagcgt
     2401 ggacacccgg aagtcgatca cgcgagcgcc catcttcagc ttgatgcgcc cgtcctgcgg
     2461 cacgcggcgc tcggcgatgt tgagctgcgc catgatcttc acgcgcgacg tcagcgccgc
     2521 ccgcatcttc accggcggcc gcatcacctc ctgcagcgcc ccgtccaccc gatagcgcac
     2581 ccgcatctcg tgctcgaagg gctcgatatg gatgtcggac gccccgcgct tcaccgcgtc
     2641 ggtgagcagg ccgttgatca gcttcaccac cggcgcgtcc tcggccagct cggaggcctt
     2701 gacctcctcg tccatctgct cttcgaggac ctccaggtcg cccgtgtccg cgtcctcgac
     2761 gctcttcagc aggctctgga gctgcgcgtc cgactgctgg tagtagcggt cgatcgcgtt
     2821 gcgcagcgtg tactccccgg cgatcaccgg gaagatgtcg cagcgcgtga tgaacttgat
     2881 gtcctcgatc gccgccacgt tgttcggatc ggcgattgcg atggtgaggg tgcgcccctc
     2941 gcgcttgagc ggcaggacgg tgtacttggc cgcgatgtcg ggcgagatca gcttgagaat
     3001 cttggggtcg acttcgaagc gcgacaggtc caccgccggc atgcggtact gccgcgccag
     3061 catcttggtg atctcggttt cctcgatgaa accgagcttg accagggtgt acccgagccg
     3121 catcccggag ccgcgttgct cggcgagggc ccgcttcagc tggtccgccg tgatcaaccc
     3181 gtcgcggacc aggatgtcgc ccagcttgtc gatggagagt gctgcggtgc tcgtacgcaa
     3241 ctcgttgaaa ttgaatgaac taaactagtt ggacggggtg gcctgtcaag gcagtgctcc
     3301 cgaaaacgcg acgtaagggc ggatcgcgtc gagcagtttg gggccgatcc ccgacacact
     3361 gtcaagtcgg gtgagcgtcc cgaacggccc gtgacgctcc cgccaggcga cgatgcgctg
     3421 cgccagcgcg ggaccgacgc gcggcagccg tgtgagttcc gtcacatcgg cgcggtccac
     3481 atccacccgc tcaccgggag ccagcgggcg cgcgagctgg gcggcgcggc gcgcggtctc
     3541 gcgcagcgcg gaggacggga gcgtgtcgga ggacacgaga cgcacgtcgc ccggcgcgcg
     3601 atcgggagaa ggggacagga caacacggac ggctgcgccc gtcagcgcga gaccggccag
     3661 catcagagcc gcgcgtttgt cgtccatcac cgcagactaa ccaacaggct ggcgatgggt
     3721 gggaccggaa gattgcgacc gggatcgatc ttacgcggag tgcaggcgtc agacggacgg
     3781 acagatgttc agacggacag acagtcactc agatgctcag gcgcatagcg gttatcagtc
     3841 acgagtcact cagaaaaggc ctctgctgag tcactgaagg ctgacaaccg ctaagcgcct
     3901 gagcatctga gcatctgtct gtccacctgt ggactgtagt tcatcgcccc atcgttctca
     3961 ccactgccac ggccacatct cccacaatct ggagcgactg cgcgctcacc ttgtccaccg
     4021 tgtcttctgt cgtgtgccag tacccgttgc tgggcccgta gctgaagtcc accacgtcga
     4081 tggcgtggat gttcgcgtgc tgcagcgcca catgatcgtc gatcagggac gggcccggct
     4141 gccgcgcgaa cacgctgccg tacccgatgg aatccgccac cgaccacacc cggtccacga
     4201 cctcgggggc gaagccttga gagttctgct cgtaggggag atggaggtcc ttgtccccca
     4261 ccatatcgaa caacacggcg aagaggggtg ggtacccatc cggctgatgg gcggcgaacc
     4321 agcgcgagcc gatcaggacg gcggtcgtat cggcaaagtc gccgtaatcc tctccgtcca
     4381 cgaacagcag atccactccc agggccgggg ggcgcgcctt gagggcatcg gcgacgccga
     4441 gcaacacggc cacgccggac gcgccgtcgt tcgccccggg gaccggcaga cgctgcgcgc
     4501 cgaggttggc gctctggtcg gcgcgcggcc gcgtgtccca gtgggccagg tagagcacgc
     4561 gctcggcgcc cgccgggcgg aatcgcgcca agaaattgcg cagatggatc gtctgcccgc
     4621 cggcggtgcg gtgggggatg tcctgtacga tcacggtatc ggcgcgctca cgcagccgcg
     4681 ccagaatcca gtccccggtc gagtcgtggc cgggggtgcc gggaatccgg ggcccgaagc
     4741 gtacctgccg ctccaggtag gtgaaggccc gcgcgccgtc gaattcgcgg acgagcgcct
     4801 tgctccgggc gccgcaggcc gccagcagcg cgcccgccag cgcggcggcg agacgcacac
     4861 gagcggcgcc gctcatcgga cctgccccac gctggtgttg agctgcagga acgccgtgag
     4921 gacgagctgc gcgatccggc gattgatctg ccgctcatcg tcgagaacgt aggccatctg
     4981 taaccccgcg ctgagggagg gtggaaaact ggtgtcgagc gtgagctgcg cgttggtctg
     5041 ccggctgtcc acgtacggcc tgcacgggcc ctggttcgag gagatgaggc acgtcgtgtt
     5101 ctgcgtatac gagtaccggg cggtggtgcg aatatcggtc ttgaggcgca cgagcgcggc
     5161 cggcggacgc caggcgaacg cgaggctggc gttctgctgg gtccgcatgt tgcgaaaggt
     5221 gttccccgcc gtcacctgct cggtccgcaa ccgcgtgccg tcgaaggacg tcaggacccc
     5281 atgcgcccac gaggctgtca ccccaggggc gagcgtgtgt tcgtccatcc gggtgacgct
     5341 ctccgtcccg cctcccccag agacaccgct gagcgtcgac tgccggttct cggtctgccg
     5401 gtgccgcacg ctgagttgcg ccgtgagccc ggacaggacc cgaccgatcg tcgtccgcgg
     5461 cggagagaac gtccagctca cgttccccga gggccactcg cggctggacg tcgtggtggg
     5521 aacctgaccg tcgaaccgca gcagccaggt ggtcccgccg gtccgctggt agaggcccgt
     5581 cacgcgaatc cccagcggca ggctcaccgt gccggcggcg cgccggctat tgttgtccgt
     5641 cgctccggtg gcgggaatgt cgcgctggct gcgaagcgcg ccgagcccga cgagccccag
     5701 ctggtaggtg agcgagggcg ccgccgcgag gctgttgtag atcgatgtcc ggtcgcgatt
     5761 gaacccgagg tcgagggagg tgagcgctcc cagggcccgg gcgatgggcg aggagtcacc
     5821 gaagatgccg cgccccagac ggccgagatc cagctgggtc cccagctcga cccgctgcga
     5881 gttgctgaag gccgtcggca gatggaaggc acccgagtct cctgccgtgc gaaccggggc
     5941 gcgtccgttg gcgtcgcgcg tgagcgagaa gcccgtggtc agcgagacgc gcgggctgag
     6001 ccagggcagg aaccgcggcg tcatgccgta gaacgtcccg atggtgcgct gtgtctccat
     6061 gccgacgtcc actcccagca acgacttgcg ggcgagacgc gcgacgcgcg cgatgctgga
     6121 ggagtctccg tagtcgcgca ggtcccgggt ggaggccagg tcaccgcgca cttgcacgtt
     6181 ggacagcggc agcaggtcga gcgaggcgga attgcgccac acgcgcgtga gcgagcgcgc
     6241 cgggaggaga tggcggtcgc cggtatcggc caccgggatc cggaagacgg tctggctggc
     6301 gtcgccgccg gacagggtgg agcgcagccg gatggacgtg ggactgagcc gcaggcgcgc
     6361 ccgttcgagg ccgtcggcga agccgctctt gccgatgaag ggcggcagct tacgcaccag
     6421 ggcgacgagg aaccgcgggg ccgccggaat cgtcgcccct ctgggctgca ggccgtagtc
     6481 cacgctgacc gcatagttgc gcgcggtcgc cgccgacagg ctggaacggt cgtccccgga
     6541 cgtgtaggcg gcggtgagcg acaacgggtc ggcgagccag cgggccggtc cggctgaggc
     6601 gcggcgcacg cgccgcagcg agaaggcgaa actgcgcgcc cgcgtgaggg gcgttcgcag
     6661 cccggccagg gcggcgccgc gcagatcggt gcccgcgagg aatacgggct cgttcgcaat
     6721 ggcggcgtag cgcatctgga gcggcgccgc gagcccccac tgcgggggca gcagccgctc
     6781 gagatggagc gtggtgacga ggctgtacgc ctgattcgtc tggtagctcg ggtcctcgcc
     6841 cagctggcgg aaattcccgt cgcggcgcga cacgtcgagg ctcacgtcgg cgagattcgc
     6901 ggcggtgagg gcgacgttca gcgccgccgc cgtgccggcg ttctggacga cgtcggagag
     6961 acggatgtcg tcgacccaga cttcggcctg gtcgatgaac gtcgcggcgc ccacgcgcca
     7021 gatccccgcg gcgagctcct gcaccgccgc caggttcggt ggcgcaccgc ccgggacctt
     7081 cacgtgggcg atataggctc cgtcgcacat cacgtaggcg gtgtcgtccg gtacgagcgg
     7141 gggggcgggg catcccggat acacgtgcgg cgggtccccg gcgagccagg cctgctggat
     7201 gtgcgtgcgc agcgccaccc accgatccag cgcgatcacc acctcgggat cccaggacgc
     7261 ggtgcgcgcc ggcgtgtggt agaagtagaa gttgtcggcg tccttcccca ctttgagaaa
     7321 gaactccaag tccccgtcct cccagcccgg cccgcgcccc cgcgcccaca cgcggagctg
     7381 gcggtacttg aggaagttct tgtcgccctc ggcgccgaac cgggtgaagg cctcggcgcg
     7441 ctgaccgagc tgcagtcccc gcgccagcag ccgcagggac tgctcgttga tctgcgtcgt
     7501 ccccacctga aagctctggt cctggcgcgc cgcctcgttt cccacgccgg ggggcggcgt
     7561 gtagcccagg tcgtgattct cggtgctcac caccgacgcg acgacctcgc ccagcccggt
     7621 ggcgcgatcg ccccccacgc cggccagcgg cgtttccgcc cgcttcagcc aggtcgcccc
     7681 cacgagccgg acccgcgaca gcgcgaagta gacctgcgga tccggatcac ccggggccgc
     7741 cgtctgcggc gcgagcaccg tgatccgcaa cgtctgcacc tgccgcaggt tgggcgaccc
     7801 cacctgcagc gtgtcggtgc gaaacgggat gcgaaacagt cgccatccgg acgctcctct
     7861 cgaggtctgg accatgccgc cgtcgcgcac gtagtagcga tcgtcgccga ccgggaacac
     7921 gaagcggacg aagtcctcct gggtcttggc cccgacggcc acgtccagct gtgcgtcgcc
     7981 gtcctggtcc tcggtatcca cggcgccgtt gtgccggccg cagcgcgagc gcgggtcacc
     8041 gaacaggtag ctcacgatct gcccgttcac cgaggcgctg cacagcggca actcggggat
     8101 cacctggcct gtggcggagt ccacgatgtt ttccacccgg tcgctcagga tgccctcgtc
     8161 gttgaccgaa gcgctccacg agtgagtgac cgggtcgcgc tcggtgtcga gacggcccac
     8221 ccccgcgatc cgcactccgc tgtacgtcgt gtcgccggcg ggactcacgc gcagcgtctc
     8281 gggcacgaag gcgagcgcgt cctcgaacac ggacccgaag tcgaacagca ccgccgtccg
     8341 gttggtcttc gcggtccgca gctcgtcctc ccacacccag aactcgaggt actcgacgcg
     8401 cgacaggtcg atccccgagg tggacaacgt ctgggtgatg gagcgccagc gcgtggtgag
     8461 ccgggcgttg ttgcccgacc cggggcggcg ccagttcggg atgccggtgc gcgagtcggc
     8521 caggcccatc atggtgtccg gcttgagcat cagccagagg acgggctccg ccgactgcac
     8581 ctggccggtg agcacgatgg tgggatcgat ctgcgtcgcc aggaactggg ccacgcgtcc
     8641 gctggagtcg gccagcggca gcgactgcca cgtgagcggg acggcgtcca gcgtgtcgaa
     8701 gccggtcgcc ggaatgtcga aggccgtggc gccacggctg gatgagggga cactgcccca
     8761 gtgccacgcg ttctcgtcca gggagatgaa gcggccggcc tgggcctcga actcctcgag
     8821 ataggcctgg ccgaggggat tgggacgggg gcgggacagg gctacctcgc ccgacacggt
     8881 gaggaaggac ggcgcgtcgc tccgggcgcc ggggatgagg tggacggccc gggtgatcca
     8941 ctccggctgg aagcgcagat tggcgctcac gccgccgatg aagccggagg ccggctcgaa
     9001 gccgagctgc gggcgggtga agatggtctg ctccttctgg aacagccccg tcaggtgcac
     9061 ctggcccagc tgacccaggt cgtaggtggc cgccatgccg tagatcgacg tgggcgcgac
     9121 cgcgaacgac g
//
LOCUS       DBNGZW010000564         3256 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1483175, whole genome shotgun sequence.
ACCESSION   DBNGZW010000564 DBNGZW010000000
VERSION     DBNGZW010000564.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3256)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3256)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3256
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1483175"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1586)
                     /locus_tag="AC16JC_13385"
     CDS             complement(<1..1586)
                     /locus_tag="AC16JC_13385"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5165737.1"
                     /translation="MVPVIEQLREHLAPRYQVERQIGAGGMARVYLATEQHPRRSVAI
                     KVLEPDIASRLLYDRFIREVEVSSRLTHPHIVPIFAAGEAGGLLYYIMPYIEGESLRH
                     RLVKQGRLPLEEAVHVVLDIADALAYAHAQGVVHRDIKPENVLMSGGHPVVADFGVAR
                     AISAAGGETLTQTGHAVGSPGYMSPEQAMGGEVDARSDIYSLGCLFFELLTGELPARA
                     GFGDVANWEVLGTSGVLRGASTGEGRAVKHAISRALAPLPADRFATVTEFADALGGSA
                     HRPSLPSRGRFASRRSWRGAVLALVLIAAAVGLALIGRRGSALVPGRVVVDVIENRTG
                     DSALDPLGHMAADWVTQGLTETGLVEVVPSVTVIATTATPTSPVPGAGGAAGIRALGR
                     ETQARTVVSGAYYRQGDSLRFQIDIADARDGKVMRALDPVVGPVANPLVAVEKLRERV
                     MIALGTLFNPKLAGWAAAASQPPDYAAYEQFVEGLDRFARFDYPGARDHFDRAAAGDS
                     TFSVAMIWSAAARLNLGEHGG"
     gene            1684..2739
                     /locus_tag="AC16JC_13390"
     CDS             1684..2739
                     /locus_tag="AC16JC_13390"
                     /inference="COORDINATES: protein motif:HMM:NF027640.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HIR5165738.1"
                     /translation="MTLPRGSPIVRLFAYYVLLAGLVWAVLSLFPGIPELEQRFREVS
                     ALGAGSRGRSFTDVSPGLETLSQGDLVLITLLASLAALALVVPVAYVYMVTKRRRGYD
                     QSVVQTVIILPMAVAGTVILVQNSIALAFALGAVMAAVRFRNTLKDTKDAVYIFLALA
                     VGVAAGVFAPSVAATMSVVFNLVVLALWYFNVGNIYVDQLGRVQPLRQGEALLGPGKS
                     GEILAVGDPRLLDALTPAELDEVADRAARLQAYIAARSGDTKKKRFTAVVLVHTTDPK
                     AAQQPVELVLEEQTKRWRLAEILPSVEGTTTLEYMVRLKEDSEAGALLEHLKRRGAPS
                     VVAAEYRSLRGLKPAEQ"
     gene            2736..>3256
                     /locus_tag="AC16JC_13395"
     CDS             2736..>3256
                     /locus_tag="AC16JC_13395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165739.1"
                     /translation="MSATRWVPWIMLAWAFTGTPVPAGAQRADSGPPRARVQLAAGER
                     YRAGWLHRLLLGAHYRDLWATPLSVEVLDLATFAGGLTPTACGGRRQTKSVRLRGADR
                     LEYVFRSVDKDPTLALPPELRRTFARDLLQDQISSAHPGAPLVVAPLLEATGVLHATP
                     RLYALPGDPRLTP"
BASE COUNT          481 a         1166 c         1124 g          485 t
ORIGIN      
        1 ccgccgtgct ctcccagatt gaggcgggcg gccgccgacc agatcatggc cacactgaac
       61 gtcgaatctc ctgcggcggc ccggtcgaaa tgatcgcggg cacccggata gtcgaaccgg
      121 gcgaagcgat ccagtccctc gacgaactgc tcataggccg cgtagtcggg cggctgactc
      181 gccgcggcgg cccagcccgc gagcttcggg ttgaagaggg tccccaacgc gatcatcacc
      241 cgctcacgca gcttctcgac cgcaacgagg ggattggcaa ccggccccac caccggatcg
      301 agggcccgca tcaccttgcc gtcccgggcg tccgcgatgt cgatctgaaa ccggaggctg
      361 tcgccctgcc ggtaatacgc ccccgagacg acggtccggg cctgcgtctc ccgccccagc
      421 gcacggatcc cggcggcacc gccggctccg gggaccgggg aggtcggcgt ggcggtcgtg
      481 gcgatcacgg tcaccgaggg gaccacctcg acgagcccgg tctcggtcag tccctgcgtc
      541 acccaatccg ccgccatgtg accgagggga tcgagtgccg aatcccccgt gcggttctcg
      601 atgacgtcga cgaccacccg tccagggacg agtgccgagc cgcgtcgccc gatcagcgcc
      661 agtccgaccg cggccgcgat cagcaccagt gccaggacgg cgcccctcca gctccggcga
      721 ctcgcgaacc tgcccctgct cggcagactg ggacggtgcg ccgatccgcc gagcgcatcg
      781 gcgaattcgg tgaccgtggc gaagcgatcc gccggcaggg gcgcgagcgc ccgggagatc
      841 gcgtgcttca cggcgcgccc ctcgccggtg ctggcaccgc gcagcacacc gctcgttccc
      901 aggacttccc aattggcgac atcgccgaac ccggcccggg cgggcagctc gccggtgagc
      961 agctcgaaga acaggcagcc caggctgtag atgtccgagc gcgcgtccac ttcgccaccc
     1021 atggcctgct ccggactcat gtagcccggc gagccgacgg catgtcccgt ctgcgtgagg
     1081 gtctcgccgc cggcggcgct gatcgcgcgg gcgacgccga aatcggctac cacggggtgg
     1141 ccgccggaca tcaggacgtt ctcgggcttg atgtcgcggt gcaccacccc ctgcgcatgg
     1201 gcataggcga gcgcgtcggc gatgtcgagc acgacatgca ccgcttcctc gagcggcagc
     1261 cgcccctgct tgacgaggcg gtgccggagc gattcgccct cgatgtacgg catgatgtag
     1321 tacaacaggc cgccggcctc gccggccgcg aagatcggaa cgatgtgggg gtgggtgagc
     1381 cgggaggaca cctccacctc ccggatgaaa cggtcgtaca gcaggcggct ggcgatgtcc
     1441 ggctccagga ccttgatggc cacggagcgt cgcggatgct gctcggtggc cagatacacc
     1501 cgcgccatgc ccccggcccc gatctgccgt tccacctggt agcggggagc gagatgctca
     1561 cgcagctgct cgatcaccgg gaccatgggg tgaagctagc gagcggctca ccgtcaggcg
     1621 agtgtgattg ggtgaagcgg tcgccctctg gccgaatcta ctgcgcacga tagtatgagc
     1681 ccgatgacgc tgccccgcgg ctcgccgatc gtgcggctct tcgcctacta cgtcctcctg
     1741 gcgggcctgg tctgggcggt gctgtcgctg ttccccggca ttcccgagct cgagcagcgg
     1801 ttccgggagg tatcggccct gggcgccggg agtcgagggc gcagcttcac ggacgtgagt
     1861 cccgggctgg agacgctgtc gcagggcgac ctggtgctga tcacgctcct cgccagcctc
     1921 gccgccctcg cgctcgtggt gcccgtggcc tacgtctaca tggtcaccaa acgacgccgt
     1981 ggctacgacc agtcggtggt ccagaccgtg atcatcctgc ccatggccgt cgccggcacc
     2041 gtgatcctgg tccagaacag catcgcgctc gccttcgcgc tcggggccgt gatggccgcc
     2101 gtgcggttcc gcaacacgct caaggacacg aaggacgcgg tctacatctt tctggcgctc
     2161 gcggtcggcg tcgcggccgg cgtgttcgcc ccttcggtgg ccgcgacgat gtccgtcgtc
     2221 ttcaacctgg tcgtgctggc gctgtggtac ttcaatgtcg gcaacatcta cgtggaccag
     2281 ctcggacggg tccagccgct gcgccagggc gaagccctgc tgggcccggg aaagtccggg
     2341 gagatcctgg cggtaggtga cccccggctg ctggatgccc tgacgcccgc cgagctcgac
     2401 gaggtggcgg accgggcggc gcggctccag gcgtacatcg ccgcgcggtc cggggacacg
     2461 aagaagaaac gcttcaccgc cgtcgtgttg gtgcatacca ccgacccgaa ggcggcacag
     2521 cagccggtcg agctcgtgct ggaggaacag acgaagcgct ggcggctcgc cgagatcctg
     2581 cccagcgtgg aagggaccac gacgctcgag tacatggtgc ggttgaagga agactcggag
     2641 gccggcgccc tgctcgaaca cctcaagcgc cgtggcgctc cgagcgtcgt ggcggcggag
     2701 tatcgctcgc tgcgaggact caagccggcc gagcagtgag cgcgacacgg tgggtcccgt
     2761 ggatcatgct cgcctgggcg ttcacgggga ccccggtccc tgccggcgcg caacgcgccg
     2821 attcgggacc gccacgagcg cgcgtgcagc tggcggcggg cgagcgatac cgcgccggct
     2881 ggctgcaccg cctgctgctc ggcgcgcact accgcgatct gtgggccaca cccctgagcg
     2941 tcgaggtgct cgatctggcg accttcgccg gcggattgac gccgaccgcc tgcggcgggc
     3001 ggcgccagac caagtccgtc cggctgcgcg gggccgaccg cctcgagtac gtcttccggt
     3061 cggtggacaa ggacccgacc ctggccctcc caccggagct gcgtcgcacc ttcgcgcgcg
     3121 acctgctgca ggatcaaatc agctccgcgc acccgggggc tccgctggtc gtcgcgccgc
     3181 tgctcgaggc gaccggcgtg ctccacgcga ccccgcggtt gtacgccctg cccggcgacc
     3241 cccgtctgac cccgtt
//
LOCUS       DBNGZW010000565         1861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1552868, whole genome shotgun sequence.
ACCESSION   DBNGZW010000565 DBNGZW010000000
VERSION     DBNGZW010000565.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1552868"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(244..1653)
                     /locus_tag="AC16JC_13400"
     CDS             complement(244..1653)
                     /locus_tag="AC16JC_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HIR5165740.1"
                     /translation="MRLLHTAATTHASFDDPNLVSHAGLIPAVRLAQNIGLEELARQH
                     VRVTAKVGASPGLKIGALVAGMVAGADTIDGMDLLRHGAFPATVGGIRAPSTLGSFLR
                     AFDHGNVRQLAAVHRRVLVELATQAPLLPGADTVAFIDVDSVQRRVYGGTKQGAAFGH
                     AKIASKSLLVRGLNILAATVSTPLAAPVVAAARLRGGNAGSARGAASLVAEAITTARL
                     AGATGLLVVRADSAYYAGAFVAACRRNGARFSVTVRMDPKVKRAIAAIPEDAWTAIKY
                     PNAIYDEQTGEWISDAQIAKVPYTAFASNRAHRTDGELIVRRVRRLNPKGTPQGQGEL
                     FATYRYHAVFTDSPFQLVQAESDHRGHAIVEQVFADLIDGPWAHLPSAAFNANAAWVQ
                     LAITAHAMTRALGVLASTRHALARGATIRAELVQVAARPARSGRDSITWHLPENWPWQ
                     DAWLGLFEATHRAPPARAA"
BASE COUNT          252 a          631 c          690 g          288 t
ORIGIN      
        1 ggcacccgca gaccgatggc accgttggtt gcgcggcggc ctcgggtgat ccgttcaccg
       61 tgctgggtga tgatgtcgta gctgagcctg gatccaccgc ccgaattcgg gtgatcattt
      121 caccaaaggc agttgatctt cgccgggcga tttcggcatt cgggcatgcg gaatcggccg
      181 gcgtctcatc cggcgggtcc accgtggtct ccggtcgtgc cccttgcggg cgggcggtca
      241 gcgtcaagcc gcccgggccg ggggtgcccg gtgggtggcc tcgaacaggc cgagccaggc
      301 gtcctgccag ggccagttct cgggcaggtg ccaggtgatg ctgtcgcggc cgctgcgggc
      361 cgggcgggcg gcgacctgga cgagctcggc ccggatcgtc gcgccccgcg cgagggcgtg
      421 ccgggtcgag gccagcacac ccagcgcccg ggtcatggcg tgcgcggtga tggcgagctg
      481 gacccaggcg gcgttggcgt tgaacgccgc ggatggcagg tgcgcccacg gtccgtcgat
      541 caggtcggcg aagacctgct cgacgatggc gtggccgcgg tggtcggact cggcctgtac
      601 cagctggaag gggctgtcgg tgaacaccgc gtggtaccgg taggtggcga acagctcacc
      661 ctgcccctgc ggggtgccct tcgggttgag ccggcggacc cgtcgcacga tcagctcgcc
      721 gtcggtgcgg tgggcccggt tggacgcgaa cgcggtgtag ggcaccttgg cgatctgggc
      781 gtcggagatc cactcaccgg tctgctcgtc gtagatcgcg ttcgggtact tgatcgcggt
      841 ccaggcgtcc tcggggatgg cggcgatggc ccgtttgacc ttcgggtcca tccgcacggt
      901 gaccgagaac cgggccccgt tgcggcggca cgcggcgacg aacgcccccg cgtagtacgc
      961 ggagtccgcg cgcaccacga gcaggccggt ggccccggcc agccgggcgg tggtgatcgc
     1021 ctcggcgacc agggaggccg caccccgggc ggagccggcg ttaccgccgc gtagccgggc
     1081 cgcggccacg accggcgcgg ccagtggcgt tgacacggtg gcggccagga tgttcagccc
     1141 gcgcaccagc agcgacttcg aggcgatctt ggcgtggccg aacgcggcgc cctgtttggt
     1201 gcccccgtac acccgccgct gcaccgagtc gacatcgatg aacgccaccg tgtcggcgcc
     1261 gggcagcagc ggggcctgcg tggccagctc gaccaggacc cggcggtgca ccgcggcgag
     1321 ttggcgcacg ttgccgtggt cgaacgcccg caggaagctg cccagcgtgg atggggcgcg
     1381 gatcccgccg accgtggccg ggaacgcgcc gtgccgcagc aggtccatcc cgtcgatggt
     1441 gtccgcgccg gcgaccatcc cggccaccag cgcaccgatc ttcaacccgg ggctggcgcc
     1501 gaccttggcg gtcacccgca cgtgctggcg ggccagctcc tccaggccga tgttctgggc
     1561 cagccgtacc gcgggtatca gcccggcgtg cgacaccaga ttcggatcat cgaaactggc
     1621 gtgggtcgtg gccgcggtgt gcaacaatcg catcccagag gtgccctttc gatcctggca
     1681 ttgcgagctt cgctaacccg cattctccca ggtcagaggg cgcttctgct gttgcgacac
     1741 gcttccgagc acaatccggg cggtggatcc aggctgagat caggaccgca cgtgcgcccg
     1801 gtgcgcacca tcccaaccgc tgacctgatg atttgcagcg cgttgacagc gcgaggcgat
     1861 a
//
LOCUS       DBNGZW010000566         4073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1483256, whole genome shotgun sequence.
ACCESSION   DBNGZW010000566 DBNGZW010000000
VERSION     DBNGZW010000566.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4073
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1483256"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1395
                     /locus_tag="AC16JC_13405"
     CDS             <1..1395
                     /locus_tag="AC16JC_13405"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014763.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HIR5165741.1"
                     /translation="DPRLRRLNDYLASVRERFGDYVELMVVDPQGRVLATSGRQIRPV
                     KMPPHWIEDLNSENSSFGDPYRDEALGSTLVVAAVPVHVPSGRFLGGLVAKISLGSVD
                     QIFRQYAPEDSGRIYLVTTDTSAGSAGTIVTGRGSGTRLLTKSYQQLVGGDSGIARYA
                     SYDGEHVLGSLSRDPEVLWGVVAETPASVAFSQVTRLRRLMIVLPAALLAGIGLIGFF
                     LGQLIVRPLQRLAKGAAKVAAGSLDVDLPVISSGEVGYLTQVFNHMVVRLREGREALD
                     AANETLRRKNEELERVSVTDGLTGLFNRRRLMEVLIDETRRSQRLKHTFAALMVDVDH
                     FKKYNDSFGHLAGDGVLTRVATMLRESTREVDFVARYGGEEFLVMLPETAMPEAQEIA
                     ERIRSRIAEESFSGRRITVSIGVAEFPLHGQTPEQVIAAADEALYEAKREGRDRVRRA
                     GLKLVRPRPKERAS"
     gene            complement(1392..2051)
                     /locus_tag="AC16JC_13410"
     CDS             complement(1392..2051)
                     /locus_tag="AC16JC_13410"
                     /inference="COORDINATES: protein motif:HMM:NF019844.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR5165742.1"
                     /translation="MTETVTVRDRDLEQRVARLQLQLLDRDALVEDLQARLDAASRDA
                     VRSMAKVQTLATRAEAASGMAEAQIVLQTLRRTARGSATPEVARATKMLSDAASEFDH
                     QNYGGALYLANQAKSQASVGRRRVTEGNGDPSAVRPGESMFAVPVALRTTGRCNLRDG
                     PGSTFKVLATLDGGVQLTGISYADQWMRVVDENGREGWVFWSLVQKRSDAGGAAADSG
                     R"
     gene            complement(2482..3858)
                     /locus_tag="AC16JC_13415"
     CDS             complement(2482..3858)
                     /locus_tag="AC16JC_13415"
                     /inference="COORDINATES: protein motif:HMM:NF017081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HIR5165743.1"
                     /translation="MKTVTLSRTTSSLVLLAGLAAVYFVAGKLGLRLAFVNASATPVW
                     PSAGIGLAALLVLGYRVWPAILLGAFFVNITTAGSLASSAAIAIGNTLEGVVGAYLVN
                     RYANGRDCFDRPGNIFRFAGLVAVMSATIAATWGATTLSLTGSAHWVEYGSIWLTWWL
                     GDAAADLVVTPVLVLWSRPSLPGWHRGHLLETAVLLVSLVIVSQLVFGGLFPDKNYPL
                     DFLCIPFLLWAALRFEPRAAAAALLLVAGIAVRGSLWGYGPFVRGTQNESLLLLQAFL
                     AVVSLMTLTVAAVVSARRRTAERLRLMSVTDPLTGLANYRQLISVLVAEINRSVRTGR
                     SFAVLFLDLDRLKRINDHHGHLVGSRAICRVGEALRASCRSIDTAARYGGDEFAVVLP
                     ETGETEARHVVGRVAQRLATDPESPPVSVSAGVALYPRDGETVATLLAAADRNLYQAK
                     GRVPESVT"
BASE COUNT          658 a         1418 c         1434 g          563 t
ORIGIN      
        1 gacccgcggc tccgccggct caacgattac ctcgcgtcgg tgcgcgagcg cttcggcgac
       61 tatgtggagc tgatggtcgt agacccgcag ggccgcgtcc tggcgacgag cgggcggcag
      121 atccgaccgg tgaagatgcc gccgcactgg atcgaagatc tgaactcgga aaactcgtct
      181 ttcggtgacc cctatcgcga cgaggcgctc ggctcgaccc tcgtagtggc cgccgtcccc
      241 gtgcacgtcc ccagcggccg gttcctcggc ggcctggtcg ccaagatcag cctgggatcc
      301 gtcgaccaga tcttccgcca gtacgccccc gaagattccg gccgcatcta cctcgtcacc
      361 acggatacca gcgcgggcag cgccggcacc atcgtgaccg gacgcggctc gggcacgcgg
      421 ctgctgacca agtcctacca gcagctggtc ggaggggaca gtgggatcgc ccgctatgcc
      481 agctacgacg gagagcacgt gctcggcagt ctgtcccgag acccggaggt cctctggggc
      541 gtcgttgccg aaacgccggc ctccgtggcc ttcagccagg tgacccggtt acgccgtctc
      601 atgatcgtgc tgcccgccgc cctgttggcc gggatcggcc tgatcgggtt tttcctgggc
      661 cagctcatcg tgcgaccgct acaacgcctc gccaagggcg ccgccaaggt cgcggccggc
      721 agtctcgacg tggacctgcc ggtcatctcc tcgggtgagg tgggctatct cacccaggtc
      781 ttcaaccaca tggtggtgcg cttgcgcgag ggtcgcgagg cactcgatgc ggcgaacgag
      841 accctccgca ggaagaacga ggagctggag cgggtctctg tgaccgacgg gttgacggga
      901 ctcttcaatc ggcgccggtt gatggaagtg ttgatcgacg agacgcggcg ctcccagcgg
      961 ctgaagcaca cgttcgctgc gctgatggtg gacgtggatc atttcaaaaa atacaacgac
     1021 agcttcgggc acctggcggg cgacggcgtg ctgacccggg tggccaccat gctgcgcgaa
     1081 tcgacccgcg aggtggactt cgtggcgcgc tacggcggag aagagttcct ggtcatgctg
     1141 cccgagaccg cgatgccgga ggcccaggaa atcgccgagc ggatccggag ccggatcgcc
     1201 gaggagtcct tctcgggccg gcgcatcacg gtgagcatcg gcgtggcgga gttcccgctg
     1261 cacggccaga ccccggagca ggtgatcgcg gcggccgacg aggcgctcta cgaggccaag
     1321 cgtgaagggc gcgaccgggt gcggcgggcg gggttgaagc tggtccggcc ccggcccaag
     1381 gagcgcgcca gctagcgacc cgagtcggcg gcggcgccgc ccgcgtcgct acgcttctgc
     1441 accaggctcc agaacaccca cccctcgcgc ccgttctcgt cgacgacccg catccactga
     1501 tccgcgtacg agatgccggt gagctggacg ccgccgtcga gcgtcgcgag gaccttgaac
     1561 gtcgacccgg ggccgtcccg gagattgcag cggccggtgg tgcggagcgc caccgggacc
     1621 gcgaacatgc tctcgcccgg acgcaccgcc gacgggtcgc cgttcccctc ggtgacccgg
     1681 cgccggccca cgctggcctg gctcttcgcc tggttcgcga ggtacagggc cccgccgtag
     1741 ttctggtgat cgaactcgct ggccgcgtcg ctcaacatct tcgtcgcccg ggcgacctcc
     1801 ggagtggcgc tcccgcgtgc cgtgcgccgc agcgtctgca atacgatctg ggcctccgcc
     1861 atgcccgagg cggcttcggc gcgcgtcgcc agcgtctgca ctttggccat cgagcgcacc
     1921 gcgtcccgac tcgccgcgtc gagccgcgcc tgcagatcct cgaccagcgc atcccgatcg
     1981 agcaactgca gctgcaaccg ggccacccgt tgctccagat cgcgatcccg caccgtgaca
     2041 gtttccgtca cgacacgagt cggcggcggg tcggcgctgg gcgcagggac cactgccggc
     2101 gcgcggtgag agcaggcggc aagcgccagc gcggcggcac acgcgagaaa cacgggggag
     2161 ggcgacgcca tggctggggg aagcgatggc aaaggcgggg ccaacggggc gccgacgatc
     2221 aactcgctgt cgtacaatgg tttatgctct ggagcctcgg ctgcacccgc ggcagacacc
     2281 acccctggcc tctcactccg ccagaatctt ggcgattccc cgctaccgca ccgtcagagc
     2341 ggccagggcg gcgagagcgg cgagcgcgag cagcggggcg agaatctccc gtacggcgaa
     2401 gcggggggtc ggcatggacc tcaagtctgg cgtgccgccg ggagacgcgc ggtgacccgt
     2461 gttaccggat caccgtgacg atcaggtcac ggattccgga acgcggccct tggcctgata
     2521 caggttccga tcggccgccg ccagcagagt cgccaccgtc tccccatcac ggggatacag
     2581 cgccaccccc gcgctcacgc tgacgggagg ggactcgggg tccgtcgcca agcgttgggc
     2641 gacgcggccg accacgtggc gggcctccgt ctcccccgtc tccggcagga ccaccgcgaa
     2701 ctcgtcgccg ccgtatcgcg ctgccgtatc gatggaccgg catgacgccc gcagcgcctc
     2761 ccccacccgg cagatggccc ggcttcccac gaggtgcccg tggtggtcgt tgatgcgctt
     2821 caggcgatcg agatccagga acagcaccgc gaacgagcgc ccggttcgca cggagcggtt
     2881 gatctcggcc acgagcacgc tgatcagctg ccggtagttg gccaggccgg tcagcgggtc
     2941 ggtcaccgac attaaccgca gccgctccgc cgtccggcgg cgggccgaga ccacggcggc
     3001 cacggtcagc gtcatgagcg agacgaccgc caggaacgcc tgcagcagca gcagcgattc
     3061 gttctgggtg ccgcgcacga acggcccgta cccccagagc gagccccgca cggcgatgcc
     3121 ggcgaccagc agcagcgcgg ccgccgccgc gcgcggctcg aaccgcaggg cggcccacag
     3181 caggaagggg atgcacagga agtcgagcgg atagttcttg tcgggaaaca gtcccccgaa
     3241 caccagctgg ctcacgatca ccagcgacac cagcagcacc gcggtttcga gcaagtggcc
     3301 acggtgccag ccgggcagcg acggccggct ccacaggacg agtaccggcg tcacgacgag
     3361 atcggccgcg gcgtcgccga gccaccacgt cagccagatc gacccatact cgacccagtg
     3421 ggcggacccg gtcagcgaca gggtggtcgc gccccaggtg gcggcgatgg tcgcgctcat
     3481 gacggcgacg agtccggcga agcggaagat gttgcccggc cggtcgaagc agtcccggcc
     3541 gttggcgtag cgattgacca ggtacgcgcc caccacccct tcgagcgtgt tgccgatggc
     3601 gatcgcggcg gagctcgcga gcgagccggc cgtggtgatg ttgacgaaga aggcgccgag
     3661 caggatggcg ggccataccc ggtaccccag gaccaggagt gcggcgagtc cgatgccggc
     3721 cgaaggccag acgggcgtcg cgctggcgtt cacgaacgcc agccgcagtc ccagctttcc
     3781 ggccacgaag tacacggccg ccagcccggc cagcaggacc aggctgctcg tggtgcggga
     3841 cagggtgacg gtcttcatgc ggccctacca tagtcgccgg cgagcgattc ccgggtgata
     3901 ctcgtcacgc ggcttcggga cggggcgcga ttccgggcct ggcccgggtc acttcgcggg
     3961 gacgacgccg ggcagcgcca ccggggaggg cgcggcggcg cggcggcggc cgaggcgccg
     4021 ccgcagcgcg tcgaggtagg tatagaacac cggggtgacg tacagggtga cga
//
LOCUS       DBNGZW010000567         5747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_598338, whole genome shotgun sequence.
ACCESSION   DBNGZW010000567 DBNGZW010000000
VERSION     DBNGZW010000567.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_598338"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1079
                     /locus_tag="AC16JC_13420"
     CDS             <1..1079
                     /locus_tag="AC16JC_13420"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5165744.1"
                     /translation="DDALGLVMLELNVLTDELKERRLGALEATALLRRVMAEIDVAVF
                     AFDEGEALRLVNAAGERLLAQSAERLLGRRAEELGLSQCLQGEAPRVIELGFPAARGR
                     WEVRRGAFRQGGRPHQFVVLADVSRALREEERRAWQRLIRVFGHEINNSLTPIKSVAQ
                     QLRALVARNGAAELSREDLQRGLDLIASRSESLTRFLASYAKLARLPSPKLGKVDVAA
                     WVQRVARLETRLPVEVVGGPPVTVRADGDQLDQLLINLIANAVDAALSTGGGVRIRWQ
                     PADGTLDVVVEDDGPGLPETANLFVPFFTTKPTGSGIGLVLSRQIAEAHGGRLSLANR
                     DGAHGCVARLQLPLVTDRKAASTR"
     gene            1209..2129
                     /locus_tag="AC16JC_13425"
     CDS             1209..2129
                     /locus_tag="AC16JC_13425"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5165745.1"
                     /translation="MAAPLIALAEPSQLEPQLPDSARRIAAATRQRYAIEREIGRGGM
                     SRLYLAWDRQASRQVAVKLLLQGPHTSLADRERFRREALIAARLEHPHIVPCHEFVCV
                     DGLALAVMRYVPGESLADALARGRRFDPSEVVALLAPIADALAHAHRDGVIHRDVKPA
                     NILLHGDDGWPFLTDFGIATLRTSEQSRSESAKAFGTPEFMSPEQAAGAWDADHRGDL
                     YSLGLVAYLLLAGRLPFRGTNALALAAQRVALEPPALGAVAPDVPPRLARVVDRCLER
                     DPRRRWRDAATLRRALLDALRPRRWRWWAR"
     gene            complement(2126..2275)
                     /locus_tag="AC16JC_13430"
     CDS             complement(2126..2275)
                     /locus_tag="AC16JC_13430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165746.1"
                     /translation="MSRRKKPPLTVRVPNPEAPAKRRLKVPSHKVHRSKKGYQRRPKH
                     PGRED"
     gene            complement(2272..2769)
                     /locus_tag="AC16JC_13435"
     CDS             complement(2272..2769)
                     /locus_tag="AC16JC_13435"
                     /inference="COORDINATES: protein motif:HMM:NF024273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HIR5165747.1"
                     /translation="MKRFSDLPAIVLGPLEGRPDPDWYQAPPGKWSPAQIVEHLALAL
                     EGCADGFEKRRARDPMFRRRLTGLERAGKLLVMHLGWFPPFLTAPSMTAPAPHVDRGA
                     AISHFRRGVERMEELSRLLLPARARDLFVKHPGMGDLTLPEWLEFHVIHSRHHARQIR
                     SRLRR"
     gene            complement(2958..3641)
                     /locus_tag="AC16JC_13440"
     CDS             complement(2958..3641)
                     /locus_tag="AC16JC_13440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165748.1"
                     /translation="MIPPILPILGAVAVAGGVAASVLFYERRRRAALEEYCLIRGYRF
                     ERERPGAEVPFAAAFGVFTHGHRRRWSATITGQVGGRPFTAFEYSYVTGGGQHSNRHR
                     FTAMLWESAEANLPRFTMVPEGVFRRLAQRFGAQDFDFDEDPEFSRAYQLQGDDERAV
                     RALFTPSRRAMLMTEAPDGRRAPKHHLAGAGTRLLWWRDGRLPGPDDLDQLIADGDRL
                     RRMFMEGRD"
     gene            3713..4885
                     /locus_tag="AC16JC_13445"
     CDS             3713..4885
                     /locus_tag="AC16JC_13445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR5165749.1"
                     /translation="MGRVRRYLVGVALGTAVLTAAFVPLPVLVDAVTGSATGDADLSL
                     PLSYVVTAPVSNLLDALTQLSMDRAVSLLIVWVVALVVIGVVRPGSRARRIVRVLVGA
                     SIPFLLAVAAVFLPRPVPRLVTSDSDVNVLDFHSHTAASHDGRHGWTPERVAAWHAAQ
                     GFDAAYITDHNVPFDGSNDAVIPLLPGTEWSVYRQHIVELGGLPPTDLAPYSHDTPGM
                     LGLFAALHARGGIAIASLPEYWRNHWEDLDQFVAAGLDGFEIVNCAPKALEFPSADRQ
                     AVITLAAQHDLLVDGVSDNHGWGTVTCVWNLAKEGSHGFRDNQVVARPLALAQGDLPA
                     WDAAYTQPWLMLRALSWPERVSWLTWIALITIWRGVPRRKGQGRGLGILARSLGGR"
     gene            4942..>5747
                     /locus_tag="AC16JC_13450"
     CDS             4942..>5747
                     /locus_tag="AC16JC_13450"
                     /inference="COORDINATES: protein motif:HMM:NF024603.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing domain"
                     /protein_id="HIR5165750.1"
                     /translation="MLSASLRLVLAATLGAAALSTTDELKVLRVSPQKTGEATEDVTV
                     TFDRPVAGGLDSTVDPRTIFQITPRVLGKLEWRDPVTIRLHPAAPLPAGVTFTVRIAN
                     TFQAMDGSRLAAPYSFSFRVKGPRVLDAYPVNQWINPKYLTPDVHFTMLLSAPADLAL
                     LAPLVSVDLGRCVRGKTIPLRPVRQRRIGRSDERWRYYGDYVEDSTRDLRRVVELAHA
                     EPLPLNCAATLRLPERVDSVTTIRSWLFHTYGPLAVDTVLCAWDTGRCPIG"
BASE COUNT          781 a         2093 c         1981 g          892 t
ORIGIN      
        1 ccgacgacgc cctcggtctc gtgatgctgg agctcaacgt cctcaccgac gagctgaagg
       61 agcgccggct gggcgccctc gaggccacgg cgctgctgcg gcgggtgatg gccgagatcg
      121 acgtcgcggt gttcgccttc gacgagggcg aggcgctgcg cctggtgaat gccgccggcg
      181 agcggttgct cgcccagagc gccgagcggc tgctgggccg ccgcgcggag gagctggggt
      241 tgtcccaatg tctccagggc gaggcgccgc gtgtcatcga gctggggttc cccgccgcgc
      301 gcgggcgctg ggaggtgcgc cgcggcgcgt tccgccaggg tggccgtccc caccagttcg
      361 tcgtgctggc cgacgtgagc cgcgccctgc gggaagagga acgccgggcg tggcagcggc
      421 tgatccgggt gttcggccac gagatcaaca attcgctcac gcccatcaag tccgtcgcgc
      481 agcagttgcg ggcgctcgtc gcccgcaacg gggctgcgga gctctcgcgc gaagacctgc
      541 agcgcggcct cgacctcatc gcaagtcggt cggagtccct cacgcgcttc ctcgcctcct
      601 acgcgaagct cgcgcgcctg ccatccccca agctcggcaa agtggacgtg gcggcctggg
      661 tgcaacgcgt ggcccggctg gagacgcggc tcccggtcga ggtggtcggc ggtccaccgg
      721 tgaccgtgcg cgccgatggc gatcagctgg atcagctgct catcaacctg atcgccaatg
      781 ccgtggatgc ggcgctgagc accggcggag gagtccgcat acggtggcaa cccgccgacg
      841 gcacgctcga cgtcgtggtc gaggacgacg gcccgggact ccccgagacg gcgaacctct
      901 tcgttccctt cttcacgacc aagcccaccg gctcggggat cggtctcgtg ctctcgcgcc
      961 agatcgccga ggcgcatggc gggcggctgt ccctcgccaa ccgcgacggc gcccacggct
     1021 gcgtggcccg cctgcagctc cccctggtga ccgaccgcaa ggcggcatcc actcgctagg
     1081 gcgtgcgctc gtgggacgcc gcgtaccgat aacggacagt tccgtcccgg cgcgaacagg
     1141 taacaccata ctgcaaaacg acttccggtg agtcggcggc ggcacgcccc ctgctttgcg
     1201 gaagggaaat ggctgcccca ctcatcgccc tcgccgagcc gtcccagctc gagccccagc
     1261 tccccgacag cgcacggcgc atcgcggcgg cgacccgcca acgctatgcg atcgagcgcg
     1321 agatcggccg cggcgggatg agccgcctat acctcgcctg ggaccggcag gcgtcgcgcc
     1381 aggtcgccgt caagctgctg ctccagggtc cgcacacctc cctcgccgac cgcgagcggt
     1441 tccgccgcga ggcgctcatc gccgcccggc tcgagcatcc ccacatcgtg ccgtgccacg
     1501 agttcgtgtg tgtcgacggg ctggcgctcg cggtgatgcg gtacgtgccg ggcgagtcgc
     1561 tcgccgatgc gctcgcgcgg ggacgccgct tcgatccgtc cgaggtcgtg gccctgctcg
     1621 ccccgatcgc cgatgcgctc gcgcacgcgc atcgcgatgg tgtcattcac cgcgacgtga
     1681 agcccgccaa catcctgctg cacggagatg acggctggcc gttcctcacg gacttcggca
     1741 tcgccacgct gcgcacctcc gagcagtcgc gctcggaatc cgcgaaggcg ttcgggacgc
     1801 ccgagttcat gtcgcccgag caggccgccg gagcttggga tgcggaccat cgcggcgacc
     1861 tctactcgct cggcctcgtg gcgtacctgc tgctcgccgg gcgcctgccg ttccgcggca
     1921 cgaatgcgct cgcgctggcc gcgcagcggg tcgccctcga gccgcccgcg ctcggcgcgg
     1981 tggcgccgga tgtgccgccg cggctcgccc gggtcgtcga ccgctgcctg gagcgcgacc
     2041 cgcgccggcg ctggcgcgat gcggcgacgc tgcggcgcgc gctgctcgac gcgctgcgcc
     2101 cacgtcgctg gcgctggtgg gcgcgctagt cttcgcgtcc cggatgtttg gggcgccgct
     2161 gatacccctt cttcgagcga tgcaccttgt gcgacggcac cttcagccgt cgcttggccg
     2221 gcgcctcggg gttcggcacc cggacggtga gcggcggctt cttgcggcgg ctcatcgccg
     2281 cagccggctc ctgatctgcc gcgcgtgatg ccgcgagtgg atcacgtgga attctagcca
     2341 ctcgggcagc gtgaggtccc ccatccccgg gtgcttcacg aacaggtcgc gcgcccgcgc
     2401 gggcagcagt agccgggaca gctcctccat tcgctccacg ccgcgacgga aatgcgagat
     2461 ggcggctccc cgatccacgt gcggggcggg cgccgtcatg gacggagcgg tcaggaacgg
     2521 cgggaaccag ccgaggtgca tcacgagcag cttccccgcc cgttcgagcc cggtcagccg
     2581 ccgccggaac atcggatcgc gggcgcgccg cttctcgaag ccgtcggcgc atccttccag
     2641 cgccagggcc aggtgctcga cgatctgcgc cggcgaccat ttcccggggg gcgcctggta
     2701 ccagtccggg tcgggacggc cctccagcgg accgaggacg atggcgggga ggtcggagaa
     2761 gcgcttcacg cgtcttcgcg ggtaaacccg cgactcggct gatctgtcac gcgaggtgac
     2821 acgtggtcgc tctccgtctc tgtgtcgcgg gtaaacccgc gactcggctg gtctgtcacg
     2881 cgaggtgaca cgcggtcgct ctcagcgaca gatagccgag gcgcgacttc agtcgcgaat
     2941 gaacgcgccg tgcgccccta gtcccggccc tccatgaaca tccgcctgag ccgatcccca
     3001 tccgcgatca gctgatcgag atcgtccggg ccgggcaagc gcccatcccg ccaccagagc
     3061 aatcgggttc ccgcgccggc cagatggtgc ttcggcgcgc gccgtccatc gggcgcttcc
     3121 gtcatgagca tcgcccgccg cgatggcgtg aacagcgcgc gcaccgcccg ctcgtcgtcc
     3181 ccctgcagct ggtaggctcg ggagaactcg ggatcttcgt cgaagtcgaa gtcctgcgct
     3241 ccgaagcgct gcgccaaacg gcggaagacc ccctcgggta ccatggtgaa gcggggcagg
     3301 ttcgcttccg ccgactccca cagcatcgcg gtgaaccggt ggcggttgct gtgctgtcct
     3361 ccccccgtca cgtagctgta ctcgaacgcc gtaaacggcc ggccgccgac ctgcccggtg
     3421 atcgtggcgc tccagcgccg ccggtgcccg tgcgtgaaga cgccgaacgc tgccgcgaag
     3481 ggcacttccg cgccggggcg ctcccgctcg aagcggtagc cgcggatcag gcagtactcc
     3541 tcgagcgccg cgcggcggcg gcgctcgtag aacagcaccg aggccgccac gccgccggcc
     3601 acggcgaccg cgccgaggat ggggaggatc ggagggatca tcggaacgag ggtcgaccgg
     3661 aagcgttcga ccccgtacct tgtgggcgac cgtccatgca cggattctag aggtggggag
     3721 ggtacgccgc tacctcgtgg gggtggcgtt ggggaccgcg gtgctcaccg cggcgttcgt
     3781 tccgcttccc gtgttggtgg acgcggtgac cggctctgcc acaggggacg cggatttgtc
     3841 gctgccgttg tcctacgtcg tgacggcgcc cgtttccaat ctgctcgatg cgctcaccca
     3901 actcagcatg gaccgggcgg tctctctgct catcgtctgg gtcgtggcgc tcgtcgtgat
     3961 cggcgtggtg cgtcctggaa gccgcgcccg gcggatcgtg cgggtgctgg tgggagcctc
     4021 cattccgttc ctcctggccg tcgcggccgt atttctcccc cgccccgtgc cgcggctcgt
     4081 gaccagcgac tccgacgtca acgtcctgga tttccattca catacggccg cctcgcacga
     4141 cggccgccac ggctggaccc ccgagcgggt ggcggcgtgg cacgccgccc aggggttcga
     4201 cgcggcctac atcaccgacc acaacgtccc cttcgacggc tcgaacgacg cggtgattcc
     4261 cctgttgccg ggcaccgagt ggagcgtgta ccgccagcac atcgtcgagc tcggcggctt
     4321 gccgccgacc gacctcgcgc cctattccca cgacacgccg ggaatgctcg gcctgttcgc
     4381 ggcgctgcac gcccgggggg ggatcgccat cgcctcgctg cccgagtact ggcgcaacca
     4441 ctgggaggat ctcgaccagt tcgtggcggc gggactggac gggttcgaga tcgtcaactg
     4501 cgcgcccaag gcgctcgaat tcccctcggc cgaccgccag gccgtgatca cgctggccgc
     4561 gcagcacgat ctgctggtcg acggcgtcag cgacaaccac gggtggggca ccgtcacctg
     4621 cgtctggaat ctggcgaagg aaggctcgca cgggttccgc gacaaccagg tcgtcgcccg
     4681 gccgctggcg ctcgcccagg gcgacctgcc ggcctgggac gctgcctaca cgcaaccctg
     4741 gctgatgctg cgggcgctgt cgtggcccga gcgggtgagc tggctgacgt ggatcgcgct
     4801 gatcacgatc tggcgagggg tgccgagaag gaaagggcaa ggacgcgggc tgggaatcct
     4861 cgcccgatcg ctgggcggac gctgacgttt gacaccgcgg gaggacatgc ttagatgcaa
     4921 taacctccac tgggcgcccc gatgctctcc gcctcactcc gcctcgtcct tgccgccacg
     4981 ctcggcgccg ccgccctctc caccaccgac gagctgaagg tgttgcgcgt ctccccccag
     5041 aagacgggcg aggccaccga ggacgtcacc gtcaccttcg accggcccgt ggcgggtgga
     5101 ctcgactcga cggtggaccc gcgcaccata ttccagatca ccccgcgcgt cctgggaaag
     5161 ctcgagtggc gcgacccggt gaccatccgc ctccaccccg cggccccgtt gccggccggc
     5221 gtcaccttca cggtccgcat cgcgaacacc ttccaggcga tggacggcag ccggctcgcg
     5281 gcgccctact ccttctcgtt ccgcgtgaag gggccgcgcg tcctcgacgc gtatccggtg
     5341 aaccagtgga tcaacccgaa gtacctgacg ccggacgttc atttcacgat gctgctgtcg
     5401 gccccggccg atctcgcgct gctggcgccg ctcgtgtccg tggacctggg ccggtgtgtc
     5461 cgggggaaga cgatcccgct gcggccggtt cggcagcgcc gcatcggcag gtcggacgag
     5521 cgctggcgct actacgggga ctacgtcgag gactcgacgc gcgacctgcg ccgcgtggtg
     5581 gagctcgccc acgccgagcc gctgcccctg aattgcgcgg ccacgctgcg cctccccgag
     5641 cgcgtggaca gcgtcaccac gatccgttcc tggctgttcc acacctacgg cccgctggcc
     5701 gtggacacgg tgctctgcgc ctgggacacc gggcgctgtc cgatcgg
//
LOCUS       DBNGZW010000568         8478 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1403322, whole genome shotgun sequence.
ACCESSION   DBNGZW010000568 DBNGZW010000000
VERSION     DBNGZW010000568.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8478)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8478)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8478
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1403322"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..875)
                     /locus_tag="AC16JC_13455"
     CDS             complement(30..875)
                     /locus_tag="AC16JC_13455"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR5165751.1"
                     /translation="MAGSYAHPEVLVDTAWVAEHLSDPQVRLLEVDVDTTAYDEGHLP
                     GAVGLHWRRDLETPVARDIASPEQIEGLLSVAGITPASTVILYGDNNNWFATYAWWLL
                     KYYGHADTRIMNGGRKKWMDEGRPMTKDVPAPKPTAYKTKAPDPSIRAARDEVLAAGR
                     TKSAALVDVRSPKEFSGELLAPEALPQEGAQRGGHVPGAANIPWGENVQADGTFKPAD
                     QLQALYEARGITSDRDVIAYCRIGERSSIAWFALKYLLGYPRVKNYDGSWTEYGSLVG
                     APIEQ"
     gene            complement(888..1163)
                     /locus_tag="AC16JC_13460"
     CDS             complement(888..1163)
                     /locus_tag="AC16JC_13460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165752.1"
                     /translation="MTAPVSHLVRLHYYRFFNAQGCALGSVDIVVEEALDPDAPAAAR
                     CVARPQHARLVTRPEYIVRAETVDAAVQRLVERLRARPLTDAFLPAQ"
     gene            1238..2146
                     /locus_tag="AC16JC_13465"
     CDS             1238..2146
                     /locus_tag="AC16JC_13465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002630514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR5165753.1"
                     /translation="MKRTRRGGVFVVLLGVTGVAPLSGQRLDSLRAAIEQRIARAPAR
                     AVGVYFRDLGTGDTLLVNADVRFHAASTMKVPVMIQLFRDRDAGTLSLDDSIPVTNTF
                     RSIVDGSPYQLDRADDSDSSLYALVGRKLPIRRLIELMETVSSNLATNLLIDHVGATR
                     ANATAHALGADSIVVLRGVEDGKAYRAGMNNTTTARDLGALFTALAQYRAASPAACRE
                     MLEILGRQHFTEGIPAGVPSGTRVAHKTGWIGGVVYHDAGVVYPASGSPYVLVVLTGG
                     IERDSVAYALVSDISRMLARNAAGGF"
     gene            complement(2219..2386)
                     /locus_tag="AC16JC_13470"
                     /pseudo
     CDS             complement(2219..2386)
                     /locus_tag="AC16JC_13470"
                     /inference="COORDINATES: protein motif:HMM:NF014427.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
     gene            2610..5459
                     /locus_tag="AC16JC_13475"
     CDS             2610..5459
                     /locus_tag="AC16JC_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019508829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5165754.1"
                     /translation="MRLSTAIATAESETEICRAVADGLRDEALGYDFVALLLVDAPTG
                     DRVLVASAGWAEAPAGLRVQPGQGLSERPLLDGRLHYTPQVTRDTRYLPTRNEGSEVD
                     VPVLINRELVGVLVVEGNRPDAFDAHDFEILTAAAQQAGIAIGRARLLAAERQRAHEQ
                     EALRATMADLSAQLELSTLLRAVLERAVALLRVSHGELAIYDAATQQLEIVASHNVGQ
                     RDTTGTRMAVGEGAMGHVARTREPLLIPNYLEWAGRSEQYAAVDFHAVMVAPLVVGGE
                     LVGAIAFMDRNPRRRFGEDDLRLLNLFAPQAAIAIANARLFTAAQEQRQYFAELVLNS
                     PVAIVTLDVGHRVVSCNPAFEKMYGYREAEVIGQNLDDLITTETTRSEAVGYTEQALA
                     RRAVKVISQRRRKDGSLVDVEVLGVPVIVNGRRVGLMALYHDITELLSARRDAEAANS
                     AKSQFLASMSHELRTPLNAIIGYSEMLQEDAAERGDGAAVPDLQKIHAAGKHLLALIN
                     DVLDLSKIEAGKMELHVETFPLAPLLEQVTTTVRPLVAKNGNRLELRTAADLGSMHSD
                     ETRVRQVLLNLLSNASKFTERGLITLEAERDGGTIVLRVRDTGIGMTPEQQARLFQAF
                     SQAEASTATKYGGTGLGLAISRRFCQLMGGDVTVESQPGHGSTFTVRLPADGPVEPAA
                     EITVPATAAAATVLVIDDEATARELVARSLEREGLRVVAAASGAAGLEAARTLRPDVI
                     TLDVMMPGMDGWTVLAALKGDPALAAIPVVMLSVADEQRLGFALGAAEYLTKPIERER
                     LAAVLRRYSGRPRPAGAAHHVLVVEDDGDVRRVLRRTLEGEGWAVAEAEHGQVALERM
                     AQRVPDLVLLDLMMPEMNGFEFLEALRERAAWRDVPVVVITAKELSDEERRRLNGDVE
                     RVVTKRGGGGEPGGGLEALAAVVREHVAARARVES"
     gene            5463..7643
                     /locus_tag="AC16JC_13480"
     CDS             5463..7643
                     /locus_tag="AC16JC_13480"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5165755.1"
                     /translation="MATSRRATPRRSGHAHRGAQADAARRQAALLRLTTGIAAATDER
                     EVYRSMVNGLHDEALGYNFLGAFLLDEASGDRVLQASVGWPDVPAQWRVHRGQGLSER
                     ALEDGQLHYTPDVTRAAGYLPSLASGSEVDVPVRVDGRTIAVLVVESSEPNAFGAEDF
                     EILTAAANQASIAIGRARLLAEERRRVDEHKALLDTMADLSSDLELPRVLQAVLNRAV
                     TLLGVTGGEVAIYEEQPKQLVVVASENIGKDSTGTRLSLGEGAMGHVARTREPLIIPS
                     YHEWLGRSGQYADVTVHSVMAAPLLIGRRLVGAIATVHSDPSRIFGAEDLRLLNMFAP
                     QAAIAIENARLYAAAQRQKQYFEDLVQNSPVAIVTLDRNHNVMACNPAFEKLFGYQQA
                     EAIGRNLDDLVATEATRSEAVQYSQEALSRPIHGIGRRRRKDGTLVDVEVLGVPVIVG
                     GERVGLMGLYHDITELLQARREAEAASTAKSQFLASMSHELRTPLNAIIGYSEMVEEE
                     IEELQQPQIAADVTKIRTAGKHLLSLINDILDLSKIEAGKMDLYLEPFAVATLIEEVA
                     ATVRPLVEKNANRLELRCAPDAGAMHADLTKVRQMLLNLLSNACKFTRNGTITLAVAR
                     EPGRGSDGPGAGGDRIVLRVTDTGIGMTPAQMERLFEAFAQADVATASKYGGTGLGLA
                     ITRRFCRMMGGDVSVESEPGRGSTFTIWLPAVTPAATPAPRSER"
     gene            7640..>8478
                     /locus_tag="AC16JC_13485"
     CDS             7640..>8478
                     /locus_tag="AC16JC_13485"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR5165756.1"
                     /translation="MSAPSLRSALLALSNRIAEARDEDAVCRAVVESLCHEAFGFDGV
                     GLYLAGTTAFEPALRASAGAFAPGERTRAELKLPLRVEQSAIGEVVVQRAGSRGGAFE
                     QGDLEILAAAANQAGIAIGRTRLLAADRQRIAEQRALLATLADLSGQLELDRLLQAVL
                     ERAVGLLDVSGGELAIFDEASRQLVIVASHNMGTNAIGTRMELGEGAMGHVAQTHEPL
                     IIPHYQQWASRSAQYAHGAIQTVMAAPLLIGDRLVGAIASVHSDPARTFGQGEMRQLN
                     LFAA"
BASE COUNT         1273 a         2842 c         3161 g         1202 t
ORIGIN      
        1 cgagcgcctc gagcttcacc atggcgacct cactgctcga tcggcgcgcc gacgaggctg
       61 ccgtattcgg tccacgatcc gtcgtagttc ttcacccggg gatagccgag caggtacttc
      121 agcgcgaacc aggcgatgga ggaccgctcg ccgatgcgac agtaggcgat gacgtcccgg
      181 tcggacgtaa tgccccgggc ttcgtacagc gcctggagct ggtcggccgg cttgaaggtg
      241 ccgtcggcct ggacgttctc tccccagggg atgttggcgg cgccgggcac gtgcccgccg
      301 cgctgcgcgc cctcctgcgg cagcgcctcg ggcgccagca gctcgcccga gaactccttg
      361 ggggagcgca cgtccaccag tgccgcggat ttggtgcgtc ccgcggcgag gacctcgtcg
      421 cgcgcggcgc ggatggaggg atcgggtgcc ttggtcttgt aagcggtagg cttgggggcc
      481 ggcacgtcct tggtcatcgg gcgcccctcg tccatccact tcttgcgtcc gccgttcatg
      541 atgcgcgtgt ccgcgtgacc gtagtacttg agcagccacc aggcgtaggt cgcgaaccag
      601 ttgttgttgt cgccgtacag gatcaccgtg ctcgccgggg tgatcccggc gacgctgagc
      661 aatccctcga tctgctcggg gctggcgatg tcgcgcgcca cgggggtctc gaggtcgcgc
      721 cgccagtgca acccgacggc tccggggagg tgcccctcgt cgtaggccgt ggtgtccacg
      781 tccacctcga ggaggcggac ctgcggatcg ctcaggtgct cggccaccca ggccgtatcg
      841 acgagcactt cggggtgtgc gtaggagcct gccatggtcg cctcctttca ctgggccggg
      901 agaaaggcgt cggtgagcgg gcgcgcgcgg agtcgttcga ccaggcgctg tacggcggcg
      961 tccaccgtct ccgcccggac gatgtactcc ggcctcgtca cgagccgggc gtgctgcggg
     1021 cgggcgacgc agcgggccgc cgcgggcgcg tcggggtcga gcgcttcctc gaccacgatg
     1081 tccacgctgc ccagcgcgca tccctgggcg ttgaagaacc gatagtaatg aaggcgcacg
     1141 aggtgcgaca caggggcggt cataggccca atacgatgcc ccgggtgggg aacgacgtca
     1201 aacgatcgtt gcccgccggc gggtatattc cggccccatg aaacgtaccc ggcgcggcgg
     1261 cgtgttcgtg gtgctcctcg gggtaaccgg ggtggcgccg ctgagcggtc agaggctcga
     1321 ctccttgcgc gcggcgatcg agcagcgcat cgctcgcgcg ccggctcggg cggtcggggt
     1381 gtatttccgg gacctgggca cgggcgacac gctgttggtg aacgccgacg tccgcttcca
     1441 cgccgcgagc accatgaagg tgccggtgat gatccagttg ttccgcgacc gggatgccgg
     1501 cacgctctcg ctcgacgatt cgatccccgt cacgaacacg ttccgctcga tcgtggacgg
     1561 atcgccctac cagctcgacc gggccgacga ttccgactcg tcgctgtacg cgctggtggg
     1621 gcggaagctc ccgatccgcc ggctcatcga gctgatggag acggtgtcgt ccaacctggc
     1681 caccaatctc ctgatcgacc acgtgggggc gacgcgcgcc aacgcgaccg cccacgcgct
     1741 gggcgccgat tcgatcgtcg tgttgcgtgg cgtggaggat gggaaagcgt atcgggcggg
     1801 gatgaacaac accaccacgg cgcgagacct gggcgcgctg ttcacggcgc tggcgcaata
     1861 ccgggcggcg tcgccggccg cctgtcgtga gatgctggag atcctgggcc gccagcattt
     1921 caccgaggga atccctgcgg gggtgccgtc ggggacccgg gtcgcgcaca agaccggatg
     1981 gatcgggggc gtggtttacc acgatgctgg cgtcgtctat ccggcgtcgg ggtcgcccta
     2041 tgtgctggtc gtgctgacgg gcgggatcga gcgggacagc gtcgcctacg cgctggtgtc
     2101 ggacatctcg cggatgctcg cccgtaacgc cgcaggtggc ttctagcgct gccccacgac
     2161 ccgcgtcggc accgcgcgat cgatcagcgt gccgtcgccc agccgcccgc gccgcgccag
     2221 tcacgccgtc cacggtggcg ctgatcgtcg ccgcaccggc accgacccct gtgaccagtc
     2281 cggtggccga taccgggagg tgcacggggt ccgagctcgt ccacagcacc gggcgacccg
     2341 cgaccggggc gggaaacgtg aagggggctt cacagcccgg gagcataagc aggacgagga
     2401 tcgaaccgct tcatcatgaa ccgccgtgcg tgtaccggat ttgtgatagg ccgggtgtgg
     2461 cggtcacgcg agatggcggc acggcggttg ccggggtcat attcccggac ctctcttcca
     2521 aggccgttcg actgcccaaa cgcaccccga cccgccctgc gcggcgcgcc gccgctcgtg
     2581 ctccgtctcg cgaccgcggc gcggcgctga tgcgcctcag caccgcgatc gccaccgcgg
     2641 agagcgagac ggagatctgc cgcgcggtgg ccgacgggct gcgcgacgag gcgctgggct
     2701 acgatttcgt cgcgctgctg ctggtcgacg cacccacggg cgatcgcgtc ctggtggcga
     2761 gcgcggggtg ggcggaggcg ccggcgggac tgcgcgtgca acccggtcag ggattgagcg
     2821 agcgcccgct gctcgacggc cgtctgcact acacgccgca ggtcacgcgc gacacccgct
     2881 acctgccgac ccggaacgag ggctcggagg tggatgtgcc cgtgctcatc aaccgcgagc
     2941 tggtcggcgt cctcgtggtg gaaggcaatc gtcccgacgc gttcgacgcg cacgacttcg
     3001 agatcctgac ggccgcggcg caacaggccg gcatcgcgat cggccgggcg cgcctgttgg
     3061 ccgcggagcg gcagcgggcc cacgagcagg aagcgctgcg cgccacgatg gccgacctct
     3121 cggcgcagct cgagctgtcc acgctgctgc gggccgtgct ggagcgcgcg gtcgcgctgc
     3181 tgcgggtgag ccacggcgag ctcgcgatct acgacgccgc cacgcagcag ctggagatcg
     3241 tcgccagcca caacgtgggc cagcgcgaca ccaccgggac ccgcatggcg gtcggggaag
     3301 gggcgatggg acacgtggcg cgaacgcgtg agccgctcct catccccaat tacctcgaat
     3361 gggccgggcg gtccgagcag tacgccgcgg tggactttca cgcggtcatg gtggctccac
     3421 tggtggtggg cggcgagctg gtcggcgcga tcgccttcat ggaccgaaat ccccggcggc
     3481 gcttcggcga ggacgacctc cggctgctga acctgttcgc cccgcaggcg gccatcgcga
     3541 tcgccaacgc gcgcctgttc accgcggccc aggagcagcg ccagtatttc gccgagctgg
     3601 tgctgaacag cccggtggcg atcgtgacgc tggacgtggg gcaccgggtc gtctcgtgca
     3661 atcccgcctt cgagaagatg tacggctatc gcgaggccga ggtgatcggc cagaatctgg
     3721 acgacctgat cacgacggag acgacgcgct cggaggccgt gggatacacc gagcaggcac
     3781 tggcgcgccg ggcggtgaag gtcatcagcc agcggcgccg caaggacggc agtctggtgg
     3841 acgtcgaggt gctgggtgtg cccgtgatcg tgaacggccg ccgcgtcggg ttgatggcgc
     3901 tgtatcacga catcacggag ctgctctcgg cgcggcgcga cgcggaggcg gccaacagcg
     3961 cgaagagcca gtttctggcg agcatgagcc acgagctgcg cacgccgctc aacgcgatca
     4021 tcggctacag cgagatgctg caggaagacg ccgccgagcg cggggacggc gcggcggtgc
     4081 ccgacctgca gaagatccac gcggcgggga agcatctgct ggcgctgatc aacgacgtgc
     4141 tcgatctatc caaaattgaa gcgggcaaga tggagctcca cgtcgaaacg ttcccgctcg
     4201 cgccgctgct ggagcaggtg acgacgaccg tgcgtcctct ggtcgcgaag aacggcaacc
     4261 gtctcgagct gcgcaccgcc gcggacctgg ggtcgatgca ctccgacgag acgcgcgtgc
     4321 gccaggtgct gctcaacctg ctctccaacg ccagcaagtt caccgagcgc ggtctgatca
     4381 cgctcgaagc cgagcgggac ggcgggacga tcgtgctgcg ggtgcgcgat acgggcatcg
     4441 gcatgacgcc ggagcagcag gcgcggctgt tccaggcgtt ctcgcaggcg gaagcctcca
     4501 cggcgacgaa gtacggcggc acggggctgg gtctcgccat cagccggcgg ttctgccagc
     4561 tgatgggcgg cgatgtcacg gtcgagagcc agccgggaca cggatcgacc ttcaccgtgc
     4621 ggctgccggc ggacggaccg gtcgagccgg ccgcggaaat cacggtgccc gcgacggcgg
     4681 ccgccgcgac ggtgctcgtg atcgacgacg aggcgacggc gcgcgagctc gtggcccgga
     4741 gcctcgagcg ggaagggctg cgcgtcgtgg ccgcggcgag cggcgcggcg gggctcgaag
     4801 cggcccgcac cctgcggccg gacgtgatca cgctggacgt catgatgccc ggcatggatg
     4861 gctggaccgt gctggcggcg ctgaagggcg atcccgcgct ggccgcgatc ccagtggtga
     4921 tgctctcggt ggcggacgag cagcggctcg ggttcgcact cggcgcggcg gagtatctca
     4981 ccaagccgat cgagcgcgag cggctggcgg cggtgctgcg gcgctacagt gggcgcccgc
     5041 ggcccgcagg cgcggcgcac cacgtgctcg tggtcgaaga cgacggggac gtgcgccgcg
     5101 tgctgcgacg gaccctggaa ggcgaggggt gggcggtcgc ggaggccgag cacggccagg
     5161 tcgcgctcga gcgcatggcg cagcgggtcc ccgatctggt gctgctcgac ctcatgatgc
     5221 cggagatgaa cggcttcgag ttcctcgagg cgctgcgcga gcgggcggcg tggcgcgacg
     5281 tgcccgtcgt ggtgatcacg gccaaggagc tcagcgacga agagcgccgg cgcctgaacg
     5341 gcgacgtgga gcgggtcgtc acgaagcggg gtggtggcgg cgagccgggc ggcggtctcg
     5401 aggccctggc cgcggtggtg cgcgagcacg tcgcggcccg tgcccgggta gaatcctagg
     5461 tcatggcgac atcacgccgc gcgacccccc ggcgctcggg ccacgcgcac cgcggggctc
     5521 aggccgacgc cgcgcgacgg caggccgcgc tgctgcgcct caccacgggg atcgccgccg
     5581 cgaccgacga gcgggaagtg taccgctcga tggtgaacgg cctgcacgac gaagcgctgg
     5641 gctacaactt cctcggtgcg ttcctgctgg acgaggcatc gggcgaccgc gtgctgcagg
     5701 cgtcggtcgg ctggcccgat gtccccgcgc agtggcgcgt gcaccgcggg caggggttga
     5761 gcgagcgggc cctggaggac gggcagctcc actatacccc ggacgtgacg cgcgcggcgg
     5821 gctacctgcc gagcctcgcg agcggctccg aggtggacgt gcctgtgcgg gtggacggcc
     5881 ggacgatcgc ggtgctggtg gtggagagca gcgagccgaa tgcgttcggt gcggaggact
     5941 tcgaaatcct caccgcggcg gccaaccagg cgagcatcgc gatcggccgc gcgcggctcc
     6001 tggccgagga gcggcggcgc gtggacgagc acaaggcgtt gctcgacacg atggccgacc
     6061 tctcctcgga cctcgagctg ccgcgcgtgt tgcaggccgt gttgaaccgc gccgtgaccc
     6121 tgctcggcgt aacgggcggc gaggtggcga tctacgagga gcagccgaag cagctggtgg
     6181 tggtggcgag cgagaacatc gggaaggact ccacggggac gcggctgtcg ctgggcgagg
     6241 gtgcgatggg gcacgtggcg cggacccgcg agccgctgat catcccgtcc tatcacgagt
     6301 ggctggggcg ctccgggcag tacgccgacg tgacggtcca ctcggtgatg gcggcgccgc
     6361 tgctgatcgg ccggcggctg gtgggagcga tcgccacggt gcattccgac ccgagccgca
     6421 tcttcggagc ggaggacttg cggctgctca acatgttcgc gccgcaggcc gcgatcgcga
     6481 tcgagaacgc ccgcctgtac gccgccgccc agcgccagaa gcagtacttc gaagacctcg
     6541 tgcagaacag ccccgtcgcc atcgtcacgc tggaccgcaa ccacaacgtg atggcgtgca
     6601 atccggcctt cgagaagctg ttcgggtacc agcaggccga ggcgatcggg cgcaacctcg
     6661 acgacctcgt ggcgaccgag gccacgcgct cggaggcggt ccagtactcc caggaagcgc
     6721 tgagccgccc gatccacggc atcggccgcc gccggcgcaa ggacggcacg ctggtggacg
     6781 tggaagtcct gggcgtcccg gtgatcgtcg gcggggagcg ggtcgggctg atgggtctct
     6841 atcacgacat caccgagctg ctccaggccc ggcgcgaggc cgaggcggcc agcaccgcca
     6901 agagccagtt cctcgcgagc atgagccacg agctgcgcac gccgctcaac gcgatcatcg
     6961 gctacagcga gatggtggag gaagagatcg aagagctgca acagccgcag atcgcggcgg
     7021 acgtgaccaa gatccggacg gcgggcaagc acctgctgtc gctgatcaac gacatcctcg
     7081 atctctcgaa gatcgaggcc ggaaagatgg atctgtacct ggagccgttc gcggtcgcca
     7141 cgctgatcga ggaggtcgcc gccaccgtgc ggccgctggt cgaaaagaac gccaaccggc
     7201 tggagctccg ctgcgcaccg gacgccggcg cgatgcacgc cgatctgacc aaggtccggc
     7261 agatgctgct caacctgctg tccaacgcct gcaaattcac gcgcaacggc acgatcacgc
     7321 tggcggtcgc gcgggagccg gggaggggga gcgacggccc gggagcgggc ggcgaccgca
     7381 tcgtgctccg ggtgaccgat accggcatcg ggatgacccc ggcccagatg gagcggctgt
     7441 tcgaggcgtt cgcccaggcc gacgtcgcga cggccagcaa gtacggcggg accgggctcg
     7501 ggctcgccat cacccgccgc ttctgccgga tgatgggcgg cgatgtctcg gtggagagcg
     7561 agccgggccg gggatccacc ttcacgatct ggctgcccgc cgtgacaccg gccgccaccc
     7621 ccgcgccgcg gagcgaacgg tgagcgcccc atcgctgcgc tccgccctgc tggcgttgag
     7681 caaccgcatc gccgaagccc gggacgagga cgccgtgtgc cgcgccgtgg tcgagagcct
     7741 gtgccacgag gcgttcggct tcgacggcgt ggggctgtat ctggccggca cgaccgcgtt
     7801 cgagcccgcg ctgcgggcga gcgccggcgc gttcgccccg ggcgagcgga cgcgcgccga
     7861 gctgaagctg ccgctgcggg tcgagcagag cgccatcggc gaggtggtgg tccagcgggc
     7921 tggaagccgc ggcggggcgt tcgagcaggg ggacctcgaa attctggcgg ccgccgcgaa
     7981 ccaggcgggc atcgccatcg ggcggacgcg cctgctggcc gccgaccgcc agcgcatcgc
     8041 cgaacagcgg gcgttgctcg ccacgctggc cgatctgtcg gggcagctgg agctggaccg
     8101 cctgctccag gccgtgctcg agcgggccgt cgggttgctc gacgtctccg gcggcgagct
     8161 ggccatcttc gacgaggcct cgcgccagct ggtcatcgtg gcgagccaca acatgggcac
     8221 caacgccatc ggcacgcgca tggagctggg cgagggcgcg atgggccacg tcgcccagac
     8281 ccacgagccg ctgatcattc cccactatca gcagtgggcc agccgctcgg cgcagtacgc
     8341 gcacggcgcg atccagacgg tcatggccgc gcccctcctg atcggcgacc gcctggtcgg
     8401 ggccatcgcg agcgtgcatt ccgacccggc gcgcaccttc gggcagggcg agatgcggca
     8461 gctcaatctg ttcgccgc
//
LOCUS       DBNGZW010000569         2681 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_598427, whole genome shotgun sequence.
ACCESSION   DBNGZW010000569 DBNGZW010000000
VERSION     DBNGZW010000569.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2681
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_598427"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1357)
                     /locus_tag="AC16JC_13490"
     CDS             complement(<1..1357)
                     /locus_tag="AC16JC_13490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165757.1"
                     /translation="MLAAVALLLALQSPSDSAAYLDPGARDLVARARLRRATAEEAIT
                     DYHARVQERISLGLRALRRDRTFFQREMAAQIAWHRYGPDTITMLGAREAIPVVYRDV
                     RLPEDLDTDAPDLAFEPGRGRIDVGIGDSEFVYDPLADGGEAQYRFQSGDTTVITLQD
                     GRTIRLLELRIIPRRDEFHLLTGSFWIEDEHAALVRAVFRPARAWDLERDLAGDPEDS
                     SDAKGIPGFLKPIRAEVRYITAEYALVDGKWWLPRLIAFDAVATAGSFLTTPLRYERV
                     FSDFDVTAAPGGGLAAADTSLRDSLDRDTMPPDSAAIIACQGRAGCRCTRRACRTAVV
                     IVPQDTASLLTSPSLPRSFDDDGAFLSSSEARELGRAVGLLPQTPWHAHAPSLRFGPG
                     GTGLMRYNRVEALSLGARMGFDFGRLTADVTARVGTADRWPNVEVGVEHPGSDVSLRV
                     AG"
     gene            1436..1924
                     /locus_tag="AC16JC_13495"
     CDS             1436..1924
                     /locus_tag="AC16JC_13495"
                     /inference="COORDINATES: protein motif:HMM:NF025892.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4442 domain-containing protein"
                     /protein_id="HIR5165758.1"
                     /translation="MPPNLPAPGQRLRSLWNRLSPYPSGKWLFSRILGRMAPYSGTIG
                     ALVEQLEPGYAKVSLADRRRVRNHLDSIHAVALVNLAEQASGLAVLMGLPSDVRGILT
                     GLTITYHKKARGRLVAECRCAIPAVTGSIEYEAVATIKDSGEEVVAQAVARWRLGPTK
                     PA"
     gene            1921..>2681
                     /locus_tag="AC16JC_13500"
     CDS             1921..>2681
                     /locus_tag="AC16JC_13500"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent flavin oxidoreductase"
                     /protein_id="HIR5165759.1"
                     /translation="MSLLDTPLSRSLGIEVPLLCGAMFPCSNPELVAAVSEAGGIGIV
                     QPLSLTYVRGYDLREGLRRIRRLTTKPIGMNVLIEQSSQAYLERMRRYLDVALEEGVR
                     FFVTSLGNPRWVVERAGPLGGLVYHDVTERKWAEKGLAGGVHGLIAVNNRAGGHAGRR
                     TAEQLLAELGDLGVPVVCAGGIGDADGFVAALRMGYAGAQLGTRFIATTECDAAPEYK
                     QAIVAARADDIVLTERVTGVPLAVIRTPYVERVGTR"
BASE COUNT          412 a          933 c          964 g          372 t
ORIGIN      
        1 atcccgcgac gcgcagcgac acatcggacc ccgggtgctc caccccgacc tccacattcg
       61 gccaccggtc cgccgtgccg acgcgggcgg tgacgtcggc ggtgagccgg ccgaagtcga
      121 atcccatgcg cgccccgagc gacagggcct cgacccggtt gtaccgcatg agaccggtgc
      181 cgcccggacc gaatcgcagc gacggcgcgt gggcgtgcca tggggtctgc ggcaacagcc
      241 ccaccgcccg tcccagctcc cgggcctcac tggaggaaag gaacgcgccg tcgtcgtcga
      301 aggagcgtgg cagggacggg ctggtaagca ggctcgccgt gtcctggggg acgatgacca
      361 cggcggtgcg acacgcacgc cgcgtgcacc ggcagccggc ccgtccctgg caggcgatga
      421 tcgccgccga gtcgggaggc atggtatcgc ggtcgagcga gtcccgcagg gacgtgtcgg
      481 ccgccgcgag accgccgccc ggcgcggcgg tcacgtcgaa atcggagaac acgcgctcgt
      541 agcgcaacgg cgtcgtgaga aacgagccgg cggtcgcgac cgcgtcgaac gcgatcagcc
      601 tcggcagcca ccacttcccg tcgaccagcg cgtactcggc ggtgatatag cggacctcgg
      661 cacggatcgg cttcagaaac ccgggaatgc ctttcgcgtc gctcgagtcc tctggatcac
      721 ccgccaggtc ccgctccaga tcccaggccc gggccggccg gaacacggcg cgcaccagcg
      781 ccgcgtgctc gtcctcgatc cagaaggatc ccgtgagcag atggaactcg tcgcgccgcg
      841 ggatgatgcg cagctcgagc aaccggatgg tgcgcccgtc ctgcagggtg atgaccgtgg
      901 tatcgccgga ctggaagcgg tattgcgctt cgccaccgtc ggcgagcgga tcgtagacga
      961 actccgaatc gccgatgccg acgtcgatcc ggccgcgacc gggctcgaac gccaggtccg
     1021 gcgcatccgt gtccaggtcc tccggcagcc ggacgtcgcg gtacacgacg gggatcgctt
     1081 cccgcgcgcc cagcatcgtg atcgtatcgg gcccgtagcg gtgccaggcg atctgcgccg
     1141 ccatctcccg ctgaaagaag gtccgatcgc ggcgcagcgc gcgcagcccg agggaaatcc
     1201 gctcctgcac ccgggcgtga tagtcggtga tcgcctcttc ggcggtggcg cgacgcaacc
     1261 gggcgcgcgc gaccaggtcc cgcgccccgg gatcgaggta ggcggcggaa tcggacggcg
     1321 actgcagggc gaggagcagg gcaacggccg cgagcatgca ccggaacata cgcccggcgc
     1381 ttcggggagt ttcggccttg gcgccggagg gtcgcccgat gagattccgg cacctatgcc
     1441 gcccaacctt cccgccccgg gacaacgcct ccgatccctc tggaaccgcc tgagccccta
     1501 tcccagcggc aaatggctgt tcagccgcat tctgggccgg atggcaccgt acagcgggac
     1561 gatcggagcc ctggtggagc agctcgagcc aggctacgcc aaggtctccc tggcggaccg
     1621 gcgccgggtg cgcaaccacc tcgattcgat tcatgcggtg gccctcgtga acctggccga
     1681 gcaggcgagc ggcctggcgg tgctgatggg actgccgtcc gacgtgcgcg gcatcctgac
     1741 cggactgacg atcacctacc acaagaaggc ccggggccgc ctggtcgccg aatgccgctg
     1801 tgcgatcccg gccgtcaccg ggagcatcga gtacgaggcg gtcgccacga tcaaggattc
     1861 gggcgaggag gtcgtcgcgc aggccgtggc gcgctggcgt ctcgggccca cgaagcccgc
     1921 gtgagcctcc tggatacgcc gctgtcacgc agcctcggga tcgaggtgcc gctgctgtgc
     1981 ggcgcgatgt tcccctgttc caatcccgag ctcgtggccg cggtgtcgga ggccggcggg
     2041 atcgggatcg tgcaaccgct gtcgctcacc tacgtgcgcg gctacgacct ccgcgagggc
     2101 ctccggcgca tccggcggct caccacgaag ccgatcggca tgaacgtgtt gatcgagcag
     2161 agctcccagg catatctcga gcgcatgcgc cgctatctcg acgtggcgtt ggaggagggc
     2221 gtgcgcttct ttgtgacctc gctcggcaac ccgcgctggg tggtggagcg cgccgggccg
     2281 ctcggcggcc tggtgtatca cgacgtgacc gagcgcaaat gggcggaaaa gggcctcgcg
     2341 ggcggcgtgc acggcctcat cgccgtgaac aatcgcgcgg gggggcatgc cggtcgcagg
     2401 accgccgagc aactgctggc cgagctgggc gacctgggag tgccggtggt gtgcgcgggc
     2461 ggcatcggcg acgcggacgg gttcgtcgcc gcgctccgga tgggctacgc cggcgcgcag
     2521 ctcggcacgc gcttcatcgc gacgaccgag tgcgatgccg ccccggagta caagcaggcg
     2581 atcgtcgccg cccgcgccga cgatatcgtg ctgaccgagc gcgtcaccgg tgtgccgctg
     2641 gcggtgatcc ggacgccgta cgtggagcgc gtgggtaccc g
//
LOCUS       DBNGZW010000570         3557 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_257465, whole genome shotgun sequence.
ACCESSION   DBNGZW010000570 DBNGZW010000000
VERSION     DBNGZW010000570.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3557)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3557)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3557
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_257465"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            181..729
                     /locus_tag="AC16JC_13505"
     CDS             181..729
                     /locus_tag="AC16JC_13505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR5165760.1"
                     /translation="MSRIPQIDPAGATGDTAAHMTTARKMFGGSVPNLFATAAQSPAA
                     VGAMLSFFASLGEGSLGAKAGEQIAIAIAQSNGCGYCLSAHTAIGSMVGLAPAALAAA
                     RRAQGTDPKTQALLTLALAVNQTRGHIDDATLAAARLAGLGDAEIVELVAHVALNVFT
                     NYLNNVAETKIDFPEVSLFAAA"
     gene            742..942
                     /locus_tag="AC16JC_13510"
                     /pseudo
     CDS             742..942
                     /locus_tag="AC16JC_13510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352572.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1348 family protein"
     gene            951..1622
                     /locus_tag="AC16JC_13515"
     CDS             951..1622
                     /locus_tag="AC16JC_13515"
                     /EC_number="1.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015438.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016661 - oxidoreductase activity, acting
                     on other nitrogenous compounds as donors [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HIR5165761.1"
                     /translation="MSLVVAGLWRYPIKSLAGEPIDAAELTPTGIPGDRVVHVRGPEG
                     VRTSRRHYRLLGLRGTLDASGRPLINGLPWDGEEALALVRRAAGEDAWLEAYDGAERF
                     DVLPLLVATDGAVAAFGRDIRRLRPNILIGGVDGLAERSWEGATLRIGGAVILLDSLR
                     VRCPMTTVDPDTLKRDKEVLRDIGRRFGGRLALNAAVARPGIVQRGDRVRYSAAGLHV
                     DEARR"
     gene            complement(1666..2166)
                     /locus_tag="AC16JC_13520"
     CDS             complement(1666..2166)
                     /locus_tag="AC16JC_13520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165762.1"
                     /translation="MSTADIRRAPRSALSVVLRAGVTTALVDGLFSSIVVVLYGSTVP
                     RLWQGVAAVLIGSGALSGGTPTVLLGLAMHFCVATTWSAIFVYAVSRSARIRGVLASP
                     GGVYKVAAVYGPAIWMVMSLVVIPALTHRPPRISYRWWVQWFGHIPFVGLPIAMSVRR
                     AQSRAT"
     gene            complement(2163..2411)
                     /locus_tag="AC16JC_13525"
     CDS             complement(2163..2411)
                     /locus_tag="AC16JC_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165763.1"
                     /translation="MPRFAAFLRGVMPMNAKMPDLKRAFEPAGCEDRSLSPHDRGVLS
                     AYVRHSRGQPVFMTLIEKTFGKALTTRTWDTVAKVARA"
     gene            complement(2495..3313)
                     /locus_tag="AC16JC_13530"
     CDS             complement(2495..3313)
                     /locus_tag="AC16JC_13530"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR5165764.1"
                     /translation="MAATDGLTNDRFPRSASYHHEWVRASVSGGANALWLVEWLATAL
                     DLRPGMKVLDLGCGRAASSIFLHREFGVQVWATDLWFDVSQNLQRIRDAGVEDGVFPI
                     HADARSLPFAAEFFDAAVCIDGYPYFGTDDLYLGTLARFLRPGAQLAIAGAGLMQEID
                     GPIPEHLREWWTPELWCLHSADWWRRHWERTGIVDVALADSMPEGWRRWLDWQRAIAP
                     DNRVEITAVEADAGRYLGYVRVAARRREGAALEAPLTSLATQYTRQPLLRDQER"
     gene            3460..>3557
                     /locus_tag="AC16JC_13535"
     CDS             3460..>3557
                     /locus_tag="AC16JC_13535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR5165765.1"
                     /translation="MRGFIGDIENRTEANQDFRRVLYTGPHMQLVL"
BASE COUNT          592 a         1263 c         1179 g          523 t
ORIGIN      
        1 cgatgtgtcg ctcttcgggc gcgccatgaa acagctcccg gacgaattgc tcgccccggc
       61 ctcgcgccgg aggtgagcgt cgggccggac acggcccgga ccgcagaggg gtagcgaggt
      121 gcttgtattg taccgatcgt tcggtacatt cctcactcac ccttgtccaa ggacccgcac
      181 atgtctcgta ttccccagat cgatccggcc ggcgcgaccg gcgacaccgc cgcccacatg
      241 accactgccc gcaagatgtt cggcgggagc gtccccaacc tcttcgcaac ggccgcgcag
      301 tcaccggccg ccgtcggcgc gatgctctcc ttcttcgcct cgctcggcga gggctcgctc
      361 ggcgccaagg caggagagca gatcgccatc gccatcgcgc agtcgaatgg ctgcggctac
      421 tgcctctcgg cccacaccgc catcggctcg atggtcggcc tcgcccccgc ggcgctggct
      481 gccgcacgcc gggcccaggg gaccgacccg aagacccagg cgctcctgac cctcgcgctc
      541 gccgtcaatc agacccgggg ccacatcgac gatgcaaccc tggccgcggc gcgcctggcg
      601 ggcttgggcg acgccgaaat cgtcgaactc gtggcgcacg tcgcgctcaa cgtgttcacc
      661 aactacctga acaacgtggc ggaaacgaag atcgacttcc ccgaagtctc gctcttcgcc
      721 gccgcctgat cgagggttcc catgaccagc acgatccgac cgccgttcac gctggacacc
      781 gcgcttgcca aggtgcaggc cgccgaggac gcgtggaaca gccgcgaccc cgaccgggtc
      841 gtgctggcct atcccgagga ctccgactgg cgcaaccgca gcgaattcct ccgcggccgg
      901 gaagcgatcc acgccttcct gcaatgccag tgggcccggg agcgtatccg gtgagcctcg
      961 ttgtcgcggg actctggcgc tacccgatca agagtctggc cggcgagccg atcgacgcgg
     1021 ccgagctcac gccgaccgga atcccggggg accgggtggt ccacgtgcgc ggccctgagg
     1081 gcgtgcggac ctcgcgacga cactaccggc tgctcggact gcgcgggacg ctcgatgcgt
     1141 ccggcaggcc cctgatcaac ggcctgccgt gggacggcga ggaggcactg gctctggtgc
     1201 gccgcgccgc cggcgaggat gcctggctcg aggcgtacga tggcgccgag cggttcgacg
     1261 tcctgcccct gctcgtcgcc accgacggcg cggtggccgc gttcggccgc gacatccgcc
     1321 ggctgcggcc caacatcctg atcggtggtg tggacgggct cgccgaacgg tcgtgggagg
     1381 gagccacgtt gcggatcggc ggcgcggtga tcctgctcga ttcgctgcgg gtccgctgcc
     1441 ccatgaccac cgtggaccct gacacgctca agcgcgacaa ggaagtgctc cgggatatcg
     1501 gccgtcgctt cggcggccgc ctggccctga acgcggccgt agcccggccg gggatcgtgc
     1561 agcgcgggga tcgcgtccgc tacagcgcgg caggactcca cgtcgacgag gcgcgaagat
     1621 aggcgccgac ccgtacgaac cccgtgttac gacttctgag cttcttcaag tggcgcggga
     1681 ttgcgctcgg cggaccgaca tcgcgatcgg caagcccacg aacgggatat gcccgaacca
     1741 ttgcacccac cagcggtacg aaatcctcgg cgggcggtgc gtgagggcgg gaatgaccac
     1801 cagggacatc accatccaga tggccgggcc gtacacggcc gccactttgt agacaccgcc
     1861 gggggaggcc agcacgccgc ggatacgggc cgaccgggac acggcgtaca cgaagatcgc
     1921 cgaccacgtg gtggccacac agaagtgcat cgcgaggccg aggaggaccg tcggggtccc
     1981 gccgctcagg gccccgctgc cgatgagaac cgccgccacg ccctgccaga gacgcgggac
     2041 cgtggacccg tacagcacga ccacgatgct cgagaagagc ccgtcgacga gcgcggtcgt
     2101 aacgccggcg cgcagcacca cgctgagggc gctccgcgga gcgcgacgga tgtcggccgt
     2161 gctcacgcgc gcgcgacctt cgccaccgtg tcccaggtcc gggtcgtgag cgccttgccg
     2221 aacgtcttct cgatgagcgt catgaatacc ggctgccccc tggagtggcg aacgtaggcg
     2281 ctgaggactc cccggtcatg cggggagaga gaacggtctt cacagccggc cggctcgaag
     2341 gcccgcttca ggtcgggcat tttcgcgttc atcggcatga ccccccggag aaacgcggca
     2401 aagcgtggca tgggacgtcc cggccggtca gggtacagtg aaaatcatac gcgtgcccgt
     2461 cgtcggcaat cgtgctcccg cctcgcgggg gcgctcagcg ctcctggtcc cgcaacaacg
     2521 gttgcctggt gtattgcgtg gccaacgagg tgagcggcgc ttcgagcgct gcgccctctc
     2581 gccggcgggc ggcgacacgg acatacccga gataccggcc agcgtcggcc tccacggccg
     2641 tgatctcgac ccggttgtcg ggcgcgatcg cccgctgcca atcgagccag cgccgccacc
     2701 cctcgggcat gctgtcggcc agcgcgacgt ccacgatgcc cgtccgttcc cagtgtcgcc
     2761 gccaccagtc tgcggagtgc aggcaccaca actccggagt ccaccactcc cggaggtgct
     2821 cgggaatcgg accgtcgatc tcctgcatga gtccggctcc ggcgatcgcc agctgggcgc
     2881 ccggccgcag gaagcgcgcg agggtgccca gatacaagtc gtccgtgccg aaatagggat
     2941 agccgtcgat gcacacggcg gcatcgaaga actccgcggc gaagggcagc gaccgggcgt
     3001 ccgcatggat ggggaatacg ccatcctcca caccggcatc gcgaatccgc tgcaggttct
     3061 gggacacgtc gaaccacaga tcggtggccc agacctgcac cccgaattcc ctgtgcagga
     3121 agatggatga cgcggctctc ccgcatccga ggtccagtac cttcatgccg ggccggagat
     3181 cgagcgccgt ggcaagccat tcgacgagcc acagggcgtt ggccccgccg ctgacactgg
     3241 cccggaccca ctcgtggtgg tagctcgccg agcggggaaa ccggtcgttg gtcagaccat
     3301 cagtcgccgc catggacctc gataattcta agcctttctt caccgcaatg tgtcccgtct
     3361 ccacacgtct cgctgccgat cttcctgacg accggccggg gtcatcgccg tctttcagcg
     3421 agcgtcacac cggccggacg ctgcgaggag gaggattcca tgagaggatt catcggcgac
     3481 atcgagaacc ggaccgaggc gaatcaggac ttccggcgcg tactctacac gggcccgcac
     3541 atgcagctgg tcctcat
//
LOCUS       DBNGZW010000571         1439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_257564, whole genome shotgun sequence.
ACCESSION   DBNGZW010000571 DBNGZW010000000
VERSION     DBNGZW010000571.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1439
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_257564"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            91..1038
                     /locus_tag="AC16JC_13540"
     CDS             91..1038
                     /locus_tag="AC16JC_13540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016866178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR/zinc-dependent alcohol dehydrogenase-like
                     family protein"
                     /protein_id="HIR5165766.1"
                     /translation="MKGVWLEDRRLRLRDDLPVPEPPPGEALVRVLAAGICNTDLELV
                     RGYYPYTGVLGHEFAGEVVSDGALAGKRVVGEINAVCCDCWACRAGRRSHCERRTVLG
                     IVNRHGAFAEYLTLPVENLHVIPDRVSTEVAVFTEPLAAALEIQEQVAIDPAMRVLVV
                     GDGKLGQLVAQTLALTGCELFVSGRHDDKLELLRRRGIATMAPDAIPAEAFDVAVECT
                     GNPEGFAVAQRALRPRGTLVMKSTYADRLTLDASAVVVNELTLIGSRCGPFPKALALL
                     AVVAVEVLPLVTARYPLERALDAFEHAQRPGTLKVLIGR"
     gene            complement(1042..>1439)
                     /locus_tag="AC16JC_13545"
     CDS             complement(1042..>1439)
                     /locus_tag="AC16JC_13545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HIR5165767.1"
                     /translation="HWFNLRDAGRRNSWLLPAAVAGLVILAIVTAPGRPGGTAADGAA
                     SVSFADIRVVIARRCAPCHSSSPTYPGMAAAPAGVMLDTPEQIQARAARIAERAVVQQ
                     TMPLGNVTGITGEERALLGRWVRAGAPLR"
BASE COUNT          211 a          480 c          529 g          219 t
ORIGIN      
        1 ggatgtgtcg ccgatcgacg acatccgctc gagcgcgaca taccggcagc gggtgatggc
       61 gcgcgtgttc tacgatgcgg tgcgcgggtc atgaaaggcg tctggctcga agatcgccgg
      121 ttgcggctgc gggacgatct ccctgtaccg gagcctcccc ccggggaagc attggtccgg
      181 gtgctggccg ccgggatctg caataccgac ctggaactgg tgcgcggcta ctacccctac
      241 accggcgtgc tgggacacga gttcgcgggt gaagtcgtgt cggacggtgc gctcgccggc
      301 aagcgggtgg tgggcgagat caacgcggtg tgctgcgact gctgggcgtg ccgggcgggc
      361 cgtcgcagcc actgcgagcg gcgcacggtg ctgggcatcg tcaatcgcca cggcgccttc
      421 gccgagtatc tgaccttgcc ggtcgagaac ctgcacgtga ttcccgacag ggtctcgacg
      481 gaggtcgcgg tcttcaccga accgctcgcc gcggcactcg aaatccagga gcaagtggcc
      541 atcgatccgg ccatgcgggt tctggtcgtc ggggacggga agttggggca gctcgtcgcc
      601 caaaccctgg ccctcaccgg atgtgagctg ttcgtgtccg gccgtcacga cgacaagctc
      661 gagctgctgc gacgccgcgg gatcgcgacg atggcgccgg acgccatccc ggccgaggcg
      721 ttcgacgtag cggtcgagtg caccgggaat cccgagggat tcgccgtggc gcagcgcgcg
      781 ctgcggccac gcggcacgct ggtaatgaag agcacctacg ccgacaggct tacgctcgac
      841 gcctcggcgg tcgtggtgaa cgagctgacg ctgatcggct cgcgctgcgg tccctttccc
      901 aaggcgctcg ccctgctcgc tgtggtggcg gtggaggtgt tgccgctcgt gacggctcgc
      961 tatccgctgg aacgggcgct cgacgcgttc gaacacgcgc aacgaccggg gacgctgaag
     1021 gtcctgatcg gccgatgaga ctcagcggag cggcgcgccc gcgcgcaccc agcgcccgag
     1081 cagcgcccgc tcctcgcccg tgatccccgt gacgttgccg agcggcatcg tctgctgcac
     1141 caccgcccgc tcggcgatgc gtgcggcgcg ggcctggatc tgctccgggg tatcgagcat
     1201 gaccccggcc ggggcggccg ccatcccggg ataggtcggt gacgaggaat ggcagggggc
     1261 gcagcgccgt gcgatcacca cccggatgtc ggcgaatgaa acagaggccg ccccgtccgc
     1321 cgcggtgcca cccgggcgac ctggtgccgt gacgatcgcc aggatcacga ggccggccac
     1381 cgccgccggg aggagccagg agttgcgccg tccggcgtcg cgcaggttga accagtggc
//
LOCUS       DBNGZW010000572         3250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1348430, whole genome shotgun sequence.
ACCESSION   DBNGZW010000572 DBNGZW010000000
VERSION     DBNGZW010000572.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3250
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1348430"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..703
                     /locus_tag="AC16JC_13550"
     CDS             <1..703
                     /locus_tag="AC16JC_13550"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR5165768.1"
                     /translation="QYLIAPTVTWPVTAATLVSLGLVLKDAESTPDSTRLIGVLRPYG
                     VGSFQQVGVTGELRVDTRDLPAWATRGALLAISGSAYPATLDVTRAFGEVTAQAATYL
                     TIPHGPTLALRAAGSRAFGPYPFHEAAFVGGGATVRGYAEHRFAGDASLAGNVELRAR
                     VGQVYALLPIEFGVFGLADAGRVFRSGETSDVWHGAAGGGLWFALLNRVNTLTVAVAR
                     SPESTGIYVRAGFAY"
     gene            708..>3250
                     /gene="ppk1"
                     /locus_tag="AC16JC_13555"
     CDS             708..>3250
                     /gene="ppk1"
                     /locus_tag="AC16JC_13555"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR5165769.1"
                     /translation="MSAPPVPEWRGEVPSLDLLNRMVLEPLPLGLLAASVERTFHRDL
                     YFDAPDWTLRRRGVSCRFRVRLDDRRFLTVRSLGRTEDAAMVVIPQTFEAEVEELTGE
                     QAVAGDSEPARRLRALIDPSRLVPRLEFETDRRLRRSRPGWIARARFEFLYDVVTVRS
                     QDLSQTLQEVTLRRVAAGRPAFEAVVNAVRDTYGLRPLLVGKFERAEKLLQQLEAETV
                     ARSTRGQREIVLIALEESTIALRYDRGTLSLPAAGGSGEEGVRHLLRDTFGSGDGQLR
                     FLGLAPTVGDRAQLEVWLSRRVSAHGAAGGACRWLPLAEVLARVGSPALREPRTLAAL
                     AVAARADVFPDHAPVPAPTPRRSLVEVPEATPLADDVPEPAAEHLLNEDLSTLAFNAR
                     VLELAEDPQVPLLARVRFLSIFSANLDEFFMVRMGALARAAAAGSTKRSDDGLTPRGQ
                     LEAIGLRLRPLIERQERCWVQSCAPALAREGIRVLRWAELNDAQRDAMRRYFTDRVFA
                     TLTPQAITRAPGHPFPVLPNARVCLAVVVRDPRSGAQHFACVKTPDGLARFVPLEGEQ
                     FVPLEDVIRENLETLYPGRRVEAAHAFRITRNADLELPEVGAASLLQVVDEETKRRPY
                     GAVVRIEVDQAMPQAIRDLLLRELQFDDAGEVSTLGPGDLYEAAPYVDLGALRELAGL
                     PRAELQYPPFRGRTPLPADRSIFELLRRGDVLVHHPYDSFEATVERFFAEAADDPAVL
                     AIKLTLYRAGGRSGIVDALLRAAAAGKDVFVFVELKARFDEERNVDWARRLEDAGIHV
                     VYGLVKLKTHCKTALVVRREDDVVRRYVHIGTGNYNATTAGLYTDLGLLS"
BASE COUNT          436 a         1144 c         1154 g          516 t
ORIGIN      
        1 gcaatatctg atcgccccga cggtcacgtg gcccgtgacc gccgcgacgc tcgtgtcgct
       61 cggtctggtg ctgaaggacg cggaatcgac gccggattcc acccgcctga tcggggtcct
      121 gcggccctac ggggtcggat cgtttcagca ggtcggagtc acgggggagc tgcgcgttga
      181 tacgcgggac ctcccggcct gggcgacgcg cggcgccctg ctcgcgatct cgggcagcgc
      241 ctatccggcg acgctcgacg tcacccgggc gttcggcgag gtcacggccc aggccgcgac
      301 ctatctgacc atcccgcatg gaccgaccct cgcgctgcgc gccgcgggct cgcgggcgtt
      361 cggcccctat cccttccacg aagcggcgtt cgtcggtggc ggcgcgaccg tgcgcgggta
      421 cgccgagcat cgcttcgcgg gtgacgcgtc gctggccggg aacgtggagt tgcgcgcgcg
      481 tgtgggtcag gtctacgcgc tgttgccgat cgaattcggc gtgttcgggc tcgccgacgc
      541 cgggcgagtg tttcggtccg gcgagacgtc ggacgtgtgg cacggcgccg ccggtggcgg
      601 actctggttc gccctgctga atcgcgtgaa taccctgacg gttgccgtgg cgcgctcgcc
      661 cgagagcacg ggcatctacg tgcgcgccgg cttcgcctac tagtccgatg agcgcgccgc
      721 cggttcccga atggcgcggg gaagtcccca gcctcgatct cctgaaccgg atggtgctcg
      781 agcccctgcc cctggggctg ctcgccgcat cggtcgaacg gaccttccat cgcgatctgt
      841 acttcgatgc accggattgg acgctgcggc gccggggcgt gtcgtgccgg ttccgcgtgc
      901 ggctcgatga ccgccggttc ctgacggtgc ggagcctggg gcggacggag gacgccgcga
      961 tggtcgtcat cccgcagaca ttcgaggccg aggtcgagga gctcacgggc gaacaggccg
     1021 tcgcgggtga ctccgagccg gcgcgacgcc tccgcgccct gatcgatccc tcccggctcg
     1081 tcccgcggct cgagttcgag accgaccgcc ggctgcggcg ctcgcgcccg ggatggatcg
     1141 cccgcgcccg attcgagttc ctctacgacg tggtaacggt acgcagccag gatctgtcgc
     1201 agacgctgca ggaagtgacg ctgcgacgtg tcgccgccgg ccggccggcc ttcgaggcgg
     1261 tggtaaacgc cgtgcgcgac acctacggcc tgcggcccct gttggtcggc aagttcgagc
     1321 gggccgagaa gctcctgcag cagctcgagg cggagaccgt ggcgcgatcc acgcgcggcc
     1381 agcgcgagat cgtcttgatc gccctcgagg aatccacgat cgcgctgcgc tatgaccgtg
     1441 gcaccctttc gctgcccgcc gccggcggca gcggggagga gggcgttcgc cacctgctgc
     1501 gcgacacctt cgggagtggc gacgggcaat tgcgcttcct cgggttggct ccgacggtag
     1561 gggacagagc gcagctcgag gtatggctgt cccgccgggt gagcgcgcat ggcgcggcgg
     1621 gcggcgcatg ccggtggctg ccgctcgccg aggtcctggc acgcgtcggc tcgccggcgc
     1681 tgcgggagcc ccggacgctc gccgcactgg cggtcgccgc gcgggccgac gtgttcccgg
     1741 accatgcgcc ggtgccggcg ccgacgccgc ggcggagcct cgtcgaggtc cccgaagcca
     1801 ccccgctggc ggacgatgtc ccggaaccgg cagcggagca tctgctgaac gaggacctat
     1861 ccaccctcgc cttcaacgcc cgggtcctcg agctggccga agacccccag gtgcccttgc
     1921 tggcgcgcgt gcggtttctc tcgatcttca gcgcgaacct ggacgagttc ttcatggtgc
     1981 gcatgggagc gctcgcccga gcagccgccg ccgggtccac caagcgcagc gacgacgggt
     2041 tgaccccccg gggacagctg gaagcgatcg ggctgcggct ccgcccgctg atcgagcggc
     2101 aggagcggtg ctgggtccag agctgcgccc cggcactggc ccgggaaggc atccgggtgc
     2161 tgcggtgggc ggaattgaac gacgcgcaac gggacgcgat gcgccgctat ttcaccgatc
     2221 gcgtgttcgc caccctcact ccgcaggcca tcacccgggc gccggggcat ccctttccgg
     2281 tgctgcccaa cgcccgcgtc tgtctcgcgg tggtcgtacg cgatccgcgc agcggcgccc
     2341 agcacttcgc ttgcgtgaag acccctgacg gtctcgcccg gttcgtcccc ctggagggcg
     2401 agcagttcgt gcccctcgag gacgtgatac gggagaacct cgagacgttg tatcccggcc
     2461 gccgggtgga ggccgcccac gcgttccgca tcacccgcaa cgccgatctc gagctcccgg
     2521 aggttggggc cgcgagcctg ctgcaggtcg tggacgagga gaccaagcga cgcccctatg
     2581 gggcggtcgt gcgtatcgaa gtggatcagg ccatgcctca ggccatccgc gacctgctgc
     2641 tgcgcgagct gcagttcgac gacgccggtg aggtcagcac gctcggcccc ggtgatttgt
     2701 acgaggcggc gccgtacgtc gatctcggcg cgctgcggga gctggcggga ttgccgcgcg
     2761 ccgagctgca gtatccgcca tttcgcggcc gcactcccct gcccgccgat cggtcgatct
     2821 tcgagctgct ccgccggggc gacgtgctcg tgcaccaccc ctacgactcg ttcgaagcga
     2881 cggtcgagcg cttcttcgcg gaagcggccg acgaccccgc cgtcctggcg atcaagctca
     2941 cgctgtatcg cgccggtgga cgctcgggaa tcgtcgatgc gctgctgcgc gccgccgcag
     3001 cgggcaagga cgtcttcgtc ttcgtcgagc tcaaagcacg cttcgacgag gagcgcaacg
     3061 tcgattgggc ccgccggctg gaggacgccg gcatccacgt cgtatacggc ctggtgaaat
     3121 tgaaaaccca ctgcaagacg gccctggtgg tgcgtcgcga ggacgatgtc gtccggcgct
     3181 acgtgcacat cggcaccggc aactacaacg ccacgaccgc cggcctctac accgacctcg
     3241 gactgctgtc
//
LOCUS       DBNGZW010000573         2052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_324907, whole genome shotgun sequence.
ACCESSION   DBNGZW010000573 DBNGZW010000000
VERSION     DBNGZW010000573.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2052
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_324907"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..334)
                     /locus_tag="AC16JC_13560"
     CDS             complement(23..334)
                     /locus_tag="AC16JC_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HIR5165770.1"
                     /translation="MPAYVVVQIEIDDPVGYERYKQLAPPSIAAYGGRYVVRAGPSEP
                     LEGNWQPPRLVMLEFPSTERARAWWGSPEYAPAKALRQRCARTEMLLIEGYTPPLPTP
                     E"
     gene            complement(568..>2052)
                     /locus_tag="AC16JC_13565"
     CDS             complement(568..>2052)
                     /locus_tag="AC16JC_13565"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5165771.1"
                     /translation="IVWTSDPGGQFVEPQPAWEAYTGQSWEEYRGFGWMEALHPDDRD
                     AFRTAWFDARDRRPVYEAVVRVWHRDPGRYRHFAARAAAVRGADGAVREWIGTLTDIE
                     DQRTAEQRLRQAERLESVGRLAGGVAHEANNQMTVVLGSAAFLLRQRPDERAREDIEH
                     IRRAAQRTAAITQQLLAFSRRQILQPQVVDLNATIRNLEPVLQRALGESSRVMLRLAA
                     DLAAVKADPGQLDQVLLNLTFNARDAMPHGGDLTVETANVTLSRQYVAAKGLQSMAPG
                     PYAMLMVSDTGEGMSQETLSHIFEPFFTTKGVGQGTGLGLSTVYGIVKQSGGFVWVYS
                     EPGHGTAFKIYLPALTSATPDHPLVPPPTVQGGAEVILVAEDDELVRGVLARSLREYG
                     YTVLEAQDGADALEIALKGSRPPGLVIADVVMPRLNGRELSTALRRRWPELSVLFISG
                     YTDLDSVTRGLVEEGCDFLQKPIETEVLARKVRAMLEATKGGGR"
BASE COUNT          305 a          740 c          689 g          318 t
ORIGIN      
        1 agcagcatga acttcacgca ggttactccg gggtaggaag aggtggggtg tacccttcga
       61 tcagcagcat ctccgtccgc gcgcatcgct ggcgcaaggc cttggccggg gcgtattcgg
      121 gggaccccca ccaggcgcgt gcccgctcgg ttgaggggaa ctccagcatg acgaggcgcg
      181 gcggttgcca gttgccctcc agcggctcgc tcgggcccgc gcgaaccacg tatcggcctc
      241 cgtaggcggc aatcgacggc ggggcgagct gcttgtagcg ctcgtacccg accggatcgt
      301 cgatctcgat ctggaccacg acataggcgg gcatgactct ggcacctccg catccagggg
      361 cgggtcagcg cgtcgcggcc gacggctctc ccggggacgc cgccgaccgg cttccgtgca
      421 ccattaccag cagcaccccg gccgcataga ccagcaggtc cggacgccac tcgccgcgcg
      481 tcgcgtgcag gatcgcggcg gggacgatgg cagcgaggag cagaacatca cgcgggcgtc
      541 ccggagctgc gcttcgcgcg tcatggctca gcgcccccca cccttcgtcg cctccagcat
      601 tgcgcggacc ttcctcgcca gcacttcggt ctcgatcggc ttctggagga agtcgcagcc
      661 ctcttccacc agaccccggg tcacggaatc gagatcggtg tagccggata tgaacaggac
      721 cgagagctcc ggccaccggc ggcggagcgc cgtcgagagc tcccggccgt tcagccgggg
      781 catgaccaca tccgcgatca ccaggccggg agggcgcgat cccttcaggg cgatctccag
      841 cgcatccgcg ccgtcctgag cctccagtac cgtgtacccg tattcccgga gcgagcgcgc
      901 caggacgccc cgcaccagct catcatcttc cgccaccagg atgacctcgg ccccgccttg
      961 caccgtcggc ggtggcacca gcggatgatc gggcgtggcc gaggtgagcg cgggcaggta
     1021 gatcttgaac gcggtcccat ggccgggctc gctgtacacc cagacgaagc ccccgctctg
     1081 cttgacgatg ccgtacacgg tggacaggcc gagccccgtg ccttgaccca cgcccttggt
     1141 ggtgaagaag ggctcgaaga tgtggctcag cgtttcctgg ctcatgccct cgccggtgtc
     1201 gctgaccatg agcatcgcat acggcccggg cgccatcgac tggaggccct tggccgcgac
     1261 gtactggcgg ctcaacgtca cgttggcggt ctcgacggtc aggtcgcccc cgtgcggcat
     1321 cgcgtccctg gcgttgaacg tcaagttcag cagcacctgg tcgagctggc cgggatcggc
     1381 cttcacggcc gccaggtcgg ccgccagcct gagcatcacg cgcgaggatt ctcccagtgc
     1441 ccgctggagc accggctcca ggttgcggat cgtggcgttg agatcgacga cctggggctg
     1501 gaggatctgt cgccggctga aggcgagcag ctgctgggtg atcgccgccg tgcgctgcgc
     1561 cgcgcgccgg atgtgctcga tgtcctcccg ggcgcgctcg tcgggccgtt gccgcaagag
     1621 gaacgccgcc gaccccagta cgacggtcat ctgattgttg gcctcgtggg ccacgccccc
     1681 ggcaagtcgt ccgaccgact ccaggcgttc cgcctgccgc agccgctgct ccgccgtccg
     1741 ctgatcctcg atgtcggtga gggtcccgat ccactcccgc acggcgccgt cggcgccccg
     1801 cacggcggcg gcccgggccg cgaagtgccg gtagcgacca gggtcccgat gccagacccg
     1861 gacgaccgcc tcgtagaccg ggcgccgatc gcgggcgtcg aaccacgccg tcctgaacgc
     1921 atcgcgatcg tcggggtgca gggcctccat ccatccgaag ccgcggtact cctcccagga
     1981 ctgcccggtg tatgcctccc acgccggctg cggctcgaca aattgtccgc cggggtcgga
     2041 ggtccagacg at
//
LOCUS       DBNGZW010000574         4639 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1403808, whole genome shotgun sequence.
ACCESSION   DBNGZW010000574 DBNGZW010000000
VERSION     DBNGZW010000574.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4639)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4639)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4639
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1403808"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="AC16JC_13570"
     CDS             <1..909
                     /locus_tag="AC16JC_13570"
                     /inference="COORDINATES: protein motif:HMM:NF017183.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative Ig domain-containing protein"
                     /protein_id="HIR5165772.1"
                     /translation="RYDPLGTLKGAVSLDDLKFFALHMGLAFSPAGDLYITNGLDAIY
                     RIVDGVPQLAFRGTPTLGAPVFDRDGFLYVANPGLGRVVLLDPDFHVAQDPFARSYQG
                     GSSALAFLRDATGAMTSRLVTGSVGLGALASDLGVIAELNPNGVRAGGFRVGVDLLRV
                     ANATLPDGVVGADYGETLQLVAAPAPAVWSVSAGALPPGLTLSPDGRLTGIPTRSGAF
                     SFAVRAAAGTQLAYKAFTVTVTVPAVTVEAAAADLLGGAPLALTRRHFLDLQGNRNGN
                     FDIGDFRAFLRANGQLPAAAAGEEQP"
     gene            complement(914..2557)
                     /locus_tag="AC16JC_13575"
     CDS             complement(914..2557)
                     /locus_tag="AC16JC_13575"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF045305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HIR5165773.1"
                     /translation="MIPSQFQPPLPLDQIILKDGAEGAGDRMELDVLFVGAGPAGLAG
                     AIELARLAKTDPGVGELNIGVLEKAESLGEHSLSGAAVNPRALRELFPNLKDSDFPFR
                     GPVTEEAVYFLTSGGQVRFPVTPPTMKNHGCYLASICEIVRWLGHKAEELGVNVFTGF
                     PAAALLTEGNRVAGVRTQATGLGRDGQQLASYQAPNDLTAKVTALSEGTRGMLSQAWL
                     EWQRVGSQNPQIFALGVKEIWETKRPLDRVIHTMGWPLPSDAFGGTFCYPLEPNLLAL
                     GVVVGLDYHDAGLDVHVLLQRLKQHPLFRQYLEGGEMVEWGAKTIPEGGYYALPARRS
                     GDGVVLLGDAAGFVDVPSLKGIHYAIQSGIYAARAIFAALKAGDTSAARLAEYDRVVD
                     ASYIASDLYKTRNMRLAFKDGFYAGGFKAGLMTLSGGRFPGGRIAMHRDADVARRADG
                     AEAFTPDGKLTFSKVDAVFKAGNATRDTIPTHLVVGKDVTPEAARFYTHLCPAGVYEE
                     VDGKLRVNAPNCVDCKATDVVGPRWTPREGGSGPKYKRM"
     gene            complement(2712..3686)
                     /locus_tag="AC16JC_13580"
     CDS             complement(2712..3686)
                     /locus_tag="AC16JC_13580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR5165774.1"
                     /translation="MSHVLAVLEQRDGTLRKVSHEVVTGARRLADALGGEVHALVLGA
                     GAISGTDAAGRFGADKVITATNAGFAHYAPEGYARAAAERAKAGGYGAVVLAASAQGK
                     DLAPRVAARLGVPLAADVTDLAVEGGAVVVTRPVYAGKALLKVKVTAAPAVVSLRPNV
                     FTPVERPKAGAAETVVVAGDAGRVVVKGIKTAAGGALDVAEAPIVVSGGRGLKEPANF
                     KLLEELAQAFGGKAAVGASRAVVDAGWRPHGDQVGQTGKTVSPSLYVAVGISGAIQHL
                     AGMRTAKVIVAINKDKDAPIFKVADYGIVGDLFEIVPRLTEEIRRLNG"
     gene            complement(3683..4435)
                     /locus_tag="AC16JC_13585"
     CDS             complement(3683..4435)
                     /locus_tag="AC16JC_13585"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR5165775.1"
                     /translation="MKIAVCIKRVADSETRVKIAADGKSLDDAGVKFILNPYDEFAVE
                     EALRRKEQAGAGEVVVIACGPQTSQETIRTALAMGADRGVLLEAASVPADGLAVARAL
                     AAELKGGGYDLILFGKVAIDDSNQQVGPMVAELLDLPCVTCAAHLDLAGGKGTAEREV
                     EGGMEEVEFPLPAVITADKGLNEPRYPALKGIMAAKKKPLEVKPAALGASGVEILAMT
                     LPQERKEGRIVGEGAAAVPALLQLLREEAKVL"
BASE COUNT          681 a         1708 c         1495 g          755 t
ORIGIN      
        1 cgctatgacc cgctcggaac cttgaagggc gccgtcagtc tcgacgacct gaagttcttc
       61 gccctgcaca tgggactcgc cttctccccc gccggtgacc tctacatcac gaacggcctc
      121 gacgcgattt accggatcgt ggacggcgtc ccgcagctgg cgttccgggg gacccccacc
      181 ctcggcgccc ccgtattcga ccgggacggg ttcctgtacg tcgcgaaccc gggcctggga
      241 cgtgtggtcc tgctcgatcc cgactttcac gtcgcccagg atccgttcgc ccgcagctac
      301 cagggcggat cgagcgcgct cgccttcctc agggacgcga ccggcgcgat gacgtcgcgg
      361 ctggtcacgg gcagcgtggg actcggggcc ctggcctccg acctcggcgt gatcgccgag
      421 ctgaacccca acggggtgcg cgcgggcgga ttccgcgtcg gcgtggatct gcttcgcgtg
      481 gccaatgcga cactcccgga cggcgtggtc ggcgcggatt acggcgagac gttgcagctc
      541 gtcgcggcgc ccgccccggc ggtctggagc gtgtccgccg gagcgctgcc gccggggttg
      601 accctctcgc ccgacggccg cctcaccgga atccccacgc ggagcggcgc gttcagcttc
      661 gccgtccgcg ccgctgccgg cacccagctg gcgtacaagg ccttcaccgt gaccgtgacc
      721 gtgccggccg tgaccgtcga agccgcggcg gcggacctgc tcggcggagc accgctcgcc
      781 ctgacgcggc gccacttcct cgatctgcag ggcaaccgga acgggaactt cgacattggc
      841 gacttccgcg cgttcctgcg cgcgaacggc cagctccccg cggcggcggc gggagaggag
      901 cagccatgag acctcacatc cgcttgtact tcggcccgct gccgccctcc cgcggcgtcc
      961 agcgtggccc cacgacgtcg gtcgccttgc agtccacgca gttcggcgcg tttacgcgca
     1021 gcttgccgtc cacctcttcg tagaccccgg cgggacagag gtgcgtgtag aaacgcgccg
     1081 cctcgggcgt cacgtccttc ccgacgacca gatgggtcgg gatggtgtcg cgcgtagcgt
     1141 tcccggcctt gaagaccgca tccaccttgc tgaacgtcag tttgccgtcc ggcgtgaacg
     1201 cctcggcacc atcggcgcgg cgcgccacgt cggcgtcgcg gtgcatcgcg atgcgaccgc
     1261 ccgggaaccg gccaccgctg agcgtcatca gcccggcctt gaatcccccc gcgtagaagc
     1321 cgtccttgaa tgccagccgc atgttgcgcg tcttgtagag gtcgctcgcg atgtaggacg
     1381 cgtccaccac ccggtcgtat tccgcgagcc gcgcggctga cgtgtcgccc gctttgagcg
     1441 cggcgaagat cgcgcgcgcg gcgtagatcc ccgactggat ggcatagtgg atccccttga
     1501 gcgacggcac gtccacgaac cccgccgcgt cacccagcag caccacccca tcccctgagc
     1561 ggcgcgccgg gagcgcgtag tagcccccct cggggatcgt cttggcgccc cactccacca
     1621 tctcgccgcc ctcgagatac tgccggaaca gcggatgctg cttcagccgc tgcagcagca
     1681 cgtgcacgtc gagcccggca tcgtgatagt ccaggccgac cacgacgccc agcgccagga
     1741 gattcggctc gagtggatag cagaacgtgc cgccgaacgc gtcggacggt agcggccagc
     1801 ccatcgtgtg aatcacccgg tcgagcggcc gtttcgtttc ccagatctct ttcacaccga
     1861 gcgcgaagat ctgcggattc tgcgagccga cacgctgcca ctcgagccat gcctgcgaca
     1921 gcatgccgcg cgtgccttcg gagagtgccg tgaccttcgc ggtgaggtcg ttgggtgcct
     1981 ggtaggaagc gagctgctgc ccgtcacgcc ccagccccgt ggcctgggtg cgtacccccg
     2041 ctacgcggtt gccctcggtg agcaacgccg ccgccgggaa gccggtgaag acgttgaccc
     2101 ccagctcttc cgccttgtgc cccagccagc gcacgatctc acagatcgag gcgaggtagc
     2161 agccgtggtt cttcatggtc ggcggcgtca cggggaaccg cacctgaccg ccggaggtga
     2221 ggaagtagac ggcttcctcg gtcaccgggc cacggaacgg gaaatcgctg tccttcagat
     2281 tggggaacag ctcgcgcagc gcgcgcgggt tgaccgccgc gcccgagagc gaatgctctc
     2341 ccagcgactc ggccttctcg aggaccccga tgttcagctc gcccacgccg ggatcggtct
     2401 tggcgagccg cgccagctcg atcgcgcccg cgagccctgc cggccccgcc ccgacgaaca
     2461 gcacgtcgag ctccatgcga tcgccggcgc cctcggcgcc atcctttaga atgatctgat
     2521 cgaggggcag cggcggctga aattgagatg ggatcatgac aattgcggat tgtggattgc
     2581 ggattgcgga ttggggcatt tcaattgcgg attgtgcaat gcggattgcg gattgcggac
     2641 tgtatgcgtc acgtcgccag gcaagcgacc ggctcatatc caatccgcaa tccacaatcc
     2701 gcaatccgca attacccatt gagtctccga atctcctcgg tgagcctcgg cacgatttcg
     2761 aacaggtctc ccacgatccc gtaatccgcc accttgaaga tcggggcgtc cttgtccttg
     2821 ttgatcgcca cgatgacctt cgcggtgcgc atccccgcca ggtgctggat cgcgcccgag
     2881 atgccgacgg cgacgtacag cgacgggctc accgtcttcc ccgtctgccc cacctggtcg
     2941 ccgtgcggcc gccagcccgc gtccaccacc gcgcgggacg cacccaccgc cgccttgccc
     3001 ccgaacgcct gcgccagctc ttcgagcagc ttgaaattcg cgggctcctt caggccgcgc
     3061 ccgccggaca ccacgatcgg cgcctcggcg acgtcgagcg cgccgcccgc ggcggtcttg
     3121 atgcccttca ccacgacgcg gccggcgtct cccgcgacca cgaccgtctc cgccgctccc
     3181 gccttgggcc gctcgaccgg cgtgaacacg ttgggccgca gcgacacgac cgccggggcc
     3241 gcggtcacct tcaccttgag cagcgccttg ccagcgtaga ccggccgcgt gacgacgacc
     3301 gccccgcctt cgacggcgag atcggtgacg tcggccgcga gcggcacacc gagcctcgcg
     3361 gcgacgcgcg gcgcgaggtc ctttccctgc gcgctcgcgg cgagcacgac ggcgccgtag
     3421 ccgcccgcct tggcgcgctc ggccgccgct ctcgcgtacc cttcgggcgc gtagtgggca
     3481 aagccggcgt tggtggcggt gatcaccttg tcggccccga atcgaccggc ggcatcggtg
     3541 cccgagatcg ccccggcgcc gaggaccagg gcgtgcacct cgccccccag ggcgtcggcc
     3601 aggcgccggg ccccggtcac cacctcgtgg ctgactttgc gcagcgtccc gtcacgctgc
     3661 tccagtaccg ccagcacgtg gctcatagga ccttggcctc ttcccgcagc agctgcagca
     3721 gcgccggcac cgcggccgcg ccttccccga cgatgcgccc ctctttccgc tcctgaggga
     3781 gcgtcatcgc caggatctcc acaccggacg caccgagggc ggccggtttc acctcgagcg
     3841 gcttcttctt cgccgccatg atgcccttga gcgccgggta gcggggctcg ttcaacccct
     3901 tatccgccgt aatgacggcg ggcaggggga actccacttc ctccatcccc ccttcgacct
     3961 cgcgctcggc cgtgcccttg ccccccgcca gatcgagatg cgcggcgcag gtgacgcagg
     4021 ggaggtcgag cagctccgcc accatcggcc ccacctgttg gttggagtcg tcgatcgcga
     4081 ccttgccgaa gaggatgaga tcgtagccgc cacccttgag ctcggcggcg agcgcccgcg
     4141 ccacggcgag accgtcggcc ggcacactgg cggcttcgag cagcacgcca cggtcggctc
     4201 ccatggcgag ggccgtgcgg atggtctcct gggaagtctg gggaccgcag gcgatcacca
     4261 ccacctcgcc ggcacccgcc tgctccttgc ggcgcagcgc ctcttcgacc gcgaactcgt
     4321 catagggatt gagaatgaac ttgacgccgg cgtcgtcgag cgacttgccg tcggcggcga
     4381 tcttgacgcg cgtttccgag tcggcgacgc gcttgatgca gactgcgatc ttcatgaggc
     4441 aggggccagg tgtcgggtat ccggtttcag gtgtcaggtg caagggagcg cggccagaaa
     4501 agtaacccgg acacctgacg cccggcaccc gacgcctgcc tcaactgaac gcattgatcc
     4561 ccgtaatctc ctgccccagc accagggtgt ggatgtcgtg ggtcccttcg taggtgtaga
     4621 ccgattcgag gttggccag
//
LOCUS       DBNGZW010000575         3367 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1000496, whole genome shotgun sequence.
ACCESSION   DBNGZW010000575 DBNGZW010000000
VERSION     DBNGZW010000575.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3367)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3367)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3367
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1000496"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..815)
                     /locus_tag="AC16JC_13590"
     CDS             complement(<1..815)
                     /locus_tag="AC16JC_13590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5165776.1"
                     /translation="MRTLRLVVALTLPASLFPLPAFAQQKHPITFDDFIGLPVVSDPQ
                     LSSDGKWVAYTVTTPSLKDNRGFGRVWLAEVATGRTRQLTQGPGSDFSPRWSPDGQTL
                     AFISTRQGGPQIWVLGMTGGEARKVTSIDDGVGELYWKPDGRGFLAVVDVKWPAEQEM
                     DRRNGEYPTEARLWTELMWRHWVEFRAGHRQHVFAVELATGKATDLTTVDHDVPTIAT
                     GGDGDVAVSPDGREIAVAMHGDDVVATNTNVDVYLIGDEGRGTGDESKWKNVTA"
     gene            complement(817..1074)
                     /locus_tag="AC16JC_13595"
     CDS             complement(817..1074)
                     /locus_tag="AC16JC_13595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165777.1"
                     /translation="MDLAISRAQLDAVRNAQHLPDVLRRVLDAASLSADEAVLHLTYE
                     EATALQELAAWNVHTDAAGEVTPASKLFDDLVKAIITHPDY"
     gene            1155..2150
                     /locus_tag="AC16JC_13600"
     CDS             1155..2150
                     /locus_tag="AC16JC_13600"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011419066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="HIR5165778.1"
                     /translation="MPRTEFIATGFYVPPRVVSNDDLAKLMDTSDEWITQRTGIKRRH
                     WVEPGTMCSALAAEATRVALARAGLEASDLDCIIFATTSPDHFFPGNGVFLQRLLGLS
                     DIPALDVRDQCTGFIYGLSVADAWIRCGQYKRVLLVGAEIQSPGLDLTTAGRDTAVIF
                     GDGAGVAILGRTDDPKRGVLSTHLFSDGKYAEKLWMDGPGLAHVPWISAAMATDGRAR
                     VHMEGRDVFKHASTKMPESVQLALRTNGATTADVKLFIPHQANQRISEMVQKQLDLRD
                     DQVYNNIQEYGNTTAASIPIALHEALDAGRLARGDLLILTAFGSGFTWGSAAIRW"
     gene            complement(2174..2812)
                     /locus_tag="AC16JC_13605"
     CDS             complement(2174..2812)
                     /locus_tag="AC16JC_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5165779.1"
                     /translation="MAPLRDVDAADQANLIKIAVWFGPAAFIMLSALWYFLAEKQMIP
                     GWVAVVMTILDIPLVVLGVMLIHRGTIHASEGLVNTLYSAGNIPPPPSYPRQDVMIVR
                     GQYREAAEYFRDHLIVEPEDNEARLRLADLVDKHLDDPAEAERLYLEVRRHQPTPGHE
                     IRAANGLIDLYRKLGRRDRLIVELARYADRYRGTPGAEAATRLLQELKADSR"
     gene            2863..3120
                     /locus_tag="AC16JC_13610"
     CDS             2863..3120
                     /locus_tag="AC16JC_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165780.1"
                     /translation="MIIGLLLWGAALGGGFIPTRNFVRRRLRFVDAAQKPSTPVVAGV
                     VAAAAGITAAALPIITVPMGVLFGIGVGAGWASGQKAPPES"
BASE COUNT          537 a         1156 c         1126 g          548 t
ORIGIN      
        1 gccgtgacgt tcttccactt ggactcgtcc ccggtccccc gtccctcgtc cccgatgagg
       61 tagacgtcca cgttggtgtt ggtggcgacg acgtcgtccc cgtgcatggc caccgcgatc
      121 tcgcggccgt cgggagacac cgcgacatcg ccatccccgc ccgtcgcgat cgtcgggacg
      181 tcgtggtcca ccgtggtgag atcggtggcc ttcccggtcg cgagctccac ggcgaacacg
      241 tgctggcgat ggccggcgcg gaactcgacc cagtggcgcc acatgagctc cgtccacagc
      301 cgggcctcag tcgggtactc accgttgcgc cggtccatct cctgctcggc gggccatttc
      361 acgtcgacga ccgcgaggaa cccgcgaccg tcgggcttcc agtacagctc tcccacaccg
      421 tcgtcgatcg acgtcacctt ccgcgcctcg ccccccgtca tgccgagcac ccagatctgc
      481 gggcctccct gccgggtcga gatgaacgcc agcgtctgcc cgtcgggcga ccagcgaggg
      541 gagaaatccg agcccggacc ctgcgtgagc tgccgggtgc gacccgtggc cacctcggcg
      601 agccagacgc gaccgaaccc ccggttgtcc ttcaacgagg gcgtcgtgac ggtgtaggcc
      661 acccatttcc cgtcggacga aagctgcggg tccgatacga cggggagccc gatgaagtcg
      721 tcgaaggtga tgggatgctt ctgctgcgcg aaagcgggaa gggggaagag ggaagcggga
      781 agggtcaatg caacgacgag acgcagggta cgcatgtcag tagtccggat gagtgatgat
      841 ggctttcacg agatcgtcga acagcttcga ggccggcgtc acctcgccgg cggcgtcggt
      901 gtggacgttc caggcggcca gctcctgcaa cgccgtggct tcttcatacg tgaggtgcag
      961 caccgcttcg tcggcggaaa ggctggccgc gtccagtacg cgccgcaaca cgtcggggag
     1021 atgctgcgcg ttgcgcacgg cgtcgagctg tgcgcggctg atggcgaggt ccatgggctc
     1081 ccttgcgaga cgctggttcg ggccaaatct tccctcctcc cgctggaaac gccagacgcg
     1141 cacccaaggc caacatgccc cgcacggaat tcatcgccac cggcttctac gtcccgcccc
     1201 gggtcgtgtc caacgacgac ctggcgaagc tgatggatac ctccgacgag tggatcacgc
     1261 agcgcacggg gatcaagcgg cgccactggg tcgagccggg cacgatgtgc tcggcgctgg
     1321 cggccgaggc gaccagggtg gcgctggcgc gcgccggcct cgaggcgagc gatctggatt
     1381 gcatcatctt cgcgaccacg agcccggacc acttcttccc cggcaacggc gtgttcctgc
     1441 agcgactgct ggggttgagc gacatcccgg cgctcgacgt gcgcgatcag tgcaccgggt
     1501 tcatctacgg cctctcggtc gccgacgcct ggatacgctg cggccagtac aagcgcgtgc
     1561 tgctggtggg cgcggagatt caatccccgg ggctcgacct cacgaccgcg gggcgcgata
     1621 cggccgtgat cttcggggac ggcgcggggg tcgccatcct gggccgcacc gacgacccga
     1681 agcggggcgt gctttcgacc cacctcttca gcgacggcaa gtacgccgag aagctctgga
     1741 tggacgggcc gggtctggcg cacgtgccct ggatcagcgc cgcaatggcg acggatggcc
     1801 gggcccgggt gcacatggaa ggccgggatg tcttcaagca cgccagcacc aagatgcccg
     1861 agagcgtgca gctggcgctg cgcacgaacg gcgcgaccac cgccgacgtg aagctcttca
     1921 ttccgcacca ggcgaaccag cgcatctcgg agatggtcca gaagcagctc gacctgcggg
     1981 atgatcaggt ctacaacaac atccaggagt acggcaacac caccgccgcg tcgattccca
     2041 tcgcgctgca cgaggccctg gacgcgggcc gcctcgcccg cggcgacctg ctgatcctca
     2101 cggcgttcgg ctcgggattc acatggggga gtgcggcgat acgctggtag ggctatcaaa
     2161 tatcggctat cggctatcgg ctatcggctt tcagctcctg gagcaggcgg gtcgcggcct
     2221 ctgcgcctgg tgtcccgcga tagcgatccg cgtagcgcgc caattccacg atgagcctgt
     2281 cgcggcgccc cagcttgcga tacagatcga tcaatccgtt cgcggcgcgt atctcgtgcc
     2341 cgggcgtcgg ctggtgccgc cgcacctcca ggtagagccg ctcggcctct gcgggatcgt
     2401 cgagatgctt gtccaccagg tcggcgagtc gcaggcgtgc ttcgttgtcc tccggctcga
     2461 cgatgaggtg gtcgcggaaa tactcggccg cctcgcggta ctgcccgcgc acgatcatca
     2521 cgtcctgccg tggatagctc ggcggcggcg ggatgttgcc cgccgagtag agcgtgttca
     2581 ccaggccctc ggaggcgtgg atcgttcccc gatggatcag catgaccccc aggacgacga
     2641 gcgggatgtc caggatcgtc atcaccacgg caacccaccc ggggatcatc tgtttctcgg
     2701 ccaggaagta ccacagcgcc gagagcatga tgaacgccgc cggaccgaac cagaccgcga
     2761 tcttgatgag attggcctgg tcggcggcgt cgacgtctcg gagcggagcc atcagcgaga
     2821 ttgcgcccga aaccgccccg atgcaagttg cgtaggggcc acatgatcat cggactactg
     2881 ctctggggcg ccgcgctggg cggcggattc atccccacac gaaatttcgt gcggcggcgg
     2941 ctgcgcttcg tcgacgccgc gcaaaagccc tcgaccccgg tcgtggccgg cgtcgtcgcc
     3001 gccgcagcgg gcatcaccgc cgccgccctc ccgatcatca cggtcccgat gggcgttctc
     3061 ttcgggatcg gcgtcggggc cgggtgggcg agcggccaga aggctccccc cgaatcctga
     3121 ccggctcccc ttcttcatac tccccccggg ctgtcgctta ggttggtggc gcccggaatc
     3181 ccgatccgtt cgtgccgaaa cgcgtaagct cagggagccc gcgcgtttcc gtccaccggc
     3241 cgggtaccag ccgctttccc caggaggagt cgtcatgcag cgagtcgccg tcgccatcgc
     3301 ggtcggggtc gccgtcctgg cacccagcgc ccgcgcccag gacaaccagt ggcgcacgca
     3361 ggtcgag
//
LOCUS       DBNGZW010000576         5859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_870300, whole genome shotgun sequence.
ACCESSION   DBNGZW010000576 DBNGZW010000000
VERSION     DBNGZW010000576.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5859
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_870300"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="AC16JC_13615"
     CDS             <1..573
                     /locus_tag="AC16JC_13615"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR5165781.1"
                     /translation="NLVSPARRPLAGRIVVDMAATLRAWVTGQILAMLVFAVLTAVGL
                     WALGVPYWLAFGLFTGVAALVPFFGTIVSTVLPVLFVVGPGSSALHAIAVLALGVVVH
                     ILGSNLIVPWIMARSVAVPPAITIASVLAMGTLLGPVGLVVAVPVVALALVLVRHVLH
                     GELYGEGTTFRSAGLRASRGRLPPTLTSTP"
     gene            570..1652
                     /locus_tag="AC16JC_13620"
     CDS             570..1652
                     /locus_tag="AC16JC_13620"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020382775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase DgcN family protein"
                     /protein_id="HIR5165782.1"
                     /translation="MTAPDDHRFLILADGQFGPMSSKTANSCIRYFPERIVGVFDRAQ
                     AGKTVQDVLGFGGAIPVVDDFAQGLSLGGGATAVLVGIAPAGGQLPDEWRAWLKLAIE
                     RKREVWSGLHTFIADDPDLGGLARRLGVRILDARRPPAHLPIADGRAAEVDAYVVQAV
                     GSDCNVGKMTAMLEVRNALVRQGRKARFAATGQTGIFIEGWGTAVDAVVADFIAGAAE
                     DLVLTAARDADIVLVEGQGSLIHPGYSGVTLGLLHGAMPRGLILCHQSSREFVGDYHG
                     REPWVKIPPLRDLIRIYEDAARPVFPTRVLGICLNTFDLDEVAARRAVAAATAETGLP
                     AADPVRFDPAPLAEAIAQAADRQRAR"
     gene            1725..2423
                     /locus_tag="AC16JC_13625"
     CDS             1725..2423
                     /locus_tag="AC16JC_13625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165783.1"
                     /translation="MDFLRSVRAEWPVLRIRLLRTRLGLWLMLVVAALLWIERAAPAS
                     DPLTSILQAGTLGAVLCVGYLAGSGADRAALLLPLLHPTTPGALVLGRWAAATGGAAL
                     AVLAVAAHAAWTTGAVAASLSAALAGLATAAAIASCSLVLVLTGGNVLAALFFVWLAL
                     MGSAPPETLIRVSHPGSIRVALAAMLEVAPAPWRYRAIATGDPGALLHAVACVALGLA
                     TARTLVGRRPVSTG"
     gene            2420..3328
                     /locus_tag="AC16JC_13630"
     CDS             2420..3328
                     /locus_tag="AC16JC_13630"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5165784.1"
                     /translation="MTIPLQFRSVRRVFRRAFGRPPIVAIADATFELAAGEITCLVGA
                     AGAGKTTLLRLGAGLLRPDTGVVLVAGTPPHGPQGPVVRRLVGFAPRDAVFPPAFTVR
                     EVLEYCACRHAAGRDSRALVRDATELAGLEPFLSCRAATLALAAARRLLLAQCALGGR
                     RLLLLDEPFAGLDAISRRDLSDRLQRCGAAGATVLLSSSDPVGLERLADRVLVLRAGR
                     VVRDAPATVLLGGRVLEVIMDAPPHDPPPGFRVTATGLETDLGHRSAEAALAVCRAHR
                     LGIRASRVRLKGLDDALFDPLEAVPR"
     gene            3362..4270
                     /locus_tag="AC16JC_13635"
     CDS             3362..4270
                     /locus_tag="AC16JC_13635"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR5165785.1"
                     /translation="MPLLLSVLFPDVFHRVLAFLRLDDAELVRKLLQAGVIIALAWAA
                     RWLVRGLARRIAHAAHAGAQGRVTEVERRGHTLADLVQSVGGTVVVLAAVLMVLDLFI
                     NIGPLVAGLGVVGLAVSFGAQTLVKDVIAGFFLLLENHYGVGDVVRIANVEGTVERVT
                     LRSTVLRDGHGVVHFVPNGQVDVVSNLTRQWSRAVVDVGVPYSEDVDRAMQVLREVLR
                     EFGNDASWRDRVVGEPTVAGVEALGESRVELRAWVNVVPGAHGDVERELRRRIKNRFD
                     KEGIEIPVPPRVVRMVAGEEPKAATP"
     gene            4267..5685
                     /gene="cysS"
                     /locus_tag="AC16JC_13640"
     CDS             4267..5685
                     /gene="cysS"
                     /locus_tag="AC16JC_13640"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HIR5165786.1"
                     /translation="MTLVLHNTMTRAVEPFTPLHAPRVTFYTCGPTVWNYAHIGNFRT
                     FLFQDLLRRWLEQSGYDVFHIMNLTDVDDRTIKAAAAAQASLVAHTEPFVRAFFEDRD
                     FLRIKPAHVYPRATTSIPAMVRLVEQLLAKGVAYQGDDGSVYFAIGRFPAYGRLSRLD
                     TREIKVGARVASDEYAKENPSDFALWKKADGLDEQVGAAWDAPFGRGRPGWHLECSAM
                     SLDEIGRRFGVRTLDIHAGGVDLIFPHHEDEIAQSEAVTGEPFARYWLHGAFMQVGGT
                     KMSKRFGNFLTVRDLQEEGVDAGTFRLLVFQTHYRKELDYNDAALAAARDGSRRLDIF
                     RERLRTAGGSPEDERFRALAGTLKTRVTEALDDDLNAPRAVAALFDFVTEGNRLLDAG
                     VRPGESVLAAWRWVDEVLDLCPSRPPADDELVRWAEERIAARTRARAARDFKEADRIR
                     EELRARGVEIEDTPGGTKWRRA"
BASE COUNT          729 a         2037 c         2152 g          941 t
ORIGIN      
        1 aacctcgtga gtccggcccg ccgcccgctg gccggccgca tcgtcgtcga catggccgcg
       61 acgctccggg cctgggtgac ggggcagatc ctcgcgatgc tggtcttcgc cgtcctgacc
      121 gccgtcggcc tatgggcgct gggcgtcccc tattggctcg ccttcgggct gttcaccggc
      181 gtcgcggcgc tggtcccgtt cttcggaacc atcgtgtcga cggtcctgcc ggtgctgttc
      241 gtcgtgggcc ccggcagcag cgcgctgcat gccatcgcgg tcctcgcact cggcgtggtg
      301 gtgcacatcc tgggctcgaa cctgatcgtc ccctggatca tggcccggtc ggtcgccgtg
      361 ccgcccgcca tcaccatcgc cagtgtgctc gccatgggca cgctgttggg ccccgtcgga
      421 ctggtcgtcg ccgtgccggt cgtggcgctg gccctggtcc tcgtccgcca cgtgctgcac
      481 ggcgagctgt acggtgaagg cacgaccttc cggtcggccg ggctgcgcgc cagtcgcggc
      541 cgcctgccgc ccaccctcac ctccactcca tgacagcgcc ggacgaccat cgatttctga
      601 tcctggcgga cgggcagttc ggtcccatgt cgtcgaagac cgccaattcc tgcatccgct
      661 acttccccga gcgcatcgtc ggcgtgttcg accgggcgca ggccgggaag accgttcagg
      721 acgtgctcgg attcggcggc gcgatcccgg tggtggacga cttcgcgcag ggactgtcgc
      781 tcgggggcgg ggcgaccgcg gtgctcgtgg gcatcgcccc ggccggcggc cagctgcccg
      841 acgaatggcg cgcctggctc aagctcgcca tcgagcggaa gcgtgaggtc tggagcgggc
      901 tgcacacctt catcgccgac gatccggatc tgggggggct ggcgcgccgg ctcggcgtcc
      961 ggatcctcga cgcccgcagg ccgcccgcgc acctgccgat cgccgacgga cgcgccgccg
     1021 aggtggacgc ctacgtggta caggccgtcg gctccgactg caacgtcggc aagatgaccg
     1081 ccatgctcga ggtgcggaac gccctggtgc gtcaggggcg caaggcgcgc ttcgccgcga
     1141 ccgggcagac cggaatcttc atcgaaggct gggggacggc ggtagacgcg gtcgtggccg
     1201 acttcatcgc cggcgccgcg gaggacctcg tgctcaccgc cgcacgggac gccgacatcg
     1261 tgctcgtgga ggggcagggg agtctgatcc acccaggcta ctcgggcgtc accctgggcc
     1321 tgctgcacgg cgcgatgccg cgcgggctga tcctctgcca ccagtcttcc cgcgagttcg
     1381 tcggcgatta ccacggccgc gagccgtggg tgaagatccc gccgctccgc gacctgatcc
     1441 ggatctacga ggacgccgcg cgaccggtct ttcccacccg ggtcctgggc atctgcctca
     1501 ataccttcga tctcgacgag gtcgcggcac gccgtgccgt cgcagccgcc acggccgaaa
     1561 ccgggctgcc ggcagcggac ccggtgcgct tcgatccggc tcccctggcc gaggcgatcg
     1621 cgcaggccgc ggaccgacag cgggcacgct gagtcgcgtt cgttcgatcc cacccgcaat
     1681 cattcggtag cactccgccg cggccggcgt gacatcgtgc ggtcgtggat ttccttcgca
     1741 gcgtccgggc cgaatggccg gtgctccgca tccggctgct gcgcacccgc ctcggcctgt
     1801 ggttgatgct ggtcgtcgcg gcgttgctgt ggatcgagcg agccgcaccg gcgtccgatc
     1861 cgctcacctc gatcctgcag gcgggcacac tcggtgccgt gttgtgcgtg ggctatctcg
     1921 cgggctcggg agccgaccgg gcggcgctcc tcctgccgct gttgcacccc acgactccgg
     1981 gggcgctcgt gctgggccgc tgggcggccg ccacgggtgg agcggcactc gccgtcctcg
     2041 ccgtcgcggc gcacgccgcc tggacgaccg gcgccgtcgc ggccagtctc agtgccgcgc
     2101 tggccggtct cgcgacggct gcggccatcg cgtcctgctc gctcgtcctg gtcctgacgg
     2161 gcgggaacgt cctcgccgcc ctgttcttcg tctggctggc gctcatgggg agcgctcctc
     2221 cggagacgct catccgggtc tcgcacccgg gttcgatccg cgtggcactc gcggccatgc
     2281 tggaggtggc cccggccccg tggcgctacc gcgccatcgc caccggcgat ccgggagcgc
     2341 tgctgcatgc cgtcgcgtgc gtggcgctcg gcctggccac cgcccgcact ctggtggggc
     2401 ggcgcccggt gtccaccgga tgacgatccc gctgcagttc cgctccgtac gacgggtctt
     2461 ccggagagcg ttcggccggc cgccgattgt cgcgatcgcc gacgcgacgt tcgaattggc
     2521 ggccggagaa atcacctgcc tggtgggagc ggcgggagcg ggcaagacca cgttgctgcg
     2581 gctcggcgcc ggcctgctgc gcccagatac gggcgtcgtc ctcgtcgccg gcacgccgcc
     2641 gcacggccct cagggcccgg tggtgcggcg tctcgtggga ttcgcgccac gcgatgccgt
     2701 tttccctccg gccttcaccg ttcgcgaggt gctggagtac tgcgcgtgtc gccacgcggc
     2761 gggacgggac tcgcgagcgc tggtgcgtga tgcgacggag ctcgcgggac tggagccgtt
     2821 cctctcgtgc cgggcggcca cgctggcgct cgccgccgcg cggcgtctcc tcctggcgca
     2881 gtgcgcgctg gggggccggc ggctgctgct cctcgacgag ccgttcgccg ggctcgacgc
     2941 gatcagccgg cgcgacctga gcgaccggct gcagcgctgc ggcgccgccg gcgccacggt
     3001 gttgctctcg tcctccgatc ccgtgggcct cgagcggctg gcggaccggg tgttggtgct
     3061 gcgggccggc cgggtggtgc gtgacgcgcc cgcgaccgtc ctgctcgggg gtcgggtgct
     3121 cgaagtgatc atggacgcgc cgccgcacga cccacccccc ggattccgcg tcacggcgac
     3181 ggggctggag accgatctcg ggcaccgcag cgcggaggcc gccctggccg tgtgtcgggc
     3241 ccatcggctg gggattcggg cttcccgcgt gcgcctgaaa ggactggacg acgccctgtt
     3301 cgacccgctg gaggccgtgc cgcgttgacg ccccgatgag gggtttctac gttacgcggg
     3361 catgcccctc ctcctcagcg tcctcttccc cgacgtcttc catcgcgtcc tggcgttcct
     3421 ccggctcgac gacgcggagc tggttcgcaa gctgctccag gcgggggtca tcatcgcgct
     3481 cgcctgggcg gcgcgctggc tggtccgcgg actggcccgg cgtatcgcgc acgccgccca
     3541 cgccggcgcc cagggccggg tcaccgaggt ggagcggcgc ggtcacacgc tggcggatct
     3601 ggtccagagc gtgggcggca cggtggtggt gctggcggcc gtgctcatgg tgctcgacct
     3661 gttcatcaac atcggcccgc tggtggccgg attgggcgtc gtggggctcg ccgtgtcctt
     3721 cggagcgcag acgctcgtga aggacgtgat cgcggggttc ttcctgctgc tggaaaatca
     3781 ttacggcgtg ggcgacgtgg tccgcatcgc caacgtcgag ggcaccgtgg agcgggtcac
     3841 gctgcgctcg acggtgctgc gcgacgggca cggcgtggtc catttcgttc ccaacggaca
     3901 ggtcgatgtg gtttccaatc tgacgcgaca gtggtcgcgc gccgtcgtgg acgtgggtgt
     3961 tccctacagt gaggatgtgg accgggcgat gcaggtgctg cgcgaggtac tgcgggagtt
     4021 cgggaacgat gcgtcgtggc gcgaccgcgt ggtgggcgag cccacggtgg ccggggtgga
     4081 ggccctgggc gagtcccggg tggagctgcg ggcgtgggtg aacgtggttc cgggcgcgca
     4141 cggcgatgtc gagcgggagc tgcgccggcg catcaagaac cgcttcgaca aggaagggat
     4201 cgagattccg gtgccgccac gggtggtacg gatggtcgcg ggcgaggagc cgaaggcggc
     4261 cacgccatga cgctggtcct ccacaacacg atgacccggg cggtggagcc cttcacgccg
     4321 ctgcacgcgc cgcgcgtgac gttctacacc tgcggtccca ccgtgtggaa ctacgcccac
     4381 atcggcaatt tccgcacgtt cctgttccag gacctgctgc gccgctggct cgagcagtcg
     4441 ggatacgacg tcttccacat catgaacctc accgacgtgg acgaccggac gatcaaggcg
     4501 gccgcggcgg cgcaggcttc gctcgtggca cacaccgagc cgttcgtccg cgccttcttc
     4561 gaagaccgcg acttcctgcg catcaagccg gcgcacgtct acccccgcgc cacgacctcg
     4621 atcccggcaa tggtgcggtt ggtggagcag ctgctcgcca agggggtcgc ctaccagggg
     4681 gacgacggct cggtgtactt cgcgatcggc cggtttccgg cgtacgggcg tctgtcccgg
     4741 ctcgacacgc gggagatcaa ggtcggcgca cgggtcgcga gcgacgaata cgccaaggag
     4801 aatccgagcg atttcgccct ctggaagaag gcggacgggc tggatgagca ggtgggcgcc
     4861 gcgtgggatg cgcccttcgg gcgcggtcgt ccggggtggc accttgagtg ctcggccatg
     4921 tcgctcgacg agatcgggcg ccgcttcggc gtgcgcaccc tcgacatcca cgccggcggc
     4981 gtggacctga tcttccctca tcacgaggac gagatcgccc agtcggaggc cgtgacgggg
     5041 gagccgttcg cgcgctactg gctgcacggg gcgttcatgc aggtgggtgg gaccaagatg
     5101 tccaagcgct tcggcaactt cctcacggtg cgggacctgc aggaggaggg cgtggacgcc
     5161 ggcacgttcc gcctgctcgt gttccagacg cactatcgca aggagctgga ctacaacgac
     5221 gcggcgctgg cggcggcccg ggacggctcg cgacggctcg acattttccg ggagcggctg
     5281 cggacggccg gcgggtcgcc ggaggacgag cggtttcgcg cgctcgccgg gaccctgaag
     5341 acgcgtgtga ccgaggcgct ggacgacgac ctcaacgccc cgcgggccgt ggcggccctg
     5401 ttcgatttcg tgacggaagg caaccggctg ctcgatgccg gcgtgcggcc gggggagagc
     5461 gtgctggccg cctggcgatg ggtcgacgaa gtgctggatc tgtgcccgtc gcggccgccg
     5521 gcggacgacg agctggtgcg gtgggccgag gagcgcatcg ccgcccgcac gcgggcgcgc
     5581 gcggcacggg atttcaagga agccgaccgg atccgggagg agctgcgcgc ccggggcgtc
     5641 gagatcgagg acaccccggg cgggacgaag tggcggcggg cctaggtcac aggccgggca
     5701 ccaccatgtc cggacgccac gcgaatcggg ccccgaccca cccgatgacg aggtacagca
     5761 cgatcgcgcc gatgatcacc acgacggtat accccaccgc cttgtcctgc ggggccttca
     5821 tgacgaccgg caggccgaga tacagcagga agaggctgt
//
LOCUS       DBNGZW010000577         1522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_53085, whole genome shotgun sequence.
ACCESSION   DBNGZW010000577 DBNGZW010000000
VERSION     DBNGZW010000577.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1522
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_53085"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="AC16JC_13645"
     CDS             complement(<1..405)
                     /locus_tag="AC16JC_13645"
                     /inference="COORDINATES: protein motif:HMM:NF014586.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine nucleotide alpha-hydrolase family
                     protein"
                     /protein_id="HIR5165787.1"
                     /translation="MSERLEILRAAVRRFESALVGFSGGVDSALLAVVLRQELGPVRM
                     LAAVGRSPAVAADQWEAALTLAARFDVPLVEVSTSELDDPRYAANPTNRCFFCKTELW
                     ARLGELARQRGLAVVCDGTNADDLGEHRPGAAA"
     gene            571..1050
                     /locus_tag="AC16JC_13650"
     CDS             571..1050
                     /locus_tag="AC16JC_13650"
                     /inference="COORDINATES: protein motif:HMM:NF013224.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HIR5165788.1"
                     /translation="MSVKPGLDATDRRILRLLAADARSSYQGIADEVGLSRPAVMERV
                     KRLEEAGYITGYGAHLDRGKLGFPVTAFVAVRYGNTSYVGDEPRMREMIKHPGILECH
                     HVAGEDCYILKVVAPDLEGVQQVLRTLSTGQRSFNTRTTIVLSTVFEKPGLMVEDLD"
     gene            1050..>1522
                     /locus_tag="AC16JC_13655"
     CDS             1050..>1522
                     /locus_tag="AC16JC_13655"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR5165789.1"
                     /translation="MAGLRGKALVAWLLVCVFWGSTYLAIRVGVGVLPPFLFAGLRFV
                     AAGALLGAGALAFGDHLPRRAVDWQRAAIAGVLLLAGGNAFVVWAEQYTPSGVTSVFV
                     VTVALWMAFFDSVIPGGSGSLGWRVVLGLFTGFLGTALLVGANPREILAADLRGP"
BASE COUNT          226 a          521 c          507 g          268 t
ORIGIN      
        1 ggccgccgcg cccggccgat gctcccccag gtcatccgcg ttggtgccgt cgcacacgac
       61 tgcgagtccg cgctgccggg ccaattcgcc cagccgcgcc cacagctcgg tcttgcagaa
      121 gaaacaacgg ttggtcgggt tggcggcgta gcgcggatcg tcgagctcgc tggtcgatac
      181 ttccaccagc ggaacgtcga agcgcgcagc cagggtcagg gccgcctccc actggtccgc
      241 ggccaccgcc gggctccgcc cgaccgcggc gagcatccgc accggcccca gctcctggcg
      301 cagcaccacc gcgagcaacg ccgaatccac cccgccggag aaacccacca gggcggactc
      361 gaaccgtcgc accgccgccc gcaggatctc cagccgctcg ctcatgggcc aaacgtagcg
      421 aggggcagtg tcctatctca aggacaggtc gaaatttgat gacaagaacc ggatggttcg
      481 tctgccggaa ccgggtatta tggtcttgcg ttagtataga tgccgatttt atctacgaat
      541 cgtccgacat tttcgattcg aggctacgga atgtccgtca aacccggcct ggacgcgact
      601 gatcgccgca tcctccgcct gctggcggcc gacgcccgct cgagctacca gggcatcgcc
      661 gacgaagtcg gactctcccg tccggccgtg atggagcggg tcaagcgcct cgaagaggcg
      721 ggctacatca cggggtacgg cgcccacctg gatcgcggca aactcggctt ccccgtcacc
      781 gcgttcgtcg cggtccggta cggcaacacg agctacgtcg gggacgagcc gcggatgcgg
      841 gagatgatca agcatcccgg catcctggag tgccatcacg tcgccggcga agactgttac
      901 atcctgaagg tcgtggcgcc cgatctcgaa ggcgtccagc aggtcctgcg gacgctgtcc
      961 acgggtcagc ggtcgttcaa cacgcgcacg accatcgtat tgtccacggt gttcgagaag
     1021 cccgggctca tggtggagga cctcgactga tggcgggttt gcgcggcaag gccctggtcg
     1081 cgtggctgct ggtctgcgtc ttctggggat cgacgtacct ggcgatcagg gtcggcgtgg
     1141 gcgtgttgcc cccgttcctg ttcgcgggac tgcggttcgt cgccgccggt gcactgctcg
     1201 gcgcaggcgc gctggcgttc ggcgatcacc tcccgcggcg cgccgtggat tggcagcggg
     1261 cggccatcgc gggcgtccta ctgctcgcgg gcggcaacgc cttcgtggtc tgggccgagc
     1321 agtatacgcc ctcgggcgtg accagcgtct tcgtggtaac cgtcgcgctc tggatggcgt
     1381 tcttcgactc ggtgatcccc ggcggctccg ggtccctggg ctggcgcgtc gtgctcggct
     1441 tgttcaccgg cttcctgggt accgccctgc tcgtcggcgc taatccgcgc gaaatcctcg
     1501 cggcggattt gcgcggcccg at
//
LOCUS       DBNGZW010000578         4526 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_449185, whole genome shotgun sequence.
ACCESSION   DBNGZW010000578 DBNGZW010000000
VERSION     DBNGZW010000578.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4526
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_449185"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="AC16JC_13660"
     CDS             <1..701
                     /locus_tag="AC16JC_13660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165790.1"
                     /translation="AVSESPNHEMSAPAASIQAIRVTGGGPVIQILPGPFPFPDSAAP
                     ILQQFGAEREGLESQVKDGPYSNNVTPIAGSFTFTALDEGAVRDSVDGLATFTTQDGA
                     RWKLVLDRVQTKDVVHHPRFGGVIMGLYYHGNTAVHTPLVPTINSAVALWAVGHLYKN
                     DALLTDNAMVHVMLLSRTRRPGDFALACWDCSKNKIEELQLQILPGPGEPKFDAPGGF
                     LFVNWEKSSSRKQS"
     gene            715..1152
                     /locus_tag="AC16JC_13665"
     CDS             715..1152
                     /locus_tag="AC16JC_13665"
                     /inference="COORDINATES: protein motif:HMM:NF012355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5165791.1"
                     /translation="MRFRTAIVGFIGLASLAGCSGYNGSGPYGGGTGGGQGGGGYGGG
                     GGGGGGGPVGAVTLGPNIQFVSSHNGSQNPAVDTIPVGSSMTWTWTGSASHSVESEGS
                     TSFASSGIKTGSGTYAVQFTAPGTYQYDCAVHGTAMSGTIVVQ"
     gene            1296..1658
                     /locus_tag="AC16JC_13670"
     CDS             1296..1658
                     /locus_tag="AC16JC_13670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165792.1"
                     /translation="MSTVLTRRGRFPLRLWVSGMAVLFFLACRGTTPIKTLLDDPQQF
                     DGKTVRIAGQVEGSVGALGYGAYQVNDGTGTLSIVSEGGGVPRQGAKVGVEGTFRSAY
                     TLGTKTLAVLVEQHRYTP"
     gene            1709..2404
                     /locus_tag="AC16JC_13675"
     CDS             1709..2404
                     /locus_tag="AC16JC_13675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR5165793.1"
                     /translation="MLLRGPVPRLLRAALYAAAALVVFLALYVAFVRPWHMHWGATAA
                     EISRAMPGDTLVIDPIEVTTRAITINAWPSHVWPWLAQMGKGRGGLYSYDWLDRVFGV
                     LDAPSSDTLIPRFQSIKPGDTIPVGGSSGWPVAVARPNHLLLLDVHQAGAHVTWAFAL
                     EPSSPSQTRLIMRVRSRLPHSWRLPFLVAVLDPAEFLMVRRQLIGLRERAEQLARTDT
                     AYARAGVRPGPPK"
     gene            2500..3723
                     /locus_tag="AC16JC_13680"
     CDS             2500..3723
                     /locus_tag="AC16JC_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005031603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5165794.1"
                     /translation="MTDPYAALRVPDFRRFIGFLAAQTLATMMQGVAVGWQLYTVTHD
                     PLSLGLVGLAEALPFVGFALPAGHLADRLDRRRLSLAALFVLALCSATLLAFTGLGLV
                     RADTAWLVYAAIFVSGIARSYLQPTRTALAADLVPRELYLNSITWRSVTWQSAAVAGP
                     AAGGLLVALGGPELTYGVAAVLLWAAWASLLRVAPHPHPAADPGGSGMGGVLQGVRFV
                     LTQPVLLGAQTLDLFSVLFAGAEALLPIFATDILHVGAAGLGVLRAAPAAGSVLMSLY
                     QSYRPLHGRMGRVLLTSVALYGLCISGFGLSRSFPLSVALLLVSGIFDNISVVLRSTL
                     LQTLTPRHLLGRVAGVNAIFIGSSNEIGAFESGVAARALGTVPAVLLGGFVPIVVVAL
                     IAWRVPELRRLERVA"
     gene            3720..>4526
                     /locus_tag="AC16JC_13685"
     CDS             3720..>4526
                     /locus_tag="AC16JC_13685"
                     /inference="COORDINATES: protein motif:HMM:NF012705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HIR5165795.1"
                     /translation="MKPVERLPFALRRPWILARDLLQSRSRPDLERLQQVGNPESFVW
                     RILPHAARTFTACIAVLPAEPARAMAVAYLYCRMLDTYEDLMPEPAEREAALAAFSTR
                     FRVGDGAPPQPAPPIATTHARDVRDQTHVVLVNRCHLVDGVFAKLHPLVRAHIADLVA
                     SMGAGMLWSSRTFREQGGVLQTGDQLARYCRTVIGLPFLFAVRLLQLRRTGNAAVSDE
                     LREDCLHAGEMVQLANVTRDIEKDLRRGVAYHPALRADLGRADFENAGLRA"
BASE COUNT          636 a         1569 c         1550 g          771 t
ORIGIN      
        1 gggccgtgag tgaatcgcct aatcatgaga tgagcgcgcc ggcggcgagc atccaggcga
       61 tccgggtcac gggcgggggg cccgtcatcc agatcctgcc cggtcccttc ccctttcccg
      121 acagtgccgc cccgattctc cagcagttcg gcgccgagcg ggaagggctc gagtcgcagg
      181 tgaaggacgg tccctattcg aacaatgtga ccccgattgc gggcagcttc acgttcacgg
      241 cgttggacga gggcgcagtg cgtgactcgg tcgatggcct cgccacattc accacccagg
      301 acggggcgcg ctggaaactc gtgctcgacc gcgtgcagac gaaagacgtg gtgcatcatc
      361 cgcgcttcgg cggcgtgatc atgggcctct actaccacgg gaacaccgcc gtacacacgc
      421 cgctcgtccc gacgatcaac agcgccgtgg cgctgtgggc ggtcgggcac ctctacaaga
      481 acgacgccct cttgaccgac aatgcgatgg tgcatgtgat gctgctgtcg cgcacccgtc
      541 ggccgggcga tttcgcgctc gcgtgttggg attgctcgaa gaacaagatc gaagagctgc
      601 agctccaaat cctacccgga ccaggcgagc cgaagttcga cgcgcccggc gggttcctgt
      661 tcgtcaactg ggaaaagtcc agctcgcgca agcagagctg aggaggatca accaatgcgt
      721 ttccgcacag caatcgtcgg cttcatcggg ctcgcgtcgc tcgcgggctg cagcggctac
      781 aacggcagcg gcccctatgg gggcggtacc ggcggaggac agggtggcgg tggttacggc
      841 ggtggcgggg gtggcggggg tggcggcccc gtgggggcgg tcacgctcgg ccccaacatc
      901 caattcgtga gtagccacaa cgggagccag aatccggccg tggacacgat cccggtcggg
      961 agctccatga cctggacctg gaccggatcc gcgtcccaca gcgtcgagtc cgaaggatcg
     1021 acgagcttcg ccagcagcgg catcaagacc ggcagcggca cgtatgcggt ccagttcacc
     1081 gcgccgggca cataccagta cgactgcgcc gtgcacggca cggcgatgtc gggcaccatc
     1141 gtcgtccagt agtgcgggtt gatgggtctc cgcatccgag tcggatgcgg gggcccaggc
     1201 tggccgagga acctggcccg tcctgtggtg tcctgggagc gtggggcagc aagcctgttt
     1261 gcgctgcagc gtttcacttc caaggagctt cgatcatgtc gaccgtactg acacgccgtg
     1321 gcaggtttcc gctgcgcctg tgggtctccg ggatggccgt gctgttcttt ctggcctgca
     1381 gaggaaccac gcctatcaag acgctgctcg atgatccgca acagttcgac ggcaagaccg
     1441 tccgcattgc ggggcaggtc gaaggttccg taggcgccct gggctacggg gcctaccagg
     1501 tgaacgacgg aaccgggacg ctgtccatcg tgagcgaggg cggtggggta ccgcggcagg
     1561 gagccaaagt cggggtagaa ggaacgttcc gatccgcata cacgctcggc accaagactc
     1621 tggccgtgct cgtagaacag catcgctaca ccccctgaga acggaacctg atgccccccg
     1681 cggcgagcgg atacatggtc ccgggcacat gctcctgagg ggcccggtac cccgcctgct
     1741 tcgcgcggcg ctctacgccg cggccgccct cgtcgtcttc ctggcgctct atgtcgcgtt
     1801 cgtccgaccc tggcacatgc attggggcgc gaccgccgcc gaaatcagtc gagccatgcc
     1861 cggcgatacg ctcgtgatcg atccgatcga agtcaccaca cgcgccatca cgatcaacgc
     1921 gtggccgtcc cacgtctggc cctggctcgc ccagatgggc aaggggcgcg gcgggttgta
     1981 cagctacgat tggctcgatc gcgtcttcgg ggtgctcgat gctcccagct ccgacacgct
     2041 gatccctcgc ttccagtcga tcaagcccgg cgacacgata ccggttggcg gcagctctgg
     2101 atggcccgtg gccgtggcca ggccgaacca cctgctcctg ctcgacgtcc atcaggccgg
     2161 ggcgcacgtc acgtgggcct ttgcgctgga gccgagttcc ccgagccaga cacggttgat
     2221 catgcgcgtg cggagccggc tgccgcactc ctggcggtta ccgttcctgg tcgccgtgct
     2281 cgacccggcc gaattcctga tggtccgacg gcagttgatt ggactccggg aacgggcgga
     2341 gcagctggcg cgcacggaca ccgcgtatgc tcgggcagga gtccgacccg gaccgccgaa
     2401 gtaggctgct ccttcaaggt tgggccgagc tgtagggtgg cggtcgagga acggtcaggc
     2461 gtgtgacgcg ccacgactcg cctgttatcg ttcggcgcca tgaccgaccc ctacgccgcg
     2521 cttcgggtcc ccgattttcg ccgcttcatc gggttcctgg cggcccagac gctcgccacc
     2581 atgatgcagg gcgtggccgt cggctggcag ctctacacgg tcacgcacga cccgctctcg
     2641 ctcggcctgg tgggactggc cgaggcgctg cccttcgtcg gcttcgcgct gccggcggga
     2701 cacctcgccg accgcctgga ccggcgacgc ctttcgctcg ccgccctgtt cgtgctcgca
     2761 ctctgcagcg ccaccctgct cgccttcacg gggctcggcc tggtacgcgc cgacacggcg
     2821 tggctggtct acgctgcgat cttcgtcagc ggcatcgcgc gcagctacct gcagccgacc
     2881 cgtaccgccc tcgccgccga tctcgtgccg cgcgagctgt acctcaattc catcacgtgg
     2941 cgcagcgtca cctggcagtc ggccgccgtc gccgggccgg ccgctggcgg cctgctggtc
     3001 gcgctgggcg ggccggagct gacctacggc gtggccgccg tcctcctctg ggccgcctgg
     3061 gcctcgctac tccgggtggc gccccatccc catcccgcgg ccgatccggg cgggagcggg
     3121 atgggcggcg tgctgcaggg cgtgcgcttc gtgctcaccc aaccggtgct gctcggcgcc
     3181 cagacgctgg atctcttctc ggtgctgttc gccggagcgg aggcgctgct gcccatcttc
     3241 gccacggaca tcctgcacgt gggcgcggcg gggctcgggg tgctgcgggc ggcaccggct
     3301 gcgggctcgg tattgatgtc gttgtatcaa tcgtaccgcc cgctgcacgg ccgcatgggg
     3361 cgcgtcctgt tgacgtcggt cgccctgtac ggactgtgca tcagtggatt cgggctgtcg
     3421 cggagcttcc cgttgtcggt cgccctgctg ctcgtgtccg gcatcttcga caacatcagc
     3481 gtggtgctgc gctcgacgct gctccagacg ctcacgccgc ggcacctgct gggccgcgtg
     3541 gccggcgtga acgcgatctt catcgggtcg tccaacgaga tcggcgcgtt cgagtcgggc
     3601 gtcgccgccc gcgcgctcgg caccgtgccg gcggtgctgc tcggcggatt cgtaccgatc
     3661 gtcgtggtcg ccctgatcgc ctggcgcgtc cccgagctac gccggctgga gcgcgtcgcg
     3721 tgaagcccgt cgagcgtctc cccttcgcgc tgcgccgccc ctggatcctc gcccgggatc
     3781 tgctgcagag ccgctcccgc ccggatctcg agcggctgca gcaggtcggc aatcccgagt
     3841 cgttcgtctg gcgcattctc ccccatgcgg cgcgcacgtt cacggcgtgc atcgcggtgc
     3901 tcccggcgga gccagcgcgg gccatggccg tcgcctacct ctattgccgc atgctcgaca
     3961 cctacgagga tctgatgccg gaaccggccg agcgggaggc cgcgctggcg gccttcagca
     4021 cccggtttcg ggtcggggac ggcgccccac cgcagcccgc gccgcccatc gccacgaccc
     4081 acgcgcgcga cgtgcgggat cagacgcacg tggtgctggt gaaccgttgc cacctcgtcg
     4141 acggcgtatt cgcgaagctc cacccgctgg tgcgcgcgca catcgcggac ctggtcgcgt
     4201 cgatgggcgc ggggatgctc tggtcgtcgc gcaccttccg cgagcagggc ggcgtgctgc
     4261 aaacggggga ccagctcgcc cgctactgcc gcacggtcat cggactgccg ttcctgttcg
     4321 cggtccgcct gctccagctg cgcaggaccg ggaatgccgc cgtgtccgac gagctgcgcg
     4381 aggattgcct gcacgcgggc gagatggtgc agctcgccaa cgtcacgcgc gacatcgaga
     4441 aggacctgcg ccggggggtg gcctatcacc ctgccctgcg ggcggatctc ggccgggcgg
     4501 atttcgagaa cgccgggctg cgcgcg
//
LOCUS       DBNGZW010000579         2453 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_325287, whole genome shotgun sequence.
ACCESSION   DBNGZW010000579 DBNGZW010000000
VERSION     DBNGZW010000579.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2453)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2453)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2453
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_325287"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..793)
                     /locus_tag="AC16JC_13690"
     CDS             complement(137..793)
                     /locus_tag="AC16JC_13690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165796.1"
                     /translation="MNRRLIWVALYAIAMAFVEAAVVVYLRALLDATRAGQVPGVEIG
                     REAATIVMLLAVGVLAGGELRERFLVFCLAFGIWDLGYYAWLRVLLGWPPSLLTSDIL
                     FLIPVPWVAPVLAPAIVSVGLVAGSLWLLALRARGAILHFPVWIWALAAGGGALVLLS
                     FTLDYRVVLQGQEPPAFRWPLFGAGVAVGAAALVAGVRSLPGRPPESRNVATESAPPR
                     "
     gene            complement(790..2316)
                     /locus_tag="AC16JC_13695"
     CDS             complement(790..2316)
                     /locus_tag="AC16JC_13695"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5165797.1"
                     /translation="MVATDPPVIATPTVEAESNAGRRLVARAMWIGLWVWPSFTLLDA
                     YMCFVAYPGAPFRAFLAYRVAVELGFISVYRASRRGTTDLKRLYLLQNLCYGSAALVI
                     AIMAVNLGGIRSPYMHGISVVALVRAALVPTHWRRGLKTYARIALAFPLVMGVGAVIS
                     PAARDEWLRAESLIVFASNYVFVLASSCLGLVSGHIVWSTQQQLFRARRIGRYRLEAP
                     IGKGGMGEVWLAWDQSLHRNVALKILRVGTTSSPESVRRFELEAQAAGQLRGPHVVHI
                     FDFGASEDGLYYIAMEYLTGMNLASLVEKFGPLPPARVVHLAIQACLALEEAHAAGII
                     HRDLKPHNLYLSHARDQPDCLKLLDFGIARLRSGDAGSEHLTWTGAMIGTPAYLAPEL
                     WQGEEADERSDIYALGVTLHYLLAGETPHGDWSVVQLQAAHLLSEPPALRLPGNDALS
                     QCLEALLLRCLAPSPAQRVQTARELRAALLALYDPVAWTRADADAFWKRAERERFGEA
                     "
BASE COUNT          460 a          836 c          845 g          312 t
ORIGIN      
        1 attacgccga tgaagctgta ccagacctac cagcggttcg ccgatcaggg cacgacgctc
       61 gagctgcgcg ccctcacagt gggacaggcg tactgggtcg cgatcgagag cttcgacgag
      121 aacggagtgt cgccgctcag cgcggcggtg ccgattcggt agccacgttc cgcgactccg
      181 gcggccgccc cggcaggctc cggactccgg ccacgagagc ggccgcgccg acggccacgc
      241 cggccccgaa caagggccat cgaaacgcgg gcggctcctg accctgaagc acgacccgat
      301 agtccagcgt gaaggacaac agcacgagcg cccctccccc ggccgcgagc gcccatatcc
      361 agaccgggaa atggagtatc gcgccgcgcg cccgcagcgc cagcaaccac aacgaacccg
      421 ccaccagccc cacgctgacg atcgccggtg ccagaaccgg tgcaacccag ggcacgggga
      481 taaggaagag gatatccgag gtgagcagcg acgggggcca tccgagcagc acgcgcagcc
      541 aggcgtagta gcccaggtcc cagatcccga aggccaggca gaagaccaga aagcgctccc
      601 ggagctcgcc gccggccagc acgccgacgg cgaggagcat gacgatcgtg gcggcctccc
      661 ggcctatttc gacgccggga acctgaccgg cccgggtcgc gtccagcagg gcgcgcagat
      721 agaccacgac ggcggcctcg acgaacgcca tggcgatcgc gtagagggcg acccagatga
      781 ggcggcggtt cacgcttccc cgaaccgctc tcgctcggcg cgcttccaga acgcgtcggc
      841 atcagcccgt gtccacgcga ccggatcgta cagcgcgagg agggctgcgc gcagctcgcg
      901 cgctgtctgc acccgctgcg ctggagacgg tgccagacac ctcagcagca gcgcttcgag
      961 acactggctc agtgcatcgt tccccggcag ccgcagggcg ggtggctcag acaggagatg
     1021 tgcagcctgc agctgcacca ccgaccagtc cccgtgcggc gtctcgcccg cgagcagata
     1081 gtggagggtg acgccgagcg cgtagatgtc gctgcgctcg tcggcttcct caccctgcca
     1141 cagctcgggg gcgagataag cgggcgtccc gatcatggcg ccggtccagg tgaggtgctc
     1201 gctgccggca tccccggagc gaaggcgcgc gatgccgaaa tcgaggagct tgaggcagtc
     1261 gggctggtct cgtgcatgcg acaggtagag gttgtgcggc ttgagatccc ggtggatgat
     1321 ccccgcggca tgcgcctcct cgagcgcgag acacgcctga atggccaggt ggacgacgcg
     1381 tgccggcggc agcgggccga acttttcgac gaggctcgcg aggttcatcc cggtgagata
     1441 ttccatggcg atgtagtaca gcccgtcctc gctcgcgccg aagtcgaaga tgtgcacgac
     1501 gtggggaccg cggagctgac ccgcggcctg cgcctcgagc tcgaacctcc gtaccgactc
     1561 ggggctcgac gtggtgccca cgcgcaggat cttgagcgcc acgttccggt gcagagactg
     1621 gtcccacgcc agccagactt cgcccatccc gcccttgccg atgggcgcct cgaggcggta
     1681 gcgcccgatg cggcgcgcac gaaacagctg ctgctgcgtg ctccacacga tgtggccgct
     1741 caccaggccg aggcaggaag aagcgagcac gaacacgtag ttgctcgcga agacgatgag
     1801 cgactccgcg cgcagccact cgtctcgggc ggccggggaa atgacggcac cgacccccat
     1861 caccagcggg aaggccagcg cgatccgggc ataggtcttg agcccgcgcc gccagtgcgt
     1921 cgggacgagc gccgcgcgca cgagcgccac aactgagatg ccgtgcatgt agggactgcg
     1981 gatgccgccg agattgaccg ccatgatggc gatcacgagc gccgcgctgc cgtagcagag
     2041 attctgcagc aggtagaggc gcttgaggtc cgtcgtgccg cgacggctcg cgcgatagac
     2101 ggagatgaac cccagctcaa ccgccacacg atacgcgagg aatgctctga acggggcgcc
     2161 cggatacgcc acgaagcaca tgtacgcgtc gagcagcgta aaggaaggcc agacccagag
     2221 cccgatccac atggcccgcg cgaccaggcg ccggccagcg ttggactcgg cctcgacggt
     2281 cggggtggca ataacgggag ggtcggtggc gaccatgggc gctcgcaacc aaggtacggc
     2341 accgtcaagg gcgccggatt aaggcagctt catggcacgc cgtccagcag cgcgacgagc
     2401 ggctcccttg ccgagcgttc gggcgtatgg tccgcatcga gccgatgaac ggg
//
LOCUS       DBNGZW010000580         2820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1687931, whole genome shotgun sequence.
ACCESSION   DBNGZW010000580 DBNGZW010000000
VERSION     DBNGZW010000580.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1687931"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="AC16JC_13700"
     CDS             <1..425
                     /locus_tag="AC16JC_13700"
                     /inference="COORDINATES: protein motif:HMM:NF013961.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HIR5165798.1"
                     /translation="VAAQARIVAGPDRDPRRVATRLVHWVATAVRRERTDGIGDAAGV
                     LERRRGDANEHTVLFVAMARALGLPARPAAGVVRAAGRTGYYYHSWAEVYLGDWVAVD
                     PTFDQLPADAVHLRLAVGALVRPSDFARLLGRLTLDPS"
     gene            436..1194
                     /locus_tag="AC16JC_13705"
     CDS             436..1194
                     /locus_tag="AC16JC_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014812358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5165799.1"
                     /translation="MIRLEGLTKHYGSFVALDHIALHVPRGVLYGCLGPNGAGKTTML
                     RMIAGILRPTDGTVLIGGDDVQVNPLAAKMRLGFIPDRPFVYDKLTGAEFLRFVAGLY
                     GQDGETVERRVAELLEVFELSSWKDELIEAYSHGMRQKLIISSALIHRPECIVVDEPM
                     VGLDPKGARLLKDIFRQFVERGGTVLMSTHTLEVAEAMCDQIGILQHGRIVAHGTMAE
                     IRRQHAAGDASLEELFLKLTGGHAVRELVEVLGR"
     gene            1194..>2820
                     /locus_tag="AC16JC_13710"
     CDS             1194..>2820
                     /locus_tag="AC16JC_13710"
                     /inference="COORDINATES: protein motif:HMM:NF028259.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC transporter permease subunit"
                     /protein_id="HIR5165800.1"
                     /translation="MQPTLAHVLQPKWLTARQRLRQERAAGGRGKLVVLVLVGGAFWA
                     GVYGVLYFILRYFRGVEELGPLLAGKLLGLVLLSFMTILILSNVITALSSFFLAKDLD
                     LLVSAPIDWLRLYLAKLGETLVHSSWMVALMTVPILAAYGVVYHGGVLFIPYAIAVIL
                     PLLVLPTVLGSALTLVLVNIFPARRTRDLLSIIALGAAGVLILLFRVIRPEQLARPEG
                     FRNLLDFIAVLRTPTSPFLPSEWATRAVMGYLGSKLDPLPLVLLWSTAAAFVTLGAML
                     HRRLFATGFTKAQEGAERFVRGVLWRRTVGQVLFALPVAKREFVLKDVKLFFRDTTQW
                     SQLILLGVLVVVYLFNIKALPLHRGEPVGFFYVTLVSFLNLGLAGFVLAAIAARFIFP
                     AVSLEGRQMWLLRSSPLDLRTLLWSKYWVGTIPLLVLALLLTGLTNLLLDVTPFMMVI
                     SLVVICALTLAIASLALCYGTLYPQFETENAAQIPTSFGGLVFMMTTIALLAAVVFAL
                     WQAVYSYVRAVYERQPVVIDGWMVLWLVVAAIPC"
BASE COUNT          367 a          946 c          983 g          524 t
ORIGIN      
        1 gcgtggcggc gcaggcgcgc atcgtggccg gccccgaccg cgatccccgc cgggttgcga
       61 cccgcctggt gcactgggtg gcgacggcgg tgcgccggga gcggaccgac ggcatcggag
      121 acgcggccgg cgtgctggag cgacgtcgtg gcgacgcgaa cgagcacacc gtactgttcg
      181 tcgccatggc gcgcgccctc gggctcccgg cccgtccggc ggccggagtg gtccgcgcgg
      241 ccggccggac gggttactac tatcactcct gggcagaggt gtacctgggc gactgggtag
      301 cggtcgatcc cacgttcgac cagctcccgg ccgatgcggt ccacctgcga ctcgccgtcg
      361 gggcgctggt ccggcccagc gacttcgccc ggctgctggg acgtctcacg ctcgacccat
      421 catgagccga ctcgcatgat tcgactcgaa ggcctgacca agcactacgg cagcttcgtg
      481 gcgctggatc acatcgcgct gcacgtgccg cgaggggtgc tgtacgggtg cctcggcccc
      541 aacggcgcgg gcaagaccac gatgcttcgg atgatcgccg gcattctccg ccccaccgac
      601 gggaccgtgc tgatcggcgg ggacgacgtc caggtgaacc cgctcgccgc gaaaatgcgg
      661 ctcggcttca ttccagaccg gccgttcgta tacgacaagc tcacgggcgc cgagttcctg
      721 cggttcgtgg cgggattgta cgggcaggac ggcgagacgg tcgaacgccg cgtcgcggag
      781 ctgctggagg tgttcgagct gtccagctgg aaggacgagc tgatcgaggc ctacagccac
      841 ggcatgcgcc agaagctgat catctcgagc gcgctcatcc accggcccga gtgcatcgtg
      901 gtggacgagc cgatggtggg gctcgacccc aagggcgccc gcctgctcaa ggacattttc
      961 cggcagttcg tcgagcgcgg cggcacggtc ctgatgagca cgcacacgct ggaagtggcg
     1021 gaagcgatgt gcgaccagat cggcatcctg cagcacggcc ggatcgtcgc gcacgggacg
     1081 atggccgaga tccggcgcca gcacgcggcc ggggatgcga gcctggagga gctgttcctc
     1141 aagctcacgg gcgggcacgc cgtgcgcgag ctggtggagg tcctggggcg gtgatgcagc
     1201 cgacgctggc gcacgtgctg cagcccaagt ggctgacggc gcggcaacgg ctgcgccagg
     1261 agcgcgctgc gggcgggcgg ggaaagctgg tggtcctcgt gctcgtgggc ggtgccttct
     1321 gggccggcgt gtacggcgtg ctctacttca tcctgcggta cttccggggc gtcgaggagc
     1381 tcggcccgct gctcgccggg aagctgctgg ggctcgtgct gctgtcgttc atgacgatcc
     1441 tgatcctgtc gaacgtcatc acggcgctgt cgagcttctt cctcgccaag gacctcgacc
     1501 tcctcgtctc ggcgccgatc gattggctgc ggctctacct cgccaagctg ggcgagacgc
     1561 tggtgcattc gtcctggatg gtggcgctga tgaccgtccc catcctcgcg gcctacgggg
     1621 tggtgtatca cggcggcgtg ctgttcatcc catacgccat cgccgtgatc ctgccgctgc
     1681 tggtgcttcc cacggtgctg ggcagcgccc tcaccctggt cctggtgaac atcttcccgg
     1741 cccgccgcac gcgcgacctg ctgtcgatca tcgccctggg ggccgcgggg gtgctgatcc
     1801 tcctgtttcg cgtgatccgg ccggagcagc tggcgcgccc cgaggggttc cgcaacctgc
     1861 tcgatttcat cgcggtgctg cgtacgccca cgtccccctt tctgccgagc gagtgggcga
     1921 ccagggccgt catggggtac ctggggagca agctggatcc cctcccgctg gtcctcctgt
     1981 ggagcaccgc cgcggccttc gtcacgctcg gcgcgatgct gcaccggcgg ctgttcgcca
     2041 ccggcttcac caaggcgcag gagggggcgg agcgtttcgt gcgcggcgtc ctgtggcgcc
     2101 ggacggtcgg gcaggtgttg ttcgccctcc ctgtggccaa gcgcgagttc gtcctgaagg
     2161 acgtcaagct gttcttccgc gacactaccc agtggagcca gctcatcctg ctcggcgttc
     2221 tcgtggtggt ctatctcttc aacatcaaag cgctgccgct gcaccggggg gagccggtcg
     2281 gattcttcta tgtgaccctg gtgtcgttcc tcaatctcgg tctggcgggg ttcgtcctgg
     2341 ccgcgatcgc ggcccgcttc attttcccgg cggtgtcgct cgagggacga cagatgtggc
     2401 tgttgcgctc cagtccgctc gatctgcgca cgctgctctg gtccaagtac tgggtgggga
     2461 cgattccgct gctggtgctc gccctcttgc tcaccgggct gaccaacctg ctgctcgacg
     2521 tcaccccgtt catgatggtg atcagcctgg tggtgatctg cgccctgacg ctcgccatcg
     2581 cgtcgctcgc cctgtgctac gggacgctct atccccaatt cgagaccgag aacgccgccc
     2641 agatcccgac ctcgttcggg ggcctcgtct tcatgatgac caccatcgcg ctgctggctg
     2701 ccgtcgtgtt cgccctgtgg caggccgtgt acagctacgt gcgtgcggtg tacgagcgcc
     2761 agccggtggt gatcgacgga tggatggtgt tgtggctcgt cgtcgcggcg atcccctgcg
//
LOCUS       DBNGZW010000581         7795 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1146021, whole genome shotgun sequence.
ACCESSION   DBNGZW010000581 DBNGZW010000000
VERSION     DBNGZW010000581.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1146021"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..829)
                     /locus_tag="AC16JC_13715"
     CDS             complement(<1..829)
                     /locus_tag="AC16JC_13715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5165801.1"
                     /translation="MNISHISVSTLPVLHRFGGAIERRIVEIAHEQARRGHRVSVYSV
                     GDVTETRDLNGVTYHFLKCRTRLPFRHLEFQHHVVRALSRRSDDVLHFHSQPEGAWMS
                     QAVGARKVLSYDFFQFRGGRATPLYHLYKRALRSYDLLLPCSRYCLEESQSFWSFPAS
                     KLRILHNGVNTRQFRPDPEAAARERARLGIDKRVMLYVGRVCEQKGSDVLLRAARALR
                     ERRRDVQLVIAGPIGQFGRREDPDGWGGRIEEVGGLYLGPVEEGRLSAIYNLGDVFVM
                     "
     gene            complement(826..1962)
                     /locus_tag="AC16JC_13720"
     CDS             complement(826..1962)
                     /locus_tag="AC16JC_13720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5165802.1"
                     /translation="MNVCLVGGIFDRPEPIRSRQLVTPETVLLDGFRKAGVTVHAVGH
                     AGFQPSDEYDIIHVHHFGRAALKMAASRGRARVVFTGHNGLIPTGWERSRLRRYAFQY
                     VVDRAEAYIALSRAEARYYARVTSAEKIHTIPNGVPADVFRAAGHRRKRADKARYDVL
                     YVGQLIDWKGVNFLLEAMQQLRKQRDVHLRLVYHNAQLEAELRRQADDLGIAKQVEFV
                     GILDPGALAEEYHQADLLVLPSFADCLPSVITEAFLCGTPVVAGGVCGVPEQIGPYGL
                     AVPPGDSVALADAMERVLAERPRWQDMAAEMRAYAEHTFKPETMVARHLALYRSMIEG
                     RVAPARRRAAWIDPAMRLAVEVYWRRGRAAGRTGRPSLARTIPR"
     gene            complement(1959..3386)
                     /locus_tag="AC16JC_13725"
     CDS             complement(1959..3386)
                     /locus_tag="AC16JC_13725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165803.1"
                     /translation="MRASAVSPMPGATFWVLAINAVALIGFACAPVAEEYPLAVVGIL
                     AFMFVHACLPACRIRMTAPLCPANIAQGYYWIQLVLVTVMVGYYGFTQGALPHLPAKG
                     AINLAIVVHAVSYLSFCVAYQCMSRRTGGPGPGRESTPGTAYLIVPFAVLGVLGFLLA
                     HGGLGGFVDYVSSPIEARLRAEEATTPQGAAGTFLKHFLGFAVVLAWSWWLGKAPRRK
                     PAIVGVTAGVVLGLLVVNFNYNRGTMLGPIVALGAAFSVHAWRIPFKGVVLAGMLALS
                     AAFVFGWYRSTDLDITELSAAAVAATWNAEGVVEFVQIYASGPQMTAYLIESLEDKLY
                     YGKTLLPSLIYPIPVLGKPYRDISGPVIFNHIIYGDTDSIDQIIPLAGELYINFHLAG
                     VVLGGVLLGSLLAWLHGKFVAAPNPVESYAWLMMALWTVFPGSLSVTSQIYVYSFWPI
                     YVYFAARRFWSWNAAMAGHRDRRFA"
     gene            complement(3383..4684)
                     /locus_tag="AC16JC_13730"
     CDS             complement(3383..4684)
                     /locus_tag="AC16JC_13730"
                     /inference="COORDINATES: protein motif:HMM:NF014051.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HIR5165804.1"
                     /translation="MSAAPGDSVARHFAIGAFWALVGAIVSRGLTLASSVVAGRLLGT
                     TGFGEIGMIQSTQGLFGIVAGAGLGLAATKFVAEFRSTDPARTRRCATLATTIALISG
                     AVMALGLLALSGAMASGVLRAPHLTAELQVASGLVLFGTINGVQTGTLVGLGDFRIVA
                     VLTGVRGVCLCGFLLIGIELGGVLGGVVGLVLTEAIAVLANQVALRRLLPESVAGHDR
                     RAAWNELRQMGRFSALSLLGSIATMSAMWFANLVLVAQPDGYASLGVFNAAERWRQLL
                     LFLPASFSPVILTMLSNLHGRSDPDAYRRLFGLNLAVSVAVVVVPSIGVMLCAAPAMG
                     LFGDAYRAGGMTLVILSASSVAVVLNNLLGQVLVSQGAVMGRFVLDVLLAVVLAIASW
                     QLIPIYREQGMALGSLIAFAVTAAALAATTIHFMRARRSVR"
     gene            complement(4780..5448)
                     /locus_tag="AC16JC_13735"
     CDS             complement(4780..5448)
                     /locus_tag="AC16JC_13735"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013174376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR5165805.1"
                     /translation="MTRRVQPGKRLLDVAVSVVVLVLLSPLYALVAALVMAGLGRPVL
                     FRQQRPGLHGRPFELLKFRTMTEEREADGRLKPDPERLSRVGRYLRKTSLDELPQLWN
                     VVRGEMSLVGPRPLLMEYVPNYTPEQQRRHEVLPGITGWAQIHGRNAVVFSDRLKLDV
                     WYVDHWSLALDLKILRRTVLKVVRSSGVRLEQPLEEVDDVGLHPDTRRKAAVASVPSE
                     REAS"
     gene            complement(5445..6701)
                     /locus_tag="AC16JC_13740"
     CDS             complement(5445..6701)
                     /locus_tag="AC16JC_13740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5165806.1"
                     /translation="MKTEPSVNVAIIGAGPYGLAAAAHLRAAGVETCVFGETMEFWET
                     QMPMGMCLRSSWAASHIGHPRRELTLDEFSASERSSISKPIPLDRFVGYGRWFQRHVA
                     PDVDRRRVVRIEREAKGFRLRLDDGDAVLARRVVVAAGISRFGWRPPQFDGLSKELAS
                     HSGEHRDLRHFSGRRVVVVGGGQSALESAALLQEAGAEVEVIARQERIRWLDQRAGWL
                     KSHRNPLRSLLYPPTDVGPPILNRIVAAPDVFRRLPRAMRERIAFRSIRPAGAGWLAG
                     RLHDVPIRTSRVVEAARRAEDGVEVRLDDGSRRNSDHVLICTGYRVDIARYGFLTPDL
                     LSSVRTADGYPLLTDGFESSLAGLHFLGAAAAWSYGPLCRFVSGTAFSGRALARRIAG
                     DRAVMSPAAVPDHERIGAVRAEPFSS"
     gene            complement(6722..>7795)
                     /locus_tag="AC16JC_13745"
     CDS             complement(6722..>7795)
                     /locus_tag="AC16JC_13745"
                     /inference="COORDINATES: protein motif:HMM:NF009404.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR5165807.1"
                     /translation="VTCAGRRSHTIAAFKQAVRGRGLVFACDSSGDAPALQEADDSFL
                     VPLLGAAGYLDALLAICRERRVGLLVPTLEPELPLLAEARSRFREAGTVALVSSSKVV
                     ATCYDKLETARFLAALNLPVPATWLSLDAASAALARGELRLPLVVKPRWGVSSIGLAV
                     AEDDEELGLCFRLAAKHVARSSFAEVSATDPERCVLIQECLDGVEFGLDIVNDLEGRH
                     VCTFIKRKLRMRAGQNDRAVTVQDSRLEELGRTIGESLGHVGVLDCDVLVTAAGCHVI
                     DLNPRFGGGYPFSHVAGANVPAALVAWAAGAEPEASCFDCAPGVTAARSDSFVVTRRV
                     ADSGRAASLASTQNPRRTVQWKS"
BASE COUNT         1400 a         2846 c         2468 g         1081 t
ORIGIN      
        1 gcatcacgaa gacgtcgccg aggttgtaga tcgcgctgag gcgtccctcc tcgacggggc
       61 cgaggtagag gccgccgacc tcctcgatgc gcccgcccca gccgtccgga tcctcccggc
      121 ggccgaactg cccgatcgga cccgcgatca cgagctgcac gtcgcggcgc cgctcgcgga
      181 gcgcccgggc cgcccgcagc agcacgtcgc tccccttctg ctcgcagacc cggccgacgt
      241 agagcatcac cctcttgtcg atgccgagcc gggcccgctc gcgcgcggcc gcctccggat
      301 cgggtcggaa ctgccgggtg ttgaccccgt tgtggaggat ccgcagcttg ctcgcgggaa
      361 agctccagaa gctctgcgac tcctccaggc aatagcggga gcacggcagg agcaggtcgt
      421 aggagcggag cgcccgcttg tacaggtgat agagcggcgt cgcccggccg ccgcggaact
      481 ggaagaagtc gtacgagagc accttccggg ctcccaccgc ctggctcatc cacgcgccct
      541 cgggctgact gtggaagtgc agcacgtcgt cggagcgcct gctcagcgcg cgcacgacgt
      601 gatgctggaa ctccagatgc ctgaacggta agcgcgtccg gcacttcaag aagtgatagg
      661 tcacgccgtt caggtcgcgc gtctcggtga catcgccgac cgagtacacg ctgacccggt
      721 gcccgcgccg ggcctgctcg tgcgcgatct cgacgatgcg ccgctcgatg gcgccgccga
      781 agcgatgcag aaccggcagc gtgctgacgc tgatgtggct gatgttcatc gcggtatcgt
      841 cctcgccaga ctggggcggc ccgtccgccc cgcggcccgg ccgcggcgcc agtacacctc
      901 gaccgccagg cgcatcgccg gatcgatcca ggcggcccgc cgccgcgccg gcgcgacgcg
      961 gccctcgatc atgctccgat agagggcgag gtggcgggcg accatggtct ccggcttgaa
     1021 cgtgtgctcc gcgtatgccc gcatctcggc cgccatgtcc tgccagcgcg gccgctcggc
     1081 caggaccctc tccatcgcat ccgcgagggc gacggaatcg cccggcggca ccgccaggcc
     1141 gtagggcccg atctgctccg gcacgccgca caccccgccc gccaccaccg gcgtgccgca
     1201 caggaacgcc tcggtgatca cgctcggcag gcaatcggcg aagctcggca gcaccagcag
     1261 gtccgcctgg tgatactcct cggccagcgc ccccggatcg aggatgccca cgaactccac
     1321 ctgcttggcg atacccaggt cgtcggcctg ccgcctcagc tccgcctcca gctgcgcgtt
     1381 gtgatacacc agacggagat gcacgtcgcg ctgcttgcga agctgctgca tcgcttcgag
     1441 caggaagttg acccccttcc agtcgatcag ctggccgacg tagagcacgt cgtagcgggc
     1501 cttgtcggct cgcttccgcc gatgccccgc ggcgcggaag acgtcggccg gcacaccgtt
     1561 gggaatggtg tggatctttt cggcgctcgt cacgcgcgcg tagtaccggg cctcggcgcg
     1621 cgacagcgcg atgtaggcct ccgccctgtc caccacgtac tggaaggcgt agcgtcgtag
     1681 ccgcgagcgc tcccagccgg tggggatcag cccgttgtgg ccggtgaaga cgacgcgcgc
     1741 ccggccgcgc gacgccgcca tcttgagcgc ggcccgaccg aagtggtgga cgtgaatgat
     1801 gtcgtactcg tccgacggct gaaagccggc gtgtccgacc gcgtgcaccg tcacccccgc
     1861 cttgcggaag ccgtccagca acaccgtctc cggcgtcacc aactgccgcg atcggatcgg
     1921 ctcgggccga tcgaagatgc cgcccaccag acacacgttc atgcgaatcg ccgatcgcgg
     1981 tggccggcca tcgcggcatt ccacgaccag aacctccggg ccgcgaagta gacgtagatc
     2041 ggccagaacg agtacacata gatctggctc gtgaccgaca ggctccccgg gaacacggtc
     2101 cacagcgcca tcatgagcca ggcgtagctc tccaccgggt tcggggcggc cacgaacttc
     2161 ccgtgcagcc aggcgagaag agagccgagc agcacgccgc cgagcaccac gcccgcgaga
     2221 tggaagttga tgtacagctc gccggccagg ggaatgatct ggtcgatact gtccgtgtcg
     2281 ccgtagatga tgtgattgaa tatcacgggg ccactgatgt cgcgatacgg cttgcccagc
     2341 accggaatcg ggtagatcag cgacggcaac aaggtcttgc cgtagtagag cttgtcctcg
     2401 agtgactcga tcaggtaggc ggtcatctgc ggtcccgacg cgtagatctg cacgaactcg
     2461 accacgccct cggcgttcca cgtggccgcg acggccgcgg ccgacagctc ggtgatatcg
     2521 aggtcggtgg accgatacca gccgaacacg aacgccgccg acagcgcgag catgccggcc
     2581 aggaccacac ccttgaacgg aatgcgccac gcgtgcaccg agaacgcggc gcccagggcc
     2641 acgatcgggc caagcatcgt gccccggttg tagttgaaat tgaccaccag cagccccagc
     2701 acgaccccgg cggtcacccc gacgatcgcc ggcttgcgcc gcggcgcctt gccgagccac
     2761 cagctccacg ccagcaccac cgcgaagccg agaaagtgct tcaggaaggt gccggcggcc
     2821 ccctgcggtg tcgtcgcctc ctccgcccgc aggcgagcct cgatcggcga cgagacgtag
     2881 tcgacgaacc ccccgaggcc gccgtgggcc agcaagaacc ccagcacgcc caggaccgcg
     2941 aacggcacga tcaagtaggc ggtgcccggc gtcgattcgc ggcccggtcc tggccccccc
     3001 gtccggcggc tcatgcactg gtacgccaca cagaaggaca ggtagctcac cgcatgaacc
     3061 acgatggcca gattgatcgc gcccttcgcc ggcagatgcg gcagcgcccc ctgcgtgaac
     3121 ccatagtagc cgaccatcac cgtcacgagg acgagctgga tccagtagta gccctgggcg
     3181 atgttcgccg ggcacagggg agcggtcatg cggatccggc acgcgggcaa gcacgcgtgc
     3241 acgaacatga acgcgaggat gccgacgacc gcgagcggat actcctccgc caccggcgcg
     3301 cacgcgaacc cgatcagggc gacggcgttg atcgccagca cccagaacgt ggcgccgggc
     3361 atcgggctca ccgcactggc cctcatcgca cgctccggcg cgctctcatg aagtggatcg
     3421 tcgtcgcggc cagggcggcc gcggtcaccg caaaggcgat caggctgccc agcgccatcc
     3481 cctgctcgcg atagatcgga atcagctgcc acgacgcgat ggcaagcacc accgcgagca
     3541 gcacgtcgag gacgaagcgc cccatgacgg ccccctggct caccagcacc tgccccagca
     3601 ggttgttgag caccaccgcc accgacgagg cggacaggat gaccagcgtc atcccgccgg
     3661 cgcgatacgc gtcaccgaag aggcccatcg ccggcgccgc gcacagcatg actccgatcg
     3721 acgggaccac cacgaccgcg acgctgaccg ccaggttgag tccgaagagc cggcgatacg
     3781 cgtcgggatc gctccggccg tgcaggttgc tcagcatggt gaggatcacc ggcgagaacg
     3841 aagcgggaag gaagagcagc agctgccgcc agcgctcggc cgcgttgaag acgccgagcg
     3901 aggcgtaccc gtcgggctgc gcgaccagca ccaggttggc gaaccacatc gcggacatcg
     3961 tggcgatgct ccccagcagg ctcagggcgc tgaaccgccc catctgccgc agctcgttcc
     4021 acgccgcgcg gcgatcgtgc ccggcgacgg actcggggag caggcgccgc agcgcgacct
     4081 ggttggcgag caccgcgatc gcttcggtca ggaccaggcc gaccacgccg cccaggaccc
     4141 cgccgagctc gatgccgatg agcaagaatc cgcacaggca caccccgcgg acgcctgtca
     4201 ggaccgccac gatccgaaag tcgccgaggc ccacgagggt gccggtctgc acgccgttga
     4261 tcgtgccgaa gaggaccagc ccgctcgcta cttgcagctc cgcggtcagg tggggggccc
     4321 tcagcacgcc cgacgccatc gctcccgaaa gagcgaggag ccccagcgcc atcaccgcgc
     4381 ccgagatcag ggcgatggtg gtggccaggg tggcgcagcg gcgggtcctc gcgggatcgg
     4441 tcgagcgaaa ctccgcgacg aacttggtcg cggccagccc gagcccggcc ccggccacga
     4501 tgccgaacag cccctgcgtg ctctgaatca tcccgatctc gccgaagccc gtcgtgccca
     4561 ggagccgccc cgccaccacc gaggacgcca gcgtcaagcc gcgcgacacg atcgcgccca
     4621 ccagcgccca gaacgcgccg atcgcgaaat gccgggcgac cgaatcgccc ggggcggcag
     4681 acatcgccgg ctccctcttg gttattgcta tcgcgcccgg caggccttcc atcatccgct
     4741 cacggttctc ggcaaccggc gtagaggtgc gtccctggat caactggctt cgcgctcgga
     4801 aggtacggac gccaccgccg ccttccttct cgtgtcaggg tgaagcccca cgtcatccac
     4861 ctcctcgagc ggctgttcga gcctcacccc gctggatcgc accaccttca acacggtgcg
     4921 acggaggatc ttcaggtcca gggcgaggct ccagtggtcc acgtaccata cgtcgagctt
     4981 gaggcggtcg ctgaagacga ccgcgttccg cccgtgaatt tgcgcccacc cggtgatgcc
     5041 gggcagcacc tcatgccggc gctgctgctc cggcgtgtag ttgggaacgt attccatcag
     5101 cagcggccgc ggcccgacga gcgacatctc gccgcggacc acattccaca actgcggcag
     5161 ctcgtcgagg ctcgtcttgc gcaagtaccg gccgacgcgc gacaggcgct cagggtcggg
     5221 cttgaggcgg ccgtcggctt cccgctcttc ggtcatggtg cggaacttca gcaactcgaa
     5281 cggtcgtccg tgcaacccgg gacgctgctg acgaaacagc accggccgcc caagacccgc
     5341 catgacgagc gccgcaacca gtgcatacaa gggcgacaag agaacaagca ccacaacgga
     5401 cactgctaca tcgagcagtc gcttgcccgg ctgcactcgc cgggtcatga cgaaaaaggc
     5461 tccgcccgga ccgccccgat gcgttcgtgg tccgggaccg ccgccggcga catgaccgcc
     5521 cgatctcccg cgatacgccg ggccagcgca cgaccgctga acgctgtccc cgatacgaac
     5581 cggcacagtg gtccgtacga ccacgccgcg gccgccccga ggaaatgcag gcccgccagc
     5641 gacgattcaa agccgtcggt cagaagcgga tagccgtcgg cggttcgcac gctcgacaga
     5701 agatccggtg tgaggaagcc gtaccgtgca atgtccaccc gatagcccgt gcagatcagc
     5761 acatgatcgc tgttgcgccg gctgccatcg tcgagcctca cttcgacacc gtcctcggcc
     5821 cgcctcgccg cctcgacgac tcgcgaggtc ctgatcggga catcgtggag ccgaccggcc
     5881 agccagcccg cgcccgcagg ccgaatcgag cgaaaggcga tccgctctct catggcgcgc
     5941 ggcagccgcc ggaacacgtc gggcgccgcc acgatccggt tgagaatcgg cgggccgacg
     6001 tcggtcggcg gatacagcag cgaccgaagc ggattccggt ggctcttgag ccaacccgcg
     6061 cgctggtcga gccagcgaat tctttcctgc cgcgctatga cctccacctc cgctcccgcc
     6121 tcctgcagca acgccgcgga ttcaagtgcg ctctggcctc cgccaacaac gacgacgcgt
     6181 ctccccgaaa agtgacggag atcacgatgc tccccggagt gcgaagccag ctccttcgaa
     6241 agaccatcga actggggagg tcgccaccca aaccgtgaaa ttcccgccgc gaccaccacg
     6301 cgccgcgcga gcacggcgtc accgtcatcg agccggaggc gaaatccctt cgcttcccgc
     6361 tcgatccgaa ccacccgtcg gcgatccaca tccggcgcga cgtgccgctg gaaccatcga
     6421 ccatagccga caaagcgatc cagcggaatc ggtttcgaga tactcgacct ctcactcgcc
     6481 gaaaactcgt cgagcgtcag ctcgcgcctg gggtggccga tatgcgacgc ggcccacgag
     6541 gaccgcagac acatccccat cggcatctgc gtttcccaga actccatcgt ctcgccgaag
     6601 acgcacgttt cgacgccggc cgcgcgaagg tgagccgcgg ccgccaagcc gtatggtccg
     6661 gcaccgatga tcgccacatt gacgctcggc tcagtcttca tcgcgcgctt cgggcgcgcc
     6721 actacgactt ccattggact gtcctgcgtg ggttctgggt gctggcgagc gaggcggctc
     6781 ttcccgaatc cgccacccgg cgtgtgacga cgaagctgtc gcttcgcgcc gccgtaaccc
     6841 cgggcgcgca gtcgaaacaa ctcgcctccg gctcggcgcc ggccgcccac gcgacgagcg
     6901 cggccgggac attcgccccg gccacatgcg aaaaaggata gcccccgccg aaccgcggat
     6961 tgaggtcgat gacatggcat ccggccgcgg tcaccaacac gtcgcaatcg aggacaccga
     7021 catgcccgag gctctcgcca atcgttcgcc cgagttcttc gagccgggaa tcctgaaccg
     7081 tcaccgcgcg atcgttctgg cccgcccgca tccgcagctt acgcttgatg aaggtgcaca
     7141 cgtggcggcc ttcgagatcg ttcacgatat cgagaccgaa ttcaacgccg tcaaggcact
     7201 cctgaatcag cacacaccgc tcaggatccg tggcgctgac ctcggcgaag gagctgcgag
     7261 cgacgtgctt cgcggccagc cgaaagcata gccccagctc ttcgtcatcc tccgcgacgg
     7321 cgagcccgat cgaactgacc ccccagcgcg gcttgaccac cagcggcaac cggagttcgc
     7381 cccgggccag agccgcgctc gctgcgtcca gggaaagcca cgttgccggc accggtagat
     7441 tcagcgcggc gagaaaccgc gccgtctcga gcttgtcgta acacgtcgcg acgactttgc
     7501 tcgaggacac aagcgccacc gttcccgcct cgcgaaatcg cgatcgagcc tctgccagca
     7561 ggggcaattc aggctccagc gtcggaacca gcaggccgac gcgccgttca cggcagatgg
     7621 cgagcagcgc gtccaggtag cccgctgcgc caaggagggg aacgaggaat gaatcgtccg
     7681 cctcctggag cgccggcgca tctccgctcg agtcacacgc aaacaccagg ccgcggccac
     7741 gcaccgcttg tttgaaggcg gcgatcgtat gactgcgacg cccggcgcac gtcac
//
LOCUS       DBNGZW010000582         8719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_805464, whole genome shotgun sequence.
ACCESSION   DBNGZW010000582 DBNGZW010000000
VERSION     DBNGZW010000582.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8719
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_805464"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1917)
                     /locus_tag="AC16JC_13750"
     CDS             complement(<1..1917)
                     /locus_tag="AC16JC_13750"
                     /inference="COORDINATES: protein motif:HMM:NF021942.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HIR5165808.1"
                     /translation="MHRQRSMVLLVALFGLLPSPASLIAQQAPAAGGEYPGLETGTMW
                     TFDVPPLDYWAKRYNFRPAAGWLDHVRLSAARIPGCSASFVSPDGLVMTNHHCARACI
                     DAVTKPGEDLLANGFTAATHAEERPCPGMVLDQLQAISDVTDSVTGAVPGDAPASRAA
                     RLRSEAIAGIERRCNASASDANCQVVTMYRGGQYKLYRFRRFNDLRLVFAVEAQTAFF
                     GGDPDNFTYPRYDLDVSMVRAYVDSQPAHTEYLRWSTRGASEGDLVFEVGNPGSTGRL
                     NTVAQLQYLRDVQYPATLRLLADEIRVYQTLAAADSDRASALRNTLFGLENSQKAIRG
                     YHSGLLDPQVMARKREWERAFRTKVDTDPVRRRLYGDAWDQTAAIRRQLAGLDVRRRY
                     HAFGAYGSRLLGLAGMLTRMPAESAKPDSARLAMFRDANRSRLERVLYAPQPVDTAVE
                     AQLLAAYFGAMQKELPATDPVLVAALGGRSPEQAAHRMVAATSLGSAEGRRALAQGGS
                     AALAASRDPFIALARVIDPLERRMQRQLDSLLDLESRNDERVARALLAVFGSSVAPDA
                     TFSLRISDGSVLRYPMNGTFAPAFTTLYGLYDRAAGFGGKEPWNLTPRWQAARDSLDL
                     ATPLNGVCTCDIIGG"
     gene            complement(1959..3191)
                     /locus_tag="AC16JC_13755"
     CDS             complement(1959..3191)
                     /locus_tag="AC16JC_13755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HIR5165809.1"
                     /translation="MVAPPRRILLVDADAFYVAVARLVDPEGAGRARLLIVGGSPERR
                     GVVTSASYEARAFGVHSAMPMARAVRLCPGATVVAVPWEACAAKGRAIGDVLERFTPA
                     VEQASSDEFYLDLSGTERLYHDEPLAATAGRIRAAVREATSLWMSIGGGTSKLVAKLA
                     AGLAKPAADVPGAGVHVVEPGGEAAFLAGFALADIPLIGPKTQDRLARLGWRTAADVQ
                     RLERSELVRRLGEREGEWLWERVRGLDDSTVEPWTDPKSISRDNTFPTDLDADDDLLR
                     ELLIQVDRATADLRAEGLVARTITVRLRDADFTTRQAGRTLPEPVLTDRAVYDVAREL
                     LAKLRRARRVPARLIGVALSQLDRPGAAAQLALLEAERTDVPETEKDRQLARAMDAVR
                     DKFGPDALGRARTQEWKG"
     gene            3252..4025
                     /locus_tag="AC16JC_13760"
     CDS             3252..4025
                     /locus_tag="AC16JC_13760"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR5165810.1"
                     /translation="MPRLSKWTGLIIVANIAAYLWSASFPEQAEAFVFRPVLFLARPW
                     TAVTYMFLHQGFGHIFFNMLVLFFFGPQVEDRLGSGRYLTLYFVSGLFGAATSMLFNP
                     LASIVGASAAVYGVELAFAYFWPRAQLLIWGIVPVEARTLIIIMTIISVMGGVGWGMQ
                     NVAHFAHLGGFLGGWLVLKVFGVRRFEDKLRPRQSRAESGSTRLAPGAAERWKQIPLD
                     RLHPVNREEAERVLAKLDARGPTGLTAEEQAFLDRFTSM"
     gene            complement(4012..4431)
                     /locus_tag="AC16JC_13765"
     CDS             complement(4012..4431)
                     /locus_tag="AC16JC_13765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HIR5165811.1"
                     /translation="MSYWILKTEPSTYSFADLQKSKTTVWDGVKNPLALKHLRAMQVG
                     DEVLIYHSGSEKAAVGTARVTKAAYQDPKKSDPKLVVVELRAGTALPAAVPLSMIKQD
                     AAFKELALVRMPRLSVVPATAPQWKRLLALAATATSK"
     gene            complement(4519..5196)
                     /locus_tag="AC16JC_13770"
     CDS             complement(4519..5196)
                     /locus_tag="AC16JC_13770"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5165812.1"
                     /translation="MTESQPLRITLPGSGTPVSGLLLQPPDARLLYVLAHGAGAGMRH
                     PFLERTAAALAERGIATLRYQFPYMERKQRRPDPPAVAAAAVRAAVVAAAAAPHLPLI
                     AGGKSFGGRMTSTAQAEQPLPGVRGLAFLGFPLHPPGKPAVSRADHLDDVAIPMLFLQ
                     GERDEFAEPLLLRQVLRRLGERATLHLVKGGDHSLSVPKSSGRIPDQVLAEVCDALAA
                     WALDLEP"
     gene            complement(5200..7779)
                     /gene="ligD"
                     /locus_tag="AC16JC_13775"
     CDS             complement(5200..7779)
                     /gene="ligD"
                     /locus_tag="AC16JC_13775"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA ligase D"
                     /protein_id="HIR5165813.1"
                     /translation="MRGHKRSTIESVSGNRKPTDDALSTYRAKRSADRTPEPFGTVAA
                     SIGAGRFIVHQHAATRLHFDLRLEMDGVLKSWAVPKGPSRNPADKRLAVHVEDHPIEY
                     GEFEGIIPEGNYGAGAVIVWDRGVWVPLEDPVEGLKKGKLLFELRGYKLKGVWTLVKI
                     KKGVKEWLLIKERDGYVSTNGDQFPPGSVLSGLTVEELRDGESKTAKLVAELKRLKAP
                     RRAVRAEDVPPMLAETREEPFSRAGWLFEVKLDGYRMRIAREGGKARLITRNGNDYTA
                     AFPELERAVRALPYEGLVMDGELVILDEQGRPSFQRLQNRARVGRAPDIRHAAVENPA
                     TVYVFDLMALEGFDLRPLPLVKRKAILKKLLPEAGPLRFSEHFEKNGEALYDQAVGLG
                     LEGIVAKKADAPYTSGRSDLWLKVRADKTGDFVVVGYTEPRGSRGGFGALHLGAYRDG
                     TLVYIGRAGSGFSAKQLKEVSADLEGLRLDKPSFAGPVPAERESRWVKPELVAEVRYK
                     EITGDGLLRQPVFLRFRDDKKAEECVIGGPGTGDEGRVVDEPAPLRTRPASPVPRPSV
                     QFSNLDKIFWPEEGYTKGDLIEYYRAVSGWMLPYLAERPLVLTRYPDGINGKSFFQKD
                     APEYAQAFVRTVTIWSEDSQRELDYFVCDTEDSLLYIANMAAILLHIWSSRVATLETP
                     DWCILDLDPKDAPFTDVVTVAHAVKDLCDDIGLPTGIKTSGSTGLHVLIPLGRQCTYE
                     QSRTLGGLLARVIASRLPEIATVTRQVQRRGGKVYLDYVQNGHGRLLVAPFSARPLRG
                     APVSTPLTWRDVTPRLDIRAFTIKTVPARMKRLKQDPLWAMLDQQPDLMSALSRLHQR
                     LDE"
     gene            7768..8640
                     /gene="ku"
                     /locus_tag="AC16JC_13780"
     CDS             7768..8640
                     /gene="ku"
                     /locus_tag="AC16JC_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011985253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end joining protein Ku"
                     /protein_id="HIR5165814.1"
                     /translation="MPPHAIGSATISFGLVSVPIKMFSAGESSAAISFNWLHKKDGSR
                     LKQQYICAKDGEKVEDADKVKGYEFAKGQYVIFTKDELKALEEKGTGSIDITEFVDAE
                     TVDRMYMDRVYFLGPEKGGDRAFALLGEALKKTRKVAIGQWAARGKQYLIMIRPLGPG
                     LAMEQLRYANEVRSIDEVPIPKVDVKKAEVDLAVQLIQQAASEAFQPTKYEDTVKKRI
                     LEQIERKVEGKEITEEPTEAPKTQIIDLMEALKASLAKGKGTAGAERKPAKRAEAAAK
                     PAKTRQVKRKAASA"
BASE COUNT         1365 a         3014 c         2989 g         1351 t
ORIGIN      
        1 gcccccgatg atgtcgcagg tgcagacgcc gttgagtggg gtcgccagat cgagggaatc
       61 gcgagcggcc tgccaccgcg gcgtgagatt ccaaggctct ttgccgccga agcccgcggc
      121 gcggtcgtag aggccataca gcgtcgtgaa cgccggggcg aaggtgccgt tcatggggta
      181 gcgcaacacc gagccgtcgg agatacgcag gctgaaggtg gcgtcgggag cgacgctcga
      241 gccgaacacg gcgagcagcg cgcgcgccac ccgttcgtcg ttgcggctct ccaggtcgag
      301 cagcgaatcg agctgccgct gcatcctgcg ctccaggggg tcgatcaccc gggccagagc
      361 gatgaaggga tcgcgtgacg ccgcgagcgc cgcgctcccg ccctgcgcga gcgcacgccg
      421 tccctcggcg gagccaagcg aggtcgccgc caccatgcga tgcgccgcct gctcggggct
      481 gcgcccgccg agcgcggcca cgagcacagg atcggtcgcc ggcagctcct tctgcatggc
      541 gccgaaatac gcggcgagca gctgcgcctc gactgccgta tccaccggct gtggcgcata
      601 gagaacgcgc tccaagcgac tgcggttggc gtcccggaac atcgcgagcc gcgcgctgtc
      661 gggcttggcg ctctccgcgg gcatccgcgt cagcatgccg gcgagcccca aaagccgcga
      721 tccgtaggcg ccgaacgcgt gatagcggcg gcggacgtcg agccccgcca gctgacgtcg
      781 gatggcggcg gtctggtccc aagcgtcgcc gtagaggcgg cgacgcaccg ggtcggtgtc
      841 cactttcgtc cggaaggcac gctcccactc ccgcttgcgg gccatcacct gggggtcgag
      901 cagccccgag tggtagccgc ggatcgcctt ctgggaattc tccagcccga acagcgtgtt
      961 ccgcaacgca ctggcgcggt cgctgtccgc ggccgcgagc gtctggtaca cccggatctc
     1021 atcggccagg agccggagcg tggccgggta ctgcacgtcg cgcaggtact ggagctgcgc
     1081 caccgtgttg agccgtcccg tcgagccggg attcccgacc tcgaagacca ggtccccctc
     1141 gctcgccccg cgggtgctcc agcgcagata ctcggtgtgc gccggctggc tgtccacgta
     1201 ggcccggacc atcgagacgt cgaggtcgta gcgcgggtag gtgaagttgt ccggatcgcc
     1261 accgaagaac gccgtctgcg cctcgaccgc gaacaccaac cggaggtcat tgaagcggcg
     1321 gaagcgatac agcttgtact ggccgccgcg atacatcgtg acgacctggc agttggcatc
     1381 ggacgcgctg gcgttgcagc gccgctcgat gccggcgatg gcctcgctcc gcagccgggc
     1441 ggcccgcgaa gccggggcgt caccgggcac ggccccagtg acgctgtcgg tcacgtcgct
     1501 gatcgcctgc agctggtcga gcaccatccc ggggcagggc cgctcttccg cgtgggtcgc
     1561 agcggtgaaa ccgttggcca gcaggtcctc tcccggtttg gtgacggcgt cgatgcaggc
     1621 gcgtgcgcaa tggtgattcg tcatcaccag gccgtcgggg gagacgaacg acgccgagca
     1681 gccggggatg cgggcggcgg agagccgcac gtgatcgagc caccccgcgg cagggcggaa
     1741 gttgtagcgc ttcgcccagt agtcgagggg gggaacgtcg aacgtccaca tcgtcccggt
     1801 ttcgagcccc ggatattcgc cgccggccgc cggcgcttgt tgggcgatga gcgacgcagg
     1861 gctcggcagg agcccgaaca gggcgacgag gaggaccatc gaacgctggc ggtgcatgat
     1921 gcactcccgg agggcagatg agtgtcgagg agacgaatct agcctttcca ttcctgtgtg
     1981 cgcgcgcgac ccagcgcgtc gggaccgaac ttgtcccgca ccgcgtccat tgcccgggcc
     2041 agctggcggt ccttctcggt ctccgggacg tcggtccgct ccgcctcgag cagcgcgagc
     2101 tgcgccgccg cgccgggccg gtcgagctgc gacagcgcca ccccgatgag ccgcgcggga
     2161 acccggcgtg ccctgcgcag cttggcgagc agctcgcgcg ccacatcgta gaccgcgcgg
     2221 tcggtcagca cgggctcggg cagcgtccgg cccgcctgcc tggtggtgaa gtccgcatcg
     2281 cgcagccgga cggtgatcgt gcgggcgacc agcccttccg cgcgcaggtc cgccgtcgcg
     2341 cggtccacct ggatgagcag ctcgcgcagc aggtcgtcgt cggcgtccag gtcggtgggg
     2401 aaggtgttgt cgcgactgat cgacttcggg tcggtccagg gctccaccgt cgagtcgtcc
     2461 agtccccgga cccgctccca cagccattcc ccttcgcgct cccccaaccg gcgcaccagc
     2521 tcggagcgct ccagccgctg cacgtcggcc gcggtgcgcc accccagccg cgccagccga
     2581 tcctgcgtct tggggccgat caggggaatg tccgcgagcg cgaagccggc gagaaacgcc
     2641 gcttcgccgc ccggctccac gacgtgcacg ccggcgccgg gaacgtccgc tgccggcttc
     2701 gcgagacccg ccgcgagctt ggcgaccagc ttcgacgtgc cgccaccgat ggacatccag
     2761 agcgatgtcg cctcgcgcac cgccgcgcgg atccgtcccg cggtcgcggc gagcggctcg
     2821 tcgtgataca gccgctcggt gccggagaga tcaaggtaga actcgtcgct cgatgcctgc
     2881 tccacggccg gcgtaaaccg ctccagcaca tccccgatcg cccggccctt ggccgcgcac
     2941 gcctcccacg gcacggcgac gacggtcgcg cccgggcaaa ggcggacggc acgcgccatc
     3001 ggcatcgccg agtgcacgcc gaacgcgcgc gcttcgtagg acgccgaggt cacgacgccg
     3061 cgccgttcgg gcgagccccc cacgatcagg agacgggccc ggcccgcacc ctccggatcg
     3121 acgaggcgcg cgacggcgac gtagaaggcg tcggcatcga cgagcagaat gcgacggggc
     3181 ggagcgacca tggccaaggc gggaacttaa ctcttgctca ccgtccggcg ccgggtatag
     3241 ccttccctgc catgccgcgg ctttcgaagt ggaccggcct catcatcgtc gcgaacatcg
     3301 cggcctatct ctggagcgcg agcttccccg agcaggccga ggccttcgtg ttcagacccg
     3361 tgctgtttct ggcgcggccc tggacggcgg tcacctacat gtttctccat cagggcttcg
     3421 gccacatctt cttcaacatg ctggtgctgt tcttcttcgg gccgcaggtc gaggaccggc
     3481 tggggagcgg acgctacctc accctgtatt tcgtcagcgg ccttttcggt gcggccacgt
     3541 ccatgctgtt caacccgctc gccagcatcg tcggagcgtc ggcggcggtg tacggagtgg
     3601 agctggcgtt cgcctacttc tggccacggg cgcagctgct catctggggg atcgtgccgg
     3661 tcgaggcccg cacgctgatc atcatcatga cgatcatctc ggtcatgggc ggcgtcggct
     3721 gggggatgca gaacgtggcg cacttcgccc acctcggggg atttctggga gggtggctgg
     3781 tactgaaggt gttcggggtg cggcggttcg aggacaagct gcggccgcgc cagtcgcgtg
     3841 cggagtcggg cagtacgcgc ctcgcgcccg gtgcggcgga gcggtggaag cagatcccgc
     3901 tcgaccggct ccacccggtg aaccgcgagg aagccgagcg cgtgctcgcc aagctcgacg
     3961 cgcgcgggcc caccgggttg accgccgagg agcaggcgtt cctcgaccgc tttacttcga
     4021 tgtagcggtc gcggcgagcg ccagcagccg tttccattgg ggcgcggtgg cgggcacaac
     4081 cgacagccgc ggcatccgca ccagtgcgag ttccttgaac gcggcatcct gcttgatcat
     4141 cgacagcggg accgcagcgg gaagcgcggt tccggcccgc aactccacca cgacgagttt
     4201 cggatccgac ttcttggggt cctggtaggc ggccttcgtg acccgagccg tgcccaccgc
     4261 ggctttttcg ctccccgagt ggtagatcag cacctcgtcg cccacctgca tcgcgcgcag
     4321 gtgtttcagc gcgagcgggt tcttcacgcc gtcccagacg gtggtcttcg acttctgcag
     4381 atccgcgaag ctgtaggtgg acggctcggt tttcaggatc cagtagctca tgattccggt
     4441 ctccttggag tcgccgtgac aggccgggga ctcactccgc gacaaaacga cgcagtcgct
     4501 ccgtgagtcc ccggcccgtc acggctcgag atccagcgcc catgccgcga gggcatcgca
     4561 gacctccgca agtacctggt cgggtatccg tcccgagctc ttcggcacac tgagggagtg
     4621 gtccccgcct ttcaccagat gcagcgtggc tcgctcaccc aggcgccgca agacctgccg
     4681 cagcagcagc ggctccgcga actcgtcccg ttccccctgc aggaagagca tcgggatcgc
     4741 gacatcgtcc aggtgatcgg cgcgagacac ggcgggcttg cccggtgggt gcagcgggaa
     4801 acccaggaag gccagtccac gcacgcccgg gagcggctgc tccgcctggg ccgtggaggt
     4861 catgcgcccg ccgaacgact taccgccggc gatgagcggt agatggggcg ccgccgccgc
     4921 cgccacgacc gccgcccgca ccgcggcggc cgccaccgcc ggcggatcgg gacggcgctg
     4981 cttgcgctcc atgtagggaa actgatagcg cagcgtcgcg atgccgcgct ccgcgagcgc
     5041 cgcggccgtg cgctcgagaa acgggtggcg catccccgcc cctgcgccgt gcgccaggac
     5101 gtagagcagg cgcgcgtcgg gcggctgcag cagcagcccg gagaccgggg tgccggaccc
     5161 cggcagggtg atgcgcagtg gctgcgactc ggtcatcggc tactcgtcga ggcgctggtg
     5221 gagacgactc agggcggaca tcaagtccgg ctgttgatcg agcatcgccc agagcgggtc
     5281 ctgcttgagg cgcttcatgc gcgccggcac agtcttgatg gtgaaggcgc ggatgtcgag
     5341 ccgcggcgtc acatcgcgcc aggtgagcgg cgtcgagacc ggcgcgccgc gcagggggcg
     5401 cgcgctgaac ggtgccacca gcaggcgccc gtgcccgttc tggacgtaat cgaggtagac
     5461 cttgccgccg cgccgttgca cctggcgcgt cacggtcgcg atctcgggaa gccggctcgc
     5521 gatgacgcgc gccagcaggc caccgagggt gcgcgactgc tcgtaggtgc actggcggcc
     5581 cagcggaatg agcacgtgca gcccggtcga gccgctcgtc ttgatccccg tgggcaggcc
     5641 gatgtcgtcg cacaggtcct tcacggcatg ggcgaccgtg acgacgtcgg tgaagggcgc
     5701 gtccttgggg tcgagatcga ggatgcacca gtcgggggtt tccagcgtcg cgacccgcga
     5761 cgaccagatg tgcagcaaga tggccgccat gttggcgatg tagagcagcg aatcttcggt
     5821 gtcacacacg aaatagtcca gctcgcgctg cgagtcctcg ctccagatcg tcaccgtacg
     5881 gacgaacgcc tgggcgtact ccggagcgtc cttctggaag aacgacttgc cgttgatgcc
     5941 gtcgggatag cgcgtcagga cgagggggcg ttcggcgagg tagggcaaca tccagcccga
     6001 gacggcccgg tagtattcga tcaggtcgcc cttggtgtag ccctcctcgg gccagaagat
     6061 cttgtcgagg ttggagaact gaaccgaggg acgagggacg ggggacgcgg gacgagtgcg
     6121 caacggggcc ggctcgtcca ccactcgtcc ctcgtccccc gtccccggtc ccccaatcac
     6181 gcactcctcc gccttcttgt cgtcccggaa ccgcaggaag accggctgcc gcagcaatcc
     6241 gtccccggta atctccttgt agcgcacctc ggccacgagc tccggtttca cccaccggct
     6301 ctctcgctcc gcgggcaccg gtcccgcgaa ggacggcttg tccaggcgca gcccctccag
     6361 gtcggccgag acttccttga gctgtttcgc cgagaagccg ctgccggcgc gcccgatata
     6421 gacgagcgta ccgtcgcggt aggcgccgag gtgcagcgcc ccgaacccgc cgcggctccc
     6481 cctgggctcc gtgtagccca ccaccacgaa gtcgccggtc ttgtccgcgc ggaccttgag
     6541 ccagaggtcg gagcggccgc tcgtgtacgg cgcgtcggct ttcttggcga cgatgccctc
     6601 gagccccagg cccacggcct ggtcgtagag cgcctccccg ttcttctcga agtgctccga
     6661 gaatcgcagg ggcccggctt ccggcaacag cttcttgagg atcgccttgc gcttgacgag
     6721 cggcagcggc cgcaggtcga acccctcgag cgccatcagg tcgaagacat agaccgtcgc
     6781 cgggttctcc accgccgcgt ggcggatgtc gggcgcgcgg cccacccgcg cgcggttctg
     6841 caggcgctgg aagctcggcc gtccctgctc gtccaggatg acgagctcgc cgtccatcac
     6901 gagtccctcg tagggcagcg cgcgtaccgc gcgctccagc tcgggaaacg ccgcggtgta
     6961 gtcgttgccg ttacgcgtga tgaggcgcgc cttccctcct tcacgcgcga tccgcatgcg
     7021 gtaaccgtcg agcttcacct cgaacagcca cccggcgcgc gagaacggct cctcgcgcgt
     7081 ctcggcgagc atcggcggca cgtcctcggc gcgcaccgcg cggcgtggcg ccttgagccg
     7141 cttcagctcg gccacgagct tcgcggtctt gctctcgccg tcgcgcagct cctcgaccgt
     7201 cagccccgag agcaccgacc ccgggggaaa ttgatcgccg ttggtcgaga cgtagccgtc
     7261 gcgctccttg atcagcagcc attccttgac gcccttcttg atcttcacca gcgtccagac
     7321 gcccttcagc ttgtagccgc gcagctcgaa cagcagcttg cccttcttca gcccctcgac
     7381 cggatcctcg agcggcaccc acaccccccg gtcccagacg atgaccgcac cggcgccgta
     7441 gttgccctcg ggaatgatgc cttcgaattc gccgtattcg atcgggtggt cctccacgtg
     7501 cacggcgagg cgcttgtctg caggattgcg cgagggtcct ttgggcaccg cccaggactt
     7561 gagcaccccg tccatctcga ggcgcaggtc gaagtggagt cgagtagccg cgtgctggtg
     7621 tactatgaag cgccccgcgc cgatgctggc ggcgacggta ccgaacggtt ccggggtgcg
     7681 atccgcggag cgtttggcgc ggtaagtgct tagcgcgtcg tcggttggct tgcggtttcc
     7741 actcacggat tctatagtag acctcttatg ccccctcatg caatcggctc cgccacgatc
     7801 tctttcggcc tcgtttccgt gccgatcaag atgttctcgg ccggtgaatc ctcggccgcg
     7861 atcagcttca actggctcca caagaaagac gggtcccgcc tgaagcagca atacatctgc
     7921 gccaaggacg gggagaaggt ggaggacgcc gacaaggtca agggctacga gttcgccaag
     7981 ggccagtacg tcatcttcac caaggacgag ctgaaggcgc tcgaggagaa gggcacgggc
     8041 tcgatcgata tcacggagtt cgtggatgcc gagacggtcg accgcatgta catggaccgg
     8101 gtctacttcc tgggacccga gaagggcggc gaccgcgcct tcgcgctgct cggcgaggcg
     8161 ctgaagaaga cccgcaaggt agcaatcggc cagtgggcgg cgcgcggcaa gcagtatctc
     8221 atcatgatcc gcccgctcgg ccccgggctc gcgatggagc agctgcgcta cgccaacgag
     8281 gtgcgctcga tcgacgaggt gccgattccc aaggtcgacg tcaagaaggc cgaggtcgac
     8341 ctggccgtgc agctcatcca gcaggccgcg tccgaggcgt tccagccgac caagtacgag
     8401 gacaccgtca agaagcggat cctggagcag atcgagcgca aggtcgaggg caaggagatc
     8461 accgaggagc cgaccgaggc gcccaagacc cagatcatcg atctgatgga agcgctcaag
     8521 gcaagcctcg ccaagggcaa gggcacggcc ggggccgagc gcaagccggc gaagcgcgcc
     8581 gaggccgcgg cgaagcccgc gaagacacgc caggtgaagc gcaaggcggc cagcgcctag
     8641 ggcgatgacg ggatacacca ccgaggaggt cgcgcgtctc ctcggtcttt cctccgcgca
     8701 gatccgatcc tacactcga
//
LOCUS       DBNGZW010000583         4403 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_939190, whole genome shotgun sequence.
ACCESSION   DBNGZW010000583 DBNGZW010000000
VERSION     DBNGZW010000583.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_939190"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1237
                     /locus_tag="AC16JC_13785"
     CDS             143..1237
                     /locus_tag="AC16JC_13785"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5165815.1"
                     /translation="MRFVRSTGIRIVAAWLAVAVFFSAENILVGTARHRPFTWQWDVY
                     HEFVYWLVWAAFTPIILGAGRRWPLGPGALPRVIAPHLGVMALLAPAQITTTYTVHWL
                     LLSAVGRPPAATLGAWLGGLGGGIVWGTLTAFLYYWLILGVHAAFVYQRMYQAQRVAT
                     AELEGRLAQAQLETLRLQLHPHFLFNTLNAIAAFVGSEPDRARRMIARLGELLRRTLD
                     SGTAAEVPLARELELLAPYLEIQRIRLGERLHVDVDIPEAARTALVPTLLLQPLVENA
                     VEHGVSRQPGSALVRLHAVRAGARLRLEITDDGPGPPAAGGDDGIGLANTRERLVRLY
                     GAAHRFELSPAGAVGTTVAIEIPLRTAEGA"
     gene            1239..1982
                     /locus_tag="AC16JC_13790"
     CDS             1239..1982
                     /locus_tag="AC16JC_13790"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytR/AlgR family response regulator
                     transcription factor"
                     /protein_id="HIR5165816.1"
                     /translation="MRVVIVDDEPPGRLALREQLAAMPDAEIVAECENGAAAIEAIVR
                     HAPDLLLLDVQMPGLGGFDVLERVGADAVPAVVFVTAYDRYAVRAFEAHAVDYLLKPI
                     DPDRFREAYQRAARRVERDGGEHASRLEKLVAGDAPAPLDRFVVKDKGRLVFVGVETV
                     DWIEAAGNYVTLHAGPAVYGLRRTLASLAMRLGERFVRIRRSALVNVHAIRALEQYGK
                     GSYVVYLRDGTKLISSRYAGRELRALLDT"
     gene            complement(1990..2208)
                     /locus_tag="AC16JC_13795"
     CDS             complement(1990..2208)
                     /locus_tag="AC16JC_13795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165817.1"
                     /translation="MRPVSIAGLLLLAAGLFVALRGVSYRSSRSDLRVGDFQASLEER
                     RTIPPWVGWVGAGLGLVLVVAGRSRRGS"
     gene            complement(2311..3738)
                     /locus_tag="AC16JC_13800"
     CDS             complement(2311..3738)
                     /locus_tag="AC16JC_13800"
                     /EC_number="4.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016573976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016830 - carbon-carbon lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR5165818.1"
                     /translation="MTYSVLSSDRQQLAALLGGAKDDAVRFLGSLDERPVAHVVPAIA
                     PLGLPTHGFGTAGALEAFRQRYAPWLSASAGPRYFAFVTGGSTPAALVGDWLTSVYDQ
                     NGSDAGESSARHIALDALAMVRDLVGLPDGFQGAFVTGATTSAIVALATARQWVGRSR
                     GVDASVEGAHALGPVRILSGTAHSSISKAASVIGLGRTALHGVATLPSREAVDVEALA
                     ASLQAAEREGVDPVIVVANAGTVNTCDFDDLRAIDALKSRHSFWLHVDGAFGAIAAAS
                     PRYRPLLDGLEAADSVSVDGHKWLNVPYDNAVILTRHLGLQGETFQSSGAYLPAEVRP
                     DTLVHLTPENSQRLRALPVWMTLAAYGRAGYAEIVERCCAHAAWLGARIEEHPGFRLL
                     APVKLNGICFTVARDGGDRASAEEVTEFLERLKGGGVTFLTPTILRGVPAARVSISNW
                     RTDHADMERTWGAIQEAVRRRPSRG"
     gene            3902..>4403
                     /locus_tag="AC16JC_13805"
     CDS             3902..>4403
                     /locus_tag="AC16JC_13805"
                     /inference="COORDINATES: protein motif:HMM:NF047071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5165819.1"
                     /translation="MPPALLSRAEVIERLMLVIRRFGYDGASLAELSKATGLGKSSLY
                     HYFPAGKDDMVRAVLEHFESQLQELVFTPLRTPGPARRRVEGMVRTLDAFYRGGREAC
                     VLGNLVLGTSRTRFRSRLRAIFDRWIDALAVALADSGLPKSAARARAEDAVMRIEGAL
                     ILAGGMD"
BASE COUNT          654 a         1549 c         1542 g          658 t
ORIGIN      
        1 agttgggaac agcggcacgc tgcgccaagg acgagggggc cgctgcggcc cgggcggtga
       61 cgaatgcggc cggaaccggg acgagtggtc cagccccgcg cgggccgttg cccgggacga
      121 gccgtgcggc gaagattgcg tcatgcgatt tgttcgatcg accggcatca ggatcgtcgc
      181 ggcgtggctc gccgtcgccg tgtttttcag cgccgagaac atcctcgtcg gcacggcgcg
      241 gcaccggccg ttcacctggc agtgggacgt ctatcacgag ttcgtctact ggctcgtgtg
      301 ggccgcgttc acgccgatca tcctcggcgc cgggcgccgc tggccactgg gcccgggcgc
      361 gctgccccgg gtcatcgccc cgcacctggg cgtgatggcg ctgctcgccc cggcgcagat
      421 caccaccacc tacacggtgc actggctgct gctgagcgcc gtcggtcgtc cgccagccgc
      481 cacgctcggc gcgtggctcg ggggcctcgg gggtgggatc gtctggggca cgctcacggc
      541 gttcctctat tactggctga tcctgggcgt gcacgcggcc ttcgtctacc agcggatgta
      601 tcaggcgcag cgggtcgcca cggccgagct cgagggccgg ctggcccagg cgcagctcga
      661 gacgttgcgg ctgcagctgc acccgcactt tctgttcaac acgctcaacg ccatcgcggc
      721 gttcgtcgga tcggagccgg accgggcacg gcgcatgatc gcccggttgg gtgagctgct
      781 gcgccgcacc ctggacagcg gcacggcggc cgaagtaccc ctggcgcgcg agctcgagct
      841 gctcgcgccc tacctcgaga ttcagcgcat ccgcctcggc gagcggctgc acgtggacgt
      901 ggacatcccc gaggcggcgc gcacggcgct cgtcccaacg ctgctgctgc agcccctggt
      961 ggagaacgcg gtggagcacg gcgtgagccg ccagcccggg agcgctttgg tgcgccttca
     1021 cgcggtacgt gccggagcgc ggttgcggct cgaaatcacc gacgacggac ccgggcctcc
     1081 cgccgcgggt ggcgacgacg gcatcggact cgccaatacg cgcgagcggc tcgtacggct
     1141 gtacggcgcc gcgcaccgtt tcgagctgag cccggccggc gcggtcggga ccacggtcgc
     1201 gatcgagatc ccgttgcgca cggcggaggg cgcctagcgt gcgcgtcgtg atcgtcgacg
     1261 acgagccgcc gggccggctc gccctgcgcg agcagctcgc ggccatgccg gacgccgaga
     1321 tcgtcgccga atgcgagaac ggcgcagcgg cgatcgaggc catcgtgcgt catgcgcccg
     1381 atctcctgct gctcgacgtc cagatgcccg ggctgggcgg cttcgacgtg ctcgagcgcg
     1441 tgggcgcgga cgccgtcccg gcggtcgtgt tcgtcacggc ctacgatcgt tacgccgtac
     1501 gggcgttcga ggcccatgcc gtggactacc tgctcaagcc gatcgatccc gaccgcttcc
     1561 gcgaggcgta ccagcgagcc gcccgccgcg tggagcgcga cggcggggag cacgcgtccc
     1621 gcctcgagaa gttggtggcg ggagacgcgc ccgcgccact cgaccgcttc gtcgtgaagg
     1681 acaaggggcg gctcgtgttc gtgggagtgg agacggtcga ctggatcgag gcggccggaa
     1741 actacgtcac gctgcacgcc ggccccgccg tgtacgggct acggcgtacc ctcgcgagtc
     1801 tcgcgatgcg gctgggcgag cggttcgtgc gcattcgccg ctcggcgctc gtcaatgtgc
     1861 acgccatccg agcgctcgag cagtacggaa aaggcagtta cgtcgtctat ctacgcgacg
     1921 gcaccaagct catttccagc cgctacgccg gccgggagct gcgggcgttg ctcgacacgt
     1981 agccggcgct caggaccccc ggcgcgagcg gcccgccacg acgagcacca gcccgaggcc
     2041 ggcgcccacc cacccgaccc atggagggat cgtgcggcgt tcttcgagcg aggcctggaa
     2101 gtctcccacg cggaggtcgc tccggctgga gcggtaactg accccgcgca gggccacgaa
     2161 caggcccgcg gccaggagca gcaatccggc aatcgaaaca gggcgcatgc ggacgttccc
     2221 gtgtcgtggg gggggcaacg ccgtgcctcg accgccgaag cacgacctcc tacgctacct
     2281 cacgtcgcgc gcgcagaaca gcctccggca tcaaccgcgc gatgggcgtc tgcgaacggc
     2341 ctcctggatc gccccccacg ttcgctccat gtccgcgtgg tcggtccgcc agttcgagat
     2401 cgagacgcgg gccgccggca ctccgcgcag gatggtcggt gtcaggaacg tcacgccccc
     2461 acccttcagc cgttcgagaa actcggtcac ttcctcggcc gatgcacgat cacccccgtc
     2521 acgggcgacc gtgaagcaaa ttccgttgag cttcactggc gcgagcagcc ggaacccggg
     2581 atgctcctcg atccgcgccc ccaaccacgc ggcgtgagcg cagcagcgct cgacgatttc
     2641 ggcgtatccc gcgcgaccgt aggctgcgag cgtcatccac acgggcagcg cgcgcagccg
     2701 ctggctgttc tccggcgtga ggtgcacgag cgtgtccggg cgcacctcag ccggcaggta
     2761 cgccccggag ctttggaacg tctcaccctg cagaccgagg tggcgcgtga gaatgacggc
     2821 attgtcgtac ggcacgttca gccacttgtg accgtcgacg ctcaccgaat ctgcggcctc
     2881 cagcccgtcg agcagcgggc ggtagcgcgg actcgccgcg gcgatcgcgc cgaatgcacc
     2941 gtccacgtgc agccagaacg aatggcgcga tttcaacgcg tctatcgccc ggaggtcatc
     3001 gaagtcacac gtgtttaccg tgccggcatt ggcgacgacg atgacgggat ccacaccctc
     3061 tcgctccgcc gcctgcaggc tcgcggcgag tgcttcgaca tcgacggcct cacgcgacgg
     3121 aagcgtcgcc acgccgtgca gcgcggtgcg accgaggccg atgacggagg cagccttcga
     3181 gatgctggag tgggctgtgc cggagaggat ccggaccggc ccgagggcat gcgccccctc
     3241 gaccgatgcg tcgacgccgc ggctgcgtcc cacccactgg cgcgccgtcg ccagcgcgac
     3301 gatggccgac gtcgtcgcac cggtgacgaa cgcgccctgg aacccatcgg ggagtccgac
     3361 gaggtcgcgc accatggcga gcgcatcgag ggcaatgtgc cgcgcggatg actcgccggc
     3421 atccgacccg ttctggtcgt acaccgacgt gagccagtcg ccgacgaggg cggcgggagt
     3481 ggagcctccc gtgacgaacg cgaagtagcg tggtcccgcg ctcgcactca gccacggcgc
     3541 ataccgttgc cggaacgcct cgagcgcccc cgccgtcccg aaaccgtggg tgggcaggcc
     3601 aagcggggca atcgcgggga ccacgtgcgc gaccggccgc tcgtcgagcg acccgaggaa
     3661 tcgcacagcg tcgtccttcg ccccgccgag gagagcggca agttgctgcc gatctgagga
     3721 cagtactgaa taggtcatgg tgctgccgtc tccctgcggc gtgaggagcg cccacgctcg
     3781 tcgttgggca atccactctg gagggcgccg gtcgcccgcc gatggccagg aggttaaccg
     3841 ggattgaccc ccatcgcgtc ccggcgtacg ttgtaccgaa cggtacattc ccccaggatc
     3901 catgcccccc gccctgttgt cccgcgcaga agtgattgag cgattgatgc tcgtgatccg
     3961 gcgcttcgga tacgacggcg cctcgctcgc cgagctgtcc aaggcgaccg gtctcggcaa
     4021 gtcgagcctg taccactact tccctgccgg gaaggacgac atggttcggg cggtgctgga
     4081 gcatttcgag agtcagctgc aggagctcgt gttcactccg ctgcgtacgc cgggacccgc
     4141 ccgtcgtcgg gtggagggaa tggtcaggac cctggacgcc ttctaccgcg gcggccgtga
     4201 agcgtgcgtg cttggcaatc tcgtgctcgg caccagtcgt actcggttcc gcagtcggct
     4261 gcgggccatc ttcgaccggt ggatcgacgc gctcgcggtg gccctggcgg acagcgggct
     4321 ccccaagtcg gccgctcgcg cgcgggccga ggacgcggtc atgcgcatcg aaggcgcgct
     4381 gatcctcgcc ggcggcatgg acg
//
LOCUS       DBNGZW010000584         2212 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_533648, whole genome shotgun sequence.
ACCESSION   DBNGZW010000584 DBNGZW010000000
VERSION     DBNGZW010000584.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_533648"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1979
                     /locus_tag="AC16JC_13810"
     CDS             114..1979
                     /locus_tag="AC16JC_13810"
                     /inference="COORDINATES: protein motif:HMM:NF019352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR5165820.1"
                     /translation="MAKHAVWCLIGVASITGPGRPTAPATPLIAFNDNRVSAGTLTHG
                     VLTVSLVAQAGEWKPYGADGGGIALFAFGEEGKPLQDPGPLLRVPQGTRIHARVKNLT
                     GKTLVVHGLAARRVAVMDSLVVAAGATGEASFVTDAAGTFYYWGNTGGEGFEDRTGEN
                     ARLNGALIVDPPGLARRPDRVFVIERWMPDTDFWHDVFTVNGRPWPYSERLSYAMGDS
                     IRWRVINASNDVHPLHLHGFYYRVDARGDFAQDTAYWPAQRRMDVTEPIWDGTTMDMV
                     WHADRPGNWIFHCHLNYHVPPNAALAPDTEPTPDRIRHMMSGYPEEHVMDHARYGMGG
                     LVMGITIRPPASWHAYDGARRTLRLLVQSDSAPGDSTRRFGYVLDDGNRAAATAALRA
                     PGPPVVLHRGEPTRIWVVNQTPVMTQVHWHGLEIESFYDGVTGFSGAGTQVEPATMPG
                     DSFEVLVTPPRAGSFMYHTHINDIYQQAHGLYGPLIVLDSGETWNPDRDRVFMVGDNT
                     QYDPVLNGGRLEPLTLSAGAPYRFRLMNITAGSPGLEFWLMHAGALVQWRPIAKDGHA
                     LPSWQTARRPARQPVTIGETYDFRVQAPDTGSVTLEVRAHSGRLIASQAIRFGRP"
BASE COUNT          359 a          764 c          753 g          336 t
ORIGIN      
        1 agtgagatgc gaaaggttgg cctgggtcga gcgcccccgc gtcgaacgcg tccgcgcccc
       61 caccccggcg tcgcgcaaac ttgtgcagag attccccaag taagagaggg caattggcca
      121 agcatgccgt ctggtgcctg atcggtgttg cgtcgatcac gggtccgggc cgtccaacag
      181 ctccagctac tccgctcatc gcgttcaacg acaaccgcgt ctcggccggc acgctgacgc
      241 acggcgtgct caccgtgtcg ctcgtcgccc aggcggggga atggaaaccc tacggcgccg
      301 acggcggcgg catcgcgctc ttcgcattcg gagaagaagg aaagccgctc caggatcccg
      361 gtccgctgct ccgggtcccg caggggacgc ggattcacgc gcgggtcaag aacctcacgg
      421 ggaagacgct ggtcgtgcac ggcctcgccg cgcgccgggt ggcggtcatg gattctctgg
      481 tggtggcggc cggcgccacg ggggaagcga gcttcgtgac cgacgcagcc ggcaccttct
      541 actattgggg caacacgggc ggggaaggct tcgaggatcg caccggggag aatgccaggc
      601 tgaacggcgc gctgatcgtg gacccgccgg gtctcgcgcg ccgtcccgac cgggtgttcg
      661 tgatcgaacg ctggatgccg gatacggatt tctggcacga cgtgttcacg gtcaacggac
      721 gcccctggcc gtacagcgag cggctcagct acgccatggg cgactcgatc cgctggcgcg
      781 tcatcaatgc gtccaacgac gtccacccgc tgcatctgca tgggttctat taccgggtgg
      841 acgcgcgcgg tgatttcgcc caggacaccg cgtactggcc ggcgcagcga cgcatggacg
      901 tcacggaacc gatttgggac ggaaccacga tggacatggt ctggcacgcc gatcgtcccg
      961 gcaactggat cttccattgt cacctgaact accacgtgcc gcccaacgcg gcgctggctc
     1021 ccgacaccga gcccacgccc gaccggatcc gccacatgat gagcggctat ccggaggagc
     1081 acgtcatgga ccacgcccgt tacggcatgg gcgggctggt catggggatc accatccggc
     1141 cgccggcgag ctggcatgcg tacgacggcg cgcgccgcac gctccgtctc ctcgtccaat
     1201 ccgactcggc accgggagac agcacccggc gcttcggata cgtgctcgac gacggcaacc
     1261 gagccgccgc gaccgccgcg ctgcgcgcgc cggggccgcc ggtcgtgctg catcgcggcg
     1321 agccgacccg catctgggtc gtcaatcaga cgccggtgat gacgcaggtg cactggcacg
     1381 ggttggagat cgagagcttc tacgacggcg tcacggggtt cagcggcgcg ggcacccagg
     1441 tcgagccggc gacgatgccg ggggattcct tcgaggtcct cgtgacgccg ccccgcgcgg
     1501 gcagcttcat gtatcacacc catatcaacg acatctacca gcaggcgcac ggcctgtacg
     1561 ggccgctgat cgtgctcgat tcgggcgaaa cgtggaatcc cgatcgcgat cgcgtcttca
     1621 tggtcggcga caatacgcag tacgacccgg tgctcaacgg cgggcgtctc gagcccctca
     1681 cgctctcggc cggcgcgccg tatcgcttcc ggctgatgaa catcaccgcc ggcagtccgg
     1741 gcctcgagtt ctggctgatg catgccggag cactcgtgca gtggcggccg atcgccaagg
     1801 acgggcacgc gctgccgtcg tggcagaccg cgcgacgccc cgcccgtcag ccggtgacca
     1861 tcggcgagac gtacgacttc cgggtgcagg cgcccgacac cgggtccgtc acgctggagg
     1921 tccgggccca cagcggccgg ctgatcgcga gccaggcgat ccggttcggg cgaccctaga
     1981 cggggggccc ggtcgtcgtc ctcgaatcga ggcccgatcg ctctgggcaa tcacctgtga
     2041 ggagcggcac gtggaggatg ctctcatcgc gaatgccggt cgggtcatga gcggccgggc
     2101 caccgcatcc gccaacacca tcgcacgcgg gcgactcctg gtcggcagcg gactgccgct
     2161 cgcgctcgcc gtcgtgtcgt tcgccgcctg caaggacgac atgaccgcga cc
//
LOCUS       DBNGZW010000585        11583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1484557, whole genome shotgun sequence.
ACCESSION   DBNGZW010000585 DBNGZW010000000
VERSION     DBNGZW010000585.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 11583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11583
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1484557"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="AC16JC_13815"
     CDS             complement(<1..597)
                     /locus_tag="AC16JC_13815"
                     /inference="COORDINATES: protein motif:HMM:NF025010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HIR5165821.1"
                     /translation="MRENTKWVFYILAVAFIGWLVFDVGMGVTGRNYGGGDVVLRIDG
                     QAIHLQQWQAALQGALEQARQRGGSNLTREDEKAVEDQLVDQLVRDVLLQHESRRLGI
                     TVTDAEVRDAAMTSPPPEAYSVPDFQTNGQFDPNKWRRFLQSGANPEFLVQLEARYRQ
                     EIPQAKLFQYITAGLYVPDAKLWRIYRDQHDSVTVALLA"
     gene            757..2553
                     /locus_tag="AC16JC_13820"
     CDS             757..2553
                     /locus_tag="AC16JC_13820"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5165822.1"
                     /translation="MAYTSEIEKLEARYRENPKGRNFAPLADAYRKAGLIDNAIELCQ
                     TGLRLHPDYVSGHIVHGRCLVDKKDDTGAEAVFRKVLDLDPENILALKVLAEISERNE
                     RFGAASEWLNRLLLADPMNGEAADGLSRVRGKAAAQPPPPEPAPVEVTLPELALEPEA
                     AAVEEPVAAPLEPSDEVQAPAPLPDTALVVEHEELELKPADAAAQRRLSIEYMEGPFD
                     AGISEAPTAAMEKPEFELERTSEPPAEAGIQPFDGVDFDQVADVGPVEGIVLEEEPAV
                     APDPGVQVEGLARTQYEGSGVFRLDNEPPPAAEPPEERPTSSVDDLPLIMPDEVDSAP
                     APWPEAARPSRETEPLEAPPIPVAASAPLPLRLPDDDGAADTAALSRAEPVVTETMAE
                     LYLRQGHREEALEVYQALSTQRPDDARLRRKVAELSAPPSRGRMAGSGQTAGAFLKGI
                     LSARPGAPPPLLPPESPSTDQASSPASDALGSPGNGPVDRVGLEAPAPGTPTRPASDS
                     ISLDSVFGEEGGRGSAPVLPEPAPEQPAKLPVASGGFSFDDFFGATTVPAAGGAGKAP
                     GSRPSRPSGRNRSPEQEEDLDQFQAWLKSLKA"
     gene            2553..2996
                     /gene="aroQ"
                     /locus_tag="AC16JC_13825"
     CDS             2553..2996
                     /gene="aroQ"
                     /locus_tag="AC16JC_13825"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006911540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HIR5165823.1"
                     /translation="MRIAVLNGPNLNLLGVREPEVYGRATLEEIEAATRAQAVALGVE
                     CVWTQTNHEGELVEAVQQLRGTVDGALVNAAAFTHTSLAVRDALLAAQVPFVEVHLSN
                     IFAREPERRRSLLADLAVGIVSGFGADSYRLALDGLVRHLAAHGG"
     gene            2989..4083
                     /locus_tag="AC16JC_13830"
     CDS             2989..4083
                     /locus_tag="AC16JC_13830"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR5165824.1"
                     /translation="MDEEARSARQEALVRLLEAEGLDALLVTSLPNIRYLTGFSGSAA
                     LVLAAASGVLLVTDFRYEAQAEDEIGGAARVVVERTSAWDRLFREIASAGQPRTIGFE
                     AHALSAKDAERFATAGKAWRWRAAPELVERLRVRKSPAEIAAVRAAASLAGDALRATL
                     GAVRCGQTEREIAGLLEGHLRRLGSEGHPFPTIVASGARTVWPHARTSARPVARGEWL
                     LLDFGAQVDGYCADITRTVVVGARATDAQRVLYDLVHAAQRTARERVRAGMSAREADA
                     LARDLIDARGFGDAFGHSLGHGLGLEVHEAPRLARTNAEPLPVDAVVTIEPGIYLPGQ
                     GGVRLEDDVHLAAAGPVLLSDGATDLVELV"
     gene            4157..4648
                     /gene="accB"
                     /locus_tag="AC16JC_13835"
     CDS             4157..4648
                     /gene="accB"
                     /locus_tag="AC16JC_13835"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HIR5165825.1"
                     /translation="MNLDLIGQLVKILRDTPELGAIEVRRGLLGLWSSVRVSKAGLTA
                     GNPGSVVVAAPAAVAPAAQSPPAGAAPAVPSPAAAAPSALLEIKSPMVGTFYKAPEPG
                     AEPYVKVGTRVSPGQVVCIIEAMKIMNEIESEVQGTIREVCVENAQPVEFGQVLFRVD
                     PHG"
     gene            4641..5807
                     /locus_tag="AC16JC_13840"
     CDS             4641..5807
                     /locus_tag="AC16JC_13840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR5165826.1"
                     /translation="MAEAPQGNPPPFNFKAAITQMVQRNGSDLLLKVGRPATVRVNGD
                     LVSLETQPLKPEELKSLAEQIMTPRQVKEFAEHKEADFAIGVPGVGRFRTNVYQQRGT
                     VAFAFRAIPYEVKTIQELNLPPVLEEIALRPRGLVLVTGVTGSGKSTCLAAMINHVNA
                     SRRVNIITIEDPIEFLHRDNLANISQREVGNDTLSFGSALRHVLRQDPDVILIGEIRD
                     METLDTALKAADTGHLVFSTLHTTDATQTINRVISFFPPHQHDEVRHLLSTALQAVVS
                     LRLVPRKDAKGRVPAAEVLINTAAVAENVRDLTKSLNIPDLIAEGSIQYGMQSFDQSL
                     FQWYQHGVISYESAIFYATNPSEFALKVSGVDASGSKLSGPGAGAGVESIGLTS"
     gene            5807..7165
                     /gene="accC"
                     /locus_tag="AC16JC_13845"
     CDS             5807..7165
                     /gene="accC"
                     /locus_tag="AC16JC_13845"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007786381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HIR5165827.1"
                     /translation="MFQKVLIANRGEIALRVIRACKELGIATVAVYSEADRECLHVRF
                     ADDDVCIGQPPSRDSYLNISRIIAAAEITGADAIHPGYGFLAENAEFAEIVRTSGIAF
                     IGPTPDQIRQMGDKAAARELAQQLKVPTVPGSPGPVESLEEALAVATGIGFPVIIKAA
                     AGGGGKGMRVAVDADAFTQAYNLARQEALAAFGSDQVYIEKYLARPRHIEIQIMGDQH
                     GRIMHVCERDCSVQRRHQKLIEEAPSPALDQALRDRIGGAAVRLAEAIGYVGAGTIEF
                     LLDEDGSFYFMEMNTRIQVEHPVTEMCTNFDLVKEQIRVAAGEPLSLMLHGNRLRGHA
                     IECRVNAEDPARNFQPSPGLISTFHPPGGPGVRVDTHIYAGYTVPPYYDSLLAKVIVH
                     GNDRAEAIARMRQALDSFIIEGVRTTIPFLGRVMRHPDFVAGQIDTKFLEREPQLLKD
                     PE"
     gene            7162..7881
                     /locus_tag="AC16JC_13850"
     CDS             7162..7881
                     /locus_tag="AC16JC_13850"
                     /inference="COORDINATES: protein motif:HMM:NF015962.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HIR5165828.1"
                     /translation="MKLDVYFTPPGPTAGDLAGRGVVVIDVLRATTTIIAALANGARA
                     VVPTASSEEAVKMTANLERDGFVLAGERRYLPIPGFALGNSPREMTAEQVGAKTVYLA
                     TTNGTPALVAAQGADPVLVGSAVNFGALADRAGALLRERGDLAIICAGRERQFALEDA
                     YVAGRLVKMAKKGLRKLALNDGAEVSLALTQRYKNFLDAFTASEAGRSLESLQLGDDV
                     RFSAKMDRYALVPAYADRRIT"
     gene            7878..10130
                     /locus_tag="AC16JC_13855"
     CDS             7878..10130
                     /locus_tag="AC16JC_13855"
                     /inference="COORDINATES: protein motif:HMM:NF013727.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE family DNA translocase"
                     /protein_id="HIR5165829.1"
                     /translation="MNPKARAELGGVGALVVGLFLGLTLLPWHLTGGLGERLGNFCWQ
                     TLGVGAVLLPVLGVGWALAAFGRLGSLSTPRAAALVGGLVVLVPFAIGVVGRVDLAFP
                     PDYRGWSPSQQLVGVLPGWLASVAVRTVGTAGGVLVGVFALSALGILTIGWHPLAILR
                     AGGETAGEGVEEGGRSRRPPKAAKPVAGDEAEPVGAGIAVMPARERPERPEKAEKQRR
                     AKQGPASRAPAAPLPEGVLIPPPELLSAPTSEELDPGTEEIEAMGQRLVQTLETFRVG
                     GTIADRTVGPVVTRYELEPGPGVKVGRIASLADDLALAMKARSLRIVAPIPGKARVGI
                     EVPNPRARMVTIRELVDNARFRTPGRILPVVLGRDLEGQEVYDDLAKMPHLLIAGATG
                     SGKSVCINAIITSLIYSHPPDRLQMLMVDPKMVELSMYGDLPHLRLPVVTSHHKAATV
                     LKWAQGEMERRYRLLHANGARNMADFNRKAETGRPLKGPRETLATQAGVTRELPFEEG
                     AYKDGILPYIVLVVDELADLMLTVQGEIETPLASLAQKARAIGIHLILATQRPSVNVI
                     TGLIKANFPCRIAFRVSAKVDSRTILDQNGAETLLGNGDMLFLPPGKSEPVRVQGAFI
                     STDETERIMEWYRNRKAADGTASVRAVMEQQIAAIEAAEAAERGGGEGGGEDMGDRDP
                     LFRQAAEVCIQNQLGSTSLLQRRMSIGYGRAARIIDQLEMAGILGPANGSKPRDVLVG
                     LEELDEICGT"
     gene            10279..10671
                     /locus_tag="AC16JC_13860"
     CDS             10279..10671
                     /locus_tag="AC16JC_13860"
                     /inference="COORDINATES: protein motif:HMM:NF014119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR5165830.1"
                     /translation="MHRGLRSDPSEWTDERHKQGLAAEEQAIRFLASRGWLVLAHRWR
                     VGRTEIDIVARRGVTVAFIEVKSRRGEAFGSPFEAVTGAKQRELVRAARAWIDRFGVP
                     ADIYRFDCIAVRQGVMEHLEDAFRPGWR"
     gene            10732..>11583
                     /gene="recA"
                     /locus_tag="AC16JC_13865"
     CDS             10732..>11583
                     /gene="recA"
                     /locus_tag="AC16JC_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR5165831.1"
                     /translation="MTRILMAAEDRETAKLTDERRKALNLAIAQIEKTCGKGSIMRMG
                     SESPRVRVDAISTGAINLDAAIGVGGIPRGRVTEIFGPESSGKTTLALSVVANAQRTG
                     GAAAYIDAEHALDIEYAKKLGVDIDNLLVSQPDTGEQGLEITEILVRSGAVDIVVVDS
                     VAALVPKAEIEGDMGDSHVGLQARLMSQALRKLAGAINRSRTSVVFINQLCEKIGIMF
                     GNPETTTGGRALKFYASVRLDMRRIAPVKEREVVIGNHVRVKVVKNKVAPPFRQAEFD
                     IMFDEGIS"
BASE COUNT         1874 a         3797 c         4150 g         1762 t
ORIGIN      
        1 cgcgagcagc gcgacagtca cggaatcgtg ctggtcacgg taaatgcgcc acagcttcgc
       61 atccgggacg tacagaccag cggtgatgta ctggaacagc ttggcctggg gaatctcctg
      121 ccggtagcgc gcttcgagct gcaccaggaa ctcggggttg gcgccgctct ggaggaaacg
      181 gcgccacttg ttcgggtcga actgcccgtt cgtctggaag tcggggacgg agtaggcctc
      241 gggaggcgga gacgtcatcg ccgcatcgcg cacttcggcg tcggtcacgg tgatgccgag
      301 tcggcggctc tcgtgctgca gcagcacgtc gcgcaccagc tggtcgacga gctgatcctc
      361 gacggctttc tcgtcctcgc gggtcaggtt ggatccgccg cgctgccgcg cctgctcgag
      421 ggctccctgg agcgccgctt gccactgctg cagatggatg gcctgcccat cgatgcgcag
      481 gaccacgtcg ccgccgccgt aattcctgcc ggtcaccccc atccccacgt cgaacaccag
      541 ccagccgatg aaggcgacgg cgaggatgta gaagacccat ttcgtattct cgcgcatcgc
      601 gcgcatcata ccgcagggta ctccgtgtca ggggggcgaa gggggataag tctaagagcc
      661 acaacgaaca aactcaagcg agcagacctc gcgtggtaat ttgacagcca gggcggccga
      721 gcactatctt agaccaatcc ccacaaagac ttaggcgtgg cgtacaccag cgagatcgaa
      781 aagctcgaag cgcgctaccg ggagaatccc aaggggcgga acttcgcgcc gctggcagac
      841 gcgtatcgca aagcggggct tatagataac gcgatcgaac tgtgtcagac cggtctgcgg
      901 ctgcatcccg actacgtcag tggccacatt gttcacggac gctgcctcgt tgacaagaaa
      961 gatgacaccg gcgccgaagc ggtgttccgc aaggtgctcg atctcgatcc cgaaaacatc
     1021 ctcgccctca aggtgctcgc ggaaatctcg gagcggaacg agcgcttcgg tgcggcgtcc
     1081 gagtggctga accggttgtt gctggcggat ccgatgaacg gcgaggccgc cgatgggttg
     1141 tctcgggttc gtggcaaggc cgccgcgcag ccgccgcctc cagaaccggc gccggttgaa
     1201 gtgacgctgc cggagctggc gctggaaccc gaggcggcgg ccgtcgagga gccggtcgcc
     1261 gcgccgctcg agccgtccga cgaggtgcag gctcctgcgc ccctgccgga cactgcactc
     1321 gtcgtggaac acgaggagtt ggagttgaag cctgccgatg ccgccgcaca gcggcgcctg
     1381 tccatcgagt acatggaggg gccgttcgac gccgggatct cggaagcccc cacggccgcg
     1441 atggagaagc ccgagttcga gctggagcgc acgtccgagc cgccggcgga agccggcatc
     1501 cagccattcg acggcgtcga tttcgatcag gtggcggatg tcggtccggt cgaaggcatc
     1561 gtgctcgagg aagaaccggc tgtggcgccg gaccccgggg tccaggtcga gggactggcc
     1621 cgcacgcagt acgaaggcag cggggtgttt cgcctggaca acgagccgcc accggcggcg
     1681 gagccgcccg aggagcgacc gacgagctcc gtggacgatc tgccgctcat catgcccgat
     1741 gaggtcgact ccgctcccgc gccctggccc gaagcggcgc gaccgtcccg cgagaccgag
     1801 ccgctggaag cgccaccgat cccggtggcc gcctcggcgc cgcttcccct gcgcctcccc
     1861 gacgatgatg gcgccgctga caccgcggca ttgtcgcgag ccgagccggt ggtcacggaa
     1921 acgatggccg agttgtatct gcggcagggc catcgggagg aggcgttgga ggtctatcag
     1981 gcgctgtcga cgcagcggcc ggacgacgcc aggttgcggc gcaaggtggc cgagctctcc
     2041 gctccgccgt cccgcggccg catggcaggc agcgggcaga cggccggggc gttcctgaag
     2101 ggaatcctca gcgcgcgccc cggagctccg ccaccgctac tcccccccga gtcgccgtcg
     2161 accgatcagg catcgagccc cgcgtccgac gcgctgggca gcccgggcaa cgggccggtg
     2221 gaccgggtcg ggctggaggc accggcgccc ggcacgccga cgcgtccggc gagcgactcg
     2281 atctcgctcg actccgtgtt cggtgaagaa ggcggacgcg gctcggcgcc cgtgctgccc
     2341 gaacccgctc ccgagcagcc cgcgaagctg ccggtcgcga gtggcggctt ttccttcgat
     2401 gatttcttcg gcgcgacgac ggtcccggcg gccggcggcg ccgggaaggc gccgggatcc
     2461 cggccgtcgc gaccttctgg gcgcaaccgc tctccggaac aggaagagga cctggatcag
     2521 ttccaggcct ggctcaagag cctcaaggcc tagtgcgcat cgccgttctg aacggcccca
     2581 atctcaacct cctgggcgtc cgtgaacccg aggtctacgg gcgcgcgacg ctcgaggaaa
     2641 tcgaggcggc cacgcgcgct caggccgtcg cgctcggcgt cgagtgcgtc tggacgcaga
     2701 cgaaccacga aggggagctg gtggaggccg tacagcagtt gcggggcacg gtggatggcg
     2761 cgctggtcaa cgcggcggcg ttcacgcaca ccagtctggc cgtgcgggac gcgctgctgg
     2821 cggcgcaggt gccgttcgtc gaagtccatc tctcgaacat cttcgcgcgg gagccggagc
     2881 gccgtcgctc attgctcgcc gatctcgccg tggggatcgt gagcggcttc ggcgcagaca
     2941 gttaccggct cgccctcgac ggcctggtgc gccatctcgc cgcacacggt ggatgaggaa
     3001 gcgcgctcgg cgcggcagga ggcgctcgtc cggctgctcg aggcggaagg gctggacgct
     3061 ctgctggtaa cttcgttgcc gaacatccgc tacctgacgg gattcagcgg atcggcagcc
     3121 ctggtgctgg cggccgcgag cggtgtgctc ctggtgacgg acttccgcta cgaagcccag
     3181 gcggaggacg aaatcggcgg cgcggcccgc gtggtggtcg agcgtaccag cgcctgggac
     3241 cgcctgttcc gcgagattgc gtctgctggc cagccgagga cgatcggttt cgaggcgcac
     3301 gccctctcgg cgaaggacgc cgagcggttt gcgaccgcgg ggaaggcctg gcgctggcgc
     3361 gcggcgccgg agctcgtgga acggctgcgc gtacggaaga gtccggcgga gattgccgcc
     3421 gtgcgcgcgg ccgcgtccct ggccggtgac gcgctgcgtg ccacgctcgg cgcggtgcgg
     3481 tgcggtcaga cggagcgcga gatcgcgggt ctgctcgagg gccacctgcg ccgtctgggc
     3541 agcgaggggc atccgttccc gacgatcgtc gcctccgggg cgcgcacggt gtggccccac
     3601 gcgcgcacca gcgcgcggcc ggtggcgcgt ggggagtggc tgttgctcga cttcggcgcg
     3661 caggtggacg gctactgcgc ggacatcacg cggacggtcg tggtgggcgc gcgcgccacg
     3721 gacgcgcaac gggtgctgta cgatctggtg cacgccgcgc agcggacagc gcgggagcgg
     3781 gtgcgggcgg gcatgagcgc gcgcgaggct gacgctctgg cgcgcgacct gatcgacgcg
     3841 cgcggcttcg gggatgcgtt cgggcattcg ctgggacacg gcctgggtct cgaggtgcac
     3901 gaagcccccc ggctcgcccg caccaatgcg gaaccgctgc cggtcgatgc cgtcgtgacg
     3961 atcgagccgg ggatctacct gccggggcag ggcggcgtgc gtctggaaga cgacgtccac
     4021 ctggcggccg cggggccggt gctcctgtcc gacggggcga ccgacctggt ggagctggtg
     4081 tgacgtcgga gccgcacatt gacggattgc ttctccgggt ggtctatttg gacctcgtgc
     4141 cacgacctgt gacgacatga acctggatct catcgggcag ctggtcaaaa tcctccgcga
     4201 tactccagaa ctgggggcga tcgaggtgcg ccgcggactg ctcgggctct ggtcgtccgt
     4261 gcgggtctcg aaggccggcc tgacggcggg gaaccccgga tctgtcgtgg tggcggcccc
     4321 ggcggccgtc gcgccggccg ctcagtcgcc gcccgccggc gcggcgccgg ccgtgccgtc
     4381 tccggcggcg gctgcgccga gtgcgctgct cgagatcaag tcgccgatgg tcggtacgtt
     4441 ctacaaggcg ccggagccgg gggcggagcc ctacgtgaag gtggggacgc gcgtctcacc
     4501 ggggcaggtc gtgtgcatca tcgaggcgat gaagatcatg aacgagatcg agtccgaggt
     4561 gcagggtacg atccgcgagg tctgcgtcga gaacgcgcag cccgtcgaat tcggccaagt
     4621 cctcttccgg gtggatcccc atggctgagg caccgcaggg aaatccgccg ccgttcaact
     4681 tcaaggcggc gatcacgcag atggtccaac gcaacggctc ggatctgctg ctcaaggtcg
     4741 gccggccggc gacggtgcgg gtcaacgggg atctggtgtc gctggagacc cagcctctga
     4801 aacccgagga gctcaagtcc ctcgccgagc agatcatgac gccacgtcag gtgaaagagt
     4861 tcgccgagca caaggaggcg gatttcgcca tcggcgtgcc gggcgtcggc cgtttccgca
     4921 ccaacgtgta ccagcagcgc ggcacggtgg cgttcgcgtt ccgggcgatc ccctacgagg
     4981 tgaagacgat ccaggagctc aacctgccgc cggtgctgga ggagatcgcg ctgcggccgc
     5041 gcgggctggt gctggtgacc ggcgtgaccg gctcgggaaa atccacctgt ctcgcggcga
     5101 tgatcaacca cgtcaatgcg agccggcggg tcaacatcat cacgatcgag gacccgatcg
     5161 agttcctgca ccgggacaat ctggccaaca tctcgcagcg cgaggtcggg aacgacacgc
     5221 tgtcgttcgg ctccgccctg cggcacgtcc tgcggcagga ccccgacgtg atcctgatcg
     5281 gggaaatccg cgatatggaa acgctcgaca cggcactcaa ggcggcggac accggccacc
     5341 tggtgttctc cacgttgcac acgaccgacg ccacgcagac gatcaaccgg gtgatctcgt
     5401 tctttccgcc gcaccaacac gacgaggtgc gccacctgct ctccacggcg ttgcaggcgg
     5461 tggtctcgct gcgactcgtg ccgcgcaagg acgccaaggg gcgggtgccg gcggcggagg
     5521 tgctgatcaa caccgcggcc gtggccgaaa atgtccgcga cctcaccaag tcgctcaaca
     5581 tcccggacct gatcgccgag ggctcgattc agtacgggat gcagagcttc gatcagtcgc
     5641 tgttccagtg gtaccagcac ggcgtgatca gctatgagag cgccatcttt tacgcgacca
     5701 atccgagcga gttcgcgctc aaggtttcgg gcgtggacgc gtccggcagc aagctcagcg
     5761 gacccggcgc gggcgccgga gtcgagtcga tcggtctcac cagctgatgt tccagaaggt
     5821 gctgatcgcc aaccgcgggg agatcgccct gcgggtgatt cgtgcctgca aggagctggg
     5881 gatcgcgacc gtcgccgtgt attccgaggc ggaccgcgaa tgcctgcacg tgcggttcgc
     5941 cgatgacgat gtctgcatcg gccagccccc ttcccgcgac agctacctca acatctcccg
     6001 catcatcgcg gccgccgaga tcacgggggc ggatgccatt caccccgggt acgggttcct
     6061 ggcggagaac gcggaattcg ccgaaatcgt ccgcacctcg gggatcgcct tcatcggtcc
     6121 gacgccggat cagatccgcc agatggggga caaagcggcc gcgcgcgagc tggcacagca
     6181 gctcaaggtg ccgaccgtgc cggggtcccc ggggccggtg gaaagcctgg aagaggcgct
     6241 ggcggtggcg accgggatcg ggtttccggt gatcatcaag gcggcggcgg gtggtggggg
     6301 gaaggggatg cgcgtggcgg tcgacgccga cgcgttcacc caggcctaca acctcgcccg
     6361 gcaggaggcg ctcgcggcct tcggctccga tcaggtctac atcgagaagt acctcgcccg
     6421 tccccggcac atcgaaatcc agatcatggg cgatcagcac ggtcgaataa tgcacgtgtg
     6481 cgaacgggac tgctcggtgc agcggcgcca ccagaagctg atcgaggagg cgccgagccc
     6541 ggcgctcgac caggcactgc gcgatcgcat cggcggggcg gcggtgcggc tcgccgaagc
     6601 gatcggctac gtgggcgcgg gcaccatcga gttcctgctc gatgaagacg gctcgttcta
     6661 cttcatggag atgaacaccc ggatccaggt cgagcacccc gtgaccgaga tgtgcacgaa
     6721 cttcgacctc gtgaaggagc agatccgggt cgcggcaggc gaaccgctgt cgctcatgct
     6781 gcatggaaac cggctgcgcg gccacgccat cgaatgccgc gtcaacgcgg aagaccctgc
     6841 ccgcaacttc cagccctcac ccgggctcat ctccacgttc catccgccgg gcggccccgg
     6901 cgtgcgggtc gatacgcaca tctacgccgg ctacaccgtg ccaccctact acgactcgct
     6961 gctcgccaag gtcatcgtgc acggcaacga ccgcgccgag gccatcgccc gtatgcgcca
     7021 ggccctcgac tcgtttatca ttgagggcgt gcggacaacg atcccgttcc tgggacgggt
     7081 gatgcgtcat ccggacttcg tcgcggggca gatcgacacc aagttcctcg agcgcgagcc
     7141 gcagctcctc aaagatcccg aatgaaactc gacgtctact tcaccccgcc cggcccgacc
     7201 gcaggagacc tcgccggccg gggggtcgtc gtcatcgatg tgctgcgggc caccaccacc
     7261 atcatcgccg cgctggccaa cggcgcgcgc gccgtcgtcc cgaccgcgtc gtcggaggaa
     7321 gcggtcaaga tgaccgccaa cctcgagcgc gacggcttcg tgctcgcggg tgagcggcgc
     7381 tacctgccga tccccggctt cgcgctcggc aactcgccgc gcgagatgac ggccgagcag
     7441 gtcggcgcca agaccgtgta cctggcgacc accaacggca cgccggcgtt ggtcgccgcc
     7501 cagggtgccg acccggtgct ggtgggctcc gcggtgaact tcggggccct cgccgaccgg
     7561 gcgggggccc tgctgcgcga gcgcggcgac ctggcgatca tctgcgccgg gcgggagaga
     7621 cagttcgccc tcgaagacgc ctacgtggcg gggcgactcg tgaagatggc caagaaggga
     7681 ctgcgcaagt tggccctgaa cgacggcgcc gaggtgtcgc tcgcgctgac ccagcgatac
     7741 aagaacttcc tggatgcctt cacggcctcc gaggcgggac ggtcgctgga gtcgctgcag
     7801 ctgggcgacg acgtccgctt cagtgcgaag atggatcgct acgcgctcgt ccccgcctac
     7861 gcggaccgcc gtatcacatg aaccccaagg ctcgagcgga gctgggcggc gtcggcgcgc
     7921 tcgtcgtcgg gctcttcctc ggcctcaccc tgcttccctg gcacctcacc ggcgggctcg
     7981 gcgagcgact ggggaacttc tgttggcaga ccctgggggt cggggcggtc ctgctaccgg
     8041 tgctgggtgt gggatgggcg ctcgccgcgt tcgggcgcct ggggtccctc tcgacgccgc
     8101 gggcggcggc cctggtcggt ggcctcgtgg tgctggtgcc gttcgccatc ggggtcgtgg
     8161 gccgtgtgga tctcgcgttc ccccctgact accggggatg gagcccatcc caacagctcg
     8221 tcggcgtcct cccggggtgg ctggcgagtg tggcggtgcg caccgtcggg acggcgggcg
     8281 gcgtcctcgt cggggtgttc gcgctctccg cgctcggcat cctcacgatc ggttggcatc
     8341 cgctcgccat tcttcgcgcc ggcggcgaga cggcgggaga gggcgtggag gagggggggc
     8401 gctcccggcg tccgcccaag gcggcgaagc cggtggccgg cgacgaagcg gagccggtgg
     8461 gcgcgggtat cgcggtcatg ccggcgcggg agcggccgga gcggccggag aaggcggaga
     8521 aacagcgccg tgccaagcaa ggccccgcct cccgtgcccc cgccgctcca ctccccgaag
     8581 gcgtgctgat cccgccaccc gagttgctga gcgcgccgac gtccgaagag ttggatcccg
     8641 ggaccgaaga gatcgaggcg atgggtcagc gcctggtgca gactctcgag acgttccgtg
     8701 tgggcggcac gatcgccgac cgcaccgtgg ggcccgtcgt aacgcgctac gagctcgagc
     8761 cggggccggg ggtgaaggtg gggcggatcg cctccctggc cgacgacctc gcactcgcga
     8821 tgaaggctcg cagcctgcgg atcgtggcgc cgatcccggg gaaagcacgt gtcggcatcg
     8881 aggtccccaa tccgcgggcc cggatggtca cgatccgcga gctggtggac aacgcccggt
     8941 ttcgcactcc gggccgcatc ctgccggtgg tgctcggccg cgatctcgag gggcaggagg
     9001 tgtacgacga cctcgccaag atgccgcatt tgctcatcgc cggcgcgacg gggtcgggaa
     9061 agtcggtctg catcaacgcc atcatcacga gcctgattta ctcgcatccc cccgaccggc
     9121 tgcagatgct gatggtggat ccgaagatgg tcgagctctc gatgtacggg gacctgccgc
     9181 acctgcggct cccggtcgtg acgagtcacc acaaagcggc gacggtgctc aaatgggcgc
     9241 agggggagat ggagcgtcgc taccgcctgc tgcacgccaa cggcgcccgc aacatggccg
     9301 atttcaaccg caaggcggag accggccggc cgctgaaggg gccgcgtgaa acgctggcca
     9361 cgcaggcggg cgtgacgcgg gaactgccgt tcgaagaagg tgcgtacaag gacggcatcc
     9421 ttccctacat cgtcctggtg gtggacgagc tggccgacct gatgctcacg gtccagggcg
     9481 agatcgagac gccgctcgcc tccctggcgc agaaggctcg cgccatcggc attcatctga
     9541 ttctcgcgac ccagcgtccc tcggtgaacg tgatcaccgg gctcatcaag gcgaatttcc
     9601 cctgccgcat cgccttccgg gtctcggcca aggtggacag ccgtaccatt ctcgaccaga
     9661 acggcgccga gacgctgctg ggcaacggcg acatgctgtt tctccccccc ggcaagagcg
     9721 agccggtccg cgtgcagggc gcgttcatct ccacggatga gaccgagcgc atcatggagt
     9781 ggtatcggaa ccgcaaagca gcggacggca ccgcgtcggt ccgggcggtc atggagcagc
     9841 aaattgcggc gatcgaagcc gcggaggcgg cggagcgggg cggtggggag ggcggcgggg
     9901 aggacatggg agaccgggat ccgctgttcc ggcaggcggc agaggtgtgc atccagaacc
     9961 agctcggttc cacctcgttg ttgcagcgtc gcatgagcat cgggtacgga cgcgccgccc
    10021 ggatcatcga ccagctggag atggcgggaa tcctcgggcc cgccaacggc tcgaagccac
    10081 gcgacgtgct ggtggggctg gaggagctgg atgagatctg tgggacgtga gctggccatc
    10141 agctatcggc tatcggctat cggccatcgg cctacgaccg acccatagct gatagccgat
    10201 agccgatagc gcattttttg ggcaccggtc gcgcagttcg ggtgacggag gtcggccgcc
    10261 gcgcactagc ttcccctcgt gcatcgcggc ctgcgcagcg acccgagcga gtggacggac
    10321 gagcggcaca agcaagggct cgccgccgag gagcaggcga tccggttcct cgcgagccgg
    10381 ggctggttgg tgctggcgca ccggtggcgc gtcgggcgga ccgagatcga catcgtggcg
    10441 cgccgtgggg tgacggtcgc tttcattgaa gtgaagtcgc ggcgcggtga ggcattcggc
    10501 agtccgttcg aggcggtgac cggcgcgaag cagcgggagc tggtgcgggc ggcccgcgcc
    10561 tggatcgacc gcttcggcgt tcccgcggac atctaccggt tcgattgcat tgcggtgcgg
    10621 cagggcgtaa tggaacacct ggaggatgcg ttccgcccgg ggtggcggta atccaggctt
    10681 cggtatatat tcgggtctcg cgcaccaccc ccacggcgag gctcccaacc gatgacgagg
    10741 atcctgatgg cggccgaaga ccgcgaaacg gcgaaactga ccgacgagcg ccgcaaggcg
    10801 ctgaatctgg ccatcgccca gatcgagaag acctgcggca aggggtcgat catgcgcatg
    10861 ggatcggagt ccccccgggt acgggtggac gcgatctcca ccggcgccat caatctcgac
    10921 gcggcgatcg gggtgggcgg catcccccgc ggccgggtga ccgagatctt cggtccagaa
    10981 tcgagcggca agaccacgct ggcgctgtcg gtggtggcga acgcccagcg gacgggcggc
    11041 gcggccgcct atatcgacgc cgagcacgcg ctcgacatcg agtacgccaa gaagctgggc
    11101 gtggatatcg acaacctgct cgtgtcccag cccgacacgg gagagcaggg actcgagatc
    11161 actgagatcc tggtgcgctc gggagccgtg gacatcgtgg tggtcgattc ggtggccgcg
    11221 ctcgtgccga aggccgagat cgaaggcgat atgggggact cccacgtcgg gctgcaggcg
    11281 cggctgatga gccaggcgct gcgtaagctc gccggggcca tcaaccgcag ccgcacgtcc
    11341 gtggtcttca tcaatcagct gtgcgagaag atcggcatca tgttcggcaa cccggagacc
    11401 accaccggcg gccgggcgct caagttctac gcgtcggtcc gcctggacat gcggcgcatt
    11461 gcgcccgtga aagagcggga ggtggtgatc ggcaatcacg tacgcgtgaa ggtcgtgaag
    11521 aacaaagtgg cgccgccgtt ccgccaggcc gagttcgaca tcatgttcga cgagggcatc
    11581 agt
//
LOCUS       DBNGZW010000586         3043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1349703, whole genome shotgun sequence.
ACCESSION   DBNGZW010000586 DBNGZW010000000
VERSION     DBNGZW010000586.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1349703"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1051)
                     /locus_tag="AC16JC_13870"
     CDS             complement(<1..1051)
                     /locus_tag="AC16JC_13870"
                     /inference="COORDINATES: protein motif:HMM:NF013179.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR5165832.1"
                     /translation="MRLATRFFLSASLLVAATVLGLTIAADRILRDRLELQTAEGLER
                     EARLVARLIPTDSTAWPAIAIQLGTLIGHRVTLIDSTGRVRGDTEFPADALPQLENHA
                     HRPEVMAALATGIGESKRYSVSTDVNRMYLAIRGGPSGLSVVRVSATLAAVDAPINAI
                     QRAIWLAGAVAIALAAVFAFVGSRYYALQLSRLTSAARAIAEERSPAFPDSRVPEIAQ
                     HVRALRAMHDELDTRFAALRREREETAALVEAMTDGVVATDARGVIRTVNAAARRLLR
                     VEPAATLPPLAELFHDQAARALVEDIRAGRDVAERALRLGERHVVAAIHPLPDGGSLL
                     VLRDISDLRRLETVRR"
     gene            complement(1052..1783)
                     /locus_tag="AC16JC_13875"
     CDS             complement(1052..1783)
                     /locus_tag="AC16JC_13875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5165833.1"
                     /translation="MPPDADAAPPQRVLVVDDEPDIAGLVAYHLAKAGYRVTTAGDGT
                     EALTRARAERPDLVVLDLMLPGRSGLDVLAELRRTEETRHMGVILLTARREEQDRIAG
                     LERGADDYLAKPFSPQELVLRVGAVLRRLAAVPVAAGGILSAAGVRLDPAAHRVTVND
                     VEIELTATEFKLLRLLMERSGRVQSRTDLLETVWQAQPDIQTRTVDMHVQRLRTKLGA
                     AGDCVETVRGVGYRFRVPPARDRAR"
     gene            complement(1773..2126)
                     /locus_tag="AC16JC_13880"
     CDS             complement(1773..2126)
                     /locus_tag="AC16JC_13880"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR5165834.1"
                     /translation="MSPADYLTTLAEPTRLRVLNCLAATTLYVSDLVGILDLPQPTVS
                     RHLRVLRTMGLVQNQRCATRVAYHLTLPAGAQGRMVRGVLHALKSDPAFRADAAAARA
                     RAAAPRNPRHAAHAP"
     gene            2178..>3043
                     /locus_tag="AC16JC_13885"
     CDS             2178..>3043
                     /locus_tag="AC16JC_13885"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HIR5165835.1"
                     /translation="MRFLLSIVVAVQQAPLPSPDTAHVVIVATTDVHGRVLGWDYVRD
                     RAARGGLSRAAAILTTLRAQYPGEVILVDAGDLLEGNPFAKYFGRVERRRPDPVVDAL
                     NALQYDAATPGNHDFDFGLDLLASADSDATFRYVSANVVMTVGGGGGSRDSLLFPATV
                     VIQRGPVRVGITGFTTPGVNVWDRRQLHGRVHVRPIAAAAPAALAALERAGVDLKVVL
                     VHSGLGEASSYDTTGVGPENDAAALARVTPRPDVVVVGHTHREIRDSVINGVHFVQPR
                     NWALSLSVVHVWL"
BASE COUNT          421 a         1033 c         1156 g          433 t
ORIGIN      
        1 cgcggcgcac cgtctcgaga cggcgcaggt cggagatatc gcgcaggacg agcaggctgc
       61 cgccgtcggg caaggggtgg atggcggcga cgacatggcg ctcgccgagc cggagcgcgc
      121 gctccgctac gtcccgtccc gcccggatgt cctccacaag ggcgcgggcc gcctggtcgt
      181 gaaacagctc cgccagaggc ggcagggtgg cggccggctc tacgcgcagc aggcggcgtg
      241 cggcggcgtt gaccgtgcgg atcactccac gggcgtcggt ggcgacgacc ccgtcggtca
      301 tcgcctcgac cagggcggca gtctcctcgc gctcgcggcg cagggcggcg aagcgggtgt
      361 cgagctcgtc gtgcatcgcc cggagcgccc gcacatgctg cgcgatctcg gggacgcggc
      421 tgtccggaaa cgccgggctt cgctcttcgg cgatggcccg agccgcgctg gtgagtcggg
      481 acagctgcag cgcatagtac cggctcccca caaaggcgaa gacggccgcg agggcgatcg
      541 ccaccgctcc cgcgagccag atcgcgcgct ggatggcgtt gatgggcgcg tcgacggcgg
      601 cgagcgtcgc cgagacccgg acgacggaaa gccccgacgg ccccccgcga atcgcgaggt
      661 acatgcggtt gacgtcggtc gagacgctgt agcgtttcga ttcgccgatt ccggtcgcga
      721 gcgcggccat gacctcgggg cggtgcgcat ggttctcgag ctgcggcaac gcgtccgccg
      781 ggaactcggt gtcgccccgc acgcgccccg tcgagtcgat caacgtcacg cggtggccga
      841 tgagggtgcc gagctgaatc gcgatcgcgg gccaggcggt cgagtctgtc gggatcagcc
      901 gcgccaccag ccgggcctcc cgttcgagtc cctccgcggt ctgtagctcg agccggtcgc
      961 gcaggatgcg atcggcggcg atggtgagtc ccagaaccgt ggccgcgacc agcaggctgg
     1021 ccgagaggaa gaaccgggtg gcgaggcgca ttcagcgcgc gcgatcgcgc gcggggggga
     1081 cccggaaccg gtacccgacg ccgcggaccg tctcgacgca atccccggcc gcgccgagct
     1141 tggtgcgcag ccgttgtacg tgcatgtcca cggttcgcgt ctgaatgtcg ggctgcgcct
     1201 gccacacagt ctccagcagg tcggtacgcg attgcacgcg gccggagcgc tccatgagca
     1261 gccgcagcag tttgaattcg gtcgcggtga gctcgatctc gacgtcgttc accgtgacgc
     1321 ggtgggccgc gggatcgagc cgtacgcccg cggcgctgag aatcccgccg gccgccaccg
     1381 gcaccgccgc gaggcggcgc agcaccgcgc ccacccgcag gaccagctcc tgcggcgaga
     1441 acggcttcgc gagatagtcg tcggcccccc gttcgaggcc ggcgatacgg tcctgctctt
     1501 ccctgcgggc ggtgagcagg atgacgccca tgtgccgggt ctcctccgtc cggcgcagct
     1561 cggccaggac gtccaggccg gagcgaccgg gcagcatcag atcgagcacc acgagatcgg
     1621 ggcgctcggc acgcgcccgg gtcagggctt cggtgccgtc gccggcggtg gtcacccgat
     1681 agccggcttt cgccaggtgg taggcgacca ggcccgcgat gtccggctcg tcgtccacca
     1741 cgagcacgcg ttgcggaggg gcggcgtccg cgtcaggggg catgggccgc gtgtcgggga
     1801 ttgcgtggtg cggctgcccg cgcccgcgcc gccgcggcgt cggcgcggaa tgcgggatcg
     1861 gacttgagcg cgtggagcac gccccgcacc atgcgaccct gcgccccggc cgggagcgtg
     1921 aggtggtacg ccacgcgggt ggcgcagcgc tgattctgga ccagtcccat ggtccgcagc
     1981 acccgtaggt gccgcgacac ggtgggctgg ggcagatcga ggatgcccac gaggtccgat
     2041 acgtagagcg tggtcgccgc gaggcagttg agcacgcgca accgcgtcgg ctcggcgagg
     2101 gtcgtgagat agtcggccgg cgacatgtgc gtcgaaggta gcgcggcgac ccgagccttg
     2161 gcaattttcg gctcatcatg cgcttcctcc tgagcatcgt ggtcgcagtc cagcaggcgc
     2221 cgcttcccag tcccgatacc gctcacgtcg tgatcgtcgc caccaccgac gtccacggtc
     2281 gcgtgctggg ctgggactat gtgcgcgacc gggcggcgcg cggcggtctg tcgcgcgcgg
     2341 cggcgatcct cacgacgctg cgcgcccagt atcccggcga ggtgattctg gtggacgcgg
     2401 gcgacctgct ggaggggaat ccgttcgcca agtacttcgg gcgcgtcgag cggcgccgcc
     2461 ccgatccggt ggtggacgcc ctcaacgccc tgcagtacga cgcggcgacg cccggcaacc
     2521 acgacttcga tttcggcctg gatctgctgg cgagtgcgga cagcgacgcc acgttccgct
     2581 acgtcagcgc gaacgtggtg atgaccgtgg ggggcggggg cgggtcgcgg gactccctgc
     2641 tgtttcccgc gacggtggtg atacagcgcg gcccagtgcg cgtggggatc acgggattca
     2701 cgacgccggg cgtgaacgtg tgggaccggc gacagctgca cgggcgggtg cacgtgcggc
     2761 cgatcgccgc cgccgctccg gccgccctgg cggcgctcga gcgcgcgggg gtggacctca
     2821 aggtcgtgct ggttcacagc ggcctggggg aggcgtcgtc ctacgacacg acgggggtcg
     2881 gcccggagaa cgatgccgcg gcgctggcgc gggtgacgcc gcggccggac gtggtcgtgg
     2941 tgggacacac ccaccgcgaa atacgcgact ccgtgatcaa cggcgtccat ttcgtacagc
     3001 cgaggaactg ggcgctgagc ctgtcggtgg tgcacgtctg gct
//
LOCUS       DBNGZW010000587         2230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_109900, whole genome shotgun sequence.
ACCESSION   DBNGZW010000587 DBNGZW010000000
VERSION     DBNGZW010000587.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2230
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_109900"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..>2230)
                     /locus_tag="AC16JC_13890"
     CDS             complement(25..>2230)
                     /locus_tag="AC16JC_13890"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5165836.1"
                     /translation="LLSDRHALVTDFGVAKAVAGSDNLTAVGVTLGTPAYMAPEQAAG
                     DPHPDQRTDVYAVGVLGYELLTGRPPFTGLTPQETLAAHVTQAPEPVEARRSGVSPAF
                     AAVLMRCLAKRPADRWQTADELLAQLERLVARRGSTPTATRAPVGPDVSPSPVLRAFL
                     AVVGASVLLGAGVLVARRSAPEVPQFGRRWPVTIDPGVEIDPSLSPDGRFVAYVAGPL
                     GHTRLQVRQVEGGMPIDLVRDSSGRERFPYWSRDGQQILFQSRRGIEVVPVLGGPSRL
                     VVAAGGASPIPGPVAPDGAHFVYASHDSLFVTALEGQARRLLTTGREMHSFSWSTDGR
                     WIAYVSGNVQYVSSQVLGNIAESGIWVIAADGGTPVRVVDDRSLNVSPAWGTGRALYF
                     VSNRDGGREVYRLELGRTGSPTGPPIRLSTGLSAHGISLSADGTRLAYSVFTETSNVW
                     GVAIPAAGGPVSVSQARPLTAGNQTIEGFAISRDGRWLAFDSNRSGLQQVYRLPIGGG
                     APEQVTRDSVDAFRPSWSPDGLQLAYHSFRGGRRQVFLVEADGGAPTQVTSGADDRRT
                     PEPSPDGRYLLVHTNSYTAPALQLLTRTAAGWSAPRPLPIVLGTDTVAPQAVVVGTWS
                     PDSRFVACACAGLVVIPVAGGQAKRLATPDGGPVAWSDDGRTLYYLHTDSAGAMSVNA
                     IPSTGGASRVVVRFDDPTRPWHRYSLRAKGGRLYFTLGDLQSDIWVTDLEPR"
BASE COUNT          339 a          834 c          781 g          276 t
ORIGIN      
        1 tcctggctcg tcaccaccgg acggtcatcg gggctccagg tcggtcaccc agatatcgct
       61 ctgcagatcg ccgagggtga agtacagccg cccccccttc gcccgcaagg agtagcgatg
      121 ccacggccgc gtcgggtcgt cgaatcggac cacgacgcgc gacgcgcctc ccgtggacgg
      181 gatcgcattc acgctcatgg cgccggccga gtcggtgtgc aggtagtaca gggtgcgccc
      241 gtcgtccgac caggcgaccg gacccccgtc gggagtagcg aggcgtttcg cctggccacc
      301 cgccaccggg atcaccacca ggccggcgca cgcacacgcc acgaaccggc tgtccgggga
      361 ccaggtcccg acgaccaccg cctgcggcgc caccgtgtcc gttccgagca cgatgggcag
      421 cggccggggc gctgaccagc ccgccgccgt gcgcgtgagg agctgcagcg ccggggccgt
      481 atacgagttc gtatggacca gcagatagcg gccgtcgggc gagggctccg gcgtgcgccg
      541 gtcgtccgct ccgctcgtca cctgggtcgg cgctccgccg tcggcctcta ccaggaacac
      601 ctgccgccgc ccgccgcgga agctgtggta ggcgagctgg aggccgtccg gagaccagga
      661 cggccgaaac gcgtcgacgg agtcccgggt cacctgctcc ggtgcgcctc cgccgatggg
      721 aaggcggtac acctgctgca gtcccgagcg gttggaatcg aacgccagcc agcggccgtc
      781 gcgcgagatg gcgaacccct cgatcgtctg attccccgcg gtcagcggcc gggcctgcga
      841 cacggacacc ggtccacccg ccgcggggat cgcgaccccc cacacgttgg aggtctccgt
      901 gaacacggag tacgccaggc gcgtgccgtc cgccgacagg ctgatgccgt gcgcgctgag
      961 accggtcgag agccggatcg ggggtcccgt gggcgagccg gtacggccga gctcgagccg
     1021 gtagacttcg cgtccgccgt cgcgattgga gacgaaatac agcgcgcgcc cggtccccca
     1081 cgcaggactg acgttcaacg accgatcgtc caccacgcgg accggcgtgc caccgtccgc
     1141 ggcgatcacc cagatgccgc tctcggcgat gttgcccagc acctgggagg agacgtactg
     1201 cacgttgccc gacacgtagg cgatccagcg cccgtcggtg gaccaggaga aggagtgcat
     1261 ctcccttccg gtcgtgagga gccgccgggc ctgcccctcc agcgcggtga cgaacagcga
     1321 gtcgtgcgag gcgtagacga agtgcgctcc gtcgggcgcc acgggccccg gaatcggcga
     1381 cgcgccgccc gcggcgacga ccagacggga cgggccgccc agcaccggga cgacctcgat
     1441 gccccggcgc gattgaaaca ggatctgctg accgtcccgg ctccagtacg gaaagcgctc
     1501 ccggccgctg gagtcgcgca cgagatcgat cggcatccct ccctcgacct ggcgcacctg
     1561 cagccgggtg tggcccagcg gacccgcgac gtaggccacg aagcggccgt ccggtgacag
     1621 tgacgggtcg atttcgaccc cggggtcgat ggtgacgggc cagcgccggc cgaactgcgg
     1681 cacttccgga gcgctgcgtc gcgcgaccag cacgccggct ccgaggagca cactcgcccc
     1741 gacgacggcg aggaaggcgc gcaggaccgg ggacggcgaa acgtccgggc ccacgggtgc
     1801 ccgggtcgcg gtcggcgtcg acccccggcg cgcgaccaaa cgctcgagct gcgcgagcag
     1861 ttcgtccgcc gtctgccacc gatcggccgg gcgcttggcc agacagcgca tcagcaccgc
     1921 tgcgaacgcc ggcgagaccc cggagcgccg cgcctccacc ggctccggcg cctgcgtgac
     1981 gtgggccgcc agcgtttcct gcggcgtgag gccggtgaac gggggccgcc ccgtcagcag
     2041 ctcgtagccc agcaccccga ccgcgtacac gtcggtccgc tgatccggat ggggatcccc
     2101 cgccgcctgc tcgggcgcca tgtacgccgg tgtgcccagc gtcaccccca ccgctgtcag
     2161 gttatccgac ccggccaccg ccttcgccac cccgaagtcg gtgacgaggg catgccggtc
     2221 cgacagcagc
//
LOCUS       DBNGZW010000588         8507 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1555091, whole genome shotgun sequence.
ACCESSION   DBNGZW010000588 DBNGZW010000000
VERSION     DBNGZW010000588.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8507
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1555091"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..3893
                     /locus_tag="AC16JC_13895"
     CDS             72..3893
                     /locus_tag="AC16JC_13895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165837.1"
                     /translation="MRSISWIRFAVAGVLGVLAAGASASTAAAKPVRPLRPRTLNLFA
                     SSGLLFQTNRIQCGVQNDGQTCVAFAGSPVGGGGFWPKGTPDQYIFNSGLQIGVVVDP
                     NATFAWHGDTTGAYFFDARGDQLSGEGLTGWYSALNPADVTTWPNGAVVRNGSDPIYN
                     SLLLGQAAISQGDAWVRYWEGNPNLGPRSGPGQHPAGILVDQRALAWNFPSGNEDIVY
                     FVFTFYNVSARSASGAYNNPTIPPELQAEIAAVGDRFQDINEQKFHIEIPDGGYKLTS
                     IYADFAMDADVAVFSQNYGTAFLPFNIGSIYTGTFLPEVGWQFPADIFGPPFAAAPGF
                     IGVKYLKSPKNAQGNDVGLTMFSMTTNGPPHRDPVGVNQLYHYMSGFLGPSDDACSPF
                     TDPAVARARHLCYLATQQSDSRFFQASGPFDLEPGHATSIVVAYIQAAPVNTPGLVIG
                     GDIVPGVPFGGDSIFVDPGKVRLIDRIAGWVGQNDANGDHIIEQDEVTTVPRSLLNKA
                     LIAQSVFDNGFLLPNAPTAPGFYLVPGNNQVTVVWEKSATEQVGDPFFAAASDPTGAL
                     YDPNFRQFDVEGYRIYRGRTSSNLSLVAQFDYAGTSFVDYTGAISDYGDVDGDGLNTG
                     CAPELAVTADCPAFPAETDLSGNVIQVPPGGRTLLASGGIINLITDTAVTGGASGFPK
                     LSNTGVPFAYVDRGVLNSFTYFYAVTAFDVNSVKSGPTSLESPRVTKSVIPRRSSGQF
                     VGASISEPQLVGAKGEPLDPSASLPTLDPATGIFSGKMPPTDQFVASLAAFIPEVLAG
                     GQVDVRIDSIVPGSSATPNVPSPVPALYYVTGAGPTGASQVVIPLQFDCCDADAPITT
                     RYVPVVNSDAAKASVYGGDGTFSLYSSLSIQAPGTWRTTSWGRGSINGAPANSDFNGP
                     RWWTGAANENTNDPNGVLCAPASGGCVLADLSRNAGALPGVSIFHPQSYSSVPSANQR
                     DLEGVTSGVTRAADFRVVWGANGAIDTVFDETHLVPVAFSPKIRASWGILNDSSFVAT
                     AGSRDGKNTLLTWSDPFCVDPMPVVLPAVCAIGGTAPAALMDHARLSPIALSSSSLAG
                     TAALTTTGNGFIFYLNGHFFLMQMAALPTAGTVWHARFYSGSVTGAAGSYEFVPALRP
                     PAVPGLRASFTFTGSTLNTAVTSDSLLQNVHTVPDPYYVTNALEITTNSKVLKFVNVP
                     AKCLIRIFSLSGVLVQLLAVDDPGGGGEATWNLRNRNNQFVASGVYFYQVETPDGKTK
                     VGRFTIVNFAP"
     gene            3904..5037
                     /locus_tag="AC16JC_13900"
     CDS             3904..5037
                     /locus_tag="AC16JC_13900"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HIR5165838.1"
                     /translation="MITKHWQITLCAVALAAGWSVPASAQNQGTQDNTAYGGASGEFL
                     LLGAGARGTALGGAYGALATDVTAMYYNPAGLSQMPRPQVMLSTLNYVANTTYAWGGI
                     AFPLSGGSRAVGVSLGSFGFSDQPIYTIENPDGDGRTYSVRETFVSVSGAQNFSDRFS
                     AGMSLKMINDRLGTARGSAFAVDFGTNFHAMIGERPIRASFVIQNLGSNIRHDGQDLT
                     VGTQREPPLGTVPIPQEPQPARLRSTSWTLPVLFRVGLALDVMAQGMNRVTVLGEFNQ
                     SNTNKPSAGGGLEWAVTNIGNSGFSLAARGSYSIQPDNQTGDLSTGGQTTSQSGGSFT
                     SDGLAVGGGLSYSRGRGQVGFDYAYRNMGVLGGTHFLSFTVGW"
     gene            5209..6291
                     /locus_tag="AC16JC_13905"
     CDS             5209..6291
                     /locus_tag="AC16JC_13905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165839.1"
                     /translation="MFRLFGVLTGLALVGCHKDPLGDLDGTPSAVTLSFAELTLNNGS
                     SGSLTASVVDARAVPLPEGVTFTSRDPAIASVAPDPTYAPVPATSARAVVQATGLGVT
                     YVVATGAGLNDSARVIVLPVNFNGALSSTTPAGGSILSIAGTSLLKFNPATVSVSFPT
                     AGAATLVSKTADTVKVIVPYGAGPGPLTISGITVTYLPGALVSLNTAANVSVTGDFWA
                     GDSSWQTAPDITARLPVVNGTSFFIATTGRPNASKCPEVVLGFGSTGPCMMFRLSLPD
                     TATYSFSVDWQGGASSPDIDVYACSDSTVANFGSACFEDGGGGATGAKPQEISSYTFP
                     AGDHWFVIEVYGGGSSDNIAVTITRP"
     gene            6424..7803
                     /locus_tag="AC16JC_13910"
     CDS             6424..7803
                     /locus_tag="AC16JC_13910"
                     /inference="COORDINATES: protein motif:HMM:NF040439.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HIR5165840.1"
                     /translation="MKTGPVALALTAVLAVGAGRPAQEPAGLSLSAVRSYRPASNQTV
                     VDVFCRVPLLLVSPIGTTGGGAYRFAVSVRDSGGLELVAQSWLTPVREQLLRTRGAST
                     GEHLRFAAGAGRYTIGVTATDSASGRVLRAEAPLSAFTQTPGASDLLLASDFRESSSP
                     TDTAARGGEVRHGSLFFTTSGTPVLTPQASKLAYYLELYPTRAESVQVTGRVLTDSGA
                     QVAAVPLPPEVIGAAGGFATRVVDLAGLPPGRYRFEVTVTGPDSQVTRAAPFGMTGFE
                     TVAEAAAMVESNEWPANLAEAQLDSAYEPLIYLMAAEEQGVYSSLSVEGKRRWLRQFW
                     RRRDPSPGTVRNEERERFYASITEANRRFREGGASAIPGWRTDRGRIFIKYGVPDEVL
                     ERHVQAGVSPYEIWKYARNRSLKYVFMDLTRFGNYKLIYTDDRREPSRPDWQDLLGPD
                     GLKDLQQIQ"
     gene            complement(7807..>8507)
                     /locus_tag="AC16JC_13915"
     CDS             complement(7807..>8507)
                     /locus_tag="AC16JC_13915"
                     /inference="COORDINATES: protein motif:HMM:NF014665.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HIR5165841.1"
                     /translation="HRLVAAIVSTLVVLVAGVTWWERRRRGAPSTEHSAPSWGGYIAV
                     GLLVVQVLLGAVTVKLELPPWTVILHLGTALALFAVLLWIALGAPAGRPGAAALVAAG
                     WGFVTVLFGGLTANLGAATACVGFPLCNGQLVPAGNYLQHIHWSHRVLAYGLVVYLLV
                     WTLRSRRRGAAVGLALVVLQIAVAATMVLRTVPQSLQAAHAAIGAALWGALVVEAVLE
                     SRGRTTEAPAAAGA"
BASE COUNT         1409 a         2828 c         2816 g         1454 t
ORIGIN      
        1 taggacatgc gttgaggcgg tgccgccacc gagcggcacc gcctggcgcg atctcacgct
       61 gaggagtcga catgcgttca atttcgtgga ttcgcttcgc ggtggccggc gtgctcggcg
      121 tgctcgcggc aggggcgagt gcctcgacgg ccgccgccaa gcccgtccgt ccgctccggc
      181 cacgcacgct caacctgttc gccagttccg gactcctgtt ccagacgaac cggatccagt
      241 gcggcgtgca gaacgacggg caaacctgcg tcgcgttcgc cggttcaccg gtgggaggcg
      301 ggggcttctg gcccaagggc acccccgatc agtacatctt caactccggt ctgcagatcg
      361 gtgtggttgt ggatccaaat gcgacgttcg cgtggcacgg ggacacgacc ggcgcctact
      421 tcttcgacgc ccggggcgat cagctctccg gggaagggtt gaccggctgg tacagcgccc
      481 tgaatcccgc cgatgtcacc acctggccca acggcgcggt ggtgcggaac gggagtgatc
      541 cgatctacaa ttcgttgctc ctcgggcaag cggcgatttc ccagggcgat gcgtgggtgc
      601 gctactggga aggcaacccg aatcttgggc cgcgcagcgg tcccggtcag cacccggcgg
      661 gcatcctggt ggaccagcgc gcgctggcgt ggaacttccc cagcggcaac gaggacatcg
      721 tctatttcgt cttcacgttc tataacgtgt cggcccgttc cgcttcgggg gcgtacaaca
      781 acccgacaat tccgccggag ctgcaggccg aaatcgccgc cgtgggcgat cggttccagg
      841 acatcaacga gcagaagttc cacatcgaaa tccccgacgg cggctacaag ttgacgagca
      901 tctacgccga cttcgccatg gacgccgacg tcgccgtgtt cagccagaac tacggcacgg
      961 cattcctgcc gttcaacatc ggatcgatct acaccggcac gttcctgccg gaggtggggt
     1021 ggcagttccc ggccgacatc ttcggtccac cgtttgcggc ggcgccgggg ttcattgggg
     1081 tgaagtacct gaagtcgccg aagaacgccc agggcaacga cgtggggctg acgatgttct
     1141 cgatgaccac gaacggcccg ccccatcgtg accccgtggg cgtgaaccag ttgtaccact
     1201 acatgagcgg gttcctcggc ccgagcgacg acgcctgcag cccgttcacg gaccccgcag
     1261 tggcccgggc acgtcacctg tgctacctgg ccacccagca aagcgactcg cgcttcttcc
     1321 aagcgtccgg tccgttcgat ctcgagccgg ggcatgccac cagcatcgtc gtggcctaca
     1381 tccaggcggc gccggtcaac acgccggggc tggtcatcgg tggcgatatc gtccctggag
     1441 ttcccttcgg tggtgactcg atctttgtgg atcccggtaa ggtccgcctg atcgatcgca
     1501 tcgccggctg ggtcggccag aacgacgcga atggtgacca catcatcgag caggacgagg
     1561 tcaccacggt gccgcgttcc ctgctcaaca aggcgctcat cgcgcaaagc gtgttcgaca
     1621 atggcttcct gcttcccaac gcccccacgg cgccggggtt ctacctcgtg cccggaaaca
     1681 atcaggtcac ggtggtctgg gagaagagcg cgacggagca ggtcggcgat ccgttctttg
     1741 ctgcggccag cgatccgacg ggcgcgctgt acgacccgaa tttccggcag ttcgatgtcg
     1801 agggatatcg gatctatcgc ggtcggacgt cgagcaatct gagcctggtc gcccagttcg
     1861 actacgccgg cacctcattc gtcgactaca ccggcgccat atccgactac ggcgacgtcg
     1921 atggcgacgg gcttaacacc gggtgtgccc cggaactggc tgtgacggcc gattgtcccg
     1981 cgttcccggc cgagacggac ctctcgggca acgtgatcca ggttccgccc ggagggcgga
     2041 cgctgctcgc ctcgggcggc atcatcaacc tgatcaccga cacggcggtg acgggcgggg
     2101 cttccggctt cccgaagctc agcaacaccg gcgtcccgtt cgcctacgtg gatcgcggcg
     2161 tgctgaactc gttcacgtac ttctacgccg tcacggcatt cgacgtcaac tccgtcaagt
     2221 ccggcccgac gtcgctcgaa tcacctcgcg tcaccaaatc ggtgataccg cggcggagct
     2281 ccggacagtt cgtcggggcc tcgatctcgg agccgcagct cgtcggggcg aagggggaac
     2341 cgctggatcc gagcgcctcg ttgccgacgc tggacccggc aaccgggatc ttctccggca
     2401 agatgccccc gacggaccag ttcgtggcca gtctcgcggc gttcatcccg gaggtgctgg
     2461 ccggcggcca ggtggacgtg aggatcgact cgatcgttcc gggctcgtcc gctacaccga
     2521 atgtgcccag tcccgtgccc gcgctgtact atgtgaccgg cgccgggccg accggggcga
     2581 gccaggtggt gattccgctg caattcgact gctgtgacgc cgacgcaccg atcacgacgc
     2641 gatacgtgcc ggtcgtgaat tcggacgccg ccaaggccag tgtctacggc ggggacggca
     2701 ccttctcgct gtactccagt ctgtcgatcc aggcgccggg aacgtggcgg actacgagct
     2761 ggggacgtgg gagcatcaac ggcgcgccgg ccaattcgga cttcaacggg ccgcgttggt
     2821 ggaccggcgc cgccaatgag aacaccaacg atccgaacgg cgtgctctgc gcccccgcct
     2881 cgggcggctg cgtgctggcg gacctgagtc gcaacgccgg tgcgctcccc ggggtgtcca
     2941 ttttccaccc tcaatcgtac agctccgtcc cgagcgctaa tcaacgcgat ctcgagggcg
     3001 tgacctcggg cgtgacgcgg gcggcggatt tccgtgtcgt gtggggcgcc aacggtgcca
     3061 tcgacaccgt gttcgacgag acccacctgg tgccggtggc gttcagcccc aagatccgag
     3121 cttcgtgggg tatcctcaac gactcctcgt tcgtcgccac ggccggctcg agggacggga
     3181 agaacactct gctgacctgg tccgacccgt tctgtgtgga tccgatgccc gtggtgcttc
     3241 ccgctgtttg cgccatcggg ggcactgccc ccgcggccct gatggaccac gccagactct
     3301 cgcccatcgc cctctcgtcg tccagcctgg cggggacggc cgcgctgacc acgaccggga
     3361 acggcttcat tttctacctc aatgggcact tcttcctgat gcaaatggcc gcgctgccga
     3421 cggccgggac ggtgtggcac gcacggttct actcgggatc ggtcaccggc gcggcgggga
     3481 gctacgaatt cgtccccgcg cttcggcccc cggcggtacc cgggctgcgg gcatcgttca
     3541 cgttcacggg atcgacgctc aacaccgccg tgaccagcga ttcgctgctc cagaacgtgc
     3601 acacggtccc ggatccgtac tacgtcacca acgcgctcga gattacgacg aactccaagg
     3661 tcctgaagtt cgtgaatgtg ccggcgaagt gtctgatccg catcttctcg ctgagcggcg
     3721 ttctggtgca gttactcgcg gtcgatgacc ccggtggcgg cggtgaagcc acctggaatc
     3781 tccgcaatcg gaacaaccag tttgtcgcct cgggcgtgta tttctaccag gtcgagacgc
     3841 ccgacgggaa gaccaaggtg ggtcggttca ccatcgtcaa cttcgcgccc tgaggacacg
     3901 accatgatca ccaagcactg gcaaatcacc ctgtgcgcgg tcgccttggc ggcgggttgg
     3961 tccgtccccg cgagcgcgca gaatcaggga acgcaggaca acacggcgta tggcggcgcc
     4021 agcggcgaat tcctgctgct gggcgcgggt gcccgtggta ccgcgctggg aggcgcctac
     4081 ggcgcgctcg cgaccgacgt gacggcgatg tactacaatc ctgccggcct gtcccagatg
     4141 ccacggccgc aggtcatgct gtcgacgctg aactatgtgg ccaatacgac ctacgcctgg
     4201 ggcgggattg cgttccccct ctccggcggt agccgggccg tcggcgtcag ccttggctcg
     4261 ttcgggtttt cggatcagcc gatctacacg attgagaatc ccgacggcga cgggcgcacg
     4321 tactccgtgc gcgaaacgtt tgtttccgtg agtggagcgc agaacttctc cgatcgcttc
     4381 tcggccggca tgtcactcaa gatgatcaac gaccgactgg ggacggcgcg tgggtcggcc
     4441 ttcgccgtgg atttcggcac caacttccac gcgatgatcg gggagcgtcc gattcgggcc
     4501 tcgtttgtca tccagaatct cgggtcgaac attcgccacg acggccagga tctgacggtc
     4561 ggcacgcagc gcgagccgcc gctcgggact gtgccgattc cccaggagcc gcagcccgcg
     4621 cggttgcggt ccacttcctg gacgctgccg gtgctgttcc gcgtgggcct ggcgctcgac
     4681 gtgatggcgc aggggatgaa ccgcgtgacg gtcctcggcg agttcaacca gtcgaacacc
     4741 aacaaaccct ccgcgggcgg gggactcgaa tgggccgtga cgaacatcgg caactcggga
     4801 ttctccctcg ccgcccgggg cagctactcg attcagccgg ataaccagac cggcgacctc
     4861 agcacgggcg gccagacgac atcgcaatcg ggcggatcgt tcaccagcga cggcctggcc
     4921 gtgggcggcg ggctgagcta cagccgaggg cggggtcagg tgggcttcga ctacgcgtat
     4981 cgcaacatgg gagtcctggg aggaacgcat tttctgagct tcacggtcgg ctggtaaggt
     5041 cggtgggtac acctggcgct cggggcacgt gctccgagcg ccaggcggca gtcacagcca
     5101 gtggccgggc cacggaatag cggctcggtt ctgaagccca ggccgacgtg gatcggttcc
     5161 aagcttacac attagagact atccagcgag gctcacatga acggaaacat gtttagactg
     5221 tttggcgtct tgaccgggct tgcgctcgtg gggtgccaca aagatcccct gggcgatctc
     5281 gatggcacgc cgagcgcggt gaccctgagc ttcgcggagc tcaccctgaa caacgggagc
     5341 tcggggtcgt tgacggcatc ggtggtcgac gcgcgagcag tgccgcttcc cgagggggtg
     5401 acgttcacct cgcgtgaccc ggccatcgcg agcgtggcac cagacccgac atacgctccc
     5461 gtccccgcca cgtccgctcg agccgtggtc caggcgacgg gcctgggcgt cacgtacgtc
     5521 gtggcgaccg gtgcggggct gaacgattct gccagagtca ttgtgctgcc ggtgaatttc
     5581 aatggtgcgc tgtcgagcac gacgcccgcc ggcggatcaa tcctgtcgat cgccggcact
     5641 tcgctcttga agttcaatcc tgccaccgtg agcgtgagct tcccgacggc cggcgcggcg
     5701 acactcgttt ccaagaccgc cgataccgtg aaggtcattg tcccctacgg tgccgggcca
     5761 ggtcccctga ccatcagcgg gatcaccgtc acgtacctgc cgggtgcgtt ggtgagcctg
     5821 aatacggcgg cgaacgtgag cgtgaccggc gacttctggg cgggggacag ctcctggcaa
     5881 accgcgcccg atatcaccgc acgtcttcca gtggtgaatg gcacgagttt cttcatcgcc
     5941 acgaccgggc ggccgaacgc ctccaagtgc ccggaggttg tcctgggctt cggatccacc
     6001 gggccctgca tgatgttccg tctcagtctt ccggacacgg ccacctacag cttcagcgtt
     6061 gactggcagg gcggcgcgag ctcgccggac atcgacgtat atgcctgctc cgacagcacg
     6121 gtcgcgaatt tcggttcggc gtgctttgaa gacggtggtg gcggcgccac aggtgccaag
     6181 ccgcaggaga tcagctcgta cacgttccct gcaggcgatc actggttcgt catcgaggtg
     6241 tacggtggag gctcgtccga taacatcgcc gtgacgatca cgcgcccgta accgagcgag
     6301 agcgcacagg ggaaccaggt acatggggac cacttgccac cgcaacttgg gggcattggg
     6361 gggtaccaac ttcctgagtt ttggcgtcgg ctggtagccc acaggaaggt ggtcgtggaa
     6421 aaagtgaaga caggaccagt cgcgctggca ctcacggccg tcttggccgt gggtgccggc
     6481 cgacccgcgc aggaacccgc ggggctgagc ctgagtgccg tgcgctccta ccggccggcc
     6541 tccaaccaga cggttgtcga cgtattctgt cgcgtccccc tgctgctcgt gagtccgatc
     6601 gggaccacag gcggcggggc gtaccgtttt gccgtgtcgg tgcgcgactc agggggtctg
     6661 gagctcgtcg cgcaatcatg gctcacgccg gtgcgcgaac agctactacg gacccgcggc
     6721 gcgtcgacgg gggagcatct gcggtttgcg gcgggggcgg gccgctatac catcggggtg
     6781 acggcgaccg attccgccag cgggcgcgtg ctgcgggctg aagcgccgct gtcggcattc
     6841 acacagactc cgggagcatc ggacttgctg ctggcgagcg acttccgtga gtcgagctct
     6901 ccgaccgaca cggcggcacg cggcggcgaa gtgcgtcacg gttcgctgtt cttcacgacc
     6961 tcaggtacac cggtgctcac gccgcaggcg tccaaactgg cctattatct ggagctgtat
     7021 cccacgcgag cggaaagcgt acaggtgacg ggtcgggtcc tcaccgactc cggcgcgcag
     7081 gtcgcagccg ttccacttcc tcctgaagtg atcggtgcgg ccggggggtt cgctaccagg
     7141 gtcgtcgacc tggcgggact tccgcccggc cggtatcgct tcgaggtgac cgtgaccggt
     7201 cccgactctc aggtaacccg tgcggcgccg ttcgggatga ccgggttcga aacggtcgcc
     7261 gaggcggcgg ccatggtgga gtcgaacgag tggccggcga atctcgcgga ggcgcagctc
     7321 gactcggcct acgagccgct gatctacctc atggccgcag aggagcaggg ggtgtactcc
     7381 tccctgtcgg tcgaggggaa gcgcagatgg ttgcggcagt tctggcggcg ccgcgatccg
     7441 tcgccgggaa cggtacgcaa cgaagaacgg gagcgcttct acgcatccat caccgaggcg
     7501 aaccgtcgct tccgcgaggg cggggcgtcg gcgattcccg ggtggcgcac cgaccggggc
     7561 cgcatattca tcaagtacgg ggtgcctgac gaggtgctgg agcggcacgt gcaggcgggt
     7621 gtgtcgccct acgagatctg gaagtacgcg cggaaccgga gcctgaagta cgtattcatg
     7681 gacctgaccc ggttcggtaa ctacaagctg atctacaccg acgaccggcg cgagccgagc
     7741 cggcccgact ggcaggacct gctggggccg gacgggctga aggacctgca gcagatccag
     7801 tgacgctcag gccccggcgg ccgccggggc ttccgtcgtt cgtccccggc tttccaacac
     7861 cgcctccacc accagcgccc cccacagcgc cgcgccgatc gccgcatgcg cggcctgcag
     7921 cgactgcggc acggtgcgca gcaccatggt cgcggcgacc gcgatctgga gcacgacgag
     7981 ggcgagtccc accgccgccc cgcgccgccg tgagcgcagc gtccacacca gcaggtagac
     8041 aaccaagccg tacgccagca cccggtgcga ccagtggatg tgctgcaggt aattgcccgc
     8101 cgggacgagt tggccattgc acagcgggaa tccgacgcac gcggtagccg ctccgaggtt
     8161 ggcggtcaat ccgccgaaca gcaccgtgac gaagccccat ccggcggcca ccagcgcggc
     8221 cgcgcccgga cgcccggccg gagcgccgag tgcgatccag agcaacacgg cgaacagcgc
     8281 cagggctgtc cccagatgga ggatgaccgt ccagggcggg agctccagct tcaccgtgac
     8341 tgccccgagc agcacctgca cgacgagcag cccgaccgcg atgtacccac cccagctggg
     8401 agcggaatgc tccgtgctcg gtgctccgcg cctccgcctt tcccaccacg tcactcctgc
     8461 caccagcacc accagcgtcg acacgatcgc cgccacgagg cggtgat
//
LOCUS       DBNGZW010000589         2094 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_54565, whole genome shotgun sequence.
ACCESSION   DBNGZW010000589 DBNGZW010000000
VERSION     DBNGZW010000589.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2094)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_54565"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(141..2003)
                     /locus_tag="AC16JC_13920"
     CDS             complement(141..2003)
                     /locus_tag="AC16JC_13920"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005911790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5165842.1"
                     /translation="MSVRRHGPPSRLSPGEKPPTWAQRLEALRHVPPLLRLVFQTHHG
                     YTVAILALRIVRAFIPVAVLWIGKLIIDGVIAAIAVAHAGNPVHWWTLGGLIGLELGI
                     AIVGEALARASSLLESLLGDLFANRISVRLMRHAATLDLAQFEDPDTYDHLERARRQT
                     VGRIGLFALLLSTAQDLVTLASLAGVLLVQLPWLLLLLALAVVPSFLGEAHYASLGYS
                     LLFRWTPERRLLDYLRYMGASDESAKEVKLFGLSNFLVGRYARLSDEFYVANKKLAVR
                     RNVVSTALVTLGTLGYYAAYAVIIYLTVLGRYTIGSLTFLAGSFRSSRDLIQRILLSL
                     SNIYEQSLYLGDLFTFFDVQPLVVSKPDGRAVPKPIRTGFEFRDVGFRYPGSDRWAVR
                     HLTFTVGPGERIALVGENGAGKTTLVKLLARLYDPDEGRILLDGVDLRDYDLTSLRRN
                     IGVIFQDFVRYDFILKENIGVSEIERLEDETRIEEAAHRSHADAVAGRLAGGYDQMLG
                     RRFEGGVDLSGGEWQKVALARAYLREAQVLILDEPTASLDARAEYEVFLRFAELTKGK
                     MAVLISHRFSTVRMADRILVLQWGELVDQGTHEELVARGGLYAELFSLQAAGYR"
BASE COUNT          371 a          690 c          717 g          316 t
ORIGIN      
        1 tccccgcggg gcgttacgtg ctccattaca ggaccgacgg atcgcacgcc tatggggact
       61 ggaacgacga tccgccggac gatccggagg gatggggcat cgtggtcacg cgagccccgt
      121 agtcacgcgc atgaccgccg ctagcggtat ccagcggcct ggaggctgaa caactcggcg
      181 tagagtccgc cgcgcgccac cagctcctcg tgcgtgccct gatccaccag ctcgccccat
      241 tgcagcacca ggatgcggtc ggccatgcgc acggtcgaga agcggtgtga aatgagcacc
      301 gccatctttc ccttcgtcag ctccgcgaag cgcaggaaca cctcgtactc ggcgcgggcg
      361 tcgagcgacg cggtgggctc atccaggatc agcacctgcg cttcccgcag gtaggcgcgc
      421 gccagtgcga ccttctgcca ttcgccgccg gagagatcca cgccgccttc gaagcgccgg
      481 cccagcatct ggtcgtagcc gccggcgagc cgcccggcca cggcgtcggc atggctgcgg
      541 tgcgccgcct cttcgatccg ggtctcgtct tcgagtcgct cgatctcgct caccccgatg
      601 ttctccttca ggatgaagtc gtagcgcacg aagtcctgga agatcacgcc gatgttgcgg
      661 cggaggctcg tcaggtcgta atcgcggagg tccaccccgt cgagcagaat ccgtccctcg
      721 tcgggatcgt acaaccgtgc gagcagcttc acgagggtgg tcttcccggc gccgttttcg
      781 ccgaccagcg cgatccgctc cccggggccc accgtgaagg tcaaatggcg taccgcccag
      841 cggtccgagc ccggataccg gaatccgacg tcgcgaaact cgaatcccgt ccggatcggt
      901 ttcggtaccg cccgcccgtc cggcttcgac acgacgagcg gttgcacgtc gaagaacgtg
      961 aacaggtcgc ccaggtagag gctttgctcg tagatgttcg acagcgagag caggatgcgc
     1021 tggatcaggt cgcggctcga acggaacgag cccgcgagga aggtcagcga gccgatggtg
     1081 tagcggccca gcaccgtgag gtagatgatc accgcgtagg ccgcgtagta gccgagcgtc
     1141 ccgagcgtga ccagggcggt cgacacgacg ttgcgccgca cggcgagctt cttgttcgcc
     1201 acatagaact cgtcggacag cctggcgtaa cgccccacca ggaaattcga gagcccgaac
     1261 agcttcactt ccttggccga ttcgtcggag gcgcccatgt aacgcaggta gtcgagcagg
     1321 cggcgctccg gggtccagcg gaacagcagg gagtagccga gcgacgcgta gtgcgcctcc
     1381 cccaggaacg acgggaccac cgccagggcg aggagcagga gcagccaggg caattgcacg
     1441 agcaacaccc cggccagcga ggcgagggtc acgaggtctt gcgccgtcga gagcagcagc
     1501 gcgaagagcc cgatacggcc caccgtctgg cggcgggcgc gttccaggtg atcgtaggta
     1561 tcgggatctt cgaactgggc gaggtcgagt gtcgcggcgt gccgcatcag gcgcacgctg
     1621 atgcggttgg cgaacaggtc gccgagcagg ctctcgagca gcgacgaagc gcgggcgagc
     1681 gcctcgccca cgatggcgat gcccagctcc aggccgatca gtccgccgag cgtccaccag
     1741 tgcaccgggt tgccggcgtg cgccacggcg atggccgcga tcacgccgtc gatgatgagc
     1801 ttgccgatcc acagcaccgc caccggaatg aacgcgcgga cgatgcgcag cgcgaggatc
     1861 gccacggtgt agccgtgatg ggtttggaac acgaggcgca gcagcggcgg cacgtgacgc
     1921 agcgcctcga ggcgttgggc ccaggtgggg ggcttctcgc ccggggacaa gcgcgatggg
     1981 gggccgtgtc gtcgaacgct cacgccgtga aaagtaccgc ctccttgcgt cttgtgtccc
     2041 ggtggtgcag cgttgctcgc gcaatggaca tgcaaccacg accggggagg attc
//
LOCUS       DBNGZW010000590         6921 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_806549, whole genome shotgun sequence.
ACCESSION   DBNGZW010000590 DBNGZW010000000
VERSION     DBNGZW010000590.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6921)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_806549"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..540)
                     /locus_tag="AC16JC_13925"
     CDS             complement(<1..540)
                     /locus_tag="AC16JC_13925"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HIR5165843.1"
                     /translation="MSTWAVILAGGSGTRFWPLSTPERPKQLLPLAGDRPLLSQAVRR
                     LEGLVPPDRVLILTGPFLVERVTALVPEVPAAQVLAEPRAASTAPALVWGAHWIARRD
                     PDAQMLSLHADWAITDEAAFRSTAARALAVAREHDVLVTVGVKPTRPESAYGYIVPGR
                     PLGRFARRVQRFVEKPTAAR"
     gene            complement(537..947)
                     /locus_tag="AC16JC_13930"
     CDS             complement(537..947)
                     /locus_tag="AC16JC_13930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5165844.1"
                     /translation="MVPPAAPRTRRLLVVDDEPHIGLLLRLQLESRGYDIRLARSLFE
                     ARGAMKDEAGPPDAIMLDIHLPDGSGIEFLRELRDRPATAEIPVMILTAEGEERVLAE
                     ARQMGAKTITKPFSPSKLRTLVATLLGDAAEDAG"
     gene            complement(980..1333)
                     /locus_tag="AC16JC_13935"
     CDS             complement(980..1333)
                     /locus_tag="AC16JC_13935"
                     /inference="COORDINATES: protein motif:HMM:NF015232.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HIR5165845.1"
                     /translation="MPTIRELVQAIARRTGVDAVVVVGSDGLPIDSVSGDGVDPDSVA
                     ALLPSVIRGLSELGGAGGRGDFSTGVLEFDTGLAVVSVLHADAMLVVLVRPDTNVGTL
                     LFDLRRHRTAIAGLL"
     gene            1449..1994
                     /locus_tag="AC16JC_13940"
     CDS             1449..1994
                     /locus_tag="AC16JC_13940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165846.1"
                     /translation="MQAGSLLVVAALFVAACGNNTGLGNANIPNVVDTISLYALDRTP
                     ISLPSAYVIRGNSVVRTDLVPGFDFAFNITAAGQAVLLPTGALGLGVASGVQLQTVSF
                     TALTQAPTNRYVDSLPVPIDSGTVAVVHSRPFSDFCGVVFYYGKVEVLAIDTTARRVD
                     LQILTDQNCGFRGLEPGLPRR"
     gene            1999..3294
                     /gene="serS"
                     /locus_tag="AC16JC_13945"
     CDS             1999..3294
                     /gene="serS"
                     /locus_tag="AC16JC_13945"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012818168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR5165847.1"
                     /translation="MIDLRLLRSAPAEMRAALARRGDPGALELLDALQELDARRRTLT
                     TALDALKAERNEAARADAQLVKQQGSLPPETVAARRALGQRIGAAEGEVREVEAALEA
                     KALYVPNLPAGDVPDGDATANRVVRSWGTPRPGGPDVRPHWEIGERLGLLDLPRGAKL
                     SGSGFPVLVGAGARLARALIQFMLDLHTREHGYVEVEPPFLVRREIMQGTGQLPKFEA
                     DAYRTTEDDMFLVPTAEVPVTNLYRDEILDGARLPLGHTAYTPCFRREAGAAGKDTRG
                     LLRVHQFDKVELVRFVRPADSGPEHERLTGHAEAVLQRLELPYRVVLLAAGDTGFAAA
                     KTYDLEAWAPGVGAWLEVSSSSSFTDFQARRAGIRYRAAKGEKPEFVHTLNASGVALP
                     RTIAALLESRQAADGSVSIPPVLVPYVGTDRITAPSSGA"
     gene            3287..4456
                     /locus_tag="AC16JC_13950"
     CDS             3287..4456
                     /locus_tag="AC16JC_13950"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5165848.1"
                     /translation="MRSDALRRSGPPVVAGLALLFGALSVGSAWVVARHLREDARDTS
                     RLFGRVFAGLNDPSPDAATEALFDLAAQVRSLGIPIAVTDSAGHVTAMENAPPGAGRD
                     SAHLRAWVAELDAVNPPVSGPGIGTVHYGTLAITRRLALVAGLQVVVLFALVLLAVWA
                     YRERLGAVRDRMWVAMARESAHQLGTPLMSLTGWIAQLREHPQMRAAEVAPHLEADVE
                     RLERVARRFERIGRPPRSEPVGLGAVAERVVTYFLPRLPTRANAVTLNLTAAGPGPTI
                     AGDPVLLEWALEAIVKNAVDALSGRGGHVAVLVEGTDEQATVSVRDDGPGIPPGRRSK
                     VFEPGETSKPGGWGLGLPLARRIVEQQHGGRLRYHPGNPGSLFVLEFPLASGDRA"
     gene            4453..6735
                     /locus_tag="AC16JC_13955"
     CDS             4453..6735
                     /locus_tag="AC16JC_13955"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR5165849.1"
                     /translation="MTLDLNPAQRRAVEHPGGPLLVLAGAGSGKTRVLTARIAHLIRA
                     AGVRPDRIFAVTFTNKAAGEMRERVATLLGADPRGLWIGTFHSLSARLLRREAPLLGF
                     GPNFTIYDEDDSQAVLKRMLEQRELSPKAYPPRALHALISGAKNRMLTPEEYLASADT
                     PLTRVAADCYRDLGEALLRANAMDFDDLLLFPLRLFREHPERLVWWQGRFDHVLVDEF
                     QDTNAAQYRLVRALAQRHGNLCVVGDDDQAIYGWRGADVRHMLAFQNDFPGTTLIKLE
                     QNYRSTQIILDAANGVIAENRERLGKTLFTAVPGGTPVVLLTAADERDEAEWIAGELA
                     SQAQHSDLPYEAMAVLYRTNAQSRPLEEAFRMRGVPYRLVGAVSFYERREVKDLLAYL
                     RLIANPADDQAFSRVANVPRRGIGDTSLTLLAETARRWGKPLLETARAADRVEGLRPG
                     LRETLRNFASLVDRLREAVGTADPATALETVILRIGYDPWLAEEGVEGVERLENVREL
                     VAGAAAWAEVAEATGAGAGEGEGDDGGGTLIERYLTQSALVTPTDEFGGADAAGVTLL
                     TVHMAKGLEWPIVALTGLEDGLFPLARTAGEPGGLEEERRLCYVGLTRARERLYLSWA
                     RTRYRNGRLEQSESSRFLEALPATVYEERSTTPAWSRATYERRTPSGYSSPYRAARRR
                     EVEEVEPAFDVEESQDAPRYVKGERVRHRKFGGGVVREVSGVGRNLKVTVEFDDSVVG
                     SKQLLVAYVALEREWDGEGA"
     gene            6732..>6921
                     /locus_tag="AC16JC_13960"
     CDS             6732..>6921
                     /locus_tag="AC16JC_13960"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR5165850.1"
                     /translation="MSVSREEVEKIARLAELHVDDAALPGLVDQLSRILDYVAQIAGV
                     AASESAQPFVPGPAAIRFR"
BASE COUNT          980 a         2378 c         2567 g          996 t
ORIGIN      
        1 gcgggccgcg gtcggcttct cgacgaaccg ctgcacgcgt cgggcgaacc gcccgagcgg
       61 acgtcccgga acgatgtagc cgtaggccga ttcggggcgc gtgggcttaa cgcccaccgt
      121 caccagcacg tcgtgctcgc gcgctacggc gagcgcccgc gccgcggtgc tgcgaaacgc
      181 cgcctcgtcg gtgatggccc aatcggcatg gagggagagc atctgcgcgt ccgggtcgcg
      241 ccgcgcgatc cagtgtgcgc cccacaccag cgcgggggcg gtcgacgcgg cccgcggctc
      301 cgccagcacc tgcgccgccg gaacctcggg tacgagcgcc gtcacccgct ccaccaggaa
      361 cgggccggtg aggatcaaga cgcgatccgg cggcaccagt ccctcgagcc ggcgcacggc
      421 ctgggagagg agcgggcggt cgccggccag cggcaggagc tgcttgggac gctccggggt
      481 ggagagcggc cagaatcgcg tccccgagcc tccggccagg atgaccgccc aggtgctcac
      541 ccggcgtcct ccgccgcgtc tcccagcagc gtcgcgacca gcgtgcgcag cttggacggg
      601 ctgaacggtt tggtgatggt cttggcgccc atctggcggg cctcggccag gacgcgctcc
      661 tcgccctcgg ccgtcaggat catcaccggg atctccgccg tcgcgggccg atcccgcagc
      721 tcgcgcagga actcgatgcc cgagccgtcg gggaggtgga tgtccagcat gatcgcgtcg
      781 ggcgggccgg cctcgtcctt catcgcgccg cgcgcctcga acaacgagcg ggccaggcgg
      841 atgtcgtagc cccgcgactc gagctggaga cgcaacagca ggccgatgtg gggctcgtcg
      901 tcgacgacga ggaggcgtcg cgtccggggg gcggcgggag ggaccacggg taccgggggg
      961 ccgggagagg gaaagtggct cagagcagcc cggcgatggc cgtgcgatgg cggcgcaggt
     1021 cgaagagcag cgtgcccacg ttcgtgtcgg ggcgcaccag caccacgagc atcgcgtcgg
     1081 catggagcac cgagacaacc gccagcccgg tgtcgaactc cagcacgccg gtggagaagt
     1141 cgccccgtcc gccggcgccg ccgagctcgc tcaagccgcg gatcaccgag ggcagcagcg
     1201 cggcgacgct gtcggggtcc accccgtcac cgctcaccga gtcgatcggc aggccgtcgg
     1261 atcccaccac gacgacggca tcgacgccgg tgcggcgggc gatcgcctga acgagctccc
     1321 ggatcgtcgg catacgggcg cgaagctagg acgcgctgcg ggggaagtca agcattgata
     1381 gtcggttaag ggttgacccc ccgcgcggcg cgaggatatc ttgacgccac tccatgcgcc
     1441 aacgtcgtgt gcaagcggga tccctcctcg tggtggcggc gctgttcgtc gccgcctgcg
     1501 gcaacaacac gggactgggg aatgcgaaca tcccgaacgt ggtggacacg atctcgctct
     1561 acgcgctgga tcgcacaccg atctccctgc cgtcagcgta cgtgatcagg ggcaattcgg
     1621 tagtgcgcac ggatctggtc ccggggttcg acttcgcctt caacatcacc gcggccggcc
     1681 aggcggtgtt gctgcccacc ggtgcgctgg gtcttggcgt cgcctcgggg gttcagctgc
     1741 agaccgtctc gttcacggcg ctcacccagg cgcccacgaa ccgatacgtc gattcgctgc
     1801 cggttcccat cgacagcgga acggtggcgg tggtgcactc ccgtcccttc tccgatttct
     1861 gcggggtcgt gttctactac gggaaggtcg aggtgctcgc catcgacacg acggcgcggc
     1921 gcgtcgacct ccagatcctg acggatcaga actgcggctt tcgcggtctc gagcccggcc
     1981 tcccacgccg gtagtcccat gatcgacttg cggctcctgc gcagtgcccc cgcggagatg
     2041 cgggcggcgc tggctcggcg tggcgacccc ggcgcgctgg agttgctcga tgcgctgcag
     2101 gagctcgacg cgcggcgtcg caccctcacg accgctctcg acgcgctgaa ggcggagcgc
     2161 aacgaggccg cgcgcgccga cgcgcagctc gtgaaacagc aggggtcgct ccctcccgag
     2221 accgtggccg cgcgccgcgc gctgggtcag cgcatcggcg ccgccgaggg cgaggtacgg
     2281 gaggtcgaag ccgccctcga ggcaaaagcc ctctacgtcc cgaacctgcc cgcgggtgac
     2341 gtgcccgacg gcgacgccac cgcgaaccgc gtggtgcgca gctgggggac gccccggccc
     2401 ggcggtccgg atgtccggcc gcactgggag atcggtgagc ggctcggact gctcgatttg
     2461 ccgcgcggcg cgaagctgtc gggctcgggt ttccccgtgc tggtgggggc gggcgcccgg
     2521 ctcgcccgcg cactcatcca gttcatgctg gacctgcaca cgcgtgagca cggctacgtg
     2581 gaggtggagc cgcccttcct ggtacgccgt gagatcatgc agggcaccgg acagctgccg
     2641 aagttcgagg ccgacgccta ccggacgacc gaggatgaca tgtttctcgt gccgaccgcg
     2701 gaggtgccgg tgaccaacct ctaccgggac gagatcctgg acggcgcccg gctgccgctc
     2761 ggccatacag cgtatacgcc ctgcttccgg cgcgaggccg gggcggccgg gaaggacacg
     2821 cgcggcttgc tgcgcgtgca tcagttcgac aaggtcgagc tggtgcgttt cgtgcggccg
     2881 gccgactcgg gccccgagca cgagcgcctc accggccacg ccgaagcggt gctgcaacgt
     2941 ctcgagctgc cgtaccgtgt cgtgctgctc gccgctgggg acaccgggtt cgccgccgcc
     3001 aagacctacg acctcgaagc ctgggcgccc ggtgtgggcg cctggctcga ggtgtcgagc
     3061 tcctcgagct tcaccgactt tcaggcgcgg cgcgcgggga ttcggtaccg cgcggccaag
     3121 ggcgagaagc cggagttcgt gcatacgctg aatgcctcgg gagtcgcgct gccgcgcacc
     3181 attgcggccc tgctcgagag ccggcaggcg gcggacggca gcgtctcgat tccgcctgtg
     3241 ctggtgccgt acgtcggcac cgaccgcatc accgcgccgt ccagcggtgc gtagcgacgc
     3301 cctgcgtcgg tccgggccac ccgtggtggc cggcctggcg ctgctcttcg gcgcgttgtc
     3361 ggtcgggtcc gcctgggtcg tggcgcgcca cctgcgggag gatgcgcgcg atacgagccg
     3421 gctgttcggc cgtgtgttcg ccggcctgaa cgatccaagc cccgacgcgg cgaccgaagc
     3481 gctgttcgac ctcgcggcgc aggtgcgcag cctcggtatc cccatcgccg tcaccgacag
     3541 cgccgggcac gtcaccgcga tggagaacgc ccctccgggc gcggggcgcg acagcgcgca
     3601 cctccgggcc tgggtcgccg agctcgatgc cgtcaacccg ccggtctcgg gaccggggat
     3661 cggcacggtc cattacggca cgctggccat cacccgccgt ctggcgctgg tcgccgggct
     3721 ccaggtcgtg gtgttgttcg ccctcgtcct gctcgccgtc tgggcgtatc gcgagcggct
     3781 cggggcggta cgcgaccgca tgtgggtggc gatggcccgg gaatcggccc atcagctggg
     3841 gacgccgctc atgagcctca cgggctggat cgcccagctg cgggagcatc cccagatgcg
     3901 ggccgcggag gtcgctcccc acctcgaggc cgacgtggag cgattggagc gggtggcccg
     3961 gcgcttcgag cgcatcgggc gcccaccgcg cagcgagccg gtcggtctcg gggccgtggc
     4021 cgaacgggtg gtgacctact tcctgccgcg gctcccgacc cgggccaacg cggtcacctt
     4081 gaacctcacg gccgccggac cggggcccac gatcgccggc gacccggtgc tgctcgagtg
     4141 ggcgctcgag gccatcgtga agaacgccgt ggatgcgctc tcgggccgcg gcgggcacgt
     4201 tgccgtgctg gtggagggca ccgacgagca ggcgacggtc agcgtgcgcg acgacgggcc
     4261 ggggattcca ccgggacgcc gcagcaaggt gttcgagccg ggtgagacga gcaaaccggg
     4321 cgggtggggc ctcggactgc cgctcgcccg gcggatcgtg gagcaacaac acggcgggcg
     4381 gttgcgctat catcccggga atcccggtag cctgttcgtc ctcgaattcc cgctcgcgtc
     4441 gggagatcgc gcgtgacgct cgacctcaat cccgcacaac gccgcgccgt cgagcatccc
     4501 ggcggtccgc tgctcgtgct cgccggtgcg ggctccggga agacgcgggt gctcaccgcc
     4561 cggatcgcgc atctcatccg ggcggccggc gtgcgacccg accggatctt cgccgtcacg
     4621 ttcaccaaca aggccgcggg cgagatgcgc gagcgggtcg ccacgctgct cggcgccgat
     4681 ccccgcggtc tatggatcgg cacattccac tcgctcagcg cccggctgct gcggcgcgag
     4741 gcgccgctgc tcgggtttgg cccgaacttc acgatctacg acgaagatga ttcccaggca
     4801 gtgctcaagc ggatgctcga gcaacgcgag ctgtcgccca aggcgtatcc gccgcgcgcc
     4861 ctgcacgcgc tgatctccgg tgccaagaac cggatgctga cgcccgagga gtacctggcg
     4921 tcggccgaca cgccgctgac gcgggtggcg gcggactgct accgcgacct gggggaggcg
     4981 ctgttgcgcg ccaacgccat ggatttcgac gacctgctgc tgttcccgct gcggctcttc
     5041 cgggagcacc ccgagcggct ggtgtggtgg cagggtcgct tcgatcacgt cctggtggac
     5101 gaattccagg ataccaacgc cgcgcagtac cgattggtgc gcgccctcgc ccagcggcac
     5161 ggcaacctgt gcgtcgtggg cgacgatgat caggcgatct acggatggcg cggggctgac
     5221 gtgcggcaca tgctggcctt ccagaacgat tttccgggca cgacgctgat caagctcgag
     5281 cagaactacc gctccacgca gatcatcctc gacgcggcca acggcgtcat cgccgagaac
     5341 cgcgagcggc tcggcaagac gctgttcacc gccgtcccgg gggggacgcc ggtcgtgctc
     5401 ctgaccgcgg cggacgagcg cgacgaggcc gaatggatcg cgggggaact cgccagccag
     5461 gcccagcact cggacctgcc ctacgaggcc atggcggtgc tgtaccgcac caacgcgcag
     5521 tcgcgtccgc tcgaggaggc gttccggatg cgcggcgtgc cctaccggct ggtcggggcg
     5581 gtgagtttct acgagcgccg cgaggtgaag gatctgctcg cctacctgcg gctcatcgct
     5641 aatccggccg acgatcaggc gttcagccgg gtcgcgaacg tgccgcgccg cggcatcggc
     5701 gacacctcgc tcacgctcct ggcggaaacg gcacgccgct ggggcaagcc gctgctcgag
     5761 acggcgcgcg cggcggatcg cgtggaaggg ctgcggcccg ggcttcggga aacgctgcgc
     5821 aacttcgcct cgctcgtcga ccggctgcgc gaggcggtgg gaacggccga tccggcgacc
     5881 gcgctcgaga cggtgatcct gcgcatcggc tacgacccct ggttggcgga agagggagtg
     5941 gaaggcgtcg agcggttgga gaacgtgcgc gagttggtgg ccggcgcggc ggcctgggcc
     6001 gaggtggcgg aggccacggg ggcgggggcg ggggaggggg agggggacga cggcggcggc
     6061 acgctcatcg agcgttatct cacccagtcg gcgctggtca cgccgaccga cgagttcggc
     6121 ggcgccgacg cggccggggt gacgctgctg accgtccaca tggcgaaggg cctcgagtgg
     6181 ccgatcgtgg cgctgacggg tctcgaagac ggcctcttcc cgctcgcccg cacggcgggg
     6241 gagccgggcg gcctcgagga ggagcgccgc ctgtgttacg tgggtctcac ccgcgcccgc
     6301 gagcggctgt atctctcctg ggcgcggacc cggtaccgca acggccggct cgagcagtcg
     6361 gagtcgtcgc gctttctgga ggcgctcccg gcgacggtct acgaggaacg gagcacgacg
     6421 ccggcctgga gtcgtgcgac gtacgagcgc cgcactccct ccggatattc cagtccgtac
     6481 cgcgccgcac ggcgacggga ggtggaggag gtggagccgg cgttcgacgt ggaggagtcg
     6541 caggacgcgc cgcgctacgt gaaaggggag cgcgtgcgcc atcgcaaatt cggcggtggg
     6601 gtcgtgcgcg aggtctcggg ggtcgggcgc aatctcaagg tgaccgtcga gttcgacgac
     6661 agcgtcgtgg ggagcaagca actgcttgtg gcgtacgtcg cgctcgagcg cgagtgggat
     6721 ggggaggggg catgagcgtt tcgcgggagg aagtcgaaaa gattgcccgg ctggccgagc
     6781 tgcacgtgga cgatgcggcg ctgccgggcc tggtggacca gttgtcgcgc atcctggatt
     6841 acgtggccca gatcgcgggc gtcgccgcca gcgagtcggc gcaaccgttc gtgcccgggc
     6901 ccgcggcgat ccggttccgg c
//
LOCUS       DBNGZW010000591         4472 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1147323, whole genome shotgun sequence.
ACCESSION   DBNGZW010000591 DBNGZW010000000
VERSION     DBNGZW010000591.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1147323"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="AC16JC_13965"
     CDS             <1..1313
                     /locus_tag="AC16JC_13965"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR5165851.1"
                     /translation="VAMTLPMAVLVAVLHTMSRLVADSEVTAMTANGISVGRIARPVL
                     LWAAVVAILAFLWNDQLLPRSNHRLRTLLVDIQRKKPSLALKEQVINEVVPGQFFLRA
                     ARIDPATNRLRDVTIYDLGDADRRRIITADSGQMAYTPGQTDLYLTLREGDIQEVRRG
                     QDAQFNRTFFHTNFIRVRNVSNQLVRTEEDTYRGDREMTVCEMAQVVRSAGNDARIAH
                     RQGDATVRRDLRRLAGLAPVPAPVADAPADTAPGVFCRWLGRLTSWLLPASAQAAQTP
                     SPAVSVPVTPPAPPVVSQVVLVTPGGISAEQDGAESARRRAAVYLVEIHKKLAIAAAC
                     FVFAVLGVPVALRFPRGGAGLVIGVSVVVFAVYYVGLIVGEDLGDRLIVSPFLSMWVP
                     NLLFGTLGLLGIWVMRRASPTARGGDWSDLKDAIVGLFRRRAHA"
     gene            1313..2383
                     /locus_tag="AC16JC_13970"
     CDS             1313..2383
                     /locus_tag="AC16JC_13970"
                     /inference="COORDINATES: protein motif:HMM:NF015684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HIR5165852.1"
                     /translation="MRTLDRYVLRQWAMVFVVVMLGFPLLVIVIDLADKFEKYMVRGL
                     TKGHVALAYVYGMPDTMFLVLPAAVLFATVFTVGTMGRHSEITAAKASGVSFHRLVRP
                     LFLAAVAACLAGVGLGELVPIGAARKAELLGEKAIRTGARSNFVYRADGGWVYAIRSL
                     QIDSGELLDLILERVGTGPAYPTIVVTARRARYGRTGGWTLQDGRVRYLLDPGVERAF
                     EFATLRVRTMRETPADLLAEPKTPEEMRYVELGRYIDALARSGSDTRKLAVDRALKLA
                     IPFTCIIIALFGAPLAITNPRSGAAWGVAVSLATTFLFLLLMQLSRAVGGGGLLPPTL
                     AAWFPNLLVGVGAVWLLRKAQT"
     gene            2505..>4472
                     /locus_tag="AC16JC_13975"
     CDS             2505..>4472
                     /locus_tag="AC16JC_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014561084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR5165853.1"
                     /translation="MKLPWKPCLLIATIALSGMSALRAQGVTTAAIAGVVTDSSGAPL
                     GGARVIAVHRPSGSEYAAATRADGRFTIPGMRVGGPYRVTVTIVGHRPEAQDGIQLTL
                     GGTADLRFVLAQVSVEVAPIVVTATTSPVFSAERTGAATTISTVQIAHLPTISRRVED
                     LLRLTPEYSPMSFGFSFAGQDNRFNNMTIDGSYFNNSFGLAGEPGDRTGVAPISLDAL
                     EQLQVNIAPFDVRQGNFVGANINMATKSGTNQFRGSAYFNSRNDQFVGTKAGPNTFNP
                     GTFKYHEIGVAVGGPIIKNRLFFFGSYEDDGQTLPGTSFRANTGSEPVTGNVTRVLAS
                     DLDALSSYLSTNFGYAPGPYQGYNFEIPSTRFLARMDFNLNDRNKLSVRYNLLNSSSD
                     ILLSNSASLGLGFRRSSLNSLNFANSNYAILENLRSIVGEWNSALGGRMSNNLIVGYN
                     SSDESRKVISPPWFPLVEILNANTNYTTFGFEPFTPDNKLTYHSYQLQDNFTVYMQKH
                     SLTFGASVEKYHSNNVFFPGAQSVYVYNSLADWYTDANDYLANPGRTVSPVTLRRFQV
                     RYSNIPGQTEPVQPLDVLYYGVYAQDEWRPIPNLTLTGGLRIDAPKFKNTAYDNPVAD
                     TMSFRDAGGATVHYNSGALPGVNLLFSPRLGF"
BASE COUNT          693 a         1542 c         1501 g          736 t
ORIGIN      
        1 tcgtggcgat gacgctgccg atggccgtgc tggtggccgt gctgcacacg atgagccggc
       61 tggtcgccga cagcgaggtc acggccatga cggcgaacgg catcagcgtc ggccgcatcg
      121 cgcggcccgt gctgctgtgg gccgcggtcg tggcgatcct cgcgttcctg tggaacgatc
      181 agctgctgcc gcgctcgaat caccgtctcc gaacgctgct cgtggacatc cagcgcaaga
      241 agccgtccct cgccttgaag gagcaggtga tcaacgaggt cgtcccgggc cagttcttcc
      301 tgcgggcggc acggatcgat ccggccacca accggctgcg tgacgtgacg atctacgatc
      361 tgggcgatgc cgaccgtcgc cggatcatca cggccgacag cgggcagatg gcttataccc
      421 ccgggcagac cgatttgtac ctgacgctga gggagggcga catccaggag gtgcgccgcg
      481 ggcaggacgc gcagttcaac cgcacgttct tccacacgaa cttcatccgg gtccgcaacg
      541 tatcgaacca gctcgtgcgc accgaggagg acacgtatcg cggcgaccgg gagatgaccg
      601 tgtgcgagat ggcgcaggtc gtgcggtcgg ccggcaacga cgcgcgcatc gcccaccggc
      661 agggagacgc caccgtgcgg cgcgacctgc ggcgactcgc cgggctcgcg cccgtccccg
      721 cacccgtcgc cgacgccccg gcggacaccg cgcccggtgt cttctgtcgg tggctgggcc
      781 ggctgacgag ctggctgctg ccggcgtcgg cgcaggccgc gcagaccccg tcccctgcgg
      841 tgtccgtccc cgtgactccg ccggcgccgc cggtcgtgtc ccaggtcgtc ctcgtcacgc
      901 ccggcgggat ctcagcggag caggacggcg cggagagcgc gcggcgccgc gccgcggtct
      961 acctggtgga gatccacaag aagctcgcga tcgccgccgc ctgtttcgtg ttcgccgtgc
     1021 tcggcgtccc ggtcgcgctg cgcttcccgc gcggcggcgc gggcctcgtg atcggtgtca
     1081 gtgtggtggt gttcgcggtc tattatgtcg ggctgatcgt cggcgaggat ctgggcgacc
     1141 ggctgatcgt cagcccgttc ctgtcgatgt gggtgccgaa cctcttgttc gggacgctcg
     1201 ggctcctggg catctgggtg atgcgccggg cgagccccac ggcccgcggc ggagactggt
     1261 ccgatctcaa ggacgcgatc gtcgggctgt tccggcgccg cgcacacgcc tgatgcgcac
     1321 gctcgaccgc tacgtgctgc gccagtgggc gatggtgttc gtcgtcgtga tgctcggctt
     1381 cccgctgctg gtgatcgtca tcgacctggc cgacaagttc gagaaataca tggtgcgcgg
     1441 gctgacgaag ggacacgtcg cgctggcgta cgtgtacggc atgccggaca ccatgttcct
     1501 ggtgctgccc gcggcggtgc tgttcgccac ggtgttcacc gtcgggacga tggggcggca
     1561 ctccgagatc acggcggcca aggcgtcggg ggtgtcgttc caccgcctgg tgcggccgct
     1621 gttcctcgcc gccgtggcgg cgtgcctggc gggcgtgggc ctgggggagc tggtgccgat
     1681 cggcgctgcc cggaaagcgg agctgctggg cgagaaggcg atccgcacgg gggcgcgcag
     1741 caacttcgtg taccgcgccg acggcggctg ggtgtacgcc atccgcagcc tccagatcga
     1801 ctcgggcgaa ctgctcgacc tgatcctcga gcgcgtggga acgggcccgg cgtatcccac
     1861 catcgtcgtg acggcgcggc gggcgcgcta cggccggacg ggcggctgga cgctgcagga
     1921 cggccgcgtc cggtacctgc tggaccccgg cgtcgagcgg gcgttcgagt tcgcgacact
     1981 gcgggtgcgc accatgcgcg agacgcccgc ggatctgctg gccgagccca agacacccga
     2041 ggagatgcgg tatgtggagc tggggcgcta catcgacgcg ctggcgcgct cggggtcgga
     2101 cacacgcaag ctggcggtgg accgcgcgct gaagctcgcg attcccttca cctgcatcat
     2161 catcgcgctg ttcggcgcgc cgctcgccat caccaacccg cgcagcggtg cggcgtgggg
     2221 tgtggcggtg agcctcgcca ccaccttcct gtttctcctg ctgatgcagc tctcgcgcgc
     2281 ggtcgggggc ggcggactgc tgccgccgac gctggccgcg tggtttccca acctgctggt
     2341 gggtgtcgga gcggtgtggc tgctgcgcaa ggcgcagacc taggaacgat caaaggcccg
     2401 cgacttgacg acgcccgcca cgggcggagt tttagggaag cctcggccag gcccgtcttg
     2461 aacgccgccc gctggatctc ggccacacca tcgatggggg gaacatgaag ctgccctgga
     2521 aaccttgtct gctcatcgcg acgatcgcgc tctcaggaat gtccgccctg cgtgcccagg
     2581 gggtcaccac cgccgcaatc gcgggagtcg tgaccgattc ctcgggcgcg ccgctcggcg
     2641 gggcgcgggt gatcgccgtg caccggccat cgggatcgga gtatgccgcg gcgacccggg
     2701 cggacggccg gttcacgatc cccggcatgc gcgtcggcgg gccgtatcgc gtcacggtga
     2761 ccatcgtggg ccaccgcccc gaggcgcagg acggcatcca gctcaccctg ggcggcaccg
     2821 ccgacctgcg gttcgtcctg gcgcaggtga gcgtcgaggt ggctcccatc gtcgtgaccg
     2881 cgaccaccag cccggtgttc agcgccgagc ggaccggagc cgccacgacg atttccaccg
     2941 tgcagatcgc gcacctcccg accatcagtc gccgggtcga agacctgctc cggctcacgc
     3001 ccgaatacag ccccatgtcg ttcgggtttt cgttcgccgg gcaggacaac cggttcaaca
     3061 acatgacgat cgacggttcg tacttcaaca actcgttcgg gcttgccgga gagcccggcg
     3121 accggaccgg cgtggcgccc atttccctgg acgcgctcga gcagctgcag gtgaacatcg
     3181 cgccattcga cgtgcgccag gggaacttcg tgggcgccaa catcaacatg gccaccaaga
     3241 gcggcaccaa ccagttccgc ggctcggcgt atttcaactc gcgcaacgac cagttcgtgg
     3301 gcacgaaggc gggcccgaac accttcaacc cgggcacgtt caagtatcac gagatcggcg
     3361 tcgcggtcgg cggtccgatc atcaagaacc gcctgttctt cttcggcagc tatgaggacg
     3421 acggccagac cttgccgggc acctcgttcc gcgccaacac cggctccgag cccgtcaccg
     3481 gcaacgtcac ccgggtcctc gcctcggatc tggacgcgtt gagcagctat ctcagtacga
     3541 atttcggcta cgccccgggt ccgtatcagg gatacaattt cgagatcccc tcgacgcggt
     3601 tcctcgcgcg catggacttc aacctgaacg accgcaacaa gctgagcgtg cgatacaacc
     3661 tgctgaattc gagctccgac atcctgctgt ccaactccgc ctccctgggg ctgggattcc
     3721 gccgcagcag cctgaattcc ctgaatttcg ccaactcgaa ctacgccatc ctggagaacc
     3781 tccgctccat cgtgggcgag tggaattcgg cgctgggcgg ccgcatgtcg aacaacctga
     3841 tcgtcgggta caattcgagc gacgagagcc gcaaggtcat cagcccgccc tggttcccgc
     3901 tggtggaaat cctcaacgcc aacacgaact acacgacgtt cgggttcgag ccgttcacgc
     3961 ccgacaacaa gctgacctac cacagctacc agctccagga caacttcacc gtctacatgc
     4021 agaagcactc gctcacgttc ggggcgagtg tggagaagta tcactccaac aacgtcttct
     4081 tcccgggcgc gcagagcgtc tacgtctaca actcgctggc cgactggtac accgacgcca
     4141 acgactatct ggcgaacccg ggccgcacgg tctcgccggt cacgctgcgc cggttccagg
     4201 tccggtattc gaacatcccc ggccagacgg agcccgtgca gccgctcgac gtgctgtact
     4261 acggcgtcta cgcacaggac gagtggcgcc cgatcccgaa cctgaccctg acgggcgggt
     4321 tgcggatcga cgccccgaaa ttcaagaaca ccgcctacga caacccggtg gccgacacga
     4381 tgtcgttccg cgatgccggc ggcgcgacgg tgcactacaa cagcggggcc ctgccggggg
     4441 tcaacctgct gttttcgccg cggctcggct tc
//
LOCUS       DBNGZW010000592         8678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1745503, whole genome shotgun sequence.
ACCESSION   DBNGZW010000592 DBNGZW010000000
VERSION     DBNGZW010000592.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1745503"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /gene="arsN2"
                     /locus_tag="AC16JC_13980"
     CDS             <1..511
                     /gene="arsN2"
                     /locus_tag="AC16JC_13980"
                     /inference="COORDINATES: protein motif:HMM:NF040501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arsenic resistance N-acetyltransferase ArsN2"
                     /protein_id="HIR5165854.1"
                     /translation="IVDFPTPLARALLRVGRPFPPPEPPMDVRIESARPEDLAAILAL
                     LDQHRLPHAAIERHLADAVVARDGAQVVGCAAVERYGASGLLRSVAVDGPHRGTGLGG
                     RLTAAALAHARAHGVRTVYLLTETAAAYFPRFGFRPIARDTVDPAVRASVEFTSACPE
                     SALAMAKEL"
     gene            515..1747
                     /locus_tag="AC16JC_13985"
     CDS             515..1747
                     /locus_tag="AC16JC_13985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5165855.1"
                     /translation="MQHRPYWGDAAPRPDLPVLKRLSTRTDAVVIGGGYTGISAARAL
                     AQAGAKVTVLERDVLGAGGATRNAGLVLSGFKRDARSLVKELGAVRARELYTASRAAV
                     TYLEEVLQAEGADAEFHRSGHLLLAAKPAHYVELEREAELLDKAFRHHTRLVPPEHIG
                     EELGATAYHGALLDPEAAAVNPGKLYWALAASAQRAGAELVEDVTVHEVHRSAGRFMV
                     RTSRGTLGAGHVVVATDGHADGLVRGLGRRVSPATGFVVATAPLGTSVARALIPRDRV
                     VSDARHLLVYFRITADTRMVFGGKPEIGKVKVGETIKHLVDTMCTLFPRLLGTDIDYH
                     WSGHIAITRDQMPHAGLRDGVHYAAGFNGQGLALAVYLGARLGRMVTTGKRGDLEPFL
                     RLPFPPIPLLHRLKTRLR"
     gene            complement(1827..2189)
                     /locus_tag="AC16JC_13990"
     CDS             complement(1827..2189)
                     /locus_tag="AC16JC_13990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165856.1"
                     /translation="MVRSVVAVFAGFLAIVVLVLASAPVIAKLLLPATTARPTRAYLL
                     ANLLTGFVFAGVGGWLAAHLAASGPHGHAVALAALVLALGIMTAAQGGAARAGQPVWY
                     GWVLPFVGAGGALLGGWI"
     gene            complement(2237..2785)
                     /locus_tag="AC16JC_13995"
     CDS             complement(2237..2785)
                     /locus_tag="AC16JC_13995"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR5165857.1"
                     /translation="MKVRVLVLAVSLSLLTRPVAAQKAGTIELGGFGRFTDYDNSLVF
                     TNKVGIGGRAGVFLPAHFSVEADYSSTSNDLPPAKVKYKPLHVMLLYNYPLAESSVFV
                     GGGYARNKYAYQTPSASSDTTDSGIAMVVGVRYRIQNLVALRLDLNEDFIPSPANKGP
                     NTSFNGNLGMQLGVSVLVSVLK"
     gene            complement(2878..3972)
                     /locus_tag="AC16JC_14000"
     CDS             complement(2878..3972)
                     /locus_tag="AC16JC_14000"
                     /inference="COORDINATES: protein motif:HMM:NF013705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HIR5165858.1"
                     /translation="MAQQRDYYRVLGVSETATTDEIKKAFRRLAKQYHPDRNPNNAQA
                     SDRFKEINEAHDVLSDADKRRKYDMLRKYGAFAGGGMSGGRTRGGYGTGGPSPQDLGP
                     DFDISDLGSFGGLGDLFSSIFGKRGKEEPEEAVETIVSIPFRVAALGGKLPIHLPMAE
                     VCPTCSGSGAAPGAKVSTCPECKGRGTISFGQGGFAVNRPCPVCRGKGKVPSERCPTC
                     RGSGEMSVEKRIMITVPPGTDDGTRMRLKGQGPKGKGDLVVQFQVEPDRFFRREGTDV
                     ISVVPINLAQALLGSRIKVRTLDGRHVVLRVPAGTQNGQKFRIAGHGIERNGKRGDQF
                     VEVKLELPENLTPEQVEAARQFAEKVGLKH"
     gene            complement(4001..4621)
                     /locus_tag="AC16JC_14005"
     CDS             complement(4001..4621)
                     /locus_tag="AC16JC_14005"
                     /inference="COORDINATES: protein motif:HMM:NF013212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR5165859.1"
                     /translation="MNKHRHKETPAEPAAAPQSPEEAPPAGGGALVEPAPEAVVRLED
                     ELGEARTALAQATDRYLRLAAEFENYKKRSVRERTEARARAQAELIERLVDGLDDLAR
                     FAHVDPAQTDAKTIHDGVDMVERQFWKQLDAVGVSRIDQVGVPFDPNVHEAVTMQPAA
                     QAAQDHTVGAVLQPGYRLGDALIRPARVVVLTWQGESGDGPAPSDG"
     gene            complement(4825..5373)
                     /locus_tag="AC16JC_14010"
     CDS             complement(4825..5373)
                     /locus_tag="AC16JC_14010"
                     /inference="COORDINATES: protein motif:HMM:NF024895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HIR5165860.1"
                     /translation="MKRYQLAGLVIALGLCAAPALHAQAQPTEGIRFGVGGGLTLPMG
                     NYADIDKAGWHLMGLIQLPISESPIHLRFDAMYGQTSHKSVFGSGSTTLTGATGDLLY
                     HLGDRAAKVRPYVLGGLGVYNVDLGGSSSSETKLAFGLGGGILFGVGTMHAFLEGRYM
                     SVQTSGSSLNFVPISLGVMFGR"
     gene            5629..6030
                     /locus_tag="AC16JC_14015"
     CDS             5629..6030
                     /locus_tag="AC16JC_14015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012843882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HIR5165861.1"
                     /translation="MNATTTSYAIGRSVPLAYEQAVARIKDALQQQGFGVLTEIDVQA
                     TFKKKLDVDFKKYVILGACNAPLAYQGFQAEPDIGLLLPCNVVVYEETPTRSRVALLD
                     PVAQLGISGREDLEPIARQAREKLERALQQV"
     gene            complement(6041..6877)
                     /locus_tag="AC16JC_14020"
     CDS             complement(6041..6877)
                     /locus_tag="AC16JC_14020"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HIR5165862.1"
                     /translation="MPELRAPDGRSLVYDVYEPAGDVAPRAAILWVCGWSDHRTRWRA
                     PAARLRDAGYAVYVLDQRGQGDSGGVRGHLSRFSQLLGDLQALRRMVRQRGDLPHVLL
                     GHSFGGLVVLRYLETQPSDPVAGAVLSAPWLATALPVALWKHLAAKVLADLWPTARFQ
                     TGLDSAALARDASVAAEYDADPAVHRIMTPGAWREIQWAQRVVPVDARRIECPLLFLL
                     GGADRIVDVGTTRTMADGLRNGTEAQVEVRWYPESYHELLHDREADTVSNDVLAFLGR
                     LW"
     gene            complement(6877..8151)
                     /locus_tag="AC16JC_14025"
     CDS             complement(6877..8151)
                     /locus_tag="AC16JC_14025"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR5165863.1"
                     /translation="MKLEVVTIGSELLLGFALDTNALDLGTALAAAGIEVTRRTSVAD
                     RPDAVRDAVRDALDRTGAVLTTGGLGPTRDDLTKQAVAGLFGRELVLYEDVLRHIEER
                     FRRFGRAMTPSNRIQAELPRGSVVLPNPRGTAPGIWLEDGARLVIMLPGVPVEMRGLL
                     VEEVLPRLVARARGSAGEAEPRVVRSRTVRTTGMAESAVGEKVGPLEEGLAPLTLAYL
                     PSIAGVDLRLTAWGLPPATADQRLAQAVERLRAELGERCYGEGSDDLAGVVLDLLRRR
                     GARLAVAESCTGGMLGQRITAIPGSSDVFVGGVIAYQDAVKTALLDVPRDTLERQGAV
                     SEETVRAMAEGAAHRFGVACALAITGIAGPAGGTPEKPVGTVWIAARHGGETRALRRQ
                     FLGERDEIRARSAQAALDLLRHALLETGSRRD"
     gene            complement(8148..>8678)
                     /locus_tag="AC16JC_14030"
     CDS             complement(8148..>8678)
                     /locus_tag="AC16JC_14030"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR5165864.1"
                     /translation="IFLAAAFSDVVDGYMARKYNQVSELGQLLDPIADKLMLFAMLVP
                     IFWITRHPTILVDYRIPWWGNLPVWVLLLLVGREVLITAFRFFAKRQGVVIPAAAAGK
                     LKAVVQNVFIGATILWFAWKDWLASHRFAGWFRNFWDQFHGAVVALTLGIAIVLTVYS
                     LAVYLYRYRRLITDRW"
BASE COUNT         1305 a         3095 c         3024 g         1254 t
ORIGIN      
        1 gattgtagat ttccccaccc ccctggcccg cgccttgctc cgggtcggtc ggccctttcc
       61 cccgccggag ccgccgatgg acgtccgcat cgaatccgcc cgccctgaag acctggcggc
      121 gattctcgcg ctcctcgacc agcatcgcct gccccacgcc gcgatcgagc ggcacctggc
      181 cgatgcggtc gtggcgcgcg acggggcgca ggtcgtgggc tgcgccgcgg tggagcgcta
      241 cggcgcgtcc ggcctgctgc gctcggtcgc cgtggacggt ccgcaccgcg gcaccgggtt
      301 gggcgggcgg ctcaccgccg ccgcgttggc gcacgcccgc gcgcacgggg tgcgcacggt
      361 ctacctgctc accgaaacgg ccgcggcgta cttcccccgc ttcgggttcc ggcccatcgc
      421 gcgcgacacg gtggaccccg cggtgcgggc gtcggtcgaa ttcacgagcg cctgtcccga
      481 gagcgccctg gcgatggcga aggagctgta acccgtgcag caccgtccct actgggggga
      541 cgcggcgccg cgccccgacc tccccgtgct gaagcggctc tccacgcgca ccgacgcggt
      601 ggtgatcggc ggcggctaca ccggcatctc ggccgcgcgc gcgctggcgc aggcgggcgc
      661 caaggtgacg gtgctcgagc gcgacgtgct cggcgcgggc ggcgccacgc gcaacgccgg
      721 attggtgctc tcgggcttca agcgcgatgc acgctcgctg gtgaaggagc tgggggcggt
      781 gcgcgcgcgg gagctgtaca cggcgtcgcg cgcggcggtg acctacctgg aagaggtgct
      841 gcaggccgag ggggcggacg cggaattcca ccggtcgggg cacctgctcc tcgccgccaa
      901 gccggcgcac tacgtggagc tcgagcgcga ggcggagctg ctcgacaagg cgttccgtca
      961 ccacacccgg ctcgtgcccc cggagcacat cggggaggag ctgggggcca cggcctacca
     1021 cggcgcgctg ctggacccgg aagccgccgc ggtgaatccc ggaaagctct actgggcgct
     1081 cgccgcctcg gcgcagcgcg caggcgcgga actggtggag gacgtgacgg tccacgaggt
     1141 gcaccgctcc gccggccgct tcatggtccg gacgtcgcgc ggcacgctgg gcgccggcca
     1201 tgtggtggtc gccaccgacg gccacgccga cggcctggtc cggggcctgg gccgccgcgt
     1261 ctcacccgcc accggcttcg tggtcgccac cgctccgctg ggcacgagcg tggcgcgcgc
     1321 gctgatcccg cgcgaccgcg tggtgagcga tgcgcgacac ctgctggtgt atttccgcat
     1381 caccgccgac acccgcatgg tgttcggcgg caagccggag atcggcaagg tgaaggtggg
     1441 ggagacgatc aagcacctgg tggacacgat gtgcaccctg tttccccggc tgctgggcac
     1501 cgacatcgac tatcactggt cgggccacat cgcgatcacg cgcgatcaga tgccgcacgc
     1561 cggcctgcgc gacggggtac actacgcggc gggattcaac ggacagggcc tggcgctcgc
     1621 cgtgtacctc ggcgcccggc tgggacggat ggtgacgacc gggaaacggg gcgacctcga
     1681 gccgtttctt cgcctcccgt tccccccgat ccctctcctc caccggctga agactcgact
     1741 caggtgaaac gggggaaggg ggaacaggga agggggaagg ggccgctacc cttccccctt
     1801 ccctcttccc ccttccccgt ctttgcctag atccagcccc ccagcaatgc cccccccgcc
     1861 cccacgaagg gcagtaccca cccgtaccag accggttgac cggcgcgcgc ggcgccgccc
     1921 tgagccgccg tcatgatgcc gagcgccagc accagcgccg ccagggcgac ggcgtgcccg
     1981 tggggacccg aggcggcgag gtgcgccgcg agccacccgc ccacgcccgc gaagacgaat
     2041 ccggtcagga gattggcgag caggtacgcg cgggtgggcc gcgctgtggt cgcgggcagg
     2101 aggagcttcg cgatgaccgg ggcactcgcc agcacgagga cgacgatcgc cagaaatccc
     2161 gcgaacaccg cgacgacgct gcgcaccatc tacggcgggc ctcctccggg cgcggacggc
     2221 ccgccgttcg atcccgtcat ttcaggacgc tgaccaggac gctcacgccc agctgcatgc
     2281 cgaggttgcc gttgaacgag gtgttcggtc ccttgttggc gggagacggg atgaagtcct
     2341 cgttcaggtc gagccgcagc gccaccaggt tctgaatgcg gtatcgcacg cccacgacca
     2401 tcgcgatgcc ggagtcggtc gtgtccgagg acgccgaagg cgtttgatag gcatacttgt
     2461 tgcgcgcgta gccaccgccc acgaagacgg acgactcggc cagcggatag ttgtacagca
     2521 gcatgacatg cagcggcttg tatttcacct tcgccggagg gaggtcgttg gacgtgctgg
     2581 agtagtccgc ctcgaccgag aagtgggccg ggaggaagac tccggcccgc ccgccgatcc
     2641 ccaccttgtt ggtgaacacc aggctgttgt cgtagtcggt gaatcgcccg aagcccccca
     2701 gctcgatggt ccccgccttc tgggcggcga cgggacgggt caggagcgac agggatacgg
     2761 cgagaaccag aacccggact ttcatgacgg cccccctctg gtggagaacg ggttgcgggc
     2821 ctgaatctgt gggtcatcgc ggcactcgtc cagcgccacc cctcccctcc gcactcctca
     2881 gtgtttcagg cccaccttct cggcgaactg ccgcgccgcc tcgacctgct cgggggtgag
     2941 gttctcgggc agctcgagct tcacctcgac gaactgatcg ccccgcttgc cgttgcgctc
     3001 gataccatgg cccgcgatgc ggaatttctg gccgttctgg gtcccggccg gaacgcgcag
     3061 cacgacgtgc cggccgtcca gggtccgtac cttgatgcgc gaccccagca gggcctgggc
     3121 gaggttgatg ggcaccacgg agatgacgtc ggtcccctcg cgccggaaga accggtcggg
     3181 ctccacctgg aactggacca cgagatcgcc cttgcccttg gggccctggc ccttgaggcg
     3241 catgcgcgtg ccgtcgtcgg tgccgggcgg cacggtgatc atgatgcgct tctcgaccga
     3301 catctccccc gacccgcggc aggtgggaca gcgctcgctc ggcaccttcc ccttgccgcg
     3361 gcacaccggg cagggacgat tcaccgcgaa gccgccctgg ccgaaggaga tcgtgccgcg
     3421 gcccttgcac tcgggacagg tggagacctt ggccccgggc gccgcgccgc tgccggagca
     3481 ggtcggacac acctcggcca tgggcaggtg gatggggagc ttgccgccca gtgccgcgac
     3541 gcggaacggg atcgagacga tggtctcgac cgcctcttcc ggctcctcct tgccccgctt
     3601 gccgaagatg gagctgaaca ggtcccccag cccgccgaag gagccgaggt cggagatgtc
     3661 gaagtcgggc cccagatcct gcggcgacgg gccgcccgtg ccatagccgc cgcgcgtgcg
     3721 gccgccgctc atgccgccgc ccgcgaacgc gccgtacttg cgcagcatgt cgtacttccg
     3781 gcgcttgtcg gcgtcgctca ggacgtcgtg cgcctcgttg atctccttga agcggtcgga
     3841 cgcctgggcg ttgttgggat tgcggtcggg atggtactgc ttggccagcc gccggaacgc
     3901 cttcttgatc tcgtccgtcg tcgcggtctc ggagacgccg aggaccctgt agtagtcgcg
     3961 ctgttgggcc atggggctgt cggctatcgg ctatcggctg tcagccatcg gacggagctg
     4021 gaccgtcgcc ggattcacct tgccacgtca gcacgacgac gcgcgcggga cgaatcaacg
     4081 cgtcgcccag ccggtaaccc ggctgcagca ccgcgcccac ggtgtgatcc tgggcggcct
     4141 gggccgccgg ttgcatggtc acggcctcgt gcacgttggg gtcgaacggg acgccgacct
     4201 gatcgatgcg gctgacgccg acggcgtcga gctgcttcca gaactgtcgc tcgaccatgt
     4261 ccacaccgtc gtggatcgtc ttggcgtcgg tctgcgccgg gtccacgtgg gcgaagcgcg
     4321 ccaggtcgtc gagcccgtcc accagccgct cgatcagctc ggcctgggcc cgcgcccgcg
     4381 cctcggtgcg ctcgcgcacg ctgcgcttct tgtagttctc gaactcggcc gcgagccgga
     4441 ggtagcggtc cgtggcctgg gccagcgcgg tgcgcgcctc gcccagctcg tcctccagcc
     4501 gcaccacggc ttcgggggcg ggctcgacca gcgccccgcc cccggcaggc ggcgcttcct
     4561 cagggctctg cggcgccgcc gccggctcgg ccggcgtctc cttgtgtctg tgcttgttca
     4621 ttcccacctg ctccgagcaa ccgatgtgcc gggctgacgc gccgttttgg cgggcccgag
     4681 cctggggaat actctggaac ttgctatcct gcaacaagat agccccaaac ccggtcgctg
     4741 tcgagcgggc cgcagaaaaa gtgaggcggg agtacgactg gccgcccggc ccggccgaag
     4801 accgggcggc cgtagagcca ctgcttatcg gccgaacatc acgcccagac tgatgggcac
     4861 gaagttgagc gatgagccgc tggtctggac gctcatgtag cgcccttcga ggaaggcgtg
     4921 catggtgccg acgccgaaca ggatgccccc gccgagcccg aaggccagct tggtttccga
     4981 gctcgacgag ccgccgagat cgacgttata gacgcccagg cctcccagga cgtagggccg
     5041 caccttggcc gcccggtcgc ccaggtggta cagcaaatcg cctgtcgcac ccgtcaacgt
     5101 ggtgctgccg ctgccgaaga cgctcttgtg cgaggtctgc ccgtacatgg cgtcgaagcg
     5161 gaggtggatc ggcgactccg agatgggcag ctggatcagg cccatgagat gccagccggc
     5221 cttgtcgatg tccgcgtagt tgcccatggg gagcgtgagg ccgcccccga cgccgaaccg
     5281 gatgccctcg gtcggttgag cctgcgcgtg cagcgccggc gccgcgcaca gccccagcgc
     5341 gatgacgaga ccggcaagtt gatagcgttt catgatttgc tccttgcgtg tcggtaaaaa
     5401 ggcaggcgga ccagtgcacg ccctactcct tgaagcacta tacccgggaa cccgaagcgg
     5461 gggacatcca tcctccgatt tgcggactcg ctccttcgca ctagaagcgc ctggtcccaa
     5521 gaacggcact tcgccctgga ttcatggatc cttcaccacc atcgcccatc atccgaggcg
     5581 tagcatcgac ggcgacacac ggctttcacg gtggaggcat tccgtctcat gaacgccacg
     5641 acgacatcct atgcgatcgg ccggtcggtc ccgctcgcct acgagcaggc agtcgcccgg
     5701 atcaaggacg cgctccaaca gcagggcttt ggtgtgctca cggagatcga cgtgcaggcc
     5761 acgttcaaga agaagctcga cgtggacttc aagaaatacg tgatcctggg cgcctgcaat
     5821 gcaccgctgg cgtatcaggg attccaggcc gagccggaca tcggcctgct gctaccctgc
     5881 aacgtggtcg tgtacgaaga gacgcccacg cgatcgcgcg tcgcgctgct cgaccccgta
     5941 gcacagctcg gcatcagcgg gcgcgaggac cttgaaccga tcgcacggca agcacgggag
     6001 aaactggagc gggcgctgca acaggtctga caattgtgcc tcaccacagc ctccccaaga
     6061 acgccagaac gtcgttactc accgtatccg cctcccggtc gtgcagcagc tcgtggtagg
     6121 actcgggata ccagcgcacc tccacctgcg cctcggtgcc gttgcgcagc ccgtcggcca
     6181 tggtgcgggt ggtccccacg tccacgatgc ggtcggctcc acctagaaga aaaagcagcg
     6241 ggcattcgat gcggcgcgcg tccaccggca cgacgcgctg ggcccactgg atttcccgcc
     6301 aggcccccgg ggtcatgatg cgatgcaccg ccggatcggc gtcgtactcc gccgcgacgc
     6361 tcgcgtcgcg cgcgagcgcc gccgaatcga gaccggtctg gaagcgcgcc gtgggccaga
     6421 gatcggcgag gaccttggcg gccaggtgct tccacagcgc caccggcaac gccgtggcga
     6481 gccagggcgc cgataacacc gcgcccgcca ccgggtcgga gggctgggtc tcgaggtagc
     6541 gcagcacgac gagcccgccg aaggaatggc ccagcagcac gtgcggcagg tcgccccgct
     6601 gccgcaccat gcgccggagc gcctgcaggt cgcccagcag ctggctgaag cgcgacaggt
     6661 ggccgcgcac gccgcccgag tcgccctggc cgcgctggtc gaggacgtac acggcgtagc
     6721 ccgcgtcgcg caaccgcgcg gcgggcgcgc gccagcgggt gcggtgatcg gaccagccgc
     6781 acacccagag gatcgccgcg cgcggcgcga cgtctcccgc cggctcgtac acgtcgtaga
     6841 cgagggagcg cccgtcgggg gcgcgcagct cgggcatcag tcccgccggg aaccggtctc
     6901 gagcagcgcg tggcgcagca gatcgagcgc cgcctgcgcg ctgcgggcgc gaatctcgtc
     6961 gcgctcgccc aggaattgcc gccgcagcgc gcgcgtctcg ccgccgtggc gggccgcgat
     7021 ccacacggtc ccgaccggct tctccggcgt gccccccgcg ggcccggcga tcccggtgat
     7081 cgccagcgcg cacgcgacgc cgaagcggtg cgccgcaccc tcggccatgg cccgcaccgt
     7141 ctcctccgac accgcgccct gccgctcgag cgtgtcgcgc ggcacgtcga gcagcgcggt
     7201 cttcacggcg tcctggtagg cgatcacgcc ccccacgaac acgtccgagg agccggggat
     7261 ggcggtgatg cgctggccca gcatcccgcc ggtgcaggac tcggccaccg ccagccgagc
     7321 cccgcgacgc cgcagcaggt cgagcacgac gccggccaga tcgtcgctgc cctcgccgta
     7381 gcagcgctcg cccagctcgg cgcgcagccg ctccaccgcc tgcgccagcc gctggtcggc
     7441 cgtcgccggc ggcaggcccc aggcggtgag ccgcaggtcc acaccggcga tcgaggggag
     7501 ataggcgagc gtgagcggcg ccagcccttc ttccagcggg ccgactttct cccctaccgc
     7561 cgattcggcc atcccggtgg tgcgcaccgt ccgggagcgg acgacgcgcg gctcggcctc
     7621 gccggcgctc ccccgggcgc gcgccaccag gcgcggcagc acctcctcga ccagcagtcc
     7681 ccgcatctcg accggcacgc cgggcagcat gatcacgaga cgcgcgccgt cctcgagcca
     7741 gatcccgggt gccgtgccgc gcgggttggg gagcaccacg gagccgcgcg gcagctccgc
     7801 ctggatgcgg ttggaggggg tcatggcgcg cccgaagcgg cggaagcgct cctcgatgtg
     7861 gcgcagcacg tcctcgtaca gcaccagctc ccgcccgaag agcccggcca cggcctgctt
     7921 ggtgaggtcg tcgcgggtgg gcccgagccc gcccgtggtg aggacggcgc cggtgcggtc
     7981 gagcgcgtcg cgcaccgcgt cgcgcacggc gtcgggccgg tcggcgacgc tggtgcggcg
     8041 cgtgacctcg atcccggcgg cggccagcgc ggtgccgaga tccagcgcgt tggtgtcgag
     8101 cgcgaagccc agcagcagct cgctgccgat ggtgacgact tcgagcttca ccagcggtcc
     8161 gtgatgagcc ggcggtagcg gtagaggtag acggcgagcg agtacacggt gagcacgatg
     8221 gcgatgccga gcgtgagggc gaccacggcg ccgtggaact ggtcccagaa gttgcggaac
     8281 cagccggcga agcggtggct cgcgagccag tccttccagg cgaaccagag gatggtggcg
     8341 ccgatgaaca cgttctgcac cacggccttg agcttgccgg cggcggcggc gggaatcacc
     8401 acgccctgcc gcttggcgaa gaagcggaac gcggtgatca gcacctcgcg ccccaccagc
     8461 agcagcagca cccacaccgg gaggttgccc caccagggga tgcggtagtc cacgagaatg
     8521 gtggggtggc gcgtgatcca gaagatggga acgagcatgg cgaacagcat cagcttgtcg
     8581 gcgatggggt cgagcagctg gcccagctcg ctcacctggt tgtacttgcg ggccatgtac
     8641 ccgtccacga cgtcggagaa ggcggcggcg aggaagat
//
LOCUS       DBNGZW010000593         1715 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1897437, whole genome shotgun sequence.
ACCESSION   DBNGZW010000593 DBNGZW010000000
VERSION     DBNGZW010000593.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1715)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1715)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1715
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1897437"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..236)
                     /locus_tag="AC16JC_14035"
     CDS             complement(<1..236)
                     /locus_tag="AC16JC_14035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5165865.1"
                     /translation="MRRLLLGLSVLPAFLAAQQATGSTLTQFLGYAYPSELVAAPHGA
                     AVAWVLNERGARNIWEAQAPDWTPRRLTGYTADDG"
     gene            326..>1715
                     /locus_tag="AC16JC_14040"
     CDS             326..>1715
                     /locus_tag="AC16JC_14040"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR5165866.1"
                     /translation="MARRSSVGPWLAAGVVQVLLVASARPAAAQDSARTDSTGRPAVT
                     RATLQAALDLMGLSYSDSEFALLLRPRGQFGNEFTGRGDRRNAYDSIRAVPLDNADVP
                     ALFFQPLPPAVPVAARRPRFTDRPKQIRRPANLEDVAFWPVTDLAELLRTRAVTSVEL
                     TTMYLGRLRRFDPQLHAVVTLTDSLALSQARAADREIAAGHYRGILHGIPYGVKDLYA
                     VPGYPTTWGAAPYRDQVINTTATTVRRLEQAGAVLVAKLTSGALAMGDVWYGGRTRNP
                     WNLAEGSSGSSAGPAAAVSAGLVPFALGTETLGSIVSPSTRTGVTGLRPSFGRVSRAG
                     VMALAWSMDKAGPLCRNAEDCAVVFDAIRGADPADPATVNVPFPYDARARLSGLRIGY
                     IKSAFDANHRGDTLDRAVLEVLRSQGAQLIPIELPKRPAQALRIILSAEAAASFDDLT
                     RSGRDSLLTEQGA"
BASE COUNT          248 a          638 c          576 g          253 t
ORIGIN      
        1 ccgtcgtccg ccgtgtaccc ggtcaggcga cgtggcgtcc agtccggggc ctgtgcctcc
       61 cagatgttgc gcgcgccgcg ctcgttcagc acccacgcga ccgcggcccc gtgcggcgcg
      121 gccacgagct ccgacggata cgcgtagccg aggaactgtg tcagggtcga cccggtggcc
      181 tgttgtgccg cgaggaaggc tggaagaacg ctgaggccca ggagcaaacg acgcatcaga
      241 cgactccggt gaggagaaat ggggcgagtg gcgagtcgaa cagatgacgg tagactactt
      301 gacccgcact ccacaaggtg actcgatggc tcgtcgatcg tccgttggtc cctggctcgc
      361 ggccggggtc gtccaggtgc tgctcgtcgc gtcggcgcgt cccgcggcgg cgcaggactc
      421 cgctcgcacc gattccactg gtcggcccgc ggtcacccgg gcgaccctgc aggccgcgct
      481 cgacctgatg ggcctcagct acagcgactc ggagtttgcc ctgctgctgc ggccgcgcgg
      541 ccagttcggc aacgaattca cggggcgcgg cgaccgacgc aacgcctacg actcgatccg
      601 cgctgtgccg ctcgacaacg ccgacgtgcc ggcgctcttc ttccagccgc tccccccggc
      661 ggtccccgtc gccgcccggc gcccgcgctt caccgaccgg cccaaacaga tcaggcggcc
      721 ggccaatctc gaggacgtcg cgttctggcc ggtcaccgat ctcgccgagc tgctgcgcac
      781 tcgcgctgtg acgtccgtcg agctcaccac gatgtacctg ggccggctgc gccgcttcga
      841 tccccagctg cacgccgtcg tcacgctgac cgacagtctc gccctttcgc aggcgagggc
      901 ggccgatcgc gaaatcgccg ccggtcacta tcgcgggatc ctgcacggca tcccctacgg
      961 agtaaaggac ctctacgcgg tgcccggtta ccccaccacc tggggggccg ctccctatcg
     1021 ggatcaggta atcaacacca cggcgacgac ggtgcggcgg ctcgaacagg caggcgccgt
     1081 gctcgtggcg aaactcacat cgggcgcgct cgcgatgggc gacgtctggt acggcggccg
     1141 gacccgcaac ccctggaacc tcgcggaagg atcgagcggc tcgtcggcgg gaccggccgc
     1201 tgccgtctcg gccggcctcg tgcccttcgc gctcgggacc gagacgctcg gatcgatcgt
     1261 gtcgccctcc acccgcacgg gcgtgacggg actgcgaccc agcttcggcc gggtgagccg
     1321 ggccggcgtg atggcgctgg cctggtccat ggacaaggcg ggccccctgt gtcgcaacgc
     1381 ggaggactgc gctgtggtgt tcgacgccat ccgcggcgcc gaccccgccg accctgcgac
     1441 cgtgaacgtc cccttcccgt acgatgcccg cgcccggctg tccggactgc gcatcgggta
     1501 catcaaatcg gccttcgacg ccaaccaccg cggtgacacc ctggaccgcg cggtgctgga
     1561 ggtcctgcga agccagggcg cgcagctgat ccccatcgag ctgcccaagc ggccggccca
     1621 ggcgttgcgc atcatcctct cggccgaagc ggcggcgagt ttcgacgatc tcacgcgctc
     1681 cgggcgcgac agcctgctca ccgagcaagg cgccg
//
LOCUS       DBNGZW010000594         6930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_666748, whole genome shotgun sequence.
ACCESSION   DBNGZW010000594 DBNGZW010000000
VERSION     DBNGZW010000594.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6930
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_666748"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..620)
                     /locus_tag="AC16JC_14045"
     CDS             complement(243..620)
                     /locus_tag="AC16JC_14045"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5165867.1"
                     /translation="MAHYKTLKAWQHGQRLAIECARAARNFPDYEQGALADRLRRACY
                     SVPLNIAEGNTRQGSRECRRFLDSARSSLAEVETIIEVARELEYVSPAEFGRLEALAN
                     ETGKTLYGLLRKISDATSRTPRT"
     gene            complement(705..2219)
                     /locus_tag="AC16JC_14050"
     CDS             complement(705..2219)
                     /locus_tag="AC16JC_14050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165868.1"
                     /translation="MKTARLGYLYLAVTACAVAVYLHALLNGYALDDVPIVAANPVVH
                     GWSALWRAFGEPYWPPSTGSALYRPLVLASYGLDWLIGGGGAAWFHLVNLAWHAGVSV
                     AVAGLAHRSTGSVRAALLAGTLFAVHPVHVEAVANVVGRAELMAALFAMVSVFLALEY
                     DRPGWSLAAWVPGLLSKETAVVVPVLVAAGWIIGVGRRPPARRMAAYGAGWMVLGSAY
                     LIARWSVLHSAPHQVLVAPVFVGASPLAVRLTAVSSFTDFVRLLVFPLRLRADYSPGE
                     RTLVTSLLDVRLTLGVLCFFAWLTLTIWAWRSGRRLEAFGLCWIAVALVPVANLVLPI
                     GVLVAERTLYLPSVGFALAVAARLKDVPARRLGAFAVPVLLAGGMRTALRVPVWRDTG
                     TVSRSVLRDSPRSYINPLLMAETYLTRRQADSALESIRASVRISDHFPKALLWGADIA
                     FQLRDPRLADSLLARLDRMCPRCLFYYEFEARVARSRGDSAVADSFLVRAAALR"
     gene            complement(2237..2857)
                     /locus_tag="AC16JC_14055"
     CDS             complement(2237..2857)
                     /locus_tag="AC16JC_14055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165869.1"
                     /translation="MSERSLMGDPPLVRDRSGSEPPEELGVDLLGRMDELQSENGRLR
                     RLGTMVLGAGALLLLGAAVLTIVAMRQISAAATVHARGFVLEDAAGQIRGAWGVTREG
                     GVQLVLADAQGKPRVRVSALEDGSPGLALVDANGHSRAALGLLSDGTITLVFADPEGH
                     SRTVLGLTASGASSLALADGQGVTRAGLGVAPNGKPTVTVDGPSSP"
     gene            complement(2909..4255)
                     /locus_tag="AC16JC_14060"
     CDS             complement(2909..4255)
                     /locus_tag="AC16JC_14060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase family protein"
                     /protein_id="HIR5165870.1"
                     /translation="MSATTIRVSDQVTDGAFPACPIAPETIADTGLSPEAITELLLKT
                     LYVQGARSGQELVAAMCLPFAIVDELLLTLQHQRMIEVKRTVGAGRGGYIFDLTNAGS
                     ERAREAMAAGQYVGPAPVPLEHYRTWIERQSIRNVHVTRERLTAAFADLVLDPAFVES
                     LGPAVNSAKSLFIFGDPGNGKTVMAEVMAGLLGGSFYLPYAVDVGGQIMVVYDPVYHR
                     PVAPDDLGGAAGEPQWLRDVPNHDRRYAHVARPVVMTGGELTLEQLDLQYDHDTKMYQ
                     APFQVKANGGVLILDDFGRQRVPPRELLNRWILPLEKRMDFLTLHTGSKFPVPFECLL
                     IFATNLDPGALVEEAFLRRINYKVQAQSPTRAQYEEIFRRCAAARDIPYRPEAVAQVY
                     RDFYEGRGIAPRGCNPRDLTDHVCDLARFLGVAPTLSSDLLDRACRSYFLDASEQG"
     gene            complement(4252..4623)
                     /locus_tag="AC16JC_14065"
     CDS             complement(4252..4623)
                     /locus_tag="AC16JC_14065"
                     /inference="COORDINATES: protein motif:HMM:NF014678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR5165871.1"
                     /translation="MSACIAVTNLTRRRCVGARVRLANRWWARLRGLLGRRPLAEGEG
                     LMLVPCRAVHMFGMRYPVDVVFVDSTGGVVATYHCLAPGSRSKWHRGAVAALEVPAGT
                     LATTDTQPGDSLIWYPNPVAA"
     gene            complement(4620..5549)
                     /locus_tag="AC16JC_14070"
     CDS             complement(4620..5549)
                     /locus_tag="AC16JC_14070"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR5165872.1"
                     /translation="MTYLAALLFGLAVSSVVWVVARLIPARPRSVTRQLAELERIGNQ
                     TLDSDRRQRQRHSRELKAVLELIGGRFGGDPRRRAQLRELLVHAGYREAGAAGVYWGA
                     RISLTVGLGFGLLVLVKALGGDMPRALIAAAWGAALGWVGPAVHVGGRARRRQKELQR
                     TLPDALDLMIVCVEAGLALNQALLRVAEEIRHVSRLTSEEFSLVNYEIRAGVPREEAL
                     RNLGERTGVADIRSLIAMLIQTDRFGTSIAQALRVHSDTLRTKRRQRAEEAAAKTTIK
                     LVFPLATCIFPALFVVILGPALVQIYRTLAGLR"
     gene            complement(5560..6525)
                     /locus_tag="AC16JC_14075"
     CDS             complement(5560..6525)
                     /locus_tag="AC16JC_14075"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR5165873.1"
                     /translation="MVGPALLVFASVALGTVAAALAIQWALEFRQRRAVVRQLRDFAA
                     AATAPSSSAALLPDAAVQAKWLRAIADRIPHVPGLGQVLEQGGLAWKPQSFMAASLGL
                     AMGAGLTMLLVFPVWPAVLLAAAGAGAVPYLYARRKRRRRLESFEEQLPEAIDLIGRA
                     MRAGHPLSAGLKMASEESPEPLAGEFRRVFEEQRFGMPFEEAILGIADRVGLIDVRIL
                     VTAILVQREVGGNLAEVLDKISYVIRQRFTIRRQLRVYTAQGRFSGIVLALLPVAVGS
                     ALFFINRDYMITLFFDPIARYFLVLAVILQILGYFWIRKIVDIEI"
     gene            complement(6519..>6930)
                     /locus_tag="AC16JC_14080"
     CDS             complement(6519..>6930)
                     /locus_tag="AC16JC_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011000609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HIR5165874.1"
                     /translation="HDGSLTTLHANSPRDALSRLETMISMASLNLPDKAMRQQIASAI
                     NVVIQVSRLSDGTRKLITISEIVGMEGDVITMQDIFTFERQGMDENGKVVGRFRATGL
                     RPRFTERLQAWGIELPAGLFSPVAERAAAESARW"
BASE COUNT         1225 a         2427 c         2294 g          984 t
ORIGIN      
        1 cggcagccaa ccgcaccgct tcctgcttga actcccgact aaactgccgg cgcttctctg
       61 gcatcggaca cctcctgcct ccaagatgga ggctcaggtc ggtgtccacc aaatcgggtc
      121 aacttcagtc cgggtcgagc gcccccgcgt ccaacgcgtc cgagccccca cccccggcgt
      181 cgcacggact tgtgcagagc ttccccaggg agccgccgag ccaccgagtg gacgctcagg
      241 ggttaggtgc gaggggtgcg cgaagtggcg tcgctgatct ttcgcaggag gccgtacagc
      301 gttttgccgg tttcgttcgc caaagcttcg agacggccga actcggctgg gctcacgtac
      361 tccagctccc tcgccacctc gatgatggtt tccacctccg cgagcgagct tcgagcgcta
      421 tcgaggaagc gacgacattc ccgcgagccc tggcgggtgt tgccctcggc gatgttcagc
      481 gggacgctgt aacaggcgcg gcggagtcga tcggccagcg cgccctgctc gtagtcgggg
      541 aagttgcgag ccgccctcgc gcattcgatg gcgagtcgct ggccatgctg ccaggccttc
      601 aaggtcttgt agtgtgccat gccggaaacg taggacgttg ctgcgacagg cagggcacca
      661 gaactacgcg ggtcgtgccg ttcctacgcc ggcgtccccg gcattcatct cagtgcggca
      721 gcgcggacga gaaacgaatc ggcaaccgct gagtctcccc ggctccgcgc cacccgagcc
      781 tcgaactcgt agtagaagag acaccgcggg cacatgcgat caagccgagc cagcagcgaa
      841 tccgccaggc ggggatcccg tagctggaag gcaatgtcgg ctccccacag gagtgccttc
      901 ggaaagtggt ccgagatccg cacggatgcc ctgatcgact cgagcgcgct gtccgcctgc
      961 cgccgcgtca ggtaggtctc ggccatcaac aagggattga tgtaggaccg aggcgagtcc
     1021 cgcaacacgc ttcgcgaaac ggtcccggtg tcgcgccata cgggaacccg cagcgccgtc
     1081 cgcatcccgc ccgccaacag caccggcacg gcgaaggctc ccagccgtcg tgccggcacg
     1141 tccttcagcc gggcggcgac agcgagggcg aaccccacgg aaggcaggta gagggtgcgc
     1201 tccgcgacca ggacccctat gggaaggacc aggttggcca cgggcaccag ggccacggca
     1261 atccagcaga gaccgaatgc ctcaagccgg cgaccgctac gccatgccca aatcgtgagc
     1321 gtcagccagg cgaagaaaca aaggactccc agcgtaaggc gcacatccag aagactcgtg
     1381 acgagggtcc gctcacccgg cgaatagtcc gcgcgaagcc tcaagggaaa aacgagcagt
     1441 ctgacgaaat cggtgaagga cgagaccgcc gtcaggcgta cggcgagcgg gctcgcgccg
     1501 acgaagacgg gcgccaccag cacctggtgc ggggccgagt ggagcacact ccagcgcgca
     1561 atcagatacg ccgatcctaa taccatccac cccgcaccgt acgccgccat ccggcgtgcc
     1621 ggcggccggc gaccgactcc gatgatccac ccggcggcga cgagaacggg gactacgacc
     1681 gccgtctcct tgctcaggag gccggggacc catgctgcca ggctccaccc gggacggtcg
     1741 tattccaggg cgagaaacac cgacaccatc gcaaagagcg cggccatgag ctcggctctc
     1801 ccgacgacgt tcgccacggc ttcgacgtgc acggggtgca cggcgaacag ggtgccggcg
     1861 aggagtgctg cgcgaacgga accggtcgat cgatgggcaa gccctgcgac ggcaacgctc
     1921 accccggcgt gccaagccaa attgacgagg tggaaccacg ctgcgccgcc gccgccgatt
     1981 agccaatcca gcccgtaaga cgcgaggacg agcggtcggt acagggcaga gccggtagac
     2041 ggaggccagt acggctctcc gaaggctcgc cacagggctg accagccgtg cacgaccgga
     2101 ttggccgcga cgatggggac gtcatcgagc gcgtacccgt tcaacagcgc gtggaggtag
     2161 acggcgaccg cacaggcggt caccgccaga tacagatacc cgagccgcgc ggtcttcaaa
     2221 ggcggcgggc ggccgctcag ggcgagctgg gcccgtcgac agtcactgtc ggcttgccgt
     2281 tgggcgccac gccgagcccg gcgcgcgtca caccctgccc atcggcgagc gcgagactcg
     2341 aggccccact ggcggtgaga ccgaggaccg tccggctgtg accctccggg tccgcgaata
     2401 cgagcgtgat cgtcccatcg gacaggaggc ccagggccgc acgcgaatga ccgttggcgt
     2461 ccaccagggc gagtcccggt gagccgtctt ccagagcgct cacccggacg cgaggcttcc
     2521 cctgggcgtc cgcgagcacg agctgcacgc cgccttcccg ggtcacgccc caggcgccac
     2581 ggatctggcc ggctgcgtcc tcgagcacga agccgcgtgc gtgtacggtc gccgccgccg
     2641 agatctgtcg catcgcgacg atcgtcagga cggccgcgcc gaggagcagg agcgctcccg
     2701 ccccgagcac catggtgccg agacggcgca ggcgaccgtt ctcactctgg agctcatcca
     2761 tgcggcccaa caggtccacg ccgagctcct ccggtggctc gctccccgac cgatctctca
     2821 ccagcggcgg atcccccatc agcgaccgtt cactcataaa gtcctctctc gttcaccagc
     2881 gaatatgggt agtccgcgcg ccgaggcttc atccctgctc ggaggcgtcc aggaaatagg
     2941 accggcaggc ccggtccagc aggtcactcg agagtgtcgg cgcgaccccc aggaagcgcg
     3001 cgaggtcgca tacgtgatcc gtcaggtccc gcgggttgca cccccgcgga gcgatgccgc
     3061 ggccctcgta gaaatcgcga tacacctgcg ccaccgcctc ggggcggtag gggatgtcgc
     3121 gtgccgcggc gcagcgccgg aaaatctcct cgtactgggc gcgggtcggg ctctgggcct
     3181 gcaccttata gttgatacgc cgcaggaatg cctcctctac gagcgctccc gggtcgagat
     3241 tagtcgcgaa gataaggaga cactcgaatg gaaccgggaa cttcgatccg gtgtgcagcg
     3301 tcaggaagtc catccgtttc tcgagcggca ggatccaccg attcaggagc tcgcgtggcg
     3361 ggacgcgctg ccgtccgaag tcgtccagga tcagcacccc gccgttcgct ttcacctgaa
     3421 agggcgcctg gtacatcttc gtgtcgtggt catactgcag gtccagctgc tccagcgtca
     3481 gctcgccgcc cgtcatcacg accggccggg ccacgtgggc gtatcggcga tcgtggttgg
     3541 gcacgtcccg cagccactgc ggctctccgg cggcgccgcc caggtcgtcc ggcgcgacgg
     3601 gtcggtggta cacggggtcg tacacgacca tgatctggcc gccgacgtcc accgcgtagg
     3661 gcaggtagaa ggaccccccg agcaggccgg ccatcacctc ggccatcacc gtcttgccgt
     3721 tccccgggtc gccgaaaatg aacagcgact tggcggagtt gactgcggga ccgaggctct
     3781 cgacgaacgc gggatccagc acgagatcgg cgaacgccgc cgtgaggcgc tcgcgcgtca
     3841 cgtgcacatt gcggatggat tgccgctcga tccaggtgcg atagtgctcg aggggaacgg
     3901 gcgccggtcc cacatactgg ccggcggcca tcgcctcccg ggcgcgctcg ctgcccgcat
     3961 tggtgaggtc gaagatgtac ccgccgcggc ccgccccaac cgtccgcttc acctcgatca
     4021 tccgctggtg ctgcagggtg aggagcagct cgtccacgat ggcgaagggc agacacatcg
     4081 cggcgaccaa ctcctgcccg ctcctcgcgc cctggacgta cagcgtcttg agtaggagct
     4141 cggtgatcgc ttcgggcgag agaccggtgt ccgcgatggt ctccggcgcg atggggcacg
     4201 cggggaatgc cccgtcggtg acctggtccg atacccgaat ggtcgtagcg ctcatgccgc
     4261 caccggattc gggtaccaga tcagggagtc gccgggctgc gtatccgtcg tcgccagcgt
     4321 tccggcaggt acctccaatg ccgcgacggc accccggtgc catttcgagc gggaccccgg
     4381 cgccagacag tggtaggtcg ccaccacgcc gcccgtacta tccacgaaga cgacgtcgac
     4441 cgggtaccgc attccgaaca tgtggaccgc tcggcacggg acgagcatca gcccttctcc
     4501 ctcggccagc ggacggcggc ccagcaggcc ccgcagccgc gcccaccagc ggttggcgag
     4561 gcgaacgcgc gccccgacgc agcgccgccg cgtgaggttg gtgactgcga tacaggcgct
     4621 catcgcaacc ccgcgagcgt gcggtaaatc tggaccagcg cgggaccgag gatcaccacg
     4681 aagagggcag ggaagatgca ggtggcgagg gggaacacga gcttgatcgt ggtcttcgcc
     4741 gccgcctcct cggcccgctg gcggcgcttg gtccgcaacg tatccgagtg cacccgcagc
     4801 gcctgggcga tcgaggtgcc gaatcggtcg gtctggatga gcatcgcgat cagcgagcgg
     4861 atatcggcaa cgccggtgcg ctcacccagg ttgcgcagcg cttcttcgcg cgggaccccg
     4921 gcgcggatct cgtagttcac gagcgagaac tcctcgctcg tgagacggct gacatgccgg
     4981 atttcctcgg ccacgcgcag cagtgcctgg ttcagggcca gtcccgcttc gacgcacacg
     5041 atcatcaggt ccagcgcgtc ggggagcgtg cgctgcagct ccttctgccg gcgccgggcc
     5101 ctcccaccga cgtgcacagc ggggcccacc caccccagtg ccgcacccca ggcggcggcg
     5161 atcagggccc gaggcatgtc gcctcccagc gccttcacca ggaccaggag tccgaacccc
     5221 agaccgacgg tgaggctgat ccgggctccc cagtacaccc ccgccgcgcc cgcctcacgg
     5281 tatccggcat gcaccaggag ctcgcgcagc tgggcccggc gacgcggatc gccgccgaag
     5341 cgcccgccga tgagctcgag caccgccttg agctcgcgcg aatggcgctg ccgctggcgg
     5401 cgatccgagt ctaatgtctg gttgccaatg cgctcgagct cggcgagctg gcgcgtcaca
     5461 ctgcgcggcc gggcgggaat cagccgggcg accacccaga cgacggacga caccgccagc
     5521 ccgaacagca gtgctgcaag gtaggtcacg ggaagcccct cagatctcga tgtccacgat
     5581 cttgcggatc cagaagtagc ccaggatctg caggatcacc gcaagcacca ggaagtagcg
     5641 ggcgatcggg tcgaaaaaca gcgtgatcat gtagtcgcga ttgatgaaga acagggccga
     5701 cccgacggcc accggcagca gcgccaggac gatcccgctg aagcgaccct gggcggtata
     5761 cacgcggagc tggcggcgaa tagtgaatcg ctggcggatc acgtacgaga tcttgtccag
     5821 cacctcggcg aggttgccgc cgacctcacg ttgcacgagg atcgccgtca ccaggatgcg
     5881 cacatcgatg agccccacgc gatccgcgat tcccaggatc gcttcttcga acggcatacc
     5941 gaagcgctgc tcttcgaaca cgcggcggaa ttcccccgcg agcggctccg gcgattcctc
     6001 gctcgccatc ttgagccccg ccgacaacgg atggcccgcc cgcatggcgc gtccgatcag
     6061 gtcgatcgcc tcggggagct gctcctcgaa cgactcgaga cgccggcgcc gcttgcgtcg
     6121 cgcatacaga tacggcaccg cgcccgcgcc ggcggcggcc agcaacaccg ccggccatac
     6181 cggaaagacg agcagcatcg tcaggccggc ccccatggcg aggccgagtg acgccgccat
     6241 gaagctctgg ggcttccagg ccagtccgcc ctgctccagc acctgaccga gaccaggcac
     6301 gtgagggata cggtcggcaa tcgcgcgcag ccacttggcc tggaccgccg cgtcgggcaa
     6361 cagcgccgcg gaggagctgg gggcggttgc ggcggcggcg aagtcgcgca gttggcgcac
     6421 caccgcccgg cgctgccgga attcgagcgc ccattggatc gccagcgccg cggcgaccgt
     6481 gccgagcgcg accgaagcga agaccagcag ggccgggcct accatcgggc gctctccgcg
     6541 gcggcgcgct ccgctaccgg cgagaacagg ccggcgggca gctcgatccc ccacgcctgg
     6601 agccgctccg tgaagcgcgg gcgcagaccc gtcgcacgga agcggcccac cactttcccg
     6661 ttctcgtcca tgccctgccg ctcgaaggtg aagatgtcct gcatcgtgat cacgtccccc
     6721 tccatcccca cgatctccga gatggtgatc agcttacggg tcccgtcgga gagccgggag
     6781 acctggatga cgacgttgat cgcgctcgcg atctgctggc gcatggcctt gtcggggaga
     6841 ttcagactcg ccatcgagat catcgtctcg agccggctca aggcgtcgcg cggagagttc
     6901 gcgtgcagcg tggtgagcga tccgtcgtgc
//
LOCUS       DBNGZW010000595         8537 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_110522, whole genome shotgun sequence.
ACCESSION   DBNGZW010000595 DBNGZW010000000
VERSION     DBNGZW010000595.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8537)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8537)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8537
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_110522"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1433)
                     /locus_tag="AC16JC_14085"
     CDS             complement(<1..1433)
                     /locus_tag="AC16JC_14085"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5165875.1"
                     /translation="MSDPRNATSSPSRFSGGAAASPTWGIVFWRELRDLWVGGKALTL
                     ILIYAVLLGIYSYMLAANAEVNLLPLREMILEMVKASIAVGLFICLIIAADSFSGERE
                     RGTLETHLLTPASRRQIAVGKFLAAVSPWPVALLFAVPYWAVLSKGDPVFGQALLWGT
                     VMGSILSPAVAGLGMLVSLWCSSNKTSMLVSLCLYLLLLLPTELSRPGRVETPAEVRR
                     AMLYALVNPWDGTSNFLSKIFINRLSPAEVWLLPALSALFAVIVLVLLFGFASGRLRL
                     DPAMGSGVRAFWQRWSGAARPPARRPSPARREPAVAARVPEVALPVGHLSRLVAARSV
                     PAGPVTPTWWVVFKKELGDLWIGGRALQLTVAYTIVLGIYTYITARDSTLSLVPPKEM
                     VYELTKAAMVACVFVGLIIGADSLSGERERATLEGLLLTPTSRRQIVIGKFLAAASPG
                     PAALAITIPYMRVLSQGDEVFAQAVLWG"
     gene            complement(1450..2307)
                     /locus_tag="AC16JC_14090"
     CDS             complement(1450..2307)
                     /locus_tag="AC16JC_14090"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5165876.1"
                     /translation="MSGERALRSGPGLRAWWVVCRRDLQEHWIGGRALMLLLLYTVVI
                     GVVTYLMVSSSQLDLTPPKEMVWSTLQTCMYVGVFMGLLIGADTLSGARDRAALETLL
                     LTPASRTQIILGKFLASMSPWPAAMAVTFPCLLVVAQGDAILYTALEWVGFVGTLLVI
                     GFTGLGMLVSFWSNSNRTSLFTSLTIYLLFLLPALLPGQAQKGLVGKFFQRSNPLAGA
                     DEFLEKVIVNNRSLHDYGSWLKGPIVFPIIVIILLFLYAGPRLRLQAGGASLFRLPWN
                     RAARLSPSR"
     gene            complement(2304..3128)
                     /locus_tag="AC16JC_14095"
     CDS             complement(2304..3128)
                     /locus_tag="AC16JC_14095"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5165877.1"
                     /translation="MSGHAIPVWRIVFGRELRDLWVGGKALSMLLIYTVLLGIYAYLF
                     ASNSELQLLPLNQMVLEIVKAAIFVGLFICLIIGADTVSGERERATLEGLLLTPASRR
                     EIVAGKFLAALSLWPVALAVAIPYWVVLSKGGPVLGQALRWGPLMGTLLAPAFAGLGM
                     LVSVWCNSNKTSMLVSVALYLVLILPAEYSGLATVTVSAQATERSNVFQLANPVGASD
                     RLLHDVIMGNAPAHQVWLWVTTPVLFLLIVLAVLFLYAGPGLRLEPGMKRLRPRPV"
     gene            complement(3125..4105)
                     /locus_tag="AC16JC_14100"
     CDS             complement(3125..4105)
                     /locus_tag="AC16JC_14100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5165878.1"
                     /translation="MTQSDTSPPRSEVLVAEGLQKNYGPRHALRGLTFTLRAGRLLGF
                     LGPNGAGKTTSIRILTTIMEPDAGRFTVDGISNEYPQQIRRRIGVLPESFGLPKQMTG
                     IEYLTYFGQLYGRTKSDARAIGLSLLEEVGLKDRVRSLVGTYSHGMRQRIGIARALVN
                     DPVVVFLDEPTIGLDPRGQQELLGLVKQIAHERSAGVILCSHLLSEVESVCDDVVIMS
                     SGEMVASGTVSDVIGRTEQNVVRIRVPAADIADSQRELEGVSGVRKVTAAGGMPGWLR
                     VEFEESADDRHINNRILDALLRADIPVLSLEPEGGRLQDVFLQLTAGGIR"
     gene            complement(4130..7267)
                     /locus_tag="AC16JC_14105"
     CDS             complement(4130..7267)
                     /locus_tag="AC16JC_14105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003801311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5165879.1"
                     /translation="MRSIVKSSLKFRYIVLALAIAMIGYGIAQIPTMPVDAFPEFAPP
                     RVEIQTPSLGMTADEVEALVTVPLEQAFNGVDNLDVMRSKSIPDLSSIELLFKPGTDV
                     VAARQLVQERLTTVLTTLPTWAAPPVIIPPVSSTGRVMKIGVSSKELSVMDLSMIAYW
                     TIRARLLGVPGVANVAIWGERIKMPQVQVDPQRMLAHRVSLNQVMEVTADALDVGILY
                     FSSGAVIGTGGYVQTPNQRLDIRHVLAISSPEDLGQVPIADRRRPDGTALRLSDVSNV
                     VWDTWPLFGDAVVNDGPGLMLVVEKFPWANTLDVTHGVEQALQELRPGLPGVEMDPNI
                     FRPADFIQVAVDNLTRALIIGCILVMGVIAAFLFQWRTALISMVAIPLSLMAGALVLY
                     LSGAVINTMILAGFVIAIGVVVDDAIIDVENIMRRLRLARQEGSTQSTASVILEASLE
                     VRQAIVHATLIDAVVVLPVFFIGGLSGAFFQPMALAYGLAVLASMLVALTVTPAMCLL
                     LLAKAPIVHQRQPLVVWLQGVYTKVLTRIVRRPQPVFVGTAVIMVIGVAVLPLLGESL
                     FPHFKERDFLIHWITKPGSSVVEERRIVTRLSKELRAIPGVRNFGSHIGQALLADELN
                     GVNFGENWISIDPDADYEETIAAIEGVVYGYPGLFHNVETYLNERIDEVLSGASQTFV
                     VRIYGPDLKIIHEKAEEVEDSLRGIPGLIDLNVQLQSQVPQIQIEVNLAAAQRYGLKP
                     GDVRRASATLLAGEEVGDIFRDGKAYDVNVWSTPATRQSVSSVRNLLIDTPNGGHVRM
                     GDVADVRIAPAQSVIYRENDSRRIDVGAEVTAGDLGAVMDAVDDQLETVQFPLGYHAV
                     ILGEFAEREAASTRLSIFAIAAALGVFLILLASFENTRLAILGFLTLPSALVGGLLAV
                     FFTGDVVSLGSLVGFLTVFGIAARNMIMLISHYQHLERQEGEVFGPTLALRGARERLS
                     PILMTALATGLALVPLVATGDIPGHEIEYPMAIVILGGLITSTLLNLFVVPSLYLRFG
                     KSKLSDAAAPIPVNA"
     gene            complement(7293..7742)
                     /locus_tag="AC16JC_14110"
     CDS             complement(7293..7742)
                     /locus_tag="AC16JC_14110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165880.1"
                     /translation="MKSNDRWIAVGLCIACLPLAACAKPPVEEEVESRAVKVEQVEGS
                     DLSRVTVTAEAAKRLDIQTAPLRTVMIRGTLRKVIPYAALLYDKDGNTWAYVATEDSL
                     TFVRASVTVDYIDGETAVLSEGPAVGTVVVTTGASELYGSEEEFEEE"
     gene            complement(7768..>8537)
                     /locus_tag="AC16JC_14115"
     CDS             complement(7768..>8537)
                     /locus_tag="AC16JC_14115"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5165881.1"
                     /translation="VRLGDVAHVRVVSSPTVIRHEAVKRYMDVMAGVQGRDHGAVAAD
                     VRARLLQVPLPLEYHAEVVGEYADRQASRARLLVVAIAALVGIFFLLQAALGSWRLAG
                     LAFAGLAAALVGGIAAVFITGGVLSLGSLAGLLAVFGLAARHGIMLMRHYQGLERHGG
                     ESFGSGLVLRGSSERVVAMVTATVAVVVALLPALFLGDVPGLEIVRPMAVAVLGGVFT
                     STFVNLLIVPALYLRLGVSGVRELELDMPGGLMEAPA"
BASE COUNT         1531 a         2931 c         2807 g         1268 t
ORIGIN      
        1 ccccacagga ccgcctgagc gaacacctcg tcgccctgag acagcactct catgtaggga
       61 atcgtaatgg ccagcgccgc gggcccgggg gaagcggccg cgaggaactt gccgatcacg
      121 atctgccggc gactcgtggg ggtcagcaac agaccctcga gggtggcacg ctcccgttcg
      181 ccgctgaggc tgtctgcgcc gatgatcaga cctacgaaca cgcaggccac catcgccgct
      241 ttggtgagct cgtagaccat ctccttgggg gggacaaggc tcagcgtgct gtcacgcgcc
      301 gtgatgtagg tatagatccc gagcacgatg gtgtaggcca cggtgagctg cagcgccctc
      361 cctccgatcc agaggtcgcc gagctccttc ttgaacacga cccaccaggt gggcgtcacc
      421 ggacctgccg gcacggaccg cgcggcgacg aggcgcgaca ggtggccgac cggaagcgcc
      481 acttcgggca cgcgggctgc gacagcgggc tcgcggcgag cggggctcgg gcgtcgggcc
      541 ggtgggcggg cggcgccgct ccagcgctgc cagaacgcgc ggactccgct gcccatggcg
      601 ggatccaggc ggaggcggcc gctcgcgaac ccgaataaca ggacgaggac gatgacggcg
      661 aacagggccg aaagcgccgg cagcagccac acctcggccg gcgacaatcg gttgatgaag
      721 atcttggaga ggaagttcga cgtaccgtcc cacgggttca ccagcgcgta cagcatggcg
      781 cgtcgcacct cggccggggt ctcgaccctc cccggccgcg acaactccgt cggcagcaac
      841 agcaggaggt agagacacag gctgaccaac atgctcgtct tgttcgagct gcaccacagg
      901 ctgacgagca tgcccaggcc ggccaccgcc gggctgagga tgcttcccat cacggttccc
      961 cacagcagcg cctgcccgaa gaccgggtct cctttggaca gtacggccca gtaggggacc
     1021 gcgaacagga gtgcgacagg ccagggggat acggcggcga ggaacttgcc gaccgcgatc
     1081 tgccggcgac tcgccggcgt cagcaggtgc gtttcgaggg tcccgcgctc ccgttcgccg
     1141 ctgaagctgt ctgcggcgat gatcaggcag atgaagagcc cgacggcgat ggacgccttg
     1201 accatctcca ggatcatctc ccgcagcggc agcaggttca cttccgcgtt cgcggcgagc
     1261 atgtacgaat agatgcccag caggaccgcg tagatgagga tcaaggtgag cgctttgccg
     1321 ccgacccaga gatcgcgcag ctcgcgccag aacacgattc cccaggtggg cgacgccgcc
     1381 gcaccccctg agaaccgaga tgggctcgag gtcgcgttgc gcgggtcgct catgtcaggt
     1441 cctccaggct taccgagacg gggacagccg cgccgcgcgg ttccagggca gccggaagag
     1501 gctcgccccg ccggcctgca ggcgaagccg tgggccggcg tacaggaaca gcaggatgat
     1561 cacgatgatc ggaaacacga tgggcccttt cagccacgat ccgtagtcgt gcagactgcg
     1621 attgttgacg atgacttttt ccaggaactc gtccgctccg gccaggggat tcgagcgttg
     1681 aaagaacttc cccaccagtc ccttctgcgc ctgcccgggc agcagcgccg gcagcaggaa
     1741 caggaggtag atggtgagac tggtgaacag gctggtgcgg ttggaattgg accagaagct
     1801 cacgagcatc cccagcccgg tgaatccgat caccagcagg gtgccgacaa agccgaccca
     1861 ctcgagtgcc gtatacagga tggcgtctcc ctgagccacc acgagcaggc agggaaacgt
     1921 gacggccatg gcggcgggcc acggcgacat gctcgcgagg aacttgccga ggatgatctg
     1981 cgtccggctg gcgggcgtca gcagcagcgt ttccagcgcc gcccgatccc gggcgccgct
     2041 gagcgtatcc gccccgatga gcaggcccat gaacacgccg acatacatgc aggtctgcag
     2101 cgtcgaccag accatctcct tgggcggcgt cagatcgagt tggctgctgc tgaccatgag
     2161 ataggtcacc acgccgatca ccaccgtata caggagcagc agcatcaggg cgcgaccccc
     2221 gatccagtgc tcctgcaggt cccggcggca gacgacccac caggccctca gccccggccc
     2281 ggaccgtagc gcgcgctcgc cgctcatacc ggcctcggcc gcagccgctt catcccgggc
     2341 tccaaacgca gacccgggcc ggcgtagagg aagaggacgg cgagcacgat gaggaggaac
     2401 aagaccgggg tggtcaccca gagccacacc tggtgtgccg gcgcgttgcc catgatgacg
     2461 tcgtgcagca gccgatcgct cgcccccacc ggattggcca gctggaagac attggagcgt
     2521 tccgtcgcct gcgcgctgac cgtcacggtc gccagtcccg aatactccgc cgggaggatc
     2581 agcacgaggt acagggccac gctgacgagc atgctggtct tgttggaatt gcaccacacg
     2641 ctcacgagca tgccgaggcc cgcgaacgcg ggcgcgagga gcgttcccat cagcggcccc
     2701 cagcgcagcg cctgccccag caccggcccg cccttggaga gcacgaccca gtagggaatg
     2761 gcgacggcca gggcgacggg ccagagggag agggccgcaa ggaactttcc cgccacgatc
     2821 tcgcgccggc tggccggcgt gaggagcaac ccctcgagcg tggcgcgctc gcgctcgccg
     2881 gacaccgtgt cggcgccgat gatcaggcag atgaacagtc cgacgaagat cgccgccttg
     2941 acgatctcca gcaccatctg gttcaacggc agcagctgca gctccgagtt gctggcgaac
     3001 agataggcgt aaatgcccag caggaccgta tagatcagga gcatggacag cgccttgcct
     3061 ccgacccaca ggtcgcgcag ctcccgtccg aacacgatcc gccacacggg gatggcgtgt
     3121 ccgctcaccg gattccaccg gcggtgagct gcaggaacac gtcctgcaac cgcccgcctt
     3181 cgggctcgag actgagcacc gggatgtcgg cgcgcagcaa tgcgtcgagg atccgattgt
     3241 tgatatgccg gtcgtcggcg gattcctcga actcgacccg cagccacccc ggcatcccac
     3301 cggcggccgt gaccttccgg acgccggaca cgccttccag ctcccgctgc gagtccgcga
     3361 tgtcggcggc gggcacccgg atgcggacca cgttctgctc ggtgcggccg atgacgtcgc
     3421 tcaccgtgcc gctcgcgacc atctcccccg agctcatgat gacaacgtcg tcgcacacgc
     3481 tttcgacttc ggacagcagg tgactgcaca ggatcacccc ggcgctgcgc tcgtgcgcga
     3541 tctgcttgac cagccccagc agctcctgct ggccccgcgg atcgaggccg atcgtcggct
     3601 cgtcgaggaa cacgaccacc ggatcgttga cgagcgcgcg cgcgatcccg atccgttgcc
     3661 gcatgccgtg gctgtaggta ccgaccaggg accggacgcg gtctttcagt ccgacctctt
     3721 cgagcagtga cagaccgata gcccgtgcgt cactcttggt gcgtccgtag agctgcccga
     3781 agtaggtgag gtattcgatc cccgtcatct gcttgggcag cccgaagctc tcgggcagca
     3841 cgccgatgcg gcgccggatc tgctgcggat actcgttgct gatgccgtcc accgtgaacc
     3901 gcccggcatc gggttccatg atggtcgtga ggatacggat cgaggtcgtc ttgccggcgc
     3961 cgttcggacc gaggaagccg agcaggcgcc cggcccgcag ggtgaacgtc aacccccgca
     4021 acgcgtgtcg cggcccgtag ttcttctgca ggccttcggc caccaggacc tcggaccggg
     4081 ggggagaggt atcgctctgt gtcatcggaa ctcggctcct tccttcgggt caggcgttta
     4141 ccgggatcgg ggctgcggcg tccgacagct tgctcttccc gaaccgcaga tagaggctgg
     4201 gcacgacgaa caggttgagc aacgtcgagg tgatgagacc gccgagaatc acgatcgcca
     4261 tcgggtactc gatctcgtga ccggggatgt cgccggtcgc gaccagcggg acgagggcga
     4321 ggccggtggc gagcgcggtc atcaggatcg gtgagagccg ctcgcgcgcc ccgcgcaggg
     4381 cgagggtcgg gccgaacacc tcaccttcct gccgctccag atgctggtag tggctgatca
     4441 gcatgatcat gttgcgcgcc gcgatcccga acacggtgag gaagccgacc agggacccga
     4501 gcgagacgac gtcgcccgtg aagaacaccg ccaggaggcc gcccaccagc gccgacggca
     4561 gcgtcaggaa cccgaggatc gcgaggcgcg tgttctcgaa cgaggccagg aggatcagga
     4621 acacgccgag cgccgcggct atcgcgaaga tcgacaggcg ggtcgaggcc gcttcccgct
     4681 cggcgaactc accgaggatc accgcgtggt accccagggg gaactgcacc gtctccagct
     4741 ggtcgtcgac ggcatccatc acggcgccga ggtcgcccgc ggtcacttcg gcgccgacgt
     4801 cgatgcggcg cgagtcgttc tcccggtaga tcacgctttg ggccggtgcg atccgcacgt
     4861 ccgcgacgtc tcccatccgc acgtgcccgc cattgggcgt gtcgatcagc agattgcgca
     4921 cgctggacac actctgccgc gtggcggggg tgctccacac gttgacgtcg tacgccttgc
     4981 cgtcgcggaa gatgtcgccg acttcctcgc cggccagcag cgtcgcggag gcccggcgca
     5041 cgtcgccggg cttcaggcca tagcgctgcg ccgccgccag attcacctcg atctggatct
     5101 gcggcacctg cgattggagc tgcacgttca ggtcgatgag cccggggatc ccgcgcagcg
     5161 agtcctccac ctcttcggcc ttctcgtgga tgatcttcag gtccgggccg tagatgcgca
     5221 ccacgaacgt ctggcttgcg cccgacagca cctcatcgat gcgctcgttc aggtacgtct
     5281 cgacattgtg aaacaggccg gggtagccgt agaccactcc ctcgatggcg gcgatcgtct
     5341 cttcgtaatc cgcgtccgga tcgatgctga tccagttctc gccgaagttg acgccgttga
     5401 gctcgtcggc cagcagcgcc tggccgatgt gcgagccgaa gttccggacg cccggaatcg
     5461 cgcgcagctc cttgctgagc cgggtgacga tgcgccgctc ttccaccacc gagctgccgg
     5521 gcttggtgat ccagtggatc aggaaatccc gctccttgaa gtgcgggaac agggattcgc
     5581 cgagcagcgg cagcaccgcg accccgatga ccatgatgac ggccgtcccg acgaataccg
     5641 gctgcggccg tcggacgatg cgcgtcagga ccttcgtata gacgccctgg agccagacca
     5701 cgagcggctg gcgctggtgc acgatgggcg ccttcgccag cagcagcagg cacatcgcgg
     5761 gggtgaccgt cagcgccacc agcatggagg ccagcaccgc caggccatag gccagcgcca
     5821 tgggctggaa gaacgcgccc gacagccccc cgatgaagaa gaccggcaac accacgacgg
     5881 cgtcgatcag cgtggcgtgg acgatggcct gccgcacttc cagggaggct tcaaggatca
     5941 cgctcgccgt cgattgggtg ctcccttcct gacgtgccag tcgcagtcgc cgcatgatgt
     6001 tctcgacgtc gatgatcgcg tcgtccacga cgaccccgat ggcgatcacg aaccccgcca
     6061 gaatcatcgt gttgatgacc gccccggaca ggtagagcac cagcgcgccg gccatcagcg
     6121 acagggggat cgccaccatg ctgatcaggg cggtgcgcca ctggaacagg aacgcggcga
     6181 tgacgcccat cacgagaatg cagccgatga tcagggcgcg ggtgaggttg tccaccgcca
     6241 cctggatgaa gtcggccggc cggaagatgt tcggatccat ctccactccg ggcaggccgg
     6301 gacgcagctc ctggagcgcc tgctccacgc cgtgggtcac gtcgagcgtg ttggcccagg
     6361 ggaacttctc cactaccagc atgaggccgg ggccgtcgtt gaccaccgcg tccccgaaca
     6421 ggggccaggt gtcccacacg acgttggaca cgtcgctcag gcgcagggcc gtgccgtccg
     6481 gccggcgacg gtccgcgatg gggacctggc ccagatcttc cggcgaggag atggccagga
     6541 catgccggat gtcgagtcgc tggttggggg tctggacgta cccgccggtg ccgatcaccg
     6601 ctcccgagga gaaatagagg atccccacgt cgagggcgtc ggcggtcact tccatgacct
     6661 gattgagcga gacccggtgt gccagcatgc gctgggggtc gacctgcacc tgcggcatct
     6721 tgatgcgctc accccagatg gccacgttcg ccacccccgg cacacccagc agacgcgccc
     6781 ggatcgtcca gtaggcgatc atggagaggt ccatcacgga cagctccttc gaggacaccc
     6841 cgatcttcat gacccgcccg gtggacgaca cgggcgggat gattaccggc ggtgccgccc
     6901 acgtcggcag cgtcgtgagg acggtggtga ggcgctcctg gaccagctgg cgtgccgcaa
     6961 cgacgtcggt cccgggcttg aagagcagct cgatcgacga gaggtccggt atcgatttgg
     7021 accgcatgac gtcgaggttg tccaccccgt tgaacgcctg ctcgagcggc acggtgacca
     7081 gggcttccac ttcatcggcc gtcatgccga gggacggcgt ctggatctcc acccggggcg
     7141 gcgcgaattc cgggaacgcg tccaccggca tggtggggat ctgggcgata ccatacccga
     7201 tcatcgcgat ggcgagcgcc agcacgatgt accggaactt caggctcgat ttgacgattg
     7261 agcgcatcac ggccggaaac ctccccgtcg ggtcattctt cctcgaactc ttcctcgctg
     7321 ccgtacaact cggatgcgcc ggtcgtgacg accaccgtgc ccacggccgg gccctccgac
     7381 aacaccgcgg tctcaccgtc gatgtagtcc accgtcacgg acgcccgcac gaacgtgagc
     7441 gagtcctcgg tcgcgacgta cgcccaggtg ttgccgtcct tgtcgtagag gagcgccgcg
     7501 taaggaatca ccttgcgcag cgtcccgcga atcatgacgg tccgcaacgg cgcggtctgg
     7561 atgtcgagcc gtttggccgc ctccgccgtc acggtcaccc ggctgagatc cgacccttcg
     7621 acctgctcga ccttcaccgc tcggctttcg acttcctctt cgaccggagg cttcgcgcag
     7681 gccgccaggg gcagacaggc gatgcacagt ccgacagcaa tccatcgatc gttggacttc
     7741 accgtttcct cctcaggttc cgccggatca ggcgggggct tccatcagac cgccgggcat
     7801 gtcgagctcg agctcgcgca cgccgctcac gccgagacgc aggtacagcg cgggcacgat
     7861 caacaggttg acgaacgtcg aggtgaacac accgcccagg acggcaaccg ccatcgggcg
     7921 cacgatctcg agaccgggga cgtcgcccag gaacaaggcg ggcagcagcg ccaccacgac
     7981 ggcgaccgtc gcggtcacca tcgcgaccac ccgttcggag gacccgcgga gtacgagtcc
     8041 cgagccgaac gattcgccgc cgtgccgctc gaggccctgg taatgccgca tcagcatgat
     8101 gccatggcgg gcggccaacc cgaaaaccgc cagcaggccc gccagcgacc cgagcgacag
     8161 caccccgccc gtgatgaaga ccgccgcaat tccacccacc agcgccgcgg caaggccggc
     8221 gaacgccaaa ccggccagcc gccagctccc caaggcggcc tggagcagga agaagatccc
     8281 gaccagcgcg gcgatggcga cgacgagcag gcgggcccgg ctcgcctgcc ggtcggcgta
     8341 ctcgccgacc acttcggcgt ggtactcgag cgggagcgga acctgcagca ggcgcgcccg
     8401 cacgtccgca gcgaccgcgc cgtgatcccg cccctggact ccggccatca cgtccatgta
     8461 ccgcttcacc gcctcgtgcc ggatcacggt cggactcgac accacgcgca cgtgggccac
     8521 gtcgcccagt cggacct
//
LOCUS       DBNGZW010000596         1964 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1350906, whole genome shotgun sequence.
ACCESSION   DBNGZW010000596 DBNGZW010000000
VERSION     DBNGZW010000596.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1350906"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="AC16JC_14120"
     CDS             <1..426
                     /locus_tag="AC16JC_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011584690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165882.1"
                     /translation="WDLGYYAWLRVLLGWPPSLLTSDILFLIPVPWVAPVLAPVIVSV
                     GLVAGSLWLLALRARGAILQFPGWIWALAAGGGALVLLSFTLDYRLVLQGHEPPAFRW
                     PLFGTGVAVGAAALVAGVRSLSGRPPEWRNVATESAPRR"
     gene            complement(401..>1964)
                     /locus_tag="AC16JC_14125"
     CDS             complement(401..>1964)
                     /locus_tag="AC16JC_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009283723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HIR5165883.1"
                     /translation="LGAWRHITPSRWPLTDVVAPAVLSVRDTIYLLSSTTSPLPILML
                     TRPATGRVEFYNRLLPWLPMARAREADVLARPDSVQPGPWDPQFFYDPDTERWFLYWN
                     SSNAYPLHAIELDKARRLAYRGTPRWLFGLDPARHGWERFGQDHRDTTTHPFIEGSWM
                     TKHAGRYYLQYGAPGTEYNVYATGVYTGDDPLGPFTYAPYNPVAYKPGGFVQGAGHGN
                     TFQDVYGNWWNTGTPWIGVNWNFERRIALHPAGFDGDGQLYVDTRFGDFPHRLPTRPW
                     RSSEELFTGWMLLSYRKTVTASSARDSFPASTVTDENPRTFWVAQRNRPGEWLTIDLG
                     RVYEVKAIQVNYADYRSGLYGTDSTVVTRFRLRASVDGTRWTTVADLSHETRDRPNAY
                     IELAAPTRARFVRYAHVHVGAANLAISDIRVFGTGAGPHPATPAGLTTRRDRDERNAT
                     VAWQPVPGAVGYNVRWGITPMKLYQTYQRFADQGTTLELRALTVGQAYWVAIESFDEN
                     GVSPLSAAVPIR"
BASE COUNT          277 a          641 c          705 g          341 t
ORIGIN      
        1 tgggacctgg gctactacgc ctggctgcgc gtgctgctcg gatggccccc gtcgctgctc
       61 acctcggata tcctcttcct tattcccgtg ccctgggttg caccggtctt ggcaccggtg
      121 atcgtcagcg tggggctggt ggcggggtcg ttgtggttgc tcgcgctgcg ggcgcgcggc
      181 gcgatactcc aattcccggg ctggatatgg gcgctcgccg ccgggggagg ggcgctcgtg
      241 ctgctgtcct tcacgctgga ctatcgcctc gtgcttcagg ggcatgagcc gccggcgttt
      301 cgctggcccc tgttcgggac cggcgtggcc gtcggcgcag ccgctctcgt cgccggagtc
      361 cggagcctgt cggggcggcc gccggagtgg cgcaacgtgg ctaccgaatc ggcaccgcgg
      421 cgctgagcgg cgacactccg ttctcgtcga agctctcgat cgcaacccag tacgcctgtc
      481 ccaccgtgag ggcgcgcagc tcgagcgtcg ttccctgatc ggcgaaccgc tggtaggtct
      541 ggtacagctt catcggcgta atcccccagc gcacgttgta gcccacggcg cccggcaccg
      601 gctgccacgc aacggtcgca ttgcgctcgt cgcggtcgcg ccgcgtggtg aggcccgctg
      661 gggtcgcagg gtgagggccg gcgccggttc cgaacacgcg gatgtcgctg atggcgaggt
      721 tcgcggcgcc cacgtgcacg tgggcgtagc gcacaaagcg ggcgcgcgtc ggtgccgcca
      781 gctcgatgta tgcgttgggc cggtcccgcg tctcgtgcga caggtcggcc accgtggtcc
      841 accgagtacc gtccaccgac gcccgtaacc ggaatctcgt caccacggtg gagtcggtgc
      901 cgtagagacc ggaccggtag tcggcatagt tcacctggat cgccttcacc tcgtagacgc
      961 ggccgaggtc gatcgtgagc cattcgccgg gacgattccg ctgcgccacc cagaaggtgc
     1021 gcgggttctc gtcggtgacc gtactcgccg gaaagctgtc gcgcgcggat gatgccgtga
     1081 cggtcttgcg gtaggacagg agcatccaac cggtgaagag ctcctcggag ctgcgccacg
     1141 gtcgcgtcgg gagccggtgc gggaagtcgc cgaagcgcgt gtccacgtag agctgcccat
     1201 cgccgtcgaa ccccgcggga tgcagtgcga tgcggcgctc gaaattccag ttcaccccga
     1261 tccacggggt ccccgtgttc caccagttgc cgtacacatc ctggaacgtg ttcccgtgtc
     1321 cggcgccctg cacgaagccg ccgggtttgt acgcgacggg attgtagggt gcgtaggtga
     1381 acgggccgag gggatcgtcg ccggtgtaga cgccggtcgc gtagacgttg tattcggtgc
     1441 cgggcgcgcc gtactggagg tagtatcgtc ccgcgtgctt cgtcatccac gagccttcga
     1501 tgaacggatg ggtggtcgtg tcgcgatggt cctggccgaa gcgctcccac ccgtgccgcg
     1561 ccggatcgag accgaacagc cacctggggg tgccccggta cgcgagacgc ctcgccttgt
     1621 cgagctcgat ggcgtgcagg ggataggcgt tggacgagtt ccagtagagg aaccagcgct
     1681 ccgtgtcggg atcgtagaag aactgcggat cccacggccc cggctgcacc gagtccggcc
     1741 tcgcgagcac gtcagcttcc cgcgcccgcg ccatggggag ccaggggagc agccggttgt
     1801 agaattccac gcgcccggtc gcggggcgag tgagcatcag aatcggcagc gggctggtcg
     1861 tactcgacag caggtagatc gtatcgcgca cggacaggac cgcgggtgcc acgacatcgg
     1921 tcagcggcca gcgcgagggc gtgatgtggc gccacgcgcc caga
//
LOCUS       DBNGZW010000597         1930 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1217398, whole genome shotgun sequence.
ACCESSION   DBNGZW010000597 DBNGZW010000000
VERSION     DBNGZW010000597.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1930)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1930)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1930
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1217398"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1349)
                     /locus_tag="AC16JC_14130"
     CDS             complement(<1..1349)
                     /locus_tag="AC16JC_14130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165884.1"
                     /translation="MSFTRVAAHAARLLLAAALLAIPQRGNAQVPQRTEKRATGAAAY
                     PAGPSRLRLARQLRLRSLTAASRTLAAPTATVTESEPNDTTTQADLVTLGDTAAGTID
                     PAGDVDVYAVDLTAGTIVSLDVFAAQGGSLLDAVLVLLGADSLTAVAFNDDFNGTDSH
                     VEYAVGTTGRYFVLIGDFNASGGAAFTYTLAFGTLSPGPGDFTTLYATGLGAPYQVAA
                     GPAGELYVADVDSLRVLKVAPNGQVSLFGTFQGELPVGVVLDGLGDVLVSTVDTNFAT
                     GRIVRFSGGVRSTFASGLESGAEMTVGPNGDVWVIDPVAALLRHYDPVGGSKGTVDLS
                     SLTNLAFEMGLAFSPAGDLYVCDGFETIYKVVNGVPQIVYQGEPFLEGIAFDKDGYLY
                     VSNGFLGRVMLLDPNLQLVGDPFARTHLGGPTQIAFLRDAGGAMTSRLLADNVGFNLV
                     "
     gene            complement(1466..>1930)
                     /locus_tag="AC16JC_14135"
     CDS             complement(1466..>1930)
                     /locus_tag="AC16JC_14135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR5165885.1"
                     /translation="ANGALSVAAGGNNVPERYGSDLWVANGYAYTGTWGRRASLGNAV
                     KIWQLGATGAPALVDSIVTTGITTVSDVEVSDDNRLLMFSAENGPNSGFWFYRLTDPA
                     HPTLIGQYLVGGISSGIHTATFSRINGRLYAFGAKDPSGAALIILDVTSLDQ"
BASE COUNT          340 a          648 c          655 g          287 t
ORIGIN      
        1 acgagattga accccacgtt gtccgccagc agccgggagg tcatggcgcc acccgcgtcg
       61 cgcaggaagg cgatctgggt cggcccgccg aggtgcgtgc gagcgaaggg gtcgcccaca
      121 agctggaggt tcgggtcgag cagcatcacg cgaccgagaa acccattgct cacgtacagg
      181 tagccatcct tgtcgaacgc gatgccctcg aggaacggct ccccctggta gacgatctgg
      241 gggacgccgt tcaccacctt gtaaatcgtc tcgaagccgt cgcagacata caggtccccc
      301 gccggggaga aggcgaggcc catctcgaat gccaggttgg tcaacgacga gagatcgacc
      361 gttcccttgg accctccgac cggatcgtag tgccgcagca gcgcggcgac cggatcgatc
      421 acccacacgt caccgttggg gccgacagtc atctcggcgc ccgactcgag gcccgaggcg
      481 aatgttgaac gtacgccgcc gctgaaccgg acgattctcc cagtggcgaa gttggtgtcg
      541 acggtcgaaa ccaatacgtc gcccaggcca tcgagcacga ccccaaccgg gagttccccc
      601 tggaacgtgc cgaacaggct gacctgcccg ttgggtgcga ccttcagcac acgcaacgaa
      661 tccacgtcgg ctacgtacag ctcgcccgcg ggacccgcgg cgacctggta aggcgcgccc
      721 agaccggtgg cgtacagggt ggtgaaatcg ccagggcccg gggacagcgt cccgaaggcg
      781 agcgtatagg tgaacgccgc cccgcccgac gcgttgaagt ccccgatcag gacgaaatag
      841 cgacccgtcg tgccgacggc gtactcgacg tgactgtcgg tgccgttgaa atcatcgttg
      901 aaagccaccg cggtgaggct gtcggcgccg aggagcacca gcaccgcgtc gagtaacgag
      961 ccgccttgcg ccgcaaacac gtcgagtgag acgatggtcc ccgccgtgag atccacggca
     1021 tagacgtcga catcgcccgc gggatcgatc gtccccgccg ccgtatcccc caacgtcacg
     1081 agatcggctt gcgtggtcgt gtcgttcggc tccgactcgg tcaccgtggc ggtgggggcg
     1141 gccaacgtcc tggaggcggc cgtgagcgat ctcaatcgca gctgccgtgc gagccgcagc
     1201 cgcgacggac cggccggata ggcagccgca ccagtcgctc gcttttcagt gcgttgggga
     1261 acctgggcgt tcccgcgttg cgggatcgcg aggagtgccg ccgcaagcaa cagacgtgca
     1321 gcgtgcgcgg cgacgcgagt gaacgacatg ggccctccca cggtgcgcgg gggccgaaca
     1381 ctcgcggtcc gcggtcatgc tgcggtcaag tggccggcag ggcgaaagat gccccgccgc
     1441 ggccgccgcg ccagcacccg cctgcctact gatccaggct cgtcacatcc aggatgatca
     1501 gcgcggcgcc gctcgggtcc ttcgcgccga aggcgtacag ccgcccgttg atgcgggaga
     1561 aggtcgccgt gtggatgccg gaggagatgc cgccgaccag atactgcccg atcaaggtgg
     1621 ggtgtgcggg gtcggtgagc cggtagaacc agaacccgga attcgggccg ttctcggcgc
     1681 tgaacatgag cagcctgttg tcgtcggaga cctcgacgtc gctgaccgtc gtgatgccgg
     1741 tcgtgacgat cgaatcgacc agcgccggcg cgccagtggc cccgagctgc cagatcttga
     1801 cggcgttgcc caggctcgcg cggcgtcccc atgtgccggt gtaggcgtaa ccgttcgcca
     1861 cccacaggtc cgagccgtag cgctcgggga cgttgttgcc gccggccgcg acgctcagcg
     1921 cgccgttcgc
//
LOCUS       DBNGZW010000598         6317 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_327666, whole genome shotgun sequence.
ACCESSION   DBNGZW010000598 DBNGZW010000000
VERSION     DBNGZW010000598.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6317)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6317)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6317
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_327666"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..5886)
                     /locus_tag="AC16JC_14140"
     CDS             complement(22..5886)
                     /locus_tag="AC16JC_14140"
                     /inference="COORDINATES: protein motif:HMM:NF036531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HIR5165886.1"
                     /translation="MSPRSEYLDAVRRRLTQLRASLARFLSLTLGHVAWSPPPWVATL
                     RARETELRARAAANPRAFRRRALAATVGVLALVVAWQLWRQRPRAVRTAFEVTAPAVT
                     DLTLETAAPHPLRVLFEASVAPLRTAGKVLTAGVDLSPALAGTWRWIGDRELDYQPAT
                     DWPVGQRFTVSLSRNALVATTVKLDRYSFDFTTAPFTATVSSAEFYQDPVDANLKRVV
                     VKLAFSHPVDTASLAKQVSLRMEGASGGFLQGLRSLPFTVIADKKGITASILSEPLAI
                     PDKDALMRVRVDAGVRSSRGGPATEEQLEQRVPIPGLFSLAVVGAEPTLVRNERYEPE
                     QVLILSTSADVSEKDVQPSVSAWVLPVFNPATPPQQRTRPYAWTRPEIIGPEILKAST
                     PLTLTPIPAEREYTSRLAFRYDADPGRYLYMRVSKGLRSFGGYVLGQTWDQIVLIPPF
                     PKEVKILGSGSLLSLSGDRRISLYSRDIPVLVVEVGRVLPDQLQHLVTQTSLIFGNTE
                     FNSDNFDASNLTARHTDTIPLPPLAPGKAHYQPYDLGRYLSSGSGPRGLFLLTVQGYD
                     PATKQVTGPLDRRLILVTDLGLLAKKGTDGGQDVFVQSLRTGLPLAGVTVDVVGKNGL
                     TVISAESDGEGHVRFPSLRSFEREQTPVLYAAHSAQDLSFLPMDRYDRELDLSRFDVG
                     GVSNALQADKLSAYLFSDRGVYLPGDAFHIGVIVKPADWETPLSGIPLEAVIADARGL
                     EVKRERLRLSARGFDEISYTTAETAPTGTWTVSLYIVKDGRADALLGTASLQVREFQP
                     DRMSMTAHLSSESVNGWVAPDSLTARVTLENLFGTPAAGRRVTATLQLTPGFPALRGL
                     EDYRFSDPQKAKDTYSEDLPATTTDAAGAALFNLGLERFARATYRLRLITEGYEADGG
                     RSVTAEAGVVVSNQPFLVGYKPDGDLRYLHKDAERRLELVAVGPDGKRRAVRQLRLAR
                     IRVTYVSVLARQYDGTYRYQSVKKEVPVSETPLAIADTGRVVRLATGEPGDFALVVED
                     SARTELSRVDYNVAGSANLTRSLERNAELQLTLNKGDYAPGETIELQVQAPYTGAGLI
                     TIERDRVYAWRWFRATTTSSVQRIPLPSGLEGNGYVSVSFVRDVNSDEVFASPLSYGV
                     APFSVSLERRKAGITLFAPTMVKPGDSFRVHYTTDRPSRIAVFAVDEGILQVAGYRTP
                     DPLGYFFQKRALEVHTTQILDLILPEFARLMAASAPGGDQESMVGRNLNPFKRKRDKP
                     VAFWSGVRDADPAGGDFRFSVPDYFNGTLRVMAVAVNEDAVGATAVKSVIRGDFVLNP
                     NVPLMVAPGDTFEVSVGVANNVVGSGKGAKIQIALQASPQLEVLGPAAATLTIDEMRE
                     GSATFRLRARDKLGNADLIFTSRLGAKTGRIAASLSLRPSVPYRTALTAGSVRQGSAS
                     AAVDRDLFPELRTVRAGMSVLPLGLAHGLMAYLEKFPYGCTEQLVSQGVPALVLARRP
                     EFGINAAQAEATFEKVLDMLRSRQNEEGAYGLWAANAHVDDYVSVYAQHFLIDARERG
                     YPVPPEVLAQGDRYLQRLAASEGASLFDERTRAYAIYLLTREGTVTTGYATSLERRVR
                     TNYGPTWQQDLTGAFLAATYQQLHQDGPANTLIGASRFGQKRPTDYSEYDDDLTHDAM
                     LLYLLARHFPARLTALKAEAIDAIVRPIQGGAYNSLSSATAILALEAYVTATGPVAAG
                     RFTVTEGLKDGSKRVLPLPAGTFPQADVSAEAKSVAFASDAKVNVFYLLSQAGYDRTI
                     PATALSQGLEVLREYTDKSGKPVSDVTLGDELHVHLRFRALGGRPVASVALVDLLPGG
                     FEVVEQPAAASVPAGVTVDNVEAGDREGEPATTWTPTFGQAVGSWSPEYAEVREDRVN
                     VYGDADASVREFVYTVKATAVGTFAVPPAYGEGMYDRSVRAWSQPGRITVRPPR"
     gene            complement(6103..>6317)
                     /locus_tag="AC16JC_14145"
     CDS             complement(6103..>6317)
                     /locus_tag="AC16JC_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019306824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165887.1"
                     /translation="VDEVPITPEKVLKALKRRGKGDEGRVGPTAFPDIPWPEPTRVLP
                     PWEGGDGKAIDQAPTLAGGVREERQH"
BASE COUNT          990 a         2101 c         2187 g         1039 t
ORIGIN      
        1 cgatcgtcgc gcgggtcgtg ctcacctggg aggccgcacc gtgatccggc ccggttgcga
       61 ccatgcccgc accgatcggt cgtacatccc ttcgccgtag gccggcggca ccgcgaacgt
      121 gccgacggcg gtggctttca cggtgtagac gaactcccgc accgacgcgt cggcgtcccc
      181 gtacacgttc acccggtcct ctcgtacctc cgcatactcg ggcgaccacg atcccactgc
      241 ctggccgaac gtcggcgtcc aggtggtggc tggctcgcct tcacggtctc ctgcttccac
      301 gttgtccact gtgacgccgg cggggacgga cgcggcggcc ggctgttcga ctacctcgaa
      361 gccccccggc agaaggtcga ccaacgccac gctcgccacg ggccgacccc ccagagcgcg
      421 gaaccggaga tggacatgga gctcgtcgcc cagggtgacg tcgctgaccg gtttaccgct
      481 cttgtcggtg tactcccgga gcacctcgag accctgcgag agggctgtgg cgggaatcgt
      541 gcgatcgtaa ccggcttggc tcagcaggta gaacacgttg acttttgcat cgcttgcaaa
      601 ggccaccgat ttcgcttcgg ccgagacgtc tgcctggggg aacgtacccg ccgggagcgg
      661 caatacccgc ttcgatccat ccttcagccc ctcagtcact gtgaagcgtc cggcagcgac
      721 gggaccggtg gctgtgacgt aggcttcgag agccaggatg gcagtggcgc tggacaggct
      781 gttgtaagcc ccgccctgga tgggacgcac gatggcgtcg atcgcctcgg ccttcagcgc
      841 agtgagccgc gcggggaaat gccgcgcgag caggtacagc agcatcgcat cgtgcgtcag
      901 gtcgtcatcg tactcgctgt agtcggtcgg acgtttctgg ccgaagcgcg atgccccgat
      961 cagcgtattg gcgggtccgt cctggtgcag ctgctggtag gtcgccgcca ggaatgcccc
     1021 ggtgagatcc tgctgccagg tggggccgta gttggtccgc acgcgccgct cgagcgacgt
     1081 ggcgtacccg gtggtgacgg tgccctcccg ggtgagcaga tagatcgcgt aggcgcgcgt
     1141 gcgctcgtcg aacaggctcg cgccttcgga cgccgccagg cgctgcaggt acctgtcgcc
     1201 ctgcgccagc acctccggcg gcaccggata gcctcgctcc ctggcgtcga tcaggaagtg
     1261 ctgggcgtac actgacacgt agtcatccac atgagcattg gcagcccaga gcccgtaggc
     1321 tccctcctcg ttctgccgcg accgcagcat gtcgagcacc ttctcgaacg tcgcctcggc
     1381 ttgagcggcg ttgatgccga actccgggcg tcgcgcgagc accagagccg gaacgccctg
     1441 gctgaccagc tgctcggtgc agccgtaggg gaacttctcg aggtaggcca tgaggccgtg
     1501 cgccagcccc agcgggagga ccgacatacc ggcgcgcacg gtgcggagct cggggaagag
     1561 gtcgcgatcc accgccgccg acgcgctgcc ctggcgtacg ctgcctgccg tgagggcagt
     1621 gcggtacggc acggagggtc tgagactcaa ggaagcggcg atacgtccgg tcttggcgcc
     1681 gagccggctg gtgaagatga ggtcggcatt gccgagtttg tcgcgcgcgc ggaggcggaa
     1741 cgtcgccgac ccctcccgca tttcgtcgat cgtcagcgtg gcggcggccg gcccgagcac
     1801 ctcgagctgc ggcgatgcct gcagcgcgat ctggatcttc gcgccctttc ccgagcccac
     1861 gacgttgttg gcgacgccca cgctcacctc gaaggtgtcc cccggtgcca ccatgagcgg
     1921 cacgttgggg ttcagcacga aatcgccccg gatgacgctc ttcaccgccg tggcgccgac
     1981 ggcgtcctcg ttcaccgcca cggccatcac gcgcagcgtg ccgttgaagt agtcgggcac
     2041 gctgaacctg aaatcgccgc ctgccgggtc tgcgtcgcgc acgccggacc agaatgccac
     2101 cggcttgtcg cgcttgcgct tgaacgggtt gaggttgcgt ccgaccatgc tctcctggtc
     2161 accacccggg gcggaggcgg ccatcaggcg cgcgaactcg gggaggatga ggtcgagaat
     2221 ctgggtggtg tggacctcga gcgcccgctt ctggaagaag tagccgagcg ggtccggcgt
     2281 ccggtagccc gcgacttgga ggatcccttc gtccactgcg aacaccgcga tacgggacgg
     2341 ccggtcggtg gtgtagtgca ctctgaatga atcgccgggc ttcaccatgg tgggggcgaa
     2401 cagcgtgatg ccggccttgc gccgctccag gctcaccgaa aacggcgcca caccgtaact
     2461 cagcgggctc gcaaacacct cgtccgaatt cacatctcgc acgaaggaca ccgaaacgta
     2521 cccgttgccc tcgaggccgg agggcagcgg gatgcgctgc accgagctcg tcgtcgtggc
     2581 ccggaaccag cgccaggcgt acacccggtc gcgttcgatc gtgatcaatc ccgcgcccgt
     2641 gtagggcgcc tggacctgga gctcgatggt ctcgccgggc gcgtagtcgc ccttgttgag
     2701 cgtcagctgc agctccgcgt tgcgctcgag cgagcgcgtg agattcgcgg aaccggcaac
     2761 gttgtagtcc acccgtgaca gctcggtcct cgcggaatcc tcgaccacga gcgcgaaatc
     2821 gccgggttcg cccgtggcca gccgcacgac gcggcccgtg tccgcgatcg cgagcggcgt
     2881 ctcgctcacc gggacttcct tcttgaccga ctggtagcgg taggtgccgt cgtattgccg
     2941 cgccagcacc gacacgtacg tcacacggat gcgcgccaga cgcagctgcc ggactgcgcg
     3001 cctcttccca tccggcccca ctgccaccaa ttccaggcgg cgctcggcat ccttgtgcag
     3061 gtagcgcaga tccccgtccg gcttgtaacc gacgaggaac ggctgattcg acacgaccac
     3121 cccggcttcc gcggtgacgc tgcgaccgcc gtcggcctcg tacccttccg tgatcaggcg
     3181 caggcgatag gtggcccggg cgaagcgttc caggccgagg ttgaatagcg ccgcaccggc
     3241 cgcatcggtc gtggtggccg gtaggtcttc cgagtaggtg tccttggcct tctgcgggtc
     3301 ggagaagcgg taatcctcga gcccgcgcag ggcggggaag cccggcgtga gctgcagcgt
     3361 cgcggtcacc cgccgtccgg ctgccggtgt cccgaacagg ttctcgagcg tgacgcgtgc
     3421 cgtgaggctg tcgggggcga cccagccgtt gaccgactcg gaggagaggt gggcggtcat
     3481 cgacatccga tcgggctgga actcgcgcac ctgcagcgag gcggtgccga gcagcgcgtc
     3541 ggcccgcccg tccttcacga tgtacaacga gaccgtccag gtcccggtcg gcgccgtctc
     3601 cgccgtggtg tagctgattt cgtcgaatcc ccgggcggag agcctcaggc gctctcgctt
     3661 cacttcgagg ccgcgggcat cggcgatcac cgcttccagc gggatgccgg agagcggcgt
     3721 ctcccaatcc gcgggcttta cgattacgcc gatgtggaac gcatcgccgg ggagatacac
     3781 gccgcggtcg gagaacaggt aggcggacag cttgtccgcc tgcagggcgt tcgacacgcc
     3841 gcccacgtcg aagcgcgaga ggtcgagctc gcgatcgtag cgatccattg gcaggaacga
     3901 gagatcctgc gcgctgtgcg cggcgtacag caccggcgtt tgctcgcgct cgaacgacct
     3961 gaggctgggg aaccgcacgt gaccctcgcc gtcggattcg gcggagatca ccgtgaggcc
     4021 gttcttgccc acgacgtcca ccgtgacgcc ggccagcggc aagccggtgc gcagcgactg
     4081 cacgaagacg tcctgtccgc cgtcggtgcc tttcttggcc aggaggccga ggtccgtgac
     4141 gaggatgaga cgccggtcca gcggcccggt gacctgcttg gtggcgggat cgtagccctg
     4201 gaccgtaagg agaaagaggc ctcgcggacc cgagccgctc gacagataac ggcccaggtc
     4261 gtacggctgg tagtgcgcct ttccgggggc gagcggtgga agcggaatcg tgtcggtgtg
     4321 ccgcgccgtg agattcgagg cgtcgaagtt gtcgctgttg aactccgtgt tcccgaagat
     4381 caggctcgtc tgcgtcacaa ggtgctgcag ctgatcaggc aggacccgcc cgacttccac
     4441 cactaggacc ggaatgtcgc gactgtagag ggagatcctg cgatcgccgc tcaggctcag
     4501 cagcgagccg ctgcccagga tcttgacctc cttgggaaac ggcgggatga gcacgatctg
     4561 atcccacgtc tgccccagga cgtagccgcc gaacgaccgc aatcctttgc tcacccgcat
     4621 gtagaggtag cggccgggat ccgcatcgta ccggaacgcc aggcgcgagg tgtactcgcg
     4681 ctccgccggg atgggcgtga gcgtgagcgg ggtggacgcc ttcaggatct cggggccgat
     4741 gatttcgggc ctggtccaag cgtagggacg cgtccgttgc tgcggcggcg tggccggatt
     4801 gaaaaccggc aggacccatg ccgacacgct gggctggacg tccttctcgc tgacatcggc
     4861 cgacgtcgag aggatgagca cctgctcggg ctcgtagcgc tcattgcgca cgagggtggg
     4921 ctcggcgccc accaccgcga ggctgaagag gccggggatc gggacccgct gctcgagctg
     4981 ctcctccgtg gccgggccgc cgcgactgga gcgcactcct gcatccacgc gtacacgcat
     5041 cagcgcgtcc ttgtccggga tggcgagagg ctcggacaga atcgaggcag tgatgccttt
     5101 cttgtcggcg atcacggtga acggcagcga ccgcagtccc tgcaggaagc cgccgctcgc
     5161 cccctccatc cggagcgaga cctgcttggc gagcgacgcg gtatccaccg ggtggctgaa
     5221 ggcgagcttg acgaccacgc gcttgaggtt ggcgtcgacc gggtcctgat agaactccgc
     5281 cgacgacact gtcgcggtga acggcgcggt ggtgaaatcg aagctgtagc ggtccagttt
     5341 gactgtcgtg gcgaccagcg cattgcggga gagggatacc gtgaaccgct gacccaccgg
     5401 ccagtcggta gccggctggt agtcgagctc ccggtccccg atccagcgcc acgtgccggc
     5461 gagcgcggga gagaggtcga cgcccgctgt cagcaccttt cctgcggtac gcagcggagc
     5521 gaccgaggct tcgaacagga cccgcagcgg atgcggggcc gcggtctcca gggtgaggtc
     5581 ggtcacggcg ggagccgtga cctcgaacgc tgtgcggacc gcccgggggc gctgcctcca
     5641 cagttgccag gcgacgacga gggccaggac gccgacggtt gcggcgagcg cgcgtcgccg
     5701 gaaggcgcgt gggttcgccg ccgcgcgggc gcgcagctcg gtctcgcggg cccgcagtgt
     5761 cgccacccag ggcggtgggg accaggcgac gtggcccaac gtcaacgaga gaaacctggc
     5821 gagggaggcg cgtagctggg tgaggcgcct tcgcaccgcg tcgaggtact cagaccgtgg
     5881 gctcacgaaa ccctcttggg aaacgtggtt cgtcgagcgt gttggattgc gcctaaggtg
     5941 gggacgacgg cggtcgcaga caatagagac tattgcctac agctcctcct ggctgcgtcc
     6001 ggcagcgcca gtcaggggcc aggaggactc gaatcggggg cgatgacaat gaggagcacg
     6061 gacgcccccg ttcgttttgg gttggcccgt tttcgcacga tgctaatgct gccgctcctc
     6121 tcgcaccccg cccgccagcg tgggcgcctg atcgatcgcc ttgccgtcgc cgccctccca
     6181 gggcggcagc acgcgcgtcg gctcgggcca ggggatgtcc gggaacgcgg tgggacccac
     6241 tcgtccctcg tcccccttcc ctcgtctctt gagggctttc aagaccttct ccggtgtaat
     6301 cgggacttcg tctaccc
//
LOCUS       DBNGZW010000599         2625 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_724220, whole genome shotgun sequence.
ACCESSION   DBNGZW010000599 DBNGZW010000000
VERSION     DBNGZW010000599.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_724220"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="AC16JC_14150"
     CDS             complement(<1..506)
                     /locus_tag="AC16JC_14150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165888.1"
                     /translation="MRRPSLLALAWLAVATSGLAAQATDTEREAAAQILRQIDSLETR
                     LQPSRTAERLAARADAARDRLLARVEAVWTGEMQGLSDWIGRNPEVGWQEFKAFDTLT
                     AVLRRYSFQVQDSVGGLRTAFTAIWTSPAGSQGPTLGLIAEYDALRGTQGAFHGDQHN
                     AQSPVAFAA"
     gene            complement(611..2320)
                     /locus_tag="AC16JC_14155"
     CDS             complement(611..2320)
                     /locus_tag="AC16JC_14155"
                     /inference="COORDINATES: protein motif:HMM:NF013955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HIR5165889.1"
                     /translation="MSYRTALRIPLALVSGWLLGCGKDSTSPQGTPPTLQSVSPATGT
                     VGTELTLTGTDFRAGASVLLDNAPADSVQVTSATTAYALVPAGVAVGHAYAVTIRNSD
                     GTSANLSGVFTPVAPVLQYVNGATKPSGNAGSTVILEGRAFGDRQGTGQVLFSNGAGG
                     TIAAAIASAADWTNTFIVTTVPAGAASGDVVVKTGTGTSNALSFTLTTNATFSPSAIS
                     WTATTALPVGLSGHSAAFAVVGGASPTNLVYVTGGADSTYAPRNDVYYATIGTDGQLS
                     SWTTTSALPAAAAFHASVVATRANSRIKAPGFIYVLGGATDAAGTPTATIYRGTLDSL
                     SGAVTGWTTLTTTLPASVHSLGAVIFRGDLYIAGGSAAGNAPVASVYRARIDSTGALG
                     AWMAEASLPFGRSYHGFGQFGGYLYAFGGDSAVVTPNDSGYTNNATKLNQIAYAKINL
                     RTGDLAGSSWTVNASGMTKVASKHTAVIAGGTVLVTGGLYSAANTGSTEETYAQLNSD
                     GTVGSFNGATGSHTIASAGGKNLFNHAALGYVDANGVAHVLVLGGDDVNAPGKKSAEV
                     WFY"
BASE COUNT          404 a          872 c          912 g          437 t
ORIGIN      
        1 gccgcgaagg cgaccgggct ctgcgcgttg tgctgatcgc cgtggaaggc gccctgggtg
       61 ccgcgcaggg cgtcgtactc ggcgatgagg ccgagggtag gaccctgcga tccggcgggc
      121 gacgtccaga tggcggtgaa cgcggtgcgc agtccgccga cgctgtcctg aacctggaag
      181 ctgtagcgcc gcaacaccgc cgtgagggtg tcgaaggcct tgaactcctg ccagccgact
      241 tcggggttgc ggccgatcca gtcggagagc ccttgcatct ctccggtcca caccgcctcg
      301 acacgcgcca gcaggcggtc gcgtgccgcg tcggcgcggg cggcgagccg ctcggcggtc
      361 cgcgacggtt gcagccgggt ctcgagcgag tcgatctgtc gcaggatctg cgcggcggct
      421 tcccgctcgg tgtccgtcgc ctgggcggcc agccccgagg tggcgacggc gagccaggcc
      481 agggcaagaa ggctgggacg gcgcatgcgg ttcctctggt ggggggaccg cgggaagctc
      541 gtgcgaagcg tgcggggagg caaggcagtg ttgcggcctc gcctccccac actattgcct
      601 caacttacga ctagtagaac cacacctccg cgctcttctt gcccggagca ttcacgtcgt
      661 cgccgccgag cacgagtacg tgggccacgc cgttggcgtc gacgtacccc agcgccgcgt
      721 ggttgaacag attcttgccg ccggccgatg cgatggtgtg ggatcccgtc gccccgttga
      781 agctgccgac ggtgccgtcg ctgttgagtt gggcgtaggt ctcttccgtg gagcccgtat
      841 tcgcggcgct gtagagcccg ccggtgacca gcacggtgcc gcccgcgatc acggccgtgt
      901 gtttgctcgc caccttcgtc atcccgctgg cattcaccgt ccagctcgag ccggccagat
      961 ctcccgtccg cagattgatc ttggcatagg cgatctggtt gagcttggtc gcgttgttgg
     1021 tgtaccccga atcgttcggc gtaacgactg cgctgtctcc gccgaacgca tacaggtagc
     1081 cgccgaactg cccgaacccg tggtacgaac ggccgaaggg cagggacgcc tcagccatcc
     1141 acgcgccgag tgcaccggtc gagtcgatgc gggcgcgata cacgctcgcc accggggcgt
     1201 tcccggctgc ggatccgccc gcgatgtaga gatcgccgcg gaagatcacc gccccgagcg
     1261 agtgaaccga agccggaagc gtggtcgtca gggtggtcca gccggtgacc gcgccggaca
     1321 gggaatcgag ggtgccccgg tagatcgtcg cggtcggcgt gccggcggcg tccgtcgccc
     1381 cgcccagcac gtagatgaac cccggcgcct tgatgcgcga attggcccgg gtcgccacca
     1441 ccgatgcgtg gaacgccgcg gcggcaggca gcgctgaggt tgtggtccag ctgctcaact
     1501 gcccatccgt cccgatcgtc gcgtagtaca cgtcgttacg cggggcgtag gtgctgtcgg
     1561 cacctccggt cacgtacacc aggttggtcg ggctcgcacc tcccaccacg gcgaacgcgg
     1621 cgctgtgacc gctgagaccg accggcagcg ccgtcgtggc cgtccagctg atcgcgctcg
     1681 ggctgaacgt cgcattcgtc gtcaaggtga agctgagagc gttgctcgtg ccggtgccgg
     1741 tcttcaccac cacgtcgccg ctcgcagccc ccgccgggac cgtcgtcacg atgaaggtgt
     1801 tggtccagtc ggccgcactg gcgatcgcgg cggcgatcgt gccgccggca ccattggaga
     1861 acagcacctg acctgtcccc tgccgatccc cgaaggcccg accctcgagg atcacggtac
     1921 tcccggcgtt gccggatggc ttcgtcgcgc cgttgacgta ctgcagcacc ggagcgaccg
     1981 gcgtgaagac cccgctcagg ttcgccgacg tcccatcgct attgcggatg gtgaccgcat
     2041 aggcatgccc gaccgcgacg ccggccggga ccagggcata cgccgtcgtg gcgctcgtga
     2101 cctggaccga gtcggccggg gcgttgtcca gcagcacact ggcgccggcg cggaagtccg
     2161 tcccggtgag agtcaattcg gtgcccaccg ttcccgtagc ggggctgacg ctctggagag
     2221 tgggcggtgt tccttgaggc gaagtggagt ccttgccaca gccaagcagc cagcccgaga
     2281 ccagggccag aggaatcctc aatgcagtcc gatacgacat ggcgacgctc cgaaagcagg
     2341 gtgttggatt gtggtcaacc agcctttaac actccgcaaa ggtccgtgca gtcacatccg
     2401 acttccggcc ggcgaccggc ttgtgggccc ggccatggaa caggtgtgac cattggcccg
     2461 tggcccgagt gtgtatgttg cgcgtatgag cgacgggccg acggacgccg aggtcatcga
     2521 acgggtactc gacggggacg ccgaccagtt cgggatcctc gtcgatcgtt acggcgatga
     2581 gttcgcccgc tatgcgaagt acatgattgg cagcgaggac gaagc
//
LOCUS       DBNGZW010000600         2833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_667368, whole genome shotgun sequence.
ACCESSION   DBNGZW010000600 DBNGZW010000000
VERSION     DBNGZW010000600.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_667368"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..853
                     /locus_tag="AC16JC_14160"
     CDS             <1..853
                     /locus_tag="AC16JC_14160"
                     /inference="COORDINATES: protein motif:HMM:NF014342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HIR5165890.1"
                     /translation="DRLRFDFTHHGPLDEATLSRIEEEVNEHIWQNLPVATRELPYKD
                     AIAAGAMAFFTEKYGDVVRMVEVPGVSLELCGGTHVDKTGEIALFRFTHETGSQAGVR
                     RIEAITGPAAYRLARALERRLGEASGMLKTQPEHLRRRIETLLEENRRLEKRVEELLR
                     GGGGPATTGAVERIGDVELHIDESDLDDRGQIALTMDAFRDKKRRAIRVLYTKGERPG
                     IHVAVTEDLVAQGVKAGDIVKELAAGTGGKGGGRPHFASAGVGDTALLGTARAQTSTI
                     VRRFLKA"
     gene            850..1155
                     /locus_tag="AC16JC_14165"
     CDS             850..1155
                     /locus_tag="AC16JC_14165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165891.1"
                     /translation="MTLADWLDARRPAPPPTLRAHLDRRVTPAPAGGGEGALPDHLAA
                     AGRDLLQRVRSHPAAGRELALDLLAADALVTYAFEAQAEADPGRLVDLARRIAGGER"
     gene            1152..1901
                     /locus_tag="AC16JC_14170"
     CDS             1152..1901
                     /locus_tag="AC16JC_14170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HIR5165892.1"
                     /translation="MIDLHRNRPGLLVLIDPGRTDAGQAGALARGARTAGAAGVLVGG
                     SFDGTHETSRVAAAVRGAAPGLPLLLFPGSAMQLTDQVDIILFLSLVSGRNPQYLIEE
                     QVRAVPFLQRHPVPTLSTGYILIDGGRVTSVEAVSQTRPLPADKPELAAAHARAARLI
                     GMQAVYLDSGSGAERPVPPPVIAACRAALGDPAALLFVGGGIRTPAEAGAARAAGADY
                     VVIGTALERGGSGEPTLEAFVTAVRPGGERA"
     gene            1898..2470
                     /locus_tag="AC16JC_14175"
     CDS             1898..2470
                     /locus_tag="AC16JC_14175"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018954090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008968 - D-sedoheptulose 7-phosphate
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose-7-phosphate isomerase"
                     /protein_id="HIR5165893.1"
                     /translation="MTPERVRDGLQRLSALIGDVASGQADTIAAIAARYEATLRAGGT
                     LFFAGNGGSAADAQHLATEYVVRYRAQRPALRAVALTTDTSLLTAAANDMGFDEVFAR
                     QVEALGRAGDLLVLHSTSGESPNVIRAAQAARARGMGVVALLGKSGGAVRGLADLALV
                     VPAEDTARIQELHLAVEHLICEWIEERLAG"
     gene            2467..>2833
                     /locus_tag="AC16JC_14180"
     CDS             2467..>2833
                     /locus_tag="AC16JC_14180"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5165894.1"
                     /translation="MIDLTGRRAIVTGGGRGIGSATALLLARAGARVVVGYRARAADA
                     AATVRAIREAGGEATAVGGDLGEPAGARRLVDQAVAALGGVDILVVNHGIWPPEDVPL
                     AEMGDAQWEETRRANLDSVV"
BASE COUNT          393 a          934 c         1122 g          384 t
ORIGIN      
        1 ggaccgcctg cgcttcgact tcacgcacca cgggccgctt gacgaggcga cgctctcccg
       61 gatcgaggag gaggtcaacg agcacatctg gcagaacctc cccgtcgcga cccgggaact
      121 gccctacaag gacgcgatcg cggcgggtgc gatggcgttc ttcaccgaga agtacggtga
      181 cgtggtccgc atggtggaag tgccgggcgt gtcgctcgag ctgtgcggcg ggacgcatgt
      241 ggacaagacg ggggagatcg cgctgttccg gttcacgcac gagaccgggt cgcaggccgg
      301 cgtgcgccgc atcgaggcga tcaccgggcc ggcggcgtac cgcctggcgc gcgctctcga
      361 gcgccggctg ggcgaggctt cgggaatgct caagacacag ccggagcacc tgcggcgacg
      421 catcgagacg ctgctggagg agaaccggcg cctggagaag cgggtggagg agctgctgcg
      481 cggtggtggt gggccggcga cgacgggcgc cgtggaacgc atcggtgacg tcgagctgca
      541 catcgacgag tcggacctcg acgaccgcgg gcagatcgcc ttgacgatgg acgcgtttcg
      601 ggacaagaag cgccgggcca tccgggtgtt gtacacgaag ggagagcggc ccgggataca
      661 cgtggcggtc accgaggatc tcgtcgccca gggggtgaag gcgggtgaca tcgtgaaaga
      721 gttggcggcc ggcaccgggg ggaagggagg cggccgtcct cactttgcct cagcgggagt
      781 cggcgatacg gccctgctcg gcacggcccg cgcccagacg tccaccatcg tccggagatt
      841 tctgaaggca tgacgctggc ggactggctc gatgcgcgcc gcccggcgcc gccgccgacg
      901 ttgcgggcac acctggaccg gcgcgtgaca cccgcacccg cgggaggcgg ggaaggtgcc
      961 ctgcccgatc acctcgccgc cgcggggcgc gacctgctgc aacgtgtgcg gtcgcacccg
     1021 gcggccggcc gcgagctggc gctcgacctg ctggcggcgg acgcactggt cacctacgcg
     1081 ttcgaggcgc aggccgaagc ggacccgggg cgcctcgtcg acctggcacg gcggatcgcg
     1141 ggaggcgagc ggtgatcgac ctgcaccgca accgcccggg acttttggtg ctgatcgatc
     1201 ccggacgcac cgatgccggg caagccggtg cactcgcgcg gggcgcgcgg acggcgggag
     1261 cggcgggggt tctggtcggc ggctcgttcg acggcacgca cgagacgagc cgcgtcgcgg
     1321 ccgcggtccg gggcgcagca cccgggctgc cgttgctcct cttccccggc tccgccatgc
     1381 agctcaccga ccaggtcgac atcatcctct tcctctcgct cgtgtcggga cgcaatccgc
     1441 aatacctgat cgaggagcag gtgcgggccg tgccgttcct gcagcgccac cccgtgccga
     1501 cgctttcgac gggctacatc ctgatcgacg gcggccgggt gaccagcgtc gaggcggtga
     1561 gccagacgcg gccgctgccg gcggacaagc ccgagctggc ggcggcgcac gcgcgggcgg
     1621 cgcggttgat cgggatgcag gccgtgtatc tcgactccgg gagcggtgcg gagcgcccgg
     1681 ttccaccccc ggtcatcgct gcctgtcgcg ccgcgctggg agatcccgcg gcgctgttgt
     1741 tcgtgggggg aggcatccgg acgccggccg aagcgggggc ggcgcgggcg gcgggagcgg
     1801 actacgtcgt gatcggcacg gctctggagc gcggggggag cggggagccg acgctggagg
     1861 cgttcgtcac ggccgtgcgg ccgggaggag aacgcgcatg acgccggagc gcgtgcgcga
     1921 cgggctgcag cgcctgtccg cgctcatcgg ggacgtggca tcggggcaag ccgacacgat
     1981 cgccgccatc gctgcgcgct atgaggccac gctgcgggcg ggaggaacgc tgttcttcgc
     2041 cggcaatggt gggagcgcgg ctgatgccca gcatctggct accgagtacg tggtgcgcta
     2101 ccgtgcccag cggccggcac tgcgcgccgt ggcgctcacc accgacacgt ccctcctgac
     2161 cgccgctgcc aacgatatgg ggttcgacga ggtcttcgcc cggcaagtcg aggcgttggg
     2221 ccgggcaggc gatctgttgg tgctccactc gaccagcggt gagagcccca acgtgatccg
     2281 cgcggcgcag gctgcgcggg cgcgcggcat gggagttgtc gccctgctcg gcaagtcggg
     2341 cggcgcggtc cggggcctcg ccgatctggc gctggtcgtt ccggccgaag acacggcgcg
     2401 tattcaggaa ttgcacctcg ccgtcgagca cctgatctgc gagtggatcg aggagcgcct
     2461 ggcggggtga tcgacctcac cgggcgtcgc gcgatcgtga ccggcggcgg acgcggcatc
     2521 gggagcgcga cggccttgct gctcgcccgc gccggcgcgc gcgtcgtggt gggctaccgc
     2581 gcccgggccg cggatgcggc cgcgaccgtg cgtgcgatcc gggaggcggg cggggaggcc
     2641 accgcggtgg gcggtgatct gggcgagccc gccggcgcgc ggcgcctcgt ggaccaggcg
     2701 gtggcggcgc tgggcggggt cgacatcctc gttgtgaatc acgggatctg gccgcccgaa
     2761 gacgtgccgc tggcggaaat gggcgacgcg cagtgggagg aaacccggcg tgccaacctg
     2821 gacagcgtcg tgt
//
LOCUS       DBNGZW010000601         3700 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1148561, whole genome shotgun sequence.
ACCESSION   DBNGZW010000601 DBNGZW010000000
VERSION     DBNGZW010000601.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3700)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3700)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3700
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1148561"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="AC16JC_14185"
     CDS             <1..1599
                     /locus_tag="AC16JC_14185"
                     /inference="COORDINATES: protein motif:HMM:NF012958.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HIR5165895.1"
                     /translation="AAQGPRFEITIPAALRAAPLTGRVYVFVSTKADEEPRLGDHGES
                     DCVPFYGVDVSALAAGAPAVIDGTTLGYPVASLNDVPAGDYYVQALVSVYTDFHRADG
                     HTIWLHDDQWEGQQFNISPGNLISEVQHVHLDPKAGYTIQLALARAIPPVEVPPDTKW
                     VKRIKFQSKLLSAFWGRPIYLGATVLLPRDYDRDTTRRYPTVYQQGHFSLRPAFGFST
                     DSVTETAAQKAEREQRSMREPGFEFYRSWRSPGFPQMIAVTFQHPTPYFDDSYAVNSV
                     NNGPYGDAIMQELIPYLEEHFRMIRAPGARFLTGGSTGGWEALALQVYHPDFFGGTWV
                     LYPDPVDFRSYELVNIYSDTNAFVYPVAEWMWSEVPAEVEVTGQPRITVRQESQFEAV
                     LGSRARSGEQFAIWEATYGPVGPDGYTAELWDLRTGHVNRDVAEYFRTHDYDLRDYLE
                     RNWRTLGPKLRGKIHLFVGDYDTFYLNLAVYRLEEFLTRANPSAQAEFAYGRPMKPHG
                     WQPWTNAQLIRIMAAQAARGRPAH"
     gene            1743..2420
                     /locus_tag="AC16JC_14190"
     CDS             1743..2420
                     /locus_tag="AC16JC_14190"
                     /inference="COORDINATES: protein motif:HMM:NF015506.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HIR5165896.1"
                     /translation="MVVTLIESGRKKRLFSGSIPSLVLHTSIIGGAVFASLNARPSDS
                     QIAYDTTLVLIAPTPERRAAQPEPELSVPVKGFQTVSIPVIIPTNIPPVSLQEHFDPK
                     DFSGLGVEGGRADGVEPPPDQVYVESAVDEAPRLLVATPPEYPTLLRDAGITGRVLLQ
                     AVIDTTGRVDPASVKVVQSPNPGFEDSSRRWILKALFRPARIRGRAVRVLVNMPLDFS
                     ITGRSGG"
     gene            complement(2434..2658)
                     /locus_tag="AC16JC_14195"
     CDS             complement(2434..2658)
                     /locus_tag="AC16JC_14195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165897.1"
                     /translation="MAKPIVSLAAAGVLGFALWKVLSFLFLPLVGTLLGVLFTLVKVA
                     AIVGVVLLVMWLLRGKKDDTQRDDAQKGEA"
     gene            complement(2684..3508)
                     /locus_tag="AC16JC_14200"
     CDS             complement(2684..3508)
                     /locus_tag="AC16JC_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013250207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HIR5165898.1"
                     /translation="MSDTIKQTVREKYGQAALRVLAGSGSSCCGAGAAGESCDPVTSN
                     LYAPGDTAGLPETAVLASLGCGNPTALAQLKPGDVVLDLGSGGGIDVLLSARLVAPGG
                     KAYGLDMTDEMLALARENQRRAGVENVEFLKGDIEHIPLPDASVDVIISNCVINLAAD
                     KRRVLAEAFRVLKPGGRFAVSDVVVRGTLPAEVRRNMELWVGCVAGALEEEEFKGLLT
                     ASGFTDVSIEPTRVYAFEDARAFLAGTGLDADVLAREIGGRVMGAFVRATKPSDTF"
BASE COUNT          625 a         1304 c         1187 g          584 t
ORIGIN      
        1 gccgcccagg gcccgcgctt cgagatcacg attccggccg ccctgcgcgc ggccccgctg
       61 accgggcgcg tgtacgtctt cgtctccacc aaggccgacg aggagccccg cctcggtgac
      121 cacggcgaga gcgactgcgt ccccttttac ggcgtggacg tgtcggcgct ggcggccggc
      181 gccccggcgg tgatcgacgg cacgacgctc ggctacccgg tggcgagtct caacgacgtc
      241 cccgcgggcg actactacgt ccaggcgctc gtcagcgtgt acaccgactt tcaccgggcg
      301 gacggacaca cgatctggct gcacgacgac caatgggagg gccagcagtt caacatctcc
      361 cccgggaacc tgatcagcga ggtgcagcat gtgcatctcg accccaaggc cggctacacg
      421 atccagctgg cgctcgcccg cgccatccct ccggtcgagg tgccgcccga cacgaagtgg
      481 gtgaagcgca tcaagttcca gagcaagctc ttgtcggcat tctggggccg gccgatctac
      541 ctcggcgcca cggtgctgct gccgcgcgac tacgaccgtg ataccacgcg ccgctacccc
      601 acggtctacc agcaaggcca cttctcgttg cgcccggcgt tcggattctc caccgacagc
      661 gtgacggaga cggccgcgca gaaagccgag cgcgagcagc gctccatgcg cgagcccggg
      721 ttcgagttct accgctcatg gcggtccccc ggcttcccgc agatgatcgc ggtgacgttc
      781 cagcatccca ccccctattt cgacgactcc tacgccgtca actcggtgaa caacggcccc
      841 tacggcgacg ccatcatgca ggagctgatc ccctacctgg aggagcactt ccgcatgatc
      901 cgcgcgcccg gggcgcgttt tctcaccgga ggatcgaccg gtggatggga ggccctggcg
      961 ctgcaggtct atcatccgga cttcttcggc gggacgtggg tgctgtatcc cgatcccgtg
     1021 gatttccgca gctacgagct ggtcaacatc tacagcgaca cgaacgcgtt cgtctatccg
     1081 gtggcggagt ggatgtggtc cgaagtcccc gccgaagtcg aggtgaccgg ccagccgcgc
     1141 atcacggtgc ggcaggagag ccagttcgag gccgtgctcg gcagccgtgc ccgctccgga
     1201 gagcagttcg cgatctggga ggccacctac ggaccggtgg gaccggacgg ctacacggcg
     1261 gagctgtggg acctgcgcac ggggcacgtc aatcgtgacg tggcggaata cttccgcacc
     1321 cacgactacg acctgcgcga ctacctcgag cgcaactggc gtacgctcgg gcccaaactg
     1381 cggggcaaga tccacctctt cgtgggcgac tacgacacct tttatctcaa cctggcggtg
     1441 taccgcctcg aggagttcct gacgcgcgcg aacccatcgg cgcaggccga gttcgcgtac
     1501 ggccggccga tgaagccgca cggctggcag ccgtggacca acgcgcagct gatccggatc
     1561 atggcggccc aggcggcacg cggccgtcca gcccactgag cgtccgacga catcctgaca
     1621 ccaggcgaac accatcgccg caaccagctg ctatcctccg gcgttctcat acctgagacg
     1681 ccgggggatg ccgtcccacg actctccggc cgcgttccaa aacggagcat cggaggacgg
     1741 ctatggtagt cacgctcatc gagtccgggc ggaagaaacg cctgttcagc ggcagcatcc
     1801 cctcgctggt gctgcacacc tccatcatcg gcggtgcggt cttcgccagc ctgaacgcgc
     1861 gtccgagcga ctcgcagatc gcctacgaca cgacgctggt gctgatcgca ccgacaccgg
     1921 agcggcgcgc ggcacagcct gagcccgagc tcagcgtgcc ggtgaagggg ttccaaacgg
     1981 tgagcattcc ggtgatcatc cccacgaaca tcccgcccgt atcgctgcag gagcacttcg
     2041 atcccaagga cttctcggga ttgggcgtcg agggtggtcg cgccgatggc gtcgaaccgc
     2101 ctccggatca ggtatatgtg gagagcgccg tggacgaggc gccgcggttg ctggtcgcga
     2161 ctccacccga gtatcccacg ctgctgcgcg atgccgggat caccggccgc gtgctgttgc
     2221 aggccgtgat cgacacgacg ggaagagtgg accccgcttc cgtgaaagtg gtgcagagcc
     2281 cgaatcccgg attcgaggat tccagtcgcc gctggatcct gaaagcgctg ttccgtcccg
     2341 cgcgcatccg gggccgggcg gtccgggtgc tggtcaacat gccgctcgat ttctcgatca
     2401 ccggccgcag cggcgggtga cgacccaggg ggtctaggct tcgcccttct gggcgtcgtc
     2461 cctctgcgtg tcgtccttct tcccccgcag gagccacatc acgagcagca ccacgcccac
     2521 gatggccgcc accttgacca gcgtgaacag cactcccagg agtgtgccga cgagcggcag
     2581 gaagaggaac gagaggacct tccagagggc gaagccgagc acgccggccg ccgcgagcga
     2641 aacgattggt ttggccatgg tcgctcccct acggccccag cggctaaaag gtgtccgacg
     2701 gcttggtggc gcgcacgaag gcccccatca cgcgtccgcc gatctcccgg gcgagcacgt
     2761 cggcgtcgag ccccgttccg gcgagaaacg cccgagcgtc ctcgaaggcg tacacgcggg
     2821 tcggttcgat tgagacatcg gtgaagccgc tcgcggtgag cagccccttg aactcctcct
     2881 cctcgagcgc tccggccacg cacccgaccc acaactccat gttacgccgg acctccgctg
     2941 gcagggtccc gcgcaccacg acgtcggaca cggcgaagcg gccgcccggc ttcaggaccc
     3001 ggaacgcctc ggccaggacg cggcgcttgt cggcggcgag gttgatcacg cagttggaga
     3061 tgatgacgtc caccgacgcg tcgggaagcg gaatgtgctc gatgtcccct ttgagaaact
     3121 ccacgttttc cacgccggcc cggcgctggt tctcacgcgc gagggcgagc atctcatcgg
     3181 tcatgtcgag gccgtaggct ttccctccgg gcgccacgag acgcgccgag aggagcacgt
     3241 cgatcccgcc gcccgaaccc aggtcgagca ccacgtcgcc cggcttcagc tgggccaggg
     3301 ccgtcgggtt gccgcagccc agggaagcga gaacggcggt ttccggaagt ccggcggtat
     3361 cccctggcgc atagaggttg gacgtcacgg ggtcgcagct ctctccggct gcacccgcgc
     3421 cgcagcagct cgagccgctc ccggccagca cgcggagcgc ggcctggccg tacttctccc
     3481 gcactgtctg cttgatcgtg tcgctcatgt ctgggcctcg tctatcaaga aatgttgatg
     3541 cgtacggcaa aaaaaacccc tcatgcacag cacccggcac ggcagctccc ggcgtgcttc
     3601 ccgggctgca ccgccgcggc gaatgcagcg atctcctcca gcgcttcgcg attcagggaa
     3661 tagtgcaccc agcggccgtc cttgcggtcg gtgacgaacc
//
LOCUS       DBNGZW010000602         5170 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_724485, whole genome shotgun sequence.
ACCESSION   DBNGZW010000602 DBNGZW010000000
VERSION     DBNGZW010000602.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5170)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5170)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5170
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_724485"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="AC16JC_14205"
     CDS             <1..740
                     /locus_tag="AC16JC_14205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004272348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5165899.1"
                     /translation="GGLADALEAIVTQRGAETIAAVIVEQVAGSTGVLVPPRGYLERL
                     RALCDRHGILLIFDEVITSFGRLGAPFAAQALGVLPDMISCAKGITNGAVPMGAVAVR
                     RGIHDAIVDSAPAGIALFHGYTYSGHPLATAAGLATLELYRTEGLFERARILAPAWED
                     AVHALRGLPHVVDLRNLGLMAALELEPRSGAPGARGFELFLDCFQHGALVRATGDVIA
                     FSPPLVIERGQIDELFGLVADALRRLP"
     gene            737..1948
                     /locus_tag="AC16JC_14210"
     CDS             737..1948
                     /locus_tag="AC16JC_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017462771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5165900.1"
                     /translation="MSRRSDHRSATLAGFLGWTLDAFDFFLVVMTATAIAAEFGKTDK
                     DVFFTLTLTLGFRPVGAFIFGLVADRYGRRLPLMIDLVFYSIVEVLSGLAPNYTTFCV
                     LRALFGIGMGGEWGVGASLVMEKVPVGRRGLWSGLLQQGYALGYLLAALCYFVVFPRW
                     GWRPLFFIGGLPALLALYVRSRVSESEVWQRSRATSWSHLGRVLLANWKTFAYLVVLM
                     ALMNGVSHGTQDLYPTLLKRDWGFQTPRVVGLTAISMVGALIGGVLAGHYSDRMGRRR
                     TIVMALCSALLLIPLWAFSPNVFLLAAGAFLMQVMVQGAWGVIPAHITELSPDATRGV
                     LPGFAYQCGAAISGVMPWLGGYIAEQTGYARAMAMTAGVVFVLCAIAAWFGPERRGIV
                     YGAGQAVSDPA"
     gene            complement(1932..3026)
                     /locus_tag="AC16JC_14215"
     CDS             complement(1932..3026)
                     /locus_tag="AC16JC_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013567372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR5165901.1"
                     /translation="MPTTAAPVMAAATLLRGLGRAIGNTPLLAIRCRFRGRERLIYAK
                     AEHLNLTGSIKDRMALHILATAYGSGALQPGDTIAEATSGNTGIAFAALGRALGHPVT
                     IFMPEWMSRERHELIRSLGASVVPVSREEGGFLGSIQMAETLATRQPHVFLPRQFENT
                     ANVTAHERTTAPEIWWQLEAAGLVPDAFAAGVGTGGTVMGVGRFLRARRAEARVHPIE
                     PAESPTLSTGHKVGRHRIQGMSDEFVPAVVDLTQLDGVIAVSDGDAILMAQELASRLG
                     LGVGISSGANFLGALLVQEELGPDAVVVTVFPDDNKKYLSTGLLQEEPVRSDYLAPRV
                     ELLGFDALRRSCRACIAQELGDLQEVTPGR"
     gene            complement(3081..4631)
                     /locus_tag="AC16JC_14220"
     CDS             complement(3081..4631)
                     /locus_tag="AC16JC_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017877876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR5165902.1"
                     /translation="MRTPPCWALVTVASAIAAAPAAAQVALPRDLDAEVTRALRLFQV
                     PGMAIAVVKDGKVLLARGYGVRRMGDPAPVDANTYFQIASNTKAFTTALLAQLVDSGR
                     LAWDDPVTKYLPWFQLWDPWVTREFTIRDLVTHRSGLGLGAGDLLWFHSNYGAEEVVR
                     RLRAAKPVTSFRTAYAYDNVLYIAAGLVIEAVTGRPWSAVVRDRIFTPLGMTAAGTDI
                     ALMSSADAATAHSMIGGPLHIVPLDTIDNTLPAGGIIANVTDLAKWMIARLDSGAIPG
                     GGRLFSVRQAAEMWTAQTILPIGDPPPPLAALRANFAAYGLGWSLRDYRGHKLVSHDG
                     GLAGMTSRTLLVPDLKLGIVMLTNAEQPVYGPLGFELLDHFLGAPRTDWITRIHAVMA
                     QGQAGADSMQRAQAAQRDSTSHPSLPLARYAGAYVDAIYGDVTIALEGDHLVLRFSHS
                     PALVGDLGHWQYDTFVAHWRSAHIEDAYVTFSLHPDGTIEHFKMAAVSPLADFSFDYQ
                     DLLFVPAP"
     gene            complement(4648..>5170)
                     /locus_tag="AC16JC_14225"
     CDS             complement(4648..>5170)
                     /locus_tag="AC16JC_14225"
                     /inference="COORDINATES: protein motif:HMM:NF024822.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4112 domain-containing protein"
                     /protein_id="HIR5165903.1"
                     /translation="ALGQDYCLQSVEHPRSRWRDMLTPEQRLARVQRVARLWDGIIRI
                     PGTPFAIGLDPLLGLVPGAGDALAAGVTGWLVLEAARLGVPASTLVRMLTNVAIDALI
                     GAIPVAGDVFDFAWKANLRNVALLERHMAEPAAARRAGRGWLLAIGAGLTLLLAGTIV
                     LGVLTVRALLRRG"
BASE COUNT          740 a         1783 c         1821 g          826 t
ORIGIN      
        1 ggggggggct cgctgatgcc ctggaagcga tcgtgacgca gcgcggcgcg gagacgatcg
       61 cggcggtgat cgtggagcag gtggccggct cgaccggcgt gctggtccct ccgcgcggct
      121 acctcgagcg gctgcgcgcg ttgtgcgacc ggcacggtat cctgctgatc ttcgacgagg
      181 tgatcaccag cttcggccgc ctcggggctc cgttcgccgc gcaggcgctc ggcgtgctcc
      241 cggacatgat cagctgcgcc aaggggatca ccaacggcgc ggtgcccatg ggcgccgtgg
      301 cggtgcggcg cggcatccac gacgcgatcg tcgacagcgc cccggcgggc atcgcgttgt
      361 tccacggcta cacctattcg ggtcacccgc tcgcgaccgc ggccgggctc gcgacgctcg
      421 agctgtaccg gaccgaaggc ctgttcgagc gcgcccggat cctggcgccc gcctgggagg
      481 acgcggtgca cgcgttgcgc ggactgccgc acgtcgtgga cctgcgcaat ctcggcctga
      541 tggcggcgct ggagctcgag ccgcgctccg gtgccccggg cgcccgcggg ttcgagctgt
      601 tcctcgactg cttccagcac ggcgcgctgg tccgggcgac cggagacgtc atcgcctttt
      661 cgccgccgct cgtcatcgag cgcgggcaga tcgacgagct gttcgggctg gtcgcggacg
      721 cgctgcggcg cctgccatga gccggcgaag cgaccaccga tcggctaccc tggccgggtt
      781 cctcggctgg acgctcgacg cgttcgattt cttcctcgtg gtgatgaccg ccacggcgat
      841 cgcggcggag ttcggcaaga cggacaagga cgtcttcttc acgctgacgc tcaccctcgg
      901 gttccgtccg gtgggcgcgt tcatcttcgg cctcgtggcc gaccggtacg gccggcgcct
      961 cccgttgatg atcgacctcg tgttctattc gatcgtcgag gtgctctccg gtctggcccc
     1021 gaattacacg acgttctgcg tgctgcgggc gctgttcggc atcggcatgg gcggcgagtg
     1081 gggagtcggc gcctcgctgg tgatggagaa ggtgccggtc ggccggcgcg ggctctggtc
     1141 gggattgttg cagcagggct acgcgttggg ctatctgctc gccgcgctgt gctatttcgt
     1201 ggtgttcccc cgctggggct ggcgccccct gttcttcatc gggggactgc cggcgctgct
     1261 cgcgctgtac gtgcgttccc gggtctcgga gtccgaagta tggcagcgct cgcgggcgac
     1321 ctcctggagc catctcggcc gggtgctcct cgccaactgg aagacgttcg cgtacctcgt
     1381 cgtgctcatg gccctgatga acggcgtgtc gcacggcacc caggacctct accccaccct
     1441 gttgaagcgc gattggggct tccagacgcc gcgcgtcgtc ggactgacgg cgatctccat
     1501 ggtcggcgcc ctgatcggcg gggtcctggc cggccattac tccgaccgga tggggcgccg
     1561 ccggaccatc gtcatggcat tatgcagcgc cctgctgctg atccccctgt gggcgttttc
     1621 ccccaacgtc ttcctgctcg cggctggagc gttcctcatg caggtgatgg tgcagggagc
     1681 ctggggggtg attcccgcgc acatcaccga gctctccccg gacgcgacac ggggagtctt
     1741 gccggggttc gcctaccagt gcggggccgc catctccggc gtgatgcctt ggctcggcgg
     1801 gtacatcgcc gaacagacag ggtacgcccg ggcgatggcc atgacggccg gcgtggtctt
     1861 cgtgctgtgc gccatcgccg cctggttcgg acccgaacgg cgcgggatcg tctacggcgc
     1921 cggccaggcg gtcagcgacc cggcgtgacc tcctggagat cgcccagctc ctgagcgatg
     1981 cacgcgcgac acgagcggcg cagcgcgtcg aacccgagca gctcgacgcg cggggcgagg
     2041 tagtcggagc gcaccggctc ttcctgcagc aacccggtgc tgaggtactt cttgttgtcg
     2101 tccgggaaga cggtgaccac cacggcgtcc ggtcccagct cctcctggac cagcagcgcc
     2161 ccgaggaaat tcgccccgga ggagatcccc acccccaatc ccagacgtga cgcgagctcc
     2221 tgcgccatca aaatggcgtc gccgtccgac accgcgatca cgccgtcgag ctgcgtcagg
     2281 tccaccaccg ccgggacgaa ctcgtcggac atcccctgga tgcggtgccg tcccactttg
     2341 tgcccggtgc tcagggtggg cgactcggcg ggctcgatcg ggtgcacccg cgcctcggcc
     2401 cgtcgcgcgc gcaggaagcg cccgaccccc atcaccgttc cccccgtgcc cacccccgcc
     2461 gcaaaggcgt ccggcaccag ccccgccgcc tccagctgcc accagatctc gggcgcagtg
     2521 gtgcgctcgt gcgccgttac gttggccgta ttctcgaatt gccgcggcag gaacacgtgc
     2581 ggctgacgcg tcgccagcgt ctcggccatc tggatgctgc ccagaaaccc gccttcctca
     2641 cgactcaccg gcacgacgga cgcaccgagc gagcggatca gctcgtgccg ctcccgactc
     2701 atccattccg gcatgaagat cgtcaccgga tggccgagtg cccgtcccag cgccgcgaac
     2761 gcgatcccgg tgttgccgct ggtcgcttcc gcgatggtgt cgccgggctg cagggcgccc
     2821 gatccgtaag cggtcgcgag gatgtgcagc gccatccggt ccttgatgct gccggtcagg
     2881 ttcaggtgct cggccttggc gtagatcagg cgctcgcggc cgcggaaccg gcagcggatg
     2941 gcgagcagcg gcgtgttgcc gatggcgcga cccagtcccc gcagcagggt ggccgcggcc
     3001 ataacggggg cggcggttgt cggcatggtt cgagagtggc gcggccgcca tgaagctccc
     3061 atgaggtgaa gatgaaggcc ctagggggcg ggcacgaaga gcaggtcctg atagtcgaag
     3121 ctgaagtcgg ccagtgggct caccgcggcc atcttgaaat gctcgatcgt cccgtcggga
     3181 tgcagcgaga acgtcacgta ggcatcctcg atatgggccg accgccagtg cgccacgaac
     3241 gtgtcgtact gccagtgccc caggtcgccg acgagcgccg gagagtggga gaaccgcagc
     3301 accaggtggt cgccctcgag ggcgatggtc acgtccccgt agatggcgtc gacgtaggcg
     3361 cccgcgtagc gcgccagcgg cagcgacgga tgcgaggtgg agtcgcgctg tgccgcctgc
     3421 gctcgctgca tcgagtccgc gccggcctgg ccctgcgcca tgacggcgtg gatgcgcgtg
     3481 atccagtcgg tccgcggcgc gcccaggaag tgatcgagca gctcgaagcc aagcggcccg
     3541 tacacgggtt gctcggcgtt ggtgagcatc acgatcccga gcttcagatc gggcacgagc
     3601 agagtgcgcg acgtcatgcc ggcgaggccg ccgtcgtgcg acacgagctt gtgcccccgg
     3661 tagtcgcgca gcgaccaccc caatccgtag gccgcgaagt tggcccgcag ggcggcgagc
     3721 ggcggcggtg gatccccgat cgggaggatc gtctgcgccg tccacatctc cgccgcctgc
     3781 cgcacgctga acaggcgccc cccgcccggg atcgcccccg agtcgagccg cgcgatcatc
     3841 cacttcgcga ggtcggtcac gttggcgatg ataccgccgg ccgggagggt gttgtcgatc
     3901 gtgtcgagcg gaacgatgtg cagcgggccg ccgatcatgc tgtgggccgt cgcggcatca
     3961 gcggagctca tgagagcgat gtcggtgccg gcggcggtca tgccgagcgg ggtgaagatg
     4021 cggtcccgca cgaccgcact ccacggtctt cccgtcacgg cttcgatcac gagaccggcc
     4081 gcgatgtaga gcacgttgtc gtaggcgtac gccgtccgga agctcgtcac cggcttggcc
     4141 gcacgcaggc ggcgcacgac ctcttccgcc ccatagttcg agtgaaacca gagaaggtcc
     4201 cccgctccga ggccgagccc cgagcggtgc gtcacgagat cgcgaatcgt gaactcgcgg
     4261 gtcacccagg gatcccacag ctggaaccac ggcaggtact tcgtgaccgg atcgtcccag
     4321 gcgagccggc ccgaatcgac cagctgggcg aggagggcgg tcgtgaacgc cttggtgttc
     4381 gaggcgatct ggaagtaggt gttcgcgtcc accggggccg ggtcgcccat gcggcgcacg
     4441 ccgtagccgc gcgccagcag gaccttgccg tccttcacga cggcgatcgc catccccggg
     4501 acctggaaca ggcgcagcgc acgcgtcact tcggcgtcga gatcgcgcgg cagggcgacc
     4561 tgggccgcgg ccggggccgc ggcaatggcg ctcgcgacgg ttacgagtgc ccagcacggt
     4621 ggcgtcctca tcgattcacc tccggtttta tcctcggcgc aacagcgctc gaacggtgag
     4681 cacgccgagt acgatggtgc cggcgaggag cagcgtcagc cctgcgccga tggcgagcag
     4741 ccagccgcga ccggcgcgac gtgcggccgc cggttcggcc atgtgacgct cgagcagtgc
     4801 gacgttcctg agattcgcct tccaggcgaa atcgaacacg tcgccggcca cgggaatcgc
     4861 gccgatcagc gcatcgatcg cgacgttggt gagcatccgc accagcgtgc tcgcgggcac
     4921 gccgaggcgg gccgcttcga gcacgagcca gcccgtgacg ccggcggcca gcgcatcccc
     4981 cgcgccggga acgagcccca gcagcgggtc gaggccgatc gcgaacggcg taccgggaat
     5041 ccggatgatg ccgtcccaca ggcgcgccac tcgctggacg cgtgcgagac gctgttcggg
     5101 ggtcagcatg tcacgccacc tcgatcgggg atgctcgacg gactggaggc agtagtcttg
     5161 tccgagagca
//
LOCUS       DBNGZW010000603         4784 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_56293, whole genome shotgun sequence.
ACCESSION   DBNGZW010000603 DBNGZW010000000
VERSION     DBNGZW010000603.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4784)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_56293"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="AC16JC_14230"
     CDS             complement(<1..395)
                     /locus_tag="AC16JC_14230"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR5165904.1"
                     /translation="MIFHHTADSHVLELPFVGEVHLPTGWYLFGVDISPTRHVFFMLV
                     AALFILATVWYAGWQTQRRHREGKAPRGFGAAIEAVVLFVRNDIAIGNIGHNGAKFAP
                     YILTLFFFVLYCNLLGLFPWGATPTGNIVV"
     gene            complement(419..748)
                     /locus_tag="AC16JC_14235"
     CDS             complement(419..748)
                     /locus_tag="AC16JC_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165905.1"
                     /translation="MKGYGALLGLGLVLVAVAGWVGGRGAVIGGAVALAAQLGAVALL
                     RPAMAAPHRVFMARWLGGMGVRALSLGGLLAVAVTHRAPLPLLATTLGFLGVLLPLLF
                     LEIRFLK"
     gene            complement(745..1011)
                     /locus_tag="AC16JC_14240"
     CDS             complement(745..1011)
                     /locus_tag="AC16JC_14240"
                     /inference="COORDINATES: protein motif:HMM:NF021073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR5165906.1"
                     /translation="MAPDDPNNDSRSYGQGYEYVAMGFGFAFAILGFGALGWLVDGWL
                     HTRPLFAIAGGFLGGFGGFMSIYYRVTRETQADKKRRGQGTGDG"
     gene            1118..2158
                     /locus_tag="AC16JC_14245"
     CDS             1118..2158
                     /locus_tag="AC16JC_14245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012528352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5165907.1"
                     /translation="MTTTPRPAPTAPSAASPGDVELLERLAAGRRALVDQVAQRIVGQ
                     RDVVDGILTAVFSGGHALLVGVPGLAKTLMVQTVAEALELKFSRIQFTPDLMPSDITG
                     TEIIEEDTATGKRSFRFVRGPVFANIVLADEVNRTPPKTQAALLQAMQEHQVTAAGQT
                     HSLPAPFFVLATQNPIEQEGTYPLPEAQLDRFMFELRVGYPSREEEETIVDATTADYA
                     TKITPVLTAEDVLALQHLVRRIPATKPLVRAAVTLVRMTRPDDPGAPEFVREYISWGA
                     GPRASQYLVLGAKALAALDGRPSPDYEDLKRVAPLVLTHRLVINFAAEAAGKSAPDLV
                     GELVSGGAWMPK"
     gene            2324..>4784
                     /locus_tag="AC16JC_14250"
     CDS             2324..>4784
                     /locus_tag="AC16JC_14250"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR5165908.1"
                     /translation="MIHQVVPHLRLLTLGAPLLVTTTNEAVRYRTRKHFALLIRLALE
                     PGRRFARDYLIDLLWPDVPTERGRHSLSQAVTVLRQTVGPQNLAAHRTSIALADGVVE
                     TDALQLEAGEPEIRGRFLEGFEVRGARGFDEWREGWVAQLHPRIRDCLVKRMDAARRI
                     GDFATVERHARVLEEVDPVSEDAVRGVMEARAWVGDRSSALKAFARFAARLAEDLKAK
                     PSAELQRIADLLREGRRAAPRPTADGVPARQERRFEAETILGRQREFSTLYDAWLEVR
                     QSRSRVIVILGEPGIGKTTLTNAFISSCQMEGGIIARTQAYDAERELPYAVLAELVRQ
                     LTLQRAIGAAEPEALAELTRLTPEVLSAFPGVPRPDEWPPEVVPLRLADAFFKAVMAA
                     TDEFPVVLIVDDIHAADNASVAILHMLARKLAGLRVLLIVTGRPGSLRPPSSAHALVT
                     DSVIEGLGTVELDALSNEAAALLVARASAKGIERWGEPPIDRIVRAGGGNPLALELLS
                     RDWVENGADSLVGKLDPVHPLPAGLQSIPKAIRTMVERQTEALAERTRAVFDLASVLG
                     RRMGDLWLYQVIGCSETEALYQLGQLLEARLLRDIAGTLEFRNELIRAHAYYEIPSTV
                     RLELHRRIAGVLEAQGGNDANHLEIAWHYILGRDLERAIPVALSGAEQSLRAGAPREA
                     ELILRRLMREKVDARRAGRMELFLSEALLSQSKADEALSILDQRLGDQTLTKPEVAQA
                     ARLYASGVYLVNKNSAARHSEMAKRALQVAHECGDPDLLAYALFESGRAGVESGDQGL
                     TRTTRQAVRAIIAEEDGNAPSI"
BASE COUNT          834 a         1601 c         1601 g          748 t
ORIGIN      
        1 accacgatgt tcccggtggg cgtggcgccc cacgggaaga gacccagcag gttgcagtag
       61 agaacgaaga agaaaagggt caggatgtag ggcgcgaact tggcgccgtt atgcccgatg
      121 ttgccgatgg cgatgtcgtt gcggacgaac agcaccaccg cttcgatcgc ggcgccgaac
      181 ccccggggcg ccttgccctc gcggtggcgc cgctgggtct gccacccggc gtaccacacc
      241 gtggccagaa tgaagagcgc cgcgacgagc atgaagaaca cgtgccgcgt cggcgaaatg
      301 tccacgccga acaggtacca cccggtcggc agatgcacct caccgacgaa cggcaactcc
      361 agcacatggc tgtccgccgt gtgatggaag atcatcgcgc cgatatcggg ttcttgcatc
      421 atttcaggaa cctgatctcc aggaacagca gcggtaagag cacgcccaga aacccgagtg
      481 tcgtcgccaa cagcggcagc ggggcccgat gcgtcaccgc caccgccaac aacccgccga
      541 gcgacagcgc gcggaccccc atcccgccaa gccagcgcgc catgaacacc cggtgcggcg
      601 ccgccattgc gggccggagc agggccactg ccccgagctg cgccgcgagc gccacggcgc
      661 ccccgatcac cgcgccacga ccccccaccc accccgccac cgccaccagg acgagtccga
      721 gtcccaacag ggcaccgtag cccttcaccc gtcccccgtc ccctgccccc gtcgcttctt
      781 gtccgcctgg gtttcgcgcg tcacgcggta gtagatgctc atgaagccgc cgaacccgcc
      841 gagaaacccc ccggcgatcg cgaacagcgg ccgcgtgtgc agccagccgt ccaccagcca
      901 ccccagcgcc ccgaacccga gaatcgcgaa ggcgaaaccg aaccccatcg cgacgtactc
      961 gtagccctgg ccgtagctgc gagaatcgtt attcgggtcg tccggtgcca tcgcgacgcg
     1021 cgaaagctac gtgcttgtga aatttttcgc aagcaaagcc cttggggtgc aactttatgt
     1081 ggcacttggg agtacgatgt gatatcctac gcactccatg accacgaccc cccgccccgc
     1141 acccaccgct ccatcggccg catcgccggg cgacgtcgag ttgctcgaac ggctcgccgc
     1201 cggacgccgc gccctggtgg accaggtcgc gcaacgcatc gtgggccagc gcgacgtggt
     1261 ggacggcatc ctcaccgccg tcttctctgg gggccacgcg ctcctggtgg gcgtgcccgg
     1321 gctcgccaag accctgatgg tgcagacggt ggcggaggcg ctcgagctga agttctcgcg
     1381 catccagttc acccccgacc tcatgccgtc cgacatcacc gggacggaaa tcatcgaaga
     1441 agacaccgcc acgggaaagc gcagcttccg cttcgtgcgc gggcccgtgt tcgcgaacat
     1501 cgtgctggcc gacgaagtga accgcacgcc gcccaagacc caggccgcgc tgctgcaggc
     1561 gatgcaggag caccaggtga ccgcggcggg tcagacgcat tccttgcccg cgccgttctt
     1621 cgtactcgcc acccagaatc cgatcgagca ggagggcacc tatccgctcc ccgaggcgca
     1681 gctcgaccgc ttcatgttcg agctgcgggt ggggtatccg tcgcgtgagg aagaagagac
     1741 gatcgtagac gcgacgacgg cggactacgc gacgaagatc acgccggtgc tgacggccga
     1801 ggacgtgctc gcgctgcagc atctggtccg gcgcattccc gcgaccaagc cgctggtgcg
     1861 cgcggcggtg acgctggtgc gcatgacccg gcccgacgat ccgggagcgc ccgagttcgt
     1921 gcgggagtac atctcctggg gggcgggccc gcgggcgtcg cagtacctgg tcctcggcgc
     1981 caaggcgctg gcggcgctgg acgggcggcc gagtcccgat tacgaagatc tgaagcgggt
     2041 ggcgccgctg gtattgacgc accggctggt gatcaacttc gcggccgagg cggcggggaa
     2101 gtcggcaccg gacctggtcg gcgagctcgt ctcaggcgga gcctggatgc cgaaatagcc
     2161 cccgttccgt ttggtccgtc cgccctcggc cccttcgggc ttttttgaaa ttgtcgattt
     2221 tgcctgcctt tcaatgccac gaaaagcatt gccactgcat atgttgacat tgttttgaac
     2281 acgccacctt attgtctccg gagccgtcgg caggggtggc tgtgtgattc accaggtcgt
     2341 tccccatctg cgcctactca ccctgggcgc cccgctactc gtcaccacta ccaacgaggc
     2401 agtgcgctac cgcacgcgca agcacttcgc actgctgatc cgtctcgcgc tcgagcccgg
     2461 caggaggttc gcgcgtgact acctgatcga tctgctgtgg cccgacgtac cgaccgaacg
     2521 cggccgccat tcgttgtcgc aggcggttac ggtgctgcgc cagacggtgg ggccacagaa
     2581 tctggccgca catcggacga gcatcgcgct ggccgacgga gtcgtggaaa ccgacgcgct
     2641 gcagctcgag gcgggcgaac cggaaatccg cggccggttc ctcgagggat tcgaggtgcg
     2701 gggtgcgcgg ggattcgacg aatggcgcga ggggtgggtc gcccagctgc atccccgcat
     2761 ccgtgactgc ctggtgaagc gcatggatgc ggcacgtcgc atcggcgact tcgccacggt
     2821 ggagcggcac gcccgggtgc tcgaggaagt cgatccggtc tccgaagatg ccgtccgcgg
     2881 cgtcatggag gcacgggcct gggtggggga tcgcagcagc gccctcaagg cgttcgcgcg
     2941 cttcgccgcc cggctcgccg aggacctcaa ggccaagccg agcgcggagc tccaacgcat
     3001 cgccgatctg ctgcgggaag gccgccgggc cgctccacgg ccgacggccg acggcgtccc
     3061 ggcccgccag gagcggcgct tcgaggcgga aacgatcctc ggacgacagc gcgagttctc
     3121 gacgctgtac gacgcctggc tcgaggtacg gcagagccgg tcccgggtga tcgtgatcct
     3181 cggtgagccg ggaattggca agaccaccct caccaacgcg ttcatctcgt cgtgccagat
     3241 ggagggcggc atcatcgcgc gcacccaggc ctacgacgcg gaacgcgagc tgccgtatgc
     3301 cgtcctggcg gagctggtgc gccagctcac cctgcaacgg gcgatcggcg cggcggaacc
     3361 ggaagcgctg gcggagctga cgcggttgac gcccgaggtc ctctccgctt ttccgggcgt
     3421 gccgcggccc gacgagtggc cgcccgaggt cgtcccgctg cgattggcgg acgcgttctt
     3481 caaggcggtg atggccgcga ccgacgagtt cccggtcgtg ctgatcgtgg acgacatcca
     3541 cgcggcggac aacgcgagcg tcgcgatcct gcacatgctg gcgcgcaaac tcgcgggtct
     3601 gcgggtgctc ctgattgtga cggggcgccc cgggagcctg cggccgccat ccagcgccca
     3661 tgcgctggtc accgactcgg tgatcgaagg actgggaacc gtggagctcg acgccttgtc
     3721 gaacgaggcg gcggcgctgc tggtcgcgcg cgcgagcgcc aaagggatcg agcgctgggg
     3781 cgaaccgccc atcgatcgga tcgtccgggc gggcggcggg aatccgctgg cgctcgagct
     3841 gttgagccgg gactgggtgg agaatggcgc cgactccctc gtggggaagc tcgatccggt
     3901 gcatcccctg ccggccggat tgcagagcat tcccaaggcg atccggacga tggtcgagcg
     3961 gcagaccgag gcgctggccg agcgcacccg ggcggtgttc gacctcgcgt cggtgctggg
     4021 gcgccgcatg ggagatctgt ggttgtatca agtgatcggc tgctcggaga ccgaggcgct
     4081 gtaccaactg ggccagcttc tcgaggcccg gctgctgcgg gacattgcgg gcacgcttga
     4141 attccgcaac gagctgattc gtgctcacgc ctactacgag attccaagca cggttcgact
     4201 ggagttgcac cgcagaatcg ccggcgtgct cgaagcgcaa gggggaaacg atgcaaatca
     4261 cctcgaaatc gcctggcact acatcctcgg ccgtgatctc gagcgggcta ttcccgttgc
     4321 tctttctggg gcagagcaga gtcttcgcgc gggggcgccc cgagaagctg aactgatcct
     4381 tcgtcgtctc atgcgagaga aggtcgatgc gcgcagggcg gggcggatgg agctgttcct
     4441 cagcgaggcg ctgctaagtc agtcgaaggc tgatgaagcg ctctcgattc tcgatcagcg
     4501 actgggagat cagactctta ctaaaccgga agtggctcag gccgcccgtc tatacgcatc
     4561 tggagtctac ctcgttaaca agaactcggc ggccagacac agcgagatgg ccaaacgagc
     4621 cctgcaagtg gctcacgagt gcggagaccc agatcttctt gcatacgccc tcttcgaatc
     4681 ggggagggcc ggtgtggagt ctggagacca ggggttgact agaactacgc gccaagccgt
     4741 cagggcaatc atcgcggagg aagacggcaa cgcgccatcg atag
//
LOCUS       DBNGZW010000604         7254 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_724949, whole genome shotgun sequence.
ACCESSION   DBNGZW010000604 DBNGZW010000000
VERSION     DBNGZW010000604.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_724949"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1501
                     /locus_tag="AC16JC_14255"
     CDS             <1..1501
                     /locus_tag="AC16JC_14255"
                     /inference="COORDINATES: protein motif:HMM:NF024883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HIR5165909.1"
                     /translation="DPEQLLDDILTQARRLTQSDAGSLYLIETDVFGAKGLRFRLAQN
                     LTLPNVPLVQFTMPLDRTSLAGYAAVTGEPLVIDDAYLLPPDVEYTINRSFDERHGYR
                     SRSMLIIPMKDHKGEVIGVVQLINRKRDPNALLSTMADVEREVVSYSKRTVELVTALA
                     GQAAVSIENSRLYEQIEKMFEGFVNAAVQAIEQRDPVTQGHSRRVADTTVTLARVVDR
                     ADQGAYRHIRFTREQINEIKYAGLLHDFGKVGVREHVLTKAKKLYPGQLELIRQRHAF
                     VQRTLERDFWRQRAEFLERQGADGYEGFVSRLDEEHRRELQQLDRFLAVVLQANEPSL
                     LASESEADLAELGRRTYQDLSGEQKPLLTEQEMRYLTIRKGSLDDQEREQIESHVTQT
                     WVFLQEIPWTKELQEIPVIAYGHHELMNGTGYPRRIAGPEIKIQTRMMTISDIYDALT
                     AQDRPYKKAVPTPKALDIMDRELVKSGQLDAELFQLFVEAKVYLEATPD"
     gene            complement(1502..2347)
                     /gene="ypfJ"
                     /locus_tag="AC16JC_14260"
     CDS             complement(1502..2347)
                     /gene="ypfJ"
                     /locus_tag="AC16JC_14260"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013580740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_02809 family neutral zinc metallopeptidase"
                     /protein_id="HIR5165910.1"
                     /translation="MKWTPGGTSQNVEDRRGQTGGGGGYGGGGLRLGLGGALVLLVLS
                     LIFRQNLFQFFETPGGAVSTGAPQASSPQEDTLVQFVSFVLDSVQASWARTLPAVGVP
                     YQDAHLVLFRDYSESACGFAETATGPFYCPGDQKVYIDLGFYQELRDRFGAAGDFAQA
                     YVLAHEIGHHVQALMGTEARVRRRGQDQSVRLELQADCYAGIWAHVADQEHLLDAGDV
                     EEGINAAAAVGDDRLQRMATGHVNPETFTHGSSAERAAWFRRGYTTGRLDACDTFSGS
                     VDIGN"
     gene            complement(2379..3035)
                     /locus_tag="AC16JC_14265"
     CDS             complement(2379..3035)
                     /locus_tag="AC16JC_14265"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR5165911.1"
                     /translation="MSAAVFETIRVRAGCAPLLRRHEARLAAACGVLGLPQPEPSLAD
                     AVARQAGMRDGVVRVEVSARGTTVTRRELPSLLPLTVRVASTPHTPYPCKVTSRNTFE
                     AALAEARAAGGEADDALLITPGGLVAEGTAWNIFWWEADGLATPPLSLGVLPGVARGR
                     VMELVPTVERARAPGELVGRGLFATNAIRGVVAIVRLNGAPVPHDARTTQLADRFWPE
                     "
     gene            complement(3032..4249)
                     /locus_tag="AC16JC_14270"
     CDS             complement(3032..4249)
                     /locus_tag="AC16JC_14270"
                     /inference="COORDINATES: protein motif:HMM:NF012640.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HIR5165912.1"
                     /translation="MALAATLDPPFALLEDGRGTGLTHLVWGTSRRWRRRLGECGDLT
                     PPPTEGMPWYGMLAYEAGLPEWVSPSQAALPQPLADWFVPANRILLGSDGRALRLDGP
                     EAEARWREAPDSGLPVPPPTVLTARAWRSFDAPAFEAAVRRAHAHIAAGDIYQVNLAL
                     AEHWEYPGTAWDAYRRLREVNPSPWMGFADFGDWQLVCGSPELLIEVTGGPSGRRVRT
                     RPIAGTRKKNGNAEADARMRDELRLDEKERAEHLMLVDLARNDLGRVSAFGSVQVAEL
                     EVVEEYSHVFHLVSEVRGRLRPEAGVAEVIGSVFPGGTITGCPKLRAMEIIRELEPVG
                     RGAYTGGFGWIGPPGLQLNIVIRSAVVTGGRVLIQAGAGIVADSIAEREWRESLRKAE
                     AVRLAFGASPAGA"
     gene            complement(4296..4937)
                     /locus_tag="AC16JC_14275"
     CDS             complement(4296..4937)
                     /locus_tag="AC16JC_14275"
                     /inference="COORDINATES: protein motif:HMM:NF024206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR5165913.1"
                     /translation="MTNAQQGSLTASDFFAAVRAGSVERVRELVTQTPAFAHARSEGA
                     TALHVAAIAGNRAMVDLLLSARADVNARDDTYQMTPIAWANEEGHREMVRHLYDRGAE
                     VDLHQAAAYGLVDHVKDLVRSSRRQVNAVVGGWTPLQLATIWGHPEVVELLLSRGADP
                     LVRDPYGRTTVEIARAQVATNAQSTPIVREERRREIVEACARIAQLLEQRGSR"
     gene            complement(4995..5351)
                     /locus_tag="AC16JC_14280"
     CDS             complement(4995..5351)
                     /locus_tag="AC16JC_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012506331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5165914.1"
                     /translation="MNYTLVGLLGLAAGVVSGLFGVGGAIVIVPGLVLLGKIPQHTAN
                     GTSLGALLLPVGLLGFLQYYKRGQVNIPYAALIAVGLFVGALLGAKLAGSLSDVWLKR
                     AFGVLLLFVAGKLLLS"
     gene            complement(5348..6877)
                     /locus_tag="AC16JC_14285"
     CDS             complement(5348..6877)
                     /locus_tag="AC16JC_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR5165915.1"
                     /translation="MTLDPITREVMAHAFAGVAEEMGLVLIHSAVSPNIRERRDASAA
                     LFDAAGDMIAQAAHIPVHRGALADAVAAVRAEKPQPGDLYIVNDPYTGGSHLPDLTLV
                     GVIRTTEGIGGYSVVRAHHSDVGGATPGSMPAGARELLAEGLVLPPVLYTPEVERVLL
                     ANVRTPDVRRADLAAQRAAVERGAEGLWALAARHGWAFVQDAARELQNDAEARTRAVL
                     GRLPRLTAAATDWLEGDGVTDGDIPIRVTVDIRDRTFHADFTGTAPAVPGNVNCPLAV
                     TRAAVLFVVRTLLGEDTPMNAGVARAVRVSAPVGCMVNARWPSAVAAGNVETSQRVAD
                     VVFRALAVAGLSVPAQGQGTMNNITLGGSGWTYYETLGGGQGAGPSGPGPSAVHAGMS
                     NTRNTPVEVLELEYPVRVRRYALRPGSGGAGRWRGGDGVVREIELLAPAEASILSERR
                     QHGPSGAAGGAGGACGRNLLNDAPLPAKANLRLEAGDVLRIETPGGGGWGESPNPRSD
                     S"
     gene            complement(6874..>7254)
                     /locus_tag="AC16JC_14290"
     CDS             complement(6874..>7254)
                     /locus_tag="AC16JC_14290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165916.1"
                     /translation="EITVPLTDGEPDSIEAAFHAAHRARYGHLGAAGAVEIVNVRVVA
                     ERTGGRPTWGRRAGIGRPTPESRRIVVGGKAIRAQVWPLGELPPRLRIPGPAILAGPD
                     ATGLVEPGWRGTVHETGAVLLERI"
BASE COUNT         1145 a         2663 c         2436 g         1010 t
ORIGIN      
        1 cgacccggag cagctgctcg acgacatcct gacgcaggcg cggcggctca cgcaatcgga
       61 tgccggcagc ctctatctga tcgagaccga cgtcttcggc gcgaaaggac tgcggttccg
      121 gctggcgcag aacctcaccc tgccgaatgt gccgctggtg cagttcacga tgccgctcga
      181 ccgcacgagc ctcgcgggct acgcggccgt gaccggcgag ccgctggtga tcgacgatgc
      241 gtatctgctc ccgcccgacg tcgagtacac catcaaccgc tcgttcgacg agcggcacgg
      301 ctaccgctcg cggtcgatgc tcatcatccc gatgaaggac cacaagggcg aagtcatcgg
      361 ggtggtgcag ctgatcaacc gtaagcgcga ccccaacgcc ctcctttcca ccatggccga
      421 cgtcgagcgc gaggtcgtct cgtactccaa gcgaacggtc gagctggtga ccgcgctcgc
      481 aggccaggcg gcggtatcga tcgagaacag ccgcctgtac gagcagatcg agaagatgtt
      541 cgaggggttc gtcaacgcgg ccgtacaggc gatcgagcaa cgcgacccgg tcacccaggg
      601 tcacagcagg cgcgtcgcgg acaccacggt caccctcgcc agggtcgtcg atcgggccga
      661 ccagggcgcc tatcgccaca ttcggttcac ccgcgagcag atcaacgaaa tcaagtacgc
      721 cggactgctc cacgacttcg gcaaggtcgg ggtgcgggag cacgtcctga ccaaggccaa
      781 gaagctctat cccggccagc tggagctgat caggcagcgc cacgcgttcg tgcagcgcac
      841 cctggagcgg gacttctggc gccagcgtgc cgagtttctc gagcggcagg gtgccgacgg
      901 ctatgagggg ttcgtgtcgc ggctggacga ggagcatcgc cgggagctgc agcagctcga
      961 tcgattcctg gcggtggtgc tgcaggcgaa cgagccgagc ctgctcgctt cggaaagcga
     1021 agccgacctg gccgagctcg gccggcgcac ctaccaggac ctctccgggg agcagaagcc
     1081 gctgctcacc gagcaggaga tgcggtacct cacgatccgc aagggcagcc tggacgacca
     1141 ggagcgggag cagatcgaga gccacgtcac gcaaacctgg gtgttcctgc aggagattcc
     1201 ctggaccaag gagctgcagg agatcccggt gatcgcctac gggcatcacg agctgatgaa
     1261 cgggacgggc tacccgcgcc ggatcgccgg cccggagatc aagattcaga cgcggatgat
     1321 gacgatcagc gacatctacg atgcgctcac ggcccaggac cggccgtaca agaaggcggt
     1381 tcccacgccc aaggcgctgg acatcatgga ccgcgagctc gtgaagtccg ggcagctgga
     1441 cgcggagttg ttccagctgt tcgtcgaggc gaaagtgtac ctcgaggcga cgccggacta
     1501 gtcagttccc gatatccacc gagccgctga acgtatcaca ggcgtcgagg cggccggtcg
     1561 tgtagccgcg ccggaaccac gccgcccgct ccgccgatga gccgtgggtg aaggtttccg
     1621 gattgacgtg ccccgtcgcc atgcgctgca gccggtcgtc tcccaccgcg gcggcggcgt
     1681 tgatgccctc ctccacgtca ccggcgtcga gtagatgctc ctggtcggcc acgtgcgccc
     1741 agatgccggc gtagcagtcg gcctggagct ccaggcggac cgattggtcc tgcccccgac
     1801 ggcgcacgcg cgcctccgtc cccatcagcg cctggacatg gtgcccgatc tcgtgggcga
     1861 gcacgtacgc ctgggcgaag tcgccggcgg cgccgaaccg gtcgcgcagc tcctggtaga
     1921 agcccaggtc gatgtacacc ttctgatccc ccggacaata gaacggtccc gtggccgttt
     1981 cggcaaagcc gcacgccgac tcgctgtagt cgcggaacag cacgaggtgc gcgtcctgat
     2041 acggcacgcc cacggccggc agcgttcgcg cccaggatgc ctggacgctg tcgagcacga
     2101 acgagacgaa ctgcaccagc gtgtcctcct gaggcgagga ggcctggggc gcgccggtcg
     2161 agacggcgcc cccgggggtt tcgaagaact ggaacaggtt ctggcggaag atcaggctca
     2221 ggacgagcag cacgagcgcc ccgcccaggc cgagtcgcag cccgccgccc ccatagccac
     2281 cgccgccacc ggtctgcccg cgtcggtcct cgacgttctg actggtgcct ccgggcgtcc
     2341 acttcatgag gtccctcccc tccgggtcga ctcgccggtc attccggcca gaatcgatcg
     2401 gccagctgcg tagtgcgcgc atcgtgcggc acgggagcgc cgttcaggcg cacgatcgcc
     2461 acgactcccc gaatcgcgtt cgtggcgaac agcccgcgtc cgacgagctc gccgggcgcg
     2521 cgcgcgcgct ccaccgtcgg cacgagctcc atgacgcggc cgcgcgccac cccggggagc
     2581 acgcccaggg acagcggcgg cgtcgccaga ccatccgcct cccaccagaa gatattccac
     2641 gccgtgcctt cggcgaccag cccgcccggc gtgatcagca gggcgtcgtc ggcctctccc
     2701 ccggcggccc gcgcctccgc cagcgccgct tcgaacgtgt tacgggaggt gactttgcag
     2761 ggatagggcg tatggggtgt ggacgcgacg cggacggtga gcgggaggag cgacggcagt
     2821 tcgcgcctgg tcaccgtggt cccgcgcgca ctcacttcga ctcgcaccac gccgtcccgc
     2881 atgccggcct ggcgcgccac cgcgtcggcc agcgagggct ccggctgcgg cagcccgagg
     2941 acaccacacg ccgcggcgag ccgggcctcg tgacgccgca gcagcggggc gcaccccgcc
     3001 cggacccgga tcgtctcgaa gacggccgcg ctcatgcgcc ggcgggcgat gcaccgaacg
     3061 cgagccgcac cgcttccgcc ttgcgcaggg attcccgcca ttcgcgctcc gcgatcgagt
     3121 cggcgacgat ccccgcaccg gcctggatca gcacgcgccc gccggtgacc acggcgctgc
     3181 gtatgacgat gttgagttgc agcccggggg ggccgatcca gccgaagccc ccggtgtagg
     3241 cgcctcggcc caccggctcg agctcgcgga tgatctccat cgccctgagc ttgggacacc
     3301 cggtgatcgt gccgccgggg aacacactcc cgatcacctc cgccaccccc gcctcggggc
     3361 gcaaccggcc gcgcacctcc gacacgaggt ggaagacgtg cgagtactcc tcgacgacct
     3421 ccagctcggc gacctgcacc gagccgaagg ccgacactcg gccgaggtca ttgcgcgcca
     3481 ggtcgacgag catcagatgc tcggcgcgct ccttctcgtc gagccgcagc tcgtcgcgca
     3541 tgcgcgcgtc ggcttccgcg ttgccgttct tcttgcgcgt gccggcaatc gggcgggtgc
     3601 gcacccggcg cccggacggc ccccccgtga cctcgatcag cagctcgggc gagccgcaga
     3661 ccagttgcca gtcgccgaaa tccgcgaacc ccatccacgg tgacggattg acttcgcgca
     3721 gccgccggta ggcgtcccac gccgttcctg gatactccca gtgctcggcc agggcgaggt
     3781 tgacctggta gatgtcaccg gcggcgatgt gcgcgtgggc gcgtcgcacg gccgcctcga
     3841 atgccggcgc atcgaacgag cgccaggccc gtgcggtgag cacggtgggc ggaggaacgg
     3901 gaaggccgga atctggagcc tcgcgccacc gcgcttccgc ctccggacca tcgagtcgga
     3961 gggcgcggcc gtcgctcccc agcaggatcc ggttggcggg cacgaaccag tcggcgagcg
     4021 gctgtgggag cgcagcctgt gacggcgata cccattcggg aaggcccgct tcgtacgcca
     4081 acatgccgta ccagggcatc ccctcggtcg gcggtggcgt gagatcgccg cactccccca
     4141 gccgacggcg ccagcggcgg ctggtccccc aaacgaggtg cgtgaggccc gtcccgcgac
     4201 catcctccag cagtgcgaac ggcgggtcga gcgtcgcggc gagcgccacc aggcggtcga
     4261 cggcggcggc cggccgggcg tgaagggtcg tcaggtcagc gtgaaccgcg ctgctcgagc
     4321 agctgcgcga tcctcgcgca cgcctcgacg atctcacggc ggcgctcctc gcgcacgatc
     4381 ggcgtgctct gggcgttggt ggcgacctgc gcccgcgcga tctcgacggt ggtccgaccg
     4441 tagggatcgc gcaccagggg atcggcgccg cgggacagga gcagctccac gacctcgggg
     4501 tgcccccaga tcgtggcgag ctgcagcggc gtccacccgc ccaccactgc gttcacctga
     4561 cgtcgcgagc tgcggaccag gtctttcacg tggtccacca ggccgtaggc ggccgcctgg
     4621 tgcaggtcca cctcggcgcc gcgatcgtag aggtggcgca ccatctcgcg gtgtccttcc
     4681 tcgttggccc aggcgatcgg cgtcatctga taggtgtcgt cccgggcgtt cacgtcggcc
     4741 cgggcgctga ggagaagatc caccatggcg cggttgccgg cgatggccgc gacatgcagg
     4801 gccgtggccc cctcgctgcg cgcgtgggcg aaggccggcg tctgcgtgac gagctcacgc
     4861 acccgttcga ccgagcctgc tcgaacggcc gcgaagaagt cagaagcggt gagggaaccc
     4921 tgctgcgcgt ttgtcatgca tgctccgtgg cgacccatga aagctgtgtc ggtggtcgtg
     4981 cagaggcaac gccctcacga gagcagcagc ttccccgcga cgaagagcag gaggacgccg
     5041 aacgcccgct tcaaccacac gtccgacaac gatcccgcga gtttcgcgcc cagcagggca
     5101 ccgacgaaca ggcccacggc gatgagggcg gcatacggaa tgttcacctg cccccgcttg
     5161 tagtactgca gaaaaccgag caaccccacc ggcagcagga gcgctccgag cgacgtgccg
     5221 ttggcggtgt gctgggggat cttcccgagc agcacgagac ccggcacgat gacgatcgcg
     5281 ccccccaccc cgaacaatcc ggataccact ccggccgcga gccccagcag ccccaccagt
     5341 gtgtagttca tgaatcactc cttggattcg gagactctcc ccatcctcca ccacctggcg
     5401 tctcgatgcg caacacatcc ccggcctcga gccgcagatt cgccttggcc ggcagcgggg
     5461 cgtcattcag caggttccgc ccgcacgctc caccggcgcc gcccgcggct ccactcgggc
     5521 cgtgctgccg ccgctcgctc agaatgctcg cctccgccgg agcgagcagc tcgatttcgc
     5581 gcacgacccc gtctccgcca cgccaccggc cggcgccgcc actgcccggg cgcagggcat
     5641 agcgccggac gcgcaccggg tattccagct ccagcacttc gaccggggtg tttcgcgtgt
     5701 tcgacatccc ggcgtgcacc gcggacgggc cggggcccga cggcccggct ccctgaccgc
     5761 cgcccagtgt ctcgtaatac gtccagcccg agccgcccaa ggtgatgttg ttcatcgtgc
     5821 cctggccctg cgccgggacg gacagtcccg cgacggcgag cgcccggaac accacatcgg
     5881 cgacgcgctg cgacgtctcg acgttccccg ccgccaccgc gctcggccac cgggcattga
     5941 ccatgcaccc cacgggagcg ctgacccgga ccgcacgggc gacgccggcg ttcatgggcg
     6001 tatcctcgcc cagcagcgtg cgcacgacga acagcacggc ggcgcgagtg accgccagcg
     6061 ggcagttgac gttgcccgga accgccgggg ccgtgccggt gaagtccgcg tggaaggtac
     6121 ggtctctgat gtcgacagtg acgcggatcg ggatgtcgcc gtcggtgacg ccgtccccct
     6181 cgagccaatc cgtcgccgcc gccgtgagac gcgggagacg gccgagtacc gcacgggtgc
     6241 gcgcttcggc gtcattctgc agctcgcggg ccgcgtcctg gacgaacgcc cagccgtgcc
     6301 gcgcggcgag tgcccacaac ccctccgcgc cccgctccac cgcggcgcgt tgcgccgcca
     6361 gatccgcacg acgcacatcc ggtgtccgca cgttcgcgag caggacccgc tcgacttccg
     6421 gcgtatagag cacgggcggc agcaccagcc cttcggccag cagctcacgc gcccccgccg
     6481 gcatgctgcc cggggtcgca ccgcccacgt ccgagtggtg cgcccggacc acgctgtagc
     6541 cgccgatgcc ctccgtcgtg cggatcaccc cgacaagcgt gaggtcggga aggtgcgatc
     6601 cgcccgtgta cggatcgttg acgatgtaga gatcgcccgg ctgcggtttc tcggcgcgca
     6661 ccgccgcgac cgcgtccgcc agcgctcccc gatggacggg aatgtgggcg gcctgcgcga
     6721 tcatgtcccc cgcggcatcg aacagcgccg ccgaggcatc ccgccgttcc cgaatattgg
     6781 gggacaccgc cgagtggatc aggaccaacc ccatctcctc ggcgacgccg gcgaaggcgt
     6841 gggccatcac ctcgcgggtg atgggatcga gcgtcatatg cgctccagta gcacggcgcc
     6901 tgtctcgtgc acggtcccgc gccagcccgg ctcgaccagg ccggtcgcgt cgggccccgc
     6961 gaggatcgcg ggcccgggaa ttcgcagccg agggggcagc tcccccagcg gccagacctg
     7021 cgcccggatc gccttgcctc cgaccacgat gcgccgcgac tcgggagtcg gccgcccgat
     7081 ccccgctcga cgcccccagg tgggccggcc acccgtccgc tccgccacga cccgaacgtt
     7141 gacgatctcg accgctcccg cagccccgag gtgaccgtag cgcgcccggt gcgcggcatg
     7201 gaacgccgct tcgatgctgt cgggctcgcc gtccgtcaac ggcacggtga tctc
//
LOCUS       DBNGZW010000605         3099 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1064976, whole genome shotgun sequence.
ACCESSION   DBNGZW010000605 DBNGZW010000000
VERSION     DBNGZW010000605.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3099)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3099)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3099
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1064976"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..2638)
                     /locus_tag="AC16JC_14295"
     CDS             complement(23..2638)
                     /locus_tag="AC16JC_14295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002617405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR5165917.1"
                     /translation="MATLVPRALLSAALLMELTSGCAHPRTAGPEQIADTTAHPPQPN
                     SSTVSWTEIQRNTTDPIEMALVGRVSGVEVSRTSDGGIAVRIHGPSSFYSGEAPLYVL
                     DGVPIQPGLNGALTGISPYDIESIEVLKDPASTAIYGVRGGNGVIVITTKWSDCRDRT
                     PPCPPRRTAPRPAPRAVPAPADRIDSLLARMTLEEKLGQLNLLPAGGRATPQQAEEVR
                     RGTVGGFLNVDGAEAARDIQRVAVEQSRLRIPLLLGLDVIHGYRTIFPIPLAEASTWD
                     PDVVEATARAAGREARAAGINWTFAPMVDIARDARWGRIAEGSGEDPYLGSALAAARV
                     RGFQQNILATAKHFAAYGGAEAGREYNTVDLSERTVRDIYLPPFKAAVDAGVGSIMSA
                     FNEVGGVPSSASPWLLDTVLHREWKFDGFVVSDWNSIDELRSHGIAGSRAEAGRLALV
                     AGVDMDMVSRIYLDSLPALVTSGRLPLARVDAAVRRLLHAKQKLGLFDDPYRPAPAQP
                     LDRALARRVAQEAIVLLKNEGGILPLGTGAKTIAVIGPLAADTVDPLGPWHAQGRPGD
                     VVSVLAGIRERAGPGATVLHAAGTGVTDGDTSGIAAAVDLARRADVTVLVVGEADTMS
                     GEAGSRTRLDLPGAQDRLQQAVVATGRPVVLVLMNGRPLVLSWAAEHVPAILDTWFLG
                     VETGHAVADVLFGDVNPSGRLPVTFPRTVGQLPIYYGHKNTGRPAAAEHYTSKYLDVP
                     VTPLYPFGYGLSYTTFTYRDLRLAASRLGPRDTIAVSVTVANTGARRGTEVAQLYVRD
                     EVASVTRPVRELKGFQRVTLDPGEARTVQFRVPVRDLAFWNASMKLVVEPGTFRVFVG
                     PNAAEGLESGFEVTP"
     gene            complement(2707..>3099)
                     /locus_tag="AC16JC_14300"
     CDS             complement(2707..>3099)
                     /locus_tag="AC16JC_14300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165918.1"
                     /translation="AVADYSGNEVVGVPGLTYAASFVWAPAPLAPLQLRFGVQGASSY
                     FTDDANQVEVPGSRIASLTAATAEPVALAPGLGLHGFVTLNNLFDHAYIASAFLNPDV
                     VAGEPVAFEPGLPRNLVVGLSLVRRRGR"
BASE COUNT          447 a         1083 c         1135 g          434 t
ORIGIN      
        1 cgccaggtga ggccccggtc cgtcaaggcg taacttcgaa gccgctctcc agcccttccg
       61 ccgcgttggg tccgacgaac acccggaacg tgcccggctc gacgacgagc ttcatggacg
      121 cgttccagaa tgccaggtcc cgcaccggga cgcggaactg cacggtgcgc gcctcgcccg
      181 ggtcgagcgt gacgcgctgg aaccccttca actcgcgtac gggacgggtc acactggcca
      241 cctcgtcacg cacgtagagc tgcgccactt cggtcccccg gcgggcgccg gtgttcgcga
      301 ccgtcaccga caccgcaatc gtgtcgcgcg gtccgaggcg cgacgcggcg agccgcaggt
      361 cgcggtaggt gaaggtggtg tagctcaggc cgtagccgaa cgggtacagc ggtgtcacgg
      421 ggacgtcgag gtacttggag gtgtagtgct ccgcggccgc cgggcggccg gtgtttttgt
      481 ggccgtagta aatcgggagc tgccccacgg tccgcgggaa cgtcaccggc agccggccgc
      541 tcgggttcac gtcgccgaac aggacgtcgg cgaccgcgtg gccggtttcg acgccgagaa
      601 accacgtgtc gagaatcgcg ggaacgtgct cggcggccca tgacaggacg agcggcctgc
      661 cgttcatgag gaccagcacg acggggcggc ccgtggccac cacggcctgc tgcagccgat
      721 cctgcgcgcc ggggagatcg agccgggtgc ggctgcccgc ttcgccgctc atcgtgtccg
      781 cctcccccac gacgaggacg gtcacgtccg cccgccgggc gagatccacg gcggcggcga
      841 tgccgctcgt gtcgccgtcg gtgacgccgg tgcccgcagc gtgcagcacg gtggctcccg
      901 gacccgcccg ctcgcggatg ccggcgagca ccgacacgac gtcgccgggc cgtccctgcg
      961 cgtgccaggg gccgagcggg tcgaccgtgt cggcggcgag cgggccgatg acggcgatcg
     1021 tcttcgctcc ggtcccgagc ggcaggatcc ccccctcgtt cttgagcagg acgatcgcct
     1081 cctgggcgac gcgtcgggcg agggcgcgat cgagcggctg tgccggtgcg ggacgatagg
     1141 ggtcgtcgaa caggcccagt ttctgctttg cgtgcagcag ccggcgcacc gccgcatcca
     1201 cccgggcgag cggcagccgt ccggaagtca ccagcgcggg gagactgtcc aggtagatcc
     1261 ggctcaccat atccatgtcc acgccggcca cgagcgccag ccgtccggcc tcggcccgcg
     1321 acccggcgat cccgtgcgag cgcagctcgt cgatcgagtt ccagtcgctc accacgaagc
     1381 cgtcgaattt ccactcgcgg tgcaacacgg tgtcgagcag ccaggggctc gcggagctgg
     1441 gcaccccgcc gacctcgttg aacgccgaca tgatggaccc gacgccggcg tccacggccg
     1501 ccttgaaggg gggcaggtag atgtcgcgca ccgtgcgctc ggacaggtcc accgtgttgt
     1561 actcccggcc cgcctcggcc cccccgtagg cggcgaagtg tttcgccgtg gcgaggatgt
     1621 tctgctggaa gccgcgcacg cgggccgccg ccagcgccga gccgaggtag gggtcctccc
     1681 ccgagccctc ggcgatccgg ccccaccgcg cgtcgcgggc gatgtccacc atgggcgcga
     1741 aggtccagtt gatccccgcc gcccgtgcct cgcggccagc ggctcgcgcc gtcgcctcca
     1801 cgacatcggg gtcccaggtg ctcgcttcgg ccagcgggat ggggaagatc gtgcggtagc
     1861 cgtgaatgac atcgagcccg aggaggagcg ggatgcggag gcgcgactgc tcgaccgcca
     1921 cccgctggat gtcgcgcgcg gcttccgcgc cgtcgacatt caggaacccc ccgaccgtgc
     1981 cgcggcgtac ctcctccgcc tgctgcggtg tcgcgcggcc gccggcgggg agcaggttca
     2041 gctggccgag cttctcctcg agcgtcatgc gcgcgagaag ggaatcgatg cggtcggcgg
     2101 gcgcgggcac ggcgcgtggc gcgggccgcg gtgcggtgcg ccgtgggggg cagggcggcg
     2161 tccggtcgcg acaatccgac cacttcgtcg tgatgacgat gaccccattg ccgccgcgta
     2221 ccccgtaaat cgccgtgctc gccggatcct tgaggacctc gatggactcg atgtcgtacg
     2281 ggctgatgcc ggtgagggcc ccgttcagtc ctggctggat cggcaccccg tccaggacgt
     2341 acagcggcgc ctcgccgctg tagaacgagc tcgggccgtg aatgcggacc gcgataccgc
     2401 cgtccgaggt gcgcgatacc tcgacgcccg agacgcgacc gaccaacgcc atttcgatcg
     2461 ggtcggtggt attgcgctga atctccgtcc aactgacggt cgaagaattg ggctggggcg
     2521 gatgggcagt cgtatccgcg atctgctccg ggccggcggt gcgcgggtgg gcacatccgg
     2581 acgtgagctc catcagcaga gcggcgctca gcagggcacg cggaacgagc gtggccatgc
     2641 ggccaatata cgccgcggcc gtgcactgac tagggcccgc gccacggtgg tgtagcgagc
     2701 gccgggtcac cgcccgcgcc gccgcaccag cgacagtccg acgaccaggt tgcgcggcag
     2761 tcccggctcg aacgccaccg gctctccggc gaccacgtcc ggattgagga acgccgaggc
     2821 gatgtaggcg tgatcaaaga ggttgttgag cgtgacgaag ccgtgcagcc cgaggccggg
     2881 ggcgagggcg acgggctcgg cggtcgcggc ggtgaggctg gcgatgcggg agcccggcac
     2941 ctccacctga ttggcgtcgt cggtgaagta ggaggaggcg ccctggacgc cgaagcgcag
     3001 ctgcagtggc gcgaggggcg ccggcgccca gacgaagctc gccgcgtacg tcaggccggg
     3061 cacgcccacg acctcgttcc cgctgtagtc ggcgacggc
//
LOCUS       DBNGZW010000606         3424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1815094, whole genome shotgun sequence.
ACCESSION   DBNGZW010000606 DBNGZW010000000
VERSION     DBNGZW010000606.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1815094"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..313
                     /locus_tag="AC16JC_14305"
     CDS             <1..313
                     /locus_tag="AC16JC_14305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165919.1"
                     /translation="EEDRNLGKYMAQEWSRGRFDPADEVCRRWDELGKQVIVLLQSSG
                     ELRADVDPALIHRILGDVYHDTIVMWLESPEPPFSLQDELRRRLTLVVEGVGRKPKEG
                     R"
     gene            310..1704
                     /locus_tag="AC16JC_14310"
     CDS             310..1704
                     /locus_tag="AC16JC_14310"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR5165920.1"
                     /translation="MRRLLFLLLAAGPAAAQAPTDTLHLTLDQAVQRALDRSFAMRLA
                     RADVLDADGRVREAFSAALPQVTGTLTYTRQFASIFQGLGGTDTSSFGDLFKNTPFGA
                     ANAWNFQVQATQLLWSGGKVGSGLSAAKAARSIATLAASETTAEVSFQVKQAYWNAAL
                     QSRLLEIATGNLDQARAQLRQVQLFRQAGTRAEYDLLRAQVDAANQEPAVVAARNDLD
                     LAVLELKRLLDLPADQPLELATPLESPEATIPVVANDSIGGPDRPALAQAEAAVLQQK
                     EVLDVARADRWPTLSVATTYNQQAFPQGVFPSSVNQFRRGWNGEVKLSFPIFLGFRTG
                     ARVEQARAALQRAEAQRDQVHKQVELDVARARAEVARAQALLVARRETVRQADRALHI
                     AGVRYTNGIATQLEVSDARVAAQQSQVNEVQATRDYVVALARLERALGRAVPVVQQPI
                     TPIAQSLNSEETRP"
     gene            1701..2771
                     /locus_tag="AC16JC_14315"
     CDS             1701..2771
                     /locus_tag="AC16JC_14315"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5165921.1"
                     /translation="MKRYTMLGALAALVLTGAAAAGCGKGEAANNPADSVRTAVLGPD
                     DVARAARRDLIAGVPVSGTLQPSVEVRIASPIPEVVEQVFVKEGQAVQRGQVLARFRT
                     SAVEPAALSAEAQRRKAEADYARMQNLYKEGAVSEQDVESAEVALRAAQANEAGARKR
                     LDEATVRAPASGVISKRAVDSGDRVKDGDLLFQLVDTRELEFEATVPSEYVSAVRIGA
                     PVMLTVTGADSGITGRVARVNATVDEATRQVKVYLTVANRGGRLVGGLFATGRIVLQD
                     VKNAVTVPRTAVHSGPDGKAYVLIVEGGKIVRRDVTMGAADEQASLVEIKTGLQGGET
                     VIVGPANGLEAGDPATIAGGEG"
     gene            2775..>3424
                     /locus_tag="AC16JC_14320"
     CDS             2775..>3424
                     /locus_tag="AC16JC_14320"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5165922.1"
                     /translation="MFLSDLSIKRPVFASMMMLALVVLGLFSYRRLNIDQFPDVEFPF
                     LVIQTRYAGASPESVEREVTKKIEESVNTVEGVKRIQSTSTEGFSTIFVLFNLGTKLM
                     DAQADVRAKIDAIRQDLPTDIDLPVISRANPSDQPIFVLAVQGESWALRDLTRLADEV
                     VSRRIENVSGVGSVSLVGGLKREIHVLLQPDRLQALGISPDLVTAALRRENGDVPAG"
BASE COUNT          540 a         1136 c         1267 g          481 t
ORIGIN      
        1 cgaagaagac cggaacctgg ggaagtacat ggcccaggaa tggagccggg gccgcttcga
       61 tcccgccgac gaggtgtgcc gtcgctggga cgagctgggg aagcaggtca tcgtcctgct
      121 ccaatccagc ggcgagctgc gcgccgacgt cgacccggcg ctgatccatc gcatcctagg
      181 cgacgtctac cacgacacca tcgtcatgtg gctcgagtcg ccggagccgc cattctcact
      241 ccaggatgag cttcgcaggc gcctcaccct cgtggtcgaa ggggttggcc ggaagccgaa
      301 ggagggaaga tgagacgcct gctgttcctg ctcctggcgg cgggccccgc ggccgcccag
      361 gcccccaccg acacgctgca cctgacgctc gatcaggcgg tgcagcgcgc gctggaccgg
      421 agcttcgcca tgcggctcgc ccgcgccgat gtgctcgatg cggacggccg ggtgcgggaa
      481 gcgttctcgg cggcgctgcc gcaggtgacg gggacgctga cctacacgcg acagtttgcc
      541 tcgatcttcc agggcctggg cgggaccgac accagctcgt tcggggacct gttcaagaat
      601 acgccgttcg gcgctgccaa cgcctggaac ttccaggtcc aggcgacgca gctcctgtgg
      661 tccggcggga aggtcgggtc ggggttgtcg gccgcgaagg cggcccggag catcgccacc
      721 ctcgcggcgt cggaaacgac ggccgaggtg agcttccagg tgaagcaggc ctactggaac
      781 gccgcgctgc agagccggct gctcgagatc gctaccggga acctcgatca ggcgcgtgcc
      841 cagctgcgtc aggtgcagtt gtttcgccag gccggcacgc gcgccgagta cgacctgctg
      901 cgggcgcagg tggacgcggc caaccaggag ccggccgtcg tcgcggcgcg caacgacctc
      961 gatctggccg tgctggagct gaaacggctg ctcgatttgc ccgccgacca gccgctcgag
     1021 ctcgcgaccc cgctcgagtc gcccgaggcg accattccgg tcgtcgcgaa cgactcgatc
     1081 ggcgggccgg accggccggc gctggcgcag gcggaggcgg ccgtgctcca gcagaaggag
     1141 gtgctggacg ttgcgcgcgc ggaccgctgg ccgacgctgt cggtcgcgac cacgtacaac
     1201 cagcaggcgt ttccgcaagg cgtgttcccg tccagcgtga atcagttccg gcgcggctgg
     1261 aacggcgaag tgaaactctc gtttccgatc ttcctgggct tccgtaccgg cgcgcgggtc
     1321 gagcaggcgc gcgccgcgct gcagcgggcg gaagcgcagc gggaccaggt gcacaagcag
     1381 gtggagctgg acgtggcgcg ggcgcgcgcc gaggtcgcgc gggcgcaggc gctgctcgtg
     1441 gcgcgccgcg agacggtgcg ccaggcggac cgcgccctgc acatcgcggg cgtgcgctac
     1501 accaacggca tcgccacgca gctcgaggtg tccgacgcgc gggtcgccgc gcagcagtcg
     1561 caggtgaacg aagtacaggc gacgcgggat tacgtagtcg ccctggcccg gttggagcgg
     1621 gccctggggc gggcggttcc cgtggtccag cagccgatca ccccgatcgc gcagtctctc
     1681 aactccgagg aaacacggcc atgaagcgat acacgatgct gggcgcgctc gccgcgctcg
     1741 tgctcaccgg ggccgccgcg gctggatgcg gcaagggtga agcggcgaac aaccccgccg
     1801 actcggtccg caccgcggtg ctcggccccg acgacgtggc gcgcgccgcg cggcgggacc
     1861 tgatcgcggg cgtcccggtc tccgggacgc tccaaccgtc ggtcgaggtg cggatcgcct
     1921 ctccgatccc cgaggtcgtg gagcaggtct tcgtgaagga aggccaggcg gtgcaacggg
     1981 gacaggtgct ggcccggttc cgtaccagcg cggtcgagcc tgccgctctg tccgccgagg
     2041 cgcagcggcg caaggccgag gcggactacg cgcggatgca gaacctctat aaggaaggcg
     2101 ccgtctccga gcaagacgtg gagagcgccg aggtggccct gcgggccgcg caggccaacg
     2161 aggcgggcgc caggaagcgc ctcgatgaag ccacggtgcg cgcgccggcg agcggcgtga
     2221 tcagcaagcg cgccgtcgat tcgggcgacc gggtgaaaga cggcgacctg ctgttccagc
     2281 tcgtggacac gcgcgagctc gagttcgagg ccaccgtgcc gagcgagtac gtgagcgcgg
     2341 tgcgcatcgg cgcccccgtg atgctgaccg tgaccggcgc ggactccggg atcacgggac
     2401 gcgtggcgcg cgtcaacgcg acggtggacg aagcgacgcg gcaggtgaag gtctacctca
     2461 cggtggcgaa ccgcggcggc cggctggtgg gcggcctgtt cgcgaccggc cgtatcgtgc
     2521 tgcaggacgt caagaacgcc gtgaccgtgc cgcgcacggc ggtgcattcc ggcccggacg
     2581 gcaaggccta cgtgctcatc gtcgaagggg gcaagatcgt ccgccgcgac gtcaccatgg
     2641 gcgccgccga cgagcaggcc tctctggtgg agatcaagac cggtcttcag gggggggaaa
     2701 cggtgatcgt aggcccggcg aacgggctcg aggctggtga ccccgcgacg atcgcgggag
     2761 gagagggctg agccatgttt ctgagcgacc tgtcgatcaa gcgcccagtc ttcgcgtcga
     2821 tgatgatgct ggcgctggtg gtgctgggac tgttctccta ccggcggctg aacatcgatc
     2881 agttcccgga cgtggagttt ccgttcctgg tgatccagac ccgctacgcg ggcgcgagcc
     2941 ccgagtcggt ggaacgggaa gtcacgaaga agatcgagga gtcggtgaac accgtggagg
     3001 gcgtgaagcg gatccagtcc acctccacgg aagggttctc gaccatcttc gtgctgttca
     3061 acctcggcac caagctcatg gacgcgcagg ccgacgtgcg cgccaagatc gacgccatcc
     3121 gccaggatct gccgacggat atcgatctcc ccgtgatctc ccgggccaac ccatccgacc
     3181 agccgatctt cgtgctggcg gtgcagggtg agagctgggc gctgcgcgac ctgacccggc
     3241 tcgccgacga ggtggtgagc cgccgcatcg agaacgtctc cggggtcggc agcgtgtccc
     3301 tggtgggcgg cctcaagcgg gagatccacg tgctgctgca gcccgaccgg ctgcaggcgc
     3361 tcggcatctc gcccgacctg gtgacggcgg cactgcgccg cgagaacggc gacgtgccgg
     3421 cggg
//
LOCUS       DBNGZW010000607         8909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1149316, whole genome shotgun sequence.
ACCESSION   DBNGZW010000607 DBNGZW010000000
VERSION     DBNGZW010000607.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8909
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1149316"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3543)
                     /gene="nifJ"
                     /locus_tag="AC16JC_14325"
     CDS             complement(<1..3543)
                     /gene="nifJ"
                     /locus_tag="AC16JC_14325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR5165923.1"
                     /translation="MNPRVATLDGNEAAALVAHRTNEVIAVYPITPSSTMGELADQWS
                     AEGKTNLWGSVPAVIEMQSEGGAAGAVHGALQGGALATTFTASQGLLLMIPNLYKIAG
                     ELTPLALHVAARTIATHALSIFGDHSDVMAVRQTGVALLASASVQEAHDLALVAQAAT
                     LRSRIPMLHFFDGFRTSHEMQKVALLDDDDLRAVLDDGLITEHRRRALDPEHPMLRGT
                     AQNPDAFFQAREACNPFYDACPGIVQQLMDRLGARTGRRYRLFDYTGHPEADRVIVAM
                     GSAVETIEETARRLNLDGDRVGVLKVRLYRPFDAAALLAALPSTVRRIAVLDRTKEPG
                     APGEPLFLDVVTAIADGWSRRSPGTTLPFVVGGRYGLSSKEFTPAMARAVFAELARPE
                     PRRRFTVGIVDDVTGLSLAWDPAFSIEPDDEVRAVFWGLGSDGTVGATKNTIKIIGEE
                     TPLYAQGYFVYDSKKAGAVTVSHLRFGPRPIHAPYLISSATFLACHQFDFLDRYDVLE
                     SAAPGATLLLNAPFAADEVWDRLPREVQETIAAKRIRLFAIDAARVAKEAGLGGRINT
                     VMQTCFFAVSGVLPREQAIGKIKEAIARTYGKRGTAVVERNFAAVDRTLAALGEIAVP
                     GTVTATRGRPPIVAENAPDFVRRVTAVMMAGKGDLLPVSAFPVDGTWPTATSRWEKRN
                     LADEIPTWDAQLCIQCNWCALVCPHAAIRTKVYDPAALAGAPEGFAHVSWRGPDLKGQ
                     AYTVQVAPEDCTGCSLCASVCPATDKTDRTRKAIAMSPQRPIRDRERTAWQFFLGLPE
                     IDRTALTRVNVKTSQLFQPLFEFSGACAGCGETPYLKLLSQLFGDRAVIANATGCSSI
                     FGGNLPTTPWTVDRSGCGPAWANSLFEDNAEFGFGLRLAIDARRDEARRLVTELTPLL
                     GDALAARLANAGDRSEAAIAAGRQALAELRTRLAAANDPRAARLLELSVALLHRSVWL
                     VGGDGWAYDIGYGGLDHVLAQDRDVNVLVLDTEVYSNTGGQQSKATPLGAAAKFATAG
                     KSTGKKDLGLEAMSYGHVYVARVALGADMNQTVRAFAEAESYPGPSLIIAYSPCIAQG
                     YDLSHGLEQQQLAVKSGAWPLYRFDPRRTAEHLAPLQLDSKDPTVSLAAYQRIETRFH
                     AAEQLDPARYERLMAQAEREVADRWAVYRYLAGMA"
     gene            complement(3588..3926)
                     /locus_tag="AC16JC_14330"
     CDS             complement(3588..3926)
                     /locus_tag="AC16JC_14330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165924.1"
                     /translation="MRIVFKSSIVVTTWHPGSDSTRHTHPGVLEASGTIQAAAGDTIA
                     LRLGELRTAAGPVPSISDQVAMLPTAQIARLEERRFQAGTTALAGGAALALAATTFLV
                     VIIVAITHGF"
     gene            4288..5433
                     /locus_tag="AC16JC_14335"
     CDS             4288..5433
                     /locus_tag="AC16JC_14335"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012191670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HIR5165925.1"
                     /translation="MAGTTSAPRGLDGVVAVRTRLSHVDGLAGELIIGGYELKDLAGR
                     VTFEEAAHLLWRGSLPTADQLHGLRREMAALREVPAETMEVVRAASGAPPVDALRMAC
                     ATLSLDLARPDDISPSADLAAATMLTARFPTLVAAHARLNQGLEPVAPRSDLSHAANF
                     LYMAHGTEPDPIAARALDTYWVTVIDHGMNASTFTSRVIASTRSDMVSAVTGAVGALK
                     GPLHGGAPGPVLDMLVDIGSAERAEAWVRKELDAGRRIMGFGHRVYKVRDPRAEVLSR
                     VADEMGRGHLEDRKLFELARSVEQTALKVLEEVKPGRNLRTNVEFYTALVLQALGLAP
                     RSFVALFACGRVAGWCAHVIEQHAEDHLIRPQSEYVGPRGLTTVSSS"
     gene            complement(5430..6158)
                     /locus_tag="AC16JC_14340"
     CDS             complement(5430..6158)
                     /locus_tag="AC16JC_14340"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding periplasmic protein"
                     /protein_id="HIR5165926.1"
                     /translation="MIDSERRSETLPTSPRGVLRVGVDESPVPPLCFGPPGTAEFRGF
                     EPDLLDAIAGRIGLALRCEVVGWSDALVQLQHGRLDMLCRAVTITPERRHLADFSDPY
                     LETELTLVVRRDSPIRDPGDLIGLSVGLRRATVAEEFVRETCPAATVRTYEMHAEPYR
                     ALADRAVDAVVDHTQIATYFARAEVGLRVSGALEGTELECGMVFAPGNDPLRRSVNRA
                     LAQLRADGTWEGLRRRWLSEAETH"
     gene            complement(6253..6325)
                     /locus_tag="AC16JC_14345"
     tRNA            complement(6253..6325)
                     /locus_tag="AC16JC_14345"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6290..6292),aa:Lys,seq:ttt)
     gene            6535..7008
                     /locus_tag="AC16JC_14350"
     CDS             6535..7008
                     /locus_tag="AC16JC_14350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165927.1"
                     /translation="MLHLLALWAHFSRHTVWLSVTLTFVHLVGILVGGGLAIATDRAS
                     LRLSPAAAPDWPQELARLAGVHRWVVAGLALIFASGFLMMLTSFEVFATSAVFWTKMG
                     LVALLLGNGFVRMRAETALHTGRATAWRRFRRTSVASLVLWFVILLAGTLLHTTT"
     gene            complement(7046..7831)
                     /locus_tag="AC16JC_14355"
     CDS             complement(7046..7831)
                     /locus_tag="AC16JC_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR5165928.1"
                     /translation="MTHPGFEAQVDAYLDGELATADARELEAHLAQCVECARFRDGRL
                     DLRAAIAAGIPAFQAPDVLRQRVRAALKTAALARAPYRLGLGGVWRPLAVAASLAVVA
                     FGSWNLSLRHAAGEALADDILASHVRSLMPGHLTDVLSSDQHTVKPWFNGRLDFSPPV
                     YDFAGRGYPLLGGRLDYVDGRTVAALAYGRRQHLINVFLWPATRGPRNAPRSRDRQGY
                     HLLHWATPDYTYWVVSDLGLAELHDFAHLVQQADSATAVPSPR"
     gene            complement(7833..8360)
                     /locus_tag="AC16JC_14360"
     CDS             complement(7833..8360)
                     /locus_tag="AC16JC_14360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5165929.1"
                     /translation="MDSSALGRFEAAVLPHLDAAYTLACYLVRNEHEAEDVVQDACLR
                     ALKYFDGFRGEGETSARAWLLTIVRNTAYSRKGRRRADALTTEFDEMQHSDAVADEHP
                     EGVLLREDAKETLGRALDRLAPEFREVIVLRELEGLSYKEISEVAGVPVGTVMSRLSR
                     ARTRLQEALRNEEGS"
     gene            8561..>8909
                     /locus_tag="AC16JC_14365"
     CDS             8561..>8909
                     /locus_tag="AC16JC_14365"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5165930.1"
                     /translation="MFAGTPAHRSPGDPAAVSAKLSEWKVELSQGTLTAGTVTFTVTN
                     AGSIPHAFEVEGQGIEQETDVIQPGSSATLKLTLKPGTYDVYCPVGADSHKHLGMETH
                     LRVVSAQGTGTSGY"
BASE COUNT         1433 a         3076 c         3021 g         1379 t
ORIGIN      
        1 cgccattccc gcgagatagc ggtacacggc ccagcggtcc gcgacctcgc gctcggcctg
       61 cgccatcagc cgctcgtagc gcgccgggtc gagctgctcc gctgcgtgga agcgagtctc
      121 gatccgctgg taggccgcca gggagacggt cggatccttc gaatcgagct gcagcggcgc
      181 caggtgctcc gccgtgcggc gcggatcgaa gcgatacagg ggccacgcgc cggacttcac
      241 cgccagctgc tgctgctcca ggccgtggga gaggtcgtag ccctgcgcga tgcagggcga
      301 gtaggcgatg atgagcgacg gcccggggta gctctccgcc tcggcgaagg cgcggaccgt
      361 ctggttcata tccgcgccga gcgccacccg ggcgacgtag acgtgcccgt agctcatcgc
      421 ctcgagcccc aagtccttct tgccggtgct cttccccgcg gtggcgaact tcgcggcggc
      481 gccgagcggg gtggccttgg actgctggcc gccggtgttc gagtagacct cggtatccag
      541 caccaggacg ttgacgtcgc ggtcctgggc caggacgtga tcgagcccgc catacccgat
      601 gtcgtacgcc cagccgtctc cgcccacgag ccacacggac cggtgcagca gcgctaccga
      661 cagttcgagg agccgcgcgg cccgcgggtc attcgcggcc gcgagtcgcg tgcgcagctc
      721 ggcgagcgcc tgacgaccgg cggcgattgc cgcttcggag cggtcgccgg cgttcgccag
      781 acgcgccgcg agcgcatcgc ccagcagcgg cgtcaactcg gtcacgagcc gccgagcttc
      841 gtcccgccgg gcgtcgatcg cgagccgcag cccgaagccg aactccgcgt tgtcctcgaa
      901 caacgagttg gcccacgcgg gtccgcaccc cgagcgatcc accgtccacg gggtggtcgg
      961 gaggttgccg ccgaaaatcg aggagcaccc ggtcgcgttg gcgatcacgg cgcggtcccc
     1021 gaacagctgg ctcagcagct tcaggtacgg cgtctcgccg cacccggcac aggcgcccga
     1081 gaactcgaac aacggctgaa agagctgcga ggtcttgacg ttcacgcgcg tcagcgcggt
     1141 ccggtcaatc tcggggagcc cgaggaagaa ctgccaggcc gtgcgctccc gatcgcgaat
     1201 cggccgttgc ggcgacatcg cgatcgcctt gcgggtccgg tccgtcttgt ccgtcgcggg
     1261 gcacaccgag gcgcagagcg agcagccggt gcagtcctcg ggcgccacct gcacggtgta
     1321 cgcctgccct ttgaggtcgg ggccccgcca ggagacgtgc gcgaagcctt ccggcgcgcc
     1381 ggcgagcgcc gcggggtcgt agaccttggt gcggatcgcg gcgtgggggc agaccagcgc
     1441 gcaccagttg cactggatgc acagctgcgc gtcccacgtc gggatctcgt cggcgaggtt
     1501 gcgtttctcc cagcgtgacg tggcggtggg ccaagtgccg tccaccggga acgcgctgac
     1561 cgggagcagg tcccccttgc cggccatcat caccgccgtc acccgccgca cgaagtcggg
     1621 cgcattctcg gcgacgatcg gcggccgccc gcgcgtcgcc gtcacggtgc ccggaacggc
     1681 aatctcgccg agcgccgcga gggtccggtc taccgccgcg aaattccgct cgaccaccgc
     1741 ggtcccccgc ttgccgtagg tccgggcgat cgcctccttg atcttgccga tcgcctgctc
     1801 gcgcggcagc acgccagaca cggcgaagaa acaggtctgc atcaccgtgt tgatgcgacc
     1861 gcccagtccc gcctccttcg ccacgcgagc cgcatcgatg gcgaagagcc gtatccgttt
     1921 ggcggcgatc gtctcctgca cttcccgcgg cagccggtcc cacacctcgt cggccgcaaa
     1981 gggagcgttc aggagcagcg tcgctcccgg ggcggcggat tcgagcacgt cgtacctgtc
     2041 caggaagtcg aactgatgac aggcgaggaa agtcgcgctg ctgatcaggt acggcgcatg
     2101 aatcgggcgg ggcccaaagc ggaggtgcga caccgtcacg gccccggcct tcttggaatc
     2161 gtagacgaag tagccctggg cgtagagcgg cgtctcctcc ccgatgatct tgatcgtgtt
     2221 cttggtggcg cccaccgttc cgtcgctgcc gagtccccag aacacggccc gcacctcgtc
     2281 gtccggctcg atcgagaagg cggggtccca ggccagcgac agcccggtga cgtcgtccac
     2341 gatgccgacc gtgaaacgcc gccgcggctc gggacgcgcc agctccgcga acaccgcgcg
     2401 cgccatcgcg ggcgtgaact ccttggacga gagaccgtag cgtcccccca cgacgaaggg
     2461 gagcgtcgtc ccgggcgagc ggcggctcca tccatcggcg atggccgtca cgacgtccag
     2521 gaacagcggc tcacccggcg ccccgggttc tttcgtccga tccaacaccg cgatgcggcg
     2581 gaccgtcgac ggcaacgcgg ccagcagggc ggccgcgtcg aacggccggt acagcctgac
     2641 cttcagaacg cccacccggt ccccgtccag attgagccgg cgcgccgtct cctcgatcgt
     2701 ctcgacggcg gagcccatcg cgacgatcac ccggtcggcc tcggggtggc cggtgtagtc
     2761 gaacagccgg tagcggcggc cggtgcgcgc gcccagccgg tccatcagct gctgtacgat
     2821 gcccgggcag gcatcgtaga acggattgca ggcttcgcgc gcctggaaga aggcgtccgg
     2881 gttctgcgcg gtgccgcgca gcatggggtg ctcgggatcg agggcgcgcc gacgatgctc
     2941 ggtgatcaag ccatcgtcga gcacggcgcg aagatcatcg tcgtccagca gggcgacctt
     3001 ctgcatttcg tgcgacgtcc ggaagccgtc gaagaagtgc agcatcggga tgcgggagcg
     3061 gagcgtggcg gcctgcgcca cgagcgccag gtcgtgggct tcctgcaccg acgccgaagc
     3121 gagcagggcg accccggttt gacgcacggc catgacgtcg gagtgatcac cgaagatgga
     3181 aagcgcatgc gtggcgatcg tgcgggccgc gacgtgcagc gcgagcgggg tcagctcgcc
     3241 cgcgatcttg tagagattgg ggatcatgag gagcaggccc tgcgacgccg tgaaggtggt
     3301 cgcgagggcc ccgccctgca gcgccccatg gacggcaccc gcggcgccgc cctcggactg
     3361 catttcgatc acggcgggaa cgctgcccca cagattggtc ttcccctcgg cggaccactg
     3421 gtccgccagc tcacccatgg tcgacgacgg ggtgatcgga tagaccgcga tgacttcatt
     3481 ggtacggtgg gccacgaggg cggcggcctc gttgccatcc agggtggcga cgcgagggtt
     3541 catgaaggcg aacctagaga ctgcagaatg aagaaaacgt gaaaatccta gaacccgtgc
     3601 gtaatggcaa cgatgatgac gaccaggaag gtcgtagccg caagggcgag cgccgctccc
     3661 cctgcgagag ccgtggtccc ggcctggaaa cgtcgctctt cgaggcgtgc gatctgagcc
     3721 gtgggcagca tcgcgacctg gtcagagatg ctggggactg ggccggctgc cgtgcggagc
     3781 tcgccgagac gcagggcaat cgtatcgccg gcggcagcct ggatggtgcc gctggcctcc
     3841 agcaccccgg gatgggtgtg ccgtgtgctg tccgagccgg gatgccaggt ggtgacgacg
     3901 atcgagctct tgaacacgat tcgcacacgc gatccaggcg taggggcgag tgcgactggc
     3961 cggtaggcat agcaggcgct ggtagtgagc cacgctccaa agagcgcagc tcgaacggtt
     4021 gcgcgcatcc tgaatcccct gcctgacgaa gtgtgcgtaa gctgggactt acgagcgttc
     4081 gcagtcaagg cagccccgtt atctgcctcc aatccaatga atccgcatac gccgtcgcgg
     4141 aagccacgca gtccggacag tcgactgacg cctcccggaa tacctttcct gggacttggg
     4201 tcattccagg aggcgtcgct ctccggcccg accgcggcgc cgtctggcag tacgagccca
     4261 gaatccgagc cacgaagggg gacgcgcatg gccgggacta cctcagcccc acgggggctg
     4321 gacggcgtcg tcgccgtccg cactcgcctg agccacgtcg atggcctggc cggcgagctg
     4381 atcatcggcg gctacgaatt gaaggacctg gccgggcggg tgacgttcga ggaggcggcg
     4441 cacctgctgt ggcggggctc gctgccgacc gcggatcaac tccacgggct gcgccgggag
     4501 atggcggcgc tccgcgaggt cccggccgaa accatggagg tcgtacgcgc ggcctccggc
     4561 gcgccccccg tagacgcgct ccgcatggct tgcgcgaccc tgtcgctcga tctcgcccgt
     4621 cccgacgata tctcgccgtc cgccgatctc gcggccgcca ccatgctgac ggcgcgcttc
     4681 cccaccctgg tggcggccca tgctcggctc aaccagggcc tcgagcccgt cgcgccccgc
     4741 agcgatctct cccatgccgc gaacttcctc tatatggcgc acggcacgga gcccgatccg
     4801 atcgcggccc gcgcgctcga cacgtactgg gtgaccgtca tcgaccacgg catgaatgcg
     4861 tccaccttca cgagccgggt gatcgcgagc acccggtcgg acatggtgag tgccgtcacc
     4921 ggcgcggtgg gtgcactcaa gggtccacta cacggcggcg cgcccggccc ggtgctcgac
     4981 atgctggtgg acatcggctc ggcggagcgg gccgaggcct gggtccgcaa ggagctcgac
     5041 gcgggccggc gcatcatggg gttcggtcac cgggtgtaca aggtccgcga tccgcgtgcc
     5101 gaggtgctga gccgcgtggc ggacgagatg ggtcgaggcc acctggagga ccgcaagctt
     5161 ttcgagctgg cgcggtcggt agagcagacc gctctcaagg tcctggagga ggtgaagccg
     5221 ggccgcaacc tgcggaccaa cgtggagttc tacacggcgc tggtgctcca ggcgttgggc
     5281 ctcgcaccgc gctcgttcgt ggcgctgttt gcctgcggac gggtggcggg ctggtgcgcc
     5341 cacgtgatcg agcagcacgc cgaggaccac ctcattcgcc cgcaatcgga gtacgtgggg
     5401 ccgcgagggc tgaccaccgt ctcgtcgagc tagtgggtct cagcctccga gagccaccgc
     5461 cggcggaggc cttcccatgt tccgtccgcc cgcaattgcg cgagtgcccg gttcacggat
     5521 cggcgcagtg ggtcgttgcc gggagcgaag accatgccac actccagctc cgtgccctcc
     5581 agcgcgcctg acacgcgcag ccctacctcg gcgcgcgcga agtacgtcgc aatctgcgtg
     5641 tgatcaacga ccgcatccac agcccggtcg gccaatgccc gatacggctc cgcgtgcatc
     5701 tcgtacgtgc gcacggtggc ggccgggcat gtctccctca cgaactcctc ggctacggtc
     5761 gcccgccgca gccccacgct cagtccgata aggtccccgg gatcccgaat cggactgtcc
     5821 cgacggacga ccagggtcaa ttcggtctcg agatacggat cgctgaagtc ggcgaggtgg
     5881 cgccgctcgg gcgtgatggt caccgctcgg cacaacatgt cgagccgccc atgctgaagc
     5941 tgcaccagcg catcgctcca gccgacgacc tcgcatcgca gtgccagccc gatcctgccg
     6001 gcaatggcgt caagcagatc cggttcgaat ccgcggaact ccgctgttcc cggaggaccg
     6061 aaacacagcg gtgggaccgg cgattcgtcg acaccgacgc gcaggactcc acgcggcgaa
     6121 gtcggcagcg tctcagacct gcgttcagag tcgatcatcg gtgcgtgtat gatgcaggag
     6181 acgtgaatcg cgcaacgcct cggggacaag aaactgagtc ataggttagt catagctgat
     6241 tctatacctc gatgaccggg gcggggatcg aacccgcggc ctgcggatta aaagtccgct
     6301 gctctaccaa ctgagctacc cggtcggggg gcgcttcaac ttacagaatc agtgaagtgc
     6361 tcggaagcgc tgcgggtact cacggcccag cttctctcgc tcatgaatca atcgtcatgg
     6421 caacgaaaga gaccccggtc actagcggag cattcccgtg gaatatcgga gggcgccgga
     6481 cggtctaggg ggtcggaagc cggtaatccc gaaccctctt gaaggacgac cacgatgctt
     6541 catctcctcg cgctctgggc gcacttctcc cgccacaccg tgtggctgag cgtgacgctc
     6601 acctttgtcc atttggtcgg catcctggtg ggaggcggct tggccatcgc caccgatcgc
     6661 gcctccctgc ggctctcgcc agcggctgcg ccagactggc cacaagagct cgcccgcctt
     6721 gccggcgttc accgctgggt ggtggcaggc ctcgccctca tctttgcgag cggcttcctc
     6781 atgatgttga cctcgttcga ggtctttgcc acctcggccg tgttctggac caagatgggc
     6841 ctcgtcgcgc tcctgctcgg caacggcttc gtccggatgc gcgcggagac ggcgctgcat
     6901 acgggacgcg ccaccgcatg gcgtcgcttc cggcgcacat cagtggcgag cctcgtgttg
     6961 tggtttgtga tcctgctggc cggtacgctg ctccacacga caacctgacc gtgcgggcgc
     7021 cgtgcagccc cgcgcatgga gcgagctagc gaggtgaggg gacggccgtc gccgaatcgg
     7081 cctgctgcac caggtgggcg aaatcgtgga gctccgccaa gcccaggtcc gagacaaccc
     7141 agtacgtgta gtccggcgtg gcccagtgca gcaggtgata gccctgacgg tcgcgggacc
     7201 tgggcgcgtt gcgcgggccg cgcgtcgccg gccacaggaa gacgttgatc agatgctggc
     7261 gccgcccgta cgcgagcgcc gcgaccgtcc gcccatccac atagtcgagt cggcctccga
     7321 gcagcgggta tccccgaccg gcgaagtcgt acaccggggg agagaagtcg agcctgccgt
     7381 tgaaccaggg cttcaccgtg tgctgatccg acgagagcac gtccgtgagg tggccgggca
     7441 tgagcgagcg gacgtggctc gccaggatgt cgtcggcaag cgcttcgcca gccgcgtggc
     7501 ggagcgagag gttccagctc ccgaacgcga cgacggcgag tgacgcggcc acggccagcg
     7561 ggcgccacac gcccccgagg ccgaggcggt atggggcgcg cgccagcgcc gccgtcttga
     7621 gggccgcccg cacgcgctgc cgcaggacgt ccggtgcctg aaacgcgggg atgcccgcgg
     7681 cgatcgccgc acgcaggtcg agccggccgt cgcggaaccg cgcgcactcg acgcattggg
     7741 caagatgcgc ctcgagctcg cgtgcgtcgg cggtggccag ctcgccgtcg aggtacgcgt
     7801 ccacctgtgc ttcgaagccc ggatgtgtca tctcaactcc cctcctcgtt acgcagcgcc
     7861 tcctggaggc gcgtgcgggc gcgcgacagg cgtgacatca ccgtgccgac cggcacgccc
     7921 gcgacctcgc tgatctcctt gtacgacagt ccttcgagct cgcgcagcac gatcacctca
     7981 cgaaattccg gcgccaggcg gtcgagcgcg cgaccgaggg tttccttcgc gtcctcccgg
     8041 agcagcacgc cctccgggtg ttcatccgcc acggcgtcac tgtgctgcat ctcgtcgaac
     8101 tccgtggtga gcgcgtcggc gcgacggcgc cccttcctgc tgtaggcggt attgcgcacg
     8161 atcgtgagca gccaggcgcg cgcactcgtc tccccctccc cgcggaagcc gtcgaaatac
     8221 ttgagcgccc gcaggcaagc gtcctgcacg acgtcttcgg cctcgtgctc attcctcacc
     8281 agatagcacg ccagcgtgta cgcggcatcc aggtgcggca agacagcggc ttcgaatcgc
     8341 cccagcgcgc ttgagtccac ccggccctca cgaggcctcg tcggccttca ggagaagact
     8401 tcgcagatag caggtttatt cccgacggaa taaccccgcc aggcctcgag tctccgactt
     8461 gaaccacact gccgtccact ttctcgggag aagatcacgt atgcgctcca tgcacgctat
     8521 gccatcgcgc aatgtcgccg ggctcctgtt cggtgccctg atgttcgccg gtacccccgc
     8581 acaccgctcc ccgggcgatc cggcagcggt gagcgccaag ttatccgagt ggaaggtcga
     8641 gctgtcgcag ggcaccctca ccgcgggcac cgtgactttc accgtcacga acgccggcag
     8701 catcccacac gccttcgagg tcgagggcca gggaatcgag caggagactg acgtcataca
     8761 gccgggatcg agcgccacgc tgaagctcac gctcaagccc gggacctacg acgtgtactg
     8821 cccggtgggg gcagactcgc acaagcacct gggcatggag acccaccttc gggtggtcag
     8881 cgcccagggc acgggcacct cgggctacg
//
LOCUS       DBNGZW010000608         1992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1149319, whole genome shotgun sequence.
ACCESSION   DBNGZW010000608 DBNGZW010000000
VERSION     DBNGZW010000608.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1992
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1149319"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /gene="plsY"
                     /locus_tag="AC16JC_14370"
     CDS             complement(<1..569)
                     /gene="plsY"
                     /locus_tag="AC16JC_14370"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012503223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR5165931.1"
                     /translation="MAALPWLLASYLLGAVPTSYLAGRLFRGIDLREHGSRNLGATNL
                     YRVLGWRYAIPVGLFDAAKGLVPVLVFAPRVSTSELFALICGLVAVVGHVFSVFVGFK
                     GGKGVATAAGVMLGLTPAALGVAVVVWVALVYLTGYVSVGSIVAAAIFPLAVYLIDPP
                     DRPAILWLDIAVAAAIVWFHRANIQRLLKG"
     gene            complement(569..1891)
                     /gene="der"
                     /locus_tag="AC16JC_14375"
     CDS             complement(569..1891)
                     /gene="der"
                     /locus_tag="AC16JC_14375"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012811012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR5165932.1"
                     /translation="MSKPTVAIVGRPNVGKSTLFNRIIGGRPAIVSDRPGTTRDRHFG
                     DAEWQGRQFWVVDTGGLVPESDDSMDRAIRQQVDFALAESDVVLFVVDGRDGLNPVDK
                     AIAERLRKAQRPVVLAVNKLDDLERSTAQFDFYQLGFGDPLGVSAAVGKGSGDLLDAV
                     VERLPPFDPAEMEASINVAVVGRPNAGKSSLVNKLLGEERHVVAPEAGTTRDAIDSLL
                     RYRGKSLKFIDTAGLRRRAKVEDDLEFYSTLRTERAIERAEVCVLVVDATLGMHNQDL
                     RIATEAWDQGCGLIVVVNKWDLVEEKDANTARRGQEELVEKAPFLRYVPFVYVSALTG
                     QRVRRILDLILEVAEGREQRVPTAEVNRVLTSLLERNSPPQKPGEEVKLLYASQIGVA
                     PPTIAIVSNRPDDVPDAYERYLVHGFREAWAFTGSPLRLKFTARGSKR"
BASE COUNT          330 a          719 c          654 g          289 t
ORIGIN      
        1 cccttcagca gccgctggat attggcccgg tgaaaccaca cgatcgccgc cgcgaccgcg
       61 atgtcgagcc acaggatcgc cggccgatcg ggcgggtcaa tcaggtagac ggccagcggg
      121 aagatggcgg cggccacgat gctgccgacc gagacgtacc cggtgaggta caccagcgcc
      181 acccacacca cgaccgcgac gccgagcgcg gccggggtga gccccagcat cactccagcc
      241 gcggtggcga cgcccttccc gcccttgaat cccacgaaca ccgagaacac gtgccccacc
      301 accgccacga gtccgcagat cagcgcaaac agctccgagg tggagacccg cggcgcgaag
      361 acgagcacgg gaacgagtcc cttcgcggcg tcgaacaggc cgacggggat cgcgtagcgc
      421 cagcccagca cgcggtacag gttggtggcg ccgagattcc ggctgccgtg ctcgcgcaga
      481 tcgatgcccc ggaacagccg ccccgccaga tagctcgtcg gcaccgcgcc gagaaggtac
      541 gaggcgagca gccagggcag cgcggccatc agcgcttcga gccccgcgcg gtgaacttga
      601 gccggagcgg cgagccggtg aacgcccacg cctcgcggaa gccgtgcacg aggtagcgct
      661 cgtacgcgtc gggcacgtcg tcgggccggt tgctcacgat ggcgatcgtc ggcggcgcga
      721 cgccgatctg cgacgcgtac agcagcttca cctcttcgcc cggcttctgg ggcggcgaat
      781 tgcgctcgag cagggacgtc agcacccggt tcacctccgc ggtcggcacg cgctgctccc
      841 gcccttccgc gacctcgagg atcaggtcga ggatccgccg cacccgctgc ccggtgaggg
      901 cggagacgta cacgaacggc acgtaccgga ggaacggcgc cttctccacc agctcctcct
      961 gtccccggcg cgccgtgttc gcgtccttct cctcgaccag gtcccacttg ttgaccacga
     1021 cgatcaggcc acagccctgg tcccaggcct ccgtggcgat ccggagatcc tggttgtgca
     1081 tgcccagcgt cgcgtccacc accaggacac acacctccgc ccgctctatg gcgcgctcgg
     1141 tgcggagggt cgagtagaac tccaggtcat cttctacttt agcacgacgg cgtaagcctg
     1201 cagtatctat gaacttgagg cttttacctc gataccgcaa gagcgagtcg atcgcgtccc
     1261 gcgtggtgcc cgcctcaggg gcgacgacat gccgctcctc cccgaggagc ttgttgacca
     1321 gcgaggactt ccccgcgttg ggccggccca ccaccgcgac attgatcgag gcctccattt
     1381 cagccggatc gaacggtggc agccgctcga ccaccgcgtc cagcaggtcg ccgctgccct
     1441 tcccgaccgc ggcgctcacg cccagcgggt cgccgaagcc gagctggtag aagtcgaact
     1501 gcgcggtgct tcgctcgagg tcgtcgagct tgttgaccgc gagcacgacc ggccgctgcg
     1561 ccttcctgag ccgctccgcg atcgccttgt cgaccgggtt gagcccgtcc cgtccgtcca
     1621 cgacgaagag caccacgtcc gattcggcca gcgcgaagtc cacctgctgc cggatggcgc
     1681 ggtccatgga gtcgtcggac tcgggcacca gcccgccggt gtccaccacc cagaactgcc
     1741 ggccctgcca ctcggcgtcg ccgaagtgac ggtcgcgcgt ggtgccgggc cgatcggaca
     1801 cgatcgccgg gcgcccgccg atgatgcggt tgaacagcgt cgacttgccg acgttggggc
     1861 ggcccacgat ggcgacggtg ggcttgctca tgccgcagcc ggggtgtgga gcgcgtcgat
     1921 gaagtccttg gagaggcgca gctccatcgg cacgccggcg gagaggaggt cgaagctcat
     1981 gctgtccatg aa
//
LOCUS       DBNGZW010000609         5388 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_56659, whole genome shotgun sequence.
ACCESSION   DBNGZW010000609 DBNGZW010000000
VERSION     DBNGZW010000609.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5388)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5388)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5388
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_56659"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2231)
                     /locus_tag="AC16JC_14380"
     CDS             complement(<1..2231)
                     /locus_tag="AC16JC_14380"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5165933.1"
                     /translation="MSDAPGPLTAALAERYALEHELGRGGAATVYLAEDRKHRRPVAV
                     KVLRPDLASQLGKERFSREIAIAAQLSHPHILPFIDSGEAAGLLYYITPYITGESLRQ
                     RLDREHRLPVRDAVRIACEVAAALDYAHRQGFIHRDVKPENVLLSDDHAVVADFGIAR
                     AIAEAGGGEPITERGLAIGTPEYMSPEQASAEGQLSARSDLYSLGCMLYEMLAGEPPF
                     TGPNARRILARHVAERARPLRAARPDTPPAVEEAVTRLLAKDPAERFATGAEVVAALQ
                     DGRVAAPRATFGLARFIAVLPFANASPESDAEYLSDGITDELINALTKVEGLRVASRT
                     SVFALKGKPQDVRATGALLGVSAVLEGTVRQAGGRLRVTASLTATDDGRQLWSERYDR
                     PVGDVFAIQDDIAHTIVNTLRATYLADLAEPAPTQHTESLEAYGLYLKGRYSWNKRSL
                     TGAAEAITYFEQAIVADPRFALAYAGLADAYALQVDYRGAPVREGFELAKRYARQALA
                     LDPTLAEVHSSLGWVLFIYDWDWEAATAAFQRALEIDPGYATAHQWYSFVVMTQRSID
                     QALVEGHTALELDPASTSIRRSLGWLYYYARRNDDALRHLRRAIAMDPTSEENYRMLG
                     RVHTQRGEYTEAERALREAVSLSPETAYATGTLGYLYAVRGDRAAAERTVAELEARRK
                     TGYVSPVAICMVYAGLGMADEVFAWLERAYEERRGWLAYLKVEPALDGLRGDPRFTAL
                     LRR"
     gene            2493..3485
                     /locus_tag="AC16JC_14385"
     CDS             2493..3485
                     /locus_tag="AC16JC_14385"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR5165934.1"
                     /translation="MTDTIGTTDHLTADSRRRRYRDVRELIASPENPTPLVRLNRVLP
                     PGMDAYLKLEWFNPFGSIKDRTARALLEGMRRRNELRDKELVEATSGNTGIALAALAA
                     LDGLRLTVTIPSGVPEEKLVLLRMLGATVLPTPDELCPVDNPRDGAIALARAIAESPA
                     HAGRYAMPDQYANPDNVRAHYENTGPEIWEQTEGQVRTFVAGYGTCGTITGVARFLKE
                     RDASIRVVAVEPQPGHRLPGLKNLEEARAPAILDRSVIDEVIRVDDGPAYRMAQRLFR
                     EEALIVGPSTGAIVQAIATLADATGPIVGISPDGGQKYASFYADLVGDSDAVVI"
     gene            3487..3732
                     /locus_tag="AC16JC_14390"
     CDS             3487..3732
                     /locus_tag="AC16JC_14390"
                     /inference="COORDINATES: protein motif:HMM:NF013379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR5165935.1"
                     /translation="MAALPADAELDCKGLLCPLPAYKTRLALARLHPGQVLRVECTDP
                     GSLADIPALARQSRWNLLSVAERDGVQVFVIQRPSLT"
     gene            3947..>5388
                     /locus_tag="AC16JC_14395"
     CDS             3947..>5388
                     /locus_tag="AC16JC_14395"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase domain-containing
                     protein"
                     /protein_id="HIR5165936.1"
                     /translation="MRRLAELDVLRHVSVLSTVSGGSILGGCYILNLKHRYESNWGAL
                     TRRDYEEVVAATEAALRRGVRKNLRTRLLMTPWWNLRMLCSDFSWGRRMARLYQRYLF
                     RDAARRINPAWKDSVPLQELKFRPPGRGMGFDVELHNREAARQAEPTLVPKWVINATS
                     LNTGRDFRFTMVEIGDPELGAIRFDETATVLAYKRLLATAQRRASDGPKWFPDMVTEA
                     REAFLTRDDPWLRHHDAAGAGAGAGRPAAGALEHHPFREFTAAHLYWWVAARDGAAWK
                     DVSGRAAIPDTVFALAGDGREVLRALAKVEFGQLRKAKLAAWYLRDGLHRDPPVTGGY
                     TREQHIDHLWDALRAALPGRDDLRQRLHGNIFDQFLELVIDVYYFRSAERLSWAMARE
                     LERLTLADAVAASANFPPVFNPYMVSGLYDPATTHRLALTDGGVYDNQGLSALVEEEC
                     THIVASDAGGSLPLERRPSAGRIPMLARIGA"
BASE COUNT          783 a         1902 c         1896 g          807 t
ORIGIN      
        1 atccggcgca gcagcgcggt gaagcgcggg tctccccgca ggccgtcgag ggcgggctcg
       61 actttgagat aggcgagcca cccgcggcgt tcctcgtagg cgcgttccag ccaggcgaac
      121 acctcgtcgg ccatgccgag gccggcgtag accatgcaga tcgcgaccgg ggacacgtac
      181 ccggtcttgc ggcgcgcttc cagctccgcc acggtgcgct ccgccgcggc gcgatcgccg
      241 cgcacggcgt acagatagcc gagggtcccg gtggcgtacg ccgtctcggg cgagagcgac
      301 actgcttcgc gcagcgcgcg ctcggcctcg gtgtactcgc cgcgctgcgt gtgcacgcgg
      361 cccagcatgc ggtagttctc ctccgacgtc ggatccatgg cgatggcgcg gcgcagatgc
      421 cgcagcgcgt cgtcgttgcg gcgcgcgtag tagtacagcc agccgagcga gcggcggatc
      481 gacgtcgagg cggggtccag ctcgagggcg gtatggcctt ccaccagcgc ctggtcgatg
      541 ctgcgctgcg tcatgacgac gaagctgtac cactgatgcg ccgtcgcgta gcccggatcg
      601 atctccagcg cgcgctggaa cgccgcggtc gcggcctccc agtcccagtc gtagatgaac
      661 agtacccagc ccagggagct gtgcacttcg gcgagcgtgg ggtcgagtgc cagcgcctgg
      721 cgggcatacc gcttcgccag ctcgaaaccc tcccgcaccg gcgcgccgcg gtagtcgacc
      781 tggagggcat aggcgtccgc gagacccgcg tacgccaggg cgaatcgggg atccgccacg
      841 atcgcctgct cgaagtaggt gatcgcttcc gccgcccccg taagagagcg cttgttccag
      901 ctgtatcgcc ccttcaggta gagcccgtac gcctcgagac tctccgtgtg ctgggtcggc
      961 gccggctcgg ccagatcggc gagataggtg gcgcggagcg tgttgacgat cgtgtgggcg
     1021 atatcgtcct ggatcgcgaa tacgtcgccc accgggcggt cgtaccgttc cgaccagagc
     1081 tgccggccgt cgtcggtggc ggtgagactc gccgtgacgc gcagccgccc gcccgcctgc
     1141 cgcaccgtcc cctcgagcac cgcggatacg ccgagcaaag ccccggtggc gcgcacgtcc
     1201 tgaggcttcc ccttgagggc gaacaccgac gtgcgggagg cgacgcggag cccctcgacc
     1261 ttggtgaggg cattgatcag ctcgtcggtg atgccgtcgc tcaggtactc ggcgtcggac
     1321 tcggggctcg cgttggcgaa cgggagcacc gcgatgaagc gcgccaggcc gaacgtggcc
     1381 cgcggggccg cgacccggcc gtcctgcagc gccgccacca cctcggcacc cgtcgcgaag
     1441 cgctccgccg ggtccttggc cagcagacgc gtcacggcct cttcgacggc cggcggtgtg
     1501 tcgggacgcg cggcccgcaa cggccgtgcg cgctcggcca cgtgccgggc gagaatgcgg
     1561 cgcgcattgg gaccggtgaa gggcggctcg ccggccagca tctcgtatag catgcaaccc
     1621 aggctgtaga ggtcgctccg cgcgctcaac tgcccctcgg cgctcgcctg ctccggactc
     1681 atgtactcgg gcgtcccgat ggcgagtccg cgctccgtga tcggctcacc cccgccggcc
     1741 tccgcgatcg cccgcgcaat gccgaaatcg gccaccaccg cgtgatcgtc ggagagcagg
     1801 acgttctcgg gcttcacgtc gcggtgaatg aacccctgcc ggtgcgcgta gtcgagcgcc
     1861 gccgccacct cgcacgcgat gcgcacggcg tcccgcaccg ggagccgatg ctcgcggtcc
     1921 aggcgctggc gcagcgactc gccggtgatg tacggcgtga tgtagtacag cagtccggcc
     1981 gcttctcccg agtcgatgaa cggcaggatg tgcgggtggg acagctgggc cgcgatcgcg
     2041 atttcccggg aaaaccgctc cttgcccagc tgggacgcga gatccgggcg cagcaccttc
     2101 accgccacgg gtcgccggtg tttgcggtct tccgccagat agacggtcgc ggcgccgccg
     2161 cgccccaact cgtgctccag tgcatagcgc tcggcgaggg cggcggtcag gggtcccggg
     2221 gcgtcagaca tatgtaagga tgatacagcc tcaccgggcc cggggaactc gtcgacgggt
     2281 catcgggttc aatcaggtga tgatcgccag ccgctaccac cccggggcct gcgcctgtag
     2341 ccgctgccaa ccagacggca gggcgggccg cgctggccgg aggccgctgg tcccgggtta
     2401 gcccgagcga gcttcgcgat cgatcacttc cgcccgcacg gttcaccgtc gcgggcttgt
     2461 tgtttcgatg gagcatgagg agctgcctga cgatgacgga tacgatcgga accaccgacc
     2521 acctgaccgc tgactcccgc cgccgccgct accgcgatgt gcgcgagctg atcgcctccc
     2581 ccgaaaatcc gacgccgctg gtgcggctga accgggtgct gccgccgggg atggacgcct
     2641 acctcaagct ggagtggttc aatcccttcg gctccatcaa ggaccgcacc gcccgggcgc
     2701 tgctcgaagg aatgcggcga cggaacgagc tgcgcgacaa ggagctggtc gaggcgacct
     2761 cgggcaacac ggggatcgcc ctggcggccc tcgccgcgct cgacggcctg cggctcacgg
     2821 tgaccatccc ctcgggcgtg ccggaagaga agctcgtgct gctgcgcatg ctcggggcga
     2881 ccgtcctgcc cacgccggac gagctgtgcc cggtggacaa cccgcgcgac ggggccatcg
     2941 cgctggcccg cgccattgcc gagagtccgg cgcacgccgg ccgctacgcg atgcccgatc
     3001 agtacgcgaa ccccgacaac gtgcgggcgc actacgagaa cacgggcccc gagatctggg
     3061 agcagaccga ggggcaggtg cgcaccttcg tcgccggcta cggcacctgc ggcaccatca
     3121 ccggagtggc gcgcttcctg aaggagcgcg acgcgagcat tcgcgtcgtg gcggtggagc
     3181 cgcaaccggg acaccgcctc ccggggctca agaacctcga ggaggcgcgg gcgcccgcca
     3241 tcctcgaccg ctcggtgatc gatgaggtga tccgtgtgga cgacggcccc gcctatcgga
     3301 tggcccagcg gctatttcga gaggaggcgc tgatcgtggg gccttcgacc ggcgccatcg
     3361 ttcaggcgat cgccacgctc gcggacgcca ccggcccgat cgtggggatc tcccccgacg
     3421 gcggtcaaaa gtacgcgtcg ttctatgccg acctcgtagg cgactccgac gcggtggtga
     3481 tctgacatgg ccgcgctccc cgccgacgcc gagctcgact gcaagggcct gctctgtccg
     3541 ttgcccgcct acaagacgcg cctggcgctc gcccggctgc atcccgggca ggtgctgcgc
     3601 gtcgaatgca ccgatccggg atccctcgcc gacatcccgg cgctggcgcg ccagagccgg
     3661 tggaatcttc tttctgtcgc cgagcgggac ggcgtccagg tgttcgtcat ccagcgtccg
     3721 tccttgacgt agcagtttcc gtcttccaca ttgcggcgcg ccgtcctcca aggaagctct
     3781 gcgtaagtgg ggcgacgccg cgggtggggg gcgaggacgc gaggaatcga atgcgatcct
     3841 ggcacgggcg tttcgcgaac agggggctcg tctgacgccg ggagccaagc tgggcctggc
     3901 cctctcggga ggcgggtttc gggcctcctt cttccatctc ggcgtcctgc gacgcctcgc
     3961 cgagctcgac gtcctccgtc acgtcagcgt gctgtcgacc gtgtcggggg gctcgatcct
     4021 gggcggctgc tacatcctga atctgaagca ccgctacgaa tcgaactggg gcgccctcac
     4081 ccgccgcgac tacgaagagg tcgtcgccgc cacggaagcg gccctccgcc gcggcgttcg
     4141 caagaacctg cgcacccgcc tgctcatgac gccgtggtgg aacctgcgga tgctgtgcag
     4201 cgacttctcc tggggccggc gcatggcgcg cctgtaccag cgctacctgt tccgcgatgc
     4261 cgcccggcgc atcaatcctg cctggaagga cagcgtgccg ctgcaggagc tgaagttccg
     4321 cccgccgggc agaggcatgg ggttcgacgt ggagctgcat aaccgcgagg ccgcgcgcca
     4381 ggccgagccg acgctcgtcc ccaaatgggt gatcaacgcc acctcactca acaccgggcg
     4441 cgacttccgg ttcaccatgg tcgagatcgg ggatcccgag ctcggggcga tccgcttcga
     4501 tgagacggcg accgtgctgg cctacaagcg gctgctcgcc accgcccagc gccgcgcctc
     4561 cgacggcccc aagtggttcc ccgacatggt caccgaggcg cgcgaggcgt ttctgaccag
     4621 ggacgatccc tggctgcggc atcacgatgc ggcgggagcg ggggcggggg ccggccggcc
     4681 ggcggcgggt gcgctggagc accacccgtt tcgtgagttc acggcggcgc acctctactg
     4741 gtgggtcgcg gcgcgcgacg gcgccgcctg gaaggacgtg tccggacgcg cggccatccc
     4801 ggacaccgtg ttcgcgctgg ccggggatgg acgggaggtg ctgcgcgcgc tggcgaaggt
     4861 ggagttcggc cagctgcgga aggccaagct cgcggcctgg tacctgcgcg acggcctgca
     4921 ccgcgatccg ccggtgacgg gcggctacac gcgggagcag cacatcgacc acctgtggga
     4981 cgcgctgcgc gccgcgctgc cggggcgcga cgatctgcgg cagcgcctcc acggaaacat
     5041 cttcgaccag ttcctcgagc tggtgatcga cgtgtattac ttccgcagcg ccgagcgact
     5101 gagctgggcc atggcgcgcg agctcgagcg cctcacgctg gcggacgccg tggcggcgtc
     5161 ggccaacttc ccgcccgtct tcaatcccta catggtgtcc ggcctgtacg acccggccac
     5221 gacgcaccgc ctggcgctga ccgacggcgg cgtgtacgac aaccaggggc tgtcggcgct
     5281 ggtcgaggag gagtgcactc acatcgtcgc gagcgacgcg ggtggctcgc tgcccctgga
     5341 gcgccggccg agcgccggcc ggattccgat gctggcgcgc atcggcgc
//
LOCUS       DBNGZW010000610         1441 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_328825, whole genome shotgun sequence.
ACCESSION   DBNGZW010000610 DBNGZW010000000
VERSION     DBNGZW010000610.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1441)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1441)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1441
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_328825"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="AC16JC_14400"
     CDS             complement(<1..734)
                     /locus_tag="AC16JC_14400"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HIR5165937.1"
                     /translation="MSSTKYVFVTGGVVSSLGKGIAAASLGRLLAERGLKVTIQKFDP
                     YINVDPGTMSPFQHGEVYVTDDGAETDLDLGHYERFIDRSLSQVNNVTTGRIYLSVIT
                     KERRGEYLGSTVQVIPHITDEIKSAIRRLAPDHDVVITEIGGTVGDIESLPFLEAIRQ
                     FRQDVGRENTLFIHLTLVPYIAATAELKTKPTQHSVRELMQIGIQPDFLICRTERPLS
                     EELKRKIAQFCNVDFGCVVESPDVQT"
     gene            complement(731..>1441)
                     /gene="kdsB"
                     /locus_tag="AC16JC_14405"
     CDS             complement(731..>1441)
                     /gene="kdsB"
                     /locus_tag="AC16JC_14405"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011136891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HIR5165938.1"
                     /translation="SRLPRKPLRLLAGEPLVLRVARRLIALEVCDRVVVATEADEIAG
                     VVRQAGLAATLTAASHESGTARVAEVIDKQEYRAFDLILNVQGDEPLVAAAAVQGTLA
                     RLRAGDPIGTAAGSLDRRLAADPNRVKVVVGPSGRAIYFSRAPIPFDRDGAGEVVYHQ
                     HVGVYGYTREALQHWVRLPPVPEERWERLEQLRPLLHGIAIGVALFDEAPAPGVDTVE
                     DLEWVEQVLTRHPREVNS"
BASE COUNT          225 a          480 c          492 g          244 t
ORIGIN      
        1 atcgtctgca catcggggct ctccaccacg cagccgaaat ccacgttgca gaactgcgcg
       61 atcttgcgct tcagctcttc ggacagcggc cgctcggtgc ggcagatcag gaaatcgggc
      121 tggattccga tctgcatgag ctcccgcacg gagtgctggg tgggcttggt cttgagctcg
      181 gcggtggcgg cgatgtaggg gacgagggtg aggtggatga acagcgtgtt ctcgcgtccc
      241 acatcctggc ggaattgccg gatcgcctcg aggaacggca gcgattcgat gtcgcccacg
      301 gtgccgccga tctccgtgat gaccacgtcg tgatcggggg cgaggcggcg gatcgccgac
      361 ttgatctcgt cggtgatgtg cgggatcacc tgcaccgtcg agccgaggta ttcgccgcgg
      421 cgctccttgg tgatgacgga gaggtaaatc cggcccgtgg tgacgttgtt cacctgggac
      481 agcgagcggt cgatgaagcg ctcatagtgc ccgaggtcga ggtcggtctc cgccccgtcg
      541 tcggtcacgt acacctcgcc gtgctggaac ggcgacatgg tgccgggatc gacgttgatg
      601 taggggtcga acttctggat cgtgaccttg aggccacgct cggcgagcag gcggcccagg
      661 gaggccgctg cgatgccctt gcccagggag ctcaccactc cgccggtcac gaacacgtac
      721 ttcgtcgagc tcacgaattc acctccctcg gatgacgggt caggacctgc tcgacccatt
      781 ccagatcctc caccgtatcg acgcccggcg ccggtgcttc gtcgaacagc gccacgccga
      841 tcgctatgcc gtgcagcaac ggccgcagct gctccagccg ctcccaccgc tcttccggca
      901 cgggcggcag gcgcacccag tgctgcagcg cctcccgcgt gtagccgtag acgcccacgt
      961 gttgatggta gacgacctcg cctgcgccat cccgatcgaa cgggatcggc gcgcgcgaga
     1021 agtagatcgc ccgcccgctc gggccgacca cgaccttgac ccggttgggg tccgcggcga
     1081 gacgccgatc caggctgccc gccgccgtcc cgatggggtc gccggcgcgc aggcgcgcga
     1141 gcgtgccctg caccgccgcg gcggcgacga ggggctcatc gccctggaca ttcaggatga
     1201 ggtcgaatgc cctatactct tgcttgtcaa tgacttcagc cacgcgggcc gtgcccgatt
     1261 cgtggctcgc agcggtgagc gtcgctgcca gtccagcctg tcgcaccacc cccgcgatct
     1321 cgtcggcttc ggtggcgacc acgacgcggt cacacacctc gagcgcgatc aggcgacggg
     1381 cgactcggag gacgaggggt tcaccggcga ggaggcgcag gggcttgcga ggcaggcggc
     1441 t
//
LOCUS       DBNGZW010000611         2456 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1557544, whole genome shotgun sequence.
ACCESSION   DBNGZW010000611 DBNGZW010000000
VERSION     DBNGZW010000611.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1557544"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..536)
                     /locus_tag="AC16JC_14410"
     CDS             complement(<1..536)
                     /locus_tag="AC16JC_14410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165939.1"
                     /translation="MQRRAHSLIAGVLAALAAAEWLRGLAPVWAWVAGVAGLVAAVLA
                     LRGGLPVFRAMLLRVRAVVQGRPLPPGPVLPSGWLASALAGIVSLVLGLGLLLGLRSI
                     RRIECCWPVLREARVTKASNALEATLSDAITECRRLAERAAVAASLPRQEAFDRVAAG
                     LAAAEWLRGLAPVWAWVAG"
     gene            519..1238
                     /locus_tag="AC16JC_14415"
     CDS             519..1238
                     /locus_tag="AC16JC_14415"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF014670.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR5165940.1"
                     /translation="MSAPLHLKHLVPEDIRVALARDPRLLIPVGTCEQHGPHLPLGCD
                     TIIVERLADDLSAEFAVLRAPTVEYGVNTATRRPFPGNAMVRRKTLHRWMNDLLGSWE
                     EAGVEQFIILTAHGHDPHQEALSTLRTNRARVFTVDVFALDVGDLLLDPESPMHGGEL
                     DTSLLLYLAPHLVRMDQARDFLPTGRQERRHVRGLTAPLPALSPGSLGRPTLATAEKG
                     ERLYKMIRERVATRVLAAAEA"
     gene            1235..1927
                     /locus_tag="AC16JC_14420"
     CDS             1235..1927
                     /locus_tag="AC16JC_14420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165941.1"
                     /translation="MILLPLAAALALTQVPSGVIDEGVLVVRRDSQEVARESFHLRER
                     RPGDPSGGWLLDASARWPGPGNRTTVLTPVLELGADTAPAALAYEVVADGKSEHITGQ
                     PGPGRFTLRYLLPGVERARELPGGPHIVIADDSVFSLFLLVAWLAPRGPDSGPTHVTA
                     ILPRMSSRATLTLTDLGIAATTVNRDPALLRHVLIAGGPMGPVHVWLDRDGRLMKIEM
                     PERSLRAERLPG"
     gene            2084..>2456
                     /locus_tag="AC16JC_14425"
     CDS             2084..>2456
                     /locus_tag="AC16JC_14425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165942.1"
                     /translation="MKSTVLWLGLVCAAGTAAAQQRVPATLSLTDALTIARDHNPLYR
                     QTLNNRGPAAWATRNAFANLLIPQVTAQGSLGYSGTGSQRFLTSNFSQSYATVSSSYS
                     LGLDWTVSGSTLSQPGLQKAQL"
BASE COUNT          372 a          935 c          813 g          336 t
ORIGIN      
        1 ccggccaccc acgcccagac cggcgcgagc ccccgcagcc actcggcggc ggcgagcccg
       61 gccgcgacac gatcgaaggc ctcctgccgc ggcagcgagg cggcgaccgc ggcgcgctcg
      121 gccagccgcc ggcattcggt gatcgcgtcg gacaaggtcg cttcgagggc gttcgaggct
      181 ttggtcacgc gggcttcgcg cagcaccggc cagcagcatt cgatgcgccg gatggaccgc
      241 aatccgagca acaggccgag cccgagcacg agggagacga tcccggccag cgcggaagcc
      301 agccagcccg aagggagaac gggacccggc ggcagcggcc gcccctgcac gacggcgcgc
      361 acgcgcagca gcatcgcgcg gaagaccggg agcccgccgc gaagcgccag gaccgccgcc
      421 acgaggcccg cgacaccggc cacccacgcc cagaccggcg cgagcccccg cagccattcg
      481 gcggcggcga gcgcggccag gacgccggca atcagcgaat gagcgcgccg ctgcacctca
      541 agcacctcgt tcccgaagac atccgtgtcg ccctcgcgcg ggatccgcga ctcctcatcc
      601 cggtgggaac gtgcgagcag cacggtcccc acctgccgct gggctgcgat accatcatcg
      661 tcgaacggct cgccgacgac ctctcggcgg agttcgccgt gctgcgcgcc cccaccgtcg
      721 agtacggcgt caacaccgcc acgcgacggc cgttccccgg caatgccatg gtacggcgca
      781 agacgctgca ccgctggatg aacgacctgc tcggctcctg ggaggaggcc ggcgtggagc
      841 agttcatcat cctgacggcc cacgggcacg acccccacca ggaggccctc agcaccctgc
      901 ggaccaatcg cgcgcgtgtc ttcacggtgg acgtgttcgc cctcgacgtc ggcgatctgc
      961 tcctggaccc cgagtccccg atgcacggcg gggagctcga tacgtcgctg ctgctctacc
     1021 tggcaccgca cctcgttcgc atggaccagg cgcgcgattt cctgccgacc ggccgccagg
     1081 agcgccgcca tgtccgcggg ctgaccgcgc cgctccccgc cctgtcgccg ggctcgctgg
     1141 gccgccccac gctggccacg gctgaaaagg gcgaacgcct ctataagatg atccgggaac
     1201 gtgtcgccac gcgagttctc gcggccgccg aggcatgatc ctcctcccgc tggccgcagc
     1261 cctcgcgctc acccaggtgc cgagcggggt gatcgacgaa ggcgtgctgg tggtgcggcg
     1321 cgattcgcag gaggtcgcgc gtgagagctt ccacctgcgc gagcggcgtc ccggagaccc
     1381 ttccggcgga tggctgctgg acgcctccgc gcggtggccg gggccgggca accgcaccac
     1441 ggtcctcaca ccggtgctgg agctgggcgc cgataccgcc ccggcggcgc tcgcctatga
     1501 agtcgtcgcc gatggcaagt ccgagcacat cacggggcaa ccggggccgg gacgcttcac
     1561 cctgcgctac ctgctccccg gggtggagcg tgcccgcgag ctgcccggcg gtccgcacat
     1621 cgtcatcgcc gacgactcgg tgttctcgct gttcctgctg gtggcgtggc ttgcgccccg
     1681 tggtcccgac tccggcccca cccacgtcac cgcgattctg ccccggatgt cgagtcgcgc
     1741 gacgctcacg ctgaccgatc tcgggatcgc cgccacgacg gtgaaccggg atccggcgct
     1801 gctgcgacac gtcctgatcg ccggcggccc catgggcccg gtgcatgtct ggctcgatcg
     1861 cgacggccgt ctgatgaaaa tcgagatgcc cgagcggagc ctgcgcgccg agcggctccc
     1921 cggctgatcc ccctgcaggg aagtcgttga gcgagttagc tttccgcctc aggcctgccg
     1981 ctatttcagc ccgaaacctg actgaggcct aacgcgtaca ggccggggct ttcggtacgt
     2041 tcagaaggag tcccatcacc ttcgcccgtc agcaaggact cgcatgaaat cgaccgtgct
     2101 ttggctgggt ctcgtctgcg ccgccggcac cgccgcggca cagcagcgcg tccccgccac
     2161 cctgtcgctc accgacgcgc tcaccatcgc gcgagaccac aatccgctct accgacagac
     2221 gctgaacaac cgcgggccgg ctgcctgggc gacgcggaac gcgttcgcca acctgctcat
     2281 tccgcaggtg acggcgcagg ggagcctggg gtactcgggc accggatcgc agcgcttcct
     2341 gacctcgaac ttctcgcagt cgtacgcgac ggtcagctcg agctactcgc tcgggctcga
     2401 ctggaccgtc tcgggctcca ccctgtcgca gcccggcttg cagaaggcgc agctga
//
LOCUS       DBNGZW010000612         2118 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_398945, whole genome shotgun sequence.
ACCESSION   DBNGZW010000612 DBNGZW010000000
VERSION     DBNGZW010000612.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2118)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2118)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2118
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_398945"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..787)
                     /locus_tag="AC16JC_14430"
     CDS             complement(<1..787)
                     /locus_tag="AC16JC_14430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HIR5165943.1"
                     /translation="MTAPPPVPALVIQTAFLGDVVLTTPLLVVLAERHGPVDVVTTPA
                     AAPLLETHPAVRQVLVYDKRKADRGLAGLRRLARRLRAERYAVAYLPHRSLRSAALAR
                     LARIPRRVGYSDGWRSLYTEARPRPRDGHEVDRLLALVDATPHHGARPTLQLAANDVA
                     RAEAAIRSAAVAPPFAALAPGSIWGSKRWPHFAALARRLARQLDVVLVGGPDDAALAE
                     AIVAAAPEGDAAHRVVSLCGRLSLRESAAVIREAAILVTNDSAP"
     gene            complement(809..1351)
                     /locus_tag="AC16JC_14435"
     CDS             complement(809..1351)
                     /locus_tag="AC16JC_14435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165944.1"
                     /translation="MRPAVCVCLAIGGVAALCASPVTGQVGELHLGVIASYGTPETYG
                     PGAGVVLGIAPGRLVYVGVRWSYQIGATLQQVRNRVQTYAVDLGVQFPVGSLEVVPSM
                     SLGVLRFAQREAGISRHSTEFLAAPGMSVEIPVARIALIPEIQYYLAGSPALLHPVTH
                     RGVLASMRLVVPIEVSRFKW"
     gene            complement(1348..>2118)
                     /locus_tag="AC16JC_14440"
     CDS             complement(1348..>2118)
                     /locus_tag="AC16JC_14440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165945.1"
                     /translation="TNAPSNGFPQMSRARELLTRLLIVRHVLATEIAASGGDLRTGLY
                     ALPSDQHGFASRAERWARQIDTTPMLRALFPFAPALLPMVADVGRGTLDSGSAAYDPA
                     ADTRRPKLHQKVQFLATGAFWRAITSSPEWPRFMAAYLRYRESTYSRQATDGSDRGLS
                     DSLEAIAERLLAPVRDTPRAASFAMLGSQNQDPRGMFMDGEVGVLLTGPESLMPLIDI
                     VFMAGTVTWVDDQATLDRMIPPVGELRRRIARIAKDGV"
BASE COUNT          355 a          746 c          744 g          273 t
ORIGIN      
        1 gcggcgccga gtcattggtc acgagaatcg cagcctcgcg aatcaccgcc gcggattcgc
       61 gcagcgacaa ccggccgcac aggctcacga cgcggtgcgc ggcgtctccc tcgggtgccg
      121 cggccacgat cgcctcggcg agcgccgcat cgtccggtcc gcccaccagc acgacgtcca
      181 attgccgcgc cagccgccgc gccagcgcgg cgaaatgcgg ccaccgcttg ctgccccaga
      241 tggagcccgg agcgagagcc gcgaagggcg gcgcgacggc cgccgacctg atcgctgctt
      301 cggcccgggc cacgtcgttc gctgcgagct gcagcgtggg ccgggcccca tggtgcggcg
      361 tcgcgtcgac caacgccagc agccggtcga cctcgtgccc gtctcgtggg cggggccgcg
      421 cttcggtgta cagcgagcgc caaccatccg agtagccgac gcggcgcggg atgcgggcga
      481 gccgcgccaa cgcggcgctg cgcagggagc ggtgaggaag gtaagccacc gcatagcgtt
      541 ccgcacgcag ccggcgggcc agccgccgca ggcccgcgag cccacgatcg gccttccgct
      601 tgtcgtaaac gagcacctgc cgcaccgccg gatgcgtttc gagcagcggc gcggcggccg
      661 gcgtggtcac tacgtccacc ggtccgtgcc gctcggcgag cacgacgagc agcggagtag
      721 tgaggacgac atcacccagg aatgccgtct ggatgacgag agcaggaacc gggggcggcg
      781 ccgtcatgga gggtggaatc tacgacaact accacttgaa ccggctgacc tcgatcggaa
      841 ccacgagcct catcgaggcg agcacaccgc gatgcgtcac cggatgcagt agagccgggc
      901 tgcccgccag gtagtattga atctccggga tgagagcgat ccgggccacc gggatctcca
      961 cggacatccc cggcgccgcc aggaactcgg tggagtgcct ggaaatcccc gcctcgcgct
     1021 gcgcgaaccg gaggaccccc aggctcatgc ttggcacgac ctcgagcgac ccgacgggga
     1081 actgcacgcc gaggtccacg gcgtaggtct gcacgcggtt gcgcacctgc tgcagcgtgg
     1141 caccgatctg gtacgaccag cggacgccga cgtacacgag acggccgggt gcgatcccga
     1201 gaaccacccc ggccccgggc ccgtaggttt cgggcgtgcc gtagctggcg atgacgccga
     1261 ggtgcagctc accgacctgg cccgtcaccg gagacgcaca cagcgcggcg accccgccga
     1321 tggcaaggca cacgcagaca gcgggtctca cacgccgtcc ttggcgatcc gcgcgatgcg
     1381 gcgtcgcagc tcgccgaccg gggggatcat ccggtcgagc gtcgcctgat cgtccaccca
     1441 cgtgaccgtg ccggccatga acacgatatc gatcagcggc atcagcgact ccgggccggt
     1501 cagcagtacg ccgacctcgc cgtccatgaa catgccgcgg gggtcctggt tctgcgagcc
     1561 gagcatggcg aagctcgcgg cccgcggggt gtcccgaacc ggggccaaca accgctcggc
     1621 gatggcttcc agagaatccg atagtcctcg atccgacccg tccgtggctt gccgtgaata
     1681 ggtggactcg cggtaccgca ggtaggccgc catgaaccgc ggccactccg gggaggacgt
     1741 gatcgcccgc caaaaagccc cggtggcgag gaactgcacc ttctgatgca gcttgggccg
     1801 tcgcgtatcc gcggcgggat cgtaggcggc acttccggaa tcgagggtcc cccgcccaac
     1861 atccgcgacc atcggcagga gcgccggtgc gaacgggaac agcgcgcgga gcatgggcgt
     1921 ggtgtcgatc tggcgggccc agcgctcggc gcggctcgcg aatccgtgct ggtcggacgg
     1981 gagcgcatag agtcccgtgc gcaggtcgcc cccggaagcc gcaatctccg tggccaacac
     2041 gtggcgcacg atcaagagcc gcgtcaggag ctcgcgtgcc cgggacatct gggggaagcc
     2101 gttgctcggt gcgttggt
//
LOCUS       DBNGZW010000613         2583 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_194512, whole genome shotgun sequence.
ACCESSION   DBNGZW010000613 DBNGZW010000000
VERSION     DBNGZW010000613.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_194512"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..803)
                     /locus_tag="AC16JC_14445"
     CDS             complement(57..803)
                     /locus_tag="AC16JC_14445"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008100143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR5165946.1"
                     /translation="MRTLLTVAVVSLALGSAPVEGRRSHHTYVIVHGAWGGGWDWRAV
                     DSMLTQRGHRVVRVTLTGVGERVHLASPEVGLSTHIADVVNTILWEDLHDVVLVGHSY
                     GGMVITGVADRIPDRIRRVVYIDAMLPDSGETVLTLMAASGLEFIRSTVRNGMSVPPW
                     VTDTLAIPRDVPQPYRTFSDTLALVNPAGRRVPGTYVLTVQAAQSPDPFQWAADRAAA
                     RGWPVHRLDADHTPERSAREPLVALLDGVP"
     gene            947..1897
                     /gene="gnd"
                     /locus_tag="AC16JC_14450"
     CDS             947..1897
                     /gene="gnd"
                     /locus_tag="AC16JC_14450"
                     /EC_number="1.1.1.343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008923777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase
                     (NAD(+)-dependent, decarboxylating)"
                     /protein_id="HIR5165947.1"
                     /translation="MQLGMIGLGRMGANMVRRLLRNGHQCVVHDLNPAAVEGLVQEGA
                     SGAGALDQLVAQLDPPRTVWLMVPAAAVDGALNDLVPRLARDDVVIDGGNSHYVDDIR
                     RARELEARGIQYLDVGTSGGVFGLERGYCLMIGGPEGVARRLDPIFRTLAPGRGTVAP
                     TPGRGQGGKGATAEEGYLHCGPAGAGHFVKMIHNGIEYGLMAAYAEGINILRHARYDF
                     ALPDIAEVWRRGSVVSSWLLDLAARALVRQPDLARFSGRVPDSGEGRWTIATAIEEGV
                     PAPVLSAALYERFTSRGEGDFAAKLLSALRFEFGGHEEKP"
     gene            1905..2405
                     /locus_tag="AC16JC_14455"
     CDS             1905..2405
                     /locus_tag="AC16JC_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013600811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - obsolete D-gluconate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HIR5165948.1"
                     /translation="MSPRLCVMGVSGAGKSVVGALLAAALGVPFLEGDDLHPADNVRR
                     MAQGIPLTDDDRRGWLTEIAARLTAARRSGAGLVVSCSALKRAYRDVLRRADPEIRFV
                     HLTGDPALISRRLSQRVGHYMPATLLDSQLATLELPAPDEDAWTFDLADSPDSLVSQI
                     VARLNK"
BASE COUNT          391 a          893 c          903 g          396 t
ORIGIN      
        1 catgggcgct cgcaaccaag gtacggcacc gtcaagggcg ccggattaag gcagcttcat
       61 ggcacgccgt ccagcagagc gacgagcggc tcccgtgccg agcgttcggg cgtatggtcc
      121 gcgtcgagcc gatgtacggg ccacccccgg gccgcagccc ggtccgcggc ccactggaac
      181 ggatccgggc tctgcgccgc ctggaccgtc aggacgtagg tcccgggcac ccgtctcccc
      241 gccgggttga cgagcgcgag cgtgtcgctg aaggtgcggt acggctgcgg cacgtcgcgc
      301 gggatggcga gcgtatcggt gacccacggc ggcacgctca tgccgttgcg caccgtggaa
      361 cggatgaact ccagtccgct ggccgccatc agcgtcagca ccgtttcccc ggaatcgggc
      421 agcatcgcgt cgatgtagac gacgcggcga atccggtccg ggatgcgatc cgcgacgcca
      481 gtgatcacca tcccgccgta gctgtgcccc accagcacga cgtcgtgcag atcttcccac
      541 aggatggtgt tcaccacgtc ggcgatgtgg gtggacaagc cgacctccgg ggacgcgagg
      601 tgcacgcgct cgcccacgcc cgtgagcgtg acgcggacca cgcggtgacc ccgctgcgtc
      661 agcatcgagt ccacggcgcg ccagtcccac ccgccgcccc aggcgccgtg cacgatgacg
      721 taggtgtggt gtgagcgccg gccctcgacc ggcgcgctgc cgagagcgag tgagacgacc
      781 gcgacggtga gtagtgtccg catggtctgt ccgggtcctc ctgtgcagct ggcgctggtg
      841 ccgggtcgcg gtagcgcaat aacgtgcgga gcgccgtcgc cggaagcaag ggacgcggct
      901 accttgggcc gacggatcag tcctgacctg gcgaaaggac ccggacatgc agctcggaat
      961 gatcggcctg gggcggatgg gcgccaacat ggtgcgacgc ctgctgcgca atgggcacca
     1021 gtgcgtcgtc cacgacctga acccggcggc ggtggagggt ctcgtgcagg agggggcgtc
     1081 cggagccggg gcgctcgacc agctggtcgc ccagctcgat cccccgcgca ccgtctggtt
     1141 gatggttccg gcggccgcgg tggacggcgc gctgaacgac ctcgtgccgc ggctcgcgcg
     1201 ggacgacgtc gtcatcgacg gcggtaactc acactacgtg gacgacatcc ggcgcgcccg
     1261 ggagctggag gcgcgcggca ttcagtacct cgatgtcggc acgagcggcg gcgtcttcgg
     1321 gctcgagcgc ggctactgcc tgatgatcgg cggcccggaa ggagtggcgc ggcgactcga
     1381 tcccatcttc cgaaccctcg ctcccggccg cgggacggtc gccccgacac cgggacgcgg
     1441 gcaaggaggc aagggagcga cggcggaaga ggggtatctc cactgcggac ccgccggcgc
     1501 cgggcatttc gtgaagatga tccacaacgg catcgagtac ggcctcatgg cggcctacgc
     1561 cgaagggatc aatatcctgc gccacgccag gtacgacttc gccctccccg acatcgccga
     1621 agtgtggcgc cgcggcagcg tggtgtcgtc ctggctgctc gatctcgcgg cgcgcgccct
     1681 cgtccggcag ccggacctcg cgcgattctc gggacgcgtg cccgactcgg gtgaagggcg
     1741 ctggaccatc gcgaccgcca tcgaggaggg ggtgccggcg ccggtgctct ccgccgcgct
     1801 gtacgagcgc ttcacgtcgc ggggcgaggg agacttcgcc gccaagctgc tctcggcgct
     1861 gcgtttcgag ttcggcggcc acgaggagaa gccgtgagcg ccgtgtgtcg ccgcgtctct
     1921 gcgtgatggg agtttccggc gccggcaaga gcgtggtcgg tgccctgctg gccgccgccc
     1981 tcggcgtgcc gtttctggaa ggcgacgacc tccaccccgc cgataacgtg cgacgcatgg
     2041 cgcaggggat tcccctcacc gacgacgatc gccgcggctg gctgacggag atcgccgcgc
     2101 gcctcaccgc agcgaggcgg tcgggcgccg gactcgtggt ctcgtgctcc gcgctgaaac
     2161 gcgcctatcg cgacgtgctg cgccgcgcgg accccgagat ccggttcgtg cacctgacgg
     2221 gagatcccgc gctcatctcc agacggctgt cgcaacgggt cgggcactac atgccggcga
     2281 ccctcctcga cagccagctc gcgaccctcg agcttcccgc cccggacgag gacgcgtgga
     2341 ccttcgatct ggccgactct cccgactcgc tcgtgtccca gatcgtggcc cggctgaaca
     2401 aatgagagcc cagtcagtcc gtcactgttt caactgatag aacaacttgt catacgtcat
     2461 gccgcctcgt tcgatgggcg agcgcagcaa gactcccaat ccaaatgtgg tgtgccctgt
     2521 atccgtcggg ctgttgcgct cggttcgcat catgaagacg ccttcgaacc tctccgttga
     2581 cgc
//
LOCUS       DBNGZW010000614         4151 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_112258, whole genome shotgun sequence.
ACCESSION   DBNGZW010000614 DBNGZW010000000
VERSION     DBNGZW010000614.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4151
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_112258"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1962
                     /locus_tag="AC16JC_14460"
     CDS             <1..1962
                     /locus_tag="AC16JC_14460"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3458 domain-containing protein"
                     /protein_id="HIR5165949.1"
                     /translation="PTYDFPNDKMTWSLEATVPGGFTAVSNGTLMVDRRNADGTRTMS
                     WRQGTPSATYLVSLIVAPLVKIRDMWRGIPVDYYVYREDSARAPRLFHRTPDMIDVYS
                     SLTGLRYPWAKYAQTTVADFFGGMENVSATTLVDWLPDTRAYADRPWYDWILIPHELA
                     HQWFGDYVTTENWANMWLNEGFAEFMPGQYWEKRLGRHAADDYYLDEYQQFMQIDARR
                     RMPLAADGSNNIYPKGALVLRMLQRVLGPERFWASIHRYLTDHAYDNATTDDLRQAVL
                     DATGENLDWFWDEWMYQAGYPELTVTAAYDSTASRLTLQVKQTQVDTATADSTGLRVV
                     TPAVFRMPLTIRVGTRSGDVVQQAELSQREQTVVIPGVKSAPTMVIFDDGNAVLKTLN
                     FEQPTAWLANQLDRDPDLWNRHWVIEHLAQRRADSTAAAALARAAVSADYFLTRAQAA
                     EALDSAPVAIALPALERALRDTSAQVRAAAVTPLATMGGPRALDLARAAWSRDTSYEV
                     RARAVQTLARLDSAGRREVIARGLATPSYRDAIANGALGAIIQANDTSFIPQVDSAAG
                     NAIEPSFVLAILGARGSTRALDLLMSHLDDDRAAVRRWALVAIENALPDSLATARLQS
                     VRGRLSHAETRRAVTEALDRLSKQGGHQP"
     gene            complement(1979..2746)
                     /locus_tag="AC16JC_14465"
     CDS             complement(1979..2746)
                     /locus_tag="AC16JC_14465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165950.1"
                     /translation="MRASLSVFILIAALPLPARLVAQDPFEIEVYGYRTAARAEWELD
                     AHLNYIARGSTAFDGSVAPSEHQGHLALELTRGLTDHVEVAAYLLSAYRPGAGVEYAG
                     ARVRSRVRAPETWRLPVGLSLAAELEFSPAAYDENAVGLEIRPIVEKRVGRLLLDADP
                     VLERGLRAHAGARLGEWELEPSARLGFTVSRTLDLALEYYGKLGETAAERVHQIYPGI
                     DLKLGEDFEINLGIGFGLTSSGSRMVLKSRFEIPLDR"
     gene            complement(2823..>4151)
                     /locus_tag="AC16JC_14470"
     CDS             complement(2823..>4151)
                     /locus_tag="AC16JC_14470"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR5165951.1"
                     /translation="EWGKATARFTHYGGEFHLLEADTGSLPPPPPVGGKEEGPVRKLA
                     DDRLQLGGTIPAGRLRLEAKGQWQRHWLAETVEGDTTSAEQGFDLLLNTTSLDLLAHH
                     GAGARVSGTVGTSAYYQSSATRGDEPLVPGARAEGAAAFAFEQVTLGPRWSALAGGRV
                     DLRRLDADSNTDLGLSDQRRDYTAWSGDVGLVFRPAAEIAVAANVGRAWRAPTLFELF
                     TNGPHPGEARFEIGQADLVPESGLNFDFSVRWQAEPVRGEVAGFSNAIDHYIYIAPTG
                     TRTCPDPAIVCAPQDSLSVYRYAQADARLVGGEASLEVAAGSAIALSARVDYVRGERR
                     VNQEPLPQIPPLRAKLGAEWRHGTSGAGLDVDLVACQNRLSRFDQGTAGYGLLDLFGS
                     VAPRLGPRAFRVEVQVRNALNTRYRDFLSRYKAFALNPGRNLVLRVGTEL"
BASE COUNT          663 a         1489 c         1398 g          601 t
ORIGIN      
        1 cccacctacg acttccccaa cgacaagatg acctggtcgc tggaggcgac cgtcccgggt
       61 gggttcacgg ccgtttccaa cggcaccctc atggtcgacc ggcgcaacgc cgacggcacc
      121 cgcaccatga gctggcgcca gggcacgccc agcgcgacct acctggtgtc gctgatcgtc
      181 gcgccgctcg tgaagatccg cgacatgtgg cgcggcatcc cggtggacta ctacgtctac
      241 agggaagaca gcgcccgggc gccacgcctg ttccaccgca cgcccgacat gatcgacgtg
      301 tactcctcgc tcaccggcct gcgctacccg tgggccaagt atgcgcagac gaccgtggcg
      361 gatttcttcg gcggcatgga aaacgtcagc gcgacgacgc tcgtggactg gctgccggac
      421 acgcgtgcct atgccgaccg gccgtggtac gactggatcc tcattcccca cgagctggcg
      481 caccagtggt tcggagacta cgtgaccacc gagaactggg ccaacatgtg gctcaacgag
      541 gggttcgcgg agttcatgcc gggtcagtac tgggaaaagc gcctcggccg tcacgccgcc
      601 gacgattatt acctggacga gtaccagcaa ttcatgcaga tcgacgcccg gcgccgcatg
      661 ccgctcgccg cggacgggtc gaacaacatc tatccgaagg gcgccctggt gctgcgcatg
      721 ctgcagcgcg tcctcggacc cgagcgcttc tgggcctcga tccaccgcta tctcaccgac
      781 cacgcctacg acaacgccac caccgacgac ctgcgccagg cggtgctcga tgccacgggc
      841 gagaacctcg actggttctg ggacgagtgg atgtaccagg ccgggtatcc tgagctcacc
      901 gtcaccgccg cctacgattc caccgcctcc cgcctgacgt tgcaggtgaa gcagacccag
      961 gtcgacacgg ccacggccga cagtacggga ctgcgcgtcg tcacgccggc ggtgttccgc
     1021 atgccgctca cgatccgggt cggcacccgg tccggcgacg tggtgcagca ggccgagctg
     1081 agccagcgcg aacaaacggt cgtgatcccg ggcgtaaaga gcgccccgac gatggtgatc
     1141 ttcgacgacg gcaacgcggt cctcaaaacg ctgaatttcg agcagcccac ggcgtggctc
     1201 gcgaaccagc tcgaccggga tccggacctg tggaaccggc actgggtgat cgagcatctg
     1261 gcgcagcggc gggccgattc cacggccgcc gcggcgctgg cccgggcggc ggtctcggcg
     1321 gactacttcc tcacccgcgc acaggcggcc gaggcactcg acagcgcgcc ggtcgcgatc
     1381 gccctgccgg cactcgagcg cgcgctgcgg gatacctcgg cacaggtccg cgccgcggcc
     1441 gtcaccccac tggcgacgat gggaggcccg cgcgccctcg acctggcgcg ggccgcgtgg
     1501 agtcgcgaca ccagctacga agtccgggcg cgcgcggtac agacactggc gcggctggac
     1561 agcgccggcc gccgcgaggt catcgcgcgg ggcctggcca cgccttccta ccgggacgcc
     1621 atcgcgaacg gcgcgctcgg cgccatcatc caggcgaacg acacgagctt cattccccag
     1681 gtcgacagcg cggcgggaaa cgccatcgag ccgtcgttcg tgctcgcgat actcggcgct
     1741 cgcggcagca cgcgcgcact cgatctgctg atgagccatc tggacgacga ccgcgccgcc
     1801 gtacggcgct gggccctggt cgcgatcgag aacgcgctcc ccgacagcct cgcgaccgcc
     1861 cggctgcagt cagtgcgcgg ccggctcagc cacgccgaga cacggcgggc ggtgacggag
     1921 gccctcgatc gcctgtcgaa acagggaggt caccagccgt agccggagcg gggagcgctc
     1981 agcgatcgag cgggatctcg aagcgcgact tcagcaccat ccggctgccg ctgctcgtca
     2041 agccgaaccc gatgccgagg ttgatctcga agtcctcgcc gagcttgagg tcaatgccgg
     2101 ggtagatctg gtggactcgc tcggccgctg tctccccgag tttcccgtag tactcgaggg
     2161 cgagatcgag cgtgcggctc accgtgaatc ccaggcgggc gctgggctcg agctcccatt
     2221 ccccaaggcg cgcaccggcg tgggcccgca atccccgctc gaggacggga tcggcgtcga
     2281 gcagcaggcg gcccacgcgc ttttccacga tgggacggat ctcgagaccg acggcattct
     2341 cgtcgtacgc ggcgggcgag aactcgagct ccgccgcgag gctcaagccg acgggcagcc
     2401 gccaggtctc cggcgcgcgc acgcgggacc gcacgcgtgc gcccgcatac tcgacgcctg
     2461 ctccgggccg ataggcggag agcagatatg ccgccacttc cacgtggtcg gtcaaaccac
     2521 gcgtcagctc cagggccagg tggccctgat gctcgctggg agcgacgctc ccgtcgaacg
     2581 ccgtcgagcc ccgggcgatg tagttgaggt gagcgtcgag ctcccattcc gctcgggccg
     2641 cggtccgata gccgtagacc tcgatctcga acgggtcctg agcgacgagc cgcgccggca
     2701 gaggcagcgc ggcgatgagg atgaagacag acagcgaagc tctcatggcc cttccatgtt
     2761 agttgagaat cgttctcagt agcacgcaga gcgaaacata caccgtccac cccggcggtg
     2821 ctctacagct cggtcccgac gcgcagcacg aggttgcgcc ccgggttgag ggcgaaggcc
     2881 ttgtagcggc tgaggaaatc ccggtagcgc gtgttgagcg cgttccggac ctgcacttcc
     2941 acccggaacg cccgggggcc caaccggggc gcgacactgc cgaacagatc gagcagcccg
     3001 tagccggccg tgccctggtc gaaccggctg agccggttct ggcacgccac gagatccacg
     3061 tcgaggccgg cgccgctggt gccgtggcgc cattcggctc ccagcttcgc tcgcaggggc
     3121 gggatctgcg gcagcggctc ttgattgacg cgccgctccc cgcgcacgta gtccacgcga
     3181 gccgacagcg cgatggcgct ccccgctgcc acttcgagcg acgcttcgcc gcccaccagc
     3241 cgcgcatcgg cctgcgcgta ccggtacacg gagagcgaat cctgcggcgc gcacacgatc
     3301 gccggatcgg gacaggtcct ggtccccgtc ggggcgatgt agatgtagtg atcgatggcg
     3361 ttggagaacc cggccacctc gccgcggacg ggttccgcct gccagcggac gctgaagtcg
     3421 aagttgaggc cggattcggg taccaggtcg gcctgcccga tctcgaagcg ggcctcgccc
     3481 gggtgcggac cgttggtgaa cagctcgaac agggtcgggg cgcgccacgc ccgccccacg
     3541 ttcgctgcca cggcgatctc cgccgcggga cggaacacca gtcccacatc gcccgaccag
     3601 gcggtgtagt cccgccgctg gtcgctgagc cccagatcgg tgttcgagtc ggcgtccagg
     3661 cggcgcaggt ccacccgccc accggccagc gcgctccagc gcgggcccag cgtcacctgc
     3721 tcgaaggcga acgccgcggc gccttcggcc cgggcgccag gcacgagggg ctcgtcgccg
     3781 cgtgtggcgc tcgactggta gtacgccgat gtcccgaccg tgccggacac ccgtgcgccg
     3841 gcgccgtgat gggcgagcag atcgaggctc gtcgtgttga gcagcaggtc gaacccctgt
     3901 tcagccgagg tggtgtcgcc ctcgaccgtt tcggccagcc agtgccgttg ccactggccc
     3961 ttcgcttcca accgcagtcg ccctgcggga atggtgcccc ccaactgcag tctgtcgtcg
     4021 gcgagcttgc ggacgggccc ctcttccttg ccgcccaccg gcggcggtgg cggcagtgag
     4081 cccgtgtcgg cctcgagcag atggaactcg ccgccgtagt gggtgaatcg cgccgtggcc
     4141 ttgccccact c
//
LOCUS       DBNGZW010000615         3724 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1219449, whole genome shotgun sequence.
ACCESSION   DBNGZW010000615 DBNGZW010000000
VERSION     DBNGZW010000615.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3724)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3724)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3724
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1219449"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1117
                     /locus_tag="AC16JC_14475"
     CDS             <1..1117
                     /locus_tag="AC16JC_14475"
                     /inference="COORDINATES: protein motif:HMM:NF014731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HIR5165952.1"
                     /translation="GPALLLNYFGQGALLLRDPSAAVNPFYHLAPTWFLYPLIALATV
                     AAIIASQAIISGAFSLTRQAVQLGYSPRLQIEHTSSREIGKIYVPAVNWGIMLLTCAF
                     VAGFGSSTSIAGAYGVALSTLMVMTTLMFYVMCREVWRWSFGRAAFVVGLFLAMDVPF
                     LIANGFKIRHGGWVPLVTAAGIFLLMSTWKRGREILSRRLQEKSVPLTILMGDLAADP
                     PLRVPGVAVFMSGNPGGTPPALVHNLAHNKVLHEKVVFLTVVTEDVPHLAPAERVTVK
                     RMGKGFQEVIARYGFMEDPSILDIIDQCRLKGLMIPLEGTTFFMGREELVSTDRPGMA
                     RWRERLFAFMSRNALRATAFFQIPSTQVFEVGAQVEL"
     gene            complement(1114..2061)
                     /locus_tag="AC16JC_14480"
     CDS             complement(1114..2061)
                     /locus_tag="AC16JC_14480"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HIR5165953.1"
                     /translation="MKRWIWGLIVVAGLAAALRLMMRFPWPETVAALGAMHPLLLLAA
                     LVVNLLSPLAKGQAWHLLLRPVARHRWRAAQEATLVGSAVNSISMGVTGDAARVTLLA
                     RREAVPVSTAVLSVAWTRVVEGLGLALFLVLAPLFLHVPATVRGLQLGAAIALVTVFA
                     LSRLGRWARLIVRLPRVLRQPATLLARMSVGTRLAAPVLCALVNWVVQWATYDLVLRA
                     AGLALPVSASLVALLAVNIGGVGRLTPGNLGVTQAAVVAALLPFHVPAERAVAAGLAL
                     QAIQVLPILGLAAALVGWSGLRRLMTASAEEKQALRAVA"
     gene            complement(2061..2780)
                     /locus_tag="AC16JC_14485"
     CDS             complement(2061..2780)
                     /locus_tag="AC16JC_14485"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR5165954.1"
                     /translation="MILPFVAAGASVGAAVGTLAWGAYSPTSPLFGAVIGRGSTTGRA
                     LYLTFDDGPRPGVTDRILETLAREGVPATFFLVGAAVRRHPATAHRVAHAGHEIGNHT
                     RSHRKLHRLGREAVRAEIVGGHQDIVEVTGEVPRAFRAPHGYRNPFVSRVTRRLRYRT
                     FGWTFGVWDSDRPPAEEIRRRVRAKLRPGAIILLHDGDGYDPEGAHPRGDRTPTAEAL
                     PGIIADARAAGYAFHLLAELY"
     gene            complement(2777..>3724)
                     /locus_tag="AC16JC_14490"
     CDS             complement(2777..>3724)
                     /locus_tag="AC16JC_14490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5165955.1"
                     /translation="RVGTSRVIYSNGAFARVTTGWRLTDRIEALLRETGAELVHVQDG
                     LAPVLGPLAANAAGRLGLPVVATFHSWFPRSIGYSLFRRPLQRRLDRLAACVAVSAPV
                     VQALSRYLRGTWDIIPNGVDVDVFHPNGRRPTDYLTRGPRLLFLGRLDPRNGLETVLD
                     AMPRILEHFPRTTLTVAGDGPLRRVYERAARPLGAAVQFVGRVNGERPEYYGTADLYL
                     CPTTKASFGITLLEAMACGTPLVASDIIGFRELVDGGGEAVLVPPRRPDAWADAVAGL
                     IGDPSRRAAMGAAGRTKAGTYAWPRVTDRILEVYRRVLA"
BASE COUNT          595 a         1316 c         1291 g          522 t
ORIGIN      
        1 ggggccggcg ttgctgctca attacttcgg gcagggggcg ttgctgctgc gcgatccgag
       61 cgctgcggtg aacccgttct accacctcgc gccgacctgg tttctctatc cgctgatcgc
      121 cctcgccacc gtcgcggcga tcatcgcgtc gcaagccatc atctcgggtg cattctcgct
      181 cacgcgccag gcggtacagc tcggctacag cccgcgcctc cagatcgagc acacctcctc
      241 acgtgagatc ggcaagatct acgtcccggc ggtcaactgg ggcatcatgt tgctcacgtg
      301 cgccttcgtc gccgggttcg gatcgtccac cagcattgcc ggggcctacg gggtcgcgct
      361 gtcgacgctg atggtcatga cgacgctgat gttctacgtc atgtgccgcg aggtctggcg
      421 ctggagcttc ggccgcgccg ccttcgtggt ggggctgttc ctggcgatgg acgtcccctt
      481 cctcatcgcc aacgggttca agatccggca tggcggctgg gttcccctgg tgaccgccgc
      541 ggggatcttc ctgctcatgt ccacctggaa gagaggccgg gagatactgt cgcggcggtt
      601 gcaggagaag tcggtgccgc tcaccatcct catgggcgac ttggcggccg atccgccgct
      661 gcgcgtcccc ggcgttgcgg tgttcatgtc cggcaacccc ggcggcacac cgcccgcgct
      721 cgtgcacaac ctcgcccaca acaaggtgct gcacgagaag gtcgtgttcc tgacggtggt
      781 caccgaggac gtcccgcatc tcgcaccggc cgagcgcgtc accgtcaagc ggatggggaa
      841 ggggttccag gaagtcatcg cgcgctacgg attcatggag gatccgagca tcctcgatat
      901 catcgaccag tgtcgtctga aggggctgat gattccgctc gaagggacga cgttcttcat
      961 ggggcgcgag gagctggtgt ccacggaccg cccggggatg gcgcggtggc gcgagcggct
     1021 gttcgccttc atgtcgcgca acgcgctgcg cgcgacggcg ttcttccaga tcccctcgac
     1081 ccaggtgttc gaggtgggcg cgcaggtcga gctttaggcg accgcccgca gcgcctgttt
     1141 ctcctccgcc gaggcggtca tcaatcggcg caggccggac caccccacca gggcggccgc
     1201 gaggccgagg atcggcaaca cctggatggc ctggagggca aggcccgcgg ccaccgcccg
     1261 ctcggctggc acgtgaaagg gcagcagcgc cgcgaccacc gccgcctgcg tcaccccgag
     1321 attgcccggt gtgagccggc cgacaccgcc gatgttcacc gccagcagcg ccacgaggga
     1381 ggccgacacg ggaagcgcca gtcccgccgc ccgcagcacg agatcatagg tcgcccactg
     1441 caccacccag ttgaccagcg cgcacagcac cggcgccgcg aggcgtgtcc cgacgctcat
     1501 gcgtgccagc agcgtcgcgg gctggcgcag cacgcgcggc aggcgcacga tcaggcgcgc
     1561 ccagcggccg agccgggaga gggcgaagac ggtcaccagg gcgatggcgg cgccgagctg
     1621 caggccgcgc accgtcgccg ggacgtgcag gaacaacgga gccaggacca gaaacagcgc
     1681 gagccccaat ccttccacca cgcgggtcca ggccacggag agcaccgcgg tgctcaccgg
     1741 aacggcctcc cgccgggcca gcaacgtcac ccgcgcggcg tctccggtca cgcccatgct
     1801 gatcgagttg accgcggagc cgacgagcgt ggcctcctgc gcggcgcgcc agcgatgccg
     1861 ggcgaccggg cgcagcaaca ggtgccacgc ctggcccttg gcgagcggtg acagcaggtt
     1921 gacgacgagc gccgccagga gcagcagcgg atgcatcgct cccagggccg ccaccgtctc
     1981 gggccacgga aaccgcatca tcaggcgcag cgccgcggcg agacctgcga cgacgatcag
     2041 cccccagatc caccgcttca tcaatacagc tccgcgagga gatggaaagc gtagcccgcc
     2101 gcccgggcat cggcaatgat ccccggaagc gcctccgccg tgggcgtacg gtccccacgg
     2161 ggatgggccc cttcggggtc gtagccgtcc ccgtcgtgca gcaagatgat cgcgcccggc
     2221 ctgagctttg cccgcacgcg acgccggatc tcctcggccg gcggccgatc ggagtcccac
     2281 acaccgaagg tccacccgaa ggtacggtag cgcaggcgac gtgtgacccg gctgacgaac
     2341 gggttgcgat acccgtgcgg ggcacggaag gcgcgcggga cttcccccgt gacctccacg
     2401 atatcctggt gtccccccac gatctccgcg cggaccgcct cccgtcccag gcggtgcaac
     2461 ttgcgatgcg agcgcgtgtg gttgccgatc tcgtgaccgg cgtgcgccac ccggtgcgcg
     2521 gtcgccgggt ggcgtcgcac cgcggcgccg accaggaaga aagtggccgg cacgccctct
     2581 cgcgcgagtg tctcgagaat ccggtccgtc acgccggggc gcggcccgtc gtcgaaggtg
     2641 aggtagagcg cacggccggt cgtcgatccg cgaccgatta cggcgccgaa gagcggactg
     2701 gtcggagaat acgcccccca ggcgagcgtc ccgacggccg ctccgacgga tgcgccggcg
     2761 gccacgaacg gcaggatcac gccagcaccc gccggtagac ctcgaggatc cggtccgtca
     2821 cgcgcggcca ggcatacgtg cccgccttgg tgcgtcccgc cgcgcccatc gccgcgcgcc
     2881 gggacggatc accgatcagg ccggccactg cgtcggccca ggcgtccggc ctgcggggtg
     2941 gcacgaggac cgcttcgccg ccgccgtcca ccagctcccg gaacccgatg atgtccgaag
     3001 cgacgagtgg ggttccgcaa gccatcgctt cgagcagcgt aattccgaag gaggccttgg
     3061 tggtggggca gagatagaga tcggcggtgc cgtagtattc cggccgttcg ccgttcacgc
     3121 gcccgacgaa ctgcacggcg gcaccgagcg gccgggcggc gcgctcgtac acgcggcgca
     3181 acggcccatc gcccgcgacc gtcagcgtcg ttctcggaaa gtgctccaga atgcgcggca
     3241 tggcgtcgag cacggtctcg aggccgttgc gcggatcgag ccgaccgaga aacaggagac
     3301 gcgggcctct ggtcaagtag tcggtcgggc ggcgcccgtt gggatggaac acgtctacgt
     3361 cgacgccgtt cgggataatg tcccaggtgc cccgcaaata acgggagagc gcctgaacga
     3421 cgggcgcgct taccgctacg cacgccgcga gccggtccag ccggcgctgc aacgggcggc
     3481 ggaacagact gtacccgatc gagcgtggaa accagctgtg gaacgtggcg acgaccggga
     3541 gtcccagccg cccggcggcg ttggccgcga gggggccgag caccggagcg agcccgtcct
     3601 ggacgtgcac cagctcggcg ccggtctcgc gcaacaacgc ttcgatccgg tcggtgagtc
     3661 gccacccggt ggtgacgcgg gcaaaggcgc cgttggagta gatcacccgg ctcgttccga
     3721 cacg
//
LOCUS       DBNGZW010000616         2880 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1558321, whole genome shotgun sequence.
ACCESSION   DBNGZW010000616 DBNGZW010000000
VERSION     DBNGZW010000616.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2880)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2880)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2880
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1558321"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..598
                     /locus_tag="AC16JC_14495"
     CDS             71..598
                     /locus_tag="AC16JC_14495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165956.1"
                     /translation="MNPARLALLAVAAMAGPLASQGEARTAAWLTLAAGAGYLGGAGS
                     IGVYGAYNYQHEASLFTLRGAGVVDVLTALLSGFGNTSENTGASDIGLLYGRASRPGH
                     GLFALSAGVGLAQVTRDSSGTSHRSYHLTLPLEAQVAWRPARFIGFMVLGFESLNKGR
                     SFRGITVGVQLGRLY"
     gene            645..1118
                     /locus_tag="AC16JC_14500"
     CDS             645..1118
                     /locus_tag="AC16JC_14500"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR5165957.1"
                     /translation="MRLGVIADTHGLLRPEVFEVFRAVDHILHAGDVGSWELIVELQA
                     LAPVTAVYGNTDGSELRARLPQVASLQLDGFDIVVSHGDQLGSPTPARLHDAFPEAEI
                     VLFGHTHRPLLELVDRTVTVMNPGSAGPRRFELPVTVGILELEPGIPPRGRIVEL"
     gene            complement(1187..2629)
                     /locus_tag="AC16JC_14505"
     CDS             complement(1187..2629)
                     /locus_tag="AC16JC_14505"
                     /inference="COORDINATES: protein motif:HMM:NF017044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR5165958.1"
                     /translation="MRTLTLAGLLFCAALPAAAQRPARDTRAQIQALRYPPLRFTPPV
                     PKRLTLSNGVPVYLLEDHSLPLLDVEVVSKVGVANLPDSLWGVGWQADVLMRTGGTTR
                     LSPDSVDQLVEFYALNVFISTGNEQSTASFSGLSRYRDQMLDLGFDLLRNPRNDTSRV
                     RITAGQQLEAWRRRNDQPGSVLNRAWPQVLLGDHPLGRTQVTPDEVAGFTPARFRWTQ
                     ERLFCPQNLLIGVSGDFDEKTLLAKLESLFRGWPRCAPGLREIPPLQFATGPKIVFVE
                     KDVNQTNIWMGHAGSIRVADTPDYYAAQVADFLLGGGGGFNSRLLQRLRSDSGFAYST
                     FSNWDADTRREGIFFAGAQIRAEKTVAGLSLMRQIIGSMATDPVTARDVQLAKDNEVN
                     SFVFRFQNAGQIVAQQLGYAVDGLPGNWFDLYLKGIQAVTPEQVADVSRRLLHPDRLV
                     MVVVGKSSAFDKPLSTLGPVTMMPVDSIYR"
     gene            complement(2629..>2880)
                     /locus_tag="AC16JC_14510"
     CDS             complement(2629..>2880)
                     /locus_tag="AC16JC_14510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HIR5165959.1"
                     /translation="RVRNQVQASAIQRLQDNFGLAFQLSQSEALWYDWRQTFRDGAAV
                     ARVTADDVQRVARTYFAKSNRTVATLVRPAAADSTGGTR"
BASE COUNT          452 a          921 c         1027 g          480 t
ORIGIN      
        1 tgcagcgcgt tctcgatcat ctcggcatcg agatcgatct ggtgaagcgc tggggaggac
       61 ccgtacaggg atgaatcctg ctcgtctggc gctgctcgcc gtggcggcga tggctggacc
      121 gctcgcctct cagggcgaag cacggaccgc cgcgtggctc accctggccg ctggcgcagg
      181 gtacctgggc ggagccggtt cgattggcgt gtacggagcc tacaactacc agcatgaggc
      241 gagcctcttc acccttcgag gcgccggggt cgtcgacgtg ctcaccgctc tcctgagcgg
      301 cttcgggaac acgagtgaga acaccggggc gagcgacatc gggctgctgt atgggcgggc
      361 gagccgtccc ggtcacgggc tcttcgctct atccgccggc gtcggacttg cccaggtgac
      421 gcgcgactcg agcggtacga gtcaccggag ctaccacctc acgctgcccc tggaggcgca
      481 ggtggcttgg cgtcccgcga ggttcatcgg cttcatggtt ctcggattcg aaagtctgaa
      541 caagggccgg tcgtttcggg gaataacggt cggcgtgcaa ttggggcgcc tctactgaag
      601 gctctcgacg aactcggcga gacattgacc gggcatccct gaacatgcga cttggcgtca
      661 ttgccgacac ccacgggctg ttgcggccgg aggtgttcga agtcttccgc gcggtcgatc
      721 acatcctgca cgccggcgac gtggggagct gggagctgat cgtggaattg caggcgctgg
      781 cgcccgtgac cgccgtctac ggcaacacgg acggctccga gctgcgggca cggttgccgc
      841 aggtcgcgtc gctccagctg gacggattcg acatcgtggt gagccacggg gatcagcttg
      901 gtagcccgac ccccgcccgc ctgcacgacg ccttccccga ggccgagatc gtcctgttcg
      961 gccacacgca caggccgctg ctggagctcg tggaccggac cgtcaccgtc atgaatccgg
     1021 ggagcgcggg cccccggcgg ttcgagcttc ccgtgacggt cggaatcctg gagttggagc
     1081 cggggattcc tccacgtggg agaatcgtcg agctgtgaga gcagggagca cggagcagca
     1141 cgtgtgctct gggcctcctg ctcctggctc tgtgctcctg cgcatctcag cggtagatcg
     1201 aatccacggg catcattgtg accgggccga gcgtcgagag cggcttgtcg aacgcgctgg
     1261 acttgccgac gacgaccatc accagacggt cggggtgcag gaggcggcgc gacacgtccg
     1321 cgacctgctc cggggtcacg gcctggatgc ccttcagata gagatcgaac cagttgccgg
     1381 gcagtccgtc cacggcatag ccgagttgct gcgccacgat ctgccccgca ttctggaacc
     1441 tgaacacgaa gctgttgacc tcgttgtcct tggcgagctg cacgtcgcgc gcggtgaccg
     1501 ggtcggtggc catggacccg atgatctgcc gcatcagtga caacccggcc acggtcttct
     1561 ccgctcgaat ctgggcgccg gcgaagaaga tgccctcccg cctggtgtcg gcgtcccagt
     1621 tcgagaaggt gctgtaggcg aatccggaat cgctgcgcag gcgctgcagc agccgggagt
     1681 tgaagccccc acctccgccg agcaggaagt cggcgacctg ggccgcgtaa tagtccggcg
     1741 tgtcggccac gcggatgctc cctgcgtgtc ccatccagat gttggtctgg ttcacgtcct
     1801 tttcgacgaa gacgatcttc gggccggtcg cgaactggag cgggggaatt tcccgcaacc
     1861 ccggcgcgca ccgcggccag ccgcggaaca ggctctcgag cttggccagg agcgtcttct
     1921 cgtcgaagtc gcccgacacg ccgatcagca ggttctgcgg gcagaagagg cgctcctggg
     1981 tccagcggaa ccgggccgga gtgaaaccgg cgacttcgtc gggggtcacc tgggtccggc
     2041 ccagcgggtg atcgcccagc agcacctgcg gccacgcgcg gttcagcacc gatcccggct
     2101 ggtcgttgcg gcggcgccag gcctcgagct gctggcccgc ggtgatccgt acccgcgacg
     2161 tgtcgttgcg cgggttgcgc aacaggtcga agccgagatc gagcatctgg tcccggtagc
     2221 gcgacaggcc gctgaacgag gcggtgctct gctcgttgcc ggtggagatg aagacattca
     2281 gggcgtagaa ctcgaccagc tggtcgacgg agtcgggact caggcgggtg gtcccgccgg
     2341 tgcgcatcag gacgtcggcc tgccagccga cgccccacaa cgagtcgggc aggttcgcga
     2401 ctcccacctt ggacactacc tcgacgtcga gcagcggcag gctgtggtcc tcgagcaggt
     2461 agacgggaac gccgttcgac agcgtgagcc gtttcggaac cggcggggtg aaccgcagcg
     2521 gcggatagcg gagcgcctgg atctgggccc gggtatcgcg cgcggggcgc tgcgcggcgg
     2581 cggggagtgc ggcgcagaac aggaggccgg cgagagtcag ggtgcgcatc agcgggtccc
     2641 tccggtcgaa tcggccgctg ccggacgcac cagggtcgcg acggtgcggt tggatttcgc
     2701 gaaataggtc cgggcgacac gctgcacgtc gtccgcggtc acgcgggcga cggcggcgcc
     2761 gtcccggaag gtctggcgcc agtcgtacca gagggcttca ctctgggaca gctgaaacgc
     2821 gagcccgaag ttgtcctgga ggcgctggat ggcgctcgcc tgtacctggt tgcgcacgcg
//
LOCUS       DBNGZW010000617         2677 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_943558, whole genome shotgun sequence.
ACCESSION   DBNGZW010000617 DBNGZW010000000
VERSION     DBNGZW010000617.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2677)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2677)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2677
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_943558"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..626)
                     /locus_tag="AC16JC_14515"
     CDS             complement(<1..626)
                     /locus_tag="AC16JC_14515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5165960.1"
                     /translation="MRLALVTEFYYPHLGGVTEHVHHLALELRRRGHDVTVLTSHMKG
                     GRADPPHVRRVGTSRVIYSNGAFARVTTGWRLTDRIEALLRETGAELVHVQDGLAPVL
                     GPFAAGAAGRLGLPVVSTFHSWFPRSIGYSVFRRPLQRRLDRLAACIAVSAPVVQALS
                     RYLRGSWDIIPNGVDVNLFHPNGRRPTDYLTRGPQLLFLGRLDPRNGL"
     gene            complement(1298..1798)
                     /locus_tag="AC16JC_14520"
     CDS             complement(1298..1798)
                     /locus_tag="AC16JC_14520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165961.1"
                     /translation="MLTFVLALAVALQAPDSARAGRWERALNETTDSLDRVRGAAATF
                     RVDLDNASHDLVLVRAAHLRAACSAAGAPLQVLEQLLTNEVYDHSARGAQERLRSGTT
                     ELRRALSRCRREWQAGPRPTTSGADSLRAWGPYRTAQLDAALRRYLGLVRSFMKQASL
                     KKAAVS"
     gene            complement(1800..2426)
                     /locus_tag="AC16JC_14525"
     CDS             complement(1800..2426)
                     /locus_tag="AC16JC_14525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165962.1"
                     /translation="MTVPATTRAWRMFEDRPEALSHFFARAGEAPRFIAFDDTVGCPL
                     DQALAVVEWTEAAGILHDDDVMHAAWIRPDAAAAVVERRDGERRSFAYFGPRMDAPYA
                     DPGECELIYDGPGVRAYAFAERGPALAHFLRATQGTGAILALLARRGPELRHVRRWLK
                     ELFAAPPTAEHRSTQLMAGWFATSGAGCLFLPAQAGEPYSYCEVGTDS"
     gene            2487..>2677
                     /locus_tag="AC16JC_14530"
     CDS             2487..>2677
                     /locus_tag="AC16JC_14530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165963.1"
                     /translation="MRRWVTALIVTGVILALPLSGQDPTPPPPESAATAPPAPIPPSP
                     GQERYLQGLRTAGRGVAQI"
BASE COUNT          444 a          921 c          916 g          396 t
ORIGIN      
        1 tcgaggccgt tgcgcggatc gagccggccg agaaacagga gctgcggacc cctggtcagg
       61 tagtcggtcg gacggcgccc gttgggatgg aacaggttca cgtcgacgcc gttggggatg
      121 atgtcccagg aaccgcgcag gtagcgggaa agcgcctgaa cgacgggcgc actcaccgcg
      181 atgcacgccg cgagccggtc cagccggcgc tgcagcgggc ggcggaacac actgtacccg
      241 atggagcgtg gaaaccagct gtggaacgtg gagacgacgg ggagtcccag ccgcccggcg
      301 gcgccggccg cgaaggggcc gagcaccgga gcgagcccgt cctgcacgtg caccagttcg
      361 gcgcccgtct cgcgcaatag cgcttcgatc cggtcggtga gtcgccaccc ggtggtgacg
      421 cgggcaaagg cgccgttgga gtagatcacc cggctcgtgc ccacgcgacg cacgtgcggc
      481 gggtcggcgc gccccccctt catgtgcgag gtcaggacgg tgacgtcgtg gccgcggcgg
      541 cgcagctcga gcgcgaggtg gtgcacatgc tcggtgaccc cacccaggtg ggggtagtag
      601 aactcggtga cgagggcgag acgcatgggt accggggcgg ccttggaggg gtccccatgt
      661 gaccccgtca tgccccggag cgtcaaaaaa aacggcagga aaatcgaagc ccccggaacg
      721 gctcgatagg ccgcccgggg gccccgcaca ccggttaccc ggagccgggg gattgctccc
      781 tccgttcttc atcctgccgc cgccggttga ctgttgcgtc gctcggtagg gcgatcagga
      841 gctccggctt cgatccccat gcagcctggc cgggccacac ggttcaccgc cgtgagtgct
      901 gcgggtcaga tcgggtcgcg gaccgacctt ccgcactgca cgtcgaggca ctacttcgac
      961 gtggctgagg cacgcgactg gcctcaacga tccgagccgg agagatcctc ccggatcagc
     1021 aggcgcctga gcccttgttg catcggcgcc ctccccggaa tttccgctcg gactgggtcg
     1081 tgcgggcgtt gctgagacac caggccccaa ctcttccccg cgcgacgtgg aacacaatct
     1141 aggggcttgg caaaatcgcg caagtgccga tgtttcttcg ggttagctca ctgcgatgtg
     1201 caccgactgt ggaacctaac cgccatgaaa tatgaccgtt gcgacgtccg tccacacagt
     1261 gtggaattgt gtatccacag ccattccact tcccacatta gctgacagcg gccttcttca
     1321 ggctcgcctg tttcatgaac gagcgcacca gaccgagata gcgccgcagc gccgcatcca
     1381 gctgtgcggt gcgatacggt ccccacgcgc gcaggctgtc ggcgccgctg gtggtgggac
     1441 gcggacccgc ctgccactcc cgccgacagc ggctcaacgc ccggcggagc tcggtagtac
     1501 cgctgcgcag tcgctcttgg gcacccctgg cgctgtgatc gtacacctcg ttggtgagca
     1561 gctgctccag cacctgcagt ggggcgcccg cggcactgca ggcagcccgg aggtgcgcgg
     1621 cccgaaccaa cacgagatcg tgcgaggcgt tgtcgaggtc gactcggaag gtggcagcgg
     1681 cgccgcggac tcgatcgagc gaatcggtcg tttcgttcag ggcgcgctcc cagcgccccg
     1741 cgcgcgcgct gtcgggcgcc tgcagggcca cggcaagcgc aaggacgaac gtcaacacgt
     1801 caggagtcgg tgccgacttc gcagtaggag taaggctccc cggcctgggc cgggaggaag
     1861 agacaaccgg cgccggaggt cgcgaaccat cccgccatga gctgggtcga gcgatgctcc
     1921 gcggtcggcg gcgcggcgaa cagttccttg agccagcgcc gcacgtgccg cagctcgggt
     1981 ccgcgccggg cgagcagcgc cagaatggcc cccgtcccct gggtcgcccg gaggaaatgc
     2041 gccagcgcgg gcccacgctc cgcgaacgcg taggcccgca cccctgggcc gtcgtagatc
     2101 agctcgcatt ccccgggatc ggcatacggc gcgtccatcc gcggtccgaa gtaggcgaac
     2161 gaccgacgct ccccatcgcg gcgttcgacc acggcggccg cggcatcggg ccggatccag
     2221 gcggcgtgca tgacgtcgtc atcatgcagg atcccggcag cctcggtcca ttccaccacg
     2281 gccagggcct gatcgagggg gcatcccacc gtatcgtcga aagcgatgaa tcggggggct
     2341 tcccccgccc gggcgaagaa gtggctcaac gcctctgggc ggtcctcgaa catgcgccat
     2401 gcccgagtgg ttgcgggaac ggtcatgacc agattataac ccatggcctg agcaaagtgg
     2461 ccggactcca gatgcaggaa cctcgcatgc gccgttgggt taccgccctc atcgtcaccg
     2521 gagtgatcct cgcgctgccc ctgtcggggc aggacccgac ccctcctcct ccagagtcgg
     2581 ctgccaccgc tcccccggct ccgatccccc cctcgccggg gcaggaacgc tacctccagg
     2641 gcctgcgcac cgccggtcga ggggtggcgc agatcaa
//
LOCUS       DBNGZW010000618         1757 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_112527, whole genome shotgun sequence.
ACCESSION   DBNGZW010000618 DBNGZW010000000
VERSION     DBNGZW010000618.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1757)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1757)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1757
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_112527"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(78..497)
                     /locus_tag="AC16JC_14535"
     CDS             complement(78..497)
                     /locus_tag="AC16JC_14535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HIR5165964.1"
                     /translation="MKSHTHYLWFNTEQRQEIIDVTEEVAEQVRASGVQEGLVLVSAM
                     HISASVFVNDHESGLWHDILHWLEHSVAPWDPKRYRHNDTGEDNAAAHLRSLTVGHEV
                     IVPITKGKLDFGPWQRVFYGEWDGGRRKRVVIKVLGE"
     gene            619..>1757
                     /locus_tag="AC16JC_14540"
     CDS             619..>1757
                     /locus_tag="AC16JC_14540"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5165965.1"
                     /translation="MALPFRRRIFFVLVLMAAVPTGLAVVSWVLTVRTFGPTAGARAS
                     LEEVAGSARGMLEQLDTTQLAPAARAGVRDHLEAVSRGVSFARRADRYLRYYTAGLAI
                     VVIALGSLVLVAAVYLAGHLSRQLSRPIDELVGWTRLIRRHETLPAGPPTRGAPEFAS
                     LRLALRDMAGALDTARGRELEAERLRAFREVARRVAHEIKNPLTSMRIAVDQLQRADA
                     AGPANGRTAMAREVLAAETDRLERLAREFADFGRLLEGPTSEVDLVELLEELGRTAVP
                     EGVAVHVTPNGDRRTIQGHYDPLRRAFSNLLRNAAEAMAGHGSIDVAVGGEGRGVVIT
                     VADHGPGIPAELRGRVFEPYLTTKPDGTGLGLALVRQTVDAHHGT"
BASE COUNT          249 a          604 c          614 g          290 t
ORIGIN      
        1 cgtcctccgc tcctgttccg gactccatcg ggaaccattg aaattaaaca acttgcgccc
       61 aaataggaag ccgccgctca ctctcccagg actttgatga ccacccgctt gcggcgcccg
      121 ccatcccact ccccatagaa cacccgctgc cacgggccga aatcgagctt ccccttggtg
      181 atcgggacga tgacttcgtg gcccaccgtc aggctgcgca ggtgagcggc ggcgttgtcc
      241 tcccctgtgt cgttgtgccg gtaccgcttc gggtcccagg gggcgacgga gtgctccagc
      301 caatggagga tgtcgtgcca caggccggac tcgtggtcgt tgacgaagac cgacgccgag
      361 atgtgcatcg ccgaaacgag cacgagtccc tcctggacgc cgctcgcgcg cacctgctcg
      421 gcgacctcct cggtgacgtc gatgatctcc tgccgctgct ccgtgttgaa ccagaggtag
      481 tgggtgtgac tcttcatcag ctcctcccgg gagaacagag ccggaagcac aaagcagtac
      541 tgctccctgc tccgtgcgct gtttcggtcc cgtgtcagtt tgtcgcgccc gctccaacct
      601 cctagattgc tgcaagatat ggctcttccg ttccgtcggc gcatcttctt cgtcctggtc
      661 ctgatggcgg ccgtgcccac cgggctggca gtcgtctcct gggtgctgac ggtgcggacc
      721 ttcggaccga cggccggcgc gcgggcatcg ctcgaggagg tggccggctc ggcccggggg
      781 atgctcgaac agctcgatac gacgcagctc gctccggcgg cgcgcgccgg cgtgcgggac
      841 catctggagg cggtgtcacg gggcgtgagc ttcgcgcggc gcgccgaccg ctatctccgc
      901 tactacacgg ccgggctcgc catcgtggtg atcgccctgg ggtcgctcgt gctggtcgcc
      961 gccgtgtacc tggccggtca tctctcacgc cagctgtcgc ggccgatcga cgagctggtg
     1021 gggtggacgc gtctcattcg ccggcatgag acgctgccgg cgggcccgcc cacccgcggc
     1081 gcgccggagt tcgcctcgtt gcggctggcg ctgcgggaca tggcgggcgc cctggacacc
     1141 gcccgtggcc gcgagctcga ggcggagcga ctccgggcat ttcgcgaggt ggcgcggcgc
     1201 gtcgcccatg agatcaagaa tccgctcacc tcgatgcgta tcgccgtgga ccagctccag
     1261 cgcgccgacg cggcgggtcc cgctaacggg cgcacggcga tggcgcggga ggtcctggcc
     1321 gcggaaaccg accggctgga gcggctggcc agggagttcg ccgacttcgg ccgtctcctg
     1381 gaggggccga ccagcgaggt ggacctggtg gagctgctgg aggagctggg ccggacggcg
     1441 gtgcccgaag gggtggcggt gcacgtgacc cccaacggcg accgtcgcac gatccaggga
     1501 cactacgacc cgctgcgccg cgccttcagc aatctgcttc gcaacgcggc cgaggcgatg
     1561 gcgggacacg ggagcatcga cgtggccgtg gggggggagg gtcgcggcgt cgtgatcacc
     1621 gtcgccgacc acgggcccgg catccccgcc gagctgcgcg gccgcgtatt cgagccctat
     1681 ctgacgacca agccggacgg gacgggactc ggtctggcgc tggtgcgcca gacggtggac
     1741 gcccatcacg gaacgat
//
LOCUS       DBNGZW010000619         4337 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1353204, whole genome shotgun sequence.
ACCESSION   DBNGZW010000619 DBNGZW010000000
VERSION     DBNGZW010000619.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4337)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4337)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4337
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1353204"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1249
                     /locus_tag="AC16JC_14545"
     CDS             <1..1249
                     /locus_tag="AC16JC_14545"
                     /inference="COORDINATES: protein motif:HMM:NF023266.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ERAP1-like C-terminal domain-containing protein"
                     /protein_id="HIR5165966.1"
                     /translation="AIDSRLDAFGRSFMLRPGMPVVEPRLTIRNGTIARLEIVQHAAQ
                     PALSGAAPWPMRTQVLLAYRGAQPVRLPIALRGTVTEVPEARGRSAPSWIFPNAGDYG
                     YFLSLLDTASVSALERGGLREVHDGLLRAMLWGALWDQVREARLDPARFARLVLAELP
                     FERDEQIVPALLGRLGRALDAYLAPARARPIGAAAERVLWETAGDTTLAYGIRRAALD
                     AFLGLVTTPEGIADMKTLLHADSAAGGPLRDPTRWSIVNRLLILGDAEAEPALVAQAT
                     RDTTPDGRRRAFVAGAARPSADVKADYFRRWFADASLNEDWASGSFGTFNAIEHQALT
                     LPFLRPALDSLPYIQHHRRIFFLGAWLGSFLGGQSSRAALEVVRAYLADHPDLPADLR
                     QKVLQSADELERTVRIRALWGAQ"
     gene            1314..>4337
                     /locus_tag="AC16JC_14550"
     CDS             1314..>4337
                     /locus_tag="AC16JC_14550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019670322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR5165967.1"
                     /translation="MSRLPSLAVLLVLAAPPARAQQVDTTLWTNLRFRFIGPDGNRAS
                     AIVGEPGNPFVAYVGAASGGVWKTEDGGVHWRAVFDSEPAQAIGALAIAPSARNTLWA
                     GTGETFFIRPTTTLGNGVYRSTDAGRSWQHLGLDSTGRIARIVIHPYNPDIVLVCALG
                     RSYGPSPDRGVYRTADGGKTWTRVLFVDPNTGCSDLAIDPGDPNTLFAGMWQFEIKTW
                     RLHSGGPGSGLWVSRDGGVTWQRLAGHGLPAASHAIGKIAVAVAPSDPNTVYTLIEDT
                     DPSLYRSADRGNTWQLVSRNHDMAERAPYYVRFAVSPDDEDRLYFVTVRFSVSLDGGR
                     TLARSGFGAGGDNHDIWIDPENPDRYMLAHDGGASITLNRGRSYTRIVLPIAQMYHVA
                     TDTRVPYFVYGNRQDGGSYRMPSRSLSGALTEGQWGAVGGCEAGFAIPDTVDNATVWS
                     GCYDGGLEVYDVRTDHARNVRVWPEAGYGWRPADVKYRWNWNFPIAVSPHDHSRVYVG
                     SQFVHRTTDGGASWQVISPDLTRNDTTHQQSSGGVTTDNLMTFDGATLFAIAESPVRD
                     GVIWAGSNDGLVQVTQDGGTTWTNVTANIPALPPWGKVTNIEPSHFDAGTAYLAMDLH
                     ELDDFGSYIYKTTDYGKTWKRISTTFPRSVLSFVHMVAEDPVRRGMLFAGTENGLYVT
                     LEDGARWLPLQTNLPHAPVSWVTVQPRFHDLVVATYGRGFWILDDISPLEQSDAAALT
                     RRAHLFAPSAAYRFRATQGVAAAPNSAVRGENPGSGAALTYYLSSAVADTGATAASPP
                     ESPAPAGRDTASHRTTIKLVVLAANGDTVRVLVPARKPGLNRVWWDLRSTPPAVPRLR
                     TSPPDARWVRVGEDGTRPLVTWDLDLSMRGPFVPPGTYTVRLVVGDSAQSQPLVVLRD
                     PNTTATDSSLLAQSRLGRMIRDEQDSVARMINRLEWVRAQLGALSAQLRGDSTLARDS
                     SARRLAARADSLDRRAIAVEGSLFDVHLTGAREDAF"
BASE COUNT          623 a         1599 c         1464 g          651 t
ORIGIN      
        1 agcgatcgac agccggctcg acgccttcgg ccggagcttc atgctgcggc ccggcatgcc
       61 ggtcgtcgaa ccccgtctga cgatccgcaa cggaaccatc gcccggttag agatcgtgca
      121 gcatgccgcg cagccggcgc tctccggcgc tgcgccgtgg ccgatgcgga cgcaggtcct
      181 gctcgcctat cgcggcgcgc agccggtgcg cctccccatc gccctgcggg gaacggtgac
      241 ggaggttccg gaggcccgcg gccgttcggc accgtcgtgg atcttcccca acgcgggcga
      301 ctacggctac ttcctctcgc tgctcgacac ggccagtgtg agcgcgctcg agcgtggggg
      361 cctgcgcgag gtgcacgacg ggctgctccg cgcgatgctc tggggcgcgc tgtgggatca
      421 ggtgcgggag gcgcgtctcg acccggcccg gttcgcgcgg ctcgtcctgg ccgagctgcc
      481 attcgaacgc gacgaacaga tcgtccccgc gctgctcggc cgcttgggtc gcgcgctcga
      541 tgcgtacctc gcgcccgccc gggcgcggcc catcggcgcg gcggccgagc gcgtgctgtg
      601 ggagacggcg ggcgacacga cgctggccta cggcattcgt cgcgcggcgc tcgatgcgtt
      661 cctcggcctc gtcacgaccc cggaggggat cgcggacatg aagacgctgc tgcacgccga
      721 ctccgcggcc ggcgggccac tgcgcgatcc gacccggtgg agcatcgtga accggttgct
      781 catcctcggc gatgcggagg ccgagccggc gctcgtcgcg caggcgacgc gcgacacgac
      841 tccggacggg cgccgccgcg ccttcgtcgc gggtgccgcc cggcccagcg cggacgtgaa
      901 ggccgactat ttccggcgct ggttcgccga cgcgtcgctc aacgaggact gggccagcgg
      961 cagcttcggc acgttcaacg cgatcgagca tcaggcgctc accctgccgt tcctccggcc
     1021 ggcgctcgat tccctgccct acatccagca ccatcgccgg atcttcttcc tcggcgcctg
     1081 gctgggttcg ttcctgggtg gtcagtcgag ccgggcggcg ctggaggtgg tacgcgcgta
     1141 tctcgccgat catcccgacc tgccggccga tcttcgccag aaggtgctgc agagcgccga
     1201 cgagctggag cgcacggtgc ggatccgggc gctgtgggga gcgcagtagc tcctcgcgca
     1261 gccgcgccgc agaccgcatt cttacgcctc cactccccgt gaggccgtcc ccgatgtcgc
     1321 gtctcccatc gctcgccgtg ctcctggtgc tcgccgcccc gcctgcccgc gcccagcagg
     1381 tcgacacgac cctctggacg aacctgcgat tccggttcat cggacccgac gggaaccgcg
     1441 cctcggcgat cgtcggcgag ccgggcaacc cgttcgtcgc ctacgtcggc gcggcctcgg
     1501 gcggcgtctg gaagaccgaa gacggcggcg tgcactggcg agcggtgttc gacagcgagc
     1561 cggcgcaggc gatcggtgca ctcgccatcg cgccgtcggc ccgcaacacg ctctgggccg
     1621 ggacgggaga gaccttcttc atccgtccga cgaccacgct gggcaacggc gtgtaccgct
     1681 ccaccgacgc gggccggagc tggcagcacc tggggctcga ctccaccggc cgcatcgccc
     1741 gcatcgtcat ccacccctac aacccggaca tcgtgctggt gtgcgcgctg ggacgatcct
     1801 acggcccctc ccccgaccgt ggcgtgtacc ggaccgcgga cggcgggaag acatggacgc
     1861 gggtgctctt cgtggatccg aataccggat gctccgacct ggccatcgat cccggcgacc
     1921 cgaacaccct gttcgccggg atgtggcagt tcgagatcaa gacctggcga ctccacagcg
     1981 gaggaccggg cagcggcctc tgggtgtcgc gcgacggcgg cgtgacctgg cagcggctcg
     2041 cgggccacgg actgcccgcg gcgagccatg caatcggcaa gatcgcggtg gcagtggcgc
     2101 cctccgaccc gaataccgtg tacacgctga tcgaggacac cgacccgagc ctgtaccgca
     2161 gcgccgaccg cggcaatacg tggcagctcg tgagccgcaa tcacgacatg gccgagcggg
     2221 cgccctacta cgtgcgcttc gccgtctcgc ccgacgacga ggaccggctg tacttcgtca
     2281 cggtccgctt cagcgtctcc ctggacggcg ggcggaccct cgcgcgctcc ggcttcggtg
     2341 cgggcggcga caaccacgac atctggatcg atccggagaa tccggaccgt tacatgctcg
     2401 cccacgacgg cggcgccagt atcacgctga atcgcggccg cagttatacg cggatcgttc
     2461 tgccgatcgc gcagatgtac catgtcgcga ccgacacccg cgtcccctac ttcgtctacg
     2521 gcaaccgcca ggacggcggc tcgtaccgca tgccgagccg cagtctctcc ggcgcgctga
     2581 ccgaggggca atggggggcc gtcggtggtt gcgaggcggg gttcgcgatc cccgacacgg
     2641 tcgacaacgc gaccgtgtgg tcgggatgct acgacggcgg tctcgaggtc tacgacgtgc
     2701 gtaccgacca tgcgcgcaac gtccgcgtct ggccggaagc aggatatggc tggcgacccg
     2761 ccgacgtgaa gtaccgctgg aactggaatt tccccatcgc ggtctccccc cacgaccaca
     2821 gccgggtgta cgtcggcagc cagttcgtgc accgcaccac tgatgggggg gcgagctggc
     2881 aggtgatcag ccccgacctg acccgcaacg acaccacgca ccagcagagc tcgggcggcg
     2941 tcacgaccga caatctcatg accttcgacg gggcgacgct gttcgccatc gccgaatccc
     3001 ccgttcggga cggggtgatc tgggccggct ccaacgacgg cctggtccag gtcacgcagg
     3061 acggcggcac cacctggacg aacgtcaccg ccaacatccc ggccctgccg ccgtggggga
     3121 aggtgaccaa catcgagcca tcgcatttcg acgcgggcac ggcctacctg gccatggacc
     3181 tgcacgagct cgacgatttc ggttcgtaca tctacaagac gacggattac ggcaagacgt
     3241 ggaaacggat ttcgaccacg ttcccccgct ccgtcctctc cttcgtccac atggtggccg
     3301 aggatcccgt acgacgcggc atgctcttcg ccggcacgga aaacggactc tacgtcacgc
     3361 tcgaagacgg cgcgcgctgg ctgccgctgc agaccaacct gcctcacgcg ccggtgtcgt
     3421 gggtgacggt gcagcccagg tttcacgacc tcgtggtcgc gacgtacggg cgggggttct
     3481 ggatcctgga cgacatctcg ccgctcgagc agtcggacgc cgccgcgctc acccgtcgcg
     3541 cgcacctctt cgcaccgtcc gccgcgtacc ggttccgtgc cactcagggt gtcgcggccg
     3601 cccccaacag cgccgtgcgg ggcgaaaacc ccgggtccgg cgcggcgctg acgtattacc
     3661 tctcgtccgc cgtggctgat actggcgcca cggcggcttc accgcccgag tccccggcgc
     3721 ccgcgggtcg cgacacggcg tcccaccgga ccacgatcaa actggtcgtg ctggccgcca
     3781 acggcgacac ggttcgcgtc ctcgttcccg cacgcaagcc cgggttgaac cgcgtctggt
     3841 gggacctccg ctccacccca cccgcggtcc ccagactgcg cacgtcgccg cccgacgcgc
     3901 gctgggtgcg cgtcggggag gatggcacgc gcccgctcgt gacctgggac ctcgatctct
     3961 ccatgcgcgg accgttcgtg ccgcccggga cctacaccgt gcgcctcgtc gtgggcgaca
     4021 gtgcccagag ccagccgctc gtggtgctgc gcgaccccaa cacgacggcc accgactcgt
     4081 cgctgctggc acagagccgg ctgggccgga tgatccgcga cgagcaggac agcgtcgcgc
     4141 gcatgatcaa ccggctcgag tgggtgcgcg cccagctcgg ggcgctctcg gcccagctcc
     4201 gtggggattc gacgctggcc cgcgactcga gcgcgcggcg gctcgcggcc cgtgccgatt
     4261 cgctcgatcg ccgcgcgatt gccgtcgagg gcagcctgtt cgacgtccac ctgaccgggg
     4321 cccgggagga tgcgttc
//
LOCUS       DBNGZW010000620         5894 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1150416, whole genome shotgun sequence.
ACCESSION   DBNGZW010000620 DBNGZW010000000
VERSION     DBNGZW010000620.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5894)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5894)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5894
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1150416"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..315)
                     /locus_tag="AC16JC_14555"
     CDS             complement(52..315)
                     /locus_tag="AC16JC_14555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165968.1"
                     /translation="MTASRIAEIQRQLTEGLARIDPHHRLVGRPTSFRVIDGRTLEIT
                     FRDVAGIAEAEVLGVKRMLGTECYCTVSPQTAEQLTVRFVVPL"
     gene            complement(312..1262)
                     /gene="queG"
                     /locus_tag="AC16JC_14560"
     CDS             complement(312..1262)
                     /gene="queG"
                     /locus_tag="AC16JC_14560"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA];
                     GO_process: GO:0008616 - tRNA queuosine(34) biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HIR5165969.1"
                     /translation="MDSGAAELTRRARSLGFDAVGVAPLEPNPHARELDHWLEAGYAG
                     TMSYLHRQAAKRRDPRVIMPEARVAVVTLTNYFHSQGIVPDSDRAPGEHASPVRVAQY
                     AWSRDYHAVLSDRLERLAAAIRDLYPGARTRCYVDAGPVPERELAQRAGLGWIGKNTM
                     LINPEIGSFTFLGSVLTDAPLQPDAPFAADRCGTCRACLDACPTGAFAAPHLLDARRC
                     ISYVTIEHRGAFTDEQREWIGEWLFGCDVCQDVCPWNVRFARPTLDAELAPRALPDPV
                     ELAVIDAATFDLRYADTAFSRPGHAGMRRNAAAVRPAGAP"
     gene            complement(1275..1541)
                     /locus_tag="AC16JC_14565"
     CDS             complement(1275..1541)
                     /locus_tag="AC16JC_14565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165970.1"
                     /translation="METPASWTALIARLALRAVIQPRLAVDLLRLTWSFRARGWYRRP
                     PFLPLPPPEYMRWRMFTAYGDEAAVPPVEDVVRFARWRRETMRL"
     gene            1564..2754
                     /locus_tag="AC16JC_14570"
     CDS             1564..2754
                     /locus_tag="AC16JC_14570"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR5165971.1"
                     /translation="MTLASGVAGGPVGPAADALPLSRFRSEFPIFRDRIYLNTCSLGP
                     LGERARRRLAGFLDSWQARGAAAWYDDWWAALAELRARYGRVIGAEGRDIALAPSVSA
                     AVGAVASAIDYRKRPRVVVTSLDFPTVVYQWLAKRPHGVEVVLVESPDQVSVPVEAIA
                     RAVDDRTALVATSHVYFTSGAIQDIAAVADVAHRRGALCLVDAYQGVGQVPVDAPATG
                     VDFLIAGGLKWLLGGTGIVFCYVREGLARELAPSAAGWFGHRDQFAFEPRALEFHDDA
                     RRFEQGTPALPSVFTQLGGLEYIEEIGVAAIRRATAALAEDLVARAREAGFRPKVAAT
                     PDTRSAIVMLPAADPAAAVRDLAASGIVADARPGHVRLSPFFYNLQEDHAAALERLAA
                     HRSH"
     gene            2711..3454
                     /locus_tag="AC16JC_14575"
     CDS             2711..3454
                     /locus_tag="AC16JC_14575"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR5165972.1"
                     /translation="MPQRSSDLPRTGVTEDERLLTTVPSTERRAFVHTDPWRVLRIMG
                     EFVEGFDTLSDVRLAVTFFGSARTKPGDPYYERAVETARLLAKQGFPIITGGGPGIME
                     AANRGAQEGNGLSIGCNIELPFEQGTNAFVERSINFRYFFVRKTMFVKYSTAFIVFPG
                     GFGTMDELFEALTLIQTGKVKHFPVVLFGREYWGGLAEWLAERVAGEGKIDAKDLELF
                     HVTDHPAEAVGLVVKAREERAQARSSRKS"
     gene            3460..4596
                     /locus_tag="AC16JC_14580"
     CDS             3460..4596
                     /locus_tag="AC16JC_14580"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007417957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homospermidine biosynthesis protein"
                     /protein_id="HIR5165973.1"
                     /translation="MTSSGFLRGARIDPQAITGRETVAELVERTFLAYNAGRLAEACR
                     LYTERMLAPDATVGMSLTRAMTPAGLGMSTIIPLIEAGFIDWIVSTGANLYHDAHFGL
                     GLSMHRGNPFADDVELREAGVVRIYDIFCHYDVLLSTDAYIREVSARPEFQRPMSTAE
                     YHYLLGGYIAERERALGLSRRSVLAVAHGCGVPIYTSSPGDSSIGMNVAEQALAGSQL
                     RFDVSADVNETAAIVFGAKSSGGKSGVLLIGGGSPKNFVLQTEPQIQEVLGISEKGHD
                     HYLQMTDARPDTGGLSGATPAEAVSWGKVDPEQLPGTVVCYLDNSIGFPILAAYALAR
                     HPPRPLKRLYERREALMADLRAAYAAARTERDARAQAAGSGRER"
     gene            4598..>5894
                     /locus_tag="AC16JC_14585"
     CDS             4598..>5894
                     /locus_tag="AC16JC_14585"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5165974.1"
                     /translation="MTRPASDSRTYPFYVLGLLTLINLFNYADRNVVFAVFEPLKRDL
                     QLSDQQLGWIGSAYIIVLSLAALPLGVLGDLRTRRGVIAWGVGLWSLFTSLGGAVRNF
                     WQLFLCRAMVGVGEAGYGPVSQALIAEYFKEKRRALAIGVYSVGMALGGVLGIWLGGL
                     LAEAYGWRLAFLWLGLPGFGLAILASRLREPKRRPPTAIAVTLRRFTTTGMRQAARYG
                     APLIGLSALGAVVSGVLALVRVIPTEMVVAVFGMFVGVGVAWTVARLVPVALRRTSEA
                     QEVAATAFEEFVEAAAVVLRVPTLIWVFVGGALVTFAVNGLIAWAASFMQRVHGFSVS
                     DVGREFGLWGLAGGALGALTGGRLADLLFERWSGARVAVSGAGFLLGSPVCVALILTN
                     DLRLFAPLVFTTYFFYTWYNGPLAAVIFDVVPAAIRASVM"
BASE COUNT          829 a         1968 c         2126 g          971 t
ORIGIN      
        1 gatccgctgg ggcaatccga ccgagcttcc cgtgctgcgc ctggtaccgg cctagagtgg
       61 tacgacgaac cgcacggtga gttgctcggc cgtttgcggc gagacggtgc aatagcattc
      121 ggtgccgagc atccgtttga ccccgagcac ttccgcttcg gcgatccccg cgacatcgcg
      181 gaacgtgatc tcgagcgtcc gtccatcgat cacccggaaa ctcgtgggcc ggccgacgag
      241 tcggtggtgc ggatcgatgc gggccaggcc ctcggtgagc tggcgctgga tctcggcgat
      301 gcggctcgcc gtcatggggc gccggcggga cggacggccg cggcgttgcg gcgcatcccg
      361 gcgtggcccg gccgggagaa ggccgtatcg gcgtagcgga ggtcgaaggt ggctgcgtcg
      421 atcaccgcca attccaccgg atcggggagg gcgcgcggcg cgagctcggc gtccagggtc
      481 gggcgcgcga aacgcacgtt ccaggggcac acgtcctgac atacgtcgca tccgaacagc
      541 cactcaccga tccactcccg ctgctcgtcg gtgaacgccc cgcgatgctc aatggtcacg
      601 tacgagatgc aacggcgcgc gtcgagcaga tggggggcgg cgaacgcgcc ggtcgggcag
      661 gcgtcgaggc aagcgcggca cgtgccgcag cggtcggcgg cgaacggtgc atcgggctgc
      721 agcggggcgt cggtcaagac tgagccgaga aacgtgaacg atccgatttc cgggttgatc
      781 agcatcgtgt tcttgccgat ccagccgagt cccgcccgct gcgcgagctc ccgctcgggc
      841 accggtccgg catcgacgta acagcgggta cgcgctccgg ggtacaggtc gcgaatcgcg
      901 gcggcgaggc gctcgaggcg gtcgctcagc accgcgtggt agtcgcgaga ccaggcgtac
      961 tgggcgaccc ggaccggcga tgcgtgttcg ccgggagcgc ggtcggagtc cggtacaatt
     1021 ccctgggaat ggaagtagtt ggtcaacgtc accaccgcga cccgggcctc cggcatgatc
     1081 acgcgcgggt ctctgcgctt cgccgcctga cggtgcaggt agctcatcgt gccggcgtat
     1141 ccggcctcga gccagtggtc gagctcccgc gcgtgcgggt tcggctcgag cggcgcgaca
     1201 ccgaccgcgt cgaagcccag cgaccgcgcc cggcgcgtga gctcagctgc gccgctatcc
     1261 atacgtcctg gtcatcagag ccgcatcgtc tcccgccgcc agcgcgcaaa ccggacgacg
     1321 tcctcgaccg gcgggaccgc ggcttcgtcg ccgtaggccg tgaacatgcg ccagcgcatg
     1381 tactccggag gtggcagcgg gagaaacggc gggcggcgat accacccccg agcccggaag
     1441 ctccacgtca ggcgcaacag atcgaccgca agccggggct gtatgacggc gcgcaacgcc
     1501 agacgggcga tcagcgcggt ccaactcgcc ggagtctcca tagcttctgt atattcagcg
     1561 tctatgacgc ttgcaagcgg cgtcgcgggc ggtccggtgg gcccggcggc cgacgcgctc
     1621 cccctgagcc gcttccggag cgaattcccg atcttccggg atcggatcta tctgaacacc
     1681 tgctccctcg ggcccctggg agagcgggcg cgccgcaggc tcgcggggtt cctggactcc
     1741 tggcaggcac gcggggccgc cgcgtggtac gacgactggt gggcggcgct ggcggagctg
     1801 cgcgcccgct acggccgggt gatcggcgcc gaggggcgcg acatcgcgct cgcccccagc
     1861 gtctccgccg cggtgggcgc ggtggcgagt gccatcgatt accggaagcg cccccgcgtg
     1921 gtcgtcacct cgctcgattt tccgacggtc gtctaccaat ggctcgccaa gcggccgcat
     1981 ggggtcgagg tcgtgctggt cgaaagcccc gatcaggtga gcgtcccggt cgaggcgatc
     2041 gcgcgcgcgg tggacgaccg cactgcgctg gtcgccacgt cgcatgtgta cttcaccagt
     2101 ggcgcgatcc aggatatcgc ggcggtcgcc gacgtggcgc accgacgtgg ggccctgtgc
     2161 ctcgtcgacg cgtaccaagg ggtcggccag gtgcctgtgg atgcgccggc gacgggcgtg
     2221 gacttcctca tcgccggcgg cctcaagtgg ctgctcggcg gtacggggat cgtcttctgt
     2281 tacgtgcgcg agggactggc ccgcgagctc gcgccttcgg cggcgggatg gttcgggcac
     2341 cgcgaccagt tcgcgttcga gccgcgtgcg ctcgagttcc acgacgacgc ccggcgcttc
     2401 gagcagggga cgcccgccct cccgtcggtg ttcacccagc tcggcggact ggagtacatc
     2461 gaggagatcg gcgtcgcggc gatccggcgg gccaccgcgg cgctcgccga ggatctcgtg
     2521 gcgcgcgccc gggaggcggg attccggccc aaggtggccg ccacgccgga cacgcgctcg
     2581 gcgatcgtga tgcttcccgc cgccgatccc gccgcggcgg tgcgcgacct cgcggcgagc
     2641 ggcatcgtcg ccgacgcccg ccccggccac gttcgcctgt ccccattctt ctacaacctc
     2701 caggaagacc atgccgcagc gctcgagcga cttgccgcgc accggagtca ctgaagacga
     2761 acgcctgctc accaccgtcc cgtcgacgga acggcgggca ttcgtgcata ccgatccgtg
     2821 gcgcgtgctg cgcatcatgg gggagttcgt cgaggggttc gacaccctgt ccgacgtgcg
     2881 cttggcggtc acattcttcg ggtcggcccg gaccaagccg ggcgatccct actacgagag
     2941 ggcggtcgag acggcccggc tgctcgccaa gcaggggttc cccatcatca cgggcggcgg
     3001 ccccggaatc atggaggccg ccaatcgcgg cgcgcaggag gggaacggac tttcgatcgg
     3061 ctgcaacatc gaattgccgt tcgagcaggg gaccaatgcg ttcgtggagc ggtcgatcaa
     3121 tttccgctat ttcttcgtcc gcaagacgat gttcgtgaag tattcgacgg cattcatcgt
     3181 tttcccgggt gggttcggca ccatggacga gctgttcgaa gcgctcacgc tgatccagac
     3241 gggaaaggtg aaacacttcc cggtcgtgct cttcggacgg gagtactggg gtggattggc
     3301 ggagtggctg gcggaacggg tcgccgggga gggcaagatc gacgccaaag atctggagct
     3361 gttccacgtg acggatcatc ccgcggaagc cgtcgggctc gtggtcaagg cgcgggaaga
     3421 acgggcgcag gcgcgctcct cgcggaaatc ctgatcccga tgacatcctc cggttttctg
     3481 cgcggcgcgc gcatcgaccc tcaggcgatc accggccgtg agaccgtcgc cgagctggtg
     3541 gagcgtacct tcctggcgta caacgcgggg cggctggcgg aagcgtgccg cctctacacg
     3601 gagcggatgc tcgcgccgga cgccacggtc gggatgagtc tcacccgggc catgaccccc
     3661 gcggggctgg ggatgagcac catcatcccg ctcatcgaag ccgggttcat cgactggatc
     3721 gtctccaccg gcgcgaacct ctatcacgac gcgcatttcg ggctgggcct gtcgatgcat
     3781 cgcggcaacc cgttcgccga cgacgtggag ctgcgcgagg cgggcgtggt gcggatctac
     3841 gacatcttct gccactacga cgtcctactc tccaccgacg cctacatccg ggaagtctcg
     3901 gcgcgccccg aattccagcg ccccatgagc accgccgagt atcactacct gctgggcggc
     3961 tacatcgccg agcgcgagcg ggcgctggga ctgtcccgcc gctcggtgct cgccgtggcg
     4021 cacgggtgcg gcgtgccgat ctacacctcg tcgcccggcg actcctcgat cgggatgaac
     4081 gtcgccgagc aggcgctcgc gggctcgcag ctccgtttcg atgtcagtgc ggacgtcaac
     4141 gagaccgcgg ccatcgtgtt cggggccaag tcgagcggcg gcaagagcgg cgtcctgctg
     4201 atcggcgggg gcagccccaa gaacttcgtg ctgcagacgg agccgcagat tcaggaggtt
     4261 ctggggatca gcgagaaggg gcacgaccac tatctccaga tgacggatgc ccggcccgac
     4321 accggcgggc tctcgggcgc cacgccggcg gaggcggtca gctgggggaa agtcgatccg
     4381 gagcagctgc cggggacggt ggtctgctat ctcgacaact cgatcggctt cccgatcctg
     4441 gccgcgtacg ccctggcgcg acatccgccc cggccgctca agcggctgta tgaacggcgt
     4501 gaagcgctga tggcggacct gcgggcggcg tacgcggcgg ccagaacgga gcgcgacgcg
     4561 cgggcgcagg cggcgggcag cgggcgcgag cgctgacgtg acccgaccgg ccagcgactc
     4621 ccggacgtat ccgttctacg tcctggggct gctcacgctg atcaaccttt tcaactatgc
     4681 ggaccgcaac gtcgtcttcg cggtcttcga acccctcaag cgcgatctgc agctctccga
     4741 ccagcagctg ggctggatcg gttcggccta catcatcgtt ctgtcgctgg cggcgctgcc
     4801 cctcggcgtg ctcggcgacc tgcgtacccg tcgcggcgtg atcgcctggg gggtggggct
     4861 gtggagtctg ttcacgtccc tcggcggcgc ggtcaggaat ttctggcagc tgttcctgtg
     4921 ccgggcgatg gtcggcgtgg gcgaggccgg ctacgggccc gtctcccagg cgctcatcgc
     4981 cgagtacttc aaggaaaagc gccgcgccct cgccatcggc gtgtactccg tgggaatggc
     5041 gctgggcggc gtgctcggga tctggctcgg cgggctgctc gccgaagcgt atggatggcg
     5101 gctggctttc ctctggctcg ggcttcccgg cttcggcctg gcgatcctcg cttcccggct
     5161 gcgggagccg aagcgccgtc cgcccacggc catcgccgtc acgctgcgcc ggttcacgac
     5221 cacgggaatg cggcaggctg cgcgctacgg cgcgccgctg atcggactgt cggcgctggg
     5281 agcggtagtg agcggcgtgc tggcgctggt ccgggtgatc ccgaccgaga tggtcgtggc
     5341 cgtcttcggc atgttcgtgg gcgtgggcgt ggcctggacc gtggcgcgcc tcgttccggt
     5401 cgcgttgcgc cgcacgtcgg aagcccagga agtggcggcg accgcgttcg aggagttcgt
     5461 ggaagccgcg gccgtggtgc tgcgcgtgcc gacgctcatc tgggtgttcg tgggcggggc
     5521 actcgtgacg ttcgccgtga acgggctcat tgcctgggcc gcgagcttca tgcagcgcgt
     5581 ccacggcttt tcggtgagcg acgtgggacg tgaattcggc ctctggggcc tggccggcgg
     5641 cgcgctgggc gccctgacgg ggggccgtct cgcggacctg ctgttcgagc gctggagcgg
     5701 cgcgcgcgtg gcggtctccg gcgccggctt tttgctcggc tcacccgtgt gcgtggcgct
     5761 gatcctgacg aacgatctcc ggctgttcgc gccgctcgtg ttcacgacct atttcttcta
     5821 tacgtggtac aacggcccgc tcgctgcggt gatcttcgac gtcgtgccgg ccgccattcg
     5881 ggcgtcggtg atgg
//
LOCUS       DBNGZW010000621         6300 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_262550, whole genome shotgun sequence.
ACCESSION   DBNGZW010000621 DBNGZW010000000
VERSION     DBNGZW010000621.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6300)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6300)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6300
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_262550"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..396)
                     /locus_tag="AC16JC_14590"
     CDS             complement(<1..396)
                     /locus_tag="AC16JC_14590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165975.1"
                     /translation="MGAKPTRGAIARYHLVRWVWVAGLAALALFAFPSGVTNLARLLT
                     IGVRADRQVVAPFDFVVTKTDEELRREADAVAASVRPIYDFRAEAYDSAQSWARGFFA
                     SLDSVAKRGREAVLLAAQRAGVPLAGAEAD"
     gene            complement(396..1358)
                     /locus_tag="AC16JC_14595"
     CDS             complement(396..1358)
                     /locus_tag="AC16JC_14595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009381878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR5165976.1"
                     /translation="MSDDAVRRLSVEGIDLLSLAGVNEGNLVELTRRLGVRVSLRGDA
                     LTVQGPAAAVERAAPVAQAMVDLARMGETLDVHDVERLVLEGEAGTLVDAGTGTGETK
                     IILPGLRRVIQPKTAGQREYLHLIAQHDIVVGIGPAGTGKTYLAVAAAVDALARKRVR
                     RIVLARPAVEAGESLGFLPGDLQEKVDPYLRPLYDALEDMMPRDRVQKALETRAIEIA
                     PLAYMRGRTLSDAFVILDEAQNATGMQMKMFLTRLGVNSRAVITGDKTQIDLANREES
                     GLLQIERILPGIEGIGFCYLTDTDVVRHRLVRDIIRAYAEDAQG"
     gene            complement(1355..3124)
                     /gene="aspS"
                     /locus_tag="AC16JC_14600"
     CDS             complement(1355..3124)
                     /gene="aspS"
                     /locus_tag="AC16JC_14600"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011232049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HIR5165977.1"
                     /translation="MWRTHRCGALRRTEVGQTVRLGGWVHRRRDLGGIVFLDLRDRDG
                     LVQVAIGPAWAAAEAVASAQAVGVESVVVVEGEVVARPANMQNVELATGEIEVHARSL
                     QVVGPAETPAIPVARGKGEELPAEELRLKYRHLDLRRPERQANLMLRHRLLQRARRTL
                     SDLGFLEIETPILTKPTPEGARDYLVPSRVHAGEFYALPQSPQIYKQLLMVSGFDRYF
                     QLARCFRYEDLRNDRQPEFTQIDIEASFVGQEDVIGFVEAVLTALWEECGHTIPRPFP
                     RLKWTDAMERYGSDKPDLRYELEIADWTAAVRPLAVPFFTSALSATGGKNGGRVRGLA
                     VRGGGALSRKDVDQLAAAAKQLGGAGLAWVKRQGEELSGSVAKQFTVEALGRLGVGDG
                     DAALMTVGPDHVTSPVLDRVRQDVLRRLGVAPRTAHAFCWVVDFPLFEPDPATGAPVF
                     AHHPFTSPHPDDVAKLDHEPFACRALHYDAVYNGNELGSGSIRITDPAVQRRVFDHLG
                     LTRADAEARFGFLLGALAAGAPPHGGFAIGFDRTTMLLAGAGSLRDVIAFPKTTAARA
                     LFEGAPSPARAEDLATLHIRTVP"
     gene            complement(3139..3594)
                     /locus_tag="AC16JC_14605"
     CDS             complement(3139..3594)
                     /locus_tag="AC16JC_14605"
                     /inference="COORDINATES: protein motif:HMM:NF012579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR5165978.1"
                     /translation="MRLTDLLTPDRIRIPLRASDKEGVLRELVGLLLPGDGALSEEVL
                     AAILERERQFPTGIGHGVAVPHGRTPAMGALAIVAGTSPNPVRYETIDGQPVRVFFMV
                     AGPEALAGAHVKALSRISRLVCQEGVRARLLAARTPQEFYHAILEAEGA"
     gene            3703..4575
                     /locus_tag="AC16JC_14610"
     CDS             3703..4575
                     /locus_tag="AC16JC_14610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165979.1"
                     /translation="MACVVLVPRLASQAGAGSELWRLAAATIPVPAALATGVVASYWN
                     PAQDPAGPGQIGLDLIQTPVAVGATGLIGGLRASLHHVGSLGFVYGRMGLGNLVRTID
                     SPDPAGTTIPFYTQTAGLTWARALTATTVGAAITYHDTRLDDTDTQHWTLDLGLSRSL
                     WQDRIRIAAGTRGLRRLGSDAAQDVNVGVQVRAWRGTLWGTTGGTLVARYGFAFGHPG
                     GADHQIGAGLDVGTSVSCDAIVARETSYGNAAWRSAAGIRIAVGRYRLSLARDGGVSD
                     LGSTFRVGLEARRK"
     gene            4572..5642
                     /gene="rlmN"
                     /locus_tag="AC16JC_14615"
     CDS             4572..5642
                     /gene="rlmN"
                     /locus_tag="AC16JC_14615"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR5165980.1"
                     /translation="MTDLLSLTPARAAETLAEWLQARGESAYRLRQIVPRLWQRPVGS
                     WRDASDLPAPLRTALEDAFPLPRLALAAHQVSQDGTEKFLWRLPDGEAIESVLIPEGR
                     RRTLCISSQVGCALGCVFCATGRMGWRRDLTAGEIAGQWREVVLLGEDRRPTNVVFMG
                     MGEPLLNWEAVDTALTILNQPDGCGIGARHITLSTVGILPNLARFAARPEQFRLALSL
                     HAATHPARLALMPIEKKYPLAEVLQALRQFRRRVTLEYVLIGGKNDSLEAADALAKIA
                     GPLAAHVNLLPLHPGGAPDLTPSPHPQMLAFERRLRERGIEAVLRRSRGLDISAACGQ
                     LRVEVEGRPRGKVRAQQHAHVD"
     gene            complement(5574..6011)
                     /locus_tag="AC16JC_14620"
     CDS             complement(5574..6011)
                     /locus_tag="AC16JC_14620"
                     /inference="COORDINATES: protein motif:HMM:NF024791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HIR5165981.1"
                     /translation="MGPETGDGGLLERARLGLIVGLLLCAGCGHDEARPAFAVPGQDG
                     PRIVVEVLNVTRRPGLARTGTLVLRQAGIDVVGYGNAVPLPARSDSTRILVRRGDRAA
                     GERVRRALQVGTVVLQPDSTRLVDVSVLLGADFAARASLDFHP"
     gene            complement(5957..>6300)
                     /locus_tag="AC16JC_14625"
     CDS             complement(5957..>6300)
                     /locus_tag="AC16JC_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165982.1"
                     /translation="DAPAATELDHGPLCADRLARDGERDQGVLDAVRYHSLGFAGWDD
                     VGRMLYVADYLEPGRRFDDARSTALRERMPEECGAVLHEVARHRIEWVLRSSWDLKPE
                     TVAFWNALVSA"
BASE COUNT          924 a         2353 c         2123 g          900 t
ORIGIN      
        1 atccgcctcg gcacccgcca gcggcacgcc ggcccgttgc gcggcgagca gcacggcctc
       61 acggccccgt ttcgccacgc tgtccagcga ggcaaagaac ccccgtgccc acgactgcgc
      121 gctgtcgtag gcctccgcgc gaaaatcgta gatcggccgg acggaggccg ccacggcgtc
      181 cgcctcccgg cgcagctcct cgtccgtctt ggtcaccacg aaatcgaacg gcgccaccac
      241 ctgccggtcg gcgcggacgc cgatggtgag caggcgggcc agattcgtga cgcccgacgg
      301 aaacgcgaac agcgccaggg cggcgagtcc cgcgacccac acccagcgca cgaggtgata
      361 gcgcgcaatg gccccccgcg tcggcttggc gcccatcagc cctgcgcatc ctcggcgtag
      421 gcgcggatga tgtcccgcac cagccgatgc cggaccacgt ccgtgtcggt gaggtagcag
      481 aagccgatgc cctcgatacc cggcaggatc cgctcgatct gcagcagccc cgactcctcg
      541 cggttcgcga gatcgatctg cgtcttgtcg ccggtgatca cggcgcgcga gttcacgccc
      601 aggcgcgtga ggaacatctt catctgcatg ccggtcgcgt tctgcgcctc gtcgaggatg
      661 acgaaggcat ccgacagcgt gcggccgcgc atgtaggcga gcggcgcgat ctcgatggcg
      721 cgcgtctcga gcgccttctg cacccggtcg cgcggcatca tgtcctccag cgcgtcgtac
      781 agcggccgga ggtacggatc cactttctcc tgcaggtcgc cgggcaggaa gccgaggctc
      841 tcacccgctt ccaccgccgg ccgcgccagc acgatgcgcc gcacccgctt gcgggccagg
      901 gcgtcgacgg ccgccgccac cgcgagatac gtcttgcccg tgccggccgg cccgatcccc
      961 accacgatgt cgtgctgcgc gatgagatgg aggtactccc gctggcccgc cgtcttgggc
     1021 tggatcaccc ggcgcagccc ggggaggatg atcttcgttt cgcccgttcc ggtgcccgcg
     1081 tcgaccagcg tccccgcctc cccctcgagc accagccgct ccacgtcgtg cacgtcgagc
     1141 gtctcgccca tacgggcgag gtccaccatc gcctgcgcca ccggagccgc gcgctccact
     1201 gccgcggcgg gaccctgcac cgtgagcgcg tccccccgca gcgacacccg cacgcccagc
     1261 cggcgcgtga gctcgacgag attcccttcg ttcacgccgg cgagcgacaa caggtcgatc
     1321 ccctccaccg agagacgccg taccgcgtcg tcgctcacgg gaccgtccgg atgtgcaggg
     1381 tcgcgagatc ctcggcgcgg gccggcgagg gggccccctc gaacagcgcg cgtgccgcgg
     1441 tggtcttggg gaaggcgatc acgtcccgca ggctgcctgc cccggccagc agcatggtcg
     1501 tccggtcgaa cccgatggcg aaaccgccgt gcggcggcgc tcccgccgcc agggcgccga
     1561 gcaggaagcc gaagcgcgcc tcggcgtccg cgcgggtgag ccccaggtga tcgaacaccc
     1621 ggcgctgcac cgcgggatcg gtgatgcgga tggagcccga gccgagctcg ttgccgttgt
     1681 agaccgcgtc gtagtgcagc gcgcgacatg cgaacggctc gtggtcgagt ttcgccacgt
     1741 cgtcggggtg cggcgacgtg aagggatggt gcgcgaacac cggcgcgccg gtcgccggat
     1801 cgggctcgaa caacgggaag tccaccaccc agcagaatgc gtgcgccgtg cgcggagcga
     1861 cgccgaggcg ccgcaatacg tcctgccgca cgcggtcgag caccggcgac gtcacgtgat
     1921 cgggaccgac cgtcatcagc gccgcatcgc cgtcgccgac gccgagccga cccagggcct
     1981 cgacggtgaa ctgcttcgcg accgaccccg acagctcctc gccctgccgc ttgacccagg
     2041 cgagccccgc cccgcccagc tgcttggccg ccgcggcgag ctggtccacg tccttgcgcg
     2101 acagggcgcc gccgccccgc accgcgaggc cccgcacgcg tcccccgttc ttccctcccg
     2161 tcgcgctcag cgcggacgtg aagaacggca ccgccagcgg ccgcaccgcc gccgtccagt
     2221 cggcgatctc cagctcgtag cgcagatcgg gcttgtccga tccgtagcgc tccatcgcgt
     2281 ccgtccattt gaggcgcggg aacggccgcg ggatggtgtg tccgcattcc tcccacagcg
     2341 ccgtcagcac cgcttccacg aacccgatca cgtcctcctg cccgacgaag gacgcctcga
     2401 tgtcgatctg cgtgaactcc ggttgccggt cgttgcgcag atcctcgtag cgaaagcagc
     2461 gcgccagctg gaagtagcgg tcgaaccccg acaccatgag gagctgcttg tagatctgcg
     2521 gcgactgcgg cagcgcgtag aactccccgg cgtgcacccg cgacggcacg agataatcgc
     2581 gggcgccctc gggggtcggt ttggtgagaa tcggggtctc gatctcgaga aacccgagat
     2641 cggacagggt gcgccgcgcc cgctgcagca gacgatggcg cagcatgagg ttcgcctgcc
     2701 gctcgggccg gcgcaggtcg agatggcggt acttgagccg cagctcctcc gccgggagct
     2761 cctcgccctt gccgcgcgcg accgggatgg cgggcgtctc ggccggtccg acgacctgca
     2821 gcgaccgcgc gtgcacctcg atctcaccgg tcgcgagctc gacgttctgc atgttcgccg
     2881 gacgcgccac gacctcgcct tcgaccacga ccaccgactc gacgccgacg gcctgcgccg
     2941 aggcgaccgc ttcggcggcc gcccacgcgg gcccgatggc aacctgcacc aggccgtcgc
     3001 ggtcccgcag atcgagaaac acgatcccgc ccaggtcccg gcgccggtgt acccacccgc
     3061 ccaaccgcac cgtctgcccg acctccgtcc ggcgcagcgc gccgcaccga tgcgttcgcc
     3121 acatcgtccc gctcacccct atgctccctc cgcttcgaga atggcgtggt agaactcctg
     3181 cggcgtccgg gcggccagca gccgcgcccg aaccccctcc tggcacacca ggcgggagat
     3241 ccggctcaat gccttcacgt gcgctcccgc caacgcctcc ggacccgcaa ccatgaagaa
     3301 cacgcggacc ggctggccgt cgattgtctc atagcgcacg ggattcggac tcgttcccgc
     3361 cacgatcgcc agcgcgccca tcgccggcgt acgtccatgc ggcaccgcca caccatgacc
     3421 gatcccggtc gggaactgcc gctcccgctc caggatcgcc gccaggactt cctcactcag
     3481 tgccccgtct cccggaagca gtaaccccac cagctcccgc aggaccccct ccttgtcgct
     3541 cgcccggagg gggatccgga tccggtcggg agtcagcaga tcggtcaggc gcacggcgtt
     3601 ccgtaagtgg tgtaaactgt tgaatgtagt taagttggcc gaacaacgct acgccggata
     3661 gaacacctct caatgccgcc attctgccgc ttgttgccgt cattggcctg cgtcgtcctc
     3721 gtcccgaggc tcgcgagcca ggcaggtgcg gggagcgagc tgtggcgtct ggcggcggcc
     3781 acgatccccg tccccgccgc cctcgcgacg ggtgtggtcg cctcctactg gaatcccgcc
     3841 caggacccgg cgggtcccgg ccagatcggc ctggacctga ttcagacccc ggtcgcggtc
     3901 ggtgccaccg gtctgatcgg cggcctccgc gcgtcgctgc accacgtggg ctcgctggga
     3961 ttcgtctacg gccggatggg actggggaac ctcgtgcgga cgatcgacag tcccgacccc
     4021 gcggggacga cgattccctt ctacacgcag accgcgggac tcacctgggc gcgggcgttg
     4081 accgccacca cggtcggagc ggcgatcacc taccacgata cacgcctcga cgacaccgac
     4141 acgcaacatt ggaccctgga tctgggtctc tcccggtcgc tgtggcagga cagaatccgc
     4201 atcgcggcgg ggacgagagg gttgcgacgt ctggggagtg acgccgcgca ggacgtgaac
     4261 gtcggtgtac aggttcgcgc ctggcgcggc acgctctggg gcaccaccgg cggcaccctg
     4321 gtggcgcggt acggattcgc cttcggccat cccggcgggg ccgatcacca gatcggcgcc
     4381 ggcctggatg tgggaacatc ggtctcctgt gacgcgatcg tcgcccgtga gacgagctac
     4441 ggtaacgccg cgtggcgtag tgcggccggg atccgcatcg cggtcggccg gtatcgcctg
     4501 tcgctggcac gggacggggg cgtctccgac ctgggctcga ccttccgagt cggactcgag
     4561 gcgcggcgca agtgacggac ctgctctcgc tcactcccgc tcgggccgcg gagacgctcg
     4621 cggaatggtt gcaggcgcgt ggtgagtccg cgtaccgcct gcgccagatc gtgccgcggt
     4681 tatggcagcg tccggtcggc tcctggcgcg acgccagcga cctgccggcg ccgctgcgca
     4741 ccgccctgga agacgcgttc cccctgccgc ggctggcgct ggccgcccac caggtgtccc
     4801 aggacggcac cgagaagttt ctgtggcggc tgcccgatgg cgaggcgatc gaatcggtgc
     4861 tcatccctga ggggcgacgc cgcacgctgt gcatctcgtc gcaggtcggc tgcgcgctgg
     4921 gctgcgtgtt ttgcgccacg ggccgcatgg gatggcgccg ggatctcacc gcgggcgaga
     4981 tcgccggtca atggcgggag gtggtgctgc tgggcgagga ccggcgaccc accaacgtcg
     5041 tcttcatggg gatgggcgaa ccgctgctca attgggaggc ggtggatacg gcactcacca
     5101 tcctgaacca acctgacggt tgcgggatcg gggcccgaca catcacgctc tccacggtcg
     5161 gcattctgcc caacctcgcc cgtttcgcgg cgcgaccgga gcagttccgg ctggcgctgt
     5221 cgctgcatgc ggcgacgcat ccggcgcggc tggcgttgat gccgatcgag aagaagtatc
     5281 cgctcgccga ggtgctccag gcgctgcggc agttccggcg gcgggtgacc ctcgaatacg
     5341 tgctgatcgg cgggaagaac gactcgctgg aggccgcgga tgccctcgcg aagatcgccg
     5401 gcccgctcgc cgcgcacgtg aacctgctgc cgctgcatcc cggcggagcg cccgatctca
     5461 cgcccagccc gcacccgcag atgctggcgt tcgagcgacg gctgcgcgag cgcgggatcg
     5521 aagccgtgtt gcgccgcagt cgcgggctcg acatcagcgc cgcgtgcggg cagctacggg
     5581 tggaagtcga gggacgcccg cgcggcaaag tccgcgccca gcagcacgct cacgtcgact
     5641 agcctcgtcg aatcgggctg cagcaccacc gttcccacct gcagcgcgcg acggacgcgc
     5701 tcccccgcgg cccggtcccc ccgccgcacc aggatgcgcg tggaatccga gcgcgcgggc
     5761 agcggcacgg cgttgccgta gccgacgaca tcgatcccgg cctggcgcaa cacgagggtc
     5821 ccggtccggg ccagaccggg ccgccgggtg acgttgagga cctcgacgac gatgcgcggg
     5881 ccatcctggc ccggcacggc gaaggccggc cgcgcctcgt cgtgcccgca gcccgcgcag
     5941 aggagcagcc cgacgatcag gccgagacga gcgcgttcca gaaggccacc gtctccggtt
     6001 tcaggtccca cgacgatcgc aggacccact cgatccggtg ccgtgccact tcgtgcagca
     6061 ccgcgccgca ttcctccggc atgcgctccc gcaacgcggt actccgcgcg tcgtcgaagc
     6121 ggcggccggg ctcgaggtag tcggcgacat acagcatccg tccgacgtcg tcccacccag
     6181 caaagcccag cgagtggtag cgcacggcgt cgagcacgcc ctggtcccgc tccccgtccc
     6241 gggccaggcg gtcggcgcac agcggtccgt ggtcgagctc ggtggcggcc ggcgcgtcgc
//
LOCUS       DBNGZW010000622         4207 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_57864, whole genome shotgun sequence.
ACCESSION   DBNGZW010000622 DBNGZW010000000
VERSION     DBNGZW010000622.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4207)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_57864"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(30..1130)
                     /gene="amrS"
                     /locus_tag="AC16JC_14630"
     CDS             complement(30..1130)
                     /gene="amrS"
                     /locus_tag="AC16JC_14630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HIR5165983.1"
                     /translation="MSRRAIPLLNDDAVPGGGTTLARQLDVRVRPGELVQSLPDGAVR
                     CVACGHRCLVRPGRRGICQVRFNRDGTLYVPTGYVAALQCDPVEKKPFFHVLPGSDVL
                     TFGMLGCDFHCGYCQNWLTSQALRDAAAGVLPSDVTAAGLVDLALRRGARLIASSYNE
                     PLITAEWAVEVFRAARPAGLRSAFVSNGNCTPEVLDYLRPWTDGYKIDLKAMSDRRYR
                     ELGGVLQHVLDAIRLVHERGFWLEIVTLVVPGFNDDAAQLRDAAEYIASVSPEIPWHV
                     TAFHQDYRMTQAANTTAAMLERACEVGAAAGLKFIYAGNLPGRVGRWEHTWCPDCGDL
                     LIERHGYTIRRQRVGADGNCPSCGRRIPGIWC"
     gene            complement(1176..1970)
                     /locus_tag="AC16JC_14635"
     CDS             complement(1176..1970)
                     /locus_tag="AC16JC_14635"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HIR5165984.1"
                     /translation="MESQQRMRLVALLIGLMVIAVAWDVVGGHRAAAPPPVPPSPPVA
                     SPQPPPTSGRSQTGSEIVPAESGGASSLDIVARADMRRRIRSSFPLTYLSIIVAESTD
                     SMLHRWDDRVDRPVHVYLARGKAANFRPEFLDAVRGAFARWGETGLPVRFDLAADSTD
                     AEVRFTWRTQFDIDRTGETDLEWDRDGHLQSAVVTIATFDPDGRPLGSDDVRVVALHE
                     IGHLLGLDHSPDSTDLMFAKAVVRDLSPRDIRTAQLLYALAPGSLR"
     gene            2062..>4207
                     /locus_tag="AC16JC_14640"
     CDS             2062..>4207
                     /locus_tag="AC16JC_14640"
                     /inference="COORDINATES: protein motif:HMM:NF015155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR5165985.1"
                     /translation="MIALPARLIIRFRWVVIAAWVAFAALLVPRAAHVQRVLAVRGGP
                     ERPTESSRASNLIDAALPSPFAEYLAIVVRGPVPVGDSRFIAVLDTLSAVATREPSVR
                     QVISTRTVDDSAFVSADRRTTFLLAALARERTGDITKDFLPALRDSLHASLRRLPAGA
                     GFELMVTGSAALDHDVRTISAEDTTAGERRVLPLTLLVLILAFGALVAATLPIMVGLL
                     AITIALGLLTVLAQFSTMSVFVLNITTMVGLGVGIDYSLLIVTRFREELNRGLSPVDA
                     AIRTIRTAGSAVMTSGLIVVVGFGALVTTPLVETRSVGIGGLLVVAVAVLLATTFLPA
                     TLTLLGRDIDRPRWLARPLARIHAPTGWERWARWLGHRPWRAIAVGAGVVGLLMLPLT
                     QIRIGLPARNWFPSGSESGQGLALLRSMGANGLILPTRVVVQLPLGGSALRGPWLFGL
                     KVLTDSLRRDPRVKDVRGVASLRPRMSSFQLAVFYSDPAGVRARYPKFFDAYLSDDNR
                     TTLIDVIAADSVSLTGMMALTRHTRALVTQGVRGLAGARMLVGGFAAASVDLQDDLLH
                     RFPGVVLLVLGVTALMLLVAFRSLLVPLKAVLLNCLSVGAAFGVTVLVFQHGYGGSLF
                     GLEGATEAIFVVVPVLVFATVFGLSMDYEVFLLTRVKEVFDKTGRNDHATMEGLSMTA
                     STITFAAAIMIIVFGVFAFSRVLAVQFLGFGLA"
BASE COUNT          579 a         1473 c         1450 g          705 t
ORIGIN      
        1 cgccgccgcc tgcagcaggg ccgccaggcc tagcaccaga ttcccggaat gcggcgtccg
       61 caggaggggc agttgccgtc cgcgcccacg cgctggcgcc ggatcgtgta accgtggcgc
      121 tcgatcagca ggtcaccgca atcggggcac caggtgtgct cccagcgtcc cacgcggccg
      181 gggagattgc cggcatagat gaacttcagc cccgccgccg cgccgacttc gcaggcgcgc
      241 tcgagcatcg ccgccgtcgt attggcggcc tgcgtcatgc gatagtcctg atggaaggcg
      301 gtcacgtgcc acgggatttc gggagatacc gacgcgatgt actcggcggc gtcgcgcagc
      361 tgggccgcgt cgtcgttgaa gccgggcacg accagcgtca cgatttccag ccaaaacccg
      421 cgctcgtgca cgagtcgaat ggcgtcgagc acgtgctgca ggacgccgcc cagctcccgg
      481 tagcggcggt cgctcatggc tttgagatcg atcttgtagc cgtcggtcca gggccggagg
      541 taatcgagga cctccggcgt gcaattgccg ttcgagacga acgccgagcg cagcccggcg
      601 gggcgcgcgg cccgaaacac ctccaccgcc cattccgcgg tgatcagcgg ctcgttgtaa
      661 gaggaggcga tcagccgcgc gccgcgccgc agcgccaggt ccaccagccc ggcggccgtg
      721 acatcgctgg ggagcacgcc ggccgccgca tcccgcagcg cctgggaggt gagccagttc
      781 tggcagtatc cgcagtgaaa gtcgcagccc agcatcccga aggtgagcac gtcgcttccc
      841 gggagcacgt ggaagaacgg cttcttttcc acgggatcgc actgcagggc ggcgacgtag
      901 cccgtgggca catagagcgt cccgtcgcga ttgaagcgga cctggcagat gccgcggcgc
      961 cccggtcgca cgaggcagcg gtggccgcag gcgacgcacc gtacggctcc gtccgggagc
     1021 gattgcacca actcccccgg acgcacacgc acgtcgagct ggcgggcgag cgtcgtgccg
     1081 ccaccgggaa cggcgtcgtc gttcagcagg gggatcgcgc ggcgggacat gcggcaggct
     1141 ccggtgggga atataccctg gagcgcgccg ccctcctacc gcaggctgcc gggggccagg
     1201 gcgtagagga gctgagcagt ccggatgtcg cgcggcgaca gatcccgcac taccgctttg
     1261 gcgaacatga gatccgtgga atcgggcgaa tgatcgagcc cgaggaggtg gccgatctca
     1321 tgcagcgcca cgacccggac gtcgtcgctg ccgaggggcc gcccgtcggg gtcgaaggtg
     1381 gcgatcgtga ccaccgccga ctgcaggtgg ccgtcccgat cccactcgag atccgtttcc
     1441 cccgtccgat cgatgtcgaa ctgcgtacgc caggtgaacc ggacctcggc gtcggtggag
     1501 tccgccgcaa ggtcgaatcg gaccgggagg ccggtttccc cccagcgggc gaaggctccg
     1561 cgcactgcat ccagaaactc ggggcggaag ttggcggcct tgccgcgcgc caggtacacg
     1621 tgcaccgggc ggtcgacccg atcgtcccag cgatgcagca tggagtcggt cgattcggcg
     1681 acgatgatgc tgaggtaggt gagggggaag ctggagcgga tgcgccgccg catgtcggca
     1741 cgcgccacga tgtccaacga gctcgccccg cccgactcgg cggggacgat ttcgctcccg
     1801 gtctggctgc gccccgaggt cggcggcggc tgaggactcg ccacgggcgg cgacggcggc
     1861 accggcggcg gagccgcggc acgatgtccg cccacgacgt cccacgccac ggcgatcacc
     1921 atcaggccga tgagcagggc gaccaggcgc atgcgctgct gcgattccat gcctgcaaat
     1981 atgtccgccg tccgagccgc gccgctagct ttgccctgcg gcgtggtcta tgattgtggc
     2041 ctgcttatgg ttggacgctg aatgatcgca ctccccgccc gcctgatcat tcggtttcgc
     2101 tgggtggtga tcgcggcatg ggtggcgttt gcagcgctgc tcgtgccgcg ggccgcgcat
     2161 gtgcaacgcg tcctcgccgt gcgcggcggt cccgagcgcc ccaccgaatc gtcacgcgcc
     2221 tccaatctga tcgacgccgc gcttcccagt cccttcgccg aatacctcgc cattgtggtc
     2281 cggggacccg tgccggtcgg cgactcccgt ttcatcgccg tcctggacac gctctcggcc
     2341 gtcgcgaccc gcgagccgtc ggtgcgtcag gtcatctcca cgaggaccgt cgacgattcg
     2401 gcattcgtga gcgccgaccg ccggacaacg ttcctgctcg ccgccctcgc ccgcgagcgg
     2461 accggcgaca tcaccaagga tttcctcccc gcgctgcgcg acagcctcca tgcctcgttg
     2521 cgccgcctgc cggcgggtgc cgggttcgag ctcatggtca ccgggagcgc ggccctggac
     2581 cacgacgtgc ggacgatcag cgcggaagac acgacggcgg gcgagcggcg cgtgctgccg
     2641 ctgacgctgc tcgtgctgat cctcgcgttc ggcgcgctcg tcgccgcgac actccccatc
     2701 atggtcgggc tgctggccat cacgatcgcc ctgggtctgc tcacggtgct ggcgcagttc
     2761 tcgacgatgt cggtgttcgt cctcaacatc accacgatgg tcgggctagg cgtgggcatc
     2821 gactactcgc tgctcatcgt cacacgcttc cgcgaagagc tgaatcgcgg gctctccccg
     2881 gtcgatgccg ccatccggac catccgcacg gccggctccg ccgtcatgac gtcgggcctg
     2941 atcgtggtgg tcggcttcgg cgcgctcgtc acgacgccgc tcgtcgagac gcgctcggtc
     3001 gggatcggcg ggctgctcgt ggtcgccgtc gccgtcctgc tggcgaccac cttcctgccc
     3061 gccaccctga cgctgctggg gcgcgacatc gaccgccccc gctggctcgc gcggccgctg
     3121 gcccggattc acgccccgac cggatgggag cgctgggcgc gctggctcgg tcaccgtccc
     3181 tggcgcgcca tcgccgtcgg cgccggcgtc gtcggcctgc tcatgctccc cctcacgcag
     3241 atccgcatcg ggttgccggc gcgcaactgg tttccgtcag gctccgaatc gggtcagggc
     3301 ctcgccctgc tccgcagcat gggggccaac ggactgatcc tccccacccg ggtggtggtg
     3361 cagctcccgc tcggcggatc cgccctgcgc ggtccctggc tgttcgggct gaaggtgctc
     3421 accgattcgc tgcgccgcga cccgcgcgtg aaggacgtgc ggggcgtggc gtcgctcagg
     3481 ccgcgaatgt cgtcgtttca gctggccgtg ttctacagtg atcccgcggg cgtccgggcc
     3541 cggtatccca agttctttga cgcctacctg agcgacgaca accggaccac gctgatcgat
     3601 gtcatcgccg ccgacagcgt gtcgctgacc gggatgatgg cgctcacccg gcacacccgc
     3661 gcgttggtca cgcagggggt gcgcggcctc gccggggccc ggatgctggt cggcggtttc
     3721 gcggcggcca gtgtggactt gcaggacgat ctcctgcatc gcttcccggg ggtcgtactc
     3781 ctcgtgctgg gcgtcacggc gctgatgctg ctcgtcgcgt tccggtcgct gctcgtgccg
     3841 ctcaaggcgg tgctgttgaa ctgtctctcg gtgggcgcgg cgttcggcgt gacggtcctc
     3901 gtctttcagc acgggtacgg cgggtcgctg ttcggcctcg agggggcgac ggaggcgatc
     3961 ttcgtcgtcg tcccggtgct cgtattcgcc accgtgttcg gattgtccat ggattacgag
     4021 gtcttcttgc tgacccgcgt gaaggaggtc ttcgacaaga ccggccgcaa cgatcacgcg
     4081 accatggaag ggctctcgat gacggcatcc accatcacgt tcgccgcggc catcatgatc
     4141 atcgtgttcg gcgtgttcgc cttctcacgc gtgctcgccg tgcagttcct gggcttcgga
     4201 ctggcca
//
LOCUS       DBNGZW010000623         2128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1627854, whole genome shotgun sequence.
ACCESSION   DBNGZW010000623 DBNGZW010000000
VERSION     DBNGZW010000623.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2128
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1627854"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2128)
                     /locus_tag="AC16JC_14645"
     CDS             complement(<1..>2128)
                     /locus_tag="AC16JC_14645"
                     /inference="COORDINATES: protein motif:HMM:NF022481.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein O-mannosyl-transferase family"
                     /protein_id="HIR5165986.1"
                     /translation="NPLFTMMAHVWGMLPLAAAYAQRINLFAATTSAVAAACWFLVGE
                     RWMRPIVPVPLVRRLAALGGALVGATAFTVWNQSVVNEKVYTLSLLSIALILWLIVRW
                     DDQDPGEAHDHHLLLIMYLLALTSTNHMMGVLVGPVVIVLLFPPLMTRRAPTDDGRAM
                     EWSQFFAFVAVFAVLMGSGMEKATYMIPGAILFVLALGYALYRGDWRFTLALLAVAAV
                     GVSVYIFLPLRAAHYPPINEGEPTTWQALWAVLTRQQYGKPPLTQRQADIVSQVGLWV
                     QYFGWQWAHDLQLKIQASIAVIFGGLGVLGAVRHFKADRRHAIAMISLMATLTLLLII
                     YLNFKYGYSQHYGRQLAREVRERDYFFVCSFALWGIWVGFGLATLMEWVQDGLRDRLD
                     DEAARWRLALPILLVALVPLAFNRLTASRAHETLARDFAVDLLNSVEPYGILVTAGDN
                     DTFPLWYAQEVEGVRRDVVIINLSLANTDWYLRQMQRRPVEEYDAARGPAIYRDRVWP
                     KPTGKVLAFTAGQLDSLELYYPLDQKRALNLGGVPVVLDPEQIHPLGNGQGYLERADV
                     VVLGAMRDQLGKRPIYFSRTVGTYADEFGLTPYLEGQGFARALRTQPLQASDSIVALP
                     GFGWVNLARSKALAFDVYHVSAAARQRPRGWVDVPSEGILVTYALIYQVLGQALQPKD
                     PALATRAAAIADSITKNSANFRSALSP"
BASE COUNT          376 a          688 c          768 g          296 t
ORIGIN      
        1 ggcgaaagcg cgctgcggaa attggcgctg ttcttggtga tcgagtccgc gatcgcggca
       61 gcgcgggtgg cgagggcggg atcctttggc tgcagggcct gacccagcac ctgatagatc
      121 agcgcgtagg tcacgagaat gccctcggag ggcacgtcca cccagccacg gggccgctgc
      181 cgggcggcgg cggacacgtg gtacacgtcg aaggcgagcg ccttggagcg ggcgagattg
      241 acccagccga agccggggag cgctacgatg ctgtcgctcg cctgcagggg ctgggtgcgc
      301 aacgcgcgcg cgaatccctg gccctcgaga tagggggtca gcccgaactc gtcggcgtac
      361 gtgcccacgg tgcgcgagaa gtagatcggg cgcttgccga gctgatcgcg catcgcgccg
      421 agcaccacca catcggcccg ctcgaggtat ccctggccgt tgccgagcgg gtggatctgc
      481 tcggggtcga ggaccaccgg cacgccgccc agattgagcg cccgcttctg gtcgagcggg
      541 tagtagagct cgaggctgtc caactgaccc gcggtgaacg cgagcacctt gccggtgggc
      601 ttgggccaga cgcggtcgcg gtagatggcg gggccacggg cggcgtcgta ctcctcgacg
      661 ggacgtcgct gcatctggcg caggtaccag tcggtattgg ccagtgacag gttgatgatg
      721 accacgtcgc gccgcacgcc ttcgacctcc tgcgcgtacc acagggggaa ggtgtcgttg
      781 tctcccgcgg tgacgaggat gccgtagggc tcgacggagt tcagcaggtc caccgcgaag
      841 tcgcgcgcca gcgtctcgtg ggcgcggctg gcggtgaggc ggttgaaggc gaggggcacc
      901 agcgccacca ggaggatggg gagcgccagg cgccagcgcg cggcctcgtc gtcgagccgg
      961 tcgcgcaggc cgtcctgcac ccattccatc agggtggcga ggccgaagcc gacccagatg
     1021 ccccacaggg cgaacgaaca gacgaagaag taatcgcgct cgcgcacctc gcgggccagc
     1081 tgccggccgt agtgctgcga gtagccgtac ttgaaattga ggtagatgat gagcagcagc
     1141 gtgagcgtag ccatgaggga gatcatggcg atcgcgtgac ggcggtcggc cttgaaatgc
     1201 cgcaccgcgc ccagcacgcc gagaccgccg aagatcaccg cgatgctcgc ctggatcttc
     1261 agctgcaggt catgggccca ctgccatccg aagtactgca cccacaggcc gacctgcgag
     1321 acgatgtccg cctggcgctg ggtgaggggc ggtttgccgt actgctggcg cgtgagcacg
     1381 gcccagagcg cctgccaagt cgtcggctcg ccttcgttga tgggcgggta gtgcgccgcg
     1441 cgcagcggca ggaagatgta gacggagacg ccgaccgcgg ccacggccag caacgcgagc
     1501 gtgaagcgcc agtcgccccg gtacagggca tagcccaggg cgaggacgaa caggatggcg
     1561 cccgggatca tataggtggc cttttccatc cccgacccca tcaggaccgc gaacaccgcg
     1621 acgaacgcga agaactgcga ccactccatg gcgcggccgt cgtcggtcgg cgcccggcgc
     1681 gtcatcagcg gcgggaagag cagcacgatc acgaccggcc cgaccagcac acccatcatg
     1741 tgattggtgg acgtgagcgc gaggaggtac atgatgagca acaggtgatg gtcgtgcgcc
     1801 tctcccggat cctggtcgtc ccagcggacg atgagccaca ggatcagcgc gatcgacagc
     1861 aggctcagcg tgtacacctt ctcgttcacg accgactggt tccacacggt gaacgccgta
     1921 gcgccgacga gggcgccgcc cagggcggcg agccggcgca ccagcggcac gggcacgatc
     1981 ggacgcatcc agcgctcgcc caccaggaac cagcaggccg ccgcgaccgc gctggtcgtg
     2041 gccgcgaaca ggttgatgcg ctgcgcgtac gcggcggcga gcggcagcat cccccacacg
     2101 tgcgccatca tcgtgaacag cgggttgc
//
LOCUS       DBNGZW010000624        11158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1408076, whole genome shotgun sequence.
ACCESSION   DBNGZW010000624 DBNGZW010000000
VERSION     DBNGZW010000624.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 11158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11158
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1408076"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1574)
                     /locus_tag="AC16JC_14650"
     CDS             complement(<1..1574)
                     /locus_tag="AC16JC_14650"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR5165987.1"
                     /translation="MKRVWCLTGALLAVLWMAPPLGAQQSTGTIRGRVTEAGTQQPIS
                     GVHISIGTRGVLSQPDGRYVLAGVPAVTDSLRARIIGYSPTAQIVTVAGGDTIVVDFA
                     MTAQAVGMSEVVVVGYGQQTLGNITGAVTAVTDSQFNTGRVISPQMLIQSKVAGVQVV
                     DNNDPGGGLSIRIRGATSVNASSEPLYVIDGVPLGTGAGGGLSAGRDPLNFLSPNEIE
                     SITVLRDASAAAIYGANAANGVVLITTKSGRGREGVQVEYNSSASASSVTRLPDMLNA
                     SQFAAAVAQYAPSRVDSLLGANTNWLSLIDRTGYGQEHNLSVSNEGGGMSYRLSLGYL
                     NQKGIVQASSTERVTLGVNYRQRIFSDRLSLRANVRGSRALDRFTPGDVLGNAAAMAP
                     TQPVLDPTSPTGYWDWRTTNASPSNPLASLNLATDHGTTWRSVGNALAEYRLPFLEGL
                     SANLNLGYDLTQADRTTFNPSNLAAQIRQGHGMLYLANNTQTNSVLDAYLGYAPTRSY
                     GPGTIDLVGGYSYMQS"
     gene            complement(1737..3008)
                     /locus_tag="AC16JC_14655"
     CDS             complement(1737..3008)
                     /locus_tag="AC16JC_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA/RhaT family transporter"
                     /protein_id="HIR5165988.1"
                     /translation="MDRLSDLDPARLRQLQRVGTLSGFAAGAWLGAAEAPTKLVTLGI
                     SPVVISFIMVLGVFLARWSLPALVRGTAALKDDVRQAPHLLVWAALGGCLWAVANTLT
                     IFAIKDIGLAIAFPLWNSNSLLGILWGTLLFNELRDAGPARWAGVLGGALAMFAGATV
                     LAVTSSAHAPGGNAFRGIVAALTAGALWGTMYIPYRKAYLTGMHPLSFVAFFTIGELT
                     MMTVLALTFLGGPAALGHELAGSRGIFFWLLVAGFVWVVGDLFQQYAAKYVGISRGIP
                     LSNTNQLWGLLWGMLVFGELAGGGRGAVIAGSLLMAAGAGAIALASAPGREYRRWQEA
                     GARESARYGVDPVYAAARMAGDDASTAARRRPLDWLLVLAATAVFVGSAALARVPALQ
                     IGWGWAAALTLALFGFLVAGGVALWRTTRFG"
     gene            complement(2996..3106)
                     /locus_tag="AC16JC_14660"
     CDS             complement(2996..3106)
                     /locus_tag="AC16JC_14660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="translocated intimin receptor Tir"
                     /protein_id="HIR5165989.1"
                     /translation="MPPTPRSRWHALVGDVHFWIPVAVLVLGLLVLWWIA"
     gene            3259..3984
                     /locus_tag="AC16JC_14665"
     CDS             3259..3984
                     /locus_tag="AC16JC_14665"
                     /inference="COORDINATES: protein motif:HMM:NF007291.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HIR5165990.1"
                     /translation="MKILYCDDYESVSLQAASLVIAEITQKRSLLLCAATGNSPIGLY
                     DRLVRKAATDRALFAELRIIKLDEWGGIPENAPGSAERYLRTRLLDPLGIAPERYISF
                     ASTPAEPADECERIRSELERHGPIDVCVLGLGVNGHIGFNEPGPFLMPQCHVARLSAE
                     TRRHAMVQSLDTQPLFGLTLGMQEILAARRIILLVAGAGKQQAVAGVLSGAVSTTLPA
                     SLLWLHGCVDCLIDRHAVANHAP"
     gene            4100..4579
                     /locus_tag="AC16JC_14670"
     CDS             4100..4579
                     /locus_tag="AC16JC_14670"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR5165991.1"
                     /translation="MTSFASRALLPVGLVMGIVSGCARAHGKGQGDMADTTTAAPSPP
                     SATVTSEDIQRTPTMSIEQLLMSRFPGVEVTRTADGGISLRIRGRTSILASNDPLYVL
                     DGLPIQPGPNGSLSGINPYDIESIDVLKDAASTAMYGGRGANGVIVIKMKRPPPRSP"
     gene            complement(4571..5665)
                     /locus_tag="AC16JC_14675"
     CDS             complement(4571..5665)
                     /locus_tag="AC16JC_14675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5165992.1"
                     /translation="MPSVTFDHVSKQFGATRVVEDFDLRVADGELLVLVGGSGSGKST
                     ILRMLAGLEHVTSGTIRIDDRDVTVLPARERDVAMVFQDYALYPHMTVRENLSLGLRL
                     RKVPRPEIDRRVEWAASILGLGPLLERKPKQLSGGQRQRVAMGRAIVREPSVFLFDEP
                     LSNLDAGLRAQMRIEIGALQRRLNTTTVYVTHDQVEAMTLGDRIVVLADGRIQQVGEP
                     IELYRAPVNRFVAGFIGTPRMNFVEGRLEADLGAATFIADAARVKLTRPLSMAPGSSA
                     AATLGVRPEDLRLSTPDSSERLASDTLPGRVVLVERLGGTSHVHVDLGAHRLIASVAD
                     GAVPHIGDDVAVRIPSDRAYLFAADGRALR"
     gene            complement(5665..6480)
                     /locus_tag="AC16JC_14680"
     CDS             complement(5665..6480)
                     /locus_tag="AC16JC_14680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020537097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5165993.1"
                     /translation="MKTLGRVALYLALTLAALTFIYPFLWMASTTLKPPIEVGTLALV
                     PDHVTLDNYRTMWARAPFGRALLNSVLVATTITAAVLVFGSMTAYAIARLRFRGRPAL
                     NAATMAVLLVPGQLTLIPLYTLIVQLGWIDTYAALIVPYLFNATAILMLRQFFLQIPQ
                     SLIDSARMDGMSELRILFTIFWPLSRPVLAIVAIFTFMGSWNEVLWPLLVVRDQHLMT
                     LPQLLTVFALGGGAGTMGVSLASAMVLVVPVVVAYAFLQRYFIESLAGAGIKG"
     gene            complement(6477..7340)
                     /locus_tag="AC16JC_14685"
     CDS             complement(6477..7340)
                     /locus_tag="AC16JC_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012842875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5165994.1"
                     /translation="MRAEERSGWLLAAPYSAFLAAFALYPIAFALVLVFLKWDLVTAP
                     AFAGLDNVRLLAVDGRFWQAVVTMFVFFGIHIPLQIVAALGLAMALNRPLALRAFWRA
                     AFFLPVVISGAVVAILWSNLYATDVGLINHLLVRLGVRPVAWLTDPVTAMPAIAVMVT
                     WKNVGFYVIIYLAGLQYVPRSCQEAIEMEGATAWQRFRHLTLPSLLPQTVLVVTLSTI
                     NGFQLFIEPYVMTGGGPLRRTYSLVLYLYTNAFSYQKMGYAATIGVALALIIGVVVVL
                     QRRLIGTADAQ"
     gene            complement(7330..9942)
                     /locus_tag="AC16JC_14690"
     CDS             complement(7330..9942)
                     /locus_tag="AC16JC_14690"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR5165995.1"
                     /translation="MIAARPQIVARALALATLVGGGGCGRDGRPVAEDASPPPVTISL
                     GHLRHLGLDAVVAGKPVRVVALYAEAPDYRPTPSPMRDGSEGIASVDDAARAAVLYLR
                     VYEATGDSLARDEALGLLAFVTAMEQGDGEFVNFIDARGRPNRTVPSGRKSMSYWAAR
                     GIWAMGEAVRVLGKERPDRLIHVRPVLGRAVARMTREVNAHRLIGGSATATAEALLGL
                     LALQRAEPSPALAALAAGTAELLVPLAAGTETAAPWGAHVDRAGAAWHAWGARSTQAL
                     ADAAVVLARPDFVLAAQREADALWGRFLLAGQPASSLSRDGAATWFPQIAYGVGPIVE
                     GYLALAEATGERRYSVLAGLAAGWFVGDNAAGVSMYDETTGRTFDGIDGPTAGQVNRN
                     AGAESTIEALLALQSVTSDAEAAPYLRYRAAGQRATSLDSIPDSREFVGPTGERLVLR
                     RRPAGLEIHESSPPGGPITLTYWPAANPTETRLATRLVASWNAEHPDIQVRVQPLPAG
                     RSSEEVLLAAIVAHATPDVSSNVSSALLARLVRAHGVVRLDNRASTAARLRERTRPTM
                     LASLRLPDSGIYAFPWKTNPEMLMYNVDLLAAAGVRPPRTQSELLEAFRRLTRDTDGD
                     GRTDHWALWATLKTTWFERFYDFYPLYLASSGGVTVVQGGTVVFDNRAAVSALDVLRR
                     GFAEGTLPRSNFALGRDPFADGTVAMKIIGPWFVKELDELKVPGLRYDVVPVPPADGT
                     SPDDAFAFADLRSIAVFSTTRHPDAAARFVAYLTSPAADRLLIEEASQLPYRRALASD
                     PRFVTALARWPTLATYATYVERTRDIDIDPDVVELFDLLSEAYEVGAVYGTMSVQQAL
                     TNAAAEARKILDAR"
     gene            complement(9939..10925)
                     /locus_tag="AC16JC_14695"
     CDS             complement(9939..10925)
                     /locus_tag="AC16JC_14695"
                     /inference="COORDINATES: protein motif:HMM:NF015971.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 130 protein"
                     /protein_id="HIR5165996.1"
                     /translation="MIAPLERLASNPLITPAQIPFMRATGSFNPGATIDRASGRVVLL
                     VRVFEEQARRSCLALALSADGERIDDIWAHPAFAREAPYEEWGVEDPRITYLEDDRRY
                     AITYTGYSRDGPRVCLITTDDVLTPGRYRRHGPRIAGDNKNCVIFPQKIDGDYVILHR
                     PMPRMVCARVRSLDDSWPADGIALVGPRAGTWRSARVGAGAPPIRTEIGWLLPVHGAT
                     AIDEGNVYSMGWCVLDLHRPETVKYVSDEPALAPQAPYEIAMQHIPQVDPANFPTGVR
                     VVFPQGLVERGDDLLVYYGAADVSVAGARVNKHALVDSLRAAIAANQGGVAL"
     gene            complement(10922..>11158)
                     /locus_tag="AC16JC_14700"
     CDS             complement(10922..>11158)
                     /locus_tag="AC16JC_14700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5165997.1"
                     /translation="ATARRERSFGLVRADGSEKHAVRVFRAFKARRDAGTLPTSPVPA
                     LLDVSADEYYRTPASHFARLYATWLERTPGPLPS"
BASE COUNT         1968 a         3723 c         3813 g         1654 t
ORIGIN      
        1 tgcgactgca tgtaggagta gccgccaacc aagtcgatgg tgccgggtcc gtaggaccgt
       61 gtcggcgcgt agcccaggta cgcatcgagc accgagttcg tctgcgtgtt gttcgccaga
      121 tagagcatgc catggccctg ccgtatttgg gcggccaggt tgctcgggtt gaacgtcgtt
      181 cgatcggcct gcgtcaggtc gtaacccaag ttgagattgg cggacaaccc ctcgaggaac
      241 ggcagacggt actcggccag cgcgtttccc acgctgcgcc aagtcgtccc gtggtccgtg
      301 gccaggttca ggctggcgag gggattgctc ggagacgcat tcgtcgtcct ccagtcccag
      361 taccctgtcg ggctggtcgg atccagcacc ggttgtgtcg gcgccatcgc ggcggcattg
      421 cccagcacgt cgcccggtgt gaaccggtcc agggcacgcg agccccgcac gttggcgcgc
      481 aggctcagac ggtcgctgaa gatgcgctgc cggtagttga ccccgagcgt gacccgttcc
      541 gtcgagctcg cctgaacaat gcccttctgg ttgaggtagc cgagcgacag gcggtaactc
      601 atgccgccgc cctcattgct gacggacagg ttgtgttcct gaccgtaccc ggtgcggtcg
      661 atgaggctga gccagttggt gtttgcccca agcagcgaat cgacccgcga gggcgcgtac
      721 tgggccactg ccgcggcgaa ctgggatgcg ttcagcatgt ccggcagcct cgtgacggac
      781 gaggcggacg cgctggaatt gtactcgacc tgtacgccct cgcggcccct tcccgacttc
      841 gtggtgatga gcacgacgcc gtttgcagca ttggctccgt agatcgcagc ggccgaggca
      901 tcccgaagca ccgtgatgct ctcgatctcg ttggggctga ggaaattgag tggatcgcgc
      961 ccggcagaga gaccgccacc cgcgccagtg cccagaggca cgccgtcgat gacgtacagc
     1021 ggctcgctac tggcattcac cgaggtcgcg ccgcggatcc gaatcgagag accaccgcct
     1081 gggtcgttgt tatccactac ctgtacgccg gcgaccttgc tctggatgag catctggggg
     1141 ctgatgacgc ggccggtgtt gaactgcgag tcggtcacgg ccgtgacggc cccggtgatg
     1201 ttccccagag tctgctgccc gtagcccacc accaccacct ccgacatgcc gaccgcctgc
     1261 gcagtcatcg cgaaatcgac gacgatcgtg tcaccccccg cgaccgtcac gatctgcgcc
     1321 gtgggcgagt acccgatgat cctcgcccgc aggctatccg tgacagccgg gacccccgca
     1381 agcacatagc ggccgtccgg ctggctcagt acaccgcggg ttccaatgga gatgtggacg
     1441 cccgaaatcg gctgctgcgt ccccgcttcg gtcacccgac cacgaatcgt gccggtggac
     1501 tgttgagcgc caagtggtgg tgccatccag aggacggcga ggagggcacc ggtcagacac
     1561 catacccgtt tcatggcatg tctcctcgat ctgtgaaggt ttctttaacc cgaggtgatg
     1621 tgctaacgct cggaaggaag tctgtcaacg acctgcctgg gcattaccca tccttcatac
     1681 tgcctggagg tgtgtgaagg aagccatatg tctacatgaa gggatcacct caagtctcac
     1741 ccgaaccgcg tcgtccgcca cagcgccaca ccgcccgcga ccagaaatcc gaagagcgcg
     1801 agcgtgagcg ccgcggccca tccccagccg atctggagcg cgggcacgcg ggcgagcgcg
     1861 gcggacccca cgaacaccgc ggtcgcggcg aggacgagca gccagtcgag cggcctgcga
     1921 cgggcggccg tggatgcgtc gtcaccggcc atccgggccg ccgcatagac gggatccacg
     1981 ccgtaccggg cgctctcccg cgcccccgcc tcctgccaac ggcggtactc gcgaccgggc
     2041 gccgaggcga gagcgatcgc ccccgccccc gcggccatca agagcgagcc cgcaatcacg
     2101 gcaccccggc cgccgccggc cagctcgccg aacacgagca tcccccacag gagtccccac
     2161 agctgattgg tgttcgagag cgggatgccg cggctgatgc cgacgtactt ggccgcgtac
     2221 tgctgaaaca ggtcgcccac cacccatacg aagcccgcga cgagcagcca gaagaagatt
     2281 ccccggctgc cggccagctc gtgccccagc gcggccggtc ccccgaggaa ggtgagggcc
     2341 agcaccgtca tcatggtgag ctcaccgatc gtgaagaagg cgacgaacga gagcggatgc
     2401 atgccggtga ggtacgcctt gcggtacggg atgtacatcg tgccccacag ggcgccggcc
     2461 gtgagcgcgg ccacgatgcc gcggaacgca ttgccccccg gcgcatgagc ggacgacgtc
     2521 acggccagca ccgtggcccc ggcgaacatg gcgagcgcgc cgcctagaac gccggcccag
     2581 cgagctggtc ccgcgtcccg cagctcgttg aacagcagcg tgccccagag gatgccgagc
     2641 aggctgttcg agttccacaa cgggaacgcg atggcgaggc cgatgtcctt gatcgcgaag
     2701 atcgtgagcg tgttggccac ggcccacagg caccccccga gcgccgccca gaccagcaag
     2761 tggggagcct gccgaacgtc gtccttgaga gcggcggtgc cgcgcaccag cgccgggagg
     2821 ctccagcgcg cgaggaacac ccccagcacc atgatgaacg agatcacgac gggcgagatg
     2881 ccgagggtga cgagcttcgt gggcgcttcg gccgcgccca gccaggcacc cgcggcgaac
     2941 ccggagaggg tacccacccg ctgcagctgg cgcaggcgcg cagggtcgag gtcgctcagg
     3001 cgatccacca gagtacgagg agcccgagca ccaggacggc cacgggaatc cagaagtgga
     3061 cgtcgcccac gagggcgtgc cagcgggagc gaggtgtggg gggcacggga cagaaacaat
     3121 gacggtgaaa cggatcgctc gctagggccg gcgcggcgcc cttgcccggc gctggcggga
     3181 gccgagcgcg gtgcgtatga tggccaaaca ccggacggaa ggtaccggcc ttacaacctc
     3241 tggaagaagt gacctggagt gaagattctc tactgcgacg attatgaaag cgtaagcctc
     3301 caggcggcct cgctcgtcat cgccgaaatc acacagaaac gaagcctgtt gttgtgcgct
     3361 gcgaccggca actcgccgat cggcttgtac gatcgtctgg tgcggaaggc agcgaccgac
     3421 agagcgctct ttgcagagct cagaataatc aagttggatg agtggggcgg tattcccgag
     3481 aatgctccgg gatcggccga gcggtacctg agaacgcggc tgctggatcc attggggata
     3541 gcgccggaac gctacatatc atttgcctcg acgcccgcag agcctgctga cgaatgcgag
     3601 cgcattcggt cggagctgga gcggcacggg ccaattgatg tgtgcgtatt gggacttggt
     3661 gtcaacggtc acatcggatt caatgagcct gggccgtttc tgatgcccca gtgtcacgtg
     3721 gcgcgcttgt cggcggaaac acgtcggcac gccatggtgc aatcactgga tacccagccg
     3781 ctcttcgggc tgaccctcgg catgcaggag atactggcgg cgagaagaat catcctgttg
     3841 gtggccggtg cagggaagca gcaagccgtt gccggggtgt tgtcgggagc agtgtcgacg
     3901 acattgccgg cctccctgct ctggttgcat gggtgtgtcg attgtctgat cgatcggcac
     3961 gcagtcgcca atcatgctcc ttgacggggg ctcgtaacat ggcgtcttcc ttggctgaca
     4021 ccgcgccggg ccacggccta tattgcgaca gagcctgcgg ttgggtatgg gcccctgctg
     4081 accgccccag gaggttccaa tgacctcgtt cgcttcgcgc gcgctgctgc ccgtcggcct
     4141 cgtgatggga atcgtctccg gatgcgcccg tgcccatggg aagggtcagg gggacatggc
     4201 ggacaccacg acagccgcgc cgtccccgcc ctccgcgacc gtgacctcgg aggacatcca
     4261 gcgcacgccc accatgtcga tcgagcagct cttgatgagc cggttccccg gcgtcgaggt
     4321 cactcggacc gccgacggcg gcatctcgct tcgtatccgc ggcaggacct cgattctcgc
     4381 cagcaatgac ccgctctatg tgctggacgg cctcccgatc cagccgggac cgaatggaag
     4441 cctcagcggg atcaatccgt acgacatcga atccatcgac gtcctcaagg atgccgccag
     4501 cacggcgatg tacggcggcc gcggcgccaa cggcgtgatc gtgatcaaga tgaagcgtcc
     4561 cccgccccgt tcaccgtaac gctcggccgt ccgccgcgaa caggtacgcg cggtcgctgg
     4621 ggatgcgcac cgcgacatcg tcgccaatgt ggggcacggc gccgtccgcg accgaggcga
     4681 tgagtcggtg cgcgccgagg tcaacgtgca cgtggctggt cccgccgagc cgctcgacga
     4741 ggaccacgcg cccaggcagg gtgtcggacg ccaggcgttc agacgaatcc ggcgtggaga
     4801 gccgaagatc ctcgggacgg acgccgagcg tcgccgcggc ggacgatccg ggtgccatgg
     4861 acaacggtcg cgtgagcttc acgcgagcag cgtcggcgat gaacgtcgcc gcgccgagat
     4921 ccgcctcgag ccgtccctcg acgaagttca tccgcggtgt gccgataaag ccggcgacga
     4981 agcggttgac gggagcgcga tagagctcga tcggctcgcc gacctgctgg atgcgcccgt
     5041 ccgccagcac cacgatgcgg tcgcccaggg tcatcgcctc tacctgatcg tgcgtgacgt
     5101 agacggtcgt cgtgttcagg cggcgttgca gtgcgccgat ctcgatgcgc atctgcgcgc
     5161 gaaggccggc gtccaggttc gacagcggct catcgaagag aaataccgac ggctcgcgca
     5221 cgatcgcacg tcccatcgcg acgcgctgcc gctggccgcc ggacaactgc ttcggcttgc
     5281 gctccagcag cggcccgagc cccaggatcg acgccgccca ctcgacgcgc cggtcgatct
     5341 ccggccgcgg tactttccgc agccgcagcc cgagcgagag attctctcgc accgtcatgt
     5401 ggggatacag cgcgtaatcc tgaaagacca tcgccacgtc gcgctcgcgc gcggggagta
     5461 cggtcacgtc ccggtcgtcg atacggatcg tgccgctcgt cacgtgctcg agtcccgcca
     5521 gcatgcggag aatcgtggat ttgcccgagc cggagccccc gaccagcacc aacagctcgc
     5581 catcggccac ccggaggtcg aaatcctcga cgacgcgcgt ggcgccgaac tgcttggaga
     5641 catgatcgaa cgtcacggag ggcattatcc cttgatcccc gcgccggcca ggctctcgat
     5701 gaaatagcgt tgcaggaacg cgtacgcgac gaccaccggc accaccagca ccatggccga
     5761 ggcgagcgac actcccattg tgccggctcc gccgccgagg gcgaacacgg tgaggagctg
     5821 ggggagcgtc atgagatgct ggtcgcgcac gaccagcaac ggccacagca cctcattcca
     5881 cgatcccatg aaggtgaaga tggctacgat ggcgagcacc ggccgcgaga ggggccagaa
     5941 gatcgtgaag aggatgcgca gctcgctcat cccgtccatg cgcgccgagt cgatgagcga
     6001 ctgcggaatc tggagaaaga actgccgcaa catgagaatc gccgtcgcgt tgaacagata
     6061 ggggacgatg agcgcggcat aggtatcgat ccacccgagc tgcacgatca gcgtgtacag
     6121 cggaatgagc gtgagctgtc ccggaacgag gaggaccgcc atcgtcgcgg cgttgagcgc
     6181 ggggcgcccg cgaaagcgca ggcgagcgat cgcgtacgcc gtcatcgagc cgaagacgag
     6241 aaccgccgcg gtgatcgtcg tggcgacgag cacgctgttg aggagcgcac gcccgaaggg
     6301 cgcgcgggcc cacatggtcc ggtaattgtc gagcgtcacg tggtccggga cgagcgcgag
     6361 tgtcccgacc tcgatgggcg gtttgagggt cgtggaggcc atccacagga aggggtagat
     6421 gaaggtgagc gcggcaagcg tgagcgcgag atagagcgca acgcgaccga gcgtcttcat
     6481 tgtgcgtccg ccgtgccgat gaggcggcgc tggagcacca cgacgacgcc gatgatcagc
     6541 gcgagcgcga cgccgatcgt ggccgcgtag cccatcttct ggtaggagaa cgcgttcgtg
     6601 tacagataca gcacgagcga gtacgtgcgt cggagcggcc cacccccggt catgacgtac
     6661 ggctcgatga agagttggaa gccgttgatc gtggagagcg tgaccaccaa caccgtctgg
     6721 gggaggagcg acggaagcgt caggtggcgg aagcgctgcc acgccgtcgc cccttccatc
     6781 tcgatcgctt cctggcagct gcgcgggacg tactgcaagc ccgcgaggta gatgatcacg
     6841 tagaagccga cgttcttcca cgtcaccatc acggcgatcg ccggcatcgc ggtcaccgga
     6901 tcggtaagcc aggcgaccgg gcgcacgccg agacggacga ggaggtggtt gatcaatccc
     6961 acgtcggtcg cgtacaggtt gctccagaga atcgcgacca cggccccgga gatcacgacg
     7021 gggagaaaga acgcggcgcg ccagaaggcc cggagcgcga gcggacgatt gagcgccatc
     7081 gccagcccga gcgccgcgac gatctgcagc ggaatgtgga tgccgaaaaa gacgaacatg
     7141 gtgacgacgg cctgccagaa gcgcccgtcg acggccaaca gccgcacgtt gtccagtccg
     7201 gcgaacgccg gcgccgtcac caggtcccac ttgagaaaga cgagcaccag ggcgaacgcg
     7261 atggggtaga gcgcgaacgc cgccaggaac gcgctgtacg gcgcagccag cagccagccc
     7321 gagcgttcct cagcgcgcat cgagaatctt cctcgcttca gctgcagcgt tggtcagcgc
     7381 ctgttgcacg gacatggtgc cgtacacggc gcccacttcg tacgcctcgg acaggaggtc
     7441 gaagagctcg acgacatccg ggtcgatatc gatgtcgcgc gtgcgctcca catacgttgc
     7501 ataggtggcg agcgtcggcc accgcgccaa tgcggtgacg aagcgcggat ccgaggcgag
     7561 ggcacgccgg taggggagct ggcttgcctc ctcgatcaac aggcgatccg cagcgggaga
     7621 cgtgaggtac gcgacgaagc gcgccgcggc gtcgggatgc cgcgtggtgg agaacacggc
     7681 gatgctccgc aaatccgcaa acgcaaacgc gtcgtccggc gacgtgccgt cggccggcgg
     7741 gaccggcacc acgtcatagc gtagtccggg caccttgagc tcatccagct ccttcacgaa
     7801 ccaggggccg atgatcttca tggcgaccgt gccgtccgcg aacgggtcgc ggcccaatgc
     7861 gaagttcgac cgcggcagcg tcccctccgc gaagccccgg cgcagcacgt ccagcgccga
     7921 cacggccgcg cggttgtcga agacgacggt acccccctgc acgaccgtca ctccaccgga
     7981 gctcgccagg tacagcggat agaaatcata gaagcgctcg aaccacgtgg tcttgagcgt
     8041 ggcccagagc gcccagtgat ccgtccgccc gtcgccatcc gtatcgcggg tgagccgccg
     8101 gaaggcctcg agcagctcgg actgcgtgcg cggcggtcga acgccggcag cggcgagcag
     8161 atccacgttg tacatcagca tctcgggatt cgtcttccac ggaaacgcgt agatccccga
     8221 gtccggaagg cgcagcgacg cgagcatcgt cgggcgtgtg cgctcgcgca ggcgtgccgc
     8281 ggtggacgcc cgattgtcca ggcgcacgac accgtgcgcg cggacgagcc gtgcgaggag
     8341 cgccgaggag acgttggagc tcacatccgg agtggcgtgc gcgacgatgg cggcgagcag
     8401 cacttcctcg gacgagcggc cggcgggcag cggttgcacg cgcacctgga tgtccggatg
     8461 ctccgcgttc cacgaggcga ccagccgcgt cgcgaggcgg gtctcggtcg gattcgccgc
     8521 cggccaatac gtgagcgtga tcggtccgcc ggggggagag gattcgtgga tctcgaggcc
     8581 ggcgggccgg cggcgcagca ccagtcgttc gccggtcgga cccacgaact cgcgtgaatc
     8641 cggaatgctg tcgagagacg tcgcgcgctg ccccgccgca cggtaacgga ggtacggtgc
     8701 cgcctcggca tcgctcgtga cgctctgcaa cgccagcagc gcctcgatcg tggattcggc
     8761 acccgcgttg cggttgacct ggcccgcggt cggcccatca atcccgtcga acgtcctacc
     8821 cgtcgtctca tcatacatgc tgacgcccgc agcgttgtct ccgacgaacc accccgccgc
     8881 caaacccgcg agcaccgagt agcgccgttc gcccgttgcc tcggcgagcg cgagataccc
     8941 ttcgacgatg ggcccgacgc cgtaggcgat ctgcggaaac cacgttgccg cgccgtcacg
     9001 ggagagcgac gatgcgggtt ggccggcgag caggaatctg ccccagagcg cgtccgcttc
     9061 gcgttgcgcc gccaggacaa aatcgggtcg cgcgagcacg accgcggcgt ccgcgagggc
     9121 ttgggtggag cgcgcgcccc aggcatgcca cgctgcgccg gcgcgatcga catgcgcgcc
     9181 ccagggcgca gccgtctcgg tcccggcggc gagcggcacg agcagctcgg cggttcccgc
     9241 ggccaacgcg gcgagcgcgg gcgagggctc ggcgcgctgc agcgcgagga gtcccagcaa
     9301 cgcttccgcg gtggcggtgg cggaaccgcc gatgagacga tgcgcattca cttcgcgcgt
     9361 catgcgcgcc acggcgcgcc ccagcaccgg cctcacgtgg atcagccggt ccggtcgttc
     9421 cttgccgagc acgcgcaccg cttcccccat ggcccaaatg ccgcgggcgg cccagtacga
     9481 catgctcttt ctgccgctgg gcacggtgcg attgggacgg ccgcgcgcgt cgatgaagtt
     9541 gacgaactcg ccgtcgcctt gctccatggc ggtgacgaac gccaggagtc ccagcgcttc
     9601 gtcgcgcgcg agcgaatccc ccgtcgcttc gtacacgcgc agatacagca cggcggcccg
     9661 cgcggcgtcg tccacgctcg caatcccctc ggacccgtcg cgcatcggtg acggcgtggg
     9721 tcggtagtcg ggcgcctccg cgtacagcgc cacgacgcga acgggcttgc ctgcgacgac
     9781 cgcgtccagc ccgaggtggc gcagatgccc gagcgagatg gtcactggag gcggcgaggc
     9841 atcctccgcc acggggcgcc cgtcgcgacc acaaccgcct ccccccacga gcgtcgccag
     9901 cgccagtgct cgtgcaacga tctgcggccg tgccgcgatc atagcgccac gccgccctgg
     9961 ttggccgcta tcgcggcgcg cagggagtcg acgagcgcgt gtttgttcac ccgagccccc
    10021 gccacggaaa cgtcggcggc gccgtaatac acgagcaggt catctccgcg ctcgacgagc
    10081 ccctggggaa agacgacgcg cactcccgtc gggaagttcg cggggtccac ctgcgggatg
    10141 tgttgcatcg cgatctcata gggcgcctgc ggggcgaggg cgggctcatc ggagacgtac
    10201 ttcaccgtct cgggacggtg caggtcgagc acgcaccatc ccatggagta gacattgcct
    10261 tcgtcgatcg cggtcgcgcc gtgaacgggg agtagccatc cgatctccgt acggatcggt
    10321 ggcgccccgg cgccgactcg cgcgctccgc cacgttccgg cgcgcggtcc cacgagcgcg
    10381 atgccgtccg cgggccagga gtcgtcgagg gaacgcaccc gcgcgcacac catgcggggc
    10441 atggggcgat ggaggatgac atagtcgccg tcgatcttct gcgggaagat cacgcagttc
    10501 ttgttgtcgc cggcgatgcg cggcccgtgc cggcggtagc gccccggcgt cagcacatca
    10561 tccgtggtga tcaggcagac gcgcggcccg tcgcgggaat agccggtata ggtgatcgcg
    10621 tagcggcggt cgtcctcgag gtaggtgatg cgcggatcct cgacgcccca ctcctcgtac
    10681 ggcgcctcgc gcgcaaacgc gggatgagcc cagatgtcgt cgatccgctc gccgtccgcc
    10741 gacagggcga gcgccaggca cgaacggcgt gcctgctcct cgaacacccg cacgagcagg
    10801 acgactcggc cgctcgcgcg atcgatggtc gcgcccgggt tgaacgaacc ggtggcgcgc
    10861 atgaagggga tctgggcggg cgtgatcagt ggattcgacg ccagacgctc gagcggcgcg
    10921 atcacgatgg cagcgggccc ggcgtccgct ccagccacgt cgcgtagagg cgcgcgaagt
    10981 gggaagccgg ggtccgatag tattcgtccg cactcacgtc gagcagggca ggaaccgggg
    11041 atgtggggag cgtgccggcg tcgcgtctcg ccttgaacgc gcggaacacg cgcaccgcgt
    11101 gcttctcgct tccgtcggcg cgcacgagtc cgaaggagcg ctcgcgccgg gccgtcgc
//
LOCUS       DBNGZW010000625         2270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1220276, whole genome shotgun sequence.
ACCESSION   DBNGZW010000625 DBNGZW010000000
VERSION     DBNGZW010000625.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1220276"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="AC16JC_14705"
     CDS             complement(<1..591)
                     /locus_tag="AC16JC_14705"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5165998.1"
                     /translation="MSRLPRPVLLFGLVSLFNDFASEMIYPLLPAFVTGVLGGGAVSV
                     AAIDGASEFAASLVKYWAGRLADDQRRRGGLIILGYLVAVLVRPVIALTGAAWQVIGL
                     RVTDRVGKGVRTPPRDALIADVTPPAQLGRAFGFQRGLDHAGAVLGPLVAWWLLTSHE
                     LDLRGVIGASIVPGVVVLVLAVWAVRDGRRRERAEEG"
     gene            complement(584..2197)
                     /locus_tag="AC16JC_14710"
     CDS             complement(584..2197)
                     /locus_tag="AC16JC_14710"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR5165999.1"
                     /translation="MALSAFRELERSYFDLRWHLDPVAATLAGLTTHDDHFGRYSPTV
                     LAPHLAALKSIGAALEESMGDDVQLEIDRTALLNEIRVTIRQFEQQRPQAKNPEFWLT
                     HLFNGLYGLLRRSDRGSAANAASVAARLEDVAAFLDDARAAIVEPVRLFAETALQMCP
                     GGVLLLREIRSAYADSPAGERVGAAAEAAGAALAAFEHDLDRWVEAGSGHFAVGEDEF
                     NFLLHYQHALRDTAPELWRYGLRLKDEVTADLSRRAERLAGNADWPALVGRLRGDHPS
                     AQGLVAAYAREMERAREFVAARGLAPLPAVPLDVIATPEFMRPLLPFAAYDSPGPYSA
                     SPRGQFYVTVPDPALPEAARERVLRDHSTHEIAATALHEGYPGHHLQLVLAQANASET
                     RKNVTTPLTVEGWALYCEELMADEGFYSSEEEKFFQRVHLLWRAMRIVVDVGLHTRGM
                     SYDEAVRLMVDGVHMEQSLAEAEVDRYCSTPAYQLCYGVGRRELLRLRDDYRSAQGGA
                     FTLRGFHDVVLSYGGLPVTLMRWGMGLGE"
BASE COUNT          350 a          844 c          776 g          300 t
ORIGIN      
        1 gccttcctcc gccctctccc gccttcttcc gtctctgacc gcccacacgg cgagcaccag
       61 caccaccacc cccggcacga tgctcgcgcc gatcacgcca cggagatcga gctcgtgcga
      121 cgtcagcagc caccaggcga cgagcgggcc cagcaccgcg cccgcgtggt ccaggccgcg
      181 ctggaatccg aacgcgcgcc cgagctgtgc cggcggggtg acgtcggcga tcagcgcgtc
      241 gcgcggcggc gtgcggacac ccttccccac ccggtcggtc acccgcagac cgattacctg
      301 ccaggcggcg cccgtgaggg cgatcaccgg ccgcaccagg accgcgacga ggtagccgag
      361 gatgatgagg cccccgcgcc gccgctgatc gtcggccagg cgtcccgccc agtacttcac
      421 gagcgaggcg gcgaactccg aggcgccgtc gatggcggcg accgagaccg cgccgccacc
      481 cagcacgccc gtcacgaagg cggggagcag gggatagatc atctcgctgg cgaaatcgtt
      541 gaacagcgag acgagcccga agagcaagac cgggcggggg agcctactca cccaggccca
      601 tgccccagcg catcagcgtg accggcagtc cgccgtagga gaggaccacg tcgtggaagc
      661 cgcgcagcgt gaaggcgccg ccctgcgccg accggtagtc gtcccgcagg cgcagcagct
      721 cgcgccgccc caccccgtag cacagctggt aggccggggt ggagcaatac cggtccacct
      781 cggcttcggc gagggattgc tccatatgca cgccgtccac catcaggcgc accgcttcgt
      841 cgtagctcat cccgcgggtg tgcaggccga cgtcgaccac gatccgcatg gcacgccaca
      901 ggagatggac gcgctggaag aacttctctt cttccgagga atagaacccc tcgtccgcca
      961 tcagctcctc gcagtacagc gcccacccct ccaccgtgag gggggtggtg acgttcttcc
     1021 gcgtctccga ggcgttggcc tgcgcgagga cgagctgcag gtggtggccg ggatagccct
     1081 cgtgcaatgc ggttgccgcg atttcgtggg tcgagtgatc gcgcaggacc cgctcgcgcg
     1141 cggcttccgg cagcgccgga tccgggacgg tcacgtagaa ctggccgcgc gggctcgccg
     1201 agtacggtcc cgggctgtcg taggcggcga aggggaggag gggacgcatg aattcgggcg
     1261 tcgcgatcac gtcgagcggc acggccggca gcggcgccag cccgcgcgcc gccacgaact
     1321 cccgcgcgcg ctccatctcc cgcgcgtagg ccgcgaccag cccctgggcg gatggatggt
     1381 cgccgcgcag ccggcccacc agcgccggcc aatcggcgtt gccggccagc cgctcggcac
     1441 ggcgcgacag gtcggcagtc acctcgtcct tcaaccggag gccgtagcgc cacagctccg
     1501 gcgcggtgtc gcgcagcgcg tgctggtagt gcagcaggaa gttgaactcg tcctcgccta
     1561 ccgcgaagtg tccgctcccc gcctcgaccc aacggtcgag gtcgtgctcg aacgccgcga
     1621 gggccgcccc ggctgcctcc gcggcggcgc ccacccgctc acccgccggg ctatcggcgt
     1681 acgccgagcg gatctcccgc agcagcagga cgcccccggg gcacatctgc agcgccgtct
     1741 cggcgaacaa ccgtaccggc tccacgatcg ccgcccgggc gtcgtccagg aacgccgcta
     1801 cgtcttccaa ccgcgcagcg accgacgccg cgttggccgc ggagccgcgg tccgaccgcc
     1861 gcagcaggcc gtagaggccg ttgaacaggt gcgtcagcca gaactctggg ttcttcgcct
     1921 gcgggcgctg ctgctcgaac tgccggatgg tgacgcggat ctcgttcagc agggcggtgc
     1981 ggtcgatctc gagctggacg tcgtccccca tggactcctc gagcgccgcc ccgatcgatt
     2041 tgagcgccgc gaggtgcggg gccagcacgg tgggactgta gcgaccgaaa tggtcgtcgt
     2101 gcgtcgtgag acccgccagc gtcgccgcga cgggatcgag atgccagcgc agatcgaagt
     2161 acgaccgctc gagctcccgg aacgcgctca gcgccatgcg ccctgccgca gcaggctgtg
     2221 gcattcctcc gcggtcagcg tgtggtcggc ccgcttcgcg gcgtcgaaca
//
LOCUS       DBNGZW010000626         7360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1816470, whole genome shotgun sequence.
ACCESSION   DBNGZW010000626 DBNGZW010000000
VERSION     DBNGZW010000626.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7360
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1816470"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(41..514)
                     /locus_tag="AC16JC_14715"
     CDS             complement(41..514)
                     /locus_tag="AC16JC_14715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166000.1"
                     /translation="MRLVVCSVSLLALACAQDPPSAERNPTRIEAAADQEFRLAIGET
                     AAVTGTDLRITFRAVVNDSRCPSAVRCVSAGNAEIALQVRQGSADTTFLLNTTNGPRT
                     GTRPGFIIELIALTPYPERPESPIPAGVYRAGLTVSILPRLTVIPLRPVRTSSPN"
     gene            complement(649..1140)
                     /locus_tag="AC16JC_14720"
     CDS             complement(649..1140)
                     /locus_tag="AC16JC_14720"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5166001.1"
                     /translation="MTIGESLLPEFDQEMATTRTLLERVPSDRGQWKPHPKSFPLGHL
                     AQLVSWIPGWITTTLQQPSLDLATGGGYSFETTETLVRQFDANVRSARAALAGATDAD
                     FEQPWFLKHGDRVLFTAPRGTVVRNHISHLVHHRGQLTVYLRLNDVPLPPIYGPTADA
                     RPW"
     gene            1405..4314
                     /locus_tag="AC16JC_14725"
     CDS             1405..4314
                     /locus_tag="AC16JC_14725"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5166002.1"
                     /translation="MAIRVHEGTTLGFDLSPDGRSIVFDLLGALWELPSAGGTARPLT
                     DAVRDTAEDLDPSYAPDGRRIVFRADRHGRTGLWLLEPGGAPLQLTQLQNPDGYEGNA
                     AWSTDGKTIAYARLVPPDSGVPRWHSRLARIDLATRAVRDLPVAESVGLNLRDPAFEP
                     GGTRLVVVAGFAPAPAGGRMWLVESVSGRATPLLPATTEALAPAFSPDGRRLAFFAPD
                     SGARMQLWMLQLDSPGAAAVRLTNHDDVTPTRVRWTPDGRRLVYSADGRLWKVSASGG
                     PPVEIPFTVRLSFERPKRSLPPARFPEPGTAQPVRAFMGLALSPDARRVAMLALGKLW
                     VMPVDGTPQAVADVPLSAHHLAWSADGAILAWSAGPWQEQDVFATEIATGSTHRITAL
                     PGREEHPSYSPDGRHLAFLYQPSEDTTILRVVDALARDVSDTTQGSSLPAENGADVSW
                     TPDADGLLVISGGFNPNDSTRGTIIRLSGERRALARMPDSPLFLRWTDSSVVFVRHAR
                     LWRAPFDSNGMRGPAEPIGSEPDMYASSANDGAVLFISDGGLHLRSPDGREQRLGWPL
                     SYTPPVADPLLIRNARIIDGTGRPATAPRDLLVERGRISRIAAAGTLPAGTARVLDAG
                     GRYLIPGLMDLHAHEYRPELLPGFSYFGVTTIRDQGSPIGPLVAYADAIAAGQLDGPR
                     VDYGGFQFYSDWGYDAEDQQGVEPEADPDHVSRAVEMAQVFGSQHIKTRTFRRWDINA
                     RMIAEAHRRGMRATGHCAHLLPLVAAGMDAKEHAGFCSARSDGLIYDDLVQLYRTAGI
                     GVVPTIAYASFAVHLNEHPDLLDADSALAPFLPPRNSFAWMLRLNAAMRQQFAQFAQR
                     ARAATARLARAGVTIGTGTDIWQIPTGVHLELEELVAAGLSPLEAIHAATGGAARIVG
                     AEQDLGTIAAGKLADLVLLDADPVVDIRNTRRIRAVIQAGHLVDRPGIVGRFARR"
     gene            complement(4351..5574)
                     /locus_tag="AC16JC_14730"
     CDS             complement(4351..5574)
                     /locus_tag="AC16JC_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012813843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166003.1"
                     /translation="MSVAARWKGFSTRGPLVVGAAALIACTERPAVTAPPAGSVAPPP
                     AVAAGAPLDRSAQGRYIFRHWTFGDEPFWTDTLHLDQVVETAVSPATALAVGLKVDAE
                     RLPPGFLSGADLTSPATTVELLRRDAVVGVEAVVTADRRIQSLGITCALCHSTVDNSV
                     APGVGRRLDGWPNRDLNVGAIVALSPALPPAAKAVFNSWGPGKYDAEFSRDGINSPVV
                     IPPAFGLRGVALETYTGEGPVSYWNAYVAVTQMHGHGSFADPALGIDIVQIPDRVRPV
                     LPVLRAYQLSLSAPPPPSGSVDAAAAERGRDLFNTTARCATCHIPTMAFSDVNLGVLH
                     DPAETGMDPVRAGRLKNHQYRTTPLRALWQHPPYFHDGSAATLADVVGHYDRVFGLGL
                     SAQQRMDLVEYLKSL"
     gene            complement(5844..>7360)
                     /locus_tag="AC16JC_14735"
     CDS             complement(5844..>7360)
                     /locus_tag="AC16JC_14735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166004.1"
                     /translation="KIRVSTQTTGGVTPDVTGAETFCTIVSLAESPMRPGLLYAGTDD
                     GNVWLTRNDGGTWENLTGRFPGVPAGTYVSRIEPSHFDTATFYVTFDNHRNNDFAPYV
                     YVTTDFGRTFRSIANDLPTGGPNYVHVIREDPANRDLLFLGSDVGLYVSRDRGANWQR
                     FMTGFPTVPVHDLRIHPREHELIAATHGRGVWIVEISPLEQLTSEVLAAGAWLFTPKT
                     AYQYGEPTRANFSAGQKWFRAASPPYGAEIAYRLTTGERRTQTKIVITDVRGDTVRTV
                     NGPGGPGLHRATWNFAGPLPQRRPLSPSQKRDSVLLTARINVVFDSLAKAGMSPQMLD
                     PIKTALLEGDVQGLAQRFGFGGGGGGGGGGGGAAAAGGRFVERPGETTPRAPGAAGAA
                     AGAQQQEGGEAAADQPDPTFLGTLGQLIRLPSQRGGGGGGGGFGALGFVAQTFGRAAG
                     GGGGFGGFGAANTVQSGNYLVSITVDGKTLSRVLRVERVAASGATVATFDDDYR"
BASE COUNT         1067 a         2535 c         2622 g         1136 t
ORIGIN      
        1 ggcggcgcgc gtcatccccg cgcgccgcca tgtcacgcga tcaattcggc gacgacgtcc
       61 gcaccggacg cagcgggatc acggtgagtc gcgggagaat ggagacggta agccccgccc
      121 ggtacacccc ggcgggaatc ggcgactccg gccgttccgg gtacggcgtg agggcgatga
      181 gctcgatgat gaagcccggc cgggtccccg tccgcgggcc gttggtcgtg ttcaggagga
      241 aggtcgtgtc tgccgagccc tgtcggacct gaagcgcgat ctccgcattc ccggcgctga
      301 cgcaccggac ggcgctgggg cagcgggagt cgttcaccac cgctcgaaac gtgatccgca
      361 ggtccgttcc ggtgaccgcg gccgtctcgc cgatcgcgag gcggaactcc tggtcggcgg
      421 cggcttcgat cctggtggga ttccgctcgg ccgacggggg atcctgggcg caggcgagcg
      481 cgagcagcga aaccgaacaa acgacgagtc gcatgggcgt tctccgctgt gggtgagcga
      541 ccggactgct acctggacat accctcacgg gtcagccggt ggtcacggcg acagcgacgc
      601 atcccggcca ctccccgaac cggaagagca ccaggtcccg cccgcggact accacggccg
      661 ggcgtcggcg gtgggcccat agatcggcgg cagcggcacg tcgttcaggc gcagatacac
      721 ggtcagctgg ccgcgatgat gcaccaggtg actgatatga ttgcgcacca ccgtgccccg
      781 cggggcggtg aacagcaccc ggtcgccgtg cttcaggaac cagggttgtt cgaaatccgc
      841 gtccgtggcg cccgcgagcg cggcacgggc actccggacg ttggcatcga actgtcgcac
      901 gagggtttcc gtcgtctcga agctgtagcc acccccggtt gcgaggtcga gagacggctg
      961 ttggagggtc gtggtaatcc accccgggat ccacgacacc agctgcgcca gatgccccag
     1021 cgggaacgac ttgggatgcg gtttccactg tccccggtcg ctcggcacgc gctctagcag
     1081 ggtccgcgtg gtcgccattt cctggtcgaa ctcgggaagg agagactcgc cgatggtcat
     1141 agggcttctg cgctcgtgtc gatggcagca aaaaagtata acgcgcgtgt cgcgaaaccc
     1201 gcagcaggct gcatgcgtcc tacgggagcc gcacggctgg acacggatga tcggcagcat
     1261 caaggttcat gtccacttcc tggcacagga gactcggtga ccctggtccg atacggtcgc
     1321 atcacgtcgg cgatcgctgt ggctgccatc ttctgctcga ggtccgcgcc ggggcagagg
     1381 gcttcgcgcg gcgctatgga aaccgtggcc attcgcgtac acgagggaac caccctcggc
     1441 ttcgatctgt cgcccgacgg gcgctcgatc gtgttcgacc tcctcggggc attgtgggag
     1501 ttgcccagtg cggggggaac ggctcggccc ctgaccgacg ccgtacgcga cacggccgag
     1561 gatctggacc cgtcctacgc ccccgacggt cggcgcatcg tgttccgcgc cgatcggcac
     1621 ggccgcaccg gattgtggct gctcgaaccg ggcggcgctc cgctgcaact cacccagctc
     1681 cagaacccgg acggatacga gggcaacgcg gcgtggtcga cggacgggaa gaccatcgcc
     1741 tacgcccgcc tggtgccccc ggacagcggc gtgccgcgct ggcacagccg gctggcccgg
     1801 atcgatctcg ccacccgggc ggtgcgcgac ctgccggtcg cggagtccgt gggactcaat
     1861 ctgcgcgatc cggcattcga accgggggga acacgtctgg tggtcgtggc gggcttcgcg
     1921 cccgctcctg ctggcggccg gatgtggctg gtcgagagtg tcagtgggcg cgcgaccccg
     1981 ctcctccccg cgaccacgga ggcactcgct ccggccttct cacccgatgg ccggcgtctc
     2041 gccttcttcg cgccggactc aggtgcgcgg atgcagctct ggatgctgca gctggattcg
     2101 cccggcgccg cggcggtgcg cctcaccaac catgacgacg tgacgccgac gcgggttcgc
     2161 tggaccccgg acggtcgccg gttggtgtac agcgccgacg gccgcctgtg gaaggtctcg
     2221 gcgtcaggcg gaccgccggt agagattccg tttaccgtca ggctgtcctt cgagcgtccg
     2281 aagcgctcgc ttcctcccgc ccgcttcccc gagcccggca cggctcagcc cgtccgcgcg
     2341 ttcatgggtc tcgcactctc acccgacgcg cggcgcgtgg cgatgctggc actcggtaag
     2401 ctgtgggtga tgccggtgga cgggacgccg caggctgtcg cggacgtgcc gttgagcgcg
     2461 catcacctcg cctggtcggc cgacggcgcc atcctcgcgt ggtcggcggg gccgtggcag
     2521 gagcaggacg tgttcgcgac cgagatcgca accggctcga ctcatcggat cacggccctg
     2581 ccgggtcggg aggagcaccc gagctactcc cccgacgggc ggcatctcgc atttctgtac
     2641 cagccgtcgg aggacaccac gatcctccga gtggtcgacg cgctggcgcg tgacgtgtcg
     2701 gatacgactc agggctcgtc ccttcctgcc gagaacggcg cggatgtgtc gtggacgccg
     2761 gacgccgacg ggctgctggt catctcgggc ggattcaacc ccaatgattc gaccaggggg
     2821 acgatcatcc gcctttcggg tgagcgacgg gccctcgctc gcatgcccga ctcacccctc
     2881 tttctgcggt ggaccgacag ctcggtcgtg ttcgtccgcc acgcccggct gtggcgggcg
     2941 ccgttcgatt ccaacgggat gcgcgggccg gcggagccga tcggatcgga gccggacatg
     3001 tacgcctcct cggcaaatga cggggccgtc ctcttcatct ccgacggcgg gctccatctg
     3061 cgctcgcccg atggacggga gcagcgcctc ggctggccgt tgtcctatac gccgcccgta
     3121 gccgacccgc tgctgatccg gaatgcccgg atcatcgatg gaaccggacg gccggcgaca
     3181 gcgccgcggg atctgctggt ggagcgcggg aggatcagcc ggatcgccgc cgcggggacg
     3241 cttccggcag ggacggcgcg ggtgttggac gcgggcggcc ggtacctcat tcccggcctc
     3301 atggatctgc acgcgcacga gtaccgtccg gagctgttgc cgggattctc ctatttcggt
     3361 gtgacgacga tccgcgacca gggatcgccg atcggtcccc tggtcgccta cgccgatgcc
     3421 atcgccgcgg ggcagctgga cgggccacgg gtggactacg gcggcttcca gttctacagc
     3481 gactggggct acgacgcgga ggaccagcaa ggtgtggagc cggaagcgga ccccgaccac
     3541 gtgtcgcgcg cggtcgagat ggcacaggtt ttcggctcgc agcacatcaa gacgcggacc
     3601 ttccggcgct gggacatcaa cgcgcggatg atcgcggagg cacaccgccg cgggatgcgc
     3661 gcgacgggcc actgcgctca tctgctgccg ctcgtcgcgg ccggtatgga tgccaaggag
     3721 cacgccggat tctgctcggc gcgcagcgac gggctcatct acgacgatct ggtgcagctg
     3781 taccgcacgg cgggcatcgg cgtggtgccg acgattgcgt atgcgagctt cgccgtccac
     3841 ctgaacgagc atccggatct gctcgacgcc gattcagccc tcgccccgtt tctcccgccg
     3901 cgcaacagct tcgcctggat gctgcggctg aacgcagcca tgcgccagca attcgcccag
     3961 ttcgcgcagc gggcgcgggc tgcgaccgcc aggctggcac gcgcgggtgt gacgatcggc
     4021 acgggaacgg acatctggca gatcccgacg ggcgtgcacc tcgagctgga agagctggtc
     4081 gccgcgggac tgtcgccgct cgaggcgatt cacgccgcga ccggcggcgc tgcgcggatc
     4141 gtcggcgccg aacaggatct cggcacgatc gcggcgggga agctggccga cctggtcctc
     4201 ctcgatgccg acccggtggt ggacatccgc aacacccgcc gcatccgggc cgtcatccag
     4261 gcgggccacc tcgttgaccg tccggggatc gtcggccggt tcgcgcgccg ttgagcggag
     4321 gtcgaggcgg ccactcccgc gagacttccg ctacagcgat ttcaggtatt cgacgagatc
     4381 catcctctgc tgggccgaga ggccgagtcc gaagacgcgg tcgtagtgcc cgaccacgtc
     4441 cgccagcgtg gccgcgctgc cgtcgtggaa gtacggcgga tgctgccaca gggcgcggag
     4501 cggcgtggtg cggtactggt ggttcttgag gcggccggct cgcaccgggt ccatacccgt
     4561 ctcggccggg tcgtgcagga cgccgagatt cacgtcgctg aacgccatcg tggggatgtg
     4621 gcaggtagcg cagcgggcgg tcgtgttgaa caggtccctg ccgcgttccg ctgccgctgc
     4681 gtcgacgctg cccgaaggcg gcgggggtgc gctcaagctg agctggtagg cccgcagcac
     4741 cggcaggacg ggcctcacgc gatcgggaat ctgtacgatg tcgatgccca gggcggggtc
     4801 ggcgaacgag ccgtggccgt gcatctgcgt caccgcgacg taggcgttcc agtaggagac
     4861 cggtccttct cccgtgtagg tctcgagcgc cacgccgcgc agaccgaagg ccggtgggat
     4921 caccaccgga ctgttgatgc cgtcacggct gaactccgca tcgtacttcc ccggacccca
     4981 ggagttgaat accgctttgg cggcgggcgg cagggcgggg gagagcgcca cgatcgcccc
     5041 cacgttgaga tcgcggttgg gccagccgtc cagccggcgc ccgacacccg gtgccacgga
     5101 gttatccacg gtcgagtggc acagggcgca cgtgatgccg aggctctgga tgcgccggtc
     5161 cgcggtaacg accgcttcga cgcccacgac cgcatcccgg cgcagcagct cgaccgtggt
     5221 ggccggactg gtgaggtccg cgccgctgag gaagcccggc ggcagacgct cggcgtcgac
     5281 cttgaggccc acggccagcg ccgtggccgg ggagacggcg gtttcgacga cctggtcgag
     5341 gtggagcgta tcggtccaga acggctcgtc gccgaaggtc cagtggcgga agatgtaccg
     5401 accctgggcg gagcggtcga gcggtgcgcc cgcggcgacc gccggcggcg gggccacgga
     5461 gcccgcgggg ggagccgtca ccgcgggacg ttcggtgcag gcgatcagcg cggccgcacc
     5521 gacgaccagc ggcccgcgcg tgctgaaacc cttccaccgt gctgcaacgg acataacggc
     5581 ctcctgggga ggcggggcgc ggacacggtc cctgccggga acgaacccgc gctccgccca
     5641 accccgcatg ggtgtgcggt tcgttctata acatgtgtac gctctgttta gagggatcgg
     5701 ttctgtcggt cactcccaca cggcagaagg cccagcggcg cctgcgaggc cgctggggct
     5761 gaccccggcg gcaggcgctg ctggacctcc ttcagacgac ggcgcaggtg gtggggggcg
     5821 ctaaccgcct ggtgcgcggc tcctcatcgg tagtcgtcgt cgaatgtggc caccgtcgcg
     5881 ccgctcgcgg cgacgcgctc gacccgcagc acgcggctca gcgtcttccc gtcgacggtg
     5941 atgctgacca ggtagtttcc gctctgcacc gtgttcgccg cgccgaagcc gccgaacccc
     6001 ccgccgccgc cggccgcacg gccgaaggtc tgggccacga accccaaggc gccgaacccg
     6061 ccgcccccgc cgcccccgcc acgctggctc ggcaggcgga taagctggcc cagtgtgccg
     6121 aggaacgtcg gatcgggctg atccgccgcg gcctcgcccc cttcttgctg ctgcgcaccg
     6181 gccgccgcgc cggcggcacc gggcgcgcgt ggcgtcgtct caccgggccg ctcgacgaac
     6241 cggccgccgg cggcggcagc gccgcccccc ccgccgccgc ccccgccccc gccaccgaag
     6301 ccgaaccgct gcgccagccc ctgcacatcg ccctccagca gggcggtctt gatggggtcg
     6361 agcatctgcg gactcattcc ggccttggcg agcgagtcga acaccacgtt gatgcgggcc
     6421 gtgagcagca cgctgtcccg cttttgcgac ggcgagagtg gtcgccgttg tggcaacgga
     6481 cccgcgaagt tccaggtcgc gcggtgcagc cccgggccgc cgggaccgtt caccgtgcgc
     6541 accgtgtccc cccgcacgtc ggtgatgacg atcttggtct gggtgcggcg ctcacccgtg
     6601 gtcagccggt aggcgatctc cgcgccgtac ggcgggctgg ctgcgcggaa ccacttctgg
     6661 ccggccgaga agttggcgcg ggtcggctct ccgtactggt aggccgtctt cggggtgaac
     6721 agccacgcgc ccgccgccag cacctcggac gtcaactgct cgagcggcga gatctccacg
     6781 atccagacgc cacggccgtg cgtcgcggcg atgagctcgt gctcgcgcgg gtggatgcgg
     6841 aggtcgtgca ccggcacggt ggggaacccg gtcatgaagc gttgccaatt cgccccgcgg
     6901 tcgcgtgaga cgtacaggcc gacgtcggag ccgaggaaga gcaggtcgcg gttggccggg
     6961 tcctcacgga tcacgtgcac atagttcggc ccgcccgtcg gcaggtcgtt ggcgatggag
     7021 cggaacgtcc tgccgaaatc cgtcgtgacg tacacgtacg gcgcgaagtc gttgttgcgg
     7081 tgattgtcga acgtcacgta gaaggtggcg gtgtcgaaat gcgacggctc gatgcggctg
     7141 acgtacgtcc ccgcggggac gcccgggaag cggccggtca ggttttccca ggtgccgccg
     7201 tcgttccggg tcagccagac gttgccgtcg tcggtgccgg cgtacagcag gccgggccgc
     7261 atcggcgact cggcgagcga tacgatggtg cagaaggtct ccgcccccgt cacatcgggc
     7321 gtgaccccac ccgtcgtctg ggtgctcacc cggatcttca
//
LOCUS       DBNGZW010000627         2245 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_58447, whole genome shotgun sequence.
ACCESSION   DBNGZW010000627 DBNGZW010000000
VERSION     DBNGZW010000627.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_58447"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="AC16JC_14740"
     CDS             complement(<1..620)
                     /locus_tag="AC16JC_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019668879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR5166005.1"
                     /translation="MAGSDWLIILLYFGLLLGLTWRVLRKSKDTADDYFLASRNLGWF
                     IVGASIFASNIGSEHLVGLAGAGATSGVALAHYELHAWCLLVLGWVFVPFYTRSMVYT
                     MPEFLERRFSPGARWVLSIISLVAYVLTKFAVGIFAGGVVFATLLPDVQLQLGGATFN
                     SFWVGSVAVVVLTGLYTVVGGMRGVAYTEAAQTIILILGSVLITVFG"
     gene            complement(702..>2245)
                     /locus_tag="AC16JC_14745"
     CDS             complement(702..>2245)
                     /locus_tag="AC16JC_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014265828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HIR5166006.1"
                     /translation="SAYMDLNDVPASGNRWLLTDVLRREWGFRGFVTSDAFAVASLQA
                     HGFASDPADATSKAITAGAGMDMASQTYRNHLAQLVRSANVSPAQIDAAVLPILEVKF
                     RIGLFEHPYANESAGATEVSAEGRSLARRLGARSMVLLKNDDHALPLSNTIKNVAVIG
                     SLADSPGDITGGPTPAGIFGQGPDAPAVTVLAALKTRLDPEAHVGYVPGPAMSKVFPS
                     MFDAFMGHKPVPPPTPAEVADWLAQATAAAAQADIVIAVIGEPAFMSGEAASRGTLGL
                     PGIQEQMVEAAAASGKPLIVVLENGRPLDINWVAEHAAAILEAWFPGVEGGNAIVDVL
                     FGDVNPGGKLPVSWPRSAGQEPLYYNHNLTQTPESDPQFTSRYWDMPSTPLYPFGYGL
                     SYTTFRFANLHLSKTRMKAGDSVAVAVDVTNTGSAPGDAVAQLYIHQRSGSASRPVRQ
                     LEGFRRVTLQPGETRTLRFSLGRNELQFWSPQTKRWVVEPSRFDVWVGADATADLHAE
                     LTIGG"
BASE COUNT          397 a          734 c          766 g          348 t
ORIGIN      
        1 ccgaacacgg tgatcagcac cgagccgagg atcaggatga tggtctgcgc cgcctcggtg
       61 taggcgaccc cgcgcatccc gccaaccacg gtgtagagcc ccgtcagcac gacgacggcg
      121 accgagccga cccagaagct gttgaacgtc gctccgccga gctgcagctg cacgtcgggg
      181 aggagcgtgg cgaacacgac gccgcctgcg aagatgccga ccgcgaactt ggtgagcacg
      241 tacgcaacga gcgaaatgat cgagagtacc cagcgcgcgc ccggcgagaa gcgccgctcc
      301 agaaactcgg gcatcgtgta caccatcgag cgcgtgtaga acgggacgaa aacccatccc
      361 agcacgagca gacaccaggc gtgcagctcg taatgggcga gcgccacgcc gctggtggcg
      421 ccggcgccgg cgagccccac gaggtgctcg gagccgatgt tggaggcgaa gatcgaggcg
      481 ccgacgatga accagccgag gttccggctg gccagaaagt aatcgtccgc ggtgtccttg
      541 ctcttgcgga gcacccgcca ggtgagcccc agcagcaggc cgaaatacaa caggatgatg
      601 agccagtccg agccagccac acccgctccg ccgcgagagt cggagggacc ctacacggcg
      661 cggcgccggt gtcaagaaag gccggtggct cgtcgccgcc ttcagccccc tatcgtgagc
      721 tccgcgtgca gatccgccgt ggcatctgct ccgacccaga catcgaagcg gctgggttcc
      781 accacccagc gcttcgtctg ggggctccag aactggagct cgttccggcc cagggagaaa
      841 cggagcgtcc tggtctcgcc gggttgcagg gtcactcggc gaaacccttc gagctggcgc
      901 accgggcgtg acgccgagcc ggagcgctga tggatgtaga gctgcgccac cgcgtcaccc
      961 ggcgcggaac cggtattggt cacgtccaca gccactgcga cgctgtcccc ggctttcatc
     1021 cgggtcttcg agaggtgcag gttcgcgaac cggaaggtgg tgtagctgag gccatagccg
     1081 aacggataca ggggtgtgct gggcatgtcc cagtagcgtg acgtgaactg gggatcgctt
     1141 tccggcgtct gtgtgagatt gtggttgtag tagagcggtt cctgccctgc cgagcggggc
     1201 cagctgacgg gcagtttccc tcccggattc acgtcgccga acagcacgtc gacgatcgcg
     1261 ttgccgcctt ccacgcccgg aaaccacgcc tcgagaatgg cggcggcgtg ttcggcgacc
     1321 caattgatgt cgagcggccg gccgttctcg agcacgacga tgagcggctt gcccgacgcg
     1381 gccgcggcct cgaccatctg ctcctggatg cccggcaaac cgagtgtgcc acgcgaggcc
     1441 gcttcgccgc tcatgaaggc gggctcgccg atgacggcga tgacgatgtc cgcctgtgcg
     1501 gcggccgcgg tcgcctgcgc cagccaatcc gcgacctccg ccggggtcgg tggcggcacg
     1561 ggcttatggc ccatgaacgc gtcgaacatc gacggaaaca ctttcgacat cgcgggtccc
     1621 ggcacatacc cgacgtgagc ctcgggatct agccgagtct tcagggcggc cagaacggtg
     1681 acggccgggg cgtcggggcc ttggccgaag atgcccgcgg gtgtgggccc tccggtgatg
     1741 tccccggggg agtcggccag ggacccgatg accgccacgt tcttgatcgt gttcgagagc
     1801 ggcaaggcat gatcgtcgtt cttgagcagc accatggatc gggcgccgag tcgccgggcc
     1861 agggaccgac cttccgcgct gacttccgtt gcaccggcgg actcgttcgc gtacggatgc
     1921 tcgaagagac cgatccggaa cttgacctcg aggatcggca ggaccgcggc gtcgatctgt
     1981 gccggcgaca cattcgcgct tctaaccagc tgcgccagat ggttccgata cgtctggctg
     2041 gccatatcca tcccggcccc tgcggtgatc gctttggagg tggcatcggc cggatcggat
     2101 gcgaacccgt gggcttgcag gctcgcgacg gcgaacgcgt cggacgtgac gaagccacgg
     2161 aatccccatt cccgtcgcag cacgtcggtc aggagccagc gattgccgct ggcaggcacg
     2221 tcgttcaggt ccatgtaggc agaca
//
LOCUS       DBNGZW010000628         2532 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_400562, whole genome shotgun sequence.
ACCESSION   DBNGZW010000628 DBNGZW010000000
VERSION     DBNGZW010000628.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2532)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2532)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2532
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_400562"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..742
                     /locus_tag="AC16JC_14750"
     CDS             158..742
                     /locus_tag="AC16JC_14750"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR5166007.1"
                     /translation="MKQADLTRQRLVRAALELFTSQGYHPSTIPLIARRAGIAEGTIY
                     RHFPGKQQLLNELYRGAARWATKLVREADRAGASPRDKLARLGTLLVDGAVREPAVVR
                     LFFFQDYSALLDEPSRTTAREFRGALESLVAGGKADGTVRAGGVDVLAGAWLGVVQVA
                     LERILARDWGMSSAGAQLCRDAAWDAIRARKEID"
     gene            747..2066
                     /locus_tag="AC16JC_14755"
     CDS             747..2066
                     /locus_tag="AC16JC_14755"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR5166008.1"
                     /translation="MPSRFAWILYAPLLAGGAPLGAQQLVPVERARLAAAVWAEARYN
                     APALDESRADWDSALTSMLSAAPERQSDLAFFRRLRRFIALLHDGRAAVVPPGAVGSR
                     WARPPLVLRGIERRPFLVDYAENDEMRVARPERNAEILAVQGISAERWIRDSVLPEIA
                     AATDAARWELAVSRMLEGEKGTAIHLQLRLPGGPERGASVTRSLSLTEDWPLAPPPLQ
                     VDTFTDGVVWVRISSFADPDVARRFDRAFPDFTRVRGLVLDLREHEGASGGRETGYRI
                     LGRLVAKPLITTRWRTPQYRPAYRGGDMPDSAWRWLEAPPDTVPARTDLPVFTGPVAV
                     LTSARTGGSAEDLLVAFREAGRGPIVGQTTAGSAGQVGEFRLYRSWRLQLTVTRDAFP
                     DGAEIQGRGIAPEVPVTQKVSDFLAGRDATLERARGYIAEHQVNPSR"
     gene            complement(2105..>2532)
                     /locus_tag="AC16JC_14760"
     CDS             complement(2105..>2532)
                     /locus_tag="AC16JC_14760"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR5166009.1"
                     /translation="LNILKDGKFYGWPQCYLPGQPNPEYPSASCSNVEPPAITFQAHS
                     APLGITFYTAAGFPGYAGDAFLTYHGSWNRRIPTGAKVVHVKVQNGKPVSIEDFVTGW
                     QESDGSRWGRPVAVLAMPDGSLLISDDTGGRIWRVTYGK"
BASE COUNT          351 a          902 c          898 g          381 t
ORIGIN      
        1 cagccctgaa ggcccgcgcc gagaaccagc atgcgcatgg ggctcgaaga tctcccgacg
       61 gatgtgaaag gagaatgaag cggcccgaaa gctagccggg gcgcagaagc gccggaagcg
      121 cgcggaagtc tcggcggttc attagattcg ctcgcctgtg aagcaggccg acctcacccg
      181 gcagcgcctc gtgcgcgcgg cgctggagtt gttcacgtcg cagggctacc acccgagcac
      241 cattccgctg atcgcccgcc gggcggggat cgccgaaggc accatctacc ggcacttccc
      301 cggaaagcag caactcctga acgagctgta ccgcggcgcg gcgcgctggg cgacgaaact
      361 ggtgcgggaa gcggaccgcg ccggcgcatc gccccgcgac aagctcgccc gcctgggaac
      421 cctgctcgtg gacggcgcgg tgcgcgagcc ggcggtggtc cggctgttct tcttccagga
      481 ctacagcgcg ctgctcgacg agccgtcgcg cacgaccgcc cgcgagtttc gcggcgccct
      541 cgagagtctg gtcgccggcg gcaaggccga cggtacggtg cgcgcgggcg gtgtcgacgt
      601 gctggcgggc gcgtggctcg gcgtggtgca ggtggcgctg gagcggattc tcgcccggga
      661 ctgggggatg tcgagcgccg gcgcgcaact gtgccgcgac gccgcgtggg acgcgattcg
      721 ggcgaggaag gaaatagatt aggcgtatgc cgtcgcggtt cgcctggatc ctgtatgcgc
      781 ctctcctcgc ggggggggcg ccgctcggcg cccagcagct cgttcccgtc gagcgagccc
      841 ggctcgccgc cgcggtctgg gcggaggcgc gctacaacgc ccccgccctg gacgagtcgc
      901 gggccgactg ggactccgcc ctgacatcca tgctgagcgc cgcgccggag cgccagagcg
      961 atctcgcgtt cttccgccgg ttgcgccgtt tcatcgccct gctgcacgac ggacgagccg
     1021 ccgtcgtccc gcccggtgcc gtcgggtccc gctgggcgcg gccgccgctc gtgttgcgcg
     1081 gcatcgagcg ccgcccgttt ctcgtggatt acgccgaaaa cgacgagatg cgcgtggcgc
     1141 ggccggagcg caacgcggag atcctcgcgg tgcagggcat ctcggcggag cggtggatca
     1201 gggattcggt gctgccggag atcgccgcgg ccaccgatgc ggcgcgctgg gagctcgccg
     1261 tgtcgcggat gctggaaggg gagaagggca cggccatcca cctccagctc cgcctgccgg
     1321 ggggaccgga gcgcggcgcc agcgtcaccc gcagcctgtc gctcaccgag gactggccgc
     1381 tcgcgccgcc cccgctccag gtcgatacgt tcaccgacgg cgtcgtgtgg gttcgcatct
     1441 cgtcgttcgc cgatcccgac gtcgcccgcc gcttcgatcg ggcgtttccc gatttcaccc
     1501 gcgtccgcgg cctggtcctg gacctgcgcg agcacgaggg cgcgagcggc ggccgggaga
     1561 ccggctatcg gatcctgggc cgcctcgttg cgaagccgct catcacgacc cgctggcgca
     1621 ccccccaata ccggcccgcc taccgtggcg gggacatgcc cgattccgcg tggcgttggc
     1681 tcgaggcgcc ccccgatacg gtacccgccc gaaccgatct gccggtgttc acgggccccg
     1741 tcgccgtgct cacctcagcg cgcaccggcg ggtcggcgga ggatctattg gtggcgttcc
     1801 gcgaagcggg gcgtggcccg atcgtgggtc agaccacagc cgggagtgcg ggacaggtcg
     1861 gggagttccg cctctacagg agctggcgct tgcagctcac cgtcacgcgt gacgcgtttc
     1921 cggacggcgc cgaaatccag ggccgcggca tcgcccccga ggtacccgtc acgcagaagg
     1981 tctcggactt cctcgcggga cgggacgcga cgctcgagcg ggcgcgcggc tacatcgctg
     2041 agcaccaggt caatccatcc cgctagccgt aaccctcctc caccgtcctc caccgtcctc
     2101 cccctcactt cccgtacgtc acccgccaga tccggccgcc ggtatcgtcg gagatgagca
     2161 gcgacccgtc cggcatcgcc agcacggcca ccggccgccc ccagcgggac ccgtcgctct
     2221 cctgccaccc cgtgacgaag tcctcgatgg agaccggttt gccgttctgt actttcacgt
     2281 gcacgacctt cgcgccggtg gggatgcggc ggttccacga gccgtggtag gtcaggaacg
     2341 cgtcgcccgc gtagccgggg aaaccggcgg ccgtgtagaa cgtgattccc agcggcgccg
     2401 agtgcgcctg gaaggtgatg gccggcggct cgacgttgct gcagctcgcg ctgggatatt
     2461 ccgggttggg ctgccccggc aggtagcact gcggccaccc atagaacttg ccatccttga
     2521 ggatgttcag gt
//
LOCUS       DBNGZW010000629         6559 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1285823, whole genome shotgun sequence.
ACCESSION   DBNGZW010000629 DBNGZW010000000
VERSION     DBNGZW010000629.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6559)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6559)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6559
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1285823"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1417
                     /locus_tag="AC16JC_14765"
     CDS             <1..1417
                     /locus_tag="AC16JC_14765"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR5166010.1"
                     /translation="SHGEYPALLETNLSSNLLNDNGIPAAANVRNTKDITDYKLISFF
                     GRFNYNISDRYLLAMSLRRDGSSRFGPGNQWGTFPSVAVAWRISQEPFMRGIGSLSDL
                     KLRASWARTGNQAFGDYLQYPTYTYSDGQTQVQFGNQFVTTIRPSAVDEFIHWEKTTA
                     YNAGLDFGFSNQRYSGAIDVYTKNTSDLIFNVPVAAGTNLGNFVTTNIGSMRNRGLEL
                     NLSARVMEGRTSGLGWTASITASHNTNELLTINPSKSVSKILTGNISGGVGNLVEVLE
                     PGVPVNSFFVYEQKYANGKPVYDANPLNMYVDQNGDNVINDSDRRPFHDPAPKWIFGH
                     SSYFTFGSHLDASFTVRAYIGNYVYNNVASANGAYQNLTGSGMPSNLHASVLETGFVV
                     PQYYSDFFVEDASFLRMDNITVGYSFDYQGRPFRLYGTVQSAFTLTGYNGVDPTAGLN
                     GIDNNIYPRARTFTAGLSVRF"
     gene            1452..3032
                     /locus_tag="AC16JC_14770"
     CDS             1452..3032
                     /locus_tag="AC16JC_14770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015332668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HIR5166011.1"
                     /translation="MSLAVAFLCVAGIGCTDPTVAPKSTVTSANVFNDPNSYRAFLAK
                     IYGGLIVTGQRGPDGNPDIGGIDEGFGEYLRLYWYLQEMPTEEAVIGWNDPGLPDLNR
                     GTWGADNSMVNAMYYRVFYQIMLVNEFLRQSTDAKLDSRGQNDPALRTNVHFYRAEAR
                     FLRALSYWHGIDLFGNIPLVTETDPLGSTPPKQATRQQLYDYVVGELNAIIGDLPPKG
                     PETYGRATAAAAHMLLAKLYLNAGVYTGTPNYAGALTEAQAVISAGYTLDTSFVHNFQ
                     ADNNTSPELVFVAPQDGNSTQTWGGMTFLIHAGCGGSMSASNYGIDYCWGGYRMKQQA
                     YRLFAAGDKRASFFWTAGQTDSVVAIGDFQYGIAAPKFTNKTSTGSGGAQLGMVDTDF
                     PIFRLADAYLIYAEANLRGGGGSATDALNYFNALRFRAFGDSSAVLTAPQLTLDTLLA
                     ERGRELLFEAQRRTDLVRFGLFESGTYIWAWKGGVAGGAALAAGRNLYPLPANELIAN
                     PNLTQNPGYGAWEGRWST"
     gene            3029..4624
                     /locus_tag="AC16JC_14775"
     CDS             3029..4624
                     /locus_tag="AC16JC_14775"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012356.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004556 - alpha-amylase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HIR5166012.1"
                     /translation="MNPQLRRFDWRGWGFALAFVALACGESGPPITGNPPPDRPSLAP
                     TYRPSGHMASGDVFVHLFEWKWTDIATECETVLGPAGFAAVQVSPPQEHSLEPPTYPW
                     SERYQPVSYSIARSRSGSGAEFQAMVDRCAAVGVGIYVDAVINHMTNYPSPGVGSNGT
                     AYSKYDYPGLYTPADFHPACSVNNYQDAANVQDCELLSLPDLNTGKRSVRQTIAGYLV
                     MLARAGVAGFRIDAAKHIQQVELDSILRIVDSTMTAEGRPIPYYFLEVIGGAGEALAP
                     RDYFGEAYGSGGAADITEFTFTGVGDKFRELAGQRIAQLNPNGPPGSQFSEVAWGLMP
                     SDKAVVFLQNHDTQHQCGLSYRDGDVFRLANVWMLAQPYGYPSILSSFAFACPAGNAM
                     GPPSDGSGNTNDVTCASSLETATTGQWVCEHRDPSIRSMVRFRRVVAGSDVNHWWDDG
                     ANAIAFSRGAKGFVAISREPAALDTTIATGLAPGTYCDVITGGMASGVCAGTAVVVDS
                     GHAVALHLGPNSAVAIDIETRLP"
     gene            4621..>6559
                     /locus_tag="AC16JC_14780"
     CDS             4621..>6559
                     /locus_tag="AC16JC_14780"
                     /inference="COORDINATES: protein motif:HMM:NF012356.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR5166013.1"
                     /translation="MTAPIALLQVTLHLTAVPATTPATATMYVAGTFNGWNPGAYGYT
                     LSAQSGGGYAITLPATVRGRIEFKFTLGSWETVELDSAGGDVSNRSFTVPDSGPAVYT
                     GAVMRWRDGTPRPPPAHSTTASVTVLDTAFVIPQLGRPRRVWVYLPPGYAESRARYPV
                     LYMHDGQNVFDAATSFAGEWGVDESLDSLAAGGDPGVIVVAVDHGGPRRMDEYSPWRN
                     PTRGGGEGDAYVDFLVQTLKPWIDRHYRTRADRGHTGVAGSSMGGLISLYAALKYPNV
                     FGRVGVFSPAFWFAPSIYAYARRAHPLRDTRIFIVMGGEEGDTPAVYVRDAQRMADTL
                     RAAGFRAVQSVVRPDGAHSEWFWRREFPAAYQWLFGDTAPRPSRSPTRRSPPAAWTRG
                     AVCYEIFVRSFYDSDGDGIGDLNGLTKKLDYVRGLGAGCIWLMPVAASPSYHGYDVSD
                     YYKVEPAYGTTDDFKRFVTEAHRRGIAVLVDMVLNHTSSEISWFQAALRDTASSYRHW
                     YRFSPVQGGKGPWGAEAWHRSTVRDEYYYGVFWSGMPDLDYHTPAVREEAKKIATYWL
                     REMGVDGFRLDAVPYLVEDGACMTGCPGTHAFLHEYAAHVDSVKPGAYTVGEAWGNIA
                     TVLPYYPDQLTSYFGFELADSL"
BASE COUNT         1141 a         2240 c         2111 g         1067 t
ORIGIN      
        1 gtcgcatggc gagtaccccg cgctcctgga gacgaacctc agcagcaacc tcctcaacga
       61 caacggcatt ccggcggctg ccaacgttcg caacaccaag gacatcaccg actacaagct
      121 catctccttc ttcgggcgat tcaactacaa catctccgac cgctacctcc tggccatgag
      181 cctgcggcgc gatgggtcct cacggttcgg ccccggcaac cagtggggca cgttcccttc
      241 cgtcgcggtg gcgtggcgga tctcgcagga gccgttcatg cggggtatcg ggtcgctgtc
      301 ggacctgaag ctgcgtgcgt cgtgggcgcg gacgggtaac caggcgtttg gcgactatct
      361 gcagtacccc acctatacct acagtgacgg tcagacgcag gtgcagttcg gcaaccagtt
      421 cgtcaccacg atccgcccga gcgcggtgga cgagttcatc cattgggaga agacgaccgc
      481 gtacaatgct ggccttgatt tcggcttcag caaccagcgc tacagcggcg cgattgacgt
      541 gtacaccaag aacaccagcg acttgatctt caatgttccc gtcgccgcgg gcacgaacct
      601 cgggaatttc gtcacgacca atatcggctc aatgcggaat cgagggctcg agctcaacct
      661 cagcgcccgg gtgatggaag ggcgcacgag cggcctgggc tggacggctt cgatcacagc
      721 cagccacaat acgaacgagc tgctcaccat caaccccagt aagagcgtat cgaagatctt
      781 gaccggcaac atctcgggag gggtcggcaa cctggtcgag gtccttgagc cgggcgtgcc
      841 ggtgaactcg ttcttcgtct atgagcagaa gtacgcgaac gggaagccgg tctacgacgc
      901 gaacccgctc aacatgtacg tggaccagaa cggggacaat gtcatcaacg actccgatcg
      961 acgcccgttc cacgacccgg cgcccaagtg gatattcggc cactcttcgt acttcacctt
     1021 cggcagccac ctggacgcca gcttcacagt gcgggcctac atcggcaact acgtgtacaa
     1081 caacgtggcg tcggccaacg gcgcctacca gaacctcacg ggttcgggga tgccgagcaa
     1141 cctgcacgcg tccgtcctgg agaccgggtt cgtcgtccca cagtactact cggatttctt
     1201 cgtggaggac gcctcgttcc tccggatgga caacatcacg gtcggctact cgttcgacta
     1261 ccagggccgg ccgttccgtc tgtacggtac cgtccaaagc gccttcaccc tcacgggcta
     1321 caacggggtg gatccgaccg ctggtctgaa cgggatcgac aacaacatct atccgcgcgc
     1381 gcgcactttc accgccggcc tgagcgtccg gttctaaggg agaccgagac atgaaccgca
     1441 caacgacagc aatgagcctc gccgtcgcgt tcctgtgcgt ggccgggatc ggctgcacgg
     1501 atccgacggt ggcaccgaag agcaccgtca ccagcgccaa cgtcttcaac gaccccaact
     1561 cgtatcgggc gttcctcgcc aagatctacg gggggctgat cgtcaccggg caacgcgggc
     1621 cagacgggaa tcccgacatc ggaggcatcg acgagggttt cggcgaatac ctgcgcctgt
     1681 actggtacct gcaggaaatg ccgacggaag aggcggtgat tgggtggaac gatcccggtc
     1741 tccccgacct gaaccggggg acttggggtg cggataacag catggtcaac gcgatgtatt
     1801 accgtgtgtt ctatcagatc atgctagtga acgagttcct gcgccaatcg accgatgcga
     1861 agctggactc ccgcggccag aacgacccgg cgttgagaac caacgtccac ttctaccgcg
     1921 ccgaggcccg gttcctccgc gccctgagct actggcacgg tatcgacctg ttcgggaaca
     1981 tcccgctggt gaccgaaacg gacccgctcg ggtccacgcc accgaagcag gccacgcgcc
     2041 agcagctcta cgactacgtc gtcggtgagc tcaacgccat catcggcgac ctgccgccga
     2101 aagggccgga gacgtacggc cgcgcgacgg cggctgcggc gcacatgctg ttggccaagc
     2161 tctatctgaa cgcgggcgtg tacaccggca cgccgaacta cgccggtgcg ctcaccgagg
     2221 cacaggcggt catcagcgcg ggctatactc tggataccag cttcgtgcac aacttccagg
     2281 ccgacaacaa cacctcgccc gagctggttt tcgtcgctcc gcaggatggc aatagcacgc
     2341 agacttgggg tggcatgacg ttcctgatcc atgccggctg cggcggcagc atgtccgcca
     2401 gcaactacgg tatcgactac tgctggggtg ggtaccgcat gaagcagcag gcctatcgcc
     2461 tgtttgcggc tggggacaag cgggcctcgt tcttctggac ggccggtcag acggattcgg
     2521 tggtcgctat cggcgacttc cagtacggga tcgccgcccc caagttcacc aacaagacgt
     2581 caacgggcag cggtggcgcg cagctcggca tggtggacac ggactttccc atcttccggc
     2641 tcgctgacgc atacctgatc tacgccgaag ccaacctgcg gggcggcggc ggcagcgcga
     2701 cggatgcgct caactacttc aacgcgctcc gattccgcgc gttcggagac tcgagcgcgg
     2761 tcctcacggc tccgcagctg acgctggaca ccctccttgc cgagcgcggc cgggagctgc
     2821 tgttcgaagc ccagcgccgg accgacctcg tgcggttcgg cctgttcgag agtggcacct
     2881 acatttgggc ctggaagggt ggcgtcgctg ggggggccgc cctggccgcc ggacggaatc
     2941 tgtatccgct cccggcgaac gagctgatcg ccaacccgaa cctgacgcag aatccgggct
     3001 acggagcctg ggagggccgt tggtcgacat gaatccccag ctccgccgct tcgactggcg
     3061 gggctgggga ttcgcgctcg cgttcgtcgc gctggcgtgc ggcgagtccg gaccgccgat
     3121 caccggcaat ccgcctccgg accggccctc gctggcgccg acctaccggc ccagcggcca
     3181 catggcctcg ggcgacgtgt tcgtgcacct cttcgaatgg aaatggaccg acatcgcgac
     3241 cgagtgcgag acggtcctcg gaccggccgg attcgcggcc gtccaggtgt ccccaccgca
     3301 ggagcacagc ctcgagcccc ccacctatcc ctggagcgag cgctaccagc cggtgagcta
     3361 cagcatcgcc cgcagccgct cgggcagcgg cgccgaattc caggccatgg tggaccgctg
     3421 cgcggccgtc ggggtcggga tctacgtgga tgccgtcatc aaccacatga cgaactaccc
     3481 cagccccggc gtaggctcga acggcaccgc atactccaag tacgactacc cgggactcta
     3541 caccccggcc gacttccacc cggcgtgcag cgtcaacaac taccaggacg ccgccaacgt
     3601 gcaggattgc gagctgctct cgctgcccga cctcaatacc ggaaagcgct cggtgcggca
     3661 gaccatcgcc ggctacctcg tcatgctggc gcgcgcgggc gtcgcgggct tccgcatcga
     3721 cgcggccaag cacatccagc aggtggagct cgacagtatc ctgcgaatcg tggactccac
     3781 catgaccgcg gaggggcggc cgatcccgta ctacttcctc gaagtgatcg gcggggcggg
     3841 cgaggccctg gcgccgcgcg actacttcgg cgaagcgtat ggctccggtg gcgcggcgga
     3901 catcaccgag ttcaccttca cgggcgtggg cgacaagttc cgcgagctgg cgggacagcg
     3961 catcgcgcag ctcaatccca acggtccgcc ggggagccag ttctccgaag tcgcctgggg
     4021 gctgatgccg tcggacaagg cggtggtgtt cctgcagaac cacgacacgc agcaccagtg
     4081 cggcctgagc taccgcgacg gtgacgtgtt ccgcctcgcg aacgtctgga tgctggcgca
     4141 gccctacggc tacccgtcca tcctgtcgag cttcgcgttc gcctgtcccg cggggaatgc
     4201 catgggcccg ccctcggacg gcagcggcaa caccaacgac gtgacctgcg cgtcgagcct
     4261 cgagaccgcg accacgggcc aatgggtgtg cgagcaccgc gacccgtcca tccggagcat
     4321 ggtgagattc cggcgcgtcg tcgccggcag tgacgtcaac cactggtggg acgacggcgc
     4381 caatgcgatc gccttctccc gcggcgccaa gggattcgtg gcgatcagcc gcgaacccgc
     4441 ggcactcgac acgacgatcg cgacgggatt ggcgccgggg acctactgcg acgtgatcac
     4501 gggcgggatg gcgagcgggg tgtgcgccgg taccgcggtc gtcgtggatt cggggcacgc
     4561 cgtcgcgctg cacctcgggc cgaactccgc cgtcgccatc gacatcgaga cacggttgcc
     4621 atgaccgccc cgatcgccct gctccaggtg accctgcatc tgaccgccgt gccggcgacc
     4681 actcccgcga ccgcgaccat gtacgtcgcc ggcacgttca acggctggaa cccgggcgcc
     4741 tacgggtaca ccctgagcgc gcagtccggg ggagggtacg ccatcacgct tcccgccacg
     4801 gtgcgcgggc gcatcgagtt caagttcacc ctcggctcgt gggagacggt cgagctggac
     4861 agcgccggtg gcgacgtctc caaccggagc ttcaccgtcc cggacagcgg ccctgccgtg
     4921 tacaccggcg cggtcatgcg atggcgcgat gggactccac ggcctcctcc ggcgcactcg
     4981 accacggcat cggtcaccgt actcgacacg gcgttcgtca taccgcagct cggccggccc
     5041 cgccgcgtct gggtctatct cccgcccggc tacgccgagt cgcgcgcacg ctaccccgtg
     5101 ctgtacatgc acgacggcca gaacgtattc gacgccgcca cgagcttcgc cggcgaatgg
     5161 ggcgtggatg aatcgctcga cagcctcgcg gcagggggcg atccgggcgt gatcgtcgtg
     5221 gccgtggacc acggcggccc gcggcgcatg gacgagtact ccccgtggcg caatccgacc
     5281 cggggcggcg gcgaggggga cgcgtacgtc gatttcctgg tgcagacgct caagccctgg
     5341 atcgaccggc actaccgcac ccgcgccgac cgggggcata cgggcgtcgc cgggtcgagc
     5401 atgggcggcc tgatttccct gtacgccgcg ctcaagtacc caaacgtttt cggccgcgta
     5461 ggcgtgttct cgcccgcgtt ctggttcgcg ccttccattt atgcctacgc tcgccgcgcc
     5521 catccgctac gggacacgcg gatcttcatt gtgatggggg gagaggaagg agacacgccg
     5581 gcggtgtacg tccgggacgc ccagcgcatg gccgacacgc tgcgggcggc ggggttcagg
     5641 gcggtgcagt cggtggtccg ccccgacggc gcccactccg aatggttctg gcgccgtgaa
     5701 tttccggccg cctatcagtg gctcttcggg gacaccgcgc cgcgcccgtc ccgttcgccc
     5761 acgcgacggt ctccgccggc ggcctggacc cgcggcgccg tgtgctacga gatcttcgtc
     5821 cgctcgttct atgactcgga cggcgacggt atcggcgacc tgaacggcct gaccaagaaa
     5881 ctcgactacg tgcgcggact gggcgccggc tgcatctggc tcatgcccgt ggccgcgtca
     5941 cccagttacc acggctatga cgtcagcgac tactacaagg tcgagcccgc ctacggcacc
     6001 accgacgatt tcaagcgttt cgtgacggaa gcgcaccgcc gcgggatcgc ggtgctggtg
     6061 gacatggtgc tgaaccacac ctcgagcgag atatcctggt tccaggccgc gctgcgcgac
     6121 acggcgtcgt cctatcgtca ctggtaccgt ttctcgccgg tgcagggcgg gaaagggccc
     6181 tggggcgccg aggcctggca ccggtccacg gtgcgcgacg agtactatta cggcgtcttc
     6241 tggagcggga tgccggacct cgactaccac acgccggccg tgcgggagga ggcgaagaag
     6301 atcgccacct actggctgcg cgagatgggg gtggatggct tccggctgga tgccgtgccg
     6361 tatctcgtgg aggacggcgc ctgcatgacg gggtgcccgg gcacgcacgc gttcctgcac
     6421 gaatacgcgg cgcacgtgga cagcgtgaaa cccggcgcgt atacggtagg ggaagcgtgg
     6481 ggcaacatcg ccaccgtgct cccgtactac ccggaccagc tgacgtccta cttcggcttc
     6541 gagctggccg actcactga
//
LOCUS       DBNGZW010000630         3146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_537535, whole genome shotgun sequence.
ACCESSION   DBNGZW010000630 DBNGZW010000000
VERSION     DBNGZW010000630.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_537535"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1335)
                     /locus_tag="AC16JC_14785"
     CDS             complement(118..1335)
                     /locus_tag="AC16JC_14785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002613759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5166014.1"
                     /translation="MLFEEIIRVALQAVRTNTLRSLLTMLGIIIGVGAVITMVALGAG
                     ARKAVEARIHTLGPTLLTIEPGQSYQQGVASEARAGLTVDDFTALDQDAHHFEAFVPE
                     LNEDYQVQYGSHNINVGITGTTPDYGPIKGYRITSGRMFSAGDDESRRRYAVLGADVP
                     DLLKANGPTLIGQEILIRGIPFEVVGILSEKGTTGSGRGNPDNDILIPLQTARYRVMG
                     HDHLRRLTGKVDDLQHMNLGMIEIERVLRRAHKIPDDRDNDFEISSQSDVIATYQETT
                     DTFGFLLASVAAVSLLVGGIGIMNIMLVSVTERTREIGVRMAIGASRRNIMLQFLVEA
                     LVLCLGGGAIGVVLGTLGAVLLSAVAHWNTAISLYAILVAFAFSASVGLFFGIWPARR
                     AAALDPIVALRYE"
     gene            complement(1372..2712)
                     /locus_tag="AC16JC_14790"
     CDS             complement(1372..2712)
                     /locus_tag="AC16JC_14790"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5166015.1"
                     /translation="MKRILWGAALIVTASACGKPAEQPAEYELIPVERRDIDLTVSAA
                     GAIQPVLTVEVKSKASGEIIGMHVQSGADVRAGQLLAEVDQRIPRNVVEQASANLDVA
                     QAQLSNAQMQVARADTLYKAQAITETEYETARLALASATAGVVRARTELRAARDALGD
                     TRLLAPVSGTIIDKYVELGTVISSPTRDVGGGTVILTMANLDTVQLQAMVDETDIGKI
                     SPGLPVRITVDAYPRRPFQGTVLKIEPKAQVRQNVTMFPVMVNIVNPGHLLKPGMNAE
                     FEVDVGHRSNVLAVPNAALRHEDDVAAAADWLGLDKKVMKRRLARGDAPRPAADGGTR
                     AERVATPPTPPAPQPASGGSAAEQKTGSSAGVSLHDASGGESRVTFVLRKGRAMPVHV
                     VVGLTDLDYTEVLSGLSEGDTVLILPTAGLVSELRKQQRKAQTRTAGGLPGLRQ"
     gene            complement(2709..>3146)
                     /locus_tag="AC16JC_14795"
     CDS             complement(2709..>3146)
                     /locus_tag="AC16JC_14795"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011524124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5166016.1"
                     /translation="EIGFVFQTFNLLPRADALHNVELPLIYAGVAPQPRREAAAMALE
                     LVGLADRMEHLPNELSGGQRQRVAIARALVHTPSILLADEPTGNLDTETGEEIMSLFE
                     QLWEAGQTIVLVTHEHDIAAHARRRIHLRDGIVEQDLITERVS"
BASE COUNT          451 a         1059 c         1111 g          525 t
ORIGIN      
        1 cgtcgcgggc ctacctcgag cgggccgggg aaacctaccg ggcgaacgcg caggccgccg
       61 gcgtggagcg cgtgcaggcc gaaatgtcac gactcggcct cgtgtgagcc gctcccgcta
      121 ttcataacgc agcgcgacga tcgggtcgag cgccgccgcc cgccgggcgg gccagatgcc
      181 gaagaacagg ccaacgctcg cgctgaaggc gaaggcgacg agaatcgcat agagcgagat
      241 cgccgtgttc cagtgcgcca ccgcggacag cagcaccgcc ccgagggtcc ccagcaccac
      301 cccgatcgcc ccgcccccca gacatagcac cagcgcctcg accaggaact gcagcatgat
      361 gttgcggcgg gacgccccga tcgccatgcg gacgccgatc tcccgggtac gctcggtgac
      421 cgacacgagc atgatgttca tgatgccgat gccgcccacc agcaggctca ccgcggccac
      481 gctggcgagc aggaagccga acgtgtccgt ggtctcctga taggtcgcaa tgacgtcgct
      541 ctgactgctg atctcgaagt cgttgtcgcg gtcgtcgggg atcttgtggg cgcgacggag
      601 cacccgctcg atctcgatca tgcccaggtt catgtgctgc aggtcgtcga ccttcccggt
      661 gagccggcgc aggtggtcgt gccccatcac gcggtagcgc gccgtctgca gggggatcag
      721 gatgtcgttg tccggattgc cgcgcccgct tccggtcgtc cccttctccg agaggatgcc
      781 gacgacctcg aagggaatcc cgcggatcag aatctcttgg ccgatcagcg tcggaccgtt
      841 cgccttcagc aggtcgggca catcggcacc gagcacggcg tagcggcggc gcgactcgtc
      901 gtccccggcc gagaacatgc gcccggaggt gatgcggtag cccttgatcg ggccgtagtc
      961 gggcgtggtc ccggtgatgc cgacgttgat gttgtgactg ccgtactgga cctgataatc
     1021 ctcgttgagc tccgggacga atgcctcgaa gtggtgggca tcctggtcca gggcggtgaa
     1081 gtcgtccacg gtgaggcccg ccctggcttc actcgccacg ccctgctggt agctctggcc
     1141 cggctcgatc gtgagcagcg tggggccgag ggtgtggatg cgggcctcca ccgccttccg
     1201 ggcgccggcg ccgagcgcga ccatcgtgat cacggcgccg acaccgatga tgatgcccag
     1261 catcgtgagc agcgagcgca gggtgttcgt gcgcacggcc tggagtgcga cgcgaatgat
     1321 ctcctcgaac agcatgcgat gcctcctgac ggggagccgg ccggcgtccc gctactggcg
     1381 caggccgggg agcccgcccg cggttctggt ctgggccttg cgctgctgct tgcgcagctc
     1441 gctgacgagt ccggcggtcg ggaggatgag cacggtgtca ccctcgctca atccgctcag
     1501 cacctcggtg taatcgagat cggtgagccc gacgacgacg tggacgggca tcgcgcggcc
     1561 cttgcgcagg acgaaggtga cgcggctctc ccccccgctg gcatcgtgga gcgatacgcc
     1621 cgccgagctg ccggtcttct gctccgccgc actgccgccg cttgccggtt ggggcgcggg
     1681 cggggtcggg ggagtggcca cgcgttcggc gcgggtgccc ccgtcggcgg cggggcgcgg
     1741 ggcgtcgccg cgcgcgagcc ggcgcttcat caccttcttg tcgagcccga gccagtcggc
     1801 ggccgcggcg acgtcgtcct cgtggcgcaa cgcggcgttg ggcaccgcga gcacgttgga
     1861 gcggtggccg acgtcgacct cgaattcggc gttcatcccg ggcttcagca gatgtccggg
     1921 gttgacgatg ttcaccatca ccgggaacat ggtgacgttc tgccgcacct gcgccttggg
     1981 ctcgatcttc aggacggtgc cctggaacgg ccggcgcgga taggcgtcca cggtaatgcg
     2041 caccgggagc ccggggctga tcttgccgat gtcggtctcg tcgaccatcg cctgcagctg
     2101 cacggtgtcg aggttcgcca tggtgaggat caccgtgcca ccgcccacgt cccgggtcgg
     2161 tgaggagatg acggtgccca gctcgacgta cttgtcgatg atggtgccgc tcacgggggc
     2221 gaggagcctg gtgtccccca aggcgtcgcg ggcggcccgc agctcggtcc gggcgcgcac
     2281 cacgccggcg gtcgcgctgg ccaacgcgag ccgagccgtt tcgtattcgg tctcggtaat
     2341 ggcctgcgcc ttgtacaggg tgtccgcccg ggccacctgc atctgggcgt tgctcagctg
     2401 cgcctgggcc acgtccaggt tggcgctggc ctgctcgacg acgttgcgcg gaatgcgttg
     2461 atccacctcg gccaggagct ggcccgcgcg gacgtcggcc ccgctctgga cgtgcatgcc
     2521 gatgatctcg cccgaggctt tggatttcac ttcgacggtc agcaccggct ggatcgcccc
     2581 cgccgcggac acggtgaggt cgatgtcgcg ccgctcgacc ggaatgagct cgtactccgc
     2641 cggttgttcg gcgggcttcc cgcacgcgct cgcggtcacg atcagggctg ccccccagag
     2701 gatgcgcttc atgagactcg ctccgtgatg aggtcttgct cgacgatgcc gtcgcgcaaa
     2761 tggatccggc gacgcgcgtg cgccgcgata tcgtgctcgt gggtgaccag gacgatcgtc
     2821 tggccggcct cccacagctg ttcgaacagg ctcatgatct cctcgcccgt ctccgtgtcg
     2881 agattgccgg tcggctcgtc ggccagcaga atgctgggag tgtggacgag ggcccgggcg
     2941 atggcgacac gctgccgctg tccaccggac agctcgttgg ggagatgctc catgcgatcg
     3001 gccagaccga ccagctccag ggccatggcg gccgcctcgc ggcgcggctg cggggcgacc
     3061 ccggcgtaga tcaacggcag ctccacgttg tggagggcat cggcgcgcgg caggagattg
     3121 aacgtctgga agacgaagcc gatctc
//
LOCUS       DBNGZW010000631         3841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1354173, whole genome shotgun sequence.
ACCESSION   DBNGZW010000631 DBNGZW010000000
VERSION     DBNGZW010000631.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3841
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1354173"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2049
                     /locus_tag="AC16JC_14800"
     CDS             <1..2049
                     /locus_tag="AC16JC_14800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166017.1"
                     /translation="VYALGCMTYEMLVGDPPFTGSTAQAIVAKVLTEKPVPPSRLRDT
                     VPTSVEDAVITALAKLPADRFASAAEFAAALSAGGTAATRAASGARPSRAGRMLWPAV
                     AALAGAVALWGWLRPRPAPPSFPPSRLAILVPELGGSSTALQRQLALTPDGSALLFTA
                     VENGRNITMRQPLDAGEPSPIPGVIPFLAGYVVSRDGREFVGSIAGSETYRYPIGGGS
                     GRPLPREIAVTSFTAWARDGSVWMSSERSTDRSWAHVTADGQVTHPFGAAHGDLNLMQ
                     LLPDDRSALAIASPPGSSSGPAALLDLAAGTTTQLIEAPIVEIRYAAGFLVYVQSDGS
                     MEAVSFDPSSRKVGDSPVTIATGVSLTGSGLAQFAVADNGTIAYIPEEPRSLVLVNRD
                     GSSRPATAQRHNYHAPKFSPDGRRILVDFTSPDGRDVWVLDRDGGGLTRVTFDRGGHD
                     AVWEPGGRSITYATVRPGVLSLFRTRPGGAEQPESLFSALQLGYTGDWLHDGSALVTV
                     GNGLDSGSLGDIAILRNGGHGPLEPLVATRFDEIYPDVSPDDQWLAFSSNQSGQVQVY
                     VRPLNGHGDQIQVSLDGGTEPMWSHDGRELFYRAGSSGAAVLMAATIQTRPVLAVTSR
                     RSLFSVGDIMTSTPHSDYDVSPDGRTFVMVRQNPAARIMVIQNLPALVERLHGAGGTR
                     "
     gene            2046..>3841
                     /locus_tag="AC16JC_14805"
     CDS             2046..>3841
                     /locus_tag="AC16JC_14805"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5166018.1"
                     /translation="MSGRSLQASLGDRYRIEREVGAGGMATVYLAHDLKHDRKVAVKV
                     LKPELAAVLGADRFLAEIKVTANLQHPNLLPLFDSGEADGFLYYVMPYVEGETLRARL
                     ERERQLPVDETVRLVTLLANALDYAHAHGVIHRDLKPENILLQAGQPVIADFGIALAV
                     AQAGGSRVTETGLSLGTPHYMSPEQAAGDRDLDARSDQYALGAVTYEMLTGEPPHTGP
                     TAQIIIARLMTEKPRSLRATRPGVPVAMDLAIGRALAKPPADRFPTCGAFARALVTSP
                     ARRNRSVWIGAAAAGLVVMAGVAWLAASRKSAVHGADVKSIAVLPLANLSGDQADDYF
                     GIGLSEEITRALAKAGVRVIGRVSAGALQAKGLDERAIARELGVSSLLTGTVQRAAGQ
                     VRINVTLVSAADGAVRWTEKYDRPLANVFAVQDEIARTVADTLLGSLGRPPSALATRA
                     ETNDPEAHALFLQGQVLFNRRTARTLNQAIALFERAAARDPTYARAEASLAMALSVLP
                     AYVQDSTTPIVTRAVAAAERAIAMDSTIAESYTALGYGYSLLGELSRAEESFRRAVAL
                     DSTVATAWGWYGLLANRLGDFRAAHERIARARG"
BASE COUNT          556 a         1349 c         1356 g          580 t
ORIGIN      
        1 gtctacgcgc tgggctgcat gacctacgag atgctggtgg gcgatccacc gttcacgggg
       61 agcaccgcgc aggccatcgt cgccaaggtg ctgaccgaga agccggtgcc gccgtcccgg
      121 ttgcgcgaca cggtcccgac ctccgtcgag gatgcggtca tcacggcgct ggccaagctg
      181 ccggcagacc gctttgcgtc ggctgcggag ttcgcggcgg cgctgagcgc gggcggcacc
      241 gcggccaccc gggccgcgtc cggggcccgc cccagtcgtg cgggccgcat gctctggccc
      301 gcggtggccg cgctggccgg cgcggtggcg ctctggggct ggctcaggcc gcgaccggcg
      361 ccgccgtcgt tccccccttc gcggctcgcg atcctcgtcc ccgagctggg cgggtcgagc
      421 accgcgctgc agcggcagct cgccctcaca cccgacggca gcgcgctgct ctttaccgca
      481 gtcgagaacg ggcggaacat cacgatgcgg cagccgctcg acgccggcga gcccagcccc
      541 atccccggcg tgatcccctt cctcgcggga tatgtcgtgt cccgggacgg gcgggagttc
      601 gtcggctcga tcgccggcag cgagacctat cgctacccga tcggcggggg cagcggccgg
      661 ccgttgccgc gcgagatcgc cgtgacctcc ttcacggcat gggcgcggga tggctccgtg
      721 tggatgagct cggagcggag caccgaccgg agctgggcgc acgtcacggc cgacgggcag
      781 gtcacccacc ccttcggcgc cgcgcacggt gacctcaatc tcatgcagct cctgccggac
      841 gaccgctcgg cgctcgccat cgcctcgccg ccggggtcat cgtcgggccc cgccgcgctg
      901 ctggatctcg cggccgggac gacgactcaa ctgatcgagg cgcccatcgt cgagatccgc
      961 tacgcggcgg gattcctcgt ttacgtgcag tcggacggaa gcatggaggc ggtctcgttc
     1021 gacccgtctt cccggaaggt cggagactcg ccggtcacca tcgccaccgg ggtgtcgctg
     1081 acggggtcgg gactcgccca gttcgccgtg gcggacaacg gcacgatcgc ctacatcccc
     1141 gaagagccgc ggtcgctcgt cctggtgaac cgggacggca gcagccgccc cgccacggcg
     1201 cagcggcaca attaccacgc gccgaagttc tcgcccgacg gccgccggat cctggtggat
     1261 ttcacatcgc ccgacgggcg ggacgtatgg gtcctcgatc gcgacggcgg cgggctgacc
     1321 cgcgtgacct tcgaccgggg cgggcacgac gcggtctggg agccgggagg ccgatcgatc
     1381 acctatgcga cggtgcggcc gggcgtcctg agtctcttcc gcacccggcc tggcggcgcg
     1441 gagcagcccg agtcgctctt ctcggcgctg cagctggggt acacgggcga ctggctccat
     1501 gacggcagcg cgctggtgac cgtgggcaac ggcctggact cggggagcct gggcgacatc
     1561 gccatcctgc gcaacggcgg ccacggtccg ctcgagcccc tcgtggccac gcggttcgat
     1621 gagatctatc ccgatgtctc tccggacgac cagtggctgg cgttcagctc gaaccagtcc
     1681 gggcaggtac aggtgtacgt gcgcccgttg aacggccacg gtgaccagat ccaggtgtcg
     1741 ctcgacggag gaaccgagcc gatgtggagc catgacgggc gcgagctgtt ctatcgcgcc
     1801 ggcagctcgg gggcggccgt cctgatggcg gccacgatcc aaacgcggcc ggtcctggcc
     1861 gtgacgtcgc gccggagcct cttttccgtg ggcgacatca tgacctcgac gccgcacagc
     1921 gactacgacg tctcacccga cgggcgcacc ttcgtgatgg tgcggcagaa ccccgcagcg
     1981 cgcatcatgg tcatccagaa tctgccggcg ctggtcgagc ggctgcacgg agcgggtggc
     2041 acgcgatgag cgggcgcagt ctccaagcct ccctcggcga ccgttaccgc atcgagcggg
     2101 aggtgggtgc gggcgggatg gccaccgtgt atctcgccca cgatctgaag cacgaccgga
     2161 aggtcgcggt gaaagttctc aagcccgagc tggccgcggt gctcggcgcc gaccggttct
     2221 tagccgagat caaggtcacc gccaacctgc agcatcccaa cctgctcccg ctgttcgact
     2281 ccggggaagc agatggcttc ctgtactacg tcatgcccta tgtggagggc gagacgttgc
     2341 gcgctcggct cgaacgcgag cgacagcttc cggtagacga gacggttcgc ctggtcacgc
     2401 tgctcgccaa tgcgctggac tacgcgcacg cgcacggcgt catccatcgc gacctcaagc
     2461 cggagaacat tttgctacag gccgggcaac cggtgatcgc ggacttcggc atcgcgctgg
     2521 ccgtggcgca ggctggtggt tcccgcgtca cggaaaccgg gctctcgctc ggcaccccgc
     2581 actacatgag cccggagcag gcggcgggtg accgggacct cgatgcgcgg tccgatcaat
     2641 acgcgttggg cgccgtgacc tacgaaatgc tcaccggcga acctccccac accgggccga
     2701 ctgcgcagat catcatcgct cgacttatga ctgagaagcc gaggagcttg cgcgcgaccc
     2761 gacccggcgt gccggtggcg atggatctcg ccattggaag ggcgctggcc aagccaccgg
     2821 ccgaccggtt cccgacctgc ggtgcattcg cgcgcgcgct cgtcacaagt cccgcacggc
     2881 gcaatcgcag tgtgtggatc ggcgcggcgg ccgccggtct ggtcgtcatg gctggtgtgg
     2941 cgtggctggc ggccagccgg aagtccgccg ttcacggtgc ggacgtcaag tccatcgcgg
     3001 tcctgccgct cgccaacctg agcggcgacc aggccgacga ctatttcggc atcgggctct
     3061 cggaggaaat cacccgggcg ctggccaagg ccggcgtgcg cgtcatcggg cgggtgagcg
     3121 ccggcgcgct ccaggccaag ggactcgacg agcgcgccat tgcccgggag ctcggcgtca
     3181 gttcgctgct caccggcacg gtccagcgcg ccgccggcca ggtgcgcatc aacgtcaccc
     3241 tggtctcggc ggcggacggg gcggttcgct ggaccgagaa gtacgaccgg ccgctggcga
     3301 atgtgttcgc ggtgcaggat gagatcgcgc gaaccgtggc ggacaccctg ctcgggtcgt
     3361 tgggccgtcc gccgtctgct ttggcgacga gagcggaaac caatgatccc gaagcccacg
     3421 ccctgttcct gcaggggcag gtgctgttca accggcgcac ggcccggacc ctgaaccagg
     3481 cgattgccct gttcgaacgc gccgcggcgc gggatccgac gtacgcccgc gcagaagctt
     3541 cattggcgat ggccctgtcc gtgctccccg cgtacgtgca ggacagcacc accccgatcg
     3601 tgacccgagc cgtcgccgcg gccgagcgcg cgatcgcgat ggactcgacc atcgccgaat
     3661 cgtacaccgc cctgggttac ggatactccc tgctgggcga gctgagccgg gctgaggaga
     3721 gcttccgccg cgctgtggcg ctcgactcca cggtggcgac ggcgtggggc tggtacggac
     3781 tcctcgccaa ccggttgggc gacttccgcg ccgcgcacga gcggattgcg cgggcgcggg
     3841 g
//
LOCUS       DBNGZW010000632         3173 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1066729, whole genome shotgun sequence.
ACCESSION   DBNGZW010000632 DBNGZW010000000
VERSION     DBNGZW010000632.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1066729"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1295
                     /locus_tag="AC16JC_14810"
     CDS             <1..1295
                     /locus_tag="AC16JC_14810"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5166019.1"
                     /translation="CGNASRAAAPPPPPVVRAEVVVEQDVPVSAEWVGTLVGSINAQI
                     RPRVSGHLVSQNYKEGAVVKAGDLLFQVDPRPYQVAFDQAGAQLLQAQSQLSQAKAQV
                     SASQAQVEQALAKVVQADAEVTKVVADQRRTELNVARYTPLAQRGFVSQQELDNAVQY
                     NLANIASVAAAHASVQNAQASVANARAALEKAQADVKTQEANIAAARAALANANLNLG
                     YTRVLAPISGLAGFRVANIGDYVGPNDQNPLTTVSQADPIYAEFPIGEQRAVEMFRRW
                     DSGGTGSHGFELELILADGSVYPTRGRAVALDRQVDVTAGTVLARGVFPNPGNMLRPG
                     QYAKVRAVVETKKNALTVPQRAIQNVQGMQQIAVVRSDDTVDVRAVKVEARVGTVAVI
                     AEGLRPGERVIVEGGDRVRPGQKVHTGSGAPAQADAGK"
     gene            1306..>3173
                     /locus_tag="AC16JC_14815"
     CDS             1306..>3173
                     /locus_tag="AC16JC_14815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011698318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5166020.1"
                     /translation="MARFFINRPIVAMVIAVLTFLVGLIALVQLPISLYPDIAPPEIL
                     LTARYPGADAVTLEQSVATPIEEQMSGVDKMLYMYSLNQGSAAQTELRVAFDVTTDPS
                     VDQVLTNLRYSQAASQLPSDVVNQGVTVKKSVSSPLGVFVLYSPKGTYDPLFLANYAY
                     VNINDPMTRVPGVGQVIIFGPSKYAVRVWVNPDKLAKLDITVNDITSALQAQNTVNPA
                     GQLGGDPTPSAQEFTFTVRAQGRLARLTDFENVVVRARPDGSLVRLKDVGRVELGAQD
                     YVRRGRLNGKPAVVVAIYQLPGSNAIETMRGATKLMDEMKARFPADLDYVTALDTTLA
                     VSEGMKEIVKTLVEALILVVIVVFIFLQGFRATLIPTLAVPVALVGTFMVFPLLGFSI
                     NTLSLFGLVLAIGLVVDDAIVVVEAVEHHIEKGLSPRDAAFKAMEQVSGPVVAIALIL
                     AAVFVPTAFIPGITGRMYQQFAVTIAVAVLLSAFNALTLSPALCALLLRPRTEMRGPL
                     GAFFRGFNRWFARATDRYVGACGHLIRKSALSMVLLGVVVGAAGLVGSRLPGGFVPEE
                     DQGYVYVNVQLPLAASLDRTAAVSDKLDEIFKTTPGIKYYTGIAGFSLLSQVTTTY"
BASE COUNT          506 a         1089 c         1060 g          518 t
ORIGIN      
        1 gatgtggcaa tgcttcacgt gcggccgcgc cgccaccgcc gccggtggtc cgggctgaag
       61 tggtggtcga gcaggacgtc ccggtctccg ccgagtgggt gggtacgctc gtcggctcca
      121 tcaacgcgca gatccgtccg cgcgtcagcg gccacctcgt gagccagaac tacaaggagg
      181 gggcggtggt gaaggcgggc gatctcttgt tccaggtcga tccgcggccg taccaggtgg
      241 cgttcgacca ggcgggggcc cagctccttc aggcgcagtc gcagctgagc caggccaagg
      301 cgcaggtcag tgcgagtcag gcccaggtcg agcaggcgct cgcgaaggtc gtccaggccg
      361 acgccgaggt cacgaaggtc gtggccgatc agcggcggac cgagctcaac gtcgcccgct
      421 acactccact ggcccagcgc ggattcgtga gccagcaaga gctcgacaac gcggtccaat
      481 acaacctcgc gaacatcgcc tccgtcgcgg ccgctcacgc ctcggtacag aacgcgcagg
      541 ccagcgtggc gaacgcgcgc gctgccctcg agaaggcgca ggcggacgtg aagacgcagg
      601 aggccaacat cgccgctgcg cgggccgcgc tcgccaacgc gaacctcaac ctcggctaca
      661 cgagggtgct cgcgccgatc tccggcctgg cgggtttccg tgtggccaac atcggcgact
      721 acgtcggccc gaacgatcag aacccgctga cgacggtctc ccaggccgat ccgatctacg
      781 cggagtttcc gattggcgag cagcgcgcgg tcgagatgtt ccgccgctgg gactcgggcg
      841 gcaccggctc tcacggcttc gagctcgagc tgatcctcgc ggacggcagc gtgtatccca
      901 cccgcgggcg cgccgtcgcg ctggatcgcc aggtcgacgt aacggccggc accgtgctgg
      961 cgcggggcgt gtttccgaat cccggcaaca tgcttcgccc cggccagtac gccaaggtgc
     1021 gcgccgtcgt cgagacaaag aagaatgcgc tgaccgtgcc ccagcgcgcc atccagaacg
     1081 tccagggcat gcagcagatc gcggtcgtcc ggtccgacga caccgtggac gtgcgcgcgg
     1141 tgaaggttga ggcacgcgtc ggcaccgtgg ccgtcatcgc cgaggggctc agacccggcg
     1201 agcgtgtgat cgtcgaaggc ggcgatcgcg tgcggccggg tcagaaggtt cacactgggt
     1261 cgggcgcgcc ggcacaggcc gacgccggaa aataggggtc acatcatggc gagattcttc
     1321 atcaaccgtc cgatcgtcgc gatggtgatt gcggtcctga cgttcctcgt cggcctcatc
     1381 gccctcgtgc agctccccat ctcgttgtat cccgatatcg cgccgcccga gatcttgttg
     1441 accgcgcggt atcccggcgc cgacgccgta acgctcgagc agtcggtggc cacgccgatc
     1501 gaggagcaga tgagcggcgt ggacaagatg ctctacatgt actcgctcaa ccagggcagc
     1561 gcggcgcaga ccgagttgcg ggtggcgttc gacgtcacca ccgaccccag cgtggaccag
     1621 gtcctgacga acctccgcta ctcgcaggcg gcctcgcagc tgccgtccga cgtggtgaac
     1681 cagggcgtca cggtcaagaa gtcggtcagc agcccgctgg gcgtcttcgt gctgtactcg
     1741 cccaagggca cgtacgatcc gctcttcctc gctaactacg cgtacgtcaa catcaacgat
     1801 ccgatgacgc gcgtgcccgg tgtcgggcag gtcatcatct tcggaccgtc gaagtacgcc
     1861 gtgcgcgtgt gggtcaatcc ggacaagctg gcgaagctcg acatcacggt caacgacatc
     1921 accagcgcgc tacaggctca gaacaccgtc aacccggccg gccagctcgg cggggacccg
     1981 accccgtccg cgcaggagtt tacgttcacc gtgcgggcgc agggccgctt ggcgaggttg
     2041 accgacttcg aaaacgttgt ggtccgcgcc aggccggacg gctccctggt tcggctcaag
     2101 gacgtgggcc gcgtggagct gggggcgcag gactacgtcc ggcgcggccg cctgaacggc
     2161 aagcccgccg tcgtcgtcgc catctaccag ctccccggct ccaacgccat cgaaacgatg
     2221 cgcggggcga caaagctgat ggacgagatg aaggcccggt tccccgccga cctcgactac
     2281 gtgacggcgc tcgacacgac tctcgcggtg agcgagggca tgaaggagat cgtcaagacc
     2341 ctggtcgagg cgctgatcct cgtcgtcatc gtggtcttca tcttcttgca gggcttcagg
     2401 gccacgctga tcccgacgct cgcggtgccg gtggccctgg tcggcacgtt catggtgttc
     2461 ccgcttctcg gcttctccat caataccctg tcgctgtttg ggctcgtgct cgccatcggc
     2521 ctcgtggtgg acgacgccat cgtcgtcgtc gaggcggtcg agcaccacat cgagaagggg
     2581 ctctcgccgc gcgacgccgc cttcaaggcg atggagcagg tctcgggacc ggtggtggcg
     2641 atcgcgctca tcctggccgc cgtcttcgtg ccgaccgcct tcatcccggg aatcaccggc
     2701 cggatgtacc agcagtttgc ggtgacgatc gccgtggcgg tgctcctctc ggcgttcaac
     2761 gccctcaccc tgagcccggc gctctgcgcg ctgctgctgc ggccgcggac ggagatgcgc
     2821 gggccgctcg gcgcgttctt ccgcggcttc aaccgctggt ttgctcgtgc caccgatcgg
     2881 tacgtcggcg cctgcgggca cctcatccgc aagtccgccc tctcgatggt cctgctcggc
     2941 gtcgtcgtgg gcgccgccgg cctcgtgggc agccggctgc ccggcggctt cgtgcccgag
     3001 gaggaccagg gctacgtcta cgtcaacgtc cagctccccc tggccgcgtc gctcgatcgc
     3061 acggccgcgg tcagcgacaa gctggacgag atcttcaaga ccaccccggg catcaagtat
     3121 tacacgggca ttgccggttt cagcctgttg agccaggtga cgaccacgta caa
//
LOCUS       DBNGZW010000633         3576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1066738, whole genome shotgun sequence.
ACCESSION   DBNGZW010000633 DBNGZW010000000
VERSION     DBNGZW010000633.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1066738"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1495
                     /locus_tag="AC16JC_14820"
     CDS             170..1495
                     /locus_tag="AC16JC_14820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166021.1"
                     /translation="MRRAVVLAGSVVGLLAGAAPARAQLSFDARRTGMAGVSLSRDGA
                     LGHYNPAYQSVPARTGRSAKLTIPIPLGLFQVLKDSAAFDVHKSYFNAIALAEYVMHP
                     PLFLEVKKVPEPTNDVEFTIGRNALLVDLGSSAQLVPTDRFGFGGESRLMDIGPTIKG
                     VHAGVVVWEDHEVGFQLDSALLGFLRLQDSARTNTLYGSDGDGLVQGGLAPTLGYSGR
                     LWGDSSRGIYAGAALHYYLGAAYGSVRGTGGFLTGDTLFAGSNPVTPTVDARVQYSKA
                     GNSLGHGFGADIGFVYVTGPVEVGLGVNNLGATLTWSDTRADSIYLDTVTNVLASQFV
                     ANHFRSKTRLPVTYVANASIELGTNTTAGAMLLDNGRGTSIQLGVEQRVGPLALRGGV
                     ARDQRKRVQVGWGTGVRFGPLSLDVGFLTHSSALSSARGITMATSLSIY"
     gene            1495..2076
                     /locus_tag="AC16JC_14825"
     CDS             1495..2076
                     /locus_tag="AC16JC_14825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166022.1"
                     /translation="MPMPPMPLTAPVPARRRAYWLAAAGLALAMGCGQGHLIFDVDVY
                     SFLKGVGQDSLPYLAPSPLADTIPVQQVNGLATGLGSSVVESVTVSGSVDFVNATGSG
                     MVGLQIYLDTVPTLYGGPAAVGVPPVAVTPGATSTTSFQDTVPAILRQRLLNTKLYVG
                     MRVTAVATSGTVTGKARLSALRMRIVVQDNLAP"
     gene            2163..>3576
                     /locus_tag="AC16JC_14830"
     CDS             2163..>3576
                     /locus_tag="AC16JC_14830"
                     /inference="COORDINATES: protein motif:HMM:NF020698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5166023.1"
                     /translation="MSESPVGHYAGPRRMSRRGLAALLSAAVLATCTSDRAPTGPGAG
                     GRGYLSIRPVLAAPANLAAFGFTIDSLRVAAIRPATDTVVDTTVFFDPNSATLKLALS
                     VPLTSSPETFFLLLQLRAGTQVLYSGVDTTQVSAGAPDTSSSASVSLKYVGPGAGLTS
                     ISIAPRDSVVTLLGTKTFRVSADSNGKAISSFYVSWSTSDTLTAPIDSLGVLHAPAAR
                     ASMFVRAVTPDGVKDSTRVTFIPVPTAVTAFSGGGQTGAVTTRLPLPLRVQVTAADLL
                     GVKGVPVRFQALTGGGSVRDSVVITDSLGFAEDSASLGTLAGAATFQASVTGLTPAAF
                     AATATAGAISAAQSVVTVSAPTLASGAAATLTLQAKDAFGNNLTGGGAIVVFTASGGT
                     STGSIGSTTDHGNGSYTATFTGVVAGTAVTIGATINGTAVTTTPPTLTVIAGGIAAAS
                     SIVSVSSATVASGGSITLTLQ"
BASE COUNT          495 a         1312 c         1205 g          564 t
ORIGIN      
        1 gggcagggca ccaccgtccg tctggtgctg cagccggcgc ccccggcgcc cccggcgcct
       61 ccggccgccc cgggagcacc gcgccggact ggtgcgcggt gacccgtctc acgacattgt
      121 agcccgtcgg atgccaccat tccttcctgc tttccttatg aggtggatcg tgcggcgtgc
      181 cgtcgtgctg gccgggtcgg tcgtcgggct cctcgccggg gcggcccccg cgcgtgccca
      241 gctgtcgttc gatgcgcggc gcaccggcat ggcgggcgtc agtctcagcc gcgacggcgc
      301 gctcggccat tacaaccccg cgtatcaaag cgtcccggcg cgcaccgggc ggtccgcgaa
      361 gctcacgatt ccgatcccgc tcggcctgtt ccaggtcctg aaggacagcg cggcgttcga
      421 cgtccacaag tcctacttca acgcgatcgc gctggcggaa tacgtgatgc acccgccgct
      481 cttcctcgag gtcaagaagg ttcccgagcc caccaacgac gtcgagttca cgatcggccg
      541 caacgccctg ctcgtggacc tcggctcgtc ggcccagctc gtgcccacgg accggttcgg
      601 gttcggcggg gagagccgcc tcatggacat cgggcccacc atcaagggcg tgcacgccgg
      661 cgtggtcgtg tgggaggacc acgaggtggg gttccagctc gacagtgcgc tgctcggatt
      721 cctgcgactc caggactccg cccgtacgaa taccctgtac gggagtgacg gcgacggcct
      781 cgtgcaaggc gggctcgcgc cgacgctggg ctactccgga cgcctctggg gtgacagctc
      841 gcgcgggatc tacgccggcg ccgcccttca ctactacctc ggcgcggcct acggctccgt
      901 ccggggaacg ggcggctttc tgaccggcga cacgcttttc gccgggtcga accccgtcac
      961 cccgaccgtc gatgcccgcg tgcagtactc caaagccggc aactccttgg gacacggctt
     1021 cggcgccgac atcgggttcg tgtacgtgac cggcccggtc gaggtcggcc tgggcgtcaa
     1081 caacctcggc gctaccctca cctggtcgga cacccgcgcc gacagcatct acctggacac
     1141 cgtgacgaac gtgctcgcct cccaattcgt ggcgaatcac tttcggagca agacgcggct
     1201 cccggtcacc tacgtggcca atgcctcgat cgagctgggc accaacacga ccgcgggggc
     1261 gatgcttctc gacaatggtc gcggcacgag catccagctg ggcgtggaac agcgcgtcgg
     1321 gccgctggcg ctgcggggcg gggtggcgcg cgatcagcgc aagcgggtgc aggtcggctg
     1381 gggaacgggg gtgcgcttcg ggccgctgag cctcgacgtc ggctttctca cccattcgag
     1441 cgcgctctcc agcgcgcgcg ggatcacgat ggccacctcc ctctcgatct actgatgccc
     1501 atgcccccca tgcccctgac tgctcctgta ccggcacgcc gacgcgcata ctggctggcc
     1561 gccgccggcc tggcgctcgc gatgggctgc gggcagggtc acctgatctt cgacgtggac
     1621 gtctacagct tcctcaaggg tgtggggcag gactcgctgc cgtatctcgc cccctcaccc
     1681 ctggcggaca ccattccggt gcagcaggtg aacgggctcg cgaccggtct gggctcatcg
     1741 gtcgtggagt ccgtcaccgt cagcgggtcg gtggacttcg tcaacgcgac gggcagcggg
     1801 atggtcgggt tgcagatcta cctggatacg gtgccgaccc tgtacggcgg tccggcggca
     1861 gtcggagtgc cccccgtggc cgtcacgccc ggggccacca gcacgacgtc gttccaggac
     1921 acggtgccgg cgatcctgcg gcagcgtctg ctcaacacga agctctacgt cggcatgcgg
     1981 gtcaccgccg tggccacctc gggaacggtg actgggaagg ctcggctgag cgcgttgcgc
     2041 atgcgcatcg tggtccagga caacctggct ccctgaagcc gggcgccccg acgaccgtcc
     2101 cggcgctcta gcctcccgag gcgccggatt gcagattcca gcccaccaat gacgctcctc
     2161 tggtgagcga gagcccggtt gggcattacg cggggccgcg gcggatgagc cgccgcggcc
     2221 tcgcggcgtt gctgtcggca gccgtcctgg cgacctgcac ctcggaccgg gcccccaccg
     2281 ggccgggagc gggcgggcgc gggtacctgt cgatccgtcc ggtgctcgcc gctcccgcaa
     2341 acctcgccgc attcggcttc accatcgaca gcctgcgcgt cgccgccatt cgcccggcga
     2401 ccgacaccgt cgtggacacc acggtgttct tcgatcccaa cagcgccacc ctcaagctgg
     2461 cgctttcggt gccgctcacc tcgtctcccg agacgttctt cctgttgctc cagctgcgcg
     2521 ccggcaccca ggtgctctac agcggcgtcg acacgacgca ggtgtcggcc ggagcgccgg
     2581 acacgtcgtc cagtgcatcc gtgagtctca agtatgtcgg tcccggcgcg gggctgacca
     2641 gcatcagcat tgctccgcga gactcggtgg tcacgctgtt gggcacgaag acgttccggg
     2701 tcagcgccga ctccaacggc aaggcgatca gttcgttcta cgtgagctgg tcgacgagcg
     2761 acaccctcac cgccccgatc gacagcctgg gtgtgctcca tgcgcccgcc gcgcgcgcca
     2821 gcatgttcgt gcgcgccgtc acgcccgacg gcgtgaaaga ctccacgcgc gtcacgttca
     2881 tccccgttcc caccgccgtg accgccttct cgggcggagg gcagacgggg gcggtcacca
     2941 cgcgactacc gttgccgctg cgcgtccagg tgaccgcggc cgacctcctc ggcgtgaagg
     3001 gcgtgccggt ccggttccag gcgctcacgg gcggcggcag cgtgcgcgac tcggtcgtga
     3061 tcaccgacag cctgggattc gcggaagatt ccgcgtcgct cgggacgctg gccggcgctg
     3121 cgacgttcca ggcgagcgtc accgggctca ccccggctgc gttcgcggcc accgccaccg
     3181 cgggcgccat ctcggccgcc caatcggtcg tgaccgtttc ggctcccacg ctcgcctccg
     3241 gggccgcggc gaccctcacg ctgcaggcga aggacgcgtt cggcaacaac ctgacgggcg
     3301 gcggcgcgat cgtggtgttc acggcgtcgg gcgggaccag cacgggctcg atcgggtcca
     3361 ccaccgacca cggcaacggc agctacacgg cgacgttcac cggcgtcgtg gcgggcaccg
     3421 ccgtcacgat cggcgcgacg atcaacggca cggcggtcac cacgacgccg ccgaccctga
     3481 ccgtcatcgc gggcggcatc gccgccgcca gctcgatcgt gagcgtgtcg agcgcgaccg
     3541 tggcgtccgg cgggtcgatc acgctgacgc tgcagg
//
LOCUS       DBNGZW010000634         6046 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1354489, whole genome shotgun sequence.
ACCESSION   DBNGZW010000634 DBNGZW010000000
VERSION     DBNGZW010000634.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6046)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6046)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6046
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1354489"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="AC16JC_14835"
     CDS             <1..618
                     /locus_tag="AC16JC_14835"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5166024.1"
                     /translation="TLLRTRAEVAMGQERVAERDAETLRAVGREAERLGAIVGDLLTL
                     ARADAGERPAAAKPVYLDDEVAGAVESIRPLAERAAVTLEVGTFEEAPVTGDPVLLRR
                     LLVILLENAVKFTPTGGRVRLDIAGENGRRSVVITDTGIGISSEDLPRVFERFYRGET
                     ARATEGAGLGLAIARWIADMHGARIDIRSEPGQGTRVEVSFPASV"
     gene            657..1913
                     /locus_tag="AC16JC_14840"
     CDS             657..1913
                     /locus_tag="AC16JC_14840"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR5166025.1"
                     /translation="MQRRHHLTLLLLSATWLAPLGAQERLNLEDALREAHAANAQLPL
                     AAFETRIASAALREARGALGPRFSLDGDLHGGRPAEYAANDGRLQLLAEQPIYDGGAL
                     RAGVAMQRALAGAGQARYRQAEKDLDFDVRVGFAELLELDSVIAVRKAGIARLQSYRA
                     AIEARRAAGEGVTGDLLRTQVQLGQAEADLADATRQRDLARLSLNDLLGRDPTAPLTL
                     AEEAAPSTPPADTIAEAWSGTPEVRQAEAQVAAARSGTSLAAAERRPQLDLVVNAGTQ
                     PAFETSGGAFNNGQGSGVEFLLSFSWPLLDAGGYRARRDQAQLRLAEAQAGEIAVRRQ
                     ARLVWAAARAELAARYGEVETRRRTAGTALDSYLSSESLYRGGAATALEVLDAYGTWI
                     AASEAVAGAVRSYRVAAAQLERWGQP"
     gene            1910..2896
                     /locus_tag="AC16JC_14845"
     CDS             1910..2896
                     /locus_tag="AC16JC_14845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5166026.1"
                     /translation="MTRWIVVLVLLAGCSGGDQQRESAAASRDTSPEGAATPVRTAAI
                     RLATLALTIRAPGHTDVLRPLRVRAPFSGTVATLHVADGDRVARGATLGTFVSRNSAA
                     ALDGARAMLAAARTEAERQDAHRAVDLATRGLVEVPLHASEAGVVVSHAANAGDLVTE
                     GDDILVIAPAGAVAFIAQVVQTDLPRVQPGQRVAVDLAARAQPLAGTVHGILPAASAE
                     NLSAPVRIDLSAGGAVGTGLFGTARITVGERRQVPVVPDAALLQDDVYGTTQVAIVGP
                     APDHRAHWVSVRPGVRDGGLVEILAPSLPPGTTVIVSGQVGLPEGAPVRAEP"
     gene            2968..5934
                     /locus_tag="AC16JC_14850"
     CDS             2968..5934
                     /locus_tag="AC16JC_14850"
                     /inference="COORDINATES: protein motif:HMM:NF013069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5166027.1"
                     /translation="MYAAARLPASIFPSVTFPIVKVIADAGEEPAARMMPTVTRPLEE
                     AILRVPGVRLVRSTTSRGSSEISAEFAWGTDMQVALQRVQAETQRIRPDLPAETRIDV
                     EWMNTAIFPIQGYALTSSTRSQAELLALAEFTLKPALIRIPGVSEVQIQGGRRREFEV
                     WLDPGQLAARRLAPADVVETVRRSHDVLSAGLAEQNHELYLTLVDGRVEDLAGLSRIA
                     VPVESGPPATLGELGEIKVADEVSYIRTTADGEPAVLLNLIRQPSANTVAIAAGVEQL
                     FREQPQLLPSDVRWTTFYDQARFVTDSVHGTRDAILIGIVLAALVLLVFLRSLRLALV
                     AVAAIPLTVAIVGLALGIAGQTVNLMTLAGVAAAIGLVADDAIVVVEHIERHRAAARG
                     LTDLLPAIVGSSLSTTVILLPFALLAGIVGAFFKPLALTMALALGTSFLVAALVVPVT
                     IGLVGAGKERAGAAAAPERASRFLSGYERLVAACIRHGRLTAAALGGCVVAMILLYRG
                     VGTDFLPEMDEGAIILDYWTPPGTSLTDTDAMLDEAEKVIRALPDVTSYSRRTGTQLG
                     FFITEPNRGDYVITLKPRGERRDIETVMDELRDRIQAVEPAIRTDFGQLLEDNIGDLT
                     GGVPQPIDIKIFGTDPAQLQVKARQIARILDSVPGMEDTFDGIVIAGPALNIRVDAPA
                     AARYGLSTADIHAAVEPAVTGTVVDQIRVGDRMYDLRVFMRHVGVLGDLPVRAASAPA
                     ALIPLSTIATVGTGAPEAEIDRENLKTFIGVTARLSGRDLGGAVADIQRRIARNVRLA
                     PDMSIQYGGQYQQQEDSFRGLFYVLLGSLVLVSLVLLFEFGDWRAPVVTSACAVAVLA
                     GVLLALVLTGQTLNISSYVGAIMMVGIVGENAIFVIHEGRLELGRGTAPAQAWARVSR
                     QRLRPVAMTILATAFALTPLAVALGHGSQLMQPLAIAVIGGFVLSGPVVLIVLPSLYC
                     LLDPGGRLGWGGTQFQ"
BASE COUNT          833 a         2062 c         2196 g          955 t
ORIGIN      
        1 acgctgctgc gcacccgtgc cgaggtcgcc atgggacagg agcgcgtggc ggagcgggac
       61 gcggagacgc tgcgggccgt gggccgcgaa gcggagcggc tcggggccat cgtcggggac
      121 ctgctcaccc tcgcacgcgc ggacgccgga gagcggccgg ccgccgccaa gccggtctac
      181 cttgatgatg aggtggccgg cgctgtcgag tcgatccgtc ccctggccga gcgcgccgcg
      241 gtgacgctcg aagtcgggac attcgaggag gcgcccgtca ccggcgaccc ggtgttgttg
      301 cggcgcctgc tggtcatcct gctcgagaac gccgtcaagt tcacccccac cgggggccgc
      361 gtacgactcg acattgcggg ggaaaacggg cggcggtcgg tcgtgatcac cgacacgggg
      421 atcgggattt cgtcggagga cctgccccgc gtgttcgagc ggttctaccg gggggagacg
      481 gcgcgggcca ccgagggcgc cggcctcggc ctcgccatcg cacgctggat cgcggacatg
      541 cacggagccc ggatcgacat tcggtcggag ccggggcaag ggactcgcgt cgaggtgtcg
      601 ttccccgcat ccgtgtaagg tcgctgtcag cttctctgcc taccttcttc acctccatgc
      661 aacggcgtca ccacctcacc cttcttctgc tctccgccac ctggctggca cccctcggcg
      721 ctcaggagcg gctcaacctg gaggatgcgc tccgcgaagc gcacgcggca aacgcgcagc
      781 tgccgctcgc cgcgttcgaa acacggattg cctccgctgc gttgcgggag gcccgtgggg
      841 cgttagggcc tcgcttctcg ctcgacgggg atctccacgg cggccgcccg gcggagtacg
      901 cggcgaacga cggccgctta cagctcctgg ccgagcagcc catatacgac ggcggcgcgc
      961 tgcgggcagg ggtcgccatg cagcgggcgc tggcgggagc gggccaggcg cgctaccggc
     1021 aggcggagaa ggatctcgac ttcgacgtac gggtgggctt cgccgagctg ctcgagctcg
     1081 acagcgtcat agccgtgcgg aaggccggga tcgcccggct tcagtcctac cgtgccgcga
     1141 ttgaagcgcg gcgggctgcg ggagagggtg tcaccgggga cttgctgcgg acgcaggtgc
     1201 agctcggcca ggccgaagcc gacctggcgg atgcgacgcg gcagcgcgac ctggcgcgac
     1261 tgtcgttgaa tgacttgctc ggtcgcgacc cgactgcgcc gctcacgctc gccgaggagg
     1321 ctgcgccctc cactccgccc gcggatacga tcgcggaagc ctggtcggga acaccggaag
     1381 tgcgacaggc agaagcgcag gtcgcggcgg cccgatcggg cacatcgctc gccgccgccg
     1441 agcggcggcc tcagctcgac ctcgtggtca acgcggggac gcaacccgcg ttcgagacct
     1501 cgggcggcgc cttcaacaac ggacagggat cgggcgtgga gtttctgctt tccttttcct
     1561 ggcccttgct cgatgccggt gggtaccgcg ctcggcgtga ccaggcgcag ctgcgcctgg
     1621 cggaagccca ggcgggggag atcgcggtgc gccgccaggc tcggctggtc tgggccgcgg
     1681 cgcgggcgga gcttgccgca cgttacgggg aggtcgagac acgccggcgg acggccggca
     1741 ccgcattgga ttcctatctc tcctccgaga gcctctatcg cgggggtgct gcgacggcgt
     1801 tggaggtgct cgacgcctat gggacctgga tcgccgcgag cgaggccgtg gcgggggcgg
     1861 tgcggtccta ccgcgtggcg gcggcgcagc tcgagcgttg ggggcagcca tgacgcgatg
     1921 gatcgtggtg ctggtgctgc tggccggctg cagcggaggg gatcagcagc gggagtccgc
     1981 ggcagcgtcc cgagacacct cgcccgaggg ggccgcgacg ccggtgagga ctgccgccat
     2041 ccggctcgcc acgctggctc tcaccatcag ggcacccggc cacaccgacg tattgcggcc
     2101 gttgcgcgtg cgggccccgt tcagcggcac cgtggcgacg ctccacgtgg ccgacggaga
     2161 ccgcgtcgcc cgtggggcaa cgctcgggac gttcgtgtcc cgcaacagcg ccgcggcgct
     2221 cgacggtgcg cgcgcgatgc tcgccgccgc ccggaccgaa gcggaacgac aggatgcaca
     2281 ccgcgcggtc gatctcgcga cgcgaggcct ggtcgaggtt ccgctgcacg cgtccgaagc
     2341 cggggtcgtc gtgtcgcacg cggccaatgc gggcgatctg gtaaccgagg gcgacgacat
     2401 tctcgtgatt gcgcccgcgg gcgccgtggc gttcatcgcc caggtcgtac agaccgactt
     2461 gccacgagtg cagcctgggc agcgggtcgc ggtggacctg gcggcgcgag ctcagcccct
     2521 ggcgggaacg gtgcatggga ttcttcccgc cgcctcggcg gagaacctca gcgccccggt
     2581 tcggatcgat ctcagcgccg gcggcgcagt cgggacgggg ctgttcggga cggcgcggat
     2641 cacggtggga gagcgacgac aggtgcccgt ggttccggat gcggcgttgc tgcaggacga
     2701 cgtgtacggc accacgcagg tcgcgatcgt cggcccggca ccggatcacc gcgcccactg
     2761 ggtgagtgtg aggccggggg tacgggatgg ggggctcgtc gagattctcg cgccgtcgct
     2821 ccctccgggc acgaccgtga tcgtgagtgg acaggtggga ctcccggagg gagccccggt
     2881 ccgcgccgag ccgtgagcct gatcgactcc gtacgccggc ggcctgccgc tctctatggc
     2941 gtcctgctgc tgctgacgat cggcggcgtg tacgcggcgg cgcgccttcc cgcctcgatc
     3001 ttcccctcgg tcacgttccc catcgtcaag gtgatcgccg acgccggtga agagccggcg
     3061 gcccggatga tgccgacggt gacccggccg ctcgaagaag cgatcctgcg cgtccccggc
     3121 gtgcgcctgg tccggtcgac gacgtcgcgg gggtcgagcg agatcagcgc cgagttcgcc
     3181 tggggcaccg acatgcaggt cgcgctgcag cgcgtccagg cggaaacgca gcgcatccgg
     3241 ccggatctgc cggcggagac gcggatcgac gtggagtgga tgaacacggc gatcttcccg
     3301 atccagggct atgccctcac ctcgtccacc cggtcgcagg ccgagctgct ggcgctcgcc
     3361 gagttcacgc tcaaaccggc cctgatccgg attcccggcg tttccgaggt acagattcaa
     3421 gggggtcggc gacgcgagtt cgaagtctgg ctggaccccg ggcagctcgc ggcccggcga
     3481 ctggcgccgg cggacgtggt ggaaacggtg cgccggagtc acgacgtgtt gtccgcgggg
     3541 ctggccgagc agaaccacga gctctacctc accctggtgg acggccgggt ggaggatctc
     3601 gcgggcctga gtcgcattgc cgtgccggtc gagtcggggc cgccggccac gctcggcgag
     3661 ctgggcgaga tcaaggtggc ggacgaggtg tcctacatcc gcacgacggc ggacggcgag
     3721 ccggccgtcc tgctgaatct gatccgtcag ccgtccgcga ataccgtcgc catcgccgct
     3781 ggcgtggagc agctgttccg cgagcagcca cagctgctgc cgtccgacgt gcgctggacc
     3841 acgttctacg accaggcgcg cttcgtgacg gattcggtgc atgggacgcg ggatgcgatt
     3901 ctgatcggca tcgtgctcgc cgcattggtg ctgctcgtgt ttctgcggag cctccgcctc
     3961 gcgctcgtgg ccgtcgcggc gattccgctg acggtggcga tcgtcggtct cgcgctggga
     4021 atcgcgggcc agaccgtcaa cctgatgacc ctggcgggcg tcgcggcggc aatcgggctc
     4081 gtcgcggacg acgccatcgt cgtcgtcgag cacatcgagc gccaccgcgc cgctgcgcgg
     4141 ggactgaccg atctcctccc ggcgatcgtc ggatcgagcc tctccaccac ggtgatcctg
     4201 ctgccgttcg cgctgctggc gggcatcgtc ggcgcgttct tcaagcccct cgcgctgacg
     4261 atggccctgg ccttgggcac gtcgttcctc gtcgccgcgc tggtcgtccc cgtgaccatc
     4321 ggcctggtcg gcgccgggaa ggaacgggct ggggccgccg cggctcccga acgcgcttcc
     4381 cgcttcctgt ccggctacga gcgactcgtg gcggcgtgta ttcggcacgg ccggctcact
     4441 gcggccgcgc tgggtgggtg tgtcgtggcc atgatattgc tctatcgcgg tgtcggtacc
     4501 gactttctgc ccgagatgga cgagggggcg atcatcctcg actattggac gcctcccggc
     4561 acgtcgctga ccgacaccga tgcgatgctc gacgaggccg aaaaggtcat ccgggcgctg
     4621 cccgacgtca cgagttactc acggcgcacc gggacccagc tggggttctt catcaccgag
     4681 ccgaaccgtg gcgactatgt catcaccttg aagccgcgtg gcgagcgacg cgacatcgag
     4741 acggtcatgg acgagctgcg ggaccgcatc caggccgtag agcccgcgat ccgcaccgat
     4801 ttcggccagc tgctggagga caacatcggc gacctcaccg gcggcgtgcc acagccgatc
     4861 gacatcaaga tcttcgggac cgaccccgcc cagctgcagg tgaaggcgcg ccagatcgcc
     4921 cggatcctgg acagcgtgcc cggcatggaa gacaccttcg acggcatcgt catcgccgga
     4981 cccgcgctga acatccgggt cgatgcgccg gcagcggcac gctacgggct ctccaccgcg
     5041 gacatccacg ctgccgtcga gcccgccgtc acgggcaccg tggtggacca gatccgggtc
     5101 ggcgaccgca tgtacgacct gcgcgtcttc atgcgacacg tgggggtcct gggcgacctg
     5161 ccggtgcggg ctgcctcggc gcccgcggcc ctgattccct tgtccaccat cgcgaccgtc
     5221 ggcaccggcg cccccgaagc cgagatcgac cgggagaatc tcaaaacgtt catcggcgtc
     5281 acggcgcggc tgtcgggtcg cgacctgggg ggcgccgtgg cggatatcca gcgtcggatc
     5341 gcacggaacg tccggctcgc gccggacatg tccatccagt acggcggcca gtaccagcag
     5401 caggaagatt ccttccgggg attgttctac gtgttgctcg gcagcctggt gctcgtctcg
     5461 ttggtcctgc tgttcgagtt cggcgactgg cgcgcgccgg tggtgacgtc ggcctgcgcg
     5521 gtcgcggtcc tcgcgggcgt gctgctcgcc ctcgtgctca ccggacagac cctgaacatc
     5581 tcgagctacg tcggggccat catgatggtc gggatcgtgg gggagaacgc gatcttcgtg
     5641 attcacgaag ggcggctcga gctggggcgt gggacggctc ccgcacaagc ctgggcccgc
     5701 gtgtcccgcc agcggctgcg gccggtcgcg atgacgatcc tcgccacggc gttcgcgctg
     5761 acgccgctcg ccgtcgcgct gggccacggc tctcagctga tgcagccgct ggcgatcgcg
     5821 gtgatcggcg gtttcgtcct gtccgggccc gtcgtcctga tcgtgttgcc gtccctgtac
     5881 tgcctcctcg atcccggggg acgacttggg tggggcggga ctcaattcca atgagacaaa
     5941 cggggaaccc gcccaggtgt gcatcccata cgaccgcggt ctcggctgcg cgcatgcccg
     6001 cccagcgctc caatggagga tggtcctggt cgatggcggc catcct
//
LOCUS       DBNGZW010000635         2457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1628888, whole genome shotgun sequence.
ACCESSION   DBNGZW010000635 DBNGZW010000000
VERSION     DBNGZW010000635.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1628888"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="AC16JC_14855"
     CDS             complement(<1..938)
                     /locus_tag="AC16JC_14855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HIR5166028.1"
                     /translation="MLGVDPEGAELFVRTVKREVLGLDLESGRVDTVATDVQRATLGP
                     DGTVYAVDAKRRVTTWSRRVRLTWPHPLTSDPTDLFGASNERLVATVGGDQPALVIAA
                     ADQPQASRPLSSDGDVEASRWGDLVAVASDSGVTLVDPLGRREPGFVSIADHPRALVF
                     SPSGHRIYVGSRSGLGLAVIDRYSLKEIDGVALPTPAAALRLDPFGQWLLARPSVGDT
                     VWVVDLPIKQLIGGLPTPWLADLPTVGPDGSLLVRRGNDVVAVRPDSLLETGRVSDGA
                     TDLWVATSWAPRGAAATTAAGSAAAADSAGTEGPLYV"
     gene            complement(1252..>2457)
                     /locus_tag="AC16JC_14860"
     CDS             complement(1252..>2457)
                     /locus_tag="AC16JC_14860"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR5166029.1"
                     /translation="VRPYHVRCSPDYTARPTIGYARDNFGRGFFGGAAVSLSDMLGDQ
                     TLQFAGAVNGRISEAQVFAAYVNWRHRLHWGVGGSQSPYYFYLPSTVSIVDVGNPGGS
                     DSALIYSLRIQRYVVRDVFAESYYPLSRFSRVELGLHGVSLGVATLTLSDGYDLQTGQ
                     YLGSLEADSNQASLGYVQPNIALVHDNALFGYVGPFAGSRSRFGISPAIGNKRFNTIT
                     ADYRRYLFFRPFTLAVRGLFYGNTGRDAGLFPIFLGSTELIRGYTSGSIINNECRASG
                     FVNTVGFTGCTELDQLIGTRVAVGNIEFRFPLTRSLVLGFLPVGLPPVEGALFYDAGV
                     AWEAGNKVKWNATAADDPLVVRSVLRSYGGSIRLNFLGFIILRFDVAKPLDRPHNKAY
                     WTISLGPTF"
BASE COUNT          429 a          805 c          886 g          337 t
ORIGIN      
        1 acgtagagcg ggccttccgt ccccgccgag tcggccgcag ccgcgctccc cgccgctgtc
       61 gtcgcggcgg cgccgcgagg cgcccaactg gtcgcaaccc agaggtccgt cgcgccgtcg
      121 gagacgcggc cggtttccag cagcgaatcg gggcgcacgg ccacgacgtc gttcccccgg
      181 cgcaccagca gcgagccgtc gggccccacg gtcggcaaat cggcgagcca gggagtgggc
      241 agccccccga tcaactgctt gatgggcaga tcgaccaccc acaccgtgtc gcccaccgaa
      301 ggtcgcgcga gcagccactg accgaaggga tcgaggcgga gcgcggccgc cggcgtgggg
      361 agtgccacgc cgtcgatttc cttcaggctg taccgatcga tcaccgcgag tcccagtcca
      421 ctgcgactgc ccacgtatat ccggtgaccc gacggcgaga acaccagcgc ccgcgggtgg
      481 tcggcgatcg agacgaaccc cggctcgcgc cggcccaacg ggtcgaccag cgtcaccccc
      541 gagtcgctcg ccacggcgac caaatcgccc caccggctcg cctccacgtc gccatcggag
      601 gacaacggtc gcgacgcctg gggctggtcg gccgcggcga tcaccagcgc aggctggtcg
      661 ccaccgaccg tcgcgacgag acgctcgttg gaggccccga agaggtcggt gggatcgctc
      721 gtcaacggat gtggccaggt gaggcggacg cgccgcgacc aggtggtgac ccggcgcttc
      781 gcgtccacgg cgtacacggt tccgtcgggc ccgagggtgg cgcgctggac atcggtcgcc
      841 acggtgtcga ctcggccgct ttccaggtcg agccccagga cctcgcgctt gaccgtgcgg
      901 acgaacagct cggcgccctc cggatctact ccgagcacgc gctcgaccgc cgggagcttg
      961 ccgcgtaacg gcgcaggcca gggggcgagg gtcgggaggc gatagagctg ggccgtcccg
     1021 ccgcttgcgg cgacccggat ggcgaatccg acccgccgcc ccggggggcc gatttcaggt
     1081 ctaagtgaag gcgctgcagc tgattgcgac gacgatccac ccggcgtctg ggtgcgacag
     1141 gcacacagcg ccgccagcgt caggagggga aagagtcgag gtggcagcaa atggcctcgc
     1201 cgcaagatag gaatgcggcg aggccaaggc aagtggtgca gccgtgcgga gttagaacgt
     1261 cggcccgagg ctgatggtcc agtacgcctt gttgtggggg cgatccagcg gcttcgcgac
     1321 atcgaagcgc aggatgatga atcccaggaa attgaggcgg atcgagccgc cgtaactgcg
     1381 cagcaccgac cggacgacca gggggtcatc ggccgcggtg gcgttccact tcaccttgtt
     1441 gcccgcctcc caggcgacac cggcgtcgta gaacagggcg ccttcgaccg ggggaagacc
     1501 gacggggagg aagccgagca cgaggctgcg ggtgagcggg aagcggaact cgatgttgcc
     1561 gaccgcgacc ctggtgccga tcagctgatc gagctcggtg cagccggtga acccgacggt
     1621 gttcacgaag ccgctggcgc ggcattcgtt gttgatgatc gaccccgagg tatagccgcg
     1681 aatcagctcg gtgctgccga ggaagatcgg gaacagaccg gcatcgcgcc ccgtgttgcc
     1741 gtagaagagg ccgcggaccg ccagggtgaa cggccggaag aacagatagc ggcgataatc
     1801 ggcggtgatg gtgttgaacc gcttgttgcc gatcgcgggg gagatgccga agcgcgagcg
     1861 gctgccggcg aacggcccca cgtagccgaa gagggcgttg tcgtgcacca gggcgatgtt
     1921 cggctgcaca tagccgaggg aggcctgatt gctgtcggcc tcgagggagc cgaggtattg
     1981 gcccgtctgc agatcgtatc catccgacag cgtcagggtg gcgacgccca ggctcacgcc
     2041 atggaggccg agctcgactc ggctgaagcg gctgagcgga tagtaggact cggcgaacac
     2101 gtcgcgtacg acgtaccgct ggatgcgcag cgagtagatg agggcgctgt ccgagccgcc
     2161 gggattgccc acatccacga tgctgaccgt gctgggcagg tagaagtagt acggcgattg
     2221 tgatcctccc accccccagt gcaggcgatg gcgccaattg acgtacgccg cgaagacctg
     2281 cgcctcactg atccggccgt tcaccgctcc ggcgaactgc agcgtctggt cgccgagcat
     2341 gtcggaaagc gagaccgccg cgccgccgaa gaacccgcgg ccgaagttgt cgcgggcgta
     2401 gccgatcgtc ggccgggcgg tgtagtcggg gctgcaccgc acgtgatagg ggcgaac
//
LOCUS       DBNGZW010000636         5731 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_331330, whole genome shotgun sequence.
ACCESSION   DBNGZW010000636 DBNGZW010000000
VERSION     DBNGZW010000636.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5731
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_331330"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="AC16JC_14865"
     CDS             complement(<1..782)
                     /locus_tag="AC16JC_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR5166030.1"
                     /translation="MEQTYFRKGFGLKDAIEGALTTDYHSRLVDAIRANGHTLVLGDL
                     TFRLAREFGFCYGVDRAVEYAYEARSKFPERRAFLVGEIIHNPHVNSKLRAMGVQFLF
                     RGPGGDFDFSAVTQSDVVILPAFGVTVQDFARLREIGCVLVDTTCGSVLNVWKRVESY
                     ARDGYTAVIHGKYFHEETKATASQVTKYPGGKYIIVRHMEEAREVCAFLEGTGKRAQL
                     LERFAHAVSPGFDPDRDLVKIGVANQTTMLSNESLAIAGELQA"
     gene            complement(787..1851)
                     /locus_tag="AC16JC_14870"
     CDS             complement(787..1851)
                     /locus_tag="AC16JC_14870"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5166031.1"
                     /translation="MTVRVGMIGTGFARRVQLPGLRLVPGVQVTAIASGRRANAEAVA
                     EEFGIARVYDDGAELARASDVDLVIISSTPPGHARFALAALEAGKHVLCEKPMALDAA
                     EAERMALAAERKPSQVAWIDHELRYEPNRRKLRALVRAGAIGEVRHIELSLKPYLRGD
                     GRPQTASAPWTWWFDVTQGGGLLGAVGSHLIDLCRFWTGTEAVGVAGGVRTFIGERSD
                     AQDARHEVTADEFVSGVLTLGGGAVATLTLSAVAHHGPGHLAQVTGRDGSLVLTGEGR
                     LEIGRSGAALEDITVADDLAGRTSVNNMWARSFVRLARDLVSVIGGSQPEGEPAVFRD
                     GWRVQHVLDALRTGRPTPLD"
     gene            complement(1848..2357)
                     /locus_tag="AC16JC_14875"
     CDS             complement(1848..2357)
                     /locus_tag="AC16JC_14875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166032.1"
                     /translation="MRRRAGACLAVALAACGKPAEPHGTGRVRLALQGSTDTVRFEVP
                     VIARRCAGGGGVLVTGAREGQGVLLLLRSAEPAIDTGAYVLLTRADSAALQGAIVAIR
                     FSVGPTSHGLTVDDGTARVSQTTPTLAADVRGRGMEAGTGGGAQRTAAFTVDDVPLAP
                     DSEFCRVQP"
     gene            complement(2354..2824)
                     /locus_tag="AC16JC_14880"
     CDS             complement(2354..2824)
                     /locus_tag="AC16JC_14880"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR5166033.1"
                     /translation="MPSVAPSPYIIEEYVRWSDVDFAGIIFYGSYVRFFEIAETELFR
                     ACGLAYKDVFDRYDIFLPRKAVHSEFFHPPRLDDRLRVATYVGRVGTTSMTLNFDVLR
                     AGAPALAAAGWMVLVCVDRERLRPRPLPPALIDALAPHTLSLAAARAALGVAEP"
     gene            complement(2847..3245)
                     /locus_tag="AC16JC_14885"
     CDS             complement(2847..3245)
                     /locus_tag="AC16JC_14885"
                     /inference="COORDINATES: protein motif:HMM:NF014950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5166034.1"
                     /translation="MDALVRAVLESLEQGIAVFDVGGGLVYANTAARRLLAASKGSAL
                     EARRALLARQGRSVPLRDGETTLGEAVLVGGDGGTVTLAEQERQAILDALALTGGKLA
                     EAARRLGISRTTLWRRLRSYGPAERPGAAS"
     gene            complement(3280..5730)
                     /locus_tag="AC16JC_14890"
     CDS             complement(3280..5730)
                     /locus_tag="AC16JC_14890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011552226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR5166035.1"
                     /translation="MELAMGLHIDTLLEREHGEDVRRLAVSGRGFGIMAGTGTGKTLA
                     IRPIAERILGAPLRVGVVNREREATPETPTWNVVIVTTGIARRWFEDGLITARDTLVV
                     DEIHQTSAELELCLALGKRARCRYMWLSATVDPTFYARYLDSAEVLETTAFDPARAAR
                     VRVVPVQPLDFLDERFVRRVLKERRGVAVFVPTRAEVEELAGRLGAQWPRLTAAFYHG
                     GEPIRVIRPFLDGEVERPFLLAMTAAGQSALNIPGLDTVVIYDARYANVVNRGRNVLT
                     RLYLGANEILQMAGRVHGRVSNGEVYILSDRNLEFAALRPTPPEFQLAGDAERVAITC
                     AAMGVDAADLDLPVPLDRHAYRDAVRLLSERGLVEEGRLTRYGRAVESMPVERPWGEL
                     LVHAEADLVPIVAVSSNIDSLHRMTRDPRNLKGLIVPGSDHLTAYNIFAEAVNKHGYL
                     GEVYGLPRHLFRETLERWAEWRGALIKALEDIALGTASVYRQLERPLPERLPVAQERE
                     LAAFQDLVARIQPFDLVIDEETADGREARVSRGSVCGSWGAIAGNLRYFADRFGNPRA
                     GIEGTQVPERLVRRYARRGAATVEFVEARGGGGRGRLIVVRTVEYFGFLLEREEELLA
                     SPFPAALAEAARAAIAAALAGGRTPHPNQRAVRQALDRAGEYWRRSGGSLGAVAPEHL
                     TSRLIEQLSGVDSWDGFIRRPLALDVGALVPAEVRERLDALPSSVHLYGDRVPLDYEM
                     EPGGGVVRLRLKEGQARRLRAEDLPELDRPLRFSVLRGRRPAVRADSLEDMRRQLTEL
                     PRTERARLVRGGRRPRRR"
BASE COUNT          898 a         2133 c         1907 g          793 t
ORIGIN      
        1 gcctgcaact ccccggcgat cgccagcgat tcgttggaga gcatcgtcgt ctggttggcc
       61 acgccgatct tcacgaggtc gcggtccggg tcgaaaccgg gcgagacggc atgcgcgaag
      121 cgctcgagca gctgcgcgcg cttcccggtc ccctcgagga acgcgcacac ctcgcgggcc
      181 tcctccatgt gccggacgat gatgtacttc cccccgggat acttggtcac ctggctggcg
      241 gtggccttcg tctcctcgtg gaagtatttg ccgtggatca ccgccgtgta gccgtcgcgc
      301 gcgtaggact cgacgcgctt ccagacgttc aacaccgagc cgcacgtcgt gtcgaccagc
      361 acgcagccga tctcccgcaa ccgggcgaag tcctgaaccg tgactccgaa ggccggcagg
      421 atgaccacgt cggactgcgt caccgcgctg aaatcgaaat cccctccggg accacgaaac
      481 aggaactgca ctcccatggc gcgcagcttg gagttgacgt gcgggttgtg gatgatctcg
      541 cccaccagga aagcgcgccg ctcgggaaac ttgctgcggg cctcgtacgc gtattcaacc
      601 gcccggtcca ccccgtagca gaacccgaac tcccgggcca ggcggaatgt cagatccccc
      661 aggacgaggg tgtggccgtt ggcgcggatg gcgtccacga gccggctgtg gtagtcggtc
      721 gtgagcgcgc cctcgatggc gtccttgagc ccgaagccct tgcggaagta cgtctgctcc
      781 acgaggtcaa tctagcggcg tgggcctacc ggtgcgcagc gcatcgagca cgtgctgcac
      841 gcgccagccg tcgcggaaga cggccggctc gccttcgggc tggctgccgc cgatcacgga
      901 cacgagatcg cgtgcgagcc ggacgaaaga gcgggcccac atgttgttga ccgaggtccg
      961 gccggcgagg tcgtcggcca cagtgatgtc ctcgagcgcc gctcccgagc ggccgatctc
     1021 cagccgcccc tctccggtga gcacgagcga gccgtcccgc ccggtcacct gggccaggtg
     1081 gccgggcccg tgatgcgcga cggcggagag tgtgagcgtc gcgaccgctc ctccgcccag
     1141 ggtcaggacc ccggacacga attcgtcggc ggtgacttcg tgccgggcgt cctgcgcatc
     1201 ggaccgctcg ccaatgaacg tccggactcc cccggcgacg cccaccgcct cggtgcccgt
     1261 ccaaaaccgg cacaggtcga tcaggtgcga gcccaccgcg ccgagcaggc cgccaccctg
     1321 ggtcacgtcg aaccaccagg tccacggcgc gctcgcggtc tgcggccggc cgtcaccccg
     1381 caggtagggt ttgagcgaca gctcgatgtg gcgaacctcg ccgatggcgc cggcacgcac
     1441 cagcgcgcgg agcttgcgac ggttcggctc gtagcgcagc tcgtgatcga tccacgccac
     1501 ctgcgagggc ttccgctccg ccgcgagcgc catgcgctcc gcctcggccg cgtcgagcgc
     1561 catcggcttc tcgcacagca cgtgcttccc ggcctcgagc gccgcgaggg cgaagcgcgc
     1621 gtgaccggga ggagtagaag aaatgatcac gagatccacg tcgctcgcgc gcgccagctc
     1681 ggccccgtcg tcgtagacgc gcgcgatgcc gaactcctcg gccaccgcct ccgcgttggc
     1741 gcgccgcccg ctcgcgatcg ccgtcacctg cacgccgggc accagacgca gtccgggcag
     1801 ctgcacccgc cgcgcgaacc cggtgccgat catccccacg cgcaccgtca tggctggacg
     1861 cggcagaatt cggaatcggg ggcgagcggg acgtcgtcca ccgtgaaagc cgccgtgcgt
     1921 tgcgctcccc cccccgttcc cgcttccatt ccacgtccgc gcacgtcggc ggccagcgtc
     1981 ggcgtcgtct gcgatactcg ggccgtcccg tcgtccaccg tcaggccgtg agacgtcgga
     2041 ccgaccgaga atcgaatggc cacgatcgcc ccctgcaggg cggcgctgtc ggcccgggtc
     2101 aacagcacgt acgccccggt gtcgatcgca ggctccgcag accggagcaa cagcagcacg
     2161 ccctgaccct cccgggcccc ggtcaccaga acgccgccac cgccggcaca gcgccgcgcg
     2221 atgacgggga cctcgaaccg gaccgtgtcc gtcgatccct gaagcgcgag acgcacccgc
     2281 ccggtcccgt gcggctcggc gggtttgccg cacgccgcga gcgcgacagc gaggcaggca
     2341 ccggcgcggc gcctcatggc tccgccacgc cgagcgcggc ccgcgcggcg gccaggctga
     2401 gcgtatgagg ggcgagcgca tcgatcaggg ctggcgggag cgggcgtgga cggaggcgct
     2461 cgcgatcgac gcagacgagc accatccagc cggcggccgc gagggcgggc gcaccggcgc
     2521 gcagcacgtc gaagttgagg gtcatcgagg tcgtgccgac ccgcccgacg tacgtcgcca
     2581 cccgcagccg gtcatccagc cgggggggat ggaagaactc actgtgcacc gctttccgag
     2641 gcaggaagat gtcgtagcgg tcgaacacgt ccttgtacgc gaggccgcag gcacggaaca
     2701 gctccgtttc ggcgatctcg aagaaccgca cgtaggagcc gtaaaagatg atccccgcga
     2761 agtccacgtc ggaccagcgg acgtactcct cgatgatgta gggactgggg gcgacggagg
     2821 gcatcgcccc gggaagctag aaagggctag gaagctgcgc caggacgctc ggccgggccg
     2881 taagaccgca ggcgccgcca caatgtggtg cggctgatgc ccaggcgtcg cgcggcttcc
     2941 gccaacttgc ctcccgtgag ggccagggcg tcgagaatcg cctgacgctc ctgctcggcc
     3001 agcgtcacgg tgccgccgtc cccgccgacc agcaccgcct cgcccagagt ggtttcaccg
     3061 tcccgcaacg ggacgcttcg accctgtcga gccaacaggg cgcgccgcgc ttccagggcg
     3121 ctccccttcg aggcggcaag caagcgccgg gcggcggtat tggcgtacac caatccgccg
     3181 ccgacgtcga acaccgcgat cccctgctcg agcgattcga gtaccgcgcg aacgagcgcg
     3241 tccatcggca ccccttacca tcggttccac cgaaatgttt catcgccggc ggggacgccg
     3301 ccccccgcgc accagccgag cccgctcggt ccgggggagc tccgtgagct gccggcgcat
     3361 gtcttccagc gagtccgccc gcaccgccgg ccgccggccg cgcagcacgc tgaagcgcag
     3421 gggccgatcc agctccggca ggtcctcggc ccgcagccgg cgcgcctgcc cctccttgag
     3481 acgcagccgc acgacccccc cacccggctc catctcgtaa tcgagcggga cgcggtcacc
     3541 atacaggtgg accgaggacg gcagggcgtc cagtcgctcg cgcacctccg ccggaacgag
     3601 cgcgcccacg tcgagcgcca gaggccgacg gatgaacccg tcccagctat ctacccccga
     3661 cagctgctcg atgagccgtg acgtgaggtg ctcgggggcg accgcgccca ggctgccgcc
     3721 ggatcgccgc cagtattcgc cggcgcgatc gagagcctgc cggaccgccc gctggttggg
     3781 atggggggtc cggcccccgg cgagcgcagc ggcgatcgcc gcccgggccg cctcggccag
     3841 cgcggccgga aacggtgagg ccaggagctc ttcttcccgc tccagcagga acccgaagta
     3901 ctccacggtg cggacgacga tgagccgtcc ccgccctcct ccccctcgcg cctccacgaa
     3961 ctccaccgtc gccgcgccgc gccgcgcgta gcgacgcacc agccgctcgg ggacctgggt
     4021 cccctcgatg ccggcgcgcg gattcccgaa gcgatcggcg aagtagcgca gattgcccgc
     4081 gatggccccc caactgccgc acaccgagcc gcgcgacacg cgggcctcgc ggccgtcggc
     4141 cgtctcctcg tcgatcacca ggtcgaacgg ctggatgcgg gccacgaggt cctggaaggc
     4201 cgccagctcc ctttcctgag cgacggggag ccgttccggc agcggccgtt ccagctggcg
     4261 gtacaccgag gcggtgccca gggcgatgtc ctcgagcgcc ttgatgaggg ccccacgcca
     4321 ttcggcccat cgctccagtg tctcacggaa caggtggcgc ggcaggccgt acacttcgcc
     4381 caggtaaccg tgcttgttga ccgcctccgc gaagatgttg taggccgtga ggtggtcgct
     4441 accggggacg atcagtccct tcaggttcct cgggtcgcgg gtcatccggt gcagcgaatc
     4501 gatgttcgac gatacggcga cgatcggcac gagatccgcc tccgcgtgca cgaggagctc
     4561 gccccaggga cgctccaccg gcatcgattc gaccgcacgc ccgtagcgcg tgagccgccc
     4621 ttcttcgacg agcccgcgct cgctgagcag tcgcaccgcg tcgcgatacg cgtgacggtc
     4681 cagtggcacc gggaggtcca ggtccgccgc atccaccccc atcgccgcgc aggtgatcgc
     4741 gacacgctca gcatcgcccg cgagctggaa ctccggtgga gtggggcgca aggcggcgaa
     4801 ctcgagattg cggtcgctca ggatgtacac ctcgccgttc gacacccgcc cgtggacgcg
     4861 ccccgccatc tggaggatct cgttcgcacc cagatagagg cgcgtgagca cgttgcgacc
     4921 ccgattcacc acgttcgcgt agcgggcatc gtagatcacc accgtgtcga gccccgggat
     4981 gttcagcgcc gattgacccg cggcggtcat cgcgagcagg aacggccgct ccacttcgcc
     5041 gtccaggaag gggcggatga ctcgaatcgg ctcgcctccg tggtagaagg ccgccgtgag
     5101 ccgcggccat tgcgccccca gccggccggc gagctcctcc acctccgcgc gggtgggaac
     5161 gaagaccgct acgccgcgtc gctccttcag gacccgccgc acgaatcgct cgtccaggaa
     5221 gtcgagcggc tgcaccggca cgacccgcac ccgtgccgca cgcgccggat cgaacgcagt
     5281 ggtctccagc acctcggccg aatcgagata gcgcgcgtag aaggtcggat ccaccgtcgc
     5341 cgacaaccac atgtagcggc accgtgcacg cttgccgagc gccagacaca gctccagctc
     5401 ggcggaggtc tgatggatct cgtccacgac cagcgtgtcg cgcgccgtga tcagtccgtc
     5461 ttcgaaccag cgacgcgcga tcccggtcgt cacgatgacc acgttccacg tcggcgtctc
     5521 cggtgtcgct tcccgctcgc ggttcaccac gccgacccgg agcggggcgc cgaggatgcg
     5581 ctcggcgatc ggccggatgg cgagcgtctt cccggtgccg gtgccggcca tgatcccgaa
     5641 tccgcgcccg gacacggcca ggcggcgcac atcttccccg tgctcgcgct ccagcagcgt
     5701 gtcgatatgc agtcccatcg cgagctcgat g
//
LOCUS       DBNGZW010000637         6237 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_726934, whole genome shotgun sequence.
ACCESSION   DBNGZW010000637 DBNGZW010000000
VERSION     DBNGZW010000637.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6237)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6237)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6237
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_726934"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..1635
                     /locus_tag="AC16JC_14895"
     CDS             4..1635
                     /locus_tag="AC16JC_14895"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014395663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR5166036.1"
                     /translation="MPYLSRREFIEYAGLGTIAGVVRPAAGSATPRVAAPPVIAPAPF
                     ELEEVTIAQLQEGLRSGKYTSRRLCQLYLARIDEIDRRGALRSIIETNPDALALADAA
                     DAERRAGRVRGPLHGLPVVIKDNIATGDKMKTTAGSLALAERPVPHDATVARRLRDAG
                     AILLGKTNLSEWANFRSTHSVSGWSGRGGLCRNPYALDRNPCGSSSGTGAAISANLAA
                     VGVGTETDGSIVCPSSANSLVGIKPTLGLVSRRGIIPIAHSQDTAGPMARTVADAAAL
                     LGVLAGTDPEDPATAAAHVERDYTKFLDRDGLRGARIGVARTKFFGYSANADKLVEAA
                     IDVMKRAGAVIVDPADIPHAGEYDDAEFTVLLYEFKADLNGYLAGLGDQASNRTLADL
                     IAFNDKAGERELPHFGQEIFTMAQEKGPLGDDAYVEALAHCRRLSRTEGLDVVFAKEK
                     LDAIVTPTGSPPWTTDLVNGDHFLGASSTPAAVSGYPSVTVPVGYVHGLPVGMSFIGT
                     AWSEPILIKLAYAYEQAVQPRRPPRFLSTAELGGA"
     gene            1728..4688
                     /locus_tag="AC16JC_14900"
     CDS             1728..4688
                     /locus_tag="AC16JC_14900"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HIR5166037.1"
                     /translation="MVKRAGLLMAVLVAFGMARVSAQERQITGVVTAAGGVPITGVSV
                     SVVGTTFSTVTHEDGRFVISAPTSAVTVLFRRIGYKRREAGVAADQSSVDVELEQDVF
                     NLEAVVVTGQQTGVERRNAAVATTVVTGAEVTAVPAPALDRALQGRVPGAYIQQNSGA
                     PGGGTQIQIRGSNTVIGAADPLYVVDGVIVSNASLSTGLFAITASGNPQSTRNDGEKQ
                     DDPVNRLTDLNPNDIETFQILRGAAATSIYGSKGVNGVVIITTKRGQSGKPRATITQR
                     VGFSELQRGPGTRSYTLDEALSQFGDTALIQSYLVNGVLPTYDHLRELAGNKPLSNET
                     QIDVRGGSSGTLYYLSGGLKGDGGIIENTGAQRQNLRANVDQSFSDRFTLGLTTAFDR
                     TTTQRGFTNNDNAGASVTYALAYIPGFVPLEPVNGVFPDPGGLSYQGANPLQTTALGS
                     NDETAVRFTGGLNLRYQALQSEHQSLQLVGAGGIDFFNQKDEVFAPPELYFEARQTNP
                     GGSVLSNADGRFANWNLNAIHTYTASTGSWTATSAAGIQWEDRAISRSRIIARGLLPG
                     QQNVNQGAVITPFEENTHERTAALYGQEEWLGVEQRLLLSASVRAERSSANGDQNKYY
                     LFPSFRGSYRFPDLLGAGSDVKLRAAYGETGNQPLFGQKFTTLQGGQVIGGNIGTTSG
                     AIAGDPTIRPERTREVEAGVDATLLSGRANLELTLFQRKTSDLLVPVTPAPTTGFSLA
                     FVNGGKIKNEGLEIGVGYRPVATPTFDWLLRTNFTSIRNRVLELNLPGGAQGFRPANA
                     GYGLAYGEFFVQVGRPITQIIGTDDQGNVISLGQVNPKFRWSLSNEFTYHRASLSFMW
                     EWQDGGVAQNQTLSLYDCNNLAPDGGTPAGQARNDACINTGDARPFVQSTSFLKLRNA
                     VFSLDLPDRFASWFGAKSAALSVEGVNLITISDYFGYDPEVSNYGAQAITRNIDLGPY
                     PPARQFFFSIRAGF"
     gene            4691..6148
                     /locus_tag="AC16JC_14905"
     CDS             4691..6148
                     /locus_tag="AC16JC_14905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HIR5166038.1"
                     /translation="MRTRSFAIAAALLVIGGCNFDVLNTNQPTQDDLVNNPTRAKLSA
                     AATGLFASARGGIQALIWRLGSMGREGVNLSGNNQPDFQEPYFGPVTAGGSFGGTLYI
                     DRYAHIRSANIYLAAVDKAPDLSAAEKAASRGMAKTLKALAFAYAIETRAQLGSAVEV
                     DLPVTSAPAPFVTEDSVWGYIVGLLNDAQADLTAAGSADFPFPVPPGLAAFNTPATFI
                     QFNRALAAKAYVFRATAQNGCGGNVATCYAGALTALWSSFLSTDPAAFQTGAYFDFSN
                     APGDATNGLSEPLNGLSFYALQDNLSDAQLQTGGVLKDRRVLDKIVPATDTNIGLLGS
                     IPIPGELKFAVFFTDGNADENHGIPIIKDEELVLLRAEAGWFGGNKAQAIADIDLVRQ
                     NSGGLPATTLTTGSTDQAFVTELMYNRRFSLLWEQGTRWIDARRFGRLTDIPVAVPAG
                     NVPPYMPIPLTECQARNLATGPATNDVVTCTPLNP"
BASE COUNT         1056 a         2078 c         2130 g          973 t
ORIGIN      
        1 gccatgccgt atctgtctcg tcgggagttc attgaatatg cagggctcgg gaccatcgcc
       61 ggcgtcgtgc ggcccgcggc gggcagcgcc accccgaggg tcgcggctcc tccagtcatc
      121 gcccccgcgc cgttcgagct cgaagaagtc acgattgcgc agctgcagga gggcctgagg
      181 agcgggaagt acacgtcccg gcggctgtgc cagctgtatc tggcccgtat cgacgaaatc
      241 gaccggcgcg gcgcgctgcg ctcgatcatc gagaccaatc ccgacgccct cgccctggcc
      301 gacgcggcgg atgccgagcg ccgggcgggc cgcgtgcgtg ggccgttgca cggcctcccc
      361 gtggtcatca aggacaacat cgccacgggc gacaagatga aaacgaccgc cggctcgctc
      421 gcgctcgccg agcgcccggt gccgcacgac gcgacggtgg cgcgccggct gcgcgacgcg
      481 ggcgcgattc tgctcggcaa aacgaacctc tcggaatggg ccaacttccg ctcgacgcac
      541 tcggtgagcg gatggagcgg gcgcggcggc ctgtgccgca atccttacgc cctggaccgc
      601 aacccctgcg gctcgagctc ggggaccggg gcggcgatct cggccaacct cgcggcggtg
      661 ggtgtgggga ccgagaccga cggctcgatc gtgtgtccgt cgtcggcgaa ttcactcgtg
      721 ggcatcaagc cgacgctcgg cctcgtgtcg cgccgcggca ttatcccgat cgcgcactcc
      781 caggacacgg ccggccccat ggcccgcacg gttgcggacg cggcggcgct gctcggtgtg
      841 ctggccggaa cggaccccga ggacccggcc accgccgcgg cgcatgtgga gcgtgactac
      901 acgaagttcc tcgaccgcga cggcctgcgg ggcgcgcgga tcggtgtggc gcgcaccaag
      961 ttcttcggct acagcgcgaa cgccgacaag ctggtcgagg cggccatcga cgtcatgaag
     1021 cgggccgggg cggtgatcgt cgatcccgcc gacatccccc acgccgggga atacgacgac
     1081 gccgagttca ccgtcctcct gtacgagttc aaggccgacc tgaacggcta cctcgccggg
     1141 ctcggcgacc aggcgtccaa ccggacgctg gccgatctga tcgcgttcaa cgacaaggcg
     1201 ggcgagcggg aattgcccca tttcggccag gagatcttca cgatggccca ggagaaggga
     1261 ccgctcggcg acgacgccta tgtcgaggcc ctggcccatt gccggcggct gtcgcggacc
     1321 gagggcctgg acgtcgtctt cgccaaggag aagctcgacg ccatcgtcac gccgacgggc
     1381 agtccgccct ggacgaccga tctcgtgaac ggggaccatt tcctgggggc gtcgtcgacg
     1441 ccggccgcgg tgtccgggta tccgagtgtg acggtgccgg tcggatacgt ccatgggctg
     1501 ccggtgggga tgtccttcat tggaacggcg tggagtgagc cgatcctcat caagctggcc
     1561 tacgcctacg agcaggcggt ccagccacga aggccgccgc ggttcctgtc caccgccgag
     1621 ctgggcggtg catgaacgtg aagatcacgg cttgcgaggc tggagattcg gcggcaacgt
     1681 tgcagtcctg ccgtcatgat ttccggatca cccaccctgg agggattgtg gtcaaacgtg
     1741 caggtctgct catggccgtg ctggtcgctt tcggcatggc ccgcgtttcg gcgcaggagc
     1801 gtcagatcac cggcgtcgtg accgccgccg gcggcgtccc catcacgggt gtctccgtgt
     1861 cggtcgtggg gacgacgttc agtaccgtca cgcacgaaga cggccgcttt gtgatctcgg
     1921 caccgacgag cgccgtaacg gtgctgttcc ggcgtatcgg ctacaagcgg agggaagccg
     1981 gcgtggcggc cgatcagtcg agcgtggacg tggagctcga gcaggacgtg ttcaatctgg
     2041 aggccgtcgt cgtcaccggc cagcagacgg gcgtggagcg gcgcaacgcg gccgtcgcca
     2101 cgacggtcgt gactggggcg gaggtgaccg cggtgccggc cccggcactg gaccgcgcgc
     2161 tgcagggacg cgtgccgggt gcctacatcc agcagaattc cggcgctccc ggcgggggca
     2221 cgcagattca gatccgcggc tcgaacacgg tgatcggcgc ggccgacccg ctgtacgtgg
     2281 tggacggagt gatcgtcagc aacgcgtcgc tgtcgacggg cctgttcgcg atcacggcga
     2341 gcggtaatcc ccagtccacg cgcaacgacg gggaaaagca ggacgatccc gtgaaccggc
     2401 tgaccgatct gaatcccaac gacatcgaga cctttcagat tctgcggggc gcggccgcga
     2461 cgtccattta cggatccaag ggcgtgaacg gtgtggtgat catcaccacg aagcggggcc
     2521 agtccggcaa gccgcgggcg acgatcacac agcgcgtcgg gttctcggag ctgcagcgtg
     2581 ggccgggaac gcggtcctac acgctggacg aggccctgag ccagttcggg gacacggcgc
     2641 tcatccagtc gtatctggtg aacggggtgc tgccgaccta cgatcacctg cgggagctgg
     2701 ctggcaacaa gccgctctcc aatgagacgc agatcgacgt gcggggcgga tcgtccggca
     2761 ccctgtacta tctgtcgggc ggcctgaagg gtgacggggg gatcatcgag aacacgggcg
     2821 cgcagcggca gaacctgcgg gccaatgtcg accagagctt ttccgatcga ttcacgctgg
     2881 ggctgacgac ggcgttcgac cgcacgacca cccagcgcgg cttcaccaac aacgacaacg
     2941 ccggggccag cgtcacttac gcgctggcgt acatcccggg attcgtgccc ctcgagccgg
     3001 tgaacggcgt ctttcccgac ccgggcggac tgagctacca gggggccaac ccgctgcaga
     3061 cgacggcgct ggggagcaac gacgaaaccg ccgtacgctt caccgggggg ctgaacctgc
     3121 ggtatcaggc actgcaatcg gagcaccaga gcctccagct ggtcggcgcc ggcgggatcg
     3181 atttcttcaa tcagaaggac gaagtgttcg ccccgccgga gctctatttc gaggcacggc
     3241 aaaccaaccc cgggggctcg gtgctgtcca atgcggacgg ccggttcgcg aactggaacc
     3301 tcaacgcgat ccatacctac accgcgagca ccggctcctg gacggccacc tcggcggccg
     3361 ggatccagtg ggaggaccgg gcgatctccc ggtcccgcat catcgcgcgc gggttgctcc
     3421 ccggccagca gaacgtgaac cagggagcgg tgatcacgcc gttcgaagag aatacgcacg
     3481 agcgcaccgc cgccctgtat gggcaggagg agtggctcgg agtggagcag cggctgctgc
     3541 tgtcggccag cgtgcgggcg gagcggtcca gcgccaacgg cgaccagaac aagtactatt
     3601 tgttcccctc gttccgggga tcgtaccggt tcccggatct gctgggcgcg ggcagcgacg
     3661 tgaagctgcg tgccgcctac ggtgaaacgg gcaaccagcc gctgttcggc cagaaattca
     3721 ccacgctgca gggcggacag gtgatcggcg gcaacatcgg gaccacgtcg ggggccatcg
     3781 ccggcgatcc caccatcaga ccggagcgga cgcgggaggt cgaggccggc gtggacgcga
     3841 cgctgttgtc gggtcgcgcc aacctcgagc tgacgctgtt ccagaggaag acgagcgatc
     3901 tgctcgtgcc cgtcacgccg gcgccgacga cgggcttctc gctggctttc gtcaacggcg
     3961 gcaagatcaa gaacgaggga ctggagatcg gcgtcggtta ccgtccggtg gccacgccga
     4021 cgttcgattg gctgttgcgt accaacttca ccagcatccg gaaccgggtg ctggagctga
     4081 acctcccggg cggcgcgcaa gggttccggc ccgccaacgc cgggtatgga ctggcctacg
     4141 gcgaattctt cgtgcaggtg gggcggccca tcacccagat catcggcacc gacgaccagg
     4201 ggaacgtgat ctccctgggg caggtgaacc ccaagttccg ctggtcgctc tccaacgagt
     4261 tcacctacca ccgggcctcc ctgtcgttca tgtgggagtg gcaggatggc ggggtcgcgc
     4321 agaatcagac cctgtcgctg tacgactgca acaacctcgc gcccgacggc ggcacgccgg
     4381 cgggtcaggc acgcaacgac gcctgcatca acaccggcga cgcacggccg ttcgtgcaat
     4441 ccacgtcgtt cctcaaactg cgcaacgccg tcttctcgct cgatctgccg gaccggttcg
     4501 cttcgtggtt tggggcgaag tcggcggcgt tgagtgtcga aggcgtgaac ctgatcacca
     4561 tcagcgacta cttcggctac gatcccgaag tctccaacta tggggcgcaa gcgatcacgc
     4621 ggaacatcga tctcggaccg tatccgccgg cgcgccagtt cttcttctcc atccgggcgg
     4681 ggttctgacc atgcgcacga gatctttcgc cattgcggcg gccctgctcg tgatcggggg
     4741 ctgcaatttc gacgtgctga acaccaacca gccgacgcag gacgatctgg tgaacaaccc
     4801 gacccgggcg aagttgtcgg ccgcggccac gggactgttc gccagcgcgc ggggcggcat
     4861 tcaggcgctc atctggcggc tggggtcgat gggtcgggag ggcgtcaacc tctccggcaa
     4921 caaccagccg gacttccagg agccgtattt cggcccggtc acggccggcg gcagcttcgg
     4981 cggcacgctc tacatcgacc gctatgccca tatccggagc gcgaacatct atctcgccgc
     5041 ggtggacaag gcgcccgatc tgtcggcggc ggagaaggcg gcgtccagag ggatggcgaa
     5101 gacgctcaag gcgctcgcct tcgcctacgc catcgagacc cgggcgcagc tcgggtcggc
     5161 cgtggaggtg gaccttccgg tcacgtccgc acccgcgccg ttcgtcacgg aggacagcgt
     5221 ctggggctac atcgtcgggc tgctgaacga cgcccaggcc gatctcacgg cggccgggag
     5281 cgccgacttc ccgttcccgg tgccgcccgg gctggcggcg ttcaacacgc cggccacgtt
     5341 cattcaattc aatcgcgcac tcgcggcgaa agcgtatgtg ttccgcgcca cggcccagaa
     5401 cgggtgcggt gggaacgtcg ccacgtgcta cgcgggggcg ctcacggcgc tctggtcctc
     5461 gttcctgagc acggacccgg cggcgtttca gaccggcgcc tatttcgatt tctcgaatgc
     5521 ccccggcgac gccacgaacg gcctgtcgga gccgttgaac ggcctctcgt tctacgccct
     5581 gcaggacaac ctgagcgacg cgcagctgca gacgggcggc gtgctcaagg accggcgcgt
     5641 gctggacaag atcgtgcccg ccaccgatac caacatcggg ttgctgggca gcattccgat
     5701 tcctggtgag ctcaagttcg cggtgttctt cacggacgga aatgccgacg agaaccacgg
     5761 cattccgatc atcaaggacg aggagctggt gctgctgcgc gccgaggccg gctggttcgg
     5821 cggcaacaag gcgcaggcga tcgccgacat cgatctggtg cggcagaatt ctggcggcct
     5881 gcccgcgacg acgctcacga ccggcagcac cgatcaagcg ttcgtcaccg aactgatgta
     5941 caaccggcgc ttctcgctcc tgtgggagca gggaacccgc tggatcgacg cccggcgctt
     6001 cgggcgattg accgatatcc ctgtggctgt cccggcggga aacgtgccgc cctacatgcc
     6061 gattccgctc acggaatgtc aggcgcgcaa cctcgcgacc gggccggcga ccaacgacgt
     6121 ggtgacctgc acgccgctga atccgtaggg aggatggggg aggttgggcc caccaacctc
     6181 ccccgacctc caccggcctc caccaacctg cttcaaccgt gttcaccccc gaacaac
//
LOCUS       DBNGZW010000638         7063 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_113783, whole genome shotgun sequence.
ACCESSION   DBNGZW010000638 DBNGZW010000000
VERSION     DBNGZW010000638.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7063)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7063)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7063
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_113783"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2265
                     /locus_tag="AC16JC_14910"
     CDS             <1..2265
                     /locus_tag="AC16JC_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166039.1"
                     /translation="TVSGRLPELGQLAYAGGGIIYPIGGSIVRYLGTTYGDWRIAALY
                     HELYKYPTLEAAFRELYGRTLAQLSDEWQFWMRRRYFGDVGTTRPLALVAHQLTTLAI
                     KPVAYRLPDDSVMRVLYFSPADGYASIYSRPLEGGSADLVIHGERTPEFESFHYFESR
                     IDVNPAGVAVFGSRFESRDALIFWDLRKGRLVGRYQFQDLVSILSPAWAPDGKSVVFS
                     GLAVSGFSDLYRLRLDDGRLERLTNDRYQDIDPSISPDGRTVVFASDRTAFGSKGARN
                     LFLLDLASGAIRDLTYGDWQDDTPRWSPATGRIWFSSDRDGTLQLYSVDSAGSGRRET
                     AALGGAFDPQFLDSASGVVFGGFADLTFNLYRAKSLPDSGLTQIALDSTRAAPGWEWP
                     ELASTTVAGATPGPYKRRYGLDFAAGEAAVAPGLGSEQGAVVLFSDLLNDHQFIGTLS
                     SFSYSGSGLGNLLENLSGSAFYLDQTHRINWGVGAYRQRGLFYEGDFTSLFQETSYGM
                     LGQVRYPFSRFRRLEAEFRLEHSNRFDFTNPVAAEPRRVAWLAANYLTYVKDNSLWLA
                     TGPIDGERYSFTVGLVNDVNHGRFDAYVLSGDVRHYLRTTRRSAIAVRALGYWTGGDR
                     PRLINIGGSWGLRGYPYFGYVSGTRAWLLSAEWRFPITDYLALGFPFGAAQFPGVQGA
                     FFTDLGRAWTPTTRGRGTLGSWGVGFRMPIIDALVLRLDVGYRYHRGDITSYGLPSRD
                     RRARFADFFFGFNY"
     gene            2277..2975
                     /locus_tag="AC16JC_14915"
     CDS             2277..2975
                     /locus_tag="AC16JC_14915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166040.1"
                     /translation="MLDRLGGGSYIAACLALAACYGRPAPLLPAPVPVIPRDSIVVWS
                     RRSGPTRPTALRFRWRYRDDRRSGGGRGTARVAPPDSVRVDWATSLSIRTGAAVVVGD
                     SLQWADPKEDYPSSVTPAVQLVWTALGVVRPPGIAATVFGAGDSVRAVWRYAEAHDTL
                     DFRQSLGVSRTLETEWRRDGAVMARGRTVLGPGGLPESVRIDVPKRSARFELTFVAVD
                     SGATFAPALWRSRR"
     gene            2905..3441
                     /gene="lptE"
                     /locus_tag="AC16JC_14920"
     CDS             2905..3441
                     /gene="lptE"
                     /locus_tag="AC16JC_14920"
                     /inference="COORDINATES: protein motif:HMM:NF016290.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HIR5166041.1"
                     /translation="MSSRSWRWILGRRSLRRCGAVAASLAGLALSGCLYGFAGGGLPN
                     IKTVAILPFDNDTPEPALTTEVASAVHEAIEGRLGLRAAGEATADAVVRGRIARYDPD
                     LPLAVQAGTGEANVTRRQVQLTVDISIVDQRDGRTLWQHQGLTILGDYQPPQEAEGRK
                     IALQKLVTEIVDGAQSQW"
     gene            3435..3812
                     /locus_tag="AC16JC_14925"
     CDS             3435..3812
                     /locus_tag="AC16JC_14925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166042.1"
                     /translation="MVTAASPPRRRGASTTGCLLSLLVLVAVLYYGVNIGELYFRYYR
                     LVDEMGSQARVAAALDNGTIQRRIATAIQEIGLPDDAVSNLQITRVAAPREITIESEY
                     SESVHLPLFDHTFRFHPKVTEPL"
     gene            3814..4293
                     /gene="rfaE2"
                     /locus_tag="AC16JC_14930"
     CDS             3814..4293
                     /gene="rfaE2"
                     /locus_tag="AC16JC_14930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014723734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HIR5166043.1"
                     /translation="MSTASKIRPREDAVRWRAAQGGPVVFTNGVFDLLHVGHVTVLEA
                     ARAEGGVLVVGINSDDSVRRLAKGDDRPLVPLAERARVLAALACVDCVVPFEEATPLE
                     LIRALRPDVLVKGADYALEEIVGAADVTGWGGRVVRIPLVAGKSTTDLVRQLREKER"
     gene            4322..4954
                     /locus_tag="AC16JC_14935"
     CDS             4322..4954
                     /locus_tag="AC16JC_14935"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006902906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HIR5166044.1"
                     /translation="MKLLVATRSPGKTREIREMLAVPSLRLSFPDELGLERLPEEDEL
                     EQTDSYIGNAVAKARYFAQHSGLPTVADDSGLEVDALGGAPGVRSARFALEQGSVAPG
                     QSLDAANNALLLARLSGVPAEQRTARYRCVVAYLAGPGETPEIVEATCEGRVLPELRG
                     SGGFGYDPLFFSEDLQRGFGEVPADIKHQVSHRGRAFRALIEVLQRRPES"
     gene            4980..5053
                     /locus_tag="AC16JC_14940"
     tRNA            4980..5053
                     /locus_tag="AC16JC_14940"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5014..5016,aa:Pro,seq:tgg)
     gene            complement(5117..5575)
                     /locus_tag="AC16JC_14945"
     CDS             complement(5117..5575)
                     /locus_tag="AC16JC_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018621465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5166045.1"
                     /translation="MNRIIVTLVALFLVYSAPATAQNPATPKGTNAEQEILDLSKTKW
                     RWMSERQVDSLAVLFDDQAVFVHMGGTMSRSQELDVIKSGGIQYKHAEIFEASVRFIG
                     STAILLNRIRLDAVVGGNEVTNPFMVTEVYVRQNGTWKLGSLSFTRLLTQ"
     gene            complement(5805..6962)
                     /locus_tag="AC16JC_14950"
     CDS             complement(5805..6962)
                     /locus_tag="AC16JC_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015241448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5166046.1"
                     /translation="MSNEREHRIARRSFLQCAGAVAAAPLLGGLARQAYAQSASSNGT
                     PAAQATGRRRLGRLEVSSIGLGVQNMRRKYETTVPYRPEMLNIIRRAFDRGVTFFDTA
                     EAYGPHECERILGEAIQSFRDRVVITSKFGWNIDLETGERRPGLNSKPDHIKRVVDGM
                     LMRLRTDRIDLLYQHRVDPEVPIEDVAGAVQELMQQGKVLHWGLSEMGLDTLRRAHAA
                     LPVTAVQNEYSMLWRGPERDVIPTCEELGIGFVPWSPLGVQFLTGWIDANTRFAPGDI
                     RAVESRFSPENLPHNLQLVTLVQRWAERKQATPAQIALAWLLGQQPWIVPIPGTTQMA
                     HMIENSGAADVQLTSSELADLNAAVRAIEVRGQRLPDQVLVFSGMEAPPKK"
BASE COUNT         1061 a         2372 c         2387 g         1243 t
ORIGIN      
        1 acggtgtccg gccgcctgcc ggagctggga cagctcgcct atgccggcgg cggcatcatc
       61 tacccgatcg gcgggagcat cgtccgctac ctcgggacca cgtacggcga ctggcgcatc
      121 gccgcgctct atcacgagct gtacaagtat ccgacgctcg aggcggcgtt ccgggagctc
      181 tacggccgta cgctggcgca gctgtcggac gagtggcagt tctggatgcg ccgccgctac
      241 ttcggcgacg tgggcaccac gcgcccgctc gcgctcgtcg cgcaccagct cactacgctc
      301 gccatcaagc ccgtcgccta ccggctgccg gacgacagcg tgatgcgggt gctctatttc
      361 tcccccgcgg acggctacgc cagcatctac agccggccgc tcgagggggg ctccgcggat
      421 ctcgtgatcc acggggagcg gacgcccgag ttcgagtcct ttcactactt cgagtcgcgc
      481 atcgacgtga atcccgccgg cgtggcggtg ttcggcagcc gcttcgagtc gcgcgatgcc
      541 ctgatcttct gggatctgcg caagggtcgt ctggtgggcc gctaccagtt ccaggacctg
      601 gtgtcgattc tctcacccgc gtgggcgccc gacggcaaga gcgtcgtgtt cagcggcctc
      661 gccgtttccg gtttttcgga tctgtaccgc ctccggctcg acgacgggcg tctggagcgc
      721 ctgaccaacg accgttacca ggacatcgat ccgtccatca gtcccgacgg gcggaccgtg
      781 gtcttcgcct ccgaccgcac ggccttcggc tcgaaaggcg cccgcaatct cttcctgctc
      841 gacctggcga gcggcgccat tcgcgacctc acctacggcg actggcagga cgatacgccg
      901 cgctggtcgc ccgcgaccgg ccgcatctgg ttctcgagcg accgggacgg gactttgcag
      961 ctctactccg tggattcggc ggggagcggc cggcgcgaaa cggccgccct gggcggcgcg
     1021 ttcgacccgc agttcttgga cagcgcgtcc ggtgtcgtgt tcggcggatt cgccgacctc
     1081 acgttcaatc tgtaccgggc gaaatcgctg cccgactccg ggctcacgca gatcgcactc
     1141 gacagtacgc gagccgcgcc cgggtgggaa tggccggagc tcgcgtccac gaccgtggcc
     1201 ggcgcgacgc cgggacccta caagcgacgc tacggactcg acttcgccgc cggcgaagcc
     1261 gcggtggctc ccgggctggg gtcggagcag ggggccgtgg tcctgttcag cgatctgctc
     1321 aacgaccacc agttcatcgg gacgctctcg tcgttctcgt attccggcag cggtctcggg
     1381 aacctgctgg agaatctcag cggctccgcg ttctatctcg accagaccca tcgcatcaac
     1441 tggggcgtcg gcgcctaccg gcagcgcggg ctgttctacg agggagattt caccagcctg
     1501 ttccaggaga cgagctacgg catgctgggc caggtacgct atcccttttc gcgattccgc
     1561 cggctcgagg cggagttccg gctggagcat tcgaaccgct tcgatttcac caatccggtc
     1621 gctgccgagc cgaggcgtgt cgcctggctg gccgcgaact acctcaccta cgtgaaggac
     1681 aacagcctgt ggctggccac tggtccgatc gacggtgagc ggtacagctt cacggtgggg
     1741 ctcgtcaacg acgtcaacca cggccggttc gacgcctacg tcctctccgg ggacgtgcgg
     1801 cactacctcc gcaccacgcg gcgcagcgcg atcgccgtgc gcgcgctcgg ctactggacg
     1861 ggcggcgatc gccctcgcct catcaacatc ggcggctcct gggggctgcg cggctatccg
     1921 tatttcgggt acgtctccgg cacgcgcgcc tggttgctct ccgccgaatg gcgctttccg
     1981 atcaccgact acctggccct gggatttccc ttcggggccg cgcagtttcc cggggtgcag
     2041 ggagcgttct tcaccgatct cgggcgtgcg tggacgccga cgacacgcgg ccgcgggacg
     2101 ctcggcagct ggggagtggg attccgcatg ccgatcatcg atgcgctggt gctgcggctc
     2161 gatgtgggct atcgctacca ccgcggcgac atcacgtcgt acggcctgcc ctcccgggat
     2221 cgacgcgcac ggttcgcgga cttcttcttc gggttcaact attgagcggc cggcgcttgc
     2281 ttgaccgcct tggcggcggt tcctacattg ccgcatgtct cgcgctggcc gcctgttacg
     2341 gccgcccggc accgctcctg ccggcgccgg tgcccgttat tccgcgcgac agcatcgtgg
     2401 tgtggagccg gcgcagcgga ccgacgcgcc ccacggcgct gcggttccgg tggcgctatc
     2461 gcgacgaccg gcgcagcggc ggcggccggg gcacggcccg ggtcgcaccc ccggattcgg
     2521 tgcgggtcga ttgggcgaca tcgctcagta tccggacggg tgcggcggtg gtggtgggcg
     2581 actcgctgca gtgggcggac ccgaaggagg actatccttc ctcggtgacg ccggccgtcc
     2641 agctcgtctg gaccgcgctc ggggtcgtgc gtccccccgg cattgcggcg acggtgttcg
     2701 gcgccggcga ctccgtccgg gcggtgtggc gctacgccga agcgcacgac acgctggact
     2761 tccgccagag cctgggtgtg tcgcggacac tcgagacgga atggcgccgc gacggagcgg
     2821 tgatggcgcg tggccgcacg gtcctcggcc ccggcgggct tcccgagagc gtacggatcg
     2881 atgtcccgaa gaggtcggca cggtttgagc tcacgttcgt ggcggtggat tctggggcga
     2941 cgttcgctcc ggcgctgtgg cgcagtcgcc gctagcctcg ccgggctcgc gctgagcggc
     3001 tgtctgtacg ggttcgccgg tggcggcctc ccgaacatca agaccgtcgc gatcctgccc
     3061 ttcgacaacg acacgccgga gccggcgttg accacggaag tggccagcgc cgtgcacgaa
     3121 gcgatcgagg gacggctcgg gctgcgcgcc gccggtgagg ccacggccga cgccgtcgtg
     3181 cggggacgga tcgcccgcta cgatcccgat ctgccgctcg cggtgcaggc cggcaccggc
     3241 gaggccaacg tcacgcgccg gcaggtccag ctgaccgtgg acatttcgat cgtggatcag
     3301 cgcgacggtc ggaccctctg gcaacatcag ggcctcacca tcctcggcga ctaccagccc
     3361 ccgcaggagg ccgagggccg caagatcgcg ctgcagaagc tcgtcaccga gatcgtggac
     3421 ggagcgcaat cacaatggtg accgcagcct ctcccccgcg acggcgcggc gccagtacga
     3481 ccgggtgcct gctgtcgctc ctcgtgctcg tggcggtgct ctactacggc gtcaacatcg
     3541 gcgagctcta tttccgctac taccggctcg tagacgaaat gggctcacag gcgcgggtag
     3601 cggcagctct cgacaacggc acgatccagc gtcgcatcgc gacggcgatc caggagatcg
     3661 gcctccccga cgacgccgta tcgaacctgc aaatcacccg ggtcgccgcg ccacgggaga
     3721 tcacgatcga aagcgagtac tccgagtcgg tccatctgcc gctgttcgat cacactttcc
     3781 gattccatcc caaggtcacc gagccgctgt gaggtgagca ccgcctccaa gatccggccg
     3841 cgggaggacg ccgtacgctg gcgcgcggcc cagggcggtc ccgtggtgtt caccaacggg
     3901 gtcttcgatc tgctccacgt gggacacgtg acggtgctgg aagcggcgcg cgccgagggc
     3961 ggggtgctcg tggtgggcat caactcggac gattcggtgc gccggctggc caaaggcgac
     4021 gaccgcccac tggtaccgct ggccgagcgc gctcgggtgc ttgccgccct ggcgtgcgtc
     4081 gattgcgtcg tccccttcga ggaagccacg ccgctcgagc tgatccgggc gctccggccg
     4141 gacgtgttgg tgaagggggc cgactacgcg ctggaggaga tcgtcggggc ggcggacgtc
     4201 accggctggg gcgggcgggt cgtgcggatt cccctcgtcg ccgggaaatc caccactgat
     4261 ctcgtgcggc aattgcgtga gaaggagcga tgaatccgca atcggcaatc cgcaatccac
     4321 aatgaaactg ctcgttgcca cgcgcagtcc ggggaagacg cgcgagatcc gtgagatgct
     4381 ggcggtcccg tcgttgcggc tcagcttccc cgacgagctc gggttggagc ggctgccgga
     4441 agaggacgag ctggagcaga ccgacagcta catcggcaac gcggtggcca aggcacggta
     4501 cttcgcgcag cacagcggcc tgccgaccgt ggccgacgat tccggcctgg aggtcgacgc
     4561 gctcggcggg gcgccgggcg ttcgttcggc acggttcgca ctcgagcagg ggagcgtagc
     4621 gcccggccag tcgctcgacg ccgccaacaa cgcgctgctg ctggcgcgcc tgtccggcgt
     4681 cccggccgag cagcggacgg cccggtaccg gtgcgtggtc gcctatctcg cgggcccggg
     4741 agagacgccc gaaatcgtcg aggccacctg cgaggggagg gtcttgccgg agctccgcgg
     4801 cagcggcggc ttcggctacg atcccctgtt cttctccgag gacttgcagc gcggtttcgg
     4861 cgaggtgccc gcggacatca agcaccaggt atcgcaccgt gggcgggcgt tccgcgcgtt
     4921 gatcgaggtg ctgcaacgtc gcccggagtc ctagttatgt tgtggtcgcc tccggttctc
     4981 ggggcgtagc gcagtctggt agcgcgtctg gtttgggacc agaaggtccc gggttcaaat
     5041 cccggcgccc cgactcccga aggtgctgca ccatggcgtt tccgtactgg ccgtgtgctc
     5101 caacggctgc cgttgactat tgcgtcaaga gcctggtgaa cgagagcgag cccaacttcc
     5161 acgtcccgtt ctgccgcaca tacacctccg tcaccatgaa aggattggtg acctcgttgc
     5221 cgccaacgac ggcatccagg cgaatcctgt tcaacaggat ggccgtagac ccgatgaacc
     5281 gcaccgacgc ttcgaaaatc tccgcgtgct tgtattgaat tcctccgctc ttgatgacgt
     5341 cgagctcctg gctcctggac atggttccgc ccatgtgaac gaagacggcc tggtcatcga
     5401 agaggacagc cagtgaatcg acttggcgct ccgacatcca gcgccacttc gtcttcgaga
     5461 gatccagtat ttcctgttcg gcattcgtgc ccttcggtgt ggcgggattc tgtgccgttg
     5521 ccggcgccga gtagaccagg aagagggcga cgagcgtgac gatgatccgg ttcatttcag
     5581 tccggccctt tcttgtgaag aaacgcggac agcaggtcca cgatggtgac gttgttgagg
     5641 tccgacatcg ggaaatgtgt attgccacga acgcgacggg tccgcttcga tttcggcggc
     5701 ggtgtagaac tcgtggaaca ccgttcgata cgcgcggccg cgtcgggtga tcactcggag
     5761 tccatagtgc acacacggca gtgagggtga gaacggcggc ccgctcactt cttcggcggt
     5821 gcctccatgc ctgagaagac cagcacctgg tccggcagac gctgcccccg gacctcgatc
     5881 gctcgaacag ccgcattcag atcggccagc tcacttgacg tgagttgtac gtcagctgcc
     5941 ccgctgtttt cgatcatgtg agccatctgc gtcgtccccg ggatcggcac gatccacggt
     6001 tgctgcccga gcagccacgc gagcgcgatc tgggcgggcg tggcctgctt ccgctcggcc
     6061 catcgctgga ccagcgtcac gagctgcagg ttgtgcggca ggttctccgg cgagaagcgc
     6121 gactcgacag cgcggatgtc gccgggcgcg aagcgcgtat ttgcgtcgat ccagccggtc
     6181 aggaactgca cgccgagcgg gctccacggc acgaacccga tgcccagttc ctcacaggtc
     6241 gggatcacgt cccgctcggg cccgcgccac agcatcgagt actcgttctg cacggcggtg
     6301 accggcaatg cggcgtgcgc gcggcgcagc gtgtcgagcc ccatctccga gaggccccag
     6361 tgcaggacct tcccctgctg catcagctcc tgaacggcgc cggcgacgtc ctcgatcggg
     6421 acctccggat cgacgcggtg ctggtagagg aggtcaatgc ggtcggtgcg aaggcgcatg
     6481 agcatcccgt cgacgacacg cttgatgtgg tcgggcttgc tgttgaggcc cggacgccgc
     6541 tcgccggtct cgaggtcgat gttccaacca aacttggacg tgatgacgac cctgtcgcgg
     6601 aaagactgga tggcttcgcc gaggatgcgc tcgcactcgt gcggcccata ggcttccgcc
     6661 gtatcgaaga aggtgacgcc acggtcgaag gcccgccgga tgatgttgag catctccggg
     6721 cggtagggca ccgtcgtctc gtactttcgc ctcatgttct ggacgccgag tccgatactc
     6781 gacacttcga gcctgccgag ccggcgccgg ccagtcgcct gggcggcagg cgtcccgttc
     6841 gacgatgccg actgtgcgta cgcctgcctg gcgaggccgc cgagcagcgg cgcggcggcc
     6901 acggcgcccg cgcactgcag gaaggagcgg cgcgcgatcc tgtgctcgcg ttcatttgac
     6961 atatccaggc gtccttcgat acggattgtg gtgcttcgtc gcgtcaattt cttgccaagt
     7021 tgcgcggcaa taagagcaac gagactcctc actcccttat ctt
//
LOCUS       DBNGZW010000639         3128 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1817372, whole genome shotgun sequence.
ACCESSION   DBNGZW010000639 DBNGZW010000000
VERSION     DBNGZW010000639.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1817372"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1493
                     /locus_tag="AC16JC_14955"
     CDS             <1..1493
                     /locus_tag="AC16JC_14955"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5166047.1"
                     /translation="RPAPRPASARGEAARVPEVVLPAGHLSRRAAAPSVQAGPVTPTW
                     WVVFKKELGDLWIGGRALQLTVAYTVVLGIYTYITARDSTLSLVPPKEMVYELTKAAM
                     VACVFVGLIIGADSLSGERERATLEGLLLTPTSRRQIVIGKFLAAASPGPAALAITVP
                     YMRVLSQGDEVFAQAVLWGAGLACVLTFAFTALAMFVGFWCNSNKTSLFVSLCLYLLF
                     LLPTQLPGHAQGGTMGLFGQWINPLAAPRYFLASLLVNNWTLDRAWSWLLSPMAFAVL
                     TFGLLFWYAGPDLRLEAGKTRRVLGPKARAAVVLVLVSLLSFSSPATVLSQEPAPAAQ
                     MAEAQLQVSIDVTAKTVRAGTPLIYNTVVANTGTTATPPLIVAMNIINLNASGDVVDP
                     EDWSPQRTQYSDPIAPGQSATLNWRINAILDGNYMVYMVVIPAPSGPEETSHPVTSPG
                     IHLIVTKYTRLNPGGVLPYAIGGPVLLGLIIFFVYRQRHRQIDAGS"
     gene            1510..1845
                     /locus_tag="AC16JC_14960"
     CDS             1510..1845
                     /locus_tag="AC16JC_14960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HIR5166048.1"
                     /translation="MRVRTGFITTVLAGLLTLSAVRGVAAQQQAPAKRNYQREVPAAL
                     VPQAKISEDSSLALALAHVAHGVPDAVELVRHSGNLEWVWDIKVPGKRGITEVTVNAM
                     TGKLTSHEE"
     gene            1850..2236
                     /locus_tag="AC16JC_14965"
     CDS             1850..2236
                     /locus_tag="AC16JC_14965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166049.1"
                     /translation="MLGQALAAAVACVADDPPRPADESHGVLPEACLVEQFAPPTANR
                     NEWKPLERGTRFVHAVTRGAADSRPVNEAGPKSWTPPIRIALDAAGLDRVRDRAMAID
                     RDKYQRRPRAYGNTPPAERAPPATID"
     gene            2258..2503
                     /locus_tag="AC16JC_14970"
     CDS             2258..2503
                     /locus_tag="AC16JC_14970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166050.1"
                     /translation="MIRRVLEASLRLRVPVIAAAAAVMIVGIGRMPRPPVDVLPEFAP
                     PYIEIQTEALDRSANEVEGLVTRCLRFARQQPVEAAS"
     gene            2500..>3128
                     /locus_tag="AC16JC_14975"
     CDS             2500..>3128
                     /locus_tag="AC16JC_14975"
                     /inference="COORDINATES: protein motif:HMM:NF028240.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HIR5166051.1"
                     /translation="MTNPLRFSLVLCALQASRLGAQTAPALRFNGDFRVRYEYTSAGN
                     GALALGREVVRFRLGLTYPLRPDITVRARVATGDPGDPNSTDVTLSSFVDDLAFTLDV
                     ASVEITKPHWAAFAGKFFNPLQYTELVWDGDVNPQGVAARGMLGDRAKLSATLTGLYF
                     IVDQQANNLSSDMGGGQLTVSAPAGSRWRMSGSAGYYDYRIRSLTAANSG"
BASE COUNT          497 a         1061 c         1050 g          520 t
ORIGIN      
        1 cgcggccggc gccacgtccc gcgtccgcgc gcggggaggc cgcccgcgtg cccgaggtgg
       61 tcctgcccgc cggccacctt tcccgtcggg ccgctgcccc atcggtgcag gcgggtccgg
      121 tgacacccac gtggtgggtc gtcttcaaga aggagctcgg cgacctctgg atcggtggac
      181 gggcgctgca gctcacggtg gcctacaccg tcgtgctcgg gatctacacc tacatcacgg
      241 cgcgtgacag cacgctgagc ctcgtccccc caaaagagat ggtctacgag ctcaccaagg
      301 ccgcgatggt ggcctgcgtg ttcgtggggt tgatcattgg cgccgacagc ctgagcggcg
      361 aacgggagcg cgccaccctc gagggcctgt tgctgacgcc cacgagtcgc cggcagatag
      421 tgatcggcaa gttcctcgcg gctgcctccc ccgggcccgc ggcgctggct atcaccgttc
      481 cctacatgcg ggttctttct cagggcgacg aggtgttcgc ccaggcggtc ctgtggggag
      541 cgggcctggc atgcgtcctc acgttcgcgt tcaccgcgct ggccatgttc gtcggcttct
      601 ggtgcaactc gaacaagacg agcctgttcg tgagcctgtg cctgtatctc ctgttcctcc
      661 ttccaaccca attaccaggg catgcccagg gcggcactat gggactcttc ggtcaatgga
      721 taaacccgct ggcggcgccg aggtacttcc tcgccagcct gctggtgaac aactggacgt
      781 tggaccgggc atggtcctgg ttgctgtcgc cgatggcatt cgccgtgctc acgttcggcc
      841 tgctgttctg gtacgcgggt ccggacctgc gcctcgaggc cggaaagacg cgccgtgtcc
      901 tgggccccaa ggcgcgggcc gcggtcgtac tggtgctggt ttctctcctg tcgttctcgt
      961 ctccggccac ggtcctgagt caggaaccag cgcctgcggc gcagatggcc gaggcgcagc
     1021 tgcaggtatc gatcgacgtg acggcgaaga cggtccgggc gggaacgccg ctgatctaca
     1081 acaccgtcgt ggccaacacc gggacgaccg cgacgccgcc gctcatcgtg gcgatgaaca
     1141 tcatcaacct caatgcatcg ggcgacgtgg tggatccgga ggactggtcg ccccagcgga
     1201 cgcagtactc ggacccgatc gccccgggcc agtccgccac gctcaactgg cgcatcaacg
     1261 ccatcctcga cggcaactac atggtgtaca tggtggtcat tcccgcgccc agcggcccgg
     1321 aagagaccag ccacccggtg accagtcccg gcattcatct catcgtgacg aagtacacca
     1381 ggctcaaccc cggcggggta ctgccctacg ccatcggcgg gccggtcctg ttgggtctga
     1441 tcatcttctt cgtgtaccgg caacggcacc ggcagatcga cgccggctcc tgagggagga
     1501 ggaatgcaga tgcgagttcg taccggattc atcacgaccg tcctggcggg cctgctgacg
     1561 ctctcggctg tgcggggggt tgccgcgcaa caacaggctc cggccaaacg caactaccag
     1621 cgtgaagtcc cggcggcgct cgtgccccag gcgaagatca gcgaggacag ctcgctcgcc
     1681 ctggccctgg cccatgtggc ccacggtgtt ccggatgccg tcgagctggt acgccattcg
     1741 ggcaacctgg agtgggtgtg ggacatcaag gtccccggca agcgcggcat caccgaggtg
     1801 accgtcaacg cgatgaccgg caagctgacg agtcatgagg agtaggaggg tgctcggaca
     1861 ggccctcgcg gcagccgtcg cctgcgtcgc ggatgatccg ccgcggccgg ccgatgagag
     1921 ccacggcgtg ttgcccgagg cgtgtctcgt cgagcagttc gctcctccga cggcgaaccg
     1981 caacgagtgg aagccgctcg agcgggggac ccggttcgtc cacgctgtga cgcgcggcgc
     2041 ggccgacagc cgccccgtga acgaagcggg gccgaagagc tggacgccgc cgatacggat
     2101 cgccctggac gcggcgggtc tcgaccgcgt gcgtgaccgc gcgatggcaa tcgaccgcga
     2161 caagtaccaa cgcaggccac gtgcgtacgg caatacacct ccggcggaac gcgcgccgcc
     2221 ggccaccatt gactgagtca ctcagagggg atccgccatg atacgtcgtg tactcgaagc
     2281 gagtcttcgg ctccgggtcc cggtcatcgc cgctgcagcg gcggtgatga tcgtcggcat
     2341 cgggcgcatg ccccgaccgc cggtggacgt gctgcccgag tttgcccccc cctacatcga
     2401 gatccagacg gaagcgctcg accgttcggc gaacgaggtc gagggcctcg tcacgcggtg
     2461 cctgcgcttc gctcggcaac aacctgtgga ggctgcgtca tgaccaatcc gctccgcttc
     2521 tcgctggtac tctgcgcgct ccaggcgagc cggttggggg cgcaaaccgc gcccgcactc
     2581 cgcttcaacg gggatttccg ggtccgctac gagtacacga gcgccggaaa cggcgcgctg
     2641 gcgctgggac gcgaggtggt ccggttccgc ctcgggctca cgtatccgct gcggccggac
     2701 atcaccgtcc gcgcgcgcgt cgccaccgga gacccgggcg atcccaattc caccgatgtg
     2761 acgctcagct cgttcgtcga cgacctggcg ttcaccctcg atgtggcgtc ggtggagatc
     2821 accaaacctc attgggcggc gttcgcgggg aagttcttca atccactgca atacaccgag
     2881 ctggtgtggg acggcgacgt gaacccgcag ggtgtggccg cccggggcat gctgggcgac
     2941 agggcgaaac tctcagccac cctgacgggt ctctacttca ttgtcgacca gcaggcgaac
     3001 aatctcagca gcgacatggg gggcggccag ctcaccgtca gcgcgccggc gggttcgcgg
     3061 tggaggatgt cggggtcggc gggctattac gactaccgca tccgcagtct cacggcggcc
     3121 aacagcgg
//
LOCUS       DBNGZW010000640        12195 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_671054, whole genome shotgun sequence.
ACCESSION   DBNGZW010000640 DBNGZW010000000
VERSION     DBNGZW010000640.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 12195)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12195)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12195
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_671054"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1713
                     /locus_tag="AC16JC_14980"
     CDS             235..1713
                     /locus_tag="AC16JC_14980"
                     /inference="COORDINATES: protein motif:HMM:NF019307.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase dimerization domain-containing
                     protein"
                     /protein_id="HIR5166052.1"
                     /translation="MRRPSLLALAWLAVAGTGLAAQTTDTEREAAAQILRRIDSLETR
                     LQPTRTAERLAARADAARDRLLARVETVWTGEMQGLSDWIGHNPEVGWQEFKAFDTLT
                     AVLRRYGFQVQDSVGGLKTAFTATWTSPAGSQGPTLGLIAEYDALRGTQGAFHGDQHN
                     AQSPVAFAAARALMEYMTEAKVPGRIIVYGTPAEEVDPPAKYILWKAGVFKGADILVR
                     SHSTQETRRDRPGFGVCCLNIDAVKYTFTGRPAHQLASWNGRNALEAAVQFYSAVDHL
                     RSTWRADARVQGVIPEGGVAPNVVPDRAVVDYFIRFPDEVYLEHISRMMDDAARGAAQ
                     MTETQVAVTRYGEYRDGVTTAMLEELFYAYARKLNAPGLEEEKGRPAGYEETGFVSRE
                     IPGIGITVRSSSYPNHTYGMRDDAFIDIGHRGFLLDAKIEAAVLYDFLTQAGFRSAVA
                     EEHRVLSGLQNGYLAQLRRVYGPEIGADTTLSKSSRMEIRVP"
     gene            1807..3168
                     /locus_tag="AC16JC_14985"
     CDS             1807..3168
                     /locus_tag="AC16JC_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014068041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR5166053.1"
                     /translation="MTESASLAVATEAPGLYQDLHPPLEEWLAVREADRCLQCGGPTA
                     LAPCVVACPARVDVPGFVAALARRDVETAAALIFAANPLGGTCARVCPGEELCEGACV
                     LDTQGRRPVPVTRLQRWATDQARGWTVEEPRRAAATGRRVTVIGAGPAGLACAAELAR
                     LGHAVTVVDARPEVGGLVRYAIAPYRLVRDPLPAEALRLERTGVRFLMGQLIDSPQRL
                     RALADSADAVFLGVGLGEDQAVRYPGDELTGVWDSLPFIEAIKLQRPPQVGERVVVVG
                     GGNTAIDVAREAVRLGASTVTMVYRRSEAEMPAYPHEVVEARDEGVAFTFLAEPVRLL
                     GNGRLEAVECRRMRLGAPDRSGRPRPEPEPGSEFTIPADALVKALGQKRRTDFFSWIE
                     GLNTDGGLVAIDPATGQTSAPKFFAGGDAVNGGATVVEAVRHGKVAALGIDRYLAAQG
                     RET"
     gene            3165..3425
                     /locus_tag="AC16JC_14990"
     CDS             3165..3425
                     /locus_tag="AC16JC_14990"
                     /inference="COORDINATES: protein motif:HMM:NF024634.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR5166054.1"
                     /translation="MKPGFERYAQGDAALLVNRAWCKGCNVCVAACPSGILALDAVQR
                     IVVTDINRCIFCGVCAVRCPDFVFILERPPMQALERAAEAAA"
     gene            3422..4534
                     /locus_tag="AC16JC_14995"
     CDS             3422..4534
                     /locus_tag="AC16JC_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012063760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR5166055.1"
                     /translation="MRLIQGNEACALGALRAGCRFYAGYPITPATEIMEWMARLLPPR
                     GGVFIQMEDEIGALAAVIGASWAGAKAMTATSGPGFSLMQEHIGYAAMTETPCVIVDS
                     QRAGPSTGLPTLPSQGDVMQARWGSHGDRAAIVLTAASVPDVYEVTVAAFNLSETYRI
                     PVILLLDASLSHMRENVELPEVTVVDRRRPPSLEGFRPFAGTEFVGFGEGAELVVTGL
                     AHADSGRPRASDGPNLERMLRRSIDRLSAGEAEILRYRTRALEDADVAVLAYGITARA
                     AAGAVDQLRAEGVRAGLLELQTLWPFPERLVAELAGRVEGLVVPELNLGQLVMPVRAA
                     VNGATPVHPLGRADGHLFTPEEIGQAARAAMEAVHA"
     gene            4527..5360
                     /locus_tag="AC16JC_15000"
     CDS             4527..5360
                     /locus_tag="AC16JC_15000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012176434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5166056.1"
                     /translation="MPRVGGDVRRYLRVEAMPHVWCPGCGHGQVAHGLATALERVAVP
                     LDRLVLVGGIGCSGRTPFYFNANAMHTTHGRALAFATGIKLTRPDLTVITTMGDGDTA
                     AIGGNHFIHACRRNIDVTAIVYNNGIYGMTGGQHAPTTPLGAKSATTPLGSLEPPFDI
                     VQLALAAGASFVARTTTFDFEELPTLIAEAIRHPGFAVVEVLTQCPTYFGRLNQLGDA
                     VAMLDYERDLTLPQEEITKESLAGRMPLGVFRREVRAEYTAQYAALCARQKAAKAEAV
                     P"
     gene            5357..5926
                     /locus_tag="AC16JC_15005"
     CDS             5357..5926
                     /locus_tag="AC16JC_15005"
                     /inference="COORDINATES: protein motif:HMM:NF013707.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HIR5166057.1"
                     /translation="MTRSLPDPIQVRFAGAGGQGVILAGVLIAEAGMHDGLHVVATQS
                     YGPEARLGAAKTEVVLSHRPIVFPEVRIPDLIVCLSEEAYRKHARPLAAGGVRLVEET
                     AVAPDAQLEPGTVTAPLLATARGLGPNGAVAANVVALGALVALSEVVTEESLRRALRQ
                     RVKPALLELNERALEAGLALGRPAATRAR"
     gene            5923..6894
                     /locus_tag="AC16JC_15010"
     CDS             5923..6894
                     /locus_tag="AC16JC_15010"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017876189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR5166058.1"
                     /translation="MIVFALGSNSEFGATVSRALGVPLAPHEERRFEDGEHKCRPLAS
                     VRGRDVFVVESLYGETGESGNDKLCRLLFFIGALKDAAAARVTALVPYLCYSRKDRKS
                     KPRDPVTTRYVATLFEAVGTDRVVTLDVHNLAAYQNAFRCGTDHLEARPLFVDALAPR
                     VGDAAVAVVSPDVGGVKRADAFRESLARALGREVPLAVMEKARSEGVVTGDALVGDVA
                     GRVAILVDDLISTGTTLARAAAACRTRGAVRVIAAATHGLFTGDANARLGGPALDEVL
                     VTDSVPPFRLTDPAVRQKLTTLDVGPLFAEAARRIHEHGSLVGLLGS"
     gene            complement(6903..7922)
                     /locus_tag="AC16JC_15015"
     CDS             complement(6903..7922)
                     /locus_tag="AC16JC_15015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166059.1"
                     /translation="MTATIPAIGLDDKVAFLKRPESYPEAPGGVEALETHLAWVFLTD
                     QFAYKLKKPAHFPYVDFRTLAARRRDAEEEVRLNRRLAAGVYLGVVPLTVDEAGGLTL
                     EGGGAPVEWLVKMRRLPRESMLDFAIASRTVEASALDGVVGLLAAFYGTARSVVLAPG
                     AYRRRFEQEIDANARATAERRYGLPSAVITATAAAQRRFLLQRADLLEARAAAGRIVE
                     GHGDLRPEHVSLGPPPVIIDCVEFKRGFRLLDPADELAYLGMECERLGAETLGPGLLA
                     RYGALTGDQPPAALIGFYRSFRALLRAKLAAWHLDDRTVPYPSQWFNRARSYLALAER
                     YARQP"
     gene            complement(7954..8235)
                     /locus_tag="AC16JC_15020"
     CDS             complement(7954..8235)
                     /locus_tag="AC16JC_15020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166060.1"
                     /translation="MTPRKKLTAAVKQAAARARAAGRTAAHRIAVKTDEVLVDAGKAA
                     KQRQRGRAVKSVLKTATRAAAIAAATATTIMAARAAARARRRRAAAAAH"
     gene            complement(8232..8417)
                     /locus_tag="AC16JC_15025"
     CDS             complement(8232..8417)
                     /locus_tag="AC16JC_15025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166061.1"
                     /translation="MATTKTDGAQLLRKLEALERRLDTMGDRVKSLERHTTELDELRR
                     EIGNLKIEVEEFREGES"
     gene            complement(8509..8964)
                     /locus_tag="AC16JC_15030"
     CDS             complement(8509..8964)
                     /locus_tag="AC16JC_15030"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015720984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5166062.1"
                     /translation="MAKVSAGLLVYRKRQGTLEVLLAHPGGPFWANRDSGAWSIPKGE
                     VDAPEPPLDAARREFHEETGFTVSGNFTALRLLRQPGGKVVHAWAVEGDCDAAQMRSN
                     TFRMEWPPRSGKQQEFPEIDRVEWFSIDEALRRILPGQAGFLRELATLV"
     gene            complement(9016..10581)
                     /locus_tag="AC16JC_15035"
     CDS             complement(9016..10581)
                     /locus_tag="AC16JC_15035"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HIR5166063.1"
                     /translation="MSATARWRAEVVPALNLLLFGLALWALDRLLHDYPLRDIFAALQ
                     AIPFSLILLAALISGLGYLALIGYDWVAFRFLGLDLPLHRMLIPSFVSFAVSNNAPAS
                     MVTAGGVRYRMYRTLGLTATGAVTVAGFNVVTYALGLAGLAGLALLIDPTGAGAHTFA
                     LPGRALGVLLLAGVAAYLLLARLGDGAVRVLGRTFALPSLRIALAQLGISVADWLLSS
                     APLFILLAGVTAVPHFSFLTAFLVAAFATLFLPIPGGVGVFEAVVLLLRPSSVPVPDA
                     LAALLVYRLVYYLVPLAVAGGLLAARAVQRVRRSEHPVRELLRGASSLAPRVLAFTTF
                     LAGLVLLVTGAIPADDRRLAWLGRLLPLGVIEASHFLSSIVGAVLVLSAWGLERRSHV
                     AYHMVRMLFGAGILLTLLRSFDLRLTVFLAITLAVLILAGREFPRPGSLTREPMGYRW
                     AFAAGAAFLVEAWIGALLYRHVQFAGQVWWRFALSGQGPRALRAAVGASIVMLLFAVE
                     RLISGTVPRSRTP"
     gene            complement(10583..10777)
                     /locus_tag="AC16JC_15040"
     CDS             complement(10583..10777)
                     /locus_tag="AC16JC_15040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166064.1"
                     /translation="MRGTGAAHAMGLLMDRLLPLVTSETAVPSHGLDPSIVHRADVTG
                     RPAVVYRIVIGERTGRFEKR"
     gene            complement(10818..10895)
                     /locus_tag="AC16JC_15045"
                     /pseudo
     CDS             complement(10818..10895)
                     /locus_tag="AC16JC_15045"
                     /inference="COORDINATES: protein motif:HMM:NF024304.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
     gene            10981..11973
                     /locus_tag="AC16JC_15050"
     CDS             10981..11973
                     /locus_tag="AC16JC_15050"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017775722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR5166065.1"
                     /translation="MTRRKIPDAPGAAPLEARIPPPAHPHRRQVPLPWNQPKSPADDP
                     DAPRRLQAILDSPGYRQADQDVEFLNRDETRGLRLQVDYFKPEMLLEEHGIHDTIVVV
                     GSTRILEPGAARRQVATLREALAGNPCDRELASQLAVAERILANSDYYEVAREFGRLV
                     GDGGGGAVRAVIMTGGGPGIMEAANRGAFDAGAKSIGLNITLPHPQFPNPYITPELCF
                     RFHYFVLRKLHFLLRARALVAFPGGYGTFDEVFEALALVQTRKIRPFPIVLVGERFWR
                     RAFDIDFLVEEGVIEPEDRDLFWFAETAPDILGGIRHWHTLTAAEPQVSAAYRL"
     gene            complement(12029..12193)
                     /locus_tag="AC16JC_15055"
     CDS             complement(12029..12193)
                     /locus_tag="AC16JC_15055"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5166066.1"
                     /translation="MVRAGVHRLIVTEEGRLVGVLSSMDIVRAVAQQRLVAAGPEVRS
                     EPRSWAEVRR"
BASE COUNT         1837 a         4214 c         4381 g         1763 t
ORIGIN      
        1 cctacccggc cagaccacga tctcgcgcgt gccgctgttc gactcccggc tccaggacta
       61 tttctggaac tacgacaaca acggtctgaa gctcgcccag ttccgcttct atcccgtgtc
      121 cacgaccgtt ccgtaagcaa ccgaggcaag gcgccgccgc tcgtcctggt gtcgccttgc
      181 ctccccgcgg tcctcgcacg accttctcgc ggtcccccac cagagggaac cgcaatgcgc
      241 cgtcccagcc ttctcgccct ggcctggctc gccgtcgctg gcacggggct ggccgcccag
      301 acgacggaca ccgagcggga ggccgccgct cagatcctgc gccggatcga ctcgctcgag
      361 actcggctgc aaccgacgcg caccgccgag cggctcgccg cccgcgccga cgcggcgcgc
      421 gaccgcctgc tggcgcgcgt ggagacggtg tggaccggcg agatgcaggg actctccgac
      481 tggatcggcc ataacccgga agtcggctgg caggaattca aggccttcga caccctcacg
      541 gcggtgttgc ggcgctatgg ctttcaggtt caggacagcg tcggcggact gaagactgcg
      601 ttcaccgcta cctggacctc acctgccgga tcgcagggtc ccacgctcgg tctcatcgcg
      661 gagtacgacg ccctgcgcgg cacccagggc gcgttccacg gcgatcagca caatgcgcag
      721 agcccggtcg ccttcgcggc ggcgcgggcg ctcatggagt acatgaccga ggcgaaggtg
      781 cccgggcgca tcatcgtcta cggcacgccc gccgaggagg tcgatccgcc cgcgaagtac
      841 atcctgtgga aggcgggggt cttcaaggga gccgacatcc tggtgcgcag ccactccacc
      901 caggagacgc gtcgcgaccg tcccggattc ggcgtgtgtt gcctcaacat cgacgcagtc
      961 aagtacacgt tcaccggtcg cccggcgcac cagctcgcct cgtggaacgg ccgcaatgcg
     1021 ctggaagcgg cggtgcagtt ctattccgcg gtggatcacc tgcgctccac ctggcgcgcc
     1081 gacgcccgcg tccagggagt gattcccgag ggcggggtcg cgcccaacgt cgtgccggac
     1141 cgcgccgtgg tggactattt catccggttc cccgacgagg tctatctcga gcacatctcc
     1201 cgcatgatgg acgacgccgc tcgcggcgcc gcccagatga cggagaccca ggtcgcggtc
     1261 acgcgctacg gcgaataccg ggacggggtc accacggcga tgctggagga gttgttctat
     1321 gcctacgcca ggaagctgaa cgcgccgggg ctggaggagg agaagggccg gcccgcgggc
     1381 tacgaggaaa ccggcttcgt gagtcgcgag atcccgggga tcggcatcac ggtgcggagc
     1441 tcgtcgtatc cgaatcacac ctacgggatg cgggacgatg cgttcatcga catcgggcac
     1501 cgcggcttcc tgctcgacgc caagatcgag gcggccgtgc tgtacgactt cctcactcag
     1561 gccgggttcc ggagcgcggt agccgaggaa caccgtgtgc tgagcggcct ccagaacggg
     1621 tacctggcgc agctgcgccg cgtgtatgga ccggaaatcg gggccgacac cacgctttcc
     1681 aagagcagcc gcatggagat ccgggtgccc taagactcct tcatacgcgc ttcactatcc
     1741 gattaggggg gaagtgacct cttcatagta gggaatttct cgctgccctt tcatgaagag
     1801 gctcgcgtga cggagtcggc aagccttgcg gtggccaccg aggcgccggg gctgtatcag
     1861 gacctgcacc cgcccctcga agagtggctc gcggtgcgtg aagcggaccg gtgtctccag
     1921 tgcggcggac cgacggcgct cgcgccctgc gtggtggcct gcccggcccg cgtcgacgta
     1981 ccggggttcg tcgccgcgct ggcgcggcgc gacgtcgaga ctgcggccgc gctgatcttc
     2041 gccgcgaacc cgctgggcgg cacgtgcgcc cgcgtgtgtc ccggcgagga gctgtgcgag
     2101 ggcgcctgcg tgctggacac gcaagggcgc cggccggttc cggtcacccg gctgcagcgc
     2161 tgggccaccg atcaggcgag gggatggacg gtcgaggagc cgcgacgggc ggcggcgacg
     2221 ggccgtcgcg tgaccgtgat tggggctggt cccgcggggc tggcctgtgc cgccgagttg
     2281 gcccggctgg gccatgcggt caccgtggtg gatgcgcgtc ccgaggtggg cggcctggtg
     2341 cgctacgcca tcgctcccta ccggctggtc cgcgatccgt tgcccgccga agcgttgcgc
     2401 ctcgagcgga ccggcgtgcg gttcctgatg ggtcaactca tcgacagccc gcagcggctg
     2461 cgcgcactgg cggactccgc cgacgccgtc ttcctgggag tggggctggg tgaagaccag
     2521 gccgtccggt atccgggcga cgagctgacc ggcgtgtggg actcgctccc gttcatcgag
     2581 gcgatcaagc tgcaacggcc gccgcaggtg ggtgagcggg tcgtggtggt gggcggcggg
     2641 aacaccgcga tcgacgtcgc gcgtgaggcg gttcggctcg gggcatcgac ggtgacgatg
     2701 gtgtaccggc gctccgaagc agagatgccc gcctatcccc atgaagtggt ggaggcacgc
     2761 gacgaaggcg tcgcgttcac gttcctggcc gagccggtcc gactgctggg gaacgggcgc
     2821 ctcgaggccg tggaatgccg gcgcatgcgg cttggcgcgc cggaccggtc cgggcggcca
     2881 cgcccggagc cggagccggg gagcgagttc acgatccccg cggacgccct cgtcaaggcg
     2941 ctcggccaga agcgccgcac cgacttcttc tcctggatcg agggcctcaa caccgacggc
     3001 ggcctggtcg cgatcgatcc ggccacgggg cagacgagcg cacccaagtt cttcgccggc
     3061 ggcgacgccg tgaacggcgg ggcgacggta gtcgaagccg tgcggcacgg caaggtcgcg
     3121 gcgctcggta tcgaccgcta tctcgcggca cagggacggg agacatgaag ccagggttcg
     3181 agcggtacgc gcagggggac gcggcgttgc tggtgaatcg cgcctggtgc aaggggtgca
     3241 acgtctgtgt ggcggcctgt cccagcggga tcctggcgct ggatgcggtg caacgcatcg
     3301 tcgtgacgga catcaaccgc tgcatcttct gcggcgtctg tgcggttcgc tgccccgact
     3361 tcgtgttcat tctcgagcgg ccccccatgc aggcgttgga gcgggcggcg gaggcggccg
     3421 catgagactg atccagggaa acgaggcctg cgcgctggga gcgctccggg ccggctgccg
     3481 cttctacgcc gggtatccga tcacccccgc caccgagatc atggagtgga tggcgcggct
     3541 gctcccgccg cgcggcggtg tgttcatcca gatggaagac gagatcgggg cgctggcggc
     3601 cgtcatcggc gcctcgtggg ccggtgccaa ggcgatgacc gcgacgtcgg gtcccggctt
     3661 ctcgctgatg caggagcaca tcggctacgc cgcgatgacg gagacgccgt gcgtgatcgt
     3721 ggactcgcag cgcgcgggcc cgagcaccgg cctcccgacc ctgccgtcgc agggagacgt
     3781 gatgcaggcg cgctggggat cgcatggcga ccgtgccgcg atcgtgctca cggcggcgtc
     3841 ggtgccggac gtgtacgagg tcacggtggc ggcgttcaac ctgagcgaga cgtaccgcat
     3901 ccccgtgatc ctgctgctcg atgcgtcgct gtcgcacatg cgggagaacg tcgagctgcc
     3961 ggaggtcacg gtggtggatc gccgccggcc cccgtcgctc gaaggcttcc ggccgttcgc
     4021 gggcaccgag ttcgtcggat tcggcgaggg cgcggagctc gtggtgaccg ggctcgccca
     4081 cgcggactcg gggcgcccgc gggcctcgga cgggccgaac ctcgagcgca tgctgcggcg
     4141 gtcgatcgat cgcctctcgg cgggcgaggc cgagatcctg cgctaccgca cccgcgccct
     4201 ggaggacgcc gacgtcgcgg tgctcgccta cggcatcacc gcgcgggccg cggccggagc
     4261 ggtggatcag ctgcgcgccg agggcgtgcg cgccggcctg ctcgagctgc agacgctgtg
     4321 gccgttcccc gagcggctcg tggccgagct ggcggggcgc gtcgaggggc tggtggtgcc
     4381 ggagctgaac ctgggacagc tcgtcatgcc ggtgcgcgcg gccgtgaacg gcgcgacgcc
     4441 ggtgcatcct ctggggcggg ccgacggcca tctcttcacg ccagaggaga tcgggcaggc
     4501 ggcgcgggcc gcgatggagg cggtgcatgc ctagggtcgg cggggatgtg cggcgctatc
     4561 tgcgggtcga agcgatgccg cacgtgtggt gtccggggtg cggccacggc caggtcgcgc
     4621 atgggctggc gacggcgctc gagcgcgtgg ccgtgccgct ggaccgcctg gtgctggtcg
     4681 gcggcatcgg ctgctcgggg cgcacgccgt tctatttcaa cgcgaacgcc atgcacacga
     4741 cgcacggccg ggcgctcgcc ttcgccaccg ggatcaagct cacgcggccc gacctgaccg
     4801 tcatcacgac catgggcgac ggcgacacgg ccgcaatcgg gggcaaccac ttcatccatg
     4861 cctgtcggcg caacatcgac gtcacggcga tcgtctacaa caacgggatc tacgggatga
     4921 ccggcgggca gcacgcgccg acgacgccgc tgggcgccaa gtcggccacc acgccgctgg
     4981 gcagcctcga gccgccgttc gacatcgtgc agctcgccct cgccgcgggc gccagcttcg
     5041 tggcgcggac gacgaccttc gacttcgagg agctgccgac cctcatcgcg gaggcgatcc
     5101 ggcatcctgg cttcgccgtg gtggaagtgc tcacccagtg tccgacctac ttcggccgcc
     5161 tgaaccagct cggcgacgcg gtggccatgc tcgactacga gcgcgacctg accctgcccc
     5221 aggaagagat caccaaggag agcctcgcgg gccggatgcc gctcggggtg ttccggcgcg
     5281 aggtgcgggc ggagtacacc gcacaatacg ccgccttgtg cgcccggcag aaagcggcga
     5341 aggcggaggc cgtgccatga cgcgctccct ccccgatccg atccaggtcc ggttcgccgg
     5401 cgcgggcggc cagggcgtga tcctggccgg cgtgctcatc gccgaagcgg ggatgcacga
     5461 tgggctgcac gtcgtggcga cccagagcta cggccccgag gcgcgcctcg gcgcggccaa
     5521 gaccgaggtc gtgctgtcgc accggcccat cgtctttccc gaagtgcgca tccctgacct
     5581 catcgtgtgc ctgtcggagg aggcgtaccg caagcacgcc cggccactcg ccgccggcgg
     5641 cgtgcggctg gtcgaggaga ccgcggtcgc gccggacgca cagctcgagc ccggaacggt
     5701 cacggcgccg ctgctcgcga cggctcgcgg tctcgggccc aacggcgcgg tggcggccaa
     5761 cgtggtggcg ctcggggcgc tggtggcgtt gtcggaagtc gtgaccgagg agagcctgcg
     5821 gcgggcgttg cggcagcgcg tcaagcccgc gctgctcgag ctgaacgagc gcgcgctcga
     5881 ggcggggctc gcgctggggc ggcccgcggc gacgcgcgcg aggtgattgt cttcgccctg
     5941 ggttcgaaca gcgaattcgg ggccacggtt tcgcgtgccc tgggcgtgcc gctcgcgccc
     6001 cacgaggaac ggcgcttcga ggacggcgag cacaagtgcc ggccgctggc gagcgtgcgc
     6061 ggccgcgacg tgttcgtcgt cgagtcactc tacggggaga cgggcgagag cggcaacgac
     6121 aagctgtgcc gcctgctgtt cttcatcggg gcgctcaagg atgccgccgc ggcacgtgtg
     6181 acggcgctcg tgccctatct ctgctactcc cgcaaggacc gcaagtccaa gccccgcgat
     6241 cccgtcacga cccgctacgt cgccacgctg ttcgaggcgg tggggacgga tcgcgtcgtg
     6301 acgctcgacg tgcacaatct cgccgcctat cagaacgcgt ttcgctgcgg cacggaccat
     6361 ctggaggccc ggcctctgtt cgtggatgcg ctcgcgccgc gcgtcgggga cgctgcggtc
     6421 gcggtcgtct cgcccgacgt cggcggcgtg aagcgggcgg acgccttccg cgagtcactg
     6481 gcccgcgcac tgggacgtga ggtgccgctc gccgtcatgg agaaggcgcg cagcgaggga
     6541 gtcgtgaccg gggacgcgct ggtcggggac gtcgccggtc gggtggcgat cctcgtggac
     6601 gacctgatca gcacgggaac gacgctggcg cgtgccgcgg ccgcctgccg cacccgcggg
     6661 gccgtgcgcg tgatcgcggc ggccacccac gggctcttca ccggagacgc gaatgcgcgg
     6721 ctcggcgggc cggcactcga cgaggtgctg gtgaccgaca gcgtcccgcc gttccggctg
     6781 accgaccctg cggtccggca gaagctcact accctcgacg tggggccgtt attcgcggaa
     6841 gccgcgcggc ggattcacga gcacggctcg ctggtcggtc tgctgggatc ctgaggctga
     6901 gactacggct gtcgcgcgta gcgctccgcc agcgcgagat agcttcgagc ccggttgaac
     6961 cactgtgacg ggtagggcac ggtgcggtcg tccaggtgcc aggcggcgag tttggcgcgc
     7021 agcagcgcac ggaagctcct gtagaacccg atgagcgcgg cgggcggctg gtccccggtg
     7081 agcgcaccgt agcgcgcgag cagtcccggc cccagcgttt ccgcgcccag gcgctcgcat
     7141 tccatgccca ggtaggcgag ctcgtcggcc ggatcgagca gtcggaaccc ccgcttgaac
     7201 tcgacgcaat cgatgatcac cggaggcggc ccgagactca cgtgctcggg gcggagatcg
     7261 ccgtgccctt cgacgatccg cccggcggcg gcgcgggctt cgagcagatc cgcccgttgc
     7321 agaagaaagc gccgctgcgc ggcggcggtc gcggtgatca cggcggacgg caggccgtac
     7381 cgccgctccg ccgtcgcacg ggcgttcgca tcgatttcct gctcgaaccg gcgtcggtag
     7441 gcgccgggcg cgaggacgac gctgcgcgcc gtgccgtaga aggccgcgag caacccgacg
     7501 acgccatcga gcgcgctcgc ctccaccgtt cgggatgcga tggcgaagtc gagcatgctc
     7561 tcccggggca ggcggcgcat cttgaccagc cactcgaccg gtgcgccgcc gccctcgagg
     7621 gtgagaccgc ccgcctcatc gaccgtgagc ggcacgacgc cgagatacac ccctgcggcc
     7681 agacggcggt tgagccgcac ttcctcctcg gcatcgcggc ggcgtgcggc gagggtgcgg
     7741 aagtcgacgt acggaaagtg cgccggcttc ttgagcttgt aggcgaactg gtcggtcagg
     7801 aacacccaag cgaggtgggt ctccagcgcc tccacgccgc ccggagcttc ggggtagctc
     7861 tccgggcgct tgaggaaggc gaccttgtca tccagcccga tcgcggggat cgtcgccgtc
     7921 atgctgccgc ccgcgtgcgg agcggatggg gccctagtgg gccgccgccg ctgcgcgccg
     7981 ccgccgcgcc cgggcggcgg ctcgtgccgc catgatggtc gtcgccgtcg cggcggcgat
     8041 cgcggccgcc ctggtcgcgg tcttcaggac ggacttgacg gcccgccccc gctggcgctg
     8101 cttggcggcc ttgccggcat cgaccaacac ttcgtccgtt ttcaccgcta tccggtgcgc
     8161 ggcggtgcgc cccgccgccc gcgcccgcgc agcggcctgc ttcacggccg ccgtgagttt
     8221 ctttctcgga gtcatgattc cccctcccgg aactcctcca cctcgatctt cagattcccg
     8281 atctcccgtc gcagctcgtc cagctccgtc gtgtgtcgct ccaggctctt gacccgatcg
     8341 cccatggtgt cgaggcggcg ctcgagagcc tcgagcttcc gcaggagctg cgctccatcg
     8401 gtcttcgtcg tggccatgct ctcctcctga tgcgccatgc cgtaacatca cctccggggg
     8461 tgatcgagac ctgaaacaag tatgagggct gccatgcagc gcgctcgtct agacgagggt
     8521 cgcaagctcc cgcagaaagc cggcctgccc cgggagaatc cgacgcagtg cttcgtcgat
     8581 ggagaaccac tccacccgat cgatctcggg gaactcctgt tgtttccccg aacgcggcgg
     8641 ccattccatt ctgaacgtgt tgctgcgcat ctgcgccgcg tcgcaatcgc cctccacggc
     8701 ccaggcgtgc acgacctttc cgcccggctg tcgcagtagg cgcagtgccg tgaagttgcc
     8761 ggatacggtg aagccggtct cctcgtggaa ctcgcgccgg gccgcatcca atggcggctc
     8821 cggcgcgtcc acttccccct tggggatcga ccaggcgccg ctgtcccggt tggcccagaa
     8881 ggggccaccc gggtgtgcca gcaacacctc cagggtccct tgtcgcttcc ggtagacgag
     8941 gagtccggcg ctgaccttgg ccactctcag ggaaccccca cccgcgacgt cgcacccatt
     9001 tcgaagatcc ttccctcagg gggtccggct gcggggcacg gttcccgaaa tcagccgctc
     9061 gacggcgaac aggagcatga cgatcgaggc ccccaccgcg gcgcgcagcg ctcgcggtcc
     9121 ctgaccggac agggcgaagc gccaccagac ctgtccggcg aactgcacgt ggcgatacag
     9181 cagcgccccg atccacgctt cgaccaggaa ggccgcgccg gcggcgaacg cccatcggta
     9241 tcccatgggc tcacgcgtca acgaccccgg gcgcgggaac tcacgtcccg cgaggatgag
     9301 gacggcgagc gtgatcgcga ggaacaccgt caaccgcagg tcgaacgagc gcagcagggt
     9361 caggagaatg ccggcgccga acaacatgcg gaccatgtgg taggcgacgt gcgagcggcg
     9421 ctccagcccc caggcggaga gcacgagcac cgcgcccacg atgctgctca ggaaatggga
     9481 cgcctcgatc acgccgagcg gcagcagtcg gccgagccag gcgaggcggc ggtcgtcggc
     9541 cggaatggcg ccggtgacga ggaggaccag ccccgcgaga aacgtggtga aggcgagcac
     9601 acgcggcgcc agcgaggacg cgccgcgcaa cagttcccgc accgggtgct ccgaccggcg
     9661 cacccgctgt acggcacgcg cggcgagcaa cccccccgcg accgcaagcg gcaccaggta
     9721 gtagacgagg cggtacacca gcaacgcggc cagcgcgtcc gggacgggca cgctgctggg
     9781 tcgcagcagc agcacgaccg cctcgaatac cccgacccca ccgggaatcg ggaggaagag
     9841 tgtcgcgaac gcggccacga gaaacgcggt aaggaaggaa aagtgaggaa cggcggtgac
     9901 gccggcgagc aggatgaaca gcggtgcgga tgagaggagc caatccgcga ccgaaatccc
     9961 gagctgggcc agcgcgatcc gcagactggg gagcgcaaac gtgcggccca gcacgcgcac
    10021 ggccccatcc cccagccggg ccaggagcag ataggcggcg acgccggcga gcagcagcac
    10081 gccgagcgcc cggccgggca gcgcgaaggt atgggcgccc gctccggtgg gatcgatcag
    10141 cagcgccagg cccgcaaggc cggcgagccc gagcgcgtac gtcacgacgt tgaagccggc
    10201 gaccgtcacg gcgccggtcg ccgtcagccc gagcgtgcgg tacatgcggt agcgcacgcc
    10261 gccggcggtc accatggagg ccggggcgtt gttgctgacc gcgaagctca cgaacgaggg
    10321 gatcagcatc cggtgcagcg ggaggtccag tcccaggaac cggaaggcga cccagtcgta
    10381 gccgatgagc gcgaggtagc cgagcccgct gatcagcgcg gcgagcagga tgagggaaaa
    10441 ggggatcgcc tgcagcgcgg cgaagatgtc gcgcagcgga tagtcgtgca gcagccggtc
    10501 cagcgcccac agggccaggc cgaacagcag caggttcagc gcgggaacga cttccgcgcg
    10561 ccagcgggcg gtcgcgctca cctcaccgtt tctcgaaccg tccggtccgt tcgccgatca
    10621 cgatgcgata cactaccgcg gggcgcccgg tcacgtcggc gcggtgcacg atggaggggt
    10681 cgagaccgtg agaggggacg gccgtctcgc tcgtgaccag gggcagcagc cggtccatga
    10741 gcaggcccat cgcatgggct gcgcccgtgc cgcgcatcgt agacgtaggt gaccggcacg
    10801 acgtaagtgc ggccgccggc gtgacagccg aggcgtccca cgctttccga gcgcagtagc
    10861 cggtcgatct gtccatcggt cattgttccg agcatggtcc ctccggtctt cacaatcgca
    10921 tcattgcggg cgctatacga tggggcagga gagccggcga gcccccgtac cggagcggtt
    10981 atgacgcgca ggaaaatccc cgacgcgcct ggggccgcgc ccctcgaggc gcggattcca
    11041 ccccctgcgc atccccatcg gcgccaggtg ccgctgccct ggaaccagcc gaagtccccc
    11101 gcagacgatc ccgacgcgcc gcggcgcctg caggcgatcc tcgacagccc cggctaccgg
    11161 caggcggacc aggacgtgga gtttctcaat cgcgacgaga cgcgcggcct gcgcctgcaa
    11221 gtcgactatt tcaagccgga aatgctgctc gaggagcacg gcattcacga taccatcgtc
    11281 gttgtcggga gcacgaggat cctcgaaccc ggtgcggcgc gccggcaggt cgcgacgctg
    11341 cgcgaggcgc tggccggcaa cccctgcgac cgcgagctgg cgagccagct cgcggtggcg
    11401 gagcggatcc tggccaacag cgactactac gaggtggcgc gggagttcgg ccgtttggtg
    11461 ggggacggcg gcggtggggc ggtgcgcgcc gtgatcatga cgggcggcgg ccccgggatc
    11521 atggaagcgg cgaaccgggg ggcgttcgac gcgggagcca agtcgatcgg cttgaacatc
    11581 acgctgccgc atccgcagtt ccccaatccg tacatcactc cggaattgtg tttccgcttt
    11641 cactacttcg tcctgcgcaa gctccatttc ctgctgcgcg ccagggctct cgtggccttt
    11701 ccgggaggat acggcacctt cgacgaggtg ttcgaggcgc tcgccctggt gcagacgcgc
    11761 aaaatccggc cgtttcccat cgtgctggtg ggcgagcggt tctggcgccg cgccttcgac
    11821 atcgatttcc tggtcgagga gggcgtgatc gagccggaag accgcgacct gttctggttc
    11881 gccgaaaccg ccccggacat cctcggcggg atccggcact ggcacaccct caccgccgct
    11941 gagccgcagg tgtccgccgc gtaccggctc tagcctgtcc ggtcgcacac gagcggcgcg
    12001 gtcctggcgg gcccgcctgg tcgaccgctc agcgccggac ttcagcccac gagcgtggct
    12061 ccgaccgcac ctcgggaccg gccgcgacca gccgctgctg ggcgaccgcg cgcacgatgt
    12121 ccatgctcga cagcacgccg accagccggc cctcctccgt cacgatcagg cggtggacac
    12181 cggcccggac catgt
//
LOCUS       DBNGZW010000641         3987 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_264485, whole genome shotgun sequence.
ACCESSION   DBNGZW010000641 DBNGZW010000000
VERSION     DBNGZW010000641.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3987)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3987)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3987
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_264485"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..2699)
                     /locus_tag="AC16JC_15060"
     CDS             complement(207..2699)
                     /locus_tag="AC16JC_15060"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HIR5166067.1"
                     /translation="MRNVPRATLLIFGGAAAPLCAPSLPAQQPITDATRTLEKRDGFF
                     PLYWDAAKGRLLLEIPAGRLDQDFLYLPSLATGLGDAGTGLGLDRGTIGQEQIARFER
                     VGPRLLLVLQNPQFRALSDNPALVRSVNESFPRSTVAAFELQAEEGGRLLVDATSFVL
                     GDLTDVRGALRESQQGTFQIDRDRSTIFLPRTKAFPRNTEIEATLTFTSDQPGRAVQS
                     HAPEGRAITLRQHLSLVALPDSGFHPRRFDPRIGLFAVSFYDFAQPFDHDYVTRYAIR
                     HRLVKRDPGAAVSEPVQPIVYYLDAGIPEPYRTAFRQGAMWWNAVFAAAGFHNAFRVE
                     DMPADMDPMDARYNVIQWVHRTDPDYSIGPAFVDPRTGEIIKAAVRLESHRSVVDYDI
                     FAGTAPAVAGAEEADPLDMDEGLGDWIAGLDPRISAVAFAMARRRQHVAHEVGHTLGL
                     AHNFIAAADGRTSVMAYPAPLIRLVDGRIDLSQAYRDGPGAYDSLAIRWAYTQFPDGQ
                     EDSGLEAIVHEGTAHGLRFLTNPDEGDGNSYPEGTTWINGSDAVEELGRVQAVRDTLI
                     ARFDERAIQPGEPMALLSRRFATVYLHHRFTLGAAIKAVGGMEYRYGVRGDSLPVTRI
                     IPAARQRRALELLLDAIQPAELAIPERVLADLAPTPFGYSRDARGFRSPAAPAFDQLG
                     AARTLATQVIGGLLSTERTARIAALADRDPSLPTLTDVIGRIVARTWGTPSPARHPAL
                     LRVTQRVVVDQLCRLAGDPAATPESRAAAEWGLRRIARVLRAPAVADAETQAHRELAA
                     ADIERFLSRRDAPTKAPVAPPAPPLAPIGERH"
     gene            complement(2880..3770)
                     /locus_tag="AC16JC_15065"
     CDS             complement(2880..3770)
                     /locus_tag="AC16JC_15065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166068.1"
                     /translation="MRSLLRRGAVAVLVVAAGAVFAACTDHMSPAPPELSQADADSIG
                     AVVTADVENELDAGSAASGIGFVPGAPYSRALLSLSGNCTPDIAPQPVMNSDGDRVPD
                     SIRVTYTNCVIGFRRGADTVRGLIDIIDPTPQGTDGSLKVTFTDLVRIFVSRDGLRAS
                     VTQNGSRVAIRDTTTLSQTETGFRTDYVFGNGATATHLRDWSIVFTADLVGSIVRDAP
                     LPSGTLSIEGTSTITRNGSTTFDVHVSTGATPLHYNATCTDRPRFDQGTVVATVTRQG
                     TTSTVTIQFTACGQYTVTKS"
     gene            complement(3808..>3987)
                     /locus_tag="AC16JC_15070"
     CDS             complement(3808..>3987)
                     /locus_tag="AC16JC_15070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166069.1"
                     /translation="HGGGRVVWTAISPKAKRGYSSAALYQHENTLRLMAEGLGLTSFP
                     GTAANASNMAEFFTP"
BASE COUNT          575 a         1324 c         1504 g          584 t
ORIGIN      
        1 cccgttcggg gatggccagc tcggccggct ggatcgcgtc gagcagcagc tcgagcgcgc
       61 ggcgttggcg cgcggcgaca cggccgcggc cgtcgccggc tatcgcgacg cgctggcgaa
      121 ggactcgacc aacttggcgg cgaggagacg gctggcggcg ttggggcact agcagaggat
      181 ggtggaggac caccgtcatc caccgtctag tgtctctccc cgatcggcgc cagcggtggc
      241 gcgggcggtg cgaccggcgc tttcgtcggc gcgtcgcgcc gcgacaggaa ccgctcgatg
      301 tcggctgcgg ccagctcgcg atgcgcctgc gtctcggcgt ctgcgacggc cggcgcgcgc
      361 aggacccggg cgattcgccg cagcccccac tcggccgcgg cacgggactc cggagtcgcg
      421 gccgggtcgc cggcgagccg gcacagctga tccactacca cacgctgcgt cacgcgcaac
      481 agggccgggt gccgggccgg tgacggcgtc ccccaggtgc gtgccacgat ccggccgatc
      541 acgtccgtga gcgtcggcag ggaagggtcc cggtcggcca gcgccgcgat acgcgccgtc
      601 cgctcggtcg agagcagccc gccgatcacc tgggtcgcga gcgtgcgcgc cgcgccgagc
      661 tggtcgaagg ccggtgcggc gggcgatcgg aagccccggg cgtcgcgact gtagccgaag
      721 ggggtcggcg cgaggtcggc cagcacccgt tcggggatgg ccagctcggc cggctggatc
      781 gcgtcgagca gcagctcgag cgcgcggcgt tggcgcgcgg cgggaatgat ccgggtcacg
      841 ggcagcgagt caccgcgcac gccgtagcgg tactccatcc cgccgacggc cttgatggcc
      901 gcccccaacg tgaagcgatg atgcaggtag acggtggcga agcgccggct cagcagcgcc
      961 atcggctcgc ccggctggat cgcgcgctcg tcgaagcggg caatcagcgt gtcccgtacc
     1021 gcctgcaccc gacccagctc ttccaccgcg tcgctcccgt tgatccaggt cgtcccttcg
     1081 ggatacgagt tgccgtcgcc ctcgtcgggg ttggtgagaa agcgcagccc gtgcgccgtt
     1141 ccttcgtgca cgatcgcctc gagcccagag tcctcctggc cgtcggggaa ctgggtgtac
     1201 gcccagcgga tggcgaggga gtcgtaggcg cccggaccgt cgcggtaggc ctgggagagg
     1261 tcgatgcggc catcgacgag gcggatgagc ggggcggggt aggccatcac cgaggtgcgg
     1321 ccgtcggcgg cggcgatgaa gttgtgggcg agaccgagcg tgtgacccac ctcgtgggcg
     1381 acgtgctggc ggcggcgtgc catggcgaag gcgaccgcgc tgatgcgtgg gtcgaggccg
     1441 gcgatccagt cgcccagtcc ttcgtccatg tcgaggggat ccgcctcctc ggctcccgcc
     1501 acggcgggcg ccgtgccggc gaagatgtcg taatccacca cggagcggtg cgactccagc
     1561 cgcaccgccg ccttgatgat ctccccggtg cgcgggtcca cgaacgcggg gccgatcgag
     1621 tagtcggggt cggtgcggtg cacccactgg atcacgttgt agcgcgcgtc catcgggtcc
     1681 atgtccgcag gcatgtcctc gacgcgaaag gcgttgtgga agcccgccgc tgcgaacacg
     1741 gcgttccacc acatcgctcc ctggcggaac gcggtccggt agggctcggg gatgcccgca
     1801 tcgagatagt agacgatcgg ctgcaccggc tccgagacgg ccgcgccggg gtcccgcttc
     1861 accaggcggt gccggatcgc gtagcgcgtg acgtaatcgt ggtcgaacgg ctgcgcgaag
     1921 tcgtagaagg acacggcgaa caggccgatg cgcgggtcga atcgccgcgg gtggaacccc
     1981 gaatcgggca gggcgaccag cgagagatgc tggcgcaacg tgatggcgcg cccctcgggg
     2041 gcgtgcgact ggacggcgcg ccccggctgg tcgctcgtga acgtgagggt ggcctcgatc
     2101 tccgtgttgc gcgggaacgc cttggtgcgg ggcaggaaga tcgtgctgcg gtcgcgatcg
     2161 atctggaacg tgccctgctg ggactcgcgc agcgcgccgc gcacgtcggt gaggtcgccc
     2221 agcacgaacg acgtcgcgtc caccaacagc cggccgccct cctcggcttg gagctcgaac
     2281 gccgccaccg tggagcgggg gaacgactcg ttcaccgagc gcaccagcgc cgggttgtcg
     2341 ctgagcgccc ggaactgcgg attctggagc accagcagca ggcgggggcc gacccgctcg
     2401 aagcgggcga tctgctcctg cccgatggtg ccgcggtcga ggcccagccc ggtgcccgcg
     2461 tcccccaggc cagtcgcgag ggagggcagg tagaggaaat cctggtccag gcgcccggcg
     2521 gggatctcga gcagcagccg ccccttggcg gcgtcccagt agagcgggaa gaagccgtcg
     2581 cgtttctcga gggtgcgggt ggcgtcggtg atcggctgct gcgcggggag cgacggggcg
     2641 cacagcggcg cggccgcgcc tccgaaaatc aggagcgtcg cacgaggaac gtttcgcatg
     2701 gggctttcgt ctccgaggct aggaagcctg cacggcccgg acggccatcg cgcctccgtc
     2761 gtgctcggtg atctcctcga atgctggacg ctgacccggc tcgcgccagc cttcgggtga
     2821 cgtcatccgc gcgggctcca acagggcccg gatgcgccac gcgtggaatg gcgtcgcgct
     2881 tacgacttgg tgaccgtgta ctgcccgcag gccgtgaact ggatcgtgac ggtggacgtt
     2941 gtgccctgtc tcgtgaccgt ggcgacgacc gtgccctggt cgaatctggg ccggtcggtg
     3001 cacgtggcgt tgtagtgcag gggcgtggcc cccgtggata cgtggacgtc gaaggtggtg
     3061 ctgccgttcc gcgtaatggt cgacgtgccc tcgatggaca gcgtcccgct gggcagcggc
     3121 gcgtcccgga cgatcgagcc gaccaagtcc gccgtgaaga cgatcgacca gtcccggagg
     3181 tgggtggcgg tcgcgccgtt gccgaacaca tagtcggtgc ggaatcccgt ctccgtctgc
     3241 gacagggtcg tcgtgtcgcg gatcgcgacc cggctgccgt tctgggtgac cgacgcccgg
     3301 agcccgtcgc ggcttacgaa gatcctcacg agatcggtga acgtgacctt gagcgagccg
     3361 tcggtgccct gcggcgtcgg gtcgatgatg tcgatcaggc cgcgcacggt atccgcgccg
     3421 cggcggaagc cgatcacgca gttggtgtac gtgacccgga tcgaatcggg gacgcgatcg
     3481 ccgtcggaat tcattaccgg ctgcggcgcg atgtccggtg tgcagttgcc cgagagcgac
     3541 agcagggccc tggaatacgg cgcacccggc acgaagccga tgccgctggc ggccgagccg
     3601 gcgtcgagct cgttctcgac gtcggccgtg accaccgccc cgatgctgtc ggcgtccgcc
     3661 tgggagagct ccggcggcgc cggggacatg tgatcggtgc aggccgcaaa cacggcgccg
     3721 gcggccacaa ccaggaccgc caccgcgccg cgacgcagga gggacctcat gagggcggac
     3781 tccagagtgg ggacgagggc ataggactca gggcgtgaag aattcggcca tgttggacgc
     3841 gttcgccgcc gttccgggga agctggtcaa acccagtcct tcggccatga gccgcagtgt
     3901 gttctcatgc tggtacaacg cggccgacga gtagccgcgc ttcgccttgg ggctgatcgc
     3961 ggtccagacg acgcggccgc cgccatg
//
LOCUS       DBNGZW010000642         2841 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1629747, whole genome shotgun sequence.
ACCESSION   DBNGZW010000642 DBNGZW010000000
VERSION     DBNGZW010000642.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1629747"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..478
                     /locus_tag="AC16JC_15075"
     CDS             <1..478
                     /locus_tag="AC16JC_15075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166070.1"
                     /translation="SFAEAVATTVADIARAIGGRTLALFTAHAALRQVAALLEPGGML
                     IATVPAFRLLWTTHDVLNHHFTRYTRRGFTALARSAGLDIVRARYFFHWVFPAKLGVR
                     LLESVRRPRPAPPRVPPSWINEPLYLLSRLEQRLVTPLGPPFGSSLLAVAHASARG"
     gene            522..1241
                     /locus_tag="AC16JC_15080"
     CDS             522..1241
                     /locus_tag="AC16JC_15080"
                     /inference="COORDINATES: protein motif:HMM:NF012579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR5166071.1"
                     /translation="MQLTVHQAASYLGVDESTVRRWISLRGLPAHRVNERLHLNAIEL
                     WEWATENGIAVSRRLLDQARRRPDEVPPLSQLLATGGIHHDVGGEGKAGVLREIVGHL
                     PLPDDTDRQFLVTVLEAREAMGSTGIGNGIAIPHVRNHIVLHVEAPFVTLCLLRHPVD
                     FDAVDGRPVHALFTLISPSVPAHLRILAQLGFVLRDEELRTLLARRAPAPELLARVRL
                     LEQQTSGMHEAAPRRARPLGS"
     gene            1238..>2841
                     /locus_tag="AC16JC_15085"
     CDS             1238..>2841
                     /locus_tag="AC16JC_15085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012098588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR5166072.1"
                     /translation="MTLFFLGIAAIVAASVSAAALHRRPVVAGHAFTALVSAGCVLGA
                     IPAVHVLAGGPALQGRLGTWDFGIDLLSAAFLLVVLGPGLASAWYGNRALGDERDRPV
                     GAARTLFALELAALALVVTARGVVPFLVAWEVMAVLAYFLVIFEHERAEVRRAGLVYL
                     VATHAGTLALIALFSFWAGPSGALSFDALAARTAALPAGGALVLWLALLGFGLKAGIV
                     PLHFWLPDAHASAPSHVSALMSGVVIKMGIYGLFRIVGLLGAPPAWWGWLVLALGIGS
                     GVLGVLWALAQHDLKRLLAYHSVENIGIILMGLGAGALGMSYGIPLVATLGFAGAMLH
                     TLNHALFKSLLFLGAGATVHATGTRSLDRLGGLARRMPLTWVAFLVASAAIVGLPPLN
                     GFVSEWLVYQALLGGGTGGGPIRLTLIGVAALALIGGLALACFAKVCGIVFLGTPRSA
                     DAEHGHEAPRGLLGPMFGLVAGCVLIGLVPALAVRPALAVGAAVAQVPVTAAGTGVIA
                     ALTPIALVSIGLLVLLWIGSVAMAAVR"
BASE COUNT          297 a         1020 c         1027 g          497 t
ORIGIN      
        1 gagcttcgcc gaggccgtcg ccacgacggt cgcggacatc gcgcgtgcca tcggcgggcg
       61 gacgctggcg ctgttcaccg cgcacgcggc gctgcggcag gtcgcggcgc tgctcgagcc
      121 cggcgggatg ctcattgcga cggttccggc gttccgactc ctctggacga cgcacgacgt
      181 gctgaaccac cacttcacgc gctatacccg gcgcggtttc acggcgctgg cccgctcggc
      241 cggcctcgac atcgtgcgcg cgcgctactt cttccactgg gtttttccgg cgaagctcgg
      301 cgtgcggctg ctggaatccg tgcggcgtcc ccggcccgcc ccgccgcggg tcccgccatc
      361 ctggatcaac gagccgctct acctgctctc gcgtctggag cagcggctcg tcacgccgct
      421 cgggccgccg ttcggcagct cactgctcgc cgtggcgcac gcttccgcgc gcggttagct
      481 tcgcgctcgg tgtccgctcc acacctccac ttcgctcccg catgcagctg accgtccacc
      541 aggcggcctc ctacctcggc gtcgacgagt cgaccgtgcg ccgctggatc tcgctgcgcg
      601 ggctgccggc ccaccgggtc aacgagcgcc tgcacctgaa cgccatcgag ctgtgggagt
      661 gggcgacgga gaacggcatc gccgtctccc gccggctgct cgaccaggcg cggcggcggc
      721 cggacgaggt gccgccactc tcccagctgc tggccaccgg cgggatccat cacgacgtgg
      781 gtggtgaagg gaaggcgggg gtgctgcgcg agatcgtcgg gcacctgccg ctgccggacg
      841 acaccgatcg ccagttcctc gtcacggtgc tcgaggcgcg cgaggcgatg ggctccaccg
      901 gcatcggcaa tggcatcgcg atccctcacg tacgcaacca catcgtgctg cacgtcgagg
      961 cgccgttcgt gacgctgtgc ctgctgcgtc atccggtgga cttcgatgcg gtagacggca
     1021 ggccggtgca cgccctgttc acgctgatca gccccagcgt accggcgcac ctgcgcatcc
     1081 tcgcccagct gggcttcgtg ctccgggacg aggagctgcg cacgctgctg gcgcggcgcg
     1141 cgccggcccc ggagctgctc gcccgggtgc gcctgctgga gcagcagacg agtggaatgc
     1201 acgaagccgc gccgcggcgg gcacgcccgc taggctcgtg acgctgttct tcctgggcat
     1261 cgccgcgatc gtggccgcga gcgtgagcgc cgccgcgctc caccgccgtc ccgtcgtcgc
     1321 gggtcacgcg ttcacggcgc tggtgagtgc cggctgcgtg ctgggagcca tcccggcagt
     1381 gcacgtgctg gccggtgggc cggcgctgca gggccggctc gggacctggg acttcgggat
     1441 cgatctcctc tccgctgcgt ttcttttagt cgtgctcggg ccgggactag ccagtgcctg
     1501 gtacgggaac cgcgcgctgg gcgacgagcg cgaccggccg gtgggcgcgg cccggaccct
     1561 cttcgcgctc gagctggccg ccctcgccct ggtggtcacc gcgcgcggcg tggtgccgtt
     1621 cctcgtggcc tgggaagtga tggcggtgct cgcctacttt ctggtcatct tcgagcacga
     1681 gcgcgccgag gtgcggcgcg cgggactcgt gtatctcgtc gcgactcacg ccggcaccct
     1741 cgcgctgatc gcgctgttct ccttctgggc cggcccgagc ggcgcgctga gcttcgacgc
     1801 gctggcggcg cgcacggccg cgctgcccgc cggcggtgct ctcgtgctgt ggctcgcgct
     1861 cctggggttc gggctcaagg ccggtatcgt gccgctgcac ttctggctgc cggacgcgca
     1921 cgcgtcggcc ccatcgcacg tctcggcgct gatgtcgggt gtcgtgatca agatggggat
     1981 ctacggtttg ttccggatcg tcggtctgct cggcgcaccg cccgcgtggt ggggctggct
     2041 ggtgctcgcc ctcggcatcg gctccggcgt gctcggcgtg ttgtgggcgc tggcgcaaca
     2101 cgacctgaag cggctgctcg cgtatcacag cgtcgagaac atcggcatca tcctcatggg
     2161 cctgggcgcg ggggcgctgg ggatgagtta cgggattccg ctcgtcgcca cgctggggtt
     2221 cgccggcgcg atgctccaca cgctgaacca cgcgctcttc aagagtctcc tctttctcgg
     2281 ggccggggcg acggttcacg ccaccgggac ccggtcgctc gaccggctgg gcggcctcgc
     2341 gcgtcgcatg ccgctgacct gggtcgcgtt cctcgttgcg tccgccgcca tcgtgggcct
     2401 cccgccgctc aacgggttcg tgagcgagtg gctggtctat caggcgctgc tgggcggggg
     2461 aacggggggg ggaccgatcc ggctcacgtt gatcggcgtc gcggcgctcg cgctgatcgg
     2521 cgggctcgcg ctcgcctgct tcgcgaaggt ctgcggcatc gtgtttcttg gaacgccccg
     2581 cagcgccgac gcggagcacg gtcacgaagc cccccgcggt ctgctgggcc cgatgttcgg
     2641 cctcgtcgcg ggttgcgtcc tgatcgggct cgttcccgcg ctcgccgtgc ggccggcgct
     2701 ggccgtgggc gcggcagtcg cccaggtccc agtgaccgct gccgggaccg gcgtgatcgc
     2761 ggccctcacc ccgatcgccc tggtgtcgat cgggttgctg gtactgctgt ggatcggcag
     2821 cgtggcgatg gctgccgtgc g
//
LOCUS       DBNGZW010000643         4092 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_727695, whole genome shotgun sequence.
ACCESSION   DBNGZW010000643 DBNGZW010000000
VERSION     DBNGZW010000643.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4092)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4092)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4092
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_727695"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..832)
                     /locus_tag="AC16JC_15090"
     CDS             complement(<1..832)
                     /locus_tag="AC16JC_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013909829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR5166073.1"
                     /translation="MPRPFDLPPQYDPGAVEAGLYRTWTERGAFAPVPGPGREPYVIV
                     IPPPNVTGVLHMGHGLNNTIQDVLIRFERARGREALWLPGTDHAGIATQNVVERLIAK
                     EGNTRFDLGREAFVARVWEFVRATGSTILEQLKQIGASCDWTRTRFTLDPAYTRAVRE
                     VFVHLWERGLIYRGHRVIHWCPRCLTALSDEEAEFRDTSGTLYYINYPLAEGSGYVTV
                     ATTRPETLLGDTAVVVHPEDPRGAPFIGKHVRLPITGIDIPVLADEAVEKEFGTGFVK
                     V"
     gene            960..1124
                     /locus_tag="AC16JC_15095"
     CDS             960..1124
                     /locus_tag="AC16JC_15095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166074.1"
                     /translation="MPEDPSVVPSSQSRALAILLLILVLTGVALYFRFGTRLPAFGSV
                     EPTSAADTSR"
     gene            1127..3022
                     /gene="thrS"
                     /locus_tag="AC16JC_15100"
     CDS             1127..3022
                     /gene="thrS"
                     /locus_tag="AC16JC_15100"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR5166075.1"
                     /translation="MPATAIRVTLPDGSTRELPASATGADLARSIGPGLAKAAVAVRV
                     NGEVRDLARPLADGAAVAILTDRDPQALDVLRHSSAHVLATAVRELFPHARIGFGPAI
                     EDGFYYDFEVPAPFTPEDLEKIEARMRDVAAADYPFVREEVSRDEAKHRFAEDPLKLE
                     RIDDLGPNEVISVYTDGPFVDLCRGPHLASTGKLKHFKLLHAAGAYWRGDERRQMLQR
                     IYGTAWFSKDDLEAYLRRLEEARKRDHRVLGKQLDLFSIQEDAGPGLIFWHPKGAMLQ
                     FQLRRFVEDAILARGYELVYTPHITREQLFVRSGHLPYYEENQFPAMAAAAGDAESVR
                     YRVKPMNCPMHILVYKSQQRSYRDLPIRLAEIANVYRNERSGTLHGMLRVRGLTMDDG
                     HLFLRADQIEDEIATCVDLVDYVFRRTFGIEYRLDLATRPPERLGDDAIWDRAEAALK
                     GALERLKLTYRLDEGGGAFYGPKIDVKFNDAIGREWQGATIQLDFNLPERFALEYTGE
                     DNRPHRPVMIHRAIFGTMERFVGFLIEHFAGAFPLWLAPEQVRVLSIADAQLEAARGV
                     AETLRRAGLRAHLDERSDTLNYKIRDGELQKIPYLAVVGQREADAGAVAMRALGSRQE
                     ADRVRDR"
     gene            3180..>4092
                     /locus_tag="AC16JC_15105"
     CDS             3180..>4092
                     /locus_tag="AC16JC_15105"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR5166076.1"
                     /translation="MATDQTTPVIISACRTPIGRFLGGLSPLTAPKLGALVVREAVRR
                     AGIEAGAVEEVILGNVLQGGEGQAPARQAAIHAGLPGTIPSLTINKVCGSGLKAVMLA
                     AQAVKAGDAQCLVAGGMESMSNAPFYLYGMRGGVKAGNQPLTDGMIHDGLWDSFSNQH
                     MGNLAEYTAKKAGVSRADQDAFALASHRKAVAAMEACHFKAETVAVEIAGKKGPTVID
                     KDEGPRADTSLEALGALKPSFEKDGTVTAGNAPGLNDGASALVVTSLAFARAHGLQPL
                     ARITAYATGGGEPKDLFFAPIVAVQNLM"
BASE COUNT          656 a         1368 c         1443 g          625 t
ORIGIN      
        1 tcaccttgac gaatccggtg ccgaactcct tctccaccgc ctcgtcggcg agcaccggga
       61 tgtcgatgcc cgtgatcggc agccgcacgt gcttgccaat gaaaggggcg ccacggggat
      121 cctcgggatg caccaccacg gccgtgtcgc ccagcagcgt ctcgggacgc gtcgtggcta
      181 ccgtgacgta gccgctcccc tcggccaggg gatagttgat gtagtagagg gtgccgctcg
      241 tgtcgcggaa ctccgcctcc tcgtcggaga gggcggtgag gcagcgcggg caccagtgga
      301 tcacccggtg gccgcggtag atcagccccc gctcccacag gtgcacgaac acctcgcgca
      361 cggcgcgcgt gtaggcggga tcgagcgtga accgggtgcg ggtccaatcg cacgacgcgc
      421 cgatctgctt cagctgctcg aggatcgtgc tgccggtggc gcgcacgaac tcccacacgc
      481 gcgcgacgaa cgcctcgcgc cccagatcga accgggtatt gccttccttg gcgatgaggc
      541 gctcgaccac gttctgcgtg gcgatgccgg cgtggtccgt cccgggaagc cacagcgcct
      601 cccgcccgcg ggcccgctcg aaccggatca acacgtcctg aatcgtgttg ttcaggccgt
      661 gacccatgtg cagcaccccg gtcacgttgg gcggcgggat gacgatgacg tagggctcac
      721 gccccgggcc ggggaccggg gcgaaggcgc cgcgctccgt ccaggtgcgg tacaggcccg
      781 cctcgacggc gccggggtcg tactgcgggg ggaggtcgaa cggtcgcggc atggctacaa
      841 gatacggggg gacgagggag gagggacggg ggacgaggcc catccccacc cgtccccctc
      901 ccgcgtcgct tacgcgtccc ccatccctcg tccctcgtcc ccttgtgtta aaattcgcca
      961 tgcctgagga tccttccgtc gtcccgtcga gccagtcgcg cgcgctggca atcctgctgc
     1021 tgatcctggt gctgaccggc gtggcgctct acttccgttt cggaacccgg ctcccggcct
     1081 tcggctcggt cgagcccacc agcgccgccg acacgtcccg ctgaccatgc cggccaccgc
     1141 gattcgcgtc accctccccg acgggagcac cagggaactg cccgcctcgg ccaccggggc
     1201 cgatctcgcc cggagcatcg gcccgggact cgccaaggcc gccgtcgccg tgcgcgtcaa
     1261 cggggaagtc cgtgatctgg cccggccgct tgcggacggc gccgcggtgg ccattctcac
     1321 cgaccgcgac ccgcaggcgc tggacgtgct gcgccattcc tcggcgcacg tgctggccac
     1381 ggcggtacgg gagctgttcc cgcacgcgcg aatcgggttc gggccggcga tcgaggacgg
     1441 cttctactac gacttcgagg tgccggcgcc gttcaccccg gaggacctcg agaagatcga
     1501 agcgcgcatg cgggacgtgg ccgcggccga ctacccgttc gtgcgcgagg aggtgagccg
     1561 ggacgaggcg aagcatcgct tcgccgagga tccgctgaag ctcgagcgca tcgacgacct
     1621 ggggccgaac gaggtgatct cggtgtacac cgacggtccg ttcgtcgatt tgtgccgcgg
     1681 gccgcacctc gcgagcacgg ggaagctcaa gcacttcaag ctgctgcacg cggcgggggc
     1741 gtactggcgc ggcgacgagc ggcgccagat gctgcagcgc atctacggca cggcctggtt
     1801 ctccaaggac gatctcgagg cctacctgcg gcggctggag gaggcccgca agcgcgacca
     1861 ccgcgtgctg ggcaagcagc tcgacctctt ctcgatccag gaggacgcgg ggccggggct
     1921 gatcttctgg catcccaaag gggcgatgct gcagttccag ctgcgccgct tcgtggagga
     1981 cgcgatcctc gcgcgtggct acgagctcgt gtacaccccc cacatcacgc gcgagcagct
     2041 gttcgtccgt tccgggcacc tgccgtacta cgaggagaac cagttcccgg ccatggccgc
     2101 ggcggcggga gacgccgaga gcgtgcgcta tcgcgtcaaa ccgatgaact gcccgatgca
     2161 catcctggtg tacaagtcgc agcagcgcag ctatcgcgac ctccccatcc gcctcgccga
     2221 gatcgcgaac gtgtatcgta acgaacgctc ggggacgctg cacgggatgc tgcgcgtgcg
     2281 gggcctgacg atggacgacg ggcacctgtt cctgcgcgcg gaccagatcg aggacgagat
     2341 cgcgacctgc gtggacctgg tggactacgt gttccggagg accttcggca tcgagtaccg
     2401 gctcgatctc gcgacgcggc cgcccgagcg gctgggcgac gatgcgatct gggatcgcgc
     2461 ggaggcggcg ttgaagggag cgctggagcg cctgaagctg acctaccggc tggacgaggg
     2521 gggcggggcg ttctacgggc cgaagatcga cgtcaagttc aacgacgcca tcggccgcga
     2581 atggcagggg gcgacgatcc agctcgattt caacctgccc gagcgcttcg cgctggagta
     2641 cacgggcgag gacaaccggc cgcaccgccc ggtgatgatc caccgggcga tcttcggcac
     2701 gatggagcgc ttcgtcggct tcctcatcga gcatttcgcc ggcgcgtttc cactgtggct
     2761 cgccccggag caggtgcgcg tcctgtcgat cgccgacgcg cagctcgagg ccgcgcgcgg
     2821 cgtggccgag acgctgcgcc gcgcgggcct gcgggcccac ctcgacgagc gcagcgacac
     2881 cctgaactac aagatccgcg acggggaatt gcagaagatc ccctatctcg cggtcgtggg
     2941 gcagcgggaa gccgatgccg gtgcggtggc gatgcgggcc ctggggagcc ggcaagaagc
     3001 agaccgtgtt agggatagat gaagcggtgc ggctcctgac cgaggaggtt cgcacgcgct
     3061 ccgtcacgcc gttaatatga attgcggatt gtggatttcg gattgcggat tggagaactg
     3121 gaaacggccc gtacaatccg caatcaacaa tccgccatcc gcaatctgag gaacggatca
     3181 tggctaccga tcaaactact ccagttatca tctccgcctg tcgcacgccg atcggccggt
     3241 tcctgggcgg cctgtctccc ctcacggcgc cgaagctcgg cgcgctggtg gtgcgggagg
     3301 ccgtgcgccg cgccggcatc gaggccggag cggtggagga ggtcatcctg ggcaacgtgc
     3361 tgcagggcgg cgaggggcag gccccggcgc ggcaggcggc gatccatgcg gggcttcccg
     3421 ggacgatccc gagcctcacc atcaacaagg tgtgcggctc cgggctcaag gccgtgatgc
     3481 tggcggcgca ggcggtgaag gccggcgacg cgcagtgcct ggtggcgggt gggatggaat
     3541 cgatgtcgaa cgctcctttc tatctgtatg ggatgcgggg tggggtgaag gcgggcaacc
     3601 agccgctcac cgacggcatg atccacgacg gtctgtggga ctcgttctcg aatcagcaca
     3661 tggggaatct ggccgagtac acggcgaaga aggccggtgt ctcgcgcgcc gaccaggacg
     3721 ccttcgccct ggcgagccac cgcaaggcgg tggccgcgat ggaggcctgt cacttcaagg
     3781 ccgaaacggt ggccgtggag atcgccggga agaagggacc caccgtcatc gacaaggacg
     3841 agggcccgcg cgccgatacg agcctcgagg cgctcggtgc gctcaagccg tcgttcgaga
     3901 aggacggcac cgtgacggcc ggcaacgcgc cgggattgaa cgatggggcg tccgcgttgg
     3961 tcgtgacgtc gctcgccttc gcccgggcgc atggcctcca gccgctggcg cgcatcaccg
     4021 cctacgccac cgggggcgga gagccgaagg atctcttctt cgcaccgatc gtcgcggtcc
     4081 agaacctgat gc
//
LOCUS       DBNGZW010000644         2049 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1067792, whole genome shotgun sequence.
ACCESSION   DBNGZW010000644 DBNGZW010000000
VERSION     DBNGZW010000644.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1067792"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..237
                     /locus_tag="AC16JC_15110"
     CDS             <1..237
                     /locus_tag="AC16JC_15110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166077.1"
                     /translation="CTGAMCPVCAVSRDAMGARLAKSGLRPEVRRKVVSICDGCGQET
                     ELVVSSGGYVSCTECGSTWRWRVRERGEEEWDWA"
     gene            complement(317..>2049)
                     /locus_tag="AC16JC_15115"
     CDS             complement(317..>2049)
                     /locus_tag="AC16JC_15115"
                     /inference="COORDINATES: protein motif:HMM:NF012343.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR5166078.1"
                     /translation="GSLVPSANDPFSPDAPAPLAQASYPTAAASTAEMTSSERRLVLG
                     NMWVAIIAFGVAAVMAVMQAMSRANINVPNHTPRMYYMSITAHGVLMALVFTTFFIMG
                     FGYAIVHREAGRIRWPGLSKATFWLAVVGTLTAAIPILTFKASVLYTFYPPLQAHPAF
                     YIGLTLVVVGSWGWAANVVATWWSLRNSDPTRPVSLAMHATTANAMLWLLCTLGVASE
                     MLFQLIPWSLGLKATVDPLLARTLFWFTGHPLVYFWILPAYAVWYTVLPRESGGYLFS
                     EPLGRMVFAYFLVISTPVGLHHQFQDPGIPASWKLFHTLLTMAISFPSLVTAFTICAS
                     LEVAGRLRGGTGLFGWIRRLPWGNPVVSSVLYAMLLFLVGGFGGAVNAAYALNSVVHN
                     TAWIHGHFHTTVGSAAALSFMGAMYWLIPRLTGRELSFRTSFARWQPLLWFVGMLFFS
                     IPSHIAGLMGLPRRVFATDYGGAVQAMQWQGLTMTSAIGGIILFVSAMFFVGVIVHTL
                     LMGRRTTKAPLVWADSLGMPAEGFRATIWDRLGMWWAIAGVLVLVAYAIPLYDILTHA
                     RYGSPGFKPF"
BASE COUNT          382 a          662 c          740 g          265 t
ORIGIN      
        1 tgcaccggcg cgatgtgccc ggtgtgcgcg gtgtcgcgcg acgcgatggg cgcgcgcctc
       61 gcgaagagcg ggctccgccc ggaagtgcgc cgcaaggtag tctcgatctg cgacggctgc
      121 ggccaggaga ccgagctcgt ggtctcatcg ggcggctacg tgagctgcac ggaatgcggc
      181 tcgacgtggc gctggcgggt gcgggagcgg ggggaggagg agtgggattg ggcatgaggg
      241 cggtagtggg tggtgggtga tgggcggtgc gtgacgccgc cacccatcac ccatcaacca
      301 tcacttacca cgcgccctag aacggcttga accccgggct cccgtacctt gcgtgcgtga
      361 ggatgtcgta gagcgggatc gcgtaggcca cgagcaccag gacgcccgcg atcgcccacc
      421 acatgccgag gcggtcccag atcgtcgcgc ggaagccctc ggcgggcatg ccgagcgagt
      481 cggcccacac cagcggcgcc ttcgtcgtgc ggcgccccat gagcagcgtg tgcacgatca
      541 cgccgacgaa gaacatcgcg ctcacgaaca ggatgatgcc gccgatggcc gaggtcatcg
      601 tgagtccctg ccactgcatc gcctgcacgg cgccgccgta atcggtcgcg aacacgcggc
      661 gcggcagtcc catcaggccg gcgatgtggc tcgggatgga gaagaacagc atcccgacga
      721 accagagcag gggctgccag cgggcgaacg acgtgcggaa cgacagctcg cgtccggtga
      781 ggcgcgggat gagccaatac atcgcgccca tgaacgagag cgccgccgcg ctgccgaccg
      841 tcgtgtggaa gtgaccatga atccacgccg tgttgtgcac gaccgagttg agcgcgtacg
      901 ccgcgttcac cgcgccgccg aatccgccga cgaggaagag gagcatcgcg tacagcacgc
      961 tcgacacgac gggattcccc cagggcagcc ggcgaatcca gccgaacagg cccgtccctc
     1021 cccgcagccg gcccgccacc tcgagcgacg cgcagatcgt gaacgcggtg acgaggctcg
     1081 ggaaggagat ggccatcgtg agcagggtgt ggaacagctt ccaactcgcc gggatgccgg
     1141 gatcctggaa ctggtggtgc agccccaccg gcgtcgagat gacgagaaag taggcgaaca
     1201 ccatccggcc gagcggctcc gagaacagat agccgccgga ctcgcgcggc agcacggtat
     1261 accacacggc gtacgccggc aggatccaga agtagacgag cggatggccg gtgaaccaga
     1321 acagcgtgcg cgcgagcagt ggatcgaccg tcgccttgag gccgagcgac caggggatga
     1381 gctggaacag catctccgag gcgacgccga gcgtgcagag gagccagagc atcgcgttcg
     1441 cggtggtggc gtgcatcgcg aggctgaccg ggcgcgtggg atcgctgttg cgcagcgacc
     1501 accacgtcgc cacgacgttc gccgcccacc cccacgaccc cacgacgacg agcgtgagcc
     1561 cgatgtagaa cgccgggtgc gcctggagcg gcggatagaa ggtgtagaga acgctcgcct
     1621 tgaaggtgag aatcgggatc gcggccgtga gcgtgccgac cacggcgagc cagaaggtcg
     1681 ccttcgagag gccgggccag cggatgcgcc ccgcctcgcg gtgcacgatg gcgtagccga
     1741 accccatgat gaagaaggtc gtgaacacga gggccatcag cacgccgtgc gccgtgatcg
     1801 acatgtagta catccgcggc gtgtggttcg gcacgttgat gttcgcgcgg ctcatcgcct
     1861 gcatcaccgc catcaccgcg gcgacaccga aggcgatgat cgccacccac atgttgccga
     1921 gcacgagccg ccgctcggac gacgtcatct cggcggtgct cgccgccgcg gtgggatagc
     1981 tcgcctgcgc cagcggcgcg ggtgcgtcgg gcgagaaggg atcattcgcg ctcggcacca
     2041 gggagccga
//
LOCUS       DBNGZW010000645         3728 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1287738, whole genome shotgun sequence.
ACCESSION   DBNGZW010000645 DBNGZW010000000
VERSION     DBNGZW010000645.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3728)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3728)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3728
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1287738"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..863)
                     /locus_tag="AC16JC_15120"
     CDS             complement(<1..863)
                     /locus_tag="AC16JC_15120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166079.1"
                     /translation="MTADVIIEFRRRPSVLAYMLRGLYPSPGVHGTGGGPAIRARWRS
                     HRVDRRHLAEFLQLSGLRADAGMPILFPHVFSFPLQMAVLTHPRFPMPFWKVLQIRNH
                     QLQHRSIFADTVLDLETRVVGQRILEKGVEFDLHTSISARQELVWESLNTFYYRGRYG
                     QTGAASPLALAPRHGDTLVARWRTAAAASGWRFAALTGDYNGIHYWHWYARRLGFRRA
                     FLPPQLVLGQCMARLSAPRATERQRLDVWLKGPVYTDAAVRLNATTAVDRTVFGVSLD
                     GEERPAILASWR"
     gene            complement(853..1299)
                     /locus_tag="AC16JC_15125"
     CDS             complement(853..1299)
                     /locus_tag="AC16JC_15125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166080.1"
                     /translation="MIIRFKEQFALPVGVVFGYFATPADWVRLYGFGGTVRELGDGWY
                     AVPLKRFPFPLVAKITARQENTLVRWTYRGFWRGEGEIRFTQDNGIVMVEGHEAVAVR
                     WLFRASPLLERWFLDRPFRRLWESGWRRLRKLEAQERRAEPRNHDR"
     gene            complement(1337..1771)
                     /locus_tag="AC16JC_15130"
     CDS             complement(1337..1771)
                     /locus_tag="AC16JC_15130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166081.1"
                     /translation="MDADRGFRLLRAAFIAGAVTDALAVIPMLSPSVATALWGFQDPG
                     GPYLLAMGSAAALMLGWTGLLVWAWQRPLERRFIAALTAVVIGGLAVTEIVGVLAAWV
                     ALWRVVPTLILQTVLLALFGVAYLASAPHSADSKRQVTTDVR"
     gene            complement(1780..3135)
                     /locus_tag="AC16JC_15135"
     CDS             complement(1780..3135)
                     /locus_tag="AC16JC_15135"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR5166082.1"
                     /translation="MKSLATFPWIAGVACLLALHAGPAAAQQPVTLTLGGAARFAAER
                     SAGPEAARYRVDMAEARLRQQHADLLPTVAGVLSQGERTLNSAGFGLALRDPTTGSDL
                     FDPAGQVLGPVRTWDLRATARQSVVDFSSFARVRAARATVAAADADATNESQQAAASA
                     AMLYVRALSADAQLRARQADSTLAEELLGIARNQRDAGMGIALDVTRAQAQLAATRAQ
                     LIAARTEHERARLQLHRALGLPFDAPLTLADSLLEMPTALPAPLEAETNERALRTRAD
                     LRMIGVQMDGVERQIAAIKAERLPALAIFADWGSTGNSTARLLNTYSWGVQVSIPVFD
                     GLRREGRIAEQRSAMRELDVRRRDLVQQVMLEVRVALLELTSAAEQIRATDERLALAE
                     QELELARRRFTQGVAGNADVITALLGLNAARTQSVDARATFQSARVALALAQGVVTEL
                     P"
     gene            complement(3169..3612)
                     /locus_tag="AC16JC_15140"
     CDS             complement(3169..3612)
                     /locus_tag="AC16JC_15140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166083.1"
                     /translation="MTITLASITHAYEFGTPAFVVGAIVIAILVALSSHYRRTGNRLV
                     LVLYGLLNLWVIGGFGLVGGLWNHTVKVAVSAVNDGALPLSLQRFFMSPELGSAPYEA
                     IGFLTFVASMFAAYFGYRFARTVRWMGRADSPGQGAVRSSPGVRV"
BASE COUNT          651 a         1294 c         1236 g          547 t
ORIGIN      
        1 cgccagctgg cgagaatcgc cggtcgttcc tcgccatcca gggacacccc gaagacggtt
       61 cggtccactg ccgttgtggc gttgagtcgc accgccgcgt cggtatacac agggcccttc
      121 agccacacgt ccagtcgctg gcgctccgtc gctcgaggcg cgctcaggcg cgccatgcac
      181 tgcccgagca cgagttgcgg cggcaggaac gcccgccgga agccgagccg tcgggcgtac
      241 cagtgccagt agtggatgcc gttgtagtcg cccgtgagag ccgcgaagcg ccaccccgaa
      301 gcggcagcgg ccgtccgcca acgcgcgacc agcgtgtcgc cgtgccgcgg cgcgagcgcc
      361 agcggcgatg ccgcgcccgt ctgaccatat ctcccacgat agtagaacgt gttcagactc
      421 tcccacacaa gctcctgccg ggccgatatg ctcgtgtgca gatcgaactc cacgcctttc
      481 tccaggatgc gctggcccac gacgcgcgtt tccaggtcca gtacggtgtc agcgaagatc
      541 gatcgatgtt gcagctggtg attgcgaatc tgcaacacct tccagaacgg catcgggaag
      601 cgtggatgcg tgaggacggc catctgcaac ggaaagctga agacgtgagg gaaaaggatt
      661 ggcatgcccg cgtccgcgcg cagcccgctg agctggagga actcggcgag gtggcgccga
      721 tcgacccgat ggctcctcca tctcgcgcga atggctggac cgccgcccgt gccgtgcacg
      781 cccggggacg gatagaggcc ccgcagcatg tacgcgagga cggaggggcg ccggcggaat
      841 tcgatgatga cgtcagcggt catgattcct cggctcagcc cggcgctcct gagcctccag
      901 cttccgcagc cgtcgccagc ccgactccca gaggcgccgg aagggccggt cgaggaacca
      961 tcgctcgagc aatggtgatg ccctgaagag ccagcgcacc gcgacggcct catggccttc
     1021 gaccatgacg attccgttgt cctgagtaaa acggatctca ccctctccgc gccagaaccc
     1081 gcgataggtc catcgcacga gcgtgttttc ctggcgtgcg gtgatctttg cgacgagcgg
     1141 aaacgggaac cgcttcagcg gaacggcata ccagccgtcg ccgagctctc tgacggtccc
     1201 gccgaacccg taaagacgga cccaatcggc gggggtcgcg aaatagccga agaccacgcc
     1261 caccggcaac gcgaattgct ctttgaaccg aatgatcatc tgtcaccccc tggacgacgg
     1321 tccggcctga gtctggtcat cgtacatccg tcgtcacctg tcgcttcgag tccgccgagt
     1381 gcggcgccga agcgagatac gctacgccga agagcgccag caggaccgtc tgcaggatca
     1441 aagtgggcac cacgcgccag agcgctaccc aggcggctag cacgccaacg atctcagtga
     1501 ccgccaggcc gccgatgacc accgccgtca gtgccgcgat gaaacgccgc tccagcgggc
     1561 gctgccacgc ccacaccagc agcccagtcc agccaagcat caacgcggct gcagaaccca
     1621 tcgccaacaa gtagggtccg cccggatcct gaaatcccca gagggccgtg gccacagagg
     1681 gactgagcat cgggatgacg gccaaggcgt ccgtcaccgc gccggcgatg aacgcagcgc
     1741 gcagcagtcg aaatcctcta tctgcatcca tggcccctct cacggcagct cggtcacgac
     1801 gccctgcgcg agcgcgagcg cgacccgcgc ggactggaag gtggcgcgtg catccacgct
     1861 ctgcgtacgc gccgcattga ggcccagcaa agcggtgatc acatcggcgt tgccggccac
     1921 tccctgcgtg aagcgccgcc gcgcgagttc gagttcctgc tccgcgagcg ccagccgctc
     1981 gtccgtggcc ctgatctgct cagccgccga cgtcagttcg agcaacgcca cccgtacctc
     2041 gagcatgacc tgctgcacga ggtcgcggcg acgcacgtcc agctcgcgca tggcggaccg
     2101 ctgctcggcg atccgcccct cgcggcgcag gccgtcgaac accggtatcg agacctgcac
     2161 gccccacgaa taggtattca gcaggcgcgc ggtgctgttt cccgtgctcc cccaatcggc
     2221 gaagatggca agagcaggaa gacgttccgc cttgatcgcc gcaatctggc gctccacgcc
     2281 gtccatctgc accccgatca tgcgcaggtc cgcccgcgtc cgtagggctc gttcgttcgt
     2341 ctcagcctcg agcggggcgg gcagcgccgt gggcatctcc aggagcgagt cggcgagcgt
     2401 gaggggcgcg tcgaacggga gaccgagcgc gcggtgcaac tgtaacctgg cgcgctcgtg
     2461 ctcggtccgc gccgcgatca gttgtgccct ggtagccgcg agctgcgcct gggcccgcgt
     2521 cacgtcgagg gcgatgccca tgcccgcatc ccgctgattc cgcgcgatgc cgagcagctc
     2581 ctcagcgagt gtggagtccg cctgccgcgc acggagctgc gcgtccgccg ataacgcgcg
     2641 tacgtacaac atcgcagccg aggcagcggc ctgctgcgat tcgtttgtgg cgtcggcatc
     2701 ggcggcggcg accgtcgcac gcgcggcgcg tacccgggcg aatgacgaaa agtcgacgac
     2761 gctctgtcgc gccgtcgccc gcaggtccca ggtgcggacc ggacccaaca cctggccggc
     2821 ggggtcgaac aggtcgctcc cggtggtcgg atcacggagt gcgaggccga agcccgcgct
     2881 gttcaaggtg cgctcgccct ggctgagcac cccggcgacc gtcggcagca ggtcggcgtg
     2941 ctgctgccgc agccgcgctt cggccatgtc cacgcggtac cgcgcggcct ccgggcccgc
     3001 gctgcgctcc gcggcgaagc gggcggcgcc gcccagggtg agcgtgaccg gctgctgagc
     3061 ggctgcgggt cccgcgtgca gagcgaggag gcacgcgacg ccggcgatcc acgggaaggt
     3121 agcgaggctc ttcataggcg ctcctgggcc agggtggcag gctttccgct agacgcggac
     3181 gccgggcgaa gagcgaacgg cgccctgtcc cggggagtcg gctcgcccca tccagcggac
     3241 tgtgcgcgcg aaccgatagc cgaagtaggc ggcgaacatg ctcgcgacga acgtgaggaa
     3301 cccgatggcc tcgtacgggg cgctgccgag ctccggcgac atgaagaacc gctgcagaga
     3361 cagtggtagc gcgccgtcgt tcacagcgct gacggccacc ttgacggtgt gattccacaa
     3421 accaccgacc aggccgaacc cgccgatgac ccacaggttc agcagtccgt agagcacgag
     3481 aaccaacctg ttaccggtgc ggcggtagtg gctgctcagg gcgaccagaa tcgcgatgac
     3541 gatcgccccc accacgaacg cgggagtccc aaactcgtag gcgtgtgtga tcgaggcgag
     3601 ggttatggtc atcgatatgc tccacagcga ccgcgacgcg gcactcgtgg agcgactgga
     3661 ttgcggcgat gcagtcgtca tgtgcgtgtc ctcacgttgg agcgtggcgt gtgtccccag
     3721 acccgctg
//
LOCUS       DBNGZW010000646         3731 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1222370, whole genome shotgun sequence.
ACCESSION   DBNGZW010000646 DBNGZW010000000
VERSION     DBNGZW010000646.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3731
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1222370"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..853)
                     /locus_tag="AC16JC_15145"
     CDS             complement(<1..853)
                     /locus_tag="AC16JC_15145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5166084.1"
                     /translation="MRHLVIVPARNEGLFIGDMLMSLVRQTDPPDRIVIVDDGSSDET
                     APIVEAVAARHPTVQLLRHQPERSDVMGPSVVRAFTFAYCRLQASDYTYVSKFDADLV
                     FPETYCATVLSHLDAHPDVGLAGGVLHAEAHGEPVHVRAAPNHVAGAVKTFRTTALAA
                     IGGLEEVSGWDIVDQVKLRTLGWRTAALPQARVLHRREHGARNGLLQGKVDWGRGAWA
                     IGSHPLFVVARGLYRMLEPPYVIGGFAFWGGYLTAALRKVPRLRDAVVVSRLRQEQLQ
                     RLIPWGRP"
     gene            complement(856..2745)
                     /gene="asnB"
                     /locus_tag="AC16JC_15150"
     CDS             complement(856..2745)
                     /gene="asnB"
                     /locus_tag="AC16JC_15150"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002982358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HIR5166085.1"
                     /translation="MCGIVGIASREPVQRPDVLVRMRDTMAHRGPDDAGVWWSPDHRI
                     GLGHRRLAVIDLTPAGRQPMVDPETEACLTFNGEIYNYRELRHELDPTGRRFRSASDT
                     EVILHAYGRWGEDCLSRLRGMFAFALWDAPRRRLLLARDRCGEKPLFYWLDGRGTLQF
                     ASELKALMANPALPRVLDFAALEHYLAYAFVPGSRCILQGLHKLPAAHALSWSLETGA
                     VSRWRYWDPATASPAGPDPRSTDELTDEVHSLLSEAVRGQLVADVPIGVLLSGGLDSS
                     LITAVAAAQSNGGRRLRTFTVSFRGHGRFDESRHARLVAGHFGTEHHELEAAEIGPEL
                     LPCLARQFDEPMGDSSMLPTFLLATLVREHATVALGGDGGDELFGGYRYYSWIPRVER
                     LRALAPAALRRAVAAAGRALPLGVPGRNQLVGCDGDATRAIAHIDLYFDAATRAALLR
                     PLRLAGWRLDSAPETSKAAACPPRLSPVRRAMVNDFRRYLPDDVLVKVDRASMLASLE
                     VRAPFLDHRLVAFALGSVPDRALAWASHRKILLRRLAQRVLPAAFDVERKQGFSVPLA
                     RWLEGSWGRYVREVLADADRRLFDRDMVRKLGAGRRGVGNAKRLFALTLFELWRREYD
                     VRVEA"
     gene            complement(2748..>3731)
                     /locus_tag="AC16JC_15155"
     CDS             complement(2748..>3731)
                     /locus_tag="AC16JC_15155"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5166086.1"
                     /translation="NIAPALVHAHFGPDAALIMPLARALGVPLVITFHGYDATMADAQ
                     ARRSFFLHRRYLGRRAALQREGRLFLAVSGFVRDRLLARGYPPERTVVHYVGVPLDRF
                     RPVPGRVREPLVLFVGRLSPEKGCAHLVRAMRRVEEDCPDAELVVLGDGPLRNALERL
                     ARASVPRCRFLGSGSQEEVIAWMARAQVLCVPSVTLPSEEAEGFGLVFAEAQAMALPV
                     VSSVVGGIPEAVAHGETGFLAPEGDEAMLSAHLVRLLRDPALRVRMGEAGRRRAERLF
                     DHRGQTRALERLYDIALNGFERGRALRPAHGLGNRSPFGGAPGAHVRASAG"
BASE COUNT          593 a         1374 c         1312 g          452 t
ORIGIN      
        1 ggggccggcc ccagggaatc aggcgctgca gctgttcctg ccggagtcgc gataccacga
       61 cggcatcgcg cagccgcggg accttccgga gggcggccgt caggtaccct ccccagaacg
      121 cgaacccgcc gatgacgtac gggggctcga gcatccgata caggccgcgc gcgacgacga
      181 acaacgggtg ggagccgatg gcccacgcac cgcgccccca atcgactttc ccctggagga
      241 gcccgttgcg ggcaccatgc tcgcgccggt gcagcacgcg cgcctgtgga agcgccgcgg
      301 tccgccagcc cagggtgcgc agcttgacct gatcgacgat gtcccagccc gaaacctcct
      361 ccagcccgcc gatcgcggcg agcgcggtcg tgcggaacgt cttcaccgcc cccgcgacgt
      421 ggttgggcgc cgcgcggacg tggaccggct cgccgtgagc ctccgcgtgc aggaccccgc
      481 cggccagccc cacgtcggga tgcgcgtcca gatggctcag gacggtggcg cagtaggtct
      541 ccgggaacac caggtccgcg tcgaacttcg acacgtaggt gtagtccgag gcctggagcc
      601 ggcagtaggc gaaggtgaag gcacgcacga ccgacggccc catgacgtcg cttcgttcgg
      661 gctggtgccg gagcagctgc acggtcggat ggcgcgccgc caccgcctcg acgatcgggg
      721 ccgtttcgtc cgaggatccg tcgtcgacga tcacgatgcg gtccggcggg tccgtctgcc
      781 gcaccagcga catgagcatg tcgccgatga acaggccctc gttccgggcc gggacgatga
      841 cgagatggcg cacggctagg cctccacccg cacgtcgtat tcccggcgcc acagctcgaa
      901 cagcgtcagc gcgaacagcc gcttcgcgtt gcccactccc cggcgtccgg cgccgagctt
      961 ccgcaccatg tcccggtcga acagccgccg gtcggcatcc gcgaggacct cccgcacata
     1021 gcgcccccag gacccctcga gccagcgagc cagcggcacc gagaacccct gtttgcgctc
     1081 gacgtcgaac gccgcaggca gcacgcgctg cgccaggcgc cgcagcagga tcttccggtg
     1141 cgacgcccag gcgagcgccc gatcggggac cgacccgagg gcgaaggcca cgaggcggtg
     1201 atccaggaac ggggcgcgga cctcgagcga cgcgagcatg ctggcccgat ccaccttgac
     1261 cagcacgtcg tcggggaggt agcggcggaa gtcgttgacc atcgcgcggc gcaccggcga
     1321 gagccggggc gggcaggcgg ccgccttgga cgtttcggga gccgaatcga gccgccaccc
     1381 ggcgagccgg agcggccgca gcagcgcggc ccgcgtcgcc gcgtcgaagt agagatcgat
     1441 gtgggcgatg gcgcgggtcg cgtcgccgtc gcacccgacg agctgattcc gtcccggcac
     1501 gcccagcggc agcgcgcgac cggccgcggc cacggcccgg cgcagcgccg ccggggcgag
     1561 cgcgcgcagc cgctcgaccc gcggaatcca ggagtagtag cgataaccgc cgaacagctc
     1621 gtcgccgcca tcgccaccga gcgcgaccgt cgcgtgctcc cgcacgaggg tggcgagcag
     1681 gaacgtcggc agcatcgacg agtcgcccat cggctcgtcg aattgccggg cgaggcacgg
     1741 cagcagctcg gggccgattt ccgccgcctc gagctcatgg tgctccgtgc cgaaatgccc
     1801 ggccacgagc ctcgcgtggc gagactcgtc gaaccgcccg tgtccccgaa acgagacggt
     1861 gaaggtgcgc agtctcctcc cgccgttgct ctgggcggcg gcgacggcgg tgatcaggct
     1921 ggagtcgagc ccgccgctga gcagcacgcc gatgggaacg tcggcgacga gctgtccccg
     1981 caccgcctcc gacagcaggg agtgcacctc atcggtcagc tcgtcggtcg atctcgggtc
     2041 cggccccgcg ggagacgccg tcgccgggtc ccagtagcgc cagcgggaaa ccgcgccggt
     2101 ctcgagcgac cagctcaggg catgggcggc ggggagcttg tgcagtccct ggaggatgca
     2161 gcgcgacccc ggtacgaacg cgtaggcgag gtaatgctcc agggcggcga agtcgagcac
     2221 gcgcggcagc gccgggttcg ccatcagggc cttcaactcc gaggcgaact ggagcgtccc
     2281 ccgaccgtcg agccagtaga acagcggctt ttccccacag cggtctcgcg ccagcagcag
     2341 ccgccgccgc ggcgcgtccc acagggcgaa cgcgaacatg ccgcgcaggc gcgagagaca
     2401 gtcttcgccc cagcgcccat acgcgtgcag gatcacctcc gtgtcgctcg cgctccggaa
     2461 acgccttccg gtcgggtcga gctcgtgacg cagctcgcga tagttgtaga tctcgccgtt
     2521 gaaggtgagg cacgcctccg tctcgggatc gaccatgggc tggcggcccg cgggcgtcag
     2581 atcgatgacc gccagccggc ggtgtcccag gccgatgcgg tggtccggcg accaccacac
     2641 gccggcgtca tccggtcccc ggtgcgccat cgtgtcccgc atgcgcacga ggacatcggg
     2701 ccgctggacc ggctcacgcg acgcgatccc cacgatgccg cacatcgcta cccggcgctc
     2761 gcccggacat gcgccccggg cgctccgccg aacggagagc ggttcccaag tccgtgcgcc
     2821 ggccgcagtg cccgcccgcg ctcgaatcca ttcaacgcga tgtcgtagag ccgctcgagc
     2881 gcacgcgtct ggccacggtg atcgaacagc cgctcggcgc ggcggcgtcc cgcttcaccc
     2941 atccggacgc gcagcgcggg atcgcggagc agccgcacga ggtgagcgga cagcatcgcc
     3001 tcgtctccct cgggcgcgag gaaccccgtc tccccgtggg cgaccgcctc ggggatgccg
     3061 ccgacgacac tcgacaccac cggcagcgcc atcgcctgcg cctcggcgaa gacgagcccg
     3121 aacccttccg cctcctccga cggcagcgtg acgctcggca cgcacaggac ctgtgcgcgc
     3181 gccatccacg cgatgacttc ctcctgcgac ccggatccca gaaagcgaca cctggggacg
     3241 ctcgcccgcg cgaggcgctc cagggcgttc cgcaggggtc cgtcgcccag cacgaccagc
     3301 tcggcgtcgg gacagtcctc ctccacccgg cgcatggcgc gaaccagatg ggcacacccc
     3361 ttttcgggcg acaggcggcc gacgaacagc acgagcggct cgcggactcg accggggacg
     3421 ggccggaagc ggtccagggg cacgccgacg taatgcacga cggtgcgctc cggcgggtac
     3481 ccccgggcca ggagtcggtc tcgcacgaac ccggagacgg cgaggaacaa gcggccttcc
     3541 cgctgcaacg cggcgcggcg tccgagatac cgccgatgga gaaagaacga ccggcgcgcc
     3601 tgggcatcgg ccatcgtggc gtcgtagccg tggaacgtga tgaccagcgg tacgccgagg
     3661 gcgcgcgcca gcggcatgat cagcgcggcg tccgggccga agtgggcgtg gaccagtgcc
     3721 ggggcgatgt t
//
LOCUS       DBNGZW010000647         4100 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_402653, whole genome shotgun sequence.
ACCESSION   DBNGZW010000647 DBNGZW010000000
VERSION     DBNGZW010000647.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4100
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_402653"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="AC16JC_15160"
     CDS             <1..189
                     /locus_tag="AC16JC_15160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166087.1"
                     /translation="AMAEQMEQAKAAQAAPEGQPEAAKPDSGPSAKDAAKATAEQAAK
                     DAAAQKVKKGLGGLFRRP"
     gene            451..1866
                     /locus_tag="AC16JC_15165"
     CDS             451..1866
                     /locus_tag="AC16JC_15165"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HIR5166088.1"
                     /translation="MHAMKTGRIARAVATSLAVTLMACSADLATAVDTFSNPGICPAT
                     PAALPAADVTVTLDAAQRFQTIQGFGSTERLFDDPHVTNTFDASTGRAAATAIPPASD
                     QAKILDALYVDLGLTRIRIHPEGFEPVNDNADPNAADLSKFNFGNRGADGHIAAVKTL
                     LPRGVTTYFVSPVVLESWMNESNPDEYVEWVMVMLRHWRQQGLEMPYYSLLNEPGYYV
                     GLKSWSGEWMRDVAKKLGAKLAAENFRTKLVIPDDVSPSEAYARIQTILADPEARKYV
                     GALAYHLYGRGGEDKVAQIGKQYGIPVWMTEFSTPGDWRAWAVVMQQMLGDDDVSAID
                     YMWGYFGDWDRSQLIRLKLSGTTYVGYDMTKQYYVMGQYSRYVRPGAVRIAATSSDPD
                     VKVVAFVDGAKPIVVVTNLGARDHTVRFEFGSAGVCGGRLQAVRTSEGESWSALPEVT
                     LDQPRFAAPAPAGSVTTFAGR"
     gene            1964..2362
                     /locus_tag="AC16JC_15170"
     CDS             1964..2362
                     /locus_tag="AC16JC_15170"
                     /inference="COORDINATES: protein motif:HMM:NF017451.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5166089.1"
                     /translation="MRDLRKLEVALRARELVIAIYEATATFPPTERYGLAAQMRRAAV
                     SIASNIAEGAGRGGEREFLHFLYMALGSAFELAIQVDLAIALCLVDNAEASPLADRTD
                     HVQRMLNRLTGSMRRKLARSQQGSGAERQI"
     gene            complement(2459..4024)
                     /locus_tag="AC16JC_15175"
     CDS             complement(2459..4024)
                     /locus_tag="AC16JC_15175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5166090.1"
                     /translation="MSAPTSPTNPPVAPERAPATYESPGGRRYVPGLRWWICGLLFFA
                     TTINYVDRQSLSVLKTMLERQMNWSEADYGWIQFAFTAAYAAFPSIAGRVMDSMGVKF
                     GLALALVAWSTMSMLTALARGTIGFAVARFFLGAAEAGNFPASIKAIGQWFPQRERAL
                     ATGLFNSGTNVGVMVSFITVMFAERFGWQFAFIAIGAIGFLWLIFWQIGFHAPENHPN
                     LSPEELAHIKSDQAQNQEKLKLHWTTLLRFRQIWPFLLAKLLTDPVWWFYLYWLPSYL
                     AKERGQNILGSAYLLTAIYTAASLGSIAGGWLSGFLIKRGWRIATARYAAMGIAAVCA
                     PFAILAYYTSSFTLCLVLLGVVTAAHQAWSANLFTSATDLFPSKVTGSVVGLGATTGG
                     LGGMFLTLLAAMSIQWIGNQSAVFVWAGLMHPLSLLIFWLVLGRDFKMADVDAVLDTS
                     TVSRPLAAAGGALIVVGLALVALIWANWDASVRAASLAGAAQAATAAVGVVLVGLTLL
                     YAGLRRRAMVVAV"
BASE COUNT          728 a         1437 c         1353 g          582 t
ORIGIN      
        1 gcgatggccg agcagatgga gcaggccaag gccgcgcagg ccgcgccgga aggccagccg
       61 gaagccgcca agccggacag cggcccgtcg gccaaggatg cggcgaaggc gacggccgag
      121 caggccgcca aggatgcggc ggcgcagaag gtgaagaagg gactcggcgg actcttccgc
      181 cgtccgtagt cgccgtcgat cacctcgtgg gagcggctcg cggccgctct cacgaacatc
      241 ctgtagcgca ggtcacacgc ggagcggcaa ccccgacctc ggaatacttt tcgcgtagga
      301 ccgacgcgtt cgtggcgccg tggtggttcc tcgggagccg ctcccacggt ccccgtagtg
      361 gacgcgcggc tcgacgccgc gtgccgccgg ccgagagagt gccttcacgt cgacctctct
      421 cggctccctc gaagagactt catgtcagtc atgcacgcaa tgaaaaccgg acgcatcgcg
      481 cgcgcggtcg cgacgagcct cgccgtcacg ctcatggcgt gcagcgccga cctcgcgacc
      541 gccgtcgaca cgttctccaa tccggggatc tgccccgcca cccccgccgc cctgccggcg
      601 gccgacgtca ccgtcacgct ggacgctgcg cagcggttcc agacgatcca ggggttcggc
      661 tcgacggagc gcctgttcga cgacccgcac gtcaccaaca ccttcgacgc gagcaccggc
      721 cgcgccgccg ccaccgccat cccgccggcg tccgatcagg cgaagatcct cgacgccctg
      781 tacgtcgacc tcggcctcac gcgcatccgc atccatcccg aggggttcga gccggtcaac
      841 gacaacgccg atccgaacgc ggccgacctg tcgaagttca acttcgggaa ccgcggggcg
      901 gacggtcaca tcgcggccgt gaagacgctg ctgccgcgcg gcgtcaccac ctacttcgtg
      961 tccccggtgg tgctcgagtc ctggatgaac gagagcaacc cggacgagta cgtcgagtgg
     1021 gtgatggtca tgctgcgcca ctggcgccag caggggctcg agatgccgta ctattcgctc
     1081 ctcaacgagc cgggctacta cgtgggcttg aagtcgtggt cgggcgagtg gatgcgcgac
     1141 gtcgcgaaga agctcggagc gaagctcgcc gcggagaact tccgcaccaa gctcgtgatc
     1201 cccgacgacg tgtcaccgtc cgaggcgtac gcgcggatcc agacgatcct cgccgacccg
     1261 gaggcgcgaa agtacgtcgg cgcgctcgca taccatctct atggccgcgg tggcgaggac
     1321 aaggtcgcgc agatcgggaa gcagtacggg atcccggtct ggatgacgga gttctcgacc
     1381 ccgggcgact ggcgcgcctg ggccgtggtg atgcagcaga tgctcggcga cgacgacgtc
     1441 tcggcgatcg actacatgtg gggctacttc ggcgactggg accgctcgca gctgatccgc
     1501 ctgaagctga gcgggacgac ctacgtcggc tacgacatga ccaagcagta ctacgtgatg
     1561 ggccagtact cgcgctacgt gcggcccggc gcggtgcgca tcgccgcgac gtcgtccgat
     1621 cccgacgtca aggtcgttgc cttcgtggac ggcgcgaagc cgatcgtcgt cgtgacgaac
     1681 ctcggcgcgc gcgaccacac ggtccgtttc gagttcggct ccgccggcgt gtgcggcggg
     1741 aggctccagg cggtgcgcac gagcgaaggc gagagctggt ctgccctccc cgaggtcacg
     1801 ctggaccaac cccgcttcgc cgcccccgca ccggccggca gcgtgacgac gttcgcggga
     1861 cggtaatcgc gagttcgcgg gttcgcgggt tcgcgggttc gcgattgcgg tgtggcgcgc
     1921 gtgattggtg cgtacgtttc gcagcgctta cgtaagctcg agcatgcgcg atttgcggaa
     1981 gctcgaagtt gcactacggg cgcgtgagct cgtcatcgcc atatacgagg cgaccgccac
     2041 attcccgccg accgagcgtt acgggctcgc cgcgcagatg cgaagggcag cggtgtccat
     2101 cgcgagcaac atcgcggagg gggccggcag aggaggagaa cgcgagtttc ttcacttcct
     2161 ctacatggcg ctcggctccg cattcgaact agcgattcag gtggatctcg ccatcgccct
     2221 ctgcctcgtc gacaacgccg aggcttcgcc tctcgccgat cgcacggacc acgttcaacg
     2281 catgctgaac cgattgacgg gaagcatgcg acggaagctt gcgaggagcc agcaagggag
     2341 cggagcggag cgacagatct gagcggccgg caggtgcgaa cctccgaacc tccgaacctc
     2401 cgaacctccg aacccccgaa cccccgaacc cccgaacccc cgaacccccg aacccccgct
     2461 acaccgcaac caccatcgcc cgccttctga gccccgcgta cagcagcgtc agcccgacca
     2521 gcaccacgcc gacggcggcg gtcgcggcct gggcggcgcc ggccagactg gccgcgcgta
     2581 cgctcgcatc ccagttcgcc cagatcagcg ccacgagcgc gagcccgacg acgatcagcg
     2641 cgccccccgc cgcggccagt ggccgcgaga ccgtgctggt gtcgagcacc gcgtccacgt
     2701 cggccatctt gaagtcccgg ccgagcacga gccagaagat caggaggctg agcgggtgca
     2761 tcagccccgc ccacacgaac accgcgctct ggttcccgat ccactggatc gacatcgcgg
     2821 ccagcagcgt gaggaacatc ccgcccaggc cgcccgtcgt cgcccccagc ccgaccaccg
     2881 aacccgtcac cttcgacggg aacaggtccg tcgccgacgt gaacaggttg gccgaccagg
     2941 cctggtgcgc ggcggtgacg acccccagga gcacgaggca gagggtgaag ctgctcgtgt
     3001 agtacgcgag gatcgcgaac ggcgcgcaca ccgccgcgat ccccatcgcc gcgtagcgcg
     3061 ccgtggcgat ccgccacccg cgcttgatca ggaagcccga gagccagccc cccgcgatcg
     3121 aaccgaggct cgccgcggtg tagatcgccg tcagcaggta cgcgctgccg aggatgttct
     3181 gtccccgttc cttcgccagg tacgacggca gccagtagag gtagaaccac cacaccggat
     3241 cggtcagcag tttggcgagc aagaagggcc agatctgcct gaaacgcagc agcgtcgtcc
     3301 agtgcagctt cagcttctcc tggttctgcg cctggtccga cttgatgtgc gccagctcct
     3361 ccggcgacag gttcggatgg ttctccggcg cgtggaagcc gatctgccag aagatgagcc
     3421 acaggaaccc gatggcgccg atggcgataa aggcgaactg ccacccgaac cgctcggcga
     3481 acatcacggt gatgaacgac accatcacgc cgacgttcgt ccccgagttg aacagccccg
     3541 tcgccagggc gcgctcccgc tgcgggaacc actgcccgat cgccttgatc gacgctggga
     3601 agttccccgc ttcggccgcg ccgaggaaga accgcgcgac ggcgaagccg atcgtgccgc
     3661 gcgccagggc ggtcagcatc gacatcgtcg accacgccac cagcgcgagc gcgagcccga
     3721 acttcacgcc catggaatcc atcacgcggc cggcgatgga cgggaacgcc gcgtacgccg
     3781 ccgtgaacgc gaactggatc cacccgtagt cggcctcgct ccagttcatc tgccgctcga
     3841 gcatcgtctt gaggacgctc agcgactgcc ggtccacgta gttgatcgtc gtggcgaaga
     3901 acagcaggcc gcagatccac cagcgcagac cggggacgta ccggcggccg cccggtgact
     3961 cgtacgttgc cggcgcccgc tcgggtgcca cggggggatt ggtgggtgac gtcggagcgg
     4021 acattggtgg gttcagcggg taaagtctgt gcggaacgga agaggccgcc ccgaacacgg
     4081 tcaggagtga gaataagagc
//
LOCUS       DBNGZW010000648         7149 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_672168, whole genome shotgun sequence.
ACCESSION   DBNGZW010000648 DBNGZW010000000
VERSION     DBNGZW010000648.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7149)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7149)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7149
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_672168"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1212)
                     /locus_tag="AC16JC_15180"
     CDS             complement(<1..1212)
                     /locus_tag="AC16JC_15180"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF014895.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HIR5166091.1"
                     /translation="MSERLLRGVGVSPGIAIGPALVVASSLPDVPHRVVPRSQVGKEV
                     RRLRAAVRDVQRHVSELKERAAQRAGPDEARIFDAQLLMLEDEDFIGAIEDLIRENHL
                     TAEKAFEFKALEQRDAWMSVGSARLKDRIADMNGVMIRVVRHLMHLGDDDLEGVTQPS
                     IVVAREVFPGLTVQLDRSQVIAIASEEGTRTSHAAILAHSMGMPAVMGIMGALGRITS
                     GTMLLLDGTRGTVLLDPTPAELAEAKALGLRDQARGIELERGVSLPAVTTDGVRVALR
                     GNVDLPEEIDAARAHGAEGVGLLRTEFFITGHSTLPTEEEQTRYFSRVGKAFPGHPVV
                     IRSYDLGGDKFPAAFQAPYEANPFLGWRAIRVCLDHPDLFRGQIRAVLHAATDADIRL
                     MIPLVTRLAEVQ"
     gene            complement(1209..1553)
                     /locus_tag="AC16JC_15185"
     CDS             complement(1209..1553)
                     /locus_tag="AC16JC_15185"
                     /inference="COORDINATES: protein motif:HMM:NF012599.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HIR5166092.1"
                     /translation="MAVALIEREARIVNPLGLHARPAAEFVKTASRFKSAIHVSKDGL
                     SVNGKSIMGVMMLAAERGSSMNIRAEGDDAEVAMEALLALVAAGFNEMHLTGSEADLE
                     DVDDEPGTEVRS"
     gene            complement(1538..2296)
                     /locus_tag="AC16JC_15190"
     CDS             complement(1538..2296)
                     /locus_tag="AC16JC_15190"
                     /inference="COORDINATES: protein motif:HMM:NF015570.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HIR5166093.1"
                     /translation="MTPLPPAPPLRSSFLRLFMVQASWNYDRLLGVGMGLAEEPLLRA
                     LKGEAADGAAYRAAVARGARFFNAHPYLCGLAVGATARAEYDGAPPEEVERLRTALCG
                     PLGALGDRLVWAGWLPLLATAAVAAVALGARWWAAIGFLLIYNAGHVALRWWALRAGW
                     AAGTGVASALRHPVLQRPLAFIVPAVAFVAGLCLPLAAGWLVRPFPLVLAVAGAAVAA
                     AGVTLLRWLPGRVKSLWIGLAAVALGMAGGWLWR"
     gene            complement(2293..2970)
                     /locus_tag="AC16JC_15195"
     CDS             complement(2293..2970)
                     /locus_tag="AC16JC_15195"
                     /inference="COORDINATES: protein motif:HMM:NF015566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HIR5166094.1"
                     /translation="MSEVTLALLLVWGTLVGLDLVSVPQMMIARPLVAGTFAGLLVGD
                     LETGLRLGIVFELFQFDILPVGAARYPEYGPATVAAVAAAHWLGRSDAWGLGVGIGVL
                     IALLAGNTLHWLRTANARAVRASAAQLETGDVHVLARIHAAGLARDVIRAGLVTATGL
                     AAGWLLWRAGATRLPPHLFALSSALGGGAALAAGVAGLLRIVGRGAGLRWFAAGLAAG
                     VMALWIA"
     gene            complement(2967..3443)
                     /locus_tag="AC16JC_15200"
     CDS             complement(2967..3443)
                     /locus_tag="AC16JC_15200"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-carbohydrate
                     phosphotransferase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0034219 - carbohydrate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HIR5166095.1"
                     /translation="MSLALVRLDDRLIHGQVVVGWGQPLNAAFIVLVDDEVRGSEWEQ
                     DLYRMGVPANMEVVFASINEAERRLPEWEADDRPGILLTGDIDTMAAVVAAAAGRIRR
                     VNVGGVHHRAGRTERLRYVYLTEEEAGKLRRLASNGVEVSAQDVPTARAVPIGEFL"
     gene            complement(3440..3817)
                     /locus_tag="AC16JC_15205"
     CDS             complement(3440..3817)
                     /locus_tag="AC16JC_15205"
                     /inference="COORDINATES: protein motif:HMM:NF015567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HIR5166096.1"
                     /translation="MSELRGVVVSHAELAAALVAAVRAISGVDDALVAVSNEGCDPRA
                     LAARIEAAIGGVPAVLFVDLPGGSCLTGAVRMAHDRSDVSVVTGVNLAMLLDFVFHRD
                     TSPAEAARRAVDAGGRAIRTPAA"
     gene            complement(3810..4778)
                     /gene="hprK"
                     /locus_tag="AC16JC_15210"
     CDS             complement(3810..4778)
                     /gene="hprK"
                     /locus_tag="AC16JC_15210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006109 - regulation of carbohydrate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HIR5166097.1"
                     /translation="MNQRPRVRDLLARKGALELESLTGEQGLDREIPSSEASSPGLVL
                     AGYTARFVGDRIHILGETEISYLGSLEPAERRRSLELFFKFDLPCVVITKSQEAPAEL
                     LELAGTRQVPVIRTSLKTAEFYRRLKPYLDDVFAPATTLHGSLADVYGVGLLFMGRSG
                     IGKSECVLDLVERGHRLVADDLVHVTRRGVDILIGRGHEMARHHMEIRGIGLIDIKQL
                     FGIRAVRQQKRIEVVVQLEDWDAAREYDRTGLETQQTTILGVAVPLVTVPLNPGKNLT
                     VICEIVAMSHLLRYSGVDAARNFNDRLIRQMSERASLREYLEEDYE"
     gene            complement(4775..5101)
                     /locus_tag="AC16JC_15215"
     CDS             complement(4775..5101)
                     /locus_tag="AC16JC_15215"
                     /inference="COORDINATES: protein motif:HMM:NF014534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043024 - ribosomal small subunit binding
                     [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HIR5166098.1"
                     /translation="MDITVTARHCDVTADLRLRARALVERLARVAARPHDGQVIFSEN
                     HGVAAAELRLHAARGALFVGRAEGIDHRTALDRAAARVRRQLDKSPAKRSGPARRRSV
                     ARNEAE"
     gene            complement(5106..6224)
                     /locus_tag="AC16JC_15220"
     CDS             complement(5106..6224)
                     /locus_tag="AC16JC_15220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5166099.1"
                     /translation="MRILLVNWNDRENPHAGGAEIHLHEIFGRLARQGHRVDLVASGW
                     AGAAAAAEVDGLRVRRVGGRLSFALLARGAVRAALRQPHGYDVVVEDVNKLPLFLPSL
                     TDLPFCAIVPHLFGTTAFVEASWPVAAVVWAAERAIPRVYARAAFHVISNSTRDDLVA
                     RGVAAERIEVIYPGIDATWYHPDSATPRAARPTFLYVGRLKRYKGVDIALRALALAHA
                     GGVDATLEIAGAGDDRPRLEAEVRRLALMDAVRFLGFVSEEEKRRLLRSAWAVVFPSP
                     KEGWGISNVEAAACGTPALASDSPGLRETVRHGETGFLVPHRDAAELARRIGELARDP
                     ALVARLGVAARAFALPLTWDRAAHLTGAHLERVIAHWR"
     gene            complement(6221..6634)
                     /gene="nusB"
                     /locus_tag="AC16JC_15225"
     CDS             complement(6221..6634)
                     /gene="nusB"
                     /locus_tag="AC16JC_15225"
                     /inference="COORDINATES: protein motif:HMM:NF013216.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HIR5166100.1"
                     /translation="MERPETKARARALQLLYAWDLSGHPSIDTVVTRLARTYGGIPVG
                     YDRGAELAARAVAGQAEYDAQLSRLSEHWRLERVGVVEKNILRLAMAELDEGSTPARV
                     VMDEAVKLAHWFGGGRAPAFVNGVLDAVARELGRL"
     gene            complement(6615..7055)
                     /gene="ribH"
                     /locus_tag="AC16JC_15230"
     CDS             complement(6615..7055)
                     /gene="ribH"
                     /locus_tag="AC16JC_15230"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HIR5166101.1"
                     /translation="MAIVVSRYNELITRRLLDGARDALREAGVPDSHVEEFWVPGAFE
                     LAVAAEEAAVSGRFRAIVALGCVIRGETPHFEYVAGEAARGLGNVALAHRVALGFGVL
                     TTENQAQAEARAGGAAGNKGYEAAAAALQTADVLHRVRRGTARD"
BASE COUNT         1087 a         2726 c         2414 g          922 t
ORIGIN      
        1 ctgcacctcg gcgagacgcg tcaccagcgg gatcatgaga cggatgtcgg cgtccgtggc
       61 ggcgtgcaac acggcgcgga tctgcccgcg aaacagatcg ggatggtcga gacacacccg
      121 gatcgcgcgc cagccgagaa acgggttcgc ctcgtacggc gcctgaaagg cggccggaaa
      181 cttgtcgccc ccgaggtcat aactccggat caccaccgga tgcccgggga acgccttgcc
      241 gacccgcgag aagtagcggg tctgctcctc ctcggtcggg agggtgctgt gccccgtgat
      301 gaagaattcg gtgcgcagca gaccgacgcc ttccgcgccg tgggcacgcg ccgcgtcgat
      361 ctcctcggga agatccacgt tgccgcgcag cgccacgcgc acgccgtcgg tcgtgacggc
      421 ggggaggctc acgccgcgct cgagctcgat cccgcgcgcc tggtcccgga ggccgagcgc
      481 cttcgcctcg gcgagctccg ccggcgtggg atcgagcagc accgtgccgc gcgtcccatc
      541 cagcagcagc atggtgccgg acgtgatgcg acccagcgct cccatgatcc ccatcacggc
      601 cggcatgccc atggaatggg ccaggatggc ggcatgcgag gtgcgggtgc cctcctcgct
      661 ggcgatcgcg atcacctgcg agcgatcgag ctggacggtg aggccgggaa acacctcccg
      721 cgccacgacg atggacggct gggtgacgcc ttccaggtcg tcgtcgccta gatgcatgag
      781 gtggcggacc acgcggatca tcacgccgtt catgtccgcg atgcgatcct tgagtcgcgc
      841 gctgccgacg ctcatccagg catcgcgctg ctccagggcc ttgaactcga acgccttctc
      901 ggctgtgaga tggttctcgc gaatcaggtc ttcgatcgcg ccgatgaaat cctcatcctc
      961 gagcatcagc agctgcgcgt cgaagatgcg ggcctcgtcg gggccggcgc gctgcgcggc
     1021 acgctccttg agctcgctca catggcgctg cacgtcgcgc acggcggccc gcaaccgccg
     1081 gacctccttc cccacctgcg accgcggcac cacccggtgc ggcacgtccg gcagcgagga
     1141 tgccacgacc agcgccggtc cgatcgcgat cccgggcgag actccgacgc cgcgcagcag
     1201 ccgttcgctc acgaacgcac ctccgtgccg ggctcgtcgt cgacgtcctc gaggtccgct
     1261 tccgagcccg tcaggtgcat ctcgttgaat cccgcggcga cgagggccag caacgcctcc
     1321 atcgcgacct cggcgtcgtc gccttcggcc cggatgttca tggaggagcc gcgctcggcg
     1381 gcgagcatca tcacccccat gatgctcttg ccgttgacgc tgagcccgtc cttgctcacg
     1441 tggatcgcgc tcttgaaccg gctcgccgtc ttcacgaact ccgccgcggg ccgcgcgtgc
     1501 agcccgagcg gattcacgat gcgggcctcc cgctcgatca gcgccacagc cacccccccg
     1561 ccatccccag cgccaccgcg gcgagaccga tccacagcga tttgacgcga cccggcagcc
     1621 agcgcagcag cgtcaccccg gccgccgcca cggcggcgcc ggccaccgcc agcacgagcg
     1681 ggaagggacg caccagccag cccgccgcga gcggcaggca gaggccggcg acgaacgcca
     1741 ccgccggcac gatgaacgcc agtggtcgct gcagcaccgg gtggcggagc gcgctggcca
     1801 cgcccgtgcc cgcggcccat ccggcgcgca gcgcccacca gcgcaacgcc acgtgccccg
     1861 cgttgtaaat caggaggaag ccgatggccg cccaccagcg cgcacccagg gcgacggcgg
     1921 cgaccgccgc ggtcgcgagc agcggcagcc atccggccca gacgagccgg tcgcccaggg
     1981 cacccagtgg accgcacaac gccgtgcgca gccgctccac ttcttcgggc ggtgccccat
     2041 cgtactccgc gcgcgcggtg gctccgacgg ccagaccgca cagataggga tgggcgttga
     2101 agaaccgcgc cccgcgcgcc acggccgcgc ggtaggcggc gccgtcggcg gcctcgccct
     2161 tcaacgcccg caacagcggc tcctccgcga ggcccatccc gacacccagc aggcggtcgt
     2221 agttccacga cgcctgcacc atgaacaggc ggaggaacga ggagcgcagc ggcggtgccg
     2281 gcggcagcgg cgtcacgcga tccacagcgc catcaccccg gcggccaatc cggcggcgaa
     2341 ccagcgaagt cccgcgcccc gccccacgat ccggagcaga ccggcgaccc cggccgccag
     2401 cgccgcacca ccgccgagcg ccgagctgag cgcgaagagg tgcgggggaa gacgcgtggc
     2461 gccggcgcgc cacagcagcc acccggcggc gaggccggtc gcggtcacga gaccggcgcg
     2521 gatcacgtcg cgcgccagac cggcggcatg gatccgcgcc agcacgtgca cgtccccggt
     2581 ctcgagctgc gccgccgacg cgcgcacggc gcgggcgttg gcggtacgca gccagtgcag
     2641 cgtgttcccc gcgagcaggg cgatcagcac tccgatcccc acaccgaggc cccacgcgtc
     2701 gctgcgaccg agccagtggg cggcggccac cgccgcgacg gtcgccgggc cgtattcggg
     2761 atagcgtgcc gcaccgaccg gcaggatgtc gaactggaac agctcgaaca cgatccccag
     2821 gcgcaacccg gtctcgagat cgccgacgag caggccggcg aacgtgccgg ccacgagcgg
     2881 gcgcgcgatc atcatctgcg ggacgctgac gaggtcgagt cccacgagtg tgccccacac
     2941 gaggagcagg gcgagggtca cctcgctcac aggaactccc cgatcgggac ggcgcgggcc
     3001 gtcggcacgt cctgcgccga gacctcgacg ccgttggagg cgagccggcg cagcttgccg
     3061 gcttcctcct cggtgaggta cacgtagcgc aaccgctcgg tgcgtccggc gcgatggtgg
     3121 acgccgccga cgttgacgcg gcgaatgcgc cccgccgcgg cggccacgac ggcggccatc
     3181 gtatcgatgt cgcccgtcag gagaataccg ggccggtcgt cggcctccca ttcggggagc
     3241 cgccgctccg cctcgttgat cgaggcgaac accacctcca tgttcgccgg gacgcccatc
     3301 cggtagaggt cctgctccca ctcgctgccg cgcacttcgt cgtctaccag cacgatgaag
     3361 gcggcgttga ggggttgtcc ccaccccacc accacctgcc cgtggatcag gcgatcgtcg
     3421 aggcgcacga gggcgaggct catgcggccg gggtgcggat cgcgcgcccg ccggcgtcca
     3481 cggcgcgccg cgccgcttcc gcgggcgagg tgtcgcggtg aaacacgaag tcgagcagca
     3541 tggcgaggtt cacgccggtc accaccgaga catcgctgcg gtcgtgcgcc atgcggacgg
     3601 cgccggtcag gcaggagccc cccggcaggt cgacgaacaa cacggcaggc acgccgccga
     3661 tggccgcctc gatgcgcgcg gccagggccc gcgggtcgca gccttcgttg ctgaccgcca
     3721 ccagcgcgtc atccaccccg ctgatcgcgc gcacggcggc gaccagcgcc gccgcgagct
     3781 cggcatgcga gacgacgacc ccccgcagct cactcatagt cctcttcgag atactcccgc
     3841 agcgaggcgc gctcgctcat ctggcggatc agccgatcgt tgaagttcct ggccgcgtcc
     3901 acgccgctgt agcgcagcag atggctcatg gccacaatct cgcagatcac cgtgagattc
     3961 tttcccgggt tcagcggcac ggtcaccagc ggcaccgcga cgccgaggat ggtggtctgc
     4021 tgcgtctcga ggccggtgcg gtcgtattcg cgcgcggcat cccaatcttc gagctgcacg
     4081 acgacctcga tccgcttctg ctggcgtacc gcgcggatgc cgaacagctg cttgatgtcg
     4141 atcaacccga tgccgcggat ctccatgtga tggcgcgcca tctcgtggcc gcggccgatc
     4201 aggatgtcca ccccgcggcg cgtgacgtgc accaggtcgt ccgccaccag ccggtggccc
     4261 cgctccacca gatcgaggac gcactcgctc ttgccgatcc cggagcggcc catgaacagc
     4321 aatcccacgc cgtacacgtc ggcgagcgag ccgtgcagcg tcgtcgcggg tgcgaacacg
     4381 tcgtccaggt acggcttcag gcggcggtag aattcggccg tcttgagcga ggtgcgaatg
     4441 accggcacct gcctggtgcc cgccagctcg agcagctcgg ccggcgcctc ctgcgacttg
     4501 gtgatcacga cgcacggcag gtcgaacttg aagaagagct ccagggagcg gcgccgctcg
     4561 gcgggctcga gggagccgag gtagctgatc tcggtttcgc ccaggatgtg gatgcggtcg
     4621 cccacgaagc gcgccgtata ccccgcgagc accagtcccg gactcgacgc ctccgacgag
     4681 ggaatctcgc gatcgagtcc ttgctcgccg gtgagcgact cgagctcgag ggcgcccttg
     4741 cgcgccagga ggtcgcgaac gcggggccgc tggttcactc ggcctcgttt cgcgccaccg
     4801 agcgccgccg cgccggaccg gagcgcttgg cgggcgactt gtcgagctgg cggcgcaccc
     4861 gggccgccgc ccggtccagc gccgtgcggt ggtcgatccc ctcggcccga cccacgaaca
     4921 gggcgccgcg ggcggcatgg agccgcagct ccgccgcggc cacgccgtga ttctcgctga
     4981 agatcacctg accgtcgtgc ggccgggccg cgacccgtgc gagccgctct acgagggccc
     5041 gcgcccgcag ccgcagatcg gccgtcacgt cgcagtggcg ggcggtgacg gtgatgtcca
     5101 tgcattcacc tccaatgcgc gatgacccgt tcgagatggg cgccggtcag gtgggccgcg
     5161 cggtcccacg tcagcgggag ggcgaacgcc cgcgccgcga cccccagccg cgccacgagc
     5221 gcgggatccc gggccagctc cccgatgcgc cgcgcgagct cggccgcgtc gcggtgcggc
     5281 acgaggaacc ccgtctcacc gtggcggacc gtctcgcgca ggccgggact gtcggaggcc
     5341 agcgccggcg tcccgcaggc ggcagcctcg acgttcgaga taccccagcc ctccttgggg
     5401 ctggggaaga ccacagccca cgccgagcgc agcaggcggc gcttttcttc ctccgacacg
     5461 aaacccagga agcgcaccgc gtccatcagc gccagccgcc gcacctccgc ctccagccgc
     5521 gggcggtcgt cgcccgcgcc cgcgatctcg agcgtcgcgt ccaccccccc cgcatgcgcc
     5581 aacgccagcg cccgcaacgc gatatccact cctttataac gcttcaggcg gccgacgtac
     5641 aggaacgtgg gccgcgcggc acgcggcgtt gcggagtcgg ggtgatacca ggtcgcgtcg
     5701 atccccgggt agatcacctc gatccgctcg gcggctacgc cccgggccac cagatcgtcc
     5761 cgggtgctgt tgctgatgac atggaacgcg gcacgggcgt agacgcgcgg aatcgcccgc
     5821 tcggccgccc aaacgaccgc cgccaccggc cagctcgcct ccacaaacgc cgtcgtcccg
     5881 aagaggtgcg gcacgatggc gcagaacggc agatcggtga gcgacggcag gaacagcggc
     5941 aacttgttga cgtcctcgac caccacgtcg tatccatgcg gctgccgcaa cgccgcacga
     6001 acggcgccgc gcgccaggag cgcgaagctc aggcgaccgc ccacgcgacg cacgcggagg
     6061 ccgtccacct cggccgcggc cgccgcgccg gcccagccgc tggcgaccag gtccacgcgg
     6121 tgcccctgcc gggccagccg cccgaagatc tcgtgcaggt gtatctccgc gccgccggcg
     6181 tgcggattct cacggtcgtt ccagttgacc agcaggatcc tcacaaccgg cccagctccc
     6241 gcgccaccgc atcgagcacg ccgttgacga atgccggcgc gcgccccccg ccgaaccagt
     6301 gggccagctt caccgcctca tccatcacga cccgcgccgg cgtactgcct tcgtcgagct
     6361 ccgccatcgc gagacgcagg atgttcttct cgaccacgcc gacgcgctcc agccgccagt
     6421 gctcggagag ccgcgacagc tgcgcgtcgt actccgcctg ccccgccacc gcccgcgctg
     6481 caagctccgc accgcggtcg tagcccacgg ggatcccccc gtaggtcctg gcgagacgcg
     6541 tgacgacggt atcgatcgag ggatgccccg acaggtccca ggcgtagagc agctgcagcg
     6601 cgcgggcgcg cgccttagtc tcgggccgtt ccacggcgca cccggtgcag gacgtccgcc
     6661 gtctggagcg ccgccgccgc ggcctcgtag cccttgttgc cggcggcgcc gccggccctc
     6721 gcttccgcct gcgcctgatt ctcggtcgtc aacacgccga agccgagggc gacccggtgc
     6781 gccagcgcga cgttgcccaa cccgcgtgcc gcttctcccg ccacgtactc gaagtgcggc
     6841 gtctcgccgc ggatcacgca ccccagcgcc acgatggccc ggaagcggcc ggagaccgcc
     6901 gcctcctcgg ccgcgaccgc cagctcgaac gcgcccggca cccagaactc ctcgacgtgc
     6961 gagtcgggaa ctcccgcttc acgcaacgcg tcccgggccc cgtcgagcag gcgccgggtg
     7021 atcagctcgt tgtagcggct gacgacgatg gccacgcgcg gatgctgcgc cacggtggtc
     7081 agtgggtgag caggtggccg agcttgtcgc gcttgacgtc caggtaggcg cggttctcgt
     7141 cggagggcg
//
LOCUS       DBNGZW010000649         7169 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1006790, whole genome shotgun sequence.
ACCESSION   DBNGZW010000649 DBNGZW010000000
VERSION     DBNGZW010000649.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1006790"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="AC16JC_15235"
     CDS             <1..475
                     /locus_tag="AC16JC_15235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166102.1"
                     /translation="APVLAVAGFIAAIVPDTDPDEREVTIPAGATLVAVLADPLSTAR
                     SQPGDEVELRTVEPLHVRGADDIPAGIVIHGTVLEAAAGDQAAESPGISVRFNEMEID
                     GDEHAIATEEYRFGTLVERATNRHLLLPAGERMNIRLTRPVTVEYHLAARAQSTE"
     gene            519..857
                     /locus_tag="AC16JC_15240"
     CDS             519..857
                     /locus_tag="AC16JC_15240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008906408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166103.1"
                     /translation="MGSRRGPPARHHVYVIELSRDVLRHAKFRRANPHHDPAKPCLYV
                     GMTGLDPETRFERHRYGVKDNPYVRRYGLRLRPDLYAELNPLSYAAAQRMERVLAGEL
                     RADGYAVWQK"
     gene            1019..2536
                     /locus_tag="AC16JC_15245"
     CDS             1019..2536
                     /locus_tag="AC16JC_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014266191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HIR5166104.1"
                     /translation="MTRRLPDHPDFDHLKKQAKQLLRGLESRDPQALERYHATLSRPA
                     PAEPKLADAQHALAVEYGFTSWPKLKAHVEGIGASADPAAALVAAIKANDPAAARRVL
                     HDHPGLKARLDEPLPGFSFGATPLLAALPAGNRELIDLLLEAGADINQRSHWWAGSFG
                     VLDDDHGLAPYLIERGARVDVHAAARLGMMDRLAGLIAADPELVHARGGDGQTPLHFA
                     PTIEVAAFLLDQGADIDAHDVDHESTPAQYMVRSRQDVARYLVRRGCKTDLLLAAALG
                     DVDLVRRHLDENPECIRVSVSDRWFPKRDPRSGGCIYIWTLGGHKTAHVVAREFGHED
                     VLRLLVERSPEELLLGLACELGDETQFRNLLAKRPDLSRTLSEDDKRKLVNAAESRNN
                     NAVRLMLAAGWPLDARGQSGGTALHWPSWHGNLEMVREILRYHPAVDVRGDDYDMPPV
                     GWALHGSENSWHRETGDYVGVVEALLAAGAKVPPLTGDMVVSASIMEVLRQRHFM"
     gene            complement(2589..4685)
                     /locus_tag="AC16JC_15250"
     CDS             complement(2589..4685)
                     /locus_tag="AC16JC_15250"
                     /inference="COORDINATES: protein motif:HMM:NF021942.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008239 - dipeptidyl-peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070009 - serine-type aminopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HIR5166105.1"
                     /translation="MKPYLLLITLPLTLAAQQPADTIRAGRYDNGKMWTFEYPPDRYF
                     TETYGFAADSAWFSRARLAALRIPGCSASFVSPHGLVVTNHHCSREGIVAVTKPGETL
                     LDSGFVAHALAEERRMPDFFADQLLAVQDVTDQIVPQLDRAPNDSVRDAWRREASLVV
                     QGTLRTQYAHPGDSIWVQVVGLYNGGRYSAYVFRRFTDVRLVAAPELQMGFFGGDPDN
                     FTYPRYALDFSVIRIYGTDGKPYETSSWFRWGSDGVKPGDPVFVIGNPGSTSRLKAVS
                     QLEYERAVSVPAQVRFLDSRLQALHAFYDAHRATGDSLDMRNWMFGVSNSLKSSQGRL
                     GALLDPAILARRRDAERQLGESLAVHRELKDRYGRLLERMADVQRRKATLAAPYRAFF
                     YFGNGFAGSALSRRALNAARIAGATTDSAAVFSRRARAVGNLPPALEKSFLALELGDI
                     VRAYGPAHSLTTAALMGQPTPDAAADLLLRTSALADSTRADRALSTGLSSDDPALRLV
                     GALLPALQAFNRDFAAVAAEERGLASDLGRARFAVYGRNVPPDGTFSPRIADGVVQGY
                     AYNGTLAPPFTTFFGMYDRFRANGTGSEWDLPYRWRTPPAGLDLGTPLDFVSTADTYG
                     GNSGSPVVTKDLRVVGLNFDRNIDALVRDYIYLPERGRNVMVDVRAIQAALANVYGAR
                     RIVTELTTGRLGESDR"
     gene            4860..6716
                     /locus_tag="AC16JC_15255"
     CDS             4860..6716
                     /locus_tag="AC16JC_15255"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5166106.1"
                     /translation="MRRHGPPSRLSPSEKPPTWAQRIEALRHVPPLLRLVFQTHRGYT
                     VAILSLRIVRSFIPVAVLWIGKLIIDGVIAAIAVAHAGNPVDWWTLGGLIGLELGIAV
                     VGEALARASSLLESLLGDLFANRISVRLMRHAATLDLAQFEDPDTYDHLERARRQTVG
                     RIGLFALLLSTAQDLVTLASLAGVLLVQLPWLLLLLALAVVPSFLGEAHYASLGYSLL
                     FRWTPERRLLDYLRYMGASDESAKEVKLFGLSDFLVGRYAKLSDEFYLANKKLAVRRN
                     IVSTALVTLGTLGYYAAYAVIIYLTVLGRYTIGSLTFLAGSFRSSRDLIQRILLSLSN
                     IYEQSLYLGDLFTFFDVQPLVVSKPGGRAVPKPIEKGFEFRDVGFRYPGSERWAVRHL
                     TFTVGPGERIALVGENGAGKTTLVKLLARLYDPDEGQILLDGVDLRDYDLTSLRRNIG
                     VIFQDFVRYDFILKENIGVSEIERLADEARIEEAARRSHADALAGRLAGGYGQMLGRR
                     FEGGVDLSGGEWQKVALARAYLREAQVLILDEPTAALDARAEYEVFLRFAELTKGRMA
                     VLISHRFSTVRMADRILVLQGGELVDQGTHDELVARGGLYAELFSLQAAGYR"
     gene            6733..>7169
                     /locus_tag="AC16JC_15260"
     CDS             6733..>7169
                     /locus_tag="AC16JC_15260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166107.1"
                     /translation="MYTTCIFCHRPLGSNETVEQFPVGRRLAFDAARGRLWVVCRGCE
                     RWNLSPLEIRWEAIEECDRLFAITRLRVSTDNIGLARLADGLELVRIGEPQRPEFAAW
                     RYGDQFGRRRRRAFLIGGGIAVAVGAAVVGASAAGVGLGGFGA"
BASE COUNT         1109 a         2461 c         2503 g         1096 t
ORIGIN      
        1 ggcccccgta ctcgcagtgg ccggtttcat cgccgcgatc gtacccgata ccgatccgga
       61 cgagcgggaa gtgacgatcc ctgccggcgc gacgctcgtc gcggtgctgg ccgatccgct
      121 ctccaccgcc cgcagtcaac ccggcgatga ggtcgagctc cgcacggtcg agccgctgca
      181 cgtgcgcggc gccgatgaca tccccgccgg gatcgtgatc catggcacgg tcctcgaggc
      241 cgccgcgggc gaccaggcgg cggaatcgcc ggggatcagt gtgcgcttca acgagatgga
      301 gatcgacggc gacgagcacg cgatcgcgac cgaggaatac cgcttcggca cgctcgtcga
      361 gcgggccacg aacaggcacc tgctactgcc cgccggggaa cggatgaaca tccggctgac
      421 ccgccccgtg accgtcgaat accatctggc agcgagggca cagtcgacgg agtagtccag
      481 aagagccgca ccggcggtcc gggtatcttc ccggatccat gggatcacga cgcggaccgc
      541 cggcgcgcca tcacgtctac gtcatcgagc tgtcgcggga cgtcctgcga cacgccaagt
      601 tccgccgcgc caacccgcat cacgaccctg ccaagccctg cctctatgtg ggaatgacgg
      661 gactcgatcc cgagacccgg ttcgagcggc accgctacgg cgtgaaggac aacccctacg
      721 tgcggcggta cggcctccgg ctgcggcccg acctctacgc cgagctcaac ccgctctcct
      781 atgcggcggc ccagcggatg gagcgggtgc tggcggggga gctgcgggcg gacggatatg
      841 cggtgtggca gaagtgaacc ttgtcgcgcc ggtggcggcg caataaactg gacggtgcaa
      901 tgcaggttgc atcccgtccg ctggaaggga ccatcccacc tgcgcatcct cgcagtacac
      961 aatcagcctc tcttcggccc aagccggcgg acattgggcg ctcgcgaagg gaggagtcat
     1021 gacccgccgg ctgccggacc atcccgactt cgatcacctg aaaaagcagg cgaagcagtt
     1081 gttgcgcggc ctcgagtcgc gcgacccgca ggcgctcgag cgttaccacg ccaccctctc
     1141 ccgcccggct cctgccgagc ccaagctggc cgatgcccag cacgcgctcg ccgtggagta
     1201 cggcttcacg agctggccga aactcaaggc gcacgtggaa gggatcgggg cctcggccga
     1261 ccccgccgcc gcactcgtgg ccgccatcaa ggcgaacgac cccgcggccg cgcgccgtgt
     1321 cctgcacgac caccccgggc tcaaggcccg gctcgacgag ccgctccccg gattctcttt
     1381 tggggcgacc ccactgctcg ccgcccttcc tgcgggcaac cgggagttga tcgacctgct
     1441 gctcgaagcc ggcgcggaca tcaaccagcg cagccactgg tgggccggga gcttcggcgt
     1501 cctggacgac gaccatggcc tcgcgcccta cctgatcgag cgcggcgcgc gcgtggacgt
     1561 ccacgcggcg gcccggctcg gcatgatgga ccggctcgcc gggctgatcg ccgccgatcc
     1621 cgagctggtg cacgcgcggg gaggtgacgg gcagacgccg ctgcacttcg caccgacgat
     1681 cgaggtcgcg gccttcctgc tggaccaggg cgcggacatc gatgctcacg acgtggacca
     1741 cgaatcgacg ccggcccagt acatggtgcg cagtcggcag gacgtcgcgc gctacctggt
     1801 gcgccgcggc tgcaagaccg acctgctcct ggccgcagcg ctgggcgacg tggatctcgt
     1861 gcgccggcat ctggacgaga atcccgaatg catccgcgtg agcgtctcgg accggtggtt
     1921 tcccaaacgg gacccgcgca gcgggggctg catctacatc tggacgctgg gcgggcacaa
     1981 gaccgcgcac gtcgtcgccc gtgaattcgg tcacgaggac gtgctgcgcc tgctggtgga
     2041 acgcagcccc gaggagctgc tgctcggcct ggcctgcgag ctgggcgacg agacgcagtt
     2101 ccgaaacctc ctggcgaagc gccccgacct gtcgcgcacg ctcagcgagg acgacaagcg
     2161 caagcttgtc aatgcggcgg agagccgcaa caacaacgcc gtccgcctga tgctcgcggc
     2221 gggatggccg ctcgacgcgc gcggccagag cggcgggacg gcgctgcact ggccatcctg
     2281 gcacggcaac ctggagatgg tgcgggagat cctgcgctac cacccggccg tggatgtgag
     2341 gggggatgac tacgacatgc ccccggtcgg ttgggcgtta cacggctccg agaacagctg
     2401 gcacagggaa acaggggact acgtcggcgt ggtggaggcg ctgctggcgg cgggggccaa
     2461 ggtgccgccg ctcaccggcg acatggtggt gagtgcatcg ataatggagg tacttcgcca
     2521 acgtcacttc atgtgacaga tcagccgagt cgcgacttca gtcgcgaaca acgtgcgacg
     2581 catccccgtt atcgatcgct ctccccgagc cggcccgtcg tcagctcggt gacgatccgc
     2641 cgcgccccgt acacgttcgc gagcgccgcc tggatcgcgc gcacgtccac catcacgttg
     2701 cggccgcgct cggggagata gatgtagtcg cgcaccaggg cgtcgatgtt gcggtcgaag
     2761 ttcaagccga ccacgcgcag gtccttggtg acgaccgggc tccccgagtt gccgccgtac
     2821 gtatcggcgg tcgagacgaa gtcgagcggc gtccccaggt cgaggccggc gggcggcgtg
     2881 cgccagcgat aggggagatc ccactcgctg ccggtcccgt tggcccggaa gcggtcgtac
     2941 atcccgaaga acgtggtaaa cggcggggcg agcgtgccgt tgtaggcgta gccctgcacg
     3001 acgccgtcgg cgatgcgcgg tgagaaggtg ccgtcgggag gcacgttccg cccgtagacg
     3061 gcgaaccggg cgcgccccag gtcgctcgcc agcccgcgct cctcggcggc gacggcggcg
     3121 aagtcccgat tgaaggcttg cagcgcgggc agcagcgcgc ccaccaggcg caatgccgga
     3181 tcgtcgctcg agagcccggt ggagagtgcc cggtctgccc gggtcgagtc ggcgagcgcc
     3241 gaggtgcgca gcagcaggtc cgcggccgcg tccggcgtcg gctgccccat gagggcggcc
     3301 gtggtgagtg agtgagccgg accataggcg cgcacgatgt cgcccagctc gagcgccagg
     3361 aagctctttt cgagcgccgg cggcaggttg ccgaccgccc gcgcgcgccg gctgaacacc
     3421 gccgccgaat ccgtcgtggc accggcgatg cgcgccgcgt tcagggccct ccgggagagt
     3481 gccgatcccg cgaagccgtt cccgaaatag aagaacgcgc ggtagggcgc cgccagcgtc
     3541 gctttgcgac gttgcacgtc cgccatccgc tcgagcagcc tgccgtagcg gtccttcagc
     3601 tcgcgatgta ccgcgagact ctccccgagc tgccgctcgg cgtcacgccg acgcgccagg
     3661 atggcgggat cgagcagcgc gccgagacgt ccctgggagg acttcaggct gttggacacg
     3721 ccgaacatcc agttgcgcat gtcgagcgag tcgcccgtgg cccggtgcgc gtcgtagaac
     3781 gcgtggagcg cctgcagccg ggaatcgaga aaccggacct gggccggcac gctcaccgcc
     3841 cgctcgtact cgagctgcga gacggccttg aggcgcgagg tcgagccggg gttgccgatc
     3901 acgaagaccg ggtcaccggg cttcacgccg tccgagcccc accggaacca gctggaggtt
     3961 tcgtacggct tgccgtccgt gccgtagatc cggatcacgg agaagtcgag cgcgtagcgc
     4021 gggtaggtga agttgtcggg atcgccgccg aagaagccca tctggagctc gggggcggcg
     4081 acgaggcgga cgtcggtgaa gcggcggaac acgtaggcgg agtagcggcc gccgttgtac
     4141 agccccacga cctgcaccca gatcgagtcg ccggggtgcg cgtactgcgt ccggagcgtg
     4201 ccctgtacca cgaggctcgc ctcgcgccgc cacgcgtcgc gtaccgagtc gttgggcgcg
     4261 cggtcgagct gcggcacgat ctggtcggtg acgtcctgca ccgccagcag ctggtcggcg
     4321 aagaagtccg gcatgcgccg ctcttcggcc agagcgtgcg cgacgaatcc cgaatcgagc
     4381 agcgtttcgc ccggcttcgt caccgcgacg atcccctcgc gggagcagtg gtgattggtg
     4441 accacgagcc cgtgcggcga gacgaagctg gccgagcagc ccgggatgcg gagtgcagcg
     4501 agccgggcgc ggctgaacca ggcggagtcg gcggcgaagc cgtaggtctc ggtgaagtag
     4561 cggtcgggcg ggtactcgaa cgtccacatc ttgccgttgt cgtaccggcc ggcgcgaatc
     4621 gtgtcggccg gctgctgggc agccagcgtc agggggagcg tgatcaggag gaggtacggt
     4681 ttcatggaga ctccacttga gaactctcgc cttgcgtccc gggaccttgc ccccgcaggg
     4741 accgcggccc gccggcccct cgaatcctcc ccgcgcccgg ttgctcaggc atcgtgcgag
     4801 caacgctgca ccaccggaac acaagacgca aggaggcggc tacttttcac gccgtgagcc
     4861 tgcgacgaca tggtccccca tcgcgcttgt ccccaagcga gaagcccccc acctgggccc
     4921 agcgcatcga ggcgctgcgc cacgtaccgc cgctgctgcg cctcgtgttc cagacgcacc
     4981 gcggctatac cgtcgcgatt ctctcgctcc gcatcgtccg ctcgttcatt ccggtggcag
     5041 tcctgtggat cggcaagctc atcatcgacg gcgtgatcgc ggccatcgcc gtggcgcacg
     5101 ccggcaatcc cgtggactgg tggacgcttg gcggactgat cggtctcgag ctgggcatcg
     5161 ccgtcgtggg cgaagcgctc gcccgtgcct cgtcgctgct cgagagcctg ttgggcgacc
     5221 tgttcgccaa ccgcatcagc gtgcggctca tgcggcacgc cgccacgctc gacctggctc
     5281 agttcgagga ccccgacacc tacgatcacc tcgaacgggc acgccgccag acggtgggtc
     5341 gcatcgggct cttcgccctg ctgctctcga ccgcccagga cctggtgacc ctcgcctcgc
     5401 tcgccggggt gctgctcgtg cagctgccct ggctactcct gctcctcgcc ctggccgtgg
     5461 tcccgtcgtt cctgggagag gcgcattacg cgtcgctcgg ctactccctg ctgttccgct
     5521 ggaccccgga gcgccgcttg ctcgactacc tgcgctacat gggtgcctcc gacgaatcgg
     5581 cgaaggaagt gaagctgttc gggctctcgg atttcctggt ggggcgctat gcgaagctct
     5641 ccgacgagtt ctatctggcg aacaagaagc tcgccgtgcg gcgcaacatc gtgtcgacgg
     5701 cgctggtgac gctcgggacg ctcggctact acgccgcgta cgcggtcatc atctacctca
     5761 cggtgctggg ccgctacacc atcgggtcgc tgaccttcct cgcgggctcg ttccgctcga
     5821 gccgcgacct gatccagcgc atcctgctct cgctgtcgaa catctacgag cagagcctct
     5881 acctgggcga tctgttcacg ttcttcgatg tgcagccgct cgtcgtatcg aagccgggag
     5941 gccgggcggt accgaagccg atcgagaagg gattcgagtt ccgcgacgtc gggttccggt
     6001 acccgggatc ggagcgctgg gcggtgcgcc atctgacctt cacggtgggt cccggggaac
     6061 ggatcgcgct ggtcggtgaa aacggtgccg ggaagaccac cctcgtgaag ctgctcgccc
     6121 ggctgtacga ccccgacgag ggtcagatcc tcctcgacgg cgtggacctc cgcgactacg
     6181 acctcacgag cctgcgccgc aacatcggcg tgatcttcca ggacttcgtg cgctacgatt
     6241 tcatcctgaa ggagaacatc ggggtgagcg agatcgagcg gctggcggac gaggcccgga
     6301 tcgaggaagc ggcacgccgc agccatgccg acgccctggc cgggcggctc gccggcggct
     6361 acggccagat gctgggccgc cgcttcgaag gcggcgtgga tctctcgggt ggcgagtggc
     6421 aaaaggtcgc gctggcgcgc gcctatctgc gtgaagccca ggtgctgatc ctggacgagc
     6481 ccaccgcggc gctcgacgcc cgcgccgagt acgaggtgtt cctgcggttc gcggagctga
     6541 cgaaggggag gatggcggta ctcatttcac accgcttctc gacggtgcgc atggccgacc
     6601 gcatcctggt gttgcagggc ggtgagctgg tggatcaggg cacacacgac gagctggtag
     6661 cgcgcggcgg cctctacgcc gagttgttca gcctccaggc cgccgggtat cgctgagcct
     6721 caggggagac gcgtgtacac cacctgcatc ttctgccatc gaccgctcgg ctcgaacgaa
     6781 acggtggagc aattcccggt cgggcgccgc ctcgcgttcg atgccgcccg gggacgcctg
     6841 tgggtcgtgt gccgcggctg cgagcggtgg aacctcagcc cgctcgagat ccgctgggag
     6901 gcgatcgaag agtgcgaccg gctcttcgcc atcacccgcc tccgcgtgtc caccgacaac
     6961 atcgggctgg cccggctcgc cgacggactc gagctggtcc ggatcggtga gccgcagcga
     7021 ccggagttcg cggcgtggcg gtacggagac cagttcggtc ggcggcggcg gcgcgccttc
     7081 ctgatcggcg ggggcatcgc ggtcgccgtc ggggccgcgg tcgtcggcgc ttcggcggcc
     7141 ggggtcgggc tcggcgggtt cggcgcctt
//
LOCUS       DBNGZW010000650         9221 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1903433, whole genome shotgun sequence.
ACCESSION   DBNGZW010000650 DBNGZW010000000
VERSION     DBNGZW010000650.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 9221)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9221)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9221
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1903433"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..680
                     /locus_tag="AC16JC_15265"
     CDS             <1..680
                     /locus_tag="AC16JC_15265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HIR5166108.1"
                     /translation="PDTLSAAIWTSPVLPGSVELPKGADVQVYEIRPAVVTIQLDSVS
                     SKEVPILPRFTVVAESGQTFEGGLQITPSVARLVGPDRLLSRIEAVATLPTAFDAVSG
                     PFTRVVQLDTAPLGIVRISPHDVRVSGSTTPVYERIFNLLPVESGAGPLTGYELRPPR
                     ATVVVRGPEALIQALTKDSLKVIVHLTGAVTDTATVRVTVLAPRGVTARAVPDSVLVV
                     RRRGGRG"
     gene            673..1734
                     /gene="tsaD"
                     /locus_tag="AC16JC_15270"
     CDS             673..1734
                     /gene="tsaD"
                     /locus_tag="AC16JC_15270"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003465345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HIR5166109.1"
                     /translation="MADVVLGIETSCDETSAAVLVGGAGGRPPELASLVILSQDVHQV
                     FGGVVPELASRAHLAALPAVVERARAEAGVSWNAVDAVAVTAGPGLVGALLTGVVYAK
                     ALAYAGDRALIGVNHLEGHLFAPAVEDPELDPPFVALLVSGGHTLLLDVPAWGRYRLL
                     GATRDDAAGEAFDKVATLLELGYPGGPAIEKLAATGDPARFRFPRPMRDEGFEFSFSG
                     LKTAVRHAVRNSTDLARDRADLARGFQDAALDVLVTKLARAVAATRYTTAVLGGGVAC
                     SRALSALAAERLAGSARVAVASPRLNTDNAAMIARAGWWRLARGERSDWTLDARADLP
                     IPGLEHPSPVPRPSSPRAS"
     gene            1731..2582
                     /locus_tag="AC16JC_15275"
     CDS             1731..2582
                     /locus_tag="AC16JC_15275"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR5166110.1"
                     /translation="MTVYPLVLRIGSFSITGFGLMMMLAFILAGWAMQLELRRRGLDE
                     EYAADVVIGAVLGGLIGAKLWYVALTGESLFSRGGFVWYGGFLGGVLGVVFNSWRRRV
                     PIAFTAELVAPALTLGYAVGRVGCFLVGDDYGVPSSLPWAVKFPDGLPPTTIGNLAQF
                     HVAFPPGAAPTQVVAVHPTQVYETALMLLATAWLWKRRDHPHAMGWLFGCYLVIAGVE
                     RLLIEFLRAKDDRVLHGFTIAQAASVALIAAGIVLVNRLKQPSAVSAQPSALMKRHAA
                     PAGQGDA"
     gene            complement(2518..4242)
                     /locus_tag="AC16JC_15280"
     CDS             complement(2518..4242)
                     /locus_tag="AC16JC_15280"
                     /inference="COORDINATES: protein motif:HMM:NF015213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HIR5166111.1"
                     /translation="MSVARRIVAAAQLAAKEYALGVPVQGGRVTDAEEVKEAGLFIQQ
                     ARLDVHVLPADTRAYADSTLGAMAALLDRTAAPAQVSGLAEALAGRLARSSGDLFPVP
                     VVAPSLARGAELYVEHCASCHGATGRGDGPGARGLTPPPANLADPRVMGAKSRVDLYR
                     QLLLGVPGTAMPTFEHTLSEDDRWAVAAYALTLQYGGSQAAAIMAVVRRQLDSAVSQG
                     SDRLAFDAYLTFEGVETDVRAHDPGLATRLEGEFARLRSRAAAGAPPAELRVLQRALL
                     GDLEHAERLLVDRASGANLFLQSLMLLVREGFEAILIIAALMSFLTKAGAPARRREVA
                     LGAWAAVAASGITAVLFELLLNVPPVRRSALEGFTMLAAVAVLFYVSYWLLSKIEADK
                     WSAFLKGKMQAALSSGSALALASVAFLAVYREGVETILFYKALLASGGTGGAGAVVAG
                     VALGAACLVLLYVLLMRLGVRIPMKAFFAVTGALLYYMAFVFAGKGIAELQEAGMVGT
                     TVLPSLEWLRVPFLGIYPTVQSLALQSVLILLLLVALIAKLRPSAVRRHPDPREPHVV
                     SSGPTAER"
     gene            complement(4376..4762)
                     /gene="acpS"
                     /locus_tag="AC16JC_15285"
     CDS             complement(4376..4762)
                     /gene="acpS"
                     /locus_tag="AC16JC_15285"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR5166112.1"
                     /translation="MSVVGVGVDLVEVERVRRMLAEKGTHVYDRLLTPGEAAYCRSKT
                     DPAESVAARLATKEAVYKALQGSPAARGIGWREIEVVRDEDGRPDVVLTGLAAQRASE
                     LGVSRIFVSLSHTHAAAVATAVVEAV"
     gene            complement(4759..6942)
                     /locus_tag="AC16JC_15290"
     CDS             complement(4759..6942)
                     /locus_tag="AC16JC_15290"
                     /EC_number="3.4.17.-"
                     /inference="COORDINATES: protein motif:HMM:NF012471.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HIR5166113.1"
                     /translation="MARRRAGILILALAAAPAASAAGQLRPLAVRVSGPAGLDSLARL
                     GFEVAGARVVAGGLEALVVASPETERALATRGFLAAPPVGIPAAGPAGDSFAVFRSFD
                     RPGTGIRATLAAWAAADTLIHVDSIGASVEGRPILAVKIGPAADAPGRPNVLFLGTHH
                     AREWVSTEVAMALIRWLADSAAALTASRDVWVIPVENPDGYQFTFTTDRYWRKNRRLN
                     GDGSYGVDLNRNYPAFWAYDEAGSSGLTGAETYRGPAPASEPETRAVIAFHAAHPPVV
                     SVSYHTYSGLVIYPYGYLPGAFAPDVPLFRALAGTDLAPAVRDSVPASFHSYYHPGPG
                     WNLYPTNGEYTDWAYRAHGTIAFTPELTSGCCTPDSGLYYGFAFPDDSSLVRRVFRDN
                     LPFALAVIAAAGDPALAAGPTGAVASPPRIETIWPEARVSLSSSDPQPFALSVRGAGG
                     IIVQRSLTADSLSRGTLRTVWHATLSGDRPAALRVDDAGRGAGPTAELLSLAGAEDGD
                     LGWRGWQRSTDAVAGSWSWTTSIGALDTLISPVVDLGGRTRLWLQFWTRHRGSTYTPE
                     QHGLVQVSADSGSTWTTVADVVGDGPAWYPMRLDLPMLTGARGARVRFITVGFTWWVD
                     AVGFASDSTAAFLSPAPPAALALSENPVRGNQVWISWPAGAGAPRLGIYTFTGERLIS
                     TTLPAGTIEYAWDLTAGSHRIANGAYLVVLELDGRVSRRRVFVAR"
     gene            complement(6915..7643)
                     /locus_tag="AC16JC_15295"
     CDS             complement(6915..7643)
                     /locus_tag="AC16JC_15295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166114.1"
                     /translation="MRAEMAVTLPVPELGPPLGRVIVPHRLSDPWVPLDDVREALATR
                     VMECAGSARRDAADAAPARALRQLDRDAWSEAWNEAVRRAAERVAQGLDAALALEARR
                     VRMPARRSRQLPLSPGERRAIAARLASGAEGFEAALEDVARATEALHRDWPGDAEMIA
                     AWRATQELAARRLEAAWLALETLVDQERARWAPEVDAIRAWRPSLMPVFAVWLPLAAV
                     VVWLALVLGGYLSAPAWLAGALGF"
     gene            complement(7640..8242)
                     /locus_tag="AC16JC_15300"
     CDS             complement(7640..8242)
                     /locus_tag="AC16JC_15300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166115.1"
                     /translation="MSAPRRTGNGPRPNAELEVKARVTNVAALRRALRRAGARLEFRG
                     RMSDRRYDHARELAHRDDVLRLRVYRPDGRGAGHAVLAWKGAASTRPGRRGGYRLRAE
                     LEIAVADPDAMDLLLGRLRYRVSMKIDRRIEVYRLGAAMLRLERYPRMDTLLEVEGPP
                     AAIERAVAATGLPRERFLAESLPHFVTAYRRRTGRKARLA"
     gene            complement(8239..8937)
                     /locus_tag="AC16JC_15305"
     CDS             complement(8239..8937)
                     /locus_tag="AC16JC_15305"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5166116.1"
                     /translation="MRLVLFDIDGTILLTDGAGRRAIHRALLELVGTAGPIGDHRFDG
                     KTDPQIVRELLSLAGHGDTGMQERIQAVCARYLELLEDELTRPSQTRVMAGVRELLTA
                     LAGPEAAGAALVGLLTGNLERGAALKLRSAGIDPARFAVGAYGSDAPDRPLLPAIART
                     RAESLTGRTIAGADVIVIGDTPDDVACARPIGARTVAVATGHYDVGSLAAAGATHVFA
                     DLSDTAAVLQALLA"
     gene            complement(8937..>9221)
                     /locus_tag="AC16JC_15310"
     CDS             complement(8937..>9221)
                     /locus_tag="AC16JC_15310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HIR5166117.1"
                     /translation="YAARGVALPRDSDQQFTVGSEIPPAPAGEGYEKGNLLFFAERGR
                     VSHVALWAGAGRIVHSTLSCGGVVSENFLADVPRLQRLRDQVVGVRRVES"
BASE COUNT         1361 a         3402 c         3246 g         1212 t
ORIGIN      
        1 tgccggacac gctgagcgcc gccatctgga ccagcccggt gctgccgggg agcgtcgagc
       61 tgcccaaggg ggcggacgtc caggtctacg agatccgccc ggcggtcgtc accatccagc
      121 tcgattccgt gtcgagcaag gaggtcccga tcctgccgcg cttcacggtc gtcgccgagt
      181 cgggccagac cttcgagggt gggctgcaga tcacgcccag cgtcgcccgg ctcgtcggcc
      241 ccgaccggct gctgtcgcgg atcgaggcgg tcgccacgct ccccaccgcg ttcgatgcgg
      301 tgagcggccc cttcacccgg gtcgtccagc tcgacacggc gccgctgggc atcgtgcgca
      361 tctcgccgca cgacgtccgc gtcagcggat cgaccacccc ggtgtacgag cgcatcttca
      421 atctgctccc ggtggaaagc ggggccggcc cgctcaccgg ctacgagctg cgaccgccgc
      481 gcgccaccgt ggtcgtgcgc gggcccgagg ccctgatcca ggccctcacc aaggacagcc
      541 tcaaggtcat cgtccacctg accggtgccg tcacggatac cgcgaccgtg cgggtgacgg
      601 tgctcgcccc ccgcggcgtc accgcgcgcg ccgtgccgga ttccgtcctc gtggtgcgac
      661 gccggggcgg ccgtggctga cgtcgtcctg gggatcgaga catcctgcga cgagacctcc
      721 gcggcggtgc tcgtcggcgg ggccggcggg cggccgccgg agctcgcgag cctggtgatc
      781 ctgtcgcagg acgtgcacca ggtgttcggc ggcgtggtgc ccgagctggc aagccgtgcc
      841 cacctcgcgg cacttccggc ggtggtggag cgcgcccgcg ccgaagcggg cgtgtcatgg
      901 aacgccgtgg acgcggtcgc ggtcacggcc gggcccgggc tcgtcggcgc gctgctcacc
      961 ggcgtggtgt acgccaaggc gctggcctac gccggcgatc gtgcactgat cggcgtgaat
     1021 catctcgagg gacacctgtt cgccccggcc gtggaggatc ccgagctcga cccgcccttc
     1081 gtggccctgc tcgtctcggg cggccacacg ctgctgctcg acgtccccgc ctggggccgc
     1141 taccggctgc tcggcgccac gcgcgacgac gcggccggcg aggcgttcga caaggtcgcg
     1201 accctgctcg agctggggta cccgggcggc ccggcgatcg agaaactggc ggcgacgggc
     1261 gaccccgcgc gcttccggtt cccccgcccc atgcgggacg agggcttcga gttctcgttt
     1321 tccggactca agaccgcggt gcgccatgcg gtgcgcaaca gcaccgacct ggcgcgcgat
     1381 cgcgccgacc tcgcgcgcgg gttccaggac gcggccctcg acgtgctggt cacgaagctc
     1441 gcccgcgcgg tggcggcgac gcggtacacc accgcagtgc tgggcggcgg ggtcgcgtgc
     1501 agccgcgccc tctcggccct ggccgccgag cggctggcgg gatccgcacg cgtggccgtc
     1561 gccagcccgc ggctcaacac cgacaacgcc gccatgatcg cgcgcgccgg ctggtggcgg
     1621 ctcgcgcgcg gcgagcgcag cgactggacc ctcgacgccc gcgcggatct gcccattccc
     1681 ggattggaac acccatcccc cgtcccccgt ccctcatccc ctcgggcgtc atgaccgtct
     1741 acccgctcgt cctgcggatc ggcagttttt cgatcaccgg cttcggcctg atgatgatgc
     1801 tggccttcat cctcgccggc tgggcgatgc agctcgagct gcggcgccgc gggctggacg
     1861 aggagtacgc cgcggacgtg gtgatcggcg cggtgctggg cgggctgatc ggcgccaagc
     1921 tgtggtacgt cgccctcacc ggggaatcgc tgttcagtcg cgggggcttc gtgtggtacg
     1981 gcggcttcct gggcggcgtg ctgggggtcg tgttcaacag ctggcggcgg cgcgtgccga
     2041 tcgcgttcac cgccgagctc gtggcgccgg cgctgaccct gggctatgcc gtcggccggg
     2101 tgggatgctt tctcgtgggc gacgactacg gcgtgccgtc gtcgctgccc tgggcggtga
     2161 agttccccga cggcctccct cccacgacca tcggcaacct cgcgcaattc cacgtggcgt
     2221 tcccgcccgg cgccgctccg acacaggtgg tcgcggtgca tcccacgcag gtgtacgaga
     2281 cggccctgat gctgctggcg acggcctggt tgtggaagcg ccgggaccat ccccacgcca
     2341 tggggtggct gttcggctgc tacctcgtga tcgcgggggt cgagcggctg ctcatcgagt
     2401 tcctgcgggc caaggacgac cgcgtgctgc acgggttcac gatcgcgcag gcggcgagtg
     2461 tggcgctgat cgccgcgggc atcgtcctgg tgaatcgatt gaagcagccg tcagccgtca
     2521 gcgctcagcc gtcggccctg atgaaacgac atgcggctcc cgcgggtcag ggtgacgcct
     2581 gacggctgat ggcctgagct tggcaatcag agcgaccagc agcagcagga tcaagaccga
     2641 ctgcagggcg agcgactgca ccgtcgggta gatccccagg aacggcaccc tcagccactc
     2701 gagcgacggc agcaccgtcg tccccaccat tcccgcctcc tgcagctcgg cgattccctt
     2761 ccccgcgaac acgaacgcca tgtagtagag cagcgcgccc gtgacggcga agaacgcctt
     2821 catgggtatg cgcacgccga gacgcatcaa caggacgtag agcagcacca gacacgcggc
     2881 gcccagcgcc actccggcga cgaccgcgcc ggcgcccccc gtgccgcccg aggcgagcaa
     2941 cgccttgtag aagaggatcg tctccacacc ttcccggtaa acggccagga atgccaccga
     3001 cgccagcgcc agcgcggagc ccgacgagag cgccgcctgc atcttgccct tcaggaacgc
     3061 gctccacttg tcggcttcga ttttggacag cagccagtag ctcacgtaga acaacacggc
     3121 cacggcggcg agcatcgtga acccttccag agcggagcgc cgcaccgggg gaacgttgag
     3181 cagcagctcg aagagcacgg cggtgatgcc actcgcggcc accgcggccc atgcgcccag
     3241 ggcgacctcg cggcgccggg cgggtgcacc ggccttggtg aggaagctca tcaacgcggc
     3301 gatgatgagg atcgcctcga agccttcgcg caccagcagc atcaacgatt gcaggaacag
     3361 gttggcgccg gaggcccggt ccacgaggag gcgctcggcg tgctccaggt cgcccagcag
     3421 cgctcgctgg aggacccgca actcggcggg aggcgcaccg gcggccgccc ggctccgtaa
     3481 ccgggcgaac tcaccctcca gtcgcgtcgc cagcccgggg tcgtgcgccc gcacgtcggt
     3541 ctcgacgccc tcgaacgtca ggtatgcgtc gaacgccagc cggtccgagc cctgggagac
     3601 ggcggaatcg agctgccgtc gtaccaccgc catgatcgcg gcggcctgtg agccgccgta
     3661 ttgcagggtc agggcgtacg cggcgaccgc ccagcggtcg tcctcggaca gcgtgtgttc
     3721 gaacgtcggc atcgccgtgc ccggcacacc gagcagcagc tgccggtaga ggtcgacgcg
     3781 cgacttggcg cccatcaccc ggggatcggc gagattcgca ggcggtggcg tgagtccccg
     3841 ggcccccggc ccgtcgcccc gcccggtcgc gccgtgacag gaggcgcagt gctccacgta
     3901 gagctccgcg ccgcgcgcca gcgatggcgc gaccaccgga accgggaaca ggtcgccgct
     3961 cgaacgggcc aggcgcccgg ccagagcctc ggccaagccg ctgacctgtg ccggcgcggc
     4021 ggtgcggtcg agcagggcgg ccatcgcgcc cagggtactg tcggcatagg cccgtgtatc
     4081 ggcggggagc acgtgaacgt cgaggcgggc ctgctggatg aagagccccg cctccttgac
     4141 ctcttctgca tcggtgacgc gcccgccctg caccgggacg ccgagcgcgt actccttggc
     4201 ggccagctga gcggccgcga cgatgcgccg cgcgacggac acggaatcca tgacctgagc
     4261 gggcagacgt acgctggccg cggccagaac ggtcaggatg agaatgggct tgcggtagat
     4321 gagaatggtt ctcagaaagg gtgtgccgaa aactagaaag ggcctttcac gaaagctaga
     4381 ccgcctccac aaccgccgtc gcgaccgccg ccgcgtgcgt gtgcgacagc gaaacgaaga
     4441 tacgcgagac cccgagctcg ctcgcccgct gtgccgcgag gcccgtcagc acgacgtcgg
     4501 gccggccgtc ctcgtcccgg accacctcga tctcccgcca cccgatgccg cgagcggccg
     4561 ggctcccctg cagcgccttg tacaccgcct ccttggtggc cagccgcgca gccacgctct
     4621 cggcggggtc cgtcttggaa cggcaatagg ccgcctcacc cggcgtgagc agccgatcgt
     4681 agacgtgcgt tcccttctcg gcgagcatgc gccgcacccg ctccacttcg acgagatcga
     4741 cgcccacccc gacgacgctc atcgggcgac gaacacgcgc cgccgcgaca cgcgcccgtc
     4801 cagctcgagc accacgagat acgcgccgtt ggcgatgcga tgcgagccgg cggtgaggtc
     4861 ccaggcatac tcgatcgtgc ccgcgggcaa cgtcgtagag atcaaccgtt cgcccgtgaa
     4921 cgtgtagatg cccaagcgcg gcgctcccgc gccggcgggc caggagatcc acacctggtt
     4981 cccgcgcacc gggttctccg acagtgcgag cgccgcaggt ggagccgggc tcaggaacgc
     5041 ggcggtggaa tcactcgcaa accccacggc atccacccac caggtgaagc ccacggtgat
     5101 gaaccgcacg cgggcgccgc gcgcccctgt gagcatcggc aggtccaggc gcatcggata
     5161 ccacgccggc ccgtcgccca ccacgtccgc cacggtggtc cacgtgctcc cgctgtcggc
     5221 cgacacctgc accagtccgt gctgttcggg cgtgtaggtc gagccgcgat gtctggtcca
     5281 gaactgcagc cagagcctcg tccgtccacc cagatcgacg accggagaga tgagcgtatc
     5341 cagcgctccg atgctcgtgg tccaggacca gctgcccgcg accgcgtccg tggagcgctg
     5401 ccagccgcgc caaccgaggt cgccgtcctc ggcgccggcg agcgagagca gttccgccgt
     5461 cggccctgca ccccgacccg catcatccac ccggagcgcc gccggacggt cgccgctcag
     5521 ggtcgcgtgc cacaccgtgc ggagcgtgcc gcgcgacagc gagtcggcgg tcagcgaccg
     5581 ctggacgatg atgcccccgg cgccccgcac cgacagggcg aacggctgcg gatcggacga
     5641 cgagagactc acccgcgctt ccggccagat cgtctcgatg cggggcggcg acgcgaccgc
     5701 gccggtcgga ccggcggcca acgcgggatc tcccgccgcc gcgatgaccg ccagcgcgaa
     5761 gggcaggttg tcgcggaaca cgcgccgcac cagcgacgag tcgtcgggga acgcaaaccc
     5821 gtagtacaac cccgagtcgg gcgtgcagca gccgctcgtc agctccggcg tgaacgcgat
     5881 cgtgccgtgg gcgcgatacg cccagtcggt gtactcgccg ttcgtcgggt agagattcca
     5941 ccccggtccc gggtggtagt aggaatgaaa gctcgccggg acgctgtcgc gcaccgccgg
     6001 ggcgagatcg gttcccgcca gcgcccggaa cagcggcacg tcgggagcga acgcgcccgg
     6061 caggtaacca taggggtaga tcaccagtcc ggagtaggtg tggtaggaca ccgaaaccac
     6121 gggcggatgc gcggcgtgga acgcgatcac ggcgcgcgtc tcgggctcgg aggccggcgc
     6181 cggtccgcgg taggtctccg ctcccgtcag cccgctcgag cccgcctcgt cgtaggccca
     6241 gaacgccgga taattgcggt tcaggtccac gccgtaggac ccgtcgccgt tcaggcgccg
     6301 gttcttcctc cagtagcggt ccgtcgtgaa ggtgaactgg tagccgtcgg ggttctcgac
     6361 cgggatcacc cacacatccc gcgaggcggt caacgcggcc gccgaatcgg ccagccagcg
     6421 gatgagcgcc atcgcgacct cggtcgagac ccattcacgc gcgtgatgcg tgcccaggaa
     6481 gagcacgttg gggcgcccgg gggcgtcggc cgcgggtccg atcttcacgg cgagaatcgg
     6541 ccggccttcg acgctggcac cgatcgaatc gacatggatc agcgtatcgg ccgcggccca
     6601 ggcggcgagc gtggcccgga tgcccgtccc cggtctgtcg aaggagcgaa acaccgcgaa
     6661 actgtccccg gcaggaccgg cggcggggat ccccacaggt ggagcggcga ggaatcctcg
     6721 ggtcgccagc gcgcgctcgg tctcgggcga cgcgacgacc agggcctcga gtcctcccgc
     6781 caccacccgg gcccccgcga cctcgaaccc cagtcgcgcc agcgaatcga ggccagccgg
     6841 ccccgacacg cgcaccgcca gcggacgcag ctgcccggcc gcggacgccg cgggtgcggc
     6901 agccagggcg aggatcagaa tcccagcgcg ccggcgagcc atgcgggagc ggagaggtag
     6961 ccccccagca cgagggcgag ccagacgacg acggcggcca acggcagcca caccgcgaac
     7021 acgggcatga gggacggccg ccaggcacga atcgcgtcga cctccggcgc ccagcgggcc
     7081 cgctcctgat cgaccagcgt ctcgagggcg agccaggcgg cctcgagccg ccgcgccgcc
     7141 aactcctgcg tcgcccgcca cgccgcgatc atctccgcat cgcccggcca gtcacggtgc
     7201 aacgcctccg tcgcccgggc cacatcttcc agtgccgcct cgaagccctc ggcgcccgag
     7261 gcgagccggg cggcgatggc gcggcgctcg ccgggcgaca aggggagctg ccgggagcgc
     7321 cgggcgggca tacgtacgcg ccgcgcttcg agcgccagcg ccgcgtcgag tccctgggcc
     7381 acgcgttccg ccgcccggcg caccgcttcg ttccacgcct cgctccacgc gtcgcggtcg
     7441 agctggcgca acgcccgggc gggagcggca tccgcggcat cgcggcgggc cgacccggcg
     7501 cattccatca cccgcgtggc cagcgcctcg cgcacgtcgt ccaacgggac ccacggatcg
     7561 ctcagccggt ggggaacgat cacccgtccc aggggcggtc cgagctcggg aaccgggagc
     7621 gtcaccgcca tttccgctct catgcgaggc gcgccttgcg cccggtccgc cgccggtagg
     7681 cggtgacgaa atgcggcaac gactccgcga gaaaccgctc gcgcggcagt cccgtggcgg
     7741 cgacggcccg ctcgatcgcg gcgggcgggc cctccacctc gagcagcgtg tccatccgcg
     7801 ggtagcgttc caggcgcagc atggccgctc ccagccggta gacttcgatg cggcggtcga
     7861 tcttcatcga gacgcgatag cgcaggcggc cgagcagcag gtccatcgcg tccgggtcgg
     7921 ccacagcgat ctcgagctcg gcgcggagcc ggtatccgcc acgccggccg gggcgggtgc
     7981 tcgcggcgcc tttccaggcc agcacggcat ggcctgctcc gcgaccatcg gggcggtaca
     8041 cccgtagccg gaggacgtcg tcgcggtgcg cgagctcgcg cgcgtggtca tagcgccggt
     8101 cgctcatccg gccgcggaat tccagacggg cgccggcgcg gcgcagcgcg cgccgcagcg
     8161 cggcgacgtt cgtcacccgg gccttcacct cgagctcggc gttgggccgc ggaccgttgc
     8221 ccgtacggcg gggagcgctc acgcgagcag tgcctgcagc acggccgccg tgtcggacag
     8281 atcggcgaac acgtgggtgg cgccggccgc ggcgagcgac ccgacgtcgt aatgaccggt
     8341 ggccaccgcg accgtgcgcg cgccgatggg ccgggcgcag gcgacgtcgt ccggcgtgtc
     8401 gccgatgacg atcacgtcgg ccccggcgat cgtgcgtccc gtcagcgatt cggcgcgggt
     8461 ccgcgcgatc gcgggcagga ggggacgatc gggagcgtcc gacccatagg cgccgacggc
     8521 gaaccgcgcc gggtcgatgc ccgccgagcg cagcttcagg gccgccccgc gctcgaggtt
     8581 gccggtgagc aggccgacca gcgcggcgcc ggccgcttcc ggacccgcca gcgccgtcag
     8641 cagctcacgc acgccggcca tcacgcgcgt ctgcgagggc cgggtgagct cgtcctccag
     8701 cagctccaag taccgcgcgc acaccgcctg aatccgttcc tgcatgccgg tatcgccgtg
     8761 ccccgcgagc gagagcagct cccgcacgat ctgcggatcg gtcttgccgt cgaagcgatg
     8821 gtcgccgatc ggtccggccg tgccgaccag ctcgagcagg gcgcggtgga tcgcgcggcg
     8881 cccggcgccg tcggtcagca ggatggttcc gtcgatgtcg aacagcacga ggcgcatcag
     8941 gactccacgc gtcgcacgcc cacgacctga tcccgcaacc gctgcaggcg cggcacgtcg
     9001 gcgagaaagt tttcactcac cacgccgccg cacgacaggg tcgagtgcac gatgcggccg
     9061 gcgccggccc agagggcgac gtgggacacg cgcccccgct cggcgaagaa cagcaggttt
     9121 cccttctcgt acccctcacc ggccggggcg ggaggaatct cgctgcccac ggtgaattgc
     9181 tgatcgctgt cgcgcggcag ggccacgccg cgcgccgcgt a
//
LOCUS       DBNGZW010000651         2820 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_403038, whole genome shotgun sequence.
ACCESSION   DBNGZW010000651 DBNGZW010000000
VERSION     DBNGZW010000651.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_403038"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(39..1370)
                     /locus_tag="AC16JC_15315"
                     /pseudo
     CDS             complement(39..1370)
                     /locus_tag="AC16JC_15315"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
     gene            complement(1289..>2820)
                     /locus_tag="AC16JC_15320"
     CDS             complement(1289..>2820)
                     /locus_tag="AC16JC_15320"
                     /inference="COORDINATES: protein motif:HMM:NF016101.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR5166118.1"
                     /translation="FDLSPSTADVAAATGSDRLAGRIPVGWYPTALLVRGDKLLVANG
                     KGAGTGPNRWLPQPGRRRPPEAVDTGYTLGQIKGSLSIVPAARASGAELAALTQRVAH
                     ANRWDERRPAGPSYPPFEHVIYVLRENRTYDQVLGDLPQADGDTSLVLFGRGVTPNAH
                     ALAERFGIFDRFFVNAEVSADGHNWSMAAYASDYTQKTAPIHYSDSGGRSYDFQGENR
                     GQVPADDVAEPASGYLWDLAARAQIDFANFGEFTREERGEGADSVRWYRGLKAFLAAH
                     TDTTYPGWDLSIPDQRRADVWLSSFRRWVQAGRMPALQIVYLPQDHTSGGAAGAPSPR
                     AMVADNDLALGRLVDALSHSPFWKSTVVFVMEDDAQNGPDHVDSHRSPLLVISPYAQG
                     GVLHRFTNTTDVLRTMEEILHFDALSQFDHYGHALRDVWRDTPDTAPYTARTASVPLD
                     ERNPGRGRAARETRRLDLATADAADMDLLNRVLWLVMKGEGVPYPGTHRMSALEVRRS
                     R"
BASE COUNT          415 a          998 c         1007 g          400 t
ORIGIN      
        1 tcgatggccg aagctcgggt gttcctggag ctcgacgact agcgacgcag cagcgtcgcc
       61 gtcgaatcgg ggctgccgtc gttcgacgcc ggtgtgagtc cgaggatccc cgcgatcaga
      121 tcgtagacgt ggatgttgcg gaacgcgggc gccacgtagc cgctccggaa cgccggtccg
      181 gaggcgagga agatcgcccg catcaccgac acggtgtcgt cgtacccgtg ggccccacca
      241 cgcagcgtct ccgggtgttg gagcacatcc gaccgtcgca gggccaccga ccacccggga
      301 tcggccaggg cgaggatcgg cgggatacgg cggttgtcac ggtaatggaa tcgcgccggg
      361 gtcccggccc tgcgccacac ggcgagatgc ggatgcttgc cgtacagcgc gcggtacaca
      421 ctgtcctcca tccccggcct gggccagagg ccgaagagcg ggttcccgcc cacgctgcgg
      481 gcgagcaacg cgagcgacag ataatcgtcc agcacgacga cgctgtccgg cgtcgtcggc
      541 gacatgccgt ggtcggagac gacgatgacg tcgaccctgg agcgcacgcc gctgcgcgcg
      601 atgccgtcga gcagcatccc caccgcgctg tccacccgca agaccgccgc ggccacctcc
      661 gcgctgccgg ggccgctctc gtgtcccgca tggtccacgt cgctgaagta gagcgtgacg
      721 aacgccggcc gtcgctccgc cggcagggcg agccagtcga gcacctgccg cacccgcgcg
      781 gcgttcggca cccgcgtgtc gtagcgctcc cattagtgcg gccgcacggc cgcgatggcg
      841 gcctcgctgc ccggccagaa gaacgcggcc gcgatccggt gctgtttctc cgccgtcacc
      901 cacagcggct cgccccccca ccagcgggcg tcgctcaccg cggcgctgtc gctgatccga
      961 aacagcgcgt cgaacaccgg gtcgtagatc gtgttctcca ccacgccgtg atgctcgggg
     1021 aagaggccgg tgacgatcgt gtagtggttg ggaaacgtct tggtggggaa cgccggcacg
     1081 agcggcgccc gtacccccgc ggagatgatg cggtgcaggt tgggcgacgc gtaccgctcg
     1141 aggtagtcgt agcggaagcc gtccagcgag accagcacca cgatcggcgc ggcctcgggc
     1201 cccggcgccg cgcggccggt ggcccccggg ccggcgcagg cgacgaggag gaccccgagc
     1261 cctccgcgga gccggcccgc gaggccggtc accgcgagcg ccgcacctcg agggcggaca
     1321 tgcggtgcgt cccgggatac gggacgccct cccccttcat caccagccac agcacccggt
     1381 tgaggaggtc catgtcggcg gcgtcggccg tcgcgagatc gagccgccgc gtttcgcgcg
     1441 ccgcccggcc acggccggga ttccgctcgt cgagcgggac ggacgccgtg cgcgccgtgt
     1501 atggcgccgt gtccggggtg tcgcgccaga cgtcacgcag cgcgtgcccg tagtggtcga
     1561 actgggagag ggcatcgaag tgcaggatct cctccatcgt ccgcagcacg tcggtggtgt
     1621 tggtgaagcg atgcaggacg ccgccctggg cgtagggcga gatcacgagc aggggcgagc
     1681 ggtgcgaatc cacgtggtcg ggcccgttct gggcgtcgtc ctccatcacg aacacgaccg
     1741 tgctcttcca gaacgggctg tgggagaggg catccacgag ccggcccagg gcgagatcgt
     1801 tgtcggcgac catggcccgg ggactcggcg caccggcggc gccccccgac gtgtggtcct
     1861 gcggcagata gacgatctgc agcgccggca tgcggcccgc ctgcacccag cggcggaagc
     1921 tcgacagcca gacgtcggcg cgacgctggt cggggatcga gaggtcccac ccgggatagg
     1981 tggtgtcggt gtgcgccgcg aggaatgcct tgaggccgcg ataccaccgg accgagtcgg
     2041 ccccctctcc tcgctcctct ctcgtgaatt caccgaagtt ggcgaaatcg atctgcgcgc
     2101 gggcggcgag gtcccacagg taccccgacg ccggctccgc gacgtcgtcg gcgggcacct
     2161 gtccgcggtt ctccccctgg aagtcgtagc tgcgcccgcc gctgtccgag tagtggatcg
     2221 gcgccgtctt ctgcgtgtag tcggatgcgt acgcggccat cgaccagttg tgcccatccg
     2281 cgctgacctc ggcgttcacg aagaaccggt cgaagatccc gaagcgctcc gccagcgcgt
     2341 gagcgttcgg cgtcacccca cggccgaaca ggacgagcga ggtgtcgccg tcggcctgcg
     2401 gcaggtctcc cagcacctga tcgtaggtgc ggttttcgcg cagcacgtaa atgacgtgct
     2461 cgaacggcgg gtacgacgga cccgcgggac gccgctcgtc ccaccggttg gcgtgtgcca
     2521 cgcgctgcgt cagcgcggcg agctccgcgc cgctcgccct ggccgcgggc acgatcgaca
     2581 ggcttccctt gatttgtccg agcgtgtagc cggtatcgac cgcttcgggc ggccgccggc
     2641 ggcccggctg cggcaaccag cgattgggac ccgtgccggc cccctttccg ttggccacga
     2701 gcagcttgtc gccgcgaacc agcagcgcgg tgggatacca gcccacgggt atccggccgg
     2761 cgagccggtc ggaccccgtg gccgccgcga cgtccgcagt cgagggcgac aggtcgaaca
//
LOCUS       DBNGZW010000652         3832 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_672692, whole genome shotgun sequence.
ACCESSION   DBNGZW010000652 DBNGZW010000000
VERSION     DBNGZW010000652.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3832)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_672692"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            87..1094
                     /locus_tag="AC16JC_15325"
     CDS             87..1094
                     /locus_tag="AC16JC_15325"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HIR5166119.1"
                     /translation="MTSPAPAPPPDLLGKSLGPYHLERLLGQGGFAWVFLGRGPDGAP
                     VALKVLKPRYAGDPQFESRFRNESQTAAKLEHPNIIRIRAVGKDGRNVYFVMDYCPDT
                     LAARLGREGPLPEAAILPIARDVTRALGYAHTQGIIHRDIKPDNVLLREDGTAVLTDF
                     GIARAVSGYNSSTGHNMTIGTPQYLSPEQAQGRHLDHRLDFYSLGVTLYRAAIGELPF
                     NAGDWFELARMHVEDPPPSLRRKRPDISKRFERVVVKCLAKHPDDRYQNAAELEADLD
                     DVRDGARPTTDVGTAPLTSTEYQALRDVHTPTRWGQRITFILVGAALIAAIALLIRLM
                     R"
     gene            complement(1078..1482)
                     /locus_tag="AC16JC_15330"
     CDS             complement(1078..1482)
                     /locus_tag="AC16JC_15330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166120.1"
                     /translation="MASSSSSAIRTLLEEYVGGRVPADRLVPAVAEQYYRSGARAGRD
                     GLRPLMDVIERAAPGVVQLARVAEGPGFDIRLAERPFPAAYEAELRQAAQAVLESGAA
                     LAGGPEREAPATGGGFWSRFLGRVRRLFSAST"
     gene            complement(1454..2791)
                     /gene="rseP"
                     /locus_tag="AC16JC_15335"
     CDS             complement(1454..2791)
                     /gene="rseP"
                     /locus_tag="AC16JC_15335"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HIR5166121.1"
                     /translation="MLVTLVSFAVIVGVLIFVHELGHFVAAKSLGIQVHRFSLGFGKP
                     LLKVRRGETEYIIAALPLGGYVKMAGLEEEGMMGEVEGGKPAQPVDPARAFDRKPLWA
                     RIIVILAGVTMNVVLAYVLYVARASSAGIPVLATTEVDTVYPANLPPGAEALGALRPG
                     DRILRIDGDTLRGWGDFVDRILDGPATARFDVAGREPFTVRLGDGSPTARRALLRALP
                     PRIPPRLGLVLLGEAGYRAGLQQGDLIVRVNGDTVRSWTALVRTIRRSPGRPLEFMVW
                     RDDSLVPVRVVPAAHRDSGFGQIGADAEPPMRHERVALGRAMLDGWHYTWGGIGAVLG
                     GVGQLVLGRVSFRQSLAGPIEIASVSGQVARLGIDRFLDLLGFFSINLAVLNLLPIPV
                     LDGGQLVFLVAEGIRRRPLSLELRLRLTQVGFVFLLGLMSLAVVNGIFKFFGH"
     gene            complement(2782..>3832)
                     /gene="dxr"
                     /locus_tag="AC16JC_15340"
     CDS             complement(2782..>3832)
                     /gene="dxr"
                     /locus_tag="AC16JC_15340"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR5166122.1"
                     /translation="FRVVALTAHSNAELLHSQAKRWEPAYIGLVNGGTGDEGRGRGTA
                     CLVEAATHPDVRIVVNGIVGAAGLEATLAALRAGKRVALANKETLVMAGELVMHEARA
                     GGGEVVPVDSEHSAVLQCLTGREAQEVSRLILTASGGPFRTWDPTRITGATVEQALQH
                     PTWKMGRKITVDSATLVNKALEVIEAHFLFGLGYAQVEVVVHPQSVVHAFVEFVDGSV
                     LAQLGFPSMELPILYALTHPERVADQGQRRFDALTAGGGALTFEPVSAARFPAYALGR
                     AAGTAGGTAPAVFNAANEVAVQAFLDGRIAFGRIPEIIECVLADHAGGDAGSLQAVLA
                     ADARARRRALEVACS"
BASE COUNT          663 a         1430 c         1220 g          519 t
ORIGIN      
        1 tcttctcgtc catgcgtcga cattccctaa tcgttgtgag cacactaagt tagaccgcac
       61 atgcgcgaat gtaaaggcca ccgctcgtga cgtccccggc tcccgctccg ccgcccgacc
      121 tgctcgggaa gtcgctcggc ccctatcacc tggaacgcct gctcggccaa ggcggcttcg
      181 cctgggtgtt tctcggacgc gggcccgatg gcgcgcccgt cgccctcaaa gtcttgaagc
      241 cccgctacgc cggcgaccct cagttcgaaa gccgcttccg caacgagagt cagacggccg
      301 ccaagctgga gcaccccaac atcattcgca tccgcgcggt ggggaaggat gggcgcaacg
      361 tctacttcgt catggactac tgccccgaca cgctcgccgc ccggctcggc cgcgagggcc
      421 cgctgccgga ggccgccatc ctgcccatcg cgcgcgacgt cacccgcgcg ctcggctacg
      481 cgcacaccca gggcatcatc catcgcgaca tcaagcccga caacgtcctg ctgcgtgaag
      541 acggcacggc ggtcctcacc gacttcggca tcgcccgcgc cgtctcggga tacaactcct
      601 cgaccggcca caacatgacc atcgggacgc cccagtacct ctcgccggag caggcgcagg
      661 ggcgtcacct ggaccaccgg ctggacttct actcgctggg cgtcacgctg taccgcgccg
      721 ccatcgggga gctgccgttc aacgccggcg actggttcga gctggcgcgc atgcacgtgg
      781 aggatccgcc gcccagcctg cgccgcaaac ggcccgacat ttcgaaacgg ttcgagcgcg
      841 tggtggtgaa gtgtctcgcc aaacaccccg acgaccgtta tcagaacgcc gccgagctcg
      901 aagccgatct cgacgacgtg cgcgacggcg cgcggcccac caccgacgtc ggcaccgccc
      961 cgctgaccag caccgagtat caggccctgc gcgacgtgca cacccctacc cggtggggcc
     1021 agcgcatcac cttcattctg gtgggagccg cgctgatcgc ggcgatcgcg ctcctgatca
     1081 ggttgatgcg ctgaacaggc gtcgcacccg gcccaggaac cgcgaccaga acccgccgcc
     1141 cgttgcgggc gcctcgcgct ccggcccgcc cgccaacgca gctccagatt cgagcaccgc
     1201 ctgtgccgcc tgacgcagct ccgcttcata cgccgccgga aacggacgct cggccaatcg
     1261 gatgtcgaac ccgggtccct cggcgacgcg cgccagttgc acgacgccgg gcgcggcgcg
     1321 ctcgatgacg tccatcagcg ggcggagccc gtcccggccc gcgcgcgcac ccgaccggta
     1381 gtactgctcg gcgaccgccg gcaccagccg gtcggcgggg acgcgcccgc cgacgtactc
     1441 ctcgagcagc gtcctaatgg ccgaagaact tgaagatgcc attgaccacc gccaggctca
     1501 tcagcccgag gaggaagacg aaaccgacct gcgtcagccg cagccgcagc tcgagagaga
     1561 gtggacggcg acggatgcct tccgccacca ggaacacgag ctgacctccg tccagcaccg
     1621 ggatcggcag cagattcaag acggccaggt tgatcgagaa gaagccgagc agatcgagaa
     1681 agcgatcgat gccgagacgg gccacctgcc ccgacaccga agcgatttcg atcgggccgg
     1741 ccagcgactg gcgaaacgac acgcgaccga gcaccagctg ccccacccca cccagcactg
     1801 ccccaatccc cccccaggtg tagtgccagc cgtcgagcat cgcacgaccg agggcgacgc
     1861 gctcgtgacg catcggcggc tcggcgtcgg cgccaatctg tccgaacccg gagtcgcggt
     1921 gtgccgcagg aaccaccctc acggggacca gcgaatcgtc gcgccacacc atgaactcca
     1981 gggggcgtcc cggactgcgg cggattgtcc gcaccagcgc cgtccacgag cgcaccgtgt
     2041 cgccgttgac gcgcacgatg agatcgccct gttggagtcc cgcccggtag ccggcctccc
     2101 ccagcaggac aagcccgagg cgggggggaa tacgcggggg cagtgcgcgc agcaatgccc
     2161 gccgcgcggt gggcgagccg tcgcccaggc gcacggtaaa tggctctcgc cccgcgacgt
     2221 cgaatcgcgc cgtcgccggc ccatccagga ttcggtcgac gaaatctccc cagccacgga
     2281 gcgtatcgcc gtcgatgcgc aggatgcgat cgccgggccg tagagcaccg agcgcctcgg
     2341 ccccaggagg gaggttcgcc gggtatacgg tgtccacctc ggtcgtcgcc aagacgggaa
     2401 tacccgcgct ggatgcccgc gccacataca agacataggc gagcacgacg ttcatcgtca
     2461 cgcctgcgag gatgacgatg atgcgcgccc acagaggctt gcggtcgaag gcgcgggcag
     2521 gatccaccgg ctgggcgggc ttgccgcctt ccacctctcc catcatcccc tcttcctcca
     2581 gcccggccat cttcacgtag ccgccgagcg gcagcgccgc gatgatgtac tccgtctcgc
     2641 cgcgccgcac cttcaggagc ggcttgccga agccgaggga gaagcgatgc acctggatgc
     2701 cgagcgattt cgcggcgacg aagtgcccga gctcgtgcac gaagatgagg acgccgacga
     2761 tgacggcgaa ggacacgagc gttacgagca tgccacctcc agggcccggc gcctggcccg
     2821 ggcatccgcg gccagcaccg cctgcagcga tccggcgtcg ccgccggcgt ggtcggcgag
     2881 gacacattcg atgatttcag gaatacggcc gaaggcaata cgcccgtcga ggaacgcctg
     2941 cacggccacc tcgttggccg cattgaacac cgcgggcgcc gtgccgccgg ccgtgcccgc
     3001 cgcccgcccc agcgcgtacg cggggaaccg cgccgcggag accggctcga aggtgagcgc
     3061 gcccccgccc gccgtcagcg cgtcgaagcg gcgctgcccc tgatcggcga cccgctcggg
     3121 gtgggtgagg gcgtagagaa tcgggagctc catgctggga aaaccgagct gggcgagcac
     3181 cgagccgtcc acgaactcga cgaaggcatg cacgaccgac tgcggatgca cgaccacctc
     3241 tacctgcgca taccccaatc cgaagagaaa atgcgcctcg atcacctcga gggccttgtt
     3301 gacgagcgtg gcggaatcga ccgtgatctt gcgccccatc ttccaggtcg gatgttggag
     3361 ggcctgctcc accgtagcac cggtgatgcg ggtcggatcc cacgtgcgga acgggccgcc
     3421 ggacgcggtg agaatcagcc gcgacacttc ctgcgcctcg cgaccggtga gacactgcag
     3481 cacggcgctg tgctcggaat cgaccggcac gacttccccg ccgccggcgc gcgcctcgtg
     3541 catgacgagc tcgccggcca tcaccagcgt ttccttgttc gccagcgcga ctcgtttgcc
     3601 ggcgcgcagc gcggcgagcg tcgcctcgag ccccgccgcg cccacgatcc cgttcacgac
     3661 gatccgcacg tcgggatggg tggccgcctc gacgaggcat gcggtacccc tccctcgtcc
     3721 ctcatccccc gtccccccgt tgaccagccc gatataggct ggctcccagc gctttgcctg
     3781 ggaatgcagc agctcggcgt tcgagtgcgc cgtgagcgcg acgacgcgga ac
//
LOCUS       DBNGZW010000653         3174 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_607254, whole genome shotgun sequence.
ACCESSION   DBNGZW010000653 DBNGZW010000000
VERSION     DBNGZW010000653.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_607254"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1166
                     /locus_tag="AC16JC_15345"
     CDS             48..1166
                     /locus_tag="AC16JC_15345"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5166123.1"
                     /translation="MPGEPFLSSDEYDDRAHQLYNEGQYDEAIEVLREGLTLYPAAVE
                     LHVGMGYARLARDEYVWARRSFEEALTLDPDHEDSLAGLGEVLLKFGERPGAVACFDR
                     ILGLGFRDDHDLMLQVGRALFREGVLDQARRFFEVALNAHGDSAEAAACVGYAAHRLG
                     DESGSLFWLRRALELDPAQAEARIYLANLLYDRGEYEAALFHLERTEPDDHVDTLAIW
                     RLLELKRSVYKLPDGDPELVPWNARLGELTADATADDLLLAEIEAIGSGGQVRDPRQL
                     ELFGTLLTELQGMRKGAAGDIHRVGTRAGRIYVGTWEEIVRQMKDDAVEWTGGSVEDY
                     MAASARLGRQETGVTIPATDAESFLRGSAEAGLLRILL"
     gene            complement(1163..1546)
                     /locus_tag="AC16JC_15350"
     CDS             complement(1163..1546)
                     /locus_tag="AC16JC_15350"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR5166124.1"
                     /translation="MNAFSSYAALLLRLAVGGVFLRHGIMKVEGGIPALAGFLHGLGF
                     PFANVWAVVLMVVETVGAACVLLGVLTRVWAVCFVVEMIVAILRVQIPGGHNFELEAL
                     LLAGAASLVALGDGPIALGVKLKRG"
     gene            complement(1543..1905)
                     /locus_tag="AC16JC_15355"
     CDS             complement(1543..1905)
                     /locus_tag="AC16JC_15355"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: protein motif:HMM:NF014705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004014 - adenosylmethionine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine decarboxylase"
                     /protein_id="HIR5166125.1"
                     /translation="MSCEHLFVDMSGVIPSRLHDPQDLGALALATANAAGLHPAAPPI
                     VQSSPRGTSAVLACHGGHVAIHALPDEGLCYADIASVGAALPQRALDVIVRRLAPRDV
                     RTEALRPREHAPAHQERP"
     gene            complement(1902..3002)
                     /locus_tag="AC16JC_15360"
     CDS             complement(1902..3002)
                     /locus_tag="AC16JC_15360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166126.1"
                     /translation="MKPIAFVLALALPLASACSGDSGPTAILSLTPVLDSVFVGDTVP
                     APTVHYLDLNGNPLDPGTVRWLANDPNILQVDSLTGTAVALKPGLAVVQARARNLTAS
                     ALVVVSRPVSIALLLDTVYLMPGDTITVPVAVAHQAAGTPSVWFRTTPNAVFVLDSAN
                     GRDSAKAPGGPLPFIAFAALGADTAADSGTVEVVSLIDTTGGKAAYTLFGTLVRSTKS
                     AVQAINYPRAGDTATFRLRAFISQGGTAVEAVVITARTEVTGVGAYPIDSISPAEITG
                     TGSADPFCHPPRNWGSWSTILSTFRLQALSRPDGQITITKVVPVPNGLAIGGRFLFTA
                     RRTDIYSDPLGTVPVRGTFVAPLVAATSRCTP"
BASE COUNT          538 a         1020 c         1172 g          444 t
ORIGIN      
        1 accgccgttt cgtatcatag cgcaaccttc cagggggcag cgctcgaatg cctggggaac
       61 cgttcctcag ctccgacgaa tacgacgacc gtgcccatca gctgtacaac gaaggacaat
      121 acgacgaagc gatcgaggtg ctccgggagg gtctgaccct ctacccggcg gccgtagagt
      181 tgcacgtggg catgggctac gcccgcctgg ctcgtgacga atacgtgtgg gcgcgacggt
      241 ccttcgagga ggcgttgacc ctcgaccccg accacgagga ctcgctcgcc ggtctgggtg
      301 aggtgctcct gaagttcgga gaacggcccg gcgccgtcgc ctgcttcgat cgcatcctcg
      361 gcctcggatt ccgggacgac cacgacctga tgctgcaggt gggccgcgcc ctgttccgcg
      421 agggcgtgct cgaccaggcg cgccgtttct tcgaagtcgc cctgaacgca catggcgatt
      481 ccgccgaggc cgccgcgtgc gtcgggtacg cggcgcaccg gctgggcgac gagtcgggat
      541 cgctcttctg gctgcggcgc gcgctcgagc tcgatcccgc gcaggccgaa gcgcgcatct
      601 acctcgccaa cctgctgtac gatcgcgggg aatacgaggc ggccctgttc cacctcgagc
      661 ggaccgagcc ggacgaccac gtcgacacgc tggccatctg gcgcctgctc gagctcaagc
      721 ggagcgtcta caagctgccc gacggtgacc cggagctggt gccttggaac gcccggctcg
      781 gagagctcac tgccgacgcc acggccgacg acctgctgct ggcggagatc gaggccatcg
      841 gatcgggtgg ccaggtgcgc gacccgcgac agctcgagct gttcggcacg ctgctcaccg
      901 aactgcaggg gatgcgcaag ggcgcggccg gcgacatcca ccgggtgggg acacgggcgg
      961 gccgcatcta cgtcggcacc tgggaggaga tcgtccgtca gatgaaggac gatgcggtgg
     1021 agtggactgg cggatcggtg gaggactaca tggcggcgag cgcccgtctg gggcggcaag
     1081 agaccggcgt gacgattccc gcgaccgacg ccgagagctt cctgcgcggc agcgccgaag
     1141 cgggattgct gcggatcctg ctctagccgc gcttcagctt cacccccagc gcaatggggc
     1201 cgtcgcccag ggccacgagg ctggccgcac cggccaacag cagagcctcg agctcgaagt
     1261 tgtgcccgcc gggaatctgg acccgcagga tcgccacgat catttccacg acgaagcaga
     1321 cggcccagac gcgggtgagc accccgagca ggacacaggc ggcgcccacc gtttccacga
     1381 ccatcagcac gacggcccac acgttggcga acgggaaacc gaggccgtgc agaaagcccg
     1441 cgagcgccgg gatcccgccc tcgaccttca tgatgccgtg ccgcaggaag acccccccga
     1501 cggccaggcg aagcagcagg gcagcgtagc tcgagaacgc gttcatggcc gttcctggtg
     1561 cgcgggagcg tgctcacgcg gacgcagcgc ttcggttctc acgtcacgag gcgcgagccg
     1621 gcggacgatg acgtcgagcg cccgctgcgg cagcgccgcc ccgaccgagg cgatgtccgc
     1681 ataacacagc ccctcgtcgg gcagtgcgtg gatggcgaca tgtccgccgt gacaggcgag
     1741 aaccgccgac gtgccgcgcg gactcgactg gacgatcggc ggggcggcag ggtgcagtcc
     1801 ggcggcgttg gcggtcgcca gggcgagggc gcccaggtcc tgcgggtcgt ggaggcggct
     1861 gggaatcacg ccgctcatgt cgacgaacag atgctcgcag ctcacggcgt gcagcggctc
     1921 gtggccgcga ccaggggggc gacgaaggta ccgcggaccg gcacagttcc gagcggatcg
     1981 gaatagatgt cggtgcgccg ggcggtgaag aggaagcggc cgccgatcgc caacccgttg
     2041 gggaccggaa cgactttcgt gatcgtgatc tgaccgtccg gccgcgacag ggcctggagc
     2101 ctgaatgtcg agaggatggt ggaccagctc ccccagttac ggggcggatg gcagaacggg
     2161 tcggcgctgc ccgtcccggt gatctcggcg ggcgaaatgg aatcgatggg gtatgccccg
     2221 accccggtga cctcggtgcg tgccgtaatc acgacggcct caaccgctgt gccgccttgt
     2281 gagatgaacg cgcgcagacg gaaggtggcg gtgtcgcccg cgcgcgggta gttgatcgcc
     2341 tgcacggcgc tcttggtgct gcggaccagg gtgccgaaca acgtgtacgc ggccttgccg
     2401 cccgtggtgt cgatgaggct cacgacttcc accgtgcccg agtcggcggc cgtgtcggcg
     2461 ccgagcgccg cgaacgcgat gaaggggagc ggtccccccg gcgccttggc gctgtcgcgc
     2521 ccgttggccg aatcgaggac gaatacggca ttgggcgtgg tgcggaacca gacggaagga
     2581 gtgccggccg cctggtgggc gacggcgacg ggcaccgtga tcgtgtcgcc gggcatcagg
     2641 tacacggtgt cgagcagcag ggcgatcgac accggccgcg acaccacgac gagcgcggac
     2701 gcggtgaggt tgcgcgcgcg ggcctgcacg acggcgaggc cgggcttgag tgcgaccgcg
     2761 gtcccggtca ggctgtcgac ctgcaggatg ttcgggtcgt tggcgagcca gcgtaccgtg
     2821 ccggggtcga gcgggttgcc gttgaggtcg aggtaatgga ccgtcggcgc cggcacggta
     2881 tcgccgacga agacggaatc gagcacgggg gtcaggctga ggatggccgt gggaccggaa
     2941 tcgccgctgc acgcgctggc gagcgggagc gccagggcga ggacgaatgc gatgggcttc
     3001 atcgaatgcg gcggcgacct gctcagaaca tcgacatggc cagaaacgac cgcaggttgt
     3061 cgttcgtgac ccggaggcgg gaggagagca ggcgcacgca cgaccacagc accttgtagg
     3121 cgatgtcgcg attgaagtcc agcaacagct cgaagtccga gcgggagatc gtga
//
LOCUS       DBNGZW010000654         4591 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_672778, whole genome shotgun sequence.
ACCESSION   DBNGZW010000654 DBNGZW010000000
VERSION     DBNGZW010000654.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_672778"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="AC16JC_15365"
     CDS             complement(<1..164)
                     /locus_tag="AC16JC_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013520828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5166127.1"
                     /translation="MQRGSRIESRGLVKAYKGRRVVNDVSIHLQQGEIVGLLGPNGAG
                     KTTTFYMFTGL"
     gene            complement(620..1987)
                     /locus_tag="AC16JC_15370"
     CDS             complement(620..1987)
                     /locus_tag="AC16JC_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166128.1"
                     /translation="MILTLALLTLQQPAASPDTARGRPCVVVVDSIGHNYREVAGADG
                     TKTYYAGGGVLAHCQGTTTTLSTDSLAHYAALGRMDLIGRVRIRDTTMSLDANFASYF
                     VRDERLEARTNVVAVNKSNGSVLRGPNLTYWRAAPGVRDTLEMFASQRPTIHYRGGGV
                     ADTTEPYVIVADRVRFKGSDRMWAGGRSTIDRSDFAAQGDSMMLDQTAGLGVLIGKPR
                     VQGKGAQNYTLTGTRIELVLQARDIRLVKALGNGKAVSTDWVLTADTIHLAIDRRKLQ
                     QALAWGGEGKNSSRPHAVSALQDIRADSLVLDVPDQILTEGRGFGKAFSASKRDSTVA
                     NVRAQCTGDNTDCLSSCLRIAVDCIAGDTLTARWGQEPDSTGIPRSRIRRIIARGSAR
                     ALTHMYGRDTTAGPTLNYSRGKTIDIVLKQARIDRVTVGGQADGVHLEPRPPVSDTTA
                     KKDST"
     gene            complement(1984..2616)
                     /gene="lptC"
                     /locus_tag="AC16JC_15375"
     CDS             complement(1984..2616)
                     /gene="lptC"
                     /locus_tag="AC16JC_15375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HIR5166129.1"
                     /translation="MRKASSRYVKQERRAQASSTYVVARRFCLTVLLDVFCLTSFCLT
                     SFACSSGVKPTATVTAADTADQVLVNMDHYVTGDGIVRARVRADTAYFYQNSQSAELR
                     NVHVTFYGADGIETSTMTAREGTYHWGTGDMEGRGNVVVIGSGDGRTLKTEVMRYNQR
                     SNEVSSDLPFVFDGPGRHMEGAGFTSDPNFRNVTASRPHGSGGGIVLPNR"
     gene            complement(2619..3593)
                     /locus_tag="AC16JC_15380"
     CDS             complement(2619..3593)
                     /locus_tag="AC16JC_15380"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HIR5166130.1"
                     /translation="MSPSAQPDLVERGRRVLVLEGDAIQRAARELGTPFARAVELLAG
                     ARGRLIVSGVGKSGLIARKIAATFTSTGTPATFLHPVDSLHGDLGIVGRDDVAILLSK
                     SGESGELFGLVEQLKRLGVPIIAVTGGRDSSLARAATVVLDAGDAEEACAETLAPTTS
                     TTVALALGDALAVTLLEVKGFRREDFAALHPGGALGRRLLLRVADVMLTSGLPTLAPE
                     RPMRECVVLLAEKRGTVAVVNGTGELAGVVTAGDLTRLMERTDRFLDLPVREVMTRNP
                     KTTTADTLASAAVELMERHGIMALPVVDGGRTVVGMVHLHDLMKAGAV"
     gene            complement(3562..4134)
                     /locus_tag="AC16JC_15385"
     CDS             complement(3562..4134)
                     /locus_tag="AC16JC_15385"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KdsC family phosphatase"
                     /protein_id="HIR5166131.1"
                     /translation="MLDATLARRVRLVGFDVDGVLTDGGIYLGRVADHAVELKRFDIQ
                     DGLAVNLLRAARIAVVLVSGRDSEATALRAREIGVDEVVQDDAAKKLPAFAAILARRA
                     IAFDECAFAGDDLADLPVLRRVALPLAVANAVAEVKAVATYVTSAPGGHGAVREIAEA
                     ILKARGDWDGLVRQYLEERGDVAQRATGSR"
     gene            complement(4127..>4591)
                     /locus_tag="AC16JC_15390"
     CDS             complement(4127..>4591)
                     /locus_tag="AC16JC_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HIR5166132.1"
                     /translation="AGKSGRPVNGKKGQWMAPEQMTGAVAKVRGGGALEVAVTERGTF
                     YGYGDLVVDMRSFARLRAACGTPVIFDATHSVQQPGQGSDGASGGLRQFIPPLLYAAA
                     AAGADGFFLETHPDPAHAPSDGPNMLSLEELPGVVAVALDLWQRVREVPARA"
BASE COUNT          652 a         1646 c         1601 g          692 t
ORIGIN      
        1 agcccggtga acatgtagaa cgtcgtggtc ttgcccgcgc cgttggggcc cagcaggccg
       61 acgatctcgc cctgctgcag gtggatgctc acatcgttga ccacgcgacg gcccttgtac
      121 gccttgacca gtccccggct ctcgatgcgg ctgccgcgct gcaccgcccc gtccgccggc
      181 gcgccctgcg tctccgcacg ctccagcgtg gcggcggcgg cggcgagcag gcgcgccgtc
      241 acgccggcgc ggtcgggagc gagcagcggc cacaggtcgg cgtgcaccga cacgccggca
      301 tcgtcgcgcc attcgccggg caggtccacg acctgctcca gctcgaggcg cggcagcacc
      361 gacgtccgga ggttgtcgcg ggcgtcgtcc accgaaaggc tgcccgactc ctccagcgag
      421 gtgccgcgca cccgggcgcg tacctgcagg aattcttccc ggtaggccac cgccagtgct
      481 ccgaagggcg cgacgccgcg ctcatcggcc gcctggagga tcgcggcggc tgccagcggc
      541 agtgaggagt cgcgtgccgc gagcagggcc tcgatccggg ccatggcggt gctggcgggc
      601 ggcatgggga acggcgggct cacgtggagt ccttcttggc ggtggtgtcg ctgaccggcg
      661 ggcgcggctc gaggtgcacg ccgtccgcct gtccgcccac ggtcacgcga tcgatgcggg
      721 cctgcttcag cacgatgtcg atggtcttgc cgcgcgagta gttcaacgtc ggaccggcgg
      781 tggtgtcgcg gccgtacatg tgcgtgaggg cacgggccga tccgcgcgcg atgatgcggc
      841 ggatgcggct tcgcgggatg ccggtcgagt ccggctcctg tccccagcgg gccgtgagcg
      901 tgtcgccggc gatgcagtcg accgcgatcc gcaggcagct ggacaggcag tcggtgttgt
      961 cgcccgtgca ctgggcgcgc acgttggcga cggtcgagtc gcgcttcgac gccgagaacg
     1021 ccttgccgaa cccgcgcccc tcggtgagga tctggtcggg cacatcgagc accagggagt
     1081 cggcccggat gtcctgcagc gccgagacgg catggggccg tgacgagttc ttcccctcgc
     1141 ctccccaggc cagcgcctgc tgcagcttgc ggcggtcgat ggccaggtga atcgtgtcgg
     1201 ccgtcagcac ccagtcggtg ctcacggcct tgccgttgcc caacgccttg acgaggcgaa
     1261 tatcccgggc ctgcaggacc agctcgatac gcgtgccggt cagggtgtag ttctgcgccc
     1321 ccttcccctg gacgcgcggt tttccgatga ggacgccgag gccggcggtc tggtcgagca
     1381 tcatggaatc gccctgggcc gcgaagtcgc tgcggtcgat ggtggacctc ccgcccgccc
     1441 acatgcggtc gctgcccttg aagcggaccc ggtcggcgac gatgacgtag ggctcggtcg
     1501 tgtcggcgac tccgccgccg cgatagtgga tcgtgggccg ctggctggcg aacatctcga
     1561 gcgtgtcgcg gacacccggg gcggcccgcc agtaggtgag gttcggaccg cgcaacaccg
     1621 agccgttgct cttgttcacg gccacgacgt tggtgcgcgc ctcgagccgc tcgtcacgga
     1681 cgaagtaaga cgcgaaattc gcgtccagcg acatggtcgt gtcgcggatg cggacgcggc
     1741 caatcagatc catccgtccg agcgccgcgt agtgggccag actgtcggtg gacagcgtcg
     1801 tggtggttcc ctggcaatgc gccaatacgc cgccgccggc atagtaggtc ttggtcccgt
     1861 cggcgcccgc cacctcgcga tagttgtgcc cgatggaatc gactacgacc acgcacggcc
     1921 tgcccctggc ggtgtcgggg gacgccgccg gctgctgcag ggtcaggagc gcgagggtca
     1981 ggatcatcgg ttggggagga cgatgccccc gccgcttccg tgcggccggc tcgcggtgac
     2041 gttgcggaag ttcgggtcgg aggtgaagcc ggcgccttcc atgtgacggc ccgggccgtc
     2101 gaagacgaac gggagatcgg aggacacttc attcgaccgc tggttgtacc gcatcacctc
     2161 ggtcttcagc gtgcggccgt cgcccgatcc gatgaccacc acgttgcccc gcccctccat
     2221 gtcgcccgtg ccccagtggt aggtcccctc gcgggccgtc atggtggacg tttcgatgcc
     2281 gtcggctccg tagaacgtga cgtgcacgtt gcgcagctcg gccgactgcg aattctgata
     2341 gaaatacgcc gtgtcggcgc gcacgcgcgc ccgcacgatc ccgtcgcccg tcacgtaatg
     2401 gtccatgttc accagcacct gatcggcggt gtcggccgcg gtcacggtcg ccgtcggctt
     2461 tacgccggag ctgcaggcga acgacgtcaa gcagaaagac gtcaagcaaa agacgtcgag
     2521 cagaaccgtc aggcaaaatc gtcgagcgac gacgtaggtg ctcgacgcct gtgctcgacg
     2581 ctcttgcttg acgtatctgc tcgacgcctt tcgcattctc agaccgctcc cgctttcatc
     2641 aagtcgtgca agtggaccat gccgaccacc gtgcgcccgc cgtccaccac ggggagcgcc
     2701 atgatgccgt ggcgctccat cagctcgacc gcggccgagg cgagcgtgtc ggcggtggtc
     2761 gtcttcgggt tgcgggtcat cacctcgcgc accggcaggt cgagaaaccg gtcggtgcgc
     2821 tccatcagtc gcgtgagatc gcccgccgtg acgacacccg cgagctcacc cgtcccgttc
     2881 accaccgcca ccgtgccccg tttctccgcg agcagcacca cgcactcgcg catcgggcgc
     2941 tcgggtgcga gcgtcgggag ccccgacgtc agcatgacgt cggcgacgcg cagcagcagg
     3001 cggcggccca gggccccgcc cgggtggagc gcggcgaaat cctcgcgacg gaaccccttg
     3061 acctcgagta gggtgacggc gagggcgtcg cccagagcca gcgccaccgt ggtgctcgtg
     3121 gtgggtgcca gcgtttccgc acaggcttcc tcggcgtcgc ccgcgtcgag cacgaccgtc
     3181 gcggcccggg cgagcgagga atcgcgcccg ccggtcacgg cgatgatggg gacgccgagg
     3241 cgtttcagct gctccaccag gccgaacagc tcccccgact ccccgctctt cgagaggagg
     3301 atcgccacgt cgtcgcgtcc cacgatgccg aggtctccgt gcagcgagtc caccgggtgc
     3361 aggaacgtcg ccggggtgcc cgtggacgtg aacgtcgccg cgatcttgcg ggcgatgagc
     3421 cccgacttgc cgacgccgga gacgatcagg cgcccccggg ccccggcgag cagctcgacc
     3481 gcgcgggcga acggcgtccc cagctcgcgg gcggcgcgct ggatggcgtc gccctcgagc
     3541 accaggacgc ggcgcccgcg ctcaacgaga tccggttgcg cgctgggcga catcgccccg
     3601 ctcctccagg tattgccgga ccagtccgtc ccagtcgccg cgggccttga ggatcgcctc
     3661 ggcgatctcg cgcaccgcgc catggccgcc gggcgcgctg gtgacgtagg tggcgaccgc
     3721 cttgacttcc gcgacggcgt tggccaccgc gagcggcagc gcgacccgac gcagcacggg
     3781 caggtcggcg agatcgtcgc cggcgaaggc gcactcatcg aacgcgatgg cccggcgcgc
     3841 gaggatcgcc gcgaacgccg ggagcttctt cgccgcgtcg tcctggacca cctcgtccac
     3901 cccgatctca cgcgcgcgca acgcggtcgc ctccgagtcg cggccgctca ccagcacgac
     3961 cgcgatcctg gccgcgcgca gcaggttcac cgcgagcccg tcctggatgt cgaaccgctt
     4021 cagctcgacc gcatgatcag cgacccgccc gaggtagatc ccgccgtcgg tgaggacgcc
     4081 gtccacgtcg aagccgacga gacgcacccg gcgcgccagg gtcgcgtcaa gcacgggccg
     4141 ggacctcccg gacgcgctgc cagaggtcca gcgcgaccgc gaccacgccc ggcagctcct
     4201 cgagcgacag catgttcggt ccgtcgcttg gggcgtgtgc gggatcggga tgggtctcga
     4261 gaaagaaccc gtccgccccg gcggcggcgg ccgcatagag caacggcgga atgaactgac
     4321 gcagtccgcc gctcgcgccg tcgctgccct gccccggctg ctgtaccgag tgggtggcgt
     4381 cgaagatgac cggcgtgccg caggcggcgc gcagccgggc gaagctgcgc atgtccacca
     4441 cgagatcgcc atacccgtag aacgtaccgc gctcggtaac ggccacctcc agcgcgccgc
     4501 cgccgcggac cttcgccacg gcgcccgtca tctgctcggg ggccatccat tggcccttct
     4561 tcccgttcac cggccgcccc gacttccccg c
//
LOCUS       DBNGZW010000655         4863 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1154177, whole genome shotgun sequence.
ACCESSION   DBNGZW010000655 DBNGZW010000000
VERSION     DBNGZW010000655.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4863)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4863)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4863
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1154177"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="AC16JC_15395"
     CDS             complement(<1..402)
                     /locus_tag="AC16JC_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HIR5166133.1"
                     /translation="MIAHLIAWSLRNRFLVSLGTVALALAGLWAVRTTPVDALPDLSD
                     VQVVIQTEFSEQAPQIVEDQVTYPIASELLKVPGTRAVRGFSFFGLSLVYVLFEDGTD
                     LYWARSRVLEYLNQVQGRLPATAKTSLGPDAT"
     gene            complement(399..1571)
                     /locus_tag="AC16JC_15400"
     CDS             complement(399..1571)
                     /locus_tag="AC16JC_15400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5166134.1"
                     /translation="MKRVVVAGLAGIMVAAGCRAKPAPVGMTAEEHARMTAGGDATDT
                     ASAGRQSVHLTADQARAIGVRFTVVTRGPLARTVRTVGQVVPAEPNLADITPKIEGFV
                     DRLFVDATGVAVRRGQPLLAIYSPMLVSAQQELLTAIHLAQSVDSTAPEAWRNAQDLV
                     AAARRRLAYWDISAEQIDRLEHTGEVTKNLTLVAPFDGVVMEKMVVAGQGVMPGMKLY
                     RLANLASVWVEGEVFEQDLGLIRVGAPVHVEIAAYPGRRFAGRVSFVWPMVEAQSRTG
                     RVRVVLANPQGLIKPGMYATLLFEATLGRDLLSLPAEAIVQTGERNLVFVLAGDGSLV
                     PREVTVGGRADGRIQILGGVAEGDRVVASANFLIDAESRLGTGVPMAGMPGMEHKP"
     gene            complement(1568..2833)
                     /locus_tag="AC16JC_15405"
     CDS             complement(1568..2833)
                     /locus_tag="AC16JC_15405"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR5166135.1"
                     /translation="MRSPTRTMARVALLLVALLPALAQAQGSATDTLTLAAATDMAMK
                     ANPMLGASRLAADAAAQRVGPAGTLPDPQLQFGLMNRMIRQFGSTMDPMTMNQVQLMQ
                     MLPWPGKLGSAHRAAVHTAAAARADAEDQALMLAAQVRMAYFEVGAADRSLDVMRRTQ
                     GLLRDFLQVSTTMYAVGDAVQQDVLRAQVEVARMGEEITRMEQERVAMAARLNALLGR
                     EAGVPIPAVELPEPGAELPAADTLIALALTHRPALRAGAERVSAADATLAGARRELYP
                     DLQVGVAYQARPAYDNMVSLMVGITLPIFAGSRQLPMRRQMAAMRDMSSAELLNLRNE
                     TIARIVETRARAVRDADLARLYRSSILPQARGAVQAALGSYRVGRVSFMQLVDNQMTV
                     NRYEIETIRLLADYQQAVGDLIALTGGEL"
     gene            complement(2833..3090)
                     /locus_tag="AC16JC_15410"
     CDS             complement(2833..3090)
                     /locus_tag="AC16JC_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YMGG-like glycine zipper-containing protein"
                     /protein_id="HIR5166136.1"
                     /translation="MKALLDIRALAIAGAIASAVITAVCFGLYAMLGRPDPWMDLFYG
                     SGPTLLGWIVGMGEGAAVGAIAGAILGAVYNRMVRPATQVR"
     gene            complement(3188..3544)
                     /locus_tag="AC16JC_15415"
     CDS             complement(3188..3544)
                     /locus_tag="AC16JC_15415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166137.1"
                     /translation="MTRPARSAAKRVIATLVAITVLWLAPSSAFGAMPSSHGCAMPLA
                     SGSVVSSVAHHTGAPCQHAEQLGCPSGMCAATVAVQQERAIAARVTPRAVRELPVSIV
                     GPSGPRSAPPTPPPNR"
     gene            3742..>4863
                     /locus_tag="AC16JC_15420"
     CDS             3742..>4863
                     /locus_tag="AC16JC_15420"
                     /inference="COORDINATES: protein motif:HMM:NF013799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HIR5166138.1"
                     /translation="MITVDQLRSDYLLRWRSQLSGGLAQLLTSGAVFSNAYQDQAITE
                     PAPGHSTVLSGRWPAHTGIITNTLGVGDSTAPLLEVRGPGASPRRFRGTEFYDWLVAA
                     QPASRALSVSRKDRGAILPVGTARQDVYWYQDGVFTTSRYYRDSLPAWVRAFNALRLP
                     FRAAGRVIGTLLPDSAYAEPDSVPWENGGHDLSFPHRLPTDSNRAAIAYMTTSLMDSL
                     TLAFALAGVEALQLGRRGATELLAVSLSATDYIGHSFGPDSREVHDQVLRLDRNLGWF
                     LSRLADRYGRDNLLVVLSADHGVTPFPEFSQAHGHPEARWVSVDTLIATVNQQLDLLT
                     ATATPRGWLEFDTGLLFVKDNGRLAALGIRIDSVLTGVAR"
BASE COUNT          765 a         1685 c         1678 g          735 t
ORIGIN      
        1 ggtggcatcc ggtccgagcg aagtcttcgc ggtggccggc aagcgcccct gcacctggtt
       61 caggtactcg agcacgcgcg agcgcgccca gtacagatcg gtgccgtcct cgaacagtac
      121 gtacacgagc gagaggccga aaaaggaaaa gccgcgcacc gcccgcgtgc cgggcacctt
      181 gagcagctcc gaggcgatcg ggtaggtgac ctggtcctcc acgatctgcg gcgcctgctc
      241 gctgaattcg gtctgaatga cgacctggac atcggagaga tcgggaagag cgtccaccgg
      301 agtggtccgg accgcccaca gccccgcgag cgcgagcgcg accgtgccga gggacacgag
      361 gaaccggttc cggagcgacc aggcgatcag gtgcgcgatc atggcttgtg ctccatcccg
      421 ggcatgccgg ccatggggac gccggtgccg aggcgcgatt ccgcgtcgat gagaaagttg
      481 gcggacgcca cgacccggtc gccctcggcg acgccgccca ggatctggat gcggccgtcg
      541 gcgcgccccc cgacggtcac ctcgcgcggc acgagggagc catcgccggc caatacgaac
      601 accaggttcc gctcgccggt ctggacgatc gcttccgccg ggaggctcaa gaggtcccgt
      661 cccagcgtgg cctcgaacag cagggtggcg tacatgccgg gtttgatgag tccctgcgga
      721 ttggcgagta ccacccgcac ccgcccggtg cgactctgtg cctcgaccat cggccatacg
      781 aagctcaccc gtcccgcgaa cctccggccc gggtaggctg ctatctccac gtggaccggc
      841 gccccgaccc ggatcaggcc gaggtcctgc tcgaatacct cgccctcgac ccagacgctc
      901 gcgagattgg ccaggcgata cagcttcatc cccggcatga cgccctgacc cgcgaccacc
      961 atcttctcca tcacgacgcc gtcgaacggc gcgaccaggg tcaggttctt ggttacctca
     1021 cccgtgtgct cgagacgatc gatctgctcg gcggagatat cccagtacgc caggcgccga
     1081 cgggcggcgg ccaccagatc ctgcgcgtta cgccacgcct cgggcgcggt ggaatcgacg
     1141 gactgggcga ggtgaatggc agtgagcagt tcctgctgcg ccgacacgag catcgggcta
     1201 tagatggcga gcagcggttg gccgcggcgt accgcgactc cggtcgcatc cacgaacagc
     1261 cggtccacga atccttcgat cttgggggtg atgtcggcca gattgggctc cgccggcacc
     1321 acctgtccca ctgtgcggac cgtgcgagcc agcggaccgc gagtgacgac ggtgaaccgg
     1381 acgccgatcg cccgggcctg gtcggcggtc agatgcaccg attgacgacc cgccgaagcg
     1441 gtgtccgtag cgtctccacc tgccgtcatg cgcgcgtgtt cttcggcggt catgcccaca
     1501 ggagccggct tggcgcggca ccccgcggcc accatgatgc cggccaggcc ggccacgacg
     1561 acacgcttca tagctctcct cccgtcaacg caatcaggtc gcccaccgcc tgctgatagt
     1621 cggcgagcag acggatggtt tcgatctcgt accggttcac ggtcatctga ttgtccacca
     1681 gctgcatgaa cgacacacga ccgacgcgat aactgccgag cgcggcctgg accgccccgc
     1741 gcgcctgcgg caggatgctg ctccgataga ggcgggcgag gtcggcatca cgcacggccc
     1801 gcgcccgcgt ctcgacgatg cgcgcgatcg tctcgttacg caggttcagc agctcggccg
     1861 acgacatgtc gcgcatcgcg gccatctggc gccgcatcgg cagctggcgg ctgccggcga
     1921 agatcggcag cgtgatgccc accatcaggc tgaccatgtt gtcgtatgcg ggccgagcct
     1981 gataggcgac gccgacctgc aggtcgggat agagctcgcg tcgcgcgccg gccagggtgg
     2041 cgtccgcggc cgaaacgcgc tcggcgccgg cgcggagcgc agggcgatgg gtgagggcga
     2101 gggctatgag cgtatcagcc gccggcagct ccgcgcccgg ctccggtaat tcgacggccg
     2161 ggatggggac gcccgcttcg cggccgagca gcgcgttgag tcgcgcggcc atggccaccc
     2221 gctcctgctc catgcgagtg atctcttcac ccatgcgcgc gacctccacc tgcgcgcgca
     2281 gcacgtcctg ctgcacggca tccccgacgg cgtacatcgt cgtactcacc tggaggaagt
     2341 cgcgcagcag accctgggtg cggcgcatca cgtcgagcga gcgatcggcc gcccccacct
     2401 cgaagtaggc catgcggacc tgggcggcga gcatgagggc ctggtcctcg gcgtccgccc
     2461 gcgctgcggc agcggtatgc acggccgcgc ggtgtgcgct cccgagcttc ccgggccacg
     2521 gcagcatttg catcagctgc acttggttca tcgtcatcgg gtccatcgtg gacccgaact
     2581 gccggatcat ccggttcatg agcccgaatt ggagctgagg atcgggcagt gttccggccg
     2641 gccccacgcg ctgcgcggcg gcgtccgcgg cgaggcgcga agctccgagc atcggattcg
     2701 ccttcatggc catgtccgtc gccgcggcca gggtcagcgt atccgtcgcg ctgccctggg
     2761 cctgtgccag cgcggggagc agggcgacga ggagcagcgc gactcgcgcc atcgttcttg
     2821 tgggagagcg catcagcgga cctgcgtggc tgggcgcacc atccgattgt agaccgcgcc
     2881 gagaatcgcc ccggcgatgg cccccacggc cgctccctca cccatgccga cgatccagcc
     2941 caagagggtg gggccggatc cgtagaacag atccatccac ggatcgggac ggcccaacat
     3001 ggcgtacaga ccgaagcaga cggcggtgat cacggcgctc gcgatcgcgc cggcaatggc
     3061 cagcgcccgg atgtccagta gcgctttcat ggatcctcct tgcctcccaa ccacgcggag
     3121 acaagacgac gttcaggggc tacagccagt ctgcagcccg cacgtggtcg ccgtcaggcg
     3181 acgaggatca gcggttggga ggaggtgtcg gtggagcgga gcgggggccg gacggaccga
     3241 cgatgctcac cggaagctcg cgcacggctc ggggcgtcac gcgggcggcg atggcccgct
     3301 cctgttgcac cgcgacggtc gcggcgcaca tccccgacgg gcagcccagc tgctcggcgt
     3361 gctggcacgg cgccccggtg tggtgcgcga cggacgagac gaccgagccg gacgcgaggg
     3421 gcatggcgca gccgtgggag gagggcatgg cgccgaacgc cgacgacggg gcgagccaca
     3481 acaccgtgat cgccaccagg gtggcgatta cgcgtttggc cgcgctgcgt gccggtctcg
     3541 tcaagcgatc tcctggtgcg tcgtgtccgg ccgaagactg ccgtgcctcg catgaagaaa
     3601 tacgcaagga cgggtgaaga gcgctagatt ggacgcccct gctacggagt gacacccatt
     3661 ttcgacgtga tcgttccact gttgcttggg ttcgcgggcc cggctcgtgc ggctgcaccc
     3721 ctgaagccga agttggtcgt gatgatcacc gtggaccagc tgcgcagcga ctacctgctg
     3781 cggtggcgct cccagttgag cggcgggctc gcgcaactgc tgacgtcggg agcggtcttc
     3841 tccaacgcct atcaggatca agccatcacg gaaccggcgc cggggcattc gacggtgctc
     3901 tcgggccgct ggccggccca taccggcatc atcacgaaca ccctgggcgt gggcgattca
     3961 acggcaccgc tgctggaggt gagggggccc ggcgcatcgc cgcggcggtt ccgcggcacc
     4021 gagttctacg actggctcgt cgccgcgcag ccggccagcc gggcgctctc ggtgtcgcgc
     4081 aaggatcggg gggccatcct cccggtcggc accgcccggc aggacgtgta ctggtaccag
     4141 gacggcgtgt tcacgacgag tcgctactac cgcgattcgc tgccggcgtg ggtgcgggcg
     4201 ttcaacgcgc tgcggctgcc gttccgcgcc gcggggcgag tgatcggcac gctgctgccg
     4261 gattcggcct atgcggagcc cgacagcgtt ccctgggaga acggcgggca cgacctgagc
     4321 ttcccccatc gcctgccgac cgacagcaac cgcgccgcca tcgcctacat gacgacgtcg
     4381 ctgatggatt cgctgaccct ggcgttcgcg ctcgcgggcg tggaggcgct ccagctggga
     4441 cggcgcggcg ccaccgagct gctcgcggtc agcctctcgg ccaccgatta catcggccac
     4501 agcttcggac ccgactcgcg cgaggtgcac gaccaggtgc tgcggctcga ccgcaacctg
     4561 gggtggttcc tgagccggct ggcggaccgc tacgggcgcg acaacctgct cgtcgtgctc
     4621 tcggcggatc atggcgtgac gccgttcccg gaattctccc aggctcacgg ccatcccgag
     4681 gcgcgatggg tgagcgtcga tacgctgatc gccacggtca atcagcagct cgacctcctg
     4741 accgcgaccg ccactccgcg gggctggctc gagttcgata cgggattgct gttcgtgaag
     4801 gacaacgggc ggctcgccgc gctgggcatc cgcatcgaca gcgtgctcac cggcgtggcg
     4861 cgt
//
LOCUS       DBNGZW010000656         2106 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_61369, whole genome shotgun sequence.
ACCESSION   DBNGZW010000656 DBNGZW010000000
VERSION     DBNGZW010000656.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2106)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_61369"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="AC16JC_15425"
     CDS             <1..252
                     /locus_tag="AC16JC_15425"
                     /inference="COORDINATES: protein motif:HMM:NF013781.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HIR5166139.1"
                     /translation="GIPNVPQPDYGALAEGQRRRAAEARARREGPAVARALAGLRAAA
                     ANPDARLMPLMLEAVRARATVGEISNVLRETWGEYRPTI"
     gene            327..>2106
                     /locus_tag="AC16JC_15430"
     CDS             327..>2106
                     /locus_tag="AC16JC_15430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014896.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIR5166140.1"
                     /translation="MRVRIRVLSLAIALFAAASLRAQQLQYPPAPKGDVTDDYFGHRV
                     ADPYRSLEDLDAPATKAWVAAENALTFGYLAQLSLRDSIRARITALWNYPKITIPARE
                     AGQLWFRRNTGLQRQSVLYRASATGSDPVVVLDPNALFPDGATAVAQWSVSPDGRTLA
                     YTTAAGGSDLQDIHLRDLAAGRDLPDVVLHVKFTGVSWTQDSRGFYFSRFRSSAEQAS
                     LRNANTHHQLWYHTLGRSADRMVFERADDSTAFVGGGTSDDGRWLFVNSASGTSNNRL
                     WVADLKDPRHPNLAAPLQPVTTAEDADYNALGVVRDTLYLFTNWLAPRGRIVAAAVGD
                     SDRTHWRTIVPEGPDVLSSTLLVGERLVLGYLVDVQSRIRLYDLAGAPRGEVSLPDVG
                     SAGGLSGRTDGTDFFFQFASYLRPPTVYRYDLATGKLQPFMPVTTPFDPSPFETRATF
                     YQSKDGTRVPIFITARKGIAKDGRHPTLLYGYGGFDINLTPSFSPAVAAWLEMGGVYA
                     VANLRGGGEYGEAWHHSGMLEKKQTVFDDFIAAAEFLVSEGYATPRTLAIQGGSNGGL
                     LVGAAMTQRPELYAVALPAVGVMDMLR"
BASE COUNT          304 a          754 c          711 g          337 t
ORIGIN      
        1 ggcatcccga atgtgcccca gcccgattat ggcgcgctgg cggagggcca gcgacgccgg
       61 gccgcggaag cgcgggcgcg acgggagggg ccggccgttg cgcgcgcgct cgcgggactc
      121 cgcgctgccg ccgcgaatcc cgatgcccgt ctcatgccgc tcatgctcga ggcggttcgc
      181 gcacgggcca ccgtcgggga gatcagcaac gtcctgcgcg agacgtgggg cgagtaccgt
      241 ccgacgatct gactcgagcg gttgccgtca ccccggatag cggcgagact tctacccgtc
      301 ccctcgcaca cctccggagc cgtaccatgc gcgtccgcat cagagtcctt tcgctcgcca
      361 tcgcgttgtt cgccgccgcc tcgctgcggg cccagcagct gcagtacccg ccggccccca
      421 aaggcgacgt gaccgacgac tacttcggcc accgcgtcgc cgacccttat cgctcgctcg
      481 aggacctcga cgccccggct accaaggcct gggtcgcggc ggagaatgcg ctcacgttcg
      541 ggtacctcgc gcagctgtcg ctgcgcgatt cgatccgcgc ccggatcacg gccctgtgga
      601 actacccgaa gattaccatc cccgcgcgcg aggccgggca gctctggttc cgccgcaaca
      661 cgggactgca gcggcagtcg gtgctctatc gggccagtgc gaccggcagt gaccccgttg
      721 tggtgctcga tcccaacgcg ctgtttccgg atggagcgac ggcagtcgcg cagtggtccg
      781 tctcgcccga tggtcgcacc ctggcctaca cgaccgcggc cggcggctcc gatctgcagg
      841 acatccacct gcgcgacctc gcggccggca gagacctgcc cgacgtcgtg ttgcacgtga
      901 agttcaccgg ggtttcctgg acccaggaca gccgcggctt ctatttctct cgcttccgga
      961 gctccgccga gcaggcgagc ctgcgcaacg ccaacaccca ccaccagctc tggtatcaca
     1021 cgctgggcag gtccgccgac cggatggtct tcgagcgggc ggacgactcg accgccttcg
     1081 tgggtggtgg gacctccgac gatgggcgct ggctgttcgt gaactccgcc agcggcacgt
     1141 cgaacaaccg cctctgggtc gccgacctca aggatccgcg gcatcccaac ctggcggcgc
     1201 cgttgcagcc ggtgaccacc gccgaggacg ccgactacaa cgcgctgggc gtagtgcggg
     1261 ataccctcta cctgttcacc aactggctgg cgccgcgcgg ccgcatcgtc gcggcggcgg
     1321 tgggggacag cgaccggacc cactggcgca cgatcgttcc cgaggggccc gacgtgctgt
     1381 cgtccaccct gctcgtgggc gagcggctgg tgctcggcta cctcgtggac gtgcagagcc
     1441 ggatccggtt gtacgatctc gccggcgcgc cccgcggcga ggtctcgctg cccgacgtgg
     1501 ggagcgcagg cggcctgtcg ggccgcaccg acgggaccga cttcttcttc cagttcgcct
     1561 cgtacctgcg gccgccgacc gtgtaccgct acgatctggc gacggggaag ctgcagccgt
     1621 tcatgccggt gacgacgccg ttcgatccgt caccgttcga gacccgcgcc accttctatc
     1681 aatccaagga tggaacgcgc gtgccgatct tcattacggc gcgcaaaggg atcgccaagg
     1741 acggccgtca ccccacgctg ctgtacgggt acggcggctt cgacatcaac ctgacgccgt
     1801 ccttctctcc cgccgtggcc gcatggctcg agatgggcgg ggtgtacgcg gtggccaacc
     1861 tgcgtggtgg gggagagtac ggtgaggcct ggcaccattc cgggatgctc gaaaagaagc
     1921 agaccgtgtt cgacgacttt atcgcggccg cggagtttct cgtgagtgag ggctacgcca
     1981 cgccgcgcac gctcgcgatc caggggggct cgaacggcgg cctgctggtg ggggcggcca
     2041 tgacccagcg ccccgagctg tacgccgtgg cgctccccgc ggtcggcgtg atggacatgc
     2101 tgcggt
//
LOCUS       DBNGZW010000657         5168 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1562416, whole genome shotgun sequence.
ACCESSION   DBNGZW010000657 DBNGZW010000000
VERSION     DBNGZW010000657.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5168
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1562416"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..805)
                     /locus_tag="AC16JC_15435"
     CDS             complement(<1..805)
                     /locus_tag="AC16JC_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HIR5166141.1"
                     /translation="MATDTILRPGELKDILLKEIQAADLAAIDVREVGTVLEVKDGIA
                     RIYGLTSAMAGEMLEFTAAETGEKITGQALNLEEDNIGAVILGDYLQLREGDEVRRTG
                     RVLEVPVGKAMVGRVVDALGRPVDGLGPIQTSKTRKVEIVAPGIIKRQPVKEPLQTGI
                     KAIDSMIPIGRGQRELIIGDRGTGKTAIAVDTIINQKGQGVICVYIAIGQKNSTVAAV
                     VERLKEHGAMDYTIVVVASASEPAPLQYIAPYSGCTMAEYFMYDEKTATL"
     gene            complement(993..1760)
                     /locus_tag="AC16JC_15440"
     CDS             complement(993..1760)
                     /locus_tag="AC16JC_15440"
                     /inference="COORDINATES: protein motif:HMM:NF024242.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5166142.1"
                     /translation="MPVVPICHQRREARRALARGYPRRLGAVRACATPQAVLRLLAAR
                     LVDAVVVDVKSEPEIWLDLPGRYPRIPLFALSAFRPDDGRLLAQCHRAGFAGVLVEPV
                     DHAMAGEWVARRTAQQARRRALADAPRLLRLTEPLQLRTWEEVWRRVAEPTKTADVAR
                     ALRVTREHLSREFAAGGAPNLKRLIDLVRTACAADLLGNPGYTVGAVARILNYASPSH
                     LAGAARRVAGAVPQELRAMGPRGVLAAFLRGRTRSRL"
     gene            complement(1760..2239)
                     /locus_tag="AC16JC_15445"
     CDS             complement(1760..2239)
                     /locus_tag="AC16JC_15445"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5166143.1"
                     /translation="MAKRQRSQRETSAGGVVFRRDSDGLVRYLLIRDSYRNWGFPKGH
                     LEPGEPPADAARREVSEETGLTDLILHGPIRVIDWYFRFRGKTIHKYCHFFLFESRVG
                     DPVPQQDEGITQCAWHPQEEALTTISYDNARGVLRRAAEMVRALAAVSDGAGDGERA"
     gene            complement(2282..2932)
                     /locus_tag="AC16JC_15450"
     CDS             complement(2282..2932)
                     /locus_tag="AC16JC_15450"
                     /inference="COORDINATES: protein motif:HMM:NF024957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HIR5166144.1"
                     /translation="MRTISGILWLSGVMALVVPVTARGQGLRVGVKGGVNLATMGGSD
                     ASTFETACGCNITTRTAVVLGGFASFDLGRIVKLQPELYYIGKGTKIDVAGVAATIEV
                     SYLELPLLLVVAPRVQGTIHPSFFAGGELGVKVGCSLAASGASVSCSNASFDTKSLDY
                     GIVFGAGLGFPLGSGEALIDGRYDLGLSKFLDTNPSVSLSHRGIAILVGYSFRIGR"
     gene            complement(2992..3999)
                     /locus_tag="AC16JC_15455"
     CDS             complement(2992..3999)
                     /locus_tag="AC16JC_15455"
                     /inference="COORDINATES: protein motif:HMM:NF014523.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HIR5166145.1"
                     /translation="MKRENEFVVGVVVVVAFIAVVAGALWLSGAHIGRTEAIYTARFR
                     TVGGLSVGNPVVLRGVRVGRVEAIRLASGNWVEADMRIYAGVTLPQRPAVLATSVSLF
                     GEWAASLFSYDQPPDDPNVRQALAEAMALGGGRWPGATLPDIGQLTAQAGRIANDIAA
                     VAARVQTAFDSEAVSELRRSIRDFGQVADRLAKAANEQADNIGTVGTNLSKGSTALAR
                     AATVLESTLGRVDTATNQGQLATILNNASAASQDVRTASQNLRELMDATHRSQESFVR
                     VITEADTVLSRIVNRSGTLGLLVADSALYRETTLTMIQLRQLLSDVQANPRKYFTFSV
                     F"
     gene            complement(3996..4721)
                     /locus_tag="AC16JC_15460"
     CDS             complement(3996..4721)
                     /locus_tag="AC16JC_15460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013257036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5166146.1"
                     /translation="MIQFRDLHKGFDGHVVLDGFTLEVKDGETMVIIGLSGSGKSVAL
                     KHVVGLLQPDAGDVIVDGRPVSQLDREGLMALRREIGFVFQFAALFDSMTVGENVALG
                     LRRRGLSEEAIAQRVGEALELVDLVGTDDRLPAQLSGGMRKRVGIARAIALRPRYILY
                     DEPTTGLDPVTSAVMDRLMVRTRERLGVTGIVVTHDMRSAYTVGDRIAMLSEGRVRQV
                     GSVAEIQATADPVVRQFIEGRPE"
     gene            complement(4718..>5168)
                     /locus_tag="AC16JC_15465"
     CDS             complement(4718..>5168)
                     /locus_tag="AC16JC_15465"
                     /inference="COORDINATES: protein motif:HMM:NF014460.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MlaE family ABC transporter permease"
                     /protein_id="HIR5166147.1"
                     /translation="AGRVGASVAAELGTMKVPEQVDALETLAYDPLAYLVVPRLLAGT
                     VMVPAVVVLGIVTGIVTGWLTSMSLLDLSTPEFVKGLKFLYQFKDVWFALVKSASFGL
                     IITLVGCRLGLRTRGGAEGVGRSTTQAVVISAMSILVLDAFWAVVLL"
BASE COUNT          828 a         1871 c         1671 g          798 t
ORIGIN      
        1 acaacgtggc cgtcttctcg tcgtacatga agtactcggc catggtgcac cccgaatacg
       61 gcgcgatgta ctgcagcggg gcgggctccg aggccgaggc cacgaccacg atggtgtagt
      121 ccatcgcgcc gtgctccttc agacgctcca ccaccgccgc gaccgtcgag ttcttctgcc
      181 cgatcgcgat gtagacgcag atcaccccct ggcccttctg gttgatgatc gtgtccaccg
      241 cgatggcggt cttgcccgtg ccccggtcgc cgatgatcag ctcgcgctgg ccccggccga
      301 tcgggatcat cgagtcgatc gccttgatgc cggtctgcag cggctccttc accggctgcc
      361 gcttgatgat gccgggcgcg acgatctcga ccttgcgcgt cttgctcgtc tggatcggcc
      421 ccaggccgtc caccggccgg cccagcgcgt ccaccacccg gccgaccatc gccttgccga
      481 ccgggacctc cagcacccgc ccggtgcgcc ggacctcgtc gccctcacgc agctggaggt
      541 agtccccgag aatcacggcg ccgatgttgt cctcttcgag gttcagcgcc tgtcccgtga
      601 tcttctcgcc ggtctccgcc gccgtgaact ccagcatctc cccggccatc gccgaggtca
      661 gcccgtagat ccgggcgatg ccgtccttca cctcgaggac ggtcccgacc tcgcgcacgt
      721 cgatcgcggc gagatcggcg gcctggattt ccttgaggag gatgtccttt agctcgccgg
      781 ggcggaggat ggtgtcggtg gccatggggg ggttccaggt tcgtggttag cttgtgaaaa
      841 ttatcacata gtcactcccg ccgccacccc ctcaccaaaa ttgcggattg gcgattgcgg
      901 attgccgatt gcggaatcta aatggaactc agcccgcgtc gctggccgag aacccgcagt
      961 gcccaatccg caatccccaa tccgcaatga gattacagtc gcgacctggt tctccccctg
     1021 agaaaagccg ccagcacacc ccgcggcccc atggctcgca gctcctgggg gaccgcaccg
     1081 gcgaccctgc gggccgcacc ggccaggtga ctgggagacg cataattgag gatgcgcgcc
     1141 acggcgccca cggtatagcc cggattgccg agcaggtccg cggcgcaggc ggtccgcacc
     1201 agatcgatca gtcgcttgag attcggggcg ccgcccgcgg cgaattcgcg cgacaggtgc
     1261 tcgcgggtca cccgcagggc gcgcgccaca tcggccgtct tcgtcggctc ggccacgcgt
     1321 cgccacactt cctcccacgt gcggagctgc agcggctcgg tcagccgcag caggcggggc
     1381 gcgtcggcca acgcccgccg gcgcgcctgc tgcgccgtgc gccgtgccac ccattctccc
     1441 gccatcgcgt gatccaccgg ctcgaccagc acgccggcga accccgcacg atggcactgg
     1501 gcgagcaacc ggccgtcgtc cggccgaaac gccgagagcg cgaagagcgg gatccggggg
     1561 tagcgtcccg gcaagtcgag ccagatctcc ggctccgact tcacgtccac caccaccgcg
     1621 tcgaccagcc gcgccgcgag cagccgcagg accgcctgcg gcgtggcgca cgctcgcacc
     1681 gccccgagcc ggcgcggata cccgcgtgcg agggcccggc gtgcctcgcg ccgctggtga
     1741 caaatcggga cgaccggcat cacgcgcgct ccccgtcacc cgccccgtcg ctcacggcgg
     1801 ccagcgcccg gaccatctcg gcggcccgtc gcagcacacc gcgggcgttg tcgtaggaaa
     1861 tcgtggtgag cgcctcctcc tggggatgcc aggcgcactg cgtgattcct tcgtcctgct
     1921 gcggcaccgg atctccgacg cgggactcga agagaaagaa gtgacagtac ttgtggatgg
     1981 tcttgccgcg gaagcggaaa taccaatcga tcacgcggat cggaccgtgc aggatcaggt
     2041 ccgtcagccc ggtctcttcg ctgacctcac gccgggcggc gtcggccggc ggctcgcccg
     2101 gctccaggtg ccccttgggg aatccccagt tgcggtagga atcgcggatg aggaggtagc
     2161 ggaccaggcc gtcggagtcc cggcggaaca ccactccgcc ggcgctcgtc tcccgctggg
     2221 aacgttgtcg cttcgccatg cgctcgtccg ctctcccgct cggcccgggg ccgaccgccc
     2281 gctaccggcc gatccggaag gaatagccga ccaggatcgc gatgccgcga tgactgagcg
     2341 acacggaagg attggtgtcc aggaacttcg acaggcccag gtcgtagcgg ccatcgatca
     2401 gggcttcgcc gctccccagg ggaaacccca gcccggcgcc gaatacgatg ccgtagtcga
     2461 gggacttggt gtcgaaactc gcgttgctgc agctgaccga cgcgccgctg gcggcgagcg
     2521 agcatcccac cttcacgccg agctcgccgc cggcgaagaa gctgggatga atggttccct
     2581 gcacccgcgg cgccaccacg agtaacagcg gcagctcgag gtaggagacc tcgatcgttg
     2641 ccgcgacccc tgcaacgtcg atcttcgtgc ccttgccgat gtagtagagc tcgggctgca
     2701 gcttgacgat gcgacccagg tcgaacgacg cgaagccgcc gaggacgacc gcggtccggg
     2761 tcgtgatgtt gcagccgcaa gccgtctcga acgtgctggc gtcggatccg cccatggtgg
     2821 cgaggttgac gccgcccttc acgccgacgc gcaggccctg tccccttgcc gtcaccggga
     2881 ccaccagggc catgacgccg ctcaaccaca ggatgccgct gatcgtccgc atggagatgc
     2941 ctccggactg ctggtgatgg tcgtaggcgg gcacgggctc gcccgctgcc actagaatac
     3001 gctgaaggtg aagtacttgc gtggattggc ctgcacatcc gacagcagct gacgcagctg
     3061 gatcatcgtc agggtcgttt cccggtacag cgccgagtcg gccaccagca ggccgagggt
     3121 gccgctgcgg ttcacgatcc gcgacaggac cgtgtcggcc tcggtgatga cgcggacgaa
     3181 actctcctgg ctgcggtgcg tggcgtccat gagctcccgc aggttctgcg aagccgtgcg
     3241 gacgtcctga ctggcggcgg aggcgttgtt gaggatcgtg gccagctgac cctggttcgt
     3301 cgccgtgtcc acgcgtccca gcgtcgactc gagcaccgtg gccgcgcgcg cgagggccgt
     3361 ggatcccttc gagagattgg tgccgaccgt cccgatattg tcggcctgct cgttggcggc
     3421 cttggccaat cgatcggcga cttgcccgaa atccctgatc gaccggcgca gctcggagac
     3481 cgcttcggaa tcgaaggccg tctgtacccg ggccgcgaca gcggcgatgt cgttcgcgat
     3541 ccgccccgcc tgcgccgtca gctggccgat gtcggggagc gtggcgccgg gccagcgccc
     3601 gccgcccagg gccatggcct ccgccagggc ctggcgcacg ttgggatcgt cgggcggctg
     3661 gtcgtaggag aagagcgacg ccgcccattc cccgaacagc gacacggacg tcgcgagcac
     3721 cgccggccgc tggggcaacg tcaccccggc gtagatccgc atgtccgctt cgacccagtt
     3781 gccggatgcg agccggatcg cctcgacgcg cccgacccgt acaccgcgca gcaccaccgg
     3841 atttcccacg ctcagaccgc ccacggtccg gaaccgcgcg gtatagatcg cttccgtccg
     3901 gccgatgtgg gccccggaca gccacagggc gcccgcgacc accgcgatga acgccaccac
     3961 gacgaccacg ccgacgacga attcgttctc gcgtttcact cgggccgccc ctcgatgaac
     4021 tggcgtacca ccggatcggc ggtcgcctgg atctcggcaa cgctccccac ctgccggacg
     4081 cgtccctcgg acagcatcgc gatgcgatcg ccgaccgtat aggcgctgcg catgtcgtgg
     4141 gtgaccacga tgccggtcac tcccagacgc tcgcgcgtgc gcaccatgag ccggtccatc
     4201 accgccgagg tcacgggatc gagccccgtc gtgggctcgt cgtacaggat gtagcgcggc
     4261 cgcaaggcga tcgcccgcgc gatcccgacc cgcttgcgca tgccgccgga cagctgggcc
     4321 gggaggcgat cgtcggtgcc cacgaggtcc acgagctcca gcgcttctcc tacccgctgc
     4381 gcgatcgcct cctcggacag gccgcggcgt cgcaggccca gcgcgacgtt ttcgcccacg
     4441 gtcatggaat cgaacagcgc cgcgaactgg aacacgaatc cgatctcccg gcgcagcgcc
     4501 atgaggccct cgcgatcgag ctgcgacacc ggacgcccgt caacgatgac gtctccggca
     4561 tccggctgca acaggcccac cacgtgcttg agtgccacgc tcttgcccga cccggagagg
     4621 ccgatgatca ccatcgtctc gccgtccttg acctcgagcg tgaatccgtc gagcaccacg
     4681 tgcccgtcga atcccttgtg cagatcgcga aactggatca cagcagcacc accgcccaga
     4741 acgcgtccag caccaggatc gacatcgcgg agatcacgac ggcctgggtc gtgctccggc
     4801 ccacaccctc ggccccgccg cgcgtgcgca ggccgaggcg gcagccgacc agcgtgatga
     4861 tgagtccgaa gctggcgctc ttgaccaggg cgaaccagac atccttgaac tgatacagga
     4921 acttgaggcc cttgacgaac tcgggcgtgg acagatcgag caggctcatg ctcgtgagcc
     4981 atcccgtgac gatcccggtc acgatgccca gcaccaccac tgcgggcacc atcacggtac
     5041 cagcgagcaa cctggggacg acgaggtagg cgagggggtc gtaggccagc gtctcgagtg
     5101 cgtcgacctg ctcggggacc ttcatggtac cgagctccgc cgcgacgctg gcgccgaccc
     5161 ggccggcg
//
LOCUS       DBNGZW010000658         2168 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_403580, whole genome shotgun sequence.
ACCESSION   DBNGZW010000658 DBNGZW010000000
VERSION     DBNGZW010000658.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_403580"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..402
                     /locus_tag="AC16JC_15470"
     CDS             37..402
                     /locus_tag="AC16JC_15470"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR5166148.1"
                     /translation="MHRYRSLVAWQRSHELVILVLRTTDRCYHPRSRALFDQLRRAAL
                     SIEANIVEGYSLSTTPQFRRHLRIAIGSAAEAECFLRTAAELGYLPEPPLEEMMKLTD
                     GALRALFGLVRSLGTDRLT"
     gene            complement(436..1644)
                     /locus_tag="AC16JC_15475"
     CDS             complement(436..1644)
                     /locus_tag="AC16JC_15475"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR5166149.1"
                     /translation="MPTPSPVPRVLLVPDLPLDHAAVMDKYAHRLHDWLESGDLGFEV
                     RLAAHIGELTRAAAADGRASGGYTRWWNQPIDPSRVVLPGPLRGPQGFAARYFFYPRR
                     LARQARRVQVVHILDHRYAHLLYRARRRPVIITVHDLMPVIVLRSPTGGIAESFRNRL
                     LRRTLKALRAAETFLVGTEWLKSELATWLGDDRRIRVVPFGVDRAFFREAPGGRERGR
                     RDWRIPEEAFVVLHVGSTVDRKNVPLVIQTVARLRMEVGVDVHLLQVGGRLTADQEQL
                     VDRLGLRRVLRSVPGADESTLRRAYSAADVLLFPSLYEGFGFPVLEAFATGLPVVTSG
                     AGGLREVAGDAAVIVEGRDPAAYVQALVELSEDQDRREALIALGMARAEEFTWRRTAE
                     RTAEVYRTFF"
     gene            complement(1650..>2168)
                     /locus_tag="AC16JC_15480"
     CDS             complement(1650..>2168)
                     /locus_tag="AC16JC_15480"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5166150.1"
                     /translation="HRGLDTLLGALARHRHESWRLIVAGDGPERERLERLASELRLAA
                     RVHWVGALPPEQLAARWPSWDVLVVPSRPSSTWNEPTGTTLLEAMAHEVAVIGSDSGV
                     LPELIGSAGIVTPAGEVEPLAVAIGAILDLDRRSALARAARARALELYSDDAVAERTV
                     AFWQSLPRQKAR"
BASE COUNT          339 a          765 c          756 g          308 t
ORIGIN      
        1 gcctgaaacc tcagcaagcc cttccgacct tgaaggatgc atcggtaccg gtctctggtg
       61 gcctggcagc gatcccacga gctggtgatc ctcgtgctgc gaaccaccga ccgttgctac
      121 catccacgct cacgggcatt gttcgaccag ttgcgccgag cagcactctc gatcgaagcg
      181 aatatcgtgg aaggttattc gctgtcgacg acgccgcaat tccggcggca cctccgcatc
      241 gcgattgggt ccgcggcgga agcggagtgc tttttgagga ccgctgccga gctgggttac
      301 ctgccggaac cgccgctcga ggagatgatg aagcttacgg acggcgcttt gcgagcactt
      361 ttcggattgg tgaggagcct ggggaccgac cggttgacgt gaactccccc aaaccgctcc
      421 cccctccccg ctccctcaga agaaggttcg gtagacctca gctgttcgtt ctgccgtgcg
      481 ccgccacgtg aactcctcgg ctcgcgccat tcccagcgcg atcagcgcct cccgccggtc
      541 ctgatcctcc gacagctcga ccagcgcctg gacgtaggcc gccgggtcgc gtccctcgac
      601 gatcaccgcc gcgtcgcctg ccacctcgcg cagtcccccc gcgccggacg tgacgacggg
      661 aagacccgtg gcgaaggcct cgagtaccgg gaagccgaag ccctcgtaca gcgacgggaa
      721 gagcaggacg tcggccgcgc tgtaggcgcg tcgcagcgtg ctctcgtccg ccccggggac
      781 gctgcgcaac acgcgccgca gccccaggcg gtccaccagc tgctcctggt cggcggtgag
      841 acgcccaccg acttgcagca ggtgcacgtc cacgcccacc tccatgcgca ggcgggccac
      901 ggtctggatg acgaggggga cgttcttgcg gtccaccgtc gagccgacgt ggagcacgac
      961 gaacgcctcc tccgggatgc gccagtcgcg tcgcccgcgc tcgcggccgc cgggggcttc
     1021 gcggaagaac gcgcggtcca cgccgaacgg gaccacccgg atcctccgat cgtcgcccag
     1081 ccaggtggcg agctcgctct tcagccactc ggtgccgacg agaaacgttt cggcggcgcg
     1141 cagcgccttg agcgtgcggc gcagcagccg gttgcggaac gactcggcga ttcccccggt
     1201 gggagagcgc agcacgatga cgggcatcag atcgtgcacc gtaatgatga ccggccgtcg
     1261 gcgggcccga tacagcaggt gcgcgtagcg gtggtcgagg atgtggacga cctggacgcg
     1321 ccgggcttgg cgcgccagtc gccgcggata aaagaaatac cgggcggcga atccctgcgg
     1381 cccgcgcagc gggccgggga gcacgacgcg ggagggatcg atcggctgat tccaccagcg
     1441 cgtgtagccg cccgaggcgc ggccgtccgc cgccgcggcg cgcgtcagct cgccgatgtg
     1501 cgccgcgagg cgcacctcga aaccaagatc gccgctctcg agccagtcgt gcagccggtg
     1561 cgcgtacttg tccatcaccg ccgcatggtc caacggcagg tcggggacga gcaggacgcg
     1621 agggacagga gaaggtgtcg gcatgagggc tagcgtgcct tctgtctggg cagcgattgc
     1681 cagaacgcga cggtccgctc cgctaccgcg tcgtcgctgt acagctcgag cgcccgggcg
     1741 cgggccgcgc gcgccagcgc gctccgcctg tccaggtcga ggatcgcgcc gatcgccacg
     1801 gccagcggct cgacttcccc ggccggggtg acgatcccgg ctgacccgat gagctcgggg
     1861 agtacgccgc tgtcggagcc gatcaccgcc acttcgtgcg ccatcgcctc gagcagcgta
     1921 gtcccggtcg gctcgttcca ggtggaggaa gggcgcgagg ggacgacgag gacatcccac
     1981 gacggccagc gggcggcgag ctgctcgggg ggcagggccc ccacccaatg gacgcgcgcg
     2041 gcgagtcgca gctcgctcgc caggcgctcc aaccgctcgc gctccggacc gtccccggcc
     2101 acgatgaggc gccaggattc gtggcggtgc cgggcgaggg cgccgagcag ggtgtcgagc
     2161 ccgcggtg
//
LOCUS       DBNGZW010000659         3699 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1750999, whole genome shotgun sequence.
ACCESSION   DBNGZW010000659 DBNGZW010000000
VERSION     DBNGZW010000659.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3699)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1750999"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..960
                     /locus_tag="AC16JC_15485"
     CDS             <1..960
                     /locus_tag="AC16JC_15485"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HIR5166151.1"
                     /translation="PQAKTQLQQLGAVLIGQTPEIAPADKKLYALRDVTATVEAIPLI
                     AASIMSKKLAEGLTGLVLDVKHGSGAFLPETERGLDLARTMIALGEDRGCPTVALLTA
                     MDRPLGRACGNVLETEEAILALRGEGPEDLMEVTYALGIEMLLMAKVERTSAKARQRL
                     EDTLGSGLAAEKFERIIEAQGGNPKVVEDASGLPQAQAVEVFAAPETGVVIRVEPRTI
                     GRAIVAMGGGRTKVEDTVDPSVGFVITVKPGDKVLAGEPIASVFARDAAGIQTGFDAL
                     ARAIAIGERLAERPLPLVSHRVSRDGVETLAPLAKKATPARKR"
     gene            1011..3170
                     /gene="glgP"
                     /locus_tag="AC16JC_15490"
     CDS             1011..3170
                     /gene="glgP"
                     /locus_tag="AC16JC_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013247868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004645 - 1,4-alpha-oligoglucan
                     phosphorylase activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HIR5166152.1"
                     /translation="MTNMANRIPYLPERIGGLAAVAHNVSWSWNRDARALFRVIDQPL
                     WHLTRHNPIELLRRVDPTRLTQCAEDPEFLRRYDALMAQAGREASCEGTWFAQEYPDL
                     QDRTIAYFCAEFGLHNSVPIYSGGLGVLAGDHCKTSSDLGAPLVGVGLLYTRGYFDQR
                     VRLDGWQADADERFDASLTPLDRVVGPNGEPCLTTVPTGGRPVCVGAWRMMVGRVHVY
                     LLDTDLPQNDPADRELCQRLYGGSGELRLRQEWVLGVGGVRVLRALGNHPAVWHANEG
                     HAAFMLLERVRELVIGGTPFEDAVRQVRATSVFTTHTPVPAGHDAFALTQVEALMGPV
                     LEELKGQRDAVLRLGRAPHLAQDQFHMPVLAIRLSGRVNGVSRSHGEVSRGLWAPMWP
                     GRDQEQVPIGHVTNGVHLPTWMSHHMWELLGRHLGPGWTERIDEPGFWQCALTLDAAA
                     LWAIHNDLKVVTLRAIREMARRRWVEQWKTESLHLVGAGTLLDEHALTIGFARRFATY
                     KRASLIFRDVERLRRLLVNPWRPVQIVFAGKAHPADDPGKQMLQKVYEFTREPRFEGR
                     VAFIEDYEMHLAHRLVQGVDLWLNLPRVPLEASGTSGMKAALNGVPQLSTLDGWWHEG
                     FEGKNGWAIPPVSNGEDLDEADAERLYQVLEEQIVPLYYSRDERGVPLGWVERMKQSL
                     RVAGERFTAQRMVRQYAAQYYVPVMRGEPAADDPPTA"
     gene            3317..>3699
                     /locus_tag="AC16JC_15495"
     CDS             3317..>3699
                     /locus_tag="AC16JC_15495"
                     /inference="COORDINATES: protein motif:HMM:NF019924.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HIR5166153.1"
                     /translation="MPAIERIPAVHLTAEYWPFARTGGLGEAVNGLAEAQHAAGSPAT
                     VVMPLYRTVRETGPALERVGHQFSVSVGSRTEWAHVYRLVDRPAGPSVFFIEHPDFFD
                     RSGIYGDSGGDYPDNALRFAFFCKAA"
BASE COUNT          562 a         1261 c         1324 g          552 t
ORIGIN      
        1 ccccaggcca agacgcagct acagcagctc ggcgcggtgc tcatcggcca gaccccggag
       61 atcgcccccg ccgataagaa gctgtacgcg ctgcgcgacg tcaccgccac agtcgaggcg
      121 atacccctga tcgcggccag catcatgtcc aagaagctcg ccgaaggcct caccgggctc
      181 gtgctcgacg tgaagcacgg gagcggagcg ttcctccccg agaccgagcg ggggctcgac
      241 ctcgcgcgca ccatgatcgc gctcggcgaa gatcgcggct gtcccaccgt ggcgctgctc
      301 acggcgatgg atcgcccgct gggccgcgcc tgcggaaacg tgctcgagac cgaagaggcg
      361 atcctcgcgc tgcgcggcga agggccggag gatctgatgg aggtcaccta cgcgctcggc
      421 atcgagatgc tcctcatggc gaaggtcgaa cggacctccg ccaaggcgcg ccagcgcctg
      481 gaggacacgc tcggttcagg actcgcggcc gagaagttcg agcggatcat cgaggctcag
      541 ggcggcaacc ccaaggtggt cgaggacgcg tcggggcttc cccaggcgca agccgtggag
      601 gtgttcgccg cccccgagac gggcgtggtc atccgggtcg agccgcggac catcggccgc
      661 gccatcgtcg ccatgggcgg cggccgcacc aaggtggagg acacggtcga cccgagcgtc
      721 ggcttcgtga tcacggtgaa acccggagac aaagtgctcg cgggggagcc gatcgcgtcg
      781 gtgttcgccc gtgatgccgc gggcatccag accggattcg acgcgctcgc ccgggcgatc
      841 gcgatcggcg agcggctggc ggagcggccg ctgccgttgg tatcgcaccg cgtctcccgg
      901 gacggcgtcg agacgctggc gccgctcgcc aagaaggcca cgccggcccg gaaacggtga
      961 gaccccccga gttcccgggt cgtggcctgc ccgtgtaatt tgcgtctctc atgaccaaca
     1021 tggccaacag gattccgtac ctcccggagc ggatcggcgg cctcgcggcc gtcgcgcaca
     1081 acgtctcctg gagctggaac cgcgatgccc gggcgctgtt tcgcgtgatc gaccagccgc
     1141 tctggcacct cacgcggcac aatcccatcg agctgctgcg gcgggtggat cccacccggc
     1201 tcacccagtg cgccgaagac ccggagttcc tgcgacgcta cgatgccctg atggctcagg
     1261 ccggccgcga ggcgtcatgc gagggcacct ggttcgcgca ggaatacccc gatcttcagg
     1321 accggacgat cgcgtacttc tgcgccgagt tcgggctgca caactccgtg ccgatctatt
     1381 cgggcgggct cggtgtcctg gcgggagacc actgcaagac ctcctcggat ctcggcgcgc
     1441 cactggtggg ggtcgggctg ctgtataccc gggggtactt cgaccagcgc gtgcggctcg
     1501 acggctggca ggcggacgcc gacgagcgct tcgatgcctc gctgacaccg ctcgaccgtg
     1561 tggtcggccc caacggagag ccgtgcctca cgacggtgcc taccggcggc cggccggtgt
     1621 gcgtcggcgc ctggcgcatg atggtcggcc gcgtccacgt gtacctgctc gacaccgacc
     1681 tgccgcagaa cgacccggcc gatcgcgagc tgtgccagcg tctgtacggc gggtcgggtg
     1741 agctgcggct gcggcaggag tgggtgctcg gcgtcggcgg tgtgcgcgtg ctgcgcgccc
     1801 tcggcaacca tcccgccgtg tggcacgcca acgaggggca cgcggcgttc atgctgctcg
     1861 agcgggtgcg cgagctcgtg attggcggca caccgttcga ggacgccgtg cggcaggtcc
     1921 gggcgaccag cgtcttcacg acgcacacgc cggtgccggc agggcacgac gctttcgccc
     1981 tcacccaggt ggaagccctg atggggcccg tgctggagga gctgaaaggg cagcgcgacg
     2041 cagtgctgcg cctggggcgc gcgccgcacc tcgcgcagga tcagtttcac atgccagtgc
     2101 tcgccatccg gctgtccggc cgcgtgaacg gcgtgtcgcg gtcccacggc gaggtgtcgc
     2161 gcggtctctg ggccccgatg tggcccggcc gggatcagga gcaggtcccc atcgggcacg
     2221 tgaccaacgg ggtgcacctc cccacctgga tgtcgcacca catgtgggag ttgctggggc
     2281 ggcacctggg tcccggatgg accgagcgca tcgacgagcc ggggttctgg cagtgcgccc
     2341 tcaccctcga cgccgccgcc ttgtgggcga tccacaacga cctcaaggtc gtcacgctgc
     2401 gcgccatccg cgaaatggcg cggcgccgct gggtggagca gtggaagacg gaatcgctgc
     2461 atctggtcgg agcgggaacg ctgctggacg agcacgccct caccatcggc ttcgcgcggc
     2521 gcttcgcgac ctacaaacgg gccagcctca tcttccgcga cgtcgagcgg ctgcgccggc
     2581 tgctcgtgaa tccgtggcga ccggtgcaga tcgtgttcgc ggggaaagcc caccccgccg
     2641 acgaccccgg caagcagatg ctgcagaagg tctacgagtt cacgcgcgag ccgcgcttcg
     2701 agggacgggt ggccttcatc gaggattacg agatgcacct cgcccaccgg ctggtgcagg
     2761 gggtggatct ctggctcaat ctgccccgcg tgccgctcga ggcgtcgggc acgagcggca
     2821 tgaaggccgc cctgaacggc gtgccccagc tcagtaccct ggacgggtgg tggcatgagg
     2881 ggttcgaggg taagaacggg tgggcgatcc cgcccgtgtc gaacggggag gacctcgacg
     2941 aggccgacgc cgagcggctc taccaggtcc tcgaggagca gatcgtgccg ctttactaca
     3001 gccgggacga gcggggcgta ccgctcggct gggtggagcg gatgaagcag tccctgcgcg
     3061 tggccgggga gcggttcacg gcccagcgca tggtgcggca atacgccgct cagtattacg
     3121 ttcctgtcat gcgcggggag ccggcggcgg acgatccacc gacggcttga ttgcaccggc
     3181 cggtcaccca ccggtccctg cgcatcgtcg agaggcggtg ggtccaatga gaagtgatca
     3241 agtgaaagcg actgcgcccc ggccgtccag cgggcggcgc gtgaagtccg ggagttcccg
     3301 tcggacggcc acccccgtgc cggccatcga gcggatcccg gcggtccatc tcacggccga
     3361 gtattggcca ttcgcacgta cgggggggtt gggcgaggcc gtcaacggcc tggccgaggc
     3421 ccagcacgcg gcgggctcgc ccgcgaccgt ggtgatgccg ctgtaccgca ccgtgcggga
     3481 aaccggcccg gcgctcgagc gggtgggcca ccagttctcc gtgagcgtgg gatcccggac
     3541 cgaatgggct cacgtctacc gcctggtgga ccgccccgcc ggcccttccg tgttcttcat
     3601 cgagcatccg gatttcttcg atcgtagcgg aatctacggg gactccggcg gcgactaccc
     3661 ggacaacgcg ctgcgcttcg ccttcttctg caaggcggc
//
LOCUS       DBNGZW010000660         2885 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_879030, whole genome shotgun sequence.
ACCESSION   DBNGZW010000660 DBNGZW010000000
VERSION     DBNGZW010000660.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_879030"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="AC16JC_15500"
     CDS             <1..410
                     /locus_tag="AC16JC_15500"
                     /inference="COORDINATES: protein motif:HMM:NF036579.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-L-rhamnosidase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5166154.1"
                     /translation="GMNSFNHYAFGAVGDWMYRTIGGIDADPAAPGYRKARIAPHPGA
                     GLTHAGASLETPYGTLTSSWRRDGRQFVLEVTVPPNTSAEVTLWETEVGRIREGGRPV
                     SDAPGVHAVRRDGSNVIVTIGSGSYEFTVTSAP"
     gene            complement(459..>2885)
                     /locus_tag="AC16JC_15505"
     CDS             complement(459..>2885)
                     /locus_tag="AC16JC_15505"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HIR5166155.1"
                     /translation="VIDGRGTDQIWQGPPTIADFQEWSPTEGKPPRFPTEARIAYDRS
                     NLYVLIRCFDPHPDSIIRLIGRRDEFPQSDRVILLIDSYHDRRTGFEFGVTAAGGRLD
                     AAIYDDNNEDSAWDGVWDVATSIDSAGWTAEFRIPLSQLRYGSERRHTFGLAIDRDVY
                     RYNERVAWPLFRQSAAGMVSQFAELTGLDDLEAPRRLEAAPYVVTKNVSRVDATGFGR
                     EQEVSLGADLKYRVASNLTLDATVNPDFGQVEADPGVLNLTAYETFFPEKRPFFVSGR
                     GLFDVSINCNSVNCGSERLFYSRRIGRAPALAGIYGDATSPTSTTILGAAKLTGRLAG
                     GLRVGMLDAVTRRTAGVNDRTIEPATNYGVFRLDQDFDGGEGTVGAIVTAVNRGLDAW
                     SRPYLPAASYAGAVAFRQRLFKQTYQISALLSGSRVSGSAPVISELQRSPVHYYQRPD
                     AGLPYDTTRTVLAGDAEELKFGKVAGRHAQFETSWQRRSPGFEVNDLGYLQRAGAHNW
                     STWAGYFDRNVRRLYQSFQWNWNWWQIWTTEGLPLERAANTNVHVTLRNNWGIHFGGT
                     WGQLGATYDDRAARSGPAMRQDAYYAPWLFINGDERRQMVPSLSVNVFRGGAGHGRSL
                     SVSPGVSVKLSTRLLATASLDWSKNDNGYQWYGNVTDTLGETHYGFARLRQTTEAITT
                     RVNYTISPVMTLQMYAQPFVSKGTYSDLRELSATPRAARYDDRFQPYYDPAVAAAPGG
                     FRYMQFRSSVVFRWEYKPNSILFVVWTQGRQGLNDREGITGFGGDLNDLFDLRPDNAF
                     LVKLSYWINR"
BASE COUNT          457 a          967 c         1006 g          455 t
ORIGIN      
        1 cggggatgaa ctcgttcaat cattacgcgt tcggcgcggt gggcgactgg atgtaccgca
       61 ccatcggcgg gatcgacgcc gatccggcgg cgcccgggta ccggaaggcg cgtatcgcgc
      121 cgcacccggg tgcgggcctc acgcacgcgg gagcgtcgct cgagaccccc tacggcacac
      181 tcacctcctc gtggcggcgg gacgggcggc agttcgtgct cgaggtgacg gtcccgccga
      241 acacgtccgc cgaggtcacc ctgtgggaga cggaggtcgg ccgcatccgg gaaggggggc
      301 gcccagtgag cgacgcaccg ggcgtgcacg ccgtccgccg ggacggcagt aacgtcattg
      361 tgaccatcgg ctcgggcagc tacgagttca ccgtcacaag cgcgccgtga gtctttgccg
      421 cggcgccgga cgcgacggcg cggtctaggc ggagactcct agcggttgat ccagtacgag
      481 agcttgacca ggaacgcgtt gtccggccgc aggtcgaaga gatcgttcag gtcaccaccg
      541 aaccccgtga tgccctcccg gtcgttcagc ccctggcgcc cctgggtcca caccacgaac
      601 aggatcgaat tcggcttgta ctcccagcgg aacaccacgc tcgaacggaa ctgcatgtac
      661 cggaatccac cgggcgcggc cgccaccgcc ggatcgtagt agggctggaa ccggtcgtcg
      721 tagcgcgccg cgcgcggcgt ggccgacagc tcgcgcaggt cgctgtaggt ccccttcgag
      781 acgaacggct gcgcgtacat ctggagcgtc atgacgggcg aaatcgtgta gttgacccgc
      841 gtcgtgatcg cctccgtcgt ctggcgcagc cgcgcgaacc cgtagtgggt ctcccccagt
      901 gtgtcggtga cgttgccgta ccactggtag ccgttgtcgt tcttcgacca gtcgagggag
      961 gccgtggcga ggaggcgggt ggacagcttg acgctcaccc cggggctcac cgagagcgag
     1021 cggccatgac ccgcaccgcc ccggaacacg ttgacggaga gcgacggcac catctgccgg
     1081 cgctcgtcac cattgatgaa gagccacgga gcgtaatagg cgtcctgccg catcgcgggc
     1141 ccgctgcgcg cggcgcggtc gtcataggtg gcgccgagct gcccccaggt cccgccgaaa
     1201 tggatgcccc agttgttccg cagagtgacg tgcacgttgg tgttggccgc ccgctcgagc
     1261 ggtagccctt ccgtggtcca gatctgccac cagttccagt tccactggaa gctctgatac
     1321 agccggcgca cgttgcggtc gaagtacccg gcccaggtgg accagttgtg cgcgccggcc
     1381 cgctgcagat atcccaggtc gttgacctcg aagccgggcg agcggcgctg ccaggacgtc
     1441 tcgaactggg cgtgccggcc cgccaccttg ccgaacttca gctcctcggc gtcgcccgcg
     1501 aggacggtcc gggtcgtgtc gtaaggcagg ccggcgtccg gtcgctgata gtagtgcacc
     1561 gggcttcgct ggagctccga aatcaccggt gcactgcccg atacgcggct cccggacagc
     1621 agggccgaga tctggtaggt ctgtttgaag agccgctggc ggaaggcgac cgccccggcg
     1681 taggaggcgg cggggaggta cggcctgctc cacgcgtcga ggccgcgatt gaccgccgtg
     1741 acgatggccc cgacggtccc ttcccctccg tcgaagtcct ggtcgaggcg gaacacgccg
     1801 tagttggtgg ccggctcgat ggtccgatcg ttcacgcccg ccgtccgccg cgtcaccgcg
     1861 tcgagcatcc cgactcggag accccccgcc aggcgcccgg tgagcttggc ggcaccgaga
     1921 atcgtggtag aggtcggtga cgtggcgtca ccgtagatgc ccgccagcgc cggcgcccgg
     1981 ccgatgcgac ggctgtaaaa cagccgctcg ctgccgcagt tcacgctgtt gcagttgatg
     2041 gagacgtcga acaacccacg ccccgagacg aagaacgggc gcttctccgg gaagaacgtc
     2101 tcataggccg tgaggttgag cacgccggga tcggcttcga cctggccgaa gtcgggattg
     2161 accgtggcgt cgagggtcag gttcgaggcc acgcggtatt tcagatcggc gccgagcgag
     2221 acctcctgct cgcggccgaa acccgtcgcg tcgacgcgcg acacgttctt ggtgacaacg
     2281 tacggcgccg cctccaaccg ccgcggggct tccaggtcgt cgaggcccgt caactcggcg
     2341 aactgcgaga ccattcccgc cgccgactgg cggaacagcg gccaggccac ccgctcgttg
     2401 tagcggtaga cgtcgcgatc gatggcgagc ccgaaggtgt gccgtcgctc cgacccgtaa
     2461 cgcagctgcg acagggggat gcggaactcc gccgtccagc cggccgagtc gatgctcgtg
     2521 gccacgtccc acaccccgtc ccatgcggag tcctcgttgt tgtcgtcgta gatcgccgcg
     2581 tccaggcgcc cgccggccgc ggtgacgccg aactcgaatc ccgtgcggcg atcgtgatag
     2641 gagtcgatca gcaggatgac ccggtcggac tggggaaact cgtcgcggcg cccgatcagc
     2701 cggatgatgc tgtccgggtg ggggtcgaag cagcggatca ggacgtacag gttcgagcga
     2761 tcgtaggcga tcctcgcttc ggtgggaaac cgcggcggct tcccttccgt gggggaccac
     2821 tcctggaaat cggcgatcgt gggggggccc tgccagatct ggtcggtgcc gcggccgtcg
     2881 atgac
//
LOCUS       DBNGZW010000661        12073 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_61691, whole genome shotgun sequence.
ACCESSION   DBNGZW010000661 DBNGZW010000000
VERSION     DBNGZW010000661.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 12073)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12073)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12073
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_61691"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="AC16JC_15510"
     CDS             complement(<1..358)
                     /locus_tag="AC16JC_15510"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF012915.7"
                     /note="binds the polymerase to DNA and acts as a sliding
                     clamp; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR5166156.1"
                     /translation="MKFTITREQLQEGLTAVAAAIPAKTTLPVLSNVLVEATKQGLRL
                     SGTDLDIAVSTTVAADVDTEGAVTLPAKKLSDIVRELPSSPIRFTGAGEQRVGIDCCR
                     SRFKLLGLPREEFPSFP"
     gene            complement(601..1974)
                     /gene="dnaA"
                     /locus_tag="AC16JC_15515"
     CDS             complement(601..1974)
                     /gene="dnaA"
                     /locus_tag="AC16JC_15515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HIR5166157.1"
                     /translation="MEQSAKEAWKRLLDEARRELPEATVRTWLEPAVPLALEEGRLVL
                     GTTDQFAAEWNDTKHAPLLTRAAERVFGQPTPVVFRVMEERRQRPQMDFFVAPKPIPV
                     TAPTVTMPLNERYTFESFVVGKSNELAAAAAHAVAEAPGRTYNPLFIYGATGLGKTHL
                     MQAIAHAVLKRNPETRLLYVGAEQFINEVIESIQARTMPEFRRRYRNDIDLLLVDDVH
                     FLEGKEMTQDEFFHTFNALFEGRKQMVLTSDRPPKEIPGLEDRLISRFEWGMVADIGQ
                     PDLEHRIAILRKKAYQDHLELTIPDDVLRFIAEHIRSSVRELEGCIIKLLLFASLKNR
                     EVTIDLAREALADKIRQGEESAFPGPATPSIDRVQEVVARRWGVTPEGLRSKARTKTL
                     TIPRQVAMYLARDMLGMQLVEIGQAFGGRDHSTVIHSVDKVERQMMRDRTFKERIEMA
                     RQELSAL"
     gene            2103..2267
                     /gene="rpmH"
                     /locus_tag="AC16JC_15520"
     CDS             2103..2267
                     /gene="rpmH"
                     /locus_tag="AC16JC_15520"
                     /inference="COORDINATES: protein motif:HMM:TIGR01030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HIR5166158.1"
                     /translation="MGKPTYRPRNRRRINKHGFRARMKDKWGRATLGRRRKKGRKRLT
                     VQLPSKHRSG"
     gene            2285..2608
                     /gene="rnpA"
                     /locus_tag="AC16JC_15525"
     CDS             2285..2608
                     /gene="rnpA"
                     /locus_tag="AC16JC_15525"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR5166159.1"
                     /translation="MRVRLPRGPDLTQCWESGRRVRMRRLDVAWRPNSQGHARAGIVV
                     PRFGETAVARNRLRRRMREIMRRDLLRVLPAIDLVVRAKPAAYGARFAELRAELIGAV
                     ERMRP"
     gene            2638..2832
                     /gene="yidD"
                     /locus_tag="AC16JC_15530"
     CDS             2638..2832
                     /gene="yidD"
                     /locus_tag="AC16JC_15530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017663686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR5166160.1"
                     /translation="MGMIRAYQLTLSHLMFTQCRFTPSCSRYAYEAVERYGALRGSWL
                     GVRRILRCHPFHPGGYDPVP"
     gene            2842..4530
                     /gene="yidC"
                     /locus_tag="AC16JC_15535"
     CDS             2842..4530
                     /gene="yidC"
                     /locus_tag="AC16JC_15535"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HIR5166161.1"
                     /translation="MDQRRVIAAILLMLVIAIVPSIIFKPKPVPRAPGAAPDTGRAAI
                     APVESAPAAVVPAPPAHPPIRPSAQLPAETVWVTSPLYRLGFTTRGARLVTAELEQYR
                     SFARGDSGSRVQLVPGDAPLLGLRLVAGSDTVSLDALAFSPDRAALSVAGADTTLTFT
                     APLGSGRATLSYRFMPGHYRFAVDGRIDGLGGTGAVLLVDLGNGLRSVEADPMTDYRE
                     YAIVTKADKTQKTTFASLDPGERAVLNGPFEWVALKSKYFFTAIMAPEQNVRPFAGAV
                     ATGMPRTPVPTASLFGTRTSALATQASVITTLALPPAGTFRFEVYTGPFEHQRLSQLG
                     HGLDDANPYGWSIFRPIIHPVSLVVVEILFWMHERLHLAYGWVLVFFGVLIRLLLWPL
                     QQKAMESQMRMQAVQPLMQEIQTRYKNEPEKLQREMMRLYKEHKVNPFGGCLPMLLPM
                     PVLLALFFVFSNTIEFRGVPFLWLPDLSRHDPLYIIPVVMGLSMFAVSKIGQRGMPPN
                     PQMKTMLYIMPAMMTFIFLRLASGLNLYYAVQNLISLPQQWQISQKRLAAQKRS"
     gene            4553..5929
                     /gene="mnmE"
                     /locus_tag="AC16JC_15540"
     CDS             4553..5929
                     /gene="mnmE"
                     /locus_tag="AC16JC_15540"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HIR5166162.1"
                     /translation="MLSDPIVALATPAGRGALALIRLSGRGAFELAARVLRPFRPNLP
                     RRVVRADVHHPHSGEPIDDVLAVAFPGPASYTGEDMVEISTHGGLLVPAEVVAACVAA
                     GARPAAPGEFTRRAVQNGKMDVLQAEATADLIDAGAPAQRRRALQQLERGLSRRLEAL
                     REEILELEALIAYDIDFPEEDEGPVGPERIERAWRVARDRVARLLATAPQGERLHAGA
                     LVVIAGPPNAGKSSLFNALLGRERAIVTEIPGTTRDAIEAQAAIDGLPFRLVDTAGLR
                     VSDDRVEQLGIEVSRRYLGAADLILFCEAGDAGRGTGDEPAREAFLAQCSAPVVRVRT
                     KADLSGPTARPPDRLTADRPSVPVSVLTGEGLPELGRRLVEAAFSGLVALGDVEPVVT
                     RERHRVALERALEELDLFWTAHGQGVDAAAAATHLRAAVGALDDLIGVVTPEDVLDRV
                     FASFCVGK"
     gene            complement(5932..6276)
                     /locus_tag="AC16JC_15545"
     CDS             complement(5932..6276)
                     /locus_tag="AC16JC_15545"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014430207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HIR5166163.1"
                     /translation="MATETPYTDAQVRERLAALPGWHYEGGMIRRRIKTDGWPTTLML
                     VNAIGYYAEAADHHPDLMVQWGSVQVSLATHSAGGVTEKDFALAEVVERAALWQAGSP
                     FRPASKFRREGA"
     gene            complement(6279..6791)
                     /locus_tag="AC16JC_15550"
     CDS             complement(6279..6791)
                     /locus_tag="AC16JC_15550"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010049340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR5166164.1"
                     /translation="MHDSGSGFKVSVRKEDLVFAAAHFITLPGHRCEALHGHNYRAGV
                     ELTGTLEPDAWYVFDFVALKQVMRRLTNELDHKVLLPTTNPTVTVQANGDSVAVTYDG
                     RPRYVFPAGDCVLLPIPNTTVEMLAEYLAGRLKAELKTKGAAQVSGLEIEVEENFGQS
                     ASYREALSWD"
     gene            complement(6834..7043)
                     /locus_tag="AC16JC_15555"
     CDS             complement(6834..7043)
                     /locus_tag="AC16JC_15555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166165.1"
                     /translation="MTPRGGAPSERRHNYTLREILEELVGHARDIASRAKSMSPEELE
                     RAQQRLEWLADEVWRVATEQLPPPE"
     gene            complement(7040..7999)
                     /locus_tag="AC16JC_15560"
     CDS             complement(7040..7999)
                     /locus_tag="AC16JC_15560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HIR5166166.1"
                     /translation="MQPGPMFVNLVPEFLACLAAPDPVAAYHSYLERHGPVLASYWHN
                     YVLDLESPHAERVIRETVRADRDDLRRLLEDVDVARIADEALTRARDQLEADCPTDLY
                     LMVGVGAANAGELVVDGRGVAFVCVEHFTGRANPETNGLGLAPHLLPLWIAHEIAHAV
                     RYTSPTSKAEMRRLVAEGEGTYDYWTTGSRATLRELLVNEGIAVAASQAVAPGFEPWE
                     YLGYGRRQYRRIRELDAFLRRAAAAELDRRGLGLRLRFLSGGMSPASRLVNGKVLPER
                     SGYYLGLRMVESHVTERGLGATVRAPAGEIQQAEDRASGIQTA"
     gene            complement(8039..9997)
                     /locus_tag="AC16JC_15565"
     CDS             complement(8039..9997)
                     /locus_tag="AC16JC_15565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166167.1"
                     /translation="MLLGLLLLLQQDSVHTRIETLLAAHDLPAARRIAEHLVAAHPRD
                     PRAHLLLGRVWYAWPAIGRYTALAEFRNAARLAPEDPLPLYWQVRVGQHLGSDEGEVI
                     IREAILRIFAVTPDYEDCWALFRGLYHSPDIWRRADRALARHPDDPVALERRARIAIA
                     LERPGQADSLAERAMGQRGPAVPLLLLRAEAAFDAARDSAGYAWYDSALARADGDSTG
                     ALWDQVWMIASPAEAARQGATVPGRRKDFFAWFWGRRDPDLVTAQNERIAEHFRRMAE
                     VRRMFHLLHPYVAWHRSPSARALAASYDRDGFLAVAESGTVLDQYSPARLLMTDVRDF
                     NDTIGQASVYALANLSARGLVWLRHGRPDYWDREQGSFFATHEWTYYTADGPLTIGFE
                     GIPGALGAHGDQIVAPPVNRHEARQVRTLLTTDQTSLPATLVARGWSAFFLDGGSGYT
                     DLYVKAAPGTAAAVLRDTVAEGEIGRARGGLLHLAAPPGLYHLALDVDSGGAVGRIRE
                     LVRLPAFSWATLGVSSLVLAPGDSLSTRDATLAGMPADLSYAAGRAVATYAELYGLSR
                     DSDGRGRYRVRYSFAPVRSLAARLTRGTSPVVFEFDRESEWHGTTPERLVIEPGRLPP
                     GRYRVTLSVTDLPTNVKSETVALVITIR"
     gene            complement(10002..10853)
                     /locus_tag="AC16JC_15570"
     CDS             complement(10002..10853)
                     /locus_tag="AC16JC_15570"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR5166168.1"
                     /translation="MIPERTDGVTPWVGRLLVANLIAYLLKSTLFWAPRFLALFGFSP
                     LAVWAHPWTLVTYQFVHASILHLAFNLVALYFFGPPVEARLGGRRFIGFYILCGIGGA
                     VLSALLVPLFGDQLVVGASGAVLGVAVAFAWYWPDAPVQVFPFPEPFAAKWLVTFTVA
                     ISLALAWLSAATGLSDGVSHLAHLGGIGTAFLVLKAADWRMTRHEQRLRRNSVPNAVV
                     SSAALVARSSDPERRSPAPRRRPADRTQAEIDRVLDKISAGGINSLTPAERKFLAEMS
                     RRMRDQS"
     gene            complement(10859..11881)
                     /locus_tag="AC16JC_15575"
     CDS             complement(10859..11881)
                     /locus_tag="AC16JC_15575"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012853777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR5166169.1"
                     /translation="MRITVLTGGATAERTVAFAGAAQVVAALRGRGHQVHVVDIASGV
                     LTAAAEARLLVPHVGTVLPDTSELIADERRMLCGGLAGLAVVQGADVVFPVVHGGAGE
                     GGLLQAVLEMIGVPYTGSGPLGSALAVDKDLSKRLFRDAGVPVPEWRMTPPAEPADAA
                     AELGWPVIVKPSKQGSTVGLTLVKRKAGLATAVAEAVRWDDEVMIEQFIPGRELTVGV
                     LGDEALPVGEIVPRHELFDYECKYTPGMSEEIFPAALEPPIARRVQALAVVAHRALKP
                     GGYSRIDFRLGAEGDIFCLEANNLPGMTGTSLFPQAARAAGIEYPDMCERICRLARNF
                     RVGRRE"
     gene            complement(11882..>12073)
                     /locus_tag="AC16JC_15580"
     CDS             complement(11882..>12073)
                     /locus_tag="AC16JC_15580"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5166170.1"
                     /translation="AVLDQAVRDLMDPPFPVVDVDWPLDRLTALLSRETPAVLVRRDR
                     ELTGIVTRYDLLHQLAGIR"
BASE COUNT         1845 a         4184 c         4113 g         1931 t
ORIGIN      
        1 cggggaacga ggggaactcc tcccgcggca gtcccaggag cttgaagcgg gagcggcagc
       61 agtcgatgcc cacgcgctgc tccccggcgc cggtgaaccg gatcggcgac gaaggcagct
      121 cccgcacgat gtcgctcagc ttcttcgcgg gcagcgtgac cgcgccctcg gtgtccacgt
      181 ccgcggccac ggtggtgctg acggcaatgt cgaggtcggt gccggagagg cggagaccct
      241 gcttggtcgc ctcgacgagc acgttcgaca gcactggcag cgtcgtcttg gcagggatcg
      301 cggccgcgac ggcggtcagg ccttcctgca gctgttcccg cgtgatcgtg aacttcatgc
      361 ccgtgactcc gctcctcctg cagaggtgag aatcctactg ctgtactgta tatgaaatac
      421 tagtagtagt agtagaggcc ggggatatgt gggtgtttac cccaaacccc tccggacaca
      481 gcaccttgac ccggttccac gctgttggcg ctttgtgggc gagcggcggc tttcccacag
      541 tctcccacac cgcgtgcgtg tcgtcccaca gtcccccaca tacgccgccc cgaatgttgg
      601 ctacagtgcg gacaattcct ggcgcgccat ttcgatccgc tccttgaacg tccggtcccg
      661 catcatctgg cgctcgacct tgtccacgct gtggatcacc gtggaatggt cccggccccc
      721 gaacgcctgg ccgatctcca ccagctgcat ccccagcatg tcccgcgcga gatacatggc
      781 gacctggcgc gggatggtga gcgtcttggt gcgggccttg gaccggagac cctccggggt
      841 gacgccccag cgccgcgcga ccacctcctg tactcggtcg atgctgggag tggcggggcc
      901 ggggaacgcg ctctcctccc cctgccggat cttgtcggcc agggcctcgc gcgccaggtc
      961 gatcgtcacc tcccggttct tcagggaggc gaacagcaac agcttgatga tgcacccttc
     1021 gagctcccgg acgctcgagc ggatgtgctc ggcgatgaac cgcagcacgt cgtccggaat
     1081 ggtgagctcg aggtggtcct ggtacgcctt cttccgcagg atggcgatgc ggtgctcgag
     1141 gtcgggctgg ccgatatcgg ccaccatccc ccactcgaaa cgcgagatca gccggtcctc
     1201 gaggcccggg atctccttcg gcggccggtc cgaggtgagc accatctgct tgcggccctc
     1261 gaacaacgca ttgaacgtgt ggaagaactc gtcctgggtc atctccttgc cttccaggaa
     1321 atgcacgtcg tccaccagga ggagatcgat gtcgttgcga tagcggcgcc ggaactccgg
     1381 catggtccgg gcctgaatgc tctcgatgac ttcgttgatg aactgttcgg ccccgacgta
     1441 gagcaggcgg gtctccggat tacgcttcaa caccgcgtgc gcgatggcct gcatcaggtg
     1501 cgtcttgccg aggccggtcg cgccgtaaat gaagagcggg ttgtaggtgc ggccgggtgc
     1561 ttccgccacc gcgtgggccg cggcggccgc cagttcgttg gatttgccga ccacgaagct
     1621 ctcgaacgtg tagcgttcgt tcagcggcat tgtgacggtg ggcgccgtga cgggaatcgg
     1681 cttcggcgcc acgaagaaat ccatttgggg acgctggcgc cgctcctcca tcacccggaa
     1741 cacgaccggt gtcggttggc cgaacacgcg ttcggcggcg cgcgtcagca gcggcgcatg
     1801 cttcgtgtcg ttccactcgg cggcgaattg atccgtggtg cccagcacca gacgcccctc
     1861 ctccagcgcc agcggcaccg ccggctcgag ccaggtgcgc accgtcgctt cgggaagctc
     1921 acgtcgcgcc tcgtcgagga ggcgcttcca ggcttctttc gctgactgct ccatcaaagg
     1981 gcgataattt tggtgggacg tattgcaagg gcggggcaaa actatggccc catgtggaaa
     2041 agtcaacctt gaacgcacct cccccggcat gtaggtttcc ccgccttact tcagaggcat
     2101 gtatggggaa gccgacctac cgcccgcgta atcggcggcg catcaataag catggattcc
     2161 gcgctcgcat gaaagacaaa tggggccgtg cgacactcgg gcggcgccgc aagaaggggc
     2221 ggaagcgcct cacggtccag ctcccgtcca agcatcggag cggttgacgc gcgagcggta
     2281 tccggtgcgt gtgcgactgc cgcgcggccc ggacctcacg caatgctggg agagcggtcg
     2341 tcgggtgcgg atgcgccggt tggacgtcgc ttggcgtccc aattcgcagg gccacgcgcg
     2401 ggcggggatc gtcgtccccc gtttcggaga aaccgcggtg gcccgcaatc gtttgcggcg
     2461 ccgcatgcgt gagatcatgc gacgcgatct gctccgggtc ctacccgcaa tcgacctggt
     2521 cgtgcgggcg aaaccagccg cctacggcgc ccgttttgca gagttgcgcg ccgagttgat
     2581 cggcgccgtc gagcggatgc gtccgtgaat cccgctgtct ggccgcggct cgcggtgatg
     2641 ggcatgattc gcgcgtacca gttgacgctg tcacatctga tgttcaccca gtgtcggttc
     2701 acgccgtctt gctcgcgcta cgcgtacgag gcggtggaac gatacggcgc gctgcgggga
     2761 agttggctcg gcgtgaggcg gattttgcga tgccacccgt ttcatccggg tggctacgac
     2821 ccggttccct aggacgcacc cgtggatcag cgacgcgtca tcgcagcgat cttgctgatg
     2881 ctcgtcatcg ccatcgtgcc gagcatcatc ttcaagccga agccggtgcc ccgcgccccc
     2941 ggcgcggcgc cggataccgg tcgggctgcg atcgcaccgg tggaatccgc gccggcggcc
     3001 gtggtaccgg caccacccgc ccatccgccg atccgcccat ccgcccagct ccccgctgag
     3061 acggtctggg tcacgagtcc gctgtaccgt ctcgggttca ccactcgggg cgctcgcctg
     3121 gtcacggccg agctcgagca gtaccggtcg ttcgcgcgcg gggattcagg gagccgggtc
     3181 cagctcgttc ccggcgatgc cccgctgctg gggctgcgcc tcgtggcggg gtccgacacg
     3241 gtctcgctcg acgcgctcgc gttctcgccg gaccgcgccg cgctgtcggt cgccggcgcc
     3301 gataccaccc tcacgttcac ggcgccgctc ggctccgggc gcgccacgtt gagctaccgg
     3361 ttcatgcccg gccactaccg ctttgccgtg gatgggcgca tcgacgggct ggggggcacc
     3421 ggcgcggtcc tgctcgtgga tctgggaaac ggcttgcggt cggtcgaggc cgacccgatg
     3481 accgactacc gcgagtatgc gatcgtcacc aaggcggaca agacccagaa gaccacgttc
     3541 gcttcgctcg atcccgggga acgcgcggtc ctgaacgggc cgttcgaatg ggtggcgctg
     3601 aaatcgaagt acttcttcac ggccatcatg gcgccggagc agaatgtccg cccgttcgcc
     3661 ggcgcggtgg cgaccgggat gccgcgcacc ccggtgccca cggcgtcgct gttcggcacc
     3721 cggacgtccg cgctcgcgac ccaggcgtcg gtcatcacga cgctggcgct gccgcccgcg
     3781 ggcaccttcc gcttcgaagt gtacacgggt ccgttcgagc accagcggct gtcgcagctc
     3841 ggccatgggc tggacgacgc caatccgtac ggctggtcga tcttccggcc gatcatccac
     3901 cccgtgtcgc tcgtcgtcgt cgagattctc ttctggatgc acgagcgcct gcacctcgcg
     3961 tatggctggg tgctcgtgtt tttcggtgtg ctcatacgcc tgctgctgtg gccgctccag
     4021 cagaaggcca tggagtcgca gatgcgcatg caggcggtgc agccgctgat gcaggagatc
     4081 cagacgcgct acaagaacga gccggagaag ctgcagcgcg agatgatgcg gctctacaag
     4141 gagcacaagg tcaacccgtt cggcggctgc ctgccgatgc tgctgcccat gccggtgctg
     4201 ctcgccctgt tcttcgtgtt ctccaatacc atcgagttcc gaggcgtgcc gttcctgtgg
     4261 ctcccggatc tctcgcgcca tgacccgctc tacatcatcc cggtcgtgat ggggctctcg
     4321 atgttcgcgg tgtcgaagat cggccagcga ggcatgccgc ccaatcccca gatgaagacc
     4381 atgctgtaca tcatgcccgc gatgatgacg ttcatcttcc tgcgcctggc ttcgggactg
     4441 aatctctact acgccgtcca gaacctgatc agcctgccgc agcagtggca gatctcccag
     4501 aagcgcctgg cggcgcagaa gcgctcctga gcgggcgcac cgggggaaca gggtgctgtc
     4561 cgacccgatc gtcgccctcg ccacaccggc cggtcgcggc gccctcgcgc tgatccggct
     4621 ctcgggccgc ggggcgttcg agctcgcggc ccgtgtactg cgcccgtttc gcccgaatct
     4681 cccgcgccgc gtcgtccgcg ccgacgtcca ccacccgcac agcggcgagc cgatcgacga
     4741 cgtcctcgcc gtggccttcc ccggtcccgc ctcgtacacc ggggaggaca tggtcgaaat
     4801 ctcgacccac ggcggcctgc tggtgccggc cgaggtcgtc gccgcttgtg tggctgctgg
     4861 ggcccggccg gccgcccccg gcgaattcac ccgccgggcc gtgcagaacg gtaagatgga
     4921 tgtgctccag gcggaagcca ccgccgatct cattgacgct ggggcgccgg cgcagcggcg
     4981 gcgcgccctc cagcagctcg agcgcggcct gtcgcgccgc ctcgaggcct tgcgggagga
     5041 gatactcgag ctcgaagcgt tgatcgccta cgacatcgac ttccccgaag aagacgaagg
     5101 gccggtcggc ccggagcgga tcgagcgggc ctggcgcgtg gcccgggacc gggtggcccg
     5161 cctgctggcc accgccccgc agggggagcg gctgcacgcg ggggcgctcg tcgtcatcgc
     5221 cggaccgccg aacgccggaa agtcgtcgct cttcaacgcg ctgctcgggc gggagcgcgc
     5281 catcgtcacc gagataccgg gcaccacccg cgatgcgatc gaggcgcagg ccgccatcga
     5341 cgggctgccg ttccggctcg tggacacggc gggcctgcgg gtcagcgacg atcgtgtgga
     5401 gcagctcggc atcgaagtga gccggcgcta cctcggcgca gcggatttga tcctcttctg
     5461 tgaggcgggg gacgcgggac gagggacggg ggacgagccg gcgcgcgagg cgttcctcgc
     5521 gcagtgctcg gctcccgtgg ttcgcgtccg gacgaaggca gacctctctg gaccgaccgc
     5581 ccgaccgcct gaccgcctga ccgccgaccg cccgtccgtc cccgtctccg tcctgaccgg
     5641 ggaaggactg cccgagttgg gccgtcgcct cgtggaagcg gcattcagcg ggctggtcgc
     5701 gctgggggac gtggaacccg tggtgactcg ggagcgtcac cgagtggctc tggagcgcgc
     5761 gctcgaggag ctcgacctct tctggacggc ccacgggcaa ggcgtggatg cggcggcagc
     5821 ggcgacgcac ctgcgcgccg cggtgggggc attggatgac ctgatcgggg tcgtgacccc
     5881 ggaggacgtg ctggaccggg tgtttgcgag cttctgcgtg gggaagtgag ctcaggctcc
     5941 ttcgcgccgg aacttggacg ccggacggaa cggagatccg gcctgccaca acgcggcgcg
     6001 ctcgacgacc tcggcgagcg cgaagtcctt ctccgtcact ccgcccgcgc tgtgcgtcgc
     6061 gagtgacacc tgcaccgagc cccactgcac catcaggtcg gggtgatggt ccgcggcctc
     6121 cgcatagtac ccgatcgcat tcacgagcat cagcgtggtc ggccagccgt ccgtcttgat
     6181 cctgcgacgg atcatcccgc cttcgtagtg ccagccggga agggcggcca ggcgctcgcg
     6241 gacctgcgcg tcggtgtagg gggtctccgt ggccacggct agtcccagct cagcgcttcc
     6301 cggtaactcg ccgactgccc gaaattctct tcgacttcga tctcgaggcc cgaaacctgg
     6361 gccgcccctt tcgtcttgag ctcggccttg agccgcccgg cgagatactc ggcgagcatc
     6421 tccaccgtcg tgttgggaat cggcagcagc acgcagtcgc cggccggaaa cacatagcgc
     6481 gggcggccgt cgtaggtcac tgcgacgctg tcgccgttgg cctgcaccgt gaccgtcgga
     6541 ttggtggtgg gaagcaggac cttgtggtct agctcgttgg tgagccgccg catcacctgt
     6601 ttcaacgcca cgaagtcgaa gacgtaccac gcgtccggct cgagcgtccc ggtcaactcg
     6661 acaccggcgc ggtagttgtg accgtgcagg gcttcacaac gatggcccgg caacgtgatg
     6721 aagtgcgcgg cggcgaagac gaggtcttcc ttgcgcaccg ataccttgaa cccgctcccg
     6781 gagtcgtgca tggtgccggg aagatatagt gcggcgcacc ggccgccgcg gggttactcg
     6841 ggcggcggca gctgctcggt cgccacgcgc cacacctcgt ccgccagcca ctccaggcgt
     6901 tgctgagcgc gctccaactc ctctggactc atggacttcg cgcggctcgc gatgtcgcgc
     6961 gcgtggccca ccagctcctc gagaatctcc cgcagcgtgt aattgtgtcg ccgctccgac
     7021 ggggcgccgc cgcgcggggt catgcggtct ggatgccgct cgcgcggtcc tcggcctgct
     7081 ggatttcccc ggcgggagcc cgcaccgtgg ccccaagtcc gcgctccgtg acgtggctct
     7141 ccaccatgcg caatccgagg tagtagcccg accgctccgg cagcaccttc ccgttcacga
     7201 gccgcgaggc cggactcatc ccgccggaga ggaatcgcag gcgtagaccc agaccgcggc
     7261 gatccagctc cgccgcggct gcgcgccgca ggaacgcgtc gagctcgcgg atgcggcgat
     7321 actgccggcg gccgtagccc aggtattccc atggctcgaa tcccggcgcc accgcctgcg
     7381 aggcagccac cgcgatgccc tcgttgacca gcagctcacg gagcgtcgcc cggctgcccg
     7441 tggtccagta gtcgtacgtt ccttctcctt cggcgaccag acggcgcatc tcggctttgc
     7501 tggtcggcga agtgtaccgc acggcgtggg cgatctcgtg agcgatccac aacggtagga
     7561 gatggggagc cagccccagc ccgttggtct cggggttcgc ccggccggtg aagtgctcca
     7621 cacacacgaa ggcgactccc cggccatcca ctaccagctc cccggcgttg gccgcgccga
     7681 cgccgaccat gaggtagagg tcggtggggc agtcggcctc gagctggtcc cgggcgcgcg
     7741 tcagcgcctc gtcggcgatg cgcgcgacgt cgacatcctc gagcaagcgg cgcaggtcgt
     7801 cgcgatccgc gcgcaccgtc tcccggatga cccgctccgc atgcggcgac tcgagatcga
     7861 gcacgtagtt gtgccagtag ctcgccagca ccggcccatg acgttcgaga taggagtggt
     7921 acgccgcgac cggatccggg gcggcaaggc aggcaagaaa ttccgggacg agattgacga
     7981 acattggacc gggttgcacc ccaatttggg agcgttcttc cggcccgaca agtctggctc
     8041 agcggatggt gatgacgagc gcgacagtct ccgacttgac atttgtcgga aggtcggtca
     8101 ccgacagcgt cacgcgatac cgtccgggcg gcagtcgccc gggctcgatc accagtcgct
     8161 ccggcgtggt cccgtgccac tcgctctcgc ggtcgaattc gaacaccacc ggcgacgtcc
     8221 cgcgcgtcag ccgtgcggcg agcgagcgca ctggcgcgaa actgtagcgg acgcggtagc
     8281 ggcctcgccc gtcgctgtca cgagacagtc cgtacagttc ggcgtaggtg gccacggcgc
     8341 gacccgccgc gtagctgagg tcggcgggca tccccgccag cgtcgcgtcg cgggtgctca
     8401 gcgaatcgcc cggcgccagc acgagactcg atacgccgag ggtcgcccag gaaaaggccg
     8461 ggagccggac gagttcgcgg atgcgcccca cggcgcctcc cgaatcgacg tccagcgcga
     8521 ggtggtacag tcccggtggc gcggcgagat gcagcaatcc accccgggct cggccgatct
     8581 cgccttcggc caccgtgtct cgcagcaccg cggcggccgt gccgggtgcc gccttcacgt
     8641 acagatccgt gtagccgctt cctccatcca ggaaaaaggc gctccacccc cgggcaacga
     8701 gcgtcgcggg gagactcgtc tggtcggtag tgagcagcgt ccggacctgg cgcgcctcgt
     8761 gccggttcac gggcggggcc acgatttggt ccccgtgcgc gcccagcgcg ccgggaatcc
     8821 cctcgaagcc gatcgtgagt gggccgtcgg ccgtgtagta cgtccactcg tgggtcgcga
     8881 agaagctgcc ctgctcgcga tcccagtagt cgggtcgccc gtgccgcaac cacacgagac
     8941 cgcgggcgct caggttggcg agggcgtaca cgctcgcctg gccgatcgtg tcgttgaagt
     9001 cccgcacgtc tgtcatcagc agccgcgccg gggaatattg atcgagtacc gtgccgcttt
     9061 ccgcgaccgc caggaatccg tcccggtcat aggaggcggc gagcgccctg gcggatggag
     9121 aacggtgcca ggccacgtac ggatgcagca ggtgaaacat ccgtcgcacc tccgccatcc
     9181 gccggaagtg ttcggcgatg cgctcgttct gcgcggttac gagatcgggg tcgcgccgtc
     9241 cccagaacca cgcgaagaag tccttgcgcc gccccgggac cgtggcgcct tgtcgggccg
     9301 cctcggccgg cgacgcgatc atccacacct ggtcccacag ggcgccggtc gagtcgccgt
     9361 cggcgcgcgc gagcgcggag tcgtaccagg cgtaccccgc gctgtctcgc gctgcgtcaa
     9421 acgccgcctc cgcgcggagc aggaggagag ggaccgccgg cccgcgctgg cccatggccc
     9481 gctccgcaag cgagtccgcc tgccccggtc gctcgagcgc gatggcgatg cgcgcccggc
     9541 gctccagcgc cacgggatcg tccgggtgcc gagcgagcgc ccggtcggcc cgtcgccaga
     9601 tgtcgggact gtggtacagt ccccggaaca gcgcccagca gtcctcgtag tcgggcgtca
     9661 ccgcgaagat ccgcagaatg gcctcgcgta tgatcacctc gccttcgtcg ctccccaggt
     9721 gctgtccgac ccgcacctgc cagtagagcg gcagcggatc ctcgggggcg agtcgcgcgg
     9781 cgttgcggaa ttcggcgagc gccgtgtagc gcccgatcgc gggccaggcg taccacacgc
     9841 gacccaggag gaggtgggcc cgggggtccc ggggatgtgc ggcgacgagg tgctcggcga
     9901 tccggcgcgc ggcgggcagg tcgtgggccg ccagcagggt ttctatgcgg gtgtgaaccg
     9961 agtcctgttg caggaggagc agcaggccga gcaacatcca ctcagctctg gtcgcgcatc
    10021 cttcggctca tctcggccag gaacttccgt tccgcgggcg tgaggctgtt gatgccgccg
    10081 gccgagatct tgtcgaggac gcgatcgatt tccgcctggg tgcggtcggc cgggcgtcgg
    10141 cgcggcgcgg gactgcgccg ttcgggatcg ctcgaacggg ctacgagcgc cgccgaagac
    10201 acgacggcgt tggggactga gttacggcgc agccgctgct cgtggcgggt catccgccag
    10261 tccgccgcct tgaggacgag gaatgccgtg ccgatgccgc ccaggtgtgc gagatgcgac
    10321 acgccgtcgc tcagaccggt ggcggcgctg agccacgcga gtgccagcga gatcgccacc
    10381 gtgaaggtca ccagccactt ggcggcgaac ggctccggga acgggaagac ctgcactggg
    10441 gcgtcgggcc agtaccaggc gaaggccacc gccacgccga ggaccgcgcc ggacgcaccg
    10501 acgacgagct gatccccgaa cagcggcacg agcagcgcgg agagcaccgc tcccccgatg
    10561 ccacacagga tgtagaaccc gatgaagcgc cggccgccga ggcgagcctc caccgggggt
    10621 ccgaagaagt agagcgcgac caggttgaag gcgagatgca gaatgctcgc gtggacgaac
    10681 tgataggtga ccagggtcca cgggtgcgcc cacaccgcga gcggcgagaa gccgaacagg
    10741 gcgagaaagc gcggcgccca gaacagcgtg ctcttgagga ggtacgcgat caggttggcg
    10801 accaacaggc gcccgaccca cggggtcacc ccgtcggtgc gttccgggat catggtcatt
    10861 attcccggcg ccccacgcga aagttccgtg ccaggcggca gatacgctca cacatatccg
    10921 gatactcgat cccggctgcc cgggcagcct ggggaaacag gctcgtgcca gtcatcccgg
    10981 ggagattgtt cgcctcgaga cagaagatgt ccccctcggc ccccaggcgg aagtcgatgc
    11041 gcgagtaacc ccccggtttc agcgcccggt gcgccaccac ggccagcgcc tgcacccggc
    11101 gggcgatcgg cggttccagg gcggcgggga agatctcttc ggacatgccg ggcgtgtact
    11161 tgcactcgta gtcgaacaac tcgtgacgcg ggacgatctc cccgacggga agggcctcgt
    11221 cacccagcac gccgaccgtg agctcccggc cggggatgaa ctgctcgatc atcacctcat
    11281 cgtcccagcg gacggcctcc gccacggccg tggcgagacc ggccttccgc ttcaccaggg
    11341 tcaacccgac ggtggatccc tgcttcgacg gcttcacgat caccggccat cccagctctg
    11401 cggccgcgtc cgccggctcg gccgggggcg tcatgcgcca ctccgggacc ggaacccccg
    11461 cgtcccggaa cagccgcttg gacaggtcct tgtccacggc gagggcggac ccgagcgggc
    11521 ccgagccggt gtacgggacg ccgatcatct cgagcaccgc ctggagcagg cccccttcgc
    11581 ccgctccacc gtgcaccacg ggaaacacga cgtcggctcc ctgcacgacg gcgagtccgg
    11641 cgaggccgcc gcacagcatg cgacgctcgt ccgctatcag ctcggaagtg tcggggagca
    11701 ccgttcccac gtggggcacc agcaaccggg cctccgctgc ggcggtgagg acgccgctcg
    11761 cgatgtcgac cacgtggacc tggtgtccac ggccccgcag ggcggccacc acctgcgcgg
    11821 ccccggcgaa ggcgacagtc cgctccgcgg tggcgccacc cgtcagaacc gtgatccgca
    11881 tctaccggat gccggcgagc tgatgcagca ggtcgtaccg ggtgacgatc ccggtcagtt
    11941 cgcggtcgcg ccgaaccagc actgccggcg tctcgcgcga caacagcgcg gtgagccggt
    12001 cgagcggcca gtccacgtcg accaccggaa aggggggatc catcagatcg cgcaccgcct
    12061 ggtccagaac cgc
//
LOCUS       DBNGZW010000662         6788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_61803, whole genome shotgun sequence.
ACCESSION   DBNGZW010000662 DBNGZW010000000
VERSION     DBNGZW010000662.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6788
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_61803"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            417..914
                     /locus_tag="AC16JC_15585"
     CDS             417..914
                     /locus_tag="AC16JC_15585"
                     /inference="COORDINATES: protein motif:HMM:NF025710.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HIR5166171.1"
                     /translation="MKRRLMQPLALLAMVAGLAPLATAQSNSGSITATAVVQQPISVI
                     GAVNLSFGNVFPGVPATVALTDAGAGRFDVAGQANAPVTMSFTALPGNLTFGANNMPV
                     VFTAGFNTTNAAAGATPFTPASGASTSLSGTGALFVFVGGTVTPAVNQAAGTYTGTVT
                     LQVTY"
     gene            936..1460
                     /locus_tag="AC16JC_15590"
     CDS             936..1460
                     /locus_tag="AC16JC_15590"
                     /inference="COORDINATES: protein motif:HMM:NF025710.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4402 domain-containing protein"
                     /protein_id="HIR5166172.1"
                     /translation="MVSRAGLPGLCRRVRWWSGVPALLAVAALPAPAAPVLAQGQPVR
                     VNGVRSVSFGTVFPGISKSVLRTDALNSGRFDIRAAAGTQLQLSFALPSAMTGPGGAT
                     MPLTFGGGDAGFSATQTVASQVGFDPRVPFVATMSGPPARAAVFLGGTVTPLASQPAG
                     SYTGTITLTVVYFP"
     gene            1457..2287
                     /locus_tag="AC16JC_15595"
     CDS             1457..2287
                     /locus_tag="AC16JC_15595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0043711 - pilus organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis chaperone"
                     /protein_id="HIR5166173.1"
                     /translation="MTRTAWLTLAALCATTRLSAQAVMVAPKAVFMSSGGGSGTVTLL
                     NPGTDPVEVAVSVFYGYTATDSAGQLRLVTIDHPDSTQPSAAAWLQAFPRRLTLGPRD
                     QQVVRLLATPPAGLPAGEYWARLAFTAKAGELPVTGVSDSANIKVGLTLQIQQVIGLH
                     YRNGQVRTGITVSGLDAVRQGDSVIVHAGLERQGNAAYLGTVHAVVVGSNGKPVGELR
                     VPITVFFANRPRWAVSIAGAPADQYQLRLDITTRREDLERRVLLQSPAVSDSTVVKSP
                     "
     gene            2284..4968
                     /locus_tag="AC16JC_15600"
     CDS             2284..4968
                     /locus_tag="AC16JC_15600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166174.1"
                     /translation="MISRAAALALLGPLLGLGAVAAPTSVRAQARVVSRPAIPERSLP
                     DTEKPFSAGSQDSVPQPVLVELRIRGLASATVRAYRVRTEALVPLAQFLSLAQVRYHL
                     SPDGVLDASVPPGPSRLVVAAGNDTMTFGDHRVRLEPDFRLFKDGELYVGAERLGNLL
                     GSAFVVDWAGLTVTLLDAGMLPVGQRARREAARAALARRYEAAPADVQLGLERQRWDG
                     LVLDYSLFAPAGRPVGAGSYAFDLGGDAFGGSLAVGARSVGPADAGVARGDVSWTGVW
                     EERAWLKQIRLGDGFSTGPRLRELRGVALTNAPFLRPALLGTSRYLGRLEPGWSVEAY
                     QNGQLVAVDTTDVVGAYGFALPVRYGENPVDFVAYGPLGEVREFDRTYRVLGELLPFR
                     RFEYGVSGGACRDPRCRASGNVDLRYGLTPRVTFQGGADRFWRNALADLWHPYASVTA
                     NPTNDWALELSGVGNALVRGAVQFEPSIDLRVSAAAAAFDTRPDAPIVTVAGQQSAWT
                     VSALVRPLPKYGLFFFEGNIDQVRARSATTTRARLGASVQTGPTRLMPYVRVERQALD
                     GAGGRTDPFVGVSAFVLPHPRWGPVLGPAFFRGALESRVWGGGSAPRLTQASLFASRP
                     LWGGVSLETGAVWQRGSRGPLIQVTVNTYLSAMRSYTTMSAAAGSPAMVSQLVQGSVL
                     WDRAGGRLATAPGPSIERAGIAGRVFLDRNQNGRYDPGEPPVGGVRVIVGPRPAVSDS
                     EGAFRVWDLMPFEPVDVLVDSLSIDSPLLVPAFARATLVPNPNRFRTLDVPIVAAGVI
                     EGHVSREIGGTREPVGVAGLVLTDRRSGTRRRFVSFSDGSFYLMGVKAGDYELAVDAT
                     AGGDVGAGLVAEPLRFTIEPEAAASGRSGLELVLRPGR"
     gene            complement(5000..>6788)
                     /locus_tag="AC16JC_15605"
     CDS             complement(5000..>6788)
                     /locus_tag="AC16JC_15605"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR5166175.1"
                     /translation="SGGVVAAATPAGPRAATPDSALVAPRLLAVLLGFAGLVALHIRS
                     MASGSMGVEDVLVPAVGGVIIAARVAYSLMADRRYEAALEREVERQTQTLVDSLTTTA
                     SAERNLRQIMEASPDPIVLLNRDGQVLDYAPDRPAHGAAAGSRTAFDFVDPDAAALLR
                     AHLEAAFAGEVRRFEVPQRRPDGARVVSSVVYAPIREHGQITRVLAISRDVTELRRAQ
                     SQLQQADKLAAMGQLVSGVAHEINNPAAIISGFAQTLMLDDLRPEQREIVEMVRDEAM
                     RIGQITTNLLAFARMSGPERSLVDVNELVRRTHTLRSYHLNTLNVEVRLDLDPVEPRV
                     WANMSELQQMLLNFLINAEQALDGFPGHRGITLRTHAEGPEVKFECRDTGPGIAPEIR
                     SRIFDPFFTTKPEGVGTGLGLSICYGIVRSHGGRISVDSEPGRGASFLVGLPLDLRTM
                     PRPSGGAPVQGPAAAEQPVSVLVVDDEPGIRQATARFLNRSGMEVRAVSDGAEALRAL
                     RGQSFDVILCDVRMPGMNGRDFLIRLREQAPELVSTLIFATGDSLDADTAALLADSGA
                     PSLVKPFDFDALERLVRVVAARPSRRAGT"
BASE COUNT          916 a         2351 c         2416 g         1105 t
ORIGIN      
        1 ggtctgcccc aggcgcccgg attcgcgcta tcggtcccca tgcaccacgc cgtctccctg
       61 ctgccgcccg atgtgaagca gcggctcggc atcccgtagg gctacctgtt gcagagtcgg
      121 ccacttagct gcattccgcc ataatctata aaacatgcaa tatcgtcata ccgggctcgg
      181 tgtgacgcgt gacccactcg ggtgctcgac tcccgccctt gcatgcaagt catttttcta
      241 ttgtcgttta gatgcctagc gtgtattatg ggcggtctcc aggtgtcaaa aaatatgacg
      301 gtttgtagga aaccgcttac ttcgtaggaa acggcttaca gggaggcacg gggcttgcct
      361 cggtcagaca caggaattcg agcaggctcc gcaacctcaa ggggagagca ctgacgatga
      421 agcgcaggtt gatgcagccg ctggcattgc tggccatggt cgccggtttg gcgccgcttg
      481 ccacggcaca gtcgaactcc gggagcatca cggcgacggc cgtggtgcag cagccgatca
      541 gcgtgatcgg tgcggtcaac ctgtcgttcg gcaacgtgtt cccgggtgtc cccgcgaccg
      601 tcgcgctgac cgacgccggc gccggccggt tcgacgtggc cggtcaggcg aacgcaccgg
      661 tgaccatgtc gttcaccgcg ttgccgggga atctcacctt cggcgccaac aacatgccgg
      721 tggtgttcac cgctggtttc aacacgacga acgcggccgc cggggccacg ccgttcacgc
      781 cggcgagcgg ggcgtcgacc agtctgtccg ggacgggcgc cctgttcgtc ttcgtgggcg
      841 gcacggtgac gccggcggtg aaccaggccg cgggcaccta caccggcacc gtcaccctgc
      901 aggtgacgta ctgacctaac cggattcacg gccgcatggt gagccgtgct ggcttgccgg
      961 gcctctgccg gcgcgtgcgt tggtggagtg gcgtaccggc gttgctcgcc gtagcggcac
     1021 tccccgcacc ggcggccccc gtgctcgctc aggggcagcc ggttcgggtc aatggcgtgc
     1081 ggtcggtcag tttcgggacg gtatttccgg gcatctccaa atccgtgcta cgcaccgatg
     1141 cactgaacag cgggcggttc gatattcgtg cggcagccgg cactcaactc cagctgagtt
     1201 tcgcactgcc ctcggcaatg acggggccag ggggcgcgac gatgccgttg accttcggcg
     1261 ggggggacgc cggcttctcc gccacgcaaa cggtggcgag ccaggtcggg ttcgatccac
     1321 gcgtcccctt cgtggcgacg atgagtggtc cgcccgcccg ggcggcggtg tttctcggcg
     1381 gcactgtcac gccactcgca tcgcagcccg ctggtagcta tacggggacg atcaccctca
     1441 ccgttgtgta cttcccatga cccgaaccgc gtggctcacg ctggccgcgc tgtgcgccac
     1501 tacccgattg tcggcgcagg cggtcatggt cgcccccaag gcggtgttca tgtcatcggg
     1561 cggcggcagc gggaccgtca ctctgctcaa ccccgggacc gatccggtcg aagtggcggt
     1621 ctccgtcttc tatggctaca cggcgacgga ttccgccggc cagctgcggc tggtcacgat
     1681 cgaccatccc gattccacgc aaccttcggc ggcggcatgg ctccaggcgt tcccccgccg
     1741 cctgacgctg gggccgcggg atcaacaggt cgtgcggctg cttgccacac cgccggcggg
     1801 ccttcccgcc ggcgagtact gggctcgact ggccttcacc gccaaggcgg gagagctgcc
     1861 cgtcaccggt gtgagcgaca gcgccaacat caaggtcggc ctcacgctgc aaatccagca
     1921 ggtgattggc ctccactatc gcaacgggca ggtgcgaacg gggatcacgg tgtccggact
     1981 ggacgcggtg cggcagggcg attcggtgat cgtgcacgcg gggctcgagc gccaggggaa
     2041 cgccgcctac ctcggtacgg tgcacgccgt cgtcgtcggt tcgaacggga aacccgtggg
     2101 cgaacttcgc gtgccgatca cggtgttctt cgccaatcga ccccgctggg ccgtgagcat
     2161 cgccggcgca ccggccgatc agtatcagct gcggctcgac atcaccaccc ggcgggagga
     2221 cttggagcgt cgggtcctgc tgcagagccc ggcggtgagc gattcgacgg tggtcaagag
     2281 tccgtgatct cgcgcgccgc ggctctggcc ctcctgggcc cgctgctcgg cctcggcgcc
     2341 gtcgccgccc ccacgtccgt ccgggctcag gcgcgggtcg tctcgcggcc ggcaatcccc
     2401 gagcgcagcc tcccggacac cgagaagccg ttcagtgcgg gctcccagga ttcggtgccc
     2461 cagcctgtcc tggtcgagct gcggatccgg ggcctggcct cggcgacggt gcgggcgtac
     2521 cgcgtacgga ccgaggccct ggtgccgctc gcccagttcc tgagtctcgc ccaggtgcgc
     2581 taccaccttt ctcccgacgg cgtgctcgac gcgtcggtcc ctcccgggcc gagccgcctc
     2641 gtcgtcgccg ccgggaacga cacgatgacc ttcggcgacc atcgcgtgcg tctcgagccg
     2701 gacttccgcc tcttcaagga cggcgagctg tacgtcgggg ccgagcggct ggggaacctg
     2761 ctcggcagcg ccttcgtggt ggactgggcc ggcctcaccg tgacgctctt ggatgccggc
     2821 atgctgccgg tcggtcagcg ggcccggcgc gaggcggccc gcgccgcgtt ggcacgccgc
     2881 tacgaagccg cgcccgccga cgtgcagctc ggcctcgagc ggcagcgctg ggatgggctg
     2941 gtgctcgact attcgctgtt cgcgccggcc ggccggccgg tcggggcggg atcctacgcg
     3001 ttcgatctgg ggggtgacgc gttcggcggc tcgctcgcgg tcggcgcgcg cagcgtcggt
     3061 ccggcggacg cgggcgtggc gcggggcgac gtgtcgtgga ccggcgtgtg ggaagagcgc
     3121 gcgtggctga agcagatccg tctgggcgac ggcttctcga ccgggccgcg cctgcgcgag
     3181 ttgcgtggcg tggccctcac caacgcgccg tttctgcggc ccgcgcttct gggtacgagc
     3241 cggtacctgg gccggctcga gccgggttgg tccgtcgaag cctatcagaa cggccagctc
     3301 gtcgccgtcg acacgaccga cgtcgtcggg gcgtatggct tcgcgctccc ggttcgctac
     3361 ggggagaacc cggtggattt cgtggcgtac ggtccgctgg gggaagtccg ggaattcgat
     3421 cgcacctacc gggtgctggg tgagctgctg ccgttccgcc ggttcgagta cggcgtgtcg
     3481 ggcggcgcgt gccgcgatcc ccgctgccgc gcgagcggca acgtggacct gcggtacggt
     3541 ctgacgccgc gagtaacgtt ccagggcggc gccgatcgct tctggcgcaa cgccctcgcc
     3601 gacctgtggc atccctacgc gagcgtgacc gcgaacccga ccaacgactg ggcgctcgag
     3661 ctgtcgggcg tcggcaatgc gctggtgcgg ggggcggtgc agttcgagcc gtccatcgat
     3721 ctgcgagtca gtgctgccgc ggcggcgttc gacacgcgcc ccgacgcgcc gatcgtgacc
     3781 gtggcgggtc aacagtcggc ctggaccgtc tcggccctgg tgcggccgct gccgaaatac
     3841 ggattgttct ttttcgaagg caatatcgac caggtacgcg cccggtccgc gaccacgacc
     3901 cgcgcccgcc tcggcgcctc ggtgcagacc gggccgaccc ggttgatgcc ctacgtgcgg
     3961 gtcgagcgcc aggcgctcga cggcgcgggc ggccgcaccg accccttcgt cggcgtgagc
     4021 gccttcgtgc tgccgcaccc gcggtgggga ccggtgcttg gccccgcatt cttccgtggc
     4081 gccctcgagt cccgcgtgtg gggcgggggg agcgcgccgc gcctcacgca ggcgtcactg
     4141 ttcgcctcgc ggccgttgtg gggcggcgtg agcctcgaga cgggggctgt gtggcagcgc
     4201 ggcagccgag ggccgctgat ccaggtgacg gtgaatactt atttgtcggc gatgcgctcc
     4261 tacacgacca tgagcgctgc ggccgggtca ccagccatgg tgtcgcagct ggtgcaggga
     4321 tcggtgttgt gggatcgcgc cggtggccgg ctggcgacgg ctccgggtcc ctcgatcgag
     4381 cgggcgggga tcgcgggccg tgtcttcctc gaccggaacc agaacggccg gtacgatccg
     4441 ggcgagcccc cggtcggcgg ggtgcgggtc atcgtcggac cgcgaccggc ggtgtcggat
     4501 tccgaggggg cgttccgggt gtgggacctg atgccgttcg agcccgtgga cgtcctggtg
     4561 gattcgctga gcatcgactc cccgctgctc gtgcccgcct tcgcgcgggc cacgctcgtt
     4621 cccaacccga atcggttccg cacgctcgac gtgccgatcg tcgcggccgg cgtgatcgag
     4681 ggtcacgtgt cgcgggagat cggcggcacg cgtgagccgg tgggagtcgc gggcctggtc
     4741 ctcaccgacc ggcggagcgg tacgcgtcgc cggttcgtct cgttctccga cggctcgttc
     4801 tacctgatgg gtgtgaaggc gggggactac gagctggcgg tggacgcaac ggctggcggc
     4861 gacgtcgggg cagggctggt cgctgagccg ctgcggttca cgatcgagcc cgaggcggca
     4921 gcttcgggac gcagcggcct cgagctggtg ctccgtcccg gtcgctgagc ccttcccgct
     4981 tcccccttcc cccttcccgt taggtgcccg cccgccgcga cggtcgcgcg gcgacgaccc
     5041 gcaccagccg ctccagcgcg tcgaaatcga acggcttcac gagcgagggc gccccggaat
     5101 cggccagcag cgccgcggtg tcggcatcga gcgagtcacc ggtcgcgaag atgagggtgg
     5161 agacgagctc cggcgcctgc tcgcggagcc ggatcaggaa gtcgcgcccg ttcattcccg
     5221 gcatccggac atcacacagg atcacgtcga agctctgccc acggagcgcg cggagcgcct
     5281 cggcaccgtc gctgacggca cgcacctcca tgccgctgcg gttcaggaag cgggcggtgg
     5341 cctgccggat gcccggctcg tcgtccacta cgagcacgga caccggctgt tccgcagccg
     5401 ccggaccctg gaccggcgcg ccgcccgagg ggcgcggcat ggtgcggaga tcgagcggta
     5461 gccccaccag gaacgacgcc ccgcgtcccg gctccgagtc gaccgaaatg cgcccgccgt
     5521 ggctgcgcac gatgccgtag cagatcgaga gccccagacc cgtgcccacc ccctccggtt
     5581 tggtggtgaa gaacggatcg aagatgcggg agcggatttc cggtgcgatg ccgggaccgg
     5641 tgtcgcggca ctcgaatttg acctcgggcc cctcggcgtg cgtgcggagg gtgatgccgc
     5701 ggtgtccggg gaagccgtcc agcgcctgct cggcgttgat cagaaagttc agcagcatct
     5761 gctgcagctc cgacatgttg gcccagacgc ggggctcgac cggatccaga tccagccgca
     5821 cctcgacgtt caacgtgttc agatgatagg agcgcagggt gtgcgtccgg cgcaccagct
     5881 cgttgacgtc caccagtgac cgctcgggcc cgctcatccg cgcgaaggcg agcaggttgg
     5941 tagtgatctg gccgatgcgc atcgcctcgt cgcgcaccat ttcgacgatt tcgcgctgct
     6001 cgggccggag gtcgtccagc atcagggtct gggcgaagcc ggaaatgatc gcggccggat
     6061 tgttgatctc gtgcgccacg ccgctcacca gctgacccat ggccgcgagc ttgtcggcct
     6121 gctgcaactg gctctgggcg cgccgcagct cggtgacgtc gcgggagatg gccagcaccc
     6181 gggtgatctg cccgtgctcc cggatcgggg catacaccac cgagctcacc acgcgcgccc
     6241 catcgggccg ccgctgcggc acctcgaacc ggcgcacctc ccccgcgaac gccgcctcga
     6301 ggtgggcgcg cagcagcgcg gccgcgtccg ggtccacgaa atcgaacgcc gtgcggctac
     6361 cggcagccgc gccgtgcgcc ggtcggtccg gcgcatagtc caggacctgg ccgtcccggt
     6421 tcagcagcac gatcggatcg ggcgacgcct ccatgatctg gcggaggttg cgctccgcgg
     6481 acgcggtggt cgtcagcgaa tcgacgagcg tctgcgtctg ccgctcgacc tcgcgctcca
     6541 gcgcggcctc gtagcgccgg tcggccatca gggagtacgc gacgcgggcc gcgatgatca
     6601 ccccgccgac ggcggggacc agcacgtcct ccacccccat cgatcccgag gccatgctgc
     6661 ggatgtggag ggccaccagc ccggcgaacc cgagcagcac ggccagcagt cgcggggcga
     6721 cgagggcgct gtccggggtg gccgcccgcg gtcccgccgg agtcgccgcg gccacgaccc
     6781 cgcccgac
//
LOCUS       DBNGZW010000663         2266 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_673436, whole genome shotgun sequence.
ACCESSION   DBNGZW010000663 DBNGZW010000000
VERSION     DBNGZW010000663.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2266)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2266)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2266
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_673436"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /gene="asnS"
                     /locus_tag="AC16JC_15610"
     CDS             <1..929
                     /gene="asnS"
                     /locus_tag="AC16JC_15610"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HIR5166176.1"
                     /translation="SRRQVAIARVRHEVIQAIRDFFYERGFVLVDTPILTGSIGEHAG
                     TLFATEYFDLGKAFLAQTGQLYVEAAAAALGKVYCFGPTFRAEKSKTRRHLTEFWMVE
                     PEVAWNDSDDNMRLQEQFVAYIVGRCLERRRPELAELERDTAPLERCTPPFHRISYTD
                     AVEKLRGLGATMEWGRDLGGDDETLLAKQYDRPVMVYNYPRAVKAFYMKENPADPRTV
                     LNNDMLAPEGYGEIIGGSQREDDYDRLARRIQEEGLPLEAYGWYLDLRKYGTFVHAGF
                     GLGVERTVAWICGIPHIREAIAFPRTLYKLWP"
     gene            940..1236
                     /locus_tag="AC16JC_15615"
     CDS             940..1236
                     /locus_tag="AC16JC_15615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166177.1"
                     /translation="MFLIIWAFTARAGRELEFERVYAPDGDWSRLFARSADYHGSELL
                     RHAAGGGYLTIDRWSSSAAFAVFRSRWQLEYEALDRSCEDLTEREALVGRFETA"
     gene            complement(1245..2018)
                     /gene="mazG"
                     /locus_tag="AC16JC_15620"
     CDS             complement(1245..2018)
                     /gene="mazG"
                     /locus_tag="AC16JC_15620"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HIR5166178.1"
                     /translation="MQGDSALGRVLALVRELRKRSPWNAAQTPYTLRPYLVEEVLELD
                     HAIASGDPLQLREELGDMLLHLSFQIVLAEERSQLDAETVVRTLEQKMRRRHAWLLGG
                     HPSTAATQRDSWERSKRAEAGASGRSAFAGVPPHLPPLLMALRLQERAAGVGFDWPDE
                     KGPQAKVREEMAELEEVSREAADRERMAIELGDLFFAAVNLSRKLGVDPNQALEIANR
                     KFRSRFEAVEHLARERGVEVGEATLDELDTLWEEVKEGT"
     gene            2064..>2266
                     /locus_tag="AC16JC_15625"
     CDS             2064..>2266
                     /locus_tag="AC16JC_15625"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HIR5166179.1"
                     /translation="MSAPRSRAWVEVSLAAVVDNARTVARTAGTRLLPIVKANAYGLG
                     AVPVSRALEAVDPWGYGVATVEEG"
BASE COUNT          337 a          794 c          761 g          374 t
ORIGIN      
        1 gcagccgccg gcaggtcgcg atcgcgcggg tgcgccacga ggtgatccag gcgatccgcg
       61 acttcttcta cgagcgcggc ttcgtgctcg tggacacgcc gatcctcacg ggatcgatcg
      121 gcgagcatgc cggcacgctg ttcgccaccg agtacttcga cctgggcaag gcgtttctgg
      181 cgcaaaccgg ccagctctac gtcgaggccg cggcggccgc cctcggcaaa gtgtattgct
      241 tcggccccac gttccgcgcc gagaagtcca agacccgccg tcatctgacc gagttctgga
      301 tggtcgagcc ggaggtggcc tggaacgact ccgacgacaa tatgcggctg caggagcagt
      361 tcgtcgccta catcgtcggc cgttgcctcg agcgccgtcg cccggagctg gccgagctgg
      421 agcgcgacac tgcccctctg gagcgttgca cgcccccgtt tcaccgcatt tcctacaccg
      481 acgctgtgga aaagctcagg ggcctggggg ccaccatgga gtgggggcgc gacctgggcg
      541 gcgacgacga gactctcctg gccaaacagt acgaccgtcc cgtcatggtc tacaactacc
      601 cccgggcggt caaggccttc tatatgaagg aaaacccggc cgacccacgg accgtgctca
      661 acaacgacat gctggcgccg gaggggtacg gcgagatcat cggcggcagc cagcgcgagg
      721 acgattacga ccggctggcc cgccgcatcc aagaggaagg actgccgctc gaggcctacg
      781 gctggtacct cgatctccgc aagtacggca cgttcgtcca cgccgggttc ggcctgggtg
      841 tggagcggac ggtcgcctgg atctgcggca tcccgcacat ccgtgaggca atcgcgtttc
      901 cgcgcaccct ctacaagctg tggccgtgaa gccgtcgccg tgttcctgat catctgggcg
      961 ttcaccgccc gggccgggcg cgagctcgag ttcgagcgcg tgtacgcgcc ggacggggac
     1021 tggtcccggc tgttcgcgcg cagcgccgac tatcacggct ccgagctgct gcgccacgcg
     1081 gcgggcggcg ggtacctgac gatcgaccgc tggagctcca gcgcggcgtt cgctgtgttc
     1141 cggagccggt ggcagctgga atacgaagcg ctggatcgct cctgcgagga tctcaccgag
     1201 cgcgaggcgc tcgtcggccg gttcgagacc gcctgaggca gggctcaggt tccctctttc
     1261 acttcctccc acagcgtgtc cagctcgtcc agcgtggcct ctcccacttc cacgccgcgc
     1321 tcccgcgcga ggtgttccac ggcctcgaag cgcgagcgga acttccggtt ggcgatctcc
     1381 agcgcctggt tcgggtcgac gccgagtttg cgcgacaggt tcaccgccgc gaagaacagg
     1441 tcgcccaatt caatggccat gcgttcccgg tcggcggcct cccgcgatac ttcctccagc
     1501 tcggccatct cttcgcgcac cttggcctgc ggtccctttt cgtcgggcca atcgaagccc
     1561 acgcccgcgg cccgctcctg caagcgcaac gccatgagca gcggcggcag gtgcggcggc
     1621 acgccggcga acgcgctgcg gccgctcgcc cccgcttccg cccgcttgct gcgttcccag
     1681 gagtcgcgct gggtcgccgc cgtggagggg tggccgccga gcagccacgc atggcgccgc
     1741 cgcatcttct gctccagcgt acgcaccacc gtctcggcgt ccagttggct gcgctcttcg
     1801 gcgagcacga tctggaacga caggtgcagc agcatgtcgc ccagctcctc gcgcagctgc
     1861 agcggatcgc cgctcgcgat ggcatgatcg agctccagca cttcctcgac caggtacggg
     1921 cggagtgtgt agggggtctg ggcggcgttc cacggcgagc gcttccgcag ctcccgcacg
     1981 agcgccagca cacgtccaag cgctgaatcg ccttgcattt agcctccggg cggggtatga
     2041 tacccgctcc tctgatggag tcaatgagtg cgccgcgcag ccgcgcgtgg gtcgaggtaa
     2101 gcctcgcggc tgtggtcgac aacgcccgca cggtggcgcg cacggcgggc acgcggctcc
     2161 tcccgatcgt gaaggccaac gcgtacgggc tcggagccgt tcctgtctcc agggcgctgg
     2221 aagcggtcga tccctggggg tacggcgtcg ccacggtgga ggaggg
//
LOCUS       DBNGZW010000664         8382 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_879516, whole genome shotgun sequence.
ACCESSION   DBNGZW010000664 DBNGZW010000000
VERSION     DBNGZW010000664.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8382)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8382)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8382
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_879516"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1283)
                     /locus_tag="AC16JC_15630"
     CDS             complement(<1..1283)
                     /locus_tag="AC16JC_15630"
                     /inference="COORDINATES: protein motif:HMM:NF025092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HIR5166180.1"
                     /translation="MAPSASLRRALTLALLLGSPAAAQTGGAIAGRVRDAASGQGIGG
                     ATISVDGGRQGASTDTAGFYRVREVRSGWHRVQVARIGYRPMVFDSVLVRSGETVTLH
                     VSMETLALEIESIAVTAVPDRVLDPMAPQDLQRVTGEEIRRLPVTTVEEAVALSAGAV
                     GESYRGGRLGEQAFVLDGIGLKNQLDASTGSLGIRIPPDILTEASLVTNGFSARYGQA
                     ISGLVNVVTKDAGDRWEGRAAYESDRPLPRGWDYGIDRLVLSGAGPLTRGIGLAGVLD
                     VQGRLDADPVNAPPPPDPRDPRNQNPSLLPHNSGERYDATLKFTIPVGARQTLRLFGL
                     HSVEQRLLFDAAYKYDPVLAPAQRVRGTLLTGQLQHTSAPEARTPLTADLRVGYFDRL
                     FLRGGVSDQPAQHFGAFTFETLHFLGEDLARRQDTA"
     gene            complement(1262..2299)
                     /locus_tag="AC16JC_15635"
     CDS             complement(1262..2299)
                     /locus_tag="AC16JC_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5166181.1"
                     /translation="MTPGPYTPHGPLGTGSVVRLTFNLGADLDPAWLPDGTGFIYTRE
                     RPDRPDRDRCLAQMPATGGSIAREICNQSAASADSVDAFSAAAATGSGQMAYVRASAP
                     IEFTWPLAPRFHDLVLATLAEPGRFSAAESFPLLAPSGRTHDEAAQLHWLNDHSLVYL
                     AQSVRYMPPCAFCGPDTISIGLEIARIDFGGSAPAVSILPGTDQASSLTTVGSDTVYF
                     TVYDDSRVFRLALGSGALDVAHDFGVGAIARDVQVSAGRLVAVVGGSVEFRVDSVLGP
                     IARDRGGDVHVVDLQSGADQPRAAAGLLFRHLALDPSGRVVIAEGRPIVINGADTTFL
                     PAGDLWRLQLP"
     gene            complement(2439..3254)
                     /gene="speB"
                     /locus_tag="AC16JC_15640"
     CDS             complement(2439..3254)
                     /gene="speB"
                     /locus_tag="AC16JC_15640"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HIR5166182.1"
                     /translation="MSGPGVPTLLGLPFDAASSFRRGSAAAPAAIRAALTSPGGNPWT
                     EGLVDIGAPETLADAGDLPLPATHSPEARAAIEAGVTRLLAGGGRPIVLGGDHSITYP
                     VLRAMRPRHPRLSVLHVDAHPDLYEEFDGDRYSHACPFARALEERLLDELIQVGIRAT
                     SASQRAQAERYGVQVIDMDAWVAGRRPAPAHPVYLSIDLDGLDPAHVPGVSHPEPGGL
                     TVREVLTLLHRLEVPIVGADVVELNPELDALGLTARVAAKLVKEIAGRMVSSG"
     gene            3310..4335
                     /locus_tag="AC16JC_15645"
     CDS             3310..4335
                     /locus_tag="AC16JC_15645"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013919340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR5166183.1"
                     /translation="MRITELHPAADHELVLMGEEERAGYRAIIAIHSSRLGPAVGGTR
                     LWRYGSTDEALTDALRLSRGMSYKNAVAGLPMGGGKSVILANGAPVSRERLFTAHGRF
                     VESLGGRYITAEDVGTSHRDMAIVKRETRHVAGLRDPSPVTARGVFRALLAAARHRWG
                     AADLAGRTVALQGCGNVGSALARALVDAGVRLVVSDVDPGAGRRCAAETGARSVAPDA
                     IYEVPADVFAPCALGGILNDRTIPRLRCEVVVGAANNQLAVPADADRLAARDILYVPD
                     YVANAGGVIDGARDICGWSDVRVSTAVEGIFDTTRDILERAKTEGVTPAAAADRRAEA
                     ALRRPGS"
     gene            4332..6602
                     /gene="pbpC"
                     /locus_tag="AC16JC_15650"
     CDS             4332..6602
                     /gene="pbpC"
                     /locus_tag="AC16JC_15650"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HIR5166184.1"
                     /translation="MSRRLRLLLAAAFSLALIAALVVWVALPLPTSLASPGPVPRLVL
                     LDRHGLPLRATRSDEGSRGGWTPLAELDPRILQAFLAVEDRRFYGHHGVDPRALGRAL
                     RDDLRARAAVSGGSTITMQLVRLLRGTPRSLAGKLSQILWALRLDAHLDKQTILEQYL
                     NRVPLGQGTVGVTAAAELYFGADPRSVSLGQAALLAALAHAPARDNPLAEPQRARARR
                     AAALARMQALGYARAEDVARARVEPVLRRDAGAPFLAPHFTTRVLQWAEHDGGGAAPS
                     GEWRTTLDLGLQTALEAEVRHTVDVLRDKGVAHAAVVVLDNPSGDILAWVGSPDFFAD
                     TSGQVDMVVSPRQPGSALKPFLYGLAFDRGVTPATVLPDVPRTYQTTLGPYRPRNYDR
                     QFHGPVRARDALGSSYNVPAVELAERLGVSSLLNTLHDAGFASLDHSAEYYGLGLSLG
                     NGDVTLLELANGYRALANGGVWRPVAWRADRETTVGEATARERRVMSRQAAALVLDIL
                     SDPVARIPSFGTETVLDFPFSAAAKTGTSRHFTDNWSVVTTAGFTVAVWVGNFSGRPM
                     QGVGGITGAGPLLHRAALLVAQRYPPGVLPSPAQRGGVPVRICRLSGLLAGPHCPPMT
                     EWFVPGTGPTRPCDWHRNGRVALPAEYAEWAGTQSASPVSAAVASSEPEGFRILSPQD
                     GDRYTIPAGSDPRYATIALRAAGGGDDGARVRWLVDGREWREARWRLVPGRHTVRAVN
                     ARGESDIVTIDVLGNR"
     gene            complement(6664..>8382)
                     /locus_tag="AC16JC_15655"
     CDS             complement(6664..>8382)
                     /locus_tag="AC16JC_15655"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HIR5166185.1"
                     /translation="GPQIWVLGMTGGEARKVTALDDGVGELYWKPDGRGFLAVVDVKW
                     PAEQEIDRRNGEYPTEARLWTELMWRHWVEFRAGHRQHLFAVELATGKATDLTTVDHD
                     VPTIATGGDGDVAVSPDGREIAVAMHGDDVVATNTNVDVYLIGDEGRGTGDESKWKNV
                     TAANKGADNTPRYSPDGKWLSYLSMERPNFEADRQRLMLVPRAGGGAPVEATAGWSLS
                     VGSYTWCPDSKCVYAVVEERGRVNIYRIDLPGFRRTALVAGAGMNTGPSVSADGKTLV
                     YLHQSNTQPPEVWMSGKALTHHTDAAVASLDLRPLEQFGFVGARGDSVFGWLLKPPGF
                     DPAKKYPVIDLFHGGPQTAWNDYWGARWNYEMFASRGYVVVMVNRHGSTGFGQAFTDA
                     VSQHWGDWPYEDLMKGLDVVNQLPYVDSTRECAAGASYGGYMIYWVAGHTDRFKCLVS
                     HDGVFNTMSMGGSTEELWFPNWEFGGTAYQARDQWEKWSPLNYVKNWKTPLLVVHSQL
                     DYRVDLSEGYQAFTAARSMGVPAKFLYFPDEGHWVLRPRNRRIWWGVLLDWIDQYLKA
                     PVVGSR"
BASE COUNT         1244 a         3042 c         2885 g         1211 t
ORIGIN      
        1 gccgtgtcct gccgccgcgc caggtcctcg cccagaaaat gaagcgtctc gaacgtgaat
       61 gcgccgaagt gctgcgccgg ctgatccgac accccgcccc gcaggaacag ccggtcgaaa
      121 taccccacgc gcagatccgc cgtgagcggg gtgcgcgctt cgggggccga cgtgtgctgc
      181 agctgtccgg tgagcagcgt gccgcgcacc cgctgcgccg gcgcgagcac cgggtcgtac
      241 ttgtacgccg catcgaacaa cagacgctgc tcgaccgagt ggagaccgaa gagccgcagc
      301 gtttgccgcg cacccaccgg gatcgtgaac ttgagcgtgg catcgtagcg ctcgcccgag
      361 ttgtggggca ggagcgacgg attctggttg cgcggatcgc ggggatcggg cggcggcggc
      421 gcgttgaccg gatcggcgtc gagccgcccc tgcacatcca gcacgccggc cagtccgatg
      481 ccgcgcgtga gcggccccgc accgctcagc acgagccggt cgatgccgta gtcccatcct
      541 cgtggcagcg gccggtcgct ctcgtaggcg gcccgccctt cccagcgatc gccggcatcc
      601 ttcgtcacga cgttgaccag tcccgaaatg gcctgcccgt agcgcgcgga gaacccgttg
      661 gtgacgagcg acgcctccgt gagaatgtcg ggtggaatcc ggatgcccag cgacccggtc
      721 gatgcgtcga gctggttctt caggccgatc ccgtcaagca cgaaggcctg ctcgcccagg
      781 cggccgcccc ggtagctctc gcccaccgcc ccggccgaca gcgccaccgc ctcctcgacg
      841 gtcgtgaccg gcagccggcg gatttcctca ccggtgacgc gctgcaggtc ctgcggcgcc
      901 atcggatcga gtacccggtc cggcacggcc gtcaccgcga tcgactcgat ctccaaggcg
      961 agcgtttcca tggagacgtg cagcgtcacc gtctcgccgc tgcggaccag cacgctgtcg
     1021 aagaccatcg ggcggtatcc gatgcgggcg acttgcaccc ggtgccaccc gctgcggacc
     1081 tcgcgcaccc ggtagaatcc ggccgtatcg gtggaggcgc cctgccgccc cccgtccacc
     1141 gagatcgtgg cgccgccgat cccctgcccc gatgcggcat cgcgcacccg gccggcgatc
     1201 gccccaccgg tctgtgccgc cgcgggcgag ccgagcagca gggcgagcgt gagagcgcgt
     1261 ctcagggaag ctgaaggcgc cacagatccc cggcgggaag gaaggtggtg tccgccccgt
     1321 tgatcacaat gggccgccct tcggcgatca ccacgcgacc ggagggatca agcgccaagt
     1381 ggcggaacag caggccggcc gccgctcggg gctgatcggc gccgctctgc aggtccacga
     1441 catgcacgtc gccgccccga tcgcgcgcga tgggccccag cacggagtcc acccggaact
     1501 ccacgctgcc tcccaccact gccacgagac ggcctgccga cacctgtacg tcgcgagcga
     1561 tggcgccgac accgaaatcg tgcgcgacgt cgagcgcccc gcttcccagt gcgagacgga
     1621 acacccggga atcgtcgtac acggtgaaat agaccgtgtc gctcccgacc gtggtcaggg
     1681 aggacgcctg atcggtgccg gggagaatgg agactgcggg cgccgaaccg ccaaagtcga
     1741 ttcgcgcgat ctcgaggccg atggagatgg tatccggacc gcagaacgca cagggtggca
     1801 tatagcgcac gctctgcgcc agatacacga gggagtggtc attcagccag tggagctgcg
     1861 cggcctcatc gtgcgtgcgc ccgctgggtg cgaggagtgg aaacgattcg gccgcgctga
     1921 agcgccctgg ctcggccagg gtggccagca cgaggtcgtg aaaccgcggc gccagcggcc
     1981 aagtgaattc aatgggagcg ctggcacgga catatgccat ctgtccgctg ccggtcgccg
     2041 ccgctgccga aaacgcgtcc accgaatcgg ccgatgccgc cgactggttg cagatctccc
     2101 gcgcgatcga cccaccggtc gcgggcatct gcgcgaggca ccggtcgcgg tcaggccggt
     2161 cgggtcgctc ccttgtatag atgaagccgg taccgtccgg caaccaggcc gggtcgagat
     2221 cggccccgag gttgaacgtg aggcggacca ccgacccggt gcccaacggg ccatgtggag
     2281 tgtaaggccc gggcgtcaag ggcccttcgt gaccgcagga agccacgagg accgccagcg
     2341 cgagtggtcc gtgtcgcgtc atgacaagcg gggcgcccct caccggagcc ttcggcggtg
     2401 gaaaggtgcc ctgcccgtgg tgcggggttg cacaaccatc agccgctcga caccatgcgg
     2461 cccgcgattt ccttgaccag cttcgcggcc acgcgagcgg tgagcccgag cgcgtcgagc
     2521 tccggattca gctccaccac gtcggcgccg acgatcggta cctccagccg atgcagcagc
     2581 gtcaacactt cccgcaccgt cagccctccc ggttcgggat gcgacacgcc gggcacatgc
     2641 gccggatcca gtccgtcgag atcgatcgag agatacaccg gatgggcggg cgccgggcgg
     2701 cgcccggcga cccaggcgtc catgtcgatc acctgcacgc catagcgctc ggcctgggcg
     2761 cgctgcgaag cgctcgtcgc ccggatgccg acctggatca gctcgtccag cagccgctcc
     2821 tccagcgccc gggcgaaggg acaggcgtgc gagtagcggt cgccgtcgaa ctcctcgtac
     2881 aggtcgggat gcgcgtccac gtggagcacc gagagccgtg gatgccgcgg ccgcatggcc
     2941 cgcagcaccg gataggtgat cgaatgatcg ccgcccagca cgatggggcg tcccccaccg
     3001 gcgagcaggc gggtcacgcc cgcctcgatc gcggcccgtg cttcgggaga gtgggtcgcg
     3061 ggcagcggca gatcgcccgc gtcagcgagc gtctctggcg ccccgatgtc caccagccct
     3121 tcggtccagg ggttgccgcc gggcgaggtg agcgcggcgc ggatcgccgc gggcgctgcc
     3181 gcagacccgc gacggaacga agacgcggcg tcgaacggca aaccgagcag ggtggggacg
     3241 ccgggaccgg acatgaccaa agcctaaccg gccgtggcgc gcccgccagc gcgttatctt
     3301 gcccttccga tgcgcatcac ggagctacac ccggctgcgg accacgagct cgtcctgatg
     3361 ggagaggagg agcgcgcggg ataccgggcc atcatcgcga tccacagctc ccgactcggg
     3421 cccgccgtgg gggggacccg gctgtggcgc tacggctcga cggacgaggc gctgaccgat
     3481 gcgctccgac tgtcgcgcgg catgtcgtac aagaacgccg tggctgggtt gccgatgggc
     3541 ggcgggaagt cggtgatcct ggcgaacggc gctcccgtct cgcgggagcg gctcttcacc
     3601 gcacatggcc gcttcgtcga atcgctgggc ggccgctaca tcaccgccga ggacgtcggt
     3661 acctcgcacc gggacatggc catcgtgaag cgggagacac ggcacgtcgc cggcctgcgc
     3721 gacccttcgc cggtcacggc gcgcggcgtg ttccgggcgc tcctcgccgc ggcacgccac
     3781 cgctggggcg cagcggatct cgccgggcgg accgtcgcgt tgcagggatg cggcaatgtg
     3841 ggcagcgcgc tcgcccgcgc gctcgtcgac gccggcgtcc ggctggtcgt ttccgacgtg
     3901 gacccggggg ccgggcgccg ctgcgcggcg gaaaccgggg cgaggagcgt ggctcccgat
     3961 gcgatctacg aggtccccgc cgacgtcttc gccccatgcg ccctgggcgg catcctgaac
     4021 gaccgcacca tcccccgcct gcgctgcgag gtcgtggtcg gcgccgcgaa caaccagctc
     4081 gccgtgccgg cggacgccga ccggctcgcg gcgcgcgaca tactctatgt ccccgattac
     4141 gtcgccaacg cgggtggggt gatcgatggg gcgcgcgata tctgcggctg gagcgacgtg
     4201 cgcgtgagta ccgccgtcga ggggatcttc gataccacgc gcgacattct ggagcgtgcc
     4261 aagacggagg gcgtcacgcc ggccgcggcc gcggatcggc gggccgaagc cgctctccgg
     4321 aggcccggct cgtgagtcgt cgcctccggc tgttgctcgc cgccgccttc agtctggcgc
     4381 tcatcgccgc gctcgtcgtc tgggtggccc tgccgctccc gacgtcactc gcttcccccg
     4441 gccccgtccc ccgcctggtg ctgctggacc gtcacggcct cccgctgcgg gccacgcgct
     4501 cggacgaggg cagccgtggc gggtggacgc cgctcgccga gctcgatccc cggattctcc
     4561 aggcgttcct cgccgtcgag gaccggcgct tctacggcca ccacggggtg gacccgcggg
     4621 cgctgggccg cgccctgcgg gacgatctgc gggcacgcgc cgccgtgtcg ggcggctcga
     4681 cgatcacgat gcagctcgtc cgcctgctgc gcggcacgcc ccgctccctg gccggcaagc
     4741 tgagccagat cctctgggcg ctgcgcctcg acgcccacct cgacaagcag accattctcg
     4801 agcaatacct gaaccgcgtc cccctgggac aggggaccgt gggagtcacc gcggcggccg
     4861 agctctactt cggcgccgac ccgcgcagcg tgagcctcgg ccaggcggcg ctgctcgccg
     4921 cgctggcgca cgcgcccgcg cgcgacaatc ccctcgccga accgcaacgc gcccgcgccc
     4981 gccgtgccgc cgcgctcgcc cgcatgcagg cgctcggcta cgcgcgcgcc gaggacgtgg
     5041 cgcgcgcccg ggtcgagccc gtgctgcgcc gcgatgccgg cgcgccgttc ctcgcgccgc
     5101 atttcaccac ccgcgtgctg cagtgggccg agcacgacgg cggtggcgcg gcgccgagcg
     5161 gtgagtggcg cacgaccctc gatctgggat tgcagacggc actcgaagcc gaggtgcgcc
     5221 acaccgtgga cgtgctgcgg gacaagggcg tcgcccacgc cgccgtcgtg gtgctcgaca
     5281 acccatcggg cgacatcctc gcgtgggtcg gctcccccga cttcttcgcc gatacgagcg
     5341 gccaggtgga catggtcgtg tccccacgcc agccgggatc ggcgctcaag ccgttcctgt
     5401 acggcctcgc gttcgaccgg ggggtcacgc ccgccacggt gctgcccgac gttccccgca
     5461 cctaccagac caccctcggc ccctaccgcc cccgcaacta cgaccggcag ttccacgggc
     5521 cggtgcgcgc ccgcgatgcc ctcggcagct cctacaacgt gccggccgtc gagctggcgg
     5581 agcggctcgg cgtgtccagc ctgctgaaca cgctgcacga cgccggcttc gcctcgctcg
     5641 accacagcgc ggagtactac ggcctggggc tctcgctcgg caacggcgac gtcaccctgc
     5701 tcgagctggc gaacgggtac cgcgcactgg cgaacggtgg cgtgtggcgg ccggtggcct
     5761 ggcgggcgga tcgtgagacg acggtaggcg aggcgacggc gcgggaacgg cgggtgatgt
     5821 ccagacaggc ggcggcactg gtgctcgaca ttctcagcga tccggtggcg cgcattccga
     5881 gtttcggcac ggagaccgtt ctcgacttcc cgttctcggc ggcggcgaag acggggacga
     5941 gccgtcactt caccgacaac tggtcggtgg tgacgacggc cggattcacg gtcgccgtgt
     6001 gggtcggcaa cttcagcggc cgcccgatgc agggcgtcgg cggcatcacc ggcgccggcc
     6061 cgctgctgca ccgagccgcg ttgctcgtag cccagcgcta tccgccgggc gtcctgccct
     6121 cccccgcgca acgcggtggc gttccggtac ggatctgccg cctgtcgggc ctgctcgcgg
     6181 ggccccattg cccgccgatg accgagtggt tcgtccccgg caccggcccc acgcgcccct
     6241 gtgactggca ccgcaacggg cgggtcgcgc tgccggcgga gtacgccgaa tgggccggca
     6301 cccagagcgc ctccccggtc agcgccgccg tcgcgagcag tgagcccgag ggcttccgga
     6361 ttctctcacc tcaggacggc gaccgctaca cgattccggc cggcagcgac ccgcgctacg
     6421 cgacgatcgc gctgcgtgcg gccgggggcg gcgacgacgg ggctcgcgtc cgctggctcg
     6481 tggatggtcg tgaatggcgc gaggcgcgct ggcggttggt cccgggacga cacacggttc
     6541 gggcggtaaa tgctcgcggg gaaagcgaca tagtcacaat agacgtactc ggtaatcggt
     6601 aatcggcagg ggcgtaccga ttaccgatga ccgatgaccg atgaccgatg accgacgacc
     6661 tgatcaccga cttcccacca ccggcgcttt caagtattga tcgatccagt ccagcagcac
     6721 cccccaccaa atacgacggt tacgcggcct cagcacccag tgaccctcat ccgggaagta
     6781 gaggaacttc gccgggacgc ccatgctgcg cgccgcggtg aacgcctggt agccttccga
     6841 tagatcgacg cggtagtcga gctgggagtg caccaccagc agcggcgtct tccagttctt
     6901 gacgtagttc aacggggacc acttctccca ctggtcccgc gcctggtagg ccgtgccgcc
     6961 gaactcccag ttggggaacc acagctcctc ggtggacccg cccatgctca tggtgttgaa
     7021 cacgccgtcg tgcgacacca ggcacttgaa gcgatcggtg tggcccgcga cccagtagat
     7081 catgtagccg ccatagctcg cgccggcggc gcattcccgc gtcgaatcca cgtagggcag
     7141 ctggttcacc acgtcgaggc ccttcatgag atcctcgtag ggccagtcgc cccagtgctg
     7201 cgacacggcg tcggtgaacg cctggccgaa gccggtcgag ccgtggcgat tcaccatcac
     7261 cactacgtag ccgcgcgagg cgaacatctc gtagttccag cgcgcgcccc agtagtcgtt
     7321 ccaggcggtc tgcgggccgc cgtggaacag gtcgatcacc ggatacttct tcgccgggtc
     7381 gaacccgggc ggcttcagca accacccgaa caccgaatcg ccccgcgccc cgacgaaccc
     7441 gaactgctcg agcgggcgca ggtcgaggga cgcgaccgcg gcgtcggtgt ggtgcgtcag
     7501 cgccttgccg ctcatccaga cttcgggggg ctgggtgttg ctctgatgca gatagacgag
     7561 cgtcttgccg tcggccgaga cggagggacc cgtgttcatg ccggcgcccg cgacgagcgc
     7621 cgtgcgccgg aagccgggca ggtcgatgcg atagatgttc acgcgcccgc gctcctcgac
     7681 gaccgcgtag acgcacttcg aatccggaca ccaggtatag gatccgaccg agaggctcca
     7741 gcccgccgtc gcttcgacgg gcgcgcctcc gcccgcgcgc ggcacgagca tcagccgctg
     7801 gcggtccgcc tcgaagttgg gacgctccat ggaaaggtag gacagccact ttccgtccgg
     7861 ggagtagcgc ggcgtgttgt ccgccccctt gttggccgcc gtgacgttct tccacttgga
     7921 ctcgtccccg gtcccccgtc cctcgtcccc gatgaggtag acgtccacgt tggtgttggt
     7981 ggcgacgacg tcgtccccgt gcatagccac ggcgatctcg cggccatcgg gtgacaccgc
     8041 gacgtcgccg tccccgccgg tcgcgatcgt cgggacgtcg tggtccaccg tggtgagatc
     8101 ggtcgccttc ccggtggcga gctccacggc gaacaggtgc tggcggtgtc cggcgcggaa
     8161 ctcgacccag tgacgccaca tgagctccgt ccacagccgg gcctcggtcg gatactcgcc
     8221 gttgcgccgg tcgatctcct gctcggcggg ccatttcacg tcgaccaccg cgaggaatcc
     8281 acgaccgtcg ggcttccagt acagctctcc cacgccgtcg tcgagcgccg tcaccttccg
     8341 cgcctcgccc cccgtcatgc cgagcaccca gatctgcggg cc
//
LOCUS       DBNGZW010000665         4803 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1751314, whole genome shotgun sequence.
ACCESSION   DBNGZW010000665 DBNGZW010000000
VERSION     DBNGZW010000665.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4803)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4803)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4803
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1751314"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..215)
                     /locus_tag="AC16JC_15660"
     tRNA            complement(144..215)
                     /locus_tag="AC16JC_15660"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(181..183),aa:Gly,seq:ccc)
     gene            complement(339..1934)
                     /locus_tag="AC16JC_15665"
     CDS             complement(339..1934)
                     /locus_tag="AC16JC_15665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5166186.1"
                     /translation="MRKLIGLVIGTLVLASALAGQGWVPAKCDIKPGNYLVNSAVLYL
                     KNAAQTRFDAQKKKDLSDANRSLTQALTSGGQDKNPAAWYYLGRYYVMQNDAYGADSA
                     FDKAEALFPDCKGEIWLFRRNSLWVPAFNAGVAALNAQQYDSAVAAFRRAAVIYDEEP
                     QGLTTLATAFFNMPAETYLPESTFRRMNPAVPDSQFQAAYDAATRTRYDSAAKYFRLG
                     IVAAKDPKFAREKSDAMFNLANAFYAAQRYDSAATGYAEYLKVVPNDAQALARLGDVL
                     QTAGHKDSAMTVYATIIAHADSMDALSLLNAGVSIYNGAPPFPDTVRVSADCRRSRAP
                     GRTPAQRRLIVAQCDSVAADSVKKREATAAQNYSLAAQAFEAGLAHNPKSRDGLFNLA
                     NAYLALKQPDKMLPVAQRLVAIDPMNRNSVRLVAQAWALKSRSDSALHYVTLSDSLLP
                     VDVTVASFAPGEQTTSLSGLVTNYHTTPSAPLTLTFEFLDAGDKVVATQTVDVPAIAA
                     EGSHAFQVQAIGAGIIAWRYTKS"
     gene            complement(1942..3114)
                     /gene="larC"
                     /locus_tag="AC16JC_15670"
     CDS             complement(1942..3114)
                     /gene="larC"
                     /locus_tag="AC16JC_15670"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:7770034 - nickel-pincer nucleotide cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HIR5166187.1"
                     /translation="MIDPCAGISGDMTLGALLSLGVPGSWLAELPGRLGLEGVRVTIR
                     DVRRAAVGCKQVEFSIPEQPHGRHVAELIRLVERAPVSEWVKERAVRAFRAIGEAEGR
                     VHGVAPERVHLHEVGAVDAVLDIVGGIEGFERLGVDAIYHRPVAAGTGWVEAAHGRLP
                     VPAPATALLLEGMELATGGPVEGEATTPTGAALLRALSAGPPPERWRLLSAGWGAGTR
                     DPERYPNALRIFLAEPAMEAGEVSVLATDVDDLSPEYVEPLRQALLAAGALDVQTWPV
                     QMKKGRTGFRCEMVVAESSVAAVTAALFRHSTTAGVRRTRAERVTLPRRQVTVRLSGD
                     VQVRVKVLEGQTGGPPRVKPEYEDVLAAAEALGQAPLEVARAAQRGAEALIANSKE"
     gene            complement(3153..4307)
                     /locus_tag="AC16JC_15675"
     CDS             complement(3153..4307)
                     /locus_tag="AC16JC_15675"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020580251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR5166188.1"
                     /translation="MSRLPWSLECSGCKTVIGADGFPGVCERCGSPFLVRYGTSPGPG
                     LKSGLANRPWTMWRYREWLPLTAGEQPVTLGEGGTPLLEAPRLGARYGFGRLLIKDES
                     VNPTGSFKARGLAAAVTRAVRAGAERFVVPTAGNAGVALAAYAARAHVTARVYAPATT
                     PSTILSQIRSFGADLVLLDGHIGDCGKAARAYAAESGAIDVSTLREPYRIEGKKTLGL
                     ELAEQLGWTVPSAIVYPTGGGTGLIGMWKAFNEMHAAGWITGSLPRFYSVQAAGCAPV
                     VTAFESGAERCTPWPDPHTIAAGLRVPGPLGDRLMLAALRESGGGAIAVSDADLAAAA
                     RELQLVEGIDAAPEGGATLAAARALLARCTIGSGESVVLFNTGAGWLYRT"
     gene            complement(4304..>4803)
                     /locus_tag="AC16JC_15680"
     CDS             complement(4304..>4803)
                     /locus_tag="AC16JC_15680"
                     /inference="COORDINATES: protein motif:HMM:NF020048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR5166189.1"
                     /translation="DDFAAVHEVVTRFLRRRLDEQKALPDLVVVDGGRGQLSAAVAAA
                     NDVGVATPLASLAKREEEVYLPGRAEPLRLSRRSPALQLLQRARDEAHRFAGSYSRTR
                     RAKRTITSELLAIPGVGPGRRRALLERFGSVAGVRTATREEIASLPGFSQRLADRILD
                     RLEQR"
BASE COUNT          763 a         1742 c         1566 g          732 t
ORIGIN      
        1 aaaaacggcc cgtgtcgcca cggaccgtcg gatcgctcgg ggtactgcta ccagttcagc
       61 tgcgcgtacg cccccggcgc cggcccgtgg tgcgggtcgg acgataggag taggcgtacc
      121 agcaaacact cgtgaacatg cagagcgggc gatgggattc gaacccacga cgtccagctt
      181 gggaagctgg cattctaccc ctgaattacg cccgcgaaga ctccgtgccg aaaaatgggc
      241 gattccatct cccccgtcaa cgccgcgcag aacgctgctc gagtcggcgc atcccgactc
      301 gagcaggtca cggcaacccc ggatcacccg aaatacctct agctcttcgt atagcgccag
      361 gcgatgatcc ctgccccgat ggcctgcacc tgaaatgcat ggctgccttc ggcggcaatc
      421 gccggcacgt cgacggtctg cgtggccacc accttgtcgc ctgcgtcgag gaattcgaag
      481 gtcagcgtga gcggcgcgct gggcgtcgtg tggtagttcg taacgagacc gctgaggctc
      541 gtggtctgct cgccgggcgc gaagctcgcg accgtgacat ccaccggcag cagagagtcg
      601 gagagcgtga cgtaatggag ggccgaatcg ctccggcttt tcagcgccca tgcctgtgcc
      661 accagacgca ccgagttccg gttcatgggg tcgatcgcca cgaggcgctg ggcgaccgga
      721 agcatcttat ccggctgctt cagggccaga tacgcattgg cgaggttgaa cagtccgtcg
      781 cggcttttcg ggttgtgcgc cagccccgct tcgaaggctt gtgcggccaa actatagttc
      841 tgtgcggccg tcgcttcccg cttcttcacc gagtcggccg ccaccgagtc gcactgtgca
      901 acaatgagcc gccgctgtgc gggggtccgg cccggtgccc gtgacctacg gcaatctgcg
      961 ctgacgcgca cggtatcagg gaacggcgga gcgccattgt agatcgagac acccgcgttg
     1021 aggaggctga gcgcatccat cgagtcggcg tgggcgatga tggtcgcata gacggtcatg
     1081 gccgagtcct tgtgtccggc ggtctgcagg acgtccccca gtcgcgccag tgcctgcgcg
     1141 tcgttgggaa cgaccttcaa gtactcggca tatcccgtgg cggcggagtc gtaccgctgc
     1201 gccgcgtaga aggcgttggc gagattgaac atcgcgtcgc tcttctcgcg ggcgaacttg
     1261 ggatccttcg ccgcgacgat gcccaggcgg aagtacttgg cggcggaatc gtagcgggtg
     1321 cgcgtcgccg cgtcgtaggc cgcctggaac tgcgagtcgg gcaccgcggg gttcatccgc
     1381 cggaacgtcg actccggcag gtacgtctcg gccggcatgt tgaagaacgc ggtggccagg
     1441 gtcgtgagcc cctgcggttc ttcatcgtag atcaccgccg cccgccggaa tgccgcgaca
     1501 gcggagtcgt attgttgcgc gttcagggcc gccaccccgg cattgaaggc cggcacccag
     1561 agcgagttgc gccggaacaa ccagatttcg cccttgcagt ccggaaagag cgcctcggcc
     1621 ttgtcgaagg ccgaatccgc cccgtaggcg tcgttctgca tcacgtagta gcgcccgagg
     1681 tagtaccagg cagctggatt cttgtcctgc cccccggagg tgagcgcctg cgtgagcgac
     1741 cggttcgcgt cgctcaggtc cttcttcttc tgggcatcga acctggtttg cgcggcgttc
     1801 ttcaggtaca gcaccgcgct gttgacgaga tagttccccg gtttgatgtc gcacttcgcc
     1861 ggcacccacc cctggcccgc cagtgcggac gccaacacga gggtaccgat caccaggccg
     1921 atgagctttc gcatgacgga atcactcctt ggagttcgca atcaaagcct ccgcgccgcg
     1981 ctgtgcggcg cgcgccactt ccagcggtgc ctggccgagc gcctcggcag ccgcgagcac
     2041 atcctcgtac tccggcttca cccggggcgg accgcccgtt tgcccctcga gcaccttcac
     2101 ccgcacctgc acgtccccgc tcaaccggac cgtcacctga cgccgcggca gggtcacccg
     2161 ttcggctcgc gtgcggcgca cgccggccgt cgtgctgtga cggaacaggg cggccgtgac
     2221 cgccgcaacg gacgactccg ccacgaccat ctcacagcga aagccggtcc ggcccttctt
     2281 catttgcacc ggccaggtct ggacgtcgag cgcaccggct gccaggagcg cctggcgcag
     2341 cggctccacg tattcgggac tcaggtcgtc cacgtcggtg gcgaggaccg atacctcgcc
     2401 cgcctccatc gcgggctcgg ccagaaagat gcgcaacgcg ttcgggtagc gctcggggtc
     2461 ccgtgtgcct gcgccccacc cggccgacag cagacgccag cgctccggcg gcggtccggc
     2521 cgagagtgcc cgcaacaggg cggcgccggt cggcgtcgtc gcttctcctt cgaccggacc
     2581 gccggtcgcc agctccatcc cttcgagcag caacgcggtg gcgggtgccg gcacgggaag
     2641 ccgcccatgg gcggcctcca cccagccggt cccggccgcc acgggccgat ggtagatcgc
     2701 gtctacgccg aggcgctcga atccctcgat ccccccgacg atgtcgagca cggcgtcgac
     2761 ggcgccgact tcgtgcaggt gcacgcgctc gggggcgacg ccgtgcaccc ggccctccgc
     2821 ttccccgatg gcgcgaaagg cgcgcacggc ccgctccttc acccattccg acaccggtgc
     2881 gcgctccacc agtcggatca gctcggcaac gtgtcgcccg tgcggctgct cggggatcga
     2941 gaactccacc tgcttgcacc cgacggcggc ccgccgcacg tcacgaatcg tgacgcgcac
     3001 accctccaaa cccaggcgcc ccggcagctc cgcgagccag ctccccggca ctccgagtga
     3061 gagcaatgcc ccgagcgtca tgtcgccgct gatcccggcg caggggtcga tcaccgcgat
     3121 aggcatgtcc gaagtgtcgg aatataaaca ggttaggtcc ggtacagcca cccggcgccg
     3181 gtattgaaga ggaccacgct ctccccgctc ccgatcgtgc agcgcgccag caacgcccgc
     3241 gcggcggcga gcgtggcgcc accttcgggg gcggcgtcga tcccttccac caactggagc
     3301 tcgcgggcgg ccgccgccag atccgcgtcg ctcaccgcga tggcgccgcc cccgctctcg
     3361 cgcagcgccg cgagcatcaa gcgatccccg agtgggccgg gaacccgaag gccggccgca
     3421 atagtgtgcg ggtccggcca gggggtgcag cgctcggcgc cactctcgaa ggccgtcacg
     3481 accggcgcac atccagcggc ctgcacggaa tagaaacgcg gcagcgaacc cgtgatccat
     3541 cccgccgcgt gcatctcgtt gaaggccttc cacatgccga tcaatcccgt gccgcctccc
     3601 gtggggtaca cgatggccga gggaacggtc cagcccagct gctcggcgag ctcgagcccg
     3661 agcgtcttct tcccttcgat gcgatagggc tcgcgcaggg tcgagacgtc gatcgcaccg
     3721 ctctcggccg cgtacgcccg ggccgccttg ccgcaatcgc cgatatggcc atcgagcagt
     3781 acgaggtcgg cgccgaagct gcggatctgc gacaggatcg tggacggagt cgtggccggg
     3841 gcgtacacgc gagccgtcac atgcgcgcgg gccgcgtacg ccgcgagcgc cacgcccgcg
     3901 ttcccggccg tcggcaccac gaaccgctcc gctccggcgc gcaccgcgcg cgtcaccgcg
     3961 gcggccagcc cgcgcgcctt gaacgaaccg gtcgggttca ccgactcatc cttgatcaac
     4021 agccgcccga aaccgtatcg cgcgccaagc cgcggcgcct cgagcagcgg cgtgccccct
     4081 tccccgagcg tcaccggctg ctcaccggcg gtcagcggca gccattcccg gtagcgccac
     4141 atcgtccagg ggcgattggc cagcccggac ttcaggccgg ggcccggact cgtgccataa
     4201 cgcaccagga agggcgaccc gcaccgctcg cacacgcccg gaaatccgtc ggccccgatg
     4261 acggtcttgc agcccgaaca ctcgagtgac cacggcagtc ggctcaccgt tgctccaggc
     4321 gatcgaggat gcggtcggcc agacgctggg aaaaccccgg taatgaggcg atctcttccc
     4381 gagtggccgt ccgaacgccg gccaccgagc cgaaccgctc cagcagtgcg cggcgccggc
     4441 ccggccccac cccgggaatg gcgagcaact cggaggtgat ggtgcgcttg gcacggcggg
     4501 tccggctata gctgccggca aagcgatgcg cctcgtccct ggcccgctgc agcagctgca
     4561 gcgcggggct acgccgcgag agccgcaacg gctccgcacg gccggggagg tacacctcct
     4621 cctcgcgctt ggcgagactg gcgagcggcg tcgccacccc cacgtcgttc gcggcggcga
     4681 ccgccgccga cagctgtccc cgcccgccgt ccaccacgac cagatccggc aacgccttct
     4741 gctcgtccaa ccgacggcgc aggaaacggg tcacgacctc gtggacggcc gcgaagtcgt
     4801 cct
//
LOCUS       DBNGZW010000666         7025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_540321, whole genome shotgun sequence.
ACCESSION   DBNGZW010000666 DBNGZW010000000
VERSION     DBNGZW010000666.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7025
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_540321"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..227)
                     /locus_tag="AC16JC_15685"
     CDS             complement(<1..227)
                     /locus_tag="AC16JC_15685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166190.1"
                     /translation="MMRHAARRIVWLAVATGLAACASPEAARTRGGGPGGDPGNRGAV
                     VRTHAGAKIYNRTPCRTPKVKCSGPMPAPGS"
     gene            complement(224..1135)
                     /gene="nrfD"
                     /locus_tag="AC16JC_15690"
     CDS             complement(224..1135)
                     /gene="nrfD"
                     /locus_tag="AC16JC_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR5166191.1"
                     /translation="MSDTFFTASPHWSWFIIPYFFIGGLAGGAYFLAALVEWFGRPED
                     RPVVRTGYAVAFWGAILSGLLLTLDLGRPLRFWHMLFQSERFPHLMFKSWSPISFGAW
                     AILLFGACAALSMLGARAEEGKIQGAAAKALSGGTVLGKLVAGVGGLFGFFVAGYTGV
                     LLAVTNRPIWADSPWLGALFLVSGASTGAAMLTMLGPGRGASERSLKWLTSFDSRALV
                     LELVILIAFMVSLGSVAKVWLSVWGVLLLGVVGVGILWPLRLHAKPAGAANAALLVLV
                     GGFLLRAVTILASSGIEFHRVAEGVVR"
     gene            complement(1135..1944)
                     /locus_tag="AC16JC_15695"
     CDS             complement(1135..1944)
                     /locus_tag="AC16JC_15695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5166192.1"
                     /translation="MSTEPMGFFTDTTVCIGCKACEVACKNWNELPSEDGGLRELSGD
                     SYDNTRRLDGIHWRHVRFIEQFHGAYEGRWLMMSDVCKHCVQAPCLEVCPTGAIIRTE
                     FDTVVIQSDVCNGCRDCIAACPFGVIDINHVSGTAQKCTLCYDRLQVGLEPACAKACP
                     TDSIQFGTIAELRARGQQRVGQLKSQGVDAYLYGADPGGPLGGLNSFYLLVDKPGVYG
                     LPENPRLPTRNLKPAGALSAASAAMLGLLALVGLRKQRMDDMAGTTSGGSA"
     gene            complement(1946..5197)
                     /gene="fdnG"
                     /locus_tag="AC16JC_15700"
     CDS             complement(1946..5197)
                     /gene="fdnG"
                     /locus_tag="AC16JC_15700"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA];
                     GO_function: GO:0047111 - formate dehydrogenase
                     (cytochrome-c-553) activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4622..4624),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HIR5166193.1"
                     /translation="MQVSRREFLKVTTGAGAGTAIGGLVGLGATLAPARAQAQELRIK
                     DAKSVPSICPFCSVGCATLVQVKDGRIVNIEGDPRSPHNEGTLCPKGAAIYQLHVNPN
                     RMTKVLHRKPGAGDWEEVELEWAMDRVSELIKQTRDETFIEQLPNGKRVNMTPAIFAL
                     GGATLDNEFNHVCQKFWRGIGVVAIENQARIUHSSSVPGLGARNGRGASTTYPRNLEH
                     TDCFIIMGSNMAECHPVAFRWPMKAKLNGAKLVHIDPRFTRTSAVADLHVPIRAGSDI
                     AFLGGIINHVVNSERWNSEPFFRDYTVNYTNLATIVNESFQDTEDLEGVFSGLLEYKE
                     PDFWPYNGNVGRYDNTTWQYDRGPKPPGPATEDVIKNRGSFDDLLRSLRKGTPRTDPT
                     LQHPRCVFQLLKKHFSRYTPEMVEQICGCPKEKFVRVAEMLLENSGPDRTSAFAYAVA
                     WTQHTYGVQIISCCALLSQLTGNMGRPGGGIMALRGHAAIQGSTDVPTLYHSIHGYLN
                     APSAVRPHDSLRAYLAAETPPTGYWANGPKWMVSYLKSVYGDAATPENDFGYDWHPKI
                     LGDHSHMATFAAMNEGKVRGTVVVGQNPATSLNGTATRRALRQLEWLVVKDNWLTETA
                     TYWYQAPEVKSGEVKVGDIKTEVFFFPSTQIAEYDGSYTNTQRMLQWHSFAAKAPGDC
                     RTDSWFYHNLAKRLKKAYADSTLPRDQGWKNVTWDFDPDPPEEPLYPGEISARKVLRE
                     INGYVTGNPAQHLPGFAALKDDGSTTCASWIYSGCFPAWDQNLTARREPDPPGVPGAH
                     LKWGWAWPANRRVIYNRAAADPRGNPWSERKRWVWWDPTVVNPPDPVTKKPVPPGKWV
                     GYDVPDFLLTVAPSHKGKPDGIIMEPLSGTEAFIMRPEGKGWIFVPSGLVDGPFPTYY
                     EPHESPVDNLLYPKQHASPVHKVWSPGKPWNQLASVGDAKFPYVITTYRLTEHYLSGS
                     MSRWLPWLAELQPELFIEIGTALAREKSIKNLDWVVVSSPRGQIRAKALVTDRIGVMN
                     VAGKRIHHVGMPWHWGWAGLSTGPVVNDLTAWVGDPNVSIHEGKAFVCNVERA"
     gene            complement(5445..6599)
                     /locus_tag="AC16JC_15705"
     CDS             complement(5445..6599)
                     /locus_tag="AC16JC_15705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166194.1"
                     /translation="MLRRPVGWSLPLLALVLQTSPALGQDVKAGQDISITIHGILSGT
                     LFAQDADFGLGNGQKAEFVTAERDKWVHGGDVRNMRLSLGLNGPAISGNWRANANFEM
                     DFFGPFASGGAFGDEQPLPRLRLAYVDATNGRTTVRFGQDWSLTLGNIPVSTSHIGFP
                     LGWGSGGFIGWRFTGVKFIQMLSAPGATTTTRLQLALFNGSWSDEPAGTDNQFTAGER
                     GSPQVEGRLDFGTAKWNFYAVGHVDSKDSINTAGDNLTSYDVEGGFSTTQGDLFLQGN
                     AHYGRGMGHHFAQIVQFGDIVGWGAWGQLGYTLNPKWTVWGYYGTENPDDADVIASGN
                     TRLRSWLTTAMLRYKVGPYAMGLEWLHNETALPADWLSGNQVLLSARFDF"
     gene            6741..>7025
                     /locus_tag="AC16JC_15710"
     CDS             6741..>7025
                     /locus_tag="AC16JC_15710"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF016362.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HIR5166195.1"
                     /translation="MAELPEVTPEYFRILVVRELRKAGFEVGDAGIRRRSELAEPERG
                     FVLELVVDLRIPGEGRRALVVCRRQDEPVRGEVIDALSSALVDARADSGMV"
BASE COUNT         1115 a         2363 c         2379 g         1168 t
ORIGIN      
        1 tacgacccgg gcgcgggcat ggggccagag catttcacct tgggagttcg gcagggcgtc
       61 cggttgtaga tcttggcccc cgcgtgagtt cgcaccaccg caccacggtt acccggatca
      121 ccgcccggac ccccaccccg cgtccgcgcc gcttccgggc tcgcacaggc cgcgaggccg
      181 gtcgcgacgg cgagccacac gatccggcgg gcagcatgcc tcatcatcgc accaccccct
      241 ccgccacgcg gtggaactcg atcccgctcg aggcgaggat cgtgactgcg cgcaacagga
      301 atcccccgac cagaacgagc agcgccgcgt tggcggcgcc cgccggcttt gcgtgcagcc
      361 gcagcggcca gagaatcccc acgccgacca caccgagcag cagcaccccc cagacgctca
      421 gccagacttt cgccaccgag cccaacgaca ccatgaaggc gatcaggatc acgagctcga
      481 ggaccagcgc ccggctgtcg aacgacgtga gccacttgag cgaccgctcg ctcgcgccac
      541 gcccggggcc gagcatcgtc agcatcgccg ccccggtgga cgcgccggac acgaggaaca
      601 gcgcccccag ccagggactg tcggcccaga tcggccggtt cgtgacggcc agcagcacgc
      661 ccgtgtaccc ggccacgaag aacccgaaca gccccccgac gccggccacc agcttgccca
      721 ggaccgtccc gccgctcagg gctttcgccg ccgctccctg gatcttgccc tcctcggcgc
      781 gggcgccgag catcgacaac gccgcgcacg ccccgaagag gagaatcgcc cacgcgccga
      841 aggagatcgg cgaccacgac ttgaacatga ggtgcgggaa gcgctccgac tggaacaaca
      901 tgtgccagaa ccgcagcggc cgtccgagat cgagtgtgag caggagcccg ctcaggatgg
      961 cgccccagaa ggccacggca tagcccgtcc ggaccacggg ccggtcttcc ggacggccga
     1021 accactcgac cagcgcagcg aggaagtacg cgccgccggc cagtcccccg atgaagaagt
     1081 agggaatgat gaaccagctc caatgggggg aggccgtgaa gaaggtgtcg gacattaggc
     1141 gctccctccc gatgtcgtgc cggccatgtc atccatccgc tgcttgcgca ggccgaccag
     1201 cgccagcagg ccgagcatcg cggcgctggc ggcggagaga gcgccggccg gcttcagatt
     1261 ccgggtcggg agccgcgggt tctccggcag gccgtagacc cccggcttgt ccaccaggag
     1321 atagaaggag ttgagtccgc caaggggccc gccgggatcg gcgccgtaca ggtaggcgtc
     1381 gacaccctgg gacttgagct gcccgacgcg ctgctggccg cgcgcgcgca actccgcgat
     1441 ggtgccgaac tggatcgagt cggtcgggca cgccttggcg caggccggct ccagcccgac
     1501 ctgcagccgg tcgtagcaca gcgtgcactt ctgcgccgtg cccgacacgt gattgatgtc
     1561 gatcacgccg aagggacagg cggcgatgca gtcgcggcag ccattgcaca cgtccgactg
     1621 gatcacgacc gtgtcgaact cggtgcggat gatggcgccg gtgggacaga cctcgaggca
     1681 cggcgcctgc acgcagtgct tgcacacgtc gctcatcatc agccagcggc cctcgtaggc
     1741 gccgtggaac tgctcgatga accggacgtg tcgccagtga atgccgtcga gccgccgcgt
     1801 attgtcgtag ctgtcgccgc tcagctcccg caacccgccg tcctccgagg ggagctcgtt
     1861 ccagttcttg cacgcgactt cacacgcctt gcacccgatg cacaccgtgg tgtcggtgaa
     1921 gaatcccatg ggctctgtgc tcatgtcagg ccctttccac gttgcacacg aaggccttgc
     1981 cttcgtggat cgacacgttg ggatcaccga cccaggcggt gagatcgttg accaccgggc
     2041 cggtcgaaag ccccgcccag ccccagtgcc acggcatccc gacgtggtgg atgcgtttcc
     2101 cggcgacgtt catcacgccg atccggtcgg tcacgagcgc tttggcgcgg atctgtccgc
     2161 gcggactcga gaccaccacc cagtcgagat tcttgatgct cttctcccgg gccagcgcgg
     2221 taccgatctc gatgaacagc tccggttgca gctcggcgag ccacggcagc cagcggctca
     2281 tcgagcccga gaggtagtgc tcggtcagcc ggtaggtcgt gatgacgtag gggaacttcg
     2341 cgtcgccgac ggaggcgagc tggttccagg gtttgccggg cgaccagacc ttgtgcacgg
     2401 ggctcgcgtg ctgcttcggg tacaggagat tgtcgaccgg cgactcgtgc ggctcgtagt
     2461 acgtggggaa gggtccgtcc accagcccgc tcgggacgaa gatccacccc ttcccttccg
     2521 gccgcatgat gaacgcttcc gttcccgaga gcggctccat gatgatgccg tcgggcttgc
     2581 ccttgtggct cggggccacg gtgaggagga agtccggcac gtcgtagccg acccacttgc
     2641 cgggcggaac cggcttcttg gtgaccggat cgggcgggtt gacgaccgtg ggatcccacc
     2701 acacccagcg cttacgctcg ctccacgggt tgccccgcgg atccgcggcg gcgcggttgt
     2761 agatgacgcg tcggttggcg ggccatgccc agccccactt gagatgcgcg cccggcacgc
     2821 cgggaggatc gggctcgcgc cgcgcggtga ggttctgatc ccaggccggg aagcacccgg
     2881 agtagatcca cgacgcgcaa gtcgtcgagc cgtcgtcctt gagcgccgcg aagccgggca
     2941 ggtgctgcgc cggattgccc gtcacgtagc cgttgatctc ccgcagcact ttgcgcgcgc
     3001 tgatctcgcc cggatagagc ggctcctcgg gcgggtccgg atcgaagtcc caggtgacgt
     3061 tcttccagcc ctggtcccgg ggaagcgtgc tgtccgcgta cgccttcttg agacgcttcg
     3121 ccaggttgtg gtagaaccag gaatcggtgc gacaatcgcc cggcgccttg gcggcaaagg
     3181 aatgccactg cagcatgcgc tgggtgttgg tgtagctgcc gtcgtattcg gcgatctgcg
     3241 tggaggggaa gaaaaacacc tcggtcttga tgtctccgac cttgacctca ccgctcttca
     3301 cttccggcgc ctgataccag tacgtggccg tttcggtgag ccagttgtcc ttgacgacca
     3361 gccactcgag ctggcgcagc gcgcgccgcg tcgccgtgcc gttgagcgac gtggcgggat
     3421 tctgcccgac gactaccgtc cccctgacct tcccctcgtt catggcggcg aacgtcgcca
     3481 tgtgcgagtg atcgcccagg atcttggggt gccagtcgta gccgaagtcg ttctcgggcg
     3541 tcgcggcgtc gccgtacacc gacttgaggt acgacaccat ccacttcggc ccgttggccc
     3601 agtagccggt gggcggcgtc tcggcggcga ggtaggcgcg cagcgagtcg tgcgggcgga
     3661 cggccgaggg cgcgttgagg tagccgtgga tgctgtggta cagcgtgggg acgtcggtcg
     3721 agccctggat cgcggcgtgg ccgcgcagcg ccatgatccc gccgcccggc cgccccatgt
     3781 tcccggtcag ctgcgacagc agcgcgcagc agctgatgat ctgcacgccg taggtgtgct
     3841 gcgtccaggc gaccgcgtag gcgaaggcgc tggtgcggtc cgggcccgaa ttctcgagca
     3901 gcatctcggc gacccggacg aatttctctt tggggcaacc gcagatctgc tccaccatct
     3961 caggggtgta gcggctgaag tgcttcttca ggagctggaa gacgcagcgc gggtgctgca
     4021 gcgtgggatc ggtccgcggc gtccccttgc gcagcgagcg cagcaggtcg tcgaaggagc
     4081 cgcgattctt gatcacgtct tccgtcgccg gaccgggcgg cttgggcccc cggtcgtact
     4141 gccaggtcgt gttgtcgtag cggccgacgt tcccgttgta gggccagaag tcgggctcct
     4201 tgtactccag taggcccgag aacacgccct cgaggtcttc ggtgtcctgg aaggactcgt
     4261 tgacgatcgt ggcgagattg gtgtagttga ccgtgtagtc gcggaagaac ggctccgagt
     4321 tccaccgctc gctgttcacg acgtggttga tgatcccgcc gaggaacgcg atgtccgatc
     4381 cggcacggat cgggacgtgc aggtcggcca ccgcgctggt gcgcgtgaag cgcggatcga
     4441 tgtgcaccag cttcgcgccg ttcagcttcg ctttcatggg ccagcggaag gccaccggat
     4501 ggcactccgc catgttcgat cccatgatga tgaagcaatc ggtatgttcg aggttgcgcg
     4561 ggtacgtcgt cgaagcgccg cgaccgtttc gtgcccccag accggggacg ctagagctgt
     4621 gtcatatcct ggcctggttt tcgatggcga cgaccccgat gccgcgccag aacttctggc
     4681 acacgtgatt gaactcgttg tccagggtcg ccccgcccag cgcgaagatc gcgggcgtca
     4741 tgttcacgcg cttgccgttg ggcagctgtt cgatgaacgt ctcgtcgcgc gtctgcttga
     4801 tcaactccga gacgcgatcc atcgcccact cgagctccac ctcttcccag tccccggcgc
     4861 ccggcttgcg atgcaacacc ttggtcatcc ggttcggatt cacgtgcagc tggtagatcg
     4921 ccgcgccctt ggggcacagt gtcccctcgt tgtgcgggct gcgcggatcg ccttcgatgt
     4981 tgacgatccg gccgtccttc acctgcacca gggtcgcgca ccccaccgag cagaacgggc
     5041 agatgctggg caccgatttg gcgtccttga tgcgcagctc ctgcgcctgc gcgcgggccg
     5101 gcgcgagcgt cgcgccgagg cccaccagtc cgccgatcgc cgtgccggcg cctgcgccgg
     5161 tcgtcacctt gaggaattcg cgtcgactga cctgcatagg acgatcctcc ccgttctagg
     5221 acaggggttc ggacagcgcg gctgcgtggg gcgtggacag gcggagcgcc ggcgggtaaa
     5281 cggcaggttc ggaatggatc gcgacgaggt atgtcatcgg cgcctccgaa gagcgcgggg
     5341 ccgctctgac cggcggccgc gggctgcagc cgtgatatag ggttgcttca tgttcaccgc
     5401 aaggctggtt gggaggcccg cggcccccaa ccagccgcgc gtccctagaa atcgaagcgg
     5461 gcgctgagca gcacctggtt gcccgacagc caatcggccg gcagcgccgt ctcgttgtgc
     5521 agccattcga ggcccatcgc gtacggcccg accttgtacc gcagcatcgc cgtcgtcagc
     5581 cagctccgca gccgcgtgtt cccggaggca atcacatcgg cgtcgtcggg gttttcggtc
     5641 ccgtagtagc cccacacggt ccacttcggg ttcagcgtgt aacccaactg cccccaggcc
     5701 ccccacccca cgatgtctcc gaactggacg atctgagcga agtggtggcc catcccccgc
     5761 ccatagtgcg cgttcccctg gagaaagagg tcgccctggg tggtgctgaa gccgccttcg
     5821 acgtcgtagc tcgtgaggtt gtcgccggcc gtgttgatgg agtccttcga gtccacgtgg
     5881 ccgacggcgt agaagttcca cttggcggtt ccgaagtcga gacgcccctc gacctgcggc
     5941 gagccccgct cgcccgcagt gaactggttg tccgtgccgg ccggctcgtc gctccacgag
     6001 ccgttgaaga gggccagctg cagccgcgtg gtcgtcgtgg cgccgggggc gctcagcatc
     6061 tgaatgaact tcaccccggt gaagcgccag ccgatgaagc cgcccgatcc ccatcccagc
     6121 gggaagccga tgtgcgaggt cgacaccggg atgttgccga gcgtgagcga ccaatcctga
     6181 ccgaagcgca ccgtggtccg gccgttcgtg gcgtccacgt aggcaagccg caggcgcggc
     6241 agcggctgtt cgtccccgaa cgcgcccccg ctcgcgaacg ggccgaagaa atccatctcg
     6301 aagttggcgt tggcccgcca gttccccgag atagccggtc cgttgagacc gagcgacaga
     6361 cgcatgttgc gcacgtcgcc gccgtgcacc cacttgtcgc gctccgcggt gacgaactcc
     6421 gccttctggc cgttgccgag tccgaagtcc gcgtcctgcg cgaagagcgt gccgctgagg
     6481 atgccgtgga tcgtaatcga gatgtcctgg ccggctttga cgtcctgccc gagtgcgggt
     6541 gatgtttgca ggacgagcgc cagcaggggg agggaccacc caacaggtct gcggagcatg
     6601 ggaacctcct gagcagcgtg tcggtggatc gaaccggtcg tgtgaccgac cggtaggcga
     6661 cgcggcccaa ggggccgcgt ggcggccagc attatgtctc acccgaaagc cgccgatggt
     6721 caagggggcg gaggggcgcc atggccgagc tgcccgaggt gacgccggag tacttccgga
     6781 ttctggtcgt gcgcgagctg cgcaaggccg gattcgaagt gggggatgcc ggcatccgcc
     6841 gacgttccga gctggccgag ccggaacggg gcttcgtgct ggagctggtg gtagacctca
     6901 gaataccggg cgagggccgg cgcgcactcg tggtctgccg gcggcaggac gagccggtgc
     6961 gcggcgaggt catcgatgcg ctgagttcgg cgctggtaga tgcccgggcc gacagcggga
     7021 tggtc
//
LOCUS       DBNGZW010000667         4088 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_729319, whole genome shotgun sequence.
ACCESSION   DBNGZW010000667 DBNGZW010000000
VERSION     DBNGZW010000667.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4088)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4088)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4088
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_729319"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3465
                     /locus_tag="AC16JC_15715"
     CDS             <1..3465
                     /locus_tag="AC16JC_15715"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5166196.1"
                     /translation="GVASFSGLSIDKAGTSYTLLATSFPALTSATSDEFNIAPAAPSR
                     AHFFVEPSSATAGVAITPDIQVEILDAFGNRVGTAINSVIVGLLANSGGGGTTLAGTK
                     THNASAGVATFPGLSINKAANGYTIRATSSGLASDTSVAFDITPGPATQLAFTTQPTS
                     TAAGATISAVVVTARDALGNTATGFAGDVAMDIGTNPGSGILTGTLSVPAVSGVATFS
                     NLSIDKVGTGYTLSASSPGLTSATSATFNITVGAAAKLAFGQQPTSTTGGTAIAPAMT
                     VRILDANGNLTGSSASVTLAITSGTGTAGAHLAGTVTRAALAGVATFNDVNIDSAGTA
                     YTLNATSSGLTADTSTAFNVAVGSSAKLDFLVQPSTTPPGGTITPAVQVEIRDAGGNL
                     VTTDNTTSVTVAIGTNPSGGTLGGTKTRTAVAGVATFSNLSINNAGTGYTLTAAATSL
                     VGATSNSFDIVVGTATKLAFFVQPSGTTGGATISPAVQVEIQDAGSNRVTTATNTVTL
                     AFGTNPKSGHLSGTKTVAAVSGVATFADLSIDSAATGYTLSAGSSGLTGATSNPFDIT
                     VGDANKLGFRVQPSNAPGGANITPAVQVEIRDAGGNRVTTAGDSITMAIGTNPSNGTL
                     SGTTTLAASNGLATFSTLSIDSAGTGYRLSATASGLSSATSNTFNITVGAAAKLGFLV
                     QPSDAAAGSAITPAVKVEILDAGGNRVATATNNVTVAIGTNPGGGALGGTKTVAAVSG
                     VATFSNLSINKTGVGYTLQTTSGTLTPATSTSFDIGADGVDGSLSTLVSATDTIGQCL
                     FSCAPGIRSSTVTVTVRDQLGNLVSGTPVVLSASGTGNAFAPSATGNTDANGVFTAAF
                     NASDFGTRTVSATAGGVGISQTAAVAVMPVLVGAGDIADCNSVRDDATASQLDSIPGT
                     VFAAGDNAYPNGRAADFTNCYDPTWGRQKARTKPVVGNHEYDSSGTAAAYFSYFTPAV
                     ADPLGNGFGYYSFELGTWHVVVLNSDSGVTSPVAGPAQLSWLQSDLAGRSNQCVLAIW
                     HRPLFTSGSSGGAGIRVRKLWQALEDAGAEVVINGHDHLYERFAPQDSLGNATSAGIR
                     EFIAGTGGGETHSNYVNSPANVEASDAGNFSRGVLRLTLYAQSYRWEFLPAQGQGTYT
                     DSGTQTCH"
     gene            complement(3479..>4088)
                     /locus_tag="AC16JC_15720"
     CDS             complement(3479..>4088)
                     /locus_tag="AC16JC_15720"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR5166197.1"
                     /translation="RAGRYVLLTFDDGGRSALTAAELLARYGWRGHFFVVTSLTGSRG
                     FLDAAGIRELRRAGHLIGSHSHTHPGIFREQSPQRMVEEWRTSCDMIAQILGEPCVAA
                     AVPGGDISRRVLESASTAGLRYLFTSEPWLAPRRMGDCWILGRHSVKVATSAARVSEL
                     VRFRGWSRALLRRRLKVFVTSAVPPLYRLYVRARTRQGPQPA"
BASE COUNT          706 a         1434 c         1384 g          564 t
ORIGIN      
        1 ggcgtggcct cgttctctgg attgagcatc gacaaggccg gcacgagcta caccctgctg
       61 gccacgtcct tcccggcgct gacgtcggcc acctccgacg aattcaacat cgcgccggcg
      121 gcgcccagcc gggcgcactt cttcgtggag ccgtccagtg ctacggccgg cgtcgcgatc
      181 acgcccgaca tccaggtcga gatcctggac gccttcggca accgcgtcgg gacggccatc
      241 aacagcgtga tcgtcgggct gctcgccaac tccggcggcg gcgggacgac gctcgccggc
      301 accaagaccc acaatgcctc ggcgggcgtg gcgaccttcc ccggcctcag catcaacaag
      361 gcggcgaacg ggtacaccat ccgggcgacg tccagcggcc tggcctccga tacgagcgtg
      421 gcgttcgaca tcacgcccgg cccggcgaca cagctcgcct tcaccacgca gcccaccagc
      481 accgctgcgg gggcgaccat ttcagccgtg gtggtgacgg cccgggatgc tctggggaat
      541 accgcaaccg ggttcgccgg cgacgtcgcc atggacatcg ggacgaaccc ggggagcggg
      601 atcctcacgg gcacgctcag cgtaccggcc gtgagtgggg tggcgacgtt ctcgaacctc
      661 agcatcgaca aggtcgggac cggctacacc ctgagcgcct cgtcgcccgg gctgaccagc
      721 gccacgtcgg cgacgttcaa tatcaccgtc ggagccgcgg cgaagctggc tttcgggcag
      781 cagccgacct cgacgaccgg cggcacggcc atcgcgcccg ccatgaccgt gcgcatcctc
      841 gatgccaatg gcaacctgac gggatcgagc gccagcgtca cactggccat cacctcggga
      901 acggggacgg ccggggcaca cctcgccggg accgtcacca gggctgcgct tgccggcgtc
      961 gcgacgttca acgacgtcaa catcgacagc gccggcaccg cctacacgct gaatgcgacg
     1021 tccagcggtc tgaccgccga cacctcgacg gccttcaacg tcgccgtcgg cagcagcgcg
     1081 aaactcgact tcctggtgca gccttccacg acgccccccg gaggaacgat caccccggcg
     1141 gtccaggtcg aaatccggga cgcggggggg aacctggtga cgacggacaa cacgacgagc
     1201 gttaccgtcg ccatcggtac caacccgtcg ggtggcacgc tgggcgggac gaagacgcgg
     1261 accgccgtgg ccggtgtggc gacgttctcg aacctgagca tcaacaacgc cggaaccggc
     1321 tacaccctga ccgcggcggc tacatccctc gtcggggcga catcgaacag cttcgacatc
     1381 gtcgtcggca cggcaaccaa gctcgcgttc tttgtccagc cgtccggcac gaccggcggg
     1441 gcgacgatca gcccggcggt gcaggtcgag attcaggacg cgggcagcaa ccgcgtcacc
     1501 acggcgacga acaccgtcac cctcgcgttc ggcaccaacc cgaaaagcgg tcacctctcg
     1561 gggacgaaga cggtggcggc ggtgagcggt gtggcgacgt tcgccgattt gagcatcgac
     1621 agcgccgcga ccgggtacac cctgagtgcg ggctcctccg gtctcaccgg ggcgacatcc
     1681 aatccattcg acattacggt cggtgacgcc aacaagctcg gcttccgggt gcagccgtcg
     1741 aatgccccag gcggcgccaa catcaccccg gccgtgcaag tggagattcg cgatgccggc
     1801 ggcaatcggg tgacgaccgc cggcgacagc atcaccatgg cgatcggcac caacccgagc
     1861 aacggcacgc tgtcggggac gacgacgctc gccgcgtcga acggcctggc gacgttcagc
     1921 accttgagca tcgacagcgc ggggaccggc taccggctct ccgcgacggc atccgggctg
     1981 tcgagcgcga cctcgaacac cttcaacatc acggtcggcg ccgcggcgaa actcggattc
     2041 ctggtccagc cgtcggacgc cgcggcgggg tcggccatca cccccgcggt caaagtggaa
     2101 atcctcgatg ccggcggcaa ccgggtggcg accgctacca acaacgtcac ggtcgccatc
     2161 ggcaccaacc ccggcggggg tgcgctgggc ggcaccaaga cggtcgcggc cgtgagcggc
     2221 gtggccacct tctcgaacct cagcatcaac aagaccggtg tgggctacac gctccagacg
     2281 acctccggga cgctcacccc ggcgacgagc acgtcgttcg acatcggcgc ggacggcgtc
     2341 gacggctcgc tttccactct ggtctccgcc acggacacga tcgggcagtg cctgttctcg
     2401 tgcgcgccgg gcatccggtc atcgacggtg acggtgacgg tccgggacca gctcggaaac
     2461 ctggtcagcg ggacaccggt ggtcctgtct gccagcggca ccggaaacgc ctttgcccct
     2521 tcggccacgg gcaataccga cgcgaacggc gtgtttacgg cggctttcaa tgcgtcggac
     2581 ttcgggacga gaacggtgtc ggcgacggcc ggcggggtgg ggatcagcca gacggctgct
     2641 gtggcggtca tgccggtgtt ggtcggggcg ggggacatcg ccgactgcaa ttcggttcgg
     2701 gacgacgcga cggccagtca gctggacagc attccgggca cggtgttcgc cgccggcgac
     2761 aacgcgtatc ccaacggcag ggcggcggac ttcacgaact gctacgaccc gacctgggga
     2821 cggcagaagg cacggaccaa gccagtcgtg gggaaccacg agtacgacag cagtggcacc
     2881 gcggcggcat acttcagcta cttcacgccc gcggtggcgg atcccctggg caacggattc
     2941 ggctattaca gcttcgagct tggcacctgg catgtggtgg tgctcaacag tgattcgggc
     3001 gtcacgagcc ctgttgcagg tccggcgcag ctcagctggc tgcagagcga tctggcgggc
     3061 cgctcgaacc agtgcgtgtt ggcgatctgg caccggccgt tgttcacctc cggctcatcg
     3121 ggtggggcgg ggatcagggt gcgcaagctg tggcaggcgc tggaggacgc cggcgcggag
     3181 gtcgtcatca acggacacga ccacctgtac gagcggttcg cgccgcagga ctctctcggc
     3241 aacgcgacgt ccgccggcat ccgggaattc atcgcgggga ccggcggggg tgaaacacac
     3301 tcgaactacg tcaatagccc ggccaacgtc gaggccagcg atgccggcaa cttctcgcgg
     3361 ggggtgctgc gcctcaccct ctacgcccag tcgtatcgtt gggaattcct gcccgcccag
     3421 ggacagggaa cgtacacgga ctcaggcacg caaacctgtc actgaggacg gggctgcgtc
     3481 aggcgggctg ggggccctga cgcgtgcggg cgcggacgta gagccggtac agcggcggca
     3541 cggcgctggt gacgaacacc ttgaggcgcc ggcgtagcag ggcccgggac cagccccgga
     3601 atcggacgag ctccgacacc cgtgccgcgc tggtggcgac cttcacgctg tggcggccca
     3661 ggatccagca gtcgcccatg cgccgcggcg cgagccacgg ctccgacgtg aacaggtagc
     3721 gcaggccggc cgtactcgcc gactcgagga cgcggcgcga gatatcgccg cccggaaccg
     3781 cggccgcgac gcacggctcg cccaggatct gcgcgatcat gtcgcagctc gtgcgccact
     3841 cctccaccat ccgttgcggg gactgctcgc ggaagatgcc gggatgggta tgcgagtggc
     3901 tgccgatcag atggccggcg cggcgcagct cccggatccc cgccgcgtcc aggaacccac
     3961 ggctcccggt gaggctcgtc acgacgaaga agtgcccccg ccagccgtat cgggccagca
     4021 gctcggccgc cgtgagcgcg ctgcgccccc catcatcgaa cgtcaacagc acatagcgac
     4081 ccgcccgg
//
LOCUS       DBNGZW010000668         9884 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1751353, whole genome shotgun sequence.
ACCESSION   DBNGZW010000668 DBNGZW010000000
VERSION     DBNGZW010000668.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 9884)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9884)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9884
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1751353"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            37..438
                     /locus_tag="AC16JC_15725"
     CDS             37..438
                     /locus_tag="AC16JC_15725"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR5166198.1"
                     /translation="MRVADIMRTNLKTIDAEASVADAVAALTEYSVSALPVLDRHGRA
                     VGVCSTKDIIAAEARCHDPAAREQLFEKTLVLEVMTPWPATAHPDEDVRKVAKRLLEE
                     KVQRLFVEEKGALVGVVSQTDIVAAVAATRV"
     gene            complement(455..1207)
                     /locus_tag="AC16JC_15730"
     CDS             complement(455..1207)
                     /locus_tag="AC16JC_15730"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5166199.1"
                     /translation="MSGPYDPHLTIRGEGAPLVLVPGMDGTGRLFYRQVPLLAPRYRV
                     ATYALRDDAADMDVLVEDLARVIDAAANGPGSAIVIGESFGGTLALSFALAHPERVRA
                     LVVLNSFSRFLPQVNLRLAITALRLLPWETMPLVRRLTAFRLHSRYTHRAEVRRFLEV
                     TRETRRDGYVARLRILQQYDARERLGTLRMPTLFLASELDHLIPSVREGRYMAERVPG
                     ATLRVLRGHGHICLLAPNLDLEQELRDWRPDL"
     gene            1206..1940
                     /locus_tag="AC16JC_15735"
     CDS             1206..1940
                     /locus_tag="AC16JC_15735"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HIR5166200.1"
                     /translation="MYTLNKLGRLAWAVGTAFAVECVVFGLSVLPATLFWEWTFRWSL
                     PANWIRIVLLSMSLVPAYLLFAVALMTLTAAALRLVGWRTAPHAELRIVDLEWPLLDW
                     ARYAMATHVVRVFAGTLLRATPLWTLCLRLNGARIGRRAFVNSVTLSDHNLLECGDDV
                     VIGAGVYLSGHTVERGVVRTAPVRLGPGVTIGVGSVIGIGVSIGAHTQVGALSVVPKH
                     RTLEPDAVYGGVPVRRLDVPEVIETA"
     gene            complement(1937..2791)
                     /locus_tag="AC16JC_15740"
     CDS             complement(1937..2791)
                     /locus_tag="AC16JC_15740"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5166201.1"
                     /translation="MKVRRVLGAVSLYTPSPKAVLTAAEVAAAFHAELTVLTVLRDPW
                     ELVRADEVEGLRRAKSGSPAELAAARAIERLQALCGPAVLPARSASYRAAFGWPSIEV
                     ARCADEIGADLIVLGKGEEVARGAAENVTTATMRRCRVPVLVAPAAHRPYRRVLACVD
                     DSPHARDVLEAAQTAADGFGAHLVALHVEPAEAGVLAHGERRSWLRHVEQEGSGQSGG
                     VVAACETIVRQGDVTSEILAECRAEDAELLVFGYRRGMNYGDPGAVTTVAARLLRRAC
                     CALLAVPV"
     gene            2939..3895
                     /locus_tag="AC16JC_15745"
     CDS             2939..3895
                     /locus_tag="AC16JC_15745"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR5166202.1"
                     /translation="MSWKRILVGVDESPQAAAAAALAVRLAKALRGECIPVHAVRQMW
                     LAFAEEEIVDRSAELQAALIDGSRRKVAAALQGHIPEETLRRLIVRPGSPAAVLRDVA
                     RDVEADAVVLGGKHHLALRRWAGGSTAHNCVRTIGLPVLVAGPRAETGGAPFGRILGA
                     VDLSAAAAPVAAAARELAEGLRAELRALCVIEPPVPLPDVTSVLTPSQYSRLAEHALT
                     RRVWPLFRGSEAKTVARQGPVMETIREESAAWNADLLVVGSHGKGWGERLILGSATER
                     LLEDLPTSLLVVPVHAPEATRKPTRPPARRAVKKPVRAGSHA"
     gene            3895..4776
                     /locus_tag="AC16JC_15750"
     CDS             3895..4776
                     /locus_tag="AC16JC_15750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015352148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR5166203.1"
                     /translation="MPDAQNVAVAARLEELAALLERQGANPFRVEAYRNAADQVRRLE
                     RPAGDILRSEGSGGLERLPAIGTTIARAIRDLVETGRLPMLDRLRGESDAVTLLSSVP
                     GIGRTLAERLHHDHGIDTLEQLEAAAHDGRLAAIRSFGEKRLLGVREALGARLGRVPR
                     SAVVSAEPAVDELLRVDREYRTRAAAGTLRTIAPRRFNPRREAWLPILHTMQGERHYT
                     ALFSNTARAHDLGKTREWVVIYVDGGRGERQYTVVTASRGPMKGKRVVRGREVECQAV
                     YGERAATRTPSESLSVG"
     gene            complement(4722..5882)
                     /locus_tag="AC16JC_15755"
     CDS             complement(4722..5882)
                     /locus_tag="AC16JC_15755"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR5166204.1"
                     /translation="MNLNRRAMLWGGAGVAAAAFLGYTFRPTAIPVEVAAVERGALRV
                     TIDEEGRTRVRDRYVVVAPAAGRVARIALEEGAPVTRGMVVARLSAAPLDPRSREQAV
                     ARLDGAEDAQRAAAAAVSEARAALDQATRNRARAESLFAKNLLAPAQREEAALAETTA
                     VRRFQAADFTAQAAAHDVEQARAVIAGGGGSALALVSPVRGRVLRIPERSERVVAAGT
                     PLLELGDPTRLEIVVDLLSEDAVKVRPGDRMLVADWGGERPLEAHVRRVEPSGFTKVS
                     ALGVEEQRVNVIANLDETPPQLGDGYRVETQVVLWEGTALKVPSSALFQDRDQWRVFL
                     VRDGRARSRAVQVGHRNPFDVEITGGLTAGDVVIRHPTDKLSDGVRVAARSP"
     gene            complement(5887..8253)
                     /locus_tag="AC16JC_15760"
     CDS             complement(5887..8253)
                     /locus_tag="AC16JC_15760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015144091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5166205.1"
                     /translation="MVTALDRKALRDLWHLRGQMAAVALIVACGVASIVTIRTAYDSL
                     KGSLDAYYRDYRFADVFASLRRAPDPLAAKIAALPGVAAVQSRVVVHVTLAVPGLAEP
                     ATGRLVSVPERRVPMLNDLHLRQGRYIAPGRSDEVVASEQFAQANRLQVGDTLGAIIN
                     GRWQRLRIVGIALSPEFVYQISPGWFFPDDKRYGVFWMGHEALTTAFDMQGAFNDVSL
                     RLTAGADEREVRVSLDRLLAPYGGLGAIGRADHQSNRFITDEIAQLRVSGAIVPVIFL
                     GVAAFLVHIVLARLVATQRPQIGVLKAFGRSDVDVGLHYAKLVVGVELLGAAVGAAFG
                     MWLGGRLTVLYAAYYRFPVLHFTAGWGVLALATLVSAGSGLLGALSAVGRAVRLPPAE
                     AMRPEPPPRYRRGAFEALGLHRLFGPVGRMVLRTLERRPGRAIVTAAGIGLACGIIVL
                     GRFTIDAVEALADFEFDVRERQAVSISFTEPRPARVRYEVRHLPGVLRAEPFRDVGVR
                     LIAGHRSRRTVILGLEPDAELRQVADLDGRRRSLPAEGVLLTDKLAQVLGVAPGDPVT
                     VEVLEGARPTLVVPVVGLVHEPMGTLAYMDAGGLHRLLREGGTVSGAALAVDARAAPA
                     LYDALKHLPAVAAVSIREPTVQSFDETLARSHGIVTTYIVAFAVLIAFGVVYNTARIA
                     LSERARELASLRVLGFTRAETSRILLDEQAVLVLAALPLGFVFGYGGAALIVMAFDTE
                     LYRLPFVLHPATYGYAVIVILLAALASALIVRRRIDRLDLVGVLKTGD"
     gene            complement(8247..9011)
                     /locus_tag="AC16JC_15765"
     CDS             complement(8247..9011)
                     /locus_tag="AC16JC_15765"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018864806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5166206.1"
                     /translation="MMAAPAPFVSSVGATGGPSPAEPVFRAHDLSKVYRLGEVEVHAL
                     RSLSLELYRGEFVVILGPSGSGKSTLLNILGGLDVPTSGTVRFFDHDLTAAGERERTR
                     FRREHVGFVFQFYNLIPSLTARENVQLVCETAPRPLNPVRALELVGLGERLDHFPAQL
                     SGGEQQRVAIARAIAKQPDVLLCDEPTGALDFQTGKLVLEVLERVNRDLRTTVAIITH
                     NVAIGRMGDRVVHMHSGQIASIDRNPVRASVDTLQW"
     gene            complement(9078..9602)
                     /locus_tag="AC16JC_15770"
     CDS             complement(9078..9602)
                     /locus_tag="AC16JC_15770"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5166207.1"
                     /translation="MEARHYAAADLLRDGAPVWIRAIQPDDRERLARFHGRLSPESVY
                     LRYFEYKPVLSARDLTYLTELDYDRRVALVAALSPDRRAPIVGVARYDVLPAQPDQPV
                     RAELGIIVEDTFQNRGIGTVLLKHLLGVAHDHGIAEITAEVLPQNTRMLELVAGAGVP
                     VRRELDEGVIRLTV"
     gene            9683..>9884
                     /locus_tag="AC16JC_15775"
     CDS             9683..>9884
                     /locus_tag="AC16JC_15775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166208.1"
                     /translation="MPAKRPSRSETPPRPARPSGDSRRLEDRFRHLVAASARIFSQHT
                     LPAVLQEVVDAARGIVGARYAAL"
BASE COUNT         1498 a         3503 c         3551 g         1332 t
ORIGIN      
        1 cggcgtggtc gcgggcgcca agctctagga ggacgtatgc gagtcgctga catcatgcgc
       61 accaacctga agaccatcga cgccgaggcc tcggtggcgg acgcggttgc ggcgctgacc
      121 gaatacagcg tgtccgccct gccggtcctc gaccgccatg ggcgcgccgt gggcgtctgt
      181 tccaccaagg acatcatcgc ggcggaggca cgctgtcacg acccggctgc gcgggagcag
      241 ctgttcgaga agacgctggt gctggaggtc atgacgccgt ggccggccac cgcgcacccg
      301 gacgaggacg tgcgcaaggt ggcgaagcgc ctgctggagg agaaggtcca gcgcctgttc
      361 gtggaggaga agggcgccct ggtcggcgtc gtctcccaga cggacatcgt cgcggcggtg
      421 gcggccaccc gggtgtagcg gcccggatca cctttcagag atcggggcgc cagtcgcgca
      481 gctcctgctc cagatccagg ttcggggcga ggagacagat gtggccgtgc ccgcgcagca
      541 cccgcagcgt cgccccgggc acgcgctcgg ccatgtagcg cccctcccgc accgagggaa
      601 tcaaatggtc gagctccgac gccaggaaca gggtcggcat ccgcagcgtg ccgaggcgct
      661 cgcgggcatc gtactgctgc aggatgcgca ggcgcgcgac atagccgtcg cgccgggtct
      721 cccgggtcac ctcgagaaac cgccgcacct ccgcgcgatg cgtgtagcgc gagtgcagcc
      781 ggaacgcggt cagccggcgc accagcggca tcgtctccca gggcaggagc cgcagcgcgg
      841 tgatggcgag ccgcaggttg acctgcggca gaaaccggga gaaggaattg agcaccacca
      901 gcgcccgcac gcgctcgggg tgcgccagcg cgaagctcag cgcgagggtt ccgccgaacg
      961 attctccgat cacgatcgcg gaaccgggac cattcgccgc cgcatcgatg acgcgcgcca
     1021 gatcctcgac cagcacgtcc atgtcggcgg cgtcatcgcg cagcgcgtag gtcgccacgc
     1081 ggtagcgcgg ggcgagcagc gggacctggc ggtagaacag acgccccgtg ccgtccatcc
     1141 ccggcacgag cacgagcggc gctccctcgc cccggatggt caggtgcgga tcgtatggcc
     1201 cggacatgta cacgctaaat aagctcggca ggctcgcgtg ggcagtcggc accgcgttcg
     1261 cggtggagtg cgtcgtgttc gggttgtcgg tgctcccggc caccctgttc tgggaatgga
     1321 cgtttcgctg gtcgctgccc gccaactgga tccggatcgt gctgctgtcg atgtcgctcg
     1381 ttccggccta tctcctgttc gccgtcgcgc tgatgacgct cacggccgcc gcgctgcgac
     1441 tcgtcggctg gcgcaccgcg ccccacgccg agctgcgcat cgtggacctc gagtggccgc
     1501 tactcgactg ggcacgctac gccatggcga cccacgtcgt acgggtgttc gcgggcacac
     1561 tgctccgcgc cacgccgctg tggaccctct gcctgcgcct gaacggcgcc cggatcggcc
     1621 ggcgcgcctt cgtcaacagc gtcacgctgt cggaccacaa cctgctggag tgtggggacg
     1681 acgtggtgat cggggccggc gtctatctct cggggcacac ggtggagcgc ggcgtcgtca
     1741 ggacggcgcc ggtgcgactc ggtccgggcg tgaccatcgg cgtgggtagc gtcatcggta
     1801 tcggggtgag tatcggcgcg cacacccagg tgggagcgct gagcgtcgtg cccaagcacc
     1861 gcacgctgga gcccgacgcc gtgtacggcg gcgtcccggt gcgacgtctc gatgtgcccg
     1921 aggtgatcga gactgcctag accggtaccg ccagcagcgc gcagcacgcg cggcgcagca
     1981 accgcgcagc gaccgtggtg accgccccgg ggtcgccgta gttcatccca cggcggtagc
     2041 cgaagacgag caactcggca tcctcggcac ggcactcggc caggatctcc gaggtcacgt
     2101 cgccctgccg cacgatcgtc tcgcacgccg cgacgacgcc tccgctttgg ccgctccctt
     2161 cctgctccac gtggcgcaac caggatcgcc gctccccgtg tgccagcacg cccgcctccg
     2221 ccggctccac gtgcagcgcc accagatgag ccccaaagcc gtcggccgcc gtctgcgccg
     2281 cttcgagcac gtccctcgca tgcgggctgt cgtccacgca cgccaggact cggcgatacg
     2341 gccgatgcgc cgccggggcc accaacaccg ggacgcggca ccgccgcatc gtggcggtcg
     2401 tcacattctc ggcggcccca cgtgccacct cttcgccctt gccgagcacg atgagatccg
     2461 cgccgatctc gtccgcacac cgcgccactt cgatgctggg ccaaccgaac gctgcgcggt
     2521 aggacgcgct gcgagcggga agtaccgccg gaccgcagag cgcctgcagc cgctcgatcg
     2581 cgcgggccgc cgccagctcc gccggagagc cgctcttggc gcgccgcagc ccttcgacct
     2641 cgtcggcgcg gaccagctcc catggatccc gcagcaccgt cagcacggtc aactcggcgt
     2701 ggaacgccgc cgccacctcg gcggcagtga gcaccgcttt cggactgggg gtgtagagac
     2761 tgacggcacc gagcacccgg cgaaccttca ccacaactcc gctcctttcc cttgggggca
     2821 acttgcggcg gtgaccctga agcacatgtg aagcgagcgt gaggcaagga agtctccgtg
     2881 gctcctcaag gttccttcac ggcgacttac tgaatgttgc agagagaagg aggaggccat
     2941 gtcctggaaa cggattctcg tcggtgtgga cgagtcgccc caggccgccg cggcggcggc
     3001 gctcgcggtc cgcctcgcca aggcgctgcg tggcgaatgc attcctgtcc atgcggtgcg
     3061 gcaaatgtgg ctcgcattcg ccgaggagga gatcgtcgac cgatccgcgg agctgcaggc
     3121 ggcgctgatc gacgggtcgc gccgtaaggt cgccgcggca ctgcaggggc acatccccga
     3181 agagacgctc cggcggctga tcgtccgccc cggatctccg gccgccgtgc tgcgcgacgt
     3241 cgcccgcgac gtcgaggcgg atgcggtggt gctgggcggc aagcaccacc tcgccctgcg
     3301 tcgctgggcc ggtgggagca cagcccacaa ctgtgtgcgc accatcgggc tccccgtgct
     3361 ggtggcgggg ccgcgagccg agaccggcgg cgcgccgttc ggacggatcc tgggggcggt
     3421 cgatctgtcg gcggccgcgg ccccggtcgc agcggccgcg cgcgagctcg cggagggcct
     3481 gcgcgcggag ctgcgcgccc tctgcgtcat cgagcccccg gtcccgctcc cggatgtgac
     3541 gtccgtcctg acgccgtcgc agtattcccg tctggccgag cacgcgctga cgcgccgggt
     3601 gtggccgctg ttccgcggca gcgaggcgaa gaccgtcgca cgccagggcc cggtgatgga
     3661 aaccatccgg gaggagagcg cggcgtggaa cgccgacctg ctcgtcgtgg gctcgcacgg
     3721 gaaggggtgg ggggagcggc tgatcctggg gagcgccacg gaacggctgc tcgaagacct
     3781 gccgacgtcg ttgctggtcg ttccggtgca cgcacccgag gcgacgcgga agccgacgcg
     3841 cccgccggcc cgccgggccg tcaagaagcc cgtgcgcgcg ggaagtcacg cgtgatgccc
     3901 gacgcgcaga acgtcgctgt tgcggcgcgg ctcgaggagc tggccgcgct gctcgagcgc
     3961 cagggcgcga acccgttccg ggtggaggcc taccgtaatg ccgcggacca ggtgcgcagg
     4021 ctcgagcggc ccgccggaga catcctgcgc agcgagggca gcggcggact ggagcgcctg
     4081 ccggccatcg ggacgacgat cgcgagggcg atccgtgacc tcgtggagac cggccgcctg
     4141 ccgatgctgg accgcctgcg cggagagagc gacgcggtca cgctgctgtc ctccgtcccg
     4201 ggcatcggcc ggactctggc cgagcggctg catcacgatc atggcatcga cacgctcgag
     4261 caactcgagg ccgcggcgca cgacgggcgg ctggccgcga tccggtcgtt cggcgagaag
     4321 cggctgctcg gcgtgcgcga ggctctgggc gcgcgcctcg ggcgcgtgcc gcgcagcgcg
     4381 gtggtgagcg ccgagcccgc ggtcgacgaa ctgttgcgcg tggaccggga gtaccgcacc
     4441 cgggcggcgg ccggaacgct ccgcacgatc gcgccgcggc gcttcaaccc gcggcgtgag
     4501 gcgtggctgc cgattctcca cacgatgcag ggggaacggc actacacggc gctgttctcc
     4561 aacaccgcgc gggcgcacga cctcggcaag acccgcgagt gggtggtgat ctacgtggat
     4621 gggggcaggg gcgagcggca gtacacggtc gtcaccgcgt cccgtggacc gatgaagggc
     4681 aaacgggtgg tgcgcgggcg tgaggtcgag tgccaggccg tctacgggga gcgtgcggcg
     4741 acgcgcacac cgtcggagag cttgtccgtc ggatgacgga tcaccacgtc gcccgcggtg
     4801 agcccgccgg tgatttccac gtcgaacgga ttgcggtggc cgacctgaac ggcccgtgag
     4861 cgagcccgtc catcccggac gaggaatacg cgccactggt cccgatcctg gaacagagcg
     4921 ctggacggca ctttgagcgc ggtcccctcc cacagcacga cctgcgtctc gacccggtag
     4981 ccatcgccca gctggggcgg tgtctcgtcc agattggcga tcacgttcac gcgctgctcc
     5041 tcgacgccaa gggccgatac cttggtgaac ccggatggct cgacgcggcg cacgtgggcc
     5101 tccagcgggc gctcgcctcc ccagtcggcg accagcatgc ggtctccggg gcgcaccttg
     5161 accgcgtcct cggacagcag gtccaccacg atctcgagcc gcgtggggtc gcccagctcg
     5221 agcagcggcg tccccgccgc gacgacgcgc tcgctccgct ccgggatgcg gagcacacgc
     5281 ccgcgcaccg gcgagacgag cgccagcgcc gagccgcctc cgccggcgat gacggcgcgc
     5341 gcctgctcga cgtcgtgcgc ggccgcctga gccgtgaagt ccgccgcctg gaagcgccgc
     5401 accgcggtgg tctccgccaa cgccgcttcc tcgcgctgcg cgggggccag caggttcttc
     5461 gcgaacagcg actccgcccg tgcccggttg cgggtcgcct gatcgagcgc ggcgcgagcc
     5521 tccgacacgg cggccgccgc ggcccgctgt gcgtcctcgg cgccgtcgag tcgggcgacc
     5581 gcctgctcgc gggagcgtgg gtcgagcggc gcggccgaga gccgggccac gaccatgccg
     5641 cgcgtcacgg gggcaccctc ttcgagcgcg atccgcgcca cccgacccgc cgccggtgcc
     5701 acgacgacgt aacggtcccg tacccgcgtg cggccctctt cgtcgatggt gacccgcagc
     5761 gctccccgtt ccaccgccgc gacctcgacc ggaatcgcgg tcggccggaa cgtgtacccg
     5821 agaaacgccg ccgcggcgac gccggcgcct ccccacagca ttgcccggcg attgaggttc
     5881 atcgcgtcaa tctcccgtct tcaggacgcc cacgagatcg aggcggtcga tccggcgccg
     5941 cacgatcagc gcggaggcga gcgcagccag caggatcacg atgaccgcat agccgtaggt
     6001 ggcggggtgc aacacgaacg gcaaccggta caactcggtg tcgaacgcca tcacgatcag
     6061 cgcggcaccg ccgtagccga acacgaagcc gagtggcagc gcggccagga ccagcaccgc
     6121 ctgttcgtcg agcagaatgc gggacgtctc ggcgcgggta aatcccagta cccggaggct
     6181 cgccagctcg cgggcgcgct ccgagagggc gatgcgcgcg gtgttgtaca cgacaccgaa
     6241 cgcgatcagg accgcgaagg ccacgatgta cgtcgtgacg atgccgtgac tccgcgccag
     6301 cgtctcgtcg aagctctgga cggtaggctc gcggatcgag acggccgcga ccgcgggcag
     6361 gtgtttcagg gcgtcgtaca gcgccggcgc cgctcgcgcg tccaccgcca gcgccgcacc
     6421 ggagaccgtc ccgccctcgc gcagcagccg gtgcaacccg cccgcgtcca tgtaggcgag
     6481 ggtgcccatc ggttcgtgca ccaggccgac gaccgggacg acgagggtcg gacgcgcgcc
     6541 ctcgagcact tcgacggtga cggggtcgcc gggcgccaca cccagcacct gcgccagctt
     6601 gtcggtcagc aggactccct cggcggggag ggagcgacgc cgcccgtcga ggtcggccac
     6661 ctgtcgcagc tcggcgtcgg gctcgagtcc gaggatcacc gtacgccgcg accggtggcc
     6721 ggcgatcagg cgcactccca cgtcgcggaa cggctcggcc cggaggacgc cgggaagatg
     6781 ccgcacctcg tagcgcacgc gcgccggccg cggctcggtg aaggagatgc tcacggcctg
     6841 gcgctcgcgc acgtcgaact cgaaatccgc caacgcctcg accgcatcga tggtgaaccg
     6901 gcccagcacg atgatgccgc aggccaggcc gatcccggct gcggtcacga tcgcccggcc
     6961 cggacggcgc tccagcgtgc gcagcaccat ccggcccacg ggcccgaaga ggcggtggag
     7021 cccgagcgcc tcgaaggcgc cgcgtcggta gcggggtggg ggttcggggc gcatcgcttc
     7081 cgcgggcggc aggcgcacgg cacggcccac ggcgctcaac gcgccgagca gcccggagcc
     7141 ggcgctcacc agggtggcga gcgccagcac gccccagccg gcggtgaagt gcagcaccgg
     7201 gaagcggtag taggccgcgt acagcacggt cagccgcccc cccagccaca tgccgaacgc
     7261 cgcccccacc gccgcgccga gcagctcgac gcccaccacc agtttggcat agtgcaggcc
     7321 gacgtcgacg tcgctccgcc caaaggcctt gagcaccccg atctgcggcc gctgcgtggc
     7381 caccaggcgc gcgagcacga tgtgcaccag gaacgccgcg acgcccagga agatcacggg
     7441 gacgatcgcc ccgctgacgc gcagctgggc gatctcgtcg gtgatgaagc ggttggactg
     7501 gtgatcggcg cggccgatgg cacccagccc accgtagggt gcgagcaggc ggtcgaggct
     7561 cacgcggacc tcgcgctcat cggcgccggc cgtgaggcgc agggagacgt cgttgaacgc
     7621 gccctgcatg tcgaacgcgg tggtcagcgc ctcgtggccc atccagaaga cgccgtagcg
     7681 cttgtcgtcg gggaagaacc agcccgggga gatctgatac acgaattcgg gggagagcgc
     7741 gatgccgacg atgcgcagcc gctgccagcg gccgttgatg atggcgccca gggtgtctcc
     7801 gacctgcagc cggttcgcct gagcgaactg ctcgctcgcg acgacctcgt cggaccgccc
     7861 gggggcgatg tagcggccct ggcgcagatg gaggtcgttg agcatgggaa cgcggcgctc
     7921 gggaaccgag acgaggcgtc ccgtcgccgg ctcggcgaga ccggggacgg cgagcgtcac
     7981 gtgcacgacg acgcgggact gcacggcggc cacgcccggc agcgccgcga tcttcgcggc
     8041 gagcggatcg ggagcgcgtc gcagcgaggc gaacacgtcg gcgaagcggt agtcgcggta
     8101 gtaggcgtcc agcgagccct tcaacgagtc gtaggcggtg cggatcgtga cgatgctcgc
     8161 cacgccgcag gccacgatca gcgccaccgc ggccatctgg ccgcgcaggt gccagagatc
     8221 gcgcagcgcc ttgcggtcga gtgccgtcac cattgcagcg tgtccaccga cgcccggacg
     8281 ggattgcggt cgatcgacgc gatctggccg ctgtgcatgt ggaccacgcg gtcgcccatg
     8341 cgtccgatcg cgacattgtg cgtgatgatc gccaccgtgg tgcgcaggtc gcgattcacg
     8401 cgctccagca cctcgagcac gagcttgccg gtctggaaat cgagtgcgcc ggtcggctcg
     8461 tcgcacagca gcacatcggg ctgcttggcg atggcgcgcg caatcgccac ccgctgctgc
     8521 tcgccgcccg acagctgcgc cgggaagtgg tcgaggcgct ctcccaggcc gacgagctcg
     8581 agcgcgcgca ccgggttgag cggccggggg gccgtctcgc acacgagctg cacgttttcg
     8641 cgcgccgtca ggctggggat caggttgtag aactgaaaca cgaacccgac gtgctcgcgg
     8701 cggaaccggg tgcgctcccg ctcaccggcc gcggtcaggt cgtgatcgaa gaaccggacc
     8761 gtgcccgacg tcggaacgtc caggcccccc aggatattga gcagggtcga cttgccgctg
     8821 cccgagggac cgagaatgac cacgaactcg ccgcggtaca gctcgaggga gagagagcgc
     8881 agcgcgtgta cttcgacctc acccagccgg tacaccttgg agaggtcgtg cgcgcgaaac
     8941 acgggctctg ccggagaggg tccccccgtc gccccgacgg aggaaacgaa aggtgcaggg
     9001 gccgccatca tggttccatc gtagcgggcg cgggagcgcg cctccgtgaa ggttccttga
     9061 agcccgcttc acgcccgcta gaccgtgagg cggatgaccc cttcgtcgag ttcccgccgg
     9121 accgggacgc cggcgccggc caccagctcc agcatgcgcg tgttctgcgg cagcacctcg
     9181 gccgtgatct ccgcgatgcc gtgatcgtgg gcgacgccga gcaggtgttt cagcagcacc
     9241 gtgccgatgc cgcggttctg aaacgtgtct tcgacgatga taccgagctc ggcccgaacg
     9301 ggctggtcgg gctgtgccgg caggacatcg tagcgcgcga cgccgacgat gggggctcgg
     9361 cggtcggggg aaagtgccgc gaccagcgcc acgcggcggt cgtagtccag ctcggtcaga
     9421 taggtgaggt cgcgcgccga caggaccggc ttgtactcga aatagcggag gtacaccgac
     9481 tcggggctga gacggccgtg gaaccgggcc aggcgttccc gatcgtcggg ctggatcgcc
     9541 cggatccaga ccggggcgcc gtcccgcagg aggtctgccg cagcgtagtg ccgcgcttcc
     9601 atggggacac agggtagcgg gctctcggag gccgggccag acccgctgta tcgcggctct
     9661 ggagcgcagg ttagaatacg ttgtgcctgc caagcggccg tctcgttccg agacaccccc
     9721 gcgcccagcc aggcccagtg gcgacagccg ccgcctggag gaccggttcc ggcacctggt
     9781 cgcggcgagc gcacggattt tttcccagca cacccttccc gcggtcctgc aggaagtcgt
     9841 cgatgccgcc cgcggtatcg tcggcgcgcg ctatgcggcg ctcg
//
LOCUS       DBNGZW010000669         1418 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1819483, whole genome shotgun sequence.
ACCESSION   DBNGZW010000669 DBNGZW010000000
VERSION     DBNGZW010000669.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1418)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1819483"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..780)
                     /locus_tag="AC16JC_15780"
     CDS             complement(<1..780)
                     /locus_tag="AC16JC_15780"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR5166209.1"
                     /translation="MNALRTAAFLLSAVTPLAAQRAPTGSVVGTVRDSGGAAVAGAQV
                     RVLPTGAGALTDSAGRYRLEGVPAGSVTLQAARIGFYPLQLVVLVAADDSVRADFQLR
                     ANPLLIASVIVTAAKRSQLVDQAVTSVALVGGQQIARRAVNTIDEAVDKAPGVQMIAG
                     QVNLRGSSGFVEGFGSRVLLLVDGVPANQGDRGGINWDLVPVDQVARVEVVKGAGSAL
                     YGSSAFGGVVNLITDEPPLGFHARLRATGGVFADPPHAAWGF"
     gene            complement(777..1286)
                     /locus_tag="AC16JC_15785"
     CDS             complement(777..1286)
                     /locus_tag="AC16JC_15785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166210.1"
                     /translation="MRPRRAILFVLLVVIGCDEGLTPRPTAAACPTGFVGVCGTVTFR
                     GALPDSTEAVYIVAYATFPTTQDDLFLFLPVPPPALPLDSVGTTGGQHYQLALPNGSY
                     AWVLAAWKKVGLISPANADSLLREAGYYRDPSDTTRPGTVVVSGARTDAIDFVVDFTN
                     MHPVSFYFP"
BASE COUNT          243 a          483 c          505 g          187 t
ORIGIN      
        1 gaacccccac gcggcgtggg gcggatccgc gaacacgccg ccagtggcgc gcagccgggc
       61 gtggaatccg agcggcggct cgtccgtgat gagattcacc acgccgccga acgcggagga
      121 gccatacagc gccgagcctg cgcctttcac cacctcgacc cgcgcgacct gatccaccgg
      181 gaccaggtcc cagttgatgc cgccgcggtc gccctggttg gcggggacgc cgtccacgag
      241 caagagcacc cggctcccga agccctccac gaagcccgag gagccccgca ggttgacctg
      301 accggcgatc atctggaccc ccggtgcctt gtccacggcc tcatcgatcg tgttgacggc
      361 acggcgcgcg atctgctgcc cgccgacgag cgcgacgctg gtgaccgcct ggtccacgag
      421 ctgcgagcgc ttggccgcgg tgacgatgac cgaggcaatg agcagcggat tcgcccgcag
      481 ctggaaatcg gcgcgtaccg agtcgtctgc ggcgaccagc acgacaagct ggaggggata
      541 gaatccgatc cgggccgcct ggagcgtgac gctgcccgcc ggcacgccct ccagacgata
      601 gcggccggcg gagtcggtga gcgcccccgc accggtcggg agcacccgga cctgcgcgcc
      661 ggcgacggcg gcgccgccgg agtcgcggac cgtgccgacc acgctgcccg tcggcgcgcg
      721 ctgggcggcc agcggcgtca cggcgctcag caggaaggcc gcggtgcgaa gcgcgttcac
      781 ggaaagtaga aggacacagg atgcatgttc gtgaaatcca ccacgaagtc gatcgcgtcg
      841 gttctcgcac cactcaccac gaccgttccg ggtcgggtcg tgtcggacgg atcgcggtag
      901 tagcccgctt cgcgcagcag gctgtccgcg ttcgccgggg agatgagccc gactttcttc
      961 cacgcggcca gcacccaggc gtaggagccg ttggggagcg cgagctggta gtgctgtcca
     1021 cccgtcgtgc cgacgctgtc gaggggcagg gcaggcgggg gcacgggcag gaacagaaag
     1081 aggtcgtcct gggtcgtggg gaacgtggcg taggcgacga tatagacggc ctcggtcgaa
     1141 tcgggcaggg cgccgcgaaa ggtcacggtg ccgcagacgc ccacgaagcc ggtcgggcac
     1201 gcggccgccg tcgggcgcgg cgtgagtcct tcgtcgcagc cgatcaccac cagcaggacg
     1261 aagagaatgg ctcgccgcgg gcgcatgagt tcacaatctg cgcatgtgcg acgcgtcgtc
     1321 aacttgaccc cctcccggcc ggggaataac gtagtcgcca cgatgccgat ctacgaatat
     1381 cgctgcacca agtgccgcaa gcggttcagc gagtcgat
//
LOCUS       DBNGZW010000670         2701 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_199695, whole genome shotgun sequence.
ACCESSION   DBNGZW010000670 DBNGZW010000000
VERSION     DBNGZW010000670.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_199695"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..415
                     /locus_tag="AC16JC_15790"
     CDS             <1..415
                     /locus_tag="AC16JC_15790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF014787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR5166211.1"
                     /translation="RLIEGPYPNYEQVIPRENDKFATADKQAMSAALRRMSVVASDQT
                     HRIRLGFSGGLVKFSVQTPDLGEAQDELAVTYEGEPFEIGFNATYLLEILKYMPTDEV
                     RFTFKAPERAATIEPVGWNDPASYLALVMPLRLVD"
     gene            complement(376..1281)
                     /locus_tag="AC16JC_15795"
     CDS             complement(376..1281)
                     /locus_tag="AC16JC_15795"
                     /inference="COORDINATES: protein motif:HMM:NF013106.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HIR5166212.1"
                     /translation="MVIGNKRTAARRIAWGLLIAALGWLVGVWPPPVWWGDHWPVETA
                     MMRRRGAPVSYQPVPLEQFPSVLQRMVIIGEDSRFRTHHGLDFEEIRDALGLDQQAGF
                     GTTMRTIWRRRDRLRGASTITQQLAKNLYLSPSRNPLRKVKEALTALRLEWMLSKDRI
                     LELYLNLVELGPGVWGVPAASAQYFGVTPAQLSEAQAAALAATLPFPLSSNPVYRSGR
                     MLHRRDLILARYHGVDVYIPPEEELDTVPIPPFLPPIVPPVLDTLLRDTLTVPVESTP
                     PDTLTDSTRDTTPQSTRRKGMTSAR"
     gene            complement(1314..2135)
                     /locus_tag="AC16JC_15800"
     CDS             complement(1314..2135)
                     /locus_tag="AC16JC_15800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166213.1"
                     /translation="MTRRVLGVSLALALGGAMRDAARAQVAPNRATAYLPATDVGDVR
                     ALWVNPGGLAARPEAAVLLDLTVRAPGSGGRLAQVSAGFNARGLAFGYQRDNFTNGIH
                     GHTYRFGLGGAARSFTFGAALALYRGATSGTGWDFGILYDWRPELTIGGVVRNVGKPT
                     VRGVTLEPTYVPALTVRPLASRLALTAQAEAISSTVLGFAFEARAVLPAPSLSFLLRV
                     DTDRSLHRQMLVLGFAIGRANEVGLVGSSLGGHAQQNVLSAYGVATRAPGTRRGN"
     gene            complement(2132..>2701)
                     /locus_tag="AC16JC_15805"
     CDS             complement(2132..>2701)
                     /locus_tag="AC16JC_15805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166214.1"
                     /translation="VVDSASRLPPQIDLAAARGFRVTGRLVPTGEFISGPCDTAAAAA
                     SLGSLLPRFRNFFPRLPAAGVVPQTSWTDSTKAFDPATCSGGTALTTRSENHRAATTW
                     EDRDGIRALRLETTATYQFNGSGDQGGSPFTLSGSGVGTGVQFLSADGRYVGGEVRDS
                     STLTIDLSVQGISIPRRQLAHTTVTVLPR"
BASE COUNT          443 a          916 c          968 g          374 t
ORIGIN      
        1 ccgcctgatc gagggaccgt atcccaacta cgagcaggtg atcccccgcg agaacgacaa
       61 gttcgcgacg gcggacaagc aggcgatgag cgcggcgctg cgccgcatga gcgtggtggc
      121 gagcgaccag acgcaccgca tccgtctcgg gttctccggc ggtctcgtga aattctcggt
      181 gcagaccccg gacctgggcg aggcccagga cgagctcgcc gtcacctacg agggcgagcc
      241 gttcgagatc ggcttcaatg ccacgtacct gctcgaaatc ctcaagtaca tgccgaccga
      301 cgaagtgcgc ttcacgttca aggctccgga gcgcgcggcc acgatcgagc ccgtagggtg
      361 gaacgacccg gcgagctacc tggcgctggt catgcccttg cgcctggtcg actgaggcgt
      421 ggtatcacgc gtgctgtcgg tgagcgtgtc cggcggcgtg ctctccaccg gcaccgtcaa
      481 cgtgtcccgc agcagcgtgt cgagcaccgg gggaacgatg ggcgggagga agggcggtat
      541 ggggaccgta tcgagctcct cctccggggg gatgtagaca tccacgccgt ggtagcgggc
      601 gaggatcagg tcgcggcggt gcagcatgcg gccggagcgg tacacggggt tggacgacaa
      661 cgggaacggg agggtcgcgg cgagcgccgc cgcttgggcc tcactcagtt gcgccggcgt
      721 gacgccgaag tactgggcgc tcgctgcggg cacgccccac acgcccggtc cgagctccac
      781 caggttcagg tacaactcga ggatcctgtc tttcgagagc atccactcga gacgcaacgc
      841 ggtcagcgcc tccttcacct tgcgcagcgg gttgcgcgag ggtgacagat agaggttctt
      901 cgccagctgc tgggtgatgg tgctggcgcc ccgcagccga tcacgccgcc tccagatggt
      961 ccgcatggtc gttccgaacc ccgcctgctg gtccagcccg agcgcgtcgc ggatctcctc
     1021 gaagtcgagg ccgtggtgcg tgcggaaacg cgaatcctct ccgatgatca ccatgcgctg
     1081 caggacggac ggaaactgct cgaggggcac gggctgatag ctgacgggag cgccgcggcg
     1141 gcgcatcatg gcggtctcca cgggccaatg gtcgccccac cacaccggcg gcggccacac
     1201 gccgacgagc cacccgagcg cggcgatcag gagtccccag gcgatccgcc gtgcggccgt
     1261 acgtttattc ccgatgacca tgtctcaacg atcgcgcgcc ggactcgcgg ttcctagttg
     1321 ccccgccggg tcccgggagc gcgggtcgcg acgccatagg cgctgagcac gttctgctgc
     1381 gcgtgcccgc ccagggacga gcccaccagg ccgacctcgt tggcgcggcc gatcgcgaac
     1441 ccgagcacca gcatttgacg atgcagcgat cgatcggtat cgacccggag caggaaggag
     1501 agggagggag ccggcaacac cgcgcgagcc tcgaacgcga atcccaggac cgtggacgag
     1561 atcgcctcgg cctgcgccgt gagcgcgagc cggctcgcca gcgggcggac ggtgagcgcg
     1621 gggacatacg tgggctcgag cgtcacgccg cggacggtgg gcttgccgac gttgcgcacg
     1681 acgccgccga tcgtcagctc cggccgccaa tcatagagga tcccgaagtc ccatccggtt
     1741 ccgctcgtcg ccccgcggta cagcgccagt gccgccccga aggtgaagct ccgcgcggcg
     1801 ccgcccaatc cgaagcggta ggtgtgaccg tggatcccgt tcgtgaaatt gtcgcgctgg
     1861 tagccgaaag cgaggccccg ggcgttgaat ccggcgctga cctgggccag gcggccgccg
     1921 gagcccggcg cccggaccgt gaggtcgagc agcacggccg cctccggtcg ggcggcgagg
     1981 cccccgggat tcacccacag cgcgcgcacg tcgccgacgt cggtcgcggg cagatacgcg
     2041 gtggcccgat tcggggccac ctgcgcacgg gcggcgtcgc gcatggcgcc gccgagggcg
     2101 agcgccaggc tcacgcccag gacgcgccgc gtcaccgcgg gagcacggtg acggtggtat
     2161 gcgccagctg gcgtcgcggg atggaaatgc cctgcacgct caggtcaatg gtcagggtgc
     2221 tcgagtcgcg cacttccccg ccgacgtagc ggccgtcggc cgacaggaac tgcacgccgg
     2281 tccccacgcc cgagccgctc aaggtgaagg gagagccgcc ctggtctccg gaaccgttga
     2341 actggtaggt cgccgtggtc tccagccgca acgcacgaat cccgtcacga tcttcccagg
     2401 tggtggcggc gcggtgattc tcggaccgcg tggtgagcgc cgtgcccccc gagcaggtgg
     2461 ccgggtcgaa cgccttggtc gagtcggtcc aggaggtctg aggtaccaca ccggcggcgg
     2521 gcagccgcgg aaagaaattg cggaaccggg gcagcaaact gcccaggctc gcggcggccg
     2581 cagcggtatc gcacggcccg ctgatgaact cacccgtggg gacgagccgt ccggtcaccc
     2641 ggaatccccg agcggcagcg agatcgatct gtggcgggag tcgcgacgcg gaatccacga
     2701 c
//
LOCUS       DBNGZW010000671        10886 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_948590, whole genome shotgun sequence.
ACCESSION   DBNGZW010000671 DBNGZW010000000
VERSION     DBNGZW010000671.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 10886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10886
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_948590"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /gene="xseA"
                     /locus_tag="AC16JC_15810"
     CDS             <1..889
                     /gene="xseA"
                     /locus_tag="AC16JC_15810"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HIR5166215.1"
                     /translation="FGSPTVWEERGEFRLTVRHLLSTEAGGLWQVAFEKAKAALQRDG
                     LLDPARKRPMPPFPRRIAVVTSADGAALRDIVAVLERRWPADVVLVPTRVQGDGAERE
                     VCAALALLARIPRLDLAIVGRGGGSKEDLWTFNSERVARAVAAVPVPVISAVGHETDV
                     TLCDLVADVRAPTPSAAAAAATPDREELQDRLDRLSRLLARALLAPTRHAVERLERAG
                     DRMTAGIGRQVDRARARLSESGARLDALSPLRVLERGYAVARGETGGVLRRVAQFTPA
                     LRFRLRVQDGEVAARVGEA"
     gene            889..1122
                     /gene="xseB"
                     /locus_tag="AC16JC_15815"
     CDS             889..1122
                     /gene="xseB"
                     /locus_tag="AC16JC_15815"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HIR5166216.1"
                     /translation="MAESPASGGFAEDLERLEQIVRRLEAEELDLDEALRLFEEGVQR
                     LRAARERLGAAELRVRQVIADQAGKLRLEDVDR"
     gene            1103..2029
                     /locus_tag="AC16JC_15820"
     CDS             1103..2029
                     /locus_tag="AC16JC_15820"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011465153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004659 - prenyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HIR5166217.1"
                     /translation="MKTSIVDRALDAYLADTRARVDAVLERWCTRAESFWPAPVPAAV
                     RYSVLSEGKRLRPTLVFAAYEAMGGDAGAEGIAELAAAVEVVHAYSLIHDDLPCMDND
                     DLRRGRATVHRAFDVATATSAGFHMVGLAARVLVSGLDALDLPADRRRAVALELFRAA
                     GGGGMIGGQVLDLEAEGRHISLEALREVHQRKTGALIAAACVIGGLAAGGVSPGEHLA
                     ALRAYGEAIGLAFQIADDVLDATATSAQLGKTAGRDAALAKSTFVSVLGVGAARAEAE
                     RLAAQAVDRLRHAALVSPTLVALAHYIVTRPN"
     gene            2026..3936
                     /gene="dxs"
                     /locus_tag="AC16JC_15825"
     CDS             2026..3936
                     /gene="dxs"
                     /locus_tag="AC16JC_15825"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HIR5166218.1"
                     /translation="MTAVTLLESIRDPGDVKRLPREQLQPLADEVRRRLIDVVSQTGG
                     HIGAGLGVVELTVALCHVFDSPRDKIVWDVGHQGYPWKILTGRNDRLPTLRQEGGLSG
                     FLRRDESPHDQFGAGHAGTALSAALGMATARDLAAADYKVVAVVGDGAMTCGLSYEAM
                     NNAGHSDRDLIVVLNDNGMSIAPNVGAMSKYLGSIIASPVGVRWRERVKHAIEKASHL
                     VGGPRLVEFAKTVEESVKNLWSPGMLFEEMGFRYFGPIAGHDLPHLVSTFEIVRGLKG
                     PRLVHVLTEKGKGFPLPEPDVEKYHARAPYDPVTGVLKPVAPGAPQWTKIFGEALTQL
                     AGEYPQLVAITAAMPSGTGTNLFQKKWPARFFDVGIAEAHAVTFAAGLATQGIRPVVA
                     IYSTFLQRAYDSIVHDVAIQSLPVIFCLDRAGMVGEDGQTHMGLYDIPYLLALPNMTV
                     TAPKDGAELIGLLRCALAHQAGPFALRYPRDKAPGEAPLAAEVTPVPYGTWEVLRRGR
                     ECALLAVGVMCRPALEAADLLARDGFDVSVVNCRFLKPMDHAMLDTLTRDHKLLIPIE
                     DGAVTNGFGATLAGQLQGTASEVRVVPIGAPDRTYEHAPRARQLEAAGLSAKGIAARV
                     RALAAEESLAPR"
     gene            3933..4817
                     /locus_tag="AC16JC_15830"
     CDS             3933..4817
                     /locus_tag="AC16JC_15830"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF039482.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0006741 - NADP+ biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD+ metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HIR5166219.1"
                     /translation="MNVGVVGNPSYHDLRSLLARLTAAAPRLGFTLYSEERLLDYWPD
                     QRPPVFHADTPLDCLLTFGGDGTLLRGARLLDGASTPILGVNVGRVGFLATAPAHNLD
                     WVLDAAARKAYTIEPRLALVATVEDRQGARRPQPVALNDIVVHKGGVARVLRFRVSVD
                     GEEVGTYSADGIIVATPTGSTAYSLSAGGPIVVHGVDALVVTAICPHTMAVRPLVLPS
                     STTVMIEPVPPWSDTEEILLSLDGQLSRALQPGERLLVRRAERPVLLGRLGPEGFFAR
                     MRRKLNWGDLADRERREC"
     gene            4811..6535
                     /gene="recN"
                     /locus_tag="AC16JC_15835"
     CDS             4811..6535
                     /gene="recN"
                     /locus_tag="AC16JC_15835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HIR5166220.1"
                     /translation="MLTELRVRDLAVIADVTLRLGPGLNVLSGETGAGKSMLVDALAL
                     LLGERASAEAVRPGAARTVIEGAFELSAGTARRLAAPLAALGVEVEEGRLILKREVSG
                     EGRSRAWVNGSPATVGVLAQLGGLLVDLHGQHDTQSLLHAETQRDLLDAYAGAEVERV
                     AVRDAHARVRALEEREAELRGKQEEIRRKADYLRHVVEEISRAAPKVGEDEALEGEAR
                     RLAHGDELGRLSRDLEQALDAGGLARAAKLLAALERLDPSTGSWRELLDTAFANVNEL
                     AAAAREYGAGIDADPARLEEIERRRDTLFRLLQKYGPAIPDVLAARDAAARELDLLDN
                     ADLDLRDLSAQRDAAVQEFTRACAALSEKRRQGAQRLARALTARLTPLGMPGGRFTVD
                     LGSYTAARADGAEGVAFLVRLNEGMEARPLARVASGGELSRVMLALKVVLAHHDAVPT
                     LVFDEVDQGIGGEAAAQVGAALADVVREGAEHERQALVITHLPQIAARADRQLAVAKR
                     DRGGIATSDVRLVEGEERIREIARMLGDHDSATARRHAEDLLRTAESSAASRSETPSR
                     PAPGRPRR"
     gene            complement(6459..8003)
                     /locus_tag="AC16JC_15840"
     CDS             complement(6459..8003)
                     /locus_tag="AC16JC_15840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166221.1"
                     /translation="MRQRPVFSLSIAAAAIVCLPVGRLTAQAVERTDTPRRGALRVTF
                     DPRIMTWEEVFTPGGRQPVGAAFAGDSLTPWQPALALAQENVRAATGLAGYVARLGGS
                     LLAIRSERRVTPIQLEYGITNRLSLGATIPIVRVEVRERYRFSPEGSNLGLLPDRARY
                     TDFFGHLGTALDQLGVNINAGAYGSPGSPTYLRAQALLAQGRAFASACSTAVFDTTAR
                     FLPTEGSDAGRSLTGIVLGLQHALADTFNVATFSRDTFFLPVVPVPGEAAAGILADSL
                     SQLGLTPYGPGTPRRLRYFPGDAEVAAKYRFFSGDSYAAAAALLVRLPTGHQDSPFDP
                     FDLSTGDHQTDVEGRLTAELTLMHRLWLNLSIRGARQLPGERERLIGPVDQPFLSRST
                     GKRLRWDPGDYVAVDVAPMFRFSRLFAIGVTFGYYAQQRDRYAFLAPQDSADVATAVG
                     GPVSASVLEPGTGIRQARLGVAATFAGPRLEGGFSVERTISGAGGPVPVATVFRIVMR
                     QTILLF"
     gene            complement(8071..9303)
                     /locus_tag="AC16JC_15845"
     CDS             complement(8071..9303)
                     /locus_tag="AC16JC_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014065857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR5166222.1"
                     /translation="MTRTTKFLGAALTGVLLAVACHNDELFRPANTQPIDPLFTRYVA
                     MGNSLTAGFQSGGINDSTQLLAYPNLLSKQMHTPFFMPLLNRPGCPPPIDTLFRASGT
                     PHRVGGGTASTCALRQFQPIPPPYISDVAVPAAEVIDGTSNLDTASNANPLTTLILGG
                     LTQSQAMQRANPTFVTVWLGNNDVLGAAISAANAGDSTKVTDSTLFANRYKAVLDAID
                     ATPASGKGVLIGVANVTLIPFFSQGQTYFAIKNTTANFPPNFIVGPNCAPSALGGKGD
                     SVLVPFQFGLGLIGLAAANPAGTFTLSCTETQTVQPAELVRLVRAVNQYNATIAAQAT
                     GRGYAYVDPNALFTALPPGSIPPFPTTTGPASVTAPFGTYFSRDGIHPTGLADKLVAN
                     ALIQAINAKYGTSIAAIP"
     gene            complement(9322..10668)
                     /locus_tag="AC16JC_15850"
     CDS             complement(9322..10668)
                     /locus_tag="AC16JC_15850"
                     /inference="COORDINATES: protein motif:HMM:NF015316.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpP1/FadL family transporter"
                     /protein_id="HIR5166223.1"
                     /translation="MRRFTWLLLTGVPSLLGAQGFGVYEHNTCTMGRGGVSAARPCAD
                     GSAIFFNPAGLGGLSGGHVSAGVTLIHAQGGFTDDLFAKKTDLANPTIPVPNGYLTYA
                     VSPKLTVGVGVYAPYGLETKWPIAGFDGRFMGYNTKIHSIYVQPTLGYQVGGWLKVGV
                     GVAYIRSTLELHERLDLATQSPAPGITFGALGVLPGTDFANADLSASGNGFAVNFGGI
                     IKLTDRLSIGGHWITRKTISYDGDAVFDQIPTGLTLPPNNPLGLPGGTPVDALLAAEF
                     AAGGPLANGGASTSITMPPQGTFGFAYQMRDNWSVMADFQYVVWGWFQSVAIKFANPA
                     TPTLSLSPKNKDTYGFRIGTEYEPSAKLTLRGGYLYHTAASPVDFVTPLLPEAPRNEF
                     TAGVGYDFTPRLHGDLAYQYIAQQDRRGRVFPLPVSNTGLYEFSAHLFGAGLHFTF"
     gene            10754..>10886
                     /locus_tag="AC16JC_15855"
     CDS             10754..>10886
                     /locus_tag="AC16JC_15855"
                     /inference="COORDINATES: protein motif:HMM:NF021988.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase family protein"
                     /protein_id="HIR5166224.1"
                     /translation="MRTQAIALFHRVFGGAPGTVASAPGRVNLLGEHTDYNGGPVLPM
                     "
BASE COUNT         1624 a         3664 c         3962 g         1636 t
ORIGIN      
        1 cttcgggtcg ccgactgtgt gggaagagcg cggggagttc cggctgaccg ttcggcacct
       61 gctctccacc gaagccggag gcctgtggca ggtggccttc gagaaggcca aggcggcgct
      121 gcagcgcgac ggtctgctcg atcccgcccg caagcggccc atgccgccgt tcccgcgccg
      181 catcgccgtc gtgacgagcg ccgacggcgc cgccctgcgc gatatcgtgg ccgtcctcga
      241 gcgccgctgg ccggcggatg tggtgctggt gcccacccgg gtgcagggtg acggcgcgga
      301 gcgcgaggtg tgcgccgcgc tggccctgct ggcgaggatc cctcgcctcg accttgcgat
      361 cgtgggccgc ggcggcggct cgaaggagga cctctggacc ttcaacagcg agcgcgtcgc
      421 ccgtgccgtc gccgcggtcc cggtcccggt catctcggcc gtcggtcatg agaccgacgt
      481 gacgctgtgc gacctggtcg ccgatgtgcg cgcgcccacg ccgtcggccg ccgccgccgc
      541 cgcgacaccc gaccgggagg agctgcagga ccggctcgac cggctgtcgc gcctgctggc
      601 gcgcgccctg ctcgcgccga cccggcacgc ggtcgagcgg ctcgagcgcg ccggtgaccg
      661 gatgacggcg gggatcgggc ggcaggtgga tcgcgcccgc gcccgtttgt cggagagcgg
      721 cgcccggctc gatgcgctgt ctcccctccg ggtgctggag cggggctatg ccgtggcgcg
      781 gggcgagacc ggcggagtgc tgcgccgggt ggcgcagttc acccccgcgc tgcggttccg
      841 cctgcgggtg caggacggag aagtcgccgc gcgcgtcggc gaggcgtgat ggcggaatcg
      901 cctgcctccg gcgggttcgc cgaggacctc gagcggctgg agcagatcgt gcggcgtctc
      961 gaagccgagg agctggacct ggacgaggcg ctgcgcctgt tcgaggaggg cgtgcagcgg
     1021 ctgcgcgccg cccgcgagcg cctcggcgcg gccgagctgc gggtgcggca ggtgatcgcc
     1081 gaccaggccg ggaagttgcg ccttgaagac gtcgatcgtt gatcgcgcgt tggacgccta
     1141 cctcgccgac acccgcgcgc gcgtcgatgc cgtgctcgag cggtggtgta cgcgggccga
     1201 atcgttctgg cccgccccgg taccggccgc ggtccgctac agcgtgctga gcgaagggaa
     1261 gcgcctgcgc cccaccctcg tgttcgcggc ctacgaggcg atgggaggag acgccggcgc
     1321 ggagggcatc gccgagctcg ccgcggccgt cgaagtggtg cacgcctact cgctgatcca
     1381 cgacgatctt ccctgcatgg acaatgacga cctgcgccgg gggcgggcca cggtccaccg
     1441 ggcgttcgac gtcgcgacgg ccacgagcgc cggattccac atggtgggtc tcgcggctcg
     1501 cgtcctcgtc tccgggctcg acgccctcga cctcccggcg gaccggcggc gggccgttgc
     1561 gctcgagctg ttccgggcgg ccggcggcgg cggcatgatc ggcgggcagg tgctcgatct
     1621 cgaagcggag gggcggcaca tctcgctgga ggccctgcgg gaagtgcatc agcgcaagac
     1681 cggcgcgctc atcgccgcgg cgtgcgtgat cggcggactc gcggccggcg gcgtgtcgcc
     1741 gggcgagcac ctggccgcgc tgcgcgcgta cggggaagcg atcggactcg cgttccagat
     1801 cgccgacgac gtgctcgatg ccacggcgac gtcggcccag ctggggaaga cggcggggcg
     1861 ggatgccgcg ctcgccaaat cgacgttcgt ttcggtgctg ggcgtgggcg cagcgcgcgc
     1921 cgaggcggag cggctggccg cgcaggccgt agaccggctg cgtcacgcgg cgttagtttc
     1981 ccctacactc gtggcgctgg cgcactatat cgtgacccga cccaattgac tgccgtgacc
     2041 ctgctcgaat ccattcgcga tcccggagac gtgaaacggc tgcccaggga gcagctccag
     2101 ccgctggccg acgaggtccg tcgtcgcctg atcgacgtcg tctcccagac gggcgggcac
     2161 atcggtgccg gactgggcgt cgtcgagctc acggtcgccc tgtgtcatgt gttcgactcc
     2221 ccgcgcgaca agatcgtgtg ggatgtgggg caccagggat acccctggaa gatcctcacc
     2281 gggcgcaacg accggctgcc gacgctgcgg caggaagggg gcctctcggg cttcctgcgg
     2341 cgcgatgaga gcccgcacga ccagttcggg gcggggcacg ccgggacggc gctgtcggcc
     2401 gcgctcggca tggcgacggc acgtgatctc gccgcggccg actacaaggt cgtcgccgtg
     2461 gtgggggacg gcgccatgac ctgcggcctc tcctacgagg cgatgaacaa cgcggggcat
     2521 tccgaccgcg acctgatcgt cgtgctcaac gacaacggaa tgtcgatcgc ccccaacgtc
     2581 ggcgccatga gcaagtatct gggatcgatc atcgccagcc cggtcggcgt ccggtggcgg
     2641 gagcgcgtca agcacgccat cgagaaggct tcgcacctcg tgggcgggcc gcggctcgtg
     2701 gaattcgcca agaccgtgga ggagagcgtc aagaatctct ggtcaccggg catgctgttc
     2761 gaagagatgg gattccgcta cttcggtccc atcgcggggc acgatctgcc gcacctcgtg
     2821 tccactttcg agatcgtgcg ggggctcaag ggaccgcgcc tcgtgcacgt gctcaccgag
     2881 aagggcaagg gcttcccgct gcccgagccg gacgtggaga agtaccacgc gcgcgcgccg
     2941 tacgatccgg tgacgggcgt gctcaagccg gtggccccgg gcgcgccgca atggaccaag
     3001 atcttcggtg aagcgctcac ccagctcgcc ggcgagtacc cgcagctcgt cgccatcacg
     3061 gcggcgatgc cgtcgggcac aggcacgaat ctcttccaga agaaatggcc cgcgcggttc
     3121 ttcgacgtgg gcatcgccga ggcgcatgcg gtgaccttcg ccgccggcct cgcgacccag
     3181 ggcatccgtc ccgtcgtcgc catctactcg accttcctgc agcgcgccta cgacagcatc
     3241 gtgcacgacg tggcgatcca gtcgctgccg gtgatcttct gcctcgaccg cgccggcatg
     3301 gtgggcgagg acgggcagac gcacatgggg ctgtatgaca tcccctacct gctcgcgctt
     3361 cccaacatga cggtcacggc gcccaaggac ggtgccgagc tgatcggcct gttgcggtgc
     3421 gccctggcgc atcaggcggg tccgttcgcg ctgcgctatc cgcgcgacaa ggcgccgggg
     3481 gaggcaccgc tcgccgccga agtcacgccc gtgccctacg gcacctggga ggtgctgcgg
     3541 cgtggacgcg agtgcgccct gctcgcggtc ggcgtcatgt gccggccggc cctcgaggcg
     3601 gcggacctgc tggcgcgcga tggtttcgac gtcagcgtgg tgaactgccg cttcctgaag
     3661 ccgatggacc acgcgatgct cgacacgctg acacgcgatc acaagctgct catccccatc
     3721 gaggacgggg ccgtgacgaa cggcttcggc gcgacgctgg ccggtcagct gcagggcacc
     3781 gcctccgagg ttcgggtcgt gcccatcggc gcaccggacc ggacctacga gcacgcgccc
     3841 cgcgcgcgcc agctcgaagc agcgggtctc tccgcgaaag gaatcgccgc gcgggtccgc
     3901 gcattggcgg cggaagagag cctcgcccca cgatgaacgt cggggtcgtc ggcaacccca
     3961 gctaccacga cctccgcagc cttctcgcgc ggctgaccgc agcggctccg cggctcggct
     4021 tcacgctgta cagcgaggag cggctgctcg actactggcc tgaccagcgg ccgccggtct
     4081 ttcacgccga cacgccgctc gattgcctcc tcaccttcgg cggcgacggg accctgttgc
     4141 ggggcgcccg gttgctggac ggcgcctcga cgccgatcct cggcgtcaat gtgggtcgcg
     4201 tcgggtttct cgcgacggca ccggcgcaca atctcgattg ggtgctcgac gcggcggctc
     4261 gcaaggccta caccatcgag ccacggctcg cgctggtcgc gacggtcgag gaccgacagg
     4321 gcgcccgccg cccccagccc gtcgcgctga acgacatcgt cgtgcacaag ggcggggtgg
     4381 cgcgcgtgct gcgcttccgg gtgtccgtgg acggcgagga agtcgggacc tacagcgcgg
     4441 acggcatcat cgtcgccacg ccgaccgggt ccaccgccta ttccctctcc gccggcgggc
     4501 cgatcgtggt gcacggcgtg gatgctctcg tcgtcacggc catctgcccg cacacgatgg
     4561 cggtccggcc gctggtgctg ccgtcctcca cgacggtgat gatcgaacct gtcccaccct
     4621 ggtccgacac cgaggagatt ctgttgtcgc tcgacgggca gctcagccgg gcgctgcaac
     4681 ccggcgagcg gctgctcgtg cgccgcgccg agcgccccgt gctcctggga cggctcgggc
     4741 cggaagggtt ctttgcgcgc atgcgccgca aactcaactg gggagacctc gccgaccggg
     4801 agcgccgcga gtgctgaccg agctgcgcgt gcgcgacctc gcggtcatcg cggatgtcac
     4861 gctgcgcctc ggccctggcc tcaatgtgct cagcggtgag accggcgccg ggaaatccat
     4921 gctggtggac gcgctcgcgc tgctgctggg cgagcgcgcg tcggccgagg ccgtgcgtcc
     4981 cggtgcggcc cggacggtca tcgagggcgc cttcgagctc tccgccggca ccgcccgccg
     5041 ccttgcggcg ccgctcgccg cgctgggcgt cgaagtggag gaagggcggc tgattctcaa
     5101 gcgagaggtg agcggggaag gccgctcgcg tgcctgggtc aacggcagcc cggccaccgt
     5161 cggcgtcctc gcgcagttgg ggggattgct ggtggatctg cacggccaac acgacaccca
     5221 gtcgctgctg catgccgaga cgcagcgcga tctgctcgat gcctacgccg gagccgaggt
     5281 ggaacgggtc gccgtgcgcg atgcccacgc acgggtcaga gcactcgagg agcgggaggc
     5341 cgagctgcgc ggcaagcagg aggagatccg tcgcaaggcc gactacctgc ggcacgtcgt
     5401 ggaggagatt tcccgggccg ccccgaaggt cggagaggac gaggccctcg agggggaagc
     5461 ccggcggctg gcccacggcg acgagctggg ccggctgtcg cgcgacctcg aacaagcgct
     5521 cgatgccggc ggtctggccc gggcggcgaa gctgctggcg gcgctggagc ggctcgatcc
     5581 ctccaccggt tcctggcggg agctgctcga tacggcgttc gccaacgtga acgagctcgc
     5641 cgcggccgca cgcgaatacg gggcgggaat cgacgccgat ccggcccgcc tggaggagat
     5701 cgagcggcgc cgcgacacac tcttcagact gctgcagaag tacggaccgg ccatccccga
     5761 cgtgctcgcc gcgcgggacg ccgcggcgcg ggagctcgat ctgctcgaca acgccgacct
     5821 ggacctgcgc gatctctcgg cgcagcggga cgcggcggtg caggagttca cccgggcctg
     5881 tgcggccctg tccgagaagc ggcgccaggg cgcgcagcgg ctggcgcgcg ccctcaccgc
     5941 ccgactcacc ccgctcggca tgcccggggg ccggttcacg gtcgatctcg gatcctacac
     6001 ggctgcccgg gcggacgggg cggaaggcgt cgcgttcctc gtgcgtctga acgagggcat
     6061 ggaggcccgc ccgctggccc gcgtggcgtc cggaggcgag ctgtcgcggg tcatgctcgc
     6121 cctcaaggtc gtgctggccc accacgacgc ggtgcccacg ctggtgttcg acgaggtgga
     6181 tcaggggatc ggcggcgagg cggcggcgca ggtgggggcg gcgctcgcgg acgtcgtgcg
     6241 ggaaggggcc gagcacgagc gccaggccct ggtgatcacc cacctgccgc agatcgccgc
     6301 gcgtgccgac cggcagctcg ccgtggccaa gcgcgaccgc ggggggatcg cgaccagcga
     6361 cgtccgcctg gtcgagggag aggagcgcat ccgggagatc gcccggatgc tgggcgatca
     6421 cgactcggcg accgcccgcc gccacgccga agatctgctc agaacagcag aatcgtctgc
     6481 cgcatcacga tccgaaacac cgtcgcgacc ggcaccgggc cgcccgcgcc gctgatcgtc
     6541 cgctccactg agaagccccc ttccagtcgc ggcccggcaa acgtcgcggc tacgcccagt
     6601 cgcgcctgcc ggatcccggt tcccggttcg agcaccgagg cagagaccgg cccacccacc
     6661 gccgtcgcca cgtctgccga atcctggggc gcgagaaacg catagcgatc ccgctgctgc
     6721 gcgtagtagc cgaacgtgac gccgatcgcg aagaggcgcg agaatcggaa catcggggcg
     6781 acgtccaccg ccacgtagtc acctgggtcc catcgtaggc gcttccccgt cgaacgcgag
     6841 aggaacggct ggtccactgg gccgatgaga cgttcgcgct cccccggcaa ctgccgcgct
     6901 ccgcgtatcg acagattgag ccacagcctg tgcatcagcg taagctcggc cgtcagacga
     6961 ccttcgacgt cggtctggtg gtctccggtc gacaggtcga acggatcgaa gggcgaatcc
     7021 tggtgtcccg tggggaggcg aacgaggagg gcggcagcgg cagcgtagct gtctccgctg
     7081 aagaagcggt acttggcggc gacctcggcg tctccgggga agtaccgcag ccgtcggggg
     7141 gtgccgggac catacggcgt gaggcccagc tggctcagcg agtcagcgag aatcccggcc
     7201 gccgcttcgc ccggcacggg cacgacgggg agaaagaacg tatcgcggga gaacgtggcg
     7261 acgttgaatg tatcggcgag cgcgtgttgc agtcccagga cgataccggt gagactgcga
     7321 ccggcatccg acccctcggt cggcagaaac cttgcggtcg tatcgaacac ggcagtcgag
     7381 caggccgacg cgaacgctct cccttgggcg agcagcgcct gcgcgcgcag gtacgtggga
     7441 cttccggggc tcccatatgc tcccgcgttg atgttgacgc cgagctggtc gagggcggtg
     7501 cccaggtggc cgaagaagtc ggtgtaacgc gcccggtcag gcaataggcc gaggttggag
     7561 ccctcagggc tgaagcggta ccgctctcgc acctccaccc gcacgattgg gatcgtcgcc
     7621 ccgagtgaca gccgattcgt gatcccgtat tcgagctgga tgggcgtcac gcgtcgctcc
     7681 gagcggatgg cgagcaggct gcccccgagc cgcgcgacgt agcccgcgag tcccgtggcc
     7741 gcgcgcacgt tctcctgcgc gagggcgagc gccggctgcc atggcgtgag ggagtccccg
     7801 gcgaacgcgg cgcccaccgg ctggcggccc ccgggcgtga agacctcttc ccatgtcatg
     7861 atgcggggat cgaacgtgac gcgcagggcg ccgcgccgcg gcgtatcggt ccgctctacc
     7921 gcctgggcgg tgagccgccc caccgggagg caaacgatcg cggctgccgc gatcgaaagg
     7981 gagaagacag gtcgctggcg cattacagaa cggcacccgg caggcgccgg gtgccgtcct
     8041 cgttacccca gtacatgcgg ccggttcgcg tcagggaatc gcggcgatgc tggtgccata
     8101 cttggcgttg atcgcctgga tcagcgcatt cgccaccaat ttgtccgcta ggcctgtcgg
     8161 gtggattccg tcgcgactga agtacgtacc gaacggcgcc gtcaccgatg ccggtccggt
     8221 cgtcgtgggg aagggcggga tggaaccggg cggcagcgcg gtgaacaaag cgttggggtc
     8281 cacgtaggcg tacccgcggc cggttgcctg ggccgcaatg gtggcgttgt actggttcac
     8341 ggcccgcacc agtcgcacca actccgccgg ctgcacggtc tgcgtctccg tgcaactgag
     8401 cgtgaaggtg ccggccgggt tggcggcagc cagtccgatc aggccgaggc cgaactggaa
     8461 cggcaccagg accgagtcgc ccttgccgcc cagcgcgctg ggcgcgcagt tcgggccgac
     8521 gatgaagttc gggggaaagt tggctgtcgt gttcttgatc gcgaaatacg tctggccctg
     8581 cgaaaagaac ggaatcagag tcacgttcgc cacgccgatc aacacgccct tgccgcttgc
     8641 gggtgtcgcg tcgatcgcat cgaggacggc cttgtagcga ttcgcgaaca gcgtgctgtc
     8701 ggtgactttg gtcgagtctc cggcattggc ggcgctgatc gcggcgccga gcacgtcgtt
     8761 gttgcccagc caaacggtga cgaacgtcgg gttggcgcgc tgcatcgcct ggctctgcgt
     8821 cagcccgccg aggatgagcg tcgtgagcgg gttggcgttg gaggcggtgt cgaggttcga
     8881 ggtgccgtcg atcacctcgg cggctggcac ggcgacgtcg ctgatgtacg gcggcgggat
     8941 cggctggaac tggcgcagcg cgcaggtgga cgccgtgccg ccaccgacgc gatgcggcgt
     9001 accggaagct cgaaacagcg tatcgatcgg cggcgggcag ccgggtcggt tcaagagcgg
     9061 catgaagaac ggtgtgtgca tctgcttgct cagcagattg gggtacgcca gcagctgcgt
     9121 gctgtcgttg attccccccg actggaatcc ggctgtgagc gaattgccca tcgccacgta
     9181 gcgcgtgaac agtggatcga tcggctgcgt gttggccggc cggaacagct cgtcgttgtg
     9241 acacgcgacc gcgaggagaa cgccggtgag ggctgcgccg aggaacttgg tggttcgtgt
     9301 catggtgttc gcgtctcgcg ctcagaaagt aaagtgcagc ccggcgccga acaggtgggc
     9361 cgagaattcg tacagccccg tgttgctcac cggcagcggg aacacccgcc cgcgccggtc
     9421 ctgctgggcg atgtactggt aggccaagtc cccgtgcagc ctgggggtga agtcgtatcc
     9481 cacgccggcc gtgaattcgt tgcgcggcgc ctcgggaagg agcggagtca cgaaatcgac
     9541 cggcgaggcg gcggtgtggt agaggtagcc gccgcgcagc gtgagcttcg ccgagggctc
     9601 gtactccgtt ccgatccgga atccgtaggt gtccttgttc ttcggactga gactgagggt
     9661 gggcgtcgcc gggttcgcga atttgatcgc gacgctctgg aaccaccccc agacgacgta
     9721 ctgaaaatcc gccatgaccg accaattgtc ccgcatctgg tacgcaaatc cgaacgtgcc
     9781 ctggggcggc atggtaatcg aggtgctggc gccgccgttg gcgagcgggc cgccggcggc
     9841 gaactcggct gcgagtaatg catccaccgg cgtgccgccg ggcagcccca gcgggttgtt
     9901 gggcggcagc gtgaggcccg tgggaatctg atcgaacaca gcgtcgccgt cgtacgagat
     9961 ggtcttcctc gtgatccagt ggccgccgat ggacagccgg tcggtgagct tgatgatgcc
    10021 cccgaaattg accgcgaacc cgttgccgct cgcgctcagg tccgcgttcg cgaaatcggt
    10081 gcccggcagc acgcccaggg ccccgaaggt gatccccggt gcgggggact gcgtcgccag
    10141 gtcgaggcgc tcgtgcagct ccagcgtgct gcggatgtag gccaccccca cccccacctt
    10201 gagccagccc ccgacctgat agccgagggt cggctggacg tagatggagt ggatcttggt
    10261 attgtagccc atgaagcggc cgtcgaatcc cgcgatcggc cacttcgttt ccaggccgta
    10321 gggcgcgtac acgccgacgc cgaccgtcag cttggggctc accgcgtagg tgaggtaccc
    10381 gttcggcacc gggatggtgg gattcgcaag gtcggtcttc ttagcgaaga gatcgtcggt
    10441 gaagccgccc tgggcgtgga tcaacgtgac gccggcgctc acatgcccgc cggagagacc
    10501 gccgagcccg gcggggttga agaagatggc cgagccgtcg gcgcagggac gggcggccga
    10561 tacccctccg cgacccatgg tacacgtgtt gtgctcgtac acaccgaacc cttgggcgcc
    10621 gagcagggaa ggaacgccgg tgagcagcaa ccacgtaaac cgacgcatgt gcggatctcc
    10681 gaggaggccg gatggtggag atagtggaaa aggctggcat acggtaccat tcgcctcaac
    10741 ccttgtcaaa cgtatgcgaa ctcaagcgat agctctgttc caccgcgtct tcggcggcgc
    10801 gcccggcacc gtcgcgagcg cgccgggtcg cgtgaacctg ctgggtgagc ataccgacta
    10861 caacggcgga ccggtcctgc cgatgg
//
LOCUS       DBNGZW010000672         4447 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_948728, whole genome shotgun sequence.
ACCESSION   DBNGZW010000672 DBNGZW010000000
VERSION     DBNGZW010000672.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4447)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4447)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4447
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_948728"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..693
                     /locus_tag="AC16JC_15860"
     CDS             <1..693
                     /locus_tag="AC16JC_15860"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR5166225.1"
                     /translation="TGIALAAIACQAPGERRNVEWIDATATLDPATTPVYEGDAPMRF
                     DFLKDMRRGDPLTLSVFSLGAHSGTHVDAPMHFVADGAPVDRIALEALVGPARVVQIP
                     DSVQAIDAVELARHQWRGAERVLFRTRSTERRWMSSAEFHRDFAYIAPDAAQLLRDAG
                     VKLVGIDYISAEQFGAPAPRTHQLLLGGGIPIVEGLDLSDVPAGDYDLIVLPMKVRGH
                     EGAPARAIVRKR"
     gene            complement(700..1611)
                     /locus_tag="AC16JC_15865"
     CDS             complement(700..1611)
                     /locus_tag="AC16JC_15865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009541084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR5166226.1"
                     /translation="MPVRIGIDLGGTKIEGIALSSAGEEIARRRAATPPDYMASLDAI
                     ATLVRELQPDTDQATVGVGIPGAIVPRTGLVKNANSTWLNGRPLGRDLEHRLGRPVRL
                     MNDANCFAMSEATDGAAAGAHVVFGVILGTGVGGGIVVDGRCLLGANFIAGEWGHNPL
                     PWPAAEEQPGPSCYCGKRGCVESWLSGPGFERDHAAHTGRTLSSREIVRAAERGDAGA
                     AATLARYHDRLGRALVSLINVLDPDVVVLGGGMSNIAGLPEAASAVLPRYLFAAGARA
                     EPVATRVVRAAHGDASGVRGAAWLWPR"
     gene            1688..4435
                     /locus_tag="AC16JC_15870"
     CDS             1688..4435
                     /locus_tag="AC16JC_15870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015330832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HIR5166227.1"
                     /translation="MRPSFLLSLAALLALPGPPERPLGVTGLRVEYLTDPLGIDVARP
                     RLSWRITCTERNTVQSAYQLQVARSDADLARGEHLLWDSGRITSDASVFVDYAGPALE
                     SGTRYVWRVRVWDRGGHASGWSAPGAWETGLLQPADWTAAWIGTAPSPADTLPSPSPL
                     LRRPFRVSGAVRTARLYATSRGLYEVYLNGRRVGDRLFTPGWTSYHRRLQYQTYDVSS
                     FLRPGDNAVGAMLGDGWYRGQLGFFGQRNLYGRRLALRLQLEIRYRDGRTERVVSDSQ
                     WKTTSGPVLGSDIYGGEAYDARRERPGWSAAPYDDRDWAPVATVEPPAAALVASESPP
                     VRRVRELRPVVIHRAPAGETVFDLGQNFTGWARLAVRGPAGTVVTLRYAEVLDRDGNL
                     YTANLRRAAQTDRYTLKGEGEERYEPHFTFHGFRYVGVTGLPAAADSGTITGIAVSSD
                     LPTTGTFETSDSLLDRLQQNIVWGQRSNFLDVPTDCPQRDERLGWTGDAQVFARTAAF
                     NMDVAGFFSKWLADLAADQDASGSLPWVIPNPLGGDSTRFAGTAGWGDATVIVPWTVY
                     LAYGDRRLLERQYPSMRAWVEYERRRAGAELIWRPGWQFGDWLAFHSDDPSYPGATTG
                     TDLIATAFLAHSADLLARAAAALGRDDEAARYGVLFRDVRMAFDREFVTGNGRVGENT
                     QTAYALALAFDLLPDSLVPAAAERLAADIRARDVHLTTGFLGTPHLLPVLSATGHLDL
                     AYELLTQRSYPSWLYPITRGATTMWERWDGIRPDSTFEDPGMNSFNHYAFGAVGDWMY
                     RTIGGIDADPAAPGYRKARIAPHPGAGLTHARASLETLYCTLASAWRRDGGQFVLEVT
                     VPPNTSAEVTLWETEVGRIREGGRPVSDAPGVHAVRRDGSDVIVTIGSGSYVFTVARA
                     P"
BASE COUNT          624 a         1605 c         1553 g          665 t
ORIGIN      
        1 acgggaatcg cactggccgc gattgcgtgt caggcgcctg gcgagcggcg gaatgtggag
       61 tggatcgacg cgacggcgac gctcgatccc gcgacgacgc cggtctacga gggcgacgcg
      121 ccgatgaggt tcgacttcct caaggacatg cgacgcggcg atccgctcac gctgtcggtc
      181 ttctcgctgg gagcgcacag cggaacgcac gtcgacgccc cgatgcactt cgtggccgac
      241 ggcgcccccg tggaccggat cgcactggag gccctcgtag gccctgcacg cgtggtgcag
      301 attcccgaca gcgtgcaggc gatcgatgcc gtcgagctcg cccggcatca atggagaggc
      361 gccgagcggg tgctgtttcg caccaggagt accgagcgcc gctggatgag ctccgccgag
      421 tttcaccgcg acttcgccta catcgccccg gatgccgcgc agctcctgcg cgacgcgggg
      481 gtcaagctcg tcggcatcga ttacatctcg gccgagcagt tcggcgctcc ggcgccccgc
      541 acgcaccagc tcctcctcgg cgggggcatc ccgatcgtcg aaggattgga cctgagcgac
      601 gttccggcgg gcgactacga tctgatcgtc ctgcccatga aggtgcgcgg acacgagggg
      661 gcgccggcac gggcgattgt gcggaagcga taggggccgt catcgcggcc aaagccacgc
      721 cgcccctctg acgccgctcg cgtcaccgtg cgccgcgcgc accacccgcg tggcgacggg
      781 ctcggcgcgc gccccggccg cgaagagata gcgagggagc accgccgacg cggcctcggg
      841 cagccccgcg atgttcgaca tgccgccacc caggaccacg acatccgggt cgagcacgtt
      901 gatgagcgag accagcgcac gacccagccg gtcgtggtac cgggccaggg tcgccgcggc
      961 gccggcatca ccgcgctccg cggcgcgcac gatctcccgg ctggacaagg tgcgcccggt
     1021 atgcgcggcg tgatcgcgct cgaaacccgg cccggaaagc caggattcga cacagccgcg
     1081 cttgccgcag tagcaggatg gcccgggctg ctcctcggca gcgggccagg gcagcggatt
     1141 gtgtccccac tcgccggcga tgaagttggc gcccaacagg caccggccgt cgacgacgat
     1201 cccgccgccg actcccgtgc cgaggatgac gccgaagacc acatgggcgc cggccgccgc
     1261 gccgtccgtg gcttcggaca tggcgaagca gttcgcgtcg ttcatgagcc gcaccgggcg
     1321 gccgagtcga tgctcgagat cgcggcccag cggccggccg ttgagccagg tcgagttggc
     1381 gttcttcacg agccccgtgc ggggcacgat cgcccccggg atgcccacac cgacggtggc
     1441 ctggtcggtg tcgggttgga gctcccgtac cagggtcgcg atggcgtcga gcgacgccat
     1501 gtagtccggc ggcgtggcgg cgcgacgtcg ggcgatctcc tccccggcgg acgacagggc
     1561 gattccttcg atcttcgttc cgccgaggtc gatgccgata cgcacgggca tgacggaacg
     1621 tatctccgac tcgccacccg cgccctgctg tctggtgtgg gccttcgcca gtcatagatt
     1681 ggggagcgtg aggcccagct tcctgctctc gcttgccgcg ctcctggcgc tccccggtcc
     1741 gcccgagcgc ccactcggcg tcaccgggct ccgggtggag tatctcaccg atcccctggg
     1801 cattgacgtc gcgcgacccc gcctgagctg gcgcatcacg tgcaccgagc gcaacacggt
     1861 gcagagcgcc taccagctgc aggtcgcccg gagcgacgcc gacctcgccc ggggcgagca
     1921 cctgctgtgg gattccggac gcatcacctc agacgcgtcg gtcttcgtcg attacgccgg
     1981 tccggcgctc gagtcgggga cccgctatgt ctggcgggtg cgcgtgtggg accggggcgg
     2041 ccacgcttcc ggctggagcg cacccggcgc gtgggagacc ggcctgctcc agcccgcgga
     2101 ttggacggcc gcatggatcg ggacggcccc gagcccggcc gacaccctcc cctccccctc
     2161 gccgctcctg cgccgtccct ttcgtgtgag cggcgcggtg cgcaccgccc ggctctacgc
     2221 gacgagtcgc ggcctgtacg aggtctatct caacgggcgc cgcgtgggtg accggctgtt
     2281 cacacccggc tggacctcct accaccgccg cctgcagtac cagacctacg acgtcagctc
     2341 gttcctgcgg cccggcgaca acgccgtcgg tgccatgctg ggggacggct ggtaccgcgg
     2401 gcagctggga ttcttcggcc agcgcaacct ctacggccgg cgcctcgcgt tgcgcctgca
     2461 gctcgagatc cgctaccggg acggccgcac ggagcgcgtc gtctccgact cgcagtggaa
     2521 gacgacctcc ggcccggtcc tcggctccga catctacggc ggcgaagcgt acgacgcgcg
     2581 gcgggagcgt cccggctggt cggctgcgcc ctacgacgac cgtgactggg cgcccgtcgc
     2641 cacggtggag ccgccggccg ccgcgctcgt cgcgtccgag tcgccgccgg tgcgtcgcgt
     2701 acgcgagctg cgccccgtgg tcatccaccg ggctcctgcg ggtgagaccg tcttcgatct
     2761 cggccagaac ttcaccggat gggcccgcct ggcggtccgc ggtcccgccg gcacggtcgt
     2821 cacgctgcgc tacgcggagg tgctcgatcg cgacgggaat ctctacaccg ccaatctccg
     2881 gcgggcggcg cagaccgatc gctataccct caagggcgaa ggggaggagc gctacgagcc
     2941 gcacttcacc ttccatggat tccgctacgt gggcgtgacg gggcttccgg ctgcggcgga
     3001 tagcggcacc atcacgggga tcgccgtgtc gtccgacctc ccgacgacgg gcaccttcga
     3061 gacttccgat tcgctcctcg accggctgca gcagaacatc gtctgggggc agcggagcaa
     3121 cttcctcgat gtgccgaccg actgtccgca gcgcgacgag cggctgggct ggacgggcga
     3181 cgcgcaggtg ttcgcgcgga ccgccgcgtt caacatggat gtcgcgggat tcttctcgaa
     3241 atggctcgcg gatctggcgg cagaccagga tgcgagcggc agcctcccct gggtgattcc
     3301 caacccgctg ggcggcgact cgacgcggtt cgccggcacc gcaggttggg gcgacgccac
     3361 cgtaatcgtg ccgtggaccg tgtatctcgc ctacggcgac cggcgcctgc tggagcggca
     3421 gtacccgagc atgcgcgcct gggtcgaata cgagcgccgc cgggccggcg ccgagttgat
     3481 ctggcgaccg ggctggcagt tcggcgattg gctcgcgttt cacagcgacg atccgtcgta
     3541 tcccggcgcc accaccggga ccgacctgat cgccaccgcc ttcctcgccc actcggccga
     3601 tctcctggcg cgcgccgcgg ctgccctggg acgtgacgac gaagcggccc gctatggggt
     3661 gctgtttcgc gacgtgcgca tggccttcga tcgggagttc gtcaccggca acggacgggt
     3721 gggcgagaac acgcagacgg cgtacgcgct ggcgctcgcc ttcgacctcc tgcccgattc
     3781 gctggtgccc gcggccgccg aacgcctcgc cgcggacatc cgcgcacggg acgtgcacct
     3841 caccaccggg ttcctgggca ccccgcacct cctccccgtg ctgagcgcca ccggccacct
     3901 cgacctcgcg tacgagctgc tcacccagcg gtcctatccc tcgtggctct atccgatcac
     3961 ccgcggggcg acgacgatgt gggagcggtg ggacggcatc cggccggaca gcacgttcga
     4021 ggatccgggg atgaattcgt tcaatcatta cgcgttcggc gcggtgggcg actggatgta
     4081 ccgcaccatc ggcgggatcg acgccgatcc ggcggcgccc ggataccgga aggcgcgcat
     4141 cgcaccgcac ccgggcgcgg gcctcacgca cgcgcgagcg tcgctcgaga ccctctactg
     4201 cacactcgcc tccgcgtggc ggcgggacgg cgggcagttc gtgctcgagg tgaccgtccc
     4261 gccgaacacg tccgccgagg tcacgctgtg ggagacggag gtcggccgca tccgggaggg
     4321 ggggcgtcca gtgagcgacg caccgggcgt gcacgctgtt cggcgggacg gcagtgacgt
     4381 cattgtgacc attggatcgg gcagctacgt gttcacggtt gcccgcgcgc cctgagtctt
     4441 cgcgccg
//
LOCUS       DBNGZW010000673         2840 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_62617, whole genome shotgun sequence.
ACCESSION   DBNGZW010000673 DBNGZW010000000
VERSION     DBNGZW010000673.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2840)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2840)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2840
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_62617"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..595)
                     /gene="rimI"
                     /locus_tag="AC16JC_15875"
     CDS             complement(65..595)
                     /gene="rimI"
                     /locus_tag="AC16JC_15875"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 - protein-N-terminal-alanine
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HIR5166228.1"
                     /translation="MRPRPSPTTDVLRRHRSAGRRVTDARCRIRPAQAADLPALVAIE
                     RIAFTDPWSRGDFEDCLAMGVQIFVADGDNGVLGYIVGRAVRDQSEILNLGVTLNARR
                     RGIGASLVRQLLASFAAAGVREVFLEVRESNLAAQGLYRGFGFQDVGRRRRYYRRPVE
                     DAVILRAAIPAVEPSA"
     gene            complement(489..1319)
                     /gene="tsaB"
                     /locus_tag="AC16JC_15880"
     CDS             complement(489..1319)
                     /gene="tsaB"
                     /locus_tag="AC16JC_15880"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HIR5166229.1"
                     /translation="MAGEGGTHDAAARPVLHPRPRHRSGLPHAGGALSESWLAIDTAT
                     GVASVAAGTPPSADSVTVIAGARSQAAQLIELADRTLGAVGLRAADLAGLIVADGPGS
                     FTGLRIGWAAAKGLAQERGLPIVAIPALLAAATGAAAVLGPVPVAASFDALRGQVFAA
                     VYVVHAARVETVVAPLTCTPEELVARCPLRPRVAVGDGAMRYADAMERWTGTAPLSHL
                     SLQPAAVSLLALHPRADAGRILADAAAAEPDYGRPAEAQVRWEARHGRPLSHPSGSGS
                     "
     gene            complement(1220..1624)
                     /gene="tsaE"
                     /locus_tag="AC16JC_15885"
     CDS             complement(1220..1624)
                     /gene="tsaE"
                     /locus_tag="AC16JC_15885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0002949 - tRNA threonylcarbamoyladenosine
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HIR5166230.1"
                     /translation="MRLHVDELNQFGERLGRELRAPAIIGFSGDLGSGKTTLIQAMCR
                     GLGVAVPATSPTYALVHRYESPAGTVFHVDCYRLSSSREARDLGLEDILTQRAILLVE
                     WPEKAGHMMPPLDRYFILAHDTDPDYRTLEER"
     gene            complement(1587..>2840)
                     /locus_tag="AC16JC_15890"
     CDS             complement(1587..>2840)
                     /locus_tag="AC16JC_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007290150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HIR5166231.1"
                     /translation="IRAELAERLVLLRNSGKLLEAQRLESRTNFDIEMMLEIGTCAGI
                     ENYSRHLSGRAAGERPACLLDYFPEDFLIVVDESHVSLPQIRGMYNGDRARKEVLVEY
                     GFRLPSALDNRPLIFDEFLNLTPRALFVTATPGDLELQLSEGVIVEQIIRPTGLVDPE
                     IEVRPVRGQVDDLLHEIRLRERRGERVLVTTLTKRMAEDLTDYLQQTGVRVRYMHSDI
                     DAIERMEILRDLRLGKFDTLVGINLLREGLDLPEVSLVAILDADQEGFLRSDRSLIQT
                     VGRAARNLHGKAILYADRITGSRQRALDEMTRRRTRQQAYNVEHDITPRSIVKSVGDI
                     RFVTRVADARGSRVPESPVRRPVDAAEREAMIRVLESQMQAAAEALDFELAALLRDQL
                     LELKAELEPRGGRRAPARGRAQPVR"
BASE COUNT          470 a          990 c          960 g          420 t
ORIGIN      
        1 atgccccaga ttatatgacc cgcaagtcat tctgtcaatc aaagatacgc cacttgtacc
       61 atttctacgc agacggctcg actgccggaa tggcggcccg gaggatcacc gcgtcctcca
      121 ccggccggcg gtagtaccgt cggcgacgac cgacgtcctg aaagccgaac ccccgataca
      181 gcccctgggc cgcgaggttc gattcgcgca cctccagaaa tacctcgcgc acgccggcgg
      241 cagcgaacga tgcgagcagc tggcgaacca ggctggcccc gatgccccgg cggcgggcgt
      301 tcaacgtgac gccgaggttc aggatctcac tctggtcgcg caccgcacgg ccgacgatat
      361 agcccagtac cccgttgtcg ccgtcggcga cgaagatctg cacgcccatg gcgaggcaat
      421 cctcgaaatc gcctcgggac cagggatccg tgaaggcgat ccgctcgatg gcgaccagtg
      481 ccggaagatc agctgcctga gccggacgga tgcgacagcg ggcgtccgtg acgcgcctcc
      541 cagcggacct gtgcctccgc aggacgtccg tagtcgggct cggccgcggc cgcatcggcg
      601 aggatgcggc ccgcatcggc ccgcgggtga agcgcgagga gcgatacggc cgcgggttgg
      661 agactcaggt gagagagcgg cgccgtcccg gtccaccgtt ccatggcgtc ggcatagcgc
      721 atcgcgccat cgcctaccgc cacgcggggg cgcagcggac agcgggcgac gagctcttcc
      781 ggcgtgcagg tcaggggggc gaccacggtc tcgacgcgtg cggcgtgcac cacgtacacg
      841 gcggcgaaca cctgcccgcg caacgcgtcg aaggaggccg cgaccggaac agggccgagg
      901 acggccgcgg cccccgtcgc cgccgccagc agcgccggta tggccacgat ggggaggcca
      961 cgctcctgtg cgagcccctt ggcggccgcc cagccgatcc gcaaaccggt gaaactgccc
     1021 ggcccatcgg cgacgatgag gccggcgagg tctgcggccc gcagaccgac tgctccgagg
     1081 gtgcggtccg ccagctcgat gagctgcgcg gcctgcgacc tcgcgccggc gatcaccgtc
     1141 acgctgtcgg ccgagggggg cgtgcccgcg gcgaccgacg ccaccccggt ggcggtgtcg
     1201 atggcgagcc aggactcgct cagcgctcct ccagcgtgcg gtagtccgga tcggtgtcgt
     1261 gggcgaggat gaagtaccgg tcgagcggcg gcatcatgtg tcccgccttc tccggccact
     1321 cgaccagcag gatcgcccgc tgcgtgagga tgtcctcgag acccaggtcg cgggcctccc
     1381 gggaggagct gaggcgatag cagtcgacgt gaaacacggt gcccgccgga gactcgtagc
     1441 ggtgcaccag cgcgtatgtc gggctcgtgg ccggcaccgc gacgcccagc ccgcggcaca
     1501 tggcctggat cagcgtcgtc ttcccggatc ccagatcgcc ggaaaagccg atgatcgcgg
     1561 gagcgcgcag ctcgcgcccc agacgctcac cgaactggtt gagctcgtcc acgtgcaggc
     1621 gcacgtcgtc ctccccgtgg ctcgagctcc gccttcagct cgagcagttg atcccgcagc
     1681 agcgcggcca gctcgaagtc gagcgcctcg gcggcggcct gcatctggga ttcgagcacc
     1741 cggatcatcg cctcgcgctc ggcggcgtcg acggggcgcc gcaccggcga ctcgggcacg
     1801 cgactgccgc gcgcatccgc cacacgggtc acgaaacgga tgtcgccgac cgacttgacg
     1861 atcgagcgcg gcgtaatgtc gtgctccaca ttgtacgcct gctgcctggt gcgccgccgc
     1921 gtcatctcgt ccagggctcg ctgcctcgag ccggtgatcc ggtccgcata caggatcgcc
     1981 ttgccgtgga ggttgcgggc ggcgcgtccc acggtctgaa tgagcgagcg atccgagcgc
     2041 aggaagccct cctggtccgc atccaggatg gccacgaggg agacttccgg caaatccagt
     2101 ccttcgcgca gcaggttgat gccaaccagc gtgtcgaatt tgcccagacg gagatcacgc
     2161 agaatttcca tgcgctcgat ggcgtcgatg tccgagtgca tgtaacgcac gcggacgccc
     2221 gtctgctgga gatagtcggt gaggtcctcc gccatccgct tggtgagcgt cgtgacgagc
     2281 acgcgctcgc cccgccgctc gcgcagccgg atctcgtgca ggagatcgtc cacctggccg
     2341 cgcacgggcc gcacctcgat ctcggggtcg acgaggcccg tcggacggat gatctgctcg
     2401 acgatgactc cttccgacag ttgcaactcc aggtcccccg gagtcgcggt gacgaagagc
     2461 gcccgcggcg tcaggttgag aaactcgtcg aagatcaggg gccggttgtc gagggcgctc
     2521 gggagccgga agccgtactc caccagtacc tcctttcgcg cccggtcgcc gttgtacatg
     2581 ccccggatct gcggcagcga cacgtgcgac tcgtcgacga cgatcaggaa gtcctcaggg
     2641 aagtaatcga gcaggcacgc cgggcgctcg cccgcggcgc gaccggagag gtgacgcgag
     2701 tagttctcga tacccgcgca cgtcccgatc tcgagcatca tctcgatatc gaagttcgtt
     2761 cgcgactcga gtcgctgcgc ttcgagcagc ttcccggaat tccgcaacag gacgaggcgc
     2821 tccgccaatt ccgcccgtat
//
LOCUS       DBNGZW010000674         4333 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_815162, whole genome shotgun sequence.
ACCESSION   DBNGZW010000674 DBNGZW010000000
VERSION     DBNGZW010000674.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4333)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4333)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4333
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_815162"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="AC16JC_15895"
     CDS             complement(<1..239)
                     /locus_tag="AC16JC_15895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166232.1"
                     /translation="MIGARFDRVLVLTSALLLLAGAGLEADEPVAGLVAPYLRARSDR
                     ALGTVAVQAYSEPTRPNANPTPQPSVSVTLIPYSAV"
     gene            complement(236..1147)
                     /locus_tag="AC16JC_15900"
     CDS             complement(236..1147)
                     /locus_tag="AC16JC_15900"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR5166233.1"
                     /translation="MSGSPPRAGRVAATVGLWGLLLVGWVPAHLPPALGLAAGRRIGD
                     VLWWLLPRRRRVALDNIRRSLGREMAPEQIRRLGRRSFQHVGMNLVEACRYVLRPTDV
                     MLSRVRVDGVEHLRGAAAHGRGVLILTAHYGNWELLAAAHGLTGLPLSIVIRPLDHPV
                     LDELAARFRRRSGAELIVKHLAVREVLRDLRRGRMVGILLDQNATRSEGVFVPFFGIP
                     ASTSKGLAVLALRTGAPVVPVFLRREPDGRHCMDVGPPLVPPPDGDIEAYTATFNLVI
                     EAAIRRAPEQWLWMHARWRTRPREVSS"
     gene            complement(1144..2151)
                     /locus_tag="AC16JC_15905"
     CDS             complement(1144..2151)
                     /locus_tag="AC16JC_15905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015434433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005315 - phosphate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HIR5166234.1"
                     /translation="MSGSLLLVVLIILVALAFDYINGFHDAANSIATVVSTRVLTPMQ
                     AVAWAAFFNFVAAFGFGVQVARTVGKGVVDAGVVDQWVILGGLCGAITWNLITWYYGI
                     PTSSSHALIGGYAGAAVAKAGFAALLASGLLKILVFIVLAPVIGLGLGFGLMVITLWT
                     VRSIRPSRVDGLFRRLQLISAAAYSLGHGTNDAQKTMGIIAILLFSAGYLGSEFYVPM
                     WVILAAHAAIGLGTLAGGWRIVKTMGMRLTKLRPIGGFCAETAGAITLLGTAVAGIPV
                     STTHTITGSILGVGATQRLSAVRWGVASNIVWAWILTIPLSGLIAAMVWGVTNLVLHV
                     G"
     gene            complement(2171..2788)
                     /locus_tag="AC16JC_15910"
     CDS             complement(2171..2788)
                     /locus_tag="AC16JC_15910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011523930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HIR5166235.1"
                     /translation="MFRLLPREEKFFDLFEQQATHIVSASRVLEELTLDYADAKAKAQ
                     QIKDLEHAGDTLTHEIVRRLNTTFITPIDREDIYALASRLDDVLDLIDAVADRLLLYK
                     IKAPTDGCIGMAKIIVKTAEETDRAVRCLRTLSPFYHKHAVEVNRLENEADRLLRDQL
                     AALFEGGTDAIEVIKWKELYETMESVTDRCEDVVNVIEGITLKMA"
     gene            2873..3088
                     /locus_tag="AC16JC_15915"
     CDS             2873..3088
                     /locus_tag="AC16JC_15915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004975985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HIR5166236.1"
                     /translation="MARSEPCARRQVRCPRCGASCGWTGNPHRPFCSLECRLIDLGAW
                     LDERYRVPGPELPSDSESPERGAPSGG"
     gene            3085..3741
                     /locus_tag="AC16JC_15920"
     CDS             3085..3741
                     /locus_tag="AC16JC_15920"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006003040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR5166237.1"
                     /translation="MIDLFDDPLGFLSRLILQIPALLVAVTVHELAHAVVADRLGDPT
                     ARRLGRLTLNPLPHIDPVGAIAFVLAGFGWAKPVPVNAQNLRHPVRDMAWVAAAGPLS
                     NFTAAFVALVAVRVLGRLDGVAPSLVSEPLAGVLFWTYIFNLALGIFNLIPLPPLDGG
                     HFLPYLIPRASWSFIHQLEQVGPFLLILLLLSGATRYVVQPVFNAVSAFYLSIVRLVI
                     "
     gene            complement(3692..>4333)
                     /locus_tag="AC16JC_15925"
     CDS             complement(3692..>4333)
                     /locus_tag="AC16JC_15925"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011420027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR5166238.1"
                     /translation="PGRKARALSRSGLSGVMSRMRTPLDDRVLFDEIVSPIGRIVIAA
                     RDGRLCALEFGRARAVRSVAARYGPTRPTWTRDPFGISRKIRAYLAGDLAAVDRIPVD
                     TGGTPFQRKVWQALRRIRPGRTRSYGELARALGHGAAARAVGAANGRNPISIVVPCHR
                     LIGGDASLTGYGGGLWRKRWLLRHEGADPAKARRGARARSRAGRSTGRRRSPH"
BASE COUNT          665 a         1518 c         1502 g          648 t
ORIGIN      
        1 accgccgagt acgggatcag cgtgacggat accgagggct ggggcgtcgg gttggcattg
       61 ggacgggtcg gctcgctata ggcctgcacc gccacggtgc ccagagcccg gtcgcttcgc
      121 gccctgagat acggggcgac gaggccggcg accggctcgt cggcctcgag gccagcgccg
      181 gcgaggagca gcagggcgct cgtgaggacc aggacccggt cgaagcgggc tccgatcacg
      241 agctcacctc ccgcggacgg gtccgccacc gggcgtgcat ccacagccac tgctcgggcg
      301 cacgacggat cgccgcctcg atcaccaggt tgaacgtcgc ggtgtaggcc tcgatgtcgc
      361 cgtccggcgg agggacgagg ggcgggccga cgtccatgca gtggcgtcca tccggctccc
      421 tccggaggaa gaccggcacc accggcgcgc ccgtccggag ggcgaggacc gccagcccct
      481 tcgaggtcga ggcgggtatc ccgaagaacg gaacgaacac cccttcggag cgcgtggcgt
      541 tctggtcgag gaggatgccc accatgcggc ctcgacggag gtcccgcagc acttctctca
      601 ccgccaaatg cttgacgatg agctcggcgc cgctgcggcg ccggaatcga gcggccagct
      661 cgtcgaggac gggatggtcc aggggacgaa tcacgatgga cagggggagg ccggtcagcc
      721 cgtgggcggc cgccagcagc tcccagttgc cgtagtgggc ggtcaggatc aggacccccc
      781 ggccgtgggc ggccgcgccc cgcaggtgct cgaccccatc gacgcggacc cgcgagagca
      841 tgacatcggt gggacggagg acgtagcggc aggcttcgac gagattcatc cccacgtgct
      901 gaaaggatcg gcggcccaga cgccggatct gctccggggc catctcccgg cccaggctac
      961 gccggatgtt gtccagcgcc acgcgccgac gccggggcag gagccaccac agcacgtcgc
     1021 cgatgcgccg gccggccgcg agccccaggg ccggtggaag gtgggccggc acccatccca
     1081 cgaggaggag gccccagaga cccacggtcg ccgccacccg gccggcgcgt gggggggacc
     1141 cgctcatccg acgtggagga ccagattggt gacgccccac accatggcgg cgatcagccc
     1201 ggacagcggg atggtcagga tccaggccca gacgatgttg ctggcgacac cccaccggac
     1261 ggcggacaga cgctgggtgg cgcccacccc caggatcgag ccggtgatgg tgtgcgtggt
     1321 actcaccggt atcccggcca ccgccgtgcc gagcagcgtg atcgctccgg cggtctcggc
     1381 gcagaagcca ccgatcgggc gcagcttggt caaccgcatt cccatcgtct tcacgatgcg
     1441 ccagcccccc gcgagtgtgc cgagcccgat ggcggcgtgc gcggccagga tcacccacat
     1501 cggaacgtag aactccgatc ccaggtatcc cgccgagaag agaaggatcg cgatgatccc
     1561 catcgtcttc tgcgcgtcat tggttccgtg accgaggctg taggccgcgg cggagatcag
     1621 ctggaggcgg cggaagagcc cgtccacccg gctgggccgg atcgagcgca cggtccacag
     1681 cgtgatgacc atcaaaccga agcccagacc caggccgatc acgggagcca gcacgatgaa
     1741 gaccaggatc ttgagcagcc cgctcgccag gagcgccgcg aagccggcct tcgccaccgc
     1801 cgcgcctgcg taccccccga tcagcgcgtg cgaggagctc gtggggatgc cgtagtacca
     1861 ggtgatgagg ttccaggtga tcgcgccgca cagcccaccc aggatcaccc actggtccac
     1921 cacgcccgcg tcgaccacac ccttgcccac cgttcgcgcc acctggacgc cgaagccgaa
     1981 ggcggcgacg aagttgaaga aggcggccca cgccaccgcc tgcatcgggg tgagcacccg
     2041 ggtggagacc acggtggcga tcgagttggc cgcgtcgtga aacccgttga tgtaatcgaa
     2101 ggcgagggcc accaggatga tcagcaccac cagcagcagg gacccgctca tgcgcggcga
     2161 gcgggccggt tcaggccatc ttgagggtga tgccctcgat cacgttgacc acgtcctcgc
     2221 accgatccgt gaccgactcc atagtctcgt acagctcttt ccacttgatg acctcgatgg
     2281 cgtcggtgcc accctcgaag agggccgcga gctggtcgcg caggaggcgg tctgcctcgt
     2341 tctcgagacg attcacctcg accgcgtgct tgtggtagaa gggcgacagg gtccgaaggc
     2401 agcgcaccgc ccggtccgtc tcctccgccg tcttcacgat gatcttggcc atgccaatgc
     2461 agccgtcggt cggggccttg atcttgtaga ggagcagccg gtcggccacc gcgtcgatca
     2521 ggtccaggac gtcatcgagg cgactggcga gcgcgtagat gtcctcacgg tcgatgggcg
     2581 tgatgaacgt ggtgttgagc cggcggacga tctcgtgcgt cagggtgtcg cccgcgtgct
     2641 ccaggtcctt gatctgctgg gctttcgcct tggcgtccgc gtagtcgagg gtcaactcct
     2701 cgagcacgcg cgaggcggac acgatgtgcg tggcctgctg ctcgaacaga tcgaagaact
     2761 tctcttctcg cggaagcagc cggaacacgg gcgtatcgtc tcccggcgtc cggccgatgt
     2821 caacgccggc ggcccggttg acagggtggg accccctcgc taggatgacg cggtggcacg
     2881 ctcggagccc tgcgcccgcc gtcaggtgcg ctgcccgcgc tgtggcgcga gctgcggctg
     2941 gaccggtaat ccgcaccggc cgttctgctc gctcgagtgc cgcctgatcg atctgggggc
     3001 gtggctcgac gagcggtacc gtgtcccggg gccggagctg ccttccgact cggagtcccc
     3061 ggagcgcggg gctccgagcg gcggatgatc gacctcttcg acgatccgct cggcttcctc
     3121 tcccggctca tcctccagat ccccgctcta ctggtggcgg tgacggtgca cgaactcgcc
     3181 cacgcggtcg tggccgaccg tctgggtgac cccaccgccc ggcggctcgg gcgcctcacg
     3241 ctgaaccccc tgccgcatat cgatccggtc ggcgcgatcg cgttcgtgct cgcgggcttc
     3301 ggctgggcca agccggttcc ggtgaatgcg cagaacctgc gacaccccgt ccgggacatg
     3361 gcgtgggtcg ccgcggccgg ccccctgtcg aacttcaccg cggcgttcgt ggcgctcgtg
     3421 gcggtacggg tgttgggccg gctcgacggc gtcgcgccgt ccctcgtaag cgagcctctc
     3481 gccggggtcc tcttctggac ttacatcttc aacctggcgc tgggcatctt caacctgatc
     3541 ccgttgccgc cgctcgacgg cggacacttc ctgccctatc tgatcccgcg ggcctcatgg
     3601 agcttcatcc accagctcga acaggtgggc cctttcctgc tgatcctgct cctgctcagc
     3661 ggggcgacgc gttacgtcgt gcagccggtc ttcaatgcgg tgagcgcctt ctacctgtcg
     3721 atcgtccggc tcgtgatcta gcccgggcgc cgcggcgcgc cttcgcggga tccgcccctt
     3781 cgtgccggag tagccagcgc ttgcgccaga ggccgccgcc gtagccggtg agcgaggcgt
     3841 ccccgccgat cagccggtgg cacgggacga cgatggatat cggattgcgg ccgttcgcag
     3901 cgcccaccgc gcgcgccgcc gcgccgtgcc cgagcgcccg ggcgagctct ccgtagctcc
     3961 gggtgcggcc cggccggatc cggcgcagcg cctgccagac cttccgctgg aacggggttc
     4021 ccccggtgtc cactgggatg cgatcgaccg cggcgaggtc gccggccagg taggcgcgga
     4081 tcttccggct gatcccgaac gggtcccgcg tccacgtcgg ccgcgtaggg ccgtagcgcg
     4141 cggccacgga acggaccgcg cgggcgcgtc cgaactcgag ggcacacagg cgaccgtcgc
     4201 gggctgcgat cacgatgcgt ccgatcgggc tcacgatctc gtcgaagaga acacgatcgt
     4261 ccagtggagt acgcatgcgg ctcatgaccc cggacaggcc tgaacgtgaa agcgcccgag
     4321 cctttcggcc cgg
//
LOCUS       DBNGZW010000675         2302 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1905258, whole genome shotgun sequence.
ACCESSION   DBNGZW010000675 DBNGZW010000000
VERSION     DBNGZW010000675.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1905258"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1135
                     /gene="murD"
                     /locus_tag="AC16JC_15930"
     CDS             <1..1135
                     /gene="murD"
                     /locus_tag="AC16JC_15930"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR5166239.1"
                     /translation="AAGVVVSPGVPPDAPPLVAARAAGVPIVSELDLGFRGLSGVRCI
                     AVTGTNGKTTTTALIAHLLAQAGLRAEAAGNIGRPLADVALALEPPQWLAVEVSSFQL
                     HDSPHFAPEIGVLTNLAPDHLDRYPAVAAYYADKRIMFRNAMDDNVWVLNGDDPAALE
                     LGAAARGRHVRFSLRGPSDGWYDAAERSLRLGGGELLRREALQLLGLHNVANALAAAL
                     AVQEAGIGPAAIAEGLSTFRPLPHRLEPVGEVNGVRWINDSKATNVASTVVAIEAMDR
                     PFVLLLGGRHKGEPYTSLVPLLQPRCRLVVAYGEAGPLVEHDLAGRVPLERGGDFDDV
                     VARARRAARPGDAVLLSPACSSYDMFANYEERGAMFRRLALAA"
     gene            1132..2256
                     /locus_tag="AC16JC_15935"
     CDS             1132..2256
                     /locus_tag="AC16JC_15935"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR5166240.1"
                     /translation="MSERRWETRLIAVVAMGLSMIGIIAVYGASSLITVRGGGIVGST
                     FALNQAVGLLVGGLTATIIARLDYHLWERWAWHILGIVVLLLLVPLLPFTHAIAPMRN
                     GARRWVEIAGLSMQPSELAKLAIVAWTAMLAAKKGPDMRSFRRGVIPTLVIVAPVVGL
                     IFLEPNLSMALLVTLLAGVVMFSAGARIGHFLLLGVAAAPLAFGAVATAQYRISRVLT
                     FLNSGSAPSEATWQVHQSLVGIGAGQLLGVGIGHGQQKLGYLPYAYSDFIFSTLGEEW
                     GFLGVLLLAVGFGLFVWLGFRIARSARDPFGQLLAAGLTALIGIGAVLHIGVSLAVLP
                     ATGVTLPFVSYGRSSLLVSLIATGMLISVGQRRTGRAAGE"
BASE COUNT          274 a          806 c          830 g          392 t
ORIGIN      
        1 ggccgcgggc gtcgtggtgt ctcccggggt gccacccgat gcgccgccgc tcgtcgcggc
       61 gcgcgcggcg ggcgtgccga tcgtctccga gctcgatctc gggtttcgcg gtttgtccgg
      121 cgtgcggtgc atcgccgtca cggggacgaa cggaaagacg acgaccacgg cgctcatcgc
      181 ccacctgctc gctcaggccg gtctccgggc cgaggcggcc ggcaacatcg gacgaccgct
      241 ggcggatgtg gcgctcgcgc tcgagccgcc gcaatggctg gccgtggagg tctcctcgtt
      301 ccagctgcac gacagcccgc acttcgcgcc ggagatcggc gtgctgacca acctcgcccc
      361 cgaccacctc gaccggtatc ccgctgtcgc cgcctactat gcggacaaac gaatcatgtt
      421 tcgcaacgcg atggacgaca acgtgtgggt gctgaacggc gacgaccccg ccgcgctcga
      481 gctgggggcg gcggcgcgtg gccgtcacgt gcgattttcg ttgcgcgggc cgagcgacgg
      541 ctggtacgat gcggcggaac ggtccttgcg tctggggggt ggcgagctgc tgcgccgtga
      601 ggcgttgcag ctcctgggtc tgcataacgt cgcgaacgcg ctggcggccg cgctcgcggt
      661 gcaggaagcg ggcatcggtc cggcggcgat cgccgagggc ctcagcacgt tccggccgct
      721 gccccaccgc ctcgagccgg tgggagaggt gaacggtgtt cgctggatca acgattccaa
      781 ggcgacgaac gtcgcgtcca cggtggtggc gatcgaggcc atggaccggc cgttcgtgct
      841 gctgctgggc gggcgccaca agggcgagcc gtacacgagc ctcgtcccgc tgctgcagcc
      901 gcgctgccgc ctcgtcgtcg cctacgggga ggcggggccg ctggtggagc acgacctcgc
      961 cgggcgggtc ccgctcgaac gcgggggcga cttcgacgac gtggtcgccc gggcccgtcg
     1021 tgccgcccgt ccgggcgacg cggtgctgct ctccccggcg tgctcgagct acgacatgtt
     1081 cgccaactac gaggagcgcg gcgccatgtt ccggcggctc gcgctggccg cgtgagcgag
     1141 cggcgctggg agacgcggct catcgcggtg gtcgcgatgg ggctctcgat gatcgggatc
     1201 atcgcggtat acggcgcctc gagcctcatc acggtgcggg gcggcgggat cgtgggctcg
     1261 accttcgccc tgaaccaggc ggtgggactc ctggtcggcg gtctgaccgc gacgatcatc
     1321 gcccgcctcg actaccacct gtgggagcgg tgggcgtggc acatcctggg gatcgtcgtc
     1381 ctgctgctcc tcgtacccct gctgccgttc acccacgcca tcgcgccgat gcgcaacggg
     1441 gcgcgccgct gggtcgagat cgccgggctc tccatgcagc cttcggagct cgccaagctc
     1501 gccatcgtcg cgtggacggc gatgctcgcc gccaagaagg gccccgacat gcgcagcttc
     1561 cgccgcggtg tgatccccac gctcgtgatc gtggcgcccg tggtcgggct gatcttcctc
     1621 gagcccaacc tctccatggc gctgctggtc acgctgctcg ccggggtggt gatgttttct
     1681 gccggggcgc ggatcggcca cttcctgctg ctcggcgtcg ccgcggcgcc gctcgcgttc
     1741 ggggcggtcg ccaccgcgca gtaccgcatt tcgcgcgtgc tcaccttcct caattccggc
     1801 agcgcgccga gcgaagcgac ctggcaggtg catcagtcgc tcgtcggcat cggcgccgga
     1861 cagctgctgg gcgtcggcat cggccacggg cagcagaagc tcggctatct cccctacgcc
     1921 tactccgact tcatcttctc cacgctcggc gaggaatggg ggttcctcgg cgtgctgctg
     1981 ctcgccgtgg gcttcggcct gttcgtctgg ctcggcttcc gcatcgcgcg ctcggcccgc
     2041 gatccgttcg gccagctgct cgcggcgggg ctcaccgcgc tcatcggcat cggcgcggtc
     2101 ctgcacatcg gcgtgtccct ggccgtgctc ccggccaccg gggtcacgct ccccttcgtg
     2161 tcctacggac gatcgagcct gctcgtctcc ctcatcgcga cgggcatgct catcagcgtg
     2221 ggccagcgac gcaccgggcg ggcggcgggg gagtgaggca attgcggatt gtggattgcg
     2281 gatttcggac tggaaggagg ag
//
LOCUS       DBNGZW010000676         2896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_405097, whole genome shotgun sequence.
ACCESSION   DBNGZW010000676 DBNGZW010000000
VERSION     DBNGZW010000676.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_405097"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1051
                     /locus_tag="AC16JC_15940"
     CDS             <1..1051
                     /locus_tag="AC16JC_15940"
                     /inference="COORDINATES: protein motif:HMM:NF024116.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5166241.1"
                     /translation="VGGELSRGYDSAASQQAFYNALEERARALPGVAGVAEGVVAPFA
                     GSGVAYTSDFHLAGRPANDYGTEVGHDFVTPGYFQTLRIPLRAGRSFTPADRSGSVPV
                     TIINEALARRYFRGEDPVGQRITFDRIPDSTSVWRTIVGVVGDVRQRGLSVEPLIEAY
                     EPAAQNANSYMTLFVRTRDGGDAAALGPAIRGVITGLDRNLAPDLVRPLDRMQARSIA
                     DRRFLMSLLLVFAATGVLLALVGVYGVMAQLGRRRTREMGIRLALGARARQVQWLVVR
                     HGLQVVLLGLSLGVGGALAATRTIRVLLFEVPPRDPMTFVAVSMLLAFTATAASWLPA
                     LRASRADPARVLRVE"
     gene            1131..1925
                     /locus_tag="AC16JC_15945"
     CDS             1131..1925
                     /locus_tag="AC16JC_15945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166242.1"
                     /translation="MAERTASITSYRDFLAALLGNREQFFDEVVDGAGLGRKLAYSML
                     TVIALAGFFGCVAGAYSGPAQAISAGIKLPLLFFATFVICFPAFYVVQVLVGSRLRLP
                     QVVVLVFGALSLTSILLAAFVPVIAFFLLTGANYYFQHLLNIAIAGIAGAYGMYALHE
                     GLAVVCDKRGVYPRKALTIMRAWALLFAFVGVQLAWNLRPFLGDRNQPFRVIGSYQGN
                     FYAAVIYAVNKLFSGEDQPAPPRRPSVADSLPFTTAPVDTAYLRRP"
     gene            1922..2146
                     /locus_tag="AC16JC_15950"
     CDS             1922..2146
                     /locus_tag="AC16JC_15950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003141001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5166243.1"
                     /translation="MSGTPPEILTIDEVAAYLRLTPQTIYKWAQEKRIPAVKLGKEWR
                     FRRSVIDRWFDEQLLGNDSRFGHLKDGAPG"
     gene            2143..>2896
                     /locus_tag="AC16JC_15955"
     CDS             2143..>2896
                     /locus_tag="AC16JC_15955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166244.1"
                     /translation="MKRAAVLFLGMAAAGAAAHAQTLESPIRVALGFGVDTARSPERE
                     IFELYRHYLTHRQDTIRPHPDWSAAEQRRWPAFDLLGGYIYQGFSAFTVVHLAPAVGL
                     DSTFLIRTLISSVDDSTGAVRPLALYRVYAVREAGRWVLANALPRMTHGWHHDTIDSV
                     TFVFPPTRAFDRARAAAAARLVDSLARAFVLPAPQIDYYFTDDLIETFQALGLEFFPL
                     GADTVGGRSITADRLVLVGSSSRGEDYRHELAH"
BASE COUNT          396 a          986 c          998 g          516 t
ORIGIN      
        1 ggtcggcggt gagctttcgc gggggtacga cagtgccgcc agccagcagg cgttctacaa
       61 cgcgctcgag gagcgcgcgc gcgccctccc gggcgtggcg ggggtggccg aaggggtggt
      121 cgcgcccttc gcgggcagcg gggtggcgta caccagcgac ttccaccttg ccggccggcc
      181 ggcgaacgac tacggcaccg aggtggggca tgatttcgtg acgcccggct acttccagac
      241 gctgcggatt ccgctgcgcg cggggcgatc cttcacgccc gccgaccggt cggggtcggt
      301 accggtgacg atcatcaatg aagcgctggc gcgccgatat ttccggggag aagacccggt
      361 cggacagcgg attacgttcg acaggatccc cgattcgacg tcggtctggc gcaccatcgt
      421 cggagtcgtt ggggacgtgc gccagcgggg cctgtccgtc gaacccctga tcgaggcgta
      481 cgagccggcg gcgcagaacg ccaacagtta catgacgctg ttcgtgcgga cgcgggacgg
      541 aggcgacgcg gcggcgctcg ggcccgccat ccgtggggtc atcaccgggc tcgaccggaa
      601 cctggccccg gatctggtgc ggccgctgga ccgcatgcag gcacgctcca tcgcggaccg
      661 gcgcttcctc atgtccctgc tgctggtctt cgccgcgacg ggggtgctgc tcgccctggt
      721 cggcgtctac ggcgtgatgg cgcagctggg acggcggcgc acccgggaga tgggaatccg
      781 gctggcgttg ggcgcccgcg cgcgccaggt ccaatggctg gtggtccgtc acgggctcca
      841 ggtcgtgctg ctcgggctct cgttgggagt ggggggcgcg ctcgccgcga cacggaccat
      901 tcgcgtgctg ctcttcgaag tgccgccgcg ggacccgatg accttcgtgg ccgtctccat
      961 gctgctcgcg tttacggcga cggcggcgtc gtggctcccc gcgctgcggg ccagccgggc
     1021 tgacccggca agggtgctgc gggtcgagtg attcatcgcc aaacatcaat aaacttgacg
     1081 aaacattagt acgacagcat catagggggc atcccccatg gaggcccctt atggctgaac
     1141 gcaccgcctc gatcacgagc taccgggact tcctcgccgc cctcctgggc aaccgggaac
     1201 agttcttcga cgaggtcgtg gatggcgccg gactcggcag gaaactcgcg tacagcatgc
     1261 tgacggtcat cgcgctggcc ggcttcttcg gctgcgtggc cggggcttac tccggtccgg
     1321 cgcaggcgat ctcggcgggg atcaagctgc cgctgttgtt cttcgcgacg ttcgtgatct
     1381 gctttccggc gttctacgta gtgcaggtgc tcgtcgggtc gcggttgcgg ctgccgcagg
     1441 tggtcgtgct ggtgttcggc gcgctgtcgc tcacttcgat cctgctcgcg gcgttcgtgc
     1501 ccgtcatcgc gttcttcctg ctcaccggcg cgaactacta cttccagcac ctcctcaaca
     1561 tcgccatcgc gggcatcgcc ggcgcgtacg ggatgtacgc gctgcacgag gggctcgccg
     1621 tggtgtgcga caagcgcggc gtctatcccc ggaaggcgct cacgatcatg cgggcctggg
     1681 cgctgttgtt cgcattcgtc ggcgtgcaac tcgcctggaa cctccggccc ttcctgggcg
     1741 accgcaatca gccgttccgc gtcatcggga gctaccaggg caacttctac gcggccgtga
     1801 tctacgcggt caacaagctg ttcagcgggg aagatcagcc ggcgccgccg cgccgcccct
     1861 ccgtggccga cagcttgccc ttcacgaccg cgccggtcga taccgcttac ctgcgccggc
     1921 catgagcggc acacctcccg agatcctcac catcgacgag gtcgccgcct acctgcggct
     1981 cacgccgcag acgatctaca agtgggcgca ggagaagcgc atcccggccg tgaagctggg
     2041 gaaggagtgg cgcttccgcc gcagcgtcat cgaccgctgg ttcgacgagc agctgctggg
     2101 gaacgactct cgcttcggcc atctcaagga cggggcgccg gggtgaagcg ggccgcggtg
     2161 ctcttcctgg ggatggccgc cgccggcgcc gcggcccatg cccagaccct ggagtctccg
     2221 atccgggtcg ccctggggtt cggggtggac accgcccgct cgcccgaacg ggagatcttc
     2281 gagttgtacc ggcattacct cacgcatcgt caggacacga tccgcccgca tcccgattgg
     2341 tcggcggccg agcagcggcg ttggccggcc ttcgatctgc tgggcggcta catctaccag
     2401 ggcttctccg cgtttacggt cgtgcatctc gctcccgcgg tcggcctcga cagcacgttc
     2461 ctcatccgca ctctgatctc gtcggtggac gattcgacgg gggcggtccg gccgctggcg
     2521 ctgtatcggg tctacgctgt gcgcgaggcg gggcgctggg tactcgccaa tgccctgcca
     2581 cggatgacgc acggctggca tcacgacacg atcgacagcg tgaccttcgt ctttccaccg
     2641 acccgggcgt tcgaccgcgc acgcgccgcc gccgccgccc gattggtcga ttcgctggct
     2701 cgcgcgttcg tcctgccggc gccgcagatc gactactact tcaccgacga cctgatcgag
     2761 acattccagg cgctcggtct cgagttcttc ccgctggggg ccgacacggt gggcggccgc
     2821 tcgattacgg ccgaccggct ggtgcttgtc ggctcatcgt cgaggggaga ggactatcgc
     2881 cacgagcttg cacatg
//
LOCUS       DBNGZW010000677         2452 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1563985, whole genome shotgun sequence.
ACCESSION   DBNGZW010000677 DBNGZW010000000
VERSION     DBNGZW010000677.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2452)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1563985"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..612
                     /locus_tag="AC16JC_15960"
     CDS             100..612
                     /locus_tag="AC16JC_15960"
                     /inference="COORDINATES: protein motif:HMM:NF021505.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HIR5166245.1"
                     /translation="MIPNPLHPSLVHFPIVFAVLLPLVAIGALWMIHRGAAARRAWLL
                     PVALAGALTLSAFLAVQTGKAQEERVEDVVGRQPLHSHEESAELFMVLSGVLVVVTAV
                     GLAPGLAGRAARIVATLGAFGLLGAGYTVGRSGGELVYQHGAASAYASQHGPGGIVPA
                     RSAEADEEGK"
     gene            629..1297
                     /locus_tag="AC16JC_15965"
     CDS             629..1297
                     /locus_tag="AC16JC_15965"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR5166246.1"
                     /translation="MSGAPAFLFDLDGTLVDSVYQHVLAWREALERVGVPLSVWRIHR
                     RIGMSGGLLVNAFLRETGREMSPEEAEQVRRFHAQAYARMAGTVRPLPGARDLLAHLT
                     RLRVPWAVATSGWIESARPTLEMLGVPSGVPVVTRDQVEHAKPDPDPFLAAAERLGIP
                     ITESVVVGDSVWDLLAAQRARALGIGLLSGGYGQDELERSGAYRVYQDPADMLRHLDE
                     VGVR"
     gene            1383..>2452
                     /gene="hemA"
                     /locus_tag="AC16JC_15970"
     CDS             1383..>2452
                     /gene="hemA"
                     /locus_tag="AC16JC_15970"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HIR5166247.1"
                     /translation="MPLACTGINHRTAPLSLRERLALGPEAQRDMLRAPEVRSLGSQV
                     GLGELVILSTCNRTELYAAPVDGASRSRDISDSLLQLLAAISGLPLPTLRPHTYGDTG
                     LAALRHLCRVAAGLDSMVVGESEVLGQVATAFQTAASEGATGPVLEAAFRAATRAGRR
                     ARTETGICRHPASVSSEAVKLLAEVAGDLEGLAVLVVGTGKMGRLAGQALLGSGVRQL
                     CVISRTAAHAEAVARDWGGATALGWHDLEAAVRAADAILCSTGAPHAVVTREMVERAI
                     GPAGDGRRRCFVDIAVPRDVEPEVARLPGVQVFDLDALQERLRDNLDLRRREIPAVER
                     IVSEEVQHFETWRQGMTVRPLLT"
BASE COUNT          324 a          853 c          908 g          367 t
ORIGIN      
        1 tggaggcgcg aaattgcccc gcgaggctgt cgaacgtcgg ataccgcgcc gcggccgccg
       61 gctccatgtt gcgcgctcaa ccagacagag gatacccgta tgattcctaa cccgctgcac
      121 ccgtcgctgg tgcatttccc gatcgtgttc gcggtgctgt tgccgctcgt ggcgatcggc
      181 gcgttgtgga tgatccatcg aggcgctgcc gcccgccgcg cctggttgct ccccgtcgcc
      241 ctggccggcg ccttgacgct gtccgcgttc ctcgccgtgc agaccggcaa ggcccaggaa
      301 gaacgcgtcg aggacgtcgt cggccgacaa ccgctgcact cgcacgaaga atccgccgag
      361 ctgttcatgg tgctgtccgg cgtgctggtc gtcgtgacgg ccgtgggcct cgcccccggc
      421 ttggccggac gcgccgcacg catcgtcgcc acgctcggcg cgttcggcct gctcggcgcg
      481 ggctacaccg tcgggcgctc gggcggcgag ctggtctatc agcacggtgc ggcgagcgcg
      541 tacgcgagcc agcacggacc cggtgggatc gtgccggcac gttctgccga ggccgacgag
      601 gaggggaaat agccggtaga ttcccgggat gagcggggct ccggcattcc tgttcgatct
      661 cgacggcacg ctggtagaca gcgtgtacca gcacgtgctc gcctggcgcg aggcgctgga
      721 gcgggtcggg gttccgttgt cggtgtggcg catccaccgg cgcatcggca tgagcggcgg
      781 gctgctggtg aacgcgttcc tgcgggaaac gggacgcgag atgagcccgg aggaggcgga
      841 gcaggtgcgc cggttccacg cccaggccta cgcgcggatg gcggggacgg tccggccgct
      901 gcccggcgcc cgcgacctgc tggcgcacct gacccgtctc cgcgtcccct gggcggtcgc
      961 caccagcggc tggatcgaga gcgcccggcc gaccctggag atgctcggcg tgccttccgg
     1021 agttcccgtc gtcacgcgcg accaggtgga gcacgccaag cccgaccccg accccttcct
     1081 cgcagcggcc gagcggctgg ggattcctat cacggaatcg gtggtcgtcg gggacagtgt
     1141 ctgggacctg ctcgccgcac agcgggcccg ggcgctcggt atcggactcc tgtcgggcgg
     1201 ctacgggcag gacgagctgg agcgctctgg ggcttaccgg gtgtaccagg acccggccga
     1261 catgctgcgg cacctcgacg aggtgggagt ccgatgaagc cggcatgaag ccggtctgac
     1321 gtacaactac ggaggaaacc ctccgaatag tgcttcatga aggccggctc tagcatgcgg
     1381 gcatgccgct cgcgtgcacc ggcatcaacc accgcaccgc accgctctcc ctgcgtgagc
     1441 ggctggccct cggtcccgag gcgcaacggg acatgctgcg ggcccccgag gtccgcagcc
     1501 tcggatcaca ggtcggtctt ggcgagctgg tcatcctctc gacctgcaat cgcaccgagc
     1561 tgtatgccgc tccggtggac ggtgcgtccc ggtcccgcga catctccgat tcgctgctgc
     1621 agctgctcgc ggcgatctcc ggcctgccgc tccccacgtt gcgcccgcac acctacggcg
     1681 acaccggcct ggccgcgctg cgccacctgt gtcgcgtcgc cgcggggctc gactcgatgg
     1741 tggtcggcga atccgaggtg ctgggccagg tggcgacggc gttccagacg gcggccagtg
     1801 aaggcgcgac cggaccggtg ctcgaagccg cgttccgcgc cgcgacccgc gccgggcggc
     1861 gggcacgcac cgagacggga atctgccgcc atccggccag tgtgagctcc gaggcggtca
     1921 agttgctggc ggaggtcgcc ggcgacctcg agggcctggc ggtgctcgtg gtcggcacgg
     1981 gcaagatggg ccggctcgcg ggacaggcgc tgctcgggag cggcgtgcgg cagctctgcg
     2041 tgatcagccg caccgcggcc cacgcagagg cggtcgcccg ggactggggc ggcgcgacgg
     2101 cgctcggctg gcacgatctc gaagccgcgg tgcgggccgc cgacgcgatc ctctgctcca
     2161 ccggggcgcc gcacgcggtc gtgacgcgcg aaatggtgga gcgcgcgatc gggccggcgg
     2221 gcgacggccg ccggcgctgc ttcgtggaca tcgcggtgcc gcgcgacgtg gagcccgagg
     2281 tcgcccggct tcccggcgtc caggtgttcg acctcgatgc gctgcaggag cggctccgcg
     2341 acaacctgga tctgcggcgc cgcgagatcc ccgccgtcga gcgcatcgtg tcggaagagg
     2401 tgcagcactt cgagacgtgg cggcagggga tgaccgtgcg gccgctcctg ac
//
LOCUS       DBNGZW010000678         4685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1820041, whole genome shotgun sequence.
ACCESSION   DBNGZW010000678 DBNGZW010000000
VERSION     DBNGZW010000678.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1820041"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..726)
                     /locus_tag="AC16JC_15975"
     CDS             complement(<1..726)
                     /locus_tag="AC16JC_15975"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5166248.1"
                     /translation="MTTAVEHPAGGLSDTQRQIVDLAREFAHEHIAPNAAEWDRTREF
                     PRAVIDELGRLGFLGMTVPERYDGMGIDTVTYLLALEEIAAADASIAVSISIQNAIPA
                     TMLVRHGSPAQQERWLKPLARGELLVGFALSEPDAGSDPSSMRAQAVRDGDGWVLNGM
                     KAWATNGGTADLMMVMVRTDSGSDRRGAKGISTFIVPTATPGYRAGAPEDKMGLRASN
                     TTTVALEDLRLPADALLGEEGMGF"
     gene            complement(710..1561)
                     /locus_tag="AC16JC_15980"
     CDS             complement(710..1561)
                     /locus_tag="AC16JC_15980"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013822331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - (3S)-3-hydroxyacyl-CoA
                     dehydrogenase (NAD+) activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5166249.1"
                     /translation="MSRVAVVGAGTMGSGIAHVFAQHGWETLLLDTAPGALDQARQTI
                     AANLDRQVKKGALTAEQKAAALDRLVTAPALDGKVGGPELVIEAATEQRETKFAIFRE
                     LDRAAPPEAILATNTSSISITAIAAQTSRPGLVIGMHFMNPVPVMPLVEIIRGLDTTD
                     ATTGRTVAIAQALGKTPVEVRDAPGFVSNRVLMPMINEAIFCVMEGVATPDAVDTVMK
                     LGMNHPLGPLALADLIGLDVCLAILEVLHRDLGDDKYRPCPLLRTMVAAGLLGRKSGR
                     GFFDYGR"
     gene            complement(1558..2427)
                     /locus_tag="AC16JC_15985"
     CDS             complement(1558..2427)
                     /locus_tag="AC16JC_15985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR5166250.1"
                     /translation="MARVSFVARLRELGRAVLWLTLFAVVGIGVMVVLSNAIPGVGGP
                     RWALARGGLYELGGFSLGTWLVGRLANGYSWDRMGWRSRRTVPRHFLHGLAFGAAMAA
                     VAVLLAVLIDGAALRLSGVEGFPAVAGPLLIGLCAAALSEELMWRGYPLRRLADAIGP
                     GTATLVLAIGFGAAHLGNEGMNLLSTVNIAVAGLWLGVAFFSRGGMALAWGLHLGWNA
                     GLSLVFDAPVSGQAFNVPGMDYMPGAHAWLDGGPFGPEGGLIGTLVFAGGMVLLLGRE
                     IRRPRAWLAEAAA"
     gene            complement(2427..2987)
                     /locus_tag="AC16JC_15990"
     CDS             complement(2427..2987)
                     /locus_tag="AC16JC_15990"
                     /inference="COORDINATES: protein motif:HMM:NF014669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR5166251.1"
                     /translation="MDRAIVLPSPVLTWRRTSAVVLGAALVAAAAQIAVPLPGTPVPL
                     TLQPLAVLIVGGLLGPWLGASSLTFYLALGAAGFPVFTPLGLPGLARLIGPTGGYLLA
                     YPLAAYAVGALSRGAPGYARLALATFAGLVLIHLGGLVQLWVYTGNVRDAARYGTLPF
                     VVGDLIKLGVAVLLLHPTLRPVRARL"
     gene            complement(2988..4178)
                     /locus_tag="AC16JC_15995"
     CDS             complement(2988..4178)
                     /locus_tag="AC16JC_15995"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR5166252.1"
                     /translation="MATDQTTPVIVSACRTPIGRFLGGLSPLTAPKLGALVVKEAVRR
                     AGIDAGAVDEVILGNVLQGGEGQAPARQAAIHAGLPGTIPSLTINKVCGSGLKAVMLA
                     AQAVKAGDAQCVVAGGMESMSNAPFYLYGMRGGVKAGNQPLTDGMIHDGLWDSFSNQH
                     MGNLAEYTAKKAGVSRLDQDAFALASHRKAVAAMEACHFKAETVAVEIAGKKGPPVID
                     KDEGPRADTSLEALAALKPSCEKDGTVTAGNAPGLNDGASALVVTSLAFARTHGLQPL
                     ARITAYATGGGEPKDLFFAPIVAVQNLMQQAGTTIGDYDLIEANEAFAVQALADGRAL
                     GWDWDRVNVNGGAIALGHPIGASGARVLTTLLYAMKDRGRRTGLATLCLGGGNAVALS
                     VEAM"
     gene            complement(4215..>4685)
                     /locus_tag="AC16JC_16000"
     CDS             complement(4215..>4685)
                     /locus_tag="AC16JC_16000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5166253.1"
                     /translation="FNLPERFALEYTGADNKPHRPVMIHRAIFGTMERFVGFLIEHFA
                     GAFPLWLAPEQVRVLPIADAQLEAARGVADTLRRAGLRVQLDERSDTLNYKIRDGEMQ
                     KIPYLAVVGQREAEAGAVAMRARGAGKKQTVLGIDEAVRRLTEEVRTRSLAPLI"
BASE COUNT          725 a         1787 c         1536 g          637 t
ORIGIN      
        1 gaaccccatc ccctcctcgc ccagcagggc gtcggcgggg aggcgcaggt cctcgagcgc
       61 gaccgtggtc gtgttggacg cgcgcaggcc cattttgtcc tccggcgcgc ccgcccgata
      121 gcccggcgtc gcggtgggga cgatgaacgt cgagatcccc ttggcgccgc ggcggtcgga
      181 gcccgagtcg gtccggacca tgaccatcat gagatcggcc gtgcccccgt tggtcgccca
      241 cgccttcatg ccgttcagca cccagccatc gccgtcccgc accgcctgcg cccgcatcga
      301 cgagggatcg gagccggcat ccggctccga cagcgcgaat ccgacgagca actcgccgcg
      361 cgccagcggc ttcagccagc gctcctgctg cgcagggctg ccgtgccgca ccagcatcgt
      421 ggcggggatc gcgttctgaa tgctgatcga cacggcgatc gatgcgtcgg ccgcggcgat
      481 ttcctcgagc gccaggagat aggtcacggt gtcgatcccc atgccgtcgt agcgctcggg
      541 gacggtcatg ccgaggaaac cgagccggcc cagctcgtcg atcacggcac gcggaaactc
      601 ccgcgtgcga tcccactccg ccgcgttcgg cgcgatgtgc tcgtgcgcga actcgcgcgc
      661 cagatccacg atctggcgct gcgtatcgct cagcccgccg gcgggatgct caacggccgt
      721 agtcaaagaa ccccctcccg ctcttgcggc ccaggaggcc ggccgccacc atcgtgcgca
      781 gcagcgggca cggccggtac ttgtcgtctc cgagatcgcg gtgcagcacc tcgaggatcg
      841 cgaggcagac gtcgaggccg atcaggtcgg cgagcgccag cggcccgagc ggatgattca
      901 tgccgagctt catcaccgta tccaccgcgt ccggcgtcgc gacgccttcc atcacgcaga
      961 agatcgcctc gttgatcatc ggcatgagga cccggttcga cacgaacccg ggcgcgtcgc
     1021 gcacttccac cggcgtcttg cccagcgcct gcgcgatcgc gaccgtccgg ccggtggtcg
     1081 cgtccgtggt gtcgagcccc cggatgatct ccaccagcgg catcaccggc accgggttca
     1141 tgaagtgcat gccgatcacg aggcccggac gcgacgtctg ggcggcgatc gcggtgatcg
     1201 agatggacga ggtattggtg gcgagaatcg cctccggcgg cgccgcccgg tcgagctcgc
     1261 ggaagatcgc gaacttcgtc tcgcgctgct ccgtggccgc ttcgatcacc agctcgggcc
     1321 cgcccacctt cccgtcgagc gccggcgccg tcacgagccg gtcgagcgcc gccgccttct
     1381 gctccgcggt gagcgccccc ttcttgacct ggcggtcgag gttggcggcg atcgtctggc
     1441 gcgcctggtc gagcgctccc ggggccgtgt ccagcagcag cgtctcccac ccatgctgcg
     1501 cgaacacgtg ggcgatcccg ctccccatcg tgcccgcgcc caccaccgcg acccggctca
     1561 cgcggcagcc tcggccagcc aggcccgggg gcggcggatt tcccgtccca acaggagcac
     1621 catcccgccg gcgaacacca gcgtcccgat cagcccgccc tcgggcccga acgggccccc
     1681 gtcgagccag gcatgcgccc cgggcatgta gtccatgccc ggcacgttga aggcctggcc
     1741 ggacaccggg gcgtcgaaca ccaggctcag cccggcgttc cagcccagat gcaaccccca
     1801 cgcgagggcc atcccgccgc gcgaaaagaa cgccaccccc agccacaacc ccgcgaccgc
     1861 gatgttgacc gtggagagca ggttcatgcc ttcgtttccc agatgggccg caccgaaccc
     1921 gatggccagc accagcgtcg ccgtgcccgg cccgatcgca tccgcgagcc gtcgcagcgg
     1981 gtagccgcgc cacatcagct cttcggagag cgcggccgcg cacagcccga tgagcagcgg
     2041 gccggcgacg gctggaaacc cctccacgcc ggaaagccgc agcgcggcgc cgtcgatcag
     2101 cacggcgagc agcaccgcca ccgccgccat cgccgcgccg aacgccaacc cgtgcaggaa
     2161 atgtcgcggc accgtgcgcc gcgaccgcca tcccatgcgg tcccaggagt agccgttcgc
     2221 caaccggccc acgagccagg tgccgagcga gaatccgccc agctcgtaca gcccgccgcg
     2281 cgccagcgcc caccgcgggc cgccgacgcc gggaatcgcg ttgctcaata ccaccatcac
     2341 gccgattccc acgacggcga agagcgtcag ccagagaacc gcgcggccga gctcccgcaa
     2401 tcgggccaca aacgaaacgc gcgccatcag aggcgcgcgc gaaccgggcg aagggtcggg
     2461 tgaagcagca gcacagcgac tccgagtttg atcaggtcac ccaccacgaa cggcagcgtg
     2521 ccgtagcggg ccgcgtcgcg gacgttcccg gtgtacaccc acagctgcac caggccgccc
     2581 agatggatca acacgagtcc ggcgaacgtc gccagggcga ggcgcgcgta ccccggcgcc
     2641 ccacgcgaca gcgcgcccac ggcgtacgcc gcgagcgggt aagccagcag gtagccgccc
     2701 gtgggcccga tgagccgggc cagtccgggc agtcccagcg gagtgaacac ggggaagccc
     2761 gccgcgccga gggcgagata gaacgtcagg ctcgacgcgc ccagccacgg ccccagcagt
     2821 cccccgacga tcagcaccgc gaggggctgg agcgtcagcg gcaccggtgt cccgggcagc
     2881 gggacggcga tctgcgcggc ggccgccacc agcgcggcgc ccagcaccac ggcggacgtg
     2941 cgccgccagg tgagcacggg actcggcaat acgatcgctc ggtccattta catggcctcc
     3001 acgctgagag cgacggcgtt gccgccgccg agacagaggg tcgcgaggcc ggtgcgccgg
     3061 ccccggtcct tcatggcgta cagcagcgtc gtcagcacgc gcgcgcccga cgccccgatc
     3121 gggtgcccca gcgcgatggc gccgccgttc acgttgacgc ggtcccagtc ccagccgagc
     3181 gcccgcccgt cggcgagcgc ctggaccgcg aacgcttcgt tcgcctcgat caggtcatag
     3241 tcgccgatcg tggtgccggc ctgctgcatc aggttctgga ccgcgacgat cggtgcgaag
     3301 aagagatcct tcggctctcc gcccccggtg gcgtaggcgg tgatgcgcgc cagcggctgg
     3361 aggccgtgcg tccgggcgaa ggcgagcgac gtcacgacca gcgcggacgc cccgtcgttc
     3421 aatcccggcg cgttgccggc cgtcacggtg ccgtccttct cgcacgacgg cttgagcgcg
     3481 gccagcgcct cgaggctcgt atcggcgcgc gggccctcgt ccttgtcgat gacggggggc
     3541 cccttcttcc ccgcgatctc cacggccacg gtctcggcct tgaagtggca cgcctccatc
     3601 gcggccaccg ccttgcggtg gctcgccagg gcgaaggcat cctgatccaa ccgcgacacg
     3661 ccggccttct tcgccgtgta ctcggcgaga ttccccatgt gttgattcga gaacgagtcc
     3721 cacagaccgt cgtggatcat gccgtcggtg agcggctgat tgcccgcctt caccccgccc
     3781 cgcatcccat acagatagaa aggggcgttc gacatcgatt ccatcccgcc cgccaccacg
     3841 cactgcgcgt cgccggcctt caccgcctgc gccgccagca tcacggcctt gagccccgag
     3901 ccgcagacct tgttgatggt gaggctcggg atcgtgccgg gcagccccgc atggatcgcc
     3961 gcctgccgcg ccggggcctg cccctcgccg ccctgcagca cgttgcccag gatgacctcg
     4021 tccaccgccc cggcgtcgat gccggcgcgg cgcacggctt ccttcaccac cagggcgccg
     4081 agcttcggcg ccgtgagggg cgacaggccg cccaggaacc ggccgatcgg cgtgcgacag
     4141 gcggagacga taaccggagt tgtctgatcg gtagccatga tccgttcctc aatccgaaat
     4201 ccacaatccg caactcatat taacggcgcg agggagcggg tgcgaacctc ctcggtcagg
     4261 cgccgcaccg cttcatctat ccctaacact gtctgcttct tgccggctcc ccgggcccgc
     4321 atcgccaccg cgccggcctc ggcctcccgc tgccccacga ccgcgagata ggggatcttc
     4381 tgcatctccc cgtcgcggat cttgtagttc agggtgtcgc tgcgctcgtc gagctggacc
     4441 cgcagtcccg cgcggcgcag cgtatcggcc acgccgcgcg ccgcctcgag ctgcgcgtcg
     4501 gcgatcggca ggacgcgcac ctgctccggc gccagccaca gcgggaacgc cccggcgaag
     4561 tgctcgatca ggaagccgac gaaccgctcc atcgtgccga agatggcccg gtggatcatc
     4621 accgggcggt ggggcttgtt gtcggcgccg gtgtactcca acgcgaaccg ctccggcagg
     4681 ttgaa
//
LOCUS       DBNGZW010000679         9886 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_880571, whole genome shotgun sequence.
ACCESSION   DBNGZW010000679 DBNGZW010000000
VERSION     DBNGZW010000679.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_880571"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(76..1995)
                     /locus_tag="AC16JC_16005"
     CDS             complement(76..1995)
                     /locus_tag="AC16JC_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011421689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HIR5166254.1"
                     /translation="MSVELRANVPDGPIAQKWERHRFEMKLVNPANKRKFEVIVVGSG
                     LAGASAAASLAELGYGVSCFCFQDSPRRAHSIAAQGGINAAKNYQNDGDSVWRLFYDT
                     VKGGDFRSREANVYRLAQISVDIIDQCVAQGVPFAREYGGLLANRSFGGAQVSRTFYA
                     RGQTGQQLLLGAYQALSRQIGLGNVRMYPRTEMLDLVVVDGKARGIVVRDLVTGRISS
                     HAAHAVVLATGGYANVYYLSTNAKGCNVTATWRAARRGAFFANPCFTQIHPTCIPAHG
                     VHQSKLTLMSESLRNDGRIWVPQQQGDTRQAGAIPAGERDYYLERMYPSFGNLVPRDV
                     ASRRAKEMCDAGHSVVPNGHGVYLDFHDAIERLGRQTIAERYGNLFEMYERITGENPY
                     EVPMRIYPAPHYTMGGLWVDYNLMSNLEGLHVVGEANFSDHGANRLGASALMQGLADG
                     YFILPYTIGDYLARTALPPLDEGHAAFQQVEAEVRERTGRLLGVNGATSVDAFHRALG
                     RLLWDRCGMSRRREGLEQALGAIQELRERFWTDVFVGGRGEDLNQALERAGRVADFME
                     FAELMCEDALHREESCGGHFREEYQTPDGEAKRDDARFSYAAAWEWKGAGTAPQLHQE
                     PLAFEHVHLAQRSYK"
     gene            complement(1992..2669)
                     /locus_tag="AC16JC_16010"
     CDS             complement(1992..2669)
                     /locus_tag="AC16JC_16010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HIR5166255.1"
                     /translation="MSRVTALTHSLIGKKVVMAVSGIVLLGYVLGHMAGNLKIFQGPE
                     HFNAYAEGLRTVGAPFFGRGQLLWIVRIFLLVAVGAHIWAAWVVTRASWAARPAGYHA
                     LRLVETTYAARTMRWGAVIILAFVTFHLLDLTFGPANPAFIPGDAYHNVIATFQRAPV
                     AVGYLVAMGTLCLHMYHGIYSGCQTLGLNHPKYNAARRGFALIFSLVVAGGFALVPLA
                     VLSGVVR"
     gene            2899..4221
                     /locus_tag="AC16JC_16015"
     CDS             2899..4221
                     /locus_tag="AC16JC_16015"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014433094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003954 - NADH dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006116 - obsolete NADH oxidation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5166256.1"
                     /translation="MSSESTLTGTEPTAKTADLAAVTPAPSARPRVVIVGAGFGGLRA
                     ARALRRTPVDVLLVDRNNYHLFQPLLYQVATAGLEPEQIAKPVRAILRGQRNFDFRLA
                     DVSGVDFAGRRLVTGDGAIAYDYLILATGAQTNFFGLTGVAQHALTLKDIPDAVRIRN
                     HVLACFERAMFEPDAERRRALLSFVVVGGGPTGVEMAGSLSELIRLVLVKDYPRLNLK
                     DVRILLLEAGDRLLAAFTPRLSEAAAATLWKKHVEVRHGAMVVDFDGAAARLKSGEVI
                     PARTLIWAAGARAAPLAGQLGIPTAHLGRVVVQPTLQLAGHPEVFVIGDSAFLEAQGP
                     SLPMMAPVAIQMGETAAANIRRALAGRTPEDFRYRDPGTLATIGRNAAVARIHGVSFK
                     GFPAWVVWLVVHLIQLIGFRNRLFVLTNWAWDYFFYERAVRLITEERH"
     gene            complement(4336..4605)
                     /locus_tag="AC16JC_16020"
     CDS             complement(4336..4605)
                     /locus_tag="AC16JC_16020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166257.1"
                     /translation="MGLSFIYNRQSVTQGTVTQTIKGYGAGVFVNLGGTWLVTPHLGL
                     GAQWQVDVTYSHNSNSAPFPNPSPGTTDIVTVELAHVALTGQFYF"
     gene            5158..7659
                     /locus_tag="AC16JC_16025"
     CDS             5158..7659
                     /locus_tag="AC16JC_16025"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF027637.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HIR5166258.1"
                     /translation="MRPPSYAVVLALTLAACGKAAPAAQTPAPVPGGGGAAPPASARQ
                     DTTRARPGQAAEGPKPYAQVITKEATTDSGVFLVHRIGEKLFYEIPRGQLDKEFLLVI
                     DYAGTPEGTRYGGEELDSRVVRWERMANRVLLRGKSYDIVADSSTTVSRAVRLSTIEP
                     VLMSFDVAAFSPKDSNLVIEVTKLYTTDVPEFNTRRVFRLRRLDPARSLVESARSFPT
                     NIEVRALHTFEGDSVPGNDRGLGTATFEMHYSMVLLPERPMARRLCDNRVGFFAVRQI
                     DYGLDRPVVTTRCYITRWRLEPKDPGAAVSDPIKPIVFYLDPATPERWAPWIIKGVQA
                     WEPVFRAAGFSNAITARRAPTPQEDPEFDLDDARYSTIRWLPSQIENAYGPHTSDPRT
                     GEILQSNIGWFQNTTSLLQAWYWVQAGAVDPRARQLPLPDSMMGQMVAYVATHEVGHT
                     LGLPHNMLSSGLYPVDSLRSPTYTRRNGTSYSIMDYARNNYVAQPGDDVQLMPTLGPW
                     DYYVIDWGYRRMPGAATPDVERPLLDSLARLQDASPWLQFGNPDGIDPRTQTEALGDD
                     PVKATQYGLANIKRLVPMLLPATTTETLEDYSLLDDLYDRLIAQWGLEMNHVAVVPGG
                     VWRHEKYPDQSGVIHQPVPRARQAEAVRFLNENAFATPRYFLDTAVLRRIEPTGFVER
                     IRTRQTALLNTLLQDSRLSRLAEQGATMPAGTAYTLTDLLDDLRSGIFSEVGAARPSV
                     DAYRRNLQQAFVDQMNRLINTPLVTPLPPGFFGFPGFTPPPPRPADARSVARLELREL
                     QTTLGTAAGRAADRTTRAHFLDLQARIGQILNPPR"
     gene            7758..8417
                     /locus_tag="AC16JC_16030"
     CDS             7758..8417
                     /locus_tag="AC16JC_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015040016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HIR5166259.1"
                     /translation="MLSTSTSHRESRFTRRPLVLALTGWYALVWVVTAIRPFDRHDWL
                     LENILVVLVLAVLVGSYRVFPLSDLSYLLIAAFMTLHASGAHWTYAQVPFGFWLQQVL
                     GLARNPFDRLVHLCFGLWLAYPIREVFLRVANARGVWAYYLPLDVTLAFSALYEIMEM
                     VVATVVAPGTGDAWLGTQGDVWDAQKDMAMAASGALLCMLITATVRRLRRRPPVLLAG
                     G"
     gene            8527..>9886
                     /locus_tag="AC16JC_16035"
     CDS             8527..>9886
                     /locus_tag="AC16JC_16035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR5166260.1"
                     /translation="MRPVHALIAGLALAFASAPLHAQQIATVELSPAHASVTAGDTIR
                     FRVTARDSAGGVVTGAHTEWFATPFDIAAADANGLVTTTRAGQTYVFALVGGKPAFAT
                     LDIVERPAARLVIAAPGGRTSLVVGEVVPLVAHAVTAVGDPVGEGRGLPGVQWRSLGP
                     AVAAVSPGGVVRARAVGRATIAAALGSVSARWEVTVRRNPVTSLRIRSVGPTRVGDVV
                     ALSPDARGAGGRSESDVPLRWAVTGPGAAVYPDGRFVATEPGMYTVSATVGSRVATVE
                     VPVLPRHDPRGVERVSHVILPKDAQGAEIWPVGDVAYVSTIGSAVYVFDIADPAAPRL
                     TDSLVVDARLLNDLMTTADGKILVVSREGASTRKNGLLFYDCADPRHPKLVSEFTETV
                     SGGVHSAYVYQHYVFATDDATGSLRIIDFADPRAPKQIARWEVPRAIPAQPYPVEFLN
                     IIP"
BASE COUNT         1517 a         3389 c         3433 g         1547 t
ORIGIN      
        1 ccgtcgcctc gtagcgcacg aacttgcccg gagccgcggg gccggcctgc cgccagacgt
       61 ggagtacgag gcgcatcact tgtagctccg ctgggcgagg tggacgtgct cgaaggcgag
      121 cggctcctgg tgcagctggg gagcggtgcc cgcgcccttc cactcccagg cggccgcgta
      181 ggagaaccgg gcgtcatccc gcttcgcctc gccgtcgggc gtctggtact cctcgcggaa
      241 atgaccgccg caggactcct cgcggtgcag ggcgtcctcg cacatcagct cggcgaactc
      301 catgaagtcg gccacgcggc cggcgcgttc cagcgcctga ttcaggtcct caccccggcc
      361 gccgacgaag acgtcggtcc agaagcgctc gcgcagctcc tggatcgcgc cgagggcctg
      421 ctccaggccc tcgcggcgcc gcgacatgcc gcagcggtcc cagagcaggc ggcccagcgc
      481 gcggtggaac gcgtccacgc tcgtcgcgcc gttcacgccg agcagccggc cggtccgctc
      541 gcgtacttcc gcttccacct gctggaaggc ggcgtgcccc tcgtccagcg gcggcagcgc
      601 cgtgcgcgcg aggtagtcgc cgatggtgta ggggaggatg aagtatccgt cggccaggcc
      661 ctgcatcagg gccgacgcgc cgaggcggtt ggcgccgtga tcggagaagt tggcctcgcc
      721 gaccacatga aggccttcga ggttcgacat caggttgtag tccacccaca gcccgcccat
      781 cgtgtagtgc ggggccgggt agatgcgcat cgggacctcg taggggttct ccccggtgat
      841 gcgctcgtac atctcgaaca ggttgccgta gcgctcggcg atggtctgcc ggcccagacg
      901 ctcgatcgcg tcgtggaaat cgaggtagac gccgtgtccg ttcggcacga cgctgtgccc
      961 cgcgtcgcac atctccttgg cgcggcgcga tgcgacgtcg cgcggcacca ggttgccgaa
     1021 gctcggatac atccgctcca ggtagtagtc ccgctcgccc gcggggatcg cgcccgcctg
     1081 ccgcgtgtca ccctgctgct gcgggaccca gatgcgcccg tcgttgcgca gcgactcgct
     1141 catcagcgtc agcttcgact ggtgcacgcc gtgcgccggg atgcaggtgg ggtggatctg
     1201 ggtgaagcac gggttcgcga agaacgcccc ccgccgggcc gcgcgccagg tggcggtgac
     1261 gttgcacccc ttggcgttgg tggagaggta gtagacgttg gcgtagccgc cggtcgcgag
     1321 caccacggcg tgggccgcgt gggagctgat ccggccggtg acgaggtcgc gcaccacgat
     1381 gccgcgggcc ttgccgtcga ccaccaccag gtcgagcatc tcggtgcgcg ggtacatgcg
     1441 gacgttgccg agcccgatct ggcgcgacag cgcctgatag gcgccgagca gcagctgctg
     1501 gcccgtctgg ccgcgggcgt agaaggtccg cgagacctgc gcgccgccga aggagcggtt
     1561 ggccagcagg ccgccgtatt cgcgcgcgaa cggcaccccc tgggccacgc actggtcgat
     1621 gatatcgacg ctgatctggg cgaggcggta cacgttcgcc tcgcgcgagc ggaagtcgcc
     1681 gcccttcacc gtgtcgtaga acagccgcca caccgaatcg ccgtcgttct ggtagttctt
     1741 ggcggcgttg atgccgccct gggcggcgat cgagtgcgcc cgccggggcg agtcctggaa
     1801 gcagaagcag gagacgccgt agcccagctc ggcgagcgag gccgcggccg acgcgcccgc
     1861 gagccccgag ccgaccacga tcacctcgaa ctttcgcttg ttcgccgggt tgacgagctt
     1921 catctcgaac cgatggcgct cccacttctg cgcgatcggc ccgtcgggga cgttggcacg
     1981 cagctccacg ctcatcgcac gaccccgctc aggacggcga gcggcaccag ggcgaatccc
     2041 cccgccacga ccagactgaa gatcagggcg aagccgcgcc gcgccgcgtt gtatttcgga
     2101 tggttgagcc ccagcgtctg gcaaccgctg tagatgccgt gatacatgtg caggcagagc
     2161 gtgcccatcg cgacgaggta gcccacggcg acgggcgccc gctgaaaggt ggcgatcacg
     2221 ttgtgatagg cgtcgccggg gatgaacgcg gggttcgccg ggccgaacgt cagatccagc
     2281 aggtggaacg tgacgaacgc caggatgatc accgcgcccc agcgcatggt gcgggcggca
     2341 taggtggtct cgacgaggcg cagcgcgtgg tagcccgcgg ggcgggccgc ccagctcgcc
     2401 cgcgtgacga cccaggcggc ccagatgtgg gcgcccaccg ccacgagcag gaagatccgc
     2461 acgatccaga gcagctgccc gcggccgaaa aacggcgcgc caacggtgcg cagtccttcg
     2521 gcgtaggcgt tgaaatgctc cggtccctgg aaaatcttga gattgccggc catgtgcccc
     2581 aggacgtagc ccaggaggac aatgcccgac acggccatga ccactttctt tccgatcagc
     2641 gagtgcgtga gggccgtcac gcgggacatc aatggctccc ttggcggggt ggaaggtcgc
     2701 acgcatgata cacagcgtgc tccaggcagt aaaggcgaag agccggagcg aaggctccgg
     2761 cgcttgtttc gcgcggcgaa acgccattac agggagcaac tactcgaatc aacgggatgt
     2821 tatcaaggtg acggccggcg gagtcgcact gacaagggct gctgcggcgg cgtcatggaa
     2881 ctccagtgag gtgatcacat gagcagcgaa tcgacgctga ccggtaccga acccaccgcc
     2941 aagacggctg acctggcggc ggtaacgcca gcgccctcgg cccgtccgcg cgtcgtcatt
     3001 gtcggcgcgg gattcggcgg gctgcgggcc gcgcgggcgc tgcggcgcac gccggtggat
     3061 gtgctgctcg tggatcgcaa caactaccac ttgtttcagc cgctcctcta tcaggtcgcg
     3121 acggcgggac tcgagccgga gcagatcgcc aagccggtgc gcgccatcct gcgcggccag
     3181 cgtaatttcg atttccgcct ggccgacgtg tcgggcgtgg acttcgccgg ccgccgactg
     3241 gtcaccggcg acggggcgat cgcgtacgac tacctgattc tcgccaccgg cgcccagacc
     3301 aatttcttcg gcctcacggg cgtggcccag cacgccctca cgctgaagga catccccgac
     3361 gcggtgcgca tccgcaacca cgtgctggcg tgcttcgaac gggcgatgtt cgagccggat
     3421 gcggagcggc gccgcgcgct gctgagcttc gtcgtcgtgg gcggcggccc cacgggggtc
     3481 gagatggcgg gatcgctgtc ggagctgatc cggctggtgc tcgtgaagga ctacccgcgc
     3541 ctcaatctca aggacgtccg gatcctgctg ctcgaggcgg gcgaccggct gctcgccgcc
     3601 ttcacgccgc gcctgtccga ggcggcggcc gcgacgctct ggaagaagca cgtcgaggtg
     3661 cggcacggcg ccatggtggt ggacttcgac ggcgccgccg cccggctgaa gagcggtgag
     3721 gtgatcccgg cgcgcaccct gatctgggcg gccggggccc gcgccgctcc gctcgccggg
     3781 cagctgggaa ttccgaccgc gcacctgggc cgcgtcgtgg tccagcccac gctgcagctc
     3841 gccggccacc ccgaggtgtt cgtgatcggc gattccgcct ttctcgaggc gcagggaccc
     3901 tcgctgccga tgatggcccc ggtggcgatc cagatgggcg agaccgcagc ggcgaacatc
     3961 cgccgggcgc tggcgggccg cacgccggag gatttccggt accgcgaccc cgggacgctg
     4021 gcgacgatcg gacgaaacgc ggccgtcgcg cgtatccatg gcgtgtcgtt caaaggattc
     4081 ccggcgtggg tggtctggct ggtggtgcac ctgatccagc tgatcgggtt tcgcaaccgc
     4141 ctgttcgtct tgaccaattg ggcgtgggat tacttctttt acgaaagagc ggtgcgactg
     4201 ataactgagg agcgacacta gaccagaaga cccaacactc gcacacacgt agccccggcc
     4261 agtggtcggg gctttgtgct gctgtggctc cgagtagagg acaatcaaca ggacagttcc
     4321 ggccggggcc gccgttcaga aatagaactg gcctgtgagc gctacatgcg cgagctccac
     4381 cgtgacgatg tccgtggtcc cggggctggg gttggggaag ggagctgaat tgctgttgtg
     4441 gctgtacgtc acatccacct gccactgagc tcccaggcct agatgcggtg tgacgagcca
     4501 cgtgcccccg aggttcacga aaacgccggc cccgtagcct ttgatcgttt gggtgacggt
     4561 gccctgtgta acactctgtc ggttgtagat gaacgacaaa cccatggttg tccagcgata
     4621 tacatggggg tcgatcaggt gataggcgcg ggtgccgagg ctgagagagg cactggcgct
     4681 gttgccgtgg ctcgccgggc tggcggtggt cgagccgtgg ctgtacccgc cgttgagatc
     4741 gaggagtatg gcgtgggttg ggctcgtgaa gtgtagggca ccagcgccag caaacccgct
     4801 cccaacgcgg aagtcgacgc cccactggcc cctatggaat agcgctgagt ccgggacctg
     4861 agccgctagg gcgggagtag ccaacaggaa ggaggcgaga aaggggaaac ggcgtggaca
     4921 catgggggcc ccctcgaaga gatggtttgg agcgacgtag tgctgctcag ggcaacttat
     4981 acgccgttat cgaatgacgt cctcatcaag ggacccgtga gcaggtgtcc ctggcgatac
     5041 acaggcgggc accgttggca gacagtaccc ttacttgcgg gtctgggctg agagctgctt
     5101 cttctatgac cgcgccgtcc gtttgatcac gcgttgagac ccggaggatc catcgtcatg
     5161 cgtcccccct cgtacgctgt cgtactcgct ctcactctgg cggcctgcgg caaggccgct
     5221 cccgcggcac agacacccgc gccggttccg ggcggcggag gggcggcgcc tccggcctcg
     5281 gcccggcagg atacgacgcg ggcgcggccg ggccaggccg cagagggtcc caagccctac
     5341 gcccaggtga tcaccaagga ggcgaccacc gattccggcg tgttcctggt gcaccgcatc
     5401 ggggagaagc tgttctacga aatcccgcgc ggccagctgg acaaggaatt cctgctggtg
     5461 atcgactacg ccgggactcc cgaggggacg cgctacggcg gcgaggagct cgacagccgc
     5521 gtggtgcgct gggagcggat ggccaatcgc gtgctgctgc gcggcaagag ctacgacatc
     5581 gtggcagaca gctccaccac cgtgtcgcgg gcggtgcggt tgtcgacgat cgagccggtg
     5641 ctgatgagct tcgacgtcgc cgcgttcagt cccaaggact cgaacctggt catcgaagtg
     5701 accaagctct acacgaccga cgtgcccgaa ttcaacacgc ggcgggtgtt ccggctgcgg
     5761 cgcctcgatc cggcgcgctc gctcgtcgaa tcggcgcggt cgttcccgac caacatcgaa
     5821 gtccgcgcgc tgcacacctt cgagggcgat tcggtgccgg gcaacgaccg cggactcggc
     5881 accgcgacct tcgagatgca ctactcgatg gtgctgctgc ccgagcgccc catggcgcgg
     5941 cgcctgtgcg acaatcgcgt cgggttcttc gcggtgcggc agatcgacta cggcctcgac
     6001 cggcccgtgg tcaccacgcg ctgctacatc acgcgctggc ggctcgagcc caaggatccc
     6061 ggcgccgcgg tgagcgatcc catcaagccg atcgtgttct acctcgaccc ggccaccccc
     6121 gagcggtggg cgccctggat catcaagggc gtgcaggcgt gggagccggt gttccgggcg
     6181 gccgggttct ccaacgccat cacggcgcgc cgcgcgccga cgccgcagga agacccggag
     6241 ttcgacctcg acgacgcgcg ctattcgacg atccgctggc tcccctcgca gatcgaaaac
     6301 gcctacggcc cgcacaccag cgacccgcgg acgggggaga tcctgcagtc gaacatcggg
     6361 tggttccaga acaccaccag cctgctgcag gcctggtact gggtgcaggc cggcgcggtc
     6421 gatccgcgcg cccgccagtt gccgctcccc gattcgatga tgggacagat ggtggcctac
     6481 gtcgcgaccc acgaggtcgg tcacaccctc ggcctgccgc acaacatgct gtcgtcgggc
     6541 ctctacccgg tggactcgct gcgcagtccc acttatacgc ggcgcaacgg cacctcgtat
     6601 tcgatcatgg attacgcccg caacaactac gtcgcccagc cgggagacga cgtccagctg
     6661 atgccgaccc tgggcccgtg ggactactac gtgatcgact ggggctaccg ccgcatgccg
     6721 ggcgccgcga cccccgacgt cgagcgaccc ctgctcgatt cgctcgcgcg gctgcaggac
     6781 gccagcccct ggctgcagtt cggcaacccg gacgggatcg atccccgcac ccagaccgag
     6841 gcgctgggcg acgacccggt gaaggccacg cagtacgggc tggcgaacat caagcggctc
     6901 gtcccgatgc tgctccccgc gacgaccacc gagacgctcg aggactattc gctgctcgac
     6961 gacctctacg accggctcat cgcgcaatgg ggtctcgaga tgaaccacgt cgccgtcgtc
     7021 ccggggggcg tgtggcggca cgagaagtat cccgatcagt ccggggtcat ccaccagccc
     7081 gtgccgcgcg cgcgccaggc ggaagcggtg cgcttcctca acgagaacgc cttcgccacg
     7141 ccgcgctact tcctcgacac cgcggtgctg cggcgcatcg agccgaccgg gttcgtggag
     7201 cggatccgca ccaggcagac ggcgctgctg aacacgctgc tccaggactc gcgcttgtcg
     7261 cggctggcgg agcagggcgc cacgatgccg gcgggaacgg cctacaccct caccgacctg
     7321 ctggacgatc tgcgcagcgg catcttctcc gaggtcggcg ccgcgcgacc cagcgtggac
     7381 gcctaccgcc gcaacctgca gcaggcgttc gtggatcaga tgaaccggct gatcaacacg
     7441 ccgctcgtga cgccgctacc gcccggcttc ttcggcttcc ccggcttcac ccccccgccg
     7501 ccccgtccgg ccgacgcgcg ctcggtggcg cgcctcgagc tgcgtgagct gcagacgacg
     7561 ctggggaccg cggcgggccg cgcggccgac cgcaccacgc gggcgcactt cctggatctg
     7621 caggcgcgga tcgggcagat attgaatccg ccgcggtgag ggctcgggag agcggctatc
     7681 ggctatcggc tatcggcgac accaggtcac gagcccgtgt ccgatagccg atagccgaca
     7741 gccgatagct tcctttcatg ctctccacct ccacctcgca ccgcgaatcc cgcttcacgc
     7801 gtcgccccct ggtgctggcg ctcaccgggt ggtacgccct cgtctgggtc gtcacggcga
     7861 tccggccgtt cgaccggcac gactggctac tcgagaacat cctggtcgtg ctcgtcctgg
     7921 cggtgctggt cgggtcctac cgggtgtttc cgctctccga tctgtcgtac ctgctgatcg
     7981 ccgcgttcat gacgctgcat gcgagcggcg cccactggac ctacgcccag gtgccgttcg
     8041 gcttctggct gcaacaggtg ctggggctcg cgcgcaaccc gttcgaccgg ctggtgcacc
     8101 tctgcttcgg gctgtggctc gcctatccca tccgtgaggt gttcctgcgg gtcgccaacg
     8161 cgcgcggcgt ctgggcctac tacctgccgc tcgacgtgac gctggcgttc agcgccctgt
     8221 acgagatcat ggagatggtg gtggccacgg tcgtggcccc gggcaccggg gacgcctggc
     8281 tcggcaccca gggcgacgtg tgggatgcgc agaaggacat ggcgatggcg gcgagcgggg
     8341 cgctgctctg catgctgatc accgccacgg tgcgccggct gcgccggcgg ccaccggtgc
     8401 tgctggcagg cgggtaatcg gcggtcatcg gtcgtcggta ccgatgaccg accaccgacg
     8461 atcgagaaca ctggcagcgg cgccaacccg accgcaactt actctcaccc aattccggag
     8521 ctccccgtgc gccccgtcca cgcactcatc gccggcctgg cgctcgcctt cgcctccgcg
     8581 ccgcttcacg ctcaacagat cgccacggtg gagctgtccc cagcgcacgc cagcgtcacc
     8641 gccggcgaca cgatccggtt ccgcgtcacg gcgcgagact ccgcgggggg cgtcgtgacg
     8701 ggggcgcaca ccgagtggtt cgcgaccccg ttcgacatcg ccgccgccga tgcgaacggg
     8761 ctcgtcacga cgacgcgcgc cgggcagacc tacgtcttcg ccctggtggg cgggaaaccg
     8821 gcattcgcga cgctcgacat cgtcgagcgc cccgcggcgc gactcgtgat cgccgcgccg
     8881 ggcggacgga ccagcctggt ggtgggagag gtcgtgccgc tcgtggcgca cgccgtcacc
     8941 gcggtgggcg atcccgtggg cgaggggcgc ggtcttcccg gcgtgcagtg gcgttcgctg
     9001 ggacccgcgg tcgccgcggt gtcgccgggc ggcgtcgtgc gggccagggc cgtcggtcgc
     9061 gctacgatcg cggcggcgct ggggtcggtc agcgcccgct gggaggtgac ggtgcggcgc
     9121 aatccggtca cctcgttgcg tatccgttcc gtggggccca cccgggtcgg ggatgtcgtg
     9181 gcgctgagcc cggacgcgcg cggggcgggc gggcggagcg agagcgacgt gccgctgcgc
     9241 tgggccgtga ccgggccggg cgccgcggtc tatccggacg ggcgcttcgt ggcgaccgag
     9301 ccggggatgt acaccgtgag cgccacggtg gggagccgcg tggcgacggt cgaagtaccg
     9361 gtgctgccgc gccacgatcc ccggggtgtc gagcgggtct cccacgtgat actgcccaag
     9421 gacgcccagg gcgccgagat ctggcccgtg ggggacgtcg cctacgtgtc caccatcggc
     9481 agcgcggtgt acgtcttcga catcgccgat ccggcggcgc ccaggctgac cgattccctg
     9541 gtggtggacg cccggctcct gaacgacctc atgacgaccg ccgacgggaa gatcctggtg
     9601 gtgagccggg aaggggccag cacccgcaag aacgggctgc tgttctacga ttgcgccgat
     9661 ccgcgccacc ccaagctcgt ctcggagttc accgaaacgg tctcgggtgg cgtgcacagc
     9721 gcctacgtct atcagcacta cgtcttcgcc accgacgacg cgacggggtc gctgcgcatc
     9781 atcgacttcg ccgatccgcg ggccccgaaa cagatcgcgc gctgggaggt gccgcgcgcc
     9841 attccggccc agccctatcc ggtcgaattc ctgaacatca tcccgc
//
LOCUS       DBNGZW010000680         2629 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_336083, whole genome shotgun sequence.
ACCESSION   DBNGZW010000680 DBNGZW010000000
VERSION     DBNGZW010000680.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_336083"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..648)
                     /locus_tag="AC16JC_16040"
     CDS             complement(43..648)
                     /locus_tag="AC16JC_16040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166261.1"
                     /translation="MTKQDVVALKARRSELSDQLESATSRRRSLAEQVRRADGVNKAG
                     LESRLTILDQRIVRIESELDQVGQQLASPQAAQFAAETRPPVNFGPQIAFSRIDPEPI
                     LICFTLFVLSPLALSISRLIWKRGSRSAMTVPALPAESGERLERIEQAMDAIAIEVER
                     VSEGQRFVTRLLSERGGAALHAVPPMGEPLRAPAGEASRVR"
     gene            complement(852..1760)
                     /locus_tag="AC16JC_16045"
     CDS             complement(852..1760)
                     /locus_tag="AC16JC_16045"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR5166262.1"
                     /translation="MTGTSRCSRFRSPRALLRRTALLAAVSVALGSVQPAHASAQEPA
                     PGDDRKVVPRDTAHAERHQTLFTYRDAILAGGFAGLTVAMFPLDKNIARQIRQDSTFN
                     GFTQKSAVGFENISSPGAYVIGGGLYLYGRLAKKPNLTDLAWHGTEAVLVANVVTGFM
                     KGTLGRARPFVSNDSNPRDFRFGGGFSSSDRQSFPSGHTTTAFAAAAAVTSEIRRLHP
                     KALPYVATVMYGGATLVGLSRMYHNKHWASDVALGAAVGTFSGLKVVRYSHAHPDNKL
                     DRVILKTVIAPDGHGGGYLAFSLPAP"
     gene            complement(1868..2140)
                     /locus_tag="AC16JC_16050"
     CDS             complement(1868..2140)
                     /locus_tag="AC16JC_16050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166263.1"
                     /translation="MPLCPICGGAMWDDRESKRNPRAPDFKCRNKPRERGGPGCEGVI
                     WPARDGSPSPYPPPMPRRAGSPSSRPPSEAPPAWDGPPLDDDDIPF"
BASE COUNT          399 a          894 c          956 g          380 t
ORIGIN      
        1 agaaaactag gcagcggagc gcgccggccg cgcgatcgcg gctcagcgca cgcggctcgc
       61 ctcgccggcg ggcgcacgca gcggctcgcc catgggcggc acggcgtgca gtgccgcgcc
      121 accccgctcc gacagcaacc gcgtcacgaa gcgctgaccc tcggagaccc gttccacctc
      181 gatcgcgatc gcatccatcg cctgctcgat tcgctcgagg cgctcacccg attcggccgg
      241 caatgcgggc accgtcatcg ccgaacgcga tccgcgcttc cagatgagcc gcgagatgga
      301 cagtgcgagg ggtgagagca cgaacagggt gaagcagatg aggatcggct cgggatcgat
      361 tctggagaac gcgatctggg gtccgaagtt caccggaggc cgcgtttccg cggcgaactg
      421 cgccgcctgc ggcgaggcga gctgctgacc gacctggtcg agctccgact cgatgcgcac
      481 gatgcgctga tcgaggatgg tgaggcgcga ctcgagcccc gccttgttga cgccgtcggc
      541 acggcgcacc tgctcggcga gcgaacggcg ccggctggtt gcgctctcga gctgatcact
      601 gagttccgag cggcgcgcct tgagggcgac gacgtcctgc ttcgtcatcg cgcctcccgc
      661 aagggagccg gcgtagacca cgttcgaagc gggcgcggac ggagtcgcgg gcgcgggagg
      721 cggcggcggt ggtgcggacg gctgctggaa gggtgacggc atgggctgcc ctacggcgag
      781 gacgaggtcc aggttgcacc gggagcgtgc atctggcgtg accggcctcg tcgagcgcgg
      841 gcgcgcctag ctcaaggtgc gggcagcgag aaggcgagat aacccccgcc gtggccgtcc
      901 ggcgcgatca cggtcttgag gatgacgcga tcgagcttgt tgtccggatg cgcatgggag
      961 tagcgcacca ccttgagacc gctgaaggta ccgaccgccg cgccgagcgc cacgtcgctc
     1021 gcccagtgct tgttgtggta catccgcgag aggccgacca gagtggcccc accgtacatc
     1081 accgtcgcga cgtacgggag cgccttcgga tgaagccggc ggatctcgct ggtcaccgcc
     1141 gccgccgccg cgaacgccgt cgtcgtgtgc ccggagggga acgactgccg atccgacgag
     1201 gagaacccgc cgccgaagcg gaaatcgcgc ggattgctgt cgttcgacac gaagggacgc
     1261 gcacggccga gcgtcccctt catgaatccc gtcacgacgt tggccacgag cacggcctcc
     1321 gtgccgtgcc aggccaagtc cgtgaggttc ggcttcttgg cgaggcgacc gtagaggtag
     1381 aggcccccgc cgatgacgta cgcgccgggc gagctgatgt tctcgaagcc gacggccgat
     1441 ttctgcgtga agccgttgaa ggtcgagtcc tggcggatct ggcgggcgat gttcttgtcg
     1501 agcgggaaca tcgccacggt gagcccggcg aagccgccgg ccaggatcgc gtcgcgatag
     1561 gtgaacagcg tctggtggcg ctcggcgtgg gcagtatcgc gcgggacgac cttgcggtcg
     1621 tcgccggggg caggctcctg cgccgatgcg tgcgcgggtt gtaccgagcc cagcgcgacc
     1681 gaaacggcgg ccagcaacgc cgtgcgccgg aggagagcgc gtggcgacct gaatcgagaa
     1741 cagcgcgaag tgccggtcat gcccgccgga aatgcaggaa tcgggcgcga gtgcgcgggt
     1801 gtgcgggtgc gcggggcggg tgagagttca ccgactcacc gactcaccga ctcaccgact
     1861 caccgcccta gaacggaatg tcgtcgtcgt ccagcggcgg gccgtcccag gcggggggcg
     1921 cctcggacgg cggccgcgag gagggcgagc cggctcggcg cggcatcggc ggggggtacg
     1981 gactcggcga gccgtcccgc gcgggccaga tcaccccctc acacccgggg ccgccgcgct
     2041 cgcgcggctt gttccggcac ttgaagtcgg gtgctcgcgg gttccgcttc gactcccgat
     2101 cgtcccacat cgcgccgccg cagatcgggc agaggggcat cccgttcgcg tccacgcgcg
     2161 ggccggccgc aggtgcgcca cggccggcgg atgccgtcgg ggcctctgcg ttctttgtag
     2221 tggaggcagg tggcgtgcgc ggcgcggcgg gctggctcgc gcccgacgta gcgctccgcg
     2281 gcggccagat caccccgtcg cagcttcggt ccttgcactt gaagtcgggc gccttgggat
     2341 tgcgcttgcc ttcgcggttg tcccacatcg cgcccccaca cttcgggcag ctgggagcgg
     2401 cggtcggggc gtcgggtgag ttcggctcgg acatacggcg acgtgaagga aggagtgctc
     2461 caaactagtc gctcgatccg tccacgtcgc caccaggact gtgccggccg gatcgcgccg
     2521 cggcagcgac gacacggaga cgcctcaccg cttcaccgct ccacccactc accgctccac
     2581 ccactcaccg ctccacccac tcaccgcttc atcagtcccc ggccgactt
//
LOCUS       DBNGZW010000681         5464 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1070142, whole genome shotgun sequence.
ACCESSION   DBNGZW010000681 DBNGZW010000000
VERSION     DBNGZW010000681.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5464)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5464)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5464
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1070142"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3152
                     /locus_tag="AC16JC_16055"
     CDS             <1..3152
                     /locus_tag="AC16JC_16055"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HIR5166264.1"
                     /translation="SDGRYTISVPAARVRGQEVILRVRAIGFKPASKSLTLVAGAQTV
                     DFTLVYDVNLLQAVVVTGVQEATEAVKVPFFVSRVDSSQMPVVGQDPLTQLKGKVPGV
                     EISSTSGRPGAQPAVLLRGPTSINSQGRNQDPLYIVDGIQITGDLPDLNPSDIESVEV
                     VEGAAGSALYGAYAGNGVINITTRSGRRALDGVHFSLRSEGGVGDIERDFGLARFHAL
                     VTDETGTRFCANVTGQPLCARTFDYRAEQARINNDPGAFALNPVGFPVDPGATIAGNV
                     LRQRFQIQPWPGTSYNAVAQTVHPNTYLRNNGDMTGRFGGTTFYASASNETDGGAIRF
                     LQGFRRYSFRANVDQVIGTDWTVAVRSFYSRSTQDGLNQEGGGQAFFRLTRVPGIANV
                     LQKDTLGRLYIRPNLQGGGSQNENPLWSLQNTERSDVSDRFIGGGTVQFTPATWVKFE
                     GSLSYDLRARNSLQFNDKGFRTTTNNPGTNNGSIFRQNAGDRALNAAVSASFNHDFAA
                     DLRTHYLLSYSYRYAASDFENGQGNTLLAKGITSLINATANQAIRSSLTELRGIGLIA
                     GGGLEYKNRYVLDGLIRRDGLSVFGSDQRWATFGRVAGTWLVAREPWWRIPQISELKL
                     RGSYGTAGGAPNLTAQYETFTIGAGGSFTLDLLGNPDLRPELHRDVEAAADVELLSRY
                     GLNVTYAHSTIDHQILPVPVSTSTGFQTQWRNAGTLVNKTWEASLNVPLIQRRDVSWS
                     MRFIYDRNRSVITALDVPPYFYGATLQATDQIFQAKVGERLGTFYGRKFITSCSELPA
                     PYTGDCGGPASSFQRNDQGFIIWVGAGNNPGMGITNNLWEAQLPASSAPWGVAVNWGM
                     PIIMRGAGATGQNATIVPLGNALPNFRFSVSQDFSWQRLSVYALLDAAIGQHVWNQGY
                     HWAHLDFLSKDVDQNGKSVQSAKPISYYYRAPAPDGTGLGGLYDILGPNNFSVEDASY
                     AKLREVLVSYRIGPLFGRGDWTASLVGRNLLTITGYRGFDPEVGIAGGQVNSSAINAI
                     DAFTFPNVRTFTFGLSTRF"
     gene            3217..4905
                     /locus_tag="AC16JC_16060"
     CDS             3217..4905
                     /locus_tag="AC16JC_16060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166265.1"
                     /translation="MRTARSVLLGAALLGVLACTDFVQVDNQNNPDRNKLLATAADLE
                     NFIAASYNMVHQGTIGGATVLSGGGNDALQPQLFTMGLENVSGLANFAMGPRGAIPRN
                     PIDNTRGSQGNIGNFRDYAVEHRAARMATIGLAKLESSTLGSTGRDNRAKAFALFSQG
                     VAEGNLALAYDSASILSPADGPLDIIPLSGYNTVMQAALKLLDSAIAVATADPSGFPL
                     PSTWINGNALSAAQFIALAHSYKTLFRANVARNPAERAAVDWNAVLADASAGITADLT
                     ISMQPPPTGAWDVVWVVQAFATGSANWHQMSQFILGMADTSGGYDVWLATPRASRVAF
                     LVVTPDLRMPQGTTRAAQVGDTLRTGFRTFAGLPYIRNRPPGDDQPGDPFGISMYDFF
                     RSRAFFSATRIGPYPVMTAASIRLLAAEAQLRLGNVPAAAALIDVSRVGKGGLPSLVT
                     AGIADTLTAVPGGGGCVPRVPNPATGFKSAKCGTIWDALKWDYRIETAYTGYGNWFFP
                     SRGWGDLPEGTTVNWPVPYQEMDARGQSFYGLGGVGLPGGAVPGNYGLFAGGVY"
     gene            4910..5365
                     /locus_tag="AC16JC_16065"
     CDS             4910..5365
                     /locus_tag="AC16JC_16065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166266.1"
                     /translation="MRRGSLLWLGILAGGGCYSYSPLPTLTPAPGSRVALVLSDQGRV
                     GMASSIGPGVARVEGSLLEASDSAYRLQVSDVYGIDGKHSQWTAETVLVRRDYVANTL
                     ERHFSRPRTYLAVAGASAAFVSFVLTRGLLGLGGSGPGSGPGDGGGNNQ"
BASE COUNT          889 a         1831 c         1819 g          925 t
ORIGIN      
        1 cgagcgatgg ccgctacacg ataagcgtgc cggccgcccg tgtacgcggt caggaggtca
       61 tcctgcgtgt gcgggcgatc ggtttcaagc ccgcgagtaa gtcgctgacg ttggtggccg
      121 gggcgcagac ggtcgacttc acgctggtct acgacgtgaa cctgctccag gcggtcgtgg
      181 tgaccggggt gcaggaggcg accgaggcgg tgaaggtgcc gttcttcgtg tcgcgcgtcg
      241 attcctcaca gatgcctgtc gtcggtcagg acccgcttac ccagctcaag ggcaaagtgc
      301 ccggcgtgga gatttcctcc accagcgggc gtcccggcgc acagcccgcg gtgctgctgc
      361 ggggcccgac ttcgatcaac tcccagggac gcaatcagga tccgctctac atcgtggacg
      421 ggattcagat cacgggtgac ctgcccgacc tcaatccatc cgacatcgag agcgtcgagg
      481 tggtggaagg cgcggcgggc tcggcgctgt acggcgccta tgccgggaac ggcgtgatca
      541 acatcaccac ccgctccggc cgccgcgctc tggacggcgt gcatttcagc ctgcggtccg
      601 agggtggcgt gggggacatc gagcgcgact tcggtttggc gcgtttccat gcgctggtca
      661 ccgacgagac cggcacccgg ttctgcgcga acgtgacggg acagccgctg tgcgcgcgga
      721 ctttcgacta ccgggccgag caggctcgca tcaacaacga cccgggggcc ttcgccctga
      781 atccggtcgg gttcccggtg gatccgggtg cgacgatcgc cggcaacgtg ttgcggcagc
      841 ggttccagat ccagccgtgg cccggcacgt cctacaacgc cgtggcgcag acggtgcatc
      901 ccaacacgta cctgcgcaac aacggcgaca tgaccggccg gttcggcggc acgacattct
      961 acgcgagcgc ctcgaacgag actgacggcg gggcgatccg cttccttcag gggttccggc
     1021 gctattcgtt ccgggccaac gtggaccagg tgatcggcac ggattggacg gtggcggtgc
     1081 ggagcttcta cagccgcagc acacaggatg ggctgaacca ggaaggcggc ggccaggcct
     1141 tcttccgtct gacgcgcgtg ccgggaatcg cgaatgtgtt gcagaaggat accctgggcc
     1201 gactctacat ccggcccaac ctccagggcg gcggctcgca gaacgagaat ccgctctggt
     1261 ccttgcagaa caccgagcgc tcggacgtga gcgaccggtt catcggcggc ggaacggtgc
     1321 agttcacgcc ggccacatgg gtcaagttcg agggcagtct gagttacgac ctgcgcgctc
     1381 ggaactcgct gcagttcaac gacaagggct tccggacgac gacgaacaac ccgggcacaa
     1441 acaatggctc gatcttccgc cagaacgccg gtgaccgggc cctcaacgcg gccgtgagcg
     1501 cgtcgttcaa tcacgatttc gcggcggacc tgcggaccca ctacctgctc agctactcct
     1561 accgttacgc cgcgagcgac ttcgagaacg gacaagggaa tacgctgctc gccaagggga
     1621 ttacgtcgct gatcaacgcg accgccaacc aggcgatccg ctcctcgctc accgagttgc
     1681 gcggcatcgg gctgatcgcg ggcggcgggc tggaatacaa gaaccgctac gtactcgatg
     1741 ggctgatccg gcgcgacggt ctttccgtgt tcgggagcga tcagcgctgg gccaccttcg
     1801 gccgggtggc gggcacctgg ctggtcgcgc gggagccttg gtggcggatc ccgcagatca
     1861 gcgagctgaa actgcggggc tcctacggga ctgccggcgg cgcgccgaat ctcaccgcgc
     1921 agtacgagac cttcaccatc ggggcgggcg gctcgttcac gctcgacctg ctcggcaacc
     1981 cggatttgcg tcccgagctg catcgcgacg tggaagcggc agccgacgtc gagctgctga
     2041 gccgctacgg cctcaacgtg acgtacgcgc attcgacgat cgaccaccag attctgccgg
     2101 ttccggtctc cacgtcgacc ggcttccaga cgcaatggcg caatgccggg acgctggtca
     2161 acaagacctg ggaggcctcg ctcaatgtgc cattgatcca gcgccgggac gtttcctggt
     2221 ccatgcggtt catctatgac cgcaatcgct ccgtgatcac cgccttggac gtgccgccct
     2281 acttctacgg tgcgacgctt caggctaccg accagatctt ccaggccaag gtgggtgagc
     2341 gcctcggcac gttctacggc cgcaagttca tcacgagctg cagcgagctg ccggcgccct
     2401 acaccggcga ttgcggcggc ccggccagct cgttccagcg caacgaccag ggcttcatca
     2461 tctgggtcgg cgccggcaac aaccccggca tgggcatcac gaacaacctc tgggaagccc
     2521 agctcccagc gtccagcgcc ccgtggggcg tcgccgtcaa ctgggggatg cccatcatca
     2581 tgcgcggggc gggcgcgaca ggtcagaatg cgacgatcgt cccgctgggt aacgcgctcc
     2641 ccaattttcg cttctcggtt tcgcaggatt tctcgtggca gcgtctgtcg gtgtacgccc
     2701 tgctcgatgc agcgattggg cagcatgtgt ggaaccaggg gtaccactgg gcccacctcg
     2761 acttcctatc caaggatgtc gaccagaacg ggaagtccgt ccagagcgcg aagccgatca
     2821 gctactacta ccgggcgccc gcgcctgacg gaaccggcct gggcgggctc tacgacatcc
     2881 tgggacccaa caacttctcg gtggaggacg cgagctacgc taagctgcgc gaggtgctgg
     2941 tctcctaccg catcgggcca ctgttcggcc gtggcgattg gacggcgagc ctcgtcggtc
     3001 gcaacctgct gacgatcacc ggctatcgcg ggttcgaccc cgaggtgggt atcgcgggcg
     3061 ggcaggtgaa ctcgtcggcg atcaacgcca tcgacgcgtt cacgttcccg aacgtgcgga
     3121 cgttcacctt cggcctgtcc accaggttct agaccagggg cgccgccggc ggggttgtcc
     3181 cgttcggcgg cggatccctc cgctccggag aaaccgatgc gaactgctcg ctctgtactg
     3241 ctcggcgccg cgctgctcgg ggtcctcgcc tgcaccgatt tcgtgcaggt ggacaaccag
     3301 aacaaccccg accgcaacaa actcctggcc acggcggccg acctcgaaaa cttcatcgcc
     3361 gcctcctaca acatggtcca ccagggcacc atcggcggcg ccaccgtcct gagcggcggg
     3421 ggcaacgacg ccctgcagcc acaactcttc accatgggat tggagaacgt ctcgggcctc
     3481 gccaacttcg cgatgggtcc ccggggagcg attccgcgca acccgatcga caacacccgg
     3541 ggcagtcagg gcaacatcgg caacttccga gactacgccg tcgagcatcg cgcggcgcgg
     3601 atggccacca tcgggctggc gaagctggaa tccagcacgc tcggctcgac gggccgcgac
     3661 aaccgggcga aggcgttcgc gctcttctca cagggtgtgg cggaagggaa tctcgccctc
     3721 gcctacgatt ccgcttccat cctgtcgccc gccgacggcc cgttggacat catccccctc
     3781 tccggataca acacggtgat gcaggccgcc ctgaagctgc tcgattcggc gatcgcagtc
     3841 gcgacggcgg atccgagcgg tttcccgctg cccagtacct ggatcaacgg caacgcactc
     3901 agcgccgcgc agttcatcgc gctggcgcac tcgtacaaga cgctgttccg cgccaacgtg
     3961 gcccggaacc ccgccgagcg ggccgccgtc gattggaacg cggtgctggc cgatgccagc
     4021 gcggggatca ccgcggacct caccatcagc atgcagccac cgccgacggg ggcctgggac
     4081 gtggtctggg tcgttcaagc cttcgctacc gggtcggcca actggcatca gatgtcgcag
     4141 ttcatcctgg ggatggcgga tacgtcgggg ggttacgacg tctggctcgc gacgccgcgc
     4201 gcgagccggg tggcgtttct ggtcgtgact cccgacctgc ggatgcctca gggcacgacc
     4261 cgtgccgcgc aggtgggaga tacgttgcgg accggcttcc gcaccttcgc cgggttgccc
     4321 tacatccgga atcgtccgcc cggcgacgac cagcccggcg atccgttcgg gatctcgatg
     4381 tacgacttct tccggtcgcg cgcgttcttc agcgccaccc ggattggtcc ctatcccgtg
     4441 atgaccgcgg cgtcgatccg gctgctggcg gcggaagcgc agctgcggct ggggaacgtg
     4501 cctgcggcgg cggctctgat cgatgtgtcg cgcgtgggca agggcggcct gccgtcgctg
     4561 gtgaccgccg gcatcgcgga cacccttacg gcggtccccg gcggcggagg ctgcgtgccc
     4621 agggtcccca accccgcgac gggattcaag tccgccaagt gcgggacgat ctgggatgcg
     4681 ctgaaatggg actaccggat cgagaccgcc tacaccggct acggcaactg gttcttcccg
     4741 tcccgcggat ggggagattt gccggaaggc accacggtca actggccggt gccttatcag
     4801 gaaatggacg cgcggggcca atcgttctac ggcctgggcg gagtggggct gcccgggggc
     4861 gcagtcccgg gcaactacgg gttgttcgcc ggtggggtct actgatacga tgcggcgcgg
     4921 cagcctgctg tggctcggaa ttctggcggg tggggggtgc tactcctatt cccccctgcc
     4981 caccctcact cctgctccag gcagccgggt ggcgctggtg ctttccgacc agggccgcgt
     5041 cgggatggcc tcgagcatcg gtcccggagt cgcccgcgtg gaagggtcgc tgctcgaggc
     5101 ttccgactcc gcctaccggc tgcaggtgtc ggacgtatat ggcatcgatg gcaagcacag
     5161 ccaatggacc gccgagaccg tcctggtgcg gcgcgactat gtggcgaaca ccttggagcg
     5221 ccacttctcc cggccccgga cctacctggc ggtggccggc gcatcggcgg ccttcgtctc
     5281 gttcgtgctg acgcgcggtc tactgggcct cggtggatcg gggcccggca gcgggccggg
     5341 cgatggtgga ggaaacaacc aatgagccgt caacgcaacc tttctccagt cgagcgtgct
     5401 atgcatccac tccggttcta tgcgctgtcg ggcgtcgcgt tcctcgtcgc ggggtgcact
     5461 aagg
//
LOCUS       DBNGZW010000682         5856 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_405734, whole genome shotgun sequence.
ACCESSION   DBNGZW010000682 DBNGZW010000000
VERSION     DBNGZW010000682.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5856)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5856)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5856
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_405734"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="AC16JC_16070"
     CDS             <1..447
                     /locus_tag="AC16JC_16070"
                     /inference="COORDINATES: protein motif:HMM:NF039902.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HIR5166267.1"
                     /translation="PEDLLPVVAPQLQTLGLDGDRAAVLRAIGAVKTRSRTTLDVAKQ
                     VAVRLDARHVTLDEKAQKEIAKDPEGYTSALQAAIAALRDVDWSPEALEKALRALADA
                     RGVPAGKVFQPIRIALTGGTVSEPVNELLFVIGKDAALRRLAAAVR"
     gene            complement(457..1230)
                     /locus_tag="AC16JC_16075"
     CDS             complement(457..1230)
                     /locus_tag="AC16JC_16075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166268.1"
                     /translation="MLLHVVGLGVAIALKIEFKRSPVRYVQIAPEPELPPFGGVRPGP
                     SSGNRLGRGKVRQAPPVVAVEQPPPDTAQSSPQALPAPAPVAARPGLTFSPGAGDGRL
                     WVSPRPALPEDIADAIYEDRAPRDSVIVRRLRAMVDSLNVIIDSEQREHRAPSWVARG
                     ADGKPVWGLDPGGLYVAGVKVPTPVLALLGSLLPPGNYDEAVRQRHLADMRADIMQAA
                     ARTENLEMFRRYVRELRARKQAERDAERRQRGDTTKATP"
     gene            complement(1283..2452)
                     /locus_tag="AC16JC_16080"
     CDS             complement(1283..2452)
                     /locus_tag="AC16JC_16080"
                     /inference="COORDINATES: protein motif:HMM:NF012382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5166269.1"
                     /translation="MPKLSNRLAQLPGYPMAEIPAIKRRLLQQGVDLIDVGAGDADFA
                     PPDIAVDALSRAAREPAMSRYAFQIGLPAFREAAAAYLQRRFKLTVDPFTELHPLLGS
                     KEGIANLSFSVLDPGDVAIVPEPGYAVYHGATVLAGATPHLVPLTPRTDFLLEMEELP
                     ADVVRRAKLVYLNYPNNPTAAVAPREYLERTVAFCLRHDILIVYDNPYCEITLDGYVA
                     PSIFEIDGAREVAVEFHSLSQSFCMTGWRLGWAVGRSELIAALARVKNYIDTGAFLAI
                     QAAGAAVLERAAELSRGYAERFRERRDAMVAALRAEGFEVESPRATMYLWIPLPDGMP
                     SAAFQRRALEDVGVVTLPGTGFGAGAEGFFRIALTVATPRLVEAAQRLGKVLAAV"
     gene            complement(2499..3032)
                     /locus_tag="AC16JC_16085"
     CDS             complement(2499..3032)
                     /locus_tag="AC16JC_16085"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR5166270.1"
                     /translation="MSVARSWAGVAGITVLALGGCAPTPGQGPPPGRSEPSGRRFVNP
                     GTLAALDGFTHAVRLGFVTYVSGEVPLDSSGTLVGPGDLAAQAKQAFANLALTLRIAG
                     NEPQDIVKLTVYVVNYHPQDFALIRHAAPDFFPARNPPAGIVVGVAALPRDGMLIAVD
                     AIAQAPALFRPLSERRE"
     gene            complement(3075..5612)
                     /locus_tag="AC16JC_16090"
     CDS             complement(3075..5612)
                     /locus_tag="AC16JC_16090"
                     /inference="COORDINATES: protein motif:HMM:NF024703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HIR5166271.1"
                     /translation="MSVERLAPEVQDLLRAEIVAARGREVSFVARADGRGAIAEARVV
                     ARGTVDAVLALPGSARRGEMLLHNHPSGDLEPSGADLSVAARLHDDGVGFGIVNNEVT
                     ELYVVVECPRAKSLEPLDPVTTARLLGIDGPVADAVGREGFEDRPAQRDMAAYIADAY
                     NDGGVALLEAGTGVGKSFAYLVPALLWGQANAERTVVSTNTINLQEQLVGKDLPVLAR
                     ALATPDHEPTFALLKGWRNYLCLARLEQARGAQETLFETNRSTELEGIAAWAARSADG
                     SLADLPDDPSEDVWDAVAAESDLCTRLKCPHFERCFVFQARRRAAEADVVVVNHHLLA
                     SDLAVRLAADNWMEAAVLPPYRRLILDEAHHLEDVAAAHLGAQVSQMGVQRLLGRLER
                     GGRGLMPALHAELTARDDLLSAASRDLLEQRLLDALRAARRWSEELFTLLGRRLEREP
                     AGQSGPMLRLTEAFTEDAVWAEGLEVALDNLLAAFAGLRDGVETIADRLSLQDGADRP
                     AALLGELRGVVRRLDLAARGLTASLRPGRDAAAEGAPAVRWLERRGRKGTNLSLAAVP
                     LDLASVLKDALFDRVETVVLTSATLASGNDFGFLEDRLGLSLPPSRVVTREILPSPFD
                     FVSQCLFGIPTDVPEPRDDAEGHDAAVARVLVELAHAADGGVFCLFTSHRALRRTADA
                     VRGRIGGRWPLLVQGEDQRDRLLRRFRESGSAVLLGTDSFWEGVDVPGRALRVLILSK
                     LPFKVPSEPLTAARVERLEQQGLDGFSNYLVPLAGLKLKQGFGRLIRSRSDVGAVVLL
                     DRRAVTKRYGVRILEGLPRATRVVASWEDIRDACEEFFARHATALRG"
     gene            complement(5609..>5856)
                     /locus_tag="AC16JC_16095"
     CDS             complement(5609..>5856)
                     /locus_tag="AC16JC_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HIR5166272.1"
                     /translation="ERLKPGVTILADEEGAPWVKYIHGDLGKGTWTFFGGHDPEDPQH
                     QIGDPPTDLSLHTHSPGYRLILNNVLFPAAKKRELKT"
BASE COUNT          860 a         2167 c         2036 g          793 t
ORIGIN      
        1 cccgaggacc tgctccccgt cgtggccccg cagctccaga cgctgggcct ggacggcgat
       61 cgcgccgccg tgctgcgcgc gatcggcgcc gtgaagacgc ggagccgcac cacgctcgac
      121 gtcgccaagc aggtcgccgt gcggctcgac gcgcggcacg tgacgctcga cgaaaaggcc
      181 cagaaggaga tcgccaagga cccggagggc tacacgagcg cgctccaggc ggcgattgcc
      241 gcgctgcgcg acgtggactg gtcgccggag gcattggaga aagcgctgcg ggcgctcgcc
      301 gacgcgcgcg gggtgccggc cggcaaggtc ttccagccga tccgcatcgc cctgaccggc
      361 ggcacggtgt cggagcccgt gaacgagctg ctattcgtga tcggcaaaga cgcggcgctg
      421 cggcggctcg cagcggcggt gcgctgagtc cgagagctac ggcgtcgcct tcgtggtgtc
      481 gccacgctgc cgccgctcgg cgtcgcgctc cgcctgcttg cgcgcccgca gctcccgcac
      541 gtagcgccgg aacatctcga ggttctcggt ccgggccgcc gcctgcatga tgtcggcgcg
      601 catgtcggcg aggtgacgct ggcgcaccgc ctcgtcgtag ttccccggcg gcaggaggct
      661 ccccagcagc gccagcaccg gcgtcgggac cttgaccccc gccacataca ggccgccggg
      721 atcgagtccc cacaccggtt tgccgtccgc cccgcgtgcc acccacgacg gtgcccggtg
      781 ctcgcgctgc tccgaatcga tgatgacgtt cagggagtcc accatcgcgc gcagccggcg
      841 cacgatcacc gagtcgcgcg gtgcgcgatc ctcgtagatc gcgtcggcga tgtcctcggg
      901 aagcgcggga cgcggactca cccacagcct gccgtcgccc gcaccgggcg agaaggtcag
      961 gccgggtcgc gctgccaccg gcgccggtgc cgggagggcc tgcggtgagc tctgcgcggt
     1021 atcgggggga ggctgctcta ccgcgacgac gggcggggcc tgcctgacct ttccccgccc
     1081 cagccggttc ccgctgctcg ggccggggcg cacgccgccg aacggcggca gctccggctc
     1141 gggggcgatc tgcacgtacc gcaccggcga gcgcttgaac tcgatcttca gcgcgatggc
     1201 gacaccgagc cccacgacgt gcagcagcac ggcgaccacc cacccgatgg cgggacgggg
     1261 cccggtccgg gccggcgcga actcagacag cggccaacac cttgccgaga cgctgggcgg
     1321 cctcgaccag ccgcggcgtc gcgacggtga gcgcgatccg gaagaagccc tcggctcccg
     1381 cgccgaagcc cgtgcccggg agcgtcacca cgccgacgtc ctcgagcgcg cgccgctgga
     1441 acgccgccga cggcatgccg tcggggagcg gaatccacag gtacatcgtg gcccgcggcg
     1501 attccacctc gaacccctcg gcacgcagcg ccgcgaccat cgcgtcgcgc cgctcgcgga
     1561 accgctcggc gtagccgcgg gacaactccg ccgcgcgctc cagcaccgcg gcgcccgcgg
     1621 cctggatcgc caggaacgcc ccggtgtcga tgtagttctt cacgcgcgcc agcgcggcga
     1681 tcagctcgga ccgccccacc gcccagccga gccgccatcc cgtcatgcag aagctctgcg
     1741 agagcgaatg aaattcgacc gcgacctccc gcgccccgtc gatctcgaag atgctgggcg
     1801 cgacgtagcc gtcgagggtg atctcgcagt agggattgtc gtagacgatg aggatgtcgt
     1861 gccggaggca gaacgcgacc gtccgctcga ggtactcgcg tggggcgacg gccgcggtcg
     1921 ggttgttggg atagttcagg tagacgagct tggcgcgccg caccacgtcg gccggcagct
     1981 cctccatctc gagcaggaag tcggtgcgcg gggtgagcgg gacgaggtgc ggcgtggcgc
     2041 ccgccagcac cgtcgctccg tggtacacgg catagcccgg ctcgggcacg atcgcgacgt
     2101 cccccgggtc gaggacgctg aaggagaggt tcgcgatccc ctctttcgac cccaggagcg
     2161 gatggagctc ggtgaacgga tcgacggtca gcttgaagcg ccgctgcagg tacgccgccg
     2221 cagcctcgcg aaacgccggc agtccgatct gaaacgcata ccggctcatg gccggctcgc
     2281 gcgcggcgcg cgacagcgcg tccaccgcga tgtcgggcgg cgcgaagtcg gcgtcccccg
     2341 ccccaacgtc gatcagatcg accccctgct gcaacaggcg ccgcttgatg gccggaatct
     2401 cggccatcgg atagccggga agctgcgcga ggcggttgct gagcttgggc actgtcacct
     2461 caggttatgg aggtcggtgg aagacaccgc cctccaattt actctctccg ctcgctcagc
     2521 ggccggaaca gcgccggcgc ctgggcgatc gcatccaccg cgatgagcat accgtcccgt
     2581 ggcagcgccg ccacaccgac cacgatgccc gccggcgggt tcctggccgg gaagaaatca
     2641 ggggcggcgt ggcggatcaa cgcgaagtcc tgtgggtggt agttcaccac gtacaccgtg
     2701 agtttcacga tatcctgcgg ctcgtttccc gctatccgca gcgtcagtgc cagattggcg
     2761 aacgcctgct tcgcctgcgc ggcgaggtcg cccggtccca ccagcgtgcc gctgctgtcg
     2821 agcgggacct cacccgatac gtaggtgacg aacccgagcc ggacggcgtg cgtgaagccg
     2881 tcgagcgcgg cgagcgtccc ggggttcacg aaccgccgcc ctgacggctc cgaccggccc
     2941 ggcggagggc cctgcccggg cgtcggcgcg cagccgccca gggccaacac cgtgattccc
     3001 gcgaccccgg cccacgatcg tgccacgctc atcccctggc ctcctgacag tagtgttcgt
     3061 tgatggagtt acgctcagcc ccgcaatgct gttgcgtggc gtgcaaagaa ctcctcgcag
     3121 gcgtcccgga tgtcctccca gctggccacc acccgggtgg cccgcggcag tccttccagg
     3181 atgcgtaccc cgtagcgctt ggtcaccgcg cgccggtcca acagcacgac ggcgccgacg
     3241 tcggagcggc tgcggatcag gcggccgaac ccctgtttga gcttgagacc ggccaggggc
     3301 acgaggtagt tgctgaaccc gtccaacccc tgctgctcga gccgctccac tcgcgccgcc
     3361 gtcagcggct ccgacggaac cttgaacggc agttttgaca gaatcaggac gcgcagcgcg
     3421 cgccccggga catccacgcc ttcccagaac gaatcggtgc cgagcagcac ggcgctcccg
     3481 gactcgcgga accggcgcag cagccggtcg cgctggtcct cgccctggac gagcagcggc
     3541 caccgcccgc cgatccgccc gcgcacggcg tccgccgtcc ggcgcagcgc gcggtggctc
     3601 gtgaacaggc agaatacgcc gccgtcggcc gcgtgcgcca gctcgaccag cacgcgcgcc
     3661 acggcggcat cgtggccctc cgcgtcgtcg cgcggctcgg ggacgtcggt cggaatgccg
     3721 aacaggcact ggctgacgaa gtcgaacggt gagggaagga tctcgcgcgt cacgacgcgc
     3781 gacggcggca gcgacaggcc gagccggtct tcgagaaatc cgaaatcgtt gccggatgcc
     3841 agcgtggcgc tggtcagcac gaccgtctcg acgcggtcga acagtgcgtc cttgagcacc
     3901 gacgcgaggt cgagcggtac cgcggcgagc gacaggttcg tgcccttccg gccgcgccgc
     3961 tcgagccagc gcaccgccgg ggcgccttcg gccgcggcat cacgccccgg ccgcaggctg
     4021 gccgtgagac cgcgggcggc caggtcgagc cgccgcacca ccccgcgcag ctcgccgagc
     4081 agcgccgcag ggcgatcggc gccgtcctgc agcgagaggc ggtcggcgat agtctcgacg
     4141 ccgtcccgca ggccggcgaa ggcggccagc agattgtcca gggccacctc caatccctcc
     4201 gcccacaccg cgtcctccgt gaacgcctcg gtgaggcgca gcatcgggcc gctctggccc
     4261 gccggctcgc gctccagtcg gcggccgagc agtgtgaaca gctcttccga ccagcgccgc
     4321 gcggcccgca gcgcgtcgag cagccgctgc tccagcaggt cccggctcgc ggccgagagc
     4381 agatcgtcgc gggccgtcag ctcggcgtgc agcgccggca tcaagccccg cccgccccgc
     4441 tcgagccgac ccagcaaccg ttgcacgccc atctgcgaga cctgcgctcc gaggtgggcc
     4501 gccgcgacgt cttccaggtg atgcgcctcg tcgagaatga gccggcggta gggcggcagc
     4561 acggccgctt ccatccagtt gtccgccgcc aggcgcacgg cgaggtcgga ggcgagcagg
     4621 tgatggttca ccaccacgac atcggcttcg gcggcgcgcc gtcgcgcctg gaagacgaag
     4681 caccgctcga agtgcgggca cttgagccgc gtgcacagat cggattccgc cgcgaccgcg
     4741 tcccacacgt cctcggacgg atcgtcgggc aggtccgcga gactgccgtc ggcgctgcgg
     4801 gccgcccacg cggcgatccc ctccagctcc gtcgaccggt tggtctcgaa cagcgtctcc
     4861 tgcgcgccgc gcgcctgctc gaggcgggcg agacagagat agttccgcca tcccttgagc
     4921 agcgcgaacg tcggctcgtg gtcgggcgtg gccagggcgc gcgccagcac cggcaggtcc
     4981 ttgccgacca gctgctcctg caggttgatg gtgttggtcg agaccacggt acgctcggcg
     5041 ttcgcctgtc cccacaggag cgcgggaacg aggtacgcga acgacttgcc cacgccggtg
     5101 ccggcttcga gcagcgccac gccgccgtcg ttgtaggcat cggcgatgta ggccgccata
     5161 tcgcgttgcg cgggacggtc ctcgaacccc tcccgcccca cagcgtccgc caccggcccg
     5221 tcgatgccca gcaggcgggc ggtcgtcacc ggatcgagcg gctccaggct cttcgcgcgc
     5281 gggcattcca cgacgacgta gagctcggtc acctcgttgt tcacgatccc gaacccgacg
     5341 ccgtcgtcgt gcagccgcgc cgccaccgac agatcggccc cggacggttc gaggtccccg
     5401 ctggggtgat tgtgcagcag catctcgccc cgccgggccg agcccggcag ggcgagcacg
     5461 gcgtcgaccg tgccccgcgc cacgacacgg gcttctgcga tggcgccgcg gccgtcggcc
     5521 cgcgccacga acgagacctc acgcccccgc gccgcgacga tctccgcgcg cagcaggtcc
     5581 tgcacttcgg gcgcgagccg ctcgacgctc acgtcttcag ctcgcgcttt ttcgcagcgg
     5641 gaaacaggac gttgttgagg atcagccggt agcccggaga gtgcgtgtgg agcgagaggt
     5701 cggtcggagg atcgccgatc tggtgctgcg ggtcctcggg gtcgtgcccg ccgaagaacg
     5761 tccaggtgcc cttgcccagg tcgccgtgga tgtacttcac ccagggcgcc ccctcctcgt
     5821 cggccaggat ggtgacgccg ggcttcaggc gctcgc
//
LOCUS       DBNGZW010000683         4412 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1359303, whole genome shotgun sequence.
ACCESSION   DBNGZW010000683 DBNGZW010000000
VERSION     DBNGZW010000683.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1359303"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="AC16JC_16100"
     CDS             <1..552
                     /locus_tag="AC16JC_16100"
                     /inference="COORDINATES: protein motif:HMM:NF022135.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HIR5166273.1"
                     /translation="FLALRTGAAGTYVIGVSTRPNIIAMSGEQFREYLQEEGLDHVIA
                     ARRRAGTAQDSVRERYAKHVKAIVQVGEPRTGSFATPLGYAAELVPLDNPYAWRRGGA
                     LRFRAVVDGAPVPGLAVISGGRSAADSAIPRRALRTDAGGIVTLVPSDPGLWYLTFIH
                     IGPVSAPDRNYQSEWATITFQLR"
     gene            522..1253
                     /locus_tag="AC16JC_16105"
     CDS             522..1253
                     /locus_tag="AC16JC_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HIR5166274.1"
                     /translation="MGHDHVPAPLDPARALAALEQRVVGCVRCPRLVAYRRGVARRKR
                     RAFADQTYWGRPVPAWGDPAARLVIVGLAPAAHGANRTGRMFTGDASGDWLYAALHRF
                     GFASRPAALARDDGLRLRDCWITASSRCAPPGNKPLPAELARCRPYLSEELVLLRRRR
                     VVVVLGRIAHDAFLRAAGWWERLPARERPRFAHLAVSRLPDGTMLVASYHPSRQNTNT
                     GKLTRAMWHAVFRTAQRLLQQPAGR"
     gene            complement(1260..1556)
                     /locus_tag="AC16JC_16110"
     CDS             complement(1260..1556)
                     /locus_tag="AC16JC_16110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HIR5166275.1"
                     /translation="MANFEELPGCMVQGNSMGEVEAKLWRILPGYLRQLRRRGVAVPA
                     PLRAPPASIEGISVVLAPGGVQAPGADAGATLPLGAAGELRSVAARADVVLLGS"
     gene            complement(1650..1934)
                     /locus_tag="AC16JC_16115"
     CDS             complement(1650..1934)
                     /locus_tag="AC16JC_16115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166276.1"
                     /translation="MSGPVNVEQATALLADITRPGRTTGGIEVGALLEAWGFVHGGPV
                     DTQEPEQALFWIHPEHPHLHMTVPIGGPLSSFVVDYAAHLIRSLQDSEVA"
     gene            complement(1931..2326)
                     /locus_tag="AC16JC_16120"
     CDS             complement(1931..2326)
                     /locus_tag="AC16JC_16120"
                     /inference="COORDINATES: protein motif:HMM:NF046506.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6941 family protein"
                     /protein_id="HIR5166277.1"
                     /translation="MKATLAAIADGASVVAGDKLNVQGVFDTIFADTFPAVHARMVVV
                     VRLQLEPADDSREFTVRVRLVDDAGRRIFEQTTRTHVGHIPPGAAPATNLIYQLSNTT
                     FRGPGRYRFLIDAGSSHTDLPLRVLGRET"
     gene            complement(2438..3106)
                     /locus_tag="AC16JC_16125"
     CDS             complement(2438..3106)
                     /locus_tag="AC16JC_16125"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0017169 - CDP-alcohol
                     phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR5166278.1"
                     /translation="MADPRRELTFLLAAPERRLLRWIAARLPGWVTSDQLTALGVLAA
                     TAAAAAYALSGLHPGLLWGASAALAVNWLGDSLDGTLARVRHTERPRYGYYLDHAVDA
                     YATAAIGIGLGLSPYVSLGVALGLVVVYLWLSINVYLESTVFGAFRLAYGRLGPTEVR
                     LILIAVNTGLALHHRVPWLAGVPIRLLADGLCVALALAMFALLVNRFTRNLRDLARLE
                     PVPH"
     gene            complement(3177..3755)
                     /locus_tag="AC16JC_16130"
     CDS             complement(3177..3755)
                     /locus_tag="AC16JC_16130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166279.1"
                     /translation="MTRYLLPAILLSASSAAAQSAWRLVQTTDELTAATDTRLILRED
                     AWPTAPSKAADSFRGATLVIVCGHRLPSAGGRTLLLSADQPMQPFGDNLAYAELRFDS
                     DSASTKAYFTTLPAGSGIAVESGRSYPRYVSFLGSEQSPHFSPRIFARLIAATRLTIR
                     YRAYGTDRSATFRLTGLAEALGKLSECRWTEP"
     gene            complement(3784..4206)
                     /locus_tag="AC16JC_16135"
     CDS             complement(3784..4206)
                     /locus_tag="AC16JC_16135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR5166280.1"
                     /translation="MTEQFELRFRTIAHTALRIAAGLTYFSHGAQKLLGWFGGVGPNG
                     GTAELMSRFGAAGVIELVCGACMVLGLFTRPLALIASGEMAVAYFWVHTFRSGDLWWW
                     HNRGELVMLYSFIWLLFAAWGPGPYSLDAWIRKGRSAG"
BASE COUNT          658 a         1549 c         1604 g          601 t
ORIGIN      
        1 ttcctcgcgc tccggaccgg cgccgccggc acctacgtga tcggcgtctc gacccggccc
       61 aacatcatcg cgatgtcggg cgaacagttc cgcgagtacc tgcaggaaga aggcctggac
      121 cacgtcatcg cggcgcgccg tcgcgccggc accgcccagg actccgtccg cgagcgctac
      181 gccaagcacg tgaaggcgat cgtgcaggtc ggcgagccgc gcaccgggtc gttcgcgacc
      241 cccttggggt acgccgccga gctggtgccg ctcgacaatc cctacgcctg gaggcgcggc
      301 ggcgcgctcc ggtttcgagc cgtggtggac ggcgcaccgg tccccggcct ggccgtgatc
      361 agcggcgggc gctcggcggc cgacagcgca attccgcgcc gcgcgctgcg gacggatgcc
      421 ggcgggatcg tcacgctcgt cccttcggac cccggcctct ggtacctcac cttcatccac
      481 atcggccccg tcagcgcgcc ggaccgcaac taccagtcgg agtgggccac gatcacgttc
      541 cagctccgct agatccggcg cgggccctcg ccgcgctgga gcagcgcgtc gtgggctgcg
      601 tgcgctgccc gcgcctcgtc gcctaccggc gtggcgtggc ccggcgcaag cgccgggcgt
      661 tcgcggacca gacctactgg ggacgcccgg taccggcctg gggcgacccg gccgcccgtc
      721 tcgtgatcgt ggggctggcg ccggcggcgc acggcgccaa ccgcaccggc cgcatgttca
      781 cgggcgatgc gagcggcgac tggctgtacg cggcgctgca ccgcttcggg ttcgcgagcc
      841 ggccggcggc gctcgcccgg gacgacggac tgcggttgcg cgactgctgg atcaccgcct
      901 cgtccaggtg cgcgccgccc gggaacaagc cgctcccggc cgagctggcg cgctgtcgcc
      961 cctacctgtc ggaagagctg gtgctgctgc gacggcggcg cgtagtcgtc gtcctgggcc
     1021 ggatcgccca cgacgccttc ctgcgggcgg ccgggtggtg ggaacgactc cccgcgcggg
     1081 agcggccgcg gttcgcgcac ctggccgtct cgcgcctgcc cgacggcacg atgctggtcg
     1141 cctcgtatca ccccagccgg cagaatacga atacggggaa gctcacccgc gcgatgtggc
     1201 acgcggtgtt tcggacggcg cagcgactgt tgcagcagcc ggcgggccgc tgacgctcct
     1261 caggacccga ggagcaccac gtcggcccgc gcggcgaccg atcgcagctc gcccgccgcg
     1321 cccaggggga gggtcgcgcc cgcatcggcg cccggtgcct ggacgccccc cggcgccagc
     1381 acgacgctga tcccctcgat ggatgccggg ggggcccgga gcggggcggg gaccgcgacg
     1441 ccgcggcgcc gcagctgacg cagatagccc ggcaggatgc gccacagctt cgcctccacc
     1501 tcgcccatcg agttgccctg caccatgcag ccgggcagct cctcgaagtt ggccatccac
     1561 cactccacgc cgtccggtcg aggggagggc cagtggaggc gaacgtggta ggaggggagg
     1621 gggcgaagcc agccgaggag ggtgcgcatt caggcgactt cgctgtcctg gagcgaccgg
     1681 atcaggtgcg ccgcgtagtc cacgacgaag ctcgagagcg gaccgccgat cggcaccgtc
     1741 atgtgcaggt gcgggtgctc gggatggatc cagaacagcg cctgttcggg ctcctgggtg
     1801 tcgacggggc cgccgtgcac gaagccccag gcctcgagca gcgcgcccac ctcgattccg
     1861 ccggtcgtgc ggccgggccg cgtgatgtcg gcgagcaggg cggtcgcctg ctcgacgttt
     1921 accggtccgc tcacgtttcc ctccccagca cacgcagcgg aagatccgta tgactgctcc
     1981 cggcgtcgat caggaagcgg taccgtccgg gcccgcgaaa cgtggtgttg ctgagctggt
     2041 agatcaggtt cgtggccggc gcggcgccgg gtggaatgtg gccgacgtgc gtccgggtgg
     2101 tctgttcgaa gatgcgccgt cccgcgtcgt ccacgagccg cacgcgcacg gtgaactcac
     2161 gggaatcatc ggccggctcg agctgcaggc gcacgacgac gaccatgcgc gcgtgaaccg
     2221 ccgggaacgt atcggcgaag atggtatcga agactccctg cacgttcagc ttgtcgcccg
     2281 cgacgacgct cgcgccatcg gcgatcgcgg ccagcgtagc cttcaccaac tcctcccctc
     2341 cggatcgggc gggtaaagct ccagcgttcc cgtccggctg caagaggcgg accgcggggg
     2401 ggcacggtgc ggtgcgagcc accgcgcagg cccgctgtca gtgcggcacg ggctcgaggc
     2461 gcgccaggtc ccgcaggttg cgtgtgaagc ggttgaccag cagcgcgaac atcgcgagcg
     2521 ccagggcgac gcacaggccg tccgccagca gacggatcgg cacgcccgcg agccagggga
     2581 cacggtggtg caacgcgagc cccgtgttca ccgcgatcag gatgagacgc acctcggtgg
     2641 gtccgagccg cccgtaggcg agccggaacg cgccgaacac ggtggactcc aggtagacgt
     2701 tgatcgacag ccagagatag acgacgacca gccccagggc gacgcccagg ctcacgtagg
     2761 gggagaggcc caacccaatg ccgatcgcag cggtcgcata ggcgtccacg gcgtgatcca
     2821 ggtaatagcc gtagcgcggc cgctcggtgt gccgtacgcg cgccagcgtg ccgtcgaggc
     2881 tgtcgcccag ccagttcacg gccagcgcgg cgctcgcgcc ccagagcaat cccggatgga
     2941 gccccgacag ggcgtaggcc gcagcggcgg cggtggcggc cagcacgccg agcgccgtga
     3001 gctggtcgga ggtgacccag cccggcagcc gcgcggcgat ccagcgcagc aggcgccgct
     3061 ccggtgcggc gaggagaaag gtcagctcac gtcgggggtc ggccatgtgc gcctccggtt
     3121 gcgcgtccgg cggcacgatg acatgcccct ctcaagcggg tttcaagtgc gtggcgtcag
     3181 ggctcggtcc accggcattc ggacagcttg cccagggcct cggccaaccc cgtgaggcgg
     3241 aacgtggcgc tgcggtcggt gccgtaggcg cgatacctga tggtcagccg ggtcgccgcg
     3301 atcagcctgg cgaaaatccg gggtgagaag tggggacttt gctccgagcc gaggaatgag
     3361 acgtagcggg ggtagctccg tcccgattcg accgcgatgc cgctaccggc cggcagcgtg
     3421 gtgaagtagg ccttggtgct ggccgagtcc gagtcgaacc ggagctcggc ataggcgaga
     3481 ttgtcgccga acggctgcat cggctggtcc gcggagagca gcagggttcg acccccggcg
     3541 ctgggcaacc gatgcccgca cacgatgacg agtgtggcgc cgcggaagga atccgccgcc
     3601 ttcgagggcg cggtcggcca ggcgtcctcc cgcaggatga ggcgggtgtc ggtggcggcg
     3661 gtgagctcat ccgtggtctg caccagccgc cacgcggact gggcagccgc gctggacgct
     3721 gagagaagga tcgcgggcag caggtacctg gtcatgggtg tcggaccggc tggcgggatg
     3781 cgctcagccg gcgctccgtc ccttgcggat ccaggcatcc aggctgtacg gcccgggtcc
     3841 ccaggccgcg aagagcagcc agatgaacga atacaacatc acgagctcgc cgcggttgtg
     3901 ccaccaccag aggtcgccgc tgcggaacgt gtgcacccag aaataggcga ccgccatttc
     3961 tcccgaggca atgagcgcca gcggccgggt gaacaggccg aggaccatgc acgcgccgca
     4021 caccagctcg atgaccccgg ccgcgccgaa ccggctcatc agctccgccg ttccgccgtt
     4081 ggggccgaca cccccgaacc agccgagcag cttctgcgcc ccgtgtgaaa aatatgtcag
     4141 gccggcggcg atgcggagcg cggtgtgggc gatggtgcgg aaccggagtt cgaattgctc
     4201 ggtcacgggc ccctcccggg gttatcttgt gatggcggcg gctcgggtat aatcgtgccc
     4261 gccccgctcc ccccacgcaa ggcgggggcg cgtcgtccga ccctttcgca agaggtatcg
     4321 cacgcatgaa ccggatctcc ttcgtgtctg ccgccctgct gctcgtcggc ggcggcctgg
     4381 cggcacagca gaacgacccc gaccgctcgg tg
//
LOCUS       DBNGZW010000684        16907 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_609621, whole genome shotgun sequence.
ACCESSION   DBNGZW010000684 DBNGZW010000000
VERSION     DBNGZW010000684.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 16907)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16907)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16907
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_609621"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..951
                     /locus_tag="AC16JC_16140"
     CDS             <1..951
                     /locus_tag="AC16JC_16140"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF027964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HIR5166281.1"
                     /translation="LRATLRGHAAVMNALPYYYNLDVTRAAVEVGLHYADLGGNTEIV
                     FNQKKLDAEAATRQVSVIPDCGLAPGMVNILAAEGIRRVADAESVKIYVGGLPQHPEP
                     PLNYQIVYSLEGALDYYTTPSWVLRDGKPARVDALSELETLVFPPPVGTLEAFHTGGG
                     ISTMPWAYEGRVRTMEYKTLRYPGHMAIMRPIRELGLLDNTPIKVKGKEIVPRDAFIA
                     AVSPKLTKPQGRDLVALRVDVHGAGGKRASWELLDYYDAARGISAMMRTTGYSLSITG
                     LMQADGRITARGVHTPDEAVPFEAYIGELKKRGVEIREVA"
     gene            1021..1587
                     /locus_tag="AC16JC_16145"
     CDS             1021..1587
                     /locus_tag="AC16JC_16145"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR5166282.1"
                     /translation="MDFRALWERALPYHQFVQEASGNHDLWTGMYRIARIPDWALAAA
                     RSTGTKHHLLVLAEDWCGDGTNTVPYLAKLADLSRSQEVRVLRRDEHPTVMDRYLTAG
                     TRAIPIVIVLDRQFREVGHWGPRPEALQAWVQEARKSVDRARLYPQIRRWYAKDKGES
                     TLREVLGLLERMPSAVSYQLSAETRMTG"
     gene            complement(1622..2767)
                     /locus_tag="AC16JC_16150"
     CDS             complement(1622..2767)
                     /locus_tag="AC16JC_16150"
                     /inference="COORDINATES: protein motif:HMM:NF013283.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HIR5166283.1"
                     /translation="MSLRRVAPLLVAALGGSRLAAQTARESTMWDYTVTPYGYYSTID
                     GWWLAGYGRVYSPIGFTERPEPNRAALTLTGGASTEGSHLVELDAQAPALWAGWRVGL
                     TVDALRANRLGYFGIGNATAYDRDSITPTSPYFYGVSRSSKLARLTIQRRVAGPLRVL
                     VGGTLEHTSYRTLPGDGLFARDRAAGVADTAPFDDAALRAGLVADSRDNELDPHSGVL
                     AEALLAGARRYSRATVGVQAYVHPFERLVLAGRVLGERMSGAPSISTMQVIESSGRPY
                     VGVGGYRSLRGYYDSRFIGPGKLLGSFEVRYFLINAPTIAELKVVGFYDVGRVFGPGV
                     LSFTTRGLHSSAGGELMLRLGRNSVLVAGAGFGEDGGQFLFGSTWSY"
     gene            complement(2764..3825)
                     /locus_tag="AC16JC_16155"
     CDS             complement(2764..3825)
                     /locus_tag="AC16JC_16155"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013292.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosylasparaginase"
                     /protein_id="HIR5166284.1"
                     /translation="MTQDVSRREFLASTANGLAAAGTLGIPHPLRPPHPLPPRRAWAV
                     QPVVISSANSLRPVQKAYDLLLQGADPLDAVVEGVKIQELDPNDQSVGYGGLPNADGV
                     VQLDASCMHGPSKRAGAVGALEGIKTPSEVAKLILKYTNHIYLMGEGAKRFALSYGFK
                     DEDLLTDASRQAWLEWRANLSPTDNWRDVPGNTPMVVHPPTGTINCDAVTPAGDIASV
                     TTTAGLAWKIPGRVGDSPIIGAGQYCDNEIGAAGSTGLGEVNIMVCGAFLTVDQMRRG
                     MKPTDACLETLRRAVRLSEPRLLTPDGKPRYQLNFYAVNKRGEFGSAALYPSRFAVHD
                     GTQARFVDAAHLYEGTVPS"
     gene            3927..4676
                     /locus_tag="AC16JC_16160"
     CDS             3927..4676
                     /locus_tag="AC16JC_16160"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015752570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR5166285.1"
                     /translation="MSPYDAAKAHPLDVSIVIPVYDERDNLGPLMAEIAAAMAGGGRC
                     YEIVAVNDGSTDGSLDVLKALKRTHPELHIVSFAANAGQTAAFAAGFRAAHGAVIVTM
                     DADGQNDPADVPALLEALERSSASAVAGYRVRRHDSVWKRVQSRIANGVRNRLNHETI
                     RDTGCSLKAFRAAALGELPLFTGMHRFLPTLVRMHGGTVTEVAVRHRPRRSGRTEYGM
                     WNRVFRSLADAFAVRWMQRRQLRYRVSEEIP"
     gene            4688..5764
                     /locus_tag="AC16JC_16165"
     CDS             4688..5764
                     /locus_tag="AC16JC_16165"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HIR5166286.1"
                     /translation="MPRPSPASRKPRLDPAIFNLPVARMREGYYSDKYFVRARDVLIA
                     DRHRPRVTMQVFGKSHAYLGGSDEAIAILKLCAQSWEEMEVHALYDGDEVAPWETVLL
                     IEGPYDAFANLETLYLGVLARRTKVGTNTRLVVEAARPKQVMFFPARHDHWLVQTGDG
                     YAAHIAGAIGVSTDAQASWWGSEGIGTVPHAMIAAYGGDTVRASVKFAEYMDADTRLI
                     TLVDFDNDCVTTSLEVARALGERLYGVRLDTSETLVDKAIVPEMGTFRPTGVNPQLVW
                     KVRQALDREGYASVKIVVSGGFTVEKIRAFEEEGVPVDMYGVGSSLFQGRFDFTADIV
                     QVEGKPCAKVGRGYRPNPRLERVT"
     gene            5778..6770
                     /locus_tag="AC16JC_16170"
     CDS             5778..6770
                     /locus_tag="AC16JC_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166287.1"
                     /translation="MQAASHKKIVFGAIGCSGLLVLLVVASLIMARRSGVAPPPSELP
                     LAPVTAAAALDSLTNYDLRALFALPDAPFVLVSSGATMFARWRPDRRRLAVQFLDTAP
                     PDSRQGRIWREVGAAVAIESLPAGAPPPDSQPIGRAILAAARVQLESGDPARAREAVV
                     LAVRRARAFEGRPDILLVVSGLRLERDALEMLARDSLLAGGASARALAEASLVAMDRR
                     LGEVRSVRSLIATAGTSPSAVPTLAGWARDPTLPLAFRDEMIRAVGFGWVLNPQEVLE
                     GLDTARAAAIRTLAAGTWPSSLAQTLQEVRPGSPNAVARLRLSMSYRAKRMLDL"
     gene            6855..7520
                     /locus_tag="AC16JC_16175"
     CDS             6855..7520
                     /locus_tag="AC16JC_16175"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase family protein"
                     /protein_id="HIR5166288.1"
                     /translation="MARVIFWDVDTQYDFMRADGKLYVPDAEHIIPNLKRLTDYAHGH
                     GIRVVASADDHVEGHRELSDTPDFKETFPLHCMRGTPGQRKIPETALREALVVEPEPL
                     VPASLADQMRSHAGDILFHKHWFDVFTNPNVLPMVDALAPEDIVLYGVALDVCNRYAI
                     EGFLERRPNIRLFAVTDAMKPIDRDVGEQMLREWGDEGVRLVKTSEVVFGGLVEQLAQ
                     ADA"
     gene            7517..8593
                     /locus_tag="AC16JC_16180"
     CDS             7517..8593
                     /locus_tag="AC16JC_16180"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR5166289.1"
                     /translation="MSAAFGKFFLPGPTDVHPDVLAAQLRPMIGHRSGAMEQLLQKMA
                     PELQALARTSRTVLIGTTSATGFMEMAVRNGVRQRALSIVNGAFSERFAKLVTATGKE
                     CVRLDVPLGEAVEPDQLRDALKRTPVDAVTLAHSETSTGVLQDVAALAKVVHEFDDVL
                     VLVDAVTSMAGSPVETDAWGFDFTFTGSQKALALPPGLALGIASQRMLERSKTLPARG
                     LYFDLPSFEQAMSKFQPTNTPALSLCFALDRQLDRIAAEGGIEARWKRHEAMRRTVER
                     WAQARDIAYLPREGRRSWTVSCLRLPAGKSAKDVVGKLKAQGWTIGSGYGALKDSSIR
                     IGHMGDHSVAAVEELLPLVGQALA"
     gene            8590..10179
                     /gene="serA"
                     /locus_tag="AC16JC_16185"
     CDS             8590..10179
                     /gene="serA"
                     /locus_tag="AC16JC_16185"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: protein motif:HMM:TIGR01327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR5166290.1"
                     /translation="MTLRVVVADKMAPVGLELLAGTPGIEVANCAGKPREELERALEG
                     GHALIVRSETRVTADLLNRAPQLRVIARAGIGVDTIDVTAATRRGIAVMNAPGANTVS
                     AAEHAMALLLALVRHIPWAAESMRRGEWDRKKYEGTELRGKTMGIVGLGRIGGHVSQI
                     ARAFGMNVVGHDPFLPPERAAELQVKILPLEQLLASADVVTLHVALSDQTRHLINAER
                     LAAMKKTAVLINTARGELVDEAALVAAVTNKQIRGAAVDVFAQEPLPADSALRTLDRV
                     ILTPHLAASTAEAQDRVSQEICVAVRDALLAGDLSTAVNVPGIGGEVLRRAAPLLVLA
                     RRLGRLAAGLAAGEVTAVEVTYGGKDEEAARPMLVAALEGLLAGLGAGPVSMVNAPVL
                     AEERGIRLTRRSGAPEPGFETSIGVKVEARSGRVRVAGALVGSHGRVIRIDDYHVDVA
                     PEGWLLVVRNRDVPGVIGRVGTMLGECGVNIASWHQGRDAREGEALAAITVDQPPVDE
                     VRARLEKLPDILEVRVANFGE"
     gene            complement(10269..11639)
                     /locus_tag="AC16JC_16190"
     CDS             complement(10269..11639)
                     /locus_tag="AC16JC_16190"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR5166291.1"
                     /translation="MRSRIPAALVILAALGSAGLSAQDPGTLELGAFGRFTRFDRTLG
                     LDNSFGGGGRLGVFVAQGLALEGTGGYLPTTGPFVADSRVIPLHARLVYARPIAGAVA
                     VLVGGGYVHNVYGTTGNVSDDGVSGLFGLRFGMGPSFAMRIEAIEDFIPSPNNQAAGV
                     PNNWNFTVQAGLSTWMGRSHPKDSDGDGIVDRLDSCPATPPREAVDDKGCALPKDSDA
                     DGIVDRLDRCADTPAGDRVDASGCSLPKDADGDHVADDADRCPNTAPGEAVDAAGCPR
                     DSDRDGIVDSVDKCANTPAGAIVDATGCPPPTDADRDGIVDANDKCPGTPGGERVDSI
                     GCPALFVGAERTLVLEGVTFETGKTVLTAQARGALERVALSLKGNPGQRVEVAGYTDS
                     RGTAGANLRLSQARADAVRGYLIERGVPPEQVVSRWYGEDDPVDTNATAGGRARNRRV
                     ELHRVN"
     gene            complement(11797..13167)
                     /locus_tag="AC16JC_16195"
     CDS             complement(11797..13167)
                     /locus_tag="AC16JC_16195"
                     /inference="COORDINATES: protein motif:HMM:NF012894.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HIR5166292.1"
                     /translation="MRAARRLAAIVTLAASPAAAQLPGTIELGPFARFTRFDQSLTFD
                     NTIGGGGRLDVFVMRNLGVEAAAFVQSTTTAGGDGVSLVPLELRLLYAAPVAASAAVL
                     VGAGYVHQTYRKSLHGWEDGISGLLGVRVDLGNRLVGRLEGVADYFPSPLNTTVGVQD
                     NWDFSIQAGLGVQLGGVAGGVRDSDHDGVVDDADACPGTPRGEAVDGRGCPLPKDADA
                     DGVVDANDRCHDTPAGEHVDTDGCTLPMDADGDGVVDGNDRCPATPAAVQVDAAGCPL
                     DTDRDGVPDMTDKCPTTPPGDQVDAVGCSLPKDADQDGVLDAVDKCPSTPPGERVDAA
                     GCRILFVGAERTLVLEGVNFETASSNLTDQSHNTLDRVAASLQAYPTLRVEVAGYTDS
                     RGSLATNRRLSQSRADAVRAYLIAQGVDPLQLTARGYGPTSPVASNATETGRARNRRV
                     ELHRLN"
     gene            complement(13164..15350)
                     /locus_tag="AC16JC_16200"
     CDS             complement(13164..15350)
                     /locus_tag="AC16JC_16200"
                     /inference="COORDINATES: protein motif:HMM:NF012245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HIR5166293.1"
                     /translation="MATTLRDLLQRFSMSVAAAALIVLLGLGLLAARSARRSLERLSD
                     QHGTEVASRAADLVATYIRDRHREADRLATNPFVVRAALEAGQTVSQRRLDQSEPATL
                     ERLFGATHQIGGDRNLENYFKRYPDGSDFTDLAVTERHGFTVMASSRPDRFSRASDPL
                     WQQAMQQDAAETTPAVDSASQSVTVRYAVAIRPAEGAQAVGVLEAIYQLDRLGLLLGR
                     SGLGDSAYLQLVDERGNLLFGPEQAELRGVPRDRALFNPDQPRRTVLEASRGAELVVS
                     VPVARGQFPANQSRIWVLFHQPTVTAYTLSRAVQRTVLVAAVILFGLAVAAMLWIGRW
                     LNQRIAAPVRAAGEIAARVAGGDLSVTDTARQSEAGEIGDLTTSVQRMVAALRQLVGA
                     IRSASDESAAMAAEISAATQQMSASTEEMTSTTQDLTKRAAEQAQLVRAAADDAGRIL
                     QIATALAAGAEDSVRRNAELAGLARHHREQLDQSITQLTRLAEEVERWADDADALATS
                     SAQIQKFVGQAKAVATQTNMLALNAAIEAARAGPQGRGFAVVADEVRKLASLAAAAAT
                     ETADTVRSVLTRAQETRDRLQRLAQTGAVVRETAKTASQGLSLVAGEAEANDAWSQEI
                     ATSAVEVRHLVDEIAARLAAVAMGTDGLLASAQEIAASSQQQSASTEEIASSANQLAE
                     AADRLQGAVKSFRIVAEQPAPPPAAPAPPPDDQPLPGPSLAPEVAT"
     gene            15414..>16907
                     /gene="leuS"
                     /locus_tag="AC16JC_16205"
     CDS             15414..>16907
                     /gene="leuS"
                     /locus_tag="AC16JC_16205"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR5166294.1"
                     /translation="MSHVPVYDPQAVEARWQQRWVERHTNEPELDRPARPFYNLMMFP
                     YPSAEGLHVGNMFAFTGADIFGRFQRLQGYDVFEPIGFDAFGIHSENYALSVGTNPAQ
                     LIPRNIDTFRRQLRRFGGMFDWRHELSTTDPAYYQWTQWLFLQLFKANLAYKKRAAVN
                     WCPKDKTVLANEQVINGRCERCDTPVEQRSLDQWFFRITDYAERLLRNLDTLDWSEST
                     LALQRNWIGRSEGAELVFTTPAGQTITVFTTRPDTVFGATYLVLAPEHPLVEALTAGE
                     QRTAVRAYQREVQSRDVVARRVGEKVKTGVFLGSYARNPATGEAIPIWIADYVLMEYG
                     TGAIMAVPAHDERDHAFASQFGLPIRQVVNVAGAASELPSVSEDGVLMNSGRFDGLAC
                     REGGKQIVAWLAEQQVGSPRVQYRLHDWCISRQRYWGPPIPIIYCDRCGPVPVPERDL
                     PVLLPLIEDFRPDDTGVSPLARVESWYQVACPQCGKPARRETDVSDTF"
BASE COUNT         2484 a         5861 c         6002 g         2560 t
ORIGIN      
        1 ctgcgggcga ccttgcgcgg gcatgcggcg gtgatgaacg cgctgcctta ctactacaat
       61 ctcgacgtca cgcgggccgc ggtcgaggtc gggctgcact acgccgacct gggcggcaac
      121 accgagatcg tcttcaatca gaagaagctc gacgccgagg ccgccacccg acaggtgtcg
      181 gtgatccccg actgcggcct cgcccccggc atggtgaaca tcctcgccgc cgagggcatc
      241 cgtcgcgtgg ctgacgcgga gtcggtgaaa atctacgtcg gtgggctgcc gcagcacccc
      301 gaacctccgc tcaactacca gatcgtgtac tcgctcgagg gcgcactcga ctactacacg
      361 actccctcct gggtgctgcg cgacggcaag ccggcgcgcg tcgatgcgct ctccgagctc
      421 gagacgctgg tctttccgcc acccgtcggt accctcgagg cgttccacac cgggggcggg
      481 atctcgacca tgccgtgggc ctacgaaggc cgggtgcgca cgatggagta caagaccctg
      541 cgttacccgg ggcacatggc catcatgcgt ccgatccgcg agctcggact gctcgacaac
      601 accccgatca aggtgaaggg caaggagatc gtgccgcgcg acgccttcat cgccgccgtc
      661 tcgccgaagc tcacgaagcc gcagggccgc gacctcgtcg ccttgcgcgt ggacgtccac
      721 ggcgcgggcg ggaagcgggc gtcctgggaa ttgctcgact attatgacgc cgcgcgcggc
      781 atcagcgcga tgatgcgcac caccgggtac tcgctgtcca tcaccggact gatgcaggcg
      841 gacgggcgga tcacggcgcg gggcgtgcac acccccgacg aggcggtgcc gttcgaagcc
      901 tatatcggcg agctgaagaa gcgcggcgtg gagatccggg aagtcgcctg accacgacgt
      961 gaagaggcct tcacgttgcc ttgtaagtgc cttcccttcc ggaaagcttc cgttcagcgc
     1021 atggactttc gcgcgctgtg ggagcgggcg ctcccctacc accagttcgt ccaggaagca
     1081 tccggcaacc acgacctgtg gacggggatg taccgcatcg cccgcattcc cgactgggcc
     1141 ctggcggccg cccgctccac cggaaccaag caccatctcc tggtgctcgc ggaggactgg
     1201 tgcggggacg gcacgaacac cgttccctac ctggcgaagc tcgccgacct gtcacgcagc
     1261 caggaggtgc gggtgctgcg ccgtgacgag catcccacgg tgatggaccg ctacctcacg
     1321 gcgggcacgc gcgcgattcc catcgtgatc gtgctggacc gccagttccg cgaggtgggg
     1381 cactggggac cgcgccccga ggcgctgcag gcctgggtgc aggaggcccg caagagcgtc
     1441 gaccgggcca ggctctaccc gcagattcgc cgctggtacg cgaaggacaa gggcgagtcg
     1501 acgctgcggg aggtgctggg gctgctggaa cggatgccgt cggctgtcag ctatcagcta
     1561 tcggctgaaa cacggatgac tggctgatag ccgatagccg atagccggta gccgacagcc
     1621 gtcagtagct ccacgtactc ccgaacagga actgcccccc gtcttcgccg aagccggccc
     1681 cggcgaccag cacgctgttg cgtcccaaac gcagcatcag ctcgccgccg gcgctcgagt
     1741 gcagtccgcg cgtcgtgaag ctcaacacgc cgggtccgaa cacgcggccg acgtcgtaga
     1801 atcccaccac cttcagctcg gcgatcgtgg gcgcgttgat caggaaataa cgcacctcga
     1861 agctccccag cagcttgccg gggccgatga agcgcgaatc gtagtagccg cgcagcgagc
     1921 gatacccgcc cacgcccacg taggggcgcc ccgacgattc gatcacctgc atcgtcgaga
     1981 tgctgggcgc gccgctcatg cgctccccca ggacccggcc ggcgagcacc agccgttcga
     2041 agggatggac gtacgcctgg accccgacgg tcgcacggct gtagcgccgc gccccggcga
     2101 ggagcgcctc ggcgagtaca ccggagtgcg ggtcgagctc gttgtcgcgc gaatcggcca
     2161 cgaggccggc gcggagcgcg gcatcgtcga acggcgcggt gtccgccacg ccggccgcgc
     2221 ggtctcgtgc gaacaacccg tcgcccggga gcgtgcggta ggacgtgtgc tcgagcgtcc
     2281 cgcccactag gacgcggagc ggacccgcca cgcggcgctg gatcgtgagc cgggcgagct
     2341 tcgagctgcg gctcacgcca tagaaatacg ggctcgtcgg cgtgatgctg tcccgatcat
     2401 aggcggtggc gttgccgatg ccgaaatacc ccaggcggtt ggcgcgcagg gcgtcgacgg
     2461 tgagacccac gcgccacccc gcccacagcg ccggcgcctg ggcatccagc tcaaccaggt
     2521 ggctgccctc ggtgctcgcc ccaccggtga gcgtgagcgc ggcgcggttc ggctcgggtc
     2581 gctcggtgaa cccgatgggg ctgtagacgc gcccgtagcc cgccagccac cagccgtcga
     2641 tggtgctgta gtagccgtag ggagtgaccg tgtaatccca catggtcgac tcccgggctg
     2701 tctgggcggc caggcgggag ccgcccagcg cggccacgag caacggcgcg actcgtcgca
     2761 ggctcaactg gggaccgttc cttcgtagag atgtgccgcg tccacgaacc gggcctgtgt
     2821 cccgtcgtgc accgcgaagc ggctcgggta gagcgccgcc gagccgaact cgccacgctt
     2881 gttgaccgcg tagaagttga gctggtagcg cggcttcccg tccggcgtga ggaggcgcgg
     2941 ctcgctcagc cgcacggcgc gccggagggt ctcgaggcag gcatcggtgg gcttcatgcc
     3001 gcgccgcatc tggtccacgg tcaggaacgc gccgcacacc atgatgttca cctcgccgag
     3061 cccggtcgag cccgcagcgc cgatctcgtt gtcacagtac tggcccgcgc cgatgatcgg
     3121 gctgtcgccc acccggccgg ggatcttcca ggcgagcccg gccgtggtgg tgaccgacgc
     3181 gatgtcgccc gccggcgtga cggcatcgca gttgatggtg cccgtcggcg ggtgcactac
     3241 catgggggtg ttccccggca cgtcgcgcca gttgtcggtg gggctcaggt tggcgcgcca
     3301 ctcgagccag gcctgccgcg aagcgtcggt gagcaggtct tcgtccttga agccgtagga
     3361 gagggcgaac cgcttggcgc cctcgcccat gagatagatg tggttcgtgt acttgaggat
     3421 gagcttcgcg acctcggagg gcgtcttgat gccttcgagc gcgcccacgg cgccggcccg
     3481 cttgctggga ccgtgcatgc aggatgcgtc gagctgcacg actccgtccg cgttgggcaa
     3541 cccgccgtat cccaccgact gatcgttcgg gtcgagctcc tggatcttca cgccctcgac
     3601 caccgcgtcg agcggatcgg cgccctgcag caggaggtcg tacgccttct ggaccggccg
     3661 caggctgttg gcggagctga tgacgaccgg ctgcaccgcc caggcgcgcc gcgggggcag
     3721 cgggtgcggg ggccgcagcg ggtgggggat accgagcgtc ccggccgcgg cgaggccatt
     3781 cgccgtcgac gcgaggaact cgcggcgcga aacgtcttga gtcacggacg actccgatgt
     3841 ggtggcgagt ggacggatgg tgttttagcc ccggccctcg accctgtcaa cttgtggaag
     3901 cccggagcgg attctacgtt ctggccgtgt ctccgtacga cgcagccaag gcccatccgc
     3961 ttgacgtttc catcgtcatc ccggtgtacg acgagcgcga caacctcggc cccctgatgg
     4021 ccgagatcgc ggcggcgatg gcgggagggg gccggtgcta cgagatcgtg gcggtgaacg
     4081 acggctcgac cgacggttcg ctcgacgtgc tcaaggccct gaagcgcacg caccccgagt
     4141 tgcacatcgt gtcgttcgcc gccaacgccg gtcagacggc cgccttcgcc gcggggttcc
     4201 gggcggcgca cggggcggtg atcgtgacca tggacgccga cggccagaac gatccggcgg
     4261 acgtcccggc actgctcgag gcgctggagc gcagctccgc gtccgcggtc gcggggtacc
     4321 gcgtgcggcg ccacgactcc gtgtggaagc gggtgcagag ccgcatcgcc aacggggtgc
     4381 gtaaccggct gaaccacgag accatccgcg acaccggctg ctccctcaag gcgtttcgcg
     4441 ccgcagcgct gggcgagctg ccgctgttca cggggatgca ccgcttcctg ccgacactcg
     4501 tcaggatgca cggcggtacg gtgaccgaag tcgccgtgcg ccaccggccg cgccgcagcg
     4561 gccgcaccga gtacgggatg tggaatcggg tattccgttc cctcgccgac gcgttcgccg
     4621 tgcgctggat gcagcgccgc cagctgcgtt accgcgtctc cgaagagatt ccctaaccag
     4681 gaaccccatg ccccgccctt ctcccgcctc gcgcaaaccc cggctcgacc cggcgatctt
     4741 caacctgccg gtggcccgga tgcgcgaagg ctactactcc gacaagtact tcgtgcgcgc
     4801 ccgcgacgtg ctgatcgcgg accggcaccg gccccgcgtc acgatgcagg tgttcggcaa
     4861 gtcgcacgcc tacctgggcg gatcggacga agccatcgcc atcctcaagc tgtgcgccca
     4921 gtcgtgggag gagatggagg tgcacgccct gtacgacggg gatgaggtcg ccccgtggga
     4981 gaccgtcctg ctgatcgagg gcccgtacga tgccttcgcg aacctcgaga cgctgtacct
     5041 cggcgtgctc gcccgccgca ccaaggtcgg gaccaacacc cgcctcgtgg tggaggcggc
     5101 gcgaccgaag caggtcatgt tcttccccgc gcgccacgac cactggctgg ttcagaccgg
     5161 cgacggctac gccgcgcaca tcgccggcgc catcggcgtg tccaccgacg cgcaggcgtc
     5221 gtggtggggg tcggagggga tcggcaccgt cccgcacgcg atgatcgcag cctacggggg
     5281 ggataccgtg cgggcctcgg tcaagttcgc ggagtacatg gacgccgaca cgcggctcat
     5341 caccctggtg gatttcgaca acgattgcgt caccacgtcg ctcgaggtgg cccgcgcgct
     5401 gggcgagcga ctgtacggcg tccggctcga cacgtcggag acgctggtgg acaaggcgat
     5461 cgttccggag atggggacct tccggccgac cggcgtgaat ccccagctcg tctggaaggt
     5521 ccgtcaggcc ctggatcgcg aggggtacgc gagcgtgaag atcgtcgtgt ccggcggatt
     5581 cacggtggag aagatccgcg cgttcgagga agagggcgtc ccggtggaca tgtacggcgt
     5641 cggctccagc ctgttccagg gacgcttcga cttcacggcc gacatcgtcc aagtggaagg
     5701 gaagccctgc gccaaggtgg gacgcggcta ccggccgaat ccccggctgg agcgggtcac
     5761 gtaagggagg cgcgaccatg caggcggcct cccacaaaaa gatcgtcttc ggcgcgatcg
     5821 gctgtagcgg tctcctcgtg ttgttggtgg tggcatcgct gatcatggcg cgacgcagcg
     5881 gtgtcgcacc acccccgtcc gagctccccc tcgctccggt cacggcggcc gccgcgctcg
     5941 actccctcac caactacgac ctccgggcgc tgttcgcgct gccggatgct ccgttcgtgc
     6001 tcgtgtcctc cggcgcgacc atgttcgccc ggtggcgtcc cgatcgtcgt cgtctcgccg
     6061 tccagttcct cgataccgct cctcccgatt cccggcaggg ccggatctgg cgcgaggtcg
     6121 gggcggccgt ggccatcgaa tcgctgccgg ccggcgcacc gccgccggat tcccaaccca
     6181 tcggtcgcgc catactcgcc gcggcccggg tgcagctcga gtccggcgat ccggcccgtg
     6241 cccgggaggc ggtggtcctg gcggtgcggc gcgcgcgtgc cttcgaggga aggccggaca
     6301 tcctcctggt cgtttccggg ctgcggctgg agcgggacgc tctggagatg ctcgcgcgag
     6361 actcgctgct cgccggtggc gcgtccgcgc gggccctggc ggaggcgtcg ctcgtcgcca
     6421 tggaccggcg cctgggcgag gtgcggtccg tgcgctcgct gatcgccacg gccggcacgt
     6481 cgcccagcgc cgtcccgacc ctggcggggt gggcgcggga ccccaccctg ccgctggcgt
     6541 tccgggacga gatgatccgc gccgtggggt tcggatgggt gctcaacccc caagaggtat
     6601 tggagggtct ggacacggcc cgcgccgcgg ccatccggac cctggccgcc ggcacgtggc
     6661 cgtcgtcgtt ggcgcagacc ctgcaggaag tgaggccggg cagtccgaat gcggtggcgc
     6721 gcttgaggct ctccatgtcc tatcgcgcca agcgcatgct ggatttgtga agccccgcgc
     6781 cacggctccg gttgacacgt cccgcatccc ccccagagat tccccacgcc gcctttttct
     6841 ctccgaggag cagcatggcg cgggtcattt tctgggacgt cgacacccaa tacgatttca
     6901 tgcgggcgga cggcaagctg tacgtgccgg acgccgagca catcattccg aacctcaagc
     6961 ggctcaccga ctacgcccac gggcacggga tccgggtcgt ggcgagcgcg gacgatcacg
     7021 tcgagggaca ccgggaactg tccgacacgc cggacttcaa ggagacgttt ccgctgcatt
     7081 gcatgcgcgg cacgcccggt cagcggaaga ttcccgagac cgcgctgcgc gaggccctgg
     7141 tggtcgagcc cgagccgctg gtgccggcgt cgctggccga tcagatgcgc agtcatgcag
     7201 gcgacatcct gtttcacaag cactggttcg acgtcttcac caaccccaac gtcctgccca
     7261 tggtcgacgc cctggccccc gaggacatcg tgctgtacgg cgtggcgctg gacgtctgca
     7321 accgctacgc catcgaggga ttcctcgagc ggcgccccaa catccggctg ttcgcggtga
     7381 ccgacgcgat gaagccgatc gaccgtgacg tgggtgagca gatgctgcga gaatggggcg
     7441 acgagggcgt gcgcctggtg aagacgagcg aggtcgtgtt cggcggcctg gtggagcagc
     7501 tggcgcaggc ggacgcgtga gcgccgcctt cgggaagttt ttcctccccg gcccgaccga
     7561 cgttcacccc gacgtcctcg ccgcccagct gcgccccatg atcggccacc gctcgggggc
     7621 gatggagcag ttgctgcaga agatggcgcc cgaactccag gcgctcgccc gcacgtcgcg
     7681 cacggtcctg atcggcacca cctcggcgac cgggttcatg gagatggccg tgcgcaacgg
     7741 agtccgccag cgcgccctct cgatcgtgaa cggcgcgttc agcgagcgct tcgcgaagct
     7801 cgtcacggcg accggaaagg agtgcgtgcg gctggacgtg ccgctgggag aggcggtcga
     7861 gccggaccag ctgcgcgacg ccctgaagcg gaccccggtg gatgccgtca cgctggcgca
     7921 ctcggagacg tcgaccggtg tgctgcagga cgtcgcggcg ctcgccaagg tggtgcacga
     7981 gttcgacgac gtgctggtcc tggtggatgc ggtcacctcc atggccggca gtccggtcga
     8041 gaccgacgcc tggggcttcg acttcacctt caccggatcg cagaaggcgc tggctctgcc
     8101 gccgggcctg gccctcggta tcgcgtcgca gcggatgctg gagcgctcca agaccctccc
     8161 ggcgcgcggc ctctacttcg acctgccgag cttcgagcag gcgatgtcca agttccagcc
     8221 gaccaacacg cccgcgctct cgctctgctt cgcactcgac cggcagctcg accggatcgc
     8281 cgccgagggc ggcatcgagg cgcgctggaa gcggcacgag gcgatgcgcc ggaccgtcga
     8341 gcgctgggcg caggccaggg acatcgccta tctgccgcgc gaggggcgcc gcagctggac
     8401 ggtgagctgc ctcaggcttc ccgccggcaa gagcgccaag gacgtggtcg gcaagctcaa
     8461 ggcgcagggg tggacgatcg ggtcgggcta cggggccctc aaggactcgt ccatccgcat
     8521 cggtcacatg ggcgatcact cggtcgccgc ggtcgaagag ctgctgccgc tcgtggggca
     8581 ggcgctggca tgaccctccg cgtcgtggtc gccgataaga tggcccctgt gggcctggag
     8641 ctcctggccg gcacgcccgg gatcgaggtc gccaattgcg ccggcaagcc gcgcgaggag
     8701 ctggagcggg cgctggaggg cggccacgcc ctcatcgtcc gcagtgaaac gcgcgtcacc
     8761 gccgacctgc tgaaccgcgc accgcagctc cgggtcatcg cccgggccgg gatcggcgtg
     8821 gacacgatcg atgttacggc cgcgacgcgc cgcggcatcg ccgtgatgaa cgcgcccggg
     8881 gccaacaccg tgagcgccgc cgagcacgcc atggcgctgc tcctcgccct cgtccgccac
     8941 atcccgtggg cggcggagtc gatgcggcgc ggggaatggg accgcaagaa gtacgagggc
     9001 accgagctgc gcggcaagac gatgggcatc gttggtctgg gccggatcgg gggtcacgtg
     9061 tcgcagatcg cccgcgcctt cgggatgaac gtcgtgggcc acgatccgtt tctgccgccc
     9121 gagcgcgccg ccgagctgca ggtcaagatc ctcccgctcg agcagctgct ggcgagcgcg
     9181 gacgtggtca cgctgcacgt cgcgttgagc gaccagacgc gccatctgat caacgccgag
     9241 cggctggcgg cgatgaagaa gaccgccgtg ctgatcaata cggcgcgcgg tgagctggtg
     9301 gacgaggccg cgctcgtcgc ggcggtcacc aacaagcaga tccggggcgc cgcggtggac
     9361 gtcttcgccc aggagccgct gcccgcggac tcggcgctgc gcacgctcga ccgagtgatc
     9421 ctcacgccgc acctcgcggc ctccacggcc gaggcgcagg accgggtgtc ccaggagatc
     9481 tgcgtcgcgg tgcgcgacgc cctgctcgcg ggtgatctct cgaccgctgt caacgtgccc
     9541 ggtatcgggg gcgaggtcct gcggcgcgcc gcgccgctgc tcgtgctggc ccgacggctg
     9601 gggcgcctgg cggcggggct cgccgcgggg gaggtgaccg cggtcgaggt cacctacggc
     9661 ggcaaggacg aagaagcggc ccggccgatg ctcgtggcgg ccctcgaggg cctgctcgcg
     9721 ggcctgggcg cggggccggt gagcatggtc aacgctccgg tgctggcgga agagcgcggc
     9781 atccgtctca cgcggcgcag cggcgccccc gagcccgggt tcgagacctc gatcggcgtc
     9841 aaggtcgaag cgcgcagcgg ccgcgtgcgt gtcgcggggg ccctcgtggg aagccacggc
     9901 cgggtcatcc ggatcgacga ctaccacgtg gacgtggcgc ccgaggggtg gttgctggtg
     9961 gtcaggaacc gcgacgtccc gggcgtgatc gggcgggtgg ggacgatgct gggcgagtgc
    10021 ggcgtgaaca tcgcgagctg gcaccagggc cgcgacgcgc gcgaaggcga ggccctcgcc
    10081 gcgattacgg tggaccagcc gccggtggac gaagtgcgcg cgcggctcga gaagctgccg
    10141 gacatcctcg aggtgcgggt ggcgaacttc ggagaatgac ggcggaagac ggtggaggac
    10201 ggtgaaggtc gcaacggtgc aacctccgcc atcctccacc gacttccacc gccctccacc
    10261 gtcctcttct agttcacccg gtgtagctcc actctccggt tccgcgcacg gcccccggcc
    10321 gtcgcgttgg tgtctaccgg atcgtcctca ccgtaccagc gcgacaccac ctgctccggc
    10381 ggcacgcccc gttcgatgag atagccgcgg accgcgtcgg ccctggcctg cgacagccgc
    10441 aggttggcac cggccgtacc gcggctgtcg gtgtaaccgg cgacttccac gcgctgaccg
    10501 ggattcccct tgaggctcag cgcgacccgc tccaacgccc cccgtgcctg cgccgtcagc
    10561 acggtcttgc cggtctcgaa cgtcaccccc tcgagcacga gcgtccgctc cgcgccgacg
    10621 aacagggcgg ggcagccgat cgaatccacg cgctcgccgc ccggggtccc cgggcacttg
    10681 tcgttcgcgt cgacgatgcc gtcgcgatcc gcatcggtgg gcggtgggca accggtcgca
    10741 tccacgatcg cgcccgcggg cgtgttggcg cacttgtcca cggaatcgac gatgccgtcg
    10801 cggtcggagt cgcgggggca tcccgccgca tcgacggcct cgccgggcgc ggtgttcggg
    10861 caacggtccg catcgtccgc cacgtggtcg ccgtcggcgt ccttcggcag cgagcagccg
    10921 ctcgcgtcca cacgatcgcc ggccggcgtg tccgcgcacc ggtccaaccg gtccacgata
    10981 ccgtcggcgt cggagtcctt gggcagggca cagcccttgt cgtccacggc ttcacggggc
    11041 ggagtggcgg gacacgagtc cagccggtcc acgatgccgt ccccgtccga gtccttggga
    11101 tgggagcgcc ccatccacgt gctgagcccg gcctgcaccg tgaagttcca gttgttggga
    11161 acgccggcgg cctggttgtt gggcgacgga atgaaatcct cgatggcttc gatacgcatg
    11221 gcgaaggacg gccccatgcc gaagcgcagg ccgaacaggc cgctcacgcc gtcgtccgac
    11281 acgttgccgg tcgttccata gacgttgtgc acgtaaccgc cgcccaccag gaccgccacc
    11341 gcccccgcta tgggccgggc gtacaccagc cgggcgtgca atgggatcac ccggctgtcc
    11401 gcgacgaacg gtccggtcgt cggcaggtag ccgccggtcc cttcgagggc gaggccttgc
    11461 gccacgaata ctccgagccg gccgccgccg ccgaacgagt tatcgagtcc gagcgtgcgg
    11521 tcgaaccggg tgaagcggcc gaaggcaccg agctcgagtg tgccggggtc ctgagcggag
    11581 agtccggcgg atccgagtgc ggcgagaata acgagcgccg ccgggatgcg agagcgcatc
    11641 gcggcctcct ttcgcattgg ggtgagatga ctggcgggat taccccggag gcgcgggcag
    11701 gagtcgcggg aaacacgcgg gccaagagcg cgtgaggtgg gtcacattaa gaaaggttgt
    11761 ggaggacggt ggaggacgcg tcctccaccg tcttggctag ttgagccggt gcagctccac
    11821 ccgtcggttc cgcgcgcgcc cggtttccgt cgcgttcgag gcgaccgggc tggtggggcc
    11881 gtagccgcgc gccgtgagct gcagcggatc gacgccctgc gcgatcaggt aggcccggac
    11941 tgcgtcggct cgcgattggg ataggcgccg gttggtcgcc aggctgccgc ggctgtcggt
    12001 gtatcccgcg acctcgaccc gcagggtggg atacgcctgg agactggcgg cgacccgatc
    12061 gagggtgttg tgggactggt cggtgagatt ggagctggcg gtctcgaaat tcaccccttc
    12121 cagcacgagc gtccgctccg ccccgacgaa caggatccga cagccggccg catccacccg
    12181 ctcccccggg ggagtcgacg ggcacttgtc cacggcatcg agcacgccgt cctggtcggc
    12241 gtccttgggc agcgagcacc ccaccgcgtc cacctggtct ccagggggag tcgtggggca
    12301 cttgtcggtc atgtccggaa cgccgtcgcg gtccgtgtcg agcgggcacc cggcggcgtc
    12361 cacctggacg gcagcgggcg tcgccggaca gcggtcgttg ccgtccacga caccgtcccc
    12421 gtcggcgtcc atgggcaagg tgcagccgtc ggtatccacg tgctcgccgg cgggagtgtc
    12481 gtgacagcgg tcgttcgcgt ccacgactcc gtcggcgtcg gcgtccttgg gcagggggca
    12541 gccccgtccg tctacggcct ccccgcgcgg cgtaccgggg caggcgtccg cgtcgtccac
    12601 gacgccgtcg tggtcgctgt cacggactcc ccccgcgacg cccccgagct ggacgccgag
    12661 gccggcttga atggagaagt cccagttgtc ctgcaccccg acggtcgtgt tgagcggtga
    12721 ggggaagtag tcggctactc cttccaatcg gccgaccagc cggttgccga ggtccacgcg
    12781 cacgccgagc aggccgctga tgccgtcctc ccacccgtgg agactcttgc ggtacgtctg
    12841 gtggacgtat cccgcaccga ccagcacggc ggcgctcgcc gccaccgggg ccgcatagag
    12901 cagacggagc tccagcggga ccagtgagac cccgtctcct ccggcggtcg tcgtggactg
    12961 cacgaaggcg gcggcttcga cgcccaggtt gcgcatgacg aacacatcga ggcgcccgcc
    13021 acctccgatc gtgttgtcga aggtgaggga ctggtcgaaa cgcgtgaacc gggcgaacgg
    13081 gccgagctcg atggttccgg ggagttgggc cgccgcggga gaggcggcga gggtcacgat
    13141 ggccgcgagg cgcctggccg ctctcacgtg gcgacttccg gagcgagcga gggccccggg
    13201 agcggttggt cgtcgggcgg cggagctgga gccgctggtg gcggggccgg ctgctccgcc
    13261 acgatccgga acgacttcac tgctccctgc agacggtcgg cggcttcggc gagctgattg
    13321 gcggaggagg cgatctcctc cgtggacgcg gattgctgct ggctcgaggc cgcgatttcc
    13381 tgagcggacg ccagcagtcc gtcggttccc atcgccactg cggcgagccg ggccgcgatc
    13441 tcgtccacga ggtggcgcac ttcgaccgcc gaggtggcga tctcctggct ccaggcgtcg
    13501 ttcgcttccg cttcgcccgc gaccagcgac agtccctgcg acgcggtctt ggcggtttcc
    13561 cgcacgaccg ctccggtctg cgccagacgc tgcagccggt cgcgcgtttc ctgcgcgcgg
    13621 gtgagtacgc tgcggacggt gtcggcggtc tcggtcgcgg ctgcggccgc gagcgaggcc
    13681 agcttgcgga cctcgtcggc caccaccgcg aatccgcggc cctgcggccc ggcccgcgcc
    13741 gcctcgatgg cggcgttgag cgccagcatg ttcgtctgcg tggccaccgc cttcgcctgg
    13801 cccacgaatt tctggatctg cgcggacgag gtcgcgaggg catcggcgtc gtcggcccag
    13861 cgctcgacct cttcggcgag gcgcgtcagc tgggtgatgc tctggtccag ctgctcgcgg
    13921 tggtgcctgg ccaggcccgc gagctcggcg ttgcggcgca cggaatcctc ggcgcccgcg
    13981 gcgagcgcgg tggcgatctg gaggatccgc ccggcgtcgt cggcggccgc gcgcacgagc
    14041 tgggcctgct cagccgcccg cttcgtgagg tcctgcgtgg tcgacgtcat ctcctcggtc
    14101 gaggcgctca tctgctgagt ggccgccgaa atctccgccg ccatcgcggc cgactcgtcc
    14161 gaggcggagc ggatggcacc caccagctgg cgtagcgccg ccaccatccg ctgcaccgac
    14221 gtcgtcagat cgccgatctc tccggcctcc gattgcctgg cggtgtcggt gacggacagg
    14281 tcccccccgg cgacgcgggc ggcgatctcg ccggccgccc gcacgggtgc ggcgatgcgc
    14341 tggttgagcc accgcccgat ccacaacatc gccgccacgg cgagcccgaa caggatgacc
    14401 gccgccacca gcacggttcg ctgcaccgcc cgggagaggg tgtacgccgt gacggtgggc
    14461 tgatggaaca gcacccagat ccggctctgg ttcgccggga actgcccgcg ggccaccgga
    14521 acggagacga cgagctccgc gccgcggctc gcctcgagca cggtccggcg gggctggtcc
    14581 gggttgaaga gcgcacggtc ccgaggcacg ccgcgcagct cggcctgctc cgggccgaag
    14641 aggaggttgc cccgctcgtc caccagctgc aggtacgcgc tgtcgccgag acccgacctc
    14701 cccagcagca ggccgaggcg atcgagctgg tagatggcct ccagtacgcc gaccgcctgc
    14761 gcgccctccg ccgggcggat ggcgacggcg tagcgcaccg tgacggactg ggaagcggag
    14821 tcgaccgccg gagtggtttc cgcggcgtcc tgctgcatcg cctgctgcca caacggatcg
    14881 ctggcgcggg agaaccggtc cggccggctg gacgccatca cggtgaagcc gtggcgctcg
    14941 gtcacggcga gatccgtgaa gtccgatccg tcgggatagc gcttgaagta attctcgagg
    15001 ttgcggtcgc cgccgatctg atgcgtggcg ccgaacaggc gctccagcgt ggccggctcg
    15061 ctctggtcca ggcggcgctg cgagaccgtt tgcccggcct ccagcgccgc gcgcacgacg
    15121 aacgggttgg tggcgaggcg gtcggcctcg cggtgccggt cgcggatgta ggtcgccacg
    15181 aggtcggcgg cccgactggc gacctccgtg ccgtgctggt cggagagccg ctcgagcgag
    15241 cggcgggcgc tgcgggcggc gagcagcccg agtcccaaca ggacgatgag cgcggccgcc
    15301 gccacgctca tgctgaaccg ttgcagcagg tcccggagag tggtggccat ggctcgtcgc
    15361 gcaaagtatg ccgggtttct cggaggagca agtctgtgta atttcagtga cttatgtcgc
    15421 acgtaccggt ctacgacccg caggcggtgg aagcgcgctg gcaacagcgc tgggtggagc
    15481 gccacaccaa cgagccggag ctcgaccggc ccgcacggcc gttctacaac ctgatgatgt
    15541 tcccctaccc gtcggccgaa gggctgcacg tggggaacat gttcgcgttc accggcgccg
    15601 acatcttcgg acgcttccag cggctgcagg ggtacgacgt gttcgagccg atcggcttcg
    15661 acgcgttcgg catccactcg gagaactacg cgctctcggt cgggacgaac ccggcacagc
    15721 tcatcccgcg caacatcgac accttccggc gccagctgcg ccgcttcggg gggatgttcg
    15781 actggcgcca cgagctctcg accaccgatc cggcgtacta ccagtggacg cagtggctct
    15841 tcctgcagct cttcaaggcg aacctcgctt acaagaagcg cgcggcggtg aactggtgcc
    15901 cgaaggacaa gaccgtgctg gccaacgaac aggtcatcaa cggtcgctgc gagcgctgcg
    15961 atacgccggt ggagcaacgc tcgctggacc agtggttctt ccgcatcacc gactacgccg
    16021 agcggttgct gcgcaacctg gacacgctcg attggtcgga atccacactc gccctgcagc
    16081 gcaactggat cggccggagc gagggggcgg agctcgtgtt cacgaccccc gccggccaga
    16141 cgatcacggt cttcaccacc cgccccgaca ccgtcttcgg cgccacctac ctggtgctcg
    16201 cgccggagca cccgctggtg gaagcgctca ccgccgggga gcagcgcacg gccgtgcgcg
    16261 cctaccagcg ggaggtgcaa tcacgggacg tggtggcgcg ccgcgtcggc gagaaagtga
    16321 agaccggggt gttcctcggc tcctacgccc gcaatccggc cacgggcgag gccattccca
    16381 tctggatcgc cgactacgtc ctgatggagt acggcacggg cgcgatcatg gccgtgccgg
    16441 cccacgatga gcgggatcac gcgttcgcgt cgcaattcgg gctgcccatc cggcaggtgg
    16501 tgaacgtcgc gggagcagcg agcgagctgc cgagcgtttc cgaggacggc gtgctcatga
    16561 actcggggcg cttcgacggc ctggcgtgcc gggagggcgg gaaacagatc gtcgcctggc
    16621 tggcggagca gcaggtgggc tcgcccagag tccagtaccg gctccacgac tggtgtatct
    16681 cgcgccagcg ctactggggc ccgccgatcc cgatcatcta ttgcgatcgc tgcggaccgg
    16741 tccccgtgcc cgagcgggac ctcccggtgc tgctgccgct gatcgaggac ttccgccccg
    16801 acgacaccgg agtgtcgccg ctcgcccgcg tcgagtcgtg gtaccaggtg gcgtgcccgc
    16861 agtgcgggaa gccggcccgc cgcgagaccg acgtctccga caccttc
//
LOCUS       DBNGZW010000685         1323 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_675290, whole genome shotgun sequence.
ACCESSION   DBNGZW010000685 DBNGZW010000000
VERSION     DBNGZW010000685.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_675290"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..497
                     /locus_tag="AC16JC_16210"
     CDS             <1..497
                     /locus_tag="AC16JC_16210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166295.1"
                     /translation="TTRRAMIVRGLWAVAGAVGLGVAGTGANFAATKPVAPPAGPARL
                     SLVVRDVRFSAPASKPGTLPESDAVASPHGALQDATGRHLGRFSGGTLPGSGGQIAVQ
                     RFTFSDGTIIGMGSGRLDGDEYAVVGGTGRYAGATGTYVAQLRPGARGRDAEFQISVT
                     GTRG"
     gene            502..957
                     /locus_tag="AC16JC_16215"
     CDS             502..957
                     /locus_tag="AC16JC_16215"
                     /inference="COORDINATES: protein motif:HMM:NF017454.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HIR5166296.1"
                     /translation="MATDMFLKIDGIPGESTDAAHPGEIAVMSYSFGVSTSGGSAGSP
                     NFQDFHFVSQLQKSSPKLFLACASGQHIPSAILSVRRTNNGFPDFYKVILSNVIVTSF
                     EEGGTADSLETVPLEQISLNFAKIEIDYSVQDAKGVLGETVQVIWELGI"
BASE COUNT          204 a          462 c          432 g          225 t
ORIGIN      
        1 gcacgacgag acgcgccatg atcgtgcgcg ggctatgggc ggtcgcgggt gcggtcgggc
       61 tcggtgtcgc gggcacgggc gccaacttcg cggcgacgaa gcctgtcgca ccgccagccg
      121 gcccggcccg gctctcgctg gtcgtgcgcg acgtgcgctt cagtgcgccg gcctcgaagc
      181 ccggcactct cccggagtcc gacgccgtcg cctcaccgca cggcgccctc caggacgcga
      241 ccggacggca cctcggccgg ttctcgggcg gcaccctgcc gggttcgggc ggccagatcg
      301 ccgtccagcg gttcaccttc tccgacggaa cgatcatcgg catgggttct ggtcgcctcg
      361 acggcgacga gtacgccgtc gtgggtggca ccggccgata tgcgggcgcg accggcacgt
      421 acgtcgcgca acttcggcct ggcgcccgcg gccgcgatgc cgagttccag atcagcgtca
      481 ccggaaccag ggggtaaggc catggcgacc gacatgttcc tcaagatcga cgggattccc
      541 ggagaaagca cggacgccgc ccacccgggc gagatcgcgg tgatgtccta cagctttggc
      601 gtgtccacca gcggcggctc ggcgggctcg cccaacttcc aggacttcca tttcgtgtct
      661 caactgcaga agtcctcgcc caagctgttc ctcgcctgtg cgtcgggcca gcacatcccg
      721 tcggcgatct tgtcagtgcg taggaccaac aatggtttcc ctgatttcta caaggtgatc
      781 ttgagcaacg tcatcgtcac ctccttcgag gagggcggca cggccgactc cctggagact
      841 gttccgctcg agcagatcag cctcaacttc gcgaagatcg agatcgatta ctcggtccag
      901 gatgcgaagg gcgtgctcgg ggagacagtc caggtcatct gggaactcgg aatctgaact
      961 cgtttctcac tgccctcacg cgtcgccgga caccgcctga gcacttgctc tagctctgct
     1021 acgcgcggcg gccggccccg gcttgcggcc tcctcttcgg cgctctggta gaacgcctgc
     1081 cgcagcccgc gcggcacctg tcggaccgcg tgatgccaat gtgctggcca tatcgcggcc
     1141 tcggcgcgca tgtctgcatg gatcgggagc acgaccgatg caaccatcgg ccgcgccgac
     1201 cacaaggggt tgatgggagg tcgtcctcag ggacgaccga tgtcatcggc aggctcgacg
     1261 ctgaccgatt cgtcatggcg ctggtatcgc ccagcaccgg cgcggccgac gccgctcgcc
     1321 aca
//
LOCUS       DBNGZW010000686         2323 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1633841, whole genome shotgun sequence.
ACCESSION   DBNGZW010000686 DBNGZW010000000
VERSION     DBNGZW010000686.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1633841"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="AC16JC_16220"
     CDS             <1..858
                     /locus_tag="AC16JC_16220"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF017932.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M90 family metallopeptidase"
                     /protein_id="HIR5166297.1"
                     /translation="AHRRDSLRVVEALTIPGLVAALLLACLVGGWALVRRWRPRPRMR
                     VHRAAPGQWRALIEERVPLVRRLTEPEWGRLLDLVQRFLREKSFEGAGGIEVTEEMQL
                     VVAAQACLLILNLEAGCYPGVRSVILYPGTFVPRRPAIDGRMYVGVIPHPELLLGEHT
                     SGAVVLAWDSALHGAANSGDGSNVVLHEFAHELDREDGQVDGVPLLEAPSTTRAWSRV
                     LRERFEELRRTAEEGQPDVLNVYGATNRAEFFAVATEAFFSKPVEVRSRYPDLYDELK
                     GFYRQDPAA"
     gene            complement(824..1975)
                     /locus_tag="AC16JC_16225"
     CDS             complement(824..1975)
                     /locus_tag="AC16JC_16225"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011432894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR5166298.1"
                     /translation="MTPFLKTVESYRPGARTLPGRYYTAAEILAEEQETIFARHWISI
                     GRAADFPGHGDYRLVDIAGESLIVLREQGDTLHAFFNICRHRGTRLCTAPAGRLSETI
                     QCPYHAWTYALDGRLIGAPHMNEVEGFDKRDYPLHSAHLAEWEGFVFVSLAPDPPPFA
                     ASFAPLGERFQRFQLPRLRTVRRIVYEVRANWKLIMQNYSECLHCPTIHPELSTKLPY
                     TSGANDLVEGRFLGGYMEIKAPNQSATLTGRACALPLGDLSDEDRGRAFYYTLFPSMM
                     LSLHPDYAVYYTVWPAGPAESRVVCEWMVHPDAPGAPGYNIADAEEFWDRTNRQDWDI
                     CERSQLGVASRRYAPGPYSPRESVPAAWDREYLRQLGHLTQQDPGDRTP"
     gene            complement(1972..>2323)
                     /locus_tag="AC16JC_16230"
     CDS             complement(1972..>2323)
                     /locus_tag="AC16JC_16230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166299.1"
                     /translation="LRDGLWDVATREQLADTVVTALARHATNLRSAVLDRQVFAPPDL
                     EERFGVTEGHPYHGEMALDQIFFMRPVAGWARYRTPIKGLFLCGAGTHPGGGLPGAPG
                     RNAAREMLLQERRR"
BASE COUNT          338 a          755 c          858 g          372 t
ORIGIN      
        1 gcccatcgac gcgattcgct acgagtagtg gaagctctga cgattcccgg cctggtcgcc
       61 gcgctcctgc tggcgtgcct cgtcggcggc tgggcactgg tgcggcggtg gcggccccgg
      121 ccgcgcatgc gggtccaccg cgccgccccg ggccagtggc gagcgctcat cgaggagcgg
      181 gtgcccctcg tacgccgtct gacggagccc gagtggggcc ggctgctcga cctggtgcag
      241 cggttcctgc gcgagaagag cttcgagggc gccggcggta tcgaggtgac ggaggagatg
      301 cagctcgtcg tcgccgcgca ggcctgcctg ctgattctga acctcgaggc cgggtgctat
      361 ccgggagtgc gcagcgtgat cctgtacccc ggcaccttcg tcccgcgtcg tccggcgatc
      421 gacgggagga tgtacgtcgg ggtgatcccg caccccgagc tgctgctggg cgagcacacg
      481 agcggggcag tggtgctggc gtgggacagc gcgctccacg gtgcggccaa ctccggggac
      541 gggtcgaacg tcgtgctgca cgagttcgcc cacgagctgg atcgcgagga cggccaggtg
      601 gacggtgtgc cgttgctcga ggctccctcg acgacgcgcg cctggtcccg cgtgctgcgg
      661 gagcggttcg aggagctgcg gcgcacggcg gaggaaggac agccggacgt gctgaacgtg
      721 tacggcgcga ccaaccgggc cgagttcttc gcggtcgcca cggaggcctt tttcagcaag
      781 cccgtcgagg tgcgcagccg ctacccggat ctatacgacg agctcaaggg gttctatcgc
      841 caggatcctg ctgcgtgagg tgcccgagct gtcggaggta ctcccggtcc cacgccgccg
      901 gtacgctctc gcgcggcgaa tagggcccgg gggcgtagcg acgcgacgca acgccgagct
      961 gactgcgctc acagatgtcc cagtcctgac gattggtgcg gtcccagaac tcctcggcgt
     1021 ccgcgatgtt gtacccgggg gccccgggag catcggggtg caccatccac tcacacacca
     1081 cccggctttc ggccgggccc gccggccaga ccgtgtagta gaccgcgtag tcggggtgca
     1141 ggctgagcat catgctgggg aacagcgtgt agtagaacgc ccgcccgcgg tcttcgtcgg
     1201 aaagatcccc gaggggcagc gcgcacgcgc gtcccgtcag ggtggcgctc tggttgggag
     1261 ccttgatttc catgtacccg ccgagaaacc gtccttcgac gagatcgttg gcgccgctcg
     1321 tatacggcag cttggtgctg agctccgggt ggatggtggg gcagtggagg cactccgaat
     1381 agttctgcat gatcagcttc cagttggcgc gcacctcgta gacaatgcgc cgcaccgtcc
     1441 gcagccgggg aagctggaat cgttggaagc gctcccccag cggcgcgaac gacgctgcga
     1501 acggcggcgg atcgggcgcg aggctcacga acacgaaccc ttcccactcc gcgaggtgcg
     1561 cgctgtgcag cgggtagtcg cgcttgtcga acccttccac ctcgttcatg tgcggcgcgc
     1621 cgatcaatcg gccgtccagc gcataggtcc aggcgtggta ggggcactgg atggtctcgg
     1681 acaggcgtcc cgcgggcgcg gtgcagagcc gggtcccgcg gtgacggcag atgttgaaga
     1741 aggcgtggag cgtgtcgccc tgttcgcgca gcacgatgag ggactcgccg gcgatgtcca
     1801 ccagccggta gtcgccgtgc ccgggaaagt cggcggcccg gccgatggag atccagtgac
     1861 gcgcgaagat cgtctcctgc tcctcggcga ggatttcggc cgccgtgtag tagcgcccgg
     1921 gcagcgtgcg ggcgccggga cggtaggatt cgacggtctt gaggaacggt gtcatcggcg
     1981 gcgctcctgc agcagcattt cccgtgcggc gttccgtccc ggcgcgccgg ggagaccgcc
     2041 tcccgggtgc gtcccggcgc cgcacaggaa cagccccttg atcggcgtgc ggtagcgggc
     2101 ccagccggcc acggggcgca tgaagaagat ctggtcgagc gccatctcgc cgtggtaggg
     2161 gtggccctcc gtgacaccga agcgctcttc cagatccggt ggggcgaaga cctgacggtc
     2221 gagtaccgca ctccggagat tggtcgcgtg ccgggcgagc gccgttacga ccgtgtcggc
     2281 cagttgctcg cgcgtggcga cgtcccacag gccgtcgcgc agg
//
LOCUS       DBNGZW010000687         2520 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_63736, whole genome shotgun sequence.
ACCESSION   DBNGZW010000687 DBNGZW010000000
VERSION     DBNGZW010000687.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_63736"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..806
                     /locus_tag="AC16JC_16235"
     CDS             111..806
                     /locus_tag="AC16JC_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013164797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5166300.1"
                     /translation="MDFLTEGLRRALHLLATGDAEVLGITLLTLKIALLATAIACILG
                     IPAGFLLATRAFWGRRAALTVVNTALAFPTVVVGLLLFGLLSRRGPLGGLGLLYTWQA
                     ILIGDVVLALPVAAALSAAAVQGVDPRIRRTAETLGAGMWRSAWTVAREARFGLATVL
                     TAAFGQCTAEVGAALIVGGNIRGQTRTLTTAVALYTGQGDFGLALALGCILLLLALLV
                     NVALQVLQGHGRA"
     gene            799..1800
                     /locus_tag="AC16JC_16240"
     CDS             799..1800
                     /locus_tag="AC16JC_16240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR5166301.1"
                     /translation="MLELRGVRQRYEGRTVLELDHFAVERGTIVAIVGPNGSGKSTLL
                     RLAALLEAPTEGEVWLDNHPAPRTAGARRRVTLVEQRPALFRGTVRQNLGFGLRARGK
                     RRAEIDATVEAVSQRFGIADLLGRSRRELSEGEVQRVAVARAFALEPDLLLLDEPVSS
                     ADRAAALQLYRALQEERRRRDGGLTVCLASHQLEDAYRWSDDLRALADGRVVPVTPEN
                     LFRVVLHPGAPEVTRHVRVGTVDIAVATDRVGPAILAVPPSDIILSREPVASSARNVF
                     AGHVTRLARPRPGLVHVTADVGVELVAEVTEEAVRELGLLPGAPVTCAFKASAVRVF"
     gene            1803..2222
                     /locus_tag="AC16JC_16245"
     CDS             1803..2222
                     /locus_tag="AC16JC_16245"
                     /EC_number="4.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl trahydropterin synthase family
                     protein"
                     /protein_id="HIR5166302.1"
                     /translation="MPIVYLTRIVEFTATHRVARADWTPERNAATFGKAATDHAHRYQ
                     CRVTVKGPLVAGEGGVVNLTELDALLAREITDRLADGDINRDLPEFRAGKRLATGEAL
                     SVYLWERLAGVLPPGVRLHAIRVQEGPHLYSEYFGEP"
     gene            2230..>2520
                     /locus_tag="AC16JC_16250"
     CDS             2230..>2520
                     /locus_tag="AC16JC_16250"
                     /inference="COORDINATES: protein motif:HMM:NF014671.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HIR5166303.1"
                     /translation="MPAPAPAPAPGSRRGRMTADSALVEEWPVWLEVNGEPVVTWMCT
                     PDQLEELAAGWLHGEGYIESVDDLLKLRPCATDLGFWADVRPEGVARVKAENR"
BASE COUNT          321 a          898 c          913 g          388 t
ORIGIN      
        1 atcgtctact accggctgca gtacccggcg atcttccgcc ttgcccttct cccccccaac
       61 gcgtcgctca cggagtggac cgcccatccc tgggcgacat tcggcgtcgc gtggactttc
      121 tgaccgaagg cctgcgccgc gccctccacc tgctggcgac gggcgatgcc gaagtgctcg
      181 gcatcaccct cctcacgctg aagatcgcgc tgctcgcgac cgccatcgcg tgcatcctgg
      241 gtatccccgc cggcttcctg ctcgccaccc gggcgttctg gggacgccgc gccgcgctga
      301 cggtcgtcaa taccgcgctc gcgttcccca ccgtcgtcgt ggggctgctg ctcttcgggt
      361 tgctgtcgcg ccgcgggccg ctgggcgggc tcggactgct ttatacgtgg caggcgattc
      421 tgatcggcga cgtggtgctc gcgctgcccg tggcggcggc tctctcggcg gccgcggtcc
      481 agggtgtcga cccgcgcatc cgccgcaccg ccgaaacgct cggggcggga atgtggcgca
      541 gcgcctggac cgtggcgcgg gaagcgcgct tcggtctcgc caccgtcctc accgccgcgt
      601 tcggccagtg caccgccgag gtcggcgccg cgctgatcgt gggcggcaac atccgcgggc
      661 agacgcgcac gctcaccacc gcggtggcgc tgtacaccgg acagggggac ttcgggctcg
      721 ccctggcgct tggctgcatc ctgctgctgc tcgccttgct cgtgaacgtc gccctgcagg
      781 tcctccaagg gcacggccgt gcttgagctg cgtggggtgc gacaacgcta cgagggacgc
      841 accgtcctcg agctggacca tttcgccgtg gagcgcggca ccatcgtcgc catcgtcggc
      901 cccaacggct cggggaagag cacgctgctg cgcctcgccg cgctgctcga ggcgccgacg
      961 gaaggcgagg tctggctcga caaccacccg gccccacgga ccgcgggcgc acgccgccgc
     1021 gtgacgctgg tcgagcagcg cccggcgctg tttcgcggca cggttcgcca gaatctcgga
     1081 ttcgggttgc gggcgcgggg aaaacggcgc gccgagatcg acgccacggt cgaggcggtg
     1141 tcccagcgat tcggcatcgc cgacctgctg ggacgctcgc ggcgtgagct gtccgaaggg
     1201 gaggtgcagc gcgtggcggt ggcgcgcgcc ttcgcgctcg agccggacct gctgctgctc
     1261 gatgagcccg tgagctccgc cgaccgcgcg gccgcgctgc agctgtaccg ggcgctgcag
     1321 gaagagcgac gccgccgcga cggcgggctc accgtgtgcc tggcgtcgca ccagctcgag
     1381 gacgcgtacc gctggtccga tgatctgcgc gcgctggcgg acgggcgggt ggttccggtc
     1441 acgccggaga acctgttccg cgtggtgctc cacccgggcg cgcccgaagt gacccgtcac
     1501 gtccgcgtcg gcacggtcga catcgcggtg gcgaccgacc gcgtgggccc cgcgattctc
     1561 gcggtgccgc cgagcgacat catcctgtcg cgcgagccgg tggcgtcttc ggcgcgcaac
     1621 gtcttcgcag ggcacgtcac gcggctcgcc cgaccgcggc ccggactggt gcacgtcacg
     1681 gccgacgtgg gtgttgagct cgtggcggag gtcaccgagg aggcggtgcg cgagctggga
     1741 ctgcttcccg gggcgccagt cacctgcgcc ttcaaggcca gtgccgtgag ggtattctga
     1801 ccatgccgat cgtctatctt acgagaatcg tggagtttac ggcgacgcat cgggtcgccc
     1861 gggcggactg gaccccggag cgcaacgcgg ccaccttcgg caaggcggcc acggaccatg
     1921 cgcatcgcta tcagtgccgg gtcactgtga aggggccgct cgtcgcgggc gagggcggag
     1981 tggtcaacct caccgagctg gatgcgctcc tggcccgcga gatcaccgat cggctggcgg
     2041 acggcgacat caaccgggac ctgccggaat tccgggccgg caaacgtctg gcgaccggcg
     2101 aagccttgtc ggtgtacttg tgggagcggc tggccggcgt gctgccgccc ggcgtacggc
     2161 tccatgcgat ccgcgtccag gagggacccc atctctactc cgagtacttc ggcgagccct
     2221 gagcacgaag tgcccgcccc cgcccccgca ccagcccccg ggtcgcgccg cggccggatg
     2281 acggcggact ccgccctcgt ggaggagtgg ccggtgtggc tcgaggtgaa cggcgagccc
     2341 gtcgtgacgt ggatgtgcac gcccgaccag ctcgaggagc tggcggccgg ctggctgcac
     2401 ggcgagggct acatcgaatc ggtggacgat ctgctcaagc tgcggccctg cgccaccgac
     2461 ctgggcttct gggcggacgt gcggcccgag ggcgtggccc gcgtgaaggc ggagaaccgc
//
LOCUS       DBNGZW010000688         3119 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1291783, whole genome shotgun sequence.
ACCESSION   DBNGZW010000688 DBNGZW010000000
VERSION     DBNGZW010000688.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3119)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3119)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3119
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1291783"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..1962)
                     /locus_tag="AC16JC_16255"
     CDS             complement(160..1962)
                     /locus_tag="AC16JC_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR5166304.1"
                     /translation="MTTPANTPRTAATSPAQVGTYSPASQSTFRTCQVTGRRIHDRAE
                     NVIKANAVTAIVFLLVGTIAAVLLVLTRWQAVHLLSATWYYRLLGVHAMSMLIFFIIF
                     FEMAVLHFASTVLLNARNSMPKLAWTSYALMLGGALLVETMMWGGKADVLFTSYVPLR
                     ANPLYYLGVILFAVGALAVQWIFFRNLVVAKRERTYEGSVPLVVYGAATAAIIAVITL
                     LHGAAIYIPTFLWSLGLMKVDPQVYRMIWWGLGHSSQQINVAAMVAVWYMLGALTIGA
                     VVLNEKISRSAFVLYILFISMASAHHLLVDPGLGPAWKVVNTSYFMYMAVLASMIHGF
                     TVPAGMELGMRLRGYTNGMFEWLRKAPWGDPGFSSLVLSVVVFGFVGGITGVTIGTEQ
                     INIIVHNTLRVPGHFHATVVSGTAMAFMGATYYLIPLIFQKRVAFWKMAKMQPYFFAG
                     GMFIFTVSMTFAGNFGVPRRHWDITFSQAPYSVPFNPIVDLLLALVALGGLIAATGAF
                     MFVAIAVKSVFFGTPLGEFTRGVEAVGLPRGITHQPVHAADVDERNAELHAASRGIMG
                     PAPGTLVLVFVFLAVFALYFFSNWKILSFLWRIG"
     gene            complement(1959..2552)
                     /locus_tag="AC16JC_16260"
     CDS             complement(1959..2552)
                     /locus_tag="AC16JC_16260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008613537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit II"
                     /protein_id="HIR5166305.1"
                     /translation="MHTETHTGLDSPRGNWWKPAHKTEKVWVGIAFGWCMVLFAMMPL
                     WHWKGGQNPSGIRRRVEPTAFFARTEEFIKQYKVGDDRGMPIVQPPPGADVYLTAMAF
                     QWRPILRLQKGAQYTLHLSSLDVNHGFSLFPINVNFQVVPGYDYGLRVRPNTSGDFRI
                     MCNEFCGIGHHTMVGRVIVDDAPAVAVTAATTTGGAR"
     gene            complement(2560..2742)
                     /locus_tag="AC16JC_16265"
     CDS             complement(2560..2742)
                     /locus_tag="AC16JC_16265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166306.1"
                     /translation="MAETPPVDPTPPSAAEPVPRGQRFFDNIHLLLVLGVVIVFALYT
                     GWGMWEILTLPKATLP"
BASE COUNT          576 a         1092 c          984 g          467 t
ORIGIN      
        1 gcaatccggt ggccgcggtg ccgaagcacg tctcgcgcgt cagcaccagc cacgccggcg
       61 cgtcggcggt gggccacagc gcgagcgccc accagctggc ggtgatcacg acgatcaccg
      121 ccaacgccga cagcgccacc cacgcccggg acgccgccct catccgatcc tccagaggaa
      181 cgacaggatc ttccagttgc tgaagaagta cagcgcgaac acggcgagaa agacgaacac
      241 cagcacgagc gtgccgggcg ccggtcccat gatcccgcgc gaggccgcgt gcagctcggc
      301 gttgcgctcg tcgacgtcgg cggcgtgcac cggctggtgc gtgatcccgc gcggcagacc
      361 caccgcctcg acgccacgcg tgaactcgcc gagcggcgtg ccgaagaaca ccgacttcac
      421 cgcgatcgcg acgaacatga acgcgcccgt cgccgcgatc agcccgccga gcgccacgag
      481 cgcgaggagc aggtcgacga tcgggttgaa cggcaccgag tacggcgcct ggctgaacgt
      541 gatgtcccag tgccgccgcg gtacgccgaa gttccccgcg aacgtcatcg acacggtgaa
      601 gatgaacatc ccgccggcga agaagtacgg ctgcatcttg gccatcttcc agaacgcgac
      661 gcgcttctgg aagatcagcg ggatcaggta atacgtcgcg cccatgaacg ccatcgccgt
      721 cccgctcacc accgtcgcgt ggaagtggcc cggcacgcgc agcgtgttgt ggacgatgat
      781 gttgatctgc tccgtgccga tcgtcacgcc ggtgatcccg ccgacgaagc cgaacaccac
      841 caccgagagc acgagcgagc tgaagccggg atccccccac ggcgcctttc gcagccactc
      901 gaacatgccg ttcgtgtagc cgcggagccg catcccgagc tccatccccg ccggcacggt
      961 gaagccgtgg atcatcgacg cgagcactgc catgtacatg aagtagctcg tgttcaccac
     1021 cttccacgcc ggtccgaggc cggggtccac cagcaggtgg tgcgccgacg ccatcgagat
     1081 gaacaggatg tacagcacga acgccgagcg gctgatcttc tcgttcaaca cgacggcacc
     1141 gatcgtcagc gcgccgagca tgtaccacac cgccaccatc gccgcgacgt tgatctgctg
     1201 cgacgagtgg cccagccccc accagatcat gcggtagacc tgcgggtcca ccttcatcag
     1261 gccgagcgac cagaggaagg tcgggatgta gatcgccgcg ccgtgcagca gcgtgatcac
     1321 ggcgatgatc gccgccgtcg ccgcgccgta caccacgagc gggaccgagc cctcgtacgt
     1381 ccgctcgcgc ttggccacca cgaggttccg gaagaagatc cactgcacgg ccagcgcacc
     1441 caccgcgaac aggatcacgc cgaggtagta cagcgggttc gcgcggagcg gcacgtacga
     1501 ggtgaacagc acgtcggcct tcccgcccca catcatcgtc tccaccagca gcgcgccgcc
     1561 gagcatgagc gcgtacgacg tccacgccag cttcggcatc gagttgcggg cgttcagcag
     1621 cacggtgctc gcgaagtgca gcaccgccat ctcgaagaag atgatgaaga agatcagcat
     1681 gctcatcgcg tgcacgccga gcagccggta gtaccaggtg gcgctcagca gatgcaccgc
     1741 ctgccaccgg gtgagcacga gcagcacggc ggcgatcgtc ccgacgagca ggaacacgat
     1801 cgccgtcacg gcgttggcct tgatcacgtt ctccgcgcgg tcgtggatgc gccgccccgt
     1861 cacctggcag gtgcggaacg tcgactgcga ggcgggcgag tacgtgccga cctgcgccgg
     1921 cgacgtcgcc gcggtgcgcg gcgtgttcgc gggggtcgtc atcgcgcacc tcccgtcgtc
     1981 gtcgccgcgg tgaccgccac agcaggcgcg tcgtcgacga tcacgcggcc gaccatcgtg
     2041 tgatggccga tgccgcagaa ctcgttgcac atgatgcgga agtcgcccga cgtgttgggc
     2101 cgcacgcgca ggccgtagtc gtagcccggc accacctgga agttgacgtt gatcgggaac
     2161 agcgagaacc cgtggttcac gtcgagcgac gacaggtgca gcgtgtactg cgcgcccttc
     2221 tgcagtcgca ggatcggccg ccactggaac gccatcgccg tcagatacac gtcggcgccc
     2281 ggcggcggct gcacgatcgg catcccgcgg tcgtcgccca ccttgtactg cttgatgaac
     2341 tcctcggtgc gcgcgaagaa cgccgtcggc tccactcggc ggcggattcc cgacgggttc
     2401 tgaccgccct tccagtgcca gagcggcatc atcgcgaaca gcaccatgca ccagccgaag
     2461 gcgatgccga cccacacctt ctcggtcttg tgcgccggct tccaccagtt gccgcgaggg
     2521 gaatccagcc ccgtgtgcgt ctcggtatgc atggtcgcct cagggaaggg tggccttcgg
     2581 aagggtcagg atctcccaca tcccccaccc ggtgtagagc gcgaacacga tgaccacacc
     2641 gaggacgagc aggaggtgga tgttgtcgaa gaagcgctga ccgcgcggca cgggctcggc
     2701 cgcggagggg ggcgtcgggt cgactggtgg tgtttcggcc atggagcgct cctgtggcgg
     2761 aagctgagag gtcggggatt cgccgatgct acggctcccc gaaagtcccc gcagtccggg
     2821 atttctcacc tttgtatggg aatttccccg acggcagccc catcgccccg gtctacgttg
     2881 gttggggcgg tggtgggaga gtacggtgag cggatagggc agtgaatgga tcgggcggtg
     2941 aatggagtga atgggctgat cgccctctca ccgatacacc gattcaccga ttcacccatt
     3001 caccgattct cccattcacc gctccaccca ctcaccgctc cacccattca ccgctccacc
     3061 cactcaccgc tccacccact caccgctcca cccactcacc gcatctttca atacctcaa
//
LOCUS       DBNGZW010000689         3549 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_731250, whole genome shotgun sequence.
ACCESSION   DBNGZW010000689 DBNGZW010000000
VERSION     DBNGZW010000689.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3549)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3549)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3549
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_731250"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..245)
                     /locus_tag="AC16JC_16270"
     CDS             complement(<1..245)
                     /locus_tag="AC16JC_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR5166307.1"
                     /translation="MLTIAISQFRPAKGEYAANLERIGAVLGKAVSLDPRPALVVFPE
                     TATSGYFVEGGVKEVAVTAGTLLRDLDRVYQAPDRPPV"
     gene            346..882
                     /locus_tag="AC16JC_16275"
     CDS             346..882
                     /locus_tag="AC16JC_16275"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIR5166308.1"
                     /translation="MLHPWHDLPAGPHPPDEVTAIIEIPSGSRNKYELDKETGLFRLD
                     RVLYSAVHYPGDYGFIPRTLSEDNDPCDVLVLIKEPTFVGCEIRARPIGLLRMVDRGE
                     PDEKILAVPAQDPFQNEFFDIADIPQHQLKEIEHFFLIYKDLEGKRTQMEGWRKSDVA
                     MAAISAAMQRYAERYPGR"
     gene            879..1280
                     /locus_tag="AC16JC_16280"
     CDS             879..1280
                     /locus_tag="AC16JC_16280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166309.1"
                     /translation="MSVGLLTRNQERRLATHLRLLLDDLDALATLPELSRSGQAGRRV
                     LGLLDETRAAARELRSALGLPEEQGAHPARRVVATAEVWAVRVEDLRARRLGAYGEVH
                     PDLGATLDPRLDQLRRALVALAEAATGLPER"
     gene            1283..1657
                     /gene="crcB"
                     /locus_tag="AC16JC_16285"
     CDS             1283..1657
                     /gene="crcB"
                     /locus_tag="AC16JC_16285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIR5166310.1"
                     /translation="MLLYIAAGGLLGTLGRYFLQGWIQTRSGAGAFPLGTLLINLAGS
                     FVLGFVIRFATGSTVVSPEIRAGLTIGFCGAFTTMSTFSYEATRLLGDGEYWSAALYM
                     AGTIVGCLGAVIAGTALAERIL"
     gene            1746..2144
                     /locus_tag="AC16JC_16290"
     CDS             1746..2144
                     /locus_tag="AC16JC_16290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010941172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HIR5166311.1"
                     /translation="MMHHRFKGERTLMRIFIGESDKFHGKPLFQVLLERLREKGFAGA
                     TVLRGVAGFGASSTMHTDKVLRLSLDLPIIIEVVETEEAIQQVLPDLDEMIGGGLITL
                     ERARVIMYRPGGTRPSERELHRIEGLEPEG"
     gene            2163..2789
                     /locus_tag="AC16JC_16295"
     CDS             2163..2789
                     /locus_tag="AC16JC_16295"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR5166312.1"
                     /translation="MKAALGLLACLVALTAIVAAGWFAPLDQAAAVAAATHRTAFQSA
                     VAINVTALGSAPVIVIVAVVAAAYAVASGRPRLVLAIAWTPLAFLLDSAIKLLVRHPR
                     PTVAMIALPPDFSFPSGHAVAASALYVTLALLAAGVERRAGPRRLLIAAGVLVALLVA
                     WSRVYLGVHYLTDVVGGLLLGTAGAIVAVRATRDGDQVDRQALSHSDA"
     gene            complement(2845..>3549)
                     /locus_tag="AC16JC_16300"
     CDS             complement(2845..>3549)
                     /locus_tag="AC16JC_16300"
                     /inference="COORDINATES: protein motif:HMM:NF012544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5166313.1"
                     /translation="YLDAGKIAEEVVEPSRSLPRIYLGAIVAVTALYLLLNVAYLQVL
                     PFDRIASSNLVAADVMAVLFGGGAGVIFAGIALLVVLASLNGNIFVTPRVIFGLAREG
                     LAPGVLARVNRGGTPWVAMAFVGVTAMAMALGGTFTSLLALSITLILVVDSIAVLALL
                     VLRRRAPDAPFRTPLYPGLPLAFVGVYGALLVGTVIAQLKVVLVSVAVIAVAYLLAWL
                     TARDQTDRQSLSGSGA"
BASE COUNT          591 a         1237 c         1173 g          548 t
ORIGIN      
        1 acgggcgggc ggtccggcgc ctgatagacg cggtccaggt cgcgcagcag ggtccccgcc
       61 gtcaccgcga cctccttcac gccgccttcc acgaagtatc ccgacgtggc cgtttcgggg
      121 aacacgacca gtgccggccg gggatccaga gacacggcct tgccgagcac cgcgccgatc
      181 cgctcgagat tggcggcgta ctcgcccttc gcagggcgga attgggaaat ggcgatggtc
      241 agcattgctg gaaagatacg cgatcggcta tcggctatcg gctatcagct attcggcacg
      301 cgcgcgccga tagccgatag ccgatagcgc ctttaggttc tcagcatgct ccacccctgg
      361 cacgatctcc ccgccggtcc gcatccgccg gacgaagtca cggccatcat cgagatcccg
      421 agcggcagcc gcaacaagta cgaactggac aaggagaccg gcctgttccg gctcgaccgc
      481 gtcctctact cggcggtgca ctacccgggc gactacggct tcattccccg cacgctgtcc
      541 gaggacaatg acccgtgcga cgtgctggtg ctgatcaagg agcccacctt cgtaggttgt
      601 gagatccgcg cgcgccccat cggcctgttg cgcatggtgg accgcggaga gccggacgag
      661 aagatcctgg ccgtaccggc ccaggacccg ttccagaacg agttcttcga catcgccgac
      721 atcccgcagc accagctgaa ggagatcgag cacttctttc tcatctacaa ggacctcgag
      781 gggaaacgga cgcagatgga ggggtggcgc aagagcgacg tggcgatggc agcgatctcg
      841 gcagcgatgc agcgctacgc cgagcggtac cccgggcgat gagcgtgggc ctcctgaccc
      901 gtaaccagga gcggcgactc gctacgcacc tgcgtctgtt gctggacgac ctcgacgcgt
      961 tggccacgct cccggagctg agccgctccg gacaagccgg gcgacgcgtg ctcgggttgc
     1021 tcgacgagac gcgggccgcc gcgcgcgagc tacgtagcgc gttgggcctc cccgaggagc
     1081 agggcgccca cccggcgcgc cgtgtcgtcg cgacggccga ggtatgggcg gtgcgcgtgg
     1141 aagacctgcg cgcccgccgt ctgggggcgt atggcgaggt ccatcccgat ctcggcgcga
     1201 cgctcgaccc gcggctcgac cagctgcgcc gcgccctggt ggcgctggcc gaggcggcga
     1261 ccggacttcc cgagcggtga cgatgctcct ctacatcgcg gcaggcggcc tgctcggcac
     1321 actcggccgc tacttcctgc aagggtggat ccagacgcgg tccggcgcgg gtgcgtttcc
     1381 actgggcacc ctgctcatca acctcgccgg ctcgttcgtc ctcggcttcg tgatccgctt
     1441 cgccaccgga tccaccgtcg tgtcccccga gatccgggcc ggactcacga tcgggttctg
     1501 cggtgccttc accaccatga gcaccttcag ctacgaggcc acgcgcctcc tgggcgacgg
     1561 cgagtactgg tcggccgcgc tgtacatggc cgggacgatc gtgggatgcc tgggagcggt
     1621 gatcgcgggg accgcgctgg cggagagaat actatgacgg ggtgtcaggt gccgggtgtc
     1681 aggtgccgga agcgccaagc cgtccccgaa acccgacacc aggcacctga cacccggaga
     1741 ttctcatgat gcaccatcgg ttcaagggcg agcgcaccct gatgcgcatc ttcatcgggg
     1801 aaagcgacaa gttccacggc aaaccgctct tccaggtgct gctcgagcgg ctgcgcgaga
     1861 aggggttcgc gggagccacg gtgctgcgcg gcgtggccgg ctttggcgcg tcgagcacga
     1921 tgcacaccga caaggtgctc cgcctctcgc tcgatctccc gatcatcatc gaggtggtgg
     1981 aaaccgagga ggccatccag caggtgttgc cggatctgga cgagatgatc ggaggaggcc
     2041 tgatcaccct cgagcgcgcg cgcgtgatca tgtaccgccc cggcggcact cggccgagcg
     2101 aacgcgagct gcaccgcatc gagggactcg agccggaagg ctaggaggct agattgagcg
     2161 gtgtgaaagc ggccctcggg ctcctggcct gcctcgtcgc cctcaccgcg atcgtcgcgg
     2221 cggggtggtt cgcgccgctc gatcaggctg cggcggtcgc cgccgccacc caccgtacgg
     2281 cgttccagag tgccgtggcg atcaacgtga cggccctggg tagcgcaccc gtgatcgtca
     2341 tcgtggccgt tgtcgcggcg gcctatgccg tcgcatccgg ccggccgcgt ctggtattgg
     2401 cgatcgcctg gacccccctc gccttcctgt tggacagcgc catcaagctg ctggtgcgcc
     2461 acccccgtcc caccgtcgcg atgatcgcgc tgccgccgga cttcagcttt ccgagcggtc
     2521 acgccgtcgc cgccagcgcg ctgtacgtca cgctcgccct ccttgccgcc ggcgtggagc
     2581 ggcgcgccgg cccgcgccgc ctgctcatcg ctgccggcgt cctcgtggcg ctgttggtgg
     2641 cctggagcag ggtgtacctc ggcgtgcact acctcacgga cgtggtcgga gggctgctac
     2701 tggggacggc gggagcgatc gtagcggtaa gggcgacaag agacggtgat caggtggaca
     2761 gacaggcgct gagtcactca gacgcgtagc ggttgtcagt tgccagctgt caggtctttc
     2821 ctcccaccta tcgcctgcca accgctacgc gcctgagcca ctgagcgact gtctgtccgt
     2881 ctgatctctg gccgtcagcc atgcgagcag gtatgccact gcgatcaccg ccaccgacac
     2941 cagcaccact ttcagttgcg cgatcaccgt tcccacaagc agggcgccgt acacccccac
     3001 gaaggccagc ggcagcccgg gatacaacgg ggtccgaaac ggagcgtccg gcgcgcgccg
     3061 ccgcagcacc agcaatgcca gcaccgcgat cgagtccacg accaggatca gcgtgatcga
     3121 gagcgcgagc agcgaggtga aggtcccccc gagcgccatc gccatcgccg tcacaccgac
     3181 gaacgccatc gctacccacg gcgtgccacc gcgattcacc cgcgccagta caccgggggc
     3241 cagaccctcg cgtgccaggc cgaagatcac ccgcggcgtc acgaagatgt tgccgttgag
     3301 cgaggcgagc acgacgagca gagcgatgcc cgcgaatatc acccccgccc cacccccgaa
     3361 gagcaccgcc atcacatccg ctgccacgag gttcgaggac gcaatgcggt cgaagggcag
     3421 cacctgcagg taggccacgt tgagcaacag gtagagcgcg gtgacggcca cgatcgcacc
     3481 caggtagatc cgcggcaggc tgcgcgacgg ctcgaccact tcctcggcga tcttccccgc
     3541 gtccagata
//
LOCUS       DBNGZW010000690         3454 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1360407, whole genome shotgun sequence.
ACCESSION   DBNGZW010000690 DBNGZW010000000
VERSION     DBNGZW010000690.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3454)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3454)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3454
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1360407"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="AC16JC_16305"
     CDS             <1..506
                     /locus_tag="AC16JC_16305"
                     /inference="COORDINATES: protein motif:HMM:NF037222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166314.1"
                     /translation="SSARVFVLPEPTGVPGGAAYFADLRLTSADGRPLSTNLYWLSTR
                     PDVLADTSTWYMTPVKSYADFTALRSMPAAVVDATARFSARAGWGEARVTLSNPGRSI
                     AFFMRLQVTGRGGQEALPVLWEDNYLSLLPGETRMVTATYRVRDLGGAPPRVVVSGWN
                     VRRTIAR"
     gene            503..973
                     /locus_tag="AC16JC_16310"
     CDS             503..973
                     /locus_tag="AC16JC_16310"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016107.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase family protein"
                     /protein_id="HIR5166315.1"
                     /translation="MKHPLRLLMRIPVPWVFVLAYLVGAGLEQVWPLELHRAAPRGIG
                     IAGGVLFAIGAGIAGWAWAIFHRAGTTTVPGQPSVSLITRGPYRASRNPMYVGLALAY
                     LGEAGMLRQVWPVLVLPLTLAYLHWMVIPVEEARLREVFHGEYEEYCTSVRRWI"
     gene            1005..1559
                     /locus_tag="AC16JC_16315"
     CDS             1005..1559
                     /locus_tag="AC16JC_16315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166316.1"
                     /translation="MSMSAERTRAVLVAYQQVTAASWPDLHPEMAARSLAWSSADADA
                     LERELQGYVRATSAPDCPVRVFWKLLPGFTFGGCNDHFARDAGMSSAELLGHDDFDRR
                     LPWVFQAAKYRADDEAVVQSPHPKLNIVERQRSSTGVITWVRVGKAAIRTAAGVIGVL
                     GMYEVLDAETGRRAFAEQARRHAR"
     gene            1606..>3454
                     /locus_tag="AC16JC_16320"
     CDS             1606..>3454
                     /locus_tag="AC16JC_16320"
                     /inference="COORDINATES: protein motif:HMM:NF012931.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HIR5166317.1"
                     /translation="MRLLPAFPALLLALTTRAAAAQSALQLRWELKEDVFQGAQGLSR
                     AAFTLTNRDAKPLAPRGWAIYYSAIHAPQPGSVGSGFTIEDVAGDLHRIVPGAGFAGL
                     APGASVRIEYLTDLLLNVSFAPKGPYIVFDDAKDVGVPLNDYVAVPFERPAQGEGRDP
                     RVVSPETQFTLDSVIRDIPASELPPVFPTPVQVTMGAGALRLSAMPQVAATSGLEQEA
                     TFAAEYLRPYFRAGRKGGVPPLRLEVGLVEGQTSPEAYELVVDPTAGVRIVGVSSAGV
                     FYGLQSLRSLLPPPSPRGELLVPALRVVDAPRFGYRGFMLDVARNFQPKPAVLRTLDL
                     LARYKLNVFHLHLTEDEAWRIEIPSLPELTAVGARRGHTLDSGTNLPPAYGSGPTVDR
                     PWGSGFFSRADYIEILRYAAARHIEVIPELEMPGHARAAIKAMEARSRTTGDRQYLLS
                     DPEDRSVYTSAQGYHDNAMNPVLESTFGFIERVVGDLVAMHREAGVPLRHIHMGGDEV
                     PAGVWEGSPAVQAYLKAHNLTSVDDLWFVFYGRVEQILKAQGLLPSGWEEIAVRKTHR
                     DGRPTNIPNPDFAARGWRAYVWDNVPGGGAEDLAYRLANGGYDVVLSPVS"
BASE COUNT          500 a         1187 c         1197 g          570 t
ORIGIN      
        1 acagcagtgc gcgcgtgttc gtgctgccgg agccgacggg tgtgccgggc ggagcggcgt
       61 atttcgcaga cctccgcctg acgagcgccg acggacgccc tctcagcacc aacctgtact
      121 ggctctccac ccgtcccgac gtattggccg atacctcgac gtggtacatg acgccggtga
      181 agtcgtacgc cgatttcacc gcgctcagaa gcatgcccgc ggctgtggtg gacgcgaccg
      241 cgcgcttcag cgctcgcgcc ggctggggcg aggcacgggt gacgctgagc aatcccggcc
      301 ggtccatcgc cttcttcatg cgcctgcagg tgaccgggcg cggcggccag gaagcgttgc
      361 ccgtgctctg ggaggacaac tacctgtcgt tgctgccggg ggagacgcgc atggtcacgg
      421 ccacctaccg cgtacgcgac cttggcggcg cgccgccgcg cgtggtcgtg agcgggtgga
      481 acgtgcgccg gaccattgcc cgttgaagca ccccctccgg ctgctgatgc ggatccccgt
      541 tccctgggtg ttcgtgttgg cgtacctcgt gggcgccggt ctcgagcagg tgtggccgct
      601 cgagctccat cgggcggcac cgcgcggcat cggcatcgcg ggcggcgtgc tgttcgcgat
      661 cggagcggga atcgccgggt gggcctgggc catattccat cgcgcaggca cgaccacggt
      721 tccggggcag ccatccgtca gtctcatcac aaggggaccc taccgcgcga gccggaaccc
      781 gatgtacgtg ggcctcgcgc tcgcttacct gggtgaagcg ggcatgctcc ggcaggtctg
      841 gcccgtcctg gtgctgccgc tgacgctggc ctatctgcac tggatggtca tcccggtcga
      901 agaggcgcgc ctgcgggagg tctttcatgg tgagtacgag gagtactgca cgagcgtccg
      961 tcggtggatc tagctgatcc cgcaagcggg gagggctcga ggccatgagc atgagtgctg
     1021 agcgcacgcg ggccgtactg gtcgcgtacc agcaggtgac ggccgcgtcg tggcccgacc
     1081 tgcatccgga gatggcggcg cgatccttgg cctggtcgtc cgccgatgcg gacgcgctcg
     1141 agcgcgagct ccagggctac gttcgggcga catcggctcc cgactgcccc gtccgcgtgt
     1201 tctggaagct gctgcccggc ttcaccttcg gtggctgcaa cgaccatttc gcgcgcgatg
     1261 cgggcatgtc gagcgcggaa ctgctcggcc acgacgattt cgatcgacga ttgccgtggg
     1321 tgttccaggc cgcgaagtac cgtgccgacg acgaggcggt cgtgcagtct ccccatccga
     1381 aattgaacat cgtggaacgc cagcgaagct cgacgggagt catcacctgg gttcgggtgg
     1441 gcaaggcggc gatccggacc gccgccgggg tgatcggggt cctggggatg tacgaggtgc
     1501 tcgacgctga gactggacgc cgggcgttcg ccgagcaggc ccgtcgccat gcacgatgat
     1561 cttcctcgcc tggacaccgg ggcccaccgg ggtctaatgt ccctcatgcg acttcttcct
     1621 gcgtttcccg ctctcctgct ggccctgacg accagggcgg ccgccgccca gtccgctcta
     1681 cagctccgct gggagctcaa agaggacgtc ttccagggcg ctcaaggcct gtcgcgcgcg
     1741 gccttcaccc tcaccaaccg cgacgcaaag ccgctcgccc cgcgcggatg ggcgatctac
     1801 tacagcgcga ttcacgcgcc gcagccggga agcgtgggga gcgggttcac gatcgaggac
     1861 gtggcgggcg acctgcaccg catcgtgccc ggcgccggct tcgccggact ggcccccggc
     1921 gccagcgtcc ggatcgaata cctcacggac ctgctcctca acgtcagctt cgctccgaag
     1981 ggtccgtaca tcgtgttcga cgacgcgaag gacgtcggcg ttcccctcaa cgattacgtg
     2041 gccgtcccct tcgagcgccc cgcgcagggc gaagggcgcg atccccgcgt ggtgagcccc
     2101 gagacccagt tcacgctcga ctccgtgatc cgcgacatcc ccgcgagcga gctgccgccg
     2161 gtgttcccga cgccggtgca ggtgacgatg ggagcgggag cgttgcgcct cagcgcgatg
     2221 ccgcaggtcg ccgcgacgtc cgggctcgag caggaggcga cattcgccgc cgaatacctg
     2281 cggccctact tccgcgcggg tcggaaggga ggagtcccgc cgctgcgact cgaggtgggc
     2341 ctggttgagg gccagacgtc gcccgaggcg tacgagttgg tggtcgatcc cacggccggg
     2401 gtccggattg tcggcgtttc gtccgccggc gtcttctacg ggctccagtc cctgcgcagc
     2461 ctgctcccgc cgccctcgcc tcgcggagag ctcctcgtgc cggcgctgcg ggtggtggac
     2521 gccccgcgct tcggctaccg cgggttcatg ctcgacgtcg cgcgcaactt ccagccgaaa
     2581 ccggcggtgc tgcgcacgct cgacctgctc gctcgctaca agctcaacgt cttccacctg
     2641 caccttaccg aggacgaggc ctggcgcatc gaaatcccga gccttccgga gctgaccgcg
     2701 gtgggcgcac ggcgcggcca caccctcgac tcagggacga atcttccgcc cgcctatggc
     2761 tcgggcccca ccgtggaccg gccgtggggc agcggcttct tctcccgcgc ggactacatc
     2821 gagatcttgc gctacgcggc ggcgcggcac atcgaggtga ttcccgagct cgagatgccg
     2881 ggccacgcgc gcgccgcgat caaggccatg gaggcccgat ctcgcacgac cggagaccgg
     2941 cagtacctgc tgagcgaccc ggaggaccgc tccgtgtaca cgtcggccca ggggtaccac
     3001 gacaacgcga tgaacccggt actcgagtcg accttcgggt tcatcgaacg ggtggtcggc
     3061 gacctcgtgg cgatgcaccg cgaggcgggc gtgcccttgc gccatatcca catgggagga
     3121 gacgaggtgc cggcgggggt ctgggaaggc tcgccggccg tccaggccta tttgaaggcg
     3181 cacaatctca cgagtgtcga tgatctgtgg ttcgtcttct acggccgtgt ggagcagatc
     3241 ctcaaggccc agggcctgct cccttcgggc tgggaggaga tcgcggtacg caagacccat
     3301 cgcgacgggc ggccgacgaa catccccaat cctgatttcg ccgcgcgcgg gtggcgcgcc
     3361 tacgtctggg acaacgtccc cggcgggggc gccgaggatc tcgcctaccg gctcgcgaat
     3421 ggcggctacg acgtcgtgct gagtcccgtg agca
//
LOCUS       DBNGZW010000691         3836 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_337727, whole genome shotgun sequence.
ACCESSION   DBNGZW010000691 DBNGZW010000000
VERSION     DBNGZW010000691.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3836)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3836)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3836
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_337727"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..1453)
                     /locus_tag="AC16JC_16325"
     CDS             complement(50..1453)
                     /locus_tag="AC16JC_16325"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020819696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HIR5166318.1"
                     /translation="MYSVAQFAAIAATALFIFALNWMNDPKTARRGVIAGVVGMSVAV
                     GATWVQPEILHHGWILLAIGAGFLVGVPLSLVPLTAVPQRTALSHAFGGLAAGLVGVA
                     EYNLWLMESPEQITTFRMAALVAEVILGFLTFTGSLMATGKLQEVKWIPQRPVTYPGQ
                     NIVNLGVLAGAIVLGVLVVMQPTAPWAHNAFLGVVGLALLFGVLLIVPIGGADMPTVI
                     SILNSYAGLSAVAMGFVLNNKLLVTAGALAGSSGLILSIIMCRAMNRSFTNVLFGAFG
                     TVQKSKAIGEQKVWKQESPEGAAQLLEQAAHVVVIPGYGMAVAQAQHKVRELYDQLTK
                     RGVTVKFAIHPVAGRMPGHMNVLLAEANIPYTALVEMDEINPEMPQCDVALVIGANDV
                     VNPAARYAKDSPIFGMPIIDADKAKTVLAIKRSKNPGFAGIDNELYVQDNTWMLFGDA
                     KGVIGDLVKQFAGGGIH"
     gene            complement(1453..1731)
                     /locus_tag="AC16JC_16330"
     CDS             complement(1453..1731)
                     /locus_tag="AC16JC_16330"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009059640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008750 - proton-translocating NAD(P)+
                     transhydrogenase activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P) transhydrogenase subunit alpha part 2"
                     /protein_id="HIR5166319.1"
                     /translation="MTDYWSMLFVFVLATFIGLGVIRRVSRLLYTPLMSLTNAISAIA
                     VVGSLVVTGADYPKVIRILGAVALFASMTNIVSGFLITDRMLKMFKKQ"
     gene            complement(1728..2819)
                     /locus_tag="AC16JC_16335"
     CDS             complement(1728..2819)
                     /locus_tag="AC16JC_16335"
                     /EC_number="7.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015151531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HIR5166320.1"
                     /translation="MKLGVPKETVAGERRVALVPDTVAKLVKDGHEVQVEPGAGSAAG
                     FPDDAYRAAGAKTGDPWAADIVCKVQKPTAAEVGKLRENGVLVGQFQAATSPDLLQQL
                     AQRRVTTFSLELLPRITRAQAMDVLSSQATAAGYKAVLLAAETTPKFFPMLVTAAGTL
                     SPARVLVLGAGVAGLQAIATARRLGAIVSAFDVRAAVKEQVESLGAKFLAMEEQVQAE
                     GAGGYAKELSADQHQRELEFLARSVKDADVVITTAAIPGKRAPVLVTRAAVEGMRSGS
                     VIVDLAAETGGNCELTQVGTTVVHQGVTILGPVNLPATLPLHASQMYSRNIATFLGHV
                     VKDGKLQPDMADEITKATCVTHEGKVLRS"
     gene            complement(2984..3427)
                     /locus_tag="AC16JC_16340"
     CDS             complement(2984..3427)
                     /locus_tag="AC16JC_16340"
                     /inference="COORDINATES: protein motif:HMM:NF017007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001540 - amyloid-beta binding [Evidence
                     IEA];
                     GO_function: GO:0004930 - G protein-coupled receptor
                     activity [Evidence IEA];
                     GO_process: GO:0097190 - apoptotic signaling pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HIR5166321.1"
                     /translation="MTGNLPTAYDSGEEPLGEAPQSSERSRSVALILGVLGGVFGFHR
                     FYVGRPQSAVFQALTLGGLGMWWLYDMVMILAGEFQDQDGLRLRNWNVAETAGIPGVN
                     ARTVSLLLQEMEQLQQQVGELAERMDFTERLLTQQRERDRLSKGS"
     gene            complement(3460..>3836)
                     /gene="trxA"
                     /locus_tag="AC16JC_16345"
     CDS             complement(3460..>3836)
                     /gene="trxA"
                     /locus_tag="AC16JC_16345"
                     /inference="COORDINATES: protein motif:HMM:NF012314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR5166322.1"
                     /translation="VGQGPKCGSCGRPILLDRPLHVTDETFQQVLQGTDIPVLVDFYA
                     DWCGPCRMMAPILDELAHDRLGEVLVLKLDTDRNPATPNGFGIRGIPTMILFQHGRET
                     ARQTGAAPRDQLDELLARAPSQ"
BASE COUNT          649 a         1319 c         1240 g          628 t
ORIGIN      
        1 gaacgaacgg ttcccgggac gtggcccacg gcgcagcgca ggggctgccc tagtggatgc
       61 cgccgcccgc gaactgcttc accaggtcgc cgatcacacc cttcgcatcg ccgaacagca
      121 tccaggtgtt gtcctgcacg tacagctcgt tgtcgatccc ggcgaatccc gggttcttgc
      181 tccgcttgat cgccagtacc gttttcgcct tgtcggcgtc gatgatcggc atgccgaaga
      241 tcggcgaatc cttggcgtaa cgcgccgccg gattcaccac gtcgttcgcc ccgatcacca
      301 gcgcgacgtc gcactgcggc atctccgggt tgatctcgtc catctcgacc aacgccgtgt
      361 aggggatatt ggcctcggcc agcagcacgt tcatgtggcc cggcatgcgc cccgccacgg
      421 ggtggatggc gaacttgacc gtcaccccgc gcttggtgag ctgatcgtac agctcgcgca
      481 ccttgtgctg ggcctgcgcg acggccatgc cgtagccggg gatcacgact acgtgcgccg
      541 cctgctcgag cagctgcgcc gcgccttcgg gcgactcctg cttccacacc ttctgttcgc
      601 cgatcgcctt gctcttctgt acggtgccga acgcgccgaa caggacattg gtgaacgacc
      661 ggttcatcgc ccggcacatg atgatcgaga ggatgagtcc actcgagccg gcgagcgccc
      721 cggcggtcac cagcagcttg ttgttgagga cgaaccccat cgctacggcc gagagcccgg
      781 cgtacgaatt caggatcgag atcaccgtcg gcatgtcggc gccgccgatc ggcacgatca
      841 gcagcacgcc gaacagcagg gccaatccca ccacgccgag aaacgcgttg tgcgcccagg
      901 gcgcggtcgg ctgcatcacc accagcacac ccagaacgat ggcgcccgcc agcaccccga
      961 ggttgacgat gttctggccc ggataggtga ccggacgctg cgggatccac ttcacctcct
     1021 gcagctttcc ggtcgccatg aggctgcccg tgaacgtcag gaaccccagg atcacctcgg
     1081 ccacgagggc ggccatgcgg aacgtggtga tctgctcggg agactccatg agccacaggt
     1141 tgtattccgc cacgcctacg agcccggcgg ccagcccgcc gaacgcgtgc gacagcgcgg
     1201 tccgctgcgg cacggcggtc agggggacga gcgagagcgg gacgcccacc aggaatcccg
     1261 cgccgatcgc gagcaggatc cacccgtggt gcaggatctc cggctggacc caggtcgccc
     1321 cgacggcgac gctcatgccc acgacgccgg cgatgacgcc gcggcgcgcg gtcttcgggt
     1381 cgttcatcca gttcagggcg aagatgaaca gggctgtcgc ggcgatggcc gcgaactgcg
     1441 cgacggagta catcattgct ttttgaacat cttgagcatg cggtcggtga tcaggaagcc
     1501 gctgacgata ttcgtcatcg aggcgaacag cgccacggcc cccaggatgc ggatcacctt
     1561 cggatagtcg gcgccggtca cgacgagcga gccgaccacc gcgatggccg aaatggcatt
     1621 cgtcagcgac atgagcggcg tgtagagcaa ccgcgagacc cgccggatca cccccagccc
     1681 gatgaacgtg gcgaggacga atacgaataa catcgaccag tagtcggtca tgaccggagc
     1741 acctttccct cgtgggtgac acacgtcgct ttggtgatct cgtccgccat gtccggctgc
     1801 agcttgccgt ccttcacgac atggcccagg aacgtggcga tgttgcggga gtacatctgg
     1861 ctcgcgtgca gcggcagcgt ggcggggaga ttcacgggcc cgaggatcgt gaccccctgg
     1921 tgcaccacgg tggtcccaac ctgcgtcagc tcgcagttgc cgccggtctc ggccgcgaga
     1981 tccacgatga ccgagccgga gcgcatcccc tccaccgccg cgcgggtcac caggaccggg
     2041 gcgcgcttcc cggggatcgc cgccgtcgtg atcaccacgt cggcgtcctt cacgctccgg
     2101 gccaggaact cgagctcgcg ctggtgctgg tccgccgaga gctccttggc gtagcccccc
     2161 gccccttccg cctgcacctg ctcctccatc gcgaggaact ttgcgccgag gctctccacc
     2221 tgctccttca ccgccgcccg tacgtcgaac gccgagacga tggcgccgag gcggcgggcg
     2281 gtggcgatcg cctgcagtcc cgcaacgccg gccccgagca cgagcacccg cgcgggcgac
     2341 aacgtcccgg cggcagtcac gagcatgggg aagaacttcg gggtcgtctc ggcggccagc
     2401 agcaccgcct tgtagcccgc ggcggtggcc tgtgaggaca gcacgtccat cgcctgggcg
     2461 cgggtgatcc ggggcagcaa ctcgaggctg aacgtcgtaa cccgccgctg ggccagctgc
     2521 tggagcaggt ccgggctggt cgccgcctgg aactggccga ccaggacgcc gttctcacgc
     2581 agtttgccga cttcggcggc cgtgggcttc tgcaccttgc agacgatatc ggcggcccac
     2641 gggtcgcccg ttttggcgcc ggccgcgcgg tatgcgtcgt cggggaagcc cgcggcgctt
     2701 cccgcgcccg gctccacctg cacctcgtga ccgtctttca ccagtttggc gaccgtgtcg
     2761 ggcacgagcg ccacgcggcg ctctccggcg acggtctctt tcgggacccc gagcttcatt
     2821 ctcgtcctcg atgggtggcg gtgtgaagaa gctataaggt atggataaag atgaaaggtt
     2881 gattgaatcg gtcgtaaagg catctttagg cgggaaacgg tacagagagg atgtcaaccg
     2941 gaacaagaag aaggacggac agatcggccc agcaggaatt acgctacgac cctttcgaca
     3001 accggtcccg ctcccgttgc tgcgtcagca gccgctcggt gaaatccatc cgctcggcca
     3061 gctctccgac ctgctgctgc aactgctcca tctcctgcag cagtagcgat acggttcgcg
     3121 cattgacgcc gggaattccg gccgtttccg ccacattcca gttgcggagg cggagcccgt
     3181 cctggtcctg gaactccccg gccaggatca tcaccatgtc gtagagccac cacatgccca
     3241 gcccgccgag ggtgagcgcc tggaacaccg cgctctgcgg gcggcccacg tagaagcgat
     3301 ggaagccgaa tacgcctccc aggacaccga gaatgagcgc gacactgcgg gagcgctcgg
     3361 acgactgcgg cgcctccccg aggggctctt cgcctgagtc gtacgccgtg ggcaggttcc
     3421 cggtcatgcg accaagatat cgcccacccg gccgggaggt tactgcgacg gcgcgcgcgc
     3481 gagcagttcg tcgagctggt cgcgcggggc ggcgcccgtc tggcgcgccg tttcccggcc
     3541 gtgctggaag agaatcatgg tcgggatgcc gcgaatgccg aatccattgg gcgtcgccgg
     3601 gttgcgatcg gtgtccagct tcaacaccag tacctcgccc aggcgatcgt gcgccagctc
     3661 gtcgagaatc ggcgccatca tccggcacgg tccgcaccag tcggcgtaaa agtccaccag
     3721 cacgggaatg tcggtgccct gcagcacctg ctggaacgtc tcatcggtga cgtgcagcgg
     3781 acggtcgagc aggatgggac gcccgcacga gccgcacttc ggtccctggc cgactc
//
LOCUS       DBNGZW010000692         2479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_542319, whole genome shotgun sequence.
ACCESSION   DBNGZW010000692 DBNGZW010000000
VERSION     DBNGZW010000692.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_542319"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..564)
                     /locus_tag="AC16JC_16350"
     CDS             complement(43..564)
                     /locus_tag="AC16JC_16350"
                     /inference="COORDINATES: protein motif:HMM:NF016089.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TQO small subunit DoxD"
                     /protein_id="HIR5166323.1"
                     /translation="MAYGSRSAGMDHPAEWLALLRVVVGLYFAKALITKVAFSPLPAT
                     SARWFATMPVLVSKQADGNPIVWYHDFLVNTVLPHARLFAHLTAWGEVAVGVFLTLGL
                     FTGLGSLIGLVLVVAYGLAVQWMSPTQQGFHIILFALMLAFFFARAGRRWGFDASLAR
                     SRPKSFLTRRPMA"
     gene            complement(564..>2479)
                     /locus_tag="AC16JC_16355"
     CDS             complement(564..>2479)
                     /locus_tag="AC16JC_16355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014896.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIR5166324.1"
                     /translation="QNERTAKYLDAIPERPFIRRRLEQLLTIGVVGVPTPARGRYVYL
                     RRDGAQNQPVLYVRDGVGGADRVALDPNALNAEGTTALDWYYPSEDGRLLAYGLSEDG
                     SERSVLHVLDLGTLEPRPDRIPDTRAADLAWLPDATGFYYTRYPAAGSVPEGEEQYHR
                     SVYLHRLGTDPETDPLIFAPAEPVHWPGVSLSRDGRWLLISVSRTFDETDLYLRDLVA
                     DGPLVPVARDLPALFEGQVVGDRLYIRTNVEGPTYGLYAADPESPERADWRTLVAPRP
                     DAVLEGVSITRAHLALNYLERATSRLVLTDLDGAGPRDIPLPAIGSVFGTGSEPDGDE
                     LFYGFSSYGVPPSVYRLDLPAGTQTLWQRVEADVDPARFEVRQVTFSSRDGTPVTMFV
                     VAPRGLALDGNNPTYLTGYGGFNISMTPAFSRSLLLWLEHGGVLAVPNLRGGGEYGER
                     WHQGGMLGTKQNTFDDCIAAAEWLIRERYTSPERLALAGGSNGGLLAGALLTQRPDLF
                     RAVIIQVPLLDMLRYHHFLIAKLWIPEYGSPEDPEQYLWLRGYSPYHHVRDDTPYPAV
                     LLATAESDTRVDPMHARKMTARLQAATSSGRPILLRLESKAGHGAGKPLAKLVDELTD
                     SWSFVFHELGVKL"
BASE COUNT          410 a          822 c          908 g          339 t
ORIGIN      
        1 agatgccctg gggtgtggcg gtcggcagcc gctgattcgc cgctacgcca tcggccgccg
       61 cgtcaggaac gacttcggcc gcgagcgcgc gagcgaggcg tcgaatcccc accgccggcc
      121 ggcgcgcgcg aagaagaacg cgagcatcag cgcgaacagg atgatgtgga atccctgctg
      181 ggtcggggac atccactgca ccgccagtcc gtacgccacg acgagcacga gaccgatcag
      241 cgagccgagg ccggtaaaga gaccgagggt gaggaagacg ccgaccgcca cctcgcccca
      301 cgcggtgagg tgcgcgaaga gccgggcatg cggcaggacg gtgttgacca ggaaatcgtg
      361 gtaccacacg atcggattgc cgtccgcctg cttcgacacg agcaccggca tcgtggcgaa
      421 ccaacgggcc gacgtcgccg gcagcggcga gaacgccacc ttggtgatca gcgccttggc
      481 gaagtagaga cccaccacga ccctgaggag cgccagccac tccgccgggt ggtccattcc
      541 cgccgaccgc gatccgtagg ccatcagagt ttcactccga gctcgtgaaa gacgaagctc
      601 cagctgtcgg tcagctcgtc caccagcttc gccagcggct tgcccgcgcc gtgccccgcc
      661 ttcgactcga gcctgaggag aatcgggcgc cccgaggaag tcgccgcctg gagccgcgcc
      721 gtcatcttcc gcgcgtgcat cgggtccacg cgggtatcgc tctccgcggt ggcgagcagc
      781 acggcgggat acggcgtgtc gtcccgcacg tgatggtacg gggaatagcc ccgcagccag
      841 aggtactgct cgggatcctc cggggagccg tactccggaa tccacagctt cgcgatcagg
      901 aagtgatgat agcgcagcat gtccaggagc gggacctgga tgatcaccgc gcggaagaga
      961 tcgggacgct gggtgaggag ggcgccggca aggagaccgc cgttggaccc gccggccagg
     1021 gcgagccgct ccggcgaggt gtagcgctcc cggatgagcc attcggcggc tgcgatgcag
     1081 tcgtcgaagg tgttctgctt ggtgccgagc atcccgccct ggtgccagcg ctccccgtac
     1141 tcgccgccac cgcgcaggtt cggcaccgcc agcaccccgc cgtgctccag ccagagcagg
     1201 agcgaacggg agaacgccgg cgtcatgctg atgttgaagc cgccgtagcc ggtcaggtac
     1261 gtcgggttgt tgccgtcgag tgcgaggccg cgcggcgcga ccacgaacat cgtcaccggc
     1321 gtcccgtcgc gcgaggagaa cgtcacctgc cgcacctcga atcgtgccgg atcgacatca
     1381 gcctcgaccc gctgccagag cgtctgcgtg cccgccggca ggtcgagccg gtacacgctg
     1441 ggcggcacgc cgtaggagga aaatccgtag aagagctcgt cgccgtcggg ctcggagccg
     1501 gtgccgaaga cgctgccgat cgcggggagc gggatgtcgc gcgggccggc accgtcgagg
     1561 tcggtgagca ccagccggga ggtggcgcgc tcgagatagt tgagcgcgag gtgggcgcgg
     1621 gtgatcgata ccccttcgag caccgcatcg ggccggggtg cgaccagcgt gcgccagtca
     1681 gcgcgctccg gtgattccgg gtcggcggcg tagaggccgt acgtcggccc ttcgacattg
     1741 gtgcggatgt acagccggtc gcccacgacc tgcccctcga agagagccgg aaggtcgcgc
     1801 gccaccggca cgaggggacc gtcggccacg aggtcccgga gatacagatc ggtctcgtcg
     1861 aaggtgcggg agaccgagat cagcagccag cggccgtcgc gcgacagact gacgcccggc
     1921 cagtgcaccg gctccgccgg cgcgaagatc agcggatcgg tctcgggatc ggtcccgagc
     1981 cggtggagat agaccgagcg atgatactgc tcctcgccct cgggcacgga gccggcggcg
     2041 gggtagcgcg tgtagtagaa gccggtcgcg tcgggcagcc aggcgaggtc ggcggcgcgg
     2101 gtgtcgggaa tgcggtcggg tcgcggctcg agcgtgccga gatcgagcac gtgcaggacg
     2161 ctccgctcgc tgccgtcctc ggagagtccg taggcgagca ggcggccgtc ctcgctcggg
     2221 tagtaccagt cgagggcggt ggtgccctcc gcattcagcg cgttgggatc gagcgccacc
     2281 cggtcggccc cgccgacgcc gtcgcggacg tagagcaccg gctgattctg cgctccgtcg
     2341 cgtcggagat agacgtagcg cccgcgggcc ggcgtcggca cgccgacgac gccgatcgtg
     2401 agcagctgct ccagccggcg gcggatgaac ggccgctcgg gaatggcgtc gagatacttc
     2461 gcggtgcgct cgttctggc
//
LOCUS       DBNGZW010000693         3288 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1495928, whole genome shotgun sequence.
ACCESSION   DBNGZW010000693 DBNGZW010000000
VERSION     DBNGZW010000693.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3288)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3288)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3288
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1495928"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..521)
                     /locus_tag="AC16JC_16360"
                     /pseudo
     CDS             complement(1..521)
                     /locus_tag="AC16JC_16360"
                     /inference="COORDINATES: protein motif:HMM:NF039641.6"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6064 family protein"
     gene            complement(527..1738)
                     /locus_tag="AC16JC_16365"
     CDS             complement(527..1738)
                     /locus_tag="AC16JC_16365"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR5166325.1"
                     /translation="MGALPVAPGRGPGVSPPELGPSGRDPFDPEFCRALERKVLGPII
                     RDYFRARVVGAERLPKRGPVILAANHSGNAFPHDGIVLDAALWHRDGLRSSLKFRTVY
                     EPELTLAWWMRPFGIDDFWRRGGGVDMTFDNFERLILRGERVLYFPEGVPGIGKGFNR
                     RYQLQPFKTSFVLLAGRYNVPVYPVYAINCEWVHPFGYCFPPLDRLMQRVFHVPFLPL
                     PIGLLACVFPWMWYLAFPCRMVFIVGKPIDVPGLLWLEGVTDFEHPDRGALRRVAEHI
                     RRLMQGEMDGLVAEHGRQPYHARSLGRSLRRAWGRWRMVLPWGWPVAYLRLERDRQRP
                     PARNRLHAVLRDWDLIGFYLPFGWPFLSLFRALRRPPYGYRGLSRKEKRERMGNYLWR
                     LSERPLPPVPV"
     gene            complement(1773..>3288)
                     /locus_tag="AC16JC_16370"
     CDS             complement(1773..>3288)
                     /locus_tag="AC16JC_16370"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC1 kinase family protein"
                     /protein_id="HIR5166326.1"
                     /translation="SDPPAHGFVFGLQRAFAVLARRLVDRSLVNEPLSVQFRRRLELL
                     GPTYIKLGQILSLREDLLPRPVTDELKYLLNALPAVPFDTFRRLVERDLHRPLEEAFL
                     AVDPVPIGSASIAQIHRATTLDGDPVVIKAVKPGIPDILRRDARLLRMLGRVLQWLAP
                     RYQPRRIIDEFVEYTQREVDLRQEAENIETLAGNFHDLPDVIFPAVYPELSGPQVLCM
                     EYVDGLRPDTPQAQALPRKARERLVDLGAASIIRMIYRDGFFHADLHPANLLILPGTK
                     VGFIDLGMVGRLDGELRRTLLYYYYSLVTGDAENAARYLTAVAEAGRGSDPLGFRREV
                     ADISRRWRRHATFQGFSLGRLVLESLSRGARYRMYFPVELVLMVKALITFEGVGNVLL
                     PGFDVAEVSRRHIREIFLLQFSPWRVLQEELRGAPDLMDALVKVPLLVTEGVRVLEKS
                     TKRAPENPFAGVRGTLIAGFCLVAGAILMAFGGPWPLWVGLFALAPLLALRRGT"
BASE COUNT          608 a         1176 c         1106 g          398 t
ORIGIN      
        1 ccaggcccac ggaatcagca tgatcgcgac gggcggccgg ttcacggtcc aaagcaggag
       61 ggcgagcgtc gcgatcgtcg tcgggcacgg caccccgaac gtcggcgccg ccgggtagcg
      121 atgccccagc gcccacccga tggccggtag accgcgaacg cgtacaccag cagcgcgagc
      181 gccagtccgc cgcgcagcga ccgtggcgcc cgaaacgcga gacgcggcga ccgaaccgca
      241 gcccaggcga gcaatacggc ctcgaccacg aacgccgcgc cgaacagcgc cgccgccgga
      301 ttgatgggac ggaagaagcg ccagtggtac tccaccccca tccacagcca cagccccgcg
      361 agcaccagcc cgatcgcccg atccgaccac cgcatcggcc acaacgccag ggccgccgtc
      421 ccgatcccca gtgcgatcaa caggatctgc accggcgcca cggcctcgtt gtacgcggcg
      481 aacaccgcga ggaattcgtc ccgggaaaac ggcagcggca tcccgctcag accggcaccg
      541 gcggcagcgg acgctcggac agccgccaca gatagttgcc catccgctcg cgtttctcct
      601 tccgcgagag gccgcgatac ccgtacggcg ggcggcgcaa cgcgcggaag agagagagga
      661 agggccagcc gaacggcagg tagaacccga tcagatccca atcccgcaga accgcgtgca
      721 gccggttgcg ggccggcggc cgctggcggt cacgctcgag gcgcaggtag gccaccggcc
      781 atccccaggg cagcaccatg cgccaccgtc cccaggcgcg gcgcagcgac cgccccagcg
      841 agcgcgcgtg gtagggctgc cgcccgtgct ccgccaccag accatccatc tcgccctgca
      901 tcaggcgccg gatgtgctcc gccacccgac gcagcgcgcc gcgatcgggg tgctcgaaat
      961 cggtgacgcc ctcgagccag aggagcccgg ggacatcgat cggcttcccc acgatgaaca
     1021 ccatccggca cgggaaggcg agataccaca tccaggggaa gacacaggcc aacagaccga
     1081 tcggcaacgg caggaagggc acgtggaaca cacgctgcat cagccggtcg agcggcggga
     1141 agcagtaccc gaaggggtgc acccactcgc agttgatcgc gtacaccgga tacacgggga
     1201 cgttgtagcg gccggccagg agtacgaagc tcgtcttgaa cggctgcagc tggtaacggc
     1261 ggttgaatcc cttcccgatc cccggcacac cctcggggaa atacagcacg cgctcaccgc
     1321 gcaggatcag ccgctcgaag ttgtcgaacg tcatgtccac gccgccgccg cgccgccaga
     1381 agtcgtcgat gccgaacggg cgcatccacc acgccagcgt caactcgggc tcgtagacgg
     1441 tgcggaactt gagcgaggac cgtagcccgt cccggtgcca cagcgccgcg tccagcacga
     1501 tgccgtcgtg cgggaacgcg ttcccggagt gattcgcggc gaggatcacc ggcccgcgtt
     1561 tcggcaggcg ctcggcgccg accacccggg cgcgaaagta gtcccggatg atcggcccca
     1621 ggaccttgcg ctccagcgcg cgacagaact ccgggtcgaa cggatcccga ccggatggtc
     1681 ccagttcggg tgggcttacg ccggggccac gccccggcgc taccgggagc gcgcccaccg
     1741 ggggctcctt cacctcggcc cgcgcaccca cgtcacgtcc cccgccgcaa tgccagcagc
     1801 ggcgccaggg cgaacagccc gacccacagc ggccagggac cgccgaacgc catcaggatc
     1861 gcgccggcca ccagacagaa ccccgcgatc aacgttcccc gcactccggc gaacggattc
     1921 tcgggagcgc gtttggtgga tttctcgagc acgcgcacgc cctcggtcac cagcagcggc
     1981 actttcacca gcgcatccat caggtcgggg gcgccgcgca gctcttcctg cagcacacgc
     2041 cacggactga attgcagcag gaagatctcc cggatgtggc ggcgcgagac ctcggccacg
     2101 tcgaaccccg ggagcaatac gttccccacg ccttcgaagg tgatgagcgc cttgaccatg
     2161 aggacgagct cgaccgggaa gtacatccgg tagcgcgccc cgcgcgacag cgactcgagc
     2221 accaggcggc cgagagagaa tccctggaac gtggcgtggc ggcgccagcg ccgcgagatg
     2281 tccgcgacct cgcgccggaa tcccagtgga tcgctccccc ggccggcctc ggcgaccgcg
     2341 gtgaggtagc gggccgcgtt ctccgcgtcg cccgtcacga ggctgtagta gtaatacagc
     2401 agcgtgcggc gcagctcgcc gtccagccgg ccgaccatcc cgagatcgat gaagccgacc
     2461 ttcgtgcccg gcaggatcag caggttcgcc ggatgcagat cggcgtgaaa gaagccgtcc
     2521 cggtagatca tccggatgat cgacgcggcg ccgaggtcta ccagccgctc gcgcgctttg
     2581 cgcggcaggg cctgcgcctg aggcgtgtcg ggccgcaggc cgtcgacgta ctccatgcac
     2641 aacacctgcg ggcccgaaag ctcgggatag accgccggga agatcacgtc gggaagatcg
     2701 tggaagttgc cggcgagcgt ctcgatgttt tcggcttcct gccgcagatc gacctcgcgc
     2761 tgggtgtatt ccacgaactc gtcgatgatg cggcgcggct gatagcgcgg agccagccac
     2821 tgcagcaccc ggccgagcat gcgcagcagg cgcgcgtcac gccgcaggat gtcgggaatg
     2881 cccggcttga ccgccttgat gacgacggga tcgccgtcga gcgtggtggc gcggtggatc
     2941 tgcgcgatcg aggccgagcc gatcgggacg gggtcgacgg cgaggaatgc ctcctcgagc
     3001 ggccggtgca gatcgcgctc cacgagccgg cggaacgtgt cgaacggcac cgccggcagc
     3061 gcgttgagca ggtacttcag ctcgtcggtc accggccgcg gcaacaggtc ctcgcgcagg
     3121 ctcaggatct gcccgagctt gatgtaggtg ggcccgagca gctcgagacg acggcggaac
     3181 tggaccgaga gcggttcatt gacgagcgat cgatccacca gccgccgcgc gaggacggcg
     3241 aacgcgcgct gcaggccgaa gacgaagccg tgcgccggcg gatcgctt
//
LOCUS       DBNGZW010000694         2397 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1009841, whole genome shotgun sequence.
ACCESSION   DBNGZW010000694 DBNGZW010000000
VERSION     DBNGZW010000694.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2397)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2397)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2397
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1009841"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1476)
                     /locus_tag="AC16JC_16375"
     CDS             complement(34..1476)
                     /locus_tag="AC16JC_16375"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HIR5166327.1"
                     /translation="MKAQFKFLSGARAGQVETFQKAYLGLGRHPLSDVRFDAERDLDV
                     SSRHAAIITRSDGFVLQDLGSKNGTYVNGQRIEGDRVLRDGDVIGFGAKGPALEFHIV
                     EGDVTAEPTATAAAAAGRLSQPKQVVPAVGPLTREPPGRRPRSRSTTAIIEMATRPMR
                     RTVAASVALVVVVIGLAGFVLWRGARERQADIARIKAWADSVTEQAQRTAQQFQTEVQ
                     GLREANQQAIAEIERLHRELTQAGSDAGAVSRLRGQIEQAQARQQLIAGAGAVDYRTI
                     SARNQDAVALLIVEFSDTERFSGTAFAVDSQGTLVTNKHVLVGVNGDRMPRRIAVKFS
                     GSRQWFQGRFIGTAEGADIGVLRVIIAGGTPRVLGLQRDPRSLQRGDPVAIIGYPLGL
                     DLPMEGQGTDVVASPTLTVGTTSKVLPTLVQVDGYGAPGSSGSPIFDHDGRVIAVLYG
                     GVQESNGKIILAVPVSAVADYLSKLGIRTR"
     gene            complement(1473..>2397)
                     /locus_tag="AC16JC_16380"
     CDS             complement(1473..>2397)
                     /locus_tag="AC16JC_16380"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HIR5166328.1"
                     /translation="APLSEREYELGDRGLLLVVADGMGGAAAGELASEMATDTIYKQL
                     LGSWLREEDATGQRFALRLKEAVETANQQIHEYAKAHPENRGMGTTATTVGILGDHLY
                     LTQVGDSRAYLVRDGAAHQLTKDQSLMQRLVEAGELTEEEAAQSERRNIILQALGPDV
                     RVKVDLTHQEIRRGDTVVLCSDGLSGQVKKEEIARMVSETRDLTAVCDRLVALANERG
                     GPDNITVVVARCDGDGLRAAASAEEVGHQVFPLIDTEASTDPVPVYRGSKPPVPAAKQ
                     GQDIRWKLLLLLIGVALLVAAMVWARSAATP"
BASE COUNT          304 a          843 c          881 g          369 t
ORIGIN      
        1 cccccccccc cccccccccc cccactcccc gctctatcgc gtccggatgc ccagtttcga
       61 gaggtaatcc gccaccgcgc tcaccggcac ggcgaggatg atcttcccgt tggattcctg
      121 caccccaccg tacagcacgg cgatgacgcg gccgtcgtgg tcgaagatcg ggctgcccga
      181 ggagccgggg gcgccgtagc cgtccacctg gaccagggtg ggcaggacct tgctggtggt
      241 gccgacggtg agcgtgggcg aggcgacgac gtccgtgccc tggccctcca tcggcaggtc
      301 gaggccgagc gggtagccga tgatggcgac ggggtcaccg cgctgcaggc tacgcgggtc
      361 gcgctgcagg ccgagcaccc gcggcgtgcc gccggcgatg atcacgcgca gcacgccgat
      421 gtcggcgccc tcggcggtgc cgatgaaacg cccctggaac cactgacggc tgccggagaa
      481 cttgaccgcg atgcggcgcg gcatcctgtc gccgttcacc cccaccagga cgtgcttgtt
      541 ggtgacgagc gtgccctgcg aatcgaccgc gaacgccgtg cccgagaacc gctcggtatc
      601 cgagaactcg acgatcagca gcgccacggc gtcctggttg cgggcggaga tggtgcggta
      661 gtccaccgcg ccggcgccgg cgatgagctg ctggcgcgcc tgcgcctgct cgatctgccc
      721 gcgcagccgg gagacggcgc cggcgtcgct gccggcctgg gtgagctcgc ggtgcagccg
      781 ctcgatctcg gcgatcgcct gctgattggc ctcgcgcagc ccctggacct ccgtctggaa
      841 ctgctgcgcc gtgcgctgcg cctgttccgt cacgctgtcg gcccaggcct tgatgcgcgc
      901 gatgtcggcc tgccgctcgc gcgcgccgcg ccagaggacg aacccggcga gcccgatgac
      961 gaccaccacc agggcgaccg acgccgccac ggtgcggcgc atggggcgcg tcgccatctc
     1021 gatgatggcg gtggtggaac gcgagcgcgg gcgccgcccg gggggctcgc gcgtgagcgg
     1081 ccccacggcg ggaacgacct gcttcggctg cgacaggcgg ccagcggccg cggcggcggt
     1141 ggcggtgggc tccgccgtga cgtcgccctc cacgatgtgg aattcgaggg ccggcccctt
     1201 ggcgccgaag ccgatcacgt cgccgtcccg caacacgcgg tcgccttcga tccgctgccc
     1261 gttgacgtag gtgccgttct tgctgcccag gtcctggagc acgaagccgt cgctccgggt
     1321 gatgatggcc gcgtggcgcg acgacacgtc gaggtcgcgc tcggcgtcga agcgcacgtc
     1381 ggagaggggg tggcggccga ggccgaggta ggccttctgg aacgtctcga cctgcccggc
     1441 gcgcgcgccg gacaggaact tgaactgggc tttcacggcg tggcggccga cctcgcccag
     1501 accatggcgg cgacgagcag cgccacgccg atgagcagga gcaggagctt ccagcggata
     1561 tcctgaccct gcttggccgc gggcaccggc ggcttgctgc cgcgatacac gggcacgggg
     1621 tccgtgctgg cctcggtgtc gatcagcggg aagacctggt ggccgacttc ctccgcgctg
     1681 gcggcggcgc gcagtccgtc gccgtcgcac cgggcgacga cgacggtgat gttgtccggc
     1741 ccgccgcgct cgttcgcgag cgctaccagg cggtcgcaga ccgccgtgag gtcgcgcgtc
     1801 tcggaaacca tgcgcgcgat ctcctccttc ttgacttgtc ccgacaggcc gtcggagcag
     1861 agcaccacgg tgtcgccgcg gcggatctcc tgatgcgtga gatcgacctt gacccggacg
     1921 tcggggccga gcgcctggag gatgatgttg cggcgctcgc tctgcgcggc ttcctcctcg
     1981 gtgagctcgc ccgcttcgac gaggcgctgc atcagcgact ggtccttggt gagctgatgg
     2041 gcggcgccgt cgcgcaccag gtaggcgcgg ctgtcgccga cctgggtgag gtagaggtga
     2101 tcgcccagga tgccgacggt ggtggcggtg gtgcccatgc cgcggttctc ggggtgggcc
     2161 ttggcgtatt cgtggatttg ctggttcgcg gtttcgacgg cttccttgag gcgcagcgcg
     2221 aagcgctggc ctgtcgcgtc ctcttcgcgc agccacgagc cgagcagctg cttgtagatg
     2281 gtgtcggtgg ccatctcgct cgcgagctcg ccggcggcgg cgccgcccat gccgtcggcg
     2341 acgacgagca gcagcccgcg gtcgcccagc tcgtactcgc gctcggacag gggcgcg
//
LOCUS       DBNGZW010000695        10337 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_65409, whole genome shotgun sequence.
ACCESSION   DBNGZW010000695 DBNGZW010000000
VERSION     DBNGZW010000695.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 10337)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10337)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10337
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_65409"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /gene="xylA"
                     /locus_tag="AC16JC_16385"
     CDS             <1..1180
                     /gene="xylA"
                     /locus_tag="AC16JC_16385"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007907138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HIR5166329.1"
                     /translation="PRVTQDAYTPRPEHHFTFGLWTVGNPGRDPFGEPVRQPLKPTDS
                     VRKLAELGAYGVNLHDEDLVPRDATSAERDRIVREFKQVLAETGMKVPMATTNLFSDP
                     AFKDGAFTSNDARVRAYALQKTMAAIDLGVELGATIYVCWGGREGVETNAAKDPRTAL
                     AWYRDAVDYLCEYVRSQGYDLRFALEAKPNEPRGDIFLPTTGHMLAFIGTLDHPEMVG
                     VNPEVAHERMAGLDFCHAVAQALAAGKLIHIDLNGQQVGRFDQDLRFGSDDPKGAFFL
                     VKLLEDARWPGMRHFDSHAYRTEDEAGVWDFAAGSMRTYLILKEKVAQFNADAEIQQL
                     LKELGRRGGGGDGEGLVFSKEGARALRGRQFDLAALRGQGYAYERLDQLTLELLLGVR
                     "
     gene            1181..2665
                     /gene="xylB"
                     /locus_tag="AC16JC_16390"
     CDS             1181..2665
                     /gene="xylB"
                     /locus_tag="AC16JC_16390"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013915336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - D-xylulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HIR5166330.1"
                     /translation="MFLGLDVGTSGVKAILVNAAGSVESASTIPLTLDTPQPGWAEQH
                     PDQWWSASCDAIRGVLAQRPGERVAAVGISGQMHSSVFLDRAGAVIRPALLWCDGRTT
                     PECREIVERAGGEDRLRAWVCNAALEGFTLPKVLWLRNHEPAAFARLATVLLAKDFVR
                     LRLTDSLATEPSDASGTLMFDLAAMRWSGDIMGAVGLPVSLLPQVGGSAEVLGRVTAQ
                     ASTLTGLPTGTPVVGGGADNACGAAGVGVVTPGEAVASWGTSGTVLAPTREPRVDPAM
                     RAHTFCHVAPGLWYVIGVVLSAGGAFAWYRDQLARDLAGTGQRDDRLVEEAAAVPAGA
                     EGVTFLPYLQGERTPHRDASLRGAFLGLSLAHSRAHLTRAVLEGVCFALRDSLVILQE
                     LGMAPRYLLLTGGGAKSATIRRLLADIFGLPVTTVNREEGPAYGAALLAAVGAGAFPD
                     LAAATQRTLERSPLMEADPEAHRQYAKVYERFRNWYGAATFSAR"
     gene            complement(2806..3720)
                     /locus_tag="AC16JC_16395"
     CDS             complement(2806..3720)
                     /locus_tag="AC16JC_16395"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR5166331.1"
                     /translation="MYKSLLLPLNALCASILWSQTPSSAPAQSPRSTQATLSVHWEEL
                     TAAEFREAITRAQGTCLLPFGIMEKHGPHLPLGNDLLNVRYVALNAAQQEYTVVFPEY
                     YFGQIFEAKHQPGTVSYSRRLQLDLLQETTDEMARNGCKKIVIVNGHGGNNSLLPYFA
                     QTQLDTPHDYVVYVQGLPEGGPDEPKHKTNPATDMHAGESETSLSMIARPDLVHLDRA
                     AQESGADQARQHLPEGLYTGIWWYARFPNHYAGEGAAASRELGQYEARTWISGLVRAI
                     RAVKADQESQRLQNEFYERSRHPLDTPQ"
     gene            complement(4147..6483)
                     /locus_tag="AC16JC_16400"
     CDS             complement(4147..6483)
                     /locus_tag="AC16JC_16400"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HIR5166332.1"
                     /translation="MSPVLLGVAVAAVAPAPQAPVAPPRAADSAVVVDGRLDEPVWAR
                     AARLRGFSQYLPNDDRPADDSTTVLVWYAPQALYFGIRAYQDSALVRATLADRDKISS
                     DDYVEILLDTYNDQRQAYVFGVNPLGVQADGTLRDAARQSIDITQSAVAGAYTVDLGP
                     DYVYQSKGRQTAWGYEVELRIPFKTLRYQTRDPQDWGLNAIRVVQATGHQFTWTRVLQ
                     TQASFLAQSGRLTGLTALRRGLVLDATPEVTSTVTGAPRPRGEGWHYSGGAPRIGATV
                     RWGVTANLTLDATAHPDFSQVEADVPQIQFDPRVALNFPEKRPFFLDGVELFQTPVQL
                     IYTRRLVDPLAALKLTGKVGATTVALLSGVDATDASATGTDHPVMNALRLRRDLGGHS
                     NLGMVYTDRVDGGGVNRVAALDGTLTLGDAWALRFQGAGSATRDSASAPVRWAPLWRA
                     DLVRSGRRFGLDLRSAAVGDDFRAGVGFVSRTGFVSTSVTPSYTIVGVPGSLIESFTA
                     NAYLALTWNQFRQFASRHVPTTRQDDMSAGFTLRGGWQVGMGVSVESFGYPSPLYANY
                     WVERVNAATVDTVPFVGQPQIANLDVGVNLQTPRVQSLSLSGFLLVGNDEDFYEWASG
                     RVVLGTVDLTWRPTDRLRAELIYNHQQVNRRDDGSQVARIRVPQLRLEYQLTRPIFFR
                     LIGQYTASEVAALRDDARTNGAILLRDPVTGTFTRSAPSETNALRIDWLFSYRPTPGT
                     VVFVGYGSRLDDGAPFRFRPLSRVTDGFFLKLSYLLRK"
     gene            complement(6551..7498)
                     /locus_tag="AC16JC_16405"
     CDS             complement(6551..7498)
                     /locus_tag="AC16JC_16405"
                     /inference="COORDINATES: protein motif:HMM:NF017390.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M56 family metallopeptidase"
                     /protein_id="HIR5166333.1"
                     /translation="MLGWMLYCVLLAACAAAAALPLERIVRHAARPTRWVWALALAAS
                     VLVSEIARVREVGILPVILPRAAAAPWLRAPAVTFAQTLHAFDRWDAHLTIAAWGATA
                     LFAAWFILSFARLLQRRRTWRAAIVDGHAVLVSDADGPAVFGIGRGTIVLPRWALDAG
                     DRARALMLAHELEHQQSGDTRVLLLPLVAVLLQPWNPAVWWLAGRLRLAIEVDCDARV
                     LDRGADLHSYGLVLLEAGSRGGGAPLPAPALSTPPSWLERRIRAMCERGGERVRVGWR
                     MLVVVLALSAAAFLPEPAGLHCALQALGFPVGHPAGRTN"
     gene            complement(7498..7872)
                     /locus_tag="AC16JC_16410"
     CDS             complement(7498..7872)
                     /locus_tag="AC16JC_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017464087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HIR5166334.1"
                     /translation="MDLTFTNRELDVMAILWDLGSATVTEVRDRLDDDLAYTTVLTVL
                     RTLESKGYVDHRAAGKAHRYAPTVARNAAGKSALRRLTDKVFSGSPELVLTQLVSGRR
                     LSADELKRMRQLLDQRLKESRS"
     gene            complement(7981..8637)
                     /locus_tag="AC16JC_16415"
     CDS             complement(7981..8637)
                     /locus_tag="AC16JC_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011685595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation efflux transmembrane domain-containing
                     protein"
                     /protein_id="HIR5166335.1"
                     /translation="MTILEPDQPLRRVSLLRRGVLLEVATVTYNALEGLVAIAAGLVA
                     GSVALTGFGVDSVIEVASGVVLWWRLRAELGSAPVGPRAEAAAARGAGALLLALAAYL
                     VVESGRRLIVRDRPEPSAVGVALTALSLIVMPLLARAKLRTARALESAALRADAHETI
                     VCAWLSLTTLVGLGLNALLGWWWADPVAALVMLPLIVREGVEAWRAKSCADDETEAAA
                     "
     gene            complement(8762..9319)
                     /locus_tag="AC16JC_16420"
     CDS             complement(8762..9319)
                     /locus_tag="AC16JC_16420"
                     /inference="COORDINATES: protein motif:HMM:NF024769.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HIR5166336.1"
                     /translation="MAELALLDIRTRAQWRAWLGKHHTSSRGMWVVFHKAHTGVQSMS
                     YEDFVREALRFGWIDSLVKRLDDDRFAIEATPRKATSRWSDINRRRWMELKRAGLLTA
                     AGLAAAPTENRYAPKQRIPELPRYIGKAMKSNPEAWSFFQKLPRRERHNFVVWIHIAK
                     RPETRERRIRESIALLAAGRKLGLK"
     gene            complement(9332..9982)
                     /locus_tag="AC16JC_16425"
     CDS             complement(9332..9982)
                     /locus_tag="AC16JC_16425"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014784200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR5166337.1"
                     /translation="MSPNWKTTFSIAAAAVARRDRVMAGLMKRTGPFRLPPTGRTHFA
                     ALAESIHYQQLAGAGAAAIHGRFIALFDGKPVAEAVLAMHPRRLRAAGLSQSKVASLR
                     DLAAKVMDGTVPLRGIARRSDEDIITRLSVVRGIGRWTAEMFLIFQLRRLDVWPVGDY
                     GVRLGYALAYGLRKLPTPARLQAEGERFRPYRTVAAWYCWRAVHEHRGQGGDADSL"
     gene            10158..>10337
                     /locus_tag="AC16JC_16430"
     CDS             10158..>10337
                     /locus_tag="AC16JC_16430"
                     /inference="COORDINATES: protein motif:HMM:NF016144.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HIR5166338.1"
                     /translation="MQSTPERYFFRRGEPRQYHLHLTPLGSQVWRDQLTFRDLLRLDP
                     VVASAYAALKRELAAR"
BASE COUNT         1667 a         3480 c         3584 g         1606 t
ORIGIN      
        1 gccacgagtc acgcaggacg cgtatacgcc caggccggag caccacttca ccttcgggct
       61 ctggacggtg gggaatcccg gccgcgatcc cttcggcgag ccggtgcggc agccgctgaa
      121 gcccacagac agcgtgcgga agctggcgga actcggcgcc tacggcgtca acctccacga
      181 tgaggacctc gtgccgcgag acgctacctc ggccgagcgg gatcggatcg tgcgggagtt
      241 caagcaggtg ctcgccgaaa cggggatgaa ggtgccgatg gcgaccacca atctcttctc
      301 cgaccccgcg ttcaaggacg gtgcgttcac cagcaacgac gcgagggtcc gggcgtacgc
      361 gctccagaag accatggccg cgatcgactt gggggtggag ctcggcgcca ccatctacgt
      421 ctgctggggt gggcgggaag gggtcgagac caacgcggcc aaggacccgc gcaccgcgct
      481 cgcgtggtac cgcgacgccg tcgactacct gtgcgagtat gtccggtcgc agggctacga
      541 cctccggttc gcgctcgaag ccaagcccaa cgagccgcgc ggcgacatct tcctgccaac
      601 cacgggccac atgctcgcgt tcatcggcac cctcgaccac cccgaaatgg tcggcgtcaa
      661 ccccgaggtc gcgcacgagc ggatggccgg gctcgatttc tgtcacgccg tagcgcaagc
      721 gctcgccgcc ggcaagttga ttcacatcga cctgaatggg cagcaggtgg gacggttcga
      781 ccaggatctg cgcttcggca gcgacgaccc gaagggcgcg ttcttcctcg tcaagctgct
      841 cgaggacgcg cgctggcccg ggatgcgaca cttcgacagc cacgcctacc ggaccgagga
      901 cgaggcaggc gtctgggact tcgccgccgg cagcatgcgc acctatctga tcttgaagga
      961 gaaagtcgcg cagttcaacg ccgatgccga gatccagcag ctgctgaaag agctcggtcg
     1021 tcgtgggggg ggaggcgatg gcgagggtct cgtcttcagc aaggaaggag cacgggcgtt
     1081 gcgcgggcgg cagttcgatc tcgcggcgct gcgcggccag ggatacgctt acgagcggct
     1141 cgatcagctg accctggagt tgctgctggg agttcgctag atgttcctcg gcctcgacgt
     1201 cggcacgagc ggcgtgaagg ccatactcgt gaatgcggcg ggatcggtcg aatccgcgtc
     1261 gaccattccc ctgaccctcg acacgccgca accgggctgg gccgagcagc acccggatca
     1321 gtggtggagc gccagctgcg acgccatccg gggcgtgttg gcccaacggc ccggcgagcg
     1381 cgttgccgcc gtgggcatct cgggccagat gcattcgtcc gtcttcctcg accgtgccgg
     1441 agccgtgatt cgtcccgcgc tcctgtggtg tgacgggagg acgaccccgg agtgcaggga
     1501 gatcgtcgag cgcgcgggag gtgaagaccg gctgcgcgcc tgggtgtgca acgccgcgct
     1561 cgaaggcttc acgctgccga aagtgctctg gctcaggaac cacgagcctg cggccttcgc
     1621 ccgcctcgcc acggtcctcc tcgccaagga tttcgtacgg ctgcgcctga cggattcgct
     1681 cgcgaccgag ccgtcggatg cgtccgggac gctcatgttc gatctcgcag ccatgcgttg
     1741 gagcggtgac atcatggggg ctgtcggcct gcccgtgtcg ctgctgccgc aagtgggtgg
     1801 atcggcggag gtgctcggca gggtgaccgc tcaggcgagc acgctcaccg ggctccccac
     1861 gggcaccccg gtcgtcggcg gcggcgcgga caacgcctgt ggtgccgcgg gcgtgggcgt
     1921 cgtcacgccg ggggaggcgg tcgcgagctg ggggacctcc ggaacggtcc tcgcccctac
     1981 gcgagagccg cgtgtggacc ccgcgatgcg ggcgcacacc ttctgccatg tcgcgcccgg
     2041 cctgtggtac gtcataggcg tggtcctctc ggcgggaggg gcgttcgcgt ggtatcgcga
     2101 tcaactggcc cgcgacctcg ccggcaccgg tcaacgcgac gaccgcctgg tcgaggaggc
     2161 ggccgccgtg ccggcgggcg ccgagggggt cacgttcctg ccttacctgc agggggagcg
     2221 cacgccgcat cgggacgcct cgctccgcgg ggccttcctg gggttgagcc tcgcgcactc
     2281 gcgcgcgcac ctgacgcgcg ccgtgctcga gggcgtgtgc ttcgcgctgc gcgactcgct
     2341 cgtcattctc caggagctgg ggatggcgcc gcgctacctc ctgttgacgg gtggaggcgc
     2401 gaagagcgcg actatccgcc gcttgctggc ggacatcttc ggtctgccgg tgaccaccgt
     2461 gaaccgggag gagggcccgg cctacggcgc cgcgctgctc gcggcggtgg gagccggagc
     2521 gttccccgat ctcgcagcgg ccacgcagcg gacgctcgag cggtcgccgc tgatggaggc
     2581 cgatccggaa gcgcaccggc agtatgcgaa agtgtacgag cgcttccgaa actggtacgg
     2641 ggccgcaacg ttcagcgcgc gctgaacggc cgcctccatc cggtaccgca agccgggacg
     2701 cgcgtttcgt cactcggttc gctcgtcggg aatgcggatg tcgagaagat cctcgactgt
     2761 tctcaacatg acggacatgt ccgtctgtct acgatcgccg cctcgtcact gtggcgtgtc
     2821 caatggatgc cggctccgct cgtagaactc gttctgcagc cgctggctct cctggtccgc
     2881 cttgacggcg cggatcgccc ggacgaggcc ggagatccag gtccgcgcct cgtactggcc
     2941 cagctcacgc gaggccgcgg ccccttcgcc cgcatagtga ttggggaagc gcgcgtacca
     3001 ccagatgccg gtgtagaggc cttccggcaa gtgctggcgc gcctgatcgg cgcccgattc
     3061 ctgcgccgcg cggtcgaggt ggacgaggtc gggccgggcg atcatactca acgacgtttc
     3121 gctttccccg gcatgcatgt cggtcgctgg gttcgttttg tgcttcggct cgtccggccc
     3181 gccctccggc agaccctgca cgtacacgac gtagtcgtgc ggcgtgtcga gctgcgtctg
     3241 cgcgaagtag ggcagcaggc tgttgttgcc gccgtggccg ttcacgatga cgatcttctt
     3301 gcagccatta cgggccatct cgtcggtcgt ttcctgcaac aaatcgagct gcagccgccg
     3361 gctgtacgac accgtgcccg gctgatgctt cgcttcgaaa atctgcccga aatagtattc
     3421 cggaaacacg acggtgtatt cctgctgggc cgcgttgagc gccacgtagc ggacattgag
     3481 caggtcattg cccagcggca gatgcggccc gtgcttctcc atgatgccga aggggagcag
     3541 gcacgtaccc tgggctcggg tgatggcctc gcggaactcc gccgccgtca gctcctccca
     3601 gtgcaccgag agggtggctt gggtcgatcg gggcgattgg gccggtgccg atgacggtgt
     3661 ctgcgaccac aggatcgagg cgcagagtgc attgagggga agcagcaatg atttgtacac
     3721 gggtcctcct gctgcgcatg ggagccgatt ggagcggacc gacgcccgcg accagggcga
     3781 gcgggggggc gggaagccgc gagccgaagc cgaacggcag cgccgatccc ccaccgcaac
     3841 aagatgcctc ctgccgaggc gggaggcgaa tcgcgtagcg ctttcaagcc tcgcccctgg
     3901 acacccgcct cgagggtgac gaacatcggc cctccccctc cgaagcgctc agtgttgagc
     3961 caggaggtgg tccaattggt cgaagatcgg cttggaccag tcgcctctgg ccgtgacatt
     4021 cgtcgccaag cggcgtttcg tgaacacacg gatattccct tcttggcgat gatcatccag
     4081 gtgagcgagt ccggctgtga aaccgtatcc acgtcgagac ccgccgcgag gacggccggt
     4141 cacgtctcac ttcctcaaca gatagctcag cttgaggaag aatccgtccg tgacccgcga
     4201 cagtggccgg aaccggaacg gcgccccgtc gtcgagcctg ctcccgtacc ccacgaacac
     4261 caccgtgccg ggtgtgggcc ggtaggagaa cagccagtcg atccgcaggg cgttggtttc
     4321 gctcggagcg ctccgcgtga acgtccccgt caccgggtcg cgcaagagaa tggcgccgtt
     4381 ggtgcgtgca tcatcgcgca gcgcagcgac ctcgctcgcg gtgtattgcc cgatcagcct
     4441 gaagaagatt ggccgagtca gctggtactc gagccggagc tgcggcaccc ggatccgtgc
     4501 gacctggctg ccgtcgtcgc gccggttgac ctgctggtga ttgtagatca gctcggcgcg
     4561 gagccgatcg gtcggccgcc aggtgaggtc gacggtgccg agcacgacac gtcccgacgc
     4621 ccactcgtag aagtcttcat cgttgccgac gagcagaaac cccgagagcg agagcgattg
     4681 cacccgcggc gtctggaggt tcacgccgac gtccaggttg gcaatctgcg gctgcccgac
     4741 aaacggcact gtgtccaccg tcgccgcgtt cacgcgctcg acccagtagt tcgcgtagag
     4801 cggcgacggg tacccgaagg actcgacgga gacgcccatc cccacctgcc agccgccgcg
     4861 cagcgtaaac ccggcgctca tatcgtcctg tcgagtcgtc gggacgtgcc gggacgcgaa
     4921 ctgccggaac tgattccagg tcagggccag gtacgcattc gccgtgaagc tctcgatcaa
     4981 cgaaccggga acaccgacga tcgtgtaact cggtgtcacg ctggtggaca cgaagccggt
     5041 gcgcgagacg aacccgacgc ccgcgcggaa gtcgtctccc acggcggcgc tcctgagatc
     5101 gaggccaaaa cgtcgccccg agcggaccag gtccgcgcgc cacagcgggg cccagcgcac
     5161 gggagccgat gcggaatcgc gcgtcgcgct gccggcgccc tggaaccgga gcgcccacgc
     5221 gtcacccagg gtcaacgtgc cgtcgagggc tgcgacccgg ttgaccccgc ccccgtcgac
     5281 gcggtcggtg tagaccatcc ccaggttgct gtgcccgccc agatcccggc gcaggcgcag
     5341 cgcgttcatc accgggtgat cggtcccggt cgccgatgcg tcggtggcat ccacgccgga
     5401 gagcagcgcc accgtcgtcg ctcccacctt cccggtcagc ttgagcgcgg ccagcggatc
     5461 caccagccgt cgggtgtaga tcagctgcac cggtgtctgg aacagctcca ctccgtcgag
     5521 gaagaagggg cgcttctcgg ggaagttcag tgcgacgcgc ggatcaaact ggatctgcgg
     5581 cacgtcggcc tcgacctgtg agaagtcggg gtgcgcggtg gcgtcgaggg tgaggttcgc
     5641 agtcacgccc cagcgcaccg tggccccgat gcgcggggcg ccgccgctgt agtgccagcc
     5701 ctcaccccgc ggtcgaggcg ccccggtcac ggtgctcgtc acctcgggag tcgcatccaa
     5761 cacgaggccg cgtcgcaggg cggtgagccc ggtcagccga ccgctttgcg cgaggaacga
     5821 cgcctgtgtc tggagaacgc gggtccacgt gaactggtga ccggtggcct gaaccacccg
     5881 gatggcgttg agtccccaat cctgcggatc gcgcgtctgg tagcgcagcg tcttgaaggg
     5941 gatgcgcagc tcgacctcgt acccccaggc cgtctgccgg cccttcgatt ggtagacgta
     6001 gtcgggaccc agatccacgg tgtaggcccc cgctaccgcc gattgcgtga tatcgatcga
     6061 ctgccgcgcc gcatcgcgca gcgtgccgtc cgcctggacg ccgagcggat tgacgccgaa
     6121 gacgtacgcc tggcgttggt cgttgtaggt gtcgaggagg atctcgacgt agtcgtcgct
     6181 cgagatcttg tcgcggtccg ccagcgtggc gcgcaccaga gccgagtcct gataggcgcg
     6241 aatcccgaag tagagcgcct gcggcgcgta ccatacgagg acggtcgtcg agtcatcggc
     6301 tggacggtcg tcattgggga ggtactgcga gaacccacgc aggcgggcgg cgcgcgccca
     6361 cacgggctcg tcgagccgcc cgtctacgac gacggcggag tcggcggcgc ggggcggcgc
     6421 cacgggcgcc tggggggccg gggcgacggc ggcgaccgcg actccgagca acacgggcga
     6481 catggggtcc tcattctact aattgtttag tagaacttgg aggacctgca cggtcctgtc
     6541 aactacatat ctagttggtg cgtcccgccg gatggccgac gggaaacccg agcgcttgca
     6601 gggcgcagtg cagtccggcc ggctccggca ggaatgccgc ggcactgagg gcgaggacga
     6661 ccaccagcat ccgccatccg acgcggacgc gttccccacc gcgctcgcac atcgcgcgga
     6721 tccgtcgctc gagccacgag ggtggcgtgg acaaggccgg cgcggggagt ggagcgcccc
     6781 cgccccggct gccggcttcg agcagtacca ggccgtagct gtggagatcg gcgccacgat
     6841 cgagcacccg cgcgtcgcag tcgacttcga tcgcgaggcg cagacgaccg gcgagccacc
     6901 agaccgcggg gttccagggc tggagcagga cggcgacgag cggcagcaac agcacgcggg
     6961 tgtcgccgga ttgctggtgc tccagctcgt gggccagcat cagggcgcgc gcgcgatccc
     7021 ccgcgtcgag cgcccagcgt ggcaggacga tcgtgccgcg gccgatgccg aagacggcgg
     7081 ggccgtcggc gtccgagacg agcacggcgt gaccgtccac gatggcggcg cgccaggtgc
     7141 ggcgccgctg cagcaatcgc gcgaacgaca ggatgaacca cgcggcgaag agcgcggtcg
     7201 ctccccaggc cgcgatcgtg aggtgcgcat cccagcggtc gaaggcgtgg agagtctggg
     7261 cgaacgtgac cgccggcgcc ctcagccatg gggccgctgc ggcgcgtggc aggatgacgg
     7321 gcaggatccc gacttcccgc acgcgggcga tttcgctcac gagaacggat gccgcgaggg
     7381 cgagggccca cacccagcgc gtgggccggg cggcgtggcg gacgatccgc tcgagtggca
     7441 gtgccgccgc cgcggcgcag gcggcgagca acacgcagta gagcatccac ccgagcatca
     7501 cgaccgcgac tccttcagcc gctggtcgag gagttggcgc atgcgcttca actcgtcggc
     7561 ggagagccgc cgcccggaga cgagctgcgt cagcacgagc tccggggatc ccgagaagac
     7621 cttgtcggtg aggcggcgca gggcgctctt ccccgcggcg ttgcgtgcca cggtgggcgc
     7681 gtagcgatgg gccttccctg ccgcgcggtg gtcgacgtac cccttggatt ccagggtccg
     7741 gagcacggtg agcaccgtcg tgtaggcgag gtcgtcgtcg agccggtcgc ggacttcggt
     7801 caccgtggcc gatcccaggt cccagaggat cgccatcacg tcgagctcgc ggttggtgaa
     7861 cgtcaggtcc atggggatgc gacgagaagg aggatgctcg aacctagggc gcgggcggag
     7921 ggctgtcaac tattgaacta gtagacagtg ggaagggatg ccgtctgata cggatcgccc
     7981 ctaggctgcg gcttcggtct catcgtccgc gcacgacttg gctcgccacg cctcgacgcc
     8041 ctcgcggacg atcagcggca gcatcaccaa cgcggcgacc ggatccgccc accaccagcc
     8101 gagcagggcg ttcaggccca gaccgacgag cgtcgtaagc gagagccacg cacacacgat
     8161 cgtctcgtgg gcatcggccc gtagtgccgc gctctcgagc gcccgtgcgg tccggagctt
     8221 ggcgcgggcg aggagcggca tgacgatcag cgacagtgcc gtcagcgcca ctcccacggc
     8281 acttggctcg ggccggtccc ggacgatcaa tcgacgtccc gactcgacga cgagataggc
     8341 cgccaacgcc aggagcagcg ctccggcccc ccgagcggcc gcggcctccg ctctgggtcc
     8401 caccggtgca gagcccaatt ccgcgcgtag ccgccaccag aggacaacgc cgctggccac
     8461 ctcgatgacc gagtccactc cgaaaccggt gagcgccacc gagcccgcga cgagaccggc
     8521 ggcgatcgcc acgagccctt cgagcgcgtt gtaggtcacg gtcgcgactt cgagcagcac
     8581 gccgcggcgc aacagggaga cgcgcctcag gggctggtcg ggttcgagta tcgtcatggc
     8641 tggcgcctga acttactcac cgcgcggggc gcgacaagga agaagcccta cagcagaagt
     8701 cgctgcgtca aacgaggaca gcctgcaacg aggagatctc gcggcgccgc cgccggcgcg
     8761 cctacttcag accgagcttc ctgcccgccg caagcagcgc gatcgattcg cggatgcgcc
     8821 gctcgcgagt ctccggtcgc ttcgcgatgt ggatccagac gacgaagttg tgacgctccc
     8881 ttcgcggcag cttctgaaag aaactccacg cctcggggtt ggatttcatc gccttgccga
     8941 tgtacctcgg cagctcggga atgcgctgct tcggcgcgta tcggttctcg gtcggggcgg
     9001 ccgccaggcc cgccgccgtc agcaaccccg ccctcttcag ttccatccag cgccggcgat
     9061 tgatgtcgga ccatcggctc gtggctttgc gcggcgtcgc ctcgatcgcg aaccggtcgt
     9121 cgtcgagacg tttcacgagg ctgtcgatcc agccgaagcg gagcgcttcg cgaacgaagt
     9181 cctcgtagga catcgactgc acaccggtgt gcgccttgtg aaagacgacc cacattccgc
     9241 ggctggacgt gtggtgcttt ccgagccagg cgcgccattg tgcgcgagtt cggatgtcga
     9301 gaagcgctag ctcagccacg tcaggaaccg ttcaaagcga atccgcgtcg ccaccctgcc
     9361 cgcggtgttc atggacggcg cgccagcagt accaggcggc cacggtccga tacggccgaa
     9421 accgctcgcc ttcggcctgc agccgcgcag gcgtagggag cttgcgcaga ccgtaggcaa
     9481 gggcgtagcc caaccgcacc ccgtagtcgc cgaccggcca cacatccagc cggcgcagct
     9541 ggaagatcag gaacatctcc gccgtccagc gcccgatgcc tcgcaccact gagaggcgcg
     9601 tgatgatgtc ctcgtccgag cgccgggcga tgccccgcag cggcacggtt ccatccatca
     9661 ccttggcggc aaggtcgcgg agcgaggcca ccttgctctg cgagagcccg gcggcgcgca
     9721 gccgtctcgg atgcatggcc aggacagctt ccgcaaccgg tttcccgtcg aacagcgcga
     9781 tgaaccgccc gtggatcgcg gcggcccccg caccagcgag ctgctggtaa tggatgctct
     9841 cggcgagggc tgcgaagtgg gttctgcccg ttgggggaag ccggaagggg ccggtccgct
     9901 tcatgagacc ggccatgacg cgatcgcggc gggcgacggc cgcggccgcg atcgagaagg
     9961 tggtcttcca gttcggcgac atcctcccag tttaacgttg atccttcgtc tttgtctcat
    10021 gacgcgcgcc aagcggaaga cgcgctagct ccttcttcct ttctttgtca aggagcgcga
    10081 aggatctgct gtttgaagca aggaggcgcg gtgggtgagc cccggccttc gagctcgccg
    10141 gctacctgta ccgcccgctg cagagcacgc ccgaacgata tttcttccgc cgcggcgagc
    10201 cgcgccagta tcacctgcat ctcacgccgc tcgggagtca ggtctggcgc gaccagctca
    10261 ccttccgcga cctgctccgc ctcgatccgg tggtcgcatc ggcgtatgcg gcactgaagc
    10321 gcgagctggc cgcgcgc
//
LOCUS       DBNGZW010000696         3539 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_883102, whole genome shotgun sequence.
ACCESSION   DBNGZW010000696 DBNGZW010000000
VERSION     DBNGZW010000696.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3539)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3539)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3539
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_883102"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1699
                     /locus_tag="AC16JC_16435"
     CDS             <1..1699
                     /locus_tag="AC16JC_16435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR5166339.1"
                     /translation="AWLRYSQAEGIAEKPIEGDEIIYCSAGEGTTSEGEFWEALNTAS
                     NLKLPVLFLIEDNGYAISVPVEVQTAGGSVSALVANFPDLHIEEVDGCDPVASYEAIT
                     RAAAHCRARRGPALVHAHVTRPYSHSLSDDEVLYRPPSEREADSKRDCVVTFPECLKA
                     DGVATAAELEAIRQQVEEEVEVAAEVALAAHQPAPESALRFVYSPDVDPTSEQFDTED
                     DPHFSGEPTTMVDLLNTCMKDEMARDPRILVFGEDVADASRERYLKEVKGKGGVFKVT
                     LGLQRQFGSDRVYNSPLAEANIIGRAIGLATRGLRPVVEIQFFDYIWPGYHQLRNELA
                     TMRWRSNNAFSCPAVVRVTYGGYLKGGAVYHSQTGAVIFTAVPGLRVVCPSTALDANG
                     LLRTAIRCDDPVVFLEHKHLYRQTYNKAPNPGPNFMIPLGKARVAREGSHLSVVTYGA
                     VVQRALVAAKELEEQDGVSVEVIDLRSLAPVDWATIAASVQKTNKVIVAYEDSFSWGY
                     GAELAARLADECFPWLDAPVKRVAATDTFVAYSPDLEDFILPQVEDLAHAMRELHAF"
     gene            1689..>3539
                     /locus_tag="AC16JC_16440"
     CDS             1689..>3539
                     /locus_tag="AC16JC_16440"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HIR5166340.1"
                     /translation="MRSKAVLAYSLLFFLSGATGLVYELLWVRLLYQSFGSTIQSVTK
                     VVAAYMGGLGLGAWVFGRRADRHPRPAALYGRLEILIGLFGLISPLVLSLAQQAYLAI
                     ARGLPAGSGVSVMLRFGLAGAVLLVPTTLMGGTLPVLTRAFTSADRDRLRTYLGRLYG
                     INTLGAVLGTALAGFVLIEYVGIRASLLGTALVNLAIGFVALRFAAPFSSAPVPSAEP
                     GAQQRTSIGLRRAAVALLALTAFVSLADEIAWTRVLVMIVGGSTYAFTLVLLCFLLGI
                     GIGSALVARRGTVSRETAASAAYAQGLTAAGAALILVLLSVLPAYILWVFRVPGLDAV
                     PRLALMGGAVVAVVLIPAIGMGMTFPLLTDLVAAPDEARGADVGRAYLINTVGSIAGA
                     TLTGFVLVTALGSDVTLRGGILVNAVAALGLAGLAARDVKEDSPEHKRLRLRVVGGGI
                     LASAGLAAAIAAPRWSARLLDLGPTIYGRGAMTPAERRGFLSHAGARPLEFIEGRNTT
                     VSVWEMSAGRTLRVTGKVDASDYGDMDTQIMLGLAPVVARPHPHSALAIGFGSGVTTA
                     MLAATPGMDRVRVVELEPAVLALAPFFRHVNGDVLQRPNVRAIVDDARSAL"
BASE COUNT          508 a         1216 c         1199 g          616 t
ORIGIN      
        1 ggcgtggctg cgctactcgc aggcggaggg catcgccgag aagcccatcg agggcgacga
       61 gatcatctat tgctcggctg gggagggcac cacgagcgaa ggggaattct gggaagcgct
      121 caataccgcc agcaacctga agctccccgt cctctttctc atcgaggaca acgggtacgc
      181 gatttcggta ccggtcgagg tgcagaccgc gggcggatcg gtgtcggcac tcgtggccaa
      241 cttccccgac ctgcacatcg aggaagtaga cggctgcgac ccggtcgcgt cctatgaggc
      301 gatcacccgg gccgcggccc attgccgcgc ccggcgcggc cccgcgctcg tgcacgccca
      361 cgtcacgcgc ccctattcgc actccctctc ggatgacgag gtgctgtatc gccctccgtc
      421 agaacgggaa gccgactcga agcgcgactg tgtcgtcacc ttccccgagt gtctgaaggc
      481 cgatggggtg gcgacggcag cggagctgga ggcgatccgg caacaggtcg aggaagaggt
      541 cgaggtcgcg gccgaggtcg cgctcgccgc gcaccagccg gcgccggaga gcgccctccg
      601 cttcgtctac tcgccggacg tggatcccac ttcggagcag ttcgataccg aggacgaccc
      661 gcatttcagc ggcgagccca ccacgatggt ggacctgctg aacacctgca tgaaggacga
      721 gatggcgcgt gatccgcgca tcctcgtgtt cggcgaggac gtggccgacg ccagccggga
      781 gcggtacctg aaggaggtca agggaaaggg cggggtgttc aaggtcacct tgggactgca
      841 gcgccagttc ggcagcgacc gcgtgtacaa ctcacccctc gccgaagcga acatcatcgg
      901 ccgggccatt ggattggcca cgcgcggcct gaggccggtg gtcgagatcc agttcttcga
      961 ctacatctgg cccggctacc accaactgcg caacgagctg gccacgatgc gctggcggtc
     1021 caacaacgcc ttcagctgcc ccgccgtcgt tcgcgtgacg tacggcggat atctcaaagg
     1081 cggcgcggtc tatcactcgc agaccggcgc cgtgatcttc acggcggttc cggggctgcg
     1141 ggtcgtctgc ccgtccaccg cgctcgacgc caacggactg ctccgcaccg ccatccggtg
     1201 cgacgatccc gtggtgttcc tggagcacaa gcacctgtat cggcagacct acaacaaggc
     1261 gccgaacccc ggtcccaatt tcatgattcc gctcggcaag gcccgggtgg cacgcgaagg
     1321 ctcgcatctc agcgtcgtga cctacggtgc cgtcgtgcag cgggcgctgg tggccgcaaa
     1381 ggagctcgaa gagcaggacg gcgtgagcgt ggaggtgatc gatcttcgca gcctggctcc
     1441 ggtcgattgg gccaccatcg ccgcctccgt gcagaagacg aacaaggtga tcgtcgccta
     1501 tgaggattcg ttctcctggg gctacggggc ggagctcgcg gcacggctgg ccgacgagtg
     1561 ctttccctgg ctcgacgcgc ccgtgaagcg ggtcgcagcg accgatacgt tcgtcgcgta
     1621 ctctccggac ctcgaggact tcattctgcc acaagtcgag gacctcgcgc acgccatgcg
     1681 cgagctccat gcgttctaag gcagtcctcg cctacagcct gctgttcttc ttgtccggcg
     1741 ccacagggct cgtgtacgag ctcctgtggg tccggctact gtaccagtcc ttcggctcga
     1801 ccatccagtc ggtcaccaag gtggtcgcgg cgtacatggg gggcctcggg ctcggcgcct
     1861 gggtgttcgg gcgtcgggcc gaccgccacc cgcgtccggc tgcgttgtac gggcgtctcg
     1921 agatcctgat cggcctgttc ggcctcatct ccccgctcgt cctgtctctc gcccagcagg
     1981 cctacctcgc catcgcgcgc ggcctcccgg cggggtcagg ggtaagcgtc atgctgcgct
     2041 tcgggctcgc tggtgccgta ctcctcgtgc ccacgactct gatgggcggg accctgccgg
     2101 tgctcacgcg cgcgttcacg tccgccgatc gcgaccggtt gcgcacctat ctcgggcgct
     2161 tgtacgggat caatacgctg ggggccgtcc tggggacggc gctcgccggg ttcgtgctca
     2221 tcgagtacgt cggcatccgg gcgagcctcc tgggtacggc tctggtgaac ctcgccatcg
     2281 gcttcgtggc attgcggttc gcagcgccat tctcatccgc cccggtccct tccgcggagc
     2341 ctggtgccca gcagcgcacc tcgattgggt tgcgccgcgc cgcagtcgcg ctgctggcct
     2401 tgaccgcctt cgtgtctctg gcggacgaga tcgcctggac gcgggtgctg gtgatgatcg
     2461 tgggagggtc gacctatgcg ttcaccctcg tgctcctctg tttcctcctc gggatcggca
     2521 tcggtagcgc cctcgttgcc cgccgcggca cggtgtccag ggagacggcc gccagcgcgg
     2581 cctacgcgca aggtctgacc gccgccggcg ccgcactgat tctggtcctg ctctctgttc
     2641 tgccggccta catcctctgg gtgttccgcg tccctggtct cgacgccgtg ccgcgattgg
     2701 cgctgatggg aggcgccgtc gttgccgtcg tcctcatccc cgccatcggc atgggaatga
     2761 cgttcccgct gctgacggac ctcgtcgcgg cgccggatga agcccgcgga gcggatgtgg
     2821 ggcgggcgta cctgattaac acggtcggga gcatcgcggg cgcgacactc accggcttcg
     2881 tgctggtcac ggcgctcggg agcgacgtca cgctgcgcgg cggcattctc gtgaacgccg
     2941 tcgccgcgct cgggctcgcg ggcctggcgg cgcgcgatgt gaaggaggat tctccggagc
     3001 acaagcggct acgtcttcgc gtcgtcggcg ggggaatcct cgcgagcgcc ggactcgccg
     3061 ccgcgatcgc agctccgaga tggagcgccc ggctcctcga tcttggaccg accatctacg
     3121 ggcgcggcgc gatgacgccg gccgagcgcc ggggattcct gagccatgcc ggcgcccggc
     3181 cgctcgaatt catcgagggg cggaacacaa cggtttcggt gtgggagatg agcgcgggcc
     3241 ggaccctgcg ggtcaccgga aaggtggacg cgtccgacta cggcgacatg gacacccaga
     3301 tcatgctggg cctcgccccg gtcgtggccc gcccccatcc ccactcggcg ctcgccatcg
     3361 ggttcggcag cggcgtcacg acggcgatgc tcgccgccac gcccggcatg gaccgggtcc
     3421 gggtcgtgga gctcgaaccg gccgtgctgg ccctggcgcc cttctttcga cacgtcaacg
     3481 gggacgtgct ccaacggccc aacgtccggg cgatcgtcga tgacgctcgc agcgctctg
//
LOCUS       DBNGZW010000697         3588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_542645, whole genome shotgun sequence.
ACCESSION   DBNGZW010000697 DBNGZW010000000
VERSION     DBNGZW010000697.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3588
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_542645"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="AC16JC_16445"
     CDS             complement(<1..799)
                     /locus_tag="AC16JC_16445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015449190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5166341.1"
                     /translation="MKPRLRTIGLHGLLLVAATLAATPLLWMVSASFMQTGEANSSPP
                     PLLPHAPTLEHYRALFTSQSIGRYFANSVIISVGGTLLGVLITAMAGYGFAKLRFRGR
                     DGLFRILLAGMVVPGQVAMLPMFLELRQLGLINTLFGAMIPVLAPIYGVFLVRQYALS
                     IPDDLLDAARLDGAGELRIFRSVVLPTIAPVLVTLGVFTFLTAWNDFMWPLIVLSDDA
                     RYTLPVALANLAGEHVQDTEMMMASAVVTVLPVFLCFLFLQRYYIQGI"
     gene            complement(796..1671)
                     /locus_tag="AC16JC_16450"
     CDS             complement(796..1671)
                     /locus_tag="AC16JC_16450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019397693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR5166342.1"
                     /translation="MVEKRAGWAFVTPGLALIGVFFVIPVIGAFLLSFTDFDIYSIGD
                     PASTRFIGLDNYRTLFTTPLFWTALKNTAYYVVLGGPLSVVVSLAAALAVNSKAVRWK
                     PFFRSAFFAPWVTTLVAVALVWRYIYHPQYGLLNALLGGVGIGPIDWLGDPHWAMPAI
                     ILMSVWKNFGYNMLIFLAGLQTIPGELYEAADLDGAGSWGRFRHVTLPMLGPTMVFVG
                     VVTMIAAFQIFSEPYVMTQGGPLKSTLTLVLYMYEQGFRWWRLGFAAAVAMVLFLLTL
                     AGTLVQLWLRRERPA"
     gene            complement(1668..2924)
                     /locus_tag="AC16JC_16455"
     CDS             complement(1668..2924)
                     /locus_tag="AC16JC_16455"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR5166343.1"
                     /translation="MNRCALLALVAACAPRESPDTLRFWAFGREGEVVAQLVRDFERE
                     HPGVHVRVQQIPWTAAHEKLLTAHVGDATPDLAQMGNTWIPEMVTLGALAPLDSLVRA
                     SPGMDSTGHFPGIWATNVVDSRVYGIPWYVDTRVLFYRTDILARAGYPRMPDNWTDWL
                     AAMRAVTRVEGPGHYAIYLPLNEWPPQVLLGLEQGSPLITPDGHGAFADSGYARAFDF
                     LLSLYQQRLAPPATSNDIANLYQEFARGTFAMYITGPWNIGEFRRRLPPELQHSWTTA
                     PMPGPTGPESGVSLAGGSSLVILRSSKHQALAWELMRYLSRPEVQLQFYQLTGDLPAR
                     RETWTDSALAADREAQAFRIQLDRTVPTPMIPEWEEVTTKIMDQTEAAVRGGVTPAEA
                     LATLDRQVDRVLERRRYLLARAGTRK"
     gene            complement(2921..>3588)
                     /locus_tag="AC16JC_16460"
     CDS             complement(2921..>3588)
                     /locus_tag="AC16JC_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012930505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HIR5166344.1"
                     /translation="THIGFAPLFGHHYTHVWVDFRGIQDDYTRAHGLDYSENSRRATL
                     AQRAYALANPGGWTGYGADVWGLSASDGPADVTLPVNGSQRRFFTYAARGADFTEVRD
                     DGTIAPTAAGGSVPFAPEIAVPALIAMRERYGDWLFGTYGFLDAFNPTFRFSGQASAG
                     RIVAGQGWFDTDYLGIDEGPILAMIENYRSDLVWKTMRKSPYIVRGLRRAGFRGGWLD
                     QAP"
BASE COUNT          629 a         1183 c         1264 g          512 t
ORIGIN      
        1 tgatgccctg gatgtagtag cgctgcagga agaggaagca gaggaacacc ggcaacacgg
       61 tcacgaccgc gctcgccatc atcatttcgg tgtcctgcac atgctcgccc gcgaggttgg
      121 cgagcgccac ggggagcgtg tagcgcgcgt cgtcgctcag gacgatgagc ggccacatga
      181 agtcattcca ggcggtgagg aacgtgaata cgccaagggt gacgaggacg ggggcgatgg
      241 tgggcaggac gaccgagcgg aagatgcgca gctcgcccgc tccatcgagt cgcgccgcgt
      301 ccagcagatc gtcgggaatc gagagcgcgt actggcgcac cagaaacacg ccgtagatcg
      361 gcgccagcac cgggatcatc gcgccgaaca gcgtgttgat gaggccgagt tggcgcagct
      421 cgaggaacat cggcagcatc gcgacctggc ccggcaccac cataccggcc agcaggatgc
      481 ggaacagccc gtcgcggccg cgaaaccgga gcttggcgaa cccataaccc gccatggccg
      541 tgatcagcac gccgagcagc gtgccgccca ccgagatgat cacgctgttg gcgaagtagc
      601 gcccgatgct ctggctcgtg aacagggcgc gatagtgctc gagcgtgggc gcatgcggca
      661 gcagcggcgg gggcgacgag ttcgcctcgc ccgtctgcat gaacgacgcc gataccatcc
      721 acaggagcgg cgtggccgcc agcgtcgccg ccacgagcaa caatccatgc agaccgatcg
      781 tccggagccg gggcttcacg cgggtcgctc ccggcgcagc cacagctgga ccagtgtccc
      841 ggccagggtg agcaggaaca gcaccatcgc cacggcggcg gcgaatccca ggcgccacca
      901 gcggaacccc tgctcgtaca tgtagagcac cagggtgagc gtgctcttga gcggtccccc
      961 ctgcgtcatg acgtacggct cgctgaagat ctggaacgcc gcgatcatcg tgacgacgcc
     1021 cacgaacacc atcgtgggtc cgagcatggg aagcgtcacg tgccggaagc gcccccacga
     1081 cccggcgccg tccaggtcgg ccgcctcgta cagctcgccg gggatggtct gcagcccggc
     1141 gaggaagatc agcatgttgt agccgaagtt cttccacacc gacatgagga tgatggccgg
     1201 cattgcccag tgcgggtcgc cgagccagtc gatgggaccg atccccacgc cgccgagcag
     1261 cgcgttgagg agtccgtact gcggatggta gatgtagcgc cacaccaggg ccacggcgac
     1321 gagcgtggtg acccagggcg cgaagaacgc gctccggaag aacggcttcc atcgcacggc
     1381 cttggaattc accgcgagcg cggcggccag ggagacgacc accgacaacg gaccaccgag
     1441 gaccacgtag tacgccgtgt tcttgagcgc cgtccagaac agcggcgtcg tgaacaacgt
     1501 gcggtagttg tcgaggccga tgaaccgggt gctcgcggga tcgccgatcg aatagatgtc
     1561 gaagtcggtg aacgagagga ggaacgcccc gatcaccggg atgacgaaga acaccccgat
     1621 caacgccagg ccgggggtga cgaacgccca accggcgcgc ttctccacta tttccgggtc
     1681 cctgcccggg ccagcaggta gcgccgccgc tcgagcaccc ggtccacctg gcggtccagc
     1741 gtggccaggg cttcggccgg cgtgacgccg ccgcgcaccg cggcctcggt ctggtccatg
     1801 atcttggtcg tcacctcttc ccattccggg atcatgggtg tcggaactgt gcgatccaac
     1861 tggatccgaa atgcctgcgc ttcccggtcg gccgccaatg cggagtctgt ccaggtttcg
     1921 cgcctggcgg gcaggtcccc ggtgagctgg tagaactgca gctgcacctc agggcgcgag
     1981 agatagcgca tcagctccca cgccagcgcc tggtgtttcg agctgcggag gatcacaagg
     2041 ctcgaccctc cggcgaggga gacaccactc tccggtcccg tggggccggg catgggcgcg
     2101 gtggtccacg aatgctgcag ctcggggggc aggcggcgcc ggaattctcc gatgttccac
     2161 ggccccgtga tgtacatggc gaaggtgccg cgggcgaact cctgatacag gttggcgatg
     2221 tcgttgctcg tggccggcgg cgcgagccgc tgctggtaga gcgagaggag aaaatcgaag
     2281 gcgcgcgcgt atccggagtc cgcgaacgca ccgtggccat ccggggtgat gagcggcgag
     2341 ccttgctcga ggccgagcag cacctgcggg ggccattcgt tcaacggcag gtagatcgca
     2401 tagtgccccg ggccttccac gcgcgtgacg gcgcgcatcg ccgcgagcca gtcggtccag
     2461 ttgtcgggca tgcgggggta gccggcgcgc gccaggatgt cggtgcggta gaacagcacc
     2521 cgcgtgtcga cgtaccaggg aataccgtag accctggaat ccaccacgtt ggtcgcccag
     2581 atccccggga agtggccggt ggaatccatc cccggcgacg cgcgcacgag cgaatcgagc
     2641 ggcgcgagtg cgccgagcgt caccatttcg gggatccagg tgttgcccat ctgggcgagg
     2701 tcgggcgtgg cgtcgccgac gtgcgcggtg agcagcttct cgtgcgccgc ggtccagggg
     2761 atctgctgca cgcgcacgtg gacgcccggg tgctcgcgct cgaagtcgcg caccagctgc
     2821 gccaccacct caccctcgcg cccgaacgcc cagaaccgca gcgtgtcggg cgattcgcgc
     2881 ggcgcgcacg ccgcgacgag cgccagcagc gcgcaccggt tcacggcgcc tgatccagcc
     2941 atccgccgcg gaatccggcg cggcgcagcc cccggacgat gtagggactc ttgcgcatcg
     3001 tcttccagac gaggtcgctg cggtagttct cgatcatcgc gaggatcggt ccctcgtcga
     3061 tgccgaggta atcggtgtcg aaccagccct gccccgcgac gatcctgccg gcgctggctt
     3121 gccccgagaa ccgaaaggtg gggttgaagg cgtcgaggaa gccgtaggtg ccgaacagcc
     3181 agtcgccgta gcgctcccgc atggcgatca gcgccggcac ggcgatctcg ggcgcgaagg
     3241 gcactgagcc gcccgcggcc gtcggcgcga tggtgccgtc gtcgcgcact tcggtgaagt
     3301 cggcgccgcg cgcggcgtag gtgaagaagc ggcgctggct gccgttcacc gggagcgtca
     3361 cgtccgcggg accgtccgag gcggagagac cccacacgtc ggcgccgtag ccggtccagc
     3421 ctccggggtt cgccagcgcg taggcgcgct gcgccagcgt cgcccggcgc gagttctcgg
     3481 agtagtcgag cccgtgcgcg cgcgtatagt cgtcctggat gccgcggaaa tccacccaga
     3541 cgtgcgtgta gtggtggccg aacagcgggg cgaagccgat gtgcgtct
//
LOCUS       DBNGZW010000698         4315 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_65796, whole genome shotgun sequence.
ACCESSION   DBNGZW010000698 DBNGZW010000000
VERSION     DBNGZW010000698.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4315)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4315)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4315
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_65796"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1436
                     /locus_tag="AC16JC_16465"
     CDS             <1..1436
                     /locus_tag="AC16JC_16465"
                     /inference="COORDINATES: protein motif:HMM:NF025910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5166345.1"
                     /translation="QLPAAEGAFDRGDYRLARQLYDSVLRTDSLNPRALYRLAILDSW
                     DGKLDLALARFVKLRRVQPADPVTMAEHAKVLSWARLFRWSAALYDSVLVLAPDRLDA
                     QVGRARAVAWDGDLVRAERLWREALKAHPDDPDILIGLAQTLSWEGQTFLADAFATRA
                     REVAPNDPTARELFARLRAANRGPVLSLSSDAAGDKYDNGYVLFTGAFTASLRPDLRG
                     TLQAAWRYNEDGVPSGSARIARSGGLEGSIVKSLPSGASLRGGVGVRRLGGDTTRTRL
                     TAMLGGAVGVAQTTSINVAYRHYPFDETAALAQSGLVWDEVQANAEISPQATFGLSAL
                     ANAAWLSDGNRRLIGSGGVMVGVAKRLRAGVYARIMGWSRDDLGQNLYFSPGLFTLGE
                     ARASYVWWRGGWWTRATAGLGAQHIKSRATQGEWHGDLTVAYNWRYVDEVALVATYTN
                     SAEARTTTATTPTYRYWSVGLRYQRGF"
     gene            complement(1433..2641)
                     /locus_tag="AC16JC_16470"
     CDS             complement(1433..2641)
                     /locus_tag="AC16JC_16470"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HIR5166346.1"
                     /translation="MAARLTLVLLALPAVLAFRLQAQGRTAPRDYRAEALRILKAVPL
                     VDGHNDIPDAIRERGGVDSVDFAATQLGLMTDIPKLRAGGVGAQFWAAYVPTTTMHGG
                     EHPAAYALEQIDLVRRLCARYPRDLAMAYSAADVERNFKAGRISCLIGVEGGHAIESS
                     LGALRMFYALGVRYMTLTHWETTDWADAATDSAKHDGLTPFGEQVVREMNRLGMLVDL
                     SHVSDATMMDALHVSGAPVMFSHSSARAIANHVRNVPDSILKLVAANGGIVMVNFNPG
                     FVSEAVRVYEDSMQTRAKALRAAGADSTAVADSLKAWAKGAPHATLQQVADHVEHVRD
                     VAGVDHVGLGSDFDGISSVPDGLENTAKFPDLVAELLRRGWKEGDVRKLVGLNALRVL
                     REAGKAAGRR"
     gene            2713..3231
                     /locus_tag="AC16JC_16475"
     CDS             2713..3231
                     /locus_tag="AC16JC_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007715892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR5166347.1"
                     /translation="MADPHAQIVFELNGEPCTVGFAPHKTLLEVLREDLGLTGTKHGC
                     ELGECGTCTVLLDGQPVLSCLVLGLACDGRRVTTIEGMARGAELHPLQTAFADLGAAQ
                     CGYCTPAFLLAAQALLEQNPSPSRDEIKAALAGNLCRCTGYIKIYEAVELAAARLRGE
                     NADAPKESLYGR"
     gene            3284..>4315
                     /locus_tag="AC16JC_16480"
     CDS             3284..>4315
                     /locus_tag="AC16JC_16480"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR5166348.1"
                     /translation="MPSADSPTARPPERLTAGALRVVGKALRKVDAVAKVTGATKFAD
                     DLALPRMLYCKLLRSPHPHARIVRVDTGKAERLEGVKAVLTGKDFPIPFGILPVSQDE
                     HALCVDKVRFIGDPVAAVAATSEEAATAALDLIDVEYELLREIGSPADALAHPEPRIH
                     DYGDTGNLHKLINLEFGDVEEGFAAADVVREDLFFFEGNTHLPMEQHAALADWSADGK
                     VTLWSSTQTPHYVHRALAKVLELPAARIRVIATPNGGGFGGKSDPFSHEIVVAKFAML
                     TGRPVKITLTREEVFYCHRGRHPTLMWVKTGVTRDGAITGMHFRSLLDGGGYGSYGVA
                     STYYTGALQT"
BASE COUNT          670 a         1537 c         1465 g          643 t
ORIGIN      
        1 cccagctgcc ggcagccgag ggcgcattcg accgtggcga ctaccgcctc gcgcgccagc
       61 tgtacgactc ggtactccgg accgattcgc tcaacccgcg ggcgctctac cggttggcca
      121 tcctcgacag ctgggacggc aagctcgacc tggccctggc gcgcttcgtg aagctgcggc
      181 gcgttcagcc ggccgatccc gtcacgatgg cggagcacgc gaaggtgctg tcgtgggccc
      241 ggctcttccg ctggtccgcg gcgctgtacg attcggtgct ggtgctcgcc cccgaccggc
      301 tggatgcgca ggtggggcgt gcgcgcgccg tcgcctggga cggcgacctg gtccgtgccg
      361 agcggctatg gcgcgaggcg ctcaaagccc atcccgacga tcccgacatc ctgatcggcc
      421 tcgcccagac gctctcctgg gaaggccaga cgttcctcgc cgatgctttc gcgacccggg
      481 cccgcgaggt ggcgccgaac gatcccaccg cccgagagct cttcgcccgg ctgcgcgccg
      541 ccaaccgcgg ccccgtcctc tccctctcga gcgacgccgc cggcgacaag tacgacaacg
      601 gatacgtcct gttcaccggt gcgttcaccg cctcactccg tcccgatctg cgcgggacgt
      661 tgcaggctgc atggcgctac aacgaggacg gcgttccgag cggcagtgca cgtatcgcgc
      721 gcagcggcgg gctcgaaggc tcgatcgtga agtcgttacc gagcggggcg tcgctgcgcg
      781 gtggggtggg cgtgcggcgc ctgggcggcg acacgacgcg caccagactg accgccatgt
      841 tgggcggcgc cgtgggggtc gcccaaacga cctcgatcaa cgtggcgtac cggcattacc
      901 cgttcgacga gaccgcggcc ctcgcgcaga gcggcctcgt ctgggacgag gtccaagcga
      961 acgccgagat ctccccccag gcgacgttcg gcctgtcggc gctcgcaaac gcggcctggc
     1021 tctcggacgg gaaccggcgg ctcatcgggt ccggcggcgt catggtggga gtggcgaagc
     1081 ggctgcgggc cggcgtctac gcgcggatca tgggctggtc ccgcgacgac ctgggacaga
     1141 acctgtactt ctcccccggc ctgttcacgc tcggcgaggc ccgagcctcc tacgtctggt
     1201 ggcgcggcgg ctggtggacg cgggcgaccg ccgggctggg cgcgcagcac atcaaatcga
     1261 gagccacgca gggggaatgg cacggggacc tcaccgtcgc ctacaactgg cgctacgtgg
     1321 acgaggttgc cctcgtggcg acttacacca acagtgccga agcgcggacc acgacagcga
     1381 ccacgccgac ctatcgctac tggagcgtcg ggctgcgcta ccagcgggga ttctagcgcc
     1441 ggccggcggc cttccccgcc tcgcgcagta cccgcagggc gttcaggccc accagcttgc
     1501 ggacgtcgcc ctccttccag ccgcgccgca acagctctgc tacgagatcg gggaacttcg
     1561 cggtgttctc gagcccatcg ggaacgctcg agatgccgtc gaaatccgat cccaggccca
     1621 cgtggtccac cccggccacg tcgcgaacgt gctcgacgtg gtccgccacc tgctgcagcg
     1681 tggcgtgggg cgcgcctttc gcccacgcct tcagggagtc cgcgacggcc gtcgaatcgg
     1741 cgcccgcggc acgcagcgcc ttcgcccgcg tctgcatgct gtcctcgtac acccgcaccg
     1801 cttccgacac gaatcccggg ttgaagttga ccatcacgat gccgccgttc gcggccacga
     1861 gcttgaggat cgaatccggc acgttgcgca cgtggttcgc gatcgcgcgg gcggaggagt
     1921 gggagaacat caccggcgcc ccggagacgt gcagcgcgtc catcatcgtc gcgtcggaga
     1981 cgtgcgacag atccaccagc atcccgagcc ggttcatctc ccgaacgacc tgctcgccga
     2041 acggcgtgag cccgtcgtgc ttcgccgaat cggtcgcggc gtcggcccag tcggtggtct
     2101 cccagtgcgt cagcgtcatg tagcgcacgc ccagcgcata gaacatccgc agggccccga
     2161 gggagctttc gatcgcgtgc ccaccctcga ccccgatgag gcaggagatc cggcccgcct
     2221 tgaaattccg ctccacgtcg gcggcggagt aggccatggc gagatcgcgc ggatatctgg
     2281 cgcacagccg gcggacgaga tcgatctgct cgagcgcgta ggcggccggg tgctcacccc
     2341 cgtgcatcgt ggtcgtgggg acgtacgccg cccagaactg ggctccgacc ccgcccgcgc
     2401 gcagcttggg gatgtcggtc atgagaccga gctgcgtcgc cgcgaagtcc accgagtcca
     2461 cgccgccacg ttcgcggatc gcgtcgggaa tgtcgttgtg cccgtccacc agcggcacgg
     2521 ccttgagaat ccgcaacgcc tcggcccggt aatcgcgggg cgcggtccgt ccttgagcct
     2581 gcagccggaa cgcgaggacg gcgggtagcg cgaggagaac caaggtgagg cgcgcagcca
     2641 atggagcatc ctgtggaggt cgaacggaag ctggcgctgc gtcaccctga aggcaaggtt
     2701 agtttacgcg ccatggcgga cccgcacgcc cagatcgtgt tcgagctcaa cggcgagccc
     2761 tgcaccgtcg gcttcgctcc ccacaagacg ctgctcgaag tgctgcgcga agacctgggg
     2821 ctcaccggga cgaagcacgg ctgcgagctg ggtgaatgcg gcacctgcac cgtgctgctg
     2881 gacggccagc ccgtgctctc ctgtctcgtc ctcggcctgg cctgcgacgg ccggcgcgtc
     2941 accaccatag aaggaatggc gcgcggcgcc gagctgcatc cgctccagac cgccttcgcc
     3001 gacctcggcg ccgcccagtg cggctactgt acgccggcct ttctcctggc cgcccaggcg
     3061 ctcctcgagc agaacccgag cccctcgcgc gacgagatca aggcggcgct ggccggcaac
     3121 ctgtgccgct gcaccggcta catcaagatc tacgaggcgg tagagctcgc cgccgcacgg
     3181 ttgaggggtg agaacgcgga cgcccccaag gagtcgttgt atggccgata gggcggtcgg
     3241 gcggtcaggc ggtcgggcgg tcgcggatga gcgttcgggt accatgccgt ccgccgattc
     3301 tccgaccgcc cgaccgcctg aacgcctgac cgccggggcg ttacgggtgg tggggaaagc
     3361 actccgcaaa gtcgacgccg tcgcgaaagt caccggcgcg accaagttcg ccgacgacct
     3421 cgccctgccg cggatgctct actgcaagct gctgcgcagc ccgcatcccc acgcgcgcat
     3481 cgtccgggtc gacaccggca aggccgagcg gctggaaggc gtgaaggccg tcctcacggg
     3541 caaggatttc ccgattccct tcggcatcct ccccgtcagc caggacgagc acgccctctg
     3601 cgtggacaag gtgcgtttca tcggcgaccc ggtggcggcg gtcgccgcaa cgtcggagga
     3661 ggcggccacc gcggcgctcg acctgatcga cgtcgagtac gagctgctgc gcgagatcgg
     3721 ctcgcccgcc gacgcgctcg cccaccccga gccgcgcatc cacgattacg gcgataccgg
     3781 gaacctgcac aagctcatca atctggagtt cggggatgta gaggagggct tcgcggcggc
     3841 cgacgtggtg cgcgaggatc tgttcttctt cgaaggcaac acccacctcc ctatggagca
     3901 gcacgccgcg ctggccgact ggtcggccga cgggaaggtg acgctgtgga gctcgacgca
     3961 gacgccccac tacgtccacc gcgcgctcgc caaagtgctc gaactgccgg cggcgcgcat
     4021 ccgcgtcatc gccacgccca acggcggcgg gttcgggggc aagagcgacc cgttcagcca
     4081 cgagatcgtg gtcgccaagt tcgcgatgct caccggccgg ccggtgaaga tcaccctgac
     4141 ccgcgaggag gtcttctact gtcatcgcgg gcgccatccc acactcatgt gggtgaagac
     4201 cggggtcacg agagacggcg ccatcaccgg gatgcacttc cgctcgctgc tggacggcgg
     4261 cggctacggc tcctacggcg tcgcgagcac ttactacacg ggtgcgctgc agacc
//
LOCUS       DBNGZW010000699         2423 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_883384, whole genome shotgun sequence.
ACCESSION   DBNGZW010000699 DBNGZW010000000
VERSION     DBNGZW010000699.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_883384"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1301
                     /locus_tag="AC16JC_16485"
     CDS             <1..1301
                     /locus_tag="AC16JC_16485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR5166349.1"
                     /translation="SAVSFRGARLESAVAGEDRGGFVRALHRGHGWGIAAFTSLDELP
                     AMVRRATELSRAVRLDEPIRLAPVAAVTAEALLDLDGDVRGVPLAEKKRLLEGYNGEM
                     LAVPGVVDTQASYHDEVTDYWYANSEGTLLHELRPEITLSGTAIARRDGTIEKGLESI
                     GLRRGWRGAQDHAAGFGRAARRAAALLAAPTVKGGAYPVVLDNELAGVFIHEAFGHLS
                     EADFVYENPQAREMMTLGRRFGRSVLNVGDNGAAPGLRGTLPFDDEGTPTQDTPLIRD
                     GILVGRLHSRETAATLGERPTGNARAISFRHAPIVRMTNTYIANGTGSFADLIRDIKL
                     GVYACSAFGGQTMLENFSFTAAYGHMIRDGQVAELVKDVVLAGNLFQTLDRIERIAGD
                     FRWNQMGGGCGKGGQFPLPVTEGAPHVRIEQALVGGDVPA"
     gene            1298..>2423
                     /locus_tag="AC16JC_16490"
     CDS             1298..>2423
                     /locus_tag="AC16JC_16490"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF039907.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HIR5166350.1"
                     /translation="MIERVLELVRARSDAGDALWRRTERTSVTFESGRLKACAAAEET
                     GVNLRVLSGGRMGVAGTTAEDAEDLVTRAIASAALGETVPITFPAAAPLARVATASAP
                     AADAPLDRLTAIGTGLVDRVSREGCQVNVSIEREIAQTRVLNTAGADASFQTTAVVVS
                     LDLARFAGDDVLFVSDLYVGTGLPDAADLDALVRSIEDRYTPALRVVTPPDGSLPVVF
                     TPSGLSAILLPLEQALSGKTVLQGISPLGTKVGERLFDERFSLTDDPLAPGRPASRPV
                     DDEGTASVALPLIDRGVVRGFVYDLETAARAGATSTGHGARGTFGKPHVSYSNLILGT
                     RDEGRGTGGLGGGLLGGITDGLLIDDLIGVGQGNVIGGAFS"
BASE COUNT          333 a          809 c          926 g          355 t
ORIGIN      
        1 gctccgccgt gagcttccgg ggagcgcgcc tcgaatccgc cgtcgcgggc gaggatcgcg
       61 gcggcttcgt gcgtgcgttg caccgtggac acggctgggg catcgcggcc ttcacctcgc
      121 tcgacgaact gcccgcgatg gtgcgccgcg cgaccgagct gtcccgggcg gtgcgcctcg
      181 acgagccgat ccggctcgct ccggtggcgg cggtcaccgc cgaggcgctg ctcgacctgg
      241 atggcgacgt acgcggcgtg ccgctggcgg agaagaagcg tctgctcgag ggctacaacg
      301 gcgagatgct ggccgttccg ggcgtggtgg atacgcaggc gtcctaccac gacgaagtga
      361 ccgattactg gtacgccaat tccgagggga cgctgctgca cgagctgcgt cccgagatca
      421 cgctgtcggg cacggcgatc gcgcggcgcg acgggaccat cgagaagggg ctcgagtcga
      481 tcgggttgcg ccgcggctgg cgcggtgcgc aggaccacgc ggcggggttc ggccgggccg
      541 cacggcgcgc cgccgccctg ctcgctgcgc cgaccgtcaa agggggcgcc tatcccgtcg
      601 tgctcgacaa cgagctggcg ggggtgttca tccacgaagc cttcggccac ctctcggagg
      661 cggacttcgt gtacgagaac ccgcaggcgc gcgagatgat gacgctgggg cgccgcttcg
      721 gccggtccgt gctcaatgtg ggggacaacg gcgccgcgcc ggggctgcgc ggcaccctgc
      781 cgttcgacga cgagggcacg cccacccagg acacgcccct catccgcgac ggcatcctgg
      841 tgggccgact gcattcgcgt gagacggcgg cgaccctggg agagcgcccc accggcaacg
      901 cccgggcaat ctcgttccgc cacgcgccca tcgtgcgcat gacgaacacc tacatcgcga
      961 acggaacggg cagcttcgcg gacctgatcc gggacatcaa gctcggcgtc tacgcctgct
     1021 cggcgttcgg cggccagacg atgctcgaga atttctcctt cacggccgcg tacgggcaca
     1081 tgatccggga cggccaggta gcggagctgg tcaaggacgt ggtcctggcg ggtaacctgt
     1141 tccagacgct ggaccgcatc gagcggatcg cgggggattt ccgctggaac cagatgggcg
     1201 gggggtgcgg caagggcggg cagttcccgc tcccggtgac cgagggggcg ccgcacgtcc
     1261 ggatcgagca ggcgctggtc ggcggcgacg tgcccgcgtg atcgagcggg tgctggagct
     1321 ggtgcgggcc cgcagcgacg cgggggacgc gctctggcgc cggacggagc gtacgtcggt
     1381 gacgttcgaa tcgggacgtc tcaaggcctg tgcggcggcc gaggagaccg gcgtcaacct
     1441 gcgcgtgctg tcgggcggaa ggatgggcgt cgccggcacg acggcggagg atgccgagga
     1501 cctcgtcacg cgggcgatcg cctccgccgc cctgggcgag acggtgccca tcactttccc
     1561 ggccgccgcg ccgctggcgc gggtggcgac cgcgtcggcg ccggcagcgg acgcgccgct
     1621 cgaccgcctc accgcgatcg gcaccgggct cgtggaccgg gtgtcgcgcg aaggctgcca
     1681 ggtcaacgtg tcgatcgagc gcgagatcgc gcagacgcgg gtgctcaaca cggcgggcgc
     1741 tgacgcgtct ttccagacca ccgcggtcgt ggtgagtctg gatctggcgc ggttcgccgg
     1801 cgacgacgtg ctcttcgtct ccgacctgta cgtgggcacg gggttgccgg acgccgccga
     1861 tctcgacgcg ctggtgcgct cgatcgagga tcgttacacg ccggcgctgc gggtggtgac
     1921 gccgcccgac ggctccctcc cggtggtgtt cacgccgagc ggactctccg cgattctcct
     1981 gccgctcgag caggccctgt cgggcaagac cgtgctgcag gggatttccc cgctcgggac
     2041 gaaggtgggg gagcggttgt tcgacgagcg cttctccctc accgatgatc cactcgcgcc
     2101 cggccgcccg gcatcgcgcc ccgtggacga cgagggaacg gcgtcggtcg cgctgccgct
     2161 cattgaccgt ggcgtggtgc gcgggttcgt ctacgatctc gagaccgcgg cacgcgccgg
     2221 agcgaccagc accggccacg gggcccgcgg gaccttcgga aagccgcacg tctcgtattc
     2281 gaacctcatc ctggggacga gggacgaggg acgggggacg ggtgggctcg gcggtgggtt
     2341 gttgggtggg atcaccgacg gactgctcat cgatgacctg atcggggtgg ggcagggcaa
     2401 cgtgatcggc ggggcgttca gcc
//
LOCUS       DBNGZW010000700         2057 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1071792, whole genome shotgun sequence.
ACCESSION   DBNGZW010000700 DBNGZW010000000
VERSION     DBNGZW010000700.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1071792"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..952
                     /locus_tag="AC16JC_16495"
     CDS             <1..952
                     /locus_tag="AC16JC_16495"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR5166351.1"
                     /translation="PYLRVIMTTTSEATSRSTRLVGYACALGAGCLWGTTGPLSTALY
                     AQGATLTGIGFWRLVLGMVGILAYSATQHGFFRIDPRGWLLVGLGGGVLVALFEVAYQ
                     FAISGAGVAGAAALLYTAPVLVALLAVPLLGERLTSLRLILALVVMAGAGLAVLGGSR
                     AGVTVATTLRAGVKGGALAAISYAGTTLLARYAVPRYGPMRVLFLEILGGIVVLGVGL
                     TLAGHAPVPPPNSGAWIYTALLAAGTVVAANVLFFNAAKRIDAAPTAVAATIEPVVGA
                     LLAFALFAQRLTPLGWIGLLMVVGGVAGGYLEEAHPDTRS"
     gene            954..1619
                     /locus_tag="AC16JC_16500"
     CDS             954..1619
                     /locus_tag="AC16JC_16500"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012014219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR5166352.1"
                     /translation="MSALIDLSHVVTDGMVTYPGLPAPVIRDHLSREASVGRYAPGTE
                     FQIGRIEMVANTGTYVDAPFHRYAGGTDLAGLDLATLADLPGLVVRPGGSSPAIDHDV
                     FTTIDPRGRAVLIHTGWDRHWGTDAYGAGGHPFLTRAAAEHLARAGARLVGIDSLNID
                     DATDGTRPAHTVLLGAEIPIVEHLTGLERLPDDGFRFFAVPPKVMGMGSFPVRAFALV
                     SSA"
     gene            1633..2022
                     /locus_tag="AC16JC_16505"
     CDS             1633..2022
                     /locus_tag="AC16JC_16505"
                     /inference="COORDINATES: protein motif:HMM:NF021612.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HIR5166353.1"
                     /translation="MLDSVLAACLGAIIGLLIGFVYFLIWKARHTGIIRQDAVQRSQA
                     VTVGKVSEQLLPYLPGFRFNPKDARFLGSPVDLVVFDGLAEGDLRGIWFLEVKTGNAV
                     LSARERQIRDAILEKRVGWDELRLPRG"
BASE COUNT          255 a          742 c          707 g          353 t
ORIGIN      
        1 cccctacctt cgagtgatca tgaccacgac ctcggaggcg acttcccgct cgacgcgcct
       61 ggtgggctac gcctgcgcgc tgggcgcggg atgcctgtgg ggaacgaccg gcccgctctc
      121 gactgcactc tacgcgcaag gggcgaccct gaccgggatc ggcttttggc ggttggtcct
      181 gggcatggtg gggattctgg cgtacagcgc gacgcagcac ggcttctttc gcatcgaccc
      241 gcgcggctgg ctgctggtcg ggctcggggg cggagtcctg gtggccttgt tcgaggtcgc
      301 ctaccagttc gcgatctcgg gtgcgggcgt cgccggcgcg gccgcgttgc tgtacaccgc
      361 gcccgtcctg gtcgcgctgc tcgccgtacc gctgctgggt gagcgtctca cgtcgctccg
      421 tctgatcctc gcgctcgtcg tcatggccgg ggccggcctg gccgtgctgg gcggcagccg
      481 ggccggagtg acggtggcca cgaccctgcg tgcgggtgtc aaaggcggcg cgctggccgc
      541 gatatcctac gcgggcacga ccctcctcgc ccgctacgcc gtgccgcgct acggcccgat
      601 gcgcgtgctg tttctcgaaa tcctgggcgg gatcgtagtg ctcggcgtcg gcctgaccct
      661 cgccggccac gctcccgtgc cgccgcccaa ctccggcgcc tggatctaca cggcgctgct
      721 cgccgccggc accgtggtgg ccgcgaacgt gctgttcttc aacgccgcca agcggatcga
      781 cgcggccccg acggcggttg ccgccacgat cgagccggtg gtgggcgccc tgctcgcctt
      841 cgcgctcttc gcgcagcgcc tcacgccgct cggctggatc ggattgctca tggtggtcgg
      901 cggcgtggcc ggcggctacc tcgaggaggc ccaccccgac acgaggagtt gacgtgtctg
      961 cgctgatcga tctgagccac gtcgtgacgg acgggatggt gacctatccc gggctccccg
     1021 caccggtcat ccgcgaccat ctgagccggg aggcctcggt cgggcgctat gcgccgggga
     1081 ccgagttcca gatcggccgc atcgagatgg tggcgaacac gggcacctac gtggacgcgc
     1141 cgttccaccg ctatgccggc gggaccgatc tcgccggcct ggacctcgcg acgctcgcgg
     1201 acctcccggg gctggtcgtc cgcccgggcg gctcctcccc ggcgatcgac cacgatgtgt
     1261 tcaccacgat cgatccgcgc ggccgggcag tgctgatcca caccggctgg gaccgccact
     1321 ggggcacgga cgcgtacggc gccggcgggc atcccttcct cacgcgggcc gcggccgagc
     1381 atctggcccg tgccggtgca cggctcgtgg gcatcgactc gctgaacatc gacgacgcga
     1441 ccgacgggac ccgtcccgcg cacaccgtgc tgctcggcgc ggagatcccc atcgtggagc
     1501 atctgacggg gttggagcgg ctgcccgacg acgggttccg gttcttcgcg gtgccgccca
     1561 aggtaatggg aatgggctcg ttcccggtgc gcgcgttcgc gctcgtgtct tccgcctagc
     1621 atggagtggg acgtgctcga ttccgttctt gccgcgtgcc tcggcgccat catcgggctg
     1681 ctgatcggat tcgtctattt cctgatctgg aaggcgcgcc acaccggcat catccgccag
     1741 gacgccgtcc agcgcagcca ggccgtgact gtgggcaagg tctccgaaca gctcctgccc
     1801 tacctgcccg gcttccgctt caaccccaag gacgcgcgat tcctgggcag cccggtcgat
     1861 ctcgtggtgt tcgacggact cgccgaaggg gatctacgcg gcatctggtt cctggaggtg
     1921 aaaaccggca acgccgtact gagcgctcgc gagcgccaga tccgcgacgc catactcgaa
     1981 aagcgcgtcg ggtgggacga gctgcgcctg ccccgcggct gacgcctcct ccaccaacct
     2041 ccaccgtcct cccctag
//
LOCUS       DBNGZW010000701         8085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1700527, whole genome shotgun sequence.
ACCESSION   DBNGZW010000701 DBNGZW010000000
VERSION     DBNGZW010000701.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8085
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1700527"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..713
                     /locus_tag="AC16JC_16510"
     CDS             <1..713
                     /locus_tag="AC16JC_16510"
                     /inference="COORDINATES: protein motif:HMM:NF019307.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR5166354.1"
                     /translation="PNAHEELCRLLVRLKGPDGRINIPGLYAAVQAPSARERAAWKRL
                     PFREKDFLNRRVQAKALTGLTGHSVLERLWALPTFEIHGITGGFTGAGAKTAIPAEAV
                     AKVSLRLVPNQKMRTVERQLAAAVKKLAPKYVTATVRFLHGADPARVKIDHPAFKLLD
                     RAFHEVVGRGTVPARAGGSIPVVPALSREGAAVLLTGIGLPDDRLHAPDEKLDLQQLW
                     DGIRVFSRFYQLMGERRA"
     gene            915..1496
                     /locus_tag="AC16JC_16515"
     CDS             915..1496
                     /locus_tag="AC16JC_16515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166355.1"
                     /translation="MHRPLYLAGFGYLTVCAALVVRHSRHPGLPAPAPRTAPAAVRFD
                     GPADQWFAAIKPFCNEVEVEMAQRRHPAPQTLEGRGYSAACYALGGKLSAARTVIDAL
                     AEKDRYRAAGIVFGVAHPVADAGDDRSAGPIMELVVDYWPNNYMALYHAGMSEYALGQ
                     LGPARSHLQTFLTLYHQDDGWRRNALDVLQRLP"
     gene            1509..3329
                     /locus_tag="AC16JC_16520"
     CDS             1509..3329
                     /locus_tag="AC16JC_16520"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5166356.1"
                     /translation="MSESTYLPSGAVLQDRYEIDREIGRGGYSVVYQARDRRVGSDVA
                     IKLLVPPPAAARLARERLRREVQAVRRLSHPNVVTVYDVVDDGPWTFVVLEYVAGPDL
                     AVRVRQRGLLDPTAAARLGRDVAAALDAAHRAGILHRDVNPQNVLLAPDGRARLTDFG
                     SARLAGQATVTHTGGVVGTPAYAAPEVMAGVRGDARADCYGLGMTLYFGLVGELPSRG
                     ARDGGARVVARGGGHHPRERRADVPEWLDAAVARATAADPDERFPSVGLFAAALSPDA
                     EWEAEGGAAPRRAECAICGALEPFGLGVCPRCAGRSSTSDDVLIFVDRPIRPGTHGGV
                     LETLADRIGAETSAAGREAAARGERPLLRVPAEGSARIVQRLEAHGLAGRVESLPPPW
                     RATVPPAIAGLAGLVGVGGSLAGAFAGLPVLLLFSPLVAVGLVSAAAVGRRAPVWNPP
                     CGRPGSLPSDVEREAVRTAAALPEGPARSLLLDLLRRASATPAGSGSIGPLVGAACSA
                     ARELAALESHLESFDAQTERWADPPATWLDALTRCERGRDALVQRLLEAIAALSGASG
                     ERALRAASSGEALAILTRDLDEEGRLQAEAARDVEALLAR"
     gene            complement(3326..3940)
                     /locus_tag="AC16JC_16525"
     CDS             complement(3326..3940)
                     /locus_tag="AC16JC_16525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007720030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HIR5166357.1"
                     /translation="MTDRLPPGQILTKKWPVLHYGLVPAVDTATWAFEVSGLVDRPYT
                     ITWDELLALPRTTVRCDIHCVTTWSRFDNAFEGVAVRLLLERAGVSPAARFCLVHAEQ
                     DFTTNLPLADLDRPDNLIALKHDGDWLTPEHGGPARLLVPHLYFWKSAKWVRGFELLE
                     QDIPGFWEQNGYHMRGDPWTEERYGGRAITQHEINRFRNRSKRG"
     gene            complement(3937..4611)
                     /gene="lipB"
                     /locus_tag="AC16JC_16530"
     CDS             complement(3937..4611)
                     /gene="lipB"
                     /locus_tag="AC16JC_16530"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006981737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HIR5166358.1"
                     /translation="MKTLDVLMAGRVPYRAALAWQRALAEERIAGRRGDLLLLLEHDP
                     VVTLGRTSDSGNLLQPEGIDVVEVERGGDVTYHGPGQLVGYPILDLSGYRQDLHWYLR
                     TLEDALIVALARLDIPAGREPGRTGVWTSGRKIASIGVHVKRWVTWHGFALNVTTDLA
                     AFDRIVPCGIPGVIMTSIRQELGAGSRERSGTALWEETLHAVVAGFEETFQVSAQRVP
                     AEAILA"
     gene            complement(4608..4973)
                     /locus_tag="AC16JC_16535"
     CDS             complement(4608..4973)
                     /locus_tag="AC16JC_16535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166359.1"
                     /translation="MATLAQFVVALMRQHIPEVAYRDAAQDPLTFALVAPVAVGAFFG
                     WRRSHPIANIWQRGVIAVLAGLGGLLIGFMGAIADRIFGLAGLILWGIAAAAFGLAGS
                     RWAVRAAQPEARPESAGTP"
     gene            complement(5045..6436)
                     /gene="lpdA"
                     /locus_tag="AC16JC_16540"
     CDS             complement(5045..6436)
                     /gene="lpdA"
                     /locus_tag="AC16JC_16540"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR5166360.1"
                     /translation="MANQFDVIILGGGPAGYVCAIRAAQLGLATAVVEREKLGGVCVN
                     IGCIPTKALLHSAYIARLLGAATEFGVETGSVKTDYGVAMKRSRKVADQNSRGVDFLM
                     KKHKVAVFKGTGTLQPGRRVKVGSDVLEAKKAVVIATGSRVKGIPQIGLELNKTTVIS
                     SDEALVLEQAPASLAVIGAGAVGMEFADVFNAFGVPVTLIEALPRILPLEDAEASAEL
                     ARVYRKRGITVLAGANVTKATVAKDKVTLDVEAGGAKQQVEAAAVLMAAGRAVNTENI
                     GLKECGIQLTDRGFIKVDKNLQTSAAGYYAIGDVAGPPMLAHKGSREGVVVAELIAGQ
                     KPHPIKYDNVPSVTYCHPEVASIGLTEDQCKEKKLDYVAGKFPFSASGRARGTGEIEG
                     FVKILRDKQYGEILGAHIVGSHASELIHELAVARENEYTVEEVELAIHAHPTMSEAVA
                     EAALDSLGRVLNI"
     gene            complement(6541..7818)
                     /locus_tag="AC16JC_16545"
     CDS             complement(6541..7818)
                     /locus_tag="AC16JC_16545"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014067035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR5166361.1"
                     /translation="MATKVVMEALSPTMEEGRLVKWTKAVGDAVKSGETLAEVETDKA
                     VMELVARADGRLLQQAVKEGDTVPVGDLVAYIGAPGETVAATPVASPAKPTGSGERGA
                     VAVAATPAAVAQPAAVSRQPSAVSVAAAPVAVADATRVKASPLARRIARETGVDLRLL
                     HGSGPGGRVVRKDLEGAAAVPVPRPTSPVPLVVPGAAYEDVPLTQIRKTIAKRLATSL
                     GPVPHFFLTSEVDMERAAEAREALNRQLGDQGRDKISFNDIILKGVALALTRHRACNA
                     WWQDDHVRYWNEVHLGMAVAVEDGLITPVLRDADRRPLSEIGAVARELAQRARERRLK
                     PEEYTGSTFSVSNLGMFDIDEFTAVINPPEAGIVAVGAIVPKPVVVDGAVTVRRRLRL
                     TMSCDHRVIDGATGAAFLRTLKQMLENPLAMLL"
     gene            complement(7820..>8085)
                     /locus_tag="AC16JC_16550"
     CDS             complement(7820..>8085)
                     /locus_tag="AC16JC_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5166362.1"
                     /translation="PLDEAAILASVAKTHRAVVAEEGWPHSGIGAQVVDVIQRDAFDE
                     LDAPVLRVTQADAPMPYNKQLERLAKPTPDKIVAAARRVLYLD"
BASE COUNT         1087 a         2889 c         2839 g         1270 t
ORIGIN      
        1 cccccaacgc ccacgaggag ctgtgccggc tgctggtgcg tctcaagggc cccgacggcc
       61 ggatcaacat tccgggactg tacgccgccg tgcaggcgcc ctccgcccgc gagcgggcgg
      121 cctggaaacg gctcccgttt cgcgagaagg acttcctcaa tcgtcgcgtc caggcgaagg
      181 cgctgaccgg tctcacgggc cattccgtcc tggagcgcct ctgggcgctg ccgaccttcg
      241 agatccacgg cattaccggc gggttcacgg gcgccggcgc caagaccgcg attccggccg
      301 aagcggtcgc gaaggtgagc ctgcggctcg tgccgaacca gaagatgcgc accgtggagc
      361 ggcagctcgc cgcggccgtc aagaagctcg ccccgaagta cgtgaccgcc acggtgcggt
      421 tcctccacgg ggccgatccg gcccgggtga agatcgacca ccccgcgttc aagctgctcg
      481 accgggcgtt tcacgaggtg gtgggacggg gcaccgtccc cgcgcgcgcc ggcgggtcga
      541 tcccggtcgt gccggccctg agccgtgagg gcgccgccgt gctgctcacc ggcatcggcc
      601 ttcccgacga ccggctgcat gcgcccgacg agaaactcga cctgcagcag ctgtgggacg
      661 ggatccgggt gttcagccgc ttctatcagt tgatggggga acgccgggcc tagcaggtct
      721 gcgccgcgcc cttcctggct ggtcacggtg atcctggtcg tcgtggggat gctctggttc
      781 gccttgcggt tcttccgccg catgggcagc gccctgagag gctcgtaagc cctcgccgga
      841 cgggggcttc cccccgccgg cgcagcgtga ctcagtgcac atcggggcgg cgggggcgtc
      901 tctcatattg ccttatgcac cgtcccctct acctcgccgg gttcggctat ctcaccgtct
      961 gtgccgcgct ggtggtgcgc cattcccgcc accccgggct gcccgcgccc gccccccgga
     1021 ccgctccggc cgccgtgcgg ttcgacggcc ccgccgatca gtggttcgcg gcgatcaagc
     1081 cgttctgcaa cgaggtggag gtcgagatgg cccagcgccg gcacccggct ccacagacac
     1141 tcgagggtcg gggatacagc gcggcctgct acgcgctcgg agggaagctg tcggcggcac
     1201 gcacggtgat cgacgcgctg gcggagaagg atcgctaccg ggccgccggg atcgtcttcg
     1261 gcgtggccca tcccgtggcc gatgccgggg atgaccgctc ggcgggcccg atcatggagc
     1321 tggtggtcga ctactggccc aacaactaca tggccctgta tcacgccggg atgtccgagt
     1381 atgccctggg acagctcggg ccggcccgct cgcatctcca gacgttcctg actctgtatc
     1441 accaggacga cggctggcgg cggaacgcgc tggacgtcct gcagcgactt ccctgagcga
     1501 ccgccgtcgt gtctgagtcc acctacctgc cgtcgggcgc cgtcctgcag gaccgctacg
     1561 agatcgaccg ggagatcggg cgcggcggct attcggtcgt ctaccaggcg cgcgaccggc
     1621 gtgtggggag cgacgtcgcg atcaagctcc ttgtcccgcc gccggccgcg gcgcgactcg
     1681 cgcgcgagcg gctgcgccgg gaggtccagg ccgtccggcg actgagccac cccaatgtcg
     1741 tgacggtcta cgacgtcgtg gatgatgggc cctggacgtt cgtcgtgctc gagtacgtcg
     1801 cgggccccga tctcgcggtc cgggtgcgcc agcgcggcct gctcgatccc accgccgccg
     1861 cgcgcctcgg gcgggacgtg gcggccgcgc tcgacgccgc ccaccgtgcc ggcatcctgc
     1921 atcgcgacgt gaatccgcag aacgtgctgc tcgctcccga cgggcgggcg cggctcaccg
     1981 atttcggctc ggcgcgcctc gcgggccagg ccacggtcac ccacacgggt ggggtcgtgg
     2041 gcacgcccgc ctatgcggca ccggaggtca tggcgggcgt gaggggcgat gcccgggccg
     2101 actgctacgg gctcgggatg accctctact tcgggctggt gggagagttg ccgtcccgcg
     2161 gcgcgcggga cggaggcgcg agggtcgtgg cgaggggcgg gggccaccac ccgcgcgagc
     2221 ggcgcgcgga cgtgccggaa tggctcgatg cagccgtggc gcgggcgacc gcggccgacc
     2281 cggacgagcg gtttccgtcc gtgggcctgt tcgccgcggc cctgtctccc gacgcggaat
     2341 gggaagcgga agggggggcg gcgccccgcc gcgccgagtg cgcgatctgc ggcgccttgg
     2401 agccgttcgg actgggggtc tgtccgcgct gcgccggccg gtcgagcacc tccgacgacg
     2461 tgctgatctt cgtcgatcgc ccgatccgac cgggcacaca tggcggcgtg ctcgagaccc
     2521 tcgccgatcg catcggggcg gaaacgagcg cggccggccg ggaggcggcg gcgcgcggcg
     2581 agcggccgct gctgcgcgtt cccgccgagg gatcggcgcg gatcgtccag cgactcgagg
     2641 cgcatggcct ggccgggcgg gtcgagtcac tcccgccgcc gtggcgtgcg acggttcctc
     2701 ccgcgatcgc cggactcgcc ggtctggttg gcgtgggagg gtccctggcc ggcgcgttcg
     2761 cgggtctacc ggtcctgctg ctgttcagcc ccctcgtggc cgtggggctc gtgagcgcgg
     2821 cggccgtggg gcgccgggcg cccgtgtgga atccgccgtg cggcaggccg gggagcctgc
     2881 cgtccgacgt cgagcgcgag gcggtgcgca cggcggcggc gctgccggaa ggaccggcgc
     2941 ggagcctgtt gctggacctg ttgcggcgcg cctccgcgac cccagcgggg agcggatcga
     3001 tcggcccgct cgtgggcgcc gcctgctcgg cggcgcgtga gctggcggcg ctcgaatccc
     3061 atctcgagtc gttcgatgcg caaacggagc gctgggcgga tcctccggcg acgtggctcg
     3121 acgccctgac gcgctgcgag cgggggcgcg acgccctggt gcagcggctg ctcgaggcga
     3181 tcgccgcgtt gagcggggct tcgggcgagc gggcgttgcg ggcggcctcc tcgggcgagg
     3241 cgctcgcgat cctgacacgg gatctggatg aggaaggccg gctgcaggcg gaggcagcgc
     3301 gggacgtgga ggcgctgctc gcgcgctagc cgcgtttgct gcggttgcgg aaccggttga
     3361 tttcgtgctg cgtgatcgct cgcccgccgt agcgctcctc ggtccacggg tcgccgcgca
     3421 tgtggtaccc gttctgctcc cagaagccgg ggatgtcctg ctccagcagc tcgaagccgc
     3481 gcacccactt cgcgctcttc cagaaataca gatgcggcac gagcaaccgg gccggcccgc
     3541 cgtgctccgg cgtcagccag tcgccgtcgt gcttcagggc gatgagattg tcaggccggt
     3601 cgagatccgc gagcgggaga ttggtcgtga aatcctgctc cgcgtggacc aggcagaacc
     3661 gcgcggcagg gctcacgccg gcgcgctcca gcagcagccg caccgcgacg ccctcgaagg
     3721 cgttgtcgaa ccggctccag gtggtgacgc agtggatgtc gcagcgcacc gtggtgcggg
     3781 gcagggcgag cagctcgtcc cacgtgatcg tgtacggccg gtccacgagc cccgagactt
     3841 cgaaggccca tgtggcggta tccaccgccg ggacgagccc gtagtggagc acaggccact
     3901 tcttggtgag aatctggccg ggcggcagtc ggtccgtcac gcgaggatcg cctcggccgg
     3961 aacgcgctgc gcgctgacct ggaaggtttc ctcgaaaccg gcgacgacgg cgtgcagcgt
     4021 ttcctcccac agcgccgtcc ccgaccgctc cctgctcccc gctcccaatt cttgccggat
     4081 agaagtcatg atgactcccg gaatcccgca cgggacgatc cggtcgaacg cggcgaggtc
     4141 cgtcgtgacg ttcagggcga agccgtgcca ggtgacccag cgcttcacgt gcacgccgat
     4201 gctcgcgatc ttccgcccgc tcgtccacac gccggtgcgc ccgggctccc gtccggcggg
     4261 aatgtccagc cgcgccagcg cgacgatcag cgcgtcctcg agcgtacgca ggtaccagtg
     4321 caggtcctgc cggtatccgg agaggtcgag gatcgggtag ccgaccagct gcccggggcc
     4381 gtggtaggtg acgtcgccgc cgcgctccac ctcgacgacg tcgatgccct cgggctgcag
     4441 cagattgccg ctgtccgacg tgcggccgag cgtcaccacg ggatcgtgct ccaggaggag
     4501 cagcaggtcg ccgcgccggc cggcgatccg ctcctcggcg agcgcccgct gccacgcgag
     4561 agccgcccgg taggggacac gccccgccat caggacatcg agcgtcttca tggagttccg
     4621 gccgactcgg gtctcgcctc gggctgcgcg gcccgcaccg cccagcgact tccggccaac
     4681 ccgaacgccg ccgcagcgat gccccagagg atcaagcccg ccaggccgaa gatgcgatcc
     4741 gcgatggcgc ccatgaatcc gatgagcaag ccgcccaggc cagcgagcac ggcgatcacg
     4801 ccgcgctgcc agatgttcgc gattggatga ctgcgccgcc agccgaagaa cgcccccacc
     4861 gcgacgggcg caacgagcgc gaaagtcagc gggtcctggg ccgcatcccg gtaagcgact
     4921 tcggggatat gctggcgcat cagggctacc acgaattgcg cgagcgtcgc cacggctccg
     4981 cccaggccgg ccccggtggc gagggcgccg agaccgacgg cgagcgggtc tctcgcggcg
     5041 tcgctcagat attcagcacc cggccgagcg aatcgagcgc cgcctcggcc acggcctccg
     5101 acatcgtcgg atgggcgtgg atcgccagct cgacctcctc gacggtgtat tcgttctctc
     5161 gcgccaccgc gagctcgtgg atcaactccg aggcatgact gcccacgatg tgtgcaccca
     5221 ggatctcgcc gtactgcttg tcgcgcagga tcttcacgaa gccttcgatt tcgccggtgc
     5281 cgcgcgcccg tccgctggcg gaaaagggga acttgccggc cacgtagtcg agcttctttt
     5341 ccttgcactg gtcctcggtg agtccgatgc tcgccacctc ggggtggcag tacgtcacgc
     5401 ttggcacgtt gtcgtacttg atggggtggg gtttctgccc ggcgatcagc tcggccacga
     5461 cgacgccctc ccggctgccc ttgtgcgcca gcatcggcgg accggcgacg tcgccgatgg
     5521 cgtagtagcc ggcggcgctc gtctggaggt tcttgtccac cttgatgaac ccacggtccg
     5581 tcagctgaat cccgcattcc ttgagaccga tgttctcggt attcacggcc cggccggcgg
     5641 ccatcagcac cgccgcggct tccacttgtt gcttggcgcc tcccgcttcc acgtccagcg
     5701 tcaccttgtc cttggcgacg gtcgccttgg tgacgttcgc gccggcgagg accgtgatgc
     5761 cgcgcttgcg gtacaccctc gccagctcgg ccgacgcctc ggcgtcctcg agcggcagaa
     5821 tccgggggag cgcttcgatc agcgtgaccg ggaccccgaa ggcgttgaag acgtcggcga
     5881 actccatccc caccgcaccc gcaccgatga ccgcgagcga cgcgggcgcc tgctcgagca
     5941 ccagcgcctc gtccgaggag atcacggtcg tcttgttgag ctcgagcccg atttgcggga
     6001 tgcccttcac ccgcgatccg gtggcgatca ccacggcctt cttcgcctcc agcacgtcgc
     6061 tgccgacctt gacccggcgg ccgggctgca atgtcccggt ccccttgaag acggcgacct
     6121 tgtgcttctt catcaggaag tccacgccgc gggagttctg gtcggcgacc ttgcggctgc
     6181 gcttcatcgc gacgccgtag tcggtcttga cgctgccggt ttccacgccg aactccgtgg
     6241 ccgcgccgag cagccgggcg atatacgcgg agtgcagcag agccttcgtg ggaatgcagc
     6301 cgatgttgac gcacacgccg ccgagcttct cccgctccac caccgccgtg gcgagcccca
     6361 gctgcgcggc gcggatggcg cagacgtagc cggcgggccc accccccagg atgatgacat
     6421 cgaattgatt ggccactggt ctcctctgat tgcggatttc ggattgcgga ttgcggattg
     6481 ctcgcgaatc ggatcgcgac cagcgaatcc aatccgcaat ccgcaatccc caatccgcaa
     6541 ttacagcagc atcgccaacg ggttctccag catctgcttc agcgtcctga ggaacgcggc
     6601 tcccgtcgcg ccgtcgatca cgcggtggtc gcagctcatg gtgagtcgca ggcggcggcg
     6661 caccgtgacc gccccgtcca ccacgacggg cttcggcacg atggctccca cggccacgat
     6721 ccccgcttcg ggcgggttga tcacggcggt gaattcgtcg atgtcgaaca tgccgaggtt
     6781 cgagaccgag aaggtggagc cggtgtactc ctcgggcttg aggcggcgct cgcgcgcgcg
     6841 ctgggccagc tcccgggcga cggcgccgat ctccgacagc ggccgccggt cggcgtcgcg
     6901 cagcaccggc gtgatcaggc cgtcctctac cgccacggcc atcccgaggt ggacctcgtt
     6961 ccagtaacgg acatgatcgt cctgccacca ggcgttgcag gcgcgatgcc tggtgagggc
     7021 cagggcgacg cctttgagga tgatgtcgtt gaagctgatc ttgtccctgc cctggtcgcc
     7081 gagctgccgg ttgagcgcct cgcgcgcttc ggcggcccgc tccatgtcca cctcgctcgt
     7141 caggaagaag tgggggacgg gtccgagcga cgtcgcgagt cgcttcgcga tcgtctttcg
     7201 gatctgcgtc agcgggacgt cctcgtacgc ggcacccggg actaccaggg ggacggggga
     7261 cgtgggacga gggacgggta cggctgcagc gccttccagg tccttgcgaa cgacccgtcc
     7321 gcccggcccc gaaccatgca gcagtctcag atccacgccg gtttcgcggg cgatccgcct
     7381 cgcaagcgga gacgctttga cgcgcgtcgc atcggcgact gccaccgggg cagccgcgac
     7441 gctgacagcc gacggctgcc ggctgacggc tgccggctgg gcgaccgccg ccggcgttgc
     7501 cgcgacggcg acggctcccc gctccccgct ccccgtaggc ttcgcgggcg aggcgaccgg
     7561 cgtcgccgcg acggtctcgc ccggcgcccc gatgtacgcc accaggtccc ccaccggcac
     7621 cgtgtcgcct tccttgaccg cctgctgcag cagccggccg tcggcgcggg ccacgagctc
     7681 catcaccgcc ttgtccgtct ccacctcggc gagcgtctcg ccgctcttca cggcatcgcc
     7741 caccgccttg gtccacttga ccaggcggcc ctcctccatc gtgggcgaga gcgcctccat
     7801 cacgaccttg gtggccatgt cagtcgaggt agagaacccg gcgggcggcc gcgacgatct
     7861 tgtcgggcgt cggcttggcc aggcgctcga gctgcttgtt gtagggcatc ggcgcgtccg
     7921 cctgtgtcac ccgcagcacc ggggcatcca gctcgtcgaa cgcgtcgcgc tggatgacgt
     7981 ccaccacctg cgcaccgatg ccgctgtggg gccacccttc ctcggcgacg accgcccggt
     8041 gcgtcttcgc gaccgaggcg aggatggcgg cctcgtccag cggcc
//
LOCUS       DBNGZW010000702         3348 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_339029, whole genome shotgun sequence.
ACCESSION   DBNGZW010000702 DBNGZW010000000
VERSION     DBNGZW010000702.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_339029"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            28..3018
                     /locus_tag="AC16JC_16555"
     CDS             28..3018
                     /locus_tag="AC16JC_16555"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HIR5166363.1"
                     /translation="MLAKSIRLLALLSGICAAPAARAAAQQGSIAGRITDKVTQQPIQ
                     GAQVLIIGTNLQAVTNQEGRYRIDRISAGDYQVQVRLIGYAFGQQAVTVAAGDTLTLD
                     VALTAVAIPLEALVVTATGEEQRVKELGNAVTNIQASKITEVAPVNTVADLLNARAPG
                     VEVITSGGTTGSGSRIRIRGASSLSLTNEPIVVVDGVRVDNNPNSGLIGGLSTTGGQI
                     PSRLNDFNPADIQDIEVVKGPSAAALYGTDAANGVIQIRTKQGRPGPTRWTASAEGGL
                     LNDVGNWPANYRSLTSSGTSCTLEAAAAGACTIAALQSLNPIEQFSPFRQGARQQYSL
                     SASGGSEQTTFYTSGTFEREKGVFASNDLRSVSLRANIKNQVSRLMDISVSTGYVSSD
                     LQLPQNDNNDQGILSSGLLGTSDTSRNFCAQGHDCHGYGFLTPQEANQITAEQSIERF
                     TGSLNWNFRPADFLTLRAALGTDVTNRADNSTTPPSVIPLDQDRLDGGRLTARQQVFT
                     YTGNFSASANHRFSPRLTGTATVGVQYTKDVQSEIDASGRKLVGGTNSLGGVVVPTVG
                     EINLEFVTLGVFGQEQLNLNDRVFVTAALRGDDNSAFGANFNFITYPKLSASWVISDE
                     QFFPHPSWLSSLRLRGAWGRSGRQPGPTNALQFFTPVAVATNRADVPGITVGNLGNTD
                     LRPERTAETEVGLDADLLAQGRVHVEATYYAKSSRDALVARTLAPSLGVSATRFENLG
                     EVSNRGVELAVTAQVVRSAAFEWNLTASAWGNRNRLVHLGQGISPIIFGLGGASQRHE
                     EGYPLGGYWDFSYTYQDANGDGLISPDEITVASAQSFQGSTQPDHGGTLSSEFTVLRR
                     FRLYGLLDARYGNKLDNATENFRCLFAICRGDRDPSASLAEQARSVAIRNFGLETGFF
                     EDASFVKLREVSLTYSAPDTWARRVGASSMSFTVTGRNLVTWTNYTGIDPELNTAGQA
                     NFSTADFLTQPPVRYFTARVNLSF"
     gene            3035..>3348
                     /locus_tag="AC16JC_16560"
     CDS             3035..>3348
                     /locus_tag="AC16JC_16560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166364.1"
                     /translation="MRNPTQPQRLSLRLALVAAAVSLLPLAGCNDVLKVDNPDVVTPG
                     QLDDPATLPTVRAGAIGDFSLAYSGDGTNGTNTGTEGVVLYGGLLADEWVNSETFPTR
                     IEV"
BASE COUNT          569 a         1209 c         1076 g          494 t
ORIGIN      
        1 ctaccaatgc actgcccgag gaccactatg ctcgcgaaat ccattcgtct tctcgccctg
       61 ctgagcggca tctgcgccgc ccccgcggcc cgcgccgcgg cgcagcaggg ctccatcgcc
      121 ggccgcatca ccgacaaggt cacccagcag ccgattcagg gcgcgcaggt gctgatcatc
      181 ggcaccaacc tccaggcggt gaccaaccag gaaggccgct atcgcatcga tcggatctcc
      241 gcgggcgact accaggtgca agtccgtctg atcggctacg cgttcggcca gcaagcggtg
      301 accgtggccg cgggagacac gctcacgctg gacgtcgccc tcacggccgt cgcgatcccg
      361 ctcgaggcgc tggtggtcac cgccaccggc gaggagcagc gggtcaagga gctcggcaac
      421 gccgtcacca acatccaggc gtcgaagatc accgaggtag cgccggtgaa caccgtcgcc
      481 gatctcctca acgcccgcgc ccccggcgtg gaagtgatca ccagcggggg gacgaccgga
      541 tcgggctccc gcatccggat ccgcggcgcc agcagcctct cgctgaccaa cgagccgatc
      601 gtcgtggtgg acggcgtccg ggtggacaac aatcccaact ccggcctgat cggcggtctc
      661 tcgaccaccg gcggtcagat tccgtcccgc ctcaacgatt tcaatccggc cgacatccag
      721 gacatcgagg tcgtgaaggg cccctcggcg gcggcgctct acggcaccga cgcggccaac
      781 ggcgtcatcc agatccgcac caagcagggt cgccccggtc cgacgcgctg gaccgcctcg
      841 gcggagggcg gcctgctgaa cgacgtgggg aactggccgg cgaactatcg cagcctcacg
      901 tccagcggca cgtcgtgcac gctggaggcg gcggccgcgg gggcgtgcac catcgccgcg
      961 ctgcagagtc tcaacccgat cgagcagttc agcccgttcc gccagggggc ccggcagcag
     1021 tacagccttt cggcctcggg cggcagcgag cagacgacgt tctacacctc ggggacgttc
     1081 gagcgggaga agggtgtctt cgcctcgaac gacctgcgca gcgtgagcct gcgtgccaac
     1141 atcaaaaacc aggtcagccg gctgatggac atctcggtgt ccaccggata cgtgtcgagc
     1201 gatctgcagc tcccccagaa cgacaacaac gaccagggca ttctgtcgag cgggctgctc
     1261 gggacgtcgg acacctcccg gaacttctgc gcccagggac acgactgcca cggctacggt
     1321 ttcctcacgc cgcaggaggc caaccagatc accgccgagc agagcatcga gcgcttcacc
     1381 ggcagcctga actggaactt ccggccggcg gacttcctca cgctgcgcgc cgccctcggc
     1441 accgacgtga cgaaccgggc ggacaactcg accaccccac ccagcgtgat cccgctcgac
     1501 caggaccgcc tggatggcgg ccgcctgacg gcccggcaac aggtcttcac ctacaccggc
     1561 aacttctccg cctcggcgaa ccaccgcttc tcaccacgcc tcaccgggac cgccacggtc
     1621 ggcgtgcagt acacgaagga cgtccagagc gagatcgacg cctcgggccg gaaactggtc
     1681 ggcggcacga actcgctggg gggcgtggtc gttccgacgg tcggggagat caacctggaa
     1741 ttcgtcacgc tgggcgtctt cggccaggaa cagctcaacc tgaacgatcg ggtgttcgtg
     1801 accgccgccc tgcgcggcga cgacaattcg gcgttcggcg cgaacttcaa cttcatcacc
     1861 tatcccaagc tgtccgcctc gtgggtgatt tccgacgagc agttctttcc ccatccgtcg
     1921 tggctcagct cgttgcggct gcggggggcc tggggacgct cggggcggca gccggggccg
     1981 accaacgccc tgcagttctt cacgccggtc gcggtcgcca caaaccgggc ggacgtgccc
     2041 ggcatcacgg tcggcaacct cgggaacacc gacctgcgac ccgagcggac ggcggagacc
     2101 gaggtgggcc tggacgccga tctcctcgcg caggggcgcg tgcatgtcga ggcgacctac
     2161 tacgccaaga gctcccggga cgccctggtc gcccgcacac tggcgccgtc gctcggcgtc
     2221 agtgccaccc ggttcgagaa tctgggcgag gtgagcaacc gcggggtcga gctggcggtc
     2281 accgcccagg tcgtccggag cgccgccttc gaatggaatc tcaccgcctc ggcctggggc
     2341 aaccgcaacc ggctcgtcca cctgggtcag gggatctccc cgatcatctt cggcctgggc
     2401 ggcgcgtcgc agcgccacga ggaaggctat cccctcggcg ggtactggga cttctcctat
     2461 acctaccagg atgcgaacgg ggacggcctg atctcgcccg acgagatcac ggtggcttcg
     2521 gcgcagtcgt tccaggggtc gacccagccg gaccacggcg gcacgctctc gtccgagttc
     2581 acggtcctgc ggcgcttccg cctgtacggg ctgctcgacg cccggtacgg caacaagctg
     2641 gacaacgcca ccgaaaattt ccgctgcctg ttcgcgatct gccggggcga ccgcgatccg
     2701 tcggcgtccc tcgccgagca ggcccgctcg gtggcgatcc gcaacttcgg tctcgaaacc
     2761 ggattcttcg aggacgccag cttcgtcaag ttgcgggagg tctcgctgac ctactccgcg
     2821 ccggacacgt gggcgcgccg ggtgggggcg tcgagcatga gcttcaccgt cacgggccgc
     2881 aacctggtga cctggacgaa ctacaccggg atcgatcccg aattgaacac tgccggccag
     2941 gcgaatttca gcacggccga cttcctgact cagccgccgg tgcggtactt caccgcccgc
     3001 gtcaacctgt ccttctgagc cgaggcgatc atacatgagg aacccaacgc aaccccagag
     3061 gctgtcgctc cggctcgcgc tcgtcgcggc agccgtgtcg ctgctgccgc tggccggctg
     3121 caacgacgtc ctgaaggtgg acaatcccga cgtcgtaaca cccggacagc tggacgatcc
     3181 ggccacgctc cccaccgtgc gcgccggcgc catcggcgat ttctcgctgg cgtacagcgg
     3241 cgacggcacg aacggcacga acaccgggac cgagggcgtc gtcctgtacg gcgggctgct
     3301 cgccgacgag tgggtcaact ccgagacgtt cccgacccgc atcgaggt
//
LOCUS       DBNGZW010000703         2675 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1414006, whole genome shotgun sequence.
ACCESSION   DBNGZW010000703 DBNGZW010000000
VERSION     DBNGZW010000703.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1414006"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1770
                     /locus_tag="AC16JC_16565"
     CDS             <1..1770
                     /locus_tag="AC16JC_16565"
                     /inference="COORDINATES: protein motif:HMM:NF022214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 family protein"
                     /protein_id="HIR5166365.1"
                     /translation="VAAWGDSLFAVPARAPAELAAPLAAIRAADSTGEGRVLRALRFV
                     QEEVRYLGIEIGANSHRPYAPAVVMRRRYGDCKDKALLLITMLRELGITARPALVSTD
                     YGGHVADFAPTAQLFDHAIVRAEVDGRAVWVDPTALYERGDLQGAVPPFGAALVLGGP
                     ADSLSAIPQPPGVAPFTDVSVSFELHAVGTPAEMRVETRYSGGAANDVRASVRAKSAD
                     ELRRAYTDFYAEAYPSIRAVAPPEVHDDEGANVIRTIEQYSIPEFWHQSSDQDGYVGT
                     FDALELARVIPTTGAGARTMPLGITHPVHVRYRIEARLPQGWAIAARDRTIETPAMRF
                     VYHVKARDGVLALSYEYETRSDHVQPDAVADHLAKISQVRKLLAYTVTPPDTTATAST
                     WGDPDELNWTVLLVALLTTVAAGFAAVRLYRAPPPQWPRGPEGSEAAPKGLGGWLVLV
                     GIGVSITPLSVALSLVRTGPSYAASTWARLTTPGEGAYNAIWAPALLFELVANVTLLV
                     FGCLQLWTFFRRKRLFPALFVVFVCARLVVSVVDTLFAQTIPAVADRAALNWEGSLRT
                     LLYGVLWTAYMYRSRRVKNTFVE"
     gene            1776..>2675
                     /locus_tag="AC16JC_16570"
     CDS             1776..>2675
                     /locus_tag="AC16JC_16570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003619274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR5166366.1"
                     /translation="MRQSLRFVVSLLIPVPVRAQQPPSPPAFTFQEVMIPMRDGVRLQ
                     TVILTPANQTAPLPILFRRTPYGVPDGPLLQVPPTLRELAQDGYIFVIQNLRGRFKSE
                     GVFKLTSQVDLSDSTATNETTDAYDSIDWLVHNVPNTNGKVGMYGVSYDGLTTALALL
                     HPHPALRAVSEQAAPVDQWMNDDDHRYGALRESYAFEYAVLEQADKFKNTHFAFETYD
                     TYAWYLALGPLSNINDRYLHGSIPYWNDVVAHPDYDEFWKREAWISQLHASTVPNLNV
                     AGFWDQEDPWGPWQIFRHAAEHDP"
BASE COUNT          406 a          905 c          924 g          440 t
ORIGIN      
        1 gtggccgcgt ggggcgactc gctgttcgcg gttcctgcgc gtgcgccggc ggagctcgcg
       61 gctccgctcg ccgcgattcg cgccgcggat tcgacggggg aagggcgcgt gctgcgcgcg
      121 ctgcgcttcg tccaggagga agtgcgctac ctgggaatcg agatcggcgc caactcgcac
      181 cgcccctatg ctcccgccgt cgtgatgcgg cgtcgctatg gcgactgcaa ggacaaggcc
      241 ctgctgctga tcacgatgct gcgggagctg gggatcacgg cgcggcccgc gctggtgagc
      301 accgactatg gagggcacgt cgcggacttc gcgccgacgg cacagctgtt cgaccatgcg
      361 atcgtgcggg cggaggtcga cgggcgtgcg gtctgggtcg acccgaccgc gctgtacgag
      421 cgcggcgatc tccagggcgc tgtgcctccc ttcggcgccg cgctggtcct gggcgggccg
      481 gcggattcgc tgtccgcgat tccgcagccg ccgggcgtgg cgccgttcac ggacgtgtcc
      541 gtgtcgttcg agttgcacgc ggtggggacc ccggccgaga tgcgcgtgga aacacgctac
      601 agcggagggg ccgccaacga cgtccgcgcg tcggtgcggg cgaaatcggc ggacgagctg
      661 cggcgggcgt acaccgactt ctacgcggag gcgtatccct cgattcgcgc cgtggcgcct
      721 ccggaagtgc acgacgacga gggggccaac gtgatccgga cgatcgagca gtattcgatc
      781 ccggagttct ggcaccagtc gagcgatcag gacggctacg tggggacgtt cgacgcgctc
      841 gagctcgcga gggtcattcc gaccaccggc gccggggccc gcacgatgcc gctcggcatc
      901 acccatccgg tccacgtgcg ctaccgcatc gaggcgcggc tcccgcaagg gtgggcgatc
      961 gccgcgcgcg accggacgat cgagacgccg gccatgcgct tcgtctacca cgtcaaggcc
     1021 cgcgacggcg tactggccct gagttacgaa tacgaaaccc gctcggacca cgttcagccg
     1081 gacgccgttg cggatcatct ggcgaagatt tcccaggtgc ggaagctgct ggcgtacacg
     1141 gtgacgccgc ccgacactac ggcgaccgct tccacgtggg gggatccgga cgagctgaac
     1201 tggacggtcc tgctcgtcgc gctgctcacc acggtggcgg ccggcttcgc cgccgtccgc
     1261 ctgtaccgcg cgccgccgcc gcagtggccg cgcgggccgg agggttcgga ggcggcgccc
     1321 aaagggctcg gcggctggct cgtcctggtg gggatcggcg tgagcatcac cccgctttcc
     1381 gtagccctga gcctggtgcg gacggggccg agctacgcgg cttcgacgtg ggcccggctt
     1441 acgacgccgg gcgaaggcgc atacaatgca atctgggccc cggccttgct gttcgagctc
     1501 gtggccaatg tgacgctcct ggtctttggc tgcctgcagc tctggacgtt tttccgccgc
     1561 aagcgactgt ttcccgcatt gttcgtggtc ttcgtgtgcg ccaggctggt cgtcagcgtg
     1621 gtggatacgt tgttcgccca gacgatcccc gccgtcgccg atcgagcggc gctgaactgg
     1681 gaagggagcc tgaggaccct gttgtacggc gttctgtgga ccgcctacat gtaccgatcc
     1741 cgtcgggtca agaacacctt cgtcgagtag ggaccatgcg ccagagcctg cgcttcgtcg
     1801 tctcgctgct gatcccggtg ccggtgcggg cgcagcaacc gccgtcgcct cccgcgttca
     1861 ccttccaaga agtcatgatc ccgatgcgcg acggtgtgcg gctgcagacg gtgatcctga
     1921 cgccggccaa tcagaccgcg ccgctgccga tcctgttccg gcgcacgccg tacggcgtac
     1981 cggacggacc gctgctgcag gtgcccccga cgctgcgaga gctggcgcag gacggctaca
     2041 tcttcgtgat ccagaacctg cgcggccggt tcaagtccga gggggtcttc aagctgacct
     2101 cccaggtgga cctcagcgac tcgaccgcca ccaacgagac gaccgacgcc tacgacagca
     2161 tcgactggct ggtgcacaac gtgccgaaca ccaacggcaa ggtcgggatg tacggcgttt
     2221 cctacgacgg gttgacgacc gcgctggcgc tgctgcatcc ccatccggcg ctccgggcgg
     2281 tcagcgagca ggccgctccg gtcgatcagt ggatgaacga cgacgatcac cgctacggcg
     2341 cgctgcgcga gagctacgcc ttcgaatacg cggtgctgga gcaggcggac aagttcaaga
     2401 acacgcactt cgcgttcgag acctacgaca cctatgcgtg gtacctcgcg ctggggccgc
     2461 tctcgaacat caacgaccgc tacctccacg ggtcgatccc ctactggaac gatgtggtgg
     2521 cccatccgga ctacgacgag ttctggaaac gggaggcctg gatcagccag ctccatgcct
     2581 cgaccgtccc gaacctcaac gtcgccgggt tctgggatca ggaggacccg tgggggccct
     2641 ggcagatctt ccgccatgcc gcggaacacg atccc
//
LOCUS       DBNGZW010000704         2114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1072026, whole genome shotgun sequence.
ACCESSION   DBNGZW010000704 DBNGZW010000000
VERSION     DBNGZW010000704.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1072026"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..709
                     /locus_tag="AC16JC_16575"
     CDS             <1..709
                     /locus_tag="AC16JC_16575"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR5166367.1"
                     /translation="RPELGVREVARRLKLASSTAGRLLATLQKAGVLSQNAPMRKYTL
                     GPRVLAWAGIYTGILDVRAAAGPILDRLRQATRETTTLYVLDGDERVCVERLEGPQNI
                     RAVVRLGERLPLYAGAGGKVLLAFLPPGTRQTTLARLVLTRFTDKTIRNRDRLERQLR
                     TIRRQGYATSVAERFAGASAVAAPVRGADGAVIAAVNISGPSERFTGSRQVEYIRQVT
                     RAALALSRAMGGGRAHL"
     gene            729..1304
                     /locus_tag="AC16JC_16580"
     CDS             729..1304
                     /locus_tag="AC16JC_16580"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005288309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0106141 - flavin prenyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HIR5166368.1"
                     /translation="MQRLIVGITGASGAIYGIRLLEVLRGHGEIETHLVLTKPAERTI
                     AQETGYGVADVRAMASVVHTVADIGASIASGSFLTLGMVVAPCSIHTVAAIASCIADN
                     LLLRAADVVLKEGRKLVLLVRETPLHTGHLRCMLSAAEAGAVIMPPVPAFYHRPRTLE
                     QVVDHTVGRVLDRFGVAHELSPRWGEPPTPD"
     gene            complement(1318..>2114)
                     /locus_tag="AC16JC_16585"
     CDS             complement(1318..>2114)
                     /locus_tag="AC16JC_16585"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR5166369.1"
                     /translation="IRAASPAADAEAARTPHMIGQLLAEAAMTLLVTGGAVFLGGLVR
                     GATGFGGALVMVPLLARVTIPAAAVSLVLLIEVAGYADMLRHAGRRIQWRAIGVLTVA
                     AIVAVPLGVYAVTHVDTAIMTRVIAGCVIVLALILMTGWRYTGRPGWPVTVGVGAVSG
                     ALEGLAGVPGPPVALFLYGGPHAAQTVRDNLVGYFLLLDVATLVAFAIQGTLRAELLW
                     LALGSVPVSIVANWIGMWMARRLPENLLRRLSLLVVVAAGIALLLR"
BASE COUNT          339 a          761 c          731 g          283 t
ORIGIN      
        1 gcgccccgaa ctcggcgttc gcgaggtcgc ccggcgcctg aagctcgcga gcagcacggc
       61 ggggcgcctc ctcgcgacgc tgcagaaggc gggggtcctg tcgcagaacg ctccgatgcg
      121 gaagtacacc ctcgggcccc gtgtgctggc gtgggcagga atctacacgg gcatcctcga
      181 cgtgcgagcc gcagccggcc cgatcctcga ccgccttcgc caggccacgc gtgagacgac
      241 gaccctctac gtgctcgatg gcgacgagcg cgtgtgcgtc gagcgcctcg agggcccgca
      301 gaacatccgc gccgtggttc gcctcggtga gcggttaccg ctctatgcgg gcgccggcgg
      361 caaggtgttg ctcgccttcc tgccgccggg gacgcggcag acgacgctgg cgagactcgt
      421 gctgacgcgc ttcacggaca agacgatcag aaaccgggac cgacttgaac gccagctcag
      481 gacgatccgc cggcaggggt acgcgacgag cgtcgccgag cgatttgccg gcgcgtccgc
      541 ggtggcggcg ccggtgcgtg gcgccgatgg tgccgtgatc gcggctgtga acatcagcgg
      601 gccctcggaa cgattcaccg gtagtcggca ggtcgagtac atccgccaag tgaccagggc
      661 cgcgctcgcg ctctcccgcg cgatgggcgg cgggcgagca cacctgtgac gccagttccg
      721 gggggcctat gcagcgcttg atcgttggga tcaccggcgc ctcgggcgcc atctacggca
      781 tccggctgct cgaggtcctg cgcggacacg gggagatcga gacgcacctc gtgttgacga
      841 agccagcgga gcgcacgatc gcgcaggaga cgggctacgg cgtcgccgac gtgcgggcga
      901 tggcgagcgt cgtccacacc gtggcggaca tcggggccag catcgccagc ggctcgtttc
      961 tcacgctcgg catggtggtc gcgccctgct cgatccacac cgtcgcagcg atcgccagct
     1021 gcatcgccga caatctgctc ctccgcgcgg ccgacgtcgt cctgaaagag ggacggaagc
     1081 tggtgctgct cgtccgcgag acaccgctgc acacggggca tctccgctgc atgctctctg
     1141 ctgcggaagc cggcgccgtg atcatgccgc cggtcccggc gttctatcat cggccgcgga
     1201 cgctcgagca ggtcgttgat cacaccgtgg gccgcgtgct cgaccgcttc ggcgtcgcgc
     1261 acgagctgtc gccgcgctgg ggcgagccgc cgacgcctga ctgatccgcc ggccgactca
     1321 gcgcagaagc aacgctatcc cggcggccac caccaccagc agggacagcc tccgcaacag
     1381 gttctccggc aggcgccggg ccatccacat gccgatccag ttggccacga tggataccgg
     1441 cacggaaccc aatgccagcc acagcaattc cgcacgcagg gtcccttgga tggcgaaggc
     1501 gaccagggtc gccacgtcca gcagcaggaa gtaacccacc aggttgtccc gcaccgtctg
     1561 cgcggcgtgg gggccgccgt agaggaacag cgccaccggc ggacctggga cacctgccag
     1621 cccctccagc gcgccgctca ccgcgcccac gcccacggtc accggccagc cgggcctgcc
     1681 ggtgtagcgc cagccggtca tgagtatcag cgccaggacg atcacacacc cggcgatcac
     1741 ccgcgtcatg atggcagtgt ccacgtgggt taccgcgtac acccccagcg ggacggcgac
     1801 gatcgcggcg accgtgagca ccccgatggc ccgccactgg atgcggcgac ccgcgtgacg
     1861 cagcatgtcc gcgtatccgg cgacctcgat cagcagaacc agggagaccg cggcggcggg
     1921 gatcgtgacc cgggccagca gcggcaccat caccagcgct ccgccgaagc cggtcgcgcc
     1981 gcgcaccagg ccgcccagga acacggcgcc gccggtgacc agcagagtca tcgccgcttc
     2041 cgcgaggagt tgtccgatca tgtggggagt gcgagccgcc tcggcatcag ccgctggcga
     2101 cgcggctcgg atgg
//
LOCUS       DBNGZW010000705        12540 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1822188, whole genome shotgun sequence.
ACCESSION   DBNGZW010000705 DBNGZW010000000
VERSION     DBNGZW010000705.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 12540)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12540)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12540
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1822188"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="AC16JC_16590"
     CDS             complement(<1..379)
                     /locus_tag="AC16JC_16590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166370.1"
                     /translation="MSDPTHETGAPTPRRATIAETVASTDSFLRHAGREFLVLFYAAL
                     RSLKLYPLENAQVQKALDDLLTATRHLLDVEQELEVRLHGEFIFVNATRLRLALENYA
                     SFSHVLSVMRSCNVGAIRIEDQVD"
     gene            complement(376..2049)
                     /locus_tag="AC16JC_16595"
     CDS             complement(376..2049)
                     /locus_tag="AC16JC_16595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166371.1"
                     /translation="MTDVFGTELALPPAQVEELLSLIGKALRAVQLYLPNNPVYQRAI
                     DNVRVACRKIWEATGDLVLDVQESDLRWEGNVVYHHEQKNESIAWTLFKDGVRTVTLR
                     PGVEEQEIVRFMQVLHQARTLQADAADDLLTLLWQEDFQFLAYTFQEVQVDQPAPIEA
                     EENATTPAPVEVQQQVQQEAPPQREVVSADEFDTTLYFLEDQEIGYLQDEVEREYSQD
                     LRHNVLSLLFDLLELQTYGSVRAELIAIIENFIPYLLGSGDFHSVAFVLRQLREVVQR
                     AKELIPEHRETLLAIPTRLSQPEALSQLLQALDEATAHPSEEELGELFRELKPEAIVT
                     LAGWVSRLGSEQVRTLVKRAAERLAAQNPAEVLKALASSDTATALEMVRLAGRLRLAT
                     APEGMGALLQAGNREMKLAVVDALTSIASPIALRLLEGAVEDPERDVRIAAVRYLSSR
                     GHRGAFQRIETAVLGGKLRHVDLTEKIAFFEAFGALAGTAGVAALERILMPRGLLSRR
                     EDAASRACAAMALGKIRTPEARVVLERAVQDKEALVRNAVSRALREMPA"
     gene            complement(2046..3239)
                     /gene="trpB"
                     /locus_tag="AC16JC_16600"
     CDS             complement(2046..3239)
                     /gene="trpB"
                     /locus_tag="AC16JC_16600"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020505612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR5166372.1"
                     /translation="MTATREAAFGPYGGRYVPETLMGALDELAAVYDAARVDPGFWAE
                     LDRLQRDYVGRPTPLTEAVRFAAGLGPGVTVVLKREDLNHTGAHKINNTLGQALLARR
                     MGKRRIIAETGAGQHGVATATVCARFGLECVVYMGEEDMRRQRLNVYRMELLGARVVA
                     VTSGTRTLKDATNEAMRDWVAHVTDTHYIIGSVVGPDPFPRMVRDFQSVIGREARDQC
                     LDRFGKLPRTVVACVGGGSNAIGIFAAFLDDAAVELVGVEAAGEGIETGRHAASLSVG
                     RPGVFHGSRSYLLQDADGQVRPAHSISAGLDYPGVGPEHAFLKDQGRVLYQTVTDREA
                     LDAFTSLARLEGIVPALESAHAVAWVSRAKGRWQSGDLVLINLSGRGDKDVAMVADLP
                     GAGRP"
     gene            complement(3236..4036)
                     /gene="trpC"
                     /locus_tag="AC16JC_16605"
     CDS             complement(3236..4036)
                     /gene="trpC"
                     /locus_tag="AC16JC_16605"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007578315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR5166373.1"
                     /translation="MSHQLERILADTRERVARLEPRRGELEHRAANVSPPRFFGGLAP
                     DGSVGVIAEVKRRSPSAGAIHADLDPVAHARAYAAGGAVAISVLTEPEHFGGSLEDLQ
                     RVAGAVPVPVLCKDFILDEVQLLAARAAGASAVLLIARILTAIRLRELIAAASRLELG
                     VLVEVHSVDKLDGAIAAGAAVVGVNNRDLDSFEVDLAVGERVLRAVPRHLVAVAESGI
                     ESRADVERLAAAGADLVLVGTALVRHADPRTAVHALCGVPRQPRGMAA"
     gene            4099..4710
                     /gene="lexA"
                     /locus_tag="AC16JC_16610"
     CDS             4099..4710
                     /gene="lexA"
                     /locus_tag="AC16JC_16610"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HIR5166374.1"
                     /translation="MPLTRRQREILDYLTEYIGQRGYAPSFEEIAERFRFQSLATVHE
                     HLTNLERKGYIRRTPNESRAIEIVPPRGQTGATELPLLGLVAAGQPIETMSDANDTIA
                     VPDTLLPRRGRSYVLKVRGRSMIDEQIKDGDYIVVHERAQAENGETVVALVGGDSATV
                     KKYYREPGGWIRLQPANAEMTPIRVNERDVIVQGVVLGVIRRY"
     gene            complement(4694..5737)
                     /gene="trpD"
                     /locus_tag="AC16JC_16615"
     CDS             complement(4694..5737)
                     /gene="trpD"
                     /locus_tag="AC16JC_16615"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017925145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR5166375.1"
                     /translation="MTQRLTGPLERAILALAARESLGESLAAEVFGAVMRGEATPAQV
                     GGLLMGLRVKGETADEVAGAARALREAMVRVEIDAPHLVDTAGTGGGAVTTFNISTAA
                     ALVAAGSGATVAKHGNRSYTSRCGSADVFEALGVSVGLDASGAVRVLRQAGVAFLFAP
                     NFHPAMRHVAPVRRELGVTTLMNLLGPLANPAGVRRQVIGVADAPLGPLVANALCRLG
                     VEHALVVHGRVGMDEISPVGETSIWEVRDGDVSHWTLDPSDYGLAGHDVDALRGAEPA
                     ANAARLERLLEDDEDAAGRAAVLLNGAAAIYAAGLTTSYADGVERARAALDSGAARAA
                     LERLRRASPSTDG"
     gene            5846..6637
                     /gene="truA"
                     /locus_tag="AC16JC_16620"
     CDS             5846..6637
                     /gene="truA"
                     /locus_tag="AC16JC_16620"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR5166376.1"
                     /translation="MTSRPFLAVVQYDGGQFVGWQRQPAGRTVQAEFETVLARLMGGG
                     EPVTAIGAGRTDSGVHAVGQGVGFRGAVRWSADVDGLRRALNALLPRDIWVERVHAMR
                     DDFHARRSATARRYRYEIGTDDASHSPFRRPFEWALGQPLEQELLAAAARTLTGTHDF
                     RGLSAAGPARPDYRCRVAFAGWAPRADGTGVTFTIESDRFLHRMVRFLVGTMVDIALA
                     RRLPSDLSQLLAATDNQAASPPAPPQGLYLDAVRYPPDLYAEAHA"
     gene            6634..7302
                     /gene="fsa"
                     /locus_tag="AC16JC_16625"
     CDS             6634..7302
                     /gene="fsa"
                     /locus_tag="AC16JC_16625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006929619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HIR5166377.1"
                     /translation="MKFFLDTASLKDISWATQAGLIDGVTTNPSLLAKQAGDLDPRDV
                     LKEICSLVEGPVSAEVVAVEADAMVREGQSLARIADNIVVKIPMLEAGMVAVRRLAAA
                     GIRTNVTLCFSSVQCLIAAKAGATYVSPFIGRLDDVGQDGMDVIREARQIFDNYTLAT
                     QILAASMRHPRHVVEAAMIGADVATLPPDVLRKLLQHPLTDRGLEQFLADWQSLAARA
                     KASV"
     gene            7299..8732
                     /gene="purB"
                     /locus_tag="AC16JC_16630"
     CDS             7299..8732
                     /gene="purB"
                     /locus_tag="AC16JC_16630"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013563541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR5166378.1"
                     /translation="MTWESPLSSRYASPAMQALWSERRRIGLWRRLWLALMESERELG
                     LEVPAEAIAQLRGHLDDVDLPRAAEYEKRMRHDVMAHIHALGEQAPAARPYLHLGATS
                     AFVTDNADLILMREGLGLLLGRVAAVLVALSHTARRHRALPCLAYTHFQPAQLTTVGK
                     RATLWMQEFLLDAEELLHRGEALQFRGVKGTTGTQASFLELFDGDHDKVLELDVRVAA
                     KLGFSRVFPVTGQTYTRKQDAQVLAALAGIAASAAKFATDLRLLQHEGELLEPFESEQ
                     VGSSAMPYKRNPMRAERMTSLARFVIELEGNAWHTAAEQWLERTLDDSANRRLVIPEA
                     FLATDAILVLATNVAAGLEVREAVIRRHVEDALPYLATERLLMRAVKAGGDRQRLHEV
                     IRVHSLAVSQGVAERGATNDLLARLGSDPAFRALGIAAHPHELDPAQYVGRAPEQVDH
                     FLDEVLPAVLHRIDAVVPAAGTAEVQV"
     gene            8729..9658
                     /locus_tag="AC16JC_16635"
     CDS             8729..9658
                     /locus_tag="AC16JC_16635"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HIR5166379.1"
                     /translation="MTTVTPLVASHLPLPLLRRGKVREMYEVDGDTLLMVASDRVSAF
                     DVVLREPVAFKGAVLTQLSAFWFRRLAHVLPNHLVTADAGEIVARVRTLAPHRGTLQG
                     RAMLVRRTTPVAFECVVRGYLAGSAWAEYKRSGTLAGEALPSGLVESARLEPAIFSPA
                     TKAETGHDENVTFAHMAQALGGPLADALKRASLALYEEGRAYAAGRGIIIADTKFEFG
                     TTADGTLLLIDEVLTPDSSRFWPADRYQPGTGQPSFDKQPLRDFLSGLKEQGKWDGND
                     APPALPPVVIAATSARYREAYRLITGASLEVTA"
     gene            9661..10308
                     /locus_tag="AC16JC_16640"
     CDS             9661..10308
                     /locus_tag="AC16JC_16640"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007881896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HIR5166380.1"
                     /translation="MAPEGWPFIALGWGVAALGAWATLRWGGWWWVIESVVLLLAVWL
                     LVFFRNPERTGPRGDRYVIAPADGKIVSTVETDEPAYLRTRALRISIFMSVFDVHVNR
                     YPVSGTVAYRHYNAGQFLHAAADKASLDNEQAAIGIEGPHGPVLVRQIAGLIARRIVT
                     DATVGDRAAQGARLGMIRFGSRVDVYLPLTARPASRVQVGDRVAAGATVLAEYGP"
     gene            10305..11168
                     /gene="pssA"
                     /locus_tag="AC16JC_16645"
     CDS             10305..11168
                     /gene="pssA"
                     /locus_tag="AC16JC_16645"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR5166381.1"
                     /translation="MTATPPKARRGVRRVVVIVPSLFTLFNLFFGIWSITLAAKGEFY
                     RASWFLVFAGILDTLDGRVARLSNTGTRFGAELDSLVDVVSFGVAPAYLMYELHFATA
                     GPAAWIFCYFYIMGAAIRLARFNITQAGRAKAHFVGLPSPAAGLTLATYYPFTQTDLY
                     AQMRTLPWPLLLNFLMIALTILMVSNVRYATLPRASLRSWRGLLGLLTLLCIVFFGIW
                     QHDAFFFPLGIVYMVYGVARAALLGFFENGDDSGETDIAGPIVITDASADRVDDAFGP
                     RSRGISPRGRH"
     gene            11168..11422
                     /gene="purS"
                     /locus_tag="AC16JC_16650"
     CDS             11168..11422
                     /gene="purS"
                     /locus_tag="AC16JC_16650"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HIR5166382.1"
                     /translation="MPAFRVEIRVMPRAALLDPQGQAVSHALHALGFADVGDVRVGKH
                     LVVRLEASTSDEAAARARAMCDRLLANPVTEDYQLLVEAA"
     gene            11422..12138
                     /gene="purQ"
                     /locus_tag="AC16JC_16655"
     CDS             11422..12138
                     /gene="purQ"
                     /locus_tag="AC16JC_16655"
                     /EC_number="3.5.1.2"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR5166383.1"
                     /translation="MHRVAVVVFPGSNCETETVDASRAAGSDAYEVWHRETDLRQADA
                     VILPGGFSYGDYLRSGAIARFSPVMAAVAAHARAGGAVLGICNGFQILCEAGLLPGGL
                     MRNVGLRFLSRPVRVRVEANDTPWTRGFVPGEEIVAPVASGDGRYVADERTAAELEAE
                     GRIVWRYVGENPNGSTRAIAGVSNASCNVVGIMPHPERRNASVLGGTDGARVFLSLEA
                     DHATQHAGGVRAVGGHHAGL"
BASE COUNT         1819 a         4554 c         4268 g         1899 t
ORIGIN      
        1 gatccacctg atcttcgatg cggatggccc cgacgttgca gctgcgcatc acagacagca
       61 cgtgtgagaa ggaggcgtag ttctcgagcg ccaggcgcag gcgcgtggcg ttcacgaaga
      121 tgaactcccc gtggagccgc acctccagct cctgctccac gtccagtagg tggcgcgtgg
      181 cggtcagcag gtcgtccagc gccttttgca cctgcgcatt ctcgagcggg tacagcttca
      241 gcgaccggag cgcggcgtag aagagcacca ggaactcccg gcccgcgtgc ctcaggaagc
      301 tgtcggtcga ggcgaccgtc tccgcgatgg tggcgcggcg cggcgtcggt gcgccggtct
      361 cgtgggtggg gtcgctcatg ccggcatctc ccgcagcgcc cggctcacgg cgttgcggac
      421 cagcgcttcc ttgtcctgga cggcgcgctc cagcaccacc cgggcctccg gtgtgcggat
      481 cttccccagt gccatcgccg cgcaggcccg gctcgcggca tcctcccgtc gcgacagcaa
      541 cccccgcggc atcaggatgc gctccagtgc ggccaccccc gccgtgccgg ccagagcgcc
      601 gaacgcctcg aagaaggcga tcttctcggt gagatccacg tgacgcagct tgccgccgag
      661 gaccgccgtc tcgatgcgct ggaacgcacc gcgatggccg cggctgctga ggtatcgtac
      721 ggccgcgatc cggacgtcac gctccggatc ttccacggcg ccctccagca atcggagcgc
      781 aatggggctg gcgatcgacg tgagcgcgtc caccaccgcc agcttcatct cgcggttccc
      841 ggcctgcagc agcgccccca tgccttcggg cgcggtcgcc agccgcagcc ggcccgccag
      901 ccgtaccatc tcgagcgccg tcgccgtgtc ggagctggcg agcgccttca acacctccgc
      961 cggattctgg gccgcgagcc gttcggccgc ccgcttgacc agcgtccgca cctgctcgct
     1021 gccgagccgg gacacccagc ccgccagcgt cacgatcgct tccggcttga gctcccggaa
     1081 caactcgccc agttcctctt cggacgggtg cgcggtcgct tcgtcgagcg cctgcagcag
     1141 ctgcgagagc gcctcgggct gggacagacg cgtcgggatc gcgagcagcg tctcgcggtg
     1201 ctcgggaatg agctccttgg cccgctggac cacctcgcgc agctgacgca gcacgaacgc
     1261 taccgaatga aagtcccccg agccgagcag atacgggatg aagttctcga tgatggcgat
     1321 cagctcggcg cggaccgacc cgtacgtctg gagctccagc aggtcgaaca acagggagag
     1381 cacgttgtgg cgcaggtcct gcgaatactc ccgctcgacc tcgtcctgca ggtagccgat
     1441 ctcctggtcc tcaaggaagt acagggtggt gtcgaattcg tcggccgaca cgacctcccg
     1501 ttggggcggt gcctcctgct ggacctgctg ctgcacctcg accggcgccg gcgtggtggc
     1561 gttctcctcg gcctcgatcg gcgcgggctg gtcgacctgg acttcctgaa aggtgtaggc
     1621 gagaaactgg aaatcctcct gccacagcag cgtcagcagg tcgtcggccg catcggcctg
     1681 gagggtgcgc gcctgatgca gcacctgcat gaagcgcacg atctcctgct cctccactcc
     1741 cggccgcaag gtgaccgtcc ggaccccgtc cttgaacagg gtccacgcga tgctctcgtt
     1801 cttctgctcg tggtggtaga cgacgttccc ttcccagcgg aggtcgcttt cctgcacgtc
     1861 gagcaccagg tcgccggtcg cctcccagat cttgcggcag gccacccgga cgttgtcgat
     1921 ggcgcgctgg tagaccgggt tgttggggag gtacagctgc acggcgcgca gggccttgcc
     1981 gatgagcgac agcagctcct cgacctgggc cggcgggagc gcgagctcgg tgccgaagac
     2041 gtcggtcatg gccgccctgc acccggcaga tcggcgacca tcgcgacgtc cttgtcgccg
     2101 cggccgctca ggttgatgag cacgaggtcc ccggactgcc agcggccctt cgcccgcgac
     2161 acccaagcca cggcgtgcgc gctttcgagg gccggcacga tcccctcgag ccgggccagc
     2221 gaggtaaacg cgtccagcgc ttcacgatcc gtcacggtct gatacaatac gcgcccctgg
     2281 tccttgagaa aagcgtgctc cggtccgacg ccggggtaat ccagccccgc cgagatggaa
     2341 tgtgccggac ggacctgacc gtcggcgtcc tgcaacaggt agctccggct gccgtgaaac
     2401 acgccgggcc gccccaccga cagcgacgcc gcgtgccgtc cggtctcgat cccctccccc
     2461 gcggcttcca cgcccaccag ctcgaccgcc gcgtcgtcga ggaaagcagc gaagatgccg
     2521 atcgcgttgg atccgccgcc cacgcaggcg accacggtgc gcggcaactt cccgaagcgg
     2581 tcgaggcatt ggtcccgcgc ctcgcggccg atcacggact ggaagtcccg caccattcgg
     2641 gggaacggat cgggtccgac gaccgagccg atgatgtagt gggtatcggt gacgtgcgcg
     2701 acccagtcgc gcatcgcctc gttggtcgcg tccttgagcg tgcgcgttcc cgatgtcacc
     2761 gccaccacgc gtgcgcccag cagctccatc cggtagacgt tgagccgctg ccgccgcatg
     2821 tcctcttcgc ccatgtacac cacgcactcg aggccgaacc gcgcgcacac cgtcgcagtc
     2881 gcgaccccgt gctggcccgc gcccgtctcg gcgatgatgc gccgcttgcc catgcggcgc
     2941 gccagcagcg cctgccccag cgtgttgttg atcttgtgcg cgccggtgtg gttcaggtcc
     3001 tcgcgcttca ggacgacggt gaccccgggc cccagcccgg ccgcgaagcg taccgcctcc
     3061 gtgagcggcg tgggccgacc cacgtagtcg cgctgcagcc ggtcgagctc cgcccagaac
     3121 ccgggatcga cgcgggcggc atcgtacacc gccgcgagct cgtccaacgc ccccatgagc
     3181 gtctcgggga cgtagcgtcc gccgtaggga ccgaacgccg cttcccgcgt cgccgtcatg
     3241 ccgccattcc acgcggctgg cgcggcacgc cgcacagcgc gtgcacggcc gtgcgggggt
     3301 cggcgtgacg caccagcgcg gtccccacca gcaccagatc ggcgcccgcc gccgccagcc
     3361 gctccacgtc ggcccgcgat tcgatgccgc tctccgcgac ggcgacgagg tggcgcggca
     3421 cggcgcgcag cactcgctct ccgaccgcca ggtcgacctc gaagctgtcc agatcacggt
     3481 tgttcacgcc gacgacggcg gcgccggcgg cgatggcgcc gtcgagtttg tccacgctgt
     3541 gcacttcgac cagcacgccc agctccagtc ggctcgcggc agcgatgagc tcgcgcaacc
     3601 ggatcgcggt cagaatccgc gcgatgagca gcaccgcgga cgcccccgca gcccgcgccg
     3661 ccagcagctg cacctcgtcc aggatgaaat ccttgcacag cacggggacc ggaacggcgc
     3721 cggccacacg ctgcaggtct tccagcgacc cgccgaagtg ctccggctcg gtgagcaccg
     3781 agatggccac cgccccgccc gcggcatagg cgcgcgcgtg ggcgaccgga tcgaggtccg
     3841 cgtgaatcgc cccggccgac ggggaacgcc gtttcacctc ggcgatcacg cccaccgagc
     3901 cgtccggggc gagcccgccg aagaagcgcg gcggggagac gttcgcggcg cggtgctcca
     3961 gctcgccccg ccggggctcc agccgcgcca cgcgctccct cgtatccgct aggattcgct
     4021 ccagctggtg gctcatcgcg cttgcattcg ggtggtattc gggtacatta ttcttcggtc
     4081 aattttcggg agattcggat gccgctcacc cgtcgccagc gcgagatcct cgactacctc
     4141 accgagtaca tcgggcaacg cggttacgct cccagcttcg aggaaatcgc cgagcggttc
     4201 cgcttccagt ccctggccac cgtgcacgag cacctcacca atctcgagcg caaaggctac
     4261 atccgccgca cgccgaacga gagccgggcg atcgagatcg tgccgccacg cggtcagacc
     4321 ggcgccaccg agctcccgct gctcgggctg gtcgcggccg gccagccgat cgagacgatg
     4381 tcggacgcga acgacacgat cgcggtgccc gacacgctgc tgccgcgccg cggccgcagc
     4441 tacgtgctca aggtgcgcgg ccgctcgatg atcgacgagc agatcaagga cggcgactac
     4501 atcgtcgtgc acgagcgcgc ccaggccgag aacggcgaga ccgtcgtggc gctcgtcggg
     4561 ggagacagtg ccacagtcaa gaaatactat cgcgagcccg gcggctggat ccggctgcag
     4621 ccggccaacg ccgagatgac cccgatccgg gtcaacgagc gcgacgtgat cgtacagggc
     4681 gtcgtcctcg gcgtcatccg tcggtactag gactggcgcg gcgcagccgc tccagcgccg
     4741 cgcgcgccgc gccggagtcg agcgccgcgc gtgcccgttc gacgccgtcc gcgtaactcg
     4801 tcgtgaggcc cgctgcgtag atcgccgcgg ccccgttgag cagcaccgcg gcccggccgg
     4861 ccgcatcctc atcgtcctcg agcagccgct ccagccgggc ggcgttggcc gccggctccg
     4921 ctccgcgcag cgcgtcgaca tcgtgccccg cgagcccgta gtccgacggg tcgagcgtcc
     4981 agtgcgagac atcaccgtcg cgcacctccc agatcgaggt ctcccccacc ggggagattt
     5041 cgtccatccc gacacggccg tgcaccacca gcgcatgctc gacgcccagc cggcacaggg
     5101 cgttggccac gagcggcccg agcggcgcat cggcaacgcc gatcacctgg cgccgcacgc
     5161 ccgccgggtt ggcgagcggc ccgagcaggt tcatcagcgt cgtgaccccg agctccctgc
     5221 gcaccggcgc gacgtgacgc atggcggggt gaaagttggg ggcgaacagg aacgcgacgc
     5281 ccgcctggcg caacacgcgc actgccccgc tcgcatcgag acccaccgag acgcccagcg
     5341 cctcgaagac gtccgcggag ccgcagcgcg acgtgtagga gcgattgccg tgcttggcca
     5401 cggtcgcgcc ggaccccgcg gcaaccagcg ccgcagcggt cgagatgttg aaggtggtga
     5461 ccgcaccgcc gcccgtgccg gcggtgtcca cgagatgggg ggcgtcgatc tcgacccgca
     5521 ccatcgcctc gcgcagcgcc cgggcggcgc ccgcgacctc gtcggcggtc tcccccttca
     5581 cgcgcagtcc catcagcagg ccgcccacct gggcgggcgt cgcctcgccg cgcatcacgg
     5641 cgccgaagac ttcggccgcg agtgactccc ccagcgattc gcgggcggcc agagccagaa
     5701 tggcccgttc gaggggaccg gtgaggcgtt gggtcatgcg cgggcggggg gtgcggaaag
     5761 ctaccccgaa aaatcggctt ttgtcaaatg ggacaacggg ttaagcgtct tgaattgacc
     5821 gtcccccttt ctctatcttt ggctggtgac gagccggccc ttcctcgccg tggtgcagta
     5881 cgacggggga cagttcgtgg gctggcagcg ccaaccggcc ggacggacgg tgcaggcgga
     5941 attcgagacc gtgctggcgc gcctgatggg cgggggcgag cccgtgacgg cgatcggcgc
     6001 gggccgcacc gacagcggag tgcacgccgt cggccagggc gtcggattcc gcggggccgt
     6061 gcgctggagc gcggatgtgg acggtttgcg gcgggcgttg aacgcgctgt tgccgcgcga
     6121 catctgggtc gagcgggtgc atgcgatgcg ggacgacttc catgcccgcc ggagcgccac
     6181 cgcacgccgc taccgctacg agatcgggac cgacgacgcc agtcactcgc cgtttcgccg
     6241 gccgttcgaa tgggccttgg ggcagccgct cgagcaggag ttgctcgcgg cagcggcccg
     6301 tacgctcacc gggacgcatg atttccgcgg cctctcggcc gccggcccgg ccaggcccga
     6361 ctaccgatgc cgcgtggcgt tcgccggctg ggcgccgcga gcggacggga cgggcgtgac
     6421 gttcacgatc gagtcggacc ggtttctgca ccgcatggtg cgcttcctcg tgggcacgat
     6481 ggtcgacatc gcgctcgcgc gacgcctgcc gtcggacctt tcccagctcc tggccgcgac
     6541 cgacaaccag gccgccagcc cgccggctcc gccgcagggc ctctacctgg atgcggtgcg
     6601 ctatcctccc gacctctacg ccgaggcgca cgcatgaagt tcttcctcga cacggcttcc
     6661 ctgaaggaca tcagctgggc gacgcaggcc ggcctgatcg acggcgtcac caccaatccg
     6721 tcgctcctcg ccaagcaggc cggcgacctc gacccgcgcg acgtactgaa ggagatctgc
     6781 tcgctggtcg aggggccggt gtcggccgag gtggtcgcgg tagaagcgga cgccatggtt
     6841 cgcgagggcc agtcgctggc gcggatcgcg gacaacatcg tggtgaagat cccgatgctc
     6901 gaggccggca tggtggcggt gcggcgcctc gccgccgcag gcatccgcac caacgtcacg
     6961 ctctgcttct cgagtgtgca gtgcctgatc gcggccaagg cgggcgctac ctacgtgagc
     7021 ccgttcatcg gacggctcga cgacgtgggc caggacggca tggacgtgat ccgcgaagcg
     7081 cggcagatct tcgacaacta caccctggcg acgcagatcc tcgccgcttc gatgcggcac
     7141 ccgcgccatg tcgtggaggc ggcgatgatc ggcgccgacg tcgccacgct tccccccgac
     7201 gtgctgcgca agctgctgca gcacccgttg accgatcgcg ggctggagca gttcctggcc
     7261 gactggcagt cccttgccgc ccgggccaag gcgtcggtgt gacctgggaa tcgccgctct
     7321 cctcgcgcta cgcgtccccg gcgatgcagg cgctgtggag cgagcgccgc cgcatcggtc
     7381 tgtggcggcg gttgtggctc gcgctgatgg agagcgagcg cgagctgggg ctcgaggtcc
     7441 cggccgaggc gatcgcgcaa ctgcgcggcc acctggacga cgtggatctg ccccgggcgg
     7501 cggaatacga gaagcgcatg cgccacgacg tcatggcgca catccatgcg ctgggcgagc
     7561 aggcgccggc ggcgcggccc tacctgcacc tcggcgccac cagcgcgttc gtcaccgaca
     7621 acgccgatct catcctgatg cgcgagggcc tcggcctgct gctggggcgg gtcgcggcgg
     7681 tcctcgtcgc tttgagccac acggcgcgtc gccatcgtgc tctgccctgt ctcgcctaca
     7741 cgcacttcca gcccgcccag ctcacgaccg tgggcaagcg cgccaccctg tggatgcagg
     7801 aattcctgct cgatgccgag gagctgctgc accgcggcga ggcgctgcag ttccgcggcg
     7861 tgaagggaac gaccggcacg caggcgagct tcctcgagct gttcgacggc gaccacgaca
     7921 aggtgctcga gctggacgtt cgcgtggcgg cgaagctcgg attctcccgc gtgttcccgg
     7981 tcacggggca aacctacacc cgcaagcagg acgcccaggt gctggccgcg ctcgccggga
     8041 tcgccgcgtc ggccgccaag ttcgccaccg acctccgcct gctgcaacac gagggggagc
     8101 tgctcgagcc gttcgagagc gagcaggtcg gctcctcggc catgccctac aaacgcaatc
     8161 cgatgcgggc cgagcggatg acgagcctgg cgcgtttcgt catcgagctc gaaggcaacg
     8221 cgtggcacac cgccgcggag caatggctcg agcgcacgct cgacgacagc gccaaccgcc
     8281 gcctcgtgat ccccgaagcc ttcctcgcga ccgacgcgat tctcgtgctg gcgaccaacg
     8341 tcgccgccgg gctcgaggtg cgggaggcgg tgatccgccg ccacgtcgag gacgcgctcc
     8401 cctacctcgc caccgagcgt ctgctgatgc gcgcggtgaa ggcgggagga gaccgtcagc
     8461 gcctgcacga ggtgatccgc gtgcacagcc tggcggtgtc gcagggggtg gccgagcgcg
     8521 gcgcgacgaa cgacctgctc gcccgcctgg ggtccgaccc ggcgttccgc gcactgggca
     8581 tcgccgcaca tccccacgag ctcgacccgg cccagtatgt gggccgcgcg cccgagcagg
     8641 tcgatcactt cctggacgag gtactgcccg ccgtgctgca tcgcatcgac gccgtggttc
     8701 ccgccgccgg caccgccgag gtgcaggtat gacgaccgtc acgcccctgg tggcgagcca
     8761 cctccccctg ccgctgttgc gccggggcaa ggtacgcgag atgtacgagg tggacggcga
     8821 cacgctgctc atggtcgcta gcgaccgggt gagcgccttc gacgtcgtgc tgcgcgagcc
     8881 cgtggccttc aagggcgctg tgctcaccca gctgtcggcg ttctggttcc gccgcctcgc
     8941 gcacgtgctg cccaaccacc tggtcacggc ggacgcgggc gagatcgtcg cgcgcgtgcg
     9001 gaccctggcc ccgcaccgcg ggacgctgca gggccgcgcg atgctggtgc gccgcacgac
     9061 gcccgtcgcc ttcgaatgcg tcgtgcgcgg gtacctcgcc gggtcggcgt gggccgagta
     9121 caagcgatcg ggcacgctcg cgggtgaggc cctgccctcg gggctcgtgg aaagcgcccg
     9181 gctggagccg gccatcttct cgcccgcgac caaggcggag accggccacg acgagaacgt
     9241 cacgttcgcc catatggcgc aggcactggg cggaccgctc gcggacgcgc tcaagcgcgc
     9301 gagcctcgcg ctctacgagg agggtcgcgc ctacgccgcc ggccggggca tcatcatcgc
     9361 cgacacgaag ttcgaattcg gcacgaccgc ggacggcacg ctgctgctga tcgacgaggt
     9421 gctgaccccg gattcctccc ggttctggcc ggccgatcgc tatcagccgg gaaccggcca
     9481 gccgagcttc gacaagcagc cgttgcgtga cttcctctcc ggtctcaagg agcagggaaa
     9541 gtgggacggc aacgatgctc cgccggcgct tccgccggtg gtgatcgcgg ccacgagcgc
     9601 ccgctaccgc gaggcgtatc gcctgatcac cggcgcctcg ctcgaggtca cggcgtgaaa
     9661 atggctccgg agggatggcc cttcatcgcc ctcggatggg gtgtggccgc cctcggggcc
     9721 tgggcgacgc tgcgctgggg cggctggtgg tgggtgatcg aatcggtcgt gctgctgctc
     9781 gccgtctggc tgctcgtgtt cttccgcaac cccgagcgta ccggcccgcg cggggaccgc
     9841 tacgtgatcg caccggccga cggcaagatc gtcagcacgg tggagaccga cgagccggcg
     9901 tacctccgga cccgggcgct gcgaatctcg atattcatga gcgtgttcga cgtgcacgtc
     9961 aaccgctacc ccgtctcggg caccgttgcg taccgccact acaacgccgg gcagttcctg
    10021 cacgccgctg ccgacaaggc cagcctggac aatgaacagg ccgccatcgg gatcgaggga
    10081 ccgcacgggc cggtgttggt ccgtcagatc gcggggctca tcgcccgccg tatcgtcacc
    10141 gacgctacgg tcggcgaccg ggcagcccag ggcgcgcgac tcggcatgat ccggttcggc
    10201 tcccgcgtgg atgtctatct ccccctcacc gcccggccgg cgtcccgcgt gcaggtcggc
    10261 gatcgcgtcg ccgccggcgc gacggtgctg gcggagtacg gcccatgacc gccacccctc
    10321 ccaaggcacg gcgtggtgtg cgacgcgtgg tcgtcatcgt cccgagcctg ttcacgctgt
    10381 tcaatctgtt cttcgggatc tggtcgatca cgctcgccgc caaaggcgag ttctatcgcg
    10441 ccagctggtt cctcgtcttc gccgggattc tcgacaccct cgacgggcgt gtcgcccggc
    10501 tgtccaacac cgggacccgt ttcggcgccg agctcgacag cctggtggac gtggtgagct
    10561 tcggcgtggc ccccgcctat ctcatgtacg agctccattt cgccacggcc ggtccggcgg
    10621 cgtggatctt ctgctacttc tacatcatgg gcgcggccat ccgcctggca cggttcaata
    10681 tcacccaggc gggccgggcc aaggcgcact tcgtcgggtt gccgtcgccc gcggccggcc
    10741 tcacgctcgc cacctattac ccgttcacgc agaccgatct gtacgcgcag atgcggaccc
    10801 tcccctggcc gctgctcctg aacttcctca tgatcgcgtt gacgatcctc atggtgagca
    10861 acgtgcggta cgccaccttg ccgcgcgcca gcctgcgctc ctggcgtggg ctcttgggac
    10921 tcctgacgct gctgtgtatc gtcttcttcg gcatctggca gcacgacgcg ttcttcttcc
    10981 cgctggggat cgtgtacatg gtctatggcg tggcccgggc cgccctcctg gggttcttcg
    11041 agaacggcga cgattcgggg gagaccgata tcgccggtcc gatcgtgatc accgacgcct
    11101 cggccgatcg cgtggacgac gcgttcggcc cgcgctcccg gggcatttcc ccccggggac
    11161 gccactgatg cccgccttcc gggtagagat ccgggtgatg ccgcgcgccg cgctgctcga
    11221 cccccaggga caggcggtga gtcacgcgct ccacgccctg gggttcgccg acgtggggga
    11281 cgtgcgggtg ggcaagcacc tcgtcgtccg gctggaggcg tcgacgagcg atgaggccgc
    11341 ggcgcgggcg cgggcgatgt gcgatcggct cctcgccaat cccgtgaccg aggactacca
    11401 gctgctggtg gaggccgcct gatgcatcga gtcgccgtgg tggtgttccc ggggtccaat
    11461 tgcgagaccg agaccgtcga cgcgtcgcgg gccgcgggct cggacgccta cgaggtgtgg
    11521 catcgcgaga ccgatctgcg gcaggcggac gcggtcatcc tgccgggcgg gttcagctac
    11581 ggcgactacc tccgttccgg cgccatcgcc cgcttctccc cggtgatggc ggcggtcgcc
    11641 gcgcacgccc gcgccggtgg cgccgtgctc ggcatctgca acgggttcca gatcctgtgc
    11701 gaagccggcc tgcttccggg cgggctgatg cgcaacgtcg ggttgcggtt cctgtcgcgc
    11761 cccgtgcggg tgcgtgtcga agcgaacgac acgccgtgga cgcggggctt cgttcccggc
    11821 gaggagatcg tggccccggt cgcgtccggc gacgggcgct atgtggcgga tgagcgcacc
    11881 gcggccgagc tggaagccga gggacgtatc gtctggcggt acgttggaga gaaccccaac
    11941 ggctcgaccc gcgccatcgc cggcgtctcc aacgccagct gcaacgtggt tggtatcatg
    12001 ccgcaccccg agcggcgcaa tgcgtccgtt ctcggcggca ccgatggcgc ccgtgtcttt
    12061 ctttccctgg aggctgatca tgcgacgcag catgctggtg gtgtgcgcgc tgttggcggg
    12121 caccacgctg ggctgtgaca ggatcaagca ggctctcgac aagcgtcgga accgtggccg
    12181 cacgccagcc acccagaccg cgataagcga taccaccaag aagccggcgc ccgcgccggg
    12241 cacgcccacg acgccggcgg gcagcaccca aacgacccct gggaccccgg caccgggtgc
    12301 cgcgacgccg cccgcatcgc ctcgccccac gccgcccagc accccggcgc cccgcgcgcc
    12361 gcagacggtg ttccgcgacg agccgtacaa ctccgccgat accggcacca tcgctcccgg
    12421 catgactcag gcggatgtgg cgtcgctgtg ggggcgggcc tcggccgtac ggcacgcggg
    12481 ggagtacacc tatctgcact accccaacgg ctgcgagctc acctgcggca ccgacgacgt
//
LOCUS       DBNGZW010000706         2560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1072043, whole genome shotgun sequence.
ACCESSION   DBNGZW010000706 DBNGZW010000000
VERSION     DBNGZW010000706.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1072043"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="AC16JC_16660"
     CDS             complement(<1..483)
                     /locus_tag="AC16JC_16660"
                     /inference="COORDINATES: protein motif:HMM:NF014385.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HIR5166384.1"
                     /translation="MRYVVLVLAGTAAGIVPGEARGQEVPRISPPALVLTRREAVDTA
                     LARNPQLRVAREQVAQARARAVQTGAFPDPTLTGDLTGQTGATRFRSNTGSDVGIGLL
                     LPFPTKFLLRRQVGGADVAAAQFDYEQLRQETASLAAQSYDGLIVLLRHRADLIDGDS
                     L"
     gene            complement(616..975)
                     /locus_tag="AC16JC_16665"
     CDS             complement(616..975)
                     /locus_tag="AC16JC_16665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166385.1"
                     /translation="MQHWSTGVHRGVARGIATLLFVLQAGAGGAVALAHAADSLGGPA
                     TLESHHSAQCVTVHDAARCAQCQYQATRVLPAARRRVPLASGATHVQKTCELARLAPP
                     QPPLRATPPRAPPQSVA"
     gene            complement(984..1934)
                     /locus_tag="AC16JC_16670"
     CDS             complement(984..1934)
                     /locus_tag="AC16JC_16670"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIR5166386.1"
                     /translation="MGRAQRGKRPFRAADLATRVSSGVTLIFHDDLLGAHVSSLGGVA
                     HAPERGRTIGASAIQVFTKTPNQWREPAIEAAHAEAFGKALTRSRIRAVVAHDSYLIN
                     LASPDAVLRARSEESFTRELERCRMLGIPWVVSHPGNFMDDRASGLERNAQSYAACLT
                     RVEGPGVLIEGTAGAGTALGATFEELAALREALPPAVRPRVGFCLDTAHLHAGGYDVA
                     ADIAAVWREFDRVVGLAHLKCLHLNDSLVARGSRIDRHAWIGEGAIGPEAFRRIMQDP
                     NLVGVIKIIETPKADDPVRHDRRMLRRLRAYARGTPSTAR"
     gene            complement(1856..2518)
                     /locus_tag="AC16JC_16675"
     CDS             complement(1856..2518)
                     /locus_tag="AC16JC_16675"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HIR5166387.1"
                     /translation="MPLAPHTRSARRRMVRRQLAARGVRDARVLATMARIPREWFLAD
                     GLDRQAYRDGPLPIGNGQTISQPYIVAVMTAALGAHRRHRVLEIGTGSGYQTAVLGRL
                     VGHVYTVERLPDLLVEAEERLRRLGIGNVTTRLGDGAAGWPEAAPFDSILVTAAIPHV
                     PPPLLEQVTPGGRIVAPVGDQAEQQLVILERRADGWAERSAGSVRFVPLISRLAFPAE
                     SP"
BASE COUNT          375 a          920 c          898 g          367 t
ORIGIN      
        1 cagggaatct ccgtcgatca ggtcggcacg gtggcgcagg agcacgatca gcccgtcgta
       61 actctgcgcc gccagcgagg ccgtctcctg gcgcagctgt tcgtagtcga actgggccgc
      121 cgccacgtcg gcgccaccca cctgccggcg caacaggaac ttggtcggga acgggagcag
      181 cagtccgatg ccgacgtcgc tccccgtgtt cgagcggaac cgcgttgcgc ccgtctgtcc
      241 ggtcagatcc ccggtgagcg tcggatcggg gaatgcaccg gtttggacgg cgcgagcccg
      301 tgcctgcgcc acctgctccc gcgcgacgcg cagctgcggg ttgcgggcca gcgcggtatc
      361 gacggcctct cggcgcgtga gcaccagagc cgggggtgag atgcgcggga cttcctggcc
      421 ccgcgcttcc cccggaacga tcccggcggc tgtccctgcc agtaccagaa caacatatcg
      481 cacgtgcgac atctcccaat gcctccgaac ggagtggtcc agcaggggag cgcggccgtc
      541 caacgacggt gttcgcgagt acccgtggac gctacgcgcg catcgtacct ctcgacgcgc
      601 ggtgcttgac cgagctcagg cgacagactg gggaggtgcg cgaggtgggg tggcgcgcag
      661 gggcggctgt ggcggtgcga ggcgcgcgag ctcgcaggtc ttctggacgt gggtggcgcc
      721 cgacgcgagc ggcacacggc gccgcgccgc cggcagcacg cgggtcgcct gatactggca
      781 ctgcgcgcac cgcgcggcat cgtgaaccgt cacgcactgc gcgctgtgat gcgactcgag
      841 cgtggccggc ccgccgaggg aatcggcggc atgcgccagc gccacggcgc cgcccgcacc
      901 cgcttgcaga acgaacagca gggtcgcgat gccgcgtgcc actccgcgat gcactccggt
      961 cgaccagtgt tgcatgatgg ccctcaacgc gccgtggacg gggtgccgcg cgcgtacgcg
     1021 cgcagccgcc gcaacatgcg ccggtcgtga cgcaccggat cgtccgcttt cggcgtttcg
     1081 ataatcttaa tcactcccac gagattcggg tcctgcatga tgcgccggaa cgcctcgggg
     1141 ccgatcgcgc cctcgccgat ccaggcgtgc cggtcgatgc gcgatccccg cgccaccagc
     1201 gaatcgttga ggtgcaggca cttgaggtgc gccagtccca ccacccggtc gaactcccgc
     1261 cacaccgccg cgatatcggc ggccacatca tacccgcctg catgcagatg ggcggtgtcg
     1321 aggcagaaac ccacgcgcgg ccgtactgcg ggcggcagcg cctcgcgcaa cgccgccagc
     1381 tcctcgaacg tcgcccccaa cgccgtcccg gccccggcgg tcccctcgat gagcaccccc
     1441 ggtccttcca cgcgggtcag gcaggcggcg tagctctgcg cgttgcgttc gagtccggag
     1501 gcgcgatcgt ccatgaagtt gcccgggtga ctcaccaccc aggggatgcc aagcatccgg
     1561 cagcgttcca gctcgcgagt gaagctctct tcactgcgcg cccgcagcac cgcatccggc
     1621 gaggccaggt tgatcaggta gctgtcgtgc gccaccaccg cgcggatcct cgaccgggtc
     1681 agcgccttcc cgaaagcctc ggcgtgggcc gcctcaatcg ccggctcgcg ccactgattg
     1741 ggtgttttgg tgaacacctg gatggcgctc gcgccgatcg ttcgcccgcg ttccggggcg
     1801 tgggccacgc cgcccaggct cgacacgtgc gcgcccagca ggtcgtcgtg gaagatcagg
     1861 gtgactccgc tggaaacgcg agtcgcgaga tcagcggcac gaaacggacg cttcccgcgc
     1921 tgcgctcggc ccacccgtct gcccgccgct ccagaatgac cagctgctgt tccgcctggt
     1981 caccgaccgg cgccacgatg cgcccgcccg gcgtcacctg ctcgagcagc ggtggaggca
     2041 cgtgtgggat cgcggcggtg acgaggatgc tgtcgaacgg cgccgcttcg ggccaccccg
     2101 cggctccatc gccgagtcgc gtggtcacgt tgccgattcc gagtcgacgc agccgctcct
     2161 ccgcctcgac cagcaggtcc ggcagccgct cgaccgtgta gacgtgcccc accagccggc
     2221 cgagcaccgc cgtctggtag cccgatcccg tgccgatctc gagcacgcga tgccggcggt
     2281 gcgcgccgag cgcggcggtc atcaccgcga cgatgtaggg ctgcgagatc gtctgtccgt
     2341 ttccgatggg cagcggaccg tcgcgatagg cctgccggtc gaggccgtcg gcgagaaacc
     2401 actcgcgcgg aatgcgggcc atcgtcgcca gcacgcgggc gtcgcgcaca ccgcgcgccg
     2461 cgagttgccg gcggaccatg cggcggcggg cggagcgggt gtgcggggca aggggcatcg
     2521 cccgaaagat gcgtgtgggg accggggaac ggggagtggg
//
LOCUS       DBNGZW010000707         6178 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_204049, whole genome shotgun sequence.
ACCESSION   DBNGZW010000707 DBNGZW010000000
VERSION     DBNGZW010000707.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6178)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6178)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6178
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_204049"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="AC16JC_16680"
     CDS             complement(<1..256)
                     /locus_tag="AC16JC_16680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5166388.1"
                     /translation="MSEIALYRRLFRQARPYWLYFSGLLVLGLLAAPIALLAPVPLKV
                     VVDSVLGTRPPPPWLQAILPGSATATAAVTLLVAAAMLVAV"
     gene            complement(249..2831)
                     /locus_tag="AC16JC_16685"
     CDS             complement(249..2831)
                     /locus_tag="AC16JC_16685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015152963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5166389.1"
                     /translation="MARAYSVDSVETMAHGLVATTGERVLPRGEARPTEVARPRARGK
                     FLFAGEEKLYVRGVTYGAFRPDARGNEYHDLTIIERDFAQMAANGINAVRIPHTTPPR
                     GLLDAALRHGLRVMVGLSAEQYFGYLIDRAPGAPDIESLIRARVRSCAGHPALLCYAL
                     GNEIPASIARWLGPRKIERYLERLCRVVKEEDPDSLVTYVNYPTTEYLRLPFLDLLCF
                     NVYLESQDRFEAYLSRLQSLAGGRPLVMTEVGLDSLRNSEATQARSLDWQVRTAFAAG
                     CAGLFVFSWTDEWHRHGSEVEDWAFGLTHADRSPKPALEAVREAFAAVPFAPTQSWPR
                     ISVVVCTYNGARTIRDCCEGLARLVYPNYEVIVVDDGSTDDSAAIAGRYDVRLIRTPN
                     RGLSNARNTGLEAATGEIVAYIDDDAYPDPHWLTYLAATFLGTTDAGVGGPNIAPAGD
                     GAIAECVSRSPGGPVHVLLTDREAEHIPGCNMAFRKSCLQAIGGFDPRFRTAGDDVDI
                     CWRLRARGWTLGFHAGAMVWHHRRNSLRAYWKQQIGYGGAEGQLAKKWPEKYNGAWHL
                     RWAGRMYGSGLSYILGLRRARVHHGVWGLAPFQSLYEPSPTLLGALPQMPEWYLMIAL
                     LAGMAALGDAWGPLKVTRLLLLAALLPPLAQAVLGGATASFPGLQRAWPTWFRRRLLT
                     TFLHLLQPVARLHGRLREGLTPWRGRCPMRPGGLRRAEASLWDERWQELEGRLSALET
                     RLRAAGAHVVSGGPQERWDLELRPGCFGAARLIMAVEEHPASHQLVRVRWWPILLLPG
                     AALLAAFGALAVGAALDNAWVAAAILGLAALLAGGRVVLECGAATAMVGETVGGRRAE
                     HCDE"
     gene            3375..4634
                     /locus_tag="AC16JC_16690"
     CDS             3375..4634
                     /locus_tag="AC16JC_16690"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR5166390.1"
                     /translation="MTTYRSTQVRTLLLYAQDTRGLGHITRTLAITRHVLDAYPDVVA
                     YIATRSHFAHSYTLPERCDYIKLPSRQTPWHIERPPEDDQESKEHFRRLRGIVLRDVA
                     LGLAPDLVLVDHEPLGTNAEFKAGLWALKEESPHTRFVFGLRDIMDDPERIRAEWREM
                     GVYEALEGLFDGITVYGSPSLFDPVKAYGIPASVRHKLHYCGYVVRDPPEVDCAAVRR
                     QYGLPEDGQLVLATVGSGYDGYPVLDVALSALERVQATRPGLHAILVTGPFMPPQLQA
                     ALQARANGFCRVVTTADTFHLMAAADAVVSMGGYNSVCEGLSVGRPLVIVPRATHKVE
                     QKMRAELLADQGLAKCVLPQALTPESVEGALDWALAVDPELHTRRVRAVLPAFDGAAR
                     LTGYLSHWLGKSRFEDHSLAHALEAHG"
     gene            4747..5250
                     /locus_tag="AC16JC_16695"
     CDS             4747..5250
                     /locus_tag="AC16JC_16695"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR5166391.1"
                     /translation="MSSKVIRYAGDADRFRLEEWTTAWGDGAATKDCVWVRGGYALVV
                     AVANDRVVCIRQYKKAAQQVLLALPWGGIEPGESPLDAGRRELEEETGFSFGAAEVHG
                     PFYDLPDKSTGGHWVIVARRAFRKAAAAPDAGELIFGTELIPLRRLWQHRFPVLMHVG
                     ALRLARL"
     gene            5265..>6178
                     /locus_tag="AC16JC_16700"
     CDS             5265..>6178
                     /locus_tag="AC16JC_16700"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HIR5166392.1"
                     /translation="MRARFKPTPEQRSALREQLTRVQQPDTALRILRPLLPEDFAPAH
                     VAVTLQSAHPDRFVVRAECRSVDGGTSVYALKAYCDDFVHQVWNYSRALALVHRPNHR
                     GLCLPIRCIPEERILISPWVQGEFLSDVVDERRLQLLRQAALLAADVHRLPLVPEELT
                     SAEMFVADTLARCERLRSKWPEAGSLVLPLMDALVRASACLEPAEPGPVHGDMSPGQF
                     LWTGERLALLDIDMFGYADPAYDVGHFQAQVERRCIVDLTARPLANTWLACFEETYRS
                     AMPRVSPRNIAFYRGLTLVRKMYTVCRR"
BASE COUNT          962 a         2182 c         2118 g          916 t
ORIGIN      
        1 tgactgccac gagcatggcg gcggccacca gcaacgtaac cgccgcggtc gccgtcgcgg
       61 accccggcag aatcgcctgg agccagggcg gcgggggccg cgtcccgagc acggaatcca
      121 cgacgacctt gagcggaacc ggggcgagca gggcgatcgg agccgcgagc aacccgagga
      181 cgaggaggcc ggagaaatag agccagtagg gacgcgcctg ccgaaacagc cgccggtaca
      241 gggcgatctc actcatcgca gtgttccgcg cggcggccgc cgacggtctc accaaccatg
      301 gcggtcgccg caccgcactc gaggaccact cgccctcccg ccagcagggc ggcgaggccg
      361 aggatcgcgg cggccaccca cgcattatcc agggccgcac ccacggcgag cgcgccgaag
      421 gcggccagca acgcagcgcc gggcaggagc aggatgggcc accagcgcac gcgcacgagc
      481 tgatgcgaag ccggatgctc ctccaccgcc atgatcagcc gggccgcccc gaagcagccg
      541 ggccggagct cgaggtccca gcgctcctgc ggcccgccgg acaccacgtg ggcgccggcg
      601 gctcgcagcc gagtctcgag cgcggacagt cggccttcca gctcctgcca gcgctcatcc
      661 cacagggagg cctcggcgcg ccgcagtccc cccggccgca tgggacaacg gccgcgccat
      721 ggtgtgaggc cttcccggag gcgtccgtgc aggcgcgcga ccggctgcag cagatggagg
      781 aacgtggtga gcagccgccg ccgaaaccag gtcggccagg cacgctggag cccgggaaag
      841 gatgccgtcg ccccgcccag gaccgcctgg gccagcggcg gcaggagcgc ggccaggagc
      901 aacagtctgg tgaccttcag cggaccccac gcgtccccga gcgcggccat gccggccaga
      961 agggcgatca tgagatacca ctcgggcatc tgcggcaggg cgcccagtag ggtgggggac
     1021 ggctcgtaga gggactggaa gggagccagt ccccacaccc cgtggtggac gcgagcgcgg
     1081 cgcagcccga ggatgtacga gaggccactg ccgtacatgc gaccggccca ccgcagatgc
     1141 cacgcaccgt tgtatttctc gggccatttc ttcgccagct gaccctccgc accgccatag
     1201 ccgatctgct gcttccaata ggcgcgcagc gagttgcgcc gatgatgcca gaccatggca
     1261 cccgcgtgga agccgagcgt ccagccccgc gcccgcaatc gccagcagat gtccacgtcg
     1321 tcccccgcgg tgcggaaccg cgggtcgaac ccgccgatcg cctgcaggca ggacttgcgg
     1381 aacgccatgt tgcaccccgg aatgtgctcg gcctcgcggt cggtgaggag gacgtggacg
     1441 ggtccgcccg gggagcggct cacgcactcg gcgatcgccc cgtccccggc cggcgcgatg
     1501 ttcggaccgc ccacgcccgc gtccgtggtg cccagaaatg tggcggcgag gtaggtgagc
     1561 cagtgaggat cgggatacgc gtcgtcgtcg atgtaggcga cgatttcccc cgtggccgcc
     1621 tccaggccgg tgttccgcgc gttggagagg ccgcggttcg gcgtccggat caaccgtacg
     1681 tcgtaacggc ccgcgatggc ggcgctgtcg tcggtcgacc cgtcgtcgac cacgatgacc
     1741 tcgtagttgg gatataccag ccgcgcgagc ccttcgcagc aatcccggat cgtgcgcgcc
     1801 ccgttgtagg tgcacacgac gaccgagatc cgcggccagg attgcgtggg cgcgaacggc
     1861 acggccgcga acgcctcgcg gaccgcttcg agcgcgggct tgggcgagcg gtcggcgtgc
     1921 gtgaggccga aggcccagtc ctctacctcc gacccgtggc gatgccactc gtcggtccaa
     1981 gagaagacga agaggccggc gcacccggcg gcgaacgcgg tccgcacctg ccagtcgagc
     2041 gagcgcgcct gggtcgcctc gctgttccgc aggctgtcga gcccgacttc ggtcatgacg
     2101 agcggccgcc ctccggcgag gctctgcaga cgcgacaggt aggcctcgaa gcggtcctgc
     2161 gactcgaggt agacgttgaa gcacaggaga tcgagaaacg gcaaccggag gtattcggtg
     2221 gtcggatagt tgacgtaggt gaccagactg tcgggatctt cttccttgac cacccggcag
     2281 aggcgctcga ggtaccgctc gatcttcctg gggcccagcc agcgggcgat cgaggcggga
     2341 atctcgttgc ccagcgcgta gcacagcagc gccgggtggc ccgcgcagct gcggacccgg
     2401 gcgcgaatca acgactcgat gtccggcgcg ccgggagcgc ggtcgatgag gtagccgaag
     2461 tactgttcgg cgctcagccc caccatgaca cggaggccgt gccgcaacgc cgcatccagc
     2521 aggccgcggg gaggcgtcgt gtgcgggatg cgcacggcgt tgatgccgtt cgcggccatc
     2581 tgcgcgaagt cgcgctcgat gatagtgagg tcgtgatact cgttcccccg cgcgtccggg
     2641 cgaaaggcac cataggtcac gccgcggacg tacagcttct cctctcccgc gaagaggaac
     2701 ttgccgcggg ctctcggccg ggccacttcc gtcgggcgcg cctcgccccg gggcagcaca
     2761 cgttccccgg tcgtcgcgac caggccgtgc gccatagtct ccacggaatc gacggagtag
     2821 gcccgcgcca tcgctcgttg cttcgtaggc cctccgtggt ggccgaacac ctacttcctc
     2881 cctcgggtgg cgaggtctga ctcaatggtg ccccgcaaca catcgcaact cggatgccgt
     2941 ccgcaatccc aagcgccacc atgagttggc gcaccggccc gcaccatttt cggatgtgtc
     3001 gcagttgaga ggtgccccgt tttgccgcat cgcgatcgcg cgtggtggcg caccgccggg
     3061 cgttgagcgc gcgcgactcg cacgcgatcc caacgcgtcg cgacggatga cacgagccgc
     3121 tccgcgccgg ctcgcgcgcc gtggcgtgtc gcgcgacgta gcgagcttca ttttggtgcc
     3181 gcgcgcgtca cgcgcggcac caagattgtc ggcgtcgatc cggtgagaac gacccgatcc
     3241 ccttgcattc cctcgcgata accgcttgca ccgatgtgga acggcccttg ccttctcacg
     3301 cgccggcgat gttcgctgca acgccaccac cttcggccga gggccaccgt gtacgaacgg
     3361 agaggtgccc atccatgacc acgtaccgct cgacgcaggt tcgcacgctc ctgctttacg
     3421 cgcaggacac ccgtggcttg gggcacatca cgcgtaccct ggccatcacc cggcacgtgc
     3481 tggacgcgta ccccgacgtg gtcgcttaca tcgcgacgcg gtcccacttc gcccattcct
     3541 atacgctgcc cgagcggtgc gattacatca aactcccctc gcggcagact ccgtggcaca
     3601 tcgagcggcc acccgaggac gaccaggaga gtaaggagca cttccgccgg cttcgcggca
     3661 tcgtgctgcg ggacgtggcg ttgggactgg ccccggatct ggtgctggtg gatcacgagc
     3721 cgctgggcac caacgccgaa ttcaaagccg ggctctgggc cctgaaggag gagagccccc
     3781 acacccggtt cgtgttcggc ctgcgcgaca tcatggacga cccggagcgg atccgggccg
     3841 aatggcgcga gatgggcgtc tacgaggcgc tggaagggct gttcgacgga atcaccgtgt
     3901 acggctcacc cagcctcttc gatccggtca aggcctacgg gattccggca tcggtccgcc
     3961 acaaactgca ctactgcggc tacgtggtgc gcgacccccc tgaggtcgac tgcgccgccg
     4021 tgcgccgtca gtacggattg cccgaggacg gccagctggt gctggccacg gtgggcagcg
     4081 gctacgacgg gtatccggtg ctcgacgtcg cgctgtcggc gctggaacgc gtgcaagcca
     4141 cgcggccggg attgcatgcc atcctcgtca cggggccgtt catgccaccc cagctgcagg
     4201 cggcgctgca ggcgcgggcg aacggtttct gccgcgtggt gacgaccgcc gacacgttcc
     4261 acctgatggc cgcggccgac gccgttgtga gcatgggggg ctacaacagc gtgtgcgagg
     4321 ggctctcggt ggggcgccca ttggtcatcg tgccgcgcgc cacccacaag gtcgagcaga
     4381 agatgcgcgc cgagttgctc gccgaccagg gcctggccaa gtgcgtgttg cctcaggctc
     4441 tcacccccga gagcgtagag ggcgccttgg actgggctct ggccgtcgat cccgaacttc
     4501 acacgcgccg ggtgcgcgcc gtgctccccg cattcgacgg ggcggcgcgg ctcaccggtt
     4561 acctgtcaca ctggctcggc aagagccgct tcgaggacca ttcccttgcg cacgcgcttg
     4621 aagcccacgg ctgaaccggc ccctgccctg cgtcgccggc ctgcgcgccg gcacgtggcg
     4681 gcaccccctc cactcctgag tgtgtcggtc ccctccccgg ccgctcccac ggtgctgccc
     4741 cggcgggtga gctccaaggt catccgctat gccggggatg cggaccggtt ccggctggaa
     4801 gagtggacca cggcgtgggg cgacggcgcc gcgaccaagg actgcgtctg ggtacgcggg
     4861 ggttacgcgc tggtggtggc cgtcgcgaac gaccgggtgg tgtgcatccg ccagtacaag
     4921 aaggccgccc agcaggtgct gctcgctctg ccctggggcg ggatcgaacc cggggaatcc
     4981 cccctcgatg ccgggcggcg cgagctcgaa gaggaaaccg gcttcagctt cggcgccgcg
     5041 gaggtgcacg gccccttcta cgaccttccg gacaagtcca ccgggggaca ctgggtgatc
     5101 gtggcccggc gcgcattccg caaggccgcg gccgccccgg acgcgggcga gctgatcttc
     5161 ggtaccgaat tgatccccct gcgccggctg tggcagcacc gcttccccgt gctgatgcac
     5221 gtgggcgcgc tgcgcctcgc ccgcctctga tcggggacgg cgccatgcgt gcgcgcttca
     5281 agcccactcc ggagcaacgc tccgccctgc gcgagcagct cactcgtgtg cagcagcccg
     5341 acacggcgct gcggattctc cgccccctgc tgccggaaga cttcgcaccg gcgcacgttg
     5401 cggtgaccct gcagagcgcc catccggacc gcttcgtggt tcgagccgag tgtcgctccg
     5461 tcgatggggg gacatccgta tacgccctga aggcctactg cgacgatttc gtccaccagg
     5521 tctggaatta cagccgagcg ctggcgctgg tccaccggcc caatcaccgc ggattgtgcc
     5581 tgccgattcg ctgcatcccc gaagaacgga tcctcatctc cccctgggtg cagggtgagt
     5641 tcctgtcgga tgtcgtggac gagcgcaggc tgcagctgtt gcgccaggcc gccctgctcg
     5701 ccgcggacgt gcaccgtctc ccgctcgtac ccgaggagct caccagcgcg gagatgttcg
     5761 tcgccgatac gctggcgcga tgcgagcgcc tccgctccaa gtggcctgag gccggctcgc
     5821 tcgtcctgcc gttgatggac gccctggtac gggccagcgc ctgcctcgag ccggccgaac
     5881 ccggaccggt gcatggggac atgtcgcccg ggcagttcct gtggaccggc gagcggctgg
     5941 cgctgctcga tatcgacatg ttcggctacg ccgatccggc gtacgacgtc ggccacttcc
     6001 aggcgcaagt ggagcgccgc tgcatcgtgg acctgacggc gcgaccactc gcgaacacgt
     6061 ggctcgcctg cttcgaggag acgtaccgct cggcgatgcc gcgcgtgtcg ccgcgcaaca
     6121 tcgcgttcta ccgcggactc acgctggtac gcaagatgta cacggtgtgc cgacgcga
//
LOCUS       DBNGZW010000708         2795 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_339446, whole genome shotgun sequence.
ACCESSION   DBNGZW010000708 DBNGZW010000000
VERSION     DBNGZW010000708.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2795)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2795)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2795
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_339446"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="AC16JC_16705"
     CDS             complement(<1..558)
                     /locus_tag="AC16JC_16705"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5166393.1"
                     /translation="MTLAHGAERLESARLVLRRVTPDDLPFFTRIHALPDVARHLYPG
                     GRPRSPEETAEWLRYTLASYEQLALGYLAVLRKEDGALIGRCGLMDLVVESAAPEHGI
                     RRGWFGREQAPAGVPLIFECELGYTFDPAVWGQGFATEAACCVRDYARDVLRLSYAIS
                     AIVPQNARSRRVAERSGARVVGQMDV"
     gene            694..1488
                     /locus_tag="AC16JC_16710"
     CDS             694..1488
                     /locus_tag="AC16JC_16710"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR5166394.1"
                     /translation="MKTQLLAALLALSIATPALAAIKEEPVTYKDGETTMKGVVVYDT
                     ATAGKRPGIVMVHEWWGITKHVHNEARRLAGQGYTVFIADMYGDAKTADNPKDAGALS
                     SSVMKNPQTMQSRFNAAREQLAKQPSVDATRIGAVGYCFGGAVVLNMARAGADLAGVA
                     GFHASLGLNTPAPAPGAVKAKILVLNGADDPFVKKEQYVTFKSELDAAKADYKVIEYP
                     GAVHAFTNPEATALGEKFNLPLRYDAKANEEAKAEASKFFAEIFKK"
     gene            complement(1492..2385)
                     /locus_tag="AC16JC_16715"
     CDS             complement(1492..2385)
                     /locus_tag="AC16JC_16715"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR5166395.1"
                     /translation="MATAGVLGFVGLGVMGEPMCRNLTRKSGATVVAFDERPTPLAAL
                     AADGVQAAASVAEVARRAEIVFVCLPGEPQVRAVCLGAGGGLAALVEPGQTVVDMSTC
                     PVALARELHHAFGARGAAFADAPIARTAQAARDGTLSIMVGGEPALFTRLRPLLGCMG
                     SEVTHCGGVGAGQAVKLMNNMVVAQTVVALAEALAVARATGAVEPRVLFETLAKGSAD
                     SFVLRNHGMKSLLPDHHPTEGAFPTRYILKDLSYAIQLAESAGVTLEQAATTRRLLEA
                     TAAAGWSEAYYTAVIRTIEKR"
     gene            complement(2570..>2795)
                     /locus_tag="AC16JC_16720"
     CDS             complement(2570..>2795)
                     /locus_tag="AC16JC_16720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR5166396.1"
                     /translation="MATVLLTASGLSFLGLGVQPPSPKWGAMLSNARSYMITAPHVAT
                     VPGLAIMLVVLGFNLLGDGLRDSLDPRLRE"
BASE COUNT          464 a          962 c          976 g          393 t
ORIGIN      
        1 cacgtccatc tggccgacga ctcgcgcacc cgaccgttcg gcgacgcgtc gtgagcgggc
       61 gttctgcgga acaatggccg agatcgcgta cgacagccgc aggacgtcgc gcgcgtagtc
      121 gcgaacgcag cacgccgctt cggtcgcgaa gccctggccc cagacggcgg ggtcgaacgt
      181 gtagccgagc tcgcactcga atatcagcgg gacgccggcg ggcgcctgct cgcgcccgaa
      241 ccatcctctg cgaatcccgt gctcgggtgc cgccgattcg accacgagat ccatcaggcc
      301 gcagcgcccg atcagcgctc cgtcttcctt gcgcaggacg gcgagatagc cgagcgcgag
      361 ctgctcatag ctggcgagag tgtaccgcag ccactcggcc gtctcttcgg gggagcgcgg
      421 ccgaccgccg ggatagagat gccgcgcgac gtccgggagc gcgtggatgc gagtgaagaa
      481 cggcagatcg tccggggtga cccgccgcag cacgaggcga gccgactcca gccgttcggc
      541 gccgtgggcc agggtcatcg ggaaggccct ttcatggcgc tcgcattgta gcgccggtcg
      601 cggtgttcct cttgacgccg ggttgtccgc ggtgtattga gggtgggcag tcgtcctcac
      661 cgcgcgccac actccgaaaa gacaggagac gccatgaaga cgcagctcct agccgcgttg
      721 ctcgccctct cgatcgccac gccggcgctg gccgccatca aggaagagcc ggtcacttac
      781 aaggacggcg agaccaccat gaagggcgtc gtggtctacg acaccgccac ggccggcaag
      841 cgtcccggca tcgtcatggt gcacgaatgg tggggcatca cgaagcacgt gcacaacgaa
      901 gcgcgcagac tcgccggcca gggctacacc gtcttcatcg ccgacatgta cggcgatgcc
      961 aagacggccg acaacccgaa ggacgccggt gcgctgtcga gctcggtcat gaagaacccg
     1021 cagacgatgc aatcgcgctt caacgccgcg cgcgagcagc tcgccaagca gccgagcgtc
     1081 gacgccacgc gcatcggcgc ggtcggctac tgcttcggcg gcgcggtggt gctcaacatg
     1141 gcgcgcgccg gggccgatct ggcgggcgtc gccggcttcc acgcctccct gggcctcaac
     1201 acgcccgcgc ccgcgcccgg ggcggtgaag gcgaagatcc tcgtcctcaa cggcgcggac
     1261 gatccattcg tgaagaagga gcagtacgtg acgttcaaga gcgagctgga cgcggccaag
     1321 gccgactaca aggtgatcga atatcccggc gcggttcacg ccttcacgaa ccccgaggcg
     1381 acggcgctcg gcgagaagtt caacctgccg ctgcgctatg acgcgaaggc gaacgaggaa
     1441 gcgaaggccg aggcgtcgaa gttcttcgcc gagatcttca agaagtaggc cctaccgctt
     1501 ctcgatggtg cggatcaccg cggtgtagta ggcctcgctc cagcccgcgg cggcggtggc
     1561 ttcgagaagc cgccgcgtgg tggccgcctg ctcgagcgtg acccccgcgg actcggccag
     1621 ctggatggcg tacgacaggt ccttcaggat gtatcgcgtg gggaaggcgc cctccgtggg
     1681 gtgatggtcg ggcagcagcg acttcatccc gtgattccgg aggacgaagc tgtcggcgga
     1741 ccccttggcg agcgtctcga acaggacgcg cggctccacc gcaccggtgg cgcgggccac
     1801 cgcgagggcc tcggcgaggg cgaccaccgt ctgggcgacc accatgttgt tcatgagctt
     1861 caccgcctga cccgcgccga cgcctccgca gtgggtcacc tccgagccca tgcacccgag
     1921 gagcgggcgg agccgggtga agagcgccgg ctccccgccc accatgatgc tgagcgtgcc
     1981 gtcgcgggcg gcctgggcgg tgcgcgcgat tggcgcgtcg gcgaaggcgg cgccgcgcgc
     2041 gccgaaggcg tgatggagct cccgcgcgag cgccaccggg caggtgctca tgtcgaccac
     2101 cgtctggccc ggctccacga gggcggcaag gccgccgccg gcgccgaggc agaccgcgcg
     2161 gacctggggc tctcccggca ggcacacgaa cacgatctcc gcgcggcgcg ccacctcggc
     2221 caccgaggcc gcggcctgca cgccgtcggc cgcgagcgcg gcgagcggcg tcggccgctc
     2281 gtcgaaggcc accaccgtgg ctccggactt tctcgtcagg tttcggcaca tcggctcgcc
     2341 catgaccccg agtccgacga atccgagcac tcctgctgtc gccatgcctg cctcccatgc
     2401 tccgattgct tcggcacgct ctagaagaac ctcgactgtg ggtcaagcgg gggtcggcgt
     2461 caagcgcgtg cccgggatgt agcttgtcgc cagaagcttt cggctaacct ttgcgcagca
     2521 tgctcatctg acgatggtca cgcatggcgc acgagacgga cggtgggcgc tactcccgca
     2581 ggcgcgggtc gagggagtcg cgcaggccgt cgccgaggag attgaagccg agcaccacga
     2641 gcatgatggc cagccccgga acggtcgcca cgtggggcgc cgtgatcatg taactccgtg
     2701 cgttcgagag catggcgccc cacttggggc tcggcggctg gacgcccagt cccaggaagg
     2761 agaggccgga ggccgtcagc agcaccgtgg ccatc
//
LOCUS       DBNGZW010000709         3379 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1701424, whole genome shotgun sequence.
ACCESSION   DBNGZW010000709 DBNGZW010000000
VERSION     DBNGZW010000709.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1701424"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            456..716
                     /locus_tag="AC16JC_16725"
     CDS             456..716
                     /locus_tag="AC16JC_16725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166397.1"
                     /translation="MSPPRSLDTTREADAIRFEGYARMSPAEKARRVADLTRTACTLA
                     LAGLRDRYPKADEAELLLRLAVLRLGARTVSEAYGWRAPDDA"
     gene            739..1290
                     /locus_tag="AC16JC_16730"
     CDS             739..1290
                     /locus_tag="AC16JC_16730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166398.1"
                     /translation="MALAASGILDRLGIPYVITGSFASSVHGEPRSTDDVDVVADVRP
                     VHVSPLTAALAAEWYVSEQAMREAIDAGTSFNAIHLPTGVKVDIFVVASDPFDAQRVA
                     AARLVRIGQPAAVLRIDAAEYILLRKLEWFRRGGETSDRQWRDVLGIIRTQGTRLDRA
                     ELATWADRLGVADLLARALREAG"
     gene            1402..2289
                     /locus_tag="AC16JC_16735"
     CDS             1402..2289
                     /locus_tag="AC16JC_16735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5166399.1"
                     /translation="MQLNAIITLALAAGLLAAPAIADAQAVGPRAVGRRVQVHGMRMY
                     YEVSGRGAPLIVLHGAYMNIPSMGAIIPGLARTHRVYALELQGHGRTTDIDRPITYPD
                     MADDVAAFMEAVGLPRADVFGYSMGAGVGLQLAIRHPANVNKLVVASAAYDTAGLQPE
                     FKALIPQMTVEMFLQTPFAAQYRKLSPTPDSFPSLVRKLIALEHEPISWGEDLKRVTT
                     PVLIIAGDADGYTLEHIVSMFRLLGGGAMGDMGKPLPASRLAILPATSHTAVITQVDL
                     LLGFIEPFLKGVTPKGFVQ"
     gene            2340..2987
                     /locus_tag="AC16JC_16740"
     CDS             2340..2987
                     /locus_tag="AC16JC_16740"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012373180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR5166400.1"
                     /translation="MARVRVHHFSISIDGYGAGPHQSTKDPLGIGGESLHEWFIPTRT
                     FKQAHGGEGGSTGVDDDIAARGFTGIGAWIMGRNMFGPVRGPWPDQNWRGWWGENPPY
                     HTPVFVLTHHPRPPLALNGGTVFHFVTDGIEAALKRAREAAGGKDVRIGGGAATIRQY
                     LEARLVDELHVAVAPILLGSGESLLAALDLPALGYRCTEHVATDHATHYVIARAR"
BASE COUNT          534 a         1162 c         1134 g          549 t
ORIGIN      
        1 tcggagtgcg cctcggcttc taggtcccga ttctcgcgtg tgatccaccc tgtgagttcg
       61 gcccctgggc agcatccctg gctgagctaa gtagtgaccc cccgctgcag cagttgtgtt
      121 cggggtcggc tagggcctgc ggcaaacatt ccggcaaagc gccttctact cacacaagca
      181 gagccggtta gcgccgcgca acggatgacc aatccgccgg cctcctgatg agtcgatctg
      241 ggtatcgacg gtccgtgcac cgaggctggc accgggagcg actaacccgt gacagcgccg
      301 gactgaactg tggctcgcat ggcgggggag aaggggatga agaccatgca tctggtgccc
      361 cctctccgcg tcaagcggta agggggacag ggggagaggg acagaccgat ccgccgaacg
      421 tggctccccg ccaagccgca ccgcatattt gacccgtgtc cccaccccgc tcactcgaca
      481 ccacccgcga agccgacgcc attcgcttcg agggttatgc ccgcatgtcc ccggcggaga
      541 aggcgcgccg cgtcgcggat ctgacacgca cggcgtgtac gctcgccctg gccggtctcc
      601 gggaccggta ccccaaggcg gacgaggccg agctgttgct gcggctggcg gtgctccgcc
      661 tcggcgcccg gacggtcagc gaggcctacg ggtggcgggc cccggatgac gcctgaggcg
      721 ctacccgatc ctctccgcgt ggccctcgcc gcctccggca ttctcgatcg actcggcatc
      781 ccctatgtga ttacaggctc gttcgccagc tccgtgcacg gcgagccgcg atccactgac
      841 gacgtcgacg tggtagcgga tgtacggccc gtgcacgtct cacccctgac cgccgcactc
      901 gccgccgagt ggtacgtgag cgagcaggcg atgcgcgagg cgatcgacgc gggcacgtca
      961 ttcaacgcca ttcatctccc caccggcgtc aaggtcgaca tcttcgtggt cgcgagcgat
     1021 cccttcgatg cgcagcgcgt cgctgccgcg cggctggtgc gcatcggcca acccgccgcc
     1081 gtgctccgga tagacgccgc cgagtatatc ctcctgcgaa agctcgaatg gttccgccgt
     1141 ggcggggaga cgtccgaccg ccagtggcgt gacgtgctcg gtatcattcg gacccagggc
     1201 acccggctcg atcgagcgga gctggcgacg tgggccgacc ggctgggtgt tgcggatctg
     1261 ctcgcgcgag cgctccgcga agcgggctga cccacccccg cgtgtcgatt cccgctcggc
     1321 tcattcgacg tccagatgag accactgtcc gaccaaccag gagacgccat gaccgccgta
     1381 ccggctgaca ccgcgcccag gatgcagctc aacgcgatca tcacgctggc actcgcggcc
     1441 gggttgctcg ccgctcctgc cattgccgac gctcaggcgg tgggccctcg cgccgtgggc
     1501 cgccgcgtgc aggtccacgg catgcggatg tattacgaag tctccggccg gggcgctccg
     1561 ctcatcgtgc tgcacggcgc gtatatgaac atcccgtcca tgggcgcgat catccccggg
     1621 ctcgcccgga cgcaccgggt gtatgcgctc gagctgcagg gccacggtcg taccacggac
     1681 atcgaccggc cgatcaccta ccccgacatg gccgacgacg tcgcggcgtt catggaagcg
     1741 gtggggctgc cgcgggccga cgtgttcggc tattcgatgg gcgcgggcgt ggggctgcag
     1801 ctggcgatcc gtcacccggc caacgtgaac aagctcgtcg tggcgtcggc cgcctacgat
     1861 accgcagggc tccagcccga gttcaaggcg ctcatcccgc aaatgacggt ggagatgttc
     1921 ctccagacgc cgttcgcggc gcagtatcgc aagctctcgc ccacaccgga cagcttcccc
     1981 tcgctggtac ggaaactgat cgcgctcgag cacgagccca tctcgtgggg cgaggacctg
     2041 aagcgggtga cgactccggt gctgatcatt gcgggggacg cggacgggta cacactcgag
     2101 cacatcgtct cgatgttccg cctgctcggc ggcggtgcca tgggcgatat gggcaagccg
     2161 ctgccggcct cgcgcctcgc gatcctgccg gcgacctcac acaccgccgt catcacgcag
     2221 gtcgacctgc tgctggggtt catcgagccc ttcctgaagg gtgtgacgcc gaaaggcttc
     2281 gtccagtagt ccaggaggta tgagcttgag gtcggcacca atacctgcgg aggcgggtta
     2341 tggcacgtgt acgcgttcac catttcagca tttcgatcga cggctacggc gcgggaccac
     2401 accagtcgac caaggatccg ctgggcattg gcggcgagag cctgcacgaa tggtttattc
     2461 cgacgcggac cttcaagcag gcgcacgggg gcgagggcgg cagcacgggt gtcgacgacg
     2521 acatcgcggc acgcgggttc actggcattg gcgcctggat catgggccgc aacatgttcg
     2581 gccccgtgcg cggaccatgg cccgatcaga attggcgtgg ctggtggggt gagaatcctc
     2641 cgtatcatac gcccgtgttt gtgctcaccc accacccgcg cccgccgctg gccttgaacg
     2701 gcggcacggt gttccacttc gtcaccgatg gcatcgaagc ggcattgaaa cgtgcgcgcg
     2761 aggcggccgg cggcaaggac gttcgcatcg gcggaggggc ggcgacgatt cgtcagtatc
     2821 tcgaggcccg gctggtggac gagttgcatg tcgccgtggc gccgatcctg ctcggcagcg
     2881 gggagtccct gctggccgct ctcgacctcc cggctctcgg gtatcgctgc accgaacacg
     2941 tcgcgactga ccacgcgacg cactacgtga tcgcgcgggc caggtaaccg gcaccgcggg
     3001 tcaggccgca ccaagccctc acctggaaac gtctcctgcc ggcgcagatc atcgaaaagg
     3061 ccgcggcgat cctgggcgtg gaggctactg aggcccgctg tcacagccgg gtggtatcga
     3121 agggagacca aggagagtca ccatgaacct cggtcgcccg ttgcgcatct tcgagaacta
     3181 agggtgggcc gcaaccccca gcgccgccgg cgggccggcc ccctcccgtc gtgacggcca
     3241 gcgcgcggcc ggctgtggtg accccggcgc ctcgcccgat gccgattctt cccggtcccc
     3301 gtcccggctc cgtcacggcg ccgggcgacc gggaaatccc ggtggcgaag tgggtggagg
     3361 cgcgcgagct gctcgcgtg
//
LOCUS       DBNGZW010000710         4326 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_271987, whole genome shotgun sequence.
ACCESSION   DBNGZW010000710 DBNGZW010000000
VERSION     DBNGZW010000710.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4326)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4326)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4326
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_271987"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(3..1232)
                     /locus_tag="AC16JC_16745"
     CDS             complement(3..1232)
                     /locus_tag="AC16JC_16745"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004130 - cytochrome-c peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c peroxidase"
                     /protein_id="HIR5166401.1"
                     /translation="MKSSRLAVGITLLGAAVACTDNRVPVSPPQANLLESALQSNTAD
                     LAPLGKLIFFDQNLSLNDNQSCASCHAPEVGWTGALSSTNAHGAVYEGSIPGAFGARK
                     PPSSAYATQSPILNLSGVKKHAVFTGGMFWDGRATGEILGSPTAEQAKGPFLNPVEQA
                     LPNAADLVGRVCGASYGDDFRAAWGASICDPANVDQAYDAIGYSVSAFEGSSEVNAFT
                     SKFDAFRAGQATLTREERRGWALFNGQAKCAGCHVGNGEHALFTDYTFDNIGLPQNPE
                     NPAGLGFFDPGLGGFLEQRADYSSYATANLGKHKVPTLRNVDLRPSPDFVKAYGHNGY
                     FKSLYGIVHFYNTRDVLETCPGNYTEAQALAADCWPGPEISQNLNTAELGNLGLTLSQ
                     EQAIVAFLKTLSDGYEP"
     gene            complement(1355..2737)
                     /locus_tag="AC16JC_16750"
     CDS             complement(1355..2737)
                     /locus_tag="AC16JC_16750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012615731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5166402.1"
                     /translation="MTATGEGSRGRILLLLFGGVLMGALDIAVVGPALPAIRESFGID
                     ERAAAWLFTIYVLANLVGTPLMAKLSDRFGRRALYVTDTALFGVGSLIVALAPDYGML
                     LAGRAIQGFGAGGIFPVAAAVIGDTFPPERRGGALGLIGAVFGIAFLIGPMLGGLLLP
                     FGWQWLFVVNLPTAAAVILDSMRLLPGGRPAVEARFDWAGMAVLGAALTALAYGVSTI
                     DVERFAISVLSARVWPFLLIAVGLVPVFVLAERRAADPVLRLSLFRSSQVGITAGLAV
                     GAGLGEAASVFVPAFLVSAFGVRTSTASFMLLPVVVALAVGAPVWGRLLDRVGARVVV
                     VVSTVLIALGMLVFATMGTALTGYYVGAVLGGLGLAGLLGSSLRYLMLNSTPAADRAS
                     AQAALTIFMSVGQLLGGAAVGAIVASAGGGVPGFDRAFAAIGIVALALALVALGLKGR
                     REELAAAAPH"
     gene            2774..3049
                     /locus_tag="AC16JC_16755"
     CDS             2774..3049
                     /locus_tag="AC16JC_16755"
                     /inference="COORDINATES: protein motif:HMM:NF025715.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HIR5166403.1"
                     /translation="MDQSQLHRLLGELHQELLAAHSVDPKSRDLLQHLASDIRAIVDT
                     PSHQASPEHYQGLRGRLTEAVAAFEASHPRLTTAMERVIDTLALSNL"
     gene            complement(3046..3741)
                     /locus_tag="AC16JC_16760"
     CDS             complement(3046..3741)
                     /locus_tag="AC16JC_16760"
                     /inference="COORDINATES: protein motif:HMM:NF018021.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase family protein"
                     /protein_id="HIR5166404.1"
                     /translation="MDDFRALVRRLQADIPEQFQGGVVEIEVSPKTVPHPLRGDVYTL
                     GECIPLEWSGSGADLQSRVVLYHGSFQALSRLGDFDWREEAWETLTHELRHHLEWQAD
                     VASLEAYDWAAEQNFARHEREPFDPVFYRNGEQLGPGAFKVDDDVFIEAEQGARSREQ
                     VDVSWHGRRYRVRLPPAAERRTWVFVTLEGLDEPPPGDAVLVFPGRSSMLDLFGRVPQ
                     PESRAAVVERLAG"
     gene            complement(3853..>4326)
                     /locus_tag="AC16JC_16765"
     CDS             complement(3853..>4326)
                     /locus_tag="AC16JC_16765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HIR5166405.1"
                     /translation="QHSGKKLEYVDTGTNERFVPYIIETSAGADRTALAVLADAYHAE
                     EVAGEKRVVMRFPPALAPIKAAVFPLVNQDGMPELAHRIYEAVRRHVNTFYDDGGSIG
                     RRYRRQDEAGTPFGITIDGQSTQDQTVTVRDRDTLLQERVGADRLVDYLHAKITG"
BASE COUNT          754 a         1526 c         1412 g          634 t
ORIGIN      
        1 tcctacggct cgtagccgtc gctcagcgtc ttgaggaagg ccacgatcgc ctgctcttgc
       61 gagagcgtga gaccgaggtt gcccagctcg gcggtgttga gattctgcga aatctcgggc
      121 ccgggccagc agtccgcggc cagcgcctgt gcttcggtat agttcccggg acatgtctcg
      181 aggacgtccc gcgtgttgta gaagtggacg attccgtaca gggacttgaa gtagccgttg
      241 tgcccgtacg ctttgacgaa gtcgggagag ggccgcaggt ccacgttgcg cagcgtgggc
      301 accttgtgtt tgccgagatt cgccgtggca tacgacgagt aatcggcgcg ttgctcgagg
      361 aaaccgccca gaccggggtc gaagaacccc aggccggcgg gattctccgg gttctgcggg
      421 aggccgatgt tgtcgaaggt gtagtccgtg aacagggcgt gctctccgtt tcccacatgg
      481 cagccggcgc atttcgcctg tccattgaac aatgcccatc cgcgccgctc ctcgcgggtg
      541 agcgtagcct ggcccgcccg gaaggcgtcg aacttcgagg tgaaggcgtt gacctccgac
      601 gatccttcga acgccgagac cgagtaaccg atcgcgtcgt acgcctggtc gacgttggcg
      661 gggtcgcaga tcgaggcgcc ccatgccgcc cggaaatcgt cgccgtagga agcgccgcag
      721 acgcggccta ccaggtccgc cgcgttcggc agagcctgtt cgaccgggtt cagaaacggg
      781 cctttggcct gctcggccgt ggggctgccg aggatctccc cggtcgcgcg gccgtcccag
      841 aacatgccgc cggtgaacac ggcgtgcttc tttacgccgc tgagattgag gatcgggctc
      901 tgcgtcgcat aggcggagct gggcggcttg cgggcgccga aggcgccggg aatggagcct
      961 tcgtaaacgg cgccatgtgc attggtggat gacagcgctc cggtccagcc tacctcgggg
     1021 gcgtggcacg aagcgcacga ctggttgtcg ttcagtgaca gattctggtc gaagaagatc
     1081 aactttccca atggggccag atccgccgta ttcgactgca gtgcgctttc caacagattg
     1141 gcttgcggtg ggctgaccgg tactcgattg tcggtgcagg ccacggccgc gccgagcagc
     1201 gtgatgccga ccgcaaggcg tgaggatttc atggatgcct ccttgtcgca ggacggactt
     1261 cgccgatgcg gtcaggctag gggaggcgtc gtgaatcggg agtgagaacg gctggaagag
     1321 agcgcaccct ggcccatgaa gccgggatga agggtcaatg cggagcagcc gcagccagct
     1381 cctccctgcg ccccttcaac ccgagcgcca cgagcgccag cgcgagcgcc acgattccaa
     1441 tggccgcaaa ggcgcgatcg aatcccggaa ccccgccgcc ggctgaagcg acgatcgccc
     1501 ccactgccgc cccgcccaac aactggccca cgctcatgaa gatggtcagg gccgcctgcg
     1561 ccgatgcgcg gtcggcggcg ggagtggaat tcaacatcag gtagcgcagc gacgacccca
     1621 gcagccccgc cagcccgagc ccgcccagca ccgcgccgac gtaatagccc gtgagtgctg
     1681 ttcccatcgt ggcgaaaacc agcattccca atgcgatcag gaccgtgctc accacgacga
     1741 cgacgcgggc ccccacccgg tccagcagcc gcccccacac cggcgcaccc acggcgagcg
     1801 ccacgacgac cggcaggagc atgaagctcg ccgtggacgt gcgcacaccg aacgccgaca
     1861 cgaggaatgc cggaacgaac acggacgccg cctccccgag ccccgccccg acggcaaggc
     1921 ctgcggtgat ccccacctgg gaagagcgaa acagggagag ccgcagcacc ggatccgccg
     1981 cgcgacgctc ggcgagcacg aacacgggca ccagccccac ggcgatcagc agaaacggcc
     2041 atactctggc cgacaggacc gagatcgcga aacgctcaac gtcgatggtc gacacgccgt
     2101 acgcgagggc ggtgagggct gcgcccagca ccgccatccc cgcccagtcg aaccgcgcct
     2161 ccacggcagg tctgccgccg ggcagcagcc gcatgctgtc caggatcacc gccgccgcag
     2221 tcggcaggtt caccacgaac agccactgcc acccgaacgg caacagcagg ccgccgagca
     2281 tcggtccgat gaggaaggcg atcccgaaaa cggcgccgat cagtcccagc gccccaccgc
     2341 gccgttcggg cgggaaggtg tcgccgatga ccgctgcggc gacggggaag atgccgccgg
     2401 caccgaaccc ctggatcgcc cgcccggcga gcagcatccc gtagtcgggg gccagcgcga
     2461 cgatcagcga cccgactccg aacagcgccg tatccgtcac gtacagcgcc cggcggccga
     2521 agcggtccga caacttggcc atgagcggcg tgccgaccag gttggcgagc acgtagatcg
     2581 tgaacagcca cgccgccgcg cgctcgtcga taccgaagct ctcccggatc gccggcagcg
     2641 ccggcccgac gacggcgatg tccagcgccc ccatcaggac gccgccgaac aagaggagga
     2701 ggatcctccc tcgcgatccc tcgccagtcg ccgtcattgg cctcctccgc acgccggacg
     2761 atatcttaca cccatggacc agagccaatt gcacaggctg ctcggggagc tgcaccagga
     2821 gctgctcgcc gcgcactccg tcgacccgaa gagccgcgat ctgctgcagc acctcgccag
     2881 cgacattcgg gcgatcgtcg acaccccatc acaccaggca tcgcccgagc actaccaggg
     2941 gctgcgcggg cgcctgacgg aggcggtggc ggccttcgag gcctcgcatc cccgcctcac
     3001 gacggcgatg gagcgagtga tcgacacgct cgccctctcg aacctctagc cggccagccg
     3061 ctcgaccacc gccgcccgcg attcgggctg cggcacgcgc ccgaacagat cgagcatcga
     3121 cgaccggccg gggaagacca gcaccgcatc ccccggcggc ggctcgtcga gcccttccag
     3181 cgtgacgaac acccaggtgc gtcgctcggc cgcgggcgga agccggaccc gataccggcg
     3241 tccgtgccac gacacgtcga cctgctccct gctccgtgct ccctgctcgg cctcgatgaa
     3301 aacgtcgtcg tcgaccttga aggccccggg ccccagctgc tcgccgttgc ggtagaacac
     3361 cgggtcgaac ggctcgcgct cgtgtcgcgc gaagttctgc tccgcggccc agtcgtaggc
     3421 ctcgagcgag gcgacgtcgg cctgccactc gaggtgatgg cgcagctcgt gggtcagcgt
     3481 ctcccaggct tcttcgcgcc agtcgaagtc gccgagccgg gagagcgcct gaaaggatcc
     3541 gtgatagagc acgacgcggg actgcaggtc ggcgccggag ccgctccact cgagcgggat
     3601 gcactccccg agggtgtaga cgtcgccgcg cagcgggtgg ggaacggtct tgggactgac
     3661 ttcgatctcc accaccccgc cctggaattg ctcggggatg tcggcctgca accgccggac
     3721 cagcgcacgg aagtcatcca agcgcacggc tcggccccgc cccattgcgg attgcggatt
     3781 gcgaattgcg gattgacgat tcgacctcac cctctccaat ccgcaatccg caatccccaa
     3841 tccgcaatcg gttcacccag tgatcttcgc gtgcaggtag tccaccagtc ggtccgcccc
     3901 cacccgctcc tgcagcagcg tatcgcgatc gcgcaccgtg accgtctggt cctgggtgga
     3961 ctggccgtcg atcgtgatgc cgaacggcgt gccggcctcg tcctggcggc ggtagcgccg
     4021 gccgatcgag cccccgtcgt cgtagaacgt gttgacgtgg cgacgcaccg cctcgtagat
     4081 gcggtgcgcc agctccggca tcccgtcctg gttgaccagc gggaacaccg cggccttgat
     4141 cggcgccagt gcaggaggga agcgcatgac cacgcgcttc tcgcccgcca cctcttccgc
     4201 gtgataggcg tccgccagca cggcgagcgc cgtgcggtcg gcgccggccg aggtttcgat
     4261 gatgtagggc acgaaccgct cgttggtccc cgtgtccacg tactcgagct tcttgcccga
     4321 gtgctg
//
LOCUS       DBNGZW010000711         8347 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1754578, whole genome shotgun sequence.
ACCESSION   DBNGZW010000711 DBNGZW010000000
VERSION     DBNGZW010000711.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8347)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8347)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8347
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1754578"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1185
                     /locus_tag="AC16JC_16770"
     CDS             <1..1185
                     /locus_tag="AC16JC_16770"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033840 - alpha-1,4-glucan
                     glucosyltransferase (NDP-glucose donor) activity [Evidence
                     IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase"
                     /protein_id="HIR5166406.1"
                     /translation="FFIEHPDFFDRSGIYGDSAGDYPDNALRFGFFCKAAVAALPAIA
                     PGAQVLHAHDWHTALAPVYLRTVFRGLPFHDGLAIVLSVHNAGFQGHYPRETMRQLGL
                     PEELYGPNGFEWYDRVNILKGGIAFADVVVTVSPTHARELCTPTGGFGLDDTFIALGD
                     RLQGVLNGIDLKAWNPKTDPYIAANYTADDLTGKQRCKAAVQRAYDLPEDDRIPVFAM
                     SARLVGQKGLDLVLGGDLLSSVNAQFVFLGSGEHRYHEALANLAAAHPERVVAEFAFT
                     DRQEHILLAGADLLLMPSLYEPCGLTQMRAQRYGVIPIVRAVGGLQDSVQEGVTGFLF
                     GAYTPAALEEVVEHVLQRYADREGWHVLVRQAMGSRFGWDQSTAQYSALYRRALTTHA
                     QA"
     gene            1172..2842
                     /locus_tag="AC16JC_16775"
     CDS             1172..2842
                     /locus_tag="AC16JC_16775"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein
                     domain-containing protein"
                     /protein_id="HIR5166407.1"
                     /translation="MRKPDAAPVDFVWTLHSHLPYVLNHGRWPHGSDWLCEAALDTYL
                     PLLEQLRALEQEGIPAPVTIGFTPILANQLASPAFGRELEAFFDQRLVACGAAPASLA
                     TTGDQALLPLVDYWKRHLERLRELFRSTGGDLLAAFRELQERGTLEIISSAATHGYLP
                     LLRRDESVRLQLAVGFSEHCRLFGTAPAGCWLPECAYRPRQGDRPGIEEFLEEAGFRF
                     FFVDSHLARAGAPLGAYGDVPLGAERFDAERHETAHGEAVRVAHSPYRAYRVSRTRPG
                     VAALVRDPRSSMQVWSRQQGYPGDEWYLEFHKIRWPGGLKLWRVTGSGVDLGDKRPYE
                     PVRAHERAADHARHFADLLGTIGRAKAGLVVAPFDTELFGHWWFEGVDFLASTYRELR
                     RSPGVRPVTASRHLREHPPEKALRLAEGSWGVNGDHSMWMNEQTRWTWDRLWPLEDAF
                     WDAAPAALTVSAARSVLAQAARSMLLAQASDWQFILSTGAVPDYAERRFNGHCQDAER
                     LLDGLRTGALEGATRLAAELAQRDAVFPDVLEAVAAVVRGRAAAGVLG"
     gene            2860..4860
                     /locus_tag="AC16JC_16780"
     CDS             2860..4860
                     /locus_tag="AC16JC_16780"
                     /inference="COORDINATES: protein motif:HMM:NF023478.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HIR5166408.1"
                     /translation="MVHGHFYQPPREDPWLEAVVREPSAAPAHDWNERIEQECYRAVV
                     AARRYGAEGRIAAVVNTLTSISFDFGPTLLAWLEHAAPATYEAVLAADRESARRHGGH
                     GNALAMPYHHLILPLASRRDKVTEVRWGIADFRRRFRRDPVGFWLPETAVDDETLDVL
                     AAEGIRFTVLAPHQVEGAPAGGLPGSYRTTGGRTIALFVYDGSIANEVAFGSLVRDGG
                     LWAQRMLAPDATGARRPLVSIATDGETWGHHHPFGEVGLAWMLHLLETRDDVRVENFA
                     AVLARRPPEGEVRLVAPSSWSCPHGVERWRSDCSCRIAPDPAGRPPRWRAALREGLDW
                     LAGELHARFELEGAQLLADPWEAREGYAQVEGDPAQVERFVKRRLRSKRHAAARVRAA
                     ELLELEWNALAMFTSCGWFFDDISGIEAQQVLRYAARAIELAGPDAQFLEAGLLERLA
                     RAESSREASGRDLYLTHVKPNLPAPVRVAAGHAAARRLTPRAEIPGVPGYRASQRGGR
                     VTVERRRTGREDTFRVGVERLGGTAVAVEASGGAGGPTRLGLDDLPEGYRVPLVAALR
                     AELVERRLSAVDRARLAAGEAELPRLAALALTRAVAALARDRSAKAIAAVCDLADLLG
                     LLEQHIPFDAQTTFARLRADAGPSADAALAGVGRRLGFAEET"
     gene            4861..6711
                     /locus_tag="AC16JC_16785"
     CDS             4861..6711
                     /locus_tag="AC16JC_16785"
                     /inference="COORDINATES: protein motif:HMM:NF015052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase/4-alpha-glucanotransferase
                     domain-containing protein"
                     /protein_id="HIR5166409.1"
                     /translation="MDPVRLVFGVHFHQPVGNFDHVFEAHVRDVYQPFLERLAEREFF
                     PLALHVSGPLLEWLESHASALVDRIGGLAAEGKVELLLSGFYEPVLISLPRHDRIEQV
                     SWMREAMRRRFGLEPTGLWLTERVWEPELAADLADAGVRYALVDDRHFLVSGFPAERL
                     HQPYWTESDGRRIALLPIHERLRYLVPFKPPTDTVAYLHGLRAARRPLAVLVDDGEKF
                     GGWPGTREWVYERGWLREFLDAVQQTIGAGEVRLTTPAAAVAEVPSGGLAYLPTASYR
                     EMEAWALPPAAATRLAVLEQELGPERLAGPEGALVRGGHWRHFLVKYPEAGRMHRKML
                     ALSGLARRRGDPPAARRAIGRAQCNDAYWHGVFGGLYLPHLRRAVWEQLAAAERVLRR
                     GEPPAVERLDVDGDGVEELWAHSASWSILVSPARGGAIEDLTLLERGVNLADVLTRRR
                     EPYHDPAGNGEGGAVDGEDRVLSVERVLPDALRRESYERGDYVAARTWTREPFTARAE
                     ASAGGAEIVLRAAGLGKRYRFPSSGPILIEYRWDVAALGGGGGWFAPELSLAHPVTVR
                     CVPEAERWEYDLVTVSKSERGLEQTVQGRAITVRWPVAAGEGRIELVLNE"
     gene            6725..7174
                     /gene="msrB"
                     /locus_tag="AC16JC_16790"
     CDS             6725..7174
                     /gene="msrB"
                     /locus_tag="AC16JC_16790"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR5166410.1"
                     /translation="MEFRKPGDEVLRQTLTPRQYDVTQHEGTEPPFQNEFWNEHRAGI
                     YVDVVSGEPLFSSLDKFDSRTGWPSFTRPLETENVREKSDRSLFMERTEVRSANADSH
                     LGHLFDDGPQPTGLRYCINSAALRFIPVERLEAEGYGRYLPLFRKSD"
     gene            complement(7171..7377)
                     /locus_tag="AC16JC_16795"
     CDS             complement(7171..7377)
                     /locus_tag="AC16JC_16795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166411.1"
                     /translation="MALLLGLAWLFLGGRGGTHEPTAHRKVNEEIDYTELEQAERDVR
                     DADDEDSVRDWGPGAGKPRPPHLL"
     gene            complement(7398..>8347)
                     /locus_tag="AC16JC_16800"
     CDS             complement(7398..>8347)
                     /locus_tag="AC16JC_16800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003545530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HIR5166412.1"
                     /translation="HVKVKYPQFEGQTKTKLGNSEVEGIVKSVVNEALGDFLDKNPSV
                     ARAVIAKAVSAARAREAARKARELVRRKNALDSGMLPGKLADCSLTDPSLCEVYLVEG
                     DSAGGSAKQGRDRSFQAILPLRGKILNVQKARIDKILSNEEIRTIITAIGTGVGEDFK
                     IGEARYHKIIIMTDADVDGAHIRTLLLTFFYKEMKQLIDAGYIYIAQPPLYRVAKGKE
                     EFYAYDEKQRDDYVKRLTNGAETKGSVVIQRYKGLGEMNPDQLRDTTMDPPKRTLLKV
                     TMEDALEASTLFETLMGDEVEPRRRFIEENAKFVRNLDV"
BASE COUNT         1143 a         2863 c         3004 g         1337 t
ORIGIN      
        1 ttcttcatcg agcatccgga tttcttcgat cgcagcggga tctacgggga ttccgctggc
       61 gactacccgg acaacgcgct gcgcttcggc ttcttctgca aggcggcggt cgccgcgctc
      121 cccgccatcg cccccggggc ccaggtgctg cacgcgcacg actggcacac cgcgctggca
      181 ccggtttacc tgcgcacggt gttccgcggg ctgccgttcc acgacgggct ggcgatcgtg
      241 ctgtcggtgc acaacgcagg ctttcagggt cactacccgc gggagacgat gcgccagctg
      301 gggctgcccg aggagttgta cggccccaac ggcttcgagt ggtacgatcg cgtcaatatc
      361 ctgaagggcg gcatcgcctt cgccgacgtc gtggtgaccg tgagcccgac gcacgcccgc
      421 gagctgtgca cgccgaccgg cggcttcggg ctggacgaca cgttcatcgc actgggagac
      481 cggctccagg gggtcctgaa cgggatcgac ctgaaggcct ggaacccgaa gaccgatccg
      541 tacatcgccg ccaactacac ggcggacgat ctgaccggaa agcagcggtg caaggccgcc
      601 gtccagcgcg cctacgatct gccggaggac gaccgcattc cggtgttcgc catgagtgcg
      661 cgcctcgtcg ggcagaaggg cctggacctc gtgctgggcg gcgacctgct ctcgtcggtg
      721 aacgcccagt tcgtgttcct gggatcgggg gagcaccgct accacgaggc gctcgccaat
      781 ctcgccgcgg cacatccgga gcgggtcgtg gcagagttcg ccttcaccga ccgccaggag
      841 cacatcctac tggcgggcgc cgacctgctg ctcatgccgt ccctgtacga gccgtgtgga
      901 ttgacgcaga tgcgcgcgca gcgctacggc gtgatcccca tcgtgcgcgc ggtcggcgga
      961 ctgcaggaca gcgtgcagga gggcgtgacc ggcttcctgt tcggcgcgta cacgcctgcg
     1021 gcgctcgagg aagtcgtcga gcacgtgctg cagcgctacg ccgaccgtga ggggtggcat
     1081 gtgttggtgc ggcaggccat gggctcacgg ttcggctggg accagtccac ggcgcagtac
     1141 tcggccctct accgccgcgc gctgacgacc catgcgcaag cctgacgccg cgccggtgga
     1201 tttcgtctgg acgctgcaca gtcatctccc ctacgtcctg aaccacggac gctggccgca
     1261 cgggagtgac tggctgtgcg aggcggccct cgacacctac ctgcccctgc tcgagcagct
     1321 gcgggcgctc gagcaggagg gtatccccgc gccggtgacg atcggcttca ccccgatcct
     1381 cgccaaccag ctcgccagtc ccgccttcgg ccgcgagctg gaggcgttct tcgaccagcg
     1441 cctcgtggcc tgcggcgcag cgcccgcctc gctcgcgacc acgggggatc aggcgctgct
     1501 gccgctcgtg gactactgga agcgccacct ggagcgcctg cgcgagctgt ttcggtccac
     1561 cggcggcgac cttctggccg cctttcgcga gctgcaggag cgcgggacgc tcgaaatcat
     1621 cagctcggcg gcaacgcacg gatatctgcc cctgctgcgc cgggacgaga gcgtccggct
     1681 gcaactcgct gtcggattca gcgagcactg ccggctgttc ggcacggcgc cggccggctg
     1741 ctggctcccg gaatgcgcct accggccgcg ccagggcgac cgtcccggca tcgaggagtt
     1801 cctggaggaa gcagggttcc gcttcttctt cgtcgactcg cacctcgccc gcgcaggggc
     1861 acccctgggt gcctacggcg acgtaccgct cggcgcggaa cggttcgatg cagagcgcca
     1921 cgagaccgcg cacggtgagg cggtcagggt cgcacactcg ccctaccggg cgtaccgcgt
     1981 gtcgcgcacg cgacccggcg tcgccgcgct ggtgcgcgat ccccgttcct cgatgcaggt
     2041 gtggagccgg cagcagggct accccggcga cgaatggtac ctcgaattcc acaagatccg
     2101 ctggcccggc ggtctcaaac tgtggcgggt cacgggcagc ggcgtcgatc tgggggacaa
     2161 gcgcccctac gagcccgtgc gggcgcacga gcgcgccgcc gatcacgccc ggcacttcgc
     2221 ggatctcctg gggaccatcg gccgcgctaa ggcgggcctc gtggtggcgc cgttcgacac
     2281 cgagctgttc ggtcactggt ggttcgaagg cgtggatttc ctggcgtcga cctatcgcga
     2341 gctgcggcgc agtccgggag tccgacccgt gacggccagt cgccatctcc gcgagcatcc
     2401 gccggagaag gcgttgcgtc tggcggaggg atcctggggc gtgaacgggg accacagcat
     2461 gtggatgaac gagcagacgc ggtggacctg ggatcggctg tggcccctgg aagacgcgtt
     2521 ctgggacgcc gcaccggcgg ccctcaccgt ctccgccgcg cgctccgtgc tggcccaggc
     2581 ggcccgttcg atgcttctcg cccaggcctc ggactggcaa ttcatcctct cgaccggcgc
     2641 cgtgccggat tatgccgagc gccgcttcaa cgggcactgt caggatgcgg aacggctcct
     2701 cgacggactg cgcaccggcg ccctcgaagg cgcgacgcgg ctcgccgcgg agctggcgca
     2761 gcgggacgcg gtcttccccg atgtactgga ggccgtcgcg gcggtggtcc gcgggagagc
     2821 cgccgccggg gtgctcggct gaccaaccgt tcctcggtgg tggtccacgg gcatttctac
     2881 cagccccctc gcgaagatcc ctggctcgaa gccgtggtgc gcgaaccctc ggccgcgccg
     2941 gcgcacgact ggaacgagcg gatcgaacag gagtgctacc gggcggtcgt ggcggcccgg
     3001 cgttacggag ccgagggacg catcgccgcg gtcgtcaaca ccctgacctc catcagcttc
     3061 gatttcggac cgacactgct cgcctggctc gagcatgcgg cgcccgcgac gtacgaggcg
     3121 gtcctcgccg ccgaccgcga gagcgcacgc cgccacggtg gccacggtaa cgccctcgcc
     3181 atgccgtacc atcacctgat cctgccgctc gcctcacggc gcgacaaggt gacggaggtg
     3241 cgctggggca tcgccgactt ccggcgtcgc ttccggcgtg atcccgtggg cttctggctg
     3301 ccggagacgg cggtggacga cgagacactg gacgtgctgg cagccgaagg catccggttc
     3361 accgtgctcg cgccgcacca ggtggagggt gcgcccgccg ggggattgcc cggatcgtac
     3421 cgaaccacgg gcgggcggac gatcgccctg ttcgtctacg acggatcgat cgccaacgag
     3481 gtggcgttcg gcagcctggt acgcgacggg ggtctctggg cgcaacgcat gctcgcaccc
     3541 gacgcgaccg gcgcccgccg tccgctggtg tccatcgcga ccgacggcga gacctggggc
     3601 caccaccacc ccttcggcga agtcgggctg gcctggatgc tccacctgct cgagacgcga
     3661 gacgacgtac gggtcgagaa cttcgccgcg gtgctcgcgc gccggcctcc cgaaggcgag
     3721 gtgcggctcg tggcgccgtc gtcgtggagc tgcccgcacg gcgtggagcg ctggcgctcc
     3781 gattgcagct gccggatcgc ccccgatccg gcgggccgtc ccccacgatg gcgcgcggcg
     3841 ctgcgcgaag ggctcgattg gctcgccggc gagctgcatg cgcgattcga gctggagggg
     3901 gcgcagctgc tggccgaccc ctgggaggca cgcgagggct atgcccaggt cgagggcgac
     3961 cccgcgcagg tagagcgctt cgtgaaacgc cgcctgcgat cgaagcgtca cgcagccgcg
     4021 cgcgtgcgcg cggccgagct gctggagctc gagtggaatg cgctcgcgat gttcacctcg
     4081 tgcggctggt tcttcgacga catctcgggg atcgaggcgc agcaggtgct gcgctacgcg
     4141 gcgcgggcga tcgagttggc cgggcccgac gcccagttcc tcgaagcggg cctgctggag
     4201 cggctcgcac gcgccgagag cagcagggag gccagcgggc gggatctcta tctcacccac
     4261 gtgaagccga acctgcccgc ccccgtgcgt gtcgcggcgg ggcatgcggc cgcgcgccgc
     4321 ctcacgccac gggcggagat tcccggcgtc ccgggatatc gcgcgagtca gcgcggcggc
     4381 cgggtgacgg tcgagcgccg tcgcaccggt cgggaagaca cgttccgcgt cggggtcgag
     4441 cggctcggcg gcaccgcggt cgccgtggag gcgtcggggg gcgccggcgg ccccacccgc
     4501 ctcggcctcg acgacctccc cgagggctac cgggtgccgc tggtggccgc cctgcgggcc
     4561 gaattagtcg agcgacggct gagcgccgtg gatcgggcac ggcttgccgc gggggaggcc
     4621 gagctgcccc ggctggccgc cctcgcgctc acgcgggcgg tcgcggcgct ggcgcgcgac
     4681 cggtcggcga aggcgatcgc cgccgtgtgc gacctggccg acctgctggg cctgctggag
     4741 cagcacatcc cgttcgacgc ccagaccacc tttgcgcgat tgcgcgccga tgccggtcca
     4801 tcagccgacg ccgccctggc gggcgtggga cgccggctcg ggtttgccga ggagacctga
     4861 ttggatcccg tccgcctcgt cttcggtgtg cattttcacc agcccgtggg gaacttcgat
     4921 cacgtgttcg aggcccacgt gcgcgatgtg taccagccgt tcctcgagcg gttggccgag
     4981 cgggagttct tccccctcgc cctgcacgtc tcggggccgt tgctcgaatg gctggaatca
     5041 cacgcgtcgg cgctggtgga ccgtatcgga ggactggcgg cggaggggaa ggtcgagctg
     5101 ctgctgtcgg gcttctacga gcctgtgctg atttcgctgc cgcggcacga ccggatcgag
     5161 caggtgagct ggatgcgcga agcgatgcgg cgccggttcg gcctggagcc taccgggttg
     5221 tggctcacgg agcgggtgtg ggagcccgag ctcgccgccg atctcgccga tgccggcgtg
     5281 cgctacgccc tggtggacga tcgccacttt ctcgtgtcgg gatttcccgc cgagcggctg
     5341 catcagccgt actggaccga gagcgacggg cggcgcatcg ccctgctgcc gattcacgaa
     5401 cggctgcgct acctcgtgcc gttcaagccg ccgacggaca cggtcgcata cctgcacggg
     5461 ctccgggccg cacggcggcc gctggcggtg ctggtggacg acggcgagaa attcggcggc
     5521 tggccgggca ccagggaatg ggtgtacgag cgtggctggc tgcgggagtt cctggatgcc
     5581 gtccagcaga cgatcggcgc gggggaggtg cggctcacga cgcctgcggc ggctgtcgcc
     5641 gaggtgccga gcggaggatt ggcgtacctg ccgacggcat cctatcggga gatggaggcg
     5701 tgggccctgc caccggccgc cgcgacgcgc ctcgcggtgc tggagcagga gctcggtccg
     5761 gagcgcctcg ccggcccgga aggagcactg gtgcggggcg gccactggcg tcacttcctc
     5821 gtgaaatacc cggaggccgg ccgcatgcac cgcaagatgc tcgccctctc gggcctggcg
     5881 cgccgccgcg gcgatccccc ggccgcccgg cgcgcgatcg gccgggccca gtgcaacgac
     5941 gcctattggc acggcgtgtt cggcgggctg tacctgcccc atctgcgtcg cgcagtgtgg
     6001 gagcagctgg cggcggcgga gcgcgtgctg cgccgtggcg agcccccggc ggtcgaacgg
     6061 ctcgacgtgg acggcgacgg cgtggaagag ctgtgggcgc actcggcgtc ctggtcgatc
     6121 ctcgtcagcc ctgcgcgtgg cggcgccatc gaagacctga ccctgctcga gcgcggcgtg
     6181 aacctcgccg acgtgctcac gcgccgacgc gagccgtatc acgatccggc ggggaacggg
     6241 gagggcggtg cggtcgatgg agaggaccgc gtgctgagcg tcgagcgggt gctgcccgac
     6301 gcgctgcgac gcgaatccta cgagcgcggc gactacgtcg cggcgcgcac gtggacacgt
     6361 gagccgttca ccgcacgcgc cgaggcgagt gccggcggtg ccgagatcgt gttgcgcgcc
     6421 gcgggcctcg gcaagcgcta tcgcttcccg tcgtccggtc cgatcctcat cgagtatcgc
     6481 tgggatgtgg cggccctcgg aggtgggggc gggtggttcg cgcccgagct gtcgctggcg
     6541 catccggtga cggttcgttg cgtgccggaa gcggagcggt gggagtacga tctggtgacc
     6601 gtgtccaagt ccgagcgggg gctggagcag accgtccagg gtcgcgccat cacggtacgt
     6661 tggccggtcg cggcgggcga gggacggatc gagctggtcc tgaatgagtg agaggagcct
     6721 gccaatggaa ttccgaaagc cgggtgacga ggtgctgcgg cagaccctga ccccccggca
     6781 gtacgacgtc acccagcacg aaggcaccga gccgccgttc cagaacgagt tctggaacga
     6841 gcatcgggcc gggatctacg tggacgtcgt gtcgggggag ccgctgttca gctcgctcga
     6901 caagttcgat tcccggacgg gatggcccag cttcaccagg ccgctcgaga cggagaacgt
     6961 cagggagaag tccgaccgct cgctcttcat ggagcggacg gaagtgcgct cggcgaacgc
     7021 cgattcccat ctgggccacc tcttcgacga cggccctcag cccacggggc tgcgctactg
     7081 catcaactcg gcggcgttgc ggttcattcc cgtggagcgg ctcgaagcgg aggggtacgg
     7141 ccgctacctg ccgttattcc gaaagagcga ctagagcagg tggggcgggc gtggcttgcc
     7201 cgctcccggc ccccagtcgc gcacgctgtc ttcgtcgtcg gcgtcacgca cgtcgcgctc
     7261 cgcctgctcc agctccgtgt agtcgatttc ctcgttgacc ttgcggtgcg ccgtcggctc
     7321 gtgcgtcccg ccgcgcccgc cgaggaacag ccaggccagg ccgagcagca gggccacgac
     7381 cgcgatcgag agggcgatca gacgtcgagg ttcctgacga acttggcgtt ctcttcgatg
     7441 aagcggcgcc gcggctctac ctcgtcgccc atcagcgtct cgaacagcgt cgacgcctcc
     7501 agcgcgtcct ccatcgtcac cttgaggagc gtgcgcttgg gcgggtccat cgtcgtatcg
     7561 cggagctgat cggggttcat ttcaccgagc cccttgtagc gctggatcac cacggagccc
     7621 ttcgtctcgg cgccgttggt gagccgcttg acgtagtcgt cgcgctgctt ctcgtcgtag
     7681 gcgtagaact cttccttgcc tttggccacg cggtacagcg gcggctgcgc gatgtagatg
     7741 tagccggcgt cgatcagctg cttcatctcc ttgtagaaga aggtgagcag cagcgtgcgg
     7801 atgtgggcgc cgtccacgtc cgcgtcggtc atgatgatga tcttgtggta gcgcgcctcc
     7861 ccgatcttga agtcctcgcc gacgccggtg ccgatcgccg tgatgatggt ccggatctcc
     7921 tcgttcgaga ggatcttgtc gatgcgggcc ttctgcacgt tgaggatctt gccgcgcagc
     7981 ggcaggatcg cctggaagct gcggtcgcgc ccctgcttgg ccgagccgcc cgccgagtcg
     8041 ccctcgacga ggtagacctc gcacaggctc gggtcggtga gcgagcaatc ggcgagcttc
     8101 ccgggcagca tgccgctgtc cagggcgttc ttgcgccgca ccagctcgcg cgccttgcgg
     8161 gccgcctcgc gagcgcgtgc cgcgctcacc gccttggcga tcaccgcccg ggcgacgctc
     8221 gggttcttgt cgaggaagtc gcccagcgcc tcgttcacca cgctcttgac gatgccctcg
     8281 acttcggagt tgcccagctt cgtcttggtc tgtccctcga actgcgggta tttcaccttc
     8341 acgtgca
//
LOCUS       DBNGZW010000712         2313 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_678952, whole genome shotgun sequence.
ACCESSION   DBNGZW010000712 DBNGZW010000000
VERSION     DBNGZW010000712.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_678952"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2165)
                     /locus_tag="AC16JC_16805"
     CDS             complement(<1..2165)
                     /locus_tag="AC16JC_16805"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 5 family protein"
                     /protein_id="HIR5166413.1"
                     /translation="MYSSGIRLTHIGAKSRLVLLFSLAIDAGLQAQVANRAVYLDNQG
                     VVRWRDNRQELTLFGANYVLPTASDYRAAGYVHGDRKRMIDEDMAQFARMGWDGMRLT
                     FWGDWESSDSAGNLIANDHLDLLDYLIARARERGIYMLFSPIQLYGANWPDALQDTTD
                     PGFGRRFGRERMGTDSAAIAAQVNYLRQILNHVNPYTHLALKDEPAIIFIELVNEPWH
                     HAEDLAGSIRYINTLTEAVRSTGCSKLVFYNVSQDFRIGEAIRRSKAQGVTFGWYPSG
                     LVSGHELQGNFLRAVDAYPDMLRPELARLPRIVYEFDTPDLRTGYLYPAMARTFRAVG
                     TQFAAMFTYDMQQTASRNLGWQTHYLSLAYTPRKAMSAIIAAEAMHRLPRLRAYGAYP
                     QNTRFGDFHVSYAENLGELVAADAFLYAGSTSSTPPDPAALRRIAGYGSSPTVTYEGE
                     GIYFLDKVRPGVWRLEVYPDAVPVRDPFEPPNPAKIVTRAISRAWPMTVRLPDLGNQF
                     TVQAMNDGGRAVRRSEAPAANGRFTVTPGVYLLSAGGPVDIETLPGQIGHLGLDEFHA
                     PPLDSLPPSVLPLTPPEFLAGREAELRVHVAEVTPPDSVVLFIRPSPGGFYRGFPMRP
                     AGGYDYSAVLPVTVLEEGPHDYVVTLFQGDSRLTFPGGISGRPWDWDYYSRETWNLDV
                     VGARTPLVLFEPARDAGSLAFSRIGDAGRRGLFRLGVSEA"
BASE COUNT          384 a          740 c          816 g          373 t
ORIGIN      
        1 gcctccgaca ctcccaggcg gaacaacccg cggcgacctg cgtcgccgat ccggctgaac
       61 gccaggctcc cggcatcccg ggccggctcg aacaggacca gcggcgtccg cgcgcccacg
      121 acgtccaggt tccacgtctc gcggctgtaa tagtcccagt cccacggcct cccgctgatg
      181 ccgcccggga aggtgagccg tgagtctccc tggaagagcg tcaccacgta gtcgtgcggc
      241 ccctcctcca gcaccgtcac ggggaggacg gccgagtagt cgtagccacc cgcgggccgc
      301 atcgggaaac cgcgatagaa ccccccaggt gagggtcgga tgaagagcac gaccgagtcg
      361 ggcggcgtca cttccgcgac gtggacccgg agctcggcct ctcgaccggc aaggaactcg
      421 ggcggcgtca acggcaggac cgatggcggc agcgagtcga ggggtggcgc gtgaaactcg
      481 tcgagaccga ggtggccgat ctgcccgggc agcgtctcga tgtccaccgg tccacccgcg
      541 ctcagcaggt agacgccggg agtgaccgtg aagcggccat tcgccgcggg ggcttccgac
      601 cgcctgaccg cccgaccgcc atcgttcatc gcttgaaccg tgaactggtt gccgagatcc
      661 ggcagccgca ccgtcatcgg ccaggcgcgg ctgatggcgc gcgtcacgat cttggcggga
      721 ttgggcggct cgaagggatc gcgcaccggc acggcgtcgg gatagacctc gagacgccac
      781 acgccggggc ggaccttgtc caggaaatag atcccctccc cctcgtacgt cacggtcgga
      841 gaggagccgt agccggcgat gcggcgcagg gcggccgggt cgggtggggt ggagctggtc
      901 gagcccgcgt acaggaatgc atcggccgcc accagctcgc ccaggttctc ggcgtaggag
      961 acgtggaagt cgccgaagcg cgtgttctgg ggataggcac cataggcgcg caggcgcggc
     1021 aggcggtgca tggcctccgc cgcgatgatc gcgctcatcg ccttgcgcgg cgtgtacgcc
     1081 aggctgaggt agtgggtctg ccatccgagg ttgcgcgagg cggtctgctg catgtcgtag
     1141 gtgaacatgg cggcgaactg tgtgcccacg gcacggaacg tccgcgccat cgccggatac
     1201 aggtagcccg tccgcagatc gggtgtgtcg aactcgtaca cgatgcgtgg cagccgggcg
     1261 agctcggggc gcagcatgtc gggataggcg tcgaccgcgc gtaggaagtt gccctgcagc
     1321 tcgtgcccgg agacgagacc ggagggatac cagccgaagg tcaccccctg cgccttggag
     1381 cgccggatcg cttcaccgat gcggaagtcc tggctcacgt tgtagaagac cagcttgctg
     1441 caccccgtgc tacgcaccgc ctccgtgagc gtgttgatgt agcggatcga gcccgcgaga
     1501 tcctcggcgt ggtgccacgg ctcgttgacc agctcgatga agatgatggc cggctcgtcc
     1561 ttgagcgcca ggtgcgtgta cgggttcacg tggttcagga tctggcgcaa atagttcacc
     1621 tgcgccgcga tggccgccga gtcggtcccc atgcgctccc tgccgaaccg ccggccgaac
     1681 ccgggatcgg tcgtatcctg tagcgcgtcg ggccagttcg ctccgtagag ctggatgggg
     1741 ctgaacagca tgtagatgcc gcgctcgcgt gcccgggcga tcagatagtc gagcaggtcg
     1801 aggtggtcgt tggcgatcag gttgccggcc gagtcggatg attcccagtc gccccagaag
     1861 gtgagccgca tgccgtccca acccatgcgg gcaaattgcg ccatgtcctc gtcgatcatc
     1921 cgcttgcgat cgccgtggac gtatccggcc gcccggtagt cggaggcggt gggcaggacg
     1981 tagttcgcac cgaagagcgt gagttcctgg cggttgtcgc gccagcggac tacgccctgg
     2041 ttgtcgaggt agacggcgcg gttggcgacc tgggcctgca accctgcgtc aatcgcgagc
     2101 gagaacaaga ggaccaggcg cgatttcgcc ccgatgtgcg tcaatcggat accggaggaa
     2161 tacatgaatc tcactatgct ggtgcccgcc agcggcacgg tgtcttgccg agctgcgtcg
     2221 tggtctccag ttacgtcaat tgtcgagcag gtgcgggtgt ggcgcaactg gtagacgcag
     2281 gattctcgca agatccgggg cgcaagccca tgt
//
LOCUS       DBNGZW010000713         1928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_678961, whole genome shotgun sequence.
ACCESSION   DBNGZW010000713 DBNGZW010000000
VERSION     DBNGZW010000713.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1928
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_678961"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1307
                     /locus_tag="AC16JC_16810"
     CDS             <1..1307
                     /locus_tag="AC16JC_16810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5166414.1"
                     /translation="HGPAAKERCIDLNEGVSILRNKREVFYDVIPWWPGIPFRGIDRW
                     WGCEISFDAVLDSQFTVKNIKRGAVPVRELKQAIKERIEPTRHAALERIRDHWKEAGA
                     LARADEQQLPLWRAKRDSQPVTVAAGQWRDPVVEPQRTQPRTRPLVFGLLLLLAFSAG
                     AYVAWPGGRGVGKEPSLPGEDVTTIAPPPAEVPRLRQLRVAHRAVVTVPQADTAATDS
                     APAPAAPPVEPVRVPAAGRTGFVVGSGQRGRVGEPATLPVVFEVRDSSGTALPGVAVS
                     LSIVNGRLVGPQPETDSLGQVRAQVVFGQRAGVATVVTGTVGALVREASLYPAAGTPA
                     KLVVVFDGDALASRIVVPAGGTAELRISCRDSYDNALSLVGLRATVGDDRVVQVTGVA
                     VDSLGGVVTVKAGRGGATNLVILGSGLRADLSAVVSPAAASP"
     gene            complement(1338..>1928)
                     /locus_tag="AC16JC_16815"
     CDS             complement(1338..>1928)
                     /locus_tag="AC16JC_16815"
                     /inference="COORDINATES: protein motif:HMM:NF019585.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR5166415.1"
                     /translation="MPAGSSLPITTVRRDETGERWHVFQAEPGSDALRDGMQVGAPCS
                     LQLDAKRRLALMRFHTALHVFNTVMLRGFDGWITGVRMDPARSHIDFRVNYVPKMVRR
                     IEDDVNDVLGRQLAIGSYTISAEEFQRRPDLLRTLEAQPPIEDGRVRVVEIAGFEAQA
                     CGGTHVRDTGAVGQIRIVKVDNKGKQNRRFYLELAE"
BASE COUNT          281 a          631 c          700 g          316 t
ORIGIN      
        1 gccacggtcc tgccgcgaaa gagcggtgca tcgatctgaa cgaaggggtg tcgattctgc
       61 ggaacaagcg tgaagtcttt tacgacgtca tcccgtggtg gccgggcatc ccgtttcggg
      121 gaatcgatcg ctggtggggc tgcgagatct cgtttgacgc cgtgctcgac agccagttca
      181 ccgtgaagaa catcaaacgt ggggccgtgc cggtccggga gctcaagcaa gcgatcaagg
      241 agcgcatcga gccgacgcgg catgccgcgt tggagcgaat cagggatcac tggaaggagg
      301 ccggggcgct ggcgcgggcc gacgaacagc agctgccgct gtggagagcg aagcgcgaca
      361 gccaaccggt cacggtcgcc gccggccagt ggcgtgaccc tgtcgttgag ccgcagcgga
      421 cgcaaccgcg caccaggccg ctggtgttcg gattgctgct gctgctcgct ttcagcgcgg
      481 gcgcttatgt cgcatggccg ggcggacgtg gtgtgggcaa ggagccgtcc ctgcctgggg
      541 aagacgtgac caccatcgcg ccgccgccgg cggaggtacc gcggttgagg cagctccgag
      601 tcgcccatcg ggcggtcgta accgtgccgc aggcggacac cgcagcgacg gattccgccc
      661 cggcaccagc agcgccgccg gtcgagcccg tgcgggttcc ggctgcgggg cgcaccggct
      721 tcgtggtggg gtcggggcag cgcgggcgcg tgggcgagcc cgccaccctc cccgtggtgt
      781 tcgaagtacg ggactcgtcg gggaccgcgc tgccgggcgt ggcggtgtcc ctctccatcg
      841 tcaacggacg gctggtcggc ccacagcccg agacggattc cctcggtcag gtgcgcgcgc
      901 aggtggtctt cggccagcgc gccggggttg cgaccgtggt caccgggacg gtcggcgcgc
      961 tggtgcgcga ggccagcctg tatcccgcgg ccgggacgcc tgcgaagctc gtggtcgtgt
     1021 tcgacggcga cgcgctcgcg agccggatcg tcgtgccggc cggtgggacg gccgagctgc
     1081 gcatatcgtg ccgggattcc tacgacaacg cgttgtcgct ggtgggactg cgcgccacgg
     1141 tgggcgatga tcgcgtggtt caggtgaccg gggtcgccgt ggactcgctg ggaggcgtgg
     1201 tgaccgtgaa ggcggggcgt ggcggggcga cgaacctcgt gatcctgggc tcgggactcc
     1261 gcgccgacct cagtgcggta gtatcccccg cggcagcgtc tccctgagga atgtgcgtct
     1321 tgtaacgctg gtacgcgtca ctcggccagc tccaaataga agcggcgatt ctgtttgccc
     1381 ttgttgtcga ccttcacgat cctgatttgc ccgacggccc cggtgtcgcg cacgtgcgtc
     1441 ccgccgcacg cctgcgcctc gaagcccgcg atctcgacca cccgcacccg gccgtcctcg
     1501 atgggcggct gggcctccag cgtccgcagc agatcggggc gtcgctggaa ctcctcggcg
     1561 ctgatcgtat acgagccgat agcgagctgc cgccccagca cgtcgttcac atcgtcttcg
     1621 atgcggcgca ccatcttcgg cacgtagttc acccggaagt cgatgtgcga gcgcgcagga
     1681 tccatccgta cgccggtgat ccacccgtcg aagccacgga gcatcacggt attgaagacg
     1741 tggagcgcgg tatgaaagcg catcaatgcg agacggcgct tggcatcgag ctgcagcgag
     1801 catggcgcgc cgacctgcat cccgtcgcgc agcgcgtcgc tgcccggttc cgcctggaag
     1861 acgtgccatc gctctcccgt ctcatcgcgg cgcaccgtcg tgatcggcag cgagctgccg
     1921 gcgggcat
//
LOCUS       DBNGZW010000714         2786 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_272143, whole genome shotgun sequence.
ACCESSION   DBNGZW010000714 DBNGZW010000000
VERSION     DBNGZW010000714.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2786)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2786)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2786
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_272143"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="AC16JC_16820"
     CDS             <1..1540
                     /locus_tag="AC16JC_16820"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR5166416.1"
                     /translation="QALDTERRTLAASEARWRALILNGEDAVLVVDPTGAIGYASPNA
                     DRVLGLPSGGLGDSVLSALPPDLRPAMLVLLEASQREPGRPRVQELQLGDGTPRYLEV
                     VATNLLADSAVNGIVLNLRDLTERRAMEEALRQSQEQLLHAQKMEALGRLAGGIAHDF
                     NNLMMAISGYTELVLQDPSISPTSRDDVAQIRRAVFRAAELTQQILAYSRRQMLQPVP
                     VNLNLLLGEASGMLRRLMPENIELRLALDPNLPDVLVDPGHMIQVVMNLALNARDAMP
                     NGGTLDIRTAAGARPSAEGNGGGPHAQLIVSDSGTGIDPKVVPLIFDPFFTTKPQGEG
                     SGLGLSTVYGIVKQSGGQIDVQSEINRGTTFTITLPPAPQVPARDVSGEVQGPPRSGG
                     EVILVVEDDDQVRTVVSKGLPRLGYEVLVTRNAEEALALVEKHPGRIDLLLTDVVMPG
                     LSGPQLADRLTARRPETRVVFMSGYPEAQDPALGFSLDGRSYLQKPFALSELAEKIRL
                     ALDE"
     gene            1676..>2786
                     /locus_tag="AC16JC_16825"
     CDS             1676..>2786
                     /locus_tag="AC16JC_16825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HIR5166417.1"
                     /translation="MPFLSRQSPYDVCIVGSGAGGGMAAHTLTQAGANVVLLEAGPLW
                     FSTEDAAMLQWAYRSPRRGASTKERQFGEFDAALGGFEIDGEPYTRAPGTKFDWFRSR
                     MLGGRTNHWGRISLRFGPSDFRRKSIDGLGDDWPIGYDDLKPFYDRVDRLIGLFGSRE
                     NLPNEPDGIFQPPPRPRCYELLVKQAADRLRITCIPSRLSIITKALGDRPACHYCGQC
                     YRGCATNSNFSSPAVLLKPALATGKLTIVPNAMAREVTTDARGLASGVAYIDKTTGQD
                     LHVRARVVVLAASALESARLLLNSTSTRFPEGLANSSGTVGRYITDTTGSDLGGFIPR
                     LADGIPHNEDGVGGMHLYMPWWLDNKTLDFPRGYHI"
BASE COUNT          435 a         1029 c          917 g          405 t
ORIGIN      
        1 ccaggcgctc gacacggagc ggcgcacgct ggcggcgagc gaagcgcgct ggcgcgccct
       61 gatcctgaac ggcgaggacg ccgtgctggt cgtcgatccg accggcgcga tcggctacgc
      121 ctcgcccaac gcggaccgcg tcctgggcct gccgagcggg gggctgggcg attcggtgtt
      181 gtccgccctg ccgccggacc tgcgtcccgc gatgctggtc ctgctggagg cgagccagcg
      241 cgagccggga cggccgcgcg tgcaggagct gcagctgggc gacggcacac cccgctacct
      301 cgaggtcgtg gccacgaacc tgctcgccga ttcggcggtg aacggtatcg tgctcaacct
      361 ccgcgacctc accgagcgac gggcgatgga agaggccctg cggcagagcc aggagcagct
      421 gctgcatgcg cagaagatgg aagcgctcgg ccggctcgcg ggcggtatcg cccacgactt
      481 caacaacctg atgatggcga tctcggggta taccgagctg gtgctgcagg acccgtcgat
      541 ctcccccacg tcgcgggacg acgtcgcgca aatccgccgc gccgtcttcc gcgcggccga
      601 gctcacgcag cagatcctcg cctacagccg gcgccagatg ctgcagcccg tcccggtgaa
      661 cctgaacctc ctgctgggcg aggcgagcgg catgctgcgg cgcctgatgc cggagaacat
      721 cgagctgcgg ctggcgctcg atcccaatct ccccgacgtg ctggtcgatc ccgggcacat
      781 gatccaggtg gtgatgaatc tcgcgctgaa cgcgcgggac gcgatgccca acggcggcac
      841 cctcgacatc cggaccgcgg ccggcgcgcg cccgtctgcg gaggggaacg gcggcggccc
      901 gcacgcccag ctcatcgttt ccgacagcgg tacgggcatc gatcccaagg tcgtcccgct
      961 catcttcgat ccctttttca ccaccaagcc gcagggcgag ggcagcggcc tcgggctgtc
     1021 caccgtgtac gggatcgtca agcagagtgg cggccagatc gacgtccaga gcgagatcaa
     1081 ccgcggcacc acattcacca tcaccctgcc ccccgcgccc caggtgccgg cgcgggacgt
     1141 gtcgggagag gtccagggcc cgccacgcag cggcggcgag gtgatcctcg tggtggagga
     1201 cgacgaccag gtccgcaccg tcgtctcgaa agggctgccg cgcctgggct acgaagtcct
     1261 ggtgacgcgc aacgccgagg aggcgctcgc gctggtggag aagcaccccg gccgcatcga
     1321 tctgctcctc accgacgtcg tgatgcccgg gctctccggc ccgcagctcg cggatcgcct
     1381 caccgcacgc cgtcccgaga cccgcgtcgt gttcatgtcg ggctacccgg aggcgcagga
     1441 ccccgccctg gggttctccc tcgacggccg cagctacctg cagaagccgt tcgccctctc
     1501 ggaactggcg gagaagatcc ggctggccct ggacgagtga gaggcggtcg tcggttttcg
     1561 gtcatcggtc atggggcgta gccgatctgc gtttcgcggc acatcattag gacgccgtca
     1621 aaagcgtcac caccgacaac cgagtaccga caaccgataa cgccttcgag atcccatgcc
     1681 atttctctca cgccagtctc cctacgacgt ctgcatcgtc ggctccggcg cgggcggcgg
     1741 tatggccgcc cacacgctca cgcaggccgg cgccaacgtc gtgctgctcg aagccggccc
     1801 gctctggttc tcgaccgagg acgcggcgat gctccaatgg gcctaccgct cgccccggcg
     1861 cggcgcatct accaaagagc ggcagttcgg cgagttcgac gcggcgctcg gcggcttcga
     1921 aatcgacggc gagccctaca cccgcgcgcc ggggaccaag ttcgactggt tccgcagccg
     1981 catgctcggc gggcggacca accactgggg ccgcatctcg ctgcgcttcg gtccctcgga
     2041 tttccggcgc aaatcgatcg acggcctggg ggacgactgg cccatcggct acgacgacct
     2101 gaagcccttc tacgaccggg tggaccgcct gatcgggctg ttcgggtcgc gcgagaacct
     2161 ccccaacgag cccgacggca tcttccagcc gccgccgcgg ccccgctgct acgagctcct
     2221 cgtgaagcag gccgccgacc ggctgcgcat cacctgcatc ccgtcgcgcc tgtccatcat
     2281 caccaaggcg ctgggcgacc ggcccgcctg ccactactgc gggcagtgct accgcgggtg
     2341 cgccaccaac tcgaacttct cctccccggc ggtgctgctc aaaccggcgc tggccaccgg
     2401 caagctcacc atcgtcccca acgcgatggc gcgcgaggtg acgaccgacg cgcgcggcct
     2461 cgccagcggc gtggcgtaca tcgacaagac caccggccag gacctgcacg tccgcgcccg
     2521 cgtggtcgtg ctggcggcga gcgcgctgga gtcggcgcgc ctgctgctca attccacctc
     2581 gacccggttc cccgagggcc tcgccaactc gagcggcacc gtcggccgct acatcaccga
     2641 tacgacgggc agcgacctgg gcgggttcat tccgcgcctc gccgacggca tcccgcacaa
     2701 cgaggacggc gtcggcggca tgcacctcta catgccgtgg tggctcgaca acaagacgct
     2761 cgatttcccg cgcggctatc acatcg
//
LOCUS       DBNGZW010000715         2909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_733148, whole genome shotgun sequence.
ACCESSION   DBNGZW010000715 DBNGZW010000000
VERSION     DBNGZW010000715.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_733148"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="AC16JC_16830"
     CDS             <1..332
                     /locus_tag="AC16JC_16830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012725849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HIR5166418.1"
                     /translation="TIGQNPVVYSGFEDAERVEVPDTGRLLPKEIAGFTRAGVYDEEH
                     EHTSFIQGGGHGGSHPHLVHEFVSAIRDRRNATVDAATAANWTMTGVCAHESAMRGGE
                     RIAIPQP"
     gene            332..1213
                     /locus_tag="AC16JC_16835"
     CDS             332..1213
                     /locus_tag="AC16JC_16835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019813383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR5166419.1"
                     /translation="MTVAPMQLGISTWVWTSPATTAVLETLLPHIAALGFDVIELPIE
                     EVGQFDIARVGELAREHGLGVSICAVIGPGRDLLLPDEAERGVTYLRSCIEVAQRLGS
                     PTVAGPFYSAVGRCWRSTTAERERDVDEIARVLRGLGAYAADHGVVLGVETLNRFETS
                     FLNTAAQALELIDRVGHPAVGVALDTFHLGIEEKHVGDALRAAGRRLVHLQVAENDRG
                     TPGTGSLPWNDIARALHEIEYSGRVVIETFSDRVEAIARAAAIWRPLAPDSDSLAREG
                     LAFLRSLLAPQPTVAVH"
     gene            1286..2836
                     /locus_tag="AC16JC_16840"
     CDS             1286..2836
                     /locus_tag="AC16JC_16840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5166420.1"
                     /translation="MAASVASTAPPAQLEGIHRDRLFVASCCALAVSAVAFAVIGDIL
                     GALKSQFILDNQQVGFIGGAAIWGFSVSIILLGPLCDVLGMKFLTRVAMVCHVLGVLI
                     MITATGFWTLFIGALVISLGNGLIEAVCNPLVVTLYPERKVHRLNQFHMWWPGGIVLG
                     GLASFALAQVGITSWKVRLGLILIPAVVYIALMLREQFPATENQASGVSTGEMFKTTL
                     TSPLFLLMLATMFITASIELGPGRWIPAVLASGGVPGILVLVWGSLLMAVLRRVASGP
                     LIKALSPTGILFSSMIVAAIGLYLLSNAQTAVSAFLAGTVFFIGVCFVWPTMLGFVSE
                     RVPRSGALGLALMGGAGMGVVGLVTSPWLGKVADETSHQRFIAEQPAVVATLDSASKA
                     LTAKLPSVPADQQADVKRAIDATNQVVASGQSGQLPPIATANAMRSIAATGIADPAVA
                     SVQALLGPAENFGGRTSFRRLVPFAAVAAVIFALLWINDRRKGGYRAQRIDEGVGAAP
                     RREPAGVH"
BASE COUNT          406 a         1004 c         1015 g          484 t
ORIGIN      
        1 agacgatcgg ccagaatccc gtggtgtact cggggttcga ggacgcggag cgcgtcgagg
       61 tgccggacac gggcaggctc ctgccgaagg agatcgctgg gttcacgcgc gccggcgtgt
      121 acgacgagga gcacgagcac acgtcgttca tccagggcgg cgggcacggc ggctcgcacc
      181 cgcatctcgt gcacgagttc gtgagtgcga tccgcgaccg gcgcaacgcc accgtggacg
      241 cggccacggc cgccaactgg acgatgaccg gtgtctgcgc ccacgaatcc gcgatgcgcg
      301 gcggcgagcg catcgccatc ccgcagccct gatgaccgtc gctccgatgc agctcggcat
      361 cagcacgtgg gtctggacgt cccccgcgac gacggcggtg ctcgaaaccc tgctgccgca
      421 catcgccgcg ctcggcttcg acgtgatcga gctgccgatc gaggaggtgg gccagttcga
      481 catcgcgcgc gtcggcgagc tggcgcgcga gcacggcctc ggcgtctcca tctgcgccgt
      541 catcggcccg gggcgcgatc tcctcctgcc ggacgaggcc gagcgcgggg tgacgtatct
      601 ccggtcgtgc atcgaggtgg cacaacggct cggctccccg accgtcgccg gtccgtttta
      661 ttcggcagtg ggacggtgct ggcgtagcac cacggccgag cgcgagcgcg acgtggacga
      721 gatcgcccgc gtgctgcgcg ggctcggcgc atacgcggcg gaccacggcg tggtgctcgg
      781 cgtcgagacg ctcaaccggt tcgagaccag cttcctgaac accgctgcgc aggcgctcga
      841 gctgatcgac cgggtcggcc accccgccgt cggcgtggcg ctggacacct tccatctcgg
      901 catcgaggag aaacacgtgg gcgatgccct ccgcgcggcg gggcggcggc tggtgcatct
      961 ccaggtggcc gagaacgacc gcgggacccc ggggaccggc tccctgccct ggaacgacat
     1021 cgcccgggcc ctgcacgaga tcgagtattc cggccgggtg gtgatcgaga ccttctcgga
     1081 ccgggtcgaa gccatcgcgc gggccgccgc catctggcgc ccgctcgcgc cggactcaga
     1141 ttcgctggcg cgcgaggggc tcgcgttcct gcggtccctc ctcgccccgc agcccactgt
     1201 agccgtacac taggaccgtt gctcgacccg cggaggggcc gaccacacgg ctcacccgtt
     1261 cgatctcaca ccgagaggaa gcccgatggc cgcatccgtc gcttcaaccg caccgcccgc
     1321 gcagctcgag gggatccacc gcgaccgact cttcgtcgcc tcgtgctgtg cgctcgccgt
     1381 gtccgccgtc gcattcgccg tgatcggtga cattctcggc gcactgaaat cgcagttcat
     1441 cctcgacaac cagcaggtcg ggttcatcgg cggggccgcc atctggggct tttcggtctc
     1501 gatcatcctc ctcggacccc tctgcgacgt gctggggatg aagttcctga cccgcgtcgc
     1561 gatggtgtgt cacgtgctcg gcgtgctgat catgatcacc gccaccgggt tctggacgct
     1621 gttcatcggg gcgctcgtga tctcgctcgg caacggattg atcgaagcgg tgtgcaatcc
     1681 actcgtcgtc acgttgtacc ccgagcggaa ggtgcaccgc ctgaaccagt tccacatgtg
     1741 gtggccgggt gggatcgtgc tcggcggcct tgcgagcttc gcgctcgcgc aggtcggcat
     1801 cacgtcgtgg aaggtccgcc tcggtctcat cctgatcccg gcggtggtgt acatcgccct
     1861 catgctgcgc gagcagttcc cggccacgga gaaccaggcg tcgggcgtct ccacgggcga
     1921 gatgttcaag acgacgctca cgtcgccgct cttcctgctg atgctggcga cgatgttcat
     1981 cacggcgagc atcgagctcg gaccgggccg ctggatcccg gcggtgctgg cgtcaggcgg
     2041 agttccggga atcctcgtcc tcgtgtgggg cagccttctc atggcggtcc tgcggcgcgt
     2101 cgcgtccggg ccgctcatca aggcgctgtc gccgacgggc attctcttct cgtcgatgat
     2161 cgtcgcggcg atcggcctgt acctgctcag caacgcacag acggcggtct ccgcgttcct
     2221 cgccggcacc gtgttcttca tcggggtgtg cttcgtctgg ccgacgatgc tcggcttcgt
     2281 ctcggagcgt gtgccgcgca gcggcgcgct cgggctcgcg ctgatgggcg gtgcgggcat
     2341 gggagtcgtg gggctcgtga cgtcgccctg gctgggcaag gtcgccgacg agacgtccca
     2401 ccagcggttc atcgcggagc agccggccgt ggtggccacg ctcgactcgg cgagcaaggc
     2461 gctgacggcg aagctgccct cggtccctgc ggaccagcag gccgacgtga agcgcgccat
     2521 cgacgcgacg aatcaggtcg tggcgtccgg acagagcggc cagcttccgc cgatcgcgac
     2581 cgcgaatgcg atgcggtcca tcgccgccac ggggatcgcc gatccggcgg tcgccagcgt
     2641 acaagcgttg ctcggcccgg cggagaactt cgggggccga acgtcattcc gtcgcctcgt
     2701 cccattcgcc gcggtcgctg cggtgatctt cgcgctgctc tggatcaacg atcgtcgcaa
     2761 gggcggatac agagcccaac ggatcgacga gggggtgggc gcggcgccgc ggcgagagcc
     2821 ggcgggggtg cactagcggc gcgcttcgcg cacgttcacg gtaatcggta gacggtaatc
     2881 ggccggcctc gggaagcacg ttccccccc
//
LOCUS       DBNGZW010000716         4348 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_885119, whole genome shotgun sequence.
ACCESSION   DBNGZW010000716 DBNGZW010000000
VERSION     DBNGZW010000716.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4348)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4348)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4348
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_885119"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /locus_tag="AC16JC_16845"
     CDS             complement(<1..822)
                     /locus_tag="AC16JC_16845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HIR5166421.1"
                     /translation="MQPVATAPGAPIRPAVQVTAQDEDGETLTDFTAAVTLSISEGTG
                     DEDATLSGTLTVSAVAGVASFTDLRIDKAEKGYRLTATAAGFAGATSAPFDIAGGAVA
                     ETPAAAAPAAAAPEAAPPADTAEEAQPTLRATPFDGALTLDGRLDEPAWRTADSITNL
                     TMIEPEEGGVPAGQTIVKVLATPGELVFGVMCRDSNPAGIVAFAKTRDFDLELEDHVL
                     IVLDPFQDGRSGYVFAVNPNGARFDGLVSAQGEEVNTDWDAIWEAATSRDATGWTL"
     gene            1211..2941
                     /locus_tag="AC16JC_16850"
     CDS             1211..2941
                     /locus_tag="AC16JC_16850"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF051540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5166422.1"
                     /translation="MAEAQLTRRLVSADYSPRIRAERLIASSRLVLAAFSLIAVWLDP
                     YRPATHEPTLYFLLLGYVVFAAVIATIVWLAHVPLARLGFVTHVLDLSVFSLLTYLTE
                     GPTSPFVTYFTFSLVAATLRWQWRGVLWTAAAALIAFNGAGIYASEVLNDPAFEENRF
                     VVRSVYLAVMAGLLAYLGAYEERRRRELSELAAWPATRVRGPELPEPEVLESTARILG
                     ARQVVLAWEEADEPWLHLASWCEGEFHAWREAPGTFQPVVAEQLAALTFLSKDVRAAM
                     PIVLHRDHQVPQAWRGTPIHPALQARFDMTSVLCLPLRGECFEGHLLALDKPHMTVDD
                     LLLGEVVAHEVASSMDQSLLSRRLRQAAAAEERMRLSRDLHDGVLQSLTGAALQLETV
                     QRLWETEPRAARERLVAIQRLIIEEQRDLRLSVHASKVAPIWLAPGFGGLDTGLRQLI
                     HRLESVWGLRVDLRLEHLDDRSSDLLASDICFIVQEALVNAARHAAASQVCVAVSREN
                     GRVHVAVGDNGRGFPFEGDYDHAKLDALQLGPVMLKERVKSLGGSLAIHSSPAGARLD
                     ILLPRGRERR"
     gene            2943..3581
                     /locus_tag="AC16JC_16855"
     CDS             2943..3581
                     /locus_tag="AC16JC_16855"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5166423.1"
                     /translation="MPIRLVLADDHPIVLDGLENLFRLEPDFRVVARCVNGEECLAAV
                     RRYEPDVLLLDIRMPRHDGLTVLRELSREEHTTKVVLLAAALEEEEVLEALRLGVRGM
                     LLKELAPQMVVQCVRKVHAGGQWVEKQAFGRALDTLLRREAGEREAASVLTPREIEMV
                     SMVARGLRNKEMSQRLSISEGTVKIHLHNIYRKLKVENRVELILYAQAKRLV"
     gene            3638..>4348
                     /locus_tag="AC16JC_16860"
     CDS             3638..>4348
                     /locus_tag="AC16JC_16860"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF013335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HIR5166424.1"
                     /translation="MTDPAPSSTRMPLPLVIDTDPGIDDTLALLLALRSPELDVRGIA
                     VSYGNTTVEQAFRNCVEILRRAGRRLTLGVSARRPLTRALAVALETHGATGLGDAPVP
                     PAGVILDFVKPLDRLLADQPEAVTLVTLGPVTGLALALRRDAALVRRTVKRHVAMIGN
                     LRAAGNTTRFSEFNAWCDPEALAEVLRAELPTELVGLDVTRKFLLAGHEIDRLGRSED
                     ALERWLHAALRFYLDFHRR"
BASE COUNT          626 a         1495 c         1523 g          704 t
ORIGIN      
        1 gagggtccag ccggtcgcgt cgcgcgaggt cgccgcttcc cagatcgcgt cccaatcggt
       61 gttgacctcc tcgccctgag cggaaaccag accgtcgaag cgggcgccgt tcggattcac
      121 cgcgaacacg tagcccgagc ggccgtcctg gaacggatcg aggacgatca gcacgtggtc
      181 ttccagctcc aggtcgaagt cgcgcgtctt ggcgaacgcg acgatgccgg cggggttcga
      241 atcgcgacac atcacgccga agacgagctc gcccggagtc gccaggacct tcacgatcgt
      301 ctggccggcg ggaacgccgc cttcttcggg ctcgatcatc gtgagattcg tgatcgagtc
      361 cgccgtacgc cacgccggct cgtcgagccg gccgtcgagg gtgagggcac cgtcgaacgg
      421 ggtcgccctc agcgtgggct gcgcctcctc agcggtgtcc gccggaggcg cggcctcagg
      481 cgcggcggcc gcgggagccg cggccgctgg agtctctgcg accgcgccgc ccgcgatgtc
      541 gaagggggca ctcgtcgccc cggcaaagcc cgcggcggtc gcggtgagcc ggtagccttt
      601 ctccgccttg tcgatgcgca ggtcggtgaa gctggccacg cccgccactg cggataccgt
      661 caaggtgccc gagagggtcg cgtcctcgtc tcccgtaccc tcgctgatcg acagcgtcac
      721 tgcggcggtg aagtcggtga gggtctcccc gtcctcgtcc tgcgccgtca cctggacggc
      781 gggacggatg ggcgctccgg gtgcggtggc gactggctgc acggtaaaga cgagttttgc
      841 cgagtctcga ggtcccgtac tgcccggcag gagcagcgaa acggcgaggc aagaacacca
      901 ggcgatcatg tgtcgtcctt accaggggtg cgtcgtgggc aaggcggtgg cgttccgcgc
      961 tcctgtgcaa ttcccaggcg cgcatcttgc caccaattgg ctgcgtcccg gattgacggg
     1021 gaccaacggt ggagtcaggg gcggcgggtc accatagatg gccggataca ggcacgtata
     1081 actttgggta gtcatccgct attgccgacc ccgagcatcg gttcgcggcg gcgacgcaac
     1141 agcccggacg gctatccacc gtttcccccg cgggctatta ttgccggcca ccggcgccgc
     1201 aggcaccgca atggctgaag cgcagttgac caggcggctc gtgagcgcgg actattcccc
     1261 ccggatccgc gccgagcgtt tgattgcgtc cagccgcctg gtgctggctg cgttttccct
     1321 catcgctgtc tggctggacc cgtaccggcc ggccacccac gagccgacgc tgtacttcct
     1381 gttgctcggc tacgtggtct tcgcggcggt gatcgcgacg atcgtctggc tggctcacgt
     1441 ccccctcgcc agactgggat tcgtgacgca tgtgctggac ctctcggtgt tctcgctcct
     1501 gacctacctg accgagggac ccaccagccc gttcgtcacc tacttcacct tctccctcgt
     1561 agccgccacg ctgcggtggc agtggcgcgg tgtgctctgg accgccgccg ccgcgctcat
     1621 cgcgttcaac ggggcgggca tctatgcgag cgaggtcctg aacgacccgg cattcgagga
     1681 gaaccggttc gtcgtccgca gcgtgtacct ggcggtgatg gccggcctgc tcgcctacct
     1741 cggggcctac gaggagcggc ggcggcggga gctgtccgag ctggcggcct ggcccgccac
     1801 gcgggtccgc ggacccgaac tgccggagcc ggaggtgctc gaaagcaccg cgcggatcct
     1861 cggcgcccga caggtggtgc tcgcgtggga ggaggcggac gagccatggc tgcacctcgc
     1921 ctcgtggtgc gaaggagagt tccacgcctg gcgcgaagcc ccggggacct tccaaccggt
     1981 ggtcgcggag cagcttgccg cactgacctt cctttcgaag gacgtccgcg cggcgatgcc
     2041 gatcgtcctg caccgcgacc atcaggtccc gcaggcgtgg cgcggcacgc cgatccatcc
     2101 ggcgctgcag gcgcgattcg acatgacctc ggtgctctgc ctgccgctgc gcggcgaatg
     2161 cttcgaggga cacctgctgg cgctggacaa gccgcatatg acggtggacg atctgctgct
     2221 cggtgaggtc gtcgcgcacg aggtggcctc ctccatggat cagtccctcc tgtcgcggcg
     2281 gctccgtcag gcggccgccg cggaggagcg gatgcgactc tcgcgcgatc tgcacgacgg
     2341 ggtgctgcag tcgctcaccg gcgccgcgct gcagctcgag acggtgcagc gtctctggga
     2401 gacggagccg cgcgccgcgc gggagcgact ggtggcgatt cagcggctga tcatcgaaga
     2461 gcagcgggac ctccggctct cggtccacgc atcgaaggtg gccccgatct ggctggcgcc
     2521 gggattcggg ggcctcgaca ccggcctgcg ccagctgatc caccggctgg agagtgtgtg
     2581 gggactgcgc gtggacctgc ggctggagca cctcgacgac cgctcgtcgg acctcctcgc
     2641 gtccgacatc tgcttcatcg ttcaggaggc gctcgtcaat gccgcccgcc acgccgcggc
     2701 ttcgcaggtc tgcgtggcgg tcagccggga gaacgggcgg gtccatgtcg ccgtcggcga
     2761 caacggccgc ggtttcccgt tcgagggcga ctacgatcat gcgaaactgg atgcactgca
     2821 gctcggcccg gtgatgctca aggaacgggt caagtcgctg ggcgggtcgc tcgccatcca
     2881 ctcctcgccc gccggagccc ggctcgacat tctcctgccg cgcggccggg aaagacgcta
     2941 gcgtgcccat ccgcctcgtg ctcgccgacg atcacccgat cgtgctcgac ggactggaga
     3001 acctgttccg cctcgaaccc gacttccgcg tggtggcgcg ctgcgtgaac ggcgaagagt
     3061 gcctcgccgc ggtgcgccgc tacgagcccg atgtgctgct gctcgatatc cgcatgcccc
     3121 ggcacgacgg cctgaccgtg ctgcgggagc tgagtcgcga ggagcacacc accaaggtcg
     3181 tgctcctcgc ggccgcactg gaagaggaag aggtgctgga ggcgctccgc ctcggcgtgc
     3241 gcggcatgct gctcaaggag ctcgcgcccc agatggtcgt gcagtgcgtg cgcaaggtcc
     3301 acgccggcgg ccagtgggtc gagaagcagg cgttcggccg ggcgctcgac acgttgctgc
     3361 gacgcgaggc cggcgagcgc gaagcggcca gcgtgctgac gccgcgcgag atcgaaatgg
     3421 tcagcatggt ggcgcgcggg ctgcgcaaca aggagatgtc ccagcggctg tccatcagcg
     3481 aggggacggt gaagatccac ctgcacaaca tctaccggaa actgaaggtc gagaaccgcg
     3541 tcgagctgat cctctacgcg caagccaagc gtctcgtctg agcgatgtcc ggcgcccgac
     3601 tattgctaag gcgagagggg acccgtaggc ttgtgccgtg accgacccgg cgccctcgag
     3661 tacccgcatg ccgctgcccc tcgtcatcga caccgatccc ggcatcgacg acacgctcgc
     3721 gctgctcctc gcgctgcgct cgcccgagct ggacgtgcgc ggcatcgcgg tcagctacgg
     3781 caataccacg gtcgagcagg cgttccgcaa ctgcgtcgag atcctgcggc gcgcggggcg
     3841 gcgcctcacc ctgggggtga gtgcccggcg gccgctgacg cgcgccctcg cggtggcgct
     3901 cgaaacccat ggtgccaccg gtctgggtga cgccccggtt ccgccggccg gcgtcatcct
     3961 cgatttcgtg aaaccgctcg accgcctcct ggccgaccag ccggaggcgg tcaccctggt
     4021 gacgctgggt ccggtgacgg gcctcgcgct ggcgctgcgc cgcgatgcgg ccctggtgcg
     4081 gcgcacggtc aaacgccacg tcgcgatgat cggcaatctg cgcgctgccg ggaacacgac
     4141 gcggttctcc gagttcaacg cctggtgcga tccggaggcg cttgcggaag tgctgcgcgc
     4201 cgagttgccg accgagctcg tgggcctcga cgtgacgcgg aagttcctgc tggcggggca
     4261 cgagatcgac cggctgggac ggagtgagga cgccctggag cgctggctcc acgccgcgct
     4321 ccgcttctac ctggatttcc accgccgg
//
LOCUS       DBNGZW010000717         2770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1072726, whole genome shotgun sequence.
ACCESSION   DBNGZW010000717 DBNGZW010000000
VERSION     DBNGZW010000717.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2770
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1072726"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..974)
                     /gene="rplB"
                     /locus_tag="AC16JC_16865"
     CDS             complement(135..974)
                     /gene="rplB"
                     /locus_tag="AC16JC_16865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HIR5166425.1"
                     /translation="MGIRQFKPVTAATRFRSVSDFAEITSATPERTLLEPLRRTGGRN
                     NKGHVTTRHQGGGHKRQYRRIDFRRNKFGIPATVTTVEYDPNRTCRIALVTYADGEKR
                     YILHPKGLVVGDTVVSGPGSDIRTGNALPLFEVPLGTTVHNVELKIGRGGQMCRSAGT
                     GAQVVAKEGNYVTLRLRSTEMRRVRGECLATIGEVGNAEHELLSVGKAGKTRWLGRRP
                     RVRGVAMNPVDHPLGGGEGKSSGGRPPVSPWGKVEGRKTRHKKKASTKLIVRGRKRGK
                     ATQ"
     gene            complement(976..1263)
                     /locus_tag="AC16JC_16870"
     CDS             complement(976..1263)
                     /locus_tag="AC16JC_16870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HIR5166426.1"
                     /translation="MPDLHRLIVGPVVTEKSSAAYQARKEYAFRVHPEATKPQIREAI
                     EKLFSVSVTDVRTLVVRAKQRSYGRHVGRRPSWKKAIVKLKDGDTIPVFEG"
     gene            complement(1256..1900)
                     /gene="rplD"
                     /locus_tag="AC16JC_16875"
     CDS             complement(1256..1900)
                     /gene="rplD"
                     /locus_tag="AC16JC_16875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HIR5166427.1"
                     /translation="MIEAPHFSAAGAKKKAIALPEAIFDGRVNEDVLHRAVVTYLANQ
                     RQGTAATKTRSFVSGGNQKPWRQKGTGRARQGSTRAPHWRHGGVVFGPHPRDYRMSIP
                     KKVRQLARKSALNARAREGALLVVDALAYEKPKTKPFAELLDKLGLAGRKVLVLTADS
                     PTSHNVYLAGRNIPDVRIQRFVDATAYDVLWAKTVVVEAPALSGAEPEEASTDA"
     gene            complement(1893..2513)
                     /gene="rplC"
                     /locus_tag="AC16JC_16880"
     CDS             complement(1893..2513)
                     /gene="rplC"
                     /locus_tag="AC16JC_16880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013259729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HIR5166428.1"
                     /translation="MNALIGRKLGMTRLFDEEGAHVPVTVIEAGPCPVIQVAGDRVQL
                     GFGAKKAKRTTKALAGHVKAAGLETGPRRLKSFPLTGEAPKAGDLVTVAIFAPGERVK
                     VSGVTKGKGFQGVVHRYHFGGGPATHGNTRHRKPGSIAPGTDPSRIIKGKRMPGHMGA
                     RHFTELGLRVVRIDAERNLLFVRGAVPGPMNGYVTVRKQGGRSRHD"
     gene            complement(2590..>2770)
                     /gene="rpsJ"
                     /locus_tag="AC16JC_16885"
     CDS             complement(2590..>2770)
                     /gene="rpsJ"
                     /locus_tag="AC16JC_16885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014546117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HIR5166429.1"
                     /translation="KRQMWTVLRSPHIDKKSREQFELKTHKRLIDIVDSRSQTVDALT
                     KLDLPAGVDVEIKVG"
BASE COUNT          372 a         1037 c          869 g          492 t
ORIGIN      
        1 tcaggagcga cggctccacg tacggaccct tcttgacgct gcgtgccatt tcactctcca
       61 ttgcggattg gcgattgcgg attgcggatt gaacaccgcc gacaaatccg caatccacaa
      121 tccgaaaacc gcaattactg ggtggccttc ccccgtttac ggccgcggac aatcagtttc
      181 gtcgaggcct tcttcttgtg acgcgtcttg cgcccttcca ccttgcccca cggcgacacc
      241 ggcgggcgcc cgcccgagga cttgccctcg ccgccgccca gcgggtgatc caccgggttc
      301 atcgccacgc cgcgcacccg gggccggcgg cccagccagc gcgtcttgcc cgccttgccc
      361 accgagagca gctcgtgctc ggcattgccg acttcgccga tggtggcgag gcactcgccc
      421 cgcacgcgcc gcatctcggt cgagcgcagc cgcagggtga cgtagttgcc ttccttcgcc
      481 acgacctgcg cgcccgtgcc cgccgagcgg cacatctggc cgccgcgccc gatcttgagc
      541 tccacgttgt gtaccgtggt gccgagcggg acctcgaaca gcggcagcgc gttgccggtg
      601 cggatgtccg agcccggccc cgacaccacc gtgtctccca ccaccaggcc cttgggatgc
      661 aggatgtagc gcttctcccc gtcggcgtag gtcacgagcg cgatgcggca ggtccggttc
      721 gggtcgtact cgaccgtcgt caccgtggcc ggaatgccga acttgttccg ccggaaatcg
      781 atccggcggt actggcgctt gtggccgccg ccctggtggc gcgtcgtgac gtgccccttg
      841 ttgttgcgcc cgcccgtgcg ccgcagcggc tcgagcagcg tccgctccgg cgtggcgctc
      901 gtgatctcgg cgaaatcgct caccgagcgg aaccgcgtgg ccgcggtgac cggcttgaac
      961 tgtcggatgc ccatatcagc cctcgaacac cgggatcgtg tcgccgtcct tcagcttcac
     1021 gatcgccttc ttccaggacg ggcgccggcc gacgtgccgc ccgtagctgc gctgcttggc
     1081 gcgtaccacg agggtccgga cgtcggtgac gctcacgctg aacagcttct cgatcgcctc
     1141 gcgaatctgc ggcttggtcg cctcgggatg cacgcggaac gcgtattcct tgcgcgcctg
     1201 gtaggcggcc gacgacttct cggtcaccac cggtcccacg atcagccggt gcaggtcagg
     1261 catcggtgct cgcctcctcg ggctccgcac ccgacagcgc cggcgcctcg acgaccaccg
     1321 tcttggccca aagcacgtcg tacgcggtcg cgtccacgaa gcgctggatg cgcacgtccg
     1381 ggatattgcg gcccgcgagg tacacgttgt gcgacgtcgg cgaatccgcc gtcagcacca
     1441 gcaccttgcg cccggccagg cccagcttgt ccagcagctc ggcgaacggc ttggtcttcg
     1501 gcttctcgta ggccagcgcg tccaccacca gcagggcgcc ctcgcgcgcc cgcgcgttca
     1561 gcgccgactt gcgcgccagc tggcgcacct tcttgggaat cgacatgcgg tagtcgcgtg
     1621 gatgcggtcc gaacacgacc ccgccgtgcc gccagtgcgg cgcgcgcgtc gagccctgcc
     1681 gcgcccgccc ggtgcccttc tgccgccacg gcttctggtt gccgcccgag acgaagctgc
     1741 gcgtcttggt cgcggcggtt ccctgccgct ggttggcgag ataggtgacc accgcgcggt
     1801 gcagcacgtc ctcgttcacg cggccgtcga agatcgcctc cggcagcgcg atcgccttct
     1861 tcttcgcccc ggcggcggaa aagtgcggcg cctcaatcat ggcggctcct gcctccctgc
     1921 ttgcgtaccg tcacgtagcc gttcatcggc ccgggcaccg cgccgcgcac gaacaacagg
     1981 ttccgctcgg cgtcgatgcg caccacccgc aggcccagct cggtgaagtg acgcgcgccc
     2041 atgtggcccg gcatccgctt ccccttgatg atgcgggacg gatcggtgcc cggggcgatg
     2101 gagcccggct tgcggtgccg tgtgttgccg tgggtcgccg gcccgccgcc gaagtggtag
     2161 cggtgcacga cgccctggaa ccccttgccc ttggtcaccc cggacacctt cacccgctcg
     2221 cccggcgcga agatcgcgac cgtcacgagg tcacccgcct tcggcgcctc gcctgtgagg
     2281 gggaaactct tcaggcgccg cggtcccgtt tcgagcccgg cggccttcac gtgccccgcc
     2341 agcgccttgg tcgtgcgctt ggccttcttc gccccgaagc ccagctgcac gcggtctccg
     2401 gcgacctgga tcaccgggca gggcccggct tcgatcacgg tcaccggcac gtgcgcgccc
     2461 tcttcgtcga acaggcgcgt catgccgagc ttgcggccaa tgagtgcgtt cattttgcga
     2521 accccgaata ccgacgaccg acgaccgacg acgtcgtcgg tacacggtca tcggtcgtca
     2581 aatgcgtgct catccgacct tgatctccac atccacaccc gccggcagat ccagcttcgt
     2641 caacgcatcc accgtctgcg agcgcgagtc cacgatgtcg atcagccgct tgtgcgtctt
     2701 cagctcgaac tgctcccggc tcttcttgtc gatgtgcggg ctgcgcagca ccgtccacat
     2761 ctggcgcttg
//
LOCUS       DBNGZW010000718         3009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_544046, whole genome shotgun sequence.
ACCESSION   DBNGZW010000718 DBNGZW010000000
VERSION     DBNGZW010000718.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3009
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_544046"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="AC16JC_16890"
     CDS             complement(<1..403)
                     /locus_tag="AC16JC_16890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HIR5166430.1"
                     /translation="MAPLHRFVVGTAGHIDHGKTALVKALTGVDTDRWEEEKRRGITI
                     DLGFAPIDLGEGLEASVVDVPGHEGFVRNMLAGATGIDAALLVVAADEGIMPQTAEHL
                     AIVELLGVRRGIPVLTKRDLVDDDWLALVETE"
     gene            complement(379..1176)
                     /gene="recO"
                     /locus_tag="AC16JC_16895"
     CDS             complement(379..1176)
                     /gene="recO"
                     /locus_tag="AC16JC_16895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR5166431.1"
                     /translation="MSLVTTPAVVLRAYRYSETSKIVRLATRDLGVQSAIAKGVSRPK
                     SRFGAGLELLSEGEAQLYYRDNRELQTLAAFDVERLRRELAGDLDRFAAATVLAELMV
                     RMASPAPLPALYDAFTGALDDLVVVSGDTLHAAGIRAVWRLLGVLGFEPSLRACVRDS
                     AAVGEGGEGVPVAFSTAEGGVLCETCLQSRPPATLTRLPWEAWRDLCAMLDPRAPLPQ
                     LDAPHAAAHRRLAARFVRHHLEDAGTAESLPALVFWERRAWPPSIAS"
     gene            complement(1201..>3009)
                     /locus_tag="AC16JC_16900"
     CDS             complement(1201..>3009)
                     /locus_tag="AC16JC_16900"
                     /inference="COORDINATES: protein motif:HMM:NF024371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing transglutaminase
                     family protein"
                     /protein_id="HIR5166432.1"
                     /translation="DDPYIYLLDDGVVVVQADGRTTRTYRQVVQILNQDAVEDWAEQS
                     FGYDAGREKLRINWVRVVKPDGTVISDGPSHRQESSSPVAMSAPVYSDSKVIRITLAG
                     VAPGTIVDYSFTTERFKPPLAGDFYSGWSVHTARLTRRSRLLLDVPVGMNPHIQERNL
                     HFRPQVVEARGRRVYQWATRDVPRVEREPYAGSPDTVYAGIDVAGIDSWADIARWYAG
                     LTSDRYAVGPALERKLTELIAQARTLEDSLKAVHRWVAQDIRYVSLSLGVGGYQPRVP
                     GDVLETGYGDCKDKATLFVALLGKMGVTAFPVLLSSSGTADSTLPTLGQFDHMIAAIP
                     VPGGYRFVDLTASLAPFGTLPPGEQGSFAVVVHPDGRGETVTLPLDPVSDNLDVSTIQ
                     GELTPEGMFNGHVGRRVSGAPQFSMRNDFSSPIDSTQRTRIARTIANAMFPGGSGDSL
                     VLFDGKDLAAEPQMAVAIHNGRAATSSGNTVILTLPIRTYATQGLADDLRARGPRRFP
                     ISAPGVSGEGVDEEDLSLTLPEGWHARLPANVDAPSDFGRYEARYTQDGRVLHVTRRL
                     TGARGVYSPDRVGDLIAWFETVARDDARYIVIERGP"
BASE COUNT          450 a         1089 c         1036 g          434 t
ORIGIN      
        1 gctcggtctc gaccagcgcc agccaatcgt cgtccaccag gtcgcgcttg gtgagcaccg
       61 ggatgccgcg ccgcactccc agcagctcca cgatcgcgag gtgctccgcg gtctgcggca
      121 tgatcccctc gtcggccgcc acgaccagta gcgccgcatc gatcccggtc gcgccggcga
      181 gcatgttccg gacgaagcct tcgtgacccg ggacgtccac cacgctcgcc tccaggccct
      241 cgccgaggtc gatgggggcg aaaccgaggt cgatcgtgat gccgcgccgc ttctcctctt
      301 cccagcggtc ggtgtccact cccgtcagcg ccttgacgag cgcggtcttg ccgtggtcga
      361 tgtgaccggc ggtgccgact acgaagcgat ggaggggggc cacgcgcggc gctcccagaa
      421 gacgagcgcg gggaggctct ccgccgttcc cgcgtcctcg aggtgatggc ggacgaagcg
      481 ggcggcgagg cgccggtgcg cggcggcgtg cggcgcgtcg agctgcggca gcggggcgcg
      541 tgggtcgagc atggcgcaga gatcccgcca cgcctcccac ggcagcctgg tgagggtcgc
      601 ggggggccgc gactgcaggc acgtctcgca caacacacca ccctcggcgg tgctgaacgc
      661 caccggcaca ccctccccgc cttcgcccac cgccgcggaa tcccgcacgc aggcgcggag
      721 cgacggctcg aatccgagca ccccgagcag gcgccagacg gcgcgaatcc ccgcggcatg
      781 cagcgtgtcc ccggagacca ccaccaggtc gtccagtgct ccggtgaacg catcgtacag
      841 cgcggggagc ggcgccgggg acgccatgcg caccatcaac tcggccagca ccgtggcggc
      901 ggcgaagcga tccagatccc cggccagctc gcggcgcagc cgctccacgt cgaacgccgc
      961 cagggtctgc aattcacggt tgtcacggta gtagagctgt gcttcaccct cgctcagcag
     1021 ctcgaggccg gcaccaaagc ggctcttggg acgcgacacg cccttggcga tggcgctctg
     1081 caccccgagg tcccgcgtcg cgagacggac gattttcgac gtctcgctgt agcgataggc
     1141 acgcagcacg acggcgggag tcgtgaccag ggacatgccc tacaagatag ccacccccgg
     1201 tcagggtccc cgctcgatca cgatgtatcg cgcgtcgtcc cgggcgacgg tttcgaacca
     1261 ggcgatgagg tcccccaccc ggtcgggcga gtagacgccg cgggctccgg tcaggcgacg
     1321 cgtcacgtgc agcacccgtc cgtcctgcgt gtagcgcgcc tcgtagcgcc cgaaatcgct
     1381 gggcgcgtcc acgttcgccg gaagccgcgc atgccacccc tcgggcaggg tcaggctcag
     1441 gtcttcctcg tccaccccct ccccgctgac gcccggagcg ctgatgggaa agcgtcgggg
     1501 gccgcgcgcc cgcagatcgt cggcgagccc ctgggtggcg tacgtgcgga tcggcagcgt
     1561 caggatcacg gtgttgcccg agctcgtggc cgccctcccg ttgtggatgg cgacggccat
     1621 ctgcggctcg gcggcgaggt ccttgccgtc gaacagcacc aggctgtccc cgctcccgcc
     1681 cggaaacatc gcgttcgcga tggtgcgcgc gatccgggtt cgctgcgtgg aatcgatcgg
     1741 cgagctgaag tcgttgcgca tggaaaactg gggcgcgccg gacacgcgcc gccccacgtg
     1801 accgttgaac atcccctccg gcgtcagctc gccctggatg gtcgagacgt cgaggttgtc
     1861 gctcaccgga tccagcggca gcgtgaccgt ctcgccgcgc ccgtcgggat ggaccaccac
     1921 cgcgaagctc ccctgttcac cgggcggcag ggtcccgaac ggcgcgaggc tcgccgtgag
     1981 atccacgaag cggtagcccc cgggcacggg aatcgccgcg atcatgtggt cgaactggcc
     2041 cagcgtcggc agggtgctgt ccgcggtccc gctcgagctg aggagcaccg ggaaggccgt
     2101 cacgcccatc ttgccgagca acgccacgaa cagggtcgcc ttgtccttgc agtcgccata
     2161 gccggtttcg agcacgtccc ccgggacgcg cggctggtag ccgccgaccc cgagcgacag
     2221 cgagacgtac cggatgtcct gggccaccca gcggtgcacg gccttcagcg aatcctcgag
     2281 cgtgcgagcc tgtgcgatca actccgtcag cttgcgctcg agcgcggggc cgacggcgta
     2341 gcggtcgctg gtgagcccgg cgtaccagcg ggcgatgtcg gcccaactgt cgatgcccgc
     2401 cacgtcgatg ccggcgtaca ccgtgtcggg cgacccggcg tacggctcac gctcgacgcg
     2461 cggcacgtcc cgcgtggccc actggtacac gcggcggccg cgcgcctcga ccacctgcgg
     2521 ccggaaatgg aggttgcgct cctggatgtg cggattcatc cccaccggga cgtcgagcag
     2581 caggcgcgag cgccgcgtga gacgcgccgt atgcaccgac caccccgagt agaagtcccc
     2641 agcgaggggc ggcttgaacc gctcggtcgt gaagctgtag tccacgatgg tgcccggcgc
     2701 cacgccggcc agcgttatcc ggatgacctt gctgtcggag tagaccggcg ccgacatggc
     2761 gaccggagag gaggactcct ggcggtggct cgggccgtcg ctgatcacgg tgccatccgg
     2821 cttcaccacg cgcacccagt tgatgcgcag cttctcgcgg cccgcgtcgt agccgaacga
     2881 ctgctcggcc cagtcttcca cggcatcctg gttcaggatc tggacgacct ggcggtaggt
     2941 ccgcgtggtg cggccgtccg cctggaccac cacgaccccg tcgtccagca gatagatgta
     3001 ggggtcgtc
//
LOCUS       DBNGZW010000719         7213 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_679624, whole genome shotgun sequence.
ACCESSION   DBNGZW010000719 DBNGZW010000000
VERSION     DBNGZW010000719.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7213)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7213)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7213
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_679624"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..794
                     /locus_tag="AC16JC_16905"
     CDS             <1..794
                     /locus_tag="AC16JC_16905"
                     /inference="COORDINATES: protein motif:HMM:NF025702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HIR5166433.1"
                     /translation="GCTKDATFVEPIPPLAAIHFVHAVPDTMPMDFRVVDIVSNAGLF
                     DASFRTANIFYTGIEAGSRQIRVFISSTNPAVTGVPIFDTTFAFTADADYTFIHAGFA
                     RTGNPPARTVWIVPDNATAPAAGQVGLRLIHAGAGLGALDVNVTRHAADTLPDAPLVG
                     GLTYSTVGSYLAMGRDSVAADSLRLVVTAAGTKAPVLFSLKLPAGAAGTSSADPIAGA
                     RVAGSVMTAVLVPPSVVGSAAPQGGAFAAPSAVILVDRRPPKTTP"
     gene            complement(885..1769)
                     /locus_tag="AC16JC_16910"
     CDS             complement(885..1769)
                     /locus_tag="AC16JC_16910"
                     /inference="COORDINATES: protein motif:HMM:NF013123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HIR5166434.1"
                     /translation="MKPVLCAFAVLVVAGCKDALPSGPAGVSGRLAFVSDRRFGQFDI
                     YFVNADGSGVSLLDSSLANDLWPSWSPDGKYVAYQSDRLLAPGATVAYYNIYVITADG
                     TGVVALTSDTLSNIQPAWSPDGMKIAFTTKRDGNYELYVMDAGGGNFVRLTNDHGEDG
                     QPAWSPDGSRIAFASDRSGNADIYVMNADGSNVVDLTNDGAEDLGPAWSPDGTKIAFS
                     SDRTGQSQIWVMDADGNNAVRRSGGSSPAELPAWSPTGDRLAYDSDGDIWLVWADGTS
                     AVRITKQLASDYSPRWRP"
     gene            complement(1798..2412)
                     /locus_tag="AC16JC_16915"
     CDS             complement(1798..2412)
                     /locus_tag="AC16JC_16915"
                     /inference="COORDINATES: protein motif:HMM:NF024853.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HIR5166435.1"
                     /translation="MRPTALLLAAGLLVPAGPAQQVDPLAARTKGSPTAPVTVYEMAD
                     FQCPACRQFALTTFPVIDRDYIQTGKVRWVFVNFPLSSIHKNAVAAAQVAMCAARQQR
                     FWQLHDALYAHQNEWAPLDQPLPQLIALADSAGVRHDSLLSCVNSRATVDAITQDTQG
                     SVRAGANATPSFYIEGGLAEGAIPAPAFARILDSIYRTKAGAPR"
     gene            2818..6114
                     /locus_tag="AC16JC_16920"
     CDS             2818..6114
                     /locus_tag="AC16JC_16920"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HIR5166436.1"
                     /translation="MTNALSTRFLRVARIVAAAAWLMAPPVFAQTGRIAGVVRDASTS
                     NVIQGAHVLVVGTSLTATTGADGAFALDSVPVGGVLVRVQAVGYQPASASIQVAAGAV
                     VTANFGLHQSIINLSEVVVTGVVGATERAKLPINVDRITSSDLPVPQLDPLTALQGKV
                     AGAMVTSGSGRPGSSPTILLRGPTSIDASGRTQEPLYVVDGVILGSDVVDLDALDIES
                     IEVVKGAAASSLYGSRAQNGVVQITTRRGASAPEEIHFTIRSEGGFNRLPSEFDLTTH
                     HQFALQNGLFLNQDPVTGDTITCAWLDCKQPPLFAGQTAAPGQAGNQWNTYQDVAWPG
                     QTFDQVKRFFQGGNFTQQYVAAEGRSGSTNFHASWTNAREEGIMAGQLGLWRNTLRMN
                     LDQWLGGKFKVAASTFYSRSKEDNRSGDLFGLTRMPAGVDLLTLNHCPATGTCAPWQQ
                     PRLLANGQQDPRDYYLNPDPFNNESPNPLYTSFNGSDFAYRGRFLASADGRWQPTGWL
                     AIDGNVSYDRLDWKQEVYTFKGYKTITPNPNTNNGNLSRNNSLTENLNASVDATFTRS
                     FGELATRTQFRYLAEYDNYDFTNATGRQFRVSDTPTIDNTDPALTTAGSGLTRVRADG
                     YFGITNLVFRDRYILDVLARNDGSSLFGPDARRHWYYRVAGAWRMTHDVRIPHMDELK
                     LRAAYGTAGGRPRFEAQYETYSVSGGQVTPVNLGNRNLKPEYSKELELGVDLLALGRV
                     GLTVNYASTVTQDQILQVPLPAFAGFQNQWRNAGTLESKTWEASLDLQLMRTQSVNWS
                     ARVLFDRTRQEITELDVPPFAYGAFGGNSGDVFFARRGEMIGTYYGVHVARSCADLLG
                     ALPCDQFAVNSDGLLVWVGPGGSLSNAQWGTAGPSFGFHGQRRTLMWGSPFVGWGIDP
                     ISHDTTNFLRVGKTNPDFHMGFSTSVGWHNLTLYGLLEWVKGISVYNVPQQWAVFRSY
                     AGIMDQSGVPDAQKKPLGYYNALYGLTSLAPDDYFVQDGSFAKLREVSLQYRLGRDQL
                     ARIGFLRSFDGIAFSVIGRNLLTFTSYNGYDPEVGSNGGATGSAAIARVDGYQYPNFR
                     NFAFSITVTY"
     gene            6144..>7213
                     /locus_tag="AC16JC_16925"
     CDS             6144..>7213
                     /locus_tag="AC16JC_16925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166437.1"
                     /translation="MSKATSGLTALLLIGMAGCANLDVANPNAPDAGRALSTAGDVEA
                     LIGGAFSRWTNVGDYNGPTMFLSNASGEHVAPWANAGMEMYARIPRIPTNNTAGAANV
                     SNLTFAWYESYGAIAAVHDGLKQINDGKVDLGADGDLRARAYGKFMQGLAHGTIALLY
                     DSAFVYDETSDPTAVSLQGYQDVMNAALGYLNDAITLAGSGTFTVPAAWMSQDVSSVT
                     LAQLAHSMAARLRAAVARTPAERAAVDWAQVVTDANAGITADWYITQDCNTGTFCNLF
                     NDAVIYRLVDGWQMQNNWVAGMADTSGAYQAWIATPLANKQPFIIHTPDARWPSGPDE
                     ATQLANPGDYYSMNSGGTRIWNR"
BASE COUNT         1219 a         2355 c         2465 g         1174 t
ORIGIN      
        1 cggggtgcac taaggacgcg accttcgtcg agccgattcc gccgctggcc gccatccatt
       61 tcgtgcacgc ggtgcccgac acgatgccga tggactttcg ggtcgtcgac atcgtgtcga
      121 acgccggcct gttcgacgcc agcttccgga ccgccaacat attctatacc gggatcgagg
      181 cgggcagccg acagattcgc gtcttcatca gcagcaccaa cccggccgtg acgggcgtgc
      241 cgatcttcga cacgaccttc gcgttcacgg cggacgcgga ctacaccttc attcacgcag
      301 gcttcgcgcg caccgggaac ccgccggcgc gcacggtctg gatcgtgccc gacaacgcca
      361 ccgcgcccgc ggcgggacaa gtcgggctgc ggctaatcca tgccggtgcc gggttggggg
      421 cactggacgt gaatgtgacg cggcacgcgg cggacacgct gcccgacgcg ccgctcgtgg
      481 gtggtctgac ctacagcacg gtgggcagct acctcgcgat gggtcgggac agcgtggcag
      541 cggactcgct gcgcctcgtg gtcaccgcgg caggcacgaa agcgcccgtg ctgttcagtc
      601 tgaaactacc ggccggcgcg gcgggcacga gctcagccga tcccatcgcc ggcgcccgcg
      661 ttgcgggttc ggtgatgact gcggtactgg tgcccccgtc cgtggtgggg agcgccgcgc
      721 ctcagggcgg ggcattcgcg gcgccgagcg ccgtgatcct ggtggaccgc cgtccgccga
      781 agaccacccc ctgagcggct gcacccgcac gccgagccca tccgcgcggg gaaacgccgc
      841 cgatcctcag catggtgggt cggcggcgtt cggttgttca ggctctaggg acgccagcgg
      901 ggactatagt ccgaggcgag ctgcttggtg atgcgaacgg cgctcgtgcc gtcggcccag
      961 acaagccaaa tgtcaccgtc gctgtcatag gcgaggcggt cgcccgtcgg cgaccaggca
     1021 gggagctcgg cgggggacga cccgccgctg cggcgcaccg cgttgttgcc atcggcgtcc
     1081 atgacccaga tttgcgactg gccggtccga tcgctcgaga aggcgatctt cgtgccgtcg
     1141 ggtgaccagg cgggtccgag gtcctcggca ccgtcgttgg tcaggtcgac cacgttggat
     1201 ccgtcggcgt tcatcacgta gatgtcggcg ttgccggacc gatcgctcgc gaacgcgatc
     1261 ctgctcccgt cgggcgacca tgccggctgc ccgtcttcgc cgtggtcgtt ggtgaggcgc
     1321 acgaagttgc cgccgcccgc atccatcacg tagagctcgt agttgccgtc gcgtttggtc
     1381 gtgaacgcga tcttcatacc gtccggcgac caggccggct ggatgttgct cagggtgtcg
     1441 ctcgtcaggg cgaccacgcc tgtgccgtca gcagtgatga cgtagatgtt gtagtaggcc
     1501 acggtggcgc cgggcgcaag caggcgatcg gactggtagg cgacgtactt cccatcgggt
     1561 gaccagcttg gccacaggtc gttggcgagg ctgctgtcca gcaggctcac gcccgagccg
     1621 tccgcgttca cgaaatagat gtcgaactgg ccgaacctgc ggtcgctcac gaacgcgagc
     1681 cggcccgaga cgcccgccgg accggaggga agcgcgtcct tgcacccggc cacgaccagg
     1741 acggcaaacg cgcacagcac aggtttcacg acggactccg ggtggagagc ggcgacgtca
     1801 gcggggtgcc cccgctttgg tgcgatagat cgagtcgagg atgcgcgcga atgccggcgc
     1861 ggggatcgcc ccctcggcga gcccgccctc gatatagaag ctcggggtgg cgttggcgcc
     1921 ggcgcggacc gaaccctgcg tgtcctgggt gatggcgtcg acggtggcgc gcgagttgac
     1981 gcagctcagg agagaatcgt gccgcacccc ggccgagtcg gccagcgcga tgagctgcgg
     2041 cagcggctgg tccaggggcg cccattcatt ctgatgcgcg tacagcgcgt cgtgcagttg
     2101 ccagaaccgc tgctggcgcg ccgcgcacat ggcgacctgg gcggccgcca ccgcgttctt
     2161 gtggatcgag ctgagcggga agttcacgaa tacccagcgc accttccccg tctggatgta
     2221 gtcgcggtcg atcaccggaa acgtggtgag cgcgaactgc cggcaggcgg gacactggaa
     2281 atccgccatc tcgtacaccg tgaccggcgc cgtggggctt cccttggtac gggcggcgag
     2341 cgggtcaacc tgttgggcgg gaccagccgg cacgaggagg ccggccgcga gcagcagggc
     2401 ggtgggtcgc atgcttctac aataatcggc ggagccggct ttcgcgccga cggggtggga
     2461 gtgggggcgg agggtgggag tggccggggt cgtgaggcag gggtctagcg ggtgcttgtg
     2521 gaacgtggta tatggtcagt ggggtggcgc acttagcgcg tcactccgac ctcgtggcga
     2581 catcctcccc agccgttggg tgtcggcgcg tgctcacacg aagacgacga gaggaaccgc
     2641 ccggggcgca cgtgacgcgc tccctggcct ggctgttact cggaggtatt tcccgcatgg
     2701 ccgtcatccg ggaagtgctc gacgtgatcc cggctcatct tttccctccg cgccgcggag
     2761 gcggttttgt cacgcccgtc ccaggtccag tgtgtccccc aacgcaggaa ggtgcgtatg
     2821 actaacgcgt tgtccacccg tttcctccgg gtcgcacgga tcgtcgcggc cgccgcgtgg
     2881 ttgatggcgc cgcccgtgtt tgcccagact ggacgcatcg caggtgtcgt gcgcgatgcg
     2941 tccacgtcga acgtcatcca aggggcccac gtcctggtcg tcggaacgag cctcaccgcg
     3001 acgaccggag ccgacggggc gttcgccttg gacagtgtgc cggtcggtgg cgttctggtc
     3061 cgggtccagg cggtcggcta ccagccggcg tcggcctcca tccaggtcgc ggccggtgcc
     3121 gtcgtcacgg cgaacttcgg gctgcaccag tcgatcatca atctctccga ggtcgtggtg
     3181 accggcgtgg tgggggcgac cgagcgggcc aagctcccga tcaacgtgga ccggatcacg
     3241 tcgtccgatc tgccggtgcc gcagctcgat ccgctgacgg ccctgcaagg gaaggtggcc
     3301 ggtgccatgg tgacgagcgg ctcgggacga ccgggaagct cgccgacgat cctgctgcgc
     3361 gggccgacga gcatcgatgc atcgggccgg acccaggagc cgttgtacgt cgtcgatggg
     3421 gtcatcctgg gatccgatgt cgtcgacctc gacgcgctcg acatcgagtc catcgaagtg
     3481 gtgaaggggg cggcggcgtc ttcgctgtac gggtcgcgtg cgcagaacgg cgtcgtgcag
     3541 atcaccacgc ggcgcggtgc cagtgcgccc gaggagatcc acttcacgat ccgttccgag
     3601 ggcggcttca acagactgcc gtcggagttc gacctcacga cacatcacca gttcgcgctg
     3661 cagaacggct tgttcctcaa tcaggacccg gtgaccggcg acacgatcac gtgtgcatgg
     3721 ctggactgca aacagccgcc gctcttcgcg gggcaaactg ccgcgccggg gcaggcaggt
     3781 aatcaatgga acacgtatca ggacgttgcc tggccgggcc agacgttcga ccaggtgaag
     3841 cgatttttcc agggcgggaa cttcacgcag cagtacgtgg ctgccgaagg ccgctccggc
     3901 agtacgaact ttcacgcctc atggaccaac gcccgtgagg aaggtatcat ggccggccag
     3961 ttgggtttgt ggcgcaatac cctgcgcatg aacctggatc aatggctggg gggcaagttc
     4021 aaggtcgcgg ccagcacgtt ctacagccgg tccaaggagg acaaccgcag cggcgacctg
     4081 ttcggcctca cccggatgcc cgccggggtc gacctgctca ccttgaacca ctgcccggcg
     4141 acggggacgt gcgcgccatg gcagcagccg cgcttgctgg ccaacggcca gcaggatccg
     4201 cgcgactact acctcaaccc ggacccgttc aacaacgaga gtccgaaccc gctctacacc
     4261 tcgttcaacg gcagcgactt cgcctatcgc ggccggttcc tggcgagcgc cgatgggcgg
     4321 tggcagccga ccggctggct cgcgatcgac ggcaacgtga gctatgaccg gctcgactgg
     4381 aagcaggagg tctacacctt caaggggtac aagacgatca ccccgaaccc gaacaccaac
     4441 aacggcaatc tgagccgcaa caactcgctg acggagaatc tcaacgcgag cgtggatgcg
     4501 acgttcaccc ggtcgttcgg ggagctcgcg acacggacgc agttccgcta cctcgccgag
     4561 tacgacaact acgatttcac gaacgccacc ggccgccagt tccgcgtgag cgacacaccg
     4621 acaatcgaca acaccgaccc cgcgctcacc accgccggat cgggattgac acgggtgcgg
     4681 gcggacggct acttcgggat caccaacctc gtcttccggg accgctacat cctcgacgtc
     4741 ctggcccgca acgacggcag ctccctgttc gggcccgacg cccggcgcca ctggtactac
     4801 cgggtcgccg gcgcgtggcg gatgacgcac gacgtgcgga ttccccacat ggatgagctg
     4861 aagctgcggg cggcgtacgg cacggccggt gggcggcctc gcttcgaagc ccagtacgaa
     4921 acgtattcgg tgtccggcgg ccaggtgacc ccggtcaacc tgggcaaccg caatctcaag
     4981 ccggagtact cgaaggagct ggaactgggc gtcgacctgc tggcgttggg gcgggtcggg
     5041 ttgacggtga actacgccag cacggtgacc caggaccaga tcctccaggt gccgcttccc
     5101 gcgttcgccg ggttccagaa ccagtggcgc aacgccggga cgctcgagag caagacctgg
     5161 gaggcctcgc tcgacctgca gctcatgcgg actcagtcgg tcaactggtc ggcgcgcgtg
     5221 ctattcgacc ggacccgcca ggagatcacc gagctcgacg tgccgccgtt tgcctatgga
     5281 gcgttcgggg ggaacagcgg cgacgtgttc ttcgcccgca gaggcgaaat gatcggcacc
     5341 tactacggcg tgcatgtggc ccggtcctgc gccgacctgc tcggcgcgtt gccctgcgac
     5401 cagttcgcgg tcaacagcga cggcttgctg gtctgggtcg gcccgggcgg cagtctgtcc
     5461 aacgcgcagt gggggaccgc aggaccgtcg ttcgggttcc acggacagag gcggaccctc
     5521 atgtggggct ccccgttcgt cggctggggg atcgatccga tcagccacga cacgaccaac
     5581 ttcctgaggg tcggcaagac gaaccccgat ttccacatgg gattctccac gtcggtcggg
     5641 tggcacaacc tgacgctgta cgggctgctc gagtgggtaa agggcatctc ggtgtacaac
     5701 gtgccacagc agtgggcggt gttccggagc tacgccggca tcatggacca gagcggtgtt
     5761 cccgacgcgc agaagaagcc gctcggctat tacaacgcgt tgtacggact gaccagcctc
     5821 gccccggacg attacttcgt tcaggacgga agcttcgcga agctgcgtga agtgtcgctc
     5881 caataccggt tgggccgcga ccagctggcc cgcatcgggt tcctgcggag cttcgacggg
     5941 atcgccttca gcgtgatcgg ccgcaatctg ctgacgttca cgagctacaa cggctacgat
     6001 cccgaggtgg gtagcaacgg cggggcaacc ggatccgccg cgatcgcccg ggtggacgga
     6061 taccagtatc cgaacttccg gaactttgcg ttctcgatca ccgtcaccta ctgagcggga
     6121 ggacacatcc catgtgccgg agaatgagca aggcgacatc gggtctgacg gccctcctgc
     6181 tgatcggcat ggcgggctgc gccaatctgg acgtggcgaa tcccaacgcc cccgacgcgg
     6241 ggcgggccct ctccacagcc ggcgacgtgg aggcgctcat cggcggcgcg ttcagccgct
     6301 ggacgaatgt gggtgactac aacgggccga ccatgttcct cagcaatgcg tccggcgagc
     6361 atgtcgcgcc ctgggcgaac gccgggatgg agatgtacgc ccgcatcccc aggatcccga
     6421 ccaacaacac cgccggagcg gcgaacgtca gcaacctcac gttcgcctgg tacgagtcct
     6481 acggggcaat cgccgccgtg cacgacggct tgaagcagat caatgacggg aaggtggacc
     6541 tgggcgcgga tggcgacctg cgcgcgcggg cctacgggaa attcatgcag gggctcgccc
     6601 acggcaccat cgccctgctg tacgactccg cattcgtcta tgacgagacg agcgacccca
     6661 cggcggtcag tcttcaaggg tatcaggacg tcatgaacgc cgcgctcgga tatctcaacg
     6721 acgcgatcac cctcgctgga agtgggacct ttacggtccc ggcggcgtgg atgagtcagg
     6781 acgtttccag tgtgaccctt gcccagctgg cgcactccat ggccgcgagg ttgcgcgctg
     6841 ccgtggcgcg cacgccggcg gagcgggcgg ccgtggattg ggcccaggtg gtgaccgacg
     6901 ccaatgcggg gatcaccgca gactggtata tcacccagga ctgcaacacc ggcacgttct
     6961 gcaacctgtt caatgacgcc gtcatctacc ggctggtcga tggctggcag atgcagaaca
     7021 actgggtggc gggcatggcc gatacctccg gcgcatacca ggcgtggatt gccaccccgc
     7081 tggcgaacaa acagccgttc atcattcata caccggatgc gcggtggccg agcggccccg
     7141 acgaagcgac ccagctggcg aacccgggcg actattacag catgaactcg ggcggcactc
     7201 ggatctggaa ccg
//
LOCUS       DBNGZW010000720         4370 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1415048, whole genome shotgun sequence.
ACCESSION   DBNGZW010000720 DBNGZW010000000
VERSION     DBNGZW010000720.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4370)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4370)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4370
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1415048"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..285
                     /locus_tag="AC16JC_16930"
     CDS             <1..285
                     /locus_tag="AC16JC_16930"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HIR5166438.1"
                     /translation="RKAVHSEFFHPPRLDDRLRVATYVGRVGTTSMTLNFDVLRDGAP
                     ALAAAGWMVLVCVDRQRLRPRPLPPALIEALGPHTLSLAAARTALGVADA"
     gene            282..785
                     /locus_tag="AC16JC_16935"
     CDS             282..785
                     /locus_tag="AC16JC_16935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166439.1"
                     /translation="MRRHAAGCLAVALAACGNPADPHGTGRVHLAVQGTTDTVRFEVP
                     VMAWRCAGGGGVLVTGAREGQGVLLLLRSAEPVVDTGSYVLLTRADSAALRGAIVAIR
                     FSVGPTSHGLTVDDGTARVSRTTPTLAAGVTGRGVETGTAVQRTVDLTVDDVPLAPDS
                     AFCRVQP"
     gene            782..1846
                     /locus_tag="AC16JC_16940"
     CDS             782..1846
                     /locus_tag="AC16JC_16940"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR5166440.1"
                     /translation="MTVRVGMIGTGFARRVQLPGLRLVPGVQVTAIASGRRANAEAVA
                     GEFGIARVYDDGAELARADDVDLVIISSTPPGHVRFAVAALEAGKHVLCEKPMALDAA
                     EAEHMALVAERRPSQVAWIDHELRYEPNRRKVRELVRAGAIGEVRHIELSLKPYLRGD
                     GRPQTASAPWTWWFDAAQGGGLLGAVGSHLIDLCRFWTGTEVVGVAGGVRTFVRERSD
                     AQGVRHEVTAAEFVSGVLTLSGGVVATLTLSAAAHHGPGHLAQVSGRDGSLVLTGEGR
                     LEIGRPGAALEDISVADDLAGRTSVNNMWARSFVRLARDLVSVIGGNPPEGEPAGFRD
                     GWRVQRVLDALRTGRPAPLD"
     gene            1851..3041
                     /locus_tag="AC16JC_16945"
     CDS             1851..3041
                     /locus_tag="AC16JC_16945"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HIR5166441.1"
                     /translation="MEQTYFRKGFGLRDAIEGALTTDYHSRLVDGIRANDHTLVLGDL
                     TFRLAREFGFCYGVDRAVEYAYEARSKFPERRAFLVGEIIHNPHVNSKLRAMGVEFLF
                     RGSGGDFDFSAVTQTDVVILPAFGVTIQDFARLREIGCVLVDTTCGSVLNVWKRVESY
                     ARDGYTAVIHGKYFHEETKATASQVTKYAAGKYIIVRHMDEAREVCAFLEGTVSRTQL
                     LERFAHAVSPGFDPDRDLVKIGVANQTTMLSNESLAIAGELQASMARRWGEAALADHF
                     RTFDTICSATQDRQDAVLTLIEEPLDAMVVIGGYNSSNTTHLAAICAPRVATYHVEDA
                     LCIDPQTGSIRYLPVGQREEARRDTWLPPGLVRIGITAGASTPNNKIGETVERIAAMR
                     GLSP"
     gene            3038..>4370
                     /locus_tag="AC16JC_16950"
     CDS             3038..>4370
                     /locus_tag="AC16JC_16950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5166442.1"
                     /translation="MNARFHPVVDRWFAARFAEPTEPQRRAWPLLQARRDVLIAAPTG
                     SGKTFAAFLAAIDDLLRQGLDGTLPDETQVVYVSPLKALSNDIQKNLSQPLEEIRRAL
                     EAAGLPDANLRVLLRTGDTPPADRQAMVKRPPHILVTTPESLYLILTSERARETLRNV
                     RTVIVDEIHAVARDKRGSHLALSLERLAALSQHPLQRIGLSATQKPIEEIAQFLVGTE
                     RPLPVIVDTGHARQLDLALEIPSSPLEAVISAEVWEEVDERLTQLIVEHRTTLVFVNT
                     RRLCERLTMQLSERLGADQVTSHHGSLSREKRLEAETRLKNGELKALVATASLELGID
                     IGSVDLVCQIGTPRAIATLLQRVGRSGHRLGAVPKGRIFPLSRDELIECAALLRAARE
                     GKLDRLIIPEHPLDILAQQVVAAVACEEWDEDALYDLVRRAYPYRDLSRKDY"
BASE COUNT          646 a         1491 c         1566 g          667 t
ORIGIN      
        1 cggaaagcgg tgcacagcga gttcttccat cccccccggc tggacgaccg gctgcgggtg
       61 gcgacgtacg tcgggcgggt gggcacgacc tcgatgaccc tcaatttcga cgtgctgcgg
      121 gacggtgcac ccgcgctcgc cgccgccggc tggatggtgc tcgtctgcgt ggaccgccag
      181 cgcctccgcc cacgccccct gccgcccgcg ctgatcgaag cgctcggccc gcatacgctc
      241 agcctggccg ccgcgcggac ggcgctcggc gtggcggacg cgtgaggcgc cacgccgccg
      301 gctgcctcgc tgtcgcgctc gcggcgtgcg gcaaccccgc cgatccgcac gggaccgggc
      361 gggtgcacct cgctgttcag ggaaccacgg acaccgtccg attcgaagtc cccgtcatgg
      421 cgtggcgctg tgccggtggt ggcggcgtgc tcgtgaccgg cgcccgggag ggtcaaggcg
      481 tgctgttgtt gctccggtct gcggagcccg tcgtcgacac cgggtcgtac gtgctgttga
      541 cccgggccga cagtgccgcc ctgcggggag cgatcgtggc gattcgattc tcggtcgggc
      601 cgacgtctca cggcctgacg gtggacgacg ggactgcccg ggtttcgcgg acgacgccga
      661 cgctggccgc cggcgtgacc ggacgtggag tggaaaccgg aacggcagtg cagcgcacgg
      721 tggatctcac ggtggacgac gtcccgctcg cgcccgattc cgcgttttgt cgcgtccagc
      781 catgacggtc cgcgtgggga tgatcggcac ggggttcgcg cgacgagtgc agctgcccgg
      841 cctgcgcctg gtgccgggcg tacaggtcac ggcgatcgcg agcgggcggc gcgccaacgc
      901 ggaggcggtg gccggggagt tcggcatcgc gcgtgtctat gacgacgggg ccgagctggc
      961 gcgcgcggac gacgtggatc tcgtcatcat ctcctctact cctcccggtc acgttcgctt
     1021 cgccgtcgcg gcgctcgagg ccgggaagca cgtgttgtgc gagaagccga tggcgctcga
     1081 cgccgccgag gcggagcaca tggcgctcgt ggcggagcgg aggccgtcgc aggtggcctg
     1141 gatcgatcac gagctgcgct acgaaccgaa ccgccgcaag gtccgcgagt tggtgcgtgc
     1201 cggcgccatc ggcgaggtcc gccacatcga gctatcgctc aagccctacc tgcgtggtga
     1261 cggccggccg cagaccgcga gtgcgccgtg gacctggtgg ttcgatgccg cccagggtgg
     1321 cggcctgctc ggtgcggtag ggtcgcacct gatcgacctg tgccggttct ggacgggcac
     1381 cgaggtggtg ggcgtcgccg ggggagtccg gacgttcgtt cgcgagcggt ccgatgccca
     1441 gggcgtgcgg cacgaagtga ccgccgccga attcgtgtcc ggggtcctga ccctgagcgg
     1501 aggcgtggtc gcgacgctca ccctctccgc cgccgcgcat cacggcccgg gccacctggc
     1561 ccaggtgtcg ggtcgagacg gctcgctcgt gctcaccggt gagggacggc tggagatcgg
     1621 tcgcccggga gcggcgctcg aggacatcag cgtggccgac gacctcgccg gccggacttc
     1681 ggtcaacaac atgtgggcgc gttccttcgt gcggctcgcg cgcgatctcg tgtccgtgat
     1741 cgggggcaac ccgcccgaag gcgagccggc cggcttccgc gacggctggc gtgtgcaacg
     1801 cgtgctcgat gccctgcgca ccgggaggcc ggcgccgcta gattgacctc gtggagcaga
     1861 cgtacttccg caagggcttc gggctcaggg acgccatcga gggcgcgctc acgaccgact
     1921 atcacagccg gctcgtcgac ggcatccgcg ccaacgacca cacgctggtc ctgggggacc
     1981 tgaccttccg cctggcgcga gagttcgggt tctgctatgg agtggaccgg gccgtcgaat
     2041 atgcatacga ggcccgcagc aagttccccg agcggcgcgc cttcctggtg ggcgagatca
     2101 tccacaaccc gcacgtcaac tcgaagcttc gggccatggg cgtcgagttc ctgtttcgtg
     2161 gttccggggg cgatttcgat ttcagcgcgg tgacgcagac cgacgtggtc atcctgccgg
     2221 ccttcggggt caccatccag gacttcgccc ggctgcggga gatcggctgc gtgctggtcg
     2281 acacgacttg cgggtcggtg ttgaacgtct ggaagcgagt cgagtcctac gcacgcgacg
     2341 gatacacggc ggtgatccac ggcaagtact tccacgagga gacgaaagcc accgccagcc
     2401 aggtgaccaa gtacgcggcg gggaagtaca tcatcgtccg gcacatggac gaggcacgcg
     2461 aggtatgcgc gttcctcgag gggaccgtga gtcgtacgca gctgctcgag cgcttcgctc
     2521 acgccgtctc gcccggcttc gacccggacc gcgacctcgt gaagatcggc gtcgccaacc
     2581 agacgacgat gctctccaac gaatcgctgg cgatcgccgg ggagctgcag gcaagcatgg
     2641 cgcggcgctg gggggaggcc gcgctggcgg accacttccg caccttcgac accatttgct
     2701 cggccacgca ggaccggcag gatgcggtgc tcacgctgat cgaggaaccg ctcgacgcga
     2761 tggtcgtgat cggcggatac aactcgagca acaccacgca cctcgccgcc atctgcgcac
     2821 cgcgcgtcgc gacctaccac gtcgaggacg cgctctgcat cgacccgcag accgggagca
     2881 tccggtacct gccggtgggg caacgtgagg aagcgcggcg tgacacgtgg ctgccgccag
     2941 ggctggtccg catcggcatc acggccgggg cctccacccc gaacaacaag atcggggaga
     3001 ccgtcgagcg catcgccgcg atgcgcgggc tctcgccatg aacgcccggt tccatccggt
     3061 cgtcgaccgc tggttcgccg cgcgattcgc ggagccgacc gagccacagc gtcgcgcctg
     3121 gccgttgctc caggcccggc gcgacgtcct gatcgccgct cccaccggct cgggcaagac
     3181 cttcgcggcc ttcctcgccg cgatcgacga tctgctgcgt caggggctgg acggcacgct
     3241 gcccgacgaa acgcaggtcg tctacgtgtc gccgctcaag gcgctctcca acgacatcca
     3301 gaagaacctg tcgcagccgc tggaggagat tcggcgggcg ttggaggccg cagggctccc
     3361 cgacgcgaac ctgcgcgtcc tgctgcgcac gggcgacacg ccgcccgcgg acaggcaggc
     3421 gatggtgaag cggccaccgc acatcctggt caccaccccg gagtcgctct acctcattct
     3481 cacttcggag cgcgcgcgcg agacgttgcg caacgtgcgc accgtgatcg tggacgagat
     3541 ccacgcggtg gcgcgggaca agcggggcag ccatctcgcc ctgtcgctcg agcggctggc
     3601 ggccctgtcg cagcatccgc tgcagcgcat cggcctgtcg gccacccaga agccgatcga
     3661 ggagatcgcg cagttcctcg tggggacgga gcgaccactg ccggtcatcg tggatacggg
     3721 tcacgcgcgg cagctcgatc tggccctgga gattccctcc tcgcccctcg aagcggtgat
     3781 ctcggccgag gtctgggaag aggtggacga gcgactgacg cagctgatcg tcgagcaccg
     3841 gacgacgctg gtgttcgtga acacgcgacg gctgtgcgag cgcctcacga tgcagctctc
     3901 cgagcggctc ggcgcggacc aggtaacatc gcaccacggt agcctgtcac gcgagaagcg
     3961 tctcgaggcc gagacccgtc tcaagaacgg cgagctgaag gcactggtgg cgaccgcctc
     4021 gctcgagctg ggaatcgaca tcgggtcggt ggatctcgtc tgccagatcg ggacgccgcg
     4081 cgcgatcgcg acgctgttgc agcgcgtggg ccgctcgggg caccgcctgg gcgccgtgcc
     4141 gaagggtcgg atcttcccgc tgtcgcgcga cgagctgatc gagtgcgccg cgctgttgcg
     4201 ggccgcgcgc gaaggaaagc tggaccggct gatcatcccc gagcaccctc tcgacatcct
     4261 cgcgcagcag gtcgtggcgg cggtcgcctg cgaggagtgg gacgaagacg ccctgtatga
     4321 cctcgtgcgc cgggcttacc cgtaccggga cctgtcgcgg aaggactacg
//
LOCUS       DBNGZW010000721         2360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1415151, whole genome shotgun sequence.
ACCESSION   DBNGZW010000721 DBNGZW010000000
VERSION     DBNGZW010000721.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1415151"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(70..741)
                     /locus_tag="AC16JC_16955"
     CDS             complement(70..741)
                     /locus_tag="AC16JC_16955"
                     /inference="COORDINATES: protein motif:HMM:NF014373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HIR5166443.1"
                     /translation="MQDAIARMVDALQLGLLLQLGLAVLCGGAIGMERELSGKPAGLR
                     TNILICVGATLFTVLSLKLAGVRGDPARIAAQILPGVGFIGAGTILHARGAVTGLTSA
                     ATIWVVAAIGMALGGGAYTEALGTTLLVTLVLAGLGWVETHVARRATHSRLQIHAKPE
                     TSTLEELETLVRRTGLELTRIESRRENVDLVIELEVRGPKRLHDQAMIAILHHAGVRS
                     VSTGE"
     gene            complement(741..1742)
                     /gene="trpS"
                     /locus_tag="AC16JC_16960"
     CDS             complement(741..1742)
                     /gene="trpS"
                     /locus_tag="AC16JC_16960"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR5166444.1"
                     /translation="MAHPRVFSGIQPSGELHIGNYLGAVKNWVRLQDQHECVICIVDL
                     HAITQAYEPAELAQRTHDMAVGLLAAGIDPARSILFVQSHVVEHTELCWILTTVTPLG
                     ELERQTQFKDKSQRQESVPAGLLNYPVLQAADVLLYRAALVPVGEDQLQHLELMREIA
                     RRWNNRYGAGFFPEPQAQLTSTKRVLGIDGQAKMSQSLGNTIGLLESPEAIWEKLRPA
                     ATDPARKTRKDPGNPDICNIFTIHQGFSPPAVQAEVAQNCRTAAWGCLDCKRVLADHM
                     IAELTPIRERAAALQANPGRVRELLRQGAERARTMARATMAEVRRRMGFLDAAGGAD"
     gene            1805..>2360
                     /locus_tag="AC16JC_16965"
     CDS             1805..>2360
                     /locus_tag="AC16JC_16965"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR5166445.1"
                     /translation="MNAAERAAERAANYVRGARAPEAGAWTEKSRHDFVTQVDREAEE
                     LIAETLTGLVPGTVVVGEELSPAAEYRGEVVWVVDPLDGTTNYLHGYPQYAVSIGALV
                     DGVLSVGVVHDVARDVAYRAARGHGAWCGESRLAVSALVEPSRALVGTGFPFKHLEGM
                     EQYLRQFAAVVRTTSGIRRAGSAAL"
BASE COUNT          348 a          812 c          834 g          366 t
ORIGIN      
        1 gccccaccac ctccggggtg acgcgccaca cgggccgctg ggcccgcatg tccacgacga
       61 gtcgttgggt cactctccgg tcgagaccga gcgcacgcct gcgtgatgca ggatggcaat
      121 catcgcctga tcatgcaggc gcttgggccc cctgacctcg agctcgatca ccaggtcgac
      181 attctcccgg cgggactcga tgcgcgtgag ttcgagtcct gtacggcgca cgagcgtctc
      241 gagctcctcc agggtggacg tctccggctt ggcgtggatt tgcagccgcg agtgcgtcgc
      301 acgccgggcc acgtgggttt ccacccaccc gaggcccgcg agcacgagcg tgacgagcaa
      361 cgtcgtcccc agcgcctcgg tgtaggctcc gccgcccagc gccatgccga tcgccgccac
      421 cacccagatc gtggccgcgc tcgtgagccc cgtgaccgcg ccgcgggcgt gcaggatcgt
      481 gcccgccccg atgaagccca cgccgggcag gatttgtgcg gcgatgcgcg cgggatcgcc
      541 ccgtacgccg gcgagcttca acgacaggac agtgaacaag gtcgcaccga cgcagatcag
      601 gatgttggtg cgcagcccgg ccggcttgcc cgacagctcc cgctccatcc cgatcgctcc
      661 gccgcacagc accgccaggc ccagctggag cagcagaccc agctgcagcg catccaccat
      721 ccgcgcgatc gcgtcctgca tcaatccgcc ccgcctgccg cgtcgaggaa ccccatgcgg
      781 cggcgtactt cggccatcgt cgcgcgggcc attgtccggg cccgctcggc cccctggcgc
      841 agcagctcgc gcacgcgccc cggattcgcc tgcagcgcgg cagcgcgctc gcggatcggt
      901 gtgagctcgg ctatcatgtg gtccgccagc acccgcttgc agtcgagaca cccccacgcc
      961 gccgtgcgac agttctgcgc gacctcggcc tggaccgcgg gcggcgagaa cccctggtga
     1021 atggtgaaga tgttgcagat gtcggggttg ccgggatcct tgcgcgtctt gcgcgccggg
     1081 tcggtggcgg cgggccgcag cttctcccag attgcctcgg gcgattcgag cagtccgatc
     1141 gtgttgccga ggctctgcga catcttggcc tgtccgtcga tccccagcac ccgtttggtg
     1201 gatgtcagct gcgcctgggg ctcggggaag aagccggcgc cgtagcggtt gttccagcgg
     1261 cgcgcaatct cgcgcatcag ctccaggtgc tgcagctggt cctcgcccac cgggaccagg
     1321 gcggctcgat agagcaggac gtcggcggcc tggagcacgg ggtagttgag caatccggcc
     1381 ggcacgcttt cctggcgctg cgacttgtcc ttgaactgcg tctgacgctc cagctctccg
     1441 agcggggtga cggtggtgag gatccagcac agctcggtat gttccacgac gtgcgattgc
     1501 acgaacagaa tcgaccgggc cggatcgatt cccgccgcga gcaggcccac cgccatgtcg
     1561 tgcgtgcgct gcgccagctc ggccggctcg tacgcctgcg tgatcgcgtg caggtcgacg
     1621 atgcagatga cgcactcgtg ctgatcctgg agccgcaccc agttcttcac ggcgccgagg
     1681 tagttgccga tgtgcagctc gccggacggc tggatgccgc tgaagactcg ggggtgggcc
     1741 atatggggtc acaacctaag agggcggagg gaagagccgc aaggtgcgga ccctcaagga
     1801 cttagtgaac gccgccgagc gcgccgccga acgggcggcg aactacgtgc gcggtgcgcg
     1861 cgcgccggag gccggggcgt ggaccgagaa gtcgcggcac gacttcgtga cccaggtcga
     1921 ccgcgaagcc gaggagctga tcgcggagac gctcacgggg ctcgtgccgg ggaccgtggt
     1981 ggtgggcgag gagctctccc ctgccgcgga gtaccgcggc gaagtggtgt gggtcgtgga
     2041 cccgctggac ggcacgacca actacctgca cggctacccc cagtacgcgg tgtcgatcgg
     2101 cgccctggtg gacggcgtgc tgagcgtggg cgtggtgcac gacgtcgccc gcgacgtggc
     2161 gtaccgcgcc gcgcgaggcc atggcgcgtg gtgtggcgaa tcgaggctcg ccgtctcagc
     2221 actcgtcgag ccgagccgtg cgctggtggg caccggattc ccgttcaagc atctcgaggg
     2281 gatggaacag tatctgcggc aattcgcggc ggtggtgcgc accacgagcg gcatccggcg
     2341 cgcgggatcg gccgccctgg
//
LOCUS       DBNGZW010000722         9881 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_544390, whole genome shotgun sequence.
ACCESSION   DBNGZW010000722 DBNGZW010000000
VERSION     DBNGZW010000722.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 9881)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9881)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9881
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_544390"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..610
                     /locus_tag="AC16JC_16970"
     CDS             <1..610
                     /locus_tag="AC16JC_16970"
                     /inference="COORDINATES: protein motif:HMM:NF023444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pilus assembly FimT family protein"
                     /protein_id="HIR5166446.1"
                     /translation="STRLLGPLVGPGARRARPRVSGFSLIEMLIVLVVIGLLVGITAP
                     KIDVVKYRLEAGMQGVGMTLLAAERQAITQQHDVVVTFDVPNGAIHIFDDANNNGLKD
                     AGERERGMTLGEGVVFGRALAPARPMGAGPVTFTKMVNGLPALVFHRDGSASEGGGFY
                     LTSTRAVESGTHVEDTRSVEVERATGRATWYRYGPAAWRQAF"
     gene            612..992
                     /locus_tag="AC16JC_16975"
     CDS             612..992
                     /locus_tag="AC16JC_16975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification PilV family protein"
                     /protein_id="HIR5166447.1"
                     /translation="MSARRGFTLIEVILALTILLVVLMLLATSTGRAVHTAATSSAQA
                     AAQELAMDRLETIRADPRYDAIDTLYTRTETSFPTLPGYTRTTRIVRVGGIGQANDYK
                     RITVTVTGPGLTVPLSRSTTVAAP"
     gene            989..2155
                     /locus_tag="AC16JC_16980"
     CDS             989..2155
                     /locus_tag="AC16JC_16980"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR5166448.1"
                     /translation="MSAPRRGAEAGMTLIEMMIALVVFSFALAGTLAFLRAQGRSFTL
                     GSQRVAMLQNGRFAFRELEQDLRTAGAGAPDIQPPLIYLDTSVVAFNADYATNTPGDV
                     FAVYYTPDAPPTSTAAMTKEERTAIPLSAVSYPDTSYVTGLLNSPAETIVFFFAPDTS
                     TPRADDYVLYRRVNALAPEVVSRNLLHAPGVPFFQFYRLVVPAGGGESSIRQVPNDSL
                     PLWHSVPIHLTLADVGLAAQIDSVRGVQVTFAVTNGLSGAAERRRSLSRYIRFPNVGL
                     AVRKTCGDEPILGTPLTAVWNATSGGVDLTWNAAVDELAGERDVERYVIWRRLATDVA
                     WGDPHVSLSPTGTTPYLYTDRPVTSGARYVYALAAQDCTPSNSTLAVSAPVDIP"
     gene            2179..3978
                     /locus_tag="AC16JC_16985"
     CDS             2179..3978
                     /locus_tag="AC16JC_16985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166449.1"
                     /translation="MRTSRVFGNERGIALVVVLLVVLAVAAIVGGAALLGSGAALISR
                     HDARQSVLETAADAGLEEARSRINGARIVGDTTVFPDAGFRALETRATVVDAAGAPIP
                     NVNRWLYVGPSGVTTGQYGVFGSIVAVTQDAQGDRVIRRNEIFQESFSRYAYFTTVEG
                     NIFFANGDQIFGPVHSDDDLHIDPSGATFHGPVSTAKAIQGRANGVYKQGYREHAPYI
                     AMPTTADLAKLRTQAQIGGMAITSTTFGNQGQATTRIEFVAVDLDGDNDSTDADEGFM
                     KVYQVASPGNAWWVVADTFQWKANGVRNSPNCGHIDGGVFKTFESHTATSGTDAKSVA
                     PGDTTKPRACFLGGADSLNNLMAFASSDARGGWLPWTGTVDPRLTVVKHEEASYLWPI
                     NRALNPNFKGVIYVDGKVAVSGKLRGRVTVAATDNIVIADDITYVTDPGTVNCADILG
                     LFSGKAIVVADNLINDPIPWASGKDPTHWDADSWDESIDGFVLALDMFTAQNYNEGST
                     DAEPCGVKKAGRGCLFLTGGVIQTYRGAVGFIPAGSASGTGYIKRYAYDRCGATDPPP
                     YFPTTGHFLPGHSYEVDPTGFDINAYWKLLTPH"
     gene            4118..4492
                     /locus_tag="AC16JC_16990"
     CDS             4118..4492
                     /locus_tag="AC16JC_16990"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HIR5166450.1"
                     /translation="MMARPRGFTLVEVLVALLVFAVGALGLAAETAALTRLVARSRRA
                     ARVSAAAALRLERLQAGGCDARTSGTELVMYGGSALAALHWSWSPDGTGYEVRLVTAP
                     GPLTMGPAAPAETLRAVVSCAS"
     gene            4480..5346
                     /locus_tag="AC16JC_16995"
     CDS             4480..5346
                     /locus_tag="AC16JC_16995"
                     /inference="COORDINATES: protein motif:HMM:NF019575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HIR5166451.1"
                     /translation="MRVLRSRGVSLVELLVALTLSGLLAAVVAGVLGRTQNFYRAATQ
                     EIDVSQSLRVAGSFLPAELRELDAADGDVVAMAPTAVTIRAARQLAVLCRAPLPADSP
                     ASVTLTLRATPFSELRDFDPATDSLWIFREGDPLSPEDDDWIAAAFTTLEPDTCADGG
                     AGRRVTAFLRLAAGQTMDAGQVTAGAPVLGFETVTYRLYRSSADRRWYVGMETSSDLQ
                     PVLGPVTDDGLAFSYLDSTGATAAQAARVRAIEFRVRARTVEPVRTRDGRLDHPLDSL
                     FAVVFLRNNPRF"
     gene            5350..6426
                     /locus_tag="AC16JC_17000"
     CDS             5350..6426
                     /locus_tag="AC16JC_17000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166452.1"
                     /translation="MPCVATERRGIALPLVLWALVIGSALLTVAVFLILQEQRAGQAG
                     RRLIHAFTRAEAGLTEVLGAWTPGSLNRALPGVLDSLGVTGSDLAIRRLNQGLFLLAV
                     RVRDGATEARLGRLVRVRPVHLAVPAALTSGGPVRLGGEVTVSGADQPPAGSEDCPPV
                     DSAVAGVAAPAVTTTESTVITGSPPTVERQPADSGLAPEDLLVFRDLASQATVSLAGG
                     VLQTAPVTVGTVCSLSAAENWGDPFAPGGPCGAYMPIIYVTGDLDLLSGRGQGILLVE
                     GHLRVHGPYSFAGLIMARGGLDIATADSAVHLKGAVFAGSVGTQTTPPAVLSITYSKC
                     LVSKALLSSGQLVPLRTRSWKALF"
     gene            6560..7609
                     /gene="pilM"
                     /locus_tag="AC16JC_17005"
     CDS             6560..7609
                     /gene="pilM"
                     /locus_tag="AC16JC_17005"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HIR5166453.1"
                     /translation="MALFGIGAKKTSVGLDIGSGIVKLVVIDHRGAEPELVRVATTEV
                     AADAIVEGEIMDPGIVAEAIRGLFSTAGVKPKGIVTAVGGRDVIVKKIQMDRMREAEA
                     RDVIRWEAEQHVPFDMANVELDFQILDPEAEGLQMNVLLVAAKRELVEGRVSLLREAG
                     LEPGIIDVDAFAIHNAFELNYPEAMHGVVGLVNIGHEVTNVNILDDGVPVLTRDLSVG
                     TRRFREDLQREKGLSAEDSERVVTGAAQSADLSGYVEARGEEIAVGVERAAAFLATAS
                     RSAGGLARVFTSGGGARVPGLNEALAQRLKVPVEPANPVQRLKVRDTVYESVSLDEVA
                     PLLMLSVGLALRRAA"
     gene            7606..8307
                     /locus_tag="AC16JC_17010"
     CDS             7606..8307
                     /locus_tag="AC16JC_17010"
                     /inference="COORDINATES: protein motif:HMM:NF016992.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HIR5166454.1"
                     /translation="MIEINLLPGQKRKAAGGGGLRMPDFRSVLTNVKDPWLLGAIAAG
                     VILIGGNLALYLVNTARLARLDSVLRGVQAEKRRYDAVVAQKRQAERVRDSLAAEMVI
                     IRGIDGDRYIWPHLLDEITKALPAYTWLTGIQATSGVAPAVGLPSGAAPAADTGAGQG
                     VRVGIDGRTVDIQAYTTFLRQLAASPWLADVMPTKATTVIEQDRPVTEFNITVRYRQA
                     DSLYVRTVPLVQSVR"
     gene            8311..9000
                     /locus_tag="AC16JC_17015"
     CDS             8311..9000
                     /locus_tag="AC16JC_17015"
                     /inference="COORDINATES: protein motif:HMM:NF016253.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus inner membrane component PilO"
                     /protein_id="HIR5166455.1"
                     /translation="MALGIPQDSRSQVMLLIIVACLAGGYFFWTKIQSPKQASITATG
                     QEIDSLEAVVRKAKADLAGGSVESMRRSVERYRAALGLMRRLVPEQNEVPSLLEDISN
                     RAKVRGIQLGSFQPLSPEPGPPLALTYREPGDTGKKKAGAPAFDIYRYRLQVYGHYDQ
                     IGEFLADVSSLPRIMVPQALVLRAASQQTQKLVGDTLGALLEASFDLRTYVKRVPPST
                     SSAGGAIDARP"
     gene            8987..9424
                     /locus_tag="AC16JC_17020"
     CDS             8987..9424
                     /locus_tag="AC16JC_17020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166456.1"
                     /translation="MRVRSLLIAGCWLAVPAAGQTARADSARDSLARPDTAPLTLVRE
                     VFSYQGAGRDPFMSLLKSGDVRPLITDLKLVTVIYDERYAARSVVVLRDITTSKRYRA
                     RVGDVIGRLRVTQIRPREVVFTVQEFGFERQQTLSLAKQEENP"
     gene            9421..>9881
                     /locus_tag="AC16JC_17025"
     CDS             9421..>9881
                     /locus_tag="AC16JC_17025"
                     /inference="COORDINATES: protein motif:HMM:NF023169.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HIR5166457.1"
                     /translation="MKRSIVTIACLAVAAAPRLPADPTRDGEVRAISVAPASGRVDVV
                     IGLQGAVEVQDFTLEAPARLVIDLLGARLTAPAALYDGQNRGGVRNIRYAQFRPGVVR
                     VVIELDALADYQIQRTDGQVRVEIGTERTAFGAWSSSNTPRVAAAPAASEPA"
BASE COUNT         1539 a         3300 c         3452 g         1590 t
ORIGIN      
        1 gagtacccgt cttctcggac cgctggtggg gccgggcgcg aggcgcgccc ggccgcgggt
       61 gtctgggttc tccctcatcg agatgctcat cgtgctggtc gtgatcggcc tcctcgtcgg
      121 catcaccgct cccaagatcg acgtggtcaa gtaccggctg gaggcgggga tgcagggggt
      181 ggggatgacg ctcctcgccg ccgagcggca ggcgatcacc cagcagcacg acgtcgtcgt
      241 caccttcgac gtccccaacg gggcgatcca catcttcgac gacgccaaca acaacggcct
      301 gaaagatgcg ggcgaacgag aacggggcat gaccctgggg gaaggcgtcg tgttcggtcg
      361 cgccctggcg ccggcccggc ccatgggtgc cggtccggtc accttcacca agatggtcaa
      421 cggcttgccg gcgctggtgt tccaccggga cgggagtgcc agcgaaggcg gcggattcta
      481 tctcacctcc acccgggcgg tggagtccgg aacacatgtg gaggacacgc gctccgtcga
      541 agtcgaacgg gcgaccggcc gcgcgacctg gtaccgctac gggccggccg cctggcggca
      601 ggccttctga catgagcgcg cgccgcggct tcaccctgat cgaggtcatc ctcgccctga
      661 cgattctgct cgtggtgctc atgctgctgg cgaccagcac cggaagagcg gtgcacaccg
      721 ccgcgaccag ctcggcccag gcggcggcac aggagctggc gatggatcgc ctcgagacga
      781 tccgcgccga cccgcgctac gatgcgatcg acaccctgta cacacggacc gagacgtcct
      841 tccccacgct tcccgggtac acccggacga cgcggatcgt gcgcgtcggg ggaatcgggc
      901 aggccaatga ctacaagcgg atcacggtga cggtgaccgg gcccgggctc acggtgccgc
      961 tctcccgttc gacgaccgtg gcggcaccat gagcgccccg cgacgtgggg cggaggccgg
     1021 catgacgctc atcgagatga tgatcgcgct ggtcgtgttc tcgttcgcgc tggccggcac
     1081 gctcgccttc ctgcgcgccc agggacggag cttcaccctc ggcagccagc gggtggcgat
     1141 gctgcagaac ggccgctttg ccttccgcga gctggaacag gacctgcgga ccgccggcgc
     1201 cggggcgccc gacatccagc ccccgctgat ctatctcgat acgtcggtcg tcgcattcaa
     1261 cgccgactac gccaccaaca cgcccgggga cgtgttcgct gtctactaca ctcccgacgc
     1321 accgccgacc agcactgcgg cgatgaccaa ggaggagcgc acggcgattc cgctctcggc
     1381 cgtcagctac cccgacacca gctacgtgac cggcctgctc aacagtcccg cggagacgat
     1441 cgtcttcttc ttcgctcccg acacgagcac gccgcgggcc gatgactatg tgctgtaccg
     1501 gcgggtgaac gcgctggccc ctgaggtcgt gtcgcggaat ctgctccacg ccccgggcgt
     1561 cccgttcttt cagttctatc ggttggtggt accggcggga ggaggcgagt cctcgatccg
     1621 gcaggtgccg aacgatagcc tcccgctgtg gcattccgtt ccgatccacc tgaccctggc
     1681 ggatgtgggc ctggccgcgc agatcgacag cgtacgcggc gtgcaggtca cgttcgccgt
     1741 caccaacggg ctgagcggtg cggcggaacg gcgccggtcc ctgagccgct atatccggtt
     1801 cccgaatgtg ggactcgccg tcaggaagac gtgcggcgac gagccgatcc tggggacgcc
     1861 gctgacggcc gtctggaacg ccacgagcgg gggcgtcgat ctgacctgga acgccgccgt
     1921 cgacgagctg gcaggggaac gggacgtcga gcgctacgtg atctggcgcc ggctggcgac
     1981 cgacgttgcg tggggagatc cccatgtgag cctgtcgccg acggggacga ccccgtacct
     2041 gtataccgat cggcccgtga cctccggcgc ccggtacgtt tatgcgctgg cggcccagga
     2101 ctgcacccca tcgaactcga cgctcgcagt cagtgccccg gtcgacattc catgacggct
     2161 cgaggagagg acgaacgtat gcggacatcc cgggtgttcg gcaatgagcg gggcatcgcg
     2221 ctcgtggtcg tcttgctggt ggtgctggcc gtggccgcca tcgtcggcgg agcggcgctg
     2281 ctgggatccg gcgccgctct gatcagccgc cacgatgccc ggcagagcgt gctcgagacc
     2341 gccgcggacg cgggcctgga ggaggcgcgc tcgcgcatca acggcgcgcg catcgtcggc
     2401 gacacgacgg tgttcccgga cgcgggcttt cgcgcgctcg agacgcgagc caccgtggtc
     2461 gacgccgccg gcgcgccgat tccgaacgtg aaccgctggc tgtacgtcgg gccgagcggt
     2521 gtgaccacgg gccagtacgg ggtgttcggc agcatcgtcg cggttaccca ggacgcccag
     2581 ggggaccggg tcatccggcg caacgagatc ttccaggaga gcttctcgag gtacgcctac
     2641 ttcaccacgg tcgaaggcaa catcttcttc gcgaacgggg accagatctt cggcccggtg
     2701 cactcggacg acgatctgca catcgacccg tcgggcgcga cgttccatgg tcctgtctcg
     2761 acggcgaaag ccatccaggg gcgcgccaac ggcgtgtaca aacaggggta ccgggagcac
     2821 gcgccctaca tcgcgatgcc tacgaccgcg gacctcgcga agctgcggac tcaggcgcag
     2881 atcggcggca tggcgattac cagcacgacc ttcggcaacc agggccaggc cacgacccgc
     2941 atcgaattcg tggccgtcga tctcgacggc gacaacgatt cgaccgacgc cgacgagggc
     3001 ttcatgaagg tgtatcaggt cgccagcccc ggaaacgcct ggtgggtggt cgccgacacg
     3061 ttccagtgga aggccaacgg ggtgcgcaac tcgcccaact gcgggcatat cgatggcgga
     3121 gtattcaaga ccttcgagag ccacacagcg accagcggca cggacgccaa gtccgtcgcc
     3181 cccggagaca ccaccaagcc gcgtgcgtgc ttcctcggcg gtgccgacag cctcaacaat
     3241 ctgatggcct tcgcctcgag cgacgcgcgc ggcggctggc tgccctggac cggcacggtg
     3301 gacccgcgcc tgacggtggt caagcatgag gaggcttcgt atctctggcc catcaaccgc
     3361 gcgctgaacc ccaacttcaa aggcgtcatt tacgtcgacg ggaaggtcgc ggtgagcggc
     3421 aagctgcggg gccgggtaac cgtcgccgcg accgacaaca tcgtgatcgc cgacgacatc
     3481 acctacgtca ccgaccccgg aaccgtgaat tgcgccgaca tcctcgggct gttcagcggg
     3541 aaagccatcg tggtggcgga caacctcatc aacgatccca tcccctgggc atcgggcaag
     3601 gaccccaccc actgggatgc cgacagctgg gacgagtcca tcgacggatt cgtgctggcc
     3661 ctcgacatgt tcacggcgca gaactacaac gagggctcca ctgatgccga gccatgcggc
     3721 gtgaagaagg cgggccgcgg ctgcctgttc ctgacgggcg gggtgatcca gacctaccgc
     3781 ggagccgtcg ggttcatccc cgccgggtcc gcctccggca cagggtacat caagcgctat
     3841 gcgtacgacc ggtgcggcgc cactgaccca ccgccctact tcccgaccac cggtcatttc
     3901 ctgcccggac attcctacga ggtggacccc accgggttcg acatcaacgc gtactggaag
     3961 ctgctgaccc cgcactgatc gggtcgcacg tccggcacgg ggtcgcgtgg cccggcgtca
     4021 gcaacggcgc cgggcttctt gttggtcgcg acgaaacagc ccggctcgcg ttgtttggcc
     4081 atcgatcgcc cgtagggact ttcgtatcat cgcccccatg atggcccggc cgagggggtt
     4141 caccctggta gaggtgctgg tcgcgttgct cgtcttcgcg gtcggcgccc tcggcttggc
     4201 ggccgagacc gcggcgctga ctcgcctggt ggcacgctcg cggcgcgcgg cccgggtatc
     4261 ggcggcggcc gccctgcgtc tggagcggct ccaggccggc ggctgcgacg cccgcacgag
     4321 cggcaccgag ctcgtcatgt acggcggcag cgcgctcgcc gcactgcatt ggagctggag
     4381 cccggacggc acgggctacg aggtgcgcct cgtgacggct cccggtcccc tgaccatggg
     4441 accggccgct ccggccgaaa cgctcagggc ggtggtctca tgcgcgtcct gagatcgcgc
     4501 ggcgtatcgc tggtggagct gctggtcgcg ctgacactgt ccggcctcct cgccgcggtc
     4561 gtggcggggg tactcggtcg cacgcagaac ttctatcgcg ccgccacgca ggaaatcgat
     4621 gtcagccaga gcctgcgcgt ggccgggtcg tttctcccgg cagagctgcg ggagctggac
     4681 gccgcggatg gcgatgtcgt ggccatggcc ccgaccgccg tcaccatccg cgcggcccgc
     4741 cagctggcgg tcctgtgccg cgcgccgctg ccggccgact cgcccgcgtc ggtgacgctc
     4801 acgctccgcg ccacgccgtt ttcggagctg cgggatttcg atccggcgac cgactcgttg
     4861 tggatattca gggagggaga cccgctctcc cccgaagatg acgactggat cgcggcggcg
     4921 ttcacgaccc tcgagcccga tacgtgcgcg gacggagggg caggacggcg cgtgaccgcc
     4981 ttccttcgac tcgccgccgg ccaaacgatg gacgcggggc aggtcacagc gggcgccccg
     5041 gttctgggat tcgagaccgt gacctaccgg ttgtatcgct cctccgccga tcggcgctgg
     5101 tacgtcggga tggaaacgtc gtccgacctc cagccggtgc tcggcccggt caccgacgac
     5161 ggactggcct tcagctatct ggattcgacc ggcgccacgg cggcccaggc agcccgcgtc
     5221 agggcgatcg agtttcgcgt ccgtgcacgt accgtggagc cggtccgaac ccgggacggc
     5281 aggctcgatc atccgctgga cagcttgttc gcggtggtct tcctgcgcaa caacccgcgg
     5341 ttctgagcca tgccctgcgt cgccacggag aggcgcggga tcgcactgcc cctggtgctc
     5401 tgggccctgg tgatcgggtc ggccctgctg acggtcgcgg tcttcctgat cctccaggag
     5461 cagcgggcgg gtcaggccgg ccggcggctc atccatgcgt ttacgcgggc cgaggcgggg
     5521 ctcaccgaag tgctcggggc gtggacaccc ggatcgttga accgggcact tccgggggtc
     5581 ctcgattctc tcggtgtgac gggaagtgac ctcgcgattc gtcggctcaa tcagggcctg
     5641 tttctcctgg ccgtcagggt gcgcgacggc gcgaccgaag cacgactcgg ccgcctggtg
     5701 cgggttcgac ccgtccatct cgcggtccct gccgcgctca cctccggcgg accggtccga
     5761 ctgggcgggg aggtgaccgt cagcggagcg gatcagccgc ctgcaggctc ggaggattgc
     5821 ccgccagtcg acagcgccgt ggcgggcgtc gcggccccgg cggtgacgac gacggagtcc
     5881 acggtcatca cgggttcacc cccgaccgtc gagcgtcaac ccgccgactc cggtctcgcg
     5941 cccgaggatc tgctggtgtt ccgcgatctg gcgtctcagg cgacggtgag cctggcgggg
     6001 ggcgtgctgc agacggctcc tgtcactgtc gggacagtat gcagcctctc cgctgccgag
     6061 aactgggggg acccgtttgc cccgggcggt ccctgcggcg cctacatgcc gatcatctac
     6121 gtgacggggg acctcgatct gctctctggt cgggggcagg ggattctcct cgtggaaggc
     6181 cacttgagag tgcacggccc ctacagcttc gccggcctga tcatggcgcg tgggggactg
     6241 gacatcgcca cagcggatag cgcagtccat ctgaaagggg ccgttttcgc cggcagtgtc
     6301 ggcacgcaga cgacccctcc tgcagtactc tccatcacat actctaagtg tttggtatca
     6361 aaggctttgc tgagctcggg ccagctcgtt ccactgcgca ccaggagctg gaaagcgctc
     6421 ttctgaatca gctaactcta ttcctacaaa acagttgttt gattagcctc aagtaggttc
     6481 gaggcttgac aggataaggc tccggtctta ccgtagtttg cccttgtccc atcgtgttcc
     6541 ttcaataccc tggggagaca tggccctgtt tgggattggt gccaaaaaga cctcggtcgg
     6601 gctcgatatc ggttccggca tcgtcaagct ggtggtgatc gaccaccggg gagcggagcc
     6661 cgagctggtg cgcgtcgcga ccaccgaggt cgcggcggac gcgatcgtcg agggcgaaat
     6721 catggatccc gggatcgtgg ccgaggcgat tcgcggtctg ttttccacag ccggcgtcaa
     6781 acctaaaggg atcgtgacgg cggtcggcgg ccgggacgtc atcgtcaaga agatccaaat
     6841 ggaccgaatg cgcgaggccg aggcccgcga cgtgattcgc tgggaggccg agcagcacgt
     6901 gccattcgat atggccaacg tcgagctgga cttccagatt ctcgacccgg aagccgaggg
     6961 gctgcagatg aacgtgctcc tcgtggccgc caagcgtgag ctggtggaag gccgggtgag
     7021 cctgctgcgg gaagccgggt tggagcctgg aatcatcgac gtcgatgcgt tcgcgatcca
     7081 caacgcgttc gagctcaatt accccgaggc gatgcacggc gtggtgggtc tggtcaacat
     7141 cggccacgaa gtgaccaacg tgaacatcct ggacgacggg gtgccggtgc tgacgcggga
     7201 cctgtcggtg ggcacgcgcc gcttccggga agatctgcag cgggagaagg gtttgtcggc
     7261 cgaagactcg gagcgggtcg tgaccggcgc ggcgcagagc gcggatctct cgggctacgt
     7321 cgaggcgcgc ggtgaggaaa tcgccgtcgg ggtggagcgg gcggcggcgt tcctggcgac
     7381 ggcctcacgc tcggcggggg ggctggcacg ggtgttcacg agcggcggcg gcgcgcgggt
     7441 tccgggactg aacgaagccc tggcccagcg cctcaaggtg ccggtggagc ccgccaatcc
     7501 ggtgcagcgg ttgaaagtgc gcgacacggt ctacgagtcc gtgtcgctcg acgaggtcgc
     7561 accactcctg atgctctcgg ttggactggc gctgaggcgt gccgcatgat cgaaatcaat
     7621 ctgctgccgg ggcagaagcg gaaggcggcc ggaggcggcg ggctccggat gccggacttc
     7681 cggtcggtct tgaccaacgt gaaggacccg tggctgctgg gcgcgatcgc cgcgggcgtg
     7741 attctcatcg gcgggaatct ggcactgtac ctcgtgaaca ccgcgcggct cgcgcggctc
     7801 gacagcgtgc tgcgcggcgt gcaggcggag aagcgccgct atgacgcggt cgtcgcccag
     7861 aagcggcagg ccgagcgcgt gcgcgattcg ctggcggcgg agatggtgat cattcgcggg
     7921 atcgacggcg atcgctacat ctggccgcac ctgctggacg agatcaccaa ggcgctgccg
     7981 gcctacacct ggctcacggg cattcaggcg acctcgggtg tggcgccggc tgtggggctc
     8041 ccttccgggg cggccccggc ggccgacacc ggcgccgggc agggcgtacg cgtggggatc
     8101 gatgggcgga cggtggacat ccaggcctac accacgttcc tgcgccagtt ggcggcgtcg
     8161 ccctggctgg ccgacgtgat gccgacgaaa gcgacgaccg tgatcgaaca ggaccggccc
     8221 gtgactgaat tcaacatcac cgtgcggtac cgccaggcgg actctctgta tgtccgcacg
     8281 gtcccgctgg tgcagtcggt gaggtaagcc gtggcactcg gcattcccca ggactcgcgc
     8341 agccaggtga tgctgctgat catcgtcgcc tgcctggccg gtggctattt cttctggacc
     8401 aagatccaga gccccaagca ggcgtccatc accgcgacgg gacaggagat cgacagcctc
     8461 gaggcggtgg ttcgcaaagc gaaggcggat ctggcgggcg gcagcgtgga gagcatgcgc
     8521 cgctcggtcg agcgctaccg cgccgccctg ggtctgatgc gccggctcgt gcccgagcag
     8581 aatgaagtgc ccagcctgct ggaggacatc tccaaccggg cgaaggtgcg cggcatccag
     8641 ctgggcagct tccaaccact gagcccggag ccggggccac cgctggcgct cacctaccgg
     8701 gagcccggcg atacggggaa gaagaaggcc ggcgccccgg cgttcgacat ctatcgctac
     8761 cggctgcagg tgtacgggca ctacgaccag atcggggagt tcctggccga cgtgtcgagc
     8821 ctgccccgga tcatggttcc gcaggccctg gtgctgcggg cggcgagcca gcagacccag
     8881 aagctggtgg gagatacgct gggcgcgtta ctcgaggcga gcttcgacct gcggacgtac
     8941 gtgaagcggg ttcctccctc cacgtcgagt gccggggggg cgatcgatgc gcgtccgtag
     9001 cctgctcatc gccggctgct ggctggcggt cccggccgcc gggcagaccg cgcgcgccga
     9061 cagcgcccgc gactccctgg cgcgccccga caccgctccg ctcacgctgg tccgcgaagt
     9121 gttctcctat cagggagcgg gtcgcgatcc gttcatgtcg ctcctgaaat ccggggacgt
     9181 gcgtcccctg atcacggatc tcaagctcgt gacggtgatc tacgacgagc gctacgcggc
     9241 gcggagcgtc gtcgtgctgc gggacatcac cacctcgaag cggtaccggg cgcgggtcgg
     9301 cgacgtgatc ggccgcttgc gtgtcaccca gattcgtccg cgcgaagtcg tgttcacggt
     9361 ccaggaattc ggctttgagc ggcagcagac gctgtccctc gccaagcagg aggagaatcc
     9421 atgaagcgca gtatcgtaac gatcgcgtgc cttgccgttg cggcggcccc gcgccttccg
     9481 gccgacccga cacgggacgg cgaagtccgc gcgatctccg tggcgccggc gagcggacgg
     9541 gtggacgtgg tcatcggcct gcagggtgcg gtggaggtgc aggacttcac gctcgaagcg
     9601 ccggcccggc tggtcatcga cctgctcggc gcccggttga ccgcgcccgc cgccctgtac
     9661 gacgggcaga atcggggcgg cgtccggaac atccgctacg cgcagttccg cccgggtgtg
     9721 gtgcgggtgg tgatcgagct cgacgccctc gccgactacc agattcagcg gaccgacggc
     9781 caggtccgcg tggagatcgg caccgagcgc acggcgttcg gggcctggtc gagcagcaac
     9841 acgccgcgcg tcgccgcggc gccggccgcg tcggagcccg c
//
LOCUS       DBNGZW010000723         3542 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1073320, whole genome shotgun sequence.
ACCESSION   DBNGZW010000723 DBNGZW010000000
VERSION     DBNGZW010000723.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1073320"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..902
                     /locus_tag="AC16JC_17030"
     CDS             228..902
                     /locus_tag="AC16JC_17030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR5166458.1"
                     /translation="MQILIVEDDRDLRRFLSKAFREEGYGVVEAETGDGALDRALGAA
                     CDCIVLDVMLPGRDGFDVVKELRGRNVTTPVLMLTARDEVESRVRGLEGGADDYLTKP
                     FDLSELIARVNALIRRADLRHKDATLRVGVITLDPLTRSVTQNERRVDLSPREFSLLE
                     FLMRNAGRTVSRSRIAEAVWNYKFDPETNVVDVYINYLRKKLTFAGARPPIRTVRGAG
                     YRLEAS"
     gene            899..2341
                     /locus_tag="AC16JC_17035"
     CDS             899..2341
                     /locus_tag="AC16JC_17035"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5166459.1"
                     /translation="MSERRLGAVLTTRFSRLTWSALAGYALLVVVVFALASEAVLRRS
                     LEHTADVTRSLIGAYGDSAGGPGSVMPTALADQLLDMGGRVVITRTTNADRMMQVYFL
                     SPGMPAKRLEGIPASASPDDVRRILLAAIAQRARWRYRALHRASGAFDIYLVASREPF
                     AVALLGLVVAAALLLPVAAFGATRGARTAVAEALRPLERISGETRAIGPEDLGKRIDS
                     PTGQTEITELAESINRMLGRVDRAHRALESFTADASHELRTPLTHLRAQVQWASAQHR
                     TPSPVRESLAAMETELERTTKMVDELLLIARGESRRLALARAPFDLTAVVEDVQEITE
                     AMAVGKGLDVGCTEWAPAPVAALGDANRTRQILLNLASNAVRYTARGCVTFFIREDGD
                     RVGVSVQDTGSGIAAEHLPHVFDRFFRVEQSRNRALGGTGLGLAIARLLAELQGGDIA
                     VESVLGHGSTFTLWLPRAVAVESDSLLRSG"
     gene            complement(2327..>3542)
                     /locus_tag="AC16JC_17040"
     CDS             complement(2327..>3542)
                     /locus_tag="AC16JC_17040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166460.1"
                     /translation="ALGTTGLAWIDAASRSAANTNAELAGRNVAQLVAEPDATPAQLV
                     YLAAHEAFHVFERGSRREGKRFGGGENAFLVTSYPVFDRDNEAGFALEARVLAAALAA
                     PGDSAARAFAWEFLALRDARQRALGSDGAEFERMAELNEGLAEYAGLRAVQVGPLDPA
                     WRDGAARVAARIAGRLDSVTTQSRNSLRLRFYATGPALGRLLDRLAGERWKPELVRED
                     RTLQEELAAVTGYGTREARLIAAARARFDWLALTKRTDTTIARLQALRQAQRDSILAA
                     PGIELVIDASAVGGMGMCGIDPQNLLQAGQGVLLHRRWVRPCVGKALDAEFTTPVVQD
                     QGAGTVLAVIGDETTLRWTAGGAPLALPDGARHGDVRDLRLEAPLVTVQAAHVAVERS
                     GRTVTVRLLPAS"
BASE COUNT          492 a         1243 c         1266 g          541 t
ORIGIN      
        1 gcatccgaat gcggcggtcg ctgcgggatc gccgtccccg gacggcgatg gtggtcgcga
       61 gcgctagcac ttcctcttca agtgttgatg tccggtgcca ccaccaggtg gcatgcgtca
      121 gcaacgcgtt aacgcaacgc agcgcatcgg tcgatgcgtt gttagaggac tgtcattctt
      181 ccattgcccg acgccccgcc cggcgtgccc ctcgtagctt tcgcgccatg cagattctga
      241 tcgtcgaaga cgaccgcgac ctgcggcggt ttctgagcaa ggccttccgc gaggagggct
      301 acggcgtcgt cgaggccgag acgggcgacg gggcgctgga tcgcgcgctc ggcgccgcct
      361 gcgactgcat cgtgctcgac gtcatgctcc cggggcggga cggattcgac gtggtgaagg
      421 agctgcgcgg gcggaacgtg acgactccgg tgttgatgct gaccgcgcgc gacgaggtgg
      481 agtcccgcgt gcgtggcctg gagggcgggg cggacgacta tctcacgaag ccgttcgacc
      541 tgtcggagct catcgcgcgg gtcaacgccc tgatccggcg cgccgatctg cgtcacaagg
      601 atgcgaccct gagggtgggg gtaatcaccc tcgacccgct gacccgcagc gtcacgcaga
      661 acgagcgccg ggtggacctg agcccgcgcg agttttcgct gctcgaattc ctgatgcgca
      721 acgccggccg gacggtgtcg cgctcgcgca tcgccgaagc cgtatggaac tacaagttcg
      781 atcccgagac caacgtcgtg gacgtctaca tcaactacct gcgcaagaag cttaccttcg
      841 ccggcgcgcg tcccccgatt cgcaccgttc gtggggcggg gtatcggctg gaggcgtcgt
      901 gagcgagcga cgtctcggcg ccgtcctcac gacgcggttc agccgcctca cctggtccgc
      961 gctggcgggg tacgcgctcc tcgtggtcgt cgtcttcgcg ctggcgtccg aggcggtgct
     1021 gcgccgttcg ctcgagcata ccgcagacgt cacccggtcc ctgatcggcg cctacggcga
     1081 ttccgcgggc gggccgggta gcgtcatgcc gacggcgctc gcggaccagc tgctcgacat
     1141 gggcggccgg gtcgtgatca cgcggaccac gaatgcggac cgcatgatgc aggtctactt
     1201 cctgtcgccc gggatgccgg cgaagcggct ggaggggata cccgcgtcgg cctcccctga
     1261 cgacgtgcgc cgaattctgc tggccgcgat cgcccagcgg gcgcgctggc gctatcgggc
     1321 gctgcaccgg gcgagcggcg ccttcgacat ctacttggtg gccagccgtg agccctttgc
     1381 ggtcgccctg ctcgggctgg tggtcgccgc cgcgctgctg ttgccggtgg cggcgttcgg
     1441 cgccacccgc ggcgcgcgca cggccgtggc ggaggcgttg cgtccgctgg agcgcatctc
     1501 cggggagacg cgggcgatcg gccccgagga tctgggcaag cggatcgaca gcccgaccgg
     1561 tcagacggag atcaccgagc tcgccgagag catcaaccgg atgctcgggc gggtggaccg
     1621 cgcgcaccgg gcgctcgagt ccttcacggc ggacgcgagc cacgagctgc ggacgccgct
     1681 gacgcacctg cgcgcgcagg tgcagtgggc ttcggcgcag caccgcaccc cgtcgccggt
     1741 ccgggaatcg ctcgccgcca tggaaaccga gctcgagcgg acgaccaaga tggtggatga
     1801 gctgttgctg atcgcccggg gggagagccg gcggctggcg ctggcccgcg ccccgttcga
     1861 tctgaccgcg gtggtggagg acgtgcagga gatcaccgag gcgatggctg tgggcaaggg
     1921 cctcgacgtc gggtgtaccg agtgggctcc ggcccccgtc gcggcgctgg gggacgcgaa
     1981 tcggacgcgg cagatcctgc tgaacctggc ctcgaacgcc gtgcgctaca ccgcgcgcgg
     2041 ctgcgtgacg ttcttcatcc gggaagacgg cgacagggtc ggcgtgagcg tgcaggatac
     2101 gggttcagga atagccgcgg agcacctgcc ccatgtgttc gaccgtttct tccgcgtcga
     2161 gcagtcgcgc aaccgcgcac tgggcggcac gggactcggc ctcgcgatcg cccgtctcct
     2221 ggccgagctg cagggaggcg acatcgcagt cgagagcgtg ctcggccacg ggagcacgtt
     2281 tacgctctgg ctgccgcgcg cggtcgccgt ggaatccgac tccctgctac gaagcgggta
     2341 gcagccgcac cgtcaccgtt cgtccgctcc gttccacggc gacgtgggcc gcctggaccg
     2401 tcacgagcgg cgcttcgagt cgcagatcac gcacatcgcc gtgtcgcgcg ccgtccggca
     2461 gggcgagcgg cgccccgccc gcggtccagc gcagcgtcgt ctcgtcgccg atcacggcga
     2521 gaaccgtgcc ggccccctgg tcctgcacca cgggcgtcgt gaactcggcg tccagtgcct
     2581 tgcccacgca gggccgtacc cagcgccggt gcaacagcac gccctgtccc gcctggagca
     2641 ggttctgcgg gtcgatgccg cacatgccca tgccgccgac cgcgctcgcg tcgatcacca
     2701 gctcgatgcc cggcgcggcc aggatcgaat cgcgctgagc ctgccgcagc gcctggagcc
     2761 gcgcgatcgt ggtatccgtg cgtttcgtca aagcgagcca gtcgaaccgt gcccgcgccg
     2821 ccgcgatgag ccgggcttcg cgggtcccgt acccggtcac cgcggcgagc tcttcctgta
     2881 gcgtacggtc ctcgcggacc agctccggtt tccaccgctc gcccgcgagg cggtcgagca
     2941 acctccccag ggcgggaccg gtcgcgtaga agcgcagccg gagcgagttg cgcgattgcg
     3001 tcgtgacgct gtccaatcgg cccgcgatcc gcgcggccac tcgcgctgct ccgtcacgcc
     3061 acgcgggatc gagcggtccc acctgaacgg cgcggaggcc ggcgtactcc gcgagtccct
     3121 cgttcagctc cgccatgcgc tcgaactccg ctccatcgct gccgagggcg cgctggcgcg
     3181 cgtcgcgcag cgccagaaac tcccaggcga acgcgcgggc ggcggagtcg ccgggggccg
     3241 ccagcgccgc cgccagcacg cgcgcttcga gcgcgaagcc cgcctcgttg tcgcgatcga
     3301 agacgggata gctcgtcacg aggaaagcgt tctcgccgcc gccgaaccgc ttgccctcac
     3361 gacggctgcc gcgctcgaac acgtggaagg cttcgtgggc ggcgaggtag acgagctgtg
     3421 ccggagtcgc gtccggctcc gccacgagct gcgccacgtt acggcccgcc agctcggcgt
     3481 tggtgttcgc cgcggaacgg gaggccgcgt cgatccaggc gagtccggtg gtacccagcg
     3541 cc
//
LOCUS       DBNGZW010000724         2518 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1011906, whole genome shotgun sequence.
ACCESSION   DBNGZW010000724 DBNGZW010000000
VERSION     DBNGZW010000724.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1011906"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2518
                     /locus_tag="AC16JC_17045"
     CDS             <1..>2518
                     /locus_tag="AC16JC_17045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166461.1"
                     /translation="PGFWKGIEVQRNLALTTWRRALIEWAGGTRGPFGGVAAEACVLF
                     VNRSGAQVDLDSVRIRQCTHAGLHHFGGLSHVHRSQIDTVSGSAIHADFDARLEVDST
                     VLRGATNSGLEIPSPAVRLLPSSGNAVFGSGILAISIRSAQLPGMLRQDTIGGNAANI
                     IEVVAGAPVDTSFTLFRQPRGLVFGNDYDYRISGLLTVSGSLGPQVTLDSNLVVRFLS
                     QSGLVIGDSAGAGTGLVRSSGTVPTNAPRLTSIDIPGSPGEWLGVEVGRLAAADTLRN
                     VRIEFAGDSLPGRTVHRAGLWVRNPVPVPLVLEGVDLSANGSSGAAANSGGLVATANT
                     GGLDVRNSTVAGNPGFGLVVPDSRFRVTGNQVTGNGIGIGAFVQGGSQITTADSIAGN
                     TIAANGYAVALTAAALRAFYDTVVPVNTTDTLLLESSLLTADARLPRVPGFIWHAAGA
                     MTIDSNATFTITPGNTVTFDTLAALLGGTVPAFVKDLPSAPAAITIGGVRPGALAAPG
                     TAAAPIVLTSSVPGRGWAGIQWAFPQGTGAITNNVFTYVTVERAGYYAPCFNDCSPIP
                     FSALRITDSTDVVLSFDHLIVRGSGAFGLDFERGGTGTLTVSNSQFYNNNGGIRALSG
                     HGNQLTIIGSDLYEYRGTVIEGTYGGVDSVNADGNWWGDVDGPYPGFSYADSLGRGSL
                     GGTAVRVATLVGAPFFPLGPAARMIAARDTFLVADTVLSRVGQSDSLRVRVLDAEGRG
                     VVGVPVSWTTTSGSASPGSGSSDIGGRVATAWQTGSLAGAQRTTASGVGPPVVFPLDL
                     LAGNTVTTTVDWVMEPTLTVADSVHRHAGAGTDTVFYSSSN"
BASE COUNT          362 a          865 c          875 g          416 t
ORIGIN      
        1 gccgggtttc tggaagggaa tcgaggtgca gcgcaatctg gcactcacca cctggcggcg
       61 cgccctcatc gagtgggccg gcggcacccg cgggccgttc ggcggcgtcg ccgccgaggc
      121 gtgcgtcctg ttcgtgaacc gttccggcgc ccaggtcgat ttggacagcg tgcgcatccg
      181 ccagtgcacc cacgccggcc tccatcactt cggcggtctg tcgcacgtgc accgctcgca
      241 gatcgatacg gtctcgggct cggcgattca cgcggacttc gacgcgcgcc tcgaggtcga
      301 ttccaccgtc cttcgtggag cgacgaacag cggcctcgag atcccgagcc cggccgtcag
      361 actgctgccg tcgtccggca atgccgtctt tggcagcggc atcctcgcga tctcgatccg
      421 gagcgcgcag ctccccggga tgctgcgaca ggatacgatc ggcggcaatg cggcgaacat
      481 catcgaggtg gtcgccggtg cgccggtcga cacgtccttc acgctgttcc ggcagccgcg
      541 cgggctggtg ttcggcaacg attacgatta ccggatcagc ggtctcctga cggtgagcgg
      601 atcgctcggg ccgcaggtca cgctcgactc gaatctcgtg gtgcggttcc tgagccagtc
      661 gggactcgtc atcggcgatt cggccggggc ggggacgggc ctcgtccgct cgtcagggac
      721 cgtgccgacg aacgcgccca ggctgacgtc catcgatatc cccggatcgc ccggcgagtg
      781 gctcggtgtg gaggtcggcc ggctggcggc ggcggacacg ctgcgcaacg tccgcatcga
      841 attcgcgggc gactccctcc cgggccggac cgtgcatcgg gccggcttgt gggtgcgaaa
      901 cccggtccca gtcccactcg tgctcgaggg cgtggacctc tccgcgaacg ggtcgagcgg
      961 tgcggcggca aattccggtg gcctcgtcgc gaccgcgaac acgggcggcc tggacgtacg
     1021 gaacagcacc gtcgccggca accccggctt cgggctcgtc gtcccggata gcaggttccg
     1081 cgtcacgggc aaccaggtca ccggcaacgg tatcggcatc ggggcgttcg tccagggcgg
     1141 gtctcagatt acgaccgccg actcgatcgc cgggaacacg atcgccgcga acggttatgc
     1201 ggtcgctctc accgcagccg ccctgcgggc cttctacgac acggtggtgc ccgtcaacac
     1261 caccgacacg ttgctgctcg aaagcagcct cctgaccgcc gacgcgcgcc tgccgagggt
     1321 cccgggcttc atctggcacg ccgctggcgc gatgacgatc gactccaacg cgacgttcac
     1381 gatcacgccc gggaacaccg tcaccttcga tacgctggcg gcgctcctgg gtggcacggt
     1441 gccggcattc gtcaaggacc ttccctctgc tccggcggcc atcacgattg gtggggtgcg
     1501 gccgggcgcg ctggccgctc ccggcacggc ggctgccccg atcgtcttga cgagcagtgt
     1561 tcctggtcgg gggtgggcgg ggattcaatg ggccttcccg cagggcacgg gcgccatcac
     1621 gaacaacgtc ttcacctacg tcaccgtcga gcgcgccggc tattacgcgc cgtgtttcaa
     1681 cgactgctcg ccgatcccgt tctccgcgtt gcgcatcacc gattcgacgg acgtcgtcct
     1741 ttcgttcgat catctcatcg ttcggggcag cggagcgttc gggctggact tcgagcgcgg
     1801 ggggaccggg acgctcacgg tgtcgaacag ccagttctac aacaacaacg gcggcatccg
     1861 cgccctcagc ggacacggca atcagctcac catcatcggt tccgacctgt acgagtaccg
     1921 aggcaccgtg atcgagggga cgtacggcgg agtggattcg gtcaacgccg acgggaactg
     1981 gtggggcgac gtggatgggc cgtatcccgg gttcagctac gccgacagtc tcggccgcgg
     2041 gagtctcggc gggacggccg tgcgggtggc gacactggtc ggcgccccgt tcttcccgct
     2101 cggtcccgcc gcacgaatga tcgcggcgcg ggacaccttc ctggtcgccg acacggtgct
     2161 gagccgggtc gggcagtcgg actcgttgcg ggtgcgcgtg ctcgacgccg aaggccgcgg
     2221 tgtggtgggc gtgcccgtga gctggaccac aacgtcggga tcggcgagcc cgggttcggg
     2281 gtcctccgac atcggtggtc gggtggccac cgcgtggcag acgggcagcc tcgcgggcgc
     2341 ccagcgcact acggcgagcg gcgtcgggcc gcccgtggtg ttcccgctcg acctgctggc
     2401 gggcaacacg gtgaccacga ccgtggactg ggtcatggaa cccacgttga cggtagcgga
     2461 ctccgtccac cggcacgccg gtgcagggac cgacaccgtc ttctattcca gcagcaac
//
LOCUS       DBNGZW010000725         7368 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1073339, whole genome shotgun sequence.
ACCESSION   DBNGZW010000725 DBNGZW010000000
VERSION     DBNGZW010000725.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7368)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7368)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7368
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1073339"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1108)
                     /locus_tag="AC16JC_17050"
     CDS             complement(<1..1108)
                     /locus_tag="AC16JC_17050"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR5166462.1"
                     /translation="MTQGNQTPAPAWMFTSDANTLVGVADQAPRLEAEVRNAAVAQLL
                     AGSPLVEWLHLPLVLLVAALVWNSLPIELTIGWVTAVTAAAGLRAWWRVRMRRRAPSA
                     QEAVRGVRLTVSGVGLAWGFGAAAAIPALQLHEGVLALVVIAGLVAGATSTLVGDRRS
                     FRYLLITMLAPLPVGILIKGHARPEIIAIAMILIFAWGMDRVHGSAHRTFVERIRATV
                     LLELSGEQLTRQHGYLTTLLASAPVAIAIVDMGGRVRGVNPRFRALFGYSAEEMMDRE
                     LDDLLVPAAERASAARLTARVRDGETVATEVERRGKDGRLIAVRITAARLSGSASGDI
                     VVLYEDVTERRRAEAAVREARDAAEHAARARSAFL"
     gene            1199..3484
                     /locus_tag="AC16JC_17055"
     CDS             1199..3484
                     /locus_tag="AC16JC_17055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HIR5166463.1"
                     /translation="MSAPPSPFEDLSVVAVRASPCGILAVDASGVIVFVNPEIERIFG
                     YRAEDLTGQPLNRLIPPRFHTAHTGYQVRFLRAGEARHLGTGRELYGLHRDGREIPVE
                     IALTNVERPEGTITLASIIDITQRRQAEVRSREREAYYRTILENVVDAVCVTDAEGRY
                     LEVNQQACRLTGYTREELLQRSVSDTYLPQEREIAAARVADAAAGGQIRMERPLLRKD
                     GSVIRVEVGAVRLPDGRLLATLHDVTERNRAEEQLRESEQRFRELAEHVQEVFFVVDP
                     VSWRALYVSPAYEALAGRARAAAYAGPYSWAEGVHPDDRERVKATARNAAQTGEVTAV
                     VFRVVRPDQSIRWVRARGTAVRNAAGRMVRLVGIAEDVTDLRRTEEQFLQAQKMEAVG
                     RLAGGVAHDFNNLLTVITSYTQLQLDDLSYSDPRRADLEEILHAAREAANLTRQLLAF
                     SRRQMLELKVVNVNEVVAGIQKMLKRVIGEDIDLSVSLAPDLGGTRADAGQLEQVIMN
                     LAVNARDAMPGGGRLTIETANVKLDAPYASTHTPVTPGRYVLLAVSDTGVGMDESTKA
                     RIFEPFFTTKEMGKGTGLGLATVYGIVKQCDGFIWVYSEPGHGATFKIYLPSVAGQAE
                     PPTPAAGADPVAGGTETVMVVEDDPAVRGAVVTLLERWGYTVVQAERPTDALELARAA
                     PRPPDLLITDVIMPGMNGREVAREFEARYPRIRTLYLSGYTDEAIVRHGVLEPGVSFL
                     QKPFTPEGLARKVRQTLERGP"
     gene            complement(3525..5456)
                     /locus_tag="AC16JC_17060"
     CDS             complement(3525..5456)
                     /locus_tag="AC16JC_17060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5166464.1"
                     /translation="MGTPLRVLIVEDNPDDAALLLRQIHRAGYDPVAERVDTERAMQA
                     ALATGEWDVVLSDASLPQFSAEAALRVLHATGIDLPFIVVSGSIGEAAAVALMKAGAH
                     DYLFKENLARLGAAVDRELQDARERRDRQRAELDLRHSERRLAEAERLAHIGSWERDL
                     RTEATTWSAEFCRILGTAPSEMGETLEALLHRVHPEDRERVKAAIERVTQPPFLFDFE
                     FRLVRPDGALRQARARGLATMDGAGIPVRLLGTLQDVTEQRSLEAQFRQAQKLEAVGR
                     LAGGVAHDFNNILTVIMSYAEFVLDALPPGETIRQDVEQIRTAGASAAQLTRQLLAFS
                     RQQVLAPKVLNLNAVVTDAAKMMKRLLGDDIRLVTTLSPEVGMVKADSGQIEQVVMNL
                     AVNARDAMPEGGQLTIETANVEMDESYLREHALARKGRYVMLAVTDTGVGMDERTKAH
                     MFEPFFTTKEAGKGTGLGLATVYGIVKQSGGFVWVYSEAGRGTTFKIYLPRTDESSDP
                     LDAGFRTGSLRGTETILVVDDAPAVRTAVRTVLERHGYVVVEAPNGEAALQLATKHRG
                     PLDLLVTDVVMPGLSGRDLAGNLSSQRPGMRVLYMSGYTDDTIVRHGVLEPGIRFIQK
                     PFSPDALAQKVRETLDAPR"
     gene            complement(5457..5909)
                     /locus_tag="AC16JC_17065"
     CDS             complement(5457..5909)
                     /locus_tag="AC16JC_17065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009632724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5166465.1"
                     /translation="MTTTRNRMILLVEDSPDDEALTLRAFAKSNIRNAVVVVHDGVEA
                     LDFLFCTGTYAGRDPEDRPEVILLDLKLPKLDGLEVLGRLRADERTKLLPVVILTSSR
                     QDQDLVAGYRLGANSYIKKPVDFEQFVEAVRNLGLYWLVLNEPPPDRV"
     gene            complement(5906..>7368)
                     /locus_tag="AC16JC_17070"
     CDS             complement(5906..>7368)
                     /locus_tag="AC16JC_17070"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015154448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5166466.1"
                     /translation="ERAQLDSLRQSISLRIGALRTLLARPHGARAPARDGEASIGATS
                     IADQVRRLATRMEDRSERLALRRVAILDGRARLARLSVFGGTALAFLVVIAASVLVGR
                     ELAERRRAEDAVQESERRLAQLLDAMPIGVFVLDAVGGSSYANQVAQSILGKGIVVGA
                     AGADLPEIYQAYVPGTDDTYPADRQPIMEALRGRKPPPAVMEIRRPDRMVPIEVLAAP
                     VFDRQGTVVQAVAAFNDISERERSRAEIERLNAELSRKLAELMAVNQELEAFSYSVSH
                     DLRAPLRSIDGFSQALLEDCAALLDEHGKDYLRRVRAATQHLAELIEDLLGLARVTRA
                     ELRRESVDLTAMARSVAGELSEREPGRPIDFLCAEGLAVDADPRLLRVLLTNLLGNAW
                     KFTTRQPRARIEFGRTVDGGRPAYFVRDNGVGFDMTYAEKLFGTFQRLHSAVEFPGTG
                     VGLATVQRIVHRHGGRVWAHAVVGQGASFYFTLEAR"
BASE COUNT         1165 a         2599 c         2483 g         1121 t
ORIGIN      
        1 ccaagaacgc gctccgcgcc cgcgcggcgt gttccgccgc atcacgcgcc tcccgcaccg
       61 ccgcctccgc acggcgccgc tcggtgacgt cctcgtacaa cacgacgata tcgccgctcg
      121 ccgagccgct gagccgcgcg gccgtaatgc gcacggcgat caaccggccg tccttgcctc
      181 gccgctcgac ctccgtcgcc accgtttcgc cgtcgcgcac ccgcgccgtc aggcgagcgg
      241 cggacgcgcg ctcggccgcc gggacgagca ggtcgtccag ctcccggtcc atcatctcct
      301 cggctgagta tccgaagagc gctcggaatc gcgggttcac gccccgcacc cgtcccccca
      361 tgtccacgat cgcgatcgcc acgggtgcgc tcgccagcag cgtggtgagg tacccgtgct
      421 ggcgggtcaa ctgctcgccc gacaactcca gcagcacggt ggcgcggatt cgctccacga
      481 aggtgcgatg ggcgctcccg tggacccggt ccattcccca ggcgaagatc aggatcatgg
      541 cgatcgcgat gatctccggc cgcgcatggc ccttgatgag gatccccacc gggagcgggg
      601 ccagcatcgt gatcagcagg tagcggaagc tgcgccgatc accgaccagc gtgctggtcg
      661 ccccggcgac cagtcccgcg atgaccacca gcgcgagcac cccctcgtga agctggagtg
      721 ccggaatcgc cgcggcggcg ccgaagcccc aggcgaggcc gacgccggag acggtgaggc
      781 ggaccccacg cacggcctcc tgggccgatg gcgcccgccg ccgcatccgc acacgccacc
      841 aggcccgcag ccccgccgcc gcggtgaccg ccgtcaccca gccgatcgtg agctcgatcg
      901 gcaacgagtt ccacacgagg gcggcgacga ggagcacgag tgggagatgc agccactcga
      961 cgaggggcga tccggcgagc aactgcgcga cggccgcgtt ccgtacctcc gcctcgaggc
     1021 gcggcgcctg gtccgcaact cccacgagcg tgttcgcgtc ggaggtgaac atccaggcgg
     1081 gcgcaggtgt ctgattcccc tgagtcatgg aggctatttg acaccctcca cgtccgggag
     1141 caagtggcgt tccctgcttg accccgctga ccccgagtcg cactttatcg ttgagacgat
     1201 gtcagcccca ccatcgccgt tcgaagacct gtcggtcgtc gccgtacgcg cctcaccctg
     1261 cggcatcctc gcggtcgacg cgtcaggcgt gatcgtcttc gtcaatccgg agatcgagcg
     1321 catcttcggc taccgcgctg aagacttgac cggccagccg ctcaaccggc tcatcccccc
     1381 ccggttccac acggcacaca ccggatacca ggtgcgattc ctccgggccg gcgaagcccg
     1441 gcacctcggc accggccggg agctgtacgg gttgcaccgg gacggtcgcg agatcccggt
     1501 cgagatcgcc ctcacgaacg tggagcgccc cgagggcacg ataaccctcg catcgatcat
     1561 cgacattacg cagcgccgac aggcggaagt ccgctcgcgc gagcgtgagg cttactaccg
     1621 cacgatcctg gagaacgtcg tcgacgccgt gtgcgtgacg gacgcggagg gacggtacct
     1681 cgaagtcaac caacaggcct gccgactgac cgggtatacc cgcgaggagc tgctgcagcg
     1741 gagcgtgagc gacacctacc ttccgcaaga gcgggagatc gcggcggcac gagtggcgga
     1801 cgccgccgcg ggcgggcaaa tccgcatgga acggccgctg ctgcgcaagg acggttccgt
     1861 catccgggtc gaggtcggtg cggtgcggct gcccgacggc cggctgctgg cgacgctgca
     1921 cgacgtgacc gagcgcaatc gagccgagga gcagttgcgc gaaagcgagc agcgcttccg
     1981 cgagctggcc gagcacgtcc aagaggtgtt cttcgtggtg gatccggtat cgtggcgcgc
     2041 gctctacgtc agtcccgcct acgaagccct ggccggacgc gcgcgcgcgg cggcctatgc
     2101 cggaccgtac tcgtgggccg agggggtgca tcccgacgac cgggaacggg tgaaggccac
     2161 cgcgcggaac gcggcacaga cgggtgaggt cacggcggtg gtgttccggg tcgtgcgccc
     2221 ggaccagtcc atccgctggg tgcgcgcgcg ggggaccgcg gtacgcaacg ccgcggggcg
     2281 catggttcga ctcgtcggga tcgccgaaga cgtcacggac ctacggcgga ccgaggagca
     2341 gttcctgcag gcgcaaaaga tggaggcggt gggccgcctg gccgggggcg tcgcgcacga
     2401 tttcaacaat ctcctcacgg taatcacgtc gtacacgcag ctgcagctcg acgatctctc
     2461 ctactccgat ccgcggcgtg ccgatctcga ggagatcctg cacgctgcac gggaggcggc
     2521 caacctcacc cgccagctcc tcgccttcag ccggcgacag atgctcgagc tgaaggtggt
     2581 gaacgtgaac gaagtggtcg cggggatcca gaagatgctc aagcgggtca tcggcgagga
     2641 tatcgacctc agcgtctcgc tggcccccga cctgggcggc acacgcgccg acgccggcca
     2701 gctggagcag gtcatcatga acctggccgt caatgcacgc gacgcgatgc cgggcggcgg
     2761 acggctcacg atcgagacgg ccaatgtcaa gctggatgcg ccctatgcga gcacccatac
     2821 gccggttaca ccgggccgct acgtgctcct ggcggtgtcg gacacgggcg tggggatgga
     2881 tgaatccacg aaggcccgca tcttcgagcc gttcttcacc accaaggaga tgggcaaggg
     2941 caccggactc ggcctggcta ccgtatacgg catcgtcaaa caatgcgacg ggttcatctg
     3001 ggtctactcg gagccggggc acggcgcgac gttcaagatc tatctcccca gcgtcgccgg
     3061 gcaggccgag ccgccgacgc cggcggccgg cgctgacccg gtcgcaggag gcaccgaaac
     3121 cgtcatggtg gtggaggatg acccggcggt acggggagcc gtggtcaccc tgctcgagcg
     3181 ctggggctac accgtggttc aagcggagcg ccccacggat gcgcttgagc tcgcccgcgc
     3241 cgcaccccga ccgcccgatc tcttgatcac cgacgtgatc atgccgggaa tgaacgggcg
     3301 ggaggtggcg cgcgagttcg aggcgcgcta cccccggatc aggacgctgt atctctccgg
     3361 gtataccgac gaggcgatcg tgcgtcacgg cgtgctggaa cccggcgtgt ccttcctgca
     3421 gaagccgttc acccccgaag gtctggcgcg gaaggtacgg cagacgctgg agcgcggccc
     3481 gtagctggtc tcatggcgcc tctgccgcga gcggcggggg cgtgtcagcg cggcgcgtcg
     3541 agcgtttcgc gcaccttctg cgccagagcg tccggggaga agggcttctg aatgaagcga
     3601 atgcccggct ccagcacccc gtgccggaca atcgtgtcgt ccgtgtagcc cgacatgtac
     3661 agcaccctca tgccgggccg ttgtgacgag agattgccgg ccaggtcccg cccgctcaat
     3721 cccggcatga cgacgtccgt gacgagcaga tcgagggggc cgcgatgttt tgtcgcgagc
     3781 tgtaacgcgg cctctccatt cggggcttcc accaccacgt agccgtggcg ctcgagcacg
     3841 gtgcgcaccg cagtgcggac cgccggcgcg tcatccacca cgaggatcgt ctcggtaccc
     3901 cgaagcgagc cggtgcggaa gccggcgtcg agggggtcgg acgactcgtc cgtgcggggg
     3961 aggtagatct tgaacgtcgt cccacgacct gcttcgctgt agacccagac gaatcccccg
     4021 ctctgcttga cgatgccgta cacggtggcg aggccaagcc ccgtgccctt gcccgcctcc
     4081 ttggtggtga agaagggctc gaacatgtgg gctttggtcc gctcgtccat gccgacgccg
     4141 gtgtcggtca cggcgagcat gacgtagcgg ccctttctcg cgagcgcatg ctcccgcagg
     4201 tacgactcgt ccatttcgac gttcgccgtt tcgatggtca gttgcccccc ctccggcatc
     4261 gcgtcgcggg cgttgaccgc gaggttcatg accacctgct cgatctggcc ggagtcggct
     4321 ttcaccatac cgacctcggg tgagagcgtg gtgacgagcc ggatatcgtc gcccaacagt
     4381 cgcttcatca tcttggccgc atcggtcacc accgcgttga ggttcagaac cttgggggcg
     4441 aggacctgct ggcgactgaa ggcgagcagc tggcgggtca gctgggccgc gctcgcgcca
     4501 gcggtccgga tctgctcgac gtcctggcgg atggtctcac cggggggaag ggcgtcgagc
     4561 acgaactcgg cgtagctcat gatgacggtg aggatgttgt tgaaatcgtg ggcgacgccg
     4621 cccgccaggc gccccaccgc ttccagcttc tgcgcctgac ggaattgcgc ctcgaggctc
     4681 cgctgctcgg tcacgtcctg cagggtgccg agcagccgga ccgggatccc ggcgccgtcc
     4741 atcgtggcca gaccgcgcgc gcgggcctgc cgcagggcac catcggggcg tacaagccgg
     4801 aactcgaaat cgaacaggaa gggcggctgg gtcacgcgtt cgatcgcggc tttcacccgc
     4861 tcacggtcct cgggatggac gcggtgcagg agggcctcga gcgtctctcc catctcgctg
     4921 ggcgccgttc ccaggatccg gcagaactcg gcggaccagg tcgtggcctc ggtccggaga
     4981 tcgcgctccc agctgccgat gtgcgcgagc cgctcggctt cggcgagccg gcgctcgctg
     5041 tggcgcaggt ccagctccgc gcgctggcgg tcccggcgtt cgcgggcatc ctgcagctcc
     5101 cggtccacgg ccgccccgag ccgggcgagg ttctccttga agagataatc gtgggctccg
     5161 gccttcatga gggcgacggc cgccgcctcg ccgatcgaac cggagacgac gatgaagggc
     5221 agatcgatcc cggtggcgtg cagcacccgc agggccgcct ccgcgctgaa ctgcggcagc
     5281 gacgcgtccg acaagacgac gtcccactct cccgtcgcca gggccgcctg catcgccctt
     5341 tccgtgtcga cgcgctcggc gaccgggtca taccccgcgc ggtggatctg ccgcaggagc
     5401 aacgcggcgt cgtcggggtt gtcctcgacg atcaggacgc gcagcggcgt gcccacctac
     5461 acccggtccg gtggaggttc gttcaggacc aaccagtaaa gtccgagatt ccggaccgcc
     5521 tcgacgaact gctcgaagtc caccggcttc ttgatgtagc tgttggcccc cagccggtag
     5581 cccgcgacca ggtcctggtc ctgccgggag gacgtcagaa tcacgaccgg gagcagcttc
     5641 gtccgctcgt cggccctgag acgcccgagc acttcgaggc cgtcgagctt gggcagcttc
     5701 aggtcgagca ggatcacctc cgggcgatct tccggatcgc ggccggcgta cgtcccggtg
     5761 caaaacagga aatccagcgc ctcgactccg tcgtgcacga cgaccacggc gttgcggatg
     5821 ttgctcttgg cgaacgctcg cagcgtcagc gcctcgtcgt ccggactatc ctccaccagg
     5881 aggatcatgc ggttgcgcgt cgtcgtcatc gggcctcgag ggtgaagtag aagctcgcgc
     5941 cctggccgac caccgcgtgc gcccacaccc gtcctccgtg gcgatgcacg atgcgctgga
     6001 ccgtcgccaa cccgacgccc gttccaggga actccaccgc gctgtggagc cgctggaacg
     6061 tgccaaacag cttttccgca taggtcatgt cgaaccccac cccgttgtcc cgaacgaaat
     6121 acgccggccg gcctccgtcc accgtcctgc cgaattcgat cctcgcacgt ggctgtcggg
     6181 tcgtgaactt ccacgcattg cccaggaggt tcgtcagcag gactcggagc aggcgagggt
     6241 ccgcgtccac ggcgagaccc tcggcacaga ggaagtcgat ggggcgcccg ggctcccgct
     6301 ccgacagctc ccccgccacg ctccgggcca tcgcggtgag atccacagac tcgcgccgca
     6361 gctccgctcg cgtgacccgt gcgaggccca gcaggtcctc gatcagctcg gccagatgct
     6421 gggtcgccgc ccgcacccgc cggaggtagt ccttgccgtg ctcgtcgagc agggccgcgc
     6481 agtcttcgag caacgcctgg ctgaagccgt cgatgctgcg cagcggggcc cggaggtcgt
     6541 gcgagaccga gtagctgaag gcttcgagct cctggttgac ggccatgagc tccgcgagct
     6601 tgcgggacag ctcggcattg agtcgctcga tctccgcccg ggagcgctcc cgctcggaga
     6661 tgtcgttgaa ggcggcgacc gcctgcacca ctgtcccctg ccggtcgaag accggcgcgg
     6721 ccagcacctc gatcggcacc atcctgtcgg ggcgacggat ctccatcacc gccggcggcg
     6781 gcttccggcc gcgcagcgct tccatgatcg gctgccggtc ggcagggtag gtgtcgtcgg
     6841 tgccggggac atacgcctgg tagatctccg ggagatcggc tcctgcggct cccacgacga
     6901 tccccttccc caggatcgac tgcgccacct ggttggcgta ggacgatcca cccactgcgt
     6961 ccagcacgaa gacaccgatc ggcatcgcgt ccagaagctg cgcgaggcgt cgctcgctct
     7021 cctgcacggc atcctcggcc cgccgtcgct cggcaagctc ccgaccgacc agaacgctcg
     7081 cggcgatgac cacgaggaac gcgagtgcgg ttccgccgaa caccgagaga cgcgccaagc
     7141 gcgcccggcc gtcgaggatg gccacccgcc ggagtgccag tcgctcgctc cggtcctcca
     7201 tgcgggtggc cagcctgcgc acctggtccg cgatcgacgt cgccccgatc gacgcttcgc
     7261 catcccgcgc cggcgcgcgc gccccgtgcg ggcgggcgag cagggtccgg agcgcgccga
     7321 ttctcagaga gatcgactgc cggagcgagt ccaactgggc ccgctcgt
//
LOCUS       DBNGZW010000726         2342 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_544754, whole genome shotgun sequence.
ACCESSION   DBNGZW010000726 DBNGZW010000000
VERSION     DBNGZW010000726.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2342)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_544754"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..996
                     /locus_tag="AC16JC_17075"
     CDS             <1..996
                     /locus_tag="AC16JC_17075"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003619274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR5166467.1"
                     /translation="HAAEHDPEHTNFMVAGPWFHGMWRSPKGDSIGLIALGGHETARE
                     FRETIEAPFFRYYLHGVGEKPSWQATTFQSGSNRWRTYAAWPPPEGRTRNLYLHADGT
                     LSFDAPRAGAAGAGFRAYVSDPTNPVPYRQRPISPTYPGGDWRTWEVADQRFVDGRPD
                     VLTYVSGPLEHDLTVSGEVAADLFASTSGTDADFIVKLIDVYPEAAQPNAWDPDAGPA
                     PGAYAKSVNGYELPIAMEVHRGRYLASFERPRPLVPNRPLEWRIPLRDRDHVFLKGHR
                     LMVQVQSTWFPLIDRNPQQFVASIYRATAADYVKATQRVYSTPALPSHIVLPVVP"
     gene            1003..1857
                     /locus_tag="AC16JC_17080"
     CDS             1003..1857
                     /locus_tag="AC16JC_17080"
                     /inference="COORDINATES: protein motif:HMM:NF042511.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HIR5166468.1"
                     /translation="MTRWGRRAARVLGGLGLAILLVLAGGTILWKRATARAVVLLGTG
                     PLAPARFSADQLAGLPPPVVRYFEFALTPGQPLVRHARVTQAGTFARRRDAWSPFTAV
                     EEFAVWPPGFVWDASIRMGPLLSVRVRDSYIGGEGAMQGAVAGVVSIVHQRGTPEMAG
                     GALLRYLAEGAWLPTALLPSAGVRWEAVDDSTARATLTDATTTVSMDVHFGPEGEIAR
                     IAAQRYRDLDGKAVLTPWAGSFRAYTRIDGMMVPLESEVGWVLPDGWFPYFRARNVKL
                     EYVSRVTR"
     gene            complement(1854..>2342)
                     /locus_tag="AC16JC_17085"
     CDS             complement(1854..>2342)
                     /locus_tag="AC16JC_17085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166469.1"
                     /translation="GVATFQHLNKSGTAYLNGGPFFGAVLDYAPSSAPGLALLGEVAW
                     THHELRGSVPGAGTGVELFSAGGDVGYIYVNTEKLSSAFFGGGGVLFIYEGTTTAAKL
                     EPFSRLGLDARYRISARICLYAQVTGMIYTLSNFPSGSVLAGYDHHQGDAAFGAGMTV
                     RF"
BASE COUNT          345 a          807 c          815 g          375 t
ORIGIN      
        1 catgcggcgg aacacgatcc cgaacacacg aacttcatgg tggccgggcc gtggttccac
       61 ggcatgtggc ggtcccccaa gggcgacagc atcgggctga tcgcgctcgg cggtcacgag
      121 acggcccgcg agttccgcga gacgatcgaa gcgccgttct tccgctatta tctccatgga
      181 gtgggcgaga agccgagctg gcaggcgacg acgtttcagt ccggctcgaa ccgctggcgc
      241 acctacgccg cctggccgcc ccctgagggc cggacgagga acctctacct gcatgccgac
      301 gggacgctgt ccttcgacgc gccgcgggcg ggagcggcgg gagcgggatt ccgcgcgtac
      361 gtctcggatc ccacgaatcc ggtcccttac cggcagcggc ccatttcgcc gacctacccc
      421 gggggtgact ggcgcacctg ggaagtcgcc gaccagcgct tcgtggacgg tcgccccgac
      481 gtgctgacgt acgtcagcgg gccgctcgag cacgatctca cggtgagcgg tgaggtggcc
      541 gccgacctct tcgcctccac ctccggcacc gatgccgact tcattgtcaa gctgatcgac
      601 gtgtacccgg aggccgccca gccgaacgcc tgggatcccg acgccggtcc ggctcccggc
      661 gcctacgcga aatcggtgaa cggctacgag ttgccgatcg caatggaggt ccaccgcggg
      721 cgctatctcg cgagcttcga gcgtccgcgc cccctcgtcc cgaaccgccc gctggaatgg
      781 cgcattccgt tgcgggatcg cgaccacgtg tttctcaagg ggcaccggct gatggtccag
      841 gtccagtcga cgtggttccc gctgatcgac cggaacccgc agcagttcgt ggccagcatc
      901 taccgcgcga cggccgccga ctacgtgaag gcgacgcagc gcgtctacag caccccggcg
      961 ctgccctcgc acatcgtcct gccggtcgtg ccctgagccg ccatgacccg ctggggcagg
     1021 agggcggcgc gcgtgctcgg cgggcttggc ctggcgatcc tcctcgtgct ggccggtggc
     1081 acgatcctgt ggaagcgggc gacggcgcgc gccgtcgtcc tgctcgggac ggggccactc
     1141 gcgccggcgc gcttttctgc cgaccaactc gccggactgc cgccgcccgt ggtgcgctac
     1201 ttcgagttcg cgctcactcc cgggcagccg ctggtgcgac acgcccgggt gacgcaggcg
     1261 gggacgttcg cgcgcagacg tgatgcctgg tcgccgttca ccgccgtcga ggaatttgcg
     1321 gtgtggcccc ccgggttcgt gtgggatgcg tcgattcgca tggggccgct actttcggtc
     1381 cgcgtccgcg acagctacat cggcggcgag ggagccatgc agggggccgt tgcgggcgtg
     1441 gtctcgatcg tccaccagcg aggcacaccg gagatggctg gcggcgccct gttgcgatac
     1501 ctggcggagg gtgcctggct gccgacggcg ctgctgccga gcgcgggcgt gcggtgggag
     1561 gccgtggacg acagcaccgc gcgggccacg ctgaccgacg ctaccacgac ggtgtcgatg
     1621 gacgtgcact tcggtcccga gggcgagatc gcccgcattg cggcgcagcg ctatcgcgac
     1681 ctcgacggga aagccgtgct gacgccgtgg gcgggctcct ttcgtgcata cacgcgcatt
     1741 gacgggatga tggtgccgct ggagagcgag gtggggtggg tgctccccga cggctggttt
     1801 ccttactttc gagcgcgcaa cgtcaagctg gaatacgtgt cccgagtcac gcgctagaat
     1861 cgcaccgtca tgccggcccc gaacgcggcg tcgccctggt gatgatcgta tcccgcgagg
     1921 acgctgccgc tcgggaagtt gctgagcgtg tagatcatgc ctgtcacctg ggcgtacagg
     1981 cagatccgcg cggagatccg gtagcgggca tcgagcccga ggcggctgaa cggttccagc
     2041 ttcgcggcgg tggtcgttcc ttcgtagatg aagaggaccc cgccacctcc gaagaacgcg
     2101 gaagagagct tctccgtgtt gacgtagatg tagccgacgt cgcctcccgc cgagaacaac
     2161 tccacgcccg ttcccgcccc cggaacggag ccgcgcagct cgtggtgcgt ccaggcgacc
     2221 tcgcccagca aggcgaggcc cggcgcgctc gacggcgcat agtcgagcac ggcgccgaag
     2281 aacggccctc cgttcaggta cgccgtcccc gacttgttca agtgctggaa cgtcgccacg
     2341 cc
//
LOCUS       DBNGZW010000727         4842 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_544839, whole genome shotgun sequence.
ACCESSION   DBNGZW010000727 DBNGZW010000000
VERSION     DBNGZW010000727.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4842)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4842)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4842
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_544839"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..528)
                     /gene="tatC"
                     /locus_tag="AC16JC_17090"
     CDS             complement(<1..528)
                     /gene="tatC"
                     /locus_tag="AC16JC_17090"
                     /inference="COORDINATES: protein motif:HMM:NF013097.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HIR5166470.1"
                     /translation="MPFLDHLEELRWRLLKILLAVVLGTVIGWFVVERVDVIGLLMRP
                     IAPHLPGGRLNFTSPTDPFFITLKFAFIVGVVMTSPYTVFQIWAFLAPALYEREKRLV
                     VPALFSGVLLFLAGAAAGYLLALPHALNFLLGFQNQSLQPIITADRYFTFAAQVIVAV
                     GCTTELPLVIIILAAL"
     gene            complement(567..1355)
                     /gene="ybgF"
                     /locus_tag="AC16JC_17095"
     CDS             complement(567..1355)
                     /gene="ybgF"
                     /locus_tag="AC16JC_17095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HIR5166471.1"
                     /translation="MSRTRAPWLLGLGLLAGCALRGDVRKVELQVEALRSELTRGDSL
                     RRVERDSLARVLASVQATLAAEQNALVQIRGDIRSDLQAVQQQLGSLQELTGQSQQRL
                     SELRSRLAAPAPTPAPAPVDTTGAAPPPAAAPPASGPGAGQMYDLTLQQYRRGSFTTA
                     RLGFREFLRLYPDDEHAPDAFYYIGESFAAENPDSAAAVYQEVVKRYPNSPRAPSALY
                     KLGLLAEQRGDKAAARTYYARVLAGYPRSDEANLARDKLQTLGR"
     gene            complement(1359..1970)
                     /gene="pal"
                     /locus_tag="AC16JC_17100"
     CDS             complement(1359..1970)
                     /gene="pal"
                     /locus_tag="AC16JC_17100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019369903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HIR5166472.1"
                     /translation="MKRVHSLVLMGFGVALVAGCAKKEPEVAPTPQANADSIAAAERA
                     RADSIAADSARRAQEEAERLARQRAADSAAAVAAAAAEVRNTLLAMIHFDFDKANIRS
                     DDAAMLDQKVAILNANPDLRIRINGHCDERGSDEYNLALGNRRSTAAKNYLVSHGVDA
                     SRIETASYGEERPVAMGHDEDSWAQNRRDEFEILSGGDMLRKP"
     gene            complement(1990..3342)
                     /locus_tag="AC16JC_17105"
     CDS             complement(1990..3342)
                     /locus_tag="AC16JC_17105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166473.1"
                     /translation="MRADQLFLVAAFLSAYPSIRLSAQDTSAIDRGVRIGIVYRPGVR
                     PGVVVLPGSQASLDSVRAIITRDLDYSDRFEVITLPGADGASLGPSAPPAAPPRTAPA
                     LNYPLYRALGADFAVEARRVGDTTVVVVHDVTAGGIRRAFRQVLPALGHAGFRLAVHR
                     VADQVLQATLGAPGIAATRILFVRDGKVYTIDQDGADIGLVSSSDHQAMSPAWARNGR
                     QFSYMEFSEGHGQLFVQEVATHRRVSVVTTGKALDFTPAFSPDGKTLAFSRATEEGTD
                     VYTVNMRDNCCLQRLTRGGFSDNLSPTYSPDGQRIAFVSTRPGLPQIYVMGADGTDQQ
                     LFAPFDYGVTGSSNAPEWSPDGQSVAFHRDVGGTLQVFVLDVRTRSVRQLTSLGRNED
                     PTWAPDSRHLAFVSDRSGYRQLWVIDLETGRIRPLLQQSGARLPAWSPRIPEAVPTNP
                     "
     gene            complement(3339..4025)
                     /locus_tag="AC16JC_17110"
     CDS             complement(3339..4025)
                     /locus_tag="AC16JC_17110"
                     /inference="COORDINATES: protein motif:HMM:NF024503.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HIR5166474.1"
                     /translation="MSRQGRAVTNVPLGLAGTVVVHGGVLVLLLASASGARSAPPTYR
                     VRLIAAPDLPADARPAPPAVEREAAPVEPPPAAPKPAPKNTAATAAPPPVADTKKREA
                     APRTTPTARPLPGEKPSTGSDVATVSTEGIEFPYPEYLQNVVSQVLRRWQRPLDNTPL
                     DAEVAFMVHRDGTVTDLQFVKRSGNFAFDLEAQGAIEEAGRFKAFGALPDGWVPDVLF
                     VRFYFSGQRR"
     gene            complement(4022..4453)
                     /locus_tag="AC16JC_17115"
     CDS             complement(4022..4453)
                     /locus_tag="AC16JC_17115"
                     /inference="COORDINATES: protein motif:HMM:NF014525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HIR5166475.1"
                     /translation="MAGLVGGGGLRGHGELPLTADINVTSLVDVAFVLLIIFMITAPI
                     MQGGVDVRLPRAEARPIEPKTGLVVTVDRTGRIFLDQSGLSYEDFRATFPAFVRSRNP
                     TGVYLRADGRVAYATVVQVLAVIRATGVNDVGLVAEPETTR"
     gene            complement(4457..>4842)
                     /locus_tag="AC16JC_17120"
     CDS             complement(4457..>4842)
                     /locus_tag="AC16JC_17120"
                     /inference="COORDINATES: protein motif:HMM:NF013760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HIR5166476.1"
                     /translation="TPTLTMMQLEALRMVLAKEVAAERDAAARFIPWLATFGSVSPLL
                     GLLGTVLGVMDAFIGIAVGGSGNIAAVAPGVAEALVTTVAGLAVAVPAVMAYNAYVNR
                     LGLFAGELEGFAQEIIGTLAREGRL"
BASE COUNT          785 a         1642 c         1703 g          712 t
ORIGIN      
        1 gagggcggcg aggatgatga tgaccagcgg gagctcggtc gtgcacccga cggccacgat
       61 gacctgagcc gcaaacgtga agtagcggtc ggccgtgatg atcggctgca gtgactgatt
      121 ctggaacccc agcagaaaat tgagcgcgtg ggggagcgcc agcaggtagc cggctgccgc
      181 tccggcgagg aacaggagga ctcccgagaa cagcgccggc acgacgagcc gcttttcccg
      241 ctcatacagc gccggggcga ggaacgccca gatctgaaac acggtgtacg gcgaggtcat
      301 gaccacgccg acgatgaacg cgaacttcaa cgtgatgaag aagggatcgg tcgggctggt
      361 gaagttgagc cgccccccgg gcaggtgggg cgcgatgggc cgcatcagca acccgatgac
      421 atcgacgcgc tcgacgacga accatccgat cacggtgccc aggaccacgg ccagcagaat
      481 cttgagcaga cgccagcgta attcctcgag gtgatcgagg aacggcattt cgccgcggga
      541 agaagcgtcg gccacgggag gaggggctag cgccccaggg tttgcagctt gtcgcgcgcg
      601 aggttggctt cgtcggagcg ggggtatccg gccaggacgc gcgcatagta ggtgcgtgcg
      661 gcggccttgt ccccccgttg ctcggcgagg aggcccagct tgtacagggc cgagggcgcc
      721 cgcggcgaat tgggatagcg cttcaccact tcctggtaca ccgcggcggc ggagtcggga
      781 ttctccgcgg cgaacgactc cccgatgtaa tagaacgcgt ccggggcgtg ctcgtcgtcg
      841 ggatagaggc ggaggaactc ccggaagccg agccgtgccg tcgtgaagct cccccgccgg
      901 tactgctgca aggtcaggtc gtacatctga ccggcgcccg gcccactggc gggcggcgcc
      961 gccgcaggtg gcggggcggc gccggtggta tcgaccggcg ccggcgcggg cgtcggggcc
     1021 ggagccgcca gccggctgcg gagctcggac agccgctgct ggctctgccc ggtcagctcc
     1081 tgcagcgacc ccagctgctg ctgtaccgcc tgcaggtcgc tgcggatatc acctcggatc
     1141 tgcacgagcg cgttctgctc ggcggccagc gtcgcctgca ccgaggcgag cacgcgggcg
     1201 agcgagtccc gctccacgcg ccgcaggctg tcgccacggg tcaactcgct ccgcagcgcc
     1261 tcgacctgga gctccacctt ccggacgtca ccccggaggg cgcatccggc caggagtccc
     1321 aggccgagga gccacggcgc gcgcgtacgc gacacgcgtt acggcttccg cagcatgtcc
     1381 ccgccgctga gaatctcgaa ctcgtcgcgg cggttctggg cccacgaatc ctcgtcgtgc
     1441 cccatggcga cggggcgctc ttccccgtag gacgccgttt cgatccggct ggcgtcgacc
     1501 ccgtggctca ccaggtagtt cttggcggcg gtggagcggc gattcccgag tgcgaggttg
     1561 tattcatcgg agccccgttc gtcgcaatgg ccgttgatgc ggatgcgcag gtcggggttc
     1621 gcgttcagaa tggcgacctt ctgatccagc atggcggcgt catcagaccg gatgttcgcc
     1681 ttgtcgaagt cgaagtgaat catggccagc agcgtattgc gcacctcggc tgccgccgcg
     1741 gcgaccgcgg ccgcggaatc cgccgcgcgc tgccgggcga ggcgctccgc ttcctcttgg
     1801 gcgcgccgcg cggaatcggc ggcgatcgaa tcggcgcgcg cacgctcggc cgcggcgatc
     1861 gaatcggcgt tcgcctgcgg ggtgggagcc acctcgggtt ccttcttcgc gcagccggcg
     1921 acgagcgcca cgccgaaccc catcaggacc agcgagtgga cacgtttcat ggtgccatgt
     1981 tctcccgttt caggggttgg tagggacagc ctccggtatc cgcggagacc aagccggtaa
     2041 gcgtgccccg ctctgctgca gcaagggacg gatgcgcccc gtctccagat cgatcaccca
     2101 caactgccgg tagccgctgc ggtccgagac gaaggccaag tgacggctgt ccggtgccca
     2161 ggtagggtcc tcattgcggc cgagcgacgt cagctggcgc accgaccggg tccgtacatc
     2221 gagcacgaag acctgcagcg tgccgccgac atcgcggtgg aaggccaccg actgtccgtc
     2281 gggagaccat tccggagcgt tcgatgagcc ggtcacccca taatcgaagg gggcgaacaa
     2341 ctgctgatcg gtgccgtctg cgcccatgac gtatatctgc ggcaatccgg gccgcgtcga
     2401 gacgaatgcg atccgctgcc cgtcggggct gtaggtcgga gacaagttgt ccgaaaagcc
     2461 gccgcgggtc aagcgctgca ggcagcaatt gtcgcgcatg ttgaccgtat aaacgtcggt
     2521 cccttcttcg gtcgcccggc tgaacgcgag cgtcttcccg tcaggtgaaa aggcgggagt
     2581 gaagtcgagc gctttgccgg tcgtgacgac gctcacgcga cggtgggtcg ccacctcctg
     2641 cacgaacaac tgcccgtgac cctcggagaa ttccatatag ctgaactgcc gtccgttccg
     2701 ggcccatgcc ggcgacatcg cctgatgatc ggaggaagag accaggccga tatccgcccc
     2761 atcctgatcg atggtgtaca ccttgccgtc gcggacgaag aggatgcggg tggcggcgat
     2821 acccggcgca ccgagcgtcg cctgcagcac ctggtcggcc acccggtgca cggccagccg
     2881 gaaccccgca tggccgaggg ccggcaacac ctgacggaac gcacggcgga taccgcccgc
     2941 cgtgacgtcg tggacgacga cgaccgtggt gtcgcccacc cggcgggctt cgaccgcgaa
     3001 atccgctccc agtgcccgat agagcgggta gttcagagcg ggggccgtcc gcggtggtgc
     3061 ggcgggaggt gccgacggcc cgaggctcgc cccatcggcg cccggcaggg tgatcacttc
     3121 gaagcggtcg ctgtagtcca ggtcgcgggt gatgatggcc cgcaccgagt ccaggctcgc
     3181 ctgactcccc ggcaggacga cgacgccggg gcgcacgccg ggccgataca cgatgccgat
     3241 ccggacgccg cggtcgatgg cggaggtgtc ctgggcggat aggcggatcg acggataggc
     3301 ggacaggaat gccgccacca ggaacagctg atccgccctc atcggcgctg ccccgagaag
     3361 tagaatcgca cgaagagcac gtccggtacc cacccgtccg gcagcgcgcc gaatgccttg
     3421 aaccgtccgg cttcctcgat cgcgccctgg gcttcgagat cgaatgcaaa gttccccgac
     3481 cgcttcacga actgcaggtc cgtgaccgtg ccgtcccgat gcaccatgaa agcgacctcg
     3541 gcatcgagcg gcgtgttgtc cagcgggcgc tgccagcggc gcaacacctg ggacacgacg
     3601 ttctggaggt actcgggata cgggaactcg atgccttcgg tgctgacggt cgcgacgtcg
     3661 ctgccggtac tcggcttctc gcccggcagc gggcgcgcgg tcggcgtcgt ccggggggcg
     3721 gcctcgcgct tcttcgtgtc cgccacgggc ggcggcgctg cggtcgcggc ggtgttcttg
     3781 ggtgcgggct tcggtgcggc tggtggcggt tccacgggtg cggcctcccg ctcgaccgcc
     3841 ggcggcgcgg gccgcgcatc cgcgggcaga tccggcgccg cgatgagccg cacgcggtac
     3901 gtgggcggcg ccgagcgcgc gcccgaggcg gacgccagga gcaggacgag cacaccgccg
     3961 tgcaccacga cggtaccggc cagtccgagg gggacgttcg tcacggcgcg cccctggcgg
     4021 ctcatcgtgt cgtctccggc tcggctacga gccccacgtc gttcacgccg gtagcccgaa
     4081 tcaccgcgag cacctgcacc accgtcgcat aggcgacgcg gccgtccgcg cgcaggtaga
     4141 cccccgtggg gttccggctt cggacgaacg ccgggaacgt cgcccggaaa tcctcgtagg
     4201 acaacccgga ctgatcgagg aagatgcgac cggtccggtc caccgtcacc accagaccgg
     4261 tcttcggctc gatggggcgc gcctcggccc gcggcagccg gacatcgacg ccgccctgca
     4321 tgatcggcgc ggtgatcatg aagatgatga ggagcacgaa cgctacgtcg acgagactgg
     4381 tgacgttgat gtccgcggtg agcggcaact cgccgtgccc ccgcagtccg ccgccgccca
     4441 cgagacccgc catcgcctag agcctccctt cgcgcgccaa ggtgccgatg atttcctggg
     4501 cgaagccctc cagctctccc gcaaacagtc ccaggcggtt gacgtaggcg ttgtacgcca
     4561 tgacggccgg cacggcgacc gccaggcccg cgacggtggt cacgagggct tcggcgacgc
     4621 ccggcgcgac cgcggcgatg ttgcccgagc ccccgacggc gatgccgatg aacgcgtcca
     4681 tcacgccgag caccgtgccc aggagcccga gcagcggact gaccgagccg aacgtggcca
     4741 gccacgggat gaagcgggcg gcggcatcgc gctcggcagc gacctccttg gcgagcacca
     4801 tgcgcagcgc ctcgagctgc atcatcgtca acgtcggcgt gg
//
LOCUS       DBNGZW010000728         4230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1499613, whole genome shotgun sequence.
ACCESSION   DBNGZW010000728 DBNGZW010000000
VERSION     DBNGZW010000728.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4230
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1499613"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="AC16JC_17125"
     CDS             complement(<1..362)
                     /locus_tag="AC16JC_17125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166477.1"
                     /translation="MRSTTYAKYLPELADTINLQGLLDQLADFLLQSGFAGGPYNHPF
                     WGEVGGDDADRSMDALRQAILQALMDSGQLTPEMLKVLRGESTGDAQRDAELERQLAE
                     LLDKIVQRLMEEGYLNVS"
     gene            complement(421..1668)
                     /locus_tag="AC16JC_17130"
     CDS             complement(421..1668)
                     /locus_tag="AC16JC_17130"
                     /inference="COORDINATES: protein motif:HMM:NF024816.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPR end-of-group domain-containing protein"
                     /protein_id="HIR5166478.1"
                     /translation="MSPQRDQEYFCEGIAEELINALMRIDELRVASRTSAFAFKGKSE
                     DIREIGRKLQVDHVLEGSVRKAGNRLRITAQLLKVADGYQLWSERYDRELEDVFAIQD
                     EIAQNIARALRVVMTDQAKAIVKPQTADVAAYDYYLRGRGFFHQWRKKSLLFARQMFT
                     RAIEIDAGYALAYAGIADCCSSLYTWFDANETYREEAERTSLRALELDPGLAEAHEAR
                     GVALTVAKRWDEARREFETAIKLSTTLFEAYYFYPRLCFLQGNLEEAGRWFAEAARVR
                     PEDYQALSLLGTIYADLGRAEDARTAFERSYQVVEKHVEMYPDDARAYYMGALAAIAL
                     GRREQAEAWVGRALAIDPEDPLILYNVGCVYARLGKTEQALDCLERTVAHGGTGHRGW
                     MRNDGDLASLRGNPRFEAMIRAE"
     gene            complement(1737..2591)
                     /locus_tag="AC16JC_17135"
     CDS             complement(1737..2591)
                     /locus_tag="AC16JC_17135"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5166479.1"
                     /translation="MSLFEKVQAALADRYTIEREIGRGGMATVYLARDRKHDRRVAVK
                     VLHPDLAASLGPDRFLREIKVAARLSHPHIVSLHDSGQAGEFLYYVMPYIEGESLRQR
                     LNREKQLPLDEALEIARHVASALGYAHSTGVVHRDIKPENVMLHQGEAVVTDFGIAKA
                     VTSAAGEHLTQTGSTVGTPAYMSPEQAGGAEDIDGRSDVYSLGAYCTRCSPAATRSRP
                     RRRRRCWSSTSPSRCRRCGRCVPGSPRASSEPSRRPSPRSRPSASPRRPSSPRHCTPD
                     PLLPASRR"
     gene            2665..3903
                     /locus_tag="AC16JC_17140"
     CDS             2665..3903
                     /locus_tag="AC16JC_17140"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR5166480.1"
                     /translation="MTEAAPERSAARVGLGALAHRNFRLFLFGQGTSLVGTWMQSVAL
                     GWLVLQLTNSPFAIGLNSALRALGVLLFTLYAGIVADRVDKRRLVILTQALQMVEALA
                     LAVLVWTHVVAVWQVMVLALFVGIVNAFDIPTRQAFMVEMVGKEDLMNAIAWNSSMFN
                     AARVVGPAVGGALIGLLGLGMCFFLNGVSYIAVIASLAVMRLPPFQPRPVEGSAWSGF
                     REVVAFLRGDRRVKTLVIQTAILSVFGFPFLVMMPVFARDVLHVQAAGYGALTAAVGV
                     GAMLGALAIALNSGRIVRKGALMTVGGTSFGLLVAVFALSRGFALSLVLLALAGCAMI
                     VNNALTNTMLQTLVPDQLRGRVMGFYSFVFVGLSPLGAFLVGAFAERYGAPVAVGTGG
                     LICALAVATAAWRLPELRRS"
     gene            complement(3916..>4230)
                     /locus_tag="AC16JC_17145"
     CDS             complement(3916..>4230)
                     /locus_tag="AC16JC_17145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166481.1"
                     /translation="PGRHAASFVQHVDSTESWLAEVDGETHAVRRLVRLPRGADFHAW
                     TPGGWVLTSDGATLYQWDSHAAGPWVPVADLAALGLRGTTRLAVSPRGDRLALVAPDP
                     SP"
BASE COUNT          574 a         1419 c         1503 g          734 t
ORIGIN      
        1 tcggagacgt tgaggtagcc ctcttccatg aggcgttgca cgatcttgtc gagcagctcg
       61 gcgagctgcc gctcgagctc ggcgtcgcgc tgcgcgtccc cggtagactc gccgcgcagc
      121 accttgagca tctccggggt gagctgcccc gagtccatga gggcctggag gatcgcctgg
      181 cgcagggcgt ccatcgagcg gtcggcgtcg tcgcccccga cctcgcccca gaaggggtgg
      241 ttgtacgggc cacccgcgaa ccccgactgc agcaggaagt cggcgagctg gtcgagcagg
      301 ccctggagat tgatcgtgtc ggccagctcg gggaggtact tggcgtaagt ggtggagcgc
      361 atgacctagg gaatgtggct tccccggggg gcggaaagca accgggttgt ccggggagcg
      421 ctactcggcc cggatcatcg cctcgaaccg cgggttcccg cgcagtgacg cgaggtcgcc
      481 gtcgttccgc atccagccgc ggtggccggt gccgccgtgg gcgactgttc gctccaggca
      541 atcgagggct tgctcggtct tgccgagccg cgcgtacacg caaccgacgt tgtacaggat
      601 cagcggatcc tcggggtcga tcgccagcgc ccgccccacc caggcctccg cctgctcgcg
      661 ccgccccaac gcgatcgcag ctagcgcgcc catgtaatag gcccgggcgt cgtcgggata
      721 catctccacg tgcttttcga caacctgata cgagcgttcg aacgccgtcc tggcgtcctc
      781 cgcccggccc aggtctgcgt agatcgtgcc cagcagcgac agggcctggt agtcctccgg
      841 acgaacccgc gccgcttctg cgaaccagcg tcccgcctcc tccaggttgc cctggagaaa
      901 gcacaggcgt gggtagaagt aataggcctc gaacagcgtg gtgctgagct tgatcgcggt
      961 ctcgaactcg cgtctggctt cgtcccatcg cttggcgacg gtgagcgcga cgccccgggc
     1021 ctcgtgcgcc tcggccaggc cgggatccag ctcgagcgcg cgcaggctcg tccgctccgc
     1081 ctcctcgcgg taggtctcgt tggcgtcgaa ccaggtgtag agggaagagc agcagtcggc
     1141 gatcccggca taggccagcg catagcccgc gtcgatttcg atggcgcgcg tgaacatctg
     1201 gcgggcgaac agcaggctct tcttgcgcca ttggtggaag aacccgcggc cccgcaggta
     1261 gtagtcgtag gcggcgacat cggccgtctg cggcttgacg atcgctttcg cctggtcggt
     1321 catcacgacc cgcagcgccc gcgcgatgtt ctgggcgatc tcgtcctgaa tggcgaacac
     1381 gtcctcgagc tcgcggtcgt agcgctccga ccacagctga tagccgtcgg ccaccttcag
     1441 gagctgggcc gtgatgcgga gccggttccc ggccttgcgt acactgcctt cgagcacgtg
     1501 gtccacctgg agcttgcgac cgatctcgcg gatgtcctcg ctcttgccct tgaacgcgaa
     1561 cgcgctggtg cgcgaggcga cccgcagctc gtcgatccgc atcagggcgt tgatcagctc
     1621 ctcggcgatc ccctcgcaga agtactcctg gtcgcgctgc ggactcatgt cggcgaacgg
     1681 gagcaccgcc acggacttgg caaccggagc ggacttgccg gcgaccaccg gctggctcac
     1741 cgtcgagacg ccgggaggag tggatccgga gtgcagtgcc ggggcgaact cggtcgccgt
     1801 ggcgaagcgc tcggccggct ccttggcgag ggccttcgtg acggttcgct cgacgccctc
     1861 ggggagcccg gcacgcaccg cccgcagcga cggcaccggc tgggtgaggt gcttgaccag
     1921 catcgcctgc ggcgtcgtgg cctcgaacgg gtggcggccg gcgagcatct cgtacagtac
     1981 gcacccaggc tgtaaacgtc gctgcggccg tcgatgtcct ccgcgccgcc ggcctgttcg
     2041 gggctcatgt acgccggcgt tcccaccgtg gaaccggttt gcgtcaggtg ctccccggcg
     2101 gccgaggtca ccgctttggc gatgccgaaa tcggtgacga ccgcttcgcc ctgatgcagc
     2161 atcacgttct cgggcttgat gtcccggtgg accacgcccg tgctgtgggc gtatcccagg
     2221 gcactcgcca cgtggcgcgc gatctcgagc gcctcgtcca gcggcagctg cttctcccgg
     2281 ttgaggcgct gccgcagtga ttcgccctcg atgtacggca tgacgtaata gaggaactcg
     2341 cccgcctgac ccgagtcgtg cagcgatacg atgtggggat gagagagccg cgcggcaacc
     2401 ttgatctcac gcaggaaccg gtcgggcccg agcgaggccg cgaggtcggg gtgcagcacc
     2461 ttcacggcga cccggcggtc gtgcttccgg tcccgggcga ggtagaccgt ggccatgcca
     2521 ccgcggccga tctcgcgctc gatcgtgtag cggtcggcca gcgcagcttg aaccttttcg
     2581 aacagcgaca ttccgggggt cgtcggtcag ggcaggaaag gatatccatt cgatcggcgg
     2641 gcggccagag ctattttccc cgccatgacg gaagcggcgc cggagcgcag tgcggcacga
     2701 gtcggccttg gcgccctcgc ccaccgcaac ttccggctgt tcctgttcgg tcagggcacc
     2761 tccctcgtcg gcacctggat gcagtcggtg gcgctcgggt ggctggtgct ccagctcacc
     2821 aactcgccgt tcgccatcgg actgaactcg gcgctacgcg ccctgggcgt gctgctgttc
     2881 acgctgtacg ccggcatcgt cgccgaccgg gtggacaaac gccggctcgt catcctcacc
     2941 caggcgctgc agatggtcga ggccctggcc ctcgcggtgc tggtgtggac gcacgtggtc
     3001 gcggtgtggc aggtcatggt gctggcgctg ttcgtgggca tcgtcaacgc gttcgacatc
     3061 cccacgcggc aggcgttcat ggtcgagatg gtcgggaagg aagacctgat gaacgccatc
     3121 gcgtggaact cgtcgatgtt caacgcggcg cgggtggtgg ggcccgcggt cgggggcgcg
     3181 ctgatcggcc tgctcggtct ggggatgtgc ttcttcctga acggcgtgag ctacatcgcg
     3241 gtgatcgcca gcctggccgt gatgcgcctc ccgccgtttc aaccccggcc ggtcgagggc
     3301 tcggcctgga gcgggttccg ggaggtcgtc gcgttcctgc gtggcgaccg gcgcgtgaag
     3361 acgctggtga tccagacggc gatcctcagc gtgttcgggt tcccgttcct cgtcatgatg
     3421 cccgtgttcg cccgggacgt gctgcacgtg caggcggcgg gctacggcgc gctcaccgcg
     3481 gccgtggggg tcggggcgat gctgggcgcg ctggcgatcg ccctgaacag cgggcgcatc
     3541 gtccgcaagg gcgcgctgat gaccgtcggc ggaacgtcgt tcgggctcct ggtggcggta
     3601 ttcgcgctgt cgcggggctt tgcgctttcg ctcgtcctgc tcgccctcgc ggggtgcgcc
     3661 atgatcgtga acaacgcgct gaccaacacc atgctccaga cgctcgttcc cgaccagctg
     3721 cgtggccggg tgatggggtt ttattcgttc gtgttcgtcg ggctgtcgcc gctgggggcg
     3781 tttctggtgg gggcgttcgc ggagcggtac ggagcgcccg tggccgtggg gacgggtgga
     3841 ctgatctgtg cgctggccgt cgccaccgcg gcgtggcggc tgcccgagct gcggcggtcc
     3901 tgagcgcccc tcgcctcagg gagacggatc gggagcgacc agcgccagcc ggtcgccgcg
     3961 ggggctcacg gcgaggcgcg tggtcccgcg caggccgagg gcggcaagat cggcgaccgg
     4021 cacccagggt cctgccgcgt gcgaatccca ttgatacagc gtcgcgccgt cgctcgtgag
     4081 cacccacccg ccgggggtcc aggcatggaa gtccgcgccg cgggggagcc gcaccagacg
     4141 ccgcaccgcg tgggtctcgc cgtcgacctc tgccagccat gactccgtcg agtccacgtg
     4201 ctgcacgaag ctcgcggcgt gccggccggg
//
LOCUS       DBNGZW010000729         4355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_734562, whole genome shotgun sequence.
ACCESSION   DBNGZW010000729 DBNGZW010000000
VERSION     DBNGZW010000729.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4355
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_734562"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1176)
                     /locus_tag="AC16JC_17150"
     CDS             complement(<1..1176)
                     /locus_tag="AC16JC_17150"
                     /EC_number="5.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HIR5166482.1"
                     /translation="MPSQARREIAPAQGKLGVLLVGLGAVATTFIAGVETVRRGLGLP
                     IGSLTQMGTIRLGKRTDRRAPKIREFVPLADLKDLVFAAWDPIPDDAYTAAYKAGVLE
                     PRHLEPIADFLKGIKPMPAVFDQNYVKRLQGTNVKRGKTKRELAEALREDIRAFKQRA
                     GCDRLVLVWCASTEIFLTPGPAHQTLAAFEQAMEQNDPAIAPSMLYAWAALMEGVPFA
                     NGAPNLTVDIPAIMSLAEERRVPIGGKDFKTGQTMMKTVLAPAFKARMLGLAGWYSTN
                     ILGNRDGEVLDDPESFKTKEESKLGVLEYILQPDQYPELYGNVFHKVRINYYPPRGDN
                     KEGWDNIDIFGWLGYPMQIKVDFLCRDSILAAPIVLDLALFFDLAHRAGLGGVQEWLS
                     "
     gene            complement(1179..1868)
                     /locus_tag="AC16JC_17155"
     CDS             complement(1179..1868)
                     /locus_tag="AC16JC_17155"
                     /inference="COORDINATES: protein motif:HMM:NF013250.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HIR5166483.1"
                     /translation="MNVIPQAVKDGFVRLTGPMVRALVRGHVRPNTITTIGALVVIGS
                     AVAYGMSSVRLGGLLLLLSGLFDILDGQVARQGGQSSAFGAFYDSSLDRVGESALFAG
                     IALHFLRGGLPAAQVALGVMLAIAALSASLLVSYTRARAEGLGIECKVGIAARAERIL
                     VLGAPTLFFGAGPGGRLLLWIMAVLALATAVTVVQRIVHVAGVAAGSRTARSSRRRDT
                     QPGHASLRKGN"
     gene            complement(1917..3149)
                     /locus_tag="AC16JC_17160"
     CDS             complement(1917..3149)
                     /locus_tag="AC16JC_17160"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF018743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033922 - peptidoglycan
                     beta-N-acetylmuramidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exo-beta-N-acetylmuramidase NamZ family protein"
                     /protein_id="HIR5166484.1"
                     /translation="MRAHGLWVLGIVACSGPPSTAAQVRPGIEVLLTDSLHLVRGRRL
                     GLLTNQTGVDRSGRRDADALRAAGLQLVALFSPEHGFRGQEDRPGLPDAVDSSTGLPI
                     YSLYGGSRAAARAALDSIDVLLIDLQDIGARYYTYPATATALMRDAAVRGKAVVVLDR
                     PDPIGGTLVAGDVRPAVGDPDSQLVGFLPVPMRHGMTLGELLRLANDVLALRADLTVV
                     PVAGWRRDMAYDVTGLPWVRPSPNMPDLESAFHYPGTCLFEGTNLSVGRGTPMAFQMI
                     GAPWLEPPRLVHWLQGVGSGDRSALVGVEVADTQFTPRAPTDGKYDGVAVRGLRFRVT
                     DRARYDPTRLAVALLAGLVALYPDSFQFRSATFDRLAAGPALRVALSAGDAPAQIWDA
                     WSAPLQQFHGTRVKYLLY"
     gene            3138..>4355
                     /locus_tag="AC16JC_17165"
     CDS             3138..>4355
                     /locus_tag="AC16JC_17165"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 protein"
                     /protein_id="HIR5166485.1"
                     /translation="MRSHRWIVLALIVACRPAGPGPAVPPAPFSPVSLDSVLASLSDS
                     QKVGQLIVPWLSGAYTALDDSSFLEAARWVDSLHVGGIIISVGPPLEIAAKLNALQTR
                     SALPLLVSADLEWGAAMRVRGATGFPQIMATGATGDPNDAYTIGAAAADEGRAVGIHV
                     NFAPDADLNNNPANPIINTRSFGEDPRAVSLLVTAYVRGVREQGMLATLKHFPGHGDT
                     ETDSHLGLPVIRADYARLDTVELVPFRAGIAAGADIVMSAHIAFPSLTGGDDPGTLSA
                     AVLTGLLRDSLHFRGMVVTDALTMGAIVAKYGAGEAAVRAFLAGSDLLLMPANPDSAL
                     AAMLGAVAAGRISRERLNGSVRRLLELKKRLGLFEHRTVPLEQVMQKVGTRRWQRAAE
                     DIAVRSITLVRDST"
BASE COUNT          700 a         1587 c         1444 g          624 t
ORIGIN      
        1 cgacagccac tcctgcacgc cgcccagccc ggcgcggtgc gccaggtcga agaacagcgc
       61 caggtcgagc acgatcggcg ccgccagaat cgagtcacgg cacaggaaat cgaccttgat
      121 ctgcatcggg tagcccagcc acccgaagat gtcgatgttg tcccaccctt ccttattgtc
      181 tccgcgcggc gggtagtagt tgatccgcac cttgtggaac acgttgccgt acagctcggg
      241 atactggtcg ggctgcagga tgtactccag cacgccgagc ttcgactcct ccttggtctt
      301 gaacgactcg ggatcgtcca gcacctcgcc gtcccggttg cccaggatgt tggtcgagta
      361 ccagccggcc agaccgagca tccgcgcctt gaaggccggg gcgaggacgg tcttcatcat
      421 cgtctgcccg gtcttgaagt ccttgccgcc gatcggcacg cgccgctctt cggcgagact
      481 catgatggcc gggatgtcca cggtcaggtt gggcgcgccg ttggcgaagg gcacgccctc
      541 catcagcgcc gcccaggcat acagcatcga cggggcgatg gcgggatcgt tctgctccat
      601 cgcctgctcg aacgccgcca gcgtctgatg ggccggcccc ggcgtgagaa agatctccgt
      661 cgacgcgcac cacacgagca ccagacgatc acagccggcg cgctgcttga acgcgcggat
      721 atcctcgcgc agcgcctcgg cgagctcgcg cttggtcttg ccgcgcttga cgttggtccc
      781 ctggagtcgc ttgacgtagt tctggtcgaa cacggcaggc atcggcttga tgcccttcag
      841 aaaatcggcg atcggctcga gatggcgcgg ctcgagcacg cccgccttgt acgccgcggt
      901 gtaggcgtcg tcgggaatcg ggtcccaggc ggcgaagacc aggtccttca gatcggcgag
      961 cgggacgaac tcccggatct tcggcgcgcg gcggtcggtg cgcttgccga gccggatcgt
     1021 gcccatctgc gtgagcgaac cgatcggcag ccccagcccg cggcgcacgg tctcgacccc
     1081 cgcgatgaac gtcgtggcga cggcccccaa ccccacgagc aggacaccca gcttcccctg
     1141 cgccggagcg atctcacggc gagcctgtga cggcacgatc agtttccttt ccgaagtgac
     1201 gcgtgcccgg gctgcgtgtc ccggcgacgc gacgagcgcg ccgtccggct cccggccgcg
     1261 acgccggcca cgtgcacgat ccgctgcacc accgtaaccg ccgtggcgag cgcgagaacg
     1321 gccatgatcc acagcagcaa acgcccgccc ggcccggcgc cgaagaacag ggtcggcgcg
     1381 cccagcacca gaatccgctc cgcccgtgcc gcgatgccca ctttgcactc gatgccgaga
     1441 ccctccgccc gggcccgggt gtaggacacc agcagcgaag cggacagcgc cgcgatcgcc
     1501 agcatgacgc ccagggccac ctgcgccgcg ggcagaccgc cgcgcaggaa gtggagcgcg
     1561 atgccggcga acaaggccga ttcgcctaca cgatcgagtg agctgtcgta gaaggctccg
     1621 aacgccgagc tctgaccgcc ctgccgcgcc acctgcccgt ccagaatgtc gaacagtccg
     1681 gacaacagca gcagcaggcc cccgagccgc accgagctca tcccgtaagc caccgccgac
     1741 ccgatcacga ccagggcccc gatcgtcgtg atcgtattgg gccgcacgtg tccgcgcacc
     1801 agggcccgca ccatcggccc ggtcaggcgc acgaagccgt ccttcacggc ttgcggaatc
     1861 acattcatag ccgggtgaaa gtaacagccc ccggaacgcc cgcaaagggc aaaacgctag
     1921 tagagcaagt acttgacgcg cgttccgtgg aattgctgca acggcgccga ccaggcgtcc
     1981 cagatctgcg ccggcgcgtc gcccgcgctc agcgcgacac ggagcgccgg tcccgccgcg
     2041 agacggtcga aggtcgcgga ccggaactgg aagctgtccg ggtagagcgc cacgaggccg
     2101 gcgaggagcg ccaccgccag acgcgtcggg tcgtagcgcg cgcgatcggt gacgcggaat
     2161 cgcagcccgc gcacggccac gccgtcgtac ttcccgtcgg tgggtgcgcg gggcgtgaac
     2221 tgggtatccg ccacctccac cccgacgagg gctgaccggt ccccgctccc cactccctgg
     2281 agccagtgga cgaggcgggg tggctcgagc cacggcgccc cgatcatctg gaacgccatc
     2341 ggtgtccccc gccccaccga gagattcgtg ccctcgaaga gacacgtccc cgggtagtgg
     2401 aaggcactct cgagatcggg catgttggga gagggtcgga cccagggcag cccggtgacg
     2461 tcgtaggcca tgtcccggcg ccatcccgca acggggacca cggtgaggtc ggcgcgcaac
     2521 gcgagcacgt cgttggcgag gcgcaacagc tcgccgagcg tcatgccgtg ccgcatcggc
     2581 accgggagga aaccgaccag ttgactgtcg ggatcaccca cggcggggcg gacgtcgccc
     2641 gccaccagcg tcccgccgat cgggtcgggg cgatcgagca ccacgacggc cttgccgcgc
     2701 accgcggcgt cgcgcatcag ggctgtcgcc gtggccggat aggtgtagta ccgggcgccg
     2761 atgtcctgga gatcgatgag cagcacgtcg atcgaatcga gggcggcgcg tgcggccgcg
     2821 cgcgagcctc cgtacaggct gtagattggg agaccggtcg aggaatccac ggcgtccggc
     2881 agaccgggac gatcctcctg gccgcggaag ccgtgctcgg gactgaacaa cgccaccagc
     2941 tgcaggccgg cggcgcgcag tgcgtcggcg tcccggcggc cggagcgatc gaccccggtc
     3001 tggttcgtga gcagcccgag gcgccgtccc cggaccaggt gaagagaatc ggtgagcagc
     3061 acctcgatgc cgggccgcac ctgagccgcc gtggacggtg gaccgctgca ggcgactatc
     3121 cccaacaccc agaggccatg cgctcgcatc gctggatcgt cctcgcgttg atcgtggcgt
     3181 gccgccccgc cggtcccgga ccggccgtgc cgcccgcgcc gttctcaccc gtgtcgctgg
     3241 acagcgtgct ggccagcctg tctgacagtc agaaggtcgg ccagctcatc gttccctggc
     3301 tctccggcgc ctacacggcc ctcgacgatt cgtcgtttct cgaagccgcc cgctgggtcg
     3361 actcgctgca cgtcggcggg atcatcatct ccgtcggtcc ccccctcgag atcgcggcca
     3421 aactgaacgc gctgcagacc cgctcggcgc tgccgctgct cgtttcggcc gacctcgaat
     3481 ggggcgccgc catgcgggtc cggggcgcga ccgggtttcc ccagatcatg gccacgggcg
     3541 ccaccggcga cccgaacgac gcctacacca tcggcgccgc cgcggcggac gaaggccgcg
     3601 ccgtcggcat ccacgtgaat ttcgctcctg acgccgatct caacaacaac ccggcgaacc
     3661 cgatcatcaa cacccgatcg ttcggcgagg acccccgtgc cgtcagcctg ctcgtcaccg
     3721 cttatgtgcg cggcgtgcgc gaacagggga tgctggccac gctgaagcac ttccccggcc
     3781 acggcgacac cgagaccgac tcgcatctgg gcctcccggt gatccgagcg gactacgcac
     3841 ggctcgatac cgtcgagctc gtcccgttcc gcgccgggat cgccgcgggt gccgacatcg
     3901 tcatgagcgc ccacatcgcc ttcccttccc tcaccggcgg cgacgacccg gggacgctct
     3961 ctgcggcggt gctcacgggc ctgttgcgcg actcgctcca cttccgcggc atggtggtca
     4021 ccgacgccct gaccatgggc gcgatcgtcg cgaagtacgg cgcgggggag gccgcggtgc
     4081 gggcgttcct cgccggctcc gatctgctcc tgatgcccgc caatcccgat tcggccctcg
     4141 ccgcaatgct cggcgcggtc gcggcgggcc ggatctcccg ggagcgactg aacggctcgg
     4201 tgcggcggct gctcgagctc aagaagcgcc tcgggctgtt cgagcaccgc acagtgcccc
     4261 tcgaacaggt gatgcagaag gtcggcaccc gccgctggca gcgcgccgcg gaggacatcg
     4321 ccgtccgctc gatcaccctg gtgcgcgact cgacg
//
LOCUS       DBNGZW010000730         5257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_734634, whole genome shotgun sequence.
ACCESSION   DBNGZW010000730 DBNGZW010000000
VERSION     DBNGZW010000730.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_734634"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="AC16JC_17170"
     CDS             complement(<1..542)
                     /locus_tag="AC16JC_17170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166486.1"
                     /translation="MRTAATILLVLLSPALAGRAAAQSNAERMTNDRYTRSHDYDLVD
                     QRIALRAFDWDSTSFEGRVVTTLLARRPGLDSVILDAGAKLHIARIADGAGRALRFST
                     HGDTLVVFPPAPVAFGDTARFTITYHGRVENGSGLTFIQPEGRPHRPQQIWSQGEDHN
                     NHYWFPTYDFPNDKMTWSLEA"
     gene            complement(708..926)
                     /locus_tag="AC16JC_17175"
     CDS             complement(708..926)
                     /locus_tag="AC16JC_17175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166487.1"
                     /translation="MSALTMTLSAQVHTGPCLTLPARLHGRWSAEGSTREYCLHDMTG
                     KPLGTIAAPVARPVVGSTAPTVYLRRGH"
     gene            complement(990..1280)
                     /locus_tag="AC16JC_17180"
     CDS             complement(990..1280)
                     /locus_tag="AC16JC_17180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166488.1"
                     /translation="MTAPNERRLTDRRTALDRRASAPRPGPQPAVERRRGERRMGRER
                     RLSILSAEDQIGEALRLLTYVVETGPLPDEERRSLEAAMVRLRFALDRLEQA"
     gene            1363..1866
                     /locus_tag="AC16JC_17185"
     CDS             1363..1866
                     /locus_tag="AC16JC_17185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166489.1"
                     /translation="MMLLMRLLHIGLGVFWAGTVVFSAIFLFPSIADAGPDGAKVGAA
                     LMRRRFMQVMPAVAGLTILSGLYLYWKVSGGFEPSFMHSGMGMTLGFGAAAAIAAFVV
                     GLVVMRPAMERAGALAQSAARAAPAERQAQLAAAQAMRQRGVRAGQVVAVLVALAVSA
                     MALARYM"
     gene            complement(1857..2642)
                     /locus_tag="AC16JC_17190"
     CDS             complement(1857..2642)
                     /locus_tag="AC16JC_17190"
                     /inference="COORDINATES: protein motif:HMM:NF042696.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6597 domain-containing transcriptional
                     factor"
                     /protein_id="HIR5166490.1"
                     /translation="MDDAAPRFARRLPSPDLAPWIVCYWSLGATDAPRFRSRVLPDGS
                     NDLIVDLSGNTRSFVVGAMPQAEVVTLQGRVDLLGVRFRPGGALPFLHHPLDELTGRE
                     VELDALWGRAAGSLADALATEPPAGRIQRLERALRDVVERRWEESLVTRAVECCRRAH
                     GAISVRQVASALGVGERRLERAFARCVGLSPKRLARVLRFLRTVRHIGRTRLAGAPLA
                     LEAGYADQSHFIREFKALAGVTPTEFVAERRAGIVQDESAAHI"
     gene            2682..3461
                     /locus_tag="AC16JC_17195"
     CDS             2682..3461
                     /locus_tag="AC16JC_17195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012734935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR5166491.1"
                     /translation="MNALDLRLTYIGGPTALLELGGVRLLTDPTFDPAGTEYHTSAYT
                     LRKTQDPALLPDAVGALDAVLLSHDHHFDNLDRAGRELLARAPRILTTPAGAERLGGR
                     AMGLAAWQIVDLPAADGRVVRVTGTPCRHGPADGDRGPATGFALAFADRPGETVYVSG
                     DTVWYEGVAEVARRFSVRVALLFMGAARVAAVGPAHLTFTAKEGVSAAQAFPEASIVP
                     LHYEGWEHFSESRPDIERAFRGAGLEHRLHWLEPGRALRLA"
     gene            3605..>5257
                     /locus_tag="AC16JC_17200"
     CDS             3605..>5257
                     /locus_tag="AC16JC_17200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166492.1"
                     /translation="MPSRSMLQRLALATVVLATWRAPLLVSQATLADLRDLTPREHRS
                     AAFVLTSPQAVQIEATGAEPRLERGKKAWQGAEDEDSTTWPAAAWIVDVRTRAVVWDL
                     RTGATVRSAEGLRRFSGTVRFPAGVYEVHYASYVATSVSYSGSFRNLISLRRDRRNNR
                     VRYGGPYVDDGSYRKLGIVVRGPGRAATGGDMDSASRPLASKVVLSLRADTPGVSERR
                     AFEVARPIALEAYAIGELRKDGTFDYGCIQSADTRRRVWQMDYGHTVEAGGARRNRMV
                     RDTIRLPAGRYIAYFVSDDSHDPGEWNAVPPFDPAFWGLTLRVIDPAARAAFHAIVWD
                     PVPQQTIVSLTRIGNDALRSAGFTLRRPLEVRVYALGEGNDPKGEMDDYAWIVNAATL
                     RRVWAMRYDDTQAAGGADKNRLYDGTLRLDAGSYLVYYRSDGSHSYDDWNAAPPPESR
                     YWGVSVFPASGRLDSSIVAPFRRVASGALAEIVQVKNDRHKRETFTLDRPTALHIYAL
                     GEASGEEMADHGWIEDAANGATVWEMTYDSTSYAGGASKNRLF"
BASE COUNT          769 a         1802 c         1901 g          785 t
ORIGIN      
        1 gcctccagcg accaggtcat cttgtcgttg gggaagtcgt aggtggggaa ccagtagtgg
       61 ttgttgtggt cttctccctg gctccagatc tgctgcggac ggtgcggccg cccctccggc
      121 tgaatgaagg tcagcccgct gccgttctcc accctgccgt ggtaggtgat ggtgaaccgc
      181 gcggtgtcgc cgaaggcgac gggcgcgggc gggaatacca ccagtgtgtc gccgtgagtg
      241 gagaagcgta gcgcgcggcc cgcgccgtcg gcgatgcgcg cgatgtgcag cttcgctcct
      301 gcgtcgagga tcacggagtc gagtcccggg cgccgggcaa ggagggtggt gaccacccgg
      361 ccttcgaaag acgtcgaatc ccagtcgaag gcgcgcagcg cgatccgctg atccaccaga
      421 tcgtagtcgt gggagcgtgt gtagcggtcg ttcgtcatgc gctcggcgtt gctctgcgcc
      481 gcggcacgac cggccagcgc gggggagaga aggaccagca ggatcgtggc cgcggtacgc
      541 atcgtcaggt ctccggaggt gaggcagcgt ccacaaccta ggcggcggtc cgcaaggctg
      601 tccagcgcgg gcgcgaggcc cggaagaccc caataaaata aagaagaccc aaaaacgaag
      661 cgcccacccg gtcctgactg cggacgggtg ggcgcgcgtg ccgccgctca gtggccgcgg
      721 cggaggtaca ccgtcggagc cgtcgagccg accaccggac gcgccaccgg cgccgcgatc
      781 gtgccgagcg gctttcccgt catgtcgtgc aggcagtatt cgcgcgtgct gccctccgcg
      841 gaccagcggc cgtgcagccg ggccggcagg gtgaggcagg ggccggtgtg cacctgggcg
      901 gacagcgtca tggtcaaagc gctcatcggg ggctccggtt atccagtcac ccccttagat
      961 gcctcacccc ggcggatggt tgcgccggat caggcttgct cgaggcggtc cagggcgaag
     1021 cgcagccgga ccatcgcggc ctccaggctg cgccgctcct cgtcggggag gggcccggtc
     1081 tccacgacgt acgtgagcag ccggagcgcc tcgccgatct ggtcttccgc ggagaggatg
     1141 gacagccgcc gctcccggcc catgcgccgc tcgccgcggc gacgctcgac ggccggctgc
     1201 ggccccggcc gcggcgcact cgcacggcgg tcgagggcgg tgcggcgatc ggtgagacgt
     1261 cgctcgttcg gggcggtcat ggacccaaat gtgacaccaa atccggcgct cggccatgtt
     1321 aagattgaag cttctccagc ctcgggagcc acggtggatc tggtgatgct gctcatgcgc
     1381 ctgctgcaca tcggactggg cgtgttctgg gccggcacgg tggtcttcag cgccatcttc
     1441 ctcttcccct cgatcgcgga tgcgggtccg gacggggcca aggtcggggc ggccctcatg
     1501 cggcgccggt tcatgcaggt gatgccggcc gtcgcggggc tgacgattct ctccggcctc
     1561 tatctctatt ggaaagtctc gggcgggttc gagccgtcct tcatgcactc cggaatgggc
     1621 atgacgctcg gattcggggc ggcggcggcg atcgctgcgt tcgtggtcgg cctcgtcgtg
     1681 atgcgccccg ccatggagcg ggccggcgcc ctggcgcaaa gcgccgcccg agcggcgccg
     1741 gcggagcgcc aggcccagct cgcagccgcg caggcgatgc gacagcgcgg cgttcgggcc
     1801 ggccaggtcg tggccgtcct ggtcgcgctg gccgtctccg ccatggcgct ggcgcgctat
     1861 atgtgagcgg cgctctcatc ctgcacgatg ccggcgcgcc gctccgccac gaactcggtg
     1921 ggggtcacgc cggccagcgc cttgaactct cggatgaaat gcgactggtc ggcatagccc
     1981 gcctccagcg cgagcggcgc tcccgccagt cgcgtgcgtc cgatgtggcg caccgtacgg
     2041 agaaaccgca ggacgcgcgc cagtcgcttc ggtgacagtc ccacgcaacg cgcgaacgcg
     2101 cgctccaggc gccgctcgcc taccccgagc gcggacgcga cctgacgcac actgatcgcg
     2161 ccgtgcgccc ggcggcagca ctcgaccgcc cgggtcacca gggactcctc ccagcgccgt
     2221 tccacgacgt cgcgcagcgc gcgctcgaga cgttggattc gcccggcagg cggctccgtc
     2281 gcgagggcgt cggccagcga gcccgccgcc cggccccaca acgcgtccag ctcgacctcg
     2341 cgtcccgtca gctcgtccag gggatggtgc aggaacggca gcgcgcctcc gggacggaaa
     2401 cgcacgccca gcaggtccac acgcccctga agcgtcacga cctccgcctg cggcatggcg
     2461 cccacgacga aggaccgggt attcccggag agatcgacga tcaggtcgtt cgagccgtcg
     2521 gggagtaccc ggctcctgaa acgcggcgcg tccgtcgcgc ccagagacca gtagcagacg
     2581 atccagggag cgaggtcggg gctggggagc cggcgcgcga agcgcggagc ggcgtcgtcc
     2641 atgcgaccaa tctaaaccgc ggctaggttt gccgggatct cgtgaacgcc ctcgatctcc
     2701 gtctcaccta catcggcggc cccacggccc tgctggagct tggcggggtg cggttgctga
     2761 ccgacccgac cttcgatccg gcggggacgg agtaccacac gagcgcgtat acgcttcgca
     2821 agacgcagga cccggcgctc ctccccgacg ctgtcggcgc gctggatgcg gtgctcctga
     2881 gccacgatca tcacttcgac aatctcgacc gcgcggggcg ggagctgctc gcgcgcgctc
     2941 ctcgcatcct cacgactccg gccggcgccg agcgcttggg cggccgggcg atgggactgg
     3001 ctgcgtggca gatcgttgac ctgccggccg ccgacggacg ggtggtccgg gtgaccggga
     3061 cgccatgccg ccatggtccc gcggacgggg accgcggccc ggcgaccggc ttcgcgctgg
     3121 ccttcgccga ccgtcccggc gagaccgtct acgtctcggg cgacacggtg tggtacgagg
     3181 gggtggcgga ggtggcccgg cggttctccg tacgagtcgc gttgctgttc atgggagcgg
     3241 cccgcgtcgc cgccgtgggc ccggcgcatc tcacgttcac ggccaaggag ggcgtctcgg
     3301 ccgcgcaggc gtttcccgaa gcttccatcg ttcctctgca ttacgaaggg tgggagcatt
     3361 tctccgaatc ccgccccgat atcgagcgcg cattcagggg tgcgggcctc gagcaccgcc
     3421 tgcactggct cgagccgggc cgggcgctgc ggctggcctg aatcggggcc ggagtccacc
     3481 cggcctgttc gaatcaaatc atcggcctgc tgcgtcgtat cgtgagtttc gggcgggcgc
     3541 gggagcggca accgtccggc cggcgttcgc cgtccaaacc gagatcaccc acggggaacc
     3601 tcctatgccc tcgcgtagca tgctgcaacg gttggccctg gccacggtcg tgctggcgac
     3661 ctggcgcgcg ccgttgctcg tctcacaggc gacgctggcc gacctccggg atctgacgcc
     3721 gcgggagcat cgctccgcag cgttcgtgct gacgtccccg caggccgtgc agatcgaagc
     3781 gacgggggcc gagccgcgac tcgagcgggg caagaaggcc tggcaggggg ccgaggacga
     3841 ggactcgacc acgtggccgg cggcggcctg gatcgtggat gtccggaccc gcgccgtggt
     3901 gtgggatctg cgcaccggcg ccaccgtgcg ctcggccgag ggactgcgtc gcttctccgg
     3961 aacggtgcgg ttcccggccg gcgtgtacga ggtccactac gcgtcgtacg tcgccacgtc
     4021 ggtatcctat tcagggtcgt tcaggaatct catctcgttg cgccgcgacc gccggaacaa
     4081 ccgggtccga tacggcggcc cgtacgtcga tgacggctcc taccggaagc tcgggatcgt
     4141 ggtccgcggg ccggggcgcg cggcgaccgg tggcgacatg gacagcgcgt cgcgtccact
     4201 ggccagcaag gtcgtgctct cgctccgtgc cgacaccccg ggggtctcgg agcgaagggc
     4261 gttcgaggta gcccggccga tcgccctgga ggcgtatgcc atcggggagc tgcgcaagga
     4321 cggcactttc gactacgggt gtatccagag cgccgatacg cgccgccgcg tgtggcaaat
     4381 ggactacggc cacaccgtgg aggccggcgg cgcccggagg aaccgcatgg tgcgcgacac
     4441 catccgcctg ccggccgggc gctacatcgc ctacttcgtg agcgacgact cgcacgaccc
     4501 cggagagtgg aacgccgtgc cgccgttcga ccccgccttc tggggcctga cgttgcgcgt
     4561 catcgacccc gcggcgcggg cggcgttcca cgcgatcgtg tgggaccccg tgccccaaca
     4621 aacgatcgtg tcgctcaccc gtatcggcaa cgacgcgctg cgctccgccg gcttcacgtt
     4681 gcgccggccg ctcgaggtac gggtgtatgc gctgggcgag ggcaacgatc ccaagggaga
     4741 gatggacgac tatgcctgga tcgtgaacgc cgccacgctg cggcgggtct gggcgatgcg
     4801 gtacgacgac acccaggcgg cgggcggcgc ggacaagaac cggttgtacg atgggacact
     4861 ccgtctcgac gccggcagct acctcgtcta ttacaggagc gacggctcgc actcgtacga
     4921 cgactggaac gccgcgccgc cgcccgaaag ccggtactgg ggggtgagtg tctttccggc
     4981 gagcggccgg ctcgattcga gcatcgtcgc cccgttccgg cgagtggcga gtggggcgct
     5041 cgccgagatc gtgcaggtga agaacgaccg gcacaagcgg gagacgttca ccctggaccg
     5101 gccgaccgcg ctgcacatct acgccctggg ggaggcgagc ggagaggaga tggccgatca
     5161 cggctggatc gaggacgccg cgaacggcgc cacggtctgg gagatgacgt acgactccac
     5221 gagctacgcc gggggcgcca gcaaaaaccg gctgttc
//
LOCUS       DBNGZW010000731         2217 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_734835, whole genome shotgun sequence.
ACCESSION   DBNGZW010000731 DBNGZW010000000
VERSION     DBNGZW010000731.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2217)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2217)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2217
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_734835"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="AC16JC_17205"
     CDS             <1..669
                     /locus_tag="AC16JC_17205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166493.1"
                     /translation="RTRDFLRAHQTEVAGEATALARLEELVQRAETLTAQQRAGVVAT
                     RSATMQRTQLREEIQKLLRFLAGVGVVAAKENADLAAQFLLPRTGASNQAFLTAARVM
                     LQKATAQRDVLAVLGLPASLLDDLAAALGEFEKTLEATRAGRRDHVGASADLAAVATE
                     IVEQVRLLDGLVRYRFGDNAELMGAWSSARNVLGPFRSRSEPAAGDGQTPSEAGPDAV
                     KPAA"
     gene            complement(1394..>2217)
                     /locus_tag="AC16JC_17210"
     CDS             complement(1394..>2217)
                     /locus_tag="AC16JC_17210"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0140905 - haloperoxidase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vanadium-dependent haloperoxidase"
                     /protein_id="HIR5166494.1"
                     /translation="AAVFATSMDDGGHEGYKTNFPPYTPPVGPGLWVPTAPGQTALQP
                     YWGTTVQTFVLSSGAECDQGGPPAYSEDPASDFYAEANEVYESVNHLTPNQLTIARFW
                     ADGPGTISGPGHSLAIVNQILVQEGANLAVAAETYARVGIADADEVTAVWWAKYHYNL
                     LRPVTYINHVIDASWTTVLPTPPFPEYTSAHSGQSAAAAASLEYLFGNHVAFVDHTHD
                     ADGFAPRPFERIYAAAEEAGISRIYAGIHYRSANLEGQAQGRCVAAKVDGLNWQR"
BASE COUNT          351 a          746 c          762 g          358 t
ORIGIN      
        1 cggacccggg atttcctccg ggcccatcag acggaagtgg cgggtgaggc gacggcgctc
       61 gctcgcctgg aggagctggt gcagcgcgcc gagacgctca ccgcccagca acgggccggc
      121 gtggtagcga cacgatcggc caccatgcag cggacgcagc tccgggagga gatccagaag
      181 ctcctgagat tcctggccgg ggtgggcgtc gtcgcggcga aggagaacgc cgacctcgcg
      241 gcgcagttcc tgttgccgcg caccggcgcc agtaaccagg ccttcctcac ggcggcgcgc
      301 gtgatgctgc agaaggcgac cgcgcagcgg gacgtgctcg cggtcctggg gttgccggcg
      361 agtctgctcg atgacctcgc cgccgccctg ggggagttcg agaaaaccct ggaggcgacg
      421 cgggccggcc ggcgcgatca tgtgggcgcc agcgccgatc tggcggccgt cgccaccgag
      481 atcgtcgagc aggtgcgatt gctcgatggg ttggtccgct atcggttcgg ggacaacgcc
      541 gagctgatgg gggcgtggag cagcgcccgc aacgtgctgg ggccgttcag gtcccggagc
      601 gagccggccg cgggtgacgg gcagacgccg tcggaggcgg gaccggatgc ggtcaagccg
      661 gcggcgtgag tgtgacggaa cggcgcggta gcctgatcac ctgccgcgcc gcttgcatca
      721 gcctctcctt cgctccttgt acgtcgcgct ctatccccta tctcggtacc tcaccccctg
      781 tccccctctc cccctcgctg cgctcggggc aggctctgtc gggagagggg gaacgcaatg
      841 gatggagttg ccccgatttg gtggacaccg accaagcctc catacttgga ggcgggaggt
      901 gtccgatgcc agggaagcgc cgccgggctt tgggacgagc gtgagaccgc gccttcgacg
      961 gaccacccgc tcgcaccgat gacaaccgcc caaacgagcc agactaccct gttcactcgt
     1021 cgcctcccgg gccctacgcc gatggatgat ttgcccggtg agtttacacc cgcctgtgct
     1081 acgacgcacc tcattctgac gaagcccgca cacggcttcg gtcagaaaat cgctcggttg
     1141 gcgggcgccc gccatcaccg cttcgtccac gtatgcgccc aggaagccgg ccgcgtcgcg
     1201 gcggttgggt gcatcgagca tcccgtgtgc gacgtagaag catcgcgtct ccgggtcgca
     1261 caaaaccagg ggcgacaggc gaagcgggca cgaaaccggt tctcggccaa ccctgagtga
     1321 cgacggagca ggtgagagtg cgccgcgcca ggctcccgcg gcgcacgtct ctgcttactc
     1381 tgagaactgc gattcagcgc tgccagttca gaccatccac cttcgccgcg acacagcgac
     1441 cctgtgcctg cccctccagg ttcgccgatc gataatgaat cccggcgtag atgcgcgaga
     1501 tgcctgcctc ttcggccgcg gcgtagatgc gctcgaacgg tcgcggcgcg aaaccgtctg
     1561 cgtcgtgcgt gtgatccacg aaggcgacgt gattgccgaa cagatactcg agcgaagccg
     1621 ccgccgccgc cgactgcccc gagtgcgccg acgtgtattc ggggaacgga ggggtcggca
     1681 ggaccgtggt ccagctcgcg tcgatgacgt ggttgatgta ggtgaccgga cgcagcaggt
     1741 tgtagtggta cttggcccac cacacggccg tgacttcatc ggcgtccgcg atccccacgc
     1801 gcgcgtacgt ttcggcggcc accgccaggt tcgccccctc ctgcaccagg atctgattca
     1861 cgatcgcgag cgaatggccc ggcccggaga tcgtaccggg gccgtccgcc cagaagcggg
     1921 cgatcgtgag ctggttcggg gtcaggtggt tgaccgactc gtacacctcg ttcgcctccg
     1981 cgtagaaatc cgaggccggg tcttccgagt aggccggcgg gccgccctga tcgcactctg
     2041 cgccgctcga caacacgaag gtctgcaccg tcgttcccca gtacggctgg agcgccgttt
     2101 ggccgggagc cgtgggtacc cacaggccgg gtcccaccgg cggagtgtag ggcgggaagt
     2161 tcgtcttgta tccttcgtga ccaccgtcgt ccatcgatgt cgcgaacacg gccgcgc
//
LOCUS       DBNGZW010000732         2495 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1074235, whole genome shotgun sequence.
ACCESSION   DBNGZW010000732 DBNGZW010000000
VERSION     DBNGZW010000732.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2495)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2495)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2495
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1074235"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            164..>2495
                     /locus_tag="AC16JC_17215"
     CDS             164..>2495
                     /locus_tag="AC16JC_17215"
                     /inference="COORDINATES: protein motif:HMM:NF013592.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HIR5166495.1"
                     /translation="MRPATPLAALVLAFAAGAAAQQPRAAVGAYTALRVWPQEPRRFD
                     LLHQTIRVRFDAPHRELFGEVTTRVVLSAASDTVRLDAENLTIDRASGAGGRSLRFTA
                     DTSHVTVRLARRAAAGDTVEFTLAYHGFPERGLYFVPRRNVIWSQGEATETRAWIPTY
                     DAPNDKTTWEFYVTVDSGLTVLSNGRLAGVAPAAGGKQRVWHWVQDRPASTYLYSVVA
                     GPFVVLKDQWRGRPVEYWTYADTTDAAWRTFGETPAMIETYSQVLGVPFAWDKYDQSI
                     IPDFTYGGMENVSATTQTDLALHGAGGEPDNSGRGLNAHELAHQWFGDLTTTADWADI
                     WLNEGITTYMESVQNEKSRGWDAAALEWGGQQRAAMDADRNEERPLVWGRYEGTDPIV
                     LFFSGHVYPKGAQLAHQLRRLLGDSLFWAGMHRFLVDNAYRAVTTPDYAVAMEKTCNC
                     DLDWFFDQWAYGIGYPRVTFTRHWDAPTKTLHVTVAQTQTVDSLHPLFRFPVTLRVIT
                     RDSVVRQQIMVSKPSEIFAVTLPSEPLSFRFDEGGWLLGTVTGDLSGAELAQMAAHDL
                     DVRGRDWALRQLATVHDSTAQAARRFVVLSEHLAELRQLALEAMGGDSSDATRAVLRA
                     ALRDPDGSVRGAALNALAALDQPGAGAAATRMYTSDPSDAVRQAALGVVARAQAPEAL
                     TLLVAAAGPDQPIGTRFTAISYLRGIHDPKALDALERLTATTEDRNIRTSGLGALAAS
                     DSARGTAVALRLIADPDPLFASTAVRIGARVGGAAAK"
BASE COUNT          366 a          912 c          860 g          357 t
ORIGIN      
        1 cctccgaggg catcctcgtg acctacgcgc tgatctacca ggtgctgggt caggccctgc
       61 agcccaagga tcccgccctc gccacccgcg ctgccgcgat cgcggactcg atcaccaaga
      121 acagcgccaa tttccgcaac acgcttacgc cggaggcgtc cccatgagac ccgccacccc
      181 gctcgccgcg ctcgtgcttg ccttcgcggc gggcgcggcc gcccagcagc cccgcgccgc
      241 cgtcggagcg tacaccgctc tccgggtctg gccgcaggag ccgcgccgct tcgatctgct
      301 gcaccagacg atccgcgtcc gcttcgacgc gccgcatcgc gagctgttcg gtgaagtcac
      361 cacgcgcgta gtgctcagcg ccgcatccga caccgtgcgg ctcgacgccg agaacctcac
      421 gatcgatcgc gccagcggcg cgggcgggcg gtcgctgcgg ttcacggccg acacgagcca
      481 cgtcaccgtc cggctggcgc ggcgcgcggc cgcgggcgat accgtcgaat tcacgctcgc
      541 ctatcacggc ttccccgagc gcgggctcta cttcgtgccg cggcgcaacg tgatctggag
      601 ccagggcgag gcgaccgaga cgcgggcctg gatccccacc tacgacgccc ccaacgacaa
      661 gaccacctgg gagttctacg tcaccgtcga ttccgggttg acggtgctct ccaacgggcg
      721 gctggccggc gtcgcgcccg ccgccggcgg gaagcagcgc gtgtggcact gggtgcagga
      781 tcgcccggcg tccacgtacc tctattccgt cgtggccggg ccgttcgtcg tgctcaagga
      841 tcagtggcgc ggccgtcccg tcgagtactg gacctacgcc gacacgaccg acgccgcctg
      901 gcgcaccttc ggcgagacgc cggccatgat cgagacgtac tcgcaggtgc tcggggtccc
      961 gttcgcctgg gacaagtacg accagtcgat cattcccgac ttcacctacg gcggcatgga
     1021 gaacgtctcg gccacgaccc agaccgatct cgccctgcac ggcgcgggcg gcgagccgga
     1081 caacagcggc cggggactga atgcgcacga gctggcccac cagtggttcg gcgacctcac
     1141 caccaccgcc gactgggccg acatctggct caatgagggc atcaccacct acatggagtc
     1201 ggtgcaaaac gagaagtcgc gcggctggga cgccgcggcg ctggagtggg gcggccagca
     1261 gcgcgccgcg atggacgccg atcgcaacga ggaacggccc ctcgtgtggg ggcgctacga
     1321 aggcaccgac ccgatcgtgc tgttcttcag cggccacgtc tatcccaagg gcgcgcagct
     1381 ggcgcatcag ctgcgccgcc tgctgggcga ttcgctcttc tgggcgggga tgcatcgctt
     1441 tctggtggac aacgcctacc gcgccgtgac cacgcccgac tatgcggtgg cgatggaaaa
     1501 gacgtgcaac tgcgacctcg actggttctt cgaccagtgg gcctacggca tcggctatcc
     1561 gcgggtgacg ttcacgcgcc actgggacgc gcccacgaag acgctgcacg tcacggtcgc
     1621 ccagacgcaa accgtggact cgctccatcc cctgttccgg ttccccgtca cgctgcgcgt
     1681 gatcacgcgc gactcggtcg tgcgccagca gatcatggtc tcgaagccga gcgagatatt
     1741 cgcggtgacg ctcccctcgg agcccctgtc gttccggttc gacgagggcg gctggctgtt
     1801 gggaaccgtc acgggcgatc tctccggcgc ggagctcgcg cagatggccg cgcacgacct
     1861 cgacgtgcgc gggcgggatt gggcgctgcg ccagctcgcg accgtccatg actccacggc
     1921 ccaggcggcc cggcgcttcg tcgtgctgag cgagcacctg gccgagctgc gccagctggc
     1981 gctcgaggcg atgggcggcg attcgagtga cgcgacccgg gccgtgctgc gcgccgcgct
     2041 gcgcgatccc gacggcagcg tgcgcggggc cgcgctgaac gcgctggcgg cgctcgacca
     2101 gcccggggcg ggggcggccg ccacccgcat gtatacgagc gatccgagcg acgccgtgcg
     2161 gcaggccgcc ctgggggtgg tcgcacgggc gcaggcgccg gaggcgctca ccctgctcgt
     2221 ggccgcggcg ggaccggatc agccaatcgg cacacggttc acggccatct cgtatctgcg
     2281 cgggattcac gaccccaagg ccctggacgc cctcgagcgg ctgacggcga ctaccgagga
     2341 ccgcaacatc cgcacgtcgg gactcggcgc gctcgcggcg agcgacagcg cgcgtgggac
     2401 cgcggtggcg ctgcggttga tcgccgaccc cgatcccctg ttcgcctcca cggcggtgcg
     2461 tattggggcg cgggtcggcg gcgcggcggc taagg
//
LOCUS       DBNGZW010000733         4364 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_735009, whole genome shotgun sequence.
ACCESSION   DBNGZW010000733 DBNGZW010000000
VERSION     DBNGZW010000733.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4364
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_735009"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /locus_tag="AC16JC_17220"
     CDS             <1..804
                     /locus_tag="AC16JC_17220"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR5166496.1"
                     /translation="TGGYVAGPVVWRAQRAGLPTALQEQNALPGIATRWLAPRARQIH
                     LGFPEAAARLRPGPRTALFTLGNPIRPPAPLGADDRTTALAAFGLAPERPTVLVFGGS
                     QGARALNYAVAGALEHEGLSDVNLLWGTGPAHASTFARYAVPGRVVVQGFFDPMARAY
                     RAADVVLCRAGAMTVAEVCAWGKASVLVPLPSAAADHQRYNARALADAGAAVLLPETD
                     LSGRTLSATVHGLLQHPERRAALTTQARLRGRPNAATEIMSNILTLADS"
     gene            901..2268
                     /gene="murC"
                     /locus_tag="AC16JC_17225"
     CDS             901..2268
                     /gene="murC"
                     /locus_tag="AC16JC_17225"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR5166497.1"
                     /translation="MGIAGAGMSALALLAKLRGASITGCDNDTSGASDLQALGVPVWQ
                     GHDPGHVEGARAVVVTAAVPADHPELARARALGIAVLRRADALGAVVAGSTVVAVAGT
                     HGKTTTTTMCTQALAAAGRNPTGLVGGRVAAWGGNARLGGTSLYVVEADEYDRSFLSL
                     TPTVAVINNVEADHLECYDGSTAELERAFAQFASPASRTIVGVDDPGAARVAAALTGP
                     VWRIGMGANAGVDVHIRDVELGPDGSRARITLPGGRTVRLRLRVPGLHNVRNATAALA
                     VVAALEADVAPALDALATFTGAARRFERVGEAAGVTIVDDYAHHPSEVRATLAAARQA
                     FPGRRLLAVFQPHLYSRTALHGAALGRELAAADAVFLAPVYAAREQPLPGVTAELVAA
                     GARAAGAAPESVRERTDLAARVARVARSGDVVLTMGAGDVTRTGPELLALLAHGAVAS
                     GGTKP"
     gene            2265..2966
                     /locus_tag="AC16JC_17230"
     CDS             2265..2966
                     /locus_tag="AC16JC_17230"
                     /inference="COORDINATES: protein motif:HMM:NF020067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR5166498.1"
                     /translation="MTPRVRRLLIGAGVVVGAWVVLPHLLRHVGFFRVRQVELVGVRY
                     SAPSAVIDALRLEPNASVFDDLDALAEHLRVLRGIADARVVRHLPGALKVIVREVEPV
                     ALVPGANGPLVTVDAAGRPLPYDPSRVAVDLPVAASADSGLVGLLALVQSMDPTLFEE
                     ITAARASRGDVVLQLGTRRLRMARDAGPDIIRALVLVANDLQSRSRAYGELDACYAGQ
                     IVVRRVAGGDGGGGS"
     gene            2963..4219
                     /gene="ftsA"
                     /locus_tag="AC16JC_17235"
     CDS             2963..4219
                     /gene="ftsA"
                     /locus_tag="AC16JC_17235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HIR5166499.1"
                     /translation="MSRDRRLVAGLDLGSTKTCAVIAEVVGDLPRSPLAKVLGVGLAK
                     NSGVRRGVVRDIEETVRSVTAALRDAERMSGVKAAAVTCGIAGEHVSARNSSGVVAVN
                     GDEITPADVARVNEVARAVSLGPDQELLHDIPQEYMVDQQRGISDPVGMTGMRLEVEA
                     YLVTVQSTAAQNLRKAVQRAGYRVADLVLEPLAASYAVLTDDEKELGVALVEVGGGST
                     GVAIFHEGKIRHVASLKYAGAHVTNDLVQGLGVTQADAERLKERHGIAYAPLVDASEM
                     INLPSTPGQGARQASRELLSHIIHQRLDEIFMLVGREFERAGYGGGRLPAGVVLTGGT
                     SHLPGLVELAREVFAVPVRAGAPELGISGLVDSVQAPRYAVPVGLVLYAARRLMQGAA
                     GHAASAGDGGVEKVLGPLKRWLQDFF"
     gene            4239..>4364
                     /locus_tag="AC16JC_17240"
     CDS             4239..>4364
                     /locus_tag="AC16JC_17240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR5166500.1"
                     /translation="MIFELEETSGQNARMKVVGVGGGGGNALNRMIDEGVQGVEFI"
BASE COUNT          561 a         1445 c         1708 g          650 t
ORIGIN      
        1 acgggcggct atgtggcggg accggtcgtg tggcgcgcgc agcgcgccgg tctgccgacg
       61 gcgctgcagg agcagaacgc cctgccgggg atcgccacgc gctggctggc gccccgcgcg
      121 cgacaaatcc atctcggctt tcccgaagcg gcggcgcgct tgcgtcccgg cccgcgtacc
      181 gcgctgttca ccctgggcaa tccgatccgc ccgcccgcgc cgctcggcgc cgatgaccgc
      241 acgaccgcgc tcgctgcgtt cggtctcgcg cccgagcgcc cgacggtgct cgtgttcggc
      301 ggcagccagg gggcccgcgc cctgaactat gcggtcgccg gcgcgctgga gcacgaggga
      361 ttgagcgacg tcaacctgct gtggggcacc gggccggcac acgccagtac gttcgcgcgt
      421 tacgcggtgc cgggccgcgt cgtggtgcag ggtttcttcg atccgatggc gcgcgcgtat
      481 cgcgctgccg acgtggtgtt gtgccgcgcc ggcgcgatga ccgtggccga ggtgtgcgcg
      541 tgggggaagg cgagcgtact cgtgcccttg ccgtcggccg ccgccgatca tcagcgctac
      601 aacgcgcgcg cgctggcgga tgcgggtgcc gccgtgttgc tgcccgagac cgacctctcc
      661 ggtcgcacgc tgtcggcgac ggtccacgga ctgctccagc acccggagcg gcgagcggcg
      721 ctgaccactc aggcgcgact gcgcgggcgg ccgaacgccg ccaccgagat catgtcaaat
      781 attttgacac tcgccgattc ataaccagtc ggtcttttct aagttgcgta aaatccctat
      841 attagcctcc cacagatgac cttgtttaca cccgacagcg atacccgacc ggtccacttc
      901 atgggcatcg ctggagcggg gatgagtgcc ctggcgttgc tggcaaagtt gcgcggggcg
      961 tccataactg gttgtgacaa cgacacttct ggtgctagcg acttgcaggc gctcggtgtg
     1021 cccgtctggc aggggcacga tccggggcac gtggaggggg cgcgggctgt ggtggtgacg
     1081 gcggcggtcc ccgccgacca ccccgagctc gcccgtgccc gcgcgctcgg catagcggtg
     1141 ctccgccgcg ccgacgcgtt gggggccgtg gtggccggca gcacggtggt cgccgtcgcg
     1201 gggacgcacg gcaagaccac gacgacgacg atgtgcaccc aggcgctggc ggcggcgggg
     1261 cggaatccga cgggcctcgt gggcggtcgc gtggcggcct ggggcggcaa cgcccgcctc
     1321 gggggaacgt cgctctacgt cgtcgaagcg gacgagtacg acaggtcgtt tctctcgctg
     1381 acgccgacgg tggcggtgat caacaacgtc gaggccgatc atctggaatg ttacgacggc
     1441 agcacggccg agctcgagcg ggcgttcgcg caattcgcct cgcccgcgag ccggacgatc
     1501 gtcggcgtgg atgaccccgg ggcggcgcgc gtcgcggcgg cgctcacggg accggtctgg
     1561 cgcatcggga tgggagcgaa cgccggcgtc gacgtccaca tccgcgacgt cgagctgggg
     1621 cccgacgggt cgcgggcgcg cattactctc ccgggcggcc gcaccgtccg gctgcgtctg
     1681 cgcgtgccgg gactgcataa cgtccgcaac gcgacggccg cgctcgcggt cgtcgccgcg
     1741 ctcgaggccg acgtggcgcc cgccctggac gccctggcga cgttcaccgg cgcggcccgg
     1801 cgcttcgagc gcgtgggcga ggcggcgggc gtgaccatcg tggacgacta cgcgcaccat
     1861 ccgagcgaag tccgggcgac gctcgccgcc gcgcgacagg catttccggg gcgccgcttg
     1921 ctggcggtat ttcagccgca cctgtattcg cgcaccgcgc tgcacggcgc cgcgctgggc
     1981 cgggagctgg ccgccgcgga cgcggtattc ctggcgccgg tgtacgcggc gcgcgagcag
     2041 ccgctcccgg gcgtgacggc cgagctggtc gcggcgggcg cgcgcgcggc cggcgcagca
     2101 ccggagtcgg tgcgtgagcg gaccgatctg gcggcgcgcg tggcccgggt ggcgcgatcc
     2161 ggcgatgtcg tgctcaccat gggcgcgggc gacgtgacgc gcaccggtcc ggagctcctg
     2221 gcgctgctgg cgcacggcgc ggtggcgagc ggagggacca aaccgtgacc ccgcgcgtgc
     2281 ggcgcctcct gatcggggcc ggcgtggtcg tgggcgcctg ggtggtcctg ccgcacctgt
     2341 tgcgccacgt gggattcttc cgggtgcgtc aggtcgagct ggtgggggtc cgctactcgg
     2401 ccccgtccgc ggtgatcgac gcgctccgcc tcgagccgaa cgccagcgtg ttcgacgatc
     2461 tcgatgcgct cgcggagcac ctgcgggtgc tccgcgggat cgccgatgcc cgggtcgtgc
     2521 gtcatctgcc gggggcgctg aaggtgatcg tgcgcgaggt cgagccggtg gcgctcgtgc
     2581 cgggcgcgaa cggcccgctg gtgacggtgg acgcggcggg acgcccgctg ccgtacgatc
     2641 cctcgcgagt cgccgtggat ctgcccgtgg ccgcgagcgc cgactcgggg ctggtggggc
     2701 tgctggccct ggtgcagtcg atggatccca ccctgttcga ggagatcacg gcggcccgtg
     2761 cctcgcgcgg cgacgtcgtg ctgcagctgg ggacgcgccg tctgcggatg gcgcgcgacg
     2821 ccgggccgga catcatccgc gcgctggtgc tggtcgccaa cgatctccaa tcgcggtcgc
     2881 gcgcctacgg ggagctcgat gcgtgctatg ccggccagat cgtggtgcgg cgcgtggcgg
     2941 gcggggacgg aggggggggc tcgtgagccg cgatcgtcgc ctggtggccg gcctggatct
     3001 cggcagcacc aagacgtgcg ccgtgatcgc ggaagtcgtg ggcgatctgc cgcgctcccc
     3061 gctcgccaag gtgctgggcg ttggtctcgc caagaacagc ggcgtgcgcc gcggcgtggt
     3121 gcgcgacatc gaggagacgg tgcgctcggt gacggcggcg ctgcgggatg cggagcgcat
     3181 gtcgggcgtg aaggcggcgg ccgtgacgtg cggcatcgcg ggcgagcacg tgtcggcgcg
     3241 gaactcctcg ggtgtggtgg ccgtcaacgg cgacgagatc acccccgccg acgtggcgcg
     3301 cgtgaacgaa gtcgcccgcg cggtgtcgct cgggcccgac caggagctgc tgcacgacat
     3361 cccccaggaa tacatggtgg atcagcagcg cggcatctcc gacccggtgg ggatgacagg
     3421 gatgcggctg gaggtcgaag cctacctggt cacggtgcag tccaccgccg cccagaacct
     3481 gcgcaaggcc gtgcagcggg cgggctatcg cgtagccgat ctggtgctcg agccgctcgc
     3541 ggcgtcctac gccgtgctga ccgacgacga gaaggagctg ggcgtcgcgc tggtggaggt
     3601 cggcggcggc tcgaccgggg tcgcgatctt ccacgaaggg aagatccggc acgtggcgtc
     3661 gctcaagtac gccggggcgc acgtgaccaa cgatctcgtg caggggctcg gcgtgacgca
     3721 ggccgacgcc gagcggctca aggagcggca cggcatcgcg tacgcgccgc tggtggatgc
     3781 ctcggaaatg atcaacctgc cgtccacgcc ggggcagggg gcgcggcagg cgtcgcgcga
     3841 gctgttgtcc cacatcattc atcagcggct cgacgagatt ttcatgctgg tgggtcggga
     3901 atttgagcgc gccggttacg gcggcggccg actccccgcg ggcgtggtgc tcaccggcgg
     3961 gacgtcgcat ctgcccggcc tggtggagct ggcccgcgag gtcttcgcgg tcccggtgcg
     4021 ggcgggggcg cccgagctgg gcatctccgg tctggtggac agcgtgcagg cgccgcgcta
     4081 cgcggtcccg gtggggctgg tgttgtacgc ggcgcggcga ttgatgcagg gcgcggcggg
     4141 acacgccgcg agtgcggggg acggcggagt cgaaaaggta ctggggccgc tgaaacggtg
     4201 gctgcaggat ttcttctgat cgagggcccg agggagccat gatcttcgaa ctggaagaaa
     4261 catccggaca gaacgcgcgg atgaaagtcg tcggcgtcgg cggcggcggc ggcaacgcgt
     4321 tgaaccgcat gatcgacgag ggcgtgcagg gcgtcgagtt catc
//
LOCUS       DBNGZW010000734         1939 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_461457, whole genome shotgun sequence.
ACCESSION   DBNGZW010000734 DBNGZW010000000
VERSION     DBNGZW010000734.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1939)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1939)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1939
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_461457"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1086)
                     /locus_tag="AC16JC_17245"
     CDS             complement(<1..1086)
                     /locus_tag="AC16JC_17245"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008122329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR5166501.1"
                     /translation="MTTRCRLALAVLGATARLSAAQAYPHAAERIGTVRQVYDGVLTP
                     AIAVNTFRNIDRLFATRTIPRATRPLPLPPAARPLTRLRFVDQGRRYGLEDYLTLNRV
                     TGLLVLDHGRVALERYRQGNTRRTRWMSMSIAKSITSTLIGAAVRQGAIASLSDPVTR
                     YVPSLAGSAYEGVSIRDVLTMSSGVRWNETYTDPASDRRRLLEAQISQVPGSALAVMR
                     NLPRAAPPGTVNNYNTGETQVAAEILRNAVGRPLATYLAERIWSRVGMEADATWWLDS
                     PGGVEIGGSGFSATLRDYGRFGLFVLAGGVAGGDSILPADWMQEATTPKRLRDGTPLD
                     YGYLWWTGTTAASRRDSAFTAEGIYGQF"
     gene            complement(1098..1511)
                     /locus_tag="AC16JC_17250"
     CDS             complement(1098..1511)
                     /locus_tag="AC16JC_17250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002625855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0090729 - toxin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HIR5166502.1"
                     /translation="MPVDPEFLTEQDILLIHTELIAEFGGAQGVRDPGLLASALAQPS
                     ATSGGTFLHRGLHEMAAAYLYHIARNHAFIDGNKRTALIACLTFLDLNGVHADRREPA
                     LFDLTVAVADGRIGKDAIAERLRSYFPLPGEERTP"
     gene            complement(1501..1731)
                     /locus_tag="AC16JC_17255"
     CDS             complement(1501..1731)
                     /locus_tag="AC16JC_17255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR5166503.1"
                     /translation="MALTKKLTPMGNSMGILIDRPVLDLLGIDRDTTLEIRTDGRAII
                     IEPVKEEHIERMLTTARRVMRAQRETLKKLAG"
BASE COUNT          310 a          651 c          649 g          329 t
ORIGIN      
        1 aaactgcccg tagatcccct ccgccgtgaa cgcgctgtcc cgccgcgacg ccgccgtcgt
       61 tccggtccac cagaggtacc cgtagtcgag cggcgtcccg tcgcggagcc gcttgggggt
      121 ggtcgcttcc tgcatccagt cggcgggcag gatggaatcg cctccggcca cgccgccggc
      181 gaggacgaag aggccgaatc ggccgtagtc gcgcagcgtc gcactgaagc cgctgccgcc
      241 gatctccacc ccgccggggg aatcgagcca ccaggtggcg tccgcctcca tgccgacccg
      301 gctccagatg cgctcggcca ggtacgtcgc cagcggccgc cccaccgcat tgcgcaggat
      361 ctctgcggcg acctgcgtct ccccggtgtt gtagttgttc acggtgcccg gcggggcggc
      421 ccgggggaga ttccgcatga cggccagcgc ggatccggga acctgggaga tctgggcctc
      481 gagcaggcgg cggcggtcgg acgcggggtc ggtgtaggtc tcgttccagc ggacccccga
      541 ggacatggtg agcacgtcgc ggatggacac gccctcgtac gcactgccgg ccagggacgg
      601 cacgtagcgg gtgaccggat cggagagact cgcgatggcg ccttgccgca ccgcggctcc
      661 gatgagggtc gacgtgatcg acttggcgat cgacatcgac atccagcgcg tgcgcctggt
      721 gttcccctgg cgatagcgct cgagcgcgac gcggccgtgg tcgagcacga gcagtcccgt
      781 cacgcggttc agcgtcaggt agtcctcgag accatagcgc cggccctgat cgacgaagcg
      841 caggcgggtg agcggtctcg ctgccggggg aagtggcagc ggccgagtcg cccgaggaat
      901 cgtgcgcgtc gcgaacaacc ggtcgatgtt gcggaaggtg ttgaccgcga tcgcaggagt
      961 cagcacgccg tcatagacct gacgcaccgt cccgattcgc tccgcggcgt gcggatacgc
     1021 ttgtgcggcg gagaggcggg ccgtcgcgcc cagcaccgcg agtgccaagc ggcatcgggt
     1081 ggtcatctcg gccttgatca gggtgtgcgt tcctcgccgg ggagtgggaa gtacgatcgc
     1141 aaccgctcgg cgatggcatc cttcccgatg cgcccatccg caaccgccac cgtcagatcg
     1201 aacaaggcag gctcccgtct gtcggcatgc acgccgttca agtcgaggaa cgtcaggcag
     1261 gcgatcagcg cggtgcgttt gttgccgtcg atgaacgcgt ggttcctggc gatgtggtag
     1321 agatacgccg ccgccatctc gtgcagtccc cgatgaagga acgttcctcc ggaggtcgcc
     1381 gacggctgcg cgagcgcgga tgcaaggagc ccagggtcgc gcaccccctg ggctcctccg
     1441 aattcagcga tcagctccgt gtggatgagc aggatgtcct gctccgtcag gaattccggg
     1501 tcaaccggca agcttcttca gcgtctcgcg ttgtgcgcgc atcacccggc gcgcagtcgt
     1561 gagcatgcgc tcgatgtgct cctctttcac aggctcgatg atgatggcgc gaccatccgt
     1621 tcggatctcg agcgtagtgt ctcgatcgat tcccagcaga tcgagcaccg ggcggtcgat
     1681 caggatcccc atgctgtttc ccatcggggt cagcttcttc gtgagcgcca caggagccct
     1741 cgtgcatcca gagtactaac aatgttatat acaatgtatc taacggtcaa ttcgatgtaa
     1801 ctccttgcaa acaagccgat tacaggatta cccctaccgc agcgcggccg acgcctcctc
     1861 cagcgcggcc cgcgatgcct ccagccgcgt caccgcgtcg gccagctccc gctccgtcgt
     1921 cgccgcgtcg gcgtaccgc
//
LOCUS       DBNGZW010000735         6078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1416371, whole genome shotgun sequence.
ACCESSION   DBNGZW010000735 DBNGZW010000000
VERSION     DBNGZW010000735.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1416371"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="AC16JC_17260"
     CDS             <1..1002
                     /locus_tag="AC16JC_17260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR5166504.1"
                     /translation="LARHTEWYNVPCPQCGAAGRRETDVSDTFLDSSWYYLRYPSTEF
                     TDRPFDPARTKTWLPVTSYIGGNEHAVLHLLYSRFITMVLQQQGHVAFEEPFPKLRAH
                     GLIVKDGAKMSKSRGNVVIPDAYIKQWGADTFRMYLMFLGPFQEGGDFRDEGITGVRR
                     FLEKVWGLAHEADDALRVPEAVERKLHQTIRKVTGDTATLNYNTAIAAMMEYVNVMRE
                     HGAASRSCVRPLVLMLAPYAPHLAEELWAVLGGGDRSVFEERWPAYDERLAQAGAVEI
                     AVQVNGKLRARLQLPRGASQDDVVARALADAGVKKFVDGNPLKKIVYVQDRLVNLVV"
     gene            complement(1075..2187)
                     /locus_tag="AC16JC_17265"
     CDS             complement(1075..2187)
                     /locus_tag="AC16JC_17265"
                     /inference="COORDINATES: protein motif:HMM:NF014090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR5166505.1"
                     /translation="MPDSELKTWLHHYQDESDAAFLYSVLASVEPDARKKSTYDKLAA
                     VERRHTEMWAKLLADHGHALSAGGAAPSRTARVMAWLGRRFGPGYLLPLLLREEGREV
                     KGYIDLHRDSSGEAARAVSLTLAKESAEHAGTLAGLAGGSAEPWHQTASGGFLRNVVY
                     GFNDGLTANFGLVAGVIGASVTPHVVLISGVAGMIADALSMGASGFLAAKSEQEVYAH
                     EIAMEREEIRLMPEVEADELALIYEAKGIEEDRARQMAAQVMRDPGRALEESVREELK
                     IGEARSTPLKEGWVTGVATAVGALIPVAPFVFLSGAAAAWTAFAVAMLSHFAVGAARS
                     FFTGRSVFRSGVDMFVVGLGVAGVGYIVGDVIAKLL"
     gene            2348..2887
                     /locus_tag="AC16JC_17270"
     CDS             2348..2887
                     /locus_tag="AC16JC_17270"
                     /inference="COORDINATES: protein motif:HMM:NF019265.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5166506.1"
                     /translation="MTISLSVPDILVARAKAGDLEALEGLYRAFETPVYNLARRICRA
                     PEDAEDVVQETFLEVVRSIGHYRGEGHLWGWIRQIASSKALMRLRREKIRATDELVDE
                     AAGSPPASIGARIDLERAFERLSETSRAVVWLHDVEGFTHEEIAEQMGRTVSFSKSQL
                     ARAHARLRRLLAAEPEDGR"
     gene            2884..3462
                     /locus_tag="AC16JC_17275"
     CDS             2884..3462
                     /locus_tag="AC16JC_17275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166507.1"
                     /translation="MMHCTMDDLVAVRDGEGSVWARRHVEECATCRGEMDALFQRVAQ
                     LKALPALRPPRDRWTAVRDTIVAERTRRRRRWMRAGMALAAALAGVMIVRPMTQGPVL
                     ADELAQAKQHSAQLEDSLQLYDLDGRVMSGTQAELTARLEDQIAALDGQLAQANEQAA
                     REAQLVDLWRQRVDLMQQLVQVRVTRATYLGL"
     gene            3489..4541
                     /locus_tag="AC16JC_17280"
     CDS             3489..4541
                     /locus_tag="AC16JC_17280"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR5166508.1"
                     /translation="MTRKWIVTALAVAGIIPLALGAQETPRPSTAPRAFAYTLLSRAR
                     IGVLVNRQPNAETDKYGAKIDGVTPGGPADKAGLKAGDIITKFNGTSLAGTASEDDED
                     SGPGRKLVELVHDLDVGDTVKLEYRRGTETKTATVVTDEQQGFTFNGPVPSMREFEMP
                     RMQMPFMTAPEGRGAWNVCFGDAWCDLDLVTLNADLGDYFGTKEGVLVIKAPGDSALP
                     LKSGDVILSIGGRKPTTPAHAMRILRSYDTGESVSIEIMRHQRRQTLSYTVPEAGTMR
                     RLRPATRPRGEQSFWRTPEAAREWERVAVERQLQEALRARRLTETVRERALRSMNQRL
                     ETLRVRMQQRLNTIAI"
     gene            complement(4603..>6078)
                     /locus_tag="AC16JC_17285"
     CDS             complement(4603..>6078)
                     /locus_tag="AC16JC_17285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166509.1"
                     /translation="PRMAAHVAGLLLRNGIEVTRLTQPAAATAAHAYMGGPAGRRTFP
                     AGSYVAELAQPQRRLAKALLEPNAVLERPFVQDQVDRFRRNRRRGEDADKDEYGFYDV
                     TAWSLPYTYNVEAYWTEDAAPLGGQAVTDTLLPAPAAPGAARSAYVFPNDNLGAARLA
                     LALEQEGFNVAVALQPLVADGRTFPRGSFVVRLQRNPGSLAERIGALAPPLGVSVTPV
                     QSAFADTGNTGTGSDAVTSLAPPRILVAAGDGISETSYGWLWHFLVRDLGASFTPVAL
                     SALGTMDDLHAFNVILLPDGSGGRMRRQLGDGGVARLKDWVRSGGVLIGIGGAGDLAA
                     DKDVGFSTIATVGADSGKADSTIQAGAPPVLSPSAPPRDRPEWLPGSIFRATLDRTHW
                     LTLGYEQEQLPVYVDGSTFWKLSRKGANPLVFTGDSLTLSGFTWPDNTERLLKGTAWA
                     VVENQGDGRVVLFLGDPLFRGFWRGTARLVTNAILIGPRRR"
BASE COUNT          981 a         2046 c         2170 g          881 t
ORIGIN      
        1 ctggcgcgac acaccgagtg gtacaacgtg ccgtgcccgc agtgcggcgc cgccggccgg
       61 cgcgagacgg atgtctccga caccttcctc gactcgtcgt ggtactacct gcgctacccg
      121 tctaccgagt tcaccgaccg ccccttcgac cccgcccgca cgaagacgtg gctcccggtg
      181 accagctaca tcgggggcaa cgagcacgcg gtgctgcacc tgctctactc gcgcttcatc
      241 accatggtgc tccagcagca gggacacgtc gcgtttgagg agccgttccc gaagctccgg
      301 gcccacggcc tgatcgtgaa ggacggcgcg aagatgtcaa agtcgcgcgg caacgtggtc
      361 atccccgacg cctacatcaa gcagtggggc gccgacacgt tccgcatgta cctgatgttc
      421 ctcgggccgt tccaggaagg cggcgatttc cgcgacgagg gcatcacggg ggtgcggcgt
      481 tttctcgaga aagtctgggg actcgcccac gaagcggacg acgcgctccg cgtcccggaa
      541 gcggtggagc gcaagctgca ccagaccatc cgcaaagtca ccggcgacac ggcgacgctc
      601 aactacaaca ccgcgatcgc cgcgatgatg gagtatgtga acgtcatgcg ggagcacgga
      661 gcggcgagcc gaagttgcgt acggcccctc gtgctcatgc tcgctcccta cgcaccccac
      721 ctggccgagg agctgtgggc cgtgctgggc ggcggcgacc gttcggtgtt cgaggagcgc
      781 tggcccgcct atgacgagcg tctcgcgcag gcgggagcgg tcgagatcgc ggtgcaggtg
      841 aacggcaagc ttcgtgcgcg gctgcaactc ccccgagggg catcgcagga cgatgtcgtg
      901 gcgcgggcgc tggcggatgc cggcgtcaag aagttcgtcg acgggaatcc gctcaagaag
      961 atcgtctatg tgcaggatcg actggtgaac ctggtagtgt gaggcacagc gcacagagca
     1021 cggagcagtg cttccaggcg cgccggcagg tactgctccg tgctccctgc tccgtcagag
     1081 cagcttcgcg atcacatcgc ccacgatata cccgacgccc gccacgccca gccccacgac
     1141 gaacatgtcg acccccgagc ggaacacgct gcggccggtg aagaagctgc gggcggcccc
     1201 gaccgcgaag tgcgacagca tcgccaccgc gaacgccgtc cacgcggccg ccgctcccga
     1261 gaggaacacg aacggagcca cggggatgag cgcgcccacg gccgtcgcga cgccggtcac
     1321 ccacccctcc ttgagcggcg tgctccgggc ctcaccgatc ttcaattcct cccgcacgct
     1381 ctcctcgagg gcgcgcccgg ggtcgcgcat cacctgcgcc gccatctgac gggcccgatc
     1441 ttcctcgatg cctttcgcct cgtagatcaa cgccagctcg tccgcctcga cctccggcat
     1501 gaggcggatc tcctcccgtt ccatcgcgat ctcgtgggcg tagacctcct gctcgctctt
     1561 ggcggcgagg aatccggagg cgcccatcga cagggcgtcg gcgatcatcc ccgccacgcc
     1621 cgagatcagc acgacgtgcg gcgtgacgct ggcgccgatc accccggcca ccagcccgaa
     1681 gttggcggtg agcccgtcgt tgaagccgta caccacgttg cgcaggaacc cgcccgacgc
     1741 agtctggtgc cagggctcgg cgctgccgcc ggcgagaccg gcgagcgtgc ccgcgtgctc
     1801 ggccgactcc ttcgcgagcg tcagggacac ggcccgggcc gcttcccccg aggagtcgcg
     1861 gtgcaggtcg atgtacccct tcacctcgcg tccctcctcg cgcagcagca gcggcagcag
     1921 gtagccggga ccgaaccgtc ggcccagcca cgccatcacg cgcgcggtgc gggacggcgc
     1981 cgcgccacca gcggaaagcg catggccgtg atcggccagc agcttggccc acatctcggt
     2041 gtggcgccgc tcgacggcgg cgagtttgtc gtaggtggac ttcttgcgcg catcgggttc
     2101 gacgctcgcc aggacgctat aaaggaaggc ggcatcggat tcgtcctggt agtggtgcag
     2161 ccaggtcttg agctcggagt ccggcatggc gtgaagatac ggggctggtg gccaacttcc
     2221 tagcaggtaa tgggttacga aactgatcgg gagtggggag tggggagcgg tgggcgacca
     2281 ctccctgctc cccactctcc gcatcctgcg accatccacc accgtaccgc atctaacttt
     2341 acgaccgatg acgatctctc tgagcgtccc tgacatcctg gtagcgcgcg ccaaggccgg
     2401 agacctcgag gccctcgaag ggttgtaccg cgccttcgag acgccggtct acaacctggc
     2461 gcgccgcatc tgccgcgcgc cggaggatgc cgaggacgtg gtgcaggaga cgttcctcga
     2521 agtggtgcgg agcatcggcc attaccgggg agaggggcat ctgtgggggt ggatccggca
     2581 gatcgcgtcg tcgaaggcgc tgatgcggtt gcggcgcgag aagatccggg ccaccgacga
     2641 gctggtcgac gaggcggcgg ggtcgccgcc ggcgagcatc ggcgcgcgga tcgatctgga
     2701 acgcgcgttc gagcggctct ccgagacgtc gcgcgcagtg gtgtggctgc acgacgtgga
     2761 agggttcacg cacgaggaga tcgcggagca gatgggcagg acggtgagtt tttcgaagtc
     2821 gcagctggcc cgggcgcacg cgcgcctgcg gcgcctgctg gcggcggagc cggaggacgg
     2881 acgatgatgc attgcacgat ggatgatctg gtggcggtgc gcgatgggga gggttcggtc
     2941 tgggcgcgcc ggcacgtgga ggaatgcgcg acctgccggg gcgagatgga cgcactgttc
     3001 cagcgggtgg cgcagctcaa ggcgctcccg gcgctgcggc cgccgcgtga tcgctggacg
     3061 gcggtacgcg acaccatcgt ggcggagcgc acgcgacggc gccggcggtg gatgcgcgcg
     3121 ggcatggcgc tcgcggcggc cctcgccggg gtgatgatcg tgcggccgat gacgcagggc
     3181 ccggtgctgg ccgacgagct ggcgcaagcc aagcagcact cggcgcagct ggaggattcc
     3241 ctgcagctgt acgacctcga cgggcgagtg atgagcggca cgcaggccga gctcacggcg
     3301 cggctggagg atcagattgc cgcgctggac ggacagctcg cgcaggcgaa cgaacaagcg
     3361 gcgcgtgagg cgcagctggt ggatttgtgg cggcagcggg tggatctgat gcagcagctc
     3421 gtgcaggtgc gcgtcacccg cgccacgtac ctgggcctct gacggcccct agaatcgaag
     3481 aggaccagat gactcgcaag tggatcgtca ccgcgttggc cgtggccggg atcatcccgc
     3541 tcgcactcgg tgcgcaggag accccgcgtc cgagcaccgc gccgcgcgcc ttcgcgtaca
     3601 ctctcctcag ccgggcgcgg atcggcgtgc tcgtgaaccg ccagccgaac gccgaaacgg
     3661 acaagtacgg cgccaagatc gacggcgtca ccccgggcgg cccggccgac aaggccggcc
     3721 tcaaggcggg cgacatcatc acgaagttca acggcacgtc gctcgccggc acggcgtcag
     3781 aggatgacga agactcgggc ccgggccgca agctggtgga gctcgtgcac gacctcgatg
     3841 tcggcgacac ggtgaagctg gaataccggc gcggcaccga gaccaagacg gccaccgtgg
     3901 tgaccgacga gcagcagggg ttcaccttca acggaccggt cccgtcgatg cgcgagttcg
     3961 aaatgcccag gatgcagatg ccgttcatga ccgcgcccga agggcggggc gcctggaacg
     4021 tctgcttcgg cgacgcctgg tgcgacctcg atctcgtgac cctgaacgcc gatctgggcg
     4081 actacttcgg gacgaaagag ggtgtcctgg tgatcaaggc cccgggcgac tcggcgctcc
     4141 ccctgaagag cggcgacgtg atcctctcga tcggcgggcg caagccgacg acaccggcgc
     4201 acgcgatgcg gatcctgcgc tcctacgaca cgggggagtc ggtgtcgatc gagatcatgc
     4261 ggcatcagcg gcgccagacg ctgtcgtaca ccgtgccgga agcgggcacg atgcggcgcc
     4321 tgcgtcccgc cacgcgccct cgcggtgagc agagcttctg gcgtacgccc gaggcggccc
     4381 gcgagtggga gcgggtggct gtggagcggc agctgcagga ggccctgcgg gcgcggcggc
     4441 tgaccgagac ggtacgcgag cgagcgttgc ggtcgatgaa ccagcggctg gagacgcttc
     4501 gtgtgaggat gcagcagcgg ctgaacacca tcgcgatctg acggagaaga aagcggagca
     4561 gggagcacgg agcagtctgc tctgtgctcc ctgctctggc ttctagcgtc tccgcggccc
     4621 gattagaatc gcattcgtca ccaggcgcgc cgtcccgcgc cagaaccctc gaaacagcgg
     4681 atcaccgaga aacagcacta cccgcccgtc accctggttt tccactaccg cccaggccgt
     4741 tcccttgagc aggcgctcgg tgttgtccgg ccaggtgaac cccgagaggg tcaaggagtc
     4801 gccggtgaag acgagagggt tggcgccctt gcgcgacagt ttccagaaag tcgagccgtc
     4861 cacgtagacg ggcagctgct cctgctcgta tcccagcgtg agccagtgcg tgcggtcgag
     4921 cgtggcccgg aagatcgatc ccgggagcca ctcgggccgg tcgcgtggcg gtgccgaggg
     4981 cgagagcacc ggcggcgcac cggcctggat cgtgctgtcg gccttgccgc tgtcggcgcc
     5041 cacggtcgcg atggtcgaga accccacgtc cttgtcggcc gcgaggtctc cggcgcctcc
     5101 gatgccgatg agcacgccgc ccgagcgcac ccagtccttc agcctggcga cacccccgtc
     5161 ccccagctgc cgccgcatcc gcccccccga accgtcgggg agcaggatca cgttgaaggc
     5221 gtggaggtcg tccatggtgc cgagcgcgct cagggcgacc ggcgtgaagc tcgcccccag
     5281 gtcgcgcacc aggaagtgcc acagccagcc gtagctcgtc tcggagatgc cgtctcccgc
     5341 ggccacgagg atacggggcg gtgcgagact cgtcaccgcg tcggagccgg ttcccgtatt
     5401 cccggtgtcg gcgaacgcgc tctgaaccgg cgtgacggac acgcccaggg gcggggcgag
     5461 ggcgccgatg cgctccgcga gtgagccggg attgcgctgc agccgcacga cgaagctgcc
     5521 gcgcgggaag gtgcggccgt cggcgaccag cggctggagc gccaccgcga cattgaagcc
     5581 ttcctgttcg agcgcgagcg cgagccgtgc ggcgccgagg ttgtcgttgg gaaacacgta
     5641 cgcggaccgg gccgcgccgg gcgccgctgg cgcggggagc agcgtatcgg tcaccgcctg
     5701 gccgcccagc ggggcggcgt cctcggtcca gtacgcctcc acgttgtacg tgtaggggag
     5761 tgaccaggcg gtgacgtcgt agaagccgta ttcgtccttg tcggcgtcct caccgcggcg
     5821 gcgattgcgg cggaaccggt cgacctgatc ctggacgaat ggccgctcca gcacggcgtt
     5881 gggctcgagc agcgccttgg cgagccggcg ctgcggctgc gcgagctccg ccacgtagct
     5941 ccccgcgggg aaggtgcgcc gtcccgcggg gccgcccatg taggcgtggg ccgcggtcgc
     6001 ggcggccggc tgcgtgaggc gtgtgacctc gatcccgttg cgcaacagca gcccggcgac
     6061 gtgcgccgcc atgcgggg
//
LOCUS       DBNGZW010000736         2364 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1825044, whole genome shotgun sequence.
ACCESSION   DBNGZW010000736 DBNGZW010000000
VERSION     DBNGZW010000736.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2364)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1825044"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..284)
                     /locus_tag="AC16JC_17290"
     CDS             complement(111..284)
                     /locus_tag="AC16JC_17290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166510.1"
                     /translation="MTPHLGVFELKVWAAATGFTTGLAGMALERRWLVLVGVGLLGVA
                     FLLRFVDRRRQDP"
     gene            complement(281..1039)
                     /locus_tag="AC16JC_17295"
     CDS             complement(281..1039)
                     /locus_tag="AC16JC_17295"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR5166511.1"
                     /translation="MSERVLTTCDDGVLTGTLNRPEKKNAIDAAMIDGLYALLERADL
                     DATVRVVALRGAGKDFCAGMDLAELLDSADRSVQENREAALRFGGLFLRMRAIPKPVV
                     AVVTGRALAGGCGLATACDLVLAAESARFGYPEVQRGFVPALVMNMLRRAVGEKVAFD
                     LAATGRLLDARDAERAGLVSRVWSDEAFEEQAARLLRDLAGFSATALALTKQQLYRLD
                     GAGFEEGMRLSADVNALSRTTSDFRAAIAAFLKK"
     gene            complement(1036..1395)
                     /locus_tag="AC16JC_17300"
     CDS             complement(1036..1395)
                     /locus_tag="AC16JC_17300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtuA-related protein"
                     /protein_id="HIR5166512.1"
                     /translation="MRRTRRGVRRRPRGRRIRLVDIAHARSGDKGDTANVGLIALRAA
                     DYPLLERYVTAARVARHFKGMIRGPVERFELPNLHALNFLLHGALDGGGTISLKTDAQ
                     GKVFSTALLRMEIEVRP"
     gene            complement(1371..>2364)
                     /locus_tag="AC16JC_17305"
     CDS             complement(1371..>2364)
                     /locus_tag="AC16JC_17305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HIR5166513.1"
                     /translation="AAGHELRNLDTGRPLRDVRDRVQSVNAYLGAGPVVEALRRGADI
                     VITGRVTDKGLTLGPMMHEFGWAPDAWDLIAAGTVAGHTIECGAQCSGGNCLVDWQRI
                     PDLANVGYPIAEAHSDGTVDITKHPGTGGRVTVAGVTEQLVYEMGDPRTYITPDGIAD
                     FTTIRLKQMGRDRVRLSGIRGRPATDSLKVSAAYFYGYKAVGTLVYAWPDALKKAKAA
                     DRILRQRLDDLGLRFETVLTEYVGVDATHGPLGAGAVAAADVPEVQLRIGVRAPDRAP
                     VERFTREIAPLILNGPPSVTGFAGGRPKPEEIVAYWPALIDKTQVQPCVELVAV"
BASE COUNT          357 a          876 c          787 g          344 t
ORIGIN      
        1 cacgctgccg tcagcatacg ccctccaact cgaacgggac cctgccgtca gcggatttat
       61 ctggaaaaaa cgctgcaaat cctgcgccgg gcaggcggag ggaggcctcg tcagggatcc
      121 tgacgcctgc gatccacaaa tcgaaggagg aacgccacgc cgagcaggcc cacaccgacc
      181 aggacgagcc agcggcgctc cagcgccata ccggcgagac ccgtcgtgaa gccggtggcc
      241 gccgcccaga ccttgagctc gaacacccca aggtgtggcg tcacttcttc agaaacgccg
      301 caatcgcggc ccggaaatcg gacgtcgtgc gactcagcgc gttgacgtcc gcggacaacc
      361 gcatcccctc ctcgaacccg gcgccgtcga gccgatacag ctgttgcttg gtgagcgcca
      421 acgcggtggc gctgaacccg gccaggtcgc gcagcagccg ggccgcctgc tcctcgaacg
      481 cctcgtcgct ccacacccgc gagacgagtc cggcccgctc cgcgtccctc gcgtccagga
      541 gacggccggt cgcggccagg tcgaacgcga ccttttcacc caccgcgcgg cgcagcatgt
      601 tcatgacgag cgccgggacg aagccgcgct gcacctcggg atatccgaag cgggcggact
      661 cggccgccag caccaggtcg caggccgtgg ccagaccgca cccccccgcc agcgcccgcc
      721 cggtcacgac cgcaaccacc ggcttgggaa tggcccgcat gcgcaggaac agccccccga
      781 atcgcagcgc cgcttcccgg ttctcctgca ccgatcggtc ggcggagtcg aggagctccg
      841 cgaggtccat cccggcgcag aagtccttcc ccgccccccg cagtgccacc acgcgcaccg
      901 tggcgtcgag atcggctcgc tcgagcagcg cgtacaggcc gtcgatcatc gcggcgtcga
      961 tggcgttctt cttctccggc cggttcagcg tgccggtcag cacgccgtcg tcgcaggtgg
     1021 tcagcacgcg ctcactcacg gccggacctc gatctccatg cggagcagcg cggtggagaa
     1081 caccttgccc tgggcgtcgg tcttgagcga gatcgtgccg ccgccgtcca gcgcgccgtg
     1141 cagcaggaaa ttgagcgcgt gcaggttggg cagctcgaac cgctcgaccg gtccgcggat
     1201 cattcccttg aagtggcgcg ccacgcgggc cgcggtgacg tagcgctcca gcagcgggta
     1261 gtcggcggcg cgcagcgcga tcaggcccac gttggccgtg tcgcccttgt cgcccgaccg
     1321 ggcgtgcgcg atgtccacga gccggatgcg ccgcccgcgc gggcggcgcc tcacaccgcg
     1381 acgagttcga cgcatggctg cacctgtgtc ttgtcgatca gcgccggcca gtacgccacg
     1441 atctcctccg gcttgggccg gccgcccgcg aatcccgtga cgctgggcgg gccgttgaga
     1501 atcagcggcg cgatctcgcg cgtgaatcgc tccaccggcg cccggtccgg cgcccgcacc
     1561 ccgatgcgca gctgcacctc cgggacatcg gccgctgcga cggcgcctgc ccccagcgga
     1621 ccgtgcgtcg catccacacc cacgtattcg gtgagcaccg tttcgaatcg gagccccagg
     1681 tcgtcgaggc gctgccgcag gatgcggtcc gccgccttcg ccttcttgag cgcatcgggc
     1741 catgcgtaca ccagcgtgcc caccgccttg tagccgtaga agtacgcggc cgacaccttg
     1801 agcgagtcgg tggccgggcg gccgcggatc cccgacagcc gcacccggtc tcggcccatc
     1861 tgtttcaggc ggatggtcgt gaaatcggcg atgccgtcgg gcgtgatgta ggtccgggga
     1921 tcgcccatct cgtagaccag ctgctcggtg acccccgcga ccgtgacgcg cccgcccgtg
     1981 ccgggatgct tggtgatgtc cacggtcccg tcggagtggg cttcggcgat cggatatccc
     2041 acgttcgcca ggtcgggaat gcgctgccag tccacgagac agttgccgcc cgaacactgg
     2101 gcgccgcatt cgatcgtatg gccggcgacg gttccggccg cgatgaggtc ccaggcgtcc
     2161 ggcgcccagc cgaattcgtg catcatcggg cccagcgtga gacccttgtc ggtgacccga
     2221 ccggtgatga cgatatccgc gccgcggcgc agggcctcga cgaccgggcc ggcgccgagg
     2281 taggcgttga ccgattgcac gcggtcgcgc acgtcgcgga gcgggcgccc ggtgtcgagg
     2341 ttgcgcagct cgtgtcccgc cgcg
//
LOCUS       DBNGZW010000737         3034 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1756981, whole genome shotgun sequence.
ACCESSION   DBNGZW010000737 DBNGZW010000000
VERSION     DBNGZW010000737.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3034)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3034)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3034
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1756981"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..788)
                     /locus_tag="AC16JC_17310"
     CDS             complement(33..788)
                     /locus_tag="AC16JC_17310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HIR5166514.1"
                     /translation="MRVVRWVLPLTLVACAHRVRPGAALAPEQVLAQARAEFRAGKFT
                     KALQTLRRVQFELPPNDPALPEVHYSIAESEFQAGQLVDAAHDFRQVADQYPGSAYAA
                     AALLRAGDANMRLWRNPELDPSYGEAALAIYQDLAGRYPDSDASARAQIHVRDLRNWF
                     ADKAYKNGLFYFKRRAWDSGIIYFKDIVANYPEATRAPDALLRLVETYRVIGYKEELQ
                     EACAHLRRFYPQAGGIAKSCPAERAAPPAPGSP"
     gene            complement(794..1708)
                     /locus_tag="AC16JC_17315"
     CDS             complement(794..1708)
                     /locus_tag="AC16JC_17315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166515.1"
                     /translation="MSVRATAAALVALACAPSAADHVVLGDRNYVAGTYADALAEYQL
                     GLRAGGAGADLHAKAGAAALHTEDFSFAAQEYRALAKEDPSRAEEAVDGLERAARGAV
                     AANDRIAMAQALQGIREISPSRPLGAYARIVALDAAAAGNTADALAYLPTAIATAPDA
                     RTADSLLYVLGEAAALAKDCVMAMAVFEGVLQRQRQPVVQDAAREGIARCALMRGQSA
                     LAAGRPVDAEEWFRRAATPGSAIEVTRAAYVGLGDVALARGDVAAAVDFYQQSLMGGV
                     PGDSLAQRAQEKLNALGRADSASVPPQP"
     gene            complement(1705..2466)
                     /locus_tag="AC16JC_17320"
     CDS             complement(1705..2466)
                     /locus_tag="AC16JC_17320"
                     /inference="COORDINATES: protein motif:HMM:NF024299.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5166516.1"
                     /translation="MDASPERRVDQARERFQLQDYYGAIHLLEEISAVGHAFADAEHM
                     LGLCYSLVGQPEKALAHLDRALELNPHYVEALVHRALVLNDLGREQEATDSLRRAGQI
                     GGKSRLGFPTHLAAKLANLHAHLAEAYIEAGGMREAIGQYQAALALGPEFHDLRYKLG
                     RLLLQAGRVLEAREQFEIIVRSRPNFLDAAAMLGLASYLSGDGLSARVVWEDCRRRRP
                     EDPRVEAYLAMLDRGDLIGMPGEPAPRLSGRQRTP"
     gene            complement(2450..>3034)
                     /locus_tag="AC16JC_17325"
     CDS             complement(2450..>3034)
                     /locus_tag="AC16JC_17325"
                     /inference="COORDINATES: protein motif:HMM:NF027681.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR5166517.1"
                     /translation="PAPFSEDARADHPISPYAASKRAGELVCRSFSHLYATRIACLRF
                     FTVYGPRQRPDLAVHRFTARVLAGQPIEMRGDGSSERDYTYVSDVVDGTLAALDWTAR
                     TGRGGDGAEPWRVFNLGGGERVRLDRLITLIAEAAGCEAVIERSPDSPGDVALTSADL
                     TRSERELGYRPKVGIEEGIRRFVSWYREVHGRKS"
BASE COUNT          467 a         1101 c         1037 g          429 t
ORIGIN      
        1 cgaacgatcc gccgaacaac gcgtccactc gctcagggag aaccgggcgc gggaggagcg
       61 gcgcgttccg ccgggcagga cttggcgatg ccccccgcct gcggatagaa gcggcgcagg
      121 tgggcacacg cctcctgcag ctcttccttg tagccgatca cgcggtaggt ttctacgagc
      181 cgcagcaggg catcgggggc ccgcgtcgct tccgggtagt tggccacgat gtccttgaaa
      241 tagatgatgc cggagtccca agcgcgccgc ttgaagtaga acaggccgtt cttgtacgcc
      301 ttgtcggcga accaattgcg caagtcgcgc acgtggatct gcgcccgggc ggacgcatcg
      361 gaatccgggt accggcccgc gaggtcctgg tagatggcca gggcggcttc gccgtaactc
      421 ggatcgagct cggggttgcg ccacagccgc atgttcgcgt ccccggcgcg cagcaacgcc
      481 gccgccgcat acgcgctgcc cggatactga tcggccacct ggcggaaatc atgcgcggcg
      541 tccaccagct gccccgcctg gaattccgat tccgcgatcg aatagtgcac ctcgggcagg
      601 gccggatcgt tgggcggcag ctcgaactgc acacggcgca gggtctgcaa cgcttttgtg
      661 aacttgccgg cgcgaaactc ggcccgcgcc tgtgccagca cctgctcggg ggcgagcgcc
      721 gcgccggggc gcacgcggtg agcgcacgcc accaatgtca gaggaagcac ccaacggact
      781 acgcgcatgg acgtcacggt tgcgggggta ccgaagcgct gtccgccctg cccagcgcgt
      841 tgagtttttc ctgggcgcgc tgggccagcg aatcgcccgg cacaccgccc atcagggact
      901 gctgatagaa gtcgaccgcg gccgcgacgt cgccgcgggc cagggccaca tcgcccaggc
      961 cgacgtacgc cgcccgcgtc acctcgatcg ccgacccggg cgtcgcggcg cgccggaacc
     1021 attcctcggc atcgaccggg cggccggccg cgagcgccga ctggccgcgc atcaacgcgc
     1081 agcgtgcaat cccctcgcgg gccgcatcct ggacgaccgg ctggcgctgg cgctgcagca
     1141 ctccttcgaa gaccgccatg gccatgacgc agtccttggc gagggcggcc gcttccccca
     1201 gtacgtacag cagcgaatcg gccgtacggg catcgggcgc agtggcgatc gccgtgggca
     1261 agtacgccag ggcgtccgcc gtgttcccgg ccgcggccgc gtcgagcgcg acgatgcggg
     1321 cgtaggcgcc gagcggacgg ctcggtgaga tctcccgtat cccttgcagc gcctgggcca
     1381 tggcgatccg gtcattggct gcgacggcgc cccgtgccgc gcgctccagg ccatcgaccg
     1441 cttcctcggc gcggctcgga tcctccttgg ccagggcgcg atactcctgc gccgcgaagc
     1501 tgaaatcctc cgtgtgcagc gccgccgcgc cggctttggc atggaggtcc gccccggcgc
     1561 cgccggcgcg caggcccagt tgatactcgg ccagggcgtc ggcataggtg cccgcgacgt
     1621 aattgcggtc tccgagcacc acatggtccg ccgcactcgg cgcgcaggcc agcgccacga
     1681 gcgcggcggc cgtcgcccgg acgctcacgg agtgcgttga cgacccgaga gtcgcggcgc
     1741 cggttccccc ggcatcccga tgaggtctcc acggtccagc atggcgaggt aggcttcgac
     1801 gcgcgggtcc tccggccgac ggcggcgaca gtcctcccat acgacgcgcg ccgagagacc
     1861 gtcaccgctg aggtaggacg ccaacccgag catcgccgcg gcatcgagga agttgggccg
     1921 ggagcgcacg atgatctcga actgctcgcg ggcttcgagc acgcgccccg cctgcagcag
     1981 cagccggccc agcttgtaac gcagatcgtg gaactcgggg ccgagcgcga gcgccgcctg
     2041 atactgcccg atcgcctcgc gcatgccccc cgcctcgatg taggcctcgg ccaggtgcgc
     2101 gtgcaggttc gcgagcttcg ccgccaggtg cgtcggaaag ccgagccggg acttcccgcc
     2161 gatctggccg gcgcggcgca gcgagtcggt cgcctcctgc tcccgtccca gatcgttgag
     2221 gaccagggcg cggtgcacga gcgcttcgac gtaatgcggg ttcagctcga gcgcacgatc
     2281 caggtgggcc agtgccttct ccggctggcc cacgagcgag tagcacaggc ccagcatgtg
     2341 ctcggcatcg gcgaacgcgt gcccgaccgc cgagatctcc tccagcaggt ggatcgcgcc
     2401 gtagtagtcc tgcagctgaa agcgctcgcg ggcctgatcg acccgacgtt caggacttgc
     2461 gtccatggac ctcccggtac cacgacacga accgccgaat gccttcctcg atccccacct
     2521 tgggccggta cccgagctcg cgctcggagc gggtcagatc ggccgacgtg agcgccacgt
     2581 caccgggcga atcggggctc cgctcgatca cggcctcgca tccggcggcc tccgcaatca
     2641 gcgtgatcag ccggtcgagc cgcacgcgct cgccaccgcc gagattgaat acccgccatg
     2701 gctcggcgcc gtccccgccg cggccggtcc gcgccgtcca gtcgagcgcc gccagggtcc
     2761 cgtccaccac atcggaaaca tacgtgtaat cgcgctcact cgaaccgtcc ccccgcatct
     2821 caatcggctg cccggccagg acgcgagccg tgaaccggtg gaccgccagg tcggggcgct
     2881 gccggggtcc gtaaaccgtg aagaaccgca ggcaggcgat gcgggtcgcg tacagatgcg
     2941 agaaggaccg gcacacgagc tcccccgccc gcttgctcgc ggcgtacggg gagatcggat
     3001 ggtcggcgcg ggcatcctcc gaaaacggcg cggg
//
LOCUS       DBNGZW010000738         3230 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1825662, whole genome shotgun sequence.
ACCESSION   DBNGZW010000738 DBNGZW010000000
VERSION     DBNGZW010000738.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3230
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1825662"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1222
                     /gene="bshA"
                     /locus_tag="AC16JC_17330"
     CDS             92..1222
                     /gene="bshA"
                     /locus_tag="AC16JC_17330"
                     /inference="COORDINATES: protein motif:HMM:TIGR03999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HIR5166518.1"
                     /translation="MDGDTLRVGITCYPSVGGSGVLATALGEDLAARGHDVHFIAYER
                     PFRLPSDAPRLHFHPVAINEYELFRFPDYTLPLSVRMAAVSREHRLDILHVHYAVPHA
                     TAAILARAMLPPGQQPRVVTTLHGTDTTLLGHDAGYAPAIHHALASSDAVTAVSESLK
                     RETRRVLDFDGPIEVIHNFFAPRPPGRAPDSVRRELGLGDEVLVVHSSNLRHGKRVDL
                     LLETVARVRPREAFKLLILAGEAFAPFAADVRRLGLADRVIVREKVHAIEDYLQIADI
                     GLYTSDAESFCLSILEAMCFGCPSVSTRVGGIPEVVEDKDSGLLVPAGDVAALTQALE
                     TLIRDAPLRRALGRAAQARARERFSAEVIVPRYEALYRRLCR"
     gene            1302..1841
                     /locus_tag="AC16JC_17335"
     CDS             1302..1841
                     /locus_tag="AC16JC_17335"
                     /inference="COORDINATES: protein motif:HMM:NF017015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HIR5166519.1"
                     /translation="MKTLTYACVAFFAVVAAAVPSLLTAQTPAPSLAKELAATFQRAV
                     TEILDVAEVMPAEKYGFKPTPEISSFGDQLVHVAGITQRFIDAARGTKTEAAHHGAMG
                     KPETIDLLKKTFQSAQDTISPLTDAQLLEPVKFPFGDRTVTRATFWQGPLFQIRNHYG
                     QLVVYLRMNGIVPPTSARR"
     gene            2062..2808
                     /gene="fabG"
                     /locus_tag="AC16JC_17340"
     CDS             2062..2808
                     /gene="fabG"
                     /locus_tag="AC16JC_17340"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012121729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HIR5166520.1"
                     /translation="MVATLSGKVALVTGASRGIGRACAVKLGALGARVAVNYNTSAAA
                     ADDVVARIKAAGGEATAAQGDVSDFATAQAVVKTAIAAYGRLDILVNNAGTTRDGLLA
                     TMTEEDFDAIIRQNLKSTFNCSRAVLRQMLKQRYGRIVNITSLVGVIGGAGQTNYAAA
                     KAGIIGFTKAMAKEYGGRNIAVNAVAPGFIPTDLTSGLPAEITEHLVRLTAHGRAGTP
                     EEVANAVAFLASDEASYITGQVLAVDGGVA"
     gene            2809..>3230
                     /locus_tag="AC16JC_17345"
     CDS             2809..>3230
                     /locus_tag="AC16JC_17345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166521.1"
                     /translation="MPPLGVALARAHPGPALRFGVDTFAFRNDSRIHHKGKPDLYASR
                     CFVLGRAVVQFQRFAHFEPEAPRLAAEEYTGLVQRITRRAPWRAPLPRAERVVIPGFE
                     SLHELTRVAEAAVKAGLPRRLWTILHWTNWRIIFPRPSA"
BASE COUNT          496 a         1182 c         1075 g          477 t
ORIGIN      
        1 acgtgcggcg ggaggacatc gagagatcgg atgaagcttt cacggacctc tcgggctgac
       61 atgcggcggg tcggtagggg cggtctctga gatggatggg gatacgctgc gcgtcggcat
      121 cacgtgctac ccgtcggtcg gggggagcgg ggtgctcgcg accgcgctcg gcgaggacct
      181 ggcggcgcgc gggcacgacg tgcacttcat cgcctacgag cgtccgttcc gcctgccctc
      241 ggacgcaccg cgcctgcact tccatcccgt cgccatcaac gaatacgagc tattccggtt
      301 ccccgactac accctgccgc tctccgtgcg catggcggcg gtgagtcgcg agcatcgcct
      361 cgacatcctg cacgtccact acgccgtgcc gcacgccacc gccgcgatcc tcgcgcgcgc
      421 gatgctcccg cccggccagc agccccgcgt cgtcaccacc ctgcacggca cggacacgac
      481 gctgctcggc cacgacgccg gctacgcgcc cgccatccac cacgcgctcg cctcgtcgga
      541 cgcggtcacc gcggtgtccg agtcgctcaa gcgcgaaacc cggcgcgtgc tcgacttcga
      601 cggcccgatc gaggtcatcc acaacttctt cgcgccccgc ccgcccggcc gcgcgcccga
      661 cagtgtccgc cgtgagctcg gcctcggcga cgaagtgctc gtggtgcact cgtccaattt
      721 gcggcacggc aagcgcgtcg acctcctgct ggagacggtc gcacgcgtgc gcccgcgcga
      781 ggccttcaag ctcctcatcc tagccggcga ggcgttcgcg ccgtttgccg ctgacgtgcg
      841 gcgcctcggt ctcgccgacc gcgtgatcgt ccgcgagaag gtccacgcca tcgaggatta
      901 tctccagatc gccgacattg gcctctacac ctccgacgcc gagagcttct gcctcagcat
      961 cctcgaagcc atgtgcttcg gctgccctag cgtctccacg cgcgtgggcg gcatcccgga
     1021 ggtggtggag gacaaggaca gcggcctgct cgtgccggcg ggcgatgtcg ccgcgctgac
     1081 ccaggccctg gaaaccctga tccgcgacgc gccgctacgc cgggccctcg ggcgcgcggc
     1141 gcaggcgcgc gcgcgcgagc ggttctccgc cgaggtcatc gtgccgcgct acgaggcgct
     1201 ctaccgccgg ttgtgccggt gacggccccc gcatgcgcgc gtgggccggt caccttgacc
     1261 aggagtgaac ggtgtgggat gaatctcgct gggaggcgac catgaaaact ctgacctacg
     1321 catgcgtggc attctttgcc gtcgtcgcgg ccgccgtgcc ctcgttgctc acggcccaga
     1381 cgccggcgcc cagcctggcc aaggagctcg cggcgacctt ccagcgcgcc gtcaccgaaa
     1441 tcctcgacgt cgcggaagtg atgcccgccg agaagtacgg cttcaagccg acgcccgaga
     1501 tctcgagctt cggcgaccag ctcgtgcacg tcgccgggat cacccagcgc ttcatcgacg
     1561 ccgccagggg caccaagacg gaagccgcgc atcacggcgc gatgggaaag cccgagacca
     1621 tcgatctgct gaagaagact ttccagagcg cgcaggacac gatctcgcct ctgaccgatg
     1681 cccagctcct ggagccggtg aagttcccgt tcggcgaccg cacggtcacc cgggccacat
     1741 tctggcaggg cccgctgttc cagatccgca accactacgg ccagctcgtg gtctatctgc
     1801 ggatgaacgg gatcgtgccg ccgacgagcg cgcggcggta ggcttcgcgt ccctcacccg
     1861 gcgaaatcgc agcccatacg ccatacgcca gccgctcagg gtaagttccc cgggaatgga
     1921 cggacgcgtc ttcgttggcg ccctgagcgg cggcctcctc gccgtgccgc tcgccactga
     1981 ccacccccgc gcccgaggac agtaggccaa cctttcgatg ccgcaacctt cgcccgactc
     2041 ggcgagtgtg ctagtttcgg catcgtggcg accctcagcg gaaaggtggc gctggtgacc
     2101 ggcgcctcac gtggcatcgg ccgggcgtgc gcggtgaagc tgggcgcgct gggggcgcgc
     2161 gtcgcggtca actacaacac cagcgccgcc gcagccgatg acgtcgtcgc ccgcatcaag
     2221 gcagccgggg gcgaggccac ggccgcgcag ggcgacgtga gcgacttcgc caccgcgcag
     2281 gccgtcgtca agaccgccat cgccgcctac ggccggctcg acatcctcgt gaacaacgcg
     2341 ggaacgaccc gcgatggcct gctcgcgacg atgacggagg aggacttcga cgccatcatc
     2401 cggcagaacc tcaagagcac gttcaactgc agccgcgccg tcctccgaca gatgctgaag
     2461 cagcgatacg gccgcatcgt caacatcacg tcgctcgtgg gagtgatcgg cggcgcgggc
     2521 cagacgaact acgcggccgc gaaggccggg atcatcggct tcacgaaggc gatggcgaag
     2581 gagtacggcg gacgcaacat cgcggtcaac gccgtcgcgc ccggtttcat ccccaccgac
     2641 ctgacgagcg gtctgccggc ggagatcacg gagcacctcg tcaggctgac agcccatggg
     2701 cgggcgggga ccccggaaga ggtggccaac gcggtggcgt tcctcgcatc cgacgaggcc
     2761 agctacatca ccgggcaggt cctggccgtc gacggcggcg tggcgtaagt gccgcccctg
     2821 ggcgtcgccc tggcccgtgc gcatccgggc cctgccctcc ggttcggggt ggacacgttc
     2881 gcgtttcgga acgacagccg gatccaccac aagggcaagc ccgatctcta cgccagccgg
     2941 tgcttcgtgc tggggcgggc cgtcgtgcag ttccagcgct tcgcccattt cgagccggag
     3001 gccccgcgcc tcgccgcgga ggagtacacc gggctggttc agcgcatcac gcgccgcgcc
     3061 ccctggcgcg cgccgctacc gagggccgag cgggtcgtca tccccgggtt cgagtcgctg
     3121 cacgagctga cgcgcgtggc ggaggccgcg gtgaaggcgg ggcttccgag gcggctctgg
     3181 acgatccttc actggacgaa ctggcgcatc atcttcccgc gccccagcgc
//
LOCUS       DBNGZW010000739         1770 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1418163, whole genome shotgun sequence.
ACCESSION   DBNGZW010000739 DBNGZW010000000
VERSION     DBNGZW010000739.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1770)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1770)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1770
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1418163"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /locus_tag="AC16JC_17350"
     CDS             complement(<1..270)
                     /locus_tag="AC16JC_17350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018412777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166522.1"
                     /translation="MNFQFHFSRKAKLITAAVIAAPILAFSIYTWSALNYSYSAGERG
                     PGYVQKFSRKGWLCKTWEGEFALLPLPGTFSPERFEFTVRDDVIAQ"
     gene            complement(267..782)
                     /locus_tag="AC16JC_17355"
     CDS             complement(267..782)
                     /locus_tag="AC16JC_17355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5166523.1"
                     /translation="MRVTRTYLQLSAPGQFRAAFGDFPDFRLERVLHPSPALYRQCYR
                     TVGEAHRWRDRWDWSDAEIRAHLADPNITFHVATRAGALAGYYELRRVADDDSVEVAY
                     FGLVPEALGRGLGKHLLSCAVRDAWALIPPPKRVWLHTCTLDHPNALPNYMARGFVPY
                     KTEQYQVDSAT"
     gene            836..1204
                     /locus_tag="AC16JC_17360"
     CDS             836..1204
                     /locus_tag="AC16JC_17360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166524.1"
                     /translation="MTSPLHRAGYHTVPLPLALHLAGHTVTGLFCMFAGGLNLIDPGH
                     ILRLGLPLLALTGFSWGYVFGILMGRKEVLALGFLASIAYAAVGVLKFGSDRPFGLLL
                     IAIGVYGLAVLTIYRNRIVD"
     gene            complement(1209..>1770)
                     /locus_tag="AC16JC_17365"
     CDS             complement(1209..>1770)
                     /locus_tag="AC16JC_17365"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR5166525.1"
                     /translation="AGIDAIGRPCDVSDPAAVAAFAGFVDRERGDAAVLVNNAGVGRF
                     APLAELTLEDWDLVMGVNVRSLYLMTRAFLPGLARTGASAIVNIASLAGKNGIEGGTA
                     YCASKHAVLGFSKALMLEVRKRGIRVVAVCPGSVSTPFMDKQSRLRPNRDRALQADDV
                     AQAVLGALTLPDRAMLSELDLRPTNP"
BASE COUNT          292 a          596 c          592 g          290 t
ORIGIN      
        1 ctgggcgatg acgtcgtcac gcacggtgaa ttcgaaccgc tccgggctga acgttccggg
       61 caacggcagc agcgcgaact cgccctccca cgtcttgcag agccagcctt tcctcgaaaa
      121 cttctgcacg tagccgggtc cgcgctcacc ggcggaatag ctgtagttca gcgcagacca
      181 ggtgtagatg gagaaggcga gaatcggcgc ggcgatgacg gccgcggtga tgagcttggc
      241 cttgcgggaa aaatggaact ggaaattcat gtggcggaat ctacctggta ctgttccgtc
      301 ttataaggga cgaagccccg cgccatgtaa ttcggcagcg cattggggtg atcgagcgta
      361 caggtgtgca gccacacgcg cttgggtgga gggatcaatg cccaggcgtc gcgcacggcg
      421 cacgacagca ggtgcttgcc gagcccgcgc cccagcgcct ccggcacgag gccgaagtag
      481 gcgacctcca ccgaatcgtc gtcggccacg cggcgcagct cgtagtagcc ggccagcgcg
      541 ccggcgcggg tggcgacgtg gaacgtgatg ttcgggtcgg cgagatgcgc acggatctct
      601 gcgtcgctcc agtcccaccg gtcgcgccag cgatgcgctt cccccaccgt ccggtagcac
      661 tggcgataga gggcagggga gggatggagg acgcgctcca gccggaaatc cgggaagtcg
      721 ccgaaggcgg cccggaattg gccgggggcg ctcaactgga gatacgttcg cgtcactcgc
      781 atggggttgg cctgcgcgtt attttagcta cgtctccaac ccccgtgaat gcgacatgac
      841 gtcaccgctc caccgcgccg gctatcacac cgtgcccctc ccgctggccc tgcacctggc
      901 cggccacacg gtgaccggcc tgttctgcat gttcgcgggc gggctgaacc tcatcgatcc
      961 cggacatatc ctgcgactcg gtcttcccct gctggcgctc acggggttca gctggggata
     1021 cgtgttcggg atcctgatgg ggcggaaaga agtgctggcg ctgggtttcc tcgcgagcat
     1081 cgcctacgcc gcggtcggcg tgctcaagtt cggcagcgac cgtcccttcg ggctgttgct
     1141 catcgcgatc ggcgtgtacg ggctcgccgt gctcacgatc taccgcaacc ggatcgtcga
     1201 ctagcgtctc aggggttcgt cggccgcaga tccagctcgc tcaacatcgc gcgatccggc
     1261 agggtcagcg cgccgagcac ggcctgggcc acatcgtcgg cctgcagcgc acgatcccgg
     1321 ttcggccgca gccgcgattg cttgtccatg aagggcgtgg acaccgagcc ggggcacacc
     1381 gcgaccacgc ggatgccgcg cttgcggacc tcgagcatca gggccttgga gaatcccagc
     1441 accgcgtgct tcgaggcaca gtaggccgtg ccgccttcga tcccgttctt tcccgcgagc
     1501 gacgcgatgt tcacgatcgc gctcgcgccc gtccgcgcca ggccggggag aaacgcgcgc
     1561 gtcatgagat agagcgagcg cacgttcact cccatcacca ggtcccagtc ctccagcgtg
     1621 agctccgcga gcggggcgaa gcgcccgacc ccggcgttgt tcacgagcac tgccgcgtcc
     1681 ccgcgctcac ggtccacgaa gccggcgaac gcggcgaccg ccgcgggatc cgacacgtcg
     1741 cagggacggc cgatcgcgtc gatgccggcc
//
LOCUS       DBNGZW010000740         1665 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1014787, whole genome shotgun sequence.
ACCESSION   DBNGZW010000740 DBNGZW010000000
VERSION     DBNGZW010000740.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1665)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1014787"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            159..>1665
                     /locus_tag="AC16JC_17370"
     CDS             159..>1665
                     /locus_tag="AC16JC_17370"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:NF012936.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0070981 - L-asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B family protein"
                     /protein_id="HIR5166526.1"
                     /translation="MGAIFGIVGEGSLAEVRAMGQAVAHRGQYQQCWNPAPNVYFGRA
                     EHRPFEAGSSPMAADWHASSDEASFPERFAREGLAALANLRGTFAVAVCGGPAHIWLA
                     VDQIGYKSLYYTVLRGRFAFASEYKSLLQLADLPLEPDRTAIQHYLATKHPLGGRSFL
                     ARARTLQGGNVLELRDKRVAVSTYWKPGTDVVQRSDQEHVSVVREALLETVHRQVHAY
                     EHVGITLGGGVDAAIVLGTLRRVAPDVRISSFTIGAAPDDWEVVGAHQTADQFGTEHH
                     QFLFDPTTIPAELPQLVWLTEDCGGREEAMLQMHVLRHAGEHTNVVFGGHGADVLFGG
                     MPRHRLIGLAERLPMFSTPLQELFQLSQCGLAPSSLMGRALQTAVYGNTPPRALRVPG
                     AGSSKGVFWRPEVNEFIRATIQRMHSLNYLEPNHELTGATFHSPFLDPDMIATSLTVP
                     GWLKSGWRRQKWVLREAAADLLPMSIRHRKKAIQRLDVQGTMGEVLSDLAGN"
BASE COUNT          259 a          571 c          561 g          274 t
ORIGIN      
        1 gcgacgagtg atcttccgct ttccgggttg agctgtaatt tcggtcagtt cccaagccaa
       61 ttcgacaacc tcatcccccg cccgcctccg attcccgggt ggccggcggg ttgaccgcct
      121 gttttctgaa cgttcaacgc cggagaggta agtcgttaat gggagccatc ttcggtatag
      181 taggcgaagg gtcgctggcg gaagtcaggg ccatgggtca ggcggtggct caccggggcc
      241 aataccagca gtgctggaat ccggcaccca acgtctactt cgggcgggcg gagcatcgac
      301 ccttcgaggc cggcagctcc cccatggccg cagactggca cgcctccagc gacgaagcct
      361 ccttccccga gcgttttgcc cgggaaggac tggccgccct ggccaacctt cggggtacct
      421 tcgccgtagc ggtctgtggc ggccctgcac acatctggct cgcggtcgac cagatcggct
      481 acaagagtct gtactacacg gtgctccgcg gccggttcgc gtttgcgagc gaatacaagt
      541 cgctgctgca gctggccgac ctgccgctcg agccggaccg caccgccatc cagcactacc
      601 tcgccaccaa gcacccgctc ggtggtcgct cgttcctggc gcgcgcccgc accctgcaag
      661 gcgggaacgt gctcgagctc cgggacaagc gggtcgccgt ctcgacctac tggaagccgg
      721 ggaccgacgt cgtccagcgg tcggaccagg agcacgtctc ggtcgtgcgg gaggccctgc
      781 tcgagacggt gcaccgccag gtgcacgcct acgagcacgt gggcatcacg ctcgggggcg
      841 gcgtcgacgc ggcgatcgtg ctcggtacgc tccgccgggt cgcgccggac gtgcgcatct
      901 cgtccttcac tatcggcgcc gcgccggacg actgggaggt cgtgggcgcg caccagaccg
      961 cggaccagtt cgggaccgag catcaccagt tcctgttcga ccccacgacg attccggccg
     1021 agctgccgca actggtctgg ctcaccgagg actgcggcgg ccgcgaagag gcgatgctgc
     1081 agatgcacgt gctgcggcac gccggcgagc acaccaacgt ggtcttcggg ggtcacggcg
     1141 ccgatgtgct gttcggcggc atgccgcggc accggctcat cggcctggcc gagcggctgc
     1201 caatgttcag cactccgctg caggagctat ttcagctctc gcagtgcggt ctggcaccct
     1261 cgagcctgat gggccgcgcg ctgcagaccg ccgtgtacgg caacaccccg ccccgggcgc
     1321 ttcgggtgcc gggtgccggg tcgtcgaagg gcgtgttctg gcggccggag gtcaacgagt
     1381 tcatccgcgc gaccattcag cgcatgcact cgctcaacta tctcgagccc aaccacgagc
     1441 tgaccggcgc gaccttccac tcgccgttcc tggatcccga catgatcgcg accagcctga
     1501 cggtgcccgg ctggctcaag tcgggatggc ggcggcagaa gtgggtcctg cgggaggcgg
     1561 cggcggacct gctgccgatg agcatccggc atcggaagaa ggccatccag cggctcgacg
     1621 tgcagggcac gatgggcgaa gtgctctcgg acctggcggg caact
//
LOCUS       DBNGZW010000741         1906 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_463198, whole genome shotgun sequence.
ACCESSION   DBNGZW010000741 DBNGZW010000000
VERSION     DBNGZW010000741.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1906)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1906)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1906
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_463198"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(138..1559)
                     /locus_tag="AC16JC_17375"
     CDS             complement(138..1559)
                     /locus_tag="AC16JC_17375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR5166527.1"
                     /translation="MTTAKTAEVGRGRKAPTVVEIDGFRGRLISADDADYDTARAVWN
                     GAIERRPRLIARCIGTADVVAAVRFARDHDLEIAIRGGGHNVAGTAVCDDGIVIDLSA
                     MRAVRVDPADRRAWVQGGALWGDVNHETQVHGLATTGGIVSHTGVAGLTLGGGVGWLM
                     RKHGLTVDNLLAVDVVTADGGLLRASENEHPDLFWALRGGGGNFGVVTSFEFRLHSVG
                     PTVVAGPILWDATDVGEILRFYRDFVRDAPDELGTVVRFGTAPPLPVIPKDLHWRPVV
                     MVGTCYAGPIEDGELALAPLRASRTPLLDLVGPTPYAGFQSALDSTVVHGWNYYWKST
                     HLPELRDALIDVIAEHAFSCSSPRSYAAMFHLKGAVSRVAEGATAFGNRQASHAITLD
                     AVWRPGEDFGDRDTAWTRAFFAALGRFREGVYVNFLGGDEDPDRVREAYGDSVYDRLV
                     DVKTTYDPDNVFHHNQNIPPEVE"
     gene            1664..>1906
                     /locus_tag="AC16JC_17380"
     CDS             1664..>1906
                     /locus_tag="AC16JC_17380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011751617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HIR5166528.1"
                     /translation="MRTYGQYCPIARGAEIFAERWTPVIIRNLHVGCGSYSEILAGAP
                     GLSRTLLSQRLKQLERVGIVESAPKPDGRGRHYELTS"
BASE COUNT          325 a          638 c          632 g          311 t
ORIGIN      
        1 cattgaatca tgccatcacg cgcagttcgc gcaaggcgca tccgagctgt cgccgcagca
       61 gcgtgcgcgt ggtgacgccg tcggtagccg acatggcggc gatgcgctcg tgtgatcggt
      121 ggtctcgagc agatgcatca ctcaacctcg ggcgggatgt tctggttgtg gtggaagaca
      181 ttgtcggggt cgtacgtggt cttcacgtcc accagccggt catagacaga gtcgccgtac
      241 gcctcgcgga cccggtcggg gtcttcatcg ccgccgagga agttgacgta gacgccttcg
      301 cggaagcggc cgagggcggc gaagaacgcc ctcgtccaag cggtgtcccg gtcgccgaag
      361 tcctcgcctg gccgccacac agcatcgagc gttatcgcgt gcgatgcctg ccggttcccg
      421 aacgccgtcg cgccttcggc cactcggctc accgccccct tcagatggaa catcgccgcg
      481 tacgaccgtg gcgacgagca ggagaacgcg tgctcggcga tcacgtcgat aagggcgtca
      541 cggagctcgg ggaggtgggt ggacttccag tagtagttcc acccgtggac gacggtcgag
      601 tcgagcgcgc tctggaatcc cgcgtacggc gtgggcccga ctaggtcgag gaggggagtt
      661 cgggaggcgc gcagcggggc gagcgccagc tcaccgtcct cgatcggccc cgcgtagcag
      721 gtacccacca tgacaaccgg gcgccagtgc agatctttgg ggatgaccgg tagcgggggc
      781 gcggtgccga acctcacgac cgtgccaagc tcgtcgggag catcgcggac gaagtcgcga
      841 tagaagcgga ggatctcacc gacatcggtt gcgtcccaga ggatgggccc ggccacgacg
      901 gtgggcccga cggagtggag gcggaactcg aacgaggtga ccacgccgaa attgccgccg
      961 ccgcctcgca atgcccagaa caaatcgggg tgctcgttct cggacgcccg cagcaggccg
     1021 ccgtcggccg tcacgacatc gacggcgaga aggttgtcga ccgtgaggcc gtgcttgcgc
     1081 atcagccagc ctacgccgcc gccgagggtg agtccggcga cgccggtgtg gctcacaatt
     1141 ccgccggtgg ttgcgagacc gtgcacctgc gtctcgtggt tgacgtcccc ccacagcgca
     1201 ccaccctgca cccatgccct gcggtcggca ggatcgaccc gcacggcccg catcgccgag
     1261 aggtcgatga cgatcccatc gtcgcaaacg gcggtacccg ccacgttgtg gcctccgccc
     1321 cgtatagcga tctccagatc atgatcgcgg gcgaaacgca cggccgcaac cacatcggcg
     1381 gtcccgatgc atcgggcgat cagtcgcggg cggcgctcga tggcaccgtt ccaaactgca
     1441 cgggcggtgt cgtagtcggc gtcgtcggca ctgatcagcc ggcctcggaa cccgtcgatc
     1501 tccacgacgg ttggcgcctt gcgcccgcgg cctacttccg cggtcttcgc tgtcgtcatt
     1561 gtgtcctcct gtctcgatgc ccacatcgga ccaccgcaga ccgcggttgt atagtcaaaa
     1621 atctggactt cactaccgaa ggtgggtgcg caatactggc gtcgtgagaa cgtacggcca
     1681 atactgtccc atcgcccgtg gcgccgagat cttcgccgag cgctggacac cagtgatcat
     1741 ccgcaacctg cacgttggct gcggaagcta cagcgagatc ctggcgggcg cgccgggact
     1801 ctcccgtacc ctgctgtcac agcggctcaa acagctcgaa cgcgtcggca tcgtcgagtc
     1861 ggctcccaag cccgacgggc gcgggcgcca ctacgagctt acgtcc
//
LOCUS       DBNGZW010000742         2748 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1076291, whole genome shotgun sequence.
ACCESSION   DBNGZW010000742 DBNGZW010000000
VERSION     DBNGZW010000742.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1076291"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..>2748)
                     /locus_tag="AC16JC_17385"
     CDS             complement(250..>2748)
                     /locus_tag="AC16JC_17385"
                     /inference="COORDINATES: protein motif:HMM:NF015644.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family membrane protein"
                     /protein_id="HIR5166529.1"
                     /translation="APDQVFLRFNEGTAGLDVMELLRRLVLPTAIGLALLFGLGASAG
                     WLGVLQFLHRTPFGVADPVFGRDVGYYVFTVPVVGSVLGFLTALTLIALLGSVGLYVL
                     RRVVVLVGRRVTVEPSARWHIAGLIAFLFLLAALRAYFVRIPGLLYSTTGPLVGASFA
                     DLHAKLLGLRLAGLAALVAGALVLWGTRTQQLARATLLGLGVYLGVSLLGVAVYPTII
                     QRLVVAPNELVKETPQLAFHIAATRKAWGLDSVVTRDLTGETGLTQRDIEANQATIQN
                     VRLWDREPLLQTFRQLQEIRTYYDFVSVDDDRYWIDGQYRQVLLSPRELNAGSLPTRT
                     FINERLTFTHGMGLTLGRVNQVTPEGLPVLFLKDLPPVSTVSLRVTRPEIYFGELTDS
                     WVFANTHQREFDYPSGDENIYGSYTGQGGVRVGSWLRRAVLAAYFRSLKVLLSSDITN
                     DSRALYIRNIRERAQTALPFLFYDRDPYLVVSDSGRLRWILDAYTATDRYPYAKPLDD
                     GTNYVRNSVKVVIDAFDGTVTAYLADPADPLARTLAGVFPGIFQPLDAMPADLRAHLR
                     YPEDLFRMQTDMYATYHMADPDAFYHREDQWQKPVLSRTGQQQDPFLRHMVMRLPGER
                     QAEFIFMVPFTPTGKDNLASWMVARNDGAQYGKLIVYRFPKQSLVYGPGQIVNRISQD
                     PEISRQVSLWDQRGSEVIRGNLLVIPIEESLIYVQPIYLRAEGGGIPELKRVVVAYQN
                     QVVMEETLEAGLTELFSGAAPPATLAAETAGPSPAGRPGDNRAADLTRQAVQLYQSAV
                     AAQRAGDWAQYGTDLARLGEVLRQLQSALEGRRP"
BASE COUNT          467 a          949 c          924 g          408 t
ORIGIN      
        1 acaccagacg gcatgcttgg tcaattgacc tctcctactt ggggaacttc tggacaagta
       61 cgcgcgacgc cgggggtggg gcgcgggcgc gttcgacgcg ggggcgctcg acccgggtca
      121 atctttcgca tctcactgcc ggccatgagc aacaatgccc gggccgtgcg gccctcgcgt
      181 ccgcgccccc accccggcgt cgccccttat ccagagcttc ttcagggata tgggaatgaa
      241 ctgacgcagc tacggcctcc ggccctcgag cgccgactgc agctgccgca gcacttcgcc
      301 cagccgagca agatccgttc cgtattgcgc ccagtcaccg gcgcgctgcg ccgcaacggc
      361 gctctgatac agctggacgg cctggcgggt taggtcggca gcccgattgt cgccgggccg
      421 gccggcgggt gacggcccgg ccgtctcggc cgccagcgtg gcgggaggcg ccgcaccgga
      481 gaacagctcc gtcaacccgg cctcgagcgt ctcctccatc acgacctggt tctggtacgc
      541 gacgacgacg cgtttcagct cggggatccc tccgccctcg gcccggagat agatcggctg
      601 cacgtagatg agcgactcct cgatcgggat caccagcaga ttgccgcgga tcacctcgga
      661 gccgcgctgg tcccagagcg agacctggcg ggagatctcg ggatcctggc tgatccggtt
      721 gacgatctgg cccggcccgt agacgagact ctgcttcggg aagcgataca cgatcagctt
      781 gccgtactgc gccccgtcgt tgcgcgccac catccacgag gccaggttgt ccttgccggt
      841 cggcgtgaac gggaccatga agatgaactc cgcctgccgc tcccccggca gccgcatcac
      901 catgtggcgc aggaagggat cctgctgttg gcccgtccgc gacaataccg gcttctgcca
      961 ctggtcctcc cggtggtaga aggcatccgg gtccgccatg tggtaggtcg cgtacatgtc
     1021 ggtctgcatc cggaacagat cctcggggta gcgcaggtgc gcgcggagat cggccggcat
     1081 cgcgtcgagc ggctgaaaga tgccggggaa caccccggcc agggtgcgcg ccagcggatc
     1141 ggccggatcg gcgaggtagg ccgtgacggt gccgtcaaag gcgtcgatca ccaccttcac
     1201 gctgttgcgc acgtagttgg tgccgtcgtc gagcggcttc gcgtagggat agcggtcggt
     1261 cgcggtatag gcatcgagga tccagcgcag ccggcccgaa tccgagacca cgagataggg
     1321 gtcgcggtcg tagaacagga acggcagcgc ggtctgcgca cgctcccgga tgttccggat
     1381 gtacaacgcg cggctgtcgt tggtgatgtc gctcgacagc aacaccttca gggagcggaa
     1441 ataggccgcg agcaccgctc ggcgaagcca cgagccgacc cggactccgc cctgccccgt
     1501 gtacgacccg tagatgttct cgtcccccga gggatagtcg aactcccgct ggtgcgtgtt
     1561 ggcgaacacc caggaatcgg tgagctcgcc gaagtagatc tccgggcgcg tgacccggag
     1621 cgacaccgtg gacacgggcg gcagatcctt gaggaacagg accggcagtc cctcgggcgt
     1681 cacctggttc acccgcccca gcgtgagccc catcccgtgc gtgaaggtga gccgctcgtt
     1741 gatgaacgtc cgcgtcggca gcgagcccgc gttcagctcg cgcggtgaca gcagcacctg
     1801 gcggtactgc ccgtcgatcc agtagcggtc gtcgtccacc gagacgaagt cgtagtaggt
     1861 gcggatctcc tgcagctgcc ggaacgtctg cagcagcggc tcgcgatccc acagccggac
     1921 gttctggatg gtggcctggt tcgcttcgat gtcgcgctgg gtgagcccgg tctcgcccgt
     1981 caggtcgcgc gtcaccacgc tgtccagccc ccacgccttg cgcgtcgccg cgatgtggaa
     2041 ggcgagctgc ggcgtctcct tcaccagctc gttcggcgcc acgaccaggc gctggatgat
     2101 cgtggggtac acagcaacgc cgagcagcga tacgccgaga tagaccccga gcccgagcag
     2161 ggtggcccgg gcgagctgct gggtgcgggt cccccacagc accagcgcgc cggccaccag
     2221 cgccgccagt ccggccagcc gcaggccgag gagcttcgcg tggagatcgg cgaagctcgc
     2281 gcccaccagc ggtcccgtcg tcgagtacag cagtcccggg atgcgaacga agtacgcccg
     2341 caacgccgcc agcaggaaca ggaaggcgat caacccggct atgtgccacc gagccgacgg
     2401 ttccaccgtg acgcgacgcc cgaccagcac cacgacgcgg cgcagcacgt acagtccgac
     2461 gctcccgagc agcgcgatca gcgtcagcgc cgtgaggaag ccgaggacgc tccccacgac
     2521 cggcaccgtg aacacgtagt agccgacatc gcggccgaag accgggtccg cgacgccgaa
     2581 tggcgtccgg tgcaggaact ggagcacgcc gagccaccct gccgaggctc ccaggccgaa
     2641 cagcagcgcc agaccgatcg ccgtgggcag caccagccgg cgcaacagtt ccatcacgtc
     2701 gaggccggcg gtcccctcgt tgaagcggag gaacacctgg tctggggc
//
LOCUS       DBNGZW010000743         6439 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_123163, whole genome shotgun sequence.
ACCESSION   DBNGZW010000743 DBNGZW010000000
VERSION     DBNGZW010000743.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 6439)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6439)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6439
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_123163"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..95)
                     /locus_tag="AC16JC_17390"
     CDS             complement(<1..95)
                     /locus_tag="AC16JC_17390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HIR5166530.1"
                     /translation="MARGKVKWFNDAKGYGFIEQEGGEDVFVHFSA"
     gene            complement(210..2435)
                     /locus_tag="AC16JC_17395"
     CDS             complement(210..2435)
                     /locus_tag="AC16JC_17395"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR5166531.1"
                     /translation="MRRAVLGLLSAIWPAGIAAAGAQTSERPAQRAESLWVAGRPWHT
                     AETLLDAAARNPRQDAAFIVRSAEAELEARRFDRARSLLVGQPWLEDYGAGEALRVLG
                     EAEWRLGEPGRAAQRFVAARGRARRPEADLLAVRAAMAFEAAGLPDSAARYYAAARSG
                     GLRSIEPWLRLREARVSRDTTAARRLLVDLPPAAARGAAAARAGALLTAGDSSDARAA
                     FRDAGLTLPAIRLGLALGDTAAAAGELYALFGRAPQSDDAAAGVALAHGPLPPRSPAD
                     RVALARVLRGHGAATEARQQLTRALSEGDSSGATLILMGELLTSLGRYPDAAGAYRAA
                     ALDTAWTALALYRRGRVFVRIADPGTPQALAGFAETFPGDTAAPTALYLLGDFLTDRG
                     DEAGAERWFSELTRRYPADARSSLARFRMAAAARRRGDPDSAAYYYAAEVLVGGAQRN
                     AARYWLGRLARERGDTTRADSLWRALAREDSIGYYGLRARRAAGMESLTLAADSTAAT
                     PTATAGLALLDTLLLAGLDTEARLEVRELLARAPQPVSDLLAWSDGLSMRGWGSAGVR
                     LGWVASARGVSDARVLRAIYPWPWRAAVEAEAREFGVDPLLLAAIVRQESVFDLEALS
                     RAGARGLAQLMPGTAAQAARGLDVTLYPEWLTVPDLNLHLGAAHLATLLRRFGGRVDV
                     AVAAYNAGAAPVSRWLSLPGADDPDEFIEQIPYPETRTYVRSVLRNRDLYRALYSPTS
                     R"
     gene            complement(2435..3769)
                     /locus_tag="AC16JC_17400"
     CDS             complement(2435..3769)
                     /locus_tag="AC16JC_17400"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HIR5166532.1"
                     /translation="MTIASCTACGTWQRVGSPNTPQAGVEVARLFDAGTIYDRMGLFV
                     TGAPLPTVATIRFLAGPSPDSTLVLFALSLANHSLSFRREGNEFIAEYHVELAFRSDS
                     GAPVMLASDQQVRVRGFQETLRADESVLFQGMLSLRPGVYNASVTIRDRNSPAYARRE
                     RPDTVPRLVGPTLAGPLPFYEGSGRSSTREPPQLLLNPRATLPYGADTLRFYVEGYGL
                     PSGTRLAARAVDRNDNVLWQDTLPLQDSAGFSRAVVRVAGSELPVGEQEFVLGAVGGA
                     QQSRTPFLVSFSSQWVLTNYAEMMNLLRYFDRPDLVAKLRNAPPEQRAAAWRAFWTAT
                     DPAPLTPENEALDEYLHQIQIANFRFQEAGRPGWLTDRGEVFITLGEPDEVMDLSSPG
                     ATRSGPQSIRWSYTTLRLVLLFQDLTGFGRFELTPGSRADYLQVLARLRRQQ"
     gene            3838..4749
                     /locus_tag="AC16JC_17405"
     CDS             3838..4749
                     /locus_tag="AC16JC_17405"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5166533.1"
                     /translation="MTSENLAGRTIAGYRLLERVGEGGTAEVYRAEHAEHGACAFKVL
                     RPRLRGDPTAVKRFLREASYGARVLHPSVVRTYDYGEADGLYYLALQWAGGQSLADFL
                     HQSGPVAPAVAAGIVSSLAAGLAAAHEVGIVHRDLKPENIIHDSATGVTKLLDFGIAR
                     DAELPPEERLTRTGFFVGTLQYVAPEALSGELVDARADIYSLATIAYYMLSGRHPFSG
                     RNPRELFQQLLREAPLPLNEALPGRRFPAALEAAVMRGLSRDPAQRQPSVTSFADEFA
                     AAVAGTTEAPGSSLLGTLRRFMGGKKD"
     gene            4755..5330
                     /gene="pyrE"
                     /locus_tag="AC16JC_17410"
     CDS             4755..5330
                     /gene="pyrE"
                     /locus_tag="AC16JC_17410"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012361024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR5166534.1"
                     /translation="MSATTPPNHEDAIGRLAELLRQRSLTHGDFVLASGRRSTYDIDA
                     RKTTMSGEGQVLIGTAGLLALQSRGWHPRSVGGLTLGADPVAYAIAHSARLAGTSLDA
                     FTVRKQAKDHGTGRRIEGCLAAGDAVVVIEDVITTGRSALDAVAAARAEGANVLGVLA
                     VIDRQEGGREAIEGAGLAVVVLVTARDLGVE"
     gene            5342..>6439
                     /locus_tag="AC16JC_17415"
     CDS             5342..>6439
                     /locus_tag="AC16JC_17415"
                     /inference="COORDINATES: protein motif:HMM:NF025885.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HIR5166535.1"
                     /translation="MSDAPLVVLQLSDSFNEYWPLLARELGVPLVVRDPATDVEALPT
                     HALAMVVAAGGAETEVGPLLGRIDLPASIPLVVAGASTSHRIAAHVVAVGAADYFALP
                     EDRDALRSTLAAALERSRAAHDRIALATLEAQAHAFREIVGDSPALQAALSRAARVLP
                     HADATVLVTGETGTGKELLARALHYGGPRAAAPFVELNCAALPSALLESELFGHERGA
                     FTDAKAAKPGLFEVAAGGTLFLDEIDSLALELQSKLLRALEQKVVRRLGATTTRSLEV
                     RIVAATNSRLDRAVAEGRFREDLYYRLNVVTLELPPLRSRGDDVLRLVAHFVAKFSKQ
                     YTIPEPALTADTRRALLAHTWPGNVRELRNIV"
BASE COUNT          961 a         2320 c         2249 g          909 t
ORIGIN      
        1 gccgagaagt gcacgaacac gtcctctcca ccctcctgct cgatgaaccc gtaccccttc
       61 gcgtcgttga accacttcac ctttccgcgt gccattgcct ccaccctccc tgcgttagtc
      121 cacaggaaca acgggattga ccgctcgcac tccggcgagc gtgggcattg aaaaggcacc
      181 gggttcggac ggttgtcaag cgagccgggt caccggctcg tgggcgagta cagggcgcga
      241 tagaggtcgc ggttgcgcag caccgagcgg acgtaggtcc gggtctccgg gtagggaatc
      301 tgttcgatga actcgtcggg gtcgtcagcc cccgggaggg acagccagcg gctcaccggc
      361 gccgcaccgg cgttgtacgc cgccacggcg acgtccaccc gcccgccgaa gcgccgcaac
      421 agggtcgcga ggtgtgccgc gccgaggtgg agattgaggt cggggaccgt cagccattcc
      481 gggtacaggg tcacatccag gccgcgggcc gcctgcgcgg cggtgccggg catcagctgg
      541 gcgagaccgc gcgcgcccgc gcgcgacagc gcctcgaggt cgaacacgct ctcctgccgc
      601 acgattgccg cgagcagcag cgggtccacc ccgaactcgc gcgcctcggc ttccaccgcg
      661 gcccgccagg gccaggggta gatcgcccgc agcacccggg cgtcgctcac gccccgggcc
      721 gacgcgaccc agccaagtcg cactcccgcg gagccccacc cccgcatcga gagaccgtcg
      781 ctccacgcga gcaggtcgct gaccggttgc ggcgctcgtg ccagcagctc acgcacttcc
      841 agccgtgctt ccgtgtccag cccggcgagt aacagggtgt cgaggagggc caggcccgcc
      901 gtcgcggtcg gtgtggccgc ggtgctgtcg gcggcgaggg tcagcgactc catccccgcg
      961 gcgcgccgtg cccgcaggcc gtagtagccg atcgaatcct cccgcgccag cgcgcgccac
     1021 agcgaatccg cgcgggtggt gtccccgcgc tcacgggcca gccgcccgag ccagtaccgc
     1081 gccgcgttgc gctgtgcccc gcccaccaat acttccgcgg catagtagta cgcggcgctg
     1141 tccgggtccc cccggcgtcg cgccgcggcg gccatccgga atcgcgcgag cgaagaccgc
     1201 gcatcggcgg gatagcggcg ggtgagctcc gagaaccagc gctcggcccc ggcttcgtct
     1261 ccgcggtcgg tcaggaaatc gccgagcaga tagagggcgg tcggcgccgc ggtgtcgccg
     1321 gggaatgtct cggcgaaccc ggcgagcgcc tggggcgtgc cggggtcggc gatccgcacg
     1381 aacacccgac cacgacgata cagcgcgagc gccgtccagg cggtgtcgag ggccgccgcg
     1441 cggtaggcgc cggcggcatc cgggtagcgg cccagggacg tcagcagctc gcccatcagg
     1501 atgagcgtcg caccgctcga atccccctcg gaaagcgcac gtgtcagctg ctgacgggcc
     1561 tcggtcgcgg ccccgtgacc gcgcaacacg cgggcgagcg ccacccgatc cgcgggcgaa
     1621 cgcggcggga gcgggccatg ggcgagtgca acgcccgccg ccgcgtcgtc ggattgcggt
     1681 gcccgcccga acagggcata gagctccccg gccgccgccg ccgtgtcgcc cagggccagc
     1741 ccgagccgga ttgcgggcag ggtcagcccc gcatcgcgga acgccgcacg cgcatcgctc
     1801 gaatcgccgg cggtgagcag agcgccggcg cgcgcggcgg cggctccacg tgcggcggcc
     1861 ggcggcagat cgaccagcaa gcgccgcgcg gccgtcgtat cgcgcgagac gcgcgcctca
     1921 cgcaggcgca accacggctc gatcgaccgc agcccgccgc tgcgcgccgc ggcgtagtac
     1981 cgcgcggcgc tgtcgggaag cccggccgcc tcgaaggcca ttgcggcgcg cacagcgagc
     2041 agatcggcct ccggtctgcg ggcgcgtccg cgcgccgcga cgaagcgctg cgccgcgcgc
     2101 cccggctccc ccaggcgcca ctcggcttct cccagtaccc gcaacgcttc gccggctccg
     2161 tagtcctcga gccacggctg cccgaccagc aggctgcggg cccggtcgaa acgccgcgcc
     2221 tccagctccg cctcggcgct tcgtacgatg aacgcggcgt cctgtcgcgg gttgcgtgca
     2281 gccgcatcga gcagcgtctc ggccgtgtgc cagggacggc cggcgaccca gagtgactcg
     2341 gcgcgctgcg ccgggcgctc gctcgtctgc gctccggctg ccgcgatgcc cgccggccag
     2401 atcgcactca acaggcccag gactgcccgt cgcatcactg ctgccggcgc aagcgggcga
     2461 gcacctgcag gtagtcggcg cgggagcccg gggtgagctc gaagcggccg aacccggtca
     2521 ggtcctggaa gaggagaacc agacggagag tggtgtagct ccaccggatg ctctgcgggc
     2581 cgctgcgggt cgcgccgggg ctcgacagat ccatcacctc gtcgggttct ccgagggtga
     2641 tgaacacctc gccgcggtcg gtgagccagc cgggccgacc cgcttcctgg aagcggaaat
     2701 tcgcgatctg tatctgatgc aggtactcgt ccagcgcttc gttttccgga gtgagcgggg
     2761 ccggatcggt ggcggtccag aacgcgcgcc acgccgctgc ccgctgttcg ggtggcgcgt
     2821 tgcgcagctt ggcaacgagg tccggtcggt cgaagtaccg cagcagattc atcatctcgg
     2881 cgtagttcgt gagcacccac tggctggaga agctcaccag gaacggcgtc cggctctgct
     2941 gcgcgccgcc caccgcgccg aggacgaatt cctgctcgcc gaccggcagc tcgcttcccg
     3001 ccacgcgcac cacggcacgg gagaacccgg ccgagtcctg gaggggcagc gtgtcctgcc
     3061 acagcacgtt gtcgttccgg tccaccgctc gtgcggcaag ccgcgtgccg gagggcaatc
     3121 cgtagccctc gacgtagaag cgcagggtat ccgccccgta ggggagcgtg gcgcgcggat
     3181 tcagcagcag ctggggcggc tcccgcgtgg agctgcgacc gcttccctcg tagaacggca
     3241 gcggcccggc cagcgtggga ccgaccagtc gcggtacggt gtcggggcgc tcacgacgcg
     3301 cgtatgccgg actgttgcgg tcacgaatcg tcacgctcgc gttgtagaca ccggggcgca
     3361 gggaaagcat gccctgaaac agcacgcttt cgtcggcccg cagcgtttcc tgaaagccgc
     3421 ggacccgcac ctgctggtcg gaagcgagca tgaccggggc gccggaatcg gagcggaacg
     3481 ccagctcgac gtgatactcg gcgatgaact cgttgccctc ccggcggaag ctcagggagt
     3541 gattggcgag cgacagcgcg aacagcacca gggtggagtc gggcgaaggc cccgcgagaa
     3601 atcggatggt cgccacggtc ggcagcgggg cgccggtgac gaacaacccc atgcggtcgt
     3661 aaatcgtgcc cgcgtcgaac agtcgcgcga cctcgacgcc ggcctgcggg gtgttgggcg
     3721 aaccgacgcg ctgccaggtt ccgcaggcgg tgcatgaagc gatcgtcatt agggcggcag
     3781 ccgagcgtcg tgtcacttgc tgaacctcaa agccggacgt tagtttcgcc cgatcccatg
     3841 accagtgaga atctcgccgg tcgcaccatc gccggctatc gcctcctgga acgcgtcgga
     3901 gagggaggca cggcggaagt ctaccgcgcc gagcacgccg agcatggggc ctgtgcgttc
     3961 aaggtattgc ggccccgctt gcggggcgac cccaccgccg tcaagcggtt cctccgcgag
     4021 gcgagctacg gcgcgcgcgt gctccacccg tcggtcgttc gcacctacga ctacggggaa
     4081 gccgatgggc tgtattacct cgcgctccag tgggcgggcg ggcagtcgct ggcggacttt
     4141 ctacaccaga gcggccccgt cgctcctgcc gtggcggctg gcatcgtatc gtcgctggcc
     4201 gccggcctgg ccgcggcaca cgaagtcggc atcgtgcacc gcgacctgaa acccgaaaac
     4261 atcatccacg attccgccac cggcgtgacg aagctgctgg acttcggcat cgcgcgcgac
     4321 gcggagctgc ccccggaaga acgcctgacc cgcaccgggt tctttgtcgg tacgctgcag
     4381 tacgtggcgc ccgaggcgtt gtcgggggag ttggtggatg cgcgggcgga catctacagc
     4441 ctggcgacca tcgcctacta catgctctcg ggtcggcacc ccttcagcgg acgcaacccg
     4501 cgcgagctgt tccagcaact gttgcgagaa gcccccctgc cgctcaacga ggcgcttccg
     4561 ggccggcgct tccccgcggc cctggaggcg gccgtcatgc gggggctgag tcgcgatccc
     4621 gcacagcgcc aacccagcgt gacgagcttc gcggacgagt tcgccgccgc tgtggcgggg
     4681 acgaccgagg caccggggtc cagcctgctg ggcacgctgc gtcgcttcat gggcggcaag
     4741 aaggactagt agtagtgagt gccactacgc ctccgaatca cgaagacgcg attggccgac
     4801 tcgccgaact gctgcgacaa cgatccctga cccatgggga tttcgtcctc gcgtcgggcc
     4861 gccgctcgac ctacgacatc gatgcccgca agacgaccat gtcaggcgag gggcaggtgc
     4921 tcatcggcac cgcaggcctg ctcgctctcc agtcgcgagg ctggcacccc aggtccgtcg
     4981 gcgggctcac cctgggcgcg gatcccgtgg cctacgccat cgcccactcc gcccggctcg
     5041 ccggcacgtc gctcgacgcc ttcacggtac gcaagcaggc gaaggaccac ggcaccggcc
     5101 gccgcatcga ggggtgtctc gctgctgggg atgcggtggt cgtcatcgag gacgtcatca
     5161 ctaccggccg gtccgccctc gacgcggtgg cggccgctcg cgcggaaggc gccaatgtgc
     5221 tcggcgtgct cgccgtcatc gatcgccagg aaggcggccg cgaagcgatc gaaggcgccg
     5281 gactcgcggt cgtggtactg gtgaccgccc gggacctcgg ggtggaatga cgacgccggc
     5341 tgtgtccgac gccccgctcg tcgttctcca gctgagcgac tcgttcaacg agtactggcc
     5401 gctgctggcc cgggagcttg gcgtccccct ggtcgtccgc gacccggcca ctgacgtcga
     5461 ggccctgccg actcatgcgc tggcgatggt ggtcgccgcc ggtggggcgg aaacggaggt
     5521 cggtcccctc ctcggacgca tcgaccttcc cgcgtccatc cccctggtgg tcgctggagc
     5581 cagtacgtcg catcggatcg ccgcccacgt ggtcgccgtc ggggcggcgg actacttcgc
     5641 gctgcccgag gaccgcgacg cgctgcgcag caccctggcg gcggcactcg aacgcagtcg
     5701 cgcggcgcat gaccgtatcg cactggccac gctggaagcg caggcccacg ccttccgcga
     5761 aatcgtcggc gatagcccgg ccctccaggc ggcgttgtcg cgcgccgctc gcgtattgcc
     5821 acacgccgac gccacggtgc tggtgacggg cgagaccggt acgggcaagg agctgctggc
     5881 gcgcgcgctt cactacggag gcccgcgcgc ggcggcgccg ttcgtcgagc tcaactgcgc
     5941 cgcgctgccc agtgccttgc tcgagagcga gctgttcgga catgaacgag gggcgttcac
     6001 cgacgcgaag gccgccaaac ctgggctgtt cgaggtcgcg gcgggcggga cgttgttcct
     6061 cgacgaaatc gacagcctcg ccctggagct gcagagcaag ctgctgcgcg ccctggaaca
     6121 gaaagtcgtc cgtcgcctcg gggcgacgac cacgaggtcg ctggaagtcc gcatcgtcgc
     6181 cgccacgaac agccgcctcg accgcgcggt cgccgagggt cgtttccgcg aggatctgta
     6241 ctatcggctg aacgtcgtga cgctcgagtt gcctcccctg cgctcgcgcg gggacgacgt
     6301 cctccgcctg gtcgcacatt tcgtcgccaa gttctccaag cagtacacca tccccgagcc
     6361 ggccttgacc gccgatacgc gtcgcgcact gttggcgcac acctggcccg gcaatgtgcg
     6421 cgagctccgc aacatcgtc
//
LOCUS       DBNGZW010000744         5193 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1076398, whole genome shotgun sequence.
ACCESSION   DBNGZW010000744 DBNGZW010000000
VERSION     DBNGZW010000744.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1076398"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(38..1150)
                     /locus_tag="AC16JC_17420"
     CDS             complement(38..1150)
                     /locus_tag="AC16JC_17420"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5166536.1"
                     /translation="MGDSSAVAGRRWSRWVWWIAFWTLLGLVFATQLYLAERRLSARP
                     YTWWESVRADLPDWYIWGLFALLVARLTQRVPIDRVSWGFHFAFHVAASLLVALAHLS
                     VAVPLQWALRALAGEPYPILSKFVDNFAVFYLWNVLVYWAILAVVHSSEYRRSIRQRD
                     LRASQLESSVAEARLQALSMQLRPHFLFNTLNAIAELIHEDPDAAERMVGRLADLLRR
                     TLETDGSAEVPLATELELVDRYLDIQAVRFQDRLQVRWEVANDARTARVPAMILLPLV
                     ENAVRHGVSARPGPGEVGIRARREADTLRLEVWDDGPGMPLAAQNAGRRGIGLANTQA
                     RLVQLYGATHRFELVDGAPTGLVVRLSLPFRPERVA"
     gene            complement(1238..2743)
                     /locus_tag="AC16JC_17425"
     CDS             complement(1238..2743)
                     /locus_tag="AC16JC_17425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR5166537.1"
                     /translation="MSLWATKSIALLRAEADAQGEGTLRKTLGALNLTTLGIGAIIGA
                     GIFVLTGLAAARHAGPAVPLSFVAAGIACAFAGLCYAEMAAAVPVAGSAYTYAYATMG
                     EFMAWIIGWDLVLEYAMGASTVGVGWSGYFVSLLENFNIHLPSSLTSAPYRWCEASEV
                     AQGVAGCVTKGLNTTGAIANLPAVAIVAIMSTVLVIGIKESARVNNFIVALKLAVVGL
                     FIVAGLFFINTANWHPFIPPNQGTFGKFGLSGVLRGAGLIFFAYIGFDAVSTAAQEAK
                     NPQKDMPKGILGSLAICTVLYILVSGILTGIMPYTQLDVSHPVGFAVDNTPGLQWMGW
                     IINLGAVLGLASVILVMLLGQSRVFYSMSRDGLLPPWVGKIHPRFRTPYLTQIFVGLF
                     AAFFTGVFPIQLLGELVNIGTLLAFVLVCGGIIILRRTRPDLDRPFKTPWVPYVPILG
                     VLSCLGLMATLPGDTWIRLFVWMAIGLLIYFLYSRRHSTLQSGMDAKKIVS"
     gene            complement(2826..4283)
                     /gene="guaB"
                     /locus_tag="AC16JC_17430"
     CDS             complement(2826..4283)
                     /gene="guaB"
                     /locus_tag="AC16JC_17430"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HIR5166538.1"
                     /translation="MGTFRETPALTFDDVLLVPRHATVHPRSVDIASRFTRDIPLNIP
                     LVSAAMDTVTEAEMAMAIARAGGIGVIHKNMPIDRQAAEVDRVKRSESGMILNPITLG
                     PDRPLREAAQLMARFRISGVPIVDSAGKLVGIITNRDLQFERQLERPIREAMTKDDLV
                     TAPVGTTLDEAERILGAHRIEKLPVVDQQGVLRGLITIKDIFKRRQHPIANKDQHGRL
                     RVAAAVGGTPEAVTRAKALLDAGCDVIVVDSAHGHSQGVLDTVACLRETFPEAQLVGG
                     NVASEAGARALVERGVDAVKVGVGPGSICTTRVVTGVGVPQITAIVDAVRGAGDIPVI
                     ADGGIKYSGDIVKALAAGAESVMMGSMLAGTEESPGESFLLEGRRFKVIRGMGSLSAM
                     EEGSADRYFQEGEVSPSKLVPEGIEGRVPYRGPVGDVLFQMVGGLRSGMGYCGVSTVA
                     TLRTETEFIRITSAGLRESHPHDVVVTREAPNYSL"
     gene            complement(4328..4801)
                     /locus_tag="AC16JC_17435"
     CDS             complement(4328..4801)
                     /locus_tag="AC16JC_17435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166539.1"
                     /translation="MGCSRGGDRGGDAAAGRSDSGTPGQPAAAPVRGCAYITETEASV
                     VLAQPSRYRRAQSSAQLCTIEPVSGDAFHGTTVSYRVARGNTAQYDFFAAQKSAQPVS
                     GLGDRALWLSAGGTRGNLVVVMDTNVVSLAISDFSGRKDLRKRAEAFAKKVLNRL"
     gene            complement(4825..>5193)
                     /locus_tag="AC16JC_17440"
     CDS             complement(4825..>5193)
                     /locus_tag="AC16JC_17440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166540.1"
                     /translation="RERMLRKLETLSEEKVYQVLDYVEFLESKYAERPAGAAPFQKVA
                     ETLEDTMRAGRVPVTIIKGTMDAVGKAGKFFEGLAAAGKAAVDEVRKKSGDGTPPPAP
                     PAPPAPPPPPPAPETPPSES"
BASE COUNT          904 a         1798 c         1701 g          790 t
ORIGIN      
        1 gcgagcggct cgtcctcaac cagcagcgca cggatcgtca cgcaacgcgc tccggcctga
       61 acggcaacga caggcgcacc acgagtcccg tgggggcgcc gtccaccagc tcgaagcggt
      121 gcgtcgctcc atagagctgc accagccggg cctgggtatt cgcgaggccg atgccgcgcc
      181 gcccggcgtt ctgtgccgcc agcggcatgc cgggcccgtc gtcccacact tcgagccgca
      241 gggtgtcggc ctcgcgccgc gcgcggatcc ccacctcccc cggcccgggg cgggcggata
      301 caccgtggcg caccgcgttt tccacgagcg gcagcaggat catggcgggc acccgcgcgg
      361 tccgggcatc gttggctacc tcccagcgga cctgcagccg gtcctggaac cgcaccgcct
      421 ggatgtcgag gtagcgatcg accagctcca gctcggtggc gagcggcacc tcggccgagc
      481 cgtcggtctc gagcgtgcga cgcagcaggt cggcgaggcg tcccaccatg cgctcggccg
      541 catccggatc ctcgtggatc agctcggcga tcgcgttgag ggtgttgaag aggaagtgcg
      601 gccggagctg catgctgagc gcctggagcc gcgcctcggc cacgctcgat tcgagctgac
      661 tggctctgag gtcgcgctgc cggatagacc ggcggtactc actgctgtgc accacggcaa
      721 ggatggccca atacaccaac acgttccaga gatagaagac cgcgaagttg tccacgaact
      781 tggacaggat cgggtacggc tcgcctgcca acgcgcgcag ggcccattgc agcggtacgg
      841 cgacggagag atgagcgagt gccaccaaga ggctggcggc tacatggaaa gcgaagtgaa
      901 atccccagct gacccgatcg atcgggaccc gttgcgtcaa acgggcaacg agcagtgcga
      961 acagtcccca gatataccag tccggcagat ctgcgcggac ggactcccac caggtgtagg
     1021 ggcgcgccga gaggcgacgc tcggcgaggt acagctgcgt ggcgaacacg aggcccagta
     1081 gtgtccagaa cgcgatccac cagacccagc gcgaccaccg ccgtccagct acggcactcg
     1141 aatcacccac gctccgtccc tctgggccga cgtcactaat aatgccacgg ccggcctcat
     1201 ttccgaaggc cggccgtagc actcactcca ggagcggtca ggagacgatc ttcttggcgt
     1261 ccatccccga ctgcagcgtg ctgtggcgac ggctgtagag gaagtagatg agcaggccga
     1321 ttgccatcca gacgaacagc cggatccacg tgtcccctgg caacgtggcc atcagtccca
     1381 ggcaggacag cacgccgagg atcggcacgt acggcaccca aggggtcttg aacggccggt
     1441 ccaagtcggg tcgggtacga cgaaggatga tgatgccgcc gcaaaccagc acaaaggcga
     1501 gaagggttcc gatgttgacc agttccccca gaagctggat tgggaagacg ccggtgaaga
     1561 acgcagcgaa gagaccgacg aagatctgcg tgagataggg cgtccggaac cgtggatgga
     1621 tcttccccac ccacggcggc agcaacccgt cgcgcgacat ggaatagaac acgcgtgact
     1681 gcccgagcaa catcaccagg atcacggatg ccagccccag caccgcgccc agattgatga
     1741 tccatcccat ccactgcaga cccggcgtgt tgtccaccgc aaacccgacg ggatgcgaga
     1801 cgtcaagctg ggtgtacggc atgatgcccg tcaggatccc agataccagg atgtagagca
     1861 ccgtgcagat cgccaaggac cccagaatgc ctttcggcat gtccttctga ggattcttcg
     1921 cctcctgcgc ggccgtgctg acggcatcga atccgatgta cgcgaagaag atcaaccccg
     1981 cgccccgcag cacgcccgaa agcccgaatt tcccaaacgt gccctggttg gggggaatga
     2041 acgggtgcca gttggctgta ttgatgaaga acaatccggc gacgatgaac agtccgacca
     2101 cggcgagctt cagcgcgacg atgaaattgt tcacccgcgc tgattccttg atgccgatga
     2161 cgaggaccgt cgacatgatc gcgacaatcg ccacggcagg caggttcgcg atcgcgccgg
     2221 tggtattcag gcctttggtc acacaccccg cgacgccttg cgccacctcg gaggcttcgc
     2281 accatcgata gggagccgaa gtcaacgagc tcgggagatg gatgttgaag ttctccagca
     2341 agcttacgaa gtacccggac caccctacac cgaccgtcga tgctcccatc gcgtactcga
     2401 gcacgaggtc ccaaccgatg atccaggcca tgaactcgcc catcgtggcg tacgcgtacg
     2461 tataagcact gcccgccacc gggactgccg ctgccatttc cgcatagcag aggccggcga
     2521 acgcgcaggc gatgccggca gccacaaacg agagcgggac ggcagggccg gcgtggcggg
     2581 ccgcggcgag accggtgagc acaaaaatcc cggcaccgat gatcgcacca atgccgagag
     2641 tggtgaggtt gagcgcgccc agcgtcttcc ggagcgtacc ctcaccttgg gcgtcggcct
     2701 ccgctcgcag cagcgcgatc gacttggtag cccataagct catgcgaacc tcgtgcctgg
     2761 gaagtgtcag cgacccacga cgacggaacg cgctagacta gggctgatcc gacgaggtgt
     2821 caagctcaga ggctgtagtt gggcgcttcc cgcgtgacga cgacgtcgtg cggatgcgat
     2881 tcgcgcagcc cggccgaagt gatgcgaatg aactcggtct cggtgcgcag cgtggcgacg
     2941 gtcgagaccc cgcagtaccc catcccgctg cgcagcccgc cgaccatctg gaacagcacg
     3001 tcgccgaccg gtccccggta gggtacgcgt ccctcgatcc cttccgggac gagcttcgag
     3061 gggctcacct cgccctcctg gaagtagcgg tcggccgagc cctcctccat ggcagagagg
     3121 ctgcccatgc cgcggatcac cttgaagcgc cgcccctcga ggaggaacga ctcgccggga
     3181 ctctcttcgg tgcccgcgag catcgagccc atcatcacgc tctccgcgcc ggcggcgagc
     3241 gctttcacga tgtcgcccga gtacttgatg ccgccgtcgg cgatcaccgg gatgtccccg
     3301 gccccgcgta ccgcgtccac gattgccgtg atctgcggga cgccgacgcc ggtgacgacg
     3361 cgcgtggtgc agatcgagcc cggaccgacg cccaccttca ccgcgtccac cccccgctcc
     3421 accagcgcgc gcgcacccgc ttcgctcgcc acgttgccgc ccacgagctg ggcctcgggg
     3481 aacgtttccc gcaggcaggc gaccgtgtcg agcacgccct gcgaatgccc gtgggccgaa
     3541 tccaccacga tcacgtcgca gcccgcatcg agcagcgcct tggcccgggt cacggcctcg
     3601 ggcgtcccgc cgaccgcggc ggcgacgcgc aggcgcccgt gctggtcctt gttggcgatg
     3661 gggtgctgcc ggcgcttgaa gatgtccttg atggtgatca gcccgcgcag cacgccctgc
     3721 tggtccacca cgggcagctt ctcgatgcgg tgcgcgccga ggatccgctc cgcttcgtcc
     3781 agcgtggtgc cgacgggcgc ggtgaccaga tcgtccttgg tcatcgcctc gcggatgggc
     3841 cgctcgagct ggcgctcgaa ctgcagatcg cgattggtga tgatgccgac cagcttcccg
     3901 gccgagtcca cgatcggcac cccggagatg cggaagcgcg ccatcagctg ggccgcctcg
     3961 cgcagcggac ggtcgggccc cagcgtgatc gggttcagga tcatccccga ctcgctccgt
     4021 ttcacgcgat ccacttcggc tgcctggcgg tcgatcggca tgttcttgtg gatcaccccg
     4081 atgccgccgg cccgggcgat ggccatcgcc atctccgcct cggtgacggt atccatcgcc
     4141 gccgacacga gcgggatgtt gagcgggatg tcgcgggtga agcggctcgc gatgtcgacg
     4201 ctccgcggat gcacggtggc gtgccgcgga acgagcagga catcgtcgaa ggtgagggcg
     4261 ggggtctcgc gaaaggttcc catagcggaa gttactgcca cctcccccgt ttcctcaacg
     4321 ggggcgctca gagtcggttc agcaccttct tcgcgaatgc ctccgcccgc ttccggagat
     4381 ccttccggcc gctgaaatcg ctgatcgcca gggagacgac gttcgtatcc atcaccacga
     4441 ccaggttccc ccgtgtccct cccgccgaga gccacagggc gcggtccccg agtccggaga
     4501 ccggctgggc gctcttttgc gccgcgaaaa agtcgtattg cgccgtgtta ccgcgggcga
     4561 cccgatagct gaccgtcgtc ccgtggaacg cgtcgcccga caccggctcg atggtgcaca
     4621 gctgcgcact ggactgcgcg cggcggtagc gcgacggctg ggcgagcacg accgacgcct
     4681 cggtctcggt gatgtacgca cagccgcgga ccggcgcggc cgccggctgt ccgggagtgc
     4741 cgctgtcgga ccggcccgcg gcggcgtccc ccccgcggtc cccgccgcgg gagcagccca
     4801 cgacgaccag ggcgagtacg gcggtcagga ctctgatggt ggcgtctccg gtgccggcgg
     4861 gggcggcggg ggggcggggg gggcgggggg ggcgggggga ggcgtgccgt cccccgattt
     4921 cttgcgcacc tcgtccaccg cggccttccc cgcggcggcc aggccctcga agaacttccc
     4981 cgccttgccc accgcgtcca tcgttccctt gatgatggtc acgggcacgc ggcccgcccg
     5041 catcgtgtcc tcgagcgtct cggccacctt ctgaaacggc gccgcgcccg cgggccgctc
     5101 ggcgtacttg gactcgagga actcgacgta gtcgagcacc tggtagacct tctcctcgga
     5161 cagcgtctcc agcttgcgca gcatccgctc gcg
//
LOCUS       DBNGZW010000745         2605 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1826673, whole genome shotgun sequence.
ACCESSION   DBNGZW010000745 DBNGZW010000000
VERSION     DBNGZW010000745.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1826673"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /gene="ygfZ"
                     /locus_tag="AC16JC_17445"
     CDS             complement(<1..912)
                     /gene="ygfZ"
                     /locus_tag="AC16JC_17445"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005542 - folic acid binding [Evidence
                     IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAF17-like 4Fe-4S cluster assembly/insertion
                     protein YgfZ"
                     /protein_id="HIR5166541.1"
                     /translation="MTTPPSPPPLPTTVLRLPSGPWAAPVVQALRVGAVVAPADAGVI
                     ELAGGGAVACMQGLLTNDIEKPGDGSFVYGALLTPKGMIVVDGWVARLGTTVRFTVPL
                     SGGGRERALGILQRSVPPRLALSSDRTADVAVLRVAGTHALAIAEAARVPAPPLAGRV
                     ATVTLDGTPCEIARAAEGAPFALQISVAPADAAQVAVRLTQAGAFAALPPALELARVL
                     AGWPALAAEVDERTLPQEVRLDTIGGVSYTKGCYTGQETVSRLHFRGHTNRGLVGLEF
                     DGEPGPADPLPVFWEDREIGRVSTVAWL"
     gene            complement(909..1799)
                     /locus_tag="AC16JC_17450"
     CDS             complement(909..1799)
                     /locus_tag="AC16JC_17450"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR5166542.1"
                     /translation="MMTVTVRTGDGVDLALYRLDAVTPPSLGDRPAAKAVLLVHGAFS
                     SHTIWLRGGSRNAGLAVFLSAAGYDVWLADCRSHGAAAREPRPHTWHFEDTITQDAHA
                     LATYVRDVTGGIPPIWVGHSVGGAIGLALLAREPEALGAVVTLGTPGPVMGPLRRLFA
                     LGMIAICHTLGRFPARAFRMGPEDEAALILAEWMEWNTRGRWVGAGGFDYLAPLRGIR
                     TPVLTVAGEGDRMFAPPAACRALAEGVGVVPATFVVVGPKLDHPGLLLDPRADAECWP
                     LVARWIAALEPYRRSTSRSA"
     gene            complement(1796..2512)
                     /locus_tag="AC16JC_17455"
     CDS             complement(1796..2512)
                     /locus_tag="AC16JC_17455"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR5166543.1"
                     /translation="MLFRGCIMEGLFSHVHDATIRTLGVNGYAVSEVAGQGCCGALHA
                     HAGLHRTAQAQARRNIAAFGPGEAPIVVNSAGCGAQLKGYGALLDGEPAAVQFAARVR
                     DVTEILAERGPAPGAPLHLRVAYDAPCHLLHVQKVAEPPLAVLRAIPALDVRLTADSA
                     QCCGSAGLYGLLQPELSRAVLTAKLSSLLEDPPDVLATGNPGCVMQLGAGLLAARSRA
                     GARHPVELLDVSYRAAGFYR"
BASE COUNT          396 a          963 c          942 g          304 t
ORIGIN      
        1 cagccacgcc accgtggaca cgcgcccgat ctcccggtcc tcccagaaca cgggcaacgg
       61 atcggccggg ccgggctccc cgtcgaactc gagaccgacc agccctcggt tcgtgtgacc
      121 gcggaaatgc agccggctca ccgtctcctg tcccgtataa cagcccttcg tgtaggacac
      181 acccccgatg gtgtcgagcc gcacctcctg cggcagggtg cgctcatcca cctcggcggc
      241 gagcgccggc cagccggcga ggacccgcgc caactcgagc gccgggggca gggccgcgaa
      301 cgcgcccgcc tgagtgagcc gcaccgcgac ctgtgccgca tcggccgggg cgacgctgat
      361 ctgcagggcg aagggcgcgc cctcagcggc gcgggcgatc tcgcacggag tgccgtcgag
      421 cgtgacggtg gcgacgcgtc ccgccagggg cggtgcgggg acgcgcgccg cttcggcgat
      481 ggcgagggcg tgcgtcccgg cgacgcgcag caccgcgacg tcagccgtgc ggtcgctgct
      541 gagcgccagg cgcgggggaa ccgagcgctg caggatcccc agcgcgcgct cacggccgcc
      601 gccggacagg gggaccgtga agcgtaccgt cgttcccaga cgcgccaccc agccgtccac
      661 cacgatcatc cccttgggcg tcaacagagc gccgtatacg aaggacccgt cgcccggctt
      721 ctcgatgtcg ttggtgagca gcccctgcat gcacgccacc gccccgccgc cggcgagctc
      781 gatgacgccc gcatcggccg gtgccacgac cgcccccacg cgcagcgcct gcacgaccgg
      841 cgccgcccac gggcccgatg gcagccgcag cacggtcgtg ggaaggggcg gcggcgaggg
      901 aggggtggtc acgcagacct cgaggtcgag cgccggtacg gctccagcgc cgcgatccac
      961 cgggcgacga gcggccagca ctcggcatcg gcccgcgggt ccaacaggag gccgggatga
     1021 tcgagcttcg gtcccacgac gacgaaggtc gcgggtacca cgcccacccc ctcggccagc
     1081 gcgcggcatg ccgcgggcgg cgcgaacatc cggtcgcctt ccccggccac cgtcagcacc
     1141 ggggtccgaa tgccccgcaa cggggcgaga tagtcgaagc ccccggcgcc gacccagcgt
     1201 ccccgtgtgt tccactccat ccattccgcg aggatgaggg cggcctcgtc ctccggtccc
     1261 atgcgaaacg cgcgagccgg gaagcggccc agcgtgtggc agatggcgat catgccgagg
     1321 gcgaacaggc gccgcagcgg tcccatgacc ggccccggcg tgccgagcgt gacgacggcc
     1381 ccgagcgcct cgggctcgcg ggccagcagc gccagtccga tcgccccacc gaccgagtgt
     1441 ccgacccaga tcgggggaat gccccccgtc acgtcccgga cgtaggtcgc cagcgcgtgc
     1501 gcgtcctgcg tgatggtgtc ctcgaaatgc caggtgtgtg ggcgcggctc ccgcgcggcg
     1561 gctccgtggg agcggcagtc cgccagccag acgtcgtagc ccgccgccga gagaaacacc
     1621 gcaagccccg cgttgcggga gccgcctcgc agccagatgg tgtgactcga gaacgcgccg
     1681 tggaccagca gcacggcctt ggccgcgggg cggtcgccga gggacggggg cgtcacggcg
     1741 tccaaccggt agagcgcgag atcgacgccg tccccggtcc gtaccgtcac ggtcatcatc
     1801 ggtagaatcc cgccgcgcga taggagacgt ccagcagctc gacgggatgc cgcgcgccgg
     1861 cgcgggagcg ggcggcgagc agcccggcgc cgagctgcat cacgcatccg ggattccccg
     1921 tggccaggac gtcgggcgga tcttcaagca ggctggacag cttcgctgtg agcacggcgc
     1981 gcgacagctc cggctgcagc aggccgtaca ggccggccga gccgcagcat tgcgccgagt
     2041 ccgcggtgag tcggacgtcg agcgcgggga tggcgcggag gacggcgagg ggcggctcgg
     2101 ccactttctg gacgtggagc aggtgacagg gggcgtcgta ggcgacgcgt aaatggagcg
     2161 gcgcccccgg cgccggtccg cgctcggcca ggatctccgt cacgtcccgc acgcgcgcgg
     2221 cgaactgcac ggccgcgggc tcgccgtcca gcagggcgcc gtagcctttc agctgggcgc
     2281 cgcagcctgc gctgttcacg acgatcggcg cctctcccgg accgaaggcg gcgatgttgc
     2341 ggcgtgcctg cgcctgcgcc gtgcggtgca acccggcgtg ggcgtgcagt gcgccgcagc
     2401 aaccctgacc cgcaacctcg ctcaccgcat agccgttcac gccgagcgta cgaatagtcg
     2461 cgtcatgcac atgggagaac agtccttcca tgatgcaacc acgaaacaac aagacggcgg
     2521 tcaggcggtc aggcggtcgg gtggttcggg ggtcaggccc acgaaggcgc gttacaccga
     2581 ccgcccgacc gcccgaccgc ctgtc
//
LOCUS       DBNGZW010000746         2168 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_738872, whole genome shotgun sequence.
ACCESSION   DBNGZW010000746 DBNGZW010000000
VERSION     DBNGZW010000746.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_738872"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..627
                     /locus_tag="AC16JC_17460"
     CDS             <1..627
                     /locus_tag="AC16JC_17460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018570875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR5166544.1"
                     /translation="FLGRRDAQVKISGFRIEIGEIENRLLRLPSVREGAVVVTERAGK
                     GKQLVAFYSGERLDPAVVRDRLAASLPTYMVPSTVHWRETLPLTGNGKIDKQVLTALA
                     GELEVAGESREAPSTATEHRLAAAWAAVLGLPEDQIGRRDHFFELGGTSLSALRLAIA
                     LDRAVSFKDLSGHPILADQAELIDARGAPSAAALVPSGQCATTGGGVK"
     gene            624..1601
                     /locus_tag="AC16JC_17465"
     CDS             624..1601
                     /locus_tag="AC16JC_17465"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005457516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HIR5166545.1"
                     /translation="MTGERGSATATLNLDLQPGKPPLLVAEATGDAARWVAEQRGALR
                     ARVRETGSLRVRGLALREVAEIEAVFRQLGNLTLETEAFAPRRRYAPDVYSSSRWPPN
                     QPMCMHHELSYRLEVPGLMLFACLIAPLGGGATPVADAPAVLEALPADLVERFERLGW
                     LLIRNYTEDIGATLADAFGTDDHHAVERYCRANAIDFEWREGGGLRTWQRRSAVVRHP
                     ITGRRCWFNQIAFLNEWTLAPEVREYLVEEYGEDGLPFNTRCGNGDPVGADVVRTINE
                     AYEAHTVREPWEAGDLLLVDNIRTAHAREPFEGPREIVVAMAEPVRLGP"
     gene            1621..>2168
                     /locus_tag="AC16JC_17470"
     CDS             1621..>2168
                     /locus_tag="AC16JC_17470"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014087567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase family
                     protein"
                     /protein_id="HIR5166546.1"
                     /translation="MQLARGEAHCWSFPLDVPPETSAALSAILSEDERSRSARFRFER
                     DRRRFIVAHGALRELLGRYLGTHPEEIRFVTNPFGKPALDPELSSRLEFNLSHSADLA
                     LIAIAGAEVGVDLEHIRAHPDYAAIAEDFFSAAEADSLNSLPSHLQAEAFFSCWTKQE
                     AYVKARGEGLATTTDPVSVPADL"
BASE COUNT          324 a          748 c          779 g          317 t
ORIGIN      
        1 ttcctcggcc ggcgcgacgc gcaggtcaag atctcgggct tccggatcga gatcggcgag
       61 atcgagaacc gactgctgcg cctgcccagc gtccgcgaag gcgcggtcgt ggtcaccgag
      121 cgggccggca agggcaagca gctggtggcc ttctactccg gcgaacggct ggacccggcc
      181 gtggtacggg accggctcgc cgcgtcgctg cccacgtaca tggtcccgtc cacggtgcac
      241 tggcgggaaa cgctgccgct cacgggcaac gggaagatcg acaagcaggt gctgacggcg
      301 ctcgccggag agctggaggt cgcaggggag agccgcgagg caccgagcac ggcgaccgag
      361 caccggctgg cggcggcgtg ggccgccgtg ctcggcctcc ccgaggacca gatcggcagg
      421 cgcgaccact tcttcgagct gggtgggacg tcgctctccg ccctgaggct ggcgatcgcg
      481 ctggaccgcg cggtgtcctt caaggacttg agcgggcatc cgatcctcgc cgatcaggcg
      541 gagctgatcg acgcccgggg cgccccttcg gctgcggcac tcgtgccgag cggccagtgc
      601 gcaacaacgg gagggggggt caaatgaccg gcgagagagg gtccgcgacc gcaacgctca
      661 acctggacct gcaacccggc aagccgccgc tgctcgtggc cgaggcgacc ggcgacgcgg
      721 cgcgctgggt ggccgagcag cgcggcgcgc tgcgggcccg ggtcagggag accgggtcgc
      781 tccgggtgcg tggtctggcg ctgcgtgagg tggccgagat cgaggcggtg tttcggcagc
      841 tgggcaacct gaccctcgag acggaagcct tcgcgccccg gcggcgctac gcgccggacg
      901 tgtactcctc gtccaggtgg ccgcccaacc agcccatgtg catgcaccac gagctcagtt
      961 accggctcga ggtccccggc ctcatgctgt tcgcttgcct gatcgcgccg ctgggcgggg
     1021 gggccacccc cgtggcggac gcgccggcgg tgctggaggc gctgcccgcc gacctggtcg
     1081 agcgcttcga gcggctgggc tggctcctga tccgcaacta caccgaggac atcggggcga
     1141 cgctcgccga cgcattcggc accgacgacc atcacgccgt cgagcgctac tgccgcgcca
     1201 acgcgatcga cttcgagtgg cgggagggcg gcgggttgcg cacctggcag cgccgcagcg
     1261 ccgtcgtgcg tcacccgatc accggccggc ggtgctggtt caatcagatc gcattcctga
     1321 acgagtggac gctggccccg gaggtgcgcg agtacctggt ggaggagtac ggtgaggacg
     1381 ggctgccgtt caacacccgc tgcggcaacg gcgatccggt cggtgcggat gtcgtccgga
     1441 cgatcaacga ggcctacgag gcccacaccg tccgcgagcc gtgggaggcg ggcgacctgc
     1501 tgctggtgga caacatccgc accgcgcacg ccagagagcc cttcgagggc ccccgcgaga
     1561 tcgtggtcgc gatggccgaa ccggtgcgcc tgggcccctg acgggggcgg gggggatccg
     1621 gtgcagctgg cgcgtggcga agcgcactgt tggtcgttcc cgctggacgt tccgcccgaa
     1681 acgtccgccg ccctctcggc gatcctcagc gaggacgaac ggagccggag cgcccggttt
     1741 cgctttgagc gcgaccggcg ccgctttatt gtcgcgcacg gtgccctgcg tgagctgctg
     1801 ggacgctacc tcgggaccca ccctgaagaa atccgcttcg tgaccaaccc gttcggcaag
     1861 ccggcgctcg accccgagct cagcagccgg ctcgagttca acctctcgca ttcagccgat
     1921 cttgccctca tcgcgatcgc cggcgccgag gtcggtgtcg atctcgaaca catccgggcc
     1981 caccctgatt acgccgcaat cgcggaggac ttcttctcag ccgccgaagc tgactccctg
     2041 aacagccttc cgagccatct ccaggccgag gccttcttta gttgttggac gaagcaggag
     2101 gcgtacgtca aagcccgtgg cgagggtctc gcgacgacga ccgatcccgt gtccgtgccg
     2161 gcagatct
//
LOCUS       DBNGZW010000747         2017 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1017343, whole genome shotgun sequence.
ACCESSION   DBNGZW010000747 DBNGZW010000000
VERSION     DBNGZW010000747.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1017343"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..947)
                     /locus_tag="AC16JC_17475"
     CDS             complement(<1..947)
                     /locus_tag="AC16JC_17475"
                     /inference="COORDINATES: protein motif:HMM:NF012544.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR5166547.1"
                     /translation="MSAPPPQRRELTRTLRQGDLVLVVIGTVIGSGIWLVPGTVLRNT
                     ADDPGVALLVWLAGGICSLLGALTFAELGALHPDAGGTYTYVREAFGRFPAFLLGWAL
                     FLAINTGSTATLAVAFATYAGELVSLGPVGRKVLPVLMILAVSAVNIRGVRHAATVQN
                     WSTAVKVAAILAIAVSGIALGSGGTPTMDRAFSTPLSPGLLSGAGVALLGVLWAYEGW
                     INVTNSAGEALDPQRTFARGIIVGTAALVALYLLANIGYLAALGPDGVAGSQRVAADT
                     VRELFGPVPAKFVSAAVLVSVFSAANGLALTGPRMYFAMA"
     gene            complement(944..>2017)
                     /locus_tag="AC16JC_17480"
     CDS             complement(944..>2017)
                     /locus_tag="AC16JC_17480"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR5166548.1"
                     /translation="TRAPRAGEWVRNPDLARSFREIAAQGPGALYGGDLGQRIVTRVR
                     ELGGYLTLDDLKNHHPEWVEPISAPFHGYRVWELPPNNQGLAVLEMLRLLEPYDLKSL
                     GHNSAAYLHLLIESKKLAFADVARFVGEPKALTVPPAALLTDAYVAARRALIDTARAA
                     DRPQPGKPLTASETIYLTVADSAGNMVSFINSLYDLFGSGVVVPGTGFALQDRGAGFT
                     LEPGLPNTVAPGKRPFHTLIPGFVTRPDSAGVEQPWLSFGVMGGSMQPQGHVQVLLNL
                     LVFGMDLQDAIDAPRFRHFDGLRVALEPAIGDDVRRRLAALGHVIVPDSGIAFGGAQF
                     ILRLPRGYAAGSDPRKDGMAAGH"
BASE COUNT          338 a          704 c          722 g          253 t
ORIGIN      
        1 gccatcgcga agtacatgcg cggcccggtg agcgcgaggc cgttggccgc gctgaacacc
       61 gacacgagca ccgcggcgct gacgaatttc gccgggacgg gtccgaacag ctcgcgcacc
      121 gtatcggccg cgacgcgctg cgagccggcg acgccgtccg gccccagagc ggcgaggtag
      181 ccgatattgg cgagcaggta gagggctacc agcgccgccg ttccgacgat gatgccgcgg
      241 gcgaaggtgc gctgcgggtc gagcgcttcg cccgccgagt ttgtcacgtt gatccaccct
      301 tcgtaggccc acagcacgcc gagcagggcg acgcccgcgc ccgagagcaa gccgggcgag
      361 agcggcgtcg agaacgcgcg gtccatggtc ggcgtgccgc ccgagccgag ggcgatcccg
      421 gacacggcga tcgcgagaat cgccgccacc ttcaccgcgg tgctccagtt ctgcaccgtc
      481 gccgcgtggc gcacaccgcg gatgttcacc gcggaaacgg cgagaatcat cagcacgggc
      541 agcaccttgc ggcccacggg cccgagcgac acgagctcac cggcataggt cgcgaacgct
      601 acggcgagcg tcgccgtgga cccggtgttg atcgcgagaa acagggccca accgagcagg
      661 aacgccggga accggccgaa cgcctcgcgc acgtatgtgt acgtgccacc cgcgtcggga
      721 tggagcgcgc ccagctccgc gaaggtcagt gcgcccagga gcgagcagat cccgcccgcc
      781 agccagacga gcagcgccac gccgggatcg tcggccgtat tgcggagcac ggtgccgggc
      841 acgagccaga tcccggaccc gatgaccgtg ccgatgacca ccaggacgag atcgccctgg
      901 cgcagcgtgc gggtgagctc gcgccgctgg ggcggtggcg cgctcaatgt cccgccgcca
      961 tgccgtcctt gcgcggatcg gacccggcgg catagccacg cggcagccgg aggatgaact
     1021 gggcaccgcc gaaggctatt cccgagtcgg ggacgatgac gtgaccgagg gcggcgagac
     1081 gccgccgcac gtcgtcgccg atcgccggct cgagcgccac gcgcaggccg tcgaaatgcc
     1141 ggaagcgcgg cgcgtcgatc gcgtcctgca ggtccatccc gaagaccagc aggttcagca
     1201 gcacctggac gtgtccctgt ggctgcatcg agccgcccat gacgccgaac gagagccacg
     1261 gctgctcgac gccggcggaa tcgggtcgag tgacgaagcc cgggatcagc gtatggaagg
     1321 gacgcttccc cggcgccacg gtattgggca gccccggctc gagcgtgaag ccggcgcccc
     1381 ggtcctggag cgcgaagccg gtgccgggca cgacgacgcc ggacccgaac aggtcgtaca
     1441 acgaattgat gaacgagacc atgttgcccg ccgagtcggc gacggtgagg tagatcgtct
     1501 cgctcgccgt aaggggcttc ccgggctgcg gccggtccgc cgcgcgggcg gtgtcgatga
     1561 gcgcgcggcg tgccgccacg tacgcatcgg tgagcaacgc ggcgggcggg acggtcagcg
     1621 ccttcggctc accgacgaaa cgcgccacgt cggcgaaggc gagcttcttc gactcgatca
     1681 gcagatgcag gtaggcggca ctgttgtgcc ccagcgactt gagatcgtac ggctcgagca
     1741 gccgcagcat ctcgagcacc gccaggcctt gattattcgg cggcagctcc cacacccggt
     1801 agccgtggaa cggcgccgag atcggctcca cccattcggg gtggtggttc ttgaggtcgt
     1861 cgagcgtgag gtagccgccc agctcgcgca cccgcgtcac gatccgctgt cccaggtcgc
     1921 cgccgtacag cgctccgggg ccctgcgccg cgatctcccg gaagctccga gccaggtcgg
     1981 gattgcggac ccactcaccg gcacgcgggg cgcgcgt
//
LOCUS       DBNGZW010000748        12096 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1079135, whole genome shotgun sequence.
ACCESSION   DBNGZW010000748 DBNGZW010000000
VERSION     DBNGZW010000748.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 12096)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12096)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12096
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1079135"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(51..710)
                     /locus_tag="AC16JC_17485"
     CDS             complement(51..710)
                     /locus_tag="AC16JC_17485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166549.1"
                     /translation="MNRALVVTLALAAAGASARAQTVLLGVHGALGDYREVSSNLRHG
                     GGGFGGSLALRVGRLSAEGIVTRVSYTPHDDTAGLPSFKSTQLDARFGVDVSGGLAAE
                     VGVLRRTISPELAAQEMGATRIGARYTKVIGPGATVGLRGDYLAGSKFTGEGSAGLAF
                     ELGLFASVGPSNGRFRGTADYSFQRVDRHVDRKVGSVKVGSAKVPIQQSLVRLGVAVG
                     F"
     gene            complement(707..1741)
                     /locus_tag="AC16JC_17490"
     CDS             complement(707..1741)
                     /locus_tag="AC16JC_17490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5166550.1"
                     /translation="MRQAVVVLVTLLLPAASVAQGVIQSPALRRAVAAYEAVDFAQVI
                     VQARAALRERLTAPERARAEELLGFAFSATNQPDSAIAAFREAIQLDPDRQLDPRRIS
                     PRITGYFNAALGQVLVVRQLKVDTARFVSGTAGAGIPIRFTITTPARVRTRAVSGANT
                     LLIDSGVVVGTYNLRWPATRAGGDPVPPGTWRILVEAFGSGQYTTSAYQDVQITAGAV
                     DTLPHLTALPGYSELPETEIPPQSGRPLGIALLYTGVAGAGALALNNGDLGTATGREI
                     GVAAGAALITGFVMMLRKPAPRPALGNIQYNRLLKEQLARRNADIALQNAQLRRQVQL
                     TAVPIPKVAR"
     gene            complement(1753..3462)
                     /locus_tag="AC16JC_17495"
     CDS             complement(1753..3462)
                     /locus_tag="AC16JC_17495"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5166551.1"
                     /translation="MTQCVKCHTPLPDNSRFCFACGADQTGGGVDARASGPVEALQAR
                     LQRLVQGKYEILRLLGKGGMGAVFLAHDLMLEREVAIKVLPPDFSTDPQVIKRFQQEA
                     KTAAKLDHNNIIPIYRVESEEGLNYFVMKYISGTSLEDVLESKQPLTYDYVQRILWEA
                     ACALGHAHSRGVVHRDVKPANIMFDHDGRVMLTDFGISKALQSASGLTGTGMIIGTPH
                     YMAPEQAKGQPVDGRADQYSLGVVAYRLLAGSLPFSGDSVHTILYKHIFEEAPRVSAL
                     RKDIPRFLTDPIQRALSKDPGERFPTMEDFATAIWPEQPVSATGKATPRPLRPRPAPG
                     ARTGAPVTSDAATEHVASAAAAPTTPLPSVRARTGPPSPARPAAQRSSKSGWFVLLAL
                     VAVGAGGYVWWSGRSGAGGGGEPSATNAPAAPPAANPAPAATPQSLVVATQLPGTTAT
                     AVTPPPSQPTPPAADRGATAPRREPQRQAPTRRSSPPVVQQEPPPAPVEAQGLLTIDS
                     DPAGEVFIDGVDVGPTPLVNYAVKVGAHQLRIEAPGFRTYAKPIQVAAGPVRLGRVTL
                     TPQ"
     gene            complement(3459..5213)
                     /locus_tag="AC16JC_17500"
     CDS             complement(3459..5213)
                     /locus_tag="AC16JC_17500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HIR5166552.1"
                     /translation="MGFPVRALGLGAAWAVVLASCSVPTDKAKDIQVVVRLSDSLQSR
                     GILGQGLRDSVYALAFRVGATGDTQPVANAGFQWSPSDPSIATVEGKPNGWAIVTGLK
                     PGFVQLTAAPMAYEQASSGSATVRVAGRFAIDSVRPNVVHYGEKVTVYGVGVNQLFAL
                     TLGADLFPDRFSLQGNADGLSSEAWWVPYPASTGRPLYFAPGGSDTVAVPVAVVDTDL
                     FEPDSAAPAPVVATGPGGPRVIANLPVLYFNPALYFEPVDFTVPRVNLPVDWLSFAIS
                     DSTQPISFQVTSTVFNDTSAAFTYFADSVEYNTCSGLYCVSPTSRIIFPGTQWICNGQ
                     FFFSNGVRLPQVVAAFTHSFSSHPEFIQFYSTPGRYELAVVRGYVSRLPPDRFEENDL
                     WCMDADSTFTQSRDSNPASPTAKHIVIGRAGAGQNGFWFDSLLTMDNPGDADFIRFRV
                     QPPVAPADTFVTIHVKSRPFGVLDPSDVDAYVVRVSDFAFMGGSASVGSDESMTLNLA
                     AGDYYLAVVDVAAQPTRYSICIVKGLGCVPPGAPPQLTPAPSVTRLPRRDPHRDPTVP
                     PTGLPLPLSNGRISSPHR"
     gene            complement(5280..6689)
                     /locus_tag="AC16JC_17505"
     CDS             complement(5280..6689)
                     /locus_tag="AC16JC_17505"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR5166553.1"
                     /translation="MSRGDLVDLLLFTVVAGGFVGLWYLVGAYAGVLWSRRALVLLAC
                     SAGIALLGFRFPGALDLLLLADAMLVAGVWLDALLAPRIAHREITVSREAPPAFSLGR
                     AGEVAYRWRNAGSRRGWLRVREIRPDVLGGTLEPREVRVPADGDMREVLPVVPVRRGH
                     EIAGGFAVDSGGPLGLGVRRSRLALPWDARVYPALTSVRRRAAVAQALRRREQGRQPL
                     RRLGEGRLFESLRDFVPGDDPRLIDWKATARRRKLITRQFEAERRQQVLLVLDAGRLL
                     TADVAGVARMDYVVQAALELAYAAAQHDDNVGVMVFADGVKHFVTPQRGRRGLKQVLD
                     VLAEVTPALVEPDYPGAFRYLAARNRKRALTVVFTDVIDRFASEALVANLGTLRPRHL
                     PLAVTLRNPELDGLAALRPEQARDAWRKAAAEELLRSREEALSHMRRAGVIVLDVPPA
                     RAAEAVVDKYLELKRRGRL"
     gene            complement(6686..7642)
                     /locus_tag="AC16JC_17510"
     CDS             complement(6686..7642)
                     /locus_tag="AC16JC_17510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003975794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR5166554.1"
                     /translation="MGGIASAGVARGILEALNRVVLGQEKATREVLVALLARGHVLLE
                     GVPGTAKTLLARTLALALDVRFQRIQFTPDLMPADITGVSMLTGSHEFTFRPGPIFAD
                     LVLADEINRAPAKTQAALLEAMQERTVTVDGVSHSLSPVFTVFATQNPIEFEGTYPLP
                     EAELDRFMVKAQFGYPAADVEQGILTRYVEGFEADRPATYGVSRVADARGLEEVRAAV
                     ESVRVEPQITAYITAVVRATREAASLTLGASPRAGVSLLKAARAAALLDEREYVVPDD
                     VKTLAPSVLRHRVSVAPELELEGVTPDAALKAILDKTEVPTA"
     gene            complement(7591..8877)
                     /locus_tag="AC16JC_17515"
     CDS             complement(7591..8877)
                     /locus_tag="AC16JC_17515"
                     /inference="COORDINATES: protein motif:HMM:NF025617.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HIR5166555.1"
                     /translation="MSNRTEIALAVLLAVALIVALMAGHHQRRAVSRWEPVSTYTTGP
                     TGGRGPYDVLARLGVPVDRRRTPLFDLARQARGRPAVLAVVSPEVWLESAEREAVARY
                     VDAGGMVVAVADAGGLTRCFGWDTAEPDSADLAADDSAAVVPPEGLATLPRVGWVLRP
                     FGQDSLEGPTKRTAELAKRGRLARPSHDDDDCRAAQRDGVDTLLATPHHAPVVLRLRF
                     RGGGSALLVADEGYFRNAAWRSTDVPDLLVPLMLPATRGRISWDEYHHGYGEQTSRGG
                     ALVGWMAHSPVGWVLFQLAAVVLLWLAVRAVRFGPARAVVERRRRSPLEHVEALAAGL
                     EGAGGVDTAVALTISGLRRRLSRTGQPPHGDARQWLASLELALPGARGRRAARRLQET
                     LTKPGGNERVLAAAQAVEDVWEELRPRASPAASWKR"
     gene            complement(8861..9472)
                     /locus_tag="AC16JC_17520"
     CDS             complement(8861..9472)
                     /locus_tag="AC16JC_17520"
                     /inference="COORDINATES: protein motif:HMM:NF024948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HIR5166556.1"
                     /translation="MTVPADSVRRAVREVFARPEYDWVQTARPLHWLRDLWMRFRAWL
                     DAFATQHPLGAKLLFWAALLALIALLAHIGLTVWRIYRVTVQQPGGTGAAPAPELLDS
                     RGHLARAESLARTGRYTEALAHRFIVLLLELDRADALTFHPSKTPAEYVREARLGATG
                     RATMAGLVSRLYHHVFGAEPLDERGYREFGAAAGTLLHHVEPH"
     gene            complement(9493..10392)
                     /locus_tag="AC16JC_17525"
     CDS             complement(9493..10392)
                     /locus_tag="AC16JC_17525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166557.1"
                     /translation="MADLPPIEQMEFGEILDGALSLFRRHFPLFLKLSLAVLWLPVVL
                     GLYWRVRFFGAEATTPEETVAVLQDQIVPLVLWTLVFAIFYLCAMLLLTAGSIRIISD
                     SYLGREPEFGAVWRFGFGKIVPLFLVGLGKGLLLMLVGMVCLFVGAIVVAVAKGLGTA
                     IMALAILGLFVGSIWAVLFIACGYMVTTPAVVLERLSSAFDSFGRSWELTRGAKLRML
                     GLVLVGWLISSLLPAIVVQAIGALVVALIPGAQLYWTIVGSVLPIMLAPILPCILTLA
                     YYDLRVRREGFDLQLLSEQLGAT"
     gene            10435..>12096
                     /locus_tag="AC16JC_17530"
     CDS             10435..>12096
                     /locus_tag="AC16JC_17530"
                     /inference="COORDINATES: protein motif:HMM:NF014052.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HIR5166558.1"
                     /translation="MSARTRAPLQPSGLRQHLGVETPEHILLSFELAGLGSRAAAAFA
                     DLVVLSVAYFIIFVFYEAAGLSESWDSGLGSWGTALVVLVASLGFFLYFALFEALWEG
                     RTPGKRALGLRVVLDTGRPVTANAATVRNLVRIVDFFFPLGPLPGLAFVFFHPANKRL
                     GDLAAGTIVVRDRVAAWRLAAAPPAPANEALELGPPELSDPEFALLDRFLARVDQLLP
                     AVRDRMAADLSRRIEAKIPRRASDPLAYLALVFADEQQKRRGRFATRATGAAGRITVT
                     AERFFARKRDTWEAFREAAQRYERGGLAQVPAAEIPAFAARYREVAADLARARTYGVA
                     PQVVAYLERLVGAGHSVLYQARATRRPPFFRTVLADFPAAVVQSWVYVLASFLLFMIP
                     AVIGYVMLRERPRLADEIVSPVMVSRAEQAAERAEEGIGYAQAESGERPVIAAAIISN
                     NVRVCIMAFAGGMLAGILTLWALVFNGLAIGTGFGVFANYHAAGYLATFVAGHGVLEL
                     TAIFISGGAGFRLAHALIAPGDRTRLDALVVDGRVAMRMIGAVVFL"
BASE COUNT         1882 a         4344 c         4221 g         1649 t
ORIGIN      
        1 tcggctatcg gctatcggct atcagccaag ccgacagccg acagtcatcc tcagaatccc
       61 accgccaccc ccagccgcac cagcgactgc tggatcggga ccttggcgct ccccaccttc
      121 acgctcccca ccttgcggtc gacgtggcgg tcgacgcgct gaaagctgta gtccgcggtg
      181 ccgcggaagc gtccattcga cggtcccacg gacgcgaaca agccgagctc gaaggccagc
      241 cccgccgagc cttcacccgt gaatttcgac ccggccagat agtcgccgcg cagtccgacc
      301 gtcgcccccg ggccgataac cttggtatag cgagcgccga tgcgcgtcgc gcccatttcc
      361 tgggcggcga gttcgggaga gatggtgcgg cgcaagacac cgacttcggc cgccagcccg
      421 ccggacacgt cgacgccgaa ccgcgcgtcg agctgcgtgg acttgaagct cggcagtccc
      481 gcggtgtcgt catgcggcgt gtagctcact cgcgtcacga ttccctcggc gctcaggcgg
      541 ccgacgcgca gcgccagtga cccaccgaac ccgccgccgc catggcgcag gttggacgat
      601 acctcccggt aatcgccgag cgcgccgtgc acgccgagca ggaccgtctg ggcacgggcc
      661 gaagcccccg cggcggccag cgccagcgtg acgacgagcg cgcggttcat cgggccacct
      721 tgggaatcgg caccgcggtg agctgcacct gacgacgcag ctgggcgttc tgcagcgcga
      781 tatcggcgtt gcgacgcgcg agctgctcct tcaacagacg gttgtactgg atgttgccga
      841 gcgccggccg gggcgccggc ttgcggagca tcatcacgaa gcccgtgatc aaggcggcgc
      901 cggccgcgac tccgatctcc cgtccggtgg cggtgccgag atcgccgttg ttcagcgcga
      961 gcgcacccgc gcccgcgacg cccgtgtaca gcagcgcgat gcccagcggc cggccgcttt
     1021 ggggcgggat ttcggtttcg ggaagctcgc tgtacccggg tagcgccgtg agatggggca
     1081 gcgtgtccac cgcaccggcc gtgatctgca catcctggta agcggaggtg gtgtattgcc
     1141 ccgacccgaa cgcctcgacg aggatgcgcc acgtcccggg cgggacggga tcgccgccgg
     1201 ccctcgtcgc cggccaccgg agattgtacg tccccaccac gacgccggag tcgatcagca
     1261 gagtgttcgc gccgctcacg gcgcgcgtgc gcacccgcgc gggggtcgtg atggtgaagc
     1321 gaatcgggat gcctgcgccc gccgtcccgg agacgaaccg ggccgtatcg accttcagct
     1381 gccgcaccac gagcacctga ccgagggcgg cgttgaagta gccggtgatg cgcggcgaga
     1441 tgcgccgggg atcgagttgg cggtcggggt cgagctgaat cgcttcccga aacgccgcga
     1501 tcgccgaatc aggctggttg gtcgcgctga aggcgaaccc gagcagctcc tcggcacgcg
     1561 cgcgctcggg cgcggtgagc cgctcccgca acgccgcccg agcctgcaca atcacctggg
     1621 caaagtcgac cgcctcgtag gcggccaccg cgcggcggag cgccggactc tgaatcaccc
     1681 cctgcgcgac tgaggcggcg ggcaggagca gggtgaccag aacgacgacg gcctgtcgca
     1741 tcagcctcta cctcactggg gagtcagggt cacgcgcccc agccgcaccg ggccggcggc
     1801 gacctggatc ggcttcgcgt acgtccggaa gccgggggct tcgattcgca gctggtgagc
     1861 ccccaccttc accgcgtagt tcaccagcgg ggtcggcccg acatccacgc cgtcgatgaa
     1921 tacctccccc gccggatcgg agtcgatcgt cagcaacccc tgcgcttcga ccggtgccgg
     1981 cggcggctcc tgctgcacca cgggcggcga cgaccgacgc gttggggcct gccgctgcgg
     2041 ctcgcgccgc ggagcggtcg cgccgcgatc ggccgccggc ggcgtcggct gactcggcgg
     2101 cggggtgacg gccgtcgccg tcgtgcccgg gagctgcgtc gccaccacga gcgattgcgg
     2161 tgtggccgcg ggcgccggat tcgccgccgg cggcgcggcg ggagcattcg tggccgacgg
     2221 ctcgccgcct cccccggcgc cgctccggcc gctccaccac acgtatccgc ccgcccccac
     2281 cgccaccagg gcgagcagca cgaaccagcc ggatttggac gaccgctgcg ccgcgggccg
     2341 ggccggggag ggggggccgg tgcgggcccg caccgacggc agcggcgtcg tgggcgcggc
     2401 ggccgcggac gcgacgtgct ccgtagccgc gtcggacgtc accggcgcac cggttcgcgc
     2461 accgggtgcc ggccgcggcc gcagcggacg cggcgtcgct ttccccgtgg ccgatacggg
     2521 ctgctccggc cagatcgcgg tggcgaagtc ttccatcgtg gggaagcgct cgccaggatc
     2581 cttggagagg gcgcgctgaa tcggatcggt caggaagcgg gggatgtcct tgcgcagcgc
     2641 ggagacgcgc ggggcctcct cgaagatgtg cttgtagagg atggtgtgca ccgagtcgcc
     2701 gctgaacggc agggagccgg ccagcaggcg gtacgcgacg acgccgagcg agtattggtc
     2761 cgcgcggccg tccaccggct gccccttggc ctgctcgggc gccatgtagt gcggcgtgcc
     2821 gatgatcatg ccggtaccgg tgagcccgct cgccgactgc agggccttgg agatgccgaa
     2881 gtcggtgagc atcacgcgcc cgtcgtggtc gaacatgatg ttggccggct tcacatcgcg
     2941 gtgcaccacg ccgcgcgaat gcgcgtggcc cagcgcgcag gcggcctccc acaggatgcg
     3001 ctgcacgtag tcgtaggtga gcggctgctt cgattccagc acgtcctcga gcgaggtgcc
     3061 cgagatgtac ttcatgacga agtagttgag cccctcctcg ctctccaccc ggtagatcgg
     3121 gatgatgttg ttgtggtcga gcttggcggc cgtcttcgct tcctgctgga accgcttgat
     3181 gacctgcggg tcggtcgaga agtccggggg caggaccttg atcgcgacct cgcgctcgag
     3241 catcaggtcg tgcgcgagga acacggcgcc catgccgccc ttgccgagca gcctgaggat
     3301 ctcgtacttg ccctgcacca gccgctgcaa ccgggcctgg agcgcctcca ccggtccgga
     3361 cgcccgcgcg tccaccccgc cacccgtctg gtcggctccg caggcgaagc agaaccggct
     3421 gttatcgggg agcggggtgt ggcacttgac gcactgcgtc accggtgcgg cgatgagatc
     3481 ctcccgttcg acaggggcag cggcagcccg gtgggcggca cggtcggatc gcgatgcgga
     3541 tcgcgccgcg gcaggcgggt caccgagggc gccggcgtca gctgcggcgg ggcgccgggc
     3601 ggcacacagc ccaacccttt gacgatacag atgctgtagc gcgtcggctg cgccgccacg
     3661 tccaccaccg cgagatagta gtcaccagcg gcgaggttga gggtcatcga ctcgtcggag
     3721 ccgaccgacg ccgagcctcc catgaaggcg aagtcactga cccggaccac gtacgcgtcc
     3781 acgtccgacg gatcgagtac cccgaacggc cgggatttga cgtggatcgt gacgaaggtg
     3841 tccgcgggcg ccacgggcgg ctgcacgcgg aaccggatga agtcggcgtc tcccgggttg
     3901 tccatggtca gcaacgagtc gaaccagaac ccgttctgac cggcgcccgc gcggccgatc
     3961 acgatgtgct tcgcggtggg cgacgccggg ttcgagtccc gggattgcgt aaacgtgctg
     4021 tccgcgtcca tgcaccacag gtcgttctcc tcgaagcgat ccggcggcag gcgcgacacg
     4081 tagccccgga cgacggccag ctcgtagcgg cccggggtgg agtagaactg gatgaactcc
     4141 gggtgcgacg agaaggaatg cgtgaaggcc gccacgacct gcgggaggcg aaccccgttc
     4201 gagaagaaga actggccgtt gcagatccac tgggtccccg ggaagatgat ccgtgaggtc
     4261 ggcgagacgc agtagaggcc ggagcacgtg ttgtactcca cggaatccgc gaagtaggtg
     4321 aacgcggccg aggtgtcgtt gaacacggtg gacgtcacct ggaacgagat cggctgcgtc
     4381 gaatccgaga tcgcgaagct gagccaatcg accgggaggt tgacgcgcgg caccgtgaaa
     4441 tcgaccggtt cgaagtacag tgccgggttg aagtacagaa ccggaaggtt cgcgatcacg
     4501 cgagggccgc cgggccccgt ggcgacgacg ggagcagggg ccgccgagtc cggctcgaac
     4561 agatcggtgt ccaccacggc cacgggaacc gcgacggtgt cgctgccacc gggggcgaag
     4621 tacaagggcc tcccggtcga agcgggatat ggcacccacc aggcttcgga gctgaggccg
     4681 tccgcgttgc cctgcaggga gaagcgatcg ggaaagaggt cggcaccgag cgtgagagcg
     4741 aacagctggt tcacgcccac accgtagacg gtgaccttct caccgtagtg caccacgttg
     4801 ggccggacgc tgtcgatggc gaaccgtccc gccacgcgca ccgtcgccga cccgctcgac
     4861 gcctgctcgt acgccatggg cgccgccgtg agctgcacga acccgggctt cagcccggtg
     4921 acgatggccc acccgttggg cttaccctcc accgtcgcga tcgacggatc gctcggcgac
     4981 cactggaatc cggcgttcgc gaccggctgc gtgtctcccg tggcgccgac cctgaacgcg
     5041 agcgcgtaca ccgagtcgcg cagcccctgc ccgaggatgc cgcgcgattg cagggaatcc
     5101 gagagccgaa ccacgacctg gatgtccttg gctttgtcgg tggggacgga acaggaggcg
     5161 agcacgaccg cccacgcagc gccgagcccg agggcgcgaa cgggaaaacc catgatcgag
     5221 agatccgcgg gaattgaggg catcaacata caggacaacg ggtttcgtcg ccagcggcat
     5281 tacagccgac cgcgtcgctt gagctccagg tacttgtcca cgaccgcttc ggccgcacgt
     5341 gccgggggaa cgtcgagcac gatcacgccg gcacgccgca tgtgcgacag cgcttcctca
     5401 cgcgagcgca gcagctcttc cgccgccgcc ttgcgccagg cgtcgcgcgc ttgctcgggc
     5461 cgcagcgcgg ccaacccgtc gagctccgga ttgcgcagcg tcaccgccag cggcaggtgc
     5521 cgcgggcgca gcgtgccgag gttcgcgacc agtgcttcgg aagcgaagcg gtcgatgacg
     5581 tccgtgaaca cgaccgtgag cgcgcgcttg cggttgcgcg ccgcgaggta gcggaacgcg
     5641 cccggatagt cgggctccac cagcgcgggc gtcacctcgg ccaggacgtc gagcacctgc
     5701 ttcaggccac gccgcccgcg ctgcggcgtc acgaagtgct tcacgccgtc ggcgaacacc
     5761 atcacgccga cgttgtcgtc gtgctgcgcc gccgcgtacg ccagctcgag cgccgcctgc
     5821 accacgtagt ccatgcgcgc caccccagcc acgtccgcgg tgagcagccg cccggcatcg
     5881 agcaccagca gcacctgctg gcgccgctcg gcctcgaact ggcgcgtgat cagcttgcgg
     5941 cgccgcgcgg tagccttcca gtcgatcagc cgggggtcgt cacccgggac gaagtcccgc
     6001 agcgactcga acagccgccc ttcaccgagc cggcgcaacg gctggcggcc ctgctcacga
     6061 cggcgcagcg cctgcgccac cgcggcgcgt cgtcgcaccg acgtgagcgc gggatacacg
     6121 cgcgcatccc acggcagggc gaggcggctg cgccgcacgc ccagtcccaa cggtcccccg
     6181 ctgtccaccg cgaagccgcc ggcgatctcg tggccgcgac gcaccgggac gaccggcagc
     6241 acctcccgca tgtccccgtc cgccggcacc cggacctccc gcggctcgag cgtgccgccc
     6301 agcacgtcgg gccggatttc ccgcacgcgc aaccacccgc ggcgggaccc ggcgttgcgc
     6361 cagcggtacg ccacctcccc cgcccgcccg agggaaaagg ccggaggcgc ctcacgcgaa
     6421 accgtgatct cacggtgcgc gatgcgcggc gcgagcagcg cgtcgagcca cacgccggcc
     6481 accagcatcg cgtcggccag gagcagcagg tcgagggcgc ccggaaaccg gaacccgagc
     6541 agcgcgatcc cggcgctgca ggcgagcagg acgagcgccc gccggctcca cagcacgccg
     6601 gcgtaggcgc cgacgaggta ccacagcccg acgaatccgc ccgcgactac ggtgaacagg
     6661 agcagatcga ccagatcgcc ccggctcacg cggtcggcac ctcggtcttg tcgaggatcg
     6721 ccttgagcgc ggcatcgggc gtgacgcctt ccagctccag ctcgggtgcg acgctcaccc
     6781 ggtggcgcag cacggagggc gcgagggtct tcacgtcgtc ggggacgaca tactcccgct
     6841 cgtcgagcag cgcggccgcg cgcgccgcct tgagcagcga cacgcccgcc cgcggactgg
     6901 cgccgagggt gagcgacgcg gcttcgcgcg tcgcgcgcac caccgcggtg atgtaagccg
     6961 tgatctgggg ctccacccgc actgattcca cggcagcgcg cacttcttcc agcccccgcg
     7021 cgtcggccac ccgcgagacg ccgtacgtgg cgggacggtc cgcctcgaac ccctcgacat
     7081 agcgcgtcag gatcccctgc tccacgtccg ccgctggata gccgaactgc gccttcacca
     7141 tgaaccgatc cagctccgct tcggggagcg gatacgtgcc ctcgaactcg atcgggttct
     7201 gggtcgcgaa caccgtgaag acgggcgaca gggaatggct caccccatcc accgtcacgg
     7261 tgcgctcctg catcgcctcg agcagcgcag cctgggtctt ggcgggtgcc cggttgatct
     7321 cgtccgccag gacgaggtcg gcgaagatcg gcccgggacg gaaggtgaac tcgtgcgacc
     7381 ccgtgagcat ggacacgccg gtgatgtcgg ccggcatcag gtcgggcgtg aactggatcc
     7441 gctggaaccg cacgtcgagc gccagcgcca gcgtacgcgc gagcagggtc ttcgccgtgc
     7501 cgggtacgcc ttccagcaat acgtggccgc gggcgagcag ggcgacgagc acctcgcgcg
     7561 tcgctttctc ctggcccaac accacccgat tcagcgcttc caggatgccg cgggcgacgc
     7621 ccgcggacgc aattcctccc acacgtcctc cacggcctgg gcggccgcga gcacccgctc
     7681 gttgccaccg ggtttagtca gcgtttcctg caaccggcgt gcggccctgc ggccgcgcgc
     7741 tccgggaagt gccagctcca gcgacgccag ccactgtcgg gcgtcgccgt gcggcggctg
     7801 gccggtgcgc gacaagcggc gccgcagtcc cgatatcgtg agcgccaccg ccgtatcgac
     7861 gccccccgcc ccctcgagcc cggccgcgag tgcctccacg tgctccagcg gtgagcggcg
     7921 acgccgctcg accacggcgc gcgcgggccc gaagcgcacg gcgcggacgg cgagccagag
     7981 caggacgacc gctgccagct ggaacagcac ccagcccacc ggtgagtgcg ccatccagcc
     8041 gaccagcgcg cccccgcgcg acgtctgctc gccgtagcca tgatggtatt cgtcccagct
     8101 gatacggccg cgcgtggcgg gcagcatcag gggaaccagc aggtcgggga cgtcggtgga
     8161 ccgccaggcg gcgttgcgaa agtaaccctc gtcggccacc agcagcgcgc tcccgccgcc
     8221 gcgaaaccgc aggcgcagca cgaccggcgc gtgatgcggc gtcgccagca gcgtatccac
     8281 gccgtcccgt tgggcggcac ggcagtcgtc gtcatcgtgg gacgggcggg cgagcctgcc
     8341 gcgcttggcc agctcggccg tccgcttggt cggtccctcg aggctgtcct ggccgaacgg
     8401 ccgcagcacc cagccaaccc gcggcagcgt cgcgagtccc tcgggcggca cgacggccgc
     8461 gctgtcgtcc gccgccaggt ccgccgagtc cggctcggcc gtgtcccagc cgaagcagcg
     8521 cgtgagcccg cctgcatccg ctaccgcgac gaccatcccg ccggcgtcga catagcgtgc
     8581 caccgcctcc cgctccgcgg attcgagcca gacctcgggc gacacgacgg cgagcacggc
     8641 ggggcggccg cgggcctgac gggcgagatc gaacagcggc gtacggcgcc ggtccaccgg
     8701 cacccccagc cgggcgagca cgtcgtatgg gccccgtccg ccggtcggac ccgtggtgta
     8761 cgtggagacg ggctcccagc gcgagacggc gcggcgctgg tggtgaccgg ccatcagggc
     8821 cacgatgagc gccacggcga gcaggacggc gagagcgatt tcagtgcggt tcgacatggt
     8881 gcagcagggt tccggctgcg gcgccgaatt cgcggtagcc gcgctcgtcc agcggttccg
     8941 ctccgaagac gtggtggtac aggcgggaga cgagtcccgc catggtcgcg cgacccgtgg
     9001 ctcccagccg ggcttcgcgc acgtactcgg ccggcgtctt cgacggatga aacgtgagag
     9061 cgtccgcccg gtcgagctcg agtagcagca cgatgaagcg gtgcgccaat gcctccgtgt
     9121 agcggccggt gcgcgccagc gattcggccc gcgccaggtg cccgcggctg tccagcagct
     9181 ccggggccgg tgcggcgccc gttcctcccg gctgctgcac ggtcacccgg tagatgcgcc
     9241 acaccgtgag gccgatgtgg gccagcagcg cgatcagggc gaggagcgcg gcccagaaca
     9301 acagcttcgc ccccagcggg tgctgggtcg cgaaggcgtc gagccacgcg cggaagcgca
     9361 tccagagatc gcgcagccag tgcagcgggc gggcggtctg cacccaatcg tactcggggc
     9421 gcgcgaacac ctcccgcacg gcgcgccgca ccgaatcggc gggcaccgtc acctgcggga
     9481 ggtcgaaccg gatcaggtgg cccccagttg ctcgctcaaa agctggaggt cgaacccctc
     9541 acgccgcaca cgcaagtcgt agtaggcgag agtcaggatg cacgggagga tcggcgccag
     9601 catgatcggc agcaccgaac ccacgatggt ccagtacagc tgcgccccgg ggatcagcgc
     9661 caccacgagg gcgccgatcg cctgcacgac gatcgcgggc agcaggctgc tgatgagcca
     9721 cccgaccagc accagaccca gcatccggag tttggcgccg cgggtgagct cccacgaccg
     9781 cccgaacgag tcgaacgcgc tcgagaggcg ttcgagcacg accgccggag tcgtgaccat
     9841 gtacccgcag gcgatgaaca gcaccgccca gatcgagccg acgaagagtc ccaggatcgc
     9901 cagggccatg atcgcggtgc ccagaccttt cgccaccgcg acgacgatgg cacccacgaa
     9961 caggcacacc attcccacca gcatgagcaa caggcctttg ccgaggccga cgagaaacag
    10021 ggggacgatc ttcccgaagc cgaacctcca caccgctccg aactccggct cacggcccag
    10081 gtaggagtcg gagatgatgc gaatcgaacc cgcggtcaac agcagcatgg cgcacaggta
    10141 gaagatcgcg aagaccagag tccacagcac gaggggaacg atctggtcct ggaggacggc
    10201 gacggtctcc tcgggcgtcg tggcttccgc gccgaagaac cgcacccgcc aatagagacc
    10261 gagcacgacc ggcaaccaca gcaccgcgag gctgagcttc agaaagagcg ggaagtggcg
    10321 ccggaacagc gacagggcgc cgtcgaggat ctcgccgaat tccatctgct ctatgggggg
    10381 caggtcggcc atcgggtctc gccctcacaa ggggaatggc ggtaagatga ggacgtgagc
    10441 gcccgtacgc gagccccgct gcagccgtcc ggcctgcgcc agcatctcgg cgtcgagacg
    10501 ccggagcaca tcctgctgtc cttcgagctc gccgggctgg gctcgcgagc cgcggccgcc
    10561 ttcgcggacc tggtggtgct cagcgtcgcc tacttcatca tcttcgtctt ctacgaagca
    10621 gccggactgt ccgaatcgtg ggactccggc ctcggcagct ggggcacggc actcgtcgtg
    10681 ctcgtggcca gtctgggctt cttcctttac ttcgccctgt tcgaagcgct gtgggagggc
    10741 cgcacaccgg gcaagcgtgc cctcggcctc cgggtggttc tggacaccgg tcgccccgtg
    10801 acggcgaacg cagccaccgt ccgcaacctc gtgcggatcg tggacttctt tttcccgctg
    10861 ggaccgctcc ccggcctggc gttcgtcttc tttcatcctg ccaacaagcg actcggcgac
    10921 ctcgccgccg gcaccatcgt ggtgcgcgac cgcgtggccg cctggcgact ggcggcggca
    10981 ccgccggccc cggccaacga ggcgctcgag ctcgggccgc ccgagctgag cgacccggag
    11041 ttcgcgctgc tcgaccgctt cctcgcccgg gtcgaccagc tgctgcccgc cgtccgcgac
    11101 cgcatggccg cggacctgtc gcgccgcatc gaggcgaaga tcccgcgccg cgccagcgat
    11161 cccctcgcct acctcgcgct ggtgttcgcc gacgagcagc agaaacgtcg gggccggttc
    11221 gccacgcggg cgaccggggc cgccggccgc atcaccgtca ccgccgagcg gttcttcgcc
    11281 cgcaagcgcg atacctggga agcgttccgc gaggcggcgc agcgctacga gcgcggggga
    11341 ctcgcgcagg tcccggcagc ggagatcccg gcgttcgccg cccgttaccg cgaggtggcg
    11401 gccgacctgg cccgggcgcg cacctacggc gtggctccgc aggtcgtggc ctatctcgag
    11461 cggctggtcg gggcggggca cagcgtgctg taccaggctc gcgccacgcg tcggccgccg
    11521 ttcttccgca ccgtcctggc cgatttcccg gcggccgtcg tccagtcgtg ggtgtacgtg
    11581 ctcgcgagct tcctcctgtt catgattccg gcggtcatcg gctacgtgat gctgcgcgag
    11641 cggccgcggc tcgccgacga gatcgtctcg cccgtcatgg tgagccgggc cgagcaggcg
    11701 gcggaacggg cggaggaagg gatcggctac gcgcaggccg agagcggcga gcgccccgtg
    11761 atcgcggcgg cgatcatctc gaacaacgtc cgggtgtgca tcatggcgtt cgcggggggc
    11821 atgctggccg gcatcctcac cctctgggca ctggtgttca acgggctcgc catcgggacc
    11881 ggattcggcg tgttcgccaa ttaccacgcg gcggggtatc tcgccacctt cgtggccggc
    11941 cacggcgtcc tcgagctcac cgcgatcttc atctccgggg gcgccgggtt ccgcctggcc
    12001 cacgcgttga tcgccccggg cgaccgcacc cggctcgacg cactggtcgt ggacggccgg
    12061 gtcgcgatgc gcatgatcgg cgccgtggtg tttctc
//
LOCUS       DBNGZW010000749         3971 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_125953, whole genome shotgun sequence.
ACCESSION   DBNGZW010000749 DBNGZW010000000
VERSION     DBNGZW010000749.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3971)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3971)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3971
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_125953"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            237..1040
                     /locus_tag="AC16JC_17535"
     CDS             237..1040
                     /locus_tag="AC16JC_17535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166559.1"
                     /translation="MLLRRACLIGGWMIVAASQAPAQGTAAKLACDTNLLQLAAPVNG
                     YRPRGDRCEGVYARQVAGTTLFLSSFTETFEKYDLDADDPLIIRWAAPADSGIQLRAE
                     TIKAGRYYRMDTRSAPRDSVYRWPNRILSSERLARSDLGVLGWTRITLGGRRRVAYVP
                     LAVTQKSSPTACGPLQVAFSTETRLTEVFVSLALVDSTGAETRVIKKDEPLGRGFYPA
                     GSPILISLKRADLAAAGLYSFKVSATLSPRGNYSHEYLLLIPTATACPT"
     gene            1037..2989
                     /locus_tag="AC16JC_17540"
     CDS             1037..2989
                     /locus_tag="AC16JC_17540"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF019334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5166560.1"
                     /translation="MSRSCTTCSRPIPDDSVVCPLCGADLPTSLMVGPVPTQTLGTDP
                     VREKLQAALGSGFELGARLGEGGFGVVYRARDVRLRRDVAVKLLRRELVAGEGFVERF
                     EREAQSLAALRHPNIVPVYAIGDQGELIYLIMPFVEGVTLTDYLREHGRLALDEAARI
                     LRAVGGALTAAHGVGLVHRDIKPDNIMLEGPKRHPLLMDFGIAKTGQTGGGSGVSLTS
                     TGMVLGTPLYMSPEQAKADPAVDARSDIYSLGVLAYQLFTGELPFNGDSIFAVLNQHL
                     TTPVPEPRTLRPEIPAQVSAALRRAMAKQPAERFQHVEEFIEALEGRGRTALPRPWAL
                     PGSRTLVTALLGGVLLALGAFVLWKGSQPVARAPVHAELRAQGVWFRLGGASAPWDRA
                     LTLGSLGVSGLEEVSLPIQGKNPARTVATPTLFLKAVRPDSGGISIDPIRLPARSLIA
                     VRTTGSAGTVQLTIGDSLPPIPVSVSGPIVVSLPDEPADTIPFRIDQIQLAAPRGALD
                     FELGFAAAGPARPLVPLAVIGVSFQDVNRFRDEEQASDQAVSTIDSGTFGLGVGNRAA
                     RTLTKGEDLPLHGFRGAIRDVAVDSQAVALTLEGTVDSLPASLGSIPSKLQVFWSAHP
                     AASGGAGLVYLALFVLVGLYWKRGAR"
     gene            2986..>3971
                     /locus_tag="AC16JC_17545"
     CDS             2986..>3971
                     /locus_tag="AC16JC_17545"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF024758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HIR5166561.1"
                     /translation="MRAVAGLVLVTVAAAPLAGQRMSRMPVGAANLVVQVLAGGGDDE
                     EVGAGIVVAASNRIIVATAAHVVRAAQQGGSVRVVFQFARNDTVEAKVDQVDRDLDLA
                     VLSLPRDGRATVPFAFDRQGDPGALEIGAMVVPLGCPERRCWEPPVSGDRVISASSRR
                     VRFESFFVSPGSSGGALFNQQWEVVGLVTEKNTQDGIAVGMSEVADRLRKWGYTVQLG
                     PTRIPRAGYRTRLSLVGLAPTSGGLTQSGRWPSGRLAFLLRGQERIGWHLTAIRLAPQ
                     DLKVTGGMVGTSVALRAGRLGIAPFGEVGVARVNGRFNAGSYFVQGPNGPVAV"
BASE COUNT          582 a         1358 c         1392 g          639 t
ORIGIN      
        1 cacgatgtga ttgcccaggt ggttgtcagg ggaattgagc cggagcggaa tggaaactgg
       61 attctgctgg tgaggagccg gcgccacact ctgggccgca atcgacacca ttatcgacgc
      121 caccaccgga agatgaaccg cggttcgcgg cgaggcccct agcgaaccgc cgctttgccg
      181 cgcaaaatgg tcgggccatc ttgatcgcca agcaccaacc agaaggagca ggcagcatgc
      241 ttctccgacg agcgtgcctc atcgggggct ggatgattgt ggcagcgtcc caggcaccgg
      301 ctcagggaac ggcggccaaa ctcgcctgtg ataccaatct cctgcaactc gccgccccgg
      361 taaacggcta tcggccgcgg ggagaccggt gcgaaggcgt gtatgcccgt caggtggccg
      421 ggaccacgct gttcctctcc tcgttcacgg aaaccttcga gaagtacgat ctcgacgcgg
      481 acgatcctct catcatccgg tgggccgctc cggccgactc cggcatccag ctgcgcgccg
      541 agaccatcaa ggcgggtcgt tattaccgga tggatacccg ctccgcgccc cgcgactcgg
      601 tataccgctg gcccaatcgg attctcagct ccgaacgact cgctcgcagc gacctggggg
      661 tgctggggtg gacgaggatc acgctcggcg ggcggcgacg ggtcgcctat gtgccgctcg
      721 cggtcaccca gaagagctcg cccaccgcct gcgggccgct gcaggtggcc ttttccaccg
      781 aaacccggct caccgaggtg ttcgtgagcc tggccctggt ggactctacc ggcgccgaga
      841 cccgggtgat caagaaggac gagccgttgg gtcgcggatt ctatcccgca ggcagcccga
      901 tcctcatctc cctgaagcgc gccgatctgg ccgccgcggg actgtactcc ttcaaggtgt
      961 ccgccacctt gagtccccgc ggtaactact cgcacgaata cctgctgctc atccccaccg
     1021 ccaccgcatg ccccacatga gccggtcctg caccacgtgc agccgcccga tcccggacga
     1081 cagcgtggtc tgccccctgt gcggggccga cctgcccacc tctctcatgg tcggcccggt
     1141 ccccacgcag acgctcggga cggacccggt gcgcgaaaag ctgcaggccg cgctcggatc
     1201 ggggttcgag ctcggggcga gactcggcga gggaggattc ggtgtggtgt accgcgcgcg
     1261 ggatgtgcgc ctgcgccggg atgtcgccgt caagctgctg cgccgggagc tggtggccgg
     1321 cgaaggcttc gtggaacggt tcgagcggga ggcgcagtcc ctcgccgcgc tgcgccatcc
     1381 caacatcgtt ccggtctacg cgattggaga ccagggtgag ctgatctacc tcatcatgcc
     1441 gttcgtcgag ggcgtcacgc tcaccgatta tctccgcgag cacggccgcc tggccctgga
     1501 cgaggccgcc cggatcctgc gggcggtcgg cggcgccctc acggccgccc acggcgtggg
     1561 gctcgtccat cgcgacatca agccggacaa catcatgctg gaggggccga agcgtcaccc
     1621 cttgctcatg gatttcggca ttgcgaaaac cgggcagacg gggggcggaa gcggcgtgag
     1681 cctcacctcc acgggcatgg tgctgggaac gccgctctac atgagcccgg agcaggccaa
     1741 ggcggatccg gccgtggatg cgcgctcgga catctactcc ctcggcgtcc tcgcctatca
     1801 gttgttcacc ggggagctgc cgttcaacgg cgattcgatc ttcgccgtgt tgaaccagca
     1861 cctgacgaca ccggttcccg agccgcggac cctgcgaccc gaaatcccgg cgcaggtctc
     1921 ggccgccctg cgccgggcca tggcgaagca acccgccgaa cgctttcagc acgtcgagga
     1981 attcatcgag gcgctcgagg ggcgtggccg caccgccctg ccccggccat gggccctgcc
     2041 gggaagccgg acgctcgtga ccgcgctgct cgggggtgtg ctcctggcac tcggagcgtt
     2101 cgtgctctgg aagggctcgc agccggtggc gcgcgcgccc gttcatgccg agcttcgtgc
     2161 gcaaggcgtg tggttccggc tggggggcgc gtcggcgccg tgggaccgtg cgctgaccct
     2221 ggggtccctc ggggtctctg ggctcgagga agtctccctt cccatccagg gcaagaaccc
     2281 ggcgcgcact gtcgccacgc cgaccttgtt cctcaaggcg gttcgcccgg acagcggcgg
     2341 catcagcatc gacccgattc ggctcccggc ccgcagcctg atcgccgtgc ggaccaccgg
     2401 ttccgccggg acggtgcagc tgaccatcgg agactcgctc ccgcccatac cggtttcggt
     2461 gagcgggccg atcgtcgtga gcctgccgga cgagccggcg gacacgatcc cgttccgcat
     2521 cgatcagatc cagctcgcgg ctccccgcgg ggcgctcgat ttcgagctgg gcttcgcggc
     2581 cgcggggccg gcgcgtcccc tggtaccgct cgcggtcatc ggcgtgagct tccaggacgt
     2641 caatcggttc cgcgacgagg agcaggccag cgatcaggcg gtgtcgacca tcgacagcgg
     2701 cacattcgga ctcggcgtcg gcaaccgggc tgcccgtacc ctgacgaagg gggaggacct
     2761 gccgctgcac ggcttccgcg gcgcgatccg cgacgtcgcg gtcgactccc aggccgtggc
     2821 cctcacgctc gagggcaccg tcgattccct gcccgcctcg ctcggctcca ttccaagcaa
     2881 gctgcaggtc ttctggtcgg cgcatcccgc ggcctcgggc ggcgccggcc tcgtctatct
     2941 cgcactgttc gttctggtcg gactctattg gaagaggggc gcacgatgag agcggttgcc
     3001 ggtctggtgt tggtcacggt cgcggctgcc ccgctggcgg gccagcggat gtcacgcatg
     3061 ccggtggggg ccgccaacct ggtggtccag gtgctggccg gcggggggga tgacgaggag
     3121 gtcggggccg gtatcgtggt cgcggcgtcc aatcgcatca tcgtcgcgac cgcggcgcac
     3181 gtggtccgcg cggcccagca aggcggcagc gtccgggtgg tgttccagtt cgcccgcaac
     3241 gacaccgtcg aggcgaaagt cgatcaggtg gaccgcgacc tcgacctcgc ggtgctgagc
     3301 ctgccgcgtg acggacgcgc gaccgtccca ttcgcattcg accggcaagg cgaccccgga
     3361 gccctcgaga tcggtgccat ggtggtgccg ctggggtgcc ccgagcggcg ctgctgggag
     3421 ccaccggtga gcggtgaccg cgtgatcagc gcgagctcgc gccgagtgcg cttcgagtcg
     3481 ttcttcgtaa gcccggggag ctcggggggc gcgctgttca accagcagtg ggaagtggtg
     3541 gggctggtca cggaaaagaa cacccaggac ggcatcgcgg tcggcatgag tgaggtggcc
     3601 gaccggttgc ggaagtgggg ctacaccgtc cagctcggtc cgacgcgtat cccccgcgcc
     3661 gggtaccgca cccgcctcag tctggtcggg ttggcgccga cctccggcgg cctgacccaa
     3721 tcgggccggt ggccgagtgg gcgactcgcg ttcctgctcc ggggccagga gcggatcggg
     3781 tggcacctga cggcgatccg tctcgcgccg caggatctga aggtgaccgg aggcatggtg
     3841 gggaccagcg tcgcgctgcg ggccggacgg ctcgggattg ccccgttcgg cgaggtcggg
     3901 gtcgcccggg tgaacgggcg cttcaacgcc gggagctact tcgtccaggg cccgaatgga
     3961 ccggtggcgg t
//
LOCUS       DBNGZW010000750         7267 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1829407, whole genome shotgun sequence.
ACCESSION   DBNGZW010000750 DBNGZW010000000
VERSION     DBNGZW010000750.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7267)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1829407"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            260..1249
                     /locus_tag="AC16JC_17550"
     CDS             260..1249
                     /locus_tag="AC16JC_17550"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HIR5166562.1"
                     /translation="MRRVALGIGTLVVAGLGACAPARIDPRSLFLAPWRPPPGQRCSV
                     SKKPIPPAGVLFDTAALGRAVAPFGSGNVVSALAFWPADTAWHEEFGPRPDSLVIVET
                     TLPDSLRGPVRAALLGALRRRIAERLLIRIDVNREARLRLAPALECPPAVRSQADVRR
                     YAAALQSLGTPPGRAVLQFLVRPDGSLTAFKVQIPSGDLDFDRAAVSTVQLLRFTPGL
                     INHIPVPGLVRFPVEVRVTRQAPPPPLTAAECPRGRVVEIINPLPAAVLVYAFSPAGA
                     QLLGTTGADTTVRFALPPASAGYGYVQWADAAAPGASDADLKKVRWRVKCGVP"
     gene            complement(1268..2971)
                     /locus_tag="AC16JC_17555"
     CDS             complement(1268..2971)
                     /locus_tag="AC16JC_17555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166563.1"
                     /translation="MIDASGFFDAADAEALVDLRLRGLVERYARLVGATVQELEPQLV
                     ELSLPDAEIPHFNGRPSVRVAFSLAALERHPDAEMAVVGSAFLRELLDAVRTRGARRD
                     CGLIPPTHPADTNGLPRTVAVRRGMAQPPVVSLALHPVARLVARVAITAAAIVAEHIA
                     ESGVFDLVTGLRAPDDVAGLCRDIEAGTVVPAGDGAGPAAGVAALPARPLAELVPLML
                     GDIESKLATKLARHRHDAEHSLAAELARIDRYFGQLLEEAAESAWDPMSAEERRAIEA
                     EHRRRRAEEERRHDVRVSVHPIQLVEFTVLVQRVDWKLVIQGGRGATLTAQRTLSGSG
                     AWQFACPHCGAAPELLLICRHGHVACAACGMTCRVCGEEFCCAHGIAACHVDDEPACD
                     VHAKTCPTCRKPHCTRHAGTCSVGDHPACSACLGPCSLCGKVVCATHAVRSDSSAPKG
                     ERRLCLECMVYCEGKTHEPVGRDEAVRCTSCGQFVCANHQAACVVDQLVHCSTHLRRA
                     DRSRRLVCERHRGQCGHEPGVIFASDEVAPCPACGKSVCDAHAGTCVAAASVRTPGGF
                     R"
     gene            complement(2968..5028)
                     /locus_tag="AC16JC_17560"
     CDS             complement(2968..5028)
                     /locus_tag="AC16JC_17560"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR5166564.1"
                     /translation="MEGLGNGASSGEWFLLRREFEALALNPGFDRLITLDCNTIKELP
                     HQIDVALRVLRRPMGGRAILADEVGLGKTIEAGIIMKELGVRGMARRVLILAPASLVE
                     QWQAELETKFFEEFDTPTEPEDWQHATKAIASYQRAVGHAPEILQHRWDLVIVDEAHN
                     VKNHTTAVHQFLRQLDRNFMLLLTATPLQNDLRELYNLVTLLRPGQLGTWSQFQKEYV
                     QGGEPRRASNPEALREFTSQVMIRTRRSSVADVLNLPPRRPVHHAIALTPDEAALYGD
                     TVCFLRDLYREGLFKPSPPEELEDQARRRLRTAKGTFVLELMRLLQRLTSSSRALADS
                     LHSVASGDLILPTYRQRAGQLAQQARAVTDHAKLDLLTRTLADTPDRVIVFSEHLPTL
                     NLLQHRIQEVGRPVITFTGLLSRAERSQRLARFRAEPGSVFLTTRVGSEGLNLQFCNR
                     IVNYEIPWNPTIIERRIGRVLRIGQEREVHILNLAAQATIEMPVLWLLDRKIRLFDLD
                     VGGLDMVLGEFGGAETLEHRLADAWLRAESDSAFERALDAIGEQIVASREAGAQQARL
                     ASDIVAEDNAERLEREFRQLSVPGRVVLGYGTCHLKLARGVEAKRHQLGLHVTEIREA
                     AEHARHAEDAGHHPEYGNLRRIVGVTGRGREVQLLVQADRLPMTLADLSVDAEAPLAE
                     LAAP"
     gene            complement(5462..>7267)
                     /locus_tag="AC16JC_17565"
     CDS             complement(5462..>7267)
                     /locus_tag="AC16JC_17565"
                     /inference="COORDINATES: protein motif:HMM:NF025067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HIR5166565.1"
                     /translation="GRMNDLSQRLRLPTIGAAVSAAEHAARRFPLVLLSAALAATAGI
                     LLVSASDDEEPFVRLLVTATLGLPLFFALTLTGERRFAAGDAARRWGLLGAGVVVLVV
                     FWALWPQWPEPVQFARYVQASAAFHLLAAFLPFAEYDEPNGFWQYNKALFLRFLVAGI
                     YAAVLYGGLALALAALDKLFGVDVPGEAYGRLWMVMAFVVSTWLFLGGVPDDFAALER
                     RSDVPAGLRIFTQYVLVPIIAVYLVMLTVYLGKVLIDRQWPSGWIGYLVSSVATVGIL
                     AWLLVRPLEEMADYRWVRTYTRGFYVALLPAIVMLWLAIGKRVAQYGVTERRYFMIVL
                     SLWWAGIAVYYTLSRSRNIKVIPATLCLLAVLTFTGPWGAYQVSLFSQRHRLDQLLAR
                     YGLRVVATGATAREVPFADRKEIAATVRYLVERHDRKGIATRFGGPASRASSAVETRV
                     RTIMASLGVAYVSPWEREEGEESHSFRAQWTTAPTPIDGYQYAIHLGHFEPKDTLRLD
                     AASVSFAQARNALRVERGGLAALEIPLQPVIERATAYRRTRGRYDEVPIGLLRGETSG
                     PGGAALLYLTMLNASHGDSGWTVTAIDGELFLRLK"
BASE COUNT         1158 a         2524 c         2516 g         1069 t
ORIGIN      
        1 gggaagcgtt ggtggcgcac ggtgcgctgc gcgaacgaag tcctcgtagg gcatcgactt
       61 cacacccgtg tgcgcggccc agcgtggagg cacacgacgt ggacctggag ggcccagaac
      121 ccgtccccga cgcgcgcgac ctgaagcgct agtgcgcggc gtctcatcgc ggcattttga
      181 ctatgcaggg cgatcgagga gaaaccgtgt gacctgcacg agctggagag ctatcgcagg
      241 gcggaggggg ggccggctgg tgaggcgcgt cgcactgggc atcggcacgc tcgtcgtcgc
      301 gggcctgggc gcctgcgcgc ctgcccgcat cgatccccgc agcctcttcc tcgctccctg
      361 gcggcccccg cccggccaga ggtgctccgt cagcaagaag ccaattccgc cggccggtgt
      421 gctgttcgat acggccgcac tcggccgcgc cgtggccccg ttcggctcgg ggaacgtcgt
      481 gtccgccctc gccttctggc ctgcggatac ggcgtggcac gaggagttcg gcccacgccc
      541 ggacagtctg gtcatcgtcg agacgacgct gccggactcg ttgcggggcc cggtccgcgc
      601 cgccctactc ggagcgctgc ggcggcggat cgccgagcgg ctgctcatcc ggatcgatgt
      661 gaaccgggag gcgcgcctgc gactcgcgcc ggcgctcgag tgcccgccgg cggtgcgcag
      721 ccaggcggat gtccgccgct acgcggcggc gctgcagtcc ctcggcaccc cgcccggccg
      781 agcggtgttg cagttcctcg tccgaccgga tgggtcgctc acggcgttca aggtgcagat
      841 tccctcgggc gatctggact tcgatcgggc ggccgtgagc actgtgcagc tcctgcgctt
      901 caccccggga ctgatcaatc acattccggt ccccggcctg gtgcggtttc ccgtcgaggt
      961 gcgcgtcacg cgccaggcgc cgccgccccc actcacggcg gccgagtgcc cgcgggggcg
     1021 cgtggtcgag atcatcaatc ccctcccggc ggccgtcctg gtctacgcgt tcagcccggc
     1081 gggcgcgcaa ttgctcggca cgacgggggc ggacacgacg gtgcgcttcg ccctgccgcc
     1141 cgccagtgcc gggtatgggt acgtgcagtg ggccgacgcg gccgctccgg gggcttcgga
     1201 cgccgatttg aagaaggtgc gctggagggt gaaatgcggg gtgccgtaac ccattccctc
     1261 ttgattctca cctgaaacct ccgggtgtcc ttacagaagc agctgccacg caggtcccgg
     1321 cgtgcgcgtc gcatacggac ttcccgcatg ccggacacgg tgccacctcg tcgctcgcaa
     1381 agatgacgcc cggctcgtgg ccgcactggc cgcggtgccg ctcgcacacc aggcggcgcg
     1441 accgatccgc acggcggagg tgcgtggagc aatggacgag ctggtccacg acgcacgcgg
     1501 cctggtgatt ggcgcacacg aactgcccgc agctggtgca gcggacggct tcgtcccggc
     1561 cgacgggctc gtgggtcttg ccctcgcagt acaccatgca ttcaaggcag agacgacgct
     1621 cgcccttggg ggcgctggag tcactgcgaa ccgcatgcgt ggcgcacacc accttgccgc
     1681 agaggctgca cggaccgagg caggcgctgc acgccggatg gtccccgacg ctgcacgtgc
     1741 cggcgtgcct ggtgcaatgc ggcttgcgac acgtggggca ggtcttcgca tgcacgtcgc
     1801 aggcgggctc gtcgtcgacg tgacacgcgg cgatgccgtg cgcgcagcag aactcctcgc
     1861 cgcacacccg gcacgtcatc ccgcaggcgg cacaggccac gtgaccgtgg cggcagatga
     1921 gcagcaattc cggggcggct ccacagtgcg ggcaggcgaa ctgccaggcg ccggagccgc
     1981 tcagcgtccg ctgcgccgtg agcgttgccc cccgcccgcc ctggatgacg agtttccagt
     2041 ccacacgctg cacgagcacc gtgaactcca cgagctgaat cggatggacg gacacgcgaa
     2101 cgtcgtggcg gcgctcctcc tcggcccgcc ggcggcggtg ttcagcctcg atggcgcgac
     2161 gttcctccgc gctcatcgga tcccaagccg actcggcagc ctcctcgagc agttgcccga
     2221 agtagcgatc gatccgggcc agctcggcgg ccagggagtg ttcggcgtca tgtcggtgtc
     2281 gcgcgagctt cgtcgccagt ttggactcga tatcaccgag catgagcgga accagctctg
     2341 cgagcggccg ggccggcagc gccgcgaccc cggcggcggg ccccgccccg tcgccggccg
     2401 gcacgacggt gccagcctcg atgtcgcggc acagtcccgc cacgtcgtcc ggtgcgcgca
     2461 agcccgtgac gaggtcgaac actccgcttt cggcgatgtg ctctgcgacg atcgccgccg
     2521 ccgtgatcgc cacccgcgcg acgagccgag ccacggggtg cagcgcgagg ctgacgacag
     2581 gcggctgcgc catgcctcgg cgaaccgcaa ccgtgcgcgg caggccgttg gtgtcggcag
     2641 ggtgagtcgg cggaatcagc ccgcagtcgc gtcgagcgcc ccgcgtgcgg accgcgtcga
     2701 gcaactcccg aagaaacgcg ctgccgacga cggccatttc ggcgtcgggg tgacgctcga
     2761 gcgccgcgag cgaaaacgca acgcgcaccg aggggcgccc attgaagtgc gggatctcgg
     2821 catccgggag cgacagctcg accagctgcg gttccagctc ctgaaccgtc gcaccgacca
     2881 gacgcgcgta ccgctccacc agcccccgca ggcgaaggtc gacgagcgcc tccgcatccg
     2941 ccgcatcgaa gaaccctgat gcgtcaatca tggagccgcc agctcggcga gcggagcctc
     3001 ggcatcgacg gacagatcgg cgagggtcat gggcaggcgg tcggcctgca ccagcagctg
     3061 tacctcccgg ccgcgcccgg tgacgcccac gatgcggcga aggttcccgt attccggatg
     3121 atgacccgcg tcctcggcgt gcctggcgtg ctcggcggct tcgcgaatct cggtgacatg
     3181 caggccgagc tgatggcgct tcgcctccac gccgcgcgcg agcttgagat ggcaggtgcc
     3241 gtagcccagc acgacccgtc cgggaaccga aagctgccga aactcccgct ccagccgctc
     3301 ggcgttatcc tcggccacga tgtcggacgc cagccgcgcc tgctgcgccc cggcctcccg
     3361 actcgccacg atctgctcgc cgatagcatc gagcgcccgc tcgaaggccg aatcggactc
     3421 ggcccgcagc catgcatcgg ccaggcgatg ctcgagggtc tcggcgccgc cgaactcccc
     3481 caggaccatg tcgagcccac ccacgtcgag atcgaacagc ctgatcttcc gatcgagcag
     3541 ccacaggacg ggcatctcga tcgtggcttg tgcggccagg ttcaggatgt gcacctcccg
     3601 ctcctggccg atgcgcagca cccgaccgat ccgccgctcg atgatcgtgg gattccacgg
     3661 gatctcgtag ttcacgatcc ggttgcagaa ctgcaggttc aggccttcgc tcccgacgcg
     3721 ggtcgtgaga aacacgctcc ccggctccgc ccggaagcgg gccagccgct ggctgcgctc
     3781 ggcgcgggaa agcaacccgg tgaaggtgat cacgggccgc ccgacttcct ggatccgatg
     3841 ctggagcagg ttgagcgtcg ggaggtgctc gctgaagacg atcacccggt ccggcgtgtc
     3901 ggcgagggtg cgggtcagca ggtccagctt ggcgtgatcg gtcaccgccc tcgcctgctg
     3961 cgcaagctga ccggcccgct gccggtacgt cggcagtatc aggtctcccg aagccacgga
     4021 gtgcagcgag tcggcgagcg ccctggacga cgacgtgagc cgctgcagca ggcgcatcag
     4081 ctccaggacg aaggtgccct tggccgtgcg gagccggcgc cgggcctggt cctcgagttc
     4141 ctccggtgga gacggcttga aaaggccttc ccggtagaga tcgcgcagga agcacaccgt
     4201 atccccgtac agggcggctt cgtcgggcgt cagggcgatg gcgtggtgga cggggcgccg
     4261 cgggggcagg ttgagcacgt cggccacgct cgagcgccgt gtgcggatca tcacctggct
     4321 cgtaaactcg cgcagcgcct cggggttgct tgcccggcga ggttcgccgc cctggacgta
     4381 ctccttctgg aactgactcc aggtgccgag ctggccgggc ctgagcaatg tgacgaggtt
     4441 gtacagctcg cgcaggtcgt tctgcagcgg cgtggcggtg agcagcagca tgaagttccg
     4501 atccagctgc cgcaagaact ggtgcaccgc cgtggtgtgg ttcttcacgt tgtgggcctc
     4561 gtccacgatc acgaggtccc agcgatgctg gagtatctcg ggcgcgtgcc cgacggcgcg
     4621 ttgataggac gcgatcgctt tggtcgcgtg ctgccagtcc tccggctcgg tgggcgtgtc
     4681 gaactcctcg aagaacttcg tctcgagctc ggcttgccac tgttccacga gggacgcggg
     4741 agcgaggatc aggacgcggc gcgccatgcc gcgcacgccc agctccttca tgatgatccc
     4801 ggcttcgatg gtcttgccga gaccgacctc gtcggcgagg atcgcccgcc cgcccatggg
     4861 ccggcgcagg acgcgcaggg cgacgtcgat ctggtgcggt aactccttga tggtgttgca
     4921 gtccagcgtg atgagccgat cgaagcccgg gttgagcgcg agggcctcga actcacggcg
     4981 gaggagaaac cactctcctg aggaggcccc gttcccgagc ccctccatca agcagcgcag
     5041 cgactcctgg tcgatctccc agtggccgag gcgtgcagct gtcggcggcg tgggcggagc
     5101 ccctgggtcc aaacagcggg tcggcaacgg ctacctcgtc cctagatgga atcggtgggc
     5161 aacgtcgacc caagtagcag attccgtgcc tcaggttgcg ccggccgcat ttgcagcagt
     5221 agcaggcccg ggaagaaaga gccccacctc gaaactcgct gcagagcttg atcttcaagc
     5281 agtcgggacg gccggatttg agcggaaagc cgcgagccag gtgtcgcgaa gccacctccg
     5341 aaccccgcga tggaagtacc gaccccgtga aggaagctgc aggaaacggt cgcggatgcg
     5401 ggagagccgc tgcggctgcg actctatggc ggcagcttta tcgagatccc gatcgcaacg
     5461 actacttcag ccggaggaac aactcgccgt cgatcgccgt gaccgtccag ccgctatcgc
     5521 catggctggc gttgagcatg gtcagataca ggagcgcggc gccgcccggg ccgctcgtct
     5581 ccccgcgcag caggccgatc ggcacctcgt cgtagcgccc gcgcgttcgc cggtaggcgg
     5641 tggcgcgctc gatgacgggc tggagcggaa tctcgagcgc cgcgagtcca ccgcgttcca
     5701 cccgcaacgc attacgcgcc tgtgcgaacg agacactggc ggcgtcgagc cgcagggtgt
     5761 ccttcggctc gaagtggccg aggtggatcg cgtactggta accgtcgatg ggggtcgggg
     5821 ccgtggtcca ttgggcgcgg aaggagtggg actcctcgcc ttcctcacgc tcccacggcg
     5881 agacgtacgc gacgccgagg gaggccatga tcgtccgcac ccgtgtctcg accgccgagg
     5941 aagcgcggga cgccggcccg ccgaagcggg tggcgatccc tttccggtcg tgacgctcca
     6001 cgagataccg caccgtcgcg gcgatctctt tgcggtcggc aaaggggacc tcccgcgccg
     6061 tggctcccgt tgccacgact cgcaggccgt acctggccag gagctggtcg agccggtgcc
     6121 gctggctgaa gagtgagacc tggtaggctc cccacggacc ggtgaacgtc aacaccgcga
     6181 ggaggcagag cgtcgcggga atcaccttga tgttgcgcga gcggctcagc gtgtagtaca
     6241 cggcgatccc cgcccaccac agcgacagga caatcatgaa atagcgccgc tcggtgactc
     6301 cgtactgggc gacccgcttg ccgatcgcga gccacaacat cacgatcgcc ggcaacagtg
     6361 ccacgtagaa gccgcgcgtg taggtccgca cccagcgata gtccgccatt tcctcgagcg
     6421 gccgtaccag cagccacgcg aggatcccga ccgtcgcgac gctcgagacg agatatccga
     6481 tccacccgct cggccactgg cggtcgatca acaccttgcc gaggtacacg gtgagcatca
     6541 cgaggtagac cgcgatgatc gggacgagga cgtactgggt gaagatccgc agtcccgccg
     6601 gcacgtcgct gcggcgctcg agcgcggcga agtcgtccgg cacgccgccc aggaagagcc
     6661 acgtcgagac gacgaaggcc atgaccatcc acagccgccc gtacgcctcg cccgggacgt
     6721 ccacaccgaa cagtttgtcc agcgccgcca gggccagcgc caggccgccg tacaacaccg
     6781 ccgcgtagat cccggccacg aggaagcgca ggaagagcgc cttgttgtac tgccagaaac
     6841 cgttgggctc gtcgtactcg gcgaacggca ggaacgccgc cagcaggtgg aacgcggcgc
     6901 tcgcctgcac gtagcgcgcg aactggaccg gctccggcca ctgcggccac agcgcccaga
     6961 acaccacgag caccacgacc ccggcgccca gcaaccccca acgccgtgcg gcgtcgccgg
     7021 ccgcgaagcg ccgctcgccc gtgagcgtga gcgcgaagaa cagcggcagc ccgagcgtcg
     7081 cggtgacgag gaggcggacg aacggctctt cgtcgtccga ggcgctcacg aggaggatgc
     7141 ccgcggtcgc cgcgagggct gccgagagga gcacgagggg gaatcgccgc gccgcgtgct
     7201 cggcggccga gacggctgcg ccgatcgtcg gcaggcggag tcgctgggag agatcgttca
     7261 tccggcc
//
LOCUS       DBNGZW010000751         3437 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1079672, whole genome shotgun sequence.
ACCESSION   DBNGZW010000751 DBNGZW010000000
VERSION     DBNGZW010000751.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1079672"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..2599)
                     /locus_tag="AC16JC_17570"
     CDS             complement(59..2599)
                     /locus_tag="AC16JC_17570"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5166566.1"
                     /translation="MTASFLLAMALALQTRIPCPRRAGAITESAWQAYRANRLALAAS
                     RFAHADSLCPGIAEVNAGLGFVALRRGSSDEAQRRFDAALRADSGNADAWYGLGLARA
                     REGQRDSAIAALRRALAIAPGYPGAQTQLTLLGDLGPPLEQPSRPGTVQMPARTHGAR
                     FEILQDGAWRPFYVKGINLGAARPGKYPSEFPTDDSTYAHWLSLMAAAHANVVRVYTI
                     LPPAFYNALKGWNDSHPDQALWLVHGVWAEAPPNDDYDDREWKLDFRAETQRVVDLLH
                     GHALIPPRPGHASGRYTADVSRHVLAFIIGREWEPFTLRHYNSLRHGRTAYRGTFFTL
                     PRGTPADVWMVEQCDYLMRYEWDTWHAQRPIAYSNWPTLDPLSHRTEPTVEEESALRE
                     RIGLPPLTNRREYDNDDQTLDAMLVHPTARNRAGYFASYHVYPYYPDFMIYDSTYGTA
                     RSSYGPSHYYGYLRALTRYHAGRPLLVSEYGVPSSRGLGHLHPDGMDHGGHDEAEMAA
                     IDVRLTREIREAGAAGGILFAWIDEWFKHNWSVVDFEVPPERRRLWHNAMDPEQYYGL
                     LGMDAGKDTAPPAPGGDPARWRALPLLAGHNRSRLRVGSDESYLYLALESSGGTRRSA
                     RTLIGIDTYARDRGEFRLPDGQRTETGVEFYLDVSDTAAGRLFIARRYSPYITPRPGM
                     GPTDLDPFYNVECTVDVRSDSADFDSLFAVANRFRVTRSLRQIPVRGVDRGRLRYGRA
                     SASTLSDWFVDRDAGLIEIRLAWGLLNVTDPSSRTVLTAVRHDNSVAADTTDGFRFFV
                     AEPPLGAADPEALLTASATYRWPTWEVPTWHERLKPAYFALRDLWGSW"
     gene            2732..2926
                     /locus_tag="AC16JC_17575"
     CDS             2732..2926
                     /locus_tag="AC16JC_17575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166567.1"
                     /translation="MQADVGLCYSCRWMRPVTNRRGSTFYRCARAETDVRFVRYPPLP
                     VLNCSGYEKETEKGVGSGER"
     gene            complement(2972..>3437)
                     /locus_tag="AC16JC_17580"
     CDS             complement(2972..>3437)
                     /locus_tag="AC16JC_17580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166568.1"
                     /translation="QPLSPGGVVMQRVAVAIAVGVAVLAPSARAQDNQWRTQVEGYLK
                     RAAQSLNERGFSQTHETQIGSLADDDDDSFTLTLHAGTAYALVGVCDNDCKDLDLVLY
                     DADGNQVDSDVQADDVPIVQVTPSETMRYRVKVIMATCNTNPCWYGVGAYGK"
BASE COUNT          520 a         1105 c         1277 g          535 t
ORIGIN      
        1 tgggcggccg cgggcccggt catcgcgacg aggaccgaga aggccgcggc ggcggtcctc
       61 accaggaccc ccagaggtcc cgcagcgcga agtacgccgg cttgaggcgc tcgtgccacg
      121 tgggcacctc ccaggtgggc caccggtagg tggcgctcgc cgtcaggagc gcctcggggt
      181 cggcagcccc gagcggcggc tcggccacga agaaccggaa gccgtcggtc gtgtcggctg
      241 cgaccgagtt gtcgtggcgc acggcggtca gcaccgttcg cgacgacggg tcggtcacgt
      301 tgagcaggcc ccacgcgagg cggatttcga tgaggccggc gtcgcgatcc acgaaccagt
      361 ccgacagcgt ggaggccgac gcgcgcccgt agcggaggcg accgcggtcc acgccgcgca
      421 ccgggatctg ccggagggac cgggtgaccc ggaaccggtt ggccacggcg aacagggaat
      481 cgaaatcggc actgtcgctc cggacatcca ccgtgcactc gacgttgtag aacggatcga
      541 ggtcggtggg ccccatgccg ggccgtggag tgatgtacgg actgtagcgt cgggcgatga
      601 acaggcgacc cgccgccgta tcggagacgt cgaggtagaa ctccacaccg gtttcggtcc
      661 gctgcccgtc cggcagccgg aactcgccgc gatcacgagc ataggtgtcg atcccgatga
      721 gggtgcgggc cgagcggcgt gtcccgccgg acgattcgag ggcgaggtac aggtagctct
      781 cgtcggaacc gacccgcagg cgcgaccggt tgtggcccgc gagcagcggc agcgcacgcc
      841 agcgggccgg gtcgccaccg ggggccggcg gagccgtgtc ctttcccgcg tccattccca
      901 gcaatccata atactgctcg gggtccatcg cgttgtgcca cagccgccgc cgctccggtg
      961 gcacttcgaa gtcgaccacc gaccagttgt gcttgaacca ctcgtcgatc caggcgaaca
     1021 ggatcccgcc ggcggcgccc gcttcgcgaa tctcgcgcgt caggcggacg tcgatcgcgg
     1081 ccatctccgc ttcgtcgtgt cccccgtggt ccatcccatc ggggtgcaag tgaccgaggc
     1141 cgcgcgagga gggcacgccg tactccgaga cgagcagcgg ccgtccggcg tggtaccggg
     1201 tcagcgcgcg cagatatccg tagtagtgcg aggggccgta gctggagcgg gccgtgccgt
     1261 acgtcgagtc atagatcatg aaatccgggt agtacgggta gacgtggtag gacgcgaagt
     1321 acccggcgcg attcctggcg gtcgggtgca ccagcatggc gtcgagcgtc tggtcgtcgt
     1381 tgtcgtattc ccgccggttg gtgagcggcg gcagcccgat gcgctcgcgc agcgcgctct
     1441 cctcttcgac cgtcggttcg gtgcggtggc tcaacggatc gagcgtcggc cagttcgagt
     1501 aggcgatggg gcgctgggcg tgccaggtgt cccactcgta gcgcatcagg tagtcacact
     1561 gctccaccat ccagacgtcc gccggcgtgc cgcgcggcag tgtgaagaac gtgccgcgat
     1621 acgcggtcct gccgtgccgc aggctgttgt agtggcggag cgtgaacggc tcccactctc
     1681 ttccgatgat gaacgccagc acgtggcggg agacatctgc ggtatagcgg ccggatgcgt
     1741 gcccggggcg gggggggatc agggcgtggc cgtgcagcag atccacgacc cgctgcgtct
     1801 ccgcgcggaa gtccaatttc cattcgcggt cgtcgtagtc gtcgttcggc ggcgcttcgg
     1861 cccagacgcc gtgcaccagc cacagcgcct gatcggggtg cgagtcgttc cagcccttca
     1921 gggcgttgta gaatgccggc ggcaggatcg tgtagacacg gacgacgttg gcgtgggcgg
     1981 ccgccatcag gctcagccag tgcgcgtagg tggagtcgtc ggtggggaac tccgacgggt
     2041 atttccccgg ccgggcggct ccgaggttga tgcccttcac gtagaagggg cgccaggcgc
     2101 cgtcctggag gatctcgaag cgcgcgccgt gggtacgcgc aggcatctgg acggtgccgg
     2161 ggcgcgacgg ctgctcgagt gggggaccga ggtcgccgag cagcgtgagt tgggtttggg
     2221 cgcccgggta gccgggcgca atcgcgagcg cgcggcgcaa ggccgcgatc gccgagtcgc
     2281 gctgcccttc ccgggcgcgg gcgaggccca ggccgtacca ggcgtcggcg ttccccgagt
     2341 ccgcccgcaa cgccgcgtcg aagcgccgct gcgcctcgtc ggacgatcca cgccgcagcg
     2401 cgacgaagcc gaggccggcg ttcacttcgg cgatgccggg gcagagcgag tcggcgtgcg
     2461 cgaagcggct ggcggcgagc gcgaggcgat tcgcgcgata ggcctgccag gcgctctcgg
     2521 tgatcgcgcc ggctcgccgg gggcagggga tgcgcgtctg cagggcaagc gccatcgcaa
     2581 gcaggaagga ggccgtcacg attcagcaag ataggcggct gcatgggggt gcggccatgg
     2641 ccgacacgac tgggagtggg gagtggggag cggtactatc cggtgcatcg ggatcgactg
     2701 ctctgtgctc cgtgtcgact tggggacggc catgcaagca gacgtcggac tctgttacag
     2761 ctgccgctgg atgcgtccgg tgacgaatcg ccgcggctcg acgttctacc gctgcgcccg
     2821 cgccgagacg gatgtgcgct tcgtgcgcta tccgccgctg ccagtcctca attgctcggg
     2881 atacgagaag gagacggaaa agggagtggg gagcggggag cggtaatgca ccggatcaga
     2941 ccactccccg ctccccactc ccgctcgtgg actacttccc gtacgccccg accccgtacc
     3001 agcagggatt ggtgttgcag gtggccatga tcaccttgac gcgataccgc atcgtctcgg
     3061 agggcgtcac ctggacgatc ggcacatcgt cggcctggac gtcggagtcg acctggttgc
     3121 cgtccgcatc gtagaggacg aggtcgaggt ccttgcagtc gttgtcgcag acgcccacca
     3181 gcgcgtacgc cgtgccggcg tgcagcgtga gcgtgaagga atcgtcgtcg tcgtcggcca
     3241 acgagccgat ctgcgtctcg tgggtctgcg agaagccccg ttcgttgagc gactgggcgg
     3301 cgcgcttcag gtacccctcg acctgcgtgc gccactggtt gtcctgggcg cgggcgctgg
     3361 gtgccaggac ggcgaccccg accgcgatgg cgacggcgac tcgctgcatg acgactcctc
     3421 ctggggaaag cggttgg
//
LOCUS       DBNGZW010000752         4404 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1079830, whole genome shotgun sequence.
ACCESSION   DBNGZW010000752 DBNGZW010000000
VERSION     DBNGZW010000752.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4404)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4404)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4404
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1079830"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="AC16JC_17585"
     CDS             complement(<1..371)
                     /locus_tag="AC16JC_17585"
                     /inference="COORDINATES: protein motif:HMM:NF024313.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5166569.1"
                     /translation="MQAPRHWRRFARDRWALTGLVLVTILAVLALTAPWIAPADPLHG
                     SLSVSLRAPSRAFWLGTDVQGRDVLSRVLFGARLSLLVGLVSQTVASVLGVGLGLAAG
                     YYGRWVDASIMRLADVTLAFPS"
     gene            438..1523
                     /locus_tag="AC16JC_17590"
     CDS             438..1523
                     /locus_tag="AC16JC_17590"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR5166570.1"
                     /translation="MNPGTRFTLVLGGGGMKGLAHVGVLEALLERGLKPSHIVGCSVG
                     SLVGAAWSSGKSIPELRDIAINLKRKDVFQIAHADMAFKRMRSPALFRREPLALLIDR
                     LVGDAAFADLDVPVICNTVDINSGMQVLWGLPGLDDIPVGEAVFGSCALPGYLPPHEI
                     RGRHFVDGAVIENLPIAAARALDSEMVFAVDVSASSTLREDTQEGGFAAVFGRATEIA
                     MQALLEQRLRQWTTPPVYYIHPRVEHISMFSFDHLREVVEEGYRATTEALERADTWPA
                     RGAEGVYPRRRVIVQVARDRCIGCGACLFQGPPGLFVLDTERKAVVTQPEQEWTPASG
                     SFIRHCPTYAISARPVPAAMETMRRSG"
     gene            1766..4324
                     /locus_tag="AC16JC_17595"
     CDS             1766..4324
                     /locus_tag="AC16JC_17595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015187388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5166571.1"
                     /translation="MRIKFQHAAILTPSAPARANQKPGATALPPSPGQPPAVIPASRP
                     SVRGKFLFLGDEKLHVRGVTYGTFRPQADDSEFPTADVVERDFALMAAHGVNSLRTYT
                     VPPRWLLDSASRHGLHVLVGIPVERHIGFLTDRKGAPDTGRLVRSGVAACAGHPAVLA
                     YAIGNEIPAATARWHGAGRVERFLEKLFRAVKAEDPSGLVTYVNYPSTEYLQLPFLDF
                     VSFNVFLESRAPLAAYLARLHHVAGERPLLLTEVGLDSLRNGDAAQARTLDWQIRTSF
                     AEGCAGVFVYAWTDEWYRAGEDVTDWRFGLTSRDRSPKPALAAVQAAFADTPFPAEMR
                     WPRMSVVVCTYNGSRTIRECMEGIRRLRYPDFEVIVVNDGSTDRTAEIVREYDVRLIS
                     TDNRGLSSARNTGWREATGEIVAYIDDDAYPDPDWLTHLAATFLRTTDVGVGGPNIAP
                     PGDGPIARCVALAPGNPIHVMLTDREAEHIPGCNMAFRRDALAAIGGFDERFRIAGDD
                     VDVCWSLRERGLTLGFSPAAVVWHHRRNSVRAFWKQQVGYGRAEGLLERKWPEKYTAT
                     GQIAWAGRLYGATLLPAAPWRSGRIYHGTWGFAPFQRLYQATPTWIGSLALAPEWYLP
                     IAALAVVALLGITWAPLRLAIPLLALATAPLIMRAAIAARISFPRSRRTRRIRQRLLT
                     GYLHLLHPLARLYGRLTRPATHRARRGWALGATVALWCERWRDPGERLNAIERALRES
                     EAGAVLRGGDFERWDLEVRGGTLGAARLAMAVEEHGMGRQLVRVRWWLAPSPAGPALA
                     LLLGGLAIVAETTSVWIPGAVFGAAALWCAWRTIAACGSAAADIRRAVGRAGDG"
BASE COUNT          632 a         1586 c         1537 g          649 t
ORIGIN      
        1 gagggaaagg cgagggtgac gtcggcgagc cgcatgatgg acgcgtcgac ccagcgcccg
       61 tagtagcccg cggcgaggcc gagcccgacc cccagcacgc tggccaccgt ctggctcacg
      121 agcccgacca gcagcgacag gcgagcgccg aacaggacac gtgacaaaac gtcacgcccc
      181 tgcacgtcgg tgccgagcca gaaggcgcgc gacggggcgc gcaggctgac ggacagggag
      241 ccgtgcaacg ggtcggccgg cgcgatccac ggcgcggtca gcgccagcac cgccaggatc
      301 gtcaccagga cgagaccggt cagggcccag cggtcgcggg cgaaacgccg ccaatggcgt
      361 ggcgcttgca tgggggtctc gactacgtta ctcgggactc gcgggacaaa tcaagcgcag
      421 acacggatgg caaggcggtg aatcccggca cccggttcac cctcgtcctc gggggcggcg
      481 gcatgaaggg cctggcgcac gtcggcgtgc tcgaggccct gctggagcgc ggcctcaagc
      541 cctcccacat cgtcggctgc agcgtcggct ccctggtcgg ggccgcgtgg tcgtccggca
      601 agagcattcc cgagctgcgc gacatcgcca tcaacctgaa gcgcaaggac gtcttccaga
      661 tcgcccacgc cgacatggcg ttcaagcgca tgcgctcccc cgccctgttc cggcgcgagc
      721 cgctggcgct gctgatcgat cggctggtgg gcgacgccgc gttcgccgac ctggatgtcc
      781 ccgtcatctg caataccgtc gacatcaact cggggatgca ggtgctctgg ggactgcccg
      841 ggctggacga catccccgtg ggcgaagcgg tgttcggctc gtgcgcgctg ccgggctacc
      901 tcccgcccca cgagatccgg gggcgccact tcgtggacgg ggcggtgatc gagaacctcc
      961 cgatcgcggc cgcgcgcgcg ctcgacagcg agatggtctt cgcggtcgac gtgtcggcgt
     1021 cgagcacgct gcgcgaggac acgcaggagg ggggcttcgc cgccgtgttc ggacgcgcga
     1081 ccgagatcgc aatgcaggcg ctgctggagc agcggctacg gcagtggacc accccgcccg
     1141 tctactacat ccacccgcgc gtcgagcaca tctcgatgtt ctcgttcgat catctgcgcg
     1201 aagtcgtcga agaaggctac cgcgccacga cggaagcgct cgagcgcgcg gacacctggc
     1261 cggcgcgcgg cgctgagggc gtgtacccgc gccggcgtgt gatcgtccag gtcgcgcgcg
     1321 atcgctgcat cggatgcggc gcctgtctgt tccagggacc gccggggctc ttcgtcctcg
     1381 acaccgagcg caaagcggtc gtcacgcagc cggagcagga gtggacccct gccagcggca
     1441 gtttcatccg gcactgcccc acctacgcca tcagcgcgcg cccggtcccc gcggcgatgg
     1501 agacgatgcg acgctccggc tgagtggcac ggagatgcgc tcaccccgtc tggagtaacg
     1561 caccttactc gttgtcctca cagtgttttc cggcgtgctt cttgcccctg cctgggggcg
     1621 gcgcggaagc tcgtcggtgg tcgatcggct aactccgcga aacgacggac acatcgacgc
     1681 tggtcccacg cacggcggct cgacacgttc ggccgcggtg tggaggcccc gcaacaccag
     1741 gtagtcttcg cggaggacca tccggttgcg tatcaagttc cagcacgcgg cgattctcac
     1801 gccgtccgcg ccggcgcgcg ccaaccagaa accgggcgcc accgcgcttc ctccgtcccc
     1861 cggccagccc cccgccgtca tccccgcatc gcgaccgagc gtgcgaggca agttcctctt
     1921 cctcggagac gaaaaactcc acgtccgtgg ggtgacgtac ggcacgttcc ggccccaggc
     1981 cgacgacagc gagttcccga cggccgatgt cgtggagcgg gacttcgcgc tgatggccgc
     2041 acacggggtg aactcgctgc gcacctacac ggtcccgccc cgctggctgc tcgattcggc
     2101 ctcgcgccac ggcctgcacg tgctggtcgg gatcccggtg gagcggcaca tcgggttttt
     2161 gacggaccgc aagggcgcgc ccgacacggg tcgcctcgtg cgcagcggtg tcgcggcgtg
     2221 cgccggtcac ccggcggtgc tggcgtacgc gatcgggaac gagatccccg cggccacggc
     2281 ccggtggcac ggcgccggcc gcgtggaacg gttcctggag aaactgtttc gcgccgtcaa
     2341 ggccgaggat ccgtcgggcc tcgtgacgta cgtgaactat ccctccaccg agtacctgca
     2401 gctccccttt ctcgatttcg tctccttcaa cgtctttctc gagtcgcgcg ccccgctggc
     2461 cgcgtacctc gcgcgtctgc atcacgtcgc cggcgagcgg ccgctcctcc tcaccgaggt
     2521 cggcctggac agcctgcgca acggcgacgc cgcgcaggcg cgtacgctcg actggcagat
     2581 ccgcaccagc ttcgcggaag gctgcgccgg ggtcttcgtc tacgcctgga ccgacgagtg
     2641 gtaccgggcc ggtgaggacg tgaccgactg gcgcttcggt ctcacgagcc gtgaccggag
     2701 ccccaagccg gcgctcgccg ccgtgcaggc cgccttcgcc gacacgccgt tcccggccga
     2761 gatgcgctgg ccgcgcatgt ccgtcgtggt gtgcacctac aacggcagcc gcaccatccg
     2821 cgaatgcatg gaaggcatcc gtcggctccg ctatcccgac ttcgaagtga tcgtcgtgaa
     2881 cgacggctcg accgaccgga cggcggagat cgtgcgggag tacgacgtgc gcctgatcag
     2941 cacggacaac cgcggcctct cgagcgcgcg caacaccggg tggcgcgaag cgacgggcga
     3001 gatcgtcgcc tacatcgacg acgacgccta tccagacccg gactggctga cgcacctggc
     3061 ggccaccttc ctgcgcacca ccgacgtcgg cgtcggcgga ccgaacatcg cgccccccgg
     3121 cgacggcccg atcgcccggt gcgtcgccct ggccccggga aatccgatcc atgtgatgct
     3181 gaccgaccgc gaggcggagc acatccccgg ctgcaacatg gcgttccggc gggacgcgct
     3241 cgcggcgatc ggcggcttcg acgagcggtt ccgtatcgcc ggggacgacg tcgacgtgtg
     3301 ctggtcgttg cgggagcgcg gcctgacgct gggattcagt cccgccgccg tcgtgtggca
     3361 ccaccggcgc aattccgtgc gcgcattctg gaagcagcag gtcggttacg gacgggccga
     3421 aggcctgctg gagcggaagt ggccggagaa atacacggcc accggccaga tcgcctgggc
     3481 cggacgcctg tacggcgcca cgctattgcc cgccgcgccg tggcgcagcg gacgcatcta
     3541 ccacggcacc tggggcttcg cgccgtttca gcggctgtac caggcgacgc ccacgtggat
     3601 cggatcgctc gccctcgcgc ccgagtggta tctgccgatc gccgcgctcg cggtggtggc
     3661 gctgctcggc atcacctggg cgccgctgcg cctggccatc cccctgctgg cgctcgcgac
     3721 cgccccgctc atcatgcggg cggcgatcgc cgcgcgcata tcctttccac gctcccgtcg
     3781 cacgcggcgc atccggcaac ggctgctgac cggctacctg cacctgctcc atcccctggc
     3841 ccggctctac gggcgattga cgcgacccgc gacccaccgg gcgcgccgtg ggtgggcgtt
     3901 gggggccacg gtcgccctgt ggtgcgagcg ctggcgggat cccggcgagc gactcaacgc
     3961 catcgagcgg gcgctgcgcg agtcggaagc gggagcggtg ttgcgaggcg gtgatttcga
     4021 gcgctgggac ctggaagtcc gcggcggaac gctcggggcg gcacgtctcg cgatggccgt
     4081 cgaggaacac ggcatgggtc gccagctggt acgggtccgc tggtggcttg ccccttcccc
     4141 ggccggaccg gcgctggccc tgctgctcgg cggcctggcc atcgtcgcgg agaccaccag
     4201 cgtgtggatt ccgggtgccg tgttcggggc cgcggcgctc tggtgtgcct ggcggaccat
     4261 cgcggcgtgc ggctcggccg cagccgacat ccggcgggcg gtcggccgcg cgggggacgg
     4321 ctgaccgatg gcgggagcct acagcgactt caccctgtac cggcggctgc tcgcccaggc
     4381 gcgcccctac tggccgcatc tcgg
//
LOCUS       DBNGZW010000753         4080 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1422221, whole genome shotgun sequence.
ACCESSION   DBNGZW010000753 DBNGZW010000000
VERSION     DBNGZW010000753.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4080)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4080)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4080
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1422221"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="AC16JC_17600"
     CDS             complement(<1..246)
                     /locus_tag="AC16JC_17600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OPT/YSL family transporter"
                     /protein_id="HIR5166572.1"
                     /translation="MKPKELTIRGLILGALITTGFTAANVYLGLKVGLTFASSIPAAV
                     ISMAVLSAVKDSTSLENNIVQTVASAAGTLSAIIFVLP"
     gene            complement(243..2945)
                     /locus_tag="AC16JC_17605"
     CDS             complement(243..2945)
                     /locus_tag="AC16JC_17605"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase domain-containing protein"
                     /protein_id="HIR5166573.1"
                     /translation="MTDALTRLSAALADRYRIERELGQGGMATVYLAQDLKHDRRVAV
                     KVLRPELAAVIGAERFLSEIKTTANLQHPHILALFDSGRTGGQPDGRSDDFLYYVMPY
                     IEGETLRDKLNRDTQLGIDEAVRIATEVASALDYAHRHGVIHRDIKPENILLHDGRAL
                     VADFGIALAASKAGGTRMTETGMSLGTPTYMSPEQAMGEREITARSDVYALGCVTYEM
                     LLGEPPFTGPTAQAIVAKLLTEKPARLIPRRDRIPPEVEDAVLTALEKLPADRWGSAK
                     EFADALTGRVARRHAHPAAAATARGYRRVILPAALGIAIVATAFAVYFARQAGHRQAT
                     GMGVSRFQISLPNLRAGQLRYTGNPFVLSPDGRHLAYVGSSATGGPQLWVRDRDNLTP
                     RPLAGTDGADGPFFSPDGRWIGYSSQGRLYKVPTSGGAPIALADSVSVTVPGGAWLPT
                     GQIPFVGVGFGLMRVAASGGSAELLLPGTFNGNFFPTPLPRPDALLLTQCTNNCAQMT
                     LVAVNLKTHALDTLSANTARAWYLPTGNLVLARQDGSLYGARFDVATLKISGGVVPLL
                     SGVQLELGVVPEVSIADDGTLLYLPADPRGGDVRVVRVDRQGRPVALDPDWQANINTL
                     ALSADGRRLAVSIAAEGRTDLWVKQLDAGPLTRLTFSGTLNYRAAWEPGGRSLSYTSD
                     QDALSHLYRIRADGSGKPERLMPEDTAQIDESLWSPDGRWLVYRAGVVDGTRNIFARR
                     LGADTARLTIAARGFDEYMPTVSPDGRWIAYVSVESGHEEVYVRPFPDVERARWQVST
                     AGGAHPVWAHSGRELFYVTATDSLVAVDVAGTRDFATGARRGLFSTRSFLIQPYHQGL
                     AVTPDDRAFIMLQRAVATGSQAADLTVVLNWFTEVRTKTSARSP"
     gene            complement(2942..>4080)
                     /locus_tag="AC16JC_17610"
     CDS             complement(2942..>4080)
                     /locus_tag="AC16JC_17610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166574.1"
                     /translation="VQVSAELTDPRTGESLWARTFSRPAPDVVTLQHEVALEIARGIR
                     ARLTPEQERALGAAAPVNPKAYAQYLLGEEQANLRTPEGFRRSVEYLNRSIVLDSTFA
                     PAWATLAVTHGVAIFFQLISPDSARAAIGRASARALALDDRLGDPLIARGMMHYLLDW
                     DFATAGREFTSGMARNPTTLAQALYSWFPWGTGQFAEGIAVSSRLIDMEPTTAQWYSD
                     VGWNYWSSGEGAKARTFARRAIALDSAFYEPYHLLAWIEAQAGDFPTARWALARARAA
                     SGGFWMQELVEGFIRARSGDTAGARRVLRALDRDPRLAQRALLLLALGDRDSMYTLLA
                     RAIDARDPDALWMLNSAPLLRPLRHEPRYQRLLERMGLPENLRQ"
BASE COUNT          638 a         1390 c         1444 g          608 t
ORIGIN      
        1 ggggaggacg aagatgatcg ccgagagcgt gccggcggcg gaggcgacgg tctgcacgat
       61 gttgttctcg agactcgtcg aatccttgac cgcgctgagc acggccatgg agatcaccgc
      121 cgcagggatc gaggaggcga aggtgaggcc gactttgaga ccgagataga cgttggcggc
      181 ggtgaagccg gtggtgatca gcgctccgag gatgaggccc cggatggtga gttccttcgg
      241 tttcatgggc tcctcgcgga cgtcttcgtc cgcacctcgg tgaaccagtt gaggacgacg
      301 gtgagatccg cggcctgcga tccggtcgcc accgcccgct gcagcatgat gaacgcccgg
      361 tcgtcgggcg tgaccgccaa cccctggtgg tacggctgga tgagaaagct ccgcgtcgag
      421 aagaggccac gccgcgcacc ggtggcgaaa tcccgcgtgc ccgcgacatc cacggcgacc
      481 aggctgtcgg tggcggtcac gtagaacagc tcgcggccgc tgtgcgccca caccggatgc
      541 gcgcccccgg cggtcgagac ctgccagcgg gcccgctcga cgtcggggaa gggccgcacg
      601 tagacctcct cgtgtcccga ttcgaccgac acgtaggcga tccaccggcc atcgggtgag
      661 acggtgggca tgtactcgtc gaacccgcgc gccgcgatcg tcagccgcgc ggtgtcggcg
      721 cccaggcggc gggcaaagat gttccgcgtt ccatcgacga ccccggcccg gtagaccagc
      781 cagcggccgt cgggcgacca gagcgactcg tcgatctgcg ccgtgtcctc cggcatgagg
      841 cgctccggct tgccgctgcc gtcggcccgg atgcgataca gatgcgacag cgcgtcctgg
      901 tccgacgtat acgagaggga ccggccgccc ggctcccatg cggcccggta gttcagggtg
      961 ccgctgaaag tgagccgggt gaggggtccc gcatcgagct gcttgaccca caggtccgtg
     1021 cggccctcgg ccgcgatgct caccgcgagc cgccggccgt cggccgagag cgcgagcgtg
     1081 ttgatgttgg cctgccagtc gggatcgagt gccaccggcc gtccctgacg gtccacacgc
     1141 accacccgta catccccgcc ccgcggatca gcgggcagat acagcagcgt gccgtcatcg
     1201 gcgatcgaca cctcgggaac gacgcccagc tcgagctgca caccgctcag caacgggacg
     1261 acgccgccgc tgatcttcag cgtggccaca tcgaagcggg ccccgtatag cgacccgtcc
     1321 tgacgagcga gcaccaggtt gcccgtcggc agataccagg cgcgtgcggt gttcgcggac
     1381 aacgtatcca gggcatgggt cttgagattc accgcgacga gcgtcatctg ggcgcaattg
     1441 ttggtgcact gggtcagcag cagggcgtca gggcggggca acggggtcgg gaagaagttg
     1501 ccgttgaatg tgcccgggag gagcagctcg gcggatccgc cgctcgccgc aacgcgcatc
     1561 aggccgaagc ccacccccac gaagggaatc tgcccggtag ggagccaggc gcccccgggt
     1621 acggtgaccg acacggaatc ggccagggcg atcggggcgc cgccactcgt gggaaccttg
     1681 tacaggcgtc cctgcgagga gtaaccgatc catcgaccat ccggggagaa gaacggtccg
     1741 tcggcgccgt cggtcccggc gagcgggcgg ggggtgaggt tatcccggtc ccgtacccag
     1801 agctgggggc cgcccgtggc gctcgagccg acgtaggcga ggtgcctgcc gtcgggggac
     1861 agcacgaagg gattgccggt atagcgcagc tgcccggcgc gaagattggg gaggctgatc
     1921 tggaagcgtg acacccccat ccccgtcgcc tgccggtgac cggcctgccg ggcgaagtag
     1981 acggcgaacg cggtggcgac gatcgcgatt cccaacgcgg ccggcaggat gacgcgacgg
     2041 tagccccgcg cggtggccgc cgcagcggga tgggcgtgtc gacgtgccac cctccccgtc
     2101 agggcgtcgg cgaactcctt cgcgctcccc cagcgatcgg ccgggagctt ttccagcgcg
     2161 gtgagtacgg cgtcctcgac ctcgggcggg atgcggtcgc gtcgcgggat gagccgcgcc
     2221 ggtttctccg tgagcagctt cgccacgatg gcctgggcgg tggggccggt gaacggcggc
     2281 tcgcccagca gcatctcgta ggtcacgcag ccgagcgcat acacgtccga gcgcgcggtg
     2341 atctcccgct cccccatcgc ctgctcgggg ctcatgtagg tcggggtgcc gagcgacatc
     2401 ccggtctcgg tcatccgggt gccgccggcc ttgctggcgg cgagcgcgat gccgaaatcc
     2461 gcgaccagcg cccggccgtc gtgcagcagg atgttctcgg gcttgatgtc gcgatgaatg
     2521 accccatggc ggtgggcgta gtcgagggcg cttgccactt cggtcgcgat ccgcaccgct
     2581 tcgtcgatcc ccagctgcgt atcgcgattc agcttgtcgc gaagggtctc tccttcgata
     2641 taaggcatca catagtagag gaagtcatcc gaccgcccgt ccggctgacc gcctgtccgc
     2701 ccgctgtcga acagggcgag gatgtgcggg tgctggagat tggcggtggt cttgatctcc
     2761 gagagaaacc gctccgcgcc gatcaccgcc gccagctcgg gccgcagcac cttcaccgcg
     2821 accctccgat cgtgcttcag gtcctgggcg aggtacacgg tggccatgcc gccctgcccc
     2881 aactcgcgct cgatgcggta acggtcggcg agcgccgccg acagcctggt cagtgcgtcc
     2941 gtcattgccg cagattctcc gggaggccca tgcgctccag caaccgctga taccgcggct
     3001 cgtggcgcag cggacgcagc agcggcgccg agttgagcat ccagagagcg tcggggtccc
     3061 gcgcatcgat cgcgcgcgcg agcagcgtgt acatcgaatc gcggtcgccc agcgcaagga
     3121 gcagcagggc ccgctgagcc agccgcggat cgcggtccag cgcccgcaac acgcggcggg
     3181 cgccggctgt atcgcccgat cgggctcgga tgaacccctc gaccagctcc tgcatccaga
     3241 agcccccgct cgcggctcgg gcgcgtgcca gagcccaccg cgccgtcggg aagtctcccg
     3301 cctgagcctc gatccaggcg agcaggtggt acggctcgta gaacgcgctg tcgagcgcga
     3361 tcgcccggcg cgcgaacgtg cgcgccttcg ccccctcgcc actcgaccag tagttccagc
     3421 ccacgtcgct gtaccactgg gcggtcgtcg gttccatgtc gatgagccgg gaggagacgg
     3481 cgatgccctc ggcgaactgt ccggtgcccc acgggaacca actgtagaga gcttgcgcca
     3541 gcgtggtcgg attgcgtgcc atgccgctcg tgaattcccg cccggcggtc gcgaagtccc
     3601 agtcgaggag gtagtgcatc atgccgcgtg cgatcagcgg gtcccccaac cggtcgtcca
     3661 gggcgagcgc ccgcgccgac gcccgcccga tcgccgccct ggctgagtcg ggagagatga
     3721 gctggaagaa gatcgcgacg ccgtgggtga cggcgagtgt cgcccacgcg ggtgcgaaag
     3781 tcgagtcgag cacaatggaa cggttcagat actcgacgct gcgacggaac ccttcgggag
     3841 tgcggaggtt cgcctgctct tcgccaagaa gatactgcgc gtacgctttc gggtttaccg
     3901 gcgccgccgc gccgagcgcc cgttcctgtt ccggtgtgag ccgggcccgg atcccccggg
     3961 cgatctcgag cgcgacctcg tgctgcagcg tcaccacgtc gggcgccggc cgggagaagg
     4021 tccgggccca gaggctctcc ccggtgcgcg ggtcggtcag ctccgccgac acctgcacga
//
LOCUS       DBNGZW010000754         2609 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_742258, whole genome shotgun sequence.
ACCESSION   DBNGZW010000754 DBNGZW010000000
VERSION     DBNGZW010000754.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2609)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_742258"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..221
                     /locus_tag="AC16JC_17615"
     CDS             <1..221
                     /locus_tag="AC16JC_17615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR5166575.1"
                     /translation="PDGKWIAYLAFPPDVAADDHPFYKHVLLRLMPVGGGPARVIAYV
                     YGGQGTINVPSWSPDGTRLAFVSNSVMP"
     gene            242..>2609
                     /locus_tag="AC16JC_17620"
     CDS             242..>2609
                     /locus_tag="AC16JC_17620"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012906.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 protein"
                     /protein_id="HIR5166576.1"
                     /translation="MRRISRVLGLLLLAAGVMRAQERLALHAGWLLRSSAGIGVDGAA
                     LSSPTYRPTGWYRATVPSTVAGALVEDGVYADPFFAMNLRALPGMTYAIGANFVHTPM
                     DPRSPFAVPWWYRTTFRVPATMRDRRVVLHFDGINYRANIWLNGRRLADSSMVAGTYR
                     RYEFDVTDAVRSTGLNGLAVEVFAPTPLDLQTTWVDWNPSPPDKNMGLWQPAYLTASG
                     DVVVRYPEVVSRVDTATLRSAELTVVTGLRNLAAHPVTGTLRGRIGAVAFSRPVTLAP
                     HDSALVRFTPDSFPQLRFANPRLWWPAELGMTEPQLYDLALEFVTGGRVSDSQGVRFG
                     IREITSASTPQGGRLFRVNGRRILIRGGGWAPDLFFRPQPERQDAQLRYALDMHLNTI
                     RLEGNYENDRFWHQTDSLGLLVMTGWVCCSAWEEWAKWGPEQHAVAAASLRDQIRRLR
                     DHPSALVWLNGSDGPPPADVERMYLAIEQQEAWPNPTISSASAEPTEVSGASGVKMTG
                     PYDWVPPSYWLQDSTHGGAWAYNTETSPGAAVPPIESMRRMIPARDIKWPLDSVWLFH
                     AAGGQFTHLLDRFNTALATRYGPPPSVEDYTVTAQLMTYEGERAMFEAYRRNAYVSTG
                     VIQWMFNNAWPSIYWHLFDWYLLPAGGYFGAKKANEPVHVLFSYDDRSVVVVNGGAAR
                     TPLRAARLRARILGLDGSEQFVHDTVLDLPPDSSARVFVLPEPTGVPGGAAYFADLRL
                     TSADGRPLSTNLYWLSTRPDVLADTSTWYMTPVKSFADFTALRSMPAAA"
BASE COUNT          394 a          904 c          894 g          417 t
ORIGIN      
        1 cgccggacgg caagtggatc gcgtacctcg cgtttccacc cgatgttgcc gcggacgatc
       61 acccgttcta caagcacgtg ctgctgcgac tgatgcccgt gggtggcgga ccggcgcgcg
      121 tgatcgccta tgtgtacggc gggcagggca cgatcaacgt cccgtcgtgg tcgcccgatg
      181 gcacgcgact cgcgttcgtg agcaattccg tgatgccata aggcttcgag gaggcaacat
      241 cgtgaggcgg atctcgaggg tcctcggtct tctactcctg gctgcaggcg tcatgcgggc
      301 acaggagcgg cttgcgctgc acgccggctg gctcctcagg tcgtcggcgg gtatcggcgt
      361 ggacggcgcg gcgctgtcgt cgcccaccta ccgtccgacg gggtggtacc gcgcgaccgt
      421 gccgtcgacg gtggcgggcg ccctggtcga ggacggggtg tatgccgacc cgttcttcgc
      481 catgaacctc cgcgcgctcc ccgggatgac atacgcgatc ggcgcgaact tcgtgcacac
      541 gccgatggac ccgcggagcc ccttcgccgt gccctggtgg tatcgcacga ccttccgcgt
      601 tcccgcgacg atgcgcgacc ggcgcgtcgt gctgcacttc gacgggatca actatcgcgc
      661 gaatatctgg ctgaacggcc gccggctcgc ggattcgagc atggtggccg gcacgtaccg
      721 gcggtacgag ttcgacgtca ccgacgcggt gcgatcgacc ggactgaacg gactcgccgt
      781 cgaggtcttt gcgcccaccc cgctggacct gcagacgaca tgggtcgact ggaacccgtc
      841 gccaccggac aagaacatgg ggctgtggca gccggcctac ctcaccgcga gcggcgatgt
      901 cgtcgtccgc tacccggagg tcgtgagtcg cgtcgacaca gccacgctgc ggagcgcgga
      961 gctgaccgtc gtgacgggtc tcaggaatct cgccgcgcac ccggtcacgg ggacgctgcg
     1021 cggccggatc ggagccgtcg ccttcagccg gcccgtcacg cttgcgcccc acgacagcgc
     1081 gctcgtgcgc ttcacgccgg acagcttccc acagctgcgc ttcgcgaacc cgcgtctctg
     1141 gtggcccgcg gagctgggga tgaccgagcc ccagctgtac gacctggcgc tcgagttcgt
     1201 gaccggcggg cgcgtgtcgg acagtcaagg cgtgcgcttc ggcattcgcg agatcacgtc
     1261 ggcatcgacg ccgcagggcg ggcggctctt ccgcgtgaac ggccgccgca tcctgattcg
     1321 cggcgggggc tgggcacccg atctgttctt ccgcccgcag cccgagcggc aggacgcgca
     1381 actccgctac gcgctggaca tgcacctcaa caccattcgg ctcgaaggca attacgagaa
     1441 cgatcgcttc tggcaccaga ccgacagcct cggcctgctc gtgatgaccg ggtgggtgtg
     1501 ctgcagcgcc tgggaggaat gggccaagtg ggggccggag cagcacgccg tcgcggcggc
     1561 ctcgctgcgc gatcagatcc gccggctccg cgatcatccc agcgccctcg tctggctcaa
     1621 cggcagcgac ggtccgccgc cggcggacgt cgagcggatg tatctcgcga tcgagcagca
     1681 ggaggcgtgg cccaacccta cgatctcctc ggccagcgcc gagcccaccg aggtcagcgg
     1741 ggccagcggc gtcaagatga ccggtccgta cgattgggtc ccgccgtcgt actggctgca
     1801 ggacagcacg cacggcgggg cctgggcgta caacaccgag acgagccccg gggccgcggt
     1861 gcctccgatc gagagcatgc gccgcatgat tcccgcgcgc gacatcaagt ggccgctgga
     1921 cagtgtatgg ctcttccacg cggcgggcgg ccagttcacg cacctgctag accggttcaa
     1981 caccgcgctg gcgacgcgct acggcccgcc gccgagcgtc gaggactaca cagtcaccgc
     2041 gcagctcatg acgtacgagg gtgagcgggc gatgttcgag gcgtaccggc ggaatgcgta
     2101 cgtctcgacc ggcgtgattc agtggatgtt caacaacgcc tggccgtcca tctactggca
     2161 cctgttcgac tggtacctgc tgccggcggg cggctacttc ggagccaaga aggccaacga
     2221 gccggtgcac gtgctgtttt cctacgacga tcgatcggtg gtcgtcgtga acggcggggc
     2281 cgcccgcacg ccgctgcgcg ccgcgcgcct gcgggcccga atcctgggtc tcgacggctc
     2341 ggagcagttc gtgcacgaca ccgtgctcga cctgcctccc gacagcagtg cgcgcgtgtt
     2401 cgtgctgccg gagccgacgg gtgtgccggg cggagcggcg tatttcgcgg acctccgact
     2461 gacgagcgcc gacggacgcc ccctcagcac caacctgtac tggctctcca cccgtcccga
     2521 cgtgttggcc gatacctcga catggtacat gacgccggtg aagtcgttcg ccgacttcac
     2581 ggcgctcaga agcatgcccg cggccgcgg
//
LOCUS       DBNGZW010000755         4936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_467944, whole genome shotgun sequence.
ACCESSION   DBNGZW010000755 DBNGZW010000000
VERSION     DBNGZW010000755.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_467944"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1889)
                     /locus_tag="AC16JC_17625"
     CDS             complement(<1..1889)
                     /locus_tag="AC16JC_17625"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015343 - siderophore-iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0038023 - signaling receptor activity
                     [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor family protein"
                     /protein_id="HIR5166577.1"
                     /translation="MGWLIGAAAHAQQPDTTSTNALGLPAVSVSATRSTLPLAKLPFS
                     VQIVDRRQISAARPTWGLDEALTSVPGVYVGNRYNFSQDQRLSIRGFGSRSAFAVRGI
                     KVLLDGIPQTLPDGQGQLTNVELGTVDRIEVLRGSASALFGNAAGGVISLWTDPVPPQ
                     RATEHVRFVAGRFGRRSDRTWTKWQSTTSVRLGAGLAQVTASRLAYEGERDHSAADLR
                     NYNARVRFPVGSAWSLSGLLDVGDDPRADNPGSLTLAELLVNRDGVPALNLNRHAGKD
                     VTQFQAGGTLRRTSADGGEVTFTVFGLTRDLRNPITTAYIDVGRTDYGVRATFTRPLL
                     LGSLQSHLTAGFDFQHQRDDRRNWSYLNTPGDSATRSNTVTLDQLERVTEIGPFVQSA
                     LELTPRTTLTAGVRYDWVRFDVRDHLISSTNPDDSGERLMRSLSGSLGIALNPSDAMT
                     LYANVGSSFETPTTTELANRPDTAGGFNSGLEPQRALSYEIGARGRFGARVAYSVALF
                     QADVRGELIPYEIAAPRFFYRNAGSSRHRGVELGTDLSLVAGVALNATWTYADYRFRH
                     YRFTTAGQAFTLDGREMPGVPKHWLHLTLRVRPPATRGAWAEVEQTYSSGYLVGDTVS
                     TSAASS"
     gene            complement(1995..2693)
                     /gene="lepB"
                     /locus_tag="AC16JC_17630"
     CDS             complement(1995..2693)
                     /gene="lepB"
                     /locus_tag="AC16JC_17630"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR5166578.1"
                     /translation="MRDKLVAEARSWGKAIVIAVAIWLVLRSYVVEAFRIPSGSMENT
                     LLVGDFLFVDKTGYGGEFQIPFTGVRFGRLPGYATPQRNGLVIFRSVEDSTPNLDIVK
                     RLMGVPGDTLQMVNDTVIRNGQRLDEPYALHLGDPGVSSSQLEEMRALQLPHYVGTDP
                     GHYRPTLHTWGPVVVPPDSYWVMGDNRDESRDSRFWGFLPRSHIEGRPLFIYMSIALQ
                     PFRIRWNRLLRHPG"
     gene            complement(2702..3715)
                     /locus_tag="AC16JC_17635"
     CDS             complement(2702..3715)
                     /locus_tag="AC16JC_17635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006927508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166579.1"
                     /translation="MLPLLLVTAVLQQAAVAPDSGETHLRNIRQITFGGQNAEAYFSH
                     SGRQLILQRTPGDSGCDQQYVVNTDGTGLHRVSGGLGRTTCGYFFAGDARIFYASTEH
                     ADTACPPRPDYSQGYVWALYDYDIYVADSSGGHKQRLTRNRGYDAEGTLSPDGRTIVF
                     TSLRNGDLDIYTMRTNGTHLRRLTHALGYDGGPFFSPDGKQIVYRASRLTTARDSADY
                     RALLAKNLVRPTQMDLWVMNADGSGQRRVTQLAGASFAPYFTPDGKRIIFATNFQNPR
                     SRNFDLYLVNPDGTGLEQVTTNPDFDAFPMFSPDGQRLVWASNRHGSVPGETNIFIAD
                     WVP"
     gene            3817..4134
                     /locus_tag="AC16JC_17640"
     CDS             3817..4134
                     /locus_tag="AC16JC_17640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166580.1"
                     /translation="MGDPQSSRRQPPLLDALGKLCVEGKQAADYLWQVPDDAAVRQRI
                     LGMLDKIEVESRAQGRREMPQVALELKVAARASPSPQQVDLLVNGFDRLYHLWEAAKS
                     GLF"
     gene            4185..>4936
                     /locus_tag="AC16JC_17645"
     CDS             4185..>4936
                     /locus_tag="AC16JC_17645"
                     /EC_number="2.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011997950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016744 - transketolase or transaldolase
                     activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HIR5166581.1"
                     /translation="MDAVQQADSGHPGTAMALAPLAWVLWQRHLRVDPADPAWPDRDR
                     FVLSCGHACMLVYAALYLSGFDLSLDDLKQFRQWGSRTPGHAEHVTPGVEVTTGPLGQ
                     GVGNAVGLAIAEAQLAALFNRPGHTVVDHYTYFLASDGDLMEGISHEACSLAGHLRLG
                     KLIGFYDDNRITIDGATDLTFSDDTARRFEAYGWHVQHLPDGNDLDAIDHAIRHAHAA
                     ADRPSLIILRTHIAYGSPNKQDTAEAHGAPLGA"
BASE COUNT          792 a         1704 c         1659 g          781 t
ORIGIN      
        1 gacgacgcgg cactcgtact gacggtgtcc ccgaccagat atccggacga gtaggtctgt
       61 tcgacctcgg cccaggcgcc gcgggtcgcc ggcggccgga cgcgcagcgt caggtgcagc
      121 cagtgcttcg gcaccccagg catctcccgc ccgtcgagcg tgaacgcctg gcccgccgtc
      181 gtgaagcggt agtgccggaa ccggtagtcc gcgtaagtcc acgtggcatt cagggccacc
      241 cctgcgacca gggagaggtc cgtcccgagc tccacgccgc gatggcgcga gctcccggca
      301 ttccggtaga agaaccgcgg tgcggcgatc tcgtagggta tcagctcccc gcgcacgtct
      361 gcctggaaca acgccacgga gtacgccacc cgcgcgccga accggccgcg cgcgccgatc
      421 tcgtagctca gcgcccgctg cggttcgagc ccgctgttga acccgccggc ggtatcgggc
      481 cggttcgcca gctcggtcgt cgtgggcgtc tcgaaggatg agcccacgtt ggcgtacagg
      541 gtcatcgcgt ccgacgggtt cagagcgatg cccagggagc ccgacaacga ccgcatcagg
      601 cgctcgccgc tgtcgtccgg attggtgctc gagatgaggt ggtcccgcac gtcgaacctc
      661 acccagtcgt atcgcacgcc cgcggtcagc gtcgttcgag gcgtcagctc cagcgcactc
      721 tgcacgaatg ggccgatttc ggtcacccgc tccagctgat cgagcgtcac cgtgttgctg
      781 cgcgtggcgg aatcacccgg cgtgttcaag tagctccagt tgcgccggtc gtcgcgctgg
      841 tgctggaagt cgaatcccgc cgtgagatgg gattgcagcg atcccagcaa cagcggccgg
      901 gtgaaggtcg cgcggacgcc gtagtcggtg cgaccgacgt cgatgtaggc cgtcgtgatc
      961 ggattcctca ggtcacgcgt gagcccgaac acggtgaagg tcacctcccc cccgtccgcc
     1021 gaggtccggc gcagcgtccc gcccgcctgg aactgcgtca catccttgcc ggcatggcga
     1081 ttgaggttga gtgccggcac gccgtcccga ttgacgagca gctcggccag ggtcagcgat
     1141 cccggattgt cggcgcgggg gtcgtcgccc acgtccagca gccccgacag cgaccacgcg
     1201 ctcccgaccg gaaatcggac ccgcgcgttg taattgcgca ggtccgccgc gctgtggtcg
     1261 cgctctccct cgtaggcgag ccgcgaggcc gtcacctggg ccagtccggc cccgagccgc
     1321 acgctggtcg tcgattgcca cttggtccag gtacggtcgc tgcgccggcc gaatcgcccc
     1381 gccacgaacc ggacgtgttc ggtcgcgcgc tgcggcggca ccggatccgt ccacagactg
     1441 atcacgccgc cggcggcgtt tccgaacagc gccgatgcgg agccgcggag gacctcgatc
     1501 cggtccacgg ttccgagctc gacgttggtc agctgaccct gtccgtcggg cagcgtctgg
     1561 ggaatgccgt ccaacaggac cttgatgccc cggacggcga acgcggagcg gctgccgaaa
     1621 ccccgaatcg ataggcgctg atcctgcgag aagttgtagc ggttgccgac gtacacgccg
     1681 ggcacgctcg tcagcgcctc gtcgagaccc caggtgggcc gggcggcgct gatctggcgg
     1741 cggtcgacga tctggaccga gaaggggagt ttggcgagcg gcagcgtcga gcgggtggcg
     1801 gacaccgaga cggcgggaag tccgagggcg ttcgtggaag tcgtgtcggg ttgttgagcg
     1861 tgcgccgccg cccctatcag ccaccccacc ccgtacgcca aaccgcgcca cacccgtgac
     1921 ctcgtcatgg ccccaggctt accaacgtca cgccgggacg tcaagcgcgg cgcccgctcc
     1981 gcagcgcggt ggacttaccc ggggtggcgc aacaggcggt tccagcggat acggaacggt
     2041 tgcagcgcga tgctcatgta gatgaacagc ggccgcccct cgatgtgcga gcggggcaga
     2101 aatccccaga accggctgtc ccgggactcg tcgcggttgt ctcccatcac ccaatagctg
     2161 tcgggcggga cgaccaccgg cccccacgta tgcagcgtcg gccggtagtg cccgggatcg
     2221 gtgcccacgt agtgcggcag ctggagggcg cgcatctctt cgagctggga gctcgacact
     2281 cccggatcgc ccaggtgcag ggcgtagggc tcgtccaggc gctgtccatt tcggatcacg
     2341 gtgtcgttca ccatctgcag cgtgtcgccg ggcacgccca tgaggcgctt cacgatgtcg
     2401 agattgggcg tcgagtcctc gacggagcgg aagatcacca gcccgttgcg ctgcggcgtc
     2461 gcatagccgg ggaggcggcc gaaccggact ccggtgaacg ggatctggaa ctcgccgccg
     2521 tagccggtct tgtccacgaa caggaagtcc ccgaccagca gcgtattctc catgctgccc
     2581 gacggaatgc ggaatgcttc caccacgtag gaccgcagga ccagccagat cgcgacggcg
     2641 atcacgatcg ccttccccca gctgcgtgct tcggcgacca gtttgtctcg catccccacg
     2701 actatggaac ccagtcggcg atgaagatgt tcgtctctcc cgggaccgag ccgtgccggt
     2761 ttgacgccca gacgaggcgc tgcccgtcgg gggagaacat cgggaacgcg tcgaagtccg
     2821 ggttcgtggt cacctgctcg agaccggtgc cgtccgggtt gacgagatag aggtcgaagt
     2881 tgcgggaccg gggattctgg aaattcgtcg cgaagatgat ccgcttgccg tccggcgtga
     2941 agtacggcgc gaagcttgcg cccgccagtt gcgtgacccg ccgctgcccg gagccgtcgg
     3001 cgttcatcac ccagaggtcc atctgcgtcg gccggacgag gttcttcgcg agcagcgcgc
     3061 ggtagtcggc gctgtcgcgg gcggtcgtca ggcgagacgc gcggtacacg atctgtttac
     3121 cgtcgggaga gaagaacggc ccgccgtcgt accccagcgc atgtgtcagc cgccggaggt
     3181 gcgtcccgtt ggtgcgcatc gtatagatgt ccaggtcgcc gttgcgcagc gacgtgaaca
     3241 cgatcgtccg gccgtccggg gagagggtcc cctcggcgtc gtagccgcga ttgcgtgtga
     3301 gacgctgctt gtgcccgccg ctcgaatccg cgacgtagat gtcgtagtcg tacagcgccc
     3361 acacataccc ctgcgagtag tcggggcggg gcgggcacgc tgtgtcggca tgctcggtgc
     3421 tggcgtagaa gatccgggcg tcgcccgcga agaagtagcc gcaggtcgtg cgccccagcc
     3481 cgcccgacac gcggtgcaga ccggtcccgt cggtattcac cacgtactgc tgatcgcatc
     3541 ccgagtcgcc gggagtacgc tgcaggatga gctggcgtcc cgaatgggag aagtacgcct
     3601 cggcattctg accgccgaag gtgatctggc ggatgttgcg caggtgcgtt tccccggagt
     3661 cgggagcgac ggccgcctgc tgcagcacgg cggtgaccag aagcagtggg agcataggtc
     3721 tcgcaagcta gaaggacgtg gaagctccgg caatccgaac gcccacgaac gtcggtggcg
     3781 ccttataccc cggtccggat tagattacgg cctcgtatgg gtgaccccca gtcatcgcgc
     3841 cgccagccac cactcctcga tgccctcggg aaactgtgcg tcgagggcaa acaggccgcc
     3901 gactacctct ggcaggtccc cgacgacgcc gccgtgcgcc agcgcatcct cgggatgctc
     3961 gacaagatcg aagtggagag ccgggcgcag gggcggcgcg agatgccgca ggtggcgctc
     4021 gagctgaaag tcgccgcccg cgccagcccc agcccccagc aagtcgattt gctcgtcaac
     4081 ggattcgacc gcctctacca cctgtgggaa gccgcgaagt cgggcctgtt ctgagcacga
     4141 cgtccgccga gcagctctgc atcaatgccg tccgcgtcct cgccatggac gcggtgcaac
     4201 aggctgattc cggccacccg ggcaccgcca tggcgctggc gccgctcgcc tgggtgttgt
     4261 ggcagcggca cctgcgggtc gaccccgcgg acccggcgtg gcccgatcgc gatcgcttcg
     4321 tcttgtcctg cggccacgcg tgcatgctgg tatacgccgc gctctacctc tcgggattcg
     4381 acctctcgct cgacgacctg aagcagttcc gtcaatgggg cagccgcacg cccggccacg
     4441 cggagcacgt gaccccgggc gtcgaagtca ccaccgggcc gctcggtcag ggcgtcggca
     4501 acgccgtcgg gttggcgatc gcggaggcgc agctcgccgc cctgttcaac cggcccggcc
     4561 acaccgtcgt cgatcactac acctacttcc tcgcctcgga cggcgatctc atggaaggga
     4621 tcagccacga ggcgtgttcg ctcgccggac acctgcggct gggcaaactg atcggcttct
     4681 acgacgacaa ccgcatcacc atcgacggcg ccaccgacct cacgttctcc gacgatacgg
     4741 cccggcggtt cgaggcgtac ggctggcacg tgcagcacct gccggacggc aacgacctgg
     4801 acgccatcga ccacgccatt cgccacgccc acgccgccgc cgatcgcccc tcgttgatca
     4861 tcctgcgtac ccacatcgcc tacggcagcc ccaacaagca ggacaccgcc gaagcgcacg
     4921 gcgctccgtt gggcgc
//
LOCUS       DBNGZW010000756         5989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1422934, whole genome shotgun sequence.
ACCESSION   DBNGZW010000756 DBNGZW010000000
VERSION     DBNGZW010000756.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5989
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1422934"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1279)
                     /locus_tag="AC16JC_17650"
     CDS             complement(<1..1279)
                     /locus_tag="AC16JC_17650"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013787079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HIR5166582.1"
                     /translation="MTTAVKVPTDIEIAQAAKLRPIADVAAELGLAEDEVEFYGRYKA
                     KISLQAIERRTPRGRLVLVTGINPTPAGEGKSTVTVGLAQALGRLGKRGILCIREPSL
                     GPVFGVKGGAAGGGYAQVVPMEDINLHFTGDFHAIASAHNLLSAMLDAHLHHGNALGL
                     DTRRITWPRTIDMNDRALRQIVVGLGGMNGGPTREERFVIIPGSEVMAIMALATSLAD
                     LEARLARIVVGLTPTKTAVRAADLKAQGAMTLLLKEALKPNLVQTLEGGPALVHCGPF
                     GNIAHGCNSVMATQLALRLADVVLTEAGFGADLGAEKFFDIKCRMAGLKPEAAVVVAT
                     VRALKMHGGKKKDQLATPDVAAVQRGVENLTRHVRNVKKFGVPCVVALNRFITDTDDE
                     IEAVRSACAAEAVDAVRCEVWEKGGEGGKELAQA"
     gene            complement(1276..2076)
                     /locus_tag="AC16JC_17655"
     CDS             complement(1276..2076)
                     /locus_tag="AC16JC_17655"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR5166583.1"
                     /translation="MLVDAHCHLADGAFDVDRAAVLARARRAGVGHVVVIGESVVGSE
                     RALALARATRGLSATAGVHPHEARTWSPEVERRLRELLADAAAVAMGETGLDYHYDHS
                     PRDAQRTAFESQLSLAAELGRPVVVPARAADDDIAALLRAWGRRVPAVVLHSFSGGDG
                     LFAAGMEIGAYFSFSGMITFKNWMHHDALTACPPDRLLVETDAPFLAPVPHRGRRNEP
                     AFVHDTAAELARRRGEPFEELACRTSENARRVFGDRIATTLDEREEPA"
     gene            complement(2079..3110)
                     /gene="secF"
                     /locus_tag="AC16JC_17660"
     CDS             complement(2079..3110)
                     /gene="secF"
                     /locus_tag="AC16JC_17660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR5166584.1"
                     /translation="MIRFFANASYDFIKWRRWAYALTATVIVPGLILLAVKGLNYSIE
                     FTGGTLIQIETARPVPTSELRADLDAAGIPGTEIQSFGSDTSYIIRARLGAQATTPGG
                     GTEETTAAVQRVLDTKLGVGSYRVERHEAVGPKVGRELQGKAFLAILFSFVVTLIYLA
                     IRFEWRFGLAAVLATLHDILTTIAFIRYFNLEVSLVVVGAVLTMVGYSLNDTIIIFDR
                     VRENLRKFRRQNLYEILNLSINETLPRSILTHGTTMAATLALVVFGGEVIRPFAMVMT
                     FAIFTGTFSSIYIAAPLLLWIEKRWPGQDVRGARAFAGRAPVAATPVAPAPVSPPPPG
                     SGGRSRAAV"
     gene            complement(3110..4738)
                     /gene="secD"
                     /locus_tag="AC16JC_17665"
     CDS             complement(3110..4738)
                     /gene="secD"
                     /locus_tag="AC16JC_17665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR5166585.1"
                     /translation="MSGIRGRIITIVGLLLISVWFLLPKDAKIRVPDKQTGRMKDTTI
                     RRVPINLGLDLQGGIHLALEVDQSKGPVADCADAIRRAERVVRTRVDEFGTTEPVVQV
                     VGNCRLVVELAGEKDPARAKQIVMQTAFLEFRITDMKNLFRDALPAIDNVLRQAGVKG
                     PTGAQVSAVSQLFGADTTKKGAAKDTTPDANAPGALSALLFQGQMPGEFLVPEEQVPR
                     AESLLARPEVQAAVPRGLDLKWGTATEVRSARSYRALYAVENRPIITGEELTKASARR
                     DQLNNHSVVVFDLSRRGSRKFCEETGRHVHDYMAIMLDNRVQGQPPIINDQICGTGQI
                     DLQNKPLTEAQDLALVLRAGALPAPLQIIEERTIGPSLGSDSIHDGIRAGIVGVVLVI
                     LIMVVYYRLSGVLAVSALGLYVVLTLAGLSVFGFTLTLPGLAGLVLSVGIAVDANVLI
                     FERIREELQHGKTVRLAVDEGFKHAMSAIIDSNVSTALTAFILYLVGTGPVQGFAITL
                     IIGIAASMVTAIFVVRTFYLVWLRQRPDMQTLSV"
     gene            complement(4735..5667)
                     /locus_tag="AC16JC_17670"
     CDS             complement(4735..5667)
                     /locus_tag="AC16JC_17670"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR5166586.1"
                     /translation="MSAVVTVGTFDGVHRGHQAVLAEIVARARRRGAESVVVTFDPHP
                     LAVVNPAAAPRLLTLPAERQRLLMEHGVDRVVTLPFTPQLSRLGPEEFVRDVLRLQVG
                     MDELVLGHDHAFGRGRAGDVEVVRRIGAADGFLVDVVDAVREDGQPISSTLIRAAVAH
                     GDLALAARWLGRPYAALGEVTRGAGRGRTIGVPTINLPVPDGRKLLPPDGVYAVWVTV
                     EGRGTGAEGRVERGRHGGMMNQGSRPTFGVAERGLEVHLFDFTDDLYGETVWVEWVRR
                     LRDVQTFASRDALVEQLARDAAAARETLERVSVR"
     gene            complement(5664..>5989)
                     /gene="truB"
                     /locus_tag="AC16JC_17675"
     CDS             complement(5664..>5989)
                     /gene="truB"
                     /locus_tag="AC16JC_17675"
                     /inference="COORDINATES: protein motif:HMM:NF020706.7"
                     /note="catalyzes isomerization of specific uridines in RNA
                     to pseudouridine; responsible for residues in T loops of
                     many tRNAs; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR5166587.1"
                     /translation="ARDVGEALGCGAHLVTLTRTAVGPFRLEDARAAEAVTAADLRPA
                     VSLVASLPSRELDEAERVAVVHGRAIPARAQDGPQVALLHAGRLVAVAEAEGDVLKPR
                     VVVTE"
BASE COUNT          928 a         2184 c         1995 g          882 t
ORIGIN      
        1 ccgcctgcgc cagctccttg ccgccttccc cacccttctc ccagacctcg cagcggaccg
       61 catcgacggc ctccgccgcg cacgccgacc gcacggcctc gatctcgtcg tcggtgtcag
      121 tgatgaagcg gttcagcgcc accacgcagg ggacgccgaa cttcttcacg ttgcgcacgt
      181 gccgggtgag attctcgacg ccgcgctgga cggccgcgac gtcgggcgtg gcgagctgat
      241 ccttcttctt gccgccgtgc atcttgaggg cgcgcacggt cgcgacgacc actgcggctt
      301 cgggcttgag gcccgccatg cggcacttga tgtcgaagaa cttttccgcc ccgagatcgg
      361 ccccgaagcc ggcttcggtc agcaccacat ccgcgagccg caaggcgagc tgtgtcgcca
      421 tgacggagtt gcagccatgg gcgatgttgc cgaacggacc gcaatgcacc agggcgggac
      481 cgccttcgag ggtctgcacc aggttgggct tcagggcttc cttgagcagc agcgtcatcg
      541 cgccttgcgc cttcaggtcc gcggcgcgca cggcggtctt ggtcggggtg agcccgacga
      601 cgatgcgggc gagccgcgct tccaggtccg cgaggctcgt ggccagggcc atgatggcca
      661 tcacttcgga gcccgggatg atcacgaacc gctcctcccg ggtggggccg ccgttcatgc
      721 cccccaggcc gacgacgatc tgccgcaggg cgcggtcatt catgtcgata gtgcgcggcc
      781 aggtgatgcg acgcgtgtcc agccccagcg cattgccgtg gtggaggtgc gcgtcgagca
      841 tcgccgagag gaggttgtgc gcgctcgcga tggcgtggaa gtcgcccgtg aagtggaggt
      901 tgatgtcctc catgggcacg acctgcgcgt agccgccccc ggcggcaccc cctttcacgc
      961 cgaagacggg gccgagcgag ggctcccgga tgcacaggat gccgcgtttc ccgagtctgc
     1021 ccagcgcctg cgccagaccc acggtgacgg tggacttccc ttctcctgcc ggcgtggggt
     1081 tgatccccgt cacgagcacc agccgacccc tcggcgtgcg gcgctcgatc gcctggagcg
     1141 agatcttcgc cttgtagcga ccgtagaact cgacctcgtc ctcggcgagg cccagctcgg
     1201 ccgcgacgtc ggcgatgggc cgcagcttgg cggcctgcgc gatctcgatg tcggtgggga
     1261 ccttcacggc ggtggtcacg cgggctcctc cctctcgtca agcgtggtag cgatgcgatc
     1321 gccgaatacg cgccgcgcgt tctccgacgt gcggcaggcc agctcttcga acggctcccc
     1381 gcgccgccgc gccagctcgg cggccgtgtc gtgcacgaag gcgggctcgt tgcggcgtcc
     1441 tcgatgcggc accggcgcga ggaacggcgc gtcggtctcc acgagcaggc ggtcgggcgg
     1501 acaggcggtc agtgcgtcgt ggtgcatcca gttcttgaac gtgatcatgc cgctgaacga
     1561 aaaatacgcc ccgatctcca tccccgccgc gaacagcccg tccccgccgc tgaacgaatg
     1621 caacacgacg gcgggcacgc ggcggcccca ggcacgcagc aacgccgcga tatcgtcgtc
     1681 cgccgcgcgg gcagggacga cgaccggacg ccccagttcg gcggccaggg acagctgtga
     1741 ctcgaaggcg gtgcgctgcg cgtcacgggg ggagtgatcg tagtggtagt cgaggccggt
     1801 ttcacccatg gcgaccgcgg ccgcatcggc caacagctcc cgcaggcggc gctcaacctc
     1861 cgggctccag gtgcgtgcct cgtgcgggtg gacgccggcc gtggcggaga ggccccgcgt
     1921 cgcccgggcg agggccaggg cgcgctcgct gcccaccacc gactccccga tcaccaccac
     1981 gtggcccacg ccggcccggc gggcgcgggc cagcacggcc gcgcgatcga cgtcgaacgc
     2041 cccatccgcc agatggcagt gggcgtcgac cagcacgctc agaccgcggc cctggagcgg
     2101 cctccgctac ccggaggggg cggtgaaacg ggcgccggag caacgggggt ggccgccacg
     2161 ggcgcccggc cggcgaaggc gcgggccccg cgcacgtcct ggcccggcca gcgcttctcg
     2221 atccacagca ggagcggcgc ggcgatgtag atggaggaga aggtgccggt gaagatcgcg
     2281 aaggtcatca ccatggcgaa cggccggatc acttctccgc cgaagaccac cagggcgagc
     2341 gtggccgcca tcgtcgtccc gtgggtcagg atcgagcgag gcagggtctc gttgatcgac
     2401 aggttgagga tctcgtagag attctggcgc cggaacttgc gcaggttctc gcgcacccga
     2461 tcgaagatga tgatcgtgtc attgagcgag taccccacca tggtaaggac cgcgcccacg
     2521 acgaccaggc tcacctcgag attgaagtag cggatgaacg cgatcgtggt gagaatgtcg
     2581 tggagcgtgg cgagcacggc cgccagtccg aagcgccact cgaagcggat cgccagataa
     2641 atcaacgtca cgacgaacga gaagaggatg gcgaggaacg ccttcccctg cagctcccgg
     2701 ccgaccttgg ggcccaccgc ttcgtggcgc tcgacccggt agctccccac accgagcttc
     2761 gtatccagga cgcgctgcac ggccgccgtc gtctcctcgg tgccgcctcc gggggtcgtc
     2821 gcctgcgcgc ccagccgcgc gcggatgatg taggaggtgt cggacccgaa cgactggatc
     2881 tccgttccgg gaatccccgc cgcgtccagg tcggcccgca gctcggacgt cgggaccgga
     2941 cgcgccgttt cgatctggat cagggtcccg ccggtgaact cgatgctgta gttcaggccc
     3001 ttcaccgcga gcaggatgag cccgggcacg atcacggtcg ccgtcagggc gtaggcccag
     3061 cggcgccact tgatgaaatc gtaactggcg ttggcgaaga atcggatcat cagacgctca
     3121 gggtttgcat gtcgggccgc tggcgcagcc agacgaggta gaaggtgcgg accacgaaga
     3181 tggcggtcac catcgacgcc gcgatgccga tgatcagcgt gatggcgaag ccctgcaccg
     3241 ggccggtgcc cacgaggtag agaatgaacg cggtcagcgc ggtcgagacg ttggagtcga
     3301 tgatcgcgct catggcgtgc ttgaagcctt catccaccgc cagccggacc gtcttgccgt
     3361 gctgcagctc ctcgcggatg cgctcgaaga tcagcacgtt ggcgtccacc gcgatgccca
     3421 ccgacagcac gagacccgcg agccccggca gcgtgagggt gaaaccgaag acggacaacc
     3481 cggcgagcgt caacaccacg tagaggccca gtgccgatac ggcgagcacg cccgacagac
     3541 ggtagtacac gaccatgatc aggatgacga ggaccacgcc cacgatcccc gcccggatcc
     3601 cgtcgtggat cgagtcgctg ccgaggctgg gtccgatggt acgctcctcg atgatctgca
     3661 gcggcgcggg cagagcgccc gcccggagca ccagggcaag gtcctgggct tcggtgagcg
     3721 gcttgttctg caggtcgatc tggccggtgc cgcagatctg atcgttgatg atcggcggct
     3781 gtccctgcac ccggttgtcg agcatgatgg ccatgtaatc gtggacgtgg cggccggtct
     3841 cctcgcagaa cttgcggctc ccccggcggc tcaggtcgaa tacgaccacc gaatgattgt
     3901 tcagctgatc ccgccgcgcg ctcgccttgg tcagctcctc gccggtgatg atcggccggt
     3961 tctcgaccgc atagagcgcg cgataggagc gggcgctgcg gacctcggtg gcggtgcccc
     4021 acttcaggtc gaggccgcgc ggcacggcag cctgcacctc cggccgcgcc agcaggctct
     4081 cggcgcgcgg cacctgctct tcgggcacca ggaactcacc cggcatctgc ccctggaaca
     4141 gcagcgccga gagtgccccc ggcgcgttcg cgtcgggcgt cgtgtccttg gccgcgccct
     4201 tcttcgtcgt gtccgcgccg aagagctggc tgaccgcgct cacctgcgcg cccgtcggcc
     4261 ccttgacccc cgcctggcgc aggacgttgt cgatcgccgg cagcgcgtcg cgaaacaggt
     4321 tcttcatgtc ggtgatgcgg aattcgagga aggcggtctg catcacgatc tgcttcgcgc
     4381 gcgcggggtc cttctccccc gccagctcca ccaccagccg gcaattcccg accacctgca
     4441 ccaccggctc ggtggtgccg aactcgtcca cccgcgtgcg cacgacgcgc tcggcgcggc
     4501 ggatcgcgtc cgcgcaatcg gccaccggtc ccttcgactg atccacctcc agcgccaggt
     4561 ggatcccgcc ctggagatcg aggccgaggt tgatgggcac ccgccggatg gtcgtgtcct
     4621 tcatccggcc cgtctgcttg tcggggacgc ggatcttcgc gtccttgggc agcaggaacc
     4681 agactgatat caagagcaac ccgacaatcg tgatgatgcg accacgaatc ccgctcatct
     4741 gacactcact cgctcaagag tctcgcgcgc cgccgccgcg tcccgggcca gctgctccac
     4801 cagcgcatcg cgcgaggcga aggtctggac gtcgcgcagg cgccgcaccc actccaccca
     4861 caccgtttca ccatagaggt cgtccgtgaa gtcgaacaga tggacctcca ggccgcgctc
     4921 ggccacaccg aacgtcggcc gcgacccctg gttcatcata ccgccgtgac ggcctcgctc
     4981 cacccgtccc tcggcccccg tcccccgtcc ctcgaccgtc acccacacgg cgtatacccc
     5041 gtcgggtggg agcagcttgc gcccgtccgg caccgggagg ttgatcgtcg gcaccccgat
     5101 cgtccgcccg cgtcccgccc ctcgcgtgac ctcccccagc gccgcgtacg gccgcccgag
     5161 ccagcgcgcg gcgagcgcca ggtccccatg ggccaccgcc gcgcgaatca gcgtggacga
     5221 gatcggctgt ccatcctccc gaaccgcgtc caccacgtcg acgagaaaac cgtcggcggc
     5281 cccgatgcgg cggaccacct ctacgtcacc cgcgcgacca cggccgaacg catgatcgtg
     5341 ccccagcacc aactcgtcca tcccgacctg gagccgcagc acgtcgcgca cgaactcctc
     5401 gggtcccagc cgggacagct gaggcgtgaa cggcagcgtc accacccggt ccacgccgtg
     5461 ctccatgagc agccgttgcc gctcagccgg cagcgtgagc aggcgcggcg ccgcggcggg
     5521 attcacgacg gcgagcggat gcggatcgaa cgtgaccacc acgctctcgg ctccgcgccg
     5581 ccgcgcccgt gccacgattt cggcgagcac cgcctgatgg ccgcggtgca ccccgtcgaa
     5641 ggtcccgacc gtgacgaccg cgctcactcg gtcaccacga cccgcggctt gagcacgtcc
     5701 ccttcggcct ccgccaccgc cacgagccgg cccgcgtgca gcagggccac ctgcgggcca
     5761 tcctgagccc gcgccgggat cgcgcgcccg tgcaccacgg ccacgcgctc cgcttcgtcc
     5821 agctcccggc tcggcagcga tgccacgagc gacactgcgg gacgcagatc cgccgccgtc
     5881 actgcctccg ccgcccgggc gtcctccagg cggaacggcc ccaccgcggt ccgcgtcagc
     5941 gtcaccaggt gggcgccgca gccgagcgcc tcccccacgt cgcgggcga
//
LOCUS       DBNGZW010000757         4345 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1020034, whole genome shotgun sequence.
ACCESSION   DBNGZW010000757 DBNGZW010000000
VERSION     DBNGZW010000757.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1020034"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3057
                     /locus_tag="AC16JC_17680"
     CDS             <1..3057
                     /locus_tag="AC16JC_17680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013762650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR5166588.1"
                     /translation="ALAPTDAEEIVLRRYGRPGTVRPLPSERDQNFLVRFADGKRAVL
                     KIAHAGEDRDVLELQHAALRRLSERAPGIALPRVLLTADGSEIVEVPGAEGEAHQVRL
                     LSWVPGAVLALIRPHTPELLESLGRLIAQLDTGLEGLDHPAARRALKWDLARAEWIGD
                     HLDAVSDPARRALVTRALDRYRADVMPALGGLPAGMIYNDANDHNVLVRGEDPWTRRV
                     TGAIDFGDLVHSAIVCDVAIAASYAALGKPDPLAAAARVVAGYQALHPLSEAEIDLLD
                     ALIETRLTVSVVNSAVQRAALPDNAYLTVSEAPAWETLERLTRLPPRFARCVLRDACG
                     FSAVPTAPAVVQWLTDHPDELAPVMEVDFARGAAVLDLSVGSPAIANPHDIASVPAFV
                     GTVAAALAEAGKTIGIGRYDEPRLLYTTDAFRAEGNDGPEWRTVHIGLDLFLPPGTPV
                     QAPLGGMVHSFRDNAAPGDYGPTIILRHTVDGGRLRFFTLYGHLDRDSLAWLRPGVSV
                     APGTVIGRLGEADVNGGWPPHLHFQLVTDLLDLEGDFPGVARPSQRRVWKSLSPDPNL
                     IARVPSALFPAPPPTLERLLDERRHHLGPSLSIAYRRPVEVVRGWMEFLYDREGRRYL
                     DAVNNVPHVGHGHPRVVRAGQRQMALLNTNTRYLHELAVRYAERLWATLPSSLSVCYF
                     VNSGSEANELALRLARAYTGRRDVIVVESGYHGNTTTLVDVSPYKHDGPGGAGAPSWV
                     HKVPLPDTYRGRFRRDEPLAGERYAAFLADAIARIRARGAQPAAFLCESVPSCGGQIV
                     LPEGYLTAAYGHVRRAGGICIADEVQTGFGRIGSHFWAFEAQDVVPDIVTLGKPMGNG
                     HPLGAVVTTPEIAAAFANGMEYFSTFGGNPVSCAIGLAVLDVIADEGLQENARLIGER
                     VQAGLRSLASRHPPIGDVRGLGLFLGVELVRDRTTLEPAGEEAGYVASRMRERGVLVG
                     TDGPFHNVLKIKPPLVFTREDADHLVATLDEVLSEGLSHRRADEEG"
     gene            3125..3277
                     /locus_tag="AC16JC_17685"
     CDS             3125..3277
                     /locus_tag="AC16JC_17685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166589.1"
                     /translation="MAGKGRRTTHRSSSGKKLYAVRDAKGRFKDIQSYKRAHGADIKR
                     KSKKER"
     gene            3274..3735
                     /locus_tag="AC16JC_17690"
     CDS             3274..3735
                     /locus_tag="AC16JC_17690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166590.1"
                     /translation="MRAGRAAAGFVLALLACSGDTSRLTPELQARLAAEGQLHRADNI
                     WFRFTHDAGTRSAGWEDRRGSIIVTRQTVLIHKNEKIGIEITAASRRAYDVTRDGDRV
                     RIGAGSGKSREVWSFAPPDDAEGWVRDIRAVIRLARGEPADAQGPRSRGAR"
     gene            complement(3713..>4345)
                     /locus_tag="AC16JC_17695"
     CDS             complement(3713..>4345)
                     /locus_tag="AC16JC_17695"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor domain-containing
                     protein"
                     /protein_id="HIR5166591.1"
                     /translation="QFGYKVIPNAVLRSERAWSEEAGVTATPTARLRLDGTVFQNQYR
                     DLIGPVVLLCPVTPTPPCPPGVSGFVAQFRNVTRARVRGLDLSVQSRILGDLLDLEAS
                     YLLLATRDDSTGAPLPYRSRHNISGTLSAFRGLVDVDVRYRSRVEQVLQYFNDARGDF
                     TVVDLRMAYRVAGIVLQAKVTNLFQALYVDVQERNPGAPRQVSLTALRDF"
BASE COUNT          662 a         1553 c         1483 g          647 t
ORIGIN      
        1 gccctcgcac ccacggatgc cgaggaaatc gtactgcgcc gctacgggcg ccccggtacg
       61 gtgcgcccgc tgcccagtga gcgcgatcag aacttcctgg tccgcttcgc cgacggcaag
      121 cgggcggtcc tgaagatcgc gcacgccggc gaggatcgtg acgtgctcga gctccagcac
      181 gccgccttgc gccgcctctc ggagcgcgcg cccgggatcg ccttgcccag ggtgctcctc
      241 accgcggacg gctcggagat cgtggaggtc cccggcgccg aaggagaggc gcatcaggtg
      301 cgcctgctgt cctgggtgcc gggagctgtg ctcgctctga tccgtccgca tacgcccgag
      361 ctgctcgaga gcctggggcg gctcatcgca cagctcgata ccggactcga gggactggac
      421 catcccgccg cgcgccgggc gctgaaatgg gatctcgccc gcgcggagtg gatcggagac
      481 catctcgacg ccgtgagcga cccggcccgg cgcgccctgg tgacccgggc gctggatcgc
      541 taccgggccg acgtgatgcc cgcgctcggc ggcctgccgg ccgggatgat ctacaacgac
      601 gccaacgacc acaacgtcct cgtccgcggc gaggatccct ggacccgacg ggtgaccggc
      661 gcgatcgact tcggcgacct ggttcacagc gccatcgtgt gcgacgtggc catcgccgct
      721 tcgtacgccg ccctcggcaa gcccgacccg ctggcggcgg ccgcacgcgt cgtggcgggc
      781 taccaggcgc tgcatccact gagcgaggcg gagatcgatc tgctcgacgc cctgatcgag
      841 acacggctca ccgtcagcgt cgtcaattcc gcggtgcagc gggcggccct tcccgacaat
      901 gcctatctga cggtcagcga ggcaccggcc tgggagaccc tcgagcgcct cacccggctg
      961 ccgccgcgtt tcgcccgctg cgtactgcgg gacgcctgcg gcttctccgc cgtcccgacc
     1021 gcgcctgccg tcgtccaatg gctgaccgac catcccgacg agctggcccc ggtgatggag
     1081 gtcgatttcg cgcgcggggc ggccgtgctc gacctctcgg tgggcagtcc ggcgatcgcc
     1141 aatccgcacg acatcgcgag cgtccccgcg ttcgtgggga ccgttgcggc cgcactcgcg
     1201 gaggcgggaa agacgatcgg catcgggcgc tacgatgagc cgcgcctcct gtacacgacc
     1261 gacgcgttcc gcgccgaggg gaacgacggg ccggagtggc gcaccgtgca catcgggctc
     1321 gatctcttcc tgccgcccgg cacgccggtg caggcgccgc tcggcggcat ggtgcactcg
     1381 ttccgcgaca atgcggcccc cggcgactat ggtcccacca tcatcctgcg ccacacggtg
     1441 gacggcggac ggctccgctt cttcacgttg tacggtcacc tggaccgtga ctcgctcgcc
     1501 tggctgcgcc ctggcgtttc cgtggctccc ggaacggtca tcggtcgact cggggaggcg
     1561 gacgtaaacg gcggctggcc gccgcacctg cacttccagc tcgtcaccga tctcctcgat
     1621 ctcgaggggg actttccggg cgtggcgcgc cccagtcaac gccgggtgtg gaagagcctc
     1681 agccccgacc ccaatctgat cgcccgcgtc ccctcggccc tgtttccggc accgccgccg
     1741 acgcttgagc gcctgctgga cgagcgccgc catcacctcg ggccgtccct cagcatcgcc
     1801 taccgccggc ccgtcgaagt cgtgcgcggc tggatggagt tcctttacga ccgggagggg
     1861 cgccgctatc tcgacgcggt gaacaacgtt ccccatgtgg ggcacgggca cccgcgggtc
     1921 gtgcgcgccg gccagcgcca gatggcgctg ctgaacacca acacgcgcta cctgcacgag
     1981 ctggcggtgc gttacgctga acggttgtgg gccaccctgc cgtcgtcact gtcggtgtgc
     2041 tacttcgtca attccggcag cgaggcgaac gagcttgcgc tgcgcctcgc ccgtgcctat
     2101 accggacgcc gcgacgtcat cgtggtcgaa agcggttatc acggcaacac gacgacgctc
     2161 gtggatgtga gtccctacaa gcacgacggt ccgggcggcg cgggagcgcc gtcctgggtg
     2221 cacaaggtcc cgctcccgga cacctatcgc ggccgattcc gccgcgacga gccgctcgcg
     2281 ggggaacgct acgccgcctt cctggcggat gcgatcgccc gcatccgggc gcgcggcgcg
     2341 cagcccgccg ccttcctgtg tgaatcggtc ccgtcgtgcg gggggcagat cgtcctgccc
     2401 gagggatacc tgaccgccgc ctacggccac gtgcgccggg caggaggtat ctgcatcgca
     2461 gacgaggtgc agacggggtt cggccgcatc ggctcgcatt tctgggcgtt cgaggcgcag
     2521 gacgtggtgc ccgacatcgt caccctcggc aagccgatgg gtaatggcca cccgctgggc
     2581 gccgtggtca ccacgccgga gatcgccgcc gcgttcgcca acggcatgga gtacttcagc
     2641 acgttcgggg gcaatcccgt gtcctgcgcc atcggcctcg cggtgctgga cgtgatcgcc
     2701 gacgaagggc tgcaggagaa tgcccgcctc atcggggagc gcgtgcaggc aggattgcgc
     2761 tcgctcgcat cccgtcaccc tccgatcggc gacgtgcgcg gcctcggtct cttcctgggc
     2821 gtggagctgg tgcgcgaccg caccaccctc gagccggccg gcgaggaagc ggggtacgtc
     2881 gccagccgca tgcgggaacg cggcgtgctg gtcggcaccg acggcccgtt ccacaacgtg
     2941 ttgaagatca aaccgccgct cgtgttcacc agggaagacg cggaccacct cgtcgccacg
     3001 ctcgacgaag tcctctccga agggctgagc catcgacgcg cggacgagga ggggtagccc
     3061 gcacgcagca aaccttgcag cttattggcg tagccctgca ggcatctcga atcggggagg
     3121 caccatggcc gggaaaggtc gccgcaccac ccatcgcagc agctccggga agaagctgta
     3181 cgcggttcgc gacgcgaaag ggcgcttcaa ggacatccag agctacaagc gggcgcacgg
     3241 cgccgacatc aagcgcaaga gcaagaaaga gcggtgaggg cggggcgcgc cgccgccgga
     3301 ttcgtcctgg ccctgctggc ctgctccggc gacacgagcc gtctcacgcc ggagctccag
     3361 gcccgcctcg cggccgaggg tcagctgcac cgcgccgaca acatctggtt ccgctttacc
     3421 cacgacgccg gcacgcgctc ggcgggctgg gaggatcgcc ggggctcgat catcgtgacc
     3481 cggcagaccg tcctgatcca caagaacgaa aagatcggca tcgagatcac cgcggccagc
     3541 cgtcgcgcct acgacgtcac ccgggacggc gatcgcgtcc gcatcggggc ggggtcgggg
     3601 aagtcccgag aggtctggtc cttcgccccg ccggatgacg cggagggctg ggtgcgggac
     3661 attcgggccg tgatccgcct cgcgcgcggc gaacccgccg acgcacaggg ccctagaagt
     3721 cgcggagcgc ggtgagactc acctggcgag gtgcgcccgg attgcgctct tgcacgtcca
     3781 cgtacagggc ctggaagaga ttggtcacct tcgcctgcaa tacgatgccc gccacccgat
     3841 atgccattcg caaatccact accgtgaagt cgccgcgcgc atcgttgaag tactgcagca
     3901 cctgctccac ccggctccgg tagcgcacgt ccacgtccac caggccgcgg aaggccgaca
     3961 gcgtgccgct gatgttgtgg cgcgagcggt agggcagagg ggcgcccgtc gaatcgtccc
     4021 gcgtggcgag caggagatag ctcgcttcca ggtcgagcag atccccgagg atccgggact
     4081 gcacgctcag atccagcccc cggacgcgcg cccgcgtgac gttccggaac tgcgccacga
     4141 atcccgacac gcccggaggg catggtggtg tgggcgtgac cgggcagagc agcactaccg
     4201 ggccgatcag gtcacggtac tggttctgga acaccgtgcc gtcgagccgg agccgggctg
     4261 tcggcgtcgc cgtgactccc gcctcttcgg accaggcccg ctcgctgcgc agaacggcat
     4321 tgggaatcac cttgtagccg aactg
//
LOCUS       DBNGZW010000758         3788 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1763786, whole genome shotgun sequence.
ACCESSION   DBNGZW010000758 DBNGZW010000000
VERSION     DBNGZW010000758.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3788)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3788)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3788
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1763786"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="AC16JC_17700"
     CDS             complement(<1..205)
                     /locus_tag="AC16JC_17700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HIR5166592.1"
                     /translation="MARIDVLMPQMGESIAEGTLSKWLKRLGDTVKRDEPLFEISTDK
                     VDAEIPAPTAGTLVEIKVQEGQTV"
     gene            complement(291..1268)
                     /locus_tag="AC16JC_17705"
     CDS             complement(291..1268)
                     /locus_tag="AC16JC_17705"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR5166593.1"
                     /translation="MAEVTFLEALRQGLWEEMERDERVFMLGEDIGAYGGAFKVTDGF
                     QKKFGEARVIDTPISEAAIVGAAAGAAHMGMRPVAEMQFIDFISCAYDMLTNYVATAR
                     YRAGLATPIVVRGPCGGYVRGGPFHSQNPEAAFLHTPGLKIVYPATARDAKGLIKASI
                     RDEDPVLYLEHKWLYRRIKETLPEGEEVLTPIGQARLARDGKDLSVITYGATVWKALE
                     AADQLAQEGVSVEVLDLRTLLPLDDDAIVATVKRTGKVLIVHEDTRTGGLAGEITARI
                     NERAFEWLDGPIRRVTAHDVPLPYAPTLEDFVLPQTDDIVNAARWLAKY"
     gene            complement(1268..2329)
                     /locus_tag="AC16JC_17710"
     CDS             complement(1268..2329)
                     /locus_tag="AC16JC_17710"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR5166594.1"
                     /translation="MPPHSVPDTRSDTPAIPADLERLQLLEMYRYLRLTRVLEERLVA
                     LYRQSKVIGGLFRSLGQEGESVAAAFALERGRHRDILSPLIRNLGALLVMGAKPIEIL
                     RQYMAKSEGPTRGRELNVHFNDLEKGYLGQISHLGDMVPVMAGITLTFRMRGESRVGL
                     VFVGDGATSTGAFHEGLNFAAVQRCPLVVIGEYNHWAYSTPPEKQFAVPDLADKAKGY
                     GIHAVTVDGNDVLAVYAATKYAVERARRGKGVHFIEVKTYRRKGHAEHDDQSYQPKDE
                     IERWARENDPVDRLAKQVLDNDGAEAAELAEIDRAVKDEIDQATDACIDEPLPAPESA
                     LPGVYAQPAAAERLWFRSL"
     gene            2405..2620
                     /locus_tag="AC16JC_17715"
     CDS             2405..2620
                     /locus_tag="AC16JC_17715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017870256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HIR5166595.1"
                     /translation="MASAKLKVTGMSCGHCQKTVEQALKGVTGVYSAVVDLQDGEAEI
                     DFDDDAVTAGALVAAVEKAGYRARLAG"
     gene            complement(2628..2918)
                     /locus_tag="AC16JC_17720"
     CDS             complement(2628..2918)
                     /locus_tag="AC16JC_17720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166596.1"
                     /translation="MRYFHRTPLSPADVLAAARDYFGARLAPSEESPRRLGYRGTLGK
                     VTVSARAEGGHYTFIDILTDQVGESEIDKLAKRFLAEVHRTVEPAHELRGAY"
     gene            complement(2959..>3788)
                     /locus_tag="AC16JC_17725"
     CDS             complement(2959..>3788)
                     /locus_tag="AC16JC_17725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016879823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HIR5166597.1"
                     /translation="IAQSVLRAAQERLVQLLPVEKFAAMEGRGVRGTVERRIVEVVSA
                     RHARERSLELGSLVQDADRLADQGRTPVIVVVNNTVHAVIGISDPVKPTAKDAVARLR
                     GLGVEVRMVSGDTRRSALVVAAEVGIDEVSAEVLPSQKADLVKQEQERGVVVAMVGDG
                     INDAPALAQADVGIAIGTGTDIAMEASDVTLIRGDLRGVVAALEISRRTLGTIRQNLF
                     WAFLYNVLGIPIAAGALYPLTGLLLSPVIARAAMAWSSLSVVLNSLRLRRYTPAWVP"
BASE COUNT          589 a         1326 c         1237 g          636 t
ORIGIN      
        1 gaaccgtctg gccttcctgc accttgatct cgaccagcgt ccccgcagtc ggggccggaa
       61 tctcggcatc caccttgtcg gtggagatct cgaacaacgg ctcgtcccgc ttcacggtgt
      121 cgccgagtct cttgagccat ttgctgagag tcccttcggc gatggactct cccatttggg
      181 gcatgaggac gtctatgcga gccatagtat ctccgatgaa gcgctatcgg ctatcggcca
      241 tcagctatcg gcagtactga tggccgatag gtgatagctg atagcgtctc tcagtatttc
      301 gccagccatc tcgccgcgtt gacgatgtcg tccgtctgcg gcagcacgaa atcttccagc
      361 gtcggcgcat aggggagcgg tacgtcgtgc gccgtcaccc gccggatcgg gccgtcgagc
      421 cattcgaacg cccgctcgtt gatgcgcgcc gtgatctcgc ccgccagccc gccggtccgg
      481 gtatcctcgt ggacgatcag caccttcccc gtcctcttca ccgtggcgac gatcgcgtcg
      541 tcgtcgagag gcagcagcgt gcgcaggtcc agcacttcga ccgacacgcc ctcctgcgcc
      601 agttggtcgg ccgcctctag cgccttccac acggtcgccc cgtaggtgat caccgacagg
      661 tccttcccat cccgggcgag ccgcgcctga ccgatcgggg tgagcacttc ctcaccttcc
      721 ggcagcgtct ctttgatgcg gcggtacagc cacttgtgct cgagatagag gacaggatcc
      781 tcgtcccgga tcgaggcttt gatgaggccc ttcgcgtcgc gcgccgtcgc cggatagacg
      841 atcttcaggc cgggcgtgtg caggaacgcc gcctcgggat tctgcgaatg gaacgggccg
      901 ccgcgcacgt acccgccgca gggcccgcgc accacgatcg gcgtggcgag gccggcgcga
      961 tagcgcgcgg tcgccacgta gttcgtcagc atgtcgtagg cgcaggaaat gaaatcgatg
     1021 aactgcatct ccgcgaccgg ccgcatcccc atgtgcgctg cgcccgccgc cgcccccacg
     1081 atcgcggctt ccgaaatcgg ggtgtcgatg acgcgcgcct cgccgaactt cttctggaac
     1141 ccgtcggtca ccttgaacgc gccgccgtag gccccgatgt cctcgccgag catgaacacg
     1201 cgctcgtcgc gctccatctc ctcccacagt ccctggcgca gggcctcgag gaacgtgacc
     1261 tcggccatca cagactccgg aaccacaacc gctcggcggc cgcgggctgg gcatagacgc
     1321 cgggcagcgc cgattcgggc gccggcagcg gctcgtcgat acaggcgtcg gtcgcctgat
     1381 cgatctcgtc cttcaccgcg cggtcgatct cggccagttc ggccgcctcg gccccgtcgt
     1441 tgtcgagcac ctgcttcgcg agccggtcga cgggatcgtt ctcccgcgcc cagcgctcga
     1501 tctcgtcctt cggctgatag gactgatcgt cgtgctcggc gtgccccttg cggcggtacg
     1561 tcttcacctc gatgaagtgg acccccttgc cgcgacgcgc gcgctccacc gcgtatttcg
     1621 tcgctgcgta cacggcgagc acgtcgttgc cgtccaccgt gaccgcgtga atcccgtagc
     1681 ccttggcctt gtcggcgagg tccggcaccg cgaactgctt ctcgggcggc gtggaatagg
     1741 cccagtggtt gtactccccg atgaccacga gggggcagcg ctgcaccgcg gcgaagttca
     1801 acccttcgtg aaacgccccg gtcgacgtcg cgccgtctcc cacgaacacc aggccgacgc
     1861 gcgactcgcc gcgcatcctg aaggtgaggg tgatcccggc catcacgggg accatgtccc
     1921 ccaggtggga gatctgcccc agatacccct tctcgagatc gttgaagtgg acgttcagct
     1981 cgcgtccgcg ggtggggccc tccgacttgg ccatgtactg gcgcaggatc tcgatcggtt
     2041 tggcgcccat cacgagcagt gcgccgaggt tgcggatcag gggcgagagg atgtcgcgat
     2101 gacggccgcg ctccagcgca aaggccgccg ccaccgattc cccctcctgc cccagggagc
     2161 gaaacagccc cccgatcacc ttgctctgcc ggtacagcgc gaccagccgc tcctccagca
     2221 cccgggtgag gcgcagatag cggtacatct cgagcagctg gagccgctcg aggtcggccg
     2281 ggatggccgg cgtgtccgac cgggtgtcgg gtacggagtg gggaggcatc agctaaatta
     2341 tagtgggcgg gaaagttgac ccctggggag aggcaaatca agctgtggcg gagggaccgc
     2401 acctatggcc agcgcgaagc tcaaagtcac cggcatgtcc tgtggacact gccagaagac
     2461 cgtggagcag gccctgaagg gcgtgacggg cgtgtattcg gcggtggtgg atctccagga
     2521 cggcgaggcc gaaatcgact tcgacgatga tgccgtgacg gccggggcgc tggtggccgc
     2581 ggtggagaaa gcggggtacc gcgccaggct cgccggctga ctccgcttca gtacgccccc
     2641 cgcagctcgt gcgccggctc gaccgtgcgg tgcacctccg cgagaaatcg tttcgcgagc
     2701 ttgtcgatct ccgattcccc gacctggtcg gtaaggatgt cgatgaaggt gtagtgcccg
     2761 ccctcggcgc gcgcggagac ggtcaccttc cccagcgtgc cgcggtagcc cagacggcgc
     2821 ggcgactcct ccgacggcgc gagccgcgct ccgaaatagt cccgcgcggc ggccagcacg
     2881 tcagccggcg acaacggcgt tctgtgaaaa tatctcatcg gactcccctg gcttcggtgc
     2941 ggctcgacgg ccgcgcgtct acggaaccca cgccggagtg tagcgccgca ggcgcaggct
     3001 gttcaagacc acgctgaggc tggaccacgc catggcggct ctggcaatca ccggcgagag
     3061 cagcaggccg gtcagcgggt acagcgcccc cgcggcgatc gggatgccca gcacgttgta
     3121 gaggaacgcc cagaagaggt tctgccggat ggtgcccagc gtccggcgtg agatctcgag
     3181 ggcggcgacc acgccgcgca gatcgccgcg aatcagcgtg acgtctgacg cttccatggc
     3241 gatgtcggtc ccggtgccga tcgcgatgcc cacgtcggcc tgcgccagcg ccggcgcatc
     3301 gttgatgccg tcgccgacca tggcgacgac gacgccccgc tcctgctcct gcttcacgag
     3361 atcggccttc tgggagggca gcacctcggc gctgacctcg tcgatgccga cctccgccgc
     3421 gaccaccagg gcagagcggc gcgtgtcgcc ggagaccatc cggacctcca cgcccaggcc
     3481 gcgcagtcgc gccacggcgt ccttggcggt cggcttgacc ggatccgaaa tcccgatcac
     3541 ggcatgtacg gtgttgttca ccacgacgat caccggcgtg cgtccctggt ccgcgagccg
     3601 gtcggcatcc tggaccaggc tgccgagctc gaggctgcgc tcacgcgcgt gccgcgccga
     3661 gacgacctcg acgatgcgcc gttcaacggt gccgcggacc ccgcggccct ccatggcggc
     3721 gaatttttcg accggcagga gctgcaccag ccgctcctgc gccgcccgga ggacggactg
     3781 ggcgatcg
//
LOCUS       DBNGZW010000759        10131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1082178, whole genome shotgun sequence.
ACCESSION   DBNGZW010000759 DBNGZW010000000
VERSION     DBNGZW010000759.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 10131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10131
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1082178"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..296)
                     /locus_tag="AC16JC_17730"
     CDS             complement(<1..296)
                     /locus_tag="AC16JC_17730"
                     /inference="COORDINATES: protein motif:HMM:NF013703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HIR5166598.1"
                     /translation="MSTAAAPPGTPGVLTIGPLRPVILRLALPAVAMMACHFTFGVID
                     AVWVGRLIGPAALAAVSTAGFYIWILLNLGEMVEVGLIAVAARRHGEGAPDRAAR"
     gene            complement(293..1060)
                     /locus_tag="AC16JC_17735"
     CDS             complement(293..1060)
                     /locus_tag="AC16JC_17735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166599.1"
                     /translation="MRLLTAALLLLQAADGGRRAELRAGLDTLYAGYFAAAARYFADL
                     AARDTADPAPLTFEAGAYIWWAAAKDSDDFAASRIDSLLDRAIELGKVRAPGIERDFW
                     LGTALGYRARERELHGHNWGAARDAKAMRDAYRRVLAADSSCTDCFLGLGLYSYGLAR
                     ASTLAKIVGRIIGLGGGNADEGLAMMRRVAQSGDLARVEGSWVLAAALVREANRNERT
                     GAGRRAEAVGLVNGLAGRYPANPVFQRFLHEMAHRPP"
     gene            complement(1062..2147)
                     /locus_tag="AC16JC_17740"
     CDS             complement(1062..2147)
                     /locus_tag="AC16JC_17740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HIR5166600.1"
                     /translation="MPNEIQQRVSAALAALAQPDRIHDLAVDDAGQVSFTFHLKREDP
                     ATLVRDARRAVQALEGVTGVKISVVDSATPAGPASRSGTGPGRASSPVPPPPSPEDMP
                     RLGRALAISSGKGGVGKSTVSVNLAVALARAGHAVGLMDADIYGPNIPRMMGVDEKPP
                     VQGGKIQPLEAHGVKLMSLGFIVERDQPAIWRGPIIMKIIHQFLHDVDWGDLDFLLVD
                     LPPGTGDAQLSLVQTIHLHGALIVTTPQEVATGDSLRGAKMFERVGTKVLGVVENMSY
                     YVCEHCGERSELFLSGGGERLAAELGVPLLGQIPLQAGLARLADGGQPIVVSQPDSPA
                     GKALAHTARQVAALLQSAPSGPLPVTR"
     gene            complement(2169..2348)
                     /locus_tag="AC16JC_17745"
     CDS             complement(2169..2348)
                     /locus_tag="AC16JC_17745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166601.1"
                     /translation="MGLLGFYRTLRDERRQLGWKGLLKRRGWAFVAVVVLVYLVRDLV
                     LYVLVPLAVVAGLRH"
     gene            complement(2348..2677)
                     /locus_tag="AC16JC_17750"
     CDS             complement(2348..2677)
                     /locus_tag="AC16JC_17750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014586402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR5166602.1"
                     /translation="MRAARESLTRLEQIRNVGPSLAADLRLVGVRFPADLRGRDPYAL
                     YDALCAATGERQDPCVLDVFISAVRFMEGAPARPWWHYTKERRRVLQARSAALPCDNG
                     PPGSSFP"
     gene            complement(2674..3087)
                     /locus_tag="AC16JC_17755"
     CDS             complement(2674..3087)
                     /locus_tag="AC16JC_17755"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR5166603.1"
                     /translation="MAAAIRLTRLTPVLIVDSVEPCLAFWVGRFGFEAKNKVPGPDGK
                     LVFASVEKDGIEVMYQTRASVLADSPGSASELVGHSTVLFITVPDLDAIERAVAGAPI
                     VKPRHKTFYGSTELYVREPGGNTVGFAQFSEEAGA"
     gene            complement(3182..3616)
                     /locus_tag="AC16JC_17760"
     CDS             complement(3182..3616)
                     /locus_tag="AC16JC_17760"
                     /inference="COORDINATES: protein motif:HMM:NF022591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HIR5166604.1"
                     /translation="MNSMLSLLVFAAVAGPLVAQDSTKAAAAPAAPAAGALSVAVGVV
                     ARSVADRAPQDTGAAFPADVGGLVCFTQIEGASADAPGTIHHVWFHGDQQVADVELAA
                     RGSPWRTWSRKTVPAEWTGPWHVEIRDAGGAVLKRIDFTVGQ"
     gene            complement(3708..5780)
                     /locus_tag="AC16JC_17765"
     CDS             complement(3708..5780)
                     /locus_tag="AC16JC_17765"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HIR5166605.1"
                     /translation="MQTRVACTLTLCGMLGMVPSTGRSQTAARYPDRAVRRDIPMTGM
                     IRHALAAGTRDSTGRPGRNYWQLWTDYTISARFDPATGVLTGHERVLVQNAGDTVMSR
                     IVLRLDQNLFAANVPRAEVVTQITGGMQLTALSVDGNAVNLTPPPRVFRRGPNGEPPP
                     VILAAYDTAQTVANVTLPNPVPAHGSVTLEADWQFTVPKVAPQTRGVRMGAWGDSLYQ
                     VAQWYPRVAVFDDLRPGGWDTDPYLGPSEFYNNFGRFDVKLDLPAGWLVGATVLLQNP
                     AEVLSAVERERLTHVLAADSTVRISGPDELGPGKATAAGDRLVWHFVADTAGDVAWAA
                     SNQFVWDATHADIPGKGAIPIDIMYLPGHERQYADAGPTVRHALEFYSRLWIPYAFPI
                     LTMVDGPEGGMEYPMFIMSGTGASDHETGHQWWPMMLGVNETWYGFMDEGFNQYMNIL
                     SRADRQHQPPNLDGVGQAYGAVSGNEREAPLMWDANYGGPMYGFQAYLKAPMMLSMLG
                     GVVAGGDSAVWRAMREYAGTWRFKHPSPWDYAFFMSNALHQELGWFWYSWLFTTDAVD
                     GSIAEVRTAGSRTTVTVRQDGQMPSPVVLAVHFAPGGPAPKKMTNATLVDDSTAIVTW
                     PVDAWFGGSRTFEARLDFGARKIARVVLDPHCRFPDRNVDDNTWPRQPAPVAAADANP
                     FRAPACKG"
     gene            5857..6594
                     /locus_tag="AC16JC_17770"
     CDS             5857..6594
                     /locus_tag="AC16JC_17770"
                     /inference="COORDINATES: protein motif:HMM:NF015709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family acid phosphatase"
                     /protein_id="HIR5166606.1"
                     /translation="MRLATMLAVGSLAACGTPARPAPATAPTPARAPAPLPAPLRWMR
                     RSAEYRALTRQIYAFAALRLDELARGRPAGSWAVILDADETVLDNTEYERRRASVDSG
                     YSDASWASWVRERAAGAIPGAAAFTATVRGLGGRVVIVTNRADSLCADTRANLVTAGV
                     ATDLVLCMPPGESDKNPRFERVQQGTAAPGVAALTVVEWIGDNIQDFPHLTQDVRSDS
                     TALAAFGRRFFVLPNPVYGSWERNQEP"
     gene            complement(6601..9006)
                     /locus_tag="AC16JC_17775"
     CDS             complement(6601..9006)
                     /locus_tag="AC16JC_17775"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014101391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR5166607.1"
                     /translation="MRHTFRWALLLLLLPAPIGAQERYRTPPQAIVDILDAPPLPAAT
                     LSPDRQWLLLLEQPSMPTIAEFAAPMLRLAGWRIDPRTNGPARGQRITGLVLKRVADG
                     LERRIPLPAGAVVGDVDWAPDARHFAFTNTRPDGIELWIADAAAATARAITPPLLNAT
                     AGGACNWMPDGARLVCRLVPANRGPGPVAPPVPAGPTVQQTSGRAAPVRTYEDMIEDA
                     YGEALFDYYFTSQLALVDATSGATTAIGTPAVYDDAAPSPDGAYLFVSKTVRPYSYLV
                     PQEDFPKVYEVWSSRGEPVREIARIPLAETTPVGGVRTGPRLVTWRPDAPATLVWVEA
                     LDGGDTRRPADIRDRVVTLPAPFSAEPAELARTRDRFAGLRWGAGIALLSEANRVKRW
                     TRTWLLDTQHPGVAPRAVWDRSAEDRYGDPGFPVLRPVGTGAMLLQSGDFIYLIGAGA
                     TAQGDRPFLDRLNLRTLRAERLWRADSVHYETVVALLDDRAGRFVTRRESTREPPNYY
                     VREMRGHRLRALTAFPDPAPQLAGVKKQLVTYRREDGLPLSGTLYLPPEYRRGTRLPV
                     IMWAYPTEFATAAAAGQVTATTNRFTVYRGPTHLLLLTQGYAVFDDPKMPIVGGDTAN
                     NHYVEQLAMDARAAVRAVVDLGVADPDRIGVGGHSYGAFMTANLLAHTDLFRAGVARS
                     GAYNRTLTPFGFQSEERTFWEAPAVYQRMSPFVYADSINEPILLIHGEADNNSGTFPI
                     NSERLYAAIKGLGGTARLVMLPLEAHGYAARESVLDVLAEMVDWFDHYVKQAPPRPVV
                     GTR"
     gene            complement(9079..9987)
                     /locus_tag="AC16JC_17780"
     CDS             complement(9079..9987)
                     /locus_tag="AC16JC_17780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011525387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR5166608.1"
                     /translation="MSIRPIKRIVQATPTIEGAGVKLRRAFGFGDTGDYDPFLLLDDF
                     RNERPDDYLAGFPWHPHRGIETITYVLKGTVNHGDSLGNSGTLGAGDVQWMTAGSGIL
                     HQEMPQGDPQGKMHGFQLWANLPASLKMTAPRYQDIAARDIPEVTDDDGTVVRVVCGD
                     FWGKAGPVKGVAADPRYLDVWVPPGKRKTLAVETGRHAFAYVFDGSGTFRDASQPFGV
                     LTERVGTADVAPVREETGNRSLVLFDRGDEVTVQAGDQGIRFLLVSGKPIEEPVAWYG
                     PIVMNTREELQQAYAELRNGTFIKQR"
BASE COUNT         1547 a         3548 c         3559 g         1477 t
ORIGIN      
        1 cgggcggccc gatccggcgc cccttcgccg tgacgccggg ccgcgacggc gatcaggccg
       61 acctcgacca tttccccgag attcagcagg atccagatgt agaagccggc ggtggacacg
      121 gcggcgagtg ccgccgggcc gatgagccgg ccgacccaca ccgcgtcgat caccccgaac
      181 gtgaagtggc acgccatcat cgcgacggcg gggagggcga gacgcaggat gaccggccgg
      241 agcgggccga tggtgagcac ccccggggtc ccgggggggg cggcggcggt gctcacggcg
      301 ggcggtgcgc catctcgtgc agaaaccgct ggaacaccgg attcgccggg taccgccccg
      361 ccaatccatt tacgagcccg accgcttcgg cgcgccggcc ggcgcccgtc cgctcattcc
      421 ggttcgcctc gcgcaccagg gctgccgcga gtacccagct cccctcgaca cgcgccagat
      481 ctccggactg ggccacgcgc cgcatcatcg ccagaccctc atcggcattg ccgccaccga
      541 gcccgatgat ccggcccacg attttcgcga gtgtgctggc ccgcgcgaga ccgtagctgt
      601 agaggcccag cccgaggaag cagtcggtgc aggacgagtc ggcggccagg acgcgacggt
      661 aggcatcgcg catcgccttc gcgtccctgg cggcgcccca gttgtgcccg tggagctcgc
      721 gctcgcgggc acgatagccg agagcggtgc cgagccagaa gtcccgctcg atgccgggcg
      781 cgcggacctt cccgagctcg atggcgcgat cgagcagcga gtcgatgcgg ctcgcggcga
      841 agtcgtcgga atccttggcg gcggcccacc agatataggc gcccgcttcg aaggtgagcg
      901 gcgcagggtc ggcggtgtcc cgggcggcca ggtcggcgaa gtatcgggcg gccgcggcga
      961 aatacccggc atagagcgta tcgagacccg cccggagctc ggcgcgtctg cctccgtcgg
     1021 cggcctgcag cagcaacagc gcggccgtga ggagccgcat ttcagcgcgt aacggggaga
     1081 ggcccggaag gcgcggactg gagcagcgcc gccacctggc gggccgtgtg ggccagggcc
     1141 ttgccggccg gcgagtcggg ctgggagacg acgatcggct gcccgccgtc ggccaggcga
     1201 gcgagaccgg cctgcagagg aatctgcccg agcagcggaa cgccgagctc ggcggcgagc
     1261 cgttcaccgc cgcccgacag gaacagctcg ctccgctcgc cgcagtgctc gcacacgtag
     1321 tagctcatgt tctcgaccac gcccagcacc ttcgtcccca cccgctcgaa catcttggcg
     1381 ccccgcagcg agtcgccggt cgcgacctcc tgcggcgtcg tcacgatgag agcaccgtgc
     1441 aggtgaatcg tctgcaccag ggagagctgg gcgtcaccgg tgccgggggg cagatccacc
     1501 aggaggaagt ccagatcgcc ccagtccacg tcgtgcagga actggtggat gatcttcatg
     1561 atgatcgggc cgcgccagat ggcgggctga tcgcgctcca cgatgaagcc cagcgacatg
     1621 agcttgacgc cgtgcgcctc gagcggctgg atcttgccgc cctgcacggg gggcttttcg
     1681 tccacgccca tcatgcgggg aatgttgggt ccgtagatgt cggcatccat caggccgact
     1741 gcatgaccgg cccgcgcgag cgccacggcg aggttgaccg agacggtgga cttgccgact
     1801 ccccccttcc ccgacgagat ggcgagcgca cggcccaggc gaggcatgtc ttccggggac
     1861 gggggaggag ggacggggga cgaggccctg cccggccccg tcccggaacg tgacgcgggt
     1921 ccggccggcg ttgcagaatc cacgacgctg atcttcacgc ccgtcacgcc ctccagcgcc
     1981 tgcacggccc ggcgcgcgtc gcgcaccagc gtggcgggat cctcccgttt cagatgaaac
     2041 gtgaaggaca cctggccggc gtcgtcgacg gcgagatcgt gaatgcggtc gggctgggcg
     2101 agtgcggcga gtgccgcgga gacgcgctgc tggatttcgt tgggcacgac gggaagataa
     2161 tagctccgtc agtgccgcag gccggccacc acggcgaggg gcacgagcac gtagagcacc
     2221 aggtcgcgca cgaggtagac gagcacgacg accgcgacga acgcccagcc gcgccgcttc
     2281 agcaggcctt tccagccgag ttgccggcgc tcgtcgcgca gggtccggta gaagcccagg
     2341 agccccatca cggaaagctt gagcccgggg ggccgttgtc gcaaggcagg gcggcagacc
     2401 gcgcctgcag cacgcgcctg cgctccttcg tgtagtgcca ccagggcctc gccggtgctc
     2461 cttccatgaa gcgcacggcg gagatgaaga catccagcac gcacgggtcc tggcgctcgc
     2521 ccgtggcggc gcacagcgcg tcatacagcg cgtaggggtc ccgcccccgc aggtcggcgg
     2581 gaaaccgcac gcccaccagg cgcagatccg ctgcgagcga cggcccgacg ttgcggatct
     2641 gctcgaggcg cgtcagcgat tcgcgcgccg ccctcacgcc cctgcctcct cggagaactg
     2701 cgcgaatccc acggtgttgc ctccgggctc gcgcacgtac agctcggtac tgccatagaa
     2761 ggtcttgtgg cggggcttca cgatcggggc gccggcgacc gcgcgctcta tagcgtcgag
     2821 atccggcacc gtgatgaaga gcacggtgga atgtcccacc agctcgctcg cggaccccgg
     2881 actgtcggcc agcacgctcg cacgggtctg gtacatgact tcgatcccgt ccttctccac
     2941 gctggcgaag acgagcttgc cgtccggccc ggggaccttg ttcttggctt cgaagccgaa
     3001 ccggcccacc cagaaggcga gacacggctc cacggagtcg acgatgagga cgggggtaag
     3061 gcgcgtgagt cggatggcgg cggccatggc aaggctcctc tcggtctggg acctcaagtt
     3121 tagccgtcgc cagggccgcc gtattggacg aatccgacag gccgggcgtc tcctcccggg
     3181 tctactgccc gacggtgaag tcgatgcgct tcagtacggc gcctccggca tcccggatct
     3241 cgacgtgcca gggcccggtc cactccgccg ggacggtctt gcggctccag gtgcgccacg
     3301 gcgaaccgcg cgcggccagc tcgacgtcgg cgacctgctg atccccgtga aaccacacgt
     3361 ggtgaatcgt gcccggcgcg tccgccgagg ccccctcgat ctgcgtgaag cacacgagcc
     3421 cgccgacgtc cgcggggaag gccgcgcccg tgtcctgcgg cgcgcggtcg gccaccgagc
     3481 gcgccacgac gcccacggcg accgacagcg ccccggcggc gggagccgcg ggagccgcgg
     3541 ctgccttggt ggaatcctgc gcaaccagcg ggccggctac ggctgcgaac acgagcaggc
     3601 tgagcatgct gttcatgacg ttcccgtaga atgggttcgc ccgttgtacg gtggcggatc
     3661 gccccggttc cggcgaaaac gggtagccgg aggacgagtg accaggatca ccccttgcac
     3721 gccggcgccc ggaacgggtt tgcgtccgcg gcggccaccg gcgcgggctg tcgcggccac
     3781 gtgttgtcgt ccacgttcct gtcggggaac cggcagtgcg gatcgagcac gacgcgcgcg
     3841 atcttgcgcg ccccgaagtc gagtctcgcc tcgaaggtcc ggctcccgcc gaaccacgcg
     3901 tccaccggcc aggtcacgat cgccgtggaa tcgtctacga gggtggcgtt cgtcatcttc
     3961 ttcggcgcgg gtcctcccgg ggcgaaatgc acggccagca cgacgggcga gggcatctgt
     4021 ccgtcctggc gcacggtgac cgtggtgcgg gagccggccg tgcggacctc ggcgatcgac
     4081 ccatccaccg catcggtcgt gaagagccac gagtaccaga accacccgag ctcctggtgc
     4141 agcgcgttgc tcatgaagaa cgcgtagtcc cacggtgacg ggtgcttgaa ccgccacgtc
     4201 ccggcatact cgcgcatcgc gcgccacacc gcgctgtcgc cccccgcgac gacgccgccg
     4261 agcatcgaga gcatcatcgg ggccttgagg tacgcctgga agccgtacat cgggccgccg
     4321 tagttggcgt cccacatcag cggggcttcg cgctcgttcc cgctgacggc gccgtacgcc
     4381 tgccccacac cgtcgaggtt cggaggctga tgctgccggt ccgcgcgcga gaggatgttc
     4441 atgtactggt tgaagccctc gtccatgaac ccgtaccagg tctcgttgac gcccagcatc
     4501 atcggccacc actggtggcc ggtttcgtga tcggaggcac cggtgccgga catgatgaac
     4561 atcgggtact ccatgccgcc ctccggcccg tccaccatgg tgaggatcgg gaaggcgtac
     4621 ggaatccaca gccgcgaata gaactcgagc gcgtgtcgca cggtcggacc cgcgtcggcg
     4681 tactgccgtt cgtgtcccgg cagatacatg atgtcgatgg ggatagcgcc cttccccggg
     4741 atgtcggcgt gggtcgcgtc ccacacgaac tgattcgagg cggcccaggc gacgtcgccg
     4801 gccgtgtcgg ccacgaaatg ccagaccagg cgatcgccgg ccgcggtcgc cttgcccggc
     4861 cccagttcat cgggacccga gatccgtacc gtcgagtcgg cggcgaggac atgagtcagc
     4921 cgttcccgtt cgaccgcgga gagcacctcc gcggggttct gcagcagcac cgtggcaccc
     4981 accagccagc cggcggggag gtcgagcttc acatcgaacc ggccgaagtt gttgtagaac
     5041 tccgagggcc cgaggtaggg atcggtgtcc cacccgcccg ggcgcaggtc gtcgaacacc
     5101 gccacgcgcg gataccactg ggcgacctga tagagcgaat cgccccaggc gcccatccgg
     5161 acgccgcgcg tctgtggcgc gactttcggc accgtgaact gccagtccgc ctcgagcgtg
     5221 acggaaccgt gcgcggggac cggattcggc agcgtcacgt tcgccaccgt ctgcgccgtg
     5281 tcgtacgcgg cgaggatgac cggcggcggc tctccgttcg gcccgcgccg gaacacccgc
     5341 ggtggaggcg tgaggttgac cgcgttgccg tcgacactca gcgccgtcag ctgcatgccg
     5401 cccgtgatct gggtgacgac ttcggcgcgc ggcacgttgg cggcgaacag attctggtcg
     5461 agtcgcagca cgatccggct catgacggtg tcgccggcgt tctgcaccag cacccgctcg
     5521 tgccccgtca gcacaccggt cgcagggtcg aagcgggcgc tgatcgtgta gtcggtccag
     5581 agctgccagt agttccggcc ggggcgcccg gtggaatccc gggttcccgc ggccagggcg
     5641 tggcggatca tgcccgtcat cgggatgtcg cgtcgcacgg cgcggtcggg atacctcgct
     5701 gcggtctggg accgaccggt ggaaggaacc atgccgagca tgccgcacag ggtgagggta
     5761 cacgcaacgc gcgtctgcat tgcatctcct tgctgggagg accgacggct atgcttcgtt
     5821 cgtcaacaat agtgcgaccc ggatcaggat ggtacgatgc gactggccac gatgttggct
     5881 gtgggctccc tcgccgcttg cggcacgccc gcgcgtcccg cgccggccac ggcgccgacg
     5941 cccgccaggg cgcccgctcc gcttcccgcg cccctgcgct ggatgcgccg ctccgccgag
     6001 taccgggcac tcacccggca gatctacgcc tttgcggccc tgcggctcga cgagctggcc
     6061 cggggacgcc cggcggggag ctgggccgtc atcctcgacg cggacgagac cgtgctcgac
     6121 aacaccgagt acgagcgccg ccgcgcgagc gtggatagcg gctacagcga cgcgagctgg
     6181 gcgtcgtggg tgcgggagcg cgccgcggga gcgattcccg gcgccgcggc attcacggcc
     6241 acggtccggg gtctcggtgg acgcgtcgtg atcgtcacca atcgcgccga ttccctgtgc
     6301 gcggacacgc gcgccaatct ggtgaccgcc ggcgtcgcga ccgacctggt gctgtgcatg
     6361 cccccggggg aatcggacaa gaatccgcgc ttcgagcgcg tacagcaggg cacggccgcg
     6421 ccgggcgtag cggcgctgac ggtggtcgaa tggatcggcg acaacatcca ggacttcccg
     6481 cacctgaccc aggacgtccg cagcgattcg acggcgctcg ccgcattcgg ccgccggttc
     6541 ttcgtgctgc ccaacccggt gtacggctcc tgggagcgga accaggagcc gtgagtcggc
     6601 tcagcgtgtg cccaccaccg ggcgcggcgg cgcctgcttc acgtagtggt cgaaccagtc
     6661 caccatttcg gccagcacat cgagcaccga ctcccgcgcc gcgtagccgt gcgcctcgag
     6721 cggcagcatc accagccgcg ccgtccctcc cagccccttg atggcagcgt agagccgctc
     6781 cgagttgatg gggaacgtcc ccgagttgtt gtccgcctcg ccgtggatca gcaggatcgg
     6841 ctcgttgatg ctgtcggcgt agacgaacgg cgacatgcgc tggtacaccg cgggcgcctc
     6901 ccagaaagtg cgctcctcgg actggaagcc gaacggcgtg agcgtccggt tgtacgctcc
     6961 cgagcgcgcc acgccggcgc ggaacaggtc ggtgtgggcc agcagattgg cggtcatgaa
     7021 ggcgccgtag ctgtggccgc ccacgccgat ccggtcggga tcggcaacac ccagatccac
     7081 caccgcgcga accgccgcgc gcgcgtccat ggcgagctgc tccacgtagt ggttgttggc
     7141 cgtgtcgccg cccacgatcg gcattttggg gtcgtcgaac acggcgtagc cctgcgtcag
     7201 cagcagcagg tgcgtcggtc cacgatagac ggtgaaccgg ttggtggtgg cggtcacctg
     7261 ccccgcggct gcggccgtgg cgaactccgt cggataggcc cacatgatca ccggcaacct
     7321 ggtgccgcgg cggtactcgg gcggcaggta gagcgtcccc gacaggggga gtccgtcctc
     7381 gcgccggtag gtgaccagct gcttcttcac accggcgagc tgtggggcgg ggtcggggaa
     7441 tgctgtcagg gcgcgcagcc ggtggccgcg catctcgcgc acgtagtagt tgggcggctc
     7501 gcgcgtcgac tcgcgtcggg tgacgaaccg gccggcgcgg tcgtcgagca gcgcaacgac
     7561 agtctcgtaa tggacggagt cggcgcgcca cagccgctcg gcgcgtagcg tgcgcaggtt
     7621 caggcggtcg aggaacggtc ggtcgccctg cgcggtcgcc cccgcgccga tcaggtagat
     7681 gaaatccccg gactgcagca gcatggcgcc cgtccccacg ggtcgcagaa ccggaaatcc
     7741 gggatcgcca tagcgatctt ccgcgctgcg gtcccacacg gcgcggggtg cgacgcccgg
     7801 gtgctgcgta tccagcagcc acgtgcgcgt ccaccgcttc acccggttgg cctccgacag
     7861 caacgcgatg ccggcgcccc agcgcagtcc ggcgaagcgg tcgcgggtcc gcgccagctc
     7921 ggcgggctcc gcgctgaacg gggccggcaa cgtcaccaca cggtcccgga tgtcggcggg
     7981 ccggcgggta tcgccaccat ccagcgcctc gacccagacc agcgtggccg gcgcgtcggg
     8041 gcgccaggtg acgagccgcg gcccggtgcg cacgccgccg accggcgtcg tctcggcgag
     8101 cggaatacgc gcgatctcgc gcaccggctc gccgcgggac gaccagacct cgtacacctt
     8161 ggggaagtcc tcctgcggca ccaggtagga gtaaggccgc accgtcttgg agacgaagag
     8221 ataggcgccg tcgggcgacg gcgcggcgtc gtcatagacg gcgggcgtcc cgatcgcggt
     8281 cgtcgcgccg gacgtcgcgt ccaccagggc caactgcgaa gtgaagtagt agtcgaacag
     8341 cgcctcgccg tacgcgtcct cgatcatgtc ctcgtaggtg cgtaccggcg cggcgcgacc
     8401 gctggtctgt tgcacagtcg ggccggcggg aacggggggc gcgacgggcc ccggtccgcg
     8461 attcgccggc acgaggcggc acacgagccg cgcgccgtcc ggcatccagt tgcacgcgcc
     8521 cccggcggtg gcgttcaata gcggcggcgt gatcgccctg gccgtcgccg cggccgcgtc
     8581 ggcgatccac agctcgatgc cgtccgggcg tgtgttcgtg aaggcgaagt ggcgcgcgtc
     8641 cggcgcccag tccacgtctc ccaccacggc ccccgcgggc agcgggatac ggcgctcgag
     8701 cccatcggcc acgcgcttca acaccaggcc ggtgatccgc tggccccgtg ccggaccgtt
     8761 ggtgcgcgga tcgatccgcc aacccgcgag ccgcagcatc ggcgccgcga attcggcgat
     8821 ggtgggcatg ctcggctgct ccagcagcag gagccactgg cggtcgggac tcaaggtggc
     8881 cgcgggcaac ggcggcgcgt cgaggatgtc gacgatggcc tgcggtggag tgcggtagcg
     8941 ctcctgagcg ccgatcggcg ccggcaacag gagcagcagc agggcccaac ggaaggtgtg
     9001 tcgcatggaa cccccgcgaa tcgggtgagc gcgtgatcac cctatggtat gcgggggtcc
     9061 cgccggccga tagccgcctc agcgctgctt gatgaacgtc ccgttccgca gctcggcata
     9121 ggcctgctgc agctcttccc gtgtgttcat gacgatcggt ccgtaccacg cgaccggctc
     9181 ctcgatcggc ttgcccgaga cgagcaggaa ccggatcccc tggtcgcccg cctgcaccgt
     9241 gacttcgtcg ccgcggtcga acagcacgag ggagcggttg cccgtttctt cccggaccgg
     9301 agcgacatcc gccgttccga cgcgctccgt gaggacgccg aagggctgcg acgcgtcgcg
     9361 gaaggtgccg ctgccgtcga acacgtaggc gaaggcatgg cgacccgtct cgacggcgag
     9421 cgtcttgcgc ttccccggcg gcacccagac atcgagatag cgggggtccg ccgcgacgcc
     9481 cttcaccggg ccggccttcc cccagaaatc accgcacacc acgcgcacca cggtgccgtc
     9541 gtcgtcggtc acttcgggaa tgtcgcgggc cgcgatatcc tggtagcgcg gcgcggtcat
     9601 cttgagcgac gcaggcagat tcgcccacag ctggaagccg tgcatcttcc cctgcgggtc
     9661 gccctgcggc atctcctgat ggaggattcc gctgcccgcc gtcatccact gcacgtcgcc
     9721 ggcgccgagc gtgccggagt tgcccaggct gtccccatga ttgaccgtcc ccttgagcac
     9781 gtaggtgatg gtctcgatgc cacgatgcgg atgccacggg aacccggcga ggtagtcgtc
     9841 gggacgctcg ttccggaaat cgtcgagcag gaggaacggg tcgtagtcac ccgtgtcccc
     9901 gaacccgaag gcgcgccgca acttcacgcc ggccccttcg atggtggggg tcgcctgcac
     9961 gatgcgcttg atcggtcgaa tcgacacagc tgctccttgc gggaacccgg acataacacg
    10021 ccgccgggcg ccggtgttcc ggttgccgtt ccggcggcgg cgggcagatt gtcccgccat
    10081 ggccgatatc tatcacgatt tcccgatcaa ggcaccgccc gaacgcgtgt a
//
LOCUS       DBNGZW010000760         3268 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1764321, whole genome shotgun sequence.
ACCESSION   DBNGZW010000760 DBNGZW010000000
VERSION     DBNGZW010000760.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3268)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3268)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3268
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1764321"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3107
                     /locus_tag="AC16JC_17785"
     CDS             <1..3107
                     /locus_tag="AC16JC_17785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011684403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="WD40/YVTN/BNR-like repeat-containing protein"
                     /protein_id="HIR5166609.1"
                     /translation="APPARAPAAARAPARAPAPPAAAPVVVDSETFAGLGARNIGSAA
                     MSGRIAALAAVHEGDRLTLYVGAASGGVWKSQNGGTTFKPVFDKEPVQSIGAITIDPT
                     NPKIVWVGTGESWVRNSVSIGDGVYKSTDGGETWTNMGLRESERISQILVDSANPNTV
                     YVCVPGKLWSDSDERGVYKTTDGGKTWTKVLAGANPSTGCSMMAMDPTAPGTLLAGMW
                     DFRRKGWTFRSGGDGPTAPSASGLFRSTDGGATWTELTGELAKGLPPKPWGRVAVAIA
                     PSKPNVIYATIEAVQPKDGLYRSDDGGRTWQLRDRSQMMIWRPFYFASLTVDPRDENR
                     VYKPDGGLIVSTDGGKSFSGISGGAHGDFHVVWVDPRNTDHLITGDDGGLWYSDNAGT
                     HWWKADNLPVSQFYHVSVDSDRPYNVYGGLQDNSSWVGPSQYPGGITSSRWENMYGGD
                     GFWMFVDPTDPTYVYAEYQGGSIARINRTTHELRDIKPLPRYAEGKLRFNWNAPIHLS
                     ASHPGTIYIGAQFLFRSRDYGQTWERISPDLTTNDPEKQQQEQSGGVTVDNSYAEMHT
                     TIYAIAESPRSDQVIWVGTDDGNVQVTQDGGKTWTNVVGNVPGVPKNSWVSSIDAGRF
                     DAGQAFVTFDRHTFGDMRPYAYRTRDYGRTWTPLIAADAPVRGYAHVVKQDPADPDLL
                     FLGTEFGLWISVDAGGHWARFKGGDMPSVAVRDLAIHPRDDDLVIATHGRGIWIIDDI
                     TPLRQLTPQALAQPVAFFQARPVVQALPASGGWANGDAAFVGPNPPGDAVITYSLRRR
                     HVFGDMTLEVFDSAGTSLGPIPTSKRKGLSRITWGMRLPPPRVPPAASAAGGAIVGPR
                     FLPGSYAVKLTENGVVYQTTLTVVPDPRSTHTMPERVAQYRLSLQLADLLNRMTDDVL
                     RINAMRRSLEARAAGLPAGDSLAATLRRAAGTVDSLRKKIVATTEGGAITGEERLREN
                     LADLYGSVVNYEGRPSQTQVERADAIARELADVMQAFDAWAARVVPGLNAQLASRHLE
                     PLPR"
BASE COUNT          527 a         1133 c         1161 g          447 t
ORIGIN      
        1 cggcgccgcc cgcgcgggcc ccggccgcgg cgcgcgcccc ggcacgggcc cccgcccccc
       61 ctgcggcggc gcccgtggtg gtggactcgg agacgttcgc gggactgggc gcgcgcaaca
      121 tcggctccgc ggccatgagc ggacgcatcg cggccctggc ggcggtgcac gaaggcgacc
      181 gcctgacgct ctacgtgggc gcggcgagcg gcggcgtatg gaagtcccag aacggcggga
      241 ccaccttcaa gccggtgttc gacaaggaac cggtgcagtc gatcggcgcg atcaccatcg
      301 accccacgaa tccgaaaatc gtctgggtgg gcaccggtga gtcgtgggtg cgcaactccg
      361 tgtccatcgg cgacggcgtc tacaagtcca ccgacggcgg cgaaacctgg accaacatgg
      421 ggctccggga atccgagcgc atctcccaga tcctggtgga ttccgcgaac cccaacaccg
      481 tgtacgtctg cgtccccggg aagctgtgga gcgacagcga cgagcgcggc gtctacaaga
      541 cgaccgacgg cgggaagacc tggacaaagg tcctggccgg ggccaatcca tccaccggct
      601 gctcgatgat ggcgatggat cccacggccc ccggcacgct gctcgcgggg atgtgggact
      661 tccgccgcaa gggctggacg ttccgctccg gcggcgacgg gccgacggcg ccctcggcga
      721 gcgggctgtt ccggagcacc gacggcgggg ccacctggac ggagttgacc ggcgagctcg
      781 ccaaggggct gccgcccaag ccgtggggtc gcgtggcggt ggccatcgcc ccctcgaagc
      841 ccaacgtgat ctacgcgacg atcgaggccg tgcagcccaa agacggcctg tatcgctccg
      901 acgacggcgg gcgtacctgg cagctgcggg accgcagcca gatgatgatc tggcgcccgt
      961 tctacttcgc cagcctcacg gtggatccca gggacgagaa ccgcgtctac aagccggacg
     1021 gcggcttgat cgtcagcacc gacgggggca agagtttcag cggcatcagc ggcggggcgc
     1081 acggcgactt ccacgtggtg tgggtcgatc cccggaacac cgatcacctc atcaccggcg
     1141 acgacggcgg cctgtggtac tccgacaacg ccgggacgca ctggtggaag gcggacaacc
     1201 tgccggtaag ccagttctac cacgtcagcg tggacagcga ccggccctac aacgtgtacg
     1261 gcgggttgca ggacaacagc tcctgggtgg ggccgtcgca gtatcccggc gggatcacga
     1321 gcagccggtg ggagaacatg tacggcggcg acgggttctg gatgttcgtg gatcctaccg
     1381 atccgaccta cgtgtacgcc gagtaccagg gcggctcgat cgcccggatc aaccgcacga
     1441 cgcacgagct gcgcgacatc aagccgttgc cgcgctacgc cgagggcaag ctgcgcttca
     1501 actggaatgc gccgattcac ctgagcgcga gtcatccggg gacgatctat atcggcgcgc
     1561 agttcctgtt ccgctcccgc gactacggcc agacgtggga gcgcatctca ccggatctca
     1621 cgaccaacga tccggagaag cagcagcagg agcagtcggg cggggtgacg gtggacaact
     1681 cctacgccga aatgcacacg acgatctacg ccatcgccga gtcgccccgg agcgatcagg
     1741 tgatctgggt gggcaccgac gacggcaacg tgcaggtgac gcaggacggc ggcaagacct
     1801 ggaccaacgt cgtgggcaac gtcccggggg tcccgaagaa ctcgtgggtg agctccatcg
     1861 acgccggccg gttcgacgcg ggccaggcgt tcgtgacctt cgaccggcat accttcggcg
     1921 acatgcggcc gtacgcctac cgcacgcggg actacggccg cacctggacg ccgctcatcg
     1981 cggcggacgc gccggtgcgc ggctacgcgc acgtcgtcaa gcaggatccg gccgatcccg
     2041 atctgctgtt cctggggacg gagttcgggc tgtggatctc ggtggacgcg ggggggcact
     2101 gggcgcgctt caagggcggc gacatgccgt cggtggcggt gcgcgacctg gcgatccacc
     2161 cgcgcgacga cgatctggtg atcgccacgc acgggcgcgg catctggatc atcgacgaca
     2221 tcacgccgct gcggcagctc acaccgcagg ccctggcgca gccggtggcc ttcttccagg
     2281 cgcggccggt ggtgcaggcg ctgccggcct cgggcggctg ggcgaacggc gacgcggcgt
     2341 tcgtggggcc caatccgccg ggcgacgcgg tgatcaccta ctcgctgcgt cggcgccacg
     2401 tgttcggcga catgacgctc gaggtgttcg actcggcggg cacgtcgctg ggccccatcc
     2461 ccaccagtaa gcggaagggg ttgagccgga tcacctgggg catgcggttg ccgccgccac
     2521 gcgttccgcc ggcggccagc gccgcgggcg gcgcgatcgt gggcccgcgg ttcctgcccg
     2581 gcagctacgc cgtcaagctg acggagaatg gcgtcgtcta ccagacgacg ctcaccgtgg
     2641 tgccggaccc gcgctcgacg cacaccatgc cggagcgcgt ggcacagtac cggctctcgc
     2701 tccagctggc cgatctcctg aaccgcatga ccgacgacgt gctgcgcatc aacgccatgc
     2761 gccgctcgct cgaggcgcgc gcggccgggc tccccgcggg cgattcgctc gccgcgacct
     2821 tgcggcgcgc ggcggggacg gtggattcgc tgcgcaagaa gatcgtcgcc accaccgagg
     2881 gcggcgcgat caccggcgag gagcggctgc gcgagaatct cgccgatctg tacggcagcg
     2941 tggtgaacta cgaggggcgc ccgtcgcaga cccaggtcga gcgcgccgac gccatcgcgc
     3001 gcgagctggc cgacgtgatg caggcgttcg atgcctgggc cgccagggtg gtccccgggc
     3061 tcaacgcgca gctcgcgtcg cgccacctgg agccgttgcc gcggtaactc ccctccgccc
     3121 tgcaaccccg ggtgtcaggt gcccggggtc cggggtcagg gaacaccgaa gacagtgtag
     3181 ggcctccacc gtcctgcacc gtcctccacc gtcccggggc gacacgctga agccctaaag
     3241 gacttcctcc ggtcgcgtga gccccgca
//
LOCUS       DBNGZW010000761         2136 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1082724, whole genome shotgun sequence.
ACCESSION   DBNGZW010000761 DBNGZW010000000
VERSION     DBNGZW010000761.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2136)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2136)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2136
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1082724"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /gene="pssA"
                     /locus_tag="AC16JC_17790"
     CDS             complement(<1..611)
                     /gene="pssA"
                     /locus_tag="AC16JC_17790"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HIR5166610.1"
                     /translation="MRRRHQSGDGARHPRRRRWQELREHPRRGIFLLPSLLTTANLFC
                     GFLAIVLSSQGRFVDAAVAVFVGMVLDILDGKVARLTRTTTQFGVEFDSLADVVSFCV
                     APAFMLYALVLAQAGRIAWLGAFLFVICGALRLARFNVHQGVTDRRYFVGLPTPAAAG
                     IVAATALLLEGVEIERWHAAAISAGTVVVALLMVSTCRYYSFK"
     gene            complement(608..1234)
                     /locus_tag="AC16JC_17795"
     CDS             complement(608..1234)
                     /locus_tag="AC16JC_17795"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004872382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HIR5166611.1"
                     /translation="MRIPVAAEGWPFISPPAAAACILAWMGWWPAAAALGAVALCCLG
                     FFRDPDRTAPAVPGAVLAPADGRVMRIVEIEDPWVGRAVRVSIFLSPLDVHVNRSPVA
                     ALVEGVQHAAGRFLAAYRDEASELNERCTLTLQGEAARVSVKQIAGVVARRIVCRVRQ
                     GDKLQAGERYGLIRFGSRTDLLVPKGTEIRVRVGDRVRGGETIMGVVR"
     gene            complement(1252..>2136)
                     /gene="ilvC"
                     /locus_tag="AC16JC_17800"
     CDS             complement(1252..>2136)
                     /gene="ilvC"
                     /locus_tag="AC16JC_17800"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015743924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR5166612.1"
                     /translation="GQDVVVGLYKGSKSWTKAEKDGLKVMTVNDAAAAADIIMILLPD
                     QTQRQVYEESIKGVLGKGKSLMFAHGFNIHFNQVVPSPDVDVSMIAPKAPGHVMRDLF
                     TQGPGVPALVAVYQDVSGKARDVALAYGKGVGCTRAGVIETTFKEETETDLFGEQTTL
                     CGGVSHLIKSAFETLVEAGYQPEVAYFECMHEMKLIVDLFYQGGLAYMRYSVSDTAEY
                     GDYTRGPRVVSPETKAEMKRILGEIQSGQFAREWILENQAHRAGFLAMRKREAEHPIE
                     EVGARLRKMMSWIKPPRM"
BASE COUNT          345 a          775 c          703 g          313 t
ORIGIN      
        1 tccttgaaac tgtaatagcg gcacgtcgag accatgagga gcgccaccac gaccgttccc
       61 gcgctgatgg ccgcagcgtg ccagcgctcg atttcgacac cctcgagcag gagcgcggtc
      121 gcggcgacga tcccggcggc ggccggcgtc ggcagtccca cgaaataacg gcggtccgtg
      181 acgccctggt gaacgttgaa gcgggcgagc ctgagcgcgc cgcagatcac gaagaggaag
      241 gcgccgagcc aggcgatgcg gcccgcctgg gcgagcacca gcgcgtagag catgaaggcc
      301 ggggcgacgc agaacgacac cacgtcggcc agcgagtcga actccacgcc gaactgggtc
      361 gtggtccggg tcagccgggc gactttgccg tcgaggatgt ccagcaccat gccgacgaag
      421 acggcgaccg cggcgtcgac gaagcggccc tgggaggaga gcacgatggc gaggaagccg
      481 cagaagaggt tggcggtcgt gagcaggctc gggagcagga agatgccgcg gcgcgggtgc
      541 tcgcgcagct cctgccagcg ccggcggcgc ggatgccgcg ccccgtctcc gctctgatgc
      601 cggcgtctca cctcaccacc cccatgatcg tctcgccccc tctgactctg tcacccaccc
      661 gcactctgat ctccgtcccc ttcggcacga gcaggtccgt ccgcgagccg aaccggatca
      721 gcccgtaccg ctccccggcc tggagcttgt ccccctgcct cacccggcac acgatgcgcc
      781 gcgccaccac accggcgatc tgtttcacgc tgacccgcgc ggcctcgccc tggagcgtga
      841 gcgtgcagcg ctcgttcagc tccgaggcct cgtcccggta cgcggccagg aagcgccccg
      901 cggcgtgctg gacgccctct accagggccg cgaccggcga ccggttgacg tgcacgtcga
      961 gcggcgacag gaatatggac acccgcaccg cccgccccac ccacggatcc tcgatctcca
     1021 cgatccgcat cacgcggcca tccgccggag ccaggaccgc gcccggaacg gccggcgcgg
     1081 tccggtcggg gtcgcggaag aagcccaggc aacagagggc caccgccccc agggccgccg
     1141 ccgccggcca ccagcccatc caggccagaa tacacgccgc cgccgcgggt ggggagataa
     1201 agggccaccc ttcggcagcg acgggaatgc gcatgcgatc ccctgccgcc gctacatccg
     1261 cggcggcttg atccaggaca tcatcttgcg gagccgcgcc ccgacctcct cgatcgggtg
     1321 ctcggcctcg cgcttgcgca tggcgaggaa cccggcgcgg tgggcttggt tctccaggat
     1381 ccactcgcgg gcgaactggc cggactggat ctcgcccagg atgcgcttca tctccgcctt
     1441 cgtctcggga ctgacgacgc gcggaccgcg cgtgtagtcg ccgtactctg ccgtgtcgga
     1501 gacggagtac cgcatgtagg cgaggccgcc ctggtagaag aggtccacga tcagcttcat
     1561 ctcgtgcatg cactcgaagt acgcgacctc cggctggtag cccgcctcga ccagagtctc
     1621 gaaggcgctc ttgatcaggt gcgagacccc gccgcacagc gtcgtctgct cgccgaaaag
     1681 gtccgtctcc gtctcttcct tgaacgtggt ctcgatgacg cccgcgcgtg tgcagccgac
     1741 gcccttgccg taggccagcg cgacgtcccg cgctttcccc gacacgtcct ggtaaaccgc
     1801 gacgagcgcc ggcacgcccg ggccctgggt gaagaggtcg cgcatgacgt ggccgggagc
     1861 cttgggagcg atcatcgaga cgtccacgtc gggcgagggc acgacctggt tgaagtggat
     1921 gttgaacccg tgggcgaaca tgagcgactt gcccttgccg agcacgccct tgatcgactc
     1981 ctcgtagacc tgccgctggg tctggtcggg caggaggatc atgatgatgt cggccgcggc
     2041 ggccgcgtcg ttgacagtca tgaccttgag cccgtccttt tccgccttcg tccagctctt
     2101 cgagcccttg tacagcccca cgacgacgtc ctgccc
//
LOCUS       DBNGZW010000762         2653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_744877, whole genome shotgun sequence.
ACCESSION   DBNGZW010000762 DBNGZW010000000
VERSION     DBNGZW010000762.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_744877"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="AC16JC_17805"
     CDS             <1..897
                     /locus_tag="AC16JC_17805"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5166613.1"
                     /translation="GPGELRVGVARGAGDSAAASVLEGAPGLAAVVLDSVAARAALRT
                     GRVALVVVPGDPMVYRYDSTRTESRLARLEVDEALQRERGRADPVLVSDDRTTEPGSR
                     YIDFLIPGLLGMNLMGSGLWGVGFTVVQARTKGLLKRFLATPMRKGEYLLSYMLSRLL
                     FLVIEVAALIGFGWLVFDVGVRGSIAALGLVTLIGAFSFAGLGLLVASRAKTVEAVSG
                     LMNLVMLPMWILSGTFFSYARFPEALQPFVKALPLTGLNDALRGVMIDGASLAGVAAP
                     VGVVALWGALSFVVAVRIFRWR"
     gene            complement(899..1300)
                     /locus_tag="AC16JC_17810"
     CDS             complement(899..1300)
                     /locus_tag="AC16JC_17810"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR5166614.1"
                     /translation="MRRNSLAFVLAVAVTACSGSTVTGVNGGGGGGGTGGGTSASVTA
                     RDYAFSPATLTIKAGSSVTWTNDGPSSHTVTSDSTGVFDSGMLSPPMASMDPYGGASS
                     GQTFTMAFQTPGTYKYHCTNHATMRGTVVVQ"
     gene            1386..1805
                     /locus_tag="AC16JC_17815"
     CDS             1386..1805
                     /locus_tag="AC16JC_17815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166615.1"
                     /translation="MPDSISLKDYYYTTVPDKPGEGVRVLQALRDAGVNLLGYSGFPE
                     ARRAQLDFVPEDPAAFIAAAKAAKIKLTGPKKVFLISGEDRPGAVAGICEKLAGAKIN
                     VTTADAVCAGMGRYGAILWVKPRDVKKAAKVLGVNSP"
     gene            complement(1792..>2653)
                     /locus_tag="AC16JC_17820"
     CDS             complement(1792..>2653)
                     /locus_tag="AC16JC_17820"
                     /EC_number="2.7.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012298.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HIR5166616.1"
                     /translation="ERRLRRAFGDDYELLEEIGAGGFGRVYRVRDLGLEREVAIKVLH
                     PQLTADPGVMERFRREAMLAARLRHPNIVSIYDIQSRAGLQWYVMELVPGANLAQIVK
                     KDGPFPLQKTTRVLFEALSALEHAHALGLVHRDIKPENMLLEPTGLLRITDFGLALAL
                     RGDRFGGATSRSGTPQFAAPEQLLGERVDPRTDLYSLGAVAYFALLGRAPFEGPTPEA
                     ILAMQTTDNIPQLREARPDVSREIEDVLRRAVRGDPAQRYVNAAAFREALDLATGGVL
                     KKMLALLRVS"
BASE COUNT          368 a          875 c          962 g          448 t
ORIGIN      
        1 ggcccgggcg agctgcgcgt cggcgtggcg cgcggggcgg gggacagtgc ggcggcgagc
       61 gtgctcgagg gcgcgcccgg tctcgccgcc gtggtgctgg acagcgtggc ggctcgagcc
      121 gcgttgcgca ccggccgggt cgcgctcgtc gtggtgccgg gcgacccgat ggtgtaccgc
      181 tacgactcga cgcggaccga gagccgcctc gcgcgcctcg aggtggacga ggcgctgcag
      241 cgcgagcgcg ggcgcgccga ccccgttctg gtgagcgacg atcgcaccac cgagcccggg
      301 tcgcgctaca tcgactttct gatcccgggc ctgctcggca tgaacctgat gggcagcggc
      361 ttgtggggcg tgggattcac ggtggtacag gcgcggacca agggactgtt gaaacgcttt
      421 ctcgccaccc cgatgcgcaa gggagaatac ctgctgtcgt acatgctgtc gcgtctgttg
      481 tttctggtga tcgaggtggc ggccctgatc gggttcggct ggctggtgtt cgacgtgggc
      541 gtgcgggggt cgatcgcggc gttgggtctg gtgacgctga tcggcgcgtt ttcgttcgcc
      601 gggctggggt tgctggtcgc cagccgggcc aagacggtcg aggcggtgtc gggcctgatg
      661 aatctggtga tgctgccgat gtggatcctc tcggggacct tcttctccta tgcccggttt
      721 cccgaagcac tgcagccgtt cgtgaaggcg ttgcccctca ccggattgaa cgacgcgttg
      781 cgcggtgtga tgatcgatgg cgcctcactc gcaggcgtcg ctgcgccggt cggcgtcgtc
      841 gcgctgtggg gagcgctgag cttcgtcgtt gcggtgcgga tcttccgctg gcgctgacct
      901 attggacgac gacggtgccg cgcatcgtcg cgtgattggt gcagtggtac ttgtaggtgc
      961 cgggggtctg gaacgccatg gtgaaggtct ggcccgacga cgcaccgccg tagggatcca
     1021 tgctcgccat gggcggtgac agcatgccgc tgtcgaacac ccccgtcgaa tcgctcgtca
     1081 cggtatgact cgaggggcca tcgttcgtcc aggtcacgga gctgccggcc ttgatggtga
     1141 gggtcgccgg cgagaacgcg tagtcccgag cggtgaccga ggcgctggtg ccccctccgg
     1201 tccctccgcc gccgccgccg ccgttcacac ccgtcacggt tgatcccgaa caggccgtca
     1261 ccgccacagc cagcacgaac gccagggaat tgcgccgcat gggatgccct ccgttgcctt
     1321 cgggacgtcg tgaggcgccc gggcggattt atggtagctt ccgggatccc cgttccgagg
     1381 cgaccatgcc cgactccatc tccttgaagg actactacta cacgaccgtt cccgacaagc
     1441 cgggcgaggg cgtgcgggtg ctgcaggcgc tgcgggatgc cggggtcaat ctgctggggt
     1501 actcgggctt cccggaggcg cggcgcgccc agctcgactt tgtgccggaa gatccggcgg
     1561 cgttcatcgc cgcggccaag gcggcgaaga tcaagctcac cggtcccaag aaggtgttcc
     1621 tgatcagcgg cgaggaccgg ccgggcgcgg tggcggggat ttgcgagaag ctggcggggg
     1681 cgaagatcaa cgtcaccacg gccgacgcgg tgtgcgccgg gatgggccgg tatggggcga
     1741 tcctgtgggt gaagccgcga gacgtgaaga aggcggcgaa ggtgctgggc gtcaactcac
     1801 cctgagcagc gccagcatct tcttcagcac cccgccggtc gccagatcca gcgcctcccg
     1861 gaatgccgcc gcgttgacgt agcgctgcgc cggatcgccc cgcactgcgc gccgcagcac
     1921 atcttcgatc tcccgcgaca catccgggcg cgcctcccgc agttgcggga tattgtccgt
     1981 cgtctgcatc gccaggatgg cctcgggggt cggcccctcg aacggcgctc ggcccagcag
     2041 tgcgaaatag gcgaccgcgc cgagcgagta gaggtcggtg cgcgggtcca cccgctcccc
     2101 cagcagctgc tcgggcgcgg cgaactgcgg cgtgccgctg cgcgaggtcg cgccgccgaa
     2161 ccggtcgccg cgcaacgcca gcgccagccc gaaatcggtg atccgcaaca ggccggtcgg
     2221 ctcgagcagc atgttctcgg gcttgatgtc ccggtggacg aggcccaggg catgcgcgtg
     2281 ctcgagcgcg ctcagcgcct cgaacaggac ccgggtcgtc ttctggagcg ggaacggccc
     2341 atccttcttg acgatctggg ccaggttcgc gcccggcacc agctccatga cgtaccactg
     2401 caggccggcg cggctctgaa tgtcgtagat gctgacgatg ttgggatggc gcagcctggc
     2461 ggcgagcatg gcctcgcgcc ggaaccgctc catcacgccg ggatcggccg tcaactgggg
     2521 gtgcaacacc ttgatggcca cttcccgctc gagccccagg tcgcgcaccc ggtacacccg
     2581 gccgaatccg cccgcgccga tctcctccag cagctcgtag tcgtcgccga acgcccggcg
     2641 caggcggcgc tcg
//
LOCUS       DBNGZW010000763         4685 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1833222, whole genome shotgun sequence.
ACCESSION   DBNGZW010000763 DBNGZW010000000
VERSION     DBNGZW010000763.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4685)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4685)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4685
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1833222"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..764)
                     /locus_tag="AC16JC_17825"
     CDS             complement(<1..764)
                     /locus_tag="AC16JC_17825"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR5166617.1"
                     /translation="MAARRLRVLHVIWWGEVGGIALNLVDLARHAAAANHALTVCVLT
                     RSSPLIDALSDQNVRVAAMGARSGRDLLAWLRFTRFLRREAFDVVHDHTGTFLAAMAI
                     RLGAAKARTIHQEHGGINIPRLRARKRMFYRVFGRVFDRFIAVSGKIAEDMVAAGVER
                     GRIETITNPVDPDRFSPRQPRQEAKHALGIPETTVTVGTACRFAKEKDLDLFLDVARL
                     IHRQRHDVMFVMVGTGEEAEPLRRRAGEHGILPAVL"
     gene            836..1942
                     /gene="wecB"
                     /locus_tag="AC16JC_17830"
     CDS             836..1942
                     /gene="wecB"
                     /locus_tag="AC16JC_17830"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018506416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-hydrolyzing UDP-N-acetylglucosamine
                     2-epimerase"
                     /protein_id="HIR5166618.1"
                     /translation="MKTIAIVAGARPNFMKIAPLVRALRQDGGQLGFQIVHTGQHYDP
                     AMSEVFFEELAIPRPDVALGVGSGSLAQQTAKIMVGFERYCRAQAPHLVLVVGDVNST
                     LACSVVAKQQHIPVAHVEAGLRSGDMTMPEEINRIVTDAIADLLFVTEPSGVANLLRE
                     GKPREVVHHVGHVMVDNLLYQLDRLACVDTSGYVSSGIRARYPRYGAVTLHRPSNVDS
                     PEVFTRISSALREISHHLPLVFPVHPRTRVNLEKYGLDLGSRVMLTPPLSFMDFLNLW
                     TEATVVLTDSGGLQEETTALGIPCLTLRDNTERPITVDEGTNVVVGTDPARITDAVVT
                     VLRGEGKRGRRPELWDGHAAQRIAVVLRRALAAR"
     gene            complement(1948..3054)
                     /locus_tag="AC16JC_17835"
     CDS             complement(1948..3054)
                     /locus_tag="AC16JC_17835"
                     /EC_number="3.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012551.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0031176 - endo-1,4-beta-xylanase activity
                     [Evidence IEA];
                     GO_process: GO:0045493 - xylan catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HIR5166619.1"
                     /translation="MGPVLAHPACAAGTCARHSVLALTSILVWLGPGCTPAPTATDAT
                     IVEQSPPLRQLAAARHFVVGTAVDPNALRSDSLYRVKLATEYNGVTSEWAMKFGSLHP
                     GQQQYDFADADRLVAFAQANRMAIHGHTLVWGEALPAWITAGSFTKTQLLQVLRDHIS
                     TVVGHYRGRIATWDVVNEVVDGSAAPLRSTLWLQVIGPEYIDSAFVWARRADPAAALY
                     VNETHAEVVNPRSNALLALVQGLRARGIPVDGVGFQAHFVLPSPAAADLQANLGRFAQ
                     AGFDVRVSEMDVRIADSAAGTALTQEATAYRDMLDACLRVGPRCAGFTSWGFTDRYSW
                     IPKFYPGYGHALPLDADYRPKPAYTALADRLQQP"
     gene            complement(3169..3513)
                     /locus_tag="AC16JC_17840"
     CDS             complement(3169..3513)
                     /locus_tag="AC16JC_17840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166620.1"
                     /translation="MTTPAANPALGEVRSAILTMARRIEQTADLPQEGIAELKNAVDD
                     VRIRLWGVLMAGDSSDYKGFVGRFRMRRAAECCRGIEEDVAAGRIPTDSLEARMLLAA
                     TRHLADRLGVSP"
     gene            3673..>4685
                     /locus_tag="AC16JC_17845"
     CDS             3673..>4685
                     /locus_tag="AC16JC_17845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018547503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA-like domain-containing protein"
                     /protein_id="HIR5166621.1"
                     /translation="MNVVDAAKAAFAAQGPAVTLGAVVHEGKCYPGPVVTLPLATLNR
                     HGLIAGATGTGKTKTLQLISEQLSAAGVPVCVADIKGDVSGIAAPGEPNDKIAQRAKD
                     TGYPWKPNGYPVEFLSLTGQRGAQLRATVSSFGPLLLAKVLGLNETQTSVLSLVFKYC
                     DDKGLLLLDFPDLRAVLQHLTGDGAGELKVYGGMAQTTVGVLLRDMIQLEQQGAEQFF
                     GEPEFELVDLMKTGPDGRGLVSVLELTDVQDRPAMFSTFMMWMLARLYHDLPEIGDPD
                     KPKLVFFFDEAHLLFDNASKQFLDQVQQVVRLIRSKGVGIFFVTQSPKDVPDDILGQL
                     GHKV"
BASE COUNT          747 a         1565 c         1646 g          727 t
ORIGIN      
        1 aacaggaccg cgggcaggat cccgtgctcg ccggcgcgcc ggcgcaaggg ctcggcctcc
       61 tctcccgtgc cgaccatgac gaacatgacg tcgtgccgtt gccggtggat gaggcgcgcg
      121 acatccagga acaggtccag atccttttcc ttcgcgaatc gacacgccgt accgacggtc
      181 accgtcgttt ccggaatgcc gagggcgtgc tttgcctcct gccggggctg ccgtggggag
      241 aaccggtcgg gatcgacggg gttggtgatc gtctcgatgc ggccacgctc caccccggcg
      301 gcaaccatgt cctccgcgat cttgccggac accgcgatga accggtcgaa cacgcggccg
      361 aacaccctgt agaacattcg cttccgggcg cgcagccggg ggatgttgat ccccccgtgc
      421 tcctggtgga tcgtgcgtgc cttggccgcc ccgaggcgga tcgccatggc ggccaggaag
      481 gtgcccgtgt ggtcgtgcac gacgtcgaat gcctcgcggc gcaggaaccg ggtgaaccgc
      541 agccaggcca ggaggtcacg accggagcgc gcccccatgg cggcgacccg gacgttctgg
      601 tccgagaggg cgtcgatgag ggggctcgat cgcgtcagga cgcacaccgt cagcgcatgg
      661 ttggccgcgg cggcgtgacg cgccagatcc accagattca gggcgatccc tcctacttcg
      721 ccccaccaga tgacgtgcag caccctcagc cggcgtgccg ccatggagcc tgggggaggt
      781 ccgtccggcg gaaccgtcac cgtctctcga tccggcgggc cgggcggtcg tccggatgaa
      841 gaccatcgcg atcgtcgcgg gcgctcggcc caatttcatg aagatcgcgc cgctcgtgcg
      901 cgcgctgcgc caggacggcg ggcagctcgg gttccagatc gtccacaccg ggcagcacta
      961 cgatccggcg atgagcgagg tcttcttcga ggagctcgcg attccccggc cggacgttgc
     1021 gctcggcgtc gggagcggct cgctcgccca gcagaccgcg aagatcatgg tggggttcga
     1081 gcggtactgc cgggcgcagg ccccgcacct ggtgctggtc gtgggggacg tcaactccac
     1141 gctggcgtgt tccgtcgtcg cgaagcagca gcacattccg gtggcgcacg tcgaggccgg
     1201 cctgcgctcg ggtgacatga ccatgccgga ggagatcaat cgcatcgtca ccgacgcgat
     1261 cgccgacctg ctgttcgtca ccgagccgag cggcgtcgcc aatctgctgc gcgagggcaa
     1321 gccgcgggag gtggtgcatc acgtcgggca cgtcatggtg gacaatctgc tctatcagct
     1381 cgaccggctc gcctgcgtcg atacctccgg gtacgtctcg tcggggatcc gcgcgcgata
     1441 cccccgctac ggcgccgtga cgctgcaccg tccgtccaat gtggactcgc cggaggtgtt
     1501 cacacggata tcctccgcgc tgcgcgagat ctcgcaccac ctgccgctgg tcttccccgt
     1561 gcacccccgg acgcgcgtga atctggagaa gtacggtctc gacctcggca gccgcgtgat
     1621 gctgacgccg cccctctcgt tcatggactt cctcaatctc tggacggagg ccacggtcgt
     1681 gctgaccgac agcggtggcc tgcaggagga gaccaccgcg ctcgggatcc cgtgtctcac
     1741 gctgcgcgac aataccgaac ggccgatcac cgtggacgaa gggacgaacg tggtggtggg
     1801 caccgatccg gcgcgcatca cggacgcggt ggtcacggtg ctgcgcgggg aaggaaagcg
     1861 ggggcgtcgt cccgagctgt gggatgggca cgcggcgcaa cggatcgccg tcgtgttacg
     1921 acgcgccctg gccgctcggt gagcgcctca tggctgctgc agccggtcgg ccagggccgt
     1981 gtacgccggc ttggggcgat agtcggcgtc cagcggcagg gcgtgcccgt atcccgggta
     2041 gaacttcggg atccagctgt agcggtccgt gaatccccag ctcgtgaacc cggcgcaccg
     2101 cgggcccacg cgcaggcagg cgtccaacat gtcgcggtag gccgtcgctt cctgggtcaa
     2161 cgccgtgccg gcagcactgt cggcaatgcg cacatccatc tcgctcacgc gcacgtcgaa
     2221 tcccgcctgc gcaaaccgcc cgagattggc ctggaggtcc gcggcggcgg gcgacggcag
     2281 gacgaaatgc gcctggaacc cgacgccgtc gacggggatc ccccgggccc tcagcccctg
     2341 cacgagcgcg aggagcgcgt tcgaccgggg gttgaccacc tccgcatgcg tctcgttcac
     2401 gtacaaggcc gcggccggat cggcgcgacg ggcccacaca aacgcggaat cgatgtactc
     2461 cgggccgatc acctgcagcc agagcgtgct gcgcaacggg gccgcggagc cgtcgacgac
     2521 ctcgttgacg acgtcccacg tcgcgatccg gccccggtag tggcccacga ccgtgctgat
     2581 gtggtcccgg agcacctgga gcagctgggt cttggtgaac gatcccgccg tgatccaggc
     2641 gggcagcgct tcgccccaca ccagcgtgtg gccatggatc gccatgcggt tggcctgggc
     2701 gaatgcgacg agccggtccg cgtcggcgaa gtcgtattgc tgctgaccgg ggtggaggga
     2761 tccgaacttc atcgcccact ccgaggtcac gccgttgtac tcggtcgcga gcttcacgcg
     2821 gtacagcgag tcgctccgca gcgcgttggg gtccaccgcc gtccccacca cgaagtgtcg
     2881 cgcggccgcg agctggcgca gcgggggcga ctgctccacg atcgtcgcgt cggtcgcggt
     2941 gggggcgggg gtgcagccgg ggccgagcca gaccaggatg gaggtgaggg ccaggaccga
     3001 atgacgagcg cacgtgccgg cagcacatgc gggatgagcg aggactggcc ccatggtgac
     3061 tccgttgttc gacggcccgg caggcgtcgt gaggtcccgc caacgggatt ctcgacgcgg
     3121 cggctggaaa ctagtgcttc gggatgcaaa agaaagcagc gcggagtttc aaggagaaac
     3181 cccgaggcgg tcggcgaggt ggcgggtcgc agccagcagc atccgcgctt ccagcgaatc
     3241 ggtgggaatc cggccggcgg cgacgtcctc ctcgatgccg cggcagcact ccgccgcccg
     3301 gcgcatgcgg aaccgtccca cgaatccctt gtagtcgctc gaatcccccg ccatgaggac
     3361 gccccacaac cggatgcgca cgtcgtcgac ggcgttcttg agctccgcga tgccttcctg
     3421 cggaaggtcg gcggtctgtt cgatgcggcg cgccatcgtg agaatggcgc tgcgcacctc
     3481 gcccaacgcc gggttcgcag cgggggtggt catcgagtcc acgaggttcg agtcgcccca
     3541 atgataggga tcgggccgcg gccgggcaac ggcgccttca ttgcgccttc gacgaacgca
     3601 tgcgaacgtt tggcacgaca tccttgcggg agactggatc atgcccggtc ggatcaacaa
     3661 agtggcggcg cagtgaacgt cgtcgatgcc gcgaaggcgg cgttcgccgc acagggaccc
     3721 gccgtgacgc tcggcgcggt ggtgcacgag gggaagtgtt atcccggccc cgtggtgacg
     3781 ctgccgctcg cgaccctgaa ccggcacggc ctcatcgccg gggccaccgg caccggcaag
     3841 accaagacgc tgcagctcat ctccgagcag ctgtccgcgg ccggagtgcc cgtgtgcgtc
     3901 gccgacatca agggtgacgt ctcggggatc gcggcgcccg gcgagcccaa cgacaagatc
     3961 gcccagcgcg ccaaggacac cggctacccg tggaagccga acgggtaccc ggtcgagttc
     4021 ctgagcctca ccgggcagcg cggggcgcag ctgcgcgcga cggtgtcgtc gttcgggccg
     4081 ctgctcctgg ccaaggtgct ggggctgaac gagacgcaga cgagcgtgct cagcctggtc
     4141 ttcaagtact gcgacgacaa gggtctgctg ttgctcgact tcccggatct gcgcgcggtg
     4201 ctgcagcacc tcacgggcga cggggcgggg gagctgaaag tctacggcgg gatggcgcag
     4261 accacggtcg gcgtgctgct gcgcgacatg atccagctgg aacaacaggg cgccgagcag
     4321 ttcttcgggg agccggagtt cgagctggtc gatctgatga agacgggtcc cgacggccgg
     4381 ggattggtga gcgtgctgga gctgaccgac gtgcaggacc gcccggcgat gttctccacc
     4441 ttcatgatgt ggatgctcgc ccggctgtac cacgacctgc cggagatcgg tgatcccgac
     4501 aagccgaagc tcgtcttctt cttcgacgaa gcgcacctgc tgttcgacaa cgccagcaag
     4561 cagtttctcg accaggtgca gcaggtggtg cgcctgatcc gctcgaaagg cgtgggcatc
     4621 ttcttcgtga cccagagtcc caaggacgtg ccggacgaca tcctcggcca gctggggcac
     4681 aaggt
//
LOCUS       DBNGZW010000764         3076 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1082938, whole genome shotgun sequence.
ACCESSION   DBNGZW010000764 DBNGZW010000000
VERSION     DBNGZW010000764.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1082938"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(186..2849)
                     /locus_tag="AC16JC_17850"
     CDS             complement(186..2849)
                     /locus_tag="AC16JC_17850"
                     /inference="COORDINATES: protein motif:HMM:NF039587.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HIR5166622.1"
                     /translation="MLILIVSAFALQGALPDSTALRHTGGVAPQVVAAARIDRAPILD
                     GRLDDPIWGTAAAVAELRQRDPDEGRAVSESTEVRVVYDGAALYVGARLFDRDPDAIA
                     RRLARRDVETPSDEFQVMLDSYHDHRTTFLFAVSPAGVQRDAVAGDDGDDFDSSWDPV
                     WQAVTSVDSLGWTVEIRIPFSQLRFSPAQAQLWGVRFVRWIQRKQELALYPFVPKTES
                     GVASRFAHLVGLRDITVPRPPEVVPYVVARGRYHRPAKLHNPFDDGSTTVGGAGMDLK
                     AGVSSNLTLDLTVNPDFGQVELDPSFVNLTEFEQFLPEQRPFFVEGADIFKFGGNGGG
                     LTRFSGAPLYYYSRRVGRPPQGEPTSHGQFKDEPVNTTILGAAKLSGKTSNGWSIGAV
                     EALTAREYATVIDTMTGGRFRNEVEPLTSYMTTRLKRNLHGGASSIGFLATAVNRDLY
                     DPALKFLRSAAYGGGMDFQHRWHDNTYGLAGSLGGSYIRGEPEAIQIAQRSSNRYFQR
                     PDSRHLRYDSTRTSLAGVSAEAYLSRLAGNWIWSAGGSTTSPGFEVNDLGFQQRVDRS
                     SAAAAGGRRWTRPGSTFRNAKATLTGTQSWNYDGNLIQRNVSAYAWGQFRNFWQADVN
                     LVANGSVIDDRLTRGGPLARKPPGWTATAEGYTDDRRRFTGYAYVSYTRNTAGGWYLS
                     TLPSITYRPSKASSVSLSIGYESGWSPAQFVKRVADTTATATLGTRYVFAAIRQNSLY
                     ATLRTNLTFSRALSLQFYAQPFTFLGDYAEFKELTARKTFTFNQYGRDNGSTISRSGD
                     TYTVDPDGPGPARAFTIGSPDFRERSLRVNTVLRWEYRPGSTIFLVWSQTRSNEFTDV
                     RDDVSHDFSQAVFHDRPTNVLLVKVSAWLRP"
     gene            complement(2843..>3076)
                     /locus_tag="AC16JC_17855"
     CDS             complement(2843..>3076)
                     /locus_tag="AC16JC_17855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166623.1"
                     /translation="ASGKPAVVQHTGPSSYLPDGEGLFRFATVEEAAAALEAVNADYE
                     RHCRAAREIAVTWFDAKQVLSHILNAALQEAAC"
BASE COUNT          461 a         1018 c         1095 g          502 t
ORIGIN      
        1 gggagacggg gcgctgcgca aacggatggg cgccgccggg cggcgggcgg tggagaccta
       61 ctccaactgg gaccgggtgt gccgggacat gatgcgcatc gacagggaat ttcgtcgagc
      121 cgtctcgacg gagcacagag cacggagcag tctgctccgt gccgtctgct ccctgctcct
      181 gccgttcacg gccgcaacca ggcactcact ttcacgagca acacgttggt cggccggtcg
      241 tggaacacgg cttggctgaa gtcgtgcgaa acgtcgtcac gtacgtccgt gaactcgttc
      301 gagcgcgtct ggctccacac caggaagatc gtcgagccgg gacggtactc ccaccgcagc
      361 accgtgttga cgcgcagcga gcgctcccgg aaatcggggc tcccgatcgt gaaggcccgc
      421 gccggtccgg ggccgtcggg gtcgaccgtg tacgtgtcgc cgctccggga gatggtcgag
      481 ccgttgtccc ggccgtactg gttgaaggtg aacgtcttgc gtgccgtgag ttccttgaac
      541 tccgcgtaat cgccgaggaa cgtgaacggc tgcgcgtaaa actgcaggct gagcgcccgc
      601 gagaaggtga gattggtgcg cagcgtggcg tagaggctgt tctgacggat cgccgcgaag
      661 acataccgtg tgcccagggt cgcggtcgcc gtagtgtcgg cgacgcgctt gacgaactgt
      721 gccgggctcc agccgctctc gtagccgatg ctgagactga ccgatgaggc cttgctggga
      781 cggtaggtga tcgagggcaa cgtgctcagg taccacccgc cggccgtgtt ccgggtgtag
      841 gacacgtacg cgtatccggt gaagcgccgg cggtcgtcgg tgtagccctc ggcggtggcc
      901 gtccaacccg gcggcttgcg tgccagcgga ccgccgcgcg tcagccggtc gtcgatcacc
      961 gagccgttgg cgacgaggtt cacgtcggcc tgccagaagt tccggaactg gccccacgcg
     1021 tacgcactca cattgcgctg gatcaggttc ccgtcgtaat tccagctctg cgtgccggtg
     1081 agggtcgcct tcgcgttgcg gaacgtggac cccggcctcg tccagcggcg tccgccggcg
     1141 gcggcggcgg aggagcggtc gacccgttgc tggaagccga gatcgttcac ttcgaagccc
     1201 ggggaggtcg tgctcccccc cgcgctccag atccagttgc ccgcgagcct gctgaggtag
     1261 gcctccgccg agacgccggc gagcgaggtc cgggtggaat cgtagcgcag gtggcggctg
     1321 tcggggcgct ggaagtagcg gttggaggag cgctgggcga tctggatcgc ttcgggctcg
     1381 ccccggatgt aggagccgcc caggctgccg gcgagcccgt aggtgttgtc gtgccagcgg
     1441 tgctggaagt ccatgccccc gccgtaggcg gccgatcgca ggaatttgag cgcgggatcg
     1501 tagaggtccc ggttgaccgc cgtggcgaga aacccgatgc tcgaggcccc gccgtggaga
     1561 ttgcgcttca accgggtcgt catgtagctc gtgagcggct cgacctcgtt gcggaagcgc
     1621 ccgccggtca tcgtgtcgat cacggtggcg tattcccgcg cggtcagcgc ctccacggcc
     1681 ccgatcgacc agccgttcga cgtcttgccg ctcagcttgg cggcgcccag gatcgtggtg
     1741 ttgaccggct cgtccttgaa ctggccgtgc gaggtcggct ctccctgggg cggccggccg
     1801 acgcgccgcg agtagtagta gagcggcgct ccgctgaaac gggtgagtcc gccgccgttg
     1861 ccgccgaact tgaagatgtc tgcgccctcc acgaagaacg ggcgttgctc cggcaggaac
     1921 tgctcgaact cggtcaggtt cacgaacgac gggtcgagct ccacctgccc gaagtccggg
     1981 ttgaccgtga ggtcgagggt gaggttcgag ctcacgccgg ccttcaggtc catccccgct
     2041 cccccgacgg tggtgctgcc gtcgtcgaac gggttgtgca gcttcgccgg tcgatggtag
     2101 cgtccccgcg cgaccacgta cgggaccacc tcgggggggc gcggcacggt gatgtcccgg
     2161 aggcccacga ggtgggcgaa gcgggacgcg acgccgctct ccgtcttcgg gacgaacgga
     2221 tagagcgcga gctcctgctt gcgctggatc cagcgtacga accggactcc ccagagctgc
     2281 gcctgcgccg gagagaagcg cagctgcgag aacggaatcc ggatttccac ggtccagccg
     2341 agcgaatcca cgctcgtcac cgcctgccac accggatccc aggacgaatc gaaatcgtcg
     2401 ccgtcgtctc ccgccacggc atcccgctgg acgcccgccg gactgaccgc gaacaggaag
     2461 gtagtgcggt gatcgtgata gctgtccagc atgacctgga actcgtcgct cggcgtttcg
     2521 acgtcgcgac gggcgagtcg ccgcgcgatg gcgtccggat cgcgatcgaa cagccgcgcg
     2581 cccacgtaga gcgccgcgcc gtcgtagacc acgcgcacct cggtggactc gctgaccgcg
     2641 cggccttcgt cgggatcccg ctgccgcaat tccgcgacgg ccgcggcggt gccccagatc
     2701 ggatcgtcga gccggccgtc caggatcggg gctctgtcga tccgcgccgc cgctaccacc
     2761 tggggcgcga cgccgccggt atgacgcaac gccgtcgagt cgggcaacgc gccctgcagg
     2821 gcgaaggccg agacgatgag gatcagcacg cggcctcctg cagcgccgcg ttgaggatgt
     2881 gcgacagcac ctgttttgcg tcgaaccagg tgacggcgat ctcgcgggcc gcccggcaat
     2941 gccgctcgta gtcggcgttc acggcttcca gcgcggccgc ggcctcctcc accgtggcga
     3001 accggaacag gccctcgccg tcggggaggt agctgctcgg gccggtgtgc tgcaccacgg
     3061 ccggtttgcc gctcgc
//
LOCUS       DBNGZW010000765         2010 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1022213, whole genome shotgun sequence.
ACCESSION   DBNGZW010000765 DBNGZW010000000
VERSION     DBNGZW010000765.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1022213"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="AC16JC_17860"
     CDS             complement(<1..287)
                     /locus_tag="AC16JC_17860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5166624.1"
                     /translation="MKLLVIEDDRTVGQYVKRGLTESGSQVDLVADGAEGLRLASDGH
                     YDVVVLDLRLPSMDGRDVLRTLRDRGNAVPVLVLTAQDAVDFKVQALRMGA"
     gene            complement(284..1840)
                     /locus_tag="AC16JC_17865"
     CDS             complement(284..1840)
                     /locus_tag="AC16JC_17865"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009399 - nitrogen fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     NtrB"
                     /protein_id="HIR5166625.1"
                     /translation="MAGSTLESPPAGLRVLADVVDILAAGFVTEDALVRVTAVLRRGL
                     DVADARLWLRSPTGGGFRLLAGSPPAGEGFAPAGAWFRQDLERERIAGGGVRLRFPLV
                     FEDQGVGALELRIAGGRSDEFPIDVGRVVARLLGAAIAATELSQDLASEVALAAREAD
                     AQRGFVALIIDSLPTGLYVIDRDYIIRAWNRQRESGTQGVARDDAVGREIFGVLDRQP
                     RELLRREFDRVFETGEIQQMEMESTASGESRYYRVSKIPMRLDNVHITHVITIAEDVT
                     EWRRAQQRLAQSEKLAAVGQLAAGVMHEINNPLATILACAEALSLRAEELPGTARAGF
                     EEYLKIIDTEVQRCRRIVDGLLDLSRPKTGEKRRVDVNHLVEQTLFLLKHHDRFKRIN
                     VRRQLAQGLPPVLVDPERLIQGFMSLMLNAMDAMNSRGVLTVRSSRTAGRGGGEVLVE
                     FIDTGTGIRQEELSKIFEPFYTTKPQGRGTGLGLSICYGIVADHNGRIEVESQVGIGS
                     NFKVYLPAAQ"
BASE COUNT          329 a          709 c          669 g          303 t
ORIGIN      
        1 tcggccccca tccgcagggc ctggaccttg aagtcgaccg cgtcctgagc cgtgagcacg
       61 agcaccggga cggcgttgcc gcggtcgcgc agggtgcgca gcacgtcccg tccatccatg
      121 gacggcaggc gcaagtcgag gactacgacg tcgtaatggc cgtcggaggc gaggcggagt
      181 ccttccgcgc cgtcagccac gagatccacc tggctaccgg actcggtgag cccgcgtttg
      241 acgtactggc ccaccgtccg gtcgtcctcg atgacgagca gtttcactgg gccgccggca
      301 gatagacttt gaagttggag ccgatcccca cctgggattc gacctcgata cgcccgttat
      361 ggtcggcgac gatgccgtag cagatggaca ggccgagtcc cgtcccgcgc ccctgcggct
      421 tcgtggtgta aaacggctcg aagatcttgg acagctcctc ctgccggatg ccggtgccgg
      481 tgtcgatgaa ctcgaccagg acttcgccgc cgccgcgacc cgcggtgcgg ctggagcgca
      541 ccgtgagcac gccgcgcgag ttcatcgcgt ccatcgcgtt cagcatcagc gacatgaagc
      601 cctggatgag ccgctccgga tccaccagta ccggcgggag accctgcgcg agctgacgcc
      661 ggacgttgat gcgcttgaac cggtcgtggt gcttcagcag gaacaacgtc tgctcgacca
      721 ggtgattcac gtccacccgc cgcttctcgc ccgtcttggg acgcgacagg tccagcaggc
      781 cgtccacgat gcggcggcag cgctgcactt cggtgtcgat gatcttcagg tactcttcga
      841 atcccgcgcg cgccgtgccg ggcagctcct cggcccggag actcagcgcc tcggcgcagg
      901 cgaggatcgt ggccagcgga ttgttgatct cgtgcatcac gccggccgcg agctgaccca
      961 cggcggcgag tttctcgctc tgcgccagcc gctgctgcgc gcgccgccac tcggtcacat
     1021 cctcggcgat ggtgatgacg tgcgtgatgt gcacgttgtc gaggcgcatc ggaatcttgg
     1081 acacgcggta gtagcgcgac tcaccgctcg cggtggattc catttccatc tgctggatct
     1141 caccggtctc gaacacgcga tcgaattcgc ggcgcagcag ctcgcgcggc tgccggtcca
     1201 gcacgccgaa gatctcccgc cccaccgcgt cgtcgcgcgc caccccctgg gtgccgctct
     1261 cacgctgccg gttccaggcc cggatgatgt agtcccggtc gatgacgtac agtccggtgg
     1321 gcagcgaatc gatgatcagg gcgacgaagc cgcgctgggc atcggcctcc cgtgccgcca
     1381 gcgccacctc ggaggcgagg tcctgggaca gttccgtcgc cgcgatggcc gcaccgagca
     1441 gccgggcgac gacgcggccg acgtcgatcg gaaactcgtc gctccgcccg cccgcgatcc
     1501 ggagctcgag cgcgcccacc ccctgatcct cgaacacgag cggaaatcgc agacgcacgc
     1561 ctccccccgc gatgcgctcg cgctccagat cctggcggaa ccacgcaccg gccggggcga
     1621 acccctcgcc ggcgggcggc gacccggcga ggagacggaa acctccaccc gttggagaac
     1681 gcagccagag ccgcgcatcg gccacgtcga gaccgcggcg cagcacggcc gtgacgcgca
     1741 cgagcgcgtc ttcggtcacg aaaccggcgg cgaggatgtc gacgacgtcc gcgagaacgc
     1801 ggagcccggc gggaggtgac tcgagggtcg agcctgccat tacctcgcta acctagggac
     1861 ccccccgggt atgcgccagc gggagctcga ttcgcagccc gagaaacgga tgcgcttcgg
     1921 tgggatggcc caggaaccag acgggcccga ggagaatggc gaaatgcatg gcgtcgtcct
     1981 gcccgatgcg gacggcgggc aacacgccca
//
LOCUS       DBNGZW010000766         3801 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1765792, whole genome shotgun sequence.
ACCESSION   DBNGZW010000766 DBNGZW010000000
VERSION     DBNGZW010000766.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 3801)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3801)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3801
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1765792"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(111..2537)
                     /gene="galB"
                     /locus_tag="AC16JC_17870"
     CDS             complement(111..2537)
                     /gene="galB"
                     /locus_tag="AC16JC_17870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015808569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004565 - beta-galactosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase GalB"
                     /protein_id="HIR5166626.1"
                     /translation="MTRRLVALLALAVAGLTLPLDGQRAMLDFDRDWRFHLGDVAGAE
                     AMGFPDTAWRPLDVPHDWSIEGEFSDTNPAGVAGGALPGGIGWYRKTFSVPVTDSGRL
                     VFVDFDGVYRNSQVWINGHDLGTRPYGYSSFQYELTPYIRYGPATNVIAVRVDNSRQP
                     NSRWYSGSGIYRHVRLVTTNPVHVDHWGTYVTTPQVSARSSRVHIRTTVRNESRDERR
                     LTVRTTIYDASGTVVAETSSAGRVPADSTLELAQELVVRQPQRWSIERPYLYRAVTRL
                     ECSGTVCDEYTTPFGIRSFVFRADSGFFLDGTHVKIRGVCLHHDLGALGAAVSDRALE
                     RQLEIMKAMGVNAIRTSHNPPAPELLDLADRMGFIVMDEAFDMWRKQKNDYDYHLDFA
                     TWHERDLSDMVRRDRNHASVFIWSIGNEVMEQWTNGDSTVGPLARELAGIVRHLDPTR
                     PITSANNNGSPQSEIFHAGALDLLGHNYHHEAWPIFPQQFPGQKFIITEAMSALNSRG
                     VYEQPSDSVAVYYTPYDTTKRPRPNPNGRISSYDNRRASWGSLHEESLRLFERYPFLS
                     GMFIWQGIDYLGEPTPYGWPARSSYFGIVDLAGFPKDPFYLYQSVWTTSPMLHLFPHW
                     NWAAGDTIDVWAYTNADEVELFLNGASQGVKKKEEPVSHLMWRVGYAPGTLRAVARKH
                     GQVTASAEVKTAGVPARIGLEPDRTSLQADGKDLSFVTVTVTDSAGVSVPTADHRIHF
                     RVDGDARIEGVDNGDQISHEPFKGDHVRLFNGKALVIIRAGRTPGTITLTATAEGLAP
                     ASVSIALRPR"
     gene            complement(2534..>3801)
                     /locus_tag="AC16JC_17875"
     CDS             complement(2534..>3801)
                     /locus_tag="AC16JC_17875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009096059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sodium:solute symporter family transporter"
                     /protein_id="HIR5166627.1"
                     /translation="VAVVVLTGLYTVAGGMRGVAYTEAAQTIILILGSVLITVFGLVR
                     LGGWHELRAALPSDLFNLWKPLVPAGMEGTWAPVKETGRIAWYFNGNYPWLGMLFCAP
                     IIGLWYWTTDQYIVQRALGAPNEQQARRGSIFAAYLKLLPVWIFIIPGMIALALARTG
                     RVPALTTMVDASGIAIPDAAQAAFPLMVKSILPAGVRGLVVAGLLAALMSSLAGAFNA
                     CSTLFTMDLYQKFRPGVSQHQLVWTGRVATAVMVLIGLLWIPVIHGGRGLYDYLQGVQ
                     GYLAPPIFTVFFFGVFMKRLNAQGALAALVTGFLLGAFRLIVDTPVSLGLAGLSHGYA
                     PGSLLWIVNNIYFQYYSLFIFVVSAVTMVVVSYLTPAPADQQLVGLTFATVTPEQRQQ
                     SRTSWSRSDVVASGVVLLLILAAYLYFRG"
BASE COUNT          659 a         1245 c         1287 g          610 t
ORIGIN      
        1 ccggaccaac ctttcggatc tcattgccgg ccatgggcaa cgttgtccgg gccgtgcggc
       61 ccccgcggcc tcgcgtcata gcccccactc ccggcgtcgc atcgactgtc ttaacggggc
      121 cgtagcgcga tgctcacaga cgcgggtgcg aggccttcgg cggtggccgt cagggtgatc
      181 gtcccaggcg tccggcccgc ccggatgatc accagcgcct tcccgttgaa cagtctgacg
      241 tggtcgccct tgaacggctc gtggctgatc tggtcgccgt tatccacacc ttcgatccgc
      301 gcgtcgccat cgacacggaa gtggatccgg tgatccgcag tcggcacgga gaccccggca
      361 ctatccgtca cggtgacggt gacgaatgaa agatccttgc catccgcctg aaggctcgtc
      421 cggtcgggct cgaggccgat ccgggccggc actccggcgg tcttcacctc cgcgctcgcc
      481 gtcacctgcc cgtgcttgcg cgccacggcc cgcagcgtgc ccggcgcgta gccgacccgc
      541 cacatgagat ggctcacggg ctcttccttc ttcttcacgc cctgcgaggc accgttcagg
      601 aacagctcga cttcgtccgc gttggtgtac gcccagacgt cgatcgtgtc accggctgcc
      661 cagttccagt gtgggaagag gtgcaacatc ggcgacgtcg tccacacgct ctggtacagg
      721 tagaaggggt ccttggggaa cccagccaga tcgacgatac cgaaatagga gctgcgcgcc
      781 ggccacccgt acggcgtggg ctcgcccagg tagtcgatcc cctgccagat gaacatcccc
      841 gagaggaacg ggtagcgctc gaacaggcgg agcgactcct cgtgcagcga gccccaggac
      901 gcgcggcggt tgtcgtacga cgagatccgg ccgttcgggt tcgggcgagg acgcttcgtc
      961 gtgtcgtacg gcgtgtagta aaccgcgacg ctgtcggagg gctgctcgta gacgccacgg
     1021 ctgttgaggg ccgacatcgc ttcggtaatg atgaacttct gtcccgggaa ctgctgcgga
     1081 aagatcggcc acgcctcgtg gtggtagttg tggcccaaga ggtcgagggc cccggcgtga
     1141 aagatctcgc tttgcgggct gccgttgttg ttcgctgacg tgatgggccg cgtggggtcg
     1201 aggtgtcgga cgatcccagc gagctcgcgc gccaggggac cgacggtgct gtccccgttg
     1261 gtccactgct ccatgacctc gttcccgatg ctccagatga agacgcttgc gtggttgcgg
     1321 tcgcggcgca ccatgtcgga caggtcgcgc tcgtgccagg tggcgaaatc gaggtggtag
     1381 tcgtagtcgt tcttctgctt ccgccacatg tcgaacgcct catccatcac gatgaagccc
     1441 atgcgatcgg cgaggtcgag cagctcgggc gccggcgggt tgtgcgacgt gcggatcgcg
     1501 ttgacgccca tcgccttcat gatctcgagc tgccgctcga gcgcgcggtc cgagaccgcc
     1561 gcccccagcg cccccaaatc gtggtgcaga cagacgccgc gaatcttaac gtgcgtgccg
     1621 tcgaggaaga acccgctgtc ggcgcggaac acgaagctgc ggatcccgaa cggcgtcgtg
     1681 tactcgtcgc acaccgtgcc gctgcattcg aggcgcgtga ccgcgcgata gagatacggc
     1741 cgctcgatcg accagcgctg cggctgccgc accacgagct cctgcgcgag ctccagggtc
     1801 gagtcggcgg ggacgcggcc tgccgaggag gtctcggcga cgaccgtacc agacgcatca
     1861 tagatcgtcg tgcgcacggt gaggcgccgt tcgtcccggg actcgttgcg caccgtggtg
     1921 cggatgtgga cccgtgacga tcgcgcactc acctggggag ttgtgacgta cgtaccccag
     1981 tggtccacgt gcactggatt ggtcgtgacc agtcgaacgt ggcggtagat gccggagccc
     2041 gagtaccacc gggagttggg ctggcgtgaa ttgtccaccc ggacggcaat gacgttcgtg
     2101 gccgggccat accggatgta gggcgtgagc tcgtactgga atgagctgta gccgtagggc
     2161 cgcgtgccca gatcatggcc gttgatccag acctggctgt tgcggtacac cccgtcgaaa
     2221 tcgacgaaca ccaaccgccc gctgtccgtc accggcacgg agaaggtctt gcggtaccat
     2281 ccgatgccgc ctggcagcgc accgccggcg acgccggccg ggttcgtgtc gctgaactca
     2341 ccctcgatgc tccagtcatg cggcacgtcg agcggtcgcc aggccgtatc ggggaaaccc
     2401 atcgcctcgg cgccggcaac gtcgccgaga tggaatcgcc agtcccgatc gaagtcgagc
     2461 atcgctcgct gcccatccag cgggagcgtg aggccggcga cagccagcgc gaggagggcg
     2521 acgaggcggc gggtcatccg cggaagtaca ggtaggccgc gagaatcagg agcagcacta
     2581 cgcccgaggc gacgacgtcg ctgcggctcc agctggtgcg cgactgctgg cgttgctccg
     2641 gcgtgaccgt cgcgaaggtg agcccgacga gctgctggtc agcgggcgcc ggcgtcaggt
     2701 aactcaccac caccatcgtc acggccgaga ccacgaagat gaagaggctg tagtactgga
     2761 agtagatgtt gttgacgatc cacaggagcg agcccggggc gtagccatgc gagaggccgg
     2821 cgagtccgag gctcaccggc gtatccacga tcaggcggaa cgcgcccagc agaaagcccg
     2881 tgacgagtgc cgccagcgcg ccctgcgcgt tcaggcgctt catgaagacg ccgaagaaga
     2941 acacggtgaa gattggcggc gcgagatagc cctgcacccc ctgcagatag tcgtacaggc
     3001 cgcggccgcc gtggatcacg ggaatccaga gcaatccgat gagcaccatg accgcagtgg
     3061 cgacccggcc ggtccagacg agctgatgct ggctcacgcc gggccggaac ttctggtaca
     3121 ggtccatcgt gaacagcgtc gagcaggcgt tgaaggcgcc cgcgagcgag ctcatcagcg
     3181 cggcgagcag ccccgcgacc accaggccgc gcacgccagc cgggaggatc gacttcacca
     3241 tcaacgggaa cgccgcctgg gcggcgtcgg gaatcgcgat ccccgaggcg tctaccatgg
     3301 tcgtgagcgc cggcacgcgt ccggtgcgtg cgagtgccag cgcgatcatg cctggaataa
     3361 tgaagatcca aacggggagc agcttcaggt acgcggcgaa gatggacccg cggcgggcct
     3421 gttgctcgtt cggcgcgccg agcgcgcgct gcacgatgta ctggtcggtg gtccagtacc
     3481 acagtccgat gatcggcgcg cagaagagca tcccgagcca cggatagttg ccgttgaagt
     3541 accacgcgat gcggccggtt tccttgaccg gcgcccacgt cccttccatc cccgccggca
     3601 cgagcggttt ccacaagttg aagagatccg acggcagcgc ggcgcgcagc tcgtgccacc
     3661 cgccgagccg caccaggccg aacacggtga tcagcaccga gccgagaatc aggatgatgg
     3721 tctgtgccgc ctcggtatag gcgacgccgc gcatcccgcc ggcgacggta tagagccccg
     3781 tcagcacgac gacggcgacc g
//
LOCUS       DBNGZW010000767         2294 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1834509, whole genome shotgun sequence.
ACCESSION   DBNGZW010000767 DBNGZW010000000
VERSION     DBNGZW010000767.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2294)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2294)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2294
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1834509"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="AC16JC_17880"
     CDS             <1..279
                     /locus_tag="AC16JC_17880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR5166628.1"
                     /translation="ACGGYGILLDIRDPANPRRLAAVADSNFAFWHSATFNNDGTQIL
                     FTDEWGGGLQPKCRATDDPKWGADALFTLKDSTLTFRSYYKLPAPQTS"
     gene            334..876
                     /locus_tag="AC16JC_17885"
     CDS             334..876
                     /locus_tag="AC16JC_17885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166629.1"
                     /translation="MVQGWYQGGVSVFDWTDAAHPREIAFFDRGPMDSTKLEGAGSWS
                     AYWDNGTVVSSEIGRGLDVLELLPNALLSKNEIDAAKLVRFDQVNVQDQQKLVWPASF
                     VVARAYLDQLERGNGLAAARVTATRSALARAERAAGQQRRAQLTQLAGQLDRDAAGAA
                     DQAKVRTLAAAVRNLAGARP"
     gene            902..1519
                     /locus_tag="AC16JC_17890"
     CDS             902..1519
                     /locus_tag="AC16JC_17890"
                     /inference="COORDINATES: protein motif:HMM:NF015658.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HIR5166630.1"
                     /translation="MNRRLSRALAAWLALAVSAGATRAAAQTVPDTGRFRYTAADVAF
                     MSGMLHPHAQAVLMAGWAASHGASAAVRTLCDRIVVAQRDEIALMQRWLRERNQPVPE
                     GDASHDMMPGMAHPLMPGMLTPEQLAQLDSARGPEFDRLFLTGMIRHHQGAITMVDQL
                     QSSQGAGEEETVFRFSSDVFADQTTEIGRMQRMLAALLFEGPSGQ"
     gene            1546..>2294
                     /locus_tag="AC16JC_17895"
     CDS             1546..>2294
                     /locus_tag="AC16JC_17895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LVIVD repeat-containing protein"
                     /protein_id="HIR5166631.1"
                     /translation="MVLTPPVRRLLRFVSLVGLGSLAACAASSSSTAGSQRVSTVAPA
                     PARDPRVGLRAGLMDAGEASWNLAVVSKNPQPEQFKGFTNSDLAFSGNYVIQGNYNGY
                     QVWDVTNPAAPVLKIGYVCPASQSDVSIYRNLLFVSGEGAGGRLDCGTQGVHDTVSAE
                     RLRGIRIFDVTDIGNPKYIANVQTCRGSHTHTVVEDPNDKDNVYIYVSGSAGVRSPNE
                     LAGCSKLAPDQDPNSALFRIEVIKVPLAHPE"
BASE COUNT          367 a          823 c          772 g          332 t
ORIGIN      
        1 gcgtgcgggg gctacggcat cctgctcgac atccgcgatc ccgcgaatcc ccggcgcctc
       61 gccgccgtcg ccgactcgaa cttcgcgttc tggcattcgg ccacgttcaa caacgacggg
      121 acccagatcc tcttcaccga tgaatggggc ggcgggctcc agcccaagtg ccgggcgacc
      181 gacgacccga agtggggggc ggacgcactc ttcaccctga aggacagcac cctcactttc
      241 cggagctact acaagctgcc ggcgccgcag acgtcgtagg agaactgcgt cgcgcacaac
      301 ggatcgctga tccccgttcc cggccgcgac atcatggtcc agggctggta ccagggcggc
      361 gtttcggtgt tcgactggac cgatgcggcg catccccggg agatcgcgtt cttcgaccgc
      421 gggccgatgg actccaccaa gctcgagggc gccggatcct ggtcggcgta ctgggacaac
      481 ggcaccgtcg tgagctcgga gatcggccgc gggctcgatg tcctcgagct gctgccgaac
      541 gccctgctct cgaagaacga gatcgacgcg gcgaagctgg tccgcttcga ccaggtgaac
      601 gtccaggacc agcagaagct cgtgtggccg gcgagcttcg tcgtggcgcg cgcctacctg
      661 gaccagctcg agcgcggcaa cggcctcgcg gcggcgcggg tgaccgcgac ccggagcgcc
      721 ctggcgcgcg ccgagcgagc cgccgggcag cagcgccggg cccagttgac gcagctggcc
      781 gggcagctgg accgcgatgc cgccggagct gccgatcagg cgaaggtgcg cacccttgcg
      841 gcagcggtgc gaaacctggc gggcgcgcgc ccgtaacacc cgcgcggaga ggagcgcata
      901 catgaataga cggctgtcac gcgcgctcgc agcgtggctg gcccttgcgg tctcggccgg
      961 cgcgacgcgg gctgcagcgc agaccgtgcc cgacaccggc cgcttccgct acaccgcggc
     1021 cgacgtggcc ttcatgtcgg ggatgctcca tccccacgcc caggcggtgc tcatggccgg
     1081 ctgggccgca tcgcacggcg cgagcgcggc cgtgcgcacc ttgtgcgacc gcatcgtcgt
     1141 cgcccagcgc gacgagatcg ccctgatgca gcggtggctg cgcgagcgca accagcccgt
     1201 ccccgagggg gacgcctccc acgacatgat gcccgggatg gcgcaccccc tgatgcccgg
     1261 gatgctgacg ccggagcagc tggcgcagct ggacagtgcc cggggaccgg agttcgaccg
     1321 gctgttcctg accggcatga tccgccacca ccagggcgcc atcacgatgg tcgaccagct
     1381 ccagagctca cagggggcgg gcgaggagga aacggtgttc cgcttctcct ccgacgtgtt
     1441 cgccgaccag accaccgaga tcggccgcat gcagcggatg ctggccgccc tgctgttcga
     1501 agggccgagc ggccaatgac tcccacccac cagaggaacg tgccaatggt cttgacccca
     1561 cccgtccgcc gtttgcttcg cttcgtctcg ctcgtcgggc tcggatcgct cgcggcgtgt
     1621 gctgcgtcgt cctcgtccac agcgggcagc cagcgcgtct cgaccgtggc acccgctccc
     1681 gcccgggatc cgcgcgtcgg tctgcgcgcc gggctcatgg acgccggcga agcgtcctgg
     1741 aacctcgcgg tggtctcgaa gaacccgcag ccggagcagt tcaaggggtt caccaactcc
     1801 gacctcgcct tcagcgggaa ctacgtcatt cagggcaact acaacggcta tcaggtgtgg
     1861 gacgtcacca acccggccgc gccggtgctg aagatcgggt atgtctgtcc cgcctcgcag
     1921 agcgacgtct cgatctacag gaacctgctg ttcgtctcgg gcgaaggcgc gggcgggcgg
     1981 ctcgactgcg gcacgcaggg cgtgcacgac acggtgagcg ccgagcgcct gcgcggcatc
     2041 cgcatcttcg acgtcaccga catcggcaat ccgaagtaca tcgcgaacgt gcagacgtgc
     2101 cggggctcgc acactcacac cgtggtcgag gaccccaacg acaaggacaa cgtctacatc
     2161 tacgtgtccg ggtcggccgg agtccgttcg cccaacgagc tggcgggctg ctcgaagctc
     2221 gcgcccgacc aggacccgaa ctcggcgctg ttccgcatcg aggtcatcaa ggtcccgctc
     2281 gcccaccccg agca
//
LOCUS       DBNGZW010000768         4622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1834544, whole genome shotgun sequence.
ACCESSION   DBNGZW010000768 DBNGZW010000000
VERSION     DBNGZW010000768.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4622
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1834544"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="AC16JC_17900"
     CDS             complement(<1..661)
                     /locus_tag="AC16JC_17900"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR5166632.1"
                     /translation="MLRIRAGMVFPVTAPPIEDGAVLIGADGRIAAVGPHARIPSPHN
                     AESLEFREAILTPGLVNVHTHLELTHLAGRNGEQDFVKWITRVREMKDATPPEEFQAA
                     AERGLRECWARGVTCVAETGSTGAAMQALVRFGGRGTVFQEVFGPAPAQVAASMAELE
                     QQVLRLRRLATSQRRVGVSPHAPYTVSEPLFRRVAHFARQEGLPIAVHVAESQEETEL
                     IR"
     gene            complement(666..2810)
                     /locus_tag="AC16JC_17905"
     CDS             complement(666..2810)
                     /locus_tag="AC16JC_17905"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HIR5166633.1"
                     /translation="MGRFAILKPQLSWETVLTRRGVAGGVALFVMLGLGFSWGAWSRV
                     CAAERCPTIARLMGSNSVPQQTSKVYAADGRLITELGLERRTVLPLREIPPWVREAFL
                     ATEDKRFYSHNGIDYWRIPGAFWANLSTLSYTQGFSTITMQLARNIFPEQISREKQLT
                     RKLKEARVAIEIEHNFPKDSILQMYLNQIDLGAGAHGVEAAAQVYFGKSARDLNVAEA
                     ATLAAIPKAPSFYNPRLHPDRAVRRRNVVLALMQSQGFLSADEAELWKAYPLVLTTSR
                     TNYGDVAPYFVEWLRTQYLEPRYGRDLYEKGLRIYTTLDLDMQEAAERALSAQLDAIE
                     AGVYTGGKWDRPTYRDYLESSKAGQGEDHGPATQYLQGALLAMDTRTGYIRAMIGGRD
                     FADSKFNRVTQAKRAPGSTFKPFVYSAAVRAGHPVTEILQDSPLDQPVLQPDSTYWNP
                     QDYDLTTKGPIPMRQSLFLSRNLSTIKLGMALGEQTVIGEARRYGITTPIPPVPAIHI
                     GAKEVYPIELVAAYSAFATLGTRAQPIGIIRVEDRNGNILWQPAVQREEVMDAAHEWL
                     MTDMLRDVVRRGTAASAVWGAGFQLPAAGKTGTTDDYTDAWYIGFTPELVAGIWVGYD
                     YLQRIMNDAGGGRIVAPAWTEFMRDVYDRRPAPADWARPDSLVVREVDWKTGYLATPF
                     CPAGDHHVDWFYPGTQPTQLCPVHGGIGPLTP"
     gene            2879..4138
                     /gene="tyrS"
                     /locus_tag="AC16JC_17910"
     CDS             2879..4138
                     /gene="tyrS"
                     /locus_tag="AC16JC_17910"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014099381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR5166634.1"
                     /translation="MSDLLAELRWRGLLHEATPGLDAHLVRGPLTAYCGFDPTARSLQ
                     LGNLVPLMLLQHLQRAGHRPIVLMGGGTGLIGDPSGKRTERPLLPAEELRANLEAQRA
                     QMAQFLDFSDDRALLIDNAEWLVPERLVDFLRNVGKHFTINIMLQKESVKARLESGLS
                     FTEFSYMLLQSYDYLQLYRRHQCTLQVGGSDQWGNITAGVDLVRRVEGAEVHGLVAPL
                     VTAPSGAKFGKSEAGAIYLDPALTSPFAFYQFWKNADDRDVGLYLRLFSLRPRTGPEG
                     IEALLDAHGRDPSRRDAQHKLAVELTERVHGKAATGAVVQAVSMLFGDWDPHRTDAAT
                     FRVLAREIPTATVPDGTALVDGIVASGLVKSKSEARRQIEQGGIYVNQQRAPDPNRAL
                     TTADWLAGGHVLLRKGKKDYALLQRAG"
     gene            complement(4146..>4622)
                     /locus_tag="AC16JC_17915"
     CDS             complement(4146..>4622)
                     /locus_tag="AC16JC_17915"
                     /EC_number="4.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR5166635.1"
                     /translation="YVRKELIPTIEPAMIGWMAFEGTDDFSRLTEYNPTFRADARRFE
                     MITLPYQDFYGFTQSVELLLEIGIPEIAEVTRSLHDPILTWADAHGMRVVSPRDERHR
                     SAILCIAPQEPVEAYHAIKRARIVCSLREGAIRLSPHCYNTVGEMEKVLDVLESLK"
BASE COUNT          691 a         1625 c         1585 g          721 t
ORIGIN      
        1 aacggatgag ctccgtctcc tcctgcgact ccgccacgtg aacggcgatg ggcaggcctt
       61 cctgccgcgc gaaatgcgcc acgcgccgaa acagcggctc gctcacggtg tacggcgcgt
      121 gcggcgacac gccgacacgc cgctgcgacg tggcgaggcg gcgcaggcgg agcacctgtt
      181 gctcgagctc cgccatgctc gccgccacct gggccggggc gggaccgaac acttcctgga
      241 acacggtgcc gcgtccgccg aagcggacca gcgcctgcat cgccgccccg gtgctgccgg
      301 tctccgcgac gcaggtgacc ccgcgggccc agcactcgcg caacccgcgc tccgcggccg
      361 cctggaactc ctccggtggg gtcgcgtcct tcatctcccg cacgcgcgtg atccatttca
      421 cgaagtcctg ctcgccgttg cggccggcga ggtgcgtcag ctcgagatgc gtgtggacgt
      481 tgacgagccc gggcgtgagg atcgcctcgc ggaactcgag cgactcggca ttgtgcggcg
      541 acgggatccg cgcgtgcggt cccacggcgg cgatccggcc gtcggcgccg atgagaacgg
      601 cgccgtcctc gatcggcggc gccgtgaccg gaaagaccat accggcgcgg atgcggagca
      661 tcgcgctagg gcgtgagcgg gccgatcccg ccgtgcaccg gacacagctg ggtcggttgg
      721 gtcccggggt agaaccagtc cacgtggtgg tcgcccgcgg gacagaaggg cgtggcgagg
      781 taccccgtct tccagtccac ctcccgtacc acgagcgagt cgggccgcgc ccagtccgcc
      841 ggcgccgggc ggcggtcgta gacgtcccgc atgaattcgg tccacgcggg cgcgacgatg
      901 cggccgccac ccgcgtcgtt catgatgcgc tgcaggtagt cgtagcccac ccagatcccg
      961 gcgacgagct ccggcgtgaa gccgatgtac caggcgtcgg tgtagtcgtc ggtcgtcccg
     1021 gtcttccccg ccgccggcag ctggaatccc gccccccaca cggcgctggc cgccgtgccg
     1081 cggcgcacca cgtcgcgcag catgtcggtc atgagccact cgtgggccgc gtccatgacc
     1141 tcttcccgct gcaccgcggg ctgccacaag atgttgccgt tgcggtcctc cacccggatg
     1201 atcccgatcg gctgggcacg cgtgccgagg gtggcgaagg cggaataggc ggccaccagc
     1261 tcgatcgggt acacctcctt cgccccgatg tggatcgccg gaaccggggg gatcggcgtg
     1321 gtgatgccgt agcgccgggc ttcgccgatg accgtctgct cgcccagggc cattcccagt
     1381 ttgatcgtcg acaggttgcg cgacagaaac agggactgcc gcatgggaat cggacccttg
     1441 gtcgtcagat cgtagtcctg gggattccag tacgtgctgt cgggctgcag caccggctga
     1501 tcgagcggcg agtcctggag gatctccgtc accgggtgcc ccgcgcgcac ggccgccgag
     1561 tagacgaagg gcttgaaggt cgagccgggc gcccgcttcg cctgggtgac ccggttgaac
     1621 ttggagtcgg cgaagtcgcg cccgccgatc atcgcccgga tgtacccggt gcgcgtgtcc
     1681 atggcgagga gcgcgccctg caggtactgc gtcgccggcc cgtggtcttc cccctgcccg
     1741 gccttggacg attcgaggta gtcgcggtag gtgggccggt cccacttccc gccggtgtac
     1801 acccccgcct cgatggcgtc gagttgcgcc gacagggccc gctcggccgc ctcctgcatg
     1861 tccagatcga gggtggtgta gatccgcagt cccttttcgt agaggtcgcg gccgtagcgc
     1921 ggctcgagat actgcgtccg cagccattcg acgaaatacg gcgccacgtc gccgtagttg
     1981 gtgcggctgg tggtgagcac cagcgggtag gccttccaca gctccgcttc gtcggccgac
     2041 aggaagccct gcgactgcat cagcgccagc acgacgttgc ggcgccgcac cgcgcggtcc
     2101 gggtgcagcc gcggattgta gaacgacggc gccttgggga tcgcggcgag cgtcgccgcc
     2161 tccgcgacgt tcaggtcgcg cgccgacttg ccgaagtaca cctgcgccgc ggcctccacc
     2221 ccgtgcgccc ccgcccccag gtcgatctgg ttcaggtaca tctggaggat cgagtccttg
     2281 gggaagttgt gctcgatctc gatcgcgacc cgcgcctcct tgagcttgcg ggtcagttgc
     2341 ttctcgcgac tgatctgctc ggggaagatg ttccgcgcga gctgcatcgt gatcgtggag
     2401 aacccctgcg tgtagctgag cgtgctgagg ttcgcccaga acgcccccgg gatgcgccag
     2461 tagtcgatgc cgttgtggct gtagaaccgc ttgtcctcgg tggcgaggaa cgcttcgcgc
     2521 acccagggcg ggatctcgcg cagcggcagg acggtgcgcc gctcgagacc gagctcggtg
     2581 atcaggcgcc cgtcggccgc gtagactttg gatgtctgct ggggaaccga gttggagccc
     2641 atcaaccgcg cgatggtggg gcagcgctcg gcggcgcaca cccgcgacca ggcgccccag
     2701 gagaagccga ggcccagcat gacgaagagc gccacgcctc cggccacacc ccgccgggtg
     2761 agcaccgtct cccacgacag ctgcggcttg agaatggcga agcgacccat cgccccacaa
     2821 cctagcgggg gcccccggcg cttgccagcg cccgcgcccg ctcttacgat tcgcgcccat
     2881 gtcggacctg ctcgcggaac tccggtggcg cggcctgttg cacgaggcga cgcccggcct
     2941 cgacgctcac ctggtgcgcg ggccgctcac cgcctactgc ggtttcgacc cgaccgcccg
     3001 cagcctgcag ctcggcaacc tggtcccgct gatgctgctg cagcacctcc agcgggccgg
     3061 ccaccggccc atcgtgctga tgggcggcgg caccgggctg atcggcgacc cctccggcaa
     3121 gcggacggag cgacccctgc tccctgccga ggagctgcgc gcgaacctcg aggcgcaacg
     3181 cgcccagatg gcgcagttcc tcgacttcag cgacgaccgc gccctcctga tcgacaacgc
     3241 ggaatggctc gtgccggagc ggttggtcga tttcctgcgt aacgtcggga agcacttcac
     3301 catcaacatc atgctccaga aggaatcggt gaaagccagg ctcgagagcg ggctgtcgtt
     3361 caccgaattc agctacatgc tgctccagtc ctacgactac ctgcagctgt accggcgtca
     3421 ccagtgcacc ctgcaggtgg gcggctcgga ccagtggggc aacatcaccg cgggcgtcga
     3481 cctggtgcgg cgggtggagg gcgcggaggt gcacggtctg gtggcgccgc tcgtcacggc
     3541 cccctccggc gcgaaattcg ggaaatcgga agccggcgcc atctacctcg atccggcact
     3601 cacgtcgccg ttcgccttct accagttctg gaagaacgcg gacgaccgtg acgtcggcct
     3661 gtacctgcgg ctcttctcgc tgcgcccgcg cacgggcccg gagggaatcg aagccctgct
     3721 ggacgcacac ggccgggatc catcgcggcg cgatgcgcag cataagctcg ccgtcgaatt
     3781 gaccgaacgg gtccacggga aggcggcgac gggcgccgtg gtccaggccg tctccatgct
     3841 gttcggcgac tgggacccgc accgcaccga cgccgcgacg ttccgggtgc tcgcccgcga
     3901 gatccccacg gccaccgtcc cggacggcac cgccctggtg gacggcatcg tcgcctccgg
     3961 gctcgtcaag tccaagagcg aggcacggcg tcagatcgag caggggggga tctacgtcaa
     4021 tcagcagcgc gccccggacc ccaaccgggc gctcaccacc gccgactggc tcgccggcgg
     4081 ccacgtgctg ctcagaaaag gaaagaagga ctacgcgctg ttgcagcgcg ccggttgacc
     4141 cgcggctact tcaggctctc caacacgtcc agcaccttct ccatctctcc caccgtgttg
     4201 tagcagtggg gggagagtcg gatcgcgccc tcgcgcagcg agcacacgat ccgggcgcgc
     4261 ttgatggcgt ggtaggcctc gaccggctcc tggggggcga tgcagagaat cgccgagcgg
     4321 tgccgctcgt cgcgcggcga aacgacccgc atcccgtgcg cgtctgccca cgtgaggatc
     4381 gggtcgtgca ggctgcgcgt gacctcggcg atttcgggga tgccgatctc cagcagcagc
     4441 tccaccgact gggtgaagcc gtagaagtcc tggtagggca gcgtgatcat ctcgaagcgg
     4501 cgggcgtcgg cgcggaacgt cgggttgtac tcggtgagcc gcgagaagtc gtcggtgccc
     4561 tcgaacgcca tccacccgat catcgccggc tcgatcgtcg ggatcagctc cttgcggacg
     4621 ta
//
LOCUS       DBNGZW010000769         2126 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1084512, whole genome shotgun sequence.
ACCESSION   DBNGZW010000769 DBNGZW010000000
VERSION     DBNGZW010000769.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2126)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2126)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2126
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1084512"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /gene="recO"
                     /locus_tag="AC16JC_17920"
     CDS             complement(<1..391)
                     /gene="recO"
                     /locus_tag="AC16JC_17920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HIR5166636.1"
                     /translation="MSLVTTAAVVLRTYRYSETSKIVRLATRDLGVQSAIAKGVSRPK
                     SRFGAGLELLSEGEAQLYHRDNRELQTLAAFDVERLRRELAGDLDRFAAAMVLAELMV
                     RMAPPAPLPALYDSFTGALDDLVVVPGD"
     gene            complement(412..>2126)
                     /locus_tag="AC16JC_17925"
     CDS             complement(412..>2126)
                     /locus_tag="AC16JC_17925"
                     /inference="COORDINATES: protein motif:HMM:NF012801.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HIR5166637.1"
                     /translation="RGIATVADALRLTPGAAVVASGSFGGQISVFVRGGESDYVKVLI
                     DGVPQNQPGGFYDFANLGTEDVERIEIVRGPVSVLYGSDAVAGVVQVFTRTGLGRPSG
                     RLAFGGGSYGTEWSTGEVSGATGPFAWRSTVSRRTTSGTFPVNNEDRRLLLGASVRFT
                     PDARTAAGLSFHYGDAVYHFPTDGAGQVSDSNQSTTDRGPSLAAEATHAFSERVSARA
                     SLDWHREDSRFDDAADSPGDTSAFCCFHSRDLVQRLLVGARADVRLPPGATVTVGVER
                     ELQRQTGTTLDTARHNTGLYAQVLAGLGTAVTLTTGARVDDNGQFGSHLTGRAGVAWQ
                     AARATRLRVSLGTGFKEPSFFENFATGFSRGNPALRPERSRSWEVGVERNLAGDRFVV
                     QATWFDQRFRDFIQYSPAPIGADSVNYRNVGEASARGLELSMHAAPGGGMTLDAAYTY
                     LRSRDAGTQQRLLRRPTHSGSVQVGHRFAGRGDLAVAARYTGDRADQDFSSFPAALVI
                     LPSHTVIDASAELRLTSRRGTLPEVRLTGRVENLFGARYEEVRNFPAPRRTLLVGALV
                     TFGR"
BASE COUNT          334 a          783 c          711 g          298 t
ORIGIN      
        1 tgtcccccgg aaccacgacc aggtcgtcga gcgcaccggt gaacgaatcg tacagcgcgg
       61 ggagcggcgc cgggggcgcc atgcgcacca tcagctcggc cagcaccatg gcggcggcga
      121 agcgatccag atccccggcc agctcgcgac gcagccgctc cacatcgaac gccgcgagcg
      181 tttgcagctc ccggttgtcg cgatggtaga gctgcgcctc accctcgctc agcagctcga
      241 gtcccgcccc gaaccggctc ttggggcgcg aaacaccctt ggcgatggcg ctctgtaccc
      301 cgaggtcccg cgtcgcaaga cgcacgatct tcgacgtctc actgtagcga taggtgcgca
      361 gcacgacagc cgcagtcgtg accagggaca tgggtggaag atagcgcccc gctacctccc
      421 gaaggtcacc agggccccca cgagcaaagt gcggcgcggt gccgggaaat tccgcacttc
      481 ctcgtaccgc gcgccgaaca agttctcgac ccgacccgtg agccgcacct ccggcaacgt
      541 gccacggcgt gaagtgagac ggagctcggc gctggcgtcg atcacggtgt gcgacggcag
      601 gatcaccaat gccgcgggaa acgacgagaa gtcctgatcc gcgcgatcgc ccgtgtagcg
      661 ggcggcgacg gcaaggtctc cgcgcccggc gaagcggtgg ccgacctgca cgctgccgct
      721 gtgcgtcggg cggcggagca gccgctgctg cgtccccgcg tcacgcgagc ggaggtaggt
      781 gtacgccgcg tccagcgtca tcccgccgcc gggtgcggcg tgcatcgaga gctcgaggcc
      841 gcgggcgctg gcctccccga cgttgcggta gttcaccgag tcggcaccga tcggcgcggg
      901 gctgtactgg atgaaatccc ggaaccgctg gtcgaaccag gttgcctgca cgacgaaccg
      961 gtcgcccgcc agattccgct ccacgccgac ctcccagctc cgcgaccgct ctgggcgcag
     1021 cgccgggttg ccgcgggaga atcccgtcgc gaagttctca aagaacgacg gctccttgaa
     1081 cccggtgcca agcgagacgc gcagccgggt cgcgcgcgcg gcctgccagg cgaccccggc
     1141 ccggccggtc aggtggctgc cgaattgccc gttgtcgtct actctggcgc cggtggtgag
     1201 cgtgaccgcc gtccccagcc cggcgaggac ctgtgcgtag agccccgtat tgtgccgtgc
     1261 cgtatcgagg gtggtcccgg tttgccgctg cagctcccgc tccacgccca cggtgacggt
     1321 cgcccccggc ggcaggcgga cgtcggcgcg ggccccgacc agcaggcgct gcaccagatc
     1381 ccggctgtgg aagcagcaga acgcgctcgt gtcaccggga ctgtcggccg catcgtcgaa
     1441 ccggctgtct tcccggtgcc agtcgaggct cgcccgggcg ctcacccgct ccgaaaacgc
     1501 gtgcgtcgcc tccgcggcca gcgacggccc ccggtcggtc gtggactggt tcgagtcgga
     1561 gacctgccct gcaccgtccg tcgggaaatg gtagacggcg tcgccatagt gaaacgagag
     1621 accggcggcg gtgcgggcgt ccggggtgaa acgcacgctg gcccccagca gcagcctccg
     1681 gtcctcattg ttgaccggaa acgtgccgga ggtcgtgcgg cgcgacaccg tgctccgcca
     1741 ggcgaacggc ccggtcgctc cgctcacctc tcccgtgctc cactcggtgc cgtagctgcc
     1801 tccaccgaac gccagtcggc cgctcggccg accaagcccg gtccgcgtga acacctgcac
     1861 cactccggcc accgcatcgg atccgtacag cacgctcacc ggaccgcgca cgatctcgat
     1921 gcgttccacg tcttcggtcc ccaagttggc aaagtcgtag aagccgccgg gctggttctg
     1981 cggcacacca tcgatcagca ctttcacgta atcgctctct cccccccgca cgaacacgga
     2041 gatctgcccg ccgaaggagc cggaggcgac caccgccgcg ccgggcgtga gacgcagcgc
     2101 gtcggccacc gtggcgatac cgcggg
//
LOCUS       DBNGZW010000770         5585 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_472677, whole genome shotgun sequence.
ACCESSION   DBNGZW010000770 DBNGZW010000000
VERSION     DBNGZW010000770.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_472677"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..380)
                     /locus_tag="AC16JC_17930"
     CDS             complement(<1..380)
                     /locus_tag="AC16JC_17930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166638.1"
                     /translation="MLSALERGPRARRVPRPARSLRQEYEEFLFQRIEEYKNALTRAE
                     LLEIGDEAVRELDVAAADQYLLTEVLLLEHVDRIIARRLRLPSFQRWSRTHRALRAAQ
                     REPTHWEIEATSPLVPWARRLEPG"
     gene            complement(374..1213)
                     /locus_tag="AC16JC_17935"
     CDS             complement(374..1213)
                     /locus_tag="AC16JC_17935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR5166639.1"
                     /translation="MVRRVLIGLFFANLAVVAAKVGVGVAARSLAVLGDALHSSVDAL
                     NNILALVVVRVAAQAPDEDHPYGHEKFETLGALAIVGFLSITCFELARDAVTRLLGPH
                     TVPRVSGAQLLILVGGLGINALVAWYEHRRGEELQSELLVSDAAHTRSDVFITAGVIV
                     GLLLSRRGWWWADPVLALLIAALIVRVAYRIIQRTVPILVDERALPRGEIQSVAEGVD
                     GVRGAYDIRSRGGGQARYAELTIAVDPATNVAAAHAIADEVEHRLKRDLRLGAVTVHV
                     EPC"
     gene            complement(1347..2525)
                     /locus_tag="AC16JC_17940"
     CDS             complement(1347..2525)
                     /locus_tag="AC16JC_17940"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HIR5166640.1"
                     /translation="MDSDKATATVTAKGAARWAAGHPWIYRSDVVAAPRGPAAPGIVR
                     VRDRRGAPLGQALYSPRSEIRLRLLSTGDDPIDTAWWRFRIAACAARREGIPATAYRV
                     VHAEGDGLPSLVVDRYGPFVVAQLLSAGLEAVRDDVLTGLRQALEPQGILLRHDAAVR
                     RHEGLPLEVEAAYGEVPEAVEVREGTVRYLAAPRHGQKTGAFLDQRDNRFLVGDHTRT
                     GGRALDVFTYHGSFALHLARHAVTVLAVDQSAEALARGAQNASLNSLGNISWREANAF
                     DQLRALERAGERFDTIVLDPPAFAKARASLPRALAGYKEINLRAMRLLAPGGVLFTCS
                     CSYHVHRADFLEMLGAAAADSGRRLELVRLTGAATDHPEVLNIPETGYLKGALLRALE
                     "
     gene            2524..3195
                     /gene="thiE"
                     /locus_tag="AC16JC_17945"
     CDS             2524..3195
                     /gene="thiE"
                     /locus_tag="AC16JC_17945"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HIR5166641.1"
                     /translation="MTTSLADRLRLMVITDRTLLRGRDPVAVCRAAVRGGATMIQVRD
                     KDAPPRDLLALVRSLVGGLPVPVLVNDRVDVALAAGAAGAHLGQDDVPIGAVRPHVPP
                     GFILGTSVGSAGEAGRARAEPADYWSIGPCFATASKSDAGPPLGPSGFAALARLAPPA
                     MALIGIGGIGPGNASLILRAGAAGVAVIAAVLGVEDPESAARALVSPAPAPSPGGSHP
                     GAPGS"
     gene            complement(3083..4132)
                     /gene="mltG"
                     /locus_tag="AC16JC_17950"
     CDS             complement(3083..4132)
                     /gene="mltG"
                     /locus_tag="AC16JC_17950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR5166642.1"
                     /translation="MRRRARGLLVAALLCSACRVQPSVPCDAAGPECVVLTIPRGATL
                     SAAVDSLTAHQIVAPRVLFKLYARLRGLGGSLKSGIYAFRRDTRWTDIVAALQLGRGI
                     LVRFTVREGLQVGEIADIAARQLQVPRDSFLAATRDAELLALLGGSAHAETAEGYLYP
                     TTYTVRPDLSGRELVRLMIEEFLRHWPPAWDARLDSMQMSLHDLVTLASIIESEVRYA
                     PDREYVSAVYHNRLRLGMLLQADPTVVYAMGRRPRRVYEKNLLIQSPYNTYLHPGLPP
                     GPISQPDSSSIAAALFPARVPFLYFVAQPDGKHIFSVSYQEHLDAIRQVKEQVQEIRE
                     RARRTRDPQPRAQRR"
     gene            complement(4129..4572)
                     /gene="ruvX"
                     /locus_tag="AC16JC_17955"
     CDS             complement(4129..4572)
                     /gene="ruvX"
                     /locus_tag="AC16JC_17955"
                     /inference="COORDINATES: protein motif:HMM:NF015607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR5166643.1"
                     /translation="MDGWTAGRLLGVDWGERRIGLALSDETQTLAQPLATLTRRTGRR
                     FPMKDLLTQLERHQVTGVVVGLPLEASGAEGEPARAARALAADIARRSGRPVDLVDER
                     FTTARVRRAVHEMGGRTEGRAGDVDALAATLLLQHYLDSRRGSSV"
     gene            complement(4577..>5585)
                     /gene="rho"
                     /locus_tag="AC16JC_17960"
     CDS             complement(4577..>5585)
                     /gene="rho"
                     /locus_tag="AC16JC_17960"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014811773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR5166644.1"
                     /translation="PSQIKRFDLRTGDTILGQVRPPKEGERYLALLKVETVNGEEPEK
                     AKHRVAFDNLRPRYPEERIRLERKDGDLSMRVMDILCPIGKGQRGLIVSPPKAGKTIL
                     MQKLANSIIENHPEIYLIVLLIDERPEEVTDMEENVKSAEVISSTFDEPADRHVQVAD
                     MVIEKAKRMVEHGKDVVILLDSITRLGRAHNVVVPHSGKILSGGVDANALQKPKRFFG
                     AARNIEGGGSLTILATALIDTGSRMDEVIFEEFKGTGNMEVILDRRIADRRVYPAIDV
                     QRTSTRKEELLLAKEELNKVYLLRNFLADMPPVEAIEFLLERMKRTKSNQEFFATMSQ
                     G"
BASE COUNT          790 a         1917 c         2031 g          847 t
ORIGIN      
        1 tcgcccggct cgaggcggcg cgcccagggg accagggggc tcgtggcttc gatctcccag
       61 tgggtcggct cgcgctgcgc ggcgcgcagg gcgcgatggg tgcgggacca gcgctggaaa
      121 ctcgggagcc gcagtcgccg ggcgatgatg cggtcgacgt gctcgagcag cagcacctcg
      181 gtgagcagat actgatcggc cgccgcgacg tcgagctccc gcaccgcctc gtcgccgatc
      241 tcgaggagct cggcgcgcgt cagggcgttc ttgtactcct cgatgcgctg gaacagaaac
      301 tcctcgtact cctggcggag cgagcgggcg ggacgcggga cccggcgggc gcgcggcccc
      361 cgctcgaggg cgctcagcat ggctcgacgt gcaccgtgac ggcgccgagc cgcaggtcgc
      421 gcttcaaccg gtgctcgact tcgtcggcga tcgcatgtgc ggcggcgacg ttggtcgccg
      481 ggtccaccgc gatggtcagc tccgcgtacc gcgcctgtcc gccgccgcgg gaacggatgt
      541 catacgcgcc acgcacgccg tccacgcctt ccgccacgga ttgaatctcc ccgcggggga
      601 gggcccgctc gtcgaccagg atcggcaccg tgcgctggat gatccggtac gccacgcgta
      661 cgatgagggc ggcgatcagc aacgcgagca ccgggtcggc ccaccaccag ccgcggcgcg
      721 acagcagcag ccccacgatc acgccggcgg tgatgaagac gtcgctgcgg gtgtgggccg
      781 cgtcggacac gagcagctcg ctctgcagct cctctccgcg gcgatgctcg taccacgcca
      841 ccagggcgtt gatgccgagg ccgccgacca ggatcagcag ctgggcgccg ctcacccgtg
      901 gcaccgtgtg gggccccagc aggcgcgtca ccgcgtcgcg cgccagctcg aagcaggtga
      961 tcgacagaaa gccgacgatc gccagcgcgc ccagcgtctc gaacttctcg tggccgtagg
     1021 gatggtcttc gtcgggggcc tgtgcggcca cgcgcaccac gaccagggcg aggatgttgt
     1081 tgagggcgtc gacggaggag tgcagggcat cgccgaggac ggcgagcgag cgggcggcca
     1141 cgccgacccc aaccttggcg gcgacgacgg ccaggttggc gaagaacagg ccgatgagca
     1201 cacgccggac caaggctgtc cggtcgtgcg cccccccctc acgccgggcg tgccggacgg
     1261 ggcggggccg cgggggccgg gcggctggca tggatgcaag cttgtaaggc gggcgcggag
     1321 agtcaacggg gagcgagcac gccttgtcat tcgagcgccc tcagcaaggc ccccttcagg
     1381 taccccgtct ccgggatgtt caggacctcc ggatggtccg ttgccgcgcc ggtgagtcgc
     1441 accagctcga ggcggcggcc gctgtccgct gcggcggccc ccagcatttc caggaaatcg
     1501 gcgcgatgga catggtagga acaggagcac gtgaaaagca cgcctccggg cgcgagcagg
     1561 cgcatggccc ggaggttgat ctctttgtag ccggcgaggg cgcggggcag ggacgcgcgg
     1621 gccttggcga acgcgggcgg atcgagcacg atggtgtcga acctttcgcc ggcgcgctcc
     1681 agcgcccgta actggtcgaa cgcgttggcc tcgcgccagg aaatgttgcc gaggctgttg
     1741 agcgacgcgt tctgtgcgcc gcgggccagg gcctcggcgc tctggtccac ggccaggacc
     1801 gtcacggcat gccgcgccag gtgcagggcg aaagatccat ggtaggtgaa cacatcgagc
     1861 gcccgccccc ccgttcgcgt gtggtccccc acgaggaagc gattgtcccg ctggtcgaga
     1921 aacgcaccgg tcttctgccc gtgccgcggg gcggcgaggt agcggacggt cccttcccgc
     1981 acttccaccg cctcgggcac ctcgccgtag gccgcctcga cctcgagcgg cagtccctcg
     2041 tggcgtcgca ccgccgcgtc gtggcgcaac aggatcccct gcggctcgag cgcctgccgc
     2101 agaccggtga gtacgtcgtc acggaccgcc tccagtccgg cggagagcag ctgcgccacg
     2161 acgaaggggc cgtagcgatc gaccaccagg gatggcagac cgtccccctc cgcgtgcacc
     2221 acccggtagg cggtggccgg aatcccctcg cgacgggcgg cgcacgccgc gatgcggaag
     2281 cgccaccagg cggtgtcgat cgggtcgtcg cccgtgctga gcagccgcaa gcggatttcg
     2341 ctgcgtgggg aatacagggc ctggccgagc ggtgcgcccc gccggtcgcg cacgcgaacg
     2401 atcccggggg cggccgggcc gcgcggcgcc gcgaccacgt cgctgcggta gatccaggga
     2461 tgaccggcgg cccagcgcgc ggcgcccttg gcggtcacgg tcgcggtagc tttgtccgaa
     2521 tccatgacca cctccctcgc cgaccggctc cgcctgatgg tgatcaccga ccggacgctg
     2581 ctccgggggc gcgatccggt cgccgtgtgc cgcgcggcgg tgcggggcgg tgccacgatg
     2641 atccaggtgc gggacaagga tgcgccgccg cgcgacctgc tcgccctcgt ccggtccctg
     2701 gtcggtgggc tgcctgttcc cgtgctggtc aacgaccgcg tggacgtcgc gctcgccgcc
     2761 ggcgcggccg gcgcgcacct cggacaggac gacgtgccga tcggtgcggt gcggccccat
     2821 gtgccgccgg gattcatcct cggcacttcc gtcggctccg cgggggaggc cggccgggcc
     2881 cgcgcggagc cggcggatta ctggagcatc ggcccctgct tcgcgaccgc cagcaagtcc
     2941 gatgcgggtc ctcccctcgg gccctcgggc ttcgccgcac tggcgcggct cgcgcctccc
     3001 gccatggcgc tgatcggtat cggtggcatc ggacccggca atgcgtcgct catcctgcgg
     3061 gcgggcgcgg ccggcgtcgc ggtcatcgcc gctgtgctcg gggttgagga tcccgagtcc
     3121 gccgcgcgcg ctctcgtatc tcctgcacct gctccttcac ctggcggatc gcatccaggt
     3181 gctcctggta gctgaccgag aagatgtgct tgccgtccgg ttgcgccacg aagtacagaa
     3241 acgggacccg ggcgggaaac agcgccgcgg cgatggagga gctgtcgggc tggctgatcg
     3301 gccccggcgg caacccgggg tgcaggtacg tgttgtacgg tgattgaatc agcaggttct
     3361 tctcgtagac ccggcgcggg cgccgcccca tcgcgtacac cacggtcgga tcggcctgca
     3421 gcagcatgcc gaggcgcagg cggttgtggt atacggcgga gacgtattcg cggtcgggcg
     3481 cgtagcgcac ttccgattcg atgatcgacg ccagcgtcac gaggtcgtgc agcgacatct
     3541 gcatgctgtc gagccgcgcg tcccaggcgg ggggccagtg gcggaggaac tcctcgatca
     3601 tcagccggac cagctcccgg ccggagaggt cgggtcggac ggtgtaggtc gtggggtaga
     3661 gatagccctc ggcggtctcg gcatgggcgc tgccgccgag cagggcgagc agctcggcat
     3721 cgcgggtggc cgcgaggaac gagtcgcgcg ggacctggag ctgtcgcgcg gcgatgtcgg
     3781 cgatctcgcc gacctgcagg ccctcgcgga cggtgaagcg gaccagaatt cctcgaccga
     3841 gttgcagggc ggcgacgatg tcggtccagc gggtgtcccg gcggaacgcg tagatgccgc
     3901 tcttcagcga gccgcccagg ccccggagcc gggcgtagag cttgaacagc acgcgggggg
     3961 cgacgatctg gtgcgccgtg agtgaatcca ccgcggccga cagcgtcgcc ccgcggggaa
     4021 tggtgaggac gacgcattcc ggcccggcgg catcgcaggg gacggagggt tgcacgcggc
     4081 aggcgctgca gagcagggcc gccacgagaa ggccgcgcgc ccggcgtctc acaccgagct
     4141 cccgcggcgg ctgtcgaggt agtgctgcag cagcagcgtg gcggcgaggg cgtccacgtc
     4201 gccggctcgg ccctcggtcc tgccgcccat ttcgtgcacg gcccggcgca cgcgcgccgt
     4261 cgtgaaccgc tcgtccacca ggtctaccgg gcggccggag cgccgggcga tgtccgccgc
     4321 cagtgcgcgg gcggcgcggg ccggctcacc ctcggcgccc gaggcctcga gcgggaggcc
     4381 gaccacgacg ccggtgacct gatgccgctc gagctgagtc aggagatcct tcatgggaaa
     4441 gcgcctgccg gtgcgccgcg tgagcgtggc gagcggctgg gcgagggtct gcgtttcatc
     4501 ggacagcgcc aggccgatgc ggcgctcgcc ccaatccacg cccagcaacc gcccggccgt
     4561 ccagccgtcc attcgtctac ccctgcgaca tcgtcgcgaa gaactcctgg ttcgacttgg
     4621 tgcgcttcat gcgctcgagc agaaactcga tcgcttccac cggcggcatg tcggcgagga
     4681 aattgcgcag caggtagacc ttgttgagct cttccttggc gagcaggagt tcttccttgc
     4741 gggtcgaggt gcgctgcacg tcgatggccg gatagacccg gcggtccgcg atgcggcggt
     4801 cgaggatcac ttccatgttg ccggtgccct tgaactcctc gaaaatgacc tcgtccatgc
     4861 gcgagccggt gtcgatcagc gccgtcgcga gaatggtcag cgatccgccg ccttcgatgt
     4921 tgcgcgccgc gccgaagaac cgcttgggtt tctgcagcgc gttcgcgtcc acgccgcccg
     4981 agaggatctt gcccgagtgc ggcacgacca cgttgtgcgc ccggcccagg cgggtgatcg
     5041 agtcgagcag gatgaccacg tccttgccgt gctcgaccat gcgcttcgcc ttttcgatga
     5101 ccatgtcggc gacctgcacg tggcggtcgg ccggctcgtc gaacgtggag gagatcacct
     5161 cggcgctctt gacgttctcc tccatatcgg tcacctcctc gggccgctcg tcgatgagca
     5221 gcacgatgag gtagatctcc ggatggtttt cgatgatcga attggcgagc ttctgcatga
     5281 ggatcgtctt gccggccttg gggggcgaga cgatgagtcc gcgctggccc ttcccgatgg
     5341 ggcagaggat gtccatcacc cgcatcgaca gatcgccgtc cttgcgctcc agccggattc
     5401 gctcctcggg gtagcgcggc cgcaggttgt cgaacgcgac ccgatgtttc gccttctcgg
     5461 gctcctcgcc gttgaccgtc tcgaccttga ggagcgccag atagcgctcc ccttccttcg
     5521 gcggtcggac ctggccgaga atcgtgtcgc ccgtgcgcag atcgaaccgc ttgatctggc
     5581 tcggg
//
LOCUS       DBNGZW010000771         7406 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1086539, whole genome shotgun sequence.
ACCESSION   DBNGZW010000771 DBNGZW010000000
VERSION     DBNGZW010000771.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 7406)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7406)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7406
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1086539"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="AC16JC_17965"
     CDS             complement(<1..446)
                     /locus_tag="AC16JC_17965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166645.1"
                     /translation="MRLARSAGFRIVAAWLAVAVFFSAENILVGAARHRPFNWQWDIY
                     HEFVYWLVWAAFTPLILGAGRRWPLGPGALPRVIAPHLGVMALLAPAQITTTYTVHWL
                     LLSAVGRPPAATLGAWLAGLGGGIVWGTLTAFLYYWLILGVHAAFV"
     gene            619..1044
                     /locus_tag="AC16JC_17970"
     CDS             619..1044
                     /locus_tag="AC16JC_17970"
                     /inference="COORDINATES: protein motif:HMM:NF024297.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR5166646.1"
                     /translation="MKSSLAVLLAAGLATAARAQGAADSAAIRATALDYIDGWYTNDA
                     ARMERALHTHLAKRLVYADSMGRSHLVDMTALELVQGTKAHPPAAVADRREDVTILSI
                     FGNTAVVRIDASTWVDFLEEIKWNGRWVIINVVWENRPR"
     gene            1110..1574
                     /locus_tag="AC16JC_17975"
     CDS             1110..1574
                     /locus_tag="AC16JC_17975"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR5166647.1"
                     /translation="MSARVQWNARNPDFERTVRDSFARQAHMATLNARITRLEPGAVD
                     LTAPFDPRFSQQNGFWHAGAVASLADSANGYAAFSLAPAGTDVLAVEFKINLLAPAEG
                     EAFRAEGRVLRPGRTLTVCRADVFGISGGDSRLVATMLSTIIIRPLTDVPAS"
     gene            1574..2167
                     /locus_tag="AC16JC_17980"
     CDS             1574..2167
                     /locus_tag="AC16JC_17980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006379485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HIR5166648.1"
                     /translation="MARRDLPVRAFRTAKAWETWLARHPDAPGLWVHLAKRSAGRQSL
                     TYREAVEGALCYGWIDGQGKRDDDKSWLLKFTPRKPNSIWSKINRARALELVAAGRMR
                     PQGLAAIERAKRNGRWAGAYDSHRTAVPPRDLAAALKKNARAGAFFATLNSQNRYAIL
                     FRIQTAKRADTRRARIERFVRMLAQHRAPYPTRRAGR"
     gene            complement(2164..2916)
                     /locus_tag="AC16JC_17985"
     CDS             complement(2164..2916)
                     /locus_tag="AC16JC_17985"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR5166649.1"
                     /translation="MTCAELETRLHAYLDAELPEAAAREVAAHVVGCPHCGPWYEEQR
                     TLRAALRDRLPAYRAPDTLRASVRRGLRVAQAVEPPRARFVASRWLATAATLVVAVGG
                     TWSLAMRAVAIRTVGDEVLAAHVRSLMPGHLTDVLSTDQHTVKPWFNGRLDFSPPVED
                     FSGAGYPLIGGRLDYVAGRSVAVLVYGRRQHMISVMLWPAGTGSSAGPAMRTVQGYHQ
                     LHWTDPGFTYWVVSDLGMTELRDLVSLLRQSH"
     gene            complement(2913..3440)
                     /locus_tag="AC16JC_17990"
     CDS             complement(2913..3440)
                     /locus_tag="AC16JC_17990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006159415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR5166650.1"
                     /translation="MDSTSLERFEAAVLPHLDSAYNLARYLTRDLHDAEDVVQEAYLR
                     ALRYFSGYRGGDARAWVLAIVRNTFHTWRRRERHQDEAMEFDEDRHSEAVEGAHPGAL
                     LDTALDWEALERALERLPTEFREVLVLRELEGMSYKEIGTVVGVPVGTVMSRLARARE
                     RLRRSLLAGTDAERA"
     gene            3582..4568
                     /locus_tag="AC16JC_17995"
     CDS             3582..4568
                     /locus_tag="AC16JC_17995"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014368896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HIR5166651.1"
                     /translation="MEQRRLGSQGLAVSALGLGCMGMSDFYTGRDDVESVATIHRTLE
                     LGITFLDTSDVYGPFTNERLVGHAIADRRDRVVLATKFGNVRSPDGAWLGVNGRPEYL
                     RTACEASLQRLGVEHIDLYYQHRVDPSVPIEETVGALADLVRAGKVRFIGLSEAAPAT
                     IRRAHATHPVTAVQMEYSLWTRDPEDGVLATCRELGIGFVAYSPLGRGFLTGRFRRAA
                     DLSADDVRTRHPRFQAANLALNLTLVEHVELLARAKRCTPAQLALAWVLAQGADIVPI
                     PGTKRRHYLEENVGALEVRLTPEELRTIDRAIPRNSASGLRYPEAAMRGINI"
     gene            4579..5550
                     /locus_tag="AC16JC_18000"
     CDS             4579..5550
                     /locus_tag="AC16JC_18000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - quinone reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase family protein"
                     /protein_id="HIR5166652.1"
                     /translation="MRAVIVSAPGGPEVLRLEEIPDLEPGPRQLVIQVEAAGVNFIDL
                     YRRAGRYKTVLPFIPGQEAAGTVTALGPGVTGFAVGDRVVCYDGGLAAYAEYVLAQVD
                     RVVRLPDDVSAEQGAAVLLQGMTAHYLASTTYPLKPGDTCLVHAAAGGTGLLLCQIAR
                     KRGARVIGTVSTAAKEALAREAGAHEVIRYTETDFVAAVRGLTDGAGVQVIYDSVGRT
                     TFEAGLRCLVRRGTMVLFGQSSGPVEPFDPQVLNERGSLFLTRPTLAHYVETRAELEQ
                     RAGELLGWLRDGTLTVRIDRTVPLAQAAEAHRALESRGTSGKILLRP"
     gene            complement(5547..6059)
                     /locus_tag="AC16JC_18005"
     CDS             complement(5547..6059)
                     /locus_tag="AC16JC_18005"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5166653.1"
                     /translation="MSEVESGSPVVRAATPADAPELARLRYEFRAAERLAVEPSEEFV
                     ARTTEWMRERLAEGTAWRCMVAVQNGAIVGQVWVTLIEKVPNPGSGEPERHAYLTNLY
                     VRPAERGGTGSALLEAALAWCRGQRVDTVILWPSERSRTLYARYGFDASRAILALPLA
                     PDPHGRRPEP"
     gene            6107..6415
                     /locus_tag="AC16JC_18010"
     CDS             6107..6415
                     /locus_tag="AC16JC_18010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HIR5166654.1"
                     /translation="MIARLWHGWTTRENADAYEALLRSEVLPGIHGIPGYHGAYLLRR
                     NADPEVEFVTLTLFESWEAVRAFAGPDFEAAVVPPAARKLLARFDARSVHYDILQTPA
                     "
     gene            6467..>7406
                     /locus_tag="AC16JC_18015"
     CDS             6467..>7406
                     /locus_tag="AC16JC_18015"
                     /inference="COORDINATES: protein motif:HMM:NF019335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HIR5166655.1"
                     /translation="MKSFVGHGAVFLTGLVAIGAGRAAAVPCVAADTVRGAVRDSAGH
                     PLPSVEVLLTDLARRTTTGADGAFAFTAVPNGAYTLVFRRAGYGPLARHIDVTGSVEV
                     QVVLRASAVQLDAVTVTATRAPADPLSSPLPTATLGAAELAREYRVSLAHALESVAGV
                     RALTTGGEIGKPVIRGLTGARVLVLGDGNRLEDYSWSDEDGPSVETRLADRVEVIRGP
                     ASVLYGSDALGGVVNVIPEDVPDAQRHVPLRGGVDAYFATNNHELGSALHASGATGGV
                     GFLAGLVGRHSEALHTPVAELENTGFTALSGEAAVGT"
BASE COUNT         1121 a         2586 c         2602 g         1097 t
ORIGIN      
        1 tagacgaagg ccgcgtgcac gcccaggatc agccagtaat agaggaacgc cgtgagcgtg
       61 ccccagacga tcccaccccc gaggcccgcg agccacgcgc cgagcgtggc ggctggcgga
      121 cgaccgacgg cgctcagcag cagccagtgc accgtgtacg tggtggtgat ctgcgccgga
      181 gcgagcagcg ccatgacgcc caggtgcggg gcgatgaccc gggggagcgc gcctgggccc
      241 agcggccagc ggcgcccggc gccgaggatg agcggcgtga acgcggccca cacgagccag
      301 tagacgaact cgtggtagat gtcccattgc cagttgaaag ggcggtgccg cgcggcgccg
      361 acgaggatgt tctccgcgct gaagaacacg gcgacggcga gccacgccgc aacgatcctg
      421 aagccggccg aacgcgccaa tctcatgacg caatcttcac cgcgcgagag gtcgcgggca
      481 acgggccgcg tcgaagcgga ccactcgtca cgctgtgagc cgcgttcgtc cctgcgccgg
      541 ccgcgccggc cccctcgtcc ttggcgcgac gcgacgcacc tcccaacttg cggcccgacc
      601 cacactcccg aggatcgcgt gaagagttcg ctcgcagtgc tgctcgcggc cggtctcgcc
      661 acggcggccc gcgcccaggg cgccgccgac tcggcggcga tccgcgccac cgcgctcgac
      721 tacatcgacg gctggtacac caacgacgcc gcccgcatgg agcgggcgct ccacacgcac
      781 ctcgccaagc gcctggtgta cgcggattcg atgggccgca gccacctggt ggacatgacc
      841 gcgctcgagc tggtgcaggg gacaaaggcg cacccgccgg cggcggtcgc ggaccggcgc
      901 gaggacgtga caattctcag catcttcggc aataccgccg tcgtgcgcat cgatgcctcg
      961 acctgggtgg attttctgga agagatcaaa tggaacgggc gctgggtgat catcaacgtc
     1021 gtctgggaaa atcgcccgcg gtgagcgggg ggcggtagcg cgtctcctct tgtgcggagg
     1081 gagcagcggg ggatcttcac agcaccaccg tgagcgctcg agtgcagtgg aacgcccgca
     1141 atccagactt cgagagaacc gtccgcgaca gtttcgcgcg gcaggcgcac atggccacgc
     1201 tgaacgcccg gatcacccgt ctcgagccgg gagcggtcga cctgaccgct ccattcgatc
     1261 cccggttcag ccagcagaac ggcttctggc acgcgggcgc ggtggccagt ctcgccgact
     1321 cggccaacgg ctatgcggcg ttctcgctcg cgcctgccgg gaccgacgtc ctcgcggtcg
     1381 agttcaagat caatctgctc gcgccggccg agggggaagc ctttcgcgcc gagggtcgcg
     1441 tgctgcgccc gggccgcacg ctgacggtct gcagggccga cgtgttcggg atctcagggg
     1501 gtgacagccg gctcgtcgcc acgatgctgt cgacgatcat catccggcct ctgacggacg
     1561 ttcccgcgag ctgatggcgc gacgcgacct gcccgtccgc gcgttcagga ccgccaaggc
     1621 gtgggaaacg tggctggccc gccaccccga cgcgcccggt ctctgggtgc acctggccaa
     1681 gcgttcagct ggccggcagt ctctcaccta ccgggaggcc gtggaagggg cattgtgcta
     1741 cggctggatc gacggccagg ggaaacgcga cgacgacaaa tcgtggttgc tgaagttcac
     1801 acccaggaag ccgaacagca tctggtccaa gatcaatcgc gcccgggcac tcgagctggt
     1861 cgcggcggga cgcatgcggc cgcaggggct cgccgcgatc gagcgggcca aacgcaacgg
     1921 gcgctgggcc ggggcgtacg attcgcaccg cacggcggtc ccgcctcgcg atctcgccgc
     1981 cgccttgaag aagaacgcca gggccggggc gttcttcgcc acgttgaaca gccagaaccg
     2041 gtacgcgatc ctgttcagga tccagacggc gaaacgggcc gacacgcgtc gcgcgcgcat
     2101 cgagcggttc gtccggatgc tcgcgcagca ccgggcgcca tatccgactc gaagggcggg
     2161 acgctagtgt gactggcgca acagcgaaac gagatctctg agctcggtca tgccgagatc
     2221 cgacacgacc cagtaggtga agccgggatc cgtccagtgg agctggtgat agccctgaac
     2281 ggtccgcatc gcgggtccgg cgctcgagcc ggtgcccgcc ggccagagca tcacgctgat
     2341 catgtgctgg cgccggccgt agacgagaac cgcgacggag cgcccggcga cgtagtcgag
     2401 ccggccgccg atcagggggt agccggcccc ggagaagtcc tcgaccggcg gcgagaagtc
     2461 gaggcgtccg ttgaaccacg gtttcacggt gtgctggtcg gtcgagagca cgtccgtgag
     2521 atgccccggc atgagcgaac gcacgtgggc ggccagtacc tcgtcgccga cggtgcggat
     2581 cgccacggcg cgcatggcga gcgaccaggt gccgccgacg gcgactacca gcgtcgccgc
     2641 cgtggcgagc cagcgcgacg ccacgaagcg cgcgcgcgga ggctcgacgg cctgcgccac
     2701 ccgcaggccg cggcgcaccg acgcccgcag ggtgtcggga gcccggtagg cggggagccg
     2761 gtcgcgcagt gcggcgcgca gcgtccgctg ctcctcgtac cacggaccgc agtgggggca
     2821 tccgacgacg tgcgccgcca cctcccgggc tgccgcttcc ggaagctcgg catccaggta
     2881 cgcgtgcagg cgcgtctcga gctcggcgca ggtcatgcgc gctccgcgtc cgttccggcg
     2941 agcagcgacc ggcgcagccg ttcccgggcc cgcgccagcc gcgacatcac ggtgccgacg
     3001 ggcacgccca cgacggtgcc gatttccttg tacgacatcc cttccagctc gcgcagcacc
     3061 agcacctccc ggaactccgt gggcagccgc tcgagcgccc gctcgagtgc ttcccagtcc
     3121 agcgcggtgt cgagcagggc gccgggatgc gcgccttcga ccgcctcgct gtgccggtct
     3181 tcgtcgaact ccatcgcctc gtcctggtgt cgctcgcgcc gccgccacgt gtggaacgtg
     3241 ttgcgtacga tggcgaggac ccaggcgcgc gcgtccccgc cgcggtaccc gctgaagtag
     3301 cgcagggcac gcagatacgc ctcctgcacc acgtcttcgg cgtcgtgcag gtcgcgcgtc
     3361 aggtagcgcg ccaggttgta ggccgaatcc aggtgcggca gcaccgcggc ttcgaaccgt
     3421 tcgagactcg tggaatccac cgtccctcga gaagcgagac tcgctccggc cgcggataat
     3481 tccccgtggc cctggccggc cgtcgtacac cggaaccggg cgccggaatg tcaggtttcg
     3541 gggtagactt ggagcgtaac cctgatggag ggagtggagc gatggagcag cgacgactgg
     3601 gatcccaggg acttgccgtg tcggccctcg gactgggctg catgggaatg tcggatttct
     3661 atacagggcg cgacgacgtc gaatcggtcg ccacgatcca tcgtaccctg gagctcggca
     3721 tcacctttct cgatacctcc gacgtctacg gaccgttcac caacgagcgt ctcgtgggac
     3781 acgccatcgc ggaccggcgc gaccgggtcg tgttggcgac caaattcggc aacgttcgca
     3841 gtcctgacgg cgcctggctg ggggtcaacg gccgtcccga gtacctccgt accgcctgtg
     3901 aggcgtcgct ccagcgcctc ggggtcgagc acatcgacct ctactaccag caccgcgtgg
     3961 acccctcggt gccgatcgag gagacggtcg gggccctggc ggacctcgtg cgcgcgggca
     4021 aggtccgctt catcggcctc tcggaggccg cacccgccac gatccgccgc gcccatgcta
     4081 cccatcccgt gacggcggtg cagatggagt actcgctctg gacccgtgac cccgaagacg
     4141 gcgtgctcgc gacgtgccgc gagctgggca tcgggttcgt cgcctacagc ccgctggggc
     4201 gcgggttcct gaccggcaga ttccgccgcg cggctgacct ttcggcggat gacgtccgca
     4261 cccggcaccc acgatttcag gcagccaatc tcgccctgaa cttgacgctt gtggagcacg
     4321 tcgagctgct cgcgcgcgcg aaacggtgca cgcccgccca gctggcgctc gcctgggtgc
     4381 tggcccaggg ggcggacatc gtcccgattc ccggcaccaa acggcgccac tatctcgagg
     4441 agaacgtggg cgccctggaa gtgcggctca cgccggaaga gttgcgcacc atcgatcgcg
     4501 cgattcccag aaactccgcc tcgggactcc gctaccccga ggccgccatg cgcggcatca
     4561 acatctgacg ggccgccggt gagagccgtc atcgtctccg ctccgggcgg tcccgaagtt
     4621 ctgcgtctcg aggaaatccc ggacctggag cccggaccgc gtcagctcgt gatacaggtc
     4681 gaagccgccg gcgtcaactt catcgatctg tatcggcgcg ccgggcgata caagaccgtg
     4741 ctgccgttca ttccgggcca ggaggccgcg gggacggtca cggcgctggg ccccggggtc
     4801 accgggtttg cggtggggga ccgcgtggtc tgctacgacg gtggactggc cgcgtacgcc
     4861 gagtacgtgc tggcccaggt cgaccgcgtg gtgcggctcc ccgatgacgt gagcgcggag
     4921 cagggcgccg cggtgctgct ccaggggatg accgcgcatt acctcgccag cacgacgtat
     4981 cctctcaaac ccggcgacac gtgcctcgtg catgcggccg ccggtgggac cgggctgctg
     5041 ctgtgccaga tcgccaggaa acgcggggcg cgggtgatcg gcaccgtgtc cacggccgcc
     5101 aaggaagcgc tggcgcgcga ggccggtgcc cacgaagtga ttcgttacac cgagacggat
     5161 ttcgtggcgg cggtgcgagg cctgacggat ggagcgggag tgcaggtgat ctacgattcc
     5221 gtgggccgca cgacgttcga ggcgggactg cgctgcctgg tacggcgggg cacgatggtg
     5281 ctgttcgggc agtcgagcgg gcccgtcgag ccgttcgatc cacaagtcct gaacgagcgc
     5341 gggtccctgt tcctcacccg tcctacattg gctcactacg tcgagacccg tgccgagctg
     5401 gagcagcggg ccggtgagct gctgggctgg ttgcgggacg ggaccctgac cgtgcgtatc
     5461 gatcgcaccg tgccgctggc acaggccgcc gaggcgcatc gcgccctgga aagccggggg
     5521 acgtcgggga agatcctgct gcggccctaa ggctccggtc ggcggccgtg cgggtcgggt
     5581 gcgagcggca gcgccaggat ggcccgggac gcgtcgaagc cgtagcgcgc gtacagcgtg
     5641 cggctgcgct cgctgggcca caggatcacg gtatccaccc gctggccgcg acaccaggcg
     5701 agcgccgcct cgagcagcgc gctcccggta ccccctcgct cggccggacg cacgtacagg
     5761 ttcgtgaggt aggcgtgccg ctccggctca ccgctgccgg ggttggggac tttctcgatc
     5821 agggtcaccc acacttgacc cacgatcgcc ccgttctgca cggcgaccat acagcgccat
     5881 gccgtgccct cggccaggcg ctcccgcatc cattccgtgg tccgcgcgac gaactcctcg
     5941 ctcggctcga ccgcgaggcg ctcggccgcg cgaaactcgt agcgcagccg ggccaactcg
     6001 ggcgcatcgg cgggcgtggc cgcgcgcacc accggcgacc ccgactccac ttcggacata
     6061 cgggcacgat accggcccgc gtgactctcg actagtcttg atgccaatga tcgcccgcct
     6121 gtggcacggg tggaccaccc gcgagaacgc cgatgcctac gaggcgctgc tgcgcagcga
     6181 ggtcctcccc ggcatccacg gcattcccgg ctatcatggc gcttacctgc tgcggcgcaa
     6241 cgccgatccg gaagtggagt tcgtcaccct gaccctgttc gagtcgtggg aggccgtccg
     6301 cgccttcgcc ggacccgatt tcgaggcggc ggtggtcccc ccggcggctc gaaagctgct
     6361 ggcccggttc gacgcgcggt cggtgcacta cgacatcttg cagacccccg cctgacaggg
     6421 cccccatctt gcgcccgttt cttcgggcgc gtatttgctt tgacccatga aatcgttcgt
     6481 cgggcacggg gccgtcttcc tgaccgggct ggtcgccatt ggcgcggggc gggcggcggc
     6541 cgtgccgtgc gtagcggccg ataccgtccg cggcgccgtc cgggattccg ccggccatcc
     6601 cctgccgtcg gtggaggtgt tgctcaccga cctggcgcgc cgcaccacca ccggagccga
     6661 cggcgcgttc gcgttcaccg ccgtgccgaa tggcgcctat acgctggtgt tccgccgagc
     6721 gggctacggt ccgctggcgc gccacatcga cgtcacggga tccgtggaag tccaggtcgt
     6781 gctgcgcgcg tcggccgtcc agctcgacgc ggtcaccgtc accgcgaccc gtgctccggc
     6841 cgatccgctg tcttcgccgc ttcccacggc gacgctcggt gcggcagagc tggcgcgcga
     6901 gtaccgggtc tccctcgccc atgcgctgga gtcggtggcg ggggtccgcg cgctcacgac
     6961 cggcggcgag atcggcaagc cggtgatccg cgggctcacg ggagcgcggg tgctggtgct
     7021 gggtgacggc aaccggctcg aagattattc gtggagcgac gaagacggcc cctcggtcga
     7081 aacccgactt gccgaccggg tcgaggtgat tcgcggtccg gccagcgtcc tgtatggctc
     7141 ggacgccctg ggcggggtgg tgaacgtcat ccctgaggac gtgcccgacg cccaacggca
     7201 cgtccccttg cggggcgggg tggacgcata cttcgccacc aacaatcacg agctggggag
     7261 cgcgctgcac gcctcgggcg ccacgggtgg tgtggggttt ctcgcgggac tggtcggccg
     7321 ccactccgag gcgctgcaca cgccggtggc ggagctcgag aacacggggt tcacggcatt
     7381 gtcgggcgaa gccgcggtgg gcacgc
//
LOCUS       DBNGZW010000772         5850 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1768979, whole genome shotgun sequence.
ACCESSION   DBNGZW010000772 DBNGZW010000000
VERSION     DBNGZW010000772.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5850)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5850)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5850
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1768979"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1349
                     /locus_tag="AC16JC_18020"
     CDS             93..1349
                     /locus_tag="AC16JC_18020"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011689312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SufS family cysteine desulfurase"
                     /protein_id="HIR5166656.1"
                     /translation="MTTVSRAPVHGPAAFDAARLRADFPILSTTARGRPLVYLDNAAT
                     SQKPRAVIDRLERYYTTENANVHRGVYDLSERATAEYERAREKVRVFLNAASTREIVF
                     VRGTTEGINLVASSWGRSTLQPGDEVLVSAMEHHSDIVPWQLVCEERGAVVRVIPMNR
                     RGELLLDEYEQLLTGRTRIVAVAHVSNSLGTVNPIADIVRLAHARGVPVLVDGAQAAP
                     HLPIDVQALGADFYTVSGHKMFGPTGIGVLYGKADRLDAMPPYQGGGDMIKAVSFAKT
                     TYADLPAKFEAGTPHIAGAIGLGAAIDYLGALDWDAVAAHERDLLGYASTRLNELPGI
                     RLVGTAAHKAAVVSFVVDGVHAHDVGTIVDQEGVAIRTGHHCTQPVMDFFGVPATARA
                     SFALYNVRADVDALVAALEKVRKVFS"
     gene            1349..1801
                     /gene="sufU"
                     /locus_tag="AC16JC_18025"
     CDS             1349..1801
                     /gene="sufU"
                     /locus_tag="AC16JC_18025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018002626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIR5166657.1"
                     /translation="MGDLSDLYQQIIVEHNRSPRNFKTLPHPTNAAEGSNPLCGDQIR
                     VELQLDGDRIADIGFQGSGCAISQASASLLTGAVLGKTKSEAEALFREIHTMLTSATD
                     APVDAAKLGKLAALAGVRRFPVRVKCASLSWHTLHAALREGAEPVTTE"
     gene            1854..2819
                     /locus_tag="AC16JC_18030"
     CDS             1854..2819
                     /locus_tag="AC16JC_18030"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR5166658.1"
                     /translation="MTTPFLVLDHITKRFAGHTAVDDLSLTVPAGAIYGLLGPNGAGK
                     TTSIRMAMNILVPDEGRVRLFGGEGTARDYSDRIGFLPEERGLYPKMRVLDVLVFLAE
                     AKGVDRRAARSRALDWLERLGLGDWRMRKVSDLSKGMQQKVQFISTLLHDPDLVLLDE
                     PFSGLDPVNTEVMREIVIDLRRRGKTILFSTHIMEHAEKLCDYVCIIAKGRKLVDDTL
                     GRVKETHGGRHVTVAFDGDQGSAQQVFADRRLVGRAEDFGQYAELELSPGADAQEVLR
                     ALVASGARLSRFELTAPSLHKIFIDLVGPEAVRAEAVTNGRSHHA"
     gene            2812..4077
                     /locus_tag="AC16JC_18035"
     CDS             2812..4077
                     /locus_tag="AC16JC_18035"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR5166659.1"
                     /translation="MRKVLAVVRREFVERVRTRWFWISALLGPIFFAALIFFPILFSG
                     GGRSKRLVVVDGTQSAFGANVADSLNTSGAFTLVERVPPRAGLLDSLTGEVSRRRLDG
                     FLIVTDATVDSGVAEYRSSNVSSLRDIGVLKGVLGRMAVNARLERAGVDPQVVRAAQL
                     QVDLDTRKISGSTTTGESSAQSFTLAYVMAILLYMAILLYGVNVMSSVLEEKTTHVVE
                     VLVSSLRPFQLMVGKIVGVGAVSLFQFAIWAVSAVLLFSQRAALTAGMTSAPGSDRTI
                     FEVPQVSLGTAAVFGAYFIGGFLLYSAMFAAVGAMSSSESEARQAQQPVVFLLLIAYL
                     SIWALSGNPDSTYALTISFIPFTSAIAMPVRWAASIVPTSQLLLSLGLLVLGIVAVSW
                     VAARIYRVGILMTGKRPRFRDLIRWVRTA"
     gene            4565..4759
                     /locus_tag="AC16JC_18040"
     CDS             4565..4759
                     /locus_tag="AC16JC_18040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013121637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HIR5166660.1"
                     /translation="MRQMLKRLWNDESGQGMVEYALIVALVAIGLIAVFLFLRNSTGD
                     VLNTAADSLQNAPAASYATH"
     gene            4842..5363
                     /locus_tag="AC16JC_18045"
     CDS             4842..5363
                     /locus_tag="AC16JC_18045"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:NF013632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A24 family peptidase"
                     /protein_id="HIR5166661.1"
                     /translation="MTVLMTLALVTLLVIAALFDLRERRIPNAVTLSGVAVALTIRLV
                     WGWGSVLEGGTGVIFAVLVGLAPFTLGLLGGGDVKLLGAVGAFVGMHRLFGALLVVAV
                     VGGVMAMLEATRQRAFMRALVGVGRFARQLVWFGPAGITSTIESPEAMTVPYGVAIAV
                     GSLLWWFFGGASL"
     gene            5360..5770
                     /locus_tag="AC16JC_18050"
     CDS             5360..5770
                     /locus_tag="AC16JC_18050"
                     /inference="COORDINATES: protein motif:HMM:NF019428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HIR5166662.1"
                     /translation="MKRLRNNVRGQAMVEFAIVLPLLLILLIAVFEFGRAWNVYHAVT
                     DAARLGARSAVVADPVTTQDSVYAIIRRALSRAAIDTNNATISMTGWRTGSGTPASIN
                     IQVPYQFVFLKPFMHWSNNEASITLQSTFVMRNE"
BASE COUNT          885 a         1895 c         2003 g         1067 t
ORIGIN      
        1 ctggaccgcg tggcgattgc gggcgcgcga gaccggctcg accggcagat ccggcatcgc
       61 ctgggcgtcc ccgatccgga gacgggtgcg gcgtgacgac cgtcagccgg gcgccggtcc
      121 atggacccgc cgccttcgac gcggcacggc tgcgcgccga cttcccgatc ctctccacca
      181 ccgcccgcgg ccggccgctc gtctacctcg acaacgccgc gaccagtcag aaaccccgcg
      241 cggtgatcga tcgcctcgag cgctactaca cgacggagaa cgccaacgtc caccgcggcg
      301 tctacgacct gagcgagcgc gcgaccgccg agtacgagcg ggcgcgggaa aaggtgcggg
      361 tgtttttgaa tgccgcttcg acccgcgaga tcgtgttcgt gcgcggcacc accgagggga
      421 tcaacctcgt tgcctcgagc tggggtcgat ccaccctcca gccgggcgac gaagttctgg
      481 tatcagcgat ggagcaccat tccgacatcg tcccctggca gctcgtgtgc gaggagcggg
      541 gggccgtggt ccgcgtgatc ccgatgaacc gccgcggcga gctgctgctc gacgagtacg
      601 aacaactgct caccggccgc acgcggatcg tcgccgtcgc gcacgtgtcc aactccctgg
      661 ggacggtgaa ccccatcgcc gacatcgtgc gcctcgccca tgcgcgtggc gtccccgtcc
      721 tggtggacgg cgcgcaggcg gcgccgcacc tcccgatcga cgtgcaggcg ctgggggccg
      781 acttctacac ggtgtcggga cacaagatgt tcgggcccac cggcatcggc gtgctgtacg
      841 gcaaggcgga ccggctcgac gcgatgccgc cctatcaggg cggcggcgac atgatcaagg
      901 ccgtgagctt cgcgaagacc acctatgccg accttcccgc gaagttcgag gccgggacgc
      961 cccacatcgc cggcgccatc ggattgggcg ccgccatcga ctacctcggc gcgctggatt
     1021 gggatgccgt ggccgcgcac gaacgggatc tgctggggta cgcgagcacc cgcctgaacg
     1081 aacttccggg catccggctc gtcggcaccg ccgcgcacaa ggcggctgtc gtctccttcg
     1141 tggtggacgg cgtgcacgcg cacgacgtgg gcaccatcgt ggaccaggaa ggcgtcgcga
     1201 tccgcaccgg gcatcactgt acccagccgg tgatggactt cttcggcgtt ccggcgaccg
     1261 cccgggcctc gttcgcgctc tacaacgtgc gcgccgatgt ggatgccctc gtcgcggcgc
     1321 tcgagaaggt ccggaaggtg ttctcctgat gggcgacctg agcgatctct accagcagat
     1381 catcgtcgag cacaaccgga gcccgcggaa cttcaagaca ctgccccacc ccaccaacgc
     1441 cgcggaaggc tcgaatccgc tgtgcgggga ccagatccgg gtcgagctgc agctcgacgg
     1501 cgaccgcatc gccgacatcg gattccaggg atcggggtgc gcgatctcac aagcgtccgc
     1561 ttccctcctc accggcgcag tcctggggaa gacaaaatcg gaagccgaag cgctgtttcg
     1621 ggagattcac acgatgctga cgtcggccac cgacgccccc gtcgacgcgg ccaagctcgg
     1681 gaaactggcg gcgctggccg gtgtgcggcg ctttcccgtc cgcgtgaagt gcgccagcct
     1741 atcctggcat accctccacg ccgcgttgcg ggaaggggcg gagcccgtca cgaccgagta
     1801 gcttatcccc cgctagaatc ccggccggtc agtgtccgtc ttccgcgggg cgtatgacca
     1861 caccgtttct cgttctcgac cacatcacca agcggttcgc cgggcacacc gccgtcgacg
     1921 acctgtcgct gacggtcccc gccggggcca tctacggcct gctcgggccg aacggcgccg
     1981 ggaagaccac ctccatccgg atggcgatga acatcctcgt tcccgacgaa gggcgcgtcc
     2041 ggctgttcgg cggggagggg acggcgcggg attactccga tcgcatcggg ttcttgccgg
     2101 aagagcgcgg cctgtatccc aagatgcggg tgctcgacgt cctggtcttt ctggccgaag
     2161 cgaagggtgt ggaccgccgc gcggcccggt ccagggcgct ggactggctc gagcgcctcg
     2221 ggctcgggga ttggcgcatg cggaaggtct cggacctctc caaggggatg cagcagaagg
     2281 tgcagttcat ctcgaccctg ttacacgacc ccgacctcgt gctgctggac gaaccgttct
     2341 ccgggctgga tcccgtgaac accgaagtga tgcgggaaat cgtgatcgac ctgcgtcgcc
     2401 gcgggaagac gatcctgttc tcgacccaca tcatggaaca cgccgagaag ttgtgcgact
     2461 acgtctgcat catcgccaag gggaggaagc tggtggacga tacgctcggc cgggtgaagg
     2521 aaacccacgg gggccggcac gtcacggtgg cgttcgacgg cgaccagggg agcgcccagc
     2581 aggtgttcgc cgatcgccgc ctggtcggcc gggcggagga tttcggccaa tacgcggagc
     2641 tcgagctgag tccgggcgcc gatgcgcagg aggtgctgcg ggcgctcgtc gcctcgggag
     2701 ctcggctgtc acggttcgag ctcaccgcgc cttcgctcca caagatcttc atcgacctgg
     2761 ttggtcccga ggcggtccgg gcggaagcgg tcacgaacgg acggtcccat catgcgtaag
     2821 gtgctcgccg tcgtgcgccg ggagttcgtg gaacgcgtgc ggacccggtg gttctggatc
     2881 tcggcactgc tcggaccgat cttcttcgcc gcgctgatct tcttcccgat cctcttctcc
     2941 ggcggcggcc ggtccaaacg gctcgtggtg gtggacggga cgcagtcggc gttcggcgcg
     3001 aacgtcgcgg attcgctcaa caccagtggc gccttcacgc tcgtcgagcg tgtgccgccg
     3061 cgggcggggt tgctggactc cctgacgggg gaagtgagcc gccgccggct cgacggcttt
     3121 ctgatcgtga cggatgcgac cgtggattcc ggcgtggccg agtaccgcag ctccaacgtc
     3181 tcctccctgc gggacatcgg ggtgctgaag ggcgtgctcg ggaggatggc ggttaacgcg
     3241 cgcctggagc gcgccggcgt ggacccacag gtcgtgcggg ccgcgcagct gcaggtcgat
     3301 ctcgacacgc ggaagatctc gggctcgacg accactggag aaagctccgc ccagtcgttc
     3361 accctcgcgt acgtcatggc catcctgctc tacatggcga tcctgctgta cggcgtgaac
     3421 gtgatgagct cggtgctgga agagaagacg acgcatgtcg tcgaagtgct cgtgtcctcg
     3481 ctgcggcctt tccagctgat ggtcggcaag atcgtgggcg tcggtgccgt ctcgctgttc
     3541 cagttcgcga tctgggccgt atcggcggtg ctgttgttct cgcagcgggc ggcgctgacg
     3601 gccgggatga cttccgcccc ggggagcgat cgcaccatat tcgaggtgcc gcaggtgtcg
     3661 ctggggaccg cagcggtttt tggcgcgtac ttcatcggcg ggtttctgct ctactcggcc
     3721 atgttcgccg cggtgggggc catgtcgtcg agcgagtcgg aggcccggca ggcacagcag
     3781 ccggtggtct tcctgttgct gatcgcgtat ctctcgattt gggcgttgtc gggcaacccc
     3841 gactcgacct acgcgctgac gatctcgttc attcccttca cgtcggccat cgccatgccg
     3901 gtccgatggg cggccagcat cgtgcccacg agccagctcc tcctgtcgct ggggctcctg
     3961 gtgcttggca ttgtcgccgt cagctgggtc gcggcccgga tctatcgggt cgggatcctc
     4021 atgacgggga aacgcccacg tttccgggac ctcatacgct gggtccgcac cgcatagcgg
     4081 cacgcttccc acccccgatg ttaccgtggc gccctgagaa cactctttag cggggtttga
     4141 gaacgacctg aagagttccc cggcggcagt tgcgatcgat ctccagattg caactgcttt
     4201 cggcacagca agttgcgctg gaacgcccgg gttggttggc gacaccctgc ttacgcaaca
     4261 ctgtagcgag tgcacagcaa gttgttgctg ctgcacagca cactgtggcg taggggcagt
     4321 ggcgtttgca ggcaggtttt ccccgccatt aggctcaccc gctcgccgtc gtcgattttt
     4381 gttaggcaat cccccgtcgc tccttgtgtt tgcgcgtcgg tgtgacgccg gacacacagt
     4441 tctgtagctc gcccggtaca cgtgttgcgt tggggcacgg cgcggctggc atgtgccagc
     4501 tgcccgctcg tgccgagtca cgggcgcaac tacgcaaggg cccgttggcc cgggaggatt
     4561 tccgatgcgg cagatgctga agcggttgtg gaacgacgaa tccgggcagg gcatggtcga
     4621 atacgccctg atcgtcgcgc tggtggcgat cggtctgatc gccgtgttcc tgttcctgcg
     4681 caactcgacc ggggacgtcc tcaatacggc ggccgactcg ctgcagaacg cgcccgcggc
     4741 ctcctacgcc acccactagt ggcgatgtcg agcgaccgag gtcagggact gggtgtcagt
     4801 cccgttccct ggcctcgggc tcgaccgaga ggcacacgct aatgacggtt ctcatgacct
     4861 tggccctggt cacgctgctc gtgatcgccg ccctctttga cttgagggag cggcgcatcc
     4921 ccaacgctgt gacgctgtcc ggcgttgcag tggcgctgac catccgcctc gtgtgggggt
     4981 ggggctcggt cctggagggg ggcacgggag tgatcttcgc cgtgctggtg ggactcgcgc
     5041 cgttcacgct ggggctgctg gggggtggcg acgtgaagtt gttgggagcg gtgggtgctt
     5101 ttgtgggcat gcacaggctc ttcggagctc ttctcgtggt ggcggtggtc ggaggcgtga
     5161 tggccatgct cgaggcgacg cggcagcggg cgttcatgcg ggcactcgtg ggtgtcggcc
     5221 gcttcgcgcg gcaactggta tggttcggcc cggcggggat tacctcgacg atcgaatcgc
     5281 ccgaggcgat gacagtccct tacggagtgg cgatcgctgt gggatcgctc ctttggtggt
     5341 tctttggagg ggccagccta tgaaacgcct ccggaacaac gtgcgcgggc aggcgatggt
     5401 cgaatttgcg atcgtgctgc cgctcctgct gatcctgttg atcgcggtgt tcgaattcgg
     5461 gcgcgcctgg aacgtgtacc acgccgtgac cgacgcggct cggctcgggg cgcgcagcgc
     5521 ggtcgtggcc gatccggtga cgacgcagga ctcggtgtac gcgatcatcc ggcgggcgct
     5581 gtcgcgggcc gccatcgata cgaacaacgc caccatctcg atgacggggt ggcggaccgg
     5641 gtcgggaacc ccggcatcga tcaacatcca ggttccctat cagttcgtgt ttctgaagcc
     5701 gttcatgcat tggtcgaaca acgaggcgag catcacgctc caatcgacgt tcgtgatgcg
     5761 caatgaataa cgccacatac gaatcgtgga ggaggtcgcg ttgagacaac gccggctttg
     5821 gctcatcctg tcgcttgcgc tgctgtccgg
//
LOCUS       DBNGZW010000773         4101 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_474128, whole genome shotgun sequence.
ACCESSION   DBNGZW010000773 DBNGZW010000000
VERSION     DBNGZW010000773.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4101)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_474128"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..632
                     /locus_tag="AC16JC_18055"
     CDS             <1..632
                     /locus_tag="AC16JC_18055"
                     /inference="COORDINATES: protein motif:HMM:NF038353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FxLYD domain-containing protein"
                     /protein_id="HIR5166663.1"
                     /translation="LAGIYAQAGRRAEAAAVFDDLLAHSSDLTTSDLYDLGQRLLQAK
                     LLGPGTKAYTLALQQNPYHRNALAELASAYIDARDTANALATAQRLQALDPLNKAALH
                     IVAQAWELRGRRDSAQVYGTLADTLSLDISIASLVGDSAGVTVTGVGSNLGNGPSKAL
                     RITVDLLDARGTAVASQAVEVPALQPGGSHQFQVKGAGRGIVGWRYRAG"
     gene            799..2100
                     /locus_tag="AC16JC_18060"
     CDS             799..2100
                     /locus_tag="AC16JC_18060"
                     /inference="COORDINATES: protein motif:HMM:NF016354.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF512 domain-containing protein"
                     /protein_id="HIR5166664.1"
                     /translation="MNDRRLEDFLDWEFLTADEAFVLRARLPSGQELECDIERPEGMP
                     LGVALEPPRIRRCANHCDFCFVDGNPAGLRSSLYIRADDYRLSFRYGNFATLTNLKPW
                     DEARIIEYRLSPLYVSVHATDPVVRRWLLRNPEAPDIVTQLSDFAAHGIRFHTQVVLV
                     PGVNDGAALERTLQDLYELGAAVLSVSIVPVALTEYSKHSLVRAISPDDCREALATVD
                     RWATRAVAERGHPWCFGADELYLVAGAALPPAAWYGEFEQRENGVGAVRYLQTRIVEA
                     SGTYGDWTGKRIGIATGTAMAPLMPQVIETLMKETGGRFEVLALENHLFGASVTTAGL
                     LPGAAFLTALRERRDLDLALLPAEAVNDDLRFMDDMDAHDLAAAVPMEIRFSYDFADV
                     LSERGMRNDECGIEPSDSVRQEPRPSNSTFDIPHSAWWEGP"
     gene            2097..3425
                     /gene="der"
                     /locus_tag="AC16JC_18065"
     CDS             2097..3425
                     /gene="der"
                     /locus_tag="AC16JC_18065"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HIR5166665.1"
                     /translation="MKGAPTVAIIGRPNVGKSTLFNRLIGRREAIVDAQPGVTRDRNF
                     AAADWNGRRFWLVDTGGLVPGASDALNRAIRAQVEAAVAESDVLLFLVDVEEGVHPAD
                     LEIAQYLRGVDRPVLLVANKADRLPDDQRHLAFYELGLGDPLPVSAAIGKSSGDLLDR
                     IVEMLPTREGGDDESAVQVAIVGRPNVGKSSLLNRLLGRDRMVVADEPGTTRDAVDTP
                     VEHEGRRLVFIDTAGLRKRSKVQDDVEFYATLRTSRAIERADVCVLVVDALDGMHAQD
                     LKIIGDAWERGAGVIVAVNKWDLIEEKETNTAVRGERELVAKAPFLRFIPFLYVSAKT
                     GQRVSKLFDLILTVAEERTKRVPTAEVNRVLESLVSRQQPPQPVGESVRLFYASQIGT
                     APPRFAIVANRPDAIPESYTRYLVNGFREAWAFTGAPVNVKFRRKRQERR"
     gene            3422..4027
                     /gene="plsY"
                     /locus_tag="AC16JC_18070"
     CDS             3422..4027
                     /gene="plsY"
                     /locus_tag="AC16JC_18070"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR5166666.1"
                     /translation="MTIALWLAAAYLLGATPTAYLAGRLARGIDLREHGSRNLGATNV
                     YRVLGWRWAIPVALLDIAKGAVPVVLFARWAGMGPWGAVALGIAAVLGHVFSPYMGFR
                     GGKGVATALGMFLGLAPLAVAIALPVWGGMLWLTGYVSLSSVIAAASFPVWVRLSQPA
                     ALPAFWASVGLAALIVFSHRANLRRLAAGTENRFRTRKGLP"
BASE COUNT          597 a         1357 c         1470 g          677 t
ORIGIN      
        1 cgctggcggg gatctatgcc caggccggtc gccgcgcaga ggcagcggcc gtgttcgacg
       61 acctcctcgc ccactcctcc gacctcacca cgtccgatct ctacgacctg ggccagcggc
      121 tgctacaggc gaaattgctg gggccgggga ccaaagccta cacgctcgcc ttgcagcaga
      181 atccgtatca tcgcaacgcc ctcgccgaac tcgcgagcgc ctacatcgac gccagggaca
      241 cggcgaacgc cctggccacg gcccagcgcc tccaggcgct ggacccactg aacaaggccg
      301 cgctccacat cgtcgcgcag gcgtgggagc tgcggggtcg gcgtgactcc gcccaggtgt
      361 acggcacgct ggccgacacc ctctccctcg acatctcgat cgccagcctg gtgggggaca
      421 gcgccggcgt caccgtgacc ggcgtcgggt ccaacctggg gaacggtccc tcgaaggcgc
      481 tgcgcatcac ggtggatcta ctggacgccc gcggcaccgc cgtcgcgtcc caggccgtgg
      541 aggtgccggc gctgcagccc ggtggcagtc accaattcca ggtgaaggga gccggccggg
      601 gcatcgtcgg ctggcgttac cgggccggct aggcgatatt accggttgct caagtttcgg
      661 ccggggttga gtttgcgtcg ctcggcctga gaaaccgctt taaccatcgg gcaaccgtct
      721 ttgctgcgcg tagccgcggt cgcgcctgag tccatcggcg ccgagttggg tctcctgccg
      781 gggaccgagc tgctcagcgt gaacgatcgg cggctggagg acttcctgga ttgggagttc
      841 ctcacggccg acgaggcgtt cgtgctgcgc gcccggttgc cgtccggcca ggagctggaa
      901 tgcgatatcg agcggcccga gggcatgccg ctcggggtgg cgctggagcc gccgcgcatc
      961 cgccgctgcg ccaatcactg cgacttctgc ttcgtggacg gcaatcccgc cggactgcgc
     1021 agcagtctct acatccgcgc cgacgattac cggctgtcgt tccgctacgg caacttcgcg
     1081 acgctcacca acctgaagcc ctgggacgag gcgcgcatca tcgagtaccg gctctcgccc
     1141 ctctacgtgt cggtccacgc gacggacccc gtggtgcgcc gctggttgct gcgcaacccc
     1201 gaggccccgg acatcgtgac ccagctgtcc gacttcgcgg cgcacggcat ccggtttcac
     1261 acgcaggtcg tgctcgtgcc cggcgtgaac gacggcgcgg cgctggagcg cacgctacag
     1321 gatctgtatg aactgggcgc ggcggtcctg tcggtctcga tcgtccccgt cgccctgacc
     1381 gagtacagca agcacagcct ggtccgcgcc atcagtccgg acgattgccg ggaggcgctg
     1441 gccaccgtgg accgctgggc gacacgggcc gtggcggaac gggggcaccc gtggtgcttc
     1501 ggggccgacg agctctatct cgtggccggt gcggcgctcc cgccggccgc ctggtacggg
     1561 gagttcgagc aacgcgaaaa cggcgtgggc gcggtgcgat acctgcagac gcgcatcgtc
     1621 gaggcgtcgg gcacctatgg cgactggacg gggaagcgga tcggcatcgc cacagggacc
     1681 gccatggcgc cgctgatgcc gcaggtgatc gagacgctga tgaaggagac cggcggccgc
     1741 ttcgaggttc tggcgctcga gaaccatctg ttcggggcgt cggtcaccac ggccggattg
     1801 cttcccgggg ccgcgttcct cacggcgctg cgcgaacggc gtgacctcga tctggctcta
     1861 ctgccggcgg aagccgtgaa cgacgacttg cgcttcatgg acgacatgga tgcccatgac
     1921 ctcgcggcgg cggtcccgat ggagattcgg ttctcgtacg actttgctga tgtgctgagc
     1981 gaacgcggaa tgcggaatga tgaatgcgga attgagccga gtgactccgt gcggcaggag
     2041 cctcgcccat cgaattccac attcgacatt ccgcattccg catggtggga ggggccgtga
     2101 agggcgctcc gaccgtcgcc atcatcggtc gcccgaatgt gggcaagtcg accttgttca
     2161 accggctgat cggccggcgc gaggcgatcg tggatgcgca gccgggggtg acacgcgacc
     2221 gcaacttcgc ggccgccgac tggaacgggc gccgtttctg gttggtggac acgggcgggc
     2281 tcgtccccgg tgcgtcggac gcgttgaacc gggcgattcg tgcccaggtc gaggctgcgg
     2341 tggcggagag tgacgtgctg ctcttcctgg tggatgtgga ggagggagtc catcccgccg
     2401 atctcgagat cgcgcagtac ctgcgcggcg tcgaccgtcc ggtgctgctg gtcgccaaca
     2461 aggccgaccg gctgcccgac gatcagcgcc acctggcgtt ctacgagctg ggcctggggg
     2521 atcccctccc cgtatcggct gccatcggca agagcagcgg ggatctgctc gaccgcatcg
     2581 tggagatgtt gcccacgcgg gaagggggcg acgacgagag cgccgtccag gtcgcgatcg
     2641 tggggcggcc caacgtcggc aaatcgagcc tcctgaaccg gctgctggga cgggatcgca
     2701 tggtggtggc ggacgagccc ggcaccaccc gcgatgccgt ggacaccccg gtggagcacg
     2761 agggccggcg cctcgtgttc atcgacaccg cgggcctgcg caagcggagc aaggtccagg
     2821 acgacgtcga gttctacgcc acgctgcgca cctcgcgggc catcgagcgg gccgacgtgt
     2881 gcgtgctcgt ggtcgatgcg ctggacggga tgcatgccca ggacctgaag atcatcgggg
     2941 acgcctggga gcgtggggca ggggtgatcg tggccgtgaa caagtgggat ctgatcgagg
     3001 agaaggagac gaacaccgcg gtccgcggtg agcgggagct cgtcgcgaag gcgccctttc
     3061 tgcggttcat tccgtttctc tacgtctcgg ccaagacggg ccagcgcgtg tcgaagttgt
     3121 tcgacctgat tctcacggtc gccgaggagc gtaccaagcg ggtgcccacg gcggaggtca
     3181 accgcgtgct cgagtcgctg gtgagtcgcc agcagccgcc gcagccggtg ggagaatcgg
     3241 tgcggctgtt ctacgcgtcc cagatcggta ccgccccgcc gcgcttcgcc atcgtcgcca
     3301 accgccctga tgccatcccc gagtcgtaca cccgctatct cgtgaacggt ttccgggagg
     3361 cctgggcgtt taccggggcg cccgtcaacg tcaagttccg ccgcaaacgc caggagcggc
     3421 ggtgacgatc gccctgtggc tcgcggccgc ctacctgctc ggcgcgacgc cgacggctta
     3481 cctggcggga cggctcgccc gggggatcga tctgcgcgag cacggcagcc gcaacctcgg
     3541 tgcgaccaac gtgtaccgcg tgctgggatg gcgctgggcg attccggtgg cactgttgga
     3601 catcgccaag ggcgccgtcc ccgtggtcct gttcgcccgc tgggccggga tgggtccatg
     3661 gggcgccgtg gccctgggga tcgccgcggt gctggggcac gtgttctcgc cgtatatggg
     3721 attccgggga gggaaagggg tcgccacggc gctcgggatg tttctgggcc tcgcgcccct
     3781 ggcggtggcg atcgcgttgc cggtgtgggg gggaatgctg tggctcaccg gatacgtgtc
     3841 gctcagctcc gtgatcgccg cggcgagctt cccggtatgg gtgcgactct cccagccggc
     3901 ggcactcccc gccttctggg cgagtgtggg cctcgccgcg ctcatcgtct tctcgcatcg
     3961 cgccaatctg cgccgcctcg cggcgggtac cgagaaccgc tttcgcaccc ggaaggggct
     4021 tccgtgagcg cgccggcgag cgaggtgcgg cgagtggccg tgatcggggc cggggcgtgg
     4081 ggcaccacgc tcgccgattt g
//
LOCUS       DBNGZW010000774         4457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_749846, whole genome shotgun sequence.
ACCESSION   DBNGZW010000774 DBNGZW010000000
VERSION     DBNGZW010000774.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_749846"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="AC16JC_18075"
     CDS             complement(<1..877)
                     /locus_tag="AC16JC_18075"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5166667.1"
                     /translation="MTAPQPVALVLVVDDEEPIRNALQRYLTKQGFDVMVAASGEEAL
                     GVLRQHDAIGLMVCDIMMPGMSGVDIVPQALEVSPDLAILMLTAVNDATTAALCMQRG
                     ALDYLTKPIELQELTRTLQRALKRRELLIGQRHLEQQIKEEVARRTAEFQRERARLER
                     VSVATLEVLVAALEAKDPYFLGHSARVADFSATLAAGMGLQDDEVEQVRLAGRLHDIG
                     KIGTREDIVNKRGRLTPEEHEHIQRHVIIGSQSLAPLDHLGPVIDIVLSHHERLDGTG
                     YPDRRKGEEIPLLGRV"
     gene            1292..1696
                     /locus_tag="AC16JC_18080"
     CDS             1292..1696
                     /locus_tag="AC16JC_18080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166668.1"
                     /translation="MSLPHEDQATTLTDAQATVGSPARAPRIRPKRGWPEGDHKVTIA
                     EARELVERRRRAFEQTGGAFKKSALERLLNQTGCVGMRIYYGMHPDGQPALILVGVDE
                     NGEELLDGELLERNFPCPPFCPLGPSLRRSRI"
     gene            1705..2364
                     /locus_tag="AC16JC_18085"
     CDS             1705..2364
                     /locus_tag="AC16JC_18085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166669.1"
                     /translation="MQYPMVLYAASVSQALPLVAGWMRWGRPLPAPARFVLVWCGVLL
                     ATDVLSIAVARAQGHNLWLQYAAVPIESGLILWALSGWQSHEVFRLAYRIAIPVLAAA
                     TAVALLIRPPEQMLDEVVAPFHALVLLGASLHTLLDRALRAEGAIGWQPWFWIGLGLS
                     LYYASSVAIGPFAQALLASNVAWVRQAYLARAWISIFAFVLITTGILCPLFRPASGGR
                     S"
     gene            2325..3083
                     /locus_tag="AC16JC_18090"
     CDS             2325..3083
                     /locus_tag="AC16JC_18090"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR5166670.1"
                     /translation="MSALPPGVWWAFLTAGLALAVILLALGAALVIYQRRFVALHRQH
                     AAGLLRAQEEERAWVARELHDDVLQRIAVVLYEVDRRATGGASARPAPLRTELEDLAM
                     VVRRVANHLHPAFLEREGLLPALQQLAAEVRDRSGVVLAVIEEPGNSPLNATTDQALV
                     AYRIAQEAIANLVRHSGATAGEVRLRAATDAFELEIRDRGRGIAPEQRWKAGHLGLIS
                     MEERARAAGGRLMVTSRSGEGTAVRLRLPCEGPA"
     gene            3101..3760
                     /locus_tag="AC16JC_18095"
     CDS             3101..3760
                     /locus_tag="AC16JC_18095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR5166671.1"
                     /translation="MVADDHPLMAQGLRALIAPFCDVVALVEDARYVVAAVEEHRPDL
                     LLLDLSMPHRNGLELLLALRKRFRDLRVLVVTMHLDRTLAEQAFLNGADGFIPKEAPA
                     DELHTAIATVLHGRRYLSPRVPRRGFRGDEALQDTLTERLTPRQREVLRLMGEGKTTP
                     QIAAALGLSQRTVEFHRAGARRALGITSEWELVRFAIMVRLGTPGDTPPLPAPETAGE
                     G"
     gene            3878..4120
                     /locus_tag="AC16JC_18100"
     CDS             3878..4120
                     /locus_tag="AC16JC_18100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166672.1"
                     /translation="MHPLVPPLRPAVAGALSLGGELLGRSLDLVPQVRRLRFASPMHD
                     VGKIGIPDGIPVKSGTLDPTEQRVRRHKQRHVVGLE"
     gene            4117..>4457
                     /locus_tag="AC16JC_18105"
     CDS             4117..>4457
                     /locus_tag="AC16JC_18105"
                     /inference="COORDINATES: protein motif:HMM:NF017077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HIR5166673.1"
                     /translation="MTAGREVAQRRALSLERKILLGFAASLVILVAVGAAALWSTSAT
                     AESATSVAQAFQVRAAVEGGLRGLLDAEASAREYLMTGQPTLLASHDSAVAHLGGLLE
                     RLRTLAADNPD"
BASE COUNT          657 a         1501 c         1557 g          742 t
ORIGIN      
        1 ggacgcgccc cagcagcggg atctcctctc ccttgcggcg gtcggggtag ccggtcccat
       61 ccaagcgctc gtgatggctc aatacgatgt cgatcaccgg gccgaggtgg tcgagcggcg
      121 ccaggctctg cgagccgatg atcacgtgcc gctggatgtg ctcgtgctcc tccggtgtta
      181 gtcggccgcg cttgttcacg atgtcctcgc gcgtgccgat cttgccgatg tcgtgcagcc
      241 gccccgcgag ccgcacctgc tcgacctcat cgtcctgtag gcccattccg gccgccaggg
      301 tggccgagaa atcagcgacg cgagccgaat ggccgaggaa gtacgggtct ttcgcttcga
      361 gcgccgcgac gaggacttcc agcgtggcga cggacacccg ttccagccgc gcccgctccc
      421 gctgaaactc cgccgtgcgc cgtgccactt cctctttgat ctgctgctcg aggtgtcgct
      481 ggccgatgag cagctcacgg cgcttcaacg cccgctgcag ggtccgggtc agttcctgga
      541 gctcgatcgg tttcgtcagg taatccagcg ccccccgctg catgcacaaa gccgcagtgg
      601 tcgcatcgtt gaccgcggtc agcatgagga tcgcgagatc gggcgatacc tcgagcgcct
      661 gcggcacgat gtcgactccg ctcatccccg gcatcatgat gtcgcacacc atgaggccga
      721 tcgcgtcgtg ttgtcgcagc acacccaggg cttcctcccc cgaggcggcg accatcacgt
      781 cgaacccttg cttggtcaag tagcgttgca gcgcattgcg gatcggttcc tcatcgtcca
      841 cgaccagcac gagggcgacg ggctgcggtg cagtcatgcg cgaactcccg cgtgcgctgg
      901 aaggggcatc gcctattcta cgaccgagcc gtaacgaacg tcaggggtcg cggcccccgg
      961 ccgggaggac gatggggatg cgcctatcag tatcggtacc tatcagggtc aggatcaata
     1021 caggctcagg atcagtaata gaacacggtc agcacatgtc gatattctac attctctcca
     1081 ggctcgggct gcgaaacaac gccgatctgg tgcgctatgc actggagcac tgcttagcgg
     1141 cagcatccgc gatctccggc ccaggcgcca cctagggcaa cccacagtgt tcaaaaccct
     1201 cctaccgaag gcacccttcc gcgcgacgcg cgagatcccg cgcgtccgga accgactggt
     1261 tccgcgacac tttacgcgtt tggagggtgc catgagcttg ccacacgaag accaggccac
     1321 cacgttgact gacgcccagg cgacggtcgg gtccccggcg cgggcgccgc ggatccggcc
     1381 gaagcgcggc tggcccgagg gtgatcacaa agtgacgatc gcggaggcgc gggagctggt
     1441 cgagcgccgc cggcgcgcct tcgagcagac gggcggggcg ttcaagaagt cggcgctgga
     1501 gcggctgctc aaccagacgg gctgtgtagg catgcggatc tactacggca tgcaccccga
     1561 cggccagcca gcgctgatac tggtgggtgt ggacgagaac ggcgaggagc tgctggacgg
     1621 cgagttgctc gagcggaact tcccgtgccc gccattttgc ccgctcgggc cgtcgttgcg
     1681 gcgttcgcga atctgaggag ggcgatgcag taccccatgg tgttgtacgc agcgtcggtg
     1741 tcgcaggcgc tgcccctcgt cgcggggtgg atgcgctggg gtcggcctct gccggccccg
     1801 gcgcggttcg tgcttgtgtg gtgcggagtg ttgctcgcga ccgatgtgct cagcatcgcg
     1861 gtcgcccgcg cgcaaggtca caatctgtgg ctgcagtacg cggcggtgcc gattgaaagc
     1921 gggctcatcc tgtgggccct gtcgggctgg cagtcgcacg aggtctttcg tctcgcgtac
     1981 cgcattgcca ttccggtgct cgcagcggcg accgccgtag ccctcttgat ccggcctccc
     2041 gagcagatgc tcgacgaggt agtcgcgccg tttcacgcgc tcgttctcct gggggcgtcg
     2101 ctgcacacgc tgctggaccg ggccctccgg gccgagggag cgatcggatg gcagccctgg
     2161 ttctggatcg ggctggggtt gtccctctat tacgcgagct ccgtggccat cgggccgttc
     2221 gcgcaggcac tgctcgcgtc caacgtcgcc tgggtgcggc aagcgtacct ggccagggct
     2281 tggatcagta tcttcgcttt cgtcctcatc accacgggca tcctatgtcc gctcttccgc
     2341 ccggcgtctg gtgggcgttc ctgaccgcgg gactggccct tgcggtgatc ctgctcgccc
     2401 tcggcgcggc gctggtgatc taccagcgcc gcttcgtggc tctgcaccga cagcacgcgg
     2461 cgggactgct gcgcgcccag gaagaagagc gggcgtgggt ggcgcgcgag ctgcacgatg
     2521 acgtgcttca acgcatcgcg gtagtgttgt acgaggtgga caggcgggcc accggcggcg
     2581 catcggcgcg tccggcgccg ctgcggaccg agttggagga cctagccatg gtcgtgcggc
     2641 gcgtggccaa tcacctgcat cccgccttcc tggagcggga gggcctgctc cccgccctgc
     2701 agcaactggc cgcggaggtg cgcgaccgct ccggtgtggt gctggcggtc atcgaggagc
     2761 ccggcaacag ccctctgaac gcgacgacgg accaggcgct cgtggcctac cgcatcgcgc
     2821 aggaagcgat cgccaacctg gttcggcatt cgggggccac cgccggagag gtgaggctcc
     2881 gggcagccac cgatgcgttc gagctggaga tcagggaccg tggccgcggc atcgccccgg
     2941 agcagcgctg gaaggcgggg catctcggcc tcatctcgat ggaggagcgc gcgcgggcgg
     3001 cggggggccg cctgatggtc acatcccggt ccggagaggg gacggcggtg cgcctgcgct
     3061 tgccctgcga gggtccggcg tgaacccgcg tcccctcgta atggtcgcag acgaccaccc
     3121 gctgatggcg cagggcctgc gagcgctgat cgcgccgttt tgcgacgtcg tcgcgctggt
     3181 cgaggacgct cgctacgtcg tagcggcggt ggaagagcac cgacccgacc tgctgctgct
     3241 cgacctctcc atgccacacc gcaacggcct cgaactgctg ctggccctgc gcaagcgctt
     3301 tcgcgacctg cgggtgctgg tggtgaccat gcacctggac cgcacgctcg cagagcaggc
     3361 gttcctgaac ggcgcggacg gtttcattcc caaggaagcg cccgccgacg agctgcacac
     3421 ggcgatcgcg acggtgctgc acggccggcg ctacctgtcg ccgcgtgtgc cgcggcgcgg
     3481 gttccggggc gatgaagccc tccaggacac gctcaccgag cggctcacgc cccgccagcg
     3541 cgaggtgctg cggctgatgg gagaaggcaa aacgacgccc caaatcgcag cggcgttggg
     3601 tctgagccag cgcaccgtgg agttccaccg ggcaggggcg cgccgggcgt tggggatcac
     3661 ctcggagtgg gagctggtac ggttcgccat catggtgcgg ctcggcacgc ccggcgacac
     3721 cccgccgctc cctgcgccgg aaacggcggg cgaaggatag ccttcgtgtc gaggcgcacg
     3781 ctcatttggt gagccggcag cgatcgcgcg ggtgatcccg agcccactag ggcaacccac
     3841 agcttgcagc ggtcccggtt ttccccacaa tacggtcgtg cacccccttg tcccgcccct
     3901 gcggccggcc gtcgcgggcg ctctgagcct gggcggcgag ctgctcggtc gcagcctgga
     3961 tctggtcccg caggtgaggc ggctccgctt cgccagcccc atgcacgacg tcgggaagat
     4021 cgggatcccc gacggcatcc cggtgaagtc cggcacgctc gacccgaccg aacagcgggt
     4081 gcggcggcac aagcaacggc acgtcgtggg actggaatga cggcggggag ggaagtagcg
     4141 cagcgcaggg cgctgtcgct cgagcgcaag atcctgctgg gattcgccgc ctcgctggtg
     4201 attctcgtcg cggtcggtgc ggcggccttg tggagcacgt ccgcgaccgc cgagagcgcc
     4261 acctccgttg cccaggcgtt ccaggtgcgt gcggccgtgg aaggcgggct gcgcgggctc
     4321 ctcgacgcgg aagcgagcgc gcgcgagtac ctcatgacgg ggcaaccgac cttgctcgcg
     4381 tcccacgatt ccgccgtcgc ccacctgggc gggctgctcg agcgcttgcg cacgcttgcg
     4441 gcggacaatc cggacga
//
LOCUS       DBNGZW010000775         1965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1769138, whole genome shotgun sequence.
ACCESSION   DBNGZW010000775 DBNGZW010000000
VERSION     DBNGZW010000775.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 1965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1965
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1769138"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            318..>1965
                     /locus_tag="AC16JC_18110"
     CDS             318..>1965
                     /locus_tag="AC16JC_18110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012097208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR5166674.1"
                     /translation="MTTTAVEPKPRVSADEARAVAEAAREEQWAAPSFVRDLFLGHLR
                     TDLIHPYPLQDPQEAERARPFLEQLTRLLSQVDSDRIDREGEIPPTVVQGLKDIGAFG
                     IKIPREYGGLGLSQLSYMKAIELVSSVDGSITALLSAHQSIGVPQPLKMFGTEEQKKE
                     YFPRLAKGAVSAFALTEPDVGSDPAALETTAVPSADGREWVLNGSKLWCTNGTIAELM
                     VVMARTPSKTVGGREKRQITAFIVEANAPGVKVDHRCHFMGLKAIYNGVVSFTNVRVP
                     AENILWGEGKGLKLALITLNTGRLTLPIAAVAAGKRCLEISRAWANERVQWGKKIGQH
                     DAVAQKIGTMAANTFAMEAVAELCGALADGGKSDIRLEAAIAKLYNSEAGWRIIDDTL
                     QVRGGRGYETADSLRARGEKAVPVERIMRDFRINLIFEGSSEIMHLFIAREAVDKHLQ
                     VAGDVVMPGKAAGQRLAGLVRSALFYGWWYPSRWFGWSFWPKYGEFGELAKHVRFVER
                     SARRLARGVFHAMMRFGPKLEQRQGVLFRLVDVGAELFAMA"
BASE COUNT          325 a          651 c          678 g          311 t
ORIGIN      
        1 gagcgcgaac cctgtgaggg cggtgaggag gcgtcgcact actgggaaag gtacacggtg
       61 ccatccttca taacgaacct gacccgttgg agttccgtga tgcccttgag cgggtcgccg
      121 gcatccacac cacgctgatc cgctggtctg cgatcagcat ccccacgttg gcctgtgcct
      181 gctcggatcg tccgtcgatc aggcggccgc ttttatgaag acgcgcgcgg caggccgcga
      241 atctccccgt gaagaccggg gtgtcaaggt gacgtgcgtc cccgattttt gggcgtcgcc
      301 ccgctacatt atttcgtatg accaccaccg ctgtcgaacc caagcccaga gtcagcgcag
      361 acgaggcgcg cgccgtcgcc gaggcggcgc gcgaggagca gtgggcggcg ccgagcttcg
      421 tccgggatct gttcctcggg catctgcgga cggacctgat ccatccgtat cccttgcagg
      481 acccgcagga agccgagcgg gcccggccgt tcctggagca gctcacccgg ctcctgtcgc
      541 aggtggattc ggaccgcatc gaccgcgaag gcgagatccc gcccaccgtg gtccagggcc
      601 tgaaggacat cggggcgttc ggcatcaaga tcccgcgcga atacggcggc ctggggctgt
      661 cgcagctctc ctacatgaag gcgatcgagc tcgtgtcgtc ggtggacggc tcgatcaccg
      721 cgctgctctc ggcccaccag tccatcggcg tgccgcagcc gctgaagatg ttcggtaccg
      781 aggagcagaa gaaggagtac ttcccgcggc tggccaaggg tgcggtgtcg gcgttcgcgc
      841 tcaccgagcc cgacgtcggc tccgatcccg cggcgctcga gacgaccgcc gtcccctcgg
      901 ccgacggccg cgagtgggtg ctcaacggct cgaagctgtg gtgcaccaac ggcacgatcg
      961 ccgagctgat ggtggtgatg gcgcgcaccc cgtcgaagac cgtcggcggc agggaaaagc
     1021 ggcagatcac ggcgttcatc gtcgaagcga atgccccggg cgtaaaggtg gaccatcgct
     1081 gccacttcat gggactgaag gcgatctaca acggcgtggt gtccttcacc aacgtccgcg
     1141 tccccgccga aaacatcctc tggggagagg gcaaaggcct gaagctcgcg ctcatcacct
     1201 tgaacaccgg ccgcctcacg ctgcccattg ccgccgtcgc cgccggcaag cgctgtctcg
     1261 agatctcgcg cgcctgggcg aacgagcgcg tgcaatgggg caagaagatc gggcagcacg
     1321 acgccgtggc acagaagatc ggcaccatgg cggcgaacac cttcgcaatg gaggccgtcg
     1381 ccgagctgtg cggcgcgctg gcggacggcg gcaagtccga catccggctc gaagcggcga
     1441 tcgccaagct gtacaactcc gaggccggct ggcgcatcat cgacgacacg ctgcaggtcc
     1501 ggggcggccg cgggtacgag accgccgaca gcctgcgcgc gcgcggcgag aaggcggtgc
     1561 cggtcgagcg catcatgcgc gacttccgca tcaacctgat cttcgagggg tcctcggaga
     1621 tcatgcacct gttcatcgcg cgcgaggcgg tggacaaaca tctccaggtg gcgggcgacg
     1681 tcgtgatgcc cggcaaggcc gccggccagc ggctcgcggg cctcgtccgc tcggcgctgt
     1741 tctacggctg gtggtacccg tcgcgctggt tcggctggag cttctggccc aagtacggcg
     1801 aattcggcga gctggccaag cacgtgcgct tcgtcgagcg cagcgcccgg cgcctggcac
     1861 gcggcgtgtt ccatgcgatg atgcgattcg gcccgaaact cgagcagcgg cagggcgtgc
     1921 tgttccgcct ggtggacgtg ggcgccgagc tgttcgccat ggcgg
//
LOCUS       DBNGZW010000776         2440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_474410, whole genome shotgun sequence.
ACCESSION   DBNGZW010000776 DBNGZW010000000
VERSION     DBNGZW010000776.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 2440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2440
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_474410"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..154
                     /locus_tag="AC16JC_18115"
     CDS             35..154
                     /locus_tag="AC16JC_18115"
                     /inference="COORDINATES: protein motif:HMM:NF015736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HIR5166675.1"
                     /translation="MVGKQRNGPTGKVDLYFKKEYTRFDNRSHRDAPPGAIGA"
     gene            126..1505
                     /gene="radA"
                     /locus_tag="AC16JC_18120"
     CDS             126..1505
                     /gene="radA"
                     /locus_tag="AC16JC_18120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015921988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR5166676.1"
                     /translation="MPRRGPSVPKPVSLFRCTDCGAEQPKWAGRCDACGAWNTLVEEP
                     LADRRSGGRAVGSARTFDRPPVRLSALSVQELARWQTGLGELDFVLGGGIVPSSVILV
                     GGEPGIGKSTLLLQAAAHLERAGVRTLYVSGEESPEQLRLRADRLDADAGAVHVLGET
                     NLEAILHHAGAIGAQAVFLDSIQTTYTEALEGAPGNVGQVRECAARLMRFAKEAGPAV
                     FLVGHVTKGGGIAGPRTLEHIVDTVLYFEGEATLDHRVLRAVKNRFGSVDEIGVFHMT
                     ATGLEPVADPAAAFLAGRHAGVSGSAVTALMEGTRPLLVEVQALASKPGFGTPQRVAT
                     GLDQRRLAVLLAVLERRGGLPVGALDVFVNVTGGVRLLEPSTDLAVLAALTSSVHDRP
                     VPADALFLGEVGLGGEVRPVAGLERRLTEAQRQGFRRVFLSSRSRVAGGLEVVAIAHV
                     GELARRLAA"
     gene            1471..2184
                     /gene="ispD"
                     /locus_tag="AC16JC_18125"
     CDS             1471..2184
                     /gene="ispD"
                     /locus_tag="AC16JC_18125"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015625903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HIR5166677.1"
                     /translation="MSVSSPAGSPPDVAVVIVAAGSGVRAGPGGPKQFRPIAGVPMLL
                     RALRPFLARPEVGRVVVTLPAGVAEQPPVWLKELLGPRVTGLSGGATRADSVRAGLRG
                     LGDLLPVVLVHDAARPFVDAATIRAVIEHARTGVGAVAAVPMGDTVKDVADDGRVVRT
                     VARIRLWRAQTPQGFPAAMLAAGYARWPADAPPPTDDAEAVERAGFPVEVVAGSAFNL
                     KVTTEDDWRLAEAMARELG"
     gene            2181..>2440
                     /locus_tag="AC16JC_18130"
     CDS             2181..>2440
                     /locus_tag="AC16JC_18130"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR5166678.1"
                     /translation="MTALPFVTIADAQGAVPRVKTHLRGGGLLAYPTETVYGLGSRPV
                     EPDLRALAALKGRAAGKPFLLLVASREMAEAQGLAMNESARAL"
BASE COUNT          305 a          818 c          955 g          362 t
ORIGIN      
        1 ggacggcaac agcatcgagg gactggccgc gctcatcgtc ggtaagcagc gcaacggtcc
       61 tacgggaaag gtggatctgt acttcaagaa ggaatacacg cgcttcgaca accggtcgca
      121 ccgcgatgcc ccgccggggg ccatcggtgc ctaagcccgt ttcgttgttc cgctgcaccg
      181 actgcggggc cgaacagccc aagtgggcgg ggcgctgtga cgcgtgcggg gcgtggaata
      241 cgctggtgga ggagccgctg gcggacagac ggtcaggcgg tcgggcggtc ggcagtgcac
      301 ggacctttga ccgaccgcct gtccgcctgt ccgccctgtc tgtgcaggag ctcgcccgct
      361 ggcagacggg gttgggagag ctcgatttcg ttctcggcgg cggcatcgtc ccctcgtcgg
      421 tgatcctggt agggggcgag ccggggatcg gcaaatccac gctgctgctg caggcggccg
      481 cccacctcga gcgtgcgggc gtgcgcacgc tctacgtgtc gggcgaagag tcgcccgagc
      541 agctgcggct gcgcgccgac cggctcgacg ccgacgccgg cgccgtccac gtgctgggcg
      601 agaccaacct cgaagcgatc ctgcaccacg ccggcgccat cggcgcccag gcggtcttcc
      661 tcgattcgat tcagacgacc tacacggaag cgctcgaggg cgccccggga aacgtgggtc
      721 aggtgcgcga gtgcgccgcc cggttgatgc gcttcgcgaa ggaagccggt ccggccgtgt
      781 tcctcgtcgg tcacgtgacc aagggaggcg gcatcgctgg cccgcgcacc ctggagcaca
      841 tcgtggacac cgtgctctac ttcgaggggg aggcgacgct cgaccaccgc gtcctccggg
      901 cggtgaagaa ccggttcggg tcggtggacg agatcggcgt gttccacatg accgcgacgg
      961 ggctcgagcc ggtggccgat cccgcggccg cgttcctcgc ggggcgccac gccggggtgt
     1021 ccggttcggc cgtgacggcg ctgatggaag gcacacgccc gttgctggtg gaggtgcagg
     1081 cgctcgcgtc caagccgggc ttcgggacgc cgcagcgggt ggccacggga ctcgaccagc
     1141 ggcggctcgc ggtgctgctc gcggtactgg agcggcgcgg ggggctcccg gtcggggccc
     1201 tcgacgtgtt cgtcaacgtc accggcgggg tccgcctgct cgagccgtcc accgatctcg
     1261 cggtgctcgc ggcgctgacc tcgagcgtgc acgaccggcc cgtaccggcc gatgcgctgt
     1321 tcctgggcga agtgggactg ggtggggaag tgcgccccgt cgcggggctc gagcgccgcc
     1381 tgaccgaagc ccagcgccag ggattccgtc gcgtctttct ctcgtcgcgg tcccgcgtgg
     1441 ccggcggact cgaggtcgtg gcgatcgcgc atgtcggtga gctcgcccgc cggctcgccg
     1501 cctgacgtcg ccgtcgtgat cgtggcggcc gggtccggcg tgcgcgccgg tcccggcggc
     1561 ccgaagcagt tccggcccat cgccggcgtg ccgatgctgt tgcgggcgct gcggccgttc
     1621 ctggcgcggc ccgaggtagg gcgcgtcgtg gtgacgttgc ctgccggcgt cgccgagcag
     1681 ccgcccgtgt ggctcaagga gctgctgggg ccacgggtga ccggtctgag cggcggggcc
     1741 acgcgcgccg attccgtgcg ggccgggctc cggggactgg gcgacctcct gcccgtggtg
     1801 ctggtccacg atgccgcgcg gccgtttgtg gacgcggcca cgatcagggc cgtcatcgag
     1861 catgcgcgca cgggcgtcgg cgcggtggcg gcggtaccga tgggcgacac ggtgaaggac
     1921 gtggcggacg acgggcgggt ggtgcggacg gtggcgcgga tccgcctgtg gcgggcccag
     1981 acgcctcagg ggttccccgc tgcgatgctc gccgcagggt acgcacgctg gccggccgac
     2041 gcccccccac ccaccgacga tgccgaggcg gtcgagcggg cgggtttccc ggtggaggtc
     2101 gtagccgggt ccgccttcaa cctgaaggtg acgaccgagg acgactggcg cctggcggag
     2161 gccatggcgc gggaactggg atgacggccc tgccgttcgt gacgatcgcg gacgcccagg
     2221 gggccgttcc gcgggtaaag acccacctgc gcgggggggg cctgctcgcg tatcctacgg
     2281 agacggtgta cggtttggga tcgcggcccg tggagcccga cctccgtgca ctggcggcgc
     2341 tcaagggccg ggcggccggg aagccgttcc tgttgctggt ggccagccgc gaaatggccg
     2401 aggcgcaggg gctcgccatg aacgaatccg cccgcgcgct
//
LOCUS       DBNGZW010000777         4153 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1088369, whole genome shotgun sequence.
ACCESSION   DBNGZW010000777 DBNGZW010000000
VERSION     DBNGZW010000777.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4153)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1088369"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /locus_tag="AC16JC_18135"
     CDS             <1..797
                     /locus_tag="AC16JC_18135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018232135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR5166679.1"
                     /translation="QRIVRAKRTLTDAHVAFEVPRGPELAGRLASVLEVIYLVFNEGY
                     SATAGDDWARPELCNDALRLARILAELAPQEPEVHGLVALLEIQASRLHARIGPSGEP
                     ILLLDQDRARWDQLLVRRGVAALERAEALGGSLGPYALQAAIAACHARARMPEETDWT
                     RITALYDALAQLTPSPIVELNRAVAYAMAFGPAAGLEIADPLTSEPALDGYHLLPAVR
                     GDLLAKLGRLAEARAEFERAAALTRNTRERTLLLERARGCERPEIA"
     gene            839..1294
                     /locus_tag="AC16JC_18140"
     CDS             839..1294
                     /locus_tag="AC16JC_18140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR5166680.1"
                     /translation="MAARWAAAGPQLQSLLRIFAAFIFVQSGTLKLFAFPIGMPPNGS
                     TAVFLTEIWFAGVLETFGGALLLLGLFTRPVAFLLAGEMAVAYFQFSSPLSFWPVANN
                     GVAAALYCFVWLYYSAAGAGPWSLDARRGTVTAAAGPAGPRAETGPARR"
     gene            complement(1236..2414)
                     /locus_tag="AC16JC_18145"
     CDS             complement(1236..2414)
                     /locus_tag="AC16JC_18145"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007418459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR5166681.1"
                     /translation="MTHYPYLIVGGGMTGDAAARTIREVDPAALVGLISAEEHPPYAR
                     PPLSKGLWKGDPESGIWRGTEASGVQLRLGRRVTAIDAAARTVTDDRGEAVSYGRLLL
                     ATGGAPRRLPRDTEQIIYFRTYDDYRRLHTLAGESLRFVVLGGGFIGSEIAAALRTAG
                     REVVMLVPEDGIGARVFPTDLSASLVDYYREQGVEVRTGEGVAGLTPRAGKVILETTR
                     GRELSGDVIIAGLGIRPEVALAEQAGVHVENGIVVDELLRSSVPDIYAAGDVANFFNP
                     ALSKRLRVEHEDNANTMGAAAGRAMAGQGVPYTHLPFFYSDLFALGYEAVGELDARHE
                     TVADWKEPFREGVVYYLEGGRVRGVLLWNTWGQVDAARALIAEPGPFRPEDLRGRLPR
                     "
     gene            complement(2414..3049)
                     /gene="folE"
                     /locus_tag="AC16JC_18150"
     CDS             complement(2414..3049)
                     /gene="folE"
                     /locus_tag="AC16JC_18150"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR5166682.1"
                     /translation="MASLPLTELGGDTATEGRLRLAARRFRAFMEALDLDLADPHLAG
                     TELRVARAYHEMFAGLAPEAEPELRTFPNTERYAEMVRVTDIPFYSLCAHHFLPFFGT
                     AHVAYLPRERIVGLSKLARAVEYYARRPQVQERMTEQIVDLLDRRLRPAGVMVVVQAR
                     HFCIEMRGIGRSGLSTTTSAVRGAFDEPRRRREFLALLPRSGGSAPLHGSD"
     gene            complement(3059..3694)
                     /locus_tag="AC16JC_18155"
     CDS             complement(3059..3694)
                     /locus_tag="AC16JC_18155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014651658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR5166683.1"
                     /translation="MHPLLLVPALVAAPSHTARPPVLPAPMTAAAPVSRAAFHDAMRR
                     LWEDHITWTRLYIVSAAAGLPDADQTAQRLLRNQADIGDAVKPFYGDAAGAGLTALLR
                     GHILTAARLIGAAKGGDTAAVTAASAQWYANADSIATFLSSANPRNWPAETMRSAMRM
                     HLDLTLKEAVSRVHGDWAADIAAYDEVHRHILQMADVLSDGIVKQFPSRFN"
     gene            3817..>4153
                     /locus_tag="AC16JC_18160"
     CDS             3817..>4153
                     /locus_tag="AC16JC_18160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020672473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR5166684.1"
                     /translation="MPARKAEDTIARLAALTDPVRRALYFYVAHDPGEVSRDQAARGV
                     RIARPLAAFHLDKLVEQGLLQASFRRLSTRRGPGAGRPAKLYRRSALQVDLSLPAREY
                     ELAAKLFAGA"
BASE COUNT          603 a         1493 c         1429 g          628 t
ORIGIN      
        1 agcagcggat cgtgcgcgcc aagcggacgc tcaccgacgc gcacgtcgcg ttcgaggtcc
       61 cgcgcggccc cgagctcgcc ggccgcctgg cgtcggtgct cgaggtcatc tacctggtgt
      121 tcaacgaggg ctactcggcg accgccggcg acgactgggc gcggccggaa ttgtgcaacg
      181 atgcactccg gctcgcccgg atcctggccg agctcgcacc acaggagcca gaagtgcacg
      241 gcctggtcgc gctgctcgag atccaggcat cgcgcctgca cgcccggatc gggccctccg
      301 gcgagcccat cctgctgctc gatcaggacc gcgcgcgctg ggaccagctg ctcgtgcgcc
      361 gtggcgtcgc cgcgctcgag cgcgccgaag cgctgggcgg ctctctcggt ccctatgcgc
      421 tccaggcggc gatcgccgcc tgccatgcgc gtgcgcgcat gccggaggag acagactgga
      481 cgcgcatcac ggcgctgtac gacgccctgg cccaactcac accctcaccc attgtggagc
      541 tgaaccgcgc ggtcgcctac gccatggcct tcggccccgc ggccgggctc gaaatcgccg
      601 accccctgac ctccgagccc gcgctcgacg gctatcacct cctgcccgcc gtgcgcggcg
      661 accttctcgc gaagctcggc cgcctggccg aggcacgggc cgagttcgag cgcgcggccg
      721 cgctcacccg caacacgcgc gagcgcacgc tgctgctcga gcgcgcccgc gggtgcgagc
      781 gaccggaaat cgcgtgatgt aggagacgcc gaccatgcaa ccgacggttc tgcgaacttt
      841 ggcagcgcga tgggccgcag cgggcccgca gctccagagc ctgctccgca tcttcgccgc
      901 gttcattttc gtccagtccg gcacgctgaa gctgttcgcc tttccgatag gcatgccgcc
      961 gaacgggagc accgcggtgt tcctgaccga gatctggttc gccggagtcc tcgagacttt
     1021 cggcggggct ctgctgctgc tcggcctgtt cacccgcccg gtcgcattcc tgctggcggg
     1081 tgagatggcc gtggcctact tccagttctc ttccccgctg agcttctggc ccgtggcgaa
     1141 caacggggtg gccgcggcgc tatactgttt cgtctggctt tactactcgg cggctggtgc
     1201 cggtccctgg agcctggacg cgagacgcgg cacggtcacc gcggcagccg gccccgcagg
     1261 tcctcgggcc gaaacgggcc cggctcggcg atgagcgcac gcgcggcgtc cacctggccc
     1321 cacgtgttcc acagcagcac gccgcgcact cgtcccccct cgagatagta gaccacgccc
     1381 tcgcgaaacg gctccttcca gtccgcgacg gtctcgtgcc gggcgtccag ctcacccacg
     1441 gcttcgtacc cgagcgcgaa caggtcggaa tagaagaagg gcaaatgcgt gtaggggaca
     1501 ccctgccccg ccatcgcgcg ccccgccgcg gcgcccatgg tgttggcgtt gtcctcgtgc
     1561 tcgacgcgca gtcgcttgct caacgcgggg ttgaagaagt tggcgacgtc gcccgccgca
     1621 tagatgtccg gcacgctcga gcgcagcagc tcgtccacca cgatgccgtt ctccacgtgc
     1681 acccccgcct gctcggcgag ggcgacctcg ggccggatac cgagcccggc gatgatcacg
     1741 tccccggaga gctcgcggcc gcgcgtggtc tcgaggatca ccttgccggc gcgcggggtg
     1801 agccccgcca cgccttcgcc cgttcgcact tccacgccct gctcgcgata gtaatcgacc
     1861 agcgacgcgg acagatcggt cggaaacacg cgggcgccga tcccgtcttc gggaacgagc
     1921 atcacgacct cccgtcccgc cgtgcgcagc gcggccgcga tctcgctgcc gatgaagccg
     1981 ccgcccagca ccacgaaccg caggctctcg cccgccaggg tatgcaggcg acggtagtcg
     2041 tcgtaggtgc ggaagtagat gatctgctcc gtgtcgcgcg gcagccggcg cggtgcgccg
     2101 ccggtcgcca gcagcagccg accgtaggag acggcctcgc cgcgatcgtc ggtgacggtc
     2161 cgggcggcgg catcgatcgc cgtgacccga cgtccgagcc gcagctgcac gccggatgct
     2221 tcggtgccgc gccagatgcc ggactccggg tcgcccttcc acaggccctt ggaaaggggt
     2281 gggcgggcgt agggaggatg ctcctcggcc gagatgaggc cgaccagcgc ggccggatcg
     2341 acctcgcgaa tggtacgggc ggcggcgtcc cccgtcatgc cgccgccgac gatcagatac
     2401 ggatagtgcg tcatcaatcg ctcccgtgca gcggggccga gccccccgac cgcggcagta
     2461 gcgccagaaa ctcccggcgt ctacgcggct cgtcgaacgc cccccgcacc gcgctcgtcg
     2521 tggtcgagag tccggagcgt ccgatccccc gcatttcgat gcagaagtgc cgcgcctgga
     2581 cgacgaccat cacgcccgcc ggccgcaggc ggcggtccag cagatcgacg atctgctcgg
     2641 tcatgcgctc ctgcacctgc ggacgtctcg cataatactc gactgctcgc gccagcttgg
     2701 acagtcccac gatccgctcc cggggcagat acgccacgtg cgcggtcccg aagaacggca
     2761 ggaaatggtg agcgcacagg gagtagaacg ggatgtccgt cacccgcacc atctccgcgt
     2821 agcgctccgt gttgggaaac gtccgcagct ccggctcggc ttcgggtgcc aggcccgcga
     2881 acatctcgtg gtaggcccgg gcgacccgca gttccgtgcc ggccaggtgc gggtcggcca
     2941 ggtcgagatc cagggcttcc atgaaggcgc ggaacctccg cgccgcgagc cgcagcctgc
     3001 cctcggtagc ggtatcgccg ccgagctcgg tgagagggag actggccatg ggcgtacctc
     3061 agttaaaccg gctggggaac tgtttgacga tcccgtcgct caagacatcg gccatctgga
     3121 gaatgtgccg gtgcacctcg tcgtacgcgg cgatgtcggc ggcccaatcg ccatgcaccc
     3181 ggctgaccgc ctccttgagg gtcaggtcga ggtgcatgcg catggccgac cgcatggttt
     3241 cggccggcca gttgcgcggg ttggccgagc tgagaaacgt cgcgatgctg tcggcgttgg
     3301 cgtaccactg ggccgaggcc gcggtgacgg ccgcggtgtc gccgcccttg gcggcaccga
     3361 tcagcctggc cgccgtgagg atatgcccgc gcagcagcgc cgtcagaccc gcgccggccg
     3421 cgtccccgta gaacggcttg acggcgtctc cgatatcggc ctggttgcgc agcaaccgct
     3481 gcgcggtctg gtcggcgtcg gggaggccgg cggcggcgct gacgatgtag agacgcgtcc
     3541 aggtgatgtg atcctcccac agccggcgca tggcgtcgtg aaacgcggcg cggctgacgg
     3601 gggcggcggc ggtcatgggg gccggcagga ccggcgggcg cgcggtgtgg gacggagcgg
     3661 cgaccagcgc cggaacgagc aggagcggat gcatggcgtc cacctcttca tgaagggaat
     3721 gggaccgttg cttctaaaac aagtgtacgc taattgttga gtgccggatc tgtgctgggg
     3781 ggtttcgcta gtggccgcaa gcgatagatt gcaggcatgc cggcgcggaa ggcggaagac
     3841 acgattgcgc gactcgccgc gctcaccgac ccggtgcggc gcgccctcta cttctatgtc
     3901 gcccacgatc cgggcgaggt gagccgggat caggcggccc ggggcgtgcg catcgcgcgg
     3961 ccgctcgccg ccttccacct ggacaagctg gtcgagcagg gactgctcca ggcgagcttc
     4021 cgccgcctgt ccacgcgccg cggaccgggc gccgggcgcc ccgccaagct gtaccgccgc
     4081 tcggccctcc aggtcgacct ttcgctcccg gctcgcgagt acgagctcgc cgcgaagctc
     4141 ttcgccggcg cgc
//
LOCUS       DBNGZW010000778         8789 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1839831, whole genome shotgun sequence.
ACCESSION   DBNGZW010000778 DBNGZW010000000
VERSION     DBNGZW010000778.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 8789)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8789)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8789
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1839831"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /locus_tag="AC16JC_18165"
     CDS             <1..885
                     /locus_tag="AC16JC_18165"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR5166685.1"
                     /translation="QKLTRARVQLVFNMAEGLYGQNREAHVPAICEYLNVPYTASDPL
                     TLSLNKARAKEILSYHDVPTARFAVVADLGDLTRVRLPYPLFVKPVAEGSGKGIFANN
                     LCHHRAQLRERVSFLLETYKQPVLVETYLPGPEFTVAIIGNGADAFCLPIVGFDFSTL
                     PPGATPVYGFEAKWIWDTAEHQLDIFECPARIPEKLYRHIEIVALDAYQALGCRDWCR
                     IDVRVDEDGVPNVVELNPLPGILPDPRMNSCFPKAARAAGFSYDELIQEVVRIAWKRV
                     TGREIMVPAAGLRAGATA"
     gene            882..1901
                     /locus_tag="AC16JC_18170"
     CDS             882..1901
                     /locus_tag="AC16JC_18170"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF019121.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HIR5166686.1"
                     /translation="MRVSILYDPGAEDWTAEDIAGVMKAVNEIARIFEAMGHTARKLP
                     VRHDMRWFSEARRSDLVFNLCEGVHGFSQWEEHVVAALEFAGVPVTGCGPWTIAACRR
                     KATANAILAQAGVPIPPWTIAQGKIDDAFPLPAIVKPAAEDASAGLDRGSVVTDRKAL
                     RAKIAAMTEQFDEVLVQQYIPGREFNVGFVGSRVLPVAEIDFTGMPEGSWPILTYAAK
                     WHTGSAEDLGSVPVCPAPVSQRLADRLVRVADLAWRTMQGKGYGRVDLRVDDTGRPWV
                     LEVNPNPDLNDDAGLSRMASAAGWDYPELIRRIAEGALREAQGAKAARELLAPSRRTR
                     APRTP"
     gene            1909..2355
                     /locus_tag="AC16JC_18175"
     CDS             1909..2355
                     /locus_tag="AC16JC_18175"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR5166687.1"
                     /translation="MRDTGLFREAEVATAVELLDEALDGDDDYRFLGAFDGSELVGYA
                     CYGPTPDTIGTWDLYWIAVDRTRQGTGIGTQLVTAVEHRLTAEGGRLIVVETSSRADY
                     TATRGFYEARGYGRTATIPGYYAPGDDLVIYTKDLTHGHDLAGAAP"
     gene            2324..3436
                     /locus_tag="AC16JC_18180"
     CDS             2324..3436
                     /locus_tag="AC16JC_18180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HIR5166688.1"
                     /translation="MDMTWQELLRKRSIASLEALAERFGAEHVGDIERLKQAAENFEF
                     RISPAMVDLIREPGDPIWRQYVPTVQELEIEDGIVDSLAEDAHSPVPNITHRYPDRAL
                     FLVSPVCAAYCRFCTRRRKVGDPEKIPMAHLESAFQYLAQHTEIRDVIMSGGDPLMLS
                     DRRLDEICGRLRAIPHLEILRIGSRVPCHLPERITPELCATLKKHHPLYINTHFNHPD
                     ELTPAAVKGLGMLADAGIPLGCQTVLLKGINDDPAVMKRLMQKLLAARVRPYYIYQAD
                     QVAGAEHFRTTVEKGLEIVQALRGWTSGLAVPHYVIDAPGGGGKIPLLPEYVLAIDED
                     QVVLRNYAGKTFVYRQPHEAGATPLIPLEFEPVLAG"
     gene            3524..3922
                     /locus_tag="AC16JC_18185"
     CDS             3524..3922
                     /locus_tag="AC16JC_18185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR5166689.1"
                     /translation="MRLITTVVRPERLPEVKAALFRAGVTGITLSRVTGHGGEREVIE
                     QYRGSTVVAEFHEKVKIEMAVSEPFVEPTIQAILSSARTGEVGDGKIFVQPLERVIRI
                     RTGEADNAALTAVNADEVQRAAIEAQAGER"
     gene            3919..5250
                     /locus_tag="AC16JC_18190"
     CDS             3919..5250
                     /locus_tag="AC16JC_18190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR5166690.1"
                     /translation="MSQVAQISSGDTAWVLTSSALVLFMTVGLALFYGGLVRSKNALN
                     TMMMSVVALGVIGVQWVLFGYSLAFAPGNAWLGGLAWLGFSGVGLDPNPTYAATIPQL
                     AHAVFQAMFAVITPALISGAIVERMRFRAYVAFLALWATLVYVPLAHWVWGAGGWLQQ
                     LGAIDFAGGTVVHISAGVSALVAARLLGPRQGFRRTPLVPHNVPLVLLGAGILWFGWF
                     GFNAGSALAANGIAVLAFVNTNSAAAAALVTWAALDGLRGGKVTAVGAATGAVVGLVG
                     ITPAAGYVTPRAALAIGALCAVVSYFAMQRRSRSGLDDSLDVFSCHGLAGIGGALLTG
                     VFASRAVNPGGADGVLAGHSAQLGIQVIAVLAAAGLAALGTIVIFWVVRAVFGVRAAV
                     RDEIAGLDLAEHGEEAYLGADLGGLTGPGVSLSEGVVLQGPPPGSPPQRAA"
     gene            complement(5284..6234)
                     /locus_tag="AC16JC_18195"
     CDS             complement(5284..6234)
                     /locus_tag="AC16JC_18195"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR5166691.1"
                     /translation="MMPRLARFLCCGLVLAACKPIDKGSTASQDSATPAPPPAPTATR
                     VSAVPGFSNPESVLHDSAQDVYFVSNINGSPTAKDGNGFISRVTPEGAVVSLRFIEGG
                     KNGVVLNAPKGMTLAGDTLWVTDIDAVRAFDARTGAPLDSVSLSTLTAVFLNDITATP
                     SGAIYITDTGIRFDDVGNVLHRGPDRVFRIGPDRTATVALRGDSLGWPNGIALDPVGR
                     RFIIGQFGGKALLAWKPEDRAPTVVATGPGGFDGIAMAGNRIFASSWNDSTVSSYETG
                     KEVKLITGVYGSADIGYDAKRNRVLIPVITGNRVEIWQLP"
     gene            complement(6255..7178)
                     /locus_tag="AC16JC_18200"
     CDS             complement(6255..7178)
                     /locus_tag="AC16JC_18200"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020039.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HIR5166692.1"
                     /translation="MRTLGIAALCVAAVAACTKEQPKPPQPPPPPTASKVAQIDSLQT
                     PESVLWDSAQDVYFVSSINGNPNVKDNNGFISRVKPDSGVENLRFIAGGQSGVTLNAP
                     KGLALHGDTLWVTDIDAVRAFNAKTGRSLGMVDLRAQGAVFLNDVVFGPDGALYITDT
                     GIQFDSAGTMSHPGTDRIYRVTSNRRVSVAAEGDSLRRPNGITWDATGNRFIVVAFGG
                     PTVFAWKLGDKMPTVIAQGPGSFDGVEVARGQLLVSSWADSTISAYQSGNAVKLISGV
                     PGPADFGYDARRNRVMIPLFTLNRVEIWQLP"
     gene            complement(7244..>8789)
                     /locus_tag="AC16JC_18205"
     CDS             complement(7244..>8789)
                     /locus_tag="AC16JC_18205"
                     /inference="COORDINATES: protein motif:HMM:NF047558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR5166693.1"
                     /translation="EMLTGRTPFTGPTAQAIIAQLFTEPPPPLRSVRPAEGTPEWAEQ
                     AVMKALAKDPAARFTTAAQFSQALTASGVTTPSGATPTAPAASAVKSIAVLPFVNMSA
                     DPENEYFTDGIAEEIINALTKVQALRVAARTSAFAFKGKNLDIRKVGEQLSVATVLEG
                     SVRKAGTRLRVTAQLVNVADGYHLWSERYDRELQDVFAIQDEIAGNIVTALRVVLSDQ
                     EKRAIEKAPTDNVQAYDCYLRGRQFFHQFRRTSIAFARRMFERAIEIDPGYALAYAGV
                     ADCCSFLYMYWDGSKANLEGAEAASRKALELDPELAEAHGSRGFAVSLSRRYDEARKE
                     FETAIRLNPKQFEAHYLYARACFQEGKLEEAVRHYEDAARVRPEDYQVPILMAAPLKG
                     LGRQQDMVAALRRGLEVAQRHLELNPDDVRALYLGAGALMQLDERERGLEWNRRAQAM
                     DSEDPAVLYNVACGFANAGEIEEALACIEKAIQNGFGHKEWLENDSDLDPLRAEPRFQ
                     AMLQRL"
BASE COUNT         1302 a         3105 c         2969 g         1413 t
ORIGIN      
        1 caaaaactca cccgcgcccg cgtccagctc gtgttcaaca tggccgaggg cctctacggt
       61 cagaatcgcg aagcgcacgt ccccgcgatc tgcgagtacc tgaacgtccc gtacacggcc
      121 agcgaccccc tcacgctgtc gctgaacaag gcgcgcgcga aggagatcct ctcctaccac
      181 gacgtcccga cggcccgctt cgccgtggtg gcggacctgg gcgacctgac gcgggtgcgg
      241 ctgccctacc cgctgttcgt caaacccgtc gcggaggggt caggtaaagg catatttgcc
      301 aataacttgt gccatcaccg cgcgcagctg cgcgagcgcg tctctttcct cttagagacc
      361 tacaaacagc cggtgctggt cgagacctat ctgcccgggc ccgagttcac cgtggccatc
      421 atcggcaacg gcgccgacgc cttctgcctg ccgatcgtcg ggttcgactt ctcgaccctc
      481 ccccccggcg cgacgccggt gtacggcttc gaggcgaagt ggatctggga caccgccgag
      541 caccagctcg acatcttcga gtgcccggcc cgcatccccg agaagctcta ccgccacatc
      601 gagatcgtcg ccctcgacgc ctatcaggcg ctcgggtgcc gcgactggtg ccgcatcgac
      661 gtccgcgtgg acgaagacgg ggtccccaac gtcgtcgagc tcaatcccct gcccgggatc
      721 cttcccgacc cgcgcatgaa cagctgcttc cccaaggccg cgcgggcggc cgggttctcc
      781 tacgacgagc tgatccagga ggtcgtccgg atcgcctgga agcgggtgac cgggcgggag
      841 atcatggtgc cggcagccgg gctgcgggcg ggggccacgg cgtgagggta tccatcctct
      901 acgaccccgg tgccgaagac tggaccgccg aggatatcgc cggagtgatg aaggccgtca
      961 acgaaatcgc ccgcatcttc gaagcgatgg ggcatacggc ccggaaactc cccgtgcggc
     1021 acgacatgcg gtggttctcc gaggcccggc gctcggatct ggtgttcaac ctgtgcgagg
     1081 gggtgcacgg gttctcgcag tgggaggagc acgtggtggc cgcgctcgag ttcgccggcg
     1141 tcccggtgac cggctgcggc ccctggacga tcgccgcctg ccgccgcaag gcgacggcca
     1201 acgcgatcct cgctcaggcc ggcgtgccga tcccgccctg gaccatcgcg cagggcaaga
     1261 tcgacgacgc cttcccgctc cccgcgatcg tcaagcccgc cgccgaagac gcgagcgccg
     1321 gcctcgaccg cggctcggtg gtcaccgacc gcaaggcgct gcgcgccaag atcgcggcga
     1381 tgaccgagca gttcgacgag gtgctggtgc agcagtacat ccccgggcgc gagttcaacg
     1441 tcggattcgt cggcagccgc gtgctccccg tcgccgagat cgatttcacc ggcatgcccg
     1501 aggggagctg gccgatcctc acctacgccg ccaagtggca cacggggtcg gcggaggacc
     1561 tgggcagcgt gcccgtgtgt ccggcgcccg tgtcccagcg gctcgccgac cgtctggtcc
     1621 gcgtggccga cctcgcctgg cgcacgatgc agggcaaggg ctacgggcgg gtggacctgc
     1681 gagtggacga taccgggcgc ccgtgggtgc tcgaggtgaa ccccaacccc gacctgaacg
     1741 acgatgccgg gctgtcgcgc atggcgtccg cggcgggctg ggactacccc gagctgatcc
     1801 gccgcatcgc cgagggggcg ctgcgcgagg cgcagggcgc gaaggccgcg cgtgagctgc
     1861 tcgctccgtc ccgtcgcacg cgcgcaccgc gcacgccttg agacgctggt gcgcgacacc
     1921 ggcctcttcc gcgaggccga ggtcgcgacg gcggtcgagc tgctggacga ggcgctggac
     1981 ggcgacgacg actatcgctt cctgggggcg ttcgacggct ccgagctggt cgggtatgcc
     2041 tgttacggtc cgacgccgga tacgatcggc acctgggacc tgtactggat cgccgtggac
     2101 cgcacgcgcc aggggacggg catcgggacg cagctggtta ccgcggtgga gcaccggctg
     2161 acggccgaag gcggccggct gatcgtcgtc gagacgtcct ctcgtgcgga ctacacggcg
     2221 acgcggggct tctacgaggc gcgcggctac ggcaggaccg cgacgatccc tggctactat
     2281 gcgccgggcg acgacctggt gatctacacg aaggatctga cgcatggaca tgacctggca
     2341 ggagctgctc cgtaagcgca gcatcgcctc gctcgaggcc ctggccgagc ggttcggggc
     2401 cgagcacgtg ggcgatatcg agcgcctcaa gcaggcggcg gagaacttcg agttccgcat
     2461 ctcgcccgcc atggtggacc tcatccgcga gccgggcgac cccatctggc gccagtacgt
     2521 gcccaccgtc caggagctcg agatcgagga cgggatcgtg gactcgctgg ccgaggacgc
     2581 ccactccccc gttcccaaca tcacccaccg ctatcccgac cgcgcgctgt tcctcgtctc
     2641 gccggtgtgc gccgcctact gccgcttctg cacccggcgc cgcaaggtgg gcgatcccga
     2701 gaagatcccg atggcgcacc tcgaatcggc cttccagtac ctggcccagc acaccgagat
     2761 ccgggacgtc atcatgtcgg ggggcgaccc gctgatgctg tccgaccggc gtctggacga
     2821 gatctgcggc cggctgcggg cgatcccgca cctcgagatc ctccgcatcg ggagccgggt
     2881 cccgtgccac ctgccggagc ggatcacgcc ggagctgtgc gcgaccctga agaagcacca
     2941 cccgctctac atcaacacgc acttcaacca ccccgacgag ctgacgcccg cggccgtcaa
     3001 ggggctgggc atgctggccg acgccgggat tcccctgggg tgccagacgg tgctgctcaa
     3061 gggcatcaac gacgatccgg ccgtgatgaa gcgcctgatg cagaagctgc tcgcggcccg
     3121 cgtgcggccc tactacatct atcaggccga ccaagtcgcc ggagcggagc acttccgcac
     3181 caccgtcgag aagggcctcg agatcgtgca ggcgctgcgc gggtggacca gcggactcgc
     3241 cgtgccgcac tatgtgatcg acgcccccgg cggcggcggc aagattccgc tgctgccgga
     3301 atacgtcctg gccatcgacg aggaccaggt cgtgctgcgc aactacgccg ggaagacctt
     3361 cgtgtaccgg cagccgcacg aggccggagc gacgccactg ataccgctcg agttcgagcc
     3421 ggtgctcgcc gggtgaccat gacggcgaac gcctgattcc cgcgcccgct cccgcaaccg
     3481 gagcgggcgc cgtctttgtg ccacattctc gcaggagaca acgatgcgcc tgatcacgac
     3541 ggtcgtgcgg cccgagcgcc tgccggaggt gaaagccgcg ctgttccgcg ccggcgtcac
     3601 cggcatcacg ctgagccggg tcaccggaca cggcggcgag cgcgaggtga tcgagcagta
     3661 ccgcggtagc accgtcgtcg ccgagttcca cgagaaggtg aagatcgaga tggccgtgtc
     3721 cgagccgttc gtcgagccca ccatccaggc catcctctcc tcggcccgca ccggcgaagt
     3781 gggcgacggc aagatcttcg tccagccgct ggaacgggtg atccggatcc gtaccggcga
     3841 ggccgacaac gcggcgctca cggcggtcaa cgcggacgag gtccagcgcg cggccatcga
     3901 ggcccaggcc ggcgagcgat gagccaggtc gcgcagatct cgagtggcga taccgcgtgg
     3961 gtcctcacct cgagcgcgct cgtgctcttc atgaccgtcg gcctcgcgct gttctacggc
     4021 ggcctggtcc gatccaagaa cgccctcaac acgatgatga tgagcgtcgt ggcgctcggc
     4081 gtcatcggcg tccagtgggt cttgttcggc tactcgctgg cctttgctcc gggcaacgcc
     4141 tggctcggcg ggctggcctg gctcggattc tccggcgtgg ggctcgaccc gaaccccacc
     4201 tacgccgcga cgatcccgca actggcgcat gcggtcttcc aggcgatgtt cgccgtcatc
     4261 acccccgccc tgatctcggg cgccatcgtc gagcgcatgc ggttccgcgc gtacgtcgcg
     4321 ttcctcgcgc tgtgggcgac cctggtgtac gtcccgctcg cccactgggt gtggggcgcg
     4381 ggcggctggc tccagcagct gggcgcaatc gatttcgccg gcggaaccgt ggtccacatc
     4441 agcgcgggcg tgtcggcatt ggtggcggcg cggctgctcg ggccccggca gggattccgg
     4501 cgtaccccgc tcgtccccca caacgtcccg ctcgtcctgc tcggcgcggg gatcctgtgg
     4561 ttcggctggt tcgggttcaa cgcggggtcg gcgctcgccg ccaatgggat cgccgtgctc
     4621 gccttcgtca acaccaacag cgccgccgcc gcggcgctgg tcacctgggc ggcgctggac
     4681 gggctgcgcg gcggcaaggt caccgccgtg ggtgccgcca cgggcgccgt cgtgggactg
     4741 gtgggcatca cgccggccgc cggctacgtg acgccccgtg ccgcgctggc catcggcgcg
     4801 ctgtgcgccg tcgtgagcta cttcgccatg caacggcgct cccgcagcgg cctcgacgac
     4861 tcgctcgacg tcttctcgtg tcacggcttg gccggcatcg ggggagcgct gctcaccggc
     4921 gtcttcgcca gccgggcggt caaccccggc ggggccgatg gcgtgctggc cgggcactcg
     4981 gcccagcttg gcatccaggt catcgccgtc ctggccgcgg ccggtctcgc ggcgctgggc
     5041 acgatcgtga ttttctgggt cgtgcgtgcg gtgttcggcg tgcgggctgc ggtgcgggac
     5101 gagattgccg ggctggatct cgccgagcac ggcgaggagg cctacctggg cgccgacctg
     5161 ggcggcctca cgggcccggg cgtctcgctg agtgaggggg tggtgctgca gggaccccct
     5221 ccggggagtc ctccgcagcg agccgcgtga tgtaccgccg caggaaccgg agggaggcag
     5281 aagttacggc agctgccaga tctccacccg gtttccggtg atcaccggga tcagcacccg
     5341 gttccgcttc gcgtcgtaac cgatgtcggc ggagccgtac acgccggtga tgagtttcac
     5401 ttccttgccg gtctcgtaac tggagacggt ggaatcgttc cagctggacg cgaatatccg
     5461 gttgcccgcc atagcgatgc cgtcgaagcc ccccggcccg gtcgcgacga cggtgggcgc
     5521 ccgatcctcg ggtttccagg cgagcagcgc cttcccgccg aactgcccga tgatgaaccg
     5581 cctgccgacc gggtccaggg cgatcccgtt gggccacccc agcgagtcgc cacgcagggc
     5641 gacggtcgcc gtccgatcgg gcccgatccg gaacacccgg tcggggccgc gatgcagcac
     5701 gttcccgacg tcgtcgaacc ggatgccggt gtcggtgatg taaatggcgc ccgacggcgt
     5761 cgcggtgatg tcgttcagaa agacggcggt cagcgtgctc aggctcacgc tgtcgagcgg
     5821 agcgccggtc cgagcatcga acgcgcgcac cgcgtcgatg tcggtcaccc acagggtgtc
     5881 ccccgcgagg gtcatcccct tgggggcgtt caggacgacg ccgttcttcc cgccctcgat
     5941 gaaccggagg ctcacgaccg cgccctccgg cgtcactcgg ctgatgaacc cgttgccgtc
     6001 cttcgcggtc ggactgccgt tgatgttcga gacgaaatac acgtcctgag cggagtcgtg
     6061 cagcaccgac tcggggttcg agaagcccgg caccgcgctc acccgcgtcg ccgtcggcgc
     6121 aggagggggc gcgggggtcg cggaatcctg tgacgccgtg gaacccttgt cgatcggctt
     6181 gcacgccgcc aacacgagac cgcagcagag aaaccgggcg agccttggca tcatcgcacc
     6241 tccggccgtc gtcctcatgg gagctgccag atctccacgc gattcagcgt gaacagcggg
     6301 atcatgaccc ggttgcgcct ggcgtcatac ccgaagtcgg ccgggcccgg cacacccgag
     6361 atcagcttga ccgcgttgcc gctctgatat gccgagatgg tcgaatcggc ccagctcgag
     6421 acgagcaatt ggccacgtgc gacctcgacg ccgtcgaagg agcccggacc ctgcgcgatg
     6481 acggtcggca tcttgtcacc gagcttccac gcgaacacgg tgggcccacc gaacgccacc
     6541 acgatgaagc ggttcccggt cgcgtcccag gtgatgccgt tggggcgacg caggctgtcg
     6601 ccctccgccg ccacgctcac ccggcggttg gacgtgaccc ggtagatccg gtccgtcccc
     6661 gggtgggaca tggtgcccgc ggagtcgaac tggatgcccg tatcggtgat gtagagggcc
     6721 ccatcgggac cgaacacgac atcgttcagg aagacggcgc cctgcgcgcg cagatccacc
     6781 atccccaggg atctgccggt cttcgcgttg aaggcgcgca ccgcgtcgat gtcggtcacc
     6841 cacagcgtgt cgccgtgcag ggcgagcccc ttcggcgcgt tcagggtgac gccgctctgc
     6901 ccgccggcga tgaagcgcag gttctcgacc ccgctgtccg gcttcacgcg gctgatgaag
     6961 ccgttgttgt ccttcacgtt ggggttgccg ttgatgctcg agacgaagta cacgtcctgc
     7021 gccgagtccc acagcaccga ttccggcgtc tgcaggctgt cgatctgcgc caccttgctc
     7081 gccgtgggag gcggaggggg ctgcggcggt ttcggttgtt ccttcgtgca agcggccacg
     7141 gccgccacgc agagcgcggc gatcccgagc gttcgcatgt cgattctcct gatcggcggt
     7201 ggttttcgcc ttctcgttac ccggccgcgc cggggtgttc ccgtcagaga cgctgcagca
     7261 tggcctggaa ccgcggctcg gcccggagcg gatccagatc cgaatcgttc tcgagccatt
     7321 ccttgtgccc gaagccattc tggatcgcct tctcgatgca cgcgagggcc tcttcaatct
     7381 cccctgcgtt ggcgaaccca cacgccacgt tgtagagcac cgccgggtct tccgagtcca
     7441 tggcctgcgc ccgtcggttc cattcgagcc cccgctctcg ttcgtccaac tgcatcagcg
     7501 cgccggctcc gagatacaac gcccgaacgt catcggggtt gagctccaga tgccgctgcg
     7561 caacctccag cccacgccgg agggccgcca ccatatcctg ctggcgaccc aatcccttca
     7621 acggcgccgc catcaggatg gggacctgat agtcctcggg ccgcacgcgc gccgcatctt
     7681 cgtaatgccg gactgcctcc tccaacttcc cttcctggaa gcacgcccgc gcgtacagat
     7741 agtgcgcctc gaactgcttc gggttgaggc ggatcgcggt ctcgaactcc ttccgtgcct
     7801 cgtcgtaccg cctgctcagc gagacggcga acccgcgcga gccgtgcgcc tcggccagct
     7861 ccggatcgag ctccaacgcc ttccggctcg ccgcctccgc cccttccagg ttggccttgc
     7921 tcccgtccca gtacatgtac agaaacgagc agcaatcggc gacgccggcg taggcgaggg
     7981 cgtagcccgg atcgatctcg atcgcccgct cgaacatgcg acgggcgaac gcgatgctgg
     8041 tccggcggaa ctgatggaag aactgtcggc cgcgcaggta gcagtcgtag gcctgcacgt
     8101 tgtcggtcgg cgccttctcg atggcccgct tctcttggtc gctgagcacg actctgagcg
     8161 cggtcacgat gttgcccgcg atctcgtcct gaatcgcgaa cacgtcctgc agctcgcgat
     8221 cataccgctc ggaccacagg tggtacccgt cggccacgtt gacgagctgg gcggtgaccc
     8281 gcagcctcgt gcccgccttc cgcacgctcc cctcgagcac ggtggcgaca ctcagctgct
     8341 cgcccacctt gcggatgtcc aggttcttcc ccttgaaggc gaacgccgag gtgcgcgccg
     8401 ccacgcggag cgcctgcacc ttcgtgagcg cgttgatgat ctcttcggcg atcccgtcgg
     8461 tgaagtactc gttctccggg tcggcgctca tgttgacgaa cggcagcacg gcgatcgact
     8521 tgacggccga ggcggcgggc gccgtggggg tggcaccgga gggagtggtg acacccgacg
     8581 ccgtgagcgc ctgggagaac tgcgccgcgg tggtgaaccg cgcagccggg tccttcgcga
     8641 gcgccttcat gaccgcctgc tccgcccact cgggcgtgcc ctccgccggc cggaccgagc
     8701 gcagcggtgg cggcggctcg gtgaacagct gggcgatgat cgcctgcgcc gtcgggcccg
     8761 taaacggcgt gcggcccgtc agcatctcg
//
LOCUS       DBNGZW010000779         5371 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate MAG05514
            SRR34280900_k127_1772569, whole genome shotgun sequence.
ACCESSION   DBNGZW010000779 DBNGZW010000000
VERSION     DBNGZW010000779.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414922
            Sequence Read Archive: SRR34280900
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Pseudomonadati; Gemmatimonadota; Gemmatimonadia;
            Gemmatimonadales.
REFERENCE   1  (bases 1 to 5371)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5371)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 01:43:57
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,645
            CDSs (total)                      :: 3,615
            Genes (coding)                    :: 3,592
            CDSs (with protein)               :: 3,592
            Genes (RNA)                       :: 30
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 29
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 5 of 23
            Pseudo Genes (incomplete)         :: 14 of 23
            Pseudo Genes (internal stop)      :: 6 of 23
            Pseudo Genes (multiple problems)  :: 2 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5371
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR34280900_k127_1772569"
                     /isolate="MAG05514"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /geo_loc_name="China"
                     /lat_lon="32.253958 N 114.041348 E"
                     /collection_date="2022-06-03"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            598..1017
                     /locus_tag="AC16JC_18210"
     CDS             598..1017
                     /locus_tag="AC16JC_18210"
                     /inference="COORDINATES: protein motif:HMM:NF014176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HIR5166694.1"
                     /translation="MTQPVAFNHRKHTEQLKIACQLCHQYETTGAHAGLPSAQTCVMC
                     HQVRQGTSPEAARLTVLLARGDSLRFNKLFHLPPYVYFTHRRHVGIAKLPCEKCHGAI
                     AHTERPPERPLLGIRMRVCLRCHQLAGQSVDCVACHR"
     gene            1030..3402
                     /locus_tag="AC16JC_18215"
     CDS             1030..3402
                     /locus_tag="AC16JC_18215"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-containing oxidoreductase family
                     protein"
                     /protein_id="HIR5166695.1"
                     /translation="MTPNRREFLWTAGAAAAAAACQLKDYQWSSPQEQEAGWAPGIEE
                     RRSSVCLVCPARCGIRGRTVDGRLVRVTGNALHPMSQGGLCPRGVGGVQMLYHPQRIA
                     APVVRAGARGAGQWRGISSQEAVAQLAQHLGALRTAGRPERLAVVAGYCAGTMDELWR
                     QFLRAFGSPNYVTDGYPDGAEAVMGLMHGIARRPAYDLEAARLVLSFGAPLFESWWSP
                     VQAFVAFGGSAARPSRHGHFVQVDTRFSRTAARAHEWVGVPPGTHGVLALGLAYVLIK
                     EQTFNADFVARHVTGFEDGVDAAGRPRAGYRSLVLRNYRTEEVSAITGVPVERIVALA
                     RAFAAGPGAVAVCGSDVLYAPDGLLSGLAVHSLNVLMGNISRPGGVMFGDDPPLAPLA
                     AARLDAVARAGLARAPVAHAAVPFGMGDPAPQFAAAAARGVGPIEALLLYYSNPLASA
                     ARTDDWRAALERIPFVVSFSPFLDETARFADLVLPDLLPYERWQDAPTPASYPYQVWG
                     LAQPLVAPPDKTLTTADALLALARALKGSVASSLPYPDMPTLLQARARGLFAAGRGMT
                     LGDMFERTHYRQMEERGWWLAEQPDFDAFWKAIVEQGGWTDLLYDGTEPARLARTASG
                     RIELMPETLLRALRDEGKDRLPYAGLRASTTPAPAGTAPSTYPLRLVPYRMSTLSSGT
                     IHLEPWLAEQPSLTLDVHWLPWIEVHPTTAAALGLGDGTMVWVVSARGRYRARLRLFL
                     GTAPGQVNAPYGLKHPGGELANPLQLLDGATDPLTGLAAWYSTFVRLERA"
     gene            3416..4099
                     /locus_tag="AC16JC_18220"
     CDS             3416..4099
                     /locus_tag="AC16JC_18220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010937996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HIR5166696.1"
                     /translation="MTRWGMVIDLDRCTGCGACEVACKSENNVATVTPDQADMGRVFA
                     WMRVMTEVTGDFPNTSMRFMPRPCMHCDNPPCVQVCPVGATYINPEGIVGQIYPRCIG
                     CRYCANACPYTVKYFNWFVPNWPVSERAHLNPDVSVRPKGVIEKCTFCDHRLQRARED
                     ARAEGRELREEDYQPACAEVCPTGAIVFGDLENPRHRVQALKGDPRAMRLMESLGTEP
                     KVYYLVPRA"
     gene            4101..>5371
                     /gene="nrfD"
                     /locus_tag="AC16JC_18225"
     CDS             4101..>5371
                     /gene="nrfD"
                     /locus_tag="AC16JC_18225"
                     /inference="COORDINATES: protein motif:HMM:NF015851.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HIR5166697.1"
                     /translation="MRPEIAAKLEQLPPDERTLLRPLVHTTSRFYVAAGILGVVVLWG
                     ITAYVLQWRYGLGRTGLNRPEYWGIYIIDFVFFIGISHAGTLISAILRVSKAEWRRSI
                     TRSAEFITVLVLGFGAINPIIDLGRPDRLTNVFFHPHLRSPLLWDIMSICLYFTASTI
                     YLYIPMIPDLAIVRDTGIKAPWFYRFLAAGYQDTPAQRARLERVIGVLAIAVIPIAVS
                     VHTIIGWIFAMTLRPMWHSTIFGPYFVMGAIYSGIAAILIAMVILRRAYGLGQYFKPV
                     HFDYMGRLLLIFSLLWFYFTFAEYLTAFYGKEPAEMRTFWAKVAGPFAVPFWLMVICC
                     FVIPLALMARRAMRTPRGTLIAGIAVVIGMWLERFNIVVPTSVNPRWEMESLGHYLPS
                     WVELSILAGTFAGFVLLYMVATKFFPIVSIW"
BASE COUNT          798 a         1844 c         1814 g          915 t
ORIGIN      
        1 tcggaactgc accgccaggt acagcgcgca cggatgcccg agcagtcgga acagcaatgg
       61 tggcacagac gatgaccaaa gacgacaacc accccgagga ggcccgacaa cggacgacac
      121 gaacacgcgt gaatgccgac attttgtcgg cgttggatgt cggggactca aacctctgga
      181 tcaggagagt cctggttcga gcccaggagg ggcaactgag atcgcgtggt ggttgctggg
      241 gttagcgccc tggcgatcgc cgcgcggtct ctttttggcg gtgcgtcagt gactgtgtca
      301 gtggctgacg cggcttcggc tgcgaatgac ggttcatcaa gccttcatgg tttcttccgc
      361 aaacttgtcg tagcaatacc tgaagtctcc ctcacacgct atggctcacc gggcacggtg
      421 agttgaaaca gcccggagcg ccggatcgca cctggagggc aagtctccac tctgaggaag
      481 gtcgcggcgg gcatctctgc acggctcggg tcggcgcccc ggctcaggac cggtgtggcc
      541 atcgccctgc ccatcctcct ggtcgccact gcgctcgtct ctttccgaac gccccgcgtg
      601 acgcagccgg tggccttcaa tcaccgcaaa cacaccgagc aactgaagat cgcctgccag
      661 ctctgccatc agtacgagac cacgggcgcc catgcagggc tgccgagcgc ccagacgtgc
      721 gtgatgtgcc accaggttcg ccagggtacg tcgcccgaag ctgcgcgcct gacggtcctg
      781 ctcgcccgcg gcgactcact gcgattcaat aagctctttc atcttccacc atacgtgtac
      841 ttcacgcacc gccggcacgt aggaatcgcg aagctcccct gtgagaagtg ccacggcgcg
      901 atcgcccaca ctgagcgccc tcccgagcgg ccactcctcg ggattcggat gcgggtgtgc
      961 ctccgctgcc accagctcgc cgggcaatcg gtcgactgtg tcgcctgcca tcgataggag
     1021 cgagcgcccg tgaccccgaa tcgtcgcgag ttcctgtgga cggccggagc ggccgcggca
     1081 gccgcggcct gccagctgaa ggactaccag tggagctctc cgcaggagca ggaagccggc
     1141 tgggcgcccg ggatcgagga gcgaagaagc tccgtctgtc tcgtatgccc ggcgcgttgc
     1201 gggattcgtg gccgcaccgt ggacggccgg ctggtccgcg tcaccggtaa cgcgctgcac
     1261 ccgatgagcc aggggggact ctgcccgcgc ggcgtcggtg gcgtccagat gctctaccat
     1321 ccgcaacgca tcgcggcccc ggtggtgcgg gcgggcgcgc gcggcgccgg gcagtggcgc
     1381 gggatctcgt cgcaggaagc ggtggcgcag ctcgcccagc acctcggggc gttgcgcacc
     1441 gcgggccggc cggagcggct ggcggtcgtg gcaggctact gcgccggaac catggacgag
     1501 ctgtggcgcc agttcctgcg ggcgttcggt tcgcccaact acgtgaccga tggatacccg
     1561 gatggggccg aggcggtgat ggggctcatg cacggcatcg cgcgccggcc ggcctacgat
     1621 ctcgaagccg cccggctcgt gctctcgttc ggcgccccgc tgttcgagtc ctggtggtcg
     1681 ccggtgcagg cgtttgtcgc cttcggcggc tcggcggctc ggccgtcccg gcatggccat
     1741 ttcgtgcagg tggatacgcg cttctcgcgc actgccgctc gggcccacga gtgggtcggc
     1801 gtgccgcccg gaacgcacgg ggtgctggcg ctgggcctcg cctacgtgct catcaaggag
     1861 cagacgttca acgccgactt cgtggcccgg cacgtaacgg ggttcgagga tggcgtggat
     1921 gccgctgggc gcccgcgcgc gggctaccgc tcgctcgtgc tgcgcaacta ccggaccgaa
     1981 gaggtgtcgg cgatcaccgg ggtcccggtg gagcggatcg tggcgctcgc tcgggccttc
     2041 gcggctggtc ccggcgcggt ggccgtatgc ggcagcgacg tgctgtacgc gcccgacggc
     2101 ctgttgagcg ggttggcggt acacagcttg aacgtgttga tggggaacat cagccggccg
     2161 ggcggcgtga tgttcggcga cgatccgccg ctcgcaccac tcgccgcggc gcgcctcgac
     2221 gccgtcgccc gggccggtct cgcgcgagcg ccggtggcgc acgccgcggt cccattcggg
     2281 atgggcgatc cggcgccgca gttcgccgcc gccgcggccc ggggtgtcgg gccgatcgag
     2341 gccctgctgc tctactacag caatccgctg gcgtccgccg cccgcaccga cgactggcgc
     2401 gccgcgctcg aacgaattcc gttcgtcgtg agcttctctc cattcctcga cgagaccgcg
     2461 cgcttcgcgg atctggtgct gcccgatctg ctgccctacg agcgctggca ggacgcgccc
     2521 actccggcct cctacccata ccaggtatgg gggctggcgc agccgctcgt ggcaccgccc
     2581 gacaagacgc tgaccacggc cgacgcgctg ctggccctcg cccgggcgct gaaaggcagc
     2641 gtcgcctcga gcctccccta cccggacatg ccgacgctgc tccaggcacg cgcccggggg
     2701 ttgttcgcgg ccgggcgcgg gatgacgctg ggagacatgt tcgagcgcac ccactaccgc
     2761 cagatggagg agcgggggtg gtggctcgcc gagcaaccgg acttcgacgc cttctggaag
     2821 gcgatcgtcg agcagggcgg gtggaccgat ctgctgtacg atgggaccga acccgcccgg
     2881 ctggctcgca ccgcgagcgg acgcatcgag ctcatgccgg agacgctgct gcgcgcgttg
     2941 cgggacgagg ggaaggaccg gctgccatat gccgggctca gggcatccac cacgcccgcc
     3001 cccgccggga ccgcccccag cacgtacccg ctgcgcctcg tgccctaccg catgtccacc
     3061 ctcagctcgg gcacgatcca cctcgagccg tggctggcgg agcagcccag cctcacgctg
     3121 gacgtgcact ggctgccgtg gatcgaagta catcccacca cggcggcggc actgggactg
     3181 ggcgacggga cgatggtgtg ggtagtctcg gcgcgcggcc gctaccgggc gcggctgcgg
     3241 ctgttcctcg gcacggcccc cgggcaggtg aacgcgccgt acggactcaa gcaccccggc
     3301 ggagagctcg ccaatccgtt gcagctgctg gatggcgcga ccgacccgct gacagggctc
     3361 gccgcatggt actccacgtt cgtgcgcctc gagcgcgcgt gaggagggcg actccgtgac
     3421 gcgctggggc atggtgatcg acctggatcg ctgcaccgga tgcggcgcgt gcgaggtcgc
     3481 gtgcaagagt gaaaacaacg tcgccaccgt gacaccggac caggcggaca tgggccgggt
     3541 gttcgcctgg atgcgcgtca tgacggaggt gacgggcgac ttccccaaca ccagcatgcg
     3601 gttcatgccc cgaccctgca tgcactgcga caatccgccc tgcgtccagg tgtgtcccgt
     3661 gggggccacc tatatcaacc ccgagggcat cgtggggcag atctacccgc gctgcatcgg
     3721 ctgccgttac tgcgccaacg cgtgtcccta caccgtgaag tacttcaact ggttcgtccc
     3781 gaattggccg gtgtcggagc gcgcccatct caatcccgac gtttcggtgc gccccaaggg
     3841 cgtgatcgag aagtgcacgt tctgcgacca ccggctgcag cgggcccgcg aggacgcccg
     3901 cgccgaggga cgggaactgc gtgaggaaga ctaccagccg gcctgtgcgg aggtgtgccc
     3961 caccggcgcc atcgtgttcg gcgatctcga gaacccgcgc catcgcgtgc aggccctcaa
     4021 gggcgatccc cgcgcgatgc gcctgatgga gagcctgggg accgagccca aagtctacta
     4081 cctggtgccg agagcctgat atgcgccccg agatcgccgc caagctcgag cagctgccgc
     4141 ccgacgaacg gacgctgctc cgtccgctgg tgcacacgac ttcacgcttc tatgtcgccg
     4201 ccgggatcct cggggtcgtc gtcctgtggg ggatcaccgc ctatgtcctc cagtggcgct
     4261 acggcctggg ccgcaccggg ttgaaccggc ccgaatactg gggcatctac atcatcgact
     4321 tcgtgttctt catcggcatc agccacgcgg gcacgctgat ctccgccatc ctccgggtga
     4381 gcaaggccga atggcgccgc tccatcaccc gctcggccga gttcatcacg gtcctggtgc
     4441 tcggtttcgg cgcaatcaat ccgatcatcg acctggggcg tcctgatcgc ctgacgaacg
     4501 tgttcttcca tcctcacctg cgctcgccgc tgctgtggga catcatgagc atctgcctct
     4561 acttcacggc gagcacgatc tacctctaca tcccgatgat cccggacctc gcgatcgtgc
     4621 gcgataccgg catcaaggcg ccgtggttct accgcttcct ggcggcgggc tatcaggata
     4681 cgccggccca gcgggcgcgg ctggagcggg tcatcggcgt gctcgccatc gccgtcatcc
     4741 cgatcgccgt gtcggtgcac accatcatcg gctggatctt cgccatgacg ctgcggccga
     4801 tgtggcacag caccatcttc gggccgtact tcgtcatggg cgcgatctac tccggcatcg
     4861 ccgcgatcct gatcgcgatg gtgatactgc gacgcgccta cgggctggga caatacttca
     4921 agccggtgca cttcgactac atggggcggc tgctgttgat cttcagcctg ctgtggttct
     4981 acttcacgtt cgccgagtac ctgaccgcgt tctacggcaa ggagcccgcg gagatgcgga
     5041 ccttctgggc caaggtggcc ggcccgttcg ctgtgccgtt ctggctcatg gtgatctgct
     5101 gcttcgtgat cccgctggcg ctgatggcgc gccgcgcgat gcgcacgccg cgcggaacgc
     5161 tgattgccgg catcgctgtg gtgatcggga tgtggctcga gcgcttcaac atcgtggtcc
     5221 cgacatcggt caacccgcgg tgggaaatgg aatcgctggg ccactacctg ccgtcctggg
     5281 tcgagctctc gattctggcc ggcaccttcg ccgggttcgt gctgctctac atggtggcca
     5341 cgaagttctt cccgatcgtg tcgatctggg a
//
